LOCUS       DAORRQ010000001       174241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1_length_174241_cov_173.239629, whole genome shotgun sequence.
ACCESSION   DAORRQ010000001 DAORRQ010000000
VERSION     DAORRQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 174241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 174241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..174241
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1_length_174241_cov_173.239629"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(616..691)
                     /locus_tag="PK224_00005"
     tRNA            complement(616..691)
                     /locus_tag="PK224_00005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Thr,seq:cgt)
     gene            complement(782..1933)
                     /locus_tag="PK224_00010"
     CDS             complement(782..1933)
                     /locus_tag="PK224_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB14152.1"
                     /translation="MLIGRTRWPRARRILFLACLWLASVSCSIIVLEWVTGYLYERTP
                     FSNGKRIIDLSLGQQPTVRSDVNGESASLASHPYLLYTHLPNLTTDGYQQTNSLGYRN
                     REFTVAKPPDTIRILVLGGSTTFMWPFVKNPADTWVARLEAKLKTISKKTVQVVNAGL
                     NYGTSAEALAGYVFRHRFLQPDIVIYHGGGNDVLPLFFGNYTPEYTHFRRYGAGEIPR
                     PGEKFLLQHSSTAKYVYSRWLEPIGAVVVTEPFWEVDPKTALERVRMQAPEGFRRNLD
                     MLVSSSQQAGSAVVLFGFLQARRQNLSKNAGAFKGFEEALILGLEKNYDVMREIATRY
                     RVPFVIPPQDRFEDEWFQDNCHLSPAGEEVKAQIMFEQLRDHPIFLSAL"
     gene            complement(1967..3910)
                     /locus_tag="PK224_00015"
     CDS             complement(1967..3910)
                     /locus_tag="PK224_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14153.1"
                     /translation="MRRLSRCPLAFLLFVVWVSSGCQFFGQRSSLATHYNLPLTVQLR
                     MDHSIAAASLDYRDACGQALTLPIRDGLETQLKKRMGQVFERVHLEPGPSTGAVDGVV
                     DTALGVRQVDLFIPRKANKSYPATVALGMEFSYTDHHGTVLVSKKLQSAATGEVETRA
                     DTCNISGLDKVAEEAAAKLVEGMAQQLGTATKIREQAQFRKSGDKGTMQSAPVPPATP
                     AVAPLAATPVVPVAPLQSAAGSTPQAAAPDSAIRSGMTQLSFRTIIRDDNQNHVLEQR
                     ESFSVEFEVKNEGAVVAEGVEVELSGHAAIVGGLKSPVSLGTLQPGEVRRVAVDGKVG
                     AVADAEQAELMCALRASSNVLLPSAKKFFVAIHPDAGAAVEVLSVDVDQLPSRNGKSA
                     QPNSVGIAIGIGAFRDPAVPTVKFAAHDADVMGGYFKTVLGVPPHKVKVLSDANALRD
                     DLIEVFEQWLPKQGSPQATVYIYLSGRAVVDQETGIVSLLPYDGTRAGASRMFSLARL
                     QRALTKASVKQAVLMLDLSLEPSSGFDPGRVVPPRWAQQDSSGEPDRVMLMIGNSGLQ
                     EAQAYQPGQHGLFTYFLLKGLRGAADLDKSGTVLSGELCEYVHDQVDAVTRGATGGGQ
                     PTLCLPMGGRQPVLTGIPLSKIR"
     gene            4095..4940
                     /gene="ubiA"
                     /locus_tag="PK224_00020"
     CDS             4095..4940
                     /gene="ubiA"
                     /locus_tag="PK224_00020"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRB14154.1"
                     /translation="MADLIRLRNQSGTWLLLLPSLWSLVLANQGRPPMWLLGVFTLGA
                     FVMRSLGVVFNDLADRNFDKHVARTRTRPLAAGRLAPRQALLVALGLAVIAAALVITL
                     NPFTMLLSPIALFLAAIYPFSKRWIQIPQGVLGIAFGWGAVMAWAASRGSIEAPAWWL
                     FAATTCWAIAYDTIYALQDCEDDRRIGVKSSALLFGEAVPVAVGVFLVGMVLCLVVAG
                     QLSGLGMGYYLILALPTGVFLWQVRRLQAPVTPHIAFAMFKQHVYSGVAILVGIWIGT
                     FNQTP"
     gene            complement(4945..5916)
                     /locus_tag="PK224_00025"
     CDS             complement(4945..5916)
                     /locus_tag="PK224_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB14155.1"
                     /translation="MVVGLSGLIGAGGCSMFQNEQSAKGKKLYAHYCMHCHGENGRQN
                     EGFNWSSMTDPKPKDLSNKDEMGTFKDEEIFNTISRDMKDTGPGGEKIGDDEFAVPTM
                     PTFKYTLSEEEIWGIVGYVRSLHGKKLEFNVEGRKKDLQAAQQAADQKFKEAERVAQE
                     AEKKASDEADKKGVEVDDNAYAKEMQAMGQAKKELDQATAALANFTTRPGKGVNIARP
                     DLNMKPDAAAKLVEVGKQIYVTKYGCNGCHKIGDEGGKVGPALDRAGYRLNPTWVYRW
                     LRNPQAMKPDTRMPSLGLNDADAKAVALYLKTLRAPKPDQPLGKVGE"
     gene            complement(5961..6788)
                     /locus_tag="PK224_00030"
     CDS             complement(5961..6788)
                     /locus_tag="PK224_00030"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRB14156.1"
                     /translation="MARVRSVGKRWWVGAVGLLGLCVAAEVQPAYSQQSVSVRAALVK
                     GSLPADDPTAAAWATAAVSEFPMSPQVHWPVRITDVTAKSVKVRGLHDGTTVAILLEY
                     SDPSEDPEDAAALEFMVGDTKAHFAHGQPMAQVEGGPVNIWYWKNKDGKGADLGAKGF
                     GTLKPHAHQDVKAKGVYQGGVWKVVFSRPLSTEHVAEDTQFKPGTFASIAFAVWDGKK
                     METGQPKEKGSEKAISSWWYFRADAPPDYSPYMYALLAVALALGFELVLVRRLKKGS"
     gene            complement(6829..7554)
                     /locus_tag="PK224_00035"
     CDS             complement(6829..7554)
                     /locus_tag="PK224_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB14157.1"
                     /translation="MGGVLVRPIILTVCIYLFLKFIVPNLPHSAPLPSSLIFLYLMLT
                     TAGIVIFATLSGASKDAFFGPILRFLTGEGGGALSGARYLVLALFPLLVGWQAYGSTA
                     SSDAPPGENRTIHPAPPGEYTGLSNPVPKTLDNINYGKGLYASRCSPCHGNFDGKGFA
                     AAGFTPPPANFKDPTTIAMLQESYLFWRIKKGGVGLPVEGMPWKTAMPRWELEMSDED
                     IWKVIMGEYDGAGQKPRTWDESE"
     gene            complement(7582..8202)
                     /locus_tag="PK224_00040"
     CDS             complement(7582..8202)
                     /locus_tag="PK224_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB14158.1"
                     /translation="MSEVVKLQLIGLCVVGLGTVILLFIKGQFIRVIGFVAIVLGLFS
                     LVALAVPQMASLPPAEESINIADIKTPTDMATIGQKIFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSREFIFESLTEPQAYLYLDYRHEGVPKEYPARMPYINKNPIGLSKNEI
                     LSVIAFLQQMSGEPISVSPTELDLPRATPAPVKAADAGTLAVAQAR"
     gene            complement(8269..10149)
                     /locus_tag="PK224_00045"
     CDS             complement(8269..10149)
                     /locus_tag="PK224_00045"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB14159.1"
                     /translation="MMNQGQIVTNHGSQIAGRVALLAGCAALLAWVGMPDAALAQEAA
                     PQAPTAYRDIPYIGSRNLVWIIAQLHLLLAGFVLGVPIFAWLCEIVGWKSGDKRYDKL
                     AKEFTKLLTSSYATTALFGGILLFLLIGFYPKLMAYLTDIFFPSFLVYCGLFLVETAT
                     LYLYWYGWDAMAEGSGKKFHIFLGFLLNVFAFFIMIVPNSWATFQSSPVVISEGSDFA
                     RAWAATWNPTWWPVNIHRLIANVVLGGYICGAYAGIRYLSAKSQEERYHYDWMGYVGN
                     FIGVFGLLPLPFAGYWLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYY
                     FWLGITHRIPGSETQYRKPIMAMLIILLLCLGVWMTPHSLVASLEEAQKMGGTHHPLL
                     GVFGVMSAKMTVSNLMVLVTFMSFIMYWRAGKQDTAGWAKAAKALMGGVLGLAAIAVI
                     VLGVWGYFVPAIIRINYFSTSQVGIVIFVMLTITPLTALLLKSAKTTTEMVWGRMPPR
                     AGYALVLNAVMVILLMTLMGYARSSSRVHWHIYGVMRDSSQYAFSPALGYAAALMALN
                     TFLFCMLVAFIFWVATMGDKAKAAASTKGSVEPGAIPAMAGGAPALDGPEVDASEEKR
                     PV"
     gene            complement(10178..11182)
                     /locus_tag="PK224_00050"
     CDS             complement(10178..11182)
                     /locus_tag="PK224_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14160.1"
                     /translation="MSSFKLGLSILWPACWTALPIKMAFAMLFMAMGTIHLETKLGIT
                     FLMLLMTPVSVFAFFVISLGVGFHFGEGIGLPLLFLLSIPIDIWSLGLVARTVFLERL
                     RLEPPDSLGIGLWVRFAVAGAIYLPFLWVVQGGATDLARSITKSILDMDMLKAMPVAE
                     RIGLEFTAWGSAAFVVLLALTFVGLSILGKLVEGRAAGAQPAGDTYQDLISRWDMIRV
                     PADQSLMLTAFTAAGAVLSLLFWAVLPVSTPHPHECCKPATVEAAPPFDAQKSLSKGE
                     KAFKDAELKIAAIEQRTAEDEKDKQKGGKDKAGVKDGSPKSDGKPSQSAAGKPAGSGK
                     "
     gene            complement(11219..12334)
                     /locus_tag="PK224_00055"
     CDS             complement(11219..12334)
                     /locus_tag="PK224_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HRB14161.1"
                     /translation="MDTTTQPTTTQPSAIEKVFAFVDERVGLKTLQAKMLNEPVPGGS
                     RWAYVFGSVLLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHSVDYGWFLLSYHFW
                     GSTAMVVCVFAHMSQVFLWGAYKKPRELIWLVGLALFGIVMGFGFTGYLLPWDQRAYW
                     ATTVGVEIMDKTPLLGDFMARFLKGGATPGQMTLSRFFVIHVMVLPAALMGLAGLHLF
                     LFRKAGPAGPFRGSVEEIKAKTDYFFPRQIWKDIVGMVAVFLCICSLAFWEPIVLLEE
                     ATPDPGDYHPEPEWYFLFLFQLLRLKVFAGEFGQFLGAIALPGAFMALLAALPFIDRS
                     PERNIFKRPIALIGWIVVMASILIFTVAAIINREFLE"
     gene            complement(12335..13273)
                     /locus_tag="PK224_00060"
     CDS             complement(12335..13273)
                     /locus_tag="PK224_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRB14162.1"
                     /translation="MQPKLKSKVRCTDGEVGEVRRVIMDPLSHEISHIVVGSGAVDSV
                     ERQVPMGQVQGVAEEVVTLRGTSAEYATLPVFKRDDYVTTHEVEIAHLEERIHVTPGE
                     VLVPFPELERSVKRRTFFANFTHAIGFLIGLPIAFPVLRYLMKPMYSPFDNQWLKIGN
                     SGKIKQDDVGVQFKFKRKIKEAYMPEQEIDKNVWVLKATPKVLETIYQGKDMEFHDAS
                     GKVIWTNKKDVPYIAYSGKCPHLGCGFKWRTHKTLGQVFLCPCHLSIYDAAGKVLDGP
                     APRALDPLPIKVTAAGDITIIDMEFKAGTKAQVRIV"
     gene            complement(13279..14085)
                     /locus_tag="PK224_00065"
     CDS             complement(13279..14085)
                     /locus_tag="PK224_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB14163.1"
                     /translation="MGNVIKKSLVGIVIGGILFGATNALGFPFVFQVLFFGYAMLGAV
                     VFIILDLPPLKPISGGKAAGALVVFYAILSVAYIAGGAVWPQFDPEDEKGKIDKILKP
                     KREHYEAGKVEVLLQRAKALDEKAKELTARLQALGAAQAPADQKAGGEAAPTATTAAA
                     GGDIAKLGEEQWQLQECYNCHKLNGEGGKKRGPELDNIGNLLSAEEIATKILDPKSYK
                     AEGFEKEYEKGKMPDKYKDLMEPKDVQALAGWLASYKNASVNTPKPIKMK"
     gene            complement(14125..15828)
                     /locus_tag="PK224_00070"
     CDS             complement(14125..15828)
                     /locus_tag="PK224_00070"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB14164.1"
                     /translation="MKIWQRCLLVMFALVAILGGVAYAQAPSAPPVEFPYTGNRTAVW
                     IVAQLHILFAGFILGAPIFVVISEWLGYRKQDPRYDRLAKEVTKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLIFAVVYPLLFIGETIILYMYFYTWDAWKGEKKARH
                     IALGVLLNLIGSITLFVIDGPTSFMNTPVRAEGVSPAEFLATASLWDKVFNYSWMPLN
                     LHRLVGNVTFGGFIAGLIAAYMFMGSKKEEERAYYDWMGFVGNMIGVGALLFLPFMGY
                     LLSYELCDYDASICPYMMADQLSMFFEMQGAMVGLIFLASNYYIWLSMKRIEGVERVR
                     MTVIAPIVMVLLPIVMTKVLTDYPVPDATSLAFLVPLLLAPVILGRFIPLTVSSSTVI
                     KVGFLMVVVGNAIWMTPHGFVPTGAKLVAELELPSDWNFLALMPAKNSAAFTLIFVTV
                     VNYVIYNRAVSQGTIVWGKIDFASQFVLVFLAFSAIWTMGLMGAVRSLLRKYFHAYNL
                     LPDFTAESFTPTLSYSAWWITGITVTFFAVVSFAIIVTLRPSDSKGHAHEGSPVPAGA
                     K"
     gene            complement(15894..16397)
                     /locus_tag="PK224_00075"
     CDS             complement(15894..16397)
                     /locus_tag="PK224_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB14165.1"
                     /translation="MKQAGMKRLALMVGILGFGFGVAACGGGEGGGEGPVVPPPPAPA
                     EYADKHMPAGWWTDPKIIEEGKELYIGGKNPDVNCASCHGKDGKPVKAGARDFRNGDR
                     MKLYSDSVWFWRISEGVPNTKMKPWKSKLSEEDRWKILAFERSFGLAGKGWDANKKDW
                     VPADQVK"
     gene            complement(16609..17148)
                     /locus_tag="PK224_00080"
     CDS             complement(16609..17148)
                     /locus_tag="PK224_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB14166.1"
                     /translation="MRWRRAISRKTAWRVVVVWGLISTLFVPSVLFASHAADHRFTVE
                     GFVCGKDGRPGANVEVLVKDTRITVGQTVTTDGDGYYKVTLHLHNDNVGDPLLVEVGG
                     EQKNYKIQFDPNDLETERKLRVDFGSGCERDLGPPQWLLYGLGIAAVAIVGWIGLKIS
                     RKRMREEQRRKKGQGKRQK"
     gene            complement(17432..17821)
                     /locus_tag="PK224_00085"
     CDS             complement(17432..17821)
                     /locus_tag="PK224_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14167.1"
                     /translation="MTDAKYHAPETGSLEGRVFRPASLTELAEAIELAFDYRGDITVE
                     LKSGGHVTGYLFNRTATGEQPTIEIFPAASSGTLTIPFSEMVAIAFTGEDTATGKSWE
                     AWSAKKESERRKDIDRAAAEAKSRGHL"
     gene            complement(17923..18939)
                     /gene="hpnH"
                     /locus_tag="PK224_00090"
     CDS             complement(17923..18939)
                     /gene="hpnH"
                     /locus_tag="PK224_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HRB14168.1"
                     /translation="MAVPVSQMYTVTKYVLTQKLRGVKRYPLVLMLEPLFRCNLACAG
                     CGKIQYPDHVLDKRLTPAQCWAAADECAAPIISIPGGEPLIHPEMSEIVRGLVERQKY
                     VYLCTNAILMERKLDEYPPSKFLTFSVHMDGLRDEHDLAVCRDGVYDVAVKAIKAALK
                     RGHRVTTNTTLFDDANPERVRKFFDEMMGLGVEGMMISPGYSYQKAPDQQHFLKRSRT
                     TELFSKILGNRKRGWQFNQSPLFLEFLMGKREYQCTPWGNPTYNVFGWQRPCYLLQEG
                     YVSSFRELMEQTDWDSYGTGRNEKCADCMVHCGYEASAVEDTFGSFSGFAKTVKITLL
                     PNAR"
     gene            complement(19058..20284)
                     /locus_tag="PK224_00095"
     CDS             complement(19058..20284)
                     /locus_tag="PK224_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRB14169.1"
                     /translation="MSRVLDSPQVVMTPSRGHGANLTSREGMGPQDLLSVVSRRRGLV
                     ATIIAASLLGAFAVSALLLPKAYESTATLLPQLDSKEGGSLAALLATTGAGGMAQNLG
                     MGLPSMPTTPTDVFVAILKSRVMADEAIAKFGLMAVYHERTMHDTRVELAERVRVTVS
                     KEKVIKVTVEDADPQRAADIAAYFVSSLDRLNRTLNVSKASYNRAFIERRLAETQTGL
                     VKTEEALRDFQRNNKAVAVEAQSKAMIEAAAMIQGQITGYEVQLQVMSAYLSPDNPDL
                     SRVRSSIEELKKQLALLEFGKGAKGASPGDRLHPAMVTVPDLALQYGRLFRELKVQET
                     LYALLTSQYEQAKITEARDTPTVQVLDPPVPADKQIKPRVVFNTAVAGLLGACLAIIL
                     AYGLEARARRNAGLPG"
     gene            complement(20303..22936)
                     /locus_tag="PK224_00100"
     CDS             complement(20303..22936)
                     /locus_tag="PK224_00100"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRB14170.1"
                     /translation="MVVSMIPHHRPHPPVRQVALSHALIWMLVLSFILPPSLLAQNLP
                     PQVAPPGMMQQAAPGTPGSLFTPGGFNAPGAGGGFGQAIVTNPTALQPIVPTQTPCPI
                     PLSSDLTSKGTVPNLNDYWPVEPSSLLPSSIEQRMKQEQEERDRRQEKLQAEKEKSSI
                     EYQVQVEREKKGVSQLPFGQGVVPSLQQPAAQAQGQPGNQPGNSPGGVRPLPEKKAFT
                     AELLRQQDFNVEEAFAEFSVLHGVKSRVRQFGYDFFEAQANTFSPIQDIPVGPDFIVG
                     PQDSLAVHIWNVPDPSFNRSFIVPVERDGMIVIPQVGAIPVGGQTFSQVEQTVRSRLS
                     NLLKRFELHVSMARIRTIKVYVVGEVVRPGAYEISALATTSNALYAACGPARSGSLRQ
                     VKVMREGKTVAELDLYDFLLRGDRRFDQRLQSGDVVLVPPLGPVVAVSGSIKRPAIYE
                     VKPGVRLTELLTLAGGLTPLSDRQRCHLFRLDPERGRIMVDVDLVGALASQGHEKSRP
                     GVAGGDPVLLDGDYVRIGILPTQITNVVSLVGAVKSPGPYEYRPGMKVKDLLIHDQLT
                     MDAYADRAEIVRTDPVTYQTKVIQFSPRALLEGSEADNHVLQRLDQVVVASQHRPPNL
                     VLVEGELKRPGYFTIEMGERLSSVLKRAGGVTQNAFPAGLVLSRESVKLRQQAELERF
                     VASERQRLTAQAAGGAAGASGLSTAAVLSTGGGLAEQQVLSLRLQQLEAITSRLELGR
                     VVIRMDSIEQLEGTEDDIILEARDRILMPTPSQTVSIIGSVKNPSTVVYRPSLGLEDY
                     LRQAGGLTEDANKKEMYVMRANGTTDSAYLAVKELRSGDTIVVPQKIEARTPQLALWQ
                     TVASIIGSVALTAAGIAVVGR"
     gene            complement(23049..24626)
                     /locus_tag="PK224_00105"
     CDS             complement(23049..24626)
                     /locus_tag="PK224_00105"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HRB14171.1"
                     /translation="MNGTALASSNVPLHHWSYDAIERLIGLGVIDRALIGAKPFSRVQ
                     AAQYVARAVERVRADQVELDGREAIAEPLLERLLAEFRPELIRLGTVRARPDDKTGAL
                     RFGARVTSEFDASSIGGGQTVRFRENRGGEYSVNGVQNQTDVRAWAELSDWAAVMVQP
                     KFISNRHLLGLGATNNSENFYLREFSLKLSYANVALEVGRGTQWWGPGYHGSLLLTDH
                     AFPMEMIKLGTERPFQLPGFLSGLGDWKVNAFLGRLEENRDFPRAKLFGVRLSYLPAS
                     WLEFGLTRLTQFGGRGRDQSFPGVVFDAYISEPNQTGNRDVNEQAMADFRLRIPSIPY
                     LIPFPAGLQLYGEAGTEDKWSQLPVPSRTAFLGGLYIPQVFQGDTLDLRIEYADTDYG
                     RRRHPELRQVWYNNSPYTSGMRYRGFPLGHHMGTDGTDFFVRTTRYLSDTLQLGASFN
                     LQERDRGQPVHEKKREAAVDLTWWWSNRTQLTVGYTFQRLKNPGQVSQITPFVETFAA
                     GVEAQNHLFWTSLAMQF"
     gene            complement(24769..26739)
                     /locus_tag="PK224_00110"
     CDS             complement(24769..26739)
                     /locus_tag="PK224_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRB14172.1"
                     /translation="MPILFPTVFSAVQRRLLLFVVHVGLAALSNWLAFLLRFDGGIPA
                     QERELFATMLPLLLTVRALTFYPFRLYDGLWRYTSLWDLRNLALSIAVSSLTFAGVVR
                     FGMGVRAYPSSIFVIDALLLLCLLAGLRLFPRLIRETRWFGSGRKRVLIVGAGDAGAM
                     IVREMRNNPSYGYRPIGFVDDNPAKVGHRIHGVRVLGDRDCLSHIIARQAPDVVLVAM
                     PSAKPAAVRAVVKALEPFHLPIQTLPNLRDLLQCRVEVSQIRNLSIEDLLDRVPVDLD
                     PEPLRTLVQGERVLVTGAGGSIGAELCRQVAGLSPAALVLLDRYENGLFAVANELKEA
                     GQTVISPLVGDITDVGQMNRLFAEYRPTLVFHAAAHKHVPLMEGNPCEAVSNNVGGTR
                     IVAEAAHRHEVERFILISTDKAVNPVSVMGASKGVAELLMQRLARSSRTIFTTVRFGN
                     VLGSNGSVVPLFLEQIKAGGPVTITDPNMRRYFMLMTEAVHLVLQAARLARGGELFVL
                     EMGEQISVVDMARNLIRLSGLVPDKDIALTYLGCRPGEKLTEELVAGHETVESTAVQK
                     VLCIRPDPARNFAQLSGLVARLEESAAEGNVSKVLALLCAILPTYCPAGTLLSQERGG
                     ETDALLSEIPAQSPAGIETVAGLAPAAPHRRK"
     gene            complement(26885..27661)
                     /locus_tag="PK224_00115"
     CDS             complement(26885..27661)
                     /locus_tag="PK224_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB14173.1"
                     /translation="MSALDYSAVTEKSGDRVTQEALSMVYTRYRFAADFCRRRRVLEV
                     ACGPGVGLGYLARHAGEIVGGDLTGSLLGQARRSLRGVIPLVRLQAGALPLRAASRDV
                     IVCFEALYFFEDAEKFLRECRRVLVSQGILLLCMVNPEWPEFNPNSSAHRYYSARQVS
                     VLLKETGFHCNLYGAFPATTASWRAASVAGLKRAAVAWHLIPSTMDGKRLLKRLFLGP
                     LVPFPEAVEDGMAAYAQPVPLESNQPVSGYKVLFAVGRSA"
     gene            complement(27658..28770)
                     /locus_tag="PK224_00120"
     CDS             complement(27658..28770)
                     /locus_tag="PK224_00120"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB14174.1"
                     /translation="MAYKVPFIDPRSHYAKLKPEIDSAITGCLANGDLVNRHQLKDFE
                     QHLAEFVGVKYAVGVNSGYHALLFALLGAGVGPGHEVITVAHTFVATVSAIVHCGAHP
                     VLIDVGPDHNMDADLIERAITPKTKALLPVHLNGRVCEMDRMLAIADRHGLAVVEDAA
                     QALGATYDGRRAGSQGLAGCFSFYPFKVLGGFGDGGAITTNDPDLARMATRSRYNGED
                     RETGEYHCHGQTALLDNVQAAVLDVKLRHLPAWIAHRRRISELYRQGLSGIRELRLPH
                     FDQTRHHDIYQNYVIRTDVRDRLRAYLKEQGIETLVHWAKPMWEHRGLGLVTPSVPET
                     AALCREVLSLPMSAETTDDHVAITVACLQSFFSKRP"
     gene            complement(28788..29762)
                     /locus_tag="PK224_00125"
     CDS             complement(28788..29762)
                     /locus_tag="PK224_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB14175.1"
                     /translation="MRQCSYAQAIREAHAELLRHDPRVFVCGQGVWNPWYAGKSLLGL
                     DKEFGRERVFDCPVSENATTGAAIGAAIVGMRPIVFHARMDFMLLAADPIVNQAANWS
                     YLFGGQVSVPVVIRAVVNRGGEQGAQHSQSLQALFAHIPGLKVVMPATSYDAKGLLVA
                     AVRDGNPVLYLDDRWLYESEGDVPEGLYEVPIGSAAIRRSGKEVTIIATSYMASEALK
                     AAETLAQRGIDVELIDLRTIKPWDRDLVYSSVGKTGRLVIADAAWMSGGIAADIAAAV
                     AGDIFHALKAPIGRVCLPDVPAPTSAALERAYYVGAEDIVASVEKVLL"
     gene            complement(29770..30765)
                     /locus_tag="PK224_00130"
     CDS             complement(29770..30765)
                     /locus_tag="PK224_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRB14176.1"
                     /translation="MDATIQVGGAAAASRSLLRMMLLIRLVEERIGELVSAGEIKTPC
                     HLSIGQEAIAAGVCAALSRQDTVWGGHRSHGHYLAKGGDLPEMMAEVFGRVTGCSKGR
                     GGSMHLVAPAQGLYGTVPLVGATIPLAVGAGLASKLRHEPHVAVAFFGDGATEEGHFH
                     ESLNLAALYRLPVLFVCENNFYSTHMPLSARRLKDNIVQAADAAGMPGIRLDGNDVLA
                     VLDGTRQAVAQARAGAGPCLLECRTYRWRGHVGPAWDLEVGERRRSELDEWMPKDPIL
                     RLRRRLIEEGQSTNELDALEPAIRGEIEAAVTFARESPLPDDSDLFRHVYAPRRT"
     gene            complement(30770..31399)
                     /locus_tag="PK224_00135"
     CDS             complement(30770..31399)
                     /locus_tag="PK224_00135"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRB14177.1"
                     /translation="MKRFFDLVVASGALLLLSPLLLVLAMLVKLGDGGAVLYRGPRVG
                     LGGRSFRMCKFRTMLERADALGGPSTPADDPRVTPIGRWLRRYKLDELPQLFNVCIGD
                     MSLVGPRPEVQQYVDLYTDEECAILAVRPGLTDWASLWNIDEGALLQGSPDPDKTYLE
                     TIRPVKIRLQLEYVRRRSFRTDLQILRHTLVALVGGKRSHGVMFVPEKG"
     gene            complement(31431..32567)
                     /locus_tag="PK224_00140"
     CDS             complement(31431..32567)
                     /locus_tag="PK224_00140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB14178.1"
                     /translation="MEPGYRLPFVTVLVPCRNEADFIERCLTSILSNGYPADRLGVLV
                     VDGRSEDGTRSVLGAYAQRHACVRVIDNPGRTTPKALNLGVREAQGDVVLRVDAHARL
                     ERGYIRRCVEALYEHHADVACGLMRTVPAVDSPVGQAIAAALSHPFGVGNSYFRIHVS
                     DATWVDTVFCGCYRREVFARVPAPEGSRASGVMPLAPASIDRQGPFNVDLIRGQDMDF
                     SLRLRKTGGRMLLVPDVASEYYARSTISAFWRQNWNNGLWAMLPFAYTDGVPVSLRHL
                     IPLFFVTALLATAAVGVVSRPWCWVSGVIPAVYGMVNVAASVHVAWTSKSFSRLILMP
                     FVFAALHLGYGLGSLWGLVRLISLPRFWRKLRLAGAGGASAVEL"
     gene            complement(32574..34001)
                     /locus_tag="PK224_00145"
     CDS             complement(32574..34001)
                     /locus_tag="PK224_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14179.1"
                     /translation="MEFYLVLAASTVGIAGLAALLYARTVSLSFPLGIAFLYFWSLYG
                     GWSIVADGLGADSQQQHHYLYWKVFPVELNEEYLWTLLLYALFVLVIELTLLAVVRSD
                     RPHMDQAAEALWISHTVLLVIAALAAGTSFLIVKDQLATAVLLGKSGYSVTRGGLGEM
                     VPLFTLHQVLNRVALFSLAIGIGVWFTGRQGRWLAGNATLVTGAGYSVLLGGMFLYVT
                     ALGNKNELFSALILGGFFHLANTRRVKWGFVGAGAGMMFMGIGLVDFLRAFPLIDLWE
                     GGAWWDAIQWVPEIHASNEAFGAHLSLYGALYFHSPLTYGSSLTALLFSVVPRILWPE
                     RPGDIYSHYASSVGIVEGVGEQGFSIHHATGWYLNFGLPGLLFGAVLWGWIWGRCFNA
                     YLCAEATRDRGGRWRYAFAVMAPWGFVAYIPPLIRAGLEGYKGLVIEGLLVPMLVVSI
                     ASFGSGLLARLFAPLSMAAAPAPTE"
     gene            complement(34014..35852)
                     /locus_tag="PK224_00150"
     CDS             complement(34014..35852)
                     /locus_tag="PK224_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRB14180.1"
                     /translation="MLILGINEGIDASVVLCRDGRVVFALQEERVSREKGAIGFPLQA
                     VMACVKAYGLDARRLDHVCLSNLNSPVKETREDLLRYYARSSRPWKDLVLGGDWSGAA
                     SRCAGLVPASIEDLGRRWQVSRRTATSHPVIERQLAQCGLDGIPVSRFHHHSNHAASA
                     YYALRKNWEEPHLVLTLDGGGDNTCAQVYLAQHGELRLLASTPTGHSVGNIYASVTYL
                     LGMRPHEHEYKVMGLAPYAGGERGREVANSFARYLDLDPQNPLCFRRKTLERTSAILP
                     RLMDDLRAVRFDLMAAGVQLFTEDLMCRWVAAAMERTGVRKVLAAGGVFMNVKANHRI
                     AELPALEYFDVFPSCGDETLPFGAVWQCHLRGGGALDDIHFDNIYVGPEAGAGLAEAR
                     ARYGEAAEFQELDDPEERTAELLAQGHIVARCSGRMEFGARALGNRSILADPGNPGVV
                     NVINRMIKQRDFWMPFAPAVLGEQAERYVRIPSALPRPRVSPYMMHAVETTWHRADFI
                     AAVHPADHTARVQTVWKDLNPSFHALIEAFGRMTGRYVLLNTSFNLHGFPIVAGACDA
                     VEVLLRSGLDYLVIDRWLVTKRGRLEPVPASSTQQGREAQNPISPD"
     gene            complement(35846..36901)
                     /locus_tag="PK224_00155"
     CDS             complement(35846..36901)
                     /locus_tag="PK224_00155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB14181.1"
                     /translation="MSDMVGMAVSYEDRQRDSAPLQGRPIRVLYLFPGEAEGSAMIFA
                     KKQVEAVREAGVAAETFCLASRTDLRSLRREYARLRGLMDSFRPDLVHAQYGTMTAFF
                     ASLITPVPLVITFRGSDLNPAPSDPWLRSALGRWLSQYAARKAARIICVSEGLARRLW
                     WARDRAVVLPSGVDTNLFVPRSRSDARQELGWAEEDRIVLFNAGLSPVVKRLDLAQAA
                     VNEAVKECGPIRFVTLDGHVPHSTVATMLNGADCLLLTSDWEGSPTIVQEALACNLPV
                     LSVGVGDVRERLAGVVPSRIVERNPQALGKGLAEMLARPVRSNGRAWTAPVAQDHIVR
                     KTVDLYREVLGSKRAAC"
     gene            complement(36913..38061)
                     /locus_tag="PK224_00160"
     CDS             complement(36913..38061)
                     /locus_tag="PK224_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB14182.1"
                     /translation="MTSVHPPRRGRRRVVLALASMLVSLVVAALFLEVICRWLPVNEG
                     LRGLSVDEQNPVFRFTSHRESVYSRGWNFSIVNRVRTNNYGFVNDQEYDPSAKTPLLA
                     VIGDSFVEAVMVPFPQTGVARLAGTVGSAGRVYGFGTSGSSLSQYLAYAQYVRDTFRP
                     DGLTVVIIGNDFDESLRKYKSEPGLHYFAESPDGRLDLVRIDRPVPWWRDFLAGSSLG
                     MYLYVNLEIREVWKRWAYRPGQSAISRPRFVGNTAVEASPARVADSDRAIEAFLQRLP
                     EMSGLPPGRIALAVDGIRPELYDTDRLASVGDVYAAMMRMHFMDRARQQGFEVIDMQP
                     AFIADYRQRQLTFEFPDDNHWNATGHEVFARAVQQSKVYRETFRSVGP"
     gene            complement(38150..39514)
                     /locus_tag="PK224_00165"
     CDS             complement(38150..39514)
                     /locus_tag="PK224_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRB14183.1"
                     /translation="MGRNALGLGMGLSLLTAAQLLSSFGIQWYTVAHLGAGPDTDALY
                     AGTTLLQLLSVVVMDPLSFVLVPLLSARPEDERRRLAWPLFLGVGALVALVTLVLYYA
                     APYLVRVMVPGFSEATTHLTVDLTRIQLTALIGVACFTLLSAFYQARDRFIWTAGALL
                     LCSLLGWWVLVKALSSGGVRLAAWVQVFVLSGPALLLLKALGAWVPGSFPGAITCLRE
                     VGRRMRPLVLSAAFVRTGFVADRFLTSFLGAGSLVMLELTWRVLAAVVRIFNQGFVTP
                     VVPTLAKLAQARAWEEFVALCRARLVGVAGLSALAFVGLSTVAIFGQSIGLWSGGQPG
                     QGGITSQEMATLRMTFFACAGGLLCGGVNHLLVNAFYAEGETSIPARVEAVASLGGLA
                     LKALGFLAGGLLGIAVAISLQYALAGMLLATAFYRRMLPRLRECSSGPVGRCVSGEPS
                     NRSR"
     gene            39675..41945
                     /locus_tag="PK224_00170"
     CDS             39675..41945
                     /locus_tag="PK224_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14184.1"
                     /translation="MLQVSSFTWRALTLVVICTPLLLLHARMASADSPTSSASASHDL
                     DLRLETTTYVLNLEKQGLEDNTFTNGLGRDTTLIGNLLNVYVRKRLFASMDLDLGLFA
                     NMPFGHDTEVSQVRPIIRLQYRPTDEITTVLGTLAGPHRDFHDAVFDNANRFVRPLEQ
                     GAQVLVNSRYYRQDLFINWEQAFRGSATNRYDVGYAGQLRAGPFRFNGQAHWVHNGQA
                     LLKLDRSFNTADNLVTALGPELVVEPSTYFPALTWWRQAGIRATYLTSLNEPLAGGPA
                     IRGRGYELSVWMDFSGWRPSVSFWKGRHFLSQQGDPEYAASNFPEFGLAKIIPLGESA
                     SIEFGVQARRIRAFFTEDGITYQNRDTFKWVSQQYLVFNWNWDTTDGQFVRDLFSAGD
                     TPSSEPKPTHLPSRFTAKFDALTYVYNIGYSGLRQIGGRPVANSTLAGQYLAPVLRYS
                     PSSRLNLDIGVFAGLPVGDTQRFHTVQPILSAEYELWPAVSLIAGTIKRNHPLVDALF
                     NDASLFSRPLEQGFQLLVNREHYQQDLFINWNQIETFQKAERFDVGYAGRASAGFFSL
                     NGQVYWTHSGGAQYSESRTFFGVGLPRDRPASNNFLTAVGPQFTFQPNRYSSALSWFR
                     EIDVMALYLTSQNEPTQSGAPIVRGRGYQLTAGVDLDGWRPYVTIWRGEHFVNEQGDP
                     AYFAGNFTEFGLLRDVLLPAGFSLRVGGFGRVIEDRVTHTEYALLNWSWDQSPWRGFC
                     LRPTLLHRSESSCSRD"
     gene            42034..42663
                     /locus_tag="PK224_00175"
     CDS             42034..42663
                     /locus_tag="PK224_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB14185.1"
                     /translation="MFRRWIGTVLGIVVMYAPALVHAENYAAFAVGPNVATLDTTDAG
                     KLDLNTNLAFGAKYGHYFDDRGFNWFGLEVDVYRSQSNVKAQTPPRQGTNLLLSGPIP
                     GSDFLVHSLAFNALVRVTGYQYKVEPYAGLGLGLNLGNISDGNFRPEASFAPSFNVLA
                     GIKYYLTAKVAPFIEYKYNFAHFSFDRTNVTADYRANLFMFGVAYHFDR"
     gene            42765..43661
                     /locus_tag="PK224_00180"
     CDS             42765..43661
                     /locus_tag="PK224_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB14186.1"
                     /translation="MTNIPNACYLSLDVEDYTHATMLDMGLRPRTNPEQTWRGLRRIL
                     SVLRQAGGHPNMTLFTTGQIARDQPDLIREMSRLGHEIACHGDQHENVCTLSRTEFAG
                     NVRRAKDALEDACGQRVIGFRAPNFSLDERSTWAYAVLSEAGFLYDSSLVMEAPRVSR
                     QPYDVFELSGARLYEFPLYRYPVVGTRGIRVIGGTYFRCLPLSAIIALMRQALRLGYT
                     PLVYLHAADADDQPSPVTWKDMQGLSYLSRSRWTIRQKQWMLGTHAVADKLLALLKLF
                     PHLGPMSTTLPMKDNSRLNASD"
     gene            43693..45450
                     /locus_tag="PK224_00185"
     CDS             43693..45450
                     /locus_tag="PK224_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRB14187.1"
                     /translation="MYILGLATMGESAAALMKDGVLVAAVEEERFTRVKHEGCFPLRA
                     ITSCLNRAGISLAEVAHIGVYWQPLRMGVRTKAVLKTALTHPTAFLGKMGRALQEFTP
                     AGGGQAVASQGSWAELFLVRSLLRKHFGAFKARLHYLDHHRCHLASAFFASPFEEATL
                     LVFDGAGEDVSTTLAVGAGTDLRVLRSIPWPHSLGHFYSAMTGFLGFAMLDGEYKMMG
                     LAPYGSPDYLPFIRQNILLTDAPGSYRLNGDLLDYHAALKKQFHPKLLEAFGSPRLSD
                     DAPFTERHRQIAASAQAAFEEVVGDMASWAFQQGGKRTNVAIAGGCGLNCSANGKLLR
                     DGPFTRMYVPPAPHDAGCAVGAALLLHHERLGKPRQFEMTHAYYGPAYGEQDIGRALC
                     ALGLEARPMADEEALLQRAVQALCSGQILAWFQGPMEFGPRALGSRSFLADPRHDSIR
                     DTINGKIKKRELFRPFAPSVKEEAASKYFVLSQPSPFMTLAVPVRAHQRARIPAVTHI
                     DGSARVQTVNRASNPRYWNLLDRFEKETGIPVLLNTSFNIQEPIVCTPEQALATFVRS
                     GVDALVIGNYFIDRAMLPA"
     gene            45921..46409
                     /locus_tag="PK224_00190"
     CDS             45921..46409
                     /locus_tag="PK224_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14188.1"
                     /translation="MNGRNVAHSSLWVLLISGFFGCAGAGPFVTLPSSAFFETDVKER
                     EALQVVSRTQEARRANCQQSASCEEVTYLRGLVALFENREDAIHAFQELRTTVPNSRY
                     AASSTRWLTLLQGGQPASVRHEPLFMQLRQEILHGLLDPDTLAASRKLKEQERRIAEV
                     SR"
     gene            complement(46722..48467)
                     /locus_tag="PK224_00195"
     CDS             complement(46722..48467)
                     /locus_tag="PK224_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRB14189.1"
                     /translation="MAPEPIFFRDLAYVFIAAVLGGASAYLLRQPLILGYVFGGILIG
                     PFTPGPSISDVHSFELFAEIGVILLMFCIGIEFSLNDLLRVRWVALLGGPLGIFLSIL
                     LAVGLGHVVGWSWIQSVVIGSVVSVASTMVLARLLLDSGDLRAKHGRVMIGITLVEDL
                     AVVVLTVLLPALGALEPGRFATIGKALWLAILILVPFAYLAAKAIPPLLTRIAKTQNA
                     ELFLLVALSIGIGTAAVTQMAGLSLALGAFLAGLIISGSEYGHETLARLLSLRDAFVA
                     LFFVTIGALIDPRVVWANLPLLGLMLGLIIGGKFVIWTLVVRLFRYAWGTALLVGAGL
                     TQIGEFSFVLVQVAKTAGHVSEDVYNATLAASLLSILLNAPLVRYAPGWIATLHFKMG
                     RASLPIQGGVPEDQAGHVVLCGFGRMGSAVGLALDHFELPYTVIERDPDIIRQLRRRR
                     ISCVYGDASQTELLKAAGAQHAALAIVALPVIHETSLTVRRFRGLNEKIPLLARAHGF
                     REAEDLKDVGATEVILPEVEGAHTLIRHAFQALKISKSSILDYLKSCQAFRSSGEGLE
                     SGNVEAGKAGAGRST"
     gene            complement(49426..49911)
                     /locus_tag="PK224_00200"
     CDS             complement(49426..49911)
                     /locus_tag="PK224_00200"
                     /inference="COORDINATES: protein motif:HMM:NF022841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3192 domain-containing protein"
                     /protein_id="HRB14190.1"
                     /translation="MDKLHGYAGDKMALYKTTTILLVAMPLLLCTSCMPRELRSIDNA
                     ISEYNEKSAGIKLGDSKEKVLAILIPTQAGLSKQASKRPESFVTDNLLTEIFFFRSSR
                     QADGLTTDDEFTPYVFKNGALVAIGWTSLGGPKSVAHPIPTNPNRPVHCFQNGANIDC
                     H"
     gene            complement(49918..50436)
                     /locus_tag="PK224_00205"
     CDS             complement(49918..50436)
                     /locus_tag="PK224_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14191.1"
                     /translation="MAENIEQILEADPLGNLARKERKALLISSAVAFAMAKAGIVPSQ
                     ISFLTIEVTEINEIELLRLIIMVVSYFLLSFVLHALPDFLRWKVQIAEHDLRGARHRW
                     EYEEKYGSDYDEPTPDWKDPPDYYEKEWKSFSMRRSWYLNLTQLKALVLDVILPVVIG
                     MAAIMVVLKAIF"
     gene            complement(51188..51901)
                     /locus_tag="PK224_00210"
     CDS             complement(51188..51901)
                     /locus_tag="PK224_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14192.1"
                     /translation="MSRWKEFRREPIAGEWTRKQKRGYHRVRSLLWFWECHQFQVLWV
                     TLSTAEGGDAEKLTYHHKQLRQRIERQLGFQGLEYYQVRTEEGHGVLHIFWAWRVPDG
                     ERARRFWISQEWLSSQWQALHGAPVVWIKAYQPSHRSRNRLSRYVISQYVQDQCGYVN
                     MCWSWKRSLGFPISRLWEEMRNQWVSRNVYRRIRGEIEIPRIVFLKTWEDLLSGHPIW
                     FSGTILQLVLGKGLVYQEV"
     gene            complement(51898..52104)
                     /locus_tag="PK224_00215"
     CDS             complement(51898..52104)
                     /locus_tag="PK224_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB14193.1"
                     /translation="MVSTKLITIREAAERLGLKESTIRKYILKRQIAYVKPSVRAVRI
                     PIEELERILAVGLRPAIPQAEGAR"
     gene            52304..53017
                     /locus_tag="PK224_00220"
     CDS             52304..53017
                     /locus_tag="PK224_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14194.1"
                     /translation="MPIDRVAIHDQFHGEMKKARERILTTADGEVGWLLKFIQTDLDT
                     LTASEWMVLAFEIASFVDDVANRRGAEIATEAGWSVRSIPGDGFRGTLPSRGEAKEIQ
                     AMVLGSLEKLWETAVAAFTFPQFTIIVTLPVEDERKGSVFVATKRKVKEFEYRFAHLL
                     VDYAGRIRRCPECQRIYLAIRFDQRYCGPRCQTRVATRKWRETHLPKKPKALADTTRT
                     KADPTRKRRNGGSDGKTKR"
     gene            complement(54143..54218)
                     /locus_tag="PK224_00225"
     tRNA            complement(54143..54218)
                     /locus_tag="PK224_00225"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54183..54185),aa:Cys,seq:gca)
     gene            complement(54324..55529)
                     /gene="ispG"
                     /locus_tag="PK224_00230"
                     /gene_synonym="gcpE"
     CDS             complement(54324..55529)
                     /gene="ispG"
                     /locus_tag="PK224_00230"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRB14195.1"
                     /translation="MHITRKKTRQIQAGSVKIGGDAPISVQSMCSTDTRDVNATVAQI
                     RQLEEAGCEIIRVAVPDEAAAAALPQIKAAMTVPLIADIHFDHRLALKAAEVVDCVRI
                     NPGNIGAWWKVQEVIKAVNERGIPLRVGVNGGSLERPLLDKYGWPSPEALSESALNAV
                     HALEDEGFTNMKVSLKASDVHHAIDAYYLFSTQSNYPLHIGITEAGTAMTGAVKSAIG
                     LGWLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLGHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLMRKV
                     PLEELMDTLIHEVELLAKEKEAEAAGGTHETAASSDGQAEPGWELIGHAPDEHSTIVR
                     DIPVLPTKR"
     gene            complement(55574..57520)
                     /gene="dxs"
                     /locus_tag="PK224_00235"
     CDS             complement(55574..57520)
                     /gene="dxs"
                     /locus_tag="PK224_00235"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRB14196.1"
                     /translation="MSLLKNIHSPTDLKRLSPEQFPELCQEIREQILTVVSNVGGHLA
                     SNLGVVELTVALQYLLDTPKDKIVWDTSNQAYTHKLLTGRREQFHTLRQYGGLSGFCK
                     REESVYDTFNAGHAGTGVSAAFGMVEAREQRNEKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGTNKDFIVVLNDNQMSISKNVGAISAYLNRTFTGEFYARMREETGQLLRKIPHIGLE
                     VQKIARRAEELAKGAILPGLLFEELGFQYAGPIDGHNFEHLLPTLENVLKMKGPVLLH
                     VITKKGLGYQAAMDNPVWFHACPPFVRETGVPAKKASRPSYTSMAIDALIKVAHQDKR
                     IVAVTAAMCEGTGLNAFEKVFPDRIYDVGIAEQHAVTFAAGMAAQGMKPVVALYSTFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDFAFLRHVPNMVVAAPKDE
                     NELQHMMKTCVTHDGPASVRYARGVSLGVPMDPEPTALPIGKGELLREGTDVAIVAIG
                     VTVWPAMKAAERLAQEGISAAVVNARFVKPLDTELIIKTAKNVRCLVTVEEGCKMGGF
                     GSAVLEALSEEGITNLRTKMIGLPDWYIEQGPQDLLRERYGLTADGIYNSVKALFGAS
                     VATDDAARLASLVGSLPHGDEQGS"
     gene            complement(57703..58701)
                     /locus_tag="PK224_00240"
     CDS             complement(57703..58701)
                     /locus_tag="PK224_00240"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HRB14197.1"
                     /translation="MRYTIHCFCLLALLSVLPPAAGAEGFGPFPVRNFQALDQLVLAM
                     PGERATVLRKGDFDVRLEAANTASIARDSEAQAEVAMKFETIRAGLFLRYGLTDRLEL
                     GAEIPGYHRYRGFMEGAIIGVERGTTGVAPPRKALRETGYAFNISNGSRTLFQGSEGA
                     TGLGDISFYGKYQILKETSSLPALSVRVGVKAPTGDTGEVFGSGHPDAGIGLALDKKF
                     AGNWILYANLNGVFPTGRIAGLGLQPVVSGLVAVEYLWTENFSITVQFDYYSPPFHGT
                     GTRVLDKGVTESVLGVSYRILPGLLWQLYGVENLDFITGSAADFTLSTVLTYRFRS"
     gene            complement(58732..59937)
                     /locus_tag="PK224_00245"
     CDS             complement(58732..59937)
                     /locus_tag="PK224_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRB14198.1"
                     /translation="MQCHYLSSIRSCLALAGLLLLSLPAPAGADTQIFPVPSVSTSRN
                     DGNDAGLIAPILIADPDGELKYLMAPMLIQNSIVGTRGVFNLFKYDPGGRQMRFIASL
                     TERIERKVLFDYVDPAFGNGQYSLNFGGTFFKNATSRFFGLGQATTQAAESNYTAREA
                     RAYWRLGLYANEVTQISVGQRVRQVQLQRGAIDLPFSVEQFPTVDGIQGESIIVGHRA
                     SFYYDTRDSLVSPTDGVAITAYAELNQNVKNGDHPVYSRYELEVKKLFPSESKRAILV
                     VRADLQATIGSQVPFFEQSSLGGQNNLRGFGLDRYIDKHLIAFSIEERIHILRTKLAG
                     VTADFEVAPFLDTGQVFNSFKDVSFQDYRMTPGLGFRAIVRPNVVGRVDYGYSREGGA
                     VFAGLDFPY"
     gene            complement(59972..60586)
                     /locus_tag="PK224_00250"
     CDS             complement(59972..60586)
                     /locus_tag="PK224_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB14199.1"
                     /translation="MHTSRWMMIGALLALQIVVGGLSSAMAGPATDSVKGTIDEVLRI
                     LNDKELKAPARLEDRRQRLEKVVAQRFDYPEMSRRSLGAQWNQLSDKDKQEFIDLFRT
                     LLTNTYADRVETYSGEGVQYLNERTEKEYAEVRTKVLSGKTEIPMDYRLLNKSNDWHV
                     YDVVVDGVSLVNNYRGQFTKILHTSSYPELVDQLRKKSDKIKAP"
     gene            complement(60674..61117)
                     /gene="mlaD"
                     /locus_tag="PK224_00255"
     CDS             complement(60674..61117)
                     /gene="mlaD"
                     /locus_tag="PK224_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRB14200.1"
                     /translation="MERAKLELMVGIFVLVGIACLGYLSIKLGKLEVIGGHNYPVEAE
                     FTSASGLKPGASVEIAGVEVGRVRNIGLSSDRALVALAIQDGVKLYSDTIASIKTRGI
                     IGDKYLALSVGGGGDPLKPGDKIRDTESGLDLEELVSQYVHGKVN"
     gene            complement(61129..61890)
                     /locus_tag="PK224_00260"
     CDS             complement(61129..61890)
                     /locus_tag="PK224_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB14201.1"
                     /translation="MIKLVGVEKTLGKQPVLRGVDLTIPTGKLTTIIGRSGEGKSVLL
                     KHIIGLMQPDRGEVWIDGTNIARLKGQALNEVRKKFAMLFQGAALFDSMTVFENVAFP
                     LREKLRLKGEIVTRRVEEKLEQVGLKGMGHKFPAELSGGMRKRAGLARALVMEPEIIL
                     FDEPTTGLDPLMAKAIHDLIVAMQQQFKFTAVMVSHEIPEIFGISDYVAMLKNGRIAE
                     MAPSNEFVKTTDEEIREFIFVAGTVTPKGLPTASL"
     gene            complement(61966..62733)
                     /locus_tag="PK224_00265"
     CDS             complement(61966..62733)
                     /locus_tag="PK224_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB14202.1"
                     /translation="MEGIARIGRYTIDLTEQMGRMMLFVLSSFAWLTRPPFRVYQIVK
                     QLNFIGYKSTFVVVLTAVFTGMVLALQGHYTLRKFGSEAVLGSAVALSIIRELGPVLA
                     ALMVTARAGSAMTAEIGIMRITEQIDALDTMAINPLQYLIGPKLVASLIAVPLLVALF
                     DVVGIYGGYVVGVQLLNGNEGAYWSSIESAVEWKDVYGGILKSISFGLLISWVCCYKG
                     FHTRHSAEGLGTATTEAVVLSAVLILVWDYFLTSVLL"
     gene            complement(62794..63549)
                     /locus_tag="PK224_00270"
     CDS             complement(62794..63549)
                     /locus_tag="PK224_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14203.1"
                     /translation="MTAIGVFVATRWESAAVRQAFAASEMRVAGGIRCAVARQGQVEW
                     WVIPMGVGPERATTTAGRVLGEQSFAAVWSTGFACALGQAVIGEVLIGTHVTMEGGAE
                     DGRSVPCAAAGADWVRQAVQQQRLPVQAGQFVSVPRVLCRAEDKQEIATRAGGVGLDM
                     ESAALGFVASERKIPFVIVRTASDLVDESLPLDFNLFLRPSGWVKGVAACLAHPTSLI
                     GLNRLRVQSRIAGTQLTAVFRACADKALREGLA"
     gene            complement(63604..65781)
                     /gene="shc"
                     /locus_tag="PK224_00275"
     CDS             complement(63604..65781)
                     /gene="shc"
                     /locus_tag="PK224_00275"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HRB14204.1"
                     /translation="MRLLKNLFNRLSDSLLVSVPSRIRAARDFASAPVLRLVSNKSAV
                     PASGDSAAKRAPAHSTGQVEALEEALRKSQAWFLARQDASEGYWVAELEADTTLTSEY
                     LMLRRFLDCIDPERERKAVRYLKSAQLPDGGWSIYHGGPAEISASVKAYFALKLSGVS
                     ADEPFMVNARTCILDKGGVVAANVFTKIALALFGQYDWRGVPSMPPEIMLLPKRFYFS
                     IYAISYWSRAVLIPLLIIFAKRPLCHVPPELGIDELYTEPPAEIDYGMVPPFKKDRTW
                     FTARNFFINLDALLKIYDRSPVEWVRQKALKCAENWMLDHMKGSGGLGAIYPAMANSV
                     MALHCLGYKNDDPLMVKAIREIEELEVHDTVQDNGQCVDAMHLQPCHSPIWDTALLIN
                     ALIEAGMPQDHPALQKASTWLLSKQTKTVGDWIISAPGAEPGGWYFQFENELFPDVDD
                     SAVVLMALAKVHLSDEAQQRLAIRRGCRWVTSMQGSDGGWGAYDVDNNRIVFNYIPFA
                     DHRALLDPSTADLAGRCLEMLATLGYDWTHPAVASALTFVKNDQEQDGSWYGRWGVNY
                     IYGTWSVLSGLRAIGEDLSSPYIRRAVSWVESKQNPDGGWGESCLSYGDVSQSGRGDS
                     TPSQTAWALLALMAGGVTDSFSLARGIHYLIRNQRKDGSWEEVRHTGTGFPRVFYLRY
                     HWYCQYFPLWALAMYRNLKTRGTTRADELRLQAYQSGQFRSPR"
     gene            complement(66004..69684)
                     /gene="smc"
                     /locus_tag="PK224_00280"
     CDS             complement(66004..69684)
                     /gene="smc"
                     /locus_tag="PK224_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRB14205.1"
                     /translation="MYLKSLEMLGFKSFAEAKIQFPKGITAIVGPNGSGKSNVVDSIL
                     WVLGEQSTKTLRSEKMEDVIFNGTEVRKPLGLVEVSLVIGGLGELRLDAISGLPSELS
                     EYQELMITRRLYRNGDSEYLINKTPCRLKDIRNVLIETRAGSKGHTVIEQGRIEQILQ
                     ASPQDRRELIEETAGIVRYKKQKAEALRKLEATQQNLVRVRDIVAEVKKQLNSLERQA
                     RQARSYQTLQQEARGLEIELLSRDYRTMHADLESVDHEAREVEAQEAEQVAEQARLDS
                     EQEAIRLRMNDAAEAISRVRDTLAGTEQRQSQALTAAEVERNRTELFERQRMQAAQEM
                     ERLAADQEQAQSEIETLRAMLLQLEGDIVEQEAQFQQADVEAKSLSGHRASAVAEEER
                     ARKDVLNLAVLVANTEQSLTQVTARQQETAARAERVSREQEQLVAQVAGLDQQRDLLA
                     AQREEASGRIQELMAERQAAIERIEGLGGQITGLDRDIVKFSEDVAGVESRLGALQGV
                     VREEMGYGREGEEEGTALKTCDGVREALAEWLVIPPGLDRAVETILGERVRGWLVDEP
                     SAACRAVEFLKGKELGRGAFLPQQLRWAPEQAALDSSASWWPTLTGQAGVVGRATDLI
                     RAEGASAATLSYLFDGIVFVESLDVALQLWQQHQWAAPAGPTYVTLSGETLDAAGVMT
                     GGGSSASGGLLQRRREVLELEARRTAAVQALEQARSAREDAAAELGLGREDEQRLSRA
                     IREAEMLELSLQKDDAGVERQRGELHRRMEVLAEELQRGFAEQARLQEEFQSSQTQLG
                     QWVAEKVGQETGLLHVRERLVVIESQSLAIQQRLTEVRLSLESMRGRREHATNDIARF
                     TQRLDAAQRRATDLEEQVAGLVEATENSREEQTRQEALCRELGAEVDGIKAELVAAQE
                     RQAQEMAGLHAVEETLSQVRQSLSALHDRRMRAEVRKAEVKAHLSTIESTLAGTYQIE
                     PATLLLPGSDQPVPEGEEPPAPVSILETPQLREQIQKIRERLDRMGAINLAAIDEHRE
                     LEERYQFLTTQEQDLSTSIASLKEIIQRINRTTKDMFVETFNELQQKFRDVFSQFFPG
                     GRAELQLVEEPLEEGVEDNGAREPGVEIVAQPPGKRLKSITMLSGGEKTLTAMALLIA
                     SFLIRPTPFCILDEIDAPLDEENIGRFTSVLRSLSSTAQFMVITHNKRTMAMADSLFG
                     VTMEEPGVSTLISVKLGDLQPA"
     gene            complement(69874..70836)
                     /gene="ispH"
                     /locus_tag="PK224_00285"
                     /gene_synonym="lytB"
     CDS             complement(69874..70836)
                     /gene="ispH"
                     /locus_tag="PK224_00285"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRB14206.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRHKGAVFVEELNEVPEGSVVIFSAHGVAKSVWEEAQSRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYELILIGHAGHPEVIGTLGQIPDKFHLVSSVKDVESLHVEKTQNLSY
                     VTQTTLSVDECRDIVEALHRRFPNIKGPHQEDICYATQNRQNAVKSLSKLVDVILVIG
                     SPNSSNSNRLRELGEHCGIPSYLIDAASDINPDWLKDAKSVGLSAGASAPEVLVTEVV
                     AYLKRLGSSEEVEELTVIEEDVEFLLPKELVTIESASRVSASAN"
     gene            71010..71726
                     /locus_tag="PK224_00290"
     CDS             71010..71726
                     /locus_tag="PK224_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB14207.1"
                     /translation="MPPPATTHKKILIVEDEKDILQLVKLYLEKEGFRTVSAMTGTEG
                     LRQVKAEHPDLIILDLMLPELDGLEVCKRIRLNQETALLPILMLTAKAEESDTVIGLE
                     LGADDYVTKPFSPKALVARVKALLRRLERNANIPQTQYHYGPLTVDLSRHEVRLDGQE
                     VALTAKEFGLLEHLLRNIGRVLTRDVLLSAVWGYDYYGTTRTVDVHVRRLKQKLPLLD
                     DAIISIKSLGYKLRESDVPA"
     gene            71723..73582
                     /locus_tag="PK224_00295"
     CDS             71723..73582
                     /locus_tag="PK224_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14208.1"
                     /translation="MNLGIRWKVALGTLAAVFVGLAVAGWLVIRSVEQTELSRMAETL
                     ETSSGLAAMTLRPLFDQSGSTVPGPLLHATVRDLSQQAHLRITVIRQDGTVVADSAVP
                     TEGLTHVENHLSRPEVAQALRSGRGMDIRASQTTGERTYYVARLLSASTPAQPGISVI
                     RLGLPLTSIDERVRHIQQDLATAFGAAFLLAMTLSLWVSRNLTKPLSEMAAAARQLAG
                     GTPGVRLVVSSTDEVGLLAQTLNQMTDQLETKIKEVSDDRAQLLAMLIAMVEGVMVLD
                     YRGTVVQVNPALERMFSLELTESRGRHYAELIRHEGLTALVSSVLQTRSGQGGEITLS
                     PSGRCLRVEASIAGGSREQEACAVFVFHDITELRRLEKIRKDFVANVSHELRTPLTSI
                     KGYVEALLDGGKDEPATATAFLEIIMRQSNRLNLILDDLLQLSQIESGQVLFRREPVD
                     LRALLERTVAVIKPLADKKHHTIELSLPDEYWVVEGDEERLVQVFINLLENAVKYTPD
                     QGRISMAIRNATHLRTTPPRAMIEIVVADSGIGIPEADRPRVFERFYRVDKARSRELG
                     GTGLGLAIVKHIVEAHSGQVWVEGNTPRGSRFVVHLPVAQESSTAVSTETGSR"
     gene            complement(73548..74540)
                     /locus_tag="PK224_00300"
     CDS             complement(73548..74540)
                     /locus_tag="PK224_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRB14209.1"
                     /translation="MAELSGLLLVVVILALLFDFSNGWHDSANAIATVVSTRVVSPST
                     AVLVAGVLNVAGAFMSTAVAKMVGTGIVDPQSVTQAVVASALAGAIVWNFVTLLLGLP
                     TSSSHALIGGLVGAALTHGGTAAVKFSGLRSVLEAMVLSPFFGFAIGLVLMVILSWVF
                     FRVPRALALRLFSRMQLVSASFMAFSHGANDAQKAMGIITLALVSAGVLPTAEVPTWV
                     IGSCALAMGLGTAVGGWRIVRTLGMRIVKLEPVHGFAAETGAAIVLLATAHIGLPVST
                     THTITSTVMGVGAVKRLSAVRWGVTRRILSAWLFTLPGAALLSTICYLFLSKLQ"
     gene            complement(74605..75222)
                     /locus_tag="PK224_00305"
     CDS             complement(74605..75222)
                     /locus_tag="PK224_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRB14210.1"
                     /translation="MFGLIPREEAFFDLFKNAAHNMIEGSRLLKDMTEDFRNPVEKAK
                     RIKDVEHVGDGITHEIARKLNQTFITPIDREDIHDLASALDDILDVVEAIADRFVLYK
                     VTAPTESAIRLANVLYQAAVEVGATVDLLGKSHQAVTECSVRVNSLENEADRISRDAI
                     SLLFEKETDPIAVIKWKEIYENFEAGTDRCEDVANILERIALKHT"
     gene            complement(75247..76239)
                     /locus_tag="PK224_00310"
     CDS             complement(75247..76239)
                     /locus_tag="PK224_00310"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRB14211.1"
                     /translation="MKRSRIVGTGSYVPSRVVDNQEVAAPLGLDPDQIVALTGIRTRY
                     WVEQGQASSDLAVVAGQCACEAAGITPQSVDAILVSTTSPDSAFPSTACHVQRALGAN
                     PVMAFDLSASCSGFLYGVSMADVMIRSGQVRSCLVIAAEVKSRSLDARDRETAMLFGD
                     GAGAVLVVQEDSAQPDAPGILGVRLYADGSGHGLITIPAGGSRKPVGVDTVRASEHML
                     RMQGRAVFRSAVRRLEQAMIDVLKEFGLAAGDVTRVIAHQANARILEQLRRRLGLSQE
                     AVYSVIEQYGNTSSASLPIALDQAVRTQRLAAGDLVLLGAFGGGLTWATGLVRW"
     gene            76445..77365
                     /gene="bamD"
                     /locus_tag="PK224_00315"
     CDS             76445..77365
                     /gene="bamD"
                     /locus_tag="PK224_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRB14212.1"
                     /translation="MHRHSRGLCVGLTIVVGMLWFAAGCSSKPKTTADAKPVSGTDEQ
                     IFLGDTIEKNYDPNVIMKRGEAFFDKEEFAEAIVEYQHFLELHRAHQLAVYAQLRLAE
                     SHLRMAKSIDRDPEPIQKAIAAFEKLRKDFPGSKYEAQALQRIADCHDWLAQTHLFVG
                     QFYYRRASYLAAAHRFDQIMKDYPDKKVAPEALYYLALTYQELGADDWAMEKLQLLAE
                     KYPNSENAGDGRRLLAKLEKKSPPAPTVVAKTDTPAPQNQPSTTLLAASVPSSSFTPS
                     ATTPSIPNLKNSPALSLGQPFVSCRLGAWC"
     gene            complement(77544..78752)
                     /locus_tag="PK224_00320"
     CDS             complement(77544..78752)
                     /locus_tag="PK224_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HRB14213.1"
                     /translation="MADRQLATTYDVIIVGMGPAGAVAAAALCRGGLTVLGFDKDVHP
                     RYKVCGGGLSARIDLLLEPGFKSVVEQTVNGVQFVYRGQDPLLIESSQPIAYMVMRDR
                     FDHYLGQQAIEAGTEFRTGEGVVEVTQDSDGVEVVTQEGRYRAKMVIGADGANSLVAR
                     QLFPNRSLYRAPALESEVRLGNSRHYPSATTILVDVGAARQGYGWIFPKKNGVSVGVG
                     EFRRKSSGLRTTFDRFVSEARGLSGWTVPRPVGHPIPAYSDAEGGHGDGRGSRFVNGR
                     AALVGDAGHLVDPLFGEGIYYAVRSGQLAARSILGNVHDPRESLAAYEAALERDILPD
                     FRVTARIARVIYAFPRLGFRLLRRYQDVVQSYFEVLQGRTTAVQFLPDAKKRLKASVN
                     DLLLEALHLR"
     gene            complement(78872..79618)
                     /locus_tag="PK224_00325"
     CDS             complement(78872..79618)
                     /locus_tag="PK224_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRB14214.1"
                     /translation="MTDSVQSISLVAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDVSERNRFRKAIILNSLLFIAGFSTVFVAFGASASLLGQALITYQEHLRRFGGIVV
                     IIFGLYLLGILNLNFLKMEHRYQFRNRPAGFLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTESMLSGVVLLTFYSLGLALPLFLTALGVDRFLSYFKEVRAYLWGVSTVSGVLL
                     IVVGVMIYANSLTMITSFLERYGIGWYLGQ"
     gene            complement(79621..80142)
                     /locus_tag="PK224_00330"
     CDS             complement(79621..80142)
                     /locus_tag="PK224_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB14215.1"
                     /translation="MMLCGGFSADARGALPSSPAVPMLVVERIPQVDEEAPNFLLRDS
                     EGFNVTLDQFRGKVVLLNFWATWCGPCKIEMPAMERLYRSFSRKDFEILAVSTDAQGA
                     AVTRPFQREVGFTFPVLHDADFRIGLQYGARQLPMTFLIDRKGIIRNRIPGARDWSAP
                     AGKRLVESLLQGS"
     gene            complement(80190..80813)
                     /locus_tag="PK224_00335"
     CDS             complement(80190..80813)
                     /locus_tag="PK224_00335"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB14216.1"
                     /translation="MTDPINQSPANTAPRTGPSRTLVVIAAAAILGVMFLVVWLQSSK
                     YEPLVVGKEAPDFQLPDLNEKQLRLSDYRGKVVFLNFWATWCKPCREEMPSMEVLYKN
                     FEKDGLVILAVSIDRVTTKKEIPPFVKGLNLTFPVLVDSWGQTDKRYKLMGVPETYII
                     DQQGVLREKVIGPRDWTRLDNLKVLTQLLKPAEKAAHATPTQALPTS"
     gene            complement(80817..81731)
                     /gene="ccsB"
                     /locus_tag="PK224_00340"
     CDS             complement(80817..81731)
                     /gene="ccsB"
                     /locus_tag="PK224_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HRB14217.1"
                     /translation="MMRSLFLFDMTFWLYLAALGLYVAYLFAKRPAMQMVPAGHPIED
                     LEERDGAWATQLGQVATLVTVFGWVLNTLALTTRAFERMEHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLEFRYRIKAVGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGLMYLFKERAVNRGSQSRFYAAFPDLETIDELGYKAIML
                     GFPLLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGHKAA
                     IYAIFGFLMVIFCFWGVNFLLSGLHAYA"
     gene            complement(81809..83245)
                     /locus_tag="PK224_00345"
     CDS             complement(81809..83245)
                     /locus_tag="PK224_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRB14218.1"
                     /translation="MNDKPDAASDVSASAPRTSGLGWEEFSREVVDFFASIKLAMFLF
                     IVLAMTATIGTVIQQGERPETYVQEYGEEAYRWFLRFGFTDVYHTWWFTSLLGLLCVN
                     SLTCFHKRFPSVWRSMRQDKVSVSLAFIQGMKQQTTLSLNQSRDAVAERLVKLFAEKG
                     YRVLAKSDPGEVTVYATKGIMGRVGAHVAHLSATVIVLGGLLGSYYGFQEFGVCLEGQ
                     TYHIPRGNFDLRVDKFWIDYHENGSVKSYNSTLTVIDQGTPTTTKTITVNDPLVYKGI
                     WFYQSSYGDAWDQIEAARINIKEKGSDKIIATVDLEWNKEKAVDGLPLKMKMTDFVAD
                     FAFNSTEKKVFSKTAEHANPAIRLAVDERSSVQSTPWVFYHYPDLFEIKDSAYQFEFI
                     GYQPKKFTGLQIARNPGINMVWIGCTMLVVGMTLSSLIYHRRLWAKIIPGESGVTLHL
                     GGTTHKSQIDFQKEFRKLTEKINAQAQS"
     gene            83643..84791
                     /gene="metK"
                     /locus_tag="PK224_00350"
     CDS             83643..84791
                     /gene="metK"
                     /locus_tag="PK224_00350"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRB14219.1"
                     /translation="MRHNYLFTSESVTEGHPDKIADQISDGILDAIIAQDKFSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYTDASWGFDSNTCSVLTSIHQQSG
                     DIAMGVDSGGAGDQGLMFGYATNETTELMPMPIVLAHRLTKRLAEVRKKKILKWVRPD
                     GKSQVTVEYKDGKPCRVDTIVVSTQHSPDVTNKQIEKDLMEHVIRPSMPKGLYDPTSV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPSKVDRSASYMA
                     RYIAKNIVAAGLASKCEVQLAYAIGVADPVSVLVDTKDTEKVAPESLDKLVRKHFPLT
                     PRGIIDHLKLRRPIFRKTAAYGHFGRNEPEFTWEKTDKAKALRKDAGL"
     gene            84899..86155
                     /gene="ahcY"
                     /locus_tag="PK224_00355"
     CDS             84899..86155
                     /gene="ahcY"
                     /locus_tag="PK224_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRB14220.1"
                     /translation="MDYDVKDMGLADQGKLKIEWAEATMPVLRLIRKRFEREQPFKGI
                     RATACLHVTTETANLMKTLKAGGADVRLCASNPLSTQDDVAAALVRHEGIPTFAVKGE
                     DNKTYYRHIESAIAHKPHLSMDDGADVVSHLHSKRKDLLRNVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSTLIVAGYGWC
                     GRGIATRARGMGANVIVTEIDPLKGLEAVMDGFRVMPMDEAAPLGDFFVTVTGNLKVI
                     RGEHFAAMKDGAIVCNSGHFNVELDIPALEKLSKKKLAVRSGVDQYTLKNGRRVSLLG
                     EGRLVNLATAEGHPSSVMDMSFANQALGAEYIVKNYKKLEKKVYPVPADIDKEISRLK
                     LAGMGVSIDKLTKEQVKYLASWEMGT"
     gene            complement(86485..88119)
                     /locus_tag="PK224_00360"
     CDS             complement(86485..88119)
                     /locus_tag="PK224_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB14221.1"
                     /translation="MLVFLIHVRDPQFYALPAKTRAKNGRIRVMGFPPIGIMSLSSVL
                     KRSGHECVMFDQAHPETPNEFIIEEIKRRKPALVGLSFLSTTSYPYAKILARQIRAAD
                     STVKLAFGGVFASLNAGLVKLQCPEIDFVCRGDGEQLLLDLLDRLEDPQEVGGVTWAK
                     DGKVINNPNRTMERHLDQWPFPDRESLELDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCVFCDIPIFNEGKWRARSAQHVVDELKYLEANGYGSVYFVDDHFLLQPKRIDAICK
                     GIIDAKLTIQWGIEGRVDSVAQHLFPAMAQAHCRTVMFGIESGSQKILDRLQKEQTLA
                     EVETAVRNAKKAGIEIVHGFFTVGNPDETVEDMQKTFDFASKLPLDTFGFNRLCVYRG
                     TPLWQEYIKRGLVSDAKDWYKYFKCSEIDPTCLPGAVINSVRQEGLKKLFIYKLTRYP
                     IQTFKLLRRFLRFMPFRDVAYLIIKPFLGQKKGATKAEVLSRAVEHAEMKDAAAQLTQ
                     LSDELLHSVLEESKAERQRIQQEAEGSRELPMVNSR"
     gene            complement(88421..89293)
                     /gene="sucD"
                     /locus_tag="PK224_00365"
     CDS             complement(88421..89293)
                     /gene="sucD"
                     /locus_tag="PK224_00365"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRB14222.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKMVAGVTPGKA
                     GQEVEGIPVFNTVADAVKKTDCDTSLIFVPPPFCADAILEAADAGVKLIICITEGIPV
                     NDMVKVKRALHGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKRGVVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDSETQAVVMIGEIGGDAEEKA
                     AEYIKKHVKKPVISFIAGITAPPGRRMGHAGAIISGGKGTATEKMKALEAAGVRVVKN
                     PAEIGHAVKAALGR"
     gene            complement(89305..90483)
                     /gene="sucC"
                     /locus_tag="PK224_00370"
     CDS             complement(89305..90483)
                     /gene="sucC"
                     /locus_tag="PK224_00370"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRB14223.1"
                     /translation="MNVHEFQAKQLFAQFGVPVPRGKEITSPEAATAWASELNTPVFV
                     VKAQIHAGGRGKAGGVKITKEKGAVAGIAKELIGKTLITHQTGPKGRKVHRLLLEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAEKTPEKIIKEAVDPAVGFQGHNG
                     RNVAFALGLQTMEPAVINPFVQMLGNLYRLFMEKHAALVEINPLIITKEKTLVALDGK
                     VSFDDNGIFKHDDVQKMRDLNEEDPLEIEATANNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKDTVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKITVPLVVRLQGTNADEGRKLLAESGLKLEVANDLWEAAQKIVKLTGKAA
                     "
     gene            complement(90569..92170)
                     /locus_tag="PK224_00375"
     CDS             complement(90569..92170)
                     /locus_tag="PK224_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRB14224.1"
                     /translation="MDIHALQQIVHQTRDARRKQTIPKFSPADRDQLIHKYHPDYRTS
                     AYRPIRFGPNANDKTVVELAALLEGDSSIAENIDLTPHYTTDVLVVGGGGAGCAAALH
                     AHGTGAKVILATKLRLGDSNSVMAQGGMQISVAPEDSPVTHFLDTLKGGHMQNDHELL
                     KVMVEDGPSIAKWLIELGVLFDRQADGNLHVKKGGGSSKPRLLTCSDYTGLEIMRVLK
                     DEVLNQKIQLLEFCAAVELLSDDHGHCTGAIFKDLDNHRQVVVAAKSVILATGGIGRL
                     HIQGFPTSNHYGATGDGLCLSYRMGAKLDHIDTFQYHPSGAVYPEQLIGALVTEGIRS
                     EGGHLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRVGVWLDTPLLDAEHG
                     PGTVEKHFPAMMMQFERFGIDISKDPVLIYPTLHYQNGGVKIDANSETNVKNLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRSGLTAASRAGSMPQGKLTLQHLRRFRAEAKQHG
                     SSSGVISPMILPAYTRKEPERTANG"
     gene            complement(92240..92950)
                     /locus_tag="PK224_00380"
     CDS             complement(92240..92950)
                     /locus_tag="PK224_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRB14225.1"
                     /translation="MAQEDTNVIDQPEVLRHKMVTIEISGKKYEVPEGITVIKALWHT
                     GQEVVRGAGCLGGFCGACATYYRTKDDPKVRTCLACQMAVEDGMSFTIMPPFPARKAL
                     YDIQSLKDPKQDLFNLYPEAPLCRNCNACTEACPQKIDVREGVWKAVFGDFKSVSEMF
                     MDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFTEKPQGLDIRIKEIQDGRFNDEWN
                     KVLAMNEQELADHCATVK"
     gene            complement(93035..95266)
                     /locus_tag="PK224_00385"
     CDS             complement(93035..95266)
                     /locus_tag="PK224_00385"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRB14226.1"
                     /translation="MTTKADKIIYTKTDEAPMFATYSLLPIINAFSKAAGVSVELRDI
                     SLAGRILAVFPEYLTPEQRQPDALSELGELAKKPEANIIKLPNISASLPQLQAAIKEL
                     QSQGYKLPEYPENPKDDKEKDIKSRYDKVKGSAVNPVLREGNSDRRAPLSVKAHTRKH
                     PHKMGAWSSDSKSHVSHMKGGDFFSNEKSLTTAAATEARIEFVGQDGKVTVLKPKVAL
                     QAGEVVDATFMSVKALRTFLEEQIQDSAKQGVLFSLHMKATMMKVSDPKIFGHAVTVY
                     YKDVFAKHGETLKKLGVDPDNGIGDLYAKIKALPEDQRKAIEADIQAVYKQRPPMAMV
                     NSDKGITNLHVPSDIIIDASMPPVIRDSGKMWGPDGKLADTKCVIPDASYAPVYREVI
                     DFCKKHGALDPKTMGSVPNVGLMAQAAEEYGSHDKTFKAPGNGTIRVVDASGKALLEH
                     KVEEGDIWRMCQVKDAPIRDWVKLAVTRARATGAPAIFWLNKDRAHDAQLITKVNQYL
                     KEHDTNGLDIRIMTPADATRLSLERIKEGKDTISVTGNVLRDYLTDLFPILEIGTSAK
                     MLSIVPLLNGGGLFETGAGGSAPKHVQQFQEEGYLRWDSLGEFLALAASLEHLAKVAN
                     NPAAKMLADTLDQANAKFLESNKSPARKVGEIDNRGSHFYLALYWAQALAQQTQDKNL
                     AARFVKVAKEMADNEAKISGELIAAQGKPVDTGGYYHPDDAKSSKAMRPSTTLNAIID
                     AIA"
     gene            complement(95293..97539)
                     /locus_tag="PK224_00390"
     CDS             complement(95293..97539)
                     /locus_tag="PK224_00390"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRB14227.1"
                     /translation="MSMDLAKKLYAKMPEVFAKARKQFGRGLTLADKILVSHADNFDA
                     QTWERGKAMLALRPDRVAMQDATAQMAMLQFMQANKEKAAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENREVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGCLIIGTDSHT
                     PNGGGLGGLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGKLNGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDEKMVAYMTITD
                     RADLATFAQSHRDLLVADPEVYQSPEKYYDQIVEVDLSTLEPHVVGPHTPDLARPISK
                     LAAEAKAKGYPVELKAALIGSCTNSSYEDISRSAHVAEQGLKAGLKAKASFLVSPGSE
                     RIYHTMKRDGFLDTFEKLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGISETLSFLASPEVVTAYAITGDLGFDPVNQMLKGADGKEFKFQPPQGEELPAKGF
                     AKGEEGFVAPAESGEGLTVDIPPTSERLQLLQPFPRWDGKDFEKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANSAFTSEPGRGTNVLTGEANLTIAQIARDYKS
                     KGIGSVVVGDENYGEGSSREHAAMSPRFLNVKVVITKSFARIHETNLKKQGIVALTFA
                     DPKDYDKIEQQDRISVTGLNNLAPGKPVQVTIHKADGNALTIQTNHSITEQQIAWFKA
                     GSALNALN"
     gene            complement(97654..99474)
                     /locus_tag="PK224_00395"
     CDS             complement(97654..99474)
                     /locus_tag="PK224_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRB14228.1"
                     /translation="MSILANKDTRVVIQGGQAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDAGKSNEIPYGSGLIAIPVYKSIAEATKHHPTLNTSLVYIGADRAMKGGMEALDDS
                     HIKVVSMITEGVPEKDAKILGAHARKLGKVFNGPSSIGIISAGACRLGVIGGAFDNLV
                     LSKLYREGSFGVITKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRVKLIAVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKAGALVPPTFGALGPAIKETYQDLLKSGQIKEPVEPATLP
                     RLPKTIEEAMKADEVMVSPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGMIMIGPRF
                     GGAVTDAGRFFKYAVDNKMSVDEFLAYMKKNHGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLNIKTPCLDFALEVEKITSVKKDNLILNVDGTMAAVLVDIGFPVDSLNGFFILSR
                     TIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            complement(99517..100716)
                     /locus_tag="PK224_00400"
     CDS             complement(99517..100716)
                     /locus_tag="PK224_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HRB14229.1"
                     /translation="MAKVLEGPGMGLMKKWGINVPNYVVVTSVDELTRLGQANEWLKK
                     SKLVAKAHEALGSRFKLGLVKIDLDFKAAEAATKEMIGRQVGSITVTQVIVSEMIPHK
                     EEYYCAVKSTREGSEILVANCGGIEVESNWERVKRLCLEIGQAPSPESLEKLSKEAGF
                     GGPLAKKMAEFAGKMFACFDSEDAQYLEVNPVVLRAADEELVALDAVTLLDGDAKFRH
                     PDWNFAFAAEFGRAYSKQELEVMAVDSKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIRNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKSEGKLKSVKIWVRRGGPREKEGLDAMRALKEEGFDINVFDRNTPLTDIV
                     DKALQAK"
     gene            complement(100908..101771)
                     /gene="mutM"
                     /locus_tag="PK224_00405"
     CDS             complement(100908..101771)
                     /gene="mutM"
                     /locus_tag="PK224_00405"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRB14230.1"
                     /translation="MPELPEAEVAARQLRERVVGATVRDCWIGREDIVREGLPSLDQY
                     RQAKIAGVERKGKSVILHFLCGKEPRFLVAELGMTGLLLFRSTLTKHPQHTHFILHLD
                     GCCEPDIRYWNPRRFGRLSLLDRAGLDRYVARRFGHDPLTISHEKFLRVLRATRSRLK
                     SLLMHQQVIAGIGNIYANEILFRSRLHPNQPSNSIPGKSITRLHQVMGEVLREAIAMG
                     GSSVRDYFAPDGTEGQYKRRHLVYAKAGEPCPNACGAVICRSIGERSSFYCPTCQRNG
                     RRRATRPEKSR"
     gene            complement(101960..102382)
                     /locus_tag="PK224_00410"
     CDS             complement(101960..102382)
                     /locus_tag="PK224_00410"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HRB14231.1"
                     /translation="MINKRLLVGNADDARNPPPQVSAVLMVAEEQNVTVPSRVIYAKI
                     PLKEFGEPTAAALYEAVEWIAAHVADNRLMVCCRVGMGRSVSVVIAYLCCVEGMAYAD
                     AVKLVLTRRPGGMPLPRLQETIQDVCRRRQTQVNSSTS"
     gene            102509..102868
                     /locus_tag="PK224_00415"
     CDS             102509..102868
                     /locus_tag="PK224_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14232.1"
                     /translation="MAHTSLEVVEKRLASVQCAICKTNTFGVDRRTLQPDGECRAVCL
                     KCRYNFPVYTDMEFYLRTQPDVPYRLKEISCPSCQHRGVSLDFRITMSVREAIYFVTC
                     SACKTQFPERSSLEAFE"
     gene            102906..103100
                     /locus_tag="PK224_00420"
     CDS             102906..103100
                     /locus_tag="PK224_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14233.1"
                     /translation="MSTTPTPDPPKAPTPVTQAETKPRKEIPLISVPNDRDMIAAEMA
                     ELFDEDRVSHQHGSSEPEAD"
     gene            complement(103105..104190)
                     /locus_tag="PK224_00425"
     CDS             complement(103105..104190)
                     /locus_tag="PK224_00425"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HRB14234.1"
                     /translation="MFTRNGQVGSRVYFEDPQLCAGYLVYYLPVNLAKVQILLDELQP
                     VLPVAQDQDFRVLDLGGGPGTGVLGVLDWCLSKSARPPSTLRMIAVDRSPHVLRVSTD
                     IWRAYCEQQRDQSIPPLNTVECNLERSLPSAIRDGGGVNGYQLIIVQNLLSELFVGSM
                     DPVGQRTALVGELLNYLAPEGSLMLIEPATRPASRELHQVRDNLLAGQHCSVFAPCLH
                     DAPCPALLKPDDWCHEERKWKTPAWIAQVDREVGLLKDALKFSYVIFRKDGKTLVPRD
                     PDYHRVVSELRVMKGEKRVWLCDQAGRSEIGRQDKESSSSNAAFDDWHRGAIIRVSEI
                     VRKERKGRPAMVGRITSSGVVEIVRPA"
     gene            104376..104624
                     /locus_tag="PK224_00430"
     CDS             104376..104624
                     /locus_tag="PK224_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14235.1"
                     /translation="MTDPILQAYLEVEMAMERFTLVLHDHVDHLRKTEAPGSDKLHRM
                     ANGTKAMRDSASIYLSYAKYVAHGMPESPDLVEEDLQG"
     gene            complement(104718..106064)
                     /locus_tag="PK224_00435"
     CDS             complement(104718..106064)
                     /locus_tag="PK224_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB14236.1"
                     /translation="MSEALEPKQKTNPQGDPITSVSAPKAETKKDPHADAKRQKVVTP
                     EYMFLEAPRTKEFITGSEAAKEAIRRSNVDLAIAYPITPQSETMQLVGVLYGEGYVKE
                     YYRGEEEVGVMAAIAGGSRAGVRCYTATAGPGTLRGLEGIASWPGHRLPVVAMFTCRV
                     VNAPLAIQPDNIEVSYLLNCGMIVFHAENQQDMYDFTLAGFIISEKNDVTLPVGVCCD
                     GFFVTHARGYVRMQDRGMKLPPREPWRGAVPVLDAENPPARLSRDAPVQKSNFMAYNI
                     HAVWQQEVWAAVERSRKYINQYMGGLLTAENVDDAEAVIIASGSAAAQSREAVRICAE
                     KGIKIGLIKIRSLRPFPTQELRKLCGKAKLIVVPEFNYVGWLAKEVATAIYGFSNAKI
                     IGGPRVYGGQSMPVELIVDEVESGLTGKKSTNVAISQVMGASAADHEAMGHFMRSI"
     gene            complement(106103..106861)
                     /locus_tag="PK224_00440"
     CDS             complement(106103..106861)
                     /locus_tag="PK224_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HRB14237.1"
                     /translation="MTQDTRERIIVPGPAGFHPPSAAQLGVALPDPGEGLYYGLLEPN
                     EDIVIEEMARKMLTSPNATIFPGPLVLWAWNNHAVEKAKAVLEIAAQIPEVMIIPMPD
                     YRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCCTMA
                     ICAEQGHEDAMLTIRDSDTLKLKRTAQIFKKVREEMGIKLPDNGENVRFTGTQSKVHN
                     GKTHTNPMTFMPSAAGVGSAATFGHSAEQMKREG"
     gene            complement(107239..107952)
                     /locus_tag="PK224_00445"
     CDS             complement(107239..107952)
                     /locus_tag="PK224_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRB14238.1"
                     /translation="MAKRFNIRMAGVGGQGVVTGSHILSTAVINAGGESTIVPFYGSE
                     KRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHGKSYTMPFYFGLKEDGIAL
                     INNDGPMKLHRDQARELEERRARLYYFPATKLSLEVAGMDLCTNMALMGCIGAITGLT
                     TVEALEQSVKDRFLGKGFVVSGGTAALDSVVERKFKKKQELIDKNVAVIQAGWNYAVD
                     HGWAAPTVKRSEIRATVSA"
     gene            complement(107976..108875)
                     /locus_tag="PK224_00450"
     CDS             complement(107976..108875)
                     /locus_tag="PK224_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB14239.1"
                     /translation="MSLDYVKFSSGFEKFMPKEYRDMVEHGPFGKKVSVSQMGSFKEV
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTNRGAVLKMAPLGKKFEKMDMLQMAKVAGCAYVATVVPNNPRRVESVI
                     KKAVLIAREVGSTYIQAYTSCNIEYAIPTDKVMEDAKNVENDRYQFSEYITDEAKQFL
                     TERYGYKEFLPKPAAPAPGLPKA"
     gene            109341..109589
                     /locus_tag="PK224_00455"
     CDS             109341..109589
                     /locus_tag="PK224_00455"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRB14240.1"
                     /translation="MPIRVLVPGEEHGEQHAGGVHKRPPIPDGSLKAECPKFMSHGPC
                     GGVRKGGFCEVYPEMTCPWVTLYNKLNELGQLEWMKQV"
     gene            109613..110041
                     /gene="queF"
                     /locus_tag="PK224_00460"
     CDS             109613..110041
                     /gene="queF"
                     /locus_tag="PK224_00460"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRB14241.1"
                     /translation="MTAKGSKKRKPARATTKLGYNERHAKSGISAPLPDIETFPNQYK
                     GYEITIVIPEYTAICPKTNLPDFGTITLHYKPDKHCLELKALKTYIHAYRNVGIFYEN
                     AVNRILQDIVRACRPIKATVTGEFAARGGLRSVIEAKYPA"
     gene            110298..111686
                     /locus_tag="PK224_00465"
     CDS             110298..111686
                     /locus_tag="PK224_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14242.1"
                     /translation="MPVLRTQSMGATIVQAVVGLFVVISGTYDMSVQTVEAIPAFARK
                     YALNCTTCHTAPPQLNTFGERFLENGYQLPGTEDGGITGKKNLGDLSLDDFANYTGVR
                     LRGNVLRTHNFKQQNPPGAESGAVQNKSELGFPEVFSLFTAGTLSKNIGVFAELESNL
                     EEGTTGMERAFITFNNVGGENIANIRIGKLDPSAAFSFSTLRQQLELIGESTNSSTDA
                     VQRAGLFPLATAAKFYGLRDRSGAVISPYAPSLYNTVAETGIEVRGRPFGDWFMYQVG
                     MLNGSNEGFGDSNKGKDFYGAIRLDYARSANFSASLSGFAYLGNSNATVFDGTRTVDV
                     NWNRYGAAAHLRYKMLDFHGMYTLDRIKHVPTAALSNFDTTASGVTVALDAYVTNRTL
                     LSLRYDNMDAGGDLTQRTSQSFAGMQLKHYLRSNVAVYARHDLNLRRAEEGNAAAHNL
                     RHAVFVGIDISY"
     gene            111700..112473
                     /locus_tag="PK224_00470"
     CDS             111700..112473
                     /locus_tag="PK224_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB14243.1"
                     /translation="MHRHVLRYLAILIVTGFCATTAQALLPKDGRDTITEGHRIYDRA
                     CAWCHGSEGKGDGPSGWSIGRYAAPRPRDFTAESYKFRSTPSGELPTDQDLFRTITQG
                     IPGFMPSYRSLNEQERWQVIAYLKSLNPAFEQEQPTPIALPSPPPLPSGNGITNGRAL
                     YAKYECRTCHGDNGAGDGPESKAGHLRDAHNLPITATDLTDPSSFKNGATPHDLYRSI
                     MSGLDGTPMPAYADQFAGQEEATWDLVWYLQSLTGQPGR"
     gene            112549..113268
                     /locus_tag="PK224_00475"
     CDS             112549..113268
                     /locus_tag="PK224_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14244.1"
                     /translation="MKPILLTVTLCVILGPSALLRAESPTSTLVGRITYAGPIESGQV
                     VAVTRDSSFCGATTAIHTVTVNAKTRGLQGAVVSIDGDTVPMADSPRRPLVLTNTHCA
                     FSPRIVAGEVGQQLEIRNDDPVMHNTHISMGPRTFVNVAMIPASRPVEKPLKQPGIYL
                     VKCDAHKFMRATVLAFTHPFFSVTDDTGAFQIAQLTPGEHVVRVWHDTLGTFQQRVTI
                     PTRGEATITIEYPAHVDANKK"
     gene            113246..116173
                     /locus_tag="PK224_00480"
     CDS             113246..116173
                     /locus_tag="PK224_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB14245.1"
                     /translation="MSMPIKNRTDWFCRPWRIWHGNLRLRLSVGMAVLITIGMAGFAT
                     FRLIEIRQSIEQATQARALAVSRTFSMVGAAAVLDNLFRIQEALGRYAQDSEILSILI
                     IDPDNMIVAATNPQQIGQQLTDETLAQAHETNAETISHGQTMGGVPTLLVATPLLNEQ
                     DIATWVRIEFSLAAMQEELTREARRLFLLSVLFMGATISVAQLGIRRMSGLFRETAKK
                     LQETLHTLHCVSEQAPSGDETSDNGNSPASPSCKGELEQVVDLVETTTTLLTTQAQRL
                     QSFTDSLEQAVRERTIELHQAKEAAEAANRAKSQFLANMSHEIRTPMNGVLGMTELLL
                     TTTLTPKQRSLADTLHRSGTGLLDIINQILDFSKIEAGKLKLEMIEFGLRQTVEDAVD
                     LFAETAGRKHLELTCFVPPNIPDTVIGDPVRLRQILLNLVGNAMKFTHTGDVAVTLEL
                     ITHTTTNLTLKFRVRDTGVGIPEAAQARLFQAFSQADNSTTRKFGGTGLGLAIVKQLA
                     LLMGGDVGVDSVSGQGSTFWFTVELERAAQRTAASGATERPLDGFSIFIVDDNSTNRQ
                     ILETHLTAWGAAVLSATSGLEALALLDRQCTARRRIHLAVLDIHMPDMDGITLARTIR
                     EDARLSGMGLVALSSVDQVTDESQRYPSLFSAWLRKPVHQSLLKDCLTRLCHHVPATE
                     APAPTQPDDHAVPFAAHVLLAEDNPVNREVAFTMLELLGCTTTMVENGREAVEALTNR
                     PFDLVLMDCHMPEMDGLTATALIREQETQAAQPRHTTIVALTANALEGDRERCLAAGM
                     DDYLSKPFTLTTLKEVLHRRLSIKGPSRTTEPLLRSTPQAPPPQAAPPTGPAAPGDDH
                     VDLNAWQAIRARQRPGQPDLLHKALSLYLPHADAQLIQLQQAMAGADIQAVKTIAHMM
                     KSSTAQLGAPRLADLYAQIEATCRAGTVDNLPDLCGHLIPEHRAVCTLMRQELNRAGR
                     SAA"
     gene            116202..120497
                     /locus_tag="PK224_00485"
     CDS             116202..120497
                     /locus_tag="PK224_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB14246.1"
                     /translation="MRRFLQRLRQSVRRTSGQLATDSGDSQTEHREIPPSNTPLSLDE
                     GRALDAVLCSLDEGLCIVDAQWHILRLNPQAEILFAAPAHDLKGRPAYQLIAPGPEEF
                     RHECLVTDRSIPPLQSGLPYRTDNGLLLRQDGQLTPISLTITPMSHDGVVRGAVLTFR
                     DLTAQKEAESHQIENSALLRRLQTGMVRLASNQAIYRGQLTEAFHLITRVTTQSLRVA
                     RVSIWFYAPDHAVLRCADLYDQPGDQHSHGIEQPAALYPQYFSSLEAGEMIVANEAQA
                     DPRTVEFAAHHLLPLGITSMLNVPIRAEGILVGALHCEHIGTPRVWTTEEQQFAASVA
                     NTVSLAVEAADRHKAETETKRAEQFLDSVIENLPIMVYVKEAEQLRFTRWNRAAEELT
                     GFSRELILGRTGHHFFRKEEADRFTRHDREALRSGRLIEVASEEIHTQHRGVRLLRTK
                     KLPVMDEHGRPRFLLGIAEDITDRTAHESSLHESEEKYRELFESSRDAIMILSAPNWN
                     FTACNPATVQLFGARDAEHFTSLGPWDVSPEHQPDGEESMVKAPKMIGIALQEGSHNF
                     EWLHKRVGGPSFLASVQLTRITLKGPPGLQATVRDITEQRRSEAALQAYSTFQKAMLD
                     NAGHAIISCKPDGMIQVFNPAAEALLGYSADELVGKQTPGIFHDLDEVVARARQFSKE
                     LGSTIEPGFDVFVEKCRRNLPNEHEWAYVCKDGTRKTVLLNVTALREADGRITGYLGI
                     ASDITPLKLAAQELVSAKEAAEAANVAKSRFLANMSHEIRTPLNGVLGMTELLLSTSL
                     SAKQQSLLETVRRSGVALLEVINDILDFSKIEAGKLELEQVEFGLRQVVEEAVEIFAG
                     SAGNKKLELTCFIPADVPDSIVGDPVRLRQILLNLIGNAIKFTTQGEVAVAFALTKQH
                     ADTFTLACQVRDTGIGIPDAAQAQLFTAFTQADGSTTRRFGGTGLGLAIVKQLVQLMG
                     GEVGLVSAPGQGTTFSFTFTVTRGSAHAAYPPLAERHLAGRQILIVDDARTNLEILAT
                     HLRSWGATVFTAESADDALLQLEHFRHTQHRIDMAILDLRMPGRDGVDLARSIKQQTA
                     YQNLPLIALSSVERLSDDSDVTHRLFHSFLRKPVRQSLLKGRLTQVLGGSAGTVTPSP
                     QSSAAPRPRVEAHILLAEDNPVNQDVASSMLEMLGCRITIAPNGRAAVEAATTGRFDL
                     ILMDCQMPEMDGFAATAAIRRQEATASDRRHVPVIALTANAMEGDRARCLAAGMDDYL
                     TKPFTVAQLHAVLTQWLTPQPAKASETDQASSPHTTDADEPAATEEAPVSTTIDKTAW
                     DAILSLQRPGRPDILARVLATYLDDSRLLVEQIRSAVQSQDAVVLCQAAHRLKSSSAQ
                     LGVLATAARCKELETLGRLARIDEAAHLLSQLIEAHQFACTAITSELQQRSAR"
     gene            120564..121382
                     /locus_tag="PK224_00490"
     CDS             120564..121382
                     /locus_tag="PK224_00490"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HRB14247.1"
                     /translation="MPTYAIGDVQGCVASLRHLTQTIGFNPAQDRLWFVGDLVNRGPD
                     SLAVLRYIRGLGPAAVTVLGNHDLFLLAVATGLATLRRDDTLTQVLDAPDCDELTTWL
                     RQQPLLYREGSYVLVHAGLLPHWTIEEAQQLAMEAETALRGEQFSATLRALHPSGHLQ
                     WAPDLKGPVRLATIIKVLTRIRTCSDDGIMESTFSGPPKLTPQGFHPWFDLPNRRSAT
                     ATIVFGHWAAMGLHLTPNLCALDSGCVYGRQLTAVRLEDRKVFQVRCEETRGGK"
     gene            121369..121908
                     /locus_tag="PK224_00495"
     CDS             121369..121908
                     /locus_tag="PK224_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HRB14248.1"
                     /translation="MEVNEPPLTVDWSRYSSRALPTYRFLPGLTPHPRRDPLGHSFAE
                     PEPRPRPFAQHQWPDSEDYLYAVDLYNFGYWWESHEVLEGLWHACGRRTTAGNYFQAL
                     IQFAAAHLKRALGNEVAAHNLAHSGLLRLQNSPASYMGLDTVTFAHDITSWMGDHSRP
                     AVRIRLESFGNGPSVNEAP"
     gene            complement(122064..122852)
                     /gene="panB"
                     /locus_tag="PK224_00500"
     CDS             complement(122064..122852)
                     /gene="panB"
                     /locus_tag="PK224_00500"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRB14249.1"
                     /translation="MTVPDLQKCKRDRRKIAVVTAYDALFARMVEQAGIDVILVGDSL
                     GVVVQGKSNTLSVTMEEMLYHTKLVAGAAQRSLVIGDMPFLSYQVSQEEALRNAGRFI
                     QAGAHAVKLEGGAAVADRVSAIVKIGVPVMGHLGMTPQSVHQYGGYKVQGKGKDRAQQ
                     LLEDAQALEAAGAVGIVLEAIPAGLAKTVTEALTIPTIGIGAGPHCDGQVLVLYDLLG
                     LFDEFVPKFVKPYAHLKVDALQALRRYKDEVEQGTFPSDSESYH"
     gene            complement(122852..123742)
                     /locus_tag="PK224_00505"
     CDS             complement(122852..123742)
                     /locus_tag="PK224_00505"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRB14250.1"
                     /translation="MSREPRRLKDVLAQGQFAVTVEYNPPKGTNLTHVVESAKALVGR
                     VHGVNVTDNTAAIVRAGSLPVCRVLYELGHDPVMQLTCRDRNRIAMQSDLMGAHILGI
                     RNILCLTGDYPTVGDHKEAKPVYDLDSVQVMQLVTGLNNGKDYAGNKLDGSTAFTIGG
                     AVTPEADPLGPMLVKFEVKVRAGAEFFQTQAIYQPEQFKKFMEAVRPFKVKVLAGILL
                     LRSAKMAEFMNANIPGVCVPQDMIDEMRAAGDKRALDVGVEIAVRTIKAVRPYCDGVH
                     IMAIKSTERLPEILTKAELG"
     gene            complement(123915..124775)
                     /gene="folD"
                     /locus_tag="PK224_00510"
     CDS             complement(123915..124775)
                     /gene="folD"
                     /locus_tag="PK224_00510"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRB14251.1"
                     /translation="MAARIIDGKALAQQVRERLAVESAAVLAKTGVKPGLATILVGDD
                     PASHVYVRNKQKACELAGIYVDDHKLPASTTQAELLALIEKKNADPKIHGILVQLPLP
                     KHIESRVVLEAVSPNKDADGFHPYNFGRLVEGNPVFEACTPKGVIKMIESAGVSIEGK
                     RAVVVGRSNIVGKPLALMLLQRNATVTICHSKTKDLAAVCREADLLLVAIGKAKFVTA
                     DMVREGAVVIDVGTNKLPDGKLCGDVDFEPVSQKAGWISPVPGGVGPMTIAMLLDNTV
                     ESAKRMAGMK"
     gene            125032..126063
                     /locus_tag="PK224_00515"
     CDS             125032..126063
                     /locus_tag="PK224_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14252.1"
                     /translation="MNSRTIRHNRFTRLLRRRGFGLALALAIGVLFHQPWAWALQVSG
                     LESPHSFLADPATRSYYISNINGEADARDNNGFITRLSDDGRITAFKFIEGGRGDVTL
                     HAPKGMVVVDDVLYVTDLDTLRAFDKTTGKPLATLALPRPAAGGAAQSLTDVASDGQG
                     HLYLADQGGNAIYRVDLVPTLTLSLYVSGAHLAGPSGVAVHPKTGHVVVVSYDSGKIF
                     DITPEGTLTELASNGFFTARFQNLSGVDFDRWGSMYVSDGTKGKIWRMLPNGKFQVIA
                     EYLPGPSDVGIDRVNHLILVPYQDSNAAEVNGLESPTASSGDRAKRTLADYGFVEPKK
                     SDKEGSPRK"
     gene            126060..126752
                     /locus_tag="PK224_00520"
     CDS             126060..126752
                     /locus_tag="PK224_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14253.1"
                     /translation="MSRCVYCKQRKGKRSCPALTGLICSQCCGEHRLTRISCPPDCDY
                     LDTGSDYQQKRLGEQFAPVRRELYRQLSVAGGEKAAALFNLIEVVTFGYFHDRRDGQD
                     AEVFAAIQALRRTLSPLHVPSAPMPVFAERLKKEYDTFVKQQPQQVSDAGSAPEILDR
                     AVAILTEFSGQDFQSRRFLNGMIGYVKTFHPEIAEHLTKQHEAGRIVLPGQLTPPAQE
                     PTHVHGPDCHHH"
     gene            126946..128697
                     /locus_tag="PK224_00525"
     CDS             126946..128697
                     /locus_tag="PK224_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14254.1"
                     /translation="MGSFVMDLLASLPKGDALAEPAWNSRHRGILSILWLHVLSVPMF
                     GIYMGASPSLYLGGGGLLAFIAVAARLSVVRRRLQSAIATCGLMTASALLVHLSGGQI
                     ELHFHFFVMMSVIVLYQDWLPFLVGLQFIILDHGVVGTLMPDMVYGHAQGQTHPWTWA
                     LIHGGFILAECAALLYFWRVNELAQNEVRQSEARTRMIIETALDAVITSDATGTITGW
                     NTQAELMFDVPRTEAIGKYLTTYVSASYPSTEPGPITPYAGLVPGAILNRRSEMLGHT
                     CQGTSFPVEIAMSCLTVGGTQQFTIFVQDISERKQQEERLHRAKEAAEAASLAKSQFL
                     ANMSHEIRTPMNGVLGMTELLLTTPLNDKQRRYADTVHNSGTSLLHIINDILDFSKIE
                     AGRLVLEYIPFGLRQMLTETIGLYHEQARKKGLTLSVTIAPESPDMVHGDPHRLRQIL
                     TNLVGNALKFTETGNVAVFVRPDDGDQGRVRIEVTDTGIGIPLHAQEKIFDSFSQADG
                     SMTRKYGGTGLGLAIVKQLVGMMGGQLGLTSTPGQGTTFWFTISVDRSGSPETESQPH
                     RSDETTPTMSTRQQPDN"
     gene            128713..129861
                     /locus_tag="PK224_00530"
     CDS             128713..129861
                     /locus_tag="PK224_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRB14255.1"
                     /translation="MHVETCAIKTGSSFSPASLSTLDSEETLVLLFGARDLIDTPHRI
                     REVVDACPRSHVMGCSTAGEIHGCEIFDDSIAVAAVRFDHTPIRTAHAAVHSPNDSYA
                     AGRAIAAQLRQPSLRGVLVLSDGLNVNGSELVKGLNDTLGEAVVVTGGLAGDGTHFKR
                     TWVLKDRTPQSGYVTAVGFYGDHIRLGHGSKGGWDKFGPERQVTKSIGNVLYELDGRA
                     ALGLYKEYLGDRASGLPATGLLFPLAIRTSQAEGKVLVRTILAVDEATQSMTFAGDIP
                     EGVFAQLMRANFDRLIQGASEAATLTFDHHNGSHADSPTLSIAISCVGRRLVLGERTE
                     EEIEATLEILPKGSRQIGFYSYGEISPYKSGACDLHNQTMTLTTIAEH"
     gene            129867..133499
                     /locus_tag="PK224_00535"
     CDS             129867..133499
                     /locus_tag="PK224_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB14256.1"
                     /translation="MHPLLKRQLKRIGLDEATVPTSLDGWQQLLERVSQSYLESDQGR
                     ELLERSIALSSREMQELNEQLRRTSESQLAGERDKLQTVLRSIGDGLCVVDGHWNIVL
                     LNPEGERLWGLTEKEVVGRQLHDMISLSYGTKLTEPIFTQILLDDTAQGGSFRTDDGL
                     LTSTTGRSFPVSCVLAPIVQENRSAGAVLVFRDITERKQTEDIRRHRESLLRRQQTTL
                     LELTRNSIIQSGVLEPALQEITRATAMTLGVSRTSIWLLSEDHGSIRCKDLYESSKHS
                     HSSGIELKSANFPEYFRELLSERVIDAADARSDPRTREFTACYLEPLGIGAMLDIPIR
                     FNGKLVGVLCNEHIGPPRPWMLEEQQFGHAIASLVALALEAVERLHAEGALRKSEGRT
                     RLIIDTALSAVIGMDERGLIIGWNTQAEQIFGWTRQEALGRAMTDTIIPHAHREAHQR
                     GLERFLKTGDGPILNKRIEITALRRDGTEFPVELAITPLRIENAYTFTAFVVDISERK
                     EAEEALRTSEARLTMTVQGSHIGIWDWNLTTGVIYFSPQWKNQLGYDDITLPNNFDEW
                     RTRIHPDDQPFVQETIQTCLDGQRSQFELEHRLRHRDGSYRWILSRGSLIRDVYGVTA
                     RMVGIHIDTTDRKQAEQELRAAKEAAEAASKAKSEFLANMSHEIRTPMNGVLGTTELL
                     LNSALTDKQRHLASTVHRSGRTLLAIINDILDFSKIEAGKLDLECVDFDLQPVLEESL
                     ELFGEAARRKQLRLSQHIDDAVPRYLKGDPVRFRQILMNLLSNAMKFTETGGVSLSAE
                     YVEGTTTQALLRFAVTDTGIGIPPAAKSRIFDAFSQADGSTTRRYGGTGLGLSIAKQL
                     VGLMGGTITVESTPGSGSTFAFTTRLDLQPLGSGLSTKLAPYMQWAEPLVSSGQTSDT
                     PCPHASEAGAVAPSPKQSGRILLAEDSPVNREVAVGMLEQLGYEVEVAENGRQALAAA
                     ERDRFDIVLMDCQMPEMDGLTATGEIRRRETASGRRRLPIIALTAHAMQGDREQCLTA
                     GMDDYLTKPFTQMELHGIVRKWLSHHEPASQAVQPNAAAPGTQSVPPRATTEPAADTL
                     SASPGMDLKALEGIRALQRPNRPNVLASVLRKYLDNSRESVDALRDAIRANDPMALEA
                     MAHRLKSSSAQLGAIAVAASCKELEVMGNRKQLVDADRIFAQLESDYRAACTVFRNEI
                     AKENQT"
     gene            133496..134743
                     /locus_tag="PK224_00540"
     CDS             133496..134743
                     /locus_tag="PK224_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14257.1"
                     /translation="MNPLIPGRAPYALISDDDIIIRMFAREALEQAGWVVEEAQNGIE
                     ACEIFQKCPPDVVLLDVMMPGMDGFATCAALRRLPGGEHTPILIMTGLDDFESITKAY
                     DAGATDFIVKPLNAMLLTHRIRYMVRAAQVLQELLESQATLTQARDAAIEGARLKSEF
                     LATMSHEIRTPMNGVLGMTDWLLDTPLTAEQLDCAQTIRSSGDALMVIINDILDFSKI
                     ESGKLSLELLDFDLPTFLDRVLALFGERAQRKGLLLTSRIAEDVPSGLCGDPARLQQI
                     LSNLLANAIKFSEHGSVSVLVELDQRPAEQGLEFPATRYGELSAQQERVTHIRFSVSD
                     NGIGIEPSAFSKLFQPFVQADGSTTRKYGGTGLGLAICKQLVELMGGHIGAESEPGSG
                     SVFRFTVPLQQQLPNSLSTRQAA"
     gene            135076..135969
                     /locus_tag="PK224_00545"
     CDS             135076..135969
                     /locus_tag="PK224_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HRB14258.1"
                     /translation="MHCLTFDIEEHFQVSRFDSPIRRRHWGSFESRVTPNTCKVLDLL
                     ARYQTRATFFVLGWVAERHPGLIKQIAECGHEIASHGYGHELVTAQTPELFRADVRKA
                     KQILEDLTGSPVQGYRAPGFTITRETLWALPILAEEGYTYDSSIVPIRHDHCGLPGSD
                     PWHHLRQTSSGPIWEIPPSTVNLGGVRLPIAGGSYFRLLPFPALCGLLRRTEQKGRPL
                     VMYFHPWELDPLQPHMEGPLLSQFLHYLNLDKMEFRISTLMNTFRFGPIAEQIDLSPI
                     MPLDPTIFPALPPAEIAQDVA"
     gene            136128..136703
                     /locus_tag="PK224_00550"
     CDS             136128..136703
                     /locus_tag="PK224_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14259.1"
                     /translation="MHHGSRNRRQQSPNTTGLAWALACIILLPAAAAGQSLDDTVQFI
                     SDMANTHGFVRSLSCRNPKAGKPTKITEIYSVIPTGTKLGTIELNHGHDEVNTGFTHF
                     DLHDIDTIEYQGQDTKENNLVLYGVRITCKSSGPCIMKTSFCTGAVTELEAQFPEDTL
                     LFRSASHAERMTKALAHFLGLVRAQRQNQPF"
     gene            complement(136769..137419)
                     /locus_tag="PK224_00555"
     CDS             complement(136769..137419)
                     /locus_tag="PK224_00555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRB14260.1"
                     /translation="MAQQRWSNRLRIAGMMSLFMTVGIAGIGLAADAAPAGSESVSAS
                     NARAVIKTKFGDIEIKFLPDVAPKHVENFIKLAKSGFYNGTIFHRAIPGFMIQGGDPN
                     TKDSLKKGAYGQGGPGHNVKAEFSDLPHKRGVVSMARAQDPDSAGSQFFVVVEESRFL
                     DRKYTIFGEVVKGIGVADKIVALPRVMCQTGAPSPADKGPCDNPIDRVEMTVTIIE"
     gene            complement(137457..138161)
                     /gene="rnc"
                     /locus_tag="PK224_00560"
     CDS             complement(137457..138161)
                     /gene="rnc"
                     /locus_tag="PK224_00560"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRB14261.1"
                     /translation="MTPATSIEAVQRLLGYRFHQPRLLEEALTHKSYSNERRSKDRTQ
                     NERLEFLGDAVLSLVMSEYLAAEFPGSNEGGLSKLKAHLVSEASLAKAARRMKLGRLL
                     RLGKGEELSKGREKHSLLADALEALIAAIYLDGGLEASRKFTLQVLEEELLATRAEQA
                     RPGMEDYKTQLQEVCQKRYESLPQYETVRESGPDHEKVFEVELTIQGVMRGIGRGRSK
                     KEAEQMAAKEALTQLA"
     gene            complement(138189..139439)
                     /gene="fabF"
                     /locus_tag="PK224_00565"
     CDS             complement(138189..139439)
                     /gene="fabF"
                     /locus_tag="PK224_00565"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRB14262.1"
                     /translation="MHDRPARRVVVTGLGLVTPLGTGVEKTWKALCAGESGIGRITRF
                     DPTGYDAQIAGEVKDFDPAQFIEKKEIKKMDTFIHYAVGASQLAVDDAKLKVAPEEAT
                     RVGVYIGSGIGGLGSIEHYHDVLREKGPGRVSPFFIPMTIINLASGQVAIRVGAKGPN
                     SCAVTACATGNHCIGDAYRLIQRDDADVMIAGGAEAAITPLGVAGFASAKALSFRNDD
                     PTKASRPFDKDRDGFVLGEGAGVVVLEELEHARARGARIYAEVIGYAMNSDAYHITAP
                     PEEGEGAVRCMEMALKDAGVAKTDIGYINAHGTSTMADAIETKAIKHVFGEQAYRIPV
                     SSTKSMTGHLLGAAGGIEAVFSILALHHGILPPTINLDHPDPACDLDYVPNTARSVQT
                     QVVLSNSFGFGGVNACLLFRRCAQ"
     gene            complement(139453..139686)
                     /gene="acpP"
                     /locus_tag="PK224_00570"
     CDS             complement(139453..139686)
                     /gene="acpP"
                     /locus_tag="PK224_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRB14263.1"
                     /translation="MATVDERVKKIIAEQLGVEEEEVTLEAHFVEDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKILTVGKALDYIKEKA"
     gene            complement(139786..140529)
                     /gene="fabG"
                     /locus_tag="PK224_00575"
     CDS             complement(139786..140529)
                     /gene="fabG"
                     /locus_tag="PK224_00575"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRB14264.1"
                     /translation="MSLQGKVAIVTGGAQGIGRAIAEALAEDGADIAVADLDPARSQE
                     AVAAIQKLGRRALSVKVNVADWNDAKAMADQVMQEWGKIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGTFHCTKAVLLPMTKQRYGRIVNIASIVGAMGNVGQANYAASKAA
                     VIGFTKTVAREYASRAVTVNAVAPGFIDTAMTQGLSPEVKEALQKQIPLGRLGLPSDI
                     AAAVRFLVSDAASYITGQVLHVNGGMLMV"
     gene            complement(140559..141512)
                     /gene="fabD"
                     /locus_tag="PK224_00580"
     CDS             complement(140559..141512)
                     /gene="fabD"
                     /locus_tag="PK224_00580"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRB14265.1"
                     /translation="MMASQIGFLFPGQGSQSVGMGRGFYEASPAVKAVYDEASSILGY
                     DVAQLCFEGPAERLNLTENTQPALLVSSAAALKALEPAGLKPVAVAGHSLGEYSAVYA
                     AGGVSFRDAVALVQKRGRYMSEAVPPGTGLVAALLGLSAAVVKAVCQEAASVGVVAAA
                     NFNSPGQVVIAGEKAAVERAIEIAKTKGCKKAIPLPVSVPVHTPLMQKAADRLAGEFG
                     GVVWRDLTVPLVNNAEATALQRSEDIRASLVRQLPSSVRWEESVQTMAKLGVTTFVEI
                     GPGTVLTGLVKRILPGAVTANVNDPKSLEATLRMLGVNSQA"
     gene            complement(141619..142596)
                     /locus_tag="PK224_00585"
     CDS             complement(141619..142596)
                     /locus_tag="PK224_00585"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRB14266.1"
                     /translation="MIRARITGTGSYAPERVMTNADLEKLVATSDEWIRERTGIRERR
                     IAAEGQACSDLGLIAAERALKAAGISADELDMILLATCTGDMPLPSTACLLQHRLGAT
                     RAAACDVSAACCGFVYALGVADAYIRTGMRHILVVGSEVMSTITDWTDRNTCILFGDG
                     AGAAVVSASEGERGVLSTHLHSDGSLSDLIVVPGGGTRIPPSEAMVSERSQYIKMKGN
                     ETFKVAVRTLEEVARETLAAHHLSVDELDVYIPHQANLRIIKAVADRLNLPLEKVVLN
                     MDRYGNTSAASIPIALDEAVRDGRVKEGQLVMFGAFGAGLTWASALVRW"
     gene            complement(142593..143624)
                     /gene="plsX"
                     /locus_tag="PK224_00590"
     CDS             complement(142593..143624)
                     /gene="plsX"
                     /locus_tag="PK224_00590"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRB14267.1"
                     /translation="MKIAVDAMGGDHGPAPVIEGAMQAAQELGVGIILVGKQDELTAA
                     CQKLNCTDTRITIHHAPQVVEMHESPATVARKKRDSSIWVATELVKSGEADAVVSPGN
                     TGASMVASFFVLGLIKGVERPAIATMLPTLTGSAVMLDVGANVDCTAQHLEQFALMGN
                     EFAKHVYAKQNPRIGLLSIGEEDSKGNEVTKEAFKLLKASPLNFIGNIEGREVYSGNA
                     DVVVCDGFIGNVALKISEGVADVIKKLLMKEISGSWLGRLAYPLIAKPLLNLKRKIDY
                     AEFGGAPLLGVNGTTMICHGRSSAKAIKNAIRRAKGLAETRLDELIQRDIEESLRRHQ
                     DERPEGKQA"
     gene            complement(143776..143961)
                     /gene="rpmF"
                     /locus_tag="PK224_00595"
     CDS             complement(143776..143961)
                     /gene="rpmF"
                     /locus_tag="PK224_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRB14268.1"
                     /translation="MANPKHKHSRSRRDMRRTAKTRLVPPGFSLCPQCHELKLPHYTC
                     MNCGTYKGKAVIVVEES"
     gene            complement(144000..144605)
                     /locus_tag="PK224_00600"
     CDS             complement(144000..144605)
                     /locus_tag="PK224_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRB14269.1"
                     /translation="MTVLMDTLKPAIVDIAPEGLSLSCTATGPELGLDEPEEKCEGPL
                     TIQLELVQHDGPITVTGTLEGTAIRQCVRCLTEYSDPLFVNLYAEYLPQAGLATKPAP
                     AEQGRRGPRRGAQPVEPVEEAEEADEVYLYQGEHLDLVPMVREQVILAAPMQPLCRED
                     CLGLCPQCGQNLNERRCGCPPEQGPSPFRVLRGRLSKGGNA"
     gene            complement(144690..144947)
                     /gene="rpsR"
                     /locus_tag="PK224_00605"
     CDS             complement(144690..144947)
                     /gene="rpsR"
                     /locus_tag="PK224_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRB14270.1"
                     /translation="MERGDRGNGGGGGGRFFQRRKPCRFCVDKAPIDFKDVGLLRNFL
                     TERGRIVPRRISGNCLQHQRVLTVAVKRARQIALVSFAEER"
     gene            complement(144963..145355)
                     /locus_tag="PK224_00610"
     CDS             complement(144963..145355)
                     /locus_tag="PK224_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRB14271.1"
                     /translation="MAGFNKVILVGNLTRNPELRYTPSGTPVASFGLATSRRFKQGDE
                     LKEEVCFIDIVVFGKQAEHCGQYLSKGNGVIIDGRLQQRRWETEDGQKRSKHEVVAQG
                     VTFLPKRGEAGGESGGLHEEPSYEDEQL"
     gene            complement(145339..145692)
                     /gene="rpsF"
                     /locus_tag="PK224_00615"
     CDS             complement(145339..145692)
                     /gene="rpsF"
                     /locus_tag="PK224_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRB14272.1"
                     /translation="MELYESLFIIRPTLSDEETTALIEKMKGTVTKNGASLERAENWG
                     RKKLAYEIKRERKGTYVYFYFKGPGAVIAELERAYRLEDSIIKFLTVKLEQEPAPPRG
                     APVVAPQGATVGGVQ"
     gene            complement(145945..146310)
                     /locus_tag="PK224_00620"
     CDS             complement(145945..146310)
                     /locus_tag="PK224_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14273.1"
                     /translation="MTRFVPGFIVLCVILLLAGCAQSVHQAERLAQSDFLVDPGYAGS
                     QSYSYWPGPGYGPGYGIGYGPGYWGHPLGYYGGPYGPLGFGWYGGYGGFGAARPPSGT
                     PGPRPSLPPHAPPQFKKKL"
     gene            complement(146375..146677)
                     /locus_tag="PK224_00625"
     CDS             complement(146375..146677)
                     /locus_tag="PK224_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14274.1"
                     /translation="MTRSIGSLFVASALLLLTACAQSFHQGERLGSVSAHMNPGYPES
                     PSYWNGPGFWWNPFGYYGSGYGWYGGGYGIGSQQSNAPAPRPTPPANAPPQFQKKY"
     gene            complement(146689..147330)
                     /locus_tag="PK224_00630"
     CDS             complement(146689..147330)
                     /locus_tag="PK224_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB14275.1"
                     /translation="MVWTRFVCSRLSAGLLGCVLLLCSPSPIRAEWYVAAQLGANFAD
                     PLRNVRGSGTLAGLDAPNFNLKTSPAFGGKLGVFPGHGLFGIELDVSHSTPHIKNLDD
                     VPGTHLSLTNIGAHVVLRYPGVTWQPYIGGGPALLVARLGRSSTTESDTQVSVGGNVL
                     VGIRAFVTPKVALFTEYKYTDSTFRFGGAFGPVGGFDATYRAHQLFVGLSYHF"
     gene            complement(147493..147705)
                     /locus_tag="PK224_00635"
     CDS             complement(147493..147705)
                     /locus_tag="PK224_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14276.1"
                     /translation="MRKPGEPIYLWIHLLALLLVIIATVALPRAAEFVVGPLSFGTRA
                     LAGVGIAVAGGIALYLLYNSSARNEP"
     gene            complement(147795..149000)
                     /locus_tag="PK224_00640"
     CDS             complement(147795..149000)
                     /locus_tag="PK224_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14277.1"
                     /translation="MTTSSHQSLSLAQFDLSPDRGFLPATDPLAQLPDEPILNQIGAE
                     LPKFLTARQFRSFVQDHQEVMGPVADDWGLDVFRCAMRTLSFAGHAFVWEDPDHPASL
                     IPASLALPWCAVAQQLGRPPVLSYASYALHNWRRLDPQKPIELGNVVLLQNFLGGLDE
                     EWFVLIHIDIEAKAGPGLVGLVQAHNAARDNMPDDVLQGLHALAAAQEAMRETLLRMP
                     ERCDPYIYYSRVRPYIHGWKNSPSLPQGVVYEGVADYAQRPQQFRGETGSQSSIVPAL
                     DAGLGIPHADDPLTQYLVEMRDYMPPRHRTFLAALEARTDEQHRPLLYGYARDRKATH
                     PDLWKAFCTCVDLLAQFRDIHIGYAERYIFQQHQSHASNPTAVGTGGTPFMPYLSKHL
                     EETKRFTLD"
     gene            complement(149128..149718)
                     /gene="pth"
                     /locus_tag="PK224_00645"
     CDS             complement(149128..149718)
                     /gene="pth"
                     /locus_tag="PK224_00645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRB14278.1"
                     /translation="MRLLVGLGNPGADYADTRHNIGCWVIERAAARWSIRLTRKGAAQ
                     RGSGRAGSKLVELASTLDWMNLSGPPLKGLLRELGLTPEALVVVHDDLDLEPGRVRIR
                     QDGGSGGHNGIKSVIEALGTPQFVRLKIGVGRPAPRQDTADYVLEQVSPEERAIYAPC
                     LERAVDALELLLNRDVATAMNQFNVREKAEGGERPS"
     gene            complement(149724..150491)
                     /locus_tag="PK224_00650"
     CDS             complement(149724..150491)
                     /locus_tag="PK224_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRB14279.1"
                     /translation="MKFDLTVESREGGGKGPARQLRRAGRVPAVLYGQGECLLLSVKP
                     DELVKILRSQAGSTALISLAINGAKSKAKRTALLREFQVDPITGSVLHADFFEVAMDK
                     PIRVKVPVHLTGGQPLGLKEGGVLHHVLRQLHIECLPSVLPDFIEVDASQLQINQGIH
                     LKDIPKTEGLRFLDDLEHMVVSVAAPMTDAKLEALLTAGAPGAEGAKEPEVAAKGKAV
                     AEGAAGAEAGKAGDAKAGAAAPKAGAAPAKKEAEKKK"
     gene            complement(150529..151470)
                     /locus_tag="PK224_00655"
     CDS             complement(150529..151470)
                     /locus_tag="PK224_00655"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRB14280.1"
                     /translation="MNRELKLFSGSANPALARAICSYLDLPLGAATVSSFSDGEIRIR
                     VEENVRGADVFVTQSCCSPVNDSIMEMLIMIDALKRSSANRITAVIPYFGYARQDRKD
                     QPRVPISAKLVADIITTAGADRVLTMDLHASQIQGFFNIPVDNLYALPVLMDYITKRK
                     VSDLVVVSPDAGGVERARAFAKRLQANLAIIDKRREGPNQAQVMNIIGDVQGKSALLL
                     DDMIDTAGTIVQGAQACLDKGAREVWAGCSHGVLSGPALERLQQSGLTEVLVTDSIPL
                     RGKEQKCPKLKVLSVAPLFGEAIRRIHEDESVSSLFV"
     gene            complement(151769..152668)
                     /gene="ispE"
                     /locus_tag="PK224_00660"
     CDS             complement(151769..152668)
                     /gene="ispE"
                     /locus_tag="PK224_00660"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRB14281.1"
                     /translation="MNSTSLRVLTPAKVNLILRVLERRPDGFHAIWSLMHTVGLQDEL
                     TLTVQPGTTSRIALRCDHVALAADQSNLVYRAAQLVLARIDRPVDLSISLTKRIPLGA
                     GLGGGSSDAAATILGLTRLLGLNWPIEQMAELGQQLGSDVPFFFMGPAACVTGRGEQV
                     RSMQMTGTRWIVLVNPGFPVETKWAYQQLSATRVGVRPLSPALQQLGSRAAVDWSEII
                     PLVENDFEAPVFSQHPVLAQIKSQLLSQGAEVALLSGSGATMFGVFPGQADAERAASV
                     FALDPKMKAYAVPAAGTPVTSMV"
     gene            complement(152689..154068)
                     /locus_tag="PK224_00665"
     CDS             complement(152689..154068)
                     /locus_tag="PK224_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB14282.1"
                     /translation="MEKILVVDDEQGLRDVLSIMLKRAGYAVTVASDGDEAIAQVQKE
                     IFDLVITDLKMPRVGGLEVLKAVKAASPDTVVLMITAFASADSAVEAMKHGAYDYLTK
                     PFQVDEVQLIIRNAIERRRLSTENMLLKRELASQSSFSRIIGQSEAMQKVYDVIKKVA
                     DSKSNVLIGGESGTGKELVARAIHFNSARASMPFVTVNCSAVPETLLESELFGHMKGS
                     FTGAISNKAGLFEVANGGTIFLDEIGDTTPAIQVKLLRVIQEREFRRVGGTQDVKVDV
                     RIVAATNRDLEKAVAEGAFREDLYYRLDVIPIKLPPLRMRTGDIPLLSQHFLEKFAKE
                     SGKPVPTMSQEAMRVLLAHEWRGNVRELENVVERVVAFTTGSSVTDADIRGWLHKPVS
                     NQQALPSELPEDGLDLEGLINTIEKDLLLKALERSQWVKKRAARLLRLNTRSFRYRLE
                     KYEIKGGRD"
     gene            complement(154053..155747)
                     /locus_tag="PK224_00670"
     CDS             complement(154053..155747)
                     /locus_tag="PK224_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14283.1"
                     /translation="MPELKTRLHWLMGLRVVVVTLMLGLSLAFQSTRGELVPTFSALI
                     ILTYATTIVYALLLRRLSAPAALTAFTWVQVGLDFVLETVLVARTGGIESPFAVLYVI
                     TVTVASLVPHRRVGLVTGAGCTLLFGLVTAVQYFGLLHASGWLPASRLEGAETLQTFG
                     VYGLAFLVVGFLSGVLADQLRHADQSLREKEQGLNRLQVFHESIVRSISSGVFTTDEE
                     GRITSFNPAAHEVTGYAFADVQGRLWRDVFNWHPGTPSEEAALTSLSPLRFEVECSHA
                     DGSRLVLGMTVSPLHEQGTQRGLVCVFKDLTQLRYLEQEMRRREWLANLGEMSAGMAH
                     EIRNPLGALAGAMQMLRQDVGSDDTSQRLMDIAIREARRLDNIITEFLQYARPPALNL
                     AEHDLNKVLADTLDLIQHEARSRSGITIVSRMAPNQLMAQVDQDQLKQVFWNLATNAF
                     DAMSAGGTLTIATGVRRVEVGGRRSDVIEIAFQDNGEGIPKQNFDKIFLPFFTTKKEG
                     SGLGLAQVHRIVELHEGWIKVESEVGHGARFVVCLPQSAETGVRLRHEGREPWKRF"
     gene            complement(155954..157171)
                     /locus_tag="PK224_00675"
     CDS             complement(155954..157171)
                     /locus_tag="PK224_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRB14284.1"
                     /translation="MSTFAYVGRNRQGAVKKGELTAKTRDEAVDQLRKQQVVVTSLEE
                     KSGMGGKFKFSLGSGLTDKDLVVFTRQFGTMINAGLPLIQCLDILSTQSENKVLRETV
                     GDVKNSVEAGSTFSDALKRHPKVFDDLYVNMIHAGEVGGLLDTILTRLAKHIEKAMKL
                     KGQIKSAMVYPTAIVGVAVIIISVLMVWVIPVFAQMFLEMSGGKVGLPGPTQIVINVS
                     NFFQSYWYAMGGAMVAAAIAIKRYYATVNGRVVIDRLLLKVPIVGDLIRKASVAKFTR
                     TLGTLITSGVPLLEGLSICAKTSGNKVIEEALMNARVSISGGKTISEPLAKCNVFPKM
                     VTHMIAVGESTGALDAMLGKIADFYEDEVDQAVETLTSLLEPIMMVVLGTIIGFIVIA
                     MYLPIFTMAQAIQ"
     gene            complement(157332..157868)
                     /locus_tag="PK224_00680"
     CDS             complement(157332..157868)
                     /locus_tag="PK224_00680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRB14285.1"
                     /translation="MNVPNSLTMLRILLIPVYVGLLNYEQFDYALATLFIAGLTDALD
                     GIIARVADQRTRLGEVLDPLADKLMLTTGFITLSVMHLVPLWLTILVVSRDLMLMLGA
                     AVAHFTHTQVDISPTVLGKGTTLVQLATLVAIIFFASRRLDLATLDPLLYLMGGVTLM
                     SGLHYLSRGYCRITSSQV"
     gene            158045..158365
                     /locus_tag="PK224_00685"
     CDS             158045..158365
                     /locus_tag="PK224_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14286.1"
                     /translation="MSGLPLLFKKEGLIERHQVEGIDPSDRYFNRAVLVSRVAAGYTG
                     KVTYEAYAVEGSAHSTTGAAVKAVVEKLMGVGFTRLRTRLNFKGNRYLAEKETWTDYP
                     DLPA"
     gene            complement(158362..159489)
                     /locus_tag="PK224_00690"
     CDS             complement(158362..159489)
                     /locus_tag="PK224_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRB14287.1"
                     /translation="MATTNPLQAALQAAVDDGAFPGAVLAVRLRGTLIYEGAVGRLSP
                     QIPGEGVTIHTCYDLASLTKVLATTTALLLLMQRGKLTLDDRIDRILIALQGSAAGAA
                     SIHQLLTHSSGLPGWRPYYERLASLEAGQSGFPGRTAAREAVLGYIAQEALVYERGSR
                     SLYSDLGFMLLGWAVERLAGESLDQFCNNQIYRPLEAQPLAYVPRESQAMPAASLESH
                     AIAPTEDDPWRGRMLCGEVHDENAFALGGVAGHAGLFGTARAVLAVAKAWMDGRRGKT
                     GLLQPDLVTLFTTRQQGIPNASWALGWDTPSAPSSSGTRFTPESFGHLGYTGTSLWID
                     PVKELEVVLLSNRVHPTRKNERIKTFRPLIHDLICREFLGD"
     gene            complement(159604..160116)
                     /locus_tag="PK224_00695"
     CDS             complement(159604..160116)
                     /locus_tag="PK224_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRB14288.1"
                     /translation="MKITPIDIQQMVFQVKFRGYDRDEVNRFLEELALTVENLNRENS
                     ALREKLTATEQQVTDLRRTETTLSNTLVSAQTLAEDVKRSAQRESDLIVKEAELKASE
                     IIRQARVSLSDMQRGLADLQKQRLMMVERFRSTLRSFERMLEVEESDAYQSDAASVEG
                     KLTGESSTAR"
     gene            complement(160138..160434)
                     /locus_tag="PK224_00700"
     CDS             complement(160138..160434)
                     /locus_tag="PK224_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRB14289.1"
                     /translation="MFVMSNVLQGTATVLDTVLWLYMWVIIARALISWVNPDPWNPIV
                     QFLERATEPVLTPIRRLVGWRMGMDLSPMIAILILVFLQYAVVQSLRDIAVRMH"
     gene            complement(160434..161240)
                     /gene="proC"
                     /locus_tag="PK224_00705"
     CDS             complement(160434..161240)
                     /gene="proC"
                     /locus_tag="PK224_00705"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRB14290.1"
                     /translation="MLTKPIAFLGGGQMAEALIGGLLAAGACEPASICATDPVAARRD
                     ALKSRFGIRVGDENAKAVREADLVVLAVKPQAMPAVLSDAGQACAGKFIVSIAAGVTT
                     AWIRERIVTPRGIVRAMPNTPALVREGVTALTCQPDLQSDDLAAVRTLFEAVGSVVSV
                     EERLMDAVTGLSGSGPAYVFVAIEALADGGVRMGLPRATAELLAAQTVLGAARMVLER
                     GEHPAKLKDQVASPGGTTIAGLYQLEAGGLRSCLMAAVEAATKRSQELGR"
     gene            complement(161245..161952)
                     /locus_tag="PK224_00710"
     CDS             complement(161245..161952)
                     /locus_tag="PK224_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRB14291.1"
                     /translation="MDAALGTIADRVRTVLDEMQRAAARAGRPPESVRLIAASKTVSV
                     ERLRQAVDAGIRHLGENRLQEALPKIDTLGREGVVWHFIGTLQRRKVKSVVGRFETIH
                     SVDSLALAEEIDRQAKTAGLRQRVLLEVNLAGEASKGGFEPTTLGAALESLNGLEHLD
                     IRGLMAIPPPTPTAEDARPYFRQLRTLAQTLTARGHRNINMQELSMGMSHDYPVAIEE
                     GATYVRVGTAIFGARGE"
     gene            complement(162053..162796)
                     /gene="pgeF"
                     /locus_tag="PK224_00715"
     CDS             complement(162053..162796)
                     /gene="pgeF"
                     /locus_tag="PK224_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRB14292.1"
                     /translation="MVSEVITAPSFATPADGVEHFFGTRLSSVSVTPGRAAVPGALHR
                     ERGASVILSVKQVHGTDALIVDRPVEEGDAFEGGWDALVTDQPGLMVTVRTADCVPVL
                     LHDPERRVVAAIHAGWRGAVAGIVPKTVALMVSRFGATVDRLQMAIGPSAGPCCYEVD
                     EPVLARLREVFPEWRSVVKPVSSQKAHLDLRAFVRRQALADGLAAERVATVDACTICQ
                     PERFYSYRREGVVKATMVSGIALLPRRPG"
     gene            complement(162837..164039)
                     /gene="ftsZ"
                     /locus_tag="PK224_00720"
     CDS             complement(162837..164039)
                     /gene="ftsZ"
                     /locus_tag="PK224_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRB14293.1"
                     /translation="MFSFQEEPQSPVRIKVIGVGGAGCNAVNTMITGGLCRVDFVAAN
                     TDVQALERSQASYKIQIGPERTRGLGAGAKPEVGRDAALESKDEIRESLVGADMVFVT
                     AGMGGGTGTGAAPIVASIARELGILTVAVVTKPFQYEGHRRMSHAEEGIRDLGRHVDT
                     LLIIPNQRLLGIVDKATPLLDAFKVADDVLRQAIQGIADVITTIGLVNVDFADVRTIM
                     AHTGRAVMGMGIGRGANRAQEAAQKAICSPLLEEGSVEGARGVLLNITGGPNMSLHEV
                     EEAASIVQHAADAEANIIVGQVINPEIGDDLIVTVIATGFEREEQPAARPAVTAERPA
                     ARTPNGRPAQQVLTGVHAAGSDRPHKDLDRPTFLRRMGETREAVERIAVVGDDEWDVP
                     TFLRKQAD"
     gene            complement(164094..165338)
                     /gene="ftsA"
                     /locus_tag="PK224_00725"
     CDS             complement(164094..165338)
                     /gene="ftsA"
                     /locus_tag="PK224_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRB14294.1"
                     /translation="MSRVSKRDHILVGLDIGTTKICAIVAEVPEEGPLNIIGVGSCPS
                     RGLRKGVVVNIESTVESIKKAVEEAELMAAVQINSVYTGIAGSHISGENLKGVVALKK
                     QEVTRDDISRAVESARTLAVIPHERRILHVLPREFMVDDQEGVREPLGMSGNRLEVNV
                     HVITGAVTSAQNIIKSVNRAGLDVVDIILQPLASSEAVLSAEERELGVAMVDLGGGTT
                     DLAIFLDGSIRHTAVLPIGGQNLTKDLAIGLLTSQTDAEKIKVQHGIARTELVHGHQM
                     VEVPSVGDRPPRQFTRRDIAEILEPRVEEMFDLVKREIVRAGYEGMLGAGVVITGGTS
                     LLEGMPDAAERGLNLPARRGMPTGIGGLRDIVSNPMHATGVGLLLHARQHADNLEAAG
                     IRHGKGLGKVFDRMRSWMFEFF"
     gene            complement(165335..166204)
                     /locus_tag="PK224_00730"
     CDS             complement(165335..166204)
                     /locus_tag="PK224_00730"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRB14295.1"
                     /translation="MSLRWRKVRPVKVNPRANARSESALGRHRAGETGGYWSRLGRGL
                     LITLRVVATVTVLVGGCSGLFVLAREVGPLTREWFLVRSVSVSGLHHVTRKEVIGRLA
                     LKPDTALYSINPSWLADRIKTHPWIKDATVVLKPLHEIHIDIVEREPAVVVRTLAENL
                     LADSDGFLLAHLGSADDPTLPMLSGVDGKRLVQGKPDDRRPVQVGAALARMVGQTTGG
                     RPDINVGNLNNLVVEVQGVTFQFSESSMNQQWYRFLKMRPALRDVAFDGEGARANEID
                     LRFADRVIVRGRG"
     gene            complement(166207..167205)
                     /locus_tag="PK224_00735"
     CDS             complement(166207..167205)
                     /locus_tag="PK224_00735"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRB14296.1"
                     /translation="MKGVDVTERKPLTRSKIGVLMGGQSSEREVSLKTGEAVYRSLVR
                     SGYDAVAIDVGPDLSKTLQEQAVEVGFLALHGPGGEDGAIQGFLETLGIPYTGSGVRA
                     SAIGMHKVVTKTELAAQGIPVPRGTVVLRGTAPSLNRVLSTCKLKLPVVVKPASQGST
                     IGVTIVRKPSQWKEALRTAHRYDEEAMVEAFIQGHEVTVSLLGGPDGVVTGLPAVEIV
                     APDGFYDFSAKYEKGRTQYLCPAPLPAAVTRQIKQLAIRTYQALGCSGAARVDFRITP
                     KGKPFVLEINTVPGMTETSLLPMAAGKAGLSYDALTERILQSALGRAGVGSFRAVR"
     gene            complement(167215..168147)
                     /gene="murB"
                     /locus_tag="PK224_00740"
     CDS             complement(167215..168147)
                     /gene="murB"
                     /locus_tag="PK224_00740"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRB14297.1"
                     /translation="MSRAAGKDHAARTRKDWARILEGVRGAITHEASLQAYTSFHIGG
                     PAEVLVEPADVEDLCRVVAQARAEHIPVFVVGGTNVLVRDGGIRGIVVSLRQFKAIRQ
                     EPDHVLYAEGGVGMPTLIGYAIRRSLAGLEWGAGIPGTVAGCVVMNAGTRLGEMKESV
                     KAVRMVDPRGRVVDIPAADIPFSYRRAHLPRGIVAGVWVQLRPGDHDRIEKTVKEYLQ
                     YRKNTQPLTLPSAGCVFKNPPQDSAGRLVDAAGLKGARIGDAQVSEKHANFMVNVGHA
                     RAADVLALIRKVRAAVKKVSGVKLELELKVVGEA"
     gene            complement(168169..169665)
                     /gene="murC"
                     /locus_tag="PK224_00745"
     CDS             complement(168169..169665)
                     /gene="murC"
                     /locus_tag="PK224_00745"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRB14298.1"
                     /translation="MRPATALAPYEQRERETQQQDRSSGQRSFRGQELRRTAMFRKTQ
                     HIHLVGIGGSGMSGIAEVLLTLGYKVSGSDLSQSETTRRLEELGGRIAIGHHESNIGD
                     AQVVVISSAVAATNPEVVAAKARQIPVIPRAEMLAELMRLKFGVAIAGAHGKTTTTSM
                     VANVLAQGGLDPTMVIGGKVNALGSHARLGRGDLLIAEADESDGSFLRLAPTIAAVTN
                     IDREHLDHYGTMERLNDSFLEFVNKVPFYGLAVLCSDDERLRNLLPRVVKRYQTYGLH
                     EHPGHVPDFRATDISLRQWGSEFRVFFRGRNLGPFRLSIPGIHNVSNSLVAIAIGMEL
                     DIPVDLIRKGLAAFSGVERRFHLRGEKAGIMVVDDYGHHPTEVRATIAAAKQGWDRRV
                     VVLFQPHRYSRSRDLMQEFAHAFDQADALFMTEIYAAGEQPIPGVSGAKLVEAVQAAG
                     HPSATFVERKDTLAEQVLPTLKSGDLVITLGAGDIWKTGLAILERLPA"
     gene            complement(169634..170755)
                     /gene="murG"
                     /locus_tag="PK224_00750"
     CDS             complement(169634..170755)
                     /gene="murG"
                     /locus_tag="PK224_00750"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRB14299.1"
                     /translation="MNVVIAAGGTGGHLYPAIAVAREFLKRDPSTRILFVGTTRGIER
                     KVLAHEGFPLQCIDANPLMGKSLLEILKALITLPKSLWQSLGILKKQSADLVFGVGGY
                     TSPAMLLAAFLRRIPGVILEPNAYPGMANKAVAPFVQRVFLAFESTVTFFDRCKTSVV
                     GTPVRQAFVESAAPDAEQAEARTARHLLIFGGSQGAKAINSAVIEALPLLRSMKDQLT
                     ITHQTGEADHARVVAAYEQAGMPAQVVPFLYDMPTVLRGADLVVARAGAMTIAELTVC
                     GKAAILIPLPTAIYNHQLRNAEVMAKAGGAVLLPQAELTGPGLAQTVIQILKEPHRLQ
                     TMSEQSWNMRRSDAAETIVRECYDVIRRRHEASDSARAL"
     gene            complement(170752..171960)
                     /gene="ftsW"
                     /locus_tag="PK224_00755"
     CDS             complement(170752..171960)
                     /gene="ftsW"
                     /locus_tag="PK224_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRB14300.1"
                     /translation="MAQHALGTLMLPWSTSTQRATKRVPVDPALLAVTLILALVGVVM
                     VFSASAVVAGNRFHDPWYFLKRQLAWLGAGLLVMHLISKIDYTIWKKLAIPLLFGTTV
                     LLVLVLVPSFGSVAKGARRWLHLGPLNIQPAELAKYAVAIYIAAYLTKKQDQITNFSR
                     GLLPPLIVLGLLSGLVLLEPDLGTVVVMGLVVVTMLFLAGARIKHLGLLALCALPTVA
                     ALILGSPYRRRRVIEYLYGAKDPTGSGYQIHQSFLAFGSGGPFGVGLGEGKQKLFFLP
                     EAHTDFVLALVGEELGLMGTVTIVLLFGLFVIKGFQIARRARNPFGRHLAMGITLLVG
                     MQALVNAGVVTGLLPTKGLTLPFVSYGGSSLMANLFGVGILLSISRDRQGGQDSSGPR
                     VVRKRGVVTE"
     gene            complement(171996..173378)
                     /gene="murD"
                     /locus_tag="PK224_00760"
     CDS             complement(171996..173378)
                     /gene="murD"
                     /locus_tag="PK224_00760"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRB14301.1"
                     /translation="MKDLQVTVVGLARSGVGAARLLNHLGARVTLADRKEAQELTAIL
                     SQVERSSIAVKVGAQYESALEGADLVVISPGVPTQLDALNRVRARGGRVIGELELASR
                     FLTAPVVAVTGTNGKSTTVTLIGKFLQESGKRAFVGGNLGIAASEAALASVQAKAGTV
                     APYEYVVLEVSSFQLETMEQFHPWIASILNVTLDHMDRYASVEDYVAAKANIFANQTA
                     GDYSLFNLDDARVAALRGRTKGTVLGFSRGGATVSGVAGATVLDGDLIVTTVRGRREE
                     ICRRSDMRLIGLHNVENVMAAVTYALLCGCSIEAIRAVLRSFPGLEHALEVVRERRGV
                     RFVNDSKGTNVDAVLKALEGIDQPIWLIAGGRDKGGDFSRLEGAIRERVKGLILIGEA
                     AGRIQDAMGDFDRCRPAATLRDAVELAAREAQPGEVVLLSPACASFDMFADYQDRGRQ
                     FKALVQGLPA"
     gene            complement(173395..>174241)
                     /gene="mraY"
                     /locus_tag="PK224_00765"
     CDS             complement(173395..>174241)
                     /gene="mraY"
                     /locus_tag="PK224_00765"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRB14302.1"
                     /translation="LIIFAVVLSTLLWADMTNRYVWLVVVATVGFGAIGFADDYLKFI
                     KRQSKGLSAAQKFTCQILVALTIGVFLYTLPSYTTKLSVPFFKFFTPDLGWFYIVFVI
                     LVIVGSSNAVNLTDGLDGLAIGPVMIASLAYTIVAYVTGNRVMAEYLLIPYIEGAGEI
                     AIFTGAILGSSLGFLWFNTYPASVFMGDVGSLPLGAALGTVAVISKHELLLLLVGGVF
                     VIEALSVILQVGSYKLRGKRIFNMAPIHHHFEMKGWDEPKVVVRLWIIAILLALLSLS
                     TLKLR"
BASE COUNT        37860 a        55367 c        46646 g        34368 t
ORIGIN      
        1 gaggggtcaa tttcacatca gaataaaagg gtcaacttcg gctgatgatt gacacactcc
       61 aatggaagca gattaactgg acagagggga aactctttct gaaggcccag gataccaaaa
      121 cgaatactcc acgggtgctc tatttgaccg gggacctcta tcgggtgtta ttggcctgga
      181 agacccgttg cgacaagaaa tggcccagct gtcaatggat ctgccatcga ggcggcattc
      241 gcctgcagag cttcaaacgc tcctggtggg aggcctgtga agccgtcgga ttagggagca
      301 gggtgaagga tgagaagaag ggacggcccg tgtgggatgg gaagagacct cacgatttcc
      361 gtcgtacagc ggtgcgaaac atgattcggg ccggcgtgcc ggaaaagatc gccatggcca
      421 tttctggcca caaaaccaga agtgtctttg accggtacaa catcgtgaac gaacgggacc
      481 tcgaacaggc agcacgatca ctcacggcct acttcgaaaa acagacggtt acacttgcgg
      541 ttacactggc agaattgagg ggtgagagca gcggtgtcgc aaaccgccaa caggttgaaa
      601 cgtcggggat atttctggag ccggcgagtg gaatcgaacc accgacctgc ggtttacgaa
      661 accgctgctc tgccaactga gctacgccgg cttgttgacc tgaacatcat cttcggatcg
      721 gtcaaggagc gcaatcttac tcaggggatg gaagcactgt caatgattga aacgcctccc
      781 gttacagagc agatagaaaa atgggatgat cccgtaattg ttcgaacatg atctgcgcct
      841 tgacctcctc gcccgctgga gagaggtgac aattgtcctg aaaccattca tcttcaaacc
      901 gatcttgggg cggaataaca aagggcaccc gataccgggt cgcgatttcc cgcatgacgt
      961 catagttctt ttccagcccc agaatcagag cctcttcgaa ccctttgaag gctcctgcat
     1021 tcttgctgag attctgcctt ctcgcttgca agaaaccgaa caacaccacg gctgagccgg
     1081 cctgttgact cgaagacacg agcatgtcga gattccgtcg gaacccttcg ggcgcctgca
     1141 ttcgtacccg ttcaagcgcc gttttcggat ccacttccca aaacggctcc gttaccacga
     1201 ctgcacctat cggctccagc cacctggagt agacgtattt tgctgtgctg ctgtgctgca
     1261 gcagaaattt ctctcctggg cgtggaatct cacccgcccc ataccgtctg aagtgggtat
     1321 attcgggagt gtaattcccg aaaaaaagcg gcagtacgtc gtttccgccg ccatgataga
     1381 tcacgatatc gggctggaga aaacgatgtc gaaacacata gccggcgagc gcctcggcgc
     1441 tggttccgta gttcagtccc gcattgacta cctgaaccgt cttcttcgaa atcgtcttga
     1501 gtttagcctc gaggcgggcc acccacgtat ccgccgggtt cttcacgaac ggccacatga
     1561 aggtcgtcga tccccccagc accagaattc gaatggtgtc cggtggtttt gcgacagtga
     1621 attctctatt ccgatagcca agcgaattgg tctgttgata cccgtccgtc gtcaaattgg
     1681 gcagatgcgt gtagagcaag tagggatggg acgcgagtga cgcgctctct ccgttgacat
     1741 cagagcgaac ggtcggctgt tgccccaggg acagatcgat gatccgcttt ccattcgaga
     1801 agggcgtgcg ctcatagagg taccctgtga cccattccag aacgataatc gaacaactaa
     1861 cgctggctag ccacagacat gccaggaaga ggattcgacg ggcccgtggc catcgtgttc
     1921 gtccgatcag catcaccacc gccttccgga aggtagagtg ccaccctcaa cggatcttac
     1981 tcagaggaat cccggttaac accggttgcc gaccacccat cggcagacag agtgtgggct
     2041 ggccaccccc cgtcgctcct cgcgtaacgg catcgacctg atcatgcacg tattcgcaga
     2101 gttccccgga caacacagtc ccgcttttgt cgagatcggc cgctccgcgc aaccccttca
     2161 gcaggaaata tgtgaacaac ccatgttgtc ccggctgata ggcttgcgcc tcctgtaggc
     2221 cggagtttcc gatcatcagc atgacccgat ctggctcccc gcttgaatcc tgttgggccc
     2281 atcgcggagg gaccacccgt cccggatcga aaccggacga cggctccagg gagagatcca
     2341 gcatcaacac ggcttgcttc acggaggctt tggtcagagc ccgctgaagc cgggcgaggg
     2401 aaaacatccg ggatgcaccg gctcgcgtcc catcgtaagg caacaacgag acgatcccgg
     2461 tctcttgatc caccacagcc cggccggaca gataaatata caccgtcgcc tgcggactgc
     2521 cctgcttggg caaccactgc tcaaacactt cgatcaagtc atctctaagc gcgttggcgt
     2581 cggacagcac cttcaccttg tgcggaggaa cgcccagaac ggtcttgaag tacccgccca
     2641 tgacatcagc atcgtgtgcg gcgaacttca ccgtcggcac ggccggatcc cggaacgcgc
     2701 cgataccgat cgcgatcccg acactgttcg gttgcgccga ctttccattc ctgctcggga
     2761 gttggtccac atctaccgac aatacctcta cagcggcacc ggcatcagga tgtatcgcga
     2821 caaaaaactt ctttgctgac gggagcagga cgttggatga cgctcgcagg gcacacatca
     2881 actcggcctg ctcggcgtct gcgaccgccc ccaccttgcc gtcaacggcc acgcggcgaa
     2941 cttcacccgg ctgcagcgtc cccaacgaga ccggcgactt gaggcctccg acgatcgcgg
     3001 catgaccgct caattcaacc tcgactccct ccgcaactac agccccctcg ttcttgactt
     3061 caaactccac actgaacgac tcccgttgtt ccagaacatg attctggtta tcgtctcgga
     3121 tgatggtccg gaacgagagt tgagtcatcc cggatcggat agccgaatcc ggggctgcgg
     3181 cctgcggcgt ggagccggca gcggattgca agggagctac cggaaccacc ggtgtggccg
     3241 ccaatggagc cactgccggt gtcgcaggcg gcacgggagc agactgcatg gttcccttgt
     3301 cgccgctctt tctgaactgt gcctgctcgc gaattttggt ggcagtgcca agctgttgcg
     3361 ccataccttc cacaagcttg gcggccgcct cttccgccac tttgtcgagg ccgctgatat
     3421 tgcaggtgtc ggcccgcgtc tcgacttctc cggtcgcagc gctctgcaat ttcttgctca
     3481 caaggaccgt gccgtggtga tcggtatacg aaaactccat ccccagcgcc accgtcgcag
     3541 gataggattt attggcctta cgaggaatga acagatccac ctggcggact cctaacgcgg
     3601 tatccaccac accatccacg gcaccagtcg atgggcctgg ttcaaggtgc acgcgttcga
     3661 atacctgccc catgcgcttt ttcaattgcg tctctaaccc gtcacgaatg ggcagggtga
     3721 gggcctggcc acaggcatcg cgatagtcta gcgatgccgc agcgatactg tgatccatcc
     3781 gaagctggac ggtgagtggg agattgtaat gagtggccag gctgctacgc tgaccgaaga
     3841 attggcatcc gctcgacacc cataccacga acagcagaaa ggcgagagga cacctcgaca
     3901 gccgccgcac acttggtcct attcctgggc acatagatga ctgcacaata gtttcgttat
     3961 accgaatctc acaaagacat aacaaccgac gcgatgccgc gttgacagtc ctcctgtgct
     4021 cggctatggt ccagcgcgtg aacgctgcga gtccttcaac tcaccgctca acacccgcat
     4081 ggtcctggag cgcggtggcc gacctgatcc gactgcggaa tcaatccggc acctggctct
     4141 tactgttgcc cagcctgtgg tcgttagtcc tggcaaatca gggccgtcca ccgatgtggc
     4201 tgttgggcgt ctttacgttg ggcgccttcg tgatgcggag tctcggcgtg gtctttaacg
     4261 acctggccga ccgcaatttc gacaaacacg tcgcacgcac acgcactcga ccgctcgccg
     4321 caggccgcct tgcgcccaga caagcgctgc tcgtcgcact tggcctggca gtgatcgcgg
     4381 ccgctctggt gatcacactc aatcccttca cgatgctact gagtcctatc gctctctttc
     4441 tggcagcgat ctatcccttc agtaagcgat ggattcagat tccgcaaggg gtgctgggca
     4501 ttgccttcgg ttggggagcc gtgatggcat gggcggcctc gcgcggctcg atcgaagcgc
     4561 cggcgtggtg gctctttgcc gcgacgacct gctgggccat cgcgtacgac acaatttatg
     4621 cgctgcagga ctgcgaggat gatcgccgca tcggagtgaa gtcgtctgca cttctcttcg
     4681 gtgaggctgt cccggtcgcc gtcggagttt tccttgtggg gatggtactg tgcctggtag
     4741 tggccggaca gttgtccggc ctcgggatgg gatattatct aatattggcc ctgccgaccg
     4801 gagtcttcct ctggcaggta cgacgattac aagcgcccgt cactccgcac atcgctttcg
     4861 ccatgttcaa acagcacgtc tattccggcg tggcgatcct cgtcggtatc tggatcggca
     4921 cctttaatca gactccctag ccggttattc gcccaccttc ccgagtggct ggtctggctt
     4981 cggagcgcgg agggttttca gatagagagc caccgccttt gcatccgcat cgttcaatcc
     5041 caagctgggc atacgcgtat caggcttcat ggcttgcggg ttgcgaagcc atcggtagac
     5101 ccaggtggga ttcagacggt atccggcacg atccagcgca ggcccgacct ttccaccctc
     5161 gtcgccgatc ttgtggcatc cattacaccc atacttggtc acataaatct gcttgccgac
     5221 ttccaccagt ttcgcggcgg catccggctt catattcaga tcgggacgcg caatgtttac
     5281 gcccttgccg ggcctggtcg taaaattcgc cagtgcggcc gtggcctggt cgagttcctt
     5341 tttggcctgc cccattgcct gcatttcttt ggcataagcg ttatcgtcga cttcaacacc
     5401 cttcttgtcg gcttcgtcgc tggctttctt ctccgcttcc tgagccaccc gttccgcttc
     5461 cttaaatttc tgatcggctg cctgctgggc cgcttgaagg tctttctttc gaccttccac
     5521 attgaactct aatttctttc cgtgcaacga gcgcacatat ccgacgatgc cccagatctc
     5581 ttcctctgac agcgtgtact tgaaggtcgg catggtcggg acggcaaatt catcatcacc
     5641 gatcttttcc ccgcctggcc ctgtgtcctt catatcgcgg gaaatggtgt taaagatttc
     5701 ctcatcctta aaggtgccca tttcgtcttt gttggaaagg tctttgggct tcgggtccgt
     5761 catggacgac caattgaacc cttcgttctg tctgccattt tcaccgtggc aatgcatgca
     5821 atagtgagca tagagcttct tgcccttcgc ggattgttcg ttctgaaaca tcgaacaacc
     5881 cccggctcca atgaggcccg agagtcccac caccaccgcg accgctccaa atacgcgtat
     5941 tcttcgccac gccctcatgc tcacgaccct ttctttaaac gtctcacgag gaccaactca
     6001 aagcccagcg ctaaggccac cgcgagtaat gcatacatgt acggcgaata gtccggcggc
     6061 gcatcggccc ggaaatacca ccaggacgaa atagcttttt cagagccctt ttccttcggc
     6121 tggccggtct ccatcttctt gccgtcccag acggcgaagg cgatgctcgc gaacgtccct
     6181 ggcttgaatt gggtatcttc ggccacatgc tccgtgctta acggacgcga gaagaccact
     6241 ttccagacgc caccctggta gacacctttg gctttgacat cctggtgggc atgaggcttc
     6301 agcgttccga acccttttgc cccaaggtct gcgcccttgc cgtccttgtt cttccaatac
     6361 cagatattga ccgggccgcc ctccacctga gccatgggct ggccgtgggc gaaatgggcc
     6421 ttggtatcgc cgaccatgaa ctccaaggct gctgcgtcct ccgggtcttc agacggatcc
     6481 gaatactcca gaagaatcgc caccgtggtc ccgtcatgaa gtccgcgaac cttgacgctc
     6541 ttggccgtga cgtcggtaat acgaaccggc caatgcacct gaggagacat tgggaattcc
     6601 gataccgccg ccgtggccca ggccgctgcc gtcggatcat ctgccggaag ggatcctttc
     6661 acgagcgcgg cgcgcaccga cacgctctgc tggctatagg ccggctgcac ctcggcggcc
     6721 acgcacagcc ccagaaggcc gacggctcca acccaccatc tctttcctac gctcctcact
     6781 cgtgccatac gctcccctta attgctcagc aatctctgat cggaggaatt actcggactc
     6841 atcccacgtt cttggtttct gtccggcccc gtcatattcg cccataataa ctttccagat
     6901 gtcctcatcg gacatttcca gttcccatcg cggcatagcc gtcttccatg gcatcccttc
     6961 gacaggaagg cctacgcctc ccttcttgat ccgccagaag agatagcttt cctgcagcat
     7021 ggcgatcgtc gtcggatcct taaagttagc cggcggcggc gtgaatcccg ccgcagcgaa
     7081 accctttcca tcgaagttgc cgtggcatgg cgagcaacgc gaggcgtaga gccccttgcc
     7141 ataattgata ttgtccagcg tcttcggaac gggattggaa agaccggtat actcaccggg
     7201 aggagcggga tgaatcgtgc gattttcacc cggtggtgca tcactggagg ccgtgctccc
     7261 gtaggcctgc catccgacga gcaagggaaa taaggccaac acgagatacc gtgctccact
     7321 cagggccccg ccaccttctc cagtgagaaa gcgcaggatc ggtccaaaaa atgcgtcttt
     7381 tgatgccccg ctgagcgtag caaaaatgac gatcccggcc gtcgtcagca tcaggtacag
     7441 gaaaatcaag cttgagggca gcggcgcgga atgcggcaga ttcgggacaa taaactttaa
     7501 gaacagataa atacagaccg tcagaataat cggtcgaacg agtacgcctc ccataaccac
     7561 cctcctctac ttcaccgacg actatcgagc ctgtgccacc gcgagcgttc cggcatccgc
     7621 cgccttgacg ggagccgggg tcgcccgcgg caaatccaat tcagtcggac tcacgctgat
     7681 cggctcaccg ctcatctgtt gcaggaatgc aatgaccgac agaatttcgt tcttggacag
     7741 gccgattgga ttcttgttga tatacggcat ccgggccgga tactccttcg gaactccctc
     7801 gtgtcggtag tccagataaa gatacgcttg cggctcggtc agactctcaa aaataaactc
     7861 gcgggacagc ttggccccga ttcccttcag atccggacag cgtgccgatt cgctcgggcc
     7921 gatagaatgg cagagcgcac actggccctt gctgaagaag atcttctgcc cgatcgtcgc
     7981 catgtccgtc ggcgtcttga tatcggcgat gttgatgctt tcttcagcag gcggcaatga
     8041 ggccatctgg ggaacggcca gagcgaccaa tgaaaacagt cccagcacga tcgccacgaa
     8101 accaatgacc cgaatgaatt gccccttgat gaacaggagg atcactgttc cgagtccgac
     8161 gacacagaga ccgattaact gcagtttgac tacttcactc ataagtcctc aacaaaggat
     8221 aatgatcacg acccacccct ggtaaccagg ggaagatcat cttcccgatc agaccggacg
     8281 tttttcctca gaagcatcga cctcaggccc atccagcgcc ggcgcccccc cggccatcgc
     8341 ggggatggct cctggctcga ccgaaccttt ggtactggca gcggccttgg ccttgtctcc
     8401 catcgtggca acccagaaaa taaaagccac caacatacag aacaagaacg tgttcagcgc
     8461 catgagcgcg gccgcataac caagcgcggg agaaaacgcg tactgggagg aatcgcgcat
     8521 cacaccgtag atgtgccagt gaacgcggga tgaagaacgc gcatagccca tgagggtcat
     8581 gagcaggatg accatgaccg cgttcaatac cagcgcatag ccggcgcgtg gtggcattct
     8641 tccccacacc atttcggtgg tggtcttggc gctcttgaga aggagcgcag tgagcggagt
     8701 aatcgtcaac atcacaaaga tgacgatacc cacctgggag gtagagaagt aattgatacg
     8761 gataatagcg ggaacgaaat aaccccagac accgagcacg atgactgcta tggctgccaa
     8821 accgagcaca cctcccatca gagccttggc ggccttggcc catcctgcgg tgtcttgctt
     8881 accggcacgc caatacataa tgaagctcat gaatgtgacg agcaccatga gattggaaac
     8941 ggtcattttg gccgacatga cgccgaatac gcccaacaaa ggatggtgcg tgccgcccat
     9001 cttttgggcc tcttccaagc tggcaacgag ggagtgcggg gtcatccaca caccgaggca
     9061 gagcagaaga atgatcaaca tcgccataat cggtttgcga tattgcgttt ccgaccccgg
     9121 aattcgatgg gtgattccga gccagaagta gtagttggag cccaggaaga gcacaccgat
     9181 caacatggcc tgcagaatga acaaccagga gaggaatccg cccatgagcg tgatacccat
     9241 ctgctggttg tactgataaa tttcccgcat cagccagtac ccggcgaatg gaagaggcaa
     9301 gagtccgaac acgccgataa agttcccgac gtatcccatc caatcgtagt gatagcgctc
     9361 ttcctggctc ttggccgaca gataccggat gccggcgtac gcgccacaaa tataaccacc
     9421 gagcaccacg ttggcgatca agcggtgaat gttgaccggc caccaggtcg gattccacgt
     9481 cgccgcccac gcccgcgcga aatcgctgcc ctccgagatc accactggac tggactggaa
     9541 cgtcgcccac gagttgggca cgatcatgat aaagaacgcg aacacgttga ggagaaatcc
     9601 caggaaaatg tgaaacttct tcccgctacc ctcggccatg gcgtcccacc cgtaccagta
     9661 caggtacagc gtggccgttt ccacaaggaa gagaccgcaa tagacgagaa aggacgggaa
     9721 aaagatatcc gtgagataag ccatcaactt cggatagaag ccgatgagca ggaacaacag
     9781 gataccgccg aacagtgccg tcgtggcgta tgaagacgtc aagagcttgg taaactcttt
     9841 ggctagcttg tcataacgct tatcgcctga tttccagccg acgatttcgc acaaccaggc
     9901 aaagatcggc acgccaagca cgaagccggc gagtagcaaa tggagctggg cgataatcca
     9961 gacaaggttt cgactgccga tatacggaat atctcgatac gcggtcggag cctgcggggc
    10021 agcttcctga gcaagggccg catccggcat tcccacccat gccagcagtg ccgcacatcc
    10081 ggccaacagg gccacacggc ctgcgatttg gctcccgtga ttggtgacga tctgaccctg
    10141 gttcatcatt gttctcacct cgtcattcgc catgatgtca tttgccgctt cccgccggtt
    10201 tcccagctgc tgattgcgaa ggcttgccgt cgctcttggg agagccatcc ttgacaccgg
    10261 ctttgtcctt gccgcccttc tgcttgtctt tttcgtcttc tgccgttcgc tgttcgatcg
    10321 cggcaatctt cagctctgcg tccttgaacg ccttctctcc cttagagagg gacttttgag
    10381 catcaaaggg cggagcagcc tccacagtgg caggtttaca acattcgtgc ggatggggag
    10441 tcgacaccgg aagcacggcc cagaatagga gactcaatac tgcgcccgca gcggtgaagg
    10501 cggtcaacat caagctctga tcggcgggca cccggatcat atcccaacgc gatatgagat
    10561 cttgataggt gtctcccgct ggttgggcac cggcagcacg accttcaacc agtttcccaa
    10621 gaatcgacag gccgacaaag gtcagcgcca gaagcacgac gaatgccgca ctgccccaag
    10681 ccgtgaattc cagaccgatt cgctcagcga ccggcatagc cttgagcata tccatatcca
    10741 aaatggattt cgtaattgaa cgggcgagat cggtcgcgcc gccctgtacc acccacaaga
    10801 agggaaggta gatcgccccg gcgaccgcaa atcgaaccca cagcccaatg cccagtgagt
    10861 cggggggctc gagccgcaag cgctccaaaa acacggtacg ggcgaccaag cccaaggacc
    10921 aaatgtcgat tggaatggag agcaggaaca ataagggaag cccgatcccc tccccgaaat
    10981 ggaacccgac acctagggag atcacgaaga atgcaaagac gctgaccgga gtcatgagca
    11041 acatcaggaa tgtaatcccg agtttcgtct ccagatggat cgtccccatc gccataaaca
    11101 acatggcaaa cgccatcttg atcggcaggg cggtccagca ggccggccag agaatgctaa
    11161 gccctaattt aaatgacgac atcgtctctc gaccagtcat gtgcccaaac ccctccgcct
    11221 attccagaaa ttcgcggttg atgattgccg ccacggtgaa aatcagaatg ctcgccatga
    11281 ccacaatcca tccgatcaag gcgatcggtc gcttgaagat attgcgttcc gggctgcgat
    11341 cgatgaacgg caatgccgcg agcaacgcca tgaacgcgcc cggcaaggca atggcgccga
    11401 ggaactgccc gaactctccg gcaaacacct tcagacgcag cagttggaac aagaagagga
    11461 agtaccattc cggttccggg tggtaatctc cgggatccgg cgtggcctct tcgaggagca
    11521 caatcggctc ccagaacgcg aggctacaga tacacaggaa gaccgcgacc atcccgacaa
    11581 tgtctttcca gatttgacgg ggaaagaaat agtcggtctt ggccttaatt tcttccacgc
    11641 tgccccggaa gggtccggca gggccagcct tacggaacag gaacagatgc agtcccgcga
    11701 gccccatcaa ggccgccgga agaaccatga cgtgaatgac gaagaagcga ctcagcgtca
    11761 tctggcctgg cgtcgcgccg cccttcagga agcgcgccat gaagtcgccg agaagcggcg
    11821 tcttgtccat gatctcaaca ccgaccgtcg tggcccagta ggcccgttga tcccacggca
    11881 gtagatagcc ggtaaagccg aatcccatga caatgccgaa gagggccaaa ccgaccaacc
    11941 agatgagttc gcgcggtttc ttgtaggcgc cccagagaaa gacttgggac atatgggcga
    12001 acacgcacac gaccatcgcc gtggatcccc aaaagtgata gctcaacaag aaccagccgt
    12061 aatccaccga gtggatgatg tattgggtgc tcgcataggc atggtcggcc gtcggcacgt
    12121 aatagaacat caacaaaatg ccagtaaccg cctgcatgat gaaaataaag agcaggacgg
    12181 agccaaatac atacgcccat cgcgacccgc ctgggacggg ctcgttgagc attttggctt
    12241 gcagagtctt cagcccgacg cgttcgtcga caaaggcgaa gactttttca atggctgagg
    12301 gctgagtggt cgtcggctgc gtagtcgtat ccatttagac aatccgaacc tgggccttgg
    12361 tgcccgcctt gaattccata tcgataatgg tgatgtcgcc cgctgcagta accttaatcg
    12421 ggagcggatc caacgcacgc ggagccggcc catcaaggac ctttccggct gcgtcataga
    12481 tgctcaagtg acaagggcac agaaagacct ggccgagcgt cttatgcgtt cgccacttga
    12541 aaccgcaccc caggtgagga cacttgccgg aatacgcgat gtagggcacg tctttcttat
    12601 tggtccagat gaccttcccg gatgcgtcat gaaactccat gtctttaccc tgataaatcg
    12661 tctccagcac ctttggagtc gccttcaaaa cccagacgtt tttgtcgatt tcctgctccg
    12721 gcatgtaggc ttctttaatt tttcttttga acttgaactg cacgccgacg tcatcctgct
    12781 tgatctttcc ggaattgccg atcttgagcc attgattgtc gaagggggaa tacatcggct
    12841 tcatcagata gcgcaagaca ggaaacgcaa tcgggagacc gatgagaaat ccgatggcat
    12901 gggtaaagtt cgcaaaaaag gtccgacgtt tgacgcttct ttcaagctca ggaaagggaa
    12961 ccagcacttc ccctggcgtc acgtgaatgc gctcttcaag atgggcgatt tccacctcgt
    13021 gagtcgtcac gtagtcgtca cgcttgaaga ccggaagtgt cgcgtactct gccgaggtgc
    13081 cacgaagcgt cacgacctct tcggcgactc cttgcacctg ccccatgggg acctggcgct
    13141 cgaccgaatc caccgcgccg gaaccgacca cgatatggct aatctcgtgg gagagcgggt
    13201 ccataatgac cctgcgcacc tccccgactt caccgtccgt acatcgaact ttggatttca
    13261 gtttcggctg catgatactc acttcatctt gatgggcttc ggagtattga ccgaagcatt
    13321 cttgtaactg gccaaccaac ccgccagcgc ctgcacatcc ttaggctcca tcaggtcctt
    13381 gtacttgtcg ggcatcttcc ctttttcgta ctccttctcg aagccttcgg ccttataact
    13441 cttcgggtcg agaatctttg tcgcgatttc ctcagccgac aggaggttgc caatgttgtc
    13501 tagttccggg ccacgcttct taccgccttc gccgttcagc ttgtggcagt tgtagcactc
    13561 ctgaagttgc cattgctcct caccgagctt ggcgatatca ccacccgcag cggctgtcgt
    13621 cgccgtcggt gcagcctctc caccggcctt ttgatccgcc ggagcctgcg cagcacccaa
    13681 cgcctgcaaa cgcgccgtca gttctttggc cttttcatcg agcgccttgg cgcgctgcaa
    13741 cagaacctcg actttgccgg cctcgtaatg ttctcgcttc ggcttcaaga tcttgtcgat
    13801 cttgcctttt tcgtcttccg ggtcgaactg cggccacaca gcgccacctg caatataggc
    13861 gactgataga atggcgtaga acacgacgag ggctcccgca gcttttcccc cgctgatcgg
    13921 cttgagcgga gggaggtcga gaataataaa gacgacggcg ccgagcatgg catagccaaa
    13981 aaagagcacc tggaacacaa acggaaatcc cagtgcgttg gtggctccga atagaattcc
    14041 gccgatcacg attccaacta gcgacttctt aatcacgttt cccatgagta ccctcgttta
    14101 tcgcccttcg agtattgtga gcgcttactt agcaccggcc ggcacagggc tcccctcgtg
    14161 cgcatggccc ttcgaatccg aggggcgcaa tgtcacgatg atggcaaagc tgaccacggc
    14221 aaaaaaggtc acggtaatac ctgtaatcca ccaggccgag taagacagcg ttggcgtgaa
    14281 ggactcggcg gtaaaatcag gcagcaggtt gtaggcgtga aaatacttcc gcaacagcga
    14341 tcgtacggca cccatcagcc ccatggtcca aatggcgctg aaggccagga agaccaaaac
    14401 gaactgtgag gcgaaatcga tcttacccca cacaatggtc ccctgcgaaa ccgcacggtt
    14461 gtagatgaca tagttcacca ccgtcacgaa gatcaaggtg aacgcggcgg agtttttcgc
    14521 cggcatcagt gcaaggaaat tccagtcgga tggcaattcc aactccgcaa ccagcttggc
    14581 cccagtgggc acaaaaccgt gcggcgtcat ccagatcgca tttcccacga ctaccatcaa
    14641 gaagccgacc ttgatgaccg tgctggacga tactgtcaga ggtataaatc gccccagaat
    14701 aacgggcgcc aacaggagcg gcacgaggaa cgccagcgat gtcgcatcag gcactggata
    14761 gtcggtcagg accttggtca tgacgatcgg cagcaacacc atcacgatcg gcgcgatcac
    14821 ggtcatacgg acccgctcca ccccctcgat ccgcttcatg ctcaaccaga tatagtaatt
    14881 gctggccaag aaaatcaggc ccaccatggc gccctgcatt tcgaagaaca tcgagagctg
    14941 gtcggccatc atgtacggac aaattgaggc gtcgtagtcg cagagctcat aggacagcaa
    15001 ataacccata aacggcaaga acaagagcgc gccgacaccg atcatgttgc cgacaaaccc
    15061 catccaatcg tagtaggccc gctcttcttc cttcttcgag cccatgaaca tgtaggcggc
    15121 aatcaggccg gcgatgaatc cgccgaacgt cacgttaccg acaagcctat ggagattaag
    15181 cggcatccag ctatagttaa acaccttatc ccacagggaa gcggtcgcca ggaattccgc
    15241 cggcgagaca ccctcggccc tcacaggggt attcatgaag gaggtcggac cgtcgatcac
    15301 gaacagcgtg atcgaaccga tgagattcaa cagcacgccc agcgcgatat ggcgagcctt
    15361 cttctcaccc ttccaggcat cccacgtata aaaatacatg tagaggataa tggtctcgcc
    15421 gataaacaac agcggataca ccaccgcaaa tatcaggaag aagtgattga tgagccaggt
    15481 cgtgaactgt ggataggttg cgagcaacac gaagataaag agaccgcccg tcaatgccgt
    15541 catgctgtag agaatgaccg tgaccttggt gacctctttc gccagccggt catagcgtgg
    15601 atcctgcttg cgatacccca accattccga gatgacgacg aaaatcgggg cccccagaat
    15661 gaatcccgca aagagaatgt ggagctgagc gacgatccat accgccgtgc gattcccggt
    15721 ataagggaac tccacggggg gcgcactggg agcctgggcg tatgccacac ctcccaatat
    15781 cgccactagc gcgaacataa ccaacaaaca gcgctgccag attttcatga tgtctcgccg
    15841 ctcctcctca ttccaaccga ttgacccatg actcgaaccg aacccggcgt gagttacttc
    15901 acctgatcag caggtaccca atccttcttg ttggcatccc agcccttacc tgccaaaccg
    15961 aaggaacgtt caaacgccaa aatcttccaa cgatcctctt cggacaactt gctcttccag
    16021 ggcttcatct tggtgttggg aacgccctca gaaatacgcc agaaccagac cgagtcagaa
    16081 tacaacttca tgcgatcgcc attgcggaag tcccgggcgc ccgccttgac cggcttgccg
    16141 tccttaccat ggcaactggc gcaattgacg tcggggttct tgccgccgat gtacaactcc
    16201 ttaccttctt cgataatttt cggatccgtc caccaaccgg caggcatatg cttgtcggca
    16261 tactccgcgg gagcgggtgg cggcggaact acggggcctt cgcctccccc ttcaccaccg
    16321 ccgcaggcgg cgacgccaaa accgaaccca agaatgccga ccatcagagc caatctcttc
    16381 atgcctgctt gtttcataaa aaagctcccg ctcctttctt catgccggcg ccctctcaag
    16441 caccgacacc tattaccatc gacagaaaaa aaagacgctt ccctgcggcg caacgagcag
    16501 ccacagcaaa agcgtcaaga ctcacaatac aacgaggtca cgtttggact tccccgccta
    16561 cttcttgcta atcgatccgg cgaggaacaa tgccccacgg accgtgccct atttctgccg
    16621 cttcccctgt cccttctttc gccgttgctc ttctcgcatc cgcttgcggg agatctttag
    16681 cccaatccat cccacaatag caacagccgc gatccccagc ccgtacaaca accactgcgg
    16741 aggaccaaga tcgcgctcac acccacttcc gaaatcaacc cgaagcttac gttccgtctc
    16801 cagatcgttc gggtcgaact ggattttata attcttttgc tccccgccga cttcaactaa
    16861 cagcgggtcg cccacattat cattgtgaag atgcagggtg actttatagt acccgtcacc
    16921 atcggtcgtc acagtctggc cgaccgtgat ccgcgtatct tttaccagca cctcaacatt
    16981 ggcgccgggt cgaccgtctt tgccacacac aaaaccttcg acggtaaatc gatggtcggc
    17041 tgcgtggctc gcgaacaaga ccgacggcac aaacaacgtc gagatcaggc cccaaacgac
    17101 aacgacgcgc caggcggttt tccgactaat tgctctccgc catcgcatac gtatgacccg
    17161 tgtcggactt gttttcggcc tcacaaacat cccgcccaca gccatactac gagaggcctc
    17221 atcgacacgg acacgaccca aatttgaggc cgattcttct agcatagccc cccctctttg
    17281 tcaacaaatg gatggagtta aacggaggga gccacgacga aatgcgcgat gaaaattggg
    17341 gacgcgtgaa ggacaattga agccgctggc gcggaacgcg ccggcacaag aaaacgaacg
    17401 acggtcactc gcgcgccagc aagctgacgg ctcacagatg gccgcgggat ttggcttccg
    17461 cagcggcgcg atcgatatcc ttccgtcgtt ccgattcctt ttttgcgctc caggcttccc
    17521 aggacttgcc tgtggccgtg tcctcaccgg tgaaggcgat cgcgaccatc tcagaaaacg
    17581 gaatcgtcag cgttccgctg ctggccgccg gaaagatttc aatggtgggt tgctcacccg
    17641 tcgcggtgcg attaaagagg tatcccgtca catggccccc cgacttcaat tccacggtga
    17701 tatctccccg gtaatcaaat gccagctcga tcgcctctgc cagttccgtg agggaggccg
    17761 gccgaaagac ccggccttca agactgccgg tctccggcgc atggtacttc gcgtcggtca
    17821 taagtatctc ttatcgtcta gagggtgatg cgtgaagcgt tcggccggct cagcgaccga
    17881 accgcttcac cgctgcagga atcacgatat tcttcgcccc gattagcgcg cattcggcaa
    17941 cagggtgatc ttcacggtct tggcaaagcc ggagaacgac ccgaacgtat cctccaccgc
    18001 cgaagcctca tatccgcaat gcaccatgca atccgcgcac ttttcgttgc gcccggtgcc
    18061 ataggaatcc caatccgtct gttccatcaa ctcccggaag ctcgacacat agccctcctg
    18121 cagcaaatag caggggcgct gccagccaaa gacgttgtac gtggggttcc cccagggcgt
    18181 gcattgatac tcgcgcttgc ccatgaggaa ctccaggaac agcggcgact ggttgaactg
    18241 ccagccgcgc ttccgattgc ccaaaatctt ggagaacaat tccgtggtgc gactgcgctt
    18301 gagaaaatgc tgttgatccg gggccttctg atagctatac ccgggcgaga tcatcatacc
    18361 ctcgacgcca agccccatca tttcatcgaa aaacttccga acccgctcgg gattcgcatc
    18421 gtcgaacaac gtcgtgttgg tcgtgacgcg atgaccccgc ttgagcgccg ccttgatcgc
    18481 cttcaccgcc acatcgtaga cgccgtcccg gcaaactgcc aggtcgtgct catcccgcag
    18541 gccgtccatg tgcacactga acgtcagaaa tttcgacggc ggatattcgt ccagtttccg
    18601 ttccatcaaa atcgcattcg tgcacagata gacgtatttc tgacgctcca cgagcccgcg
    18661 cacgatctcg gacatctcgg gatggatcaa cggttcaccg cccggaatac tgatgatggg
    18721 cgccgcgcat tcatcggccg cggcccaaca ttgcgccggg gtcaaccgct tatccaggac
    18781 atgatccgga tattgaatct tgccgcaccc cgcgcacgcg aggttgcaac gaaacagggg
    18841 ctcgagcatg agcacgagcg gataccgttt gaccccccgg agtttctggg tcaacacgta
    18901 cttggtcact gtatacatct gggagactgg aacagccatc cctctctcct attctggtga
    18961 atgtgaatgt aacgtacgac tgagactgcc ggccttctag cagacaaacc acgcggattc
    19021 taccattgcg attgaaaaat cgtcaatttc ggcgggatca accgggcaga ccggcatttc
    19081 ttctggcgcg cgcctccagc ccgtaggcca ggatgatcgc gagacacgcc cccaacagac
    19141 cggctacggc cgtattgaag accacccgcg gcttgatctg cttgtcggcg gggacaggcg
    19201 ggtccagaac ctgaaccgtg ggcgtatcac gagcttcggt aattttcgcc tgctcatatt
    19261 gcgaggtgag caacgcatag agcgtctcct gcaccttcaa ctcacggaac aatcgcccgt
    19321 attgcagggc cagatccggc accgtcacca tagccggatg caggcgatcc cccggcgagg
    19381 cgcccttcgc acccttcccg aactccagca aggccagctg cttcttcaac tcctcgatgc
    19441 tggaacggac ccgtgagaga tcgggattgt ccggggacag gtaggcactc atcacctgaa
    19501 gttgcacctc atagccggtg atctgcccct ggatcatggc cgcagcctcg atcatggcct
    19561 tggactgcgc ctcgacggcc accgctttat tatttcgctg aaagtcccgt aaggcctctt
    19621 cggttttcac cagtcccgtt tgcgtctccg caagacgccg ctcgatgaac gcgcgattat
    19681 aactggcctt gctgacattc aaggtgcggt tcaggcgatc cagactactc acaaagtagg
    19741 ctgcgatatc ggcagcccgc tgaggatccg catcttccac ggtcaccttg atgacttttt
    19801 ccttcgacac agtcacccga acccgctctg ccagttcgac gcgggtatcg tgcatcgtcc
    19861 gctcgtggta caccgccatc aggccgaact tggcaatcgc ctcgtccgcc atcacccgcg
    19921 acttgaggat tgccacaaag acgtcggtag gcgtcgtcgg catgctgggc agccccatcc
    19981 cgagattctg cgccatcccg ccggcaccgg tggtcgccaa cagcgccgcc agactcccgc
    20041 cttctttcga atccagctgc ggcagcaggg tcgcggtcga ttcatacgct ttgggaagga
    20101 ggagggcact gacggcgaag gcacccagca acgaggccgc tataatcgtc gccaccagcc
    20161 cccgacgccg cgagaccacc gacaagagat cctgcggccc catcccttcg cggctcgtca
    20221 ggttcgcccc gtgaccgcgc gaaggagtca tgaccacctg aggactatcc aatacccgac
    20281 tcatgatgtg ctcacgtccc gctcatcgac cgacaaccgc gataccggcg gccgtcagag
    20341 ccacgctgcc gataatactg gccacggtct gccacagggc cagttgtggt gtccgggcct
    20401 cgatcttttg aggcaccacg atggtatctc cggaccgcag ttccttcaca gccaggtagg
    20461 cggagtcggt ggttccgttg gcccgcatga catacatctc tttcttattc gcatcctcgg
    20521 tcagtccccc ggcctgacgc agatagtcct ccaatcccag gctcggccga tagaccaccg
    20581 tactgggatt cttcaccgac ccgatgatac tgaccgtttg cgacggcgtg ggcatcagaa
    20641 tccgatcccg cgcttccagg atgatatcgt cttcagtccc ttcgagttgc tcgatggagt
    20701 ccattcgaat caccacacgc cccaattcca ggcgggaggt gatggcctcc agttgctgga
    20761 gccgcaatga caacacctgt tgctcggcca atcctccgcc agtggataac accgcggccg
    20821 tgctcagtcc ggaggcgcct gccgctcccc ccgccgcctg cgccgtcagc cgttgacgtt
    20881 ccgatgccac aaaccgttcc aactcggcct gttgccggag cttcacggat tcccgcgaca
    20941 ataccagccc ggcgggaaac gcgttctgcg tcaccccgcc ggcacgcttc aagaccgagc
    21001 tgagtcgctc acccatttca atcgtgaaat aacccggtct tttcagctcc ccctcaacca
    21061 gcactagatt gggtgggcga tgctggctgg caacaaccac ctggtccaag cgctggagca
    21121 cgtgattgtc agcctcgctt ccttcgagca gcgcccgggg gctgaattga atcactttgg
    21181 tttgataggt gaccgggtcg gtccgcacga tttcggcacg atcggcatag gcatccatcg
    21241 tgagctgatc gtgaatcaac agatccttca ccttcatgcc ggggcggtat tcgtagggtc
    21301 ccgggctttt cacggccccg acaagactca ccacattggt aatctgcgtc gggagaatgc
    21361 cgatgcgcac atagtccccg tcgagcagaa ccggatcgcc ccctgccaca ccgggccggc
    21421 ttttttcatg cccttgagac gccagcgcgc cgaccagatc cacatccacc atgatccggc
    21481 cacgttcagg atcaagccgg aacagatggc accgctgccg gtcagaaagc ggcgtaaggc
    21541 ctccggcgag agtcaggagt tctgtcaaac gcaccccggg tttcacttcg tagatcgccg
    21601 ggcgcttgat cgatccgctg accgccacca ccggaccgag cggcggaacc aacacgacgt
    21661 ctcccgactg caagcgctga tcgaaacgcc ggtccccccg cagcagaaaa tcatacaggt
    21721 ccaattccgc gacggtcttt ccctcgcgca tcaccttgac ctggcgcaac gatcccgaac
    21781 gggccgggcc gcaggcagca tagagcgcat tcgacgtggt ggccaacgca ctgatttcgt
    21841 aggcgcccgg gcggacgact tcgcccacga catacacttt gatcgtccgg atccgtgcca
    21901 tcgacacgtg caattcgaat cgcttcagaa gattgctcag tcgggaccgc acggtctgct
    21961 cgacctgcga aaaagtctgg ccgccgacag gaatcgctcc cacctgcggg atcacgatca
    22021 tcccgtcacg ttcgaccggc acgatgaagc ttcgattgaa gctggggtcg gggacattcc
    22081 aaatgtgcac ggcaagcgag tcttgcggcc cgacgatgaa atccggcccg accgggatat
    22141 cctggatcgg cgagaaggta ttggcctgcg cttcgaaaaa gtcgtagccg aactgtcgca
    22201 cacggctctt cacgccgtgc aacacagaaa actcggcaaa ggcttcttcc acattgaaat
    22261 cctgctgacg gagcaattca gcggtaaagg ccttcttttc gggcaaggga cgcaccccac
    22321 ccggtgagtt tccaggctga ttccccggct ggccctgagc ttgagcggca ggctgttgaa
    22381 gactgggtac gactccctgc ccgaacggca actgcgagac accctttttc tcacgttcga
    22441 cctgaacctg atactcgata ctcgattttt ccttctcggc ctgcagcttt tcctgccggc
    22501 gatcgcgctc ttcctgttcc tgcttcatcc gctgctcgat ggaactgggc agtaggctgc
    22561 tcggttcgac aggccaataa tcattcaagt tgggaaccgt tcccttcgaa gtgagatcgg
    22621 aggagagagg aatcggacag ggagtttggg tcggcacaat cggctgcaat gcggtggggt
    22681 tggtgacgat ggcctgcccg aaaccgcccc cggccccggg agcgttgaag ccgccgggcg
    22741 tgaacaagct accgggagta ccaggcgccg cctgctgcat cattcccggt ggcgcgacct
    22801 gcggcggaag attttgggcc agcagcgacg ggggcagaat aaaggacagc actaacatcc
    22861 agatcaaggc gtgggagagg gcaacctgac gcacaggggg atggggacgg tgatgcggga
    22921 tcatactcac gaccatggat tctcaatccc tcctgcgcga agcgaagagt cggcatactg
    22981 tcgacacccc tggaggacga cccggcaatc atccggccag gcgatagccg accagccaaa
    23041 ctggtagcct aaaactgcat ggccagagaa gtccagaaca aatggttctg ggcctcgacg
    23101 ccggcagcga acgtttcgac aaacggcgta atctgagaga cttgcccggg attcttgaga
    23161 cgctgaaacg tatagcccac cgtgagctgg gtccggttcg accaccacca ggtgagatcc
    23221 acggccgcct cccgtttctt ctcatggacc ggttgcccgc gatccctctc ctgcaggttg
    23281 aagctcgcgc ccaactgcag tgtgtcgctc agatatcgag tggtgcggac aaaaaagtcg
    23341 gtcccatcgg ttcccatatg gtgcccgagc ggaaacccgc ggtaccgcat accgctggta
    23401 tagggcgaat tgttgtacca gacctgccgt agctcgggat gacggcgcct gccgtaatcg
    23461 gtgtcggcat attcgatacg caaatccagc gtatccccct ggaacacctg tggaatatag
    23521 aggccgccga gaaaggcggt gcggctcgga acgggaagtt gcgaccattt gtcctccgtt
    23581 ccagcctcgc cgtagagctg cagcccggcc ggaaagggaa tcagataggg aatcgaagga
    23641 atccggagcc ggaaatcggc catcgcctgc tcattcacat cccgattgcc ggtttgattg
    23701 ggctccgaga tataggcatc gaacaccact ccagggaatg actggtcccg cccgcggccg
    23761 ccgaactgcg tcagacgagt gagcccgaac tccagccagg aagccggcag gtaactgagc
    23821 cgcaccccga acagtttcgc gcgagggaaa tcccgattct cctccaaccg gcccaggaac
    23881 gcattgacct tccagtcccc gagcccgctg agaaatcccg gcaattgaaa cgggcgttcc
    23941 gttccgagct tgatcatttc catcgggaac gcgtgatcgg tcagcagcaa cgacccgtga
    24001 tagccgggcc cccaccactg cgtcccccgc cccacttcca gcgcgacatt ggcataactc
    24061 aatttgaggc tgaactcgcg caggtagaaa ttttcgctgt tgtttgtcgc acccaatccc
    24121 aagagatggc gattgctgat gaacttcggc tgcaccatga ccgcggccca gtcgctcaac
    24181 tccgcccatg cgcgcacgtc ggtttgattc tgcacgccgt ttacggagta ttccccgcct
    24241 cgattttcgc gaaaccgcac ggtctggccg ccgccgatcg aggaggcatc gaactccgac
    24301 gtgacacggg cgccgaaccg taaggcgccg gttttatcgt cggggcgcgc acggacggta
    24361 cccagacgaa tgagttccgg acggaactcc gcgagcaatc gttccaggag cggttcggca
    24421 atcgcctccc gaccgtccaa ttcgacctga tccgcgcgga ctcgctcgac cgcccgcgcc
    24481 acatactggg ccgcctgcac gcggctgaac ggcttcgcgc cgatcaacgc gcggtcgatc
    24541 acacccaatc cgatcaagcg ctcaatggcg tcataactcc agtggtgcag cggcacatta
    24601 ctggaggcca gggcagtccc gttcatcaag agccacagga ccagaccaag actgagcgca
    24661 catgaacggc gacggatctt cacaaggcag gccggaccgg aacggagcca gagagtgtga
    24721 ggatgagccg tgcggccgga gcctccgtca gagaacggtg gcgatgcatc atttccgcct
    24781 gtgcggagcg gccggcgcaa gaccggcgac cgtttctatg ccggccgggg actgcgcagg
    24841 gatttcggac aggagagcat cggtctctcc tccccgctcc tggctcaaca atgtaccggc
    24901 ggggcaatag gtgggaagga tagcgcagag gagggccagc accttcgaca cattaccctc
    24961 cgcggcagac tcctccagtc tggccacgag cccggataac tgagcgaaat tgcgcgcagg
    25021 gtccggcctg atacagagga ctttctgaac cgcagtcgac tccaccgttt cgtgaccggc
    25081 gaccagttcc tccgtcagtt tctcgcccgg cctgcaaccg agataggtca aggcaatgtc
    25141 tttgtcgggg accagtccgg ataaacgaat cagattgcgc gccatatcga ccacgctgat
    25201 ctgttccccc atctccagca cgaagagctc cccgcccctg gccagacgcg cggcctggag
    25261 caccaggtgc acggcctcgg tcatcagcat gaaatagcgg cgcatgttgg ggtccgtaat
    25321 cgtcaccggc ccgcccgctt tgatctgttc cagaaacagc ggcacgacgc tcccgttgct
    25381 tcccagcaca ttgccgaacc gcaccgtcgt aaaaatggtc cgggacgatc gggccaatcg
    25441 ctgcataagc agttccgcca cccccttgct tgcccccatc acactgacag ggttcacggc
    25501 cttgtcggtc gagatcaaaa tgaaccgctc aacttcatgg cgatgcgccg cctctgcgac
    25561 aattctggtg cctccgacat tattcgacac ggcctcacag ggattcccct ccatgagcgg
    25621 cacatgttta tgcgcggcgg catgaaacac cagcgtcggc cggtactcgg cgaacaaccg
    25681 gttcatctgg ccgacatcgg taatgtctcc gaccaacgga gaaatgaccg tctgcccggc
    25741 ctccttcaac tcgttcgcca cggcaaagag cccgttttcg taccggtcga gaagtaccaa
    25801 tgccgccgga gacaacccgg ccacttgccg gcacaactcc gcaccgatcg atccgccggc
    25861 cccggtcacc agcacacgct ccccctgcac caatgtgcgg agcggctcgg ggtcgagatc
    25921 gacgggaacc cggtcaagca aatcctcgat ggaaagattg cgaatctggc tgacctctac
    25981 ccgacactgc aacagatccc gcaaattcgg cagggtttga atgggaagat gaaacggctc
    26041 cagcgccttg accaccgccc gaaccgcagc gggcttggca ctgggcatcg caacgagcac
    26101 cacgtcggga gcctgccggg cgatgatgtg agacaggcaa tcccggtctc ccaggactct
    26161 caccccatga atgcggtgcc cgactttcgc cggattgtcg tcaacgaacc cgatcggacg
    26221 atagccgtaa gaaggattgt tgcgcatttc tcgcacgatc atggctccgg catcaccggc
    26281 gccgacaatc agcacgcgct tccgccctga cccgaaccat ctcgtttccc gaatgagtcg
    26341 ggggaacagc cgcaatccgg ccagcagaca caacagaagc aaggcatcga tgacaaagat
    26401 cgaggacgga tacgcccgca cacccatccc gaaccggacc accccggcga aggtcagcga
    26461 actcacggca atactcagcg ccagattgcg gagatcccaa agactcgtgt atcgccagag
    26521 accgtcatag agcctgaacg gataaaaagt aagcgcccga acggtcaaca gcagcgggag
    26581 catcgttgcg aacagctccc gttcctgtgc gggaatgccc ccatcgaaac gcaggagaaa
    26641 ggccagccaa ttggacaacg ccgccagccc cacgtggacc acaaaaagca gcaggcgtcg
    26701 ctgaaccgcg gagaacactg tggggaacag gatcggcatg accattcccg agagagaaag
    26761 ttcctacaag ccccgacggg gccattcaag aagatactcc gtctccaatc gccgaatcca
    26821 gccctaggac atgcctcagg gccgattcga gcaaggatgt catcagagga gaggcactcg
    26881 aaggtcaggc tgacctgcca actgcaaaca ggactttgta gccagagact ggttggtttg
    26941 attcaagcgg gacaggctgg gcataagccg ccatgccgtc ttcgaccgcc tcaggaaagg
    27001 ggacgagtgg gccaagaaac agccgtttaa ggaggcgttt cccgtccata gtcgagggaa
    27061 tcagatgcca tgccaccgca gcacgtttga gcccggccac gctggcggcg cgccacgacg
    27121 cagtcgtagc cggaaacgcg ccgtacaggt tgcagtgaaa acccgtctcc ttcagcagga
    27181 ccgacacctg tcgggccgag taataccgat gcgcggagga attgggatta aattccggcc
    27241 attcaggatt gaccatgcaa agcagcagga tcccttgaga aacaagcacg cgccggcact
    27301 cgcgcaagaa tttttcggcg tcttcgaaaa aatagagcgc ctcgaagcag acgatcacgt
    27361 cgcgggacgc cgcgcgaagc ggcaaggctc cggcctgcag tcgcaccaac ggtatcacgc
    27421 cccgcagact gcgccgggcc tggccaagca agctgccggt gagatctccg cccacgatct
    27481 cgccggcatg ccgggccaga taccccagcc ccacgcccgg accacaggcg acttccaaca
    27541 cccggcgccg tcgacagaaa tccgcagcga agcgatacct cgtgtagacc atcgacaacg
    27601 cctcttgcgt cacccggtct ccggattttt cggtcacggc actgtagtcg agagcgctca
    27661 cggccgcttg gagaagaacg attgcaggca ggcaacagtg atggcgacat ggtcgtccgt
    27721 ggtctccgcg ctcatcggca acgacagaac ctcccggcag agcgccgcgg tttcgggcac
    27781 cgacggagta accaggccca agccccggtg ctcccacatc ggtttcgccc aatggaccag
    27841 tgtttcgatg ccctgctctt taaggtaggc ccgcagccgg tcgcggacat ccgtacgaat
    27901 cacgtaattc tgatagatgt cgtgatggcg ggtctgatcg aaatgtggca agcgtaattc
    27961 tctgatcccg gagagcccct gccgatatag ttctgagatc cgccgccggt gagcgatcca
    28021 tgcaggcaga tggcgcaact tcacatccag gaccgcggcc tgcacgttgt ccaggagcgc
    28081 ggtctggcca tggcagtggt actcgcccgt ttcacgatcc tcgccgttgt agcgcgagcg
    28141 cgtagccatg cgcgcaaggt ccggatcgtt cgtcgtgatc gctccgccat cgccgaatcc
    28201 cccgagcacc ttgaagggat agaaactaaa acagccggca agcccctgac tcccggcgcg
    28261 tcgaccgtcg tacgttgcgc cgagggcctg ggccgcatcc tcgaccacgg ccagcccgtg
    28321 cctgtcggca atcgctagca tcctgtccat ctcgcacacc cggccgttga gatgaaccgg
    28381 caatagggct ttggttttcg gcgtgatcgc gcgttcgatc agatcggcat ccatgttgtg
    28441 atcgggcccc acgtcgatca gcaccgggtg cgccccgcaa tgcacgatgg cggaaaccgt
    28501 cgccacgaac gtatgggcca cggtgatgac ctcgtggcct ggcccgacgc ctgcgccgag
    28561 aagggcgaac agcagcgcat gatagccgct gttgacgccg acggcatatt tcacgcccac
    28621 gaattcggcc aggtgctgct cgaaatcctt gagctggtgc cgattgacca gatcgccgtt
    28681 cgccaggcac cccgtgatcg cgctgtcaat ctccggctta agtttcgcgt agtgactgcg
    28741 cggatcgatg aacggaacct tatacgccat aagaattcac ttgctcccta gaggaggact
    28801 ttttctacgc tcgccacaat atcctcggcg ccgacataat aggctcgttc gagcgcggca
    28861 gaggtggggg ctggaacatc cgggaggcag acgcggccga tcggggcctt caacgcatga
    28921 aagatgtcgc cggcgaccgc cgccgcgatg tccgccgcga tccccccgct catccatgcc
    28981 gcatcggcga tgaccagccg gccggttttt cccacggaag aatatacgag atcacgatcc
    29041 cagggtttaa tcgtccgcaa atcgatcaac tccacgtcga tgcctcgctg cgccagcgtc
    29101 tccgcggcct tgagcgcctc agatgccatg tacgacgtcg caatgatcgt cacctctttt
    29161 ccgctgcgac ggatcgcggc cgacccaatc ggcacctcgt aaagcccttc cggcacatcc
    29221 ccttccgatt catacagcca gcgatcgtca aggtagagga ccggatttcc gtcccgcacg
    29281 gccgcaacca gcagcccctt ggcgtcgtag gaggtggcag gcatgacgac cttcaaaccg
    29341 ggaatatgcg caaacagcgc ctgcaacgat tgtgaatgct gtgcgccctg ctcgccgccg
    29401 cggttgacga ccgcgcgaat gacgaccggc acactcacct gcccgccgaa cagatacgac
    29461 caattggccg cctgattcac gatcgggtcg gctgcgagaa gcatgaaatc catccgggca
    29521 tgaaatacga tcgggcgcat gccgacaatc gccgccccga tcgccgcgcc ggtcgtcgca
    29581 ttttcggaaa ccggacagtc gaacacccgc tcacggccga attccttgtc caggccgagc
    29641 aggctcttgc ccgcatacca gggattccag acgccctgcc cgcagacaaa cacccgcgga
    29701 tcatgccgca agagttccgc atgagcttct cgtatcgctt gcgcatagct acactggcgc
    29761 atagggcaac taggttctcc gtggcgcata gacatgcctg aacagatcgc tgtcgtcggg
    29821 cagaggcgat tcgcgggcaa atgtcacggc cgcctcgatt tcgccccgga tggcgggctc
    29881 cagcgcgtcc agttcgttcg tcgactggcc ctcttcgatc aaccgacgtc tcaaacgcag
    29941 gatgggatcc ttgggcatcc attcatccag ctcgctgcgg cgacgctctc cgacttccag
    30001 gtcccaggcc gggccgacat gaccgcgcca ccggtaggtc cggcattcta aaagacaggg
    30061 ccccgcgccg gccctcgcct gcgccacggc ctgtctcgtg ccgtcgagca ctgccagcac
    30121 atcgttcccg tccagacgaa tgccgggcat gcccgcggca tcggcggcct gaacgatgtt
    30181 gtccttcaga cgacgagccg acagcggcat atgcgtggaa taaaaattat tctcacacac
    30241 gaatagtacg gggagacgat agagcgcagc cagattcagg gactcgtgaa aatgcccctc
    30301 ctccgtcgca ccgtcgccga aaaaggccac ggccacgtgc ggctcgtgac gaagcttgga
    30361 cgccagcccg gcgcccaccg ccaaaggaat ggtcgccccg accagcggga ccgtcccgta
    30421 caacccctgc gcgggagcga cgagatgcat ggatccgcct cgccccttcg agcagccggt
    30481 cacccggccg aacacttccg ccatcatctc cggcaaatct ccacccttcg ccagatagtg
    30541 gccatgcgag cgatgccccc cccacaccgt atcctgccga ctcaatgccg cgcagacgcc
    30601 ggcggcgatc gcctcctgcc cgatggagag atggcacgga gtcttgatct ctcccgcaga
    30661 gactaattca ccgatccgct cttcaacgag ccggatcagc aacatcattc gcaacaggga
    30721 tcgactggcc gctgcggcac caccgacttg tatggttgca tccacgcgac taccccttct
    30781 ccggcacgaa catgaccccg tgtgaccgtt tcccgccgac cagcgcaacc agcgtatgcc
    30841 taagaatttg cagatccgtc cgaaagctgc gccgacgcac atactccaat tgcaggcgaa
    30901 tcttcaccgg gcggatcgtt tccaggtaag tcttgtccgg atcgggactt ccctgcagca
    30961 atgccccttc atcgatattc cacaagctcg cccagtccgt caacccgggt cgcaccgcca
    31021 gaatggcgca ctcctcgtca gtgtagagat caacatactg ttgcacctcc ggtcgggggc
    31081 ccaccaggct catatctcca atgcatacat tgaacaactg cggcaactca tccaacttat
    31141 accgtcgcaa ccaacggccg atgggggtca cgcgtggatc gtcggcaggc gtcgagggac
    31201 cgccgagcgc atcggcccgt tccagcatcg tcctgaattt gcacatgcga aacgatctcc
    31261 cccccagccc aacacgaggg ccgcgataca acaccgcgcc gccatcgccg agtttgacga
    31321 gcatagccag caccagcagc agcgggctca gcaacagcag agcacccgag gccaccacaa
    31381 gatcgaagaa gcgcttcatg acgtacacat ctccattggc cgcatgaaca tcagagctcg
    31441 accgcagatg cacctccggc gcctgcgagc ctcagttttc tccagaaccg gggcagactg
    31501 atcaaccgca cgagccccca cagcgacccc agcccatacc ccagatgcag cgccgcaaag
    31561 acgaacggca tcaggataag gcgggagaag gacttgctgg tccaagccac atgaacagaa
    31621 gccgctacgt tcaccatgcc atacaccgca gggatcacgc cactcaccca acaccagggt
    31681 cgtgatacga cacccacggc ggccgtcgcc agcagggccg tcacaaagaa caacggaatg
    31741 agatgacgaa gggagaccgg cacgccgtca gtataggcga agggcaacat ggcccacagg
    31801 ccgttgttcc aattctgtcg ccagaacgcg gatatggttg aacgggcata atattcactt
    31861 gccacatcag gcaccagcaa catgcgcccg cccgttttcc gcaagcggag actgaaatcc
    31921 atatcctgcc cgcggatcag gtccacgtta aacggacctt ggcgatcgat cgatgcgggt
    31981 gcgaggggca taaccccgct cgcacgcgaa ccctccggtg ccggaacacg cgcgaaaacc
    32041 tcccttcgat aacagccgca aaacaccgta tcgacccacg tcgcatcgga cacgtgaatg
    32101 cggaaatatg agttccccac tccaaacgga tggctcaacg cggcagcaat cgcctgaccg
    32161 accggagaat ccaccgcggg aacggtccgc atcaggccgc aggccacatc ggcatgatgc
    32221 tcgtacagcg cctcgacgca ccgccggatg tagccccgct ccaggcgcgc atgcgcatcc
    32281 acacggagga ccacgtcccc ctgtgcttcg cgcacaccga gattgagggc ttttggcgtg
    32341 gtcctgccgg ggttatcgat cacccggaca caggcatggc gttgcgcata cgcccccagc
    32401 acggaacgag ttccatcctc gctcctgccg tcgacgacca ggacgccgag gcgatcggca
    32461 ggatatccgt tgctcagaat cgacgtcaga cagcgctcga taaagtccgc ctcattccgg
    32521 catggaacca ggactgtcac gaacggcaat ctgtatcccg gctccatctc cgtctactcc
    32581 gtaggcgccg gagcggcagc catcgacaag ggggcgaaca ggcgggcaag cagaccgctt
    32641 ccaaacgagg cgatgctcac aacgagcatc ggcaccaaca gcccttcgat caccaggcct
    32701 ttataccctt caaggcccgc tcgaatcagg ggaggaatat aggcgacaaa tccccatggc
    32761 gccatgaccg caaaggcgta ccgccatcgc ccccctcgat cccgcgtagc ctccgcacag
    32821 aggtatgcat taaaacaccg gccccagatc catccccata aaaccgcgcc gaacagcaat
    32881 cctggcaggc cgaaatttag ataccagccg gttgcatggt gaatgctaaa gccctgctcg
    32941 cccacacctt caacaatgcc gacgctcgat gcatagtgcg aataaatatc cccaggcctt
    33001 tccggccaga gaatgcgggg cacgaccgaa aacaacagcg ccgtcaaact tgaaccatag
    33061 gtcaacggcg aatggaaata caacgcgcca tagagagaca gatgagcgcc gaaggcttcg
    33121 ttgctcgcgt gaatttcagg cacccactgt atcgcgtccc accaggcgcc cccttcccag
    33181 aggtcgatga gaggaaaggc ccgcaaaaaa tccacgagtc cgatgcccat gaacatcatt
    33241 ccggctccgg cgcccacgaa tccccacttc acccttcgcg tatttgcgag atggaaaaag
    33301 ccgccgagga tcagtgccga gaagagctcg ttcttgttcc cgagcgcggt cacatagagg
    33361 aacatgccgc ccagcagcac actatacccg gcgccggtta ccaacgtggc gttgccggca
    33421 agccagcgcc cctggcgccc ggtgaaccag actccaattc caatggcgag agaaaacagt
    33481 gccacccgat tgagcacttg atgaagtgta aacaagggca ccatttcccc cagcccgccg
    33541 cgcgtcacgg aataacccga cttccccagc agcacggccg tcgccaactg atccttcacg
    33601 atgagaaagc tggttccggc cgcgagtgcg gcgatgacga ggaggaccgt atgcgaaatc
    33661 cacaaggctt ccgcagcctg gtccatgtgg ggacgatccg acctcactac ggcaaggagc
    33721 gtcaactcga tcaccaggac gaacagggca tacagcagca gagtccaaag gtactcctcg
    33781 tttaattcca ccggaaatac tttccagtac aaatagtggt gttgctgctg gctgtccgcg
    33841 cccagcccat cggcgacgat cgaccagccc ccgtagagag accagaaata taaaaacgcg
    33901 atgcctagcg gaaacgagag gctgaccgtt cttgcataga gcaaggcggc tagaccggcg
    33961 atccctaccg ttgaggcggc gagcacaaga taaaattcca tccggcacat cctctaatcg
    34021 ggcgaaatcg ggttttgtgc ctcacgacct tgctgagtcg acgaggcagg caccggctcc
    34081 aggcgcccgc gctttgtgac gagccaccga tcgatcacca gatagtcgag gccggagcgc
    34141 agcagcacct ccaccgcatc gcaagccccg gcgacgatgg ggaatccatg aagattgaac
    34201 gacgtattga gcaacacgta ccggcccgtc atccgcccga aggcctcgat gagcgcgtgg
    34261 aaggacggat tgagatcctt ccagaccgtc tgcaccctcg ccgtatgatc cgccggatgc
    34321 acggcggcaa tgaagtctgc gcggtgccag gtggtttcga cggcatgcat catgtaggga
    34381 ctgacgcgcg gccttggcag cgccgacgga atgcggacat accgttcagc ctgctccccc
    34441 agcaccgccg gcgcaaacgg catccaaaag tcccgctgtt tgatcatacg gttgatcacg
    34501 ttcaccaccc ccggattgcc gggatccgca aggatggagc ggttcccgag cgctctcgca
    34561 ccgaactcca tccgtccgga gcaacgagcc acaatgtggc cctgtgccag cagttcagcc
    34621 gtacgctcct ccggatcatc cagttcctga aactccgcag cctcaccgta cctggctcgc
    34681 gcttccgcca ggcccgcacc tgcctcaggc ccgacataga tattgtcgaa gtgaatatcg
    34741 tccagcgctc cgcccccgcg cagatgacat tgccaaacgg cgccgaacgg caacgtttcg
    34801 tcgccacagg acggaaacac atcgaaatac tcgagcgcgg gcagttcggc gatccggtga
    34861 ttggccttca cattcataaa cactccgccc gccgccagca ccttgcgcac cccggtccgt
    34921 tccatggcgg cagccaccca tcggcacatc agatcctcgg taaagagttg cacccctgcg
    34981 gccatgaggt cgaaacgcac cgctcgaaga tcatccatca gcctgggcag aatggcgctc
    35041 gtccgctcga gtgtctttcg acggaaacac aaaggattct ggggatcgag atccaagtac
    35101 cgggcaaacg agttcgccac ctcgcgaccc cgctcccctc cggcataagg agccaggccc
    35161 atcaccttat actcgtgctc atggggacgc atgccgagca ggtacgtcac cgacgcatag
    35221 atgttcccca ccgaatggcc ggtgggtgtt gaagccagca atcgcaactc cccgtgctga
    35281 gccaggtaga cctgggcgca ggtgttatcg ccgccgccgt ccaaggtcaa taccagatgg
    35341 ggttcctccc agttcttgcg gagcgcgtaa taggccgacg cggcatggtt cgaatggtga
    35401 tgaaaccggc tgaccggaat gccatcgagg ccgcactgcg ccaattgtcg ctcaatcacg
    35461 ggatgactcg ttgccgtccg ccgcgagact tgccacctcc gtcccaggtc ctcgatcgaa
    35521 gccggtacaa ggcccgcgca tctggaagcg gcgccggacc agtcaccccc gagaacgagg
    35581 tctttccatg ggcgtgagct ccgggcgtaa taccggagta ggtcttcacg agtttccttc
    35641 acagggctgt tgagattgct tagacacaca tgatcgaggc gtcgagcatc caagccatac
    35701 gctttcacgc aagccatcac cgcctggagg ggaaacccga tggctccctt ctcccgactt
    35761 acccgttctt cctgcagggc gaagaccact cgtccgtccc ggcacagcac aacggaggca
    35821 tcgatgcctt cattgatgcc cagaatcagc atgccgccct cttcgacccc agcacctcac
    35881 gatagagatc gaccgttttc ctgacaatgt gatcctgggc aacgggggcg gtccacgcac
    35941 ggccattgga gcgcacagga cgagccagca tctcggccaa tcccttcccc agcgcttgcg
    36001 gattccgttc gacgattctg gacgggacta ctccggccag gcgttcccgg acatctccga
    36061 ctccgaccga cagaaccggc agattacagg ccaatgcctc ttggacgatc gtcggagacc
    36121 cctcccaatc gctggtgaga agcaggcaat cggcgccgtt caacatggtt gcgactgtcg
    36181 aatgcggcac atgtccatct aatgtcacga accggatcgg gccacattcc ttcactgcct
    36241 cgttcaccgc cgcctgcgca agatccaacc gtttcaccac cggtgagagc cccgcattga
    36301 acagcacgat gcgatcctcc tcggcccatc cgagttcctg tcgggcgtca gaccgggatc
    36361 ggggaacaaa gagattcgtg tccaccccgg acggcaagac aaccgcgcgg tctctagccc
    36421 accataaccg gcgcgccaga ccttcgctga cacagatgat ccgggccgcc ttgcgcgcag
    36481 catactgaga caaccagcgg cctagcgcgg accggagcca gggatcgctc ggagcgggat
    36541 taagatcgct cccgcgaaag gtgatcacca gaggcaccgg cgtgatcagg ctggcgaaaa
    36601 acgccgtcat ggtcccatac tgcgcatgca cgagatccgg gcgaaaagaa tccatcaggc
    36661 cacgtagcct tgcgtactcc cggcgcaaac tgcgcaaatc cgtccgggac gccagacaga
    36721 acgtttccgc cgccacaccg gcctcccgca ccgcctcgac ttgtttcttc gcaaaaatca
    36781 tcgccgaacc ctcggcttcg cccggaaaca ggtagagcac ccgaatgggg cggccttgca
    36841 ggggtgccga gtccctctgc cgatcctcgt acgacacggc cattccgacc atatctgaca
    36901 tcgagcctcg attcacggcc caaccgaacg aaaggtctct cgatacacct tcgactgttg
    36961 caccgcccgc gcaaagacct catgcccggt ggcattccaa tggttgtcgt cgggaaactc
    37021 gaacgtgagc tgccgctgcc tgtaatccgc gatgaatgcc ggctgcatgt cgatcacctc
    37081 gaaaccctgc tgcctggcgc gatccatgaa atgcattcgc atcatggcag catagacatc
    37141 cccaacggac gccagccgat cggtatcgta gagttcaggc cggatcccat caacggccag
    37201 cgcgatgcga ccgggtggca atcccgacat ttccggcaga cgctggagaa acgcctcgat
    37261 cgcccggtcg gaatcggcga ccctggccgg cgaggcctcc accgcggtat ttccgacgaa
    37321 ccgcggccgg ctgatggccg actgtcccgg ccgataggcc catcgcttcc acacctctcg
    37381 aatttccaga ttcacataca aatacatccc gagtgaagac ccggccaaaa aatcccgcca
    37441 ccagggcacc ggtcggtcga tgcgcaccag gtcgagccgc ccgtccggcg actccgcaaa
    37501 gtaatgcaac cccggctcgc tcttatattt acgcagactt tcatcgaaat cgttgccgat
    37561 gatcaccacc gtcaacccgt cgggacggaa tgtgtccctg acatattgcg cataggccag
    37621 atactggctc agggaggacc ccgacgtccc gaatccatac acacgcccgg cagagccgac
    37681 ggtaccggcg agcctcgcca cccccgtctg gggaaacggg accatgacag cctccacaaa
    37741 actatctccg atgacggcca atagcggcgt cttggccgac gggtcgtact cctgatcgtt
    37801 cacaaaccca taattgttgg ttctcactcg attgacgatc gaaaagttcc aaccccgcga
    37861 atacaccgac tcccgatggg aggtaaaacg aaacacggga ttctgctcat ccacggagag
    37921 gcctcgaagt ccctcgttga ccggaagcca ccggcagatg acttccagaa acagtgccgc
    37981 cacgaccagg gacacgagca tggacgcgag cgccaacacc acccgccgcc tgccccgtcg
    38041 cggcgggtgc acgcttgtca tcggcgcctc tccgcccatg gatgaatatc gataagtcat
    38101 gaacgtggca gcggggtcgc aacgatgcaa agcctcgcca ccccttccgt tagcgagacc
    38161 gatttgacgg ctcgccggac acacagcgcc cgaccggccc ggaggagcat tcacgaagcc
    38221 gcggcaacat tcgacggtag aaggccgtgg ccagcagcat gccggccaac gcatactgga
    38281 ggctgatcgc cacggcgatg cccagcagcc cgcccgccag aaagccgagg gccttcaagg
    38341 cgaggccccc gagactggcc acagcctcga ccctggcggg aatgcttgtt tcgccttcgg
    38401 cataaaacgc attgaccagc agatgattca ctcccccgca caacagcccc ccggcgcagg
    38461 cgaagaatgt catccggaga gtcgccatct cctgacttgt aattcctccc tggcccggct
    38521 gtcccccgga ccataatccg atactctgcc cgaaaattgc cactgtcgac agaccgacga
    38581 aggcgagcgc gctgagtcct gccaccccaa ccagccttgc tcgacagagc gccacaaatt
    38641 cctcccaagc gcgagcctgc gccagtttcg ccagggtcgg cacgaccggc gtgacaaatc
    38701 cctgattgaa gatgcgaacg accgcggcca gtacccgcca ggtcagctca agcatcacca
    38761 ggctgcctgc gcctaggaag gaagtcagaa aacgatccgc gacgaatccg gttcggacaa
    38821 aggccgcgct caacacaagc ggtcgcatac gcctccccac ctcacgtagg cacgtgatcg
    38881 cgccgggaaa cgaaccaggc acccatgctc ctaatgcctt caacagcaat agcgccggcc
    38941 cgctgagcac gaagacctgg acccaggccg caagcctcac cccaccactc gacagcgcct
    39001 tgactaagac ccaccatcca agcagcgaac agaggaggag cgcgccggct gtccagatga
    39061 accggtccct ggcctgatag aacgcggaca acagcgtgaa acaggccacc ccgatgagcg
    39121 cagtcagttg gattcgtgtc agatcgactg tgaggtgcgt ggtcgcttcc gaaaatccgg
    39181 gaaccatcac acgcaccaaa tacggcgccg cgtagtacaa caccagcgtc acaagggcaa
    39241 cgagtgcccc gactcccagg aacaacggcc aggccaaccg ccgtcgttca tcctcgggac
    39301 gagcggacag cagcggcacc agcacaaacg acagcgggtc catcaccacg accgacaaca
    39361 gctgcagcaa cgtcgtcccc gcgtataacg cgtcggtatc aggcccggcc cccaagtggg
    39421 cgaccgtata ccactgaatg ccgaacgagg acagcagttg ggcggcggtc agcagggaca
    39481 ggcccattcc cagccccaga gcgttacgcc ccatacggaa aatcctctcc agcggtccat
    39541 caccgggtct acgccggcgc tcggcggttc gtggcccata tcacgccatc gccggaatca
    39601 agtcaaggtg tcgcagcccg ggtcgcttcc gcgttgacgg atcatgccgg gcccacttat
    39661 gattcgctcc cattgtgctg caggtaagct ccttcacatg gcgtgctctc acgctggtcg
    39721 tcatttgcac ccccctgctg ctgcttcacg cccgcatggc ctcagcagac agtccgacgt
    39781 cgtccgcctc cgcctctcac gatctggacc ttcgactcga aacgacgacg tatgtgctca
    39841 accttgaaaa gcaggggctg gaagacaaca ccttcaccaa cggattgggg cgcgacacca
    39901 cgctcatcgg caacttactg aatgtgtatg tacgcaaacg cctgttcgcg tccatggacc
    39961 tggacctcgg cctgttcgcc aacatgccgt tcggccacga cacggaggtg tcgcaggtcc
    40021 ggccgattat ccgccttcag tatcgcccaa cggacgaaat caccaccgtg ctcggcaccc
    40081 tggcgggccc acaccgggac tttcacgatg cggtcttcga caacgccaac cggtttgtgc
    40141 ggcccctcga acagggcgca caagtcctgg tgaactcacg ctattaccgg caggacctgt
    40201 tcatcaattg ggagcaggcg tttcgcggct ccgcgaccaa ccggtacgat gtcgggtatg
    40261 ccgggcaatt gcgcgcgggc cccttccgtt tcaacggcca agcccattgg gtccacaacg
    40321 gccaagccct cctgaaactg gaccggtcgt tcaacacggc cgacaatctc gtgaccgccc
    40381 tgggtcctga actagtggtg gagccatcaa cctatttccc ggcgctcacc tggtggcggc
    40441 aggcgggtat tcgcgccacc tacctgacct cgctgaatga gccgctcgcc ggtggcccag
    40501 ccattcgggg acgcgggtat gaattgagtg tctggatgga cttctccgga tggaggccgt
    40561 cggtctcctt ctggaagggt cggcacttcc taagccaaca gggcgatcct gaatatgccg
    40621 cgagcaactt tccggagttc ggcctcgcga aaatcattcc gcttggcgaa tcggcctcca
    40681 tcgagttcgg tgtccaggcg cgccgcatcc gggccttctt cacggaagac ggcatcacgt
    40741 atcagaaccg tgacacattc aagtgggtca gccagcaata tctggtcttc aattggaatt
    40801 gggacaccac ggacgggcaa ttcgtgcgcg atctcttctc cgccggggac actccctcga
    40861 gcgaaccgaa accgacacat ttgccgtctc gatttacggc caagttcgat gccctgacct
    40921 atgtatacaa catcggctat tcaggccttc ggcaaatcgg agggcgaccg gtcgcgaaca
    40981 gtaccctcgc cggtcaatac cttgcgccgg tactcagata cagcccttcg tccaggttga
    41041 atctggatat cggcgtcttt gcggggttgc ccgtcgggga tacgcagcga ttccacacgg
    41101 tgcagccgat cttgtcggcg gagtatgaac tctggccggc ggtgtcgctg attgccggca
    41161 caatcaagcg taatcacccc ttggtggatg cgctgttcaa cgacgcctcc ctgttttcac
    41221 gccctctcga acaagggttc caactcctgg tcaaccggga acattaccag caggatctgt
    41281 tcatcaactg gaaccagatc gagacgtttc aaaaggcgga gcgattcgac gtgggatacg
    41341 cagggcgcgc gagcgccgga ttcttttcct tgaacggcca ggtctactgg acacacagcg
    41401 gcggggccca gtactcggaa tcccgcacgt ttttcggagt cggcctgccg cgagaccgac
    41461 cggcctcgaa caatttcctg acggccgtcg gaccgcaatt tacgttccag cccaatcgct
    41521 attcgtcggc cctgtcgtgg tttcgcgaaa tcgatgtgat ggcgctgtat ctcaccagcc
    41581 aaaacgaacc gacgcagagt ggagcgccga tcgttcgcgg ccggggctat cagctgacgg
    41641 ccggtgtgga tctagacggc tggcggccct acgtcacgat ctggcgaggc gagcatttcg
    41701 tgaatgaaca gggcgaccca gcctacttcg ccggaaactt cacggagttc ggactgttgc
    41761 gtgatgtcct cctgccggcc ggattctcct tgcgggtcgg cgggttcggg cgcgtgatcg
    41821 aagaccgcgt gacccatacc gagtacgcgt tactgaactg gtcctgggat caatcgccgt
    41881 ggcgcgggtt ctgcctgcgg ccgacattac tgcatcgaag cgaatcgtcc tgcagccggg
    41941 attaagacac acttctctct tgccaccccg caccaagccc gttagagtag ccttcagtga
    42001 aagacacggg atagtcgttc agttggaggc gctatgttcc gcagatggat cggtactgtg
    42061 ttgggaattg ttgtcatgta cgcgccggcg ctcgtccatg ccgaaaacta tgccgccttc
    42121 gcggtcggcc cgaatgtggc aaccctggac accacggacg ccggaaaact cgacctcaac
    42181 accaatctgg cgttcggcgc gaaatacgga cattacttcg atgaccgtgg attcaactgg
    42241 ttcgggctcg aggtcgacgt ctatcgctct cagtcgaacg tcaaggccca aactcccccg
    42301 cggcagggta ccaatctgct tctctcggga ccgattccgg gaagtgactt cctggttcac
    42361 tcccttgcgt ttaatgcact ggttcgcgtc accggctatc agtacaaagt ggagccctac
    42421 gccgggcttg gcctcgggct caatctgggg aatatttcgg acgggaactt ccgaccggaa
    42481 gcgtcgtttg ctcccagttt caacgtgctg gcaggcatca aatattatct caccgcaaaa
    42541 gtcgcgccgt tcatcgaata caaatataac ttcgcgcact tctccttcga ccgaaccaac
    42601 gtcacggccg actatcgcgc caacctcttc atgttcggcg tcgcctacca cttcgatcgg
    42661 tagacacgcc gggccctgga atcctgtcta ttgaaggggt atcctgtgtt acctgccgtc
    42721 gtcggccccc cctctaccca cgcacgcgcc gggcaacgat cgacatgacc aacatcccga
    42781 acgcctgcta tctttcgctg gacgtggaag actatacaca cgccactatg ctcgacatgg
    42841 gacttcggcc gcggactaat ccggaacaga cctggagagg gctccgccgg attctctcgg
    42901 tgctccgtca agccggcggc caccccaaca tgacgctgtt caccaccggc cagattgcac
    42961 gagatcagcc cgatctcatc cgtgaaatga gccggctcgg ccatgagatc gcctgccatg
    43021 gcgatcaaca tgaaaatgtc tgtacgctga gccggacgga gttcgcaggg aatgtccgtc
    43081 gcgccaagga tgcactcgaa gacgcctgcg gacaacgggt gatcggattc agagcgccga
    43141 acttttccct cgacgaacgg tctacctggg cctatgccgt tttgtcggag gcggggttcc
    43201 tgtacgactc cagtcttgtc atggaagccc cccgcgtctc gcgccaaccc tatgacgtct
    43261 tcgagctgtc aggagcccgg ctgtacgagt tcccgttgta tcggtatcca gttgtcggca
    43321 ctcgtggtat ccgggtgatc ggcgggacct actttcgatg cttgccgttg tcggccatca
    43381 tcgcgctcat gcgccaggcc ctcaggctcg gatacacgcc gttggtctac ctgcatgctg
    43441 cggatgcgga cgatcagccc agccccgtga cctggaagga catgcaggga ctgtcctatc
    43501 tgtcgcgcag ccgctggacc atccgtcaaa aacaatggat gctgggcacc catgccgtcg
    43561 cggacaagct gcttgcgctg ctcaagctct ttcctcacct tggaccgatg agtacgacac
    43621 tgccgatgaa ggacaattcc cgattgaacg cgtccgacta gaccctgagc gccattcata
    43681 cactgaatca ccatgtacat tctcggatta gccaccatgg gcgagagcgc cgcagccctc
    43741 atgaaagacg gtgtgcttgt cgcggctgtt gaagaggagc gcttcacgcg cgtgaagcac
    43801 gaagggtgtt ttcccctgcg tgccatcacc tcctgcctga atcgagccgg catcagcctg
    43861 gcggaggtcg cacatatcgg cgtctattgg cagccgcttc gcatgggtgt ccgtaccaaa
    43921 gcggtcttga aaacagctct cacgcacccc acagcgtttc tcggaaagat gggtcgggct
    43981 ctgcaggaat tcacgcctgc gggcggcgga caagcagtgg caagtcaggg cagctgggcc
    44041 gaattgtttc tggttcgctc cctcctcaga aaacacttcg gggccttcaa ggcccgtctt
    44101 cattacttgg atcatcatcg ctgccatttg gccagcgcct tcttcgcctc gccgttcgaa
    44161 gaggcgacgc tcctggtctt cgatggtgca ggggaagatg tgtccacgac tctggccgtc
    44221 ggcgccggaa ccgacctccg cgtcctgcgc tcgatcccct ggccgcattc actcggccat
    44281 ttctattcag ccatgacggg atttctggga ttcgccatgc tggacggcga gtacaaaatg
    44341 atggggttgg ccccatacgg cagcccggac tatctgccct tcatccggca aaacatttta
    44401 ctcaccgacg cgcccggttc ctatcggctc aacggggatc tgctcgacta ccatgcggcg
    44461 ctcaagaaac aatttcaccc gaagctgctg gaggcattcg gcagccccag attgagcgac
    44521 gatgccccgt ttaccgaacg gcatcgacag attgccgcga gtgcccaggc cgcgttcgag
    44581 gaagtggtcg gcgacatggc ctcatgggcg ttccagcagg gagggaagcg aaccaatgtg
    44641 gcgatcgccg gcggctgcgg cctcaattgc tccgccaacg ggaaactgct tcgcgacgga
    44701 ccgtttaccc gaatgtacgt tcccccggcg ccgcatgacg ccggctgcgc ggtcggcgcg
    44761 gcgctgctcc tgcaccatga gcgattggga aagccgcgac agttcgagat gacgcatgcc
    44821 tattatggcc cggcatatgg agagcaggat atcggccggg ccctgtgcgc actgggcctc
    44881 gaggcgcggc cgatggctga cgaagaggcc ctgcttcaac gtgccgttca ggcactctgt
    44941 tcaggccaaa tcctagcctg gtttcagggg cccatggagt tcggcccgcg cgcactgggg
    45001 tcccgatcgt ttctagccga tccgcgccat gactccattc gcgacaccat caacgggaaa
    45061 attaagaagc gcgaactgtt tcgtcccttc gctccttccg tcaaagaaga agcggcatcg
    45121 aaatattttg tcctatcgca gccaagcccg ttcatgaccc tcgccgtgcc ggtcagagcc
    45181 catcaacgcg cccgcattcc cgccgtcacc cacatcgacg ggtcggccag agttcagacc
    45241 gtgaatcgag caagcaatcc gcgctactgg aatctcctgg accgcttcga aaaagaaacc
    45301 gggattccgg ttctcttgaa tacatccttc aacatccagg agccgatcgt ctgcacgccc
    45361 gaacaggcac tggcgacatt tgtacgcagc ggtgtggatg ccctggtgat cggcaattac
    45421 tttatcgatc gcgccatgct cccggcataa atctgccccc tgcggccaac gaaacccgtt
    45481 gcctctgacg cactcgacga cgtatgcccg accacccttt gcttttcttg agaagaaggc
    45541 ttacggtaac cgttactctc cgtgtcttac tccctgtcat ttgcctgcgg cctgccaccg
    45601 ccgacaacgc tcgaaaggcg gccatccgtg ctgacgtgtt cacacaaaat ctcgggctac
    45661 tcatgtgccg caactgtcct gttgccatcc cctcgaattc agccaccgtt gcatcgagtc
    45721 tttccttaga gaccaccggg caatctgagc cagacatgcg cgccaagcgg gactcagccc
    45781 tcaagccctt cacatcgaat gttgggactt gccgggcaag cagcgcaagg tagaacgcgg
    45841 agaggctagc cggcgaccac ccgacagaac caatgacacg cacacgagcg ttcgatccgg
    45901 ttattacctg gaggtgttgt atgaatggaa gaaacgttgc gcacagttca ctctgggtac
    45961 tgctcatcag cggtttcttt ggttgtgccg gcgcggggcc gtttgtcacg ctgccgagca
    46021 gcgctttttt tgagaccgac gtaaaagagc gagaggcatt gcaggtggtg agtcgcaccc
    46081 aggaagcgcg gcgggcaaat tgtcaacaga gcgccagctg tgaggaggtg acgtacctca
    46141 ggggcttagt cgcgttattc gagaaccgcg aggatgcgat ccacgccttc caagaattgc
    46201 gcaccaccgt gccaaacagt cgctatgccg cctccagcac tcgctggctc accctgctgc
    46261 aaggcggtca gccggcatcc gtcaggcacg agccgctctt catgcaactg agacaggaga
    46321 ttctccatgg cctgctggat ccggacactc ttgccgcaag tcggaaactg aaagagcagg
    46381 aacgacgaat cgccgaggta agccgctgag cgccggtatc agcaccggcc ggctgacaga
    46441 aacagaatga gcgagaaggt catgactggg ttgccccgtc gccaagaaca atcctaaccc
    46501 gcattattca tgagcacttc tgctgcaatc gccgattcac tgctgcgacg ggccttcggc
    46561 cggcgggctc gccacgtgac tcggcgattg cacgacgaac cgtcatgaat aatgcgggct
    46621 aagagctccg gatcgagcat gctttgtttg aaaacgccgg gctggttcga accgccgcat
    46681 cgcagcaaga tatcggatga cgccgcacct gattcagact ttcacgtact tcgcccggcg
    46741 ccggccttcc cggcctccac gttccctgat tcaagtccct caccagacga tcggaacgcc
    46801 tgacaggact taaggtaatc gaggatgctc gatttcgaaa tcttcaaggc ctggaaggca
    46861 tgtcgaatca aggtatgtgc cccctcgacc tcaggcagga taacttccgt cgctcccacg
    46921 tccttcaggt cctcagcctc ccgaaacccg tgcgctctgg cgagaagagg gattttttcg
    46981 ttcagccctc ggaatcgtcg aacggtaagc gacgtctcgt gaatgacggg aagcgccacg
    47041 atcgccagcg ccgcatgttg ggcaccagcc gctttcaaca gctcagtttg cgacgcgtcc
    47101 ccgtagacgc aggaaattct gcgtcggcgc agctgtcgaa tgatgtccgg gtcgcgctca
    47161 attacggtgt acggcagctc aaaatgatcg agagcgagcc ccaccgcact gcccatgcga
    47221 ccgaaaccac acagcaccac atgcccggct tggtcctcag gaaccccgcc ttgaatcggc
    47281 aaggacgctc gtcccatttt aaaatgcaat gtagcgatcc agcccggcgc ataccgaacg
    47341 aggggcgcgt tgaggagaat actcagcaac gacgcggcca gggtggcatt gtacacatcc
    47401 tcgctcacgt ggcccgcggt tttcgcgacc tgcaccaaca cgaaagaaaa ctcgccgatc
    47461 tgcgtgagac cggcgcccac gagcagggct gtgccccaag cataacgaaa cagcctcacg
    47521 accaacgtcc aaatgacgaa tttccccccg ataatcaacc cgagcatgag gccgagcaac
    47581 gggagattgg cccacaccac gcgcggatcg atgagggcac cgatcgtcac aaaaaacaga
    47641 gccacgaacg cgtcacggag cgacaggagc cgggctaagg tttcgtgacc atattctgag
    47701 ccgctgatga tcaatcctgc cagaaacgcg ccgagcgcca gggacaaccc cgccatctgt
    47761 gtgaccgctg ccgtgccgat tcctatagac aacgctacca gcagaaagag ttcggcattt
    47821 tgtgtcttcg caatgcgtgt caacaacgga ggaatggctt tcgccgcgag ataggcgaag
    47881 ggaaccagaa tgaggatcgc aagccacaag gcctttccaa tggtggcaaa acggcccggt
    47941 tccagagcac cgagcgccgg aagcaggacg gtgagcacca ccaccgccag gtcttccacg
    48001 agtgtgatcc cgatcatcac ccggccatgc ttggcacgca ggtcgccgct atccagcagc
    48061 aaccgcgcca gaaccatcgt gctcgccacg gacaccaccg acccgatcac cacgctttga
    48121 atccaactcc atcccaccac atggcccaga ccgacagcga ggagaatgga gaggaaaatg
    48181 ccgagtggcc ctcccagcag ggcgacccac cgaactcgta acaaatcatt caaggaaaat
    48241 tcgatcccga tgcagaacat caagaggatc acgccgatct cggcgaacaa ttcgaaacta
    48301 tggacatccg aaatagaggg accaggggta aaggggccga taagaatgcc gccgaagaca
    48361 tagcccagaa tcaacggttg acgaagcaga taggcggagg ccccgcctag gaccgcagcg
    48421 ataaagacat acgcgagatc tcgaaaaaaa ataggttcag gggccatgag tcctctcagg
    48481 tagaaggtcg tctgctatgc tgcagactag catgaaccaa cgctggttgc cgcgataagt
    48541 gaaatgctaa aggactgttt gataaggacg cacgcattgc atgttcggcg ggtgtcggct
    48601 actagcggtg tgtatggcga aggttccccc gttttatttt cctacagtgc agttttgcag
    48661 cttgcccgag ggctctacga ccgacgacgg cgaagaacga cggatagaga gaggtcgacc
    48721 agacaatcaa tgatctaccg ctctgcgact gtctaggata tcgaaacggg agcaacactg
    48781 ggagtgggat cggacgcatc gccgccttgt acataggttg gaaagggacg cagcgacaag
    48841 tctgaacgga accggcagta aaaccgggag tagtcggatg caaagggaat gacgaggggc
    48901 gagacctagt caggagaaat tacccagaat gccctggagg agttatccgg ccgctaagaa
    48961 ccatcgtaag ggatccgggt cgagcaagct ttgcttggag gcgccgggcg ggttcgaacc
    49021 gccgcatcgc agttttgcag cttgcccgag ggctctacga cggatggtga tcaacagcga
    49081 cagacagaga ggactaaata gtaatatcaa taacttatga cgctttcact acgccctatc
    49141 gagactgact acgactccca accgggagca aaatcgggag tggaaccggg agtaaaaccg
    49201 ggatcagggt ttgtacgttt ttgaaaagaa ttcaaaacac caattctctc tcctgatctc
    49261 agcatgtcaa actatagagt ttcatttcaa tcgcaatatt gtgcccaaac actagcaacc
    49321 ttttctaact ctaccgcaaa tgtcacgaat ttctaggccc cttcctgcat gccactacgc
    49381 cggctagaat cgatctaggt tacgggccac attaccggct cccattcaat ggcaatctat
    49441 attggcaccg ttctgaaaac aatgaacagg ccgatttggg ttcgttggta taggatgtgc
    49501 cacagatttt ggtcctccta gacttgtcca accgattgct accaaagccc catttttgaa
    49561 cacgtagggc gtgaattcgt catccgttgt caagccatca gcctgccggc tactccgaaa
    49621 aaagaatatt tctgtgagca aattgtcggt cacaaaagat tctggacgct ttgatgcctg
    49681 tttcgataaa cccgcttgag tgggtataag gattgccaaa accttctctt tggaatcacc
    49741 taatttgatg ccggctgatt tctcattgta ctcagatatt gcattatcga ttgaacgtaa
    49801 ttctctcggc atgcagctag tgcacagcag tagaggcatt gctaccagca aaatggtagt
    49861 agttttataa agagccattt tatcccccgc atagccgtgc aacttatcca caattggtta
    49921 gaatatcgcc ttaagaacca ccataatagc cgccattcca ataaccactg gaagtatcac
    49981 atctaagacc aaagccttta actgggttag atttaagtac caggaccgac gcatagaaaa
    50041 actcttccat tctttctcat agtaatccgg tgggtctttc cagtcagggg taggctcatc
    50101 ataatctgat ccatactttt cttcgtattc ccatctatgc ctagctccac gaaggtcatg
    50161 ttcagcaatt tgaactttcc agcgcaaaaa atctggcagg gcgtgcaaaa caaatgagag
    50221 taaaaaatac gagacgacca tgataataag tctgaggagt tcgatctcat taatttcagt
    50281 gacctcgata gtcagaaagc ttatctgcga tgggactatg ccggccttcg ccatggcaaa
    50341 ggctaccgcg gaggagatga gcaacgcctt acgttctttt cgtgcaaggt tgcctaaagg
    50401 gtcggcttcc aggatctgct caatattttc tgccatggat agacttcaac gctagttgcg
    50461 aagatcacat acacagtatc ccgcgccaac cgattttagc cgcacacgaa tatacctaca
    50521 tgcgtacgcg aaatcgagct tgacaaaggg tgcatacctc gatttcttgg agtacgagta
    50581 aatgaaagtt gaacccaggc ggtgcctatc gatggcccca cagtatccaa ttgagccgtg
    50641 ctgggaagtc ctactttgac catatagatc aagtgaagcg ggggctgcgc ccccgatccc
    50701 cccggccgtc cggtccagtg gtccggaacg gccgggcggc ctcgttgggc tgagtctttg
    50761 cgttcctaag ctccccatgg tttcagtatc cctgttcttc tcttactctt tgtttataag
    50821 caacgatcca acaggacggc ggccaagctg acgtatcgcc gccttgctca aggatggagc
    50881 atgttccctc tcacccgtgg cggatagtgg acgggtgaca gggagaaatt gattagaaca
    50941 tttcactgtc tcgcagtgag ccccgcacaa tccacacaga gccagatttc gcacgtcacg
    51001 tcgttctccg taaaacagag ccgaacccag gctcgttcgt gttggcagaa ctcacacaat
    51061 cgttccttgg tgtgttcagg acagagccaa gcttttcgtg cccggtcgta ggttgtcgct
    51121 tcccgtccgc aacaggccgg tccaaataga ctcggcagct taagatcgca tcgatgccag
    51181 atcatcctca cacctcctgg taaacgaggc ctttccccag cacgagttgc agaatggtgc
    51241 cactgaacca aatggggtgc cctgagagca gatcctccca ggtcttcaga aagacgatgc
    51301 gcgggatttc gatttctccc ctgatccgcc gatacacgtt ccggctcacc cattggttcc
    51361 gcatctcttc ccagagcctg ctgatgggaa acccaagaga tcgcttccag gaccaacaca
    51421 tattcacgta cccgcattga tcctgaacgt attggctgat gacgtatcgg ctgaggcggt
    51481 ttcgagaacg atgactgggc tggtaggcct tgatccagac caccggagcc ccgtggaggg
    51541 cctgccactg tgacgaaagc cattcttgcg agatccagaa ccggcgcgcg cgttccccgt
    51601 ccggcacccg ccaggcccag aagatatgga ggacgccatg tccctcttcc gtccgcacct
    51661 ggtagtactc caatccctga aaccccagct ggcgttcgat ccgttggcgc agttgtttgt
    51721 gatggtacgt cagcttttcc gcatccccgc cttctgccgt ggagagtgtc acccagagga
    51781 cttggaattg gtggcactcc cagaaccaca gcagcgaccg gacccggtga tagccccgct
    51841 tctgcttcct ggtccattcc ccggcgattg gctcccgccg gaactccttc cagcggctca
    51901 tcgggcaccc tccgcctgag gaatcgccgg tctcagaccg accgcaagaa tccgctccag
    51961 ttcctcgata ggaatccgca cagctcgcac ggacggtttc acataggcaa tctgccgttt
    52021 caggatgtat ttcctgatcg tgctttcttt gagtcctaac cgctccgccg cttctcgaat
    52081 ggtgatcaat tttgtactga ccatctggca cctccttggc aatggttata tatttggtta
    52141 tgcctcattc cagtaaggca tatcgtctat tgacacgcat taccactacc gaggtacact
    52201 cttcaagcga tttcagctct acgcctaccg ccggtcgtaa tttttttgtc tgtactagga
    52261 tggagtgaga ttctcatcca ctcgtcagga gaaggagtac cctatgccga tagatcgagt
    52321 cgcaatccac gaccaatttc acggagagat gaagaaggcg agagagagga ttctcaccac
    52381 cgcagacggc gaggtgggat ggctcttgaa gtttattcag actgatctgg acaccctcac
    52441 ggccagtgaa tggatggtct tggcgtttga gatcgccagc tttgtcgatg acgttgccaa
    52501 tcgccggggg gcggaaatcg ccacagaggc tggatggagc gttcgatcga ttccagggga
    52561 cggatttcgc ggcacccttc ccagtcgagg ggaggcgaag gagatccagg cgatggtcct
    52621 gggaagcctg gaaaagctat gggaaaccgc cgtcgccgcc tttacgttcc cgcaattcac
    52681 gatcattgtc acgttgccag tcgaagacga acgcaaaggc agtgtgtttg tggccacaaa
    52741 gcgaaaagtg aaggaatttg aatatcggtt tgcccatctg ttagtggact atgccgggcg
    52801 cattcgccgt tgcccagagt gccaacgtat ttacctcgcc attcgtttcg atcaaagata
    52861 ctgtggccct cgttgtcaga cgcgggtcgc gactcgcaaa tggcgagaaa cacatctacc
    52921 taagaaacca aaagcactgg cagatacgac gcgcaccaag gctgatccaa ccagaaaacg
    52981 ccgaaacgga ggctccgatg gcaagacgaa acggtaaaga ccgtggagtc gtatttaagc
    53041 aaggcggttg gtgggttcgc ctgtatgtga atggccggga gaagtggttc cgctcagact
    53101 ccaaaagcca agccaagacg ctctacggtc ggttgcggtc cgaagctcgc gagaaccgct
    53161 actttcccga gaagtatgcc gtctctaaag atttgaccct caggacttgg attgcgcgat
    53221 atctggaagg ggtgacctcc ccaggtcttc ggaatgtgca tcgctatgga aagttctggt
    53281 cgaagctcct ggggcggaag cttttaaccg aaatcaccgc cgacgactta cggcatatcc
    53341 aagccaaaat gaagcacaag ggcacccgca ccgctcgaac gattaaccgg tactttggag
    53401 ccctgcgacg cattctaaac ttggcgattg ccgatggtct gctttcggcc aatccagtca
    53461 agggtgtgaa gttcttttcc gagccagccg ggaggcttcg cttcctgagt gataaggaaa
    53521 tctccagcct gcgagatacc ctgcctccgg agcactggtt gattgtggcc tttgcgctcg
    53581 aaacaggcct acgcctcacc gagcagtttc attcccgttg ggactgcctg aataccgacc
    53641 aaggcatcct aaccattcca ctgagtaaat ccggcaagac tcgccacgtc attctgacgg
    53701 atgccgctct ggacatcgtc aagggtctca cctcctggat gcacagcccc tttctttttc
    53761 cgagtcctct cacctccgca caaccaatgc agggacgaaa ctttgtagtg aagatttacg
    53821 aaccagcctt gaagcgagca aaaatcgaag gagttacctg gcacaccttg cggcatacct
    53881 tcgcgtctcg ggctgtcatg gcaggagtcg atatccggac cgttcaagaa ttgatgggcc
    53941 attccaccat cacaatgacg atgcgatacg cccatctctc gccggctcac ctgcgaacgg
    54001 cagtgaatcg agcgagtttg ggagccattg cgtcaaaaag tgagagtgga accgggagta
    54061 aaaccgggag taacgaaaat cagggggtgg aacagggtcc ggtcagaatc accgaaccct
    54121 ctgagatatt tacaggaatg gttggaggcg ccgggcgggt tcgaaccgcc gcatcgcagt
    54181 tttgcagact gctcccttag ccacttgggt acggcgccac ggatcggatt atagaagggg
    54241 gagcttcagg aactcaaccg cgaagagcag agaaatggag gaatgttcaa aacatagtcc
    54301 aactgagggg gatccgcttc tgatcagcgt ttcgtcggca aaacaggaat atcgcgaacg
    54361 atggtggaat gctcatccgg cgcatgaccg atcaattccc acccaggctc cgcctgccca
    54421 tctgacgatg ctgccgtttc gtgagtccct ccagcggcct cggcttcttt ttccttggcc
    54481 agcaactcga cctcgtggat cagcgtgtcc atgagttctt caagcggcac tttccgcatc
    54541 aatttgccct tcttgaagag aatccctttg ccttcaccgc cggcgatgcc gatatccgct
    54601 tccttgcctt ccccgatgcc gttcacgaca cagccgagca cggagacgtt cagcggcgtc
    54661 ttgatatggc cgagcttctt ttccagttca ttcgccatgc gcaccacatc gatctcaacc
    54721 cggccgcagg tcggacaggc aatcacattg ataccacgat ggcgcaactc cagcgacttg
    54781 agaatctcga acccgacttt gacttcctcc accggatcag ccgccaacga gactcgaagg
    54841 gtatccccga tgccctgcga gagcagccaa ccgaggccga ttgcggactt gaccgcaccc
    54901 gtcatcgccg tgcccgcttc cgtgattccg atatgtagcg gataattcga ctgggtggag
    54961 aacaaataat aggcatcgat ggcatggtgc acgtcggaag ccttgagcga caccttcata
    55021 ttggtgaagc cctcgtcttc caaggcatgg acggcattta aagccgactc agacagggct
    55081 tcgggagaag gccaaccata cttgtccaac agcggacgtt cgagcgagcc gccgttcacg
    55141 cccacgcgca ggggaatgcc ccgctcattg acggccttaa tgacttcctg caccttccac
    55201 caagcgccga tgttgcctgg attgatgcgg acgcaatcca ccacttccgc cgccttgagg
    55261 gctaagcgat ggtcaaagtg aatgtcggcg atcagcggaa ccgtcatcgc ggctttgatc
    55321 tgaggaagcg cagctgccgc cgcctcatca gggacggcga cacgaatgat ttcacacccg
    55381 gcctcttcca gctgacggat ctgagccacc gtggcgttca catcgcgtgt atccgtcgag
    55441 cacatcgact ggactgaaat cggcgcatct ccgccgatct tgaccgatcc cgcctgaatc
    55501 tgccgcgtct tttttctcgt gatatgcatc agcttctcag cgtatgtcct gttttggtgc
    55561 gctccgacgt caactaactg ccctgctcat cgccgtgcgg caaactcccc accagcgacg
    55621 ccaaccgggc ggcatcatcc gtggccacac tggccccgaa caaggccttc acgctgttgt
    55681 aaattccgtc tgccgtcagg ccataccgtt cgcgaagcag gtcttgcgga ccctgctcga
    55741 tgtaccaatc gggcaggccg atcatcttcg ttctcaagtt cgtgatacct tcttcggata
    55801 aagcttccaa gaccgcggaa ccgaatccac ccatcttgca gccttcttcg acagtcacca
    55861 ggcagcgcac gttcttcgcc gtcttgatga tcaattccgt atccaggggc ttcacgaaac
    55921 gggcgttgac caccgcagcg gagatcccct cctgcgccaa ccgttcagcc gccttcatgg
    55981 cgggccagac ggtgactccg atcgccacaa tcgccacatc cgtgccttcc cgcaacaact
    56041 cgcccttgcc gatcggcaac gccgtcggtt ctggatccat cggcaccccg agactcacgc
    56101 cgcgcgcgta ccgcaccgag gcgggcccgt catgcgtcac acaggtcttc atcatgtgtt
    56161 gcagctcgtt ttcgtccttc ggtgctgcca ccaccatatt cggcacatgg cgcaggaagg
    56221 caaaatcgaa cgcgccgtgg tgcgtcgtac catcctcggc caccagaccg ccccggtcga
    56281 tgcagaaggt caccggcagg ttctgagtag ccacatcgtg caccacctgg tcataggccc
    56341 gctgcaagaa tgtggaatag agcgcgacca ccggcttcat gccctgcgca gccataccgg
    56401 cggcgaaggt gacagcatgt tgctcggcga tacccacgtc gtaaatccga tccgggaaca
    56461 ccttctcgaa cgcattgagg cccgtgccct cgcacatggc ggcggtaaca gccacgatcc
    56521 gcttgtcctg atgagcgact ttgatcagcg catcaatcgc catactcgta tagctggggc
    56581 gcgatgcttt cttggccggc acacccgttt cacgcacaaa gggcggacag gcatggaacc
    56641 agaccggatt atccatggcc gcctgatagc ccaagccctt cttcgtaatc acgtgcagca
    56701 ggaccgggcc cttcattttc agcacgttct ccaaggtcgg cagcaggtgc tcgaaattat
    56761 gtccatcgat cggtccggca tactggaagc ccaattcctc aaacaacaga ccggggagaa
    56821 tcgccccctt ggccaattcc tcagcccgtc gggcgatttt ctgcacctca aggccgatgt
    56881 ggggaatctt ccgcaacagt tggccggttt cttcccgcat acgcgcgtag aattcgccgg
    56941 tgaaggtccg gttcaggtag gccgaaatcg ccccgacatt cttcgagatg gacatctggt
    57001 tgtcgttcag gacgacgatg aaatccttgt tggtgccgcc ggcatggtgc aacccttcga
    57061 gggtcatccc ggccgtcatg gcgccgtcac ccaccacgca gacaactttg tgtttttcgt
    57121 tccgctgctc gcgcgcctcc accatgccga atgcggcgga gactccggtg ccggcatgcc
    57181 cggcattgaa ggtatcgtag acactctcct cgcgcttaca gaacccgctc aacccgccgt
    57241 actgccggag cgtatggaac tgctcccggc gtccggtcag caatttgtgg gtataggcct
    57301 gattgctggt atcccacaca atcttgtctt tcggcgtatc caggagatat tgcagcgcga
    57361 ccgtcagctc caccacaccc agattggagg ccaggtgccc cccgacattc gaaaccaccg
    57421 taaggatctg ctcacggatc tcctggcaca gttccggaaa ctgctcaggg gagagacgtt
    57481 tcaaatcagt gggactgtgg atatttttca gcagagacat tgcacaatct ccttgtcaaa
    57541 tgatgacaat tggacgcagg tattcattca gtgggaaaca atcgtattga gaaggtcgcg
    57601 acgaagcagc gaagaagttc gtacaatcgg cttatcctca catatggcac tgcctgtgtc
    57661 aagatttcgc cttcgcccgc gccaaaaact tcgccctcgc gactacgacc gaaatcggta
    57721 ggtgaggacg gtggagagcg taaaatcggc cgcgctcccg gtaatgaaat caagattctc
    57781 cactccatag agctgccaga gcagaccggg aagaattcga taactcaccc ccagcaccga
    57841 ctccgtcacg cccttatcca ggactcgcgt gcctgtcccg tgaaacggcg gggaataata
    57901 atcgaactgt accgtgatgg aaaagttctc cgtccagaga tattccacgg caaccaaccc
    57961 gctcaccacc ggctggagtc ccaacccggc aatccgcccc gtaggaaata ccccgttgag
    58021 attcgcgtac aggatccagt tacccgcgaa cttcttatcc agcgccaggc cgataccggc
    58081 gtcaggatgc ccgctgccga acacttcgcc ggtgtctccg gtcggagcct tgaccccgac
    58141 ccggactgac agggctggaa ggctcgaggt ttctttgaga atctggtact tgccgtagaa
    58201 cgagatgtct cccaatccag tcgcgccttc ggatccctga aacagcgtgc gggagccgtt
    58261 tgaaatattg aaggcgtacc cggtctcccg cagggccttg cgcggcggcg ccacgccggt
    58321 cgtgccccgt tccaccccga tgatcgcccc ctccatgaag ccgcggtacc gatgatagcc
    58381 gggaatctct gcgccgagct ccaatcgatc ggtgagacca taccggagga acaggccggc
    58441 ccggatcgtt tcaaacttca tcgccacctc ggcctgcgct tcactgtccc tggcaatgct
    58501 ggccgtattc gccgcttcca gccgcacatc gaaatcgccc ttgcgaagca ccgtcgcccg
    58561 ctcgcccggc atggccagga ccaattgatc cagcgcttga aagtttctga ctggaaacgg
    58621 accgaagcct tccgctccgg cagcaggcgg aaggacactc agaagtgcaa ggaggcagaa
    58681 acaatgaatg gtgtaccgca agagtaaccc cggccgttca ctcagtggca cttaataggg
    58741 aaagtctaac cctgcgaaga ccgctccgcc ctcgcgactg tacccgtaat cgacccggcc
    58801 gacgacattg ggacgaacga tggcgcgaaa ccccaacccc ggcgtcattc gataatcttg
    58861 aaagctgacg tctttgaatg agttgaagac ctgccccgtg tcgagaaacg gcgccacctc
    58921 gaaatccgca gtcaccccgg ccagtttcgt cctcaagatg tgaattcgct cttcgatact
    58981 aaaggcaatc agatgtttgt cgatgtaccg gtccaagccg aacccgcgca gattgttctg
    59041 ccccccgagc gaagactgtt cgaaaaacgg cacctgggat ccgatcgtcg cctgcaggtc
    59101 ggcgcgaacc accagaatgg cgcgcttgga ctcgctgggg aataattttt tgacttcgag
    59161 ttcataccgc gaatagaccg ggtggtcgcc gtttttcaca ttttgattca gttcggcata
    59221 ggcggtgatg gccacgccgt cggtcgggga aaccaagctg tcacgggtat cgtaataaaa
    59281 cgaggcgcgg tggcccacga tgatggactc gccttgaata ccgtcaaccg tgggaaattg
    59341 ttcgaccgaa aagggcagat cgatcgctcc tcgctggagc tgcacctgcc gaacccgctg
    59401 cccgacagaa atctgtgtca cttcattcgc gtagaggccg agccgccagt aggcgcgcgc
    59461 ttccctggcc gtatagttgg actcggcggc ctgggtcgtc gcctggccga ggccgaagaa
    59521 ccgtgaggtc gcgttcttaa aaaaggtgcc gccgaaattg agtgaatatt gcccgttgcc
    59581 gaatgccgga tcgacataat cgaacaacac cttgcgttcg atccgttcgg tcaacgaggc
    59641 gatgaatcgc atctgtcgtc cacccggatc gtacttaaac agattgaaga ccccgcgtgt
    59701 acccacgatc gagttctgaa tgagcatggg cgccatcaga tacttcagtt ctccgtccgg
    59761 gtccgcaatc aggatcggcg cgatcaaacc ggcgtcgttg ccgtcgttcc gactggtcga
    59821 gacggaggga accgggaaaa tctgcgtgtc ggcgccggcc ggagccggaa ggctcaggag
    59881 cagaaggccg gccagcgcca gacagcttcg aatcgacgac aaatagtgac actgcatggc
    59941 gctcgccgga atcgctcgct tcctaccgga gctacggagc tttgatcttg tcggatttct
    60001 tgcggagttg atcgacgagt tcgggatacg aggaggtgtg gaggatcttt gtaaattgtc
    60061 cgcggtagtt gttcaccaga ctgacgccat cgaccacgac atcgtacaca tgccaatcgt
    60121 tgctcttgtt cagcaaccgg taatccatgg ggatttcagt tttccccgat agcaccttgg
    60181 tgcggacttc cgcatattct ttctcggtcc gctcattcag atactgcaca ccctcacctg
    60241 aataggtctc cacacgatca gcgtacgtat tggtcaagag cgtccggaac agatcgatga
    60301 actcttgctt atccttgtcg gacaactgat tccactgtgc acccaacgac cgccgggaca
    60361 tttctggata gtcgaaccgc tgggccacca ctttttccaa gcgctgccgc cggtcctcca
    60421 ggcgggccgg ggctttcaat tctttatcgt tcaagatcct gagcacctcg tcgatggtgc
    60481 ccttaacaga atcagtcgcc ggtccggcca tggcggacga aagcccgccc accactatct
    60541 gcagggccag caaggccccg atcatcatcc aacggcttgt atgcattcca acacctctcc
    60601 tttgagtggt gagtccctga ccccaccggc ctggcttgtt cactttcatc tcctgacagc
    60661 tgacacgatc agcctagttg accttcccgt gtacatactg gctgaccaat tcttcgagat
    60721 caagtccgga ctcggtatca cgaatcttgt cacctggctt taacggatcg ccgccgccgc
    60781 ccaccgagag ggcaagatac ttatccccga taatcccgcg agtcttgatg gaggcgatcg
    60841 tatcggaata caacttcacg ccgtcttgaa tggccaatgc caccagggca cggtcgctgc
    60901 tcagaccgat gttcctgacg cggcccacct cgacaccggc gatttcgacc gacgcgcccg
    60961 gcttgagtcc cgaggcggag gtaaactccg cctcaaccgg gtagttgtgc cctccgatga
    61021 cctccaactt gcccaatttg atggacaggt agcctagaca ggcgattccg acgagcacaa
    61081 agattcccac catcaattcc agctttgcgc gttccataac gtctcctatc agagggatgc
    61141 ggtcggcaac cccttcggcg tgacggtccc ggcgacaaaa ataaactccc gaatctcttc
    61201 atccgtcgtc ttcacaaact cgttcgatgg cgccatttcg gcgatccgtc cgttcttgag
    61261 catcgccaca tagtcggaaa tcccgaaaat ctcggggatc tcgtgactga ccatgacagc
    61321 agtaaacttg aattgttgct gcatcgccac gatcagatca tgaatggctt tcgccatcaa
    61381 cggatcgagc cccgtcgtcg gttcgtcgaa cagaatgatc tccggctcca tgaccagggc
    61441 acgcgccaac ccggcgcgtt tgcgcatgcc tccgctcagt tcggcgggaa acttatggcc
    61501 catgcccttc agtcccacct gttccagttt ctcctcaact ctccgggtga cgatctcgcc
    61561 cttcaggcga agcttttctc gtagggggaa ggcgacattt tcaaataccg tcatcgagtc
    61621 aaacaaggcc gcaccctgga acaacatggc gaacttcttg cgcacctcat tgagcgcctg
    61681 ccccttgaga cgcgcaatgt tcgtcccgtc gatccagacc tcgccgcgat ccggctgcat
    61741 aaggccgatg atatgcttca gtagcacgct cttgccttcg ccgcttcgcc cgatgatcgt
    61801 cgtcaacttt ccagtgggaa tggtcaggtc cacgccccgc aacaccggct gcttgcccaa
    61861 agtcttttcg acgccgacca gtttaatcat ggagcctcgt acgggcggca gctgacccgt
    61921 tccgccgtag ccgcattctc ctcatgcgct tcacgtttca cgatgttaga gcagcaccga
    61981 cgtcaaaaaa taatcccaca ccagaatcaa gaccgccgag agcaccactg cttcggtcgt
    62041 cgcggtcccc aacccttcgg cactatgcct ggtgtgaaag cctttgtaac aacagaccca
    62101 actgatcagc aggccgaagc tgatggattt caaaatgcct ccatagacat ccttccactc
    62161 gacggccgac tcaatcgaac tccagtaggc gccttcattc ccgttcaaca gttgcacgcc
    62221 gacgacgtat cccccataaa tcccgacgac gtcaaagagc gcgaccagca gcggcacggc
    62281 gatgagactg gcgacgagtt taggcccgat cagatattgc agtggattga tggccatcgt
    62341 gtccagagca tcgatctgtt ccgtaatccg catgatgccg atttccgcgg tcatggcgga
    62401 tccggcccgg gccgtcacca tcaacgccgc cagaacggga cccaattccc ggatgatgct
    62461 gagcgccacg gcagagccga gcaccgcttc agaaccgaat tttcggagcg tgtaatgccc
    62521 ctgcagggcc aggaccatgc cggtaaaaac ggcagtgagg acgacgacaa acgtggactt
    62581 gtagccgatg aaattcagct gcttgacgat ttggtaaacg cggaacggcg gacgcgtcag
    62641 ccaggcaaag gaggagagga cgaacaacat catccgtccc atctgctctg tcagatcgat
    62701 cgtataccgt ccgatacgag caatgccttc catgccacgc tctcctctcg tccacctacc
    62761 agaaactcag ccgtcgaaac acgttgggcc cggtcaggcc agtccctcgc gtaacgcctt
    62821 atccgcgcag gctctgaata ccgccgtcaa ttgtgtaccg gcgatgcggc tttgcacacg
    62881 aagtcgattg agtccgatga gactcgtcgg atgagccaga caagcggcga cacccttcac
    62941 ccatccagat ggcctgagaa acagattgaa gtcaagcgga aggctttcat cgacaagatc
    63001 cgacgccgta cgaacaatga caaatggaat cttgcgctcc gaagccacaa accccaacgc
    63061 cgcactctcc atgtcgaggc cgacccctcc cgctcgagtg gcaatctcct gcttgtcctc
    63121 cgcccggcag agaacccgcg gcaccgaaac gaactgcccg gcctgcaccg ggaggcgctg
    63181 ttgctgcacg gcctgccgca cccaatcggc tccggccgca gcacagggaa cggaccgccc
    63241 gtcctccgcg ccaccttcca tggtcacgtg agttccgatc aacacctcgc cgataacggc
    63301 ctgacccaac gcacaggcaa accccgtgga ccacaccgcg gcaaatgact gttccccgag
    63361 cacccgtccg gcggtcgtgg tggcccgctc gggtccgacc cccatcggga tgacccacca
    63421 ctcgacctga ccctgccgcg caacggcaca acgaatccct ccggcgaccc gcatctcgct
    63481 cgcggcaaac gcctgacgca cggcggccga ctcccaccgg gttgccacga agactccgat
    63541 cgcggtcaca atgactcccg agggacaatg gcaggaaaag ctgaactcga tgaacaccca
    63601 cggtcagcgt ggcgaccgga attgtcctga ttgataggcc tgcagacgca attcatcggc
    63661 cctcgtcgtt ccacgagtct tgagattgcg gtacatggcc agcgcccaga gggggaagta
    63721 ttggcaatac caatgataac gaaggtaaaa cacgcgcggg aaaccagttc cggtatggcg
    63781 cacctcttcc catgacccat ccttccgctg attccgaatg aggtaatgga tccccctggc
    63841 caggctgaag gagtccgtca ctccgcctgc catcaaggcc aacagcgccc aggcagtttg
    63901 cgacggtgtg ctgtcaccgc gaccgctctg cgagacatct ccatacgaga ggcaggattc
    63961 accccatccg ccatcggggt tttgcttgga ctcgacccaa ctgaccgccc ggcggatata
    64021 gggagacgag agatcctcac cgatcgctcg caggccggac aggaccgacc aggtgccata
    64081 aatgtagttc acgccccaac ggccatacca actgccgtcc tgctcctgat cgtttttcac
    64141 aaacgtgagc gcagaagcca cggcaggatg ggtccagtcg tatcccagcg tcgcaagcat
    64201 ctccagacag cgtccggcaa ggtctgccgt gctgggatcg agcagggccc gatggtcggc
    64261 gaacggaatg tagttgaaaa caatccgatt gttatcgacg tcgtaggcgc cccagcctcc
    64321 gtccgatccc tgcatagagg tcacccagcg gcaaccgcga cgaatcgcca gacgttgctg
    64381 ggcttcatcg gacagatgca ccttcgccag ggccatcagc acgaccgcgg aatcgtccac
    64441 atcgggaaac agttcgttct cgaattggaa ataccatccg cccggttcgg caccgggagc
    64501 cgaaataatc caatcgccga cggtcttagt ctgcttggac aataaccagg tcgaagcctt
    64561 ctgcagcgcc ggatgatctt gcggcatacc ggcctcgatc aaggcattga tgagcaaggc
    64621 cgtgtcccag atcggggagt ggcagggctg caaatgcatc gcatcgacgc attggccgtt
    64681 gtcctgcacc gtgtcgtgta cttccagttc ttcgatctcc cggatggcct tcaccataag
    64741 gggatcatcg tttttgtagc cgaggcaatg cagcgccatc acggaattgg ccatggcagg
    64801 ataaatcgcg ccgaggcctc cgctgccctt catgtggtcg agcatccagt tctcagcgca
    64861 cttgagcgcc ttctgtcgaa cccactcaac gggcgaacga tcgtagatct tgagcagcgc
    64921 atcgagattg atgaagaagt tcctggccgt gaaccaggtc cggtccttct tgaacggagg
    64981 aaccatgccg taatcgatct ctgccggggg ctccgtatag agttcgtcga tgcccagttc
    65041 cggcggaaca tgacacaagg gccgtttggc gaatatgatc aacagcggaa tcagcaccgc
    65101 acgagaccag taggaaatcg cgtaaatgct gaaatagaat cgcttcggca acagcatgat
    65161 ttccggaggc atgctgggga caccacgcca gtcatattga ccgaacaggg ccagggcgat
    65221 tttcgtgaag acatttgccg ccaccactcc acccttgtcc aggatgcagg tgcgggcatt
    65281 caccatgaag ggctcgtcgg cggacacgcc ggagagcttc aacgcaaagt aggccttcac
    65341 cgaagcgctg atctccgccg ggccaccgtg ataaatcgac catccgccgt ctggcaactg
    65401 agcggatttt aagtagcgga ccgctttgcg ctcgcgctcg ggatcgatgc agtccaggaa
    65461 gcggcgcaac atcagatact cagatgtcag cgtggtatcg gcttccaact cggcaaccca
    65521 gtacccttcg gacgcgtctt gtcgcgccag aaaccaggcc tggcttttcc gcagcgcctc
    65581 ttccagcgct tccacctggc ctgtggaatg tgccggggct cgcttcgcgg cactgtcgcc
    65641 ggaagccggc accgcagact tattggagac gagtcggagc accggagcgg aggcaaagtc
    65701 gcgcgccgcc ctgattcggc tcggcaccga caccagcaaa ctatcggaca aacgattgaa
    65761 caggtttttg agaagtcgca taacgtgatg ccgtgaatct gaaaaagacg gcgggattga
    65821 tagtcgagac ggttccatcg atcaggaagc gatcacatga aaccgcacag taccacttat
    65881 aatcattagc agaactgtat aacaaactgt ttctaggcag tcaagggaag cagcccccct
    65941 cgcgcggcgg cattgccttc accacccgcc ggaacagccc ctacgtactc atctccgctc
    66001 ggactacgcc ggctggagat caccgagctt gactgaaatc aacgtcgaca caccgggctc
    66061 ttccatcgtc accccgaaca gcgaatccgc catcgccatc gtccgcttat tgtgcgtgat
    66121 gaccatgaac tgagcggtcg acgacaagct gcgcaacacg ctcgtgaaac gcccgatatt
    66181 ttcttcgtcg agcggcgcat cgatttcgtc caggatgcag aacggcgtcg ggcggatcag
    66241 gaagctggcg atgagcagcg ccatcgcggt cagcgtcttc tctccgccgg acaacatggt
    66301 aatgctcttc aagcgcttcc ccggcggctg ggccacaatc tccacaccgg gctcgcgcgc
    66361 cccgttgtct tccacgccct cttccaacgg ctcttcgacc agttgcaatt cagcgcgacc
    66421 tccggggaag aactgggaaa agacgtcccg gaatttctgc tgcaactcgt tgaacgtctc
    66481 cacgaacatg tccttcgtcg tccggttaat ccgctggatg atctccttga gcgacgcgat
    66541 ggaggtcgag aggtcctgct cctgcgtcgt gaggaactga taccgctctt ccaactcacg
    66601 atgctcgtcg atggccgcca gattgatggc gcccatccgg tccaggcgct cacggatttt
    66661 ctgaatctgc tcccgcaatt gcggcgtttc aagaatcgag acgggggccg gcggttcttc
    66721 accctccggc accggctgat cactccccgg caggagcagc gtagccggct cgatctgata
    66781 ggtgccggcg agcgtgctct caatcgtgga gagatgcgcc ttcacttctg cctttcgcac
    66841 ttcagccctc atccgtcgat catgcaaggc ggagaggctc tgccgtacct ggctaagagt
    66901 ctcctccacc gcgtgcaacc cggccatttc ctgtgcctgc cgctcctggg ccgccactaa
    66961 ctccgccttg attccgtcta cctcagcgcc caattcccga cacaatgctt cctgcctggt
    67021 ttgctcttcg cgactattct cagtcgcttc aaccaacccg gccacctgct cttccaaatc
    67081 ggtcgcgcgg cgttgcgcgg catcgagtcg ttgcgtaaac cgtgcaatgt cgttcgtggc
    67141 atgttcccgg cgcccgcgca tgctctccag agacaaccgg acttcggtca accgttgctg
    67201 gatcgccaga ctttggcttt cgatcaccac caaccgttct cgcacgtgca gcaacccggt
    67261 ttcctgcccg accttttccg ccacccattg ccccaactgc gtctggctgg actgaaactc
    67321 ttcctgaagc cgcgcctgct cggcaaagcc ccgctgcagc tcctccgcca acacctccat
    67381 ccgccggtgc aattccccgc gctggcgttc cacgcccgca tcgtctttct gcagcgacaa
    67441 ctccagcatc tctgcttcgc ggatcgcccg gctgaggcgc tgctcatctt cacgtccgag
    67501 ccccaactcg gccgccgcgt cctcccgcgc cgaccgtgcc tgctcaaggg cttgaaccgc
    67561 tgcagtcctc cgagcttcca gctccaatac ttcgcgccga cgttggagca agcccccact
    67621 ggcactgctg ccgcccccgg tcatcacacc ggcggcgtcc agcgtttcac cggataacgt
    67681 gacatatgtt ggcccggcgg gagcagccca ttgatgctgc tgccaaagct gcagcgccac
    67741 gtccagcgac tccacaaaca caatcccgtc gaacaaatag ctcaaggtag cggcagaggc
    67801 accttccgcg cggatcaaat ccgtcgcgcg ccccaccacg cccgcctgcc cggtcagcgt
    67861 cggccaccac gaggccgatg aatcaagcgc cgcctgctcg ggagcccatc gcaattgctg
    67921 cggaagaaac gctccgcgcc ccagttcttt gcccttcaaa aactccaccg cacgacaggc
    67981 cgccgatggc tcatccacca gccagccgcg cacacgttcc ccgaggatcg tctctacggc
    68041 ccggtccagc cccggcggaa tcacgagcca ctcggcgagc gcttcgcgca cgccgtcgca
    68101 cgtcttcaac gccgtgcctt cctcctcacc ttcccggcca tatcccattt cctcacgcac
    68161 gacaccctgc agggcgccga ggcgcgattc cacgccggcg acgtcttcgg aaaactttac
    68221 gatatcccgg tctagcccgg tgatctgccc gcccagccct tcgatccgct cgatcgccgc
    68281 ctgccgttcg gccatgagtt cttgaatgcg cccgctggcc tcttcgcgtt gcgcggccag
    68341 caagtcgcgc tgctgatcca acccggccac ctgtgccacc aactgttcct gttcgcgcga
    68401 gacccgttcc gcccgggcgg cggtttcctg ctgacgggcc gtcacctgcg tcaggctctg
    68461 ctctgtattg gcaacgagca cggccaggtt caacacatct ttccgcgcgc gctcttcctc
    68521 cgccaccgcg gaagcgcgat gcccggacaa cgatttcgcc tcgacatccg cctgctggaa
    68581 ttgcgcttcc tgttcgacga tgtccccttc caattgaagc agcatcgccc gaagggtctc
    68641 gatttccgac tgcgcctgct cctgatccgc cgccaaccgt tccatttctt gcgcggcctg
    68701 catgcgttga cgttcaaaca attccgtgcg gttccgttcg acctccgcgg ccgtcaaggc
    68761 ctgcgactgc cgctgttcgg tgccggccaa tgtgtcacgt acacgcgaaa tggcctccgc
    68821 cgcgtcgttc atgcgcagcc tgatcgcctc ctgctcagag tcgaggcggg cttgttcggc
    68881 cacctgttcc gcttcctgcg cttccacctc gcgcgcttcg tgatcgacgg actccaggtc
    68941 ggcatgcatg gtgcgatagt cgcgggacag gagttcgatt tcgaggccgc gagcctcctg
    69001 ctgcaacgtt tgatacgaac gcgcctggcg agcctgacgc tccagcgaat tcagctgttt
    69061 tttgacctcg gccacgatgt cgcgcacccg caccagattc tgctgcgtcg cctcaagctt
    69121 gcgaagggct tcggcctttt gttttttgta gcggacgata ccggccgttt cctcgatcag
    69181 ctcccggcga tcctgcggcg aggcctgcag gatctgctca atccgcccct gctcgatgac
    69241 ggtatggccc ttggacccgg ccctggtttc aatcagcacg tttcgaatgt ctttgagccg
    69301 gcagggtgtc ttattgatga gatattcgct gtctccgttt cgatacagac ggcgggtgat
    69361 catcagttcc tgatattcgc tcagttcgct cggcagcccg gagatcgcat ccagccgtaa
    69421 ttctcccagc ccgccgatga ccagcgacac ctccaccagt cccaacggct tgcgaacctc
    69481 ggtgccgttg aagatgacgt cttccatctt ctcgcttcga agcgtcttcg tactttgctc
    69541 acccagcacc cagaggatgg agtccacgac attacttttg ccgctcccgt tcgggcccac
    69601 gatggccgtg atgcctttcg gaaattggat cttcgcctcc gcgaacgact tgaagccgag
    69661 catttccaat gacttcagat acaccggcga cctccagagc aagacggttg agatgggacg
    69721 gattgtgagg aataaaacga aaatcttcta tcacagcgat agaatcaatg caaagaaaaa
    69781 cacctgatat gggggaataa tggcgccact cccccaacat atggaggagt ggcgaaaacg
    69841 gtgtggagga agagcggacg gcagccgacg aagctagttc gccgaggccg acacgcgcga
    69901 agccgattcg atcgtgacca gttctttggg aaggagaaat tcgacatctt cttcgatgac
    69961 cgtcagttcc tcgacctctt ccgaagagcc aagacgcttc aggtaggcca ccacttcggt
    70021 gaccaggact tcgggggccg aagccccggc ggacagtccc acactcttgg catctttcag
    70081 ccaatcggga ttgatgtccg acgccgcatc gatgagatag gagggaatgc cgcaatgctc
    70141 gcccaattcg cgaagccgat tcgaattcga gctgttcggt gatccgatca cgagaatcac
    70201 atcgaccagc ttcgacaacg acttaaccgc attctggcgg ttctgggtgg cataacagat
    70261 atcttcctgg tgcggcccct tgatattggg gaatcgccgg tgcaacgcct cgacaatgtc
    70321 ccgacattca tcgacgctca gcgtggtctg ggtcacatag gagagattct gcgtcttctc
    70381 gacatggagg ctctccacgt ctttgaccga ggacaccagg tggaacttat ccgggatctg
    70441 gcccaacgtc ccgatcacct ccgggtgtcc cgcatggccg ataaggatca attcataccc
    70501 ttgcgtatag tcgcggttca cttcgttatg caccttgatc accagcggac aggtggcatc
    70561 gatcacgtgc aggcggcggc tctgcgcctc ctcccacacc gatttcgcca caccgtgggc
    70621 actgaagatc acgaccgatc cttccggcac ttcattcagc tcttccacaa acacggcgcc
    70681 cttatgccgc agcgagttca cgacgtgccg gctgtggacg atttcatggc gcacataaat
    70741 cggcgcgccg tattttttga gcgacaaatc gacgatatct atggcgcggt cgacacccgc
    70801 gcagaatcca cgaggattgg ctaaataaat cttcatctgc tggttccctc tcacgctcat
    70861 gaagggccgc ccggcctgcc gggaaaagcg cgtagtctca ctaaacggtc accttacgtc
    70921 agacccgccc ttgtcgtcaa ccggatcatc tgacccgctc caccgcaaaa gcagttgagg
    70981 ccggtggacc attgtgggag aatcccgcca tgcctccccc cgcaacgaca cataaaaaaa
    71041 ttctgatcgt cgaagacgag aaggacattc tccaactcgt caagctctat ctggagaagg
    71101 aagggttccg caccgtctcc gcgatgaccg gcacagaggg gctccgccag gtcaaagccg
    71161 agcatcccga cctgatcatt ctcgacctca tgttgcctga actcgacggg ctggaagtct
    71221 gcaaacgaat ccggctcaat caagagactg ccctgcttcc cattctcatg ctgaccgcca
    71281 aggcggaaga atccgacacg gtcatcggcc tcgaactggg cgccgacgac tacgtgacca
    71341 aacccttcag ccccaaggcg ctggtggcgc gcgtcaaggc gctcctccga cggctggaac
    71401 ggaacgcaaa catcccccag acgcaatatc actacggccc cttgaccgtg gatctctccc
    71461 ggcacgaggt ccggctggat ggacaggaag tcgcgctcac ggccaaagag ttcggcctgc
    71521 tggaacacct gctgcgcaac atcgggcggg tgctcactcg cgacgtgctg ctcagcgccg
    71581 tgtggggcta cgactactac ggtaccacgc gcacggtcga tgtccacgtg cgccggctca
    71641 aacaaaaact tccgctgctg gacgacgcca tcatttcgat caaatcgctc ggatacaaac
    71701 tacgcgaatc cgacgtacct gcatgaacct cggcatccgg tggaaagtcg ccctgggcac
    71761 cctcgccgcc gtcttcgtcg gcttagccgt cgcggggtgg ctggtcatcc gctcggtgga
    71821 gcaaactgaa ctcagccgca tggcggagac gctggagacc agcagcggct tagccgccat
    71881 gactctccgg ccgttgttcg atcagtccgg atcgaccgta ccaggccccc tgctccacgc
    71941 caccgttcgc gacctgagcc agcaggccca cctccgcatc accgtgatcc ggcaggacgg
    72001 caccgtcgtc gccgacagcg ccgtccctac tgaagggctg actcacgtcg aaaaccacct
    72061 ctcgcggccg gaagtcgcgc aggcactgcg atcgggacgc gggatggata tccgggccag
    72121 ccagaccacc ggagaacgca cgtattatgt cgcccgcctg ctgtcagcct cgacgccggc
    72181 gcagcctggc atttccgtca tcagactcgg gcttccgctg acctcgatcg acgagcgcgt
    72241 gcgacacatt cagcaagact tagcgacggc gttcggcgcg gccttcctgc tggccatgac
    72301 cctgagcctc tgggtctcgc gcaatctcac gaaaccgctc tcagagatgg ccgcagccgc
    72361 ccgccaactt gccggcggga cgcccggcgt ccggctggtg gtctcctcga cagacgaagt
    72421 cggcctgctc gcgcaaaccc tcaaccagat gaccgaccag ctggaaacca aaatcaagga
    72481 ggtttcggac gaccgcgcgc aactgttagc catgctgatc gccatggtcg agggcgtcat
    72541 ggtgctggat tatcgcggca ccgtcgtcca ggtgaatccg gcgctggaac ggatgttctc
    72601 tctggaactg acggaatcgc ggggccggca ctatgccgaa ctcatccggc acgaagggct
    72661 gaccgccctt gtctcctccg tgctacagac ccgatccggg caaggcggcg aaattaccct
    72721 ctcgcccagc gggcgatgtc tgcgggtcga agcctcgatc gccggaggca gccgcgagca
    72781 ggaagcctgc gccgtatttg tgtttcacga catcacggaa ctgcgccggc tggaaaaaat
    72841 ccggaaagat ttcgtcgcca acgtgtcgca cgaactgcgc acgccgctga cgtccatcaa
    72901 gggctatgtc gaggcgctgc tggatggggg caaagacgag ccggcgacgg caacggcctt
    72961 cctggaaatc atcatgcgcc agagcaaccg gctgaatctg attctggatg acctgctgca
    73021 gttgtcgcaa atcgagtccg ggcaggtcct gttccgtcgt gagccggtgg acttacgggc
    73081 gctgctggaa cgaaccgtgg cggtgatcaa accccttgcg gacaagaaac atcacacgat
    73141 cgagctgtca ctcccggacg aatattgggt ggtcgagggc gatgaggaac ggctggtgca
    73201 ggtcttcatc aacctgctcg aaaacgcggt gaaatacacc ccggatcagg gacggatctc
    73261 catggcgatc cgcaacgcta cacacctccg gacgaccccg ccccgcgcga tgatcgagat
    73321 cgtcgtggcc gactcaggaa tcggcatccc tgaagcggac cggccgcgtg tcttcgagcg
    73381 attctaccgg gtcgataaag cccgctcgcg cgaactcggc gggaccgggc tgggcctcgc
    73441 catcgtcaag catatcgtgg aagcccatag cgggcaggtc tgggtggaag gcaatacgcc
    73501 cagagggagt cgattcgtgg tccacctccc cgtcgcgcaa gaatcgtcta ctgcagtttc
    73561 gacagaaaca ggtagcagat agtcgacagt agcgcagccc ccggcaaggt gaagagccat
    73621 gccgacagga ttcgtctggt cacgccccaa cgcaccgccg agagccgctt gaccgccccg
    73681 acacccatga cggtggaggt gatggtatgg gtcgtgctga ccggcaaacc gatatgagcc
    73741 gtcgccagca acacgatagc ggctccggtt tcagccgcga agccatggac cggttccagc
    73801 ttcacgattc gcatgccgag ggttcgcacg atccgccacc cgccgaccgc cgtgccgagc
    73861 cccatcgcca gggcgcagga accgatcacc caggtgggca cttccgcagt tggaagcact
    73921 ccggcggaca ccaacgccaa cgtgatgatg cccatggcct tctgcgcatc gttcgccccg
    73981 tggctgaatg ccatgaaact ggccgagacc agctgcatgc gtgagaacag ccgcaaggcc
    74041 agggcgcgcg gcacccggaa aaatacccaa ctcaggatca ccatcagcac cagcccgatg
    74101 gcaaacccga aaaacggaga caacaccatc gcctcaagca ccgagcgcag acccgagaac
    74161 ttgacggccg ccgtgccgcc gtgggtcaac gcagcaccga ccagcccccc gatcagcgca
    74221 tgagaagagc tggtcggaag tcccagtaac agggtcacaa aattccacac aatcgccccg
    74281 gccaaggccg aggccaccac cgcctgcgtc accgactgcg gatccacgat cccggttccg
    74341 accatcttgg cgacggcagt cgacatgaac gccccggcca cattgagcac acccgccacc
    74401 agcaccgccg tcgacggact caccacacgc gtcgacacca cggtcgcaat ggcattggcg
    74461 ctgtcgtgcc agccgttgga aaaatcaaag agcaatgcca gaatcacgac gaccaacaac
    74521 aacccgctca gttcagccat gaatgccgat tcctaccctt tgaatagtga tcaggattgg
    74581 tgtcgaggga gacgcggagg acgctcacgt gtgctttaac gcgatgcgct caagaatatt
    74641 ggcgacatct tcgcaccgat ccgtccccgc ttcgaagttt tcgtagatct ctttccattt
    74701 gatcacggcg atcgggtccg tctccttctc gaacaacaga gagatggcat cgcgggaaat
    74761 gcggtcggct tcattctcca agctattgac ccgcacgctg cactccgtga cggcctggtg
    74821 cgacttgccc agcaagtcca cggtcgcccc gacctccacc gccgcctgat acaacacgtt
    74881 cgccagccgg atcgccgact ccgtgggagc cgtcaccttg tagaggacaa atcggtcggc
    74941 gatggcctcg accacatcca ggatatcgtc gagggcactc gcgagatcat gaatatcctc
    75001 tcggtcgatg ggcgtgatga atgtctggtt cagcttcctg gcaatctcat gggtaatacc
    75061 gtccccgaca tgctcaacgt ccttgatgcg tttggccttt tcgacaggat tccggaaatc
    75121 ctccgtcata tctttcagga gacgactccc ctcgatcatg ttgtgcgccg cattcttgaa
    75181 caaatcgaaa aatgcttctt cccgaggaat gagaccgaac ataacgtcct ttctagccgc
    75241 gaggggttac cagcgcacca gtcctgtcgc ccaggtcaag cccccgccga atgcgcccaa
    75301 caacaccaga tcccctgccg caagtcgctg tgtccgaacc gcctgatcca gcgcgatcgg
    75361 cagggaggcc gacgatgtat tcccatactg ttcaatcacc gagtagaccg cctcctgcga
    75421 gaggccgaga cgacgacgca gctgctccaa aatccgcgca ttggcctgat gggcaatgac
    75481 ccttgtcacg tcgccggccg caagcccgaa ctcctttaag acatcgatca ttgcctgctc
    75541 caggcgacgt accgccgatc gaaacaccgc gcggccttgc atacgcagca tgtgctcact
    75601 cgcacggacg gtgtccaccc cgacgggctt ccgcgacccc ccggcgggaa tcgtaatcag
    75661 gccgtggccc gagccgtcgg catagagccg cacaccaaga atgcccggcg cgtccggttg
    75721 ggccgagtcc tcctgcacaa ccagcaccgc tccggctcca tcgccgaaga gcatggcggt
    75781 ctctctgtcc ctcgcatcga gagaccgtga tttcacctcg gcggcaatga ccaggcagga
    75841 gcgaacctgc ccgctccgaa tcatgacatc cgccatcgat accccataga gaaaccccga
    75901 gcaggaagcc gacaggtcga atgccatgac cggattcgca cccagcgcac gttggacatg
    75961 gcaggccgtc gagggaaagg cgctgtcggg agaggtcgtt gaaacaagaa tcgcatcgac
    76021 agactgtgga gttatcccgg cggcctcgca cgcgcactgc ccggcgacta ccgccaagtc
    76081 cgacgacgcc tggccctgct ctacccaata cctggtccga atcccggtca aagcgacaat
    76141 ctggtccggg tccaggccga gcggagcggc tacctcctgg ttgtctacaa cccgggaggg
    76201 cacgtacgaa ccggttccga caatgcgact gcgcttcacg gggttcctat tcactcgcct
    76261 ttggagaccg tctgggatga catgtcaggc gacctcacga ggtgcggcga gattatagga
    76321 cccgggccca ggagggtcaa cctccacaag ccgaattcgt tgcttttcgc ttcctgatct
    76381 gctagaaaaa ttgtgtagtt acgatcagtc cacgttctcg actatttacc gaaggagtcg
    76441 ctggatgcac aggcattcaa gggggttgtg tgtcggtctc accattgtgg tgggaatgtt
    76501 atggtttgca gctggatgct ccagcaaacc gaagacgacc gccgacgcca aacccgtaag
    76561 cggcacggat gagcagatct tcctcggcga cacgatcgag aagaactacg atcctaacgt
    76621 gatcatgaag cgcggcgagg cgttcttcga caaggaggag tttgccgagg ccatcgtcga
    76681 ataccagcat ttcctggaat tgcatcgagc ccatcaactg gccgtctatg cccagctccg
    76741 tttggcggaa agccatctcc ggatggccaa gtcgatcgac cgagacccgg agccgatcca
    76801 aaaggccatc gccgcgtttg aaaaactgcg gaaggatttc cccggcagca agtatgaagc
    76861 gcaggcgctc cagcgtattg ccgattgcca cgactggctc gctcagacgc acctgttcgt
    76921 cggtcagttt tactatcgtc gagcctccta tctggcagcc gcgcatcgat tcgatcagat
    76981 tatgaaagac tatccggaca agaaggtggc gccggaggcc ttgtactatt tggccctcac
    77041 ctatcaggaa ctcggtgccg atgattgggc gatggaaaaa cttcaacttc tagccgagaa
    77101 gtatccgaac agcgagaacg ccggcgatgg ccgccggcta ctggccaagc tggagaagaa
    77161 atcgccacct gcgccgaccg tcgtggccaa gaccgacacg ccggctccgc agaatcaacc
    77221 ttcgacgaca ctgcttgcgg cctcggtgcc atcgtcgagc tttacgcctt ctgctacaac
    77281 acccagcatc cccaacctga agaattcgcc ggccctctcc cttgggcaac cgttcgtttc
    77341 ctgccggctc ggcgcctggt gctgataggc gtcgaacctc ccgttcatcc acccatgtac
    77401 aggccgttca gaacacctcc cgcaagactg cagcgaagtc gacgtcgcga cgaataaggc
    77461 gggccacgcc tgccgactcc gtcgggacgg tgaggcgccg gtgtcatacg agaacgccat
    77521 tggcggacgt ctccaccagc ctgttaccgt agatgtaatg cttcgagcag caggtcgttg
    77581 accgacgcct tgaggcgctt cttcgcatcg ggcaagaatt gtacggctgt ggtacgtccc
    77641 tgcagaactt cgaaatagga ctgcaccaca tcctggtagc gtcggagaag cctgaagccg
    77701 agtcggggga aggcataaat gacccgagcg attctggccg tgactcgaaa atccgggaga
    77761 atgtcacgct ccaacgctgc ctcgtaggcg gcgagcgact cacgggggtc atgaacgttc
    77821 cccaaaatcg accgggctgc gagctggcct gaacgaaccg cgtaataaat gccttcgcca
    77881 aacagcgggt cgactaagtg accggcatca cccaccaatg ccgctcgacc attgacgaat
    77941 cgagagcctc ttccgtctcc atgcccgcct tcggcatctg aataggcggg aatcggatgg
    78001 ccaaccggac gaggcactgt ccagccggac agcccccgcg cttcgctgac aaaccgatcg
    78061 aaggtcgtgc gcaaaccgct cgatttgcga cgaaactctc ctacccccac agacacgcca
    78121 ttctttttgg ggaaaatcca tccgtacccc tgacgggcgg cccccacatc cacaaggatg
    78181 gtggtcgcgc tcggatagtg tcggctattc ccgagccgca cctcgctttc aagtgccggc
    78241 gcccgataca gggagcggtt gggaaacagc tgccgcgcaa ccagactatt ggcgccatcc
    78301 gcaccgatca ccatctttgc gcgataccgt ccctcctgcg tcaccacctc cacaccatcg
    78361 gaatcctgag tgacctcgac caccccctca ccggtccgga actcggttcc ggcttcaatc
    78421 gcttgttgcc caaggtagtg atcgaaacga tcgcgcatga ccatgtaggc gatcggttga
    78481 gaggattcaa tgagcagcgg atcctgccca cgatagacaa actggacgcc attgaccgtc
    78541 tgttcgacaa ccgatttgaa gccgggctct aagagcaggt cgatgcgcgc cgagagccca
    78601 ccgccgcaca ccttgtaccg gggatggacg tccttgtcga aaccgagcac cgtcaagccc
    78661 ccgcgacaga gagccgcagc agcaaccgct cctgccggcc ccattccgac aataatcaca
    78721 tcgtaggttg tggccaattg cctatcagcc atgcctcagc tcccagttga aggacacaga
    78781 gctccgacct ggccttggcc aagggtagac catgcactgt aacgcaggcg tcagggggga
    78841 gcccgatagg aaagggaaag ataggccgga gttactgacc gaggtaccag ccgatcccat
    78901 agcgttcgag aaagctcgtg atcatggtca gcgaattggc atagatcatc acacccacca
    78961 cgatgagcaa caccccgctc accgtcgaca caccccagag atatgcgcgc acttctttaa
    79021 agtaactgag aaatcgatcg acgccgagcg cggtgagaaa cagcggtagg gctaacccca
    79081 gcgaataaaa cgtgagcaat accacaccac tgagcatcga ttctgtcgta ctggcataga
    79141 gcaggatcgt ccccaggaca ggtcccacgc agggagtcca gcctgcagca aacgcgatgc
    79201 cgatcaggaa cgaacccaga aacccggccg ggcggtttcg gaattgatac cggtgctcca
    79261 ttttcaaaaa gtttaaattc aagataccga ggagatagag gccgaagata atcacgacga
    79321 tgcctccgaa ccggcgcaga tgctcctggt aggtaatcaa cgcctggccc aagaggctgg
    79381 ccgatgcccc gaacgcgaca aacaccgtcg aaaacccagc gatgaacagc agggaattca
    79441 aaatgatcgc tttccggaag cgattccgtt cagagacgtc cgtgagctgt tcgatcgaga
    79501 gcccggtaat gtaggaaatg tacgagggga cgagcggcag cacacagggc gacacgaacg
    79561 acagcagccc ggcggagaat gcggcaacca atgaaatcga ttgcaccgaa tcggtcatgg
    79621 ttaggacccc tgcaacagcg attcaaccag gcgcttcccc gcaggggcgc tccagtctct
    79681 ggcccctgga atccgattgc gaatgatgcc cttgcgatcg atcagaaacg tcatggggag
    79741 ctggcgtgcg ccatactgaa gcccgatgcg aaaatcggcg tcgtggagca cggggaaagt
    79801 aaagccgact tcccgctgaa agggacgggt caccgctgcg ccctgggcgt cggtcgagac
    79861 cgcgagaatc tcaaagtcct tgcggctgaa gcttcgatac agtcgctcca tcgccggcat
    79921 ctcgattttg caaggcccgc accaggtcgc ccagaaattc aagagcacaa ctttccctcg
    79981 gaactgatcg agcgtgacgt tgaacccctc ggagtcccga aggaggaagt tcggagcctc
    80041 ctcatcgacc tgaggaatac gctcgacgac aagcatcggc acagccggcg aggaaggcaa
    80101 tgctccgcga gcgtcagcag aaaaccctcc acagagcatc atgaccgcac cggccataga
    80161 taatattgag cagcgcgtca tcatcaccgt tacgacgttg gcagcgcctg agtaggggtc
    80221 gcatgggcgg ccttctccgc aggcttgaga agttgggtca acaccttaag gttatccagt
    80281 cgtgtccaat cccgaggccc gatcactttt tcacgcagga caccctgctg atcaatgatg
    80341 taggtttcgg ggacacccat caatttgtag cgcttgtcgg tctggcccca ggaatcaacc
    80401 agtacgggaa acgtgaggtt caagcccttc acgaacggag gtatttcttt cttcgtcgtc
    80461 acccggtcga tgctgaccgc gagaatcacc aacccgtcct tttcaaaatt cttgtacaaa
    80521 acctccatcg aaggcatttc ctcacggcac ggcttgcacc aggtcgccca gaaattcaaa
    80581 aagaccactt tgccgcgata gtccgataac cgcagctgct tctcgtttaa gtcgggcaat
    80641 tgaaaatccg gtgcctcttt tcccaccacc aatggttcgt acttggaact ctgtagccag
    80701 actacgagaa acatcacgcc cagaatggcc gcagcggcta ttaccaccag ggtacgggac
    80761 gggccggttc gcggcgcagt gttggcaggg ctctggttga ttggatctgt catggtctat
    80821 gcgtaggcat gcagaccgga gagcagaaaa ttcactcccc agaaacagaa aatgaccatg
    80881 agaaaaccaa agatcgcgta gatcgccgct ttatggcctt cccacccccg cgtcatgcga
    80941 gcatggatat aggcgccata gatcagccat acgatcaacg accaggtctc cttgggatcc
    81001 caactccagt acccgcccca ggcatagttc gcccacattg ctccgaggat gatcccgaag
    81061 gccagcagcg gaaaccccag catgatcgcc ttatagccaa gctcatcgat ggtttcgaga
    81121 tcgggaaaag cggcataaaa ccgcgactga ctgccacgat tgaccgcccg ttctttgaag
    81181 agatacatca ggcccaatcc accggccatg gcaaacgccg cgtagctggt caacgtgacg
    81241 gagacatgga tgtagatcca gtagctgttc agggccggaa cgagcggctc tgccgtctgg
    81301 tatcgatagg gcaagagcga cgcggcgccc atggcaataa acccgattcc caccacaaag
    81361 gcaccgacgg ccttaatccg gtagcggaat tcgagaagca cgtagcccaa gataatggcc
    81421 caggacacat aggccattgc ctcgaactgg tttgaccagg gcgcgaaggt gcctgaatgt
    81481 tccatccgct caaatgcccg cgtcgtgagg gccaacgtat tcagcaccca cccgaacacg
    81541 gtgacgaggg ttgccacctg ccccaattgt gtggcccatg cgccatcacg ttcttcaagg
    81601 tcctcaatcg gatgcccggc tggaaccatc tgcatggcag gacgttttgc gaacagatag
    81661 gccacataca accctaacgc ggctaaatac aaccagaacg tcatatcgaa taggaacagt
    81721 gatcgcatca tacggacctc cgtctgtcta tcctagcgtg cagcgcagac cgaatgaaac
    81781 gttgctctcg gtcgcgcggc agcgatcttc aggattgggc ctgagcgtta attttttccg
    81841 tcaacttccg gaactctttt tggaaatcga tctggctttt gtgcgttgtt ccgccaagat
    81901 ggagcgtcac accggactcg ccgggaatga tcttcgccca taaccgccga tgataaatca
    81961 gcgacgacag agtcatgccc accaccagca tcgtacaccc gatccagacc atattgatgc
    82021 caggatttct ggcaatctgc agcccggtga acttcttcgg ctgatatccg atgaattcga
    82081 attggtatgc ggagtccttg atctcgaaga ggtcgggata atgatagaac acccagggag
    82141 tggactgtac gctactccgc tcatcaaccg ccaaccgaat cgcgggattg gcatgctccg
    82201 cggtttttga aaacaccttc ttctcggtcg aattaaacgc gaagtcggcc acaaaatccg
    82261 tcatcttcat cttcaatgga aggccgtcga cggctttttc cttattccac tccagatcga
    82321 ccgtagcgat aatcttgtcg ctgcccttct ccttaatgtt gattcgagcc gcctcgatct
    82381 gatcccacgc atccccatag ctggattggt aaaaccagat ccctttgtag accaagggat
    82441 cgttcaccgt aatcgtcttg gttgtggtgg gagtgccctg atcgatcacg gtcaaggtgc
    82501 tgttgtagga ttttaccgaa ccgttctcat ggtaatcgat ccagaactta tcgacacgaa
    82561 gatcgaagtt gccacgcggg atatggtagg tctgcccttc gaggcatacc ccgaactcct
    82621 gaaacccata atagctaccc agtagccccc cgagcacgat gaccgtggcg ctgaggtggg
    82681 ccacatgcgc gccgacccgg cccataatcc ccttggtggc gtagaccgtc acctcgccgg
    82741 gatcgctctt ggccagcacc cgataacctt tttcagcgaa gagctttaca agtcgctcag
    82801 ccacagcgtc tctcgattga ttgagtgaga gggtggtctg ctgcttcatc ccctggataa
    82861 aggccagcga cacactgacc ttatcctgac gcatggaccg ccatacactg ggaaaccgtt
    82921 tatggaaaca agtgagagaa ttgacgcaca ggagccccag cagactggta aaccaccagg
    82981 tgtgatagac atcggtgaaa ccgaatcgca ggaaccagcg ataggcttct tctccatatt
    83041 cctgcacata ggtttccggg cgttcgccct gctgaatgac cgtgccgatc gtcgcagtca
    83101 tggcgagcac aatgaacaga aacatcgcca gcttgatcga agcaaaaaaa tccacgacct
    83161 cgcgtgaaaa ctcctcccag ccaaggccgg aggtgcgcgg agcggatgcg ctcacatcgg
    83221 atgctgcatc gggtttatcg ttcatgaatc aacataccta gctgcttcga cctggctgtc
    83281 atacaaatcc catcatcata ccaatgacga gctgcgtgtg gaatcctgcg ccaatgcagg
    83341 tcggtaccac gaggctggaa gtgccggggc ggagcgttta tgatgaaaaa tgttccaaat
    83401 gcgcgaaaag taagctatct tacaagtcgt cttaaaggac tgtcaagcaa gacgcttgtc
    83461 cggcgcgaga ctcaaccaat tccaccagaa tggtggacaa aaacagaccg gtcagctact
    83521 attgccttag ttttttgcgg agttgcctga tcatcttaag cgtagcggct tccacaatca
    83581 gtcgccgtac cacctgactt caatcgccgc atcctcgtat cacttcaatt ccagggggaa
    83641 caatgagaca taactacctg ttcacgtctg agtctgtcac cgaagggcac ccggataaga
    83701 ttgccgatca gatctcggac ggaattctag acgccatcat cgctcaagac aaattttcgc
    83761 gagtcgcctg cgaaaccatt ctcacgaccg gcatcgcatt cgtcgccgga gaaatctcga
    83821 ccaaggccta tgtggagatt cccgatatca ttcgcgacgt gatcaaggac gtcggctata
    83881 cggacgcctc ctggggtttc gattccaaca cctgctcggt cttgacgtcg atccaccaac
    83941 agtccggtga tatcgccatg ggagttgatt ccggcggcgc cggcgaccag ggcctcatgt
    84001 ttgggtatgc caccaatgaa acgaccgagc tcatgccgat gccgatcgtg ctggcccatc
    84061 gattgaccaa acgtttggcc gaagtgcgca agaaaaagat tctcaaatgg gtgcggcccg
    84121 acggcaaatc gcaggtgacc gtggaatata aggacggcaa accttgccgc gtcgatacca
    84181 tcgtcgtttc cacacagcac agccccgacg tgaccaacaa gcagatcgag aaggatttga
    84241 tggaacacgt gatcaggccg tccatgccga agggtctgta cgatccgacc agcgtgaaac
    84301 accatatcaa cccgaccggc cgattcgtgg tcggcggacc gatgggcgat accggcctga
    84361 ccggccgcaa gatcatcgtc gatacctacg gcgggcacgg cagccacggc ggaggggcct
    84421 tctcgggaaa agatccatca aaggtagatc gctccgcctc gtacatggct cgctatattg
    84481 caaagaatat cgtggccgcg ggcctggctt caaaatgcga agtccagctg gcctacgcca
    84541 ttggagtagc cgatcccgtc tccgtattgg tcgacaccaa ggacaccgag aaggtagcac
    84601 ccgaaagctt ggacaagctg gtccggaagc atttcccatt gaccccgcgc gggatcatcg
    84661 accatctcaa gctacgtcgc cccatcttta ggaagacagc cgcctacggg cacttcggtc
    84721 gcaacgaacc ggagttcacc tgggaaaaaa ccgacaaagc caaagcgttg cgcaaggacg
    84781 caggcctcta accgcctaca gcgcgtcgca ccagccaagg gggcgggtcg caaacccgcc
    84841 ccttttttct gcattcacgc atcactcgat tatcagtcac aacacaggag gacgaccagt
    84901 ggactacgac gtgaaggaca tgggattagc tgatcaggga aaattgaaaa tcgaatgggc
    84961 agaagccacg atgccggtac tccggctcat tcgcaaacgc tttgaacggg aacagccgtt
    85021 caagggcatc cgcgccaccg cttgcctaca cgtgaccacc gaaacggcca acctgatgaa
    85081 aacgctcaag gccggcgggg cagatgttcg cctctgcgcc tccaacccgt tgagcactca
    85141 agacgatgtc gccgccgccc tggttcggca cgaagggatc ccgacctttg ccgtcaaggg
    85201 agaagacaac aagacctact accggcatat tgaatcggcc atcgcccaca agccgcacct
    85261 gtccatggac gacggagccg atgtggtctc ccatctacac tccaagcgga aagacttact
    85321 ccgcaacgtg atcggtggaa cagaggaaac caccaccggc gtcatccgcc ttcgcagcat
    85381 ggccgagaag aaagtcctga aattcccggt catctccgtg aacgatgcgg acacgaagca
    85441 catgttcgac aaccggtatg gcactggcca aagcaccatg gacggcattg tccgtgccac
    85501 caatcggctg gtgtgcggct ccacgctcat cgtcgccggc tacggctggt gcggccgcgg
    85561 cattgccacg cgcgcacgcg gcatgggcgc caacgtcatc gtgactgaaa tcgatccatt
    85621 gaagggcctc gaagccgtaa tggacggatt ccgggtcatg ccgatggatg aagccgctcc
    85681 ccttggagac ttttttgtca cagtcacagg caacttgaag gttattcgcg gcgagcactt
    85741 tgcggccatg aaagacgggg caatcgtctg caattccgga cacttcaatg tcgagctgga
    85801 cattcctgca ctggaaaagc tcagcaagaa aaaactcgcc gtgcgctcgg gtgtggacca
    85861 atacaccttg aagaacggcc gtcgcgtcag cctcttgggc gaaggtcgcc ttgtgaacct
    85921 agccaccgct gaaggccacc cctccagcgt catggacatg agcttcgcga atcaggcctt
    85981 gggggccgaa tatattgtga agaactacaa gaaattggaa aagaaggtct atccggttcc
    86041 cgccgacatc gataaagaaa tttcgcggct caaactcgcc ggcatgggcg tgtcgatcga
    86101 caagctcacc aaagagcagg tgaagtactt ggcctcctgg gaaatgggaa cctagtttta
    86161 tcggagaagg ccggagctgc tctcgtggcg gctccggccc atccaccgag cagttctgag
    86221 cgtctgagcg aactcctcat accactccag acgctcctac tactctttct ctctgctctc
    86281 tataactttc tctcagtcat accgaatacc tgacctggcc tctgacaagt attggaggtc
    86341 gattgtcatg aagcgactac accacagtag gcgcaggtgc agctccggcc aaatagatac
    86401 agaaaacgtc gagtctagac ctccccacgc ctcacttgct gaagcacggg gatagctgac
    86461 agaacatggc aaaccttgcg cagactaacg tgaattgacc atgggaagtt cgcgagatcc
    86521 ttcggcctcc tgctgaatcc gctgacgctc ggctttcgac tcttccagca cactgtgcag
    86581 caactcatcg ctcagctggg tcaattgcgc tgcagcgtct ttcatctccg catgctccac
    86641 cgcccgcgac aatacttcgg ccttcgtcgc ccccttcttc tggcccaaga agggcttgat
    86701 gatgagatag gccacgtcac ggaaaggcat gaaacgcaag aatcggcgca gtagcttgaa
    86761 ggtctgaatc gggtatcggg tgagcttata aataaacagc ttcttcagcc cctcttggcg
    86821 aactgaatta atgactgccc ccggcaaaca ggtcggatca atctccgaac acttaaaata
    86881 cttgtaccaa tccttggcgt cgctcaccaa tccacgcttg atatattcct gccacagagg
    86941 cgtgccccga tagacgcaca gccgattgaa tccaaacgtg tccaaaggca atttcgaggc
    87001 gaaatcaaac gtcttctgca tgtcctcgac ggtttcgtcg ggattgccta ccgtaaagaa
    87061 accgtgaacg atttcgatcc ctgccttctt ggcgtttcta acggcggtct caacctcggc
    87121 gagcgtctgc tccttctgga ggcgatccag aattttctgg ctgccgcttt cgatgccgaa
    87181 catcaccgtc cggcaatggg cctgagccat ggcggggaag aggtgctggg ccaccgaatc
    87241 aacccggcct tcgattcccc attggattgt cagcttcgca tcgatgatcc ctttacaaat
    87301 cgcatcgatc cgcttcggct gcagtaaaaa gtgatcatcg acgaaataga cagatccgta
    87361 cccattcgcc tcgaggtact tcagctcatc aacgacgtgc tgcgcgctac gcgcccgcca
    87421 cttcccctcg ttgaaaatcg ggatgtcaca gaagacacag ggccacggac agccccgcga
    87481 agtctgcatg gtcgtaaatc gctccatcga cagcaccgcc ggcacgtcca acggcatcga
    87541 ctcgacaaag tctaattcca ggctttcccg gtcaggaaat ggccactgat ccaagtggcg
    87601 ctccatcgtc ctgttggggt tattgatcac cttgccgtct ttcgcccaag tgacaccgcc
    87661 cacctcttgc ggatcctcaa gacgatcgag caaatcaagc aacaactgct ccccgtctcc
    87721 tcgacataca aagtcgattt ctggacactg gagcttcacc aaacccgcgt tgagactggc
    87781 aaatactccg ccgaaggcca gcttgacggt actatcggcc gctcgaatct gacgagctag
    87841 aattttggca tagggatagc tggtggtact gaggaagcta agaccaacca aggccggctt
    87901 ccgccgtttg atctcctcga taatgaactc attcggggtc tcaggatgcg cctgatcgaa
    87961 catcacacat tcatggcccg accgcttcaa taccgacgac aatgacataa tgccgatggg
    88021 cggaaacccc atgacccgga tacgcccgtt cttggctcga gtcttggccg gaagggcata
    88081 aaactgggga tcacgcacat gaataagaaa caccagcata gtaacgtcct ttcatcggga
    88141 cagtaaccgg attcctcaga ctgccaccag atcgcttcga gagcggagaa tccaggttcg
    88201 aattgcagag aggccaaatc actcaggcca ttcgacccac cggaggcgtc atacagtacc
    88261 acgtctcgca ggagggaaaa agggaagatt ctgacaaaga gaggagtaca ccggtggcat
    88321 cggcgatcct caaccgttga ccggcgatat ggtttgcgcg ctacaaacgc aaagagggcg
    88381 tcggatcaga tccgacgccc tctttagaag agacacctga ctagcgaccg agcgccgcct
    88441 tcacggcatg gccgatctca gcgggattct tcacgactcg tacaccagca gcttctagcg
    88501 ccttcatttt ttccgtcgcc gtccctttgc cgcctgaaat gatggcaccc gcatgcccca
    88561 tccgacgccc tggcggcgcc gtgatcccgg caatgaaact aatcactggc ttcttgacgt
    88621 gcttcttaat atattcggcc gccttctctt ccgcatcgcc accgatttcg ccgatcatca
    88681 cgacagcctg tgtttcagaa tccttctcga acaagggcaa gacatccacg aatcccgtgc
    88741 cgttgacggg atcaccgccg attcccacac aggtcgtttc tccgagcccc aacgtcgaca
    88801 gttgatgaac ggcctcatac gtcagggtgc cgctgcgtga tactacgcca actacaccac
    88861 gtttatggat gaagccgggc atgatgccga tcttggcttc atcgactgtg atgacgcctg
    88921 ggcaattggg gccgattaat cgcacatcgc gtccatgcag cgcacgtttc accttcacca
    88981 tatcgttcac gggaatgcct tcggtgatgc agataatcag tttaacacct gcatcagcag
    89041 cttccaggat cgcatccgca caaaatggcg gcggcacaaa gatcaaagag gtatcgcagt
    89101 cggtcttctt cacagcatcg gccacggtgt tgaagacggg gatgccctca acttcctgcc
    89161 cggccttgcc aggtgtcacc ccggcgacca tcttcgtgcc atacgctttg cattgcgtcg
    89221 cgtgaaagga cccttccttc cctgtgatcc cctgcactac cactcgcgta ttcttattga
    89281 cgagaatgct cacggtcttc tcctttacgc cgccttgccg gtcagcttca caattttctg
    89341 cgccgcttcc cacagatcat tggcaacctc caacttgagg ccggactccg ccaatagttt
    89401 gcggccttca tctgcattcg taccttggag acgcaccact aatggaacgg taatcttcac
    89461 ttccttggca gcttcgatca caccatgcgc aatgcgctca catcgtacga tgccaccgaa
    89521 gatattgata aagatgccct tcacgttcgg gtccttgagc agaatccgga atccggctgc
    89581 caccgtgtcc ttcgtggctc cgccgcccac atccaggaag ttcgccggct cgctgcccgc
    89641 cagcttgatg acgtccatag tggccatcgc aagaccggcg ccgttcacca tacagccgat
    89701 gttgccgtca agcttgacat aattcagatt gttcgccgtc gcttcgattt ccagcggatc
    89761 ctcttcattg aggtcccgca ttttctgaac atcgtcatgt ttaaaaatgc cgttgtcgtc
    89821 aaatgagacc ttgccgtcca atgccaccaa ggttttctcc ttggtgataa tcaacggatt
    89881 gatttcaacg agcgccgcgt gtttttccat gaataggcga tagagattgc cgagcatctg
    89941 cacgaaggga ttgatgacag caggttccat cgtctgcaag cccaatgcaa aagcgacgtt
    90001 acgcccattg tgcccctgaa atccaacggc gggatcgacc gcttccttaa taatcttctc
    90061 cggagtcttt tcagccacct cttcgatttc catcccgcct tcggtgctgg cgataaacgt
    90121 cggccaaccg gtgtcgcgat caacaagaat gctcagatat aactctttcg caatattggc
    90181 gccctcttcg agcaacagcc gatgaacctt cctccccttc ggtccagtct ggtgcgtgat
    90241 caaggtcttg ccgatcaact ccttggcgat tcctgcaaca gcccccttct ccttcgtaat
    90301 tttgacgcca ccggcctttc cacgtccacc cgcatggatt tgagctttca ccacgaagac
    90361 cggcgtattc agttcacttg cccaggcagt ggcggcctcg ggtgaggtaa tttccttgcc
    90421 ccgtggcacc ggaaccccga actgtgcgaa caattgcttg gcctgaaatt catgaacgtt
    90481 cattgaatcc tcataagagc tagtggctca tgacaaatga cgtagagcgt gactcccagc
    90541 ggggtcggct tgcccggacc agccgccatc agccattcgc cgtacgctct ggttcttttc
    90601 gcgtataggc ggggagaatc atcggagaga tcacaccgct ggagctgccg tgctgcttgg
    90661 cctccgctcg gaatcgtcgc agatgttgga gcgtgagctt cccttgtggc atggatccgg
    90721 cgcgcgatgc cgctgtcaac cctgagcgct ttccaaacac catgaggtcc agtagcgaat
    90781 tccccatcaa tcgatttcgt ccgtgcaatc ccccagaagc ttctccggca acgaagagat
    90841 tcttcacatt ggtttccgag tttgcatcga tcttcactcc gccgttctga tagtgcaacg
    90901 tcggataaat gaggacggga tccttgctga tgtcaatacc gaatcgctca aactgcatca
    90961 tcatggcagg aaaatgcttt tccaccgttc ccggcccatg ctctgcatca agcaacggcg
    91021 tatccaacca aacaccgaca cgaccggaca tggtgcgaat accacggccc tcctcacact
    91081 cacgaataat ggaggaggag acgacatcac gggtatcgag ctcattcaca aatcgctcac
    91141 ctttggcgtt caccagatgg ccgccctccg acctgatacc ctctgtaacc aaggcgccga
    91201 tcaattgctc ggggtagacc gcgcctgacg ggtgatactg gaacgtatcg atgtgatcga
    91261 gtttcgcgcc catccggtag gagaggcaca aaccgtcgcc cgtcgcgcca taatggttgc
    91321 tggtggggaa tccttgaata tgcagccggc cgataccacc ggtcgcgaga atcacagact
    91381 tcgcagccac aaccacctgc ctgtgattgt cgagatcctt aaagatcgcc cccgtgcaat
    91441 ggccgtggtc atcactgagc aactccactg cggcgcagaa ttcgagaagt tggatctttt
    91501 gattcagcac ctcgtctttg aggactcgca tgatctctag gccagtgtag tctgagcaag
    91561 tcagaagacg tggcttagag ctcccccccc ccttcttgac gtgcagattg ccatcggcct
    91621 gccgatcaaa aagcacgccc agttcaatta accacttggc tatggaaggc ccatcctcga
    91681 ccatgacctt gagtaattcg tggtcgttct gcatatggcc acccttgagt gtgtcaagga
    91741 aatgggtgac cggagaatct tctggcgcaa cggaaatctg catcccgcct tgagccatca
    91801 cactattgga gtccccaagt cgcagcttcg tggcaagaat caccttcgcg ccggtaccgt
    91861 gggcatgcaa cgctgccgca cacccagcac cgccgccgcc gacgaccaaa acatccgttg
    91921 tgtagtgtgg ggtcagatca atattctcag cgattgagct atcaccttcg agaagggcag
    91981 cgagttcgac gaccgtcttg tcgttcgcgt tcgggccaaa tcgaatcgga cgataggcgc
    92041 tggtacggta atcaggatga tacttatgga tcagctggtc gcgatcggca ggggagaact
    92101 tcgggatagt ctgtttacgg cgagcgtccc gagtctggtg gacaatctgt tggagtgcgt
    92161 gaatatccat ggtttcactt tcagcacgga gcacagtctt cggacatatc tgcccgagac
    92221 cggcggacgg cacgaggcat tacttcactg tcgcgcagtg gtcggccagc tcctgttcgt
    92281 tcattgcgag cactttgttc cattcatcgt tgaaacggcc gtcttgaatt tccttgatac
    92341 ggatatccag cccctgcggc ttctctgtga aatgagcacc ttgcgcccgg ctcacgtaca
    92401 gggcgaccag attgggggcg atgtcggcaa tacacaccgg tgtgcacatc ccacacatga
    92461 cgcaatccat gaacatctcg gagacgctct tgaaatcacc gaacactgcc ttccacaccc
    92521 cttcacgcac atcgatcttc tgagggcacg cctccgtaca ggcattgcaa ttgcggcaaa
    92581 gcggcgcttc cggatagaga ttgaagaggt cttgcttcgg atctttgagc gactggatgt
    92641 cgtacagagc cttacgagcg gggaacggcg gcatgatggt gaaagacatg ccatcttcta
    92701 cagccatctg gcaggccagg caggtccgaa ccttgggatc atccttggtt cgatagtagg
    92761 tcgcgcaggc tccgcagaat ccgccgaggc agccggctcc acgcactact tcctggcccg
    92821 tatgccacaa tgccttgatg acggtgatac cttccggcac ttcatacttc ttaccggaga
    92881 tttcaatcgt caccattttg tggcgcaaca cttctggctg gtcgatcacg ttggtgtctt
    92941 cttgtgccat gtttctctcg tggacggctg aaagccaggc gtgaggagat tcgcttcccc
    93001 cctcacacct gacgtttaat taacggatac gcccttacgc aattgcgtcg atgatagcat
    93061 ttagcgtggt gcttgggcgc atcgccttgg acgacttcgc gtcatcaggg tggtagtacc
    93121 cacctgtatc gaccggctta ccctgagccg caatcagctc cccagatatc ttggcttcgt
    93181 tatccgccat ttccttagca accttgacga agcgcgctgc caaattctta tcctgcgtct
    93241 gctgggccaa cgcctgcgcc cagtagagag ccagatagaa atggctgccg cggttatcga
    93301 tttctccgac tttgcgcgcc ggcgatttat tgctctccag gaacttggca ttggcttggt
    93361 caagggtatc cgccagcatt ttcgctgctg gattgttcgc cactttggcc agatgctcca
    93421 gggaggcggc cagcgccagg aattctccga gcgaatccca ccgcagatag ccctcttcct
    93481 ggaactgctg cacgtgcttc ggtgcggatc ctcccgcacc ggtctcgaac agaccaccgc
    93541 cgttgagcaa cggcacgatc gagagcatct tggcgctggt tccgatttcg agaatcggga
    93601 acaggtccgt cagatagtcg cgcaacacgt tccccgtcac ggaaatcgtg tccttgcctt
    93661 ccttgatccg ttccagcgag agacgggtcg catcggccgg cgtcatgatc cggatatcga
    93721 gtccgttcgt atcgtgttct ttcaaatact gattcacctt cgtgatcaat tgcgcgtcat
    93781 gcgcccggtc cttgttcaac cagaaaatcg ccggggcgcc ggtcgcccgc gcgcgagtca
    93841 cggctagttt cacccagtcg cggatcgggg cgtccttcac ctggcacata cgccaaatgt
    93901 ccccctcttc gaccttgtgc tccaacaacg cttttccaga cgcatcgacg acgcgaatcg
    93961 taccgttgcc cggcgccttg aaggtcttgt cgtgtgaacc gtactcttcc gccgcctgcg
    94021 ccatgagtcc gacgttgggc acgctgccca tcgtcttcgg gtcaagcgcg ccgtgcttct
    94081 tgcagaaatc gatcacctcc cgatagaccg gagcatagct ggcatcggga atcacgcact
    94141 tggtgtccgc cagcttcccg tccggtcccc acatcttgcc gctgtcgcga atgaccggcg
    94201 gcatggaggc atcgatgatg atgtcgctcg gcacatgaag gttggtgatc cccttgtcgg
    94261 aattcaccat cgccatcggc ggacgctgct tgtacacagc ctggatatca gcttcaatcg
    94321 ccttgcgctg atcttcaggt aacgccttga tctttgcata gagatcgccg atcccgttgt
    94381 ccggatccac gcccagcttc ttgagcgtct caccgtgctt ggcaaagacg tccttgtaat
    94441 agaccgtcac agcatgaccg aagatcttcg ggtccgagac cttcatcatg gtggctttca
    94501 tgtgaagcga gaacagcacg ccttgtttgg ccgagtcctg aatctgttct tcgaggaatg
    94561 tgcgcaacgc cttgacgctc atgaaggtgg catccaccac ttcgcccgcc tgcaaggcca
    94621 ctttcggctt cagcacggtg accttgccgt cttggccgac gaactcgatc cgcgcttcag
    94681 tcgccgcggc cgtcgtcaat gacttttcat tcgaaaagaa atctccaccc ttcatgtgac
    94741 tgacgtgcga cttggagtct gaactccagg cgcccatctt gtgcggatgc ttcctggtat
    94801 gcgctttcac ggacaagggc gcccgtcgat ccgagttgcc ttcgcgcaag accggattga
    94861 ctgcactgcc tttgacttta tcgtaacggg acttgatatc cttctctttg tcgtctttcg
    94921 gattttccgg atactcgggt agcttgtagc cctggctctg caattcctta atggctgcct
    94981 gcaactgagg cagcgaggcg ctgatattgg gcaacttgat gatattggct tccggtttct
    95041 tggccagttc gcccaattcg gacagcgcat ccggctgccg ttgttccggc gtcagatact
    95101 caggaaatac cgcaagtatg cggccggcca gcgagatatc ccgcagttcc accgatacac
    95161 cggctgcctt gctgaaggca ttgatgatcg gcaaaagcga ataggtcgcg aacatcggcg
    95221 cttcatcggt cttggtatat atgatcttgt cagctttcgt agtcatgatt ctccctctta
    95281 ttcccccatg agttagttca gcgcattcag cgctgaacct gccttgaacc aagcgatctg
    95341 ctgctcagtg atgctgtggt tcgtttggat ggtcagagcg ttaccatccg ccttgtgtat
    95401 ggtgacctgc acaggtttgc ctggagccag attgttgagg cccgtcacgc tgatgcggtc
    95461 ttgctgctcg atcttgtcgt agtccttcgg atctgcgaag gtcaacgcca cgataccctg
    95521 cttcttgaga ttggtctcat gaatccgagc aaagcttttc gtgatcacta ccttcacatt
    95581 caaaaatctc ggagacatag ccgcatgttc acgactgctg ccttcaccgt agttctcgtc
    95641 acctactaca actgacccta tgcctttgga cttgtagtcg cgtgcaatct gagcaatcgt
    95701 gagatttgcc tcacctgtga gcacattcgt tcctctgccc ggctctgatg taaaggcact
    95761 atttgcgccc aaaaacatgt tgtcgctgat cttatcgaga tgcccacgga acttgagcca
    95821 tggcccagct ggagaaatat ggtcggtggt ggtctttcct tttgtcttga tcagcagcgg
    95881 tagcttttca aagtctttcc catcccagcg tgggaatggt tgcaacagtt gcaagcgctc
    95941 gctggtaggc ggtatgtcca ccgtcaaccc ctcaccgctt tcagcaggag ccacgaaccc
    96001 ctcttcacct ttcgcaaaac ctttggctgg aagttcctca ccttgtggcg gctggaattt
    96061 gaactccttg ccgtctgcac ccttgagcat ctgattcacc gggtcaaaac caagatctcc
    96121 cgtaatagca tacgcggtca cgacctcggg gctcgccaag aacgagagtg tttcgctgat
    96181 tccatcattg cgaccaggaa agtttcggtt gaaggagcta acgattgaat cagcctttcc
    96241 cttcaccccg tcggcccgct tccactgccc gatgcaggga ccacaggaat tcgagaggac
    96301 cgttcctccg agcttttcaa acgtatccaa aaacccgtca cgcttcatcg tatgataaat
    96361 gcgctcggat ccaggagaaa caagaaacga tgccttcgct tttaaaccag ctttcaagcc
    96421 ctgctcagcc acatgggcag agcggctgat gtcttcatag gaagaattgg tgcagctacc
    96481 gatcagtgcc gccttcagct cgaccggata ccccttcgcc tttgcctccg ccgccaactt
    96541 tgaaatcggc cgtgcaagat ccggcgtatg cgggccaact acatgcggtt cgagcgttga
    96601 cagatcaacc tcaacgatct gatcgtaata tttctccggc gattgataga cttccggatc
    96661 cgccacaagc aggtctctgt gagattgagc aaaggtcgcc agatcagcgc gatccgtgat
    96721 ggtcatgtag gcgaccatct tctcgtcgaa ggggaacaca gaggtcgtcg ccccaagctc
    96781 cgcacccatg ttacagatag tgcccttgcc tgttgcactg atcgtttcgg cccctgggcc
    96841 aaagtactcc acaatcttgt tggtcccgcc cttcactgtc agcagaccgc acaggtagag
    96901 aatgacgtct ttcggcgaag cccagccatt caattttccg gtcaggcgca cgccgatcaa
    96961 tttcggatga agaacctccc acggcaaacc tgccatcact tcacccgcat ctgcgccgcc
    97021 gacaccgatc gccagcccac ccaagccacc accgttcggc gtatgtgagt cagttccaat
    97081 gatcaaacag ccggggaacg catagttctc caacaccact tgatggataa tcccggctcc
    97141 tggcttccag aacccaatgc cgtacttctt tgccgcagaa gcgaggaaat tgtagacctc
    97201 tcgattttcg tccatcgcgc gaagcaggtc cttctcggaa cccatttcag cacgaatgag
    97261 gtgatcgcaa tggatcgtgc tcggcacagc cgctttttcc ttattggcct gcatgaactg
    97321 cagcatggcc atttgggccg tggcatcttg catggccaca cgatctggcc tgagcgccaa
    97381 catcgccttg ccccgctccc aggtctgcgc gtcgaaatta tctgcgtgcg agaccaagat
    97441 tttgtcagcc agggtgaggc ctcggccgaa ttgctttctg gccttggcaa acacttccgg
    97501 catcttggca tacagtttct tggcgagatc catcgacatg acttatctcc ttaaaatatg
    97561 tctgtcacca aatcagcacc atcagccggc cgggaacatg acattccaac gaccggattg
    97621 acgagtcatg tttgacgacc gaccattgtc gtcttacttc aatggcggaa cttcccgccg
    97681 cttcggtgcc gcataattca caaggtagtc gaataaacgg atcagccggc tatcctggcg
    97741 tttttgatca acccagtggc cgatcaatcc gatcgtccgc gagagaatga agaacccgtt
    97801 caagctatcg acagggaatc cgatatcgac cagtacggct gccatggtcc catcgacgtt
    97861 cagaatcaga ttatcctttt tcacggaggt aattttctcc acctccagtg cgaagtccag
    97921 gcatggtgtc ttaatattca agctcttcac gtacccaacc aattccttca ctcgcttgtc
    97981 cgggttgcgc aaactcttca cgcgatgacc aattcctgga accggcccat ggtttttctt
    98041 catgtaggcc aagaattcgt cgaccgacat cttgttatcg acagcatact taaagaaccg
    98101 cccggcatca gtaaccgcac cgccgaagcg cggaccaatc ataatcatgc ccgcggcaac
    98161 tgattgcgac agcccgatcc cggcacaagc cgcaaggatt gtcgcgtatg ctccgctcac
    98221 gcaagggccg tgatcagcgg aaagcatcat gatgcgctta atgatttcgg cttcctgttt
    98281 cgagatcagc cgcttgtccc acaaaagacc gatgacatga ggaatttcgt agcctttgtt
    98341 gatgagctca gaggcaggat aaccgtcgta gcagggctca tcgccgcggt catcgctgat
    98401 ggtcgtacgg atcagcggcg agaccatgac ctcgtcagcc ttcatcgcct cttcgatggt
    98461 ttttggcaac cggggcaacg tagccggctc gaccggttcc ttgatctggc ctgacttcaa
    98521 gagatcttga taggtttcct tgatggcagg accaagggcg ccaaaagtgg gcggcacgag
    98581 ggcgccggct ttcttcagcg catcggactt ggagcgagct gatccttcac ccttcatccc
    98641 ctctttcgcg ccggcatgac cgaacttcat ccccttcggc aaactctcct gacaaaatcc
    98701 ggagaccacg gcaatcaact tcacccggcg ctttttcgcg ccataccatt ctgcagcccg
    98761 ctcttcgaga tcaccgccca tttcgccgac gatgacaacc gccttcgttt gcggatcgtt
    98821 ttcgaacatc tcgagataac tgacataatc ggttccagga taggcatcgc cgccaatacc
    98881 gatggccgtc gtgatcccgt cggcaaactg agagcagatc cagatgattt cgttcgagag
    98941 accgccggac ttggtgatga caccgaacga tccttcgcga taaagcttgg atagaacaag
    99001 attgtcgaac gcaccgccaa tcacgccaag ccggcatgct ccggcggaga tgatgccaat
    99061 ggatgaggga ccgttaaaga ccttccccaa cttgcgcgca tgagcgccaa gaatcttggc
    99121 atctttttcc ggcacgccct cggtgatcat tgagaccacc ttgatatgag aatcgtccag
    99181 cgcctccata ccgcccttca tcgcacggtc tgcgccaatg tacacgagac tggtattcag
    99241 ggtcggatgg tgcttggtgg cttcagcaat ggacttgtag accggaatgg cgatcagacc
    99301 gctgccgtat gggatctcat tgctcttgcc agcatcgggt ggatagacaa acgcctcaac
    99361 gttgagcgga cgtttaatga gataacagaa ctcggccatg cggcgggcag cgttgacacc
    99421 ggcttgacca ccctggatca ccacacgggt gtctttgttt gccagaatac tcatctcggg
    99481 atctcccctt atcttcggtt ggtcataggc ggcaggctac ttcgcttgga gtgccttgtc
    99541 gacaatgtcg gtgagcggtg tattgcggtc gaagacattg atgtcaaagc cttcttcttt
    99601 caacgcgcgc atcgcatcaa gcccctcttt ttcccgcggc ccaccgcgac gcacccaaat
    99661 ctttacgctc ttcaacttcc cttcggactt cgccttcctg aagccgttga tgatccctcc
    99721 gaacgtcttc ttcacgtcgg tgaaattggc gatcgcacca ccaacgatga tgttccggat
    99781 tcccggcaga gaacagacct tttccgtgag gacctcgacg gcccaatcgg gtggatcacc
    99841 ggagtattcg gcatagttgg ccaacttgcc gccacgcgcc acgaccgcgt cggagtagta
    99901 cacgctggca ccaccgcccg ccggcaacat cgctgtatcc ccaccaggaa tttctataaa
    99961 tttaactgag cctttaatct tgctgtcgac agccatcacc tcgagctctt gcttggaata
   100021 ggcacggcca aattccgccg caaaagcgaa gttccagtcc ggatgtctaa atttggcatc
   100081 gccgtcgagc aatgtcacgg catcaagcgc caccagttct tcatcggcgg cacgaaggac
   100141 gaccggattc acctcgagat actgcgcatc ttcactgtcg aagcaggcaa acattttccc
   100201 tgcaaactca gccatcttct tcgcgagcgg cccgccaaat cctgcctctt tcgacagttt
   100261 ttcgagggat tcaggagaag gagcttgtcc gatctcaaga cacaaccgct taacacgttc
   100321 ccaatttgat tcgacttcga tcccgccaca attggcaacc aggatctcac tgccttcgcg
   100381 cgttgatttg accgcgcaat aatactcttc cttgtgcggg atcatctcag agacaatcac
   100441 ctgagtcacg gtgatgctgc cgacctgacg gccaatcatc tctttagtgg cagcttcagc
   100501 cgccttgaag tccagatcga tcttcaccag ccccaacttg aagcgcgacc cgagagcctc
   100561 gtgtgccttc gcaacaagct tggacttctt caaccactca ttggcttgac ccagcctggt
   100621 caactcgtct acggaggtga cgaccacata gttgggaaca ttgattcccc acttcttcat
   100681 cagccccatc ccgggacctt ccagcacttt cgccatgatt gacgactcct tggtgtgtga
   100741 tagggcagag aaacggagcg gtagagtacc gaattctgct gattagctca atcctccaaa
   100801 aggcccaagc tgcgagcaca gaagtcctac cataatgagc actgaagatt catcaccata
   100861 aataattgat aacgttatta ttgtttccga cgcttcaagg tttggggtta gcgagacttt
   100921 tcaggccttg tggcgcgccg acgcccgttt cgctggcatg tgggacagta aaacgaactt
   100981 cgttcgccga tagaacgaca gatcacagca ccgcaggcat tcgggcaggg ctccccggct
   101041 ttggcataca ccagatgcct gcgcttgtac tggccttcgg tcccatccgg cgcaaaataa
   101101 tcccgcacac tcgacccccc catggcgatg gcttcccgta acacctcccc catgacttga
   101161 tgcaaccgcg tgatcgactt ccctggaatc gaattcgacg gctgattggg atggaggcgg
   101221 gaacggaaga ggatctcatt tgcatagatg ttgccgatcc ctgcgatgac ctgttgatgc
   101281 atcaggagag actttagccg actccgtgtc gcccggagca cacgcaggaa tttctcatga
   101341 ctaatcgtaa ggggatcatg gccgaaccgg cgagcgacat atcgatccag tcccgcccgg
   101401 tcaagcagcg ataggcgacc aaaccgccgc ggattccaat atctgatatc aggctcacaa
   101461 caaccatcga ggtgcaggat aaagtgggta tgttgggggt gcttggtcaa ggtggatcgg
   101521 aacagcaaca atccggtcat gcctaactct gcgacgagaa atctcggctc cttgccgcag
   101581 agaaagtgaa gaatcacgct tttccccttc cgctcgaccc ctgcaatttt ggcctgacga
   101641 tactgatcca gagaaggcaa cccttctcgc acgatgtcct cacggccaat ccagcaatca
   101701 cggactgtag ctccgacgac ccgttcgcgc aattgacgcg cggcgacttc ggcttctggc
   101761 aattcaggca tcgagcattc tcgaggtggc agagagaacc tactgaaggc aaattacgcc
   101821 atggggccga ggcaaaatgc aactccgacc tgagatagtc caggagacat caatttggat
   101881 gactataacg agagcgtccg gagagggatg aggaactgac gaagaaggac aaatcggaat
   101941 gtccctgaca gcgcagcttc taagacgttg atgaattcac ttgggtttgc cggcggcgac
   102001 aaacatcctg aatcgtttct tggaggcgcg gcaacggcat gccgcctgga cgcctggtca
   102061 acacaagctt caccgcatca gcatacgcca tcccttccac gcaacagaga taagcgatca
   102121 ccacagaaac cgaacgtccc atgccgaccc gacaacacac catcaatcga ttgtccgcca
   102181 catgagccgc gatccattcc acagcctcgt ataaagccgc agctgtcggt tcaccgaatt
   102241 cctttaacgg gatcttggca tagataaccc gggagggaac tgtcacattt tgttcttcag
   102301 ccaccatgag aacggcactt acctgggggg gaggattcct ggcgtcatcg gcattgccga
   102361 ccagaagacg tttgttgatc atgtgcatcg tgtcgttagt atagggagca tagactcccg
   102421 gtcaagtgaa tcctcggaat ctgcgcaggc ggttgaagat cattcaaacc gatgcgtata
   102481 ctcaattgct ctagcttagg gggaactgat ggctcacacg tctcttgaag tggtagaaaa
   102541 acggctggcg agtgtccagt gcgcaatctg taagaccaat accttcggtg tcgaccggcg
   102601 cacattgcag ccagatggag aatgccgggc ggtctgcttg aaatgtcggt acaattttcc
   102661 cgtctacaca gacatggagt tttaccttcg gactcaacca gatgtgccct atcgcctgaa
   102721 agagatctct tgcccaagct gccagcatcg aggcgtcagt ctcgattttc gcatcaccat
   102781 gtccgtccgc gaggcgatct acttcgtgac ctgcagcgcc tgcaagacac agtttcctga
   102841 gcgctcgtca ttggaagcgt ttgagtagcc cttacgaacg caatttcgtg tatactgagt
   102901 ttattatgag caccacacca actccagatc ccccgaaagc ccctactccg gtcactcagg
   102961 ctgaaactaa gccccgcaag gaaattcctc tcatttcagt ccctaacgat cgggacatga
   103021 ttgcggcgga aatggccgaa ctcttcgatg aagatcgggt gtcccatcag cacggctcct
   103081 cagaacccga agctgactag atcttcacgc aggacgaaca atctccacta cgccggagga
   103141 ggtaatgcgg ccgaccatag ccggtctccc ctttcgctcc ttcctgacaa tttcacttac
   103201 ccgaataatc gccccacgat gccagtcatc gaaggccgcg ttcgaacttg agctttcctt
   103261 gtcctggcgc ccaatctcgg acctccccgc ttgatcgcac aaccagaccc gtttctcgcc
   103321 cttcatcaca cgcaactcac tcaccacgcg gtggtagtcc ggatctcgtg gcacgagcgt
   103381 ttttccgtcc ttgcgaaaaa tcacatagga aaatttgagt gcatctttga gaagcccgac
   103441 ttcccgatca acttgagcaa tccaggctgg agtcttccac ttccgttctt catggcacca
   103501 gtcatccggt ttgagtagtg caggacaagg agcgtcatgg agacagggcg caaagacaga
   103561 acagtgctgc cctgccagaa ggttgtcccg cacctgatgc aattcgcgcg aagcaggccg
   103621 cgtggccggc tcgataagca ttagactccc ttcaggggcc aagtaattca ggagttcccc
   103681 taccaacgca gtccgttgtc ccacgggatc catactccct acgaataact ctgagagaag
   103741 attctgaaca attataagct ggtaaccatt cacgccaccg ccgtcacgga tagcggaggg
   103801 cagggaacgt tcgaggttac actccacggt gttcaacggt gggatggact gatcccgctg
   103861 ctgttcacaa tacgcccgcc aaatatcagt agacaccctt aagacatgag gtgaccgatc
   103921 aacggctatc atacggaggg tagacggcgg cctcgcagac tttgaaaggc accaatcgag
   103981 cactcccagg actcctgttc caggcccgcc accgaggtcc aatactcgaa aatcctggtc
   104041 ctgagcgacg ggaagcaccg gctgcagttc gtccaagagt atctgcactt tggcgagatt
   104101 aacaggcaga tagtagacca agtaaccggc acacaactga gggtcctcga agtaaaccct
   104161 gcttcccacc tgcccatttc gtgtaaacat ttctgaaagt cccgacaccg cctcaattat
   104221 tgattgggat ggcccctccg agtgctcaag gagatttact ttggccacct tcgtgatagc
   104281 cctcaaaact gccgatgata gttggtacat agacgaaccc tcggttgacc ccccgagcag
   104341 gctggggtat tctcatgaga gaaggagcac ttcacatgac tgaccctatt ttgcaagcat
   104401 atctggaagt ggagatggcc atggagcgct tcacgcttgt cctgcatgac catgtcgatc
   104461 atctccgcaa gactgaagcc cctggctcag acaagctgca ccgtatggcc aacggcacca
   104521 aagccatgcg cgatagcgca tcgatttacc tatcctacgc caagtacgtt gctcacggga
   104581 tgccggaaag ccctgatcta gtcgaggagg atcttcaggg ctgaggagcc gcactcaagc
   104641 atctccccaa atacacaggg cccgttcggt ataaccgaac gggccctgct gtttctaccc
   104701 tgacgttact tctcggccta aatgctgcgc atgaaatgcc ccatggcctc atgatcagca
   104761 gctgatgcgc ccataacttg tgaaatcgca acgttcgtgg acttctttcc ggtcagaccg
   104821 gactcgactt cgtccacgat caattccacc ggcatggact gcccaccgta cactctcggg
   104881 ccaccgataa tcttggcatt tgagaaaccg taaatagcgg ttgccacttc ttttgctaac
   104941 catccgacat agttgaattc aggcacaaca attaatttgg ccttcccgca gagtttgcgc
   105001 aactcctgag tcgggaatgg gcggagcgat cggatcttga tcagtccgat cttaatgcct
   105061 ttttcggcgc aaatacgaac cgcttcacgt gattgagcgg ccgcactacc tgaagcgata
   105121 ataaccgcct ccgcatcatc cacattctcc gccgtgagta gtccacccat gtactggttg
   105181 atgtatttac gggaacgctc cacagcagcc cacacttcct gctgccacac cgcgtgaatg
   105241 ttgtaggcca tgaaattgga cttctgaacg ggagcatcgc gagagagtcg tgccggagga
   105301 ttctcagcat caagcaccgg caccgcccca cgccaaggtt cgcgtggggg aagcttcatc
   105361 ccacgatctt gcatacgcac atagccacga gcatgcgtca caaaaaaccc atcacagcac
   105421 acgccgacgg ggagggtcac atcattcttc tcgctgataa taaacccagc caacgtgaag
   105481 tcgtacatgt cctgctgatt ttcggcatgg aacacaatca tgccgcaatt gagcagataa
   105541 gaaacttcga tattgtcagg ttgaatggcc aacggtgcgt tcaccactcg gcaagtgaac
   105601 atggccacga ctggcaaccg atgccccggc caagaagcaa tcccctccag ccctctcagc
   105661 gtccctggac ccgccgttgc agtataacac cgcactcccg cccgtgaccc gccggcgata
   105721 gccgccatga cgccgacctc ttcttcgcca cggtagtact ccttgacgta cccttcacca
   105781 tacaacacac cgaccaactg catagtttca ctttgagggg taatggggta cgcaatggca
   105841 agatcgacgt ttgagcggcg aatagcctct tttgcggcct cgctgccagt gatgaactcc
   105901 ttcgtgcgag gagcttccag gaacatatac tcaggagtga caactttctg ccgctttgca
   105961 tccgcgtgcg gatccttctt ggtctcggcc ttcggagccg acacactcgt aatagggtcg
   106021 ccctgtggat tggtcttttg tttgggttcc aatgcttcac tcatcgttgc acctcttgac
   106081 tgtatcgcct tagcctaaaa ccttagcctt cccgcttcat ctgctcagct gagtggccaa
   106141 atgtagccgc gctgcctacg cctgcagcgg aaggcataaa ggtcatagga ttcgtatgcg
   106201 tcttcccatt atggaccttg gactgagttc cggtgaatcg cacgttctca ccgttatccg
   106261 gcaacttaat gcccatttcc tcacggacct tcttgaagat ttgcgctgtt ctcttcaatt
   106321 tcaacgtgtc cgaatcgcga attgtcagca tggcgtcctc atgcccctgc tcagcacaaa
   106381 tggccatggt gcagcagttc gtccctgcac gaatcatttt cagcgtaaga ttggcatagt
   106441 gacagtgaac gccgatgaaa atacaggcct caatcttgtt gccccagatc gtgagattgg
   106501 ggtgattggg attgatgacc tcttcagggt caattttggg gtacttggga cgataatccg
   106561 gcatgggaat aatcatcacc tcagggatct gtgccgcaat ttccaataca gccttcgcct
   106621 tctcgacagc atggttattc caagcccaca agacgagagg tcccggaaaa atcgttgcat
   106681 tcgggcttgt cagcattttc cgagccatct cttcaatcac tatgtcttcg ttaggttcca
   106741 gcagcccata gtacagtcct tcgcctggat caggcagcgc gacccctaat tgagctgctg
   106801 atggaggatg gaatccggct ggccccggca cgataattct ctctcttgta tcttgcgtca
   106861 ttcccacgcc cactcccctt tcttacgctt acccgactac tgggctcgcc aagaccgctg
   106921 tcgtactctt atcgctatat gtgatgttat cggtcaacgc ggccatcggt aattgatcaa
   106981 tcatgatcat tttaatggca ttgttcttcg ccatgttgtc gcagacccac acacattgcg
   107041 cgcatccttt acaacgatct acggccacgt atgcagaccc gtacttaaag cccttctctt
   107101 tccccatctc ctcgctatac tgaatggtat tagcctcagg acaatactga gtacagagct
   107161 tacagctctt cgaggcacaa atttcaacat cgatatgggc gactaaatac attggaactc
   107221 cttcgtacta aatcctggtt aggcgctcac agtcgcacgg atttccgacc gtttgaccgt
   107281 cggtgcagcc caaccgtggt cgactgcgta gttccagccg gcctgaataa ccgcgacatt
   107341 cttgtcgatc aactcctgct tctttttaaa ttttcgctcg acgacactgt ccagggccgc
   107401 ggttcctccg gagaccacaa atcctttacc aaggaaccga tcctttaccg actgctccag
   107461 cgcttcaaca gtagtcaacc cggtgatcgc tccgatgcaa cccatcaagg ccatattcgt
   107521 gcacaagtcc atgcccgcca cttcgaggga tagcttagta gcgggaaaat agtaaagcct
   107581 tgcacgacgc tcttctaact cacgagcctg atcccgatga agcttcatcg gaccatcatt
   107641 attgatcaac gcgattccgt cttccttcaa tccgaagtaa aacggcatcg tatacgattt
   107701 tccgtgagta atgacctgtg gatggaaaat gataatgatg tgcgggaagg tgatctctcc
   107761 gatttcgtag atgggctcgt ctgatactcg cacataactc tccaccggag ccatgcgctt
   107821 ttctgatccg tagaatggaa ctatcgtact ttccccaccg gcattaatca ccgcagtact
   107881 caggatgtgc gagccggtca caaccccctg gccacccaca cccgccatcc gaatgttgaa
   107941 acgtttcgcc atgctcaatc ctccttaaac gttgctcagg ctttcggaag cccaggagcg
   108001 ggagcagccg gcttcgggag aaattccttg tagccatacc gttccgtaag aaattgcttc
   108061 gcctcgtcgg taatatattc ggagaactga tagcgatcgt tttcaacgtt cttcgcgtct
   108121 tccatgacct tgtcggtcgg aatggcatac tcaatgttgc aggacgtgta cgcctgaatg
   108181 taagtcgatc ccacctctct ggcgatcaac acggctttct taatgacgct ctcaacacgg
   108241 cgaggattgt tcggcaccac cgtcgcgacg taggcgcagc ccgcaacctt cgccatctgc
   108301 agcatatcca tcttctcaaa cttcttcccc aacggagcca tcttgagcac ggcgccgcga
   108361 ttcgtcatgc cactctcctg cccgccggtg ttcccataca cttcattgtc tagcatgatc
   108421 gtggtgaacc gttccttacg gaaccaggag tggaggacct gctgaaatcc aatatcagcc
   108481 gtaccgccgt cacccgccat gacaaccaca tctttcggct tatcgccaaa acgaaggcgc
   108541 aagccacggg acaacccgct agccacacca ttctggtcac catagttgcc gtagacgaag
   108601 gggatggcgg cttgcgaaat cgccaagcgc ccgcaaccgg ctgttccaac agtgatagta
   108661 tcttctggat tggggaaggc aatcatggcg agtcggataa agagggtcat cgcacaaccg
   108721 gcacacatgg ggtgctcttc aagcacttct ttgaaggagc ccatctgtga aacggacacc
   108781 tttttgccga aaggaccatg ctcaaccatg tcccgatatt ctttcggcat gaacttttca
   108841 aagccgcttg aaaacttgac gtaatcaagg ctcataagga ctccctccct tttataagct
   108901 gaggactttg ttcggaccaa caacagcggc cagaaaaagc caggtgcaga ttatcttgac
   108961 gtcatattgg actaaacacc gaactgtgct cctgacacgg aacggaatgt tagtcttctt
   109021 acttcgtgta gtcgccgagt gtaacaaagc ccgaaaaaat ctgtcaatcg caaccagcac
   109081 ggcaaaagca aaagatccca tcacttcagg acaatggcga gtctatagag tccacaaaag
   109141 cattgcaact ttgcagaagg cccacaccgg acgaaagaag gcctagtaga gatacccgtg
   109201 ctaggccctt ggccctaaat ccgcttgaac gcgaagattt tactcctctg caagtccgat
   109261 ctgtcacgca attcagttgc agttcacccg agaagcgggt aaacattgtt gcttaacgcg
   109321 tttcggtcta cactccgcct atgcctatac gagtactggt cccgggagaa gaacatggcg
   109381 agcagcatgc gggaggggtg cacaagcgac caccaattcc ggatggctcg ctcaaggccg
   109441 agtgcccaaa attcatgagc catggcccct gcggaggcgt ccgcaaaggt ggattctgcg
   109501 aggtctaccc ggaaatgacc tgtccgtggg tcacgctcta caataaattg aacgaactgg
   109561 gccagctgga gtggatgaag caagtctgac aacaagaagg tggggggagc gggtgacggc
   109621 gaaaggcagt aaaaagcgga agccagctag ggcaacgaca aagctgggat acaacgagcg
   109681 gcacgccaag agtggaatct ctgctcctct acccgacatt gagacattcc ccaatcaata
   109741 taagggatac gaaattacaa tcgtcatccc agagtacaca gccatttgtc ccaagaccaa
   109801 cctgccggac ttcggaacta tcacccttca ctataaacca gacaagcatt gcctcgagct
   109861 gaaggctctc aaaacgtaca tccatgccta cagaaatgta ggaattttct acgagaatgc
   109921 cgtcaaccgc atcctccagg acatcgtacg agcctgtcga cccatcaagg ccacggtaac
   109981 cggtgaattt gctgctcgtg ggggactgcg gagcgtaatc gaagcaaaat atccggcata
   110041 aattgcctac tcaacagcct ccactttacg ccgtcgacgg gctacttatc ccgcttcacc
   110101 tcatcaccag cacacccatc gcaactatcc cagtaacact gcggcagctc cctgcgcgct
   110161 acgcattacc aatgtttctc gctaaaaccg cctaaagtcc cactcaaaat ccaccgacat
   110221 cagacagtca aggtgcggca cgggtcggca caggctaccc gcacgtcaca acagggtcgc
   110281 ttccgagcag gaaaaggatg cccgtcttgc gcacgcaatc catgggagcg accatcgttc
   110341 aggcggtggt aggactcttc gtcgttatat cgggaacgta cgacatgtcg gttcaaaccg
   110401 tagaagctat ccccgccttt gcacggaaat acgcgttgaa ctgcacgacg tgccatacgg
   110461 ctccaccaca gctcaataca ttcggtgaac ggttccttga aaacggctat cagcttccgg
   110521 gaaccgaaga tgggggcatc acggggaaga agaatctcgg cgacctcagc ctggatgatt
   110581 ttgcgaacta cacaggtgtc cggctccgtg gaaacgtcct acgcacccac aacttcaagc
   110641 agcagaatcc tccgggcgca gaatccggcg cggtccaaaa caagagcgag ctcggattcc
   110701 cggaagtgtt cagcctgttc actgccggca ccttgagcaa aaacattggg gtttttgccg
   110761 agctggaatc caatctcgaa gaagggacaa ccgggatgga acgagcgttc attactttca
   110821 acaatgttgg aggtgagaat atcgccaaca tccgcatcgg gaagctcgac ccttcggccg
   110881 cattctcctt ttccaccctc cggcaacaac tcgaattaat cggtgagagc acgaattcct
   110941 ccaccgatgc ggtccagcgg gcagggctct tcccacttgc cacagccgct aaattctacg
   111001 gtctgcgcga tcgctccgga gccgtcattt ccccctatgc cccctcgctc tacaacacag
   111061 tggcggaaac cggcatcgaa gttcgcgggc gtccgttcgg cgactggttc atgtatcagg
   111121 tcggtatgct gaacggctcg aacgagggct ttggagattc caacaaaggc aaagattttt
   111181 atggcgccat ccgccttgat tacgcccgct cggccaactt ttcagcaagc ctttcgggat
   111241 ttgcctatct cggaaacagc aatgccaccg tgttcgacgg cacacgcact gtcgatgtga
   111301 attggaatcg ctacggcgcc gccgcgcacc tacgctacaa gatgctggat ttccatggga
   111361 tgtacacact ggatcgaatc aagcatgtcc caacagcggc cttgagcaat ttcgacacca
   111421 ccgccagcgg agtgaccgtc gcgctggatg cctacgtcac gaaccgaacc ctgctctcgc
   111481 tacggtacga caacatggat gccgggggag atctgaccca gcgcacatcc cagagtttcg
   111541 ccggcatgca actcaagcac tacctacgct cgaatgtggc cgtttacgcc cgacacgacc
   111601 tcaatttgcg tcgggcagaa gagggcaatg cggcagccca caacctgaga catgcagttt
   111661 ttgtgggaat cgatatctct tactagaccg ggaagaatca tgcatcgcca tgtactccga
   111721 tatcttgcga tcctcatcgt gacgggcttc tgcgccacga ccgcgcaggc gctactgccg
   111781 aaggacgggc gggataccat tacagagggg catcgaatct atgatcgggc ctgcgcctgg
   111841 tgccatggca gcgaagggaa aggggacggg ccatccggct ggtccatcgg tcgctacgca
   111901 gcccctcgtc ctcgcgactt caccgcggag agctacaaat ttcgaagcac cccctcgggt
   111961 gaactgccca cggatcagga ccttttcaga accatcacac agggtattcc cggcttcatg
   112021 ccgtcgtacc gatccttgaa cgagcaggaa cgatggcagg tcatcgccta tctcaaatcg
   112081 ttgaatccgg cgttcgagca ggaacagccg acgccgatcg cgctcccctc tccgccgccc
   112141 cttccctccg ggaatggaat cacaaacggc cgggcgctgt acgccaagta cgagtgtcgg
   112201 acctgccacg gggacaacgg tgcaggtgat ggaccggaat cgaaggccgg gcacctgcgg
   112261 gacgcccaca accttcccat caccgccacg gacctgaccg acccatcctc attcaaaaac
   112321 ggggcgacgc ctcacgatct ctatcgaagc atcatgagcg gactcgacgg cacccccatg
   112381 cctgcctatg ccgatcaatt tgccggacag gaagaagcta catgggatct ggtgtggtac
   112441 ctccagtccc taacaggtca accggggcga tgattcggca ccatgaagca cccacatttc
   112501 atacaacctg cgaatcggac gcccaacggg gaaatggaga tgcggtacat gaaaccgata
   112561 ctgctgaccg tgacactctg cgtaatactc ggcccgagcg ccctcctgag agcggaatct
   112621 cccacaagca cactggtagg ccgaatcacc tatgccggcc cgatcgaatc agggcaggtg
   112681 gtcgcggtca cacgcgattc gtcgttctgc ggcgccacca ccgccatcca caccgtgacg
   112741 gtgaatgcga agacgcgagg ccttcaaggt gcagtcgtca gcatcgacgg cgacacagtc
   112801 cccatggccg attcgccacg ccgcccgcta gtcctcacca atacccactg tgccttctcc
   112861 cctcgtattg tcgcaggtga ggttggacag caactggaga tccgcaacga cgaccccgtc
   112921 atgcataaca cccacatttc catggggccg agaacctttg tgaacgtcgc catgatcccc
   112981 gcgagccgac cggtcgaaaa gccgctcaag cagccaggca tctatctagt caaatgcgat
   113041 gcacacaaat ttatgcgcgc cactgtgctc gccttcacgc accccttttt tagtgtcaca
   113101 gacgacaccg gcgcgtttca gattgcccaa ctcaccccgg gagagcacgt tgtgagggta
   113161 tggcacgaca cattaggaac gttccaacaa cgagtcacga ttccaacccg cggcgaagcc
   113221 accatcacga tcgaataccc cgctcatgtc gatgccaata aaaaatagga ctgactggtt
   113281 ctgccgcccc tggcgcatct ggcacgggaa cctccgcctc cgtctcagcg tcggaatggc
   113341 ggtcctgatt acgatcggca tggccggttt cgcgaccttc cggttaattg aaatccgtca
   113401 gtccatcgaa caggccacac aagcccgggc gctggcggtc agccgcacgt tttcaatggt
   113461 gggcgccgcc gccgtgttgg acaatctctt tcgcatccaa gaggcgttag gtcgttatgc
   113521 ccaggattcg gagattctga gcatcctcat catcgatccc gacaacatga tcgtcgcagc
   113581 gacgaatcct cagcaaatcg gacagcagct gacggatgag accctcgcgc aggcacacga
   113641 aaccaacgcc gagaccattt cccatggcca gactatgggc ggcgtgccga cactcctagt
   113701 ggccacgccg ctcctgaacg agcaggacat cgccacctgg gtgcggatcg agttttccct
   113761 cgcggcgatg caggaggaac tcacccgcga agcgcgccgc ctcttcctcc tgtcagtctt
   113821 attcatgggg gccacgattt cggtcgctca actcgggatc aggcgcatgt ccggcctgtt
   113881 tcgagaaact gccaaaaagc tgcaggagac cctccatacc ctccattgcg tctcggaaca
   113941 ggcgccctcc ggcgatgaga catccgataa cggcaactcc cctgcctcac cgtcctgcaa
   114001 gggcgaactg gagcaagtag tcgatctggt tgagacgacc accaccctgc tcaccacgca
   114061 ggcacaacgg ctccaatcct tcaccgactc actggagcag gctgtacggg aacgaaccat
   114121 cgaattgcat caggcaaagg aggcggccga ggcggcgaat cgtgccaaat cacaattcct
   114181 cgcgaatatg agccacgaaa tccgcacccc gatgaacggc gtactgggga tgacggaact
   114241 cctcctcact acaacgctca cgccgaagca acgttcacta gctgacaccc tgcaccggtc
   114301 cggcaccggg ctcctggaca tcatcaatca gattcttgat ttctcgaaga tcgaggccgg
   114361 caaactgaag ttggaaatga tcgagttcgg cctcagacag acggtcgaag atgcggtgga
   114421 tctgtttgca gaaaccgccg gtcgcaaaca cctcgaactc acctgtttcg tgccgccgaa
   114481 catcccggac accgtgatcg gagatcctgt tcggctccgg caaatccttc tgaatctcgt
   114541 cggcaacgcc atgaagttca cccacaccgg cgacgtggcc gtcacgctcg aactcatcac
   114601 ccacaccacg acgaacctga cgctgaaatt ccgcgtccgt gatacgggag tcggcattcc
   114661 cgaagcagca caagcacggc tgtttcaggc gttctcgcaa gccgataact ccaccacacg
   114721 gaaattcggt ggcacggggc ttggcctcgc gattgtgaaa caactcgccc tcctgatggg
   114781 aggcgacgtc ggcgtcgaca gcgtgtccgg ccagggatcc accttctggt tcaccgtcga
   114841 actggaacgc gccgcccagc gtacggccgc ctcgggagca accgaaagac cgttagacgg
   114901 attctccatc ttcatcgtcg atgacaattc gacgaatcgg caaatccttg aaacgcactt
   114961 gaccgcctgg ggcgccgcag tgctgtccgc cacctccggc ctggaagcgt tggcgttgct
   115021 cgatcgccaa tgcaccgccc ggcgcagaat ccatctggcc gtgctggaca ttcacatgcc
   115081 ggacatggac ggcattacgc tggctcggac cattagagaa gatgcgcggc tttccggcat
   115141 ggggctcgtc gcgctcagtt cggtcgacca ggtcacagac gaatcccagc ggtacccgtc
   115201 gctcttcagt gcctggctga gaaagccggt ccaccagtcg ttgttgaaag attgcctcac
   115261 acgcctgtgc caccacgtcc cggcgaccga ggcacccgcg ccgacccagc ccgacgatca
   115321 cgccgtccca ttcgccgccc acgtcctgct cgcggaagac aaccccgtca accgcgaagt
   115381 ggcattcacg atgctggagc tacttgggtg caccaccacc atggttgaaa acggccggga
   115441 ggcggtcgag gcgctcacga atcggccatt cgacctggtg ctgatggatt gccacatgcc
   115501 ggaaatggac ggcctgaccg ccaccgccct gattcgtgaa caagaaacgc aggccgcgca
   115561 gccccggcac acgaccatcg tggccctcac cgcaaatgca ttggaaggag accgcgagcg
   115621 gtgcctcgcg gcaggcatgg atgattatct gtccaagccg tttacattga ccacgctcaa
   115681 ggaagtgctg caccgccggc tttcgatcaa ggggccctca cgaacaaccg aaccccttct
   115741 gcggtcgaca ccgcaggcac cgcccccaca ggcggcacca ccgaccggac ccgccgcgcc
   115801 aggcgacgac catgtggacc tcaatgcgtg gcaggcgatc cgtgctcgcc aacgacccgg
   115861 ccagccggat cttctccaca aggccctctc attgtatctg ccccatgccg acgcccaact
   115921 tattcaactc cagcaggcca tggccggcgc cgacatccag gctgtaaaaa ccatcgccca
   115981 tatgatgaaa tcttcaacgg cgcagctcgg cgcaccgcga ctggccgacc tgtacgcgca
   116041 aatcgaagca acatgccggg caggcactgt ggacaatctt cccgatctct gcgggcattt
   116101 gattcctgaa caccgggcgg tatgcaccct gatgcgtcaa gaactcaacc gggcaggacg
   116161 gtcggccgca tgatgcacag tctgaaggac aagccgtgac gatgcgtcgg ttcctgcaac
   116221 gactccggca gagcgtgcgg cgcacaagcg ggcaactcgc gaccgactcg ggcgactcgc
   116281 aaaccgaaca ccgggagatt ccgccttcca acacgcctct gtccctcgac gaaggccgcg
   116341 cgctcgacgc cgtcctctgc tcgctcgacg aaggcctgtg catcgtcgat gcgcaatggc
   116401 acatcctcag actgaatccc caagcggaga tcctcttcgc cgcccccgcc catgacctga
   116461 aaggacgccc cgcgtatcaa ctgattgctc ccggcccgga ggaattccgc cacgagtgcc
   116521 tcgtaaccga ccgctccatt cctccgctgc aatccggact cccctaccgg accgacaatg
   116581 gtctcctgct cagacaggat ggccagttga cgccaatttc tctgacgatc actccgatgt
   116641 cgcatgatgg cgtcgtacgc ggagccgtcc tgacgttccg ggatctcaca gcccagaagg
   116701 aggcggagtc acatcagatc gaaaactccg cgttactccg ccgcttacag acaggcatgg
   116761 ttaggttggc gagcaatcag gcgatctacc gtggacaatt gaccgaggcc ttccacctca
   116821 tcacccgggt cacgacacag agcctccgcg tcgcgcgggt cagcatctgg ttttatgcgc
   116881 cggaccatgc ggtcctccgg tgcgcggacc tctatgacca accgggcgat caacactcac
   116941 acggcatcga gcaacccgcg gccctttatc cacagtactt ctcatcgctc gaggcgggag
   117001 agatgatcgt cgcgaacgag gcgcaggcgg acccgcgaac ggtggaattt gcagcacacc
   117061 atctgctgcc gctggggatc acctccatgc tgaacgtgcc tatccgcgcc gagggcattc
   117121 tcgttggggc gctgcactgt gaacacatcg gaacgcccag agtctggacg acggaggaac
   117181 agcagtttgc ggcctcagtg gcgaacaccg tctcgctcgc cgtagaggca gcggaccggc
   117241 ataaggctga aaccgaaacg aaacgtgccg aacaatttct cgactcggtg atcgaaaacc
   117301 tccccatcat ggtctatgtg aaagaagccg aacagctccg gttcacgcgc tggaatcggg
   117361 cggcggagga gctcacagga ttcagtcgcg aactcatcct cggccggacc ggccatcatt
   117421 ttttccgcaa agaagaggcc gaccgcttca cacgtcatga tcgcgaagcg ctccggagcg
   117481 gccgcctcat cgaggtcgct tcagaagaaa ttcacacgca gcaccggggc gtacgactgc
   117541 tgcgcaccaa gaaactgcct gtcatggatg agcatgggcg cccccgcttc ctgctcggca
   117601 tcgccgaaga tatcaccgac cggaccgcgc acgaatcgag tctgcacgag agcgaagaga
   117661 agtaccgcga gctgttcgag tcctcgcgcg atgccatcat gattttgtct gcgccgaatt
   117721 ggaatttcac ggcctgcaac ccggcgaccg tccagctgtt cggggcacgg gacgccgagc
   117781 atttcacgtc actcggaccg tgggacgtct cccccgagca tcagcctgac ggcgaagaat
   117841 cgatggtcaa ggcgccgaag atgattggga tagctctgca ggaaggctct cacaacttcg
   117901 agtggttgca caaacgagtc ggcggtccca gcttcctggc gagcgtgcag ttgacccgca
   117961 tcacgttgaa agggcccccc ggactgcagg cgaccgtccg cgacattacc gaacaacgcc
   118021 gcagcgaagc cgcactgcag gcctattcga cattccagaa ggccatgctg gacaacgccg
   118081 gtcatgcgat catctcctgc aaacccgacg gcatgatcca agtcttcaat ccggcggcgg
   118141 aagccctgct gggatacagc gccgacgagt tggtcggcaa acagaccccg ggcatcttcc
   118201 acgacttgga cgaagtggtc gcgcgagcac gtcaattttc caaggaatta ggcagcacga
   118261 tcgaaccagg cttcgacgtg tttgtcgaaa aatgccggag gaatctcccc aatgagcacg
   118321 agtgggcgta cgtgtgtaag gatggcaccc ggaagacggt gctgttgaat gtcacggcac
   118381 tgcgggaggc ggatggccgg atcaccggct acctcggcat cgcctccgat atcacgccgc
   118441 ttaagttggc ggcacaggag ttggtttcgg ccaaggaagc ggccgaggcg gccaatgtgg
   118501 caaagtcgag gttcctggcc aacatgagcc acgaaatccg cacgccactg aacggcgtcc
   118561 tcggaatgac cgaactgctg ctgagcacca gcctgtcggc caaacaacag tcgctgctgg
   118621 aaaccgtccg ccgttcgggg gtggccctac tcgaagtcat caatgacatt ctggatttct
   118681 caaagatcga agcgggaaag cttgaactcg aacaggtgga gttcggcctg agacaggtcg
   118741 tcgaggaggc agtcgagata tttgccgggt ccgccggcaa caagaaactg gagttgacct
   118801 gcttcatccc tgccgacgtg ccggatagca tcgtgggaga tcctgtacgc ctgcggcaaa
   118861 tcctgctcaa cctgatcggc aacgccatca agttcacgac acagggagag gtggcggttg
   118921 cgtttgcgct gacgaaacaa catgcggata cgttcacact ggcgtgccag gtacgcgata
   118981 ccggcatcgg cattcctgac gccgctcagg cccaactgtt cacggcgttc acccaggccg
   119041 acggctctac cacccggcga ttcggcggca ccgggctcgg cctggcgatc gtcaaacaac
   119101 tggtacagct gatgggtggt gaagtcggcc tggtgagtgc gcccggccag ggcacgacgt
   119161 tctcgttcac attcaccgtc acacgtggaa gtgctcacgc cgcataccct ccgcttgccg
   119221 aacgccatct cgccggacgg cagatcctga tcgtcgatga cgcacgcaca aacctcgaga
   119281 ttctggccac ccacctgcgg agttggggag ccacggtctt caccgcggag tctgccgacg
   119341 atgcgctcct ccaattggag cacttccggc acacgcagca tcgaatcgac atggccatcc
   119401 tcgaccttcg aatgccgggg cgggacggcg tcgatctcgc gcgttccatc aaacagcaga
   119461 cggcttatca gaacctcccc ctgatcgcac tcagctcagt ggagcgtctc tcggacgaca
   119521 gcgacgtaac ccaccggctc tttcatagct ttctgcgcaa gccggtgcga cagtcactgc
   119581 tcaaaggccg cctgacgcag gtgctcggag gctccgccgg aacagtgacg ccaagtccgc
   119641 aatcctcagc ggccccgcga ccacgcgtcg aagcccacat cctcctggcc gaggataacc
   119701 ccgtcaacca ggatgtggcg tcctccatgc tggagatgct gggatgtcgc atcacgatcg
   119761 cacccaacgg acgggccgcc gtcgaggcag ccacaaccgg ccggttcgac ctcattctca
   119821 tggactgcca gatgcccgag atggacggtt ttgccgccac agcagccatt cgccggcaag
   119881 aagcgacagc gagcgaccgc cgccatgtgc ccgtcattgc gttgaccgcc aatgccatgg
   119941 aaggcgatcg cgcccgttgc ctggccgccg ggatggacga ctatctcacc aaaccgttta
   120001 ccgtcgcgca gctacacgcc gtcctcacgc aatggttgac gccacaacca gccaaggcaa
   120061 gcgagaccga ccaggcctcc tccccccata ccaccgacgc cgacgaaccg gccgccacag
   120121 aggaagcacc ggtttcgaca accatcgaca agactgcctg ggatgcgatt ctgtcgctcc
   120181 aacgcccggg acggcccgac atcttagccc gcgtgctggc gacttacttg gacgattccc
   120241 gcctgctggt ggagcagatt cgctccgccg tgcagtcaca ggatgctgtc gttctttgcc
   120301 aggccgccca ccgcttgaag tccagcagcg cccagctcgg cgtcctggcc accgccgccc
   120361 gctgcaaaga attggagaca cttggacggc ttgctcggat cgacgaggct gctcaccttc
   120421 tgtcgcaact catcgaagcc catcagttcg cctgcaccgc gatcacctcg gaactgcaac
   120481 agcgctccgc ccgataagcc aactgttcga ccggctctcg cgacttgcga atggacgtcg
   120541 tccgcgctag tctgcacgca cctatgccta cctatgcaat cggcgatgtg caaggctgcg
   120601 ttgcctcgct cagacacctc acacagacga ttggattcaa tccggcgcag gatcgtctct
   120661 ggttcgtagg ggatctcgtc aatcgcgggc cggattccct cgccgttctg cgttatattc
   120721 gtgggctcgg accggcagcg gtcaccgtac tcggtaatca cgacctgttc ctgcttgccg
   120781 ttgcgaccgg cctggcgacc cttcgccgcg acgatacgct gacccaagtc ctggacgccc
   120841 cggactgcga cgaactcacg acctggctca gacagcaacc gttgctctat cgggagggtt
   120901 cttatgtact ggtccatgcc gggctcttgc cgcactggac aattgaggag gcccagcaat
   120961 tggccatgga ggccgaaaca gcactgcgtg gagaacaatt cagcgcaacg ctccgggccc
   121021 tccaccccag cggccattta caatgggcgc ccgatctgaa aggcccggtg cgccttgcca
   121081 ccatcatcaa agtgttgacc cgaatcagaa cctgctccga cgacggtatc atggaatcga
   121141 ccttctccgg cccgccgaaa ctcacacccc agggatttca cccctggttc gatctcccga
   121201 accgacgaag tgcgactgcc acgattgtgt tcggacattg ggcggcgatg ggcctgcatc
   121261 tcacaccgaa tctgtgtgcc ctggacagcg gctgcgtcta tgggcggcag ctcaccgccg
   121321 tgcggctgga ggaccggaag gtcttccaag tgcgttgcga ggagacacgt ggaggtaaat
   121381 gagcccccgc tcacggtcga ttggtcccgc tacagtagca gggcgctgcc gacctaccga
   121441 ttcctcccgg gcctgactcc acatccacgc cgcgatccac tggggcattc cttcgcagaa
   121501 ccggagccac gcccgcgccc ttttgcacag caccaatggc cagattcaga agactatttg
   121561 tatgcggtcg atctctataa cttcgggtac tggtgggaat cgcacgaagt gttggagggg
   121621 ttgtggcacg cgtgcggacg cagaacgacg gcgggcaact atttccaagc cctcattcaa
   121681 ttcgccgccg cccatctcaa acgcgcgctc ggcaatgagg tagccgcgca caaccttgcc
   121741 catagcgggc tcctgcgcct gcagaacagt cccgcctcct acatggggct tgataccgtc
   121801 acattcgccc atgacatcac atcgtggatg ggggaccaca gccggccagc cgtgcgtatt
   121861 cgtttggaaa gcttcggcaa cgggccatca gtgaacgagg ccccctgagc aatccatcgc
   121921 tcctggtagg gtcaggaaca ccttcgatgg ttcaagccag gtccgccgac aagtccgagg
   121981 tgagaagtaa acagcatcgt cgctgctggc aacggtaagg attgattcca gtcgggatgg
   122041 aagttggggg gaacggcaac gggctagtga taactttcag aatcggacgg gaaggtgccc
   122101 tgctcaacct catccttgta ccggcggaga gcctgcagag catcgacctt gagatgcgcg
   122161 tagggtttga cgaacttcgg cacaaactca tcgaatagac ccagcaggtc atacagcacc
   122221 aacacctgac cgtcgcaatg cgggccggcc ccgatgccga tcgtcggaat cgtgagtgcc
   122281 tccgtcacgg tcttcgccaa ccccgcggga atcgcttcca gcacgatccc tacggccccg
   122341 gcagcttcca acgcctgcgc gtcttcaagc agttgctgcg cccgatcctt gcccttcccc
   122401 tgaaccttat acccgccgta ctgatggact gattggggcg tcatcccgag gtgccccatc
   122461 accgggacac caattttcac gatcgccgac acacggtccg caaccgcagc tcccccttcc
   122521 agcttcacgg catgggctcc ggcctgaatg aaccggccgg cgtttcgaag cgcctcctcc
   122581 tggctgacct ggtaagacag aaagggcata tcgccgatga ccaacgaccg ctgagcggct
   122641 ccggctacaa gcttcgtgtg gtacagcatc tcctccatcg tgacggagag ggtattcgac
   122701 ttgccctgta ccaccacccc cagggaatcg cccaccagga tcacgtcaat cccggcctgc
   122761 tcgaccatgc gcgcaaacaa cgcgtcgtac gcggtgacaa ccgcgatctt ccgcctgtcg
   122821 cgcttgcact tctgcaagtc gggaacggtc attaccccag ctctgctttc gtgagaattt
   122881 ccggcagccg ttccgtcgac ttgatcgcca tgatgtgcac tccgtcgcag tacggccgca
   122941 cagctttgat ggtgcgcacg gcaatctcga caccgacatc gagtgcccgc ttgtcccccg
   123001 ccgcgcgcat ttcgtcgatc atgtcctgcg gcacacagac gccgggaatg ttggcgttca
   123061 tgaattctgc catcttcgcg ctacggagca acagaattcc ggccagcacc ttgaccttga
   123121 atggacgcac cgcctccatg aatttcttga actgctccgg ctgataaatc gcctgggttt
   123181 gaaagaactc cgcgcccgcc cgcactttga cctcgaactt gaccaacatc gggccgagcg
   123241 gatcggcttc gggagtcacg gccccgccga tcgtaaaagc ggtggacccg tcgagcttgt
   123301 tgcccgcata gtccttcccg ttattcaatc ccgtgaccag ctgcatgact tgaacagaat
   123361 ctaggtcgta caccggcttg gcttctttat ggtcgcctac ggtgggatag tctccggtca
   123421 gacagaggat gttccggatg cccagaatgt gcgcccccat gagatcggat tgcatcgcga
   123481 tccgattacg gtcccgacag gtcagctgca tcaccgggtc atgccccaac tcatacaaca
   123541 cacggcagac cggcaaggac ccggcacgca cgatcgcggc cgtgttgtcg gtgacgttca
   123601 ctccatgcac acgtccgacg agcgccttgg cactctcgac cacatgggtc aggttcgtgc
   123661 ccttcggcgg gttatattcc accgtcacgg caaattgtcc ctgggccaat acgtctttga
   123721 gtcgccgcgg ctctcggctc atgcgagtct cctatacatc tcgatcctta cctgcccttc
   123781 tccactgtcg caacggacaa gccgctggcc caacgagggc cttccggggc cgcgcgtgat
   123841 gcgatgcgag ggatgccggg catcacgtgg gcgctcaccg gcgagcaaat gagccggcag
   123901 cctctcgatc aaccctattt catccctgcc attctcttag cagattcaac cgtattgtcg
   123961 agcaacatcg caatcgtcat cggtcccact ccgccgggaa ccgggctgat ccacccagcc
   124021 ttctggctga ccggctcgaa atccacgtcg ccgcagagct tgccgtccgg cagcttgttg
   124081 gtgcccacgt cgatcacgac cgcaccctct cgcaccatat ccgccgtcac gaacttggct
   124141 ttcccgatcg ccaccaacag cagatcggcc tcacggcaga ccgccgcaag gtccttcgtc
   124201 ttcgaatggc aaatcgtgac ggtggcattg cgttggagca acatcagcgc caacggtttt
   124261 ccgacgatat tgctccgtcc gacgaccacg gcgcgcttgc cttcgatcga cacgccagcc
   124321 gattcgatca tcttgatgac ccccttcggc gtacaggctt cgaagaccgg gttgccttcg
   124381 actagccgtc cgaagttgta ggggtgaaac ccgtcggcat ccttattcgg cgagaccgcc
   124441 tccagtacca cgcggctctc gatgtgcttg ggcagcggca gctggaccaa gatgccgtga
   124501 atcttcggat cggcgttctt cttttcgatc aatgccagca gctctgcttg cgtcgtgcta
   124561 gccggcaact tgtgatcgtc cacatagatc cctgccaatt cgcaggcctt ctgtttattc
   124621 ctgacgtaca cgtgcgacgc aggatcgtct cccaccaaaa ttgtcgccag tccaggcttg
   124681 actcctgttt tggccagcac cgcggccgac tctacagcca gtcgttcccg gacttgctgt
   124741 gccaacgctt ttccatcgat aattcgagct gccacgatat cctccctggc ctgaatagaa
   124801 tcaaaaagtg tcggcaccat agcaggcaat ttttcaacaa gtcaacgctg gcggctccgc
   124861 gtagaccacc gacaacgccc cgttaggaag cgatattcca ccgctcctct cctgtcgcag
   124921 ccacttcgca atcagtcgcg cctccatgca ccgacctgcg gcttccttga cagtctccga
   124981 ggcccttggc tacgatgcat ctacagaatc ctctgtgcga caccttcgtt catgaattcc
   125041 aggaccatcc gccacaacag attcacacgg ctactgcgcc gccggggctt cggcctggcg
   125101 ctcgctctgg ccattggagt cctgttccat caaccctggg cctgggctct tcaagtgagc
   125161 ggactggagt ctccgcatag ttttctggcc gacccggcga cgaggtccta ttacatttcg
   125221 aacatcaacg gggaagccga tgcccgggat aacaatggat tcattaccag gctgagcgac
   125281 gacggccgga tcaccgcctt caagtttatt gagggcggcc gcggagacgt cacgctccat
   125341 gcccccaaag gcatggtggt cgtcgatgac gtcctctatg tcaccgatct ggatacccta
   125401 cgcgccttcg acaagacgac gggtaaacct ctcgccactc tggcgctccc acgccccgca
   125461 gccggcggcg ccgcccagtc cctcactgat gtggcctccg atggccaagg ccacctctac
   125521 ctggccgacc agggcggcaa cgcgatctac cgcgtggatc tagtgccgac attgacgttg
   125581 tcgttgtatg tctccggtgc gcacttggcc ggcccctccg gagtcgccgt gcatcccaag
   125641 accggacatg tggtggtggt gagttatgat tccggaaaga ttttcgacat caccccggaa
   125701 ggcacgctca cagaactggc atcgaacgga ttttttaccg cccgattcca gaatctcagc
   125761 ggcgtggatt tcgaccggtg gggcagcatg tatgtctcgg atgggacgaa gggcaagatc
   125821 tggcgcatgc tcccgaatgg aaagtttcaa gtgatcgcgg agtacctgcc gggtccatcc
   125881 gatgtgggaa tcgaccgagt caaccacctg attctcgtcc cctaccaaga ctccaatgcc
   125941 gccgaggtca acggcctcga atcgccgacg gcttcatcgg gtgatcgggc caaacgcaca
   126001 ctggcggact atggattcgt cgaacccaag aaatccgaca aggaaggatc tcctcgcaaa
   126061 tgagccgatg tgtctactgc aagcaacgca agggcaaacg ttcctgtccc gccctcaccg
   126121 gtttgatctg cagccaatgc tgcggggaac atcgactgac gagaatctcc tgtcctcctg
   126181 actgcgacta tctcgacacc gggagcgact accagcagaa gcggctgggc gagcagtttg
   126241 ccccggtccg gcgggagctc taccggcaac tgagtgtggc gggaggcgaa aaggcggccg
   126301 cgctgttcaa cctcatcgag gtcgtcacct tcggctattt ccacgaccgg cgggacggcc
   126361 aggatgccga agtttttgcc gccatccaag cccttcgtcg gaccttgagc ccccttcatg
   126421 tgccgtcggc cccgatgccg gtgtttgcgg aacgactcaa gaaggaatac gacacgttcg
   126481 tcaaacaaca gcctcaacag gtgagtgatg ctggttcagc cccggaaatt ctcgatcgcg
   126541 cggtcgcgat cctcacagag ttttcaggcc aggactttca atcacgccgc ttcctgaacg
   126601 gcatgatcgg ctacgtcaaa accttccacc ccgagattgc agagcatctg accaaacagc
   126661 acgaagccgg ccggatcgtg ctcccgggcc aactgacccc gcccgcccag gagccgaccc
   126721 atgttcacgg tccagattgc caccaccact aagcccgcgt cgaatcaggc cggcatccgt
   126781 cactgccccg ccatgctcgt caccacccgc tcctattaat tcctgtacct ccctacgctc
   126841 attttctctt tagtttcggc tgatttgtac cgatacaggc gtgccaagac ctacgactgc
   126901 gcgaaagagc gcggccaggc gaatcgccac aactaactga aactcatggg ctctttcgtt
   126961 atggatctgc tggcaagtct gccgaaggga gatgcactgg ccgaaccggc atggaactcc
   127021 cgtcacaggg gaattctttc gatactctgg ctccacgtgc tgagcgtgcc gatgttcggt
   127081 atctacatgg gggcaagtcc ctccctctat ctcgggggag gagggctcct tgccttcatc
   127141 gccgtcgccg cacggctttc cgttgtccgc cgccgtctcc aatctgccat cgccacctgc
   127201 gggctcatga cagcttcggc cctcctggta cacctctccg ggggtcaaat cgaactgcac
   127261 tttcattttt ttgtgatgat gtccgtcatc gtgctgtacc aggattggct tccgtttctc
   127321 gtcgggctcc aatttatcat cctcgatcac ggagtggtgg gcacgctcat gcccgacatg
   127381 gtgtacggac atgcccaagg gcaaacacac ccctggacct gggccttgat ccatggcggt
   127441 ttcatcctgg ccgaatgtgc ggccctgctc tacttctggc gggtcaatga actcgcacaa
   127501 aatgaggtcc ggcaaagcga ggccagaact cgcatgatca tcgaaaccgc cctcgacgcc
   127561 gtgatcacgt cggatgccac ggggaccata accgggtgga acacccaggc tgagttgatg
   127621 tttgacgtcc cacggaccga ggcgatcgga aaatacctga ccacctacgt atccgcctca
   127681 tacccctcaa ctgaaccagg ccccatcact ccgtacgcgg ggttagtccc cggagccatc
   127741 ctcaaccggc gtagtgaaat gctgggccac acctgccagg gaacaagttt ccccgtggaa
   127801 atcgccatga gctgtctgac cgtcggcggg acgcagcagt tcaccatttt cgtgcaggac
   127861 atttccgagc gaaagcaaca ggaagagcgc ctccatcgcg cgaaggaggc cgccgaggct
   127921 gccagcctgg ccaaatccca attcctggcc aacatgagtc atgaaattcg aacccccatg
   127981 aacggcgtcc tcggcatgac cgaactcctc ctgacgaccc cgctgaacga caaacaacgc
   128041 cgatacgccg acaccgtaca caactcggga accagcctgt tgcacatcat caatgacatt
   128101 ctggatttct ccaagatcga agccggccgc ctagtcttgg aatacatccc cttcggtctc
   128161 cgtcagatgc tcaccgaaac catcgggctc tatcacgaac aggctcgaaa aaagggactg
   128221 acgctgagcg tgaccattgc gccggaatcg ccggacatgg tccacggcga tccgcaccgg
   128281 ctccgtcaga ttctgaccaa cctggtcggc aatgccctca aattcaccga gacgggaaat
   128341 gtggcggtct tcgtgcgacc ggacgatggc gatcagggac gggtgcgcat tgaggtaacc
   128401 gatacgggca tcggcattcc gctgcatgct caggaaaaga ttttcgactc cttctcgcag
   128461 gcggacggat cgatgacgag aaaatacggc ggcaccggac ttggactcgc catcgtgaag
   128521 caactggtcg gcatgatggg cggacaactc ggcctcacca gcacacccgg acaaggcacg
   128581 accttctggt tcacgatctc cgtagacagg agcggctcac ccgagacgga gtcgcagccg
   128641 cacaggtccg atgagaccac ccccacgatg tccacacgac agcaaccaga caactagaag
   128701 gagatccctc acatgcatgt cgagacttgc gctatcaaaa ccggcagttc cttctctcct
   128761 gcgtcgctct ccacactgga ttcggaggaa accctcgtcc tgctcttcgg agcccgggac
   128821 ttgatcgata cgccgcatcg catccgcgag gtcgtcgacg cctgcccccg gagtcacgtg
   128881 atgggctgtt cgacggcagg cgaaattcac ggatgcgaaa tttttgatga cagcatcgcc
   128941 gtcgccgccg tgcgcttcga ccacaccccg atccgcacgg cccacgcggc agtgcactcc
   129001 ccaaacgatt cgtacgccgc cggacgcgcg atcgccgcac agctcagaca accttcgctg
   129061 cgaggcgtcc tggttctgtc cgatggcctg aatgtcaacg gcagcgaact cgtgaaagga
   129121 ctcaatgaca cgctgggaga ggccgtggtt gtcaccggcg ggctggccgg cgatgggact
   129181 cacttcaaac ggacatgggt gttgaaagat cgcacgcccc aaagcggcta cgtgaccgcc
   129241 gtcggattct atggcgacca catccggctt ggacatggct cgaagggggg ctgggataaa
   129301 ttcggcccgg aacgccaggt caccaaatcg atcggcaacg tgctctatga gctggacggc
   129361 cgcgccgcac tggggctcta caaagaatat ctgggggatc gtgcctccgg actgcccgcc
   129421 accgggctcc tgttccctct tgccatcaga acctcgcagg cagaggggaa agtcctggtc
   129481 cgcaccattc tggccgtgga cgaagccacc caatccatga cgtttgccgg agacattccc
   129541 gaaggggtct tcgcgcagct gatgcgcgcg aatttcgatc ggctgattca gggcgcatcc
   129601 gaagccgcga cgctcacgtt cgatcaccac aacggttcac acgccgattc gccgacgctc
   129661 tccatcgcca tcagctgtgt cggccggcgc ctggtgcttg gtgagcggac cgaagaggaa
   129721 atcgaagcca cactggagat tctgccgaaa ggcagccggc aaatcggctt ctactcctat
   129781 ggcgaaatct cgccctacaa gagcggcgcc tgcgacctgc ataaccaaac catgacgctc
   129841 acgaccattg ccgaacacta atatccatgc atccactgct taaacgacaa ctgaagcgca
   129901 tcgggctgga cgaggccacc gtgcctacct cgctcgacgg ctggcaacag ctcctcgaac
   129961 gggtgagcca gagctatctg gaatccgatc aagggcgcga acttctggag cgctccattg
   130021 ccctgtcgtc gcgagaaatg caggagctga atgagcaact ccgccgcaca tcggaaagcc
   130081 agctcgccgg ggaacgggac aaattgcaga cggtcctccg ctccatcggc gacgggctct
   130141 gcgtggtcga tggacactgg aatatcgtgc tgttgaatcc cgagggcgaa cggctgtggg
   130201 gtcttactga aaaagaggtc gtcggtcgcc agctgcacga catgatttcc ttgtcctacg
   130261 gcacgaagct gacggaaccg atcttcaccc agattctgct ggatgatact gcccagggag
   130321 gctcgttccg gaccgatgac gggctgctga cctcgaccac cgggcgctcg tttccggtgt
   130381 cgtgtgtctt ggcaccaatc gtccaggaga accgctcggc tggagccgtg ctcgtgtttc
   130441 gcgacattac ggaacgtaaa cagaccgagg acatccgtcg tcacagggag agtttgctgc
   130501 ggcggcaaca gaccactctg cttgagctga cgcgcaacag catcattcag agcggtgtcc
   130561 tggaaccggc gttgcaggaa attacccgcg cgacggcgat gaccctcgga gtgagccgga
   130621 ccagcatctg gctgttgagt gaagaccacg gctcaatccg atgcaaagat ctctacgaat
   130681 cgtcgaagca cagccactcc tcgggaattg agctcaagtc ggccaacttt cctgaatact
   130741 tccgtgaatt gctttccgag cgcgtgatcg atgccgcaga cgccagaagc gaccctcgca
   130801 cacgtgagtt caccgcctgc tatctcgagc ccctcgggat cggcgccatg ctggatattc
   130861 cgattcgctt caacggaaaa ctcgtggggg tgctctgcaa tgagcatatc ggcccgcctc
   130921 gcccctggat gctggaggaa cagcagttcg gccatgcgat cgcctccctc gtcgccctgg
   130981 ccctcgaagc cgtcgaacgg cttcacgcag aaggagcatt gcgcaagagc gaaggccgga
   131041 ccagactgat catcgacact gcactgagcg cggtgatcgg gatggacgag cgaggcctta
   131101 tcatcggctg gaatacgcag gccgagcaga tcttcggctg gacccgccag gaggccctcg
   131161 gccgtgcgat gacagacacc atcattccgc atgcgcaccg cgaggcccac caacgcgggc
   131221 ttgagcggtt cctcaagacc ggtgacggcc cgatcttgaa caaacgaatc gagattacgg
   131281 cgctgcgacg cgacggcaca gagttcccgg tcgagctggc cattaccccc ctccgtatcg
   131341 agaatgccta taccttcacc gccttcgtgg tggatatttc agaacgaaaa gaggcggaag
   131401 aggccctgcg cacgagcgaa gcacggttga cgatgaccgt gcagggctcg cacatcggga
   131461 tctgggactg gaacctcacc acaggcgtga tctatttctc tccgcaatgg aagaaccagc
   131521 tcggctacga cgacatcacg ctccccaata acttcgacga gtggcggacg cggatccatc
   131581 ctgacgacca accattcgtg caggaaacca tccagacctg tctggatggc caacgatccc
   131641 agttcgaact tgaacatcgt cttcgacatc gagacggctc ttatcgatgg atcctgtccc
   131701 gcggcagtct gattcgagat gtttatggcg taaccgcgcg catggtcggc atccacatcg
   131761 ataccaccga ccgtaaacag gctgagcagg agctccgtgc cgcgaaggaa gccgcagagg
   131821 ccgccagcaa agcgaagagc gagtttctgg ccaatatgag tcacgaaatc cgcactccca
   131881 tgaacggagt attgggcaca accgaactgc tgctcaacag tgcgctgacg gacaaacagc
   131941 gacatctcgc gtcgaccgtg catcgttccg gaagaacgct gctggccatc atcaacgaca
   132001 ttctggactt ttcaaaaatc gaagccggga aactcgattt ggaatgcgtc gacttcgacc
   132061 tgcaaccggt gctcgaagaa tcgctcgagc tgttcggcga agcggcccgt cggaaacagc
   132121 ttcgcctttc tcaacacatc gacgacgcgg tcccgcgcta cctcaagggc gacccggtcc
   132181 ggttccgcca gatcctcatg aatctgctga gcaacgcgat gaaatttacc gaaacagggg
   132241 gcgtctcgtt gagcgccgag tatgtggaag gaaccaccac ccaggccctg ctgcgcttcg
   132301 ccgtgaccga caccggcatc ggcattccgc cggcggccaa gtcacgcatc ttcgacgcat
   132361 tctctcaggc ggacgggtcg accaccagac gatacggcgg gaccggtctc ggcctttcca
   132421 ttgccaagca gttggtcgga ctcatgggtg ggaccatcac cgtggaaagc acaccgggaa
   132481 gcggctccac cttcgcgttt acgacgcggt tggatctgca acccctgggg tcgggcctct
   132541 ccacgaagct cgcgccgtat atgcaatggg cagagcccct tgtctcgtcc ggacagacga
   132601 gcgacacccc atgcccacac gcatctgagg cgggagcagt agcgccgagc cccaaacaga
   132661 gcgggcgaat tctgttggcc gaagacagtc ctgtgaatcg cgaagttgcc gtcggcatgt
   132721 tggagcaact gggctatgaa gtcgaggtgg cggaaaacgg ccggcaggcc ctcgcggccg
   132781 ccgagcgcga ccgtttcgac atcgtgctca tggactgcca gatgcctgaa atggacgggc
   132841 tcacggccac cggagaaatt cgccgtcgcg aaacagcctc cggtcggcgt cggctcccca
   132901 tcatagcctt gacggcgcat gccatgcagg gggaccgtga acaatgcctc accgccggca
   132961 tggacgatta tctgactaaa ccattcaccc agatggagtt gcatggaatc gtccgcaaat
   133021 ggctgagtca ccatgagccc gcctcgcagg ccgtacagcc gaacgctgca gctcccggaa
   133081 ctcaatccgt gccaccccga gccacgactg agccggcagc ggacacccta tctgcatcgc
   133141 ccggcatgga cctcaaggcc ctggagggca tccgtgcgtt gcaacgtccc aaccggccga
   133201 acgtgctggc ctccgtgctt cgcaagtatc tcgacaattc gcgagaaagc gtggatgcac
   133261 tacgtgacgc catccgcgcc aacgacccga tggccctgga agcaatggcg catcgcctca
   133321 aatcaagcag cgcgcaactc ggcgccattg ccgtagccgc cagctgcaaa gaactcgagg
   133381 tcatggggaa ccgaaaacag ctcgtcgatg ccgaccgaat ctttgcccag ttggaatccg
   133441 actatcgtgc cgcttgtacc gtctttcgaa atgagatcgc caaggagaac cagacatgaa
   133501 cccgctgata ccaggacgag caccctatgc actgatttcg gacgacgata tcatcatccg
   133561 tatgttcgct cgggaggcac tggagcaagc cgggtgggtg gtcgaggagg ctcaaaacgg
   133621 aattgaagcc tgcgaaattt ttcagaagtg tccgccggac gtggtgctgc tggacgtcat
   133681 gatgcccggg atggacgggt tcgccacctg cgcggcatta cgccggctgc cgggtggcga
   133741 acataccccc attctcatca tgaccggact ggacgacttt gagtcgatta cgaaagccta
   133801 cgatgccggc gcgaccgatt ttatcgtcaa accactgaat gcgatgctgt tgacccacag
   133861 gattcgctat atggtgcgcg ccgcccaagt cctccaggaa ctcctggaga gtcaggccac
   133921 cctcacgcaa gctcgcgatg ccgcaattga aggcgcccga ctcaaatccg aatttctcgc
   133981 caccatgagt cacgaaattc gcaccccgat gaacggcgtc ctgggcatga cagattggtt
   134041 gctggacaca ccccttaccg ccgaacagct tgactgcgcc cagacgatcc gctcatcggg
   134101 cgatgccttg atggtcatca tcaacgacat tctggatttc tccaaaatcg aatccgggaa
   134161 actgtccctt gaactgctgg acttcgactt gcccacattt ctagaccgtg tcctggcact
   134221 ctttggagaa cgagcacaac ggaagggttt gttgctgact tcgcggatcg ccgaggacgt
   134281 gccaagcggc ctgtgtggtg acccggcgag actgcaacag atcctcagca atttgctcgc
   134341 gaacgccatc aagttcagtg agcacggatc cgtctccgtc ctggtggaac tcgatcagcg
   134401 accagccgaa caaggccttg agtttcctgc gaccaggtat ggcgaactgt cggcgcagca
   134461 ggagcgggtg acgcatatcc gattttccgt ctcggacaat ggaatcggaa ttgaacccag
   134521 cgccttttca aaattattcc agccgtttgt gcaggctgac gggtccacca ccaggaaata
   134581 tggcggaact gggttgggac tggccatctg caaacagctt gttgaattga tgggtgggca
   134641 tatcggagcg gaaagcgaac cagggtccgg ttccgtcttt cgattcaccg tgccgcttca
   134701 acaacaatta ccgaacagcc tctccactag acaggcggcc tgagcaccaa gcctcaaggc
   134761 tcccatagca cctcatttcc cctcagccta tcagcgcacc aatcatcacc tcatcaccac
   134821 gcaggagtgt atctattaag atactcgatg cgtgaattcg atgatacggt cgcgcgaagt
   134881 atgcccgcct ataattgcta tcagattgaa atgcttagct aaagtgcatg gcttaaactt
   134941 tgctttactt cgtcctgcgc acatagtata caaagcggtc gaaaagatct tcttggtagc
   135001 cttcttcagc atgcatatgg cagctcttac ttttctcgct tagtaggcaa aaccctacac
   135061 ccaaggatca caccagtgca ttgcctgaca ttcgacatcg aagaacactt tcaagtttcc
   135121 cgtttcgatt ccccaattcg tcgacggcac tggggatcat ttgaaagtcg agtcactcca
   135181 aacacctgca aggtgctgga tctcctcgcc cgctaccaaa ccagggcaac cttcttcgtc
   135241 ttgggatggg ttgcggagcg tcatcccggg ctcattaagc agatcgccga atgcggccat
   135301 gaaattgcct cacatgggta cggccatgag ctggtcacag cccagacgcc tgaattgttt
   135361 cgtgcggacg tgagaaaagc caaacagatc ctggaagacc ttacgggctc tccggtccag
   135421 ggctatcgcg cgccgggctt caccattacg cgtgaaacgc tgtgggcctt gccgattctg
   135481 gcggaggagg gttacaccta cgactccagc atcgtcccga tccggcacga ccattgcggc
   135541 ctgcccgggt ccgatccctg gcaccatctg agacagacct cttccggccc catttgggaa
   135601 atccccccat ccacggtgaa cctcggcgga gtgcgacttc ccattgccgg tggcagctac
   135661 ttccggctgc tccccttccc ggcgctctgc gggctgttgc gacggactga acaaaaaggt
   135721 cgtcccctgg tcatgtattt ccatccgtgg gaactcgatc ctctacagcc gcacatggaa
   135781 ggaccgctgc tctcgcaatt cctccactat ctcaacctcg acaagatgga gttccggatt
   135841 tctacattaa tgaatacgtt ccgattcggc cccatcgccg aacagatcga cctgtcgccg
   135901 atcatgcctc tcgatccgac gatcttcccg gctcttcctc ctgccgaaat cgcgcaagac
   135961 gtggcgtagt cacgtcgata cttcccacgc aggcattgcc cgacccaccg gcagcagtgc
   136021 accattgctc acgctgcgcc aaagtaggga ttgcagtgtg tatgcggttt cagtacggta
   136081 ggggtcgcct gaacgaatcc agcacaacgc cctcacggag actgaccatg catcacggga
   136141 gtaggaaccg gcgacagcag tcgcccaaca caacgggcct ggcatgggca ctagcctgca
   136201 ttattttatt acccgcagcg gccgccggac agagcctgga tgacaccgtg cagttcatca
   136261 gcgacatggc caatactcac ggctttgtca ggagcctgag ttgcagaaat ccgaaggcgg
   136321 gaaaaccgac gaaaattacc gagatctaca gtgtcattcc aaccggaacc aaactcggca
   136381 cgatcgaact caatcacgga catgatgagg tcaatacggg cttcacgcac ttcgatctgc
   136441 acgatatcga cacgatcgag tatcagggcc aagacacgaa agagaacaac ctggtcctgt
   136501 atggcgtccg catcacgtgc aagagcagcg gcccctgcat catgaagacc agtttctgta
   136561 ccggagccgt caccgaactc gaagcacagt tccctgaaga taccttgctg tttcgctccg
   136621 cctctcatgc cgagcgaatg accaaggcgc tcgctcactt cttagggttg gtccgcgccc
   136681 aacggcagaa ccaacccttc tgaccgatat cggtctgctc cgggccgaca ctcgtgaaga
   136741 gatcggcccg gacacaccgc tgtctcacct actcaataat cgtcacagtc atttcaaccc
   136801 gatcgatcgg gttgtcgcat ggccccttat ccgcaggact cggtgcgccg gtctggcaca
   136861 tgactcgcgg cagcgccacg atcttgtccg ccaccccgat gcctttgacg acctcgccga
   136921 agatcgtata tttacgatct aagaaccggg actcctcgac gacgacaaaa aactgggatc
   136981 ctgcgctgtc cggatcctgc gcccgcgcca tggataccac gccacgcttg tgagggagat
   137041 cgctaaactc ggctttgaca ttgtgccccg ggccgccctg gccatacgcc cccttcttca
   137101 gcgaatcctt tgtgttggga tcgcctccct ggatcatgaa tcccgggatc gcccggtgga
   137161 aaatcgtacc gttatagaac cccgacttgg ccaacttgat gaaattctcg acatgcttcg
   137221 gggcgacgtc cggaagaaac ttgatctcga tatcgccgaa cttcgtcttg atgaccgcac
   137281 gcgcgttact cgcactgaca gattcgcttc cggccggagc ggcatcggcg gccaggccga
   137341 ttcccgcgat cccgactgtc atgaacagac tcatcattcc cgcgattctt aaacgattgc
   137401 tccatcgctg ctgcgccatc accatgcctc cttctagtcg gtccgcgacc tcttgctcac
   137461 gccaactgcg tcaacgcttc cttcgccgcc atttgctctg cttctttctt actgcgacca
   137521 cgcccgatcc ctcgcatgac tccctgaatg gtcagctcga cctcaaagac tttctcatga
   137581 tcaggacccg attcccgaac cgtctcatac tgcggcaaac tttcataccg tttctgacag
   137641 acctcctgca gctgcgtttt gtaatcctcc attcccggcc gggcctgctc agcgcgcgtc
   137701 gccagcaatt cctcctccag cacctgcaag gtgaattttc tgctggcctc aagcccaccg
   137761 tccaggtaga tggcggcaat cagcgcttcg agcgcgtcgg ctaacagcga atgcttctcg
   137821 cgcccttttg aaagttcttc gcctttcccc agacgcaaca gccgacccag cttcatgcgc
   137881 ctggccgcct tcgcgagcga agcctcgctg accagatgcg ctttgagctt ggaaagtccg
   137941 ccctcgttgc tacccggaaa ctcggccgcg agatactcgc tcatgacaag cgagagcacc
   138001 gcgtcgccga gaaactccag gcgttcgttc tgggttcgat ctttgctgcg ccgttcgttt
   138061 gagtaggact tgtgagtcag agcttcttcc aacaggcgag gctgatggaa gcggtagccg
   138121 agaaggcgct ggaccgcctc aattgacgtt gccggcgtca tacgacctga ctcgcgccct
   138181 gaagtggatt actgagcaca ccggcgaaag agcaggcagg cgttcacccc gccaaaaccg
   138241 aaggagtttg aaaggaccac ctgcgtctgg acggaacgcg ctgtattggg cacgtagtca
   138301 agatcacagg cgggatcggg atgatcaagg ttgatcgtgg gaggcaaaat gccatggtgc
   138361 agcgccaaaa tgctaaacac cgcctcaata ccgccggccg cacccaagag gtgtcccgtc
   138421 atggatttcg tcgaactgac aggaatccgg taggcttgct ccccaaacac atgtttgatg
   138481 gccttggtct caatggcatc cgccatggta gatgtgccgt gggcgttgat ataaccgatg
   138541 tccgtcttgg ccacacccgc atccttcaat gccatttcca tacaacggac cgccccctcc
   138601 ccttcttcag gcggggcggt aatgtggtac gcgtcgctgt tcatggcgta cccgatcact
   138661 tcggcataga tgcgggcgcc acgcgcgcgg gcatgctcaa gttcttcaag caccaccacg
   138721 ccggcccctt cacccaatac gaacccatct cgatccttgt cgaacggacg gctggccttc
   138781 gtcgggtcgt catttctgaa agagagggct ttcgccgagg caaaccctgc cacgccgagc
   138841 ggcgtaatcg ccgcctcagc accgccggca atcatcacat ccgcatcgtc acgctgaatg
   138901 agccgatagg catcgccgat acagtgatta ccggtggcac aggccgtcac ggcgcaggag
   138961 ttcggccctt tggcgccgac tcgaatcgcc acctgcccgg aggcgaggtt gatgatcgtc
   139021 atcgggatga aaaacggaga gacccgcccg gggccctttt ccctgagtac gtcgtggtaa
   139081 tgttcgatcg accccagccc gccgataccg gacccgatgt agaccccgac cctcgtggct
   139141 tcttcaggcg ccaccttgag tttcgcatcg tccaccgcca gctgactcgc ccccaccgca
   139201 tagtggatga aggtgtccat cttcttgatt tcttttttct cgatgaactg agccgggtcg
   139261 aaatccttca cctctccggc aatctgcgca tcgtagccgg tcggatcgaa tcgcgtgatt
   139321 cggccgatcc ccgactctcc ggcgcacagc gctttccagg tcttctccac cccggttcca
   139381 agcggagtta ccagtccgag accggtcacc accacacgtc tggcaggtcg atcgtgcata
   139441 cctacggttc cgttatgcct tctctttaat ataatccagc gctttgccga cggtgaggat
   139501 cttttcggca tcctcatcgg ggatctcgat ctcgaactct tcttcgagag ccatcaccaa
   139561 ttcgaccgtg tcgagcgaat cggcgcccaa atcctcgacg aaatgggcct cgagcgtcac
   139621 ttcttcttcc tcgaccccca actgctcggc aataattttc ttcacccgtt catctactgt
   139681 tgccatggac ttgcccacct ccttccccat cttacttgac ctccacattc ctcaccggga
   139741 gattccggat cgaggacacc aacccggaca cccgacctga ctcacttata ccatgagcat
   139801 gccaccgttc acgtgcaaca cttgccccgt gatatacgac gctgcatccg acaccagaaa
   139861 ccgaaccgcc gccgcaatgt cggagggaag ccccaggcgt cccagcggaa tctgcttctg
   139921 caacgcttcc ttgacctccg gcgacagccc ctgggtcatc gccgtatcga taaagcccgg
   139981 cgccaccgca ttcaccgtca ccgcacggct cgcatactca cgcgcgaccg tcttggtaaa
   140041 cccgatcacc gccgctttgg acgccgcata attcgcctgt cccacattcc ccatcgcccc
   140101 gacgatcgag gcaatgttga cgatccgccc gtaccgttgt ttcgtcatcg ggagcaggac
   140161 cgctttcgtg caatgaaagg tcccgttcaa attgacctgc agaaccaggt tccaatcttc
   140221 ttctttcatt ctaagcaaca atccatcacg ggtaataccg gcgttgttga ctagaatatc
   140281 aatcttcccc cactcctgca tcacctgatc ggccatggcc ttggcatcat tccaatcggc
   140341 cacgttcacc ttcacactga gcgcccgtcg ccctaacttc tggatcgccg ccacggcctc
   140401 ctgggaacgc gccggatcga ggtcggcaac cgcaatgtct gccccatctt cggccaacgc
   140461 ttctgcaatg gcccgtccga tcccctgggc tccaccggtg acaatcgcca cttttccctg
   140521 taatgacatc agcctgctcc ccgcaatctc aactcgtctt acgcctgact gttcacgccc
   140581 agcattctga gcgtcgcctc cagcgacttc ggatcattga cgttggccgt gacagccccc
   140641 ggaagaatcc tcttcaccaa ccccgtcagc acggttcccg gcccgatctc gacgaaggtc
   140701 gtcacgccga gcttggccat cgtctgcacc gactcttccc accgcaccga tgacggcaac
   140761 tgccgaacca gtgaggcccg gatgtcttcg gatcgttgca gggccgttgc ttcagcatta
   140821 ttcaccaacg gcacggtcaa atcacgccag accactccac cgaactcccc tgcgagtcgg
   140881 tctgctgcct tctgcatgag cggcgtatgg accgggacac tgaccgggag tggaatcgcc
   140941 ttcttacatc ccttggtttt ggcaatctca atggcccgtt cgacggcggc cttttctccg
   141001 gcgatgacga cttggccggg agaattaaaa ttcgcggccg ccaccacacc gaccgaagcg
   141061 gcctcctgac agaccgcctt gaccacggcc gcagaaagcc ccagtagcgc ggcgacgagt
   141121 ccggttccag gcggaaccgc ttctgacata tagcgtcctc gcttctgcac gagggccacc
   141181 gcatctcgaa acgacacccc gccggccgca tagaccgccg aatattcacc gagactatgc
   141241 cctgccacag ccaccggctt caacccggcc ggctccaatg ccttgagcgc cgccgcgcta
   141301 ctcaccagca acgccggctg ggtattctcc gtcaaattca accgttcagc cggaccttca
   141361 aaacagagct gggcgacgtc gtatccaaga atcgaggagg cctcatcgta gaccgccttg
   141421 accgccggag acgcctcgta aaacccgcgc cccattccta cagactgcga cccctgtccg
   141481 gggaacaaaa acccaatttg tgacgccatc atagccgcgt aaggtatcgt agaaaccacg
   141541 ctcaatgtca acgggaaaaa gctgaacgag ccccgcgcgt cagtagcgac cgaccgaccc
   141601 cgaccaggcg tttcccgact accagcggac caatgcggag gcccaggtta gtcccgcccc
   141661 gaaggcgccg aacatcacca actgcccctc tttgacccgt ccatcccgca ccgcttcatc
   141721 cagggcgatg ggaatcgacg cggcggaggt gttgccgtat cgatccatat tcaacaccac
   141781 tttttccagc ggcagattca ggcggtcggc aaccgcttta atgatccgta agtttgcctg
   141841 atgggggata tagacatcga gttcatcgac cgacaggtga tgcgccgcaa gtgtctcccg
   141901 cgccacttcc tcaagagtcc gcaccgccac cttgaacgtt tcattgccct tcatcttaat
   141961 gtactgcgag cgctcgctca ccatggcctc tgacggggga atgcgcgttc caccaccggg
   142021 caccacgatc aaatcagaga ggcttccatc cgaatgcaag tgagtggaga gcaccccgcg
   142081 ctcaccttcc gatgcactca cgaccgccgc accagcgccg tccccgaaca gaatacaggt
   142141 gttgcgatcc gtccaatccg tgatcgtcga catcacttcc gaccctacga ccagaatatg
   142201 ccgcataccg gttcgaatat aggcatcggc cacaccaagc gcgtagacga atccgcagca
   142261 ggcggcagac acatcacaag ccgctgcgcg ggtcgctcct agccggtgct ggagcaggca
   142321 ggcagtggag ggtaacggca tatcccccgt gcaggtggcc aggaggatca tgtcgagttc
   142381 atcggcggaa atgcctgcgg ctttcaaggc gcgttccgcg gcaatcaggc ccaagtcgga
   142441 acaggcctgc ccctcggcgg caatgcgacg ttcgcgaatg cccgtgcgct cgcgaatcca
   142501 ctcatcggac gtcgcgacca gcttctccaa atcggcattg gtcatcaccc gttcaggggc
   142561 ataggacccg gttccggtga ttcgcgctcg tatcacgcct gcttcccttc cggtcgttcg
   142621 tcctggtgcc gtctcaggct ttcttcaatg tcacgctgga tcaattcgtc cagcctggtc
   142681 tcggctaagc ccttcgcgcg gcgaatcgca ttcttgatcg ctttggctga tgaacggcca
   142741 tggcagatca tagtggtccc attcactccg agcagaggcg cccccccgaa ctcggcataa
   142801 tcgatcttcc gtttcagatt cagcaacggc ttcgcaatca gcggataggc taatcgcccg
   142861 agccaggagc cggatatctc cttcatcaac aacttcttga tgacgtccgc aaccccttcg
   142921 gagatcttga gcgccacatt accgatgaat ccatcgcaga ccaccacatc ggcattgccg
   142981 ctgtacactt cccgtccctc gatgttgccg atgaaattca gcggactggc cttcagcagt
   143041 ttaaaggctt ctttggtgac ctcgttgccc ttgctgtctt cctcgccgat gctcaagagc
   143101 cctatgcgcg gattctgctt ggcgtacacg tgcttggcga actcgttccc catcagggcg
   143161 aattgctcaa gatgctgtgc cgtgcagtcg acattcgcgc cgacatcgag catcaccgcg
   143221 ctgcccgtca aggtgggcag catggtggcg atcgccgggc gctccacacc tttgattaat
   143281 ccgagcacaa aaaacgaggc aaccatgctc gcgccggtat tccccggact caccaccgca
   143341 tcggcttcac cggatttcac caactccgtc gccacccaga tggacgagtc acgcttcttc
   143401 cgtgccacgg tcgccggcga ctcgtgcatc tccaccacct gcggggcatg gtggatcgtg
   143461 atccgcgtat cggtgcaatt aagtttttga catgcggcgg tcagctcatc ctgcttgccg
   143521 accagaatga tcccgacacc cagttcttgc gcggcttgca tcgcgccttc aatgaccggg
   143581 gccggtccgt ggtctccgcc catcgcatcg acggcaatct tcatgtgcgg ttagcggtcc
   143641 acggatagag atgtgaagaa tcgtgtagag acgccttgcc ggctaggacc tgaacggcct
   143701 cacgcatgac atagcgtcgg atcgtgatca cggtgcgcaa ttgtacccga atcacgtctg
   143761 gaaaataaaa ctcggctagc tttcctcgac gacgatgacg gccttccctt tgtaggtccc
   143821 gcaattcatg cacgtatagt gcggcagctt taattcgtga cattgcgggc acagcgaaaa
   143881 cccaggggga accagcctgg tcttggccgt tcgccgcata tctcgccggg accgtgaatg
   143941 tttgtgtttt ggatttgcca tgacgactcc ttacaacggg tacatgcccg gttaacagac
   144001 tatgcgtttc cacccttgga taggcgcccc cgcagcacac gaaagggact cggcccttgc
   144061 tccggcgggc agccacagcg acgctcattg agattctggc cgcactgcgg acagagacca
   144121 aggcaatctt cccgacataa cggctgcatc ggagcagcca ggatcacctg ctcgcgcacc
   144181 attggcacca ggtcgaggtg ttcgccctga tacagataga cttcgtccgc ctcttccgcc
   144241 tcttcaacag gctcaaccgg ctgggcaccg cgtctcggcc ctctccggcc ctgttccgcc
   144301 ggagccggct tggtcgccag gcccgcctgc ggaagatact cagcatagag attcacaaag
   144361 agcggatcgg aatattccgt gaggcagcgg acacactgac ggatcgccgt cccttccaac
   144421 gtgccggtca ccgtgatcgg accgtcgtgt tggacaagtt ccagttgaat cgtaagcggc
   144481 ccctcacact tctcttccgg ctcatccaag ccgagttcag gccccgtggc cgtgcacgac
   144541 agagacagtc cttctggggc gatatcgaca atcgctggct tgagcgtatc catcagcacc
   144601 gtcatgacgg gaagagccgc ctccgaacag agcgggagac agactgcccg gctacttgat
   144661 ccaagactca ccaaaaccta tccctcgccc tatcgctctt cagcgaaact aaccaaggcg
   144721 atctggcgcg cccgctttac cgccaccgtc aacacgcgct ggtgctgcag gcaatttccg
   144781 gaaatgcggc gtgggacgat tcgtccgcgc tcagtcagaa aattccgcaa caggccgaca
   144841 tccttgaagt caatcggcgc cttatcgaca caaaaccggc acggcttgcg gcgctgaaaa
   144901 aaccgaccgc ctccgccccc gccattcccg cgatctcctc gttccatact cgcctccgtc
   144961 tgctacagct gttcgtcttc gtaagaaggt tcttcgtgta ggccgccgct ctcgcctcct
   145021 gcctcgcccc gcttcggtag gaaggtcacg ccttgggcca cgacttcatg tttgctgcgt
   145081 ttctgtccgt cttcggtctc ccagcgtcgc tgctggagcc ggccatcgat aatgacgccg
   145141 ttgcccttgc tgagatactg cccgcaatgt tccgcctgtt tcccgaacac cacgatgtcg
   145201 atgaaacaga cttcttcctt gagttcatcc ccctgcttaa accgccggct tgtcgccaac
   145261 ccgaagctgg caaccggcgt tccgctgggc gtataacgca actcgggatt gcgggtcagg
   145321 ttcccgacca gaatgacttt attgaacccc gccaacggtg gctccttgtg gtgcgaccac
   145381 gggggcacct ctgggcggag caggctcctg ctccaacttc acggtcagaa atttaatgat
   145441 cgaatcttca agccggtagg cgcgttcgag ctcagcgatg accgctccgg gccccttgaa
   145501 gtagaagtag acataggtgc ccttgcgctc gcgcttgatc tcgtaggcga gtttcttgcg
   145561 gccccaattc tcggcccgct cgagcgaggc tccgttcttg gtcacggtgc ccttcatttt
   145621 ttcaatcaac gcagtggttt cttcgtcgct taaggtcgga cgaatgataa acagggactc
   145681 gtagagttcc atgcagaatc ctcctgggca tcggccccca ttcacagatg ggagcgaggt
   145741 gtaggtacgc cggacaagcc ggaacgagct ggcgaaggta gcacagcttc aaagaatgtg
   145801 tcaaacaggc gggcggcttt tcctgcgctg ccgagaccgg cacgctccaa tgatcgagtg
   145861 atcgagggtc cacaccgcgt gacctgccgt cgcagaacgc accagcacag gaaccatgcg
   145921 gtggcttcgc gatgacaccc aggctcacaa ttttttcttg aactgcgggg gtgcgtgagg
   145981 cgggagtgac ggacgagggc caggcgtgcc ggatggagga cgcgctgcgc cgaagccgcc
   146041 atacccacca taccagccga acccgagcgg cccgtaggga ccgccataat acccgagcgg
   146101 atgtccccaa tagccgggac catatccaat cccatacccg ggcccgtatc cgggaccggg
   146161 ccagtaggaa taggactgac tgccggcata gccgggatcc accagaaaat cactctgtgc
   146221 caaccgctcc gcctgatgga ctgattgcgc acaacccgcc aggagcagga tgacgcagag
   146281 cacgatgaac ccgggcacga atcgagtcat ggctgtgtcc tcaacgactg accacgtcac
   146341 gctccttcat tcagacacag acgctcgcat cgaatcaata tttcttctga aattgcggcg
   146401 gcgcattggc cggtggtgtc ggacgtggtg cgggtgcgtt cgactgctga ctgccgatgc
   146461 catagccgcc cccataccac ccatagccgg aaccatagta gccgaacgga ttccaccaga
   146521 atccagggcc gttccaatag gacggtgatt cgggataacc gggattcatg tgggcgctca
   146581 cagacccaag acgctctccc tggtgaaacg attgcgcaca ggccgtcagg agcaacagag
   146641 cgcttgcgac gaagagggaa ccgatcgatc gcgtcatggc tgcacctcct aaaaatgata
   146701 ggacaggccc acaaagagtt gatgtgcacg ataggtcgca tcaaagccgc cgaccggtcc
   146761 gaacgccccg ccgaaacgaa acgtagagtc cgtatatttg tattcggtga acaacgccac
   146821 cttcggcgtg acgaatgctc gaattccgac gaggacattc ccgccgaccg acacttgcgt
   146881 atcactctcc gtggtcgagg agcggccgag tcgcgcgaca agcaacgccg gccccccgcc
   146941 gatatagggc tgccaggtca cgccgggata gcgcaacacc acatgggcac cgatgttggt
   147001 taaggagaga tgggtccccg gcacatcgtc gaggttcttg atatgcgggg tactgtgcga
   147061 gacgtccagc tcaatcccga acaatccatg gccgggaaag acacccagct tgcctccgaa
   147121 ggcagggctt gtttttagat tgaaattcgg agcatccaga ccagccaggg tcccgctccc
   147181 tcggacattc cggaggggat cggcaaagtt tgccccaagc tgcgcggcca cataccattc
   147241 agcgcggatc ggtgagggag agcacaatag aagcacgcag cccaacaacc cggcactcaa
   147301 cctgctgcac acaaacctag tccacaccat ggtgctgact ccatgggcga ggcctatctg
   147361 gatcttatca ggcaatctgc gcggcagact ggacaacttt ggtgtgagcg aacgagggat
   147421 gtcgaatgcg tcacggacgg aacgctattc gctcgggtgc tgggatgttg agagagttat
   147481 ttcgatctcc tgctacggct cattgcgggc ggaggaatta tagagcaggt acaatgcgat
   147541 cccgccggcc acggcaatgc ccacccccgc cagagcacgt gtaccgaaac tcagcggccc
   147601 cacgacaaac tccgctgcac gaggtaatgc caccgtcgcg ataatgacca gcagtaacgc
   147661 aagcagatga atccagaggt agatgggttc gccgggtttt cgcataaatc agcgtgacgc
   147721 ttcacgcagt gtacgaactc tggccccatt attccactgt agaacaaggg gcgtcaggag
   147781 cgcaggcctg aggctcaatc gagagtgaac cgcttcgttt cttcgaggtg tttgctcaaa
   147841 tatggcatga agggcgtgcc gccggtgccg acagcggtgg gattgctggc gtgcgactgg
   147901 tgttgctgaa agatataccg ctcggcgtac ccgatgtgaa tgtctcgaaa ctgcgcgagt
   147961 aaatccacac aggtacaaaa ggctttccat agatcaggat gcgtcgcttt gcgatcgcgc
   148021 gcatagccgt agagcaacgg gcgatgctgc tcatccgtgc gtgcttcgag cgccgccaga
   148081 aacgtccggt ggcgcggagg catataatca cgcatctcca cgaggtactg cgtcagcgga
   148141 tcgtccgcat gagggattcc aaggccggca tccagcgccg gcacaatcga gctttgcgag
   148201 ccggtttccc cgcggaattg ttgaggtcgc tgcgcgtagt ccgcgacccc ttcgtagacg
   148261 actccctgag gcaatgacgg gctgttcttc cagccatgga tatagggccg cacgcggctg
   148321 taatagatat acggatcaca acgctcgggc atgcgaagca gcgtctcccg catcgcttcc
   148381 tgcgcggctg ccagcgcatg gagtccttgc agcacatcgt ccggcatatt gtctcgcgcg
   148441 gcgttgtgag cttgaaccaa tcccaccaac cccggtcctg ccttcgcctc aatatcgata
   148501 tggatcagga caaaccattc ttcatccagg cccccaagaa agttctgcag gagcaccacg
   148561 ttgcccaact cgatgggctt ctgcggatcg aggcgccgcc agttgtgcag ggcataggag
   148621 gcataggaca gcacgggcgg ccggccgagc tgttgcgcaa cggcacacca cggcagggcc
   148681 agactggccg ggatgagtga cgcggggtgg tcgggatctt cccagacaaa ggcatgaccg
   148741 gcaaaggaga gggtgcgcat cgcacaacga aagacatcga gaccccagtc atctgccacc
   148801 ggccccatga cctcttgatg gtcttggacg aaagatcgaa attgtcgcgc cgtcaggaat
   148861 ttcggaagct ccgcgccgat ctgattcaag atcggctcat ccgggagctg cgccaacggg
   148921 tctgtcgccg gaaggaaccc gcggtcaggt gaaagatcga actgcgcaag tgagagagac
   148981 tggtggctag acgtcgtcat ggtcgcttcc acgggagacg cacacaattc actctaccac
   149041 agtgcggcca tttccagcgc agcaccgcac tgcacccggc tcgcgtgaat ccaccggggg
   149101 actgaaaggc agttcttgac aagagctcta actcgggcgc tcgccaccct cggctttttc
   149161 ccgcacgttg aattgattca tcgccgtggc gacatcgcgg ttgaggagca attcgagggc
   149221 atcgacggct cgctccaggc agggggcata gatcgcccgc tcttcaggtg aaacctgttc
   149281 cagcacgtag tcggccgtat cctgcctcgg tgctggacgg ccgacgccga tcttcagccg
   149341 cacgaactgc ggcgttccca atgcttcaat gacagacttg atgccattgt ggccgccgct
   149401 gccgccgtct tgcctgatcc gcacacgacc cggttctaaa tccagatcgt cgtgcacgac
   149461 caccagggct tccggggtca gaccaagctc gcggaggagc cccttgaggg gagggccact
   149521 gagattcatc cagtcgaggg tactggcgag ttcgacgagt ttggaaccgg cgcgacccga
   149581 gcctcgctgg gccgcgcctt tcctggtgag acgtatcgac caccgcgctg cggcccgttc
   149641 gatcacccaa caaccgatat tatgccgtgt gtcggcatag tcggctccgg gattgcccaa
   149701 tccaacgagc aggcgcaacg caactacttc ttcttttccg cttccttctt cgcaggcgct
   149761 gctccggcct tgggcgcggc agccccagcc ttcgcgtctc ccgccttgcc ggcctccgca
   149821 ccggccgccc cttcagccac cgccttgccc ttcgccgcca cttcaggctc cttggcccct
   149881 tcagcaccag gcgccccggc cgtgagcaaa gcttcgagct tggcatcggt catcggcgcg
   149941 gccacactca cgaccatgtg ctcaaggtcg tccaaaaacc gcaacccctc ggtcttcgga
   150001 atgtccttca gatggatgcc ctggttgatt tggagctgcg atgcgtcgac ttcgatgaaa
   150061 tcgggcagga cagacgggag acattcgatg tgcagctgac gcaacacatg atgcaacacc
   150121 ccgccttctt tcagcccaag cggctgaccg ccggtcaagt gcaccggaac tttgacgcga
   150181 atcggcttat ccatcgccac ttcaaagaaa tccgcgtgca ggacgctgcc cgtaatcgga
   150241 tcgacctgga attcccgcag caacgcggta cgcttggcct tgctcttcgc gccgttgatg
   150301 gcgagggaaa tgagcgcggt ggatccggct tgagagcgca gaattttcac caactcatcg
   150361 ggcttgactg agagcaggag gcattccccc tgcccgtaca acacggccgg gacccgacct
   150421 gcacgacgaa gttgacgcgc gggccccttg cccccaccct ctctgctttc aacggttaaa
   150481 tcgaatttca tgatactcct cctcgaccag tcgctcgacg cggacggcct acacgaataa
   150541 cgaacttact gattcatctt catggatacg tcgaatagct tccccgaaga gaggcgccac
   150601 cgacaacacc ttgagtttcg gacacttctg ttccttgcca cgcaggggaa tcgaatcggt
   150661 caccagcact tccgtcagac cggactgttg gagccgctcc aatgcgggcc ccgataacac
   150721 gccatgtgaa caaccggccc acacctcacg cgcgccttta tccaggcagg cctgagcgcc
   150781 ctggacgatc gttcctgcgg tatcgatcat atcgtcgagg agcaacgcgc tcttgccctg
   150841 cacgtcaccg atgatattca tgacctgcgc ctggttgggt ccctcgcgcc gcttgtcgat
   150901 aatggccaga ttggcctgca agcgcttcgc gaaggctctg gcgcgctcca cgccgccggc
   150961 atccggcgac acaacaacca gatccgacac cttccgtttc gtgatgtagt ccatcagcac
   151021 cggcaaagcg tagaggttat cgactggaat attgaaaaac ccctgaatct ggctcgcatg
   151081 gagatccatg gtcagcacac ggtccgcacc ggccgtggta atgatgtcgg cgaccagttt
   151141 cgctgagatc ggcacacgcg gctgatcctt gcggtcctgc cgggcatacc cgaaataggg
   151201 aatcaccgcc gtgatacggt tggccgacga gcgctttaag gcatcgatca tgatgagcat
   151261 ttccatgatc gaatcgttga ccggggaaca gcaggattgg gtgacgaaga cgtcggcgcc
   151321 acgcacgttc tcttccacgc gaatccggat ttccccgtcg ctgaaggaag acaccgtcgc
   151381 cgcgccaagg ggcaggtcga gatacgagca aatcgcccgc gcaagcgcag gattggcgct
   151441 gcccgaaaag agcttgagtt ccctgttcat ccgacccctt cgatgccgta cgaggcgatg
   151501 cctatgctgt aacggtgctt gtcgagttcg taaaaaacgg tgggcacgga caggacccgc
   151561 tgtgagtccc gctgacctcg cgcacttgtc aggacaagag gacgcctacc gaagcacagc
   151621 acaatagcga aaacgccggg aagttgtcaa ccaatctgcg gcaggcgcag cgtccaccct
   151681 gcgatgcggg tgaggcgctc agccaccttt tgatagatcg tggccttgcc ccctctggcc
   151741 ttgcccttga ggactacggt ctcaagactc agaccatgct cgtgaccgga gtacccgccg
   151801 ccggtacggc ataggccttc atcttcgggt caagggcaaa cacagaggca gcccgctccg
   151861 catcggcctg cccgggaaat accccgaaca tcgtagcacc gctcccggac aacagggcca
   151921 cttcagctcc ctgactcagc aattggctct taatctgggc caataccgga tgctgcgaaa
   151981 aaaccggcgc ctcaaaatca ttctctacca ggggaatgat ctcggaccaa tccactgcag
   152041 cacggctgcc cagttgctgc aatgccggag agagaggacg cacccccacc ctggtggcag
   152101 acagctgctg ataggcccac tttgtttcga caggaaagcc gggattcacc agcacgatcc
   152161 agcgagtgcc cgtcatctgc attgatcgca cctgttcacc tcgaccggtg acgcaggcgg
   152221 cgggccccat aaaaaagaac ggcacgtcac ttcccagctg ttgtcccaac tccgccatct
   152281 gctcaatcgg ccagttcaat cccaacaggc gggtgagacc gaggatggtc gctgccgcgt
   152341 cgctgctgcc gccccccaag ccggccccca gcgggatgcg tttggtcagg ctgatcgaca
   152401 gatcgacggg tcgatcgatt cgcgccaaca cgagctgcgc ggctcgatag acaagattgc
   152461 tctgatcggc cgccagtgcg acatggtcgc agcgcaaggc gatacgggag gtcgtgccgg
   152521 gctggacggt aagagtcagt tcgtcttgga gtccgaccgt gtgcatcagc gaccagatgg
   152581 cgtgaaagcc gtcaggacgc cgttcaagca cgcggaggat cagattcact ttcgcagggg
   152641 tcagaacacg caggctggta gagttcactc gcagattact gggcggcgct aatcccggcc
   152701 tcctttgatt tcgtattttt cgagacggta ccggaacgac cgggtattca accgcaacaa
   152761 ccgcgcggct cgcttcttga cccactgact ccgttcgagc gctttcaaca gtagatcctt
   152821 ctcgatcgtg ttgatgagcc cttccaggtc caggccatcc tcaggcaact cagagggcag
   152881 ggcctgctgg ttgctcaccg gcttgtggag ccatccgcga atatccgcat ccgtcaccga
   152941 cgatcccgtt gtgaaagcca cgacacgttc gacgacgttc tccaattccc gaacgttgcc
   153001 tcgccactca tgcgccaaca gcacgcgcat ggcctcctgg ctcatcgtcg gcactggttt
   153061 accactttcc ttggcaaatt tctccaggaa atgttggctt aataacggaa tgtcgccggt
   153121 ccgcatgcgc agcgggggaa gcttgatcgg gattacatcc agccggtagt acagatcttc
   153181 ccggaaggcg ccctctgcga cggctttctc caaatcacgg ttcgtggccg cgacgatgcg
   153241 gacatccacc ttcacatcct gggttccgcc cacccgccgg aactcacgct cctgaatcac
   153301 ccgcagcagc ttcacctgga tggccggtgt cgtatcgccg atctcatcga ggaagatcgt
   153361 cccgccgttc gccacctcga acagaccggc tttattggag atcgccccgg tgaacgaacc
   153421 cttcatgtgg ccgaacaatt cactttcaag cagagtttcc ggcaccgcgc tgcagttcac
   153481 cgtcacgaac ggcatggagg cgcgcgcact gttgaagtgg atcgcccgag cgaccaactc
   153541 tttgccggtc ccgctttccc cgccgatcaa cacattgctt ttcgaatcgg ccaccttctt
   153601 gatgacgtca tagaccttct gcatcgcctc gctctgaccg atgatccgag agaacgacga
   153661 ctggctggcc agctcccgct tcaggagcat attctccgtc gataatcggc gccgctcgat
   153721 cgcgttgcgg ataatcagtt gcacttcatc gacctggaac ggcttggtga gatagtcgta
   153781 ggcgccgtgc ttcatcgcct cgaccgccga gtcggcggac gcaaaggccg tgatcatcag
   153841 caccacggtg tccggagacg cagccttgac ggccttcagc acctcgagcc cgcccactct
   153901 gggcatcttg aggtcggtga tcacgagatc gaaaatttcc ttctgcactt gcgcgatcgc
   153961 ttcatccccg tccgaggcca cggtcacggc atatccggcg cgcttgagca tgatgctcaa
   154021 cacatcgcgc aagccctgct catcatcgac gactaaaatc ttttccacgg ttcccttcct
   154081 tcatgccgga gccggacacc cgtctctgcg gactgcggga gacacacgac gaaccgtgcc
   154141 ccgtgcccga cctcgctctc gaccttgatc cacccctcgt gcaactccac aatgcgatga
   154201 acctgggcca gccccaaccc ggagccttct tttttcgtcg taaaaaacgg caaaaagatc
   154261 ttgtcgaaat tctgtttggg aatgccttcc ccgttatcct ggaatgcgat ttcgatgacg
   154321 tcggatcggc ggccaccgac ctccacccgc cgcactccgg tggcgatcgt cagcgtccct
   154381 ccggcggaca tcgcatcgaa ggcgttggtg gctaaattcc agaagacctg cttcagctgg
   154441 tcttgatcga cctgtgccat gagttggttc ggcgccatgc gggagacgat ggtgatgccg
   154501 cttcgagacc gcgcttcatg ttggatcaga tccaatgtat cggcaaggac tttgttcaaa
   154561 tcgtgctcgg ccaaattcaa cgcggggggg cgggcatatt gcagaaactc ggtgatgata
   154621 ttgtcgagcc gcctggcttc ccgaatcgcg atatccatca agcgctgact ggtgtcgtct
   154681 gacccgacat cctgccgcag catctgcatc gcccccgcaa gcgcgccgag cggattgcgg
   154741 atttcatgcg ccatgcccgc cgacatttca ccgagattgg ccagccattc ccgccgacgc
   154801 atctcctgct ccaagtagcg gagttgcgtt agatccttga atacacagac caggccgcgc
   154861 tgtgtgccct gttcatgtag cggagatacc gtcattccaa gtaccaagcg gctgccgtcc
   154921 gcatgcgaac attccacttc aaatcgcaag ggagagaggg acgtcaaggc ggcttcctcg
   154981 gagggggtac cggggtgcca attgaagaca tcccgccaga ggcgcccctg cacgtcagca
   155041 aaggcatagc cggtgacttc atgcgccgcg gggttgaagg acgtgatccg cccctcctca
   155101 tcggtcgtga acaccccgct gctgatactt cgcacgatac tctcgtgaaa cacctgcagc
   155161 cggttgagcc cctgttcctt ctcgcgcaac gactgatccg catgacgaag ctggtccgcc
   155221 aacactccgc ttaaaaatcc caccaccaga aatgccaacc cgtagacccc gaaggtttgc
   155281 agtgtctcgg cgccttccag ccggctcgca ggcagccagc cggaggcatg cagcagaccg
   155341 aaatattgga ccgccgtgac cagaccgaag aggagcgtac aaccggcgcc ggtgaccagc
   155401 cccacgcgtc gatgcgggac gagactggcg acggtcaccg taatgacata gagcaccgca
   155461 aagggacttt cgatcccccc ggtcctcgcg accaggacag tctccaggac aaaatccagc
   155521 cccacctgca cccatgtaaa cgccgtgagc gcagcagggg cgctcagccg tctcagtaag
   155581 agcgcgtaga cgatggtggt ggcataggtg aggatgatca gggcggagaa ggtcggcacc
   155641 agttcgccgc gagtggactg aaacgccagg gacagcccca gcatcagcgt caccacgacc
   155701 acacgaagcc ccatgagcca atggagtctg gtcttcagct caggcatcga gcgctcgccc
   155761 ggcgacgtcc ggtacggatc tgccagtggc gatgtctggg aggtcgagct ggaggacggc
   155821 tcagcaccgg tggttcgaac tggaagggtc gaggacagca tggggggcgc gttgccctga
   155881 tggatgtgca gcgcatggcc cgtttcgaag atcggaccat gcgccggaga acggacgcga
   155941 cggtgcagcg tcgctattgg atggcctgcg ccatcgtgaa gatcggaaga tacatcgcaa
   156001 tgacgataaa tccgatgatg gtacccaaca ccaccatcat gatcggttcg agcagcgagg
   156061 tcagcgtctc gaccgcctga tcgacttcgt cctcataaaa gtcggcgatc ttgcccagca
   156121 tggcatcgag cgcgccggtg gactcgccga ccgcgatcat gtgcgtgacc atcttgggaa
   156181 acacgttaca cttggccaac ggttcggaga tcgtttttcc gccgctgatg ctcacccgcg
   156241 cattcatcag cgcttcctcg atcaccttgt tgccggaggt cttggcgcaa atgctcaacc
   156301 cctcgagcaa cggcacaccg ctggtgatca aggttcccag ggtgcgggta aacttcgcga
   156361 cggaggcttt tctgatcaga tctccgacaa tcggaacctt gagcaacagc cggtcgatca
   156421 ccacacgccc gttcacggtg gcatagtacc gcttgatcgc aatggccgcg gccaccatgg
   156481 ccccgcccat ggcataccag tagctctgga aaaaattact cacgttgatc acgatctggg
   156541 tcggaccagg caatccgact tttccgccgg acatttccag aaacatctgg gcaaacacgg
   156601 gaatgaccca caccatcaag acactgatga tgatgacggc caccccgacg atcgcggtgg
   156661 gatagaccat ggccgacttg atctgcccct tcagcttcat cgccttttcg atgtgcttgg
   156721 ccagacgagt cagaatcgta tcgagcagac cgccgacctc accggcatgg atcatattca
   156781 catagaggtc gtcgaacact ttcgggtgtc gcttcaacgc atcggagaat gtggatcctg
   156841 cttccacgct gtttttcaca tcgccgaccg tttctcgcag cactttattt tcggactggg
   156901 tggagagaat atcgagacat tggatcaggg gcaatccggc attgatcatt gtgccgaatt
   156961 gacgggtgaa gaccacgaga tccttgtcgg tcaggccgct ccccaggcta aacttgaact
   157021 ttccgcccat gcccgacttt tcttccaggc tggtcacgac gacctgctgc ttgcgaagct
   157081 ggtccaccgc ctcatctcgg gtcttggcgg tgagctcccc cttcttcaca gcaccctggc
   157141 ggttccgtcc gacataggcg aatgtactca taacgcggtt tccactcctt tgaacaccag
   157201 gcaggatacg tacggatgca gcggaaaact atgatcgaag tctataagac gacctaaaaa
   157261 ctgtcaaacg aatgcacgaa agcggacccg cagaagaaca tgaaagaagc ggcacccaaa
   157321 gcggcggcgc gctatacctg gctggacgtg atgcggcaat agccgcggga gagatagtgc
   157381 aggccggaca tcagggtcac cccgcccatg agatacagga gcgggtcaag cgtggcaaga
   157441 tcaagacggc gagaggcaaa aaagataatg gccaccagcg tcgcgagctg aaccaaggtg
   157501 gtgcccttcc ccagtacagt cggcgaaata tcgacctggg tatgggtgaa atgggcaacg
   157561 gccgccccca gcatcaacat caaatcacgg ctgaccacca gaatcgtgag ccaaagcggg
   157621 acgagatgca tcacggagag cgtaataaac cccgtggtca gcatgagttt atccgccagg
   157681 ggatccagga cttctccgag tctggtccgc tgatcggcca cacgcgcgat gattccatca
   157741 agcgcatccg tcaatccggc aatgaagagc gtcgccagcg catagtcgaa ttgctcatag
   157801 ttgagcaacc ccacatacac cgggatcaac aggatgcgca gcatcgtcag actgttggga
   157861 acgttcatgt tcatcgaaac agctggtgtt tgaatcggcc cctcgactgg cgaacgatgt
   157921 cacgcgaatc gcgcatcata gaaacccctg gagaaccttg tcaacgacgg ttgcactccg
   157981 gaagggaggt ccctataatc gagacgcgta agacgctccc agcagatgat agacggaggt
   158041 ttgtatgagt ggactccccc tcctctttaa gaaagagggc ttaatcgaac ggcatcaagt
   158101 cgaggggatc gacccgagcg accgatattt taatcgagcg gtcctcgtca gtcgtgtggc
   158161 ggctggctat acggggaaag tcacctatga ggcctatgct gtcgaaggct ccgctcactc
   158221 caccacgggt gctgccgtca aggccgtcgt cgagaagttg atgggagtcg ggttcacccg
   158281 attgcgcact cgcctgaact tcaagggcaa ccgctacctg gccgaaaaag aaacctggac
   158341 cgactatccg gacctgccag cctaatcccc tagaaactcg cggcaaatca gatcgtgaat
   158401 gagcggacgg aatgttttga tccgttcatt tttcctcgtc ggatgtaccc ggttggacag
   158461 cagcactacc tccaattcct taaccggatc aatccatagc gaagtcccgg tatagccgag
   158521 atgcccgaat gattcgggcg tgaagcgcgt tccggaagaa gacggggctg acggcgtatc
   158581 ccaccccaaa gcccagctgg cattcggtat tccctgctga cgggtcgtaa acagggtgac
   158641 gaggtcaggc tgtagcaatc ccgtcttccc gcgccggcca tccatccacg ccttcgccac
   158701 cgcaagtacc gcgcgcgccg tgccgaacaa cccggcatgg ccggccacac cgcccagagc
   158761 aaaggcattc tcgtcgtgca cttcgccgca caacatgcgt cccctccaag gatcgtcttc
   158821 agtcggagcg atcgcatgtg attccaatga ggcggcaggc atggcctgcg actcgcgcgg
   158881 gacgtacgca agcggctgtg cctccagcgg gcgatagatc tggttgttgc aaaattggtc
   158941 cagcgactca cctgccagcc gctccaccgc ccatcccagc aacatgaacc ccaggtcgct
   159001 atacagactg cgagagcctc gttcatagac caacgcttct tgtgcgatat accccagcac
   159061 tgcctcgcgc gccgccgtac gtcccggaaa tccggactgt cctgcctcaa gcgaagccag
   159121 ccgctcgtaa taaggtcgcc acccgggaag ccctgagcta tgcgtgagca attgatgaat
   159181 agaggccgca cccgctgcac tgccctgcag cgcaatcaag atgcgatcga tccgatcatc
   159241 cagcgtcagc ttgccgcgct gcatcagcaa cagcaacgcc gtggtggtcg ccagcacctt
   159301 ggtcaaagag gccaggtcgt agcaggtgtg gatcgtcacc ccctcgcccg gaatctgcgg
   159361 ggacaggcgg ccgacagcac cttcatagat caacgttcct cgcaatcgga cagccaacac
   159421 cgcgccggga aaggcgccgt cgtcgacggc tgcctgtagg gcagcctgaa ggggatttgt
   159481 tgtggccatg aatgcgcgcc tacgtgtgat gcacagagag atggagggag aacggaaggg
   159541 cggagccccc ttggtagccc ccgctcgcag gcggagatgg aggcccaagg gagtgcagaa
   159601 tgctcaacgc gccgtgcttg attcgccggt taacttccct tcgaccgaag ccgcatccga
   159661 ctgatacgcg tcgctttctt ccacttccaa catacgttcg aacgatcgca gggttgaacg
   159721 gaatcgctcc accatcatca gacgctgttt ctggagatcc gcgagaccac gttgcatgtc
   159781 ggagagactc acccgggcct gccggatgat ctcactcgcc ttcagctccg cttccttcac
   159841 aatcaggtcg gactcgcgct gcgcagaccg tttcacatcc tcggccaacg tctgggctga
   159901 gaccaaggta ttcgagagcg tcgtctccgt gcgtcgcaag tcggtgacct gctgctcggt
   159961 cgcggtcagc ttttcacgaa gcgcactgtt ctctcgattc aagttctcga cggtcaatgc
   160021 cagctcctcg aggaaccggt tcacttcgtc ccggtcataa ccccggaact tgacctgaaa
   160081 gaccatctgc tgaatgtcga tgggagtgat tttcatacga cacctcttcc ccgacggtta
   160141 atgcatccgc acggcgatat cccgtaacga ttgaaccacg gcatattgca aaaagacaag
   160201 aatcagaatc gcgatcatcg gcgacagatc catgcccatg cgccatccta ccagccgacg
   160261 gatcggcgtg agcacgggct ccgtcgcgcg ctccaaaaac tgcacgatcg ggttccacgg
   160321 atccggattg acccacgaaa tcaacgcgcg ggcgatgatg acccacatat acagccacaa
   160381 caccgtatcc agcaccgtgg cagtcccctg cagcacattg ctcatcacaa acatcagcgt
   160441 cccaactcct gtgaacgttt tgtggccgcc tcgacggccg ccatcagaca gctccgcagc
   160501 ccaccggcct ccaattgata caacccggcg atcgtggtgc cgcccggcga cgccacttgg
   160561 tctttcaact tggccggatg ttcgccccgc tccaaaacca tccgcgcagc tcccaggacc
   160621 gtctgcgcgg ccaggagttc cgcggtcgct cgcggcaagc ccatccggac gccgccgtcg
   160681 gccaatgctt caatcgcgac gaatacatag gcgggtccgc ttccgctcag accggtcacc
   160741 gcatccatga gtcgttcctc gaccgacacg accgacccga ccgcctcaaa taatgtccgc
   160801 accgcagcca gatcgtcaga ttggagatca ggctgacagg tcaacgccgt cactccttct
   160861 cgcacgaggg ccggcgtgtt gggcatcgcc cgtacgattc ctctgggcgt cacgatccgc
   160921 tcgcgaatcc acgcggtcgt cacaccggcg gcgatggaga cgatgaattt tccggcgcag
   160981 gcctgccccg catcactgag caccgccggc atcgcctgtg gtttcaccgc gagaaccacc
   161041 agatcggcct cccggacagc cttcgcattc tcatcgccga cacgaatgcc gaagcgcgat
   161101 ttcaacgcat cgcgacgcgc ggcgaccgga tccgtcgcgc aaatggacgc aggctcacaa
   161161 gcccccgcgg ccagcagccc gccgatcagg gcctccgcca tttgcccgcc tccgaggaat
   161221 gcaatcggtt ttgtcagcat cgaactactc acctcgcgct ccgaaaatcg ccgttccgac
   161281 tcgcacgtac gtcgccccct cctcaatcgc aacgggatag tcgtgcgaca tgcccatcga
   161341 gagttcctgc atattaatgt tcctgtgccc acgcgctgtc aatgtctgag cgagggtccg
   161401 gagttgtcga aagtagggac gcgcgtcttc cgccgtcgga gtgggtgggg gaatggccat
   161461 gaggccgcga atatccagat gctcaagccc gttcagggat tccagggccg cccccagtgt
   161521 cgtcggctca aatcctccct tgctggcttc tccagcaaga ttcacttcca gcaggacacg
   161581 ttgccgcagc cccgcggtct tcgcctgccg gtcgatctct tccgccaggg cgagactatc
   161641 gacggaatga atcgtctcaa atcgcccgac gaccgatttc accttgcgcc gttggagggt
   161701 cccgataaag tgccagacga ccccttcacg ccctaacgtg tcgattttcg gcaaggcctc
   161761 ctggagacgg ttttctccaa gatgtcggat gccggcgtcc actgcctgac gcagtcgttc
   161821 aactgacacg gtcttggacg cggcgatgag acgaacagac tccggcgggc gccccgcacg
   161881 ggcggccgca cgctgcattt cgtcgagcac cgttcgaacc cggtccgcaa tcgttcccag
   161941 cgctgcatcc atcctgccgc cttgcggatt gctccacgtg tactatcacc cgatgccgcc
   162001 gtgaggacgc aataggacag gctccgggaa tgaagtccct aacgagtcga tctcaacccg
   162061 gccggcgagg caggagagca atgccactca ccatcgtcgc cttgaccacg ccttcgcgac
   162121 gataactata aaatcgctcg ggctgacaga tggtacaggc atcaaccgtg gcgacccgct
   162181 ctgcggccaa cccgtcagcc aacgcttgcc ggcgcacaaa ggcgcgcaga tccagatgcg
   162241 ccttctggct actcaccggt ttcacgacag atcgccactc gggaaagacc tcccgcagtc
   162301 gcgcaaggac cggctcatcc acttcgtaac agcagggacc agcggaagga ccgatcgcca
   162361 tctgcagccg atccacggtc gccccgaacc ggctcaccat caaggccacc gtcttcggca
   162421 caataccggc caccgcaccc cgccagcctg catggatcgc cgcaaccacc cgtcgctccg
   162481 gatcgtgcag gagcacaggc acgcaatccg cggtccgcac ggtcaccatc agccccggct
   162541 gatcggtcac caacgcgtcc cagcctcctt cgaaagcgtc gccctcctcg accggccgat
   162601 cgacgatcag cgcatccgtc ccgtgaacct gtttgacgga cagaatgacg ctcgcgcccc
   162661 gctcgcgatg cagggcccct ggcacagcgg cacgacccgg tgtcaccgag acggacgaca
   162721 accgggtgcc gaaaaaatgt tccaccccat ccgcaggcgt cgcaaacgac ggcgcggtga
   162781 tgacctctga caccatcccc gttccagacc tcccagtgac cgataccgct ccgtgctcag
   162841 tcggcctgct tccgtaggaa ggtcggcaca tcccattcgt catcgccgac caccgcgatc
   162901 cgctccacgg cttcccgggt ctcgcccatg cggcgaagga aggtcggacg atccaagtct
   162961 ttatgaggcc gatccgatcc cgcggcatgc acgccggtca gcacctgttg cgccgggcgt
   163021 ccattggggg tccgcgccgc gggacgttcg gccgtgacgg ccggtcgcgc agccggttgt
   163081 tcttcccgct cgaatccggt cgcaatcaca gtcacgatca gatcatcacc gatctcggga
   163141 ttgatgacct gtccgacgat gatgttcgct tcggcatccg ccgcatgctg aacgatcgac
   163201 gccgcttctt ccacttcgtg caacgacatg ttcggaccgc ctgtgatatt caacagcact
   163261 ccgcgggcgc cttcgacgct gccctcttcc aacaacgggc tgcagatcgc cttctgcgcc
   163321 gcttcctgcg cccggttggc gccacgaccg atccccatcc ccatgaccgc gcgacccgtg
   163381 tgcgccatga tggtccgcac atcggcgaaa tcgacattga ccagcccgat ggtagtaatc
   163441 acgtccgcaa tgccctggat cgcctgacgc agcacatcgt ccgccacctt gaacgcatcc
   163501 agcaacgggg tggccttgtc cacgattccc agcaaccgtt gattcgggat aatcaggagc
   163561 gtatcgacgt gccttccaag atcgcgaatg ccttcctcgg catggctcat ccgtcgatgt
   163621 ccttcatact ggaagggctt ggtcacgacc gcgacggtca ggatgcccag ttcacgcgca
   163681 atgctggcga caatcggagc cgcgcccgtc ccggtgccgc cgcccatgcc ggcggtgaca
   163741 aacaccatgt cggcgccgac cagactctcg cgaatctcat ctttactttc caacgcggcg
   163801 tcgcgtccca cttcgggctt ggcgccggcg ccgaggccgc gagtccgttc cggaccgatc
   163861 tgaatcttat acgacgcctg ggaccgctca agtgcctgca catccgtatt cgcggccacg
   163921 aaatcgaccc ggcacaatcc gccggtgatc atcgtattga cggcgttgca ccccgctcct
   163981 ccgacaccga tcactttgat gcgaacgggt gattgcggct cctcttgaaa tgaaaacatc
   164041 gtggcacctc ccttgacgat ggtgcggacg tggtcttaag ccgtccgcaa aagttagaaa
   164101 aactcgaaca tccacgatcg catgcgatcg aacactttcc ccaacccttt gccgtgacga
   164161 atgccggcag cctccaagtt gtcggcatgt tgacgtgcat gcaacaacag cccgacaccg
   164221 gtcgcgtgca tcggattgct gacgatatcg cgcagcccgc cgatgcccgt cggcatgccg
   164281 cgacgagcgg gcagattcag acctcgctcg gcggcatccg gcatcccttc caataatgag
   164341 gttccgcccg tgatcaccac gccggcaccg agcatgcctt catagccggc tcgcacgatt
   164401 tcgcgtttca ccagatcgaa catctcctcg acgcgcggtt cgagaatctc cgcgatatca
   164461 cggcgcgtga attgccgcgg cggccgatcg ccgaccgacg gcacttccac catttgatga
   164521 ccatgcacca gctcggtccg ggcaatccca tgttgcactt tgattttctc ggcgtcggtt
   164581 tgcgaggtga gcagaccgat agccagatcc ttggtcaaat tctggccgcc gatcgggagc
   164641 accgccgtgt gccggatgct gccgtcgagg aagatcgcta agtcggtcgt gccgccgcct
   164701 agatccacca tggcgacccc caactcccgc tcttcggcgc tcagaacggc ttcgcttgac
   164761 gcgaggggct gcaggataat gtccacgaca tcaagcccgg cccgattgac actcttgatg
   164821 atgttctgcg ccgacgtcac cgcaccggtg atgacatgaa cgttaacctc aagccggttg
   164881 ccggacatgc ccaacggctc gcgcacgccc tcctgatcgt ccaccatgaa ttcgcgcggc
   164941 aacacgtgca agatgcgacg ctcgtgagga atcacggcga gcgttcgcgc actttccacc
   165001 gcacggctga tgtcgtcgcg cgtgacttcc tgcttcttga gggccacgac gcctttgagg
   165061 ttttcccccg aaatatggct ccccgcgatg cccgtataga ccgaattgat ctgcacggcc
   165121 gccatgagct ccgcttcctc aaccgccttc ttgatcgact ccaccgtact ctcgatgttg
   165181 accaccacac ccttgcgaag cccgcgcgag gggcaggagc cgacgccgat gatattcagc
   165241 gggccctcct ccggcacttc ggcgacaatc gcacagatct tggtggtccc gatgtcgagt
   165301 ccgaccagga tatgatcccg cttagacact cggctcaccc ccttccccga acaatgacgc
   165361 ggtcggcaaa gcgaagatcg atttcattcg ccctggcgcc ctcgccgtca aaggccacat
   165421 cacgcagtgc gggccgcatt ttgagaaacc ggtaccattg ctgattcatc gacgactcac
   165481 tgaattgaaa cgtcacaccc tggacctcga ctaccagatt attgagattc cccacattga
   165541 tatccggccg tcctccggtc gtctgtccga ccatgcgggc cagggccgcg ccgacctgca
   165601 cggggcgacg atcgtccggt tttccctgca ccagccgctt gccgtcgaca ccggacaaca
   165661 tcggtagggt cggatcatcc gccgacccga gatgcgccag caagaacccg tccgaatcgg
   165721 ccaggaggtt ctccgccaag gtacgcacca ccaccgcagg ctcccgctcg acgatgtcga
   165781 tatgaatttc gtgcaagggc ttcagcacca ccgtcgcatc cttgatccag ggatgggtct
   165841 tgatccggtc ggccagccag gacgggttaa tggagtagag cgcggtatca ggcttcaatg
   165901 ccaatctccc gatgacttct ttcctcgtca catggtgcaa cccgctcacc gacacggagc
   165961 gcaccagaaa ccattcgcgc gtcagaggcc ccacttcccg tgccaggacg aacagcccag
   166021 aacacccgcc gaccagaacg gtcaccgtgg caaccacccg caaggtaatc agcaggcccc
   166081 gccccagcct ggaccagtac ccgcccgtct caccggcccg atggcggccc agcgcggatt
   166141 ccgaacgggc attcgcccgc gggttcactt tgaccgggcg taccttgcgc caccgcagcg
   166201 acatcgctac ctcacggctc gaaatgatcc gactccggcc cgcccgagcg cggactggag
   166261 aatccgctcc gtcaaggcgt cgtacgacaa tccggccttc cccgccgcca tcggcagcag
   166321 actcgtctcc gtcattcccg gcacggtgtt gatctcaagc acaaacggct tgcccttggg
   166381 cgtgatacgg aaatcgaccc gggctgcccc gctgcatccc aaggcctgat aggtccggat
   166441 cgccagttgc ttgatctgcc gagtgactgc ggccgggagc ggcgccgggc acaggtactg
   166501 ggttcgacct ttctcatact tggccgagaa gtcataaaat ccatccggcg ccacgatctc
   166561 cacggccggc aacccggtca ccaccccgtc ggggccgccc agaagcgaca ccgtgacctc
   166621 atgtccctgg ataaacgcct cgaccatcgc ttcttcgtca taccggtgcg ccgtacgaag
   166681 cgcctctttc cattgcgacg gcttccggac aatggtcact ccgatcgtgg atccctggct
   166741 cgccggcttg acgaccaccg gcaacttcaa cttacaggtg ctcagcactc gattcagact
   166801 aggagccgtg ccgcgaagca ccaccgtccc gcgcgggacc ggaatgccct gagcggccaa
   166861 ttccgtcttg gtcaccacct tatgcatccc aatggcactg gcccggacac cggaacccgt
   166921 atagggaatg ccgagggtct ccaaaaatcc ctgaatcgcg ccgtcttcgc ctcccggtcc
   166981 gtgcaacgcc agaaacccga cctcaaccgc ctgctcctgc agcgtcttgg acagatccgg
   167041 accgacatcg atggccaccg cgtcatagcc gcttcgcacc agcgagcgat acacagcctc
   167101 cccggtcttc agcgacactt cccgttcaga cgattgaccg cccatcaaga ccccgatttt
   167161 tgaacgtgtc agcggcttcc gttccgtcac atccactccc ttcatcgatc cgcgtcaggc
   167221 ctcaccgacc accttcagtt ccaattccag cttcacgccg gacacttttt tcacggccgc
   167281 ccgaaccttc ctgatcaacg ccagcacatc ggccgcgcgc gcatgaccca cgttcaccat
   167341 gaagttcgcg tgtttttccg agacctgcgc atctccgatg cgggccccct tcaggcccgc
   167401 cgcgtccacc aggcggcctg cggagtcctg cggagggttc ttaaacacac acccggcact
   167461 cggcaacgtg agcggctgag tattcttgcg atactgaaga tactccttca ctgtcttttc
   167521 aatccggtcg tgatcgccgg gcctcaactg cacccagact cccgccacga tgccgcgcgg
   167581 cagatgcgcc cgtcgataac tgaacggaat atccgcggcg ggaatatcca ccacccggcc
   167641 gcgaggatcc accatccgga cggccttgac ggactccttc atttcgccca agcgcgtgcc
   167701 tgcattcatc acgacgcacc ccgccacggt cccgggaatc ccagcgcccc attccaagcc
   167761 ggcgagcgac cgtcgaatgg catatccgat caacgtcggc atgcccacgc ccccttccgc
   167821 atagagtacg tggtccggtt cctggcggat ggctttgaat tgccgcaggc taaccacgat
   167881 cccgcggata cccccgtcac gaacgagcac attggtcccg cccaccacaa agaccggaat
   167941 gtgttccgcg cgagcctgtg ccaccactcg gcaaaggtcc tccacatcgg ccggctccac
   168001 cagcacttcc gccgggccac cgatgtggaa tgaagtatag gcctgcagcg aggcctcgtg
   168061 cgtgattgcg ccacgaaccc cttcaagaat acgcgcccag tctttcctgg ttcttgccgc
   168121 atgatctttg cccgccgcac gtgacacggt tgttcgcctc gtcgccccct aagccggcaa
   168181 tcgctcaaga atcgccaatc ccgttttcca gatatcccct gcgccgaggg taatcacgag
   168241 atccccggat ttcagggtcg gcagcacctg ctccgccagg gtgtccttgc gctccacgaa
   168301 ggtggccgaa ggatgcccgg ccgcctgcac cgcctccacc agcttcgcac cggacacgcc
   168361 agggatcggc tgttcgcccg ccgcataaat ttccgtcatg aagagcgcat ccgcctggtc
   168421 gaacgcatgg gcgaattcct gcatcaaatc gcgagaacgg ctgtagcgat gcggctggaa
   168481 cagcacgacg acgcgtcgat cccacccttg cttcgcggcg gcgatcgtgg cccgcacctc
   168541 ggtcggatga tgcccatagt cgtccaccac catgatgccg gccttctctc cgcgcagatg
   168601 gaagcgccgc tccaccccgc tgaacgccgc gaggcctttc cggatcagat cgaccggaat
   168661 atccaactcc atgccgatgg caatcgccac caaggaattc gacacattgt gaatgccggg
   168721 aatggacaac cggaacggac cgaggttgcg tccgcggaag aacacccgaa actcagaccc
   168781 ccattgccgc aggctgatat cggtggcgcg aaagtcggga acatggccgg ggtgttcgtg
   168841 cagaccgtag gtctgatagc gcttcactac gcgcggcagt agatttcgta accgttcatc
   168901 gtcggagcac aacacggcca ggccgtagaa cggcaccttg ttcacgaatt ccagaaaact
   168961 gtcgttcagc ctctccatgg tcccgtagtg gtccaggtgc tcacggtcga tattggtgac
   169021 ggcggcaatg gtcggtgcga ggcgcagaaa cgacccgtca ctttcatccg cttccgcaat
   169081 gagtagatca ccgcgcccca gccgtgcatg gctcccaagg gcattcacct ttccgccgat
   169141 caccatggtg gggtccaatc cgccctgtgc gaggacattg gccaccatcg aggtcgtcgt
   169201 cgtctttcca tgagcgccgg cgatcgccac gccgaacttg agtcgcatca gctccgcgag
   169261 catctccgca cgcgggatga ccgggatctg ccgcgctttc gccgccacca cttcagggtt
   169321 ggtcgccgcc accgcggaag agattacgac cacctgcgcg tcaccgatat tggactcatg
   169381 gtggccgatc gcgatgcgcc cccctagttc ctcaagccgg cgcgtcgttt ccgactgact
   169441 gaggtccgag ccgctgacct tatacccgag cgtcaacaac acctcagcga tgccgctcat
   169501 cccggatcca ccgatgccca ctaaatgaat atgttgtgtc tttctgaaca tcgccgtccg
   169561 ccttagctcc tgtcctcgaa atgatcgctg cccactgctc ctatcctgtt gctgtgtctc
   169621 ccgctctcgt tgttcatagg gcgcgagcgc tgtcgctggc ctcatggcgc ctccttatga
   169681 cgtcgtaaca ctcacgaacg atggtttcgg ctgcatcgct gcgtcgcata ttccaactct
   169741 gttcactcat ggtctgaagc cgatgcggct ccttcaaaat ctgtatgacg gtttgcgcca
   169801 gtccaggccc ggtcaattcc gcttgcggca ggagcactgc gccgcccgcc ttggccatga
   169861 cctcggcatt ccgcagttga tggttgtaga tcgccgtcgg cagcggaatc agaatcgccg
   169921 cctttccgca gacggtgagc tcggcaatcg tcatggctcc ggccctcgcg accacaagat
   169981 cggcgcctcg cagcaccgtg ggcatgtcgt agagaaaggg cacgacctgc gcgggcattc
   170041 cagcctgttc atacgcggcc acgactctgg cgtgatccgc ctcacccgtt tgatgggtga
   170101 tggtaagctg gtccttcatg gagcgaagca acggcaaggc ctcgatcacc gccgagttga
   170161 tggccttcgc accctgactg ccgccgaaga tcaacaggtg tcgagcggtg cgtgcctccg
   170221 cttgctccgc atccggagcg gcagattcca caaacgcctg ccgaactggt gtccccacta
   170281 cgctggtctt gcatcgatcg aaaaatgtca ccgtcgattc gaaggccagg aacacccgct
   170341 gcacgaacgg cgcaaccgcc ttgttcgcca tgccgggata ggcgttcggc tcgagaatca
   170401 caccggggat ccgccgtaga aacgccgcca agagcatcgc cggactggtg tacccgccga
   170461 cgccgaacac gagatcggca ctctgcttct tgaggatccc cagtgattgc cataaacttt
   170521 ttggcagggt gatcagggcc ttcaggatct ccagcagact cttgcccatc aaaggattgg
   170581 catcgatgca ctgcaaggga aaaccttcgt gggccagcac tttgcgttcg attccacgcg
   170641 tcgtgccgac aaacagaatc cgggtggatg gatcgcgctt caaaaactcg cgcgcgacgg
   170701 caatggccgg atacagatgg ccccccgttc cgcctgccgc aatcaccaca ttcattccgt
   170761 caccaccccg cgcttgcgca ccacccgcgg cccactgctg tcctgcccgc cctgccggtc
   170821 gcgtgaaatg ctcaacagaa tccccacgcc gaacaaattg gccatcaatg aggagccgcc
   170881 gtagctgaca aagggcaggg tcaagccttt ggtcgggagc agtccggtga ccactccggc
   170941 attgaccaag gcctgcatgc cgacgaggag cgtgatgccc attgccaggt gccgaccgaa
   171001 cgggttgcga gcccgcctcg cgatctgaaa gcccttgatg acgaacagac cgaacagcag
   171061 cacgatggtc acggtgccca tcagccccaa ttcttcgccg accagggcca gcacaaagtc
   171121 ggtatgcgct tccggcaaga aaaacaactt ctgtttccct tcgcccagcc ccacaccgaa
   171181 cgggccgccg ctgccgaacg ccagaaagga ttgatggatt tgatatccgg aaccggtcgg
   171241 atccttggcc ccatacagat attcgatcac tcgtcgacgc cgataaggag accccagaat
   171301 caaggccgcc accgtcggca gcgcacagag cgccaggagg cccaggtgtt tgattcgcgc
   171361 ccctgccagg aacagcatcg tcacgaccac caggcccatc accacgaccg ttcccagatc
   171421 gggctccagc aaaaccaggc cgctcagcaa ccccaacacg atcagcggcg gtaagaggcc
   171481 tcgagaaaaa ttggtgatct gatcctgctt cttggtcaga taggccgcga tgtatatggc
   171541 caccgcgtac ttcgccagct cggccggctg aatgttgagc ggaccgaggt gcagccatcg
   171601 ccgcgccccc tttgccacgc tgccgaacga cggaacgagc accaacacca gcagcaccgt
   171661 cgtcccgaac agcagcggaa tcgccaattt tttccatatg gtgtagtcga tcttcgaaat
   171721 gagatgcatc accaagaggc ctgcgcccaa ccaggccaac tggcgcttca ggaagtacca
   171781 ggggtcgtga aaccgattcc ccgcgaccac cgcgctggca ctgaacacca tgaccactcc
   171841 caccagggca agaatgagcg tcacggccag cagggccgga tcgaccggca cgcgtttggt
   171901 cgcccgctgg gtcgaggtcg accagggcaa catcagtgtt ccgagcgcat gctgtgccat
   171961 cgatcagctg tcgttcctcc cacacggctc ccgctttacg ccggcagccc ttgtaccagg
   172021 gccttaaact gccggccccg gtcttgataa tccgcgaaca tgtcgaaact ggcgcaggcc
   172081 ggcgagagta agaccacctc gccgggctgc gcctcgcgag cagcaagctc cacggcgtcg
   172141 cggagggtcg ctgcaggacg acaccggtca aaatctccca tcgcatcctg gatacggccg
   172201 gctgcctctc cgatcagaat cagccctttc acccgttccc gtatcgcgcc ttcgaggcgg
   172261 gaaaagtcgc cgcccttgtc tcggccgccg gcgattaacc aaatcggctg gtctattcct
   172321 tcaagcgcct tcaacaccgc atcgacattg gtgcccttgg agtcgttcac gaagcgaacc
   172381 ccgcgacgct cacgcaccac ctccaaggca tgttccagcc cggggaagga tcgcaacacg
   172441 gcgcgaatgg cctcgattga acaaccacag agcagcgcat acgtcaccgc cgccatcaca
   172501 ttttcgacgt tatggaggcc gatcagccgc atatcgctcc ggcggcagat ttcctcccgc
   172561 cgtccgcgaa ctgtcgtcac gatcaggtcg ccgtcaagca ccgtcgcccc cgccacaccc
   172621 gacaccgtgg cgccgccacg gctgaagccc aggacggtac ccttggtgcg cccgcgcagc
   172681 gcggccacgc gcgcatcatc gagattgaac agcgaatagt cgccggccgt ttggttggcg
   172741 aagatgttcg ccttggccgc cacatagtcc tccaccgagg catagcgatc catgtggtct
   172801 aaggtcacat tcaagatcga ggcaatccag ggatggaact gctccatcgt ctcaagttgg
   172861 aaactggaga cttccagcac cacatactca tagggtgcga ccgtccccgc cttcgcctgc
   172921 accgaggcca aggccgcctc gctggcggct atcccgagat tccccccgac gaaggcgcgc
   172981 tttccgctct cctgcagaaa cttgccgatc agcgtcaccg tcgtgctctt cccgttggtc
   173041 ccggtcacgg ccaccaccgg cgccgtcaaa aagcgggacg ccaattccag ctccccgatg
   173101 acccggcccc cccgggccct cacccgattg agcgcatcca gttgagtcgg cacacccggg
   173161 ctgatcacga caagatcagc cccttcgagt gcggactcgt actgcgcccc caccttcacc
   173221 gcgatgctcg accgttccac ctgcgacagg atggcggtca gttcctgcgc ctccttccgg
   173281 tcggccaggg tgacccgggc cccgagatgg ttcaacaggc gtgccgcacc cactccgctt
   173341 cgggctagtc cgacaaccgt gacttggaga tctttcacgt tcactgctgt tcgcctaccg
   173401 taatttgagc gtgctcaagc tgagcaacgc cagcaaaatg gcaatgatcc acagacgcac
   173461 cacgaccttc ggctcgtccc accccttcat ctcgaagtga tggtgaatgg gcgccatatt
   173521 gaagatgcgt ttcccccgga gcttatagga tcccacctgc aggatgaccg acaacgcttc
   173581 gatgacgaat acgccgccga ccaggagcaa cagcagttcg tgcttgctga tcacggccac
   173641 agtaccgagg gctgccccca acgggagcga gcccacgtcg cccatgaaga cggaggccgg
   173701 ataggtgttg aaccacaaaa agcccagact ggatcccagg atggctccgg tgaagatggc
   173761 gatttctccc gcgccttcga tgtaggggat aagcaaatat tcggccatca cacggttccc
   173821 ggtcacatag gccacgattg tatacgccag cgaggcgatc atgaccggac cgatcgccag
   173881 cccatcgagc ccgtccgtca aattgacggc gttggagctg ccgacaatga caagaatgac
   173941 gaacacgatg tagaaccagc cgaggtccgg cgtgaaaaat ttaaagaacg gcacgctgag
   174001 tttcgtcgta tagctgggta acgtgtagag aaagacgccg atggtcagcg cgaccagaat
   174061 ctggcaggtg aatttttgtg ccgccgagag gcctttcgat tggcgcttga tgaacttgag
   174121 atagtcatcc gcgaacccga tcgcgccgaa gcccaccgtc gccaccacga ccagccacac
   174181 gtatcggttg gtcatgtcgg cccacagcaa cgtggacagc accaccgcga agataatgag
   174241 t
//
LOCUS       DAORRQ010000002       158809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_2_length_158809_cov_222.777139, whole genome shotgun sequence.
ACCESSION   DAORRQ010000002 DAORRQ010000000
VERSION     DAORRQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 158809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 158809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..158809
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2_length_158809_cov_222.777139"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PK224_00770"
     CDS             <1..351
                     /locus_tag="PK224_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14303.1"
                     /translation="VTVLAGSLPALPWAVGLLVVTIAVMGFGNGVIFQIVSEWFPKDI
                     GLASGLVGAAGGIGGFLVPIGFGLLRESTGTFLFGFVGLAVVSAAAAMTVAPALRWRP
                     QAVAQAPSPGGFSR"
     gene            417..1625
                     /locus_tag="PK224_00775"
     CDS             417..1625
                     /locus_tag="PK224_00775"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRB14304.1"
                     /translation="MATPFNSIEDAIKDIKKGKFIILVDDEDRENEGDLVMAAGKVTP
                     HAVNFMAKHARGLICLALTPERVEELQLAPQAAENTATFGTAFTVSIDARKGITTGIS
                     AADRATTIHVAIDSRSTPGDLARPGHIFPLKSQVGGVLRRAGQTEGSVDLARLAGLYP
                     AGVICEIMNEDGTMARVPELTKFARRHKLKMVTIKALIEYRMHRETFVRRAASALLPT
                     TFGDFEAIAFENDIDGATHIALVKGQVDAETPMLVRVHSGCLTADVLGSLRCDCREQL
                     HRAMEIIQKDGRGVLLYLNQEGRGIGLLNKIRAYGLQDSGSDTVEANLQLGFKADLRD
                     YGVGAQILVQLGLHRIRLITNNPRKIVGIEGYGLKVVERVPIEIAPRADNEKYLRTKK
                     AKLGHLLKKV"
     gene            1787..2329
                     /gene="ribH"
                     /locus_tag="PK224_00780"
     CDS             1787..2329
                     /gene="ribH"
                     /locus_tag="PK224_00780"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRB14305.1"
                     /translation="MKLRKGSQDATGLTFGIVASKFNKFVTSRLLTECVKALTKAGVT
                     DEAIEVVRVPGAFEIPLVARQLARSGRFDAVICLGAVIRGDTPHFEYISTEVSRGILQ
                     ASMDSNIPVIFGVLTTDTVAQAIERADPEKLNRGADAAKTAIEMVNVMRLLKKEGSGE
                     APQPATPSRRAGRSAARPKR"
     gene            2343..2816
                     /gene="nusB"
                     /locus_tag="PK224_00785"
     CDS             2343..2816
                     /gene="nusB"
                     /locus_tag="PK224_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRB14306.1"
                     /translation="MASRHQARERAVQILFQYDVHGQAGPWLDDFWIQYPLAEELRVF
                     AERLVAGVRANKKELDALIGTYATNWKVSRMPIIDRNILRMGCFELLQVPEVPAKVTL
                     NEAIELAKSFGDEEASKFVNGILDKVLGSDARLERKRAFPELPVVGSDSSVKRET"
     gene            2893..4227
                     /gene="purB"
                     /locus_tag="PK224_00790"
     CDS             2893..4227
                     /gene="purB"
                     /locus_tag="PK224_00790"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRB14307.1"
                     /translation="MIDRYTRPRMSAIWDLQHKYEIWLEVELQACAAFERGGQIPRGT
                     SARIRKKAKIDVARIAEIEKVTKHDVIAFLESLMDSVGPEHRFLHMGLTSSDIVDTSL
                     AVQMTEALDLILEDLNELIAVLKKRALEHRHTVMVGRSHGIHGEPVSFGFKLAIWYEE
                     ACRHRTRLEAARKDIAVGKLSGAMGTFAHQGPEIEEYVCAKMGLASDPVSNQIVQRDR
                     HAAYASALALLAATIEKIATEIRHLQRTEVLEAEEYFSAGQKGSSAMPHKRNPIASEN
                     LCGLARLVRGNSLAALENVALWHERDISHSSVERVIMPDSTILVDYMLARVTDLVKNL
                     IVYPERMQRNLDLTGGLVYSQRLLLALIDKGAQRKESYEAVQRNAMMAWKGGVGFQEL
                     VARDPFITTHLKPHEITACFDPAYYLRHLDQVFRRVFGASGASSAKRRRKRA"
     gene            4224..5018
                     /locus_tag="PK224_00795"
     CDS             4224..5018
                     /locus_tag="PK224_00795"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRB14308.1"
                     /translation="MSLRSTARVGLLAGLLVLAAVWPVSAADREKLLTEPGVYGTFAT
                     FQMDHDWWDLPGESRVISVAEVKGLVEQWSGKILVESYLLRGLSDHADLMFRVHARTL
                     SDTQQFLSAFMGTRLGRHLTSGGLLHGVSKKPTYVAGFPESMKTELQVNGESGSRPYA
                     IVIPIKKDAEWWGLDQEARTALMQEHTQAALPYLKTVKRKLYHSTGLDDVDFITYFET
                     EKLEDFHNLVRALQQVKEFRHNRRFGHPTLLGTMSPLDEILEKFAQ"
     gene            5034..5747
                     /locus_tag="PK224_00800"
     CDS             5034..5747
                     /locus_tag="PK224_00800"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRB14309.1"
                     /translation="MTVEPAGTMIYEGKAKKIFTTDRPDQVLQYFKDDATAFNAQKRG
                     TIVDKGVVNNKVSERLFRLLEQHGISTHFIERVSDREMLTKRVTIVPVEVVVRNVVAG
                     SLAKRLGLKEGAAIDPAIIEFYYKDDALGDPLVNDDHLRLLNVATPAVLREMRELGHK
                     INAVLLPFFKERRMMLVDFKLEFGVFHNRLILADEISPDTCRFWDQTTKESMDKDRFR
                     KDLGKIEEAYQEVLKRVCE"
     gene            5755..5982
                     /locus_tag="PK224_00805"
     CDS             5755..5982
                     /locus_tag="PK224_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14310.1"
                     /translation="MNRFLQLFLNYGLVAAILVWAATVAMMAYHLKESPWRWAFVLMS
                     LAGLGTIGVIFWIRKYVQRVCKAQQEVGKAQ"
     gene            5979..6218
                     /gene="purS"
                     /locus_tag="PK224_00810"
     CDS             5979..6218
                     /gene="purS"
                     /locus_tag="PK224_00810"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRB14311.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAGNVRVGKYMEV
                     DVNEADRAKADAQVKQMCEKLLANTVIEDYRYELG"
     gene            6320..7042
                     /gene="purQ"
                     /locus_tag="PK224_00815"
     CDS             6320..7042
                     /gene="purQ"
                     /locus_tag="PK224_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRB14312.1"
                     /translation="MKIGVVVFPGSNCDHDCQYIFKDVLGQYVEMIWHKETLLAGLDA
                     VVLPGGFSYGDYLRTGAIARFSPVMGAVKAFANKGGLVIGICNGFQILLEAGLLPGVM
                     LRNTSLNFICKDVYVKVENAATRFTNQCESGQVLKIPIAHADGNYYTDPVTLGGIKAN
                     AQVIFRYCTADGKVTPDANPNGSLDNIAGIMNADGNVLGMMPHPERSAEAILGNEDGR
                     LIFQSMLSSFGKPELQSKLIGV"
     gene            7111..9357
                     /gene="purL"
                     /locus_tag="PK224_00820"
     CDS             7111..9357
                     /gene="purL"
                     /locus_tag="PK224_00820"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRB14313.1"
                     /translation="MNALVITKAILEQHKLSDDEYKKILEILGREPNWTELGMFSAMW
                     SEHCSYKSSRIHLKKLPTTGPRVVQGPGENAGAVDIGDGLCAVFKMESHNHPSFIEPY
                     QGAATGVGGILRDIFTMGARPIALLNSLRFGGLDNANTRHLLKGVVSGIAGYGNCMGV
                     PTVGGEIVFNDIYALNPLVNVFCLGIAKKDKIFLGKAAGVGNPVIYFGSKTGRDGIHG
                     ATMASDSFDEQAAQKRPTVQVGDPFTEKLLLEACLELMAGDCLVGIQDMGAAGLTSSA
                     CEMASREGTGVELELADVPRREPGMTPYEIMLSESQERMLMVVKAGREDEVIKICRKW
                     DLDVAVIGRVTDTGKVVLKENGQMVAEIPAKALADDGPRYDRPSAPPAYQEMLQALNH
                     DALPDVKDVNAALLALLDSPTIASKRWVYEQYDHMVRTNTMVRPGSDAAVVRVKGTNK
                     ALALSVDCNGRYCLLNPYEGAKIAIAECARNLACSGAQPIGVTDCLNFGNPQRPEVMW
                     QFVLAIEGLKDACEALNVPIVSGNVSLYNETNDLSIYPTPMIGMVGLIEEADRAVTQW
                     FKENGDTIILLGQTREDLGGTEYLKVLHHREQGSPPLLSLETEKAVQACTIRLIRDGL
                     VQSAHDCSDGGLAVALTECCISGSAQQLGAVVQLSLDGLRRDALLFGESQSRILLSVK
                     AELAERVLNAAWDAGVHAARIGTVGGARLVIQVDGDQRAAGCKIDLELTALYDHWGLA
                     IPRAVGQD"
     gene            9370..10800
                     /gene="purF"
                     /locus_tag="PK224_00825"
     CDS             9370..10800
                     /gene="purF"
                     /locus_tag="PK224_00825"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRB14314.1"
                     /translation="MANELPLISPDKFHDECAVFGIYGHKEAANLAYLGLYALQHRGQ
                     EASGIVSNDGEQFYVEKGQGLVADIFSQQALARLPGTMAIGHNRYSTAGGAGLKNVQP
                     LSVNFAFGNLAVAHNGNLINATMLRSELEAYGAIFQSTSDTEVIIHLIAHSRADTLLD
                     RVIDSLTQVRGAFSVVLMTDQGIVAARDPHGFRPLCLGRFRDAWIVASESCAFDLLDA
                     EYVREIEPGELVVLDHQGVTSYKPFAQTKPAMCVFEYVYFARPDSRIFGGKAVYSIRK
                     AFGRQLAQESRVDADIVIPVPDSGVPAALGYSEGSGFPFETGLIRNHYVGRTFIEPEQ
                     SIRHFGVKVKLNAVPEVLEGKRVVVVDDSLVRGTTSRKIVKMLRHAGAKEVHMRISSP
                     PIVSPCFYGIDTPTKKELIASSHTTEEIRKYITADSLAYLSLDGMVKAAPGTPGQYCD
                     ACFTEQYPISFTRAEELQLGLFEAPR"
     gene            10868..11308
                     /locus_tag="PK224_00830"
     CDS             10868..11308
                     /locus_tag="PK224_00830"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HRB14315.1"
                     /translation="MSLVRKYRVIVATGVVVVGVRIASRLTSLRRLLGWLVCEISADP
                     DEHAMEDVAYYVDRWLALFPYNPKGNCFPRALALFFFARRAGLPVQFKCGVMKLNQQL
                     EGHAWLVLEGHEFLEPSSYWQSFTVTVTFPQSLVSSSTQSSRIS"
     gene            11683..12000
                     /locus_tag="PK224_00835"
     CDS             11683..12000
                     /locus_tag="PK224_00835"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRB14316.1"
                     /translation="MPATVHLWSEPQLELSEMFPRQDEQVHSTVLDGESVLLNLNSGR
                     YYTLNVVGSTIWNLCRGEQSLRQIHSAICARFDVSEQQAQDDMLALIVLLEQEGLLHT
                     ERR"
     gene            12242..13276
                     /locus_tag="PK224_00840"
     CDS             12242..13276
                     /locus_tag="PK224_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14317.1"
                     /translation="MASTMKELLLSVHGIEMKFTTNSSELAHSVWALLRHFHRDTVAA
                     QPMRMEFDAIERREDVPAQISSSAKLLYSGSGLAIDPDRKTTWLCDIYSDNGLLIADF
                     HGEGLVVIDGDSQSARGYLVRPEAMAPDIRASFVHFAMTELLKRRGLYTFHATSLEHK
                     GQGVLIPGFSGRGKTTSFLSLLRAGYRYLSDDHPFFRISGTRVEILPYPLKINVTDHT
                     VSFFPELREAPPSVLHAGVPKSYFHAEDVYPGPLGQPCEPSVILFPEVVNSSHSCLEP
                     LSKKVALEMILPHSLLVYDTEVARREFQALVGLVEQADCYRLHFGRDVMELPWLVTPL
                     LEKRQARREN"
     gene            13377..14573
                     /locus_tag="PK224_00845"
     CDS             13377..14573
                     /locus_tag="PK224_00845"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRB14318.1"
                     /translation="MRTEGRLLALCARTTVSEFVRVEVADLACDGIDWELVWQLSRAH
                     GVAPLVYRNLVTICPAAVPSSIHEAFRRHNQANALLNSLLAKELVTLLDALAAKGVTA
                     IPFKGVTLAQTAYGDLGLRECADIDLIVEQGAIPQARKVLWSQGYQLTSQDMGGGEES
                     GEPYHFFQRRNGIVAVDLQWMMARRHFGFRLDRSAFWGRLKPVHLPTKSVMGLCPEDL
                     LILLCVHGSKHAWEQLKWVCDVAELVRRRPALDWSRVLFQAGEWRCRRLVLLGLAMAH
                     SLFDIAVPLTILQEIETDADIPVLVRKMPKQLLKNPRHGIDEDCAEALYMTLKDSWLE
                     RWKLGVALCRAEAEVLTRSLPWFRVQRRLRMLATCLKPFHRIIAKWILSVRMREAVVR
                     WLKSSG"
     gene            14764..15309
                     /locus_tag="PK224_00850"
     CDS             14764..15309
                     /locus_tag="PK224_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB14319.1"
                     /translation="MKRLLVAVAIVLVHLWAAHLVVAAGFFKVGEPAPTFSLTSVTGD
                     AVSLEQLKGKVVVLGLFHICDPCMTQGTNLQKVHEAMTGKNVAVVGVNSSGDSKRGVG
                     EFLSAFPVKVTYPYLLDPNKTTDKLYGGGKFIPNVYVIDQRGVIRWQRVGNMELAGAE
                     VIIQEVEKLLAASTPAGAGTM"
     gene            15329..15568
                     /locus_tag="PK224_00855"
     CDS             15329..15568
                     /locus_tag="PK224_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14320.1"
                     /translation="MDEWEEGKQKFLEVVQSIDQTVQVVIPTKPTNSMFLISLTKGPN
                     RKFITLSEDDIVDLPSEAGICAKVTKTLQDAVAAL"
     gene            15593..16189
                     /locus_tag="PK224_00860"
     CDS             15593..16189
                     /locus_tag="PK224_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14321.1"
                     /translation="MKQLLSGIWQWSWFSEDKQLDFNGLFLNVGEHKILIDPPPMTAE
                     AHTFVRRQGALDYIIVTNRDHVREAASYQADFRCQLKVPEADVAQMDVKPSKTFKDGE
                     LLPGGIWVIHLNDQKSPGESALYIQQGKGVLIVGDALIGKPAGGLSLLAPEKYGDITK
                     ARDGLRRLLKYNFDSILVGDGASILFGAKQAVEQALQT"
     gene            16196..17020
                     /locus_tag="PK224_00865"
     CDS             16196..17020
                     /locus_tag="PK224_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB14322.1"
                     /translation="MALRNGLSEELNRQGNEHFARGHYTDAYACYAKALECDRLTGDH
                     RALSATLGNLGNICAVSGRRESAQSYYQEVLELQKVLGDEKGIGTTLANLGNLRADAG
                     EWDRARAYYLEALDIMTRVHDELGKAVLFSDLGLVARETGQFEEALRYYEQSLVFMRR
                     LNNQGGVADAWRMMGRTFAIQKRYDDAIACCHTSQSIAERSRDELRTGGARYVLAQCY
                     EDLGQLQMAVQLLEQVVRMDRKYDLPKLAENVTRLERLRARLGAEDPTPQPRESRA"
     gene            17017..17454
                     /locus_tag="PK224_00870"
     CDS             17017..17454
                     /locus_tag="PK224_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14323.1"
                     /translation="MNAVALPSWEQARARLVSTVPQFYELEPGGALALDLGDDGWLLE
                     VRSDGQLLCQHGIAMDDVKSLMCDGTTEDLGTDEVAKQAKYFLGPAVSKKRPALLKAG
                     FAEKTDMNDEYVAITFHKTVDFQRFDEVLAAVRWCQNLFKIEP"
     gene            17485..17898
                     /locus_tag="PK224_00875"
     CDS             17485..17898
                     /locus_tag="PK224_00875"
                     /inference="COORDINATES: protein motif:HMM:NF028174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HRB14324.1"
                     /translation="MRPPITSIETFHRLLAACRLPRVIVTAPELSLLTAMGSGSWSIQ
                     KLAQALDVSERGVDILCRNLAMCGLLRKRGKAYRNTPFSATVLNAKHPKYRGAYVELL
                     QSHWVDWSRLTRVVRTGSPLERDQPDLLAYRRRFT"
     gene            complement(18282..18836)
                     /locus_tag="PK224_00880"
     CDS             complement(18282..18836)
                     /locus_tag="PK224_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14325.1"
                     /translation="MIASSGELGLAGTGGSLVTSPQTNRLALEPGARELAMGNRGSYS
                     ASMKAFRPVITLLVALVVLEGLLFEAGSANSLATIRDGILLGFLFGVPTLLVAGILLL
                     RQHWVVMAAVIYSTIALALDLSTIVQEASQASPRPPILALTLGSSLLNFLLMIFGGRC
                     ALSFRPDESPPTPPHPNLQSPSSS"
     gene            19149..19886
                     /locus_tag="PK224_00885"
     CDS             19149..19886
                     /locus_tag="PK224_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14326.1"
                     /translation="MAQVPADHSEVLAAGFGFQAAGSSMITVRTYDAPTGAILSEDSF
                     DVNVKEEGVAEHDGNKGRIFAGGIGTDSKGKSVFMLRVYDAETGRFLWEGQLNLLKLG
                     EGGMTRASATIIPTRTSALQVSVSSTIPLQTLFSVRAVNPFTGGLVWQDQFAPGVRKR
                     ARVEGVLFGGLFVRPVGDPIGHIFDLVVRTYDRTSGTLLWEDSFEQLDRIEESPTEPE
                     LRAHPQAIPSWNLSAAPRHHAYQTKLR"
     gene            complement(19889..21331)
                     /locus_tag="PK224_00890"
     CDS             complement(19889..21331)
                     /locus_tag="PK224_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB14327.1"
                     /translation="MYRRNIKHILGPLAVLIGIFIFYQTWLKPRYFHDTPAPSPQVIV
                     EETTPASNAPVAPSQERTAPEIKRSRTIDPVSIPVPRHPELLGAIHEELEKHNIALAE
                     TKLEELPSSILTESATKRHVAILWNNLGIIQERSGGTEVSVKAFKKAVALDPQNPIAH
                     LNLAHAYWGLRDPALTEDFLRKVVALSPDEPFPHVALADLLQEKDHLPEAGKHLAQAK
                     ERIKKDPGLQSYLKAVTTKVHRAEKVEDKFSTHSSVHFTVKYDGGDDPDTWTTVLDIL
                     EEAYREIGQKFNFFPSKPIIVVLHTKNQFQGATGSPGWADGLFDPVLGRIQIPTQGAA
                     TDRAWLTRVLRHEFVHALIHEELGATGGMIPTWLNEGLAMQLAGDPWQEVTSVTPGEH
                     SLVPLTALEGSWESLPAEKFSLAYREANAATHYLIERFGMHKVHEVLVHLKARQTIAA
                     AMQDRLLLSYDHFQQQWADSLGSTLAQPKS"
     gene            21494..24034
                     /gene="recG"
                     /locus_tag="PK224_00895"
     CDS             21494..24034
                     /gene="recG"
                     /locus_tag="PK224_00895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRB14328.1"
                     /translation="MPRPESADSHDPFQAFLHRVTRPIEFACRDAYAHLSAVRNLDRF
                     VSQQVIGTLGERVYPRALETELIALRNLFVDFHTRLTPLEQQDRLTKALALLSRLQGD
                     ARAVSQPVGSPKEKQVQPLPVRSSPARPLWELSIQYAKGVGPKRTLLLERLGAHTVEQ
                     ALWTLPWRYEDRSVITPVAELVPGAIRSVCGVITRAEATRARVRRLSILDVAVQDATG
                     TVHAVFFNQPYLEDVLKEGLRVMMSGRVAAGRGGWTDVRLEATQFEVLSGGEDELLHV
                     GRIVPIYHETKGWTSRQMRVLMQGLLAEYGADIEEVLPLSVRARHRLPPIGEAIQHVH
                     FPLPKTDLAALDQGVTPAHRRLAFEELFLLQAAMVLRQREMKEELKSFRFNPHVAQLK
                     QLAKILPFTLTSAQERVFREIQADMVTSRPMNRLVQGDVGSGKTVVALHALVMACGSG
                     CQTALMVPTEILAEQHYLNLAPLLRAVGLKAVLLTSGGKAKDRNETLKQLESGVAQIA
                     IGTHALIQKKVRFANLGLVIVDEQHKFGVLQRKTLLEKGYRPDVLVMTATPIPRTLAM
                     TVYGDLDVSVIDMLPPGRKPVRTLLYTDGQRHKTWQLVGDELKAGRQAYIVYPLVEES
                     EKVDLKAAIQGAEHLQREVFPQARVGLLHGRLPTAEKERTMAAFKAGTIQVLVATTVI
                     EVGVDVPNATVMVIEHAERFGLAQLHQLRGRVGRGAHQSLCILMATYLAREARTRVSR
                     DGRPEQNASNAQQRLAALVNSNDGFVIAEEDLRIRGPGEFLGLRQWGVPEFRAANLVR
                     DAQLLEQARQEAVTLLEEDPGLMLPQHQAFKAAMFRRWRGRLALGDVS"
     gene            24072..24398
                     /locus_tag="PK224_00900"
     CDS             24072..24398
                     /locus_tag="PK224_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14329.1"
                     /translation="MGMLQRLSHDLRAGWVTLRHGTAKAATRALEEGELLRYRLELRK
                     VDQQLDDLHGDIGERTIALHDRGESADRILSDSEITRLMQQVRTLQDEHTKLLLEMND
                     ITVEGQ"
     gene            24409..25359
                     /locus_tag="PK224_00905"
     CDS             24409..25359
                     /locus_tag="PK224_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRB14330.1"
                     /translation="MTNQTTASPRIFAGIDIGTLTCRLLIAELTSAGGLRELRSDRRI
                     LRLGQGVDRERVLRPDAMARVVATLKEWRQVIDGYHVDAATAVATSAVRDAGNRDEFL
                     GLVQREAGFQVEIISGEEEARRTMLGIRSGLPSGVTDLLALDIGGGSTEFILDRPGQA
                     TLARSIDIGVVRLSERILHSDPPTPDEVQEARDWVRRETTAAIAAMPRSPGVMFVGTA
                     GTITALAAMVQQLPAYEPARIHNYTLTLNMVKQLELQLLSCSKAQREGLPGLERGRED
                     VIAAGAVILGTVMDTLGMTKVLVSDLGLREGVLIHLAGGR"
     gene            complement(25527..27020)
                     /locus_tag="PK224_00910"
     CDS             complement(25527..27020)
                     /locus_tag="PK224_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB14331.1"
                     /translation="MKAAAAIQEPLTLSGDTLNLLAWRIPDLVAYQHAFDEWWLAPLD
                     DDFPLVRLNAVGIEMLTSMNGHITVGALVEKYGNKICGPDGQPGTWHLARWSTPNYSL
                     CYFGTEPPGGHRHKAKWDLLLQQIREGWSGQQEFEGEEHLEDFHVHELKESDLEDGHF
                     DLIETTVSHLFREPCEALGGTTYGRLLMRQLRRLGWFKPKPKVIVEIGGGLGYVAREL
                     GQELLPFEKQGIQYISLDVTRPFLQLQTKRAKAGGWTGTGTHANGECLPFRDNSVDLI
                     IDNENMADMTPVKLSRKELTEGTGETAQHQEALDWIRRLKLPLEKELPEEVIFNLGPM
                     RFVAEVWRVLKPGGRAFLTEFGVEEGWPAAVKLPHHTEYEVQYNHLRQAVRWLGFQER
                     YLSLPQFLQIKPDTKVLCTGAAYTIQRFCQGLGQNFSVRAYTESELTKTLGDILPKLQ
                     GCHYHDIADPAWFGLIDFKVLLLEKPGGIPQPTFTEQKSGLRWYSQR"
     gene            complement(27102..27476)
                     /locus_tag="PK224_00915"
     CDS             complement(27102..27476)
                     /locus_tag="PK224_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRB14332.1"
                     /translation="MSWRLFFFVLATSTLIAWPTSATPPATQVLMDSGSPYYVPAAVT
                     VTAGGAIRWENPTPTHHTVTHDACLEETGRCAFDSGAVEPGGAYTIPSLPPGRYPYQC
                     RIHPIMRGMIIVTESPLLPSQT"
     gene            27785..28378
                     /locus_tag="PK224_00920"
     CDS             27785..28378
                     /locus_tag="PK224_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14333.1"
                     /translation="MAKKIVKKKSVAAKASPPKKVAAKKATKLKKAVAAKKPVKAKKA
                     VAVKGKPAAKKVVKPARKVAQKPAAAAKRPSKAKPVSNAVKPAPKAVKPAAKPKPKPA
                     PPKVNPPVEQSAVIPESKPAKPPVPEVSKAAKVEPEPQPLELDDEEEMAEDLEDVSEE
                     IEEEIEEELGLDGEDDDVDYLEKSEDLLDDADEYRNN"
     gene            28580..29032
                     /locus_tag="PK224_00925"
     CDS             28580..29032
                     /locus_tag="PK224_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14334.1"
                     /translation="MSHFLFIAGALRERTSQESAELQFGAGIWGLCTALIRDNLQKFL
                     APDSHGLVYVLKEGIRAEFAITSPVQPFQALDELLKDEVRTEARYGFVRITPLRRWQV
                     HAPDCQALLQRVLGIEEQAELTRRLSLGMHRLTDAEFRAIVEGLGVEA"
     gene            complement(29061..29741)
                     /locus_tag="PK224_00930"
     CDS             complement(29061..29741)
                     /locus_tag="PK224_00930"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14335.1"
                     /translation="MSAITPVVHTSGGRRFLCTVFACTVVLLATIPRAGAEAVVEVPM
                     LAAIQSNNLGVFEVLLLRWDHRPSPSPMELQWLGGNIRPGQTNLSSMVLAFQYALAHT
                     PQIDHAGTVSAMGIAYAATGTDGPSAGAVMTVGFAALLKGDHIRRGIALTGTIAKDGT
                     IGPVGGIPDKIRAAAREGYRTILIPQGQRDDPRWNLNRLAWELNVEVTEVATVEEAYR
                     LMTGGTFE"
     gene            29947..30933
                     /locus_tag="PK224_00935"
     CDS             29947..30933
                     /locus_tag="PK224_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRB14336.1"
                     /translation="MKNAAAVFTLHLPNTCTEAEREARAVSAQELLRALAAGKGLDLS
                     GVVIQGDLVLDELPAQKVSMVGDLAPEDRRVLEGLNDEEVHVIRGPFVIKQSRVKGRI
                     VNRLKSGFLLITGPVVLAHTDFAGFVDLSRTVFLGLVDGSNATFHQESYFVQDRFTQG
                     AMFSDTHFGPHARFHRSVFAGPAIFRGATFQGLTEFLEVVFEQDANFSRAAFHLGTGF
                     SGAHCRAKCDFSSSQFDREAFFLFAIFDRPATFASARFGSQADFSDAAFKQADDLAQA
                     TFARTPQLTRTARVSTTVPGPTASTSAPFSQAVTIILFVMALGLLVYIIRAK"
     gene            31032..31811
                     /locus_tag="PK224_00940"
     CDS             31032..31811
                     /locus_tag="PK224_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRB14337.1"
                     /translation="MDQPELPYQVKIENFEGPLDLLLHLIKKNEINIYDIPIALIAQQ
                     YLDYLSVMKELNLAVAGEFLVMAATLLHIKSRMLLPVDEVAVDEEDGPDPREELVRRL
                     LEYKQFKEAASQLDYKERLWRDVFSREPGPPVEVKKDESLLDEISLFDLVDALQGVLS
                     RHPGKRLVEIIPDNLTVRTRMSAIIEALELHESMAFTALFEESSHRLVVIVTFLALLE
                     LIRIRVVRVFQSETFGAILVSRAFSAVAEGELVEESEWKKL"
     gene            31796..32713
                     /gene="scpB"
                     /locus_tag="PK224_00945"
     CDS             31796..32713
                     /gene="scpB"
                     /locus_tag="PK224_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRB14338.1"
                     /translation="MEETMSPGVEEAIEESVEVRDPVDEPTASLDAVEVEPEVGQDVI
                     TEMSAPAADPALSDMRALKGILEALLFVTAEPIPVTRFLALLGAVTKQDVDQALASLA
                     QDYEQEGRGLQLAEVAGGYRIVTKAEFAPWLKRLEKVKSPSKLSRSALESLAIIAYKQ
                     PIVRAEVEQIRGVETSGVIRTLLERKLVRIVGRKEEPGRPIMYGTTKFFLEHFGLRDL
                     SQLPPLREFKELGESEQAMLPIDEVVAVDGEPVTQSTEAVVEGLSEGMDEPVALLEPV
                     DEAGQTSESLVLENEAGGDLVVADIAEES"
     gene            complement(32882..33448)
                     /locus_tag="PK224_00950"
     CDS             complement(32882..33448)
                     /locus_tag="PK224_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14339.1"
                     /translation="MWPTRLVLATLAASLLLTPSVHAVESSPVEMWECPGPEGTILYT
                     NKERPDCQAKVLKALSVVPSLPDLPLAPPNGSTYQPTPKAKDFSYDTPIGALRNLSQI
                     PESGRDWYAGNTSGGSVQGEVCSMYMEWIHLNQNSRGGHFFGTDPSYGSNPTARNWYT
                     PSYSFYDNARYVTLSQIFGAGFVPIGCH"
     gene            33775..36744
                     /locus_tag="PK224_00955"
     CDS             33775..36744
                     /locus_tag="PK224_00955"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRB14340.1"
                     /translation="MIKAWLLDEMFRFDRRYLTAEGSQSEWGAGDSVAEEEDLPPAPT
                     GVAAQAGNGRVMITWDAVPEAMYYNLYFMTTKGVQIKFSELTRPIASAEDFKTVIGVT
                     KEKGTCIEGAQSPFVHDDLANGTCYHYVVTVVTQKGESPESQEVMAIPAPYLIAKIIG
                     QEGVEDGEFSSPTGIAVDKDGNIYVADTDNHSIQKFDKDGKFLGRWGGEAGSAEGGFY
                     YPRGLTTNSDGQVYVADTGNNRVQRLDTDGNPMKAWGKFGFAWRGADMNKFDAPWGVT
                     TDQDGNIYVTDTNNARIQKFKGDGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNTYVAD
                     EGNNRIQKFDTRGSFLTKWGREGSGPGQFKAPWGVTCDALGNVYVVDQGNHRIQKFDG
                     NGTFLCAWGNRGKTEGQLNFPSGVAVDKEGAVYVVDSGNHRVIKYVPTDEELNRGKAE
                     RERAQAVSEYPIPRNLAIKPGDTETFLSWMDVPGAVSYNLYFHTSANLTQEGGTKIEG
                     VTSPYNHSGLTNDQAYYYALTAVYEDGTESGLSDEVSATPVLIDITAPQTPYAVINHG
                     AFMTNSPEIVVTISATDLDTGVAAYYISENPMTPMAGTPGWVEVPPAIKFGATIPFIL
                     SPGDGQKTVIVWFKDLGNNISTPASATILVNTSGYLCVSKWGKPGRGASLLHGGEFMA
                     PMYGLAIDQQGSIFVVDNGNNRIQKFDRTGNFIILWGNFGAANANFHNPTGIACDAKG
                     DVYVVDTNNHRVQKFDGKLGGYLMKFGSRGNGEGQFNAPWGIAIDRVRGYIYVVDSAN
                     FRVQKFDMSGEFIMSWGSFGNGDGQFYFPRGIAVDQADGTVYVVDMGNHRVQKFDTST
                     NVLPQLLTKWGGSSEAGHASSPLAQEAGQLRSPWGIAVDGQGDVYVTDTGNHRVEKFD
                     REGNFIAQWGGFGGGDGQFNFPYGIVVDSRGSVFAVDSGNTRVQQFMPADEGSERLQE
                     EADAAAELGQVDKTTRV"
     gene            36783..38249
                     /gene="guaB"
                     /locus_tag="PK224_00960"
     CDS             36783..38249
                     /gene="guaB"
                     /locus_tag="PK224_00960"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRB14341.1"
                     /translation="MLEKEIRLGLTYDDVVLVPAKSQVLPSEVDTRTRLSRNIQLNIP
                     IVSAAMDTVTEARLAIAMAQEGGIGIVHRVLSPTDQAAEIDKVKKSESGMILDPITIS
                     PDQTIRDAHDLMARYRISGIPVTKSGKLVGILTNRDLRFETRMELKVSQVMKRDKLIT
                     APEGTSLEKAREILHEHRIEKLPVVNKQFDLKGLITIKDIEKRIKYPNACKDAHGRLR
                     VGAALGVGPDTADRVALLVKAGVDVVVVDTAHGHSQAVLDTVKMVRKAHPKLDIIAGN
                     IATAQAAKDLVKAGVDAVKVGVGPGSICTTRMVSGAGMPQLTAIADCARALAGSGVPV
                     IADGGIKYSGDITKALAAGASVVMLGSLLAGTEEAPGETVLFQARTYKVYRGMGSIGA
                     MERGGGDRYGQGGRPTPKLVPEGIEGRVPYKGLLAPHIYQMVGGVKSGMGYCGCKTIP
                     DLQQKATFIRQTVAGLREGHVHDVIITKEAPNYRTDWE"
     gene            38341..39897
                     /gene="guaA"
                     /locus_tag="PK224_00965"
     CDS             38341..39897
                     /gene="guaA"
                     /locus_tag="PK224_00965"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRB14342.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVPLATV
                     LAYRPKGIVLSGGPSSVYDKKAPSVSKQLFEQGIPILGICYGMQLVTHLQGGEVEKAP
                     HREFGRAELTLDDRSDLFKGIGTRGSTVVWMSHGDRIERMPPGFRSIAHTDNSPIAAM
                     KRHDARQRIYCLQFHPEVAHTTEGATILKNFVYDICGCKPTWTMRSYVDTAVEQIRQQ
                     VGTERVICALSGGVDSSVAAALTHRAVGDQLTCIFVDNGVLRTGERDQVEKTFASQMH
                     LNMRILDRTSQFLTGLKSVTDPERKRKIIGRLFIKNFEVESKKLKGIKYLVQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPTRMKLKLVEPLRELFKDEVRALGQELGLPDEIIW
                     RQPFPGPGLAIRVLGAVTKERLAILRGAETIVDQEIRAAGLYREIWQAFAVLLPIRTV
                     GVMGDQRTYEYVIAIRAVTSLDGMTADWAKIPSDVLGKMSSRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            39993..41255
                     /locus_tag="PK224_00970"
     CDS             39993..41255
                     /locus_tag="PK224_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB14343.1"
                     /translation="MEVRLQKLIAGTGLASRRKAEELITSGRVTINGSVVKELGTKVD
                     PARDHVKVDGKHLRAAQPYVYLILNKPKNVMSTLDDPEGRPTVKDLLHGVTVRVFPVG
                     RLDFDSEGLMLLTNHGDLAQALLHPRYHVPKTYLIKVKGVLDDAKIEALERGVKLEDG
                     FTAPAKVNKVSKAESNSWLEVTIHEGRKHQVKRMVEAVGHSVIKLVRVRMGPLLLGDL
                     APREYRFLTDREANRLRDLVEDRVARAERPELDASGKPTRWMPPTEPAPPRPPKPERS
                     GRGPKAQRSERGPRSQRSLASAKPSRSGRGTTRRPMSPGAKFRRPAKGVGFQRTQAGP
                     IPQGADRGPGSRPTGPDQKPQRTQPGAGFQRPRSGAKPQGPGRGMTVRRSGPGQNFQQ
                     KRRGGSSASRPGQRGPAGKSRPSTRRRA"
     gene            41252..43015
                     /gene="aspS"
                     /locus_tag="PK224_00975"
     CDS             41252..43015
                     /gene="aspS"
                     /locus_tag="PK224_00975"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRB14344.1"
                     /translation="MKIRTHRCGELTKAQVGQQVVLNGWVQRRRDHGMVLFIDLRDRT
                     GLTQVVFNAERNAAVHQAAHTLRSECVVSVTGQVMARPDESKNPNLPTGEIEVFVDAV
                     EILNEAKTPPFMIEDDIEITESLRLKYRYLDLRRPRMQRLLGLRHGIMQAVRAFLNAE
                     QFLEVETPVLTKSTPEGARDYLVPSRVNPGMFYALPQSPQLFKQVLMVSGVDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLEMSFVDREQVMTLMEHMIVSVFKDAGGVQLPTPFPRM
                     TYADAMGRYGSDKPDLRFDMPLHDVTAFAAKSEFKVFKEAATKGGLVKALIVSGGASI
                     ARSRIDALGETAKSFGAKGLAWLKVTADGQLESVIAKFLDAKAFAEALPEAKPGDLVL
                     FGADKPAIVHDVLGRIRLLLGEELNLIDKTAWKPLWVTEFPLLDYSPEEKRYIFMHNP
                     FAAPMDEDLPFLDTEPLKARAKAYDMVLNGSEIGGGSIRNHRSDIQLRILDLLGIGKE
                     QAQAKFGFLLEALEYGAPPHGGIAFGLDRLIMLLGGADSIRDVIAFPKTQRAQCPLTE
                     APSGVSAEQLKELRIKLDLVE"
     gene            43034..44170
                     /gene="aroC"
                     /locus_tag="PK224_00980"
     CDS             43034..44170
                     /gene="aroC"
                     /locus_tag="PK224_00980"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRB14345.1"
                     /translation="MAGNTLGRVFTVTSFGESHGPAIGCVVDGCPPGLALSAEDIQRD
                     LDRRKPGTSRHVTQRQESDTVEILSGVFEGKTTGTPIALLIRNEDQRSRDYGNLLDTF
                     RPGHADYTYWQKYGIRDHRGGGRSSARETAVRVAAAAIAKKWLQEKHGVVIRGYLSQL
                     GPIEAPFQSWETVDTNPFFSANAEVVGKLESFMDELRKAGDSVGAKITAMAEHVPVGW
                     GAPVYAKLDADLAGAMMSINAVKAVEIGAGFASVAQRGSEHGDELTPEGFVTNHAGGI
                     LGGISTGQDVVVTIAIKPTSSIRIPRRSIDKQGNPVTVETNGRHDPCVGIRATPIAEA
                     MMALVLMDHALLHRAQNADVQTATPKIPGSSTHGAVPASKKPTA"
     gene            44284..44754
                     /gene="rnhA"
                     /locus_tag="PK224_00985"
     CDS             44284..44754
                     /gene="rnhA"
                     /locus_tag="PK224_00985"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRB14346.1"
                     /translation="MIEIYTDGACSGNPGPGGWGALLRIDNAETELCGGNPATTNNRM
                     ELLAVIEALQSLTQPVEARVYTDSQYVQKGISEWIHSWKRRGWKTAGKEPVKNEDLWR
                     RLDTLASGHKLEWHWVRGHNGHPENERVDALARAGLEQSRRAGKTVGGTRQGLF"
     gene            44797..45594
                     /locus_tag="PK224_00990"
     CDS             44797..45594
                     /locus_tag="PK224_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB14347.1"
                     /translation="MAIPFPILDIQHATVYRGETRVFSDFSFRLNAGEHAAILGPNGA
                     GKSTLLKLLSGEVHPLALEETRTRLFGEERWSMWDVRKKLGIVSYDLQRDYLICAEGR
                     NVVLSGFYASNDTYDHQTFTRDQVSRADAVMRELGIESFATRVFGQLSTGEQRRFLLG
                     RALVHDPPVLVLDEPTSGLDLKSCFQYLDLVRTQIHKGKTVVLVTHHLHEIPPEIDRV
                     IFLKGGKILADGPKLNLLTDDRISTLFDRPIVLAQANGWYQALPGLS"
     gene            complement(45604..46638)
                     /locus_tag="PK224_00995"
     CDS             complement(45604..46638)
                     /locus_tag="PK224_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRB14348.1"
                     /translation="MSRSSLDYVKQIVRGSLGMMAAVSLATGLFTNEAAAIEATMLER
                     AKLATIGVLEDTQDQRTPDKPGKILVRGTGFHLRDGYIVTARHAAEKQDVTTGTVIQK
                     QIHVLTTDLHEIPADLVGDSAFMDVVVYRVAEAHRAKLRATVPFASGDVQPGQEVFTV
                     GYPMGWGPTMSFGHLGNTNTFLQTVDTRLIQADVAACSGNSGGGLFNDKGEVVGIMHA
                     IIQTERDDSTAHCSRMAFAIPAMLAERIVTAALEGKPMAFSKMGIHMVAVKDGTRWRM
                     AVKDVMEPAKSAGIEKHDIIIAVDDTDIQDAAHLKNYLIERTKPGQQVSVKVRRIDTN
                     LTFTVTLAGG"
     gene            46759..47349
                     /locus_tag="PK224_01000"
     CDS             46759..47349
                     /locus_tag="PK224_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HRB14349.1"
                     /translation="MASSCDGRYTELMLLGTRQYLILGLLAVAAGCATSVIPESLEPQ
                     VDKTVTFSQVLEAPDSYRGKVVVWAGEVLKAKAMKGGTQLEVLQLPLDDEQGPVTDRM
                     ESKGRFLALQKEFLDPATMTDGTRVTIVGEITGASVEKIDEADYRFPTLDVKHLHRWD
                     SRQVDDRRVIGPWWNVFGGVGFGGGSRSGGGISIGF"
     gene            complement(47458..47913)
                     /locus_tag="PK224_01005"
     CDS             complement(47458..47913)
                     /locus_tag="PK224_01005"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRB14350.1"
                     /translation="MTKWPSDFDEMAQRLGAPGDFAQAYVIAHEIGHHVQNLLGIAGK
                     VTRLQQRGSTAEHNALSVRMELQADCFAGVWSYHAQHNRNLIEPGDFEEGLRAAAAIG
                     DDRLQKMGQGHVQPESWTHGSSEQRMTWLRRGLQSGDPNNCDTFSGGRT"
     gene            complement(47888..48346)
                     /locus_tag="PK224_01010"
     CDS             complement(47888..48346)
                     /locus_tag="PK224_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRB14351.1"
                     /translation="MRWEGQRESRNIEDRRGMGAARSGVGLGLGGIVLVLAVSFLTGT
                     NPLTLLNLLNGVQEITAPSEPERPSPKGAPRDSLGKFASVVLADTEDTWRVLLPRLGS
                     TYEDPQMVLFTGAVRSACGTASSAVGPFYCPGDQKVYLDLSFFDEMAQRL"
     gene            48642..49259
                     /locus_tag="PK224_01015"
     CDS             48642..49259
                     /locus_tag="PK224_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14352.1"
                     /translation="MTTVTNPMHRQAPTRARFIVLALAGLLSLSGCQSPGTPLPPTLK
                     PSLAWPECPKIGGTYELQGEPLPGTMNFYRNRDNKLTLNAMLGVAVPADLAQEAVRVE
                     LVHEDTLELVNRFDGAITAHQLDLQPEDRVTCDRNQIRIHRTRSEGGVAEEPLRNISD
                     IAQELTLEGDGSLLVKTRIMNQSKSAFFSTPVPHEEYSARFRRLD"
     gene            49389..49976
                     /locus_tag="PK224_01020"
     CDS             49389..49976
                     /locus_tag="PK224_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB14353.1"
                     /translation="MTVHRTARTLVFNKPYDVLPCFTDPEGRQTLGDYIDVSGVYAAG
                     RLDRDSEGLLLLTSDGALAHRITDPEHHLPKVYLVQVERIPDAAAVERLQQGVVVGGR
                     RTKPARVRLLLEAPQIPERPVPIRFRKHVPTAWLELTLREGMNRQVRRMTAAVGHPTL
                     RLVRVAIGPIFLGALLPGEWRELTKAETAEIYGYS"
     gene            50120..50581
                     /locus_tag="PK224_01025"
     CDS             50120..50581
                     /locus_tag="PK224_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRB14354.1"
                     /translation="MNTRDRIIVGLCSVLLGLLVLVSPAHADDTLVTKQSHYTVAETI
                     DRIARAVTDKGMLLFARINHADEARKVGFEMPPTELLLFGNPRGGTALMLAAPTVAID
                     LPMKALAWQDQAGRVWLTYNAPTLLPVRHGLAAELAARLDPVGTLLERAVE"
     gene            50764..51306
                     /locus_tag="PK224_01030"
     CDS             50764..51306
                     /locus_tag="PK224_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14355.1"
                     /translation="MRSQTIAVGRVSLLLVLCGIGLLPTVSALGANPESVPLSDMRHI
                     KKFVAVEVQTQGTAEKLGINGAELTDVTRATLLNKVPGIALESSSGPSSDASERLNQL
                     GFFTCEVWTVGEQYIAAYHVDCNAGSYTAQKTPGSLWNQAILGYGPRDEVSDAVRKGV
                     RAMVELFANTFANARMDAGR"
     gene            51520..51999
                     /locus_tag="PK224_01035"
     CDS             51520..51999
                     /locus_tag="PK224_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14356.1"
                     /translation="MADASSGRRISHPYGWLLLGLLLLFFFRVAAQLIQAYSPVTSLP
                     PFETWQSGALPYPLLVSLQLIILVACGRVVWSFLSGTVVPSGKKGRLLLVLGWVYFVV
                     MGIRLLIGLTVAPNHFWFGAIVPTLFHLVLASFLLVSGSFHTASGSCSVAPVSEGAA"
     gene            51996..52871
                     /locus_tag="PK224_01040"
     CDS             51996..52871
                     /locus_tag="PK224_01040"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRB14357.1"
                     /translation="MILLLRYGAYPSILLACVIGNLVLFHMGTGVLPATYLPVTLGTL
                     LIMALEATLPYRSDWRPSRVEVIQDSTFLALVHIILPKALAAASVVVIFDVLHGRGWG
                     MSVWWPHDWPVPTQTMLMVLIVDGLRYWLHRLSHEWEPLWRVHAVHHAPQRLYTLNVG
                     RFHPIDKGLQFVFDALPFLALGVQEDVLSLYFVWYAVNGFFQHSNADVRLGWLNYVVS
                     GPELHRWHHSMQKEESNHNYGNHLIVWDVVFGSRYLPADGSVGALGLVNRQYPRGFLD
                     QMAAPFTPGLDKQTV"
     gene            52880..54517
                     /locus_tag="PK224_01045"
     CDS             52880..54517
                     /locus_tag="PK224_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRB14358.1"
                     /translation="MLRFLHDPALSLSLTLTKYRDWNPFIAHTHCPELAQQAALRDIL
                     ARQAGTAFGRKHRFGSLRRYEEFACEVPVCTYEDLRPAIEVQEKSREPLLTSGQPILF
                     AQTSGTTGVPKNIPILPQTVEAIRRYQGLCAYAQWQGVPAIYQGRVLVISGQTIEGHL
                     PGGTPFGSMSGLMFDCLPAAIRRKSILRGGESAASDYRQRYFNIAVRALADSSLSVLA
                     TPNPSTILKLLEVIRGEYALLLEALSGETRGGCLPPFEKLPISSTRLSQLRVFIGQEE
                     ALDCATLWPNLRAVVTWTGGNCAVLIPKLGSLLSPQTRIIEMGYLSSECLGTVNVDVL
                     NNRCVPTLADNLFEFVEVGDEASDVEPVLLQQLQAGRKYTVIVTTRNGLYRYAMNDII
                     EVTGYFNRTPTIRFVQKGKGVTNITGEKLYEHQVTEAVGQVLIARGLSSEFFVMLADV
                     ENSRYTLCLEHASSLGDLGILVEERLSSMNIEFKAKRASGRLQPIHVLQLRPGTGDVY
                     RQHCVSKGQREAQFKLIRLQYAHDSTFDFERHGEVMT"
     gene            54517..55611
                     /locus_tag="PK224_01050"
     CDS             54517..55611
                     /locus_tag="PK224_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRB14359.1"
                     /translation="MRMRSLTMTSLEIPFRQVFTHASATRAKTEAVLVRAESARGLVG
                     MGEGCPRQYVTGETVASAQEFFRSHRAEWMTCSSMDDLQTWGTAHADLIDRNPAAWCA
                     VETACLDLLGKELEQPIEVVLGGTPLSGPFRYSAVLGGEKFSSFEKQLRQYLAAGFID
                     FKLKVMGDLEADRERVAALTAAGTPGLRVRLDANNRWHRVDEACEYIQSLQGSFTALE
                     EPLRVGDYEGCRALSRHCGVPIILDESFVNVGQFPLIEGEPSLWILNIRVSKMGGLLR
                     ACTVAARAKAAGIPIVVGAQVGETSILTRVALVVADRYRDILIAQEGAVGTHLLEYDL
                     CEPPLMFGRGGCLADTVFYGRSGLGLSFAR"
     gene            complement(55678..56706)
                     /locus_tag="PK224_01055"
     CDS             complement(55678..56706)
                     /locus_tag="PK224_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRB14360.1"
                     /translation="MENWLSLKRRNLLKLTGIAAVAGLTGGCDAVGSLFGRMFAMPPR
                     ETNYFTENKKFYVVNYSDSPFNVSRELRQDEWRLSVKGEVKKPLSLGWRELLNRNNFE
                     QISTLMCIDTLPGGDSLGNARWRGISLKKLLQEAEIDEETTRDIVFRGADAYDDSIPL
                     TRAMQDDVMLAFLMNGEKLPKEHGFPLRLLVPGLYGIKNVKWIVEIEAYAGDYRGYWQ
                     RKGWTDDATIKTFSRIDSPGHYQTLRGPEQRFRGIAFGGPHSISRVEISLDATRTWDS
                     CEIETPMSPYSWVIWNYTWRPSKRGKYQVAVRAIDSKGQPQIAELIRPQPAGSSGLHT
                     IVADVESL"
     gene            57028..58566
                     /locus_tag="PK224_01060"
     rRNA            57028..58566
                     /locus_tag="PK224_01060"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            58738..58814
                     /locus_tag="PK224_01065"
     tRNA            58738..58814
                     /locus_tag="PK224_01065"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58772..58774,aa:Ile,seq:gat)
     gene            58879..58954
                     /locus_tag="PK224_01070"
     tRNA            58879..58954
                     /locus_tag="PK224_01070"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58912..58914,aa:Ala,seq:tgc)
     gene            59075..62081
                     /locus_tag="PK224_01075"
     rRNA            59075..62081
                     /locus_tag="PK224_01075"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            62208..62324
                     /gene="rrf"
                     /locus_tag="PK224_01080"
     rRNA            62208..62324
                     /gene="rrf"
                     /locus_tag="PK224_01080"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            62524..63204
                     /locus_tag="PK224_01085"
     CDS             62524..63204
                     /locus_tag="PK224_01085"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRB14361.1"
                     /translation="MLPPIPSSWYQFFTEQINGNNFRALETFLDREAEAGETILPPSQ
                     DIFGALEATPYEQVNVLLLGQDPYHTPGMAHGLCFSVRPDVPVLPRSLRNIYRELRED
                     VGCSVPNNGCLEPWARQGVLMLNAVLTVRAHAANSHRGRGWEAVTDRIFAVLNAKPSR
                     VVFLLWGAEAKKKQSLITNAQHVVISCAHPSPLSARKFFGSRCFSQVNRALTEVHLKP
                     IDWQIPDL"
     gene            63303..64091
                     /locus_tag="PK224_01090"
     CDS             63303..64091
                     /locus_tag="PK224_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HRB14362.1"
                     /translation="MRIRLGLGLGFSTLVVAGTLLAAEGDLAGLRTPTSIGSAPVAWQ
                     YDGDQGPSHWGEIAPTTASCEKGTHQSPINIRTTPHHQSHDGLLVHYTAAPGHVVTSH
                     HTIEVDFQSGESLEVLGRTYTLKEFHFHEPSEHQLNGRTYPMEAHLVHRDETGHLVVL
                     AVLMDLGNESASLSAVWDRIPSEKQDEVRDLLINPQDLLPKDLHHYAYDGSLTTPPCT
                     EGVHWIVLKEPTSITSAHIERFVSLIGHNARPVQSLNEREIDEE"
     gene            complement(64459..64845)
                     /locus_tag="PK224_01095"
     CDS             complement(64459..64845)
                     /locus_tag="PK224_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14363.1"
                     /translation="MSNGQDSQTSPVARWVRIPNGTKVRHRHKGYDGHIDGLTEIVVG
                     PNRNPDGKTQYRLNIGGSSRELVTEDDLSILVDGDNLVIMIRQKEPYRRSITAQLHGV
                     LAEDRFIKAGRSSAVPATTPPQVPSA"
     gene            65715..65852
                     /locus_tag="PK224_01100"
     CDS             65715..65852
                     /locus_tag="PK224_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14364.1"
                     /translation="MMAQTVLFWAAHNLFTVATVCFGGAMLTVMLYTYLHDYVLVTKG
                     R"
     gene            65900..66187
                     /locus_tag="PK224_01105"
     CDS             65900..66187
                     /locus_tag="PK224_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14365.1"
                     /translation="MRIHRTITTRVLSLFLATAWLLAVSLTVHAAEQKPVVTVAPVKS
                     VAVASGAVEDTLPTCLTRIPQGATPGQRLIAEQSCQRDESDRASMRAVPGR"
     gene            66326..66622
                     /locus_tag="PK224_01110"
     CDS             66326..66622
                     /locus_tag="PK224_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRB14366.1"
                     /translation="MRMEHRVRSRSAGRPGVVEERMVSGAGETPAGVGRNMLLDINRA
                     EISRLKSLTGIGEHYAKKIVEGRPYHHREELLTKEILPEYIYGRIMDRLMASQS"
     gene            66820..67428
                     /locus_tag="PK224_01115"
     CDS             66820..67428
                     /locus_tag="PK224_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB14367.1"
                     /translation="MALVAAISLLMGIVQATAAWSEQGPTEAVKGTVSELLSILKELK
                     DPTRSEARRWEIEQVIRHHVHYEDMAKRSLGASWGQLSDVARREYVGLFVQLLRDALA
                     NRMVEYSGERISYLSEQRERTFAQVKTRLVGNKVDTFIDFRLVSQDGRWLVYDAVMDG
                     GSLVASYHAQFASIIRDASCAQLMERIKEKTLVVKLFEKSGS"
     gene            67484..67672
                     /locus_tag="PK224_01120"
     CDS             67484..67672
                     /locus_tag="PK224_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14368.1"
                     /translation="MRCPRCYGPMMMIKMKDIVSGTLAYGWGCRQCGAVIDSLIDIHR
                     QWHLEPRATWAHLAVAAC"
     gene            67775..67942
                     /locus_tag="PK224_01125"
     CDS             67775..67942
                     /locus_tag="PK224_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14369.1"
                     /translation="MLHAALIALVLGLVVWGLNWTGLAEMPTQVYWVFTVGGMLLMLI
                     HVATRRPARVW"
     gene            67990..68547
                     /locus_tag="PK224_01130"
     CDS             67990..68547
                     /locus_tag="PK224_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phycobilisome rod-core linker polypeptide"
                     /protein_id="HRB14370.1"
                     /translation="MLGISAPCFSEVEAAKEWAPQEVTAILAQSYGQLLMRPMGMEPS
                     SLQKNEADQQRYMVQSGYALQLAKGEKSVKQIVEAIALSSEFNAKWITPHMGTAQTGS
                     APGVPPVAAPEKAIDSLYCALLGRRVDSVSLASARKDLVSSGFERVIRDVIDGKEYRD
                     RFGDAKVPQVSSEKQAAVGCPQVAL"
     gene            68645..68827
                     /locus_tag="PK224_01135"
     CDS             68645..68827
                     /locus_tag="PK224_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14371.1"
                     /translation="MTPEERIAIMTKAINQAIKVMGDRFGSTEEDVARAIQGLKASMQ
                     TAPQEKPVPVGAATQA"
     gene            69256..69576
                     /gene="erpA"
                     /locus_tag="PK224_01140"
     CDS             69256..69576
                     /gene="erpA"
                     /locus_tag="PK224_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRB14372.1"
                     /translation="MITITATAEEKIRELMLEEKDTLGLRVYVKGGGCHGYQYGMAFE
                     SKMSDDDTVIEKGDVKVIMDSQSAPLLSGCEVDYVDSVQGSGFAIKNPQAKTTCGCGS
                     SFSA"
     gene            69634..70839
                     /locus_tag="PK224_01145"
     CDS             69634..70839
                     /locus_tag="PK224_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB14373.1"
                     /translation="MEPTSVIGTKNKKGQVFTDPRIMMREAPRTPSFYTGSEVIKEAI
                     RRASIDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGARV
                     FTTTAGPGTMRAMENFPMWAGARLPIQCIVTCRGINSPLSIQPDTIEISYLMQTGMLV
                     WHAETAQDFYDWILMGYMVSEEPEVHLPLALCCDGFFVTHTKDVVDLTPTDMCLPPYD
                     PYRSPVPCMDMECPPVRMMRDPFIMKSNYISYATHASWQQEVWAAAERSRKHTIAWLN
                     GLIETEDVDADVLIVTSGTAVSQGREAIRLLADEGIRVGLVKIKTLRPWPEEEIKEAT
                     KHATHIIVPEFNVIGWMAKEIKATIPRSERVIAGPRVCGGMTMPPEIIVEEIKRALGV
                     RSGTLAGRG"
     gene            70922..71818
                     /locus_tag="PK224_01150"
     CDS             70922..71818
                     /locus_tag="PK224_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB14374.1"
                     /translation="MSKERIKISPDLYDIMPSDYQDLVSSATYGKEDRGWKDIGTSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPDRIKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVNCTVSKPSLVEKV
                     VRNAVLIAREIGPTYLQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAFKEYISEPAK
                     QLLAERDAKAKEKKAAAKQLVS"
     gene            71871..72764
                     /locus_tag="PK224_01155"
     CDS             71871..72764
                     /locus_tag="PK224_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB14375.1"
                     /translation="MSLDYVKFTPGFEQFMPKEYRDMVEHGPFGKKTTVSQMGSFKEI
                     LEEHPMCAGCAMTLFIRLAIIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVIAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTTRGAVLKMAPLGKKFEKMDMVTMAKVAGCAYIATVVPNNPRRVESVI
                     KKAVLIARDIGPTYIQAYTSCNIEYAIPTDKVMEDAKTVEDDRYKFTEYVSDEAKAYL
                     AERYGYKEFMPKAVPASVTTA"
     gene            73208..73450
                     /locus_tag="PK224_01160"
     CDS             73208..73450
                     /locus_tag="PK224_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14376.1"
                     /translation="MTQEQFQQFWLQLKAPLKANWDKITESDLGEIQGSLVKFGDVLQ
                     KRYGEGHKDEVSLWADRRHAHWSGNYIGYKDPKPAV"
     gene            73541..73885
                     /locus_tag="PK224_01165"
     CDS             73541..73885
                     /locus_tag="PK224_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14377.1"
                     /translation="MRKIVINKSYDQFCLSHKAFLRLRELGQPDALKETDLGAYWPSA
                     AGPQEPSLNQCGMLIPRDDKRLVQVVEELQGEANGHCAQLKVVSIPDDVSWVIAKANG
                     SEHVSEQHRTWS"
     gene            complement(74249..75841)
                     /locus_tag="PK224_01170"
     CDS             complement(74249..75841)
                     /locus_tag="PK224_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB14378.1"
                     /translation="MMILILMAMAVPPGSVQAQEADAEVLVAEGILAYEAKRYDEAAA
                     LFSRAVATDPRNARAFYYLALCHLARGQAELAIAPLTALHTLRPTDPEATYQLGVAHF
                     ATKHYDKAAPLLEDVFRLEPDRENLGFYVGFLRYRQKNYNGAEAALSVSRSADQDIRQ
                     LTMFYRGLALGILGLSDQAQAELASAQRMQPTSPITGASVRIQEALTATTRVTDPQRL
                     RMQLAVGGYYDDNVAVNPRKSSDQVAELLRSRNTQSPGFVTSARGDYAWYRKGPIEST
                     VTYSYYQTVNTDSGAGSFNIQDHLGGLSGVYRGTVGTMPYEIGGQYSYDYMLLGLKGF
                     LSRHTVTLPATFVPPAITLPALGRMDNLTTLLYRFQRKSFFTEPGNSDIRFAPESRDA
                     FNNMIGVLHAFRFRQDTIIVRVGYQHDTEAASGSSFAYSGNRLQLGGQTTLPWYDLSL
                     RVDYEVHWRAYNHAQVIFLDDAGQLSRRRDTEQDIFVQITRPLFRNVTVALQYQGVLN
                     GSNVPVYAYDKNVFTTLLTWTY"
     gene            76189..77217
                     /locus_tag="PK224_01175"
     CDS             76189..77217
                     /locus_tag="PK224_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14379.1"
                     /translation="MSTMAWAEEPPLPLSGEGQARGVVLGIVGMPVRIVAPAATPGGT
                     LNVGDVISAGDELRVGTGEQVALLWDHRAVLTLREGARMNIQESHRGQTELLLHQGMV
                     RIALSYNAGRMTDTLTLQTPLARVVSRGGIMEATVLGGERQSLLARLITAPPGDTFRV
                     LEGQARVEPLTGDRKAFSLKVGTEFALRTGMVTSLSELQEGSHVPQPLAVREEHRALP
                     SPVTRQIVGTQVGHALELEKEIRQASSAGGEKELPGPSTKGTILATSIGLPVVTFSQT
                     PSQVSVSGTSTGVPTVLPPPAVPPVQSAGVGSLGPAQSGGLNSSRLLQEILTEVGKIG
                     KGHGKKDR"
     gene            complement(77238..77657)
                     /locus_tag="PK224_01180"
     CDS             complement(77238..77657)
                     /locus_tag="PK224_01180"
                     /inference="COORDINATES: protein motif:HMM:NF023520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HRB14380.1"
                     /translation="MRRLIPIVLLLSELIQTAGCLSARAPACLPGEHPAVLESLYFGT
                     AKPGGIVSPEEWTAFVKDIVTPAFPEGLTSWQASGQWRKSTGAIEYETSRILQLTHEQ
                     NMAKEAAIQRIMITYQHTFQQDAVMRVRSPACLSFKG"
     gene            complement(77681..77968)
                     /locus_tag="PK224_01185"
     CDS             complement(77681..77968)
                     /locus_tag="PK224_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14381.1"
                     /translation="MRTTLIAFALSLSSFLFTLSSHAAAENSGTAKSGSDQNLTLDDI
                     GRGLKSAAKNIEEEIPKIGPAIGETFKKVTGGGKEKSPPKPSVQNTTKDKK"
     gene            78263..79558
                     /locus_tag="PK224_01190"
     CDS             78263..79558
                     /locus_tag="PK224_01190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB14382.1"
                     /translation="MQTTAFTSFDSLGVSPTLLRNLTKAGFAEPTAIQAQAIPHALAG
                     RDVLGCAQTGTGKTAAFVIPMLERLSGTPKGQPRALILAPTRELAIQIQATIDTLGRD
                     LQLFATTVVGGADMQAQVRGLRQRPDIIVATPGRLLDHMWNGTISLLAMSILVLDEAD
                     RMLDMGFAQQINQILDAMPEERQTLLFSATMPNDLARLAQASVKDPVRVMVTKSATTA
                     DGVTQAVHHTTHDRKNGLLMSLLQSESDTVLVFARTKHRADRLGNLLDSAGHRVAVLH
                     GGRTLPQRRAALEGFRRGTYRVLVATDIAARGIDVANIAHVINYDVPNCPEDYVHRIG
                     RTARMRTTGRATTFVTSEDQEQLRAIERLLGQAVPRAEGSPASMSASPRPDGHAPRSE
                     PERRRRRGGSSQVWSQSADGGPREANGGIKNGSDLGPKS"
     gene            79588..79935
                     /locus_tag="PK224_01195"
     CDS             79588..79935
                     /locus_tag="PK224_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14383.1"
                     /translation="MKVSDQNQDAPRAASQWVRIPDGTMVQHRLDGQKGHIDGLTEIV
                     NGPSRNPDGRTQYRINVGKGDRVLIAEEDLLILTDAEGLVRMAKEKGEYRSLVSKQLR
                     GVFGEDRFVIKAS"
     gene            complement(80217..80498)
                     /locus_tag="PK224_01200"
     CDS             complement(80217..80498)
                     /locus_tag="PK224_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14384.1"
                     /translation="MKITSVWRGNKPNHKKKRTVPKLRIRWTQLGLVDPSVDSPETPM
                     DEIRRAVTNLASSGFGAETRARSEQTQARPARAHARTSATRTTRRPSGS"
     gene            complement(80869..82422)
                     /locus_tag="PK224_01205"
     CDS             complement(80869..82422)
                     /locus_tag="PK224_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB14385.1"
                     /translation="MQRATLNFETLLEVTNALNSQRDIDSLWRVIADQIRKVIPWDRA
                     GITLYESQTDSFRFYAVITNMATPALAHDSVIPRKGSAVGWVHDNRRLHIREDLQKEQ
                     VFLEDRYYVQEGLGRMINLPLLVREHCLGTLNIGSVQTGHLDQDDCKFLQQVATQIAY
                     AIDHVLAYEQIQQLSDRLRRENEYLAEEVKASRNLRLVVGTSLSFRKVVDLLKAVAPT
                     DTTVLLLGETGTGKEVLAQALHDLSLRSHKPFIRVNCAALPSGLIESELFGHERGAFT
                     GAQLRRAGRFELAHTGTLFLDEIGEMPLETQAKLLRVLQDGMVDRIGGTQPVAVDVRL
                     VAATNADLQAAVRQGTFRADLFYRLHIFPIALPPLRERREDIHLLAQHFLAQIGGKLK
                     RAHLAFDPQSMARLLTYNWPGNVRELQNVIERAVILSGSSQVTVDEMLLPVVQAVHSP
                     SSSQPVSLGDLERHHIIEVLERTKWRIYGEQGAAHLLGLNPETLRSRLRKLGLRRPAG
                     ISPEPTALP"
     gene            complement(82429..84132)
                     /locus_tag="PK224_01210"
     CDS             complement(82429..84132)
                     /locus_tag="PK224_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRB14386.1"
                     /translation="MLRSTLTRTVLLVCVILLESTGATLVLPPVWAESERAGYGPASP
                     GLSSSPVRLRFEPDDAVSSPLGSSFDLQHVWMRADLRVRPEWRNSVCFGGGPPIAGAC
                     NSLDQFGSGTGAHSGRHAGDFFIQQWARIGLGYDPSPNVNFYVELQDSATWGGNGDPT
                     GRNQVGDASTHNCGIQLAGQCRLGIRAGYMLVRNIAGIDGFSAKIGRQYVVFGNQRLF
                     GHFDWANTGYSHDGIMLSYATAAFDTKLGWFRNSETDLGQASPGGSLTPNLLTCNPEA
                     NSSATCNPALAQHATDAGSDVDLVVFYSQLRSIPRMIVEPYYILYSNRLPERANPGQY
                     LPKSASQLRHMVGLRIEFRDGNWDFTHEAAYQFGRSADGFDFDNRRNLRINAWASGTW
                     LGYTWYTHHWKPRVAIGFDYASGDGDSNCVPPGGNLARSCGGNANTFENFFPTNFLHV
                     GYMLNGAWRNSVQPQVNVQARPTARDHIELWALRKYLASARDNWYRGSQGPLIFSASD
                     NRATHIGDELDIAWSHMFADGTVSLSIIYGHFFSGSYIKHQLGTAADQDWGIVQLWTN
                     F"
     gene            complement(84248..84739)
                     /locus_tag="PK224_01215"
     CDS             complement(84248..84739)
                     /locus_tag="PK224_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14387.1"
                     /translation="MKRHIATYSAIVLACSWALLGPAPTQAAADNIEPVTWSNSQKSS
                     AWAEELLGQVVTYQTLAEKSLIPGNFEAYVEQMRKVRELYRTGNRRATYDGVNQLMVM
                     LEARVGGIDAHSADALWDFCYRVTPDEYHARDRHIRAKGADEVKKHEDFMRDMEERAS
                     MAF"
     gene            85270..85995
                     /locus_tag="PK224_01220"
     CDS             85270..85995
                     /locus_tag="PK224_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRB14388.1"
                     /translation="MDKALYPYLLFWSIGLLCFLAEWRFPARPIAYRSVFWRDLIALG
                     LYNVSFLAVVQATDRIPIPNYMPAVLYNLPTVVKLALFYIVEDFGLYWVHRLMHTKPV
                     WRIHRWHHSPTSLYWLSGIRATIPHIALFNLTYIIALPLLHEASAWAFQVIMVEHIVR
                     NNWMHLNVTWRSSWLEWVFVTPRYHQIHHSTDPAHQRTNLGALLTIWDRLFGTYYNPD
                     DVRGELSFGLAERVPPVRLVMGL"
     gene            86132..87226
                     /locus_tag="PK224_01225"
     CDS             86132..87226
                     /locus_tag="PK224_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB14389.1"
                     /translation="MQGPVLSIIVPTFNERDNIPRLVAALDACLVGVEWELIVVDDDS
                     PDQTAAVVRAQACANHRVRCLHRIGRRGLSSACIEGMLSSSAPVVAVMDGDLQHDERL
                     LPDMLKVLLQGGYDIVVGSRYMAGGSIGEWDGLRALTSRWATRLSRPLVPAGLTDPMS
                     GFFAMRREVFERLVRRTSGMGFKLLLDVLASSPHPLRFIELPYEFRSRQVGESKLDSQ
                     VAWDYLMLLLDKSIGRFVPVRLVTFCFVGGSGVLIHFSLLWVLYRWLAVPFAWGQGIA
                     TLLAMTSNFILNNIITYRDQRLRGWQWLRGWLSFVLVCSLGAVGNVGVASYLFTQQFH
                     WGLSALAGILVGTVWNYAVTSSYTWKVKAR"
     gene            complement(88931..90181)
                     /locus_tag="PK224_01230"
     CDS             complement(88931..90181)
                     /locus_tag="PK224_01230"
                     /inference="COORDINATES: protein motif:HMM:NF023301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HRB14390.1"
                     /translation="MSLPRMPLLLLSLSIPALFSGCETTDKMLGAAERAVGSSTGRTV
                     LDIVGGKDPAEIARRRVENYGRDPQALLRDLRSIQRDFQALMAALTGEVGKKWGTKEV
                     KLPEQKKYVKYTQNYRSRAIVDFDAGNILVETLDEKDPRASLKNAVVTTLLTPNDPRS
                     VDLFTDKEITLTSDKEPYLLGLVLDQAGKPVRTPAEAEQFADTLLSKSTTTRKVEQEE
                     GPKTAHMVNISMVTNFSHKQAEKYRAVVGQFAERYQISPSLVFAIIRTESNFNPFAVS
                     SAPAYGLMQLVPTSGGRDAYRKAKGEDKAPSRDYLFDPDNNIELGTAYLNVLTYAQLD
                     DVTDLVSREYCVISAYNTGAGNVFKTFSKDQRNALQQINGLQPAALYDRLRTGLPYQE
                     TRDYLAKVVGFRKQFVSLGDTAAK"
     gene            90384..91034
                     /locus_tag="PK224_01235"
     CDS             90384..91034
                     /locus_tag="PK224_01235"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB14391.1"
                     /translation="MNHRFVSLSHSCPSTLRSVRGLIVCLLAVMLVAVSGHATSSWAG
                     QTPTEVVERTMKDMLYILTELKDASRSAQRQWEIEQVVRRVFNYEEMAERSLGETGIK
                     SNAVERRQFVRLFVQVLRDDLADHLRDYSAAQVVYLAEGNDADGAQVMIAPAGQEVDT
                     RIEFQVVRRSGAWLVNDISIDGASIMAKYQAQFTRILREGSFSDLMEYLKQKAVIA"
     gene            complement(91181..91930)
                     /locus_tag="PK224_01240"
     CDS             complement(91181..91930)
                     /locus_tag="PK224_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB14392.1"
                     /translation="MIHERGSGSALITIAIVSQNQLLRLGLQAVIAPHAHIRLIGEAT
                     NMLQAVSIVTQEKPQLLIIELQPGMDILGLVRKVKASLSTIRIIALHGIEDSRAGLEA
                     LSSGIDGMVLTTQPGVVLLAVIEYLCRRPANVARCHPCDTSALETTGSAGGFEDRIPV
                     LPNWSEDLTKREHDIITLIGEGLSNKDIACRLSICSTTVRHHLTSIFGKLDVHSRQQL
                     LIRAHKYGLVKLHANGTAARAHLVAGSEQSA"
     gene            complement(91952..92242)
                     /locus_tag="PK224_01245"
     CDS             complement(91952..92242)
                     /locus_tag="PK224_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14393.1"
                     /translation="MRAREGLQLLFSGQHEYVFSYGPSLSYLKVVLSTVALNLFSTHG
                     LEPVRLATHVAEWALLMGQVCGTVRPSGDLVLSSCYEYCRRQAVTHSGVSPR"
     gene            complement(92397..92723)
                     /locus_tag="PK224_01250"
     CDS             complement(92397..92723)
                     /locus_tag="PK224_01250"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB14394.1"
                     /translation="MAQRRLRSIEDSVRIPSRSTKEMETSARQRSGPESPPQQADNEA
                     TTEEIRTRIEKLAYELYQQRGRQDGHDRQDWLEAERLTLTEPTRARHDRSDRLPTSVQ
                     ASRVGR"
     gene            complement(92779..93210)
                     /locus_tag="PK224_01255"
     CDS             complement(92779..93210)
                     /locus_tag="PK224_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14395.1"
                     /translation="MSATMNSTKQTGRSRVMIVDPEWQFGLKLADCLATNGYHAVLVR
                     DLQSMLTELGEIQPEAILLSSDSCDEKRGTAELDTLRALNVLCPQAPILTVVEPMRDA
                     LGTLSPPIDARPRHQAPLVHNRVEELLRANLGIPCVRASLT"
     gene            complement(93226..93513)
                     /locus_tag="PK224_01260"
     CDS             complement(93226..93513)
                     /locus_tag="PK224_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14396.1"
                     /translation="MTELYETEALIIRELEQQGPCTLDALAYRFPACTWNQIFIAVDA
                     LSREGTISLQPRAGCQYLVSLASVHAHTLHHFDPDRRVAETRQHTGRSYGR"
     gene            complement(93786..94430)
                     /locus_tag="PK224_01265"
     CDS             complement(93786..94430)
                     /locus_tag="PK224_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB14397.1"
                     /translation="MTRILVVDDHAVVRQGVKQILSEQFHGAVLGDAQNAEEMIAQVG
                     KHSWDIVILDVGMPGKSGLDALKELKQARPKLPVLVLSGYPEDQLALRMLKAGAAGYL
                     SKDSAPNELVQALRKILGGGKFVSAAVAELLALNLESDWEKPLHEQLSDREYQVMCLI
                     AGGKSLKDIADDLCVGVSTINTYRARILEKMKLKNNTELTHYAIEHRLVNRLIR"
     gene            complement(94509..97505)
                     /locus_tag="PK224_01270"
     CDS             complement(94509..97505)
                     /locus_tag="PK224_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB14398.1"
                     /translation="MSTGPAHILLVEDNAGDARLLKELLTETGAGRLTLTHVDRLDAA
                     IRCLTQAAFDIILLDLSLPDSQGAETLVRMHAAAQGIPIVLVTGLEDEELGIQLIQAG
                     AQDYLVKTQTTAPLLARALKYAVERKRLEEELRLKTRLLQSVLDNMAEGVVMADERGT
                     FQVWNQAAERIIGSGPADIGIGEWSKHYALFLPDRVTPFPTDDLPLARAIRGESVTDA
                     LIFFHRPHWPGEAWLSVTARPLMDDTGHLSGGVAVFRDITAAKRTDEALRDSQERYRL
                     LVTKANDIIYRTDASGRFTFVNPVAMRIMKYTEPELLGRRFIELIHPDHRPAAERFYG
                     RQFIRQQPSTYYEFLSLAKDRSEVWIGQNVQVILEGGKVVGFQAVARDITERKRAEEA
                     LAESEGRLRSIVQSTSDAIILIDTQGRVAFWNRGAEKIFGYGAEEMVGQPMTRIIPER
                     FREAHERGVQRVAAARRLTMHAAMFELRGLKKNGTEFPLELSLATWAIKSQQFITGIV
                     RDISERKLAEAALRDSEERFRAIMDHSPALIFIKDLEGRYLQANRQFEIVSHLTHDDL
                     KGKTDEEIFPPEQAAVFRANDRKVLDSGAPMEFEEVVMHDDGLHTSIVVKFPLRNAQN
                     RCYALCGIATDITDRKRAEEARQQLAKDRLLLLESSGEGIYGINRQGRCTFINSAAAR
                     MLGYLPDELLGQDMHERIHYSFTGGTAYPRAACRIQETLERGTGCKVDDEVYWRKDGT
                     DFPVEYSSFPIIEQEQIIGAVVVFLDTTARKRAEQQLTLSHDQLRKLTARLESVREEE
                     RILIAREIHDELGQALTGVKLDLCLLRDQISEVSPAMMKRLESISTLVDGTIQSVQRI
                     ATELRPVVLDQLGLIPAIEWQAHEFQARTGIQCTLDVYLRTITLSHAGSTAMFRIFQE
                     ILTNVARHAQASIVHITLQEQAGSLVLDVRDNGRGVTDAELADPHSLGLVGMRERALL
                     LGGETIVTGSPGTGTAVRVRIPLGQP"
     gene            complement(97502..97963)
                     /locus_tag="PK224_01275"
     CDS             complement(97502..97963)
                     /locus_tag="PK224_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB14399.1"
                     /translation="MVPEMVKPIEILLVEDNPGDVRLTMEALKEAKVVNHLTVLKDGE
                     EALTYLRRQGPYAHAMRPHLILLDLNLPRKDGREVLAQIKAEEPLKRIPVVVLTTSED
                     EQDVLKSYNLHANCYITKPVDLDQFIRVVRSIEDFWLGIVVLPVPPQNGTR"
     gene            complement(97987..99759)
                     /locus_tag="PK224_01280"
     CDS             complement(97987..99759)
                     /locus_tag="PK224_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14400.1"
                     /translation="MEPALNNAPSKAGLVSDRSRSTQGGLPLVLLLCFVIATALGLYA
                     LSFLRTVLVSERGRELAMNAAAVADALDRVMFERFGDIQLFANDGILRDGTSAEKTAR
                     LLAYKQLYQYYSWLGVADENGHLTAATDALPAQGTQGLNPESFELVRRTGKVHFEDMR
                     PSPASQRKMAIGFTAPIYGPKGEFHGVVVTRVPFENLQSIFEQEGRLRYGELAYDWLL
                     LDREGAVLSEKHRPSNLNGNPVKIDLPSQAQAVIHRDHPGFVQETHRRGGNAVVTGYA
                     WTRGYANFPGFDWLVLFRLDRDQVYAPIDRLIWMVGGIGLLVILPLTGYGIWVSWELG
                     REKNDLVEARQKLEESVSELGRSNADLQQFAYVASHDLQEPLRMVSSYTQLIARRYKG
                     KLDADADEFIAFAVDGANRMQRLILDLLAYSRVNTAGRQFEPTAMETVLKAALNNLTD
                     AVKESQAIITHDPLPAVMGDDKQLAQLFQNLLSNAVKFGGAQPPRIHISAKQTDGEWL
                     FSVRDHGIGLDPQYADRIFVIFQRLHTREEYPGTGIGLAICKKIVERHGGRIWVESEL
                     GKGATFYFTMRDEEPGTSAHPPNR"
     gene            99958..100374
                     /gene="arfB"
                     /locus_tag="PK224_01285"
     CDS             99958..100374
                     /gene="arfB"
                     /locus_tag="PK224_01285"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRB14401.1"
                     /translation="MLSISTHVTIPDEEIELTAVRAQGAGGQHVNKVSSAIHLRFNIL
                     ASSLPPFYKERLLALRDHRISRDGTIIIKAQDSRSQERNRALALDRLQELIQSVAVLR
                     PPRRPTKPTKGSTQRRLDSKTKRARLKSLRGKPDEQ"
     gene            complement(100455..100778)
                     /locus_tag="PK224_01290"
     CDS             complement(100455..100778)
                     /locus_tag="PK224_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRB14402.1"
                     /translation="MGTKLYVGGLPYAATESQLSNLFSAHGTVESARVITDKFTGQSR
                     GFGFVEMSTAEEAKAAITALNGSDMDGRQLTVNEAKPQEARSGGGGGGGRFGGGENRG
                     GRSRF"
     gene            complement(101144..102013)
                     /locus_tag="PK224_01295"
     CDS             complement(101144..102013)
                     /locus_tag="PK224_01295"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRB14403.1"
                     /translation="MQQIAPSSVPTDIVHRVHHKFWTYLLFWSVVAGSMIAVPGYGLV
                     YGYSWLDWTMFAILYVVTGLGITVGYHRLLTHRSFECPDWVKGCLLVAGGWALQNSGA
                     KWTADHLRHHAHCDDPADPYNAKRGFWYSHCGWLLDPVPCNDERFGSRVMQDRVAMWQ
                     HRYYAVIVLVGLALPFVVGFLHNGWKGGIGGFMLAGVGRTFAVLNSTFCINSVCHLWG
                     EQPYGTADSSRNSWFVSLLTFGEGYHNYHHTYPSDYRNGPLWYNFDPSKWLIYTLWKL
                     GLASSLRTASSGH"
     gene            102256..102612
                     /locus_tag="PK224_01300"
     CDS             102256..102612
                     /locus_tag="PK224_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HRB14404.1"
                     /translation="MRVDCGVLRKAMGIVVLSGLAVELFGFYEPAWSTSDPATAAGQD
                     QSAAPPESSGSPGAGAKEKGFGGHRMRKVCAEDVKKLCPGVKAGEGRIAQCLKAHAAE
                     LSQTCSEMIRQRGQHR"
     gene            102785..103165
                     /locus_tag="PK224_01305"
     CDS             102785..103165
                     /locus_tag="PK224_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB14405.1"
                     /translation="MDGTTGQGQFSVDLRQHPRLRVSAPFVCSFSRLGLAKWLGRGGD
                     GIGVVFDVSMRGAKVMSESGIQRGDRVSVTLALPNQVSPMTVEEATVRWEKEQVYGLE
                     FVDLSPVAEMRLRKFITIATKPGA"
     gene            103368..104306
                     /gene="lpxL"
                     /locus_tag="PK224_01310"
     CDS             103368..104306
                     /gene="lpxL"
                     /locus_tag="PK224_01310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HRB14406.1"
                     /translation="MSSTNTRKKRQRPHSSYHPLYWPTWVGLGLFRLLALFPYRWQLA
                     FGRQCGRLLHGLLTKRRDIVRVNLAVCFPRQEPVERNQVALHCFESMGMGVLEIAMAW
                     WAKDPRTYCECTIKGLEHIREALRQGRGVLLCGAHLHAAELAGRFMALEQPVAIVYRP
                     QNDVVAETVANRCRSRYYSELIQHRDMRGILRALARNQVVWYAPDIDAGSKRSVFAPF
                     FGVQAASLTATSRLAKVSGAAVVPCFYYRRADGSGYDIEVGPALEHFPSGEMVEDATR
                     INRLIEGAVRRVPEQYFWQHRRFKSRPPGEPPIYRR"
     gene            104426..104827
                     /locus_tag="PK224_01315"
     CDS             104426..104827
                     /locus_tag="PK224_01315"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB14407.1"
                     /translation="MHDRTIRSAMDQSNNLSSARIELRQHQRCVVPPACLLSFAPFAP
                     TISFNGDAEGEGVVINLSAGGCKISSDAGVTVGDAMSLIILLPGELCPTTIDLALVRW
                     GRDLHFGVEFVSMGTTEFNRIRQFLASTGPE"
     gene            104882..105214
                     /locus_tag="PK224_01320"
     CDS             104882..105214
                     /locus_tag="PK224_01320"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB14408.1"
                     /translation="MEQTKTPRKSRRVATDCRLEFTGEDEVDGEAEVLDVSSTGCMAK
                     SDNRMSPGLHMKVALFLSDGHEWPVRVDEAVVRWARGGEFGVEFLAMRPAQLQRIQQF
                     VIKARPLS"
     gene            105436..106215
                     /locus_tag="PK224_01325"
     CDS             105436..106215
                     /locus_tag="PK224_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB14409.1"
                     /translation="MIEWVGRKAIAGYTYLAALATLFTQAVLDLVLPTRQGRGETLRV
                     LVRQILFTGVDALPVTTVIALLLGIIIVTQAGTQLPRLGAGGLVGSIIVVTVIRELGP
                     LITAFIVVGRSGTAIATELGNMSVTREVVALRLMGISISRFVIMPRMVGMVLSMLCLT
                     LYFDVVAVLGGYLIADAQLTIPFYAFVESITKALSTTDVLMTAIKGLSFGSAVAAICC
                     YHGLAVRSSFTEVPQQTTRAMINSFVLCLLIDVVVTVPLYL"
     gene            106212..106943
                     /locus_tag="PK224_01330"
     CDS             106212..106943
                     /locus_tag="PK224_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB14410.1"
                     /translation="MNEAALVLEQVRTPMAGSPHVLDVDLRIAAGEFVALVGPSRSGK
                     SLVIELAAGLVRPQAGRVVLLGHEWNDTPESEQTPVRLRVGTVLLQPGLLSNMTLFHN
                     VLLPLRYHHGAMPERSREQAVMAQLERLGVAPMRDRFPAELNQGEVRRAAIARALMLD
                     PEVLLLDDPVAGLDAEMVLALKACVEERRAHHPLTVVAALRAFSPFIEAADRLVVLRD
                     GRVEADGARDTVARTVPEMLRKYVE"
     gene            106989..107978
                     /locus_tag="PK224_01335"
     CDS             106989..107978
                     /locus_tag="PK224_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRB14411.1"
                     /translation="MHYSHRLSSGRLAQIVGTFVLIPVLILAVAGFWMSKTEHLFEPK
                     YYLMASLSKSYGLEPGAPVVVSGIRVGRVKQVDLNDRGTVDLTLQLSARYQDMVKGNS
                     ELHITKSGVVVGQTQVEIGAGTASSPVLTDGAIIRAVEPRDIGDLLNEVEPVLMAVKQ
                     TLLRVETITQDLQGGLKAGGKALEQVAVATQDLPAVVASVQRTIASVEQTATALPAMT
                     GSVQRTLAVVDRVTADMQRTTSRLPAILDSAQSTLTSVKRLSDSVEEVSHELLPVVHT
                     AETTLADVSILVRGAKQTFPFNRFAQNAGPAPRPEAALSPTAPPSLRGDHVRR"
     gene            107975..108931
                     /locus_tag="PK224_01340"
     CDS             107975..108931
                     /locus_tag="PK224_01340"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB14412.1"
                     /translation="MIVRGHGCSSGFWGVTLCLLLGCTVAPPPPPVPAPRPLSVQVES
                     LRLQGDRWMIRGDLARAGEAYEEARRLAESLDDMKGLVGALNDLGSVALGRGAGREAI
                     QLHGRAVSLAQQFGETDLLIAGLASLGAAEYQEGRTEEAGRRYQQALDLLQRAADQRT
                     EAMLRNNLGLIRQATGDVGQAEQLFRQAMALNQAAGHPAAEASNHVNLGILAEERREY
                     EVAEREFERALELDKTAERRAEIAADLLRLGRVAERRGFPDRGLAYSERAYRSYLAQG
                     NQAQAIAAITFALDCARRLGLVEEVARLEKELIRLTGSRSGR"
     gene            complement(108960..111272)
                     /locus_tag="PK224_01345"
     CDS             complement(108960..111272)
                     /locus_tag="PK224_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRB14413.1"
                     /translation="MQPKPPLPGLGDATLKSGNAENVKRISGQILAASDVDQILLDLR
                     HEILSCFDAEDLTLFVVDSEKKEIFSKIPHLDTVQEVRTPITEQSLAGFCAKYLRPVN
                     VGDAYSPVELSAIHPSLTHDASYDKNTGFKTKQVLTYPVVAENKYLMGVIQLLNKKSG
                     GRFTRKDEECVAEIAKALGTAFYALRKTSKKPPSKFDYLLTNGKVSQHDLDNALAEAK
                     KGTTDIESLLIEKYKVPKAELGKSLANFHKCPYIEYNERTIVDIELLKNLNVDYLKKN
                     HWMPLKRDRAAIEILTDDPGDLDRVQDIKRTFPGLNIRFAVSLRRDISLFLASTTGTP
                     EITNKMNENVSDILGELVNESQLEAQEDASSAGLDENDNAIVRLANQIIADAFRQGTS
                     DIHIEPYGEKRDTLVRFRVDGDCFEYMKIPPSYRRAIVSRLKIMASLDIAERRKPQDG
                     KIKFKIGENKEIELRVATIPTAGYNEDVVMRILAASEPLPVDKMGFSERNLREIKSIA
                     EKPYGIILCVGPTGSGKTTTLHSVLGYINTPDIKIWTAEDPVEITQYGLRQVQVHSKI
                     GFTFAAAMRAFLRADPDVIMVGEMRDKETADTGIEASLTGHLVLSTLHTNSAVETITR
                     LLDMGCDSFSFADAMLGVLAQRLARRICKDCKEAYQPSKEEYEELRLGYGPDYWDTLG
                     VTFDANFRLYRGKGCDTCNRTGLKGRVALHELLLGTDQMKKLIQNKAKTDEMLKAGMA
                     EGMTTLVQDGIQKVMQGHTTYKEVKAVAIK"
     gene            complement(111485..113191)
                     /locus_tag="PK224_01350"
     CDS             complement(111485..113191)
                     /locus_tag="PK224_01350"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRB14414.1"
                     /translation="MRVSETLIPTLREDPGEAETVSHRLMLRAGLIRKVAAGIYTYLP
                     LGLRVLRKIERIVREEMNRAGAQELLMPVASPAELWRETGRWDFYGKELLRFKDRHER
                     DFCLGPTHEEVITDLFRREVRSYRQMPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDRDEAGARLNYQKMYDAYNRIFTRCGLTFRPVEADTGLIGGTSSHEFMVLAETGE
                     ETIVYSEDGTYAANVERAEVLPPDTADNSAHRPLTPVSTPNRRTVEEVTAFLKIEPRQ
                     LVKTLLYSTGKETVAVLVRGDHDVNEIKVKRLLGVPEIELLKPELVPGLTGAPVGFVG
                     PVGLKQVRIMGDWAVKAMANFVVGANQADTHFVDANWERDFKVDQFADLRNARAGDCS
                     PRKDGTLKTAKGIEVGHVFMLGTKYSQAMKATFLDAQGQEQLAVMGCYGIGVSRTAAA
                     SVEQNHDAKGIKWPVPIAPFHVTLLPLSQSQPVTQLAETLYRAMQEAGIEVLWDDRDE
                     RAGVKFNDADLIGAPYHLVIGEKGLAQGQVELKLRHTGETQKVAPDQVVSLLSSFLKA
                     AS"
     gene            complement(113198..114589)
                     /gene="rseP"
                     /locus_tag="PK224_01355"
     CDS             complement(113198..114589)
                     /gene="rseP"
                     /locus_tag="PK224_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRB14415.1"
                     /translation="MGTAFAWSPDVVSLLTHWALPFLVVLGVLVAFHEMGHFLAARWV
                     GVKVLKFSLGFGPKIFGRQIGETEYLLSVVPLGGYVKLFGEDEHETLTPEDKKRAFVH
                     QSLWGKTLIVAAGPIFNFILAYLIYTAYIGLGYTLPVPSFKDIIPEVEAVLPGSPADQ
                     AGLKPGDRVIRVNEKEISTNAELLKYIAQSNGKQLTLDLTRGEQVKTVLVTPSKTTVQ
                     DNGKATTIFQLGIEERAPVITAVIPGSRAQAAGLSAGDRVVRIDGHDIFTWSQMTSLV
                     RESPNRALQFDIQRGDTTQTLSVTPMGEKATVEGKPAEVGKIGISAQNQTILQTNDPL
                     KAPWLGAQATWGWTELTVVGIYKIITGDISRKNIGGPLTIAKTAGDAAEQGTSSLVFL
                     MAMLSINLGVLNLLPIPILDGGHLLFFFIEAIRRKPLEDRQRELAQQVGLVLLVGIMI
                     FAFWNDIERLISP"
     gene            complement(114615..115775)
                     /locus_tag="PK224_01360"
     CDS             complement(114615..115775)
                     /locus_tag="PK224_01360"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRB14416.1"
                     /translation="MKNIVILGSTGSIGTNTLDIVDRFPQEFRVVGLTAGSNDEKLEA
                     QIRKFRPAFAALANEAAAARLRDRCADLPVKILAGNEGVAEVAQSQEAELVISAIVGG
                     AGLLPTLAAIRAGKQIALANKEPMVMAGALMQAEAKKHHVRIFPIDSEHSAIFQSLEG
                     HRREDVKRIILTASGGPLWGFSREQLQDVSPERALQHPNWKMGSKITIDSATLMNKGL
                     EVIEARWLFDIPETQIEVLVHRESIIHSLVEYTDRSVIAQLGLPDMRTPISYAMRYPE
                     RMPLDLPSLDLTEISTLTFFKPDHERFPCLQLGYEALRIGGTMPATMNAANEVAVEAF
                     LQNGIRFLDIPDIIRSTMESHAPRPIDALDDALEADRWARDKAESLVHALTR"
     gene            complement(115808..116656)
                     /locus_tag="PK224_01365"
     CDS             complement(115808..116656)
                     /locus_tag="PK224_01365"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRB14417.1"
                     /translation="MPPTLDRARPRSSSETVRRVLAAAVFLPIFYYLVHDLGAIAFFG
                     LAAIAGMLAAGEFYRLHLGQAPWPWWCWLGVGATGLLISSAQWPTLVTDRAVLLGTLA
                     IALCMPLLSGKSLRDSLTDGMVLVMGVLYLGLTLSYLVLTRGLPDGALLIFFVFLVTW
                     AGDTGAYVAGKSMGRHALAPVISPKKTYEGLAGGLVLACLMAVVARAWFLPAFSLVDC
                     LALGMILTITGLIGDLAESAMKRSAGFKDSGALIPGHGGMLDRLDSLLFTGPAFYYYV
                     TIVNNG"
     gene            complement(116700..117485)
                     /locus_tag="PK224_01370"
     CDS             complement(116700..117485)
                     /locus_tag="PK224_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRB14418.1"
                     /translation="MNDSRSRDIEPAPDSDLLSQLEPDLLPKHLAVIMDGNGRWAELR
                     GLPRIAGHQEGIKSVRELISLSLELGIKVLTIYAFSQENWNRPAQEITALMGLLEHYL
                     STERSSLVEKGVRFQTIGRVTALPPSALQWVRTTEQETAHLDKLILNVALSYGGRAEL
                     VDAARELARAVQSGRLSPDQIDEQAMQQALYTHHLPDPDLLIRTSGETRISNFLLWQL
                     AYTELYFTPTLWPDFRRRETLVALIEYQRRERRFGRVLSSVSS"
     gene            117678..119114
                     /gene="tldD"
                     /locus_tag="PK224_01375"
     CDS             117678..119114
                     /gene="tldD"
                     /locus_tag="PK224_01375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HRB14419.1"
                     /translation="MSNDESGALARFGVTDREIEQALGRVKVSTVDYADLYFEYCQSE
                     SVSMEEGIVKRATKSVGQGVGVRATAGEKTGFAYSDELTPKDLNIAADTARYIANSAQ
                     GTAPVPVTHRPALARNLYPHDRANIEVATRDRVTLLNAIDAEARRYDPRIKNVMAAYN
                     TEYKVMLVATSDGLMVGDIQPLSRLQVTCIAEENGNRQVGTFGGGGRIGLEYFQNDQR
                     YLQFAREAAREAILNLSAVDAPAGVMPVVLGGGWPGILLHEAIGHGLEADFNRKKTSA
                     FSNLLGKKVASEVCTIVDDGTLPFRRGSLNVDDEGTPTSRTFLIERGILRGYITDKLN
                     ARLMGIPVTGNGRRESYQSVVLPRMTNTFMLAGESDPKDIIKSVKKGLYAVSFGGGQV
                     DITNGKFVFSASEAYLIEDGQITKPVKGATLIGSGPDILKQVSMVGHDLKLDEGIGTC
                     GKEGQSVPVGVGLPTIRIDEITVGGTKA"
     gene            119117..120475
                     /locus_tag="PK224_01380"
     CDS             119117..120475
                     /locus_tag="PK224_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRB14420.1"
                     /translation="MTRVIAEQDYTNIAQDLLARAAKHGATASDVMVADGETLSVQVR
                     MGAVDRLTKAREKRLGLRVFFGQRSASASTSDFSRESLERFVGETCALAQAVVEDPVS
                     GLPEPGQFATDFPELNIHDSTKLQTDQQIDLALRAERAAFAADSRITNSEGGECDSSS
                     GRIILANSHGFVGHYANSSFSLSVSPIASDAAGMQRDYWYGVNRAFAKLESPEAIGRE
                     ATRRTVRKLGARKVSTCRVPVIFDPEVAGSLLSHLCSALSGYALYKGASFLIGQLGQQ
                     IAPDFVTIYDDGRMPGGLGTRPFDGEGLPTRKQAMVERGRLASYILDTYSGKKLGLPS
                     TGNAARSIGESPSAGPTNFYMVPGTTSPEDILASVKQGLYVTDLIGFGINMVTGDYSR
                     GASGFWIENGELAYPVEEITIAGNLKQMYANIETIGTDLVFRGRIASPTVKLAEMTLA
                     GN"
     gene            120497..121051
                     /locus_tag="PK224_01385"
     CDS             120497..121051
                     /locus_tag="PK224_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRB14421.1"
                     /translation="MRRLMASMICALLVGPAVGRAAEFQVTSPTIKDQSTIGNEHVFN
                     GFGCTGGNLSPELRWDHAPKDTKSFAVTVYDPDAPTGSGWWHWLIFNIAPSVTHLPAG
                     AGQPESAGAPQGSIQSMTDFGQPGFGGPCPPPGDKPHRYIFTVFALKVDQLPLKKEAS
                     GAMVGYYLNQNAIGKASFTGTYGR"
     gene            121088..121543
                     /locus_tag="PK224_01390"
     CDS             121088..121543
                     /locus_tag="PK224_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14422.1"
                     /translation="MSLDAELGKTMLQLITSRYEDRHWRKKIEKTLSLPQSGVGDDHQ
                     QQVFMYLKLNLKAYKSRRADPDSWILGGYATKEVIDRAKFQPHLVGEGISADDVAFLG
                     VDPGPEIDEAWWEEMLVAWFGVPEEEESSEEATADEAATPDAVKDPART"
     gene            complement(121607..122278)
                     /locus_tag="PK224_01395"
     CDS             complement(121607..122278)
                     /locus_tag="PK224_01395"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRB14423.1"
                     /translation="MSSSLTTLQELAESLHNCQRCPLAKLGRRQVVFGVGNPHASVMF
                     VGEAPGFHEDQKGEPFVGAAGQLLNDLLESAGLSRADIYIANVIKCRPPNNRDPEPQE
                     VETCKPFLLQQIAMIRPKLVCSLGNWATQTLLERKVGITRVRGQAFYLKEFVLFPLLH
                     PAAALHQGTMLQPLREDFQKLREFLDRHSQPPATQEGAASSTTETLRIDPPATIAQQM
                     DLFGS"
     gene            complement(122369..123679)
                     /locus_tag="PK224_01400"
     CDS             complement(122369..123679)
                     /locus_tag="PK224_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB14424.1"
                     /translation="MNRTVLILGGGIAGLTAALHLAADGHAVTVIEQADHLGGRLCHA
                     PPPLLLEAHTATWSLLKTLGKDTATRRLRHTPLEFLQATGARIQFLHLPLPSPLNTLL
                     GTTLFQGLSMRDRWHLLSFLERTWEQDPPLPNDLDTRAADEWLASIGQSEHARHGVWN
                     SLARLLLGAALPQVSAELFMRTLRRCFLTGARATKLIIPPHGLDSFLLTPLRAQLDRM
                     GVRSRLNTTATQLHFTQDRVAHVELADRTRLTADWYIAALPHHRLTPLLPERVVTHYA
                     YFQQISRLSESPLVIVRLHLAQPVEQTQLVLLERNRFHWMIRHADEERHEQAAVLWAV
                     AVDEPDLLPQSKDDLVQLTLKDMERAFPGGPLPRLIESDVVRLPSAILTTKPGTQQCR
                     PLSHSPFTNFLVAGAWTDTGWPASLESAILSGQRSAALVPADAS"
     gene            complement(123676..124551)
                     /gene="hpnD"
                     /locus_tag="PK224_01405"
     CDS             complement(123676..124551)
                     /gene="hpnD"
                     /locus_tag="PK224_01405"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HRB14425.1"
                     /translation="MMTPAEAQTYCTTLTKKSGSNFYYSFLFLPKARREAMYTVYAFC
                     KEVDNAVDEPPPGSHPQEELARWRRELAAAYDGTPTFPVTVSLARHVRELSIPQAYFE
                     ELIKGVEMDLTTTRYATFDQLSLYCYRVASVVGLICLHVFGTTSPRAQDYAVNLGMAF
                     QLTNILRDLGNDAECGRVYVPQEDLARFAYREEDLLHRRYKPEFTEFMRFEVGRAKEF
                     YAKAARALDSLPRAERRALTVAEIMRGVYSRILHRIEQSGYRVLGDRVTLTPSHRLAV
                     AAGVWLRSRLPSSTP"
     gene            complement(124559..125491)
                     /locus_tag="PK224_01410"
     CDS             complement(124559..125491)
                     /locus_tag="PK224_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRB14426.1"
                     /translation="MKICSLVPGATEVVAALGCQHDLIGISHECDFPPSLAQIPVMVR
                     PRIESHHLSSAEIDQQVGALLTAEAGLYELDETRFLAAGPDLIIAQALCDVCAITPSQ
                     LERVVHALLPPPRLLTLSPGRLDDILLDIETIGTAIGREEAGTQLSAVLRSRLDTVRA
                     TVGAQATRPRVACIEWLSPLYTAGHWVPDMVAAAGGIDVLAQAGTASRKMDWDTLTAS
                     EPEVIVLMPCGFTVPRTKAELASMTAHPQWQHLPAVQRGEVYLVDALSYFSRPGPRLI
                     DGVEQLAAMLHPASYGRHLPASVERLEGQTTHSH"
     gene            complement(125488..126195)
                     /locus_tag="PK224_01415"
     CDS             complement(125488..126195)
                     /locus_tag="PK224_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRB14427.1"
                     /translation="MSELRAIIFDFDGVIADTEPLHFAALRQVLAGIEISLTEAEYYT
                     DYLGFDDRGCFLAALQSHRRQASPTLLGELMEHKAHAYLAAVKQHLAIFPGVRELIHE
                     AAARYPLAIASGALRNEIELILEEAGLRKAFLHITSAEDVTRGKPAPDPFLHAMAGLN
                     SQPNQPALSPNDCLVIEDSLPGIRAARAAGMKVLAVANTHTVQDLGEADAITHSLADT
                     RLQDLQARLWGAAQGRP"
     gene            complement(126354..127097)
                     /locus_tag="PK224_01420"
     CDS             complement(126354..127097)
                     /locus_tag="PK224_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14428.1"
                     /translation="MVSSTGIPAMRSEWTNWHLLFASQPEPDLEFTEEDLDESMPPPA
                     PPMNSPKRSGKKPLLWILLLLLVGGVGYVAMDPDGAMQLVEPYLGGGAEPAQPVIQKP
                     PVKAQAPKQAPTVAPPVVADNSTPAPADVPAPSSAMSAPAIPAPAMPALQPAASAPKP
                     AAPTVRVAGPLYAEGQRVTVIADPTRPKSPIPLFVDAVGTKTSTTVLAGATLTILDGD
                     YQKRGWVYSVRAQDGRKGWVPERSLRLKR"
     gene            127725..128138
                     /locus_tag="PK224_01425"
     CDS             127725..128138
                     /locus_tag="PK224_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRB14429.1"
                     /translation="MKSYREELWFETKTRRAYVNITPEVDAVVRKSGVREGMVLVNAM
                     HITASVYINDDEAGLLRDYDEFLERLAPHEATYRHNDTGEDNGDAHIKRQLMGREVVV
                     AITDGRLDFGPWEQIFYGEFDGRRRKRVLVKVIGE"
     gene            128301..129314
                     /locus_tag="PK224_01430"
     CDS             128301..129314
                     /locus_tag="PK224_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14430.1"
                     /translation="MLSWSRPDPLTRDLIHLINARRHDHGDTDAAIDTLQAFIEQGRE
                     QDLLTVLAALDEEMAEWMFDLVAEASCAVIMDGPDDDKGAYALMAALELPLQANLGQP
                     LGLKIDPVATADLLHRHLQIPAGVVVRVEPRLLTDATLEGLTLQTLNEHLVSIADSQR
                     TQAIASRLYPPVENTAFLFFQLIGMPDPGLPDSLEEPERRALLEGLVAQCAELALAPD
                     TLEVPVYASYALFDAQNAVRLHRLADETAAVWRDLDPMVRARTIAHLHTQCGNGVYMP
                     VWTDLFDPELPEDHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVEA
                     PEN"
     gene            129437..130045
                     /locus_tag="PK224_01435"
     CDS             129437..130045
                     /locus_tag="PK224_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14431.1"
                     /translation="MAGPSRLGSIAIVTVLSCVVLGAVWGLVLYTLPQSDLAPEPTPV
                     SFPPQPATPASPAHTGVPAPPTPGPVSQPVVREPAPVIQIEAQEHPPVEPVRAPFGRE
                     LKCDLEIEALCPEEEGERRACLQRKAAQLSTPCRPMLRERLVRMKEAMQQLRIACEAD
                     RRQFCREVPLGGGALVQCLESHAQEVSDQCFQLLPKRGRLLN"
     gene            complement(130215..130946)
                     /locus_tag="PK224_01440"
     CDS             complement(130215..130946)
                     /locus_tag="PK224_01440"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRB14432.1"
                     /translation="MSQHHPASPTLLVKAPQGGTRELEVSRTPFTIGRKHDNDLCLED
                     LAVSAHHARIVQVQQVLFLEDLTSTNGTFVNEQKIDRRQLRDADSIRLGTHRLIFREA
                     HPITAETAGTGEILITDDTMVVSRPMGGERPPTEQKIGVVEILSGKSGQSQYHLTKHV
                     SLIGAQDDAVITLTGWFAPKTAAVINRRGEGYVVSQTESGKRILVNGRHIQGEHALRN
                     GDVVEVAGLTMRFLLRDPKGSLVAG"
     gene            complement(130956..131792)
                     /locus_tag="PK224_01445"
     CDS             complement(130956..131792)
                     /locus_tag="PK224_01445"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRB14433.1"
                     /translation="MHSLHVTHGATSDVGLTRAHNEDRFHADPSAGLFVVCDGMGGHR
                     AGEVASSRVIDIIPRHLAEAETDPSLPLIGAVRPEFSSATNRLASAVRLANHKVHQEA
                     ARHVEYAGMGTTVVAAWLVGPILSIAHVGDSRLYLIRGNAMQLLTADHSLVQEQVQSG
                     LLTAHDATRAPHKHVLTRAVGVQPLVEVELGELPVLNGDLFLLCSDGLTAGVSADTIL
                     RVVQESSDPQTVSDRLIALSNAAGGTDNTTVIVAMLRHRKPGMWHRMCTQLFAAPTTE
                     SS"
     gene            complement(131801..133210)
                     /locus_tag="PK224_01450"
     CDS             complement(131801..133210)
                     /locus_tag="PK224_01450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRB14434.1"
                     /translation="MTTSWGWIGPYLIVIILAIMVGPLLATLPLFTHTFLQPLRMNAA
                     QAVRLIADGICLLMIWLTAARARQDLHDNGKGQTFFRAILFPSALLLIVLVATRAYEA
                     HGVPVLGTPKQPLYNWLITGGLIGAAIWLTFAWVRHADALTRAFVGRPRRRPVEEEDA
                     QADTAGEARATADAVTPNPARAGGTVIVRNGAAVPDSLGRYQVVRELGRGAMGVVYLG
                     KDPTIQRHVAIKTMRLDDIDNEEELKEFRDRFFREAESTGRLSHPNIVTVYDAGEQEG
                     LAYIAMEYLEGTLLSCYCQKSTLLPAKQALQIVATVADALDYAHSQGVIHRDVKPPNI
                     MILKQRLVKVMDFGIAKMASGSKTQTSMILGTPRYMSPEQAMGKDVDSRSDVFSLGIV
                     LFELLTGERPFDAENMPALVTRIAKAPHAPLLKYRRDLPTRVQAILDRALQKEIPNRY
                     RHASDMAQDLRDVFQVMPR"
     gene            complement(133289..133453)
                     /locus_tag="PK224_01455"
     CDS             complement(133289..133453)
                     /locus_tag="PK224_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14435.1"
                     /translation="MNTRSVVRSSRPAGLFSLGLLDGLHVRLRRPYVREAPAGFGLKR
                     LATAFMNNPG"
     gene            complement(133534..134382)
                     /locus_tag="PK224_01460"
     CDS             complement(133534..134382)
                     /locus_tag="PK224_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRB14436.1"
                     /translation="MSRIDLHLHTTHSDGSFSTRDVMAFAKQAGVTALAITDHDIVEG
                     IAEATAIGAELGIEVVPGVEISSRLGESELHILGYFLNWTDPLLAQRLSTLRDSRHTR
                     NPKIVQRLNELGIPITYEEVRALAGTESVGRPHIARLLMEKKFVTSAKEAFDRYLANG
                     RPAFVDRELPEPAEAVRWIREAGGVPVLAHPTWVRTSADGLRTLVRDLKAAGLGGIEV
                     HYSTHTPSQTTEYLELAKQCDLLVTGGSDFHGVTKPDIEVGIGRGQLKVSEKLLDPLR
                     KAATSN"
     gene            complement(134379..135308)
                     /gene="folB"
                     /locus_tag="PK224_01465"
     CDS             complement(134379..135308)
                     /gene="folB"
                     /locus_tag="PK224_01465"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRB14437.1"
                     /translation="MAPRIIIERLECYGRCGVTEEERGKPQLIAIDVELEAAVDAAGL
                     SDRLDETIDYARVAERLVALNGSLTCRLLETLAEQLVGMLFAEFPVDRVQLWIRKLHA
                     PLAMVAGSVGVRVDRTRTAQQAGPSPSPFLTQQLGRLPKGRVLDVAAGRGRHALHLLS
                     HGMQVEAIDRDALALTALKKAAEERQLSGLTTRVLDLEEKPDHPPDLGRECYDAIVVF
                     FYLHRPLFPVLVDALKPNGILIYETFSIENYFRHQHPRRWEFCLAQNELLRLTAPLRV
                     LHYDEGEHDGGHGSGPCYTARLVAQKAGPELPR"
     gene            complement(135486..135956)
                     /locus_tag="PK224_01470"
     CDS             complement(135486..135956)
                     /locus_tag="PK224_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14438.1"
                     /translation="MFLPARLFGSKNRRTPLQLTPRRLTGQSVDEQIRRLQSAWGDVL
                     SLPMCLGALALYEWWRWLFSIPSNPLLLTIVAAVAIHATWRRRAVYKAELKLLRLGRG
                     DEPTVAQAAELLRLTAARLLQHIANIRIPSTILSSVRRALSPANGLAKRLAARL"
     gene            complement(136077..136316)
                     /locus_tag="PK224_01475"
     CDS             complement(136077..136316)
                     /locus_tag="PK224_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14439.1"
                     /translation="MADPSHVSFSDSLTLARRTAFYERRKPIAVVMILLVFLLPFFGV
                     FVRGLIGAAAGVIVSVLCYYLTPYLVLKLNGETNR"
     gene            complement(136923..137441)
                     /locus_tag="PK224_01480"
     CDS             complement(136923..137441)
                     /locus_tag="PK224_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14440.1"
                     /translation="MKMKRLVGMGVMVMALVLSDAIEAADLTGAWKGLLSSADGSQAE
                     VQIDFSPQGFPLYSYTNNKGLTRQVELSQVGQTVEYVPPGGGVQRMVVKRVEQGQGRL
                     SVGIVGSFERASQGYLDQRQEAALFEYALVPGGLKMRVTTSSTSHFGDKDLMVGGDPD
                     AVVAEGVLQKLH"
     gene            complement(137470..138381)
                     /locus_tag="PK224_01485"
     CDS             complement(137470..138381)
                     /locus_tag="PK224_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRB14441.1"
                     /translation="MATTTSSSSSKSKGLSLHIGLNAVSPAAYGGWSGDLAACEFDAK
                     DMAAIAKSRGMKSTVLLTKKGTRAKTLAAMRKAAKALRKGDFFFLTYSGHGGQVPDVT
                     GEEADKQDETWCLYDGQLIDDELYFELSRFAAGVRILVFSDSCHSGTVTRNRPPQVDP
                     AAPVRRSKMMPISVGLRTYREHQQFYDSLQKNVAKAAGKASAADPDSVLAQLAVSPRL
                     TAIAKDFKASVILISGCQDNQTSMDGEQNGAFTEQLLKVWNHGNYSGNYAKFHAAIRA
                     ELPASQTPNLFTLGAAARFVRQRPFTV"
     gene            139412..141859
                     /gene="cas3"
                     /locus_tag="PK224_01490"
     CDS             139412..141859
                     /gene="cas3"
                     /locus_tag="PK224_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HRB14442.1"
                     /translation="MSEVNQFANWFARATGNEPYPFQIRFACEPKLSELVDVPTGMGK
                     TAMAVLGWLWRRRFAGERVRKATPRRLVYCLPMRVLVEQTVENAQDWLKNLEPLGELE
                     KGKVSVHVLMGGDIDNDWDAHPEVDAILIGTQDQLLSRALNRGYAMSRYRWPMHYALL
                     NNDCLWIMDEVQLMGVGVTTTAQLQAFREKFGTFGPTQSIWMSATLKTATLGTVDFAE
                     RAETLPRLSLNSDDIKHSHVANRVNAVKLLEKAETAWRQETKDSYADQLAEEVCRHHQ
                     PGTLTVVMVNRVGRAQDMFVALKRQVRSEPSQPDLCLIHSRFRQAERKRLQQQLTESP
                     GQGRIVVATQAIEAGVDVSARTLFAELAPWSSLVQRFGRCNRYGEWEQKSSARVFWID
                     LQTSTTNKKGEQQLVPEAALPYEIDDLDWSRDQLNHLKAVGPNKVSTIAAPEKDEFGH
                     VVRRRDVVDLFDTTPDLAGNDIDVSRFIRDGENNDVQVYWRQWDGEAPSADMAAPRPE
                     ELCAVAIGRIKEFLRKDIVAFRWDGLEQHWLPVKKDNVWPGLTLLLHVCEGGYAEDLG
                     WTGKNDGAVLAVPLSPELPSNEALDEDRFTFINRFVHLTKHATDVAEEMNALKTALDD
                     PTFKVPWSELLTAARWHDVGKAHEVFQRMLTDPLLPNNPNKSGGPWAKSDHKKGRPDR
                     KHFRHELASALALLLHKESDLTAYLAASHHGKVRLSIRSLPGEQVPSDARRFARGVWE
                     GDKLPGIDLGDGITTQAVSLTLSFMEMGEGPCGASWLERMVRLRDHYGPFKLAWMETL
                     VRVADWRGTAKKGEANA"
     gene            141852..143924
                     /gene="csx17"
                     /locus_tag="PK224_01495"
     CDS             141852..143924
                     /gene="csx17"
                     /locus_tag="PK224_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csx17"
                     /protein_id="HRB14443.1"
                     /translation="MPDLQLAGCRPEPLAHYLKALGILRLVSEQADPEVRGWWVDDEF
                     RLRTKLSREKLEKFFLEDYRPTPVVAPWNGGSGFYPNDRKDAIEAIFSTRAGRFLDYQ
                     ATVKQSKEIIQLLALKDKPDVDAKPRLLRACRGRFSEKSLAWVDAAYVLTDEEAKFPP
                     LLGTGGNDGRLEFTNNFMQRLMELFDPLTGHPEVLSKTWLSSALWSEPVTGLLTDRPI
                     GQFFPGAAGGANATVGFSSRSLINPWDFVLMIEGAVLFGSAATRRLQGSGGSEVGYPF
                     VTRAAGVGYPSASGSDETPRGEMWMPIWTAPVRISEVAALFSEGKAQVGRRTAKTGVD
                     FSQAIAALGTDRGVQSFCRYGFQVRNGLAYFATPLSRWRVVSRPEANLLFEFDHWLVR
                     FRREATGNTSPARMGQVLRAIERAIMEYCQTGHPSRMANLLIALGAAEEALARSPQFR
                     GKHFLKPLPLLSPKWLDAANDSSVEFRLAASLASVGLRENMEPVRVEAAWAGWLDTDT
                     PPRIVWGHGSLTDNLIAVLFRRCMDAQREQRKGLPLAGRYTTSLNDIHEFIAGNVDER
                     RLEGLLRGLTLINWRLVRESTQATDEHESLLPGLYALLKLTHLPNPFRGNSIPYMPAI
                     LVRAAAGDASQASRQAIRRLRGCGFIPAVEAISEPANVTRRIAGAVLFPISKPQEASL
                     AEVILRTQ"
     gene            143978..144943
                     /gene="cas7u"
                     /locus_tag="PK224_01500"
     CDS             143978..144943
                     /gene="cas7u"
                     /locus_tag="PK224_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HRB14444.1"
                     /translation="MASEVNFSELQNAPRLLIEADLQPIQGTRFQPTGFPDLGAATYQ
                     LHDGTEMLLVESPQSMANRLETVCWDESAGDLVKPLAGLPYIAVKEDEKVITNSLLES
                     HRINSPYILEGRDKSFFETLKKELGAMEKRPVDLQMLASVLLKYDANALLHGVFLAKS
                     DLAGGRLRLPRSLSAFIEAKGSIVAASGGVKMDRVDPQGDTSKGFGHVPFHRNEYTAE
                     KISAYFNLDLIQVRGFRLGDKVESLLIALALFKVQKFLAEGLRLRTACDLEAVSISAK
                     KPAGFTMPSLKDIAANLPALIEAVASENKFAKPAITSVSYKGDKK"
     gene            144940..146499
                     /gene="csb2"
                     /locus_tag="PK224_01505"
     CDS             144940..146499
                     /gene="csb2"
                     /locus_tag="PK224_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HRB14445.1"
                     /translation="MIALRFRFTAGRYHATPWGRHVNEGAVEWPPSPWRILRALIATW
                     HRKFSREIPEHSFRPLIERLTDLPVFYLPKAALGHTRHFMPQKDPLGADKTKIFDAFV
                     SVYPDDGFIVAWPHITLSSEEREVLTSLASGLSYLGRAESWATAEMLDQWDGDINCRP
                     IEGGVSVEGERVRMLASMTPDSFVTWKMGYETKVVGETTIVTKAGRKKKVSQSPVPPN
                     LWSALHVETSDLQKQGWSAAPGSRWVDYERADDAFRVTYQRKPKIEQIRPTVARFALS
                     SAVLPRLTDVVFLGERMRQALMAKSRGLNGSEHALPVFSGKGADGLRLDDDHSHAFYL
                     LSDEDGDGRIDHMTVYATQGFDKHAQQAIGMLRKLWGAGGHDIHTVLIGIGQPQDYGG
                     FLMREGEAPALAQSRIWRSVTPYILTRHPKKNGKDSPAAQVAKDLLRRGFPKPERIEH
                     VDHTKITGKKVRWLEFRRFRSGGGGQIADSRGFGFEIVFAEPVRGPIALGYGCHFGLG
                     QFVAVQEAREA"
     gene            complement(146545..146736)
                     /locus_tag="PK224_01510"
     CDS             complement(146545..146736)
                     /locus_tag="PK224_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14446.1"
                     /translation="MTKLPQVSHERVVRALKREAKHISMTTDKHLVIIPHHHSIKPGT
                     LKQILEAAEIPEDRFKELL"
     gene            complement(146733..146933)
                     /locus_tag="PK224_01515"
     CDS             complement(146733..146933)
                     /locus_tag="PK224_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRB14447.1"
                     /translation="MPPIVIEPDLEAGGYVVSCPTLKGCVSQGETEEEALENIKDAIK
                     TYLESIEDLKRLKKLRTVQVSV"
     gene            147017..148717
                     /gene="cas1"
                     /locus_tag="PK224_01520"
     CDS             147017..148717
                     /gene="cas1"
                     /locus_tag="PK224_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HRB14448.1"
                     /translation="MDTGSQGPSTGGDIPLLPARMVNEFVYCPRLAYFEWVDGEFADN
                     AETTEGRFHHRRVDQAPVRKSRQTENQESGETTIHQRSVWLSSEGLGVTAKIDLVEGE
                     GQAVVPVDYKRGKRPHTTQGAWEPERVQLCVQGLLLREQGFTCERGVLYFVGSRERVE
                     VLFDEELVARTREFLDGMRTMTQATVAPPPLVDSPKCPRCSLVGLCLPDEVGWLRQPH
                     NGDVPALRKLIPTNDDALPLYIQTPGAKLGKDGECLKVKDHDDVIGEARLVETSQVVL
                     YGAVQVSTQVVQELCKRNIPLVYCSSGGWFYGMTTGLLHKHVELRRRQYAVAADRERC
                     LQLARRFVQAKIANCRTLLRRNHRAAPDTVIQDLKNDHAHAGTVESLESLLGVEGTAA
                     RRYFSEFAGMLKEAEPGARFDFEGRNRRPPRDPVNAMLSLLYSMLAREWTVTLQSVGL
                     DPYLGFYHQPRYGRPALALDMMEEFRPLIADSAVLTAINNGEIQQSDWFERMGSVTLT
                     PEGRRRLIETYERRMGQEITHPVFGYQISYRRVLEVQARLLGRYLLGEIPELPPFTTR
                     "
     gene            148721..149011
                     /gene="cas2"
                     /locus_tag="PK224_01525"
     CDS             148721..149011
                     /gene="cas2"
                     /locus_tag="PK224_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRB14449.1"
                     /translation="MRRHYLVTYDICDAKRLRRVFKTMKGFGAHLQFSVFQCDLPDID
                     LVRMKAALTEIIDQREDQVLIIDLGPTESHPVKRIESMGIKAEWEERRARVM"
     repeat_region   149224..151890
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=149224..149259
                     /rpt_unit_seq="gattccacggctcagatgccgtggccccattgaagc"
     gene            complement(151938..152138)
                     /locus_tag="PK224_01530"
     CDS             complement(151938..152138)
                     /locus_tag="PK224_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB14450.1"
                     /translation="MERNLRLDWPRLVEEAVKRRKEQKLTQKTLAVLAGVSGPTVNAF
                     EQQRTSITLESALKIFRCLGMA"
     gene            complement(152087..153292)
                     /locus_tag="PK224_01535"
     CDS             complement(152087..153292)
                     /locus_tag="PK224_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRB14451.1"
                     /translation="MANLLWGNVYYQDRFAGVLRQEPGERMSFTYDEHYLAGRHPSIA
                     HSLPLRTDTLVSQSGLPPFFDNLVAEGWLEEAQTRLLGKRRVSRFELLLAFGQDCAGA
                     VSVIDPDPEHRDAARPDDPMDLAVLSGRASLSGIQPKLALVRRDGQFRPARTDELSTH
                     IAKFPSPRHGDLTANEYLTTQALQAFLPGDEIVDLRMGHVAGFEEPTLIITRFDRTNG
                     GQRIHFEEFNQLLGNPSHAKYEGSHKSMADFIRQTPGCLPAEVYRLYLRILAGILLGN
                     TDMHLKNFAMFHTEAGLRLTPAYDAVSATLYGYNTIALSIGGSAHIPISEVKARTLIR
                     LGEEFGLSKPAIAMAAGHLEKRREAARNAIAESTTGTPELRDAIITFMEKRWNGTFAL
                     IGHALSKKP"
     gene            complement(153559..153708)
                     /locus_tag="PK224_01540"
     CDS             complement(153559..153708)
                     /locus_tag="PK224_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14452.1"
                     /translation="MKQGYRLIVVDKDGVLVSEFQLTEQALAAPEAFVSALRESVEAI
                     EEAEP"
     gene            153912..154946
                     /locus_tag="PK224_01545"
     CDS             153912..154946
                     /locus_tag="PK224_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HRB14453.1"
                     /translation="MNTTKLMAACLVLSLVGVPTISFSKARGGSGGGFSSGRSSGSSG
                     SMGSRGSRTYQDNGAKPIEQSTTARQATSPPPSVASGPASTARPTPAAQPSWIQRNPL
                     LAGIAGGLAGSWIGHMLFGATDSSARTNEAGEQVGETGQAGASGPSGMLILLMLMGAG
                     ALFFYLRSRKPAVAPDFSGITRSSAVSGSLLDDSSVSTLRTATIDSEITSADKAAFQQ
                     SLTDIQTAWGKQDLSALRRLVTPEMLEYFSTGLAELTSQYQANRVEDVVLLEAEVRES
                     WVENTVQYATVSLHWSARDYTVSLSKAPGDAGYLIEGSAEKPIETTEVWTFMRYQHGK
                     WLLSAIQQVA"
     gene            155313..155978
                     /locus_tag="PK224_01550"
     CDS             155313..155978
                     /locus_tag="PK224_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB14454.1"
                     /translation="MRIAHISPFLEKVSSESQDPQGRMIFHLTKQLTRLGHEVTVFAS
                     GDSRTAGELIPVAPLSMRSYPAPKRHLAEALAMLALEKAFATLPSFDIIHLHSGLSCL
                     PLMRRSPIPIVSTVYDALDAPEVIKVYREFKELALIATCAEQLKQVPDLNWQAVIPCL
                     DMLRWKNEPQAVEKIARAHETVYEWSTIGMGLPERVAPHLRVVTSAKANTTHERSIAA
                     SPY"
     gene            156221..156904
                     /locus_tag="PK224_01555"
     CDS             156221..156904
                     /locus_tag="PK224_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB14455.1"
                     /translation="MRILLVEDDADLAQFIRKGLKEERYAVDVASNGEQGFELGIANP
                     YDLIILDIMLPVLDGLSLCRRLRDTGVTAPVLLLTARDTVQDKVSGLDMGADDYLTKP
                     FAFAELLARARALLRRGGPQLQARLTAADLELDPATHRVCRSGNEIALTNKEYALLEF
                     LLRNKNRVLTRTAIIEHVWDISYDPMTNIVDAHIRSLRAKMDRDFSPALITTVRGAGY
                     MLEDEEAGR"
     gene            156901..158346
                     /locus_tag="PK224_01560"
     CDS             156901..158346
                     /locus_tag="PK224_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14456.1"
                     /translation="MNSLGRLIRRTALILMGGLLLGFSALIYVGGDTLLSRYVDGRLL
                     ELAETLGRIIEQRPDVIRGAGDELVVLGEKGRSQEAQHELREAAHSVRILSIDGQLLW
                     KGSDVVPRPPVSASLLEQVKQGQTVFDMVQLREGSPVRRVSIPVPRSGQVRYILQAEE
                     SLHFSKETLKGLAILLALGSGLVIVVAWAGSAWIARTVLTPIGLLSRRAETMSEADLG
                     ERLSLDSPFQEFHRLTHTFNAMMDRFQKSCESQRRFVDYAAHEMQTPLTVLQGNLEVT
                     LQKARSTDEYREALIGNLEQVEKLIALARSLLTLTKVTGDRPPLQLAPLELEPLLQEL
                     IIELKLLADDRRISLALDVVPVPTISADAQWLKQALINLLDNSLKYTPPGGSVTVRLR
                     RSDESLEIAVRDTGPGIESEHVPFLFDRFYRADKARARESGGTGLGLAIVKGIVEAHG
                     GTVSVETQPGEGAEFTIRLPLGRAPEGHPVT"
     gene            complement(158352..158522)
                     /locus_tag="PK224_01565"
     CDS             complement(158352..158522)
                     /locus_tag="PK224_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14457.1"
                     /translation="MHTLISAAEEFVNGVPYVQELDKERNALLDAITQAQTLMSAEHT
                     AVTRPAKRRQKR"
BASE COUNT        32200 a        42785 c        49661 g        34163 t
ORIGIN      
        1 gtgacggtgc tggcggggtc gcttccggcg ttaccctggg ccgtaggatt gctggtggtg
       61 acgattgccg tgatggggtt cgggaacggg gtgatttttc aaatcgtctc cgagtggttt
      121 ccaaaggaca tcggtctggc ctccggcctg gtgggcgcgg ccggcgggat cggcgggttt
      181 ctggtgccca tcgggttcgg gttgctgcgg gaaagtacgg gcacgttcct gttcgggttc
      241 gtgggattgg cggtcgtgag tgccgccgcg gcgatgacgg tggctccggc cctgcggtgg
      301 cgtccgcagg ccgtcgcgca ggccccgagc cccggtggtt tttcccgttg acaggggtgg
      361 tttgaaaaga gttagctggg ccaccaaagc aggctggcct gcaccatcga gggattatgg
      421 cgacaccgtt taattcgatc gaagacgcga tcaaggatat caagaagggg aagttcatca
      481 tcctcgtcga tgacgaagat cgtgaaaacg agggcgatct tgtgatggcg gccggcaagg
      541 tcacgccgca cgccgtcaac ttcatggcca aacatgcgcg tggcctcatc tgtctggcgc
      601 tgactccgga gcgggtggag gagttgcagc tggcgccgca ggcggcggag aacacggcca
      661 ccttcggcac cgcgttcacg gtctccatcg acgctcgcaa agggatcacc accggtatct
      721 cggcggcgga tcgtgccacg accattcatg tcgcgatcga ctcgcgttcc acaccgggcg
      781 acttggcgcg ccccggtcac attttccccc tgaagtcaca agtcggcggg gtgcttcggc
      841 gcgcagggca gacggaaggg tcggttgacc tagcacgatt ggccggactc tacccggccg
      901 gcgtcatctg cgagatcatg aacgaagacg gcacgatggc tcgtgtgccg gaactgacca
      961 agtttgccag gcggcacaag ttgaaaatgg tcacgatcaa ggcgttgatc gagtaccgca
     1021 tgcatcgtga gacgttcgtg cggcgagccg ccagtgcgtt gttgcccacc accttcggcg
     1081 attttgaagc gattgcgttc gagaatgaca tcgacggcgc gacccacatt gcgctggtga
     1141 aggggcaggt ggatgccgag acgccgatgc tggtccgggt ccattccggt tgtctgacgg
     1201 cggatgtgct gggatcgttg cggtgcgact gtcgtgagca actccatcgg gcgatggaga
     1261 tcattcagaa ggacggtcgg ggcgtgttgc tctatctcaa tcaagagggg cgcgggatcg
     1321 gcctgctgaa taagattcgt gcctacggtc tgcaggattc tggcagcgat accgtcgaag
     1381 cgaatctgca gctcggtttc aaggccgatc tgcgcgacta tggcgtcggc gcacagatcc
     1441 tcgtgcagct cgggctgcac cgcatccggt tgattaccaa caatccgcgc aagattgtcg
     1501 gcatcgaagg ctacggcttg aaggtggtcg aacgggtgcc gatcgagatc gccccccgtg
     1561 ccgacaacga aaaatatctc cgcaccaaaa aagccaagct cggccacctc ttgaaaaagg
     1621 tctagcgtcg ctccagccgg tctcaatcct gagtcatcct cgtgtgggaa agatgcgtgc
     1681 gccgacggcc tgaaacgtgt agccatttga gggcgtttcc aggtatgatc cccctctttc
     1741 ttcggcgcac acagtccatg agacctctga ctcgttatct catttcatga agcttcgcaa
     1801 aggttcccaa gacgcgacgg ggctgacgtt cggcatcgtg gcgagcaaat ttaataagtt
     1861 tgtcaccagc cggttattga cggagtgcgt gaaggcgctg acgaaggccg gcgtgacgga
     1921 cgaggccatc gaggtggtgc gagtaccggg cgcgtttgaa attcccctcg tggctcgcca
     1981 gcttgcccgg tctgggcgat tcgatgcggt gatctgtctc ggcgcagtga tccgtggtga
     2041 tacgccgcat ttcgagtaca tcagcaccga agtcagtcga gggattctgc aagcctcgat
     2101 ggattcgaat atcccggtca tcttcggcgt cctcaccacc gataccgtgg ctcaggccat
     2161 tgagcgtgcc gatccggaga agctgaatcg cggcgccgat gcggcgaaaa cggcgatcga
     2221 aatggtcaat gtgatgcgac tgctcaaaaa ggaaggaagc ggggaggcgc cgcagccggc
     2281 caccccgtca cgacgagccg ggcgatcggc agcgaggccg aaacgctgac cggcgttgca
     2341 ctatggcctc tcgacatcaa gcccgtgaac gggcggtaca gattctcttt caatacgacg
     2401 ttcatggcca ggcgggcccc tggctggacg atttctggat tcagtatccc ttggccgagg
     2461 agttgcgggt attcgcggag cgattggtcg ccggcgtccg ggccaacaag aaggaactgg
     2521 atgcgctgat cgggacctat gcgaccaatt ggaaggtcag ccggatgccg atcatcgacc
     2581 gcaatattct ccgcatgggt tgttttgaat tgctgcaggt gcccgaggtg ccggccaagg
     2641 tgacgctgaa cgaagcgatc gagctggcca agagtttcgg cgacgaggag gcttccaagt
     2701 tcgtgaacgg cattctcgac aaggtgctcg ggtcggatgc acggttggaa cggaagcgcg
     2761 cgtttcccga attgccggtg gtgggcagcg acagcagcgt aaagcgtgaa acgtgaaacg
     2821 tttttcgagg gacatcccct ctggattgcc taagcgaacg acgcttcacg agcgacgatt
     2881 aacgaggaag agatgattga tcgatacacc cgtccgcgga tgagtgccat ctgggacctt
     2941 caacacaagt atgaaatctg gctggaagtg gagttgcagg cctgcgctgc gtttgagcgt
     3001 ggcgggcaga ttccgcgcgg gaccagcgcc cggattcgca agaaagccaa gatcgatgtg
     3061 gcgcgcattg ccgaaatcga aaaggttaca aagcacgatg tgatcgcctt cctcgaatca
     3121 ctgatggatt ccgtcggacc tgagcaccgg ttcctgcata tggggctcac ctcatcggac
     3181 atcgtcgata cgtcgctggc cgtgcagatg accgaagcgc tcgatctgat tctcgaggat
     3241 ctcaacgagt tgattgctgt gctgaagaaa cgggcactcg agcatcgcca tacggtgatg
     3301 gtaggccggt cgcacggcat tcatggcgag ccggtgtcgt tcgggttcaa gttggcgatt
     3361 tggtatgagg aagcctgccg gcaccggacg cgcctcgaag cggcgcgcaa ggatatcgcc
     3421 gtaggaaagc tctcgggagc catgggcacc tttgcgcatc aaggccctga aattgaagaa
     3481 tatgtctgcg ccaagatggg gctcgcgtcg gatccggtgt ccaaccagat cgtgcagcga
     3541 gaccggcatg cggcctatgc ctccgcgctt gcgctactgg ccgcgaccat tgaaaaaatc
     3601 gccaccgaaa ttcgtcacct gcagcgcacg gaggtgctgg aggcggaaga atatttctcc
     3661 gccggacaga agggctcatc ggcgatgccc cacaagcgca atccgattgc gtcggaaaat
     3721 ctgtgcggcc tggcgcgcct ggtacgcggc aatagcctgg ccgcgctgga gaacgtggcc
     3781 ctgtggcatg agcgtgatat cagccactcc tcggtggagc gggtgatcat gccggacagc
     3841 accattctgg tcgattacat gttggcacgc gtgaccgacc tggtgaagaa tctgattgtg
     3901 tatcccgagc ggatgcagcg aaacctcgac ctgaccggtg gcttggtcta ttcgcaacga
     3961 ctgctgctgg ccttgatcga taagggcgcc cagcgcaaag agtcgtacga agcggtccag
     4021 cgcaacgcga tgatggcctg gaaaggtgga gtcgggtttc aggaactggt cgctcgcgat
     4081 ccgttcatca cgacccactt gaagccgcac gaaatcaccg cctgtttcga tccggcctat
     4141 taccttcgac atttggatca agtctttcgc cgggtgttcg gagcgagcgg cgcttcgtcg
     4201 gcaaaacgac ggaggaaacg ggcatgagcc tgcggtcgac agcacgagtc gggctcttgg
     4261 ccggtttgct ggtgctggcc gcggtttggc cggtttccgc agctgatcgc gagaagcttc
     4321 tgacagagcc gggggtatac ggcacatttg cgaccttcca gatggaccat gattggtggg
     4381 acctcccggg cgagtcgcgc gtcatttcgg tggcggaggt taagggatta gtcgagcagt
     4441 ggtccggcaa gattttggtc gagtcctatc tgctccgggg gttatccgac catgccgatc
     4501 tcatgtttcg tgtgcatgcc cggacgttgt cggacacaca gcagtttctc tcggcattca
     4561 tgggcactcg attaggacgc catctcacct caggcggact tcttcatggc gtgagcaaga
     4621 agccgacgta cgttgccggc ttccctgaat ccatgaagac agagttacag gtgaacggcg
     4681 aatccggttc caggccgtac gcaattgtga tccctatcaa gaaggacgcg gaatggtggg
     4741 gactggacca ggaagcacgg accgccctga tgcaagagca tacgcaggcg gcgcttccct
     4801 atctgaagac ggtgaaacga aaactgtatc attcaacggg tctcgacgac gtcgatttca
     4861 tcacctattt tgaaacagag aagttggagg actttcacaa tctggtgcgg gcgctgcagc
     4921 aggtgaagga attccgccac aatcgacgct tcggacatcc gaccctgctg ggcacgatga
     4981 gtccgctgga tgagatcctg gaaaagttcg cacagtagtc gaaggagaga gggatgaccg
     5041 tcgaaccagc aggcacgatg atctatgagg gcaaggccaa gaagattttc acgactgatc
     5101 ggcccgacca ggtgttgcag tatttcaagg atgacgccac ggccttcaat gcgcagaagc
     5161 gcgggactat cgtcgataaa ggggtggtca acaataaggt gtcggagcgg ctgttccggc
     5221 ttctcgaaca gcacgggatt tcgacgcact tcatcgagcg cgtcagcgac cgtgagatgc
     5281 tcaccaagcg agtgacgatt gtgccggtcg aagtggtggt ccggaacgtc gttgcgggga
     5341 gtcttgccaa gcggctgggc ctcaaagaag gcgcggccat cgacccggcg atcatcgagt
     5401 tttactataa ggacgatgcg ttgggggatc cgctggtgaa cgacgatcac ctgcgcctat
     5461 tgaatgtggc gactccggct gtgttacggg agatgcgcga gctgggccac aagatcaatg
     5521 ccgtgttgct gccgtttttc aaagaacgcc ggatgatgct cgtcgatttc aagttggagt
     5581 tcggcgtctt tcacaatcga ctcatcctcg ccgacgaaat ttcccccgat acctgccggt
     5641 tctgggacca gacgaccaaa gagtcgatgg acaaggaccg gttccggaag gatttgggca
     5701 aaatcgaaga ggcgtatcag gaagtgttga agcgggtgtg cgaatagggg cggcatgaac
     5761 cggtttctcc agttgttttt gaactatggt ctggtggcgg cgattctggt gtgggcggcc
     5821 acggtggcga tgatggccta tcacctcaag gaatccccct ggcgctgggc gtttgtcctg
     5881 atgtcgctgg cagggctcgg gaccatcggc gtgatctttt ggatcaggaa gtacgtgcag
     5941 agggtctgca aggcacagca ggaagtggga aaagcacaat gaaagccaag attcatgtga
     6001 cgctgaagca gggaattctc gatccgcagg ggaaggcgat cgaacatgcg ctggactccc
     6061 tcgggtttaa gaatgcggga aacgtgcggg tcggcaagta catggaagtc gacgtgaacg
     6121 aagcggaccg cgccaaggcg gatgcccagg tgaagcagat gtgtgaaaaa ttgctggcga
     6181 atacggtgat cgaggattat cggtacgaac tcggatagga gcagatggct tatagcctat
     6241 agctgatggc agaagccgag acctcgttcc tgtctcgtat catacgccat ttgccatagg
     6301 ccataagctc tttagaatta tgaaaatcgg cgtggtggta tttcccggca gtaattgcga
     6361 tcacgactgc cagtatatct tcaaggatgt gctcggccag tatgtggaga tgatctggca
     6421 taaggaaact ctgctcgcgg ggctcgatgc ggtcgtgctg ccgggcgggt tttcctacgg
     6481 ggattatttg cggaccggcg ccatcgcgcg gttttctccg gtgatgggag ccgtgaaggc
     6541 ctttgcgaat aagggtggcc tggtcatcgg catctgcaac gggtttcaga ttctgctgga
     6601 ggctggtctc ctgccaggtg tgatgctgcg caatacctcg ctcaatttta tctgcaaaga
     6661 cgtctacgtg aaggtggaaa atgcggcgac acggtttacg aatcaatgcg agtccggcca
     6721 ggtgctgaag attcccatcg cgcatgccga cgggaactat tacaccgacc cggtcacgct
     6781 cggcggcatc aaagccaatg cacaagtgat ctttcgctac tgcacggcag acggcaaggt
     6841 gacgcccgat gcgaacccga acgggtcgct ggataacatc gccggaatca tgaatgcgga
     6901 tggaaatgtg ctgggcatga tgccgcatcc cgaacgcagt gcagaagcga tattgggcaa
     6961 tgaagatggg cggctgatct ttcagtcgat gttgagctcg ttcggcaagc ctgaattgca
     7021 atccaagttg atcggagtat gacggccggt tcgcgtgagc agcggtgccg gaactgtcgg
     7081 taacccccta cgattgagaa tgtgtcattc atgaacgcac tggtcattac caaagccatc
     7141 cttgagcagc ataagctgag cgatgacgaa tataagaaga tcctggagat cttggggcga
     7201 gaacccaact ggaccgaact gggcatgttc tcggccatgt ggagcgaaca ttgttcctac
     7261 aagagctccc gcatccactt gaagaagttg ccgacgacgg ggccgcgcgt ggttcagggg
     7321 cccggcgaaa atgccggcgc ggtggatatc ggggatggtc tgtgcgcggt gttcaagatg
     7381 gagtctcaca accatccttc tttcatcgag ccctaccagg gtgcggcgac gggggtgggt
     7441 ggaattctac gcgatatttt tacgatgggc gctcggccga ttgcgttgtt gaactcgctg
     7501 cgattcggcg gattggacaa tgcgaatacc cgccatctgc tcaagggtgt tgtgtcgggt
     7561 attgccggtt acggcaactg catgggtgta cccactgtcg gcggggagat tgtcttcaac
     7621 gacatctatg ccctgaatcc tttggtgaat gtgttctgtc tgggaattgc caagaaggat
     7681 aagatttttt tgggtaaggc ggccggcgtc gggaacccgg tgatctattt cggctcgaag
     7741 accgggcgtg acgggattca tggcgcgacg atggcctcgg actcgttcga tgaacaagcg
     7801 gctcagaagc ggccgacggt gcaggtcggt gacccgttca ctgagaagct gttgctcgaa
     7861 gcctgtttgg aactgatggc cggtgattgc ctggtcggca ttcaagacat gggtgctgcc
     7921 ggtttgacga gttcggcctg cgaaatggcc tcgcgagaag gcacgggtgt agagttggag
     7981 ttggctgatg tgccgcgccg tgaaccgggc atgacgccct acgagatcat gctctcggaa
     8041 tcccaggaac ggatgttgat ggtggtgaag gccggtcgtg aagacgaggt gatcaagatc
     8101 tgccgtaagt gggatctgga tgtggccgtg atcggccggg tgaccgatac gggcaaggtc
     8161 gtcctcaaag aaaacggcca gatggtggcg gagattccgg ccaaggctct ggccgacgac
     8221 ggtccgcgat atgaccggcc cagtgcgccg cctgcctatc aagaaatgtt gcaggcgctg
     8281 aatcacgatg ccttgccgga cgtcaaagac gttaatgccg cattgctcgc actcctggat
     8341 tcaccgacca ttgccagtaa gcgatgggtc tatgagcagt atgaccacat ggtgagaaca
     8401 aatacgatgg tgcgtccggg gtcggatgcg gcggtggtgc gggtgaaggg gacgaataag
     8461 gcgctagcgt tgtccgtcga ttgcaacggg cgctattgtc tcttgaatcc ttatgagggc
     8521 gccaagattg ccattgcgga atgcgcgcga aacctggcct gctcgggagc tcagccgatc
     8581 ggcgtgactg actgcctgaa tttcggcaat ccgcagcgcc ccgaggtcat gtggcaattc
     8641 gtacttgcga ttgaagggct caaggatgct tgcgaggcgt tgaatgtgcc catcgtgagc
     8701 ggcaatgtga gtttgtacaa tgagacgaat gacctgtcga tttaccccac gccgatgatc
     8761 gggatggtcg gattgatcga agaggccgat cgtgccgtga cgcagtggtt caaggaaaac
     8821 ggcgatacga tcattctcct gggccagacc cgtgaagatc tcggtggaac ggaatatctg
     8881 aaggtcttgc accatcgaga gcagggttcg ccgcctttgc tgagtctgga aactgaaaaa
     8941 gccgtgcagg cttgcacgat tcgactcatc cgcgatggat tggtgcagtc ggcccatgac
     9001 tgttccgatg gtggattggc ggtggcgctg acggagtgtt gcatctcggg ttcagctcaa
     9061 cagctggggg ctgtggtaca attgtctctt gatggtcttc gtcgagatgc gctgctcttt
     9121 ggggaaagcc aatcgcgaat tctgctctcc gtgaaagcgg agctggccga gcgggtctta
     9181 aatgcggcct gggatgccgg tgttcatgcc gctcggatcg gtacagtcgg aggtgctcgg
     9241 ttggtgattc aagtagacgg tgatcagcgg gcggctggct gcaaaatcga tctcgaattg
     9301 acggcactgt atgaccattg gggattggca atccctcgtg cagtaggtca ggactaggat
     9361 acggacgtca tggccaacga actcccgctg atttctcccg ataagttcca cgacgaatgt
     9421 gctgtgttcg gtatctacgg tcataaagag gccgcgaacc tcgcctacct cggtctctat
     9481 gcgctgcaac atcgcggaca ggaagcgtcc ggtattgtct ccaacgacgg cgaacagttt
     9541 tatgtcgaaa agggtcaggg ccttgttgca gatatttttt cgcaacaggc cttggcacgt
     9601 ctgcccggca cgatggcgat cgggcacaac cgctattcca ctgcgggcgg ggcaggcctc
     9661 aagaatgtgc agcccttgag cgtcaatttt gccttcggta acctcgccgt ggcgcacaat
     9721 gggaatttga tcaatgccac catgcttcgc agtgaactgg aagcctatgg cgctattttt
     9781 caatcgacgt ccgacactga agtcattatc catctcatcg cccattcgcg ggcggatacg
     9841 ctgctggacc gggtcatcga ttcgctcacc caggttcgcg gcgcattttc agtggttctg
     9901 atgaccgatc agggcattgt cgccgcgcgc gatccccatg gatttcgccc cctgtgcctg
     9961 ggacggtttc gtgacgcctg gatagtggcc tccgaaagtt gcgcgttcga tctgttggat
    10021 gccgaatatg taagggagat cgaaccggga gagttggtgg tactggatca tcagggcgtc
    10081 accagttata agccattcgc tcaaaccaag ccggccatgt gtgtgtttga atatgtctac
    10141 tttgcccgtc ctgatagccg catttttgga ggcaaagccg tctattccat ccggaaggca
    10201 ttcggccggc aattggcgca agaatcgcgg gttgatgcgg acatcgtgat tcccgtgccg
    10261 gattccgggg tgccggcggc gttgggttat tcggaagggt cggggtttcc ttttgaaacc
    10321 ggactgatcc ggaatcatta tgtgggtcgc accttcatcg agccggaaca gtcaattcgt
    10381 cacttcgggg tgaaggtgaa gctcaatgcc gttcctgaag tcctcgaagg gaaacgcgtg
    10441 gtcgtagtag acgattcgtt ggttcgggga acgacgagcc gaaaaatcgt caagatgctg
    10501 cgtcatgccg gagccaaaga agtgcatatg cgcatcagtt ctccgccgat cgtgtcgccc
    10561 tgtttttatg gcatcgatac accgaccaag aaggaattga tcgcatccag ccataccacc
    10621 gaagagattc gcaaatacat caccgcggat agcctcgcct acttgagcct ggacggcatg
    10681 gtgaaggcgg ctcccggtac tccggggcag tactgcgatg cctgctttac cgaacaatac
    10741 cctatctcct ttacccgggc agaagaactc caactagggc tctttgaagc tccgcgctga
    10801 catcgttgtc ctcgccggtt cataggatcg tctggacttc ctcctgcgcc ttgctagact
    10861 aattggcatg tcgctggttc ggaagtatcg agtgatcgtt gccactggtg ttgtggtcgt
    10921 tggggtccgc atcgcgtccc gcctgacgag tctcaggcga ttgctcggct ggttagtatg
    10981 cgagatttca gccgatccgg atgagcacgc gatggaagat gtggcctatt atgtggatcg
    11041 gtggctcgcg ctgtttccat acaatccgaa ggggaattgt tttcctcgag cgttagcgct
    11101 attttttttc gcccgccgtg ccggcctccc cgttcaattc aaatgcggag tgatgaagct
    11161 caatcagcag ctggagggac acgcatggct ggtgctggag ggtcatgagt ttcttgagcc
    11221 gtcctcctac tggcaatcgt ttaccgtgac ggttaccttc cctcaatccc tggtttcctc
    11281 aagtactcaa tcatctcgca tctcctgaga cctcctctgc acattaccgt tctcttgggg
    11341 tcaactggac ctggcgggct tgggcgaatc gagtctttcc tgtgcgtctg cgtgaatcaa
    11401 gctgacaagt ttggctttga aacccactag cggccccatc cgcacgcttt ttttatccgt
    11461 ttttcataaa aacaaaatcc ggatccccca aagggaggtg gagcccaatc tcactgggga
    11521 tagagaaacg agcgtgatga cgatagtttc gcccttgtct tgcagtgatg tgcacggctt
    11581 gcttgtagag ggacaaagac ttcggggaaa aatcggtttt agaacgaatg gtggtatcgg
    11641 agcagtcaca tgtccccagc aatctgaacc aggagcatct atatgcctgc cactgtgcat
    11701 ctgtggagtg agcctcagct cgaattgtcc gaaatgtttc ccaggcagga tgaacaggtc
    11761 cacagcacgg ttctcgatgg tgaaagtgtg ctgttgaatc tgaactccgg acgctattac
    11821 acgctcaatg tcgtcggatc aaccatttgg aatttgtgca gaggggagca gtcgttgcgg
    11881 cagattcact cagcaatctg tgccaggttt gatgtgtctg aacagcaggc gcaggacgat
    11941 atgttggcgt tgattgtgct tctggagcag gaaggattac ttcacacaga aaggaggtga
    12001 gtgagaatgg agcaacagat aaagaccgaa tacgtcaagc cggcgctcgt gaagcatgag
    12061 ttgctgcgcg acgtaacggc cattcagttg tcccgcgtgc tggatcgaac acccgtataa
    12121 catgccaggt gctctagtga gagccatgga gctggccggt taactagacc ggccagctcg
    12181 ccccacacct ctgcggtagt gacgacgggc agaagtctga aaaggagaag cggtaatgtt
    12241 catggcgtcg acaatgaagg aacttctact ctccgtccat ggcatagaga tgaaattcac
    12301 caccaattcg tccgagttgg cacattccgt ttgggcgtta ctccgtcact ttcaccgcga
    12361 caccgttgcg gctcagccca tgcggatgga gttcgacgcg atcgagcgac gtgaggatgt
    12421 tccggcccag atttccagtt cagcgaaatt gttgtatagc ggttctggcc tcgcgatcga
    12481 ccctgatcgg aagacgacat ggttgtgcga catttattca gacaacggcc tattgattgc
    12541 cgattttcat ggagagggcc ttgtcgtgat tgatggggac tcacaatctg cccggggcta
    12601 tctggtgaga ccggaggcca tggcgccgga tatccgggcg agttttgtcc atttcgccat
    12661 gacggaactt ctgaagcggc gaggattgta tacgtttcac gccacctcac tggagcacaa
    12721 ggggcagggt gtgctgattc ccggtttcag tgggcggggg aaaaccacct cgttcctatc
    12781 cctgctgcga gcagggtatc gttacctttc cgatgatcac ccattttttc ggatcagcgg
    12841 cacgcgtgtg gagatcctcc cgtatccgct caagatcaat gttactgatc acacagtatc
    12901 gttctttcca gaactgcgag aggccccgcc atctgtgctt catgccggtg tccccaagag
    12961 ctatttccat gctgaggatg tatatccggg gccattaggt cagccttgcg agcccagcgt
    13021 gatcctcttt cccgaggtgg tcaattcctc gcacagttgc ctggagccct tgtccaagaa
    13081 ggtagcattg gaaatgattc ttccgcattc cttgctggtg tatgacaccg aagtggcgcg
    13141 ccgggagttt caagcgcttg tgggattggt cgagcaggca gattgttacc gactccactt
    13201 cggacgcgat gtcatggagt tgccatggct ggtaacgcct cttctggaga agcggcaagc
    13261 aaggagagag aactgacatg ggacagtttc caaggtccac cgtcgcgcaa tggagctcct
    13321 ctcgtcttgg aggtatttcg cgtaagggga cgacaccggc gccaggattg tccctcatgc
    13381 gtacggaggg acgattgctt gcgctctgtg cgcgaacgac ggtgagtgag tttgttcgag
    13441 tcgaagtggc agatttggcc tgcgatggaa ttgattggga gctggtctgg cagctgtcca
    13501 gagcccatgg cgtggcgccg ctagtgtatc gcaatctggt cacaatttgt cccgctgccg
    13561 tgccttcttc cattcacgaa gcctttcgtc ggcacaatca ggcgaacgct cttttaaata
    13621 gcttgttggc taaagaactc gtgaccttac tcgatgcgtt ggcggccaag ggggtgacgg
    13681 cgattccctt taaaggcgtc acgcttgcac agacggcgta tggcgaccta ggtttacggg
    13741 aatgtgcgga tattgatttg attgttgagc agggagcgat tccccaagct cggaaagtct
    13801 tatggtctca ggggtatcaa ctgaccagcc aggacatggg aggcggggag gagtcagggg
    13861 agccgtatca ctttttccag aggcgaaacg gcattgttgc ggtcgatctt cagtggatga
    13921 tggctcgccg gcatttcgga tttcggcttg atcggagcgc gttttgggga aggctgaagc
    13981 ctgttcactt accaacaaaa tcggtgatgg gcctctgccc ggaggatctg ttgattcttc
    14041 tttgcgtgca tgggtcaaaa catgcctggg agcagttgaa gtgggtctgc gatgtggcgg
    14101 aactcgtgcg tcggcggccg gcgttagatt ggagtcgtgt gttgtttcag gcaggcgagt
    14161 ggagatgtcg acggttggtc ctgctcggtc tggcgatggc tcacagcctg ttcgatattg
    14221 ccgttccact gacgatactt caggagatcg agacagatgc agacatcccc gtcctcgttc
    14281 gaaagatgcc taagcagctg ctgaagaatc ctcgtcatgg gattgatgaa gattgtgcgg
    14341 aggctctgta catgacgctc aaagattcct ggcttgagcg gtggaaactt ggggttgcgc
    14401 tctgtcgcgc ggaagcagag gtgctcaccc gttcgttgcc ctggttccgg gttcaacgac
    14461 gacttcgtat gctggccact tgtttgaagc cgtttcatcg gattatcgcc aaatggattc
    14521 tttctgtccg gatgcgtgaa gcagtcgtgc ggtggttgaa gagctccggc tgatcgtggg
    14581 ttttccggca tgaaacgttt ctccttcgtc aatagataaa tggcttgcct ccctaagtgg
    14641 ttgctcccga aaaaaaacgc atgctaggat gcctgcatca ccagcagtga ttggtgactg
    14701 ctgagaacca gccgagggag cattggcctc catcattggc ttggttccag gaaggaggct
    14761 ctgatgaagc gacttctcgt agcggttgcg attgtattgg tgcatctgtg ggctgcccac
    14821 cttgtagtcg ccgccgggtt ttttaaagtc ggcgagccgg cgccgacctt ctcgttgacg
    14881 tctgtgacag gcgatgctgt ttcgcttgag cagttaaagg ggaaagtggt tgtcctcggg
    14941 ttgtttcata tttgtgaccc ctgtatgacc caagggacca acctgcaaaa ggtccatgag
    15001 gcgatgaccg ggaaaaatgt ggccgtcgtc ggggtcaatt cgtcggggga ttccaaacgg
    15061 ggcgtgggcg aattcttgag tgcctttccc gtgaaggtga cgtaccccta tttactcgat
    15121 ccgaataaga ccaccgacaa actgtatggc ggtgggaagt tcattcccaa tgtgtatgtg
    15181 atcgatcagc gtggtgtgat caggtggcag cgggtgggca atatggaatt ggctggcgct
    15241 gaggtcatca ttcaggaagt ggaaaaactg ttggcagctt cgactccagc cggcgcagga
    15301 accatgtagt ggggaaagag gatcatccat ggacgaatgg gaagagggaa agcagaagtt
    15361 tctggaagtt gtgcagagta ttgatcagac cgttcaggtc gtgattccga cgaagccaac
    15421 aaacagcatg ttccttattt ccctcacgaa ggggccgaat cggaagttca ttaccctctc
    15481 ggaagacgat attgtcgatc ttcccagcga agccggcatc tgtgcgaagg ttacaaagac
    15541 tctccaggat gcggtggcag cgttgtaatc ccatccacgt gaggcgatat ctgtgaaaca
    15601 gctcctttcc ggtatttggc aatggtcctg gttttcagaa gacaagcagc tcgattttaa
    15661 cgggctgttt ctcaacgtgg gggaacataa gattctgatt gatcctccgc cgatgacggc
    15721 cgaggcccac acatttgttc gacgccaggg tgcccttgat tacatcattg tcaccaaccg
    15781 tgatcatgtt cgtgaggcgg catcgtacca ggccgatttt cgttgccaac taaaggtgcc
    15841 tgaggccgat gtcgcgcaga tggatgtgaa gccgtccaag acattcaagg atggcgagtt
    15901 gttgcccggc ggtatttggg tcattcacct gaatgatcaa aaatctccgg gtgagtcggc
    15961 gttgtacatt cagcagggga agggcgtgtt gatcgtcggc gatgcgttaa ttggcaaacc
    16021 ggcgggaggc ttgagcctcc tggctccgga aaaatatggc gatatcacga aggcgagaga
    16081 cgggctccgg cgattgttga aatataactt cgactcgatt cttgtaggcg atggcgctag
    16141 catcctattc ggagctaaac aggctgttga gcaggcactt cagacgtagg gaacaatggc
    16201 ccttcgaaac ggactctcag aggaactcaa ccgtcagggc aacgagcatt ttgcgcgcgg
    16261 gcactatacg gatgcctacg cctgttatgc aaaggccctg gaatgtgatc gcttgaccgg
    16321 tgatcatcgc gctttgagcg cgaccttggg caatctcggc aatatttgcg cggttagtgg
    16381 tcgccgtgag tcggctcaga gctactatca ggaagtgctg gagttgcaga aggtcttggg
    16441 tgacgagaag ggcattggga cgaccctggc caatctgggg aatctccgcg ccgacgccgg
    16501 ggagtgggat cgtgcgagag cctattacct tgaggcgctc gatatcatga cgcgggtaca
    16561 cgacgagctg ggaaaggcag tgctgttttc cgatctcggc ctggtggcgc gtgaaacagg
    16621 ccagttcgaa gaggcgctac ggtactatga gcagtctctg gtgttcatgc gccgtctcaa
    16681 caatcaagga ggcgtcgccg atgcctggcg gatgatgggg cgaacctttg ccatccaaaa
    16741 gcgctacgat gacgccattg cttgttgtca taccagtcaa tcaatcgcgg agcggagccg
    16801 agatgaactg cgtacaggcg gtgcccgtta tgttctcgcc cagtgttatg aggatttggg
    16861 tcagctgcag atggccgttc agttactgga gcaggtggtg cgaatggacc gtaagtacga
    16921 tctccctaaa ttagctgaga atgtgactcg cctcgaacgg cttcgcgccc gcctaggcgc
    16981 cgaagatccg actccccagc ctcgggagtc acgcgcatga atgccgtcgc gcttccatca
    17041 tgggaacagg ctcgagcacg gctggtgagc acggttccgc aattttatga gctggagccc
    17101 ggtggagcct tggcgcttga tctgggggat gatgggtggc tgttggaggt gcggtcggac
    17161 gggcaactcc tctgccagca cggcatcgcc atggacgatg tgaagagtct gatgtgcgat
    17221 gggacaaccg aagacctggg taccgatgaa gtggcgaaac aggccaagta ttttctgggc
    17281 cctgcggtgt ctaagaagcg tcctgcgctt ctgaaggcag gcttcgcgga aaagaccgat
    17341 atgaatgacg aatatgtcgc cattacgttc cacaagacag tagactttca gcggtttgat
    17401 gaggtccttg ctgccgtgcg ctggtgccag aatctattca agattgagcc atagcacgag
    17461 ataattcagc cgggttacct cgtgatgcgc cctcccatca ccagtattga aacctttcat
    17521 cgcctcctgg ctgcctgccg attgccgcgt gtgattgtca ccgcgccgga gttgagtctc
    17581 ttaaccgcaa tgggatcggg gagctggtct atccagaagc ttgctcaggc tcttgatgtg
    17641 agcgagcgag gggtggacat tctttgtcgg aatcttgcga tgtgcggatt gttgaggaag
    17701 cgcgggaagg cctaccgtaa caccccattt tccgcaacgg tgttgaatgc gaagcatccc
    17761 aaataccgag gagcctacgt tgagttgttg cagagtcact gggtggattg gtcgcggctc
    17821 acgcgggttg ttcgaaccgg gagtccgctg gaacgcgatc agccggactt gctggcgtat
    17881 cggcgtcggt ttacctgagc gatgcatcac cgctccttgg atgtggcgaa agagattgct
    17941 gcgacacaca gggcaaggaa gcggctgtcc tatctgccgc ccaactttat ggatgaggcg
    18001 ctagccgggt cagtatgata tagtgtggta tttcaatgtg ctccatatct attccgcgga
    18061 agaaaaccag gcgctgtttc ggcgattgta ttctgcatta tcccccggcg gacgactgat
    18121 cattcaggat gtatttcttc acgaccgaga aggcctctat cccgaagaag ccagcctgtt
    18181 cgccgtgagt atgctgttgg ttacacctgc gggaaatacc tattcattca gtgaaacagc
    18241 cgagtggctt cgagcggcgg ggtttgtgcg gatcaggccg cttaggatga agaaggggac
    18301 tgaagattgg gatggggggg tgttggaggc gattcgtccg gccggaagga aagcgcgcac
    18361 cggcccccga agatcatgag gagaaaatta agcaggctgc ttccgagtgt cagtgccagg
    18421 atcggagggc ggggggaagc ctggcttgct tcctggacaa ttgtagagag atcgagtgcc
    18481 agggcaatcg tgctgtagat gacggctgcc atcacgaccc agtgttgacg gagtaggaga
    18541 attcccgcca ccagtagtgt cggaaccccg aacagaaacc ctaacaaaat accgtcccgg
    18601 atcgtggcca gcgagttggc tgatcctgcc tcaaaaagaa ggccttctag cacgaccaga
    18661 gcaacgagca gagtgatgac cggacgaaat gccttcatgg aggcactata actgccgcga
    18721 ttgcccatcg caagttccct ggcccctggt tctaatgcca ggcgatttgt ctgggggctc
    18781 gtcaccaggg atccccctgt ccccgcaagt ccaagctcac ctgaggatgc gatcatgtac
    18841 ccaacctttg aggggccagc ccggcccgct gtgtgattta cggcctgtgg gtattcgagc
    18901 gagaatccgc ctctcacagg gaggcacacg tggccctaca tatattttca gagagcattg
    18961 ctgggtggct tcttggaatg cgatcaagca gagggtcgag aaggttcggt cgtcaaccaa
    19021 aggagcgacg gttggcgtta gctttgtggg cgacgagtgt gcttgtgctc atttccggcc
    19081 tttcctcagc tgccccctcc cacgtatctc ccggcagcat aaccgcttct ggatttagcg
    19141 ggaagggcgt ggcccaagtg cctgccgatc attcagaagt gttagcagcc gggtttgggt
    19201 ttcaggcggc cgggtcgtca atgattacgg tacggactta cgatgcgccg acaggcgcca
    19261 tcctgtcgga ggattcgttc gatgtaaacg tgaaggaaga gggcgtcgcc gaacatgatg
    19321 ggaataaggg gcgcatcttt gccggtggca ttggaactga ctctaaaggg aagtcagtct
    19381 ttatgttgag agtgtacgat gcagaaacgg ggcggtttct ttgggaaggg cagctgaact
    19441 tgctcaaatt gggcgaagga gggatgacga gagccagcgc gacgatcatc ccgacccgca
    19501 catcggcgct acaagtatcg gtttccagca ccattccact tcagaccctc ttctcggttc
    19561 gggccgtaaa tcctttcacg ggcggacttg tctggcagga tcagttcgcc ccaggggttc
    19621 gaaagcgggc ccgggtcgaa ggggtgttat tcggtgggct gttcgtcagg ccggtcggcg
    19681 accccatcgg acatattttt gatttggttg tgcggaccta cgatcgcacg tccggaacgc
    19741 tgttatggga ggattcgttc gagcaactgg atcgcattga ggagtctccg accgagccgg
    19801 agcttcgtgc ccatccccag gccattccat cttggaatct tagcgctgcg ccgaggcatc
    19861 acgcgtacca gacgaagctg cgatagcctt aggattttgg ctgagccagt gtggagccca
    19921 gactgtccgc ccattgctgc tggaagtgat cgtatgacag cagcagccta tcctgcatgg
    19981 cggcagcaat tgtctgacgg gccttcaggt ggaccagcac ctcgtgaacc ttgtgcatgc
    20041 cgaaccgttc aatgagatag tgggtggcgg cattggcctc cctataagcg agactgaact
    20101 tctccgcagg cagactctcc caactaccct caagcgccgt caaaggaacc aggctgtgct
    20161 caccgggcgt gacgctggtc acctcttgcc agggatcacc ggccagctgc attgccaagc
    20221 cttcattcag ccaggttgga atcattccac cggttgcccc caattcctcg tgaatcaggg
    20281 catgcacaaa ttcgtgacga agcactcgag tcagccaagc ccgatccgtt gcagcgcctt
    20341 gggttgggat ttgaatgcgc ccaaggacgg gatcgaacag gccatccgcc caaccagggc
    20401 ttcccgtggc gccttgaaac tgattctttg tgtgcaggac gactatgatc ggctttgacg
    20461 ggaaaaagtt gaatttctga ccgatttccc gataggcctc ttcaagaata tccagcacgg
    20521 tcgtccacgt gtccgggtcg tcacctccgt cgtacttgac ggtgaagtgg acgctgctgt
    20581 gcgtcgagaa tttatcctcg accttctcgg cgcgatgtac tttcgtcgtg accgccttca
    20641 gataagactg aagaccgggg tctttcttaa ttcgttcctt ggcctgcgcg aggtgtttgc
    20701 cggcttccgg gaggtgatcc ttctcctgta gcaggtctgc caaggcaaca tgggggaacg
    20761 gctcatccgg agacagtgcc acgactttcc ggaggaaatc ttccgtgagt gcggggtccc
    20821 gcaaccccca gtaggcatgg gcgagattga ggtgggcaat cggattttgg gggtcgagcg
    20881 cgaccgcttt cttaaatgcc ttcacggaaa cttcggtccc cccgcttctt tcctggatga
    20941 ttccgaggtt attccacaag atggcaacat gccgcttggt ggcggattcc gtcaggatcg
    21001 acgacggcag ttcctccagt ttcgtctctg ctaaggcaat attgtgcttc tcgagttctt
    21061 cgtgaatggc tccgagcagc tcgggatgtc tcggaactgg aatgctgacg ggatcgatag
    21121 tcctcgaccg tttgatctcc ggggcggtac gttcctgcga tggggccaca ggagcgttcg
    21181 acgcaggggt ggtttcctcg acaatgacct gcggtgatgg agccggtgtg tcgtggaaat
    21241 agcgaggctt cagccaggtt tgatagaaaa taaagatgcc gatcaacacg gctagaggcc
    21301 cgagaatatg tttgatattg cgtcgataca taggtgtcct tccgtgaatc ctacgcgaat
    21361 tcactgtacc accgagccgg gagcggacaa agggaagttg aaaaagcagc cggggtctgt
    21421 gcgactggct accattcggc agaagtcccc tctttgagcc gtcaaggtgg ctgtggtacg
    21481 atcctgctcc aggatgccac gtcctgaatc cgccgactct cacgatcctt tccaagcctt
    21541 cctgcatcgt gtaaccagac cgattgaatt tgcttgtcgc gatgcttacg cccatctctc
    21601 ggcggtcagg aatcttgacc gttttgtgtc gcaacaggtg attggcactc ttggcgagcg
    21661 agtctatccg cgagcgctgg agacggaact gatcgcacta cgcaacctgt ttgtcgactt
    21721 tcacactcgg ctcacacccc tcgaacagca ggatcggctc acgaaagcgc tggcgctgct
    21781 cagccgccta caaggggacg cgcgagcggt gagtcagccg gtggggtctc cgaaggagaa
    21841 gcaagtacaa cctttaccgg ttcggtccag tcctgccaga ccgctgtggg agctatcgat
    21901 tcaatatgca aaaggagttg gtcccaaacg aaccctcttg ctggagcgtc tgggcgcgca
    21961 cactgtcgag caggccttgt ggacgttacc atggcgctat gaagatcgtt cggtcatcac
    22021 gccggtggcc gaacttgtgc cgggggctat acggagcgtt tgcggcgtga tcactcgggc
    22081 agaggcaacg cgtgcgcgcg ttcgccggct gtcgattctc gatgtggcgg ttcaggacgc
    22141 aacggggact gtgcacgcgg tgttttttaa tcaaccatac ttggaagatg tgttgaagga
    22201 aggcctgcgg gtcatgatgt ccgggcgggt ggcggccggt cggggtgggt ggaccgatgt
    22261 gcggttggaa gcgacgcaat ttgaagtctt gagcggcggc gaggatgagt tactccatgt
    22321 ggggcgcatc gttccgatct accatgagac gaaggggtgg acgtcacgtc agatgcgagt
    22381 tttgatgcag gggttgctgg cggagtatgg ggctgacata gaggaggtac tgcctctgtc
    22441 tgtccgtgcg cgccatcggc tgccgccgat cggcgaggcg attcaacacg tgcattttcc
    22501 cttgccgaag acggatctgg ccgccctgga tcagggggtg acgcctgctc accgacgatt
    22561 ggcgtttgaa gaactgtttc tgctacaggc ggccatggtg cttcgccaac gggagatgaa
    22621 ggaagagctc aaatcatttc ggtttaatcc tcacgtggcg caactgaagc aactggcgaa
    22681 gatccttccc tttacgctga cctccgcgca ggaacgtgtg tttcgtgaga tccaagccga
    22741 catggtgacc tcgcggccga tgaatcgcct ggtgcagggt gatgtcgggt ccgggaagac
    22801 cgtcgtggcg ctgcatgccc ttgtgatggc ttgtggatcg ggctgccaga cggcgttgat
    22861 ggtccctacc gaaattctgg ccgaacagca ctatctgaat cttgccccct tgctgagggc
    22921 agtggggttg aaggcagtgc ttctgaccag cggcgggaag gcaaaagacc ggaacgagac
    22981 gctgaagcag ttggagtccg gcgttgcgca gatcgcgatc ggcacccatg cattgattca
    23041 aaagaaggtg cggttcgcga accttggcct ggtgattgtt gatgaacagc ataagttcgg
    23101 cgtcctgcaa aggaagacgt tgctggagaa agggtatcga ccggacgtgc tggtgatgac
    23161 ggcgacgccg ataccgagaa ccctggccat gacggtctat ggggatttag atgtatcggt
    23221 gatcgacatg ctgccgccgg gccgcaaacc ggtgcgaaca ttgctctata ccgacgggca
    23281 acgtcacaag acctggcagc tggtgggaga tgagttgaag gccgggcgcc aggcgtatat
    23341 cgtctatccg ctggtcgagg aatctgagaa ggtcgatctc aaggcggcga tccagggagc
    23401 ggaacacctg cagcgcgagg tctttcctca ggcgcgggtt ggattgctgc acggccggct
    23461 tccgacggct gaaaaagagc gcaccatggc ggccttcaag gccgggacga ttcaggttct
    23521 cgtggccaca accgtgattg aggtcggggt ggacgtaccg aacgcgacag tgatggtcat
    23581 cgagcatgcc gaacgattcg ggttggcgca gttacatcaa ttgcgagggc gtgtcggtcg
    23641 tggggcacat caatcgctct gtattttgat ggccacatac cttgcgcgag aggccaggac
    23701 gcgggtcagc cgtgacggcc ggccggagca gaatgcctcc aatgcgcaac aacggttggc
    23761 cgcgctcgtg aactcgaacg atggatttgt cattgccgaa gaagatctcc ggatcagagg
    23821 ccccggtgaa tttctggggc tgcgtcagtg gggggtgccg gagtttcgag ctgcgaatct
    23881 ggtgcgggac gcgcaattgt tggagcaggc caggcaggaa gccgtgacgt tgttggaaga
    23941 ggatccgggc ctgatgttac cccagcacca ggcgttcaag gccgcgatgt ttcgtcgctg
    24001 gcggggcagg ctggccttgg gagatgtgag ctaagcagct gggacactgc cggttgggtg
    24061 gagggtgatc gatgggaatg ttgcagcgct tgagtcacga tttgcgggcc ggctgggtga
    24121 cattgcggca cggcacggcc aaggccgcca cgcgcgcgct ggaagagggc gaactactgc
    24181 gctaccgctt ggaattgcga aaggtggatc agcagcttga cgatctccac ggggacatcg
    24241 gcgaacggac gattgcgttg cacgatcgtg gtgaatcggc agaccgtatc ctgtccgatt
    24301 ctgaaatcac ccgcctgatg caacaggtgc ggacgctgca ggatgaacat accaagttgc
    24361 tgctggaaat gaacgacatc acggtcgaag gacaatgagc ctcagccgat gaccaaccag
    24421 acaacggctt ctcctcgaat tttcgccggt atcgatatcg ggacgctcac ttgtcgtttg
    24481 ctcatagcgg aactgacctc tgccggaggg ttgagagagc tccgatctga ccgacgcatt
    24541 ctccggctcg gtcaaggggt ggatcgggag cgcgtgctgc gtcccgatgc catggcacga
    24601 gtggtggcca cgttgaaaga atggcgtcag gtcatcgacg gctatcatgt tgacgcggca
    24661 acggccgtgg cgacgagtgc ggtgcgcgat gccgggaatc gtgatgagtt cctaggcctg
    24721 gtccaacggg aagccgggtt ccaggttgag atcatttccg gcgaggagga agcccgccga
    24781 accatgcttg gtatccgttc cggcctgccc tctggtgtga cggatctgct ggcgttggat
    24841 atcgggggag ggagcaccga gtttattctg gaccggcccg gccaggcgac gctggcgaga
    24901 tccatcgata tcggtgtcgt gcggttgagc gaacggatac tgcacagtga tccgccgacc
    24961 ccagacgagg tgcaggaagc gcgcgactgg gtgcgccggg aaaccactgc cgcaatagca
    25021 gcaatgccac ggtcgccagg agtcatgttc gtcggcaccg caggaaccat cactgcgctg
    25081 gccgctatgg ttcagcagtt gcctgcttat gagccggcaa gaattcacaa ctacacgcta
    25141 acgctaaata tggtgaagca gttggagttg cagctactca gttgttcgaa ggcgcaacgg
    25201 gaaggcctgc ccgggcttga acgtggacgg gaagacgtca ttgctgccgg tgcggtgatc
    25261 ctaggtacgg taatggacac gttgggaatg acgaaagtgc tcgtgagtga cctggggctg
    25321 cgggagggtg tgctgataca tcttgccggt ggtcgataag tgctattttt ttcagtcatt
    25381 cgatttattt ttgagcgcgt gctgatcaat gacaatcctg gcttctgttc agttgaaggc
    25441 tcagttaaag cgtaaggttt ccctctgtcg gatcgcgatc acacttgagt ggcccctagt
    25501 gctgaaattg aggaatcgcc agaacgttac ctctgcgaat accagcggag gccgctcttc
    25561 tgctcggtga aggtcggttg ggggatgccg cccggctttt ccaggagtag caccttgaag
    25621 tcgatgaggc cgaaccaggc ggggtcggcg atgtcgtggt agtggcagcc ttggagcttg
    25681 gggaggatgt cgcccagggt tttggtgagc tcactttccg tgtaggcccg cacagaaaag
    25741 ttctgcccca gtccctgaca gaaccgttga atcgtatagg ccgcgccggt gcagaggact
    25801 ttcgtgtccg gcttgatctg caaaaactgc gggagtgaca ggtaccgctc ctgaaagcca
    25861 agccaacgta ccgcttgtcg caggtggttg tactgcacct cgtattccgt gtggtggggg
    25921 agcttcacgg cggcgggcca accttcttcg acgccaaact ccgtgagaaa tgcccgaccg
    25981 cccggcttga gtacccgcca gacttcggcc acgaaccgca tcggtcccag attgaagatc
    26041 acttcttcgg gcaattcctt ctcaagcggc aacttcagcc gcctgatcca atccagcgct
    26101 tcttgatgtt gtgcggtctc gcctgtgcct tccgtgagct ccttgcggct gagcttcacc
    26161 ggcgtcatgt cggccatatt ttcgttgtcg atgatgaggt cgacggagtt gtctctaaag
    26221 ggcaggcatt cgccgttggc atgggtgccg gtgccggtcc agccgccagc cttcgcgcgt
    26281 ttggtctgga gctgcaggaa cgggcgcgtg acatccaacg aaatgtattg aatgccttgt
    26341 ttctcgaagg gcagtagttc ctgtcctagc tcgcgggcga cataacccaa tccaccaccg
    26401 atctcgacaa tgacttttgg cttcggtttg aaccagccga gccggcgtag ttggcgcatg
    26461 agcaggcggc cataggtcgt gccgcccagg gcttcgcagg gttcccggaa gaggtgggag
    26521 acggtggttt cgatcagatc gaagtggcca tcctccaggt cactttcctt caactcgtgg
    26581 acatggaaat cttcgaggtg ttcctcacct tcgaactcct gctgccccga ccagccttct
    26641 cggatttgct gcaggagcag gtcccatttg gccttgtggc gatggccccc gggcggctcc
    26701 gtgccgaagt agcacagcga ataattgggc gtggaccacc gtgccaggtg ccaggtcccg
    26761 ggctggccgt ccgggccgca gatcttatta ccgtacttct ccacgagcgc gcccaccgta
    26821 atgtggccgt tcatggaggt gagcatttca atcccgacgg cattgagccg gacgagcggg
    26881 aagtcgtcgt cgagcggcgc tagccaccat tcgtcgaagg cgtgctggta ggctacgagg
    26941 tcgggaatac gccaggcaag caggttgagg gtgtcgccgc tcaatgtgag gggctcttgg
    27001 atggctgcgg cagctttcat ctggttaggt cctaggcacg aaaagccgca gcctacacga
    27061 gcgcggcgct gacgtgcaag aacctcgatg aggaggagtg gctacgtctg cgagggaagc
    27121 agcggcgact ccgtcacgat aatcatgccg cgcataatgg ggtgaatgcg gcattgatag
    27181 gggtaacggc caggcggcag gcttgggatg gtgtaggctc ctcccggttc gacggcccct
    27241 gagtcgaagg cgcaacggcc ggtttcttca aggcatgcat cgtgggtcac ggtatggtgg
    27301 gtgggcgttg gattttccca acggatggcg ccgccggcgg ttacagtcac ggctgcaggc
    27361 acataatagg gtgatccgct gtccatcagg acttgcgtcg ccggtggcgt cgcagaggtc
    27421 ggccaggcta tcaaggtgct tgtcgccaat acgaagaaga acagtcgcca gctcatagcg
    27481 tcgttattcc ggtgaatcaa tatacatcta ttatacatag agacaggttc ccgacggaag
    27541 gattcagtcg gcctatgggg aggcattcgc ttgggctgga ggacggaaca cgccgggtcg
    27601 tgctggatgc gcccgagcgg cgccgacggc acgaatgtga atgcttgcag gggcgcgaca
    27661 gcgttgcagt ctgacacagt cagccgtggg tgcaggatgg ttgcaagaac ccgcttgttc
    27721 atgctagatg gaggccgctt taccaccgac gtgccatgct gaagacctga gaggaggacg
    27781 tggaatggcg aagaaaatcg tgaaaaagaa aagcgttgct gccaaagcct cgccgccgaa
    27841 aaaagtcgcg gcgaaaaagg cgacgaagct gaagaaagcc gtggcagcta agaagcctgt
    27901 gaaggccaag aaggcggtag cggtaaaggg gaaacctgcc gcgaagaaag tcgtcaagcc
    27961 cgcaagaaaa gtggcccaaa aaccggctgc agcggccaaa cgtccttcca aggcgaagcc
    28021 cgtctccaac gcggtgaagc cggcgccgaa agccgtcaag cctgcggcaa agcccaaacc
    28081 caagcctgcg ccccccaagg tcaaccctcc ggtcgaacag tcggccgtga ttcctgagtc
    28141 gaaacctgcg aagcctccag tgccggaggt cagcaaggcg gcgaaggtgg agcccgagcc
    28201 tcaaccgctg gagctcgacg acgaagagga aatggcggaa gaccttgagg atgtgtcgga
    28261 agaaatcgaa gaagagatcg aagaagaact cggactcgat ggtgaagacg acgacgtgga
    28321 ctatctggaa aagtcggaag atctcctcga cgacgcagac gagtatcgca acaattagcc
    28381 cacgttggtg tcgtggctta ccgggttgtg ctcggttgat gcctctccgt gtcacatgct
    28441 ccctagccag tgaaggctcc actgaacagg cctgatggtg tcttccgtcc cgacaggtct
    28501 gagactgtct cggggagagt cctcgagcca gttgctgtgg tgctcggctt ctggccatca
    28561 tcctgatcct ataaatttca tgagccattt tttgtttatc gccggcgccc ttcgtgaacg
    28621 aacttcccag gagagtgccg agctgcagtt tggcgccggg atttgggggc tctgcacggc
    28681 cttgattcgc gacaatctcc agaagttttt ggcgcctgac tcccatggat tggtgtatgt
    28741 gctgaaggaa ggcattcgag cggagtttgc gattacttca ccggtccagc cgtttcaagc
    28801 gctggatgaa ttgctcaagg acgaggtgcg aacagaagcc cgatatggat tcgttcggat
    28861 cacgccgctt cggcgttggc aggttcatgc gccggactgt caggccctgc ttcagcgggt
    28921 gttggggatc gaggagcagg cagaattaac tcggcggcta agtctcggca tgcatcgtct
    28981 cacggacgcg gagtttcggg cgatcgtcga agggttgggg gtggaggcgt agcccgcgag
    29041 gtccactgac cgcgggactc ttattcgaat gtgcctccgg tcatgagacg gtaggcctcc
    29101 tcgactgtgg cgacctccgt gacttcaacg ttgagctccc aggcgagtcg gttgaggttc
    29161 cagcgcggat cgtcccgctg gccttggggg atcaggatcg tgcgataacc ctcgcgggct
    29221 gccgcacgaa tcttgtcggg gatgccgccc acgggtccga tggtgccatc cttggcaatc
    29281 gtaccggtta aggcaatccc tcgccggata tgatctcctt tcaatagcgc cgcaaatccg
    29341 accgtcatca ctgccccggc gctcgggccg tctgtcccgg ttgcggcgta tgcgatgccc
    29401 atggcactga ccgttcccgc atgatcgatc tgtggcgtgt gggctagcgc atattgaaag
    29461 gcaaggacca tggaactgag gttcgtctgt ccgggccgga tgttgccgcc cagccattgc
    29521 agctccatgg gcgaggggct tggccggtga tcccaacgca acaggagcac ttcgaacacg
    29581 ccgaggttat tcgattggat cgccgcgagc atcgggactt cgaccaccgc ttctgcgccg
    29641 gcgcgcggga tagttgcgag tagcacaacg gtgcaggcaa agaccgtgca taggaagcgt
    29701 cggcctccgc tggtatgaac cacgggtgta atcgcggaca tatgagtcga agtgtagcaa
    29761 aagcattggg ggagtaacgt gttcgcaccg ttatgtgttt ctcggttggt tcggagaggt
    29821 tttgtggaat catcgaaccg acccgctgga tcatgtcgat ttcccatctg ggccgcaata
    29881 gcggtgttgt ggggaatggt gctgctcccc gttgcactgc gcgcggaatg tgccgcggag
    29941 acgggtatga agaacgccgc ggctgtgttc accctgcatt taccgaatac ctgtactgag
    30001 gctgagcggg aggcgcgtgc cgtatccgcg caagaattgc tgcgggcgct ggcggcggga
    30061 aagggtctgg acctgtcggg ggttgtgatt cagggcgacc tggtgctcga tgagctgccg
    30121 gctcagaagg tgagtatggt gggcgacctg gcacccgagg accgtcgagt gcttgagggg
    30181 ctgaatgatg aggaagtgca tgtcattcgc gggccattcg tgatcaagca gtcgagggtg
    30241 aaaggacgga tcgtcaaccg gctcaagagc gggtttctgt tgatcaccgg accagtggtg
    30301 ttggcgcaca cggatttcgc cggattcgtc gacctctcgc gcaccgtgtt tctcggcctg
    30361 gtcgatggat cgaatgcgac cttccatcag gagagttatt ttgttcagga tcgatttacg
    30421 cagggagcca tgttttcgga tacgcatttc ggcccgcacg ctcgatttca ccgctcggtg
    30481 tttgccggcc cggcgatttt tcgcggggcg acctttcagg ggttaactga gtttctggaa
    30541 gtggtgtttg agcaggatgc aaatttttcg cgagcggcat ttcacctcgg caccggattc
    30601 tccggtgctc attgccgtgc gaagtgcgac ttctcttcgt ctcagttcga tcgggaagcc
    30661 ttttttctgt tcgccatctt cgaccgtccg gctacgtttg cctccgcgcg attcggatcg
    30721 caggccgact tttccgatgc cgcatttaaa caggccgatg atctcgcaca ggcgaccttc
    30781 gcgcgcacac cgcagctgac cagaaccgct cgtgtcagca caacggtgcc gggtcctacg
    30841 gcttctacct cggctccatt ttcccaggcc gtcacgatca tcctatttgt catggcgctg
    30901 gggctgttgg tctatatcat cagggccaag tagccctggc tgaacccaca acatatagtt
    30961 ttagtcttgc ccggtccccc cataaattgg tataagattg aaactttggc caagggagcc
    31021 tctcgtacct gatggaccaa cctgaactgc cctaccaagt caagatcgag aatttcgaag
    31081 gtcctctcga tctcctcttg cacctcatca agaaaaacga aatcaacatc tacgatattc
    31141 cgattgcgct gatcgcgcag caatacttgg actatctgtc ggtgatgaag gaattgaacc
    31201 tggctgtggc cggtgaattc ctcgtgatgg cggcgaccct gctgcatatc aagtcacgga
    31261 tgttgttgcc ggtcgacgag gtggcggtgg acgaggagga cggccccgat ccgcgcgaag
    31321 aactcgtccg gcgactgctc gaatataagc agttcaaaga ggcggcttcg cagctggact
    31381 ataaagaacg tctatggcgg gatgtgtttt cgcgcgagcc cggtccgccg gtcgaggtca
    31441 agaaagacga gagtctgctc gacgaaattt ccctctttga tctggttgat gcgctgcagg
    31501 gggtcttgtc ccgccatcca ggcaagcgac ttgttgaaat cattccggac aacctcacgg
    31561 tccgcacccg tatgagcgcc attatcgagg cgctggaact gcatgaatcc atggccttta
    31621 cggcgctgtt cgaggagtcg agtcatcggc ttgtggtgat cgtgacgttc ctggccctgc
    31681 tcgaattgat tcgaatcagg gtcgtgcgcg tgtttcagag tgaaactttc ggggcgattc
    31741 ttgtctcgcg tgccttctca gcggttgcgg aaggggagct agtggaggag tctgaatgga
    31801 agaaactatg agtcccggcg tggaggaggc aatcgaagaa tcggtcgagg tgcgggaccc
    31861 ggtcgacgag ccgactgcat cgctcgatgc tgtcgaggtg gagcccgagg ttggccaaga
    31921 cgttatcact gaaatgtcgg ccccggcggc cgatcctgcg ctttccgata tgcgagccct
    31981 gaagggcatc cttgaggcct tgctctttgt gacggccgag cctattccgg tcacgcggtt
    32041 cttagcattg ctcggagcgg ttacgaagca ggatgtcgat caggcgctcg cgagtcttgc
    32101 tcaggactat gagcaggaag gccgcgggtt gcagttggct gaagtggccg gcggttaccg
    32161 gatcgtcacc aaggccgaat tcgccccgtg gctcaaacgg ttggagaagg taaagtctcc
    32221 ctcgaaactc tcgcgctcag ccttggaatc cctggcgatc attgcctaca agcagccgat
    32281 cgttcgcgcg gaggtcgagc agattcgagg ggtcgaaaca tcaggggtca ttcggacgct
    32341 gctggagcga aaactcgtgc gcatcgttgg ccgcaaggaa gagccgggcc gcccgatcat
    32401 gtatgggacg accaagtttt tcctcgaaca ttttgggttg cgcgatttat ctcagctgcc
    32461 gccgcttcgt gagttcaaag agttgggcga atctgagcag gcgatgttgc cgattgatga
    32521 ggtcgtggca gttgatggag agcccgtgac gcaatctaca gaggcggtcg tcgaagggct
    32581 gtccgaaggc atggatgagc ctgtggctct tcttgagccg gtagatgaag cggggcagac
    32641 ttcagagtca ctcgttctgg agaatgaggc cggaggggac cttgtagtcg ccgatatcgc
    32701 ggaagagtcc tgactgctgg tcgcaatgtc tgctcaaaga ctccgataga ttcgccccgc
    32761 attgccgccg gcgctttttc agtcgttatg cataaaaggc cttcgagaga ctcctgtgaa
    32821 gggggttttc ccgaaggcct tttgtgtctg acgatcagca gcagttcgcg gctgcgagca
    32881 cctagtggca accgattgga acaaaacccg cgccgaagat ctgtgacagg gtgacgtatc
    32941 gggcattgtc atagaatgag taactcgggg tgtaccagtt tcgggccgtc ggattgctgc
    33001 cgtacgacgg atcggtgccg aagaagtgac cgccacggct gttctgattc agatgaatcc
    33061 attccatata catgctgcaa acttcgccct gcaccgaccc gccgctggtg ttgcccgcat
    33121 accagtcgcg tcctgactcg ggtatctggg agagattgcg caaggcgccg atgggggtgt
    33181 cgtatgaaaa atctttcgct ttcggggtcg gctgataggt cgaaccgttg ggaggggcga
    33241 gtggcagatc tggtagcgag ggcacaacgg agagcgcttt gaggaccttg gcctgacaat
    33301 cgggtcgctc tttgttggtg taaaggatcg ttccctctgg gcctgggcat tcccacattt
    33361 ctacagggga tgactcgacc gcgtgcacgc tgggagtaag gagcagactc gcggcgaggg
    33421 ttgcgagcac aagtcgtgtt ggccacatgg tacacctccg atttgttcat cttaccgggc
    33481 acgcgcgttg ttatccaacg tcgagaatgc agagcctatg acattcgaaa gaaatgcgtc
    33541 catccagctt tggggggatg gatgcctacc ctgcccctcg tcctagcgcg cggcttcgta
    33601 cgctctggtc ggcgcggtga ctccgttgcc gatgtatcag cggaagagcc tccagagcct
    33661 cccaggccac gcgggtcttg ccttgactcc cttcttttcg ctttgttaga ctgcccgatc
    33721 taagttattg aaacatccgc caatcttcac caatctcgta ccacgaggaa caccatgatc
    33781 aaggcttggc tgctcgacga aatgtttcgg tttgaccggc ggtatctcac ggcggaggga
    33841 agtcagagtg agtggggcgc cggggactcc gttgctgagg aggaagacct gcctcctgca
    33901 ccgactgggg tggccgctca agctggaaat ggccgggtga tgattacctg ggacgctgtg
    33961 ccggaggcca tgtattacaa cctgtatttc atgaccacca agggcgtgca gattaaattc
    34021 tccgaactca ctcgccccat cgccagcgcg gaagatttca aaaccgtcat cggcgtgacc
    34081 aaggaaaagg ggacatgtat tgaaggtgcg cagtcgccct tcgtgcatga cgatttggca
    34141 aacggaacgt gctaccacta tgtcgtgacc gtggtgacgc agaaggggga gagccccgaa
    34201 tctcaggaag tcatggcgat cccggctccc tacctcattg ccaagatcat cgggcaggag
    34261 ggcgtggagg acggcgagtt cagttctccg actggtattg cggtagacaa ggacggcaat
    34321 atctacgtgg ccgataccga taatcactcc atccagaaat tcgacaaaga cggaaaattt
    34381 ctcggtcgct ggggcggcga agccggcagc gcagaaggcg gcttctatta tccgcgcgga
    34441 ctgaccacga attcggacgg gcaggtgtac gtcgccgata ccggaaacaa tcgcgtgcag
    34501 cgcctcgaca ccgacggcaa tcccatgaag gcctggggta agttcgggtt tgcctggcgt
    34561 ggggctgaca tgaataagtt cgacgctccc tggggtgtga ccaccgatca ggacggcaat
    34621 atttacgtga ccgataccaa caacgcccgc attcaaaagt tcaagggcga tggaaccccg
    34681 ttgttgaagt ggggtcggga tggaagtttc gacggggcct ttttcttccc gcgcggtgtg
    34741 gccgtggact tcgtcgggaa tacgtatgtg gccgatgaag ggaataatcg cattcagaag
    34801 ttcgataccc gcggaagctt tttgaccaag tggggccgtg aaggaagcgg tcccggccag
    34861 ttcaaagccc cctggggcgt gacctgcgat gccttgggca atgtgtacgt cgtcgaccag
    34921 ggcaaccacc gtattcaaaa gttcgacgga aacgggacct tcctctgtgc ttggggaaat
    34981 cgagggaaga ccgaaggcca gttgaacttc ccgtccggcg tggccgtgga caaggaaggg
    35041 gcggtgtacg tcgtcgatag cggtaaccat cgggtcatca aatacgtgcc gaccgatgaa
    35101 gagctgaacc gcggcaaggc ggaacgcgaa cgggcgcaag ccgtcagtga gtacccgatt
    35161 ccccgcaatc tggcgatcaa gcccggagat acagaaacat tcttgagctg gatggatgta
    35221 cctggggcgg tctcctacaa tctctacttc catacctcgg cgaatctgac gcaggaaggc
    35281 ggcactaaga tcgagggtgt gaccagccca tacaatcact cgggattgac caacgaccag
    35341 gcctattatt atgcgctgac ggcggtctat gaggacggca cggagagtgg attgtcggac
    35401 gaagtgtccg cgacgccggt gctgatcgat atcacggctc cgcagacgcc gtatgcggtg
    35461 atcaaccacg gcgcgtttat gacgaactcg cctgaaattg tcgtcacgat ttccgccacc
    35521 gacctcgata ccggcgtggc ggcgtactac atctcagaga acccgatgac cccgatggcg
    35581 ggaaccccgg gctgggtgga agttccgccg gcgatcaagt tcggcgcgac gattcccttc
    35641 attctgtctc ctggagacgg gcagaagacc gtcatcgtct ggttcaaaga cctcggaaac
    35701 aatatctcca cgccggcgag tgcgacgatt ctcgtcaaca cctccggcta tctttgtgtc
    35761 tccaagtggg gcaagcctgg tcgaggcgcg tcgttgttgc acggcggtga attcatggcg
    35821 ccgatgtatg ggctggccat cgatcagcag gggtcgatct ttgtcgtgga caacgggaac
    35881 aaccgtatcc agaaattcga tcgcacgggg aatttcatca ttctctgggg gaatttcggc
    35941 gccgccaatg cgaacttcca taacccgacg ggtattgcct gcgacgccaa gggcgatgtc
    36001 tacgtggtgg ataccaacaa tcaccgcgtg cagaagttcg acgggaagct gggcggctat
    36061 ttgatgaagt tcggatcgcg agggaacggc gaaggccagt tcaatgcccc atggggtatc
    36121 gcgatcgatc gggtgcgcgg atacatctat gtcgtggata gcgccaactt cagagtgcag
    36181 aagttcgaca tgagcggaga gttcatcatg tcatggggca gtttcggcaa cggcgacggt
    36241 caattctatt tccctcgcgg gatcgccgta gatcaggccg acggcacggt gtatgtcgtc
    36301 gatatgggta atcaccgcgt gcagaagttc gataccagca ccaacgtctt gccgcagttg
    36361 ctcaccaagt ggggcggcag ttccgaggcc ggccatgcca gcagcccgtt ggcgcaggaa
    36421 gccggacagt tgcgctctcc ttggggcatt gccgtcgatg gtcagggcga tgtgtacgtc
    36481 acggataccg gcaaccatcg tgtcgagaag ttcgaccgtg agggcaactt tatcgctcag
    36541 tggggtggat tcggcggtgg tgacggacaa ttcaacttcc cgtacggcat cgtggtggat
    36601 tcccggggga gtgtgttcgc cgttgatagc ggaaacaccc gggtgcagca attcatgccg
    36661 gcggatgaag gcagcgagcg gttgcaggaa gaggcggatg ccgccgcaga gttagggcag
    36721 gtcgataaaa ccacgagagt gtaggggcaa cgggcgaaag gcccgggaga gggtggcgac
    36781 tgatgttaga gaaggagatt cggctcgggc tcacctatga cgacgtcgtc ttggtaccgg
    36841 ctaaatcaca ggtcctcccg agcgaggtcg acacccgtac tcgcctgtct cgaaacattc
    36901 agctgaatat tcccatcgtc agcgcggcca tggacaccgt gactgaggcg cgattggcca
    36961 ttgcaatggc gcaagagggt ggaatcggca tcgtgcatcg tgtgctctct cctaccgatc
    37021 aggcggctga aatcgacaag gtgaagaaat ctgagagcgg gatgatcctg gatcccatta
    37081 cgatttcgcc ggatcaaacc atccgtgatg cacatgacct catggccaga taccggattt
    37141 ccggtatccc tgtgaccaag tcgggtaaat tggtcgggat cctcacgaat cgagacttgc
    37201 gttttgagac gcgaatggaa ctgaaggtgt cgcaggtgat gaagcgcgat aagctcatca
    37261 ccgctccgga gggaaccagc ctcgaaaagg cccgggagat tctgcacgag caccgaattg
    37321 aaaagctgcc ggtcgtcaac aagcaatttg acctcaaagg cctgatcacg attaaggaca
    37381 ttgagaaacg gatcaagtat cccaatgcct gcaaggatgc ccacggtcgt ttacgggtcg
    37441 gggcggcttt gggggtggga cccgataccg cagaccgggt ggcgttactg gtgaaggccg
    37501 gtgtcgacgt ggtggtcgtg gatacggcgc atgggcattc gcaggctgtg ctggatacgg
    37561 tcaaaatggt gagaaaagca catcctaagt tggacatcat tgccggcaac attgcgacgg
    37621 cccaggcggc caaggatttg gtcaaggccg gtgtggatgc ggtgaaagta ggcgtaggac
    37681 ctggttcgat ctgcacgaca cgcatggtgt cgggcgcagg catgccgcag ttgacggcga
    37741 ttgcagattg cgccagggca ttggcgggat cgggtgttcc ggtcatcgcc gatgggggga
    37801 ttaagtactc cggcgatatt acgaaagcct tggcggccgg tgcctcggtg gtcatgctgg
    37861 gcagtctgct ggcaggaacg gaagaggctc ccggcgaaac ggtgctgttc caggcacgta
    37921 cttacaaagt gtaccgcggc atgggttcga tcggggccat ggaacggggt ggcggcgatc
    37981 gatacggcca gggcggacga ccgacaccga agttggttcc tgaaggcatt gagggacggg
    38041 tgccctacaa aggcttgctg gctccccata tctaccagat ggtgggcggc gtgaagtccg
    38101 gcatgggcta ctgcggatgt aagaccatcc cggatttaca gcagaaggcg acgttcatcc
    38161 gccagacagt ggccggtctc cgcgaggggc acgtgcacga cgtcatcatc accaaagaag
    38221 ctcccaacta tcgcaccgat tgggaatagc ctcgcgtgaa acgtttctcg tgaagcgtat
    38281 ctcgcaattc agatcccctc actagacgga cgacgattca cgcttcacgg aaacatctcc
    38341 atggaactct ggcacgatag aatccttgtc cttgatttcg gctcccagta cacgcagctg
    38401 atcgcacggc gtatccgtga agcgcaggtc tattcgcaga tcctgccctg caccgtgccg
    38461 ctcgcgacgg tattagccta ccgtccgaag ggtatcgtgt tatccggcgg accatcgagc
    38521 gtgtacgaca aaaaggctcc ttcggtatcc aaacaactct tcgaacaggg cattcccatt
    38581 ctcgggattt gctacggaat gcagctggtg acgcatctgc agggcggcga agtggagaag
    38641 gctccgcacc gcgagtttgg ccgggctgag ctgaccctcg atgaccggtc ggatctgttc
    38701 aagggaatag ggactcgggg ctctaccgtc gtgtggatgt cgcacgggga tcgcatcgaa
    38761 cggatgccgc caggattccg ctccatcgca cacacggaca attctcccat cgccgcgatg
    38821 aagcggcacg acgccaggca gcgcatctac tgtcttcaat ttcatccgga agtggcgcac
    38881 accaccgagg gcgccacgat cctcaagaac ttcgtctacg atatctgcgg ctgcaagccg
    38941 acctggacca tgcgctccta tgttgacacg gccgttgaac agatcaggca acaggtcggg
    39001 acggagcggg tgatctgcgc cttgagcggc ggggtggatt cctctgtggc ggccgcgctc
    39061 acgcaccgtg cggtcggcga tcaactgacc tgcattttcg tcgacaacgg ggtgttgcgt
    39121 accggtgaac gtgaccaggt cgagaaaacc ttcgcgtcgc agatgcatct gaacatgcgg
    39181 atcctcgatc gcaccagtca gtttctcacg ggcctgaaga gcgtgaccga ccccgagcgg
    39241 aagcgaaaga ttatcggccg tctcttcatt aagaattttg aggtcgagtc caaaaagttg
    39301 aaaggcatca aatacctcgt gcaaggcacg ttgtatcccg atgtcatcga aagcgtgagc
    39361 ttcaagggcc cctcggcgac gatcaaaacg caccacaacg tcgggggatt gccgaccaga
    39421 atgaagttga agctggtcga gcccctgcgt gaattgttca aggacgaagt gcgggccttg
    39481 ggccaggagc tcggcctgcc ggatgagatt atctggcggc aaccctttcc cggtcccggc
    39541 ctggcgatcc gcgtgctcgg cgcagtcacc aaggaacggc tggccattct gcgcggggcg
    39601 gaaacgatcg tcgatcagga aattcgcgcc gcgggactgt atcgagagat ctggcaggcc
    39661 ttcgccgtcc tgctgccgat ccggacggtc ggtgtcatgg gtgaccagcg gacgtatgaa
    39721 tatgtcattg ccatccgcgc ggtcacgagc cttgatggca tgaccgccga ttgggccaaa
    39781 attccgagcg atgtgctggg gaagatgtcg agccggatca tcaacgaggt caagggcgtg
    39841 aatcgcgtcg tgtacgacat cagttccaag ccgccttcca cgatcgagtg ggagtaacgc
    39901 aagcgtgagg cgtcattcat aacgcgtcag gcgatggata catatccgat ctaatccctc
    39961 gaatgacgag agacgcttca cagaaagttt gaatggaagt cagactacaa aaactcatcg
    40021 ccggcaccgg actggcttcg cgacgcaaag ccgaggagtt gatcacgtcc gggcgcgtga
    40081 cgatcaatgg tagcgtggta aaggaactcg gcaccaaagt cgatcccgct cgcgaccatg
    40141 tcaaagtcga tgggaaacat cttcgggctg cgcagcccta tgtgtatttg atcctcaaca
    40201 agcccaagaa cgtcatgtcc acgctggatg atcctgaggg acgtcccacg gtgaaggacc
    40261 tcctgcacgg agtcaccgtg cgcgtgtttc ctgtcggccg cttggatttc gacagcgaag
    40321 ggctcatgtt gctcacgaat cacggcgacc tggcccaggc gctgctccat ccccgctacc
    40381 atgttccgaa aacctatttg atcaaggtca agggtgtgct cgacgatgcg aagatcgaag
    40441 ctcttgaacg aggcgtcaag ctggaagacg gcttcacggc gccggcgaag gtgaataagg
    40501 tcagcaaggc tgagagcaat tcgtggcttg aggtcacgat tcacgaagga cggaaacatc
    40561 aagtgaagcg gatggtcgag gcggtgggcc attcggtgat taagctcgtg cgtgtccgca
    40621 tgggaccgtt gcttttggga gatttggcgc cgcgagaata tcgattcctg accgaccgcg
    40681 aggcgaatcg gttacgggat ctggtggagg atcgggtggc cagagcggaa cgtccggaac
    40741 tggacgcgtc cggaaaaccg actcgatgga tgccgccgac ggagccggct ccgcctcgtc
    40801 ctcccaagcc cgagcgttca gggcgtgggc caaaagctca gcgttccgaa cgggggccgc
    40861 gatctcaacg gtctctggcc agtgcgaaac cttcacgctc aggtcgcgga accacgcggc
    40921 ggcccatgag tccaggtgcc aaatttcgtc gtcctgccaa aggggttggt tttcaacgga
    40981 ctcaggctgg tccgatcccg cagggggctg atcgtggccc tggctcgcgt ccgaccgggc
    41041 cagaccaaaa accccagcgc actcagcctg gagcaggatt tcagcggccc cggtccggtg
    41101 cgaaacctca aggccccggt cggggcatga cggttcggcg ctcgggtcct ggtcagaact
    41161 ttcaacagaa gcggcgcggc ggatcgtcag cttcgcgccc gggacaacga ggccctgctg
    41221 gaaaatcgcg gccctcaacg cggagaagag catgaagatc aggacccatc ggtgtgggga
    41281 attgaccaag gcgcaagtcg gtcagcaagt ggtgttgaac ggatgggtgc aacgtcgccg
    41341 ggatcacggc atggtgctgt tcatcgatct gcgtgatcga acagggttga cgcaagtggt
    41401 gttcaacgcc gagcggaatg cggcggtcca ccaagccgca cataccttgc ggagcgaatg
    41461 cgttgtgtcg gtgaccggcc aggtgatggc caggccggat gagtccaaga atccgaacct
    41521 gcccaccggt gagatcgaag tgttcgtgga tgccgtcgag attctcaacg aagccaagac
    41581 gccacctttc atgatcgagg acgacattga gatcaccgag tctcttcgat tgaagtaccg
    41641 ctatctggat ctcaggcgtc cgaggatgca gcggttgctg ggattgcgtc acgggatcat
    41701 gcaggcggtg cgggcattct tgaatgcgga gcagtttctg gaagtggaaa cgcccgtgtt
    41761 gaccaaaagt acgccggaag gcgctcgtga ttatctggtg cccagccgcg tgaatccggg
    41821 gatgttttat gcactgcccc agtcgccgca gctgttcaag caggtgttga tggtgagcgg
    41881 tgtggatcgg tattaccaga ttgcccgttg cttccgcgac gaagaccttc gtaacgatcg
    41941 ccagcctgaa tttacccaga ttgacctcga aatgtcgttc gtggatcgtg agcaggtcat
    42001 gacgctcatg gagcacatga tcgtctctgt cttcaaggat gccggcgggg tacagttgcc
    42061 cacgcccttt ccccggatga cctatgccga cgccatgggg cgctacgggt cggacaagcc
    42121 ggaccttcgc ttcgacatgc cgttgcatga tgtcacggcg tttgccgcga agagcgaatt
    42181 caaggtgttc aaggaggcgg cgaccaaggg cggactcgtc aaggcgctga tcgtgtccgg
    42241 aggggcctcc attgcgcgga gtcgtatcga cgcgctcggt gaaacggcga aaagttttgg
    42301 cgcgaaaggc ctggcctggt tgaaagtcac tgcggacggc cagttagaat cggtgatcgc
    42361 caaatttctc gatgccaagg cctttgccga ggccttgccg gaagcgaagc ccggcgatct
    42421 ggtgctcttc ggcgcggata aaccggccat cgtgcacgat gtcttggggc gaatccgact
    42481 gctgcttggt gaggaactga atctcatcga caaaacagcc tggaagccct tgtgggtgac
    42541 ggagttcccg ctgttggact attcgccgga agagaaacgc tatatcttca tgcacaatcc
    42601 cttcgccgcg ccgatggatg aagacctgcc gttcctcgac acggagccgt tgaaggccag
    42661 ggccaaggcc tacgacatgg tgctcaacgg gagcgaaatc ggcggtggta gcattcgcaa
    42721 tcatcgtagc gacatccagt tgcggattct ggatctgttg gggatcggca aggaacaggc
    42781 tcaagcgaag ttcggcttcc tgctggaagc gctggagtat ggcgcgcctc cgcacggcgg
    42841 cattgcgttc ggcctcgacc ggttgatcat gctgctgggt ggcgccgatt cgattcgcga
    42901 tgtcatcgcg tttcccaaga cgcaacgcgc gcaatgcccg ctgaccgaag cgccttccgg
    42961 cgtgagtgcc gagcaattga aggagttgcg catcaagttg gatctcgtcg agtaggtagg
    43021 agtcatccaa cccatggccg gcaatacgtt gggccgagtc ttcaccgtca cgtcattcgg
    43081 cgaaagccac ggaccggcga tcggctgcgt ggtggatggg tgtccgccag gccttgcgct
    43141 atccgccgaa gacattcagc gggatctcga ccggcgcaag ccgggcacct cacgccacgt
    43201 cacgcagcgg caggaatccg atacggttga aatcctttcc ggggtgttcg agggcaagac
    43261 gaccggtacg cccatcgccc tcttgatccg gaacgaagac cagcgcagcc gtgactatgg
    43321 caacctcctc gacacctttc gaccgggcca cgccgactac acctattggc agaagtacgg
    43381 gatccgcgac catcgcggcg ggggacgatc ttcggcgcgt gaaacggccg tgcgagtggc
    43441 ggcggctgcg attgccaaga agtggttgca ggaaaagcac ggtgttgtca tccgtggcta
    43501 cctcagtcaa ctcgggccga tcgaagctcc ctttcagagc tgggaaacgg tggacaccaa
    43561 tccatttttt tcggcgaatg ccgaggtggt ggggaagctt gaatcgttca tggacgagtt
    43621 gcggaaggcc ggcgattccg tgggcgcaaa gattaccgcc atggccgagc atgtgccggt
    43681 cgggtggggt gctccggtct atgcgaagct ggatgctgac ctggccggcg ccatgatgag
    43741 catcaatgcg gtcaaggcag tggagatcgg cgcagggttc gcctcggtcg cgcagcgggg
    43801 ttcggagcat ggcgatgaac tcacgcctga agggtttgtg acgaatcatg ccggcggcat
    43861 tctcggcggt atttccaccg ggcaggatgt ggtcgtgacc atcgccatca agccgacatc
    43921 aagcatccgt attccgcgac gttccatcga caagcagggc aacccggtga ccgtcgaaac
    43981 caatgggcgt cacgatcctt gcgtcggtat ccgggccacg ccgattgctg aagccatgat
    44041 ggccctggtg ctgatggatc acgcactttt gcatcgcgcg caaaacgccg atgtgcagac
    44101 ggcgacgccg aagattccag gttcttcaac gcatggtgct gttccggcga gtaagaaacc
    44161 aaccgcatag atcaaggttg attctgggaa gccgagtcgt cttcggtctg actcgtttgt
    44221 ttgatcggcg ggctaggggg cgttcgttat acctgatgat gaacagtaga ggactctgag
    44281 ccaatgatcg aaatttatac cgacggggcc tgcagcggga acccaggacc tggtggctgg
    44341 ggggctttac tgcgcataga caacgccgag acggaattgt gcggcggaaa tccggccacg
    44401 acgaacaacc gaatggaact gcttgccgtc attgaggcgt tgcagtcact cacgcagccg
    44461 gtggaggcgc gcgtctacac cgattcgcag tatgtgcaaa aggggatcag cgaatggatt
    44521 catagctgga agcggcgcgg gtggaaaact gccggcaaag agccggtgaa gaatgaggat
    44581 ttgtggcgcc gactcgatac gctcgcttcc ggtcacaagc tggaatggca ctgggtgcgg
    44641 ggtcacaacg gtcatccgga gaacgaacgg gtggacgcac tggcacgtgc cgggcttgaa
    44701 cagtctcgcc gggccgggaa gacggtcggc ggtacgcgac agggcctgtt ctagtgaata
    44761 tgcactagct tacgcagtca gcatccaacc tgctgtatgg cgatcccttt cccgattctc
    44821 gacattcaac atgccacggt gtaccggggc gagacccgcg tcttttccga tttctccttt
    44881 cggctcaatg cgggcgaaca tgcggccatt ctcggcccca atggggcagg caagtctacg
    44941 ctcctcaagt tgttatccgg tgaagttcat cctcttgcct tagaggagac gcgtacgcgg
    45001 ctcttcggtg aggaacgctg gagcatgtgg gatgtgcgca agaaactggg catcgtctct
    45061 tacgatctgc agcgggatta tttgatttgt gccgaggggc gcaatgtcgt cctctcaggc
    45121 ttttacgcca gtaacgacac ttacgaccat cagacgttta cgcgagacca ggtaagccgg
    45181 gcagatgcgg tgatgcggga gctaggcatc gaatcgttcg ccacgcgtgt cttcgggcag
    45241 ctctcgacag gggagcagcg gcggtttctt cttgggcgag ccttggttca cgacccgccg
    45301 gttcttgtcc tggatgaacc gaccagcggg ctcgatctca aatcctgctt ccagtacttg
    45361 gacctcgtgc gcacacagat tcataaaggc aagaccgttg tgcttgtgac ccaccatctg
    45421 cacgaaatcc caccggagat cgatcgcgtg atctttctga aggggggcaa aatactggcc
    45481 gacggcccga aactaaacct gctgaccgac gaccgcatca gcaccctttt tgaccgtccc
    45541 atcgtgctcg cccaagccaa cggctggtat caagccctgc ctggcctgtc gtaacaggtt
    45601 ggatcatcca cctgccagcg ttaccgtaaa ggtcagattg gtatcgatgc ggcggacctt
    45661 gacgctgact tgttgcccgg gcttggtccg ttcgatcaaa tagttcttta aatgtgcggc
    45721 atcctgaata tcggtgtcat cgacggcaat gatgatgtcg tgtttttcaa tgcccgcgga
    45781 cttggccggt tccatcacgt ctttcaccgc catgcgccac ctggtgccgt ctttcactgc
    45841 gaccatgtga atgcccatct tcgagaaggc catgggcttg ccttccagag cggcggtgac
    45901 gatccgttca gccagcatcg cgggaatggc aaaggccatg cggctgcaat gcgcggtcga
    45961 gtcgtcccgt tcggtctgga tgatggcgtg catgatgccg accacttcac ccttgtcgtt
    46021 gaaaaggccg ccaccggagt tgccgctgca cgcggccacg tcggcttgaa tcaatctggt
    46081 gtccacggtc tggaggaagg tgttcgtatt gcccaggtgt ccgaacgaca tagtcgggcc
    46141 ccagcccatg gggtagccca cggtgaagac ttcttgcccc ggttgcacgt cgccggaggc
    46201 aaaggggacc gttgcgcgca acttggcgcg atgggcttca gcgacgcgat agaccacgac
    46261 gtccatgaac gcgctgtcgc cgacaagatc ggcggggatt tcatggaggt cggtggtcag
    46321 gacgtggatc tgtttttgga tgacggtgcc ggtcgtgaca tcttgctttt ctgctgcgtg
    46381 gcgggcggtg acaatgtagc cgtcgcggag atgaaagccg gttccacgca ccagaatctt
    46441 gcccggttta tccggtgtgc gttggtcttg cgtgtcctcc aggacaccga tggtggccag
    46501 tttggcgcgc tccagcatcg tggcctcgat ggctgccgcc tcgttcgtga agaggcctgt
    46561 ggccagactc acagcggcca tcataccgag cgaaccccgc acaatctgtt tgacgtaatc
    46621 gagtgacgaa cgcgacatag ggaaccgttc ctttcgtgag gatgtcgtca gagctgcagt
    46681 atagcctaga ggccggagcg gatgaaatgt ggtcgtgcgt cagtgatggc gaacgtcagg
    46741 tcgaggggag attccgccgt ggcatcctct tgcgacgggc gctatactga gctcatgctg
    46801 ctaggcacaa gacagtatct gatactaggc ctgctcgctg tggccgccgg ttgcgcgacg
    46861 tcggtcattc ccgaatcgct tgagccgcaa gtcgataaga ccgtgacgtt cagtcaggtc
    46921 ttggaagccc ccgattctta tcgcgggaag gtcgtagtgt gggccggtga agtgctcaag
    46981 gcgaaagcca tgaagggtgg tactcaactg gaggtgctgc agctcccgct cgatgacgag
    47041 caggggccgg taaccgaccg catggagtcg aaagggcggt tcctggcgct tcagaaagag
    47101 ttcctcgatc cggccacgat gacggacggc accagggtga cgattgtcgg cgaaattacg
    47161 ggcgcgtcgg tcgaaaaaat agatgaggcg gactatcgtt ttccgacgct ggatgtgaag
    47221 catctgcatc gatgggattc caggcaagtg gatgaccgcc gggtgatcgg accttggtgg
    47281 aacgtgttcg gcggcgtcgg tttcgggggc ggaagccggt cgggcggcgg gatcagtatc
    47341 gggttttgat gaccagccgg tttttgggga ttccccataa atatgtcgtc agtggctgcg
    47401 ccggctgaga ggagatagcc ggtcaggaac tcgttttgct cacgaagcgt ggcggaacta
    47461 ggtgcgcccg ccggagaagg tatcacagtt gttgggatcg ccggactgga gaccgcgccg
    47521 caaccaggtc atgcgttgtt cggatgaccc gtgggtccag ctttccggct gtacgtggcc
    47581 ttgccccatc ttctgaagcc ggtcatcacc gattgcggcg gcagctcgca gcccttcctc
    47641 gaaatcgcct ggttcgatca ggtttcggtt gtgctgggca tggtagctcc atacgccagc
    47701 aaaacaatcg gcctgcaatt ccatgcgcac ggagagcgca ttatgctctg ccgtcgagcc
    47761 acgttgttga agtcgggtga ctttccccgc aatgccgagc aggttttgga catggtgccc
    47821 gatctcgtgc gcgatgacat aggcctgggc aaagtcgccg ggcgcgccga gtcgctgggc
    47881 catttcgtca aagtcgctgg gccatttcgt caaagaagga aagatccaga tagaccttct
    47941 gatcgccggg acagtagaac gggcccacgg cggacgaggc ggtgccgcag gcggatcgta
    48001 ctgcgccggt gaagaggacc atctgaggat cttcatacgt actgcccaat cgagggagca
    48061 gaactcgcca ggtgtcttcg gtgtcggcca gaaccacgga ggcaaatttg ccgaggctgt
    48121 cacgcggtgc acctttcggg ctcgggcgct ccggttcgga tggcgcggtg atttcctgga
    48181 cgccgttgag caagttgagc agcgtcaggg gattggttcc ggtcaggaag ctcacggcga
    48241 ggaccaggac gatgccccct aatccaaggc ctacgccgga cctggccgca cccatccctc
    48301 gacgatcttc aatgttccgg ctctcgcgtt gaccttccca tcgcatggca ggactccttg
    48361 gtgaatatca ggctcgtaac gtctcgtcga ggactatgag ggcacgctga tggaaccaca
    48421 tttgtcatac ttgagcaagc ctggggtgtc cctaacgcgc aatactcata agccgttcgg
    48481 cgattgtggt gctggcagtg ggcgtgagtg cagggcctca gcctgcgcct ccgtgctggg
    48541 cggtagccga ttgtctcacg ttgacgcacc ggattggttg tccagaagag cctgcataat
    48601 cggactagac aggccaacgg tcgcttcgct agactcattc catgaccact gtgaccaacc
    48661 cgatgcatcg acaagcccca actcgggcgc gtttcattgt tctggcgttg gcggggctgc
    48721 ttagcctctc cggctgtcag tcaccgggaa cgccgctccc tccgaccttg aagccgagcc
    48781 tggcgtggcc ggaatgtccc aagatcggcg gaacgtatga gcttcaggga gagccactcc
    48841 ccggcacgat gaatttttac cggaaccggg acaacaagct caccttgaat gccatgctgg
    48901 gcgtggcggt gcctgcggat ctcgcgcagg aggccgtgcg cgtggaattg gtccatgagg
    48961 acaccctgga actggtcaac cggtttgatg gcgccatcac ggcccatcaa ttggatctac
    49021 agccggagga tcgggtcacc tgcgatcgca atcagatccg aattcaccgg acccgctcgg
    49081 aaggcggggt agccgaagag cccctccgca acatttccga catcgcacag gaattgactc
    49141 tggaagggga cggaagtctg ctcgtcaaga cccgcatcat gaatcagagt aaatccgcct
    49201 tcttcagcac accggttcct catgaggaat actccgcgcg cttccggcga ctcgattagt
    49261 cgcggtcccg cgataggtcc ctcgttactg gtcattcgtc acacgcactc cttcatcatg
    49321 ttgcggtgct gtccgtccac ttctgtgatg aggcgaagga ttgcggcgat acggagagtc
    49381 ccgttctgat gacggttcac cgcactgccc gcacgctggt gttcaacaag ccctacgatg
    49441 tgctgccttg ctttaccgat ccggaggggc gtcagaccct gggcgactac atcgatgtgt
    49501 ctggcgtcta tgcggccggt cgattggatc gggatagcga aggcctgctg ctgctgactt
    49561 cggacggggc gttagcccat cgcattaccg atcccgagca ccatcttccc aaagtctatc
    49621 ttgtacaggt tgagcgtatc cccgatgcgg ctgccgtgga acggctgcaa caaggggtgg
    49681 tggtcggcgg gagacgcacg aagcctgctc gggttcgtct gttgctggaa gcgcctcaga
    49741 tcccggagcg gccggtgccc attcgattcc gaaaacatgt gcccaccgca tggttggagc
    49801 tcaccctgcg cgaaggcatg aatcgccagg ttcgacggat gacggcagcg gtaggccatc
    49861 cgacattgcg actggtgcgg gttgcgatcg gcccgatttt cttgggggcg ctcctgcccg
    49921 gtgagtggcg agaacttacg aaagcagaga cagctgagat ttatggttat tcctgagaga
    49981 gggcgtacct gatggcaggg cgttaacccg gattattcat gaatacctgc cccggcgtcc
    50041 gagacaagcc tcgccgggcg agaggatcgg acgccggggc aggtattcat gaatagtccg
    50101 ggttaaggag gcagctcaca tgaatactcg tgatcggatc atcgtcggtc tgtgtagtgt
    50161 gctgctggga ctgctggttc ttgtttcccc ggctcatgcc gacgacacgc ttgtgacaaa
    50221 gcaaagtcac tatacggttg ctgagacgat cgatcggatc gcacgggccg tcaccgacaa
    50281 gggaatgctt ctcttcgctc gcatcaatca tgcggacgag gcgcggaagg tcgggtttga
    50341 aatgccccct acggaattac tgctctttgg taatcccaga ggcggcacgg cgctcatgct
    50401 ggcggctccc acggtggcca tcgatttgcc gatgaaggcc ttggcatggc aagaccaggc
    50461 cggccgggtg tggctcacgt acaatgctcc tacgcttttg ccggtgcggc acggattagc
    50521 ggcagagttg gcggccagac tcgatccggt tgggaccctg ctggagcggg cggtggagta
    50581 gtggcgggat tgttgagccg tgcgttggaa aaagagttga agaggattaa gatgggccgc
    50641 attgtcgctg aatcatggag tgatgctgaa gttccagagc atggattgat cggaaggatc
    50701 tcagatgagg gcgtgaggag ggtggaagcg ttgaatgctg tgagtcgaac gaggggagtg
    50761 acgatgaggt cacagacaat cgccgtgggc agggtgtcct tgttgctggt gctgtgtggg
    50821 atcggtcttc tgccgaccgt atcggcgctg ggtgccaatc cggagtccgt gccattgtcc
    50881 gatatgcgac acattaaaaa gttcgtggcg gttgaagtgc aaacccaggg aacggctgag
    50941 aagcttggca tcaatggagc ggagttgacc gatgtgacga gggcgacgct gctcaataag
    51001 gtgccgggga ttgcgctcga atcgtccagt ggtccttctt ccgacgcttc cgagcggctc
    51061 aaccagcttg gattttttac ctgcgaggtc tggacggtgg gcgaacagta tattgccgcc
    51121 tatcacgtag actgtaacgc ggggtcgtac actgcacaaa aaacgccggg gagcctgtgg
    51181 aatcaagcga ttctcgggta cgggccgagg gacgaagtct ccgatgccgt ccgaaaaggg
    51241 gtgcgtgcga tggtagagct ctttgcaaac acctttgcta atgcccgtat ggacgccggc
    51301 cggtagggcc ggcgtgtgcg caggcccagt cttaccccat cgatgcgaac gatggagcgc
    51361 atggcgtctc cctgtgcgac cgttctcctt ctctccgacg atcctgcatg tcctgcactc
    51421 attcgcacct ctggaccagc ccccaaaccc taggttgaat cctctgaaga atctcttttt
    51481 tcttgtctgc acttggatgc gacatagaat aaggggggta tggctgatgc atcgtcaggc
    51541 cggcgaatct ctcaccccta tggttggctg ctactcgggc tgctgcttct gttcttcttc
    51601 cgtgtggcgg cacagctgat tcaagcctac tcgccggtta cctcgctgcc gccattcgaa
    51661 acctggcaga gcggagcgct tccgtatccg ctgctcgttt cgctccaatt gatcattctt
    51721 gtcgcttgcg gtcgtgtcgt gtggagtttt ctgtccggga ccgtggtccc ttcagggaaa
    51781 aagggccggc tgctcctggt gctggggtgg gtctacttcg tcgtgatggg gattcggctg
    51841 ttgattgggc tcaccgtcgc accaaatcat ttttggttcg gcgccattgt accgacgtta
    51901 tttcatctgg tgctggctag tttcctgctc gtcagtggct cctttcatac ggcaagcgga
    51961 tcctgttcgg tcgcaccagt ctcggaaggt gcggcgtgat ccttctgctt cgatatgggg
    52021 cctatccctc aatcttgctg gcctgcgtaa tcggcaatct tgtgctgttt cacatgggca
    52081 cgggggtgct gccggcgacc tatctgccgg tcacgctcgg gacgttgctg atcatggcgc
    52141 tggaggcgac gttgccctat cgatctgact ggcggccgtc gcgggttgag gtcatccagg
    52201 attcgacctt tctggcactc gtgcatatca tcctgccgaa ggccttggcg gcggcgtcgg
    52261 tggttgtgat ctttgatgtt ctccacggac ggggctgggg tatgtctgtg tggtggccgc
    52321 atgactggcc ggttccgacg cagacgatgt tgatggtgct gatcgtcgat ggcctgcgct
    52381 attggctgca tcggctcagc catgagtggg agccgctctg gcgcgttcac gcggtgcatc
    52441 atgcgccgca gcggctctat acgctcaacg tgggacgatt tcatcccatc gacaagggcc
    52501 tccaatttgt cttcgatgcg ctgccgttcc tcgcgcttgg cgtccaggaa gacgtgttga
    52561 gcctgtactt cgtgtggtac gccgtgaacg ggttcttcca gcattccaac gcggatgtgc
    52621 ggctgggatg gctgaattat gtggtcagtg gaccggaact gcatcgatgg catcattcca
    52681 tgcagaagga ggagtcgaat cacaattacg gcaatcattt gattgtctgg gatgttgtgt
    52741 tcgggtcccg ttacctgcca gctgacgggt cggtgggtgc gctaggcttg gtgaatcggc
    52801 agtaccctcg tggttttcta gatcaaatgg ctgcgccatt tacccccggc cttgataagc
    52861 agacagtgtg agagccggga tgttgagatt tctccatgat cctgccttgt cgctctcact
    52921 gacccttacg aagtatcgcg attggaatcc gtttattgcc catacccatt gcccggagct
    52981 ggctcagcag gctgcgcttc gggatattct ggcgcgacaa gccggcacgg cctttgggcg
    53041 caagcatcgg ttcgggtcct tgcgcaggta cgaagagttt gcctgcgagg tgccggtgtg
    53101 tacgtacgaa gacctgcggc ctgccattga ggtgcaggaa aagagcaggg agccgcttct
    53161 tacctccggc cagcctatcc tctttgcgca aactagcggg acgacgggag tgccgaagaa
    53221 catcccgatc ctcccgcaaa cggtcgaagc gatacgccgt tatcagggcc tgtgtgccta
    53281 tgcgcaatgg cagggagtgc cggccatcta ccaaggtcgc gtgctggtga tcagcgggca
    53341 aaccatcgag ggccacttgc ctggcggcac cccgttcgga tcgatgtcgg gcttgatgtt
    53401 cgattgtttg ccagccgcca ttcggagaaa aagcatactt cggggcggcg aatccgcagc
    53461 gtcggattac cggcagcgct atttcaatat cgcagtccga gcgctggcgg attcctctct
    53521 ttcagtgctg gccacaccta atccgtcgac gattctgaaa ttgctggagg tgattcgcgg
    53581 ggagtatgcg ctactgctgg aggcgttgtc cggcgagacg cgagggggtt gtctgccgcc
    53641 atttgagaag cttccaatca gttcaacgag attgtcacag cttcgggtgt tcatcgggca
    53701 agaggaggcc ctggattgtg cgacgctctg gccgaacttg cgggccgtgg tgacgtggac
    53761 tggtggaaat tgtgcggtac tgatacccaa actagggtcg ctgttgtcgc cacagaccag
    53821 gattattgaa atgggctatc tctcgagcga atgtttgggg acggtgaatg tggacgtgct
    53881 caacaaccga tgtgtgccga ccctagcgga taatttgttt gagtttgtgg aggttggaga
    53941 cgaagcatca gatgttgaac cggtcctgct ccagcagctg caggcgggcc ggaaatacac
    54001 cgtgattgtg acaaccagga acggtctgta ccggtatgcg atgaacgata tcattgaagt
    54061 gacggggtac ttcaatcgga cgcccacgat ccgctttgtc caaaaaggga agggtgtgac
    54121 caatatcacg ggggaaaagc tctatgaaca tcaggtcacc gaggccgtgg ggcaggttct
    54181 gatcgcgcgt gggctgagct cggaattttt tgtcatgttg gccgacgtcg agaattctcg
    54241 ctacaccctg tgtctagaac atgcctcgtc actgggtgat ctgggaatcc tcgtggagga
    54301 gcggctctcg tcgatgaata tcgaattcaa ggccaagcgt gcaagtgggc ggctgcagcc
    54361 gattcacgtc ctccagttgc gtcctggaac cggagatgtc tatcggcaac attgcgtcag
    54421 caaggggcag cgcgaggcgc agttcaagct cattcgcctc caatacgccc atgattccac
    54481 cttcgatttt gagcggcatg gggaggtgat gacgtgatgc gcatgcgtag cttgaccatg
    54541 acatccttag agatcccctt ccggcaggtc tttacgcatg cctcggctac acgagccaag
    54601 accgaggcgg tgctggttcg cgcagagtct gctcgtggct tggtggggat gggagaaggt
    54661 tgtccgcggc agtacgtaac gggcgaaacc gtggctagtg cccaggagtt tttccgcagc
    54721 caccgggccg aatggatgac atgttcatcg atggatgatc tgcagacgtg gggaaccgcg
    54781 catgctgatc tgatcgaccg caatccggcg gcctggtgcg cggttgaaac ggcctgcctg
    54841 gatttattgg gaaaagagct ggaacagccg attgaagtgg tgcttggcgg cacgccgttg
    54901 tccgggccat ttcggtactc ggcggtacta ggcggggaaa agttttcctc gttcgagaaa
    54961 cagctgcgac agtatcttgc cgcagggttt atcgatttca agctcaaggt catgggcgat
    55021 ctggaggccg atcgtgagcg tgtagcggcg ctcacggctg cgggcacacc aggccttcgc
    55081 gtgcggcttg atgccaacaa tcgctggcac cgcgtggatg aagcctgcga gtatattcag
    55141 agcttacagg gctcatttac cgctctggaa gagccgctgc gggtcggcga ttacgagggg
    55201 tgtcgggcac tctcccggca ctgtggcgtg ccgattattc tggacgagag ttttgtgaat
    55261 gtgggtcaat ttccgttgat tgaaggcgag ccttccttgt ggattctaaa tattcgcgtc
    55321 tccaagatgg gcggtctgct aagagcatgc acggtcgccg cgcgtgcgaa ggcggcaggt
    55381 attccgatcg ttgttggcgc gcaagtaggg gagactagca tcctcaccag agtagcgctg
    55441 gttgtggcgg accggtaccg cgacatcctg atcgcgcagg agggggcagt ggggacgcat
    55501 ttgcttgaat atgatctgtg cgagccgccg cttatgttcg gcagaggcgg ctgtcttgcg
    55561 gataccgtct tttacggccg ctctggtctt ggattgtcgt ttgcgcggta agattgccga
    55621 tgtcgggaat tggacgtgcg tatgagtgta gaactacgta cttaaacgac tgctcggtta
    55681 gaggctttcc acgtccgcaa caatcgtgtg tagtccgctc gatccggccg gctgcggtcg
    55741 aatgagttcg gcgatctgag gttgcccttt gctatcgatc gcgcgaacgg cgacctggta
    55801 cttgccccgt ttcgaggggc gccaagtgta gttccaaatg acccatgaat agggcgacat
    55861 cggcgtttca atctcgcagg aatcccacgt tctcgtggca tccaggctga tttccactcg
    55921 gctgatggag tgtgggccgc caaaggcaat tccgcggaat cgctgctcgg ggccgcgtag
    55981 cgtctggtag tgtccggggg agtcaatgcg tgaaaatgtc ttgatggtgg cgtcatcagt
    56041 ccaccctttt cgttgccaat aacctcgata atccccggcg taggcctcga tttccacaat
    56101 ccatttcaca ttcttgatgc cgtagagtcc aggtaccaat aggcgcagcg gaaagccgtg
    56161 ttccttcggt aacttctcgc cgttcatgag aaacgcgagc atcacatcat cctgcatggc
    56221 gcgcgtcagc ggaatgctat cgtcgtaggc gtccgctccg cggaagacaa tgtcacgtgt
    56281 ggtttcttca tcgatctccg cttcctgtag gagtttcttg agggaaatcc ccctccagcg
    56341 tgcgttcccg aggctatcgc ctccgggtaa tgtatcgatg cacatgagag tagagatctg
    56401 ctcgaagtta ttgcggttta atagctctcg ccagccaaga gacaggggtt tcttcacttc
    56461 tcccttcacc gagaggcgcc attcgtcttg tcgaagctcg cgagagacgt taaaagggga
    56521 gtctgagtaa ttcaccacgt agaatttttt gttttctgtg aagtaatttg tctcacgcgg
    56581 aggcatggcg aacatgcgac cgaataaaga tccgaccgca tcgcagccgc cggtgagacc
    56641 agcaacagcc gcaattccag tgagctttaa gagatttcgt cgtttcagcg agagccaatt
    56701 ttccatggaa atactctgcg acaataaaaa gatcttttca aggcttgact ccagaaaccc
    56761 agattggtat aaggatgcac gtttgatcca gcctagtggc cgatgcggtt gtaagaccag
    56821 cgacggattc tcaggaaccc cagatcaaaa aaataaatcc tgatcctctt gacaaggtca
    56881 ggcgcgcata gtaaagtgcg cggctcgcgt ggggaacgac aaagcgttcc gaagcgtgaa
    56941 acggttcttt gacaactgaa tagagcgaga aatgagtaag gtaaggtgta ttgaagaggt
    57001 ggccctcggg tccaattcaa gtagaacctt tatgagagtt tgatcctggc tcagaacgaa
    57061 cgctggcggc gcgcctaata catgcaagtc gagcgagaag gtgtagcaat acacttgtaa
    57121 agcggcgaac gggtgaggaa tacatgggta atctaccatc gagtggggaa taaccagccg
    57181 aaaggttggc taataccgcg tacgcctccg gatcttcggg ttcggaggga aagccacact
    57241 gtgagtgcgg cgctcattga tgagctcatg tcctatcagc ttgttggtag ggtaacggcc
    57301 taccaaggct ttgacgggta gctggtctga gaggacgatc agccacactg gcactgcgac
    57361 acgggccaga ctcctacggg aggcagcagt aaggaatatt gcgcaatggg cgaaagcctg
    57421 acgcagcgac gccgcgtggg ggatgaaggt cttcggattg taaacccctt tcgggaggga
    57481 agatggagcg agcaatcgtt cggacggtac ctccagaagc agccacggcc aacttcgtgc
    57541 cagcagccgc ggtaatacga aggtggcaag cgttgttcgg attcactggg cgtacagggt
    57601 gtgtaggcgg tttggtaagc cttctgttaa agcttcgggc ccaacccgga aagcgcagag
    57661 ggtactgcca ggctagaggg tgggagagga gcgcggaatt cccggtgtag cggtgaaatg
    57721 cgtagagatc gggaggaagg ccggtggcga aggcggcgct ctggaacata cctgacgctg
    57781 agacacgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccctaaact
    57841 atggatacta agtgtcggcg ggttaccgcc ggtgccgcag ctaacgcatt aagtatcccg
    57901 cctgggaagt acggccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg
    57961 gtggagcatg tggtttaatt cgacgcaacg cgaagaacct tacccaggtt ggacatgcac
    58021 gtagtagaaa ggtgaaagcc tgacgaggta gcaataccag cgtgctcagg tgctgcatgg
    58081 ctgtcgtcag ctcgtgccgt gaggtgttgg gttaagtccc gcaacgagcg caacccctgc
    58141 tttcagttgc taccgggtca tgccgagcac tctgaaagga ctgcccagga taacggggag
    58201 gaaggtgggg atgacgtcaa gtcagcatgg cctttatgcc tggggccaca cacgtgctac
    58261 aatggccggt acaaagcgct gcaaacctgc gagggggagc caatcgcaaa aaaccggcct
    58321 cagttcagat tgaggtctgc aactcgacct catgaaggcg gaatcgctag taatcgcgga
    58381 tcagcacgcc gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgaa
    58441 agcctgttgt acctgaagtc gcccaagcca accgcaagga ggcaggcgcc cacggtatgg
    58501 ccggtgattg gggtgaagtc gtaacaaggt agccgtaggg gaacctgcgg ctggatcacc
    58561 tcctttctaa ggagtaccac tccttctgaa aaaactagga tcttgagggt caccttttca
    58621 atacactaga cacggtcccg aaccgcatca tccaggtgag tattatcact gttggctcac
    58681 gagatgtagt gccacacgag agtttatgat tgtcttgggg tggctcagca gtcggctggg
    58741 cctgtagctc agctggctag agcacgcgct tgataagcgc ggggtcgata gttcgatcct
    58801 atccaggccc accaactgag cctcgagtgt gagtatctcc ctcggcggct caaaattgac
    58861 aatcattcat ttgtgagcgg ggctgtagct cagttgggag agcacctgct ttgcaagcag
    58921 ggggtcgctg gttcgattcc agtcagctcc accaagggtt tgtactgcat tcatttctcg
    58981 ctctattcgg tcgtggatgg cacgcgatgt catcccagtt ctttgaaaat ttaataggtc
    59041 tgagaagacc atgtgtatca ggagcaaaga gaattgttaa gctactaagg gtgtacggtg
    59101 aatgccctgg catcagaagg cgatgaagga cgtagctagc tgcgataagc cccggggagc
    59161 cgcaagcagg ccgtgatccg aggatgtccg aatggggaaa cccaggcgat gaatgccgcc
    59221 tatcctctgc tgaatacata ggcagtgaga agcccacgca gggaagtgaa acatctcagt
    59281 acctgcagga aaaaaaatca atcgagattc ccccagtagt ggcgagcgaa aagggaacag
    59341 cctaaactat gccgatgtca agcccgtgtg cgttgtcggt gtagtgttgt aggcctccgt
    59401 tggacggtga tacggaaccg tcggcgagtt acaaattagg atcgtaacag aatagtctgg
    59461 aaaggctgac catagaaggt gacagtcctg tatgtgaaac gatcctaact cgttgaatgg
    59521 aggtcctgag taccacgggg cccgagaaac cttgtgggaa tctgggacga ccacgttcta
    59581 aggctaaata ctctctgatg accgatagtg aaccagtacc gtgagggaaa ggtgaaaaga
    59641 accccggtga ggggagtgaa atagaacctg aaaccgtaca cctacaagca gcgaaagggc
    59701 tatgcccgca agggaatgcc tgatcgtgtg ccttttgctt aatgagcctg cgagttattt
    59761 agcgtagcaa ggttaaggcg aagagccgga gccgtagcga aagcgagtcc gaattgggca
    59821 ttgagttgcg ctgaatagac ccgaagcgaa gtgatctacc catggccaag gcgaatccgc
    59881 cgtaacaggc ggaggaggcc tgaactgatg ttagttgcaa atggctcgga tgagctgtgg
    59941 gtaggggtga aaggctaatc aaacttcgtg attgctggtt ctccccgaaa taactagagg
    60001 gttagcctcg tttcagccgt gacggaggta gagcactgaa tgagctaggg gcgttccaac
    60061 gctaccgaac tcaatcaaac tccgaatgcc gttactggac gaacgggagt tagactacga
    60121 gtgctaagat ccgtggtcaa aagggaaata gcccagaccg tcagctaagg tccctaatcg
    60181 tgagctaagt ggaaaaggtt gtaagatcgc tcagacagcc aggaggttgg cttagaagca
    60241 gccatccttt aaagagtgcg taatagctca ctggtcgagc gattttgcgc cgaaaatgta
    60301 acggggctca agttcacaac cgaagctacg gacttgcatc gcaaggtgtg agtggtaggg
    60361 gagcattctc tgtgcggtga aggttgaccg acaaggacaa ctggagcgca gagaagagat
    60421 tatgcaggca taagtagcga taaacgatgt gagaatcatc gtccccgtaa gcctaaggtt
    60481 tcctggccta tgttcgtcag gtcagggtga gtcggatcct aagccgaggt cgacagacgt
    60541 aggcgatggc aaacaggtca atattcctgt accaccgtat tgacgttatc agcgaagggg
    60601 ggacgcagaa gggtaggcac cgccgggtcc ctggaatacc cggtccaagc aggtaggcgg
    60661 gggttgtagg caaatccgca atcccgttaa cgccgagatg tgatggggag gccgcaaggc
    60721 cataaacggg ctgatcccac gctgccgaga aaagcctcgt agcgagtcaa tatgatgtcc
    60781 gtaccgcaaa ccgacacagg taggtgggtg gaatacacca aggggcgtga gagaacactc
    60841 cctaaggaac tatgcaattt agccccgtaa cttcgggaga aggggtgcca cgagtaggta
    60901 agtctgtaca aggcaagctg aacgtggttg caataaagtg gctctagcga ctgtttacca
    60961 aaaacacagg actctgcaaa cacgatagtg gatgtatagg gtctgacgcc tgcccggtgc
    61021 tggaaggtta accggaggag ttagccgcaa ggcaaagctt tgaagggaag ccccagtaaa
    61081 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
    61141 ctgcatgaat ggcgtaacga ctggagcgct gtctcaggga gtgactcagc gaatttgttg
    61201 ttccggtgaa gaagccgggt gcccgcaact agacggaaag accctgtgca cctttactac
    61261 accttgacat tgggtgttgg aaggatctgt gtaggatagg tgggaggctg tgagacttgg
    61321 ccgctaggtc aggtggagcc gacgttgaaa taccaccctg attgttctga tattctaacc
    61381 tggccctgtg atccagggca gagacagtgt ctggcgggta gtttgactgg ggcggtcgcc
    61441 tcccaaaagg taacggaggc gcccaaaggt tccctcaggc tggtcggtaa tcagccgtcg
    61501 agtgtaaagg cagaagggag cttgactgcg agagcgacag ttcgagcaga gacgaaagtc
    61561 gggcttagtg atccggtggt tctgcgtgga agggccatcg ctcaacggat aaaaggtacg
    61621 ccggggataa caggctgatc tcccccaaga gttcacatcg acggggaggt ttggcacctc
    61681 gatgtcgact catcgcatcc tggggctgaa gctggtccca agggttaggc tgttcgccta
    61741 ttaaagcggt acgagagttg ggttcagaac gtcgtgagac agttcggtcc ctatctgttg
    61801 tgggcggagg agagttgaga ggggcgttca ctagtacgag aggactgtga aggatgaacc
    61861 tctggtgtgc cggttgtcgt gccaacggca gtgccgggta gctatgttca gttgggataa
    61921 ccgctgaaag catctaagcg ggaagcctgc ctcaagataa gctctcccgg ggcgcaagcc
    61981 ccctaaagga cacttgtaga ctacgagttt gataggctgg gtgtggaagg ctcgtaaggg
    62041 ctgtagctaa ccagtactaa tcgtccgtgc ggcttaacat cctttgctcc tgatagacgt
    62101 ggtcttaata agaacaaatg aatgacgaac tatgtcttcg tcgatcaggg acgagtccca
    62161 tatgatcgcc tagaatagga actcactggt ttgattcttc agaagttccc ggtggccttg
    62221 ccggaggggt cacacccgtt cccataccga acacggaagt taagccctcc aaggccgatg
    62281 atactgctgc cgtgaggcag tgggacagta ggacgctgcc gggttacaaa aagagcctgc
    62341 tcgagaaatc gggcaggctt ttttatttgc cattgaatct cagaaggtct aactgattaa
    62401 tgcagtcgcc gatgggtgtt tgccgaattg atttagcgct tatcagagct caatggcaat
    62461 tgaagtgtac agtgtacagc gcttttcatt tgaaattcat tgctgactaa agagtccctt
    62521 tctatgctgc cacctattcc atccagctgg taccagtttt tcacggaaca gataaatggc
    62581 aacaactttc gagctctcga aacgtttctc gatcgggaag cggaggcagg tgaaacaatc
    62641 ttgcctccct cgcaggatat ttttggggcc ttggaggcga cgccatacga acaggtcaat
    62701 gttctgcttc ttggtcagga tccctaccat acaccaggaa tggcgcacgg actctgtttt
    62761 tcggtacggc ctgacgtccc ggttttaccc cgctcgctca gaaatatcta tcgtgaactt
    62821 cgggaggatg tcggctgctc tgttccaaac aacggctgcc tggagccctg ggccaggcag
    62881 ggagtgctga tgttgaatgc cgttctgacg gttcgcgcgc atgcggccaa ttcacatcgg
    62941 gggcgtggct gggaagcagt gactgatcgt atctttgcgg tcctgaatgc aaaaccaagt
    63001 cgtgtggtat tcctgctctg gggcgcggag gcgaagaaaa aacaatcact gatcacaaat
    63061 gcgcagcatg tggtgatttc ctgcgctcac ccctcgccct tgtccgcgag aaagttcttc
    63121 ggttcacggt gtttctcgca ggtcaatcga gccctcactg aggtgcacct taaaccgatc
    63181 gattggcaga ttcccgatct ctgaatcgga gtcgggtggc gcggtagcag agatcggtgc
    63241 tatactagag gaattctttt agttcttact caatcatctg cgcaggagag tggccgccgg
    63301 gaatgaggat tcgtctgggt ctgggtctcg gttttagtac tctagttgta gcaggtacgt
    63361 tgttggcggc cgagggcgac cttgccggac tgcggacgcc aacgtctatt ggatcggcgc
    63421 ccgtcgcgtg gcagtatgat ggcgaccaag ggccctccca ttggggggaa atcgctccca
    63481 ccaccgcctc ctgtgaaaaa ggcactcacc agtcgcccat taatattcgg accactcccc
    63541 atcaccagag ccatgacggg ctcttagttc attacactgc cgctcccggt cacgttgtca
    63601 cttctcacca caccattgaa gtggattttc aatcaggcga gtcgcttgaa gtccttggac
    63661 gaacgtatac gctcaaagag tttcatttcc acgagcccag cgaacatcag ctgaacggcc
    63721 gcacttatcc gatggaggcg cacctcgtgc atcgagatga gacgggtcat ctggtggtcc
    63781 tggcggtgct gatggatttg ggtaatgaat cggcctctct gtcagccgtg tgggatcgga
    63841 ttccgagcga gaagcaggat gaggtgcggg acttgctgat taacccgcag gatctgctgc
    63901 cgaaagactt gcaccattac gcctatgatg gctcactgac cacgccccct tgtacggaag
    63961 gggtgcactg gattgtgctg aaggaaccta cctccattac ctctgcccat attgaacggt
    64021 tcgtgtcgct gattggccac aacgctcgac cggttcaatc gttgaatgag cgtgagattg
    64081 acgaagaata agctttttcg ttcagacaac gatcagattt tcagagccac ccaccgttga
    64141 ttcgggcgtt tcccgcgacc accagtccca gctttacttc gagtgtcttt cgagcaattg
    64201 tggacggtca cgtgaacgtg tgtcgaccag cctcagccac ttagtggatg ccatcctcct
    64261 gtggaggctg aggtcctgcg accatacctg ccaggtcggg gtcatcttga ccttgcgatc
    64321 ttggcccttc tgcttttcgt tttaggcggt gagcctcttt ctgcgtgcgg cgatcacgtt
    64381 gggtctgttc ccgttgccgt tttttagaag tcgtatggct catggtcacc tccgtgggtc
    64441 gcatgggagc gacgtatatc aggcggatgg cacttgaggg ggcgtcgttg ctggcacggc
    64501 gctcgatctg ccggctttga tgaatcgatc ttcggcgagc acgccgtgta gttgtgccgt
    64561 gatcgagcga cggtacggtt ccttctgccg gatcatgatc acgagattgt ccccatccac
    64621 aaggatgctg aggtcgtctt cagtaactag ttcccgggac gagccaccga tgttcaatcg
    64681 gtactgtgtt tttccatcgg gattccgatt cggtccgacc acgatctcag tcaatccgtc
    64741 gatgtggccg tcatacccct tatgccggtg cctgaccttg gtgccgtttg gaatgcgtac
    64801 ccatcgtgcc accggcgatg tctgactgtc ttgcccgttg ctcatagcgc gtgtgtctcc
    64861 ttgttatcgt acccgtacgt agccgccagg gtgctcgtga ttccgtgagc gcacaattgc
    64921 atcgcggtac acggctgggc tgacgactgc gaatttcaga atgaaacgag cggagtatcg
    64981 cggacggaag ggcggttgaa tcgatcttgc taatctacgg acacagggtc gataggaagc
    65041 aaagttaagc ggctgaggag tcagcgatcc atgcagagtg tatcacattt gttcatgccg
    65101 catcagccac gccttaggat ggaggtgctt ggttatattg tcgaggttcc gcaatcccca
    65161 tatgcaggtg tggtcatgcg cgatgctgtt gatttggagc aatcggcaca cgaatatggt
    65221 gcaaaacgac cgatcgctga taataattat tgccgcatag aatcatgctc ttgcattgag
    65281 atcggcgctc cggcacgttg acaccactct cgcagcccta tagggtgaac ccatgacgta
    65341 ctaagccacg ccggctttct cctttcgcgc gcactttctc tcccccctca ctccctgcgc
    65401 gtgcgagtct caagtcttcg ccgcttcgac gccacagaac aagagtccag cgatggtagg
    65461 cccgcagtga tcggtcattg accgatcaga cgatctactc aggatggagt cttctactta
    65521 cggcaagatt cgcgcagcga gggaggttgt catgtatcaa atttggacac tcatcggaat
    65581 catggtcttc acgtgggcgg ttgcgatgtg ggctagttac tcggacggca ctgcgtagac
    65641 agacgattcg agttacgagt gctcttgagg tctcctgcca accggcaggg atctgatgac
    65701 aaatggaggg taccatgatg gcgcagacgg tactgttctg ggccgcacac aatctgttta
    65761 cggtggcgac ggtctgcttc ggtggggcca tgttgaccgt tatgctgtac acctacctgc
    65821 atgattatgt gctggtgacg aaggggcgct agcctgtttc tgtaaggtac acggttgcct
    65881 attggggagg ttttatccta tgcgtattca tcgaacaatc accactagag tattgagtct
    65941 ttttttggca accgcttggt tgcttgccgt ctcactgacc gtccatgccg cggaacagaa
    66001 acccgttgtg accgtcgcgc cggtcaagtc cgttgcggtg gcttccggtg ccgtcgagga
    66061 tacgctgccg acttgcctga cgagaattcc tcagggggcg acacccgggc aacggttgat
    66121 tgccgaacag agttgccagc gagatgaatc cgatcgtgca tccatgcggg ctgttcctgg
    66181 acggtagccc gatcgtccca cctggctgcc tgcgcatgga tttgaccggg tgtggtgcgc
    66241 cggctgtgca cgaggcagat gtagggccat tttttcgtgg ctcggggcta agttgaaggg
    66301 tgagcaatgt aggaaggatg actgtatgcg tatggaacat cgagttcgtt caagatcggc
    66361 tggccggcct ggtgtcgtcg aggagcgaat ggtgtcaggg gcgggtgaga cgcctgcggg
    66421 agtcggtcga aatatgcttc tcgatatcaa tagggctgag atttcccgac tcaagtcgct
    66481 gaccggaatc ggcgaacatt atgcgaagaa gatcgtggaa gggcggcctt accatcatcg
    66541 agaagagttg ctgaccaagg agattttgcc ggaatacatc tacggcagaa tcatggatcg
    66601 actgatggca agccagtctt aggatttggg gatgggtgac gtgcctggca tcggatgcct
    66661 cgcgtgagat ggtcatccga tattgaataa ccaggttgtt ctctctcatg aatgggacgg
    66721 gtggctcgaa tgaccggaag gaacgactgc atgacggaca atagcaggac agcaggggcg
    66781 gcgagaaggt tctggctacg tcaggtatct tggcggggca tggccctcgt agcagccatc
    66841 agcctgctga tgggcatcgt gcaggccacg gccgcttggt ccgagcaggg tccgaccgag
    66901 gccgtgaagg ggaccgtctc cgagctgctc tctatcctca aggagctcaa agacccgact
    66961 cgatccgagg cgcggcgttg ggaaatcgaa caggtcattc ggcatcatgt gcattacgaa
    67021 gacatggcca agcggtctct gggagcatcc tgggggcaac tgagtgatgt ggcccgccgg
    67081 gagtatgtcg ggctctttgt ccagctgctt cgcgatgcgc ttgcgaaccg gatggtggag
    67141 tattccggtg aacgaatcag ctatctctca gaacaacggg agcgcacgtt cgcgcaggtc
    67201 aaaacgcggc tggtcgggaa caaggtcgac accttcatcg actttcgttt ggtcagtcag
    67261 gacggacgct ggctggtcta cgacgcagtg atggacggag ggagcttggt cgcgagctac
    67321 catgcgcagt tcgcgagtat cattcgcgat gcctcctgtg cgcagttgat ggagcgaatc
    67381 aaagagaaga cactggtcgt gaaattgttt gagaagagcg ggtcgtgagg cgaggggcgc
    67441 aatggcacgg tcacgagcgg tcatgtcccc gggagagagc gcaatgagat gtccgcgttg
    67501 ttatggtccc atgatgatga tcaaaatgaa ggatattgtg agcgggacgc tggcctatgg
    67561 gtggggctgc cgtcaatgtg gcgcagtcat cgattcgctg atcgatattc accgccaatg
    67621 gcatctggag ccccgcgcga cgtgggcgca tttggctgtc gctgcctgct aggtcacgca
    67681 atcactttcc ttgttgtcgt tggttcgcat gaccggttat ggcgcccgat gagccgaatt
    67741 cccggtagat atcaggaaga gtaaggagtg tgtcatgttg catgccgcgt tgattgctct
    67801 cgtgcttgga ctcgtggttt gggggcttaa ttggaccggg ctcgccgaga tgccgaccca
    67861 ggtctattgg gtctttacag tggggggaat gctgttgatg ctgatccacg ttgcgacaag
    67921 gcgaccggcc cgcgtgtggt aaggggaccc atcactatgc gactccgcta tgcatgcatc
    67981 ataagcgccg tgctgggaat tagtgcgccc tgcttcagtg aggtcgaagc tgcgaaggag
    68041 tgggcgccgc aggaggttac ggcaattctc gcgcagtcgt atgggcagct gctcatgcga
    68101 ccgatgggga tggagccaag ttcgcttcag aagaacgagg cggaccaaca acggtatatg
    68161 gtgcagtccg gctatgcgtt gcagttggca aaaggggaga aaagtgtgaa gcagattgtt
    68221 gaggcgatcg ccctatcatc ggagtttaac gccaagtgga taacgcccca catgggaact
    68281 gcgcagaccg gatcggcccc gggagtaccg cccgtggcgg ccccggaaaa ggccatcgat
    68341 agcctgtact gtgcgttgtt aggccggcgt gtagatagtg tgtcgttggc cagcgctcgc
    68401 aaggatttgg tgagttccgg ctttgaacgg gtaatccgtg acgtgatcga cgggaaggaa
    68461 taccgagacc ggttcggtga cgccaaagtg cctcaggtga gcagtgagaa gcaggcggca
    68521 gtcggttgcc cgcaagtggc tctgtaacag cggtttcgcg cagcaggctc aggattcact
    68581 agcgcaggtg gcttgacggg acggcaataa cctcagtgaa ggtgggggag aagcacggat
    68641 gagtatgacg ccggaagaac gcatcgcaat tatgacaaag gcgatcaatc aagccatcaa
    68701 ggtgatgggg gatcgtttcg ggtcgacaga ggaagatgtg gcgcgagcta tccaaggcct
    68761 gaaggcctca atgcagacgg caccgcagga gaagccagtg ccagttgggg ccgccactca
    68821 ggcctaagag ctgcggtgaa gcctcaccgg gcccactgtc tgatcggtga gggagccatg
    68881 tggttcggcg atgctccgat gtctctcgtc gttgggagca taggtttcat gtgtactccc
    68941 ttgctgtctt ccgacacgag tcaacctacg gactaggcct caatatcgct tgactccgat
    69001 cgccatagcg cttagcgtga aggtatgacg tctttagctc cgccgactgt tctctccctc
    69061 gcgcttcgag cctccatccc gcgcgactga tcgtccggtc tccgccgctt cttacgccag
    69121 agtgccctgc actgaccgat gatgccgtct tcgtgaacgg agcgattgtc gcttccggcg
    69181 aggagcggtg tcgtgaccag cagttccatc cgacaccatt atccgtttca tatagcagta
    69241 caagggagca ccattatgat taccatcacg gcaacggctg aagagaaaat tcgcgagttg
    69301 atgctggaag aaaaagatac gctcgggctg cgtgtatatg taaaaggcgg gggctgccac
    69361 ggctatcaat atggaatggc gttcgagtcg aagatgagcg acgacgatac ggtgattgaa
    69421 aagggtgatg tgaaagtgat catggattcg caaagcgctc cgctgttgag cggctgcgaa
    69481 gtcgattatg ttgacagcgt gcagggctcg ggctttgcga tcaagaatcc acaagccaag
    69541 acgacctgcg gatgcggtag ctcattcagc gcctaatccc ggcgcgtgtc gctgtctttg
    69601 catgatcgtc actgtgactg aaggaggtcc gccatggaac ccacatccgt cattggaaca
    69661 aagaataaaa aaggccaggt cttcaccgac ccccgcatca tgatgcggga ggcgcctcgt
    69721 acgccatcgt tttataccgg cagcgaagtc attaaggaag ccatccggcg cgccagtatc
    69781 gacgtgatga tcgcctatcc gatcacgcca caaagcgagg ccgctgcgtt gatcggcgag
    69841 ctgtttgccg aggggtatat cggagactat ttccgcgggg aaagtgaatt tgccgtcatg
    69901 tcgcaatgtg caggggccgc cttcggtggg gctcgtgtct tcacgacgac ggccggtccc
    69961 ggaaccatgc gggcgatgga aaacttcccc atgtgggcgg gtgcccgctt gccgatccaa
    70021 tgcatcgtaa cctgtcgcgg gatcaattcc ccgctgtcga ttcaaccgga caccatcgag
    70081 atctcttatc tgatgcagac aggcatgctc gtctggcatg cggagacggc acaggacttc
    70141 tatgattgga ttcttatggg ctatatggtg tcggaggagc ccgaggtgca tttaccattg
    70201 gcgctctgtt gcgatgggtt ctttgtgacg cataccaaag acgtcgtcga tctgaccccg
    70261 acagatatgt gcctgccgcc ctatgacccg tatcgctcgc cggtgccctg catggatatg
    70321 gaatgtccgc cggtcaggat gatgcgtgat ccgttcatca tgaaaagcaa ctacatcagt
    70381 tatgccacgc acgcgagctg gcagcaggaa gtctgggctg ccgcagaacg gtcgcggaag
    70441 cacacgatcg cctggttgaa cgggctgatc gaaaccgaag acgttgacgc cgatgtgctc
    70501 attgtcacat ccggtaccgc ggtatctcag gggcgggaag ccattcgtct cctggcggat
    70561 gaaggtattc gcgtgggact cgtcaagatc aaaacgctcc ggccctggcc ggaagaggaa
    70621 attaaggaag ccaccaagca cgccacgcac atcattgttc cggaattcaa tgtcatcggc
    70681 tggatggcga aagagatcaa ggccaccatt ccaagaagtg agcgtgtcat cgccggtccg
    70741 cgagtgtgtg gcgggatgac catgccgccg gagatcatcg tggaagagat taagcgtgcg
    70801 cttggcgtgc gttctggcac gctcgccggt cgcgggtgaa gcgcagaggg ccagacaacg
    70861 agaacggttt cgatacggtg tgcagcacat tgacgctcag cagggctgag aggagataga
    70921 tatgagcaaa gaacgcatca agatttcgcc ggatctgtat gacatcatgc cgtcggatta
    70981 ccaggacctg gtcagcagcg ccacgtacgg caaggaagat cggggatgga aggacatcgg
    71041 cacctccaag gaattgatcg agcagcattc actgtgtgcc ggatgtcctg aatccatggc
    71101 gtttcgatat attttggcgt cgcttcccaa ccccgaagac acggtcatgg tcggctcgac
    71161 cggctgtacg agtcttgtgt ttccgatggt ggccgtccac aacattcatt ccctgttcgg
    71221 caaccagaat gccatcgcgt cggggctcaa gcgtgcgttg agcgtccgct ttcctgatcg
    71281 tattaaggat gtggtggtgc tggcgggaga tggcgcgacg gtggacatcg gtctggacat
    71341 gacgctgcag gcctggttcc ggcaggaaaa attcacgacg atctgcttcg acaacgaact
    71401 ctacgccaat accggcgggc aggagagcgg tctcatgcag aaggggttcg ttgccaaaat
    71461 ggcgcccgtc ggaaaacttt tcgataaagt acgtttgcct gaaattgccc gggagtccgg
    71521 ctgtcactac gtcgtcaact gtacggtgag caaaccgtcg ctggttgaaa aggtcgtccg
    71581 caacgcggtt ctgatcgcac gggaaatcgg cccgacctat cttcagctct atacgccctg
    71641 cattctggaa atcggcaaga acagcatgga gggtctgcag gaaatgcggg actccgagaa
    71701 gcctactgaa cggtttgcct tcaaggaata tatcagcgag ccggcgaaac agcttctggc
    71761 ggagcgtgat gccaaggcca aagaaaagaa agccgctgca aagcaactgg tttcgtaagc
    71821 ggtaggattg tcaccgccgc ttccgcggct atgagagagc gaggtttcag atgagtcttg
    71881 attatgtaaa gttcacaccc ggttttgaac agttcatgcc gaaagaatat cgggatatgg
    71941 tcgagcatgg accattcgga aaaaagacga cggtgtcgca gatgggcagc ttcaaggaaa
    72001 tcctggagga gcacccgatg tgtgccggtt gcgccatgac cctgttcatt cggttggcga
    72061 tcattgcttt tccgaatccc gaagacacca ttaccgtcgg gacggcgggt tgtggaagat
    72121 tggccatttc gcaagcggcg attccgttcg tgtacgggaa ttacggcgac cagaacggtg
    72181 tcgccagcgg tttatcgcgg ggactccggc tccgtttcgg cgacaagccg aaggatgtgg
    72241 tcgtaatcgc gggtgatgga ggaaccgctg acatcgggtt tcagcaggtg ctgcattcct
    72301 ggtttcggaa ggaacggttc acgaccatca tgctggacaa cgaagtgtac ggcaacaccg
    72361 gcggtcagga aagcgggatg acgaccagag gcgccgtgct gaaaatggct ccgctcggaa
    72421 agaagttcga gaagatggat atggtcacga tggccaaagt cgccggctgt gcctatatcg
    72481 ccacggttgt gccgaacaat ccgcgacggg tggaaagcgt catcaagaag gccgtgctga
    72541 tcgccaggga catcggcccg acctatattc aggcgtatac ctcatgcaac atcgagtatg
    72601 cgatcccgac cgacaaggtc atggaagatg ccaagaccgt tgaggacgat cgttacaaat
    72661 ttaccgagta tgtcagcgac gaagcgaaag cgtatcttgc cgagcgctat ggctacaagg
    72721 aattcatgcc gaaggccgtg cctgcttccg tcacgacggc ctgaggagtc gtaaaggata
    72781 agcagttatt ctccagactc gctctggcgg gcaggagctg aacctcctgc ccgcccagtt
    72841 cgctaggcat ggggcgcagt tgactgcagt tgattctgat ccatcacact gccccacacg
    72901 agcggttctc tcgagtcttg ccagaaaata attaagccgc gtggaatcaa gggcttgcct
    72961 catcgacctt cgcagcctcc attgacacag ccggtcgtgc actgtagggt gtggcatgac
    73021 gtcttgagcc acgccgactg tcccatcttg cgcgcctcct tccatccttc gcgcgtctga
    73081 ttccccggtc ttcgccgctt ctacgccaga gtttccatct cagaccacca ggtctcggca
    73141 tgcgtttgcg cgtgtcagcc ctcacagcgg tgtggcgctg tgctcgccac cgataaagga
    73201 gaatgcaatg acacaggaac agttccaaca gttctggctg cagctcaaag ctcccctcaa
    73261 ggccaactgg gacaagatca ctgaaagcga tctcggcgaa atccagggca gtctggtgaa
    73321 gttcggtgat gtgcttcaga agcgttatgg tgaggggcac aaagatgaag tcagcctctg
    73381 ggctgaccgc cggcatgcgc attggagcgg aaattacatc ggctataagg atccaaagcc
    73441 ggccgtctaa ttctaattcg gtacctccgt tcagggagtc gatttctatg ggtgggtcgc
    73501 gccctcgctt gcgataagaa acagaaagag agtacgtgtt atgcgaaaaa tcgtcattaa
    73561 taagagttac gatcagttct gcctgagcca caaagcgttc ttgcgtctgc gtgagttggg
    73621 tcagccggat gcgctcaagg agacggatct gggagcctac tggccctcag cggccggccc
    73681 tcaggagccg agcctcaacc aatgcggcat gttgattcca cgcgacgaca agcgcttggt
    73741 tcaggtggtc gaagaattgc agggtgaggc caatggccat tgtgcccaac tgaaggtcgt
    73801 gtcgatcccg gacgacgtgt catgggtcat cgcgaaagcg aatggcagtg agcatgtgag
    73861 cgaacagcat cggacgtgga gctgagccgg tgaggaacgt agcgacgggt acgcccgcct
    73921 acgggttgga ttgaactagg gtgcctgata ttacggggaa gcgaggcttg tctgcccgtg
    73981 cgagactctc aaagcgtacg gtgacgagtt tgacctgcct ggaaggggag gttgaagacc
    74041 tgctttcgga cgattaatct acccagatct agcctgcctt ctctcaaatc gcgagcagag
    74101 tctgtctctt tccacggtgg cgactctgct catagcatgc tgttccggtc ctgccttttg
    74161 agcccaagcc agagccgagt tggttggtca ccccggcggt agaaacacgg tcgttgtgag
    74221 cgagtctccg gccgggatca cgattgtgtt aatacgtcca ggtcagcagg gtggtgaata
    74281 cgtttttgtc ataggcatag acggggacat tcgaaccgtt gagcacgcct tgatattgaa
    74341 gcgcgaccgt cacgttacgg aatagcggcc tggtgatttg aacgaaaata tcctgttcgg
    74401 tatcccggcg ccgggacaac tggccggcgt cgtcgagaaa aatgacttgc gcatggttgt
    74461 atgcgcgcca gtgcacttca tagtcgactc gcaaactgag atcataccag ggaagagtgg
    74521 tttgtccgcc cagctggagc cgattgccgg aataggcgaa gctggatccg ctggcagctt
    74581 cggtatcgtg ctggtagccg acacggacaa tgatggtgtc ctgtcgaaac cggaaggcat
    74641 ggagaacgcc gatcatattg ttgaaggcat cgcgcgactc aggagcgaac cggatgtcgc
    74701 tgttgccagg ctccgtgaag aagcttttgc gctgaaagcg atagagcagg gtcgtcaagt
    74761 tgtccatgcg gccgagggct ggaagcgtga tcgcgggcgg aacgaaagtc gcagggaggg
    74821 tcacggtgtg gcgggagaga aatcccttga gaccgaggag catgtaatcg tagctgtact
    74881 gaccaccgat ctcgtagggc atcgtgccga cggtgccgcg atagacgcct gacaagcctc
    74941 cgaggtgatc ttgaatgttg aagcttcccg cgccgctatc ggtatttacg gtttggtaat
    75001 aggaataggt gacggttgat tcgatcggtc ctttgcgata ccaggcataa tctccccgcg
    75061 ccgacgtgac aaacccgggt gattgggtgt tacgggagcg aagcagttct gcaacctggt
    75121 cgctgctctt ccgtggattg acggcgacat tgtcgtcata atagcctccg accgccaatt
    75181 gcatgcgaag ccgttgaggg tccgtgactc gtgttgttgc cgtgagggct tcttgaatgc
    75241 gtacagatgc gccggtgatc ggagaagtgg gttgcatccg ctgcgccgac gccaattcgg
    75301 cctgcgcctg atctgagagt ccgagtatcc ccagcgcgag gcctcgatag aacatggtca
    75361 attgccggat gtcttgatct gcggagcggc tgacgctcaa tgccgcctcc gcgccgttat
    75421 agttcttctg ccgatagcgt aaaaatccca cataaaatcc gaggttctca cgatccggct
    75481 ccaatcgaaa cacgtcctcc aaaaggggcg cggccttgtc atagtgtttc gtggcgaaat
    75541 gggcgacgcc caattggtag gtggcttctg ggtcggttgg gcgaagggtg tgcaaggcag
    75601 tgagaggcgc gattgctagt tccgcctgtc cccgcgccag gtgacagagc gccagatagt
    75661 agaacgctcg cgcattgcgc ggatcggtcg cgactgctcg agagaagagg gcggcagctt
    75721 cgtcataacg tttggcctcg taggccaaga ttccttcggc aaccaacacc tccgcatcgg
    75781 cttcttgagc ctggacggag ccaggtggca cggccattgc catcaggatg aggatcatca
    75841 taagaagaaa gctgaactgc gggtgcgtgc cgttcgaagt cctgcctctc tgctgtgtcg
    75901 tgcatatcat tgagttggtt ccccgttccg ggctccgtaa tttctcgtgc atacctgccg
    75961 cttgacctgc gcgtagcgag ccacctacta tacgccactg tccgcctgtt ttccgagtca
    76021 gcatggccgc aaagtggctg ctgtaaacga tttgcaagat atgcgttgaa ccgtgagtgt
    76081 tcgttttaac ccgttcttga acagggtgat tccagggagt gtggtccaat gagaatcttc
    76141 gatatacgct cgcaagttct cggctacagc gtgatgcttc tcttgagtat gtcaacgatg
    76201 gcatgggcgg aggagccgcc gctgcctctg tccggtgaag gtcaggcgag aggggtcgtg
    76261 cttgggatcg tcggaatgcc ggtgaggatc gttgctcctg ccgccacccc tggtggaact
    76321 ttgaacgtcg gtgacgtcat atccgcgggg gatgagttgc gggttggaac gggagagcaa
    76381 gtggccctgt tgtgggatca tcgtgcagtg ctgacgttgc gtgaaggcgc gcgaatgaac
    76441 attcaggaat cccaccgtgg acaaacggaa ctcttgctgc accaaggaat ggtccggatc
    76501 gcattgtcct ataacgcggg gagaatgacc gatactctga cgctccagac cccgctggca
    76561 cgcgtcgtca gccgtggcgg cattatggag gcgacggtat tggggggaga gcggcaatca
    76621 cttttggcgc ggttgatcac cgcgcctcca ggcgatacgt tccgggtgtt ggaagggcag
    76681 gctcgtgttg agccattgac cggagaccgc aaggcatttt cccttaaagt agggacggag
    76741 ttcgcattgc gaaccgggat ggtgacttct ctttccgagc tccaagaggg ctctcacgtt
    76801 ccgcagcctc ttgccgtcag agaggagcat cgagcccttc ctagtcctgt cacgcggcaa
    76861 atcgtcggaa cacaggtcgg gcacgcactt gaactggaaa aagagattcg gcaggcttct
    76921 tctgctggag gggagaagga gcttcccggt ccctcgacca agggaactat cctcgcaacc
    76981 agtatcgggc tcccggtcgt cacttttagt caaacgccaa gccaagtgtc tgtaagtgga
    77041 acctccaccg gtgtgccgac cgtcttgcct ccgccggcag tgcctcccgt tcaaagcgcc
    77101 ggtgtcggga gtttagggcc tgcgcaatcc ggaggtttga acagcagcag gttgcttcag
    77161 gaaatcttga ccgaggtcgg aaagatcgga aagggccatg ggaagaaaga tcggtaggaa
    77221 cgtgcagcga gcacgagtta ccctttgaat gagagacagg ccggagagcg aactctcatg
    77281 actgcatctt gctgaaaggt atgctggtac gtgatcatga tgcgttggat cgcggcttct
    77341 ttggccatat tctgttcgtg agtcagctgc agaatgcgtg aggtctcgta ttcgatcgcg
    77401 ccggtgctct ttcgccactg accggacgcc tgccatgagg tgaggccttc cggaaatgcc
    77461 ggtgtgacaa tgtccttgac gaaggccgtc cattcctcag gactgacgat tcccccgggc
    77521 ttggccgtcc cgaaatacag cgactcaagc accgcaggat gctcgcccgg taaacaggcc
    77581 ggtgcccgag cagagaggca gccagcggtc tgtatcagtt cggacagtag aagcacgata
    77641 gggatcaacc gtctcatttc agaagcctgg tgtgtcagcg ctacttctta tccttggtcg
    77701 tgttctgaac ggaaggcttg ggtggagatt tttctttgcc gccgcccgtt actttcttga
    77761 aggtttcacc gatcgccggt ccgattttcg gaatttcttc ttcgatgttt ttcgccgcac
    77821 tcttcaagcc tcgcccgata tcgtcgaggg tcaggttctg gtcggatccg ctctttgccg
    77881 tgccggagtt ttccgccgcc gcatgcgagg acagagtaaa cagaaagcta ctcagggaca
    77941 gagcgaatgc aatcagtgtc gtacgcaccg cgggcctccc gttgttgacc gtagtataag
    78001 gcatttcggc cttgcggcaa gccccgtctt ggtgccggta ggttgccgga actcgcaggc
    78061 cagttgaccg gtaggggcac catgcggtag agtgaggatc tactgcagca ttgtatcggt
    78121 ccaccaatgc tgtacctcct caacgtggac cgcgcgatcg catcgcgacc aggtccagtt
    78181 tggagctgac tccttccgga ttcaccctcc cctcggttct ctgatcccgt cttaatgcga
    78241 tccacaacga aaggatagct gcgtgcagac gactgcgttt acgagttttg actcccttgg
    78301 tgtgtctccc accctgttgc gaaatctgac taaggcaggt tttgctgaac cgaccgccat
    78361 ccaggctcaa gccattcccc atgcgttggc cggccgggat gtcttgggat gcgcgcagac
    78421 cggaacggga aagactgcgg cctttgtgat tccgatgttg gagcgtctca gcgggacccc
    78481 gaaagggcag cctcgcgcgc tcattttagc gccgacacgc gagttggcaa tacaaattca
    78541 agcgacgatc gatacgttgg gtcgcgatct gcaattgttt gccacgacgg tggtgggcgg
    78601 agccgatatg caggctcagg tgagaggcct gcggcagcgt cccgatatta tcgtggcgac
    78661 tccggggcgg ttgctcgatc atatgtggaa cggcacgatc agcctgcttg ccatgtccat
    78721 tctggtcctt gacgaggccg atcggatgtt ggatatggga tttgcccagc agatcaatca
    78781 gattctggat gccatgccgg aagagcggca gacgttgctg ttttccgcga cgatgccgaa
    78841 cgatctcgcc agattggcgc aggccagcgt gaaagacccg gtacgggtca tggtcacgaa
    78901 atcggcgacg acagccgacg gtgtgactca agccgtgcac cacaccacgc acgatcgtaa
    78961 gaacgggctg ttgatgtcgt tgctccagtc cgagtccgat accgtgctgg tgtttgccag
    79021 gacgaaacat cgtgcggatc gattggggaa tctgctcgat tcggccggtc accgcgtcgc
    79081 ggtgctccat ggcggaagga ccctgccgca acgccgcgcg gcgttggaag ggttccgccg
    79141 aggtacctat cgagtgttgg tggccacgga tattgccgcg cgtggcattg atgtggcaaa
    79201 tatcgcccac gtgatcaatt acgacgtgcc gaattgtccc gaagactatg tgcaccggat
    79261 cgggcggaca gcccggatga ggacgaccgg tcgcgctacc acgtttgtga cgtccgagga
    79321 ccaggaacaa ttgcgggcga tcgagcgttt actcgggcag gcggtgccgc gcgccgaggg
    79381 gagcccggcc tcgatgagcg cctcgcctcg accggacggc catgcgccac ggagcgaacc
    79441 ggagcgtcga cgccgacgcg gcggatcttc tcaggtgtgg agtcagagcg ccgacggagg
    79501 tccccgggag gccaatgggg gaatcaagaa cggaagtgat cttgggccaa agtcttaatt
    79561 tttccgcgca tacgtagcaa gggaggaatg aaggtgagcg atcagaatca agatgcgcct
    79621 cgggccgcca gtcagtgggt cagaattcct gatggaacga tggtacagca tcgattggat
    79681 gggcaaaagg ggcatatcga cggattgact gaaattgtga acggcccgag tcggaatccc
    79741 gatggtcgga ctcagtatcg aattaacgtt ggaaagggag atcgggtgtt gattgccgag
    79801 gaagatctct tgatcctgac ggatgccgag gggttagtgc gaatggccaa ggagaagggc
    79861 gaatatcgct cgctggtcag caagcaactc cgcggtgtgt tcggtgaaga ccgctttgta
    79921 attaaggcat cctaagccgg ccgagtgttc cgggagtacc caggagcccc tgttcagtac
    79981 aggggttcct gggggcccca ctgcaaccct tccaggtcag gatcatctcc ccccgatgtc
    80041 accgtgcgct cgggtttttc ggctttgcgc tggactcgac gggcttcttt ttcgcgctgc
    80101 ttcacttgct tcgctttttc tcgatcgcgt ttcgcgatgg tggttttgcc tgctcgtgcg
    80161 atggttccct ccttctattc gtcttccctg atcggtgatc gaataggcct gcctggttat
    80221 gatccggacg gtcgacgagt ggtgcgagtg gcactggtac gggcatgggc acgcgcaggg
    80281 cgtgcctggg tctgttctga gcgggcacgg gtctccgcac cgaaccctga actggccaag
    80341 ttcgtgaccg cacgacggat ttcatccatc ggcgtctccg gcgagtccac agagggatcg
    80401 acgagtccga gctgtgtcca acggattctg agcttcggga ccgtgcgctt tttcttatga
    80461 ttgggtttgt tgccgcgcca gacggacgta attttcatcg tacactcctt tcggcgaaca
    80521 acaggatggc gatgtccagg ccatcaaact ggttgcagtg ggtcgggcat gcggttcgga
    80581 ccactgcttt gcccatttcc ttgtccgctg aatgggggaa atgggccgta ggaggagcgt
    80641 aagttaagga agcgatcgcg ccgtgatagg ggcggaaacg cgaagcgtgg gatttcgctc
    80701 ggggctcgta cggttcagca atttatcatg ctttccatag tccggtcaaa tgagctgatt
    80761 ggcggaagca tgacctggct tcggctggag cgcgcgttgt catactgcgt cgtagagggg
    80821 gaaatcgaca agggcaggtt ccaccgtcgg tccagcaatc tccatgggtt aggggagagc
    80881 cgtaggttcc ggcgatatgc cagccggccg tcgcagccca agttttctca ggcggctccg
    80941 cagcgtttcg gggttgagtc ccagcagatg cgcggccccc tgctcgccat agattcgcca
    81001 tttcgtccgt tccagcacct cgatgatgtg gtggcgctca agatccccca agctgactgg
    81061 ctggctgctg gaggggctgt gaacggcctg cacgaccggg agcaacatct cgtctacggt
    81121 gacttgggag gagccggaaa gaataacggc gcgttcgatc acattttgaa gctcacgcac
    81181 gttgccgggc cagttgtagg tgaggagccg cgccatcgat tgtggatcaa aggcgagatg
    81241 tgcccgcttg agcttgcccc cgatctgcgc caggaaatgc tgcgcaagca ggtgaatgtc
    81301 ttcccgccgc tcccgtaagg gcggcagggc gatggggaaa atatgaaggc ggtagaacag
    81361 gtctgcgcga aacgtcccct gtcgaacggc ggcctggagg tcggcgttgg tggcagcgac
    81421 cagtcgaacg tccactgcga cgggttgggt tcctccgata cgatcgacca taccgtcttg
    81481 caacactcgg agcaacttcg cttgggtttc caacggcatt tcaccgatct cgtccaggaa
    81541 caaggtgccg gtgtgggcca gttcaaatcg cccggctctt cgtagctgcg cgccggtgaa
    81601 tgcgcccctc tcgtggccaa acaactcact ttcaatgagt cctgatggca gggctgcaca
    81661 gttgacgcga ataaagggct tgtggctgcg gaggctcaaa tcgtgaagag cctgggccag
    81721 gacttctttg ccagtgccgg tttctccgag gaggagaacc gtcgtgtctg tcggggcgac
    81781 agccttcaaa agatcgacca cctttctgaa ggacaaagag gttcccacaa ccaggcgcag
    81841 attgcggctg gctttgacct cttctgcgag atactcattt tctcgacgca agcggtcact
    81901 cagctgctgg atctgctcgt acgccaatac gtggtcgatc gcgtatgcga tctgggtggc
    81961 gacttgctgg agaaatttac aatcgtcctg atcgagatgc ccggtctgaa cgctgccgat
    82021 gttcaacgtg ccgaggcaat gttcccggac gagcaagggc agattaatca ttcgtcccaa
    82081 accttcctgc acgtaatacc gatcctccag aaagacttgt tctttctgga gatcctctct
    82141 gatatgcagg cgacggttat catggaccca accgactgcg ctgcccttgc gtggaatgac
    82201 gctatcgtgc gccagggccg gagtcgccat gttggtgatg acggcataaa agcgaaagga
    82261 atcggtctgt gattcgtaga gcgtaatccc ggccctatcc caagggatca cttttcgaat
    82321 ttgatcggcg atgacgcgcc agagactgtc gatgtcccgt tgcgagttca gggcgttggt
    82381 tacttccagc agcgtttcaa aattcagtgt ggcgcgttgc atagtcgtct aaaaattcgt
    82441 ccagagttgc acaatgcccc agtcctggtc cgcggcggta ccgagctggt gcttgatata
    82501 ggagcccgaa aagaagtggc cgtagatgat tgacagtgag accgtaccgt ccgcaaacat
    82561 atggctccat gcgatgtcga gttcatcgcc gatgtgagtc gcccggttgt cggacgcgga
    82621 gaaaatcaac ggcccttgtg atccacggta ccaattgtct cgggcgcttg ccaagtactt
    82681 gcgcagcgcc cagagttcga tatggtctcg agcggtcggt cgagcctgga cattgacctg
    82741 cggctggacg ctattgcgcc aggcgccgtt gagcatgtag ccgacgtgca gaaaattggt
    82801 cgggaaaaaa ttctcgaatg tgttggcatt gccgccgcaa ctgcgtgcca ggtttccgcc
    82861 gggggggaca cagttgctgt cgccgtctcc ggaggcgtag tcgaatccga tggcgacccg
    82921 gggcttccaa tgatgggtat accatgtgta gccgagccac gtcccggaag cccaggcatt
    82981 gatacgcaga ttgcgccggt tgtcgaaatc gaatccatcg gcgctacggc cgaattgata
    83041 ggccgcttca tgggtgaaat cccaattgcc gtcccggaac tcaatgcgga ggccgaccat
    83101 gtgccgtaac tgagaggccg atttgggtaa gtattggccg ggattggccc gctccggaag
    83161 acggtttgag tacaggatat agtagggctc tacgatcatg cgggggatgc tgcggagttg
    83221 actgtagaac accaccagat ccacgtcgct gcccgcgtcg gtcgcatgtt gcgcaagggc
    83281 cggattgcag gtggccgagg agtttgcctc cgggttgcat gtcaacaggt ttggtgtaag
    83341 gcttccaccc gggctcgcct gccccaagtc cgtttcggaa tttcgaaacc agcccagttt
    83401 cgtatcgaag gcggcggtcg cataactcag catgatgccg tcgtgcgaat agccggtatt
    83461 ggcccagtcg aaatggccga agagtcgttg gttgccgaag acgacatact gccggccgat
    83521 cttggcgctg aacccatcga taccggcgat gttcctgacc agcatatagc cggcgcgaat
    83581 gcctaagcgg cattgtcccg ctagctggat cccacaatta tgagtgctcg cgtcacccac
    83641 ttgattcctt ccggtggggt cgccattccc cccccatgtg gcggagtctt gtagctcgac
    83701 ataaaaattt acattggggg aaggatcata gcccaggcca atgcgtgccc actgctggat
    83761 gaagaaatcg ccggcatgac gaccgctatg agctcctgtg ccggacccga attgatcgag
    83821 actgttgcag gcgccggcga tcggggggcc gcctccaaaa cagacgctgt tgcgccattc
    83881 aggtctgacc cggagatccg cgcgcatcca gacatgctgg agatcgaagg acgacccgag
    83941 gggcgacgac accgcgtcat ccggttcaaa ccgaaggcgc acgggtgacg atgacaagcc
    84001 tggtgaggca ggcccatatc cggcgcgttc gctttctgcc cacaccggag gcaagacaag
    84061 ggttgcgcct gtgctctcaa gcaggatgac acacaccagc aggacggttc tcgtgagcgt
    84121 agatcgtaac atcgaagatc atggaacatc ggaatcctcc attcactggt gcggggcggc
    84181 gggccgacgg cctcgggtgg atggtcggcc cgccgcagat tccacagttc gatgaagcca
    84241 atggggctta gaaagccatt gacgctcttt cttccatgtc ccgcataaag tcttcgtgtt
    84301 tctttacttc atcggccccc tttgcccgga tatggcgatc gcgtgcatgg tattcatccg
    84361 gggtaacgcg atagcagaag tcccacaagg cgtcggcgct gtgcgcatcg attcctccga
    84421 ccctggcttc cagcatgacc atgagttggt tcacgccgtc gtaggtggct cgccggtttc
    84481 ccgtgcggta cagttcgcgc actttacgca tctgctccac ataggcctca aaatttcctg
    84541 ggatcaggct cttctcggcg agtgtttggt aggtcacaac ctggcccagc agttcttccg
    84601 cccaggcgga agatttttga ctgttgctcc aggtcaccgg ctcaatgttg tcggcggctg
    84661 cctgggtggg agcggggccg agtaaggccc agctgcaggc cagcacgatt gcggaatagg
    84721 ttgcgatgtg tcgcttcatg ttgtcctcct ccttgaggtc tatccggcag aaatttgccg
    84781 ggtggatggt gtgcatggtt agtgagccac gatttgctcg taggtagagc aatacctgtg
    84841 cctttacggg cgaatagggc gaagattgcc aacgcattga attagtgcga aaagttgatg
    84901 caggcgctca tgtggtgcgg agtacaccgt ttctattcac ggtgacggcg tgaaataaaa
    84961 cggtgctgat cgttggcccg atgagttgta acgggcaaaa caatggtgcc gatcgattgt
    85021 ctgcgatgtg ggcgtctacg aggcaagtcg atcggcatgc ggcaggtcgc cgccgagagc
    85081 ggagatgcgg aaagttacaa agggtttcgc aactgcggag gttttcggta gggtaggcca
    85141 agtgtaaaat ccggcggtat tgaaggggag caagcgcccg ccccgatgcc ggcgaacatg
    85201 gcggggagca gtttcggaat aaggcgcgta ggaggttcct cgtgaggcgt gggaggcgaa
    85261 cggaggccta tggataaggc gctgtatccc tatctgctgt tctggagtat cgggctcctg
    85321 tgttttctcg ccgagtggcg atttcctgca cgtccgattg cctaccggtc tgtattctgg
    85381 cgggatctga tcgcgttagg tttgtacaat gtatcgttcc ttgccgtggt acaggcgacg
    85441 gatcggattc cgatcccgaa ctatatgcct gcggtcctgt acaatctgcc cacggtcgtg
    85501 aagttggccc tcttctatat cgtcgaagat ttcgggctct attgggtgca tcggctcatg
    85561 cataccaagc cggtgtggcg catccaccgt tggcaccatt cccccacctc cctgtactgg
    85621 ctttctggaa tccgcgccac cattccgcat atcgctctgt tcaacctgac gtatatcatt
    85681 gctcttccgt tactgcatga ggcttcggca tgggcatttc aggtgatcat ggtagagcac
    85741 attgtgcgga acaattggat gcatctgaat gtcacctgga ggtcgtcctg gctggaatgg
    85801 gtgtttgtga ccccgcgata ccatcagatc catcacagca cagatcctgc tcatcaacgg
    85861 acaaatctag gcgcgttgct cacgatttgg gaccggctgt tcggaaccta ctacaatccg
    85921 gacgatgtca gaggtgaatt gtcgtttggc cttgccgaac gggtcccgcc ggtgaggctg
    85981 gtcatgggat tgtaagagta ccattgcgtc gcaagcgttt cgctgtcttc ttcgagctgg
    86041 ttctcggcct gatggacaaa gcctggcgtc gatcacggtc cgggccggca ggtgtctctg
    86101 atgcttcgcg aaagccattg agagggtact agtgcaaggg cctgtcctgt ccatcatcgt
    86161 gccgacattt aacgaacggg acaatatccc ccggcttgtg gcggctctgg acgcctgcct
    86221 ggttggggta gagtgggaac ttatcgtggt cgacgacgac tccccggacc agactgccgc
    86281 agtggtgcgc gcgcaggcct gcgccaacca tcgggtgcgt tgtctccatc ggattggacg
    86341 tcgggggttg tcctcagctt gtatcgaagg gatgttgtcg agttccgcac cggttgtggc
    86401 cgtgatggac ggcgaccttc agcacgacga acgattgctc cccgacatgc tcaaggtctt
    86461 attgcagggt gggtacgaca tcgtggtcgg gagccggtac atggcgggtg gcagcatcgg
    86521 cgagtgggat ggcctgcggg ccttgaccag tcgatgggct acgcggttga gccgcccact
    86581 ggtgcctgcc ggattgacgg acccgatgag cggctttttt gccatgcgca gagaggtgtt
    86641 cgagcggctg gtgagacgga cgtccgggat gggttttaaa ctcctgctgg atgtgttggc
    86701 ctcgtccccg catccgttgc ggttcataga actgccctat gaattcaggt cgagacaagt
    86761 cggggagagc aagctcgaca gtcaggtggc gtgggactac ctcatgctgc tgcttgataa
    86821 gtccatcggc cggtttgtgc ctgtgcgcct ggtgacattt tgcttcgtgg ggggctccgg
    86881 cgtcctgatc cacttcagtc tgttgtgggt gttgtatcgc tggttggcgg tgccctttgc
    86941 ctgggggcag ggtattgcca cgttgcttgc gatgacgagt aatttcatct tgaacaacat
    87001 tattacctat cgggaccagc ggctacgagg gtggcagtgg ttgcgagggt ggttgtcgtt
    87061 tgtcctggtg tgtagtcttg gcgctgtcgg aaatgtgggg gttgcgtcgt atctgttcac
    87121 gcagcaattt cattggggat tgtccgccct ggcaggcatt cttgtgggga cggtatggaa
    87181 ttatgccgtc acctcgtcct acacctggaa agtcaaggct cggtgagcaa tcgggattgc
    87241 cctgtcggcc gtgcgttagg gctgtaccct tccggtacga taaaactgga actcgtctcc
    87301 tgagccgaat ggaacgaagt cggcaggagg ggggggggcg caggcgttgg gtgcgtcgga
    87361 cgaccacgta gtcgtattgg gcgcggagct ggtcccaggg aatcggcgcc gcacgaaaga
    87421 ggaattcttc taccgtcagc agtgcatgga ctgccggcgt gagtcggacc ggttgctggc
    87481 ccggtgcggc gaagagattc gagacgaaga ccgaccgccg gatgactccc cagcaggaca
    87541 ggtttaccat gggcatgggc tcaggtatct ggccttctgc cggagaccag ttgtcatagg
    87601 aggccatttt aatggccgac agcaggcgcg ctccttccgg cagctgatca aggatctgtt
    87661 tgaactgtcg gtagtggtgt gccgtgttct gccagtgctc ggtgatgtag cccatcctca
    87721 ccgcgaatac cgtgctcagg gccagtgcca ccggaagaaa ccatcctcga ctgatttcgc
    87781 gccaatccga actcgccacg gtgagtaaga cgaagacgat cggcattcgt atatcgacca
    87841 ccgcggttgt gccgatcgta ttaggcatcg ccatggcggc cagcgcggtt gcgccgagcg
    87901 ggaggaacat cgacggcagg atggagcaac gtcgccgcac caaccctatg ccgaccagcc
    87961 cgaccagcat gaggacggtg acgcgatcta agagccggtg ttcggttcgg atggtccctt
    88021 tcagagcttc gagttttgtt acgggagaaa gaaaggagat gagtgggggc ggggaagatc
    88081 ctctgagcca caagggatag tcttcggggg ccactttgaa ggtcggggtc agtgtcagga
    88141 tgatgagtgg aatgatgagt gccggagcgg ccttccagca ggcggcgttg gtagggtgaa
    88201 tggcctcgtc tcgcaggcgc caccattgag agagctcata ggtcgagacg atcagcgcga
    88261 agctgccgaa tgcgaagagg tgagcgaata acaacatgac ggcggtgagt gaaaataacg
    88321 gcacgaccag gagcgcggaa cgagtgcgga gcctgaccca cagggcgcag gcgaacagag
    88381 ccagtccgag cccgaagaga tagttgagga agccccacag gaagacactg ttatagagaa
    88441 agaaaaatgc caggagtggc cagggagacc agcgtcgatg cagggcagcg tgaagcgcca
    88501 tcgtgccgcc cgccagcagt gcgaatgtca gcgcgatgaa catcttgcct gcctgctcaa
    88561 ccgacatgat ccgggtaagc ggcggcacca ccagatccat cgaaaggtta ggaagcaggt
    88621 cccagtggat gtcgtagtat tggcgaagcc agggggaatg cccgccatcg gctaggatgt
    88681 gcatgcgggc caggtgattg ggatagtcta cgagcggcgg gatctcaacc gtcatcaacg
    88741 ggaccagcgc gatgcacagt agggcgaagg cggcgccgag gatgccgaat gcagtgctct
    88801 tgtcaggtga tgagttcgcg gtggtcctcc ttgaaaaacc cgttgcctga cttgttgtaa
    88861 ccaagcgggc gccggaaggc aagaatccaa ccatcactgg tttgggagga ttgtcggaac
    88921 cggtgcgtgg ttatttagcg gcggtatcgc cgagactcac aaattgcttt cgaaacccga
    88981 cgaccttcgc gagatagtcg cgggtctcct ggtaggggag tccggtacgg aggcggtcat
    89041 agagtgcggc cggttgtaat ccattgatct gctgcaacgc gttccgctga tctttggaga
    89101 atgtcttgaa gacgttgccg gcgccggtgt tataggctga gatcacgcag tattcacgcg
    89161 agaccaggtc ggtgacatcg tccaattggg cgtacgtcag cacgttaaga taggccgtgc
    89221 ccaattcgat gttgttgtcc ggatcgaaga ggtagtcgcg cgagggcgcc ttgtcttcac
    89281 cttttgcctt gcggtacgca tcccgccctc cgctggtggg gaccaattgc atcaatccgt
    89341 aagccggcgc agaactgacg gcaaagggat tgaagttact ctcggtgcgg ataatggcaa
    89401 agacaaggct gggactgatt tggtagcgct ccgcaaactg tccgacgacc gcgcggtatt
    89461 tctcggcctg tttgtgcgag aaattcgtga ccatcgagat gttgaccatg tgggcggttt
    89521 tcggtccctc ttcttgctcg accttccggg tggtcgtgct cttggagagg agtgtgtcgg
    89581 cgaactgttc cgcttccgcg ggtgtgcgca ccggcttgcc ggcttgatcc agcacgagac
    89641 ccaaaaggta cggctctttg tcactggtca gagtgatttc tttgtccgta aacagatcga
    89701 cgctgcgagg atcgttggga gtgaggaggg tggtgacgac ggcattcttc aggctggccc
    89761 gcggatcctt ctcgtcaagc gtctcgacca ggatattgcc cgcatcgaaa tccacgatgg
    89821 cgcgactgcg gtaattctgt gtgtatttca catatttctt ctgctcgggc agtttcactt
    89881 ccttggtgcc ccactttttc ccgacttctc cggtcaacgc cgccatgagc gcttggaagt
    89941 cccgctggat cgagcgaagg tcccgcaaca gggcttgggg gtcccgtcca taattctcga
    90001 cgcgccgcct ggcgatttca gccggatcct tgccgccgac gatatccagg acggttcttc
    90061 ctgtgctgct gccgacagcc cgttcggcgg cgccgagcat tttgtcggtt gtttcgcagc
    90121 ctgaaaagag ggcagggatg gagagactca gcagcaggag gggcattcgt ggaagagaca
    90181 tggcgcgagt ataccgaagg tgtcacagag aggaaaataa aatctctcaa tggcgcgtcg
    90241 ctaccgttcg gcgcaatagc tggctccttc agtacctgat cctttggcgg ctgaaccaca
    90301 ggtatatcca cgtgcttgac gctcggtttt gacggccggt accataaacg atcattggca
    90361 gaagttattg gaaggcagaa cgtatgaacc acagatttgt cagcctgagc cattcttgtc
    90421 cctcgacttt gagatccgtc cggggcttga tcgtctgtct actcgctgtg atgttggtgg
    90481 cggtatccgg tcatgccacc tcttcgtggg cgggacagac tcctacggag gtcgtcgagc
    90541 gcacgatgaa ggacatgttg tatatcctga cggagttgaa ggatgcgagc cgctctgcgc
    90601 agcgtcagtg ggaaatcgag caggtggttc ggcgggtctt caactatgaa gaaatggccg
    90661 aacggtcgct gggcgagacc gggatcaagt cgaatgccgt agagcgtcgg cagtttgtgc
    90721 gactgtttgt gcaggtgtta cgcgatgatc tggcggacca cctgcgagac tattctgcgg
    90781 cccaagtggt ctatctcgct gaaggaaacg atgccgacgg ggcacaagtc atgattgcgc
    90841 ctgccggcca ggaagtcgac acacggatcg aatttcaagt ggttcggcga tccggggctt
    90901 ggcttgtcaa tgatatcagc atcgacgggg ccagcatcat ggcgaagtat caggcgcaat
    90961 tcacgcgcat ccttcgcgag ggttcctttt ccgatctcat ggaatatctc aaacagaaag
    91021 cagtcattgc ctaggtgctc gacagagcga tccctgagcc gttagccact gtctcccctg
    91081 gcctggagcc ggttcatatc agctggacga ggttcctcat ggcacggctc gttgctcttc
    91141 atcacgacga tgaatggggt ggacgagctt tccgacgcgc tcaggctgac tgttccgaac
    91201 ccgctacgag gtgtgcgcga gcggctgtcc cattcgcgtg gagtttgacc aggccgtatt
    91261 tgtgcgctcg aatcaggagt tgctggcggc tgtgcacgtc gagtttgccg aaaatgctgg
    91321 tgaggtgatg gcgcacggtc gtgctgcaaa tgctgaggcg gcaggcgata tctttgttgg
    91381 acaggccctc tccaatcaag gtgatgatgt catgctcacg tttcgtgagg tcctcggacc
    91441 aattcggcag aacggggatc cggtcctcga agccgcctgc tgagccggtt gtctccaacg
    91501 cgcttgtgtc gcaaggatga cagcgcgcca cgttcgccgg acgccgacag agatactcga
    91561 tgacggcaag cagcacgact cccggctggg tggtcaacac catgccgtct attccggacg
    91621 acaaggcttc cagcccggct cggctgtctt caattccatg cagggcgatg attcgtatgg
    91681 tcgaaagcga agccttcacc tttcgaacca gcccgaggat gtccatgccg ggctgcagct
    91741 caatgatgag cagctgtggc ttctcttgcg tgacgatgct caccgcctgc agcatgttcg
    91801 tcgcctctcc gatcagccgg atgtgcgcgt gcggtgcgat gacggcttgc agtccaagcc
    91861 gcaggagctg attctggctg acgatcgcga tggtaatgag ggcagagccc gatccgcgtt
    91921 cgtgaatcaa tcagtcctcg aatccgtagc cttagcgcgg tgagacgccg gaatgggtca
    91981 ccgcctgtcg tcggcaatac tcgtaacaac tggacaggac gaggtcacct gaagggcgaa
    92041 ccgtgccaca gacctgcccc atcaggagtg cccattcggc gacgtgggtt gccagtcgga
    92101 ccggttccag gccatgggtg ctgaacagat tcagcgcaac cgtcgacagc acgaccttga
    92161 ggtacgagag ggacggacca tagctgaaga catattcatg ctgcccggag aacaacagct
    92221 gcagcccttc tcgtgccctc atcccacgat gcccgcagtg tccacactgg aggcattcaa
    92281 gaccagtgcg tgcgtcatag cctgcacgat aatcagatgc cattagttgg ccgcagttgg
    92341 tggttgggca gagcatcgta ctgttctccg tgcaggatct tccgtgtgcg atcttctcat
    92401 cgtcccacgc gtgacgcctg gacagaggtc gggaggcggt cagatctgtc gtggcgcgcg
    92461 cgcgtcggtt ccgtcagtgt caatcgttcg gcttccagcc agtcttgacg gtcgtgcccg
    92521 tcctgccgcc ctcgttgctg atagagctca taggccaact tctcgatgcg cgtcctgatt
    92581 tcctcggtcg tcgcttcgtt gtcggcctgc tgcggcgggc tctcaggacc cgaccgttgt
    92641 cgtgccgagg tctccatctc tttcgtggat cgactcggta tccggacgct gtcttcgatt
    92701 gaccttaacc gtcgctgtgc catgactgaa cctccctgtt gagtccgagt gagtggtgtt
    92761 cttcccaaga tgagcgcatc acgtcagaga tgcgcgtacg catggaatac cgagatttgc
    92821 tcgcagcaac tcctcgacgc gattgtgcac gagcggagcc tgatgacgtg gacgagcgtc
    92881 tattggcgga gaaagagtac ccaacgcgtc tcgcatcggt tcgacgacgg tcaagatcgg
    92941 cgcttgtggg cagaggacgt tgagggctcg aagagtatcg agttcagcgg tcccccgttt
    93001 ctcatcgcaa gagtccgaac taagcaggat ggcctccggc tgtatctccc cgagctcggt
    93061 gagcatggat tgaaggtctc gaacgagaac tgcatggtac ccattggtag cgaggcaatc
    93121 cgccagtttg agtccgaatt gccattcagg atcgacgatc atgacacggg atcttccggt
    93181 ctgtttggta ctgttcattg tcgcgctcat gtgtcgacct ccctcttatc tcccgtacga
    93241 acggccggtg tgctgccggg tttccgctac acgcctgtcg ggatcgaagt ggtgcaaggt
    93301 gtgtgcgtgg acagacgcga gagaaaccag gtattggcac ccagcgcgcg gttggaggga
    93361 gatcgttcct tcccgcgaca gggcatcgac tgcgatgaat atctggttcc aggtgcaggc
    93421 ggggaatcta taggccagtg catccaatgt gcaggggccc tgctgttcca gctctcggat
    93481 aatgagggcc tctgtctcat agagctcggt catgtcatcc ccctctgacc ggtcatcgtc
    93541 tgagttgtaa ggagggacca gcccaaccat gccacccggt cctctcttgc gaatgacggt
    93601 gcgaacggtc tcgcaaccag attgcaccgt cagtgctatc tgtaccggaa gcaggttccg
    93661 aaaagcatag ccttttacgc aatttacgcg tcgccgtttc ctgtgcggcg cgtgtcgacg
    93721 tttatgctac gaagggagct tgtagagcgg gaacggatag gcaatgatga cagagaggaa
    93781 ggatctcacc tgatgagccg gttaacgaga cgatgttcta tcgcgtaatg cgtcaactcg
    93841 gtgttgttct tcagtttcat tttttcgagg atgcgcgccc gataggtatt gatggtgctg
    93901 acaccgacgc acaaatcatc cgcgatgtct ttcaagcttt tcccccccgc gattaaacac
    93961 atgacctggt actcgcgatc agacaattgt tcgtgcagcg gtttctccca atcactctcc
    94021 agattcaggg ccaggagttc cgccactgcg gcactgacga attttccgcc gccgaggatc
    94081 ttccggagag cttgcactaa ttcgttcggc gcactgtctt tcgacaggta gccggccgcg
    94141 ccggctttca acattcgtaa tgccagctgg tcttccggat aaccgctcag gacgaggacg
    94201 gggagtttcg gccgggcctg tttgagctct ttgagcgcat cgagtccgct cttcccgggc
    94261 atgccgacat cgagaatcac aatatcccag ctgtgcttcc ctacctgcgc gatcatctcc
    94321 tccgcgtttt gagcgtcgcc gaggacggcc ccatggaact gttcgctgag aatttgtttg
    94381 acgccctgtc ggacgacggc gtgatcatcc acaacgagaa ttcttgtcac aacctgttcc
    94441 tccttccgct atcgcgcctg agaggttgaa cgcgcagtgc cccctgttgc gttccggcgc
    94501 cgggccgact atggctgacc aagaggaatc cggaccctca ccgccgtgcc tgtcccagga
    94561 ctccctgtga cgatggtttc acctcccaga agcagggctc gctcccgcat tcccaccagg
    94621 cccagggaat gcgggtcggc caattcagcg tcggtgacgc cgcgcccgtt gtcgcgcacg
    94681 tcaagcacaa ggctgccggc ctgctcttga agggtgatat ggacgatcga cgcctgggca
    94741 tggcgagcca cattagtcag gatttcctga aaaattctga acatggccgt cgaaccggca
    94801 tgagacaacg tgatagtgcg cagatagacg tcgagcgtgc attgaatccc tgtgcgtgct
    94861 tgaaattcat gggcctgcca ttcgatggca gggatgaggc cgagttggtc gaggacgaca
    94921 gggcgtaatt ctgtggcgat gcgttgaacc gactggatcg tgccatcgac cagcgtggaa
    94981 atagactcca gccttttcat catcgcagga gagacttcgg agatctggtc gcgtaacagg
    95041 cagaggtcaa gtttcacgcc tgtcaacgct tgccccaatt cgtcgtggat ctcacgggcg
    95101 atcagaattc gctcctcttc ccgaactgat tcgagccgcg cggtcagttt tctgagttga
    95161 tcgtgcgaga gggtgagctg ttgttcggcc cgtttgcgcg cggtggtgtc gagaaacacc
    95221 accacggctc cgatgatctg ctcttgttcg atgatcggaa acgaagagta ctccaccgga
    95281 aaatccgtcc cgtccttgcg ccagtacact tcgtcatcca ccttgcaccc tgttccgcgc
    95341 tcgagcgtct cttgaatacg gcaggccgca cgggggtatg cggtgccacc cgtgaaggaa
    95401 taatggatgc gctcatgcat atcttggccg aggagctcgt ccgggagata gcccagcatg
    95461 cgcgctgcag ctgaattgat gaatgtgcag cggccctggc ggttgatgcc gtaaattcct
    95521 tccccgctgg actccagcag cagcaaacga tccttggcga gctgttgtcg ggcttcttcg
    95581 gctcgctttc gatccgtaat atcggttgcg atgccgcaca gggcgtaaca acgattctgg
    95641 gcattgcgga ggggaaactt gacgacgatg ctggtgtgga ggccgtcgtc gtgcatgacg
    95701 acctcctcga attccattgg ggccccgctg tccagaacct tgcggtcgtt cgcgcgaaac
    95761 acggcggctt gctcaggtgg aaagatctcc tcgtcggtct ttcccttgag atcatcgtgc
    95821 gtcagatggg atacgatctc gaactggcgg ttcgcctgga gatagcggcc ttcgagatcc
    95881 ttgatgaaga tcaacgccgg gctatggtcc atgatcgcgc ggaaccgctc ttcgctgtcc
    95941 cgcagtgccg cctcggccag cttgcgttcg ctgatatcgc gtacgatgcc ggtaatgaac
    96001 tgttgtgact tgatcgccca ggtggcaagg ctcaattcca gtggaaattc cgtcccgttc
    96061 tttttgagcc ccctcaattc gaacatggcg gcatgcatgg tcagccgtcg ggcggcagcg
    96121 actcgttgga ctcctcgttc atgggcctcg cggaatcgtt cggggataat tcgagtcatc
    96181 ggttgaccga ccatctcctc ggccccatac ccgaagattt tctctgcgcc gcggttccag
    96241 aaggccactc gtccctgagt atctatcaga atgatcgcat cactggtcga ttgaacgatc
    96301 gaccgcaagc gcccttcgct ttcggccagg gcctcttctg cccgtttacg ctccgtgatg
    96361 tcacgggcca ccgcttggaa gccgaccacc ttcccccctt ccaggatgac ttgcacgttc
    96421 tggccgatcc agacttcgct tctgtccttg gcgagggaga gaaattcgta ataggtgctg
    96481 ggctgttgcc ggatgaattg ccgcccgtaa aaccgctcgg cggccggccg atgatcggga
    96541 tgaatcaact cgatgaagcg acgccccaac agttccggtt ccgtatattt catgatgcgc
    96601 atggcgaccg ggttcacaaa ggtgaaccga cccgatgcat ccgttctata gatgatgtcg
    96661 ttggctttag tgaccagcag tcgataccgt tcttggctgt cccggagcgc ttcatcggtt
    96721 cgcttcgcgg cggtgatgtc tcgaaagacg gcaacccctc cgctgaggtg ccccgtgtca
    96781 tccatcaacg gcctggccgt aacactgagc caggcttcgc ccggccaatg cggccggtga
    96841 aaaaagatca aggcatcggt cacggattct ccgcggatcg ctcgtgccag cgggaggtca
    96901 tcggtcggga atggtgtgac cctgtccggt agaaacagcg cgtaatgctt ggaccattcc
    96961 ccgattccta tgtcggcagg accgcttccg atgatacgtt cggcggcctg gttccatact
    97021 tggaacgttc cacgttcgtc ggccatcacc acgccttcgg ccatgttgtc cagcacggac
    97081 tgaagaagac gtgttttcag ccgcagttct tcttccagcc gcttgcgttc gacggcatac
    97141 ttcagggcgc gtgccaggag cggggcagtc gtctgtgttt tcacgagata gtcctgggcg
    97201 cctgcctgga tgagctggat tcccaattcc tcatcctcca gtccggtcac caacacgatc
    97261 ggaattccct gcgccgccgc gtgcatcctg accagggtct ctgccccctg gctgtccggc
    97321 aacgagagat ccagcagaat gatgtcgaac gcggcctgag tcaggcatcg gatggcggca
    97381 tcgagccggt cgacgtgcgt gagggtgagc cggccggcgc cggtttccgt caggagttcc
    97441 ttcagaagac gagcatcgcc ggcattgtct tcaaccagca ggatatgggc cggaccggtg
    97501 ctcatctggt tccgttttgg ggtggaacgg gtaacacgac gatcccgagc cagaaatctt
    97561 caatggatcg cactacccga atgaactgat ccaagtccac cggcttcgtg atgtagcaat
    97621 tggcgtgaag gttgtaactc ttcagaacat cctgctcgtc ctctgaggtc gtcagcacga
    97681 ccaccggaat gcgtttcagc ggctcctcgg ccttgatttg agccagcact tcccgaccgt
    97741 cttttcgagg cagattcaag tcgagcagga tcaggtgggg gcgcatcgcg tgggcatagg
    97801 gaccctgtcg gcgcagatag gtcaaggctt cctcaccgtc cttcagcacg gtcagatggt
    97861 tcacgacttt ggcttccttc agtgcttcca tcgtcagccg aacatcgcct ggattatcct
    97921 cgaccagcag aatttcgatg ggcttcacca tctcaggtac catacaactc ctgctccttc
    97981 cttccgtcag cgattcgggg ggtgcgccga ggtgcccggt tcctcatcgc gcatggtgaa
    98041 ataaaatgtc gctcccttgc cgagttcgga ttccacccat atgcgtccgc cgtggcgttc
    98101 gacgatcttc ttgcaaatgg ccaggccgat accggtcccc gggtattctt ctctcgtgtg
    98161 gagccgctgg aaaatgacaa aaatccgatc cgcatattgt gggtcgagtc cgatgccatg
    98221 atcgcgaacg gaaaacaacc attcaccatc ggtttgtttc gcggagatgt gtatgcgcgg
    98281 cggttgtgcc ccgccgaatt tgacggcatt gctcagcaga ttctggaaca attgcgccaa
    98341 ttgtttgtca tcgcccatca ccgccggcaa cgggtcatgg gtgatgatgg cctggctctc
    98401 cttgacggcg tcggtgagat tgttcagcgc agcctttaga accgtctcca tggctgtcgg
    98461 ttcgaattgt cggcctgccg tattgactcg cgaataggcc aagaggtcca ggatgagccg
    98521 ttgcatgcga ttggcgccgt ccacggcaaa ggcgataaat tcatccgcat ccgcatcgag
    98581 tttccccttg tagcgcctcg caatgagctg cgtgtagctg ctcaccatgc ggaggggctc
    98641 ctgcaagtcg tgcgaagcca cataggcgaa ttgttggagg tcggcattgg agcggcctaa
    98701 ttccgatacg gactcctcta acttttgccg ggcctcgacc aggtcattct tctcgcgtcc
    98761 cagttcccac gagacccaga tgccgtagcc ggtgaggggg aggatgacca gcaatccaat
    98821 ccctcccacc atccagatca gtcgatcgat cggggcatag acctgatcgc ggtccaagcg
    98881 aaacaagacc agccagtcga atccgggaaa gttggcatac cctctggtcc aggcatagcc
    98941 ggtgacgact gcgtttcctc cccggcgatg ggtttcttgc acgaaaccgg ggtgatctcg
    99001 atgaatgacg gcttgggctt gtgaaggcaa atctatcttt accggattgc cgttcagatt
    99061 cgagggtcga tgtttctcgc tgaggaccgc cccctctcgg tcgagcagca gccagtcgta
    99121 ggccaactct ccgtatcgca gtctgccttc ctgctcgaaa atcgattgaa gattctcaaa
    99181 cggcactcgt gtgaccacta ccccatgaaa ctctcctttg ggaccataga taggtgccgt
    99241 gaatccgata gccatctttc tctgcgatgc cggggagggt cgcatgtctt cgaagtgaac
    99301 cttccctgtc ctgcgcacca actcgaacga ctcggggttc aagccttgcg tgccctgcgc
    99361 gggcagcgcg tcggtggcgg ccgtgaggtg tccgttctca tcggcgaccc ccagccagga
    99421 gtaatattga tagagttgtt tgtatgccag gagccgggcg gtcttttcag ccgaggtgcc
    99481 gtctcggagt atgccgtcat tcgcaaacag ctggatatcg ccgaaccgtt cgaacatgac
    99541 gcggtcgagc gcatccgcga ctgcggcggc attcatggcc aattctcgtc cccgttctga
    99601 gaccagcacc gttcgcagga acgacaacgc atagagtccc agtgcagtag cgatgacaaa
    99661 acagagaagc aggaccagcg ggaggccccc ctgggtcgat ctgcttctgt ctgataccaa
    99721 gccagccttg gagggcgcgt tgttcagcgc aggttccatc gcactcttgt gtcaggggtt
    99781 taaaggggaa gaccttagta tcataccctg ttcacggata cagttccatc cggcagatag
    99841 gggcgtctca tcggtgaatt gccgcctgcg aacaaggtgt gatgtgctca cgtgattgac
    99901 tcccgccttg tctccccagt aggttctctt cactgtcggg cgcaggacct caccgctatg
    99961 ttgtctattt caacgcatgt gaccattccg gacgaggaga tcgaattgac cgctgtgcgg
   100021 gcgcaaggcg caggcgggca gcatgtcaat aaggtttcct ccgcgatcca tcttcgattc
   100081 aatattctcg cgtcttccct tccgccgttc tacaaagagc ggctgctggc cttgcgggac
   100141 catagaatct cgcgtgacgg caccattatt attaaggcgc aggactcacg gagccaggag
   100201 cgcaaccgtg cgttggcgtt ggatcgtctg caggaattga ttcagtcagt ggcggtgctg
   100261 cgtccgccgc gtcgtccgac gaagccgacg aagggatcga cccaaagaag gctggacagc
   100321 aagaccaagc gggcgcgtct gaaatcgttg cggggaaagc cggacgagca gtagtctcac
   100381 cagacgcagg gcaaaaaaga aggggcgctt cagttgaagc gccccttcgg agacgagacg
   100441 aatctcgtca cttcttagaa gcggctgcgt ccgccacggt tttcaccgcc gccgaaccgt
   100501 ccacccccgc cgccgccacc ggagcgagct tcttgcggct tcgcttcatt gacggtgagc
   100561 tgccggccat ccatgtccga gccgttcaac gccgtgatgg ctgccttcgc ttcttctgcc
   100621 gtggacattt ccacgaagcc gaagccgcgc gattgtccgg tgaacttgtc cgtgatcacg
   100681 cgcgccgatt caacggtgcc gtgagcggaa aacaagttgc tcagttgcga ttcagttgca
   100741 gcatacggca gtccgccgac atacaattta gtacccatag gggtctctcc ttttgaatag
   100801 tgacattgtc ggcgacacga gaacagacat atgaggggaa tgagcgggcc gaagacgcga
   100861 tgattgaggc gacttgggtc tgacttccga tcaacttctc ggtaacaaac aacatcggtg
   100921 atgagccatc cggaaccatg attctcatgc ggcccgtcat gagatcacac tgcatcctaa
   100981 cagatgaata gggggattgc tacacgattc atggacaggg aaaaaatagt ttgtatcggt
   101041 cggatgcggt cgatcgctgc acggagagag gccacgcgcc tccggcctga tttctgtggt
   101101 ggtgatccgg tcgttcctcg gcgtggcaca caatgggtgt ggttcagtgg ccggaagagg
   101161 ctgtccggag cgaggatgcg agtcctaact tccataaggt gtagatcaac cacttcgacg
   101221 ggtcaaaatt gtaccacagc ggcccgttgc gatagtcgct cggataggtg tggtgatagt
   101281 tgtggtaccc ctcgccgaag gtcagcaatg agacaaacca ggagttgcga ctggaatcgg
   101341 ctgtaccgta gggctgctcg ccccacaggt gacagacgga gttgatgcag aaggtggagt
   101401 tcagtacggc aaaggtgcgt ccgacgccgg ccagcatgaa gccaccgatt ccgcccttcc
   101461 agccgttatg caggaagccg acgacaaagg ggagcgccag gccgacgagg acgatcaccg
   101521 catagtagcg gtgttgccac atcgcgaccc ggtcctgcat gacgcgggag ccgaatcgtt
   101581 catcgttgca tggcactgga tcgagcagcc agccgcagtg gctgtaccag aacccccgtt
   101641 tggcgttata gggatcggcc ggatcgtcgc agtgcgcatg gtgtcgcaaa tgatcggctg
   101701 tccatttcgc gccggagttc tggagcgccc agccccccgc caccaataga cagcccttca
   101761 cccagtcggg acattcgaaa ctgcgatggg tcagcagccg gtggtagccc acagtgatcc
   101821 ccagtccggt caccacatag aggatggcga acatcgtcca atccagccag gagtagccat
   101881 agaccagtcc atagccgggc accgcgatca ttgaacctgc gacgactgac cagaatagga
   101941 gataggtcca gaacttatgg tgaacacgat ggacaatgtc ggttggaacc gaactcgggg
   102001 cgatttgttg catcccgcta caatagtcaa gcgtgcgggt tttcgcaagt gtctggtcgg
   102061 cgccgtacga tcgatggacc gcagatttgt tccggccgct cttgcctggg gccagcggtg
   102121 gccgagggtg accgtcaacc gttttcctgc ggagacgttg agtcactgtg tcggtaacag
   102181 gctccacgcg cagtcatcgt gggtgcggaa ggagagcgga cctagtgtgg tcatttattt
   102241 tcgaaagggg ggagtatgcg ggttgattgt ggggttctaa gaaaggcgat ggggattgtg
   102301 gtcttgagcg gactggcggt cgagctgttt ggcttctatg aacccgcctg gtcgacatcc
   102361 gatccagcaa cggcagccgg tcaggaccaa tccgctgctc cgcccgagtc ttccggcagt
   102421 cctggagcag gggctaagga aaaaggcttt gggggccatc gcatgcggaa ggtctgcgcc
   102481 gaagatgtga aaaaactctg cccgggcgtc aaagcgggag aagggcgaat cgctcagtgc
   102541 ttgaaggcgc atgccgcgga actctcacaa acctgctccg aaatgattcg gcaacgcggc
   102601 caacaccggt agtcgcctcc ggcttggggc gggtggagga gaggtgtcgg gaaggtggcg
   102661 atcggtcagg tcacttccgg caccgtctct ttttgttccg ttgcgtccgg ttggttttct
   102721 caatttattt ttcgaaccgg cgcaggtgcg atacagtggc catattacat acgggagggt
   102781 acgtatggat ggcactacgg gacagggaca gttctccgtc gatttgcggc agcatccgcg
   102841 tttgcgcgtc tcggcaccgt tcgtctgttc cttttcccgc cttggcttgg ccaaatggct
   102901 tgggcgcggg ggggacggca tcggggtcgt gttcgatgtg tcgatgcgag gggccaaagt
   102961 gatgagtgag tccggtattc agcgcggcga cagggtctcg gtgacgcttg ctttgcccaa
   103021 tcaggtgtcg ccgatgacgg tggaggaagc gacggtgcgc tgggagaaag agcaggtgta
   103081 cggattggag tttgtcgatc tgtcaccagt ggccgagatg cgcttacgaa agttcattac
   103141 catcgcgacg aaaccggggg cataacccca cgttacgggg atctgcccgg cacagtagcg
   103201 cgaaggcgca cagaaccgcg catagcctcc atcgggccgg cattgcggtc ctggtgcatc
   103261 agttccaacc gcgtccccct cgccagctcc cgttgtggcg attccccgca tcgcttcgag
   103321 attgcgctcg ccgctccgtt tccctacaat ggcttcccca ttcggccatg tccagtacga
   103381 acacacgaaa gaagcggcag cggccgcata gttcctacca tccgctgtat tggccgacat
   103441 gggtcggtct cggattgttt cgcctgctgg ccctgtttcc ctaccgctgg caacttgcat
   103501 tcggacggca atgcgggcgc ttgctgcatg gtttattgac caagcggcgc gatatcgtcc
   103561 gggtgaactt ggccgtttgt tttccccgac aggaaccggt tgaacgtaac caggtcgcgc
   103621 tccattgctt cgagtccatg ggaatggggg tgctggagat cgcgatggcc tggtgggcga
   103681 aggatcctcg cacctattgc gagtgcacca tcaagggatt ggagcatatc cgcgaggcgc
   103741 tccgtcaggg acgaggcgtg ttgctctgcg gcgcccattt gcatgcggcg gaactggccg
   103801 gtcggttcat ggcgctggag cagccggtgg cgatcgtcta ccggccacag aacgatgtgg
   103861 tagccgaaac cgtcgcgaat cgatgccgga gccggtacta ttccgagttg attcagcacc
   103921 gggatatgcg ggggatcctg cgagccttag ccaggaacca ggtggtgtgg tatgccccgg
   103981 atatcgatgc aggctccaag cgcagcgtct tcgccccgtt tttcggtgta caggccgcgt
   104041 ccctgacggc cacgtcgcga ttggcgaagg tcagcggtgc cgcggtggta ccctgctttt
   104101 attatcgaag agccgatgga tccggctatg acattgaagt cgggccggcg ctcgagcact
   104161 ttccgtcggg tgaaatggtg gaggatgcca cacgtatcaa ccggctgatc gaaggtgcgg
   104221 tccgtcgagt tccggagcaa tacttctggc agcatcgccg cttcaagagc cgtcctcccg
   104281 gcgagccgcc gatctatcgg cgctaagtgg ctttccatca atgctgtcgc gcggtcgtgt
   104341 atattcgact cggggttctg gcaaaatgat gagaagtatt cggcgtctcc cgctccttgc
   104401 gatatactga tcctggctga atgcagtgca cgatcgcacc atccgctccg ccatggatca
   104461 atccaacaac ctctcttccg cgcgcattga actccggcaa caccagcggt gtgttgtgcc
   104521 cccggcatgc ctgctctcgt tcgcaccctt tgctccgacc atcagtttta acggtgatgc
   104581 ggaaggtgaa ggcgtcgtca tcaacctgtc cgccggtggt tgcaaaatca gcagcgacgc
   104641 gggtgtgact gtgggagatg cgatgtcgtt gatcattctc cttccggggg aactgtgccc
   104701 gaccaccata gatctggccc tggtgcgatg gggtcgggat ctacattttg gtgtggaatt
   104761 tgtctccatg gggacaaccg aattcaatcg gatccgccag tttctcgcat ccaccggtcc
   104821 cgaatagggc cggtatcggg gccgatgagg ctgggggggg tgtgctgaga aaggagcttg
   104881 ggtggaacag acgaaaacgc cgcgaaaatc tcgtcgggtc gcaaccgatt gccgattgga
   104941 atttaccggg gaggatgagg tggatgggga ggcggaggtg ttggatgtct cctcgaccgg
   105001 ctgtatggcc aagtcggata acaggatgtc accgggactt catatgaagg tggctttatt
   105061 tctgtcggac gggcatgaat ggccggtccg agtggatgaa gccgtggttc gatgggcgcg
   105121 aggcggggag ttcggtgtgg agtttctggc gatgcgccct gcccaactcc agcgcatcca
   105181 gcagttcgtc atcaaggccc ggccgttgtc ataggcctcg tacaacgttc ctccttcctg
   105241 ttgttgctcc gccgctattt gtccacgctg accagcccac acggcatctc ctctcgcctg
   105301 cgccggttcg agacgtcgcc ggcagggcga ctcattgctt tgtttccgat ccttccccta
   105361 caatttcgcg ggatcccaca tgaatattgg ctcggcccga cggtgttgct ccatggaaaa
   105421 ggcggctcac aacgcatgat cgagtgggtg gggcgcaagg ccatcgcggg gtatacgtac
   105481 ctggcggcgc tggcgacact ctttacgcaa gccgtgctgg acctcgtgct ccccacacgc
   105541 caaggccgtg gggagacgct gcgggtactc gtcaggcaga ttctgttcac gggggtcgat
   105601 gcgttgcccg tgacgaccgt catcgcgctc ctgctcggca tcatcatcgt cacgcaggcc
   105661 gggacacagc ttccgaggct cggggccggt ggcctggtgg ggagcatcat cgtcgtgacc
   105721 gtcattcgcg aactgggccc gttgattacg gcgttcattg tcgtcgggcg ctcaggaacg
   105781 gccatcgcga ctgagttggg caacatgtcc gtgacacgcg aggtggtagc gctccggctc
   105841 atggggattt ccatcagccg atttgtcatt atgcctcgca tggtcggcat ggtcctctcg
   105901 atgctctgtt tgacgctgta tttcgatgtg gtggctgtgt tggggggcta tctcatcgcc
   105961 gacgcgcaac tgaccattcc gttttatgcg ttcgtcgaga gcatcacgaa ggcgttgtcg
   106021 acgacggatg ttctgatgac tgccatcaaa ggattgtcat tcgggtcggc cgtggcggcc
   106081 atctgttgtt accatggcct ggctgtccgg tcctccttta ccgaagtgcc gcagcaaacg
   106141 acccgggcga tgatcaattc atttgtcctc tgtctgctga tcgatgtcgt cgtcacggtg
   106201 ccgttgtacc tatgaatgag gcagccctcg ttctggagca ggtccggacg ccgatggcgg
   106261 gcagtcctca tgttctcgat gtcgatctga ggattgccgc gggcgagttc gtcgcgctgg
   106321 tggggccgag ccgctcgggg aaaagcctgg tgatcgagct ggcagcgggg ttggtgcgac
   106381 cgcaagccgg tcgggtggtt ctgctggggc atgagtggaa cgataccccg gagagtgagc
   106441 agactcccgt gcggcttcga gtggggactg tgctgctaca gccggggttg ttgagcaata
   106501 tgaccctctt tcataacgtg cttctgccgc tccggtacca tcatggagcg atgccggagc
   106561 ggagtcggga gcaagccgtg atggcgcagc tggaacgact gggcgtcgcg ccgatgcgag
   106621 accggtttcc tgccgagctc aatcaaggcg aagtacgtag agcggccatt gcgcgggctt
   106681 tgatgttgga ccccgaggtg ctgctcctgg acgatcccgt ggccgggctg gatgccgaga
   106741 tggtgttggc gctcaaggcc tgcgtcgaag aacgtcgggc tcaccatccg ctgaccgtgg
   106801 tcgcagcctt acgggccttt tctcccttta tcgaagcggc ggatcgtctg gtcgtgcttc
   106861 gcgacggccg cgtggaagcc gatggggccc gtgatacggt ggcccgcacc gttcctgaga
   106921 tgctcagaaa gtatgtggaa tagaccgcgt tctgccgagg cggtccagca agactgaggg
   106981 gtggggggat gcactattca catcgcttat ccagcgggcg gttggctcag attgtcggca
   107041 cattcgtgct gattcccgtc ctgatcctgg cggtggccgg cttctggatg tcgaagacgg
   107101 aacatctctt cgagccgaag tactacttga tggccagcct cagcaagtcg tacggtctcg
   107161 agccgggcgc gccggttgtc gtatcgggga ttcgggtcgg gcgggtgaag caggtggatc
   107221 tcaacgatcg cgggacggtc gatctgacgc tgcaactgtc ggcccgctac caggacatgg
   107281 tgaagggcaa ttcggaactg catattacca agagcggtgt cgtggtcggc cagacccaag
   107341 tcgagatcgg ggcgggtacc gccagcagcc ccgtgttgac cgatggcgcg atcattcgcg
   107401 cggttgagcc gagagatatc ggggacctgc tcaatgaggt tgaaccggtg ctgatggcag
   107461 tgaagcagac gttgctgcgt gtcgaaacca ttacgcagga tctgcagggc gggttgaaag
   107521 ccggcggcaa agccctggag caggtggcgg tggcgacgca ggatttgccg gcggtcgtgg
   107581 catccgtcca acgcaccatc gcctcggtcg agcaaacggc gactgcgctt cccgccatga
   107641 ccggctcagt tcagcgcacg ctggcggtgg tggatcgtgt cacggcagac atgcagcgga
   107701 cgaccagtcg cttgccggct atcttggaca gtgctcaatc cacactcacc agtgtgaagc
   107761 gcctctccga ttccgtggag gaggtgagcc atgagctgct gccggtcgtt cacacggcgg
   107821 aaactacgct ggcagacgtc tccatcctag tgcgtggcgc aaagcagacc tttcccttta
   107881 accgattcgc gcagaacgcc gggccggccc cgaggccgga ggccgccctg tcacccacgg
   107941 ctcccccgag tttgcgcggg gatcacgtgc gtcgatgatc gtcagagggc atggttgttc
   108001 ctcaggcttc tggggggtga cgttatgtct cctgctcggg tgcaccgttg cgccgccacc
   108061 tcctcccgtc cccgcgccca ggcctctaag cgtacaagtc gagtccttgc gcttacaagg
   108121 agaccgttgg atgatccggg gcgacctggc acgtgccggg gaagcctatg aagaagctcg
   108181 ccggctggcc gaaagtctgg atgatatgaa aggactcgtc ggggccttga acgatcttgg
   108241 gtctgtcgcg cttggccgtg gggccgggcg tgaggcgatc cagcttcatg ggcgggccgt
   108301 ctccctggcg caacaattcg gagagacgga cctcttgatc gctggtctcg catcattggg
   108361 ggcggcagaa tatcaagagg ggcggactga ggaggcaggc cggcgctatc aacaggcgct
   108421 ggatctcctt cagcgggcag cagaccagcg taccgaagcg atgctccgga ataatctcgg
   108481 actgatccgg caggcgaccg gtgatgttgg acaggcagaa caattgttcc ggcaggcgat
   108541 ggccctgaat caggccgcgg ggcaccccgc tgccgaggcc agcaatcatg tgaacctggg
   108601 gattctggcg gaggagcgcc gcgagtacga agtcgcggaa cgggaatttg agcgcgctct
   108661 ggagttggac aagactgcgg agcgccgggc ggagattgct gccgatcttc tgcggctagg
   108721 ccgggttgcg gaacggcggg gatttcccga tcgtgggctg gcctattccg agcgggccta
   108781 ccgtagttat ctggcgcaag ggaatcaggc tcaggcgatt gcggcgatca cctttgcgtt
   108841 ggactgtgcg aggagattgg ggctggttga ggaggttgct cggctggaga aggagctgat
   108901 ccgtctgacc ggttcccgtt ccgggcgata gcgtacgctc cggttgtcgt ccgccgatgt
   108961 tacttaatgg cgacggcttt gacttctttg taggtcgtgt ggccttgcat gactttctgg
   109021 atcccgtcct ggaccagagt cgtcatgccc tccgccatgc cggccttcag catttcgtcg
   109081 gttttcgctt tgttttgaat caacttcttc atttgatctg tgcccagaag cagttcgtgt
   109141 agcgccacgc ggcccttcag ccctgtccga ttgcaggtgt cgcagccctt gcctcgatag
   109201 agcctgaagt tggcgtcgaa tgtcacgccc agcgtgtccc agtagtcagg gccatatccc
   109261 aaccgcagtt cttcatactc ttccttcgag ggctgatacg cttccttgca gtctttgcag
   109321 atccgtcgcg ccaaacgttg ggcgagcact cccagcatgg cgtcggcgaa actgaacgag
   109381 tcgcagccca tgtcgagcag acgggtgatg gtttccaccg cgctgttggt gtgcaaggtg
   109441 ctgaggacca agtgaccggt cagcgaggcc tcgatgcccg tgtccgcagt ttctttgtct
   109501 cgcatttctc cgaccatgat gacatccgga tcggcccgga gaaacgcgcg catggcggca
   109561 gcgaacgtga agccgatctt ggaatgcacc tgcacctgac gaagtccgta ttgtgtgatt
   109621 tcgacggggt cttccgccgt ccagatcttg atgtccggcg tattgatgta acccagcacc
   109681 gagtgcagcg tggtggtttt gcctgatccg gtcggtccca cgcagagaat gatgccgtac
   109741 ggcttttccg cgatactttt gatttcgcgc agattccgct ccgaaaatcc catcttgtcc
   109801 accggcagcg gttcgctcgc agccagaata cgcatgacca cgtcttcgtt gtagccggcg
   109861 gttgggatgg tggcgacacg cagctcgatt tccttgttct cgccgatctt gaatttgatc
   109921 tttccgtcct gcggttttcg tcgttccgcg atgtcgagac tcgccatgat cttgagccgg
   109981 gagacgatgg cgcggcgata gctcggtgga attttcatgt actcgaagca gtcgccgtcg
   110041 acccggaacc gcacgagggt atcgcgcttt tcaccatagg gctcgatgtg aatatcggag
   110101 gtgccttgcc ggaaggcgtc ggcgatgatc tggttggcca ggcgcacgat ggcgttgtcg
   110161 ttctcgtcga gtcccgcgct ggaggcatct tcctgagcct caagttggga ttcgttcacc
   110221 agttcgccga gaatgtcgga cacattctca ttcatcttgt tggtgatctc cggagtcccg
   110281 gtcgtgctcg caaggaagag ggagatatcg cgacgaagac tgaccgcaaa gcggatgttc
   110341 aggccgggga aggtgcgttt gatgtcctgg acgcgatcca gatcgccggg atcgtcggtc
   110401 agaatttcaa tcgccgcgcg gtcccgcttg agcggcatcc agtgattttt cttgaggtag
   110461 tcgacattga ggtttttgag cagttcgata tcgacgattg tccgctcgtt gtattcgatg
   110521 taggggcatt tgtgaaaatt ggcgagcgac ttgccgagtt ccgctttggg tactttatat
   110581 ttttcgatga gcagcgactc gatgtcggtg gtcccctttt ttgcctccgc gagcgcattg
   110641 tccaaatcgt gttgcgaaac ttttccgttg gtcagcagat aatcgaattt cgagggaggc
   110701 tttttcgacg tcttcctgag cgcgtaaaaa gccgttccta atgccttggc gatctcggcg
   110761 acacattctt cgtctttgcg cgtgaaccgt ccgccgctct tcttgttgag cagctggatg
   110821 acacccatga gatatttgtt ctcggcgacg acgggatagg tgagcacctg tttggttttg
   110881 aagcccgtgt ttttgtcgta ggaggcatcg tgggtgagcg aggggtggat cgcactcagt
   110941 tcgaccggac tgtaggcgtc gccgacattg acggggcgaa ggtatttggc acagaacccg
   111001 gcaagactct gttcggtgat cggggtgcgg acttcctgaa cggtatcgag atggggaatt
   111061 ttggagaaga tctctttctt ttccgagtcc accacaaaga gcgtgagatc ttccgcgtcg
   111121 aaacagctca agatttcatg gcgcagatcc agcaagatct ggtcgacatc ggacgcggcg
   111181 agaatttgcc cgctgatccg cttgacgttt tccgcgttgc cggatttgag agtcgcgtcg
   111241 cctagaccgg gcagcggggg tttgggctgc atcaagttgt ctccaaaacg ctcgcgcaga
   111301 gggcggttgc ggtgtcgacc gggatccgaa cgccgggctt cttccgtgtg agtcagtgta
   111361 tcgcaacgcg cagtcaaggc aagcaaaaga cactttctca aaccgattct gccggacgtg
   111421 tgaggacgtg tccgcaggag tgaagttctg tcgggggagt gaacgattga accgtctgat
   111481 ggagctagga agcggctttc aggaatgagg acagcaggga gaccacctgg tcgggagcga
   111541 ctttttgagt ctcgccggta tgacgaagtt tgagctcgac ctgaccttgc gccaaccctt
   111601 tctccccgat gaccaaatga tagggtgccc caatcagatc ggcgtcgttg aacttgaccc
   111661 cggcccgctc gtcccggtcg tcccacaaga cttcgatacc ggcctcctgc atggcgcgat
   111721 agagggtctc ggctaactgc gtgactggtt gagactggct gagcggcaac agggtgacat
   111781 ggaagggcgc gatgggaacc ggccacttga tgcctttcgc atcatgattc tgctcaaccg
   111841 aagccgcagc ggttcggctc acgccgattc cgtaacagcc catgaccgcc agctgttcct
   111901 gtccctgcgc atccaggaag gtggccttca tcgcctggct gtactttgtc cccaacataa
   111961 agacatggcc gacttcgatg cccttggccg tcttcagggt gccgtctttg cggggtgaac
   112021 aatcgccggc gcgtgcatta cggagatcgg caaactgatc caccttgaag tcgcgctccc
   112081 agttggcgtc tacgaaatgg gtgtcggcct ggttggcgcc gacgacgaag ttggccatgg
   112141 ccttcacggc ccagtcgccc atgatgcgga cctgcttcaa tcccaccggc ccaacgaatc
   112201 cgaccggcgc acccgtgagg ccgggaacca gctcgggttt caggagctca atctccggca
   112261 cacccaagag gcgtttgacc ttgatctcat tcacatcatg atcgccgcgg accaggacgg
   112321 cgaccgtctc tttgcccgtg ctatagagca gcgtcttgac cagttgtcgt ggctcaattt
   112381 tcaggaaggc agtgacttcc tccacagttc gtcggttcgg ggttgagacc ggcgtaagcg
   112441 ggcggtgagc cgaattgtca gcggtatccg gagggaggac ttcggcacgt tcgacgttgg
   112501 cggcgtacgt gccgtcttcg ctatagacga tcgtctcctc gccggtctcc gccagcacca
   112561 tgaactcatg tgacgaagtg cccccgatca acccggtgtc ggcttccacg ggacggaagg
   112621 tgaggccgca gcgcgtgaag atgcggttgt atgcgtcgta cattttttga taattcagtc
   112681 tggctccggc ttcatcccgg tcgaagctgt acgcatcttt catgatgaat tcgcggccgc
   112741 gcatcaaccc gaagcgtgga cggatctcgt cacggaattt cgtctggatc tgatagaaat
   112801 tcaacggcat ctgccggtat gagcgaactt cccggcggaa gagatcggtg atcacttcct
   112861 cgtgtgtcgg cccgaggcag aaatcgcgct cgtgccggtc cttgaaccgg agcagctcct
   112921 tgccatagaa gtcccagcgt ccggtttctc gccacagctc cgccggagag gcgacgggca
   112981 tgagcagttc ctgcgctccc gcccgattca tctcttcgcg aacgatgcgt tcgattttac
   113041 ggagcactcg caggcccagc gggagatagg tgtagattcc ggccgcgacc ttccggatca
   113101 ggccggcgcg cagcatgagg cgatggctga ccgtttcagc ctcgcctgga tcttctcgca
   113161 atgtggggat aagggtttcg gaaacgcgca tgtgggttta tggggaaatc agccgctcga
   113221 tatcgttcca gaacgcaaaa atcatgatgc cgaccaggag aaccagtccc acctgttgtg
   113281 ccagttctcg ctgccgatct tcaagtggtt ttctacgaat ggcctcaata aaaaagaaca
   113341 ggaggtgccc gccgtcgaga atggggatgg ggagaagatt cagcacgccc aggttgatgc
   113401 tgagcatcgc catcaggaac accaggctgg aggtgccctg ttcagcggcg tcccctgcgg
   113461 ttttggcgat cgtgagcggc ccgccgatgt tcttgcggga gatgtccccc gtgatgattt
   113521 tgtagatgcc caccaccgtc agttcggtcc atccccaggt ggcctgggcg cccagccacg
   113581 gggctttgag cggatcgttc gtctggagaa tagtttggtt ttgggccgaa atgccgattt
   113641 tcccgacttc ggccggcttt ccctctacgg tggccttttc ccccatcggc gtgacggaga
   113701 gcgtttgggt ggtatcgccc cgttgaatat caaattgaag cgcgcgattc gggctttccc
   113761 gcacgaggga ggtcatctgc gaccaggtga agatgtcgtg tccgtcgatg cggacgaccc
   113821 ggtcgccagc cgataaacct gcagcctgtg cgcgagaccc cgggatcacg gccgtgatga
   113881 ccggcgcccg ctcttcaatg ccaagctgga agatggtcgt ggctttgccg ttgtcctgca
   113941 cggtcgtttt gcttggtgtg accagcaccg tcttgacctg ttccccgcgc gtcaggtcca
   114001 acgtgagttg tttcccgttg ctttgagcga tgtatttcag gagttcggcg ttggtcgaga
   114061 tttctttttc gttcacccgg atgacccggt cgccaggctt caggccggct tggtcggcag
   114121 gtgatccagg gagtacggct tcgacttcag gaatgatgtc cttgaagctt gggaccggca
   114181 gcgtatagcc cagtccgata taggcggtgt aaatgagata ggcgaggata aagttgaaga
   114241 tcggaccggc cgcgacgatg agcgtcttgc cccacagcga ttgatggaca aacgcccgtt
   114301 tcttatcttc cggcgttaag gtttcgtgct cgtcttcgcc gaagagcttc acatatccgc
   114361 ccaggggcac gaccgacaac agatattctg tttccccgat ctggcgcccg aaaatctttg
   114421 gtccaaaccc gagcgaaaac ttcaggactt tcactccgac ccagcgcgca gcgagaaaat
   114481 gtcccatttc gtgaaaggcg acgaggacgc ccaacaccac caggaacgga agcgcccagt
   114541 gggtcagaag cgagaccaca tcaggagacc aggcaaaggc tgttcccaca acaacactcc
   114601 ttgggttcag ggtattaccg cgtcaaggca tgcacaaggg actcggcttt gtcacgcgcc
   114661 cagcgatcgg cttccagcgc atcatcgagg gcgtcgatcg gacgcggcgc atgagactcc
   114721 attgtactcc gaataatgtc cggaatatcg agaaaacgaa ttccgttctg aaggaatgct
   114781 tccacggcca cttcgttggc ggcattcatc gtcgccggca tggttccacc gatccgcaac
   114841 gcctcatacc cgagctgcaa acaggggaat cgctcatggt cgggcttgaa aaacgtcaag
   114901 gtgctgattt ccgtcaggtc gagagacgga aggtctaacg gcatccgttc aggataccgc
   114961 atggcataag aaatcggtgt tcgcatatcc ggaaggccca actgcgcaat caccgaccgg
   115021 tctgtgtatt ctaccagtga atggatgatg ctctcgcgat gcaccaggac ctcgatctgc
   115081 gtctccggaa tatcgaagag ccagcgcgcc tcaatgactt ccagcccttt gttcatcagg
   115141 gtggccgagt cgatcgtgat tttggagccc attttccagt tcgggtgctg cagggcgcgc
   115201 tcaggactga cgtcttgaag ttgctcgcgg gagaagcccc agagcgggcc tcccgaggcg
   115261 gtcagaatga tgcgcttgac gtcctcgcga cgatgccctt cgagcgactg gaagatggcg
   115321 ctgtgttcgc tgtcgatggg gaaaatgcgg acgtgatgtt tcttggcttc tgcctgcatc
   115381 aatgcgccgg ccatgaccat tggctcttta ttggcgagcg caatctgttt gccggcgcgg
   115441 atagccgcca gcgtcgggag cagccctgcg ccgccgacga tggcggaaat gaccagttca
   115501 gcttcctgcg actgcgccac ttcggccacg ccttcgttgc ccgcaaggat cttgacgggg
   115561 aggtcggcgc atcgatcacg caatcttgcc gccgccgcct cgttggcgag cgccgcgaag
   115621 gcaggacgga acttgcggat ctgcgcctcg agtttctcgt cgttcgagcc ggcggtcagg
   115681 ccgaccacgc gaaactcttg cggaaaccgg tccacgatat cgagcgtgtt cgttccgatc
   115741 gagccggtcg aaccaagaat gacgatgttt ttcatagcgt gctccagaac ggggggacaa
   115801 tcatctgtca cccgttgttg acgatcgtga catagtagta aaaggccggg ccggtgaaca
   115861 agaggctatc caggcgatcc agcatacctc catggcccgg gatcagggcc cccgagtctt
   115921 tgaatcccgc actgcgtttc atggccgatt ccgccaggtc gccgatgagg ccggtgatgg
   115981 tgaggatcat cccaagcgcc aggcaatcca ccagcgagaa agcgggtagg aaccaggcac
   116041 gcgcgacgac ggccatcagg caggcgagga cgaggccacc tgccaatccc tcgtaggttt
   116101 tcttcgggct gatgaccggc gccagcgcgt gccgtcccat ggactttccc gccacatagg
   116161 cgccggtatc accggcccac gtgaccagga agacgaagaa aatcagcagg gcgccgtcgg
   116221 gcaaccctct cgtgagcaca agatagctga gcgtgaggcc gagatagagc acgcccatga
   116281 ccagcaccat gccgtccgtg agtgagtcgc gcagcgactt tcctgagagc aggggcatgc
   116341 acagcgcaat cgccagggtc ccgagcagta ccgcacggtc ggtgacgagg gtcggccact
   116401 gcgcgctgct gatcaaaagt ccggtcgccc cgacgcccag ccagcaccac catggccaag
   116461 gggcctgtcc caaatggagc cgataaaact ctccggctgc gagcatgccg gcaatcgcgg
   116521 ccaggccgaa gaacgcgatc gcgccaaggt cgtggacgag gtaataaaag atcggcagaa
   116581 acaccgctgc cgccagtacg cgacgaacgg tttcggatga agatcgtggt cgagccctgt
   116641 ccagtgtggg cggcatagtc ttctcgcact gaatagctcc tggtttcacc acggagaagc
   116701 taggaggaga cgctgctgag cacccggccg aagcggcgtt cgcgccgctg gtattcgatc
   116761 agcgcgacca acgtttcgcg ccgccggaaa tcaggccaga gtgtcggggt gaagtacagt
   116821 tccgtatagg ccagttgcca caacaggaaa ttgctgatgc gcgtttcgcc gctggtgcgg
   116881 atcaggaggt ccggatcggg caggtgatgg gtgtacagag cctgttgcat ggcctgttca
   116941 tcgatctgat ccggtgaaag ccggccgctc tgcaccgccc gggcgagttc tctcgcggca
   117001 tcgaccagtt ccgcacggcc accatagctc aaggccacat tgaggatcag tttgtccaag
   117061 tgggcggttt cctgctccgt cgtccggacc cattgtaaag cggacggggg cagggccgtg
   117121 acccggccaa tggtctgaaa gcgcacgcct ttttcaacca ggctggatcg ttctgtggat
   117181 aaataatgtt ccagcaggcc catcaacgcg gtgatttcct gggcgggccg gttccaattt
   117241 tcctgcgaaa acgcataaat cgtgagcacc ttgatcccca gttccagaga aagagagatc
   117301 agttcccgca cggacttgat gccctcctgg tgcccggcaa tacgcgggag gccccgtagc
   117361 tcggcccagc ggccgttgcc gtccataatc acggccagat gtttcggcag caggtccggt
   117421 tcgagttgcg agagcagatc agagtcgggg gcaggctcga tatcgcgtga tcgagaatcg
   117481 ttcatggaac ggcgtgagca ccttttttta gactatgtgg attctcgggg aagtaaggcg
   117541 agtctagcgg tggggtccag gaaagtcaaa cagtatttcc caatttcgct gtatttgcag
   117601 atggttcgag cgtgtttgca ttgcgcctcc ggaaaagagt gcgttatact cacgcaccgt
   117661 acgagaggag cacatgcatg tccaatgacg agtccggcgc actggcgcgt ttcggggtaa
   117721 ccgatcgaga aatcgagcag gcgctgggac gggtcaaggt ctcgaccgtc gactatgcgg
   117781 acctgtattt tgagtattgc cagtccgagt cggtctccat ggaagagggg atcgtcaagc
   117841 gggccacgaa gagtgtcggg cagggcgtcg gcgtccgggc aaccgccggt gagaaaacag
   117901 gctttgccta ttccgacgaa ctgaccccca aagatttgaa cattgccgcc gatacggcgc
   117961 ggtatatcgc caatagcgcc cagggaacgg caccggttcc ggtcacccac cgccccgccc
   118021 tcgcacgcaa tctctatccc catgaccggg ccaatattga agtcgccacc cgcgatcggg
   118081 tcacgctgct caacgctatc gatgccgaag ccaggcggta cgatccccgg atcaagaacg
   118141 tcatggcggc ctataacacc gaatacaagg tcatgctggt ggcgacgtcc gacggtctga
   118201 tggtcggcga tatccaaccc ttgtcgaggc tccaggtcac ctgtattgcc gaagagaacg
   118261 gcaatcggca ggtggggacg ttcggcggcg gaggacgaat cggactggag tatttccaga
   118321 acgaccagcg atatctgcag ttcgccagag aagcggcgcg ggaagccatc ctgaacttga
   118381 gcgcagtcga tgcgccggcc ggtgtcatgc cggtggtgtt gggcggggga tggccgggga
   118441 tcttgctgca cgaagcgatc ggacatgggc tggaggccga ttttaaccgg aagaaaacct
   118501 ccgcgttctc gaatctgctg ggaaagaaag tcgcttcaga ggtctgcacg atcgtggacg
   118561 atgggaccct tcccttccgg cgcggctcgc tgaatgtgga cgacgagggc acgcccacca
   118621 gccgcacctt cttgatcgaa cgcggtattt tgcgcggata catcaccgac aaattgaatg
   118681 cccgcctgat gggtattccg gtaaccggta acgggcggcg tgagagttat caaagtgtcg
   118741 tgttgccgcg gatgacgaat accttcatgc tggccggaga atcagatccg aaggacatca
   118801 tcaagtcggt caaaaagggg ctgtacgccg tctcgttcgg cggcgggcag gtcgatatca
   118861 ccaacgggaa gttcgtgttc tccgccagcg aggcctacct tattgaggac gggcagatca
   118921 ccaagccggt gaaaggtgca accctgatcg gcagcggacc ggatattctc aagcaggtct
   118981 cgatggtggg gcacgatttg aagctcgacg aaggcatcgg aacctgcgga aaagaagggc
   119041 agtcggtgcc ggtcggcgtc ggcctgccga ccattcgaat tgacgaaatc accgtcggcg
   119101 ggacgaaagc ataaccatga cacgtgtgat cgcggaacag gactatacga atatcgcgca
   119161 ggatctgctg gcgcgtgcgg cgaaacatgg cgcgacggcg tcggacgtga tggtggccga
   119221 cggggaaacc ctgtcggtgc aggtccggat gggcgccgtc gatcgactca cgaaggcacg
   119281 tgaaaagcgg ctcggcctgc gggtcttttt cggtcaacgg tccgccagcg catcgacttc
   119341 cgacttttcc cgcgagtccc tggaacggtt cgtcggtgaa acctgtgcct tggcgcaagc
   119401 ggtcgtcgag gatccggtgt ccggccttcc ggagccgggc cagttcgcca ccgatttccc
   119461 tgagttgaat attcacgatt ccacgaaact tcagacggat cagcagatcg acctggcgct
   119521 gcgtgccgag cgggctgcgt ttgccgccga ttcgcgtatc acgaattccg aagggggcga
   119581 gtgcgactcg tcatccggcc ggatcatcct ggcaaacagc cacgggtttg tggggcacta
   119641 tgccaacagt agtttctccc tatccgtctc gccgattgcg tcggatgcgg ccgggatgca
   119701 gcgggattac tggtacgggg tgaatcgtgc gtttgccaaa ctggagagtc cggaagctat
   119761 cggccgggaa gcgacgaggc ggacggtccg gaagctcggt gcgcgcaaag tctctacctg
   119821 ccgtgtgccg gtgatctttg accctgaagt ggcggggagt ttactcagtc acctctgtag
   119881 tgccctctca ggctatgccc tgtataaagg cgcatccttc ctgatcggac aactgggcca
   119941 gcagatcgcg ccggactttg tcaccattta tgacgacgga cggatgcccg gcggcttggg
   120001 gacgcgtcca ttcgatggcg aggggctgcc gaccaggaag caggcgatgg tcgagcgtgg
   120061 tcgtctggcc agttatatcc tggacaccta ttcaggcaag aagttaggac tgccttccac
   120121 cggaaatgcc gcgcgaagca tcggagaaag tccctccgcc gggccgacga atttttatat
   120181 ggtcccgggc accaccagtc cggaggatat cttggcgtct gtgaagcaag gtctctatgt
   120241 gacggatctg atcgggttcg gcatcaatat ggttaccggc gactattccc gcggggcgag
   120301 cgggttctgg attgagaacg gcgagttggc ctacccggtg gaagaaatta ccatcgccgg
   120361 taatctgaag cagatgtatg cgaatatcga gacgatcggc acggatctcg tgtttcgcgg
   120421 acggattgcc agccctaccg tgaagcttgc ggagatgacg ctggcaggga actaggttgt
   120481 gagggggggg tacgtgatgc gacgactgat ggcctcgatg atctgtgcct tgctggtggg
   120541 tcctgcggtc gggagagcag cggaatttca ggtgacgagc ccgacgatca aggatcagag
   120601 cacgatcggc aatgagcacg tgtttaacgg gttcggctgc acgggtggca acctgtcgcc
   120661 tgaactgcga tgggatcatg cgccgaaaga cacgaagagt tttgctgtga cagtctatga
   120721 tccggatgcc cccacgggaa gcggttggtg gcactggctc atcttcaata tagctccgag
   120781 cgtgacgcac ttgccggccg gagcggggca gccggagagt gccggcgcgc ctcaagggag
   120841 cattcagagt atgacggatt ttggccagcc tggttttggc gggccctgtc caccgccggg
   120901 agataaaccg caccggtata tcttcacggt gttcgcgctg aaggtggatc agttgccgct
   120961 caagaaagag gcctccggcg cgatggtcgg gtactatctc aatcagaacg ccatcggaaa
   121021 agcatccttc accggcacct acggccggta agcgaacaat catcagaaca gagtaaggga
   121081 acagaccatg tctcttgatg ccgaactggg aaagacgatg cttcagttga tcacctcgcg
   121141 atacgaggat cggcactgga gaaaaaaaat cgaaaagaca ctcagtctgc cgcagagcgg
   121201 ggtcggagac gatcatcagc agcaggtctt catgtacctg aagctcaacc tcaaggcata
   121261 caagtccagg cgggcggatc cggattcctg gatcttaggc gggtacgcca cgaaggaagt
   121321 gattgatcgc gcgaagttcc agccgcacct ggtcggagaa ggtatttcag ccgacgatgt
   121381 ggcgtttctc ggcgtcgatc ccggccctga aatcgatgag gcctggtggg aagagatgtt
   121441 ggtcgcctgg ttcggcgttc cggaagaaga ggagtcgtcg gaggaagcca cggctgatga
   121501 ggccgcaact cccgatgccg tgaaggatcc cgcccgcacc tgaaggcggc tgtgacggcc
   121561 ccggacgggc tgtcgttcct gattcagtct cttcggagcg gtcttgctat gacccgaaca
   121621 aatccatttg ttgcgcgatc gtagcggggg gatcgatccg caacgtttcg gtggtgctcg
   121681 aagcggctcc ctcctgcgtc gcaggaggct gactgtgccg gtcgagaaac tccctcagct
   121741 tctgaaagtc ctcccgcaac ggctgcagca tggtcccctg gtgcagcgct gcggcgggat
   121801 ggagcagggg aaacagcacg aattccttca gataaaacgc ctgcccgcgt actctcgtaa
   121861 tcccgacctt gcgctccaga agtgtctgcg tcgcccaatt gcccagcgaa cagaccagct
   121921 tcggccgaat catggcgatc tgctgcaaga gaaacggttt gcaggtctcg acctcctgag
   121981 gttcggggtc gcggttattc ggcgggcggc atttgatcac attggcgatg tagatatccg
   122041 cgcgcgacag ccccgctgac tctaacaagt cgttgagcaa ttgtccggct gccccgacga
   122101 atggctcccc cttctgatcc tcgtgaaatc ccggtgcctc ccctacgaac atgacggatg
   122161 catgagggtt gccgacgccg aagacgacct ggcgtcgacc gagcttggcg agcgggcagc
   122221 gttggcaatt gtgaagcgac tcggcgagtt cctgcagggt ggtcagggat gaagacatat
   122281 gcgttccatt cgagtggagg gccaaatctt agtcgcggtg ggaggggttg tcaacgtgat
   122341 gtcgtgagcc attcgcccat cggacgagtc atgatgcgtc ggcgggaacg agcgcagcgg
   122401 agcgttggcc gctgagaatt gcggactcca gactggcagg ccagccggtg tccgtccagg
   122461 cgcctgcgac gagaaaattg gtaaacggac tgtgtgagag cggtcggcac tgttgcgtac
   122521 ccggtttggt ggtcaggatg gccgacggga gacgaacgac atccgactcg atgagcctgg
   122581 gaagcgggcc gccggggaac gctctctcca tgtctttcag ggtcagttga acgaggtcgt
   122641 ctttcgattg cggcaacagg tccggctcat cgactgcgac ggcccacaac acagcggcct
   122701 gctcgtgccg ttcctcgtct gcgtgccgga tcatccaatg aaaccggttg cgttccagaa
   122761 ggacgagttg tgtctgttcg accggttgcg caaggtgcag gcgaacgatt accaggggag
   122821 actcgctcag gcggctgatt tgctggaaat aggcatagtg cgtcacgact cgttccggca
   122881 agagcggcgt gagtcgatgg tgcggcagcg cggcgatata ccaatcggcg gtgagccggg
   122941 tgcggtctgc cagttcgaca tgggcgacac gatcctgggt gaagtgcaat tgtgtcgccg
   123001 tcgtgttcag ccgagaccgg actcccatcc ggtcaagctg cgcccgaagc ggcgtgagca
   123061 agaatgaatc gagtccgtga ggcgggatga tcaacttcgt ggcgcgggca ccggtcaaaa
   123121 agcagcgtcg cagtgtgcgc atgaaaagct ctgcggatac ctgcggaagc gccgctccca
   123181 gcaacaagcg ggcaaggctg ttccagacac cgtgccgggc atgttccgac tggccgatgc
   123241 tcgcgagcca ttcgtcggcc gctcgtgtgt cgagatcgtt cggcaacgga ggatcctgtt
   123301 cccatgtgcg ttcaaggaag gagaggagat gccagcggtc ccgcatcgac agaccctgga
   123361 acagggtggt tccgaggagg gtgttgagcg gggacggcag gggaaggtgc agaaattgga
   123421 tgcgagctcc cgtcgcctga agaaattcaa gcggcgtatg tcgaagccgc ctggtggcgg
   123481 tgtctttgcc gagggtcttg agcagggacc aggtggctgt gtgggcttcg aggagcaacg
   123541 ggggaggcgc gtggcagagg cggcctccca ggtggtcggc ctgctcgatc acggtgacgg
   123601 catggccgtc ggcggcgagg tgtaaggctg ccgtcagacc ggcgatcccg ccgccgagga
   123661 tgagcactgt ccggttcatg gagtcgaaga ggggagacgg gagcgcaacc acacgcctgc
   123721 tgccacggcc aaccgatggc tcggagtcag cgtgacccga tctcccaata cgcggtaacc
   123781 ggactgctcg atccggtgca agatccggct atacacaccg cgcatgattt ccgccaccgt
   123841 cagggcccgg cgttcggcgc gtggcagcga atcgagcgcc cgcgccgcct tggcgtaaaa
   123901 ttcctttgct cggccgactt caaaccgcat gaactcggta aattccggtt tgtaccgccg
   123961 gtgcaggaga tcctcctccc ggtaggcgaa acgcgcgagg tcttcttgcg gcacatagac
   124021 gcgaccgcat tctgcatcgt tgcccaggtc gcgaagaata ttggtcaatt gaaaggccat
   124081 gccgagattg acggcatagt cctgggcgcg cggggaggtg gtgccgaaca cgtgcaggca
   124141 gatcaatccc accaccgacg ccacccgata acagtagaga gacagttggt cgaaggtggc
   124201 gtaccgggtg gtcgtgaggt ccatctcgac gcccttgatg agttcctcga aataggcctg
   124261 cggaatggac agctcccgta cgtgccgggc aaggctgacg gtgacgggga acgtcggcgt
   124321 gccgtcgtag gcggcggcca gctcgcgccg ccagcgggcc aattcttcct gcggatgact
   124381 gccgggaggc ggctcgtcca cggcgttgtc cacttcctta cagaaggcgt agaccgtgta
   124441 catcgcttcg cggcgggctt tcggaagaaa caaaaaggag tagtagaaat tgctcccgct
   124501 ttttttggtg agggtcgtgc agtaggtttg ggcttcagct ggtgtcatca tcgtgtcatc
   124561 agtgtgagtg cgtcgtctgt ccctcaagtc gttcgacgga tgcgggaagg tggcggccgt
   124621 aagaggctgg gtgcaacatg gcggcgagtt gttccacgcc gtcgatcagt cgtgggccgg
   124681 ggcggctgaa gtatgagagc gcatcgacca ggtagacttc tcccctctga acggcgggaa
   124741 gatgttgcca ctgcgggtgc gccgtcatgc ttgcgagttc ggctttcgtg cgcgggacgg
   124801 taaatccgca gggcatgagg acgatcacct ccggttcaga ggccgtgagt gtgtcccaat
   124861 ccatttttcg ggaggccgtg cctgcttggg ccagaacgtc gatgccccct gcagcggcca
   124921 ccatgtcggg aacccaatgg cctgcggtgt agagcggaga gagccattcg atacaggcga
   124981 ctctggggcg cgttgcctga gcgcccacgg tggcccgtac cgtgtcgagc ctgctgcgga
   125041 gcaccgcgga caactgtgtt ccggcttctt cccgcccgat ggctgttcca atcgtttcaa
   125101 tatccaggag gatgtcatca agtcgcccgg gactgagggt gagcaggcga ggcggcggca
   125161 agagtgcatg aacgacccgc tccaactgtg acggggtgat ggcgcagaca tcacacagcg
   125221 cctgggcaat gatcaggtcc ggtcctgcag ccaagaaccg tgtctcatcc aattcataga
   125281 gacctgcctc ggcggtgagc agcgcgccga cctgctggtc gatctcagcg ctggagagat
   125341 gatggctctc gatgcgcgga cgcaccatca ccgggatctg tgcgaggctc ggcggaaagt
   125401 cacattcatg gctgatcccg atcagatcgt gttggcaccc caaggccgcg acgacttccg
   125461 tggcacccgg caccagcgaa caaattttca tgggcggcct tgagccgctc cccacaggcg
   125521 cgcctgcagg tcctgcagtc tggtgtctgc aagcgaatgg gtaatggcat cggcctctcc
   125581 gagatcctgc accgtgtggg tattggcgac agccagcact ttcatgccgg ctgcgcgcgc
   125641 ggctctgatg ccgggcaggg aatcttcgat gaccaggcag tcgttcgggg acagtgcggg
   125701 ttggttcggt tgtgagttca agccggccat cgcgtggagg aagggatcgg gcgccggctt
   125761 gccacgggtc acgtcttcgg cgctggtaat gtgcagaaat gccttacgaa gacccgcctc
   125821 ttccagaatc aattcgattt cattccgcag ggcgccggag gcgatggcga gcggatatcg
   125881 agcggcggcc tcatgaatca gttcgcgcac gccggggaag attgccagat gttgtttgac
   125941 ggcggcgagg taagcgtggg ccttgtgctc catcaattcg ccaaggagcg tgggagacgc
   126001 ctggcgccgg tgggactgca aggccgccag gaagcagccg cgatcgtcga acccgaggta
   126061 gtcggtatag tactccgctt cggtgaggga gatctcaatg ccggccagga cctgtcgcag
   126121 ggcggcaaag tgcaggggct cggtgtcggc aatgactccg tcaaaatcaa aaatgatggc
   126181 gcgtagttcg ctcatggtcg ttgttcgggc atccctgtgc gtagttcatt gaatatcggg
   126241 ctgtcggtcg tgtgggcgcg gcattataac aagggcgtac tgacaagaaa agacgacggc
   126301 ccgtcccact gaggaacggg ccgtcatacg aggcctggga gacgatcgtg ggcttagcgt
   126361 ttcagcctga ggctacgttc agggacccag ccttttcgac catcctgagc tcgaaccgag
   126421 tagacccagc cgcgcttttg gtaatcgccg tccaggatcg tgagcgtggc gccggccagc
   126481 actgtcgtgc tggttttagt ccctacagcg tcaacgaaga ggggtatggg ggacttgggt
   126541 ctggtcggat cggcgatgac cgtcacccgt tgaccttccg catagagcgg accggcgacg
   126601 cgcacggtcg gagccgcggg cttgggggct gaagctgcgg gctggagggc cggcatggca
   126661 ggtgcgggta ttgcgggagc cgacatggcg gacgacggag caggcacatc agccggagcc
   126721 ggggtgctgt tgtcggccac cacgggtggt gccaccgtcg gtgcttgttt cggagcttga
   126781 gctttgaccg gcggtttctg gatcacgggc tgagcgggct ctgcgccgcc acccagatag
   126841 ggttcaacca gctgcatggc tccatccggg tccatggcga cgtagccgac tcctcccacc
   126901 agcagaagca gcagaatcca gagcaacggc tttttgccgg atcgcttcgg cgagttcatc
   126961 ggtggcgccg ggggcggcat tgattcatcc aggtcttcct cggtgaattc cagatccggc
   127021 tccggttgac ttgcgaacag gagatgccag ttcgtccact cgctgcgcat ggcaggaatg
   127081 cccgtagatg aaaccatcgg aggacctcct cattcgaaac aatggcgcgg gtctgtctat
   127141 ttgtaggtac ttatcaccgg ccccacagcc tgtcaatttt gttcgacaaa gcgacaggcg
   127201 gcacgcatgt tctgcgacaa ggcccggccg gccgggagtc tgtccgacat tgaccgttct
   127261 actgcggcaa acgatccacg atcatctgag tcgttctcgg cccgaaatag tcctcaacgt
   127321 attccagcga atacgcttcc gatttttccg ggcctgcggc ctagcccgga ttattcatga
   127381 atgccgttgc gaggcgtttg agaccgacgc ccgccggggc ttctcgcaag taaggccgac
   127441 gcaggcgtac gtgcagtccg tcgaggaagc cgaacgagaa cagccccgcc gggcgagagg
   127501 atcggacgac ggagcaggca ttcatgaata gtccgggcta gcatctggtc gcgcctcctt
   127561 cgcctcgcta cgaaggccat gtcggacagg ctcctgggtt ggaaatgtcg aagcgattgc
   127621 tgccgcggag gcggatcgcc tgcagggttg acttgatgcg caagggcttg caagttccaa
   127681 catcctcgtt gcagaatcgc gcaccaccct gaatgtgaag accgatgaag tcctatcgag
   127741 aagaactctg gtttgaaacg aaaaccaggc gcgcatatgt gaatatcacg ccggaggtcg
   127801 atgccgtggt gcgaaaaagc ggggtgcggg aggggatggt gctggtgaat gccatgcaca
   127861 ttaccgccag cgtgtatatc aacgatgacg aggcggggct gctgcgcgac tatgatgagt
   127921 ttcttgagcg tctcgcgccg cacgaggcga cgtaccgcca caacgacacc ggtgaagaca
   127981 acggcgatgc gcacatcaag cggcagctca tggggcggga agtggtggtc gccattacgg
   128041 atgggaggct ggacttcggg ccgtgggaac aaatatttta cggagagttt gatgggcggc
   128101 ggcgtaagcg tgtgctggtc aaggtcatcg gagaatgatg cgtcgaacgt tctgtggcga
   128161 ggctgccacg gatggtgtgc cggtgaggtc gacgaagaac cgcattcgcg cttgccacgc
   128221 tctggaaagt ttggcaacat ggataacagc atgaacagat accattcagc aggacttcgt
   128281 atcgacatgg gaggccagcc atgttgagtt ggtcacgacc ggatccgctg acgagggatt
   128341 tgattcattt gatcaacgcc cgtcgccatg accatgggga cacggatgcc gcaatcgata
   128401 ccctgcaagc ctttatcgag cagggacgtg agcaggatct gctgaccgtc ttggcggcgc
   128461 tcgatgagga gatggcggag tggatgtttg atctggtggc ggaggcctcg tgcgccgtca
   128521 tcatggatgg gccggatgac gacaagggcg cctatgcctt gatggccgcg ttggagttgc
   128581 cgctccaagc caacttgggg cagccgcttg gattgaagat cgaccccgtc gcgacggcag
   128641 atctgctgca tcgacatttg cagattccgg ccggcgtggt cgtgcgggtg gaaccccgtc
   128701 tcctgaccga cgcgaccctc gaaggattga ccctccagac gttgaacgaa catctcgtga
   128761 gcatcgcgga ttctcagcgc actcaggcga ttgcgtcgcg tctctatccc ccggtcgaaa
   128821 acaccgcgtt tctgttcttt caattgatcg gcatgcccga tccgggcttg cccgattccc
   128881 tggaggaacc ggagcgccgg gcgctgctgg aaggtctcgt cgcacaatgc gccgagctgg
   128941 cgctggcccc ggacaccctg gaggtgccgg tctacgcctc ttatgcgctg ttcgacgcgc
   129001 agaatgcggt gcgactgcac cggttggccg acgaaaccgc cgcagtctgg cgggatttgg
   129061 atcccatggt gcgggcgcgc acgatcgccc atctgcacac gcaatgtggc aacggcgtgt
   129121 atatgccggt ctggaccgat ctcttcgatc cggagttgcc ggaggaccac ggtcctgtgt
   129181 ggacgtcgcc cgacgtatgg gtgttcacgg ccgacctccc gatcgaatgg ccgggggaaa
   129241 tgctgaccaa tcgattgctg gcggaaggtt gcacccgttt cgagaaccac atcgtcgaag
   129301 cgcctgagaa ctgagcggcg gttgccggac aattccatgt gtgccgcatc gtcgcccgcg
   129361 tcgaacgggc aatgggaagc ctcttcccag atcctgctga tcaggcagga tcgatctcat
   129421 accccgtagg agctccttgg ctggcccatc ccgactcggt tccatcgcga tcgtgacagt
   129481 actcagttgt gtggtgttgg gcgcggtctg gggcctggtg ctctatacgc tgccgcagag
   129541 cgacctggcc cccgaaccga caccggtttc cttcccgccg caacctgcga cacctgcttc
   129601 tccggcacat accggtgttc ccgccccgcc gaccccgggg ccggtttcgc aaccggtggt
   129661 gcgggagccg gcgccggtca tacaaattga agcccaggag catcctccgg ttgagcccgt
   129721 gcgtgcgccg ttcgggaggg agttgaaatg cgacctggaa attgaggcgc tgtgtcccga
   129781 ggaggaaggg gagcggcggg cctgtctgca acgcaaggcg gcacaactct ccaccccatg
   129841 ccggccgatg ctgcgggaac ggctcgtgcg gatgaaagag gcgatgcaac agctgcgtat
   129901 agcctgtgag gcggaccgaa ggcagttctg tcgggaggtg ccgctaggcg gcggggcgtt
   129961 ggtgcagtgt ctggaatctc atgcccagga agtctcggac cagtgttttc aactcctgcc
   130021 gaaacggggt cgcttgctca actagcccgc attattcacg atgactccta ctgcaaccac
   130081 cgacacgccg ctgtgacaag cttggctgcg agatgctcgc acccaagcgg gcgggctcgc
   130141 cccgagaggc tccacgcgcc cgtctcgcaa ggcgtctccg tggtttcgtg acggactctc
   130201 gtgaataatg cgggctaacc cgcgacgaga ctccccttcg ggtcgcgcag caaaaatcgc
   130261 atggtgaggc cggccacttc caccacatct ccgttgcgaa gcgcgtgttc cccctgaatg
   130321 tgacggccgt tcacgaggat acgcttcccg ctctcggtct ggctcaccac gtatccctcg
   130381 ccgcgccgat tgatcaccgc ggccgttttc ggcgcaaacc acccggtcaa tgtgatgacg
   130441 gcgtcgtcct gtgcgccgat cagcgacacg tgtttcgtca ggtgatactg tgattgcccg
   130501 ctctttcccg agagaatttc caccacgccg attttctgct ccgtcggcgg gcgctcgccg
   130561 cccatcggac gcgacaccac catcgtgtcg tcggtgatca ggatctcgcc agtgccggcc
   130621 gtttccgctg tgatcgggtg tgcctcccga aaaatcaacc ggtgcgtgcc gagacgaatg
   130681 ctgtctgcgt cgcgcagttg tcgccggtcg attttctgct cgttcacgaa tgtgccgttg
   130741 gtgctggtca gatcttcgag aaacaggact tgctgcacct ggacgatccg cgcatggtgg
   130801 gcggagacag cgaggtcttc caggcagaga tcgttgtcgt gcttccgccc gatcgtgaag
   130861 ggcgtgcgcg agacctcgag ttctcgtgtg ccgccctgcg gcgccttcac gaggagcgtg
   130921 ggtgatgctg ggtggtgctg ggacatggtc gctccttagg atgattcggt ggtcggtgcg
   130981 gcgaacaact gtgtgcacat gcgatgccac atgcccggct tgcggtgcct gagcatcgcc
   131041 acgatgacgg tcgtattgtc ggtgcccccg gcggcattcg acagggcgat gagacggtcg
   131101 gagacggtct gcggatcgga agactcctgc acgacgcgga ggatcgtgtc ggccgacacg
   131161 ccggcggtca ggccgtcgga gcacaggagg aacagatcgc cgttcaacac ggggagttct
   131221 cccagttcga cctccaccag aggctgcacg ccgacggcgc gggtcagcac atgcttgtgc
   131281 ggcgcgcgcg tcgcgtcatg ggcggtcagt agtccggact gcacctgctc ctggaccaga
   131341 gaatggtctg ccgtcaggag ttgcatcgca ttgccgcgga tcaaatagag tcggctgtcg
   131401 cccacgtggg cgatggagag aatcggcccg accagccagg ccgccacgac cgtggtgccc
   131461 atgccggcgt attcgacatg acgcgccgcc tcctgatgca ccttgtgatt ggccagccgg
   131521 acggcgctcg ccagccggtt ggtggctgat gagaactccg gtcgtacggc gccgatcagg
   131581 gggagagacg ggtcggtctc ggcctccgcc aggtggcggg gaatgatgtc gatgacgcgg
   131641 ctgctggcga cttctcctgc gcggtggccg cccattccgt cacagaccac gaacagaccg
   131701 gcggagggat cggcatgaaa gcggtcttcg ttgtgcgcgc gggtcaaacc gacgtccgag
   131761 gtcgctccat gtgtgacgtg caaggagtgc atggtcgggc ctatctcggc atcacttgga
   131821 agacgtcacg gaggtcctgc gccatatcgc tggcgtgtcg gtatcggttc gggatttctt
   131881 tctggagtgc gcgatcgagg atggcctgga cccgtgtggg aagatcgcgg cggtatttga
   131941 gcagcggggc atgcggagct ttggcgatgc gcgtgacgag cgccggcatg ttttccgcgt
   132001 cgaacggccg ttcaccggtc aacaattcga agagcacgat gccgagggaa aaaacatcgg
   132061 accggctgtc cacatctttt cccatggcct gctccggcga catgtatcgc ggtgtgccca
   132121 gaatcatgct ggtttgggtc ttcgagccgc tggccatctt ggcaatgccg aaatccatca
   132181 ccttcaccag gcgttgtttc aggatcatga tgttcggggg cttcacatcg cgatggatga
   132241 ccccttggct gtgcgcatag tccagggcat cggcgaccgt cgccacgatc tgcaaggcct
   132301 gcttggccgg aaggagggtg gatttctggc aatagcagct gagcaaggtg ccctcgaggt
   132361 actccatcgc gatataggcg agaccctcct gctcaccggc gtcatagacc gtgacgatgt
   132421 tggggtgtga gagccgtccg gtcgattcgg cttcgcgaaa gaagcggtcc cggaattctt
   132481 tgagctcttc ctcattgtcg atgtcgtcca gccgcatggt tttgatggcg acgtggcgtt
   132541 ggatcgtcgg atctttgccc aggtagacga cgcccatggc gccacgaccg agttccctga
   132601 cgacctgata gcggcccagc gagtccggaa cggcggcccc attgcggacg atcacggtgc
   132661 cgcctgcacg ggccgggttg ggagtaaccg cgtcggcggt cgcgcgggcc tcgccggccg
   132721 tgtctgcctg tgcgtcttcc tcctcaaccg gacgacggcg tggacgaccg acgaaggccc
   132781 gggtcagcgc gtcggcatga cgaacccagg cgaacgtcag ccagatggcc gcgccgatca
   132841 ggccgccggt gatcagccag ttgtagagcg gttgcttggg agtcccgagc accggaacgc
   132901 catgcgcctc atatgcgcga gtggccacga ggacgatcag gaggagcgcg gagggaaaca
   132961 gaatcgcacg gaaaaatgtt tggcccttgc cgttgtcgtg caggtcctgc ctggccctgg
   133021 ccgcggtgag ccagatcatc aacaggcaga tcccgtcggc gatcaatcgc acggcctgcg
   133081 cggcgttcat gcgtaacggt tggaggaacg tgtgtgtgaa cagcggaagg gtggccagta
   133141 acggcccgac cataatcgca aggataatga cgatcaaata cgggccgatc cagccccagg
   133201 atgtggtcat gtgttggagt cgtgcgtagt tatccctcag tgtaaccgaa ggtcttggag
   133261 tgtcaacgaa acgagggaat agtgcaccct agcccggatt attcatgaat gccgtcgcga
   133321 ggcgtttgag accgaagccc gccggggctt ctcgcacgta aggccgacgc aggcgtacgt
   133381 gcagtccgtc gaggaggccg agcgagaaca gccccgccgg gcgagaggat cggacgacgg
   133441 agcgggtgtt catgaatagt ccgggctaga gacaggggag ggattggtcc cggagagggc
   133501 gatgcggtga tcgtcgggct acttgcctga agatcagttc gaggttgcgg cttttctgag
   133561 cggatcgagc agtttctcgg acaccttcaa ctgaccccgg ccgatgccga cctcgatatc
   133621 cggcttggtg acgccgtgga aatcgcttcc cccggtgacg aggaggtcgc actgcttggc
   133681 cagctcaagg tactcggtgg tttggctggg tgtgtgagtg ctgtagtgca cttcgatgcc
   133741 tcccagtccg gcggctttca ggtcgcggac cagggtgcgc aagccgtctg cggatgttct
   133801 cacccaggtg ggatgcgcca gcaccggcac gccgccggcc tcacgaatcc atcgcaccgc
   133861 ctcggccggt tccggcaact cacgatcgac aaaggccggg cggccgttcg ccagatagcg
   133921 gtcgaaggcc tccttggctg aagtgacgaa tttcttctcc atcaacagcc gggcaatgtg
   133981 ggggcgaccc accgattcgg tgccggccag ggcccgcact tcttcatagg taatcgggat
   134041 gcccagctcg ttgaggcgtt ggacgatctt cggattgcgt gtgtggcggc tgtcccgcaa
   134101 agtgctgaga cgttgggcca ggagcggatc ggtccagttc agaaaatagc cgaggatatg
   134161 gagttcgctc tcgccgaggc gcgaactgat ctcgacgcca gggacgactt cgatgccgag
   134221 ttcggccccg atggccgtgg cttccgcaat cccctcgaca atgtcgtggt cggtgatcgc
   134281 gagcgcggtt acgccggcct gcttggcaaa ggccattacg tcgcgggtgg aaaagctgcc
   134341 gtccgaatgg gtggtatgga ggtgaaggtc gatgcgactc atcgtggtag ttcaggcccc
   134401 gctttctgcg cgaccaagcg cgcggtgtag catggcccgc tgccatggcc cccgtcatgt
   134461 tcgccctcgt cgtaatggag cacgcgaagc ggggcggtca atcggagtag ttcgttctgc
   134521 gccagacaga attcccatcg gcgcggatgt tggtgacgga agtaattctc gatgctgaag
   134581 gtttcgtaga tcaagatgcc gttcggtttc agcgcgtcca ccagcacggg gaagagggga
   134641 cgatgcaggt aaaaaaagac gacgatggca tcataacatt cccggcccag gtcgggggga
   134701 tggtcgggct tctcttccaa atccagcacc cgcgttgtca ggcctgagag ctgccgctcc
   134761 tcggcggcct ttttcagcgc cgtcagtgcg agagcgtctc gatcgatggc ttcgacttgc
   134821 atgccgtggg acagcagatg cagggcatgg cgtcctcggc ctgccgcgac atccaggacc
   134881 cggcctttgg ggaggcgccc aagctgctgc gtcagaaacg gcgaggggct cggtcctgcc
   134941 tgctgcgcgg tgcgagtccg gtccacacga acaccgactg agccggcgac catcgccaac
   135001 ggcgcatgga gttttcggat ccaaagctgc actcggtcga ccgggaattc tgcgaagagc
   135061 atcccgacca gctgttccgc gagggtttcc aggaggcggc aggtcaacga accgttgagc
   135121 gccacgaggc gttccgcgac ccgggcataa tcgatcgtct catcgaggcg atccgagagg
   135181 ccggcggcgt cgaccgccgc ttccaactcc acatcgatgg cgatcaactg cggtttgccg
   135241 cgttcctctt ccgtgacgcc acagcggccg taacactcca gacgttcgat gatgatgcgc
   135301 ggtgccatgg cggcattgtc gtggatgggc tttcgggctg tcaagcaagg tggggcggac
   135361 ccctgccggg cagggtgagc gtgaagcgtg aaacgtcaaa cgtacctccg aggaggccga
   135421 acgagaaaag cctcgacggg cttccgtctc gaacgcctcg cgacggaact catgaataat
   135481 ccgggctaga ggcgggcggc gagacgtttg gcgagtccat tcgccggcga gagcgcacgg
   135541 cgaaccgagg acaggatcgt tgagggtata cggatgttcg cgatgtgctg cagaaggcga
   135601 gcggcggtca atcgcagcaa ctctgcggct tgtgcgacgg tcggttcatc gccgcgacca
   135661 aggcgcagca gtttcaactc ggctttatac acagcgcggc gccgccaggt ggcatgaatg
   135721 gcgaccgcgg ccacgatggt gaggagcaag ggattcgagg ggatggagaa caaccatcgc
   135781 caccattcat agagggccag agcgccgaga cacatcggca gcgagaggac atcgccccat
   135841 gccgactgca ggcgccggat ctgttcgtcc acggattgcc cggtgagccg ccgtggagtc
   135901 agttgcaggg gagtgcgacg gtttttcgaa ccgaacaatc gcgcgggcag aaacatgcca
   135961 gatccctcct gtcgtgaacc agctcacgta cagcaggcat tgtatggcag gttggctgat
   136021 cggaatagga aagacagaat ttctgcagcc gtcccggtcg ttgttcccgg gcgtgcttat
   136081 cggtttgtct ctccgttaag tttgagcacc aggtaaggcg tgaggtaata acacaagacc
   136141 gaaacgatca cgcccgccgc cgcgccgatc aacccgcgca caaagacccc gaagaacggg
   136201 aggaggaaga cgaggaggat catcaccacc gcgatgggtt tacgccgctc atagaaggcg
   136261 gtgcgtcgcg ccaaggtgag cgagtcggag aaagacacgt gagatgggtc tgccatggga
   136321 ttgcacctat tccattttgt ccggattgcg tgtatgaacg tggagcatcg acatcgacag
   136381 ggtactagca ggctacggaa aaactcgatt gttgcccaat acgagacgat ccactcctgc
   136441 gccgtgtcgg tatggcagta acacgcagga tgcgcaaaaa ggccgtccgg caaggccgcc
   136501 gcaagcgaag aggcgaatcg tactcgcgcc gtacggtgag cctctgaggt tcacgacgcg
   136561 cggaataacg cgcgtcacgt ttgtgaacgc cgccgagatg gtgaggcggc agtgtcttgc
   136621 gagaacgccg ctggcgaact ttttccgcat cctgctactc attcatttac cacgggaggt
   136681 taggtatggc gaagggcatg cagcagaaaa aggcgacgaa gaagaagccg gcgaagacga
   136741 tgatggagaa acgcgctgaa aaaagtgcaa agaagggccg ctgattcaat ttcgtgcgtg
   136801 cgtcaccgcc gcccgacgat ggcgaccctc acctgtgtga ccggaagggc atggtatacg
   136861 gcgcccttcc ggtaaggcag tcaggttgac tctgctccgg tgcacccctc gtgctttccg
   136921 cctcaatgca acttctgcag gaccccctcc gcgacaacgg cgtccggatc gcctcccacc
   136981 atcagatcct tgtcgccgaa gtgggatgtt gaactggtgg tgacccgcat cttcaggccg
   137041 ccggggacga gggcatattc gaacagcgcc gcttcctgcc gctgatccag atagccttgg
   137101 ctcgcccgct cgaaggagcc gacgatcccg accgataatc gcccctgccc ctgctcgaca
   137161 cgcttcacga ccatccgttg cacgccgcct cccggcggaa cgtattccac cgtttgaccg
   137221 acctgcgaca gctccacttg ccgcgtcagc cccttgttgt tcgtgtacga atagagggga
   137281 aagccctgcg gactgaagtc gatttgcact tcggcctggc tgccgtcggc actgctgagc
   137341 agccctttcc aggcgccggt caggtctgcc gcttcaatgg cgtcggacag gaccagcgcc
   137401 atgaccatca ctcccatgcc gacaagccgc ttcatcttca tgaccctccc ctcctgtcgg
   137461 tcgttgtcgt cacaccgtga aagggcgctg gcgcacaaaa cgggcggccg ctccgagcgt
   137521 aaagagattc ggtgtctggc tcgccggcaa ttccgccctg atggccgcgt ggaacttggc
   137581 gtagttcccg ctgtagttcc catggttcca gaccttcagc aactgttcgg tgaacgctcc
   137641 gttctgctcg ccatccatgg aggtctggtt atcctggcat cccgagatga ggatgacgga
   137701 tgctttgaaa tctttcgcga tcgcggtcaa acgcgggctc acggctaatt gggcgagcac
   137761 gctatccggg tctgccgcag aggccttgcc ggccgctttc gcgacgttct tctgcaagct
   137821 gtcgtagaac tgctggtgct cgcgataggt gcggaggccg acggagatcg gcatcatttt
   137881 cgagcgcctg accggtgcgg caggatcgac ctggggtggc ctgttgcggg tgacggtgcc
   137941 actgtggcag ctgtcggaga acaccagaat gcgcacgccc gccgcgaagc ggctcaattc
   138001 gaagtacaac tcatcgtcga tcagctggcc gtcatacagg caccaggttt cgtcctgctt
   138061 gtccgcttct tcacccgtga cgtcggggac ttgtccgccg tggcctgaat aggtcagaaa
   138121 aaagaaatcg cctttccgca gtgccttggc ggccttgcgc atggccgcca gggtcttggc
   138181 gcgtgttcct tttttggtca gcagcacggt ggatttcatg ccgcgggatt tggcgatcgc
   138241 cgccatgtcc ttcgcatcga actcacaggc cgccaggtcg ccgctccagc caccgtaggc
   138301 ggcggggctc acggcgttca ggccgatgtg cagggatagt cccttcgatt tactgctgga
   138361 tgacgacgtg gtcgttgcca tacgtacctc cttggtcggc gggacagggt cagaatgaac
   138421 aagacgatgc caaaaatatg gcggtacggt aatccacaac ccacattatt catgagcgct
   138481 tctactgtaa ccgccgatcc gctgctgcga cacgccttcg gccggcgggc tcgcccctcg
   138541 gaccctccac gtactgcacg agtacgcgtc ggggcctccg gactccgcgc gccggtctcg
   138601 cgacgcgcct cagcgatttc gtgacgaacc gtcatgaata tgcgggttac ggcgaaatct
   138661 gtcaacggag agcaaggggc gaatggtggt atttcaaccg cgtcggggat gggaaagtcg
   138721 tgtcgtaaga aaaaaacgat gtcgccacaa actgtgaccc cggatgtcac accacggtgg
   138781 tagggtcggg cgaatgcgca gagcacatgg cgtatggcat agggcggatt gtaagagcga
   138841 ataggccagg ccacgtttct cgcgacgctt cgttcgggtc tcccgggaat gttcaatcga
   138901 gcatgtgtct tcaagacgag agacgcgtca tgaggcacgc ttcacgaagc agaggcccta
   138961 ggcatttacc tggttcgaaa cggcgtattt ctcgcgggac ggcgttgtcg attcttggta
   139021 cagtgcgcgg cattcggagg acgccatgag ctggaatgaa gaagcccgga tcggttgtcg
   139081 tttccacttc aagtgtcctc agctctggtc ccgcctacaa cccaccccag ctgaaagcgt
   139141 tcgacactgc ccggaatgtg atcgagatgt ccacctggcg ttgacggaag aagacttttg
   139201 tcggcacagc gaggaaggct ggtgcatcgc cgtaccagta ctgcggtcag aaaggcaggt
   139261 cgatcccgat ggtccgcact atgtcgttgg cagggcagat ccgctatatg ggcaagagtc
   139321 gtagtcacgg cagtaacgag gagaagcggc atgtttccag ttgcctatag aggcaatgag
   139381 ggcaagtgaa aagcctgaaa gggagagccg aatgagcgaa gtcaatcagt ttgcaaactg
   139441 gttcgcacgt gccacgggaa atgaacccta tccatttcaa atccggtttg cctgtgagcc
   139501 aaaactatca gagctcgtgg atgtgccgac cggtatgggg aagactgcga tggcggtgtt
   139561 ggggtggctt tggaggagac gcttcgctgg agagagagtc aggaaggcaa ccccgcgtcg
   139621 cttggtttac tgcttgccga tgagagtact ggtggagcaa acggttgaga atgctcaaga
   139681 ttggctcaag aacctagagc cccttggaga acttgagaaa ggtaaagtct ccgtgcacgt
   139741 cctcatgggc ggtgacatag acaacgactg ggatgcacac ccagaggtag atgcaatttt
   139801 gattggcacc caagatcaac tactttcgcg cgccttgaac agaggctatg ccatgagtcg
   139861 ttatcgctgg cccatgcact atgccttact gaacaacgac tgcttgtgga ttatggacga
   139921 ggttcagttg atgggagttg gggtgacaac aacggctcaa ctacaagctt ttcgggagaa
   139981 gttcggcaca tttggcccca cacaatccat atggatgtct gccaccttaa agacagccac
   140041 cttgggtacc gtggattttg cagaaagagc agaaacacta ccgcgtctgt ctttgaattc
   140101 cgatgatatc aagcactcac atgtcgctaa tcgtgtaaat gccgtgaagc tgttggagaa
   140161 agccgaaacg gcgtggaggc aagagacaaa agatagctat gcagatcagt tagccgagga
   140221 agtatgccgc catcatcagc cgggtactct gactgttgtg atggtcaatc gagtaggccg
   140281 cgcgcaagat atgtttgttg cgttaaagcg acaagtgagg agtgagccta gccagcccga
   140341 tctctgtttg attcattcca ggttccgcca ggcggaacga aagcgtctcc aacaacagct
   140401 aacagagagt cccggccagg ggcgaattgt ggtggcgact caggcgattg aagctggggt
   140461 ggatgtctct gcacgaactt tgtttgccga gctggcccct tggtcatcat tggtgcaacg
   140521 gtttgggcgt tgcaatcggt atggggagtg ggaacagaaa agttcggccc gcgtattctg
   140581 gatcgacctg caaacctcga ccacaaacaa gaagggtgag cagcagctag ttcctgaagc
   140641 ggctctgcct tacgagattg acgatctgga ttggtcgaga gaccagttga atcacctcaa
   140701 agcagtgggg ccaaacaagg tgtcgacgat tgcggctccc gaaaaagacg agttcgggca
   140761 tgtggtccgc cgccgtgacg tggtggatct atttgacacg acgccagacc tggctggaaa
   140821 cgacattgat gtctctcgat ttattcgcga cggagagaat aacgatgtgc aggtatattg
   140881 gcggcagtgg gacggggaag ctccgtcagc tgatatggct gcgccaagac cggaagagct
   140941 ttgcgcggtg gcaattggac gaatcaaaga gtttctaaga aaagatatcg tggcttttcg
   141001 atgggatggg ttggagcagc attggcttcc agtgaaaaaa gataatgtct ggccaggcct
   141061 gaccttactg ctccatgttt gcgaaggcgg gtatgcggaa gaccttggct ggacaggaaa
   141121 gaatgatggt gcggtgctag ccgtgccgtt gtctcctgaa ttgccatcca acgaggcact
   141181 tgatgaagac cgctttacct ttattaatcg gtttgttcat ttaacgaagc atgcaacgga
   141241 tgtagctgaa gagatgaacg cgttgaaaac agccctcgac gaccccactt tcaaagttcc
   141301 gtggagcgaa ttgctcacgg cagctcgatg gcacgacgta gggaaagccc atgaggtgtt
   141361 tcagaggatg ttgaccgacc cattactgcc taacaaccct aataagtccg gcggcccctg
   141421 ggcgaagtcc gatcataaga aaggccgccc ggaccgcaag cattttcgcc atgaactggc
   141481 ttcagcattg gccctcttgc tgcacaagga atctgacctg acggcctacc tggctgcttc
   141541 acaccacggg aaggtccgcc tctcaatccg ctccttgccc ggtgagcagg tgccgtcgga
   141601 tgcgcgacga tttgcgcgtg gcgtctggga aggagataag cttcctggaa ttgaccttgg
   141661 agatggaatt acaactcagg cagtgtctct cactttgagt ttcatggaga tgggcgaagg
   141721 accgtgtggt gccagttggt tggagaggat ggtccgtctg cgggatcact atggcccgtt
   141781 caaattggca tggatggaga ctttggtgcg cgtggcggac tggcgtggaa cagcgaaaaa
   141841 aggagaagca aatgcctgat cttcagcttg ccggatgtcg gccagaaccc ttggcgcatt
   141901 atctgaaggc tctaggcatt cttcgtctag taagcgaaca agctgatcct gaggttcgag
   141961 ggtggtgggt cgatgacgag tttaggctgc gcacgaaact gagccgtgag aaattggaaa
   142021 aatttttcct ggaagattac cgtccaactc cagttgtcgc accatggaac ggcggtagcg
   142081 ggttttaccc caatgatagg aaggatgcga tcgaagcaat cttctcgact cgcgcgggca
   142141 gatttctgga ttaccaggcg actgttaaac agtcaaaaga gatcatccaa ttgcttgcct
   142201 tgaaagacaa gccagatgtt gatgccaaac cgcgcctttt gcgggcttgt cgggggcgct
   142261 tttcagaaaa gtcgttggca tgggtggatg ctgcatacgt gttgactgac gaagaagcga
   142321 agtttccacc attattaggc acggggggga acgacggtcg gctggagttt actaataatt
   142381 tcatgcaacg gctgatggaa ctgtttgatc cgctgacagg acatccagag gtattgagca
   142441 aaacttggct atcttcagca ttgtggagcg aacccgtaac tggtcttttg accgataggc
   142501 ccatcggtca atttttccct ggagcggctg gcggagccaa tgcgaccgtt ggattttctt
   142561 ctcgctcact aatcaatcca tgggatttcg tgctcatgat tgagggggct gtattatttg
   142621 gatcggccgc aacaagacga ttgcagggta gcggaggtag tgaagtcggt tatccgtttg
   142681 taacgcgcgc cgcaggagtg ggctacccaa gtgccagtgg ctcagatgaa actccgcggg
   142741 gtgaaatgtg gatgccgatc tggactgcac ctgtacgcat atcggaagtt gctgccctat
   142801 tttcagaagg gaaagcgcaa gttggtcggc gtactgcgaa aactggagtc gatttttccc
   142861 aagcgattgc agcgctgggg accgaccgtg gcgtgcaatc tttttgccga tatgggttcc
   142921 aggtgaggaa tgggttggct tattttgcga cgccgcttag tcgatggcgt gtcgtttctc
   142981 gtcctgaggc gaaccttcta ttcgaatttg accactggct tgttcgattt cgacgggagg
   143041 ctactggaaa tacctctcct gccagaatgg ggcaggtact tcgggctatc gaacgggcca
   143101 taatggagta ttgccaaaca ggccatccgt caagaatggc gaacctcttg atcgccctcg
   143161 gtgcggctga agaggccctt gctcgatctc ctcagtttcg aggaaagcat tttctcaagc
   143221 ctctcccact cctctcgccc aagtggctag acgcggcgaa cgacagtagt gtggaattta
   143281 ggttggctgc atccctggcc tctgtgggcc tgcgcgagaa tatggagcct gtccgagttg
   143341 aagcagcgtg ggctgggtgg ctggataccg atacccctcc acgtattgtc tggggccatg
   143401 gatcactgac ggacaacctg attgctgtgc tgtttcggcg ctgtatggat gcccagcggg
   143461 aacagcgaaa ggggttaccc ttagcaggga gatatacgac atccctcaac gacattcacg
   143521 agtttattgc cggcaatgtg gatgaacggc gactagaggg gcttctgcgg ggactcacgt
   143581 tgatcaattg gcgtctggtt cgagagtcaa cacaggcaac ggacgaacac gaaagcctgt
   143641 tgcccggact ctatgcactg ctcaagttga cccatctgcc caatcctttc agaggtaact
   143701 cgatcccgta tatgccggca atccttgttc gtgctgctgc tggagacgca tcacaggcct
   143761 cacggcaggc tattcgacgt ctgcgcgggt gcggattcat cccagcggtg gaggccatct
   143821 ccgaaccagc taatgtcacc cgtcgcattg ccggcgctgt tctatttcct atctcaaaac
   143881 cgcaggaggc ctcgctggct gaagtaattc tcaggacaca gtaactagga cgtgacgtag
   143941 cactgtgatt tctgtttaac gtgaaaggag accgtgcatg gcttcagaag tgaatttcag
   144001 cgaactgcag aatgctcccc ggttattgat tgaagctgac ctacagccca ttcaaggcac
   144061 gcgctttcaa ccgacaggat ttccagattt gggggcggca acgtaccaat tgcacgatgg
   144121 tacagagatg ctgttggtgg aatcgccaca gagcatggcg aatcggctcg aaacggtctg
   144181 ctgggatgaa agtgccggtg atcttgttaa gcccctggct ggccttccct atattgcggt
   144241 gaaggaggat gaaaaagtta tcacgaattc tttgctggaa tcgcatcgga ttaactcgcc
   144301 gtatattctt gaggggaggg acaagtcttt ttttgagaca ctaaaaaaag agttaggggc
   144361 aatggagaaa agacctgttg accttcagat gctggcctca gtgcttttga agtatgacgc
   144421 caatgcgctg ctccacggag tttttctcgc taagagtgac ttggctggcg gcagactacg
   144481 cctccccagg agcttatccg cgtttattga ggccaaaggt agcatagtag ctgccagcgg
   144541 gggggtgaaa atggatcgtg ttgatccgca aggcgatacg tcgaagggct ttggtcatgt
   144601 tccttttcat cggaatgaat ataccgccga gaagatcagc gcatatttca atcttgacct
   144661 gatccaagtt cgaggatttc ggctgggaga caaggtggaa agtctgctca ttgccctagc
   144721 attgttcaaa gttcagaagt tcttggccga aggtcttcgg ctgagaacgg cgtgcgacct
   144781 cgaagcggtt tcaatctcgg caaagaaacc ggctggtttc accatgccct cgctgaaaga
   144841 catcgcagcc aatctccctg ctctcattga ggccgtcgcc tccgagaata agtttgcaaa
   144901 acccgccatc acctccgttt cgtataaggg agataagaaa tgattgccct gaggttccgc
   144961 ttcacagctg gtcgctatca tgcgactccc tgggggcggc atgtaaacga gggcgctgtc
   145021 gaatggccgc cgtcgccctg gcggattttg cgggcgctga ttgccacctg gcatcgtaag
   145081 ttctcccgag agattcctga acactcattc agaccgttga tagaacggct gacggatctc
   145141 cctgtctttt atttaccgaa ggccgcgttg ggacatacgc gacacttcat gccgcaaaaa
   145201 gatcccttgg gcgcagacaa gaccaaaatc tttgacgcct ttgtatccgt ttatcctgat
   145261 gatggcttca ttgtggcgtg gcctcatata accctatctt cagaagagcg agaggtgcta
   145321 acgagcctgg cttctggcct gagttaccta ggccgtgcag aaagttgggc aacggccgag
   145381 atgttggatc aatgggatgg tgacatcaat tgtcgtccga ttgagggcgg agtgtcagtt
   145441 gaaggagagc gagtgcgtat gcttgcctcg atgacaccag acagctttgt cacatggaag
   145501 atgggttatg agactaaagt tgttggggaa accaccatag tgaccaaagc ggggagaaag
   145561 aagaaggttt ctcagagtcc ggttccccct aatctttgga gtgcgttgca tgtggagact
   145621 agcgatcttc agaagcaggg ctggtccgct gcacctggaa gtcggtgggt agattatgaa
   145681 agggcggacg atgcttttcg tgtgacgtat caacgaaaac caaagatcga gcaaattcgg
   145741 ccaacggttg cccgctttgc tctgtcgagt gccgtgttgc ctcgcttgac tgatgtcgta
   145801 tttcttgggg agcggatgcg tcaagctttg atggccaagt ctcgtggact caacggaagc
   145861 gagcatgctc ttcccgtgtt ctcaggtaag ggcgccgatg gccttcgatt agatgacgat
   145921 cattcacatg cattctattt gctcagcgat gaggatggcg acggacggat tgaccatatg
   145981 accgtctatg ctactcaggg cttcgacaag catgcacaac aagctattgg catgctcagg
   146041 aagctttggg gggcaggcgg acatgacatt cacacggtac tcattgggat cggtcaacca
   146101 caggattacg gcggattttt gatgcgtgag ggagaggctc ctgcactggc tcaatctcga
   146161 atctggcgat cagtgactcc ctatatccta acccgccatc ccaagaagaa cggcaaggat
   146221 tctcccgcag cacaggtcgc aaaagacctg ctgcgtcggg ggtttccaaa gcccgagcgc
   146281 atagaacatg tagatcacac aaagattacg ggcaagaaag tgcgctggtt ggaatttcga
   146341 cgtttccgga gtggaggcgg agggcaaatt gctgattctc gcggattcgg attcgagatt
   146401 gttttcgcgg agccggttcg aggccccatt gcccttgggt acggttgcca cttcggtctt
   146461 ggccagttcg tggcggtaca agaggcgcgt gaggcgtgat gtgttggagg cttatcgtga
   146521 gagagcgccg tcgcgccgag tgctttagag cagctctttg aaccggtcct ccgggatctc
   146581 tgcggcctcc agaatctgct tcaacgtgcc gggtttgatg ctgtgatggt gaggaatgat
   146641 cacgaggtgt ttgtcggtgg tcatcgagat gtgtttggct tcgcgtttga gggctctcac
   146701 gacacgttca tgagagacct gtggcagctt cgtcatacgc tgacttggac ggtgcgtaac
   146761 ttcttgaggc gcttcagatc ctcaatgctt tcaagatagg tcttgatggc gtctttgatg
   146821 ttctccagcg cttcttcttc ggtctctcct tgagagacac agccttttaa ggtcggacag
   146881 gagacgacat aacctccggc ttcgaggtct ggctcgatga cgatgggggg cattataaca
   146941 gaacgcttga tcgactcagg cgttgacggt tctttgacaa ctgaatattt ccagacctcc
   147001 gaccgatagg ggtgttatgg acactggatc acaggggcca tccacggggg gcgacatccc
   147061 gttgctcccc gcccggatgg tcaacgagtt tgtctactgc ccgcgcctgg cctatttcga
   147121 atgggtggac ggggagttcg ccgacaacgc ggaaaccacc gaggggcgat ttcaccaccg
   147181 gcgcgtggac caggcaccgg tccggaaatc gcgacagact gagaatcagg agtcgggaga
   147241 aaccacgatc catcagcgtt ccgtgtggtt gtcttcggag ggcctcggtg tgacggccaa
   147301 gatcgacctc gtcgaaggag agggccaggc ggtcgttccc gtggattaca agcgaggcaa
   147361 gcgcccccat acgacgcaag gcgcctggga accggaacgg gtgcagctct gcgtgcaggg
   147421 gttgttgctc cgtgagcaag gcttcacctg cgagcgtgga gtgctctact tcgtcggttc
   147481 acgcgagcga gtggaagtgc tgttcgacga ggagttggtc gctcggacgc gcgaatttct
   147541 ggacggcatg cggacgatga cgcaggcgac ggttgccccg ccgcccttgg tggacagccc
   147601 caagtgcccg cgttgttcgc tcgtaggcct ctgtcttccg gacgaagtgg gctggctacg
   147661 gcagccacat aatggcgacg tgccggcgct ccggaaactg attcctacca atgacgatgc
   147721 cttgccgctc tatatacaga cacccggcgc gaagctgggc aaagacgggg agtgcttgaa
   147781 ggtcaaagat cacgatgacg taatcggcga ggccaggctg gtcgaaacgt cgcaagtcgt
   147841 gctgtatggc gcggtgcagg tgagcacgca ggtggtgcag gaactctgta aacgcaatat
   147901 tccgctggtg tactgttcca gcggcggttg gttctacggc atgacgaccg gcttgttgca
   147961 caaacacgtg gagcttcgac ggcggcaata cgcagtcgcg gcagatcgtg aacgctgtct
   148021 gcaactcgcg cggcgattcg tgcaggcgaa gattgccaac tgtcgtacgt tgctacggcg
   148081 caatcaccgg gcggctccgg ataccgtcat tcaggatctg aaaaacgatc acgcccacgc
   148141 cggtacggtg gagtctttgg aatcgctgct gggcgtggag ggcaccgccg ccagacggta
   148201 tttctcggag tttgccggta tgttgaaaga ggcagagccg ggggcgcggt ttgatttcga
   148261 ggggcgcaat cggcgcccgc ctcgtgaccc cgtcaacgct atgttgtctt tgctgtactc
   148321 catgctcgcg cgcgaatgga ccgtcacgct gcagagtgta gggcttgatc cgtatctggg
   148381 attctatcat cagcctcgtt acgggaggcc ggccctggcc ctggacatga tggaagagtt
   148441 ccggccgctc atagccgatt ccgctgtcct gaccgctatc aacaatggcg agattcagca
   148501 gagcgattgg tttgagcgca tgggatcggt cacgctcaca ccagaaggcc gccgtcgctt
   148561 gatcgaaacc tacgagcggc gcatgggcca ggagattacc cacccggtgt tcggatatca
   148621 gatcagttac cgtcgagtgc tcgaagtgca ggcccggttg ctgggccgtt atttgctcgg
   148681 agaaattcct gaactgcccc cgtttaccac aaggtaagac atgcgtcgcc actacctggt
   148741 cacctacgat atctgcgatg ccaagcgtct gcggcgcgtg ttcaagacca tgaaaggatt
   148801 cggcgcgcat ctccaatttt ccgtgttcca atgtgatctg ccggatattg atctggtgcg
   148861 aatgaaggcc gccttgacgg aaatcatcga tcagcgcgaa gatcaggtgc tcattatcga
   148921 tctgggaccg acggagagtc acccggttaa gcgaatcgag tccatgggca tcaaggccga
   148981 atgggaagaa cgccgggctc gcgtcatgta aattctgccg gtgagcctcg cgagcgctct
   149041 ggtgatggaa aaaccttcgg cagcactcgc ggctcacaat tgatgcgaat cggcagaaac
   149101 gagtatgaat aatcacagca aggaggtgtc ccatcagcgg agaacgaact ttgtgcctga
   149161 ttaaacgagg cgctcgcaac ctgcgccaaa acaccaaggc aaacggccac ttggccggga
   149221 gctgattcca cggctcagat gccgtggccc cattgaagca tgtttcaagc gcgcaagata
   149281 ttcccccgtc aatttcagat tccacggctc agatgccgtg gccccattga agccgcctct
   149341 tcgacggccg cccgcgcctc ctccttgaga ttccacggct cagatgccgt ggccccattg
   149401 aagcgtttca gccgccagct cccgataggc ttcattgatg attccacggc tcagatgccg
   149461 tggccccatt gaagcaggct gtaaaacatc gcgtcttgtc cgacgatcag gatgattcca
   149521 cggctcagat gccgtggccc cattgaagca acaccatgga ggcgaccacg cattgccgcg
   149581 cttcgctcga ttccacggct cagatgccgt ggccccattg aagcgatacg cgtgatagca
   149641 tctgcgtggc ttcaccgaga ttccacggct cagatgccgt ggccccattg aagccgacgc
   149701 agtgccaggt gtaaacgtca cagatgcccc atcgattcca cggctcagat gccgtggccc
   149761 cattgaagcc atgtcacttc ctccagtttt tgaccccgcc tctgctcgag attccacggc
   149821 tcagatgccg tggccccatt gaagcggaac aactccttat gccgttcgca aaacattttc
   149881 aggattccac ggctcagatg ccgtggcccc attgaagcca tgtcacttcc tccagttttt
   149941 gaccccgcct ctgctcgaga ttccacggct cagatgccgt ggccccattg aagcggaaca
   150001 actccttatg ccgttcgcaa aacattttca ggattccacg gctcagatgc cgtggcccca
   150061 ttgaagccgt caacgccggg accgtgaaat gccctctccc cccaggattc cacggctcag
   150121 atgccgtggc cccattgaag cacagaactg cgcagggcgg tgagcacgtg cttgctcgat
   150181 tccacggctc agatgccgtg gccccattga agcgtggagc actacacttc gctgcaatcg
   150241 cactgattaa ggcgattcca cggctcagat gccgtggccc cattgaagca cgaaggacag
   150301 gacaaggtga gtgtcaagcg caaccgattc cacggctcag atgccgtggc cccattgaag
   150361 ctttgacagc gcgagtacgt cagaaaagca ttctccagga ttccacggct cagatgccgt
   150421 ggccccattg aagccgtgtc gatacggagc atcggcgctt gcaggtcgat cgattccacg
   150481 gctcagatgc cgtggcccca ttgaagcgcg ttgctggaca tcgatggagc gagtcgcggc
   150541 acgattccac ggctcagatg ccgtggcccc attgaagccg agcgtctgct gcgcgtacat
   150601 gatccggcag ggctccgatt ccacggctca gatgccgtgg ccccattgaa gcgccgttca
   150661 gcggcacctt ggtaaatccg ttcctggcaa aggattccac ggctcagatg ccgtggcccc
   150721 attgaagcgt actaatatac ggccatgaag tcgtcacgaa tgaccagatt ccacggctca
   150781 gatgccgtgg ccccattgaa gcttgaggtc aatggcctgg atcttctcta acttagccga
   150841 ttccacggct cagatgccgt ggccccattg aagctccaga ctgggagacc aggtcccgaa
   150901 ccggatctgc gattccacgg ctcagatgcc gtggccccat tgaagcacca tcacccgtgg
   150961 ccaagagggc accagcgcgg tcagattcca cggctcagat gccgtggccc cattgaagca
   151021 acgccgtcat cgcctcccga tggccacagt tcgaacgatt ccacggctca gatgccgtgg
   151081 ccccattgaa gcgaggaaaa tctaaggctt gccgaacatg ccgaacgcag attccacggc
   151141 tcagatgccg tggccccatt gaagccaaca cgtcatcgac aagcgtttcc ttctgcctgg
   151201 tggattccac ggctcagatg ccgtggcccc attgaagctg tcttcaggaa gtcgtcgagg
   151261 cctgaccact tcagattcca cggctcagat gccgtggccc cattgaagca cgcctcgcaa
   151321 tcttggcccc gtacctggtg agttgcggat tccacggctc agatgccgtg gccccattga
   151381 agccatctaa acatggccac caaggatcgg atctatgtca gattccacgg ctcagatgcc
   151441 gtggccccat tgaagcggtc tcttgcacct catacggtgc gagaatcgcg tccagattcc
   151501 acggctcaga tgccgtggcc ccattgaagc cccgcaccct gcgcagggcg ctccgcagcg
   151561 gatccagatt ccacggctca gatgccgtgg ccccattgaa gcgtcggcgc cgccttcggc
   151621 acgatcacgc tgggattcga ttccacggct cagatgccgt ggccccattg aagcttggtt
   151681 gtcgcggtgt gctggctgtt cattgattgc gattccacgg ctcagatgcc gtggccccat
   151741 tgaagcggag gtgttgtggt tcggagttgt gtgcgaagac gcgattccac ggctcagatg
   151801 tcgtggcccc attgaagcga catagcccac aacggcgtgg gatccgagcc agaagattcc
   151861 acggtagaaa tgccctggcc ccattgaagc catgttcccc gcgcgagtac ccgtgatccc
   151921 acgatgctac ctacgggcta ggccattccg aggcatctga aaatcttcaa tgccgattcc
   151981 agcgtaatgc tcgttcgttg ctgctcaaac gcattcacgg tcggcccgct gacgcccgcg
   152041 aggacggcca atgtcttttg cgtcaacttc tgttccttgc gccgctttac ggcttcttcg
   152101 acaaggcgtg gccaatcaag gcgaaggttc cgttccatcg tttttccatg aaggtgatga
   152161 tggcatcccg caattccggg gtgccggtgg tgctttcagc aatcgcgttt ctggccgctt
   152221 cccgccgctt ttctagatgc ccggcggcca tggcaatggc cggcttcgat aatccgaact
   152281 cttcacccaa acggatgagc gttcgcgcct tgacctcgct gatgggaatg tgggcgctgc
   152341 cgcctatcga taacgcgatg gtgttgtacc cgtataacgt cgccgatact gcgtcatagg
   152401 caggtgtgag gcgcagtccc gcctcggtgt gaaacatggc gaaatttttc aggtgcatat
   152461 cggtattccc aaggagaatg ccggcaagaa tgcgcaggta gagccggtac acctcggccg
   152521 gcaggcaacc gggcgtctgc cggatgaaat ccgccatgct tttgtggctg ccttcatatt
   152581 tggcgtggga aggattcccg agcagctgat tgaattcctc gaaatgaatg cgttgtccgc
   152641 cgttggtccg gtcgaagcgc gtgatgatca aggtcggctc ttcgaatccg gcaacgtggc
   152701 ccatgcgcag atcgacaatc tcgtcaccgg gcagaaacgc ttgcaacgcc tgcgtggtca
   152761 gatattcgtt ggccgtcaga tcgccgtgcc gcggcgaggg aaatttagcg atgtgggtgc
   152821 tgagttcatc cgttcgcgcg ggacggaatt gtccgtcccg tcggaccaga gccagtttgg
   152881 gctgaatgcc ggagagcgac gctctccccg acaggacggc gagatccatc ggatcgtccg
   152941 ggcgggcagc gtcccggtgt tccggatcgg gatcgatgac ggagacagcg cccgcgcaat
   153001 cctggccgaa ggccagcagc agttcgaagc gcgagacccg acgctttccc agcagccgcg
   153061 tctgcgcttc ctcgagccag ccctcggcca ccagattgtc aaaaaatggc ggcagccccg
   153121 actggctgac aagagtgtcc gtcctcaagg gcagcgagtg ggctatggag gggtggcgcc
   153181 cggccagata gtgctcatcg taggtaaagg acatccgttc gcccggttct tggcgcagca
   153241 cgccggcgaa gcgatcctga taatagacat tgccccagag aaggtttgcc atggtaagtc
   153301 aaagtttacc agaatctgca ctcgaatcaa tctggtaaaa catagtcaac tgcattaggc
   153361 gctgtaaatg atcgaggaaa ttatagttta ccatcgcccc cggtccagcg aggcggacgc
   153421 accatccggc tggtgttcga ggttttcccc gcgcgagtgg ggaatagcaa cggggcacag
   153481 gccggatggg ggagggatgt ataagagcgg gagaatgatc gaggtttgta tcgtcagatg
   153541 atcacgctgc ttcagctctt acggctccgc ctcttcgatg gcctcgaccg attctctgag
   153601 cgctgacacg aacgcctccg gcgcggccaa tgcctgttcg gtcagttgga actccgacac
   153661 caacacaccg tctttatcca ccacgatcaa acgataaccc tgtttcacga tgactccttt
   153721 caacgggagg cccggtctgt ttccggggac gacagaaccg aacgaaggtg agaggctatc
   153781 ttacaaggaa gccggttgtg atgcgatgcg tctggcgtcg aggaggaaat gtaggttttc
   153841 ttccggcatt gctgttagga taacttctgg ttccagcctg atcttgacgt tcaatagaaa
   153901 ggacgtatcg cttgaataca actaaattga tggctgcatg tcttgtgttg tctctcgtcg
   153961 gcgtgccgac gatttccttt tcgaaggcgc gggggggatc agggggaggg ttctccagtg
   154021 ggcgcagcag tggttcatcc ggcagcatgg gcagccgtgg ctcgcgcacc tatcaggaca
   154081 atggcgcgaa gccgatcgaa caatccacga ccgccaggca agccacgagc ccgccgcctt
   154141 ccgtcgccag cggtccggcc tcgacggcca ggccgacgcc tgctgcgcag ccctcgtgga
   154201 ttcagcgcaa tcctctgttg gcgggtatcg ccggcggtct ggccggatca tggatcggtc
   154261 atatgctgtt cggggcgacc gacagcagcg cgcgaacgaa cgaagcgggt gaacaggtcg
   154321 gggaaaccgg tcaggccggg gcttccggtc ccagcggaat gcttattttg ttgatgctca
   154381 tgggcgccgg ggccctgttt ttctatctac gatcgcgtaa gcctgcggtg gcgcctgatt
   154441 tctcaggcat cacgcggagc agtgcggtga gtggctcgct cttggacgat tcgtccgtgt
   154501 cgacactccg aaccgccaca atcgatagcg agatcacctc agccgacaag gcggcctttc
   154561 agcaatcgct gaccgatatc caaaccgcct ggggcaagca ggacctgtcc gcattgcgcc
   154621 gcctggtgac gccggagatg ctggagtatt tcagtaccgg attggccgaa ctgaccagtc
   154681 agtatcaggc gaaccgtgtg gaggatgtgg tgctgctcga ggctgaagtt cgggagtcgt
   154741 gggttgaaaa tacggtgcag tacgcgacgg tgagcctgca ttggagtgcc cgggactata
   154801 ccgtgtccct gtcgaaggca ccgggcgatg ccggttatct cattgagggg agtgcagaga
   154861 agccgatcga gacgaccgag gtctggacct ttatgcgata tcaacacggc aagtggttgt
   154921 tgtccgcgat tcagcaggtc gcgtaagacc ctgccgagcg gtcccgatgg ggacagtgtg
   154981 agctggttgg atacgggggc agtcacgcag gtggctgtcc ccgttttgtt ttcggcagta
   155041 cgcttgcttg tgcgatcgca cgacctccac gtctccggca tcgtgagatc acttcatgat
   155101 gacttcaccc cctcttcgcc gaattttcat gattgccgtt gtaccttctc tttgaaacgt
   155161 agagcggcgg ctgattcggc tgcctgcact gctccctcaa accatacatt cgtcacggcg
   155221 accgttgctg ccccgtcgac agctcgtctg cgctgctgga cgcgagcctg aagggcacgt
   155281 ctcacgctgg accagacaaa gggaaaaaga ccatgcggat tgctcacatc agcccgtttc
   155341 tggaaaaggt gtcatccgag agtcaggacc cgcagggcag gatgatcttc catttgacga
   155401 agcaattgac ccggctggga catgaagtga cggtctttgc cagtggggac agcagaacag
   155461 ccggtgagct gatccccgtc gctccgcttt ccatgcgatc ctatcctgcg ccgaaacgcc
   155521 atctcgcgga ggcattggcc atgctggcat tggagaaagc gttcgccacg ctgccgtcct
   155581 tcgacatcat ccatcttcac tcaggattgt cttgtttgcc gctgatgcgt cgtagcccga
   155641 ttccgatcgt ctcgaccgtg tacgatgctc tggacgcccc cgaagtgatc aaggtctatc
   155701 gtgaatttaa ggagttggcc ttgatcgcga catgcgcgga gcaactcaaa caggtgcctg
   155761 acttgaattg gcaagcggtc attccctgct tggacatgct gcggtggaag aacgagccgc
   155821 aggccgtgga aaagatcgcg cgcgcgcacg agacggtcta cgaatggagt acgatcggga
   155881 tgggtctccc cgaacgcgtc gcgcctcact tgcgtgttgt cacgagcgcg aaggccaaca
   155941 ccacccacga gcgctctatc gcagcttccc catactgatg acgacgagcc gatgagcggc
   156001 tgaacttcct ccggtcgtcg ggccccatct gagttcctgg tcccagcatc caggtgcgcg
   156061 tgcgtgcttg tccgagtgtc tcctctttca ctaccttctc gctgatcgaa tcgagacggg
   156121 ctgatgtatt agtatggtga tgacgatagg cccggccacg ggaacatcgc aggctattga
   156181 tttcctcacc cttcgatcat tcgatggagc atgtatcggt atgcgcattc ttctcgtaga
   156241 agatgatgct gatctggcgc aattcatccg aaagggcttg aaggaagagc gctatgcggt
   156301 ggatgtcgcg tctaatggcg agcaggggtt cgagctcggc atcgcgaatc cgtacgatct
   156361 catcatcctg gacatcatgc tgcctgtgct cgatggcctg tcactctgtc gtcgtttacg
   156421 cgataccggc gtgacggcgc ctgtcttgct gttgaccgcc cgcgatacgg tgcaggacaa
   156481 ggtctccggt ctggacatgg gcgccgatga ctatttgacg aaaccgtttg ccttcgcaga
   156541 actcctcgcg agagcgaggg ccctgttgcg acgtggcggg cctcaactgc aggcccggct
   156601 gacggccgcc gacctggagc tggatccggc gactcaccgg gtgtgcagga gcggcaatga
   156661 aatcgcgctg acgaacaagg agtacgcgct gctcgaattt ctcttgcgga acaagaatcg
   156721 tgtgctgacc aggaccgcca tcatcgagca cgtgtgggac atcagttatg accctatgac
   156781 gaatatcgtg gatgcgcata tccggtcgtt gcgggcgaag atggatcgcg acttttctcc
   156841 ggcgttgatt acgactgtgc ggggggccgg ctatatgctg gaagatgagg aagccggacg
   156901 atgaattcac tgggacgcct gatccgccgg acggcgctga tcttgatggg agggctgctc
   156961 ctcggttttt cggcgctgat ctatgtcggc ggcgacacgc tgttgagccg ctatgtcgat
   157021 gggcgtctgc ttgaacttgc ggaaacattg ggccgcatta tcgaacagcg tccggatgtg
   157081 atccgcggcg ccggtgacga actggtcgta ttgggggaga agggccgcag ccaggaagca
   157141 caacatgaac tccgggaggc cgcgcatagc gtccggatct tgtcgatcga cggacagctg
   157201 ctgtggaagg ggtccgatgt ggtgcctcgc cctcccgtgt cggcatccct cctcgagcag
   157261 gtcaagcagg gccagaccgt cttcgatatg gtgcagctgc gtgaggggtc ccctgtgcgc
   157321 cgcgtctcta tccctgtgcc caggagcggc caggttcggt acatcctgca agcggaggag
   157381 tcgctccact tttcaaagga aacgctgaag gggctcgcga tcctgttggc gctcggatcg
   157441 ggtctcgtca tcgtggtggc ctgggccggc agcgcatgga tcgcgcgaac ggtcctgacg
   157501 ccgatcggtc tcttgagccg gcgggcggaa accatgtcgg aagcagatct gggcgagcga
   157561 ttatctctcg attctccgtt tcaagagttc caccggctga cacacacctt taacgccatg
   157621 atggaccggt tccagaagag ctgcgagagc cagcgccggt ttgtcgatta cgccgcccac
   157681 gaaatgcaga cgccgctgac cgtgttgcag gggaacctgg aagtcacgct tcaaaaggcc
   157741 agatccacgg acgagtatcg cgaggcactc atcgggaatc tggagcaggt ggagaaactg
   157801 attgcgttgg cgaggtctct gctgacgctc accaaggtta ccggtgatcg gccgccgttg
   157861 caactcgctc ctctcgaact ggagcccctg cttcaggaac tgatcatcga actgaagctg
   157921 cttgccgatg accgccggat ctcactcgcc ctcgatgtgg tgcccgtgcc cacgatttcg
   157981 gcggacgcgc agtggttgaa acaggcgttg atcaatctgc tggacaatag tctgaaatat
   158041 acgccgcccg gcggctcggt cacggttcgt ctccgccgtt ccgatgaatc tcttgagatt
   158101 gcggtccggg acacaggccc cggaatcgag tcggaacatg tgccgttcct tttcgatcgg
   158161 ttttatcggg cggacaaggc cagggcacgg gagtccggag ggacaggctt agggctggcg
   158221 attgtcaaag gaattgtcga ggcgcacggg gggacggtct ccgttgagac tcagcctgga
   158281 gaaggggcgg agtttacgat ccgcttgccc cttggtcgcg ctcctgaagg ccatccagtg
   158341 acctgacgcg cctaccgttt ctgccgcctc ttcgccggtc gcgtcacagc cgtgtgctcg
   158401 gccgacatca gggtctgcgc ctgtgtgatc gcatccagca gagcgtttcg ttctttgtcc
   158461 agctcttgca cgtagggcac gccgttcacg aattcttccg ctgccgatat caaggtatgc
   158521 atcgccgcag caatctcgtc ctgtgcagca gggcgggatg gtgtggttgc acgggagagg
   158581 gcgcgccttc ttctgggccc gacatacaac tgcggggctg tcgggttatc tttttccaat
   158641 tccggtcgtc tcactcggcc acgcccgcta gtcctaaacg acaggcttgt atgagttgat
   158701 agacaaatat tcttcgtctg cctgcgtatt ccgctgaatc cggccaccga ttccgagcga
   158761 ggtcaaggcg cgaacgctca tccgtttggg tgaagagttc ggattatcg
//
LOCUS       DAORRQ010000003       145413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_3_length_145413_cov_142.023783, whole genome shotgun sequence.
ACCESSION   DAORRQ010000003 DAORRQ010000000
VERSION     DAORRQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 145413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 145413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..145413
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3_length_145413_cov_142.023783"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..2114)
                     /gene="mnmG"
                     /locus_tag="PK224_01570"
     CDS             complement(240..2114)
                     /gene="mnmG"
                     /locus_tag="PK224_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRB14458.1"
                     /translation="MSEQCDVIVVGGGHAGCEAALAAARMGVRTILLTMDPDRIAQMS
                     CNPAIGGIAKGHLVKEIDALGGEMGRNTDQAGIQFRMINTSKGPAVRALRAQCDKKVY
                     REVMQRTLRAQPALEIRGGTVDRILTRGGAVTGIVTDAGTSIHARAVVLTSGTFLKGL
                     IHIGLNHFPAGRAGEASAEHLSDCMRDFGFEVGRLKTGTPPRLDRDSIDFSVMELQPG
                     DDPPPPFSYRTLRVSLPQVPCHLTYTNSQTHDIIAKNIDRSPLFSGVIGSVGPRYCPS
                     IEDKVVRFADKDRHQIFIEPEGLDTNEFYPNGISTSLPVDVQAAILKTIPGLEQARML
                     KPGYAVEYDYFPPRQLHNSLETKLVAGLYHAGQINGTSGYEEAGAQGIMAGINTALRW
                     RGEEPLVLDRSQAYIGVLIDDLITKDAREPYRMFTSRAEYRLLLRHDNADLRLMDLGH
                     RIGLVSDEVHGKLEAKRSAIEREVQRLKETRPKITSDVKRLMEEAKIGTVAPTQSLAE
                     ILRRQEMTYEKIVAVFGGANIDDLDIAEAIQLEVKYEGYIKRQLQQIQRSEKLEHRAI
                     PDQFDYDGIAGFSGEVREKLKRVKPASIGQASRISGVTPAAISLLLVAIERHRRQPSA
                     "
     gene            complement(2128..3558)
                     /gene="mnmE"
                     /locus_tag="PK224_01575"
     CDS             complement(2128..3558)
                     /gene="mnmE"
                     /locus_tag="PK224_01575"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRB14459.1"
                     /translation="MHGALDDTICAIATPPGEGGIGVVRISGLQAVDVASQVVRLRSG
                     KSLHDLQTHVMALADVGSPGALQTVPRGTESRPHAVLDEALVVVMKGPHSYTGEDVVE
                     VQCHGGPVVLDQLCLGLISAGARLAEPGEFTKRAFLNGRLDLAQAEAVLDTIRAKTAR
                     SLAIAQSQRRGELSREVEETRSALVVALAHIEAALDFAEEDIAFVRQDELLRLLDETL
                     SKLRGLVQSGQEGRIWREGAAVAILGRPNVGKSSLMNALLRSDRAIVTPIPGTTRDML
                     EEVVSIHGIPVRLFDTAGIRATDDPVEAEGIRRSHLAWEDADLALILLDGSQPLLESD
                     RLLLRRREAAQALLVVNKCDLPSRISKEDLIRVCPVNAGVLDISAKMQIGLDGLRDAI
                     SSRLMPRGLESREGVLVTNLRHAAAFERALQGVEQARQSVEAGMAGELVAMDLRIAAD
                     ALGEITGAITTDEILERIFAEFCIGK"
     gene            complement(3580..5322)
                     /gene="yidC"
                     /locus_tag="PK224_01580"
     CDS             complement(3580..5322)
                     /gene="yidC"
                     /locus_tag="PK224_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRB14460.1"
                     /translation="MEKRVIVFLIVSLAVIIGYDYLLKGMGLLPPSEPVQVATNPSPA
                     AETGKPAGGAGDSAKAASADPVSSVNGAPASPGNATGAASEEQTVEVDTELFRAKFSN
                     RGAVLKSWELKRYSAAVEQSSTPVQLVYSGGRFKGPLSLVTGDQAVTSDLGSALYQVT
                     QDLSHLDSAHPVGHLTFRYHDTQKNVDVEKELTFHHGSYVVDIAIRTQGLTTPVDVTL
                     GTNFGIVEWGEGFIGLMGSASLVDDKVLKETPDGEAERKGDVKWSAIQDKYFLSVLMP
                     QKAAAALAKKEGDKLVSAGVRFATPESEATMAMQLYAGPKEYDTLKRLNVGLEDTIDF
                     GWFVFGSWGLVKAVAKPIFYVLRFLYEFTHNYGITIILLTMLIKLMFVPLQYKSYKSM
                     KQMQVIQPKVLALQTKFKDDRERLNKELIKLYKDHRVNPVGGCLPMVLQMPVFVALFN
                     ILYMTIDLRQAPFMLWIKDLSVQDPYYVLPIVMGATMVIQQKITPTTMDPTQAKIMLF
                     LPVFMTFLFVNFPAGLVLYWLTNNTLTITQQVVTERLFGKKWQVAAADADAVTGDEPK
                     EQKSSGKRRNSPSE"
     gene            complement(5369..5632)
                     /gene="yidD"
                     /locus_tag="PK224_01585"
     CDS             complement(5369..5632)
                     /gene="yidD"
                     /locus_tag="PK224_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRB14461.1"
                     /translation="MARHITKAAVGPFWGTLALRRLCIVLLMIYRACLSPLLGPACRF
                     HPTCSVYAQDAIERYGVLKGLLMAGRRLLKCHPFHPGGVDPVR"
     gene            complement(5575..5943)
                     /gene="rnpA"
                     /locus_tag="PK224_01590"
     CDS             complement(5575..5943)
                     /gene="rnpA"
                     /locus_tag="PK224_01590"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRB14462.1"
                     /translation="MSAGQRAVPFFLKRSRDIQHIKKNGRRVSTGLFNLQICPGLSQD
                     AMLGIVIGRRFGTAVRRNRAKRLFRELGRAVRPALVPGHAFLVFPKRECLNLPFAELQ
                     ILWRGTLQRQRLARFGEPSL"
     gene            complement(6124..6261)
                     /gene="rpmH"
                     /locus_tag="PK224_01595"
     CDS             complement(6124..6261)
                     /gene="rpmH"
                     /locus_tag="PK224_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRB14463.1"
                     /translation="MSFTFKQPSNLKRKREHGFRKRMSTKNGRKVIARRRAKGRARLT
                     V"
     gene            6759..7907
                     /locus_tag="PK224_01600"
     CDS             6759..7907
                     /locus_tag="PK224_01600"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRB14464.1"
                     /translation="MRRLTHIFRTFCSLALAGLLAMSLGGCVETVPDELVEAIEGIDR
                     DLVTLRASDISPEAYSKFSREWISLRARIQSEENIVRWPWEENELETDLESLQVEGSQ
                     LVADVNTRLQAQRTAAETKLAKVEQRLRLLNTRVGDIGSRVVLGEHPVETELLVRQAR
                     LFIEQGQFDHAIQASDRAGKILLTQTALLTNELGRYADDDLIAAWRESAQRTIEWSRT
                     HRAQAIVISKADRVLSLYKNGKKVLTYPIRLGSRGIRAKQHYGDGATPEGEYRIHRKR
                     GSGQTPYFRALILDYPNADDRRRFEEAQQSGLIPKSQHMPGLIQLHGIAQGITDQPYG
                     SILLDNPQIAVLFDQVSVGTPVTIVGALESQNSISLVLADLGDQEEET"
     gene            8083..9786
                     /locus_tag="PK224_01605"
     CDS             8083..9786
                     /locus_tag="PK224_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRB14465.1"
                     /translation="MPTRPTASLLLDNPTISKTLDDLASSHTIERIWTRDHTLWKPSP
                     TEIDNRLGWLTVLDHMQDGLAELRSFEQAAREARITDVVLLGMGGSSLGPEVLRCTFG
                     SAKGSPKLWVLDSTVPGWVRQVTKAIQPARTLFLLASKSGGTIEVMSLFAHFWELVRR
                     TKGNRGGAQFVAITDPGTGLEELARERGFWRTFTNQPDIGGRYSVLSYFGLVPAALLG
                     LDVSKLLSRALAMREACRHTTHPKDNPGAALGGMMAAMAMAGRDKVTLLTSPALNSFG
                     LWVEQLLAESTGKEGTGIVPVAGEPLASPTAYGADRLFVTLRLKGDRNAALDRSIITL
                     QRAGHPVFRLNLADRYDLGGEFFRWEFATAVAGHALGIHPFDQPNVQESKDNTGRVLK
                     EVETQGRFPSLPMLTPRQALTQLLEQATPNRYLAILAYTTPSPQLDASIRALRKAVML
                     RHHIATTAGYGPRYLHSTGQLHKGGPNSGLFLELVDSMKPDLPVPGKFYSFGTLAQAQ
                     AVGDLQSLQTHQRPAVRIALGPNPVRTIRSLIAMLTPARAGRRKPAAKKRPLPKRRTH
                     R"
     gene            9788..10549
                     /gene="pgl"
                     /locus_tag="PK224_01610"
     CDS             9788..10549
                     /gene="pgl"
                     /locus_tag="PK224_01610"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRB14466.1"
                     /translation="MSQAPAIHTFTDAQELTRAAAGLFLEVGKQAIAERNRFLVALSG
                     GSTPKALYSILANDKYAQQLDWSKVHFLFGDERSVPPTHADSNFAMANAILFSPLHIP
                     SAQIHRMRGEDPPETAAAQYETTLRHLTTAVPGQWPRLDLVLLGMGDDGHTASLFPDT
                     ASLTEQTRWVVPSTSPQGTRARVTLTLGVINHASVVLFLVAGRNKAAVVRRVLEQRPG
                     DPGPYPAALIRPETGRLLWYLDRAAASELTATTDD"
     gene            10567..11652
                     /gene="glk"
                     /locus_tag="PK224_01615"
     CDS             10567..11652
                     /gene="glk"
                     /locus_tag="PK224_01615"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRB14467.1"
                     /translation="MILAGDIGGTKTNLALYDWTTERVEPVREDSFHSADYKTLEEII
                     EEFLSAPLPKPQAEDELGDEPIEASAEGTTEAPKLPPEPIKLTTACFGVAGPVIDNRC
                     RTTNLPWFIDGATLAERFAIPQVRLLNDLEATAHGLLLLNPDEIVVLNAGAPPKKKQA
                     LALIAAGTGLGECILYWDGSRYRPMPSEGGHTDFAPNSDSEIDLLRHLRGSYLHVSYE
                     RIVSGPGLHAIYEYLRDTKKNEPTWLAEKIKAGNPAAEIAEAGLKGQAEIAKQALDLF
                     ASIYGAEAGNLALKALTLDGVYVAGGIAPKLLKKLQDGSFMRGFTNKGRYKRIMSQIP
                     VKVVMNDKTALLGAASMAAQLTSSTTS"
     gene            11664..12365
                     /gene="rpiA"
                     /locus_tag="PK224_01620"
     CDS             11664..12365
                     /gene="rpiA"
                     /locus_tag="PK224_01620"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRB14468.1"
                     /translation="MTLQFDLDSEKKQAALKATEYVQDGMVVGLGTGTTSKHLIIALG
                     ERVRSGLKIQAVPTSHDTAALARQSGIPLIESDNAWMIDVAIDGADQVDPALNLVKGG
                     GGALLKEKIVAAAAKRFIVMVDHTKLVPALGGSFPLPIEVVPFGWGSTARNIEQASGG
                     RAVLRERNGVVFQTEAGHVILDLHMPKIEDPATLEIELNQIPGIVETGLFIGRTSILI
                     VGHAQGADVTLAAES"
     gene            12362..15139
                     /locus_tag="PK224_01625"
     CDS             12362..15139
                     /locus_tag="PK224_01625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRB14469.1"
                     /translation="MTGDRTDPPVTRRHAALLATRVLTKAVVWLGAWHGATSWFFVPA
                     AWSRSNPPHNHRRPKTHTMGSSMTTHDPYRLPRHVLPSHYDLRIEPDLQAHSFSGHEV
                     VTLTVTEPTTEILLNATELEVSTATLSGEGTPPRTGTVRMDEEHQRCYITFPSVIQPG
                     AWKLSLAFRGTLNDKLRGFYRSSYKDEQGHSHSLAATQFEATDARRAFPCWDEPQFKA
                     VFAVTLAIDPALTAISNTRIVDDRQEGGKRVLRFAESMKMSTYLVAFIVGKLDATTPT
                     MARQTPVRLWSVPGKQHLTPFGQEIAVYSLNFLADYYGIPYPGDKLDLIAIPDFASGA
                     MENLGAITFRETALLLDQRTATHAEQGRIADVVAHENAHMWFGDLVTMAWWNGLWLNE
                     AFATFMEMLVVDAWKPEWERWTAFGVSRAAALSVDGLLSTRPIEFPVRAPKEAEAMFD
                     VLTYEKGASVLRMLEQHIGPTVFRDGVRHYLTTHAYGNAETTDLWVSLAHASQQNVPA
                     LMNEWIFSPGYPLLSLAIDSSSTLTLTQRRFTYAEGSTAASSGAPAQLWQVPIQLRIH
                     TARGAETRRVMLSDQENRIPLPKDWTSVLANEGGHGFYRVRYSTALLNGLQQTGLQTL
                     APVERFNLLNDTWASTIAGMVSPADYLSLTEHFRGEQDPHVWAVMLGSFSTMNHLLSE
                     EDRPLLAAFVRNRLTPTFQDLGWTPRTDERDLVKELRGDVIRALGTLGRDPSVQTQAL
                     EAYTDLQQQTRPIDPNVIPALVSILAFTGDAARYEEFLNRFHKASTPQEERRYLFSLA
                     AFRIPELLERTLAKTLTDEIRTQDAPFLVSSLLHNVYIREKAWEFVKTNWERMDRQFP
                     KSGLRRMCGGITGLSTPELEQDVRAFFTSRKIDLGGKTLEQYLEQLHIAVRFRERDRD
                     AIRAVLARVAI"
     gene            complement(15170..17842)
                     /locus_tag="PK224_01630"
     CDS             complement(15170..17842)
                     /locus_tag="PK224_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB14470.1"
                     /translation="MIPFWLMMAWRELRSAWRHFLYFLGCIALGVGAVVGVSLFSTNV
                     ERAVLKEARGLLGGDLEIRLSKPVGAAGSVVLQSLAERGILRTRVSELVAMVARIDRA
                     VGAADVTQLVELKAIESGYPLYGIVRVEPDRPLMELLRQTGHSCREACHGAVVQESLL
                     IRLGLVVGDAIKIGQASFRITGVIHTEPDRMANMFSLGPRVLISQDGLVAADLVKPGS
                     RLRERHLLKLPASMPLSPLLHELQGRLSAESARLSSYRDAQPQLKQFLDQLARYLGLV
                     GLTALFVGGIGVALSIQAFVREKLQSIAILKTLGADTKTIIYSYLGQAVGLGVLGSAV
                     GIGIGLLLQTVLPQAVSTLLATDVLQQVEFTSVLSWRALAPLSKGLGLGVLTTLLFSL
                     WPLLTIRTIKPAVIFRREVEGAIRPGVRPETSVWSRAAQVVTADPVRAVTAAGIGLGL
                     AGLSVWQAGSLMIGGLFIGGLLVAVVALTVTAKALLLGIRVLPSPRALSLRQALGNMQ
                     RPGGQTLGVMVSIGVGVMVILAIALLEQALVRQVGENRPNDSPTFFFVDIQPDQAEGF
                     SALIHRRTGDVAPDLTPLVRSRLHAINGQAVAVERESEQEDQPGQSKEEKRKNWYVNR
                     EYVLTFLEQLPKDNRIVKGAWWKPGQVFARPQVSVEEEAAKSLGIDVGTTLDLNIQGT
                     IMRAEVSSIRKVEWGNFSTNFYMIFSPGALDGAPMTYVATVRVSSQDEVALQSAVVAA
                     FPNVTAINIGEVLSSFARVLDRLSLAIRAVALFCLLAGALVMAAALAATRYRRLYEAV
                     ILKALGATRALIARSFAAEYAVLGCVAGVIGVGLASLFSWAILRYILELPWSLEPSLL
                     GIGLGCTVLLTLIVGFLSTYRLLGQPPLTVLRHE"
     gene            complement(17839..18543)
                     /locus_tag="PK224_01635"
     CDS             complement(17839..18543)
                     /locus_tag="PK224_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB14471.1"
                     /translation="MIATQHVTMQLAAGGQTVTILDDVTIDIPEKQTVAIIGPSGSGK
                     STLLGLIAGLDRPTSGTIWLNGVEITALGEQAMARLRLANVGYIFQSFHLIPTLTALE
                     NVSIPLELAGDSQAPRRAEELLHAVGLGHRMSHYPVQLSGGEQQRVAVARAFACRPPI
                     LLADEPTGNLDSSTGQQVIELIMALHRDAGTTLVLVTHDQHLAASMERVITLRDGRVA
                     SDQLASLHHRMPDLES"
     gene            18659..19405
                     /locus_tag="PK224_01640"
     CDS             18659..19405
                     /locus_tag="PK224_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB14472.1"
                     /translation="MALVLSGLSGCDQSNTSTVPSSPDSLASATPPSSEQTPPRESLP
                     ATPSPSPDDRPRIIAFGDSLTAGLGVSPEQSYPTQLQKQLDTLGYHYQVLNAGVSGDT
                     SAGGLRRVSWVLAGKPRVVILELGGNDGLRGLGLPETRSHLDAIIRQFKDAHVRVILA
                     GMKLPPNYGEEYTARFEAIYRDLAQLHGLPLIPFLLEGVGGEKALNQADGIHPTGEGY
                     RIVVENVLRSLLPVLKDASTNNSSAKKKQA"
     gene            complement(19467..19643)
                     /locus_tag="PK224_01645"
     CDS             complement(19467..19643)
                     /locus_tag="PK224_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14473.1"
                     /translation="MNKLIAGLIAGIGALQATSAFANVSEGPPDYSGITGLYYTLIAL
                     ILAFGVYDTFFKKS"
     gene            complement(19783..20595)
                     /locus_tag="PK224_01650"
     CDS             complement(19783..20595)
                     /locus_tag="PK224_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14474.1"
                     /translation="MISSVVEPNAFQIGKQYQAERRVQMTDASDTEVTSSVMGNSGLY
                     EQTIRLIDGHLEAKCSCTLSEQPICRHGVAALLEYQRWSKARVVPKPRVAAPRPEAEK
                     PAAPVVSSGDMKLSELNQFTEWMQRTVQAIRAGKPLPDQPSIGPGLVLTWTQVIQQMD
                     ERRRESEVAQVELDADIRNREAVVARLTQDLEASVNESKSLQVICRDLQREIEAQKAA
                     VSKTTDLARQVEQVEVEVKAMAALLTDKGRRLEGLSDTCRDVSSMLKSLGKA"
     gene            complement(20864..21880)
                     /locus_tag="PK224_01655"
     CDS             complement(20864..21880)
                     /locus_tag="PK224_01655"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRB14475.1"
                     /translation="MNTFTPSLFRKTRPVSGRLLALLGLVLMMFGCQAKDRSAMVTHR
                     VWDEWAVGQVVDARTGLAVPMQPWLEGLATYDVIYLGEEHHNRSHIDAALTVLRSLMS
                     QGRRPWLAMEMFGWDGQPALARYLRSEESIRSEFLEQVAWKQNWGGAFEDYEPLAQFA
                     KDQRLSLLAMNPPKSLIRQVVKQGLVQAKEQPEWRQWGMEGEVIVDAPAYRSRILSQL
                     QDCHGGGSPEDYQTMYEASMVRDEGMAKTLAAAMKQLRVEASSGQGPLLSYTGGGHVQ
                     YRLPVPDRVARRVPEGLKQVTVYMATFERERAAELHQAMQEGIADYVWLTPQGAQGPP
                     RRCR"
     gene            22020..23348
                     /locus_tag="PK224_01660"
     CDS             22020..23348
                     /locus_tag="PK224_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14476.1"
                     /translation="MHVDSDNLAAFRKQITSARSLDAQAWEASRHLVNAAHWPMTLQA
                     LVNAIETSTVPDTIKRSLVEALPPEHESTNRIASTESLKHLTGLPPSKALRALCIFFG
                     VRSKPSSKWPLPAVTADTIDVLIRRHHNPFDLLTQENPASVLDLGAGDLSFAEELATL
                     YEPQLAAQSRPLIFHCLDRLDPGSQLGGPLHAHPLRLNRLRSRPGLQFRFYGDQDMFA
                     LAPLEQDHRLAERYLIVTCWAPATPTFAYEPTRLSPACLAEELRRTKGESRQVRHGKE
                     SALEVQHAGRSLLFPPWKFDIRGPLALLELMATRGALCVLGAVDSQVFWEILSQLIED
                     PRVRPRDLILSAQNVPEVFGDTYHKLSALPIGGSCLLSDLTPLRRTFPSVLHRPADRT
                     AAYRFRQVTIQRGALFEGRPASSTARRFQGMAEETPPWFLTLVPEPVQTA"
     gene            23507..24448
                     /locus_tag="PK224_01665"
     CDS             23507..24448
                     /locus_tag="PK224_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRB14477.1"
                     /translation="MNSSQSIKSLQDNIAQVIKGKPQVIEMAVVCLLARGHLLIEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGISMFNRQKQAFEFMPGPLFANIV
                     LADEINRTTPKTQSSLLEAMSESQISVDNQTYPLQQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPTPDEEKKVLDRPQLLHPADEIQPVLSAQHIVDLQAQAEKVRMED
                     SLMDYLLAIVLATRHNELLSLGVSTRGALALCRAAKALALVRDRAYCLPEDIKELAPT
                     VLSHRVMLSRSQGMRTNTVEHTEQVIQDILETVPVPV"
     gene            24526..25668
                     /locus_tag="PK224_01670"
     CDS             24526..25668
                     /locus_tag="PK224_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRB14478.1"
                     /translation="MATNKLQALLRRLFRDRTIRVTTEGIRFLLLTLAVGIAAVNTGN
                     NLFYLLLAMMLSLVVLSGLLSEQCVRRLDIHRHLPDYLFVNQPTTATLRVANHKSRIP
                     SVSLRLLDVVAGKDLDRGIHITHLAPAASTLCSYPLLVRQRGPYRLDGIRIVTPFPFG
                     LFYKKSFYPLEATLIVCPEIIPLPPLHLQELNTLGQDHARARRGPGNSLYNLREFRPG
                     DDSRAIHWMTTARTSKLMLKETEAEERRSITLAISTVAPDEAENSFERALSIGASLID
                     HFLKDGYQVRLLLGDQQDILACGTDQALHLFHALALCERGPMATGAAIRHSMARALAE
                     LNEGPTILLSPWTDPARNEQFPSVDYIVSPQSHRDLFDDTGSSLSA"
     gene            25640..27844
                     /locus_tag="PK224_01675"
     CDS             25640..27844
                     /locus_tag="PK224_01675"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRB14479.1"
                     /translation="MTLDRAFRLSSILLAATGFASLTLAIALPFWLLALAGAAFAVAL
                     LRVNHAPGFSELFPHLRLSALTWNIFLLLAFAGFWIDLLLVSQELLPAGIHFLVLLLV
                     NKLSNLDQRRDFLHLYAISLITLLASAALTTQIWYAPFFFVYLVAGVWTLLLYHLLQE
                     HEESTGHHLLGSQAAPLDSTRPHITARFFWTTNAMAAGAFGLTLFFFFSIPRVGVGLL
                     QHSHGESLRTTGFSEQVDLGVMGPVKQDPSIVMRVELPDSPGTPAARGPLYLRGVAYN
                     RYDGKSWSNNLLHRRMLTELPQGTFTLRTPGAKPPPQARALRQDILLEPLDTAVLFGA
                     PLAFAVKGDFHSVQSDLMGSLHLAFPSHSRIQYTVYSVPTSLNPAERAAAAVLYPEFI
                     LQQYLQIPPVSPQILDLAQHVTQSATSVSQAVTLIHTHLLANYRYNLDVPSLQSAHPL
                     EDFLLTRKTGYCEHYATAMVVLLRATGIPARLVTGFLATEWNEFGGYYTVRQRDAHAW
                     VEVYFPRSGWITMDPTPPAPENIGQTWWQSAGRVMDSVRLQWDRLFVHFSANDQLTVV
                     QGIREGGEAVRTKLSESLNSLSAQSSAMFRRFMSSFTSTGIPETVLLIILAIGATYAG
                     RRLLRSSRNTTAPPDVLSAHQRTVVTLYTAMLHCLAQRGIVKSASATPMEVLRHVRDE
                     WAEAWPSADALTRLYTRVRFGHAPLTPEDHTAAENLLRRLHTLERATTRSQQ"
     gene            complement(29120..29725)
                     /locus_tag="PK224_01680"
     CDS             complement(29120..29725)
                     /locus_tag="PK224_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRB14480.1"
                     /translation="MEDHLPVDHPKVIGAYLWQQIPQPTYPTKAQQRGWQGVVLICVV
                     FLKDGTITEAKVHQSSGIPLLDNNALDAIQSMKRLNLDLDLHRPEYSAVIPFRYHLQP
                     MTEHQRKIAANVWACLTAQAAKPSQLIGLASAQSGTVIMRVTLRTNGEVEEIKLDESS
                     GSDVLDQTATKLVMDCSPYPLNSTDAHKPTVFVVPFSYNIR"
     gene            30169..30417
                     /locus_tag="PK224_01685"
     CDS             30169..30417
                     /locus_tag="PK224_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14481.1"
                     /translation="MKGEDTKTETLQFKVTEQERRLIERCAQEEGTTVSKYVRGAVLM
                     SMVMDGKAEAIKIVAREVGEKAFAVVRQKLTRPAQEGR"
     gene            30563..31567
                     /locus_tag="PK224_01690"
     CDS             30563..31567
                     /locus_tag="PK224_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB14482.1"
                     /translation="MDSGFTLTIDWLAFTVLASNPEETMRVLGGDWSKAKGGFRGYPL
                     SWMRSDGLRGVGKLGTNAPRRPNEIHVDLSGGLASALTLDQIRTLLQWVHKQQGHVTR
                     IDCALDDRAGTVPVRTIREAVAAGQCVTRAAQVRHIVSNLTHGTGATTGETMYFGSPQ
                     SQTLLRIYDKRLELQSKGQENWPEYGTRWELELKKDRAEQCARALATLDETDWKELVI
                     GLLRSYVDFRQTSKDAEDEERYRAPVLEWYALLTEGFQKGRLAQEQQVQTLQNVKRWV
                     SDTLTPMLAVICATPGGEEWLLEEIVRGISRWKDRHRNLLKQPARFHRSAGGHAGSPC
                     "
     gene            31610..31921
                     /locus_tag="PK224_01695"
     CDS             31610..31921
                     /locus_tag="PK224_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB14483.1"
                     /translation="MLNVKELSAWLNIKPSTLYLWASENKIPCRRIHGLVRFEPEAIQ
                     AWLKGFETNSVKPFSLPTRHYTRDLDQLIEASKREVYTSRHGETIQSSLIQKEDADGA
                     V"
     gene            31908..32981
                     /locus_tag="PK224_01700"
     CDS             31908..32981
                     /locus_tag="PK224_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB14484.1"
                     /translation="MGLFRRNKVWWMTFIHQGQQVRRSTGTTDKRLAEAILGKVRVKI
                     VEGRFFDTLEAKDHTFNDMMDRYLKERSITKAPASRRRDEQCLTHLRSAFGSLNLAEV
                     TPKLLAAYKAKRRETAKPATVNKELGLVRHSFNVAIREWEWCRDNPMQRVCMEKVRNE
                     RDRWLSSDEEGRLLAHSAPWLQDIIVFALNTGMRRGEILNLQWQAIDLNRQVLVVMRS
                     KNGEKRTVPLNGRLTDLLKRKASKRAAGSFVFASSAETLFDARNLTRSFRLAATKAAL
                     SDFRFHDLRHTFATRLAQAGVDLYKVQRLLGHKTAAMTQRYAHHSPESLREGVLILER
                     VHPQISQNYHNEGGAVGECCASA"
     gene            complement(32988..33062)
                     /locus_tag="PK224_01705"
     tRNA            complement(32988..33062)
                     /locus_tag="PK224_01705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33028..33030),aa:Gly,seq:gcc)
     gene            33346..33675
                     /locus_tag="PK224_01710"
     CDS             33346..33675
                     /locus_tag="PK224_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14485.1"
                     /translation="MTSRPQPSAPFAAQAIPFDEYLASGKIPEGLLTSEYIGQQFVER
                     LVHYVLSVPAGSYTMAQLSRLLEELDPRAQVFFFKRLKENSPDSLKDFAPLYYGFMNE
                     FHSLLFT"
     gene            33760..34128
                     /locus_tag="PK224_01715"
     CDS             33760..34128
                     /locus_tag="PK224_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14486.1"
                     /translation="MNPTLQRAVTSFYNLVYEAQTFATTMSRIDTAEQEHYAGRIEGL
                     NWVLDRCQELEDMDANITPTTLQRVLTEVKSDLDHELSVQRREKGRRADGREEALTFV
                     ADYLSSLITATEIESAKTSV"
     gene            34186..34425
                     /locus_tag="PK224_01720"
     CDS             34186..34425
                     /locus_tag="PK224_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14487.1"
                     /translation="MAREWIIFAVCLGLGGHIALAVVLHAPDLWPWSKAWLYGLLSGV
                     TLYVVVQVIRSLWWIFRGRNAEEPETPEDQRIGHP"
     gene            complement(34936..35232)
                     /locus_tag="PK224_01725"
     CDS             complement(34936..35232)
                     /locus_tag="PK224_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRB14488.1"
                     /translation="MRDIVSDAAGKKVNLDKDLLAILCCPETKQAVALADEQLIQKVN
                     GAIERGALKNKAHKPVTEKLDGGLIRSDNKILYPVREDIPVMLIDEGIPLDQVS"
     gene            35376..36002
                     /locus_tag="PK224_01730"
     CDS             35376..36002
                     /locus_tag="PK224_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRB14489.1"
                     /translation="MTASHRLGRIFLTGLLVLLPAWTTFLILAALFETLDSFLLNLIG
                     RQIQPYAPGLGLLLLIGMVLTVGAIATQVIGQRVVRWTEAVLDRIPLIRSIYMTLKGM
                     TDLLNYRARFGQSTVVAFPFPRDGLWALGFVMGSPPAALQIAPMVELVMVFVPTAIHP
                     FTGYLALIPKTQLHPLNLLPEEALKLEFSAGLYRPLPGWLTSPARNPS"
     gene            35999..36517
                     /locus_tag="PK224_01735"
     CDS             35999..36517
                     /locus_tag="PK224_01735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB14490.1"
                     /translation="MSLADSLTVRPATLEDLDALTTFSAAMALETEQRTLDHARLRLG
                     TRAVLEQPERGRFYVADLRPEPPADRVTVGQLLLTYEWSDWRNAQFWWIQSVYVHPDW
                     RRKGIYRSMHGAIINLARSQPGVCGVRLYVEGDNHIAKCVYERVGLSPSSYQVYESDF
                     VLPTPHSARTKA"
     gene            36553..36933
                     /locus_tag="PK224_01740"
     CDS             36553..36933
                     /locus_tag="PK224_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14491.1"
                     /translation="MRRVSVLLVLMLLTLNACAFSRGTLGDEIKTETVNSIKKGVTTR
                     SEVLALLGAPDRLLQLNGRDLFQYYRYDAKVGSLLLIVLNFSRVSVKSDDLFVVINRE
                     GTVEEVIASKRTEGLEFRFWPFGD"
     gene            36938..37360
                     /locus_tag="PK224_01745"
     CDS             36938..37360
                     /locus_tag="PK224_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14492.1"
                     /translation="MTGWNRSVTLLFSAVIALALSGCNVARLTINTPLTPESVAFIAE
                     GRTTLADVVDTLGAPDSITDTETGTIVTYRFLNAKYSRVNFGWLLKPWSPVDPDLVFS
                     RSGLGTDAFELLCDDKWIVTHHAFVRHRPAPPFTPYPF"
     gene            37428..38285
                     /locus_tag="PK224_01750"
     CDS             37428..38285
                     /locus_tag="PK224_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRB14493.1"
                     /translation="MGAGSSDESSSQQAPGTSSSYIPADHDIDFLQRDELRPLRLGLE
                     LLKPELIQTEQGIRSTIVVFGSARLLEPSRARQALEQASAALQACPDDRTCQQRVAVA
                     ERRLALSRYYDVAREFGRLVSSTCQIDGQCDYVIVTGGGPGIMEAANRGAADAGAKSM
                     GLNITLPHEQRPNPYITPDLCFQFRYFALRKMHFLLRARALVVFPGGFGTLDELFETL
                     TLLQTGKTRNITIILMGQAFWERLINWPLLIEEGLVGPEDLALFHFAETAQQAWDLIS
                     RSHGGPSAR"
     gene            38282..39679
                     /locus_tag="PK224_01755"
     CDS             38282..39679
                     /locus_tag="PK224_01755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB14494.1"
                     /translation="MKLSFHGAARSVTGSRHLLEMPGNRILLDCGLFQGQRQEADRQN
                     RFLGFDPRSIQAVLLSHAHIDHSGALPVLGKHQFRGNVHMTRATVDLAAVMLEDSARL
                     QENDCAYLNKKENRRGKRCLQPFYESRDARAIIRRFVGARYGDTIKVTPRVTASFHDV
                     GHILGSAAIRLKYSARGNSTTVLFSGDLGRSQMPILRDPAPPPSCDVLIIESTYGDRL
                     HEEAGEALTQKAQQLVAHAKAHKSKIIVPAFALGRTQDLVMRIKRLVAEGRIDPLPIY
                     IDSPLASKVTDVFRKHPECYDEETFRTFTSEGDPFAARYIRYVSSPEESKRLNEMKGP
                     CVIIASSGMCEGGRVVHHLKHGIQDEANIIAIVGFQAEHTLGRRLVEGWDVVPIFGIP
                     TPRRAQVVVFNGLSAHADRNDLLAYVRAISPAPQQVFVVHGEEKQALSLGAAIQTEHP
                     GMAVVVPAKDSIYEI"
     gene            39724..40989
                     /locus_tag="PK224_01760"
     CDS             39724..40989
                     /locus_tag="PK224_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRB14495.1"
                     /translation="MNPSRTILNAVRACAVILLTACVSPAWCLDGSPDSVDQVRIRAR
                     ALGLHPGQFQPGPLNAITDVPGVKVGQVTLMQGDGPLQPGQGPVRTGVTVIVPRDDVW
                     HKKVPAGAFVLNGTGEMTGLSWVAESGFLEYPIALTNTLNVPRVANGVISWMLKQYPG
                     IGITDDTLTPVVAECDDGRLNDIQGRHVSETDVMRALDEASSGPVAEGSVGAGTGMIS
                     YGFKGGIGTASRRLPEANGGFTIGVLVNANHGRRPELTMGGVPVGQRYGAALTQSSLS
                     PEQNSSSLHASREGTSGNAEGSIIVVIATDAPLDGRQLTRLGKRAALGLARTGSTARH
                     GSGDFMLAFSTGNVIPHYPSDPTFSLTHLADTHLNPVMTATVEATEEAILNALTGATT
                     VTGRDGFRAEAISIPRLRELLSAGATPGP"
     gene            41085..41312
                     /locus_tag="PK224_01765"
     CDS             41085..41312
                     /locus_tag="PK224_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14496.1"
                     /translation="MADYPIGGGLTLLTALEKNGKFAEFLQTRMTQALETQDPTELHY
                     LLAQLDDYHSYMWRYYKKLASDRPERMNPGV"
     gene            41361..42587
                     /locus_tag="PK224_01770"
     CDS             41361..42587
                     /locus_tag="PK224_01770"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRB14497.1"
                     /translation="MILTPPSTLRFASALTRQADAQAAADELIRAIRVQLGSSRIDVA
                     FLFISAQHTDQADTLSHALRTALGPDTLVGCTGEGVIATGREVETGPAATLWAAHLPG
                     VIAHPLRLSFSSVHDQFSLRDWPELDYGGESAPVMLLFADPFSTPLQDVLGVIEERYP
                     HARALGGLAGGGQDLAENRLFLDDEVYSDGLVGVALSGNISVRTVISQGCRPIGDRFI
                     VTKAEHNVIQELGGIPALHCLQTVFGQLSMDERAQAQRALHIGIAMDEQRAQFTRGDF
                     LIRNLLGADQQTGAIVVGDVVQEGQTVQFQVRDAQSADEDLHALLAASRLEESQRPLG
                     ALLFSCCGRGKGLFGVPNHDASVLGEQLGAIPLAGFFAQGELGPVGGRNFLHGYTASI
                     AIFSEPDRSDTQGSNH"
     gene            42719..43090
                     /locus_tag="PK224_01775"
     CDS             42719..43090
                     /locus_tag="PK224_01775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRB14498.1"
                     /translation="MAQSEQTASTPEVTTVSGLTYTDVVVGTGREAAAGNLVTVHYTG
                     WLTNGKKFDSSVDRSEPFSFPLGAGRVIKGWDEGVAGMKVGGKRKLTIPSQLGYGARG
                     AGGVIPPNATLVFDVELLEVR"
     gene            43146..44255
                     /gene="gcvT"
                     /locus_tag="PK224_01780"
     CDS             43146..44255
                     /gene="gcvT"
                     /locus_tag="PK224_01780"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRB14499.1"
                     /translation="MKQTPLIDAHRKAQGKLVDFAGWEMPIQYSGVVDEYQTVRRHAG
                     LFDVSHMGRISVSGPGSLAFLQRVTTNDVSKLSVRQSHYSMICAPNGGIKDDIFVYHV
                     KPYEFLVCVNASNREKIVAWLHEKVAQAQGCKVQDQSASLAQIAIQGPASRDILATVG
                     LADLSTLKIRHCLDATLCGHATLVTRTGYTGELGYELYLPAAGAPNVWDRLLEAGRPL
                     GIKPAGLGARDLLRLEMAYLLYGNDMNEETTPIEAGAEWVVKFDKGDFIGRTELLAQQ
                     SQGAARRLVAFELVEKAVPRHGFKILSAQVPHAEIGEVTSGNLSPLLQKGIGMGYVTP
                     AAAQPGSSILIDIRGKTCPAVVVKPPFYKKPAGTP"
     gene            44259..44777
                     /locus_tag="PK224_01785"
     CDS             44259..44777
                     /locus_tag="PK224_01785"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRB14500.1"
                     /translation="MVKPIYKGTVVTLNVDTVRLPNGHTIDLEVIRHPGASAVVPLKE
                     DGTVVLIRQFRHAANGFIYEIPAGKLHPKEDPLDCAARELEEEIGYKAGRFELLSSIF
                     TAPGFADEVIHIYLATGLTIGTQNLDQDEVLEVVEMPLREAIAKIEDGTIRDGKTIVG
                     LQAVYIRQEQYR"
     gene            complement(44854..45078)
                     /locus_tag="PK224_01790"
     CDS             complement(44854..45078)
                     /locus_tag="PK224_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14501.1"
                     /translation="MKKMMLTVMAAALLVAFSAPSFAGDDKKKEEKKGGHFSQTLFGE
                     EKKKEEKKGGHLSQTVFGEEKKKDEKKGGH"
     gene            45318..45863
                     /locus_tag="PK224_01795"
     CDS             45318..45863
                     /locus_tag="PK224_01795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HRB14502.1"
                     /translation="MPEQPPLPDHLRPDLSIVFVGINPGLRSAALGHHYAGPSNRFWK
                     LIYDANLVPEPLSYRDDGRLPEWGLGLTNLVSRSTASMESLTPQDYTAGRLVLMRKLL
                     RYRPRVVALLGMTLYPILFPLEPKQTGRKPGLQPATLYDSNIVLLPNPSGRNASYPYH
                     SMLEAFRTLTPWAKSGLKSPP"
     gene            45963..46604
                     /locus_tag="PK224_01800"
     CDS             45963..46604
                     /locus_tag="PK224_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14503.1"
                     /translation="MQCFPITMSLAIALLFVAEPASAGASVQQLTAGALHECALGRQA
                     QTRAERVQHFAQGQAFGEQAVEKDDLSPDAHFSLFCNLGEQLRIDGESLTSLFGFRRM
                     MKELNRTLELAPDHLDALSAKGTVLVRIPAFMGGDKEKGETLLRQVIAREPAAVNARL
                     SLAKSYCANGRHEEAVAIATKALDLAQSLQRADFIPEARQVLSQLRAQAAKGN"
     gene            complement(46647..47576)
                     /gene="rbsK"
                     /locus_tag="PK224_01805"
     CDS             complement(46647..47576)
                     /gene="rbsK"
                     /locus_tag="PK224_01805"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRB14504.1"
                     /translation="MILVVGSSNIDLVASVDRLPSRGETVLGYRFAQSFGGKGANQAV
                     AAARAGAEVAFLSKLGADANGRWIEQHLAAQGLSRPILLRDAEFPTGVAMILVDLSGE
                     NQIAVVPGSNGRLTPADLRQHRELIAEARVLLLQMEIPRETVFEALRLGRECGLTTIL
                     NPAPAAPLPSDLLRLVDILTPNESEAQVLTGSTDPAEAAQILTDRGVGTVVVTCGANG
                     AFLTVGKKDVTHIPGFLVETIDSTGAGDAFNGALACAVAEGVPIKSAIVRANAAGALA
                     TTGRGAQESMPTKDDIEQLCRSGIRQLEPPPRS"
     gene            complement(47573..48559)
                     /locus_tag="PK224_01810"
     CDS             complement(47573..48559)
                     /locus_tag="PK224_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRB14505.1"
                     /translation="MQRPSPTKVILDTDPGVDDALALLLAFASPELQVVGVTTVCGNV
                     PVGQATKNLFRLLNVVQPPPGLLVGQGAARPLEEDLVTAVQVHGSDGLGELDSVLAEE
                     GTPRYPQVRLPSVLSTAQDVWNECVRRHPDEVTLITLGPLTNVAVALKVNPLTVQKFR
                     SVIVMGGAIGVPGNVAPAAEFNMYVDPHAAHRVFQASLPLTLVPLDVTTRVGVTRESL
                     MTWAAESRAPLARMTTDMTRKAFEFAEKVEGHGLFYFHDPLAVLAAVDSSLLKVEPLH
                     VSVEMAGRVSRGITIADRRPRTPEEKARPNMRVAVDVDVDRTLSFLRNRLCP"
     gene            complement(48592..49290)
                     /locus_tag="PK224_01815"
     CDS             complement(48592..49290)
                     /locus_tag="PK224_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14506.1"
                     /translation="MATATSFAPVLRWIPLPNRSVGGASSTDTATYRLPQVHAVLSIA
                     AGCLLLPLFFSPPAWADSESGAAAEMQQSSPMLDVDPGVTYRAGARLRIPGTDWSFLV
                     PTGWQSTRPEDSEMPFLMAEEGKGLGMMFPLTDVTRETVRDHLSQPLSLLHGLSFIPA
                     GSEVETELSIARSYQGEDLAGRALAVLGPGNTSVIYFLMGPPDEASGYQSVLEGLGQA
                     TRFAAPVSGRDVGL"
     gene            complement(49238..52123)
                     /gene="gcvP"
                     /locus_tag="PK224_01820"
     CDS             complement(49238..52123)
                     /gene="gcvP"
                     /locus_tag="PK224_01820"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRB14507.1"
                     /translation="MPQPNFLEPNDTFVPRHIGPTDSDIQEMLATLSLPSLEALVEVT
                     VPSDIRLQTSLTVPSPRGEQQVLAELRGLAGQNQVWRSLIGMGYYDCITPLVIQRNIL
                     EDPGWYTQYTPYQAEIAQGRLEALVNFQTMVADLTGLPLANASLLDEATAAAEAMTMC
                     AAMSRAAGHERKKFFVSENCHPQTIAVVQTRAEPLGIILQIGAIQSLDLSRDEFFGLL
                     LQYPSTDGYVGDYSEFITRAHASGAYVVVATDLLALTLLRSPGEFGADVAVGSSQRFG
                     VPLGFGGPHAAFLATKEEFRRQMPGRIIGVSKDVTGRVAYRLSLQTREQHIRREKATS
                     NICTAQVLLAVMAGMYAVYHGPAGLRRIAERIHGLTMVLAEGLRRHGCAVGLEPVFDT
                     LRVPLSPAQSDTILNRARQQKINLRQYDDQSLGLSLDEWSTVEEVQQVLALFVGHEIP
                     AEEFQAILASVDVRYPAPLARTSPYLTHPVFHRYHAEHELLRYIHRLQSRDLSLVHSM
                     IPLGSCTMKLNATAEMLPVTWPEFGRLHPFAPSEQAQGYQTLFQQLEAWLAELTGFAA
                     ISLQPNAGSQGEYAGLMVIRAHHRHRGETQRDVCLIPVSAHGTNPASASMCGMTVVPV
                     ACDERGNVDLNDLEAKATQHRNRLAALMITYPSTHGVFEEGIRRMCQIVHTHGGQVYM
                     DGANMNAQVGLCRPADLGADVCHLNLHKTFCIPHGGGGPGMGPIGVARHLVPFLPGHP
                     VTKLGGPESIGPIAAAPYGSPSILTISWVYIALMGREGLTKATQVAILNANYMAKRLE
                     KYYPVLYTGTRGFVAHEFILDLRPLKESSGVEAMDVAKRLMDYGFHAPTVSFPVAGTL
                     MIEPTESEVKAELDRLCEALIAIRGEIQSIAEGRQPRAGNVLKNAPHTALSVTAAEWT
                     KPYSREQAAFPAPWVRDNKFWPSVGRIDEAYGDRHLFCTCPPMDPAS"
     gene            52328..53983
                     /locus_tag="PK224_01825"
     CDS             52328..53983
                     /locus_tag="PK224_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRB14508.1"
                     /translation="MITHDILIVGAGLAGMRAAIAAPPDLNVALLSKVHPVRSHSVAA
                     QGGINAAIGEQDSWEAHAYDTAKGGLYLGDQDAIEAMCREAPQDILELERMGVIFSRT
                     PEGRIAQRPFGGAGFPRTCYAADRTGHALLHAMYEQLMKRRCRVYEEWYVTALLVEQG
                     RCCGVVAWDLVHGGLQVLRAKAVILATGGSGRVFSTSTNAVINTGDGMALAYRAGLPL
                     EDMEFVQFHPTTLKDTGILITEGARGEGGYLLNTLGERFMKRYAPEQMELATRSTVSL
                     AIGQEIQEGRGVDGCVLLDLRHLGRARILERLPQIRELALEFAGLDPIETPIPIRPGA
                     HYQMGGVKTNAWGETDLPGLFAAGECACVSVHGANRLGGNSLLETIVFGRRAGTRAAD
                     AIRDYDLQPVPEEVLKSEEQRLNTLLTNPGLERAWQIRDDLGKTMSLNLGIFRTKQSM
                     QEALGAIQALKARANRMCVQDKGRIFNTDLIQALELQCLLDIADTIVVSALGREESRG
                     AHYRADFPTRNDSAWIRHTVSHRREDGPQLSYAPVTITRFPPA"
     gene            54161..55474
                     /locus_tag="PK224_01830"
     CDS             54161..55474
                     /locus_tag="PK224_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRB14509.1"
                     /translation="MATVHPGTAISASAIPSPSDSTQAFVCPFCQTVTSCLPVAGIPL
                     HQYACHSCNENLLILNPDGQPADEDMVAPSSAGGSDATDMSRRLVGLAVALLACVLNY
                     ALLAGMASWMFNQIKSTHDPYDIDELTLSPIILKANGWTALHLAAARGDVSEVTSLLE
                     DGSPVDRHNNNGRTALFEAATRGQTPVVTVLLQYGANPNARMKQNVTALLTAAEHGHA
                     DTIAVLLSNGAEINALCTCGDSALHRAVRKGHLAAAQVLLEQGIAANQKSHGETALEI
                     AQHDEDRELIELLRAHGGREFSQAKAHRAQGVAFQKKGQVDKALFAYAEALNLDPDDP
                     EAYFDRGTALLEKDSPDEALIAFHAAIRLNSTFIEAYSAAASVHVARRQWDQASALWD
                     RYLAQRPQDGRAHFERAIVKRSKGDSKGFLQELQQACTFGYRAAC"
     gene            55478..56746
                     /locus_tag="PK224_01835"
     CDS             55478..56746
                     /locus_tag="PK224_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HRB14510.1"
                     /translation="MTTTDAQSETADHSPSVTCNRCFGRYRIRDARRLTGRSRSLCPT
                     CGARFAVVVAAPAVNQRQIEGQVPSPVLLHPPATDAPESTPSMQRGSFHGAGGTLLGM
                     QIVNVCLTIVTLGAYHFWAKAKIRRYLFSQTAFAGDRFAYHGTGKELYQGFLKATVVF
                     GIPYFSLGAAHSFLALPQSVDFLLQAMAALVLFLYVPVAIVNARRYRCTRTSWRGIRF
                     SFRGRTVDFLKLYFKGWLFTLLTLGTYYPYFQTQRQAFLHSHTYFGNQRFRFTGHGSG
                     LMVPFAVTLFTTYAVLCLCGLALALQLTNAGLTLLLIPFVLGPVWVWLLGQKQKYYWD
                     HTTFGEARFSSSITWQKLFGLYLGNLALLLLTLGWAWPWVTVRNARFFIGTLSLQGLT
                     DLDRVLQDTTETSVTGEGLSNLLDTGFDMD"
     gene            56752..57894
                     /locus_tag="PK224_01840"
     CDS             56752..57894
                     /locus_tag="PK224_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRB14511.1"
                     /translation="MPTSRSAHYLDGRTATRHGVTLTVAPATLHIVMPDGSTKQWPYD
                     EIRQTQGTYQGEPVRLEFGPEPAEAIVISTAALLDDIHEAAPALARHFHNPARRHARI
                     GWTLCAAVGVVLMVVGLYRWGIPGIASAATPYIPVAWEESLGRQVVAHLAPETQQCRD
                     PERLRKLDHIVQTLAATHPASPYRITLSVVDAPTVNAFAAPGGRLVILRGLLEQTASP
                     EQLAGVLAHELQHVYQRHTTKAILEQTAATLLLTAVSGDMSGGLAWGLEGARTMGSLH
                     YTRNHETEADIEGLRMMQAARLDPAAMIAFYGTMERGAQDHAGPPDFLSTHPDMGERL
                     ATLIALAGPSPSDAQRLLPGEDWKDIRTLCRLQAGGRSASASPELS"
     gene            57963..58634
                     /locus_tag="PK224_01845"
     CDS             57963..58634
                     /locus_tag="PK224_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB14512.1"
                     /translation="MRVLVIEDETKVGSFIKRALEEESYAVDLCEDGAQGLDMALSGS
                     YDLIMIDLMLPSLPGLEVLTRLRKEKIQTPVLILTAQSKVDQRVKGLDAGADDYLTKP
                     FAIDELLARVRALLRRGTAESSGTLQIDDLLLNPATREVTRGGQRIELTVKEYALLEY
                     FMRHAGRVLTRPMISDHVWNQDFDTFTNVIDVYVNYLRNKIDRGRARKLIHTIRGSGY
                     MLKVD"
     gene            58634..60037
                     /locus_tag="PK224_01850"
     CDS             58634..60037
                     /locus_tag="PK224_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14513.1"
                     /translation="MPLRVRLTLWYGTALALILLTFSVVLYTITARGLRDQVDESLQE
                     TASAAVRSLEKRGFLPLIDEDALLSQFPELTRIDKFFQIFSPTGTITIRSPNIRQHDV
                     PLSRHALEAAFTGSTVFESARYPNEPPLRLVSVPIIYRGSLLYIVQVGTTLEGVEETL
                     RRFLLVLVVMAPIALVVSLVGGWFLAGRALRPVDSITIAAQRIAAGDLTQRLTSERST
                     DEIGRLTDTFNNMIARLETSFAQIRQFSSDASHELRTPLTVMKGESELVLRRPRPVED
                     YIAVLESNLEEIDRMSRIVEELLFLSRADLGQVKTECEPVRLEALVEDIQRQACLLGQ
                     ERGVDVIMGTIQPATVRGDELRLRELILNIVDNAVKYSYPQGTVDIDLLVEGPTARLS
                     VRDHGIGIPQDAHKQIFDRFFRTDDARSHTKKGTGLGLAICAWIAEAHHGHIEVQSEV
                     GKGSTFTIVLPLAPLTA"
     gene            60152..61630
                     /locus_tag="PK224_01855"
     CDS             60152..61630
                     /locus_tag="PK224_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRB14514.1"
                     /translation="MKRPTQSVSAITGIAALSAALIWSYLPLTTSHASNPSPAAEERP
                     ATMTGTLPAAGFADVAKTVTPAVVNITTSGAEEVSDSSRPRGRMEDFFGSPFGPRRFG
                     PPMEPRERRGGGQGSGVIVTPDGYVLTNNHVIAGAKTVTVTLPDKREFKGRIIGSDPK
                     SDIAVVKIDGTQLPTVTWGDSSRLQVGEYVLAVGNPFGLNSTVTLGIVSALGRGHMGI
                     TQYEDFIQTDAAINPGNSGGALVNTRGELIGINTAIFSQTGGYQGVGFAVPTGMSKPI
                     YESLVKTGKVVRGYLGVGIQDLNQELAKSFDVKGSNGAIITDVKEEGPADKAGLKQGD
                     VILSFQGTPIEDAVTLQRAVTRSSVGSKATVKVMRDGREKDLTVTIAELPDNPQVAKA
                     EAGPSEQPLAGLAVQELDRETAQELGIKGKIQGVVVTTVDPESDAERAGLMPGDVIRE
                     INRKPVTSMKDFDRAASDLKKGQTVLVLVNRRGASLYLSAKI"
     gene            complement(61673..63127)
                     /gene="rimO"
                     /locus_tag="PK224_01860"
     CDS             complement(61673..63127)
                     /gene="rimO"
                     /locus_tag="PK224_01860"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRB14515.1"
                     /translation="MASSLITPSRAKRNKPTIGFVNLGCSKNQVDSEVMLGTLVAGGF
                     QLTGDARAAEVVIINTCGFIEEAKQESINSIIEHGRLKKSGSCRVLIAAGCLAQRYQG
                     ELLKELPELDGVVGTGEFGRIAEICRSLLAPKARQQRLWIGQPPYLYDAETPRIRLGT
                     PHSAYLKIAEGCNRNCAFCAIPIMRGKQRSRPIESIVAEAGRLVQEGVKELNLISQDT
                     INYGVDLGLKQGLTALLRSLVTVKDVRWIRPFYLYPQQVTDELLDLYAGEARITKYLD
                     MPLQHISDGMLKRMHRLGDRKHVTKLVERIRAKIPGVFFRTAFIVGFPGETDAMFEEL
                     KQFILDMEFDRVAVFLYSDEEGTSAVDLDRKVDQAVMEERRNELLALQESISEAKNRD
                     YLGRTMEVLVDGISEESDRLLECRHEGLAPEIDGVVFCDRSAAKPGEFVSVTVTDVAG
                     YDLIAQPVGRADAPTAAPAVPTLLMPKKSGAGYR"
     gene            63212..63751
                     /locus_tag="PK224_01865"
     CDS             63212..63751
                     /locus_tag="PK224_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRB14516.1"
                     /translation="MIRTFQGITPTVPQSCFIEETAVVIGDVVMGEECSAWFHAVIRG
                     DVNYIRIGHRTNVQDLCMLHVTHDTHPLIIGDDVTIGHNVVLHGCTIHNRVLVGMGAI
                     IMDGAVIGEDSVVGAGALITEDTVVPPKSLILGSPAKVKRPVTEQELAWIRESAQNYI
                     RYSRQYLSGPDKPRPGFWV"
     gene            complement(63791..64489)
                     /locus_tag="PK224_01870"
     CDS             complement(63791..64489)
                     /locus_tag="PK224_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14517.1"
                     /translation="MARKLMNVGTVARRAMKDIFARLDYGRLGPIYCDEGGDEFWAAK
                     RGLCQRLGLKLAKALLPRLQPGGTSLYVGAGVAEIPLLLMESLDLGRQVLACNLRKQE
                     VQVLNRACVGFPIRFRHQDAGSVKDRVDHLWLVSVLNDPERFPELSALSYGRANPVTF
                     DAKRFLPQQRIVATLVDRCLKRLIIPGLVTTTVEEAVWVAEWCHRRGVRYRIDQRTYA
                     SPTVGDPICFMQIG"
     gene            complement(64722..65618)
                     /gene="argB"
                     /locus_tag="PK224_01875"
     CDS             complement(64722..65618)
                     /gene="argB"
                     /locus_tag="PK224_01875"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRB14518.1"
                     /translation="MNKLIKKADVLIEALPYIRTFKGKTVVIKYGGHAMTDAPLKERF
                     AQNVVLLKYVGLNPVIVHGGGPQIDQMLDRLGMVAKFRHGVRVTDAATMEIVEMVLAG
                     RINMEIVDLLNRHGGQAVGLSGKDGGLMLTKPLTAKAWAESIEKDLDSDDSDADFGFV
                     GDVKSIDPTLLLKLQEDNYIPVIAPIGTDREGNTYNINADLVAGAIAAALKAEKLVML
                     TDVKGIRDANGRHLPTVSRKDVQRMVKRGTISEGMLPKVHACLDALAGGVGKAHIIDG
                     RTPHAILLEIFTHKGIGTEIVA"
     gene            complement(65644..67047)
                     /gene="hslU"
                     /locus_tag="PK224_01880"
     CDS             complement(65644..67047)
                     /gene="hslU"
                     /locus_tag="PK224_01880"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRB14519.1"
                     /translation="MTTESTSRPLNVNSLTPRQIVEALDRYVIGQHDAKRMVAIALRN
                     RWRRQQLAPELRDEVMPKNIIMIGPTGVGKTEIARRLAKLAEAPFIKVEASKFTEVGY
                     VGRDVESIIRDLTELAISLVKTKHLEEVQGKASRLGEERLLDLLLPGAPSRPTSPGFE
                     PSGARADASEPTESPDATRSKLRLQLREGKLDQRSVEVEVKERALPLGVISNAGGMED
                     LEGNLRDMLGGMFQGKKKKRMMKVPDALKHLTQEEAQKLIDMDEVVREAITKVEQTGI
                     VFLDEIDKIAGRERAMGPDVSREGVQRDLLPIVEGSTVSTKHGAVQTDHILFIAAGAF
                     HVAKPSDLIPELQGRFPIRVELAPLTKDDFVRILTEPRGALVRQYQALMATEGLTVEF
                     TDDGLAEIAATAVQVNERTENIGARRLFTIMERLLEQVSFEGSEMNEKTIVVDAGYVR
                     ERLQNIVKDQDLSRYIL"
     gene            complement(67060..67596)
                     /gene="hslV"
                     /locus_tag="PK224_01885"
     CDS             complement(67060..67596)
                     /gene="hslV"
                     /locus_tag="PK224_01885"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRB14520.1"
                     /translation="MIIRSTTVLCVRRDSQVTMGCDGQVTVGTTVMKHNARKMRRMHG
                     DTVLAGFAGATADAFTLFEKFEAKLAEYRGNLTRAAVELAKDWRTDRVLRRLEALLAV
                     ADLDHSFIISGTGDVVEPEDGILAIGSGGPYALAAARALLGHSELVAEQVVRESLMIA
                     GGIDIYTNQQIVIESLSR"
     gene            complement(67593..68549)
                     /locus_tag="PK224_01890"
     CDS             complement(67593..68549)
                     /locus_tag="PK224_01890"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRB14521.1"
                     /translation="MDRAIRTFLDVLAVQQNASPQTIRAYASDLAQFQAFALGVQKPG
                     GPLVPESVTPALIREFLAARDRTGEKKTSLARKLACLRSFFRYLVRVGQVEVNPAEDV
                     RAPKLPKHLPQVLTKDDAGALMEFPGGTEREGLRDRAILETLYSTGARVSELVGMNCD
                     DISRSEGLVRVRGKGRKERVIPLGSVALEAIDAYHAQVQTVPGPSSGRSSDAVAVFRN
                     SRGGRLTTRTIARIVAKYSRQLTGGAVHPHTLRHSFATHLLDEGADLRAIQEMLGHAS
                     LSTTQKYTHLATDQLLALYDRTHPRAVRTTEASEVIKQKGSR"
     gene            complement(68522..69838)
                     /gene="trmFO"
                     /locus_tag="PK224_01895"
     CDS             complement(68522..69838)
                     /gene="trmFO"
                     /locus_tag="PK224_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRB14522.1"
                     /translation="MREDIVIIGGGLAGTEAAWQAANRGAKVTLYEMRPKEMTKAHKT
                     GDLAELVCSNSLGSADPLNAPGILKTEMRRLNSLVIRAAEEARVPAGSALAVDREQFA
                     QVITQALEGHPNIRIMREEVTEIPQDAVCIIATGPLTSEKLSKAISELTHERHLYFFD
                     AISPIIDAESINMDIVYRASRYGKGGADYLNCPLDEAAYNALYDAMMTAEKVQPKEFE
                     KIAYFESCIPIEVMAERGRQTMQFGPLKPVGLENPKTGVRPYAVVQLRTENAHGTCYN
                     MVGFQTKLTYPEQRRVFRLIPGLENAEFLRLGSLHRNTFINSPQLLRETLQLKSRGTV
                     FFAGQLVGVEGYTESAAMGGIAGINAARGLADQPLVTPPPNSAHGCLIAHITKSDPAH
                     FQPMNTNFGLFPPVTVKTRDKDQKRRLIQQRAVEDFDAWIAQSGLS"
     gene            70037..70111
                     /locus_tag="PK224_01900"
     tRNA            70037..70111
                     /locus_tag="PK224_01900"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:70069..70071,aa:Val,seq:cac)
     gene            complement(70068..71000)
                     /locus_tag="PK224_01905"
     CDS             complement(70068..71000)
                     /locus_tag="PK224_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB14523.1"
                     /translation="MDRFEREHLVKLASRQTGQAFVKRFRAFFGDRTLGEITPKLIVE
                     YKSRRYAAGVKPASINRELTCLRKAFNLAKREWEWCRDNPVSRVSLEKGATKRDRWLM
                     EDEEARLLDACPSWLRELVVFALHSGMRLGEILSLTWAGVDLFRRTATVFESKNGERR
                     TVPLNQTLMALLTEKAKVRHIKTALVFPSLAGTRLDPNHLRRALRPAMAKAGIVNCHF
                     HDLRHTFATRLVQSGVDLYKVQRLLGHKSPMMTQRYAHHYPESLRDGVEILDRRSHRD
                     TKMTTVLRVSESAVLEVVEKLVGDTGIEPVASSV"
     gene            complement(71176..71490)
                     /locus_tag="PK224_01910"
     CDS             complement(71176..71490)
                     /locus_tag="PK224_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB14524.1"
                     /translation="MLTVKELSVWLNIKQSTLYLWVSKNKIPCRRIHGLVRFEPEAIQ
                     AWLNGFSSTSSNLPPRVPRHKAGDVDHLIEAAKRAVYTPRHGETRPTASPLGKESNDG
                     AR"
     gene            complement(71533..72540)
                     /locus_tag="PK224_01915"
     CDS             complement(71533..72540)
                     /locus_tag="PK224_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB14525.1"
                     /translation="MDSSFTLTIDWLAFTVLASNPQETMKVLGGDWSKAKGGFRGYPL
                     SWMRVDGLRGVGKLGTNAPRRPNEIHVDLSGGLASALTLDQIRTLLQWVHKQQGHVTR
                     IDCALDDRAGTVPVRTIREAVAAGQCVTRAAQVRHIVSNLTHGTGATTGETMYFGSPQ
                     SQTLLRIYDKRLELQSKGQENWPEYGTRWELELKKDRAEQCARALATLDETDWKELVI
                     GLLRSYVDFRQTSKDAEDEERYRAPVLEWYALLTEGFQKGRLAQEQQVQTLQNVKRWV
                     SDTLTPMLAVICATPGGEEWLLNEIVRGIARWNDRHRNLLKQPPTRFHRSAGGHAGSP
                     C"
     gene            complement(73178..74056)
                     /gene="lepB"
                     /locus_tag="PK224_01920"
     CDS             complement(73178..74056)
                     /gene="lepB"
                     /locus_tag="PK224_01920"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRB14526.1"
                     /translation="MPPARPQAERKPWIAGLLNILLPGFGHVYLGRTKQGVYWFVTFQ
                     LLALASVLIARSPLLPTGLNLAGILVPLIGYIFLVRSAVRVARRRQQTGLPESLPSKQ
                     FSLAVFVIGGCLAWFMPDLMRGIVKAHKIPAESMAPTVLADDYIYVDTLISHIKFEPR
                     RGEIITFLYPKDEGKSFLKRVIGLPGDRIEIRNKIVFVNGVPLDDGSYTQRLDPRVVP
                     GHEHPRDNFGPVIVPEQFYFVLGDNRDASLDSRFWGYVHESKITGRARLIYWSVNKQG
                     AWSEWVRWERIGKRIQ"
     gene            complement(74304..74723)
                     /locus_tag="PK224_01925"
     CDS             complement(74304..74723)
                     /locus_tag="PK224_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRB14527.1"
                     /translation="MRVVLDTNIFVSGLLSAAGPPARIIQAVLQRRLIPVMSSDTFAE
                     LEAVLRRPKLQRAFTRAGVNITSFLETIQAEVQIVDPHPTNTPLRDAHDRPFLDLMAT
                     IPPPQYFVTGDKDFEASHYSGVPVISAAEFARLLAHR"
     gene            complement(74707..74961)
                     /locus_tag="PK224_01930"
     CDS             complement(74707..74961)
                     /locus_tag="PK224_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14528.1"
                     /translation="MKAVKYTKEGVVIPSSWMKGWGKAVVAHRDSDILILESPARAAS
                     RKKLVRMVSKLRRATRELGVTPEQIAAEVAAVRRERARRS"
     gene            75514..75843
                     /locus_tag="PK224_01935"
     CDS             75514..75843
                     /locus_tag="PK224_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14529.1"
                     /translation="MRGTVATIILGLVLTTGCSTTNQVATQDQNYNAPLSIYSAMDST
                     SLVYSDPMAASQANDHPYRWAAFILHPVGHLVDYTVNRPLYAIAKHAPYLFGYTSEDS
                     MIDSQRR"
     gene            75994..77139
                     /locus_tag="PK224_01940"
     CDS             75994..77139
                     /locus_tag="PK224_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14530.1"
                     /translation="MYRQLSLLPFLFAACALWAGCETPPPARPPLPPSESDLDLLGPT
                     KLCDSRQTFLAAHPGITPHTHAWGSGQELRIPSEHSRSKSEESYFFDEDGTLVGMLFV
                     FRSGLDLAPYKTLRYTLSRLKPSLEFYLTVAQLADRQNMEASTIYDTGDEKTTTRYLV
                     LGDRSDQRLLEASFTVDPYVKLFSPYRKEFLDRLRDTAQHTGGQHLDTQGSEDKEPFA
                     SLQQFARGQTAQLAYCGTKNQVIALDAYQKASASGFSGTVWQAELRHRLGVSWEAAGN
                     LEKAKSELLASLALRPNSPEVVNNLGAVYWKLGEKKNALASFEKAILMRPNYAIARFN
                     VAEAIADDNPRRAITEYETYLALVEGILEETDRAALAQKRVQALKHQ"
     gene            complement(77207..77614)
                     /locus_tag="PK224_01945"
     CDS             complement(77207..77614)
                     /locus_tag="PK224_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRB14531.1"
                     /translation="MKTPAKKTTVARRKPPKANGVKYPDILADLEGQRAAILAEAGVV
                     LTNPTGLEVFPDVSDQASAEADQHFSFRIRERERKLLKKIDEALGRFATQTYGICEGC
                     EGDIPYKRLKARPVTTLCIECKTSQEEAEKSPR"
     gene            complement(77755..78357)
                     /gene="recR"
                     /locus_tag="PK224_01950"
     CDS             complement(77755..78357)
                     /gene="recR"
                     /locus_tag="PK224_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRB14532.1"
                     /translation="MSVDQQGLLAKLVRELVRLPGIGQKSAQRLAFHLLKAEREDAMR
                     LAEAIQAVKDGLSFCRQCRNIAEGELCEFCRDPKRDRSKILVIEEPSTLYAIERAGGY
                     RGLYHVLLGVLSPLDGVGPSDIRAEELLDRVKAGGVEEVIVATNPTIEGEATAIYLTR
                     LLKPHHVRVTRIAYGIPVGMDIEYADEVTLVKSIEGRRDL"
     gene            complement(78394..78717)
                     /locus_tag="PK224_01955"
     CDS             complement(78394..78717)
                     /locus_tag="PK224_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRB14533.1"
                     /translation="MKSPFGNMSNILKQAQAMQEQMAKVQEQAATKTVSGTAGGGIVT
                     VTVNGAMDLLSVKIDPEVVKAGDVEMLQDLVVAAGNDALKKSREMMAEEMKAVTGGMK
                     IPGLF"
     gene            complement(78714..80591)
                     /gene="dnaX"
                     /locus_tag="PK224_01960"
     CDS             complement(78714..80591)
                     /gene="dnaX"
                     /locus_tag="PK224_01960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRB14534.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQSHVVQTLMNSIATKRIAHAFLFS
                     GTRGVGKTTVARILAKALNCEQGPTGTPCNTCSNCQEITQGTSVDVVEIDGASNTSVD
                     DVREIRENVKFTPFRGQYRVYIIDEVHMLSNSAFNALLKTLEEPPSHVVFIFATTEIH
                     KIPATILSRCQHYNFRRISKAEIVQRLRHVADQDGLTIEDRSLMALARASEGSMRDGL
                     SLLDQIIAFGGNTIRHEDLEALLGAVPQERVRAMVEAVIQQDSAKALQVIAALLDQGH
                     DVRAYCADLVEYVRNMLVAAVVPSGPELRSLIEATEEDLTQLARDAERFTVEQLQELF
                     RMYAAAEDSLRVSAHPRFALETAAVRATRLLRTAEVQPASSRLAVQPEKPAADRRIVT
                     QTPAQTQDKATPSPAPVVKTTGVKVSQDNVAKTSAAGGSTPKAPSVAPPREVAAPPAR
                     TSAVAPSAPVVPPASAAPVQQEPKVTSAAEASAVAVEVNWEQFQEAVSTNHPNIAPFL
                     EMGRLVKIEGGLITLGFAKQATTARSMLEKEDNLQAMAALGESLYGCAVRIRIVEVAE
                     QDPGTAPTMKQIRVAKEQEQRLILTQQAKAHPLVKQALEMFGGELAEVRTTAPAQEVQ
                     E"
     gene            complement(80673..80772)
                     /gene="ffs"
                     /locus_tag="PK224_01965"
     ncRNA           complement(80673..80772)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK224_01965"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(81023..81112)
                     /locus_tag="PK224_01970"
     tRNA            complement(81023..81112)
                     /locus_tag="PK224_01970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(81076..81078),aa:Ser,seq:gga)
     gene            81331..83439
                     /locus_tag="PK224_01975"
     CDS             81331..83439
                     /locus_tag="PK224_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRB14535.1"
                     /translation="MKILVGIGVVILLLIVLIVALPFLIDLNKYQDRYRPLIEEALNR
                     KVELQDIRLTIWPRIGARVGNFVVQDDPAFRTGPFASLSSLDVGVKLLPLLKGKVDVE
                     EIALRDPVITVLKNAQGQLNISTLGAKTPAPPAPSKPEAPAQAPGSPLQILALFAVDR
                     VSIDGGKLSYRDESTPKPTEYTVNNLEFLLTSVHLDESPTVHLGARVQPYNLPVRLDG
                     TFGPLVETLDVKSFTFNLALGKVAVGLKGRAVGGNLDATVNALQIDTADLPIALPLTK
                     PVQLKDVHLTLHALYPIPPDTPPANQVDLTDLGLTLVMGGSAINVKGTATKGLANLTA
                     ASASINSADLPVAIPLTKPVELKDLHVNLRAKYPPKEGAAPLELAEIPNLGLTVAMGS
                     SRIEVKGSVLGGLAKITANSKLINTTDLPIALTLKKPVEVKDLQAAAEMKGQEARLTN
                     ASLQLFGGFVRTQGTLGLGSATPPFSGTVSIQGLQLGPALQAIGTAQVSISGTANAEL
                     AVNGRGFTHPDLVKALAGTGRAAVKEGKIEGINLLQQASMLLKVVGVSLDNVKATAFS
                     TIESDFAIKQGLIAVQRLLIDSHDFQATGGGTIGLDQSLDMKLNLNLSQALSQKIAAG
                     SPIARVALTGGRLSLPLLITGSTQAPSYGLDTKMFAGKVKEQVKEKVKGAVGDLLKGS
                     AKPDDLKQQGKDLLKGLFGR"
     gene            83458..84258
                     /locus_tag="PK224_01980"
     CDS             83458..84258
                     /locus_tag="PK224_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRB14536.1"
                     /translation="MSLANLVTQYIVQYGFRIMGAILVVVAALVVAKSVGRLFERWLN
                     EQDLEPPLRLLLLRLVKALVVILGLLIALDQLGLQIAPLVAGLGVAGLGMGLALQGVL
                     SNVVAGLSIIFTKPYRVGEHVSLLGVHGDVAKIDIFTTTLMHPDHSRIVIPNRKVVGE
                     ILHNFGTIRQLDLSIGVSYQTDIDTTLRVLRDLVTRHPKVLQDPAPMIGIAGFADSSI
                     TLSIRPWVQVAAVGTAHSELNQAIIQRLQADGVDIPFPQREVRLLNPS"
     gene            84391..85008
                     /locus_tag="PK224_01985"
     CDS             84391..85008
                     /locus_tag="PK224_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14537.1"
                     /translation="MSRQTDLDRPRRSERTSGSSLADTETESVLTEEHAAGTNLDKIR
                     DILFGAQVRDHERRFAKLEAQLLAEAAQLRNDLKERFASLEQYIRKEVDTVTGRLTAE
                     EQDRTDAVGRLTTELQTLAGVLQQTATQLREQSEQAHHDLRTQLQNQATSLSGDFTQQ
                     HAALSATLDDAVRQLSHQKTDRASLATLFQELSQRLSHDQPPQQA"
     gene            85125..86855
                     /locus_tag="PK224_01990"
     CDS             85125..86855
                     /locus_tag="PK224_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB14538.1"
                     /translation="MAHAPKAGTPAVERDYAELRSLLLAPEQSRLEQLQEQVEHLDLN
                     AHNLNRVLPEAIALRGETDHRLTHALTPHVSEALGVSVRKQPHMIVDAIAPIMMPAIR
                     QAIANALRSMVQSLNQTIEHSLSIRSMQWRLEALRTGKPFAEIVLLHTLCYRVEQVFL
                     IHAQTGLLLAHAAGDAVAVQDQTLVSGMLSAIRSFVQDSFGATPDQALNTLQVGDLTV
                     WIEQGPSAILAAVIRGTPPETFHVHLQDTLDRIHAEHSDALTRFIGDAAPFTGTTPLL
                     EECLRAQFETRRRAIAPITWVLLAAIVLAAIWWGVSAFQDRQHWQAYLDRLASEPGLV
                     VTSTHSAGNRYVLTGLRDPLAADPDVLLQDSGVAADRVEAKWSPYYALDAAFTLKRAG
                     IVLSPPDTVRLTLEGPRLTATGTASAEWIRQSRPLARLLPGITEYDDRRLVAQSLEAL
                     VQRMAGVWILFQQGTATIQSPEQLQGVRRISELLHQLDDTAHLSGATVILEITGQTDV
                     VGRSSRNQRLSEDRARSVLDALHPATFSAITFHARGIGPAPESSPSLGAATLPQDRRV
                     SFQATVHPAS"
     gene            86869..87405
                     /locus_tag="PK224_01995"
     CDS             86869..87405
                     /locus_tag="PK224_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HRB14539.1"
                     /translation="MIEKKICMLGAFAVGKTSLVRRFVTSCFSEQYQTTIGVTVDKKT
                     MSMDGQPVTLVLWDLYGEDEFQKLRRSYLRGSSAYLVVLDGMRRATLDIALHIQLTAA
                     DTLGPVPFVVLINKLDRQRDWEVTDQDLAQLAQRGWTVLLTSAKTGQGVEEAFTVLAR
                     AMLATSEQTPPAGTTDGA"
     gene            87395..89191
                     /locus_tag="PK224_02000"
     CDS             87395..89191
                     /locus_tag="PK224_02000"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB14540.1"
                     /translation="MAHNTHPSAPDLLCTDLLQRLQFAILEHVTGTEFRIIGHPPAWL
                     FTLYPDARHNRPLSVDARTPVLQNFLADAADAWQTDGDDIAHSGFWSEQTAAEEPWHF
                     HAMALRHGPSRLLLIQQSTAAYDQQATLLQRARDQVLQQHEHNRGHQRTQHELTTKLV
                     DMERSRDDVAVILQQLGLATLLIDQQGQVRFLSASAARLLDTPATGGPNGLLWETLLP
                     LTKPDRLALQSMLQQPAPRRERVQCHVETQAGRHLWLEIELQDDPRDTGTKIMFLHDM
                     TDVHHLRRLLELKAHYHDLVGKSRGMTQIYEQIQDLARVDSTVLIEGETGTGKELVAR
                     ALHQASARHKGPFIAANCAGLTDSLLGSQLFGHKKGAFTGAIDDQQGLFEAAQGGVLF
                     LDEIGDIPHNVQTNLLRVLQEKEVTRLGETRPRKVNVRVLTATHHNLSQDVAKGTFRA
                     DLLYRIRVSRIQLPPLRERKEDIPLLVASFLTEGRASMGKVIHRASPAAMAALMEYHW
                     PGNVRELKSTIECAMIHCKGDMLEATDLPPELRQGPTDHPSEVVLHGDERSRFVAALG
                     QARGNRTKAARLLGMSRATFYRRLSELDLPTS"
     gene            89459..89776
                     /locus_tag="PK224_02005"
     CDS             89459..89776
                     /locus_tag="PK224_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB14541.1"
                     /translation="MDKLMPFSRPQPDAPTAREREILNHIWAGLTSQEIATRLQIAPK
                     TVESHRANLLRKFRATNSAQLLRLALLEGILHMPSSTSTGPSQTAADGQSTTAVRKLT
                     NLK"
     gene            90153..91253
                     /locus_tag="PK224_02010"
     CDS             90153..91253
                     /locus_tag="PK224_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRB14542.1"
                     /translation="MRRLGTLCFALLVMALAASIARAEAPAPADATPALDAVTLKDGT
                     VIYGQVLGMIADELHIKTSFGPTAGDDIVKIMWPNVAKLVVNRPVPFSLKEGTTVVGT
                     AQPGEPGTMILKAAPTGTPMAIPLDAVIGMNQPAVIYTGALQAGFSQTTGNSHLRNGS
                     LLGELSARSESLRLTILGRYIYGDNAQNLIVRNSRGTIKLDFFLSKRLYWFASAYFEQ
                     DTFQDLKLRTALATGPGYQLLDRGDLTGFFKDMTLWAEAGAAYFNEDFKLADDKSSTR
                     GRWAVKWNWPLWGGDQVSLYHFQEGFQSLANAKDLYLTADTGLRFKVWGGLVSGFQWT
                     MRYNKNPPPGVSDTDNLYLITLGYSFDTSRKQ"
     gene            91304..91729
                     /gene="mscL"
                     /locus_tag="PK224_02015"
     CDS             91304..91729
                     /gene="mscL"
                     /locus_tag="PK224_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRB14543.1"
                     /translation="MSMMSEFKEFAVKGNVLDMAVGVIIGGAFGKIVSSVVSDILMPP
                     IGLLMGKVDFSSLFIPLTEDAKGKSLAAAKAAGAATINYGVFLQTLLDFTILAFVIFM
                     VIKQMNRFKKTAPPGPPPAPPKEEALLTEIRDLLKNQRR"
     gene            91805..93007
                     /locus_tag="PK224_02020"
     CDS             91805..93007
                     /locus_tag="PK224_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB14544.1"
                     /translation="MTTLRTIPVLLSTLLLASCATAPSPNDSASRALNEMKAVAKPLP
                     PKPDPKDARIAELERQKAALEAELAQVRSSLTGDLDQAKARTSALESQLSQRDQELAS
                     LRNASGDKERLASQLSDVERQLSAKDQELAALRGSAGDKDRLAAQLAALQGQLSTKDQ
                     ELAGLKGNAGDRDRLSSELAQAKQRITELERQLEAKHHELAASKNAAGDREKIVADLA
                     AAKQRASDLESELARRDQEMASLKGALDQQKTSLAEAKDDLSKLLQAEVAKGNVTMKQ
                     LGDQLTLGLATTLLFDSGEATLKPGGADVLHRIGGVLKQYPDRSIHVAGHTDNVPIKG
                     RLAKTYPTNVELSQARAESARQALTEGGMAPDKIEAKGRADSRPIASNSTAEGRQKNR
                     RVEIVVGH"
     gene            93253..94758
                     /locus_tag="PK224_02025"
     CDS             93253..94758
                     /locus_tag="PK224_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB14545.1"
                     /translation="MRRAVWALMVLSSLAAGCETAPHVQPIASSPATVEPKLLALQQE
                     REQLLTTLGEFHDRIRDLESKLGDRQSQPAAASYDQLLSAKEADLVELRRLAPERDRL
                     TVQLTTAANELIQARQRIGTLEQQLATRDKDLAALQTRATAVADLEVARRRISELEAH
                     IARQDRDLHTVRTGSAERDSLAAQLQTATATIDSLKARISALDQQLKDREQAYETVRS
                     RLMERDKLVPQYNAMIAEIYQARHRIAALEQRLNEKGRDLSPRQKGTQLTTSQRDGGP
                     LNQRSGAANQSLGDTAKSIPDGKSAPQGKDSTRSGVAFPGNPAQAEARSGSFAAVKEE
                     LLKVLPGDNRQKTISMRQDGNRLTVALDSNLLFTSGDAALSPEGATILKRIGAVLGQL
                     SDKFVQVAGHTDNQALSKTLQKTFPDNKALSWARAENARRALVNGGIPADRTKAVGLA
                     DTRPLTSNATEQGRQKNRRLELIIVQGPTVAATVEESRRGQVRLATLSATP"
     gene            complement(94823..96067)
                     /locus_tag="PK224_02030"
     CDS             complement(94823..96067)
                     /locus_tag="PK224_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB14546.1"
                     /translation="MNRISRRRFLQLSAMTGGALLFSDLADRVLGASSDHAVAFAAEP
                     IKIGILDPLSSPYKTSSIHDVHGANVAVDLFNKRGGVLGRPVVILEADDASNPETAIK
                     AATKFVQDDQVDVLMGTFNADCALVVSELAKKENKLFMVTGSLLPELTGAACSSHTFV
                     FMPNASMMAQAVVPHLVKAYGTRWYMLTTSSLDGKAMAQAMVTAGQAHGVEFVGETLV
                     PFGATDFTAAFTAAKDKHPTLVVLNLYGWDLVHALKAYTKLEFAKEKIGVGGMIAGEQ
                     IGRPLGYANNAGIWGLIWDPKVNTEGSRQFIRAVVDKYNHTPTSRCYLGYAAMTQVLE
                     AIQRAGTTGAAALIKALEGLEFDGLKEGRSYFQASNHQHMQDVLVGEAYGKELGLGHY
                     KILATVPADRLAAPVHAGVCQL"
     gene            complement(96422..97507)
                     /locus_tag="PK224_02035"
     CDS             complement(96422..97507)
                     /locus_tag="PK224_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB14547.1"
                     /translation="MTRSASFAAAIFLLLLLGIGSTAWAYRDYFSETQKAQLANIQTV
                     LVEVIALTDTGAGNPEGIREVVTRRMKEMGYAAIADPALPHDVVVRVKCEQRKTWEGT
                     AAAGGDNDLLDAPSRLWKGPACQVTYALGGMKIKWQKEVRTEFEDANQAAQSAQGGAP
                     GPYALTALQVELEKYEFPLFLTAEWGQPDRLLKLMDSPDTSQLRKLKIISLLGEMVAD
                     EALPHLTEALKDKDLAKQAAVALGNMGKEGIPVLIEILKHSKQPDLQAAAAKGLGDLG
                     NTHSDPRVVPPLLEMLDAPGIDIAVQTEIAWALGRVPDRRSVEPLFALDRKLQKIRND
                     PPDPQIKKLKEAVFWSIKQVYTEDQYS"
     gene            97835..97917
                     /locus_tag="PK224_02040"
     tRNA            97835..97917
                     /locus_tag="PK224_02040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:97869..97871,aa:Leu,seq:tag)
     gene            97990..99303
                     /gene="tig"
                     /locus_tag="PK224_02045"
     CDS             97990..99303
                     /gene="tig"
                     /locus_tag="PK224_02045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRB14548.1"
                     /translation="MTELGPMKRALKIEVPADEVNLRFVQAYSELNRQVRIPGFRPGK
                     APLQLLEKRYAKTIEEDVIRSLVPDYYDRAIRQAGIVPVLVEIPPLERVKVKKDTPFS
                     FTATVEIKPTIELRDYKAPNPISLKQDQRTVSDEQVHKALDVLREQMAQLHPAPAGTT
                     LADGDYAVLDIEGTLDGKPLEGTTKEGHLHKMGSHASVLGIDIESHLPGKKEGEAITI
                     PQAYPATHPDTRVAGKTVSFRCTIKSVKQKQLPALDDEFAKDCGPYQSLQEIREKLRT
                     EMERALKKDIEDSYKDTILKRLAETHHFELPGTLVERELSAMVRQQLQSRQRKSGDNP
                     DTSPTAPQTEDAKKLQDELRPEAERRVKVGLILEALAAKESITVTNEDLGNEITRLAA
                     EVKLSVEEVTRMIQAGGQDTLDDLRSRILADKALDFVYKHAMIQG"
     gene            99430..100065
                     /gene="clpP"
                     /locus_tag="PK224_02050"
     CDS             99430..100065
                     /gene="clpP"
                     /locus_tag="PK224_02050"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRB14549.1"
                     /translation="MLVPIVIEQTNRGERAYDIYSRLLKDRIIFLGAPIDDVFANLII
                     AQLLFLEAEDPEKDINLYINSPGGSVTAGLGIYDTMQYVKPAINTICLGQAASMGAFL
                     LTAGTKGKRYALPNARVMIHQPMGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     LDKISQDTERDYFMSSEEAKRYGLIDEVITRPLKSLKPVGSTDSGKDGGKS"
     gene            100092..101348
                     /gene="clpX"
                     /locus_tag="PK224_02055"
     CDS             100092..101348
                     /gene="clpX"
                     /locus_tag="PK224_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRB14550.1"
                     /translation="MAKQEKIDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPAEIKHHLDQYVVGQDRAKRILSVAVHNHYKRISAKEKD
                     VDDVELQKGNILVIGPTGTGKTLLAQTLAKFLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQAADYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTSNILFVCGGAFVGLEHIIEQRMNRKSMGFGAEVRGRND
                     IRLGELLPHVQPEDLLKYGLIPEFIGRLPVVATLDELDEQALIRILTEPRNALTKQYE
                     KLLSFEKVKLRFTEGALSAVARKAFTQKTGARGLRSILEEAMLDVMYDAPSQKQIKEV
                     VITEDAISGKHAPIRIFEQDKDVKSA"
     gene            101565..102089
                     /locus_tag="PK224_02060"
     CDS             101565..102089
                     /locus_tag="PK224_02060"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB14551.1"
                     /translation="MKFPVVSSNRHKGKTLEIDSSKEVITLVGINGEPMGSLAWDFLV
                     DQILTYRKPQQSREARSEPRISLSIRVKYHTPEGTQFESRAGGIGGGGLFIESFTPLA
                     VGTKLAMEFTLPESPGEWLSAKGVIAWVCPKADQYTFSPGMGVRFSDIAAETRNRVLS
                     LVHSVKSITQDEPK"
     gene            102155..102673
                     /locus_tag="PK224_02065"
     CDS             102155..102673
                     /locus_tag="PK224_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB14552.1"
                     /translation="MKFPVTATREHQGKTLSIDCDQETLSLFDDTGQRLSILTWGTLI
                     EHMLSVDEDARFAHSRAHPRAPLAIKVHCTTGDGKHFDSLTGGISGGGLFIESSAPLA
                     PGTELKVEFALPDRPSQKFETRAKVAWARSKPERYLLFPGMGIQFTDIDPEAQARLID
                     LVASLNRTRATA"
     gene            complement(102627..103418)
                     /locus_tag="PK224_02070"
     CDS             complement(102627..103418)
                     /locus_tag="PK224_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB14553.1"
                     /translation="MRSYFRLQFFFWLLLCSVVYGCYVDTPSGAPETVSRRLVELLAD
                     PDSDVRRTAAEALGKVGHKSSNASLIVALNDPDARVREAAALALGRLGDGKSGMALAG
                     HLADFAEPVRMASALALGEIEFSADREAQTLAVLRHPQGSARIAATRALLSLDTVSLS
                     ADLIKALRDPDARVRQGVAAVLGESGNPGAVSHLRSLLRTDGAASVRAEAAFRLGKIG
                     DNGVLAELSRAAEADPDMMVRGWARWAVQQITLSHEFGSERQPSQ"
     gene            complement(103663..104514)
                     /gene="tatA"
                     /locus_tag="PK224_02075"
     CDS             complement(103663..104514)
                     /gene="tatA"
                     /locus_tag="PK224_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB14554.1"
                     /translation="MFGTMGFSELIIILVIVLIIFGAGKLPQIGEGVGKALKGFKKEV
                     NDIPPPENAPDQNETAAMPAQIQAQPAADIPQVAQPATVAPAAAPKATSPYTPGPELT
                     PGTTAALMAAAGPQGPQATQPTKPRVATVTPGQAAAGSAVAQSHQPPTMEERMAAPAP
                     VMRAQYPPLPPGAQSKPVAKRPSAIVNKDAVARVQAAQAAMRTKAAQAQPSGLSAQDM
                     QGLGEGLGDAVRTFRQAVADVRSSVDPEMRTIRAEMDSAQKELEQSIEAAKQAPVLDD
                     DASAKSV"
     gene            complement(104661..105878)
                     /locus_tag="PK224_02080"
     CDS             complement(104661..105878)
                     /locus_tag="PK224_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRB14555.1"
                     /translation="MNVADRSHARVIRIQQAIREQPGLDGWLFYDFRHLDPIAYRVLL
                     LDPSLHVTRRWYYWVPAQGTPVKLQHRIEPHVLDGLPGDAHAYVSWRDQQAALGSLLH
                     PAKRIAMQYSPMNAIPYLSRVDAGTIDLVRSLGAEVVTSADLVQQFEAVWDDAQLASH
                     QVAAEGLRAIVDEAFGFVGTSLAARSSLTEYGLQQYILSRMQARGLVTSSPPIAAVNA
                     HSADPHYAPASEGSAPIRQGDLVLIDLWAKQPGPGAVYADITWTGFVGATVPARHQDI
                     FQIVRRARDAAVTFVQGRVRAGECPYGWEVDDVCRRVIQDAGYGQYFVHRTGHSIGEE
                     VHGNGANIDNLETQDARRLLPGTCFSIEPGIYLPDEFGIRSELDVYLSARDAVVYGQP
                     VQADLLPIPIPAS"
     gene            105973..106431
                     /locus_tag="PK224_02085"
     CDS             105973..106431
                     /locus_tag="PK224_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14556.1"
                     /translation="MAIVPSSDPAQYRVTFFFGPEAVEERPDHLRCVFNVKKRSWKGG
                     VQVAVDVARRHLDRARERIGYASWLAELIHSGHQEEREELTSRADDLFVQSLCTSALN
                     LALQAGIEQVNQQIDAATFADELHQLLDEQPEQVTDRIRLELDLGETSEI"
     gene            106565..107038
                     /locus_tag="PK224_02090"
     CDS             106565..107038
                     /locus_tag="PK224_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14557.1"
                     /translation="MNVSVYSPLKRRRSLWAQVLVFSLVTVCSLALLIAPVSAQESGG
                     ASNVPVSSNDASPSGVGIQAGSALATIVYFPLKLAFAIGGGVVGGLAYGFSGGSEQTA
                     KNIWIPSMYGTYIITPDHLKGDKPIRFLGVSGEPAREAGDYAPQEPAPLPLEPIR"
     gene            107040..107816
                     /locus_tag="PK224_02095"
     CDS             107040..107816
                     /locus_tag="PK224_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRB14558.1"
                     /translation="MKPVIGVTPDFNAGDRQEWGGKEPTYFLRARYVRAIEELGGVPV
                     ILPLVANRSARRRLLEGVDGLLLTGSGPDLDPTLYGEKKRYTFPVVAKRRSSFELDLV
                     RLAIRNHIPTLAICGGMQSMNVACGGSLYQDIPAQVDHVLQHRQTTPAVRLSHSISIT
                     PGSLLNRIVKRARMQVNSSHHQSVKAVGHALIASATAPDGIVEAIELPTHPFFLGLQW
                     HPEFLFERHLLHRRLFQTFLRAASRRPSLQPSPARRRNNS"
     gene            107880..109325
                     /locus_tag="PK224_02100"
     CDS             107880..109325
                     /locus_tag="PK224_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRB14559.1"
                     /translation="MGNPLFRTKSIERILADSDAPEHRLKRTLTAWDLTGLGIGAIIG
                     TGIFVLIGTAIVGDAHRPGAGPGIMLSFILSGITCALAALCYAEFAAMIPVAGSAYTY
                     SYATLGEFLAWLTGWNLILEYGVACVAVAIGWSGYFNNILKLCGLELPYWATHPPGAD
                     GGIANIPAAIIVLLVTCILIVGVKESARATCGIVLIKLAVIAFFIAVGTSSVDTANWS
                     PFMPFGFAGVGAAAAIVFFAYIGFDAVSTTAEEAKNPQRDLPIGIFASLAICTVLYIS
                     VAAILTGLVPYSQIDVHAPVAEGLRMVGFKWGAAIVATGAVAGITSVLVVMMLGQIRV
                     FFAMSRDGLLGPWLSGVHPKFRTPHHATYLTGVAVAIMAALIPIGEAADMTNIGTLFA
                     FVLVCIGIMVLRYTKPNHPRPFRMPFMPVVPILGVLACLGLMYFLPWMTWIRFFVWTI
                     IGVLVYATYGMRHSKLATRPSTEQSETPRPA"
     gene            109370..109564
                     /locus_tag="PK224_02105"
     CDS             109370..109564
                     /locus_tag="PK224_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14560.1"
                     /translation="MLRLEQPGRLSEVLLQPGPQAFGQLPQVEEMLAPESRYLLPQEQ
                     AEQPQAWEEQGASAEQAAPV"
     gene            complement(109903..110427)
                     /locus_tag="PK224_02110"
     CDS             complement(109903..110427)
                     /locus_tag="PK224_02110"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HRB14561.1"
                     /translation="MTPPVTPPGPLEKSRRATMQWLLGFMDHLDRLGYVAAGFSLLAL
                     GMLIFVHAWYAFFLRSNDVPLLPAGLKLLNDLLLVIILLELFRTVVRFLQTEVLELEP
                     YLAVGIIACTRRVLTASAELSHQLEVVTREARQELFQQYVMDVGLNVTVIIVLILGVY
                     LLRMRPTRERPVTT"
     gene            complement(110424..110810)
                     /locus_tag="PK224_02115"
     CDS             complement(110424..110810)
                     /locus_tag="PK224_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14562.1"
                     /translation="MAIKKGDILDERKRFPDSCGLVVKVAGGGEGFQKILCCGHGLTE
                     EDVVPDIISSRGRKKGALLPGAMLEEKRQFPDSCGLRLMVIDGGAGFQGIDCCGHTIT
                     AKAVNGLKFGQPLEDSPMPTGQAGTA"
     gene            complement(111033..112184)
                     /gene="nagZ"
                     /locus_tag="PK224_02120"
     CDS             complement(111033..112184)
                     /gene="nagZ"
                     /locus_tag="PK224_02120"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRB14563.1"
                     /translation="MTLREQIGQLFMIGFTGTTVTKDLASFLTASKPGGVIFFRRNLE
                     SVQQIVDLTNGLQKLSPAQPLLIAIDQEGGRVSRLPSEFTIFPPCGQLGQCNSSELAY
                     SAAATIAKELRAVGINMNMAPVLDVNSNPDNPVIGDRAFGAAPELVGEMGSATIGGLQ
                     DNMVVACGKHFPGHGDTATDSHKELPVVDAGLQRLRDTEFPPFQQAIRYGVASLMTAH
                     VLYRALDPEAPATLSSAVIQRLLREEFRYEGVVFTDDLEMHAIIDHDGIGEAAVRSFV
                     AGCDVLLICKDQDRVMTAMQAMERAVKDGRVTQERLDQSLARVAKLKARYLHPYKPVT
                     ISDARLVVGCRSHKVLLDSWHKAYARIPKPTLVERGQAAPAHDSPVTHV"
     gene            complement(112256..113776)
                     /locus_tag="PK224_02125"
     CDS             complement(112256..113776)
                     /locus_tag="PK224_02125"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRB14564.1"
                     /translation="MKKIQVHAQVGRAENEAAEVLVLLHCEGGSDLTQEAAAIDAQLG
                     GQLAALIQRGEFEGKLGEGLLVHTQGKARAKRLLLAGLGKEKDLRVDAFRQALGIAIK
                     RVRQAKVTSFAVVLPAAILEEIPVQDVAQAMAEGAILGNYQFTAYRSPNGSKPIDVER
                     LTIYTSQASLLPQMTEGIRRGVATAEATVLVRDLCNHPANVMTPTRIVREAKAVAKES
                     GVRLKVLEQKDMEQLGMGALLGVARGSHEPPKFIILEYKGAKAKKGDPPVVLVGKTIT
                     FDTGGISLKPAENMEHMKADMTGGAEVLATMRAAARLKLPLHLVSILPVAENMPGGRA
                     MRPGDIVTTLSGKTVEVQNTDAEGRLILSDALAYATRYKPAVLIDIATLTGACVVALG
                     QFAIGMFGNNDQLKEHVRNAGQRAGERVWEMPLWEEYFEQLRSDVADMRNIGGRGGGM
                     ITAALFLSKFVGDCPWIHLDIASTDWSERERAYLPKGPTGIGTRLLIQFLLDRTLP"
     gene            complement(113858..114496)
                     /gene="queE"
                     /locus_tag="PK224_02130"
     CDS             complement(113858..114496)
                     /gene="queE"
                     /locus_tag="PK224_02130"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRB14565.1"
                     /translation="MVKITEIFHSIQGESTYAGRPCVFVRLTGCPLRCTWCDTAYAFY
                     GGRDMTIDDIVNQVRAFDCDLVEVTGGEPLSQTESLPLLSRLCDEGFEVLVETSGAID
                     TAGVDRRVHVILDVKCPGSGMTDRMYWPNLDRLAPHDEVKFVIKDRSDYEWARDVLHR
                     YDLPARCTVLMSPVFGEVEVRHLAEWILADKLPVRFQLQMHKYIWAPDMRGV"
     gene            complement(114548..115243)
                     /locus_tag="PK224_02135"
     CDS             complement(114548..115243)
                     /locus_tag="PK224_02135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRB14566.1"
                     /translation="MTPLSLSHISGTSRQTDIAALFDVDNTLLPGQASEVRFFRFLWK
                     RGLVGWRELRDSVGWVLRNAPPVSLHPLRERKLYLAGKAAAEVESLAEEFCRAELFPR
                     LSAQGLARMDEHRRAGHHIVLVTGSLDFLIAPLATLLEVPTLLAAKLERQQRRFTGQV
                     CVPLPYGPGKRALIARLTQESRIDLTQSFAYGDSPGDVELLEMVGHPLVVNPIRGMDR
                     IARRNGWPVTRWK"
     gene            115468..115551
                     /locus_tag="PK224_02140"
     tRNA            115468..115551
                     /locus_tag="PK224_02140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:115501..115503,aa:Leu,seq:gag)
     gene            115991..117589
                     /locus_tag="PK224_02145"
     CDS             115991..117589
                     /locus_tag="PK224_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB14567.1"
                     /translation="MTAQDVTTALPPPGDFSTVEALVRTLSGQASIGIFLSDPEGRTL
                     YLNERLRRIAGLPVVPTPGECWRNALAPEDHDPICSEWSGATNAERSFSREFRFRRPD
                     GSLRWVMAEAFPLRSGGGPSGGYVGIVRDITPRQLALDALHASEERYRTLAQQSPHAI
                     LVHADSAILFINEAGARLFGLATAHPIEGRPLWDFFPKEFLHDLPLAEAAPSPSVERQ
                     LVRPDGTVIDVELTAAPVMFDGHPAVQILGTDITERKVIAGQLHRAKKMATVATLAGG
                     IAHEFNNCLTAIMGFSDLALPSLVPDSRVHGHIQQVVLASKRARDLVTQMLMVGRQAD
                     GAKQPLSLDILLKETLRVLKMKLPESISLREWIPGATNPVLADPTQIHELCLNLLARS
                     QQAMTISGGVLEVRLDNMGLDASMTGHDLPLPPGQYVRLMISDTGDGIPADIRTPNMY
                     PLFAPPSTGSKAGAGLAELQRIVSEHGGTLRATSTAGQGTTIEVYLPAMLQPPQTVAQ
                     EPSRERTAPPTEAKEFLAEHDKER"
     gene            117698..118069
                     /locus_tag="PK224_02150"
     CDS             117698..118069
                     /locus_tag="PK224_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB14568.1"
                     /translation="MPSVLIVDDEEAIRRLIRSTLEQAGYHVREAADGKAGLSHYRQS
                     PADLVIMDILMPDQDGLESILTLRREFPEARIMAITGGSDMIGILNFLDVARMLGARR
                     TLQKPFEMQQLLDAVQAEIAG"
     gene            118195..118632
                     /locus_tag="PK224_02155"
     CDS             118195..118632
                     /locus_tag="PK224_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14569.1"
                     /translation="MKTWWTPQLHAVFGGIVVAIGVFVMWEQAATTWALLVATASTAF
                     LWWQGRSIAAVWAWTTLGLGIESMTWPIVTMIRMRMGGSQPTEEQMETILNAVLFGLF
                     TSVFWITFAFGLFKRIREQNAPPAPPPGNGPVPARSKKKRARR"
     gene            complement(118639..121014)
                     /gene="priA"
                     /locus_tag="PK224_02160"
     CDS             complement(118639..121014)
                     /gene="priA"
                     /locus_tag="PK224_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRB14570.1"
                     /translation="MFDHRSSATPPVAPALPPRYADVVLPRRLHRPFTYVIPSSLKGQ
                     VAIGQSVIVPFGSQDLHGLVTAVYDRLPLGAPEQGLKAVRCLAPASPDHLLTSSQIDL
                     SRWVADRYAAPWGQCIKLVAPFVDQVDRRPSRYRPTAQGTDNSSLPEGLGEVETQVLS
                     RLRRRPKGITERTLAQGDKSRVMRAVQILIRRGLVVRCDDAVVPRAARAKKPPSPGAE
                     QAVFARLTVDDLPPPLEAAAWPDTVGRALSQDVYGSFLVQGARATRLWCLVQAARAAI
                     RRGRRVLVVTGDVENAGRLAEALAAAGDRPLLLHSGLSARERAAVWQSAQDSSATILV
                     GTRMAVFAPIDRLGLVWVEGEDDASLKDEQSPRYHAREVARRRAFCDRSLLVLASSHP
                     SLESWSAVQQGEMTACVYRDPSGFPRVQVIDLREYSRETPAGTLLSPPLYEGIQEALR
                     QNALAILYLNRKGFASVLHCGDCGAMPQCDACSVALTFFRRSNHVRCHYCGRTKPVPD
                     HCTRCQSLKLEPVGSGTERIEEAVRRKFPLARVGRVDGETIRRPADARAFSRLLAAGE
                     LDIVIGTQMLFRFGLQARAAFVGVPDADAGLHVPDFRSAERMYHGLMDAVELALPAHA
                     GGAVMIQTRLTDHHAIMAVAAGDDSVFLQQEQAFRQMLQYPPLTSLVRLDVSGTLEPV
                     VAQAAGRWAALLRAEVVSAGNRGTNAGDGSPLLQRSTGFGGETSSIVILGPSPAPHAM
                     ARGRYCWQILVKSLSLEAGKEIVVRTRGVLDRESRRGGLRFDIDVDPVAMA"
     gene            complement(121177..122733)
                     /locus_tag="PK224_02165"
     CDS             complement(121177..122733)
                     /locus_tag="PK224_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB14571.1"
                     /translation="MRTLPMRASLIILSCSLAAVTAIPVHAQNAQSVRLGEAALTYAA
                     GSIRQEMPIDGVINVITGDNQLSGNRMMLGWSGTDTLYLKFFFFFDSAICEIYTVYRR
                     SRKVFHPMTKQYMGYIINRVGVVKVIQIDAALVGVQVVRSYGPLSPGDPVMRFTPPLA
                     EEVVETASGHAEIEAMIVELQADKHMSLVSQGNLVYLDKGQDEGLRSGEYLEVFRTGG
                     GLPERKIGEVKILSTEPHTATAVLSKATARALIGDRVRSKHVSPVEVMQYENGDSDVP
                     AATVQPVMNARTDSAAAMPSESHSMSKPRVERAHGSTRINLDDLADQLEYESGEVKVK
                     PAGVPILEQLAEYLRTAAVSQQVRVEGHSDNMEIGPSLKGVFPTNWELSKARAAEIVR
                     YLIEKGGMDSAKLSAVGYGASRPVASNGTEEGRKKNRRIEVVLDSPEEGTQAQSAKAP
                     LSESDPIPAQFTYNQLDAAPASTAVVPPASATQAGSASVDAPIAPAPSDVAVPVSPVG
                     GETASPPPGS"
     gene            123302..123685
                     /gene="ndhC"
                     /locus_tag="PK224_02170"
     CDS             123302..123685
                     /gene="ndhC"
                     /locus_tag="PK224_02170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRB14572.1"
                     /translation="MAGSELLLEYLSRYFPILMFVFVALAFGAGTLLISYFVQPKYPE
                     PEKLSTYECGSEPFSDARMPFPVRYYIFAMLFVIFDVEVIFLYPWAIVFNKIGLIGLV
                     EMLIFIGLFLVAYVYAWRKGALEWD"
     gene            123676..124203
                     /locus_tag="PK224_02175"
     CDS             123676..124203
                     /locus_tag="PK224_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRB14573.1"
                     /translation="MGLIQLGGHDKDGTPDVITLTVEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQERIMQKR
                     VFLAQPKEVKDALKV"
     gene            124222..124719
                     /locus_tag="PK224_02180"
     CDS             124222..124719
                     /locus_tag="PK224_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRB14574.1"
                     /translation="MSQLAKRIEDTFPGACTQVVEWRGDVSVTVKREALHDIGKFLRD
                     DPAMRFDYIVHVSSVDWPDDEERFEVVYEVYSIRTRQRIRLKTRVPESDCIVDSLTDI
                     WKGADFMEREVYDMMGIRFRNHPDLRRILMPDEYEEGYPLRKDFPLRGKGWRDTFEFL
                     DEPTR"
     gene            124748..125995
                     /gene="nuoD"
                     /locus_tag="PK224_02185"
     CDS             124748..125995
                     /gene="nuoD"
                     /locus_tag="PK224_02185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRB14575.1"
                     /translation="MAQFEDQRTTIYKVDPEHPEDATLPTLRTEELLLNMGPQHPSTH
                     GVLKVILELEGERLVKSTPVMGFLHRGVEKLAEEGTYHQFIPHTDRLDYVCAMYNNFA
                     YCRAVEKLMNITVPDRAEYLRTIVAEIQRIIGHQFWLGTQALDIGAMTVFFYCFRDRE
                     ILLDWFDELCGARLTTSWYRIGGVERDFTPSLFAKLKQFLDYFPPKIDEYVIFLEKNR
                     IWLARTKGVAVISAEDALSFGLSGPTLRGSGVDYDLRKYEPYSAYPKCEFSVPVGKNG
                     DTYDRYWIRVQEIYESVKIIRQCLEQIQDGPIMADVPSVTLPPKERVFTNLESMIQQF
                     KLFSQGFNAPPGEIYCGTEAHKGELGFYIVSTGGGKPYRLKIRAPSFIHMGAFDHMSK
                     GYMIADAVTIFGTYDIVMGECDR"
     gene            126076..128784
                     /locus_tag="PK224_02190"
     CDS             126076..128784
                     /locus_tag="PK224_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRB14576.1"
                     /translation="MGLKPATNPDVEAATIELSIDGRTVSAKDGVSLYDVIASTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKAAAGMVIRTDGDDLFAARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSLVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVAKGDQPLACEFCGDCLAVCPVGAITNKY
                     SKYLYKPWQMKKTTTTCNYCGDGCQMHLETKDTEVVRVTSPLSWKNKWGDRTETAKGH
                     GGLCVRGRFGFQYIDSEQRLRQPLVRTGETLTETPWLDTMQTVIDRLSDIHRKHGPES
                     IAGLITARCTNEELYLFQKLMRAGLRTNQLDSSARYGHLNYVHAVRHAVGVGRPLNDW
                     EDLTKAKAILVIGSNITETNPLTAVRIKEAIRVYHSQVIVVDSANTNIAQLASHPLLV
                     KPGSESFLIDGLVKSVIEQDLIDEASTTRHPQAFSALKQALAQVSLEQVSAQTGIAVE
                     QIREIAAIFAEAPRAIALCAEGIVRQPNGYQNVLKLIDLAWVTGKLGQPGCGVTTVTE
                     EINEQGAVDMGVAPEFLPGQARFDDPGARERFEKAWDVTLPASGSGANFLEILARIRN
                     GQIKALYVIGENPLATLPASMDVKGTLEKLQLLIVQDPFLTETGQMAHVVLPAATYAE
                     KDGTFTNLEGKVLRVRQAVDQVGESLPDWHIMTALANGLGYDWTYESPQDVQNEIMKL
                     LPGYYNLGQPRRVSPSADAYLSNGYEAEVASRYHRQTAQSTSSDDTQPFTLLTGQVLY
                     HSGKMSTHASGLINIEPNNGRIRMNPADVERLGLNDQSTIRLTSRQGSVQAGVKADPD
                     IQPGSCFFPEHFNEPAVKDLMAVEVDAVTGVPYFKSTRVKIEKVGA"
     gene            128844..129416
                     /gene="nuoI"
                     /locus_tag="PK224_02195"
     CDS             128844..129416
                     /gene="nuoI"
                     /locus_tag="PK224_02195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRB14577.1"
                     /translation="MTKKILQAALFYEIWDAMKVTFKHMFHRPITFQYPREQRTIPDG
                     HRGALGLLRYGDGQDRCVGCDLCEAACPSRCIKVISQEDPERPLQRFASEFYIDITKC
                     VFCGYCVEACPVNALAMTKMYEYSTHDKRTLLFDKKRLYEVGERHLEDAKKYLYAHNQ
                     EKNSDESREYRYYFPQSVLKPTQSQPKHLS"
     gene            129427..129951
                     /locus_tag="PK224_02200"
     CDS             129427..129951
                     /locus_tag="PK224_02200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRB14578.1"
                     /translation="MAFVFFAYFAVVSIVAGILTVALKNPVQCGLALLALLLHVSGLF
                     VMLNAEFLWAVQVIVYAGAILVLYLFVLMLLNLKTDDRYFHAKAPYLLAPAAIGLAYL
                     LFLLVRSPFGGSRGDASPAAILLNGDAHAIGIKMFSEYLLQFEIIGVFLTGAIVGAIV
                     LAKTPKSIETRRDA"
     gene            129948..130250
                     /gene="nuoK"
                     /locus_tag="PK224_02205"
     CDS             129948..130250
                     /gene="nuoK"
                     /locus_tag="PK224_02205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRB14579.1"
                     /translation="MIPLSAYVAVSAVLFATGLLGVLIRRNFIIVLMSVEIMLNAATI
                     NLVAFSHYLEAMQGQIVALFIIAIAAGEAAIGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            130252..132255
                     /gene="nuoL"
                     /locus_tag="PK224_02210"
     CDS             130252..132255
                     /gene="nuoL"
                     /locus_tag="PK224_02210"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRB14580.1"
                     /translation="MSDSIDLIVKLIPLFPLMAVIVNGLFGHRYSHDLAHRFAWGSVL
                     MSFLCVLAVFTETLRTGAAREVVVYKWIFGGDLTINLAYLIDPLTCIMLLVITGVGFL
                     IHVYSVGYMHGESGFTRFFVYMNLFMVSMLLLVMGNNYVVLFIGWEGVGLCSYLLIGY
                     YYDKVSAAKAATKAFVVNRIGDAGFLLAIFLVFVNFRTLDYTQVMQNAAQLSPEMATA
                     IALCLLIGAVGKSAQLPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIFDL
                     SPTAMTTVAWIGGLTALFAATIGLVQTDIKRVLAYSTVSQLGYMFLGCGLGAYTAAVF
                     HLMTHAFFKALLFLSAGSVIHALSGEQDIRKMGALKSKIPWTHTLFLIGTIAIAGIPP
                     LAGFWSKDEIMGHAFVHHHYVLYGMAAVGAFLTSFYMFRLTYLTFYGTSRLDHHTAEH
                     VHESPMVMIGPLVVLATLSVIGGFPGVPPENGWFHHFLHSVAGAAGEEHATAPALMLG
                     LMGTATVIALLGWGLAHYFYSGPSSAADALAARMPGVYTTLLNKYFVDELYDRLFVEP
                     TKQIGRLCDWFDRTIIDGLVRSIDRGTDASSAAITWTEKHVVYAGLNIIGYANHRLAR
                     SWRRLQTGMVHQYAAIIVAGLFILVHLILLIWTGSGSNGYLSR"
     gene            132280..133839
                     /locus_tag="PK224_02215"
     CDS             132280..133839
                     /locus_tag="PK224_02215"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRB14581.1"
                     /translation="MLEEFSFGFPILSYLIFLPLAGAAVLWLIEDEDLLKTTTLGITL
                     VELALACLVLVRFVPDSAAMQFAEHARWMPALGIGYHLAVDGISVLFVGLTAFLTVLV
                     VIYSWDTVRNQVRLYFMALLALETTTMGIFASIDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKYVLYTLLGSVFMLVGIALLDINYHQWAVTHHLDHVYSFDLLELLSVPI
                     PLSQQILIFWLMFMGFAFKAPVFPFHTWLPDALVEGPIGMAVMLAGMKLGTYGFLRFT
                     FPLLPDASKSEGVVSIVMVLALAAILYGAVVALIQSDFKRLLAFSSISHLGFVVVGLF
                     ALNFQGLQGSLLTMINLGFSTAGLFFMAGFLSTRQQSSQLSSFGGFAKQVPLLASFLL
                     LIGMASIGLPGTNGFVGEFLILLGAFKAKWWYGAVAVTGVIFGAAYFLWYYERAMLGP
                     LSKNISATIKDLHMREITIALSLSVMILWIGLYPSPFLRMMNGSIQALVDRLDRAKVA
                     SVDTVIHVPAK"
     gene            133844..135499
                     /locus_tag="PK224_02220"
     CDS             133844..135499
                     /locus_tag="PK224_02220"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRB14582.1"
                     /translation="MAEYTLLIILFAPFVGALALIFVSNRQLSLVRGIAAGSAFVSLI
                     ASVYLFYAYDPLKGGYQFIQRIEWSRQLGISLHLGVDGIGTPLVLASGILLFAGIFVS
                     WHIKDRVKEFYIWILILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     LEMTDAEGLKQRDSVGFIFNFAANSKEYAAMKLVLFLSAFAVAALMGILLIYKFSGLN
                     TFDILVLREQAHFSGPLATLIWLLIFFGFASIAPIWPLHSWSPVGHAAAPAATSMLHA
                     GVLMKLGHFSIIRVAFEILPETTRELMPIAAVLCIFSIIYGGLVAYYAKDTKYVIGYS
                     SSSHMGYVFLGMAALDYISLSGAVIYMFAHAMATGMLFAMAGWVYDQTHTRDIPSLGG
                     LSNRMPFISAAFVIGCMASIGMPGTVNFIAEVMIIVGSWNKYPFQVVVAVLGIVLTMA
                     YLFKMMRGLFYGSMAEKYSHSHDAVTVVDRMPLLIMITVSISFGIFPGHLYSVVRSGV
                     DPLIARITKVVPVAEHPMNLPHATSPTVPTSASREALAKVTPR"
     gene            135496..136992
                     /locus_tag="PK224_02225"
     CDS             135496..136992
                     /locus_tag="PK224_02225"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRB14583.1"
                     /translation="MTFSLTLSVSDLLLLLPELFLTAWLCVILIVDFSFKRIVQEQLA
                     YLSILGLVITLACLAWFDVTGITGTLFGKMFVLDRMAIFFKVMILLATILVILLSIDY
                     VHRFAFFRGEYYFLVVMSALGMMFMTSANDLLSLFVTLEFATFGFYVLVSYLRDDMAS
                     NEAGLKFFILGVFAAGLLAYGISLVYGETGKLVFSDMTGAAPTTGLVIGFLLIFAALG
                     FKIGAVPFHSWIPDTYHGAPTPVTAFLSIAPKVAAFAILLRLFLVALATFKPAWALLI
                     VATSILSMTYGNIVAIAQRNVKRLLAYSGIAQVGNVLIGLAAGTKMGTDSILFYLLTY
                     LFANLGAFAVIMAISNAVGSDEIDDYRGLNRRSPFLAFAMLIFLLSLAGVPPLAGFIG
                     KLYIFVAAIKEGLYILITVGLINIVVSMYYYLIVVKKMYINEPLDSSPIKTTGPLRAV
                     IYVGLAGTLVIGIYPQPFLDWAVAATLMFSNLLGPAASIPVPVVPFGG"
     gene            137143..138642
                     /locus_tag="PK224_02230"
     CDS             137143..138642
                     /locus_tag="PK224_02230"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HRB14584.1"
                     /translation="MSMDEPKPQQAVNTDVESVLPTLPWSARILSRLSILGPLVLAFQ
                     KRSIEQRVLAGFGLVFAGILVISAISYRNMTVLIRNGHHDQRSHEFIQSLAATGEAID
                     DAENGHRRFLVTGDESYLSAYNTLQERAPEYLGYLRELTEPGSIQRARVTQLEQLITQ
                     QLREERAAIDLRKETGFESVRTLALAGVAKTALDSVRRLQAQMEQDETKALAQRVIES
                     TTTTRSSIILLIIGALLLFVLLAAVYYLIRHDITARRRIADELQRRGELLEAANKELE
                     AFSYSVSHDLRAPLRHIDGYASLLAKAASISLDDKAKRYLQTISESATRMGQLIDDLL
                     VFSRMGRQEMLRGTVNLNQLIASVLHDLRHDLQDRTISWTIAQLPEVTGDAAMLRQVF
                     MNLVANAIKFTGTQPQATIEIGSRDNGQEEAVLFVRDNGVGFDMRYANKLFGVFQRLH
                     RADEFEGTGIGLANVRRIIHRHGGKTWAEGALGEGATFYVSLPLARTSA"
     gene            138639..139100
                     /locus_tag="PK224_02235"
     CDS             138639..139100
                     /locus_tag="PK224_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB14585.1"
                     /translation="MTLTKPILLAEDNPRDAELALAAMEEHHLADKVILCHDGAEVLD
                     YLYCRGHFKARLQGNPAVVLLDLKMPKVDGLEVLRTIKSDAALKPIPVVMLTSSREER
                     DLVESYALGANAYVVKPVEFHQFLKAVKELGVFWGMINEPPPEGASRVTSR"
     gene            139139..140776
                     /locus_tag="PK224_02240"
     CDS             139139..140776
                     /locus_tag="PK224_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB14586.1"
                     /translation="MTQPLRFIHLESNPADAALIATTLRDAGILCQLKQAQTREEFLA
                     ALRQEGFSLILADTAVPGFDGATALSLARTLHPDVPFLFVSDTQGEEFAIDMMQHGAT
                     DYISKQRLGRLVPSIKRTLRELDERLERRRAEDALRVSEKQFRQAQKMEAVGRLAGGL
                     AHDFNNLLTVIMGHSQVLLGELPSGNPIRAKIEEMQKAGERAASLIRQLMAFSRKQPL
                     EPKILPLNSVVGNVESMLRRLIGEDIQLVIRPDPFNGHVKADPGQLEQVLMNLVVNAR
                     DAMPNGGLLAIETSQTELARTPMHHLHPLPLGQYVKLTVTDTGFGMNADVLAHLFEPF
                     FTTKEEHKGTGLGLSTVFGIVTTCGGGIDVWSQVGHGTTFDLYFPRVNPQASTADATA
                     PQGRLRQGSETILLVEDDSGVRNLVRHELVKTGYQVIEAKNGVEACLTATQQSYHVDL
                     LLTDVVMPGMNGRELAQHLSVIKPNLRVLFMSGYLDDISVNSGMDPHRTTFLQKPFTP
                     DLLLRTVRALLDSSASATGSAPHHSPPDQAPRTARAS"
     gene            140908..141834
                     /locus_tag="PK224_02245"
     CDS             140908..141834
                     /locus_tag="PK224_02245"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRB14587.1"
                     /translation="MRGRCLKDRRSVHRLKLLCGCLLALCLSSGPQAGAALPAGVPWE
                     RLTTGMQVALWSPIEACPQVPSLLMLHIDPERFRFSIYQYRDEGLRAPLSIHDWQQRT
                     DAYVLFNAGLFREDYSYLGVLLKEGRSLGTKKHHSWQGLFAAEPTDGRLRKARVLDLA
                     FDGFTEETPPYREAAQSLMLFDRTGKLRVRDSGKRAFQTVVAEEGEGAILVIKTVDIV
                     SLHHLADCLHRQIPSIQQAMAMDGGASSDVIASPDLLHAAQETTSQATWRSLLAGNIG
                     VHIPLPTVIGISPRTHPRTMPAPDASTSSHSR"
     gene            complement(141846..142271)
                     /locus_tag="PK224_02250"
     CDS             complement(141846..142271)
                     /locus_tag="PK224_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB14588.1"
                     /translation="MRYLTGLLCVGVLCGLLFEWPQPTFAYHSYTLTVQQLRAGLLKA
                     PSTGAKGFLLVDVRSPEEHASGMIPGTDRNIDFREMKARHREIGAALNDHIVVYCQSG
                     HRSNIAAETLADLGYTHVYNVVGSMNAWTEAGFPVSPGR"
     gene            142331..142642
                     /locus_tag="PK224_02255"
     CDS             142331..142642
                     /locus_tag="PK224_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14589.1"
                     /translation="MKSWSRVVAVSLLLGTSGCGEGAIMVKEVDNGGVVVYPYRAEQG
                     NLLSSFRKEAVRLMEQKCPGGWMTVREGETKGRLRQASPIAGANDIVEERRWGIQFQC
                     K"
     gene            complement(142644..143219)
                     /gene="pyrE"
                     /locus_tag="PK224_02260"
     CDS             complement(142644..143219)
                     /gene="pyrE"
                     /locus_tag="PK224_02260"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRB14590.1"
                     /translation="MLQDDLIAAFHKTQSFKWDPKGGFKLASGLTSPFYVDCRTLMAF
                     PHARHLMAQRAWEVTKGLDIDCLGGLEIGAISIATTISDFAYLATPRREWRTFFVRKQ
                     PKDHGLGRLVEGVVQAGDRALIVDDVLTSGGSVLKAIAAAREAGLKVTDAVVIVDRKE
                     QDGRARVEATGVKVVSLLTIDDLMQGRNISS"
     gene            143646..145241
                     /locus_tag="PK224_02265"
     CDS             143646..145241
                     /locus_tag="PK224_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HRB14591.1"
                     /translation="MRHTMCRLLSRLGRDFLIVTCLWTISATHAAEQPAAPAAVLVKG
                     FSFSGNTVISQAELEALTQPYVEQALTLPDLERIAAAVADLYKQRGYTLASAYIPQQR
                     IRFEMVEISVLEGRLGDISVSGNRFYSTEFIRSGFAGVMEARVIRNIDLERALLLLND
                     YPDLKVSAALEPGEFTGSTNVQASVEDKRPIHATLDYNNYGFNNISRNRFGAGIEVGN
                     ALREGAILTFNGIVGDHPERLQFITGGYALPVGVHGTKVVLSGSTGRFDVGAELAALQ
                     IRGKIHTYDLSITHPVVKTRWQSLVLDTGFAAKDNRLFINGALTGHDTVRMAKLGMNY
                     DRVDLYGRTYLSAYGFHGLGEILGGMDDHAAISTRPGADNRFLKGTLAVGRIQSLGHD
                     ALLILKGTGQITSGPMVVIEQMLLGGPDSVRGYQLGERFVDEGYAVTAETRIPLLPSM
                     LPSTQAALFFDHGAGHLRNPQPGERRYSSLSGAGVGLQTELPYYSIHLRADLGFPVGP
                     KPSGGTIAGDRSPTMYLQAATRF"
BASE COUNT        31323 a        45490 c        39642 g        28958 t
ORIGIN      
        1 tgatggccct attccatttt ttcagctcgc tgaggtaacg ccaaaaataa gaaacagagg
       61 aatcatgaat ggaaatggag aactcagcgg caagagactg aagagatttt tcgaactgta
      121 gctcgtcgtg ttccacgtgg aacacttacg cgaaaagaac caacagggtc aagcaagttc
      181 ttccttatca tgcgcacagt gttagcgaga atccattccc ggcaatccag cacaccattt
      241 catgccgatg gttgccgacg atgtctctcg attgcaacca acagaagtga aatagcggca
      301 ggcgtcacac cggaaatacg cgaagcctgc cctatcgatg ccggcttcac acgcttgagc
      361 ttctccctga cttcacctga gaaaccggct attccgtcat aatcaaactg atctggaatc
      421 gctctgtgct ctagcttctc cgatcgctga atctgctgca gttgccgctt aatatatcct
      481 tcgtatttca cttcgagttg aatcgcttcc gcgatatcaa gatcgtcaat attagcgcca
      541 ccgaacactg cgacgatctt ttcatacgtc atttcctgac gcctgagaat ctcagccaac
      601 gattgcgttg gtgccacagt gcctatcttc gcctcttcca tgagccgctt cacatcagac
      661 gttattttcg gcctggtttc cttgaggcgc tgaacttccc gttcaatagc agaccgcttc
      721 gcctccagct tcccgtgcac ctcatcagat accaatccga tgcggtgccc gagatccatc
      781 aggcgaagat cggcgttgtc atggcggagc aacagacggt attccgcgcg cgaggtgaac
      841 atccgatagg gttctctggc atctttcgta attaaatcat caattaacac gccaatatag
      901 gcctgcgacc gatccagcac gagcggctct tcaccacgcc acctcaaggc cgtgtttatt
      961 ccagccataa ttccctgggc accggcctct tcatacccgg acgttccatt gatttggccg
     1021 gcgtggtaga gcccggcaac taatttcgtc tctagcgaat tgtgaagttg gcggggagga
     1081 aaataatcgt actcgaccgc atacccaggc ttcagcattc tcgcctgctc aagtccggga
     1141 atagttttca ggatggccgc ttgcacatcg accgggagac tggttgaaat tccattcgga
     1201 taaaactcgt tcgtatccaa cccttccggc tcgatgaaga tctgatgccg gtctttgtcg
     1261 gcaaacctca cgaccttatc ctcgatcgat gggcaatatc ggggaccgac agagccaatc
     1321 accccgctga acaacggcga tctatcaata tttttcgcaa taatatcatg cgtttgtgag
     1381 ttcgtgtacg tcaaatggca gggcacctgt ggcaaggaca cacgcagcgt tcggtaagaa
     1441 aacggcggtg gaggatcatc tcctggctgc agctccatca cagaaaaatc gatagaatct
     1501 cgatcgaggc ggggtggagt tcccgtcttc aaccgaccaa cctcaaatcc aaaatcacgc
     1561 atgcaatcgg agaggtgctc cgcggacgcc tccccagcac gaccagcggg aaaatgattg
     1621 agaccgatat ggatcaaccc cttcaaaaat gtgcccgacg taagcactac tgcccgcgca
     1681 tgaatagacg taccggcatc cgtgacgata ccggttacag ccccaccccg ggtgagaatg
     1741 cgatcaactg tcccgcccct gatctcaagt gccggctgcg cacggagcgt gcgctgcatg
     1801 acttcgcgat agaccttctt atcgcattgc gcgcgcaagg cacggacggc cggacccttg
     1861 ctggtgttga tcatccgaaa ctggatgccg gcctggtcgg tatttcgccc catttctccg
     1921 cctaacgcat cgatctcctt gacgagatgt cccttggcaa tgccgccgat cgcagggttg
     1981 caggacatct gagcgatccg gtccggatcc atcgtgagca ggatcgtgcg cacccccatg
     2041 cgggctgcgg ccagagccgc ttcacacccg gcatggcctc ccccgacgac gatgacgtca
     2101 cactgttcac tcacgacaag agtcctttca tttcccgatg caaaactcag cgaaaatgcg
     2161 ctccagaatt tcgtcggtcg tgatagcccc ggtaatttca cccagggcgt cggcagcgat
     2221 acgaagatcc atcgcaacta attctcccgc catccctgcc tcgaccgact gtctggcctg
     2281 ctccaccccc tgcaacgcgc gctcgaaagc agcggcatgc cgaagattgg tcacaaggac
     2341 cccttcccgg gactctaacc ccctcggcat caatcggctg ctgatcgcat ctcgcaatcc
     2401 atccagtcca atctgcattt tcgcagagat atcgagcaca ccggcgttca cgggacagac
     2461 gcggataagg tcttctttcg agatccgaga tggcagatcg cacttattca cgaccaacaa
     2521 cgcctgcgcg gcctcccgac gacgaagcaa cagccgatcg ctctccagca acggttgcga
     2581 gccgtccaga agaatcagcg ccaaatccgc gtcttcccat gcgaggtggc tccgacgaat
     2641 gccttcggct tcgacggggt catccgttgc gcggatgccg gccgtgtcga agagtcggac
     2701 cggaatgcca tggatactga cgacctcttc caacatatcc cgcgtcgtac cgggaatcgg
     2761 cgtgacgatc gcgcggtcgc tccgtaacag ggcattcatc aaactcgact tgccgacgtt
     2821 tggacgtccg agaatcgcca ctgcggcgcc ttctctccag atccgtcctt cctgaccgga
     2881 ctgaaccaga ccccgcagct tgctcaaggt ctcatcaagg agccgcagaa gttcgtcctg
     2941 ccgaacgaat gcaatatcct cttccgcaaa atccagcgcg gcctcgatat gcgccaaggc
     3001 cacgactaaa gccgaccgcg tctcctccac ttcacgggac aactcgccgc gccgttgcga
     3061 ttgtgcaatc gccaggctac gcgcagtctt cgcgcgaatc gtatcgagca cggcctcggc
     3121 ctgcgccaga tccagtcggc cattaaggaa cgcgcgcttg gtaaactccc ccggttcagc
     3181 cagtctggcc cctgcggata tcaagcccag gcacagctga tccagcacga ccggcccccc
     3241 atggcactgc acctcgacga catcttcgcc ggtatacgaa tgcggcccct tcatcacgac
     3301 tacgagcgct tcatcgagca ccgcatgcgg acgtgattcc gtcccgcgag gaacggtttg
     3361 gagcgcgccg ggcgagccta cgtccgccag cgccatgaca tgagtttgaa ggtcgtgaag
     3421 ggacttgcct gaccggaggc gcacgacttg agaagcgaca tcaacggctt gaagcccgct
     3481 gatgcggacg accccgattc ccccttcgcc gggaggggtg gcgatagcac agatggtatc
     3541 gtccagcgcg ccatgcatga cgcacccaat cgtgtggaat tactcggatg gactgttgcg
     3601 ccgcttgccg ctgctcttct gctctttcgg ttcgtcgccg gtcacagcat cggcgtccgc
     3661 agcagccact tgccacttct tcccgaacag ccgctcggtc accacctgct gggtgatcgt
     3721 aagcgtattg ttcgtcagcc aatagagcac caatccggcg gggaagttca cgaacaggaa
     3781 cgtcatgaac accggcagaa acagcatgat cttcgcctgc gtcggatcca tcgtggtcgg
     3841 agtgatcttt tgctgaatga ccatggtggc gcccatgacg atcggcagca cgtagtaagg
     3901 atcctgcaca gacaggtctt tgatccagag catgaacggg gcctgccgaa gatcgatcgt
     3961 catatacaga atattgaaca gcgccacgaa gaccggcatc tgcagcacca tcggcagaca
     4021 gccgccgacc ggattcaccc gatgatcttt atagagcttg atcagttcct tgttcagccg
     4081 ctcacgatcg tccttgaact tcgtctgcag ggccagcact ttcggctgaa tgacctgcat
     4141 ctgcttcatg gacttgtagc tcttgtactg cagcggcacg aacatcagct tgatcagcat
     4201 ggtcaagaga atgatcgtga tcccgtagtt gtgcgtaaat tcgtagagga atcgcagcac
     4261 atagaaaatc ggcttggcca ccgccttgac caacccccaa ctcccaaata caaaccagcc
     4321 gaaatcgatc gtatcttcca gcccgacgtt taagcgtttc agcgtgtcgt actctttggg
     4381 cccggcgtag agctgcatcg ccatcgttgc ctcgctctcg ggcgtggcga agcggacacc
     4441 ggccgacacc agcttgtccc cttccttctt cgcgagggca gccgcagcct tctgcggcat
     4501 caacacactt aagaaatact tatcctgaat ggccgaccac ttcacgtcgc ctttgcgttc
     4561 agcctcgccg tccggcgttt ccttcaatac cttgtcgtcg accaatgacg ccgaccccat
     4621 gagcccgatg aaaccttccc cccactccac aatgccgaaa ttggtgccga gagtgacatc
     4681 caccggagtc gtcaaccctt gagtacgaat cgcaatgtcc accacgtagg acccatggtg
     4741 aaaggtcagc tccttctcaa cgtcaacatt cttctgggta tcgtggtacc ggaaggtgag
     4801 gtgaccgaca ggatgcgcgc tatcgagatg cgagagatcc tgggtgacct gatagagggc
     4861 agatccaagg tcgctggtca cggcttgatc cccggtcacc aggctcagag gtcccttgaa
     4921 ccgtcctccg ctatagacca actgcacagg cgtcgagctc tgctcgacag ccgcgctgta
     4981 tcgcttcaat tcccaggatt tgagcacggc gccgcgattg ctgaatttgg cacgaaacag
     5041 ttccgtgtcc acttcaaccg tttgctcctc acttgctgcc ccggtcgcat tgccgggaga
     5101 agcgggcgca ccattcacgg aggagaccgg atcagcactt gcagccttcg ctgaatctcc
     5161 cgcacccccc gcaggcttgc ctgtctcggc cgcagggctt gggttcgtag ccacctgcac
     5221 cggctcagac ggcggcaaca gccccatgcc cttgagcaga tagtcgtaac cgataatcac
     5281 ggccagggag acgatgagga agacgatgac gcgtttttcc atggaattta tgtactgaac
     5341 agaagctgaa gagttattgc cacgacggtt accgtaccgg gtccactccg ccgggatgga
     5401 agggatgaca tttcagcaag cgcctacccg ccatgagaag ccccttgaga acgccatacc
     5461 gctcgatggc atcctgggca tacacggaac acgtgggatg gaaccggcac gcaggcccga
     5521 gcaatgggga cagacaggct cgatagatca tcaagaggac aatgcacagc cgcctcagag
     5581 cgagggttcc ccaaaacggg ccaaccgctg cctttgtaat gtgcctcgcc ataggatctg
     5641 cagctccgca aacggcagat tcaggcactc tcgtttcgga aacacgagaa atgcgtgacc
     5701 tgggactaat gccgggcgca ccgcgcggcc caattcccga aacagacgct ttgcccgatt
     5761 ccgacgcact gctgtcccga accgtcgccc gatcacaatc cccaacatcg catcctggga
     5821 cagacccgga caaatctgaa gattgaatag tcctgttgaa acccgtcggc cgttcttctt
     5881 gatgtgctgg atatcccggc tacgcttcaa gaaaaacgga acagcccttt gccctgccga
     5941 cataacacac ctgcctgtgg gtcaccagtc cagaccaggc ctcaaagacc agaggccgcg
     6001 tgccaagact gtgcgtcgcc gcatttttac tacgaccagg ctaagacgac aggacccacc
     6061 ccaggtgtac catccaccac ctggatgttg cctacttgca gattgcctag cggcaggaaa
     6121 gaatcagacg gtgagacgag cgcggccttt ggctctacga cgagcgataa cttttcggcc
     6181 gttcttggtg ctcatgcgct tccgaaatcc atgttcccgc ttgcgcttga gattcgaagg
     6241 ttgtttaaaa gtgaacgaca ttgcgcactc cttgtcgaga gatcattctt caatgaagag
     6301 gccgcattat aggggcggga caggagggtg tcaatcaacc tgcttgcttc aatcagccct
     6361 gcaagggcgc acggaaggag aaccagagct ggaatgggga aaggtttctg accacgcgct
     6421 cgctactcaa cctcgaactc ttgaggcact cttgcccgac tgtgacaaac atgccacaga
     6481 aacaggcaga gggcgcgtct tgccgcatcg atctaactaa ttgttctgga aagataatcg
     6541 ttccagtccg caacaatctc caccctggca tccagcttgc gttaattaga gcccggggtc
     6601 gtggaccaca acggattgga ggtattccta gcggcctgcg gatgccccag cagaaaccgg
     6661 atgtacgcat tctgaaatgg ccatgcttgt ccgtacgtgt cagtgacaaa aacgagaaga
     6721 gctcgcgaga aacgctccct gcgagataga atgaaacgat gagacgcctg acccacattt
     6781 tccgcacctt ctgcagtcta gcccttgccg gactcctggc aatgagcctg ggcgggtgtg
     6841 tcgaaacggt ccccgacgag ctggtcgaag ccattgaggg catcgaccgc gacctcgtca
     6901 cgctgcgggc atccgacatc agccccgaag cctattcaaa attctcccgc gaatggatct
     6961 ctttacgagc ccgcattcaa tccgaggaaa atatcgtccg ttggccgtgg gaagaaaatg
     7021 agctggagac cgacctggaa agcctgcaag tcgaagggtc gcagctcgtg gccgacgtga
     7081 acacgcgcct ccaagcacaa cgcaccgccg ccgaaacaaa actcgccaag gtcgagcaac
     7141 ggctccgcct cttgaacacc cgcgtaggag atatcggcag ccgtgtggta ctcggcgaac
     7201 atccggttga aaccgaactg cttgtacgac aagctcgcct gttcattgaa cagggacagt
     7261 tcgatcatgc catccaggct tcagatcgtg cgggaaaaat actgctcacc caaaccgcct
     7321 tgctgacaaa cgagttgggc cggtatgccg atgacgacct cattgcagcc tggcgagaat
     7381 cagcccaacg aaccattgaa tggtcccgca cccatcgtgc gcaagccatt gtcatcagca
     7441 aggccgaccg agtgctgtct ctgtacaaaa acggcaagaa agtattgacc tatccgatcc
     7501 gcctggggtc gagagggatc cgggcaaaac agcactacgg cgacggcgcg acgcccgaag
     7561 gcgaatatcg cattcaccgc aaacgcgggt cgggacagac cccgtatttc cgcgccctga
     7621 ttctcgacta tcccaatgcc gacgatcgac gccgctttga agaagctcaa caatcagggt
     7681 tgattcccaa gagccagcat atgcccggcc tgatccagct ccatggcatc gcacaaggta
     7741 tcacggatca accgtacggc agcatcctgt tggataatcc tcagattgct gtgctctttg
     7801 accaagtttc tgttggaaca cctgtcacca ttgtcggcgc actggaatcg cagaattcca
     7861 tttctctcgt actggccgat ttaggtgatc aggaagaaga aacctagatc cccatccttc
     7921 cgcgcgactg ctccaaccgc acagcaccca cacccactca ttcaagaaac tcctgcgcag
     7981 tctctggctt ctctttgttt gcctttttac acgctcgaac ctaagatacg aaagagccga
     8041 ggtccatcga ttcgttcctc cactaacgga agagagcgtg ttatgccgac ccgtcccact
     8101 gcatcgctcc tgctcgacaa cccaaccatc agcaaaacgc tggacgacct ggcgagcagc
     8161 catacgatcg agcgtatctg gacacgagac catactctct ggaagcccag tccgactgaa
     8221 atcgataatc ggctgggctg gctgacggtg ctcgaccata tgcaggacgg gctcgccgag
     8281 ttacggagct tcgagcaggc tgcccgggaa gctcgcatca ccgacgtcgt gcttctcgga
     8341 atgggtggaa gcagcttggg gcccgaagtc ctccgctgca catttggttc ggccaaaggg
     8401 tcccccaaac tctgggtact ggactccaca gtcccaggct gggtccgtca ggtgacgaaa
     8461 gcgattcagc cggcgcgaac cctctttctc ctggccagca agtccggcgg aacgattgaa
     8521 gtgatgtcac tcttcgctca cttctgggaa cttgtccggc ggaccaaagg caatcgcggc
     8581 ggagcccagt ttgtcgccat cacggatccc ggcaccggac ttgaagagct tgcgcgcgag
     8641 cgcgggtttt ggcgtacgtt caccaatcaa ccggacatcg gcggacgata ctccgtcctc
     8701 tcctactttg gccttgtgcc cgccgcactg ctgggactcg acgtgagcaa actcctgagc
     8761 cgggcgctcg ccatgcgtga ggcctgccga cacaccactc acccaaaaga caacccggga
     8821 gcagccctgg gagggatgat ggccgcgatg gccatggcag ggcgggacaa ggtcactctg
     8881 ctgacctctc ccgcactcaa ttcatttgga ctctgggtgg aacaactcct cgcggaaagc
     8941 accgggaagg aaggaaccgg gatcgtcccc gtggcaggtg agcccctggc ctccccgacc
     9001 gcctacggag ccgatcgact cttcgtcacg cttcgactca aaggcgaccg caatgccgcg
     9061 ctggatcgat ccatcatcac gttgcaacgg gccgggcatc cggtatttcg attgaacctg
     9121 gcggatcgct acgacctcgg cggggaattc ttccgttggg agttcgccac cgcggtcgca
     9181 ggacacgcgc tcggcatcca tcccttcgat cagccgaacg tgcaggagag caaagacaac
     9241 acaggacgcg tgctaaaaga agttgaaaca cagggacgat tcccctcact gccgatgctc
     9301 acgcccaggc aggcactgac tcaattgctc gaacaagcga cccccaatcg ctacctggcc
     9361 attctggcgt acaccactcc cagccctcag ctggacgcct caatacgtgc gttgcgcaag
     9421 gcggtgatgc tgcgccacca catcgccacc acggcgggct atggtccccg gtacctgcat
     9481 tccacaggcc aactccacaa aggtggcccg aacagcggcc tgttcctcga actcgtggac
     9541 agcatgaaac ccgaccttcc ggtccccgga aagttctatt ccttcggcac gctggcacag
     9601 gcgcaagccg tcggcgacct tcaatcgttg cagacccatc aacgaccggc cgtccgcatc
     9661 gccctcggac ccaatcccgt gcgcaccatc cgaagcctca ttgccatgct cacaccggcc
     9721 agagccggtc gtcgtaaacc cgcggcaaaa aaacgtcctc tgcccaaacg ccgcactcac
     9781 cgttgacatg tcccaggctc cagcaatcca caccttcacc gatgcccagg aactgacccg
     9841 tgccgctgca ggtctcttcc tggaagtggg caagcaggcg atcgccgagc ggaatcgttt
     9901 cctcgtggct ctctcgggcg gctcaactcc gaaagcactg tattcgatcc tggccaacga
     9961 caaatacgca cagcaactcg actggagcaa ggtgcacttt ctgttcggag acgagcgcag
    10021 cgtaccgcct acgcatgccg acagcaattt cgccatggcg aacgccatat tgttcagccc
    10081 gttgcacatt ccctcagcgc agattcacag gatgcgcggc gaagacccgc ctgaaaccgc
    10141 ggcagctcaa tacgaaacca ccctgcgtca cctcaccaca gccgtccccg ggcaatggcc
    10201 gcggttggac cttgtccttc tgggcatggg cgacgatggc cacaccgcct cactcttccc
    10261 cgacacggca tcactgaccg aacagactcg ttgggtagtt cccagcacgt ctccccaagg
    10321 aacgcgggca cgggtgactc tcaccctagg tgtgatcaat cacgcgagtg tggtactgtt
    10381 cctcgtcgcc ggaaggaaca aagccgcggt tgtgcgacgc gtactggagc agcggcccgg
    10441 tgatcccggc ccgtatccgg cagctttgat tcggcctgag accggacgac tgttgtggta
    10501 tcttgaccgc gccgcggcat ccgaattgac cgcaaccact gacgattagt catcgcaaga
    10561 ggtaccatga tcctggcagg cgatatcgga ggcacaaaaa ccaatctggc gctctacgac
    10621 tggacgactg aacgggtcga gccggtgcgg gaggatagct ttcatagcgc ggactataag
    10681 accctcgaag aaatcatcga agaatttctc agtgcaccgt tgcccaagcc acaagcagaa
    10741 gacgagctgg gagacgagcc catcgaagcg tcggcagaag gaactacgga ggcaccgaag
    10801 cttcccccag agcccatcaa attaacgacg gcgtgcttcg gcgtggcagg gccggtgatc
    10861 gataaccgtt gccgcaccac caatcttccc tggttcattg atggggcgac actcgcggag
    10921 cgattcgcca tcccccaggt gcgcctgctc aacgaccttg aagcaacggc ccatgggctc
    10981 ctcctcttga atcccgatga aatcgtagta ctgaacgccg gcgccccgcc gaagaaaaaa
    11041 caggccctcg ccttgatcgc agccggtacc gggctcgggg aatgcattct ctattgggat
    11101 ggaagtcgct accgccccat gccgtcggaa ggcgggcaca cggactttgc ccccaacagc
    11161 gacagcgaaa tcgacttgct tcgccatctg cgcggcagct acctgcatgt cagttacgaa
    11221 cgcatcgtat ccgggccggg cctccatgcc atttatgaat atctccgcga caccaaaaaa
    11281 aatgagccga cctggttggc ggagaagatc aaagccggta atcctgccgc cgagattgcc
    11341 gaggccggac tcaagggcca ggccgaaatc gccaaacaag ccctggattt atttgcgtcc
    11401 atctatggcg cggaagccgg caacctggcg ctcaaggcgc tcacactcga cggggtctat
    11461 gtcgccggcg ggattgcgcc caaattactg aagaaactgc aggacggctc gtttatgcgc
    11521 gggttcacaa ataagggccg ttacaaacgg atcatgagcc agattccggt gaaggtcgtg
    11581 atgaacgaca aaaccgcctt gctcggcgcc gcctcgatgg cagcacaact cacatcatcg
    11641 accacatcat gacgccatcc atcatgacac tacaatttga tcttgattcc gaaaagaaac
    11701 aggcggcgct gaaagccact gaatatgtgc aagacggcat ggtcgtcgga ttggggacag
    11761 gtacgacatc gaagcatttg atcatcgccc tcggcgagcg cgtccgctca gggctcaaaa
    11821 tccaagccgt gccgacctcg cacgacacag cggctctggc caggcaatcc ggcattcccc
    11881 tcatcgagtc cgacaacgca tggatgatcg atgtggccat cgacggagcc gaccaggtcg
    11941 atcccgcctt gaacctcgtg aaaggcggcg gaggcgcgtt gctcaaagaa aaaattgtag
    12001 ccgccgcagc gaagcgcttt attgtgatgg tcgaccacac gaaactcgtg ccggcgctcg
    12061 gcggcagttt tccattgccg attgaagtcg tgccgttcgg atggggcagc acggcacgga
    12121 acatcgaaca agcctcagga ggcagagcgg tcttgcgcga gcgaaacggc gtcgtgtttc
    12181 agaccgaagc gggccacgtc atcctcgatc tgcacatgcc caagatcgaa gatccggcca
    12241 ccctcgagat agaacttaac cagattcccg gcatcgttga gaccggactg ttcattggac
    12301 gcaccagcat cctgatcgtc ggccatgcac agggtgccga cgtcaccctt gcagcggagt
    12361 catgacgggc gaccggaccg accctcccgt cactcggcga catgcggcgt tgctggccac
    12421 acgagtcctc accaaagcag tggtgtggtt gggagcctgg catggcgcaa cctcctggtt
    12481 cttcgtgccc gcggcctgga gccgctcgaa tccgccacac aaccatcgac ggccgaagac
    12541 acacactatg ggttcatcta tgacgactca cgatccctac agactcccgc gccatgtcct
    12601 cccgtctcat tacgacttgc gtatcgagcc ggacctccag gcacactcgt tcagcggcca
    12661 cgaggtcgtg acgctgacgg tgaccgaacc gactactgag attctcctga atgcgaccga
    12721 attggaggtc tcgacagcga cattgtccgg tgaagggacg ccgccgcgca ccgggaccgt
    12781 acggatggac gaggagcacc agcgatgcta catcaccttc ccgtcggtca ttcaacccgg
    12841 cgcctggaaa ttgagcctgg cgtttcgcgg cacattgaac gataaattgc gagggttcta
    12901 tcgcagcagc tacaaggatg aacagggcca ttcgcacagt ctggccgcga cccagtttga
    12961 ggcgaccgat gcgcggcggg ccttcccctg ttgggatgaa ccgcaattca aagccgtctt
    13021 cgccgtcacc ctggcaatcg atcccgcact gaccgccatc tcgaataccc ggatcgtgga
    13081 cgatcgacaa gagggcggga agcgagtgct gcgcttcgcg gaatccatga agatgtccac
    13141 gtatctcgtg gctttcatcg tcgggaaact cgacgccaca actcccacca tggcccggca
    13201 gaccccggtc cgcctctggt ccgtgcccgg caaacaacac ctgaccccgt tcggtcagga
    13261 aatcgccgtg tactcgctca attttctcgc cgactactac ggcatcccct atccgggcga
    13321 caaactggac ctcatcgcca tccccgactt cgcttccggg gcgatggaaa atctgggcgc
    13381 gatcacattc cgtgaaacag ccttgctgct ggaccagcgc acggccacac atgcggagca
    13441 gggacgaatc gcggatgtgg tcgcgcacga gaatgcccac atgtggttcg gcgacctcgt
    13501 gaccatggcc tggtggaacg ggctctggct caacgaggcc ttcgccacct tcatggaaat
    13561 gctggtggtg gatgcctgga aaccggagtg ggaacgctgg acggcctttg gcgtctcacg
    13621 cgctgccgct ctgtcggtcg acggcctgct gagcacgagg ccgattgagt tccccgtgcg
    13681 cgcgcccaaa gaagccgaag ccatgttcga cgtgctgacc tatgaaaagg gagcctccgt
    13741 cctgcgcatg ctggaacagc atatcggtcc gacggtattc agagacggcg tccgccacta
    13801 tctgacgacg catgcctatg ggaacgcaga gactaccgac ctgtgggtct cgctcgcgca
    13861 tgcgtcacaa cagaatgttc cggcactcat gaacgaatgg atcttctcgc cgggatatcc
    13921 cttgctctcg cttgccatcg actcatcctc cacgctgacg ctcacacagc gtcgcttcac
    13981 ctacgccgaa ggctctacgg cggcatcttc cggggcaccg gcgcaactct ggcaggttcc
    14041 gattcagttg cgcatccaca ccgcgcgggg cgctgaaacc cggcgtgtga tgctcagcga
    14101 tcaggagaac cgtatcccgc tgccgaagga ttggacatca gtactggcga acgagggcgg
    14161 gcacggattc taccgcgttc gatacagcac agcgctcctc aacggcctgc aacagaccgg
    14221 cctccagacc ctggcccccg tcgaacggtt caatctcctg aacgacacct gggcctcaac
    14281 catcgccggc atggtgtcac ccgccgacta tctcagcttg acggaacact tccgtggcga
    14341 acaggacccg catgtctggg ccgtgatgtt ggggtcgttc tccaccatga atcatctgct
    14401 gagcgaggag gatcgaccgc tgttggctgc ctttgtgcgc aatcgactca ccccgacatt
    14461 ccaggatctg ggatggacac cgcgtaccga tgagcgcgat ctcgtcaaag aactgcgcgg
    14521 tgacgtgatt cgcgccttag gcacattggg tcgcgacccc agcgtccaaa cccaggcgct
    14581 ggaagcatac actgatctgc aacagcagac tcgaccgatc gaccccaatg tgatccccgc
    14641 gctcgtttcc atcctggcct tcacaggcga cgcggctcgc tacgaggaat ttctcaaccg
    14701 cttccacaag gcctccaccc cgcaggaaga gcgtcgctat ctcttttcac tcgcggcctt
    14761 ccggataccc gaactgctgg agcgcaccct ggcgaaaacc ctgaccgacg agatccgcac
    14821 ccaagatgcg cccttcctcg tcagcagcct gttgcacaat gtgtacattc gtgaaaaagc
    14881 ctgggagttt gtgaagacta attgggagcg aatggaccgg cagtttccca agagcggcct
    14941 gcgccgcatg tgcggaggga tcaccggtct ttcgactccg gaattggagc aagacgtcag
    15001 agcgttcttc acatcccgga agatcgacct cggcgggaag actctggagc agtacctgga
    15061 gcagctgcac atcgccgtac gattccggga acgcgaccgc gacgccattc gcgccgtgct
    15121 ggcacgcgtc gcgatatagt gggaacggcc gagtacatcc agccggccgt cactcgtgcc
    15181 gaagcacagt caacggaggc tgcccgagca gtcggtaggt actgaggaac cctacgatga
    15241 gggtgagcaa cacagtgcaa cccagcccga tgcccagcag agacggctcc agcgaccagg
    15301 gcaactccag aatgtagcgc agaatcgccc acgagaagag gctggccagg cccacgccga
    15361 tcacccccgc cacgcacccc agcactgcat attccgctgc aaacgaccgg gcaatcaacg
    15421 cacgggtcgc gccgagagcc ttgagaatca cggcctcata gagacgacga taccgcgtag
    15481 ccgccagagc cgccgccatg acgagtgccc cggccaacag acaaaacagc gccaccgcgc
    15541 ggatagccag ggacagccgg tcgagaaccc gggcgaagct gcttaagacc tcgccgatgt
    15601 taatcgccgt cacattggga aacgcggcga ccacggccga ctgcagcgcc acctcatcct
    15661 gcgaggacac ccgcaccgtc gcgacatagg tcatcggcgc cccgtccaat gctcccggtg
    15721 aaaaaatcat atagaaattc gtggagaaat ttccccactc aacctttctg atactgctga
    15781 cctcagcccg cataatcgtc ccctggatat tgaggtccaa agtggtgccc acatcaatgc
    15841 cgagactctt tgccgcttcc tcttcgacag acacctgcgg tcgcgcaaag acctgccccg
    15901 gcttccacca ggcgcctttg acaatcctat tgtctttagg cagttgctcg agaaaggtca
    15961 gcacatactc acgattcaca taccaattct tccgcttctc ttctttggac tgaccgggct
    16021 ggtcttcctg ttcggactcc cgctcaaccg cgactgcttg gccattgatc gcatgcaatc
    16081 gagagcgcac cagcggagtc agatcaggag ccacatcgcc ggtgcgccga tgaatcaagg
    16141 cggaaaagcc ctccgcctga tccggctgaa tatcgacaaa aaagaaggtc ggtgaatcgt
    16201 tcggtcggtt ctcgccgact tgacgcacca aggcctgttc cagcaacgca atcgcgagaa
    16261 tcaccatcac cccgacaccg atggagacca tcaccccaag ggtttgccct ccgggacgtt
    16321 gcatgttgcc gagcgcctgt cgcagagaaa gcgcacgcgg gctgggcaac acacgtatcc
    16381 cgaggaggag tgctttcgcg gtcacggtta acgccaccac agcgaccagg aggccgccga
    16441 taaagagccc tccgatcatc agagacccgg cctgccacac cgagaggccc gccaaaccaa
    16501 gcccaatgcc ggccgccgtg acggcacgaa caggatccgc cgtgacgacc tgtgccgcgc
    16561 ggctccacac cgatgtctca ggccgaacac cggggcggat ggcaccttcc acttcacgcc
    16621 tgaagatgac cgcaggcttg atggttcgaa tcgtcagaag cggccacaga ctgaacagaa
    16681 gggtggtcag cacacccaag cccaagccct tcgagagcgg cgccagcgct ctccaagaca
    16741 gcacggacgt aaactcgacc tgctgcagga catccgtcgc gagcaaggtg gagaccgcct
    16801 gcggcaaaac ggtctgcaac aggagcccga tcccaatacc gactgcgctg cccagcaccc
    16861 ccaacccaac tgcttgtccc agatacgaat agatgatggt cttcgtatcc gcgcccaatg
    16921 tcttgaggat cgcaatcgat tggagttttt ctcgaacgaa cgcttgaatc gacaaggcca
    16981 cgccaatccc gccgacgaac aacgccgtca agcccactag ccccaagtac cgggcgagct
    17041 gatcgagaaa ctgcttgagc tgtggctgag catcccgata ggaagacagc cgtgcggact
    17101 ccgcggaaag gcgcccctgg agctcatgga gcagcggaga caggggcatg gaagcgggta
    17161 acttgaggag atgccgttcg cgaagacgac ttcctggttt gacgaggtcg gcagccacga
    17221 gaccgtcttg ggaaatcagc actcgcggtc ccaggctgaa catattcgcc attcgatccg
    17281 gctcggtatg aatgacgccg gtaatccgga atgacgcctg tccgattttg atcgcatcgc
    17341 ccacgaccaa ccctaaccga atcagaagac tttcctgaac cactgcccca tggcaagcct
    17401 ctcgacagct gtgtcctgtt tggcgcagca actccatcag cggccggtca ggttcaaccc
    17461 gtacaattcc atacagggga taccctgatt cgatcgcttt caattcgacc agctgcgtca
    17521 catctgccgc gcccacggcg cgatcaattc gtgccaccat tgcgaccagt tcactcactc
    17581 gtgtgcgcaa aatcccgcgc tctgcgagac tctgaagcac gaccgagcct gccgcgccta
    17641 caggtttgga aaggcgaatc tccaggtccc cccccagcaa cccgcgcgct tccttcagaa
    17701 cggcccgctc aacattggta gaaaagagtg agaccccgac cactgccccg actcccaggg
    17761 caatgcagcc gagaaagtac agaaagtgcc gccaggccga acgtagttcc cgccaggcca
    17821 tcatgagcca gaagggaatc atgattcgag atcaggcatc ctatggtgga gtgacgccag
    17881 ctgatccgaa gccacacgcc catcccggag cgtaatcacc cgttccatgg aagcggccag
    17941 gtgctggtcg tgagtgacca ggaccagggt cgtcccggca tcgcggtgca aggccatgat
    18001 caattcgatc acctgctgcc ctgtggagga gtcgagattg ccggtcggct catcggccaa
    18061 cagaatcggc gggcgacagg caaacgcacg cgccaccgcg acccgctgtt gctctcctcc
    18121 cgatagctgg accggatagt gcgacatccg atgtcctaac cctaccgcgt gcagcaattc
    18181 ctccgctcgc ctcggggcct gagagtcgcc cgcgagttca agcggtatcg acacattttc
    18241 cagcgccgtg agcgttggaa tgagatgaaa cgattgaaag atatacccga cattggccaa
    18301 gcgcagtcgc gccatcgcct gttcaccgag cgccgtgatc tcgaccccgt tcaaccaaat
    18361 agttccagat gtcggtcggt cgagccccgc aatcaggcct aagagcgtgg acttaccgct
    18421 cccggaaggc ccgataatcg ctaccgtttg cttttccgga atgtctatcg tgacatcgtc
    18481 aagaatggtc accgtctgac cgcctgcggc caactgcatc gtcacatgct gagtagcaat
    18541 cattgcttgg atgattcctc gatggagtgc ccgcaaaacg atttcatatt atactgtaca
    18601 gatgctgacc tgctcggagc ttttgaagtg tatcgcgcgc ctgggaacgg cttgcctcat
    18661 ggcattggtc ctcagcggac tatcaggatg cgatcaatca aatacgtcca ctgtcccttc
    18721 ctcacccgac agccttgcat ctgcaactcc tccatcctcc gaacaaacac ccccccgcga
    18781 atcgcttcct gccacgccga gtccttcacc tgacgatcgg ccccgtatca tcgcgtttgg
    18841 agacagcttg actgccggac ttggagtctc cccggaacag tcttacccca cccaacttca
    18901 gaaacaactc gataccttag gctatcacta ccaagtgctc aatgcaggag tcagcgggga
    18961 cacgtcagca ggaggactcc gccgtgtgtc ctgggtcctt gccggcaagc cacgggtggt
    19021 gattcttgag ttgggcggga atgacggact gcgcggcctc gggttgcctg aaacccggtc
    19081 acacctcgac gcgatcatcc gacagttcaa ggatgctcac gtacgagtga tcttagcggg
    19141 aatgaagctc cccccaaatt acggcgaaga atacaccgct cgattcgaag ccatatatcg
    19201 ggatctcgcg cagctccatg ggcttccact tatcccattt ctactggagg gagtaggagg
    19261 ggaaaaggcc ctcaatcaag ctgacggcat ccacccgacg ggggaagggt atcgtatcgt
    19321 ggtcgagaac gtgctccgga gcctgcttcc tgtgttgaag gacgcatcca ccaacaactc
    19381 gtcagcaaaa aagaagcagg cgtgaaacat cctccccgcc acatcaagcg ggtgaacatt
    19441 tcacgccatg aagtcctgaa agccggttag ctcttcttga agaaggtgtc gtagaccccg
    19501 aaagccaaga tcagagcgat caaggtataa tacaggcccg tgattccact gtagtccggt
    19561 ggaccttcac tgacattggc aaacgcactg gttgcctgaa gcgccccaat accggcaatc
    19621 aatcctgcta tcagcttgtt catgatcccc ccttgtaatc aaggaatacc caaagagggc
    19681 cgcattgtac tgaagtgacc ctggatgacg caagggggtt tcacaaagag ggaatagggg
    19741 agaaaatcgg ggtggcagct tccgtgacca ccgacttgcc aatcaggcct ttcccaaaga
    19801 cttgagcatc gaagacacat ctcggcaggt atccgaaaga ccttccaagc gacgcccctt
    19861 gtccgtcaaa agagcggcca tcgctttcac ctcgacttca acctgctcaa cctgccgggc
    19921 aagatcggtc gtcttgctta ccgcagcctt ctgcgcctca atctcccgtt gaagatctcg
    19981 gcaaatgacc tgtagtgact tagattcatt aaccgaagcc tccagatcct gggtgagacg
    20041 ggccacgacc gcttctcgat tgcgaatgtc cgcatcaagc tcaacctgag cgacttcgct
    20101 ctctcgcctc cgttcatcca tctgttggat cacctgggtc caggtcaaca caaggcccgg
    20161 cccgatagaa ggttggtctg gaaggggctt cccggctcga atggcctgaa ccgttcgctg
    20221 catccactcc gtaaactggt tcaattcgct caacttcata tcaccggagg acacgacagg
    20281 ggcggccggt ttttctgctt cggggcgggg agcggcaact ctgggcttgg ggacaactcg
    20341 tgcctttgac caccgctgat actcaagcag cgcagccaca ccgtgtcggc agatcggttg
    20401 ctcggataac gtgcaagaac atttggcttc cagatgacca tcaatgagac gaatcgtctg
    20461 ctcgtacaac ccggaattgc ccatgaccga cgacgtcact tccgtatccg acgcatccgt
    20521 catttgcacg cggcgttctg cctgatactg ctttccgatt tggaacgcgt tgggctccac
    20581 caccgagcta atcatggccg cctccaacaa actcagacga tcggccgaac acgcgattct
    20641 gcccttcata gtgtcacctg tggaatgatt agtaccgctg agtcgtcctt gcagcctacc
    20701 gagtgatcgc cgcagtgtca tggaatcacc acctttgacg tccacctatc cagcgagact
    20761 tgagtgtatg acaggttctc ctgaacgacc atcccgcagg cggggggaat tgtgagaaat
    20821 ggatcgaggc cgcgcagaat tttcgtgccg ccactggaga ttcttatcgg cagcgtcgag
    20881 gaggtccttg agccccctgg ggggtcagcc acacatagtc agcaatccct tcttgcatgg
    20941 cctgatgaag ctctgcagcc cgctctcgct cgaatgtggc catataaacc gtgacctgct
    21001 tgagcccttc cggaactcgt cgagccaccc gatccgggac cggcaaccgg tattgcacat
    21061 gacctccgcc cgtataactg agcagggggc cctgaccgga gctggcctcg acacgtagtt
    21121 gcttcatcgc cgctgctaag gtcttcgcca tcccctcgtc tctgaccata gaggcttcat
    21181 acatggtctg gtagtcctcc ggagacccac cgccgtggca gtcctggagt tgagataaaa
    21241 tccgggaccg gtaggcaggc gcatccacga tgacctctcc ctccatgccc cattgacgcc
    21301 actcaggctg ttccttggct tggaccaaac cctgcttcac cacctgccgg atcagactct
    21361 tcggcggatt cattgccaga agcgaaagac gttgatcttt cgcgaattgc gccaatggct
    21421 cgtaatcttc aaacgcgccg ccccaattct gcttccacgc gacttgctcc aggaactccg
    21481 agcgaatact ctcctccgaa cgcagatagc gagcgagcgc cggctgaccg tcccacccga
    21541 acatttccat cgcgagccac ggacgacgcc cctggctcat aagggagcga agcaccgtca
    21601 acgccgcgtc gatatgcgat cgattgtgat gttcttctcc caggtagatc acatcgtacg
    21661 tggccagccc ctccaaccac ggctgcatcg gtactgcgag accggtcctg gcatccacca
    21721 cctggcccac cgcccattcg tcccagaccc gatgagtgac catcgctgag cgatctttcg
    21781 cctggcagcc gaacatcatc aagacaagac caagcagggc gagcaagcgg cccgagactg
    21841 ggcgagtctt ccggaaaagc gatggagtga atgtattcat attgtctacc taacatacgg
    21901 acccagtagc cgcaaggcag ctcccttgac tcccttacga caggcccgat actctgcccg
    21961 gacacgcagc agctttcatc cccgcaccac cgaggaatcc gtacaccgct gatcgaatca
    22021 tgcacgttga ctctgacaac ctggcggcct ttcggaaaca gatcacgtcg gcgcggtcac
    22081 tggatgcaca agcctgggag gcctcgcgtc acctggtgaa tgccgcgcac tggccgatga
    22141 cccttcaagc gctggtcaac gcgatcgaaa cctcgactgt cccggatacg atcaaacgca
    22201 gcctggttga agccctgccg ccggagcacg agagcacgaa ccgcatcgcc tccacggaat
    22261 cgctcaagca cctgacggga ctcccgccct ccaaagcctt acgggcgctt tgcatctttt
    22321 ttggagtgcg ctcgaaacca tcatcgaaat ggccgttacc ggcggtcacg gcagacacca
    22381 tcgacgtatt gatccgccgg caccacaacc ccttcgacct gctgacacaa gagaacccgg
    22441 cttccgtctt ggatctggga gccggtgacc tttcgtttgc cgaagagctg gccaccctgt
    22501 acgaacctca gctagccgcg caaagcagac ccctcatttt ccactgcctt gaccggctgg
    22561 atcccggctc gcaactgggc ggtcccctgc acgctcatcc gcttcgtctg aaccgtctgc
    22621 gttccagacc cggactgcaa tttcgctttt acggcgatca agacatgttt gcactcgccc
    22681 cgctcgaaca ggatcaccgt cttgccgaac gctacctgat cgtcacctgc tgggcacccg
    22741 ccaccccgac ctttgcctat gaacccacgc gcctttcccc ggcctgtctg gccgaagagc
    22801 tgcgacgcac aaaaggcgag tcccgacaag tccggcatgg caaagagtcc gcccttgaag
    22861 tgcaacacgc gggtcggagt ctcctttttc cgccctggaa attcgatatc cgcggcccgc
    22921 ttgccctctt ggaattgatg gccacacgcg gagcgctctg cgtcttggga gctgttgact
    22981 cccaagtatt ttgggaaatc ctgtcgcaat tgatcgaaga tccgcgggtc agaccccgtg
    23041 atctcatcct gtctgctcag aatgtacccg aggtcttcgg ggatacctac cacaagctct
    23101 cagccctccc gatcggcgga tcctgtctct tatccgacct cacaccgttg cgccgaacct
    23161 ttccttctgt acttcaccgg cctgccgacc ggactgcagc ctaccgattc cgacaagtga
    23221 ccattcagcg cggcgctctg ttcgaaggtc gtccagccag cagcacggcc cggcggtttc
    23281 aggggatggc ggaagaaact cccccctggt ttcttactct cgttcctgaa ccagtccaga
    23341 cggcgtgaca ggcgcacaaa agtcgcgcca acgtggcagt ccctacagct tccgcctggc
    23401 ctcccgcgtg aaagtccgta taaattgacg gtctccagac cctttgttag acttctggca
    23461 taacgcccct ggtgccgacc gtaaccctct tcagaaacat cccatcgtga attcttctca
    23521 atctatcaaa tcgttacagg acaatatcgc gcaggtcatt aaagggaaac cacaggtgat
    23581 cgagatggcg gtcgtctgcc tgctggcacg tggccatctc ctgatcgagg acgtccccgg
    23641 tgtcgggaag accacgttgg cccatagcct cgcgcgctcc ctcgattgct ccttcaaacg
    23701 tattcagttc actagcgacc tgctgccctc cgatatcgtc ggcatttcca tgttcaatcg
    23761 ccagaaacaa gcgtttgagt tcatgccggg cccgctgttt gccaacatcg tcctggccga
    23821 cgaaatcaac cgcaccacgc cgaagactca aagcagcctc ctcgaagcga tgagcgaatc
    23881 ccagatctcc gtcgacaacc aaacctatcc ccttcagcag ccgtttatgg tcatcgccac
    23941 acaaaacccg gcagagtatc acggcacctt cccgcttcct gaatcgcagc tcgaccgatt
    24001 cctcatgcga cttcgcatcg gctatccgac tccggatgag gaaaagaaag tgctggatcg
    24061 cccccagctg ctgcacccgg ccgacgaaat tcaaccggta ctgagcgcgc agcatatcgt
    24121 cgatcttcaa gcgcaggcag aaaaggtccg gatggaagac agcctgatgg attatcttct
    24181 cgccatcgtg ctcgccacca ggcacaacga gctgctgtca ttaggcgtga gtacccgagg
    24241 cgccctcgcg ctgtgcagag cggcgaaagc cctggccctc gtgcgcgacc gcgcctattg
    24301 cctccccgaa gacatcaaag agctcgcacc aaccgtcctc tcgcaccgtg tcatgctcag
    24361 tcggtcccag gggatgcgca cgaacaccgt cgaacatacg gaacaggtca ttcaagacat
    24421 tcttgagaca gtcccggttc ccgtgtaaca accgccaggg ggccgaacac cggcatcacg
    24481 cgaaatgtcg cagtaatgcg gtctggccgg cagcagtcca tcagcgtggc aaccaacaaa
    24541 ctccaggctc tgctccgccg gctgttccgg gaccgcacca tccgggtgac aacggaaggc
    24601 atccgtttcc tgctgttgac cctggccgtc gggatcgccg ccgtcaacac cggcaataat
    24661 ctgttctacc tcctgctggc catgatgctg agcctggtcg tgctgtccgg gttgctgtct
    24721 gaacaatgtg tgcggcgact ggacatccat cggcacctcc cggactacct cttcgtcaat
    24781 cagccgacca ccgcaacgct ccgggtcgcc aatcacaaat cacgtattcc aagtgtctcg
    24841 ttacgcctcc tcgatgtcgt tgccgggaaa gatctcgatc gggggattca catcacacac
    24901 ctcgcgcctg cagcctccac cctctgctcg tacccgctac tggtgcgaca gcgtggacca
    24961 taccggctcg acggaatccg tatcgtgacc ccgtttcctt tcggtctgtt ttacaagaaa
    25021 tccttctatc cactcgaagc cacactgatc gtctgtccgg aaatcattcc ccttccgccg
    25081 ctgcacctgc aggaactgaa taccctgggg caggatcacg cacgcgcgcg acgcggaccg
    25141 ggtaactccc tgtacaactt gcgcgaattc aggccggggg acgattcccg cgccattcat
    25201 tggatgacca cagcccgcac ctcgaagtta atgctcaagg aaaccgaggc ggaagagaga
    25261 cgatcgatca ctctcgccat ctcgaccgtt gcgccggacg aggcagagaa cagcttcgag
    25321 cgggcgctct cgatcggcgc ctccctcatt gatcactttc tgaaagacgg ataccaggtc
    25381 cggttgctac tgggcgatca gcaggacatt ctggcctgcg ggactgacca agccctgcat
    25441 ctatttcacg ccctggcgct ctgcgagcga ggcccgatgg caacgggagc tgcgatccga
    25501 cattccatgg cccgggcgct tgcagaattg aacgagggac cgaccattct actctcaccc
    25561 tggaccgatc ccgcccggaa tgagcaattt ccatcggtcg attacatcgt gtctcctcag
    25621 tcacacaggg atctcttcga tgacactgga tcgagccttt cggcttagct caattctgct
    25681 ggcggcaacc ggcttcgcga gcctgacgct tgcgattgcc ctgccattct ggcttctcgc
    25741 cttggcaggt gcagccttcg ccgtggcact cctgcgcgtg aatcacgccc cgggcttctc
    25801 cgaactcttc ccgcatctcc gcctctccgc cctgacatgg aatatttttc tcctcctcgc
    25861 ctttgcgggc ttctggatcg atctgttgct cgtctcgcag gaacttctcc cggccgggat
    25921 tcatttcctc gtactgctcc tggtgaacaa actttccaac ctcgaccagc ggcgggattt
    25981 tcttcacctc tatgccatta gcctcatcac actcctggcc tccgccgcgc tgaccacgca
    26041 aatctggtac gcccccttct tctttgtcta tctggtggcc ggagtctgga cgctgctcct
    26101 gtatcacctg ttgcaagagc acgaagagag caccggtcac catttgctcg gcagccaagc
    26161 tgcaccgctt gactcgacac ggccgcacat tacggcccgc ttcttttgga ctaccaatgc
    26221 gatggcagcc ggcgcgttcg gcctcacatt gttctttttc ttttcgatcc cacgggtagg
    26281 ggttggcctg ttgcaacatt cccatgggga aagcctgcga acgacgggct tttcagagca
    26341 ggtcgatctg ggtgtcatgg gaccggtgaa gcaggatccc agcattgtca tgcgcgtgga
    26401 gttgccggac agtcccggaa ctcccgcagc acgtgggccg ctgtatctcc gcggcgtcgc
    26461 ctacaatcgg tatgacggaa aatcctggag caacaacctg ctgcaccgcc gtatgctcac
    26521 ggaacttccc caaggcacct tcacgctccg aacaccgggc gccaaaccgc cgccccaggc
    26581 acgcgcactc cgacaagata ttctgctgga accgctcgat acggccgtgc tgttcggcgc
    26641 ccccctcgcc ttcgccgtca agggcgactt ccactcggtc caatccgatc tgatgggatc
    26701 gctgcacttg gcgtttcctt ctcatagccg catccagtac acggtctact ccgtaccgac
    26761 cagtctcaat ccggcggagc gggccgccgc cgcggtgctg tacccggagt ttattcttca
    26821 gcagtatcta cagattcccc ccgtcagccc gcagatcctt gacctcgcac agcatgtcac
    26881 gcagtcggcc accagcgtct cccaagccgt taccctgatc cacacgcact tgctggcgaa
    26941 ctatcgctac aacctggacg tgccctcgct ccaatcggct cacccgcttg aagactttct
    27001 cctgactcga aaaacaggat attgcgaaca ttacgctacc gcgatggtgg tgctgctccg
    27061 cgccaccggc attccggctc gactggtcac gggattcctt gcgactgaat ggaatgagtt
    27121 cggcgggtac tatacggttc gacaacggga tgcgcatgcc tgggtggagg tctatttccc
    27181 acggtcaggc tggattacga tggatccaac gccgcctgct ccagagaaca ttgggcaaac
    27241 ctggtggcaa tccgccggtc gtgtgatgga ttcggtccgg cttcaatggg atcgcctctt
    27301 tgtccacttc agcgcaaacg atcaactcac tgtcgtgcaa ggcattcgag agggcgggga
    27361 agccgttcgg acaaaactct ccgaatctct caactccctc tcggcccaga gctccgcgat
    27421 gttccggcgc ttcatgtcct cgttcacatc gaccggtatt ccggaaactg tactcttgat
    27481 cattctggcc atcggcgcga cgtatgcggg tcggcgactg ctgcggtcgt cccgaaacac
    27541 aacggcaccg ccggatgtgc tgtcagcgca tcaacgcacc gtcgtcacgc tctacaccgc
    27601 catgctgcac tgccttgcgc aacgcggcat cgtgaagtca gcgagcgcca cacccatgga
    27661 ggtgcttcgc catgttcgtg acgaatgggc tgaagcctgg ccctccgctg atgcccttac
    27721 acgactctat actcgagtcc gatttggtca cgctcccctc actcccgaag accataccgc
    27781 tgccgaaaac ctgctgcgca ggctccacac actggaacga gcgacaactc gttcacaaca
    27841 atagtcctga ctgaggccag atttcagcga ctcgacagag acaaacgcgt atgaacagga
    27901 gcggggaaaa acggcgtgct cggctggcaa gtccgcccgt caacatgaaa cctgtcaggg
    27961 cgacctaaat gatggataaa ggccggccag gattccagcc gaaagggacg aaccgtggtc
    28021 gtcgaaaacg atatgggtaa agatcgtgct gaggagaagt gtgaattgga gcgggaaacg
    28081 ggatttgaac ccgcgaccct cgccttggca attctgaaaa gagtaatttc gtaagggctt
    28141 gattgagcct tggtttcccc gattttccgt gatagtggct cggtttaggt gctgtctatt
    28201 aagtcgatag gttctggcgg gattgataga ttctgacgac ttctagcaca aatctagcac
    28261 acgaaatggg gtgaacccct tggtctgtag gagtcggaca agaatcagcg ttgacattgt
    28321 gagtccacct gaaccggggt catagtttcc tttgtgaaaa gcgcatcgat tgcctttcca
    28381 agaaaattgt actcgtcatt ccacgtgaac acgttgatcc agaatacggg tagaacaagt
    28441 atccaattaa aggtctttgt attaacgaga tatcgttccc tcagcaacgc tcgatcaggt
    28501 gcacagagtc ccaggacaaa ctcatacatt ccttcattcg ttgcccatgt ggggattgcg
    28561 cccaacgtga gaccggttac ccactcaagc cctgcccctc gcggctgact ctccgatact
    28621 tgaacatcga gtaaaatccg cgcaccgtcc tcacttgcta agtgaaagcc ggcgcgctca
    28681 atggcacttt cgatgttctt cctgaacttc gagtcagcct ccttatatac gaattctgcc
    28741 aaatgcgggg cacggtaggt taaacggtac gccaaaaatc tggcattgtt ctggtgatcc
    28801 gttgtcagtg atggaatttc tggcgaagta gtatgccaaa caatagtgca tccttggtca
    28861 tagagtagga cgagtaccaa gagcaccatt gtccattttg atcggatcac gacctgtctt
    28921 ctgcctttgc aggttcgctt tttctgagca ttgagattcg acactaagta ttttttacgc
    28981 cggcttgatc tcgcgaaggt ttccccgttt tttcaattga gcggttaacg tcagtctatg
    29041 aattctatta attggaacca atatgagcag ttttctagca cacatttagt acacgtccgt
    29101 ttgaactggt tgtctcttac tatctgatgt tgtaggaaaa cgggactaca aagactgttg
    29161 gcttatgagc atcagtactg tttagtggat atggggagca atccatgacc aattttgtag
    29221 cggtctgatc gagcacgtcc gatcctgaac tttcgtctaa ctttatttct tcgacttcac
    29281 cgttcgtcct caacgttacc cgcataatga cagtgccgct ttgagcggac gcaagcccta
    29341 tcaactgaga tggtttagct gcttgggcag taaggcaagc ccatacatta gcagcaatct
    29401 tacgttgatg ttcagtcata ggctgcaggt ggtatcgaaa aggaattaca gcggaatatt
    29461 ccgggcgatg gagatccaga tcaagattaa gccgcttcat tgattggata gcgtctagag
    29521 cattattgtc gagcaatgga atgccagagc tttggtgcac tttagcttcc gttatcgtac
    29581 cgtccttcaa gaaaactacg caaatgagta caactccctg ccagccacgt tgttgagcct
    29641 tagtaggata ggtcggttgt ggaatctgct gccaaaggta tgcccctatc accttgggat
    29701 ggtccactgg tagatgatct tccacattcg actcaggggc agtcggtcta cagggaggga
    29761 tatgagatat cgcttgtcca gcgccacttc taagggtgac aaccttaata ggagtgactt
    29821 ggtcacttgc acagccgata agcagggcgg tcaagaaaaa gacaaccaag gatatgtgct
    29881 tgcgagtcgg catcgattag tatccccggg aaaatcggtc gcaggatgaa cgaggctaag
    29941 tctaatcgtt ggcgtcggca agtgagtagt cctcagtggg atcagaaccc cacctgactg
    30001 gggggggtta caaaacaagc tgattagagg agtacggaag gaatttattt cggtaaccgg
    30061 gttgggcgtt tggccacttg aaatggaagc tctctcataa accaagcacg gccattgaca
    30121 atttatatgt actatattat gtaatgcata catatcatgg aggacgttat gaagggtgag
    30181 gatactaaga cagaaacgct gcagttcaag gtcacagaac aagagcggag gctgattgaa
    30241 agatgcgcgc aagaggaagg gacgaccgtc tcgaagtatg tgcgtggggc agtgctcatg
    30301 agcatggtga tggatggcaa ggctgaggcc atcaagatcg tggcgagaga ggtcggagag
    30361 aaggcgtttg cggtggtacg ccagaaactg acccgaccgg cacaggaagg acgctgaaaa
    30421 tagtgctatc cgtcagtcca tcaggaggca aagccggtcc gcgccgcatg ccggcaggct
    30481 gcggggcgga gcggaatggc ccccgtcttg gtaacacggg ggcgctaccc acagcgctcc
    30541 gtacaggagt tcgatagatt ccatggattc tggcttcacc ctcaccattg actggctggc
    30601 cttcacggtc ttggcgagca atcccgaaga gaccatgcgg gtccttggtg gagactggag
    30661 caaggctaag ggcgggttcc gaggctatcc cttgtcctgg atgagaagcg acggtctgcg
    30721 tggggtcggc aaattgggca ccaatgcccc tcgtcgtccg aatgaaatcc atgtggatct
    30781 gtcgggcggc ctggcgtccg ccctgacact ggatcaaatc cgaaccctcc tccagtgggt
    30841 gcataagcag caaggacacg tcacgcgcat tgattgcgcc ctggatgacc gggcgggaac
    30901 ggtcccggtc aggacgattc gagaggcggt tgctgcaggc cagtgcgtca cgagggcggc
    30961 tcaagtccgg catatcgtct caaacctgac gcatgggacc ggagcgacga cgggtgagac
    31021 gatgtatttc gggagtcctc agagtcagac cttattgcgc atttacgaca aacggctgga
    31081 acttcagagc aaaggacaag agaactggcc ggagtacggg actcggtggg aattagagct
    31141 caaaaaggat cgcgccgaac agtgcgcaag ggcactggca acgttagacg aaaccgactg
    31201 gaaggagtta gtcattgggt tgctccggtc ctatgtggat ttccggcaga cttcaaagga
    31261 tgccgaggat gaagaacgct accgggctcc agtgctggag tggtacgccc tcctgacgga
    31321 gggatttcag aaggggcgat tggcccagga gcagcaggtg cagaccctgc aaaatgtgaa
    31381 acgatgggtg agtgacaccc tgacgccgat gttggcggtc atttgtgcca cccctggagg
    31441 ggaagaatgg ctactggaag aaattgtcag gggcatctcc aggtggaagg accgacaccg
    31501 gaacttgctc aaacagcccg ctcggtttca ccggtctgcc ggcggtcacg cgggcagccc
    31561 atgttagggg gactgggggt gtcgagagac tcccccggtg tatctgcaca tgctcaacgt
    31621 caaagagctc tcggcttggc tcaatatcaa gccatccaca ctctatctct gggcctcaga
    31681 gaacaagatt ccctgccggc gcattcacgg tctcgtccgc tttgaacctg aggcgattca
    31741 ggcctggctg aagggctttg agaccaattc cgtcaagccg ttctctctgc cgactcgcca
    31801 ctacacccgt gaccttgacc agctcattga agcctcaaaa cgcgaagtct atacttcccg
    31861 tcacggggaa accattcagt caagcctcat ccagaaggag gatgcagatg gggctgttta
    31921 gacgcaataa agtatggtgg atgacgttca ttcatcaggg ccagcaagta cgacggtcca
    31981 cagggacaac ggacaagcgc ctcgcagaag cgattctcgg gaaagtacgg gtcaagattg
    32041 ttgagggccg attctttgac acgttggaag ccaaagacca tacgttcaac gacatgatgg
    32101 atcgatacct gaaagagcga tcaatcacca aagccccggc aagccgacgg cgggacgagc
    32161 agtgcctcac gcatttacga agtgcgtttg gctccttgaa tcttgcagaa gtcacaccga
    32221 aacttcttgc cgcctacaag gcgaagaggc gtgagacggc caagccagcc acggtcaata
    32281 aagaactcgg cttggttcgg catagcttca acgtggctat ccgagagtgg gaatggtgtc
    32341 gagacaatcc catgcagaga gtctgcatgg agaaagtgcg caacgagcgc gaccggtggc
    32401 tgtcctcgga tgaagagggc cgattactgg ctcattcggc accgtggctg caggacatca
    32461 ttgtctttgc cttaaacacc ggcatgcggc gaggcgagat tctcaacttg caatggcaag
    32521 ccatagacct caatcggcag gtcctggtgg tgatgcgaag caagaatggc gagaagcgga
    32581 ctgtcccgct caacggtcgg ttaacggact tgctgaagag aaaagcgtca aagagggcag
    32641 caggctcgtt cgtgtttgcg agttcagctg agacgctatt cgacgctcga aacctcacgc
    32701 gatcattcag actcgcggca acgaaggcag cgttgagcga tttccgcttt cacgatttga
    32761 gacacacctt tgccactcgg ctcgcacaag ctggtgtcga tttgtacaag gtgcaacgtc
    32821 tgttggggca caaaacagca gctatgacgc agcgctacgc gcatcattcg ccggagagtc
    32881 taagagaggg agtgttgatt ctggagcgcg ttcaccctca gatatcacaa aactatcaca
    32941 atgagggtgg agccgtcgga gagtgttgtg caagtgcttg agaaaattgg agcgggaaac
    33001 gggatttgaa cccgcgaccc tcgccttggc aaggcgatgc tctaccactg agctattccc
    33061 gcttccaccg acagaagaag cgggattcta ccggccatgc gtaaagactg tcaagccggg
    33121 cagcagaaat atcgtcatga cagcgactct acgtagctgc ctacgcgggg ccactgtcaa
    33181 agccggccat ctgcacgaag cgcgggtggc cccttttctc gcaaacatac agcctttcga
    33241 ctcagacttg cgtgcttctt ctgtccactt gacggacctg cgaggcttga gtagagtgat
    33301 tccaatgtcg gcccactcca tcctcatcag cgaggtaccc ttagcgtgac ctcacgtcct
    33361 cagccgtccg ctccttttgc agcacaagcc attcccttcg acgagtatct cgcatcgggc
    33421 aagatccccg aggggcttct cacgagtgaa tacatcgggc aacagtttgt tgagcgattg
    33481 gttcactacg tactgagcgt tcctgcaggg agctacacta tggcacaact cagccgtctt
    33541 ctcgaggagc tggatccacg cgcccaggtc ttctttttca agcgactgaa agaaaatagt
    33601 ccggatagtc tgaaagattt tgccccgctg tattacggat tcatgaacga gtttcattcc
    33661 ctcctgttta cgtaagtcac tgcccagtaa aaaaatacct tgtcggcccc ccattctcag
    33721 gtataatcac gacaacctct taatgagcca aggagcacta tgaatcccac gttacagcgc
    33781 gctgttacga gtttttataa tctggtgtac gaagcccaga ctttcgcgac caccatgtct
    33841 cggatcgaca ctgccgagca ggagcactat gccgggcgga tcgaggggct gaactgggtc
    33901 ctggaccgct gtcaggagct tgaggacatg gatgccaaca tcactcctac gacactgcaa
    33961 cgtgtcctga cggaagtgaa atcggacttg gatcatgagc tctccgtgca gcgtcgcgaa
    34021 aaaggccgac gggccgacgg acgcgaggaa gccctgacct tcgtggcgga ctacttgtcc
    34081 agcctgatca cggcaactga gatcgaatcc gccaaaacct ccgtgtaatc gcctgtcgat
    34141 tgtctccagg cttatccttc gtcatggcct cctccgaggg cgactatggc ccgtgagtgg
    34201 atcatcttcg ccgtttgtct cggcttggga ggccatattg ccctcgccgt ggtgttgcac
    34261 gcgcctgact tgtggccatg gagcaaagcc tggctctacg ggttgctctc gggcgtcacg
    34321 ctctatgtcg tggttcaggt catccgttca ctctggtgga tttttcgcgg caggaacgcc
    34381 gaggagccgg aaacacccga ggatcagcgt atcggccatc cttgattcca atcaggcagg
    34441 catcgtcctg ttgcaccaca tccatctccc tcagtagaat accgctcatg cagccacgtt
    34501 cgaacagacc cgcgccgccc aattggcctc caagcgccat gcgaccgcgc cccgcaccgt
    34561 cacagctagg gcaacgccag acagatcaat acgactcgtt taacgcctcc ctgctgtact
    34621 gcggacaatg ccgccaggca actcctacac gtcaacggct cctgctcgtt ctgccgacag
    34681 gcaatctcta cgaatacttc tgtgctcaat gcggagcctc aaccggctca aagacggaaa
    34741 gtgcttcggc agaaggtgga ctgctcgccc cgtaagcata cgtgccgcaa tccactgtca
    34801 aacggtcact ccagcacgtg cgttcaccca taacacgccg aaccttacgc ctggggcatt
    34861 actactccat gacgaagcgc gaacgcctgc caagagagta ctcgcattgg ggaagagagg
    34921 accgagggag ggtaatcagg aaacctgatc gagagggata ccttcgtcga tcaacatgac
    34981 aggaatgtct tctcgtacgg ggtagagaat cttgttgtcg gatcggatca atcctccatc
    35041 gagcttctcc gtaaccggct tgtgggcttt atttttgagc gccccgcgct cgatcgcacc
    35101 gttcaccttt tgaatcaact gctcgtcggc aagagccacg gcctgcttcg tttcggggca
    35161 acaaagaatg gctaacagat ccttgtcgag attcaccttt tttcctgcgg catcgctcac
    35221 aatatctctc acaatcccac ccgatagggg tgactgaacg gttaccggaa agatagtcca
    35281 aatgaatcga cagatcaaga agataccaaa tcgtccccct ttctgatatc gtcattgatc
    35341 tattccccct tcactcacca acatcggggc gcgacgtgac tgcttcgcat cggctcggac
    35401 gcattttcct taccggactc ctcgtgctcc ttccggcttg gacaacgttt ctcattttgg
    35461 ccgcgctgtt cgagacactg gattccttct tgctcaatct catcggccgg cagatccagc
    35521 cctatgcccc gggactcggc ctcctgctgc tcattggaat ggtactcacg gtcggggcca
    35581 ttgcgaccca ggtcatcgga caacgagttg tacgctggac cgaggccgtc ctggaccgga
    35641 ttcccttgat tcgcagcatc tatatgacac tcaagggtat gacggattta ctgaactacc
    35701 gggcacgctt cggacaaagc accgtggtgg ccttcccttt tccacgcgac ggcctttggg
    35761 ccctgggttt tgtcatgggc tctccaccgg cggcacttca aatcgcaccg atggtggaat
    35821 tggtgatggt ctttgtcccg actgccatcc atcccttcac gggatacttg gccttgattc
    35881 ccaaaacaca attacacccg ctgaacctgt tgccggaaga ggcattgaag ttggagtttt
    35941 cagcgggact ctatcgcccc ttaccaggct ggctgacgtc gccggcacgt aacccatcat
    36001 gagtctagcc gatagcctga ccgtacgtcc tgccacactg gaagacctcg acgcgctcac
    36061 gacattcagc gccgccatgg ccctggaaac tgagcaacgc acactggatc acgcacgctt
    36121 acgcctggga acccgagcgg ttctcgaaca gcctgaacga gggcggtttt acgttgcaga
    36181 cttgcgacca gagccgccag ccgatagagt aaccgtaggc cagctgctcc tcacgtacga
    36241 gtggagcgat tggcggaacg cccaattctg gtggatccag agtgtgtacg tacaccctga
    36301 ctggcgacgc aagggcattt atcggtcgat gcatggcgcc atcatcaacc tcgctcgatc
    36361 acaaccgggt gtgtgcggtg tccgcctgta cgtcgaaggc gacaatcaca tcgccaaatg
    36421 cgtctatgaa cgagtcgggc tctcgccatc cagctaccag gtgtacgagt ctgattttgt
    36481 cctacccacc ccccacagcg cccgcacaaa ggcgtaagtt agtccgatcg acgtccgcat
    36541 aaggaggatc acatgcgacg agtgagtgtg ctgttggttc tgatgctcct gacactgaat
    36601 gcctgcgcgt tcagccgggg caccctcggc gacgagatca agaccgaaac cgtcaactcc
    36661 atcaagaagg gagtgaccac caggtcggaa gtcctggctc tactcggtgc tcctgaccga
    36721 ctccttcaac tgaacggacg agatctcttt cagtactacc ggtacgatgc caaggtcggc
    36781 agtctgctgt tgatcgtgct caatttttcg cgcgtgtcgg tcaagagcga cgatctgttt
    36841 gtggtgatca accgcgaagg gaccgtcgag gaagtaatcg cgtcaaaacg aacagagggc
    36901 ctggaattcc gtttctggcc attcggagac taaggtcatg acaggctgga accgctctgt
    36961 cacgctgctg ttctccgcag tcattgccct ggctttgtcc ggctgtaacg tggctcgcct
    37021 cacgatcaac acccccctga cgccggaatc ggtagccttc attgcggaag gccgcaccac
    37081 actggccgat gtcgtcgaca cactcggagc accggattcc attaccgata cggaaacagg
    37141 aactatcgtc acctaccggt tcctcaacgc caaatattcg cgggtcaatt tcggctggct
    37201 actgaaaccc tggtcgcccg tggatcccga cctcgtgttc tcgcgctccg gcctcggcac
    37261 agacgcgttc gaactcctgt gcgacgacaa gtggattgtc acccaccatg cctttgtgcg
    37321 tcaccgcccc gcaccgccgt tcacacccta tccgttctga cccctctgca catacgcctc
    37381 gcatcggtga attgccgccc ccggcacatc tcgttataat gccgctcatg ggtgcaggtt
    37441 cttccgacga gtcatcgagc caacaggccc ccggcacatc atcctcctat atcccggctg
    37501 atcacgacat cgactttctc cagcgcgacg aactgcgccc cctgcgcctc gggctggagt
    37561 tgctcaagcc ggaattgata cagacagagc agggtatccg ctcgacgatc gtggtcttcg
    37621 ggagcgcacg gctgctggag ccatcgcgcg cacgccaagc cctggaacaa gcctcggcag
    37681 cattgcaggc ctgtcccgac gatcgaacct gtcagcaacg tgtcgccgta gccgaacgcc
    37741 gcctggccct ctcgcgatac tatgatgtcg cccgggaatt cggccggctt gtgtcctcaa
    37801 cctgccagat cgacgggcaa tgcgactacg tcatcgtcac gggtggaggc ccgggcatta
    37861 tggaggcggc caatcgtgga gccgcggacg caggcgccaa gtccatgggg ctcaacatca
    37921 cgctgccgca cgagcagcgg cccaatccct acatcacgcc agacctctgt tttcaatttc
    37981 ggtacttcgc gctgcgcaag atgcattttc tccttcgagc ccgggcgctg gtcgtattcc
    38041 ccggtgggtt cggtacactc gacgaactct ttgagacact gacccttctc cagactggca
    38101 aaacgcgcaa catcacgatc atcctgatgg ggcaagcctt ttgggagcgc ctcatcaatt
    38161 ggccgctgtt gattgaagaa ggcctggtgg gccccgagga cctcgcgctc ttccactttg
    38221 cagaaacggc tcagcaggcc tgggatctca tcagccgaag ccatggaggc ccgtccgccc
    38281 gatgaaactc tcattccacg gagccgcgag atccgttacg ggaagccgcc acctgctcga
    38341 aatgcccggc aatagaattc tcctagattg cggactcttt cagggacagc gccaggaagc
    38401 ggatcgtcaa aaccggtttc tgggattcga cccgcgctcg atccaagccg tgttgttatc
    38461 ccatgcccat atcgaccatt ccggggctct tcccgtgctg ggcaaacatc agttccgcgg
    38521 gaatgtgcac atgacccgcg cgaccgtaga tcttgccgcg gtgatgctgg aggattcggc
    38581 ccgcctacag gagaacgatt gcgcctatct gaacaagaaa gaaaaccgtc ggggcaagcg
    38641 ctgcctgcaa cccttctatg agagccgcga tgcccgcgcc atcatccgtc gcttcgtagg
    38701 ggcgcggtac ggggacacga tcaaggtcac ccctcgcgtc accgcctcct ttcacgacgt
    38761 cggtcacatt ctgggatccg ccgccattcg cctcaaatat tccgcgcgcg gcaacagtac
    38821 gacagtgctc ttctcaggtg acttgggacg gtcgcagatg ccgatcctcc gcgatccggc
    38881 tccgccgcca agctgcgatg tgctgatcat tgaatccacc tacggcgacc gcctgcacga
    38941 agaagccggg gaggcgctga cccaaaaagc ccagcaactg gtcgcccatg ccaaggcgca
    39001 caaaagcaag atcatcgtgc ccgcgttcgc gctcggacgc acgcaagacc tggtcatgcg
    39061 gatcaaacga ctggtggcgg aggggcgaat cgacccgctc ccgatttata tcgattcgcc
    39121 tctggcctcg aaagtgacag acgtcttccg aaagcacccc gagtgttacg acgaagagac
    39181 cttccgcacg ttcacatcgg aaggggatcc ctttgcggcc cgatatatcc gctatgtctc
    39241 ctcgccggaa gaaagcaaac gattgaatga gatgaaaggc ccctgcgtga tcatcgcctc
    39301 ttccggcatg tgcgaaggag ggcgcgtcgt gcaccatctc aaacatggca tccaggacga
    39361 agccaacatc atcgccatcg tgggatttca ggccgagcat acgctggggc gcagactggt
    39421 tgaaggatgg gatgtcgtgc cgattttcgg gatcccgacg ccgcgccgtg ctcaggtggt
    39481 cgtctttaac ggattgtcgg cccatgcgga ccggaacgat ctgcttgcgt acgtgcgggc
    39541 gatctctccc gcccctcagc aagtgttcgt ggtccacgga gaagaaaaac aagccttgtc
    39601 gctgggagcg gccatccaga cagaacaccc cgggatggct gtcgtggtcc cggccaaaga
    39661 cagcatatac gagatctagc cccgctgctc gggcacacct actcagggag gagatcggca
    39721 gtcatgaacc cgtcaagaac gattctcaat gcggtacggg cttgcgccgt gattcttctg
    39781 actgcctgcg tgtctccggc ctggtgccta gacggatcgc ccgattcggt ggaccaggtc
    39841 cgaatccgcg cgcgcgcatt ggggctgcac cccggacaat ttcagcccgg tcccttgaat
    39901 gccatcaccg atgtgcccgg ggtcaaggtc ggccaggtga cgctgatgca aggggacggc
    39961 cccctgcagc ccggacaggg accggttaga accggcgtca cggttatcgt gccacgggat
    40021 gatgtgtggc acaagaaagt cccggccgga gcctttgtcc tgaacggcac cggagaaatg
    40081 acggggctct cctgggtcgc agaatcgggc tttctcgaat accccatcgc actgaccaac
    40141 acactgaacg tgcctcgagt tgccaatggc gtgatcagct ggatgctcaa gcaatatccc
    40201 ggcatcggca ttaccgacga caccctgacc cccgtcgtgg cggaatgtga cgatggacgc
    40261 ttgaatgaca ttcaagggcg tcacgtctcg gaaaccgatg tcatgcgggc cttggatgaa
    40321 gcctcctcag gccctgtggc ggaaggcagc gtgggagccg gaaccggcat gatctcctat
    40381 ggattcaaag gcggcatcgg cacagcctcg cggcggctcc ctgaggccaa tggcggattt
    40441 acgatcgggg tgttggtgaa cgccaaccat ggccgcagac cagaactcac catgggcggg
    40501 gtaccggtcg ggcaacggta cggtgccgct ctaacccaat ccagcctgtc gccggaacag
    40561 aattcgagct cccttcacgc ctcccgcgaa ggaaccagcg gcaacgccga agggtcgatc
    40621 attgtcgtca ttgccaccga tgcgccgttg gatggtcggc aactcacccg cctcggaaaa
    40681 cgagcggcat taggactcgc ccgcaccggc tcaaccgccc gccacgggag cggggatttc
    40741 atgctggcct tctccaccgg caacgtcatc ccccattacc cgtccgaccc caccttttca
    40801 ctcacccatc tggccgacac ccacctcaat ccggtgatga ccgccacggt tgaggccaca
    40861 gaagaagcga ttctgaatgc gctcaccgga gccaccaccg tcaccgggcg cgacgggttt
    40921 cgagccgaag ccatctccat ccctcgtctg cgtgagctcc tatccgccgg tgccacgccc
    40981 gggccctaac agcccgccat ctccccggct gagccgtcga taccttgctt ttgcaaggcc
    41041 ccacggctat tctcctggct tgtcagtacg tcatccggag ggctatggct gattatccta
    41101 tcggaggtgg cctcacactt ctgaccgcgc tcgaaaagaa cggcaagttc gctgaatttt
    41161 tacagacgcg catgacgcaa gcactggaaa cccaggaccc gaccgaactc cactacttgc
    41221 tcgcccagct cgatgactac cattcgtata tgtggcgcta ttacaagaaa ctcgcgtcgg
    41281 accgacctga gcgcatgaat ccgggtgtct aaccgcaccg gacagctcac gaccctgatc
    41341 ctattggcaa agacagacct gtgattctca cacctccatc aacactgcgg ttcgcctccg
    41401 ccctcacccg tcaggctgac gcgcaggctg cggcggatga attgatccga gccattcgcg
    41461 tgcaattggg ctcctcacgc atcgacgtcg ccttcctgtt catctccgcc caacataccg
    41521 atcaggccga tacgctctcg cacgcactcc gcacggcact cggccccgac accttggtcg
    41581 gttgtacagg agagggagtc attgccaccg gacgagaagt cgaaacggga ccggccgcaa
    41641 ccctctgggc ggcccatttg cccggagtca tcgcacatcc gttacgactg tcgttttcaa
    41701 gcgtccacga tcagttttca ctgcgtgact ggcccgaact ggactatgga ggcgagtcgg
    41761 cccccgtcat gctcctcttc gcagatccct tctccacccc gctgcaagac gtgttgggcg
    41821 tcattgagga gcgttacccg catgctcgtg cgctcggtgg gctggcaggt ggcgggcagg
    41881 atcttgccga gaatcgcctt tttctggatg acgaggtgta cagcgatggc ttggtcggtg
    41941 tcgcgctctc cggaaacatc tcggttcgca ccgttatttc gcaaggctgt cgtccgatcg
    42001 gagaccggtt catcgtcaca aaggcggagc acaacgtcat tcaagaactc ggcggaatcc
    42061 cggccttgca ttgtttgcaa accgtcttcg ggcaactctc catggatgaa cgcgcgcaag
    42121 cccaacgggc cctccatatc ggcatcgcca tggatgagca acgcgcgcaa tttacccggg
    42181 gagattttct gatccgcaat ctacttggag cagaccagca gacgggggcc atcgtcgtcg
    42241 gtgacgtcgt ccaggaagga caaaccgttc aatttcaagt cagggacgca cagtccgcag
    42301 acgaggatct ccacgcgtta ctcgccgctt cccgtctgga ggaatcacaa cggccactgg
    42361 gggcgctgct gttcagttgt tgcggacgcg ggaaaggact cttcggggtt cccaaccacg
    42421 acgcctctgt cctgggggaa caattgggcg ctattcctct ggcgggattt ttcgcgcagg
    42481 gcgagttggg accggtcggc ggacgtaatt ttctccacgg ttatacggcg agcatcgcca
    42541 tcttttctga accggaccga tcagacacac aaggctcgaa tcattgacta ccgtcagcgc
    42601 atgcaggtta cgccaccgga cctgcagact ggacaaggac acctctatga acactcgctt
    42661 ctggctgacg acttgcggta tcgtgggatt cttattcctg accataggga gaccaaccat
    42721 ggcacagagt gaacaaaccg cctcaacgcc tgaagtcaca accgtttcag gccttaccta
    42781 cacagacgtc gtagtgggca caggccgtga ggctgccgcg ggaaacctgg tgaccgtcca
    42841 ctataccggc tggctgacca acgggaaaaa attcgatagc tccgtcgacc gcagcgagcc
    42901 gttctccttt ccactcggtg ccggcagggt catcaaggga tgggatgaag gcgtcgcggg
    42961 aatgaaagtc ggcggaaaac ggaagctgac cattccttcg caacttggat acggcgcgcg
    43021 tggagccggc ggcgtgatcc cgcctaatgc gaccctcgtc ttcgacgtgg agctgctgga
    43081 agtccggtag gaccgtttta cacgcgcagg cattcactca ccaccgtgga cgttagacgc
    43141 acctgatgaa acagacaccc ctcatcgacg ctcaccgcaa agcccagggc aaactcgtcg
    43201 actttgcagg ctgggaaatg cccatccaat attccggcgt agtggacgaa tatcagaccg
    43261 tccgccggca cgcaggactg ttcgatgtga gtcacatggg gcgcatcagc gtcagcggac
    43321 cgggctcgtt ggcatttttg cagcgcgtca ccacgaacga tgtgtcaaaa ctgtccgtgc
    43381 gtcaatcgca ttactccatg atctgtgccc cgaacggcgg catcaaagac gacatcttcg
    43441 tctaccacgt gaagccgtat gagtttttgg tttgcgtgaa cgcctcgaac cgggaaaaga
    43501 ttgtggcatg gctgcatgag aaggtggcgc aagcccaggg ctgcaaagtc caggaccagt
    43561 cagcgtccct cgcgcaaatc gcgatccaag gtccggcctc acgcgacatc ctggcaaccg
    43621 tgggccttgc cgacctcagc acgctgaaaa ttcgtcactg cctggacgcc accctatgcg
    43681 gtcacgcaac cttggtgaca cggacgggat acaccggcga gttgggatat gagttgtatc
    43741 ttccggcagc gggggcgccg aacgtctggg atcgactcct cgaagcggga cgtccactgg
    43801 ggattaagcc ggcagggtta ggtgcacgtg atttgctccg gctcgaaatg gcctacctgc
    43861 tctatggcaa cgacatgaat gaggaaacaa ctcccatcga ggccggggcg gaatgggttg
    43921 tgaaattcga taagggagac ttcatcggcc ggactgaatt gcttgcccaa caatcacagg
    43981 gagcagcacg acggctcgtg gcgttcgagc tcgtcgaaaa ggctgtgccg cgtcacggat
    44041 tcaaaattct aagtgcacag gtacctcatg ctgaaatcgg tgaagtcacc agcggaaacc
    44101 tgtcgccctt gctgcaaaaa ggtatcggta tggggtatgt cacgcccgct gcagcccaac
    44161 cagggtcgtc cattctgatc gatattcgag gcaagacttg ccccgccgtc gtcgtgaaac
    44221 caccgtttta caagaaaccc gcgggcacgc cgtaagtcat ggtgaaaccg atctacaagg
    44281 gcacggtggt cacgctcaac gtcgatacgg tgcgcctgcc gaacggccat acaatcgacc
    44341 tggaagtcat ccgccatccc ggtgcctccg ccgtcgttcc cctgaaagaa gatggaacgg
    44401 tcgtgctgat ccgtcaattc cgtcatgcgg cgaacggttt tatttacgaa atacccgccg
    44461 ggaaactcca tccgaaagaa gacccgctcg actgtgcggc ccgcgaactc gaagaggaga
    44521 tcggctacaa ggccggtcgc ttcgaactcc tctccagcat tttcaccgcg ccgggatttg
    44581 cggatgaagt catccacatc tatcttgcga cgggcctgac gatcggcacg caaaatctgg
    44641 atcaggatga agtcctggaa gtggtggaaa tgcctctccg cgaggccatt gcgaaaatcg
    44701 aggatggaac cattcgggac ggaaaaacga tcgtagggct acaagcagtc tatattcggc
    44761 aggagcaata ccggtagacg acccaagagg tctcccacct ccaggatggg agacctccca
    44821 agccagctag cctcgccaga aacggtgttg tgtttagtgg ccgccctttt tctcgtcctt
    44881 cttcttctct tcgccgaaca cggtctggga caggtgtcca cccttcttct cttccttctt
    44941 cttctcttca ccgaagaggg tctgggagaa gtggccgccc ttcttctctt ccttcttctt
    45001 gtcgtcaccg gcgaacgaag gagcgctgaa cgccaccaac aatgcagccg ccatcaccgt
    45061 caacatcatc ttcttcattc tgactcacct cctgttaggt ttgaaattac caacgaccga
    45121 cggatgttcg gtaaagcaaa gtccttgcct catacaccca cagatctaaa ctgttgtttt
    45181 ttcggacatg tcatgcatcg cacttcgtaa tgctacagaa agcacagctc gattctgtgg
    45241 cagaatagcc tggacgatac taaaccgcct catcagtcga ttcagcagac gcgacaaggt
    45301 acgctctcac gaccaccatg ccagaacaac ctcctctgcc agatcatctc cgcccggacc
    45361 tcagcatcgt atttgtgggc atcaatcctg ggctacgctc agccgcgctg gggcatcact
    45421 acgcggggcc ttcgaatcgt ttttggaaac tgatttacga cgcgaacctt gtgccggaac
    45481 cactcagcta ccgagatgac gggcgactac cggaatgggg attgggactc accaatctgg
    45541 tcagccgatc gaccgcgagc atggagagtc tcacgccgca ggattatacg gccggccgac
    45601 ttgtgctcat gcggaaactt ctccggtatc gcccgcgtgt tgtcgccctg ctcggcatga
    45661 cgctctaccc catcctcttt ccactcgaac caaaacagac aggacgaaaa ccaggtctgc
    45721 aaccagcgac actgtacgac tccaatatcg tgttactccc caacccgagt gggcgaaacg
    45781 catcctatcc ctaccattcc atgctggagg cttttcgtac gctgacaccg tgggccaaga
    45841 gcggcctcaa gagtcctccc tgacttatcc tattcacgcc atgcgctccg ctggaattcc
    45901 ctcggggacc tgctgtaaaa tctgcggctg atttccttcg ggcacaccga aacggacact
    45961 ccatgcaatg ttttcccatc acgatgagcc ttgcgatcgc tctgttattc gttgccgagc
    46021 cggcttccgc aggcgcctcg gttcaacaac tcacggccgg tgcgctgcac gaatgtgcgc
    46081 tgggccgtca ggctcagact cgtgcggagc gcgttcaaca tttcgcgcaa ggtcaagcgt
    46141 ttggagaaca ggcggtcgaa aaagacgact tatcgcccga tgcccacttc tcgctgtttt
    46201 gtaacctcgg tgagcaattg cggatcgacg gggaaagcct gacctcgctc ttcggcttcc
    46261 gacgcatgat gaaggagttg aaccgcaccc tcgaactggc tccggaccat ttggatgccc
    46321 tctcagccaa gggaaccgtt ctggtgcgca ttccggcatt catgggcggg gataaagaaa
    46381 agggggagac gctgctgcgt caggtcattg ccagggaacc ggctgccgtc aatgctcgat
    46441 tgagcctggc aaagagctat tgcgccaatg ggcggcatga agaagcggtt gccattgcca
    46501 caaaggcgct ggatctcgcg caaagcctgc agcgcgcgga cttcattccc gaagcccggc
    46561 aagtgctgtc ccagttacga gcccaagcgg ccaaaggtaa ttgagcagcc cgcttttccc
    46621 actcgcctga aatcaggcct accgacttac gaacgcggcg gcggttccag ctgcctgatc
    46681 ccggatcgac ataactgctc gatgtcatct ttggtcggca tcgactcctg cgccccacga
    46741 ccggtggtgg ccaacgcacc ggccgcatta gcccgcacga tcgcgctctt gatcggtacc
    46801 ccttcagcca cagcacaggc caaagcaccg ttaaacgcat ctccggcgcc tgtcgaatca
    46861 atcgtctcga caagaaaacc ggggatatgg gtcacatctt ttttaccaac cgtcaggaac
    46921 gccccgtttg cgccacaggt cacgaccacg gtgccgacgc cacgatcagt gagaatctgt
    46981 gccgcctctg ccggatccgt cgaaccggtc aacacctgcg cttcgctttc gttgggagtg
    47041 aggatatcga ccaatcgcag gaggtccgac ggcaatggag cagccggagc cggattaaga
    47101 atcgtggtga gaccgcattc gcggccaagc ctcaacgctt cgaaaacagt ctcccgcgga
    47161 atttccattt gaagcagcaa cacccgtgct tcggctatca actcacgatg ctgccgcaga
    47221 tcagctggtg tcagacgccc attgctcccc ggcacgaccg caatctgatt ctctccggag
    47281 agatcgacga gaatcatcgc aactccggtg gggaattcgg catcgcgaag aaggatcggg
    47341 cgcgacagcc cctgcgcggc caagtgctgt tcgatccacc gtccattggc atccgccccc
    47401 agcttgctga gaaatgcgac ctccgctccg gcgcgggctg ccgccacggc ctgattcgcg
    47461 cccttgccgc cgaatgattg ggcgaaacgg tacccgagga cggtttctcc ccggctcggc
    47521 aggcgatcga ccgacgccac caaatcgata ttgctggacc cgaccactag gatcatgggc
    47581 acagccggtt gcgtaggaaa ctcagcgtcc ggtcgacatc gacatcaacg gccacgcgca
    47641 tgttcggacg cgccttctcc tccggcgtgc gcgggcgacg atcggcgatg gtaatcccgc
    47701 gagacactcg cccggccatt tcaaccgaca catgcaatgg ctccaccttc aacaaggagg
    47761 aatcgacggc ggccagaacg gccaacggat cgtggaaata gaagagccca tgcccttcaa
    47821 ccttttcagc gaactcgaat gccttccgcg tcatgtcagt tgtcatccga gcgagcgggg
    47881 cacgcgactc agcggcccag gtcatcaagc tctcccgtgt cactccgacg cgggtcgtca
    47941 catcgagcgg aacgagcgtg agcgggagcg acgcctgaaa cacccgatgc gccgcatgcg
    48001 gatccacata catattaaac tctgctgcgg gggcaacatt gccgggtacg ccgatcgcgc
    48061 cccccatcac gatcaccgaa cggaacttct gaaccgtcag gggattcacc ttcaacgcca
    48121 cggccacatt cgtcagtggt cctaacgtga tcagcgtgac ctcatcggga tgccggcgga
    48181 cacattcatt ccaaacatcc tgggcagttg agagcacaga aggcaaccgg acttgagggt
    48241 atcgcggcgt cccctcctcg gccaggacag agtcgagttc gccaagcccg tcgcttccgt
    48301 ggacctggac cgctgtcaca agatcctcct ccaaaggccg cgccgcgccc tgcccgacca
    48361 acagccccgg aggtggctgt acaacgttca gcaggcggaa caaattcttc gtcgcctgtc
    48421 ccaccggcac gttcccgcac actgtggtca ccccgacgac ctgcaattcg ggcgaggcga
    48481 acgccagcaa cagcgccagg gcgtcatcga ctcctggatc ggtatcgaga atcactttcg
    48541 taggagaggg tctctgcatg cctagaagat ttgataggga atcgacccct attacaagcc
    48601 gacgtcacgc ccggataccg gtgcggcaaa tcgggtagct tgtccaagtc cttccagaac
    48661 ggactgatac cctgacgcct cgtcgggagg tcccatgaga aaatatatca ccgacgtatt
    48721 gccgggcccg aggactgcca aggcacgacc agccaggtct tcaccctgat acgagcgggc
    48781 gatcgagagt tcagtctcaa cttcagagcc ggcaggaata aacgacaacc cgtgtaacag
    48841 cgacaggggt tgactgaggt gatcgcggac cgtctcccgg gtcacatcgg tgaggggaaa
    48901 catcattccc aggcccttcc cttcttccgc catcagaaag ggcatctccg aatcttcggg
    48961 gcgagtactt tgccaaccgg tcggcaccag gaacgaccaa tcagtcccgg gaattctgag
    49021 cctggcgccg gcgcggtacg tcacgccggg atcgacatcg agcatgggac tgctctgttg
    49081 catctccgca gccgcgcccg attctgaatc agcccaagcc ggtggcgaga agaacagggg
    49141 caacagaaga caaccggcgg cgatcgaaag gacggcatgc acctgcggca ggcgatatgt
    49201 cgccgtgtcc gtcgaggagg caccgcccac agatcgatta ggaagcggga tccatcggag
    49261 gacaggtgca aaagaggtgg cggtcgccat aggcttcatc gatacgaccc acgctcggcc
    49321 aaaatttgtt gtcacgcacc cacggagcag gaaacgccgc ctgctcacgc gagtacggct
    49381 tcgtccactc agccgcggtc acagataatg ccgtgtgagg agcatttttt agaacattgc
    49441 ccgcacgcgg ttgccggcct tcggcaatcg attgaatctc gccccggatc gcgatcaagg
    49501 cttcgcacaa ccggtccaac tccgccttca cttcgctctc cgtcggctcg atcatcaagg
    49561 tgccggccac gggaaacgag accgtgggag cgtggaaccc gtaatccatg agacgcttgg
    49621 ccacgtccat cgcttccaca ccgctgctct ccttgagcgg gcgcagatcc aaaatgaact
    49681 catgtgcgac gaagccacgg gtgccggtgt ataaaacggg atagtacttt tccaatcgtt
    49741 tggccatata attggcgttc agaatggcca cttgcgtggc tttggtcaag ccctcccgtc
    49801 ccatcagggc gatatagacc caggaaatcg tgaggatgct tgggctcccg tacggcgcgg
    49861 cagcgatcgg accgatcgac tccggtccac ccagtttggt gaccgggtgt ccgggcagga
    49921 acggcaccaa atgacgggcg actccgatcg gtcccatccc gggcccgcca cctccgtgag
    49981 ggatgcagaa cgttttatga agattcagat gacagacgtc cgcccccagg tcggccggtc
    50041 gacagagccc gacctgggcg ttcatgttgg ccccgtccat ataaacctgc ccgccatgcg
    50101 tgtgcacgat ctggcacatg cggcgaatcc cttcctcaaa aacaccgtgg gtcgagggat
    50161 aggtgatcat caacgccgcg aggcggttgc gatgctgagt ggctttggcc tcaagatcgt
    50221 tcagatccac attgccacgc tcgtcacagg ccaccgggac caccgtcatc ccgcacatcg
    50281 aagcactcgc aggattggtc ccgtgcgccg acactggaat caagcaaacg tcgcgttgcg
    50341 tttcgccgcg atgccgatga tgcgcacgga tcaccatcaa ccctgcatac tccccctgcg
    50401 aaccagcatt gggttgcagc gaaatggcgg caaacccggt cagttcagcc aaccaggctt
    50461 cgagctgttg gaacagcgtc tggtatccct gcgcctgttc ggacggagca aacgggtgga
    50521 ggcgcccgaa ctccggccag gtcaccggca acatttccgc cgtcgcattc agtttcatcg
    50581 tgcaggatcc caagggaatc atggaatgca cgagcgacaa gtcgcgggac tgaagccggt
    50641 ggatgtagcg taaaagttca tgttcggcat ggtaccggtg aaacaccgga tgcgtgagat
    50701 aggggctcgt gcgcgcgagc ggtgcgggat aacgcacatc gaccgaggcg agaatggcct
    50761 gaaattcttc ggcagggatc tcatgaccga cgaagagcgc caacacctgc tggacttcct
    50821 caaccgtact ccattcatcc aacgaaagcc ccaggctctg atcgtcgtac tgacggagat
    50881 tgatcttctg ctgtcgcgcc cggttcagaa tggtgtccga ttgcgccggc gacagtggaa
    50941 cacgcaacgt gtcgaacacc ggctccaggc cgacggcaca accatgccgg cgcaaacctt
    51001 cagccagtac catcgtcagg ccgtgaattc gctctgcaat ccgccgcaac cctgcaggac
    51061 cgtgatagac cgcatacatc cccgccatga cggccaacag cacttgcgcc gtgcagatat
    51121 tgctggtggc cttctcgcgc cggatatgtt gttcccgggt ctgcagcgac aagcgatagg
    51181 ccacgcggcc cgtcacatct ttcgagacgc cgatgatccg gcccggcatc tgccggcgaa
    51241 actcctcctt ggtggcaaga aacgccgcat ggggcccacc gaatccaagc gggacaccga
    51301 accgttggct ggatccgacc gccacgtccg ctccgaattc tcccggtgaa cgcagcagcg
    51361 tgagcgccag caagtcggtc gccaccacga cgtaggcccc cgaggcatgc gcgcgcgtga
    51421 tgaactcgct gtagtcaccg acgtacccgt ccgtcgaggg gtattgaagc aacagcccga
    51481 agaactcgtc ccgggaaaga tccaacgact gaatcgcacc gatctgcagg atgatgccca
    51541 gcggttccgc acgggtctgc accaccgcaa tcgtttgcgg atggcaattt tcagagacga
    51601 agaacttctt gcgctcatgc ccggccgctc gagacatggc cgcacacatc gtcatcgcct
    51661 cggctgcggc agtcgcctca tcgaggagcg acgcattggc gagcggtaag ccggtcagat
    51721 cggcgaccat ggtctgaaaa ttgaccagtg cctcgagacg cccctgcgca atctcggctt
    51781 gatacggggt gtactgcgta taccatccgg gatcctccag aatgttccgc tggatcacca
    51841 acggcgtgat gcaatcgtag taccccatgc cgatcaaaga ccgccacacc tgattctgtc
    51901 cggccaagcc acgcaattcc gccaggacct gctgctcacc ccgcggggac ggcaccgtca
    51961 aggaggtctg caagcgaata tcggacggga ccgtcacctc gacgagggcc tccaacgacg
    52021 gcaggctcaa tgtggccaac atctcctgga tgtcggaatc cgtcgggccg atatggcggg
    52081 gcacaaacgt atcgttcggt tcaaggaaat tcggttgtgg catgggggtg aaatcctgct
    52141 aaaagttgac gctctcgtgc attcgaagcg gcgtgggcta acctatcatg caggaccgag
    52201 ttttccaagc gttcagcgcc catcgcccgt cgcttgtttt ccgaaattcc atagcgtaat
    52261 agtgacatcg gatgtccctc ccgggatgtc gatcgcacgg ctgaacggtg gccaggatac
    52321 gaagaccatg atcacacatg acatcttgat cgtcggagcg ggactcgccg gaatgcgggc
    52381 ggctattgcc gcaccgcctg acctcaatgt ggcactcctc tccaaggttc atccggtccg
    52441 cagccactcc gtggccgccc aaggcggaat taacgcggcc atcggggagc aggactcctg
    52501 ggaagcccat gcctatgaca cggccaaggg gggactttac ctgggcgatc aggatgcgat
    52561 cgaggccatg tgccgggaag ccccgcaaga cattttagag cttgaacgca tgggggtgat
    52621 cttcagccgc accccggaag ggcgaatcgc ccaacggccg ttcgggggag ccggatttcc
    52681 acgaacctgc tatgccgccg accgcaccgg gcacgcgctg ttgcacgcca tgtacgagca
    52741 gctgatgaag cgacggtgcc gggtgtacga agaatggtac gtcacggcgt tgctggtcga
    52801 acagggccgt tgttgcggag tggtggcctg ggatctggtc catggaggat tgcaggtctt
    52861 gcgtgcaaag gccgtcattc ttgccaccgg cgggagcggc cgcgtctttt cgaccagcac
    52921 gaatgccgtc attaacaccg gagacggcat ggccctggcc tatcgcgcgg gcctcccgct
    52981 ggaagacatg gagttcgtcc agttccatcc gaccaccttg aaagacactg gcattctgat
    53041 caccgagggc gcgcgcgggg aaggcggcta tttgctgaac acgctcggcg agcggttcat
    53101 gaaacgatat gcgccggagc agatggagct ggcgacccgc tccacggtgt cgctggccat
    53161 cggccaggag attcaagaag gacgaggcgt cgacggctgc gtcttactcg accttcgaca
    53221 cctcggccgc gcccgtattc tggagcggct accgcagatt cgagagctgg ccctggaatt
    53281 cgccgggctc gatccgatcg aaacacccat tcccattcga cccggcgccc attaccaaat
    53341 gggcggcgtg aaaaccaacg cttggggaga aaccgacctg ccgggactct tcgccgcagg
    53401 cgaatgcgcg tgcgtcagtg tgcacggagc caatcgcctg ggagggaatt ccctcctcga
    53461 aaccattgtc ttcgggcgcc gtgccggcac gagagccgcc gacgcgatcc gcgactatga
    53521 cctgcaaccg gttcccgaag aagtcctcaa aagtgaagaa cagcgcctga ataccttgct
    53581 gaccaatcca ggcctggagc gggcctggca gatacgggat gatctcggta agaccatgag
    53641 cctcaatcta ggcatcttcc gcaccaaaca gtccatgcaa gaggccctgg gggccattca
    53701 agccctcaag gcccgtgcaa accggatgtg cgttcaggac aaaggacgga tcttcaacac
    53761 cgacctgatc caggcgcttg aactccagtg cctactcgac attgcggaca ccatcgtcgt
    53821 gagcgctctc ggacgggaag aaagccgcgg ggcccactac agggccgatt tcccgactcg
    53881 aaacgacagt gcctggatcc ggcacaccgt cagccaccgt cgggaggacg gcccgcagct
    53941 gagctatgcc cccgtcacca ttacacgatt cccgcccgca taacgcctca atctccgcat
    54001 aagcactcct ctgtacggtg agtcatctct gagacggccc ggctcaggcc gtcggaccgg
    54061 ttgcgctcct accgttttgc gctatccttt tcaataatca ccgatcgctc cactcgcggg
    54121 accgtccaca atacgcccgg gcgaagggtc gttaggcacc atggcaaccg ttcacccagg
    54181 cacagcgata tctgcgtccg ccatcccttc gccgtcggat tcgacgcagg ccttcgtctg
    54241 ccccttctgc caaaccgtca cctcttgctt gcctgtcgcc ggcatccccc tgcaccaata
    54301 tgcctgtcac tcctgcaacg aaaatctgct gattctcaac ccggacgggc aaccagccga
    54361 tgaagacatg gtcgccccat cctcagccgg cgggtctgat gccaccgaca tgtcacggcg
    54421 tctcgtaggt ctcgctgtgg ctctgctggc ctgtgtcttg aactatgcac tgctggccgg
    54481 catggcgtcc tggatgttca atcagatcaa atcgacgcat gatccctatg acatcgatga
    54541 attgacgctc tccccgatta tcttgaaagc caacggttgg acagcgcttc acctcgcagc
    54601 ggcacgagga gatgtctctg aggtgacgtc gctgctggaa gacgggagcc cggtcgaccg
    54661 acacaataac aatggacgaa cggccttgtt cgaagcagca acgcgcgggc agacaccggt
    54721 ggtgacagtg ctgttgcaat atggagccaa ccccaacgcg aggatgaaac aaaacgtgac
    54781 cgcgctgctg acggcggctg agcacggaca tgccgacacc attgccgtgc tcctgtccaa
    54841 cggcgccgag atcaatgcgc tgtgcacttg cggtgattcg gcgctgcacc gggccgtacg
    54901 gaaagggcat ctggccgccg ctcaagtcct gctggaacag ggcatcgccg caaaccaaaa
    54961 gagccatgga gagaccgccc ttgagatcgc gcaacatgac gaggatcggg aattgatcga
    55021 gctgctgcgc gcgcacggag gccgagaatt ctcccaggcc aaggcccatc gtgcccaagg
    55081 cgtggccttc cagaagaagg gacaggtcga taaggcgctc ttcgcgtacg ccgaagccct
    55141 gaacctggac ccggatgatc cggaagccta tttcgaccgc ggcaccgcgc tgctcgaaaa
    55201 ggattccccc gatgaagcac tcatcgcctt tcacgccgcg atccgcctca attcaacctt
    55261 catcgaagcc tatagcgccg cggcctcggt acacgtcgcg cgccgacagt gggaccaggc
    55321 ctcagccttg tgggaccgat acctggcaca acggccacag gacggccgcg cccattttga
    55381 acgagcgatc gtcaaacggt cgaaggggga cagcaaaggt ttcttgcagg aactgcaaca
    55441 ggcctgcacc ttcgggtatc gggcggcctg ctgaaccatg accaccaccg acgcgcagag
    55501 cgagaccgcc gaccactccc caagcgtcac ctgcaatcgc tgcttcggtc gctatcggat
    55561 tcgcgacgca cgtcgattga cgggcagatc ccgaagcctc tgcccgactt gcggggcacg
    55621 attcgccgtc gtggtcgccg cgcctgctgt aaatcaacgg caaattgaag gacaggtccc
    55681 ttcgccggtg ctcctccatc ctccggcaac cgacgcccct gaatccaccc cctcaatgca
    55741 acgcgggtcc tttcacggag ccggcggcac cttgctgggc atgcagatcg tcaacgtctg
    55801 cctgaccatc gtcacccttg gcgcctacca cttttgggcc aaggcgaaga tccgccggta
    55861 cctgttcagt caaaccgcat tcgcgggaga ccggtttgcc tatcacggca ccggaaaaga
    55921 actttaccag gggttcctga aagcgacggt ggtcttcggc attccctatt tttcgttagg
    55981 cgccgcgcat agctttctgg ccctcccgca atcggtcgat ttcctattac aagcgatggc
    56041 tgcgctggta ctgttcctct atgtcccggt cgccatcgtc aatgcgcggc ggtatcgctg
    56101 cacgcgcacc tcctggcgag gcatccgatt ctcgtttcga ggacgaacgg tggacttcct
    56161 gaagctctat ttcaaagggt ggctcttcac cctcctgacc ctgggcacct actatcccta
    56221 tttccagacg cagcggcagg catttctcca ctcccacacc tatttcggca atcagcggtt
    56281 ccggtttacc ggacacggat cgggcctgat ggtgcccttt gccgtcacac tcttcaccac
    56341 ctatgcggta ctgtgcctgt gcggcctcgc cctggccctc cagttgacga acgccgggct
    56401 cacgctgctc ctgattccct tcgtgctcgg tccggtgtgg gtgtggctac tcgggcaaaa
    56461 acagaagtac tactgggatc acaccacgtt cggcgaggca cgattttcat ccagcatcac
    56521 ctggcaaaaa ctgttcggcc tctatctcgg caaccttgcc ctcttgctgc tcacgttggg
    56581 gtgggcctgg ccatgggtga ccgtccggaa cgcccgcttc ttcatcggca cgctttcgtt
    56641 gcagggtctc acggatctcg accgcgtcct gcaagacacc accgaaacat ccgtcaccgg
    56701 cgaaggcctg tcgaacctgc tcgacaccgg cttcgatatg gactagcccg catgcccacc
    56761 agccggagcg cccactatct cgacggtcgc acggccacgc gccatggcgt caccctcacg
    56821 gtcgcccccg cgaccctaca catcgtgatg ccagacggca gcaccaaaca atggccctat
    56881 gacgagatcc gccagacgca agggacttac cagggcgaac cggtccgcct ggaattcggg
    56941 cctgaaccgg ccgaagccat cgtgatttcc acagccgcgt tgctggatga cattcatgag
    57001 gcggcgccgg ctctcgcacg gcacttccac aacccggcca gacgacacgc gcgcatcggt
    57061 tggaccctct gtgccgcagt cggcgtcgtc ctcatggtcg tgggcctcta tcgatggggc
    57121 atccccggta tcgcctccgc cgcgacgccc tatatacccg tggcgtggga agaatccctg
    57181 ggccggcagg tcgtcgcaca ccttgcaccg gaaacacagc agtgtcgcga tccggagcga
    57241 ttgcgcaaac tcgaccacat cgtgcaaact ctggccgcca ctcatcctgc gtccccctat
    57301 cggatcaccc tttccgtcgt cgatgccccg accgtcaacg cattcgccgc gcccggcggc
    57361 cggctggtga ttctccgcgg gctgctggaa cagacggcca gccccgaaca actggccgga
    57421 gtgctcgccc acgaactcca acatgtctat caacgccata cgaccaaagc cattcttgaa
    57481 cagacagccg ccacgttgct gctgaccgcc gtctccggcg acatgtccgg cgggctcgcc
    57541 tggggcctcg aaggcgcgcg cacgatgggc tcattgcact acacccgcaa ccatgaaacg
    57601 gaagccgata tcgaaggact tcgtatgatg caggccgccc gcctcgatcc cgccgccatg
    57661 attgccttct acggaaccat ggagagaggg gcgcaggacc atgccgggcc gcctgatttt
    57721 ctctccaccc atccggacat gggggagcgg ctagccacac ttatcgcgct ggccggcccg
    57781 tccccgtccg acgcacaacg gctcctcccc ggagaagact ggaaagatat ccgtacactc
    57841 tgccgcctac aggcaggcgg gcgttccgcc tcagcctccc ccgagttgtc ttagccccgc
    57901 gattccgctg ctcacagatc cgatggcctc tggtataatc caccgactta tgggccccat
    57961 gtatgcgcgt cctcgtcatt gaagatgaaa ccaaggtggg ctccttcatc aagcgagcgc
    58021 ttgaggagga gagttacgcc gtcgacctct gcgaggacgg cgcgcaaggc ctcgacatgg
    58081 ccttgagcgg cagttacgac ctcatcatga tcgatctcat gctgccgagc ctgccagggc
    58141 tggaagtgct cacgcgcctc cgcaaggaaa agattcagac tcccgtgctg atcctgacgg
    58201 cgcaatcgaa agtggaccag cgggtcaaag gattggacgc cggggcggac gactacctca
    58261 cgaagccctt cgccatcgat gaattgctcg cgcgtgtccg ggctctgtta cgacgcggca
    58321 cggccgaatc ctccggcaca ctccagatcg acgatctcct gctcaatccg gccacacgcg
    58381 aagtcacacg cggcggacaa cgcatcgagc tgacggtgaa ggaatatgcg ctgctggaat
    58441 atttcatgcg ccatgccgga cgggtgctga ctcgtcccat gatttccgac catgtctgga
    58501 atcaggactt cgacacgttc accaacgtga tcgacgtcta tgtgaactac ttgcggaata
    58561 agattgatcg cggacgcgct cgaaagttga tccatacgat tcgagggagt gggtacatgc
    58621 tcaaggtgga ctgatgccgc ttcgagtccg gctcacactc tggtacggaa cggccctggc
    58681 cctcatcctg ctgacctttt ccgtggtgct ctatacgatc acagcgcgcg gcctccggga
    58741 tcaagtcgat gagtcgctgc aagaaaccgc atcggcagcc gtgcgttcac tggagaagcg
    58801 cggatttctc cccttgatcg acgaagacgc gctgttgagc caattccccg aattgacccg
    58861 catcgacaaa ttcttccaga tcttcagtcc taccggcacc atcaccatcc gctctcccaa
    58921 catccggcaa cacgacgtgc cattgagccg ccacgcgctg gaagccgcct ttacgggcag
    58981 caccgtcttc gaatcggcca ggtatcccaa cgaacctcct ctccgcctgg tctcagttcc
    59041 catcatctac cgcggctccc tgctctatat cgtgcaagtc ggcaccaccc tggaaggtgt
    59101 cgaggaaacc cttcgccggt tcttactcgt cctcgtggtc atggcgccga tcgctctggt
    59161 cgtctcattg gtgggaggat ggtttctggc cggacgcgcc ctccggcccg tcgattccat
    59221 taccatcgca gcgcaacgca ttgccgcagg cgacctgaca cagcgcctga cctccgaacg
    59281 ctccacggat gaaatcggac gactcaccga caccttcaac aacatgatcg cgcgcctgga
    59341 aacctccttc gcgcaaatcc ggcaattcag cagcgatgcc tcgcacgaac tccggacccc
    59401 gctgaccgtc atgaagggcg aaagcgagct ggtcctccgt cgtcctcgtc ccgtcgaaga
    59461 ctatatcgcc gtcctggaaa gcaatctgga ggaaatcgac cggatgtcgc gcatcgtcga
    59521 ggaactcctc ttcctctcgc gggcggatct gggccaggtc aaaacagaat gtgagccggt
    59581 tcgcctggaa gccttggtgg aagatatcca gcgtcaagcc tgcctgctcg gtcaagagcg
    59641 tggggtagac gtgatcatgg gcaccattca accggcaacc gtccgtggcg acgaactccg
    59701 cctgcgggaa ctcattctga acatcgtcga caatgccgta aaatactcct acccgcaggg
    59761 cacggtggat atcgatttgc tggtggaagg ccccacggcg cgcctttcag tgcgggacca
    59821 cgggatcggc atcccccagg acgcgcacaa acaaattttt gaccggttct tccgcaccga
    59881 cgacgcacgc tcccacacga aaaaggggac cgggctcggc ctggccatct gcgcctggat
    59941 tgccgaggcg caccacggac acatcgaagt ccagagcgaa gtcggcaaag ggtcgacctt
    60001 caccatcgtg ctgcccctcg ctccgctgac agcttaacaa cttttaatcc ctcgctaatt
    60061 ggtttctcat ccacggttgc taccttccag agtgtctgct gcggggtctc tcccgcgcac
    60121 acagatcacc gacttcagga ggaggagccc catgaaacga cctacgcagt ccgtcagtgc
    60181 cattaccggc atcgccgccc tgagcgctgc cttgatatgg agttatctcc cgctcaccac
    60241 ctcacacgcc tcgaatccct caccggccgc agaggagcgc ccggccacga tgacgggtac
    60301 cctgcccgca gccggattcg cagatgtggc caagacggtt acacccgccg tcgtgaacat
    60361 caccacgagc ggcgcagaag aagtgtctga ttcctcacgc ccgcgtggcc gcatggagga
    60421 ttttttcgga tcgccgttcg gtccacggcg attcggacca ccgatggaac cccgggaacg
    60481 tcgtggcgga gggcagggat cgggcgtgat cgtcaccccg gacgggtatg tgctgaccaa
    60541 caaccacgtg atcgcaggag ccaaaaccgt caccgtcaca cttccggaca agcgggagtt
    60601 caaggggcgc atcatcggca gtgaccccaa aagcgatatt gccgtggtca agatcgacgg
    60661 cacccagctt ccgacagtca cgtggggcga ctccagccgc ctccaggtcg gtgaatatgt
    60721 cctggccgtc gggaacccat ttggtttgaa ctcgacagtc accttgggca ttgtcagcgc
    60781 cctcggccgc ggacacatgg gaattacgca gtatgaagat ttcatccaga cggatgccgc
    60841 gatcaatcca ggcaacagcg gcggcgcact ggtgaacacc aggggtgagt tgatcggcat
    60901 caacaccgcg atcttttctc agaccggcgg ctaccagggc gtcggcttcg ccgtccccac
    60961 gggcatgagc aaaccgatct atgagagcct ggtgaaaacc ggcaaagtcg tacgcgggta
    61021 tctgggtgtc gggatacagg atctgaatca ggagttagcc aagtcgttcg acgtcaaagg
    61081 aagcaatggc gccatcatca ccgatgtaaa ggaagagggg cctgcagaca aggccggcct
    61141 gaagcaggga gacgtgatcc tgtcctttca gggaactccg atcgaagacg ccgtcaccct
    61201 tcagcgcgcg gtcacacgaa gttcggtcgg cagcaaggct acggtaaagg tgatgcggga
    61261 tggccgtgaa aaggacctca ccgtgaccat cgctgaactg ccggacaacc cgcaggtcgc
    61321 caaggctgag gccggtccat cggaacagcc gctggccgga ctcgccgtgc aggagttgga
    61381 ccgggagact gcccaggaac tcgggatcaa aggaaaaatt caaggcgtcg tcgtcacgac
    61441 cgtcgatcca gagagcgacg cggagcgtgc cggattgatg ccgggcgacg tgatccggga
    61501 gatcaaccgc aaaccggtca cctcgatgaa ggatttcgat cgggccgcat cagacctcaa
    61561 gaaggggcaa acggtgctgg tgctggtcaa tcggagaggc gcgtccctgt acctcagcgc
    61621 caagatctaa gagcggccgt atagagcagg caggctactg gtgagcgcag gcttatcggt
    61681 agcctgcccc tgattttttg ggcatgagga gagtcggaac agcgggcgca gcagtcggcg
    61741 cgtcggcccg cccaacgggc tgggcaatta gatcatatcc ggccacatcg gtaacggtga
    61801 ccgaaacaaa ctcacccggc ttcgccgcgc tgcgatcaca gaagaccacc ccgtcaatct
    61861 caggcgccaa cccttcgtgt ctgcattcaa gcagacggtc cgattcttca gagatcccat
    61921 ccaccaggac ctccatcgtg cgacccaggt agtcacgatt cttggcttct gaaatcgact
    61981 cctgcagagc caggagttca ttgcgccgtt cttccatcac cgcctggtcg actttccggt
    62041 cgagatcgac ggccgacgtg ccttcttcat cagaatagag aaacaccgcc acgcgatcga
    62101 attccatatc gaggatgaat tgcttgagct cctcgaacat ggcgtcggtc tcaccgggaa
    62161 atccgacgat aaacgccgtc ctgaaaaaga cgccgggaat cttggctcga atacgttcga
    62221 ccagcttggt gacgtgtttg cggtccccca accggtgcat ccgcttcagc atgccgtcgc
    62281 tgatatgttg caacggcatg tccaggtact tggtgatacg cgcctctccg gcatagagat
    62341 ccagcaactc atccgtcacc tgttgcgggt acagatagaa gggccggatc cagcgcacat
    62401 ccttcaccgt caccagtgag cgaagcagcg ccgtgagccc ctgtttgagt ccgagatcca
    62461 ctccgtaatt gatcgtgtcc tgggaaatca ggttgagttc cttcacccct tcttgaacca
    62521 accgtccggc ctctgccaca atcgattcaa tcgggcggct gcgctgcttg ccccgcatga
    62581 tgggaatcgc acagaacgcg cagttccggt tacagccttc ggcaattttc aaataggcgc
    62641 tgtggggagt tccgaggcga atgcgtggag tctcggcatc gtataaataa ggcggttgcc
    62701 caatccagag tcgctgctga cgagctttcg gcgccagcaa actgcgacag atctccgcga
    62761 tgcgcccgaa ctcgcccgtt cccacgacgc cgtccagctc aggcaattct ttcaacagtt
    62821 cgccctggta ccgctgcgcc agacacccgg cggcgatcag gacgcggcag gatccggact
    62881 tcttcaaccg tccatgttcg atgatgctgt tgatcgactc ctgcttcgcc tcctcgatga
    62941 agccacaggt attgatgata acgacttccg cggcgcgcgc gtcgcccgtg agttgaaagc
    63001 ctccggcgac caatgtgccg agcatgactt cggagtccac ctggtttttt gaacaaccga
    63061 ggttcacgaa gccgatcgtg ggtttgttgc gtttcgcccg tgatggggta atcaatgacg
    63121 aggccataat ttggcagtct acgagacgcc ggaaaactct gtcaaacgag ctctcttgct
    63181 attccgtctg cagctatcct aaattgagcg catgatccgg acatttcagg gcatcacccc
    63241 gaccgtcccg cagtcctgct ttattgaaga gaccgccgtg gtcatcggcg acgtcgtcat
    63301 gggcgaagag tgcagcgcct ggtttcatgc cgtcattcgc ggcgatgtga actacattcg
    63361 catcgggcac cgcacgaacg tgcaggacct ctgcatgttg catgtcacac acgacacgca
    63421 tccgctcatc atcggcgacg acgtcaccat cgggcacaac gtggtcctgc atggctgcac
    63481 cattcacaat cgtgtgctgg tcggaatggg cgccatcatc atggacggag cggtcatcgg
    63541 ggaagactcg gtggtcggcg ccggagcatt gatcaccgaa gacaccgtcg ttccgcccaa
    63601 gagtctcatc ctgggctcac cggccaaagt aaaacgcccc gtcaccgagc aggaactcgc
    63661 ctggatccgt gagtcagccc aaaactatat ccgctattcc cgccaatacc tctcgggccc
    63721 ggataagcct cgccccggat tctgggtcta gtaccatcca cgcacagact cgccttcgac
    63781 aactcgcttc ttacccgatc tgcataaaac agatggggtc accaaccgtc ggcgaggcat
    63841 acgtgcgctg gtcgatccga tagcggactc cccgccgatg gcaccactca gcaacccaca
    63901 ccgcctcctc caccgtcgtc gtcaccaatc ccgggatgat caatcgtttg aggcaccggt
    63961 ccaccaaggt agcgactatg cgttgctgag gcaaaaacct tttcgcgtcg aacgtcaccg
    64021 gattcgctcg accgtaggac aatgcggaca gttccggaaa ccgctccgga tcgttgagta
    64081 cgctgacaag ccagaggtga tccacccgat ctttcacgga cccggcatcc tgatgacgga
    64141 acctgatcgg gaacccaaca caggcacgat tgagcacctg cacttcctgc ttgcgtagat
    64201 tgcaggccag gacctgtcgc ccgagatcca ggctttccat cagaaggagc ggaatttcag
    64261 ccacccccgc gcccacatat aaactggtgc cacccggctg aagccgcggc aggagcgcct
    64321 tcgccagttt cagaccaagc cgctgacaaa gcccgcgctt cgccgcccag aattcatctc
    64381 cgccttcgtc gcaatagatc ggacccagtc gcccgtagtc gaggcgcgcg aagatgtcct
    64441 tcatcgcccg ccgcgcgacc gtccccacgt tcatcagttt acgggccatg ctgctccatc
    64501 tcgaccaaac ctgtggccgt tctcccggcg ccaacggcca ccgggcgcat acaattgaga
    64561 gtcaccttat ctcgcctgtc tcttgagtaa cgagaatgtg ggaggcctga gattcgccgt
    64621 gactggaaca cgaccggccg gcaaggctcc atggcagcac cgcagagccc ttccacgacg
    64681 actcgcgtct gcgcatcgtt acggcgccgc gtcaggaacg actacgcgac gatctctgtg
    64741 ccgataccct tgtgagtgaa gatttccaac agaatggcat ggggcgtacg accgtcgatg
    64801 atgtgcgcct tccccacacc cccggccaat gcatccaggc aagcgtgaac tttgggtaac
    64861 atgccctcgc tgattgtccc gcgcttgacc atccgctgca catccttgcg ggagaccgtc
    64921 ggcaggtggc ggccattggc atcgcggatg cctttcacat cggtcaacat cacgagtttc
    64981 tccgccttca acgctgcggc aatcgcgccc gccacgaggt cggcgttgat gttgtacgta
    65041 ttgccttccc gatccgttcc gatcggcgcg ataacgggaa tgtaattgtc ttcctggagc
    65101 ttcaagagca gcgtgggatc gatcgatttc acatcgccca caaacccgaa atcggcatcc
    65161 gaatcgtccg aatcgaggtc cttttcaatg ctttccgccc aggccttcgc cgtcagcggc
    65221 ttggtcaaca tcaacccgcc gtccttgccg ctgaggccga ccgcctgtcc gccgtgtcga
    65281 ttcaagagat cgacgatttc catattgatc cggccggcca ataccatctc cacaatttcc
    65341 atcgtggccg cgtcggtcac tcgcaccccg tgccgaaact tcgcgaccat tcccagtcga
    65401 tccagcatct gatcgatttg cggccccccg ccatgcacga tcacggggtt gagcccgaca
    65461 tacttcagca acaccacatt ctgggcaaat cgctctttca ggggcgcatc ggtcatcgca
    65521 tgccccccgt acttgatgac gaccgtcttg cccttgaagg tacggatgta gggcagggcc
    65581 tcgatcagca cgtccgcttt cttaatcaat ttgttcatgc gctccccccg tccagcctgc
    65641 cgcttacaga atatagcggc tcaagtcttg atccttcaca atattctgca agcgctcccg
    65701 cacatacccg gcgtccacga cgatggtctt ttcgttcatc tcggaccctt cgaacgacac
    65761 ctgttccagc aaccgctcca tgatcgtaaa cagccgccgg gcgccgatgt tttccgtccg
    65821 ctcgttgacc tgtaccgccg tcgccgcaat ctccgccaat ccgtcatccg taaattcgac
    65881 ggtgagccct tctgtcgcca tcaacgcctg atactgacgc accagcgcac cgcgcggttc
    65941 ggtcaggata cgcacgaagt cgtctttggt gagcggcgcc aactcgacac ggatagggaa
    66001 ccggccctgc agttcgggaa tcaaatccga cggcttggcg acatgaaacg cgcccgcggc
    66061 gatgaagaga atatggtcgg tctgcacggc gccatgtttc gtgctgaccg tggaaccttc
    66121 cacaatcggc agtaagtcgc gctgcacccc ttcgcgggac acatccggcc ccatcgcgcg
    66181 ctcacgcccg gcgatcttgt cgatctcatc caaaaagaca atgccggtct gctcgacctt
    66241 cgtgatggcc tcccgaacga cttcgtccat gtcgatcagc ttttgcgctt cttcctgggt
    66301 gagatgcttc agcgcatcgg gcactttcat catccgtttc tttttcttcc cttgaaacat
    66361 gccgcccagc atgtcgcgca agttcccttc aagatcctcc atgccgccgg cattggaaat
    66421 cacccccagc ggcaacgctc gttccttgac ctctacttcc acagatcgct gatcgagttt
    66481 gccctcgcgc agttgcagac gaagcttgga tcgcgtggca tccggcgatt cggtcggctc
    66541 cgaagcatcg gcacgcgccc cggaaggttc aaatccaggg gaagtcggac gggacggtgc
    66601 accaggcaac aggagatcga ggagtcgctc ttctccaagg cgcgacgcct tcccctgcac
    66661 ctcttccaag tgtttggtct tcaccaaact gatggccagc tcggtgagat cacgaataat
    66721 cgattcgaca tcccggccga catacccgac ttcggtaaat ttggaggcct ccaccttgat
    66781 gaagggcgct tcggccaatt tcgccaggcg tctggcaatt tcggttttcc ccacgccggt
    66841 cggtccgatc atgatgatgt ttttcggcat gacctcatcg cgtagttcag gagccagctg
    66901 ctgacggcgc cagcggttac ggagcgcaat cgcgaccatg cgcttggcat cgtgctggcc
    66961 gatcacatag cggtccagcg cttcgacgat ctgccggggc gtcagactat tcacattgag
    67021 aggacgggac gtcgattcgg tcgtcatggc aggcagatcc taacgcgaga gcgattcgat
    67081 cacaatctgt tgattggtat aaatgtcgat cccgccggca atcatcaggg attcccgcac
    67141 gacctgttcg gcaacgagtt cggaatgccc cagtaatgcg cgagcggcgg ccagtgcata
    67201 gggccctccc gatccgatcg ccaggatgcc gtcttccggc tcaacgacat ctccggtccc
    67261 ggatatgatg aaggagtgat cgagatcggc aacggccagc agcgcctcca agcgccgtaa
    67321 cacgcgatcg gtccgccagt ccttcgccag ttccacggcg gctctagtga gatttccgcg
    67381 atactcggcc aatttggcct caaacttttc aaacagggtg aaggcatcgg ccgtggcgcc
    67441 ggcgaacccc gccagcaccg tatcgccgtg catacggcgc attttcctgg cattgtgctt
    67501 catgaccgta gtgccgaccg tgacctgtcc atcacaaccc atcgtcacct gactgtctcg
    67561 cctcacacac agcaccgtcg tggatcgaat aatcatcgag accccttctg cttgatgact
    67621 tcgctcgcct ccgtggtacg cacggcacgc gggtgagtgc gatcgtataa cgcgagcagt
    67681 tggtccgtcg caagatgtgt atatttttgt gtcgtactga gcgaggcatg tcccagcatt
    67741 tcctgaatgg cgcgcaggtc ggcgccttca tccagcaaat gcgtggcaaa cgaatggcgc
    67801 aaggtgtggg gatggaccgc gcctccggtc agttgccgcg aatatttggc cacaatgcgc
    67861 gcaatggttc tggtggtcaa tcggcccccg cggctattgc gaaagacggc cacggcgtcc
    67921 gacgatcgtc ctgaggacgg tcccgggacc gtctggacct gcgcgtggta ggcatcgatc
    67981 gcctccagcg caacgctgcc cagcggaatg acccgttctt tccgcccctt cccgcgcacg
    68041 cgcaccagtc cttcgctccg gctgatatcg tcgcaattca tgccgaccaa ctcactgacg
    68101 cgagctccgg tggaatacaa cgtttcgaga atcgcgcgat cgcgcaaacc ttcccgttcc
    68161 gttccaccgg gaaactccat caacgcgccg gcatcgtctt tcgtgaggac ctggggaagg
    68221 tgtttgggaa gcttcggggc gcgcacatcc tctgcagggt tcacttccac ttggccgaca
    68281 cgcaccagat accggaagaa actgcgcagg caggccagtt ttctcgccag tgacgtcttt
    68341 ttttcacccg ttcgatcacg agcggcgaga aactcacgaa tcaaagcagg cgtaaccgat
    68401 tcgggcacca gcggcccacc aggcttttgc acaccaagcg cgaaggcctg aaactgagcc
    68461 aggtcagacg cataggcacg aatggtttgg ggagacgcgt tctgttggac agccaggacg
    68521 tctaagaaag tccggattgc gcgatccatg cgtcaaaatc ctcaacggct cgttgctgga
    68581 tgagacgccg cttctgatcc ttgtcgcgcg tcttgacggt gacaggcggg aacagcccga
    68641 agttcgtatt catcggctga aaatgggccg gatcgctctt ggtaatatgc gcaattaaac
    68701 agccgtgggc gctgttcggc ggcggagtca ccaacggctg gtccgccagg cctcgcgccg
    68761 cattgatgcc tgcaatccct cccatcgcgg ccgactcggt gtacccttcg accccgacca
    68821 attgtccggc aaaaaatacg gtgccacggg acttgagctg gagcgtctcg cgcagcaatt
    68881 gcggcgagtt gatgaaggtg ttgcgatgga gactgccgag ccgtagaaac tcggcattct
    68941 cgagcccagg aatgagacga aacacccgtc gctgctccgg gtaggtcagt ttggtctgaa
    69001 agccgaccat gttgtaacag gtcccgtgcg cgttctcggt acgaagctgc accacggcgt
    69061 aggggcggac cccggtcttc ggattctcca gcccgaccgg cttgagtggt ccgaactgca
    69121 tggtctgccg gccccgctcg gccatgactt caatcggaat acaactttca aaataggcaa
    69181 ttttctcgaa ctctttcggc tggaccttct cggccgtcat catcgcgtcg tacaacgcat
    69241 tgtacgcagc ttcatccagc gggcaattga ggtaatcagc cccccccttg ccgtaccgtg
    69301 acgcacgata gacgatgtcc atgttgatcg actccgcatc gataatgggc gaaatcgcgt
    69361 caaagaaata gagatgccgc tcgtgcgtca attcgctgat ggctttcgat aacttttccg
    69421 acgtgagcgg accggtcgcg atgatgcaga cggcgtcctg cgggatctcc gtgacttcct
    69481 cacgcatgat ccggatgttc ggatgcccct ccagcgcctg cgtgatcacc tgggcgaact
    69541 gctcgcgatc gaccgctaac gccgagccgg ccggcacacg cgcctcctcc gccgcacgaa
    69601 tcactagcga gttcaggcga cgcatctccg tcttcagaat gccgggcgca ttgagcggat
    69661 cggcggaccc aagggagttc gaacacacga gctctgccag atcacctgtc ttatgtgctt
    69721 tggtcatctc tttggggcgc atttcgtaga gcgtcacctt ggcgccacga ttcgctgcct
    69781 gccaggcggc ttctgtgccg gccagaccac ccccgataat cacgatgtct tcacgcatga
    69841 atgggaaacc tttgaaaaga tggaaggagg cccattgtag gaatcgcctt ttctcgatgt
    69901 caagaaacgg agggcttagt ccttgtagcc ctcgctccat gacgccatct gaagccgatc
    69961 aattaaaggc gagctggaaa aggccctctg agtttgttgc tcccttttgc cagaccatgc
    70021 tagactctcg ccgcgtgggc gattagctca gggggagagc gcttccttca cacggaagag
    70081 gccacaggtt cgatacctgt atcgcccacc aatttttcaa cgacttccaa gacagcactc
    70141 tccgacacac gcagcactgt ggtcattttt gtgtcacggt gtgaccgtcg gtccaaaatt
    70201 tccactccat ctcgcaggct ttccgggtaa tgatgcgcat agcgttgcgt catcatcggc
    70261 gacttgtgcc cgagtagccg ttgcaccttg tagagatcga ctcccgactg gaccagccgc
    70321 gttgcgaagg tgtgacgaag gtcatggaaa tggcagttca cgatgcccgc ctttgccatc
    70381 gcaggccgta acgcccgtcg cagatggttg ggatctaacc gtgtaccggc gagactggga
    70441 aagactagtg cggttttgat gtgccgcacc ttggcctttt ccgtcaggag tgccatcaga
    70501 gtctgattca atggcacggt ccgccgctcc ccgtttttcg actcaaacac cgtcgcggtt
    70561 cttcggaaca gatccactcc ggcccaggtc agtgacagga tctcgcccaa ccgcatccca
    70621 ctatggagtg cgaacaccac gagctcgcgc aaccacgacg gacaggcatc gagcaatcgc
    70681 gcttcctcgt cttccatcag ccaccggtct cgtttcgtcg ctcccttttc gagagacacg
    70741 cggctgacgg gattgtctcg gcaccattcc cattcccgct tcgccaggtt gaaggccttt
    70801 ctcaagcagg tgagctctcg attgatcgag gcaggtttca ccccggcagc ataccgtcgg
    70861 cttttatact ccacgatcag cttgggggta atctcgccga gcgtccggtc tccgaagaag
    70921 gcacggaagc gcttgacgaa ggcctgcccg gtttgtcggc tggccaattt cacgaggtgc
    70981 tctcgctcaa agcgatccat caattccttg aaggttcggg atttttcttc agcccgatcg
    71041 aaatactgcc cctcgatcaa ttgcaccttc accttcgcca tgatggcgtc agccaaggct
    71101 cgattcgatg accccgtcga ccggcgaatg cgctgcccct gaaacacaat attcatccac
    71161 caaatattcc ctcgcttaac gagccccatc gttactctcc tttccaagag ggctcgctgt
    71221 tggtctggtt tccccgtggc gaggagtata gacggcgcgt ttggccgctt caatgaggtg
    71281 gtctacgtca ccagctttat gccgaggcac acgagggggg aggttcgaag acgtggaaga
    71341 aaagccattt agccacgcct ggatggcctc aggttcgaag cggacgaggc catgaatccg
    71401 acggcaagga atcttgttct tggaaaccca gagatagagt gtggattgtt tgatattgag
    71461 ccaaaccgag agctctttga cagtgagcat gtgcagatcc accgggggag tctgacgaca
    71521 cccccagtct ccctaacatg ggctgcccgc gtgaccgccg gcagaccggt gaaaccgagt
    71581 gggtggttgc ttgagcaaat tgcggtgtcg gtcgttccac cgagcaatgc ccctgacaat
    71641 ttcgttcagt agccattctt cccctccagg ggtggcgcaa atcaccgcca acatcggcgt
    71701 cagggtgtca ctcacccatc gtttcacgtt ctgcagggtc tgcacctgct gctcctgggc
    71761 caatcgcccc ttctgaaatc cctccgtcag gagggcgtac cactccagca ctggagcccg
    71821 gtagcgttct tcatcctcgg catcctttga agtctgccgg aaatccacat aggaccggag
    71881 caacccaatg actaactcct tccagtcggt ttcgtctaac gttgccagtg cccttgcgca
    71941 ctgttcggcg cgatcctttt tgagctctaa ttcccaccga gtcccgtact ccggccagtt
    72001 ctcttgtcct ttgctctgaa gttccagccg tttgtcgtaa atgcgcaata aggtctgact
    72061 ctgaggactc ccgaaataca tcgtctcacc cgtcgtcgct ccggtcccat gcgtcaggtt
    72121 tgagacgata tgccggactt gagccgccct cgtgacgcac tggcctgcag caaccgcctc
    72181 tcgaatcgtc ctgaccggga ccgttcccgc ccggtcatcc agggcgcaat caatgcgcgt
    72241 gacgtgtcct tgctgcttat gcacccactg gaggagggtt cggatttgat ccagtgtcag
    72301 ggcggacgcc aggccgcccg acagatccac atggatttca ttcggacgcc gaggggcatt
    72361 tgtgcccaat ttaccgaccc cgcgcaggcc gtctactcgc atccaggaca aggggtagcc
    72421 tcggaagccg cctttggcct tactccagtc tccgccaagc accttcatcg tctcttgggg
    72481 attgctcgcc aggaccgtaa acgccagcca atcaatggtg agcgtgaagc tcgaatccat
    72541 agaatctatc gaactcctgt acggagcgct gtgggtagcg cccccgtgtt accaagacgg
    72601 gggccattcc gctccgcccc gcagcctctc ggcatgcggc gcggaccggc tttgtctccc
    72661 ggcgaaggac gacggtgttg ttctgaacag gactccattc gtggtgtcat tgcatccttg
    72721 tgaccgttcc gttgcacgct ccgaccttat gcaaacgcca ccacgcagta aagaaccctc
    72781 taggggacag gaggggtcac gaggggacaa atagcggaca aattatcgga acacggaggc
    72841 cagaagggaa gtaggcataa gcatgcttat gtgcgctttc gtgcgctggg agcggcaatt
    72901 gtgcgcatcg ttcgaaggcg ggacagggag cgaacgcaaa tcagctcgtt tttgactggt
    72961 gcgcgaaaaa aaagtttgga aggcattctc cacgttctgg gatgcgatca gcgcgaggtg
    73021 gatcagtggc gccgacgact agaaggcagg gagcacttat ctaaacggaa aactgtggag
    73081 cctcagagat ctctgaggct tcgctctagg cttagcagcc cctatgaaaa ctgctttgta
    73141 tcgactctca aggttcacgc aacgcacttc aagggtctta ctgaattcgt ttgccaatcc
    73201 tttcccatcg tacccattcg ctccatgcgc cttgtttatt cactgaccaa taaataagcc
    73261 tagccctacc tgtgattttt gattcatgaa catagcccca aaatcgacta tcgaggctgg
    73321 catcacggtt gtctccgagt acgaagtaga actgttccgg cacgattacc ggaccgaagt
    73381 tatctcgtgg gtgctcatga cccgggacca cgcgaggatc aagtcgctgc gtgtaggagc
    73441 catcatcaag gggcacccca tttacaaaaa cgatcttatt acgaatttca atcctatctc
    73501 caggcagacc gatcacccgt ttcaagaagc tctttccttc atccttcggg tagaggaagg
    73561 tgatgatctc tcctctgcgt ggctcaaatt tgatatggga gatgagagtg tccacataga
    73621 tatagtcgtc agccagcacc gtcggagcca tagattctgc tgggattttg tgcgctttaa
    73681 ctatgcctct cataagatct ggcataaacc aagcgagaca tcctcctatc acaaataccg
    73741 caagagagaa ctgcttgctg ggtaaagatt caggcagacc agtttgctgc ctgcgtcgag
    73801 cgacacgcac cgccgaacgg acaagaaaaa tgtaccctat tagcggaacc aatattccag
    73861 cgagatttaa tcctgtagga agaaggggtg atctggcaat gagtacagat gcaagagcca
    73921 gcaactgaaa agtcacaaac caatacacac cttgcttggt gcggccgagg tacacatgcc
    73981 caaacccggg caatagaatg tttagcaaac cagcgatcca aggttttcgt tccgcttgag
    74041 gtctggcagg tggcatagtt tccgatggca tcctatgtgg gtgttttgag gacaccagaa
    74101 tatggcaagt ctaacaaaaa agatctctcc cgttcgagaa agtgttatct aacaaagagg
    74161 agtaaacgaa gcggattgcc ggaaacatgc accgcaaaga caaggaaacg aggtcgggag
    74221 ttcttgtagc gcgagctgcc gcccactcgc gcaccgggta gttactctca cctcttcgca
    74281 ctatggcgca aggcacggat cgactagcgg tgcgcgagca ggcgggcaaa ctctgcggct
    74341 gaaatgaccg gcacaccgct gtagtgtgag gcctcgaaat ccttatcccc ggtgacgaag
    74401 tattgaggtg gggggatggt cgccatgaga tcgaggaatg gtcgatcatg cgcatcacgc
    74461 agaggggtat tggtgggatg gggatcgacg atctgcactt cggcttgaat tgtttccaga
    74521 aagctcgtga tattcacccc ggctcgggta aacgcgcgtt ggagtttggg gcgacgcaga
    74581 acggcttcca gttccgcgaa ggtatcggag ctcatgacgg ggatgaggcg gcgctgtaat
    74641 acagcctgaa tgatccgggc agggggccct gcggcggaca acaggccgga cacgaaaata
    74701 ttggtatcaa gaacgacgcg cacgctcgcg ccgaaccgct gcaacctcag cggcaatctg
    74761 ttcgggggtg acgcccagtt cgcgggtggc gcggcggagc ttgctgacca tacggaccag
    74821 cttcttgcga ctcgccgctc tggccgggga ctccaagatc aggatatcgg aatcgcgatg
    74881 cgccacgacg gcttttcccc accccttcat ccacgaggag ggaatcacca cgccttcttt
    74941 ggtgtacttc acggctttca tcggcagcac atccagtgta tccgcaggca gtataccatg
    75001 gtgctgcttc tctgtagtca gcggtcggcg attttgcgag ccgctcgacc gtcgttcatt
    75061 gctagactgt tcggactgcc ccatttttgg accatccccg ctgattaacg atcctgtctt
    75121 ccgaggccag ttcactgtgg tcatttttgt gtcacaaatt atccaaaacc ttaaaatctc
    75181 ctgtacacag tagacgcgat agaaagctct taaccacatg acatgtaacg gttttgtggg
    75241 gaaaccgttg caacagcgag caattcaaaa atctagcttt tcaccttcac acggaagagg
    75301 ccactggttc gataccagta tcgcccacca ttcatatccc gaccgtccca acaccactac
    75361 cgccctgcaa ccgctcaatt tatctcgaca tcctacggca gccagctgcc cgcgccctca
    75421 actggacaga atcaagcgcc tgagctagac ttttctcaca aggaacagac ttcgttttta
    75481 cgaatgttcc gacagaagaa agagaggcat ggcatgcgcg ggactgtagc aacgatcata
    75541 ttgggactgg ttctgaccac gggctgctcg accaccaatc aggtggcgac acaagaccag
    75601 aactataatg cgccgctcag tatttatagt gcgatggaca gcacctcact ggtctattcc
    75661 gatcccatgg ccgcctcaca agccaacgac cacccttatc gatgggctgc gttcatcctc
    75721 catccagtcg gtcacctcgt tgactacacc gtaaaccgcc ccctctatgc cattgccaaa
    75781 cacgcaccct acctcttcgg ttatacatcc gaagattcca tgatcgactc gcagcgacgg
    75841 taaccctgct cgagaccttg ccggccggga cctctcccgg cggcgaggtg agcgttcctt
    75901 gcccgcctat tgccagtcgc atctcccttg ccgttcaggt atgctcgcca cctcgatcgt
    75961 gcgccacgtt gaaccggggc atcgaatccg gccatgtatc gtcaactctc actgctgccg
    76021 ttcttgttcg ctgcctgcgc cctctgggca ggatgcgaaa ctccgccacc ggctcggcct
    76081 ccgcttcctc cctccgagag tgatcttgac ctcctgggac ccacaaaact ctgcgattcc
    76141 cgccagacat tcctggcggc acatcctggc atcacccccc acactcacgc ctggggcagc
    76201 gggcaagaac tccgaatccc gtctgaacac agccgctcca agagcgagga atcgtatttt
    76261 ttcgacgaag acggaacctt ggtcggcatg ctgttcgtgt ttcgttccgg actcgatctg
    76321 gccccctaca agacgctgcg atacaccctc tcccggctga aacccagcct ggagttctat
    76381 ttgaccgtag cgcagttggc cgatcgccag aacatggaag ccagtaccat ttatgacacc
    76441 ggcgacgaaa aaacgaccac ccgatatctc gtgctaggcg atcgcagcga ccaacgcctg
    76501 ctcgaagcct cctttacggt cgatccctac gtgaagctct tttctcccta tcgcaaggag
    76561 tttcttgacc gcctccgcga taccgctcaa cacaccggcg gccagcacct cgatacgcag
    76621 gggtctgagg acaaggaacc cttcgcatcg ctgcaacagt tcgcccgtgg ccaaaccgcg
    76681 caacttgcct attgcggcac aaaaaatcag gtcatcgccc tcgacgccta ccagaaagcc
    76741 agcgcctcgg gattcagcgg cacggtctgg caagcggagc ttcgccaccg gttgggcgtc
    76801 tcctgggaag cggcgggcaa cttggaaaag gctaaaagcg aactcctcgc ctccttagcc
    76861 ctacgcccca attccccgga agtcgtcaac aaccttggtg ccgtgtactg gaagctgggc
    76921 gagaagaaaa atgccctggc ctccttcgaa aaagccatat tgatgcgccc taactacgcc
    76981 attgcccgct tcaacgtggc cgaggccatc gcagatgaca acccgcgacg ggccattacg
    77041 gaatacgaaa cctacctggc tctcgtcgaa ggcattctcg aagaaacgga ccgtgccgcg
    77101 ctcgcacaaa aacgcgtgca agcactcaag caccagtagg cctatttgga ttgcagagtg
    77161 aacactcgcc cggcatggag ccgaacgtga gaaacgtggg gacagatcag cggggggact
    77221 tttcggcttc ttcctgactg gtcttacact caatgcataa cgtggtcaca gggcgggctt
    77281 tcagacgctt gtagggaata tcgccttcac agccctcgca tatgccgtag gtttgcgtgg
    77341 caaatcgccc cagcgcctca tcgattttct tgagcagttt tcgctctcgc tcccgaatac
    77401 gaaacgagaa atgctggtcg gcctcagcgg aagcctgatc actcacatcg gggaatactt
    77461 ccaaaccggt cggattcgtc aacaccacgc cggcttcagc caaaatcgcc gcacgctggc
    77521 cttcaaggtc cgccaggata tcgggatact tgaccccgtt tgccttgggt ggctttcgcc
    77581 gagctaccgt ggtttttttc gcgggagtct tcatcgaata ctcggaatcg agatgccggt
    77641 attgtcttca gaccgtatac agaaatttga ctatagcagc cggaaatcaa aggggtcaat
    77701 cggttcttcg aggcttcgcc gaggcatcac catgcccgag ccgaaaaatc ccggtcagag
    77761 atccctccgg ccctcgatcg acttgacgag ggtcacttcg tccgcatact cgatgtccat
    77821 cccgacagga atgccatagg cgatacgggt cacccgaaca tgatgcggct tcaagagccg
    77881 ggtcaagtag atagcggtcg cttcaccctc gatcgtggga ttcgtcgcca cgatgacctc
    77941 ttccactccg cccgctttca ctcgatccag caactcctcg gcacgaatgt ccgatggtcc
    78001 gacgccatcc aacggagaca acactccgag caggacgtga tacaggcctc gatagccccc
    78061 ggcccgttcg atggcgtaca acgtgctggg ctcttcaatg acgaggatct tgctgcgatc
    78121 ccgcttggga tcccggcaga actcacagag ttccccctcg gcaatattcc ggcattgtcg
    78181 gcagaacgac agtccgtcct tcaccgcctg aatcgcctcg gcgaggcgca tcgcatcttc
    78241 ccgttcagcc ttgagcagat gaaaggccag ccgttgcgcg ctcttctgac cgatcccggg
    78301 cagacgcacc aactcacgca ctaacttcgc caacagaccc tgctgatcaa cactcataac
    78361 gcatcatcac atccgggaag cacgccgccg ccatcaaaac aaaccgggga tcttcatccc
    78421 gcccgtcaca gccttcattt cttccgccat catttcacgg gattttttca gcgcatcgtt
    78481 gccggccgcc acgaccaaat cctgcagcat ttcgacatcg ccggccttca cgacctctgg
    78541 atcgatcttc acactcagca ggtccatggc cccgttgacc gtgaccgtca caatcccgcc
    78601 acccgcagtc ccggaaacgg tcttggtcgc ggcctgctcc tgcaccttgg ccatctgttc
    78661 ctgcatggcc tgggcttgct tcaagatgtt ggacatattc ccgaatggac tcttcattcc
    78721 tgtacctcct gcgccggagc cgtcgtgcga acttccgcca gctctccgcc gaacatctcc
    78781 agggcttgtt taacaagggg atgcgccttc gcctgttgcg tcaagatcag gcgctgttcc
    78841 tgctccttcg caacccggat ctgtttcatc gtaggggcgg tccctggatc ctgctccgcc
    78901 acctcgacga ttcgaattcg tacggcacac ccgtagagac tttctcccaa cgccgccatg
    78961 gcttgtaggt tgtcttcctt ctccaacatc gaccgcgcgg tcgtggcctg cttggcaaac
    79021 cccaacgtga tcaacccacc ctcgatcttg accagacgac ccatttcaag gaagggcgcg
    79081 atattgggat gattcgtaga gaccgcctct tggaactgct cccaattcac ttcgaccgcg
    79141 acagccgacg cctcggcagc agaggtcacc ttgggttcct gctgaaccgg cgcagcggaa
    79201 gccggaggaa ccacaggagc cgaaggggca acagcagacg tgcgagcagg tggagccgcc
    79261 acttcgcgcg gcggtgcgac agagggggcc ttgggggtac tcccaccagc agctgacgtc
    79321 tttgcgacgt tgtcctggct gactttcaca ccagtagttt tgaccacagg cgcaggagac
    79381 ggtgtcgcct tatcctgagt ttgcgcaggc gtctgtgtca cgatccggcg gtcggctgcc
    79441 ggcttctcag gctgaaccgc gaggcggctg gatgccggtt ggacttcagc ggttcgcaat
    79501 agccgggtgg cacgaaccgc cgccgtttcg agcgcaaacc gcggatgggc actgactcgc
    79561 aaactgtctt ccgcagcggc atacattcga aacaattcct gtaattgctc gacggtgaac
    79621 cgctcggcgt cacgagccag ctgcgtcaaa tcttcttctg tcgcttcgat gagactccgc
    79681 aactcaggcc ctgacggcac caccgccgcc accagcatat tccgcacata ttccaccaga
    79741 tcggcgcagt aagcgcgcac gtcatgtcct tgatccaaca acgccgcgat gacctgcaac
    79801 gccttggcac tgtcctgttg gatcaccgct tcgaccatgg cccgtacacg ttcctgcgga
    79861 accgcgccca gcaacgcttc gagatcttcg tggcgaatcg tgttgccacc gaacgcaata
    79921 atctgatcca gcaagctgag cccgtcacgc atgctgcctt cgctggcccg cgccagagcc
    79981 atcaggctgc ggtcttcgat ggtcaacccg tcttgatcgg cgacatgccg caaccgctgc
    80041 acaatctcgg ctttggagat ccggcgaaaa ttgtagtgct gacagcgtga caggatcgtc
    80101 gcgggaatct tatgaatttc tgtcgttgcg aagatgaaca ccacatggga gggtggctct
    80161 tcaagcgttt tcagcagagc gttgaacgcc gaattggaga gcatgtgcac ttcgtcgata
    80221 atatagaccc gatactgccc acggaacggc gtaaacttga cattctcgcg aatctcgcga
    80281 acgtcgtcca cgctggtgtt cgaggccccg tcgatctcga ccacgtccac cgacgtgccc
    80341 tgcgtaatct cctggcaatt cgaacaagta ttacacggtg ttcccgtcgg cccctgctcg
    80401 caattcagcg ctttggccaa aatcctggca accgtcgttt tccccacgcc gcgggtaccg
    80461 gagaagagaa acgcatgggc aatccgcttc gtcgcgatcg agttcatcaa cgtctgcacg
    80521 acgtgggact gcccgatgac atcgtcaaac gtccccggcc ggtattttct tgcggaaact
    80581 tggtagtcca tgttgcgtcg atcgtgtcgg ggcggtggtg ctcgtcagtg ctggcggcgc
    80641 ctgacgcgcc gcgcgagatg tcatcgaaga agtggggcca ggtgatcctg cggcacacag
    80701 aacgaaccgc ttaccgttgc ttccttccgg acctggcggg gttcacaggg ttctgttgca
    80761 cagggcccgg ccccgttacg gttacgatat ctatcccatc ccctcgattc gcgaggcgat
    80821 ggtcgagcaa acgttgatgg cggatgaggg ttggcattcg aagtcgcgct cacgcgctcc
    80881 cactgagggg gcccgccccc tcgtgtctcc ccacgcaggg gagtatctcc cctgcgatcc
    80941 ccctcgttcg aatcccaccg cgtccgttcg cgatggcaat tccaactcac acgccttctc
    81001 aaccttgcag ggctctgttc gatggcggag agggtgggat tcgaacccac ggtcccgttg
    81061 ccgagacgca tgctttccaa gcatgtcgat tcgtccactc tcgcacctct ccgcgatcgg
    81121 acgacttcaa ggggacgcat cttatcacgc aggttttggt gcggcaaggt aagccggagc
    81181 ctgcaaacat gacactgcga tggtgaaggg aagtccgacc tcgatcggga gcccccccga
    81241 ggaccccgtg gcgactgccc ccgggatgcc accattgacc actcctgcgc gcattcctat
    81301 actgcgggac tatttcagcg gaggtacgtg atgaaaatcc tggtcgggat cggtgtcgtg
    81361 atcctgctgc tcatcgtcct gatcgtcgcg ttaccattcc tcatcgatct gaacaagtat
    81421 caagaccggt accgcccctt aatcgaagag gccctcaacc ggaaggtcga actccaggac
    81481 attcggctga ccatctggcc acgcatcggc gcaagggtcg gcaacttcgt cgtgcaggac
    81541 gacccggcct ttcgcacagg ccccttcgca tcactctctt cactggatgt cggcgtcaag
    81601 ctgctgccgc tgctcaaggg caaagtcgac gtcgaggaga tcgccctgcg cgacccggtc
    81661 atcacggtcc tgaaaaatgc ccagggccaa ctcaatatct cgaccctcgg tgccaaaaca
    81721 ccggctccgc cggccccctc gaagcctgag gcccccgcgc aggcgcccgg gagtcccctc
    81781 caaatcctgg cgctctttgc ggtggaccgg gtctcgatcg acgggggaaa actgtcgtat
    81841 cgtgacgagt ctaccccgaa accgaccgag tacaccgtca ataatcttga gtttctgctc
    81901 acctcggtcc acctcgacga aagccccacc gtacatctgg gtgcgagggt gcagccctac
    81961 aacttgccgg tccggctcga tggcaccttc ggtccactcg ttgaaaccct ggacgtgaag
    82021 tcgttcacgt tcaacctcgc actagggaaa gtggccgtcg ggctcaaggg acgcgcggtc
    82081 ggagggaacc tggacgccac cgtcaatgcg ctgcagatcg acacggcgga tctccccatc
    82141 gcactgccgc tgacgaaacc ggtacagctc aaggatgtgc atctcaccct ccatgccctg
    82201 tacccgattc cgcccgacac cccgcccgcc aatcaggtcg atctgaccga tctgggattg
    82261 accctggtca tgggcggatc ggcgatcaac gtcaaaggca cggccaccaa aggcttggct
    82321 aatctgacgg cggcatccgc cagtatcaac tccgcggatc ttccggttgc catccccctc
    82381 acgaagccgg tcgaactcaa agaccttcat gtgaacctcc gggccaaata tccgcctaag
    82441 gagggagccg cgccgctcga acttgccgag attccgaacc ttggcctcac cgtcgccatg
    82501 gggagctcac gtatcgaagt caaaggatcg gtgcttggcg gattggcgaa aatcaccgcc
    82561 aactcaaaac tcatcaacac gactgaccta ccaatcgcgc tgacactgaa gaagccggtg
    82621 gaggtcaaag acctgcaggc cgcagccgaa atgaagggac aggaagcacg cctcaccaac
    82681 gcctcgctcc aactgttcgg cggattcgtc cgcacgcaag gaacgctcgg cctgggttcc
    82741 gccactccac cctttagcgg cacggtctcg attcaaggcc tccaactcgg accggccctt
    82801 caggccatcg gcaccgccca ggtgtccatc agcggaaccg ccaacgccga actggcagtc
    82861 aatgggcgtg gatttaccca tccggacctc gtcaaggccc tcgcaggtac cggccgtgcg
    82921 gcagtgaaag aagggaaaat cgaggggatc aatttattgc agcaggcctc gatgttgctc
    82981 aaagtggtcg gcgtttcctt agacaacgtc aaagccaccg ctttttccac catcgaaagc
    83041 gactttgcga tcaaacaagg gctgatcgcc gtccagcgac tcctcatcga cagccatgat
    83101 tttcaggcca ccggcggcgg gacgatcggc ctcgatcagt cgctcgacat gaaactgaat
    83161 ttgaatctgt cccaggcctt gagccagaag attgcggccg gttctcccat cgccagagtc
    83221 gcactgaccg gcgggcgtct ctcgctgccg ctgctcatta ccggcagcac gcaggcccct
    83281 tcctatgggc tcgacacaaa aatgtttgca ggaaaagtca aagagcaggt gaaggaaaag
    83341 gtcaaaggcg ccgtgggaga cctcttgaaa ggttccgcaa aaccggacga tctgaaacag
    83401 caaggcaagg acctcttgaa ggggctcttc gggcggtaac agttgcgggg aggacgcatg
    83461 tcgttggcca atctggtcac tcaatacatc gtgcaatacg gctttcgaat catgggggcg
    83521 atcctggtcg tcgtggccgc cctggtcgtc gccaaatcgg tggggcggct cttcgaacgc
    83581 tggctgaacg agcaggacct ggagccaccg ctccgcctgt tgctcctacg cctcgtgaag
    83641 gccctcgtgg tgatcctcgg gcttctcatc gcgctggatc aactcggcct acaaatcgcg
    83701 ccgctggtcg ccgggctcgg cgtggccggc ttggggatgg gcctcgccct gcaaggcgtc
    83761 ttgagcaatg tcgtcgccgg tctgtcgatc attttcacga aaccctaccg ggtcggcgaa
    83821 catgtttctc tcttaggggt gcacggggat gtcgcaaaaa tcgatatctt cacgacgacg
    83881 ctcatgcatc cggatcactc ccggatcgtg atccccaatc gcaaagtcgt cggagagatt
    83941 cttcacaact tcggcacgat tcggcagctg gatctctcca tcggggtgtc ctaccaaacc
    84001 gatatcgaca ccacactacg cgtgctgcgc gacctcgtca cccgacaccc caaagtactt
    84061 caggacccgg ccccgatgat cgggattgcc ggcttcgccg attcctccat taccctgtcc
    84121 attcggccct gggtccaggt cgcggccgta ggaacggctc acagcgaact caaccaggca
    84181 atcattcaac gattacaagc tgatggggtg gacatcccct tccctcagcg tgaggtacga
    84241 ctactgaatc catcctaagg acagagccgc tgatcaccct ccaggctgca catccacgcc
    84301 gccggtaccc ggcctgactt gttgaccccg gcgcaacatg ctagtctagg cccctttagt
    84361 gatagaacga gcgaccaagg agaccccatc atgagcaggc agacggacct cgatcgcccc
    84421 cgacgctcag aacgaacctc tggcagcagt ctcgccgaca cagaaacgga atccgtgctg
    84481 accgaggagc atgcagccgg caccaacctc gacaagatcc gcgacattct gttcggcgcc
    84541 caggtacgtg atcatgagcg ccggttcgcc aaactcgaag ctcagctgct ggcagaagca
    84601 gcccaattgc gcaacgacct caaggaacga ttcgcctcgc tggagcaata cattcgaaaa
    84661 gaagtggaca cggtgacggg ccgcctgact gccgaagaac aggaccgcac cgatgcggtc
    84721 ggacgcctga ccacggaact gcagacgctg gccggcgtgc tccagcagac ggcgactcag
    84781 ctgcgggagc agagcgagca ggcgcatcac gatttgcgca cccagctcca gaatcaggca
    84841 acatccctct ccggtgattt cacccagcaa cacgccgcct tatcggccac cctcgacgat
    84901 gcggtccgcc aactctcaca ccaaaagacc gaccgcgcgt cccttgcgac gttgttccag
    84961 gagctctcac agaggctctc gcatgaccag cctccgcagc aggcctaact ccacgccgat
    85021 tcgtcagcgg ttccggtctg cccttcagac cgcgcgtacg gctgttttcc cgacaacgat
    85081 gtgtggcgta gaataccccc cttccttcat gccacggaga cgggatggcg cacgcaccta
    85141 aagccggcac gccggcagtc gaacgcgact atgccgaatt gcgcagcctc ctcctggcgc
    85201 ccgagcaatc gcgtctggag caactgcagg agcaggtcga acatctcgac ctcaacgccc
    85261 ataatctgaa tcgtgtgctt cccgaggcga tcgcgcttcg tggggagacc gaccaccggc
    85321 tgacccatgc actcacccct cacgtctccg aagcgctggg ggtctccgtc cgcaaacagc
    85381 cccacatgat cgtcgatgcg attgccccga tcatgatgcc ggcgatccgc caggccatcg
    85441 ccaatgccct gcgcagcatg gtgcagtcgc tcaaccaaac cattgaacac agcctctcga
    85501 tccgaagcat gcagtggcgg ctggaggcct tgcgtaccgg caaaccgttc gccgaaatcg
    85561 tcctgctcca taccctctgt tatcgagtcg agcaggtctt tctcattcac gcacagaccg
    85621 gcctgctgct cgcgcacgca gccggcgacg cggtcgccgt gcaggatcag accctggtct
    85681 cgggcatgtt gtcggcgatt cggagcttcg tccaggattc gttcggcgca acgcccgatc
    85741 aggcgttgaa caccttgcaa gtcggtgatc tgaccgtctg gatcgaacag ggcccctcgg
    85801 caatcctcgc ggccgtcata cgtggcacgc caccggagac gtttcacgtt cacctccaag
    85861 acacgctcga tcgcattcac gccgaacatt ccgatgcgct gacgcgcttc attggtgatg
    85921 cggcaccctt taccgggacc acaccgctcc tggaagaatg cctgcgggcc cagtttgaaa
    85981 cgcgccggcg tgccatcgct cccattacgt gggtcctcct ggcggcgatc gtcctggcag
    86041 caatctggtg gggagtgtcg gcctttcaag accgccaaca ctggcaggcc tatctggacc
    86101 ggctggcctc ggaaccgggc ctcgtcgtca cctccacgca ttcagcaggc aatcggtatg
    86161 tgctcaccgg gctgcgcgac ccgttggccg ctgatccgga cgtgttattg caggacagtg
    86221 gggtggccgc cgatcgagtc gaagcgaaat ggagccccta ctacgccctc gacgccgcgt
    86281 tcaccctgaa acgagccgga atcgtcctgt cacccccaga cacagttcgc ttgaccctcg
    86341 aaggaccgcg cctcacggcc accggcacag ccagcgcaga gtggatccgg cagagccgac
    86401 cgctggcgcg cctactcccc ggtatcaccg aatatgacga ccgccgcctc gtcgctcaat
    86461 cgcttgaggc actggtccaa cggatggcag gggtctggat tctgttccaa cagggaacgg
    86521 cgacgatcca atcaccggaa caacttcagg gggtgcgtcg catatcagag ctcctgcatc
    86581 agctggacga tacggcgcat ctctccggcg caacggtgat actagagatc accggccaaa
    86641 ccgacgtcgt gggccggtca agtcggaacc aacggctgag cgaggatcgc gctcggtcgg
    86701 tactcgatgc ccttcatcct gccacctttt ccgcgatcac gtttcacgct cgcgggatcg
    86761 gcccggcacc tgaatcgtca ccctccctgg gtgccgccac gctgccgcag gaccgacgag
    86821 tgtcttttca ggccactgtt caccccgctt catgaagacg cctctgacat gatcgaaaaa
    86881 aaaatctgca tgctgggcgc ctttgccgtg ggaaaaacca gtctggtgcg ccgcttcgtc
    86941 accagctgtt tctccgaaca gtatcaaacg actatcggag tgaccgtcga caagaaaacc
    87001 atgtcgatgg acggccagcc cgtgacgttg gtgctctggg atctctacgg agaagacgaa
    87061 ttccaaaaat tgcgccgctc gtatctgcgc ggatcgtctg cgtacctggt agtcctggac
    87121 ggaatgcggc gggccacgct ggacatcgcg ttgcacattc aactgaccgc ggcggacaca
    87181 ctcggcccgg ttccgtttgt ggtcctcatc aataaactcg accgccagag ggattgggaa
    87241 gtgaccgacc aagacctcgc gcagttagcc caacggggtt ggacggtgct cctgaccagt
    87301 gccaagacag ggcagggtgt cgaagaggcc ttcacagttc tcgcccgcgc catgctcgcc
    87361 acatcagaac aaacacctcc cgcgggaaca accgatggcg cataacaccc acccgtctgc
    87421 accggaccta ctctgcacgg atctgttgca acggctccaa tttgccatcc tcgaacacgt
    87481 aaccggtaca gagttccgca tcatcgggca cccgccggcc tggctattca ccctctaccc
    87541 cgatgcccga cacaatcggc cactgtccgt cgatgctcgc acaccggtgc ttcagaattt
    87601 cctggccgat gcagccgatg catggcagac agacggcgac gacatcgcgc actctgggtt
    87661 ttggagcgag cagaccgcgg ccgaggagcc ctggcacttt catgccatgg cgctgcgaca
    87721 cggtcccagc cgtctcttgc tgatccaaca aagcacagca gcctatgacc aacaggccac
    87781 cctcctccag cgggcccgcg accaggtact tcagcagcac gaacacaatc gtggacacca
    87841 acgcacccag cacgagctca ccacgaaact ggtggatatg gagcggtcgc gggacgacgt
    87901 cgccgtcatt ttgcagcagc tcggactggc caccctgctc atcgaccaac agggacaggt
    87961 gcggtttctc agtgcctccg cagctcgcct gctggacacg cccgcaaccg gcgggccgaa
    88021 cggcctgctg tgggagacac tgctcccgct gacgaaaccg gaccggctgg ccctgcaatc
    88081 gatgttgcag caacccgcac cccggcgcga acgagtgcag tgccatgtcg aaacccaggc
    88141 cgggcggcac ctctggctgg agatcgaact gcaagacgat ccacgggaca ccggaaccaa
    88201 aatcatgttt ctccacgaca tgaccgacgt ccaccacttg cggcgcctgt tggagctgaa
    88261 ggcgcactat catgatttgg tcggcaagag ccgcgggatg acgcagatct acgaacagat
    88321 tcaggatctg gcccgtgtcg actccacggt cttgatcgaa ggcgagaccg gtacggggaa
    88381 agaattggtg gcccgcgcgt tacaccaggc gagcgcacga cataaagggc ccttcattgc
    88441 ggccaactgc gccgggctca cggattcgct gctgggcagc caactcttcg gccacaagaa
    88501 aggcgccttt accggagcca tcgacgacca gcaggggctc ttcgaggccg cgcagggcgg
    88561 ggtcttgttc ctcgacgaga tcggcgatat cccccacaac gtccaaacca acctgcttcg
    88621 cgtactgcag gagaaggaag tcactcgact cggtgaaacc aggccgagaa aagtgaacgt
    88681 gcgcgtgttg accgcaaccc accacaattt gagtcaggac gtggcgaaag gaaccttccg
    88741 cgccgacctt ctctatcgga ttcgagtctc acggatccag ctcccgccgc tgagggagcg
    88801 aaaggaagat attcccctcc tcgtcgcctc cttcctcacc gaggggcggg ccagcatggg
    88861 gaaagtcatc catcgcgcca gcccggcagc gatggcggca ctgatggagt accattggcc
    88921 cggcaatgtg cgggaactca agagcacgat cgagtgcgcg atgattcatt gcaagggcga
    88981 catgctcgaa gccacagatc tgcctcccga acttcgccag ggaccgacag accatccctc
    89041 cgaagttgtg ctgcacggag acgagcgcag tcgtttcgtg gccgccctcg gacaagcccg
    89101 tggcaaccga acgaaggccg cgcgcctcct cggaatgagc cgcgccacgt tttaccgccg
    89161 gctgagtgaa ctcgatctcc cgaccagcta acctgtcccg cagccactcg cccccactca
    89221 ctgtctcgat atgtctcgtg cgacacagcg cgagacaaaa gcgtttgtct catacgcaga
    89281 caatcctgtg agcttcttaa gggtcctcac ccgaaaaccc agattttatc gtcgcgcagc
    89341 aaaacgtaca gtgtatggca tggagcttga taggagggtt accgcgaaaa ccactgcgtg
    89401 caaaaccctg aagacgggcg acgcagcaga cctatcgaag cgatcgcaag gagaaccgat
    89461 ggataagctc atgcccttct cacgaccaca accggatgcg cccacggcaa gagagcgcga
    89521 gatactcaat cacatttggg ccggcctgac gagtcaggaa attgcgacgc ggctacagat
    89581 cgcccccaaa accgtggaat cacaccgtgc caatctcttg agaaaattcc gcgcgacgaa
    89641 ctcggcacaa ctgcttcgcc tggcactgct tgagggcata ctccacatgc cgtcatcgac
    89701 tagcaccggc ccatcccaga ccgcggcgga cgggcagagc accacagcgg tacggaagct
    89761 gacgaacctc aaatgacgct ctcagctgcc acacccatga ctggatcacc gttgcgcatg
    89821 tgcacagcga ccgcaccgga agtctcagac tcacaagcct gtcgcatagt gacccactcc
    89881 tccgcgccat cggccctgct gggtccatgc gctcaccaga cggcatgaca ctccgctgtc
    89941 tacgtatact caacaggacg agccattcat gcccgtggaa aggctgtcct gcgctaggcc
    90001 cacaattcaa gccgaacagc ggcacgacgg atttctcgcg ccaaaatgtt gatctcctac
    90061 gcagaatcaa ttatacatcg cgaacattgc tcactgatca ggcaaccgac acactgttgc
    90121 cgttcgcaag ccccaggagg aggacctgaa cgatgcgacg acttggaacc ctctgttttg
    90181 cgctacttgt catggcgctc gccgcctcaa tcgcgcgagc agaagccccg gcaccggccg
    90241 atgcgacgcc cgcgctcgat gccgtcacgc tcaaggacgg cacggtgatc tatgggcagg
    90301 tccttggcat gatcgcggac gaactgcaca tcaaaaccag tttcgggccg accgccggtg
    90361 acgatatcgt caagatcatg tggccgaacg tcgccaagct cgtggtcaat cgtcccgtcc
    90421 ccttcagcct caaagaaggc acgacggtag tgggtacggc acaaccaggc gagcccggca
    90481 ccatgatcct gaaagccgcc cccacaggga caccgatggc cattccactc gatgcggtca
    90541 tcggcatgaa ccagcctgcc gtgatctata ccggcgccct ccaggctgga ttttcacaaa
    90601 cgaccggcaa cagccaccta cgaaatggca gcttgctcgg ggaactgtcg gctcgcagtg
    90661 aatcactgcg cctgacgatt ctcggacggt acatctacgg cgataacgcc cagaacctga
    90721 tcgtacggaa cagccgtggc accatcaagc ttgacttctt cctctcgaaa cgtttgtatt
    90781 ggttcgcgtc ggcgtatttc gaacaagata ccttccaaga cttgaagttg cgcaccgccc
    90841 tcgctaccgg tccgggctat caactcctcg accgcggcga cctgacaggc ttcttcaagg
    90901 atatgaccct ctgggcggaa gcgggcgccg cctacttcaa cgaagatttt aagctcgccg
    90961 acgataaatc cagcactcgc ggtcgatggg ccgtgaaatg gaattggccg ctctggggcg
    91021 gcgaccaggt cagcctgtac cacttccagg aaggattcca atcgctcgcc aacgcaaaag
    91081 atctgtacct gacggccgac accggcctgc gatttaaagt gtggggcggg ctcgtgagcg
    91141 gctttcagtg gaccatgcgg tacaacaaaa acccgccgcc gggcgtttcg gatacggaca
    91201 acctctacct gatcacgctg ggatacagct tcgacaccag ccggaaacag taagggccga
    91261 aacgggctcg gcacagagtc ggttcacaca aggaggtggc aggatgagca tgatgagtga
    91321 atttaaagag ttcgccgtaa agggcaatgt gctcgatatg gccgtgggag tcatcatcgg
    91381 aggtgcgttc ggaaaaatcg tatcgtccgt cgtcagcgac atcctgatgc cgcccattgg
    91441 tctcctaatg gggaaagtgg atttctccag cctctttatc cccttgaccg aggatgccaa
    91501 agggaaatcg ctggccgcag ccaaagccgc cggtgctgcc accatcaact acggcgtctt
    91561 cttgcaaacc ctgctcgatt ttacgattct ggccttcgtc attttcatgg tgatcaaaca
    91621 aatgaaccgc ttcaagaaaa cggcccctcc aggcccgccc cctgcgccgc ccaaggaaga
    91681 agcgttactg acggagattc gggatctctt gaagaatcaa cgccgctaat tcgcgctcgc
    91741 gcaggactca tccccgctgc ctgggataaa cccggcagct ccgatatttt tgaaggagga
    91801 ttgcatgacc acactccgta ccattcctgt actgttgagc accctgctgc tcgcctcgtg
    91861 cgcaacggcc ccgtccccta acgactcggc cagccgagcc ttgaatgaaa tgaaagccgt
    91921 cgccaaaccg ctcccgccca aaccagaccc gaaagatgca cgaattgccg aactcgagcg
    91981 gcagaaagca gctctcgaag cggaactggc acaggttcgt tcttccctca ccggcgatct
    92041 ggaccaggca aaagcccgaa ccagtgcgct cgaatcacaa ctcagccagc gcgaccagga
    92101 attagcctca cttcggaacg cctcaggcga caaggagcga ctggccagcc agttgtcgga
    92161 tgtcgaacgt cagctgtcag cgaaggacca ggagctcgcg gccctcaggg gcagcgccgg
    92221 agacaaggac cgccttgccg cacaactggc ggcactccaa gggcaactat ccaccaagga
    92281 tcaggagctt gccggactga aagggaacgc gggagatcgg gaccgcttgt cgtccgagct
    92341 cgcgcaagcc aaacaacgga tcactgaact ggagcgacaa ctggaggcca agcatcacga
    92401 actggcagcg tcgaaaaatg ccgcaggcga ccgggaaaaa atcgttgcag acctcgcagc
    92461 ggccaaacag cgggcctcgg acctcgagag tgagctcgcg cgcagagacc aggaaatggc
    92521 ttccctcaaa ggcgcgttgg atcaacaaaa aaccagtctg gcggaagcaa aggacgattt
    92581 atcgaaactg ctgcaggcgg aagtggccaa aggcaacgtg accatgaagc agttgggaga
    92641 tcagctcacc ttggggcttg ccaccacgtt gctgttcgac tccggcgaag cgacacttaa
    92701 gccgggggga gccgatgtct tgcaccgcat cggtggcgtg ctcaaacaat accccgatcg
    92761 ttcaattcat gtggccggcc acaccgataa cgtgcccatc aagggcagac tggccaagac
    92821 gtatccaacc aatgtcgaac tgtctcaagc gcgcgccgaa agtgcgcggc aggcgctgac
    92881 cgaggggggc atggccccgg acaagatcga agccaaaggg cgtgccgaca gccggccgat
    92941 tgcgagcaat tcgaccgccg aaggacggca gaagaatcga cgcgtcgaaa tcgtcgtcgg
    93001 acactaaccg gctacccgca gctccaccag ccttgcatca gccccaggaa cgcgatagat
    93061 tgctgtcgtg ttcttggggc ctgcatgtca ctcaacagat tctgtcggga cgcatggctc
    93121 acaccaactg atcatcatcc gtcagctcgg tcacagtcgc cttcttttcc tgactgcggt
    93181 ggcttctctc gggcgactgt cgcccccatg caaccggcgc cgtcttccta tcaatgaccg
    93241 gggagagatc gtatgagacg agcagtatgg gcgctcatgg ttctcagttc cctcgccgca
    93301 ggctgtgaaa ccgctccgca cgtccagccc atcgcatcga gcccagcgac cgtagaaccg
    93361 aagctgctgg cccttcaaca ggagcgagaa cagctgttga ccacgctggg tgaattccac
    93421 gatcgcattc gcgacctcga aagcaaactg ggagaccgcc agagtcaacc cgccgcggca
    93481 tcatacgacc aattgctcag cgcgaaggaa gcggaccttg tcgaactgag gcggttagca
    93541 ccggaacgcg atcgactcac cgtccaattg acgaccgccg ccaacgagct catccaggct
    93601 cgccaacgaa tcgggacgct tgaacaacag ctggccacgc gagacaaaga tctggctgcc
    93661 ctccagaccc gggcaacggc cgtcgccgac ctggaagtcg cccgacgcag gatctcggag
    93721 ctagaagcgc acatcgcccg tcaagaccgc gatctccaca cagttcgtac ggggtccgcc
    93781 gaacgggaca gcctggcggc tcaactacaa accgccaccg ccaccatcga ctccttaaaa
    93841 gcccgtatca gcgcactcga tcagcaactc aaagaccgcg agcaggccta tgaaacggtt
    93901 cgatcgcgcc tcatggaacg cgacaagcta gtgccccaat acaatgcgat gattgcagaa
    93961 atttaccagg ccagacaccg catcgcagcg ctcgaacagc gcctgaacga gaaggggcga
    94021 gatctgtcgc cgcggcagaa ggggactcaa ctcaccacca gtcagcgcga cggaggaccg
    94081 ctgaaccagc gctccggcgc tgccaaccaa tctcttggcg acacggccaa atcgataccc
    94141 gatgggaaat cagcccccca gggaaaggac tccacacgca gcggtgttgc atttccagga
    94201 aaccccgctc aggctgaggc tcggagcgga agctttgcgg cggtgaaaga agaacttctc
    94261 aaagtcctgc cgggcgacaa tagacagaaa acgatcagca tgaggcagga tggaaatcga
    94321 ttaacggtcg cgctggacag caacttgctg ttcacatcgg gtgacgctgc gctgagcccg
    94381 gaaggcgcaa ctatcctcaa acgcatcggt gcggtcctgg gacagctttc agacaagttc
    94441 gtgcaggtcg caggacacac cgacaatcaa gcactcagca aaacgcttca aaaaaccttc
    94501 cccgacaata aggcgctctc atgggctcgc gctgaaaatg cccgcagggc actggtcaat
    94561 ggagggatac ccgccgacag gacgaaggcc gtcgggctgg ccgatacacg cccgctcaca
    94621 tccaatgcca ccgagcaggg caggcagaaa aatcgccgcc tcgaattgat catcgtccag
    94681 gggccgactg ttgccgcgac ggtcgaggag tcgaggcgcg ggcaggttcg actcgcgacc
    94741 ctcagcgcaa caccttagcc tgtcacacaa acggaatcgc cgcgtactga cgactcgcca
    94801 tcgtcacatc acggcctgtt cgctacaact gacagacacc agcgtgaaca ggcgccgcaa
    94861 ggcggtcggc tggaaccgtc gccagaatct tgtaatgccc aagccccagc tcctttccgt
    94921 aggcttcccc gaccagcaca tcctgcatat gctgatggtt tgaggcctgg aagtaggacc
    94981 gtccctcttt cagcccgtca aactcgagcc cctccaacgc cttaataagt gcggcagcgc
    95041 ccgtcgttcc tgctcgttga atcgcctcaa ggacctgcgt catggcggca tacccaagat
    95101 agcagcgcga agttggcgtg tgattgtatt tgtcgacgac tgctcgaata aactgcctgg
    95161 atccttccgt gttgaccttt ggatcccaga tgagccccca aatgccggcg ttgttggcat
    95221 aaccgagggg ccgcccaatt tgttcacccg caatcatccc acccacgccg atcttctcct
    95281 tcgcaaactc aagtttagtg tatgccttca gtgcgtggac taaatcccat ccatagagat
    95341 tgagcaccac cagcgtcgga tgtttgtcct tggcggccgt gaacgccgca gtgaagtccg
    95401 tcgccccgaa aggcactagt gtctccccca caaactcaac gccatgagcc tggccggccg
    95461 tcaccatggc ctgcgccatg gctttgccgt ccaatgagct ggtcgtcagc atataccagc
    95521 gggtaccgta ggccttgacc aagtggggca cgacagcctg cgccatcatc gaggcattcg
    95581 gcataaacac gaaggtatgc gagctgcacg cggctccggt cagttcgggg agaagggagc
    95641 ccgtgaccat gaacagcttg ttctccttct tcgccaactc cgaaaccaca agcgcacaat
    95701 ccgcattaaa cgtgcccatc aggacatcaa cctgatcgtc ctgcacaaac tttgtggcag
    95761 ctttgatcgc cgtctcagga ttcgaggcgt catcggcctc gagaatcacc accggccgcc
    95821 ccaacacgcc accacgttta ttgaagagat cgacggccac attggcacca tgaacatcat
    95881 gaatggagga ggtcttgtac gggctcgaga gcggatcgag aattccgatc ttgatcggct
    95941 ctgcggcaaa cgccactgcg tgatcggacg acgcaccgag cacacggtcg gccaaatcgc
    96001 taaaaagcag cgcgccgccg gtcatggctg atagttgcaa gaaacgtctg cgtgagatcc
    96061 tattcataca cttgatcccc cctggttttc attcgccctc ccaggcgcca cgaccccgat
    96121 ggtaaggcca tgatcacacc gagagggatt gtctctctta acagagtcat ctatgttttc
    96181 acagcacgaa gttagacctc ggggacgacg agctcctctc acaatgggca agatatagcc
    96241 ctcgcgagtg atgctcgtca gaacaactgg gggagccggg caggaatctt cctggagtgc
    96301 gggggattca catatccttc gctagttgcg gcacgaggac gaagcgggac tgagaacacg
    96361 catctcagcc tccatcccgg taaccgcggc tggaatgtac cgggagagtg tggatgacgg
    96421 gttagctgta ctggtcttcg gtgtagacct gcttaatcga ccagaatact gcctctttta
    96481 acttcttgat ctgagggtct gggggatcgt tgcggatctt ctgcagtttc ctatcgagtg
    96541 caaacagtgg ctcaaccgat cgccggtcgg gaacccgccc caatgcccag gcaatctccg
    96601 tctgaacagc gatgtcaatg ccaggcgcgt ccaacatctc aagcagcggc ggcaccactc
    96661 tcgggtcact atgggtgttc ccaagatcgc ccaacccctt agccgctgct gcctgcaagt
    96721 caggctgttt ggagtgcttc aggatctcga tgagcactgg gattccctcc ttccccatat
    96781 tgcccaacgc caccgctgct tgcttcgcca agtccttatc cttcagcgcc tctgtcagat
    96841 gaggcaatgc ttcatcggcc accatctctc ccaggaggga aataatcttc aatttccgga
    96901 gctggctggt gtccggagaa tccatcaact tcagcagtcg atccggctgc ccccattcgg
    96961 ccgtcaagaa gagcggaaat tcatacttct ctaactcgac ctggagcgcg gtcagggcat
    97021 agggaccggg cgcaccaccc tgagccgatt gagcggcctg attggcatct tcaaattccg
    97081 tccgaacctc cttctgccat ttgatcttca tccctcccaa agcatatgtc acctgacacg
    97141 ccggtccctt ccacaggcga gaaggggcat ccagtaaatc attgtctcca ccggccgccg
    97201 cggtaccttc ccaagtctta cgttgctcgc actttacccg gacgacgaca tcgtgcggaa
    97261 gggcgggatc cgcaatggct gcatagccca tttccttcat acgtcgcgtg acaacttctc
    97321 tgattccctc ggggttacca gcgcccgtat cagtaagcgc gatcacctca acgaggaccg
    97381 tctggatatt tgccagttgc gccttttgcg tttcactaaa gtagtcccga taggcccatg
    97441 ctgtagagcc gatgccaagg agaagcagaa ggaagatagc tgcggcaaag gaagcgctcc
    97501 gcgtcatatg gacttcctcc tttccaatag ggtcttgatg aaggtccatg caacaaagaa
    97561 tcgacacttc attatacgct ccaaggcctc ccgactctac gaaggaacgt caagcatcga
    97621 cctgaaccga cagcccacag tcccccgccc agccacgtta tcctcagcat cctttttcca
    97681 ctccgaccga caggccaaca cgcaggcccc acctgcgtat gggttgacag gcagactcgt
    97741 tcgacggtac aaaatgtcat tattcatcgt gtctttccac gcgcaggcac attccttatg
    97801 tagcatcggt gagcaaccta gtactcagcg tcgcgcgaga gtggcggaac tggcagacgc
    97861 gcgagactta ggatctcgtg ggtaaccgtg ggggttcaag tcccccctct cgcaccacct
    97921 ctcacagatc cgtatggcgc aaataacttt tcggacccgg aggacgtgaa cagctttatg
    97981 aaaatggaaa tgaccgaact cggccccatg aagcgggccc tcaaaattga agtcccggca
    98041 gatgaagtga acctgcgctt tgttcaggcc tactctgaat tgaaccggca ggttcgcatt
    98101 cccggattca gacccggcaa ggctccgctc cagttacttg agaagcgata tgcaaagacg
    98161 atcgaagagg acgtgattcg cagcctggtc ccggattatt atgatcgagc catccgccaa
    98221 gcgggcatcg ttccggttct cgtagaaatt ccgccactgg aacgggtcaa agtcaaaaaa
    98281 gacacgcctt ttagctttac cgcaaccgtt gaaatcaagc cgaccatcga gcttcgagat
    98341 tacaaagctc cgaatcctat ctccctcaaa caggatcaga gaacggtctc ggatgaacag
    98401 gtccacaaag ccctcgacgt tctccgcgag caaatggccc aattacatcc tgctcccgcc
    98461 ggaacaacat tggccgatgg cgactatgcg gtcttagata ttgaaggaac cttggatggc
    98521 aagccactcg aaggaacgac caaagaaggg caccttcaca aaatgggctc acatgcctcc
    98581 gtccttggca tcgacattga atctcatctc ccaggtaaaa aggaaggtga ggcgataact
    98641 attccacaag cctatcccgc gacacaccct gatactcgcg tcgccggcaa gaccgtgagc
    98701 tttcgctgta ccatcaagtc cgtaaagcag aaacagctcc ccgcgctaga cgacgagttc
    98761 gcaaaggatt gcggtcccta ccaatcattg caggaaatcc gagagaagct tcgcaccgaa
    98821 atggaacggg ctctgaagaa agatatcgag gatagctaca aggacaccat cttaaagcga
    98881 ctggctgaaa cccatcattt tgaacttccc ggaacactgg ttgagcgaga gctgtcggcc
    98941 atggtccggc aacaattgca atcccgccag cggaaatccg gcgacaatcc cgacacttca
    99001 cctaccgcgc ctcaaacaga ggacgccaag aaactccagg atgaactgcg cccagaggcc
    99061 gaacggcgcg tcaaggttgg acttatcctt gaggcactgg cggcgaagga aagcatcacc
    99121 gtcacgaatg aggatcttgg caacgaaatc acgaggctcg ctgcggaggt caaactctcg
    99181 gtcgaagaag tcacccgcat gattcaagcc ggtgggcaag acaccctaga cgaccttcga
    99241 tctcggattt tggccgataa agctttggat tttgtctaca agcacgcaat gatccagggg
    99301 tagcatagtc ggctgccatg agtcgcaaca ctggatgccg ggcggtgatc accaaaatcc
    99361 aggttaattg agtatggtaa actgaagagt ttccacctgt caggcctcta gcggaaagga
    99421 ttcagctgaa tgctagttcc gattgtcatc gaacagacga accgcggcga acgtgcctat
    99481 gacatttact cacgcctgct taaagaccgc atcatctttc tcggcgcgcc gatcgatgac
    99541 gtctttgcaa acctgattat cgctcaactg ctgttcctgg aagccgaaga ccctgagaag
    99601 gatatcaatc tgtacatcaa ttcccccggg ggaagtgtca cggctggact tggcatctac
    99661 gatacgatgc aatacgtcaa acctgccatc aatacgatct gcttaggcca agctgccagc
    99721 atgggcgcct tcctcctgac agccggcaca aagggcaagc gctacgcctt gccgaatgcc
    99781 cgagtcatga tccatcagcc gatgggagga tttcagggcc aggccacgga gatcgatatt
    99841 cacgcgcgcg agatcctgaa gattcgcgaa cgcctgaacg aaatcatggc taaacacact
    99901 gggcagccac tcgacaaaat ttcgcaggac accgagcgag actatttcat gtcgagcgaa
    99961 gaagcaaagc ggtatggatt gatcgatgaa gttatcacac gccccctgaa gagcctcaag
   100021 cccgtagggt ccacggactc agggaaggac ggcggcaaga gctagttgac ctttggtgta
   100081 caggaggcag catggctaag caagaaaaga ttgatcggca cctgcgatgc tcgttttgcg
   100141 ggaaaagccg tgacgaagtg cgtaagctca tcgccggtcc caccgtctac atctgcgatg
   100201 aatgtgtcaa cctctgcaac gacatcattg cggaggattg ggaagaggct aaggaagaaa
   100261 tatcttcgaa gctgaagaaa cctgccgaaa tcaaacacca ccttgatcaa tacgtggtgg
   100321 ggcaggatcg tgccaagcga atcctctccg ttgccgtaca caaccactac aagcgcatct
   100381 ccgccaagga gaaagacgtc gacgatgtcg aactccaaaa aggcaatatt ctggtcatcg
   100441 gcccaaccgg caccggaaaa acactcctgg ctcaaaccct ggccaagttc cttgacgtgc
   100501 cgttcaccct tgcggatgcc accacgctca cagaagcagg ctatgtcggt gaagacgttg
   100561 agaacattat cctaaaactg cttcaggccg ccgactacga tgtcgaacgc gctgagcgcg
   100621 ggattgtata tatcgacgaa atcgacaaga ttagcagaaa aagcgatagc ccttccatca
   100681 cgagggatgt ctccggtgaa ggcgttcagc aggcgctcct gaaacttatt gaaggaaccg
   100741 tcgcaaacgt gccgccgcaa ggtgggcgca agcacccaca tcaagagttc attcaggtga
   100801 acacctcaaa catcttgttc gtctgcggcg gggcatttgt cgggcttgag catataatcg
   100861 aacaacgcat gaacagaaaa tcgatggggt tcggagcgga agtccgaggc cgcaacgaca
   100921 tccgattggg tgaattgctg ccgcatgtcc aaccggagga tctcctgaag tatggattga
   100981 tcccggagtt catcggccgc ttgccggtcg tcgccaccct ggatgaactc gacgagcagg
   101041 cgttgattcg catccttacc gagccccgaa atgcgctcac caagcaatat gaaaagttgc
   101101 tttccttcga gaaggtcaag ctccgtttta cagagggagc cctgagcgcc gttgcacgga
   101161 aagccttcac acaaaagacc ggtgcacgcg ggttacgctc aattctagaa gaggccatgc
   101221 tcgacgtgat gtacgatgcg ccttcgcaga aacaaattaa ggaagtcgtc ataaccgaag
   101281 acgctatcag cggaaaacac gctccgatcc ggatctttga gcaggataag gacgtgaaga
   101341 gcgcatagct ttcccgtggc agtttattct caccactagc ttactccttc cagagggggc
   101401 ctcgcgcccc ctctttttat gctcccccgg ggcagcactt caggcgtgcc ttcaccaacc
   101461 cactccccaa atcttaggtt tttgtcgaca agaccatttc gtgcggtata cttgcggaaa
   101521 gatggatatc ctcccactgg tgccttttcc gaagggcggc gcatgtgaaa ttcccagtcg
   101581 tatccagcaa tagacacaag ggcaaaacgc ttgagattga ttcctccaaa gaagtgatca
   101641 ccttggtggg catcaacggc gagccaatgg gaagcctggc ctgggacttt ctcgtcgatc
   101701 aaattctgac ctaccggaaa cctcagcagt cccgtgaggc gcgatctgag cctcgcatct
   101761 cactctcaat acgggtcaaa taccacaccc ccgagggcac tcaattcgaa agtcgagcgg
   101821 gcgggatcgg cggaggaggc ttatttatcg agagctttac gcccttagcc gtgggaacta
   101881 agctcgccat ggagtttact ctgccggaaa gtccgggcga gtggttgtcg gccaaaggcg
   101941 tgatcgcgtg ggtttgtccg aaagccgacc aatatacatt tagcccggga atgggcgtcc
   102001 gcttcagcga catcgcggcg gaaacacgga atcgagtact cagcctggtt cattccgtca
   102061 agagcatcac ccaggacgaa ccaaagtaaa caactcctat acaacaccgc aattcgaaac
   102121 tccccaatac acacacaaga gatctgagcc aaggatgaag tttcccgtta ccgccactcg
   102181 cgaacaccaa gggaagacgc tcagcattga ctgcgaccaa gaaaccctgt ctctattcga
   102241 tgacaccggc caacgcctca gcatacttac ctggggcaca ttgatcgaac acatgctctc
   102301 ggtcgacgaa gacgctcgct tcgcccatag ccgtgcgcat ccccgagcgc ccctcgcaat
   102361 caaggtacac tgcaccaccg gcgatggaaa acacttcgat agtctgacgg gcgggatcag
   102421 cggaggcgga ctatttattg aaagtagtgc accgttagca cccggcactg aattgaaggt
   102481 tgaatttgcc ttgcccgacc ggccaagtca gaaattcgaa acgcgcgcga aggtggcatg
   102541 ggcacgttca aaacccgaac gatacttatt attccccgga atgggcattc agttcaccga
   102601 tatcgacccg gaagcacaag cacggctcat tgacttggtt gcctctctga accgaactcg
   102661 tgcgacagcg taatctgttg cacagcccat cgagcccatc cacgaaccat catatctgga
   102721 tccgcctcag ccgccctcga cagctccgcc aagaccccgt tatcaccgat tttgcctaac
   102781 cgaaacgcgg cctctgcgcg aacgcttgcc gccccatctg tcctgagaag tgagcgaagg
   102841 tgggaaacgg caccaggatt cccgctctcc cctagcacgg ccgcgactcc ctgacgcaca
   102901 cgcgcatccg gatctcgaag cgctttgatc agatcagcgg agagcgacac ggtatccaag
   102961 ctgagcaacg cacgagttgc ggcaatacga gcagaaccct gcggatgccg aagaaccgca
   103021 agagtctgag cctcacgatc cgcagaaaat tcaatttcac cgagagccag cgccgatgcc
   103081 attcgaaccg gctcagcaaa atcggccaaa tgccctgcga gcgccatccc actcttcccg
   103141 tcacccagcc gacccagtgc gagagccgca gcttcgcgga cacgagcgtc cggatcattg
   103201 agcgccacga tcagactggc attggaagac ttatggccga ccttccccaa agcctctgcg
   103261 gcagtgcgcc ggacgtccga gtctgggtcg gcaagcaact caacgaggcg ccgcgatacc
   103321 gtctccggag cccccgatgg agtatcgacg taacacccgt acaccacgga gcacagcagc
   103381 agccagaaga agaattggag tcgaaaataa gaacgcatcg gggaagccga acgtatatga
   103441 gtgagagaat tcaacggaat tgaagcgtcc atcctagcgg gaagaaaaga acctaagccc
   103501 gcacgtaact acgaaattgg gatgcaggtt cgagatgagg cacttgggtg gatcgcccag
   103561 gataccaacc agcaagacgc gagcaaaaaa gaaccggtcg acttcctaat tcgacgaggc
   103621 cccggaatca cccgcgcagg ggaaaagaat tattagcctc tcctacacgg actttgcgga
   103681 ggcatcgtca tccaacacag gtgcctgctt ggctgcctcg atggactgtt ctagttcctt
   103741 ctgcgccgaa tccatttcag cccggattgt tcgcatttcg ggatcgaccg aacttcgcac
   103801 atccgcaacc gcctgacgga aggtgcgcac ggcatccccc aatccctccc ccaaaccctg
   103861 catgtcctgg gctgacaggc ccgacggttg agcctgagct gcctttgttc gcatggctgc
   103921 ctgagccgcc tgcacccgcg ccacggcatc cttgttcaca atcgccgacg gacgcttagc
   103981 caccggcttg ctctgggcgc ctggcggaag gggcggatac tgcgcgcgca tcactggggc
   104041 aggagccgcc atacgctctt ccatcgtcgg aggctggtga ctttgtgcaa ccgccgaccc
   104101 cgctgccgct tgccccggcg tgaccgtcgc cacacgcggt ttcgttggtt gagtcgcttg
   104161 cggcccttgt ggcccagccg cagccatcag agccgctgtc gtacctggag tcagctcagg
   104221 tcccggcgtg tatggagagg tggctttagg agccgccgcc ggcgctacgg tggcgggctg
   104281 cgcgacctgt ggaatatctg ccgccggctg agcctggatc tgagcaggca tggcagcggt
   104341 ttcgttttgg tcgggagcgt tctcgggagg tggaatatca ttcacctcct tcttaaaccc
   104401 tttgagcgcc ttgccgaccc cctcgccgat ttgcggaagt ttcccggccc caaaaatgat
   104461 caacacaatg accaggatga taatcaactc ggaaaatccc atagtgccaa acatgttcga
   104521 tatccttcct cggcagatag gagtcaatcc agcgtcagag ggcctcttcg gccctctgaa
   104581 ctcccacgat agctgcccga tgcaaagggt gtcaagaaag gtgggcggcc acagccggcc
   104641 caggaatagc accgaagaac ctagctcgca gggatgggaa tcgggagaag atcggcctga
   104701 accggctggc cgtacaccac cgcgtcgcgg gcggagagat acacatccag ttcactccga
   104761 ataccgaact catccggcag gtagatgcca ggctcaatcg aaaagcaggt gcccggcaac
   104821 aaccggcgcg catcctgcgt ctccagattg tcgatattcg cgccgttgcc atgcacttcc
   104881 tcaccgatgg aatggccggt tcggtgcacg aaatattgcc cgtaccctgc gtcctgaatc
   104941 acccggcggc agacatcatc cacttcccac ccataggggc attcaccggc tcgtacacga
   105001 ccttgcacaa aggtgacggc cgcatcccga gcccgtcgaa caatctgaaa aatgtcctgg
   105061 tgccgggcag gcacagtcgc cccgacgaac cccgtccagg tgatatccgc atagaccgcc
   105121 cctggtccag gctgtttagc ccacaggtcg atcaatacaa ggtcaccctg ccgaatcggg
   105181 gctgatcctt cggaagccgg cgcgtaatga ggatcggcgc tatgcgcatt caccgcagca
   105241 atcggcggac tcgaggttac caagccacgg gcctgcatgc gggaaagaat gtattgctgt
   105301 aatccatatt cagtcaggct ggaccgagca gccagcgaag ttccgacaaa tccaaacgcc
   105361 tcgtcgacaa ttgcgcgtag accttcggct gccacctgat gcgacgccaa ttgtgcatca
   105421 tcccacaccg cctcaaattg ctgaaccaga tcagcagagg tcaccacctc cgcgccgaga
   105481 ctgcgcacca gatcaatcgt gccggcatcc acgcgtgaca gatagggaat ggcattcatc
   105541 ggcgaatatt gcatcgcgat ccgcttggca gggtgcagca gggaaccgag agccgcctgc
   105601 tgatcgcgcc aagagacata cgcatgcgcg tctccgggca acccatccag gacatgaggc
   105661 tcaatgcgat gctggagctt caccggcgta ccctgcgcgg gaacccaata gtaccaacgc
   105721 cgcgtgacat ggagcgaggg gtctaacaac aagacgcgat acgcgatggg gtcgaggtgg
   105781 cgaaagtcgt aaaagagcca cccatcaagt cccggctgct cccggatggc ctgctgaata
   105841 cgaatcaccc gtgcgtgaga acgatcggcc acgttcatgc ttgccatcat agcgtcattc
   105901 cgccatcgat accactgggc aatcacgctg ttctctgccc tccacgcaat gttagaatga
   105961 gccgaacctg ctatggctat cgttccctca tccgatcccg cgcagtaccg cgtcaccttc
   106021 ttcttcgggc cggaagccgt cgaagagcga ccggatcatc tgcgatgcgt cttcaacgtg
   106081 aagaagcgga gctggaaagg cggcgtgcaa gtggctgtgg atgtggcccg gcgtcacctc
   106141 gaccgggccc gcgagcgaat tgggtatgcc tcctggctgg ctgaactcat acacagcggc
   106201 caccaagagg agcgggagga gctgaccagc cgcgccgatg atctcttcgt ccagtcgctc
   106261 tgtaccagtg cgctcaactt ggccctccag gcgggaattg aacaggtgaa tcaacagatc
   106321 gacgccgcca cgttcgccga cgaactgcat caactcctcg acgaacaacc ggaacaggtg
   106381 accgatcgaa ttcgcctcga actggacctg ggtgagactt ccgaaatcta atgagcaacg
   106441 gtgtggcagc aaagccggaa ggggtttgaa aacggggggg aatttgttat aacgacaacg
   106501 gactcatcgg cattcatttt tctttccacc gcatcagaac ttcagcacga aggaggcacc
   106561 caacgtgaac gtctctgtct attcgcccct taaaagacga cgcagcctct gggctcaagt
   106621 cctcgtcttc agtctggtca cagtctgttc tctggcttta ctcatcgccc ccgtctccgc
   106681 gcaggagtcg ggaggcgcgt ctaatgtgcc ggtctcgtcg aacgatgcca gtccgtcggg
   106741 tgtcggcatt caagcgggct ccgcactggc caccatcgtc tatttcccgc tcaaactggc
   106801 cttcgcgatc ggcggcgggg tcgtcggcgg attggcctat ggcttctccg gaggcagtga
   106861 acagacggcc aagaatatct ggatccccag catgtacggc acctacatca tcacgccgga
   106921 tcatctcaag ggcgataaac ccatccgctt cctcggcgtc tcaggggagc ctgcgcgaga
   106981 ggccggcgat tacgccccgc aagaaccagc gccgctgcct ttggaaccga tccgctagca
   107041 tgaaacccgt catcggagtc acccccgact tcaatgccgg tgatcgtcaa gaatggggcg
   107101 gcaaggagcc cacatatttt ttgcgggccc ggtatgtccg cgccattgag gaactcggcg
   107161 gcgtgccggt aattctgcca cttgtggcga accgatccgc acgccggcgc ctgctggaag
   107221 gggtcgacgg actcctcctg acaggaagcg ggccggactt ggatccaacc ctgtacggag
   107281 aaaaaaagcg atataccttt ccggtcgtcg ctaaacgacg ctccagcttt gaattggact
   107341 tggtgcggtt agccatccgg aaccacatcc ccacgctggc tatttgcgga ggcatgcaaa
   107401 gcatgaatgt cgcctgcgga gggagcctgt atcaggatat tccggcccag gtcgaccatg
   107461 tgttacagca ccgacagacg acaccggcgg ttcgactctc tcacagcatc tccatcacac
   107521 ccggcagcct gctcaatcgc atcgtcaagc gcgcccgcat gcaggtcaac agctcgcatc
   107581 accaatcggt gaaagcagtc gggcacgccc ttattgccag cgcaaccgcg ccggatggca
   107641 ttgttgaagc gatcgaactt ccgacgcacc ccttctttct cggcctgcaa tggcaccccg
   107701 aatttttgtt cgaacgccac ctcttgcacc gacgactctt tcagacattc ttgcgggccg
   107761 cctcgcgccg cccgtcactc caaccttcgc ctgcgagacg caggaacaac tcatgacacg
   107821 gggttcattt cgccataaca gcggacatgg acgtggcgcc gccggagaga ggacactgcg
   107881 tgggcaatcc gctgtttcgc accaaatcca tcgaacgaat cctcgctgat tccgacgccc
   107941 ctgaacatcg cctgaaacgg accctgacgg cctgggacct gaccggactc ggcatcggcg
   108001 cgattatcgg caccggcatc ttcgtgctga tcggcaccgc tatcgtcggt gatgcgcacc
   108061 ggccaggcgc ggggcccggc atcatgctgt cgttcattct ttcaggcatc acctgcgcgc
   108121 tcgccgccct ctgctatgcc gaatttgccg ccatgatccc ggtcgccggc agtgcctata
   108181 cctattctta tgccaccttg ggagaatttc tggcctggct gactggctgg aatctgatcc
   108241 tggaatatgg cgtggcctgc gtggccgtcg cgatcgggtg gtccggatac tttaacaaca
   108301 tcctcaaact ctgcggcctt gaactcccat actgggccac tcatcctccc ggcgcggacg
   108361 gcggcattgc gaacattccc gccgcgatca tcgtgttgct cgtcacctgc atcttgattg
   108421 tcggggtgaa agagagcgcg cgcgccacct gcggcattgt gctcatcaag ctggccgtga
   108481 tcgccttctt cattgcggtc ggcacatcct ccgtcgacac ggccaattgg tcccccttca
   108541 tgccgttcgg atttgccggc gtcggcgcgg ccgcagccat cgtgttcttc gcctatatcg
   108601 gattcgacgc tgtgtccacc accgctgaag aggccaagaa cccccaacgg gatttaccca
   108661 tcgggatctt cgcctcgctg gccatctgca ccgtgctcta tatttctgtg gccgcgatcc
   108721 tgaccggatt agtgccttat tcgcagatcg acgttcatgc cccggtagcg gaagggctcc
   108781 gcatggtcgg attcaagtgg ggagccgcca tcgtggcaac cggtgcggta gcgggcatta
   108841 ccagtgtcct ggtggtgatg atgctgggcc agattcgggt ctttttcgcc atgtcgcggg
   108901 acggtctcct gggtccctgg ctttcaggcg tccatccgaa gtttcgaacg ccccaccacg
   108961 ccacctattt gaccggcgtg gccgtcgcca tcatggccgc actcattccg atcggtgagg
   109021 cagcggatat gacgaacatc gggaccctct ttgcctttgt cctcgtctgt atcggcatca
   109081 tggtcttgcg ctacacgaag ccgaaccatc cacgcccctt ccgtatgcca ttcatgccgg
   109141 tcgtccccat tttgggagtc ctggcctgcc tgggcctaat gtatttcctg ccctggatga
   109201 cctggatccg gtttttcgtg tggaccatca tcggcgtcct agtctacgcc acgtacggga
   109261 tgcgccatag caaactcgcc acaaggccct cgacagaaca gtccgagaca cctcgaccgg
   109321 catagtcgaa ttacgagcca aaaaactggt gacgggaaaa actaggacga tgcttcggtt
   109381 ggagcaaccg gggaggttgt cggaggtgct gctgcagccg gggccgcagg cttttgggca
   109441 actgccgcag gtggaggaga tgctggcgcc ggagtcgagg tacctgctgc cgcaggagca
   109501 ggctgagcag ccgcaggctt gggaggagca gggggcttcg gcggagcagg ccgctccggt
   109561 ttaatcccgc cttcccagga aggacctgaa tacatatcgg cggccagccc tttgcctttc
   109621 cacttttctt cgaaatctgc agccgctttg ttcggggttt ctcccgcacc caccacgtca
   109681 ttccagggat aggcccgggg accaatctgg cacccggtct ccgtacgcct caaatagagg
   109741 gcaatcgggt tcccccggta cggcccgaga tagatatgca ctgaccccac atattcgagc
   109801 aatgtggcca ccgcgcctcc aagaagaggt gcatgatgcc ataagaggcg ggaagagggc
   109861 aagatggacc tatcaccccc ctgctatttg gtccagaagc gattaggtcg taacggggcg
   109921 ctctctcgta gggcgcatgc gcagcaggta cacccccaag atgaggacga tgataacggt
   109981 cacgttgagg ccgacatcca tcacatactg ctggaagagt tcctgccgcg cctctcgcgt
   110041 caccacctca agctgatggg ataattccgc gctggccgtc aacacccgtc gcgtgcaggc
   110101 gatgatgccg accgccagat agggctccaa ttccaacacc tcggtctgaa gaaaccgcac
   110161 caccgtacgg aacaattcca gcaggatgat gacgagcagc agatcgttca acaacttgag
   110221 tccggcaggc agcagcggga catcgttgga gcgcaagaaa aaggcgtacc aggcatgaac
   110281 aaaaatcagc atgcctaagg ccagcaagct gaatcccgcc gcgacatagc ccaaccggtc
   110341 taagtgatcc atgaatccca gcaaccactg catggttgcc cggcgagact tctccaaagg
   110401 cccgggaggg gtcaccgggg gcgtcatgct gttcctgcct gaccggtagg catgggggaa
   110461 tcctccagtg gttgaccgaa tttcaaacca ttgaccgcct tggctgtgat ggtgtggcca
   110521 caacaatcaa tcccctgaaa cccggcaccg ccgtcgatca ccatcaggcg caggccgcag
   110581 gagtcgggga actgccgctt ttcctccagc atagccccgg ggagcaatgc gccttttttc
   110641 cggcctctcg acgagatgat atccggcacc acatcttctt cagtcagccc atgcccgcaa
   110701 cagaggatct tctggaaacc ctcgccgccc ccggccacct tgaccaccaa cccgcacgaa
   110761 tccggaaagc gctttcgctc gtcgagaatg tcaccttttt tgattgccat gccgcctcgg
   110821 tatgaaagga aagacgtgaa acgtcacatg attcgatagt caatcagatc gccgggggaa
   110881 aagccagcgc agactctatc ggccgagccc aggatatgga gagcctggat cgcgctgtag
   110941 aaatcaggcc ggcgccatga attgaccaga aaaagaacac ccactccccc cgtgcctgga
   111001 gtgagaggcg aacggaacag ggcgcacgca gatcagacgt gcgtcactgg agaatcatgg
   111061 gctggtgccg cctgacccct ctcaactaac gtcggtttgg ggatgcgcgc ataggccttg
   111121 tgccatgagt cgagcagcac cttgtgcgac cggcacccaa ccaccaaccg cgcatcggaa
   111181 atcgtgaccg gcttgtaggg atggaggtac cgtgccttga gcttcgccac acgggccagc
   111241 gactggtcca gcctctcctg agtgaccctg ccatctttca ccgcacgctc catcgcttgc
   111301 atggcggtca tgacacggtc ctggtccttg cagatcaaca ggacatcgca tccggccaca
   111361 aacgaccgga ctgccgcttc gccgatcccg tcgtgatcga taatggcatg catctccaga
   111421 tcgtcggtaa agaccacgcc ctcataccga aactcttcgc gcaacaagcg ctggatcacc
   111481 gccgaggaca gggttgccgg tgcctccgga tccaaggccc gatacagcac atgggcggtc
   111541 atcaaactgg ctacgccata acgaatggcc tgctgaaagg gaggaaactc cgtatcgcgc
   111601 aaccgctgga gccccgcatc cacgacggga agctctttgt gcgaatccgt cgccgtgtct
   111661 ccatggccgg ggaagtgctt cccgcatgcg accaccatat tgtcctgcag ccctccgatg
   111721 gtcgccgacc ccatctcgcc gacaagctcg ggagcagcgc cgaacgcccg atcgccgatg
   111781 acgggattgt ccggattgct gttcacatcc aggaccggag ccatgttcat attgatcccc
   111841 accgcccgca actctttggc aatggtcgcg gcagcggagt aggccagttc actggagttg
   111901 cactgtccca gctgtccgca gggagggaaa atcgtaaatt cggagggcag ccgggatacc
   111961 cggccgccct cctggtcaat cgcgatcagc agcggctgag cgggcgacag tttctgcagc
   112021 ccgttggtga gatcgacgat ctgctgaacc gactcgagat tgcggcggaa aaagatgacg
   112081 ccgcccggct tggaggctgt cagaaacgag gccaggtctt tggtgaccgt ggtcccggtg
   112141 aaacctatca taaaaagctg accgatctgc tcgcgtaatg tcatcactcg ctactccgac
   112201 gatctggtga gaagcaggca gatcccccgt tgcgttcacg taccgaaggc aggtcctacg
   112261 gcaacgtgcg gtccaggagg aactgaatga ggaggcgtgt gccgatcccg gtcggaccct
   112321 tcggcagata cgctcgctca cgttcgctcc agtcggtgct cgcaatgtcc aaatggatcc
   112381 atgggcaatc gccgacaaac ttgctcagga acaaggcggc ggtgatcatc cctccgcccc
   112441 ggccgccgat attccgcata tccgccacat cgctgcgcag ttgttcgaaa tactcctccc
   112501 acagcggcat ctcccacacg cgctcgccgg cgcgctggcc ggcgttccgg acatgttctt
   112561 tcaactgatc gttgttgccg aacatcccga tggcaaattg gccgagcgcc accacacagg
   112621 cgccggtcag ggtcgcgata tcgatcaaca ccgccggttt atagcgggtg gcgtatgcta
   112681 aggcgtccga cagaatcagc cggccctcgg catcggtatt ctgcacttcg acggttttgc
   112741 cggagagggt cgtcacgata tctcccggcc gcatcgcgcg cccgcccggc atattttcgg
   112801 ctacgggcag aatgctgacg aggtggagcg gaagcttgag ccgcgcggcg gcccgcatcg
   112861 tcgccaggac ttcggcgccg cccgtcatat ccgccttcat atgttccatg ttctcggccg
   112921 gcttcagcga aatgccgccg gtgtcaaagg tgatggtctt tcccaccagc accaccggag
   112981 gatcgccctt cttcgctttg gcgcccttgt attccaggat gatgaacttc ggcggttcgt
   113041 ggctgccacg cgccacaccc agcagggcac ccatgccaag ctgctccatg tccttctgtt
   113101 cgagcacctt cagacgcaca ccggattctt tggccaccgc cttggcctca cgtacgatgc
   113161 gcgtcggcgt catcacgttg gccggatggt tgcacaggtc gcgaaccagc accgtcgctt
   113221 cagcagtcgc cacaccgcgc cggatgccct cggtcatttg ggggagcagc gaggcttgcg
   113281 acgtgtagat cgtcaaccgt tcgacgtcga tcggcttgct tccattgggg ctacgatagg
   113341 cggtgaattg atagttaccg agaatggcgc cctccgccat ggcctgcgcc acatcctgga
   113401 ccggaatctc ctccagaatg gcggccggca acacgacggc gaaggaggtc actttcgcct
   113461 gacggacacg tttgatcgca ataccaagcg cctggcggaa cgcatccaca cggagatctt
   113521 tttccttacc caatccagcc aacaacagcc gttttgctct ggccttgccc tgggtatgga
   113581 ccagcaaccc ttcgccgagc ttcccttcga attctccgcg ctgaatcaaa gcggcaagct
   113641 gtcccccgag ttgagcatcg atggcggcag cctcctgcgt gagatcagag ccaccctcgc
   113701 aatggagcaa caccagaacc tcggccgcct cgttttctgc ccgtccaacc tgtgcgtgaa
   113761 cttggatctt cttcatcaat cctcccctgg ttcttgccgt tcagcgttca tcatcagccg
   113821 ctcacagact gccccgtcct taagaatcac acccctctca aacgccgcgc atatccggcg
   113881 cccaaatgta tttgtgcatt tggagctgga accgcaccgg caacttatcg gccagtatcc
   113941 actccgccag atgccgcacc tccacttcgc cgaacaccgg actcatgagc accgtgcacc
   114001 gcgccggaag atcgtaacga tggagcacgt cccgcgccca ttcatagtca gatcgatcct
   114061 tgatcacaaa tttcacttca tcgtgaggtg cgagccgatc gaggttcggc caatacattc
   114121 gatccgtcat cccactcccg ggacatttca catcaagaat cacatggacg cgccgatcga
   114181 caccggccgt atcgatggcc ccgctggttt ccaccagcac ctcgaacccc tcgtcacaca
   114241 ggcgactcag caagggcagc gactccgtct ggctcaacgg ttcccccccg gtcacttcca
   114301 caagatcaca atcgaaggcg cggacctgat tgacgatgtc gtcgatcgtc atatcccgac
   114361 cgccgtagaa ggcataggcc gtgtcgcacc aagtgcaccg gagcggacac ccggtcaacc
   114421 ggacgaatac gcagggacgg ccggcatagg tagactcgcc ctggatgctg tggaaaattt
   114481 cggtaatctt gaccatcgcc gattgactca gcggctcagc atcgcactga tggatgaatc
   114541 gggggactta tttccatctc gtcacaggcc atccattgcg tcgcgcaatg cgatccattc
   114601 cgcgaatcgg attcacaacc aatggatgcc ccaccatttc cagcaactca acatcgccgg
   114661 gactatcgcc ataggcaaac gactgcgtca gatcaatgcg tgattcctgt gtcaaccggg
   114721 cgatcaaggc gcgcttgccg gggccataag gaagggggac acagacctgg cctgtaaaac
   114781 gacgctgttg ccgttccagt ttcgcggcca gcagggtggg cacctccagc aaggtggcca
   114841 acggagcaat gaggaagtcg agggatccgg tcaccagcac gatatggtgc cccgcccggc
   114901 gatgttcgtc catgcgagcg agcccctgcg ccgaaagacg cggaaacagc tcggcacgac
   114961 agaactcctc agctagcgac tcaacctctg ccgcagcctt cccggccaga taaagcttcc
   115021 gttcccgcag ggggtgcaat gacaccggag gcgcattgcg aagcacccag ccaacactat
   115081 cccgcagctc gcgccacccg acaagacccc gcttccagag aaagcgaaaa aaacggactt
   115141 cgctcgcctg ccccgggagc aaggtattgt cgacatcaaa caacgcggca atgtcagtct
   115201 gccggctggt gcccgaaata tgtgagagtg agagaggtgt catctcgatt gttggcctgc
   115261 tgatcgctgt gataaggaag aatctgcgac aggtccgcac agcacgcgtg aattctactg
   115321 gacaactagt tgattgacaa gcattttgga ccacttctat aatgcgcttc ctctgaggcc
   115381 tggcaccggc gtagcaagaa tgcgcgtttc accgccgtct agcttgtgtg tacctcgctt
   115441 catgtttcga cggagagact ctcggttgcc gaagtggtgg aatggcagac acgcacgttt
   115501 gaggggcgtg tggcgcaagc cgtgcgggtt caagtcccgc cttcggcacc aaactcaaca
   115561 gtcgttcgac aaactcaccg cccatcagcg actcgccgca aatacggcac gttcgtttca
   115621 gcaccatcct tcttcaggcg cttcttctct ccggcagggt gcacccgaca cgagcacctg
   115681 caccatccgg ctcattgtgc agccaccagc caggatcgac ggctctgcaa tgactcctct
   115741 gctacagata aacggcccta ctccactagg gcctaattac attctcaatt gtcaatcatc
   115801 tctaaccgcc tactccggct tggtgctatg atgtgcggcg tgcaagccta ttcactggat
   115861 ggtgagtgcg acacttcata ccaaatgaac gttccgccga agcagtcccg ccacccctct
   115921 cgcatatacg catggtgaag gaacgtccac taaggatgca gcccgctcat agcagacgaa
   115981 ggaggcacct gtgactgcgc aagacgtgac aacggccctc ccgccgccgg gagatttcag
   116041 caccgttgag gcccttgtcc gaaccctgtc cggtcaagca tccatcggca tctttctgtc
   116101 ggaccccgaa ggccgcacgc tgtacctgaa tgagcggctg cgccgcatcg ctggacttcc
   116161 cgtcgtaccg acgccagggg aatgctggcg caatgcgctg gcacccgaag accatgaccc
   116221 aatctgcagc gaatggtctg gagccaccaa cgccgagcgc agcttttcac gtgagtttcg
   116281 cttccggcgg ccggacggct cgctgcgctg ggtcatggcg gaagccttcc cattgcgcag
   116341 cggcggagga ccctccggcg gttacgtcgg catcgtgcgc gacattaccc ccagacaact
   116401 ggcactggac gccctgcacg caagcgagga gcgctatcgg acgctggcgc aacagtcgcc
   116461 ccatgcaatc ctcgtccatg cggactctgc catcctgttc atcaatgaag ccggcgcccg
   116521 gcttttcgga ctcgcgaccg cacaccccat cgagggccgc ccgctgtggg atttcttccc
   116581 gaaagagttt ctccacgacc tccccttggc agaagccgcg cccagcccct cagtcgagcg
   116641 acaattggta cgacccgatg gaacggtcat tgatgtcgaa ttgacagcag ccccggtcat
   116701 gttcgatgga catccagccg tccaaattct gggcaccgat atcaccgaac ggaaggtcat
   116761 cgccgggcaa ctgcatcggg ccaaaaaaat ggcgaccgtg gcgaccctgg ccggcggcat
   116821 cgcccatgaa tttaacaatt gcctgaccgc catcatgggg ttctcggact tggcgctgcc
   116881 gtcactcgtg ccggacagtc gcgtccatgg ccacatccag caggtagtcc tcgcgtcgaa
   116941 acgggctcgt gatctcgtca cacaaatgct catggtcggt cgacaggcgg acggcgcgaa
   117001 acagccactc tccctggata tcctcctcaa agaaacactg cgagtcctga agatgaaact
   117061 gccggagtcc atcagcctcc gcgaatggat tcccggcgca accaaccccg tgctggccga
   117121 cccaacccag atccatgaac tttgcctgaa cctgctggca cggtctcaac aggccatgac
   117181 gatcagcggc ggggtcttgg aggtccgcct cgacaatatg ggtcttgacg cttcgatgac
   117241 cggtcacgac cttccgctcc caccaggtca gtatgtgcgc ctcatgatct cggataccgg
   117301 tgacggcatt cccgctgaca tccggacgcc caacatgtat ccacttttcg cacccccctc
   117361 gacgggaagc aaagcgggag cggggctcgc ggagttacag cgaatcgtga gcgagcatgg
   117421 cggaacactc cgcgccacca gcacagccgg ccagggcacc accatcgagg tctatctgcc
   117481 cgcaatgctt cagccccctc agacagtggc tcaggaaccg agccgcgagc gcaccgcacc
   117541 ccccacggaa gcgaaggaat ttcttgccga gcatgacaaa gagcggtaaa ttcactgtag
   117601 taattaaacg gtgtcgaaaa gccgccaccg gccgtctcct gagagccgca acacaatacc
   117661 caaacactcg aacacgaata ctcctgtgag gaatgttatg ccctcggtcc tgatcgtgga
   117721 tgatgaagaa gcgattcgcc gactgattcg cagcacgctg gaacaggcag gctatcatgt
   117781 ccgcgaagcg gccgacggca aagcagggct gtcccactat cgtcaatccc cggctgatct
   117841 cgtgatcatg gatatcctga tgccggacca ggacggcctt gagagtattt tgacgctccg
   117901 tcgtgaattt cccgaggcca ggattatggc gatcaccggc ggaagcgaca tgatcggcat
   117961 cttaaacttc ctcgatgtgg cccgtatgct gggcgcacgc cgcacgctcc agaaaccctt
   118021 cgagatgcag cagctcctgg atgcggttca agccgagatc gccggctgac cgacgcctgc
   118081 ctcatctagt ccgttcgtca taccgcccca ctgtcacatt gacttccggc acatcgatcc
   118141 gcacactagc gcccgctttc cgatcccctt catacataca agaaagatgt gatcatgaag
   118201 acgtggtgga ctccgcagct acacgcggta ttcggcggca tcgtcgtcgc aatcggggtt
   118261 ttcgtcatgt gggagcaggc ggcgaccacc tgggccctgc tcgtcgccac tgcatcgact
   118321 gcctttctct ggtggcaggg acgcagcatc gcggccgtct gggcctggac caccctgggg
   118381 ctcgggatcg aaagcatgac ctggccgatt gtgaccatga ttcgaatgag gatgggggga
   118441 agtcagccga ccgaggagca gatggaaacg attctgaacg ccgtgctgtt cgggctgttc
   118501 acatcggtat tctggatcac cttcgcgttt ggactattca agcgtatacg cgaacagaac
   118561 gccccgccgg caccaccgcc cggaaacggg ccggtcccgg cacggagcaa gaagaaacgg
   118621 gccaggcgct gactctcgtc acgccatggc aaccggatcg acatcgatat cgaaccggag
   118681 gccacctcgt cgtgattctc gatcgagcac tccacgggtc cgcacgacta tttctttgcc
   118741 tgcctcaaga gacagagact tgaccagaat ctgccaacaa tagcgacctc gcgccatggc
   118801 atgtggtgcc ggagacggtc ccaggataac gatgctgctc gtctctccac cgaaaccagt
   118861 ggaacgctgc agcagaggcg aaccgtcgcc ggcattggtc ccccgatttc cggcgctcac
   118921 cacctccgcc cgtagcaacg ccgcccagcg accggcggct tgggcgacca ccggttccag
   118981 cgtcccggac acatccaacc tcacaagcga cgtcaggggc gggtactgaa gcatctgccg
   119041 aaaagcctgc tcctgttgaa gaaacacgga atcgtcacca gcggccaccg ccataatcgc
   119101 atgatgatcc gttaagcggg tctggatcat caccgcgcct ccggcatgag cgggcagagc
   119161 taactccacg gcatccatca ggccatggta catccgttcc gccgagcgaa agtccggcac
   119221 gtgaagaccg gcatccgcat ccggaacccc gacaaacgcc gcgcgtgcct gaaggccaaa
   119281 ccggaacaac atctgcgtgc cgatcacgat gtccaattca ccggcggcaa gcagccgact
   119341 gaacgcccgt gcatcagcag gccggcgaat cgtctcacca tccacacgac ccacccgcgc
   119401 cagagggaac ttccgccgta cggcctcttc gatacgctcc gtacctgagc caaccggctc
   119461 caacttgagc gattggcacc gagtgcaatg atccggcacg ggtttcgttc gcccgcagta
   119521 atggcaccgc acatgattgc tgcgccggaa aaacgtcaat gcaacgctac acgcatcgca
   119581 ctgcggcatc gcgccacagt ccccgcaatg caagacactc gcaaatcctt tgcggttcag
   119641 atagagaatc gccagcgcgt tctgccgcag ggcttcctga atgccctcat agagcggcgg
   119701 cgataacaag gttcccgccg gggtctccct ggagtattcc ctcaaatcga tgacctgcac
   119761 cctcggaaaa cctgacggat ctcggtagac acaggctgtc atctcccctt gctgaaccgc
   119821 cgaccaactc tcaagcgacg gatggctcga ggccagcacc aacagggatc gatcgcagaa
   119881 ggcccgccgc cgtgccacct cccgcgcatg gtaccggggc gattgctcat ccttaaggga
   119941 cgcatcgtcc tcaccttcca cccacaccaa tcccaatcga tcgatgggcg cgaacaccgc
   120001 cattcgggtg ccgaccagga tcgtggccga cgaatcctgt gccgattgcc acactgctgc
   120061 gcgttccctt gccgaaagac cactgtgtag gagcaacggc cggtctcccg ctgccgccag
   120121 ggcttcggcc aatcgaccgg cattctcgac gtcacccgtg acgaccaaca cacgccgccc
   120181 acgacggata gcggcccgag ctgcctgcac aagacaccag agtctggtgg cccgagcccc
   120241 ttgaaccagg aacgagccgt atacgtcctg cgaaagcgcc cggccaacgg tatcaggcca
   120301 cgctgccgcc tccagtggcg gcggcaagtc atcaactgtc aatctcgcga aaaccgcctg
   120361 ctccgccccc ggagaagggg gtttttttgc tcgagccgcc ctcgggacaa ccgcgtcatc
   120421 gcagcgcacc acgagccctc ttcgaatcag gatctgcacc gcgcgcatga cacgggactt
   120481 gtcaccctgt gccagggtac gctctgtaat gcccttggga cgacgacgaa ggcgcgagag
   120541 cacctgagtt tccacttctc ccaagccctc aggtaacgac gaattgtccg tgccctgcgc
   120601 ggtcggccga taccgtgatg gccgccgatc gacctgatcg acaaaaggcg ccacaagctt
   120661 gatgcattgc ccccacggtg cggcataccg gtcggccacc caccggctga gatcgatttg
   120721 actggaggtg agcaggtggt ctggagacgc tggggcaagg cagcggacgg ctttcagccc
   120781 ctgctcggga gcgccgagcg gaaggcggtc atacaccgcc gtgacgagac cgtgaaggtc
   120841 ctgggaccca aacggcacga tcactgattg cccgattgcg acctgcccct tgagtgaaga
   120901 cgggatcaca taggtaaagg gccggtgaag gcggcgcggc agcaccacat cggcatacct
   120961 agggggaagc gcaggagcca ccggtggagt tgcagacgaa cggtgatcga acatgcggca
   121021 ttctaacaac accggatgag cgcaacaatc acggtccttg cgagaagggg gctgacggga
   121081 cgccgtggtg gaagcggggg gctatctgcc aggagcgagg atcaacacac ctgcggcgac
   121141 gggcctcctc ggaagagacc cgtcgcccgt cactcattac gaaccgggcg ggggcgaggc
   121201 cgtctcaccg ccgacaggcg aaaccggcac tgcgacatcc gagggagcgg gagcgatcgg
   121261 tgcatccacc gacgccgatc ctgcctgggt ggctgaagcg ggtggcacga ctgcggtgga
   121321 tgccggtgcc gcgtccagtt ggttatacgt aaactgcgca ggaatcggat cgctctcact
   121381 cagcggcgcc ttcgcggact gggcctgggt gccctcctcc ggcgaatcga gcacaacctc
   121441 gatacggcgg ttttttttgc ggccctcctc ggtcccattg ctggcgacag gacggctcgc
   121501 tccatacccc acggcagaca acttcgccga atccatgccg cccttctcga tcagataccg
   121561 cacgatctcg gcggcacggg ccttggacaa ctcccaattg gttgggaata ctcctttgag
   121621 cgacggccct atctccatat tgtcggagtg accttccacg cgcacctgct gagagaccgc
   121681 agcagtcctc aaatactccg ccagttgttc cagaatgggt acgccggcag gtttcacttt
   121741 cacctcaccg gattcatatt ccaactgatc tgccagatca tcaagattga tcctggtgct
   121801 tccatgagcg cgctcgaccc tgggtttgct catcgaatgg gactcgctcg gcatcgcggc
   121861 agcgctgtcc gtcctggcgt tcatcaccgg ttggaccgtc gcagccggaa catccgagtc
   121921 cccgttctca tattgcatga cctcaacagg cgaaacgtgt ttggagcgaa cgcggtcgcc
   121981 gatcagcgca cgggccgtgg ccttggacaa caccgcggta gccgtgtggg gctcggtaga
   122041 aagaatcttc acttccccga ttttccgctc aggaagccca ccgccggtac ggaagacttc
   122101 cagatattcg ccggaacgaa ggccttcgtc ttgtcccttg tcgagataga ccaggtttcc
   122161 ctgcgacacg agcgacatat gtttatcggc ctgcagttca acaatcatgg cttcgatctc
   122221 ggcatgcccc gaggcggtct caacgacttc ttctgctaaa ggcggtgtaa accgcatgac
   122281 cggatcgcca ggggagaggg gaccatagga ccgaacaacc tggacgccaa ccagtgccgc
   122341 gtctatctga atgactttca ccacccccac tctattgatg atatagccca tgtactgctt
   122401 ggtcatgggg tgaaacactt tgcgcgagcg ccggtataca gtgtagatct cacagatcgc
   122461 cgaatcaaaa aaaaaaaaaa atttgagata gagggtatcg gtcccggacc agcccaacat
   122521 catccggttc ccggagagtt ggttgtcccc ggtaatcaca ttgataaccc cgtcaatcgg
   122581 catctcctgt cgaatcgagc cggcggcata cgtgagggcg gcttccccca ggcgaacact
   122641 ttgagcgttc tgagcatgaa cagggatggc cgtcacagct gcgagactgc aagacaggat
   122701 gatcaggctg gcgcgcatgg gtaatgtcct catattgatc acacctagga gggttatctg
   122761 tagcaacctt agcaaagagc aggggcttgt caagaaacgg gacggcgctg gacggtttgc
   122821 ttcaggtctc cacgggtgct atcgtaagcc tcgcaccgga gtgacgattc ggcaggcatc
   122881 gatagcaggg cgtctgaata gcgaggtatg gcgtgggaac agagaagcgg cgcatcaaca
   122941 ctcaaatgcc ccgcagattg aaggttcgaa ccgccacgcc catggcttca gaatgggata
   123001 tcagcgtcgg atccccgccg agtgcgctaa gaaccccggc cgacaagatg aggacaggat
   123061 cctcgaatag ccaagccacc cccgacacat cgccgacact tcccaagaag aacgcaagtc
   123121 tccccggctc atagccacaa tccgctgtgg cacctaactg ctggacagta ttcagtttct
   123181 aggactttcg ggactagagg cctaggtcct ttgtgcgctt gctattaaaa ataggcaggg
   123241 gctaagattc cgacctccta attagcccta taagccctca aacccgttaa ggttcgagcc
   123301 aatggccggt tccgagctcc tcttagagta cctaagtcga tattttccga tcctgatgtt
   123361 tgtgttcgtc gcactggcgt tcggcgcggg cacattgctc atcagctact tcgtccagcc
   123421 gaagtacccg gaaccggaaa aactctccac ttatgagtgc ggatctgaac cgttttccga
   123481 tgcccggatg ccgttcccag ttcgctacta catctttgcc atgctgtttg tcattttcga
   123541 tgtggaagtc atctttctgt atccctgggc tattgttttc aacaagatcg gcctgattgg
   123601 gcttgtagag atgctgatct tcatcggact tttcctcgtc gcctatgtct acgcgtggcg
   123661 aaaaggagct ttggaatggg attgattcag ctgggaggcc acgataaaga cgggactccc
   123721 gatgtcatta ctctcacggt ggaaaaagcc gtgaattggg cccggaaagg gtccttgtgg
   123781 ccgatgacct tcggactggc ttgctgcgcc attgaaatga tcgccgccgt ctcgtcccgt
   123841 tacgacatgg atcgatatgg tgccggggtc tttcgggcct ccccccgcca gtccgacctc
   123901 atgatcgtgg ccggaaccgt ctgccgccgc atggccccgg ttattcgcaa gatttacgac
   123961 cagatgccgg aaccgaagta cgtgattgcc atgggatcgt gcgcaacatc cggaaacatt
   124021 tacgacagct acagcgtggt gcagggagtg gatcgatttg tgccggtcga tatttatgtg
   124081 ccggggtgcc ccccgactcc ggaggcctta ttcgatggca ttctgaaact gcaagagcgc
   124141 atcatgcaaa agcgtgtgtt cctcgcgcag ccgaaagaag tgaaagacgc cctgaaggtc
   124201 tgacgcacgg aaacgaaccg aatgagtcaa ctggccaaac gtatcgagga tacctttccg
   124261 ggcgcctgca cccaggtggt ggagtggcgc ggggatgttt ccgtcaccgt caaacgtgag
   124321 gccttgcacg acatcggcaa gtttttacgc gatgacccgg ccatgaggtt cgactacatc
   124381 gtccatgtga gttcggtcga ttggccggat gacgaggaac gtttcgaagt ggtctatgag
   124441 gtgtattcga ttcgaacccg gcagcgcatt cgcctgaaaa ctcgggttcc ggagtcggat
   124501 tgtattgtgg actccttgac cgacatctgg aagggcgctg atttcatgga gcgcgaagtg
   124561 tacgacatga tgggaatccg gtttcggaac catcccgacc ttcgtcgcat tctgatgccg
   124621 gacgagtatg aagaaggcta ccccctacgc aaagactttc cgctccgcgg caaaggatgg
   124681 cgcgatacgt ttgaattttt ggacgagccg acccggtagg gcggccattt tggactacta
   124741 aacgctcatg gcacagttcg aagatcaacg aactacaatc tataaagtcg atccggaaca
   124801 tccggaagac gcgacgcttc caaccctgcg aacggaagag ctgctcctca atatggggcc
   124861 gcagcacccc agcacgcacg gcgtgctgaa agtcatcctc gagctggaag gcgagcggtt
   124921 ggtcaagtcc accccagtga tgggctttct ccaccgcggt gtagaaaagc tggctgaaga
   124981 aggcacatat catcagttta ttccgcacac cgaccgcctc gattacgtgt gtgcgatgta
   125041 caacaacttc gcgtattgcc gcgccgtcga aaagctaatg aacatcaccg tgccggaccg
   125101 cgccgagtac ctgcgcacga tcgtcgcgga aatccagcgc attatcggcc accagttctg
   125161 gctcggcacg caggccctcg acatcggggc gatgacggtc ttcttctatt gcttccggga
   125221 ccgtgaaatt ctgctcgatt ggtttgatga actgtgcggc gctcgcctca cgacgagctg
   125281 gtatcgtatc ggcggcgtcg agcgcgattt cacaccgtcc ctgttcgcca agctgaaaca
   125341 gttcctcgac tatttccctc cgaagatcga tgagtacgtg atcttcctgg agaagaaccg
   125401 catctggctg gcgcgcacca aaggcgtggc cgtgatttcg gccgaagatg cactgagctt
   125461 tggcttgagc ggaccgacgt tacgaggctc aggcgttgat tacgatcttc gtaaatacga
   125521 gccatactct gcctatccca agtgtgaatt cagcgtcccg gtcggcaaga acggcgacac
   125581 ctatgaccgg tactggatcc gcgtgcaaga gatctacgaa agcgtcaaga tcatccgcca
   125641 gtgccttgag cagatccagg acggcccgat catggctgac gtgccgagcg tgactctgcc
   125701 tccgaaagaa cgcgtgttca cgaacctgga gtccatgatt cagcagttca aactcttctc
   125761 gcaagggttt aacgcgcctc ccggagaaat ctactgcggg accgaagcgc ataaaggcga
   125821 attgggtttc tacatcgtca gcacgggcgg cgggaagccg taccggctga aaatccgcgc
   125881 cccgtcattt atccatatgg gcgcattcga tcatatgtcg aaaggctaca tgatcgcgga
   125941 cgccgtgacg atcttcggca cgtacgatat cgtcatgggg gaatgcgatc gatgaagacc
   126001 gatacagcac aaaggaaaaa gtgagcggcg ctgattcatt ctttcgcccc atcgcttttg
   126061 acttgaggta acttcatggg tttgaaacca gcaaccaatc cagacgtcga agcggccacg
   126121 atcgaactgt cgatcgacgg acggacggta tcggccaaag atggcgtctc gctgtacgat
   126181 gtcattgcca gcacgggcaa gattattcct gccatgtgtt atcactatac ctttgatccc
   126241 ttcggatcct gcggtatgtg cctggtcatg caggaaggca agaaggcccc ggtccgttcc
   126301 tgcactgcca aggcggcagc cggtatggtg atccgcactg acggcgacga cctcttcgcg
   126361 gcacgaaaga aagccgtcga aaaacatctc tcggttcacc cgctggactg cccggtctgc
   126421 gacgcagacg ggcattgcga gctccaggac atggccttcc agcatggtgt gaccaatctc
   126481 gccaatgcga agcaaaagtt cattcctgaa gacacgcgga gtctcgtcct cgacttcaac
   126541 atgaatcgct gcatcgcttg cgcggaatgc atcaacgtct gcaaagacgt gctgatgatc
   126601 gacgcgttgc aattcatgaa gaagggcgga ttcaaccagg tcgtggcgaa aggggaccaa
   126661 ccccttgcct gtgaattctg cggagactgc ctggctgtct gtccggtcgg cgcgatcacg
   126721 aacaaatatt ccaagtacct gtacaagccc tggcagatga aaaaaaccac caccacctgc
   126781 aactattgtg gtgatggctg ccagatgcat ctcgaaacca aagataccga agtcgtccgc
   126841 gtgacttccc cgctctcctg gaaaaataag tggggcgacc ggactgaaac tgccaaggga
   126901 cacggtggac tctgcgttcg cggccgattc ggattccagt acatcgacag tgaacaacgc
   126961 cttcggcagc ctctcgtccg cacgggcgag acgctgacgg aaacgccgtg gctcgacacc
   127021 atgcaaactg tgatcgatcg attgtccgac attcatcgga agcacggacc ggagtcgatc
   127081 gccgggctga ttaccgcgcg ctgcacgaac gaagaattgt atctgttcca gaaactcatg
   127141 cgcgccgggc taagaaccaa ccagctcgac agcagtgccc gctacggcca cctcaactat
   127201 gttcatgccg tacgtcatgc cgtcggcgtc ggccgtccgc tcaacgactg ggaagatttg
   127261 accaaagcca aagccatctt ggtcatcggc tcgaatatca cggaaactaa cccgctgacg
   127321 gctgtccgca tcaaggaagc catccgcgtg tatcactccc aggtcatcgt cgtcgactct
   127381 gccaacacca acatcgcgca actagcttcg catccgcttt tggtcaagcc aggcagcgaa
   127441 tcgttcttga tcgatgggct ggtgaaatcg gtgatcgaac aggatctgat cgatgaagcc
   127501 agcaccactc ggcacccgca ggccttttcc gcattgaagc aagccctggc ccaggtctcc
   127561 ctcgaacagg tctccgcaca gaccggcatc gcagtcgagc agatccggga aatcgccgcc
   127621 atttttgctg aagccccgcg cgccattgcg ttgtgtgccg aaggcatcgt ccggcagccg
   127681 aacggttacc agaacgttct caagctgatt gatctcgcct gggtgacggg caagttaggt
   127741 caacccggat gcggcgtgac cacggtcaca gaagaaatca atgagcaggg tgcagtggat
   127801 atgggcgtcg caccggaatt cctccccggg caggcacgat tcgatgatcc aggggcaagg
   127861 gaacgcttcg agaaagcttg ggacgtgacc cttcctgcca gcggaagcgg cgctaatttc
   127921 ctggaaattc tcgcccgtat tcgaaacggc cagatcaagg cgttgtatgt catcggagag
   127981 aatcccctgg ccactttgcc cgcctccatg gatgtgaagg ggacgctcga gaagctccaa
   128041 ctgctcatcg ttcaagaccc cttcctcaca gagacagggc aaatggcgca tgtcgtcctc
   128101 ccggctgcca cctacgcgga aaaggacggc accttcacca atctggaggg caaagtgctc
   128161 cgtgtgcgtc aggcagtgga tcaggtcggg gaaagcctgc cggactggca tatcatgacg
   128221 gcgctggcca acggtttggg atatgactgg acctatgaat cgccgcaaga cgtgcagaat
   128281 gaaatcatga agctgttacc gggctactac aatctcggcc agcctcgccg cgtctcgcca
   128341 tcggccgatg cctatttgtc gaacggctat gaggctgaag tcgcgagccg gtatcaccgc
   128401 cagacagcac aatcaacgtc cagcgacgac acgcagccct ttaccttact gaccggacaa
   128461 gtgctctatc attccgggaa aatgtccacg catgcatcgg ggctcatcaa tatcgaaccg
   128521 aacaacgggc gtatacgaat gaatccagcc gatgtggaac ggctcggact gaacgatcaa
   128581 tccaccattc gcctcacctc gcggcaaggt tccgtgcaag ccggcgtaaa agccgatccc
   128641 gatatccaac ccggatcgtg cttcttcccc gagcatttta atgagccggc cgtgaaggac
   128701 ctgatggccg tagaggtgga cgcagtcacg ggagttccgt acttcaaatc cacgcgtgta
   128761 aagattgaaa aggttggtgc gtaagagtaa gctcgctcgt ctttttgatg tcgacagagg
   128821 tggtgagaat gagtgtcggg gccttgacca agaaaattct tcaggcggcg ttgttctacg
   128881 aaatttggga cgccatgaag gtgaccttca agcatatgtt tcatcgcccc atcaccttcc
   128941 aatacccgcg cgaacaacgc accatccccg acggtcaccg cggcgcatta gggctgcttc
   129001 gttatggaga cggccaggac cgctgcgtgg gatgtgacct ttgcgaagcg gcatgtccgt
   129061 cccgttgtat caaagtcatc agccaggaag acccagaacg tcccttgcag cgctttgcca
   129121 gcgagttcta catcgacatc accaagtgcg tgttttgcgg ttattgtgtc gaggcatgcc
   129181 ctgtcaacgc gctcgcgatg accaagatgt acgaatactc gacccacgac aagcgcaccc
   129241 tcctcttcga caagaaaagg ctctacgagg tcggcgaacg tcacctcgag gacgccaaaa
   129301 aatatctgta cgcccataac caagaaaaga attcggacga gagccgggaa tatcgttatt
   129361 actttccgca gtcggttctc aaacccacac agtcacaacc caaacatttg agctgatcgt
   129421 accgttatgg ccttcgtgtt tttcgcgtat ttcgcagtgg taagtattgt cgccggcatt
   129481 ctgaccgtcg cgctgaagaa tccggtgcaa tgcggtctgg cgctcctggc cctgctcctt
   129541 cacgtctcag gcctcttcgt catgctgaac gccgaatttt tgtgggcggt ccaagtgatc
   129601 gtctacgccg gggccatttt ggtcctctat ttgttcgtcc ttatgttgct gaacctcaaa
   129661 accgacgacc gctatttcca cgccaaggct ccttatcttc ttgcaccagc ggccatcggt
   129721 ttggcctacc tcctgttctt gttggtgaga tccccgttcg gcggctccag gggagatgcc
   129781 tcaccggcgg cgatcttgct gaacggagat gctcatgcca tcggcatcaa gatgttcagc
   129841 gagtatttgc tgcaatttga aatcatcggg gtgttcctga caggagccat cgtcggcgcg
   129901 atcgtcctcg caaagacccc caagtcgatc gaaacgaggc gcgacgcatg attcccttgt
   129961 ccgcctatgt ggctgtaagt gccgtgttgt ttgcgacggg gttgctcggc gtcctgatcc
   130021 gtcgcaattt tattattgtg ctgatgtcgg tcgaaatcat gctgaacgcg gccaccatca
   130081 acctggtcgc tttctcccac tacctggagg ccatgcaggg acaaatcgtg gccctcttca
   130141 ttatcgccat tgcagcgggg gaagcggcta tcgggctcgc catcatcatc gtggtgttcc
   130201 gcggcaagat ttcaaccaac gtcgacgaaa tgaatttgtt gaagtggtaa cgtgtccgac
   130261 tctattgatc tgatcgtcaa actgattcct ctgttcccgc tgatggccgt cattgtgaac
   130321 ggcctcttcg ggcatcggta ttctcacgac ctagctcacc ggttcgcgtg gggatcggtc
   130381 ctcatgtctt tcctctgtgt gctggccgtc ttcacggaga ccctgcgtac aggcgcggct
   130441 cgtgaagtgg tcgtctacaa atggattttc ggcggcgacc tcacaatcaa tctcgcctat
   130501 ttgatcgatc ctttgacctg catcatgctg ctggtcatca ccggagtggg attcctgatc
   130561 cacgtctact ccgtgggata catgcacgga gaatccggct tcacgcgatt ctttgtgtac
   130621 atgaacctct ttatggtttc gatgctcctg ttggtgatgg gcaataacta cgttgtcctc
   130681 tttatcggct gggaaggcgt cggactctgc tcctatctgt tgatcgggta ctactacgac
   130741 aaggtgtccg ccgccaaagc ggccaccaaa gcctttgtgg tcaatcgaat cggtgatgcc
   130801 ggatttctcc tcgcaatctt cctggtgttc gtgaacttcc gaaccctcga ctacacccag
   130861 gtgatgcaga acgccgcaca gctctcgcct gaaatggcca ctgcgatcgc actctgcctc
   130921 ttaatcggcg cggtcggcaa atcggcgcag ctcccgctct acacctggct accagacgcc
   130981 atggaagggc cgaccccggt cagtgcgctc atccatgcgg ccaccatggt cacggcaggc
   131041 gtctatatga tcgttcgcaa tcatgccatt ttcgatctgt cgcccaccgc catgacgacc
   131101 gttgcctgga tcggcggact caccgcgctc ttcgccgcta caatcggcct ggtacagacc
   131161 gacatcaagc gcgttctggc ctattccacc gtcagccagc tcggatacat gttcctcggt
   131221 tgtggtctcg gagcgtatac ggccgcggtc ttccatctga tgacccatgc gttctttaaa
   131281 gcgctcttgt tcttgtcggc cgggtccgtc atccacgcat tgtccggcga gcaggatatc
   131341 cgcaaaatgg gcgcgttaaa gtcgaagatc ccctggaccc atacactctt cctgatcggc
   131401 acgatagcga ttgccggaat tccgcccttg gcgggcttct ggagcaaaga cgaaatcatg
   131461 ggccatgcct tcgtgcatca tcactatgtg ctctacggca tggcagcggt cggcgcattc
   131521 ctgacctcgt tctatatgtt ccgcctgacc tatctcacgt tctatggcac ctcgcggctg
   131581 gatcaccaca ccgccgaaca cgtgcacgaa tcccccatgg tgatgatcgg gcccctcgtc
   131641 gtgctggcca ccctctcagt cattggcggg ttccccggtg ttccgcctga aaacggctgg
   131701 ttccaccact tcctgcactc agtcgcaggt gcggccgggg aggagcacgc caccgcgccc
   131761 gcgctgatgc tcgggctgat gggcacggcc accgtcattg cgctcctggg ctggggactg
   131821 gcgcattact tttacagcgg gccttcctcc gcagcagatg cgctcgccgc acggatgccg
   131881 ggcgtgtata caaccctgct gaacaagtac tttgtcgacg aactgtacga ccggctgttc
   131941 gtggaaccga ccaagcagat cgggagactt tgcgactggt ttgaccggac catcatcgac
   132001 ggcctcgtcc gatctatcga tcgggggaca gacgccagtt cggcggccat cacctggacc
   132061 gaaaaacatg tggtctatgc cggcttgaac atcatcggct atgcgaacca ccgcttggcc
   132121 cgctcgtggc ggcggcttca gaccggcatg gtgcatcagt acgcggctat cattgtggcg
   132181 ggacttttta ttctcgtgca cctcattctt ttgatttgga ccggaagcgg ttccaacggc
   132241 tatttatcac gctgacgcag cagaatttct ggacacacga tgcttgaaga gttcagtttt
   132301 ggcttcccga tcctctcgta tctgatcttc ctcccgctcg ccggcgcggc ggtgctctgg
   132361 ttgatcgaag acgaagatct cctcaagacg acgacattgg gtatcaccct ggtggaactg
   132421 gccctggcct gcctggtgct ggtgcgcttc gtgccggatt ccgctgcgat gcaattcgcc
   132481 gagcatgcgc gctggatgcc ggcgctcggc atcggctacc atctcgccgt cgacggcatc
   132541 agcgtgctct ttgtagggct gactgcgttt ctcaccgtgc tggtcgtcat ctattcctgg
   132601 gataccgtac gcaatcaggt ccgcctgtac ttcatggcgc tcttggccct agagaccacc
   132661 accatgggta tctttgcctc gatcgacctg attctgttct tcgtcttctg ggaactcatg
   132721 ctcatcccga gctacttcct gatcaaattg tggggcggag gagcagagcg ccattacgca
   132781 gccctcaagt atgtgctcta cacactcctg ggcagcgtct tcatgctggt cggcatcgcc
   132841 ttgctggaca tcaactatca ccagtgggcg gtgactcacc acctcgacca cgtctactca
   132901 ttcgacctgc tggagttact ctcggtcccg atcccgctct cccagcagat tttgatcttc
   132961 tggctgatgt tcatgggatt cgccttcaaa gcgccggtgt tcccatttca cacctggctc
   133021 cctgatgccc tcgttgaagg acccatcggt atggccgtca tgttagccgg gatgaaattg
   133081 ggtacctatg gcttccttcg ttttaccttc ccgttacttc ccgatgcatc gaaaagcgaa
   133141 ggcgtcgtct cgatcgtcat ggtcctggcc ctcgcggcaa ttctgtacgg agcggtggtg
   133201 gcgctcattc aatcagactt caagcgcctc ctcgcattca gcagcatcag ccaccttggg
   133261 tttgtggtgg tcgggctctt tgctctgaat ttccagggct tgcaggggag cctcctgacc
   133321 atgatcaacc tcggtttcag caccgccggc ctcttcttca tggcaggctt cttatccacc
   133381 agacaacaga gttcccaatt gtcctccttc ggaggattcg cgaaacaggt accgctgctg
   133441 gcctcgttcc tcctgttgat cggtatggcc tccatcggac tgcctgggac caatggtttt
   133501 gtgggtgagt ttctgatcct cctcggcgcc ttcaaagcca aatggtggta tggcgccgtg
   133561 gcggtcaccg gcgtcatctt cggagcggcc tatttcttgt ggtattacga gcgagccatg
   133621 ctcggcccgc tgagcaagaa tatctccgcc acgatcaagg acctgcacat gcgggaaatc
   133681 accatcgcac tctcactctc ggtgatgatc ttgtggatcg gcctgtaccc gtcacccttc
   133741 ctgagaatga tgaatggatc gattcaagcc ctcgtcgatc gtttggatcg tgccaaagtg
   133801 gcctcggtag atactgtcat ccacgtgccg gctaagtagg accatggctg aatacacgct
   133861 gctcattatt ttgttcgcgc cgtttgtcgg ggccctggcc ctgatcttcg tctccaaccg
   133921 ccagctctcg ctggtccgcg gcattgcagc cggctcggcc tttgtctcat tgatcgcttc
   133981 agtctatctt ttctatgcct acgatccgtt gaaaggcggc taccaattta tccagcggat
   134041 tgaatggtca cgacagctgg ggatctccct tcatctcggg gtggatggaa tcggcacccc
   134101 gctggtcctg gcctccggca tcctcctctt cgccggcatt ttcgtctcct ggcacatcaa
   134161 agaccgggtc aaagagtttt acatctggat tctgattctt gccgcggcga ccatcggcgt
   134221 attcatgtcc ttggatttgt tcttcctgta tttcttctac gaaatgtccg tgatccccat
   134281 gtatctcctc ttgggcatgt gggggagcca cacgaaaaag tatctcgaga tgacggacgc
   134341 cgaaggcctc aagcagcggg attccgtcgg ctttatcttc aatttcgccg cgaacagcaa
   134401 agaatatgcg gccatgaagt tggtcctgtt tctgtctgcc ttcgccgtcg cggcattgat
   134461 gggcattctc ctgatttaca agttctcagg actcaacacc ttcgatattc tggtgttgcg
   134521 cgagcaggcc catttctcgg ggcctctggc cacgctgatc tggctgttga tctttttcgg
   134581 ctttgcgtcg atcgctccga tctggccgct gcactcctgg tctcccgtcg gccacgcggc
   134641 cgccccggcg gcaaccagca tgttgcacgc aggcgtgctg atgaaattgg gacatttctc
   134701 gattatccgg gtcgccttcg aaatcctgcc cgagaccacc cgcgaactca tgcccattgc
   134761 cgcggtgctg tgcatcttca gcatcatcta cggcgggctc gtcgcctatt acgcaaagga
   134821 caccaaatac gtcattggct attccagctc cagccatatg ggctacgtgt tcctgggaat
   134881 ggccgccctc gactacatca gcctgagcgg ggccgtcatc tatatgttcg ctcacgctat
   134941 ggccaccggg atgctcttcg ccatggccgg ctgggtctac gatcaaaccc acacccgcga
   135001 cattccctcg ctgggaggcc tctccaaccg gatgccattt atttccgccg ccttcgtcat
   135061 cggctgcatg gcctcgatcg gaatgcccgg caccgtgaac tttatcgctg aagtcatgat
   135121 catcgtcggc agttggaaca aatatccctt ccaggtcgtc gttgccgtcc tcggtatcgt
   135181 gctgaccatg gcctatctct tcaagatgat gcgcggtctg ttctatggat ccatggcgga
   135241 gaaatacagc cattcacatg atgccgtgac cgtcgttgat cgtatgccgc tgctgatcat
   135301 gatcaccgtc agcatcagtt ttggaatttt cccggggcac ctctactccg tcgtccgatc
   135361 cggggttgat cccttgatcg ctcgtatcac caaggtcgtc ccggttgcgg agcaccctat
   135421 gaaccttcca catgccacct cgccaaccgt acccacatcc gcttcccgtg aagcacttgc
   135481 gaaggtgacc ccgcgatgac tttttccctt accctgtcag tcagcgatct cctgttgctg
   135541 ctaccggagc tgttcctcac tgcctggctg tgcgtcatcc tgatcgtcga tttttcgttc
   135601 aagcggatcg tccaggaaca gctcgcctac ctgagcattc ttggattagt gatcacactg
   135661 gcctgcctgg cctggtttga cgtgaccggc atcaccggca ccctgttcgg caagatgttc
   135721 gtgctggacc ggatggcgat cttcttcaaa gtgatgatcc tcctcgccac gatcctggtc
   135781 attctcttgt cgatcgatta tgtgcaccgc tttgcgttct tccgtggcga atactatttc
   135841 cttgtcgtga tgtcggcatt aggcatgatg ttcatgacct ccgccaacga tttgctgtcg
   135901 ctcttcgtca ccctggagtt cgcgacattc ggcttttatg tcctggtgtc ctatctccgt
   135961 gacgatatgg catccaacga ggccggtctc aagttcttca tcctgggagt ttttgcggcg
   136021 ggcttgctcg cctatggcat cagcctggtc tatggcgaaa caggcaagct cgtcttctca
   136081 gacatgactg gcgcagcccc gactaccgga ctcgtcatcg gcttcctgct catttttgcg
   136141 gccttgggat tcaagatcgg cgcggtccca ttccattcat ggatccccga cacataccac
   136201 ggcgcaccga cgccggtcac cgccttcctg tcgatcgcac ccaaggtagc cgcctttgcg
   136261 attttgctgc gcctcttctt agtggcgctg gcgaccttca aaccggcctg ggcattgctc
   136321 atcgtggcaa cctccatcct ctcgatgacc tacggcaaca tcgtggcgat cgcacaacga
   136381 aatgtgaaac gtctgttagc ctattccggt atcgcacaag tcggcaacgt gctgattggt
   136441 ctggcggcgg ggaccaagat ggggaccgat tccatcttgt tctacctcct gacctatctc
   136501 tttgcgaacc tcggagcctt tgccgttatc atggcgatca gcaatgcagt gggcagcgat
   136561 gaaatcgatg actacagagg actcaaccgg cgctcgccgt ttctggcctt tgctatgctg
   136621 attttcctgt tgtcgctggc cggagttccc cccctcgccg gtttcatcgg caaattgtat
   136681 atttttgtcg ccgcgatcaa ggaggggctc tatatcctga tcacggtcgg cctcatcaac
   136741 atcgtcgtgt cgatgtacta ctacctgatc gtcgtcaaga agatgtacat caacgaaccg
   136801 ctcgattctt ctcccatcaa gacgacgggt ccgttacggg cggtcatcta tgtcggattg
   136861 gccggcacac tcgtgatcgg tatctatcca cagccgttcc tcgactgggc cgtagccgcc
   136921 acgctgatgt tttccaatct tctaggccct gccgcttcca ttcccgtacc agtcgtgccg
   136981 ttcggcgggt aagggccagc tccgatccgc cttacgaatt caatcaaatc ccttcgcgta
   137041 tcaatagctt ctgctacaat acaatcgcct gatctctgag cggttcgccg aggctctgca
   137101 gccttgctcg gacgcttctc aatacctccg cctggttctc gcatgagtat ggacgaaccg
   137161 aaaccccaac aggccgtcaa cacagacgtg gagtccgtcc ttcctaccct tccctggagc
   137221 gcacgtattc tttcgcgtct ttctattctt gggccgttgg tcctggcatt tcagaagcgc
   137281 tccattgaac aacgcgtact cgctggcttc ggccttgtgt ttgccggcat cctggtcatt
   137341 tccgccattt cgtatcgcaa tatgacggtg ctcatccgaa acggtcatca cgaccaacgg
   137401 agtcacgagt tcatccaatc cttggccgcg accggggagg ccatcgacga tgccgaaaac
   137461 ggccaccgac gattcctggt caccggggac gaaagttacc tctccgccta caacaccctg
   137521 caagaacgcg caccggaata cctcggctac ctgcgggaac tcaccgagcc gggcagcatt
   137581 cagcgcgcac gcgtcaccca actggaacag ttgatcaccc aacaactgcg ggaagagcga
   137641 gccgccatcg acctgcgaaa ggagacgggg ttcgagtctg tccggacatt ggccctggcc
   137701 ggtgtcgcca aaaccgcgct cgattccgtc cgtcgactgc aagcgcagat ggagcaggac
   137761 gaaaccaagg ctctcgcgca acgggtcatc gaatccacca cgaccacgcg ctcgagcatc
   137821 atcctcctga tcatcggcgc ccttctgctg ttcgtactgc tggctgccgt gtactatctg
   137881 attcggcatg acattacggc acggcggcgg attgccgatg aactccagcg ccggggcgaa
   137941 ctgctggagg ccgccaataa agagctggag gcgttcagct attccgtctc ccacgactta
   138001 cgagccccgt tacggcatat tgacggctac gcctcgctgc tggccaaggc cgcctccatc
   138061 tcgcttgacg acaaagcgaa gcggtactta cagacgatct ctgaatccgc caccaggatg
   138121 ggacaactca tcgacgatct gttagtgttt tcccggatgg ggcgacagga aatgctgcgc
   138181 gggacggtca acctgaacca gttgatcgcc tccgtgctgc acgatctgcg acatgacttg
   138241 caagatcgga caatctcgtg gacaatcgct caactccctg aagtcacggg ggatgcagcc
   138301 atgttacgac aggtctttat gaatctcgtg gccaacgcga tcaagtttac cggtacgcag
   138361 ccgcaggcta cgatcgagat cggaagccgt gacaacgggc aggaggaagc ggtgttattc
   138421 gtacgcgaca atggggtagg atttgatatg cgctacgcga acaagctgtt cggcgtcttt
   138481 cagcggctcc accgagcgga tgagttcgag gggacgggta tcggactggc caatgtcaga
   138541 cgcattattc accggcatgg cgggaagacc tgggcagaag gtgcgctcgg cgaaggcgcg
   138601 accttttacg tcagcctccc gttggcgagg acatcggcat gaccctgacc aaacccatat
   138661 tactggcgga agacaatcca cgcgatgccg aactcgctct ggcggcgatg gaagaacatc
   138721 atcttgcgga taaggtcata ctctgtcacg acggcgcgga agtattggat tacctctact
   138781 gccgtggcca cttcaaagcc cggctccagg gcaatcccgc ggtggtactc ctcgacttga
   138841 agatgcccaa agtcgacggc ctcgaggtac tgcgcaccat caagagcgac gctgccctga
   138901 aacccatccc cgtggtgatg ctgacatcgt ccagggaaga gcgggacctg gttgaaagtt
   138961 atgctttggg cgccaacgcc tatgtggtca agcctgtcga gtttcatcag tttctcaaag
   139021 ccgtcaaaga gctcggtgtg ttctggggaa tgatcaacga accgcctcct gagggggcga
   139081 gccgcgtaac cagccgatga cctaaccccc gttcaggact ctgttcccga tacacacgat
   139141 gactcagccg ttgcgcttca tccatcttga gagcaacccc gccgacgcgg cgctgattgc
   139201 cacgaccctc cgtgacgccg gcattctatg ccagctgaaa caggcgcaga cacgtgagga
   139261 atttctggcc gcacttcgcc aggaggggtt cagtctcatc ctagccgata ccgccgtccc
   139321 cggcttcgac ggagccaccg ccctctccct cgcgcgcacc ctgcatcccg acgtgccgtt
   139381 tctgtttgtc tccgatacac agggagaaga gtttgccatc gacatgatgc agcacggtgc
   139441 caccgattac atttccaagc agcgcctggg gcgtctcgtt ccctccatca agcgaacctt
   139501 gcgggaactt gacgagcggc tggaacggag acgggcggag gacgccctgc gggtgagcga
   139561 aaagcagttt cgtcaggccc agaagatgga agccgtcgga cgtctcgccg gaggacttgc
   139621 ccatgatttc aacaacctgt tgacggtcat catgggacat agccaggtgc tcttgggaga
   139681 actgccctca ggcaatccca tccgcgccaa gatcgaagag atgcaaaagg ccggggagcg
   139741 agccgcgagt ttgattcgcc aactcatggc cttcagccgg aagcagccgc tggaaccgaa
   139801 aatcctgccc ttgaattcgg tcgtcggcaa tgtggaaagc atgctccggc gactcatcgg
   139861 tgaagacatt caactcgtga tccgccctga tccttttaac ggccatgtga aggcagaccc
   139921 cggacaactg gaacaggttc tcatgaatct ggtcgtgaat gcccgggatg ccatgcccaa
   139981 cggcggccta ctggcgatcg aaacctcgca aacggaactg gcacgaaccc cgatgcatca
   140041 tctccacccg ctgccactgg gacagtatgt gaaactcact gtcaccgata ccggattcgg
   140101 tatgaatgcc gacgtgctcg cccatctatt cgaaccgttt tttacgacga aggaagaaca
   140161 caagggtacc ggactggggc tgtcgacggt cttcggcatc gtgaccacct gcggaggcgg
   140221 gatcgatgtg tggagtcaag tcgggcatgg caccaccttt gacctgtatt tcccgcgcgt
   140281 caacccgcaa gcctctactg cggatgccac cgcaccgcaa ggccgcctgc gtcagggatc
   140341 ggaaaccatt cttctcgtcg aggacgatag cggggtacgc aacctggtcc gtcacgaact
   140401 ggtcaaaacc ggctatcagg tgatcgaggc caagaatggc gtcgaggcct gtctcacggc
   140461 cacacagcag agttaccatg tagaccttct gctcaccgac gtggtgatgc cggggatgaa
   140521 cggtcgcgaa ctggcacaac atctgtccgt catcaagccg aacctcaggg tgctgttcat
   140581 gtcgggctat ttggacgata tctccgtgaa cagcggcatg gacccgcatc gaacaacgtt
   140641 cctccagaag cctttcaccc ccgacctcct cttgcgcacg gtacgcgcat tgctcgattc
   140701 ctccgcttcc gcgacgggaa gcgcaccgca ccattcccct cccgaccagg caccccgcac
   140761 cgcacgagcc tcctgacggc tcgtctgcgc acaccgcaga caccaccgga aatttgaccc
   140821 ttttgcgcat cctttttata ctgaggtcgc gcgagatcgg gaaagcacat tcacagaggt
   140881 tgacaggagg aaagagagac gctcgtcatg cgtggccgct gcctgaagga ccgacgatcc
   140941 gttcaccggt tgaagctcct ctgcgggtgc ttgctcgcgc tctgcctcag tagcggaccc
   141001 caagccggtg cggcgcttcc cgccggggtg ccatgggagc gcttgaccac aggtatgcag
   141061 gtggcgctct ggagccccat tgaggcctgc ccccaggttc cgtccctgct catgctgcac
   141121 atcgatccgg aacggttccg gttctccatc tatcagtatc gcgatgaagg tctccgcgcc
   141181 ccgctctcga tccacgactg gcagcaacgg accgatgcgt acgtcttatt caatgccggc
   141241 ctatttcgag aggactactc gtacttgggt gtcttattga aagaagggcg gtcactcggc
   141301 acgaagaaac atcactcatg gcagggacta ttcgcagcgg aaccgaccga cgggaggctc
   141361 cggaaggccc gtgtgctgga ccttgccttc gacggattta cagaagaaac ccctccctac
   141421 cgggaggccg cacaatcgct gatgctgttc gaccggacgg gaaaactccg ggtccgagac
   141481 agcgggaaac gcgcgtttca aaccgtggtg gctgaagaag gggaaggcgc aattcttgtg
   141541 atcaaaacgg tggatatcgt ctcgctccac cacctcgccg actgtctcca tcgacagatc
   141601 ccgtcgatcc aacaggccat ggctatggac ggaggcgcct cttcggatgt gatcgccagc
   141661 ccggatcttc tccatgccgc ccaggagacc acgtcgcaag ccacctggcg atcactcctg
   141721 gcgggcaata tcggcgtgca cattccgctg ccgactgtca tcggcatcag cccccggacg
   141781 cacccgcgca ccatgcccgc cccggacgcc tcaacatcat cacacagccg atgaccaacg
   141841 ccgatctacc gtccaggcga gacgggaaag ccggcctcag tccaggcatt catgctgccc
   141901 acgacattgt agacgtgcgt atagcccaaa tcggccaacg tctcggcggc aatgttgctg
   141961 cgatggccgg actggcaata cacgacgata tggtcgttca aggccgcacc gatctcccga
   142021 tgccgcgcct tcatctcgcg aaagtcgata ttccgatcgg tccccggaat catgccggac
   142081 gcatgctcct cgggcgaacg cacatcgacc aacaggaacc ccttcgctcc ggtcgatggt
   142141 gccttgagca gaccggctcg cagttgctga accgtcaagg tatacgaatg atatgcgaag
   142201 gtgggctgcg gccactcgaa gaggagaccg cacagcacac ccacgcagag aagaccagtt
   142261 agatatctca tcgtcgccgc tcctttgctc atcccgagaa ggttgacgaa cgtcctatga
   142321 taggagcctt atgaaaagct ggtcaagagt agttgccgtt tccttgctcc tggggacgtc
   142381 cggctgcggg gaaggagcca tcatggtcaa agaagtcgac aacggcggcg tagtggtcta
   142441 cccctatcgc gccgaacagg gcaacttact ctcgtccttc cggaaggaag cggtgcggct
   142501 catggagcag aaatgcccag ggggatggat gacagtgcgc gaaggagaaa caaaaggacg
   142561 gctgcgccaa gccagtccca tcgcgggcgc gaacgatatc gtcgaagaac gccgttgggg
   142621 cattcagttc cagtgcaaat gagctaggaa gaaatgttgc gcccctgcat gagatcatcg
   142681 atcgtcagga ggctcacgac cttcactccg gtggcctcca cgcgggcacg gccgtcctgc
   142741 tctttccgat cgacgatgac cactgcatcg gtcaccttca accccgcctc tcgcgcggcc
   142801 gcgatggcct tcagcacgga ccccccgctg gttaacacat catcgacgat gagggcccga
   142861 tctcccgcct gcacgacacc ttcaaccaat ctgcccaacc catggtcctt cggctgcttc
   142921 cggacaaaaa atgtccgcca ctcccgacga ggcgtcgcca aataagcgaa gtctgaaatc
   142981 gtcgtcgcaa tcgagatcgc tccgatctcc aaccctccca gacaatcgat gtcgagcccc
   143041 ttggtcactt cccacgcccg ttgcgccatc aagtgacgcg cgtgaggaaa ggccatcaag
   143101 gtgcggcaat cgacataaaa cgggctcgtc agacccgagg cgagtttgaa gccgcccttc
   143161 ggatcccact tgaacgactg agtcttgtga aacgcggcga tcagatcatc ctgcagcata
   143221 accgtttttc ccatgcggta atgaaatctt acgtatccac atctaatgag ttcaacgtca
   143281 cgaaaacatg aatatagatg gaccacgccc acccttcagg gtacgatcaa atggccctat
   143341 tccagagagc cgtccctgcg cggcagacac ctagcaggat cctctcatcg cgggcgggca
   143401 cgtggtcgtc ggccgcttcg gtggtgcggt ttgttcgatc gcggaaggag gctccgtttc
   143461 ttcggcggcc ggccgagggc ttgactcaac ggaatgatcc gaaggactcg cgcagcctga
   143521 ccacatcccg atcacgctca gaagcagcaa ccacttcaac acaccaccgc ctcgcgggga
   143581 accattgacg ggctcgatgt cagggcattc tgacatagcc cggcggcaaa gtgaagcaag
   143641 ggtagatgcg ccacacgatg tgtcgacttc tttcgcgact cggccgggat ttcctgatcg
   143701 tgacttgcct gtggacgatc tctgcgacgc acgcagccga acaaccggcc gctcccgccg
   143761 ctgtgctcgt gaagggcttt tcattcagcg ggaacaccgt gatctcccaa gccgagctcg
   143821 aagccctcac gcagccctat gtcgaacagg cactgaccct ccccgatctc gaacgcatcg
   143881 ccgccgccgt cgccgacctc tacaaacagc gggggtatac cctcgcaagc gcgtacattc
   143941 cccagcagcg cattcggttc gagatggtcg agatcagcgt gctcgaaggc cgtctcggcg
   144001 acatctcggt ctccgggaac cggttctact cgacggaatt cattcgcagc ggattcgccg
   144061 gcgtgatgga agcacgggtg atcaggaata tcgacctgga gcgagccctg ttgttgctga
   144121 acgactatcc tgacctcaag gtctccgcgg cgctcgaacc gggggaattc acgggcagca
   144181 ccaatgtaca ggccagcgtt gaggacaagc ggccgattca cgccacgctc gattacaaca
   144241 actacggctt caacaacatt tcgagaaacc ggttcggagc cggcattgag gtcggcaacg
   144301 cgctgagaga aggggccatt ctgacattca acggaatcgt gggggaccat cccgaacggc
   144361 tgcaatttat caccggcggg tacgccctgc cggtgggcgt gcacggcacg aaggtggtgc
   144421 tttcaggatc cacaggccgg ttcgacgtgg gcgccgagct ggccgctctc cagattcgcg
   144481 ggaaaataca cacctacgac ctgtcgatca cacatccggt ggtgaaaacg cgctggcaat
   144541 ccctcgtact cgataccgga ttcgccgcca aagataatcg actgttcatc aacggcgcgc
   144601 tcaccggtca tgacaccgtc agaatggcca agctcggaat gaactacgat cgagtggacc
   144661 tctacggccg gacctatctg tcggcctacg gttttcatgg cttgggcgaa atcctgggcg
   144721 gaatggacga tcacgcggcc atcagcacca ggccgggtgc cgacaatcgg ttcctcaaag
   144781 gcacgctcgc cgtggggcgt attcagagcc tgggtcacga cgcgctgttg atcctcaaag
   144841 gaaccggaca gatcacgagc ggccccatgg tcgtcatcga acagatgtta ctgggcgggc
   144901 cggattcggt ccggggctat caactcgggg aacgattcgt agacgagggc tacgcggtaa
   144961 ccgctgaaac acggataccg ctcctgccct ccatgctccc ctcgacgcag gcggcgctgt
   145021 tcttcgatca cggcgccggt cacctgcgaa accctcaacc gggagagcgg agatacagca
   145081 gtctgagcgg agccggtgtc ggcctgcaga ctgaactccc gtactattcg atacacctgc
   145141 gagcagacct ggggtttccg gttggtccaa aaccgagcgg gggaaccatt gccggggacc
   145201 gtagcccgac gatgtatctc caggcggcga ccaggttctg atgtacgatt cacccattca
   145261 taagacggca cgggcgccga aaacgggccc cgcttcattt gccttcagcg agccactgct
   145321 gcggagacat cgcatcctct gcgggttgct cgccgcagtc caactactga ttccctccct
   145381 caccctcgcc ctccccacag acggccgggt cgc
//
LOCUS       DAORRQ010000004       116986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_4_length_116986_cov_69.576443, whole genome shotgun sequence.
ACCESSION   DAORRQ010000004 DAORRQ010000000
VERSION     DAORRQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 116986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 116986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..116986
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4_length_116986_cov_69.576443"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PK224_02270"
     CDS             <1..674
                     /locus_tag="PK224_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRB14592.1"
                     /translation="ASGYQFVSQEEVFRLAEPVPDGATTKRLVEIAARRGMTIVAGLP
                     ERDGRHCFNSAVVVGPRGFIGCYRKTHLFFEETLWFTPGDSGFHVWDIGMAKVGVMIC
                     FDWYYPESARTLALQGADIIAHPSNLVLPHCPDSMVTRCLENRVFSVTANRIGSEARG
                     GKDRLTFIGMSEVVSPRGRILHRAPRETEDLTIVEIDPAEARIKALNAYNDLLRDRRP
                     ALYGD"
     gene            739..1299
                     /locus_tag="PK224_02275"
     CDS             739..1299
                     /locus_tag="PK224_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRB14593.1"
                     /translation="MSIPLGKGILLVAAPALNDPNFRQTVVLLCEHGPEGALGVIVNR
                     PTAMSISEALPQVPILEGQPHVLYSGGPVQTNQVMMLYRINQTPENSHQVFDGVCLGG
                     DLEIMERILMEQPGQESFRAYLGYSGWGPGQLESEMQVGSWITLPADPSIVFEKEPTR
                     IWSDIFLSLDDTSRHYADMPFDPSSN"
     gene            complement(1615..4416)
                     /gene="uvrA"
                     /locus_tag="PK224_02280"
     CDS             complement(1615..4416)
                     /gene="uvrA"
                     /locus_tag="PK224_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRB14594.1"
                     /translation="MAHRSPPDCTTNDLIVEGARQNNLKNISLRIPHNRVTAITGLSG
                     SGKSSLAFDTLFAEGQWRYVESLSTYARMFLDKVNRPDVDRITNIRPAIAIEQKNPIR
                     TARSTVGTATELADLLRLLFAKIGKPVCPDCKQEARGYHPGSVAEELLARFPDARVMV
                     LFPLKDLGPGHDRSLLDSLLKRGFTRLRCGEELLDLHEQAVLPVTRESGIQVVLDRLV
                     LRPDNRHRLIEAIETAFQEAEGTCQVLVIGQGLRTYSTHFRCQGCGRTFEPLRPLLFS
                     FNHPLGACPECKGFGNILQYDKDLVIPDRSKSLANGVIEPWSKPGSDWWQKQILLAMK
                     KQGVDLTTPFQELPEEVQQLIWEGSDQVEGVRQYFDYLETKRYKLHVRVLLSRYRSPA
                     TCPTCHGSRLKPAARLVKLAGQDIVGIGELTIEAAAAWFERLALPAFDAEVAKDILRQ
                     LHAKLNFLLRVGLSYLTLSRQTKTLSGGEAQRIALANQLGSRLVGTLYVLDEPTIGLH
                     ARDTDTLAGILRDLANHGNTVVVVEHDPSMIQAADHIVEMGPGSGEQGGHIVCAAPRE
                     QFLADQASLTARYLRGENRIPLPKIRRSGNGKVLSIAGAAEHNLKNLVVRIPLHMLVC
                     VTGVSGSGKSTLIEKTLYRAAARAFRIESLPMGKFQAIKGLEHVKGVRLIDQQPIGRT
                     PRSNPITYLKAFDEVRTLFASEREALRRGLTPGHFSFNSADGRCERCEGNGYEKLEMY
                     FFEDIYATCEQCDGKRFKPEVLSIRYRGKTIHDVLNLTVTDAQAFFSGSPKLTEKLYL
                     LSSIGLGYLRLGQAATTLSGGEAQRLKIAAELKDPSAHNLLYILDEPTTGLHLDDIKK
                     LLTVLHKLVDAGNTLIVVEHNLDVIKTADWVIDLGPEGGEAGGQIVAEGRPEQVAKVT
                     QSHTGKFLAKVLGGAPRTS"
     gene            4560..5339
                     /locus_tag="PK224_02285"
     CDS             4560..5339
                     /locus_tag="PK224_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HRB14595.1"
                     /translation="MVDRSAYLARIGYQGSVVPSMETLRGLHLAHVLTVPFENLDIHL
                     GRPISLDPSDLFRKIVISRRGGYCFELNGLFALLLEELGFVVTRLAARVLYGTEDVRP
                     RSHQVLLVHLDGERWLVDVGFGGQGLREPFLLVAGLEHRQGPDQFRLATDERGEHVLQ
                     CHIEKAWTNLYSFTLDPWLPVDYQFANYYHSHAPESPFVQRWMCTMPTLNGRKIFTEK
                     LLKVRGPEGVHKLHVTSEVECKQLLQEHFGLIINDHLRFLV"
     gene            5379..6374
                     /locus_tag="PK224_02290"
     CDS             5379..6374
                     /locus_tag="PK224_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRB14596.1"
                     /translation="MAAQKGRRPKTYSQADRLARMLRALASRSMTVKDLAEAFEVTKR
                     QVYRDLDRIHEEGHPLEQSDGDGEKTWHLPLGYKGLPPITVSPYELMALHFAKRHLDY
                     LTGTPLVDDLDSLIKKVEGGLSHRVFNHLARINQVFLPRSGPVRDYAGRRVVLKQLQK
                     ALLLQNIVVLRHRRPDYDEPAEHRVDPYVLLLYQFGLYLVGYSYRAEALRMFAVERIV
                     SVEVLEERFSLPEEVQVGKIYDNLFGLVDEPTQTVRIQFDPDVAYLVKERRWHPSQQN
                     EIQKDGSVIATFTAGGMDELASWVLSWGPAARVLEPQALVEAVKTHLAHALERYR"
     gene            6653..7924
                     /locus_tag="PK224_02295"
     CDS             6653..7924
                     /locus_tag="PK224_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14597.1"
                     /translation="MLTRRNLFKFAATLGLGTFAPWLDRSTRGAAMELRPGRNPPSDS
                     FFSQPERSLQEMESLENLKDDLDCVEVVAYVASARSMTCSAEVNLIRGSSHCPFCGEA
                     GFRVGTESFTCKWCEAEGSAIEFYARAEGLPHEVAAVRLEALLNDGLLKGRRPENAHA
                     YRMLEEAARFYHALLCERPEGAVARECLVEQGIAPSTIERFRLGYAPHDPDDLLSRHL
                     VAEGYVIPKLLQTVRVNEAGEEKLIDRYGGGHLLIPICDQAGRSWGFMKRRLTSRQDS
                     LQAGWTQDTLAVSERRLRRLIFPHPAWPQEFNRHQRVLITETPWEVVALHNAGIENAV
                     YLFDCATDPVRLRTLLSLGQDLICCVDPKGRRSVMALRLIEVLEREGRPLRVMSVPSK
                     GGLMELLQASGPQAVRKAMSQAIPFHQWLEA"
     gene            7946..8185
                     /locus_tag="PK224_02300"
     CDS             7946..8185
                     /locus_tag="PK224_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14598.1"
                     /translation="MEWLLPLILQIVIPADSLMQVTLNKSLAQVIQEQISCRKEFDEA
                     RPEFERVNSIDDAIARLNAIDRLRRSVLLRCGVRP"
     gene            8246..9019
                     /locus_tag="PK224_02305"
     CDS             8246..9019
                     /locus_tag="PK224_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14599.1"
                     /translation="MLQAILHGKIGMLFEKGAEGESFKERFRTLEDFLTAAVFERLRY
                     LPANLFWKVLSEAAIPVGSLPMHVGNLRESEFWPTWDISQATSDRMRCIPDVFLSFEK
                     IDIAVEAKLNDGQRSQDAQQLALEWLGYNEEIEGRQMWLMMVSGLEEVTDDCVIGFRR
                     QIEEALRNLGRDGARVQLVGTSWQLIAKALKRVVSAHPIDANILTVINDILEAFQLYN
                     VQSRQPTWLESLRREARPFASIRDESLEQFAGRISNGFS"
     gene            9006..9644
                     /locus_tag="PK224_02310"
     CDS             9006..9644
                     /locus_tag="PK224_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14600.1"
                     /translation="MDSHSDELKLALVDVRKAFRLLFLYQRRILDFSKTFANEMGLRP
                     YSAVYGFNRPTTVNPFDKWVWDMLPMYRVSFLFLSLSTDQNQTKKGEWLLAIHLVSDT
                     GYPDGISWKKGEPNPLDFPVAEDSFSRLGIYAFKSDIDERRNWQESVWRPAINYLPKY
                     PSDWNVVTIEKIGVSICGAQFDLSDLSNKEAMLRTASQFKEVLARDLQLSFP"
     gene            10204..10431
                     /locus_tag="PK224_02315"
     CDS             10204..10431
                     /locus_tag="PK224_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14601.1"
                     /translation="MSRYIYTNWRGKQFVIDEVFADEMKQSLLADGCDVEFVATVTPE
                     SIMKDLPLHEKIERLQQLKHMWEGPVNPRGR"
     gene            10567..11850
                     /locus_tag="PK224_02320"
     CDS             10567..11850
                     /locus_tag="PK224_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRB14602.1"
                     /translation="MFTRRALFKMLAAIGGGSLLPIDGPAGLYRRFRFNQAFPAFPPA
                     PGKTAPSPFGSIPDLSIIEQWENLLDDLDGVELIGRYSRLTPVGDGSRDCRGLCQFCR
                     QGTDSFRVSCRDGSYFCTDCLVYGHALDFYCEMERLTPAEAIPRLTGLLASGALTGKR
                     LRLERMFRVLDEVRRVACEALQHGSAGCAVREWIEREGVSQETGGQFSLGLLSREVRQ
                     GLVLHLRAQGFDQAELEEIGITGWLARHADKSEMSMLIPVGDVDGSCRGFYEQGMYED
                     ADVMWTSYSLPYGFTLLSPHRADRLVLAAEAGRTSSASVVLAERPWDVVLLAEAGIKD
                     AVYVAALDPAEYRQRLNLQLQRVQRAIWPIRQDDSTVEFLRCLTEGFGRSVRQLRFAI
                     LPPGLRLPEVFRREGIESVHARLAGAKPLRELLGF"
     gene            12493..12686
                     /locus_tag="PK224_02325"
                     /pseudo
     CDS             12493..12686
                     /locus_tag="PK224_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            12867..14258
                     /locus_tag="PK224_02330"
     CDS             12867..14258
                     /locus_tag="PK224_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14603.1"
                     /translation="MDKTKMTDFAGSWVGSINGTNIATFTVTLEQTETKVLGDLTLTD
                     AALGIATFKVDGTAGDGVATFRLIPGTAASGVGLSNGVARVALQPDGSLAGIWEMESQ
                     TFGAVTANREIKTEELEPSPGGPLLSGRPELDRLAVGQMPYRWMPIMGEFELAPRSLV
                     FKGRRIPSVAGPAQAEGPQFLAAVGIALCDRKLVNGTLTAAVRFVEISPNSVCELIVA
                     HDLRTKGQITAGLGGSGAMFSIREWLPAQTPQQTGGSWVNYELAGDRANLRAGITYQL
                     VVRLHGSNLVLEVDGVEVAATGLQAAPNQPRQIGVFAVSEGQVRVADFSANIERPTAF
                     IVMQFSPPYNEVYSDVIKQLCEECRIDAVRADEIYGPGIIIKDVIDRIAKSQVVIADI
                     SPTNPNVYFEVGYALALGKPIILLAQRRGPESPLPFDLSAFRVLFYDDSIGGKLKLES
                     GLRKHLKEILGNG"
     gene            complement(14306..14506)
                     /locus_tag="PK224_02335"
     CDS             complement(14306..14506)
                     /locus_tag="PK224_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14604.1"
                     /translation="MADDGIFNKAASIERCPRRITEEYSGNRQNLFEHQTKQDARHAR
                     KAFRGSISSVSLFRGYAVLRSR"
     gene            complement(14894..15010)
                     /locus_tag="PK224_02340"
                     /pseudo
     CDS             complement(14894..15010)
                     /locus_tag="PK224_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(15112..15552)
                     /locus_tag="PK224_02345"
     CDS             complement(15112..15552)
                     /locus_tag="PK224_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14605.1"
                     /translation="MGLISWLASVMILAALTVQAEQGPHRFSVDLPTGWQQASHVRLV
                     LEGMTVAGGQPFKLRAAAIIEGAEPVTLGSAGIVGDRSVGSAPRRLPTLLIDVTRHLA
                     SLADRLGTQPAITIFIEAVDGRNVPIGGLDWSVQSVRLERGGRN"
     gene            complement(15555..16991)
                     /locus_tag="PK224_02350"
     CDS             complement(15555..16991)
                     /locus_tag="PK224_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HRB14606.1"
                     /translation="MSNLDDRMWATMPSRRSFLQRMGLGVGALGAGLFGGLVLPESVL
                     AVCEPPGNPGTPKPWRKDCRMILPRRPASTLSAAEITQLKDAYKAMRALDTSAPNDPR
                     GFLRQANVHCWYCGAGTQVHFTWQFFAWHRAYLYFHERILGKLIGDMNFRLPYWDWDT
                     PSHRKLPGAYSDPNDNSNPLWNGTRSMDPTDEIPEEDVGEDVMEAALTADTFTEFGGT
                     TSGSGIPEGTPHGAVHVDVGGDMGFFDSAGKDPVFYAHHANVDKMWSDWNKASSIHTN
                     PTATAFLNLTWNFYDENKVWRSIKASQVLNHDTQLRYTYGPSKFLEQLPCLLDWFPIK
                     TDWRISQTLKFAGQTRAKMMKVVEQGGRARLHLNELAVPTDKSAVYRLYATPEAAKAD
                     EGPGSKGYLGTVPVVLNDRERRHVSKNARNIAVRLSTAKLEALSGTLGPVRLALVERG
                     VKPEARKVIPVRAKDVLLSVAEVEREER"
     gene            complement(17842..18255)
                     /locus_tag="PK224_02355"
     CDS             complement(17842..18255)
                     /locus_tag="PK224_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRB14607.1"
                     /translation="MADDVVLNKAASIERRLARICEDYGGKREHLYENQTKQDAIVLN
                     LQRACETAIDLAMYVVRRNGLGLPQESRDAFALLEKAQLLSAELAGRLQRMVGFRNVA
                     VHEYTRLNPDIVQAIITKHLDDFRRFSPTIVKACA"
     gene            complement(18248..18535)
                     /locus_tag="PK224_02360"
     CDS             complement(18248..18535)
                     /locus_tag="PK224_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRB14608.1"
                     /translation="MSAAVYFDIFPGVERLFHLAQELAIQVGRNVDLLDLRSTTTVMR
                     AQIISTGRCLKSHDDQARAEFEMYAYSDYARLNEERRELLKNIKERGLIYG"
     gene            complement(18865..19131)
                     /locus_tag="PK224_02365"
     CDS             complement(18865..19131)
                     /locus_tag="PK224_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRB14609.1"
                     /translation="MTKTMSLKQARSRFSSLVDNADRLSERVIVTKNGTPKAVVMGAE
                     EFETWVETLELLSNPQAVKALEQGLKEAKAGKLRSFKDVFDEQQ"
     gene            complement(19192..19530)
                     /locus_tag="PK224_02370"
     CDS             complement(19192..19530)
                     /locus_tag="PK224_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HRB14610.1"
                     /translation="MGEWAKYGLYFLLGGTIVSISTYLGSQGRSFLAAFASTFPAMTG
                     ATFILIYLNGGSEHLVTYAKNLLWFVPPWLVYVGCMIYGVERVGFWLSMAGSMALYMC
                     CVGLVKLLAR"
     gene            complement(19543..20562)
                     /locus_tag="PK224_02375"
     CDS             complement(19543..20562)
                     /locus_tag="PK224_02375"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14611.1"
                     /translation="MNPSFSGYLVNDVFGDPGLFVEVRWSKRALLFDLGHNDALGPTR
                     LLRAGDIFISHTHMDHFVGFDALLRVALGRGKTLRLYGPPGLIANVQGKLHGYTWNLV
                     DGYPLTITVQEFHEQTIRRATFLATDGFQRHDAPEGAPIDRRAGRRFSVLTDPMFTVE
                     AVALNHHIPSLAYSLQEQFHVNVNKERLHEAGLPIGYWLKEVKQYLWQGKPDDFRFQA
                     TLYHEHHKEEREFVLGEVRERFITISRGQKLAYVVDARYDEENEAKIVELAQGADIFY
                     CEAPYLDQDADKARDRYHLTARQAGVMAKKAGVRELVVFHFSPRYTGLGHRIEAEARQ
                     VFRGS"
     gene            complement(20661..21344)
                     /locus_tag="PK224_02380"
     CDS             complement(20661..21344)
                     /locus_tag="PK224_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14612.1"
                     /translation="MIKPDDASSTHATQASERGAALVLLPLTATILFYSSPTTLQQHS
                     LFQFFPQACAYAAFIAWSTINGSVARRIGLARPLVPQGLRWGLLTGLTLGPINVLVIL
                     YLIPILGGDYRFLADTPHAKIPLLIMVPWFILLIATMVELNFRGFLLGRLLALGLPAP
                     IAVPASALLFAFDPFLVATFQHLHWIAVWDGLMWGTLWVMLRNLYATIVAHAVEVIVL
                     YGVMRVILT"
     gene            complement(21352..22902)
                     /locus_tag="PK224_02385"
     CDS             complement(21352..22902)
                     /locus_tag="PK224_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB14613.1"
                     /translation="MTNAILRGCLFIGGAAALLLWTAPDSFGLEVNKPVPTERREAMS
                     LADAVLKALQNNLDISIGRQTRESRLADIVIEQAKFDPTVSLNGQYNRQVSPLNRPIL
                     GFTGANLQEITKFDQNSHSLTADITQNLPTGANYDLNYSPQRSYVSGPNTFLFNPAWT
                     GGLALTVTQPLLKNFGTDINKTFISIAQNNATVEQHVFLDRVLTVIASVEQTFWEMVF
                     ANENLKVAQAALKAAEELLASNRAKAKAGVMSIVDVLQAEAAVASRVEQILVAEKSIR
                     DQEDQLRRLLNPVEEELRQDLRLIPTDPPVTSLEAISLQEAIDIAMERRPEVLQAGKN
                     VESSDLNVKFAKNQLLPTLSVQGTMGLSGLGADYGDATRRNLGGDFYNYGAGLVLSYP
                     IGNRSAYSTYNKRQLESRNAQSSLQSVRQQVIVGVREAVRRVHTDFKRIETTRSARIM
                     AEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAVLDYNKSLSNLARNKATTL
                     ERYSLRLD"
     gene            23008..23376
                     /locus_tag="PK224_02390"
     CDS             23008..23376
                     /locus_tag="PK224_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14614.1"
                     /translation="MAGLTLLLTSPAAAQSVSSVRGLGGGVAPLFGLVGPGNLYIDNQ
                     GTQGYIYNFGNNFESYNFRNPATGQAWSGALMTLGPQLSVGLIQGANQAGSPVVFPPV
                     PREAGPLSPVQSGLLDDLIP"
     gene            24163..25236
                     /locus_tag="PK224_02395"
     CDS             24163..25236
                     /locus_tag="PK224_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB14615.1"
                     /translation="MRIAQVSPLWESVPPKLYGGTERIVSYLTEELVRQGHQVTLFAS
                     GDSVTRAKLEAPCQQALRLNTGIFNREAPLIQMMEQVFASADQFDLIHSHLDFLAFSL
                     SRRCRVPVVTTLHGRLDLPELVPVFRDFAELPLVSISDSQRRPLPWCNWQNTVYHGLP
                     HDLYKFHPEPGKYLAFLGRVSPEKCPDQAIELAKRVGIPMKMAAKVDPADRAYFERVV
                     EPLLDHPLIEFVGEITDAEKSDFIGNAIGLICPYDWPEPFGLVLIESLACGTPVLAYR
                     RGSIPEIIGHGVTGFISENLDEMVSQVDKLGTIDRNRCRQVFDERFTAQRMTTDYVKI
                     YQQLITDAAALPGKSGQQHASNL"
     gene            25270..26550
                     /locus_tag="PK224_02400"
     CDS             25270..26550
                     /locus_tag="PK224_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB14616.1"
                     /translation="MAAESQSTPESNVDGWRLLGTRDFGCLWAGQVVSQIGDGLNKVA
                     LLWFVYEMTGSALKMTAIGLLQTIPPLLFGPLIGVYLDYLPKKTVMIVVDFLRALMVL
                     LIPLFYTFDMLTLERLYVLVFLISIVSTVFGPALASAVPLIVQRSQLTTANAFLQSTT
                     NIGVLLGPAMSGLGIALIGAQNVLYVDAATFLVSALFLLPIRVRDSRTVKGLDVLATP
                     VMQDMMVGFRFVFLQHRVVFALMITAVLYNLAISAFVFLLPVVAKELLQVGPMELGWL
                     WSALGIGMLAASIWLARTPQGTFQDRIGKIGRSLAIGGIAVCALGLIQTPVLFSTFLL
                     IVIIGGSTSLFYPVVWAMLQEVTPEHLLGRVFTTFSVGGMASAMVGMAGFGWAADTLG
                     PAVSLIGIGLLLLLTAMVTVQVSRRGHVVSPAVA"
     gene            26785..27789
                     /locus_tag="PK224_02405"
     CDS             26785..27789
                     /locus_tag="PK224_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRB14617.1"
                     /translation="MPMRVIRTVYVTGVLALLAASGVGNVNETALAESTPEGKSETPP
                     VQRPKDPDTKPKEPEPKTKEADSKPKESDAKPPEKPKEAEAKPKEPESKVKEPETKAK
                     DQEPKAKEPAAKAKESEHKVKEAEPKSKEPESKAKEPDAAAEHPCPTVPHAAEVKPAA
                     ETQAPPGEPGAGERPKAAEPEGKKPIRSSMVTAKLALMADPHLFPYDIEVDAKDKDLV
                     LLGKVSQESDKRVATDIVRCLDGVHAVENRLKVEQDAAHGLFAERDKIITQLVKERFE
                     KSKTLQSVKFDVKTEDGIVTLNGATRFQIIVLEAAQAARQVPGVRAVNTDAVRLVAGE
                     "
     gene            27859..29184
                     /locus_tag="PK224_02410"
     CDS             27859..29184
                     /locus_tag="PK224_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB14618.1"
                     /translation="MNGGGTGRPNVKWSPHLLTRDFTLVWWGQMVSQVGDGVSKLALL
                     WFVYSITGSPLKTTMIGLLQTLPPILFGPFIGVIVDRVPKKLLLISSDLIRALVLGVL
                     PCLLPVDSFSIERLYLMVFVHAVASAVFGPALTAAIPSLVSRHEFTAANALLQTTTSI
                     GIIVGPALSGVGIATMSSQEVLCVNAVSYVISAACFAFIRFPRAESQPVGGGSLAGTF
                     SDVLDGFHYVLHRQRVILMLIGAASMYTFATSAFSTLFPVFGKKLLDLGPIEVGYLWS
                     AFGIGLLLVSLGLVSLSAWSLPKRIQLMALSSFISGLALLGLIVTSNRLIAAALMVII
                     GMGAGTLTPVAWGVLQEIAPASLLGRVLAIYNLGAMTSAIAGMTIFGWVTQDFGERPS
                     VFGIGVGLFLSALVSMRVARWVQTNWAGATVPSEEESQSVVMVTQPTSH"
     gene            29336..31501
                     /locus_tag="PK224_02415"
     CDS             29336..31501
                     /locus_tag="PK224_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRB14619.1"
                     /translation="MEEIISVNDQFYILASSSMADDRTRVLKHGETFGVYDRYGDIQP
                     VGRGTQGVFHQGTRFLSRQELFLNNDRPMLLSSTVKEDNALLAVDLTNPDLYRDGRIA
                     IPRGSVHVFRSRFLWNGVSYERFRLSNYSLAPVKMTLSIRFEADFADIFEVRGKKRER
                     KGRMLPNVLRKDLLVLRYEGLDEVTREARIQFSPEPLEITASQATFDIELDPKGETTV
                     AVMVACDVGDSHRPLLAYDAAMAEAGLAFGAEQTEDCTIQTSNAQFNEWWNRSVLDVR
                     MMVTDTNEGPYPYAGVPWFSTPFGRDGVITALECLWIRPELARGVLAYLASTQAKEVN
                     AGQDAEPGKILHETRKGEMAALNEIPFGLYYGSVDSTPLFVMLAGAYYERTADLAFIQ
                     SIWANLEAALTWMDTFGDPDRDGFVEYVRKSPTGLDNQGWKDSHDSISHEDGSLAEGP
                     IALCEVQGYVYDAKVQASKLADALGYIDRASQLRRQARSLKERFEEAFWCEESSTYAL
                     ALDGQKRPCRVKTSNAGHCLYTGIASDEHARRVAETLMSDELFSGWGVRTLADSERRY
                     NPMSYHNGSIWPHDNAMIAVGLARYGLKSGVEKIMTGMFEVSLVLDFHRLPELFCGFV
                     RRPGQGLTRYPVACNPQAWAAGSAFMALQACLGLSIIASEQKVVFNYPILPEFIDKMQ
                     IKNLKVGTASVDLLLRRHDLDVGITVIRRAGKVEVVSVK"
     gene            complement(31591..33075)
                     /locus_tag="PK224_02420"
     CDS             complement(31591..33075)
                     /locus_tag="PK224_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRB14620.1"
                     /translation="MALATGQSIAAETGAPSTALTQALSQISSERMLADIRALSGPAF
                     NGRQTGTPDDLASAEFVRQRFLELQQHRSPGSESPAPPDRLPAHTQIQSAPVRITQID
                     DDSLLQIGGIAESQPSVVGTDYLPILDSPSAELETSVVFAGYGISDQAGGLDDYAGID
                     VRNKVVLFLRGKPERYAKQVSHADKVHMAHAHGAIGYLTATGPILNAYETRRGVTGRP
                     SAFYGLTDPHRTIPGAWISTALASAILNAHQPGEDNRLRRLQQQLNDTMAPQSTTTDV
                     SIEVRWRSTQQNGTLQNVVALLPGQDAAHQHDAIVIGAHRDHFGKQGGLLFAGADDNA
                     SGTAVILEVARVLTSMPVGPKRSIIVVSFSGEEQGLLGSKLYVTQPMVPLTATAAMIN
                     VDHAAVGNGRLTIGVTGLEKPAAQQAGERAGLADRIDLFGFFPGGDHVPFKEAGVPTV
                     TVVSGGIHPHFHQPTDTADTVNADILSAAARYILTIAWQLADAP"
     gene            complement(33183..33620)
                     /locus_tag="PK224_02425"
     CDS             complement(33183..33620)
                     /locus_tag="PK224_02425"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRB14621.1"
                     /translation="MDLSQQPPRRWSDTLAGIMWLPRLIDKVRAFQAGTLGTYAYPSA
                     LDQSFMRRFQLTPAYIEPLVREAASDEAIGTAIRARIQLSDEEVQHRCAIFRDKYRLA
                     FAVLDRDDGYVRGLGYPIPRFLQPPLWRWYQRWSAQKASATSI"
     gene            33818..34063
                     /locus_tag="PK224_02430"
     CDS             33818..34063
                     /locus_tag="PK224_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14622.1"
                     /translation="MAEQGKGLYDHQYGRFRENQGTHQGYEFQHGPAGSVPPPVVTFM
                     DKIKWWTLRGWRRKKILAERDRFQQDIIRIKQTQPKS"
     gene            34090..34920
                     /locus_tag="PK224_02435"
     CDS             34090..34920
                     /locus_tag="PK224_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14623.1"
                     /translation="MQYGHELVRQIEFLRDSQSLAMAHLRNIRRYAGVIVLTLMSVSG
                     VESVLAVPKETSTEKAEHLRQQANPQLFNNWTFDKDQAGGVPKGFLAVASGDQPGGDW
                     KVEAQSAAPSSPNVVLGASSCPACVELLVAQEFQYEYPELVVRIRQGADATSGQIGVV
                     FGLKDPKNYYATVVDLSQKSIEVVRVIEGKESILGRAAIKSKPVEWHTLRVQRNTIIS
                     KDFVETFFDGSLALSVEDQTLGVGQVGILVRGQATARFDNFNASPLYSSRPLSAPAAY
                     "
     gene            35197..35496
                     /locus_tag="PK224_02440"
     CDS             35197..35496
                     /locus_tag="PK224_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRB14624.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAVHGAVTSARIITDKFTGQSR
                     GFGFVEMSGDSEAQAAINALNGTQFGGRTLTVNEARPQEPRSGGGGGRGMGGGRH"
     gene            35645..36997
                     /locus_tag="PK224_02445"
     CDS             35645..36997
                     /locus_tag="PK224_02445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB14625.1"
                     /translation="MVSFAELSLTSFLADRLQQAGFTAPTPIQSAAIPLALEGRDLLA
                     QAKTGSGKTLAFLIPLIERAVKEGWKPAGHASASHAGSARSPRALVLAPTRELALQIE
                     MELRKYAPPSVTSLAVYGGVPIERHYRALRQPPLIVIGTPGRLLDVAGTRHLDLRGVE
                     YVVMDEADQMLDRGFLRDIQRILQLLPTQRQTMLFSATFSPEILTLAESMLKNPARTA
                     VDPGVNTPTTITHAYYVVPSESSRVQLIHTLLQSPESGDQSMVFCDQKYKVKRLAARL
                     GGEPASVGAITGNHSQAQRERTLTAFRSGRLRTLVATDVAARGLDVPSVSQVIHYELP
                     GNPTSYVHRTGRTGRAERSGATLLILSPQEEHEYLAMVRRLRIQTKRLTLPALAVLPP
                     PAHEPESNGQSRHDGRGRDARPRRAGERQNSMPQDARGGQGRRGWRADRPAAPRRGNS
                     "
     gene            37054..37587
                     /locus_tag="PK224_02450"
     CDS             37054..37587
                     /locus_tag="PK224_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRB14626.1"
                     /translation="MKAGRSLLLGMLLAVGGQLMGAGAYAQGDLAIADGVKVSLEYIL
                     TLPDKSVADSNVGQEPIIFVQGAHEIVPGLERALDGMKAGQKRRIEVAAQDAYGPYNN
                     KLRQSVDKEKLPKDVKVGDILQASDNRLVKVLEVNDKKVLIDLNHPLAGKTLTFDVNV
                     LKVEKGDAADAPASKTP"
     gene            37847..39112
                     /gene="pfp"
                     /locus_tag="PK224_02455"
     CDS             37847..39112
                     /gene="pfp"
                     /locus_tag="PK224_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRB14627.1"
                     /translation="MGTIKQRPVVAILVGGGPAPGINSVIGAATIRSILGGCDVIGIQ
                     DGFKWIMDGQTSKVKRLSIEDISRIHFSGGSCLGTARANPTQKVEHLDACLTSLNALG
                     VTRLITIGGDDTAFSALKLEQRAAGRLQVVHVPKTIDNDLDLPHGIPTFGFQTARHIG
                     VELVKSLMVDAETTSRWYFVVTMGRKAGHLALGIGKAAGATLTVIPEEFRQKPLRLKT
                     LVDVLAGAFIKRLNAGRSDGVAVLAEGLVEILDQQDLDGLQDVERDQHGHVRLAEINF
                     GFVLKRAVEKELRSFGIKTTIVEKNIGYELRCADPISFDMEYTRDLGYCAAQFLLEGG
                     NAAMVSIQNGRFIPIPFGDILDPATGRTRVRMVDVESESYVIARRYMIRLAPDDFNQP
                     QALVRLATTAGLAADDFRKRFEYLMGKDL"
     gene            39164..40117
                     /locus_tag="PK224_02460"
     CDS             39164..40117
                     /locus_tag="PK224_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14628.1"
                     /translation="MTSEATPDTEIALACGQQRALVSPWGAALRRYYLLEDNGRETDI
                     VWGYAGGAHKKGGQGDVLIPFPGRVAEGRYSFDGQQLQLERNDKEGPNAIHGFVRTLP
                     WTTPQADSDSVAFEVQLEAANYASRGYPFSLAVRVAYRLDRQGLTCSFAVHNIGREAA
                     PVGVGFHPYFTVGTALVDEAEARIPASGYLEFNDRLAPTGKVLDAQDTEWDFRECRRI
                     GNLRFNHCYVGLERDAEGMATATLQHQGSGRRIEVVMDRSFSAIVVYTGDAIVAAPRR
                     ALAIEPMTCATDAFNHPEWGLKRLQPDETFTGRYRVTHRLL"
     gene            complement(40200..41954)
                     /locus_tag="PK224_02465"
     CDS             complement(40200..41954)
                     /locus_tag="PK224_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB14629.1"
                     /translation="MLIHTCSPVRAPHPFIRLTYHIIGFLLLLLPVTLTDAPRAMAND
                     QGLHSESQVAQRAKAAWEQGTTDLALGILDQGIHENPQAFALHQLRGDMLATSRHTEA
                     ALQSYETVLASIPTALDVRWAKWSVLVRSGQVEEAVIELQRIAAIDPQNPLIHLRLAR
                     ELRKLDRLEESFKSYQQAVELGPDMLNWRLAMARARFDILDYEGADREVQSVLQQVSP
                     GSPLELSAKNLLTVFYGSTERGRRFAPMMSPDANPKQLKDWSMIRHDAYALFEAGRYQ
                     EAEPMYRQLLLLNPMDPLAKQHLGLILMNLGRCKEAISIIPKMEGLDPLDEDYAATVY
                     RLGQCLVDLGKWEDAYIQFKILYDTTVAFEQSTREVELPIGTRVLDKNKLLRWLDKIR
                     PHVPELAKAIEMEDAAPVTPAAPVVPSEAELAAKAMENLKPQNTLDTAASLVGRDADF
                     AWFRFVIPAKKVMRDDSPTGAHDFIPLDPGISFPATQQDIYLVFGLVTASYDEVPLAA
                     RCFKERTELTGAQPAVAQDQLIMSTNDQSGYFVLSRPSTGWTTGLYRCGLFAGEQTSA
                     YTQVDEVRFRIVESTKPS"
     gene            complement(42263..43825)
                     /locus_tag="PK224_02470"
     CDS             complement(42263..43825)
                     /locus_tag="PK224_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB14630.1"
                     /translation="MSLSTSVHDLRTLIRSCHPLIVVETVEEERVLALLQSVAAQERM
                     PLFEWSITRGLTRQDEGQSISKMTATPLAVLQHLNGLTVEALFWLKDLAPHLQDAAVA
                     RQLREVSHHFGRSRTTCVLTGHPIVLPSDIEQIAVRLDLQLPNRNELQSMLQSVLQSL
                     GTRTSPRRPGSTTVVQSMLHSLAGSKPTQATPSTQESDAILRALRGLTLHQARQVITQ
                     CLVEDGRLSAADVQTILKRKVQAIKDGGLLEYYPLEDNRFELGGFVNLKSWLERAKVG
                     FTAEAKALNLTPPRGIMLVGVPGCGKSLAAKAIAREWQLPLLKLDAGRLFDKFVGESE
                     KNFRKAIEMAESLSPIVLWIDEIEKAMVAGGGSGDADAGLSRRLFGAFLTWLQEKKQE
                     VFVVATANNLASLPPELLRKGRFDEIFFVDLPDDHERAAIWKIHLALRKQDSAGFDLE
                     KIVSASDGFSGSEIEQAVVAALYRALHHKTPLTTDLLIQELSGTVPLSVTRHEDIDQL
                     RTMAHGRFVNVR"
     gene            44051..44914
                     /locus_tag="PK224_02475"
     CDS             44051..44914
                     /locus_tag="PK224_02475"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRB14631.1"
                     /translation="MTSNPENLCDDEDKLAPRIATFWRRIGAAVVDASLLGAIGACLG
                     FLWFDQLAALGRTGRFIGGAIALVYFGTLNSRIGQGQTLGKRLLKIRVTDAKAALVSL
                     PRSAFRATILLLPVALNGMPVPAGEHEQLWGIVLSILIFGVSVAGVYLYSCNRRTRQS
                     IHDLAGGTFVRNAESTSEIYEEIWKPHFAIAGGLCLAVLGVVLMPYTSEQPEFVQQLA
                     EVRRVVESAVPDADVSVQGGHNQGQRINDWADRYFKDRYRRVRVGKTRGFRDGGGPNC
                     HGDVQVFVRRS"
     gene            complement(45176..45592)
                     /locus_tag="PK224_02480"
     CDS             complement(45176..45592)
                     /locus_tag="PK224_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRB14632.1"
                     /translation="MIRAVLDTNVVVSALLFSGPPSRLISAWQSGRLPLVVSAPILDE
                     YIRVLAYPKFKLTNTEIRGLLEEELIPFMETVTAVPTNVPDLRDPDDAKFITCAVAAG
                     VRWLVSGDDDLLSLHHVESVDILSVTAFLQQLKRRS"
     gene            complement(45589..45831)
                     /locus_tag="PK224_02485"
     CDS             complement(45589..45831)
                     /locus_tag="PK224_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14633.1"
                     /translation="MLAKKTSKNQVTLPKKALQDIPETDYFDVTAKGGVLILRPVTMA
                     EPGSRLTAVRQKIKELGIEMKDVDRAIAWARGRRRR"
     gene            complement(45901..46761)
                     /locus_tag="PK224_02490"
     CDS             complement(45901..46761)
                     /locus_tag="PK224_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRB14634.1"
                     /translation="MRDERTTNYYAILELSPGASDAEIKRAWHEHMQVWHPDRFVHSP
                     TLHRKAEARTQLINQAYQTLSDPAARARYDAGRQHPSSPTPPPRPSPTPRPQAAPRPR
                     QELRGPQTMLNVTRFSHPKIMVPAIHMLVDSREHLPYEFKGLVRIAGTITQTLPAGDY
                     AIAEAPAIFCVERRRVEEFNTIFSNPSDNRPRFLRELEPLLACPHRFLVIEGAIQYNS
                     GGGRLGQYHRNGMVDFLDALTARFGLQIIYSDSREEAEERVANLAALHYAYHLAEQQG
                     LGRCLTENDA"
     gene            46960..47979
                     /gene="galE"
                     /locus_tag="PK224_02495"
     CDS             46960..47979
                     /gene="galE"
                     /locus_tag="PK224_02495"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRB14635.1"
                     /translation="MILVTGGAGYIGSHTCVELLNAGCAVTVFDNFSNSHPEALARVE
                     RITGKSLRVIRGDCRDRAALVAALRESRATAVIHFAGLKAVGESVQQPLAYYDNNVVG
                     SLRLLEAMRECDVQQLVFSSSATVYGDPQRLPLTEDHPLSATNPYGRTKLMVEEILRD
                     LQRSDASWRICILRYFNPVGAHASGLIGEDPQGTPNNLLPFVAQVAVGRRECLNVWGN
                     DYSTPDGTGVRDYIHVVDLALGHLKALEALDRLERPGECLTVNLGTGNGYSVLEIVRA
                     FEAASGKPVPYKIAPRRPGDVASCYADPKQALKSLGWEAARGLNEMCADAWRWQSSNP
                     KGYAG"
     gene            48021..49292
                     /locus_tag="PK224_02500"
     CDS             48021..49292
                     /locus_tag="PK224_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14636.1"
                     /translation="MKTIGLQSEPVLDCRQVSVLHITTLLLRAGLIILASRGASWAIG
                     AGAVACALLFVPAVHANHQGHVSHPDGEAHITVGAGYSIPGASFQIFMGPGNDAAEET
                     GLTQADRSAAVETVREAFAVLVQHRNDYPRFDESLKKEALARVVIETTVVNDEGKAFP
                     FLVARTTEPGRVILLISASSLKEKGYLHHPDTLAPVLAREFQWVVSKADTAPKPKMVA
                     GERALKSAPIRTDQDIAALSGEERVRLLQQLLGSYLRTVDDQRSLDGQSYYEVRSTAL
                     VAPTHPDSTTKLYEIRVREALQRIVREQVFWERTPKAVRSLLNGKVWTVAFVNIDQRD
                     WATRTRVLPEEKAVIVGARDQRVQPAAILVNTYRTAAPDDPFYPDTQGLPMGALSAEQ
                     LARVIALEIQHNIQEKSMAGHVAQDALTAPK"
     gene            49924..50997
                     /locus_tag="PK224_02505"
     CDS             49924..50997
                     /locus_tag="PK224_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRB14637.1"
                     /translation="MSRFVSALCGAVLALLAVLPGNAAAAPSSGSSWEPMRIMHFVLT
                     GTVPLERAREYVNQAQAAGFTAVQVVLTDGVRLDHAPWKPVKGAWTKAEFLSWAAYAR
                     AHGLDVIPEVKLLTHQEQFFQKHYPGLMFNAVSYDPRKEGVYQAVFRLLDELIDALHP
                     RAIHIGHDGAFGWTVGQVAKWLKFGEVMVPADLFARDVERIHGYLTQKGVETWMWADM
                     LLDPAEFPGASPKHLHGVAAGYGKALRNRLPRDIVMCDWHYGDEQGSFPSMAVMQSEG
                     FRVIGATWKREETMRNFSRYALSRHAYGLMATTWFHVQRNDRDLVDWIIRSSGMLFRN
                     PDAAVPPRPASAAPPEGHGWTAP"
     gene            complement(51088..51780)
                     /locus_tag="PK224_02510"
     CDS             complement(51088..51780)
                     /locus_tag="PK224_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRB14638.1"
                     /translation="MPRSLPLSLSLSLNLLLIGITLVGWVAPNVPTTFNPHVAVPTIA
                     ASARIHPLAAVIGSVTIGELIFIAPGASVRGDEGQNIYVGDHSNVQDGVVIHGLETFE
                     GDHELPENEVQVSGKTYSVYIGKRVSLAHQSQVHGPARIGNDTFVGMQALVFRTELGD
                     HVVVEPGAKLIGVKVASGRYVPALTLVTTQEQADTLPLITSAYPYRNLNEGVVTVNVQ
                     LAKAGQPKQIGR"
     gene            52190..53281
                     /gene="ychF"
                     /locus_tag="PK224_02515"
     CDS             52190..53281
                     /gene="ychF"
                     /locus_tag="PK224_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRB14639.1"
                     /translation="MSVKCGIVGLPNVGKSTLFNALTKSGIAAENYPFCTIEPNIGIV
                     EVPDARMQALADIVKPQRMQYATTEFVDIAGLVAGASKGEGLGNQFLATIRETDGIVN
                     VVRCFEDDNVVHVAGKVDPISDIATIVTELALADLTTVEKAQERNVKLVRSGDKDAAK
                     LGELLVQVAACLNEGRPARTLKLDPAQRALLKPLCLLTMKPVMYVANVAEKGFANNPL
                     LTRVEEYAAHEGAPVVAICAALESEIAVLSDEEKREFLADIGMTEPGLNRLIRAAYQL
                     LGLQTYFTAGVKEVRAWTIHIGDTAPQAAGVIHGDFEKGFIRAEVIGYNDFIACKGEA
                     GAKEKGKMRLEGKEYVVQDGDVMHFRFNV"
     gene            53339..53794
                     /locus_tag="PK224_02520"
     CDS             53339..53794
                     /locus_tag="PK224_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRB14640.1"
                     /translation="MNEQDTQRAVAILNRIMEHELAGVVRYTHYALMVYGYNRIPIVS
                     WLKGNADESLAHAHKAGELVTLLGGHPSLKIGTLLETEKHDVGDILRESLEHEKAAVA
                     SYYELLKLTEGKSVLLEEYAREMIVGEELHLDEVNKMLRKSGDVQAFKS"
     gene            53923..54387
                     /gene="trmL"
                     /locus_tag="PK224_02525"
     CDS             53923..54387
                     /gene="trmL"
                     /locus_tag="PK224_02525"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HRB14641.1"
                     /translation="MFNVILYQPEIPPNTGNIIRLCANTGASLHLVKPLGFTLEDKQL
                     LRAGLDYHEFASITVHEGWAECLARMEGRRIFAVSTKGRQRYDLVRYIEGDAFVFGPE
                     SRGLPAEILGSVPEQQCLRVPMVPGSRSLNLSNSVAVVLYEAWRQVEFWKSV"
     gene            complement(54473..55660)
                     /locus_tag="PK224_02530"
     CDS             complement(54473..55660)
                     /locus_tag="PK224_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB14642.1"
                     /translation="MNAQRKAQLEPSGPKKILVCDGGGILGLMTVEILAKLEADLRAR
                     LGKPDLVLADWFDFVCGTSTGAIIAACISAGMSMNKIRTFYVESGEQMFDKASVFKRL
                     RYSYNDEPLAAKLRVELNRALGYPDGAPPATLGDAGLRTLLMMVLRNHSTDSPWPIWN
                     NPKAKYNQLDRKDCNLHLPLWQLVRASTAAPTFFPPEVVTFAPGTPDEYQFVFVDGGV
                     TTYNNPSFLAFQMATAAPYKLHWSTGTDKLLIVSVGTGCSAKARLDLQADDLWLLDHA
                     KHVPGALMNAACAGWDMACRLLGECRFGAQIDREIGDMVLAADAATSNWTGAKQFTYV
                     RYDPDVSQTGLETLGLGNLSAEHVQVMDSVKYIPDIQRVGEAYAQKHVRLSHLHPFSQ
                     TSA"
     gene            complement(55745..59278)
                     /locus_tag="PK224_02535"
     CDS             complement(55745..59278)
                     /locus_tag="PK224_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14643.1"
                     /translation="MRDPPLQQVMRRLREELDKEKAEYPEPRSESSLWEYLHRKDLKI
                     WSCDNFLLTPVLVFDQFEELFSQKGSDMELIKDVIYNLADLIENRIPPEVIKEGAGTV
                     RASLDLLSQRYRIVLSFREDFLPEVQAWEQKAPSLLKNYLRLSPMPRERAIEAVTQAG
                     EEVLDANVAPLIVDLVGKGDHAADATNMSEIVIEPVLLSLCCSRLNARRADGAKIDQT
                     LVEHTGQDILDGFYREALEDPAVKGLPDAALFIEDYLIQGDHFRGAFPKDEALGNKLL
                     TEKQLHTLTDQHRLLRLVHHPDTTRVELIHDRLVPVVRKARDERKIKQHQAEQERLAK
                     LAQEERDRERARSEELQAERDMARRSLKVANLMRNLATAAVIITVILAGWGIKQQMKT
                     AAEAQKRAEIEANTTVALVTSRLAEGRLALASGMEPLEQMMYRGLAAYRLTQGDRKLS
                     QARAASLTALHSVMESAPHLRKAVSIPGLVPTPALAYSPDGKTLAVGGEDGTIRLLDA
                     ETYREMGKLDCGKPTAESLSVWTLAYNSGGTRLAAGYLTSNDQTIGNGFVCVFDVLQR
                     KTLHRFKKDEAGDIYSVAYVGGPEAEFVVSGGSDKMVRQWDLNTGTMSELPHSDKVIS
                     VAVSPNNRLVVSGDEKGIIRLWNRDKPTEKPVELTGHGATVQQVTFSPTDPNVLISAG
                     DDGLIIVWNLKERCRTQQSKQQPARIVNFSLSPDGLIAAASADGYVRVFRLLENVTPC
                     SNPATKGKSATAPSTPEFSLIQDGELVGHGGIAMGIAFNPGGTRLASTGQDGSIRIWG
                     HKTPGFSLAQLQLSSDAQQAVPLPGNVSAIAIGPNGESVVAGDDNGTIYLWRSFHQPD
                     FEPITLPSDQHWHAHDQAIRSLAFLHIGNRAVIVSGGDDGLLKRWDATSGQAIRTEIE
                     DQAEPIRSIVVSPDKQLVAAGSRDGTIRLWDIATENNIRRYERPIDAQEDYELHTLSF
                     TADGKYLAVGDSSAGVRLLDVAKPESERILQGHIDSIQSLSRGSARWLLSAGADGRIL
                     EWHPTILDQPTVEGLRKHDEFKSRLGFRDSTPLTSIDTSGDGKLILIGGRKGRLELWD
                     GDEHVQIATHFPGHQSSDIQAVALAPDGKFFVTADASTILLWPGPDQWADIICSKLDR
                     NMSDDQWHEWVSKAIPPEIQCRGLPGPPAPE"
     gene            complement(59545..60900)
                     /locus_tag="PK224_02540"
     CDS             complement(59545..60900)
                     /locus_tag="PK224_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRB14644.1"
                     /translation="MYKIRDNGVIPIIGSRLLVGADGQTSLQAQVAARLLHDYGKKPG
                     EIALPLFREINEVVSHLKGEAKLQDLYADLSRVIREVITAEDFVIPAPITQLAAIADF
                     RLFVTLTPDDLLARSLRRRRCAVNEIIHSPNMSDRGGNDLPKDWDKGPSEVNVLYLFG
                     KAHADASFAIHDEDVLEYAHNVIVDGHRILNKFLGVLQERSLLMIGCNFPEWLSRFFL
                     RATNRQRLASTYRRSWLIEQLKPEESFTCFLNSYGADTEVLSDLSPVEFVAELHRRWM
                     DEYRAAAQEPERPAEESVPCRAMFFISYSRKTDLPRAEMLYQSLRKLGVTESELWFDR
                     QSIEPGQNFPRRILDGIRSCEYFLPLLSKSCDSRKEAFVFKEWDRADARLPMNREFIF
                     PIIVDDDFTPTIYTFETVKKWRDDNQFDFAHAPEGVPDARLEAKLKKLVRKFRDEGRP
                     S"
     gene            complement(60951..62933)
                     /locus_tag="PK224_02545"
     CDS             complement(60951..62933)
                     /locus_tag="PK224_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14645.1"
                     /translation="MKPHAFVAMPYGVKPDSHGHEIDFNRVYAELIKPAMEAAGLEPF
                     RADQELRAGDIRTDMFQELLIADLVVADLTLDNPNVWYELGVRHALRARGVVLTCGGR
                     VPTAFDLYTDRKLRYSIKDRGPDPATVEADKKNLADMVTATMESWHGRKVSPVYQLMP
                     NLQEPDWKSLRIGDVREFWQQHETWASRIALARKAGHIGDVLVLADEAPIAAFRAEAW
                     IKAGEALRKAERFGFALEQLERGLAVEPRNLKGLREQGICLQRLALAGRPGYSLDRAR
                     AHYRKILELYPQDPETWALLGRVDKDAWIAAWRRSGNSPEQMQDEAAYEDALLRAAID
                     SYATAYRRNPGHYYSGINALTLMHLHRHLIIKDPRYDGEMDAMAGAVRFAAESEPDEQ
                     QRFWSNMTLGELEVLVGTPEAAQAAYKEALAKNDKDWFALNSSRAQLQLLKDLGFRPK
                     NVEAGIATFNCALQKLQKPEDWQPRQVILFSGHMIDAPDRPSKRFPAEKESVAAQKIA
                     EALDQLGTGPEDLALTQGASGGDLLFLEACRQRNVRLQLLLPLPEPEFIQRSILPSAD
                     GDKWRERFYRLKSGLKDEPRIMPDELGPLPDGVNPFERCNRWLLYTALSQGVTRVRFL
                     CLWDGAGGDGPGGTAHMYEEVKRRTGQVTWLDSRKF"
     gene            63741..64427
                     /locus_tag="PK224_02550"
     CDS             63741..64427
                     /locus_tag="PK224_02550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB14646.1"
                     /translation="MKTFQLITVAMATVLCVSLSGCGSGLREKKIEFVYLALGASDAI
                     GVGATPLTEGYVFLVKQELDRRMPGVFLINLGIPGARIDLIKEQVRVAKQLGTKAHLV
                     TLWTGANDLVHGDDPKTFRQDLRFILQTLREEVSPHIVIGNLPDLTRLPRFQRAPDPR
                     VTTARVNAYNEAITQEARDVKAFVVDLFAQPVREELIFDVDGFHPNDAGHREIARLFL
                     QAILAKSGVK"
     gene            64435..64749
                     /locus_tag="PK224_02555"
     CDS             64435..64749
                     /locus_tag="PK224_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14647.1"
                     /translation="MAQTGYKTLAEAIEGLRERGFAANFELLNKTFLAVNSGRTFTAD
                     QLTIVEHHRFEGASDPDDMAVVYAIEARDGTRGTIADAFGPQANPDLGAFLKNVNMHE
                     DL"
     gene            complement(64800..66683)
                     /locus_tag="PK224_02560"
     CDS             complement(64800..66683)
                     /locus_tag="PK224_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRB14648.1"
                     /translation="MPKHLNNQSSSVSLTVAAMGVVYGDIGTSPLYALRECFHESHGL
                     PVTPDNVLGILSMIVWSLVLVVTVKYLLFVMKADNEGEGGMLALLALSQQSRPVDLRK
                     GLNVVVTLGLIGVAFLYSDGIITPAISVLSAVEGLTLTTDVFTPYILPLTVALVALLF
                     MIQRRGSGTIGSIFGPIMLLWFVTMALLGLHSLIQTPEVLRAANPLYAIQFLIRHAGV
                     GILVLGSVFLVLTGAEALYADMGHFGRRPIQLGWFVVALPALLLQYFGQGALLMRTPA
                     ALANPFYFLAPDWMLYPLIALATMATVIASQAMLSGAFSLTLQAVQLGYLPPLRITHT
                     SAEQHGQIYFGLLNWFMFIGTVGLILTFGSSSNLAAAYGIAVSGSMIITTLLMYRVVQ
                     AHWQWSPLPAAMAVGGFLLIDLAFFLANAQKIPHGGWFPLLLGAAVFTVMSTWARGRA
                     IVADHLREQFPPLLQFARDVLSQVQCRTAGRAVFLSQHPDITPPALLQNVRHNKSLHE
                     QVYILTVRTEPVPYVPSGGPLEITTIQENLFQVVARCGFMETPDIPRVLTYLAARGHT
                     LPVEETTFFLSRLTFLATPKPGMAIWREKIFVFLSRNTQRASSYFHLPAEQVVEIGLV
                     LEI"
     gene            67007..69406
                     /locus_tag="PK224_02565"
     CDS             67007..69406
                     /locus_tag="PK224_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRB14649.1"
                     /translation="MTSPTTPTISSAAQQRIVDLIAKELGVTAPQIAAAVTLLDGGAT
                     VPFIARYRKEATNNLDDTHLRTLEERLLYLRELEERRQTILASIDEQGKLTDELRRAV
                     EQAATKQAVEDIYLPYKPKRRTKAQIAREAGLEPLANALFADPTLDPDQEAAKYVKVV
                     AAAEGVEAVNVPDAKAALEGARDILVERFAETAELLATLRTKLWNEGIVTSTVMPGKE
                     TAEEEKFRDYYAYSETIRTIPSHRALAMFRGRTLGVLKLDLGLGESLEAIVPHPCAAM
                     IAAHFGIENRGRRADKWLTDVCYWAWRVKVHLHLSTELLLQVREAAEAEAIKIFGRNL
                     HELLLAAPAGPKAVLGLDPGLRTGCKVAVVDATGKLLDTATIYPHQPRNDWQGALATI
                     TQLVLRHGVELISIGNGTASRETDKFAAEVVKLVAEQKPEQKLAKIVVSEAGASVYSA
                     SAFAAAEFPSLDVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDVNQRALARSL
                     DATVEDCVNAVGVDVNTASAPLLARVSGLNRVLAQNLVEYRDTHGRFQNRHMILKVPR
                     LGEKTFEQAAGFLRINDGDNPLDRSAVHPEAYPVVERMLARLNKGIAEVMGKPAALKE
                     LSPAEFTDETFGLPTVRDILTELEKPGRDPRPEFKTATFRDGVESLADLQAGMVLEGV
                     VTNVAAFGAFVDIGVHQDGLVHVSALANKFVKDPHEVVKPGQIVKVKVLAVDVPRQRI
                     SLTMRMEDAATPPLQPDPRVGGPRDTRDRRPADQQRGGSREPQPIGAFALALARAKEK
                     K"
     gene            complement(69445..70047)
                     /locus_tag="PK224_02570"
     CDS             complement(69445..70047)
                     /locus_tag="PK224_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRB14650.1"
                     /translation="MTRPGSSGEQRAQERFGTSARAAAFYAHQTLPHLNPQMQQFIAR
                     MDMVFIATADGKGACDCSFRAGEPGFVQVLDEKTLVYPEYRGNGVLASLGNILENPHI
                     GLIFPDFYQSTVGLHVNGTARVLNPSETESLSQLPARIADAAHIKGGRHPKAWVLIGV
                     EEAYIHCSKHVPLLKRLDKQIAWGSDDEQAKGGNFFKVTP"
     gene            complement(70160..71518)
                     /locus_tag="PK224_02575"
     CDS             complement(70160..71518)
                     /locus_tag="PK224_02575"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB14651.1"
                     /translation="MTMEHMRALIVDDEEFVRLVVEQALREEGCDTQTAGGGQEGLDR
                     LRTASFDCVITDLRMPGLDGRAILRWVKEHQPDVDVLVLTGHGDVKDAVAAMKDGAWD
                     FLIKDTPFDGAAVKAALARLRTVRELRRENLAARHGGYRQDAIVEGTSQAWRTVKTQI
                     AQVAPSQAPVLIQGETGSGKDVVARLLHAHSRRAGGPFIAVNCGAVSRELLESELFGY
                     EKGAFTGAAQAKPGLIAAAEGGSLFLDEIGEMPGPMQVSLLRFLDRKEYRPVGSTRTL
                     RADVRIICATNRDIQELVLQGRFRDDLLYRINTVTLHVPPLRERPEDLAVLADHILHH
                     LRIPGTATRTLSPEALAHLATYRWPGNVRELRNVIERIVLMSPNSGPITSEEVLQVLP
                     RAASASSPDDRSHLPLDEIERLHIQLVLESSGGNKTKAAQTLRVDYKTLLAKLKKYEG
                     GG"
     gene            complement(71523..73109)
                     /locus_tag="PK224_02580"
     CDS             complement(71523..73109)
                     /locus_tag="PK224_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14652.1"
                     /translation="MRWLHDRSIGQKFFISFGVILSLLALSLTALLVYLSRINSYVDR
                     HKRITVPAIVTAATMQRSAYEMNLTLHLFLEQITKADAADTLARLSRHAEQIRSSLDL
                     YRSTHAARTHPILLGMLTQHQRMDLADQEDRAIARIDLILQELSALWQTALTGPQRAG
                     VAPSPVTKTDGLIVELMTNLDQLVAAHRDIDVEMKVEGDLLLQQARLIALSLVAVLGL
                     VITVIYVSVNRQIAKPLQRLSVTADRVAHHDLTAQFESWPGRDEVGTLAASLSSMVTS
                     LREQTAATARKTKELEAFTYSVAHDLKGPLREIEGFSSLLEKQFADGGDAQTRHQIDV
                     IRRSALRLTHMIDALLKYSRLEQQDLPRQRFNVLEMITTLITDRFSGLQGPKPKIQVA
                     LPFADLYGEPVSIRQAIANLLDNAAKFSRHASPPTITIGGTRMEHERILWVQDNGIGF
                     DPSHYDKIFGLFERLHSPQEYEGTGVGLAIVKLVMDKHDGRVWVESAPGAGSKFYLAF
                     PERAESVVDRPSSSPHPASA"
     gene            complement(73277..74302)
                     /locus_tag="PK224_02585"
     CDS             complement(73277..74302)
                     /locus_tag="PK224_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB14653.1"
                     /translation="MNIKTLNGLTALVSLLVILPAIAGAETRELLLAAYSVPKEAYEK
                     RIIPAFIRQWKQKTGETIQVRSSFAASGAQARAVVGGLEADIAALSLESDLQQLVKAG
                     LVTYDWKQGAQKGIVTTSIVALGVRKGNPKSIQGWEDLTKPGVEVLYPSPKTSGGAMW
                     DVITVYGAGLNQGKQKGLAGAAVDQHAADLVRRVQRNVKVMDKSGRESVTTFERGVGD
                     VIVTYENELLPRIKQGRPYELIFPAETVVVENPIAVIDRNADRHHVRDLADAFVSFLN
                     GEEAQQAFVEFGFRPANEAVARASASAFVHPPHVFTIEDLGGWDRVFAALFSPQGAWT
                     KAVEEAR"
     gene            complement(74345..74647)
                     /locus_tag="PK224_02590"
     CDS             complement(74345..74647)
                     /locus_tag="PK224_02590"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HRB14654.1"
                     /translation="MAILVVGGDSVDAITQRARAGGHGCVEHWSGRKTRDQTRSIRKD
                     TEAVVVILDRVNHTLARKVRIEATRRGLPVFFQKRGRQIRATGPRPLDLMRWLDRK"
     gene            complement(74678..75409)
                     /locus_tag="PK224_02595"
     CDS             complement(74678..75409)
                     /locus_tag="PK224_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14655.1"
                     /translation="MNGIRNTAQQTHPLSKRVMLALIGLTLVTGCAGRPVTPPKTIYQ
                     SGLSEVRVEQDPDSTANAHPAAVTATEVGTLLRGVRAWERRNVIHRLFVGEADRTRAF
                     RNEEITVLAPPLAKGLAQAGPTERVYFHLSQATDQGDEETTTGWISIRGPILHLTISG
                     VHDRHSPGPDISKYDRQLPNIPEASAAHDVTFEPEEFLVKVSSGSRLFAPDQREELQI
                     RYREALSSMPVQPGLERGSEKHSQP"
     gene            complement(75406..76230)
                     /gene="modA"
                     /locus_tag="PK224_02600"
     CDS             complement(75406..76230)
                     /gene="modA"
                     /locus_tag="PK224_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB14656.1"
                     /translation="MTRRNPFFGIWLFFSMLIGITGVVPSAQAQPETLTIAAANSLKD
                     ALRKVLPRFEAEHPAINVRVIYGPSQSLRKQIEEGAPVDIFLPSLFDEIDQLETKGLI
                     IQGTKRAFAGTSLVLITGAALPAPVRTIHDLETVPVRRIAIGDPKTSSVGKVAAQFLK
                     YSKLEPKLKSQYVFGEHSRAVLDLVAKGEAEVGVVYRTDAASNDKVRILDTAPVESHT
                     PVRYGVAAVWTAKNVSGAGDFIEFLLAPQTQALFQEYGFDRVTGDVGLVQRQEVKP"
     gene            complement(76267..77886)
                     /locus_tag="PK224_02605"
     CDS             complement(76267..77886)
                     /locus_tag="PK224_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14657.1"
                     /translation="MRAYTFFGIAVVAGSLIAGTITSVQAVEEGQLVKKDGKWEYYSG
                     EDPGLKYLFLKGIITQEEYDKGLRVLETREQIAKPNYSIDVNNGLNFRVGSKFFLKLR
                     LLTQVRYSHSAYNGGWGTVGDSRNPEILGGQVEYRAVSKQSDSNQFSVPRARLQFLGY
                     AFDPDFRYNISWAFDQTTWDQEGGSGRAGLLDAYISSWHIPWLTIQAGQQRVWFNRAT
                     ISSMATSTFADNLIVQNAFAANQQGSRDIGVSISSDEDQYKFNYAFGIWGGVGANLAR
                     EGTAVSQALPNNSSLPTAQRRTFNYDTRILTGEMMYTVRLLYKISGNPGYGQGDILNS
                     RTPQMAIAFGYAYNPAQNYLSSIRSDITDRAYRQRVTKAYNGRLLGGGIWDFQTYEAD
                     FIAKYQGWSFQAEGYYRHQRVRNADSGTIPFDPVTTTVLGPAVNLGQAYGWYAQVGKY
                     IIPRKLELAVRYGFMDPSTQQVNDLVKEFGAAINYSFDGTYNNRLVVDYSNITLGSGG
                     RAPDRFPFESQPGFGRDLIENRINVQYQFFF"
     gene            78113..79303
                     /locus_tag="PK224_02610"
     CDS             78113..79303
                     /locus_tag="PK224_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRB14658.1"
                     /translation="MKKAPAAEQSIQVENLLAPTRQAKGLSRIELASQAGITRQAVHA
                     IETNQYLPTTAVALRLADALGCRVEDLFRLLPQEDIVEGQWSDSATVQKTPQEAVRVK
                     VARVGARYIVRPVVDLGEVLNYAVPADGLLVPSRGVRTGAAASPRRVQVRLLRDRRVI
                     EQEIAVAGCDPSVFLAGDYLRRHQDSCTVVGWNLGSTAAIEALKRGEVHIAGVHVVDA
                     QSGESNLPYLRRHLKGNDYLVVTFAVWEEGLLAAAGNPKSVRGVEDLVRPEIRFINRE
                     AGAGARLLLDQKLIAAGMTPESVAGYATIARSHFQVARSIAEGHADVGLGVRAAAQLF
                     GLEFLPLQQARYDLVIPRVHINDHPSIESFLNILVSRPFRTEIDALGGYDMSQTGTVH
                     ALRS"
     gene            79300..80130
                     /gene="modA"
                     /locus_tag="PK224_02615"
     CDS             79300..80130
                     /gene="modA"
                     /locus_tag="PK224_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB14659.1"
                     /translation="MKRQYRSQPLLLCAAFLMGLLSLTPAPCVSAEPLVIAASPSLAT
                     PLEALARAYEAAHPDVQVKLYFDSGLDLRRMIAGMENSPTGQYFIGTGPIHLIAPGGD
                     ELITRLQQKYYVLPGSKRPYATVSLVMIVPESLVEAPVSFVAMARDSRMRVAVADPDM
                     TLLGQKTRDLFKALGIADVLNDRMDVATDARGVVDHVLNGQADVGIIFGPDAVEKADR
                     VRVVAVASEKEGRPILHSMAMERYCPNRTLCDDFLVYVQSAEAGTILKRLGYGVPTKP
                     "
     gene            80170..80820
                     /locus_tag="PK224_02620"
     CDS             80170..80820
                     /locus_tag="PK224_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14660.1"
                     /translation="MRVSSEHTGLLNLCMVVLGILVLGGCARLPYTKTVVHEDQRVAV
                     RLQAEVSSAGYSHPVEIPPPYIAAILGGLSLREQQRLPLRWFAEEVPPRRVFREDEVE
                     VLAPHLAAALQQVGPEERVYFEVFAPGVNPRYRRDVTGGWLAVREQYLHITMDYFHTQ
                     QPVEKSDAYDYNYPTPWTSPNTYLLYFEPGRFYLTDPKTNSRAVDFREFLKTLPPP"
     gene            80914..82671
                     /locus_tag="PK224_02625"
     CDS             80914..82671
                     /locus_tag="PK224_02625"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRB14661.1"
                     /translation="MASPVSLLIRSYSSFGLSGVGLGLLLCCLSLFHSTDAISAEAGR
                     LVEKDGRYVFVESMDPAMKLLLERAVKQGTITQEEYDGVVRASQERAYLLQPSFRAWY
                     DRGFNLSMNDNAFFLKIRARFATRYTQRYRNDAYRESGDSKNFPELLGVFGDYRANRS
                     IDQSSTFNLRIARLYFMGHLFSPDFRYYIQLAGETAENAQAPGALSVLDMNVTSTHLP
                     WMNVQVGQYKVYFNRSQINSTASMQFASRALVMDAFTASGINRRDVGLTIMNDEEVYP
                     INYYLGVFNGVGPLVNRFAQFNSEEATVGCPGGATGGNPFPSPAGCPTNTRNLNANLR
                     TNVNELMYVARLQANLMGRPGYGEGDLAYSESPQMAVGGGYAYNPSIDTSSNNAFVGI
                     DLANLHVRRSLAALGNGRMLGQGVVDFSTWTLDYVFKYRGFSLQGEYWYRNVTRHNKG
                     LPCMQTAVVGGPCTVFAPQEFGNSTGWTVQSGYYLIPRKVEVAARYAWWDPDTRSGGD
                     LIKQVDVSLNWFLSGTYDHQIMVTYSNIAMGQDGFAIGRSAPLPAVGATFPSGTVPLD
                     SRAGTLIENAVRIQYQIFF"
     gene            82684..83445
                     /gene="modA"
                     /locus_tag="PK224_02630"
     CDS             82684..83445
                     /gene="modA"
                     /locus_tag="PK224_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB14662.1"
                     /translation="MARLLLCVMFVLLCRPAIALADDIAVAAASDLQFAIKELIVEYE
                     KQTGHHVKLSLGSSGNFYAQLQQGAPFDLYFSADIGYPKKLEEAGLTVPGSLYRYAVG
                     RVVLWAPKQSPVEVSKGLTVLRDAAVRKIAIANPKHAPYGRAAVAAMEQSQVYAEVKD
                     RLVLGENISQAAQFIESGACDVGIIALSLAMAPVMKSAGSYWLIPAEAHPPLEQGAVI
                     MKQSKQQDAARHFLQFMQSPQGQEVMTRYGFTLPN"
     gene            83423..83785
                     /locus_tag="PK224_02635"
     CDS             83423..83785
                     /locus_tag="PK224_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14663.1"
                     /translation="MDSRCRISRGMPALALGAAVMIAGCSEAVLLTHETGQGGVVTYL
                     YKDERGGPMGSPYRREALKVMAEKCPAGYHVVKDGQVQSYSSGSSVEGAEGEEGTRRW
                     GIQFECKSGGDDRTTGRK"
     gene            83870..84073
                     /locus_tag="PK224_02640"
     CDS             83870..84073
                     /locus_tag="PK224_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRB14664.1"
                     /translation="MKLSARNQFQGQVVRITEGQAMAEVVVKVGNLEVVSAITEGSVK
                     SMGIKVGDAVTVAVKATDVIVGK"
     gene            84152..84826
                     /gene="modB"
                     /locus_tag="PK224_02645"
     CDS             84152..84826
                     /gene="modB"
                     /locus_tag="PK224_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRB14665.1"
                     /translation="MNWIAIWVTFKLAASTALVLMIVGLPIAYWVSFSRRRWKFLVES
                     VVALPLVLPPTVLGFYILVAIGPHSPLGRFYNELIGHPLPFTFEGLLLASILYSLPFA
                     VQPFAAAFEQVDRRLIEASWTLGVSKLGTFFKLIVPLSKAGLLTGAVLSFAHTMGEFG
                     VVLMVGGNLEGETRTVSIDIYDEVQALNYAGAAKTALFLLVVSYAILLLVYAVNRNVW
                     AAWPRK"
     gene            84811..85905
                     /locus_tag="PK224_02650"
     CDS             84811..85905
                     /locus_tag="PK224_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB14666.1"
                     /translation="MAAEIDIQLVKTFPGRAPIKARIRYPVEASTVLILFGPSGSGKT
                     TILRSVAGLEWPEEGTIQFVSRTWLDTASGVRVAPQDRHIGYMAQDYALFPTYTVAGN
                     IGYGLHHLSAQDRNKRVDEMVELFQLRGLETAKPRELSGGQQQRVALARAVAPRPLLL
                     LLDEPLSALDAPTRVHLRDELRSLLKQLALPSIIVTHDWTEALTLGDVMAVISAGNVL
                     QVGPPIEVFSRPHNADVARVVGIETVVKGRVVGSSDGMVQVSVNGTRLTAVEGESAGP
                     DVFVCIRSEDVVLERGQATASSARNHLAGTVISATVLGALAQVTIDCGFPLVAMVTRS
                     TVEEFGLSSGVPVVAAIKAGSVHLVSRQSD"
     gene            complement(86061..86810)
                     /gene="traT"
                     /locus_tag="PK224_02655"
     CDS             complement(86061..86810)
                     /gene="traT"
                     /locus_tag="PK224_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HRB14667.1"
                     /translation="MTRQVTRHLSPCIAGGWLVALLLLTGCSNILRSGLVNDTSILLP
                     PSTARTIYVQIRNTSENQHATPADIPARLMAKGYQVVNDPLGAAYWLQTQVVYCHKAG
                     DGVRPEMVAKAGFGAGIGSGGTPLANAGGLDMDAMGGMFGGSGGGGVNMQALRGMAMG
                     GGAAPDLNAMMRMAMSGRGGYPGMAPPEQNHDSLYLCVADVLVTEQGKTDQPRRYKMR
                     SVAHVLPKKLNLEEATPIVREKFGASITGAF"
     gene            complement(86821..87540)
                     /gene="traT"
                     /locus_tag="PK224_02660"
     CDS             complement(86821..87540)
                     /gene="traT"
                     /locus_tag="PK224_02660"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HRB14668.1"
                     /translation="MTAGLFRSLLAGILLSTGCAANVRDTDLLASNSLMLPPSTARTV
                     FVQNRNASDNQAVSLHDLRARLNLKGYEVVQNPQAAAYVVLTNIVYCNQTKVELPVED
                     LVAGGYGSGIGSSIMSGLHGLTGMAGMAGPQGALIGGAAGMGLDAAEGLGNAVGNMFG
                     GPSRPDANDNINYACVADLQITELARTGGTLPVSPPGTTPPAGVYQTRLAASVYQKKL
                     DEQEATPLVQQRLSAAVAGHF"
     gene            88053..88604
                     /gene="lexA"
                     /locus_tag="PK224_02665"
     CDS             88053..88604
                     /gene="lexA"
                     /locus_tag="PK224_02665"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRB14669.1"
                     /translation="MMTSSEFRQIREQLGLTQEQLAAALRTTRVSVARYESGMRRISG
                     AVQVAITQLANRTQIPMAGVVAAGLPIEPVTQSEWVEVPPGMVRRGETFALRVKGESM
                     IEDGILPGDLVVVRKQATAHNGQTVVALVNREATIKTYFKHARHIELHPANAAMKPFV
                     VTPEDEFSIEGVLVGVIRHCENA"
     gene            88619..88870
                     /locus_tag="PK224_02670"
     CDS             88619..88870
                     /locus_tag="PK224_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14670.1"
                     /translation="MMMSHQQQAMVERVDELERMIVRLNGRIGELQRELKHTKGMTFQ
                     GWSGGSRLRTLLGGMKPVLSASHPIRENRRIERHDLCLF"
     gene            90143..91678
                     /locus_tag="PK224_02675"
     CDS             90143..91678
                     /locus_tag="PK224_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB14671.1"
                     /translation="MAKEPLQVLVLVGAMVMISGCMIKPYVLTDEDVRTRATNDLQMV
                     STLREPLSGPIDLYEAIARALKFNLDAKVKAMQAQLAHQQLNVAHYTLLPQLSANAGF
                     DGRNSYSGGGAQSLVDGRPVLEPFTSSDKNVVSGNLALSWDVLDFGLSYVRAQQAADN
                     VMIAEEERRRIAVRLVQDVRSAYWRAVSAERVLYRVKFLDESVNKALERAQQIVDKKL
                     QTPLTPLNYRRDLLNIQREVQRLYRELSTARVQLASMMGLPPGTEYDLVVPQRASTVP
                     VVDLDTGRMEEQALLLRAELRSLDYQKRINAKEARAVFLEMFPNLKLSLGGYYSSNSF
                     FLYQNWLGYASQLSWNLLSAFRVPAKLKAVEAHRQVLDAQSLALTMTILTEVHVGALQ
                     FAQASQEYLNARSYHETQLAITDHTKNLWLTKSTNDLTFIRERVNDVLAEVRMDSAQS
                     GVESAYATLMASMGEDAAPTGTDGQSVAALADELRKQRVPAVDTVSESGRRLESHAIP
                     LAN"
     gene            91656..92441
                     /locus_tag="PK224_02680"
     CDS             91656..92441
                     /locus_tag="PK224_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB14672.1"
                     /translation="MRSHWLINSMVALTIFCAAVAFPQVGWPKDDRPVQLMRSVNGAL
                     RGIVKASTQAVLYSQIQGRISQIPVKEGQRFEKGATLVAIDCDKYRAELAGALAEHEA
                     KDKTFQNNKELTKLNAVSTLELEISEAEAKKAVAAIRLAEINVRSCHIAAPFSGRVVA
                     VMVNEHENVFPNDKLLSLLDDTSLEIELVLPSSTLAWLKRKSPFTFTIDETHREYPAK
                     VKEIGANVDPASQTVKVVGSFGALPADVLAGMSGTAQFASGQP"
     gene            92446..93810
                     /locus_tag="PK224_02685"
     CDS             92446..93810
                     /locus_tag="PK224_02685"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRB14673.1"
                     /translation="MATSTQPSAQELATLVHLATLTHLEAQIRVAKTVQELQFISVNE
                     TRRLVPYEQAYLLSHAGEAAGVSRVICASSVAVVERDAPMIRWLEEMAQALRHAHPGD
                     DPVTVTEEMCPDSCREGWREHVRGHVLWCPLKHPDETVLGFWWLERDYPWEENDRAIM
                     HRLAGSFAYAWKAVATRHRTWSVRIKTPSVWLMAAAVVGALCLPVRVSTVAPVKVMAK
                     EPTIVSAPMDGVIAEVLVLPNTVVTQHRPLFRYEDTNLRNQFRVAEKQLAVAQAEHAQ
                     AIQGGFADPQRKAEVPLRAAEAALKETELTYAQEMLAQVEVRAPQEGLLLYSDKADWV
                     GKPVSVGERIMDIADPRRIELRVDLPVSDALLLREGADVVAFFDALPLESFGATVSRA
                     SYHAEVLLGDMLAYRITAELATADPRIRIGWQGSAKVYGEQGPLAFLLFRRPLMALRQ
                     LVGV"
     gene            93814..95952
                     /locus_tag="PK224_02690"
     CDS             93814..95952
                     /locus_tag="PK224_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRB14674.1"
                     /translation="MAGLSSVSPPQERTLPPLRKNLQFLRGAPTPEGVPTWTIVDPVR
                     NKYFQIEWQVYQLLQRWSCGTVEKLVAVVRRDTTSRIRAEDVEDLVRFLYANSLTEQS
                     ASGRVKDYVEQEVARHQAWWQWLLHHYLFIKIPLIHPHRFLHATLPLVAPLYTPMAAW
                     LFGMIGATGLFLVGQQWDTFLSTFLHFFTWSGALMYGAVFCAVKVVHELGHAYTATRF
                     GCRVPTIGVALMVMMPVLYSDISDAYRLSSRRKRLWIAGAGVVAELGLAAVATLVWGF
                     LPDGAIRSVVFVAATTSWVMSLAVNLNPFMRFDGYYLLADGLGIPNLQDRAFAFGQWR
                     IRELLFAPGAPPPEAVGLSNRRILIAYAWGIWLYRLVLFTGIALAVYHYFFKVLGLLL
                     FLVEIVFFIGLPIWRELTSWWSRRAAYASTGRFAVTMTVVAAMLLLACLPWSSRVAIP
                     GVLQAGSYATIYPPAPGRIASVSVHAGQSVRAGDTVLVLENPLLAKEARLARTQVELL
                     DFRLQRQAGYAEDRLQGQVLAESLRSKLVELDGVAEKQQRLVLQAPIDGVVVDQADSL
                     YPGRWINEKLAVAYVVNSRTAIVTALAPVEDLGVLAVGQEALFIPNDVTRQATRARVT
                     EIRDVDEQDLSVPYLASIYGGAVPVRKDSHGRLQAEQSVYRVELEVLDEVVRADQVVT
                     GLIHVARTPQSLVARMWDRVVAVMIRESGL"
     gene            96031..>98479
                     /locus_tag="PK224_02695"
     CDS             96031..>98479
                     /locus_tag="PK224_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HRB14675.1"
                     /translation="MFDFIKKYLRRNGEPSERPSSLATPSAFKQSLLSLEQRLMFDAA
                     AAATAAEVQSEQVAQDQAEAAVSSEGTDESTTPEQQESQELLHAIGSYSPGESSTEVV
                     FVDPTVPDYESLLAGMGPNVEVVVLDASRDGVEQIAESLTGRSGIDAIHLISHGDAGT
                     LQVGTGTLTAESMSTRYVDELATVQQALSEQADILVYGCNFAEGDVGQAAVARLAELT
                     GADVAANSDRTGHIDLGGDWKFETQVGSIETDLAITDAAQMDWKGILTTETVGDQFSL
                     SSYDNHDGTQKWASDWSETDAGGGGASGGDVHVASSQLRIDAGSVGNQASRQVDLSGT
                     SGAILTFDYTNSLSGSDQIEIRVSADGKTYTTLTDGVFSPKSNAGSGTVKLDISGYAS
                     ASTTIQFLVTGVGGGDSLSVDNVQVSYDTGAANSAPTISSNGGGTTASVTVGENSTVV
                     TTVTATDVDAGHTLGYSIAGGADAGRFTINNSTGQLSFASAPSYEAPADSGGNNVYDV
                     TVQVSDGQGGTDTQALAVTVANVNEAPTDLALSANTVAENAANGTVVGTVSGTDSDTG
                     DTRTYSLTDNAGGRFAINSSTGQITVANRSLLNYESVTSHNLTVRVTDSGGLTYDETF
                     TINVTDVNEAAPRITSNGGGATALINIAENATAVTRVTASDADTRQTLAYSIVGGADA
                     AKFTINSSTGALSFVSAPNYEAPTDSGGNNVYDVTVQVSDGSGGVDSQAISVTVTAVN
                     ERPTDLSLSANTVAEHAANGTVVGTVTGSDPDAGDTKSYSLTNTAGGRFAINRTTGAL
                     TVANSTLLNYEAATSHAV"
     assembly_gap    98480..98579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <98580..100579
                     /locus_tag="PK224_02700"
     CDS             <98580..100579
                     /locus_tag="PK224_02700"
                     /inference="COORDINATES: protein motif:HMM:NF027510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HRB14676.1"
                     /translation="DPTPNTLTVNVTAVNDRPTDLSLSANMVAENAANGTVVGTVSGT
                     DPDAGDTKSYSLTNTAGGRFAINRTTGALTVANSTLLNYEAATSHAVTVRVTDRGGLT
                     YDETFTINLTNVNEAPSGTNATVTITEDTAHVLTAANFGFSDVDAGDALSAVRIDTLP
                     TAGTLTLSGTAVTAGQVITTADLAASQLVFTPAANANGTGYARVAFSVRDSTSLYDPT
                     PNTLTVNVTAVNDRPVMTANSGSSVAEGGTDTITSAELAAVDVDNSAAQIRFSVGTGP
                     AHGRLELTTRPGVALATFTQADLAANRLVYRHDGSETLSDRFTFTVSDGAGGSVGTTT
                     MTLTITPVNDAPTIINNEGAATASINVAEHVTGITVVTGADVDLPVQALTYSISGGAD
                     QAQFTIDASTGALSFVAPPDFEAAADANGDNVYIVQVRVTDSQGGTAMQTIQVRVTDG
                     AEARPSGPALPPSLIPMPSVQAGTSVQPHDEPLSFTTRQEPVNAVSMNGTDLAGTMSP
                     YAGSMPPRVAPHSGDVHHEEEPPVASRSVQSDDREPRNSSPISSTDAIEAGRDGDSGP
                     QPSASELVLNKLDEMILSLREAVAVDREPQSLMAQVTALTGATLSVGFVLWAVRKGRL
                     LAGCCATIPTLSAEGPGLGQHLRPRLLTKPSPQSQGPVNIP"
     gene            complement(100735..101457)
                     /locus_tag="PK224_02705"
     CDS             complement(100735..101457)
                     /locus_tag="PK224_02705"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB14677.1"
                     /translation="MNDWKAEWETLQGQASQIERLIAERAHNIFEKSGRHHGNSLEHW
                     LQAEKEVLNFLLSVVHSQLFEQQSKWVRISHKLANLFPRNAPEQMGCVMTIQKLARFE
                     LEIVKKDTAFWINRLRQIPEQPLELDDILTHSYLWVLGAYEILRTLAAATGHPRLIQA
                     KRMFARIRMPLAKLKASEKYSEKDFNFPYPITKGPCIGWAVNERDIVLRDALSSEFLK
                     ALDEFCESLGMAAEIRGDRQES"
     gene            complement(101820..102989)
                     /locus_tag="PK224_02710"
     CDS             complement(101820..102989)
                     /locus_tag="PK224_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14678.1"
                     /translation="MLIRPRQGNAWTRKLLLPGMMMLATVLGGQARAAQFSFPPFPGD
                     QNACYQYLSTNISTSPGTYGTDLRSHYDELTDSSIIATLQGNLRALSPPPTCTAANWT
                     PVVGQIKTELSYVQTANDWLLGPTGSQTLLTQIFAETDFDVDSVSAKLQNVDPADQIA
                     ANLTQLFLGVGLAIGGSVVGLPPVFSNVFNQVTNYIGKHGGFPSDPNLAVGEVKGKLA
                     IMYNTALNGNNDTHDQLVTDWSQLKAFAAPIVGTAPTDEQQKAMRNAGEMSYAMWLWQ
                     TLSPTRWHIVVPLIRNDLRGCYFQMVGGYPNPAINYPVSNNFDCGPAWIGDKCYDTQC
                     NSPSTEAFDFLFKDCPTGQAECPDPANGPLKINPADVFLSRNGWSLPCVGTCPLQ"
     gene            complement(103409..103484)
                     /locus_tag="PK224_02715"
     tRNA            complement(103409..103484)
                     /locus_tag="PK224_02715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103449..103451),aa:Met,seq:cat)
     gene            103905..104834
                     /locus_tag="PK224_02720"
     CDS             103905..104834
                     /locus_tag="PK224_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRB14679.1"
                     /translation="MLTGVSLQTALPWLLSISTTVGIGVLRVGLVFVIGYIAIRFARL
                     GLQQLERVMLLTSDAKDQQSGTAQKRAATLAGILRTIALTAIWAIVIIESLQVVGLDI
                     APILAGAGILGLAVGFGAQNLVRDLISGFFIILEDQIRLGDVAVINGTGGQVETITFR
                     TISLRDFSGVVHVFPNGGINTLSNMSKDWSAFVLDMGVAYKEDTDRVVAVMQAVGEEL
                     RHDEELGSLMIEPIEVVGVENFADSAVTIRARIKTQPLEQWKVGREYRRRLKKAFDAQ
                     GIEIPFPHQTLCMGEASPPFKVEVVAGLGLQKV"
     gene            complement(104936..105106)
                     /locus_tag="PK224_02725"
     CDS             complement(104936..105106)
                     /locus_tag="PK224_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14680.1"
                     /translation="MVTIIGLVTIVTLIWLLAWAMGLESDSERRKLISASSAASPTHS
                     DIVPERQTRRAA"
     gene            complement(105223..107133)
                     /locus_tag="PK224_02730"
     CDS             complement(105223..107133)
                     /locus_tag="PK224_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRB14681.1"
                     /translation="MSTLFTGFERIEEARERFTGKSFLAGLYDGRPDFNLLLTPPEPP
                     EEKTAGEAFCKQVEHFLIHEVDPQEIEREAKIPERVIQGLFKLGAFGMKIPKEYGGLG
                     LSYTNYGRVLTLIASWSNILALTVAVPQSIGIAMPILLFGNADQKKKYLPLVAKEALS
                     AFALTEPMTGSDAANVRTEAVLDSSGTHFLVNGEKLWCTNGPIARYLTLVARVPALRV
                     ECEGKVEWIPAPQGQRADDHVHTAFILDMATPGVLVRQRCQFEGCRGIENAHIALKNV
                     QVPIEHVIGDIGKGLKYALTILNVGRGISIPAICLGMAKQAWQPTLDRANSRVTFQKP
                     LAERQTQQIRIGDMAGHLFAMEALALLVWRLADQHRYDIRIEAAVAKIFCSEHTIRFI
                     RDAQTIFGGMGYETADSKHARGEAAFGIEQLVRDAEMYRIGEGATDILRPFVVREGLS
                     PHLDRAKRFYADGLSILEQARQAMTLMRFYLPWYLRQWRKRPLPDRREITHPQVRPAA
                     LYVERTSRRLARAIFYALLRFQASFKDEQRLQNKIESVGEDLLAMLATVLYAERQTRL
                     DGHTSVWELAEAFSAAAKQRIEQRLREFRHHRDTTAATTGRQALKGYYPTLSEGIIRR
                     RLEDYRRNQHMT"
     gene            complement(107155..108876)
                     /locus_tag="PK224_02735"
     CDS             complement(107155..108876)
                     /locus_tag="PK224_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRB14682.1"
                     /translation="MSLLTDVHDRIEQAREAPGCASELHWTSFAEFFRSRLYDPRLVT
                     RNFLTYCDDDRNLRRTYSYAEFGTIVERMAEMFHAKFGLTRGDRIATVLFNHDLTALI
                     YFAAWTLGVAVVPINVEETPEKKRYILEHSEASVALCWQDVYEELNALQSMLPALREV
                     VALGDGGILDGKETRGKSRKNSHAVGARFAPGTSRLDDPALIIYTSGTTGPPKGVILT
                     VANLLIDADAIADRHGFGLTDRLMCVLPIHHVNGIVVTLMTPFYCKGSVVLNRKFKTG
                     SFWRRLHDEQATSVSVVPTLLEFLLDADEDLAAYRLDRFGGVICGAGPLLKDTALRFE
                     ERFGFPIRHGYGLSETTCYSCFLPNDLTGEEHRHWLTDFDFPSIGTALRHNEMAILDE
                     SGKAVAEQARGEICIRGRTVCAGYFKRDDANEAAFQWGWFRSGDEGFCIADDRGRPFF
                     FISGRLKELIVRGGVNISPLEIDDVLKGHPAVQFAMALPFENRYYGEEIAAYIVPKDP
                     ASPPTEVDIRDHCRGRLPFAKQPKVLVMGTEVPYTSTGKPKRLELKSRLAGTLAQYRD
                     RQFKDPA"
     gene            complement(108851..110029)
                     /locus_tag="PK224_02740"
     CDS             complement(108851..110029)
                     /locus_tag="PK224_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRB14683.1"
                     /translation="MNQAIQEQPGTKTTERPLYSPGLEGVIAGESALCQVDEGEAGLR
                     YRGYAIGDLAEWSRFEEVAYLLLIGHLPTRNELDGFSTLLRGHRVLPELVQRFVDRLQ
                     PGLHPMDVLRTGISLLGLSDPDAQDGSHEANLRKSMRLLSQIPLLIAHSYRVMSGILP
                     PRQDESRDFAQHLLHLVAGHKDGETGTAMAQALNVSLILYAEHEFNASTFAARVTAST
                     LTDLHGAITAAVATLKGPLHGGANEAVASMLIEIGSPERAETWVREALAQKRRVMGFG
                     HRVLRQGDARSAIIQRHAERLSHLCGDHSWYDIASTLHRIMREEKGLHPNLDFYTAVA
                     YLLMGIPRELSTPLFVCSRITGWCAHVMEQQEHNRLIRPRALYTGPAPRTYEPLDRRS
                     "
     gene            complement(109971..110912)
                     /locus_tag="PK224_02745"
     CDS             complement(109971..110912)
                     /locus_tag="PK224_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRB14684.1"
                     /translation="MRNHSTPSKAQRLRGLLAGRTVAIPGACNALTAMQIERAGFEIV
                     YVSGAAISAAHGLPDIGLISLTDMTREASAIARSVALPTIVDADTGYGPPSVVREAVQ
                     EFERAGLAGMQIEDQEEAKKCGHLSGKRLIPTADMVAKITAAVEAKCDRDFVLVARTD
                     ARTVDGLQAAIQRGVAYANAGADALFPEALLSADEFHTFALEMNRAGVHLPLIANMTE
                     FGKTPYLSVDEFETLGYRGVLFPVSTLRVAALAVEKLLRELRFFGSQRAWLDHMMTRQ
                     ELYSLLRYEHGQNQTGRPYEPGDTRTARNENHGAPPL"
     gene            complement(110905..111309)
                     /locus_tag="PK224_02750"
     CDS             complement(110905..111309)
                     /locus_tag="PK224_02750"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14685.1"
                     /translation="MFPYCVAVALLDGRVTLHSFDPHRLRDAALHELMKKVRVVPQPE
                     FAGRYPGAMPTRLTLRTTTGTVYMRHVDVPLGHPGNPMSDQDVEDKLRRLASRRIGLA
                     QVEKLIGFVWTLEREKDIATLMPLLRVPHRDA"
     gene            complement(111331..112308)
                     /locus_tag="PK224_02755"
     CDS             complement(111331..112308)
                     /locus_tag="PK224_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRB14686.1"
                     /translation="MLADRLASYTQALCYDDLPGDVVHEVKRRILDSAACAFGAWKAT
                     PCRIARHVAQSAKVPGGATIWGTSHKTLPDLAAFANGALVRYLDFNDTYLSKEPAHPS
                     DNLAAILAAGEIAHASGKRVIQAIALAYEIQCRLCDAAALRSRGWDHVTYGSISSALG
                     AAKTLKLTEAQTTQAINLAAVANVALRQTRVGEMSMWKACAFSNAARNGLFAAILAQR
                     GMTGPAPIFEGEKGFMKLVSGPFELSRLGGERAPDDTLASFKILDSYIKHFPVEYHAQ
                     TAVEAALALRAEVIKAEGADAIQRLSEIEIGSYDVAIEIIGRDPEKWHP"
     gene            complement(112338..113060)
                     /gene="meaB"
                     /locus_tag="PK224_02760"
     CDS             complement(112338..113060)
                     /gene="meaB"
                     /locus_tag="PK224_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRB14687.1"
                     /translation="MARQVLAGGVRAVARVITWIENQEPVGAAVLKRLTPPEKRAMVI
                     GITGYPGTGKSTLIDQLIAAYRRLGKTVGVLAVDISSPLTGGAVLGDRIRMQRHADDA
                     GVYIRSMATRGQSGGLARATRDALQVLEAAAYDVILIETVGVGQSDIEVVSVAGTVIA
                     LVAPGLGDDVQAMKAGLLEVADIVVVNKGDREGADTAVHDLREWCPHVLRTVAVKGEG
                     IPELIAMIAEQQRLQDLAHTLG"
     gene            complement(113113..114060)
                     /locus_tag="PK224_02765"
     CDS             complement(113113..114060)
                     /locus_tag="PK224_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRB14688.1"
                     /translation="MLQAGLHNSRQAFVRITEVSPRDGLQHESEFVPTLRKIALINAL
                     SRTGVTEIEAGSFVSPAAIPQLADSDEVLRAIDRIPGVAYSALVPNERGLERARAAGV
                     DKIAVFTAASNSFTRHNIKATVQESLVRFRPIVAAAKRDGMAVRAYLSTVVFCPYEGR
                     IAPTQVRDVMERLLDLAVDDISLGETVGKASPTDIRRLLDTVLPHVEPARLSLHFHDT
                     YGMAVANALTAWWDYGITAFDASAGGLGGCPYAPGASGNVATEDLVFALKTSGANVPV
                     DELLVAACAQQLGDAVRHPLNSRLSQLHHREKPQPAWRE"
     gene            complement(114038..114886)
                     /locus_tag="PK224_02770"
     CDS             complement(114038..114886)
                     /locus_tag="PK224_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRB14689.1"
                     /translation="MRRYTTLAMESRGDIRCITLDRPERRNAFDSRMISELTQAFDES
                     AQEPSLRGVLLSSAGPVFCAGADLQWMQSTSPISEAQAQDDAHRLTRMFRAIDECPCP
                     VIARVQGPAFGGGLGLMAVCDIVVAAEDAMFSLSEARLGLIPAVIAPFLLRKAGESFL
                     RRYALTGETFTTSVAHRFGLVHDVVPQSDLDDRITEVIDAIRQLAPRATRATKGLILR
                     IRPLPDQERVVTCAEANASARGAAEAIEGLRAFAEKRPPQWAKGREQQPARERAKEHH
                     VASRPT"
     gene            complement(114913..116517)
                     /locus_tag="PK224_02775"
     CDS             complement(114913..116517)
                     /locus_tag="PK224_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRB14690.1"
                     /translation="MRVLKSTVNKTSEDFRRAQVQYESLTADLKKHLAIVQAGGPADA
                     VALHKKRGKMTARERIAALLDRGSPWIELSPLAAVGLYDNQVPAAGLITGIGSVSGRF
                     CVIAANDATVKGGTYFPMTIKKHLRAQEIALENRLPAIYLVDSGGIFLPMQAEVFADK
                     DHFGRIFFNQARMSAAGIAQIAVVMGMCTAGGAYVPAMCDENVIVQGTGTIYLAGPPL
                     VKAATGEDVTSEELGGADLHTRLSGVSDHLAQDDHDALDICRSIIATLGRRPPRIRRE
                     KIDEPLYPAEELYGIIPANPRQKWEVREVIARLVDGSRFQEFKAGYGATLVCGFAHWM
                     GHLVGIVANNGVLLSESALKGAHFVQLCAQRRIPLVFLQNITGFMVGKDYETRGIIKD
                     GAKMVQAVATAEVPKFTILIGASHGAGNYAMCGRGYGPRFLFTWPNARTSVMGAQQAA
                     QVLVTVKQQQRAREQGRLSDEEQRRITEATRSQYEQEGSPYFSSARLWDDGILDPVET
                     RKVLGLCLDLAASVPARETHVPVFRM"
     gene            complement(116531..116938)
                     /locus_tag="PK224_02780"
     CDS             complement(116531..116938)
                     /locus_tag="PK224_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRB14691.1"
                     /translation="MAIATTPIRVLIGKVGLDGHDRGVKLVVRALRDAGVEIIYTGLH
                     QTPEQIVTTAIQEDVQAIGLSIHSGAHNSLFPRVLQLLKEQGAGDVTLFGGGIIPDDD
                     VPRLKAAGVQMLFRPATPMHDIVDFVKGLRVER"
BASE COUNT        22827 a        32547 c        36277 g        25235 t
ORIGIN      
        1 gcgcgtcggg ttatcagttt gtgtcgcagg aagaggtgtt ccggctggcg gagccggtgc
       61 cggacggggc gaccacgaaa cggcttgtgg aaattgccgc acgccgtggg atgaccatcg
      121 tagccgggtt gccggagcgg gacggccggc actgctttaa ttcggcggtc gtggtcggac
      181 ccaggggatt catcggctgc tatcggaaaa cgcatctctt tttcgaggag acgctctggt
      241 ttactccggg cgacagcggg tttcacgtgt gggatatcgg catggccaaa gtcggcgtca
      301 tgatctgctt cgactggtat tatccggaat ccgcccgcac gctggcgttg cagggcgcag
      361 atatcatcgc gcatccgtcg aacctggtgc tgccacattg tcctgattcc atggtgaccc
      421 gctgtttgga aaatcgagtc ttcagcgtga cggcgaatcg gatcggtagc gaagcgcgcg
      481 gaggaaagga ccggctgacg tttatcggca tgagcgaagt cgtcagtccg cgcggccgga
      541 tcctgcatcg agcaccgcgt gaaaccgagg acctgacgat cgtcgagatc gaccccgccg
      601 aggccaggat caaagcactc aatgcctaca acgatctcct gcgcgaccgg cgcccagcct
      661 tgtatggcga ctaatcccgc gtcgtatacg tttcatgact caacttgcgt cacttacgag
      721 caccaggtag gatgaaccat gtcaattcca ctcggtaaag gcattctcct cgtggccgcg
      781 ccggcgttga acgatcccaa cttccggcag acggtggtgc tcctgtgtga gcatgggccg
      841 gaaggggcgc tgggtgtgat cgtcaaccgt ccgaccgcca tgtccatctc tgaggcgctt
      901 ccgcaggtgc cgatcctgga aggccaaccg catgtgttgt attccggcgg ccccgttcaa
      961 accaatcagg tgatgatgct ctatcgcatc aatcagacgc ccgaaaattc gcaccaggtg
     1021 tttgacggtg tctgtctggg cggcgactta gagattatgg aacggatcct catggagcag
     1081 cccgggcagg aatcgtttcg agcgtacctg gggtattcgg gctgggggcc cggccagctg
     1141 gagtcggaaa tgcaggttgg ttcctggatc acgttgcctg ccgatccctc gattgtgttt
     1201 gaaaaagaac cgacccgcat ttggtcggat atcttcctct cgctggacga cacctcccgc
     1261 cattatgcgg atatgccgtt cgatccctcc tccaactagg cagcccccat tatcatggcg
     1321 ggttctactg caaccgcagc tacgccgctg cgacaagcct ggtcgccaat tgttcgcgac
     1381 caggcgggcg ggctcgccgt gcggtcagtc aacatccagt tcaggatgcc tccttccctt
     1441 acggctccac gcgcccgtct cgcttggcgt ttctgcgatt tcgtaacgaa ccgccacgaa
     1501 taacggggac tacctagttg aagatagtct caatcgttaa gcgtcgttgt gtctcgtacg
     1561 accttgacga tcgagctatt gcaccatggc atcggacgag atacgcttca cgtttcacga
     1621 ggtgcggggg gctccgccca acaccttcgc gagaaatttc cccgtatgag actgtgtgac
     1681 tttcgcgacc tgttcggggc gtccttcggc cacgatctgt ccgcctgctt cgcctccttc
     1741 cggtccgagg tcgatgaccc aatcggcggt cttgatgaca tccagattgt gttcgacgac
     1801 gatgagggta ttcccggcgt ctactagctt gtgcagaacg gtcagcagct tcttgatgtc
     1861 gtccaggtgc aggccggtgg tcggctcatc gaggatgtag agcaggttgt gggcggacgg
     1921 atctttcagt tcggcggcga tcttcagccg ttgggcttcc ccgcctgaca gcgtggtggc
     1981 cgcttggccc agccgcagat agcccaatcc aatcgaggac agcaggtaga gtttttcggt
     2041 cagtttcggc gatcccgaga agaaggcctg cgcatctgtc acggtgaggt tcagcacatc
     2101 gtggatcgtt ttaccccggt accggatgct cagcacctct ggcttgaagc gcttgccatc
     2161 gcactgttcg caggtggcgt agatgtcttc gaagaaatac atctccagct tttcgtaccc
     2221 gtttccttcg cagcgctcgc agcgtccatc ggcggaattg aaggagaaat gcccgggtgt
     2281 caggcctcgg cgcagcgcct cccgctcgga ggcgaacaga gtgcggacct cgtcgaatgc
     2341 cttcaggtag gtgatggggt tggagcgcgg cgtccggccg atcggttgtt ggtcgatgag
     2401 ccgcacgcct ttcacatgtt cgagtccctt gatggcctga aactttccca tcgggagcga
     2461 ctcgatgcga aaggcgcggg cggcggcacg gtacagcgtc ttttcgatga gggtgctttt
     2521 gcccgatccc gagacgccgg tgacgcagac cagcatgtgg agcggaatgc ggaccacgag
     2581 attcttgagg ttgtgctcgg ccgcgccggc gatgctcagg accttgccgt tgcccgagcg
     2641 tcggatcttc ggcaggggaa tgcggttctc cccgcggagg taacgggcgg tgagtgaggc
     2701 ctgatcggcg aggaattgtt cgcgtggcgc cgcgcagacg atgtgcccac cctgctcgcc
     2761 tgaaccgggt cccatttcga cgatgtggtc ggcggcctga atcatcgacg gatcgtgttc
     2821 caccacgacg acggtgttgc catgattggc gagatcgcga aggattccgg ccagggtgtc
     2881 cgtgtcgcgg gcatgcaggc cgatcgtcgg ctcatccaat acgtagagcg tgcccactaa
     2941 gcgcgagccc agctggttgg ccagtgcaat ccgttgtgct tctccaccgg acaaggtttt
     3001 ggtctgccgt gacagggtga ggtagctcaa tcctacgcgc agcagaaagt tcagcttcgc
     3061 gtgcagttgg cgcagaatat ccttggcgac ctccgcgtcg aaggcgggca gggctaggcg
     3121 ctcaaaccag gcggctgccg cttcaatggt gagctcaccg attcccacga tgtcctgtcc
     3181 tgccagtttg acgagccggg cggccggctt caggcggctg ccgtgacagg tcgggcaggt
     3241 ggcgggactg cggtagcggc tgagcagcac ccgcacatgc agcttgtagc gtttggtttc
     3301 cagatagtcg aagtattgcc ggactccttc aacttgatcg ctcccttccc agatcagctg
     3361 ttgcacctct tcgggaagct cttgaaacgg ggtcgtcaaa tccacgccct gtttcttcat
     3421 cgccagcaat atttgcttct gccaccagtc cgagcccggc ttgctccagg gctcgatgac
     3481 gccgttggcg agcgatttgc tgcggtcggg gatgaccaga tccttgtcgt attgcaggat
     3541 attgccgaac cctttgcact ccgggcaggc tccgagtggg tgattgaagg agaacagcag
     3601 cgggcgtagt ggttcgaagg tccgcccgca cccctggcag cgaaagtggg tgctgtaggt
     3661 cctgagcccc tgcccgatga ccaatacctg acaggtgcct tctgcttcct ggaacgccgt
     3721 ttcgatggcc tcgatcaacc ggtgccggtt gtccggtctg aggacgagtc gatccagcac
     3781 gacctggatg cccgattcgc gggtcaccgg gagcacggcc tgctcgtgaa gatccaggag
     3841 ctcctcaccg catcgcagcc gggtgaatcc ccgtttcaaa agtgagtcga gcagcgagcg
     3901 gtcgtggcca gggccgagat ccttgagggg aaaaagaacc atgacgcgcg cgtcggggaa
     3961 tcgtgccagc agttcctcgg cgaccgaccc cggatggtaa ccacgcgcct cctgtttgca
     4021 atccgggcag acgggcttgc cgattttggc gaagagcagc cgcagcaggt cggcgagctc
     4081 ggtggcggtg ccgacggtcg aacgggcggt gcggatcgga tttttctgtt cgattgcgat
     4141 cgcggggcgg atgttggtga tccgatcgac gtcgggccgg ttcaccttgt cgagaaacat
     4201 ccgtgcgtag gtcgagagcg attccacata gcgccattgt ccttccgcga acagtgtgtc
     4261 gaaggccagg gacgatttgc cggatccgga cagcccggtg atggcggtga cccggttgtg
     4321 agggatccgc agggagatgt tcttgagatt gttctggcgc gcgccttcga cgatcaagtc
     4381 gttcgtggtg caatctggcg gactacggtg tgccacggca gcggttcctt ccggagaaag
     4441 accgaccatc gtagcaagtg ccgcagtcgt ctgcaatgtc acccgtcggc gtcggcgctg
     4501 agatccgtgt gatccgtccg ggcagattcg tataatggct ctctcatcgg gaggactcta
     4561 tggttgatcg atcggcctat ctcgcgcgca tcggatatca aggctcggtg gttccgtcga
     4621 tggagacgtt gcgcggtttg catctggccc atgtcctgac cgtgccgttc gaaaatctgg
     4681 acatccacct cggtcgcccg atttcgttgg atccctccga tctgtttcgg aagatcgtga
     4741 tcagtcggcg gggcgggtat tgcttcgagt tgaacggatt gtttgcgctc ctgttggagg
     4801 agcttggatt tgtcgtcacg cgactggccg cccgggtgtt atatgggacg gaagacgtta
     4861 ggccgcggag tcaccaggtg ctgctcgttc atctggacgg agagcgctgg cttgtggatg
     4921 tcggattcgg agggcaggga ttgcgcgagc cgttcctctt agtggctggc ctcgaacatc
     4981 ggcaagggcc ggatcaattt aggctcgcga ccgacgagcg gggagagcat gtgctgcaat
     5041 gtcacatcga gaaagcctgg acgaatctct attccttcac gctcgatccc tggcttccgg
     5101 tggactacca gtttgccaac tattaccact cgcatgcgcc tgaatcgccc ttcgttcagc
     5161 gctggatgtg cacaatgccc accctgaatg ggcggaagat attcaccgag aaactgctca
     5221 aagttcgggg gcctgaaggt gtgcataaac ttcatgtaac gagtgaggtg gagtgcaagc
     5281 agctgctcca ggagcacttc ggattgatta ttaacgatca cttgagattt ttggtttaag
     5341 gggggcgtct agcgggggcg taaagcaaag gggggaggat ggcagcacaa aaaggtcgac
     5401 gtcccaagac ctatagccag gccgaccggc ttgctcgcat gttgcgcgca ctggcgagcc
     5461 ggtcgatgac agtcaaggat ctggccgaag ccttcgaggt gacgaagcgg caggtctatc
     5521 gcgatcttga tcgaatccac gaagaaggcc atcctctgga gcaatcggat ggggatggag
     5581 aaaagacctg gcacctgccg ttgggctata aggggctccc gccgattacg gtgtctccct
     5641 atgaactcat ggcgctacat tttgccaagc gtcatctcga ctatttgacg ggcaccccgt
     5701 tggtcgatga tctcgacagt ctcatcaaga aagtcgaggg cgggttgtct cacagggtgt
     5761 tcaatcacct ggcgcgcatc aatcaggtgt ttctgcctcg ctccgggccg gtgcgggact
     5821 atgcgggacg gagggttgtt ctcaagcaac tccagaaggc gttgctcctg cagaacatcg
     5881 tcgtcctccg gcatcgcagg ccggactacg acgagccggc ggaacatcgt gtcgatccct
     5941 atgtgctgct gctgtaccag tttggattgt atctggtcgg ctattcgtac cgggccgagg
     6001 cgctgcgcat gtttgcggtg gaacggatag tctcggttga agttcttgag gagcggttca
     6061 gtctgcccga agaagtacag gtggggaaga tctatgacaa cctgtttggc ttggtcgatg
     6121 aacctactca aaccgtccgc atccagttcg acccggatgt cgcctatctg gtgaaggagc
     6181 gccgttggca cccgagccag cagaacgaga ttcaaaaaga cggctccgtg atcgccacct
     6241 ttaccgcggg cggaatggat gaacttgcct cgtgggttct ctcgtggggg ccggcggcga
     6301 gagtgctcga acctcaagcg ctggttgaag ctgtgaagac tcacctggct cacgccttag
     6361 agcgctatcg ctaagaccat ctaagaaaag aaggcggacg gggcgtggtc cgaccgtcct
     6421 tcaagacacg gccgtctcac cgtctcgtcg gcgtgcgcaa acgtgacgct ctttcttcat
     6481 cgcgtcgcgc acctcgcaga acgcacacga tgaaactgtg ttcgtccgat gcgcgcagtg
     6541 gaaggacgcc cggaccacgc ccttgcctag aaagtataag caaagagacc gtgacattct
     6601 ctgtcacgct tgtatggtag cgtcctctcg tcaattttct gagaggactg tcatgctgac
     6661 gagacgaaat ctcttcaagt tcgccgccac acttgggctg ggtacgttcg cgccatggct
     6721 tgaccggtcc acccgtggtg cagccatgga gttgcgaccg ggtcgaaatc cgccttcgga
     6781 tagttttttc tcacagccag aaagatcgct ccaggagatg gaatcgcttg agaacctcaa
     6841 ggatgacttg gactgtgtcg aggtcgtcgc atacgtggct tcggcgcgaa gtatgacctg
     6901 ttcggccgag gtcaatctga tccgggggag ttcccattgt ccgttttgcg gtgaagcggg
     6961 ttttcgcgtg ggaacggaat ccttcacgtg caagtggtgc gaggcggagg ggtcggcaat
     7021 cgagttttat gccagggctg aaggactacc gcatgaggta gcggcggtgc gcctcgaagc
     7081 actgttgaat gacgggttgc tcaagggccg tcgccctgag aatgcacatg cgtacagaat
     7141 gttggaggaa gcggcgcgct tctaccatgc cttgctgtgc gaaagaccgg aaggggcagt
     7201 ggctcgggag tgcctggtcg aacaagggat cgcgccgtcc acgatcgaac ggtttcggct
     7261 tgggtatgcc ccgcacgatc cggatgatct cttaagccgc cacttggtgg cggaagggta
     7321 tgtgattccc aagctcctgc aaacggttcg ggtgaacgaa gcgggtgagg aaaaactgat
     7381 cgatcgatat ggcggcggcc acctgctcat tccaatttgc gatcaggcag ggcgtagctg
     7441 gggattcatg aaacgcaggc tcacctcgcg gcaggactct ctgcaggcag gatggacgca
     7501 ggacaccttg gccgtttcgg agcgacggct gcgcagactc atctttcccc atcccgcctg
     7561 gccgcaggaa ttcaaccggc accagagagt gttgattacg gagacaccct gggaggtcgt
     7621 cgccttgcac aatgccggta tcgagaatgc cgtctatctg tttgactgcg cgaccgatcc
     7681 cgttcgtctt cggacgctgc tgtctctcgg ccaagacttg atttgttgcg tcgaccccaa
     7741 ggggcgaagg tcggtgatgg ccttgcgcct tattgaagtg ctcgaacgtg aagggcggcc
     7801 acttcgcgtc atgtccgtgc cgtccaaggg tggcctcatg gaactgctgc aggcgagcgg
     7861 ccctcaggcg gtacgcaagg cgatgagtca ggctatcccg tttcatcagt ggctggaggc
     7921 gtgaggtcta ggagggaatg ttgtcatgga atggctgttg ccgctcattt tacagatcgt
     7981 gattcctgcc gattcactca tgcaggtgac tctgaacaaa tccctggcgc aggtcattca
     8041 ggagcagatc agttgccgca aggagttcga cgaggcgagg ccggagttcg agcgggtaaa
     8101 tagtattgat gacgcgattg ctcggctaaa tgccatcgac agattacgga ggtctgtgct
     8161 attgcggtgc ggtgtgaggc catgacgttt gatagatatg aattgagtaa tgctttagag
     8221 atgaatcgca tgggggagtg cctaaatgct tcaagcaatt ttgcatggaa aaattggaat
     8281 gttgtttgaa aagggggcgg agggagaaag ttttaaggag cggtttcgaa cccttgaaga
     8341 tttcctgacg gcggctgtat ttgagcgctt acgatatctt ccggctaatc ttttctggaa
     8401 ggttttgtcc gaagccgcaa ttccagtagg aagtttgccg atgcatgtag gaaacctcag
     8461 agagtctgag ttttggccta cttgggacat ctcccaggca acaagcgata gaatgagatg
     8521 catacccgat gtttttttat ctttcgaaaa aatagatatt gcggtggaag caaagctcaa
     8581 tgatggccag cgttctcaag atgcccaaca gctggcgctg gagtggcttg gatataacga
     8641 agagattgaa ggaaggcaaa tgtggttgat gatggtgagt gggttagagg aagtcaccga
     8701 tgattgcgtg ataggttttc gtaggcaaat tgaggaggcg ctgcgcaacc ttggcagaga
     8761 tggagcgcga gtgcagttag ttggaacttc atggcagttg attgccaaag cattgaagag
     8821 ggttgtatct gcgcacccaa ttgatgcgaa cattttgacc gtaatcaacg acatattgga
     8881 ggcgttccaa ttgtacaatg tccagtcgcg acaacctacc tggttggaga gtttgcgccg
     8941 tgaagcaagg cctttcgcct cgattcgcga tgaaagtttg gaacagtttg caggaaggat
     9001 ttctaatgga ttctcatagt gatgaactca agctggcttt ggttgatgtc agaaaagctt
     9061 ttcgtttgtt gtttttgtat caaaggcgaa ttttagattt ctccaagaca ttcgctaatg
     9121 aaatgggcct tcgaccgtat tccgcggtct atggattcaa caggccgact acagtgaatc
     9181 catttgataa gtgggtctgg gacatgcttc caatgtaccg tgtcagcttc ttgtttcttt
     9241 ctctctctac tgatcaaaat cagacgaaga aaggggaatg gttgttggcc attcacctcg
     9301 tttcggatac aggttacccc gacggcatta gttggaaaaa gggtgagcca aatcccttag
     9361 atttcccggt tgcggaagac tctttctcgc gtcttgggat ctatgcgttc aagagtgata
     9421 tagatgagag gcggaactgg caagaaagtg tttggcgtcc agcgatcaac tatctaccta
     9481 agtatccatc ggactggaat gtcgttacga tcgaaaaaat tggtgtatca atctgtggtg
     9541 cccaattcga tctttcggat ctgtcaaata aggaggcgat gcttcgcact gcgagtcaat
     9601 ttaaggaagt tcttgcgcgg gatctgcagt taagcttccc gtagtgtttc tcaggtaacc
     9661 tatccgttcg tgaccggctt cgtcatgttc ttagaacgcc tttttcgatc tcgcaaaagt
     9721 ccgctgaact acgattaagc aaagccatgt gcgcgagacg catagtgttc aaatatagat
     9781 ggtgattgtg gtttgtccct gtggcccgct actgggaaaa agggactgtg cgattgtcgc
     9841 tcattaagaa atttcgattt aatgccgcca tctacatgaa cgaactgacg gtaaatgatt
     9901 tcctgtcctt ctgaatattc ccctcgatgt tccttgagac agccttgccc tcggttttct
     9961 gtggcctcga ccaaatgcta tgcgcgatgg cctgtgtcgc caccaagagc aattacgatc
    10021 cgagatagtc ttgggtgctg tgactttgtg agtcacgcct ctgtggtagg ctctcctcgg
    10081 tcattttgga tctctatctg caacaacacg atggacacgg tcggcgatgc ctgtggtcag
    10141 gagtctcgcg ttccgccacg atcgactcat acgagcgcac aaccggtgac aggaggtctt
    10201 tcgatgagca ggtatatcta taccaactgg cgaggaaagc agtttgtgat cgatgaggtg
    10261 ttcgccgacg agatgaagca atctctccta gcggacggat gtgatgtaga gtttgtcgcc
    10321 accgtgacac cggagagcat tatgaaggat cttccactgc acgagaaaat tgagaggctg
    10381 cagcaattga agcacatgtg ggaaggccca gtgaaccctc gaggacgctg acgtctcctc
    10441 tttcattgac aagcttatcc agacctctcc ttgcctctcc ttgtgacctt gtctgtcaca
    10501 ctttcatcgt acgctgtcct cgttcgttct ggccgttcgt tcacttcaac cgaaggaggg
    10561 gaagccatgt tcacgaggcg cgcgttattc aaaatgttgg cggcgatcgg gggcggctca
    10621 ctattgccga ttgatgggcc agccggcctt tatcgaaggt tccgcttcaa ccaagccttc
    10681 ccggcgttcc cgcccgcgcc gggtaaaact gcgccgagtc cgtttggctc catcccggac
    10741 ctctcaatca tcgaacagtg ggaaaatctg ctggacgatc tggatggcgt cgaattgatc
    10801 ggacgctaca gtcggctcac gccggttggt gatggatcgc gcgactgtcg ggggctttgc
    10861 cagttttgtc ggcagggcac tgattcgttc agggtcagtt gccgtgacgg ctcctatttc
    10921 tgcaccgact gtcttgtata cggccacgcg ttggatttct actgcgagat ggaacggttg
    10981 actcccgcag aggcgattcc tcggctgacc ggattgctgg cctccggtgc tttgacgggg
    11041 aagcggctcc ggctggaacg aatgtttagg gtgcttgacg aagtgcgccg ggtggcctgc
    11101 gaagcgctgc agcatggttc cgcgggatgc gccgtccgtg agtggatcga acgggaaggt
    11161 gtgagtcagg agacaggcgg gcagttttcg cttggcctcc tgtcgaggga ggtccgtcaa
    11221 ggtctcgtgc tgcacctacg cgcccagggg ttcgaccagg ccgagttgga ggaaatcggt
    11281 atcaccgggt ggcttgctcg ccatgctgat aaatcagaaa tgagtatgct gatcccggtg
    11341 ggggatgtgg atgggagctg ccgggggttc tatgaacagg gcatgtacga ggatgctgac
    11401 gttatgtgga cctcctattc tcttccctat gggttcacac tgctttcgcc gcaccgcgcc
    11461 gatcggttgg tcttagccgc ggaggccggg cgaacgtcat ctgcatcggt ggtcttggct
    11521 gaacggcctt gggatgtcgt tctgctggct gaggctggaa tcaaagacgc ggtgtatgtg
    11581 gcagcacttg atccagcaga ataccgccaa cggctgaacc ttcagttgca gcgagtgcag
    11641 cgcgcgatct ggcccatccg gcaagacgac agcacagtgg agtttttgcg ctgtttgact
    11701 gaggggtttg gcaggtccgt caggcagcta cgtttcgcga tcttgccacc cggtctgagg
    11761 ctgcctgagg tgttccgacg ggaggggatc gagtccgtgc acgcgcggct ggccggggcg
    11821 aaaccattga gggaattgct ggggttctga ttgtcggaca acaggggttg ttccgaagat
    11881 tctctttcgc ctgtctcgaa ctacggaggg ttaagagggc cgccgtagtc gcacctcgtc
    11941 tcgtacacac cctccgacct ggtctgatag gtcgagcaac gccggccgtt ctcatccgag
    12001 gaatgtccga tcggagtcct catcggctga aacaggatcg aacatccact tactgagccg
    12061 agcagcgcga gcaacatgac ggaacggatt atcacggtgc gcctgcgtgc atgtgccggt
    12121 ccgccctgtt ttgatttcat tctgccatta ttcaagaatt cgccgcatcg aggcaagttt
    12181 gcagaatatt gtgcacgagg cgcggatgtg cgtcggactc catcgatttc tgcggcgcag
    12241 gaaaatgccg ggcggatcgg cgccaaggtt cgaggctgaa ggtcatgacg ccgctgtggc
    12301 gggaagcatc gaacggatgc gcgactcaac cagggtgata tacggcggac cgcggcgaag
    12361 aaatgttgcc aagaatgatt ttctcgcttc tttccaaaag accaccgatg ggtgaagagc
    12421 accagcctcg agcataaaag tcatcacgtg gacgaatcat ttggtgcaga aagcggttag
    12481 agacgccgtg gtgaagtcgg gcttaccaaa cgatccacat gccacacctc ccgcactcct
    12541 ttgtcacgca ccggctcgaa agcggctccg acatacgcac aattcaggag ttgttgggcc
    12601 atcgcgacgt caaaacgacg atgatctaca cccatgtgct gcatcgaggg ccggcgggtg
    12661 tccgcagtcc gctggacggg atgtgaaccc attcacgtga cgggtgttat gccgatccgt
    12721 ataagaaccc aggaagcagg ggcatacggt tgcaactcgt ggaaacaacg gcataaatcg
    12781 cgtatagtgt gcgaacgttc gccgcgtgtt atgcggcttg aaactgccgg tgccgcgctt
    12841 atgcggatcg gcctaaacat agttaggtgg acaagaccaa gatgacagac tttgctggaa
    12901 gctgggtcgg ttcgatcaac ggcacgaata tcgccacctt tactgtgacc cttgagcaaa
    12961 cggagacgaa ggtacttggt gacctgactc taactgacgc tgccttggga atcgcaacgt
    13021 ttaaggtcga cggcacagca ggcgacggag tcgctacgtt ccggctcatt ccaggaaccg
    13081 ccgcctcggg agtaggcctc agcaacggag tcgcacgggt ggctctccag ccagatggat
    13141 ccctcgctgg catctgggag atggaaagtc agactttcgg tgctgttact gccaaccgag
    13201 agatcaagac cgaagaactc gaaccatcac ccggtggacc tttgctgtct ggacgacctg
    13261 agttggatcg actcgcagtt ggccaaatgc cgtatcgctg gatgccaatc atgggcgagt
    13321 tcgaattggc tcctagatcg ctcgtgttca agggacgccg aatcccaagc gtcgcgggac
    13381 cagctcaagc tgaggggccg cagttccttg ccgccgtggg catcgctctg tgcgacagga
    13441 agctggtgaa tgggacgcta accgcagccg ttaggttcgt cgagatcagc ccgaattccg
    13501 tttgcgaact tattgtcgcc cacgacctga ggactaaggg ccagatcacc gctggccttg
    13561 gcggcagcgg tgccatgttc agtatccgcg agtggttgcc agcacaaacc cctcagcaaa
    13621 cgggtggttc gtgggtcaac tacgagctcg ccggtgatcg agcgaatctg cgcgctggaa
    13681 taacgtatca gctggtcgtg cgacttcatg gatcgaactt agtcttggaa gttgacggag
    13741 tggaagtcgc cgccactggg ctacaagcag ctcccaatca gcctcgccag atcggtgtgt
    13801 ttgccgtaag cgaaggccag gtgcgggttg cggatttctc ggccaatatc gagcggccaa
    13861 ctgcgtttat cgtcatgcag ttttcgccgc cctacaacga ggtctattct gacgtcatta
    13921 agcagctctg cgaggaatgc aggatcgatg cggttcgcgc tgatgaaatc tacgggcccg
    13981 gtatcatcat caaggacgta atcgatcgca ttgcgaagtc gcaggttgtc atcgcagaca
    14041 tatcaccgac gaatcccaat gtatattttg aagtcggtta cgctttggcg ctggggaagc
    14101 caatcattct ccttgcccag cgccgagggc cagagtcgcc gttgcccttt gacttgtcgg
    14161 cctttcgagt actgttttac gacgacagta tcggtggcaa gctcaagttg gaatccggct
    14221 tgagaaaaca tctgaaagag atactcggca atgggtagcg aggccgccta acaccgcaat
    14281 agagccggct gtgacgcgcc gcggctcaac gcgaacgcag gacggcatag ccacggaaca
    14341 gactaaccga agaaatgctg ccccgaaatg cttttctcgc atgcctggca tcctgcttgg
    14401 tttgatgctc aaacaggttc tgccgatttc cgctatactc ttcggtgatc cggcgcgggc
    14461 atcgttcgat gctcgcggct ttgttgaaga tcccatcgtc agccatagat cctgcaatcc
    14521 gaccttttct ctgcactagg cagtgtcacg atttgcctcc ttacgaccga tcgtcttgat
    14581 gctccccttt tccgcctttc cgttgagccc tctcctgaaa atggcctcaa gcagccctcg
    14641 caaattatga tagataaaat cgtgaccgtt cctcacgacg caattggtac cagcatcggc
    14701 tcactcgatc atgagatgat ggttcgcgtt gatcgctccc tcgccctgtt tttcgggatt
    14761 gtctgacctt catcttctaa ctgggttgtt gccggtcctg attgcctcgt tgcttggtgt
    14821 gattgtctgg caggtcgatt cggtcgcttg cgaatccctc acgttatgtg aggggcagca
    14881 actgccttgc aaatcaaaca ggctgactga cggaatcacg ccacgaggtt tggctgctca
    14941 ccatggcatt cagaatcgtg agcagtttcc gcatgcacgt cgtcagcgcc agcttcttcg
    15001 ccttccccgc gtgaagaaat ggtgacagga atgatgttct cacacaagcg gtgagagatc
    15061 atacggggca ggcgatcact ctgtccaaag cctatcccgg tggtggttct gctagttccg
    15121 cccgcccctt tctagccgga ctgattgcac cgaccagtcg agccctccta tcggcacatt
    15181 gcggccatcg acggcttcga tgaaaatcgt gatggccggt tgcgtaccca ggcggtcggc
    15241 caacgaggcg aggtggcgcg tcacatcgat cagcagggtc ggcaggcgtc gcggagccga
    15301 tcccaccgac cggtccccca cgatgcccgc cgagcccaac gtgacaggct ccgctccttc
    15361 gatgatcgca gccgctcgca gcttgaacgg ttgtccccct gcgaccgtca tgccctccag
    15421 caccaatctg acgtgggagg cctgttgcca cccggtcggt aagtcgacgg agaaccggtg
    15481 cggtccttgc tccgcctgaa ccgtcagtgc ggccaggatc atgacgctcg cgagccagga
    15541 gataagtccc atggttaccg ttcctcgcgt tccacctctg cgacggacag caagacgtcc
    15601 ttcgcccgta ccgggatgac ttttcgggcc tcaggtttaa ccccgcgctc gaccagggcc
    15661 aggcggaccg gaccgagggt accgctcagc gcttcgagtt tggcggtgga gagtctaacg
    15721 gcgatgtttc gtgcgttctt cgacacatgc cgacgctcgc gatcgttcaa caccaccggc
    15781 acggtaccga gatagccctt gctgccgggt ccttcatcgg ctttcgccgc ttcgggagtc
    15841 gcatagagtc gatacacagc gctcttatcc gtgggaacgg cgagctcgtt caggtgcagt
    15901 ctcgcgcggc ctccctgttc gacgactttc atcatcttag cacgcgtctg tccggcaaac
    15961 ttcagggtct ggctgatccg ccagtcggtc ttgatgggga accaatcgag gaggcagggg
    16021 agctgctcca agaacttcga cggcccatag gtataacgca attgggtgtc gtggttcagg
    16081 acctgtgacg ccttgatcga ccgccacact ttattttcat cgtagaaatt ccacgtcagg
    16141 ttcaagaagg ccgttgccgt cgggttcgtg tggatggacg aggccttgtt ccagtccgac
    16201 cacatcttgt ccacgttggc atgatgcgcg tagaagacgg gatctttccc ggctgaatcg
    16261 aagaagccca tgtcgccgcc gacgtcgacg tggaccgcgc cgtgtggtgt tccctccggg
    16321 atgccgctgc cgctcgtggt gccaccgaac tccgtgaagg tgtccgccgt gagggcggcc
    16381 tccatcacgt cttccccgac atcttcttcc gggatttcat ccgtcgggtc catgctgcgg
    16441 gtcccgttcc acagtgggtt gctgttgtcg ttcggatcgc tgtaggcgcc gggcagcttg
    16501 cggtggctcg gtgtgtccca gtcccagtag ggaaggcgga aattcatgtc gccgatcagc
    16561 ttcccgagga tccgttcgtg aaagtagaga taggcgcggt gccaggcgaa gaattgccag
    16621 gtgaaatgga cctgagtgcc ggcaccgcag taccagcaat ggacgttggc ctgccgcagg
    16681 aatccgcgcg ggtcattcgg ggcgctggta tcgagcgcgc gcatggcctt ataggcatcc
    16741 ttgagctgtg tgatctccgc cgcactcagg gtgctagcgg ggcggcgcgg gaggatcatt
    16801 cgacagtcct tgcgccatgg tttcggtgtc ccgggattgc ctggaggttc gcacacggcc
    16861 agcaccgatt ccggcaacac caggccgccg aacaggcccg cgcctagcgc cccgacgccc
    16921 aatcccatcc gctgaagaaa tgacctccgc gacggcatgg tcgcccacat gcggtcgtcc
    16981 agattcgaca tgacctacct ccttgtatgc ggcttcggct aggaaagctc cgcgccgtcc
    17041 cgagcgcgat gccatggttg gatggggcaa cgagtgatca atatccatgc catgagcgga
    17101 tgcggccccc ggttgcgaga gatgcgcgga ttctcggcct ggcgtcgaag gattccctgt
    17161 ctatcccgta ggtgttcgta tccaatggga tacggtgccg cccgaataga tacagctgcg
    17221 gtggaatttt tcgcccgatg cgatttttgc aaactctcat tcttctagcg atctcggtaa
    17281 aggaaaagca gcgctgtttt ctctctccta gctgtggcat ccggggttaa tggatctggt
    17341 ccatccagaa attatcgttg cgacctcagc tggcggacct accgtctgcg ctacaacttt
    17401 ttggcaatgg actggaaggt tctgaaactt caggtgagcc aagtccgact tatggcagat
    17461 tcgacatcga aggaaagagg ggtacgctga atctccattc tggatcgccg gatgtgtcga
    17521 ttaccgggat tccacatcta acaggctgcg gaaaaactcg atttttgcgc aatacaatgc
    17581 gatcggttca cgcggcgtgt tggtatcaga agcgcctgca ggatgcgcaa aaaggccgtc
    17641 cagcaaggcc gcagcgagcg aagtggcgaa tcgtactctt gccgtacggt gagccgctga
    17701 gcgatgcgag aacgccgctg gcggactttt tccgcatcct gctaagtagg tggaatccgc
    17761 tgacaaagga atcgtgagct gagattgggg agttaaattg gcgtggttgg ctatgacagc
    17821 ttgttcggta tcgtcccccc tctatgcaca agctttgacg atggttgggg agaaccggcg
    17881 aaagtcatcg agatgtttcg tgatgatcgc ctggacaata tcagggttca ggcgagtgta
    17941 ttcatgcacg gccacgttgc ggaagccgac catccgctgg agtcggccgg ccaactcggc
    18001 agagagaagt tgagccttct ccaacagtgc gaacgcgtcc ctgctttctt gtggaaggcc
    18061 taagccgttt cgtctcacga catacatggc cagatcgatc gcggtttcgc atgcgcgttg
    18121 aagattgagg acgatggcat cctgcttcgt ttgattctcg tacagatgtt cccgcttgcc
    18181 gccatagtcc tcgcagatgc gggcgagacg acgttcgata cttgccgcct tgttcagcac
    18241 cacgtcgtca gccatagatc agcccgcgct ccttgatgtt cttgagtagc tctcgccgtt
    18301 cttcgttgag cctggcgtag tcggaatagg catacatctc gaattcggcg cgggcctggt
    18361 cgtcgtggct tttgaggcat cggccggtgc tgataatctg ggcgcgcatg accgtcgtcg
    18421 tgctcctcag atccaggagg tccacgttac ggcccacttg tatggcgagt tcctgcgcca
    18481 agtgaaagag ccgttcaacg cctggaaaaa tgtcgaagta gaccgctgcg ctcaaggatg
    18541 tgttcccgcg ccagcagctt cacggccatg gggaaacgag gatctgaagc agcatctgtc
    18601 ttgagaggag ccgcctcgcg atagatatcc ttgcgatgtt caattgccag gatatccaac
    18661 tactcgctgc cggtcttccg gtaaaggatt cggtagggct aaacccggta ggaccagacc
    18721 ttatctttct gaaatttccc cttgagcgtt ttgccttcga gggatggtct gcgaggtggc
    18781 ggatggcgtc tttgattgtg tcgcgggttc cggcgtcgaa ggaggcgaca tccttaattg
    18841 cctgtggggt gagccccgcc tgtctcactg ctgttcgtcg aacacgtcct tgaacgaccg
    18901 aagtttccct gccttcgctt ctttcagccc ttgctcaaga gctttgaccg cttggggatt
    18961 tgagagcagc tccaatgttt caacccaggt ttcaaattct tccgccccca tgaccaccgc
    19021 tttaggagtt ccattttttg tcacgattac ccgctcggac aaacgatcgg cattgtccac
    19081 caaggaggag aatctggacc gggcctgctt gagcgacatg gtttttgtca tcgtcatgcc
    19141 tcgttgatgt ggcgtgtaca caattgtgtc tcgtccgaca aggtatcagc cctaccgcgc
    19201 cagtagcttt accagcccca cgcagcacat gtagagggcc attgagccgg ccatggagag
    19261 ccagaagccg acgcgttcga ctccgtaaat catgcagccg acgtagacga gccagggcgg
    19321 cacaaaccag agcaggttct tggcgtaggt cacgaggtgt tcgctgccgc cgttgagata
    19381 gatgaggatg aacgtcgcgc cggtcatcgc cggaaaggta ctggcgaagg cggcgagaaa
    19441 cgaccggcct tgtgatccca gataggttga gatgctgaca atggtgccgc ccaacaagaa
    19501 gtacaatccg tacttcgccc actcgcccat ccgcgacctc cttcagctgc ctcgaaatac
    19561 ctgtcgtgct tccgcctcga tccggtggcc gagtcccgtg tagcgcgggg agaaatggaa
    19621 cacgaccaac tcgcgcacgc cggccttctt cgccatgact ccggcctggc gcgcggtgag
    19681 gtgatagcga tccctcgcct tgtccgcatc ctgatccagg tagggggcct cacagtaaaa
    19741 gatgtcggcg ccttgcgcaa gctccacgat cttggcttca ttttcttcgt cgtatcgcgc
    19801 atccaccacg taggcgagtt tctgtccgcg gctgatggtg atgaatcgct cacggacctc
    19861 gcccaggaca aactcgcgtt cctccttgtg atgttcatgg tagagcgtgg cctgaaatcg
    19921 aaagtcgtcc ggcttgccct gccagaggta ctgcttcact tccttcagcc aatagccgat
    19981 cggcaacccg gcttcgtgca gccgctcctt gttgacgttc acatgaaact gttcctgcag
    20041 cgagtaggcc agggaaggaa tgtggtgatt gagtgcgact gcttcgacgg tgaacatggg
    20101 gtctgtgagt acggaaaacc gacggccggc tcgccgatcg atgggggctc cctccggagc
    20161 gtcgtgacgt tgaaatccgt cggtcgccag gaaagtggcg cgccggattg tctgctcgtg
    20221 gaattcctgc acggtgatag tgagcggata cccatccacg aggttccagg tgtagccatg
    20281 aagttttcct tgaacattgg cgatgagtcc gggcggcccg tagaggcgga gtgttttccc
    20341 gcgcccaagg gcgacgcgga gcagcgcatc gaagccgaca aagtggtcca tatgtgtgtg
    20401 cgagatgaag atgtcgccgg cgcgcaggag gcgggtcggg cccagggcat cgttgtggcc
    20461 cagatcgaag aggagagcgc gtttggacca gcgaacctcc acgaacaggc ctggatcgcc
    20521 gaagacatca ttgacgaggt agcctgagaa ggacgggttc atggtttgat ggagcacagc
    20581 atcactttat caggagcggt taatccgtca tgatcgaaac ttatacttta agttcttccg
    20641 gctgccggag caggggcata tcaggtcagt atcactcgca tgacgccgta taagacgatg
    20701 acttcgacgg cgtgggccac gatggtggca tagaggtttc tgagcatgac ccatagggta
    20761 ccccacatca agccgtccca gacggcaatc caatgcaggt gctgaaaggt tgccaccaga
    20821 aagggatcga acgcaaacag cagggcgctc gccggcacgg caatcggagc cgggagcccc
    20881 agggcgagga gccgccccag gagaaagccg cgaaagttca actcgaccat ggtggcgatg
    20941 agcaggatga accagggcac catgatcagc aggggaattt tggcgtgcgg ggtatcggcg
    21001 agaaatctgt agtctccccc aagaatggga atgaggtaga ggatgaccag cacattgatc
    21061 ggtccgagtg tcaggccggt gagcaggccc caacggaggc cctgcgggac aagcgggcga
    21121 gcgagcccga ttcgacgggc gacggatcca ttgatcgtgc tccaggcaat gaaggcggca
    21181 taggcacagg cttgcgggaa aaactggaag aggctgtgtt gttggagtgt ggtcggtgag
    21241 ctgtaaaaca ggattgtggc agtcaggggg agcagcacga gggctgctcc ccgttcgctg
    21301 gcctgagtcg catgagtcga ggacgcgtcg tcaggcttga tcatggacag gttagtcgag
    21361 ccgcaaactg tatcgctcaa gggtggtggc tttgttacgg gccagattcg acagcgactt
    21421 gttgtaatcc aacaccgctc gaagctcgtt gccttgggcg gtggccaggt cgcgctgaaa
    21481 gtcgagcacg aagcgcgtcg tactgagacc gaccttcagc cgttcctgtt cggcctgtag
    21541 ttgtttctcg gccatgatac gggcggagcg ggtggtttcg atgcgcttga aatcggtgtg
    21601 gacgcgacgg accgcttccc ggacaccgac gatcacctgc tgacggacgc tctggagcga
    21661 cgactgggcg ttacgggatt ccaattgccg tttgttgtag gtgctgtacg ccgaccggtt
    21721 cccgatgggg taactgagga ccaggccggc gccgtagttg tagaagtcac cgcctaaatt
    21781 ccgtctcgtg gcatcgccgt agtctgcgcc gagccccgac agtcccatcg tgccctgtac
    21841 ggacagggtc gggaggagtt ggttcttggc gaacttcaca ttcaggtcgc tgctctccac
    21901 attcttccct gcttgtaaca cctccggccg gcgctccatg gcgatatcga tggcttcttg
    21961 caagctgatc gcctccagtg atgtgacagg tggatcggtg gggatcaggc gcaagtcctg
    22021 ccggagttcc tcctcgacgg ggttcaggag gcggcgcaac tggtcctcct gatcgcgaat
    22081 cgatttttcc gcgacgagga tctgctcgac tcgcgaggcg acggcggctt cggcctgcag
    22141 cacatcgacg atcgacatga ccccggcttt ggctttggcg cgattgctgg ccagcagttc
    22201 ttccgccgcc ttgagcgccg cttgcgcgac tttgagattt tcattcgcga acaccatctc
    22261 ccaaaatgtc tgttcaacgc tggcaatgac ggtgagcaca cggtcgagaa agacatgttg
    22321 ctcgaccgtg gcattgttct gcgcgatcga gatgaacgtc ttgttgatgt cggttccgaa
    22381 gttcttcagc aacggttggg tgaccgtcaa ggcgagtccg ccggtccagg ccgggttgaa
    22441 gaggaaggtg ttggggccgc tcacgtagct gcgttgggga ctgtagttca aatcgtagtt
    22501 ggcgccggtc ggaagattct gtgtgatgtc ggcggtgagc gagtgggagt tctggtcgaa
    22561 tttggtgatc tcctgcaggt ttgccccggt gaatcccagg atcggccggt tgagcggtga
    22621 gacctgacgg ttgtattgcc cgttcaaact gacggtcgga tcgaacttcg cctgctcgat
    22681 cacgatgtcg gccaggcggc tctctctcgt ttggcgaccg atactgatat cgagattgtt
    22741 ctgcaaggct ttcaggacgg cgtcggcgag cgacatggcc tcccggcgtt ccgtcgggac
    22801 cggcttgttc acttccaggc cgaaggaatc gggtgccgtc cagaggagga gcgccgctgc
    22861 gcccccgatg aaaaggcacc cccggaggat tgcattcgtc ataacaaact ccttgatgcg
    22921 aggtctgcgt tgggccatac tgaattcaaa atgatgctgg cgtggtacca taaacagaag
    22981 tcagtgtcca tgctgaggag cgtgtcggtg gctggtctga cgctgctgct gacttccccg
    23041 gcagcggcgc aatccgtctc gagtgtacga ggtctggggg gaggggtcgc gccgctgttt
    23101 ggtctggtgg ggccgggaaa cctctatatc gataaccagg ggacgcaagg gtacatatat
    23161 aacttcggga ataacttcga atcgtataac ttccgcaatc cggcgaccgg gcaggcctgg
    23221 agcggcgccc tgatgacgct cggtccgcaa ctctcggtgg ggctgattca gggtgcgaat
    23281 caagctggat cgcccgtggt ctttcctccc gtcccgcgtg aggcggggcc gctttctccc
    23341 gttcagtcgg gtctgctcga cgacctcatt ccctagtccg gactattcat aaatacctgc
    23401 ttcggcgtcc gatcctgtcg cccggcgagg ctgttctcgt tcggcctcct cgacggactg
    23461 cacgtacgcc tgcgtcggcc tgacgtgcga gccgcctcgg cgggcttccg tctcgaacgc
    23521 ctcgcgacgg cattcatgaa tagtccgggt taagcagtat gttggatcac ccgctcggcc
    23581 gcgttggcgg atcatcctct gcggcggatg gtctcgcctt tcgatactca atatttccct
    23641 gaacgaaggt tttcttcttt gtggcgcccc gcgtctgtcg taatggatgc gtgcgcaagg
    23701 cttcgtcgaa gtagcgggaa tcggacggag gacattttct gagcgcgaag caggaaatct
    23761 ctgtggcggt ttggtttgtc gacgcaagcc tgtcacagcc gattcgggcc gggagctgaa
    23821 ttatcaacag aatccacagc ccacaaatcc atcgatgtcc agatgaaatg ttgaggtggt
    23881 tatgctaagt tattgattaa taattgtttt gaatgcttgt ttgcgtgagc ctcactcctg
    23941 aggcacgtgg cggtacaacg cttgctttgc cctccggtca tcgcgagcgt tcactccatc
    24001 ggcatcaaga cgacaaaaac aatcagcccg tgtcaaagat gtcaattgaa tcggataggc
    24061 agaacagaaa gaatgcggca gatggtggtg ggcgtggtgt aatattcgac gccgtttccg
    24121 gcaccggccg ctgaccgttc aagactaaag gagattgtcc ttatgaggat tgcccaggta
    24181 tcgccgctgt gggagagcgt ccctccaaag ttgtacggag gcacggaacg catcgtctcc
    24241 tacctgacgg aagaactcgt ccgtcaaggc caccaggtga cgctgttcgc cagcggtgat
    24301 tcggtcacgc gggcaaagct ggaggcccct tgccagcagg ccctgcgttt gaacaccggg
    24361 atcttcaacc gcgaagcccc gctcattcag atgatggaac aggtcttcgc ttccgccgac
    24421 cagttcgatc tgattcactc acatctcgac tttctggcct tttctctctc tcgccgatgt
    24481 cgggtgcccg tcgtgacgac gctccatggc cggttggatt tgcccgaact cgtgccggtc
    24541 ttccgtgact ttgcggagct gccgctggtc tccatttctg attcgcagcg tcggccgttg
    24601 ccttggtgca attggcaaaa caccgtctat cacggcctgc cgcacgacct ctataaattc
    24661 catcctgaac cgggtaagta cttggcgttc ctggggcggg tctcgccgga aaaatgtccg
    24721 gatcaagcga tcgaactggc taaacgtgtc ggaataccta tgaagatggc ggcgaaggtc
    24781 gacccggcgg accgggccta tttcgagcgg gtcgtcgagc ctctcctcga ccatcccttg
    24841 atcgagtttg tgggagagat taccgatgcg gagaagagcg attttatcgg gaacgccatc
    24901 ggcctgatct gcccctacga ttggccggaa cccttcggcc tcgtgttgat cgaaagtctt
    24961 gcatgcggca caccggttct cgcctaccga cggggatcca tcccggaaat tatcggtcac
    25021 ggtgtcaccg gcttcatcag cgaaaacctc gacgaaatgg tgagtcaggt cgacaagctc
    25081 ggcacgatcg atcgcaatcg gtgccgacag gtgtttgacg agcgattcac tgcccagcgt
    25141 atgacgaccg actacgtaaa gatctaccaa cagctaatca ctgatgccgc tgcgctccct
    25201 ggcaagtccg ggcagcagca cgcttcgaac ctctagtcag cgcgacatta acctcgcgga
    25261 aggacaagca tggcagctga gtcgcaatct acccctgagt cgaacgtcga cgggtggcgg
    25321 ttattaggca cccgtgattt cggctgtctc tgggccggcc aggtcgtctc tcagatcggt
    25381 gacggtttga acaaggtggc gcttctctgg ttcgtgtatg aaatgaccgg gtcggcgctc
    25441 aagatgaccg ccatcgggtt gttacagacc attccccccc tgctgttcgg tccgctgatc
    25501 ggcgtctatc tggactacct ccccaagaag acggtcatga tcgtggtcga tttcttgcga
    25561 gcattgatgg tgctcctgat accgctcttc tataccttcg acatgctgac gcttgagcgg
    25621 ttgtatgtgt tggtcttcct catctccatc gtctcaacgg tgttcgggcc cgctctggcg
    25681 tctgcggtcc cgctgatcgt tcagcggtcg caactgacga cggcgaatgc gtttctccag
    25741 agcaccacca acatcggcgt cttgctggga ccggccatga gcggattggg catcgcgttg
    25801 atcggcgctc agaatgtgct ctatgtggat gctgcgacct ttctggtgtc cgcgctcttc
    25861 ctcctgccga ttcgtgtgcg ggacagccgg acggtgaagg ggctcgatgt gttggcgaca
    25921 cccgtcatgc aggatatgat ggtcggattc cgcttcgtgt tcctccagca tcgggtggta
    25981 ttcgcgttga tgattaccgc cgtgctctac aacctggcga tcagcgcctt cgtattcctt
    26041 cttccggtgg tggcgaagga gttgttgcaa gtcggcccca tggaactggg ctggttgtgg
    26101 tcggccttgg gtatcgggat gctggcagcc tccatttggc tggctcggac gccccagggt
    26161 accttccagg atcgcatcgg caagatcggt cgttctctgg cgatcggcgg tattgcggtc
    26221 tgtgcgttgg gattaattca gacaccggtg ctgttcagca cgttcttgct gatcgtcatc
    26281 atcggcggaa gtacgtctct cttctacccg gtcgtatggg cgatgctcca ggaagtcact
    26341 ccggaacatt tgctgggacg tgtgttcacc acgttcagcg tcggcgggat ggcctccgcc
    26401 atggtcggga tggctgggtt cggatgggcc gctgatacgc taggtccagc tgtaagcctc
    26461 atcgggatcg gactgctcct cttgctcact gcgatggtca cggtgcaggt aagccgccgt
    26521 ggccacgtgg tgagtccggc tgttgcgtaa tactctccct ttacatcccg tttttgtcag
    26581 cctgcaggtc atccaggcgg cgcttgcagg ttttttcttg cccttgcgcc ggctgtctag
    26641 cggggcattg tcggtgttgc tttctgctct tccgtcacgt atgtctttgc ttccggggat
    26701 gtttcgtttt ccgagacgat ggtgaagacg ggctctcggc gatcggcttc gcagtctcgg
    26761 tttcattcaa tacatgaggg gcacatgcct atgcgggtga ttcgaaccgt ctacgtgaca
    26821 ggtgtcctgg cgctgttagc ggcgtcggga gtgggaaatg tgaatgaaac tgctttggcg
    26881 gaatcgacgc ctgagggaaa gtctgaaacc ccgccggtgc aacgaccgaa ggatcccgat
    26941 acaaagccga aggagccgga gccgaaaacg aaggaggctg actcgaaacc caaggaatca
    27001 gatgccaagc ctcctgaaaa gcccaaagag gcggaggcga aacccaagga gccggagtcg
    27061 aaagtaaagg aaccggagac caaggccaag gatcaggagc cgaaagcgaa ggagccagcg
    27121 gcgaaggcca aggaatccga acacaaggtc aaagaggcag agccgaagtc caaggagccg
    27181 gagtctaaag caaaagagcc tgatgcggcg gctgaacatc cctgtccaac ggtgccccat
    27241 gcggcagagg tgaaaccggc ggcagagacg caggcaccgc cgggagagcc cggtgcagga
    27301 gagcgcccca aagcggcgga gccggaaggt aagaaaccga tacgttcgtc gatggtgacg
    27361 gccaagctgg cgctcatggc ggatccgcac ctgtttcctt atgacatcga ggtcgatgcc
    27421 aaggacaagg atctcgtcct gctcggaaag gtgtcgcagg aatcggataa gcgcgtggcg
    27481 acggacatcg tgcggtgtct cgacggcgtg cacgccgtcg agaatcgact caaagttgaa
    27541 caggacgcgg cccatgggct cttcgcggaa cgcgacaaaa tcatcacgca attggtgaaa
    27601 gaacggttcg agaagagcaa aaccttgcaa tcggtcaagt tcgatgtgaa aactgaggac
    27661 ggcatcgtca ccctgaacgg ggcgacgcgg ttccagatca tcgtactcga agcggcgcaa
    27721 gccgcccgac aagttccggg cgtgcgcgcc gtgaataccg acgccgtgcg tttggtggcc
    27781 ggagaatagg aacggttgtg acgacagggg tacaggaggt ccgacacgca gtctgctgac
    27841 accatggagc cacggcacgt gaacggagga ggaacgggac gccccaacgt gaagtggtcg
    27901 ccgcatctgc tgacgcgcga cttcacgctc gtctggtggg gccagatggt ctcgcaagtc
    27961 ggcgatggtg tgtcgaagct cgcgttgctc tggttcgtct actccatcac aggttctccg
    28021 ctcaagacca ccatgatcgg gctgttgcag accctgccgc ccattctgtt cggtcctttc
    28081 atcggcgtca tcgtcgatcg cgttcccaag aaactcttgc tgatcagcag cgacctgatt
    28141 cgcgcgctgg tgttaggcgt gctgccctgt ttgttgccgg tagactcgtt cagtatcgag
    28201 cggctgtatc tcatggtgtt cgtccatgcg gtcgcctccg cggtgtttgg tccggcattg
    28261 actgccgcca ttccgtcgct ggtctcccgc cacgaattca ccgccgccaa tgcgctgctg
    28321 cagacgacga ccagcatcgg gatcatcgtc gggccggcgc tgagtggcgt cgggatcgcg
    28381 accatgagtt ctcaggaagt gctctgtgtg aatgcggtca gctatgtcat ttccgccgcc
    28441 tgttttgcgt tcattcgctt tccacgggcc gagtctcagc cggtcggcgg cggctccctg
    28501 gcgggaacct tcagcgatgt gctcgatggt ttccactatg tgctccatcg ccaacgggtc
    28561 attttgatgc tcatcggagc ggcgtccatg tataccttcg ccacgagcgc cttcagcacg
    28621 ctctttccgg tgttcggcaa gaagttgctg gacctcggcc ctatcgaggt cgggtacctc
    28681 tggtcggcat tcggcatcgg gttgttactg gtgtcgctgg ggctcgtgtc cctgtcggcc
    28741 tggtcgttgc ctaagcggat ccagttgatg gcgctctcca gcttcatcag cggcctggcg
    28801 ctgctcggtt taattgtgac gtcgaatcgg ttgatcgcgg cggcactcat ggtcatcatc
    28861 gggatggggg ccgggacgct cacgccggtg gcctggggcg tgttgcagga gattgccccg
    28921 gcatcgctgc tggggcgggt gctggcgatt tataatcttg gcgcgatgac ctccgccatt
    28981 gccggcatga cgattttcgg ctgggtgacg caggatttcg gcgagcgtcc cagcgtgttc
    29041 gggatcggtg tggggttatt cctgtcggcg ctggtttcga tgcgggtggc gcgctgggtg
    29101 cagaccaatt gggcgggggc gactgttcct tctgaggagg agtcgcagtc tgtggtcatg
    29161 gtcacgcagc ccacgagtca ttaatagcat tcagcgggag gtcgagggga tcggccctct
    29221 ctggaaggga agccggacga ggcgcagctt gagtaggcga aaaaacggga cgagggcgtc
    29281 gtgatgtcgg tcgtgactcg tcgcgtcggt gattctggga gcagcggagg aagacgtgga
    29341 agaaattatc agtgtcaatg atcagttcta tatcctggcc agttcatcga tggcggacga
    29401 tcgcacgcgc gtgctcaagc atggggagac gttcggcgtc tacgatcgat atggggatat
    29461 tcagccggtg ggccggggaa cccagggtgt ctttcaccag ggaacgcggt ttttgtcgcg
    29521 ccaggaattg tttctgaata atgaccggcc gatgctgctc agctcaacgg tcaaggagga
    29581 caatgccttg ctggccgtgg acctcacgaa tccggacttg tatcgggacg gccggatcgc
    29641 aattccacgc gggagtgtgc acgtattccg gtcgcgcttc ctttggaacg gggtgagtta
    29701 cgaacgcttc cgactgtcca actacagtct ggcgcccgtg aagatgacgt tgtccatccg
    29761 gtttgaggcc gactttgcgg atatcttcga ggtgcgcgga aaaaagcgtg agcggaaggg
    29821 gcggatgctg ccaaatgtgc tgcggaagga cctgctggtg ttgaggtacg aaggcctcga
    29881 tgaggtgacc cgggaggcgc ggattcagtt ttctccggag ccgctggaga tcacggcctc
    29941 acaagccacc ttcgatattg aactcgaccc gaagggggaa acgacggtgg cggtgatggt
    30001 ggcttgcgat gtgggggaca gccatcggcc cctgttggca tacgatgcgg caatggccga
    30061 agcgggcctg gccttcggtg cggagcagac cgaggattgc acgattcaga catccaacgc
    30121 ccaattcaat gaatggtgga atcgctcggt gttggatgtc cgtatgatgg tcaccgacac
    30181 gaatgagggc ccctatccct atgccggcgt gccctggttc agcacaccct tcggccggga
    30241 cggggtcatc acggcgctcg agtgtctgtg gatcaggccg gaattggcgc gcggcgtcct
    30301 ggcctatctg gcatcgacgc aggccaaaga ggtgaacgct ggtcaggatg ccgagccggg
    30361 gaagatcctc cacgagactc gcaagggtga gatggcggcg ttgaacgaga ttcccttcgg
    30421 cctctactac ggcagtgtcg attccacgcc gctgtttgtg atgctggccg gcgcttatta
    30481 cgaacggacg gccgatctgg cgtttattca gtcgatatgg gccaacctgg aagcggcgct
    30541 gacctggatg gacaccttcg gcgatcccga ccgtgacggg tttgtagaat atgtacggaa
    30601 atcacccacc gggttggaca atcagggctg gaaagattcc catgattcga tttcgcacga
    30661 ggacggttcg ctggctgaag gaccgatcgc cttgtgtgaa gtacaagggt atgtgtacga
    30721 cgccaaagtg caggcgtcga agctggcaga tgcgctcggc tatatcgatc gtgccagtca
    30781 gcttcggcgt caggcgcggt cgctcaagga acgtttcgaa gaggcattct ggtgcgagga
    30841 atcgtcaacc tatgcgcttg ccctggatgg ccagaaacgg ccgtgccggg tgaaaacctc
    30901 caacgccggc cattgtctct atacggggat cgcaagcgat gagcatgcca ggcgtgtggc
    30961 ggaaacgctc atgtccgatg aactgttcag cggatggggt gtgcggaccc tcgccgattc
    31021 ggagcgccgg tacaatccga tgtcctacca caacggatcg atctggccgc acgacaacgc
    31081 catgattgcg gtcggtctgg cccggtacgg cctcaagtcc ggtgtggaaa aaattatgac
    31141 cggcatgttc gaggtcagtc tcgtgctcga ctttcatcgc ttgccggaac tcttctgcgg
    31201 attcgtacgc agaccgggcc agggcctgac gcggtatccg gtcgcctgta accctcaagc
    31261 ctgggcggct ggctcggcct tcatggcgct acaggcctgt cttggcctat cgattatcgc
    31321 gtcggagcag aaagtggtgt tcaattaccc catcctgccg gagttcatcg acaagatgca
    31381 gatcaagaac ctcaaggtgg ggaccgcatc ggtcgattta ctgcttcggc gccatgacct
    31441 cgatgtgggt atcacggtca ttcgtcgtgc cggaaaagtc gaagtggtgt cggtgaagta
    31501 acggcgcctg cgtgaaggct cggccgcggt gtgagcgtcg gctcgataac atccggttgg
    31561 gggcagaacg gccagtgagc cgctgaagtg ttagggcgcg tcggcgagtt gccaggctat
    31621 ggtgagtatg tagcgcgcgg cggctgagag aatatctgcg ttgacggtgt ccgccgtgtc
    31681 agtcggctga tggaagtgcg gatggattcc gccgctcacc acggttacgg tgggaactcc
    31741 cgcttcctta aacggcacat ggtctccccc cgggaaaaaa ccgaacagat cgatacggtc
    31801 cgcaagtccg gcccgttccc cggcctgctg cgcagcgggt ttttctaaac cggtcacccc
    31861 gatcgtaagc cgtccgtttc cgactgccgc atgatccaca ttgatcatgg ccgcggtagc
    31921 ggtcagcggc accattggtt gagtcacgta gagtttcgat cccagcaagc cctgttcttc
    31981 tccgctgaac gacaccacta tgatcgaacg tttcgggccg acgggcatgg aggtgagtac
    32041 ccgggcgacc tccaggatca cggcggtgcc tgatgcgttg tcgtcggctc cggcaaagag
    32101 cagcccgcct tgttttccaa aatgatcgcg gtgagctccg atcacgatgg cgtcgtgttg
    32161 atgtgcggca tcctgtcccg gaaggagtgc gacgacgttc tgaagcgtgc cgttttgttg
    32221 cgtactgcgc caccgcactt caatcgagac gtcggtggtc gtggactgcg gggccatggt
    32281 gtcgttgagt tgctgctgaa ggcgccgcaa ccggttgtcc tcgccgggct ggtgtgcgtt
    32341 gaggatagct gaggcgagag cggtactgat ccatgcgccg ggaatcgtcc ggtgagggtc
    32401 cgtgagtccg tagaaggcgc tcggccgacc cgtgacccca cggcgggttt cataggcatt
    32461 gaggatcggt cctgtcgcgg tgaggtagcc gatggcgcca tgtgcgtggg ccatgtgcac
    32521 tttatctgcg tgggagacct gcttggcata ccgttcaggt tttccccgga gaaacagcac
    32581 gactttgttc cggacgtcga tccccgcata gtcatcgagt ccgccggcct gatcggagat
    32641 tccgtatccc gcaaaaacaa ccgaggtctc cagctctgct gaaggggaat cgaggattgg
    32701 taggtagtcg gtgccgacca cggaaggttg actctcagcg atccctccaa tctggagcag
    32761 tgaatcatcg tcgatctgag ttatgcggac gggggcggac tgaatctggg tgtgcgcggg
    32821 gagtcgatcg ggcggggcgg gagactcgga gcccggtgag cgatgctgtt gtagctccag
    32881 gaatcgttgc cgcacgaact ccgcggaagc aaggtcgtca ggcgtcccgg tctggcgtcc
    32941 gttgaatgcc ggtccgctca acgcgcggat atccgccagc atgcgctcac tggaaatctg
    33001 actcaatgcc tgtgtcagcg ccgtggacgg ggcgcccgtc tctgcggcga tactctggcc
    33061 ggtggccagg gccatacata agatgaacag caggtagaac gggagtcgat caatgtatga
    33121 cgattcagtg cgcctgctgt ctcgccggca aggcgcaagt ggtatggagc gatcgcaggg
    33181 catcagatag acgtggcaga ggccttttgt gccgaccagc gttgatacca tcgccagagc
    33241 ggtggctgca gaaagcgggg gatcggatac cccaggccgc gaacatagcc gtcatcccgg
    33301 tccagcacgg cgaatgccag ccggtacttg tcacggaaga tcgcgcagcg gtgttgcacc
    33361 tcctcgtcgc tcagctggat gcgcgcccgg atcgcggttc cgatggcctc atcggatgcc
    33421 gcttcacgga ccagcggttc gatgtaggca ggcgtcagct ggaaccggcg catgaaggac
    33481 tggtccagtg cggaggggta ggcataggtt ccgagcgtgc cggcctgaaa ggcgcgcacc
    33541 ttgtcgatca accgtggcag ccacattatg cctgccaggg tgtcactcca tcgtcgcggc
    33601 ggctgctggg ataggtccat gcggtgcctc ataatcctgg gtaggagagg tttcttgcga
    33661 accgtaggca ctatagcata cccggtcgat cccattgcag agagccgagg gctcaatgta
    33721 ctatcggcca tcaggtgagc gtcggggccg ttccttcagg agctgatcct ccgtgagggc
    33781 aggtcgggat caccaagcga ggcaagaggt aggatctatg gcagagcagg gaaagggtct
    33841 gtacgaccat cagtacggac ggtttcgcga gaatcaaggt acgcatcaag gctatgaatt
    33901 tcagcacggg cctgcgggga gcgtgcctcc ccccgtggtg acgttcatgg acaagataaa
    33961 gtggtggacg ttgcgcggct ggcgacgaaa gaagatcctg gctgaacgcg atcgatttca
    34021 acaggacatc atccggatca agcagaccca gccgaaatcg taagatccgc tcgaaggagg
    34081 agtgctcccg tgcaatatgg acacgagctg gtaaggcaga tagagttcct acgtgacagt
    34141 cagtcgcttg ctatggcgca tctcaggaat atcaggagat acgccggtgt catcgtgttg
    34201 accctgatgt cggtgagcgg ggtggaatcg gtgctggcgg tgccgaagga aacgtctacg
    34261 gaaaaggcgg agcatttgcg gcaacaggcg aatccccagc tcttcaacaa ttggacgttc
    34321 gacaaggatc aggcgggagg ggttccaaag gggtttcttg ccgtggcttc gggcgatcaa
    34381 ccaggcgggg actggaaagt tgaagcgcaa tcggcggcac cttcctcgcc caatgtcgtg
    34441 ctgggggcat cttcctgtcc cgcctgtgtc gagttgttgg tcgcgcaaga atttcagtat
    34501 gagtaccccg agctggtcgt ccggattcgt cagggagccg atgccacgtc cgggcagatt
    34561 ggagtggtgt tcggcctgaa agatccgaag aattattatg cgaccgtggt ggatttgtcg
    34621 cagaagtcca tagaggtggt gcgggtgatc gaaggcaaag agtcgatttt aggccgggcg
    34681 gcgattaagt cgaagccggt tgaatggcat acgctccggg tccaacggaa caccatcatc
    34741 agtaaggatt ttgtggagac cttcttcgac gggagcctgg cgctttctgt tgaggatcaa
    34801 acgcttggcg tcggacaggt ggggattctg gtgcgtgggc aggcgactgc gcgattcgat
    34861 aatttcaacg cttcaccatt gtattccagc cgtcccttgt cggcgcccgc ggcctactag
    34921 gcgcgatgcc ggcgggcctg ctcgactcgg agaccatttc acgcttgcct ttcctatttc
    34981 ggtaggatac agtgaaggtg cttcattgcg aagagccttg gcggtgaagt agaaaaagaa
    35041 ttacagggct cgatttcgat gttgttttgg gttcttcccg ggaatttcgg aagccagagc
    35101 taagattgcc gctgtgtttg catcttcgcc ccattccttc ctcgtttttt tctcgagtcc
    35161 cagacaacac cattttattc tcaaaggagg aacccaatgg gttcgaagat ctacgttggc
    35221 ggcttgccat attcaaccac cgagcaacag ctgagtgacc tgtttgcggt gcacggggcg
    35281 gtgacgtcgg cgcgcatcat cacggacaaa ttcaccggcc agtcacgggg cttcggcttc
    35341 gtcgaaatgt cgggagattc cgaggcgcag gcggcgatca acgcgttgaa cgggacacaa
    35401 ttcgggggcc gcacgttgac tgtgaatgaa gcccgcccgc aggagccccg ctccggtggt
    35461 ggcggaggac gtggaatggg cgggggccgg cactaagctg cgctcgtttc gctataccta
    35521 ttcaggggct gccgacaggg tagcccctgc gtttttctta ccgtgttgtc ctctctctgg
    35581 tctgcagggc catcaggtta tctgcccctg ttttcccaag attgagattt aggagtgtcg
    35641 ttgcatggtg tcgtttgccg aattgtctct tacatcgttt ctcgctgacc gtttacaaca
    35701 ggccgggttt accgcgccga ctcccattca gagcgcggcg attccgctgg cgttagaggg
    35761 gcgcgatttg ttggcccagg ccaagactgg cagcgggaaa acgctggcct ttcttattcc
    35821 cttgatcgaa cgggccgtca aagagggctg gaagcctgcc ggtcatgcct ccgcgtcgca
    35881 tgccggatct gccagatcgc cgcgggcgct cgtgctcgcg ccgacccgtg agctggccct
    35941 tcaaattgaa atggaattgc gcaagtatgc gccgccgtca gtcacgtcgc tggccgtgta
    36001 cggtggtgtc cccatcgagc ggcactaccg cgccttgcgc caaccgccgt tgatcgtgat
    36061 tggaacgccg gggcgtttgt tggacgtggc ggggacccgc catttggacc tgcgcggggt
    36121 cgaatatgtg gtcatggatg aagcggatca aatgttggat cgcggattcc tgcgcgacat
    36181 tcagcgtatt ctgcaactgc ttcccaccca acgacagacc atgctgtttt cggcaacctt
    36241 ctcgccggaa attctgacgc tcgccgaatc gatgctgaag aatccggctc gaacggcggt
    36301 ggatcccggc gtgaatacgc cgaccacgat tacccacgcg tactacgtgg tgccaagcga
    36361 atcctcgcgg gtgcagttga ttcacacact gctccagtct ccggagtccg gtgatcagtc
    36421 gatggtcttt tgcgatcaga aatacaaagt gaaacggttg gctgcccgtc tgggcggaga
    36481 gccggcctcg gtcggtgcca tcaccggaaa ccactcgcag gcccagcggg aacggaccct
    36541 cacggcgttc cgatcgggcc ggttgcgcac cctggtcgcg accgatgtcg ccgctcgcgg
    36601 attggatgtg ccgtcggtgt cgcaggtcat tcattacgaa ttgcccggca accccacgtc
    36661 ctacgtgcat cgcaccggcc gaacgggtcg ggctgaacga tccggcgcga cgctcttgat
    36721 tctctctccg caggaagagc atgaatactt ggcgatggtg cgccgtttgc gaatccaaac
    36781 gaagcgcctg acgttgcccg cactggcggt actgccgcct cctgctcatg agcctgagtc
    36841 gaatggccaa tccaggcatg atgggcgggg gcgcgatgcc cgaccgcgac gtgccggaga
    36901 gcgccaaaac tccatgcctc aggatgcgag aggcgggcag ggccgtcgag gttggcgtgc
    36961 ggatcgtccc gccgccccgc gccgtggcaa cagttgaaga accgcatggt tcggcaggga
    37021 ttgctggacc ggcatgatta ggaggacgcg tcgatgaaag ctggacggag tctgttgctg
    37081 ggcatgctgc tggccgtcgg aggtcagttg atgggggcgg gcgcctacgc gcaaggcgat
    37141 ctcgccattg ccgacggggt gaaagtgtcg ttggagtaca ttctgacgct tcccgacaag
    37201 tccgtcgccg attcgaatgt cggtcaggag ccgatcatct tcgtgcaggg ggcgcatgaa
    37261 attgtccccg gcctcgaaag ggctctcgac ggcatgaagg ccggacagaa acggcgcatc
    37321 gaggtggcgg cgcaggacgc ctacggtccc tacaacaaca agctacgcca gagcgtggac
    37381 aaagagaagc ttcccaagga cgtcaaggtc ggggacattt tgcaggcgtc ggataaccgg
    37441 ctggtgaagg tcctcgaagt gaatgacaag aaggtgttga tcgatctcaa ccatccattg
    37501 gctggaaaga cgctcacctt cgatgtgaat gtcctcaagg tcgaaaaggg cgatgccgcg
    37561 gacgcccctg caagcaagac cccctaaacg ctctctcttc accagagcgg cggggcagcc
    37621 ggcttgaagc cggctggctc ccgccgctct gtgctgaatc gtcgtcacga gtcctcgaac
    37681 cacatcgatc gttctgtcgc tcagtaaggg cgtgtcccgg ttcttgacgc gttctccgtc
    37741 tccgtgtagg gtcccagcac gagacgttcc agaatggtgt gagggctgac gcgctgttga
    37801 gtaggggcaa ggaaaccttc cacggcttag aggagatcca gtgcagatgg gaaccatcaa
    37861 gcaacgaccg gtggtggcaa ttctggtggg tggcggtccg gcgcccggca ttaatagtgt
    37921 catcggggcc gccacgattc gaagcattct tggtgggtgc gacgtgatcg gcattcaaga
    37981 cggtttcaaa tggatcatgg atggccagac cagcaaggtg aagcgcctgt cgatcgagga
    38041 cattagccgg attcacttca gcggcggctc ctgcctcggc acggcgcgcg cgaaccccac
    38101 ccagaaagtc gagcatcttg atgcctgcct gacgagtctg aacgcactcg gcgtgactcg
    38161 attgatcacg atcggagggg acgataccgc gttctccgcg ctgaagttgg agcagcgggc
    38221 cgccggccgc ctccaggtcg tgcatgtgcc gaagaccatc gataacgatc tggacctgcc
    38281 gcacggcatt ccgacattcg gattccagac ggcgcgccac atcggcgtgg agctcgtgaa
    38341 gagtttgatg gtggatgcgg agacgacctc acgctggtat ttcgtggtga cgatggggcg
    38401 taaggccggg catctggcac ttgggatcgg gaaggcggcc ggtgcgacgt tgacggtgat
    38461 tcctgaggag tttcgacaga agcctcttcg tctgaaaacg ctggtcgatg tcctggccgg
    38521 cgcgttcatc aagcggctca atgccggacg atcggacggc gtggcggtcc tggcggaagg
    38581 tctcgtggaa atcctcgatc agcaggatct cgacggtctg caggatgtgg agcgagacca
    38641 gcacggacat gtgcggctgg cggagatcaa cttcggcttc gtgctcaagc gggcggtcga
    38701 gaaggaattg cgttccttcg gcattaagac caccatcgtc gagaagaaca tcgggtacga
    38761 gcttcgctgt gccgatccca tttcgttcga tatggaatat acccgcgatc tcggttattg
    38821 cgcggcccag tttctcctgg agggcgggaa tgccgcgatg gtgtcgattc aaaacggccg
    38881 gttcattccc attccgttcg gcgacattct ggacccggcg accggccgta ccagggtgcg
    38941 tatggtggat gtggagtctg agtcgtacgt cattgcaagg cggtacatga ttcgattggc
    39001 tccggatgat ttcaatcagc ctcaggcgct ggtccgcttg gccaccacgg ccggtctcgc
    39061 cgcggatgat ttccggaaac ggttcgaata tctgatgggc aaggatctgt gagagagggg
    39121 cgagggattg gacatcgccc catccgctgt gacgtagggc gtcatgacga gtgaagcgac
    39181 accggataca gaaattgctc tcgcatgtgg acagcagcgc gcgctggtct caccctgggg
    39241 cgcggcgtta cgccggtact acctgcttga agacaacggg cgcgaaaccg acattgtctg
    39301 gggctatgca ggcggcgcgc acaaaaaggg tgggcagggc gatgtgctca ttccctttcc
    39361 cgggcgtgtg gcggaggggc gctactcctt tgatgggcag cagttgcagc ttgaacggaa
    39421 cgataaagaa ggcccgaatg cgatccatgg atttgtccgg accctgccgt ggaccacgcc
    39481 gcaggccgac tcggatagcg tcgcgtttga ggtccagctg gaagcggcca actatgcatc
    39541 cagaggctat cctttctcgc tcgcggttcg tgtcgcgtat cggttagaca ggcagggatt
    39601 gacctgctcg ttcgccgttc acaatatagg gcgtgaggct gcgccggtcg gagtcggatt
    39661 tcatccctat ttcacggtcg ggactgcgct ggtggatgaa gcggaagccc ggatccccgc
    39721 ttccgggtac cttgagttca atgaccggct tgcgccgacc ggcaaggtcc tcgatgcgca
    39781 ggataccgag tgggattttc gcgagtgtcg ccggatcggc aacctccgct tcaatcactg
    39841 ctatgtcggg ttggagcgtg atgcggaggg aatggcgacg gccacactgc agcaccaggg
    39901 gagtggccgc aggatcgagg tggtgatgga tcgttcgttt tccgcgatcg tagtctacac
    39961 cggcgatgcg attgtcgccg cgccgcgccg cgcgctggcg attgagccga tgacctgcgc
    40021 gaccgatgcc ttcaatcatc ccgagtgggg gttgaagcga ctgcagcccg atgaaacctt
    40081 caccggccgg tatcgcgtca ctcatcgtct tctgtaaggc tccttgtccg gtgtgtgtcg
    40141 tcctctcctg tcacccgctc cacgcgacca gccatgtgct cttgtccgcg cagactccgt
    40201 tacgacggct tcgtcgattc aacgatgcgg aatcgcactt catccacctg agtgtaggcc
    40261 gaggtttgtt cccctgcgaa taacccgcac cggtagagtc ccgtcgtcca gccggtggat
    40321 ggacgtgaca ggacaaagta accggattgg tcgttggtgg acatgatgag ctgatcctgc
    40381 gccaccgccg gctgcgcgcc agtcagctcc gtgcgttctt tgaaacagcg cgctgccagc
    40441 ggaacttcat cgtacgaagc cgtcaccaat ccgaacacca ggtaaatgtc ctgctgggta
    40501 gcgggaaagc tgattccagg gtccagggga atgaagtcat gggcaccggt gggcgaatcg
    40561 tcccgcatga ctttcttcgc cgggatgacg aatcgaaacc aggcaaaatc ggcatcccgt
    40621 ccgaccaggg aggccgccgt gtccagggtg ttctgtggct tcaagttctc catggccttc
    40681 gcggcgagct ctgcttccga gggaaccacc ggcgccgccg gtgtcaccgg ggcggcgtcc
    40741 tccatctcga ttgccttggc cagttcgggg acatgcggac gaatcttgtc caaccatcgc
    40801 agcagtttat ttttatccag cactctggtt ccgatcggaa gttcgacctc cctcgtcgac
    40861 tgttcaaatg ccacggtcgt gtcgtagagg atcttgaatt gaatgtacgc gtcttcccac
    40921 ttccccaaat ccaccagaca ttgacccagg cgatagacgg tggccgcata gtcttcatcg
    40981 aggggatcaa gtccctccat tttcgggatg atgctgatcg cctctttgca ccggcccagg
    41041 ttcatgagaa tcagtccgag gtgctgcttg gcgagggggt ccatcggatt gaggagcagg
    41101 agttgccgat acatcggctc ggcttcctga tagcgcccgg cctcgaagag cgcgtaggca
    41161 tcgtggcgaa tcatcgacca gtctttgagt tgtttcgggt tcgcgtccgg gctcatcatg
    41221 ggtgcaaagc ggcggcctcg ctccgtcgat ccgtagaaca cggtcagcag attcttggcg
    41281 gacagctcca gcggggaacc gggcgagacc tgttgcaaca cagattgcac ctcgcgatct
    41341 gcgccttcgt aatccaaaat gtcgaatcgt gcgcgggcca tcgccagccg ccagttgagc
    41401 atgtcgggtc ccagctccac cgcctgctga taggacttga aggattcttc cagccggtcg
    41461 agtttccgga gctcacgggc caatcgcaaa tggatcagcg ggttctgggg atcgatggcg
    41521 gcaatacgct gcaattcgat gaccgcttct tccacctgcc cggaacgaac cagcacgctc
    41581 cacttggccc atcgcacgtc cagtgccgtg ggaatactcg ccaggacggt ttcataggac
    41641 tgaagggccg cttcggtatg gcgtgaggtg gccagcatgt cgccacggag ttggtgtagc
    41701 gcgaacgcct gcgggttctc atgaatgcct tggtccagaa tgccgagggc caggtctgtc
    41761 gtaccctgct cccacgcggc tttggcgcgt tgggcgacct ggctttccga gtggaggccc
    41821 tggtcgtttg ccatggccct gggggcgtcg gtcagcgtga cgggcaggag caggaggagg
    41881 aatccaatga tgtgataggt cagccggatg aacggatgcg gcgcgcgaac gggcgaacat
    41941 gtatggatca gcatgagata ggtctctggt tgaggtgaac aagtcccttc ccttgtcagg
    42001 caggacaccg ggcaggcaga ggtgtctcca aacctgcctg cctgtattcg attagagaca
    42061 ccctgaggga gaaggattcg tcgccctcag cggttccagg cacaacgatt gatcttactg
    42121 tacgatggcc cggctctgcg aggtgccccg gacggatccg gctgagggtg gggcacgagg
    42181 cgtatcctgt cgggaggctc ccggtcggcc ggtgagtgtg gttaccgaag cggttgtctc
    42241 gatggagcgg aatcgaatgc gcttaccgca cgttgacgaa gcggccgtga gccatggttc
    42301 gaagctgatc gatgtcctcg tggcgagtga ccgagagcgg cacggtgccg gataactctt
    42361 gaatcaggag atcggtcgtc agcggggttt tgtgatgtaa ggcccggtag agtgcggcga
    42421 ccaccgcctg ctcgatttcc gagccgctga acccatcact ggcgctgacg attttctcga
    42481 ggtcgaatcc cgcgctgtcc tgtttgcgga gcgcaaggtg gatcttccag atggcggccc
    42541 gttcgtggtc gtccggcaag tccacgaaga agatttcgtc gaaccgtcct ttgcgtagca
    42601 gttccggcgg gagggaggcg aggttgtttg ccgtggcgac gacgaacacc tcctgtttct
    42661 tttcctgcag ccaggtgaga aaggcgccga agagccggcg gctcaatccg gcatcggcat
    42721 ctccgcttcc gccgccggcc accatggcct tctctatttc atcgatccag agcacgatcg
    42781 gcgagaggga ttcggccatc tcgatcgcct tccggaaatt cttctccgat tcgccgacga
    42841 atttgtcgaa gagccgcccg gcatcgagtt tgagcaacgg gagttgccac tcgcgtgcaa
    42901 tggcttttgc cgcgagcgac ttgccgcagc ccgggacacc caccagcatg atgccgcgtg
    42961 gcggcgtcag gttgagcgcc ttggcttctg ccgtgaatcc caccttcgcc cgttccagcc
    43021 aggatttcag attcacgaat ccgcccaatt cgaaccggtt gtcttcgagg ggataatatt
    43081 ccaacagccc gccgtccttg atcgcctgga ccttgcgttt caggatggtc tgtacgtcgg
    43141 cagcagacag tctgccgtcc tcgacgaggc attgcgtaat gacctgccgg gcttgatgca
    43201 acgtcaatcc ccgcagggcg cggaggatgg cgtcggattc ttgtgtcgag ggggttgcct
    43261 gggtcggttt ggagccggcg agcgagtgaa gcatgctctg taccacggtg gtggagccgg
    43321 ggcggcgcgg cgacgttctc gtaccgagtg actgcagcac actttggagc atggattgca
    43381 gttcattacg attcggcagc tgcaggtcga gccggaccgc aatctgttca atgtcggacg
    43441 gaaggacgat gggatggccg gtgagcacac aggtcgtacg agaacggccg aaatgatgac
    43501 tgacttcccg taactggcgg gccacagcgg cgtcctgcag gtgtggcgca agatctttga
    43561 gccagaacag ggcctcgacg gtcagtccat tgaggtgttg aaggacagcc agaggggtgg
    43621 ccgtcatctt gctgatgctc tgcccctcgt cctgcctggt gaggcctctg gtaatcgacc
    43681 attcgaacag cggcatgcgt tcctgcgccg ccacggattg aagcaacgcc agcactcgct
    43741 cttcttcgac cgtctcgacg acgatcaggg ggtggcagga gcgaatcagt gtgcggagat
    43801 catgcacgct cgtcgagagt gacataggag tgaaatgcta gcacgagggt ttcaccccac
    43861 caagcaaagg ggcgattgtg accgggcggg aaaatgagcg tagggacaga cgctcatggc
    43921 cgatagctgc tgataaatag tgggaaggaa actggcgaga gtttgttcat attgtgcgga
    43981 tcaaattgac aggtgattcc actgaggcaa caatcacctg agcagaagaa ttctttggaa
    44041 gggaattggg atgacatcta atccggaaaa tctctgcgac gacgaagaca agctggcgcc
    44101 tcggatcgct acattctggc gacgtattgg agctgctgtg gtggatgcat ccctccttgg
    44161 cgcgatcgga gcgtgtctag gttttctgtg gttcgatcag ttggccgcac tcggacgaac
    44221 ggggcgattc attggtggtg ccattgcatt ggtgtatttc ggcacgttga acagccgaat
    44281 cggacagggg cagactctcg gcaaacgtct cctgaagatc cgagtcacag atgccaaggc
    44341 cgcgctggtt agcctacctc gctcggcttt ccgtgcgacg atcttattgt tgccagtagc
    44401 gctcaatgga atgcccgtgc ctgcggggga acatgagcaa ctttggggca ttgttctcag
    44461 tatcttgatt tttggtgtca gtgtggcagg cgtgtatctc tactcctgta atcgacgaac
    44521 aagacaatcc attcacgatt tagcgggtgg aacatttgtg aggaatgcgg agagcacttc
    44581 agagatttat gaagagattt ggaaacctca ttttgcaatt gctggagggc tttgtcttgc
    44641 agtgcttgga gtagtgttaa tgccttacac ttctgagcag cctgagttcg tgcaacagtt
    44701 ggctgaggta cgacgggtgg ttgagtctgc agtgccggat gccgatgttt ccgtgcaggg
    44761 ggggcacaac cagggccaac gcatcaacga ttgggcagac cgctacttca aggatcgtta
    44821 tcggcgtgtc cgcgtgggta aaaccagagg attccgagat ggtggcggac caaattgcca
    44881 tggcgatgtt caagtctttg tccggcgttc gtgatgttgg gcaggtgtcg atagtgattt
    44941 cctatggcta tgacatcatg atctcacacg cacgagcaac aaaagcgttc gtgcattctc
    45001 ttggggaatg gttgaagcga cttaggatcg acgagccaac gagtgccgcg ccgcaagtat
    45061 aatcgtcgat ggctaatgac gatggtgtcg agtgggatag gaatttcgag gatcggcgct
    45121 tcttccgagc aacgagctat attccatcgg ttcttcactc cggctaccag cccaatcagg
    45181 atctccgttt aagttgttga aggaaggctg tgacggagag gatgtcgacc gattcgacat
    45241 gatgaaggct gaggagatcg tcgtcgccgc tcacgagcca tcggacgccg gcggccaccg
    45301 cgcaggtaat gaacttggca tcgtccgggt cgcgtaagtc gggaacgttc gttgggaccg
    45361 cagtcaccgt ttccatgaag gggatcagct cttcttccag tagccctcgt atctcggtat
    45421 tcgtcagctt gaattttgga taggcgagga cgcggatata ttcgtcgagg ataggagcgg
    45481 atacgaccag gggaagccgg ccggattgcc aggcagaaat aagtcgtgat ggtggtccag
    45541 agaatagcaa tgccgagacg acgacattcg tgtcgagaac ggctcgaatc accggcggcg
    45601 acgtcctctc gcccaggcaa tggcgcgatc cacgtctttc atctcgatcc cgagctcttt
    45661 gattttctgg cgaaccgctg tgaggcgcga gcccggttcc gccatggtca ccggccttag
    45721 tatcaggact ccgcccttag ccgtgacatc aaaatagtcg gtttcaggaa tatcttgcaa
    45781 cgctttcttg ggcaatgtca cctgattctt cgatgttttc tttgccaaca tgggggaccc
    45841 tcactacaaa gtaaggatat cttacatcct ggtatcctgg caaggcggct cttcttccgc
    45901 ctaggcgtcg ttctccgtga ggcagcgacc gagaccctgt tgttcggcga gatggtaggc
    45961 atagtgcagg gccgccaggt tcgcgacccg ttcttcagcc tcttcgcggc tgtctgaata
    46021 gatgatctgc agtccgaatc tggccgtcag ggcatccaga aaatccacca tgccgtttcg
    46081 gtggtattgg ccgagccgcc ctccgccgct gttgtattga atagcgcctt cgatcactag
    46141 aaagcgatgc gggcatgcga gaagcggttc gagctcacgg agaaagcgcg gacgattgtc
    46201 ggacgggttg gagaagatgg tgttgaactc ctctacgcga cggcgttcca cgcaaaaaat
    46261 ggccggggct tctgcaatcg catagtcacc ggccggcagt gtctgtgtga tcgttccggc
    46321 gatgcgcacg agtcccttga attcgtacgg cagatgttcg cggctgtcga ccagcatatg
    46381 gatggccggc accatgattt tgggatgact gaaccgggtg acgttcagca tcgtttgggg
    46441 cccgcgcagt tcctggcgcg gtcgtggagc ggcttgcgga cggggcgtcg gcgaggggcg
    46501 tggaggcggt gtcggtgatg agggatgttg tctgccggcg tcgtaccggg cgcgggctgc
    46561 ggggtcgctg agggtctgat aggcctggtt gatcagctgc gtgcgggcct cggctttccg
    46621 gtgcagggtc ggcgaatgca caaagcgatc gggatgccag acctgcatgt gttcatgcca
    46681 ggctcgcttg atctccgcgt ctgacgcgcc gggagagagt tccaggatgg cgtagtagtt
    46741 cgtggtccgt tcgtcccgca tgcggtgtcc ctgatcaatg tgatgaagtg tagcggagcg
    46801 agacggcgaa gcgctagccc gcatgatcgg ggtcggtatg agtcggtgga tggattcagc
    46861 gcgcgcattg cgggttcggc attgtgggaa aattgtcggc ggtgtatcgt aggtcaaggc
    46921 aggttattcc ttctgtagat tccatcggag gcgatgctca tgattttggt taccggcggt
    46981 gccggctata tcggttcgca cacctgtgtg gaactgctca acgccggctg tgcagtcacg
    47041 gtctttgaca atttttccaa cagccatccc gaagcgttgg ctcgtgtgga acggattacc
    47101 ggaaaatctc tgcgcgtgat acgcggggat tgccgtgatc gcgcagccct ggtagcggcc
    47161 ttgcgtgagt cccgagcgac ggcggtgatt cacttcgccg gcctgaaggc ggtgggggag
    47221 tcggtgcagc agccgttggc ctactatgac aacaatgtcg tcgggtcatt gcgactcttg
    47281 gaagctatgc gggagtgcga cgtacagcag ctggtgttca gctcctctgc tacggtctat
    47341 ggcgatcctc agcgtctccc gcttacggaa gaccatccgt tgtcggctac gaatccctac
    47401 ggcagaacga agctcatggt ggaggagatc ttgcgcgatc ttcagcgcag cgatgcctcc
    47461 tggcgaatct gcattctccg ctatttcaac cctgtcgggg cgcatgccag cggactgatc
    47521 ggagaggatc cacaggggac gccgaataat ctcttgccgt tcgtggcgca ggtggcggtg
    47581 gggcggcggg aatgtttgaa cgtgtggggc aatgattatt cgactccgga cggcaccggg
    47641 gtgcgggatt atattcatgt ggtcgatttg gcactgggcc acctcaaggc gctggaagcg
    47701 ctcgatcggc tggaacgtcc gggagaatgt ttgacggtga atctcgggac tggaaatgga
    47761 tacagtgtgc tggagatcgt gcgggcgttt gaggcagcga gtggaaagcc ggttccctac
    47821 aaaatcgccc cccgcagacc cggtgacgtg gcctcctgtt atgccgatcc caagcaagcc
    47881 ttgaagtcgt tagggtggga ggccgcgcgt gggctgaatg agatgtgtgc cgatgcctgg
    47941 cgttggcaaa gttcgaatcc aaaggggtat gcgggctgag tgttcagcgg ggcgagcgct
    48001 gtgagtcggg ggatcttcgg atgaaaacga tcggcctaca gagtgagcca gtgctcgatt
    48061 gtcgacaggt gagcgttctg catatcacta cccttctact ccgggcgggt ctcatcatcc
    48121 tggcgtcccg gggtgcgtca tgggcaatcg gtgccggggc ggtcgcgtgc gctctcttat
    48181 tcgtgccggc ggttcatgcg aaccaccaag ggcacgtaag ccatccggat ggcgaggctc
    48241 atatcacggt gggagcgggg tactcgatcc ctggcgcgtc ctttcagatt ttcatggggc
    48301 caggcaacga tgccgcagaa gagaccggtc tgactcaggc agacaggagc gccgccgttg
    48361 aaacggtgag agaagcgttc gctgttctcg tacaacatcg gaacgactat ccgcgtttcg
    48421 atgaaagcct caagaaagag gcactggcca gggtcgtaat tgagacgacc gtggtgaatg
    48481 acgagggaaa agcattcccg ttcctcgtcg cccgaaccac agagccggga cgggtcatct
    48541 tgctgatcag cgcgtcgtca ctgaaggaga aggggtatct gcatcacccc gataccctgg
    48601 ctccggtgct ggcaagagaa tttcaatggg tggtcagcaa ggcggacacc gcgccgaagc
    48661 caaaaatggt tgcaggtgaa agagcgttga agtcggcgcc gattcgcacg gaccaggata
    48721 tcgcggccct ctccggggag gagcgggtac gtctccttca gcagttactc ggctcgtacc
    48781 tgcgaacggt agatgatcag agaagcctcg acggccagtc ctactatgaa gtccgcagca
    48841 ccgcactggt cgcgccgact catccggatt cgacgaccaa gctctatgag atccgggttc
    48901 gtgaggccct gcagcgcatc gtccgggaac aggtgttctg ggaacgtacg ccgaaggcag
    48961 tcagaagctt actgaatggg aaggtgtgga ctgtcgcgtt cgtaaacatc gaccaacgtg
    49021 attgggccac tcggaccagg gtactccccg aggagaaggc cgtcatagta ggagcgcgcg
    49081 accaacgcgt tcaaccggcc gcaattttgg tgaataccta tcgaacggct gcgcccgatg
    49141 acccgtttta tccggacacg caggggctgc ccatgggcgc gctctcggcg gagcagttgg
    49201 cgcgcgtgat cgccctggag attcagcata atatccagga gaaatcgatg gccggccacg
    49261 tggcgcagga tgcacttacg gctcctaaat agggatggag cgaaacgagt gattgagtat
    49321 cgtaccgtaa tcgactggcc cagcgtctca ttgcttccgc ttgacccatc gctcccgctt
    49381 gtcgcaccac gcccgctctg ttcatcccaa gcgtcccatc ctactgcaac gagtcttcct
    49441 ggctgaccgg tttggggcgg cctggtcggc gcgaatcgat caatgagtag aatgtactgg
    49501 catgcatacg tagtgcaaaa ttgcatatgc gtagtggttc gctctacgta tggggcagcc
    49561 gcagtatttt tgcgcatatt ccagagagct tttcttgacc tccgtgaagt ctgcgtgtag
    49621 gtttcccccc tgatttttgt gagagtgcgt tgcgggatcg ccatgtatcg gccacgtgcg
    49681 gtgtggacga agccctgtcg cccctgcctg gctcaacgta ttatccggac ggcatcgcgc
    49741 gacgttgacc ggtctgtgtc ttgcgggatc aggtgggcat ggcaccgctc ctacgcagca
    49801 tgtatcgcgg ctgtgtcgcc atgagtaggt tcggaccctc tcccccgaag tgaatgtgcg
    49861 gctgacgacg gggcgcagct caggcttggc aagacggatc tgctgttcaa ttgaggagga
    49921 gacatgtccc gttttgtgtc tgcgctgtgc ggggcagtgc ttgcgcttct ggctgttctg
    49981 ccggggaatg cggcggcagc accatcgtcg ggctcaagtt gggaaccgat gcggatcatg
    50041 catttcgtcc tgaccgggac ggtcccactg gagcgtgcgc gcgagtatgt gaaccaggct
    50101 caggcggccg gtttcacggc tgtgcaggtg gtgctcaccg atggcgtgag gctcgatcat
    50161 gccccgtgga aacccgtgaa gggcgcgtgg actaaagccg agtttctctc ctgggctgcc
    50221 tacgcccgcg cgcatggatt ggacgtgatt cccgaggtca aactcctgac acatcaggag
    50281 cagttctttc agaagcatta tcccggtttg atgttcaatg ccgtgtctta tgatccgcgg
    50341 aaggaagggg tctatcaggc tgtattccgc ctgctcgacg aacttatcga cgccctgcat
    50401 ccccgggcga tccatatcgg ccacgacgga gcatttggat ggacggtggg ccaggtggcg
    50461 aaatggctga agttcggtga ggtcatggtc ccggcggacc tctttgcccg ggatgtcgaa
    50521 cgcattcacg gctacctgac tcaaaagggg gtggagacct ggatgtgggc cgacatgctg
    50581 ctcgatccgg cggagttccc cggcgcctcg cccaagcatc tgcatggggt ggcggcaggg
    50641 tatgggaaag cgcttcgcaa tcggcttcca cgggatatcg tgatgtgtga ctggcactac
    50701 ggcgacgagc aggggagttt tccctcgatg gccgtgatgc agagcgaagg gtttcgcgtg
    50761 atcggagcca cctggaaacg ggaggagacc atgcgcaatt tcagccgcta tgcgttgtcc
    50821 cggcacgcct atggtctgat ggcgacaacc tggttccacg tgcagcggaa cgaccgggac
    50881 ttggtcgact ggattattcg ctcgtccggt atgttattcc gtaaccccga tgcggcggtg
    50941 ccgccgagac ctgcttcggc tgcccctccg gaaggacatg gctggacggc tccataacgg
    51001 cggggaaagg tcgctcttga gtgggaccgt cagggccccg gcgcgagggg aacgcctgcc
    51061 ggttccgctg tgtcatggtg agccgggcta gcggccgatc tgtttaggct gccccgcttt
    51121 ggctagctgc acattcacgg tcacgactcc ctcattgaga ttacgatagg ggtaggcaga
    51181 ggtgataaga gggagggtat cggcttgttc ctgtgtggtc acgagggtga gcgccggcac
    51241 ataccggcct gaagcgactt tgaccccgat cagcttcgct cccggttcga ccaccacgtg
    51301 gtcaccgagt tcagtgcgaa acacaagggc ctgcatgccg acaaaggtat cgttaccgat
    51361 tctggcgggg ccatgcacct gcgattggtg ggccagtgag acgcgcttgc cgatatagac
    51421 ggaataggtc ttgcccgaga cctgcacttc gttctccggc aattcatggt ccccttcgaa
    51481 cgtctccagt ccatggatga cgaccccgtc ctgcacgtta ctgtgatccc cgacataaat
    51541 gttctgtcct tcatcgccgc ggaccgaagc gcccggtgcg atgaaaatca actcgccgat
    51601 agtcacggat ccaatgaccg ccgccagggg atgaatgcgt gccgaggcgg caatcgtcgg
    51661 cacagcgacg tggggattaa atgtggtggg aacgttgggg gctacccagc cgaccagcgt
    51721 gatgccgatg aggagcaggt tcaggctcag gctgagtgag aggggcaagg atcgaggcat
    51781 caagaattct ccgggaaaca cgggcgctca cccctgtatg gatgagcctg tcgttgtgcc
    51841 ctgcatcacg atggagtgaa cctcagcgta caaggcttgc tgccactcgg cttgcaggcc
    51901 cggcgtcatg tcggtgtcgt attctttgac ggtggctcac gctgttgatc cagtattcgt
    51961 cgcgctggtt cacgcctctt gccgcatagg ctcccctcgt ctccatgtcg gcggatggcg
    52021 ccttaaactt gaggtacgcg tcgagtctcc tcatcccggc actcgggagt ggaccggacc
    52081 ggcctggaac acgtagaatg cgcctccgcg gtgctcaccc cctccacccg tgagggcgtg
    52141 ggaaacgaca gggttgaaac aacagtgggc tgactaggaa gggatcgtca tgagtgtgaa
    52201 atgtggaatc gtcgggctgc cgaatgtggg caagtcgacc ctgttcaatg cgttgaccaa
    52261 gtccgggatc gcagccgaga actatccgtt ctgcacgatc gagccgaata tcggcatcgt
    52321 ggaggtgccg gatgcgcgga tgcaggcgct ggcggacatc gtgaagccgc agcggatgca
    52381 gtatgcgacg accgaattcg tggacatcgc agggttagtg gccggagcct cgaaagggga
    52441 aggcctgggc aaccagtttt tggccactat tcgcgagacc gacgggattg tgaatgtcgt
    52501 gcgctgtttt gaagatgaca acgtcgtcca cgtggcgggg aaggtcgatc cgatttccga
    52561 catcgcgacc attgtgacgg aactcgcgct ggccgacctc accacagtgg aaaaagctca
    52621 ggagcggaac gtgaagctcg ttcgctcggg cgataaagat gccgcaaagc taggcgagtt
    52681 gctggtgcag gtggccgcct gtttgaatga ggggaggccg gctcgcacct tgaaactcga
    52741 tccggcgcag cgcgccttgt tgaagccctt gtgtctgctt acgatgaaac cggtgatgta
    52801 tgtggccaat gtggctgaaa aagggtttgc caacaatccg ctgctgacgc gggtggaaga
    52861 atatgctgcg cacgaggggg cgccggtcgt cgccatctgc gccgcactgg agtccgaaat
    52921 cgcggtgctc tcagacgaag agaagcggga attcctggcc gacatcggca tgaccgaacc
    52981 ggggctgaat cgactgatcc gcgcggcata ccaactcctg ggcttacaga cgtattttac
    53041 cgccggcgtg aaagaagtcc gcgcctggac gattcacatc ggcgatacgg cgccgcaggc
    53101 cgccggcgtc attcacggcg atttcgagaa ggggttcatt cgcgccgagg tgatcggcta
    53161 caacgacttc atcgcctgca aaggcgaagc gggggcgaag gaaaaaggga agatgcggct
    53221 ggaagggaag gagtacgtgg tgcaggacgg cgacgtgatg cacttccggt tcaacgtcta
    53281 gcggcgctct tttaccatgt tacgaggcag ggtttgcttg cctgatcgga gatccaacgt
    53341 gaacgaacaa gacacacagc gagctgtagc gattctcaac agaattatgg aacatgagtt
    53401 ggccggcgta gttcggtaca cgcactacgc gctgatggtc tacgggtaca accgtatccc
    53461 gattgtgtcg tggctgaagg gcaatgcgga tgagagtcta gcccatgccc acaaagccgg
    53521 tgagctggtg actctattgg gcggccatcc ttcgttgaag atcggtaccc tgctcgaaac
    53581 ggagaagcat gatgtggggg atattctgcg ggagagtctg gagcacgaga aagccgccgt
    53641 tgcctcatat tacgaattgc tgaaactgac ggagggcaag tccgtgttgt tggaagagta
    53701 tgcccgcgag atgatcgtcg gggaagaact gcatctggac gaagtgaata agatgctccg
    53761 caagtcgggc gatgtgcagg ccttcaagtc ctaactattc acgctgcctg acggcacagg
    53821 cctggtccgg agttccggac caggttttcg tcatgatcca ctacgccgat tgctgccgcg
    53881 cattgaagcc ggccactcac cgttacccct gaccctgcct gtatgtttaa cgtgattctg
    53941 taccagccgg aaattcctcc gaacacgggg aacatcatcc gtctctgcgc caacaccggt
    54001 gcctcgttgc atctggtcaa gccgttgggc tttacgctgg aagacaaaca gttgctgcga
    54061 gccggactgg attatcacga attcgcctcg atcacggtgc acgaggggtg ggccgagtgc
    54121 ctagcacgca tggaagggcg ccgaattttc gccgtatcca cgaagggcag acagcggtac
    54181 gatctggttc gttacatcga aggcgatgcg tttgtctttg gcccggaaag ccgtggattg
    54241 ccggcggaga ttcttggcag cgtccctgaa cagcagtgcc tgcgcgtacc gatggtgccg
    54301 ggaagccgca gcctcaacct ctccaactcg gtcgcggtgg tgctctacga agcctggcgg
    54361 caggtcgaat tttggaagtc tgtgtaaaga ggtgaagcgc agatcgccta tggcttatag
    54421 acgataggct gcagcagaaa aaacagaatt cctctgctcg tgctggcgtc gctcaggcgc
    54481 tggtctgtga aaatggatgc agatggctca agcgcacatg tttttgcgcg taggcctcac
    54541 caacacgttg aatatcaggg atgtacttca ccgagtccat cacttgcaca tgttctgctg
    54601 acaggtttcc gagacccaag gtttctaagc cggtctggct cacgtcggga tcataacgca
    54661 cataggtgaa ctgttttgcg ccggtccagt tggatgtcgc tgcgtctgcc gcaagcacca
    54721 tgtcgccgat ttcgcggtcg atctgcgcac cgaaacggca ttcgcccaat aggcggcagg
    54781 ccatgtccca tccggcgcag gccgcattca tcaacgcgcc gggtacatgt ttcgcgtgat
    54841 ccagtaacca gaggtcgtcg gcctgcagat ccaggcgtgc tttggcggag caaccggtgc
    54901 cgaccgagac gatcaatagt ttgtctgtgc cggtggacca atggagcttg tatggagccg
    54961 ccgtcgccat ttgaaaggcg agaaacgacg ggttgttgta ggtggtcact ccgccatcga
    55021 cgaacacgaa ctgatactcg tccggggtgc cgggcgcgaa ggtcaccact tcgggcggga
    55081 agaaggtcgg cgcggcggtg ctggcgcgca ccaattgcca gagcggcaaa tgaagattgc
    55141 agtccttgcg atcgagttgg ttgtacttgg cttttgggtt attccagatc ggccagggtg
    55201 aatccgtgct gtggttgcgc aataccatca tcagcagcgt gcgtaatccc gcatcgccga
    55261 gggtggcagg gggcgcgccg tcggggtagc ccagcgcccg gttgagttcg acgcgaagtt
    55321 ttgcggccag cggttcgtcg ttgtagctat agcgcagtcg cttgaagacc gaggccttgt
    55381 cgaacatttg ttcgccgctc tcgacataga acgtccggat cttattcatg gacatgcccg
    55441 ccgaaatgca ggcggcgatg atggctcccg tgctggtccc gcagacgaaa tcaaaccagt
    55501 cggccaggac caagtcaggc ttgcccagtc gcgcgcggag atcggcctcc aatttcgcca
    55561 gaatctccac cgtcatcagg cccaggattc ccccgccgtc acagacaagg attttcttgg
    55621 ggccggaggg ctcaagttgc gcttttctct gcgcattcat ggtgtttcct ccaaggtatt
    55681 caatcgcatt tggaattgag ccctcaacct aggatgtttc gttcgcgttg gcggcgccag
    55741 gcttctattc tggcgcagga ggcccgggca ggccacggca ttgaatttcg ggaggtatgg
    55801 cttttgacac ccattcatgc cactgatcgt cgctcatatt cctatccaac ttggaacaga
    55861 tgatgtcggc ccattgatca ggtccgggcc agaggagaat ggtcgatgca tcagcagtca
    55921 cgaagaactt tccatcgggc gccagcgcta ccgcctgaat gtcgctgctc tgatggccgg
    55981 ggaaatgtgt ggcgatctgc acatgttcgt ctccatccca cagttcgaga cgtcctttgc
    56041 gtccccctat caaaatcagc ttcccatctc cgctcgtgtc aatcgaggtg agcggtgtag
    56101 agtcacgaaa gcccaaccgg gacttgaatt catcatgctt tcgtaggccc tcgacggttg
    56161 gttgatcaag gatggtggga tgccattcca ggatacggcc atctgctcct gccgaaagta
    56221 accatcttgc gcttccccga gagagtgatt ggattgaatc gatgtgtcct tggagtatgc
    56281 gctcagattc cggcttagca acatcaagca gacgcacgcc ggcggaactg tctcctacgg
    56341 caaggtactt gccgtcggcg gtgaaactga gagtatggag ctcataatcc tcctgcgcat
    56401 cgatgggcct ctcataccgt cgaatgttgt tctcggtcgc gatatcccaa agacggattg
    56461 tgccatccct gcttcctgca gctaccaact gcttatcggg cgatacgaca attgatcgaa
    56521 taggctcggc ctgatcttct atttccgtgc gaatagcttg gccagaggta gcgtcccatc
    56581 gtttcagcag gccgtcgtcg cctccggaga caatcaccgc ccggttgcca atgtgtaaga
    56641 aagccaggct gcggatggct tggtcatgcg cgtgccaatg ttgatctgag ggtaaggtaa
    56701 taggctcaaa atcaggctga tgaaatgacc tccagagata tatggtgccg ttgtcatctc
    56761 cagctacgac ggattcgccg ttcgggccaa tcgcgatggc gctcacattc cctggtagtg
    56821 gaacagcctg ttgcgcatcg gatgatagct gcaactgtgc cagggagaat ccgggagttt
    56881 tgtggcccca aatgcgaatt gaaccatcct gtcctgttga cgccagacgg gttcctcccg
    56941 gattaaaggc tatccccata gcgatcccgc cgtgcccgac aagctctcca tcttgaatca
    57001 gcgagaattc tggcgtactg ggagccgtgg ccgactttcc cttcgtggcc ggattgctgc
    57061 agggagtcac attttctaag agccgaaaca cccgcacata cccatcggcg cttgcggctg
    57121 cgatcagccc atctggactc aaggaaaaat tgacaatcct ggctggctgt tgcttgctct
    57181 gctgtgtgcg acaacgttct ttgagattcc acactatgat gagtccatca tctccggctg
    57241 aaatcagaac gttcgggtcc gtcggggaaa atgtcacttg ctgcaccgtg gccccatgtc
    57301 ctgtcaattc cactggtttc tccgttggct tgtcacggtt ccacaatctg atgatgccct
    57361 tctcatcacc ggaaaccacc aaacgattat tagggctgac tgccaccgaa atcaccttgt
    57421 cactgtgtgg aagttcactc atcgtcccgg tgttgaggtc ccactggcgc accatcttgt
    57481 cgcttccgcc ggagacgaca aactctgctt caggtccacc aacataagcc acgctataga
    57541 tgtcgcccgc ttcatccttt ttgaaccggt gtagtgtttt gcgctggagc acatcgaaaa
    57601 cacagacgaa accgttccct atggtttggt cattacttgt aagatagccc gccgccaagc
    57661 gcgtgccacc gctgttatag gcgagggtcc acacggacag ggattcagcg gtcggttttc
    57721 cacaatccag tttgcccatt tcacgatacg tttcggcatc gaggagacgg atcgtgccgt
    57781 cctccccacc gacggcaagt gttttaccgt cgggactgta tgcgagtgca ggcgttggca
    57841 cgaggccggg aattgagacg gctttacgta gatgcggggc actttccata accgaatgga
    57901 gcgcagtcag gcttgcggct cgggcctgcg acaattttcg atcaccttgg gtcaagcggt
    57961 aggcagcgag cccccgatac atcatctgct ccaacggctc catgcctgac gcaagtgcca
    58021 accttccttc ggcgaggcgc gatgtgacaa gggccactgt tgtattggct tcaatctctg
    58081 cccgcttctg tgcctccgct gccgttttca tctgctgctt gatgccccac ccggctaaga
    58141 tcacggttat gatgacggct gcggtggcaa ggttgcgcat gaggttggcc accttgaggc
    58201 tacggcgggc catgtcccgt tcggcttgca attcttcgct tctggctcgt tccctatcac
    58261 gctcttcctg ggccagctta gccaaccgtt cttgttctgc ctggtgctgc ttgatttttc
    58321 gctcgtctct ggccttccgc accacgggaa cgaggcgatc gtgaattaac tccacgcgcg
    58381 tggtgtcagg gtgatggacg agtctcagca aacgatgctg atccgtgagt gtgtgcaact
    58441 gtttctcggt gagcaacttg ttgccgagcg cttcatcctt tgggaaagcg ccgcgaaagt
    58501 gatcgccttg gatcaagtag tcttcgatga acagtgctgc atcaggcaga ccctttaccg
    58561 cagggtcctc gagggcttcg cgatagaatc catctaaaat atcctgcccg gtatgttcaa
    58621 ccagtgtctg gtcgattttc gcgccgtcgg cccgtcgagc gttgagcctg gagcaacaca
    58681 agctgagcag cacgggctca atgactatct cggacatatt ggttgcgtct gctgcgtggt
    58741 ctccttttcc gactagatcg acgatcaatg gtgccacatt agcgtcgagc acttcttcac
    58801 cggcctgtgt gaccgcttcg atcgcacgct cacggggcat cgggctcagc cgcaagtagt
    58861 ttttcagcaa cgaaggagct ttctgttccc acgcttgtac ctctggcagg aaatcctctc
    58921 gaaacgacag gacaatgcga taacgctggg acagaaggtc cagcgaagct ctcacggtcc
    58981 ccgctccttc tttgatgact tcaggcggga tgcggttctc tatgaggtcg gccaaattgt
    59041 agataacatc tttgatcagc tccatatcgc tgcccttctg cgaaaacagc tcttcaaact
    59101 ggtcgaacac caagacggga gtaaggagaa agttgtcgca gctccaaatt ttcaagtctt
    59161 tacgatggag gtactcccac aggctgcttt cgctgcgggg ctcagggtac tctgccttct
    59221 ccttgtccaa ttcttctctg aggcgtcgca tgacttgttg aagcggcggg tccctcatga
    59281 catccacgaa ttccaaccgc acatggaccg gcaaatagtg ttgctcgcgc agcttcggat
    59341 agagcccggc ctgcaggagc gacgttttac ccagtccaga tttcccatat acaaccgtca
    59401 acggcgacag tcgaatgagc cgaaaaagtt cctcagcttc ttcgtcccga ccgaaaaaga
    59461 atgcgctcga agcctcgtca tacgcagcca agccaggcca tggattatag gcattcagat
    59521 tggttgaact ctccgacaat acaatcacga tggcctaccc tcatctcgaa acttgcgtac
    59581 caacttcttc agctttgcct ccaggcgcgc gtccggtacg ccctctggcg cgtgggcaaa
    59641 gtcgaactga ttatcgtctc tccatttctt tacagtttca aaggtataga tcgtcggtgt
    59701 gaagtcgtca tcaacaatga tcgggaagat gaactcgcgg ttcattggga ggcgagcatc
    59761 tgcccggtcc cactctttga acacaaaggc ttcttttcgg ctgtcgcaag atttcgacag
    59821 caacggcaaa aaatactcac aactgcggat cccgtccaaa atgcggcgtg ggaaattctg
    59881 ccctggttcg atgctctggc ggtcgaacca cagctcgctt tcggtgacgc caagcttgcg
    59941 tagtgattga tacagcattt cggctcgagg caggtccgtt tttcgcgaat agctgatgaa
    60001 aaacatcgcc ctgcagggga ccgactcttc cgccgggcgc tctggctcct gcgcggcagc
    60061 ccgatactcg tccatccaac gtcggtgtag ttcagcgacg aattcaaccg gtgaaaggtc
    60121 cgagagaact tcagtgtctg caccgtagct gttgaggaaa caggtgaagc tttcctccgg
    60181 cttcagctgt tcgatcaacc atgaacgcct gtatgttgaa gcaagccgtt gtcggttggt
    60241 tgcgcgcaga aagaaccgac tcaaccattc cgggaaatta cacccgatca tcagcagact
    60301 acgttcttgc aatacaccga ggaatttatt gagtatccgg tgaccatcaa cgatcacgtt
    60361 atgggcatac tcaaggacat cctcgtcgtg aatcgcgaag gacgcatcgg catgcgcttt
    60421 tccgaatagg tacaatacat tcacttcgct aggtccttta tcccaatcct tgggcagatc
    60481 attgcctccg cgatccgaca tattcgggga atgtatgatc tcgttcacgg cgcaacgccg
    60541 tcgtctcagg ctacgggcga gcaggtcgtc cggggtcagc gtcacgaaga ggcgaaagtc
    60601 ggcaatagct gctagctgcg tgattggtgc cggtatgaca aagtcttcag cagtgatcac
    60661 ctcgcggatc actctactta agtcggcata aaggtcttgc agtttggctt ctcccttcaa
    60721 gtgtgaaacc acttcgttga tttctcggaa aagtggaagt gcaatctctc cgggtttttt
    60781 gccataatcg tgtaagagac gcgctgcgac ctgagcctgt aatgatgtct gcccgtctgc
    60841 tccaaccagc agtcgagatc cgataatcgg aataacaccg ttgtcccgaa ttttgtacat
    60901 cagtctcttc catcctgcct cattcatttt ttccctcagt tcgcaaggcc ttagaactta
    60961 cggctatcca gccacgtcac ctgccccgtc ctgcgtttga cctcctcgta catgtgggcg
    61021 gtgccgcccg gtccgtctcc gccggcgccg tcccagaggc agaggaatcg cacgcgggtg
    61081 acgccctggg acagggcggt gtagagcagc caccggttgc agcgttcaaa cggattcacg
    61141 ccgtcgggca gggggccgag ttcgtccggc atgatgcgcg gctcgtcctt caggcctgat
    61201 ttgaggcggt agaaccgctc acgccacttg tcaccgtcgg cagagggaag gatcgagcgc
    61261 tggatgaatt ccggctccgg gagcggcagc aaaagttgta gtcgtacatt tcgttgccga
    61321 caggcttcga gaaacagcaa atccccgccg ctggccccct gggtgagcgc gaggtcctcc
    61381 ggtccggtgc caagttgatc caaggcctcg gcgattttct gtgcggcgac cgattccttc
    61441 tcggccggga accgcttgct ggggcggtcc ggcgcgtcga tcatgtggcc gctgaagagg
    61501 atcacctgtc gcggttgcca gtcttcaggt ttttgaagtt tctgcaaagc gcagttgaag
    61561 gtcgcaatgc cggcctcgac attcttcggc ctgaagccca ggtccttgag cagttgcagc
    61621 tgggcacgac tggagttgag cgcaaaccag tccttgtcat tttttgccag ggcttccttg
    61681 taagctgcct gtgccgcctc cggcgtaccg accagcacct ccagttcgcc cagggtcatg
    61741 ttcgaccaga agcgttgctg ttcatccggc tcggactccg ccgcaaatcg gacggccccg
    61801 gccatggcgt ccatttcgcc gtcgtagcgc ggatctttga tgatgaggtg gcgatggagg
    61861 tgcatcaagg tgagcgcgtt gatgccggag tagtaatgac cggggttgcg gcgataggcg
    61921 gtcgcgtagc tgtcgatggc ggcgcgcagc agggcatctt cgtaggccgc ttcgtcttgc
    61981 atctgctccg ggctgttgcc ggaccgccgc caggcggcga tccaggcatc cttatcgact
    62041 cgaccgagca gggcccaggt ttctgggtct tgcggataaa gctccaagat tttccggtaa
    62101 tgcgcacgcg cacggtccaa cgaatagcct gggcggccgg ctaaggccag ccgttgcagg
    62161 cagataccct gctcacgcag acccttgaga ttgcgcggct cgaccgccag gcctcgttcg
    62221 agctgttcca aggcaaagcc gaagcgttcg gccttgcgca aggcctcgcc ggccttgatc
    62281 caggcctcgg ctcgaaacgc cgcgatcggc gcctcgtccg caaggaccag gacatcaccg
    62341 atgtgccccg cttttcgcgc caaggcgatg cggcttgccc atgtctcatg ctgttgccag
    62401 aattcccgca cgtcgccgat gcggagcgac ttccagtccg gttcctggag atttggcatg
    62461 agctgataga ccggactcac cttgcggccg tgccatgact ccatcgtcgc tgtgaccatg
    62521 tcggcgagat tcttcttgtc tgcctcgact gtcgcgggat ccggcccacg gtccttgatg
    62581 ctgtagcgga gcttccgatc ggtatagagg tcgaaggcgg tgggcactcg tccgccgcag
    62641 gtcaagacga cgccgcgggc gcgcagcgca tgacgcacgc ccagctcgta ccagacgttg
    62701 ggattgtcca aggtgaggtc cgccacgacg aggtcggcga tgaggagttc ctggaacatg
    62761 tcggtgcgta tgtcaccggc gcgtaattcc tggtcggccc gaaagggttc cagccctgcc
    62821 gcttccatgg ctggcttgat cagctcagca tagacacggt taaaatcaat ctcgtggccg
    62881 tgactgtctg gcttcacgcc gtacggcatg gcgacaaaag catgtggttt catgcgagtc
    62941 tccgatggcg ggggaaaata tgttcgattc gaatctcacg tgctccagct tgcgtaccat
    63001 gggaaattgc tagaggcgca tcatactcct cctgcaactt tctgaacatt gcgatagttg
    63061 atcgaaggtg atgcatttcc atcccacacg ttttgaagtc gaacagaagc gacattcatt
    63121 tttgtggggt acgccgttat gggtggagat atgtgtcggt tgcggagagg catgtggacc
    63181 tctagcagga tgcggaaaaa gtccgccagc ggcgttctcg catcgctcgc cgatccgtga
    63241 agcgtgaatc gtgaagcgta tctcgtgaac atgccacagt cgattctgaa acgagagacg
    63301 aacaacgctt cacgaacaac gaacgaaggg aacggccttt ttgcgcatcc tcttggctat
    63361 tctgatacca accggtcacg tgagctgccc gcggcgtatt gtgcataaat cgagtttttc
    63421 cgcagcctgc tcgtggcttg ccatttcagg ctcaacgaca gagttcccag tctcaagcat
    63481 caatagaagg agcaaacgat gtgccatgag agttatggag cgtcttttcc agttttataa
    63541 ttgggatacg aaatacgtgt atttttggaa ggaggagcgc aagggaggcg ctgtacctaa
    63601 atgatggctc ctttggtatc ggaagagata cagacttcga tcctccctgt atgcagatat
    63661 cccgcaagca ggccgtggtt gaacaaggtc gcttcctgtt ccataataac caagcgaaaa
    63721 ggagggagaa cgaggtttcc atgaagacgt ttcaactcat caccgttgcc atggcgacag
    63781 ttctctgtgt gagcctctcg ggttgcggaa gtggactacg cgagaaaaag atcgagtttg
    63841 tgtatctcgc gctcggcgcc agtgacgcaa tcggggtagg agccacgccg ttgactgaag
    63901 ggtacgtgtt tctcgtcaag caagaattgg atcggcggat gcccggggtc ttcttgatca
    63961 atctggggat acccggggcg cgcatagatt tgatcaagga gcaggtgcgc gtcgcgaagc
    64021 aactcggcac caaggcgcat ctcgtgacgc tctggaccgg cgcgaacgat ctcgtgcacg
    64081 gtgacgaccc gaagaccttt cggcaggatc tgcggtttat tcttcaaacg ctccgggaag
    64141 aggtttcgcc acacattgtg atcggcaatc ttcccgatct cacccggctt ccccgttttc
    64201 agcgggcccc tgatccacgc gtgaccacgg cacgggtcaa tgcctataac gaagccatta
    64261 cgcaagaagc gcgcgatgtg aaggcgttcg tcgtcgactt gttcgcgcaa ccggttcgtg
    64321 aggaactcat cttcgacgtg gatggttttc atccgaatga cgccggccat cgcgagatcg
    64381 cccggctgtt tctgcaggcg atcctagcga agtcgggtgt gaaatagagc ccgtatggca
    64441 caaacaggat acaaaacgct ggccgaagcc attgaggggc tccgagaaag agggtttgca
    64501 gcgaactttg aactgctcaa caagacgttc ctggcggtga atagtggccg gacattcacg
    64561 gccgatcagc tcacgattgt tgagcatcat cggtttgaag gagccagtga tccggatgac
    64621 atggccgtgg tatacgccat cgaagcccgg gacggaactc gggggaccat tgccgatgca
    64681 tttggccccc aagcgaatcc tgaccttggt gcgtttctca aaaatgtgaa tatgcacgaa
    64741 gatctgtagt gtcccgtcgt aaccgagatc ctttccggca gacactcatc gccattgttt
    64801 caaatctcca gcaccagtcc gatttcaacc acctgttccg ccggtaggtg gaaataggaa
    64861 ctggctctct gggtatttcg tgacaggaag acaaatattt tttcccgcca gattgccatc
    64921 cccggtttcg gcgtcgccaa aaaggtcagt ctgctcagga agaatgtggt ctcctcgaca
    64981 gggagggtat ggccgcgggc tgcgaggtag gtgagtacac gtggaatgtc cggcgtttcc
    65041 atgaatccgc atctggcgac aacttgaaac aggttttcct ggatggtggt gatttccagc
    65101 gggcctccgg atggaacgta gggcacaggc tctgtgcgga ctgtgaggat atagacctgc
    65161 tcgtgcagag atttgttgtg ccggacattt tgcagcaagg cgggcggggt gatgtcggga
    65221 tgttgagata aaaatacggc gcgcccggcg gttctgcatt gcacctgcga caagacgtcc
    65281 cgggcgaatt gaagcaaggg aggaaactgc tcgcgcaagt ggtctgccac gatcgcgcgt
    65341 cctcgcgccc aggtactcat gacggtgaac acggccgcgc cgagcagcag cgggaaccat
    65401 ccgccatggg gaattttttg ggcattcgcg agaaagaagg cgagatcgat gagcaggaag
    65461 cccccgactg ccatggctgc cggtagcggg ctccattgcc aatgggcctg gaccacgcgg
    65521 tacatcagga gggtcgtgat gatcatggaa cccgagacgg cgatgccata ggctgcggcc
    65581 agattactgg acgatccgaa ggtaaggatc agaccgacgg tgccgatgaa catgaaccaa
    65641 ttcaacaggc caaagtagat ttggccatgt tgctcggcgg acgtgtgggt gatccgcaac
    65701 ggcggcagat agcccaattg aacggcttgc aaggtcaacg agaaggcccc ggacagcatg
    65761 gcttgggatg cgatgacggt ggccatggtc gccagcgcga tcaaggggta cagcatccag
    65821 tcgggggcca ggaagtaaaa gggattggcc agagccgccg gggtccgcat gaggagcgca
    65881 ccctggccga agtactggag cagaagggcg ggcaaggcca ccacaaacca gccgagttgg
    65941 atcggtcggc gtccgaagtg gcccatgtcg gcatacagcg cttcggcacc cgtgaggacc
    66001 agaaacacac tgcccaacac caggatgccg actcctgcat gccgaatcaa aaactggatc
    66061 gcatacagcg gattggcggc acgcagaacc tccggggttt ggatcaaact gtggagcccg
    66121 agcagggcca tcgtgacaaa ccagagcagc atgatggggc cgaagatgct cccgatcgtt
    66181 ccggagcccc ggcgttggat catgaacaac aaggcgacca gcgcgacggt cagaggcagg
    66241 atgtaggggg taaacacatc ggtggtcagc gtgagccctt cgaccgcgct aaggaccgaa
    66301 atggccggtg taatgatgcc gtcgctatag agaaacgcca cgccgatcaa accgagtgtg
    66361 acgacaacgt tgagcccttt gcggagatcg accgggcggg actgctggct gagcgccagg
    66421 agtgccagca tgcctccttc gccctcgttg tcggctttca tgacgaacag cagatatttc
    66481 acggtgacga cgagcacgag cgaccacacg atcatggaca gaatccccag cacgttgtcg
    66541 ggagtgaccg gcaggccatg cgattcgtga aaacactcgc gcagggcata cagcgggctg
    66601 gttccgatgt cgccatagac cactcccatg gcggccacgg tgagggagac ggaagaagat
    66661 tggttgttga gatgcttcgg catggatacg tgacaggggc ccgacccgat tcgcgatggt
    66721 cctgtgttgt gacgggtcga actataccat gttggatgag aggatgccgg ttggttccgc
    66781 agggcgtcgt aagccgccgc atctgctccc atgtggagag aggtgagtgg ccgcgcatcc
    66841 ggcctggaag ttgcgcgaat cttctgctag attccccgtc ggcccgtgcg aacgatcata
    66901 ggagtcgcgc cggccaagtc ttcgaattca tctcacaaga aagccgctga gaccgggagg
    66961 gtcgcctccg gccggatctc gctttcggaa agcccaatac gcacctatga cgtctccaac
    67021 gacaccgacc atttcatccg cagcccagca acgcatcgtc gatctcatcg ccaaggaact
    67081 tggagtgacc gccccgcaga ttgcggccgc ggtgacgttg ctggacggtg gagcgacggt
    67141 gccctttata gcgcgttatc gcaaagaggc caccaacaac ttggacgaca cgcacctgcg
    67201 caccctggaa gagcgcctct tgtatctccg tgaattggaa gagcggcggc agacgattct
    67261 cgcgtcgatc gacgagcagg ggaaattgac cgacgagctg cgccgggcgg tcgagcaggc
    67321 tgcgacgaaa caagccgtgg aagatatcta tctgccctat aaacccaaac gtcgcaccaa
    67381 ggcgcagatt gcgcgcgagg ccggactgga gccattggcg aacgcgctct tcgccgaccc
    67441 gacgctcgat ccggaccagg aagccgccaa gtatgtcaag gtggtagctg cagcggaagg
    67501 cgtcgaagcc gtcaacgtac ccgacgcgaa ggcggcgctc gagggcgccc gcgatattct
    67561 ggtcgaacga ttcgctgaaa ccgctgagct gctcgcgacg ctacgcacaa aattgtggaa
    67621 cgaaggcatt gtgacctcta cggtcatgcc cggcaaggaa acggcggaag aagaaaagtt
    67681 tcgcgattac tacgcctatt cagaaaccat ccgtaccatt ccctcgcatc gtgccctggc
    67741 gatgtttcgc ggccgcacgt tgggtgtgtt gaagctcgac ctcgggttag gggaaagtct
    67801 cgaagccatc gtgccgcatc cctgcgcggc gatgattgcc gcccacttcg gtattgagaa
    67861 tcgcgggcgc cgtgccgaca aatggctgac cgatgtctgc tattgggcct ggcgcgtgaa
    67921 agtgcatctg catctgagca ccgagctgtt gctgcaggtg cgcgaagccg ccgaagcgga
    67981 ggcaatcaag atcttcggtc gtaatctcca tgagctgctg ctggcggcgc ccgccggtcc
    68041 gaaggccgtg ctcggtctcg accctggtct ccgcaccggc tgtaaagtgg cggttgtgga
    68101 tgcgaccggg aaattgctgg acacggcgac gatctacccg catcaaccgc gtaacgattg
    68161 gcagggggcc ttggcgacga tcacccagtt ggtgcttcgg cacggtgtcg aattgatttc
    68221 catcggcaat ggtaccgcaa gccgggagac cgacaaattt gccgccgaag tcgtcaagct
    68281 ggtggcggag cagaaaccgg aacagaaact ggcgaagatt gtggtaagtg aagccggcgc
    68341 ctccgtctat tccgcctcgg cctttgccgc cgcggagttt ccatcgctgg atgtgagtct
    68401 acgcggcgcc gtatcgattg ccagacggtt gcaggatccg ttggccgaac tggtcaagat
    68461 cgatcccaaa tcaatcggcg tgggccagta ccaacacgat gtcaatcagc gggccctggc
    68521 ccgatcgctc gatgcgacgg ttgaagactg cgtgaatgcg gtgggcgtgg acgtgaacac
    68581 cgcgtcggct ccgttgctgg cgagggtatc cggcttgaat cgcgtgctgg cgcaaaacct
    68641 cgtggagtac cgtgacaccc acggtcggtt ccagaaccgg cacatgatcc tcaaggtacc
    68701 gcgcctcggc gaaaaaacat tcgagcaggc ggcggggttc ctgcggatca acgacggcga
    68761 caatccgctg gatcgttccg ccgtccaccc ggaagcctac ccggtggtcg agcgcatgct
    68821 ggcgcggctg aataaaggca ttgccgaagt catgggcaag ccggctgcat tgaaagaact
    68881 ttcgccggcg gaattcaccg acgagacctt cggtctgccg acggtgcgcg atatcctcac
    68941 ggagctggaa aaaccaggcc gtgatccgcg tccggagttc aagacggcca ctttccggga
    69001 cggcgtcgag tcgcttgccg atctgcaagc cgggatggtg ctcgaaggag tcgtgaccaa
    69061 cgtcgcggcc ttcggcgcat tcgtcgatat cggcgtgcat caggacggcc tcgtgcatgt
    69121 gtctgcgctg gcgaacaagt tcgtcaaaga tccgcatgaa gtcgtgaaac cgggccagat
    69181 cgtgaaggtg aaggtgctgg ccgtcgatgt gccgcgacag cggatttcgc tgaccatgcg
    69241 tatggaggat gccgcgacgc cgcccttaca gcctgatccc cgtgtcggag ggccgcgtga
    69301 cacgcgcgac cgacgccccg ccgatcagca gcggggggga tcccgtgagc ctcagcccat
    69361 cggggcattt gcactggccc tggcccgcgc caaggagaaa aaatagcgag ccgcctgctc
    69421 gcctccggcg cgctggtgag tggctcaggg agtgaccttg aagaaattcc cgcctttggc
    69481 ctgctcgtcg tcgctgcccc aggcaatctg cttatccagt cgcttgagga gcggcacatg
    69541 cttggaacag tggatgtagg cttcttccac gccgatgagg acccacgcct tggggtggcg
    69601 cccgcccttg atgtgagcgg cgtcggcgat gcgcgccgga agctgggaga ggctctccgt
    69661 ttcgctcggg ttgagtacac gcgcagttcc attgacgtgg agcccgaccg tgctctgata
    69721 gaagtcggga aaaatcaacc cgatgtgcgg attctccaga atgttcccca gactcgccag
    69781 caccccgttc ccccggtatt ccgggtagac cagcgtcttc tcgtcgagca cctgcacgaa
    69841 tcccggttcg ccggctcgaa aggaacaatc acacgcaccc ttcccgtcgg cggtggcaat
    69901 gaagaccatg tccatccgtg cgatgaactg ttgcatctgc ggattcaggt ggggcagggt
    69961 ttggtgggca taaaatgctg ccgcgcgagc cgaggtgccg aatcgctctt gtgcgcgctg
    70021 ttcgcctgat gatcccggcc tggtcatatc gaacgctcct tgtggaagat gtctaagacg
    70081 gtgctgcaga ggtgattgtc cctcatgcac gctggcgctg tcgatgagtg ttgtcgtgga
    70141 gaagtgtaca aagacccgct tagcccccgc cttcgtactt cttcagtttt gccaacagcg
    70201 ttttgtagtc gacccggagg gtttgggcgg ctttggtttt gttgccgccg ctggattcca
    70261 gcaccagttg aatatgcagg cgttcgatct cgtcgagggg caggtgcgac cggtcgtcgg
    70321 gtgatgatgc cgatgccgcc cgagggagca cctgcaggac ttcttcgctg gtgatcggtc
    70381 cggagttcgg actcatcagg acaatgcgtt cgatcacatt gcgaagttcg cgcacgtttc
    70441 caggccagcg gtaggtcgcc aggtgtgcga gggcttccgg gctgagcgtt ctcgttgcgg
    70501 tgcccgggat gcgaaggtgg tgcagaatat gatcggccag cacggcaaga tcctccggtc
    70561 gttcgcgcaa cggcggcaca tgcagcgtca ccgtgttgat acgatacagg agatcgtctc
    70621 gaaaccggcc ttgcagcacg agttcctgga tgtcccgatt cgtggcgcag atgatccgga
    70681 catcggcccg gagtgtgcgc gtactgccga caggacggta ttccttccgg tcgagaaagc
    70741 gcagcaggct gacctgcatc ggtcccggca tttcgccgat ctcgtccaga aacaacgagc
    70801 cgccttcggc ggcggcgatc agtccgggtt tcgcctgggc ggcgccggta aacgcccctt
    70861 tctcgtaacc gaataactcg ctctccagca actctcgact caccgctccg caattgacgg
    70921 cgataaaggg cccgccggcc cgacggctgt gcgcgtgcag cagccgcgcg accacgtctt
    70981 tcccggaacc ggtttccccc tgaatcagca ccggggcctg cgacggcgcc acttgcgcga
    71041 tctgtgtctt caccgtccgc caggcctggc tggtcccctc gacgatcgcg tcctggcgat
    71101 acccgccgtg ccgcgccgcc agattctcgc ggcgaagctc ccgcaccgtg cgaagtcttg
    71161 cgagagcagc cttcacggcc gcgccgtcga acggggtgtc cttgatgagg aaatcccacg
    71221 cgccgtcttt catcgcagcc acggcgtctt tgacgtcgcc atggccggtc aggacaagca
    71281 catcgacgtc cggttgatgc tccttcaccc agcgcaagat cgcacggccg tcgagaccgg
    71341 gcatgcggag atcggtgatg acgcagtcga aggaggccgt acggagtcga tcgaggcctt
    71401 cctgcccgcc gcccgccgtc tgcgtgtcgc agccttcctc tcggagcgcc tgttccacga
    71461 cgagacggac aaattcttcg tcgtcgacaa tgagggcgcg catgtgttcc atggtcatga
    71521 ggtcacgctg aagcaggatg aggtgaagag gatggccggt cgacgacgga ttcggcgcgc
    71581 tccggaaagg ccagatagaa tttactgccc gctccgggag cggattccac ccagacccgg
    71641 ccgtcatgtt tgtccatgac gagtttcacg atcgcgaggc ccacgcccgt gccctcgtat
    71701 tcctgtgggc tgtggagccg ctcgaagagc ccgaagatct tgtcgtagtg agacgggtcg
    71761 aagccgatcc cgttatcctg aacccacagg atgcgttcgt gttccatccg ggtgccgccg
    71821 atggtgatgg tcggcggtga ggcgtggcgg gagaacttcg ctgcattgtc gaggaggttc
    71881 gcgatcgctt gacggatgct caccggttca ccgtagagat cggcaaacgg caaggcgacc
    71941 tggattttcg gcttcgggcc ctggagtccg ctgaaccggt cggtgatgag ggtggtgatc
    72001 atttccaaca cattgaagcg ctgacgaggc aggtcctgtt gctccagccg ggaatatttg
    72061 agcagcgcgt cgatcatatg ggtgaggcgc agcgcggagc ggcgaatcac atcgatctgg
    72121 tgccgggtct gcgcgtcgcc gccgtcggcg aattgttttt ccagcaggga ggagaagccc
    72181 tcgatttccc gcaacgggcc tttgaggtcg tgcgcgacgg aataggtaaa cgcttcaagt
    72241 tctttggtct tccgggcggt cgcggcggtc tgttcgcgca ggctggtgac catggatgag
    72301 agggaggcgg ccaaggtgcc gacttcgtca cggccgggcc aggattcgaa ttgcgcggtc
    72361 aggtcgtggt gagcgacacg gtcggccgtg acggagagac gttggagcgg cttggcgatc
    72421 tgccgattga cggagacata gatgacggtg atcaccagac cgagcacggc gaccaggctt
    72481 aacgcaatca gccgcgcctg ttggagcaac agatcgcctt ccactttcat ttccacatcg
    72541 atatcccgat gggcggcgac cagttggtcg agattggtca tcagttccac gatgaggccg
    72601 tcggtcttcg ttaccggtga gggtgccacg ccggcgcgct gaggtccggt gagcgcggtc
    72661 tgccacaagg cggacagctc ctggaggatc agatcgatcc gggcgatggc gcgatcttcc
    72721 tggtcggcga gatccatgcg ttgatgctgg gtcagcatgc ccaggagaat cgggtgggtg
    72781 cgggccgcat gggtggaacg atacaggtcg agggaactgc ggatctgttc ggcgtggcgg
    72841 ctgagccggg caagggtgtc ggccgcatcg gccttcgtga tctgctccag aaacagatgc
    72901 agcgtgaggt tcatttcata ggctgaacgt tgcatggtgg ccgcggtcac gatggcgggg
    72961 accgtaatgc gtttgtggcg gtccacatag ctgttgatgc gactgaggta cacgagcagc
    73021 gctgtgaggc tcaaggccag caggctgagg atgactccga aggagatgaa gaatttttgt
    73081 ccgatcgagc ggtcgtggag ccagcgcatg caggcctcgt gtgagggcct acggtatcac
    73141 agtgactggt gtggtgccag taaggttcat gtggaatggg cggtcacgga cggcatcgtc
    73201 ttccgttcag gttcgccaat gaaccgttgt ttgaatgact ggcgctacgc gttccgaata
    73261 attggataat ccgaggctat cgtgcttcct ccacggcttt cgtccaggct ccttgcggac
    73321 tgaacagtgc ggcgaagacc cgatcccatc cgccgagatc ttcgatcgta aatacatgcg
    73381 gtggatggac gaaggcagat gcagaggccc tggcgacagc ctcgttcgcc ggccggaaac
    73441 cgaattccac aaaggcctgt tgggcttctt ccccgttgag gaacgagacg aaggcatcgg
    73501 ccagatcccg cacatggtgc cggtcggcgt ttcgatcgat caccgcgata gggttttcga
    73561 ccacgactgt ttcggccgga aagatcaact cgtaaggacg gccttgtttg atccgcggca
    73621 gtaattcgtt ttcataggtc acgatgacat cgcccacgcc gcgctcgaat gtcgtgaccg
    73681 attcacgtcc gctcttgtcc atgaccttga cgttacgttg cacgcgcctg acaagatcgg
    73741 cggcatgctg atccaccgcc gcgccggcga gccctttttg ttttccctgg ttgaggcccg
    73801 ccccgtagac cgtaatgacg tcccacatcg ccccgccgga tgttttcggg ctcggataga
    73861 ggacttcaac gcctggcttg gtgagatctt cccagccttg gatggatttg gggtttcctt
    73921 tgcggacccc tagtgcgacg atcgatgtcg tcacgatgcc tttttgcgct ccttgcttcc
    73981 agtcgtacgt cacgaggcct gccttcacca actgctgcag atcgctctcc aacgacaggg
    74041 cggcgatatc cgcctcgagc cctccgacga cggctcgtgc ttgcgcaccg gatgcagcga
    74101 atgaactccg gacctgaata gtttcccctg tcttctgctt ccactgccgg atgaaggcgg
    74161 gaatgattcg cttttcgtag gcctctttcg gcacgctgta ggccgcgagc aggagctctc
    74221 gggtctcggc gccggcgatc gccggtagga ttaccaacag tgatacaagt gccgtgaggc
    74281 cgttgagggt cttgatgttc atgttgatac ccactcctgt gatccgtaca aaatcggttg
    74341 ctgcctattt cctgtcgagc cagcgcatca ggtcgagcgg ccggggaccg gtcgcccgga
    74401 tttgacgccc ccgtttctgg aaaaagacgg gaaggccacg acgtgtcgct tcgatccgga
    74461 cctttctcgc caacgtatgg ttcacgcgat cgaggatcac tacgacggcc tccgtatctt
    74521 tgcgaatgga cctcgtctga tccctggttt tgcgcccgct ccaatgttcg acgcatccat
    74581 ggccgccggc acgtgcccgc tgtgtgattg catccaccga atcgccgccg acgaccagga
    74641 ttgccatggg gtttcctttc tctcgtgccg gatggcacta gggttgtgaa tgcttctcgc
    74701 ttccgcgttc cagccctggc tgtacaggca tggacgacag cgcctctcga tagcggatct
    74761 ggagctcttc ccgttgatcg ggggcgaaga ggcggctgcc tgaactgacc ttgaccagaa
    74821 attcctccgg ctcgaatgtc acatcgtgcg cggcagatgc ttccggaata ttcggcagtt
    74881 gccgatcgta tttgctgata tcgggaccgg ggctgtgccg gtcatgtacc ccgctgatgg
    74941 tgaggtgcag gatgggacct ctgatggaga tccagccggt cgtcgtttct tcatccccct
    75001 ggtccgtggc ctggctcagg tggaagtaca ccctctccgt cgggccggcc tgtgcgaggc
    75061 ccttcgcgag aggcggcgcc aagacggtaa tttcttcgtt ccggaaggcc ctcgtgcgat
    75121 ccgcttcccc gacaaacaac cgatgaatga cgtttcgccg ctcccatgcg cggaccccac
    75181 ggagcagggt tccgacctcg gttgccgtca ctgcggcggg atgcgcattg gccgtcgagt
    75241 ccggatcctg ttccactcgc acctcgctca gacccgactg atagatggtc ttcggcggcg
    75301 tgaccggtct gccggcgcaa ccggtgacca aggtcagtcc gatgagggcg agcatcaccc
    75361 gtttcgacaa aggatgtgtc tgctgtgctg tgttccggat gccgttcatg gtttcacctc
    75421 ctgccgctga acgagaccga cgtctcccgt gacacgatcg aatccgtatt cctgaaaaag
    75481 ggcttgggtc tggggcgcga gcaagaactc gatgaagtcg cctgcgccgg aaacgttctt
    75541 cgccgtccat acggctgcga cgccgtagcg gaccggcgtg tgggattcga ccggggccgt
    75601 atcaaggata cggaccttgt cgttagaggc ggcatcggtg cgatacacca cgccgacttc
    75661 ggcttcgccc ttggccacga gatcgagcac cgcgcgcgag tgctcgccga agacgtattg
    75721 ggacttcagt ttgggttcga gtttgctgta cttcaaaaac tgagcggcga cttttcccac
    75781 cgatgaggtc ttgggatcgc cgatcgcaat tcgccggacg ggcacggttt cgaggtcatg
    75841 gatcgtccgc accggagccg gaagcgcggc cccggtgatc aggaccagcg aggtgccggc
    75901 gaaggcgcgt ttggtgcctt gaatgatcag ccccttcgtt tcgagctgat cgatttcgtc
    75961 aaagagcgac ggtaggaaaa tgtcgaccgg cgcgccttct tcgatctgtt tgcggaggct
    76021 ttgtgagggg ccgtagatga cccgcacgtt gatggcaggg tgctcggcct caaatcgtgg
    76081 cagcactttt ctgagcgcgt cttttaagct gttggcggcg gcaatggtca atgtctccgg
    76141 ctgggcctgc gccgacggca ccactccggt gatgccgatc agcatactga agaagagcca
    76201 gatgccgaag aatgggtttc gtcgtgtcat gatcgtcctc ccgtgactgt cgcgtgttca
    76261 ggttgtctag aaaaagaact gatattggac attgattcga ttctcgatga ggtctcgtcc
    76321 gaatcccggc tggctctcga acgggaaccg gtccggggcg cggccaccgc tccccagggt
    76381 gatgttggag taatcgacga cgagccggtt gttgtaagta ccgtcgaagc tgtaattgat
    76441 cgcggcgccg aactccttca ccagatcgtt gacctgctgc gtcgagggat ccataaaccc
    76501 gtagcgaacg gccagttcaa gcttccgtgg gatgatgtat ttacccacct gggcgtacca
    76561 accgtaggcc tggccgagat tgacggccgg gcctaatacg gttgtggtga ccggatcgaa
    76621 cgggattgtt ccggaatcgg cgttccggac ccgttggtgg cgatagtatc cttcggcctg
    76681 gaaggaccag ccctgatact tcgcgataaa atcggcttca taggtttgaa aatcccagat
    76741 gccaccgccg agcagacgtc cgttgtacgc tttcgtgaca cgttgacggt aggcgcgatc
    76801 ggtaatatcc gagcggatcg aactcaggta gttttgggca gggttgtaag catagccgaa
    76861 ggcgatggcc atctgcggcg tgcgtgaatt cagaatatct ccctggccat acccgggatt
    76921 gccggaaatt ttgtacagca gacggaccgt atacatcatc tcgcccgtca gaatgcgggt
    76981 gtcgtaattg aatgtacggc gctgggcggt cggaaggctt gaattgttgg gaagcgcttg
    77041 gctgacggcc gtgccttctc gtgccaggtt ggcaccgact cccccccaga ttccgaaggc
    77101 atagttgaat ttatactggt cttcatcgct ggaaatgctg acgccgatat cgcggcttcc
    77161 ctgttgattg gctgcaaacg cgttctggac aatcagattg tccgcgaatg ttgaggttgc
    77221 catggaactg atcgtggcgc gattgaacca gacgcgctgc tgacctgcct ggatggttaa
    77281 ccaggggatg tgccaggacg agatgtaggc gtcgaggagg cccgcacgtc cgctgcctcc
    77341 ctcttgatcc catgtcgtct gatcgaaggc ccaggaaatg ttgtagcgaa agtcaggatc
    77401 gaaggcgtag cccaggaact gaaggcgggc gcgcggcacg ctgaactggt tggagtcgct
    77461 ttgcttactg acggctcggt attccacctg accgccgagg atctcgggat ttctcgaatc
    77521 gccgacggtg ccccatcccc cattgtaggc actgtggctg taccgaacct gggtgagcaa
    77581 gcgcagtttc aaaaagaatt tcgaccccac gcggaagttc agcccgttgt tgacgtcgat
    77641 gctgtagttg ggtttggcaa tctgttcccg ggtctccagg actctcaggc ccttatcgta
    77701 ttcttcctgg gtgatgatgc cttttaagaa aaggtatttg aggcccgggt cttcgcctga
    77761 atagtactcc cacttcccat ccttcttaac cagctggcct tcctcgaccg cttgaacgga
    77821 ggtgatggtt ccagcgatca agctgccggc caccacggca attccaaaaa aagtgtaggc
    77881 tctcatctga tctaacctcc gagtgggtga tattcagaac cgttgatatc ttgtaaaatg
    77941 ttaattaata tagaatttat taaatatcta tattccggtg agaaattaaa tacatcaagt
    78001 tatgtaagat tgtcaacaat tctgattaaa atttatgtgc ccatgaaata cttctccttg
    78061 ctggaatcaa tacttgacgt aggtaaagca ttatgtcatt tttcgtcccg tcatgaagaa
    78121 ggcgcccgct gctgaacagt ccattcaggt agagaatctc ttggccccga cacgccaagc
    78181 caaaggcttg tcacgcatcg agcttgcctc gcaagccggt attacacggc aagccgtcca
    78241 tgccatcgaa acgaatcaat accttcccac tacagccgta gcgctccgcc ttgcggatgc
    78301 tctgggctgc cgtgtggaag atctcttccg gctgcttcct caagaagaca tcgttgaggg
    78361 gcaatggtcg gactcggcaa cggttcagaa gacgccgcag gaagccgttc gcgttaaggt
    78421 cgctcgggtg ggcgcgcgat acattgtccg acccgtcgtc gatctgggag aggtgctcaa
    78481 ttatgcggtc cccgcagacg gccttctggt tccctcccgt ggggtccgga caggagccgc
    78541 cgcttcgccg cgtcgcgtgc aggtccgcct gcttcgggac cgccgtgtca ttgagcagga
    78601 gatagccgtg gctggttgtg acccctcggt ctttctggcc ggtgattatc tccgtcggca
    78661 tcaggacagt tgcactgtgg tgggatggaa tctcggcagt accgcggcaa ttgaagcgct
    78721 caagcgggga gaggtgcaca ttgcgggcgt gcacgttgtg gatgcgcagt ccggagagtc
    78781 taacttgccc tatctgcgcc gccacctgaa agggaatgac tatctcgtgg tcacctttgc
    78841 ggtgtgggaa gaggggctgc tggcagctgc cgggaatccc aaatcggtgc gtggcgttga
    78901 ggatctggta cgaccggaga ttcgattcat caatcgtgaa gccggcgccg gtgcccggct
    78961 tctcctcgat cagaagctca ttgcggccgg tatgactccc gagagtgtcg ccgggtacgc
    79021 aaccatcgcc cgctcccatt ttcaggtggc gcgatcgatt gccgaaggac atgccgatgt
    79081 ggggctcggc gttcgcgcgg cggcgcagct gttcggcctg gaatttcttc cgctgcaaca
    79141 ggcccggtac gatctcgtga ttcctcgcgt gcacatcaac gaccatccga gtatcgagag
    79201 ctttctcaac attctcgtca gtcgcccgtt tcgaacggag atcgatgcgc tcggcgggta
    79261 tgacatgagt caaaccggga cggtccacgc attacggtca tgaaacgtca gtaccgctca
    79321 caaccactgt tgttatgtgc tgccttcctt atggggctgc tgagtcttac ccccgcaccc
    79381 tgtgtgtctg ccgagcctct ggtcatcgcc gcttcaccga gtcttgccac accgcttgag
    79441 gcgctggcgc gggcttatga agccgcccat ccagatgtac aggtgaagct gtattttgat
    79501 tccgggttgg atcttcgccg gatgatcgcc ggtatggaga atagcccgac ggggcagtat
    79561 ttcatcggca cggggccgat ccacctcatt gcaccgggcg gcgacgaact cattacccgc
    79621 ctccagcaaa aatattatgt cttgcccggt tccaaacgac cctatgccac cgtctctctg
    79681 gtgatgattg ttcctgaatc gctggtcgag gcgcccgtgt ctttcgtggc catggcccgc
    79741 gactcgcgta tgcgcgtggc cgtggcggat ccggatatga cgctgttggg ccaaaagacc
    79801 cgtgacctgt tcaaggcatt ggggattgcc gacgtcctga acgaccggat ggatgtggcg
    79861 acggatgccc gcggggtggt ggaccatgta ctgaacgggc aggccgacgt ggggattatt
    79921 ttcggcccgg acgcagtgga aaaggccgat cgagttcgag tggtcgctgt ggcatcggag
    79981 aaggaggggc gtccgattct tcattcgatg gcgatggagc gatattgtcc gaaccgtacg
    80041 ctgtgcgacg attttcttgt ctatgtgcag tcggctgagg ccggcactat tttgaagcgg
    80101 ctcgggtatg gcgtgccgac gaagccgtag cgcgtgctca atgtgagagt cagtagttgg
    80161 aggatggtca tgcgggtatc aagtgaacat acagggttgc tgaatctgtg catggtggtg
    80221 ttgggcattc tggtgctggg aggatgtgcc aggctgccgt ataccaaaac ggttgtgcat
    80281 gaagatcaac gggtggccgt gaggcttcag gccgaagtct cgtcggccgg atattcgcat
    80341 ccggtcgaga tccctccgcc atatatcgcg gccattcttg gcggattgtc tttgcgggag
    80401 cagcagcgcc tgccgcttcg ctggtttgcc gaggaggtgc ccccacgccg ggtcttccgt
    80461 gaggatgagg tagaagtctt ggcgcctcac cttgcggcgg cgctgcaaca agtggggccg
    80521 gaagagcgtg tctacttcga ggtgttcgcg cctggcgtga acccccggta ccgtcgcgac
    80581 gtcaccggag ggtggctggc cgttcgtgag caatatctgc atatcacgat ggactacttc
    80641 catacgcaac aaccggtcga aaagtcagac gcctacgact acaactatcc cactccctgg
    80701 acttctccca atacctacct cctctacttc gagccgggcc gcttttatct caccgacccc
    80761 aagaccaatt cgcgcgcggt ggactttcgg gagtttctca aaacgctccc gccaccgtag
    80821 gccaccgggg ccgattcaat tcagcggaac cctgttccat catcacatca tagtgtgggc
    80881 gagagcctgt gcggaggacc tgagatgcaa aggatggcgt cacccgtgtc actactcatt
    80941 cgatcgtatt ccagcttcgg cctatccggt gtgggcttgg ggctcctgct gtgctgcctg
    81001 agtctgttcc attcgaccga cgcgatctcg gccgaggccg gtcggttggt ggagaaagac
    81061 gggcgatatg tgttcgtgga gagtatggat ccggcgatga agctgctgtt ggagcgggct
    81121 gtgaagcagg gcacgatcac ccaggaggaa tatgacgggg tggtgcgggc ttcacaggag
    81181 cgtgcctatc tccttcagcc gagttttcgg gcctggtacg atcgcggatt caatttatcg
    81241 atgaacgaca acgcgttttt tctcaagatc cgtgcgaggt ttgcgactcg ctacacgcaa
    81301 cgataccgga atgacgccta ccgcgaatcc ggggactcca agaattttcc cgaattgctg
    81361 ggcgtcttcg gagattaccg ggccaaccga tccatcgacc agtcctccac gttcaatttg
    81421 cggattgcgc gtctctattt tatggggcac ctgttcagcc cggatttcag atactacatt
    81481 caattggcgg gggaaacggc ggaaaatgcc caggcgccgg gtgccttgtc cgtgctggat
    81541 atgaatgtca cgagcaccca tctcccgtgg atgaacgtcc aagttggtca gtataaagtc
    81601 tatttcaacc ggtcgcagat caactcgacc gcctccatgc agttcgcgag tcgtgccttg
    81661 gtcatggatg cctttaccgc gagcggcatc aatcgccggg atgtgggact cacgatcatg
    81721 aacgatgaag aggtctaccc gatcaactac tacctgggag tctttaatgg tgtgggcccc
    81781 ctggtcaacc ggttcgctca attcaacagc gaagaggcga cggtggggtg tccgggcggc
    81841 gccaccggag gcaatccctt tccgtcaccg gcagggtgtc cgacgaatac gcgtaacttg
    81901 aacgccaatc tccggaccaa tgtgaatgag ttgatgtacg tggcgcgact ccaggcgaat
    81961 ctcatgggcc ggccgggata cggtgagggc gatctcgcct attcggagag tccgcaaatg
    82021 gcggtgggcg gcgggtatgc ctataatccc tcgatcgata cgagcagcaa caacgccttc
    82081 gtgggaatcg atctggcgaa tttgcacgtg cggcggtcct tggcggcgct gggcaatgga
    82141 cgaatgctcg gtcagggggt ggtggatttt tcgacctgga ccctggacta tgtgttcaaa
    82201 tatcgcggct tttctctgca aggggagtat tggtatagaa atgtgactcg ccacaacaag
    82261 gggctgccct gcatgcaaac cgccgtggtg ggagggcctt gtacggtttt cgcgccgcag
    82321 gagttcggca attcgaccgg ctggaccgtg cagtccggct actacctgat tccgagaaaa
    82381 gtggaagtcg ccgcgcggta cgcctggtgg gatcctgata ctcgctcagg cggcgatctg
    82441 atcaagcaag tggatgtctc cctgaactgg tttctcagcg ggacctatga ccaccaaatc
    82501 atggtgacct attccaacat cgcgatgggc caggacgggt tcgccatcgg gcggagcgct
    82561 cctttgccgg cggtgggcgc gaccttccct tccgggacgg tgccgctcga ttcgcgagcc
    82621 ggaacgttga ttgaaaatgc cgtccgcatc cagtatcaaa tctttttcta aggagcctcg
    82681 tctatggcac gtctgctgct gtgtgtgatg ttcgtgctgt tgtgtcggcc cgccatcgca
    82741 ttggccgatg acatcgcggt ggccgccgcg tccgacttac agttcgccat caaggaattg
    82801 atcgtggagt atgagaagca aaccggtcat catgtgaaac tgtcgctggg gtcgtccggg
    82861 aatttttacg cgcagcttca gcagggtgcg ccgttcgacc tctacttttc agcggacatc
    82921 gggtatccga agaaactcga ggaggccggt ctgacggtgc cggggtcgct ctaccggtat
    82981 gccgtgggcc gagttgtgtt gtgggcgccg aagcagtcgc cggtcgaggt gtcgaagggg
    83041 ttgacggtgc ttcgagacgc ggcggtgaga aagattgcga ttgccaatcc caagcatgcg
    83101 ccctatggtc gtgcggcggt agcggccatg gagcagagcc aggtgtatgc agaggtgaag
    83161 gatcgcctgg tgctgggaga aaacatttcc caggcggcgc agttcatcga gtccggggcc
    83221 tgcgatgtcg gcatcattgc cctgtctctg gcgatggcgc cggtcatgaa gagcgccggc
    83281 tcgtactggc tgattccggc tgaggcccat ccgccgttgg agcagggcgc ggtcatcatg
    83341 aaacagtcga aacagcagga cgcggccagg cactttcttc agtttatgca aagccctcaa
    83401 gggcaggagg tcatgactcg ttatggattc acgctgccga attagtcgcg ggatgcctgc
    83461 gcttgcattg ggcgcagccg tgatgatcgc cggttgctcg gaggccgtgt tgttgaccca
    83521 tgaaaccggc cagggcggtg tagtgaccta tctttataaa gatgagcgag gtggtccgat
    83581 gggatcgccg taccggcgag aagccttgaa ggtgatggcg gagaaatgcc ctgccgggta
    83641 ccacgtggtg aaagacgggc aggtgcaaag ttacagcagc gggtcgagtg tggagggggc
    83701 tgagggcgag gaagggacac ggcggtgggg gattcagttc gaatgtaagt caggcgggga
    83761 cgataggact acgggcagga aatagagtgt cgcgatggat catcctgcgg tgaatgagtg
    83821 cactcataac ggctgacatg cctcggtcga aacggactag ggggacgcta tgaagctcag
    83881 cgcgagaaat caatttcaag gccaggtcgt tcgaatcacc gagggccagg caatggcaga
    83941 agtggtggtg aaggtcggta acttggaagt ggtgtcggcg attaccgaag gctctgtcaa
    84001 aagtatgggg atcaaagtcg gcgatgccgt cacggttgcg gtgaaggcga ccgacgtcat
    84061 cgtcggaaaa taatcttctg tcgcctgtcc gtctgcggta gcatccggta ggagtagagc
    84121 cggtacaaca gtatcggtcg tgaacgagat catgaactgg attgcgattt gggtgacgtt
    84181 caagctggct gcttccacgg cgcttgtcct catgatcgtc ggcctcccga tcgcctattg
    84241 ggtgagcttt tcgcgccggc gctggaagtt tctcgtcgag tcggtggtgg cactcccatt
    84301 ggtgctgccg ccgacggtgc tgggatttta tattcttgtc gccatcgggc cgcacagccc
    84361 gctcggacgg ttttacaacg agctcatcgg gcatccgctg ccctttacct tcgaaggcct
    84421 tctgctcgct tccattctct atagcctccc gtttgccgtg cagccgttcg ctgcggcgtt
    84481 cgagcaggtg gatcggcggt tgatcgaagc ctcctggacg ctgggcgtgt cgaagctcgg
    84541 cacgttcttc aagctgatcg tgcccttgtc taaagccgga ttgctgaccg gtgccgtgct
    84601 gagcttcgcc catacgatgg gagagttcgg agtcgtcttg atggtcggag gcaatttgga
    84661 gggggagacg cgcactgtgt ccatcgatat ctacgatgag gtgcaggcct taaattatgc
    84721 cggggctgcc aagacggcgc tctttctcct ggtcgtctca tacgccattt tgctcctcgt
    84781 gtatgcggtc aatcgtaatg tgtgggcagc atggccgcgg aaatagatat tcaactcgtg
    84841 aagacgtttc ccggacgtgc gccgatcaag gcgcggatcc gatatccggt cgaggcttcg
    84901 actgtcttaa tcctcttcgg tccgtcaggc tccggaaaaa cgacgatcct tcgatcggtg
    84961 gccgggctgg aatggccgga agagggcacc attcaatttg tgtcgcgcac gtggctggat
    85021 accgcgtcag gggtcagggt agcgccgcaa gatcgtcaca tcggctacat ggcgcaggac
    85081 tatgcgctct tcccgaccta cacggtggcg ggaaacatcg ggtatggcct ccaccatctc
    85141 tccgcacagg atcgcaacaa gcgcgtcgat gagatggtgg agctgttcca gctgcgtgga
    85201 ctggagacgg cgaagccgcg ggaactgtcc ggcggacaac aacaacgggt ggcccttgcg
    85261 cgcgcggtgg cacctcgacc gttgttgctg ttgttggacg aaccgctttc cgccttggat
    85321 gcgccgacac gggtgcactt gcgtgatgaa ctgcggagtc tgctgaagca gctggcgctg
    85381 ccgtcgatca tcgtcacgca tgattggacc gaagccctca ccctcggcga tgtgatggcg
    85441 gtcatcagtg cagggaacgt gctgcaggta ggcccgccga tcgaggtgtt cagccggccc
    85501 cacaacgcgg atgtggcgcg tgtcgtgggg attgaaaccg ttgtgaaggg gcgggtggtc
    85561 ggatcctcgg acggtatggt gcaggtgagt gtgaacggga cccggttgac cgccgtggaa
    85621 ggtgaatcgg caggccccga tgtgttcgtg tgtatccggt cggaggatgt ggtgctggaa
    85681 cgtgggcaag ccaccgcgag cagcgcgcgg aatcatctgg ccgggaccgt catctcggcc
    85741 acggtgcttg gggcattggc gcaagtgacg atcgattgtg gatttccgct cgtcgccatg
    85801 gtcactcgtt ccaccgtcga agagttcggg ttgtcatccg gtgttccagt cgtggccgcg
    85861 atcaaggcgg gttcggtcca tctggtatcg aggcagagcg actagcagga tgttgaaaaa
    85921 gtccgtatct cgtcgggggc agagcgtatg gctcgtgacg tatagctgag aggagagacg
    85981 cccactctcg atttctggcc attcgatctt ttctgcgaac gagagacgaa ccacgcttca
    86041 ggaacgacga acgaagaaga ctaaaacgca ccggtgatac tggcgccgaa tttttcccgc
    86101 acgatgggcg tcgcttcctc gaggttcagt ttttttggga gtacgtgggc gactgaccgc
    86161 attttgtagc gtcgcggttg gtcggttttc ccctgttcgg tgaccaacac gtctgccacg
    86221 cagaggtaga gactgtcgtg gttctgttcc ggtggtgcca tccccggata accgcccctg
    86281 ccgctcatgg ccatccgcat catggcattg aggtccggcg ccgcgccgcc tcccattgcc
    86341 attccgcgta gggcttgcat attcaccccg ccgccccctg atccgccgaa cattccgccc
    86401 atggcgtcca tgtcgagccc gcctgcgttg gcgagcgggg tgcctccact tccgatcccc
    86461 gcaccgaatc cggccttggc gaccatttcc ggtctgaccc catctcctgc tttgtggcag
    86521 tacaccacct gggtctgtag ccagtaggca gcaccaaggg gatcgttcac gacctggtat
    86581 cccttggcca tcagcctcgc ggggatatct gcgggggtcg cgtgttgatt ttcagacgta
    86641 ttgcggattt gcacataaat ggtacgcgcg gtactgggcg gcaggaggat ggaggtgtcg
    86701 ttgactaatc cggagcgcaa gatgttgctg cagccggtga gcaacagcag cgcaacaagc
    86761 catccgcccg cgatacaagg agagaggtgt ctggtcactt gtctggtcat cgacggttgc
    86821 ttagaagtgg cctgccactg ccgcgctcaa tcgctgctgc accaagggag tcgcttcctg
    86881 ctcatcaagt ttcttctggt acacgctggc cgccaacctg gtttgataga caccggcagg
    86941 aggcgttgtc cccggcggtg aaaccggcag cgttccgccc gttcttgcca gctccgtaat
    87001 ctggagatcg gccacacagg cgtaattgat gttgtcattg gcatcgggcc gtgacggtcc
    87061 gccgaacata ttgcctacgg cattcccgag tccttctgct gcatcgaggc ccattccggc
    87121 tgcgcctcct atgagggcgc cctgcgggcc tgccatgccg gccatccccg tcagcccatg
    87181 gagccccgac atgatcgagc tgccgattcc tgatccatag ccgcctgcga ccaggtcttc
    87241 aaccggcagt tcgaccttag tttgattgca atagacgata ttggtcagca cgacataggc
    87301 tgccgcctgg ggattctgaa caacctcata ccctttgagg ttcagccgtg ctctcagatc
    87361 gtgtagcgac acagcttgat tgtctgacgc gttccgattt tggacaaaga cggttcgcgc
    87421 cgtcgatggc ggcagcatga ggctgttact ggccagtaag tcggtatccc ggacgttggc
    87481 tgcacatccg gtggagagga ggattcctgc caacaagctg cggaacaagc ctgcggtcat
    87541 ctgagctttc ccggacgtgc caccacacgt aggagtgagg ggtagtcgcg gcgcgattct
    87601 aggtgaacgg atcagaggtt tcaatcaaga aaatgaagat tccgtcaaaa aactctctcc
    87661 ccaaattctg tggatatctc tgttgatagt cacccggttg accgcgaata gacccgctaa
    87721 ttcatgcctc gcaccaggtt gcctatttat tgagcataga gaatgatggc ttccaggacc
    87781 acaatatcta gtagtgggcc tcttatgtgt atgtattagc attcatattt gtccaggact
    87841 ccaggagttt tgaaaatgag ctgtggatag tggctttatt tttttagacg atccatatgg
    87901 ctcttgactt atgcacagaa ggttttcgtt tctgatgaaa ttacaggggc tcggatcgca
    87961 aaaaccgcac tggaagaggc cgccagaata gtcaagaaag atgataggca gatgcttgac
    88021 agagactcaa tttatatgta accatcggtt acatgatgac atcaagcgag tttcgacaga
    88081 tccgtgaaca gttgggactt acgcaggagc agcttgccgc agcgctgcgc accacgcggg
    88141 tgtcggtcgc tcgttatgaa tccggcatgc gccggatcag cggcgccgtt caggtggcga
    88201 tcacgcaact ggccaacagg actcagattc ccatggcagg ggtcgttgct gccggtttgc
    88261 cgatcgagcc ggtgacccag tcggaatggg tcgaggtacc tcccggcatg gtgcgaaggg
    88321 gggagacctt tgcgctccgt gtgaaagggg agtcgatgat cgaggacggg attctcccgg
    88381 gcgacctggt cgtcgttcgc aagcaggcga cggctcacaa cggccagacg gtcgtggcgc
    88441 tcgtgaaccg ggaagcgacc atcaagacct atttcaagca cgcccggcac attgaactcc
    88501 atccggccaa cgctgccatg aaaccgtttg ttgtcacgcc tgaggatgaa ttttccatcg
    88561 aaggggtgct cgtcggtgtg attcgtcatt gcgagaacgc gtaattcaag gaggaataat
    88621 gatgatgagc catcagcaac aagccatggt ggagcgtgtg gacgaactgg agcgcatgat
    88681 tgtccgcctc aatggacgta tcggggaact acagcgagag ctgaagcata ccaaggggat
    88741 gacgttccag gggtggtccg gcggctcgcg cttgcgaact cttctcggcg gaatgaaacc
    88801 ggtcctgtcc gcctcacatc ccattcgaga gaacaggagg attgaacgcc atgatctctg
    88861 tctcttctga ccttgatgcg gtgacggctg gatgttgcgc tgattgcggg caactggtgt
    88921 cacggcgaac gccgtggttc gatgaaaagg cgaaggcggt gcgggaggtg tgcatccgtt
    88981 gtttggtgct cacctaccag cgccggcagt ttgaagctgg ttgttgcggg tgaggcggga
    89041 aggcatgctc gcggatccca ggtgatcgtc ggctgaatgg ccgacccgcc acagcttccg
    89101 gcgatatcct gtttccttcc gaaagcgtcg tgagtttctg ctgggaattg cttacgcgct
    89161 gcttcagtgc cctggcccgg ccgctgttgt tggcgagtcg actcctggct tggagtcgag
    89221 ccaatcagac cgttcggggg ctctggctgt cgtgctcgct gtctggacga ttgaatgggg
    89281 aacatgatcg catggttctc tccggtctat taatcgcgag gcaacattcc agaatcgttg
    89341 gttggcggcg tcaggctgtt ggtgtaggcg gcctgatgtt cccttaccgc ttcaagcctg
    89401 tagagtgctc cgtcagcaac gccatctttg gccggcactg atccctctcc tagcgggtta
    89461 ttctaagagt tgccccagca tgtgccccgc ttgtctgatt caataggtag gccattcccc
    89521 tgcgtcggga gacgtggtcg cgatcgtcac ttcttttctc agagggcacc ttcgtcagtg
    89581 tgccttcacc cggtagccgt tctcgttgcg ggcgtagatt aagtctggcc tcggtgaggc
    89641 ttgccacttc tgaggctact gactgggatt gcggctgaag cgcggaggct caggaaaagg
    89701 ttgaaatacg gaacgaccgg gattgcaact tgctcctgtc gatatcctgc gctgaggcac
    89761 cgaacttcga agttcgcgat gtcggttttc tattgcctgt gcgcttcatg atcacactgt
    89821 gcgccttccc aggatcgatg ccctccgggc tgcgatgcgc aggcgacgaa cggtgaggtc
    89881 ttgccagggc gtggctccgc tcgattcgct ctccacccgc agccgttcca tagatttgtt
    89941 gaggcggtgt cgtctggctc gccttagtac ggacgacttg cagtgcgcca tctagcggtc
    90001 gccatcggca tcccgcttca tcctctttcg tcaagttctt gtcgaattcg accgatattc
    90061 ctgcagtaag ggtgtgtcgt ggcctgggat ggatccgtgc gcgcacgata gtgatgacgg
    90121 tgtctgcgga atcatgaaga cgatggcaaa agaacccctg caagtattgg tattggtggg
    90181 ggcaatggtg atgatctccg ggtgcatgat caagccctat gtgttgaccg acgaggatgt
    90241 ccggacccgc gccaccaatg accttcagat ggtgtcgacg cttcgggagc cgctgtccgg
    90301 tccgatcgat ctctatgaag ccatcgcgcg tgccttaaaa ttcaatctgg acgccaaggt
    90361 gaaggccatg caggcgcagt tggcgcatca gcagctgaac gtggcgcatt acaccttgtt
    90421 gccgcagctg tcggcgaacg ccggatttga cggacgcaat tcctacagcg gaggcggcgc
    90481 gcaatcactc gttgatggtc ggcccgtcct cgagcccttt acgtcgtccg ataagaacgt
    90541 ggtgtcgggt aatctcgcgc tgagttggga cgtgctggat ttcggtcttt cctatgtgcg
    90601 cgcacagcag gcggccgata acgtcatgat tgccgaggag gagcggcggc ggattgcggt
    90661 acgcctcgtc caggatgtcc ggagcgccta ctggcgggcg gtcagcgcgg aacgggtgct
    90721 ctatcgggtg aaatttctgg atgagtccgt caacaaggcg ttggagcgcg cgcagcaaat
    90781 tgtcgataag aaattgcaga cgcccttgac cccgctcaac tatcgccgcg atttgctgaa
    90841 cattcagcgc gaggtgcagc gcctgtaccg tgagctgagc acggcacgag ttcagctggc
    90901 ttcaatgatg gggctgcctc cgggaaccga gtacgacctg gtcgtgcctc agcgggcgag
    90961 caccgttccg gtggtcgatc tggatactgg gcggatggag gaacaggcgt tgttgcttcg
    91021 tgccgagctt cgctccctcg actatcaaaa gcggatcaat gcgaaggaag cgcgggccgt
    91081 cttcctggaa atgtttccca acttgaagct gagtctcggc gggtattaca gcagcaacag
    91141 cttcttcctc tatcaaaact ggctggggta tgcctctcag ctcagttgga accttctgtc
    91201 ggcgtttcgc gtaccggcca aactcaaagc ggtggaggcg catcgacagg tgttggatgc
    91261 ccaaagtctc gcgctgacca tgacgatctt gaccgaggtg catgtcggcg cgctgcagtt
    91321 cgcccaggcg agccaggaat atctcaatgc caggagctac cacgagacgc aattggccat
    91381 taccgatcat accaagaacc tgtggctcac gaagagtacc aacgatctga ccttcattcg
    91441 ggagcgagtg aatgacgtgc tggctgaagt ccgcatggac agcgcgcagt ccggcgtcga
    91501 gtcggcctat gccacgttga tggcatcgat gggcgaagat gccgcgccga ccggcaccga
    91561 cgggcagagt gtggccgccc ttgccgatga actgaggaaa cagcgggtgc ctgccgtcga
    91621 cacggtgtcg gagtcaggaa ggaggctgga gtcccatgcg atcccattgg ctaattaatt
    91681 cgatggtcgc actgacgatc ttctgcgcgg cggtggcctt tccgcaggtc ggctggccga
    91741 aagacgaccg gcccgtccag ctgatgcgga gtgtcaacgg cgcgttgcgt ggcattgtga
    91801 aggccagcac gcaggccgtg ctctattccc agattcaggg gcggatcagc cagatcccgg
    91861 tgaaggaggg gcagcggttc gagaagggcg caacgctggt cgcaatcgac tgcgacaagt
    91921 atcgggcgga attagcgggg gccctggccg agcatgaggc caaagacaaa accttccaga
    91981 acaacaaaga actcaccaaa ctcaatgccg tcagcacgct tgaattggaa atttccgaag
    92041 ccgaggcgaa gaaggccgtc gcggccattc gcctggccga gatcaacgtg cgaagctgcc
    92101 atatcgcggc gccgttttcc ggccgcgtcg tggcggtcat ggtcaatgag catgaaaacg
    92161 tcttccccaa cgacaagctg ctcagtcttc tcgatgacac cagtctggag atcgagttag
    92221 tgctgccctc ctcgacctta gcctggctca agcggaagtc accgttcaca ttcaccatcg
    92281 acgagacgca tcgggagtat ccggcgaagg tgaaggaaat cggggcgaat gtcgatcccg
    92341 ccagtcagac ggtcaaggtg gtgggcagct ttggggcact cccggccgat gtgctcgccg
    92401 ggatgagcgg cacggcgcaa tttgcatcgg gacaaccgta accctatggc gacatccacc
    92461 caaccgagcg cacaggaact cgcgacgctg gtccatctgg cgaccctcac ccatttggag
    92521 gcacagatcc gtgtcgcgaa gaccgtccag gaattgcagt ttatctccgt caatgagacc
    92581 cgccggctgg ttccctatga acaggcctat ctgctgagtc atgccggtga agctgcgggc
    92641 gtctcccggg tgatctgtgc gtccagcgtg gccgtggtgg aacgcgatgc gcccatgatt
    92701 cgctggttgg aggagatggc acaggctctg aggcacgcac atccggggga tgacccggtg
    92761 accgtcacgg aggaaatgtg ccccgactcc tgccgcgagg gatggcgcga gcatgtcagg
    92821 ggccatgtcc tctggtgtcc gctgaaacac ccggatgaaa cggtgttggg tttctggtgg
    92881 ctcgaacggg actatccctg ggaggagaat gatcgggcga ttatgcaccg gcttgcgggg
    92941 agcttcgcct atgcctggaa ggccgtggcg acgcgccatc gaacctggtc cgtgaggatc
    93001 aaaacgccct cggtgtggtt gatggctgca gcggttgtcg gggcgctgtg tctgccggtt
    93061 cgcgtgtcga ccgtggcgcc ggtgaaagtg atggcgaagg agccgacgat tgtgagcgcg
    93121 ccgatggacg gagtgattgc cgaggtcctg gtgctgccga atacggtggt cacgcaacat
    93181 cggccgctgt tccgctacga agacacgaac ctccgcaatc agttccgggt cgcggagaag
    93241 caattggcgg tggcgcaggc cgaacatgcc caggcgattc aaggcggctt tgccgatccg
    93301 caacgaaaag ccgaggttcc gctcagagcc gccgaagcgg cgttaaagga gacagagctg
    93361 acctatgccc aggagatgct ggcgcaggtg gaagtccgtg ccccgcagga aggattgctg
    93421 ctgtacagcg acaaagccga ctgggtcggg aagccggtct ccgtcggcga acggatcatg
    93481 gatattgccg atccgcgtcg cattgagttg cgagtcgatc tccctgtgag tgacgccctc
    93541 ctgttacggg aaggcgccga tgtcgtagcg ttcttcgatg cactcccgct ggaatcgttc
    93601 ggggcgaccg tgagtcgcgc gagttaccat gcggaggtgt tgctcggaga tatgttggcg
    93661 tatcggatca cggctgaatt ggcgactgcc gatccccgta tccgcattgg ctggcaaggg
    93721 tcggcgaagg tgtatggaga acagggcccg ttggcattct tgttgttccg ccgtccgctc
    93781 atggccttgc gccaactggt cggagtgtag ccgatggccg gcctgtcctc agtgtccccg
    93841 ccccaagaac gcaccttgcc tccgctgaga aagaacctgc agttcctccg cggcgcgccg
    93901 acaccggaag gggtgccgac ctggacgatc gtcgatccgg ttcgcaacaa atatttccag
    93961 atcgagtggc aggtctatca gctcctccag cgctggtcct gtggcacggt ggaaaaactc
    94021 gtcgcggtgg tccggcgcga caccacctcc cggatccggg ctgaagacgt cgaagactta
    94081 gtgcggttct tgtatgccaa cagtctgacg gagcaatcgg ccagcgggcg ggtcaaggac
    94141 tacgtcgagc aggaagtggc acgccatcag gcctggtggc agtggctgtt gcatcactat
    94201 ctcttcatca aaattccgct gatacatccg catcgttttc tgcacgccac actgcccttg
    94261 gtggcgccgc tctacacacc catggcggcg tggctgttcg gaatgatcgg tgcgaccggg
    94321 ttgttcctgg tgggacagca atgggacacc tttttgtcga ccttccttca ttttttcact
    94381 tggagcgggg cgctgatgta tggcgcggtc ttctgcgccg tcaaggtggt gcacgaattg
    94441 gggcatgcgt atacggcgac gcgattcgga tgccgggtac ccacgatcgg tgtagccttg
    94501 atggtgatga tgccggtgct ctattcggac atcagcgacg cctatcggct gagttcgagg
    94561 cggaaacgtc tgtggatcgc cggagccggg gtcgtggccg aactgggact tgctgcggtc
    94621 gcgaccttgg tgtggggctt cctgccggat ggggccatcc ggagcgtcgt cttcgtggcg
    94681 gcgacgacca gctgggtgat gagcctggcg gtcaacctca acccatttat gaggtttgac
    94741 ggctactatc ttctggccga tggactggga attccgaatc tccaggaccg ggccttcgcc
    94801 ttcgggcaat ggcggattcg tgaactgttg tttgcgccag gcgcgcctcc gcccgaagcg
    94861 gtcgggctgt ccaacaggcg gatcctgatc gcttatgcct ggggtatctg gctgtatcgg
    94921 cttgtcctgt tcaccgggat tgcgctggcc gtctatcact atttttttaa agtgctcggc
    94981 ctcctgctgt ttctggtcga aatcgtcttc tttatcggtc tgccgatctg gcgggaactg
    95041 acgagctggt ggagtcgccg tgctgcctat gcatcgacgg gccggtttgc cgtcacgatg
    95101 acggtcgtcg ccgcgatgct gttgcttgcc tgtcttccat ggtccagccg tgtcgcgatc
    95161 ccaggggtgc tgcaggcagg gtcctatgca accatctatc ctccggcgcc ggggcggatt
    95221 gcatcggtgt cggtgcatgc agggcagtcc gtacgagcgg gtgacaccgt gctcgtactc
    95281 gaaaacccgc tattagccaa ggaggcccgg ttggcccgga cccaggtcga gctcttggat
    95341 ttcaggctgc aacgtcaggc cggatacgca gaggatcgtc tccaaggtca ggttcttgcc
    95401 gagtcgttac gatcgaaact cgtcgagctg gacggagttg ccgagaagca gcagcgcctc
    95461 gtcctgcagg cgcccatcga tggcgtggtg gttgatcagg ccgattcgct ctatcccggc
    95521 cgatggatca atgagaagct ggcggtcgcg tacgtagtga actctcgaac ggccatagtt
    95581 acggcccttg cgccggttga ggatctaggt gtgctggcag tcggacagga agcgctcttc
    95641 atcccaaacg atgtgacgcg tcaggccact cgcgcccggg tcaccgaaat tcgggacgtg
    95701 gacgaacagg atctttccgt tccctacctg gcatcaatct atgggggtgc cgtgcccgta
    95761 cgaaaagatt cgcatggtcg attgcaggcg gagcaatcgg tctatcgcgt agaacttgaa
    95821 gtgctggacg aggttgtgcg ggcggatcag gtcgtgaccg ggctcatcca cgtcgccagg
    95881 acgcctcaga gcctggttgc acggatgtgg gaccgagtcg tggcggtcat gatccgggag
    95941 agcgggctct gagcaggagc gctggaataa ctcaccgata gaggatcaag ttcgccgcga
    96001 cgatcactca tttgggctga ggcaacgagt atgttcgatt ttatcaaaaa gtatctgcgc
    96061 aggaatggtg agccatcaga gcggccatcg tccctcgcga ctccgtctgc cttcaagcaa
    96121 tctctcctat ccctcgagca acgattgatg tttgacgcgg cggccgcggc gaccgcggcc
    96181 gaggtgcaat ccgaacaggt ggctcaagat caggccgagg cggcggtctc ctcggagggg
    96241 actgacgaga gtacgacgcc tgagcagcag gaatctcaag agctactcca cgcgattggt
    96301 tcctacagtc ccggcgaatc ctcgacggaa gtggtcttcg tcgaccccac ggttccggat
    96361 tatgaatctc tgctcgcggg catggggccc aatgtggagg tcgttgtgct ggatgcctcc
    96421 cgagacgggg tcgaacagat cgccgagtcg ttaaccgggc gatcgggcat cgatgccatt
    96481 catctgatct cgcacggcga cgctggaaca ttgcaagtgg gcaccggcac gttgactgcc
    96541 gaatcgatgt cgactcgtta cgtggacgag ttagcaacag ttcagcaggc tctgtcggag
    96601 caggctgata ttctcgtgta cggctgtaat tttgccgagg gggatgtcgg ccaggcggct
    96661 gtggcccggc tggctgaact gaccggagcc gacgtggccg cgaacagcga tagaaccggc
    96721 cacatcgatt tgggtggcga ttggaaattc gaaacccagg ttggatcgat cgaaaccgac
    96781 ctcgcgatta ctgatgccgc gcagatggac tggaagggga tcctgacgac cgagactgtc
    96841 ggcgaccaat tctccttaag ttcttacgac aatcacgatg ggactcagaa gtgggcctca
    96901 gattggtccg aaaccgatgc cggcggcggt ggggcaagcg gcggggatgt gcatgtcgct
    96961 tcatcacaac tgaggatcga cgccggttcc gtaggaaacc aagcgtcgcg gcaggtcgat
    97021 ctgtcaggaa cgagcggcgc aatcctcacc ttcgattaca ccaactcgtt gtcgggctcc
    97081 gatcaaatcg agattcgcgt gtcagccgac ggaaagacgt ataccaccct caccgacggc
    97141 gtcttctccc ctaagtcgaa tgccggaagc ggcaccgtca agctcgacat ttccggttat
    97201 gcctccgcga gcacgacgat ccaatttctc gtcaccgggg tgggtggggg cgatagcctg
    97261 tctgtcgata acgtgcaggt cagctatgac accggcgccg cgaacagtgc gccgacgatc
    97321 agcagcaacg ggggtggaac gacggcgtcg gtcacggtcg gggagaacag cacggtcgtc
    97381 acgaccgtca cggctactga cgttgatgca gggcacaccc tgggctattc gatcgccggt
    97441 ggagcagacg caggtcgatt caccatcaac aacagcacgg gtcagctgag ttttgcctcg
    97501 gcacccagct atgaagcccc ggcggacagc ggcgggaata acgtgtacga cgtcacggtg
    97561 caggtgtctg acggccaggg cggcacggat acccaagccc tcgccgtgac agtggcaaac
    97621 gtcaatgagg caccgacaga cctggccttg tcggcgaaca cggtggcgga gaatgcggcc
    97681 aacgggaccg tggtggggac ggtgagcggg acagactcgg acaccggcga caccaggacg
    97741 tactccctga ccgacaacgc cggcggacgg tttgccatca atagcagcac gggccagatc
    97801 accgtcgcca accgatcgct cctcaattac gagagcgtca ccagccacaa cctgacggtg
    97861 cgagtaacgg acagcggcgg gctcacctat gacgagacct tcaccatcaa cgtgacggat
    97921 gtgaatgaag cggctcccag gatcaccagc aatggcgggg gggccacagc cctcatcaat
    97981 attgcggaga atgccacggc ggtcaccagg gtcactgcct ccgacgccga taccaggcaa
    98041 accctggcct actcgattgt cggtggggcc gatgccgcca agtttacgat caacagtagc
    98101 accggtgcct tgtcgtttgt gtccgccccg aactacgagg cgccgaccga tagcggcggt
    98161 aacaacgtct acgatgtgac ggtccaagtg tcagacggaa gcggcggcgt ggactctcag
    98221 gccatcagcg tgaccgtaac tgctgtgaac gagaggccaa cggacctgag tctctcggcg
    98281 aacacagtag cggagcatgc cgccaatggg accgtggtgg gcacggtgac cgggagcgac
    98341 cctgacgccg gtgacacgaa gagctacagt ttgaccaata cggcgggggg gcggtttgcg
    98401 atcaaccgga cgaccggcgc cctcacggtc gccaacagta ccttgctgaa ctacgaagcg
    98461 gcgacgagcc atgccgtgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    98521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    98581 cgacccgacg cccaatacgc tcacggtcaa cgtcaccgcc gtgaatgacc ggccgacgga
    98641 cctgagtctg tcggcgaaca tggtggccga gaacgcggcc aatgggaccg tggtggggac
    98701 ggtcagcggg accgaccccg acgccggtga cacgaagagc tacagtttga ccaatacggc
    98761 aggggggcgg tttgcgatca accggacgac cggtgccctg acggtcgcca acagtacctt
    98821 gctgaactac gaagcggcga cgagccatgc cgtgaccgtc cgggtaaccg atcggggcgg
    98881 cctgacctac gacgagacgt tcacgatcaa cctgacgaac gtgaacgagg cccccagcgg
    98941 gaccaatgcg accgtcacga tcaccgaaga tacggcgcac gtcctcacgg cggcgaattt
    99001 cgggttcagc gatgtggatg ccggggatgc gctgagtgcg gtgcggatcg acacgctgcc
    99061 gacggccggg actctgacac tctcgggcac ggcggtgacg gcgggccagg tgatcacgac
    99121 ggcggacctc gcggcgagcc agctggtctt taccccggcg gccaacgcca acgggaccgg
    99181 ctacgcccgc gtggccttct cggtgcgcga cagcaccagt ctgtacgacc cgacgcccaa
    99241 tacgctcacg gtcaacgtca ccgccgtgaa tgaccggccg gtgatgaccg ccaacagcgg
    99301 gagcagcgtg gccgaagggg gcacagacac gattacgagc gcagagcttg cggcggtgga
    99361 cgtggacaac tctgccgccc agatcagatt ctctgtcggg accggtcctg cccatggtcg
    99421 tctggagttg acgaccaggc cgggggtggc gctggcgact tttacgcagg ctgatctcgc
    99481 ggccaaccgc ctggtctatc gtcacgatgg atcggaaacg ttgagcgatc gctttacgtt
    99541 tacggtgagc gacggggcgg gaggttcggt gggcacgacc acaatgacgc tgacgatcac
    99601 ccctgtgaac gatgcgccga ccatcattaa caatgaagga gcggcgaccg cctcgatcaa
    99661 tgttgcggaa catgtgaccg gcattacggt cgtaaccggc gccgatgtgg atctccctgt
    99721 gcaggccttg acctactcaa tcagcggcgg agcggatcag gcgcagttta cgatcgacgc
    99781 ctcgaccggt gcgctgagtt tcgtggctcc tcctgatttt gaagcggccg ctgacgccaa
    99841 tggcgacaat gtgtatatcg tgcaggtccg tgtcaccgac agccagggcg gcacggcgat
    99901 gcaaaccatt caggtgaggg ttacggatgg ggcggaggcg cgcccctccg gcccggcgct
    99961 tcctccctct ctcattccga tgccttcggt acaggcgggc acatccgttc agcctcatga
   100021 cgaaccactg tccttcacaa cgcgacagga gccggtgaat gcggtgtcca tgaatgggac
   100081 tgatctcgcc gggacgatga gcccctatgc cggcagcatg ccgccgcgcg tcgcgcccca
   100141 ttcaggcgat gttcatcatg aggaagagcc acctgtcgcg tcacgatctg ttcagtcgga
   100201 tgacagggag ccccggaatt catccccgat ctcatccacg gacgcgatcg aggcgggcag
   100261 ggatggcgat tcggggccac agccctcggc gagtgaactg gtactgaata aattggatga
   100321 aatgatcttg tcgttgcggg aggcggtcgc tgtggaccgt gagccgcagt cgttgatggc
   100381 tcaagtgact gcgttgacgg gggcgactct ctctgtggga tttgtgctgt gggccgttcg
   100441 caaaggaagg ctcctggccg gttgctgcgc aaccattcct acattgtcgg cagaagggcc
   100501 tggtctaggt caacacctgc gcccacggct cttgacgaaa ccgtctcctc agtcgcaagg
   100561 ccccgtgaac attccctagc cgacttctcc aacctgacgt cccacacaac ggatacaacg
   100621 ctatactgca acttgggcct tgaccatagg atccgtggtt tgatgaaaaa accgctgagt
   100681 gtagaaatag acgcgaactt gtcttagaag tttctttggc tgacccactg gcagttagga
   100741 ttcttgccga tctcctctta tttccgctgc cattcctaga ctctcacaga attcgtcgag
   100801 ggccttaagg aactcgctcg ataatgcgtc acgaagtact atgtcccttt cattaacagc
   100861 ccagccgatg catggtcctt ttgtgatggg gtaggggaaa ttaaagtcct tttcgctgta
   100921 tttttcgcta gctttcagtt ttgctagagg cattcttatt cttgcaaaca tcctcttcgc
   100981 ttgtatgagc cgaggatgtc ctgttgctgc tgcaagcgtt ctgagtattt cataggctcc
   101041 caatacccat aaatacgaat gggtgagaat atcgtctagc tcaaggggtt gttcaggaat
   101101 ctggcggagt cgatttatcc aaaaggccgt atccttctta acaatttcca actcaaaccg
   101161 agcgagtttt tgaatcgtca tcacacagcc catctgttcg ggagcgttac gaggaaaaag
   101221 attcgccaat ttatggctga tacggaccca tttcgattgt tgctcgaaga gctgtgagtg
   101281 aaccacggat agcaggaaat tcaatacttc tttttctgct tgtaaccaat gctctaggct
   101341 gttgccgtgg tgacgaccac ttttctcaaa aatattgtgg gcacgttcgg cgattagccg
   101401 ctcaatttga gatgcctgac cctgcaacgt ttcccattca gccttccaat cattcatgag
   101461 cagcagttta tacgatctgt gctagacgat acttcagagg agagcgccac ggcaccccta
   101521 gtgtaccctg ccaatgtttg gatcagagca gctgagtcct aaccactgga aagtttggtg
   101581 gcgatgatcc ggattgaact ggtgaccctc ggcttatggg ttcattaagt agatgcgcta
   101641 cagcagcggt ttcggcatga aaggccctcc tatccgcgcc cttctacctg cttcgtaatc
   101701 agaaatacgg ctcggcagtg ccccagtggg ctacccctgg gacgatggtt gggacggtcg
   101761 cccattcggg taccggtact gtgtcatccg gtcttatcgc aagctcgagg gtgcagcctt
   101821 cattgcaatg ggcacgtgcc gacgcacgga aggctccagc cgttcctgct aaggaacacg
   101881 tctgcgggat ttatcttgag gggtccattc gcagggtcgg ggcactcggc ctgccccgtc
   101941 gggcaatctt tgaataggaa gtcaaacgcc tccgtcgatg gactgttgca ttgggtatca
   102001 tagcatttgt ccccaatcca ggcggggcca cagtcgaaat tattactcac gggatagttg
   102061 atggcagggt ttggataacc cccaaccatt tgaaaatagc agcccctaag atcgttcctt
   102121 atcagcggaa cgacgatatg ccatcgcgtc ggagataggg tctgccacaa ccacatcgca
   102181 tagctcattt ccccggcgtt acgcatcgcc ttttgctgct catcagttgg tgcagtgcct
   102241 acgatgggcg cggcaaacgc tttcaattgt gaccaatcgg tgacgagctg gtcgtgagta
   102301 tcattgttgc cgttcagcgc cgtgttgtac atgatcgcga gcttgccttt cacttctccg
   102361 accgccaggt tagggtccga aggaaagcca ccgtgcttac caatatagtt agtgacttga
   102421 ttgaagacat ttgaaaacac tggcgggaga ccaaccaccg acccaccgat ggccaatccg
   102481 accccgagga acaactgcgt cagatttgcg gctatttggt cggcagggtc cacgttctgc
   102541 aattttgcgg agactgaatc gacatcgaaa tccgtctctg caaagatttg cgtcagcagc
   102601 gtctggctgc ccgtgggacc cagtaaccaa tcattggcgg tttggacgta gctcagctcg
   102661 gtcttgattt gtcccacgac cggggtccag ttggcagcgg tgcatgtcgg tggaggcgac
   102721 aacgcgcgca agtttccctg cagggtcgca ataatactgg aatcagtcag ttcgtcgtag
   102781 tggctgcgga ggtctgtgcc atatgtgccc ggcgacgtgc tgatattggt gctgaggtac
   102841 tgatagcagg cattctgatc tccgggaaac ggcgggaaac tgaactgtgc ggcgcgagct
   102901 tgcccgccaa gtacggtcgc aagcatcatc atccctggga gcagcagctt acgtgtccag
   102961 gcgttgcctt gacgtgggcg aatcagcatg tttttaagcc ccctttttag agcgggaaga
   103021 tacaacgatc tcggttagct gctagcgcaa cgtataagaa cgaaagatcc tttttgcgag
   103081 agcaagtatg ggagatggac ccgaggtagt caaatacgcg agacgtcggt ctaggatcgg
   103141 atcttggagc gattcatcgg tccgacacgc ccgcgtgtgg ggcggtttac cgggctactg
   103201 tcaggtggcc gcctgactga catattacgg cggcatgcca atgggcaatg caatctctct
   103261 taaattggaa acaggtcatt ctcattgagg gaaagctgac attgcgctga attgcatcgc
   103321 aaataacggt ttcgcgccgg cgcgggacac actgcgcact ctgcaaatca gatggaagag
   103381 cagctgagtc ctaaaccgct ggaaaatttg gtggcggtga cccggattga acgggtgacc
   103441 cgcggcttat gagtccgctg ctctaccaac tgagctacac cgccactgga ggatgcgtga
   103501 ccggtcgaag tgaaccgcaa taatagccga agaagagttg aagtgtctac cgactgatgc
   103561 acgaaattct ttctcgatcg gacgagctct tccgccaccg gttggcggga tcccgtgagc
   103621 tcggggggcc tggggatttg gccctcgata tgattggctc atgctgttcc cgtatcatcc
   103681 gtatcctatg aggttggcca cggatagagt ggcaggaggg gcctgatgtc gctagactga
   103741 caggcgtgct ccacgactga cggagccaag gtagccattc atgtcggacg tcacgattcc
   103801 atcggtgccc aagctgttgc ggcagcttct ggccgctctg cgaggggagt ggtggtccct
   103861 tcgcaagttg aaacgccgga cgtcctcaca caagggagat cgatatgttg accggagtgt
   103921 ctctgcagac ggcccttccc tggctcttga gtatttccac aaccgtaggc atcggcgtgt
   103981 tgcgggtcgg gctggtgttc gtgattgggt atatcgcgat tcgatttgcc cgtttggggt
   104041 tgcaacaact cgaacgggtg atgcttctca cgagtgacgc caaggatcaa cagtccggaa
   104101 cggctcagaa acgcgccgcg acgctcgcgg ggatcctcag gaccatcgcg ctcacggcga
   104161 tttgggcgat tgtcatcatc gaatcactgc aggtggtggg gttggacatt gcgccgattc
   104221 tggccggtgc cggtatcctc ggtctggccg tgggcttcgg tgcacagaat ctggtgcgcg
   104281 atctgatcag cggattcttc atcatccttg aagatcagat ccggttggga gatgtggccg
   104341 tgatcaatgg aaccggcgga caggttgaga ccatcacgtt cagaacgatt tccttgaggg
   104401 acttttccgg cgtggtccac gtgtttccca acggcggcat caacaccctg tccaatatga
   104461 gcaaggactg gtcggccttc gtgctggaca tgggagtggc ctataaagag gatacggatc
   104521 gggtggtggc ggtgatgcag gcggtcggtg aagaattgcg ccacgacgag gaacttggct
   104581 cgctgatgat cgagccgatt gaagtggtgg gtgttgaaaa ttttgccgat agtgccgtaa
   104641 cgattcgcgc gcgcatcaag acacagccgc tcgaacagtg gaaggtcgga cgtgaatacc
   104701 ggcgacggct caagaaggcc ttcgacgcgc agggtatcga gattccgttc ccgcatcaga
   104761 ctttgtgtat gggagaggcc agtccgccgt tcaaagtaga ggtggttgca ggtctggggc
   104821 ttcagaaggt ctagcagaat gcggaaacag tccggttgag tccgatggcc ctgcttacca
   104881 ggagcatgaa ccaggcgtct ccgtggttgt ccgggcaacc ggtggccggc cgctgttagg
   104941 ccgcccggcg agtctgccgc tcaggcacga tgtcgctgtg agtgggggaa gcggcagacg
   105001 aggcggagat cagttttctc cgttccgagt cactctctaa tcccatggcc caggccaata
   105061 accaaataag tgtcacaatt gtgacgagac caatgatcgt aaccatgtgg cacctcacgg
   105121 ctgaatacta cagaagccgc accggccgta ttctgtctcg atacggccag ccggctgctg
   105181 agtgacgtct ctcgaactca ccgggcgatg cgtgctcttc gctcacgtca tgtgctggtt
   105241 gcgacgatag tcttctaagc gccggcgaat gatcccctcc gacagcgtgg gatagtaccc
   105301 tttgagggct tgccgcccgg tggtcgcggc agtggtgtcc cggtgatgcc ggaactctct
   105361 caaccgttgt tcaattcgct gtttcgccgc agcagagaac gcctcggcca attcccacac
   105421 gctggtatgt ccgtcgagcc tggtctgtcg ttcggcatag agcaccgtgg cgagcatggc
   105481 aaggagatct tcaccgacgg attcgatttt gttctgcaga cgctgctcgt ccttaaatga
   105541 ggcctggaat cgcagcaagg cgtagaaaat tgctcgcgcc agccgccggc tggttcgttc
   105601 cacatagagg gccgccggcc gtacctgggg gtgggtgatt tctcgacggt ccggcagagg
   105661 ccgttttcgc cattgccgga ggtaccaggg caggtagaag cgcatgagcg tcatcgcctg
   105721 ccgggcttgt tcgagtatgg aaagaccatc tgcatagaag cgcttggcac gatccagatg
   105781 agggctcaag ccttcgcgga ccacgaaggg gcgcaagata tcggtggcgc cttcgccgat
   105841 tcgatacatc tccgcatcgc gaacgagttg ttcgatgccg aaggcggctt ccccgcgcgc
   105901 gtgcttggag tcggcggttt cgtaccccat ccctccgaaa atcgtttgcg cgtcacggat
   105961 gaaccggatc gtgtgttccg aacagaaaat cttggcgacc gcggcttcga tgcgaatgtc
   106021 gtagcggtgc tgatcggcca ggcgccagac gagcagcgcc agtgcctcca tggcgaacag
   106081 gtggccggcc atgtctccga tccggatctg ctgcgtctgc cgctctgcca acggcttctg
   106141 aaacgtgacc ctgctattgg ctcgatccaa ggtcggttgc caggcctgtt tggccatgcc
   106201 caggcagatg gcgggaatgc tgatgccgcg tcccacgttc agaatcgtca aagcatactt
   106261 gagccccttt ccgatgtcgc cgatcacatg ttcgatcggt acctgcacat tcttcaaggc
   106321 gatatgggcg ttctcaatcc cgcgacagcc ttcaaactga cagcgttgac gcaccagcac
   106381 gcctggtgtc gccatgtcca aaataaaggc cgtgtgaacg tgatcgtccg cccgctgccc
   106441 ctgcggtgcc ggaatccatt cgacctttcc ctcacactcc acccgaagag ccggcacgcg
   106501 ggcgacgagc gtgaggtacc gggcaatcgg cccgttcgta caccagagtt tttcgccgtt
   106561 caccagaaaa tgggtgccgg aggaatcgag gaccgcctcg gttcgcacgt tggccgcatc
   106621 cgaaccggtc atcggttcgg tgagggcaaa ggccgaaagg gcctccttcg cgaccagcgg
   106681 gagatatttt ttcttctggt ctgcgtttcc gaagaggagg atcggcatgg cgatgccgat
   106741 ggactgcggc actgccaccg tgagcgccag aatgttgctc cagctggcga tcaacgtcag
   106801 gacccggccg tagttcgtat aggacaagcc gagcccgcca tattctttgg gaattttcat
   106861 gccgaaggcc ccgagcttga acagcccctg gatgacgcgt tcgggaattt ttgcctcgcg
   106921 ttcgatttct tgcgggtcca cctcgtggat tagaaagtgt tcgacctgtt tgcagaaggc
   106981 ctcgccggcg gttttctctt ccggcggttc gggaggtgtc agcaagaggt tgaagtccgg
   107041 ccggccgtca tagagaccgg ccaggaaact ctttccggtg aagcgctcgc gggcttcttc
   107101 gatccgttca aaacctgtaa agagggtgct catgtggtac ctcacccgat ctgctcaagc
   107161 aggatctttg aattgtctgt cacgatattg ggccagggtg cccgccaacc tggatttcag
   107221 ttccaggcgc ttgggtttcc cggtggaggt gtagggcacc tccgtaccca tcaccagcac
   107281 tttcggttgt ttggcaaacg gcaaccgtcc tcggcaatgg tcgcggatgt cgacctctgt
   107341 cgggggcgat gccgggtctt tcggcacgat ataggcggcg atttcctcgc cgtagtagcg
   107401 attctcgaag gggagggcca tggcgaactg cacggccggg tgtcccttca acacgtcgtc
   107461 gatttccaac ggggaaatat tgacaccgcc gcggacgatg agttctttca accggcctga
   107521 aatgaaaaaa aacgggcgtc cccggtcgtc ggcgatgcaa aagccttcgt cgcctgaacg
   107581 gaaccaaccc cattgaaagg cggcttcgtt ggcgtcgtcc cgtttgaaat agccggcgca
   107641 gactgtgcgc ccgcggatgc agatctcccc gcgtgcctgc tcagcaaccg ccttcccgga
   107701 ttcgtcgaga atggccatct cgttgtgacg cagggccgtt ccgatcgagg ggaaatcaaa
   107761 atctgtgagc cagtgacgat gttcttcccc tgtgaggtcg ttcgggagga aacaggaata
   107821 gcaggttgtt tcagacagac cgtatccatg gcggatcggg aatccaaaac gctcttcgaa
   107881 gcgcagggcc gtgtccttca gcagcggacc ggcgccgcag atcacaccac caaagcgatc
   107941 caggcgatag gcggcgaggt cttcgtcggc atccagtaga aactcaagca atgtggggac
   108001 cacgctgaca ctggtcgcct gctcatcgtg gagccgccgc cagaagctgc cggtcttgaa
   108061 cttccggttc aagacgacgc ttcccttgca ataaaacgga gtcatcaagg tgacgacgat
   108121 gccgttgaca tggtggatgg gcaggacgca catcaaacgg tcggtgagac cgaatccatg
   108181 ccggtcggcg atggcatccg catcgatcag aagattggcc accgtcaaga tgacgccttt
   108241 cggcggcccg gttgtcccgg atgtgtaaat gatcaaggca ggatcgtcga gtcgggaggt
   108301 gcccggggca aagcgggctc caacggcgtg gctgtttttt cggctctttc ctctggtctc
   108361 cttcccgtca agaatgccgc cgtccccgag ggcgaccact tcgcgcaagg ccggcagcat
   108421 ggactgaaga gcgttgagtt cctcgtagac atcctgccag cagagggcga ccgaggcctc
   108481 cgaatgttcg aggatatacc gcttcttctc gggtgtttct tcgacattga tcggcacgac
   108541 ggcgactccc aacgtccagg ctgcgaagta gatcagcgcc gtcagatcat ggttgaacag
   108601 cacggtggcg atccggtcgc cgcgggtcag gccgaacttc gcatgaaaca tctccgccat
   108661 ccgttcgacg atcgtgccga attccgcata gctgtaggtt cggcgcaggt tccggtcgtc
   108721 gtcgcaatag gtcaggaaat tcctggtaac gagtcgcggg tcgtacaggc gcgatcggaa
   108781 gaactcggcg aacgaggtcc agtggagttc cgaggcacat cccggcgcct cgcgggcctg
   108841 ttcgatccgg tcatgaacgt cggtcaagag gctcataggt cctcggtgcc ggccctgtgt
   108901 agagggctcg tggtctgatc agccggttat gttcctgctg ctccattaca tgtgcgcacc
   108961 agccggtgat gcgggaacag acgaacaacg gtgtggacaa ttcgcgcggg atgcccatca
   109021 acaggtaggc cacggccgta tagaaatcga gattggggtg gagtcctttt tcctcccgca
   109081 tgatgcggtg gagggtggag gcgatgtcgt accaactgtg gtcgccgcag aggtggctga
   109141 gccgttcggc gtggcgctgg atgatggctg atcgtgcgtc gccttgccga agcacgcgat
   109201 gcccgaatcc catcactcgc cgtttctgcg cgagcgcctc tcgcacccag gtctcggctc
   109261 gctccgggct gccgatttcg atgagcatcg acgccaccgc ttcgttggcg cctccatgga
   109321 gcggtccttt caatgtcgca acggccgccg tgatcgcgcc atgcagatcg gtgagggttg
   109381 aggcggtgac gcgagcggca aacgtggagg cgttaaattc atgctccgcg tacaggatga
   109441 gcgacacgtt cagcgcctgg gccatggccg tgccggtttc accgtccttg tggcctgcca
   109501 ccaggtgcag caggtgctgc gcaaaatctc gactctcgtc ctgtctcggc gggagtattc
   109561 cggacatgac tcgataacta tgcgcgatca gcagcgggat ctggctcagc agtcgcatgg
   109621 atttgcggag attggcctca tgcgatccat cctgcgcgtc ggggtcgctg agccccagca
   109681 gcgaaatccc ggttcgcagc acgtccatcg ggtgcaggcc cggttgcagg cggtctacaa
   109741 aacgttgtac tagctcaggc aggactcgat ggcctctcaa gagtgtggaa aaaccatcca
   109801 gctcgttacg ggtggggaga tggccgatca gcagcaaata ggccacttcc tcgaagcggc
   109861 tccattccgc cagatcgccg atggcatacc ctcgataacg aagtcctgcc tctccttcat
   109921 ccacctgaca gagggccgat tctcccgcga tcaccccttc aagtccgggg ctatagaggg
   109981 ggcgctccgt ggttttcgtt ccgggctgtt cttgtatcgc ctggttcata gggtctccct
   110041 gtctggttct gcccatgctc gtatcgaagc agactataga gctcctgtcg cgtcatcatg
   110101 tgatcaagcc aagcccgttg agagccgaag aatctgagtt ctcgcagcag cttctcaaca
   110161 gcaagggctg cgacccggag cgtgctgacg ggaaataaca cgccgcgata tcccaacgtc
   110221 tcgaattcgt ccacgctcaa gtaaggtgtc ttgccgaact ccgtcatgtt ggcgatcaag
   110281 ggcaggtgga cgcctgcccg gttcatttcc aacgcaaagg tgtggaattc gtccgcggag
   110341 agcagggcct cggggaagag tgcgtcggcg cctgcgttgg cataggcaac gcctcgctga
   110401 atggcagcct gcagcccatc gaccgtccgt gcgtccgtgc gcgccacaag gacgaaatcc
   110461 cgatcgcatt tcgcctccac ggctgcggtg attttggcaa ccatgtcagc cgttggaatc
   110521 agtcgcttcc ccgacagatg gccgcatttt ttcgcctctt cctgatcttc gatctgcatg
   110581 cctgccaatc cggcccgctc aaattcctga acggcctccc gcacaaccga aggcggccca
   110641 tagccggtgt ccgcatcgac gatggtggga agcgccaccg accgggcaat cgccgaggct
   110701 tccctcgtca tgtccgtcaa gctgatcaat ccgatatcag gcaggccatg ggccgccgag
   110761 atggcggctc ccgacacata cacgatctcg aatccggcgc gctcgatctg catggccgtc
   110821 agggcattac aggccccggg gatggccact gtcctgccgg ccagcagccc acgcaggcgt
   110881 tgtgcttttg aaggagtgga gtggttacgc atcacgatgg ggcaccctca acagaggcat
   110941 cagtgtggcg atgtcttttt ctcgttccaa tgtccacacg aaaccgatga gcttctcgac
   111001 ctgcgcgaga ccgattcgtc tcgatgcgag ccggcgcagt ttgtcctcca catcctggtc
   111061 cgacatggga ttgccgggat ggcccagggg gacgtccaca tgcctcatgt acaccgttcc
   111121 cgtcgtcgtc ctcagtgtga ggcgggtcgg catcgctccc gggtaacggc ccgcgaattc
   111181 cggctgcggg acgacacgca ccttcttcat gagctcgtgc agcgcagcat ctcgtagccg
   111241 gtgagggtcg aatgaatgca acgtgacgcg accatccagc aacgccaccg cgacacaata
   111301 ggggaagatg tgaacgcccg tttcgcgcgt ctacgggtgc cacttctccg gatcgcgccc
   111361 gataatttcg atcgcgacat cgtaactgcc gatttcaatc tcgctcagac gctggattgc
   111421 atcggccccc tccgccttga tcacctccgc tcgcagggcc agcgccgctt cgaccgcagt
   111481 ctgggcatgg tattccaccg ggaaatgttt gatgtaggaa tcgagaatct tgaacgaagc
   111541 cagggtgtcg tcgggagccc gctctccacc cagtctcgac agctcgaatg gtccggacac
   111601 cagcttcatg aagccctttt cgccttcaaa gatgggggcg gggcctgtca tgcctcgttg
   111661 ggccaggatg gccgcgaata ggccgttgcg tgcggcattc gagaacgcac aggctttcca
   111721 catggacatt tcaccgactc tggtttgccg gagtgccacg ttcgccacgg cggccaggtt
   111781 gatggcctgc gtggtctgcg cctccgtcag tttcagcgtt ttggccgcgc cgagcgcgga
   111841 ggagatcgat ccataggtca catggtccca tccacgggag cgtaacgcgg cggcgtcgca
   111901 gagacggcat tggatttcat aggccaaggc gatggcctgg atgacccgct tgcccgaagc
   111961 atgggctatt tcgccggcgg cgaggattgc ggcaaggttg tccgaaggat gggccggctc
   112021 ttttgagagg taggtatcgt tgaagtcgag gtagcgaacc aacgcgccgt tggcgaaggc
   112081 ggccaggtcc ggcagggtct tgtgggacgt cccccagatt gttgcaccac cgggaacttt
   112141 ggcggattgt gccacgtgac gcgcgatccg gcagggggtt gccttccagg cgccgaaggc
   112201 acaggcggcg ctgtcgagaa tccgtcgctt gacctcatgg accacgtcgc cgggcaggtc
   112261 gtcgtaacaa agggcctggg tataactcgc aagacgatcc gccagcatga tgatcctccc
   112321 gacaacaggc cgttctgtca gcccagtgta tgtgcgagat cctggagccg ttgctgttcg
   112381 gcgatcatgg cgatcagctc cggaatgccc tctcccttga cggccacggt gcgcagcacg
   112441 tgcgggcacc actcgcgtag atcgtgtact gcagtatcgg cgccttcacg atcccctttg
   112501 ttgacgacca cgatatccgc gacctccagt aaccctgcct tcatggcttg cacgtcgtcg
   112561 cccaatcccg gcgcgaccag ggcgatgacg gttcccgcca cagagacgac ctcgatgtcg
   112621 ctctggccga cgccgaccgt ttcgatcagg atcacgtcat acgcggccgc ttccaggact
   112681 tgcagcgcat cgcgagtcgc ccgcgcgagc ccaccggact gaccgcgggt tgccatgctg
   112741 cgtatgtaga cccccgcatc gtccgcgtgc cgctgcatcc tgatccggtc acccagcacc
   112801 gctccgccgg taagagggct gctgatatcc acggccagga cgccgaccgt tttgccgagg
   112861 cggcggtagg cggcgatgag ttgatcgatc aacgtgctct tccccgtgcc gggataaccc
   112921 gtgatgccga tcaccatggc gcgcttctcc ggtggcgtca ggcgtttcaa cacggcggct
   112981 ccaaccggct cctggttctc aatccaggtg atcacgcgtg ccacagcccg gaccccaccg
   113041 gccagcactt gcctggccat ggtctcgaca tcctcccgtt tcgataatcg accgacgcac
   113101 gcgtgattca tgtcattccc gccacgcagg ctgtggcttt tcacgatgat ggagttgcga
   113161 aagccgtgag ttcagcggat gccgcacggc gtcaccgagt tgttgcgcac aggcggcaac
   113221 cagcagttca tccaccggca cgttggcgcc ggaggtcttc agcgcgaaga ccagatcttc
   113281 cgtcgcgaca tttccggatg cgcctggggc gtagggacag ccgccgaggc ctccggccga
   113341 agcatcgaac gccgtgattc catagtccca ccaggccgtg agagcgttgg cgacggccat
   113401 gccataggtg tcatggaagt gaagcgagag gcgagccggc tcgacatgcg gcagcaccgt
   113461 atcgagcaat ctccgtatgt ctgtcggtga agccttgccg accgtttcgc ccaacgagat
   113521 gtcgtcgacc gcgaggtcga gcagccgttc catcacgtcc cgcacctgcg tgggtgcaat
   113581 gcggccttca tagggacaaa acacgaccgt ggacaggtag gctctcaccg ccatgccgtc
   113641 tcgtttcgct gccgccacaa tcggtctgaa gcgcacgagt gactcctgca cggtcgcttt
   113701 gatgttatgg cgggtaaagc tgttcgaggc agccgtgaag acggcgatct tgtcgacgcc
   113761 tgccgctcgc gcccgttcga ggccgcgttc gttcggaacg agggccgaat aggcgacacc
   113821 cgggatgcgg tcgatggcgc ggaggacctc atctgaatcc gcaagctgcg ggatggccgc
   113881 gggtgacacg aaggagcccg cttcgatctc agtcacgccg gttcgtgaca gggcgttgat
   113941 taaggcgatt ttgcgcaggg tcggcacgaa ctccgattcg tgttgcagac cgtcgcgcgg
   114001 agacacctcg gtaatccgga cgaaggcttg ccgcgagtta tgtaggccgg cttgcaacat
   114061 ggtgttcctt cgctctctcc cgtgctggct gctgttcccg tcccttggcc cactgtggtg
   114121 gccgcttctc cgcaaaggcg cgaagccctt caatcgcctc ggcagcacca cgagcgcttg
   114181 cgttggcctc tgcgcaggtc accacgcgct cttgatcagg caacgggcgt atcctcagga
   114241 tcaacccttt tgttgccctg gttgcgcggg gagccagctg cctgatggcg tcaatgactt
   114301 ccgtgatgcg atcgtctaga tcgctttgag gcacgacgtc atgcacgagg ccgaaccggt
   114361 gggccaccga tgtggtgaat gtttcgccgg tcagcgcata ccgtcgaaga aacgattcgc
   114421 ctgccttgcg caggagaaag ggtgcgatga ccgccggaat caaaccgagc ctggcctcgc
   114481 tgagcgaaaa catcgcatct tccgccgcga cgacgatatc gcagaccgcc attaatccga
   114541 gcccgcctcc gaaggcaggc ccttgcaccc gggcgatgac cgggcaaggg cattcgtcga
   114601 tcgcgcgaaa catgcgggta agacgatggg cgtcatcctg tgcctgggct tccgagatcg
   114661 gtgacgtgga ttgcatccac tggagatcgg ccccggcgca aaagaccggt ccggcgctgg
   114721 atagcaacac tcctctcagg gacggctcct gagccgattc gtcgaatgcc tgagtcagtt
   114781 cacttatcat ccggctgtcg aaggcgttgc gccgttcagg cctatcgagc gtgatgcagc
   114841 ggatgtcgcc gcgtgattcc atcgccaggg tcgtatatcg acgcattcgt acctcctcag
   114901 ccgccacgat cctcacatac gaaacaccgg cacatgggtt tctctggccg gaacggacgc
   114961 ggccagatcc agacagaggc ccaggacttt tcgcgtctcc acgggatcca gaatgccgtc
   115021 gtcccagagt cgcgcgctgc tgaaataggg actgccttcc tgctcatatt gcgaccgggt
   115081 ggcctccgtg attcgccgct gttcttcatc cgacagccgt ccctgttcac gcgcgcgctg
   115141 ctgttgcttg acggtgacaa gaacctgcgc ggcctgttgc gcgcccatca ccgaggttcg
   115201 tgcgttgggc caggtgaaga ggaatcgagg gccatagcca cgcccgcaca tggcatagtt
   115261 gcccgctccg tgggaggcgc cgatcaggat cgtgaactta gggacttcgg cggtcgcgac
   115321 ggcctgcacc atcttggcgc cgtccttaat gatgcctctc gtctcataat cttttccgac
   115381 cataaacccg gtgatgtttt gcagaaacac cagcggaata cgccgttgcg cgcagagctg
   115441 gacaaagtgc gcgcctttca gcgcggactc cgagagcagc accccgttgt tggcgacgat
   115501 ccccacgagg tgacccatcc agtgggcgaa accacagacc agcgtggcac cgtaccctgc
   115561 tttgaattcc tggaagcgac tgccgtccac gagccgcgca atcacctccc ggacctccca
   115621 tttctgacgc ggattcgcag gaatgattcc gtagagttct tcggccgggt acaggggctc
   115681 atcgattttt tctcgacgaa ttctcggagg ccgtcgcccg agcgtggcaa tgatcgatcg
   115741 gcagatgtcc aatgcatcat ggtcatcctg cgccagatga tcgctgacac cggataggcg
   115801 ggtgtggaga tcggcccccc cgagctcttc actggtgacg tcttcgccgg ttgcggcttt
   115861 gaccagcggc ggtcctgcca ggtaaatcgt gccggttccc tggacgatca cgttttcgtc
   115921 gcacatggcc ggcacgtagg cgccgccggc cgtgcacatg cccatgacca cggcaatctg
   115981 ggcgataccg gctgcggaca tccgcgcctg attgaagaag atccgtccga aatggtcttt
   116041 gtcggcaaag acctcagcct gcatgggtag aaagatgccg ccggaatcga cgagataaat
   116101 cgccggcagc cggttctcca aggcgatctc ttgcgcccgc aaatgtttct tgatggtcat
   116161 gggaaagtag gttccccctt tgaccgtcgc atcgttggcc gcaataacac agaatcgtcc
   116221 cgacacggag ccgatgccgg tgatcagtcc tgcggccggg acctgattgt catacaagcc
   116281 aacggcggcg agggggctga gttcgatcca gggcgaccct cgatcgagca gggcggcaat
   116341 gcgctcgcgt gccgtcatct ttccgcgttt cttgtggagc gccactgcgt cggccggtcc
   116401 gcccgcttgc acgatggcca ggtgcttctt caaatcggcc gtcaggcttt cgtactgtac
   116461 ctgggcacga cgaaagtctt ccgatgtctt attcacggtg ctcttcagga cgcgcatctc
   116521 ggacatcctc tcaacgctcg actcgaagtc ccttcacgaa atcgacgatg tcgtgcatcg
   116581 gcgtggccgg gcgaaataac atctgtacac ccgcggcctt cagtcgcggt acgtcgtcat
   116641 cggggataat gcctccgccg aacagcgtga cgtctcccgc gccctgctct ttcagcaact
   116701 gcagcacgcg gggaaagagc gagttgtggg cgccggaatg gatactgaga ccgatggcct
   116761 gcacgtcctc ctgaatggcg gtggtcacga tctgctccgg cgtctgatgg agcccggtgt
   116821 aaatgatctc cactccggca tcgcgcaacg cccggacgac gagcttgacc ccccggtcat
   116881 gtccgtccaa tcccactttg ccgatcaaga ctcgaatggg tgtcgtcgct attgccatac
   116941 gttcacctct tccagcacat accgtccttc cgtatccttc ctgaga
//
LOCUS       DAORRQ010000005       101382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_5_length_101382_cov_68.817571, whole genome shotgun sequence.
ACCESSION   DAORRQ010000005 DAORRQ010000000
VERSION     DAORRQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 101382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 101382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101382
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5_length_101382_cov_68.817571"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..609)
                     /locus_tag="PK224_02785"
     CDS             complement(25..609)
                     /locus_tag="PK224_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14692.1"
                     /translation="MIADLVTVGFVRSRSEQEHRRHYRTAEIDLIPYGIANGPDEVLV
                     WRKSGNRMNLAGFWDVFEHAVPVELTKSLTIPIIPLWLFSVLKIFAYLDRKFPRDLRD
                     LAYVLEHYESLEMGERRFEIVGAADNVTYENAGAYFLGTDIRTNLSSMAALNMVRDFL
                     DGVTDEYHPVINTVLREESLLESGRLRIPTQAGR"
     gene            complement(788..1870)
                     /locus_tag="PK224_02790"
     CDS             complement(788..1870)
                     /locus_tag="PK224_02790"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HRB14693.1"
                     /translation="MTKLLAQESKIVTSSLEQLKAVHPLRIVNVKREGEQGWDGELTI
                     KTTGETYRYLFEVKTHLRPQAIHHLLIRAHADRKRWRKATELLLLADYINPVLANQLK
                     QAGVNFIDTAGNLFLKRSPGLYLYIEGKRPPLSPKDKPTRLFQPSGLTLLFGLLVEPE
                     SINRPYRDLAGENGVALGTVGWVKRDLLEQGYLEPTAKDRVRLIRRKELFDRWVQGYA
                     SRLRPKCFAGEYRDLRNDLPTVAKTFGHYAMQQALSWSLTGGFGADVIIHHYQGNSLT
                     LFVESWQEKEALKNLKWLPVSGGPITILKSFSPRVIQKPESQHRIPAAHPILIYAELL
                     HHGTDRDRETAQLMYKEFLEDSIAKD"
     gene            complement(1972..2289)
                     /locus_tag="PK224_02795"
     CDS             complement(1972..2289)
                     /locus_tag="PK224_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14694.1"
                     /translation="MARSRRPELEKDNPTAPQLYVGPRRRRALSRATTPSRPAAQDEI
                     AAAMHTLISAAEEFVNGVPYVQELDKERNALLDAIAQAQTLLSAEHVPVKRPARKAKD
                     RNS"
     gene            3325..4701
                     /locus_tag="PK224_02800"
     CDS             3325..4701
                     /locus_tag="PK224_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB14695.1"
                     /translation="MRGWHRFFSGGTSKVAHNFHDAFQRAGVELCPLQHPPSGPGLVI
                     FQECDPGLQDFLQDARQAGLHRIIAIRLPPGGLTQSVVWRLLRAGACDVLDWDDPDRT
                     AHAVAARLVRWKTIDELVTSSEVQDTLVCRSLAALIVLRQLVEAAYFTDSMILLMGES
                     GTGKELAARLIHSLDQRANKGDLIILDCTTVVPELSGSEFFGHERGAFTGAITARDGA
                     FSRANGGTLFLDEVGELPLGLQGQLLRAIQEHSYKRVGGNTWHRTEFRLICATNRDLK
                     KDIAQGRFRSDLYYRIAGDLFTIPPLRERADDILPLARHFTRSLVPDLDPPEFDETMR
                     EYLTLREYPGNIRELKQLIMRIMSHYVGFGPITAGCLPEGERPEAVEEQREWSNQEFE
                     RCIRSAIARGFGLKEIGRMAENIAVRIAVGDEQGSLQRAAARLRVTDRALQLRQATAR
                     FPNGQSEC"
     gene            complement(4873..5709)
                     /locus_tag="PK224_02805"
     CDS             complement(4873..5709)
                     /locus_tag="PK224_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRB14696.1"
                     /translation="MIIDCHCHAGAGDGLTGPWDTRAPLDKYMRRAARAGINRTALLA
                     AFHSDYRQANREVARIVASRPERFYAFAFVHAERDRGRIHSMVHEAAHRFGFVGIKVH
                     RHDARITREICEVARSFRLPVLYDVMGEVAVCELLAQDYPDVNFIIPHLGSFADDWRA
                     QLALIDHLARHPNIYTDTAGVRRFDLLDQAVSRAGAKKVLFGSDGPWLHPGVELAKIY
                     ALRLPDNEEDLILSRNFLRLISSIRRYPVRSTGVASRAPDGIGISSEVHDPWAGEQFA
                     CC"
     gene            complement(5909..6175)
                     /locus_tag="PK224_02810"
     CDS             complement(5909..6175)
                     /locus_tag="PK224_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14697.1"
                     /translation="MNEGYMDERGNTGEFRGRYFKHLGRAILNAYWGLKNYVRPIQHN
                     GKVVARSVQGRVALLKRNIAGAAPYANDVLPFNPRFTLIQVAKQ"
     gene            complement(6198..7661)
                     /locus_tag="PK224_02815"
     CDS             complement(6198..7661)
                     /locus_tag="PK224_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14698.1"
                     /translation="MREKLSFEVEPFSFQEFRNQPNRCEGEFQTGSGERASYEIYQAD
                     ESEDSLEGFVGSEHRDIGDLALGQRPTSIIYDESGKRLTFGEMIALAGDYFATYFQMA
                     GLSRTQEGRANIAFARWRALDMPKQQEPKVSDKVKDAVRVRYFVLASQNISHFSAGGT
                     AWSTYIASHSAAITDAFFAGEQSDAKTWQRAISKEGFACHFLTDIFSAGHVRTPRVAI
                     RHWYGKHYPDSSQRFINYMARFMYSNLDKRNLLPPLAWWLSWVTRNTIGLRVKQLGGE
                     AIRTFSLGDIVSLALHDFDNRGLRVISDVDHNGNKVTGGYRWTAAGDAHLRTIKGATT
                     KMMVVKAVKASYRELERLYAAGKEAVGQKLSSSQRADAVKKAMGLPFFAAKAFVPRED
                     LTANANVRLPGSGSGIAPLEWRWGNLGNVAYREVDITVKHGIADELFARLRDVPDTVS
                     AKGQTVRGVRHAFRSFIDRLRVDGIRVLEEAVGKKSR"
     gene            complement(7722..8387)
                     /locus_tag="PK224_02820"
     CDS             complement(7722..8387)
                     /locus_tag="PK224_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14699.1"
                     /translation="MTGIFLPSGYRPQIQADLILYLHGYKLYGYNSRMSIDSYWNKAR
                     VPYFPLREGLNDAGKNVILVAPTLGPKSQAGWLLRPNGLDRYLDQVLQALMNHGPYKG
                     SAQSPRIGNIVLACHSGGGSPMRRLALSTQRYAPHIRECWGFDCLYNGGDPDLWATWA
                     SQDPRRRLYMYYLRSTERLSKKLQAKNLPNVVVQQSSRGHNWVPINHWKERIQGANVL
                     KDL"
     gene            complement(8628..9305)
                     /locus_tag="PK224_02825"
     CDS             complement(8628..9305)
                     /locus_tag="PK224_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRB14700.1"
                     /translation="MRYALAFEAEPFEIQPKGGEFTPTPVERSGGGRITDKRAPNPTD
                     VVTVAGYQKRRVPLHRLAAEAWRALVAAARADGIAAPLLLPMSGYRDPKRQEQIWRKA
                     LVRYGSPQAARRWVAPPGTSAHQSGRAIDFYIGLAISSKNAARMRNLPAYRWLMKNAE
                     RFGFYPYEREPWHWEYNPPATREAELAAETYEEEWQGAEVYEDEAAGEAFEAWQTGSC
                     PTCGHSM"
     gene            complement(9340..9672)
                     /locus_tag="PK224_02830"
     CDS             complement(9340..9672)
                     /locus_tag="PK224_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB14701.1"
                     /translation="MLHNFGAGSAQLQPGHHATLARLADSLKRSPASDLVLTIEGHTD
                     GSPGEGSRRSLALQRALAVAKFLADRGLDIETVTRSFHGSQPVASDATAEGRQRNRRV
                     AIRICQRQ"
     gene            complement(10157..12022)
                     /locus_tag="PK224_02835"
     CDS             complement(10157..12022)
                     /locus_tag="PK224_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14702.1"
                     /translation="MVRNPKAGWSTAAHQTKVKEGTVVNLILSEPVNMNEPFLIKVTD
                     PFGNDISFEASAKTAGSETFISWDTSNLRAGRYRNIKVYKKDPSRNGQDQEIVQSNET
                     PSELDIEPRELAHRDTIAVTMRRSTPTASPDLPLWVIIKGSADALSFDNYKAHMDWVL
                     CNIPPANPNIRLNQDVQSKYQGSSDPHIQGLTQKRFLPFTDSDAYRLLKVATEAFVLT
                     NCAVLLDSERDFSEGSAEVLKRVEVSLSPSDLHTLWVDYLRTVNGNGTTTLPYLELIM
                     RKFPDIQLKNDIFIDPSVGASNTDDTDKRCYGILKQKLQQPCLLELMWSYWHEEGMLV
                     QTMAAITQRFQNRRSASGSDPLANLETDPLRPLNNLLWSYVQDEIHRLSVVRRAYEYD
                     HHYGLRIYGKAVADFRPADSRSKFLEAFHNLLNLCAKFYKQDDDTTVVADGFPLLNAL
                     RDVHLLLSEGAHNQFGDLPGTARVEMLMQQWLLARPEFRQFLPARTMVAYPEPWMDRV
                     DAMKKLQGWTDVNVLHFRNLGVFGEQVLLSIRYGAWSDTNDPKQAANWARFWRAEVQG
                     YVHAYRVVTGVDLSIDVTGKVDTTLPAIHLRNRLLSQARTPQEQNTLTIPRSRVS"
     gene            complement(12103..13386)
                     /locus_tag="PK224_02840"
     CDS             complement(12103..13386)
                     /locus_tag="PK224_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14703.1"
                     /translation="MVLQAMAAATLLHEAQGLLGRLDRVRPFALSVPMVSAAGAPQAH
                     GAIEHYVAEGRRQLRGMIQAYIRWLGGPLGRLVPPAEMNRRFVFLRMRFNALLSDFDI
                     FADVLTQRSEHGLGVLLAGLDAFAADALAIPGAFYKSPPVICYLDRGPGAAIRRARTR
                     LPGGRENPVAIIRVPRERMVGSGIASSLVHEVGHQAANMLDLVASLKIILKGLQRTGG
                     TMRLAWTLWERWISEIVADLWSVAMVGISSSLGLMGVVSLPRSFVFRVALNDPHPVPW
                     IRVKLSCAMGRALYPDPQWDSVAKVWEAFYPTTGLEAAGRRLLAILESTIPSFVDILV
                     NHRPKLLRGNSLKEALPVNERQPAQLRRLWQVWRTEAAHMRRARPALVLAVLGQAKVD
                     GLLEADRESRMVGEFLNHWALTRGLHALIPCEQRS"
     gene            complement(13389..14093)
                     /locus_tag="PK224_02845"
     CDS             complement(13389..14093)
                     /locus_tag="PK224_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14704.1"
                     /translation="MHDLDRTQMETGDSEYFEGEAFEYQETGDAYEGEAEYGEAYEMA
                     TAAASPFQEQEEMEFAAELLEVTSEGELDQFLGKLIRRASRKVGGFIKSPVGQALGGY
                     LKGAIKKALPFAGALGGFVGGPAGAALGNRLAPMAGRLLGLELEGLSQEDQEYEAARQ
                     LVRLAGAATSQAATASPAAPPQAVAQNAVATAAQGYAPGMLKGATPSGTRMMPGRRGK
                     GGRWIRRGGVIILFGV"
     gene            complement(14782..15486)
                     /locus_tag="PK224_02850"
     CDS             complement(14782..15486)
                     /locus_tag="PK224_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14705.1"
                     /translation="MASQDETDRTDTANQAGLSPQAQARSAQGDGSHGLPPPQLLSDA
                     ENKQFRLKLTIFWSVVIAAIVAAFATGYPSWENYIVQEAAKRQLRAYIDVRPRGISTI
                     EEGLVPRVHDTFHNIGRTPAYDNGSFSRILVMDYPLTRRLVNDECRHVTPDPKAGKWF
                     IGTPKRPGIARESPLTAAEVDAIKGGKSAIYFHGRVCYRDIFNEPHRTDFCLYWKWDA
                     GRISPSLSCPEGNSTG"
     assembly_gap    15613..15622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15758..17026
                     /locus_tag="PK224_02855"
     CDS             15758..17026
                     /locus_tag="PK224_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HRB14706.1"
                     /translation="MNATEQTLPTKADDYLRMRALATGLLLFMALLFVVALFQQETHP
                     MFAWLRAFAEAAMVGALADWYAVTALFRHPLGLPIPHTAIISGNTERIASNIGSLTQR
                     KLVTPEGIARLVGSWRIPEELIEALLAPERRRALTYECAQILVRALNASEDATMQRML
                     RNIATKVMRGVSVAPLAGQMLAGFLGSHQRDRLLNDILSAVLEYVDANRDSLGRTVAE
                     KLPWSRVLSFVKLDDKISHKVLDSVYDTLCTMRDDPHDPMRRKAIERLHELSQWLMHS
                     GEALRREASLKEKLLAYDTLLQFLDDSWHRLKQWMLDDLSRDPSDIRTYLDAALADIG
                     RTLQKDAALRAMLHDGVQGLVEALATRHSDKIGELVANTVREWSPAQMVETIEREVGK
                     DLQYIRINGTIVGGLVGLLLHALALLIGGR"
     gene            complement(17146..18150)
                     /locus_tag="PK224_02860"
     CDS             complement(17146..18150)
                     /locus_tag="PK224_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB14707.1"
                     /translation="MPYQPKFAVSAQVVGELERIAVLRERILSATVQVPWMPSLQRDA
                     RVRNTHSSTAIEGNPLTLEQVRLIEEGHGLPAEPSRAKREVLNYLAGLRYVERHADKK
                     RVTHRDVFTLHTTLAAEVMDQGDAGRYRDIQVYVGRYTPPEPQLVRELMAELLAWWNG
                     QSREWSPVISSAILHYQFEAIHPFADGNGRTGRALALWELYRRGFDMHHIFSVDEVYS
                     EDRPAYYAALANVRKQGDDLTQWIEYCAQALRVTLDRVWTRIQRLGASAAPKKVMLRP
                     RQEQLLQLLKDRRSMTPKEIRESLRVSKQGAMDLLNPLLEAGLVQRSGTQKAGRYTLA
                     "
     gene            complement(18366..19724)
                     /locus_tag="PK224_02865"
     CDS             complement(18366..19724)
                     /locus_tag="PK224_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRB14708.1"
                     /translation="MKPTHRWIPLPLYTQVLIAVICGGLLGALFGQEPYWGGLRNAQL
                     GKLGLFVVTLLKTLAIPLIFFAILDALIRTTIPLRQGGKLLLICLVNVSVAMAIGLAI
                     MNTWQPGLAWQGRVDALLQLVPGTKPSATVLANVQAGSQSPIEYLASYIPRTITDPFS
                     SNNIIGVVLLALVLGVTLRMVRSEKDQTTGLVNALVRGIERVYVWLVQILEWIILVVP
                     LAVFGVVAQVVGKAGIGVFSVLWIFLVAMLAGLAAHALIYYPLLAWLVGKKSPKVYLG
                     QGADAILTAVSCNSSLATVPVTLQCLHRMNVSPQSSRLAACVGTNLNNDGITLYEAMA
                     ALFLAQALGFDLPMVKQVLIVLASIIAGAGVAGIPEAGLIVLPLVLAAAGLPDHVIIA
                     AIPLIMTVDWIIARARSGVNVMSDMLVAILLDAGHVAPAAEPATIPRPSDTTHPTEPQ
                     IF"
     gene            20018..21202
                     /locus_tag="PK224_02870"
     CDS             20018..21202
                     /locus_tag="PK224_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRB14709.1"
                     /translation="MRRFRLLSLTLGLLLSCTSPALSELLALLNYESKPDQSVRREGI
                     AIMDIDPESSDFGKVLMEIPLPPDLVAHHIFFNRDRTKAYITALGKSVLHVVDLTRFP
                     YRLRAIDVPDCQVLEDLVVSEDNRIWYLTCMGSSAVIMGDAVKDKPVKTIRTPDGGGA
                     SVLYPHGIAIHNGIDRVLITSTVKPDMSDAGDSVTVLQASTGAVLSTHKVSSKPSPAK
                     AAPVEVMFAPNSNPPVVQITNMLEGTLWTGTWDAASQSFSFQQVDDYGPRQQGMPLEM
                     LYNAKGDRLFVTTAKPGFVNLYDNSDPSHPRFLQAIPAAQGAHHSVLSPDERYLFVQN
                     SLLNLEGMSDGSITVIDLSKGGTVLGSIDTLKAQGFNPNCIMLLPNHFRQANLRASLV
                     AK"
     gene            21383..21880
                     /gene="moaC"
                     /locus_tag="PK224_02875"
     CDS             21383..21880
                     /gene="moaC"
                     /locus_tag="PK224_02875"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRB14710.1"
                     /translation="MAEFTHFNESGRARMVDVGAKASTERLATAQATVFLQLETLEKI
                     QQGKIAKGDVLAVAQVAGVMGAKKTPDLIPMCHPIFLTSVDISFTETAEPNQDGRCAI
                     TITATAKTTGPTGVEMEAMTAASVAALTIYDMCKAVDRGMSFGDICLLTKSGGKSGLY
                     TRKVG"
     gene            21882..22145
                     /locus_tag="PK224_02880"
     CDS             21882..22145
                     /locus_tag="PK224_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRB14711.1"
                     /translation="MVTIQLFGMTKMLAGNQGTLSLSLHDGKRVKDLVSVIDAAYPKI
                     GELLQKKKVLVSVNQEIAHEDLEVKDGDEIALLPPFAGGSQGC"
     gene            22299..22772
                     /locus_tag="PK224_02885"
     CDS             22299..22772
                     /locus_tag="PK224_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRB14712.1"
                     /translation="MSLEQATKADQPATDDDAMLVRVQREDFSIDEELKRVRRRSKRI
                     GGIAMFLGTARDRSKGKDVDGITFEHYEGMAQKKLREIRERAIKDFGVIEVLVLHRYG
                     EITIGENIVLIIAAAEHRAEAFRACQWAIDELKQITPIWKLEHTPEGEVWVEEHP"
     gene            22826..23890
                     /gene="moaA"
                     /locus_tag="PK224_02890"
     CDS             22826..23890
                     /gene="moaA"
                     /locus_tag="PK224_02890"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRB14713.1"
                     /translation="MDHRTLDTPAPTIFDRLGRPLRSLRLSVTDRCNLRCKYCMPEDD
                     YAWLPRDTILTFEEMAELTAIFTELGVDKVRLTGGEPLLRRDLPRFVRQLSENRRITE
                     IALTSNGVLMADQAADLSFAGLNRVTISLDTLRADRFRTLTKRDLHHQVFDGIKAVVQ
                     AGFPSLKFDTVIIKGYNDDELIDLIEYGKTVGGEVRFIEYMDVGGATDWSMNQVLSRA
                     EMLEHIGRHYGEVTPIVENSVAPAERFRLPDGTSFGIIPSTTTPFCRSCDRSRLTADG
                     MWYLCLYAKDGLDLRAPLRQGRSRDEIKSLITAAWEGRADRGAEERKALEALGLREQR
                     LIEINRLREDPHLEMHARGG"
     gene            23951..24658
                     /locus_tag="PK224_02895"
     CDS             23951..24658
                     /locus_tag="PK224_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14714.1"
                     /translation="MFDPHTLTILSLGFVLGLRHALDADHLAALSTVLAERPTVQAST
                     AIGFFWGLGHTLMLLCVGTLLLALNLTIPETLASLFEFAVGVMLVVLGLSLARRIYRE
                     QWHLHTHEHDGQPHVHLHSHHLQPDHAHGHWYQGSLRPLLIGMAHGLAGSAALMLIVL
                     STVTGIGQGIGYIVVFGVGSILGMVGVGAILSLPVVYSVTVGPRAYWMVQGLACLASI
                     GLGLLMMVRIGLGGGWS"
     gene            24867..25655
                     /locus_tag="PK224_02900"
     CDS             24867..25655
                     /locus_tag="PK224_02900"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HRB14715.1"
                     /translation="MNVRVLGCHGSGQLIPGENGPIQCGTCGFLVNDQLLVDAGTIGS
                     RLFLDEQRRIRVVLLTHLHFDHIRELPTLADNLVGEIDEPVVIAAIPEVLDGLRAHIF
                     NGTVYPDFFRLPDPARPVFVPYELRAGREDLLCGMGVTPIRVNHVVPTVGFLLREGAH
                     RVLYSGDTYQTDALWQVAKEVSGLRAAFIESSFPNEMDELARVAKHLTPALLAQEFAK
                     MARPELPVYAYHLKPRFRDQIRQELGQLGIPHLTALEEGQTLVL"
     gene            25666..26502
                     /gene="accD"
                     /locus_tag="PK224_02905"
     CDS             25666..26502
                     /gene="accD"
                     /locus_tag="PK224_02905"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRB14716.1"
                     /translation="MAWFKKGKTEEAEPPKRSKVAEGMWLKCNHCREIVYRKEVDRNN
                     KVCPKCDYHFPISVIERINLLVDLGTFKEWDAELEPQDPLQFQDTRTYKDRIKAQQEK
                     TGRKDAMVIGQGAINGRKVALCVFDFGFMGGSMGSVVGEKICRAVDRALEGRMPLILV
                     TASGGARMQEGILSLMQMAKTSAAVAKLGEAKVPFISILADPTFGGVTASIAMLGDVI
                     IAEPKALIGFAGPRVIEQTIKQQLPDQFQRAEFLLDHGMIDMIVERKQLKEAVSTLVG
                     HF"
     gene            26827..28131
                     /locus_tag="PK224_02910"
     CDS             26827..28131
                     /locus_tag="PK224_02910"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRB14717.1"
                     /translation="MTTMTYSATVQFLYGLQQHGIKLGLETIRALLTRVGEPHRCYPV
                     LHIGGTNGKGSTAAMTAAILQAAGYRVGLYTSPHLIDFRERIRINGVMIPEDTVSRLV
                     ETLRGAAAPDLAPTFFEFTTALAFQYFADCQVDVAVLEVGLGGRFDATNVVDPLACAI
                     TTIGLDHEAYLGSTLEAIAFEKAGIVKPAVPVVLGRIESSTRRVIEQRASEVAAPVYV
                     VDCEFSCDGPSTADCRYVGGSSHYDHLSCPLKGRFQLDNLACALALIELARERGLAVS
                     ETAVRSGLQHVAWEGRLEMIGESPTIMVDGAHNPAAAAVLAEYLADWRRTRPAARISL
                     IVGMMRDKHPREFLAPLLSQVDTLILTQADLPRASTGSELRTLLQDCAPTAHVAATSA
                     DALAAAKRSAAPSDLICVTGSLMLVGEIKALLRGCSLSPVRG"
     gene            28133..30616
                     /gene="lptD"
                     /locus_tag="PK224_02915"
     CDS             28133..30616
                     /gene="lptD"
                     /locus_tag="PK224_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HRB14718.1"
                     /translation="MGPDRYSASIRRRTAGCPFPVVLLTLLLSVLLLVEAASAQSTVT
                     TASPSNQPLTVTAERIDHLQEQEVYEADGSVVVTQGSFRLTADHVTINSLPGTLVATG
                     HVHFFDPSSDLKSERLELNVNTEAGVITHGSMYVRPSNSFFTGRSIRRLSEDHYRIKE
                     GTFTNCDAKDGEVPAWRFKFDDLDVNTGESIGMKRAWLCMRDTPIIPVPTLTYPLSNR
                     HSGFLIPTPGYDNRFGLHYQQGYFWAINPSQDLTIAPSYYSDLGYGSDFTYRYYLDRR
                     SSGQWFASFLQQTKLPNVSGVDQTASDERRLRGLISGQHVQQVTDTLLVRGQASFVSD
                     RQYLQQLSNSGAQRASPSGESTLLATQRLPYGSAYFLGQYLQPLNSGGPDTFQRLPEV
                     GYVLPNTSAFGLPLLMNLDSNFVNFYREQGFAVNRMDFMPGVTTDVIDVGHVIGLTPH
                     FKFKEVYYTRGIQEASPLHRETFWAAMDASSRLSRRYTGGDGGSFLHTIEPSVMYEYV
                     PGSNQSQIAQIDQVDDIPKKNLLTYSLRTKLLEQQVNGQSFNWLDLTLAQSYHVGGVQ
                     TRAREFTPGVLPFLGSLTQPLQPATVEVQGRKLSDLWLRAVIGNTAPEFFRPPGVNEA
                     LGKNVTGGQMLPPTNSYLTVDAFLDPYRGTFSQWNTDLRVQQSNYWYVEVGQRFSRDG
                     NRVRRGDIWNPVSFNEVYAPTEEIQFVTAGGGFRTPWGWTVGAKGYYDVKGGRSPEYD
                     VVALYQNPCKCWSLGLYYLQFPDRQSYSFMLSMTGIGWTENFGTIVARTILGPLLVGD
                     RGLPWAAPGGPYGRTLNPMPVQSMPMGRY"
     gene            30695..31228
                     /locus_tag="PK224_02920"
     CDS             30695..31228
                     /locus_tag="PK224_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14719.1"
                     /translation="MRRNQLFLLIGVCLWSAVQGAVPAQLEAAEQPSHLQRAAVFLNA
                     ADYRQAVEACQAAVRETPSARTYVFLTYVYHALDGYLEHLATTDQWVRVEQLYLNLAT
                     GRSEDLIDPPDVLARIAKEVIQQAVQRQSDVTAAMAARLDEALVKQFWQEQDAWRRAR
                     PTDWWSGVPPEWHWDRP"
     gene            31333..31680
                     /gene="trxA"
                     /locus_tag="PK224_02925"
     CDS             31333..31680
                     /gene="trxA"
                     /locus_tag="PK224_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRB14720.1"
                     /translation="MAGDALKATDATWDEDVMKASELVMVDFWAVWCGPCQMVAPIVD
                     ELATEYAGKVKVRKLNTDENPEIAGRYQVMSIPTILFFKNGQAVERLVGARPKRQFKE
                     IIDSLLAQHSGAA"
     gene            31699..33396
                     /gene="recN"
                     /locus_tag="PK224_02930"
     CDS             31699..33396
                     /gene="recN"
                     /locus_tag="PK224_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRB14721.1"
                     /translation="MLTELRISNFGVIEQLAVRFGSGFIVFTGETGAGKSLLIDAVTL
                     LVGGRASTDKIRAQSDEADLEAAFVLPSDHPLLHLLQTKEFVRPGETDIVIRRVISRT
                     GRNRTYLNGNLCPVHLLEELGGALVDVHGQHEQQSLLSSAAQLEALDAFGRLHALRHD
                     YQVAYRSWQERVAERETLTVHIAQRREREDLLRFQFQEISDAAVEAGEDARLEQERPR
                     LMHSQQLGDLSDQLHELLYAGDQGVLSLLASARKLLAKMVSIDQTAVEWTRVVEDAIV
                     PLRDLADQIRHYRDQVEANPARLMEIEQRLDRLHRLSRKYGGSLDAMLVLQDSLRAQL
                     AQLDTAETRLQDLATAVEDAARRVGEVAGRLSRKRIDAAKKLTTQVTKELSALRMERT
                     RFAVEVLPLPGETSYGQMGHDAVEFVFSANPGEPLKPLAKVASGGELSRVMLALKTIL
                     AESDRVPVLIFDEVDAGVGGAVAEVMGTRLRDLSRHHQVLCVTHLPQVGSQAHAHYLV
                     EKQVRQQRTVTQVRLLTEREREEEVARMLAGVTVTNSARAAAAEMIGSAKERQARSD"
     gene            complement(33393..34535)
                     /locus_tag="PK224_02935"
     CDS             complement(33393..34535)
                     /locus_tag="PK224_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB14722.1"
                     /translation="MGTHTSPEYVGTKPSVLVVDDETGPRDALKVILRPFFTIHSADN
                     AKSALRVLKDQHIDLITLDQKLPDRQGIDLLQDIKQDYADIEVIIITGYGSLKSAMEG
                     IRHGAAGYLLKPFNVTELITLINQTLEKKQRLDYLRSFLKISTALWGTEQEAAQAWKD
                     LQANYFSIGKSAPEPAAKTGDVTALIPLLSDLLEAKDRQLLNHCSRVSFYASLLANQL
                     NLSLPDQKGLALGAFLHDIGKISIPNYKYAANDDDSIKNGTLAKAYSEAGARMLLPLE
                     FQAEVGQIVAYHHERFDGLGNPHGLEGEGIPLLARIVAIAQAFDNLTVDMPGHLPMSI
                     DDAIRKIVLQADTHFDPKLTELFAQVVRECKSSLPALAIAKTSPTT"
     gene            complement(34724..36136)
                     /locus_tag="PK224_02940"
     CDS             complement(34724..36136)
                     /locus_tag="PK224_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB14723.1"
                     /translation="MKKRVLLVDDEPRVRASLRTVLEPTYEILEAADAAEGLKSFKHD
                     APDLVLLDVILPGTDGLAALQTMRTENRAVPVIMLTGTKSVKTAVDAMKLGAADYLSK
                     PFDVEELQIVIERTLGKQELEQEVRQLRAQVVQRYAFHNLIGKSPAMQEIYAKIEQVA
                     DSRTTVLVTGESGTGKELVAKAIHYNSARRERPFVALNCAALPETLIESELFGHEKGS
                     FTDATARRVGQFELAHTGTLFLDEIGDLSAATQAKLLRVLQEREFTRVGGVQSIKVDV
                     RIVAATNKNLDEMVRKNQFREDLYYRINVIALYLPPLRERGEDIALLAKHFLAKRIEE
                     EKRPPQEFTKSSLELISHYPWPGNVREMENIIEQAFIWSKGSDVITPEHLPNILRTDT
                     RSTSLRDDTLAGRLSLEKAVMEFEREIILDALKRTTYVQTHAAAMLGISRRMLKYRMD
                     TLGISRPDNGVNPEPPLTQE"
     gene            complement(36138..37355)
                     /locus_tag="PK224_02945"
     CDS             complement(36138..37355)
                     /locus_tag="PK224_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB14724.1"
                     /translation="MIGAFAESVGQATDLTQVGECILRILSEQLRLPKAAIWIREANG
                     EYHLLASLGHRTLAALPASLPDNHALITGLSDRLHLLRYDQASSSPVGSAMISIQAAV
                     CLPLRNKTQFLGLCALGPIGTECHLDEDTVIIAETVARIACNTLDHHLAQDDLRRSST
                     LMRRTDRLRSLEIMAGGFAHEIRNPLTSIKTFIQLAPQRQQDPVFIREFSQVAIEDVH
                     RIERLLHEILDYASYMTPQPSDVDLNELVTSCLGFVSSTASHRGIRLHTTLAPQLPTL
                     SLDRQQIKQVLLNLLLNALDAMPNGNGVIQIRTRVLPQAGRTAWAQLEVEDEGCGIAP
                     DHLEHIFDPFFTTKHTNSASDGSGLGLTTAHQIVREHGGTLLVESRVGAGSTFSVNLP
                     APDARTTVSAARE"
     gene            complement(37525..41730)
                     /locus_tag="PK224_02950"
     CDS             complement(37525..41730)
                     /locus_tag="PK224_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB14725.1"
                     /translation="MKSSMSPAEPSLNILLINEQPDEIKLVTSSLRGFFPGCRIEAGY
                     SSEEALTFSHQGEWHIILIDHDLRPERGLDILSRLRRNAPYAAIILQTNESDSQTAVQ
                     ALQNGADFLLFKRSPAFITELLFSVQEAVEKRDLQMKLDHTFQRHQRFIETLSDLVYE
                     LDRDGRFVYVGTAVTAMLGYTPEELAGRHYSLLLPPLQEATGRFRLNERRAGSRSVRR
                     LELTLHRKALPDAPPASITVDVTAKGLFDSANRYLGTVGVLRDLSQQKAQQDRLTELE
                     SRLQETTRQLTLSREAARVSRQLQQPLTTLLQDSQRLLSSIQHSKIEQHVETMIEKAS
                     QASQLGHQLAQAIHAPPLEFFPLDLHHVLETVVQTIRHETEGSGRLVTTHLAQHLPLI
                     LGSPDALEDLVRILLNYAHQYTSLPNAPSHLLLQTALLAGVTPSLASRNLPLYSSVSR
                     EYVSFTIKESSKHTAAPMGEPPDFHISPEQFFRAHQIVQAHGGAIEIEHAPDSGLTIT
                     VRIPVPTHFFADEGAESPTASPAKPGIVRVQSQHVAGERPDRRRSERKLFALPVQLSV
                     GNTTLRGVLRNMSTGGALLTIHDLSASIHLQPAYVVIKTPVSFLELQGVVHERPAALT
                     DLPLPAIKNFVISFSPSGERDRNVLRSLLDGLQDGSTIVTFEGLILPLFTTAANNPDA
                     PLPSTAAPTDRREAARLNVPCPIRLVGSSATPTRPLGVTVNLSLDGACIELPGGSDFP
                     ENQQILHLAPVEPNTSSIGAALPEGSENPWPARIVWTHQSQVRTTSNPAPPEGVRRRF
                     GVRFQGLSPAQEQRLRALISPRIGTSQDLAEPMSDSPVLTVSHHLRNREGLTIALCHD
                     VPRRVDGTQCPLVLLCSGYGTTQQAYVALAYTLAACGLQVLRYDHSRHIGLSDGDPTQ
                     TTLTSLEDDLDTILIFAQKEFPGLPLTLLAPDLLGRIALRRQDWHRSVRRLLLLNPTL
                     DIRNCLTSLHQRDLVGEHLAGTRLGLGNLLGIPLDIDHFVADAVAAQYTESNALQEDL
                     SHCGTEVVFLTTGPGAAEYPTPSPAPALVEDALHRLGSRGSRVMLQAPLLPADAVAPS
                     ALHASWHRIAQLCLTPDSFPYPTAPAPSNLSRMISIRARLERDRLRIKYAAGTAGRER
                     LWAAQTDLTRSLDELPAHWQYIDQLYQLLQPLDGGLALLDVGCGIHSFARLLLLNLSY
                     RLRAQTWNQNQPLRYIGIDFSVAALHAAQDATSDALKHVDRLFSGRISGPTPVSQQWV
                     RGRSVETLPFADHSFDRIVANLSLSFSRSPLHALRELFRVLRPGGKLIVSVITPLADM
                     ALPYRSSLQELGVDAFSGDARLALNRMAQCCVALRVGQLHAFQEESLSAALAQVTSTP
                     ARLVRTLSGQILVALAEKPDSAG"
     gene            complement(42121..43122)
                     /locus_tag="PK224_02955"
     CDS             complement(42121..43122)
                     /locus_tag="PK224_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14726.1"
                     /translation="MNRALLSMVMLLMVTSTVYADGGHDHHAIPTMPSLGDTKITIDD
                     HTVTLTFGPVDLPTGHDGDLASSMPKKVFQLPDDTYMIGYKSEVYTKDGKPLPKNYLH
                     HILMLNNDKPSVSCDGEPLFFAGAGLEMTEARFPDGYGVKLGKGQNLMSVVAFYHKAP
                     PTKDVMARFTMYVAPKGTKVQEMDVYQVGVNIVCYSKFGQRGADQTDEGIEIKPGVSV
                     LSAPLKFNMDGCVKFAYPHGHDELLMVALENKSSRETLLRTVPEVGADGTFRDFRPHQ
                     VYRDDKGFTVTKRDDYEMVMVHHHPLQNPNIQHGMGNYLLYMTPGVCPQSANTASAR"
     gene            complement(43196..44035)
                     /locus_tag="PK224_02960"
     CDS             complement(43196..44035)
                     /locus_tag="PK224_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRB14727.1"
                     /translation="MSILAGSLAAAKEPLDAEATLRLIVQANADRDLPTMSKYMSHDA
                     DAVGYTIDGHKYVGWTALATDMHTEFTSVAKLELPITYLQFWRKGDIAWFAMELDYIR
                     YLNATDLTQRMVLPLRETGVMERRDGTWILVTWHESTRQPDTGMGTALADPGTLVRRV
                     SDNQAPGPNGADLSGEWEVTEIEDNKKYVATLDAHGNGPYTWQGGQFATTSFNDRRWQ
                     GTWKQTGNDREGAFEVVISEDGTEAKGIWWYVRVGTRDNIPPRQHGGTYLWKRLTPPP
                     TAP"
     gene            complement(44202..45008)
                     /locus_tag="PK224_02965"
     CDS             complement(44202..45008)
                     /locus_tag="PK224_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRB14728.1"
                     /translation="MSTQQNRWAWRAFGQTERGLVRPTNQDTFLVENRHRLWAVADGM
                     GGQAGGDVASRLVMDSLAELAPTLGGIASAPDLHEEERATDRLTTMAEQAHASILTHA
                     AQHPPLYGMGTTLVMAHLLPLPKPRLLVLNVGDSRAYLVRAGTLTQLTRDHTLIEEYV
                     RDGRLTPTQAACHPNRHVLTRAMGLAPHLESDLFFFDLLDGDLLLLCSDGLTKMLTDE
                     RILQTVRPHRHIPSLATQALIHTALDAGGIDNVTVVACTITEIPDQANIH"
     gene            complement(45010..45783)
                     /locus_tag="PK224_02970"
     CDS             complement(45010..45783)
                     /locus_tag="PK224_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRB14729.1"
                     /translation="MLTSTFVHLNGIGPSTERRLWEGGIADWTTFRREPNLPGISPSR
                     KVLYDIDLASAQEQLDRRNARYFAGCLHTRDHWRLFRTFGPRALYLDIETTGLSAHEG
                     QVTIVGLYRQGRLRTLISGDDLTLGVIQEELDQADLLITFFGSVFDIPYLQTCFKGLR
                     VSIPHFDLCFAARRVGLQGGLKHIERELDIARDSDLLTLDGMEAVRLWHQYRAGDEAA
                     LDQLVRYNAADTRNLEPLAEWLYDRLALRYGPPSLTPAG"
     gene            45916..46326
                     /locus_tag="PK224_02975"
     CDS             45916..46326
                     /locus_tag="PK224_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HRB14730.1"
                     /translation="MTTNGSAKRIRIKVGGIQLEAELKSTRTAEELYQALPAEGPLNV
                     WGEEFYFKIPGVKDHRETATTQVKVGDVAFWGAGQVLAIFFGRTPMSMGADPVPADRV
                     NVVGRVVGDAATLRQAMNASTIRVERIEQGASIG"
     gene            46384..47013
                     /locus_tag="PK224_02980"
                     /pseudo
     CDS             46384..47013
                     /locus_tag="PK224_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
     gene            47049..47489
                     /locus_tag="PK224_02985"
     CDS             47049..47489
                     /locus_tag="PK224_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB14731.1"
                     /translation="MRVALWIVFCVMAVSLPACTHSKPQPLVPLELAYGAKADAIALN
                     NQGMQAYLAGQVAEAKNFFGQAIKASPDSGQAHYNYALALNALGQTDQARLEFIEAAN
                     LAPGDKVIWDSPALRPFGSPQAQKGPPREHPYGTQRPVIGSGPR"
     gene            47529..47996
                     /gene="bcp"
                     /locus_tag="PK224_02990"
     CDS             47529..47996
                     /gene="bcp"
                     /locus_tag="PK224_02990"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRB14732.1"
                     /translation="MADELDVGAKAPDFSLPDQDGSTVTLKSLKGKQVVLYFYPKDDT
                     SGCTKEACDFRDSLAPIKKAGAVVIGVSKDGKASHQKFIAKYGLPFALLSDEEAEVCK
                     AYGVYKEKSMYGRKYLGIERSTFVIDATGRIKALFRKVKVPGHVDEVLAALKA"
     gene            48353..48595
                     /locus_tag="PK224_02995"
     CDS             48353..48595
                     /locus_tag="PK224_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14733.1"
                     /translation="MDPLEKTSHACRAAALSLVPGLGHLYIGEKRGGPLLLAGVLLIV
                     LWMAVFSFVLVPLIGLVLFCMGDTYLTVSRDRGLLT"
     gene            48636..49484
                     /locus_tag="PK224_03000"
     CDS             48636..49484
                     /locus_tag="PK224_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14734.1"
                     /translation="MAEWLRLGSRTFLLLLLFGWTMAGTGEAAEKVQGILTVHDALTL
                     PNHPARIEAQLGGKAPGGSSLWGGVGLHLSIDGKPVAMAKTNESGHASFDYLAKMRGT
                     YAVTVSVDDAASVAADNSDATLCVWERRRPIILVELAALIQPVTSASTGTAAESSLQP
                     LSDAADELSRLTQYYYNVVYVTGDEQPPSEIAAIGNVRRWLESHKFPPGFVAPPSEGL
                     GATLETFKQGGWATMKTGIVRTRSSAEMLLQHRFEVVVVPELPKGEMPRKAKAAKEWK
                     DVRKKL"
     gene            49671..50450
                     /locus_tag="PK224_03005"
     CDS             49671..50450
                     /locus_tag="PK224_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRB14735.1"
                     /translation="MLLEGKKGLIIGVANKHSIAWAIAQAAAREGAQMLFSYQNERLR
                     ENVEELVQTLPGASACVCDVGDDNQIDAMMKQAAEKLGRLDFLVHSLAFAPREELTGE
                     FVNTTRQGFATALDVSAYSLVAVTRAAMPLMTEGGSVVTLTYLGSERVVPHYNVMGVA
                     KAALEATVRYLAHDLGPKNIRVNAVSAGPIKTLAARGVSGISKMVDHHRAFAPLRRAT
                     EQGEVGDTALFLVSPLGRGITGEVIYVDGGYHILGSLASVD"
     gene            50512..51411
                     /locus_tag="PK224_03010"
     CDS             50512..51411
                     /locus_tag="PK224_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YD repeat-containing protein"
                     /protein_id="HRB14736.1"
                     /translation="MTRHIREVSGSSFFLTLFIVLLTVGPVIPSFSAELSDREEDGLL
                     GAVQSVEIKESLLVQIDRYDPRGRLIERIQGGGEISQGLWPLRFVYTYDQAGRRTAEV
                     VQDARGAVVKETRSVYDDRGNRSAELAAWGDGTFENVSLYEYDEAHRPIRGLHFNGVQ
                     VINRNLYAFDSAGRLVRERFERNYHYNAAGTQVVMTGRFDTGYEVAIVYDERGQVREK
                     IVSDLRSRPQGRSEFRYDEQGNQNEERIFNATGQATDRKVYHYEYDVVGNWIKEALQW
                     WDMADGRERLKQSHVRERSITYH"
     gene            51575..53041
                     /locus_tag="PK224_03015"
     CDS             51575..53041
                     /locus_tag="PK224_03015"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HRB14737.1"
                     /translation="MNRLRLLSSIVVMMLACAVGERGAWAGDDLAPLLTRGPLRVSND
                     NPRYFADSENRIVYLTGSHSWENFQDITDAFDYQAYLDRLAALNHNFIRLWVSESIQS
                     DGGWLMHPPARKNPVVPLPYLRTGSGVALDGGPRLDVSQLNPAYFERLRARVQQAGQR
                     GVYVSVMLFQGFSVVERGAWKAHPLNGHNNVNGIDGDANGDGVGKEVHRSPTSQVLNV
                     QEAYVRKVLDTLHDLDNVLYEVVNEAIPESVPWQYHFIRFIKQYERDRQYAAHPVGMT
                     FFQLGEFGGGDNATLFDSPADWISPGGYTKYARNPPVADGPKVMLLDTDHIHGIGGDQ
                     DFVWESFFRGYNPIYMDPLGALHELTIGEPVLNEPQHERARMAMGEARRWAERLDLKR
                     TLPHEELASTGYCLADPGPAYLVYVPASASSHSTPDVSRPLTVNLTGAVGGFVGEWID
                     LEQSSAMPVSERIEGGGERLLTVPFRGAGLLYVYRQKD"
     gene            complement(53091..53243)
                     /locus_tag="PK224_03020"
     CDS             complement(53091..53243)
                     /locus_tag="PK224_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14738.1"
                     /translation="MNKEEFRRCNFTVGQAVSFAVTHFRILPQEGSTLSHEAKPGAPI
                     TRPLTP"
     gene            53494..55836
                     /locus_tag="PK224_03025"
     CDS             53494..55836
                     /locus_tag="PK224_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRB14739.1"
                     /translation="MGRCERMFRAVGVVLALAFTLVTSLPLHAQTVLFGPTQYTRTAG
                     LPNQFTATFALSAGTTAPYTIHIVNGNADGTKRISSATIKVNGLQVAGPGDFGQNVAV
                     IDRTVSLQPTNTLEIRLTSAPGSLLTISLLDTSAGSQPTFLAPNPLNLLAGASGTVSA
                     ALAPTPTAAGTLAVSSANVAVATVPTSIAFMAGQSSVPISVTAVAAGSTTITAMLNGG
                     SAASQVTVSPAVPTITGFTPTSGQPGTSVTVTGANFVNVQTVTFNGVSSTFTVTSPTT
                     LIAVVPPTATTGSLTVQTLAGTATSAASFTVIPAPTLTSLQPATLSIAQGSTGTLTVT
                     LNAVQPADTIVTMASSTPTVATVPGSITVPATQLSAPIAVTGVALGQADITASLNGTS
                     ATSAVTVILGSPSIIGSNPDSGPAGTVVTIAGSSFNPTAASNQIAFNGHPAIVSSVNT
                     AGTALVTTVPQGATSGPVTVTTLVSPPATGPVFTVTVPDFTVAALPTSLLLPVTGQGT
                     VAVTVAGSNNFSGLVSLSTLGVPTGVSAVFASTTLAAGQSGMLNVTTTGATTAGTYPV
                     TVQATGLVNGSPIDKTVTVNLEVLSGGLTSFAGQVQDEDNQPVKGTRVTMGAVHAITD
                     AGGNFLLQNPPVGVDQLFLIDGGPASTPGRNLPIIPYKGTIVAGQANVLSFIPKLHVQ
                     KTTGLVDIADSSVQRVVTDLELPGYQMTIPAGATITGWDGQPNTQVSIRRVPLDQNPL
                     PPIPGDRVATDIYMDYFGKAGGGTASAPLRNRFPSRCRMI"
     gene            55956..59453
                     /locus_tag="PK224_03030"
     CDS             55956..59453
                     /locus_tag="PK224_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRB14740.1"
                     /translation="MPDIDPATGKAYGQPRFCCGGIFAAMLRAAFDLINNQVNGQPQT
                     PNGAKGGEPVDLGTGAFNLEKTDMVLPGRLPITITRHYRSNGPATGPFGPGTSHGYQV
                     VVRAEANVRTLFMPDGSRMQFRQQPDGTFRNSSEPTVRGAVYSEVNGRVLRFKDGTTW
                     TFGAGSQGFFFLTALADRNGNQLTLTRSGATLNVTAVTAQDGRQLTLAYDAGNRITQI
                     TDPIGRVVQYQYGGSGRLVSLVDPEGGITRYVYDAAGRLVTLTDARALTFLTNEYSPG
                     SSRVLRQTQVDGGIWKFRYQLSGARVTGPGCPAPGSQDLWVVIEGTPICPREESAEKV
                     AQGYQFVGGVVTAATVLDPLDHTTTYRFNGAGHSVSQTDALGQTTISTRDGNSNQIVS
                     SIDPLGRTTRFEYDAAGNVITITDPNNQATRFEYYPTWNRVTKITDALNQVTALGYDL
                     ANGNLLTVTDPLNHVTTIAYNSFGQPTSVQGPIPSEPPTTFAYDTNGNLITTTDPLGH
                     STQRVYDAVSRLTALTDPRGLITQFRYDGLNRVTEIADARQGLTHFTYDPNGNLLTVT
                     DAKNQTTAYTYDTMDRLKTRADGLNRTETYAYDPAGNLASFTDRKGQQTTFQYDALNR
                     RIQATYPDATTRFTYDAVGRLIHAADTAPGAGALDFRYDSLDRLIQEITGQGAVAYQY
                     DGLGRRTSLVANGLVPVTYGYDAASRLTQVAQGVLAVGLGYDNANRRTSLTYPNGTST
                     SYAYDVASRLTNMTHNGPSGLIEGLAYTYDSAGNRTSLARANATASLLPNAVASATYD
                     AANEQIAFAGATLTYDQNGNLTNDGVNMYIWDMRNRLIGLSGGAMASFQYDPIGRRTN
                     KIINSVATQFLYDGVDIIAEIGGGAVGANYLRSLIIDEPLIRQTSEGNEQYHMDALGS
                     SLALSSSQGNSVTAYAYEAFGKTTVIGSSSNSFQFTGRENDFPGLSFYRSRYYSENRG
                     RFLSSDRVGLAEGLNVYAYAANNPVRFSDPWGLWSAEGHNKFLTDRFGGGMDPELLAQ
                     VLAGSASVDAPAMQTPRFAPMHAMTSDVFPTEKVARKAMCDFVNNNMNAFRNYLNNNE
                     PAIAYHHLGQALHPVMDTTSPAHRGFQYWRLPHSKAELDEHIAQETLDTITPDLERQT
                     LSLMNATLSGDYSVLGCGK"
     gene            59501..59977
                     /locus_tag="PK224_03035"
     CDS             59501..59977
                     /locus_tag="PK224_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14741.1"
                     /translation="MHVRKGGADSISIDIIFVGTLFFCMIPADAQASGMAIFYIAYWV
                     VGFSQLLPLLLLCSRRMQGRCVPASLAYLGIVATLWNYVVLALPQDLVLKFSEAEWFS
                     RTIYRKREYLLEKFGPDYGKTLFDVLTFYVPLLLPPMLAWGIYLLLRRKVSHTPVQ"
     gene            60073..60318
                     /locus_tag="PK224_03040"
     CDS             60073..60318
                     /locus_tag="PK224_03040"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HRB14742.1"
                     /translation="MSIEVCVGATDSVTTITDALNQVTAFGYDLANGNLLTVTDPLNH
                     VTTIAYNSFGQPVSVQGPISLNRPRRLPMTPMAISSR"
     gene            60315..60461
                     /locus_tag="PK224_03045"
     CDS             60315..60461
                     /locus_tag="PK224_03045"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14743.1"
                     /translation="MTDAKNQITTYTYDNMARLKMRADALNRGEIYGYDFSNVAATFE
                     PKGC"
     gene            60487..61260
                     /locus_tag="PK224_03050"
     CDS             60487..61260
                     /locus_tag="PK224_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRB14744.1"
                     /translation="MVPHLLISLVLVAALIVPVSLPADTAQYFYDELGRLVGVVDGQG
                     NTATYVYDEVGNLLSIQRFSTSGTGIGLFVVAPGSARVGTTIMLQGFGFTSPPASNQV
                     QFNGISATVLSSTATTLVTTVPLGAATGQVTVTNANGSATSPQPFTVFVPPIISGVQP
                     STVPKGTTTQLVIAGFNLDGASSVTFAQSGLSATVLPSSTAQSLVVNLAVAGTVPDNT
                     YSFSVTTPAGTTQSGTVVARVAPAMTTFHVAKPVSVFKP"
     gene            61313..62755
                     /locus_tag="PK224_03055"
     CDS             61313..62755
                     /locus_tag="PK224_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14745.1"
                     /translation="MRRWSVCVAVLLLSNWTTVGWAQTFLSGSTGADGPFQPTANTTL
                     PVPTSGIFHFTTVTIPTGVTVTFTPNATNTPVAILATGDVSLAAGSIVNVSGQPGTPG
                     SSTGPLVNPGGSGGPGGFRGGQGGVRAPTGPVTFPSAGQGPGGGGLPTYPPPQFGGCG
                     VGGTYGTPSTFTSLLSFFGGSGGGGQNGYPGSTSVSGSSGGGGGGAILIASSTRITVA
                     GAIQANGGRGGTASNLTVLTAGSGSGGAIRLVAPEIAGSGSLVARSEAIGCEAGSPGV
                     IRLETSRRLFSGTTNPVASVSTTMSPVAPGSTPPLVNLPTLAISSVGGAGVPANPVGL
                     FGVADVTIPLEITNPVTVTVVATNIPVGTTFQVKLIPQAAAPTTLTSSASVGTFGLST
                     ATATLNLPPGQVSVLHVFGSVALPQVAGLVPVIDGEPAERVFVAGGLNEPSTTMVVTK
                     SGKEAPLSQLSAEDQVKVARAFDAMRISAP"
     gene            complement(63062..64159)
                     /locus_tag="PK224_03060"
     CDS             complement(63062..64159)
                     /locus_tag="PK224_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB14746.1"
                     /translation="MKIEVRGLTKKFGSGTAVGNVSFVVNEGELLGLLGPSGSGKTTV
                     LRLIAGLEVPTAGDIFIDGKRVNDLTVQERNIGFVFQHYALFKHLTVFDNIAFGLKIK
                     KWNRRDIEQRVQELLALMSLEGLGGRYPHQLSGGQRQRVAIARALAPRPSVLLMDEPF
                     GAVDAKVRQELREWLIRLHTDLNVTSLFVTHDQEEAMEVSGRIIVFSKGKLEQAGSPA
                     DVYEEPATEFVARFIGSMNIVEGVVRRGQVQVGSLEFPAAGFSDGQKLQVGFRPYYVK
                     VAEDPSRYRQQAKLRHIYFLGVAYRLEIETPDGLILRSRMNKEEFRRCHFTVGQAVSF
                     AVTHFRILPQEGMAPLDGTENSRPLTNPLTP"
     gene            complement(64164..64973)
                     /locus_tag="PK224_03065"
     CDS             complement(64164..64973)
                     /locus_tag="PK224_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HRB14747.1"
                     /translation="MRWLLIGIVWLYFLVLLVGPILYMASQSFSEGLAAFWAEISRPE
                     AMHGFTLTAEITLIVLVLNLIFGTMTALVLARQKFWGRSLVSGVIDLPFAVSPVIAGF
                     MLILLFGPDTVLGTFFGQAQIKVLFALPAMILATLFVTFPFMVRELTPLLQTLGTEEE
                     EAARTLGAGEWQVFLKVTLPALRWGLVYGATLTVARAIGEFGAVLVVSGNILLLTQTA
                     TLHIYQSYVDFNYVAANAVALTLLAVSFAILTVLEIAKARGETTVAEAGAQ"
     gene            complement(65022..65903)
                     /gene="cysT"
                     /locus_tag="PK224_03070"
     CDS             complement(65022..65903)
                     /gene="cysT"
                     /locus_tag="PK224_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRB14748.1"
                     /translation="MTAHLLLSLALRSAAVGYVLVLIFLPLAALAHQSFAAGWDRFIQ
                     DLSAPQAAAALWLTVETAAIATAINALFGTLSALVLVRYEFPGRWLLNALVDLPFAIP
                     TLVAGLMIAAIYGPTSLLGTWLQQGGMAVLYNQPGIILAMVFVTMPFTIRSLQPVLMG
                     LERDQEEAAFTLGASPWITFWKVTVPSILPGLLTGVFLTFVRALGEFGSIVIVAGNIP
                     MKTQVASVYVYGEIESYNPQAATSVSVLILLISFLVLLLLERLTRRPGERLPRLFQWT
                     RQKDERGSESAVSTATI"
     gene            complement(66006..67061)
                     /locus_tag="PK224_03075"
     CDS             complement(66006..67061)
                     /locus_tag="PK224_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB14749.1"
                     /translation="MTRQTRTIFGLALGAWLTMLLPTTAPVQAAETRDLILAAYSVPK
                     EAYERHIIPAFQRHWKQKTGQDVRVRSSYGASGAQARAIIGGFDADVAVLSLEGDVDQ
                     IVKAGLITHDWRKAPHGGMISASVVALGVRKGNPKGVKGWDDAARPGVEVLYPNPKTS
                     GGAMWDVIAIYGAGLKLAEQRAGGKPLAPEAAASQAVDLLTRVQRNVKVMDKSGRESV
                     TTFERGVGDVIVTYENELLPRVKSGRPYEIIVPAETVWIENPAAVVDTYADRHKARDL
                     AEAFVAFLHGPEAQAAFVELGFRPLDKNAGTQATTLPLPARLFTIADLGGWDQVSNKL
                     FGAQGLWTKIVEDVTKK"
     gene            complement(67084..67509)
                     /locus_tag="PK224_03080"
     CDS             complement(67084..67509)
                     /locus_tag="PK224_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRB14750.1"
                     /translation="MKFSKKSEYGLRALLELCETYGGRVLQRHEIAERQNIPVEFLEQ
                     ILLALKRAGLLASRRGVRGGYSLIKSPEEITLGQVIRILDGPLAPISCVSKTAYQKCA
                     DCPYAAKPSCPLQQAMGEVRDAIANILDHYTLSRFAHSK"
     gene            67752..68672
                     /locus_tag="PK224_03085"
     CDS             67752..68672
                     /locus_tag="PK224_03085"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRB14751.1"
                     /translation="MNFQDLILTLHRFWADRGCVVHQPYDLEMGAGTFHPATFLRSLG
                     PEPWRAAYPQACRRPTDGRYGENPNRMQHYYQYQVVLKPAPDNIQSLYLESLKQLGID
                     PKKHDIRFVQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGIPLNPITGEITYG
                     TERIAMYLQQVDNVYDLAWTDGVSYGDVHHRSEVEFSRYNFEEGEVPMLMATFQAFEG
                     ECQRLLDKKLTLPAYDYCIKTSHMFNLLDARGAISVTERTSYIARVRALARRCAESYL
                     ADREAMGHPLIKQSAGTGKRAGAHSPVPTK"
     gene            68693..70885
                     /gene="glyS"
                     /locus_tag="PK224_03090"
     CDS             68693..70885
                     /gene="glyS"
                     /locus_tag="PK224_03090"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRB14752.1"
                     /translation="MPTKKTMTKTASRSKVAKAPKAPATTELLLEIGTEELPYQFVAP
                     AMRALQQSAETLLKDLRLTYGTVRTMGTPRRLVLLIEGLARQQASAVKEAMGPSKAVA
                     FDQNGQPTRAAVGFAAGHGIPVEDLQVRQTPKGEYLFAVKQEKGQAVAAVLTQALPQL
                     LGKLSFPKAMHWNQTGVRFARPVRWLVALCGGKVLPIQFATIKAGNLSHGHRVLGAKV
                     SSPKGFAVKSIAHYVKETERHGVIVDQERRRAMILDQLASLAKSARGHLHQDDDLLEQ
                     AVYMVESPLTILGSFKPHYLSLPKEILMTSMKEHQGYFSLVDQKGALLPNFLAVTNMK
                     LSNMQLIREGNERVLAARLADAKFFFDEDRKTSLVDRVAKQLAVTFHQKLGSLHQKTQ
                     RVVAMAAHVAGQLGDERLVQDCRRAAELSKADLLTGIVGEFPTLQGLMGGEYAKHDGE
                     SPVVSAAIREQYMPRAMEGELPESLAGKVLSLADRLDSIAGFFLVGMVPSGSEDPFAL
                     RRHATAIVRIVIESKLRLNLAQAVRAAQDVLSGQNVVAAPQSGKGGAPDVIGFLFERV
                     RFYGKSAHQVRDDVMEAVLKSADRQTLDLTDLFDKMKALQQITVRAEFDPLIVGFKRA
                     HRLTEKEQWDRKPVESGLFREGAESALHQTVQSSQQDYAAAMHGGQYGQALDVLVRMK
                     GPIDDFFNAVMVNADDPAVRGNRLSLLKSVDDLFMSFADFSQIMVQGT"
     gene            70900..71418
                     /locus_tag="PK224_03095"
     CDS             70900..71418
                     /locus_tag="PK224_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14753.1"
                     /translation="MDSTSESLPESPEASAAAQLRATKVRRFASGISVVMMVVCNAVF
                     LFGIWVTGVNFERLVRTPDIYDSRQDICLRLAYQHVPGSENPVQFCSEWLNLADPTGK
                     THTFQKDAQLKQGADGKFYFDYGPFVDYRLFAVGAFVVGIIVCGIRVTRHVVNRYRLR
                     LEAAARHSVPTH"
     gene            71444..74293
                     /gene="ppdK"
                     /locus_tag="PK224_03100"
     CDS             71444..74293
                     /gene="ppdK"
                     /locus_tag="PK224_03100"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRB14754.1"
                     /translation="MAKKYVYYFGDGKAEGTGNMKELLGGKGAGLAEMTNLKVSVPPG
                     FTISTEACVEYYKRGKAYPPGMMDEALHALKRIERSMKAGFGDPDNPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVHGLALKTKNERFAQDSYRRFIGMFGSIVMGVNREHFEDI
                     LKHKKRDLGVTQDTHLDAKALKELVVSFKELVKEETKRDFPDDPLEQLRMAINAVFSS
                     WYGARAVTYRRLYNIPETWGTAVNVVAMVFGNMGETSGTGVAFTRDPATGQRQFFGEC
                     LTNAQGEDVVAGIRTPLPVKELEKFMPEAYKDLEATYKKLERHYRDMLDLEFTIQEGK
                     LYMLQTRVGKRTGVAAVRIAVDMVKEGLITKKEALQRIGPDQLAQYLYPIFDAQEESR
                     CTPLGKGLPAGPGAAAGKLALTADRAVEMKAAGNRVVLVRQETSPDDIHGMNAALGFL
                     TARGGMTSHAAVVARQMGKVCVAGCEAIEVLDSQSVRIGTQVFREGEYLSVNGSTGNV
                     YGGDIPVVESEVIQVLQGKMEASASEKYQLFESVLKWADGVRKLKVRANADVPDQARI
                     ARSFGAEGIGLCRTEHMFFAEDRIQIMQKMILARKREEREMYLDQLLPLQKQDFIGLY
                     REMKGFPVTIRLLDPPLHEFLPKREDLMVEIAQLELTSGAPTVLEEKKRLLARVEELH
                     EFNPMLGLRGCRLGITMPEITKMQARAIIEAACELAKEGTKIVPEIMIPLVGMVSEMK
                     AQKDLVREVATDTMKRYGVKLSYLVGTMIELPRAAVTADRIAEEAEFFSFGTNDLTQT
                     TFGFSRDDAAKFIDFYKTANILDSDPFAVLDREGVGSLMRTAIVGGRKTRPTIKLGIC
                     GEHGGDPSSVEFCHQLGLDYVSCSPYRVGIARLAAAQAALAEAETEKAKPKKVQPSTK
                     RVAKSKPAKKSATHAPVKKRPAAKRVVRRPKRTKR"
     gene            74290..75417
                     /gene="ribD"
                     /locus_tag="PK224_03105"
     CDS             74290..75417
                     /gene="ribD"
                     /locus_tag="PK224_03105"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRB14755.1"
                     /translation="MRLSSRDREYMTLALRLAAKGRGSTSPNPMVGALVVTRGRIVGQ
                     GSHRKAGGPHAEVIALSQAGSRAKGGTLYVTLEPCSHLKKRTPPCVPLVIASGVRRVV
                     VAMVDANPQVKGNGVAQLTRAGIRVDVGCCEAEARQLNEAYLHWVQTGRPFTILKAGM
                     TLDGQIATAKGESQWITDEAARQQAHRLRADVDAILVGIGTVLRDNPQLTARLTDGPA
                     RLAPRQPLRIVVDSRLRIPLKAKVLQDQQTAHTLIATTAAASGRKIALLRKRGIDVLV
                     LPKAGGHVNLPVLWARLGQLGVTTVLVEGGSDLNAAVLQAGLPQRLMWYVAPLLLGGQ
                     DAKGIFGGQSPHRLRNAVPLKNLRIEPVGRDMLIQADFSTR"
     gene            75425..76756
                     /locus_tag="PK224_03110"
     CDS             75425..76756
                     /locus_tag="PK224_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14756.1"
                     /translation="MRHSFRPFDRFHQRRLFLLPVLFSCLLWASLSIPATHAAEEGAS
                     TDLASQVWQYLTTQDAEQQRTVLASIVQRPDATVQAVQELLRKGPPHGLQPVGLLPDE
                     QIVVRERPYQLSLSVPQSYEPTRDYALIVCLHGAGFTGEAYLDRWKSRLGEGYILACP
                     TYPAGAWFTRRAEDLVLATVQAAQQRYRIDPNRIFLSGMSNGGIGAWLIGMHHAPLFA
                     GLAPMASGIDDVLFPFLENLRTTPTYIIHGSQDQVMPVELSRKLAGELKNLGYPYIYR
                     EHDRTHAMAGGHFFPREELPDLVKWFDDQRRTPVPKALTVVRDASHLLPFGWVRIDAT
                     DQIASFSEDLVDKRDVSIRQRIYARLTAQVTGPNRIEVKTDRVRQYTLFLNEDLVDLS
                     KPVVITTDGQVSFEGVVTPQVDTLLRQARLRQDPGQLYPAHLTLSLPQRPS"
     gene            76753..77559
                     /locus_tag="PK224_03115"
     CDS             76753..77559
                     /locus_tag="PK224_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14757.1"
                     /translation="MSRFRPVARRAGLIGVVLTAGLAVLASVGRTESDRCSLPAHHAG
                     VVFPLDQVEAAWACRLEPIVTHYTTANKVGPQRTPLPQPVFLYLLDHPVMAAMLVNRL
                     DLGLYKAEQRGQEEFWATDGEGTEGIVHSLFRDPTTRIYYVEGSHDGRFLPRVSGKAV
                     VLLRLHPVTDNRGVESIDSTMVAYLRLDNRFLSGMLSLLRPLIGNVVNRQILKAFDAA
                     RRLGGAMREHSEQVLFEATDPPALPDDEVAFLKAALAGLHNPVESLRPTP"
     gene            77570..78745
                     /locus_tag="PK224_03120"
     CDS             77570..78745
                     /locus_tag="PK224_03120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB14758.1"
                     /translation="MTTSRSFFYLCTLGVFCFISYNLVRMPVLSLFAESLGAGPERIG
                     LIVSVSTITGVLLKLPSGALSDIYGRKMLLRIGVVAFGLPPFIYPFISDLNALTALRF
                     VHGLATAIFAPSALATVADLYKERRGAALGTYTACTQSGSLLGPFLGGWLAYTAGFST
                     AFVTAGVFGCIAILIFFSLHLDEPPPRVRERGLAPVMAEMGKGFLAVARNRKVLITSS
                     TDAAKMVANGALMAFLPLYGLSVGLNAGQVGLLFSVQAVTSFLSKPVMGRVSDRVGRQ
                     PLILAGLLICAATFISMPHVGSFALLLVLSSGFGFGEAVVSSSSAALVADSSEFKRLG
                     AGMGMQGTVMDIGHASGPLLAGLLIAHVSYQGAFAVIAGLQILAAIAFWATMRTLSR"
     gene            78920..79576
                     /locus_tag="PK224_03125"
     CDS             78920..79576
                     /locus_tag="PK224_03125"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRB14759.1"
                     /translation="MFSGIVEEMGAVSVLNKGLAGTRLTIIASTIMSDLTIGASVSVN
                     GTCLTAVARTDHDFSVDVSPETLAVTTLGCLTSGSPVNLERAMKLNERIGGHMVSGHV
                     DGIGAIRSRHQDGNALILEIEAPKEILRLCVQKGSITVDGISLTINDVTERSFVVSII
                     PHTAKVTTLGLKQVGDKLNLESDLIGKYVERLLQERGILPPKPTPVIDTDYLKRRGLI
                     "
     gene            complement(79658..80176)
                     /locus_tag="PK224_03130"
     CDS             complement(79658..80176)
                     /locus_tag="PK224_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRB14760.1"
                     /translation="MQRQSEKQSVVWSTVMGFVLLGGVIPVAAAEDTKKPAEQAAPAA
                     KKTPEKKATAKSGSQKAKPAATAKDQAPKYTSAETAAKAQACFGEAPKIQSVTPDEAK
                     AGEKVTISGRGFGPADCLRSLSFGPGYPAAFTFVNDTQITATVPTGRRTGMAMMTVTT
                     ASGEDSKAFLVK"
     gene            complement(80232..82121)
                     /gene="acs"
                     /locus_tag="PK224_03135"
     CDS             complement(80232..82121)
                     /gene="acs"
                     /locus_tag="PK224_03135"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRB14761.1"
                     /translation="MDEKIDTLLKEGRVIQPTAKTKAAAHIQDYEQAYQASIADPEAF
                     WGGVAKELDWFSPWSKVLDWNYPWAKWFVGATCNIAYNCLDRHANTWRRNKVAIIWVG
                     ENGEERIFTYGELLRQVNRCANALKALGLQKGDRVTIYLPKIPEQVVAMLACARIGVI
                     HSVVYSGFSAPALASRIQDAEARVVITADVGYDRGKTINLKGVVDDAVRTSPSVEKVV
                     VVRRESPGAALEAPKEIDWVEWLQAQSATCDAEALDAETPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYITTKYVFDLKEEDVYFCVADPGWVTGHSYIVYGPLLNGATILMAEGKP
                     DYPNPGRWWDLIARYGVSIFYTTPTAVRLLMKYGEDWPKKYDLSTLRILGSVGEPINP
                     EAWEWFYRVTGSDKPIMDTWWQTETGSILVTPLPSVPLKPGSATRPFLGIEADVVDKQ
                     GNSLPNNAGGFAVIKKPWPSMMRTIYKDPDRYKVYWNTIPNCYTAGDVCRKDNDGYMW
                     FMGRADDVIKVAGNRIGTAEVESALVSHEAVAEAAVIGKPHRTAGEAIKAFVILKQGY
                     QDSKELVQSLKDHVLKELGKIAVPQEIDIVPSLPKTRSGKIMRRVLKAKELGQDVGDI
                     STIED"
     gene            82349..83734
                     /locus_tag="PK224_03140"
     CDS             82349..83734
                     /locus_tag="PK224_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRB14762.1"
                     /translation="MRSSTFPHLRRMFVCLVAMCAAGTVGILSIAHAQVGKPEGLYYK
                     SWAVVVGVENYLVAPKVPGALESAHAVAAALRGLGFDEVIELQDKEASSKHLLQILND
                     YLPRKVGRQDRVLFFFAGHAGVTQDSQSKELGYLVPWDAQLNNPAKAITFDQLKEFSR
                     RSASKHILMLFDANIRGWEVTAPQPLSLEGRLSPEDDTEKRAVQVLTAAEKEEAVGRA
                     PGQSAFVTALVAGLNGAADLNKNGWVMASELAAQVKQDVEAATKGAQHPQFVQLEGDG
                     DVILIEGRKGAFQAGKEPTSEADRMREARIQYEQAFALLQQQKSAEEALERLNRAIGY
                     DPSFGDAYVLKSYIRLEVLPNLEESLLAARAAVQHAPQNPDSHYSLALALTKKGQYQE
                     AKQAMQQALVVNPAYGDVYFSLGELYADHLNEPQKSVDAFRRYLELGGQSERAIRAVQ
                     GSAPPADKPAP"
     gene            complement(84002..84172)
                     /locus_tag="PK224_03145"
     CDS             complement(84002..84172)
                     /locus_tag="PK224_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14763.1"
                     /translation="MADVQIEDGIIKVVQLDIQDPKAAAVLAEYPQVRWPEITRRALK
                     IGLGYLKGGGKD"
     gene            84588..85154
                     /locus_tag="PK224_03150"
     CDS             84588..85154
                     /locus_tag="PK224_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14764.1"
                     /translation="MRRVEGAFSKFTGSALALVLLIGLTACGGPPNWVKKGSGAFNEK
                     SDKSFYGVGSVVGVRNEPLAWDTAENRSRAEIAKTFETYTAYLMRDYAASTTAGDFSR
                     NSEEQNIERAVKTFSAVTLNGVKPMDRYKDEKSGTYYVLTKLSLEDMKNNLEQAKELN
                     GQVRDFVRKNADRLFDRLEKEEDKRATK"
     gene            85193..85822
                     /locus_tag="PK224_03155"
     CDS             85193..85822
                     /locus_tag="PK224_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HRB14765.1"
                     /translation="MIEWRDRSVGRTFATAALVAVAAAASGCGHETKVTRVDTGIVTD
                     LSGRWNDTDSQMVAEAMVREALGNPWLGNFTKGKSRQPVVIVGTVLNKSHEHINVQTF
                     ISDLERELTNSQKVTFVAGKGEREEVREERREQAVHAREDTQKAPGKEIGADYMMRGS
                     ISTILDEQDGAKAVFYQIDLELVDMENNVKAWFGQKKIKKVVEKKRTIF"
     gene            85903..87333
                     /locus_tag="PK224_03160"
     CDS             85903..87333
                     /locus_tag="PK224_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14766.1"
                     /translation="MRVVFLGLLWTLSGCGFSANHYLLIDQSLLANDPRRADAIMAQA
                     ESEYGSRNRLLYDMDRGMTLHLAGDYVQSNSLLEQASLEVERLYTRTIRTETAAFLTN
                     DTMLPYEGDPYEHVMINVIKALNYAAMGQLMEAVVEARQIDHRLNVLSDSAKEKDGYR
                     EDAFARYLTGVLYEATGDLNNAFIAYRKAYEIYESTRGWARTPMPPMLRADLLRTTDA
                     LHMTAEFEEYRRQFPDTGWVSLQGQPDLAQVVVIGYNGRAPRKEEVHLDIPVSLGALQ
                     LVLLNRGVIHASNRQERRVADSVLYGLNGRVVRVALPRLVPQKTQVSHESITLVPRNG
                     EPITGQSQLMYNSTALAEKSLSDRMPGITTKALARAAVKFTAAEAATRGSQHAVNKGD
                     AAWVGLLVGLLTHGLAVASEVADTRSWRTLPDEIQISRLWVPAGEYESRIQPVLRSGG
                     APQAGVFRPLVLRAGHTVFLIERVVL"
     gene            87330..88451
                     /locus_tag="PK224_03165"
     CDS             87330..88451
                     /locus_tag="PK224_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HRB14767.1"
                     /translation="MKLVTGRGRAAVLSALLLLLAGCGWFGGTARPAWIDGGSPQFPS
                     AQYLVGVGQADSRPQATEQAYAAVSRIFKAEITAQAKDWESYLVVESRGQTSTERRLT
                     MDNVTRVTTDKVLENVQVLDTWFDQKTRQYYALAGMNRAQAEAAMVERLNELDRTIQT
                     EVTEAHQTQDKLSRVRNLKRAAKNLVLREAYNTDLRVLRSNGQGNASTYRVAELTAEL
                     EQFLAKNLVMAVDLSGDQAEPLERALVDGLAQEGFTVVGRGAGAVPAELLISGSVRLW
                     PIEVNDPHFRYVRWCAESVIEEVATHRVVGALSKGGKEGHVTEREAAAKAVRVMQQEF
                     ASDLARSVAAHVYGEMDLPASASTPSGCPKEAVQPPVSR"
     gene            88486..88734
                     /locus_tag="PK224_03170"
     CDS             88486..88734
                     /locus_tag="PK224_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14768.1"
                     /translation="MSKIGTRKSGEQGNGGRRLSSQAMKKRLRERLTADTQQVLKSDI
                     VSIFRKQGYYEELPLDELQDRLSKLQSPLADSLLKGRV"
     gene            88736..89179
                     /locus_tag="PK224_03175"
     CDS             88736..89179
                     /locus_tag="PK224_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRB14769.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRVVNKLMVKRGKVAILPMWSVTD
                     FYSALSVRAQQGEITRDDCYSVLYKFEMEASQGLFQFISPTMETYLAAKELILDYPAL
                     RSPQLLHLALALELKPLRLTVVSADKQLLAACRPAGLHIINPEDD"
     gene            complement(89607..90125)
                     /locus_tag="PK224_03180"
     CDS             complement(89607..90125)
                     /locus_tag="PK224_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14770.1"
                     /translation="MRRTLVACQILTVALIVCIAGNGQAQVMGEEAELDRLRAKAEDA
                     MGNDDAETAAMSMGRAALMAAQLSKRQTEPAPRQTFNATEHLYRSQEHGYRAIALFRR
                     AGGELPASAGVCGSLQLAQLELRHAQEALSGPNDTEGKNTASPRRKAAQQSMEDWSIV
                     LDSIQGEFRCPS"
     gene            complement(90164..91240)
                     /locus_tag="PK224_03185"
     CDS             complement(90164..91240)
                     /locus_tag="PK224_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB14771.1"
                     /translation="MRCILSLITLGALVLLGDQSWARRDALTAEQKSQLERIDRVLVD
                     VIVLSDKGSLDAKPLVDVVVTRMKEFDYTTVTDPTQPHDVELKIKCEQRKTWEGTTTM
                     GSDADLPDAPSRIWKGPACQLGYLLNGKKMAWRKEVRTDFDDAQAAATLAKAGDPTAY
                     AMAKLQARLEEYDFPALITAEWGQEARLFRLYDDPKTPVARKVKLVALFGDLFSVKAI
                     PRLLDGLNGADRSIAKASALALGNIGQKDTIPVLIKTMKSGPPDLRAPAAKALGIVGA
                     LHGDFTIVDPLLETLNTEDVAVKTEVAWALGKLPDMRAYEPLFTLQKSLYRVHENDAD
                     PNLVKLKEAVNWSIKQIDTWEHVQ"
     gene            91355..92185
                     /gene="proC"
                     /locus_tag="PK224_03190"
     CDS             91355..92185
                     /gene="proC"
                     /locus_tag="PK224_03190"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRB14772.1"
                     /translation="MLTGTRIAFIGGGNMAEALLAGLLRKGVAVAEHLTVSDPDVHRR
                     ELLAERFGVTVHADNRTAAQGAELVVFCVEPQVLDSVLDEVASSLQSRPLVMSVAAGY
                     PISRLQAHLKTATRLVRAMPNTPSTIGEGVTAMSMMPGLSSEDQDRARCLFESVGQVV
                     VVEERLMDAVTGLSGSGPAYVFAMIEALADGGVLMGLPRITAQVLAAQTVAGAARMVL
                     DQGTHPAVLKDRVASPGGTTIAGLSRLEQGRLRATLMSAVTAAAQRSQELGKEENHHL
                     "
     gene            92201..92395
                     /locus_tag="PK224_03195"
     CDS             92201..92395
                     /locus_tag="PK224_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14773.1"
                     /translation="MQYWVKVVFADNQELLVKDAIRHTISEDMEVLEVDSAKEVIIVP
                     MKQIKYIACDATVFAQKAKA"
     gene            complement(92556..92888)
                     /locus_tag="PK224_03200"
     CDS             complement(92556..92888)
                     /locus_tag="PK224_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14774.1"
                     /translation="MNGLPLRFKNQGLIERYQMEGMDPSDRSFNRTVYVNRTSVGYAG
                     KVIYEAFVVEGTDQPTTGAAVRAVADKLLGFGFTRLRSRLNFKGARYLAEKETWVDYP
                     DLQPKVPS"
     gene            93792..95057
                     /locus_tag="PK224_03205"
     CDS             93792..95057
                     /locus_tag="PK224_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB14775.1"
                     /translation="MNCQKIMRKFTANGGSVRGESQGRYRTLFELAPIAVYSCDASGL
                     IREYNHRAVELWGRTPAHGQTNERFCGCLKMYRPDGSFMAHEQCPMSDVLSGKVPEIH
                     DAEVHIERPDGSRIIVIANIAPLTDDQGAVTGAISCFYDITGRKQDEKALHVSEERYR
                     HLYGQLESRVAERTEELAESQKRLRVLVAALDVTEERERQRLAGNLHDDLAQFLALSR
                     IKLALAKQQPMQAPLANLIADVDEATNKAMVYTRTLISELSPPALSESGLPMALQWLT
                     EQMRQHDLSASLELKTQMPTIPKDHALLLFRSVRELLWNCVKHAGVSKAAITLEQLEQ
                     SLYIHISDQGVGFDPRSASETEYSPTHGFGLLSIRERMLSLGGRLVLESSPRNGTTAT
                     LVVPLRHAAVESSSGRARWSGPGVDGGEY"
     gene            complement(95276..95773)
                     /locus_tag="PK224_03210"
     CDS             complement(95276..95773)
                     /locus_tag="PK224_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB14776.1"
                     /translation="MAKQSATVPAVVEMLKDDHKKVKGLFDEFESAEGGEQAAIALKA
                     IHELEIHAELEEALIYPAIREAIDDADMMNEAVEEHHLVHVLIAELKKLKPSDERFHA
                     KFCVLSELVKHHIEEEEGEMLPKAEHHDIDWDALEVKVTKRKLALLAKPDRTAKKTTA
                     AKSSR"
     gene            complement(95937..96743)
                     /locus_tag="PK224_03215"
     CDS             complement(95937..96743)
                     /locus_tag="PK224_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14777.1"
                     /translation="MTTFQSKSVAIFALACMLPVAGARAAPEEKHIDHEHLQGNRTFL
                     GTVDEVRSDQARVNTGEGQPRFVPMNVRKDKGLPDLRIGDLVEITVNDQNLLVDVHKA
                     GEISHHRIVRGQLAVTLGTGHETAVIRTAEGKEESHGIRPVARSKVSSLPVGAEVTFL
                     LDELDTIVDVTLGTPHRAGQSRTQGHKQSPIKGNLRQVRGVILKALQDKTIAIRTEDG
                     KEQSFEVRPLILERLSKVSRGQSAVLLIDEAQQVADVSFNHSDKGSPSTH"
     gene            complement(96838..97344)
                     /locus_tag="PK224_03220"
     CDS             complement(96838..97344)
                     /locus_tag="PK224_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB14778.1"
                     /translation="MLLTQDSELREQLTEALQQSGHKVAIPAHRENILTMLENSRPSL
                     IILDLHVSHPSGPDDLKMIRDRGYVERTIVLSSPPMISALTESHANGVDRVLKVPVKI
                     NGRYDLGELRAAVKSCLQNRAASARPTAHGAIAQRAYELYEAGDRHEGSNLQHWLQAE
                     RDITMRQQ"
     gene            97656..97952
                     /locus_tag="PK224_03225"
     CDS             97656..97952
                     /locus_tag="PK224_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14779.1"
                     /translation="MNKQVFAKRSFLGASVLTLTFSLSPFLTHAEESQVKGAMITVPT
                     NTQSDVAQGAVEDSLKACLARIPDKASAGQRMLAERTCQGEEAIRKTTHGGPQF"
     gene            97997..98242
                     /locus_tag="PK224_03230"
     CDS             97997..98242
                     /locus_tag="PK224_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14780.1"
                     /translation="MNQEQFEQFWLQLKMPLKAKWERITDSDLGEIQGNLATFKDVVL
                     KRYGDTNTSEVSIWVDRRYSHWTGNYLGYRDPEPASS"
     gene            98326..98670
                     /locus_tag="PK224_03235"
     CDS             98326..98670
                     /locus_tag="PK224_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14781.1"
                     /translation="MVKIVINASYDRFCVSHKAFLRLRELGQPDALNETDHGAYWPQA
                     ASPQEPSLNRCGILIPRDDRRLVQVVEELRGEANGHCAELKVVTIPDDVEWVVVKKDG
                     REQVSEQHRTWS"
     gene            complement(99498..99668)
                     /locus_tag="PK224_03240"
     CDS             complement(99498..99668)
                     /locus_tag="PK224_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14782.1"
                     /translation="MSTTTRPADGEAMDQRCAICPFCQETGFDAIGLKKHLVLWCLTF
                     KQLPPLQRPFYG"
     gene            100009..100662
                     /locus_tag="PK224_03245"
     CDS             100009..100662
                     /locus_tag="PK224_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRB14783.1"
                     /translation="MAAKRPTKFNPKTFLGKVGTGKTNLTLEKHHTIFSQGDEADAVF
                     FVRAGKVKLSIVSQQGKEAVLALLEPGAFFGESCLAGQIVRMATAVAEEASVLVRIEK
                     TAMIQVLHKDPTFSELFMSYLLLHNMRIEEDLVDHLFNSSEKRLARVLLLLAHFGKDG
                     KPESVVPKVSQETLAEMIGTTRSRVSFFMNKFRKLGFIKYNGKLHVHSSLLNVVLHD"
BASE COUNT        20327 a        28373 c        31140 g        21532 t
ORIGIN      
        1 atgggtggcc gacttgcgtc ggaatcagcg gccggcttgg gtcggaatac gcagtctgcc
       61 tgattctagt aaggattcct ctcgaagaac ggtatttatg acagggtgat actcgtcggt
      121 gacgccgtct agaaagtctc gcaccatgtt taacgcagcc atggatgaaa ggttcgtgcg
      181 aatatcagtt ccgaggaaat aagcaccggc attctcgtag gtcacattgt ccgctgcacc
      241 gacgatctca aatcgtcgct ctcccatttc caacgactca tagtgctcca atacgtatgc
      301 gaggtcacgg aggtctctgg gaaacttcct gtcaagatag gcaaagattt tcaggaccga
      361 gaaaagccag agggggataa tcggaatagt aagacttttt gttaattcga ccggtacggc
      421 atgctcaaat acgtcccaaa atcctgccag gttcatgcga ttgccggact tgcgccatac
      481 caacacttcg tccgggccat tggcgatgcc atatgggatg aggtcgatct cagccgttcg
      541 ataatgccgg cggtgttctt gttcgctgcg gcttcgtacg aaccctacgg tgacaagatc
      601 ggctatcaca gtctcccatt cagcccagga agacactcgg atcacatggt cggcatccct
      661 ggtttctcga actcctatct gatatggcaa caggatagcc ggcacgagcg ccccgatgag
      721 cgcgaagggg atcttctttt attcgaaata cttgtggagc agggtcagcg ctgttgccag
      781 atcgccttca atctttggca atggagtctt ccaaaaattc tttgtacatg agttgggccg
      841 tttcacggtc acgatccgtg ccgtggtgta acaactccgc gtaaatcaga atgggatgtg
      901 cggcaggaat gcggtgctga ctttcaggtt tctgaatcac gcgcggggaa aagcttttca
      961 agatagtaat gggcccccca gaaaccggta accacttgag atttttcaag gcctccttct
     1021 cttgccacga ttcgacgaaa agtgtgagcg agttcccctg ataatggtga atgatgacgt
     1081 cagctccgaa tcctcccgtg agagaccatg ataatgcttg ctgcattgca tagtggccga
     1141 acgtcttcgc gaccgtagga aggtcattcc tcagatctcg atactccccg gcaaagcact
     1201 ttggacgaag acggctggca taaccttgca cccaccgatc gaacagttct tttcgccgaa
     1261 tcaaacgaac gcgatctttg gcagtgggtt cgagataccc ttgttcgagg agatcccgtt
     1321 tcacccatcc aaccgtacca agggcgaccc cattctcgcc tgcgagatct cgataggggc
     1381 ggttgatgga ttcaggttct acaagtagac caaaaagaag cgtgaggcca ctcggttgga
     1441 agagcctcgt tggtttgtcc tttggagaga gtggcggcct tttcccctcg atgtagaggt
     1501 agagccccgg cgagcgcttg agaaagagat ttccggccgt atcgatgaag ttgacaccgg
     1561 cttgtttcag ctggttggcg agaactgggt tgatatagtc agcgagcaac aagagttccg
     1621 ttgccttgcg ccatcgtttg cgatcggcat gtgcccggat gagtaagtgg tgaatcgctt
     1681 gcggacgaag atgcgtcttt acctcaaaca ggtaccgata ggtctcaccc gtagttttga
     1741 ttgtcaactc accatcccac ccctgctcgc cctctcgttt cacattcact atacgaagag
     1801 ggtggacagc cttgagttgc tcgaggctgg aagtgacaat ctttgattct tgcgcgagga
     1861 gcttcgtcat gtctggaaac atatcacgtt catgaatcgt gaacaatgcg attcagtgaa
     1921 cagatcgatc caccgaggcg gcggcgcaaa gttgctattg aatgtgcaaa atcatgagtt
     1981 acggtccttc gcctttcggg ccggtcgctt caccggcacg tgctcggccg acagcagggt
     2041 ctgcgcctgc gcgatcgcat ccagcagagc gtttcgttct ttgtccagct cttgcacgta
     2101 gggcacgccg ttcacgaatt cttccgctgc cgatatcaag gtatgcatcg ccgcagcaat
     2161 ctcgtcctgt gcagcagggc gggatggtgt ggttgcacgg gagagggcgc gccttcttct
     2221 gggcccgaca tacaactgcg gcgcggtcgg gttatctttt tccaattccg gtcgtctcga
     2281 tctcgccatt attcctccag gtcatcaggc accgatgtgt gacgcaaata ctctactcga
     2341 tccttggtgg cgattgcatc ggggcgaaag ccgaagggaa ggcgatccgg aggactcgtg
     2401 aaccgatcca catccgctga ctgcacactc accagagata agacccgaat aggcaagggg
     2461 ttcatccagt cgggggatgc aacgacacaa gcggacgtcc ggctcccgcg gtcttgggat
     2521 cgtacggcgc gatgactccc gccagaccgt cccacggaat cacgtgggcc atctggtccg
     2581 agaatcgctc gaggcgagag gctggcgata ctttttagaa tggagggtcc gcaaagagct
     2641 gttgctgcat ggtgggcctc ctcagcggcg ctggcccagc cagaacggaa ctcccgaggc
     2701 taacaaatca gagatttcct cggaagatca cctctctgca tttagtgtat caattcagat
     2761 tcagcgcagc tatctgaaga gatacagtgt gtagcacagt atttcgccag aagccgtcaa
     2821 gcagctcaat atattgttct acacaaaagt gattgcacac tatgttgatt ttgcgtgccc
     2881 aggatacggg gcagctcact tgtggcaagg ttgttgcccc actccgacta cagtgtggca
     2941 cgtaaggcga agacggtttt ggtcgtgatg ctggaaggca gcgtgacagg ccatcaacac
     3001 ctcgttcaag atcggtcatc tcttccagaa gaaatcggga aactatcgaa tcacatctcg
     3061 accttgccgt cgtctctatc gctgccaagg tgaactgctt gaaaaggcac gagttgttcg
     3121 acggagagat cctgacgaga ttctggctat acagtcagag agtaagagat tcgagagtct
     3181 atgaaggaaa gacatcagac cgaatgtttc gtatcgtatg gccgtagcga tgcgattcta
     3241 cattgaagca cgcacgaggc agtaggattt cagcccgccc atatttgcag atatgtatcc
     3301 agcccgcacg caggaggggc cgatatgcgt gggtggcacc gtttttttag tggcggcact
     3361 tcgaaggtcg cgcacaactt ccatgatgcg tttcagcgag caggtgttga gctatgcccg
     3421 ctccagcatc ctccatcggg acccggccta gtgatattcc aggaatgtga ccctggcctg
     3481 caggactttc tacaagatgc acggcaagct gggcttcaca gaattatcgc catcagactt
     3541 ccaccgggag gcttaaccca gtcggtagtt tggagactct taagggctgg cgcctgcgat
     3601 gtcctagact gggatgatcc ggatcggacc gcgcacgcag tcgcagcacg actggtgcga
     3661 tggaagacta tcgatgagct ggtcacatct tcagaggttc aagacacgtt ggtgtgccga
     3721 agcctagcag ccttgatagt actacggcag ttggtagagg ctgcatattt caccgattcg
     3781 atgatccttc tgatgggaga gagcgggact gggaaagaac ttgccgcgcg cctcattcac
     3841 tctctggacc agcgcgcgaa caaaggtgac ctgattatcc tcgattgtac aacggtcgtc
     3901 cctgagcttt caggcagtga gttcttcggc catgagcgag gggctttcac tggcgcgatc
     3961 acggcacgag acggtgcctt ttcgcgagcc aacggcggca ctttgttttt ggacgaggta
     4021 ggagagcttc cgctgggctt gcaagggcag ttgcttcgcg cgattcaaga acattcctat
     4081 aagcgagtgg ggggcaacac ctggcatcgc accgagttcc gcctgatctg cgcaaccaat
     4141 cgggacttga agaaagacat tgcgcagggt cgtttccgta gcgatctgta ttaccggatc
     4201 gcgggcgatc tgttcaccat acctccgtta cgcgagcggg ccgacgacat tcttccgctg
     4261 gctcgtcatt tcacacggtc attggtgcct gacttagatc cccccgagtt cgacgagacg
     4321 atgagggagt atctcacgct ccgggaatat cctggcaaca tacgggaatt gaaacaactg
     4381 atcatgagga tcatgagcca ctatgtggga tttggaccta ttacggcggg ctgcctacca
     4441 gagggggaac gaccggaggc cgttgaagag cagcgggaat ggtctaatca agagttcgaa
     4501 cgctgcattc gtagcgccat cgcacgagga ttcggactga aggagatcgg acggatggca
     4561 gagaatattg cagtgcgtat cgcggtagga gacgaacagg gcagtctaca acgtgccgct
     4621 gcacgcctaa gagtgactga tcgcgcacta caactgcgcc aggctacggc ccgttttccc
     4681 aacgggcaat ccgaatgctg actccggcgc cgatgcgtcg cttcatcaat aatgtacgtc
     4741 tgtagtgctc ccctgaaatc aagccaggtg gcttgatttc aggggagcac tacactggat
     4801 cttctcgccg aaggaaatat ctgtgtagtc cttgatcttg cagagaacga gctggcattc
     4861 atcaccgtca ggtcagcagc aagcaaattg ctcaccggcc cagggatcgt ggacctcgct
     4921 ggagatgcca atcccgtcgg gagcacggct ggcgacacca gtagatcgca caggataccg
     4981 gcggatggat gagatcagtc tcagaaagtt gcgactcaaa atgaggtcct cttcattgtc
     5041 aggcaaacgc agcgcgtaga ttttggcaag ttcaacgccg ggatgaagcc agggcccgtc
     5101 ggacccgaac agtacctttt tagcgcccgc tcggctcacg gcctgatcga gcagatcgaa
     5161 ccgccgtacc ccggccgtat cggtatagat attgggatgc cgggccagat gatcgatcaa
     5221 ggctagttgc gcgcgccaat catcggcgaa gctgcccaga tgcggaatga tgaaatttac
     5281 atcaggatag tcctgcgcca gcaactcaca cacagccact tctcccatca catcatagag
     5341 aacgggcaat cgaaatgacc gtgccacctc gcaaatttca cgcgtgatcc tggcatcatg
     5401 acggtggacc ttgattccga caaagccgaa ccgatgtgcc gcctcatgaa ccatcgaatg
     5461 gatacgacca cgatcccgct ccgcatgcac gaaggcgaat gcgtagaagc gctcaggacg
     5521 gcttgcgaca atgcgagcca cttcacgatt cgcctggcgg tagtcggaat gaaaggcagc
     5581 cagcaacgca gtgcggttga tcccagcacg agcagcccgt cgcatatact tatccaaggg
     5641 cgcacgagta tcccatggac cggtcagtcc gtctccggct cccgcgtggc aatggcaatc
     5701 gatgatcatg gcctttctca ttcgccccta catggattgc cggtttcata accagctcga
     5761 tttcccagtt cgtcacctac aaacggctgg tgctccgtcg taggctgtgt ggagaatgat
     5821 tcccttcgac ctcattgttc gcattttcaa tgtgctagtg ttcactccgc gtcgacagca
     5881 atgaatcact cctgaatgaa attggctttc attgcttggc aacctggatc agtgtaaatc
     5941 ggggattgaa aggcagtaca tcatttgcat aaggtgcagc accggcaata ttccgtttca
     6001 gcagcgctac tcttccctga acagagcggg ccacaacttt gccattgtgt tgaatcggtc
     6061 tcacatagtt ttttaatccc cagtatgcat tcagaattgc ccttcccaaa tgcttgaaat
     6121 accttccgcg gaattctccg gtatttcctc tttcatccat atagccctca ttcatgattg
     6181 ctatgtcatc ctttgggtta tcgcgatttt ttaccgacgg cctcctcgag tacccgaatt
     6241 ccgtcgactc tgagccggtc gataaacgaa cgaaacgcat ggcgtacacc tcggacggtc
     6301 tgccccttag ccgaaaccgt gtccggtaca tctcgtaaac gagcaaagag ttcatcggcg
     6361 atgccatgtt ttactgtgat atccacttcc cgatatgcga catttcccaa gtttccccat
     6421 cgccattcaa gcggcgcaat cccggagcca cttcccggca gtcgaacatt ggcgttcgct
     6481 gtgagatcct ctcttgggac gaaggctttt gctgcgaaga atggcaatcc catcgccttc
     6541 ttgacggcat cggctcgctg tgatgaactg agcttctgcc ccacagcctc tttgccggct
     6601 gcatagagtc gttcgagctc tcgataagat gccttgacgg ccttcaccac catcattttg
     6661 gtggttgcac ccttaatcgt cctaagatgc gcgtctccgg cagccgtcca gcgatatccg
     6721 cctgtgacct tgttgccgtt atgatccaca tcagaaatca cgcgaagacc gcggttgtcg
     6781 aagtcgtgca gagctaggct tacgatatcc cccagggaaa acgtccgtat cgcttccccc
     6841 ccaagttgct tgacccttag tccgattgtg tttctggtta cccacgataa ccaccaggcc
     6901 agtggtggaa gcagatttct cttgtcgaga ttgctgtaca tgaatctcgc catgtagttg
     6961 atgaatcgtt gtgaggaatc tgggtagtgt ttcccgtacc aatgtcggat tgcaacccgt
     7021 ggggttcgaa cgtgtcctgc cgagaagatg tcggtcagga agtgacaggc aaaaccctcc
     7081 ttcgaaattg cccgttgcca ggtcttggcg tcggactgtt cgccggcaaa aaacgcatcg
     7141 gtaattgccg cggagtggga tgcgatgtaa gtactccacg ccgttccacc tgcggaaaaa
     7201 tgtgaaatgt tctggcttgc gagaacgaaa tatcgcacac gaactgcgtc tttaacttta
     7261 tcggaaacct ttggctcttg ctgtttgggc atatcgagcg cgcgccatcg ggcaaatgcg
     7321 atatttgccc ggccttcctg ggttcgagaa agccccgcca tctggaaata agtcgcaaaa
     7381 tagtcgccgg caagcgctat catctcaccg aatgtcaggc gtttgccgct ttcgtcataa
     7441 ataatcgacg tcggtctttg gcccaacgcc aaatcgccga tatctcgatg ctcacttccg
     7501 acaaatcctt caaggctatc ttctgattcg tcggcctgat aaatctcgta acttgctcgt
     7561 tcccctgaac cggtttgaaa ctcaccttca caccggttgg gctggttgcg aaattcctgg
     7621 aaactgaacg gctcaacttc gaatgatagt ttctcccgca tattgtatcc taattctatg
     7681 taatgaccgt aacgaggtcg tcgtgtgagg caaaatagtt actacaaatc tttcagaacg
     7741 ttggccccct gaattctctc cttccagtgg ttaatcggca cccagttatg ccctcgtgac
     7801 gattgctgca cgaccacatt cggcaggttc ttggcttgga gtttcttgga caaccgctcg
     7861 gtgctgcgca ggtaatacat atacagccgt cttcgtgggt cttgagaagc ccacgttgcc
     7921 cagagatctg gatcgccgcc gttgtagaga caatcgaatc cccagcactc gcggatatga
     7981 ggggcatacc gttgcgtact gagcgctagc cggcgcatgg gactgccgcc gccggaatgg
     8041 caggccagaa caatgttccc gatccttggc gattgggcgc tccctttgta cggtccgtgg
     8101 ttcatgagtg cttgcaagac ctgatcgaga tagcgatcta agccattggg tctcagcagc
     8161 cacccggctt gagatttggg gcccaacgta ggggccacaa gaatgacatt tttcccggcg
     8221 tcgttcagtc cctctcgaag cggaaaatag ggtacgcgtg ctttgttcca ataggagtca
     8281 atgctcatcc gtgagttgta gccatacaac ttgtacccgt ggaggtagag aatcaggtct
     8341 gcctggatct gtggtcgata gcctgacggt agaaagatgc cagtcattgg ccgcgccggg
     8401 ctttctgtgc cgagcaaaat attcatatac aaggattgcg cgggaggttg cgtttcctgc
     8461 ttgatcaagc gcggcgcgga cgtttgtctc ggtgctccct tgaattccca ttccgtctct
     8521 gccgacacag gaggcaatgc ccttgcttcc cgtgcgaatt gtccgcactg acatctcatg
     8581 aagatcctcg ctacggttgt cgaccaggtg cgcggcgcac gtggccctta catgctgtgc
     8641 ccacaggtcg gacaacttcc tgtctgccag gcttcaaatg cctctccagc tgcttcatcc
     8701 tcatagacct cggccccttg ccattcttct tcgtatgtct cagccgcaag ctcagcttcg
     8761 cgtgttgccg gtgggttata ttcccagtgc cacggctcgc gttcgtatgg gtagaagcca
     8821 aaccgctcgg cgttcttcat cagccaacga taggccggca gatttctcat ccgtgcggca
     8881 ttcttgctcg aaatggccag cccgatatag aaatcaatcg cccgcccgct ttgatgtgcg
     8941 ctggtcccag gcggggcgac ccacctgcgc gctgcctgtg gtgatccgta tctcaccagg
     9001 gctttgcgcc agatttgttc ttgccgcttc ggatcccggt accctgacat aggcaacagg
     9061 agcggcgccg caatcccgtc ggcgcgcgcc gctgcgacca gcgcgcgcca ggcctcggcg
     9121 gccagacgat gtaatggaac ccgacgcttc tggtatcccg ccacagtcac gacatcggtg
     9181 ggattgggag cacgtttgtc cgtgatgcgg cctccgccgc tgcgttccac aggtgtcgga
     9241 gtgaactcac cacccttcgg ctgaatctca aatggctccg cttcaaatgc gagtgcatac
     9301 cgcatgattc acctcatcgt tgtctatcga ccgatggtgt cattgccgtt gacagatccg
     9361 gattgcgacc cgcctattac gttgtcgccc ctccgccgtc gcatcggagg cgacgggttg
     9421 actaccgtga aacgaccggg tgacggtttc aatgtccaag ccgcgatccg cgagaaactt
     9481 cgccaccgcc agcgcgcgtt gaagcgccag ggatcgcctc gagccttctc ccggcgatcc
     9541 gtccgtatga ccttcaatgg tcaacaccaa gtccgatgcc ggtgatcgct tcagactgtc
     9601 ggctaaccgt gccagcgtgg cgtggtggcc tggttgtaac tgtgcgctgc cggctccaaa
     9661 gttgtgcagc acggtcggcg gccggcaggt ctccgttggt gcagatgcgg ccgtatcgcc
     9721 accggttcct ccggccgatg atgtaggttc cggcgttgga gaggcaggtt cggcagatgg
     9781 ctgatcttca gccggtggcc atggcggtgt tcgcacgcca cggaaaggtg ggggaaacac
     9841 catcaccggc ggccttcgtc cgggaagcgt tggcagtttc gaaggcctcg gccgcttcgg
     9901 ggtttttgcc cttggcggac gaatcttcag gggacgctgt ctcacagaag gacggcgcac
     9961 cctggatcgt tcaagctcac cctcttcccc aaaggcacca aacggctcgg cttcaaacac
    10021 tagtgacggt ctcatatcgc gctccttctc ccgaggtccc gccacccttc ggtgacctcg
    10081 cccaacggta cgtcagtcaa aatcgggcgg caacagtcgc tctcaacgcc gcccgcctga
    10141 accggccatt gcagggttaa ctcacgcgcg agcgcggtat cgtcaacgtg ttctgctctt
    10201 gtggagtccg cgcctggctc agcaaccgat tccggagatg gatggccggg agggtggtat
    10261 ccaccttccc cgtcacgtca atgctgagat cgacgcccgt cactacacgg taggcatgga
    10321 cgtagccctg gacttcggct cgccagaacc gcgcccagtt ggcggcttgt ttcggatcat
    10381 tcgtgtccga ccaggctcca tagcgaatcg ataacagcac ctgctcaccg aacacgccga
    10441 gattccggaa gtgcaagaca ttcacgtccg tccagccctg gagtttcttc atggcatcga
    10501 cccggtccat ccatggttcg ggataggcca ccatggtgcg ggctggaaga aattgccgga
    10561 actccggacg cgccagcagc cactgttgca tcagcatctc gactctcgct gttcccggca
    10621 gatcaccaaa ctgattgtgc gccccttccg acagtaacag gtgcacatca cgcaaggcat
    10681 tcaacagtgg gaagccatct gccaccacgg tggtgtcgtc atcttgcttg tagaattttg
    10741 cacatagatt cagcagattg tggaaggcct ccaagaactt ggagcgactg tcggccggcc
    10801 ggaaatcggc gaccgctttc ccataaatgc ggagaccgta gtggtggtca tactcgtacg
    10861 ctctccggac gactgacagg cggtgaattt catcttgcac atagctccag agcagattgt
    10921 ttaggggccg caacggatcg gtttccagat tggctagcgg atcagaaccg ctcgcgctgc
    10981 gtcgattctg gaaccgttgt gtgattgccg ccatcgtttg caccagcatg ccttcttcat
    11041 gccaatacga ccacatcaac tcaagaagac atggctgttg aagtttctgt ttgaggattc
    11101 cataacatct cttatctgta tcgtctgtat tgcttgctcc aactgacggg tcgatgaaga
    11161 tatcgttctt cagttggata tccgggaact tgcgcatgat tagttccagg tagggcagtg
    11221 tggtggtacc attgccattc actgtcctca gataatccac ccaaagggtg tggagatcgg
    11281 aaggcgatag actgacctcc acccgtttga gtacctctgc gctgccctca gagaaatccc
    11341 gctcggagtc aagaagcacc gcacaattgg tcagtacaaa tgcttcggtg gccactttga
    11401 ggaggcgata cgcgtccgaa tcagtaaagg gcaggaaacg cttctgcgtt aatccttgga
    11461 tgtggggatc agagctacct tgatatttgc tttggacatc ttgatttagg cgtatattcg
    11521 gattggcagg agggatgttg cacagcaccc aatccatgtg agctttgtaa ttgtcgaagg
    11581 acaatgcatc tgcgctgcct ttgatgatca cccaaagggg aagatctgga gaagctgtgg
    11641 gagtcgaacg ccgcatagtc acggcgattg tgtctcgatg ggccagctct cggggctcaa
    11701 tgtccagttc agaaggcgtt tcattggatt gaactatttc ctgatcctgg ccatttctgg
    11761 atgggtcttt tttgtatact ttgatgtttc tgtagcgccc tgctctcaag ttagatgtgt
    11821 cccacgaaat gaaggtttcg ctcccggccg tcttggctga tgcctcaaaa cttatgtcat
    11881 tgccgaatgg gtcagttacc ttaatcagga atggttcatt catattgact ggttctgaaa
    11941 gaatgaggtt gaccacagtt ccttctttga cctttgtctg atgcgctgca gtagaccaac
    12001 ctgccttagg atttcgtacc atggtgcaat cctccctccg tctggttact cccgttttct
    12061 tgtctattcg gatgttgctc ctaacttctt agccatcact cgctacgacc gctgctcgca
    12121 aggtatgagt gcgtgcagcc ctctcgtcaa tgcccaatgg ttcaagaact cgccgaccat
    12181 gcggctctct cggtcggcct ccagtagtcc atcgactttc gcctggccca gtacggctaa
    12241 cacgagcgcc ggcctggccc tccgcatgtg cgccgcctct gtccgccaca cctgccacag
    12301 ccttcgcagt tgcgccggtt gccgttcgtt gacaggcaag gcttctttca atgaattgcc
    12361 tcgcagtaac ttcggtcgat gattcaccag aatatcgacg aagctcggga ttgtggattc
    12421 gagaatcgcc aggagccttc gtccggctgc ttcgaggccg gtcgtcggat agaacgcttc
    12481 ccacaccttc gcgacactat cccactgagg gtccggatac agggcccgcc ccatggcaca
    12541 gctcagcttg acgcggatcc agggaacggg atgcggatcg ttcagcgcaa cccggaatac
    12601 aaaggaacgt ggcaggctga ccacacccat caaccctagc gacgacgaaa tcccgaccat
    12661 cgcgaccgac cagagatccg cgacgatttc cgagatccag cgttcccaga gcgtccacgc
    12721 gaggcgcatg gttccccctg tccgctgaag gcctttgaga atgatcttca gagatgcgac
    12781 gagatccagc atgttcgccg cctggtgacc gacttcatgc acgagtgatg acgcaatgcc
    12841 gctgccgacc atccgctccc tggggactcg aataatggct accggattct ccctcccgcc
    12901 gggaagcctg gtgcgcgctc gacgaatggc ggcccccggt cctcggtcca agtaacagat
    12961 gacaggcgga gacttgtaga atgcgccagg tatcgccaac gcatcggctg cgaaggcgtc
    13021 cagccctgcc agcaagaccc cgaggccatg ctcgcttcgc tgcgtcaaca catccgcaaa
    13081 aatatcgaag tcggacaata acgcattgaa ccgcatccga aggaacacga aacgccgatt
    13141 catctcggcc ggcgggacca ggcgtcccag tggaccgccg agccagcgga tataggcttg
    13201 aatcatgccc cgaagttgcc ggcgtccctc cgccacgtag tgctcgatcg cgccatgcgc
    13261 ctgaggcgcg ccggctgcgg ataccatggg cacgctcaac gcaaacggcc tgacgcggtc
    13321 cagccggccg agcagcccct gtgcttcatg gagtaacgtc gccgctgcca tcgcttgaag
    13381 gaccatgttc agactccaaa cagaatgatg acgcccccgc gacggatcca acgcccgcct
    13441 ttaccacggc gaccgggcat catcctcgtc ccggacggcg tcgcgccctt cagcatgccc
    13501 ggcgcataac cttgcgcggc cgtcgctacg gcgttctggg ccacggcctg cggaggtgcg
    13561 gctggagaag ccgtcgcggc ttgactcgtc gcggcgccgg ctaaccgtac taactgccgt
    13621 gcggcttcat actcctggtc ttcttggctc agcccttcga gctctaggcc gagcaggcgg
    13681 cccgccattg gcgccaggcg attgccaaga gccgcgcccg ccgggccacc gacgaatccg
    13741 cccagcgcgc cggcaaaggg caacgctttc ttgatggctc ctttgagata gccacccaag
    13801 gcctggccga ccggactctt gatgaatcct ccaactttcc tcgacgctcg gcgaatgagc
    13861 tttccaagga actgatcgag ttctccttcg ctggtcactt ccagcaactc ggcggcaaat
    13921 tccatctcct cctgttcttg aaaaggactc gcggctgccg tcgccatctc atacgcctcc
    13981 ccatattcag cctctccttc ataggcgtct ccggtctctt gatactcgaa tgcttcacct
    14041 tcgaagtatt cactgtctcc cgtttccatt tgcgtgcgat cgagatcatg catggtgtac
    14101 tccctccttg aatggacgac cggtggtggg cgtctcaaca ttgaatgcgt attcgctatc
    14161 gcaaacccag tgccagatat ttcttgggga tttgaaggag tcccgcgaag cccacttcgt
    14221 attctcacga catgaagcaa cgtcaggcga agagggattc gcgcgatcgg tccatcgatg
    14281 tggcaatgcg cagatcgaga agatcacgcc ttagcgtttc ggcgggatat ggagatgtag
    14341 gacacggctg gttattccgg ccgcgtatcg ggcctcaggg cagtggagca tgaatgggag
    14401 ctcgagcgga cgctggaagc caatgccgct tcagcgcctt ggtgggactc ggccccggcg
    14461 ttcgtggctc ggcggttttt cctgcttccc gctgcggggc tggtgtccgc cgatccaggt
    14521 gttccgacat ctgggggtgt ggacggggaa atcgatgtcg aacggtgcgc tgaaaaccat
    14581 tcgcggcgag gtcgacgatc tgccgtcgaa ggtacgcggc agcgttgccg atgccggtgt
    14641 tgagcgagtg tttcggacag tctcgaggtg agaccgggtc ggccccaccg cttccttcat
    14701 cgaacgcgca tcgtcgccga tcgccaacgc atcgcgtcca ggccggctct cacaccaccg
    14761 gctccgtccg agaacgcggg atcagccggt actatttcct tccgggcacg agaggctcgg
    14821 gctgatccgg ccggcgtccc atttccaata gaggcaaaag tcggtgcggt gcggctcgtt
    14881 aaaaatgtcc cggtaacaca cccggccgtg aaaatagatt gcggattttc cgcccttgat
    14941 ggcatcgacc tccgccgccg tgagcgggct ttcccgcgcg attcccgggc gcttgggcgt
    15001 tccgataaac cacttccccg ccttcggatc cggcgtgacg tggcgacact cgtcgttgac
    15061 gagccgacgc gtcagagggt aatccatcac aagaattctt gaaaacgacc cgttgtcgta
    15121 cgcgggagtc ctgccgatat tgtggaaggt gtcgtggacg cgaggcacca ggccttcctc
    15181 gatcgtactg attccccggg gccgcacatc gatgtaggcg cgcaattgtc gtttcgccgc
    15241 ctcttgaacg atatagtttt cccaactggg ataaccggtc gcaaaggccg caacaatcgc
    15301 agcgatcacc acgctccaaa aaatcgtcag tttcagccgg aattgcttgt tttcggcgtc
    15361 ggagagtaac tggggtggcg gcaggccgtg acttccgtct ccctgagccg aacgggcttg
    15421 ggcttgcgga gatagaccgg cctggtttgc ggtatcggtc cggtctgttt cgtcttgact
    15481 cgccatggag tactccccct tgtggttcgt accaggaagg ctcgtcacac agctcatacg
    15541 cggagcgctt atgtctccac tataggaaag tggaaggatt aaggcaacag gaagaaggtc
    15601 aaggcctgga ggnnnnnnnn nntgcgcaag tctgaaggat caactagcct ggattattca
    15661 tgaatgccgt cccgaggcgc tcccggcgcg cgcgtgtgag tgtgtgagta cgggtgaaac
    15721 tgttcgcctg ctccaggtgt gagagaggag aagtgctgtg aatgctacag agcagaccct
    15781 gccgacgaag gctgatgact atcttcgcat gcgggctttg gcaaccgggc tcctgttgtt
    15841 catggcgctg ctgtttgtcg tggccttgtt tcaacaagag acgcatccca tgtttgcatg
    15901 gctccgagcc ttcgctgagg ccgcgatggt gggcgcgctt gccgactggt atgcggtgac
    15961 ggcgctgttc agacaccccc tcggcttgcc gattccgcac acggccatca tctccgggaa
    16021 taccgagcgc attgcctcta atatcggcag tctcacccag cggaagctgg tcacgccgga
    16081 ggggatcgcc cgattggtcg gttcctggcg catccctgaa gaactgatcg aggcgctcct
    16141 cgcccccgaa cgcaggcgtg cgctgacata cgaatgcgcg cagatactcg ttcgagcgct
    16201 gaacgcgtcg gaagatgcga cgatgcagag gatgctgcgc aacattgcca ccaaggtcat
    16261 gcgtggcgtg agcgtcgccc cccttgccgg tcagatgctg gccggctttc tcgggagcca
    16321 ccaacgggat cgcctcctga acgacatcct gtctgctgtg ctcgagtacg tggacgccaa
    16381 cagggactcg ttgggcagga cggtggctga aaaacttccc tggtcgcgcg tcctcagttt
    16441 cgtgaagctg gatgacaaga tctcccataa agttctggat tctgtctacg acaccctctg
    16501 cacgatgcgg gacgatccgc acgatccgat gaggcggaag gcgattgagc ggctccacga
    16561 attgtctcag tggctcatgc attccggcga agcgttgagg cgagaggcgt ccctcaaaga
    16621 aaagctgctc gcatacgaca cgttgttgca gttccttgac gattcatggc atcggctcaa
    16681 gcaatggatg ctcgatgatt tgagccggga tccctccgac attcggacct atctcgatgc
    16741 cgccctggcc gatatcgggc gcacgttgca gaaagatgcc gcattgcgcg ccatgttgca
    16801 tgatggggtg cagggtctgg tcgaggcact cgccaccagg cacagcgaca agatcgggga
    16861 gttggtggcc aacacggtca gggaatggtc gccggctcag atggtggaga ccatcgagcg
    16921 ggaagtcggc aaagacctgc agtacattcg catcaacggg acgatcgtcg gaggcctggt
    16981 gggattgctc ctgcatgccc tggccctgtt gatcgggggg cggtaaaccg agggagcgtt
    17041 gccatcgcga agcgtgggcc tacattgtgg gccggcaggg aaggcggctt tttgtcgggt
    17101 gcgcatggcg cgagatgtga gaggtccaga agtgggtggg agagattacg ctaaggtgta
    17161 gcggccggct ttctgggtgc cgctgcgttg caccagccct gcttcgagga gcgggttcaa
    17221 tagatccatg gctccttgct tggagacccg caagctctcg cgaatctcct tgggggtcat
    17281 tgaccggcgg tccttcaaca actgcagtaa ttgctcttgc cgtggtcgca acatcacctt
    17341 cttcggtgcg gcgctcgctc cgagacgctg aatgcgcgtc cagactcgat ccagggttac
    17401 ccgcaaggcc tgggcacaat actcgatcca ttgggtgagg tcatctccct gttttcgcac
    17461 gttcgccaac gccgcataat aggcagggcg atcctcgctg tacacttcgt cgacagagaa
    17521 gatatggtgc atatcgaatc ctcgtcggta gagttcccac aaagccaagg ctcgccccgt
    17581 gcgcccgttg ccgtcagcga acggatgaat ggcttcaaat tgatagtgga gaatggctga
    17641 ggagatcacc ggagaccact cacgtgattg tccgttccac catgccagga gctccgccat
    17701 caactcgcgg accagttgcg gctcgggcgg tgtatagcgg cccacataga cctggatatc
    17761 gcggtaccgg cccgcgtcgc cttgatccat gacttcggcg gcgagtgtcg tgtggagggt
    17821 gaaaacatct cgatgagtca cgcgtttctt gtcggcatgg cgctcaacat accgcagtcc
    17881 tgcgagataa ttcaggactt ctcgtttggc acgagagggt tcggcaggaa ggccgtgtcc
    17941 ttcttcgatg agccggactt gttccagcgt caatggattg ccttcaatcg ctgtggagct
    18001 atgggtgttc cgcacgcgtg cgtcgcgttg gaggctgggc atccagggaa cctgaacggt
    18061 ggccgacaaa attctttccc gtaatacggc gattctttcg agttcgccga cgacttgggc
    18121 ggagacggca aattttggtt ggtacggcat cgggcacagg gtaagtgatg ggtcaagtat
    18181 gagtcaagtt atgagtcaac cgcgaacccg gagaggaatt gtgacgctgg agcatagggc
    18241 gtaaggctgg cggcaggaga ggaggaaatg cccgttcatt ccgcctgatg gcctcgcgga
    18301 tattctggag gtgctgcagc tttggagcag cctcaaggtg gccttgcgct cattgtctgc
    18361 gctcatcaga aaatctgggg ctccgtggga tgagtggtat cggacgggcg aggaattgtg
    18421 gccggttcgg cggcaggcgc gacatgcccg gcatcgagca ggatggcgac gagcatgtcg
    18481 ctcatgacgt tcacgccgga gcgggcgcgg gcgatgatcc aatccacggt catgatgagt
    18541 ggaatggccg caatgatgac gtgatccggc aggcccgcgg cggctagcac cagcggcagg
    18601 acaatcagtc cggcttcggg aatgcccgcc acaccggccc ccgcaatgat ggaggcgagc
    18661 acgatcagca cctgtttcac catcggcagg tcgaagccca gcgcttgcgc gagaaagagg
    18721 gcggccatgg cttcatagag ggtgatgccg tcgttgttga ggttcgtacc gacgcaggct
    18781 gcgaggcgcg acgattgcgg cgagacgttc atgcgatgga ggcactggag tgtcaccggc
    18841 acggtcgcga ggctgctgtt gcaggacacg gcggtcagga tggcgtcggc cccctggccc
    18901 aggtagacct ttggcgactt ctttcccacc agccaggcga ggagcggata atagatgagc
    18961 gcgtgggccg cgagtcctgc gagcatggcg acgaggaaga tccagagcac ggaaaagacg
    19021 ccgatgccgg ccttgcccac gacctgcgcg acgacgccga agacggcgag cggcaccacc
    19081 aggatgatcc attcaagtat ctgcaccagc cagacataga cccgttcgat gccgcgcaca
    19141 agggcattga ccagcccggt ggtctggtcc ttttcgctcc ggaccatccg cagggtgacg
    19201 ccgagcacca gcgcgaggag gacgacgccg atgatgttgt tgctcgagaa cgggtcggtg
    19261 atggtgcggg gaatgtagga ggcgaggtac tcgatcgggc tttgagaccc ggcctgcacg
    19321 ttggccagca cggttgcgga gggcttggtc cccggtacca gttggagcag ggcatcgacg
    19381 cggccctgcc aggccaggcc cggctgccag gtgttcatga tcgcgaggcc gatggccatg
    19441 gcgacggaga cgttcacgag gcagatcagc aggagtttac ccccctggcg cagcggaatg
    19501 gtggtgcgga tgagcgcatc gaggatcgcg aaaaagatca acggaatggc gagtgtcttg
    19561 agcagggtga cgacgaagag gccgagtttg cccagctgcg cattgcgcag accaccccaa
    19621 tagggctcct gtccgaagag ggcgcccagc agcccgccgc agatcacggc aatgagcact
    19681 tgtgtgtaga gcggcagtgg gatccagcga tgcgtcggct tcatgtcgat ctctagtgga
    19741 ggcggaagaa taccggtttc ttcactggat aacatcccgc ttgagggaaa ggctagcccg
    19801 gactcttcag gaggacctcc tcagtcgttt gctcctctcg aaccgcgggc ttcagtcttg
    19861 aattcctcgg taggacactg gtgaacaatc ctggctgggc cctaagggag tgcgtccgaa
    19921 acccggcgca ggagatcagc gccagcactg cccattgcga ggaccttgtg aaaggagtat
    19981 tttcttaagc attctcacct cacagaagga ggctattatg agacgcttcc gactcctctc
    20041 cttgaccctc gggctgcttc tttcctgtac atcgccggcc ttgtcggaac tgctggccct
    20101 gctcaactac gagagtaagc cagaccaatc ggtcagacgg gaagggatcg cgattatgga
    20161 catcgatccc gagtcgtcgg acttcggaaa ggtcttgatg gagattcccc ttccgccgga
    20221 ccttgtcgcg caccacattt tcttcaaccg cgaccggacg aaagcctaca tcacggcctt
    20281 aggcaaaagc gtcctccacg tcgtggacct cacccgcttt ccctatcgcc tccgcgccat
    20341 cgatgtgccc gactgccagg tgttggaaga tctcgtggtg tcggaggata accggatctg
    20401 gtacctgacc tgtatggggt cgagcgccgt catcatgggt gatgctgtga aggacaagcc
    20461 ggtcaagacc attcgtacgc cggacggcgg gggcgcatcc gtgctctatc cgcacggcat
    20521 tgcgattcac aacgggatcg accgtgtgct gattacgagc acggtgaagc ccgatatgtc
    20581 ggatgcgggc gactcggtga cggtgttgca ggcgagtacg ggtgcggtgc tctcgacgca
    20641 caaagtgtcc tctaaacctt caccggccaa ggctgccccg gtcgaagtga tgtttgcgcc
    20701 caactcgaac cccccggtgg tgcagatcac aaacatgttg gagggaacct tgtggaccgg
    20761 cacctgggat gcggcgagcc agtcgttttc gtttcagcag gtggacgact acgggccccg
    20821 gcaacagggc atgccgctgg agatgctcta caatgcgaag ggggatcgat tgttcgtcac
    20881 gacggcgaag ccgggatttg tgaacctgta cgacaattcc gaccccagtc acccgcggtt
    20941 tctccaagcc atccccgcgg ctcagggcgc ccaccatagc gtgctgtcgc ccgatgaacg
    21001 ctacctcttt gtccaaaaca gcttgctcaa cctggaaggc atgagcgacg ggtccatcac
    21061 cgtgattgat ctgtccaaag gcgggacggt gctggggagt atcgacacgt tgaaggcgca
    21121 agggttcaac ccgaactgca tcatgctgct gcccaatcat ttccggcagg ccaacttgcg
    21181 cgcaagcctc gttgcaaagt gaaacggtgt gagggcacaa agcggcagac gtgacagcga
    21241 aggcggatgt tcagtgcgaa attggaggaa actccagtat ccgggtgccc ggctacgccc
    21301 gcttcccttc ttgactccca ttttcaccgg ctcctataat tcggcggttc acaagcggca
    21361 accagctgat ttggcgtaga gaatggccga gtttacgcat ttcaacgagt cggggcgggc
    21421 acggatggtc gatgtcgggg cgaaggcctc caccgagcgt ctggcgacgg cgcaagccac
    21481 ggtattcctt cagctcgaga ccctcgaaaa gatccagcag gggaagattg ccaaggggga
    21541 tgtgctggcc gtcgcccagg tggccggggt gatgggggcg aagaagaccc cggatttgat
    21601 ccccatgtgc catcccatct ttttgaccag cgtcgatatt tctttcaccg aaacagctga
    21661 acccaaccag gacgggcgtt gcgcgatcac gatcaccgcc acggccaaga ccaccggtcc
    21721 gaccggcgtg gaaatggaag ccatgaccgc cgcttcggtg gccgcgttga cgatctacga
    21781 tatgtgcaag gcggtggacc gggggatgag cttcggcgac atctgcctgt tgacgaaatc
    21841 cggcgggaag tccggcctct ataccaggaa ggtcggctga gatggtcacg atccaacttt
    21901 tcggtatgac caagatgctg gccggtaacc aagggacgct gtctctgtcg ctgcacgacg
    21961 ggaaacgggt gaaggacctg gtgtcggtga tcgatgcggc ctatccgaag atcggcgagt
    22021 tgttgcagaa gaaaaaggtg ctggtgtcgg tcaatcaaga gatcgcacac gaggatctcg
    22081 aagtcaaaga tggggacgaa attgccctgt tgcctccctt tgcgggcgga agccaaggat
    22141 gctgaaaaaa cctgtcagca gcgttctcgc ttcgcgcaga aactcgacgt acagaagccg
    22201 tacgcctcat ttccgtgctc gctgcggcct tgctgagcag gctttttgag catccttagg
    22261 gatgcgcgtc actgggcaga ctagaagagg accgaacaat gagtctcgaa caggccacca
    22321 aagccgacca gcccgccacc gacgatgacg cgatgctggt ccgcgtccaa cgggaagact
    22381 tttcgatcga cgaggaactc aagcgggtac gccggcgctc caaacggatc ggtggcattg
    22441 ccatgtttct gggcacagcc cgtgaccgct cgaaggggaa ggatgtggat ggcatcacct
    22501 tcgagcatta cgagggcatg gcgcagaaga aactccgcga gattcgtgag cgtgccatca
    22561 aggatttcgg cgtgatcgaa gtgctggtcc tgcaccggta cggtgagatc accatcggag
    22621 agaatattgt gctgatcatt gccgcggctg agcatcgcgc cgaggccttc cgtgcctgtc
    22681 agtgggcgat cgacgaactg aagcaaatta cgccgatttg gaaactcgag cacacccccg
    22741 aaggagaggt gtgggtcgag gagcacccat agccctgtca agggcgagca gtcagggggg
    22801 gagaggcccg cgccttcgag tcactatgga ccaccgcacg ctcgatacac ccgctcccac
    22861 aattttcgac cgcttagggc gcccgctccg tagtctgcgc ctgtctgtga ccgaccgctg
    22921 caaccttcgg tgcaagtatt gtatgcctga agacgactat gcctggttgc cgcgcgatac
    22981 catcctgacc tttgaagaga tggccgaact cacggcgatt ttcacggagc tgggggtgga
    23041 caaggtccgg cttacgggcg gggagccgct gttgcgtcgg gacctgccgc ggtttgtccg
    23101 gcagctgtcc gagaatcgcc ggattacgga gatcgccctc accagcaacg gtgtgctcat
    23161 ggcggaccag gctgccgacc tgtcgtttgc cgggttgaat cgcgtcacga tcagtctgga
    23221 taccctgcgg gccgaccggt tccgcacgtt gaccaagcgt gatcttcatc atcaggtgtt
    23281 cgacggtatc aaggcggtgg tgcaggccgg gtttccttcg ctgaaattcg acacggtcat
    23341 catcaaaggg tacaacgacg acgaactgat cgatctgatc gagtacggaa aaacagtcgg
    23401 cggcgaggtc cggttcattg agtacatgga tgtgggaggg gccacggatt ggtccatgaa
    23461 tcaggtactt tcgcgggcgg agatgctgga gcacatcggc cgacactatg gcgaagtcac
    23521 acccatcgtc gagaacagtg ttgcgccggc ggagcggttc cggttaccgg acggcaccag
    23581 cttcggcatc attccgtcca ccaccacgcc cttttgtcgc tcctgcgatc ggagccgcct
    23641 cacagcggac ggcatgtggt atctctgtct gtatgcgaag gacggcctcg acctgcgggc
    23701 gccgttgcgc cagggccgct cccgcgacga gatcaaatcg ttgatcaccg ctgcctggga
    23761 agggcgggcg gatcggggtg ccgaagagcg caaggctctc gaagcgctcg gcctccggga
    23821 acaacgactc atcgaaatca accgtctacg cgaagatccc cacctggaaa tgcatgcgcg
    23881 agggggctga ccgtcagttc atgctgttcc tgttaccggg gcagtcgagg cacttctccc
    23941 ggagcagcgg atgttcgatc ctcataccct gaccatcttg agtctcggat tcgtgttggg
    24001 gcttcgtcac gctctggacg cggatcatct tgccgcgctc tccacggtat tggcggagcg
    24061 tccgaccgtg caggcttcca cggccatcgg gtttttttgg gggctcgggc acacgctgat
    24121 gttgctgtgt gtcgggaccc tgctgctggc cttgaacctc acaattcctg aaaccctggc
    24181 cagcctgttc gagtttgccg tcggggtcat gctggtggtg ctcggcctct cgctggcacg
    24241 ccggatctac cgggaacaat ggcacctcca tacgcatgaa cacgatgggc agccgcacgt
    24301 gcatctacat agccatcatt tgcagccgga tcacgcgcac ggacattggt atcagggatc
    24361 gcttcggcca ctcttgatcg ggatggctca cggactggca ggatcggcgg cgctgatgtt
    24421 gatcgtgctc tcaaccgtca cggggatcgg tcaaggcatc ggctatatcg tggtgttcgg
    24481 cgtggggtct attctcggca tggtcggcgt cggggcgata ctgagtctgc cggtggtcta
    24541 ttctgtcacg gttggtccgc gcgcctattg gatggtgcag ggcctggctt gtctggcaag
    24601 tattggcttg ggattgttga tgatggtccg gatcgggctg ggcggcggtt ggtcctagcc
    24661 gatctgtgaa tgccgacgag ctgtcgcatt tgagaacaga ttggggatgt acccttcccg
    24721 cggcgagggg gctcatggaa ccgaccgtgc aaatctgtgt gcgccttttc gagaggtatg
    24781 ttattctgcc ccgcagtgcg gaggacacat ccacaagctt gtgatggagc gccggagttc
    24841 gagggacgtt ccgggaatcc acagccatga atgtccgcgt gctgggctgt catggctccg
    24901 ggcagctcat tccgggggag aacggtccga tccagtgcgg tacctgcggt tttctggtca
    24961 atgaccagtt gctcgtggat gccggcacga tcgggtcgcg cctgttcctc gatgagcaac
    25021 ggcggattcg ggtggtcctg ctgacccatc tgcatttcga tcatattcgt gaattgccga
    25081 cgctggccga taatctggtc ggcgagatcg atgagccggt ggtcatcgcg gccattcccg
    25141 aagtgttgga cgggctacgc gcgcatattt ttaacggaac ggtgtaccct gatttcttcc
    25201 ggctgcccga tcccgccagg ccggtgttcg tcccttacga gttgcgagcg ggacgagagg
    25261 atctgctgtg cgggatgggc gtgacgccga ttcgcgtcaa ccatgtcgtg ccgaccgtgg
    25321 gatttctcct tcgcgaaggg gcgcacaggg tgctctatag cggtgatacg tatcaaacgg
    25381 acgcgctctg gcaggtggcg aaggaagtca gcgggttgcg ggcggcgttt atcgagagtt
    25441 cgtttcccaa tgagatggat gagttggcgc gtgtggcgaa acacctgact ccggcgctgc
    25501 tggcacagga atttgccaag atggcgaggc cggagttgcc ggtctacgcc taccatctca
    25561 agccgcggtt tcgcgaccag atccggcagg agctcgggca actgggtatt cctcatttga
    25621 ccgcgcttga agagggtcaa acccttgtac tataggtgag cacagatggc atggtttaaa
    25681 aaaggcaaaa ccgaggaggc agagcctccc aaacgctcca aagtggcgga ggggatgtgg
    25741 ctgaagtgta atcactgccg ggagatcgtg tatcgcaaag aggtggaccg gaacaacaag
    25801 gtctgtccga agtgcgacta tcactttccc atttcggtga ttgaacgtat caacctgttg
    25861 gtggatctcg gaaccttcaa agagtgggac gccgagctgg agccgcaaga cccgctccag
    25921 tttcaggata cccgcacgta taaagaccgg atcaaggcgc aacaggaaaa gaccggccgt
    25981 aaagacgcca tggtcatcgg ccagggggcg atcaacgggc gcaaggtggc gctgtgcgtc
    26041 ttcgatttcg ggttcatggg cggcagtatg gggtcggtcg tcggtgaaaa gatctgccgc
    26101 gccgtcgatc gggccttgga agggcgcatg ccgttgatcc tcgtgacggc ttcgggcggc
    26161 gcccgcatgc aggagggcat cctgtcgctc atgcagatgg ctaaaacgtc cgcagcggtg
    26221 gcgaagttgg gagaggcgaa ggtgccgttc atctccatct tggcggaccc gacgttcggc
    26281 ggagtgaccg cgagcattgc gatgctggga gacgtcatta ttgccgagcc gaaggcgctc
    26341 atcgggtttg ccggtccgcg cgtcatcgag caaaccatca aacagcaatt gccggatcag
    26401 tttcaacggg ccgagttctt gctggatcac ggcatgatcg acatgatcgt cgaacggaaa
    26461 cagttgaagg aagcggtcag tacactggtc ggccacttct agcaggatgc ggaaaacgtc
    26521 cgccagcggc gttctcgcac tccgtgaagc ctgaatcgtg aagcgtatct cgttgcgggt
    26581 aagatcgtat gctgagagca aagatgccca ctctcgatgg ctggccatac gccatgcgct
    26641 ctatgccata ggctcttttc tgcggacgag agacgaacga cgcttcacga acgacggacg
    26701 gtggggacgg cctttttgcg catcctgctg gcaattccaa tttcaacatg ctacgtgacc
    26761 tgatcgtggt gtctggtgca aaagtggatt ttcccgcagt cttctagcgc aggcgagttc
    26821 tcacgcatga ccacgatgac ttactccgcc actgtccaat ttctctacgg cctgcagcag
    26881 cacggcatca agttgggact ggagacgatc cgtgccctgc ttaccagggt gggggagccg
    26941 catcgttgtt atccggtgct ccacatcggc gggacgaacg ggaaaggttc taccgcggcg
    27001 atgactgcgg ccatcttgca ggctgccgga tatcgagtgg ggctctacac gtcgccgcat
    27061 ctgatcgact tccgtgaacg tatccgtatc aacggtgtga tgattccgga ggacactgtc
    27121 agtagactgg tcgagacctt acggggtgcc gctgcgcccg atcttgcgcc gacttttttt
    27181 gagttcacga cggcactggc gttccagtat tttgcggact gtcaggtgga tgtggcggtg
    27241 cttgaagtcg ggttgggcgg acggttcgac gccaccaatg tggtggatcc gctggcctgc
    27301 gctattacga cgatcggctt ggaccatgag gcctatctcg ggtccacgtt agaggcgatc
    27361 gcgtttgaga aggcaggcat tgtgaaacca gccgtgccgg tcgtacttgg tcgcatcgag
    27421 tcctcgactc gccgggtcat tgaacagcgc gcgtcggagg tggcggctcc ggtgtatgtt
    27481 gtggattgcg agttttcctg cgatggtcct tcaacggctg attgccggta tgtcggcggg
    27541 tcttcgcact acgatcatct ctcctgcccg ctgaaggggc ggtttcaact cgacaatctc
    27601 gcctgcgccc tggccttgat cgaactcgcc cgcgagcgcg ggctagccgt gtctgagacg
    27661 gcggtgcgga gtggattgca gcatgtcgca tgggaaggcc ggttggaaat gatcggcgag
    27721 tcgccgacga tcatggtgga tggggcgcac aatcccgctg ctgcggctgt cttggccgag
    27781 tacctcgccg attggcgacg gaccaggcct gccgcgagga ttagcttgat tgtggggatg
    27841 atgcgcgata aacatccacg tgaattcctt gcgccgttgt tgtcccaagt ggatacgttg
    27901 atcctgacgc aggcggatct gccgcgtgca tccaccggtt cggaattgcg aacgctgctg
    27961 caagactgtg cgcccaccgc ccacgtggcg gccacctccg ccgatgcgct ggctgccgcg
    28021 aagcgatccg ccgctccgtc cgatctgatt tgtgtcacgg gctccttgat gctggtcggc
    28081 gagatcaagg cccttctccg agggtgttcc ctctcacctg ttcgtggttg agatggggcc
    28141 agaccgctac tcggcttcca tacggcggcg gacggctggt tgtccgttcc cggttgtcct
    28201 cctgactctc ctcctcagtg tgttgctgct ggtcgaagcc gcctcggcgc aatcaacggt
    28261 gaccaccgcc tcaccgagca accagcccct caccgtgacg gccgaacgca tcgaccatct
    28321 gcaggagcag gaggtgtacg aagcggacgg gtcggtggtg gtgacgcaag gctcgtttcg
    28381 actcacggcc gatcatgtca cgatcaattc gctgccgggt accttagtgg cgaccgggca
    28441 tgtgcatttc ttcgacccga gttccgatct gaagtcggag cggctggagt taaacgtgaa
    28501 taccgaagcc ggagtcatca cccatgggtc gatgtatgtc cggccaagca actcgttttt
    28561 taccggtcgc agcatccgga ggctgtccga ggatcactat cgcatcaagg aggggacgtt
    28621 cacgaattgt gacgccaaag acggcgaagt gccggcctgg cgattcaagt tcgacgatct
    28681 cgacgtgaac acgggcgaaa gtatcgggat gaagcgggcg tggctctgca tgcgggatac
    28741 gcccattatc cctgtgccga ccctgaccta tccgctcagt aatcgtcaca gcgggttcct
    28801 cattcccacg cccggctacg acaaccgctt cgggctgcac taccagcagg gctatttctg
    28861 ggccattaac cccagccagg acctgacgat cgcaccttcg tattattcgg atcttgggta
    28921 cggaagcgat tttacctatc gctattatct ggaccggcgg tcgagcgggc agtggtttgc
    28981 cagctttctg cagcagacga aactgccgaa tgtgtcgggc gtcgatcaga ccgcctcgga
    29041 tgagcggcgc ttgcgaggct tgatcagcgg ccagcatgtt caacaggtca cggacacact
    29101 cctggtgcga ggccaggcgt cgtttgtgtc ggatcggcag tatctccaac agttgagcaa
    29161 ctcaggagcg cagcgggcct ctcccagcgg cgaatccaca ttgctggcga cgcaacgcct
    29221 gccctacggc agcgcctatt ttctgggcca gtatctccag ccgttgaact ccggcggtcc
    29281 cgacacgttc caacgcctcc cggaggtcgg ctatgtgttg ccgaatacgt cggcgttcgg
    29341 gctgccgctg ctcatgaatc tcgacagtaa ctttgtgaat ttttatcgcg agcagggatt
    29401 cgcggtgaat cgcatggatt ttatgcctgg cgtcaccacc gatgtgatcg atgtcgggca
    29461 tgtgatcggg ttgacgccgc attttaaatt caaggaagtc tattatacgc gcggcattca
    29521 ggaggccagt cctctccatc gcgagacgtt ttgggcggct atggatgcgt cttccaggct
    29581 gagccgccgg tataccgggg gtgacggcgg gagtttcctg cacacgatcg aaccgagtgt
    29641 gatgtatgaa tacgtgccgg ggagtaacca gtctcagatc gcccagatcg atcaagtcga
    29701 tgacatcccg aagaaaaact tattaaccta ttcactccgg accaagttgc tcgaacaaca
    29761 ggtgaacggc caatcgttca actggctcga cttgaccctg gcgcagagtt atcacgtggg
    29821 cggggtgcag acacgcgcgc gagagtttac tcccggcgtg ttaccgttcc tgggatctct
    29881 cacgcagccc ttgcagccgg cgaccgtgga ggtgcagggg cgaaaactgt ccgacttgtg
    29941 gctacgggcg gtgatcggca ataccgcgcc ggaattcttt cgtccacccg gtgtcaacga
    30001 agcgctcgga aagaatgtga cgggcggaca gatgttacct ccgacgaatt cgtatctgac
    30061 ggtcgatgcc tttctcgatc cctaccgggg gacattcagc cagtggaata ccgatcttcg
    30121 ggtgcagcaa tccaactatt ggtacgtgga ggtcgggcaa cggttcagtc gtgacggcaa
    30181 ccgggtccgg cgcggcgata tttggaatcc ggtctcgttt aatgaggtct atgctcccac
    30241 cgaggagatc cagttcgtca ccgcgggcgg cggattccgc acaccctggg gctggaccgt
    30301 cggcgccaag ggctattacg atgtcaaagg cggccgcagt ccggaatacg atgtggtcgc
    30361 cttgtatcag aatccgtgca agtgctggtc gctcgggttg tactatctgc agttcccgga
    30421 ccgccagagt tactccttca tgctgagcat gaccggcatc ggctggacag agaacttcgg
    30481 cacgattgtg gcgcgcacga ttttgggtcc attgctggtc ggggatcgcg gcttgccctg
    30541 ggcagcgccg ggtggcccct atggacgaac cctgaatccg atgccggtgc agtcgatgcc
    30601 gatgggtcgg tattgagccg caaagtctcg cctgacgggt cgaaattaga cggcccggag
    30661 cgcgatgcgg agacggtgag ggaggcgcat cctcatgcga cgaaaccagc tcttcctgtt
    30721 gatcggggta tgcttgtgga gcgcggttca gggggcggtc ccggctcagt tggaggcggc
    30781 ggagcagcca tcgcatcttc aacgcgctgc ggtgtttctc aacgccgctg actaccgtca
    30841 agcggtggag gcctgtcagg cggcggtgcg ggagacgcct tcggctcgaa cctatgtctt
    30901 tcttacgtat gtgtatcacg cgctcgatgg ctaccttgaa cacctggcga ccaccgatca
    30961 atgggttcgc gtggaacagc tctacctgaa tctcgcgacc ggacggagtg aagatctgat
    31021 tgatccgccg gatgtgctcg cgcggatcgc aaaagaagtg atccagcagg cggtgcaacg
    31081 acagtctgat gtcaccgcgg cgatggcggc tcgcttggat gaggcgctgg tgaagcagtt
    31141 ctggcaggag caggatgcgt ggcgacgcgc taggccgacg gactggtggt ccggtgtacc
    31201 acctgagtgg cactgggata ggccgtgatt tgactctctg caaaaccgtg ttataggatg
    31261 acggacggtg tgtggaaccg cttcagcaca tcgaaaaacg atgatatggc aggtcgagcg
    31321 aaggaggaca ctgtggcagg cgatgcgttg aaggcgactg atgcgacgtg ggatgaggat
    31381 gtcatgaagg catccgaatt agtgatggtg gatttttggg cggtctggtg cgggccttgt
    31441 cagatggtcg cgccgatcgt cgatgaatta gcaacagaat atgcgggtaa ggtcaaggtc
    31501 cgtaagctca ataccgatga gaacccggaa attgccggac gctatcaggt gatgagcatt
    31561 ccgaccattc tcttcttcaa gaatggtcaa gcggtcgagc ggctggtggg ggcgcgtccc
    31621 aagcgccagt ttaaagaaat catcgattcg cttctcgcgc aacattccgg cgctgcctag
    31681 cgcctgatcc tcagcggcat gctgaccgag ctgcgcatct cgaatttcgg cgtgatcgag
    31741 cagctggccg tccgtttcgg ctccggcttc atcgtgttta ccggtgaaac cggagccggc
    31801 aaatctctcc tcattgatgc ggtgacgctc ctggtcgggg gccgtgcctc gacggataag
    31861 atccgtgccc agtccgatga agccgatctc gaagcggcgt ttgtgttgcc gtccgaccat
    31921 ccccttctcc atctgctcca gaccaaggaa ttcgtccgtc cgggtgagac ggacattgtt
    31981 atccgtcgcg tgatttccag gacgggccgc aaccggacct atctcaacgg caatctctgc
    32041 ccggttcatc ttctggagga actgggcggc gcgttggtgg atgtgcatgg ccagcacgag
    32101 cagcaatcgc tgttgtcctc cgcggctcag ttggaggcgt tggatgcctt cggccgactt
    32161 catgctcttc gtcatgacta tcaggtcgcg tatcggtcgt ggcaggagcg ggtggctgaa
    32221 cgtgagacac tgactgtgca catcgcccaa cgccgagaac gagaggatct gctgcgtttt
    32281 caatttcagg aaatctccga tgccgcagtg gaggcggggg aggacgcgcg gcttgaacag
    32341 gaacgcccgc gtctcatgca tagtcaacaa ctgggcgacc tctccgacca actccatgag
    32401 ttgctgtatg cgggtgatca gggcgtgctg agcctgttgg cttccgcgcg caaactgctc
    32461 gcgaagatgg tgtcgatcga tcaaactgcc gtcgaatgga cgcgggtagt agaggacgcc
    32521 atcgttccgc tgagggacct ggccgaccag atccggcact atcgtgatca agtcgaagcc
    32581 aacccggcac gattgatgga aatcgagcag cggctggatc gattgcatcg gctgagcagg
    32641 aaatacggcg gatcgttgga tgccatgctg gtgctacaag actctctacg cgctcagctg
    32701 gcccaactgg acacagcgga aacccggctg caggacctgg ccactgccgt ggaggatgct
    32761 gcacgtcgcg tgggtgaagt ggccgggcgt ctctctcgca agcgaatcga cgcggccaag
    32821 aaactcacga cgcaggtgac gaaagagttg agcgcgcttc gaatggagcg aacccgcttc
    32881 gcggtggagg tgcttccgct gcccggcgag acgtcctacg gccagatggg ccacgatgcc
    32941 gtggagttcg tcttttcggc caatccgggt gaaccactca agccgcttgc caaagtggct
    33001 tccggggggg aactgtcccg tgtcatgttg gcgttgaaaa ccattctggc cgagagcgat
    33061 cgagttcccg tgctgatttt tgacgaagtc gatgccggag tcggcggggc cgtggctgag
    33121 gtcatgggga cacgtctccg ggatctcagc cggcaccacc aggtgctctg tgtgacgcat
    33181 cttccccagg tcgggtccca agcccatgcg cattacctgg tagagaagca ggtgcgccag
    33241 cagcgcaccg tgacgcaagt ccggttgctg acggagcgcg agcgggaaga ggaagtggcg
    33301 cggatgttag ccggggtcac ggtgaccaac agtgcgcgcg cggcggcggc tgaaatgatc
    33361 ggcagcgcga aggaacgaca ggcgcgatcg gattaggtcg tcggtgaggt ttttgcgatc
    33421 gcgagggcgg gcagggaaga tttgcattcc cgcacgactt gggcaaaaag ctcggtcagc
    33481 ttcggatcga agtgagtgtc cgcttggagc acgatcttgc ggatggcgtc gtcgatggac
    33541 atcgggaggt gtcccggcat atcgaccgtc aggttgtcga aggcttgggc gatggcgacg
    33601 atgcgtgcca agagcggaat gccttcgccc tccagaccgt ggggattgcc cagtccgtcg
    33661 aatcgttcat ggtgataggc cacgatctgt cccacctcgg cctgaaattc aagcggtagc
    33721 agcatccgcg cgccggcctc agagtatgct ttggcgaggg tcccgttctt gatactgtcg
    33781 tcatcgttcg ctgcatactt atagttggga atactgattt tcccgatgtc gtggagaaac
    33841 gcgcccagcg cgagtccctt ctgatcggga agggaaaggt tgagctggtt ggcaagaagc
    33901 gaggcataaa agctgacgcg gctgcagtgg ttcaggagtt ggcggtcctt tgcttcgagg
    33961 aggtcggata acaacgggat caacgcagtg acatcgcctg tcttggcggc cggctcgggg
    34021 gcggatttgc cgatggagaa atagttggct tgcagatctt tccacgcctg ggccgcttcc
    34081 tgttccgttc cccacaaggc ggtcgatatt ttgagaaacg agcgcaggta gtccagccgc
    34141 tgctttttct cgagtgtttg gttgatgagg gtgatcagtt cggtcacgtt gaatggcttg
    34201 agtagatatc ccgccgcgcc gtgccggatg ccttccatcg cagacttcaa actgccatag
    34261 ccggtaatga tgatgacttc gatgtcggcg tagtcctgct tgatgtcttg cagcagatcg
    34321 attccctggc ggtcgggcag tttttgatcg agggtgatga ggtcgatatg ctggtctttg
    34381 aggacccgca gggctgattt ggcgttgtcg gcggagtgga tggtgaagaa cgggcgaagg
    34441 atgactttga gcgcgtcccg tggaccagtt tcatcatcaa cgaccagtac cgacggtttc
    34501 gtacctacgt attcaggtga tgtatgggtc cccatccaga gcgctcccgg gtgcattgca
    34561 ggcttcattg aaggacaaac gcaaaaccta taccgcccct gattgtgctg atcagtcaat
    34621 atatagcagg tcccgcatat tcaggccact agatatttga tgtaggggct atcgcctttg
    34681 atgaaatgag acagtctgcg gagcactgtt gcgccaacct gtgtcattcc tgagtcagtg
    34741 gaggctcggg attcaccccg ttatcgggcc ggctgatgcc caaggtatcc atgcggtact
    34801 tcagcatgcg gcgactgatc cccagcatgg cggccgcgtg ggtttgtacg taggtcgttc
    34861 gcttgagcgc gtccaggata atctcccgtt cgaactccat gacggccttt tcgagcgata
    34921 atcgtcccgc caacgtgtcg tcccggagcg aggtcgatcg tgtatcggtg cgtaggatat
    34981 tcggtaagtg ctccggcgtg atgacatcgg agcctttcga ccagatgaac gcctgctcga
    35041 tgatgttctc catctcgcgg acgtttccgg gccagggata gtgcgagatc aactccagcg
    35101 agcttttcgt aaattcctga ggaggacgtt tctcttcttc gatacgtttt gccagaaagt
    35161 gtttggccag gagggcgatg tcttcgcccc gctcgcgcag gggcggtaga tacagcgcga
    35221 tgacgttgat gcggtaatac aggtcctcgc gaaactggtt cttccggacc atctcatcga
    35281 ggtttttgtt ggtggccgcc acaattcgga catccacctt gatggactgc actccgccca
    35341 cgcgggtgaa ctcacgttcc tgtagcacgc ggaggagttt ggcctgggtc gccgcgctga
    35401 ggtccccgat ttcatcgaga aaaagcgtgc ccgtatgcgc caattcgaac tggcccacgc
    35461 gccgtgccgt ggcgtcggta aaggagcctt tttcgtggcc gaacagttcg ctttcgatca
    35521 gggtttccgg cagggccgcg caattgagcg ccacaaacgg gcgttcgcgt cgcgcgctgt
    35581 tgtaatgaat ggccttggcg acgagttctt ttccggtccc gctctcgccg gttacgagga
    35641 cggtggtgcg actgtcggcc acttgctcga tcttcgcata gatttcctgc atcgccgggc
    35701 ttttgccgat cagattgtga aaggcatacc gctggacgac ctgcgcgcgc agttgacgaa
    35761 cctcttgttc taattcctgt ttgcccagcg tgcgctcaat gacgatttgc agctcttcga
    35821 catcgaatgg cttggataga tagtcggcag cccccagttt catggcatcg acggccgtct
    35881 tgaccgattt ggtcccggtt agcatgatca ccgggacggc acgattctcg gtgcgcattg
    35941 tctggagcgc cgccagaccg tctgttcccg ggaggatcac atccagcagg accaggtccg
    36001 gcgcatcgtg tttgaaactc ttgaggcctt ccgcggcgtc cgccgcttcg agaatctcat
    36061 aggtcggttc cagcaccgtt ctcagcgagg cgcggacacg cggctcgtca tcgacaagta
    36121 gtacccgttt tttcatgtta ctcccgtgcg gctgaaactg tggtgcgtgc atccggtgct
    36181 ggaagattca cggaaaacgt cgaacctgcg ccgactcgac tctccaccaa tagcgtgccg
    36241 ccatgttcgc ggacgatttg atgcgcggtg gtcaggccaa ggccggaccc atcgctcgcg
    36301 ctattggtat gcttggttgt aaaaaacgga tcgaaaatat gctccaggtg gtccggggca
    36361 atgccgcatc cctcgtcctc gacctccagt tgtgcccagg ctgtgcggcc ggcttgcggc
    36421 agcacgcgcg tgcggatctg aatcacgccg ttgccgttgg gcatggcatc gagcgcattc
    36481 aatagcaggt tcaagagcac ctgtttgatc tgctggcgat cgagcgagag cgtgggcagt
    36541 tgaggggcca gcgtcgtgtg cagacgaatt cctcgatgcg aggcggtgga cgagacgaaa
    36601 ccgaggcagg aggtgaccag ttcgttcagg tccacgtcgc tcggctgggg agtcatgtaa
    36661 ctcgcatagt cgaggatttc gtgcagcagc cgttcgatgc gatggacgtc ttcaatggcg
    36721 acttggctga attcacggat gaacaccggg tcctgctggc gttgcggggc cagctgaata
    36781 aacgtcttga tcgacgtgag ggggttacgg atttcatgtg cgaacccgcc cgccataatt
    36841 tccaacgagc gcaatcgatc ggtgcgtctc atcaaggtgg aggagcgccg aaggtcgtcc
    36901 tgcgccaggt gatggtccaa ggtattgcag gcgatgcgtg cgacggtctc tgcaatgatc
    36961 acggtgtctt cgtccaggtg acactccgtg ccgatcggcc caagagcgca gagtccgaga
    37021 aactgcgtct tgttccggag aggaaggcac acggctgcct gtatcgatat catggctgag
    37081 ccgacgggcg aagatgaggc ttggtcgtat cggagcaggt gtagccgatc ggaaaggccc
    37141 gtgataaggg cgtggttatc cggcaggcta gccggcaatg ccgcaagcgt gcggtgtcct
    37201 agagaggcaa ggagatggta ttcgccgttt gcttctcgta tccatatggc ggctttcggc
    37261 aagcgcaact gttcgctgag gatcctcaag atgcactcgc cgacctgcgt gaggtcggtg
    37321 gcctgtccca ccgattcggc aaacgcgccg atcattctga tgggggcttc cgaagcagaa
    37381 agaatagggg agtgccgaat gtgctcgtgg agtgccatag tcttgcgcag aaaatatcaa
    37441 cccggacacc atcgtgcatg tggtgtcgtc cgacgcgccg catgtcgtgt gttagtgact
    37501 gaagtatata tgcgaggtat tgtttcagcc agcggaatca ggtttttcag cgagggcgac
    37561 cagaatctgt ccggagagcg tacgcacgag tcttgccgga gtagatgtga cttgggcgag
    37621 ggcggcgctc aggctctctt cctggaatgc gtgtagttga ccgacgcgca gggcgacgca
    37681 acactgagcc atgcgattga gggcaaggcg ggcgtctccc gagaaggcat cgacgccgag
    37741 ctcctgcaag gatgaacgat aggggagggc catatctgcc aagggggtga tgacgctcac
    37801 gatgagtttt ccgccggggc gaagcacacg gaacagttca cgaagagcat ggagaggaga
    37861 acgtgaaaag ctgagggaga gattcgccac gatgcgatcg aacgaatggt ccgcgaaggg
    37921 aagcgtttct acggatcgac cacgtaccca ctgctgactg acgggtgtcg gcccggagat
    37981 gcgaccggag aacagccgat ccacgtgctt cagggcgtca ctcgtcgcgt cctgcgcggc
    38041 atgcagcgcg gccactgaga agtctatgcc gatgtatcga agaggctggt tctggttcca
    38101 ggtttgagcg cggagacggt aggagaggtt caagagcagg agtctggcga atgaatgaat
    38161 gccgcagccg acatcgagca gggccaggcc tccatcgagc ggttggagga gttgatacag
    38221 ctgatcgata tattgccaat gcgcggggag ttcatccaaa cttcgtgtga ggtcggtctg
    38281 tgcggcccag aggcgctcgc ggcctgccgt tccggcggca tatttgatgc ggagtcggtc
    38341 gcgttcgagt cgtgcgcgaa tggatatcat gcgtgagaga ttcgagggag ccggggcggt
    38401 gggatacggg aagctatccg gagtaaggca gagctgtgcg attcggtgcc agcttgcgtg
    38461 cagtgccgat ggtgcgacag cgtcggcagg cagaagcgga gcttgtagca tgacgcgact
    38521 acctcttgat ccaagccgat gcagggcatc ctccacgagt gccggcgccg gtgagggagt
    38581 cgggtattca gcagcgcctg ggccggtcgt cagaaacacg acctcggttc cacaatgcga
    38641 gagatcctcc tgaagcgcgt tgctctctgt atattgagcg gccaccgcat cggccacgaa
    38701 atgatcgatg tcgagtggaa tccccaagag gttgccgagc ccgagccgcg ttccggccag
    38761 atgttcacct accagatccc gctgatggag gcttgtcagg cagtttcgga tgtcgagtgt
    38821 cggattgaga agtaagagcc ggcggactga cctgtgccaa tcctggcggc gtagcgcaat
    38881 gcggccgagc aggtccggtg ccagcaacgt gagggggagt ccggggaact ctttctgcgc
    38941 aaagatcagt atggtatcga gatcatcttc cagggatgtg agcgtggttt gcgtggggtc
    39001 gccgtcgctc aatccgatgt gacggctgtg atcatagcgc aggacttgca ggccgcatgc
    39061 agcaagtgtg taggcgaggg cgacataagc ctgttgtgtc gtcccatacc ccgagcagag
    39121 cagtaccagc gggcattgtg tgccgtctac tcgtctcgga acatcgtgac acagtgcaat
    39181 cgtgaggccc tcacgattgc gtaggtggtg tgaaacggtg aggaccggcg agtctgacat
    39241 cggttcggca agatcttgcg aagtgccgat ccgcggcgag atgagggccc tgagtctctg
    39301 ctcctgcgcg ggcgataaac cctgaaagcg cacaccgaag cggcgtcgga ctccttccgg
    39361 cggcgcggga ttcgaggttg tcctgacctg agattgatgt gtccagacaa tccgcgcagg
    39421 ccaagggttt tcagacccct caggcaaggc ggcgccgatt gacgaagtgt ttggctcgac
    39481 gggcgccagg tgaaggatct gctgattttc aggaaagtcg gatcctcccg gtaattcgat
    39541 gcaggcccca tccaggctca ggttgaccgt cactccgaga ggacgggttg gcgtggctga
    39601 tgagccgacc agtcgaatcg ggcatgggac gttgagacgt gcggcctcac gtctatctgt
    39661 cggcgctgcg gtggagggga ggggggcgtc ggggttgttc gcggcggttg tgaaaagagg
    39721 aaggatcaat ccttcgaacg tgacgatagt tgaaccgtct tgcagcccgt cgagaagtga
    39781 acggaggaca ttgcggtcgc gctcgccgct cggtgaaaag gaaatcacaa agttcttgat
    39841 ggcagggaga ggcaggtccg taagtgcagc cggtcgttca tgcacgactc cttgaagttc
    39901 caggaagctc accggtgtct tgatcaccac gtaggctggt tgcagatgaa tggaggctga
    39961 caggtcatgg atggtgagga gggcaccgcc ggtgctcatg tttcgtaaga cgcctcgaag
    40021 ggtggtgttt ccgacggata gttgcacggg gagcgcgaac agcttgcgtt cggaacgccg
    40081 tcggtcgggt cgttctccgg cgacgtgctg agattgaact cgaacaatgc cgggtttggc
    40141 aggcgaggct gtgggagatt cagcgccctc atccgcgaag aaatgagtgg gaacaggaat
    40201 tcgtaccgtg atggtcagac cgctgtcggg tgcatgttct atttcgatgg cgccaccatg
    40261 tgcctggaca atttggtgag cccgaaagaa ctgctcggga gaaatatgga aatcgggcgg
    40321 ttcgcccatg ggagcagcgg tgtgtttgga gctttccttg atggtgaagc tcacatactc
    40381 gcgcgagacg ctcgaataca gcggaaggtt gcgtgaagcg agtgaaggag tgactccggc
    40441 cagcagcgcc gtctgtagca gaagatggga cggagcgttg ggcaaggacg tgtattggtg
    40501 tgcataatta aggaggattc tcacgagatc ctccagcgcg tccggtgagc ccagaatgag
    40561 tggaaggtgt tgagccaagt gcgtggtcac gaggcgcccc gagccttctg tctcgtgccg
    40621 gatcgtttgt accacggtct caagaacatg gtgcagatcg agggggaaaa actctaaagg
    40681 aggggcatgg atggcttgag ccagttggtg gccaagttgg ctggcctgcg acgctttctc
    40741 gatcatcgtt tccacatgct gctcgatctt gctgtgctga attgagctga gcaatcgttg
    40801 tgaatcctgc aatagcgtgg tgagaggctg ttggagctgg cgggataccc gggccgcctc
    40861 ccgggaaagc gtgagttgac gggtggtttc ctggaggcgt gactccaact cggtgagtcg
    40921 gtcctgttgc gctttctgtt gggaaaggtc gcgcaggacc ccgaccgtgc cgagataacg
    40981 attggccgaa tcgaagagtc ccttggctgt tacatcgacg gtgatcgatg cgggtggtgc
    41041 atctggtagg gctttccgat gaagagtgag ctcaagtctg cgtaccgagc ggctgccggc
    41101 ccgtcgttcg ttcaggcgaa agcgtcctgt tgcctcttgg aggggaggga gtagaagcga
    41161 atagtgtcgt ccggcaagct cctcgggagt atagccgagc atggcggtga cggcagtacc
    41221 cacgtagaca aagcgaccgt cccgatcaag ctcgtacacc aagtcgctca gcgtttcgat
    41281 gaaccgctgg tgccgttgaa aggtgtggtc cagtttcatt tggaggtcgc gcttttcaac
    41341 cgcctcctgg acggaaaaca acaactcggt gataaaggcg ggtgaccgtt tgaacaggag
    41401 gaagtcagcg ccgttttgca gcgcctggac ggcggtttgg gaatcgcttt cattcgtttg
    41461 gaggatgatg gccgcatagg gagcattgcg acgaaggcgg gaaagaatgt cgagaccgcg
    41521 ttcagggcgt aaatcgtgat cgatgaggat gatgtgccat tcgccctgat ggctgaatgt
    41581 cagggcttct tcggatgagt agcccgcttc gatccggcag ccgggaaaaa atcctctcag
    41641 gctggaggtc accagcttga tttcatccgg ttgttcgttg atgaggagaa tgttgaggga
    41701 cggttcagcc ggcgacatgg aagatttcat ggttcctatg cagtggtgcg atcgttctgc
    41761 ttcggtgccg ccgggcgggc gcctgggaat gcaccactgc acagtgatga acatgcgctc
    41821 gctgtcagag ggacaattgt gtccccaggt agtcggttgg agtgcatgat tggttcaata
    41881 tcatacacct tattgagaat cccagcccgc tgcctgaggg ggcgcgttta cttcagtagg
    41941 ggagggtgct gtgcggagca agcaaaagtg atcacacccc aagaataagg tgcaattctc
    42001 gttgaggagg gggttcgggg cgagggtttc gccggtcgga tgatgtgccg gcagcgtcgg
    42061 gaacgaacgg gatgcccacc gcaatcgttg gaccgcggtg ggcggagaac tctcctatgc
    42121 ctagcgagct gaagctgtgt tggcagattg cgggcaaacg ccaggggtca tatacagcag
    42181 gtaatttccc attccatgct gaatattcgg gttttgaagc gggtggtggt gaaccatcac
    42241 catttcatag tcgtcgcgtt tggttaccgt gaaccctttg tcatcccggt acacctgatg
    42301 agggcgaaag tcacgaaacg tgccgtcggc acccacttca gggaccgttc gcaggagggt
    42361 ttctcgggag gatttattct ccagggcaac catcagcaat tcatcgtggc catgggggta
    42421 ggcgaatttg acgcagccgt ccatgttgaa tttcaaaggg gctgacagaa ctgaaacccc
    42481 gggcttgatt tcgatgcctt cgtcagtttg gtccgcgcca cgctggccga acttgctgta
    42541 gcagacaata ttgaccccga cctggtagac atccatttcc tgcaccttcg tgcctttcgg
    42601 cgcgacatac atcgtgaaac gggccatcac gtcttttgtc gggggagcct tgtggtagaa
    42661 cgccacaacg gacatgaggt tttgcccttt tcccagcttg accccgtatc cgtccggaaa
    42721 acgtgcttcc gtcatttcca aaccggcacc ggcgaagaac aacggctcgc cgtcgcatga
    42781 cacgctcggc ttgtcgttgt tcagcatgag aatgtgatgg aggtaattct tgggcagcgg
    42841 cttgccgtct ttcgtgtaga cctcggactt atagccgatc atgtaggtgt cgtccggcaa
    42901 ctggaagacc ttcttgggca tactggatgc cagatcgccg tcatgaccgg tcggcaaatc
    42961 gaccggaccg aaggtgagag tgaccgtgtg atcatcgatg gtaatcttag tgtcgcctaa
    43021 actcggcatg gttggaatgg catggtgatc gtggccaccg tcggcgtaga ccgtacttgt
    43081 gaccatcagc agcatgacca tcgatagcag cgcacgattc atagcccctc ctcgttataa
    43141 cagaccggcc gtccgtgagt gtgtgtgaga caatgtcggg aaataggccc acccgttagg
    43201 gagccgtcgg gggcggggtc agtcgcttcc acaggtaagt accgccatgt tgccggggcg
    43261 gaatattgtc tctggtgccg acacgcacgt accaccagat tcccttggct tcggtgccat
    43321 cctccgagat caccacttca aacgcgcctt cgcggtcatt gccggtctgt ttccaggtcc
    43381 cctgccagcg ccggtcgttg aagctggtgg tggcgaactg tcccccttgc caggtgtaag
    43441 ggccgttgcc gtgcgcgtcc agcgtggcga cgtatttctt attgtcttcg atttcagtga
    43501 cttcccattc gccgctcaag tccgcgccat tcggcccggg cgcctgattg tctgacacgc
    43561 gacgcactaa ggtgccgggg tcggccaggg cggtccccat tccggtgtcg ggctgtctag
    43621 tggattcgtg ccaggtcacg aggatccagg tgccgtccct tcgctccatc acccctgtct
    43681 cacgcagtgg aagcaccatg cgttgggtca ggtccgttgc gttgagataa cgaatatagt
    43741 cgagttccat cgcgaaccag gcgatgtccc ccttacgcca gaattgcagg taggtgatgg
    43801 gaagttccag tttggcgacc gaagtgaact cggtgtgcat gtcggtagcc aaagccgtcc
    43861 atcctacgta tttgtgtccg tcgatcgtgt agccgacggc gtcggcgtcg tgagacatat
    43921 acttcgacat agtgggcaga tcacgatcgg cgtttgcttg cacgatgagg cgcagagtgg
    43981 cttctgcatc gagtggctcc ttggccgctg caagggagcc cgccaggatg ctcatgaggg
    44041 aacagatgaa gaggcagtgc cgcaacgagg cacggcggga tcgacgttca atgggctgaa
    44101 agtgcatagg ggtatagggg tatagggaaa tatgtggacg aaagcctcgt gattttacga
    44161 ggcggcaatt ctgcgaggct acctggtgcc tcgagttggt gtcaatggat atttgcctga
    44221 tcgggtatct cagtaatggt gcaagccacc acggtgacgt tgtcgatccc tcccgcatcg
    44281 agcgctgtgt gaatcagtgc ctgggtggca agggaaggga tatgccgatg aggcctcacg
    44341 gtctgcagaa tgcgttcgtc ggtcaacatt tttgtgaggc cgtctgagca gagcagcagg
    44401 aggtctccat cgagaagatc gaagaagaac aggtcgcttt ccaggtgagg ggcaaggccc
    44461 atggcgcggg tgagcacatg gcggttcggg tggcatgcgg cctgcgtagg tgtgaggcgc
    44521 ccgtcacgga cgtactcttc aatcagggta tggtcccgtg tgagttgcgt gagggtgccg
    44581 gctctgacca ggtaggcccg gctatcgccg acgttcaaaa caagtaagcg tggcttgggc
    44641 agcgggagga gatgggccat cacgagtgtc gttcccatgc cgtatagcgg cgggtgctga
    44701 gccgcatggg tgaggatgga ggcatgagcc tgctccgcca tggttgtgag acggtcggtt
    44761 gcgcgctctt cctcgtgaag gtcgggggca gatgcaattc cgccgagagt cggcgcaagt
    44821 tcggcgaggg agtccatcac gagacggctg gccacatctc ctcccgcttg gcctcccatt
    44881 ccgtccgcca ctgcccagag tcgatgtcgg ttctcaacga ggaaggtgtc ttgattcgtg
    44941 ggacggacca acccgcgctc ggtctgcccg aatgccctcc atgcccatct gttctgctgt
    45001 gtgctcacgc tatccggccg gcgtcaatga cggtggaccg tagcgcaggg cgagccggtc
    45061 atatagccac tccgccagtg gttcaaggtt tctggtgtcg gcggcattat aacggacgag
    45121 ttgatccagc gctgcctcgt cgcctgcgcg gtactggtgc cagagacgaa cggcttccat
    45181 gccgtcgagt gtcagcagat cggagtcccg cgcgatgtcc aactcgcgct cgatgtgctt
    45241 gaggcctccc tgcaggccca cgcgtctcgc ggcgaaacag agatcgaagt gaggaatcga
    45301 cacccgcagg cccttgaaac aggtttggag gtaggggata tcgaacacgc tgccgaaaaa
    45361 cgtgatcagc aagtcggctt ggtcgagttc ctcctgaatc acgccaagcg tcaggtcgtc
    45421 accgctgatc agggtgcgca gtcgcccctg gcggtagagg ccgacgatgg tcacctgccc
    45481 ctcgtgcgcg gacagtccgg tcgtttcgat gtccagatag agtgcccgtg ggccgaaggt
    45541 gcggaacagc ctccagtggt cgcgggtatg caggcagccg gcaaagtacc gggcattgcg
    45601 gcggtcgagc tgctcctgcg ccgaggcaag atcgatatcg tacaggactt tgcgcgaggg
    45661 tgagatgccg ggaaggttcg gctcacggcg aaaggtcgtc caatcggcga tgccgccttc
    45721 ccagagccgc cgctccgtgg acgggccgat gccgttcaga tggacgaatg tcgaggtcaa
    45781 cataatgcgg gtcgcgatct ccctccgccg ggattgtagc cttgatttca agggataaac
    45841 aagttagagt ccgcgactgg tgcggagttt cgtgcgcttg agttgtgcag tgtatcacgg
    45901 tgtaaatgag ggaacatgac cacgaacgga tcggcgaaac gtattcgaat caaggtcggc
    45961 gggatacagt tggaggcgga gttgaaaagc acgagaacgg ctgaagaact ctaccaggcg
    46021 ctgcccgctg agggaccatt gaatgtctgg ggcgaagagt tctattttaa gattcccgga
    46081 gtgaaggacc atcgcgaaac ggcgaccacg caggtcaaag tcggtgacgt ggccttctgg
    46141 ggcgccggtc aggtgttggc gattttcttc gggcgtacgc cgatgagcat gggggccgat
    46201 cccgtgccgg ctgaccgggt caatgtggtg ggtcgcgtcg tgggagacgc ggcgactctg
    46261 cggcaggcga tgaatgccag cacgattcga gtcgaacgga ttgaacaggg ggcgtcgatc
    46321 ggataggctc atggggagtg gtcttgtaag cggccatagt ttgtcggcgg ggagtcttct
    46381 gcgatgaggc tggcaaaaga gcgggtccat catatggcgg aatcagtgat cgcacggctc
    46441 cacgagctgg gttttctgga cgtcaccggg gatcgaaagg cgcttcggga ttcgttggaa
    46501 cagacgatga cggaagagct cggagtggaa gatcgcctca atgctgaagt gcgccggatg
    46561 atgcaaccct atgaacggca gatcgagcag ggacaggtcg attaccagaa gatgttcacg
    46621 atgatcaagc agaagctggt gcgtgaacgg ggcatcattt tataggtgct gaccggtgtg
    46681 tgaatcttcg tcatagaaaa gcgcagagta gcctcactca ggactggagt ccaattcgtg
    46741 atcagcgacg ataaagccag ccatcttgcc cacctcgctc tcggcgcctt gaagaagagc
    46801 ccgcactgcc ggtttactga agatgatggg aaggtgctgc gcgagatcaa acgggtggtg
    46861 gccgcagagt tgttgttgga agcggagatc gataagaaag tacggaccaa actggcgtcc
    46921 tactcacgac aaatcgtgga agggagtccg gaatgggacg tgatgtatcg gaaaacagct
    46981 gaagaagaac aaaaaaagcg cgccaaaccc tgatccggcg tggcgagcga tttcgacgag
    47041 gttgtgcgat gagagttgct ctatggatag tgttttgcgt gatggcggtg tcgttgcccg
    47101 cctgtaccca ttccaaaccg cagccgctgg ttccgttgga attggcctat ggggccaagg
    47161 ctgacgccat cgctctgaac aatcagggga tgcaagccta tctggccggc caggtggcgg
    47221 aagccaagaa ttttttcggg caggccatca aggcgtcgcc ggattcaggc caggcccatt
    47281 acaattacgc gctcgcgctg aatgcattgg ggcaaacgga ccaggcccgc ctggagttta
    47341 ttgaggcggc gaatttagct cccggggata aggtgatctg ggattctcct gcgttacgcc
    47401 ccttcggcag tcctcaggca caaaaggggc cgcccaggga acacccctat gggacacagc
    47461 gtccggtgat cggcagcgga ccgcgctgac cggctgacac gaagcgttgt actggagagg
    47521 attgagacat ggctgacgaa ttggacgtgg gagcgaaggc gccggatttc tccttgccgg
    47581 accaggacgg cagtaccgtg acgctgaaga gcctcaaggg caagcaggtg gtcctctatt
    47641 tttatcccaa ggacgatacc tcgggctgta cgaaagaggc ctgcgatttc cgggattccc
    47701 ttgcgccgat caaaaaggcc ggggcggtgg tgattggagt cagtaaggac gggaaagcct
    47761 cgcaccagaa gtttatcgcc aagtacggct tgccctttgc gttgttgagc gacgaagagg
    47821 ccgaggtctg caaggcctac ggagtctata aagagaagag catgtacggc cggaagtacc
    47881 tgggtatcga acggagcacg tttgtgatcg atgccacggg tcggatcaag gcccttttcc
    47941 ggaaggtgaa ggtgcccggc catgtcgatg aagtgctggc ggcgctgaag gcctagcaga
    48001 atgcggaaaa agcccgccag cggtgttctc gcaagacact gccgcctcac cggctcggcg
    48061 gcgttcacaa acgtgacgcg cgttattccg cgcgtcgtga acctcagagg ctcaccgtac
    48121 ggccacggga caagagcctg tcttggcagg ctcggggtgg gcgggtacga atgaacgatt
    48181 cgccgcttcg cttgctgcgg ccttgctgga cagccatttt gcgcatcctg cgggttcttg
    48241 taattccgac acagcgcatg agtgatcgta gcgcattgcg caacaatcga gtttttccgc
    48301 cgcctgcgag ggcggcgatc caccatacgg gtcgactgct tggttgaatg acatggatcc
    48361 gctggaaaaa acctctcatg cctgccgtgc cgccgcgctc tccctcgtgc cggggctcgg
    48421 acatctctac atcggggaaa aacgcggcgg tcctctgctc ctggcagggg tgctgctgat
    48481 tgtcctctgg atggcggtgt tctcgtttgt cctcgtgccg ttgatcgggc tggtgctgtt
    48541 ttgtatggga gatacctatc tcaccgtcag ccgcgatcgc ggacttctca cctaaccaag
    48601 ccggttgtcc tctgaaaggg agcgtggacc gtctcatggc agaatggctt cgtctcgggt
    48661 cacgcacgtt cctcctgctg cttctcttcg gctggaccat ggcgggcacc ggcgaggctg
    48721 ccgagaaggt gcagggtatc ctgaccgtac atgatgcgtt gacgttgccg aatcacccgg
    48781 cccgcatcga ggcccaactc ggcgggaagg ctccgggggg aagttcccta tggggtggcg
    48841 ttggcttgca tctgtctata gacggcaagc cggtggcgat ggcgaagact aacgagagcg
    48901 gacacgcctc gttcgattac ctggcgaaga tgcggggcac gtacgctgtc acggtatcgg
    48961 tcgatgacgc ggcgtccgtt gcggcggaca acagtgacgc gacgctctgt gtgtgggagc
    49021 gccggcgtcc gatcatcctc gtcgaactgg cagccctcat ccagccggta acttccgcct
    49081 cgacgggaac ggcggctgaa tcatccctgc agcccctgtc cgatgcagcc gacgaattgt
    49141 cgcggttgac ccagtattac tacaatgtgg tctatgtgac gggcgacgaa cagccgccgt
    49201 cggagattgc cgcgatcgga aacgtgcgtc gatggttgga gtctcacaag tttccgcccg
    49261 gctttgtggc gccgccgtcg gaaggcctgg gcgccacgct ggagaccttc aagcagggcg
    49321 gctgggcgac tatgaaaacc ggcatcgtcc gcacgcggtc gtctgcagaa atgctcctgc
    49381 agcatcgatt cgaggtggtg gtggtccccg aattgcccaa aggcgagatg ccgcgaaagg
    49441 ctaaggcggc gaaagagtgg aaggacgtca gaaagaagtt gtgaggggtg acggggaccg
    49501 tgagcgcgtg cgcgaacgtc gcagggtctt tgggtttgcc cgcggcctgt gatcataggc
    49561 gagaagggat tgcgagcgtt catggctaga gggcgattca ctcccgtggt gaatcatggt
    49621 atggtcagcc tcttggtggg cgtcatctca ttgaaggagc aggagccggt atgttgttag
    49681 aaggcaaaaa ggggctgatc atcggtgtgg ccaacaagca cagcatcgcc tgggcgattg
    49741 cccaagctgc ggcgcgtgaa ggcgcgcaga tgctgttcag ttatcagaac gaacggttgc
    49801 gcgaaaacgt cgaggaattg gtgcagaccc tgcccggcgc ctcggcctgt gtgtgcgatg
    49861 tcggggatga caatcaaatc gatgcgatga tgaaacaggc tgccgagaag ctggggcgct
    49921 tggactttct ggtgcattcc ctggcctttg ctccgcgcga agaactcacc ggagaatttg
    49981 tgaacaccac ccgccaggga ttcgcgacgg ctctcgatgt gagcgcctat tccctggtgg
    50041 ctgtcacgcg ggcggcgatg ccgctcatga cggaaggcgg atcggtcgtg acgctgacct
    50101 atctcggcag tgagcgcgtg gtgccgcact acaatgtgat gggtgtggcc aaggcggcgc
    50161 ttgaagcgac ggtacggtat ctcgcccacg atctggggcc gaagaacatc cgggtcaatg
    50221 ccgtttctgc cgggccgatc aagacgctgg cggcgcgcgg cgtgtccggg atcagcaaaa
    50281 tggtcgatca ccatcgtgcg ttcgctccgc tgcggcgggc gacggagcag ggggaagtcg
    50341 gcgataccgc attgttcctg gtcagccctt tgggccgcgg catcaccgga gaggtgatct
    50401 acgtcgatgg gggttatcac attctcgggt cgttggcctc tgtcgactaa gaggcctggg
    50461 aggctgctca cgcagtccgt gtcgcgcgcc ctgcctgatc gagccgatgt catgacgcgg
    50521 catatccggg aagtgtcagg ttcgtccttc ttcctgaccc tcttcattgt tcttctcact
    50581 gttggccctg tcataccgtc cttctctgcg gaactctccg atcgggagga ggacgggttg
    50641 ctgggtgccg tccagtccgt cgaaatcaaa gaaagcttgc tggtacaaat cgatcggtat
    50701 gatccccgcg ggcgcctcat cgagcgcatt cagggcggcg gggagatatc tcaggggtta
    50761 tggccgcttc gatttgtcta tacctatgac caggcaggaa gacggactgc cgaggttgtg
    50821 caggatgcgc ggggcgccgt cgtgaaagag acgcgctcgg tctacgatga ccgcgggaat
    50881 cggtccgccg aactggcagc ctggggcgac ggcacgtttg agaacgtctc cttgtatgaa
    50941 tacgacgaag cacatcggcc gatcagaggg cttcacttca atggcgtgca agtgatcaat
    51001 cgaaatctct atgccttcga cagtgccgga cggctggtgc gtgagcggtt cgaacgaaac
    51061 tatcactaca atgcagccgg gactcaggtc gtcatgaccg gtcgattcga taccgggtat
    51121 gaagtcgcca tcgtctatga tgagcggggg caggtgcgcg aaaagatcgt gtccgatctc
    51181 cggagccgac cacagggccg ttcggagttt cgctacgacg aacagggcaa tcaaaacgaa
    51241 gaacgcatct tcaatgccac gggacaggcc accgaccgaa aagtctacca ctacgaatac
    51301 gatgtcgtcg ggaattggat caaggaagcg ctgcagtggt gggacatggc cgatggccgc
    51361 gagaggctca agcagtccca cgttcgcgag cgcagcatta cctaccacta acccccgtca
    51421 ccagtgtctg tttcaggcgc tctggtgcct ctgtgccacc gcgagtactt ggcaggttgc
    51481 tgatggggac aggatgcgcg caccattgtt ctcgtatgcg atgacgtaga aagattccgg
    51541 tgataggtcg gtgagggaaa gagcaaggaa cattgtgaac cgattgcgtc tgctgagttc
    51601 tatcgtggtt atgatgttgg cctgtgcagt cggtgaacgc ggagcctggg caggggacga
    51661 tctcgcgcca ctcctgacgc gcgggcctct gcgagtgtcc aacgacaatc cccgatactt
    51721 tgcggactcc gagaatcgaa tcgtctacct gacgggatcc cactcctggg aaaactttca
    51781 ggacatcacc gacgccttcg actatcaggc ctatctcgat cgtctggcag cattgaatca
    51841 taacttcatt cgattgtggg tcagcgaatc catccaatcc gacggagggt ggctcatgca
    51901 tcctcccgcc cggaaaaacc cggtcgtccc gcttccttat ttgcgcaccg gttctggagt
    51961 cgcattggac ggggggcctc gcctggatgt gtcccaattg aatccagcct attttgaacg
    52021 gcttagggcc agggtccagc aagccggtca gcgtggggtc tacgtctccg tcatgctctt
    52081 tcagggcttc agtgtggtcg agcgcggcgc gtggaaggcg catccattga acggccacaa
    52141 taatgtgaac ggcatcgacg gggacgcgaa cggggatgga gtgggcaaag aggtgcatcg
    52201 gtcgccgaca tctcaggtgt tgaatgtgca agaggcttac gtgcggaagg tgctggacac
    52261 actgcacgac ctggacaacg tgttatacga agtcgtcaac gaagccattc cggagtcggt
    52321 gccctggcaa tatcatttta ttcgcttcat caaacagtat gaacgggacc gtcagtatgc
    52381 agcccatccc gtcgggatga ccttcttcca actcggcgaa ttcgggggcg gcgacaacgc
    52441 cacgctgttc gacagcccgg ccgactggat ctctcccggc ggttatacca agtacgcgcg
    52501 caatcctccc gtggccgacg gccccaaggt gatgctcctc gatacggatc acattcacgg
    52561 catcggcggg gaccaggact ttgtctggga aagcttcttc cggggatata atcccatcta
    52621 catggacccg ctcggcgcgt tgcatgagtt gaccatcggg gagccggtgc tgaatgagcc
    52681 tcaacatgag cgtgcgcgaa tggcgatggg ggaagcgcgc cgatgggccg aacgcctgga
    52741 tctcaaacgg acgctgccgc acgaggaatt ggcctcgacc gggtattgtc tggcagatcc
    52801 tgggccggca tatctggtgt atgtgcctgc ctccgcgtcg tcgcattcaa cgccggatgt
    52861 cagcaggccc ctgaccgtca atcttaccgg ggcggtgggt gggttcgttg gggaatggat
    52921 cgacctggag caatcctcgg cgatgcccgt ctccgagcgg atcgaaggcg gcggagaacg
    52981 actgctcacc gtgccgtttc ggggagcagg tttgctgtat gtctatcgac agaaagacta
    53041 gcgctgtcag gaagggtgtg cggcgcgaac cgccatgcaa gccccctttt ttacggcgtc
    53101 agcggtctcg ttatgggggc gcccggcttg gcctcgtgag acaaggtcga gccttcttgc
    53161 ggcaggatgc ggaaatgcgt gacggcaaat gagacggctt gtccgaccgt aaaattgcag
    53221 cggcgaaact cttccttgtt catgcgagag cgcaagatca accggtccgg cgtctcaatc
    53281 tccagtcggt aggcgacccc gagaaaatag atgtggcagg ttcaaccggt gatagcaaca
    53341 ccctctgggg tacagtggct agggccattc agccttgttg tcaagggggc gctaatcttg
    53401 tatgcctctc tcaccggtta cgctctgtta cgtagttaaa cggcagagac ggtgtcaagc
    53461 cactcggctg tgtcgcaact ggaggagggt gcgatggggc gatgtgagcg gatgtttcgg
    53521 gctgtagggg ttgtcctcgc cctggcgttt acgctggtaa cgagccttcc actacacgcc
    53581 cagaccgtcc tcttcggccc cacacagtat acccgcaccg ccgggctgcc caatcaattc
    53641 acggccacgt ttgcgctctc cgccggcacg accgcgccct ataccatcca catcgtcaat
    53701 ggcaacgcgg atgggaccaa gcggatctcc tccgcgacga tcaaagtgaa cggcctgcag
    53761 gtggccggtc ccggcgattt cggtcaaaac gtcgcggtga tcgaccggac agtgtccttg
    53821 caaccgacga ataccctgga gattcgcctg accagcgcgc ccggtagcct gcttacgatc
    53881 agcctgttgg ataccagtgc cggatctcag ccgaccttcc tcgcgccgaa tccactgaat
    53941 cttcttgccg gggccagcgg gaccgtctcg gcggcacttg ctcctacgcc caccgccgcc
    54001 ggcactctgg ccgtgagcag tgccaacgtt gcggttgcga ccgtcccaac ctccattgca
    54061 ttcatggccg ggcaatccag cgtgccgatt tcggtcacag cggtggccgc gggctccacc
    54121 accatcactg cgatgctcaa tggcgggagt gcggctagcc aagtcaccgt gagccccgcc
    54181 gtgcccacca ttaccggctt caccccgacc agcggccagc cggggacatc cgtgacggtc
    54241 accggcgcca acttcgtcaa cgtgcagacg gtgacgttca acggcgtctc gtccacgttt
    54301 acggtgacca gcccgacgac gctgatagcg gtcgtaccac ccacggccac gaccggttcc
    54361 cttacggtgc agactcttgc cggcacggcc accagcgcgg ccagcttcac ggtgatcccg
    54421 gccccgaccc tcacgagcct gcaaccggcc acactctcaa ttgcccaggg gagcaccggc
    54481 acgctgactg tgacgctcaa tgccgttcaa cccgccgata cgatcgtcac gatggcgtcc
    54541 agcactccca cagtggccac ggtgccgggc agcatcacgg ttccggctac ccaactcagt
    54601 gccccgattg ccgtcaccgg ggtggcactc ggtcaggccg atatcacggc ctcgctgaac
    54661 ggtaccagcg cgaccagcgc cgtcaccgtg atactcgggt cgccctcgat catagggagc
    54721 aatcctgaca gtggaccggc tggcaccgtt gtgacgattg ccgggagcag tttcaacccg
    54781 acggccgcga gcaaccagat agccttcaat gggcaccctg ccattgtgag ttcagtgaat
    54841 acggcaggca cggcgcttgt cacgaccgtg ccgcaggggg caacgagcgg accagtgacg
    54901 gtcaccacac tggtgtcacc tccggccacc gggccggtct tcaccgtcac agtgccggac
    54961 tttacggtgg ccgccttgcc cacttcgttg ctcctgccgg tgactggcca aggaaccgtg
    55021 gccgtcacgg tggcaggcag caacaacttt tcaggcctcg tctccctgtc cacgctgggg
    55081 gtcccgacag gcgtctcggc ggtctttgcc tcgacgacac tcgccgcagg ccagtcaggc
    55141 atgctcaacg tgacgaccac cggggcgact acggcaggga cctatcccgt gactgtgcaa
    55201 gcaaccggcc tggtgaacgg tagtcctatc gacaagaccg tcacggtgaa tctggaggtg
    55261 ttgagcggag gcctcacgtc gtttgcaggc caagttcaag atgaagacaa ccagccggtc
    55321 aaaggcacac gagtgacgat gggggcggtt catgctatca cggacgccgg cggcaacttc
    55381 ctgctgcaga atcccccggt cggggtggac cagttattcc ttatcgatgg tgggccggct
    55441 tcgacgccag ggcgcaactt gccgattatt ccctacaagg gcaccatcgt tgctgggcag
    55501 gccaatgtgc tgagttttat tccgaagctg catgttcaga agaccacggg cctggtcgat
    55561 attgccgaca gcagcgtgca gcgtgtcgtg accgatctgg agttgcctgg ctatcagatg
    55621 accatccctg ccggcgcgac catcaccgga tgggatggac aacctaatac gcaagtgtcc
    55681 atccgtcgcg tcccgctgga ccagaatccc ctaccgccga ttccgggcga ccgcgtggcc
    55741 acagatatct acatggacta tttcggaaag gcaggcggcg gcaccgcttc ggcaccgctt
    55801 cggaaccgat tcccatcacg ttgccgaatg atttgaatct gcctcccggg acatcttcgg
    55861 aactctggtt ttatgacgag gcccctgatg ggagtcggcc taatcagtgg gccaagctgg
    55921 gaacagccac agtttcggga gacggtaacc agctcgtgcc cgatattgat ccggccacag
    55981 gaaaagccta cggtcaaccc cggttttgtt gtggtggcat ctttgccgct atgctgcgag
    56041 ccgccttcga tctcatcaac aatcaggtca acggccagcc gcaaacgccg aacggggcga
    56101 aaggggggga gccagtcgat ctcggcaccg gcgcgttcaa tttggaaaag accgacatgg
    56161 tcttgccggg gcgtctgccg atcacgatca cccgtcacta tcggagcaat ggccctgcca
    56221 ccggcccctt cggtcccggc acgagtcacg gataccaggt cgtggtgcgc gcggaagcga
    56281 atgtccgaac gctcttcatg ccggacggta gccgcatgca attccgccag cagcctgacg
    56341 gaacatttcg gaatagttca gagccgaccg tgaggggcgc ggtgtattcc gaagtgaacg
    56401 gccgggtatt gcggttcaaa gatgggacaa catggacatt cggcgccggc tcgcagggct
    56461 tcttctttct caccgccctg gccgatcgca acggcaatca gctgacgttg acgcgaagtg
    56521 gagccacgtt aaatgtgacg gccgtcaccg cgcaggatgg ccgtcaactc acgttggcct
    56581 acgatgccgg gaaccgcatc acacagatca cggatcctat cgggcgggtc gtgcaatacc
    56641 agtatggtgg gtccggtcga ttagtgagtc tcgttgatcc cgaaggtggg atcacccggt
    56701 acgtctatga tgcggcgggg cgtctggtga ccctgaccga tgcgcgagcc ctgacatttc
    56761 tgacgaacga gtatagtccg ggtagcagcc gtgttctgcg acagacccaa gtagatggtg
    56821 ggatttggaa attccggtat cagctcagtg gcgcgcgtgt cacagggcca ggctgtcccg
    56881 cgcctggtag tcaggatctc tgggtggtga tcgagggaac gcccatctgt ccaagggaag
    56941 agagtgctga aaaagtagcc cagggctacc aatttgtcgg gggtgtggtc accgcagcaa
    57001 cggtcctcga tccgctggac cacacgacca cctaccgatt caacggggcc ggacacagtg
    57061 tgagccagac cgatgcattg ggacaaacga ccatctctac ccgagacgga aacagcaatc
    57121 agattgtgag tagcattgat ccgctggggc gcaccacccg gttcgagtat gacgccgcgg
    57181 ggaacgtcat cacgatcacg gatcccaaca accaggcgac ccggttcgag tactatccga
    57241 cgtggaatcg cgtgacgaag atcacggatg cgttgaacca ggtcacggcg ctcggctacg
    57301 acctcgcgaa cgggaatctc ctcaccgtca cggaccccct caaccacgtc accaccatag
    57361 cctataattc attcggccaa ccgaccagcg tgcagggccc gatcccgtct gaaccaccca
    57421 cgacgtttgc ctacgacacg aatgggaacc tcatcacgac gaccgatccg ttggggcaca
    57481 gcacccagcg ggtctatgat gcggtgagcc gcctcacggc gcttaccgat ccccgtggtt
    57541 tgattaccca gttccgctac gatggactaa accgcgtgac agaaatcgcc gatgcccggc
    57601 aaggcctcac gcactttacc tatgatccca acggcaacct gctgaccgtc acggatgcga
    57661 agaaccaaac gacggcgtac acgtacgaca cgatggaccg gctcaagaca cgggcggatg
    57721 ggctgaaccg gaccgagacc tatgcctacg acccggccgg caatctcgcc tcgttcacgg
    57781 accgcaaagg ccaacagacc acctttcaat acgatgcatt gaaccgccgc atccaggcga
    57841 cctatcccga tgctaccacg cgatttacct acgatgccgt cgggcggctg atccatgctg
    57901 cggacacggc gcctggggcg ggcgcgctcg acttcaggta cgacagccta gacagactga
    57961 tccaagaaat caccggccaa ggtgcggtcg cctatcaata cgacgggctg ggtcgtcgaa
    58021 cgagtctggt agcgaatgga ttggtgccgg tgacgtatgg ctatgatgcc gcttcgcggt
    58081 tgactcaagt cgcgcaaggt gttcttgccg taggtcttgg ctacgacaat gcgaaccgtc
    58141 ggaccagtct gacgtatccg aatggcacga gtaccagcta cgcctacgat gtagcttcgc
    58201 gtctgacgaa tatgacgcac aatggcccga gtggcctcat cgaggggttg gcctacacct
    58261 acgattcagc cggaaaccgg accagtttgg ctcgcgccaa tgctacggcg tcgttgttgc
    58321 cgaatgccgt cgcctcggcg acctacgacg cggccaatga acaaatcgcc ttcgccggcg
    58381 cgacgctaac ctatgaccag aacggaaatc tcacgaatga cggtgtgaac atgtacatct
    58441 gggatatgcg gaatcggctg attggtctca gcggtggggc catggccagc tttcagtatg
    58501 atcccatcgg gcggcgaacg aacaagatca ttaatagcgt cgccacgcag tttttgtacg
    58561 atggtgtcga tatcatcgcc gaaattgggg gaggcgcagt cggggccaat tacttacgat
    58621 cactaattat cgatgaaccg cttattaggc agactagcga agggaatgag cagtaccata
    58681 tggatgccct ggggagcagt ttggcgttga gcagttcgca gggcaattcg gtaacggcct
    58741 atgcgtatga ggcttttgga aaaacgacgg tgattggttc gtcgtcgaat tcgtttcaat
    58801 ttaccggccg agaaaacgac ttccccggac tatcttttta tcgatctcgg tattattcgg
    58861 aaaaccgtgg tcgttttctg agttccgata gggttgggtt ggccgaaggg ctaaatgtat
    58921 atgcatatgc tgcgaataac cctgtccggt tcagtgatcc ttgggggcta tggtctgccg
    58981 aaggacacaa taagttcctc accgatcgat tcggcggagg gatggatcct gagttactag
    59041 cacaagtttt ggctgggagt gcgtctgttg atgcgcctgc catgcaaaca cccagatttg
    59101 caccaatgca tgctatgact tcggatgtat ttccaacaga gaaagtagcc agaaaagcca
    59161 tgtgcgattt tgttaataat aatatgaatg cgtttcgtaa ctatctcaac aacaatgaac
    59221 ctgccatagc ttatcatcac ctaggccagg ctttgcatcc ggttatggat acgacctcgc
    59281 ctgctcatcg tggatttcag tattggcgcc taccgcattc taaggctgaa cttgatgagc
    59341 atatagcgca agaaacgttg gacacgatta ctccagacct tgagaggcaa acactcagtc
    59401 ttatgaatgc tacgttgagc ggtgattact ctgtcttggg atgtgggaag taagcaagaa
    59461 tcgttatatg gagctgtagg caggtctgcc agaggttagc atgcatgttc gcaaaggcgg
    59521 cgccgactcc atcagtatcg acataatttt tgtgggcaca ttgtttttct gcatgattcc
    59581 cgctgatgcg caggctagtg gcatggcaat attttatatt gcttattggg tagttgggtt
    59641 ctcccaactg ctcccactgc ttttgctgtg ctcgagaaga atgcaaggcc gatgtgtgcc
    59701 ggcaagtctg gcgtatctgg gtatagtagc gacgctttgg aattatgttg tgcttgccct
    59761 tccacaagat cttgtcctca aatttagtga ggccgaatgg ttctcaagaa ccatttacag
    59821 aaaaagagaa tatctgttgg aaaagtttgg tcctgactat ggcaaaacac ttttcgacgt
    59881 attgacgttt tatgttcccc ttcttttgcc ccccatgttg gcatggggca tttatttgtt
    59941 gttaaggcga aaggtatctc atacgcctgt ccagtaactg cattcatgca gataattgct
    60001 ggcttctttg gtgcgagaaa taagctagtg cgggccagat tgccgatgat agccgtagct
    60061 gaacgagaaa cgatgagtat cgaagtatgc gtgggcgcaa ctgatagcgt gacgacgatc
    60121 acggatgcgt tgaaccaggt cacggcgttc ggctacgacc tcgcgaacgg gaatctcctt
    60181 accgtcacgg accccctcaa ccacgtcacc accatagcct ataactcatt cgggcagccc
    60241 gtttcggtgc agggaccgat atctctgaac cgcccacgac gtttgcctat gaccccaatg
    60301 gcaatctcct cacggtgacg gatgccaaga accagatcac gacctacacc tacgacaaca
    60361 tggcccgcct caagatgcgg gcggatgcgt tgaatcgggg cgagatctat ggctacgatt
    60421 tttcaaatgt ggcagctacg tttgaaccaa aaggatgtta aattgcttct tcctctgaat
    60481 cggcggatgg tcccacacct gctaattagt ctggtgttgg tggcggcatt gatagtcccg
    60541 gtctcgcttc ctgccgatac cgcccaatat ttttacgatg agctcggtcg gttggtgggc
    60601 gtggtcgacg gccaggggaa cacagccacg tacgtctacg atgaagtcgg caatctcctg
    60661 tctattcagc gcttctcgac gagcgggacg gggattgggc tctttgttgt ggcgccgggc
    60721 agcgcacgag tcggcacgac cattatgctg caggggtttg ggtttacgag cccacccgcc
    60781 agcaatcagg tccagttcaa cggcatctca gcgaccgtcc tctcctcaac tgcgaccacg
    60841 cttgtgacga cggtaccact cggtgcggca accggacagg tgaccgtgac aaatgccaac
    60901 ggttcggcga cgagcccgca gcccttcacc gtattcgtac cgccgattat ttcgggtgtg
    60961 cagccttcca cggtccccaa ggggactacc acccaactgg tcattgccgg gttcaacctg
    61021 gacggggcct ccagcgttac ttttgctcaa agtggtctga gcgcgacggt acttcccagc
    61081 agtaccgcgc agagcctggt ggtgaatttg gcggtggcgg gcacggtgcc ggacaataca
    61141 tattcctttt ctgtgaccac gccggccgga accacgcaga gcggtacggt tgtggcgcgt
    61201 gtggcgccgg cgatgacgac gttccacgtg gcgaagccgg tcagtgtgtt caagccatga
    61261 gggtggtttc atgctttggt tcgatgccat tgtacaaagg gagacgcagg ccatgaggcg
    61321 atggagtgtg tgtgtggcgg tcctgctgct gtccaattgg acgacggttg gatgggcaca
    61381 gacctttctc agtgggagca ccggggcgga cggccctttt cagcctaccg ccaacacgac
    61441 gttgccggtt cccacgagcg ggatattcca tttcaccacc gtgacgattc ccacgggggt
    61501 tactgtcacg ttcacgccca atgctacgaa tactccggtc gcaattttgg ctacgggcga
    61561 tgtatccctt gctgccggct cgattgtgaa tgtcagtggg caaccgggta ctcccggatc
    61621 gtcaacggga cctctcgtca accccggtgg gtctggagga ccagggggat ttcgcggcgg
    61681 gcagggtggg gtccgtgcac cgacgggccc cgtgacgttc ccctctgccg gccagggccc
    61741 tggcggcggc gggctcccaa cctatccgcc tccacagttc ggcggatgcg gagtcggtgg
    61801 tacctatgga acgccgtcga cttttacctc gttgttatcg ttcttcggag ggtccggtgg
    61861 tgggggacaa aatggatacc ctgggtccac gagtgtgtcc ggttcctcag gcggcggcgg
    61921 tggtggggcc attctcatcg cgtcctcgac tcgcattacg gtggccggcg ccattcaagc
    61981 caatggcggc cggggtggaa ctgcttccaa ccttacggtt ttgacggcag gttctggaag
    62041 cggtggggcc atccggcttg tggctccgga aattgccgga agcggttcgc tcgttgcgag
    62101 gtcggaggcc attgggtgcg aagccggaag cccgggcgtg attcgattgg aaaccagccg
    62161 tcgccttttt tcggggacca ccaacccggt ggcctccgtc tctaccacta tgagccccgt
    62221 tgctccaggg agtacgcctc cgttggtcaa tctaccgacc ttagcgatca gttcagtggg
    62281 cggcgcgggg gtgcctgcca acccggtcgg actatttggg gttgccgatg tcaccattcc
    62341 cctagagatc acgaatccgg tgaccgtgac agttgtcgcc accaacatcc cggttggaac
    62401 gacctttcag gtcaagctga tcccgcaggc tgcggctccc acgaccctca ccagctctgc
    62461 ctcggtcggc acgtttggat tgtctaccgc gacagccaca ttgaatcttc cgccaggaca
    62521 agtgagtgtc ttacatgttt tcgggagtgt ggctctgcct caggtcgcag gacttgttcc
    62581 ggtcatcgac ggtgagcctg ccgaacgggt tttcgtggcg ggtgggttga atgagccttc
    62641 aacgaccatg gtggtgacga agtccggcaa agaagcgccc ctgtcgcagc tttcagcaga
    62701 agaccaggtc aaggtggcaa gagcgttcga tgccatgcgc atcagcgcgc catgagtaat
    62761 ttgatcattg cgcagcatct tccagaaggc tattgctttg gattgtggct cggcgagtgc
    62821 tgctcgtgct tccgtccgta tccaagagac gcgttcgagc ttcttgtgat gcgttcactc
    62881 tcttctcgat acactatcaa ataaaatgaa accggagtct tgtgctgagc tataactgac
    62941 gagtgtgccc tcacgggctg ttctgcgtct cactgtcctc agaatatgtt taagaatatg
    63001 acgaagaaac gttcgggacg gctgccccat gcctgagtgg gtaggcagtc ggttagccgt
    63061 gctacggcgt caacggatta gtcagcggcc ggctgttttc tgttccatcc aacggagcca
    63121 tgccttcttg cggcaggatg cggaaatggg tgacggcgaa ggagacggct tgccccacgg
    63181 taaaatggca gcggcgaaat tcttccttgt tcatgcgaga gcggaggatg agtccatccg
    63241 gggtttcaat ttcgagcctg taggccacgc cgagaaagta gatgtggcgc agcttcgcct
    63301 gctgacgata tcgcgagggg tcttccgcca ccttcacgta atagggacga aatcccactt
    63361 ggagtttctg gccatcggag aaaccggctg ccggaaattc gagcgagccg acttgcacct
    63421 gcccgcgccg cacgacacct tctacgatat tcatcgaacc gataaagcgg gcgacgaatt
    63481 cagtagccgg ctcttcatag acatccgcgg gagatccggc ctgctccaac ttgcctttgg
    63541 agaacacgat gatgcgcccg gatacttcca tcgcctcttc ctgatcgtgc gtcacgaata
    63601 gactggtgac attcagatcc gtatgcaggc ggatgagcca ctcacgcaac tcctgccgta
    63661 ctttcgcgtc cacggcgccg aagggttcgt ccatcaataa caccgagggc cgcggagcca
    63721 gtgcgcgggc gatggcgacc cgctgccgct gcccgccgga tagttggtgc ggataccgac
    63781 cgcccagtcc ttcgaggctc atgagcgcga gcagctcctg aacgcgctgt tcgatgtccc
    63841 gccggttcca ttttttgatt ttgagaccga aggcgatgtt gtcgaagacg gtgaggtgct
    63901 tgaacagggc gtaatgctgg aagacgaaac cgatattgcg ttcctgcacg gtgaggtcgt
    63961 tgacgcgttt gccgtcgatg aagatgtcgc cggcggtcgg gacttccagg ccggcgatca
    64021 atcgcaatac cgtggttttt ccgctgccgc ttgggcccag aaggccgagc agctctcctt
    64081 cattgacgac gaacgagaca ttgcctactg ccgtgccaga accgaatttc tttgtgagcc
    64141 cgcgtacttc gattttcact ggcttactgc gctcccgctt ccgctacagt cgtttcgcct
    64201 cgcgccttgg cgatctccaa caccgtgagg atcgcgaacg acactgcgag tagggtcagc
    64261 gcgacggcat tggccgccac gtagttgaaa tcgacgtaac tttgatagat gtgcaaggtg
    64321 gcggtttgtg tgagcaacaa aatatttccc gagaccacga gcacggctcc gaattcgccg
    64381 atcgcgcgcg ccacggtaag ggtggcgccg tagaccaggc cccaacggag cgcgggcaag
    64441 gtcaccttca gaaacacttg ccactcgccg gctcccaagg ttcttgccgc ttcctcttct
    64501 tctgtgccga gcgtttgcag caggggcgtg agttcacgca ccatgaatgg aaaggtgacg
    64561 aacagggttg ccagaatcat ggccggcaag gcaaacagca ccttgatctg cgcctgaccg
    64621 aaaaacgtgc ccagcacggt gtccgggccg aagagcagaa tcagcatgaa gcctgcgatg
    64681 acgggcgaga ctgcaaacgg caggtcgatg acgccgctca ccagactgcg gccccaaaac
    64741 ttctgccgcg ccagcaccaa cgccgtcatg gtgccgaaga tgaggttcag caccaacacg
    64801 atcagggtga tttcagccgt gagggtaaag ccatgcatcg cttccggcct cgagatctcg
    64861 gcccagaatg ccgctagccc ctcgctgaag ctctggcttg ccatatagag gatcggtccg
    64921 accagcagga cgaggaagta gagccagacg atcccgatca acagccaacg catgcacgtg
    64981 ctcccatcac aaccaattca cgctctgccg gattcatagt ctcagattgt cgcggtcgac
    65041 accgctgact cggagccgcg ctcatccttc tgccgggtcc attgaaacaa tcttggcagc
    65101 cgttctccgg gacgacgggt caaccgttcc aagagcaaga gaaccagaaa ggagatcagg
    65161 agaatcagca ccgagacgga ggtggctgcc tgggggttgt agctctcgat ttccccatag
    65221 acatagacgg aagccacctg cgtcttcatc ggaatattgc ccgccacgat cacgatggac
    65281 ccgaattcgc cgagcgcccg cacgaaggtg aggaagacgc cggtcagcag gccgggcagg
    65341 atcgacggga ccgtgacctt ccagaaggtg atccaggggc tggctcccaa cgtgaaggct
    65401 gcttcttcct gatcgcgctc aagtcccatc aacaccggct gcagagagcg gatggtaaac
    65461 ggcatcgtca caaacaccat agccaggatg atgccgggct ggttgtagag taccgccatc
    65521 ccgccctgtt gaagccaggt gcccagcaga ctggtcggtc catagatcgc ggcgatcatg
    65581 aggccggcaa ccagggttgg aatggcaaag ggcaaatcga cgagcgcgtt gaggagccac
    65641 cggccaggga attcgtagcg caccagcaca agcgccgaca aggtgccgaa cagggcattg
    65701 atggcggtcg caatggcggc ggtttccacg gtgagccaca gcgctgccgc agcttgggga
    65761 gccgacagat cctggatgaa ccggtcccat ccggccgcga acgattgatg cgccagcgcc
    65821 gcaaggggca agaaaatcag cacgagcaca tagccgactg cggcagatcg taaggccagg
    65881 ctgagcaaga gatgagcggt catacgagcg ttctacctta ctggttacgg tcacgcatgt
    65941 tcgacaggca ccattcctat cgttggtcgt gatgattctt cacgaatcat tttctcagga
    66001 ggctgctatt tctttgtcac gtcttcgaca atcttcgtcc acagaccttg cgcgccgaac
    66061 agtttgttgc tcacctggtc ccagccgccg aggtctgcaa tcgtgaagag tcgagccggt
    66121 agtgggagcg tggtggcctg ggtaccggca ttcttgtcca acgggcgaaa gcccaattca
    66181 acgaaggcag cctgtgcctc cggtccgtgg agaaacgcga cgaacgcttc agcgagatcc
    66241 ctggccttgt ggcggtcggc ataggtgtcg acgaccgccg ccggattttc gatccagacg
    66301 gtttcggccg gcacgatgat ttcatagggc cgtccgctct tcacgcgcgg cagcaactcg
    66361 ttttcgtagg tgacgatgac atcgccgacg ccgcgttcga acgtggtcac cgattcgcgg
    66421 ccactcttgt ccatgacctt gacgttgcgt tggacgcggg tcagcaagtc cacggcctgc
    66481 gaagccgccg cctccggggc gaggggcttt ccacctgcgc gctgctccgc cagtttcaag
    66541 ccggcgccat aaatcgcgat cacatcccac atcgcgccgc cggatgtctt gggattcggg
    66601 tagagcactt ccacgccggg gcgcgcggca tcgtcccatc ccttaacgcc tttaggattg
    66661 cccttgcgta cgccgagggc cacgaccgat gcggagatca tgcccccgtg gggtgccttt
    66721 ctccaatcat gggtaatcaa tccggctttg acgatctgat cgacgtcgcc ttccagcgag
    66781 agcacggcga catcggcatc gaaacctccg atgatagccc tagcctgagc gcccgaggct
    66841 ccataagagc tgcgcacacg cacgtcctgc ccggtttttt gtttccaatg tcgttgaaag
    66901 gcggggatga tgtgtcgttc gtaggcttct ttcggcacgc tgtaggcggc caggatgaga
    66961 tcacgggttt cggcggcctg caccggggca gtggtcggaa ggagcatggt caaccaggcg
    67021 ccgagtgcaa ggccaaaaat ggttcgtgtc tgacgagtca tagcgggtcg cggctccttc
    67081 cacctatttc gaatgggcga atcgactgag cgtgtaatgg tcgagaatgt ttgcgatggc
    67141 atcccgcacc tcacccatgg cctgttggag cgggcacgaa ggtttggccg cgtagggaca
    67201 gtccgcgcac ttttggtagg cggtcttgct gacgcagctg attggggcga ggggtccatc
    67261 caggatacgg atgacctggc caagcgtgat ttcttccggc gatttgataa gggaatagcc
    67321 gccccggact cctcgacgac tggccagcag gccggcgcgt ttcaacgcga ggagaatctg
    67381 ttccaggaat tccaccggga tattctgccg ttctgcaatt tcatggcgtt ggagcacacg
    67441 cccgccatag gtctcgcaca attcgagcag tgcccggagg ccatattcgc ttttcttgga
    67501 gaacttcatg cggggagatt ttgtctagtg agttgctcaa gtttttgaag aataaaccag
    67561 cgcgcgctgt gattcaagcg tttcagatgc caggcgactg gtgtgattcg cgctccgtgc
    67621 tgccggaatc atgaatgcaa ctcattgaaa tcggaggttc tgtttcttga caccctgtaa
    67681 ccgttcttgc tagcttcagc ctgttttttg tcagccacca ccagcagact gctgctagat
    67741 ctggataccc ggtgaatttc caagacctca ttttgaccct gcatcgtttt tgggccgacc
    67801 gcgggtgtgt cgtccaccaa ccctatgacc tggaaatggg cgcgggaaca ttccatcccg
    67861 ccacgtttct ccgttccctc ggtccggaac cctggcgagc cgcctatccg caagcctgtc
    67921 gccggccgac cgacggtcga tacggcgaaa atcccaaccg tatgcagcac tactaccagt
    67981 atcaggtggt gctgaaaccg gctcccgaca atatccaaag cctctacctg gagagcctga
    68041 aacagctggg catcgacccg aagaaacacg acatccggtt tgtgcaggac gattgggagt
    68101 cccccacgct cggcgcctgg gggttgggct gggaagtccg gctcgacggc atggagatta
    68161 cccagttcac ctactttcag gaaatcggcg gcattcccct gaatcccatc accggcgaaa
    68221 ttacctatgg caccgagcgt attgcgatgt acctgcagca ggtcgataac gtgtatgacc
    68281 tggcctggac cgacggtgtg agctacggcg atgtgcatca ccgcagcgaa gtggagtttt
    68341 cccgctacaa cttcgaggag ggagaggtgc ccatgctgat ggccaccttc caggcgttcg
    68401 agggcgagtg ccagcggttg ctggacaaga agctgacgct gccggcctac gactactgca
    68461 tcaagacctc gcacatgttc aatttgctcg atgcccgcgg ggcgatcagc gtcaccgaac
    68521 ggacctccta tatcgcccgt gtgcgggcgc tggcccgtcg ttgcgccgag tcctacctgg
    68581 ccgatcgcga agcgatggga catcccttga tcaagcaatc ggctgggacc ggcaagcgcg
    68641 ccggcgctca ttcacccgtt ccaaccaaat aatccgcagg cagtacctga cgatgccgac
    68701 gaaaaagacc atgaccaaaa ctgcctcacg ctccaaggtg gccaaagccc ccaaggctcc
    68761 ggcgaccacg gaactcctgt tggaaatcgg aacggaagag ttgccctatc agttcgttgc
    68821 cccggccatg cgcgctctgc agcaatcggc cgagactctg ctgaaagacc tgcgtctcac
    68881 gtacggcacg gtccgcacga tgggcactcc gcggcggctg gtgctgttga tcgaaggact
    68941 tgcgcggcaa caagcgtctg ccgtgaaaga ggccatgggg ccttctaagg cggtggcctt
    69001 cgaccaaaat ggacagccta cgagagcggc cgtcggtttt gccgcgggcc atggtattcc
    69061 ggttgaagac ttacaagtgc ggcagacgcc caagggcgag tatctctttg cggtgaagca
    69121 ggagaaggga caggccgtgg cggcggttct gacccaggcc ttgccgcagc tccttggcaa
    69181 actgtcgttt cccaaagcca tgcattggaa ccagaccggc gtacgcttcg ccaggccggt
    69241 gcgctggctg gtggccttgt gcggcgggaa ggtactcccg atccagttcg ccacgatcaa
    69301 ggccggcaat ctgagtcacg gccatcgtgt gttgggcgcc aaggtgtcca gcccgaaggg
    69361 ctttgcggtg aagtcgattg cgcactatgt gaaggagacc gaacggcatg gggtgattgt
    69421 ggatcaggag cggcggcgcg cgatgatcct cgaccagttg gcctccctcg ccaagtcggc
    69481 tcgcgggcat ctgcaccagg acgacgatct gctggaacag gctgtctaca tggtggagtc
    69541 tccgctgacg attctgggtt ccttcaagcc gcattacctc tcgctcccga aagaaatcct
    69601 catgacctcc atgaaggagc atcaagggta tttctctctg gtcgatcaga agggcgcgct
    69661 gctgccgaat tttctcgccg tcaccaacat gaagttgtcg aacatgcagc tgatccgcga
    69721 agggaatgag agggtcctgg cggcgcggtt ggccgatgcc aagtttttct tcgatgaaga
    69781 tcgcaagacc tcgttggtcg atcgcgttgc gaaacagctg gcggtgacgt tccaccagaa
    69841 actcggcagc ctgcatcaga aaacgcaacg tgtcgtcgcg atggcggccc atgtggccgg
    69901 gcaactcggc gatgagcgac tggttcagga ttgccggcgc gcggcggaac tcagcaaggc
    69961 cgacctgttg accggcatcg tcggggagtt tccgaccctg cagggcctga tgggcgggga
    70021 atatgccaaa catgacggcg aatctccggt ggtgagcgcg gcaatccgtg agcagtacat
    70081 gcctcgcgcc atggaagggg agttgcctga gtcgctcgcg ggaaaagtgc tgtcgttggc
    70141 ggaccgcctg gacagcatcg ctggattttt cctcgtcggt atggtcccga gcggctctga
    70201 agatcccttt gccttgaggc gccatgccac ggccattgtg cgcatcgtga tcgagagcaa
    70261 gctgcgactc aatctcgccc aagccgtgcg ggcggcgcag gacgttctga gcggccagaa
    70321 tgtggtagcg gcacctcaat caggcaaagg cggggctccc gatgtcatcg gttttctgtt
    70381 cgagcgggtg cgtttctacg gaaagagcgc gcatcaggta cgggacgatg tgatggaagc
    70441 cgtgttgaag tcggccgatc gccagaccct cgacctgacg gacctcttcg acaagatgaa
    70501 ggcattgcag cagatcaccg tgcgggcgga gttcgatccg ttgatcgtcg gattcaaacg
    70561 cgcccaccgg ctgaccgaga aggaacagtg ggaccgcaag ccggtggagt ccggtctctt
    70621 ccgggaaggt gcggagtctg ccctgcatca gaccgtgcag agcagtcagc aggactatgc
    70681 ggccgcgatg cacggcgggc agtatggaca ggcgctcgat gtgctggttc gaatgaaggg
    70741 tccgatcgac gactttttca atgcggtgat ggtgaacgcc gacgatccgg cggtgcgcgg
    70801 aaaccggcta tccctgttga agagcgtcga cgatttgttc atgtcattcg ctgatttctc
    70861 ccagattatg gtacaaggga cgtagcggat ggtgaacgtt tggactctac gtcagagagc
    70921 cttcctgagt cacccgaggc gagtgccgcg gcccagctga gggcgaccaa ggtccggcgg
    70981 tttgcgtccg gcatcagcgt cgtcatgatg gtggtgtgca acgcggtgtt cctcttcggc
    71041 atctgggtga ccggcgtcaa cttcgaacgg ctggtgcgga cacctgatat ctatgactcc
    71101 agacaagaca tttgcttgcg actggcctac caacatgtcc ccggttcgga gaacccggtg
    71161 cagttctgct cggagtggct caacctcgcc gatccgaccg ggaagaccca tacgtttcaa
    71221 aaagacgcac aactcaaaca gggcgcagac ggaaagttct atttcgatta tggaccgttc
    71281 gtggactacc gactgtttgc ggtcggcgcg ttcgtggtgg ggatcatcgt gtgcggcatt
    71341 cgagtgacca ggcacgtcgt gaaccgctac cgcctgcgat tggaagccgc cgcccgccat
    71401 tcggttccga ctcattgacc tcatagcaca aggagagcgt cgcgtggcca agaaatacgt
    71461 ctattatttc ggagatggga aagccgaagg caccggcaac atgaaggaac tgctcggcgg
    71521 caaaggcgcc ggactggccg agatgacgaa cctcaaagtg tccgtgcccc ccggctttac
    71581 gatctccacc gaagcctgcg tcgaatatta caaacgcggc aaggcctatc ctcccggcat
    71641 gatggacgaa gcgctgcacg ctctcaagcg gatcgaacgg tcgatgaagg ccggtttcgg
    71701 cgatccggac aatcccttgt tggtgtccgt gcgctccggc gcccgcgcgt ccatgcccgg
    71761 catgatggat accgtgttga acgtcggctt gacgaccaag accgtccacg gcctggccct
    71821 caagacgaag aacgagcggt ttgcgcagga tagctaccgc cgcttcatcg gtatgttcgg
    71881 cagcatcgtg atgggcgtca atcgcgaaca tttcgaagac attctcaagc ataagaagcg
    71941 ggatctcggg gtgacgcagg acacgcacct cgatgccaag gcgctcaagg aactcgtcgt
    72001 cagcttcaag gagttggtga aggaagagac caagcgggat ttccccgatg atcccctgga
    72061 gcaactgcgg atggcgatca acgccgtgtt ttcctcctgg tacggtgcgc gcgccgtgac
    72121 ctatcgccgt ctgtacaaca ttcccgaaac ctggggcact gccgtcaacg tggtcgccat
    72181 ggtgttcggc aacatgggcg aaaccagcgg caccggcgtg gccttcacgc gcgatcccgc
    72241 caccggtcag cgtcagttct tcggcgaatg tctgacgaac gcgcagggtg aagacgtcgt
    72301 tgccggtatc cggaccccgc tgcccgtcaa ggaactcgaa aagttcatgc cggaggctta
    72361 caaggatctc gaagcgacct acaaaaaact cgaacgccat taccgcgaca tgctcgacct
    72421 ggagttcacg atccaggaag ggaagctcta catgttgcaa acccgcgtcg ggaagcgcac
    72481 gggtgtggcg gccgtccgga ttgcggtgga tatggtgaag gaaggcctca ttaccaaaaa
    72541 ggaggcgctc cagcgcatcg ggcccgatca actggcgcag tatctctatc ccattttcga
    72601 cgcgcaggaa gagtcgcgct gcacgccgct cggcaaggga ttgccggccg gtcccggcgc
    72661 cgccgccgga aaactcgcat tgacggcgga ccgtgcggtg gagatgaaag ccgcgggcaa
    72721 tcgcgtggtc cttgtccggc aggaaaccag cccggacgat attcacggca tgaacgccgc
    72781 gttgggcttt ctgactgcgc gtggcggcat gacgtcccac gcggccgtgg tcgcccggca
    72841 gatggggaaa gtctgcgtgg ccggctgtga agcgatcgag gtgctggata gtcagtcggt
    72901 gcggatcggg acccaggtgt ttcgcgaagg cgaatacctg tcggtcaacg gttcgaccgg
    72961 caacgtgtat ggcggcgaca ttcccgtcgt ggaatcagaa gtgattcaag tgctgcaggg
    73021 caagatggaa gcctcggctt ccgagaagta ccaattgttc gagtcggtgc tgaagtgggc
    73081 cgacggagtg cgcaagctga aggtccgggc gaatgcggat gtgccggatc aagcccgtat
    73141 tgcgcgcagt ttcggcgccg aaggtattgg cctgtgccgc acggaacata tgttcttcgc
    73201 cgaagaccgt atccagatca tgcagaagat gattctggcg cggaaacgcg aagagcggga
    73261 aatgtatctg gatcagctgc tgccgttgca gaaacaggat ttcatcggtc tttatcgcga
    73321 gatgaagggg ttcccggtca cgatccgtct gctcgatccg cccctgcacg agttcctgcc
    73381 gaagcgcgaa gatctcatgg tcgaaattgc tcagctcgaa ctcaccagcg gcgcgccgac
    73441 ggtgttggag gaaaagaagc ggttgctggc tcgcgtggaa gagctgcatg agtttaaccc
    73501 catgctgggt ctgcgcggct gccgtctcgg gattacgatg ccggaaatta ccaagatgca
    73561 ggcgcgcgcg atcatcgaag cggcctgtga gctggcgaag gaaggcacga agatcgtgcc
    73621 tgagatcatg attcccttgg tcggcatggt ctcggaaatg aaggcgcaga aagatctggt
    73681 ccgcgaagtg gccacggaca cgatgaagcg gtacggcgtg aaattgtcgt acctggtcgg
    73741 caccatgatc gaattgccgc gcgccgccgt gaccgccgat cgtatcgcgg aggaggccga
    73801 gttcttctcc ttcgggacga acgatctgac ccagacgacg ttcgggttct cccgcgacga
    73861 cgccgcgaag ttcatcgatt tctataagac ggccaatatt ctcgacagcg atccgtttgc
    73921 ggtgctggac cgggaaggcg tcggatcgct catgcgcacg gccattgtgg gcgggcgcaa
    73981 gacccgtccc acgatcaagc tgggcatctg cggcgagcat ggcggcgatc ccagttctgt
    74041 ggaattctgt catcaactgg gcctggacta tgtgagttgt tcaccctacc gggtcggcat
    74101 tgcgcgattg gctgcggcac aggcggcgct ggcggaggct gaaacggaaa aagccaagcc
    74161 gaagaaagtt caaccgagca ccaagcgggt ggcgaaatcc aagccggcca agaagtccgc
    74221 aacgcacgcg ccggtgaaga agcggcccgc cgccaagcgt gtggtccgtc gccccaagcg
    74281 tacgaaacgg tgaggttgtc ttcccgcgat cgtgaataca tgaccctggc gcttcgcctc
    74341 gcggcgaagg gccggggatc caccagcccc aatcccatgg tcggcgcatt ggtggtgacc
    74401 cgcggccgga tcgtcggtca gggttcccat cgcaaagccg gcggtccaca tgccgaagtc
    74461 atcgccctca gccaggccgg ctcacgggct aaaggcggca ccctctacgt caccctcgaa
    74521 ccctgcagtc atctcaaaaa acgcactcca ccctgtgttc cgctggtcat tgcctcggga
    74581 gtccgccgcg tcgtggtggc catggtcgat gccaacccgc aggtgaaggg caatggggtt
    74641 gcgcagctga cgcgggcggg aattcgtgtc gatgtcggtt gctgcgaggc cgaggcgagg
    74701 cagctcaatg aagcttatct tcattgggtg cagaccggcc gtccgtttac gatcctgaaa
    74761 gccgggatga cgctggacgg gcagatcgcc actgcgaagg gcgagtcgca atggattacg
    74821 gatgaggccg ccagacagca ggcccatcgc ttgcgggctg atgtagacgc gatcctggtc
    74881 ggcatcggca cggtgttacg cgacaatcca cagttgaccg cccggctgac cgacggcccg
    74941 gcgcggttgg ctccgcggca gccgttgcgc attgtggtcg atagtcgatt gcgtattccc
    75001 ctcaaggcca aggtgctgca ggaccagcag acggctcaca cgttgatcgc tacgacagcg
    75061 gcagcttcag ggcgtaagat tgcgcttctg aggaagaggg ggatcgatgt gttggtctta
    75121 ccgaaggctg gtggccatgt gaacttgccg gtgttgtggg cgcgcctcgg tcagcttggc
    75181 gtcacaacgg tcttggtcga aggcggcagc gacctgaacg cggccgtgct gcaagccgga
    75241 ttgccccagc gattgatgtg gtatgtcgcc ccgttgctgc tcggcgggca ggatgcgaaa
    75301 ggaatcttcg gcgggcagtc gccgcaccga ctccggaatg ccgtacccct gaagaatctg
    75361 cgcatcgagc cggtggggcg tgatatgttg atacaggctg acttctcgac tcgttaggag
    75421 cgttgtgcga cattctttcc ggcccttcga ccgatttcac caacgcagac ttttcctgct
    75481 cccggttctc ttctcgtgcc tgctatgggc gagtctttcg attccggcca cccacgccgc
    75541 ggaggagggg gcctcgaccg acctggcctc gcaggtctgg caatatctca cgacccagga
    75601 tgccgaacag cagcgcaccg tgctcgcgtc gattgtgcag cgaccggacg cgacggtgca
    75661 ggccgtacag gaactgttgc gcaaaggtcc gcctcacggg ttgcagcccg tgggactcct
    75721 gccggatgag cagatcgtcg tccgcgaacg accgtatcag ctgagcttgt ccgtccctca
    75781 gagttatgaa ccgacccgag attatgccct gatcgtctgt ctccatggcg ccggattcac
    75841 cggcgaggcc tacttagatc gatggaaatc acgcttgggc gagggctaca tcctggcctg
    75901 tccgacctat ccagccggag cctggttcac cagacgcgcg gaagatctgg tgctggcgac
    75961 cgtgcaggcc gcgcagcagc ggtatcggat cgatcccaac cgcatcttcc tgagcggcat
    76021 gtccaacggc ggtatcggtg cctggctgat cggcatgcat catgcgcccc tgtttgccgg
    76081 acttgcgccg atggccagcg ggatcgatga cgtcttgttt ccgtttctgg agaaccttcg
    76141 tacgacgccg acgtacatca ttcatggatc gcaggatcag gtgatgccgg tggaattgag
    76201 ccggaagctg gcgggagaac tcaagaacct cgggtatccc tacatctatc gcgagcatga
    76261 tcgcacccat gcgatggccg ggggccactt ttttccgcgt gaagaacttc ccgatctggt
    76321 gaagtggttc gacgaccagc ggcggactcc tgttccgaag gcgctgacag tcgtgcggga
    76381 tgcgagccat ttgctgccgt tcggctgggt gcggatcgat gcgacggacc agatcgcatc
    76441 cttttcagaa gatttggtcg ataagcggga tgtgagtatc cggcagcgca tctacgccag
    76501 gctgaccgct caggtgaccg ggccgaatcg gattgaggtc aagaccgatc gtgtccggca
    76561 atacacattg ttcctgaacg aagaccttgt cgatctgtca aagccggtcg tcatcaccac
    76621 cgatgggcag gtttccttcg agggcgtcgt cacgccacag gtcgatacgc tgctgcggca
    76681 agcgcggctc aggcaggacc ccgggcaact gtatcccgct catctcacgc tctcactgcc
    76741 gcaacggcct tcatgagcag gttcaggccg gtcgcgcgac gagccggcct gatcggggtc
    76801 gtgctcacag ccgggctggc ggtcctcgcg tctgtcgggc gaactgaatc ggaccggtgc
    76861 tcccttcccg cacaccatgc cggtgtcgtc tttccgctgg atcaagtcga ggccgcctgg
    76921 gcctgccgac ttgagccgat cgtcacgcat tacaccaccg ccaataaagt cggaccacaa
    76981 cgcacgcctt tgccgcaacc ggtcttcctc tatctcctgg atcatccggt gatggccgcc
    77041 atgctggtca accgcctcga tctcgggttg tataaggccg agcagcgcgg gcaggaggaa
    77101 ttctgggcca ccgacgggga aggcacggaa gggatcgtcc attccctgtt tcgagatcct
    77161 acgacgcgta tctattacgt ggagggaagc catgacggac ggtttctgcc gcgggtgagc
    77221 ggcaaggcgg tggtcctgtt gaggctgcat cctgtgaccg acaaccgggg agtcgaatcg
    77281 atcgacagta cgatggtcgc ctacctgcga ctcgacaatc gcttcctgtc ggggatgctg
    77341 tcgctgctcc gtccgctgat cgggaatgtg gtgaatcgtc agatcctgaa agccttcgat
    77401 gccgcccgcc ggttaggtgg cgcgatgcgc gaacattccg agcaggtcct gtttgaagcg
    77461 acggatccac cggctctgcc ggatgacgag gtggccttct tgaaagccgc ccttgcgggt
    77521 ctgcacaatc ctgtcgaatc gcttcgccca actccctgat gagatatcca tgacgacttc
    77581 ccgaagtttt ttttatctct gcacccttgg cgtgttctgc ttcatcagct acaacctcgt
    77641 ccgcatgccg gtcttatccc tctttgcaga atcgctgggt gcaggccctg agcggatcgg
    77701 gttgatcgtg tcggtctcga ccatcaccgg tgtgttgctg aaattgccct cgggtgccct
    77761 ctccgatatc tatggacgga agatgctgct gcgtatcggc gtggtggcgt tcggactgcc
    77821 gccgttcatc taccccttca tctcggatct caacgcgctg acggcgctgc ggtttgtgca
    77881 tggcctggcc acggccatct ttgcgccgag tgccctggcc accgtggccg atctgtataa
    77941 ggaacggcgc ggcgcagcgc tgggaaccta tacggcctgc acccaatccg gttctctgct
    78001 cggcccgttc ctgggtggct ggttggccta cacggcagga ttttccacgg ccttcgtgac
    78061 ggccggagtg ttcggctgta tcgcgatcct gatctttttc agtctccatc tggatgaacc
    78121 gccgccgcgc gtgcgcgagc ggggtttggc accggtcatg gctgagatgg ggaaagggtt
    78181 tctcgccgtg gcgcgcaacc ggaaggtgtt gatcaccagt tcaacagatg ccgccaagat
    78241 ggtggccaac ggcgccctga tggcattcct gccgctgtat ggtctttcag tcggcctcaa
    78301 cgcgggacag gtggggctgc tgtttagtgt gcaggcggtg acgtcatttc tttccaagcc
    78361 ggtgatgggg cgggtctcgg accgcgtagg gcggcagccg ctgattctgg ccggcctcct
    78421 gatttgcgcc gcgaccttca tcagcatgcc ccatgtcggc tcatttgctc tgttgctggt
    78481 gctttcgtcc gggtttggat tcggagaggc tgtggtgtcg tcgtcgtccg ccgcgctggt
    78541 cgcagacagt tccgaattca agcgattggg ggcggggatg ggtatgcagg ggacggtgat
    78601 ggacatcggc catgccagcg ggcccctgct ggccggtctg ctgatcgccc atgtgagtta
    78661 ccagggggct tttgccgtga ttgccggtct ccagatcctg gcggccatcg ccttctgggc
    78721 cacgatgaga actctctcgc ggtagtgcta taatgccgcc gcttttcgcc tggggacggt
    78781 cacattttgc aaaaggaagc ggagaaggat ggacacggat tttatcgaaa cgctgcagcg
    78841 cggcattgcc gtcgtgggcg ggatcggact actgggtttt tgtctctatc tcttgaagac
    78901 tcgcaaggga gcgtaagcca tgttcagcgg catcgtggaa gagatgggcg cggtctccgt
    78961 actgaataag gggctggccg gcacgcgtct gaccatcatc gcctcgacga tcatgagcga
    79021 tctcaccatc ggcgccagcg tgagcgtcaa tgggacctgc ctgacggcgg tcgccagaac
    79081 cgaccatgac ttctcggtcg atgtctcgcc tgaaaccctc gcggtgacga cgctcggctg
    79141 tctcacttcc ggttcgcccg tgaatctgga gcgtgccatg aagttgaacg agcgcatcgg
    79201 cgggcacatg gtgtcaggac atgtggatgg catcggtgcc atccggagcc ggcatcagga
    79261 cgggaacgcg ctgattctgg agattgaggc gccgaaggag attctgcgcc tctgtgttca
    79321 gaagggatcg atcacggtcg atggcatcag tctcaccatc aacgacgtga ccgaacgctc
    79381 gtttgtcgtc tcgatcattc cccacactgc aaaagtcacc acactcggac tgaaacaggt
    79441 gggcgacaag cttaacctgg aatcagacct gatcggcaaa tacgtagagc gcctgctcca
    79501 ggagcgcggt atcctccctc caaaaccaac gccggttatt gatacggatt atctgaaacg
    79561 gcggggattg atctagagca atggaggtca cctgatcgac cttcttggcg cgctcaatgc
    79621 gcgccaagaa ggtcgatcag gccgtagccg cgccctctca cttcaccaga aatgccttgg
    79681 aatcttcacc ggacgcagtc gtgacggtca tcatagccat gcctgtgcgt ctgccggtcg
    79741 gtacggttgc cgtgatctgg gtgtcgttga cgaatgtaaa tgcggcggga tacccgggac
    79801 cgaaggatag tgagcgcaga cagtcggcgg gaccgaatcc tctgcccgag atcgtgacct
    79861 tctcgcctgc tttggcttca tccggcgtga ccgactggat cttcggtgct tctccgaagc
    79921 aggcttgcgc cttggctgcc gtttctgccg aagtgtactt gggggcctgg tctttggcgg
    79981 tggccgccgg cttcgccttc tgacttccgc tcttcgcggt cgcctttttc tcgggagtct
    80041 tctttgctgc tggtgcggcc tgttcagccg gcttcttggt gtcctcggcg gccgcgacgg
    80101 ggatgacccc tcccagcagc acaaatccca tcaccgtcga ccacaccaca ctctgcttct
    80161 cgctctgcct ctgcatgacc ggcctccatg cacacaataa aagatgatgc attgactgaa
    80221 gacgaatcgt ctcagtcttc gatcgtcgaa atatcgccca cgtcctgtcc cagttccttg
    80281 gctttgagga cccgccgcat gatcttgccg gacctggtct tcggcaacga cggcacgatg
    80341 tcgatttctt gcggcaccgc gattttcccc aactccttca gcacatggtc tttgagcgat
    80401 tgcaccaact ctttcgagtc ctggtagcct tgcttcagga tgacgaaggc cttgatcgct
    80461 tcaccggcgg tgcgatgcgg cttgccgatg accgccgctt cggccacggc ttcgtgactg
    80521 accagggcgc tttcgacttc cgccgtgccg atgcggttgc cggcgacttt gatcacatcg
    80581 tctgcgcgac ccatgaacca catgtacccg tcgttgtcct tccggcagac atcgcccgcc
    80641 gtataacagt tggggatcgt gttccagtag accttgtagc gatcgggatc tttgtagatc
    80701 gtgcgcatca tggagggcca gggcttcttg atcacggcaa agccgccggc attgttcggg
    80761 aggctgttcc cttgcttgtc caccacgtcg gcctcgatgc cgagaaacgg tctggtggcc
    80821 gatcctggct tcagcggaac ggacggtagg ggcgtgacca ggatggagcc ggtttcggtc
    80881 tgccaccagg tatccatgat cggcttgtcg ctgccggtca cgcggtaaaa ccattcccag
    80941 gcttccgggt taatgggttc gccgacgctg ccgaggatgc gcaacgttga gaggtcgtac
    81001 ttcttgggcc agtcctcccc gtacttcatc aggagccgga ccgccgtcgg cgtggtgtag
    81061 aaaatcgaga cgccgtagcg ggcgatgaga tcccaccagc ggccggggtt cggatagtcg
    81121 ggtttgccct cggccatgag gatcgtcgcg ccgttcagca gcgggccata gacgatgtaa
    81181 ctgtggccgg tgacccagcc cgggtcggcc acgcagaagt agacgtcttc ttccttgagg
    81241 tcgaacacat atttggtcgt gatgtaggta ccgaccatat aaccgccatg cacatggacg
    81301 acgcctttgg gtttaccggt cgtgcctgag gtgtagagaa tgtagagcgg agtttccgca
    81361 tcgagcgctt ctgcatcgca ggtggcgctc tgtgcttgca gccactcgac ccaatcgatt
    81421 tcttttggag cttcgagtgc ggcacctgga gattcgcgtc ggacgaccac caccttttcg
    81481 acggacgggc tggtgcgcac ggcatcgtcg acgacgccct tgaggttgat ggtttttccg
    81541 cgatcgtacc cgacatcagc cgtgatgacg acgcgcgctt cggcgtcctg gatgcggctt
    81601 gcgagggccg gggcgctgaa gccggaatag accacgctgt ggatcacgcc gatacgggcg
    81661 caggccagca tggcgacgac ctgttcagga attttcggga gatagatggt gacgcgatcg
    81721 cccttttgca ggccaagcgc cttgagggca ttggcgcacc ggttgacctg acgcaacagt
    81781 tctccatagg tgaagatgcg ttcctcaccg ttctcgccga cccagatgat ggcgaccttg
    81841 ttacgccgcc aggtgttcgc gtggcgatcc aggcagttat aggcgatgtt gcaggtcgcg
    81901 ccgacgaacc atttcgccca ggggtagttc cagtcgagca ccttgctcca cggcgaaaac
    81961 caatccagct ccttggccac accgccccag aacgcttccg gatcggcgat ggaagcctgg
    82021 taagcctgct catagtcctg aatatgagca gccgccttgg tcttggcggt cggctgaata
    82081 acacgtcctt ctttcagtag ggtatcgatc ttctcgtcca tcagtgccat gcctccacaa
    82141 agcgcgggcc tgtccggcgc gcgattcaat catcaccggc attatgcgta tggggcgaag
    82201 gaactgcaac cctcagcgcc cctgtgagac cttgcctgat gcagacggag gccgcgtggc
    82261 gcaaggccgg agcactccgc ctttcttgac agtcaggagg agacgagggt acgctgattg
    82321 cgttgcactt cactggatta accccttcat gcgctcgtct acattccccc atcttcgtcg
    82381 gatgttcgta tgcctggtgg ccatgtgcgc tgctggaacg gttggaattc tctccattgc
    82441 ccacgcgcag gttggaaagc cggaaggact ctattacaag tcctgggcgg tcgtggtcgg
    82501 tgtcgagaac tatctcgtgg caccgaaagt gcccggcgcg ttggagagtg cgcatgcggt
    82561 ggcggcggcg ttgcgggggc tcgggttcga tgaagtgatc gagctgcagg ataaagaggc
    82621 aagttccaaa catctgctcc agattctcaa tgactatttg ccccgcaaag tagggcgtca
    82681 ggatcgcgtg ctgttctttt ttgccgggca tgcaggggtc actcaggatt ctcaatccaa
    82741 agaactgggc tatctggttc cctgggatgc gcagctgaac aatcccgcta aagccatcac
    82801 gttcgatcaa ctcaaagaat tcagccgacg gtccgcttcc aaacacatct tgatgttgtt
    82861 cgacgcgaac atccgggggt gggaggtgac cgcgccgcag ccgctttcac tcgaaggccg
    82921 tttgtcgccc gaagatgaca cggaaaaacg cgccgtccag gtcctcacgg cggctgagaa
    82981 agaggaggcg gtcggacgtg ctccgggcca gagcgccttt gtaacggcat tggtcgctgg
    83041 gctgaacggg gctgccgatc tcaacaagaa cggctgggtg atggccagcg aactcgcggc
    83101 gcaggtgaag caggacgtgg aggccgcgac gaagggggcg cagcacccgc agttcgtgca
    83161 gttggagggc gacggggacg tcattctgat tgaaggacga aaaggcgcct tccaggccgg
    83221 gaaggaaccg acgagtgagg cggaccggat gagggaggcc cgcattcagt atgaacaggc
    83281 ctttgcattg ctgcagcagc aaaagtcggc cgaagaggcg ttggaacggc tcaaccgcgc
    83341 gatcggctac gatccgtcct tcggcgatgc ctacgtatta aagagttaca tccgtttgga
    83401 agtgctgccg aatctggagg agtcgctgct cgccgcccgc gcggcggtgc aacatgcgcc
    83461 gcagaatccc gactcccact actccctggc actggccctc acaaagaagg gacagtacca
    83521 ggaagccaag caggccatgc agcaggcatt agtagtgaac ccggcctacg gggatgtcta
    83581 tttctcgctc ggcgagctct acgccgacca tttgaacgaa ccgcaaaagt ccgtcgacgc
    83641 gttccgtcga tatctggagc tggggggaca aagtgaacgc gcgatccgcg ccgttcaagg
    83701 cagtgctcct ccagccgata aacctgctcc ctagcaggct gcgtagaaac tcggcttgtg
    83761 cgcgatagct cgcgatcagc tcacgtgccg tgtcgatgtc agaatcgctc gcaggctgcg
    83821 taaaaaggct gtccagcaag gccggcagcg agcgaagagg cgaatcgtac tcgggccgta
    83881 cggtgagcct ctgaggttca cgacgcgcgg aataacgcgt gtcacgtttg tgaacgccgc
    83941 cgagacggtg aggcggcagt gtcttgtgag aacgctgccg gcggactttt tccgcagcct
    84001 gctagtcttt tcctccgcct ttcagatacc ccaatccaat cttcaacgcc cggcgagtga
    84061 tttccggcca gcggacctgg ggatattccg ccaacacggc ggcggccttg ggatcctgga
    84121 tatcgagttg aaccacttta ataatgccgt cttcaatctg cacatcagcc atgtgtctgt
    84181 ctcctccgta taaacttgtg tctaggagtc tgtccgagta atgccgttct gctgcgacga
    84241 atgatctgcc ggcatctgcc tcgttctcgg cccgtaaaaa tcctcaacgt attcagcgaa
    84301 tacgcttccg gttttttcga gcctgcggcc tcgcatccgc cggcaactcc ttcgtctcgc
    84361 tacgaaggat tactcggaca ggctcctagg gtagatgaat cggccctgtg gtcaaccgga
    84421 acagtcgttg cgcgttgaca gtgttagggg tgcatgttag cataaaatct gcttttttcg
    84481 cccaactgtg tcgggtgaca gtgagccaca cacgtctctc tcccatcatc ttgccacaca
    84541 ccacgaagcc gtcagcgttc agctgtgtca caccaaggag gaatcgtatg cgcagagtag
    84601 agggggcttt ttccaagttc accggcagcg cgttggcgct cgtactcctc atcgggctga
    84661 ctgcctgcgg cggccctccg aactgggtca agaagggatc cggcgccttc aacgaaaaga
    84721 gcgataagtc tttttatggt gtcgggtcgg tggtgggcgt gcggaacgag cccttagcct
    84781 gggacacggc tgagaaccgc agccgcgcgg aaatcgcgaa aaccttcgaa acctacacgg
    84841 cctatctcat gcgcgactat gcggcctcaa cgaccgccgg cgacttttcc cgcaacagtg
    84901 aagaacagaa catcgaacgg gcagtgaaga ccttctcagc agtcactctg aacggtgtga
    84961 agccgatgga tcggtacaaa gatgagaagt ccggcaccta ctacgtcctc accaagctga
    85021 gcctcgaaga catgaagaac aatctggagc aggcgaagga gttgaacggc caggtccgcg
    85081 actttgtgcg aaagaatgcc gaccggttgt tcgatcgtct ggagaaggaa gaagacaagc
    85141 gggcgacgaa gtaatcggac gtgccggtgc gcgctgacca ggaggagaga gcatgatcga
    85201 gtggcgggat cgttcagtgg gtcggacctt cgcgacggca gcgctggtgg ctgtggccgc
    85261 ggcggcgagc ggatgtgggc acgaaacaaa agtgacgcgc gtcgataccg gcatcgtcac
    85321 ggacctcagc ggacgctgga acgacaccga ttcacaaatg gtggctgaag ccatggtgcg
    85381 agaagccctg ggcaatccct ggctcggcaa ttttaccaag ggcaagagtc gccagccggt
    85441 cgtcatcgtc ggaaccgtcc tcaacaagag ccatgaacac atcaacgtgc agaccttcat
    85501 cagtgatctg gagcgggaac tgacgaactc gcaaaaagtg acgttcgtcg ccggcaaggg
    85561 cgagcgggaa gaggtgcgcg aggagcggcg cgagcaggcc gtgcacgccc gtgaagacac
    85621 gcagaaagct cccgggaaag aaatcggcgc ggactacatg atgcgcggga gcatctccac
    85681 gattctggat gaacaggatg gggccaaagc ggtcttctat cagatcgacc tggaactggt
    85741 ggatatggag aacaacgtga aggcctggtt cgggcagaag aaaatcaaga aggtcgtcga
    85801 gaagaaacga acgatttttt agcccgacgt attctgttga gacgccactc cccatcgacc
    85861 acggcctcct ttcagggagg ccgtttcatt tctctcccct ggatgcgcgt tgtcttcctg
    85921 ggcctgttgt ggaccctgtc cgggtgcggg ttctccgcca atcattatct cctcatcgac
    85981 cagagtttgc tggcgaacga ccccaggcgg gcagacgcca tcatggcgca ggccgaatcc
    86041 gaatacggct cgcgtaatcg tctgctgtac gacatggatc gcgggatgac gctgcatctg
    86101 gccggggact atgtgcagag taacagcctg ctggaacagg cctcgctaga agtggaacgg
    86161 ctgtataccc ggaccatccg cactgaaacc gccgcgttcc tcaccaacga caccatgctg
    86221 ccctacgaag gtgacccgta cgagcacgtg atgatcaacg tcatcaaggc gctcaactat
    86281 gcggcgatgg ggcagttgat ggaggccgtc gttgaggcgc ggcagatcga tcatcgattg
    86341 aacgtgctgt cggacagcgc caaagaaaaa gacgggtatc gggaggatgc gtttgcccgc
    86401 tacctgaccg gtgtgttgta cgaggcgacg ggcgatctca acaatgcctt cattgcctac
    86461 cgtaaagcct atgagattta tgagtccacg cgggggtggg ccaggacgcc catgccgccg
    86521 atgcttcgcg cagatctgct ccgaaccacc gatgccctgc acatgacggc tgagttcgag
    86581 gagtatcgtc ggcagtttcc cgacacagga tgggtgtcgc tgcaggggca acccgatctc
    86641 gctcaggtgg tggtgatcgg ctataatggc cgcgcgccgc gcaaggaaga ggtccatctc
    86701 gatattccgg tcagcctggg cgcactgcaa ctggtcctgc tgaatcgagg cgtgattcac
    86761 gcgtccaacc gtcaggagcg tcgagtggcg gacagtgtgt tgtatggcct gaacgggcgt
    86821 gtcgtgcggg tggccctgcc ccggttggtg ccacaaaaaa cgcaggtctc gcacgaatcc
    86881 atcacgctgg tgccgcgaaa cggagagccg atcaccggac aatcgcagct gatgtataac
    86941 agtacggcgt tggcggaaaa atccctgtcg gaccgcatgc ccggaatcac tacgaaagcc
    87001 cttgcgcgcg cggccgtgaa attcaccgca gccgaagcgg cgacccgtgg atcccaacat
    87061 gccgtgaaca agggggatgc cgcgtgggtg gggctcttgg tggggttgct cacgcatggt
    87121 ctggcggtgg cgtccgaagt cgccgatacg cgaagttggc ggaccctgcc ggacgaaatt
    87181 cagatttccc gcctgtgggt cccggccggc gaatacgaaa gtcggattca accggtgctt
    87241 cgaagcggcg gggctcccca ggccggtgtg tttcgtccgc tggtccttcg cgccgggcac
    87301 accgtcttcc tgatcgagcg ggtggtgtta tgaagctggt cacgggtcga gggcgagcgg
    87361 ccgttctctc ggcattgctg ctgttgcttg ccggctgcgg atggttcggc ggaactgccc
    87421 gtcctgcgtg gatcgacgga ggaagccccc agtttccgtc agcccagtat ctcgtcgggg
    87481 tgggtcaggc cgattcgcga ccgcaggcaa cggagcaggc ctatgctgcc gtctccagaa
    87541 tttttaaggc cgagatcacg gcgcaggcga aagactggga atcgtacctg gttgtggagt
    87601 cccgcgggca gacgagcacg gagcgtcgcc tcaccatgga caacgtgacc cgtgtgacga
    87661 ccgacaaggt gctggaaaat gtgcaggttc tggatacctg gtttgaccaa aagacgcgac
    87721 agtactatgc tctggcaggc atgaaccggg cgcaagccga agcggcgatg gtggaacgcc
    87781 tcaatgaact cgatcgcacg attcagaccg aagtgaccga agcgcatcag acccaggaca
    87841 agctctcgcg cgtgcgtaat ctcaaacggg ccgccaagaa cctggtgctg cgcgaggcct
    87901 ataacaccga tcttcgcgtc ctgcgctcga acggtcaggg caatgcgtcg acgtatcgtg
    87961 tggcggagtt gactgctgaa ttggaacagt ttcttgccaa gaatctcgtc atggccgtcg
    88021 atctgtctgg cgatcaagcc gagccgcttg agcgtgcgct cgtagacggc ctcgcacagg
    88081 aaggcttcac ggtggtgggg cgcggcgccg gtgccgttcc tgcagaatta ctcattagcg
    88141 ggtcggtgcg tctctggccg atcgaagtga acgatcccca cttccgctat gtccgctggt
    88201 gcgcggaatc cgtgatcgaa gaggtcgcga cccatcgtgt ggtcggggcc ctgtcgaaag
    88261 gcgggaagga agggcatgtc acggagcgcg aggcagccgc taaggcggtc cgtgtcatgc
    88321 agcaggagtt tgcctccgat ctggcccgat ccgtggccgc gcatgtgtat ggagagatgg
    88381 atctgccggc gtcggcctcg acgccgtccg gttgtccgaa agaggcggtg cagccgccgg
    88441 tcagtcgtta acgcggtgag tcaaccacag gagagaggag cagggatgag taaaataggg
    88501 acccggaagt cgggtgagca aggcaacggt ggacgccggt tgtccagtca ggcgatgaag
    88561 aagcggttgc gcgaacggct gacggcagat acgcagcagg tactgaagag cgacatcgtg
    88621 agcatttttc gcaaacaggg ctactacgag gaattgccat tggatgagtt gcaggatcgc
    88681 ctctccaagc tccagtcgcc gttggctgac agtctcttga aggggagggt gtaggatgcc
    88741 gtattactat ttcgactcca cggcgttggt gaagcgatac agcatggaac gcggaacgcg
    88801 ggtggtgaac aagctcatgg tgaaacgggg caaggtcgcc attctgccta tgtggagcgt
    88861 cacggatttt tattcggccc tctccgttcg cgcgcagcag ggtgaaatca ccagggacga
    88921 ctgttattcg gtgctgtaca agttcgagat ggaagcttcg caggggcttt tccaatttat
    88981 ctccccgacg atggagacct atctggctgc gaaggagttg attctggact atccggctct
    89041 gcgttcgccg cagttgctac acctggcctt ggcgttggaa ctcaagccgc tccggctgac
    89101 ggtcgtgagt gcggataagc agctcctggc ggcctgtcgt ccggcggggt tgcatatcat
    89161 caatcccgaa gacgactagc ggtggcagga tgcggaaaca gcccgccagc ggcgttctcg
    89221 catcgctcag aggctcaacg taccgcaagg gtacgcctcc ccgcttcgca ttgctgcggc
    89281 cttgctggac ggcctgtttg cgcatcctgc agacatacgg cccatcgaca tgcctttggt
    89341 ggaggcagtg agtgttcccg cagggtgctc atgaggctga gcctcggagc aatgcgagaa
    89401 cgaagcaagc ggtctttttc aacggatggc tggcccggat tattcatgaa tgccgtcgcg
    89461 aggcgtttga gaccgaagcc cgccggggct tctcgcaagt gaggccgacg caggcgtacg
    89521 tgcagtccgt cgaggaggcc gaacgagaac agccccgccg ggctagaggt tcggacgccg
    89581 gagtaggtat tcatgaatag tccgggctag gaggggcagc gaaattcccc ttgtatcgaa
    89641 tccaacacaa tcgaccagtc ttccatactc tgctgcgccg ccttccgtcg aggggaggcg
    89701 gtgttcttcc cctccgtatc attcggcccg ctgagagctt cctgcgcatg gcgcagttcg
    89761 agttgtgcca gttgaagact gccgcagacg ccggctgaag cggggagttc cccgccggct
    89821 cgccgaaaca gggcgatcgc ccgatatcca tgttcctgcg accggtagag gtgctccgtc
    89881 gcattgaagg tctgccgcgg ggccggctcg gtctgccgct tggagagttg tgccgccatc
    89941 aatgccgcgc gtcccatgct catcgccgca gtctccgcat cgtcattacc catggcatct
    90001 tccgccttcg cccggaggcg gtcgagttcc gcctcttccc ccatcacctg agcctgcccg
    90061 ttgccggcga tacagacgat gagcgccacc gtcaggatct gacaggccac gagggtccgc
    90121 cgcatggccg acctaccgaa gcagaggaag aaggtggcgt ctgctattgc acgtgttccc
    90181 aggtgtcgat ctgtttgatg ctccaattga cggcttcctt caatttgacc aggttgggat
    90241 cggcatcgtt ttcgtgcacg cggtagagcg atttttggag cgtgaacaac ggttcatagg
    90301 cccgcatgtc cggcagtttc ccgagcgccc aggcgacctc ggttttcacg gcgacatcct
    90361 cggtattcaa cgtttcgagc aggggatcca cgatcgtgaa gtctccgtgc aaagcgccga
    90421 cgatgcccag ggcttttgcg gccggcgcgc gcaaatccgg ggggccggat ttcatcgtct
    90481 tgatgagcac cgggatcgtg tctttctggc cgatattgcc cagcgccagg gccgaggctt
    90541 tggcgatact ccggtcggct ccgttcagtc cgtcgaggag gcgagggatc gccttcacgg
    90601 agaacaggtc gccgaacagg gccaccagct tcaccttgcg cgcgaccgga gtcttgggat
    90661 cgtcgtagag gcgaaacagg cgcgcttcct gtccccattc agcggtgatg agtgcgggga
    90721 aatcgtactc ttccagtcgc gcctgtaact tggccatcgc gtaggccgtg ggatcgccgg
    90781 ctttcgcgag cgtggccgcg gcttgggcgt cgtcgaaatc tgtccggacc tctttgcgcc
    90841 aggccatttt tttcccgttg aggagatagc cgagctgaca ggccggtcct ttccagattc
    90901 gtgacggcgc atcgggaagg tcggcatcgc tccccatcgt ggtggtgccc tcccaggtct
    90961 ttcgctgctc gcatttgatc ttcagttcca catcgtgcgg ttgggtcgga tcagtcacgg
    91021 tcgtgtagtc gaattctttc atgcgggtga cgaccacgtc gaccaggggt ttggcgtcga
    91081 ggctgccctt gtccgagagg acaatcacat cgaccaacac gcgatcgatg cgttccagtt
    91141 gactcttttg ttcagcggtt aaggcgtcgc ggcgggccca actctggtcg cccaggagta
    91201 ccagggcccc cagggtgatg agcgaaagga tgcatctcat gtctgctcca ttttgatgaa
    91261 aagacgatga tcacgaaccc tgccgccact ctatacaacc ccttccggct gttgcaagtt
    91321 gcgcctgtcg gagagccgtc cgtataatcg cgccatgttg acggggacac gaatcgcgtt
    91381 catcggcggc ggcaatatgg ccgaggcgct gctggcaggt ctcctgcgca agggcgtggc
    91441 tgtcgccgag cacctcaccg tcagcgatcc cgatgtccat cgccgggaac tgctggcaga
    91501 gcgattcggg gtcacggtcc atgccgacaa ccggacggcg gcgcaaggtg cggagctcgt
    91561 cgtattctgt gtcgaaccgc aggtgctcga ttctgttctg gatgaggtgg cttcaagtct
    91621 ccaatcccgg cccctcgtga tgtccgttgc agccgggtat ccgatctccc gcctgcaggc
    91681 tcacctcaag accgcaacca gactggtccg tgccatgccg aacacgccct cgaccatcgg
    91741 agaaggggtg acggcgatga gcatgatgcc gggtctctca tccgaagatc aggaccgggc
    91801 acgatgcttg ttcgaatcgg tcggtcaggt ggttgtggtg gaggagcggc tgatggatgc
    91861 cgtgacaggc ctgagcggaa gcggaccggc ctacgtgttc gccatgatcg aagcattggc
    91921 cgacggcggc gtgttgatgg gactacctcg aatcaccgcg caagttctgg cggcgcagac
    91981 agtagccggc gcggcgcgta tggtgctgga tcaggggaca catccggccg tgttgaaaga
    92041 tcgcgtggct tcgccgggcg gcaccacgat tgcgggactc tcccgcctgg agcaggggcg
    92101 gctgcgcgcg acgttgatgt cggccgtgac agccgcggcg caacgatcgc aggagctggg
    92161 gaaggaagag aaccatcacc tctaacaagg agcgtgtctc gtgcaatatt gggtgaaagt
    92221 cgtgtttgcc gacaatcagg aactactggt gaaggatgcg attcgtcaca cgatcagcga
    92281 agacatggag gtgttggaag tcgattcggc caaagaagtg atcatcgtcc ccatgaaaca
    92341 gattaagtac atcgcttgcg atgccactgt attcgctcaa aaggcgaaag cataggttct
    92401 taccttttcc ggtcacgttg tcggggcggg tgatcgtttc acttcgatgt ggattcatct
    92461 cctgctccct ggagtgactt ctcggtccgg gcgtccatat cccttcaaca agaggtagag
    92521 aagcgtcgaa ccgccggcct gccgacatag gacgactagg atgggacctt aggctgaagg
    92581 tccggatagt cgacccaggt ttctttttcc gccagatacc gcgctccttt gaagttgagg
    92641 cgtgagcgta atcgtgtgaa cccgaatccg agcagcttgt cggcgacagc cctgactgct
    92701 gcgcccgtgg tcggttgatc ggttccctcc acgacgaagg cttcgtagat caccttcccc
    92761 gcatacccga ccgaggtccg gttcacatag actgtccggt tgaaggagcg gtcgctggga
    92821 tccatccctt ccatctggta ccgttcgatc agcccctgat tcttgaagcg aagcggcagc
    92881 ccattcatgt caaccctcca tcgatacgtg aaacaagtac tctggaagac agcgtggagt
    92941 gagtgatgca acggcacaga gggcgagcga tctcgcagga tcatcggtcg acgagcgctt
    93001 cgaagagagc gttgtaatcg gccttggctg aatcgacgaa catgcacttg tctccccgac
    93061 acagacggag taatggccgc ctccgaactc cggccctgag caggtgtcca ctgcatatgg
    93121 ctcaggtgct gaagcaaact gctcattgcc ctgaacagca tgtggaccgt gccgaagaac
    93181 gagccgccga ttgagccgtt tccgctttca ctggtccgtc actatcgcca aagtatccgt
    93241 ggggacattg actcaggttc ggtatacgac agctgcgggc gagaaacggg gtcggaagag
    93301 gaagcataac cgcctgatgg agcgggcgac ccggggccat gataccactg actcgttccg
    93361 caccgtactg ttgcgtgaac caaatagttg tccggcagga ggctcgcccc gtcctgcgaa
    93421 ccgaagtgta tagtgagttc gagccatata ccgagacgta atcgcatctg agtcaccctt
    93481 cagttattgc aaatctccgc ccatggcgga tagccggcgg gaccttcgtt catcgccccc
    93541 gtagcgcatc ctgggagtgg ctgtcgttct ccaaggctct gcagtcattc cggtgtgatg
    93601 ctcgatgatg cgccggagcc tctttaggaa ctgagcaatt ttgaccagtc acaattcggc
    93661 ccctcgggta tgttggaaaa aatttcaatg atttgctgtg ctgtttcggg ttcaaaaagc
    93721 catgggtcct catcgccacc tcatcccggc ccggctggct gcctgttgct acccttcgtg
    93781 aggagagaca tatgaactgc caaaaaataa tgaggaagtt cacggctaat ggtggttcag
    93841 tccggggtga gagccagggg cgctatcgca ccttgttcga gctggccccc atcgccgtct
    93901 attcctgtga cgcctcgggt ctgatccggg aatacaacca ccgtgcagtg gaactctggg
    93961 gtcgcacacc ggcgcacgga cagactaacg aacgtttttg cggttgttta aagatgtatc
    94021 gcccggacgg cagttttatg gcccatgagc aatgtcccat gagcgacgtg ctaagcggga
    94081 aagttccgga gattcatgac gcggaagtgc atatcgaaag gcctgatggc tcgcggatca
    94141 tcgtcattgc gaatattgct ccgctgaccg atgaccaagg agctgtcacg ggagcaatca
    94201 gttgtttcta cgatattacc ggacgcaaac aggatgagaa ggccttgcat gtgtccgagg
    94261 agcgctaccg gcatctctat ggccagttgg agtcgcgcgt tgctgaacgg acggaggagt
    94321 tggccgaatc gcagaaacgg ttgcgagtcc tggtcgccgc actggacgtc acggaggagc
    94381 gtgaacgtca gcgtctggcg gggaatcttc atgacgatct cgcacaattc ctggcactga
    94441 gcagaatcaa gctcgctctg gcgaaacagc agcccatgca ggctcccctc gcgaacctta
    94501 tcgctgatgt ggacgaggct actaacaagg ccatggtgta tacgcggacg ctgatttctg
    94561 agctcagtcc acccgcttta tccgagtccg gactaccgat ggcgctccaa tggcttacgg
    94621 agcaaatgcg gcaacacgat ctctcagcct cgttggagct caagacacag atgccgacca
    94681 tacccaagga tcacgcgctt ctgctgtttc gatcagtccg tgaacttcta tggaattgtg
    94741 tgaaacatgc cggggtgtcg aaggcagcca taacgctcga gcagctcgaa cagtcgttgt
    94801 acattcacat ttcagaccaa ggcgtggggt tcgatccacg gtccgcaagc gagacagaat
    94861 attctccgac tcacgggttt ggattgttga gcattcgcga gcgaatgttg tcgctgggcg
    94921 gccgtttagt gttggagtct tcgccacgga acggcacgac ggccacactc gtagtaccgc
    94981 tgcgccatgc tgccgtggaa tcttcgtccg gtcgtgctcg atggtcgggg ccgggagtag
    95041 acggagggga atattgagcg tggcggacat ctccgccatg gtccttgagt cgatccttcc
    95101 ttgcaggctt cattacgaag gaagactacc gcactttcac gagacggtcc atccgctgtg
    95161 aaagcaggaa ctggtctccc tcttgtaatg tgtcggacta ggggagaacc aagaaggtaa
    95221 acatggaaaa gcgaggcccc ggggaggaat attccccacc gggacctgta cggtgtcagc
    95281 gactggattt agcggcggtt gtcttttttg cagtccggtc cggtttggca aggagtgcca
    95341 gcttcctttt cgtcaccttg acctcgagcg catcccaatc gatgtcatgg tgttccgctt
    95401 tgggcagcat ctcgccctct tcttcctcga tgtgatgctt cacgagttca ctcagcacac
    95461 aaaacttagc gtgaaatctc tcgtcgctcg gcttcagctt cttgagttca gcgatcaaaa
    95521 cgtgcacgag atgatgctct tccaccgctt cattcatcat gtcggcatcg tcgatggcct
    95581 cacggatggc cggatagatg agtgcttctt ccagctcggc atggatctcc agctcgtgaa
    95641 tcgctttcag cgcgatggcc gcttgctctc cgccctcagc agactcaaac tcgtcgaaca
    95701 atccctttac cttcttgtga tcatctttca gcatttcgac tacggcagga accgttgcac
    95761 tttgcttggc catatctatc tccttactct ttgtaaaaag ggggtgcagt ccagggagct
    95821 gcgtgtgtgg gcgacttcat cttacgggag tttggcgacc aagctactgg gcgaagccct
    95881 tgcaggactc ctaccagaag gtcctgggcg gcatggtacc tgcgttgatc aacccgttaa
    95941 tgagtcgagg gactcccttt gtccgagtgg ttgaaggaga catccgccac ttgctgtgct
    96001 tcgtcgatga gtaagacggc agattggccg cgagagacct tgctgagtcg ttccagaata
    96061 agcggccgga cctcgaatga ttgctctttt ccgtcctcgg tacggatcgc gatggtcttg
    96121 tcttgcaggg ccttcaagat tactccgcgc acctgtcgga gattcccttt gattggtgac
    96181 tgtttatgtc cctgcgtcct cgactgacca gctctgtggg gtgtgccgag cgtgacgtct
    96241 acgattgtgt ccaactcatc gaggagaaag gtgacctccg ctccgacagg gagtgaggac
    96301 actttgcttc tggccacagg ccgaatacca tgggactctt ctttgccctc cgcggttcga
    96361 atgactgccg tctcgtgacc cgttcccagg gtcacggcga gctgacctcg aacaattcga
    96421 tgatggctga tctcgccggc tttatgcaca tccaccaaga gattctgatc attgaccgta
    96481 atctctacca gatccccgat gcggaggtct ggtagtccct tgtctttacg cacattcatg
    96541 gggacgaagc gcggctgtcc ttcgccggtg ttcaccctcg cctggtcgct tctgacttcg
    96601 tcgaccgttc ccaggaacgt tcggttcccc tgcagatgct catggtctat gtgtttctct
    96661 tcaggtgcag cgcgggcccc ggcaacgggg agcatacagg ccaaggcgaa gatggctacg
    96721 gactttgact ggaatgttgt catgaaactc tccatttctg gtcaatcgtc atagtggtca
    96781 atggaatccg gccctgaggg gagtgcgttt ccggtatcgc ggggttggtc gacctgtcta
    96841 ctgctgtcgc atggtgatgt ctcgctcggc ctggagccag tgttggagat tggagccctc
    96901 atggcgatcc cccgcctcgt aaagttcata agcccgttgg gcgatggctc cgtgagcggt
    96961 cggacgtgca ctcgcggcgc ggttttggag acaagacttg actgcggctc gcaattcccc
    97021 caggtcgtat cggccgttta ttttgacagg gaccttcagg acacggtcca caccgttcgc
    97081 atgtgactcc gtgagggcgg atatcatggg cggcgacgat aagacgatgg ttctctcgac
    97141 gtagccgcga tcccgtatca tcttcaaatc gtccggtccg ctcggatgag atacgtgtag
    97201 gtccaggatg ataaggctag ggcgagagtt ctcgagcatg gtgagtatgt tctctcgatg
    97261 cgcggggatg gcgaccttat ggccactctg ctggagagcc tcggttagtt gttctctgag
    97321 ttcactgtcc tgcgtgagaa gcatgatcat ggtagagcct ttcaaggacg ttaggagcga
    97381 gtggaccagg tgcaagcgac ccgttgtttg tgctgcgaga atctttccca agcccatcgc
    97441 gagggtgggg gttggtctcc atggcgcgtc tttcattccg gtcctataaa caagtatgcg
    97501 ctggaggcca tttcaggact gagcaatttt gaccagctat actccggctc agggcgtagc
    97561 ataggcagct gggattggcg atgtcgttag attggtttta tggcagggcc cgctatgaaa
    97621 tgtgaaccat ctgggccccg atggaggtgc aagttatgaa caagcaagtc ttcgcgaagc
    97681 gctcgttttt gggcgcatca gtgctaacgc tcacgttctc gctgtcgccg ttcctcactc
    97741 atgcagagga gtcgcaggtg aaaggcgcca tgattaccgt tcccacgaat acacaaagcg
    97801 atgtggcaca gggtgctgta gaggactccc taaaggcctg cctcgcgaga attccagata
    97861 aggccagtgc cggacagcgc atgctggccg aacggacctg ccaaggagag gaagccatca
    97921 gaaaaacgac tcacggcggc ccgcagtttt aatactcatt gtctcgcgag acaatcaacc
    97981 tgcagaggag tgtgcaatga atcaagaaca atttgagcaa ttctggttac agctcaagat
    98041 gcctttaaaa gccaagtggg aaagaatcac tgatagcgac cttggagaaa tccagggcaa
    98101 tctggcgacg tttaaagacg ttgttctgaa gcggtatggg gatacaaaca cgagcgaagt
    98161 gagtatttgg gtcgatcggc ggtattcgca ttggacgggg aactatctcg gatatcgtga
    98221 tcctgagccg gcctcctctt aatcccctcc gctatcggcc gggttgagcg gcctggtggt
    98281 ctacccccgc acgcctcacg cgaggtacag aaagagagtc cgcacatggt caaaatcgtt
    98341 atcaatgcaa gttacgaccg gttctgcgtg agccataagg cgtttctcag gttgcgagag
    98401 ttagggcaac cggatgccct taatgaaacg gatcacgggg cctattggcc tcaggccgcc
    98461 agccctcaag agccaagcct caatcgatgc gggatactga ttcctcgcga tgaccgccgg
    98521 ctggtccaag tcgtggaaga gttgcgcgga gaggcgaacg ggcactgcgc ggaactcaag
    98581 gtcgtcacca ttcccgatga cgtggaatgg gtcgtggtga aaaaagacgg gagagaacag
    98641 gtgagtgagc agcaccgcac ctggagttga cagcgtggcg ttatcaaagc ggtcgttcct
    98701 gtcggtggct cgaattgtac cgttggtcat gcgtgatttg ctcgccatgt tttgtgccat
    98761 tcgaggcgaa cccatccctt atcggtctcg gtcagagtat gccctgaaga ccgatccggg
    98821 cgaccatttt catggaggat gatatgaaga cgacaccaca gacatatgtg ctgaccgtac
    98881 attcaccttt ccgtagcgct cttcggatct ggacggggag gggcctccat cagctccgtc
    98941 actgttcagc caagcgaatc gaccatgtgt tgtcagagat gcaagcgcac ctggacgatc
    99001 tgcggagttc gctgaggccc cccgttaccg caggaaccaa ctccttgaat cgggggcgtt
    99061 gatgccgcgt cgccctcacg tcggccactc aaagagaatc ctcttcgaag ggcgcgccgt
    99121 gattcatgtc cagcacgatc gggcggaaga tgatcaggtg gttgcaccgg aaagccaagg
    99181 ccaatctgtc gccttcggcg tgcgagcatc acagacgtca cgcatcgaag ctatcatggc
    99241 tcatatgatc tagatctatt tcaagtgcat cagaggtggc actatcgcat cgtgaaagtg
    99301 agtgcgaaga ttggccctga atcggcccat gtggcgtatg acagcaggac ccataatcaa
    99361 ccaggacatg aatctcagtc agtggtacgg cgggttgcgc tgtcccataa ggcaacgctg
    99421 gtggccgtat ggaagccggt tctcccgaga accgactgaa aagcgggcgg ggcatccgtc
    99481 cgggatcctg ccggttgtca cccgtagaac ggtcgctgga gaggaggcag ctgcttgaag
    99541 gtaagacacc acagcacgag gtgcttcttc aggccgatgg cgtcaaaccc ggtctcctgg
    99601 cagaaagggc agatcgcaca tcgttggtcc atggcttcac cgtctgcggg acgtgtggtg
    99661 gtcgacatct ggtagcagcc tccctatgct aatggttctt aacctgcagc ccttgttctc
    99721 agcgattctc cggaaggccg tggtcttact gggcggtaca ctgagttaaa gcaatacgtg
    99781 cgggaaatca aatggtccaa tcgccaacgg tcacggccga actttcttgt tcgctctctg
    99841 ctggtacgta ctagaccccc aatttgccgc tgctcccgat ccctcttggg gtgtgacgtg
    99901 ccgcgaccta ccgagctatg tctcatggct gcgcttgaat aagaggggcc gctgtgtcac
    99961 agttgcgccc cagcctgggc cgagattttt acgtgttgag attttcctat ggccgccaaa
   100021 cgacccacga aatttaatcc caagaccttt ctgggaaaag tagggaccgg caaaacgaat
   100081 cttacgctcg aaaaacatca cacgattttt tctcagggtg atgaggcgga tgccgtgttc
   100141 tttgtcaggg ccggcaaggt caagctcagc attgtctctc agcaagggaa agaggcggtg
   100201 cttgcgctgc tggaacccgg cgcatttttc ggggaatcct gcctcgccgg acagatcgtg
   100261 cgcatggcaa ccgcggttgc cgaagaggca tccgttctgg tccggattga aaaaaccgcc
   100321 atgattcaag tgcttcacaa ggatcccacg ttctccgagc tgtttatgtc ctatctactg
   100381 ttgcacaata tgcgcatcga agaggattta gtggatcatc tctttaattc cagcgagaag
   100441 cggctcgcgc gggtgttgct cttactggca cattttggga aggacggcaa gccggagtcg
   100501 gttgtaccaa aggtgagcca ggagacgttg gcggagatga tcggtaccac tcgctcacga
   100561 gtcagtttct ttatgaataa atttcggaaa ttgggattta ttaaatacaa cggcaagctt
   100621 cacgtccaca gctccctcct caatgtcgta ttgcatgact aggttgcctt tttctcgatt
   100681 gccgtcactc cttacacatg ggccgaaata ggttttacct ctgcactcgg cattgttgtt
   100741 gctgagcagt tttgaccagt ctcagttgcc ccagccgcgt agtgtgggac aggtcataga
   100801 tctgtccgtc ctactgcttc cttatggcga cgatctcatg attgatcgtt ggtattgctc
   100861 aggcaatgag gagattcgag tatggcgtgc cggattgccg gggcgattca gtgccacctc
   100921 cgcatgaatg tatccctgtg ctgagtctgt ggccaggcga atccggagat gcccgcgacc
   100981 tctcgaatca gggtaaagtc catggtgtga aatggggaat cgagatgagc actcgtgcca
   101041 ggtggatcag tcttcgattc ggatgctcct ggcatcctat ggccggcgga tactcccgga
   101101 agtcacctga ggatgggatg aggggaaccg cgtgccggtc aaataatgcc ggaggctgaa
   101161 tttgcatccc cgtgggcggg acgtggggta ctgtgttgac ctgtgtgtcc tctggccgtc
   101221 tgtcttgttt cctgctgcgg acaatgggtt cgcacgcggt atcatgatct acataagagg
   101281 cgaaacatca ctatggcatc caaccgcaca gccggtttca atcccaagac gttcctagcc
   101341 aaagtcggga acggaaaaac gagtctcgtt tctcccaaac ac
//
LOCUS       DAORRQ010000006        93479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_6_length_93479_cov_970.229320, whole genome shotgun sequence.
ACCESSION   DAORRQ010000006 DAORRQ010000000
VERSION     DAORRQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 93479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 93479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93479
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6_length_93479_cov_970.229320"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            613..1461
                     /locus_tag="PK224_03250"
     CDS             613..1461
                     /locus_tag="PK224_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HRB14784.1"
                     /translation="MRRLAYTFLAFCVLVPCSSLYAWDTSGHAIVAMLAEERLTPTAK
                     AAVSDLLQGHPLVDVASWADQVRNQQTAPWHYVNIEIDETQYDTHRHCPDRQCVTEQV
                     ERFRATLADDSLDPMKRQKALKYLVHFVADLHQPLHAGQNHDRGGNDAKVEFLGRAVN
                     PFTKKPWNLHQVWDNGLLDQYAPNAKQAVIQIDQYLASQNEAELAIGSVIQWAMQSHD
                     HARDHAYRFGTDKRLNEQYVKQALPVIEAQLARAGVRLAFLLNTALRTEAVDSVVGEG
                     VSSPIQ"
     gene            1473..1820
                     /locus_tag="PK224_03255"
     CDS             1473..1820
                     /locus_tag="PK224_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14785.1"
                     /translation="MSDSLTPRETFTTEEGVLANMWAIEALVELLERKGFCTKQEVHD
                     LIAELRVRHAELNGKVIVPGPPANTKEEQYLIDHVLKVIEAVGLQPAESKELLRRVSL
                     IIDQQARTKLKPN"
     gene            1832..2035
                     /locus_tag="PK224_03260"
     CDS             1832..2035
                     /locus_tag="PK224_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14786.1"
                     /translation="MGSPSNPQDGQPFPVPYLCTEVSDRLIQQILDAFNREGLTAEQA
                     RQLLGRVKHLLDAGELLARRTSH"
     gene            complement(2219..2635)
                     /locus_tag="PK224_03265"
     CDS             complement(2219..2635)
                     /locus_tag="PK224_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HRB14787.1"
                     /translation="MFCLVYASSATRPFSPSQLTDLLASCHANNSQLGITGILLYKNG
                     NFMQALEGEEQAVLNLYAKIFEDERHTGILTLLQTTILARQFPNWSMGFRDLNSPEVR
                     ALPGYNEFLNRPLTGAEFSADPTYCQKLLTIFKKMM"
     gene            3580..3933
                     /locus_tag="PK224_03270"
     CDS             3580..3933
                     /locus_tag="PK224_03270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB14788.1"
                     /translation="MHPRYNDRKPASAKATLSAGPRVGEGQVLDLTNPGCLLHTAVPL
                     ERDQAVQLVIYLDVLRPMRITLGIVRWVSGKTAGIEFIRMSSEDQARLRTFIGHKPQL
                     RVSGRWGETPLCVAY"
     gene            4149..4619
                     /locus_tag="PK224_03275"
     CDS             4149..4619
                     /locus_tag="PK224_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AP2/ERF family transcription factor"
                     /protein_id="HRB14789.1"
                     /translation="MLNIRRVDHDASSTHCWRVKVQRRKQLFTRDFSDGLHGGREAAL
                     QAAQAYRDMLVRTQAPLSKPDYCAILKKNNRSGVSGLTRVDRWELLRGRRVRKVFWGV
                     QWPIGNGRSRHKTFSIMKYGEEAAFQLALAARETALQALISQTFSPFACRDGKS"
     gene            complement(4864..6672)
                     /locus_tag="PK224_03280"
     CDS             complement(4864..6672)
                     /locus_tag="PK224_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14790.1"
                     /translation="MSKSFGLNQLLIATSLALLTLTIMSPEIFASHEKLVDRHGQPAK
                     PNGACTTPLLASDNWSSTEKSIWAKICIGDDADLGEWNNNEHVDNSFRVVSPRFLETI
                     ILHQPYSSAITHYGVTIRGAWFKEIFDLSHAVLANPIRLENCKFESTVIFTNLRSSQL
                     ISFEDSKFNDDLDMEQIHTDSTVSLSGSHFSGDVDISNANIRIGLKMVDATFMKPLDM
                     RGIRAGAIIGRNSRYTDIELLGGSKIERNVELDGAEVSGVLDIRALEVNGNIFLRGGG
                     KFNRVIIRSTKVQGGLELDESAYKGHVEMTGLQTNGGVSVTGITGTSMKISNSSIGGD
                     LNLIASSLTSLSLSETSIRGEFVLAGSSFKSALHWNKNGRISMINTDTGAFRIYYPDD
                     NEKAQAVIPSKVGLTGFTYSRISGTANTDIAKGNAPELIGADAMGKWLLGQLPYSRQS
                     YQHLANVLRTSGYVEKANDVLFESRNREYYVAEGLQKVSLWLEWVLIGYGYRIYLSFF
                     WAIGLILVGATILWISRQGVAKQIPFARYPFGYLIYGLDLIFFSFDMLLPIIKLREQH
                     YSIDLVGWVRYYFYFQKVAGWILGSFIVAGLSGLTK"
     gene            complement(7109..7924)
                     /locus_tag="PK224_03285"
     CDS             complement(7109..7924)
                     /locus_tag="PK224_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRB14791.1"
                     /translation="MPKTKVFLTWSGRKSKQLSQALRDWLPQVIQNLEPWMSDSDLDK
                     GSRWATEISGQLEQAQIGIICLTPDNIDKPWINFEAGSLSKQLEHSRVCPYLLGLSPS
                     DLTFPLAQFHATIANKHETLKMLQSINKMMGKHSLSEQSLDSSFAMWWPKFEEDLHRV
                     TQYSEDIPAPPRTTENMLEEILNAVRAQSRWHTDVIKILEGIQGSPQSASSFFSRFSS
                     HLTGNKIRLPKEDWWKLAEILGCIEAQMNLSGGISPNLAEDVSGNDTTGTEIL"
     gene            complement(8425..11721)
                     /locus_tag="PK224_03290"
     CDS             complement(8425..11721)
                     /locus_tag="PK224_03290"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRB14792.1"
                     /translation="MILKTPYALTLDESPLKNDLQQIGDPSRESEGTLTPFTTNNVRG
                     WLFQPTHGNESLFIVLPKTNIEKVPKEHVRIIAAHFSEGSTSVADLIEGKWLRHPMIV
                     EGQHRDHAVEHRKVLDSWKSAFTYVVAKTDSSVIGLRPPQIGAVHAVHAHWSVTEAPA
                     TVVMPTGTGKTETMLSILVSACCERVLVIVPTDALRTQLAKKFLTLGVLKLQDANILA
                     PSALHPIVCVLQHIPKTSDDVKLLFSPAHVIITTSSIVGSCPPNVQQAMAKYCSHLFI
                     DEAHHTKAPTWNTFKNRFSGKRVLQFTATPFREDGKPLDGDIIYVYPLKKAQAEGYFK
                     PIRFLKVVEFNPKQSDNAIAKKAIEQLRTDYSKGHILMARVETVSRAIQVAEIYKRLA
                     PDLEIVQLHTGIKSIRAREEARRKILTKTSRIVVCVDMLGEGFDLPELKIAAFHDIRK
                     TLSVTLQLAGRFTRSRPDLGDATFVANTADLSVQDELRKLYSRDPDWNVLLPQLSDAM
                     IGEQQSLQEFLRGFSDFAKEIPLKTIRPALSTVIYRTQCDNWRTDNIQSGISNIASCE
                     QVHVATNEKEHTSVIVTARRVPLPWTDIVKVFSWEWELYVIIWWPEKKLLFINGSTNA
                     GEFKSLAQAFAGDDVRMIKGQVVFRSFHGVMRLRLKSVGLAEQLGRNVSYTSRMGSDV
                     ATVLADAQRRRTRKSDLSGTGFEGGATATVGASRKGRIWSHRRDRIQQLVEWCKHIGG
                     KVIDSTIDPDAVLSGTLETKVVATRPEGVPVVIDWPEEIYKSIETPWSISMDSTMFHI
                     SEVDLELVNPTLSGPIKFGITSGHAHVEFELEIYRLDETSDFRFKPLSDSAVHIIKAE
                     TAIPAEDFFTSNPPRVWFADGASLDGNEYTPLKTVFPPYSVEKLVDNWDWTGINIQKE
                     SQGDAKDQDSVQAAVIKRLKGNSYHLIFDDDGSGEAADVVAATVIGSFDAPEQIDVEL
                     YHCKYSKKAKSGGRIDDLYVVCGQAQTSIRWMSSGEKRSDLFTHLLRRESQRQARGAS
                     SRIERGDFRLIEMLREMSHTTRITMKIFVVQPGVSKAVISEPQLRLLSVTENYLTETY
                     QLPFRVIVKP"
     gene            12406..12717
                     /locus_tag="PK224_03295"
     CDS             12406..12717
                     /locus_tag="PK224_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB14793.1"
                     /translation="MEHTWLTVDELAAVLKVSPKSIRRAYRKGEIPVDRFCRFVRFDL
                     ERVKEALKAKGHDLTVVEAGKNVGQRSATGGESRRRAQRPSPRLGKTGASIAQTPRRK
                     K"
     gene            12714..13715
                     /locus_tag="PK224_03300"
     CDS             12714..13715
                     /locus_tag="PK224_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB14794.1"
                     /translation="MTVSGGFTQTIDWLAFTLPKAEVADVITLIGGDWFQSESGFRGY
                     PVAQLMTQGKTGVGKLGTGAPRNPKEVHVDLSAGIVSQWDETKLKTVLAWIFAQKGHV
                     TRIDVALDDREASVAVETVRLAVEAGQVVSRSKQFKVIQASNHREGVRTGETLYFGSR
                     ESQTMLRVYDKRLELQSRGREDAALYGVRWELEFKQDRAQACAKALLTLDPEDWRAFL
                     VGVLRSYVDFRETTREAESYEKYRAPLVGWWKALTEGFMRCRLVVERIQQRLDDVAAW
                     LANAISPMLAVVVACRGDQFLMEMIYAGTKRWTQKHYALLKQRRRGTPYVLHLKPRT"
     gene            13922..14185
                     /locus_tag="PK224_03305"
     CDS             13922..14185
                     /locus_tag="PK224_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14795.1"
                     /translation="MQVKAEGAVQGYVERRSREGKVYRSVDFYVKGKDPGVLRLGIPD
                     DQMSLIEICKQAEGKQAKASIEVRKFEQTGRTFFDLTGLDVVK"
     gene            14278..15546
                     /locus_tag="PK224_03310"
     CDS             14278..15546
                     /locus_tag="PK224_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14796.1"
                     /translation="MKLFTLTSYLSLIWGLLISFCLAPAESLAQTATQYSRVVAQAER
                     IAYLAAQRSALANQVATAALAPSAASMAVRMVAGPVGWAALGVSAGLVLAQMYYSQSD
                     LSAVKTAASTPGGWQVTSSNAGVQTFPGFGTNTPGNPAYPNATIQFSATNVPLCAVDT
                     EYQHDWVVGPFQSLSTAVFFTGNFFVNGPAVGGYSLYVCHRKGIPGSTSPIQEGLIPP
                     TQQQVANFVGGLPASDPKSVEAHTNPVGTNGSTQPADTTISQAVTPTEMPTTVKPKPV
                     PAGDIVVVDTVPPPASTPQQNTQQQTTTTTTTTTQNPDGSTTQHEETQATTSCAVGSH
                     ESRTFGTVLQVHQTIWATSGLLGTLNLLKSLTWPSTLPVIALPSAFFGSQQVDFNQWA
                     WFFTVLRSLVIATASIAAYRIIFVGGGQTT"
     gene            15543..15818
                     /locus_tag="PK224_03315"
     CDS             15543..15818
                     /locus_tag="PK224_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="HRB14797.1"
                     /translation="MTAILTLIYCWLQEFFFSLTDWGLSIWDSLLSVADSTLATIGTA
                     GLTLPVIPDQYAWVLGATGMSQALAIVASAMGTRFILQTIPFVRWGS"
     gene            15898..16902
                     /locus_tag="PK224_03320"
     CDS             15898..16902
                     /locus_tag="PK224_03320"
                     /inference="COORDINATES: protein motif:HMM:NF017518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HRB14798.1"
                     /translation="MIELYEGVPGSGKSYHAICEKFLPWVKQGRRLYIAVDGIYLDRL
                     SLFTGIELSALEQQITIWKDSVEVLQAFPHVEPGSAVIIDEAQTVFRSMQKVEPGLLR
                     WLETHRHYGVDILLMSQDFRQMSQGVTRLIEATVKFRKLSFVGLSKKYQGKVRGNPED
                     HEVIRTFVGTYSPAGYAYYSSYASAAIREEKRSHTVFKSARVAIGIAAGLFAIGLMVW
                     RPWSSLSASKPTTAAGSAVLPTTIGAPASGSASLLNPFGMSPPAPATPPAPKRPVRIL
                     GGAGMSKDRQGWRYLLDSGEILTAAQITGRYGLSVSEVYEDGSMRLIGKGVFYGPAGD
                     "
     gene            17063..17275
                     /locus_tag="PK224_03325"
     CDS             17063..17275
                     /locus_tag="PK224_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14799.1"
                     /translation="MKGRSWVKGLGGVLMAFVLALGLPALSQAQLFPVSADVATVRAD
                     LLLWATALIGVALAIYAFKRVRAIVG"
     gene            17368..17496
                     /locus_tag="PK224_03330"
     CDS             17368..17496
                     /locus_tag="PK224_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14800.1"
                     /translation="MTAQQIQTIGNALAVDLVAWGTAAIGLALVAAGAAWVLRLLR"
     gene            complement(17493..17996)
                     /locus_tag="PK224_03335"
     CDS             complement(17493..17996)
                     /locus_tag="PK224_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRB14801.1"
                     /translation="MSVDSSIGSSNGGWPLSPVRKYAVPRYRVTLVREGRAMPAAESV
                     HTSEGAAAILRPLFAGLDREQFLICGLDAKHKLIGINVVSTGSLNLTVVHPREVFKPL
                     ILMNAGAWLCSHNHPSGDITPSPEDRVLTKRLREAGELFGITLLDHLILAEERYYSFA
                     DQGWPGA"
     gene            18555..19922
                     /locus_tag="PK224_03340"
     CDS             18555..19922
                     /locus_tag="PK224_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB14802.1"
                     /translation="MNIKRAQTPRGTCSILDYRYGGIRYRPVLGYDLTTEEEQAAAIE
                     IMVAIQVNVGIQAPQAQPARPSLSFCEFAPRYMAYLEAKKLMALDRPLTILNLHLVPH
                     FGSVPLKKLTMDHGLTYLAKRRAEGAAEGTISRECGVLQAALNYAVEIDALDKNRLRL
                     LPSPQWESRERIVSSEELFRLFRKASDPVRRLMVMALLTTLRQSKLIETHEEWLVQRA
                     DGWWMMPSPGSRHKRVPKEVPISDLALKALYGDLPRIGGRFFAHWKDGNSFKHRWAEL
                     CRRAGVHDLNFHDLRHTAASWLLEAGIDYAVIEKLLGHRLQGMGEGYIHNWEHRLREA
                     VRKLEVILVQKLSETEQENEKSVGSCGQLGSYEKFEFPKWWILWCRGTESNCRHQPFQ
                     SGGLILWDFGVAPILHDAPIVASATNRGGSRRVAKDPPLCPSHRFAFREGSVAPNMVN
                     VVCHA"
     gene            complement(21976..22461)
                     /locus_tag="PK224_03345"
     CDS             complement(21976..22461)
                     /locus_tag="PK224_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB14803.1"
                     /translation="MEVASQPNKEALIRSWIHRAGQTDALAALCLSFSPNPNAGESLA
                     WLRRAADLGLPDAQLLLGADLEVGFKSPPNSHEAMRWYRKAAQADFPFAQYELGSIYS
                     EGRANQRQDLPEAYYWATLAARHQFQPAKELADTLRPQLTHDERARIDGRIKKWKRVV
                     L"
     gene            complement(22759..25950)
                     /gene="dnaE"
                     /locus_tag="PK224_03350"
     CDS             complement(22759..25950)
                     /gene="dnaE"
                     /locus_tag="PK224_03350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRB14804.1"
                     /translation="MPTPFVHLHTHTTYSLLDGATLLDPLIEHVRWLKQPAVGITDHG
                     NLFGTIPFYTKARAPGIKPIIGCEVYMAKGSLLDKKPPEGPRDFYHLILLAQNSLGYQ
                     NLIRLVSHGYTEGFYYKPRIDKSLLRSHSSGLIALSGCLDGEIPTLLHQEQFASASVA
                     AQDFLDIFGPDRFYLELQANGLHQQQRVNQGLIHVHRELGIPLVATNDCHYLKRGDAD
                     AHDLLLCIQTGTTITDTTRLRFGTDQLYVKSSEEMAEAFRELPQAITHTNLIAEQCTL
                     EIPLHRTQLPRYNVPPPHTLDSFLEILAQQGLSARLAHHSGPLDQPQYERRLRTELLT
                     ICSMGFAGYFLIVWDIMKFARSRRIPVGPGRGSAAGSLVAYALGITELDPLAYDLLFE
                     RFLNPDRVSLPDIDMHFCMKRRQEVINYVTEKYGRDHVAQIITFGTLGAKAAIRDVGC
                     VLDIPYDEVDTIAKLIPSQPNMTIAQALAEEPKLSALSASRPSVGNLLSVAGSLEGLA
                     RHASTHAAGLVISNTPLLETVPLCRTTSDDIVTQFGMGELEQLGLVKFDFLGLKTLTV
                     IDHTISLINQQSPGSRPLTLDDIPLDDATTFALLASGRTTGVFQLESNGMRELLREFQ
                     PDRFDDITAIIALYRPGPLDLIPEFIQQKRRQCPLASNIEALEPILRTTYGVIVYQEQ
                     IMAIASTVAGFSLSQADILRRAMGKKKPEEMRALHTQFVEGAVGLGTERDAAEELFQM
                     ITKFAGYGFNKSHAAAYALLTYRTAYLKAHNPLAYMTALMTSDFGDHEQISLYASECR
                     EMGVRLLPPDINRSETGFTIVDGAMLYGLAAIKHVGEHLATQLIRTRATGGTFASFFD
                     LCDRVDRRIVTKRVLDALIKSGALDTLCDHRAQLSAILDDETPAKPTRSHRNVTQQTL
                     LHFLPVESETRVLPQIPTWAPSIQLDHERETLGCYVSHHPVRVHDHQLKRLQVVPLTQ
                     LPDLPDGKEVRIAGLITRVKRITTKRQQPMAFVHLEDQDRTLEVVIFPSLYRHESGQL
                     EPGTVIALEGILDQNDQGIKVKATRFLPLDPAA"
     gene            complement(27652..28566)
                     /locus_tag="PK224_03355"
     CDS             complement(27652..28566)
                     /locus_tag="PK224_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRB14805.1"
                     /translation="MSSRQVVTAGNRSIEQNTSTHLDLSHVHAVNVLPKQCHRLHLIQ
                     IGCGGIGAFLGIHVARLARECFRLFDTVKVTFYDGDTIEEKNLRRQNFCQAEIGHNKA
                     EALAFRISTAWGIPIRAFPRHFSEKVAQPEYDTLTILLGCVDTARARRSLHQALARLN
                     TRNDAQAWLIDGGNLTTHGQVLVGNVIKDFKPDESFQLRTVCQSLPAPALVHPELLKR
                     ERRPVNTQPRSCAELALSDPQSLTINSMIASHMADYLLRLVITKDLRRFATYIDMDTG
                     SARSLAITSTEISRALGLHQNKSMGRAS"
     gene            complement(29199..29879)
                     /locus_tag="PK224_03360"
     CDS             complement(29199..29879)
                     /locus_tag="PK224_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14806.1"
                     /translation="MRDASSGHDASLHFIGGQLLLEYKDGEAVVRKCISPEAARNAFS
                     SAGIDSDWLPEHVCRYGIGPGGPWLILRFPPGRYLVPLADPIRLSGGGAPHTMLAVPM
                     PGLLFLGYGTRYYVWAYKLWKYAATKLFKAPLANVYPDGAICFGNVHPRVAHGNTIAN
                     SWRLFWDSNFSDHLASGKSTTHPDNILPFLAKLHSTEAQDYPLDDLEPAHLSIAAIIR
                     QLMESGKG"
     gene            complement(29876..30658)
                     /locus_tag="PK224_03365"
     CDS             complement(29876..30658)
                     /locus_tag="PK224_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14807.1"
                     /translation="MNLVLASLKNTEVISVPSCLEYLEERERDFCIVNTFHEIFPRRF
                     RTAAAALPHRSIKEAAEQLQEEFIKLVEQHYFPLDSWDTEWIRDRFPFIPIHLENYDC
                     ESEFEEVPLPICIAAAWVGHYSYAPTWTNIQEELSEHATIPRCFLNPQHRCNVQFLDF
                     RRRCARRRFPISGFPQVIDILGHCTGSLFLDISHDCESPDHPYQWNNRDLKALARQWK
                     RAQLMQEAMNSTTKALLDNPSAWETIFSCWSDLCTATHEAQT"
     gene            complement(30675..31112)
                     /locus_tag="PK224_03370"
     CDS             complement(30675..31112)
                     /locus_tag="PK224_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14808.1"
                     /translation="MVTLQLEGQEIQLPPEIAISDDAVRKAIAPLFPQAAHATIQRTT
                     DTNGMTTISLTKQAGTKGNYHVILRQLIESPRHVNPALTLYQTYQAESQPTQATVESI
                     FLTQQKLRLAIEHGEKEYKETATIKERLTSTPGSPSRILPPGF"
     gene            complement(31126..31659)
                     /locus_tag="PK224_03375"
     CDS             complement(31126..31659)
                     /locus_tag="PK224_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14809.1"
                     /translation="MKTEAVPDPPPPRRDEEDDEDEQDPIVSLEQDSNDFQRATISIV
                     MTLLPTDRHPDGRLTLIGVRSHNLPPQLTTARFNDLLPLPESLSQALTRWEQTFPAAL
                     TARKTQRDARAAEQQTRSLEQEKKREAERHARKAKVAKPTAAKTTSVAPTPKPDREQH
                     SDDSPSNTALPQAGLFE"
     gene            complement(31946..33607)
                     /locus_tag="PK224_03380"
     CDS             complement(31946..33607)
                     /locus_tag="PK224_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14810.1"
                     /translation="MPAGIRTSLTRVYTYPAYLTYSSYAALPAAVREEADQMQSLWNA
                     MVDVYEVAYRQQERLHKQFLQTSRTTTRAAGGTRSIGDPLECIKQLRGQNDSLEDATL
                     LALSRTYRTAAAEYEASQHHNIDRCLPYPESMKQELSQITKRLNDTLHQLARTSPLSR
                     SHSNAVLERMKTAIRRVPLGNLPPSKETDPPTSLFFTHNFGSQGIRIQSLTRADMTQS
                     GSSFAANRLLTLNPASQLAEDTDPRDRGQRDFCRTEGELFVRNVPIPFEIILTPLLPK
                     DALLKQVSLIGSQQRPQASVKLDSSNARLRNDTTWQWHLQFTLIVPPTQQTEPKKYGM
                     IALDFDHRLINDAKPLVRVGEWVSDTGKHESVFFPARILKNLIAAYEAREALNVQHLA
                     LKQLFVHFPFGEALADMVKEPIHSDVPLSRKKLRRIAHQAVEKAYSAPLTSIIREILS
                     QIRDTRIDVVRRRRQWRRDRTEFYDKLAHRLSREAGTIVLTPILMPGPSGSEGSSTCT
                     TRSAPLAQALAHPINMQEFIHRLRHAAPIYRTTLHFVQSMHALIA"
     gene            34116..34358
                     /locus_tag="PK224_03385"
     CDS             34116..34358
                     /locus_tag="PK224_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14811.1"
                     /translation="MRWADQYNVMRSMLLSSPATHWWVRDLILRLDAKDPVDVLNGLE
                     VVARLMRMRHEAVRAGEWDQLECQKPTGDDGISMKD"
     gene            34825..35307
                     /locus_tag="PK224_03390"
     CDS             34825..35307
                     /locus_tag="PK224_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14812.1"
                     /translation="MLNIHRIDHEASHTHCWRVTVQRRTRIYVRSFSDGRYGGAEQAL
                     VAATTYREALIRSNPPLSKPVYCAILKKTNRSGVSGLSRIDRWEFSKGRRVHRLYWDV
                     QWPIENCQSRHKKFSILKYGEDGAYRRALKARAAALKAISSQTFSPFASRGAANNAAA
                     "
     gene            complement(35331..36227)
                     /locus_tag="PK224_03395"
     CDS             complement(35331..36227)
                     /locus_tag="PK224_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRB14813.1"
                     /translation="MPLSPLIRFGTSTWTYEGWQGQIYTRKYTKTAFTRECLGEFCQY
                     LYRGQPLFRTVGNDATFYRPPSANQLTRYLTQIPEDFQMCFKVWEELTIPTYAKHARY
                     GSRAGQVNPNFLNADAFTKLVLQPYRDAQFGPNTGPFLFEFQRHGMPAEEFIGKLDDF
                     FDSVSAEFRYAVEIRNAGLLGPRYHDMLRAHGVAHVYNHWSYMPPLAEQHQRMKTMTA
                     PFTVIRLLTPLKMTYEAAKKRAEPYDKIVGELPEMRKDTVSLIQEATAENRSTYVLVN
                     NRAEGNAPLTIQALIDRLQTER"
     gene            complement(36247..39279)
                     /locus_tag="PK224_03400"
     CDS             complement(36247..39279)
                     /locus_tag="PK224_03400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRB14814.1"
                     /translation="MSPDFVHLHTHSSYSPMWGTSTLEALCEAVRSQGHDTFALTDTN
                     GLYGAIRFLEIAKRAGLKPILGTEIIQAQHRAVLLAKSPEGYGNLCRLLSARHEDEPF
                     EFIETIQRYRAGLVILSDDFQALTAWRAQSGEDLYVELTPGADLQKAVSFSRQNCIPP
                     VATTRAALLRPTDFEAHRLLRAIAENTTLSRLRPDCCCAPSHWLMPSSVMARALPHVP
                     EALSNTRRIANQCATAWDFQTTIFPSFREMSADGAVETLRAKTYNGAVWRYGVLSEVV
                     RARIEKELTVIREKRFADYFLVVDEIVRQAPRTCGRGSAAASIVSYCLGITHVDPIRH
                     HLLFERFLNPGRHDPPDIDIDFPWDERPKILEWVFARYGAHQAAMVANQNTLAARAAM
                     REIAKVYGLPAAEIGKALDLLHRRADFVEVTTDATLQTWAAQVCKALRLNPPWLDILF
                     WAGQLQGHFRHLGLHPGGVVLVPDEIRRYVPVETSASGWPVIQWEKDQAEDAGLVKID
                     LLGNRSLAVIRDALAAIAANGGRTIDYAAWDPISDTATQELIRRGDTMGCFYVESPAT
                     RLLLKKLWTTMPPPQRAQADVFEYLVAVSSIIRPAANVFADDFVRRAHGQPYRALHPL
                     LETVLAETHGIMVYQEDVMKVAMALGGFSMHDGDQLRKILSKKHKERQLRDYQRQFYA
                     GAAARQIARTVVDQVWAMMMSFAGYSFCKPHSASYAQVSFKSAYLRAHYPAEFLAAVI
                     SNQGGYYSAFAYLSEGRRIGLTMLPPDINTSAWAYRGSGRTIRVGLMQLKGLREDSAQ
                     HIVADREAHGPYRSLQHFLDRLQPEPAQTTLLIKAGCFDSVAGELTRPALIWRLFADQ
                     SGKPVGYLPIPAEYSAQRKLAHELELFGFPLNGHPLELFTDVVAGLSPLPARDLAQHI
                     GKEVTLLGWMVTEKVVSTKKGEPMEFVTFEDQTSLYDATLFPDTYRQYCHLLASNQAY
                     LVTGVVEEHFATVTVTVTQLRPLSASDTEETMLLEEDA"
     gene            complement(39280..40464)
                     /locus_tag="PK224_03405"
     CDS             complement(39280..40464)
                     /locus_tag="PK224_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14815.1"
                     /translation="MDRQIVCFAIPSLEIALVRLGHPQLRTRPLAVAKLDTPRALIRE
                     ISPEAERDGLHIGMTLDRARRLCPSLQVRLPNPHQVATATHSIFGVIQRYAPTWEPLL
                     PGSLLMDLTGTTRLFGTACDVATKIQTEVLTQFHLEGAAGVGSNKLVAQTAATLIAPS
                     ELYDVRSGSERLFMSPLSIRALPGVHRPCMRTVLKRLDDLNLCSLGDVADSPLEALEV
                     ALGTYAGQLSRWAQGIDPTPVLRPVAHPSLSNTITLTPDEVDDARLQGRLEDCLQQLC
                     RTLRSQRRMCGGLSLTLRYSDHREVSTKARVMPDTCWEIDLSGLLQGLFQRSFQRRIR
                     LRGMTVTLAELTDYAEQGSLFDARPPEEQRTRDRSQRLALALDRLHQRFGEQAIRYGR
                     AQ"
     gene            complement(40496..41041)
                     /gene="lexA"
                     /locus_tag="PK224_03410"
     CDS             complement(40496..41041)
                     /gene="lexA"
                     /locus_tag="PK224_03410"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRB14816.1"
                     /translation="MTPQEFKRVRLDLGLTQQALADQLKRKRLAVTRYEGGARTIPGV
                     VEVALQAIAQSARIPLVGIVAAGDPIEPIPQTERIEVPKSMLGRGETFALRVKGTSMR
                     DDGILPGDVVVVQKQDMARNGQTVIALLNGEATIKTYHRKAGMIELHPANDTMTPILV
                     HETDTFHIEGIVVGVIRHLKR"
     gene            complement(41359..42921)
                     /locus_tag="PK224_03415"
     CDS             complement(41359..42921)
                     /locus_tag="PK224_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14817.1"
                     /translation="MLYAVKKKDANAYRRWLHWLDAEAANTKICSVQPDGTIGSDCQT
                     IEWPRVCTHDLGYSEEPAPFKIFGKEGGRCALRPPDALDFAAVNDATHTAPPKRMAAW
                     ETESRVTLKAILSLPGVSEIPPLLLVSATDPVHFPLHLDATRVLLRMLINNPSLRTSN
                     LPKLPKDTDLLSFFDPLAATGSDPISISAAAKVISARASWNPFYRLLAEGPTPTVRSM
                     ILESCPTAQDRDQDINKSSWLWEKEKTQFTKANSMYWDCLFLGTLYNRMRVKQSVVSE
                     LLDTFLQYADRTDTILQLANRNLQNAEAALGLAQKTLDEANRAQKDALEYAGNAYAQQ
                     NQRITSELQSLQRQGANAAASITGVQNRIAQQEADIVSMLDTIPQSITKQVCPDRPPF
                     DPVGDALCRNVTTITSIANPAKDAARSALENLRSQHTLLTRTLVDIDKGITQAQSELA
                     ELDARHVKALADVSSKVFDLAIKQATADLVLQTRVTQGLRQAVATARESHARFLDYVS
                     VWRPKNEGSDTH"
     gene            43619..43897
                     /locus_tag="PK224_03420"
     CDS             43619..43897
                     /locus_tag="PK224_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB14818.1"
                     /translation="MRLITTKELRALHIATRHHHLINGRLAIFRYADEYGFNGAARQF
                     GLDRKTVRTWHRRWVVSGPARLVPRQGIFGWRIHRAMSSTGMLVPAVG"
     gene            44898..45206
                     /locus_tag="PK224_03425"
     CDS             44898..45206
                     /locus_tag="PK224_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14819.1"
                     /translation="MRTLPRLAAGLSVLLLTGCVDLSFRFTPENLWVKPMAEGKTCIY
                     TLFNLGYGTNTVEQAMANATPPIQKIRSISGTMFHFIVFESHCLKVVGESAPAGEGTR
                     "
     gene            45243..45545
                     /locus_tag="PK224_03430"
     CDS             45243..45545
                     /locus_tag="PK224_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14820.1"
                     /translation="MRTFCTLTACLAVLLLMGCVSITNRITPEDLSVKPMAEGKTCMY
                     TLFELGYGTNTVEQAMANATPPIQKIRSVSTSLFFLLFVNKSCLIVVGEGAPAAAP"
     gene            45604..45906
                     /locus_tag="PK224_03435"
     CDS             45604..45906
                     /locus_tag="PK224_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14821.1"
                     /translation="MHRLICCTCWLTALLLSGCINVSNRLTPEDLSVKPMAVGKDCMY
                     AAFGFGYGSTTVEQAMANATPPIQNIRSVGVSLFYLLGVARECLIVVGEGAPGTTK"
     gene            complement(46710..47825)
                     /locus_tag="PK224_03440"
     CDS             complement(46710..47825)
                     /locus_tag="PK224_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HRB14822.1"
                     /translation="MIILFALLYVIVTTSEVGAWGEEGHRGIAEAVQGHLTASTAKSI
                     AKIVGTGNDLPPSTLARLSVWPDQIRALTKNPHTTIPGFSPAEMEEAKQFVATHPDNT
                     NWHFVDLPPGVAHYPDLAHPDPADPALPFTNTTDVVHMIHQSVDILEGRTDSATFTKL
                     QALRWLLHLSEDIHQPLHVASGYYSTAADTLSHPTMLTDPSEVTKQHGKNDRGGNVLL
                     FLADPTCPTKPTHENLHAIWDDCLVDVVGGAKGCVSQTTDKTVARVADHLKARMEDPA
                     SQAFRMRGDYHRWPEQWATDSVHVAAAGIFPAELDSGCVILDSKRPHPPVHVQSRVVT
                     PPTKSAYLQEHKVDADIQLTKAAVRLADLLNHLQWKE"
     gene            complement(48130..48417)
                     /locus_tag="PK224_03445"
     CDS             complement(48130..48417)
                     /locus_tag="PK224_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRB14823.1"
                     /translation="MAKAMTKDGIAEYLAEKSHLTKKAATQILDDLAALAYKESKNGF
                     KLPGLGMLVLKNRKARMGRNPQTGDAIKIPAKRVVKFRIAKAAKDAILGKK"
     gene            complement(48616..49782)
                     /locus_tag="PK224_03450"
     CDS             complement(48616..49782)
                     /locus_tag="PK224_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HRB14824.1"
                     /translation="MKLNIQAFVLFLTLMVGAALLQGPIAVEANDSTTTHVTETSERG
                     DTIAPNDSCVQSITTDLVQKLKPISSVAIAPDEPSDAANSAVLRYLNDMTAKIAASPA
                     LTRRSYDGKDKQVVISFRVHARGELSDVRLERTSSPTNKIFDPYDLSAKGAVEQAAPF
                     SAFPSDISTRWLDATATISYPSRESLVEEQHAKVRAYSICKDLAHIGLSIYTMKDNAA
                     DLEKLQALADQLQKYKQELGDEFEVSRLQGNLTDKTTEIKKQIARISKLNKFDNDCEN
                     KLHKQHFPSHLRDEPVNSSHPDTSTLGRAFCTAINGGGTVEFLVMNERSPTVGIAVIT
                     RKRRFVVILQKKMTGDLTEEWRAVEVQTPSDRKAIPFLLGNSFMASFPEMVQDE"
     gene            50287..51234
                     /locus_tag="PK224_03455"
     CDS             50287..51234
                     /locus_tag="PK224_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14825.1"
                     /translation="MKKYIAVFDETGTSNRPQSTEESGFGVGAVLFPLEQAPELARIS
                     KHLGSIVGKEDYKYKDVLQNEAARELFIQTLNRTSLSIHLFAFYAHGACMIHEGRRTK
                     DAALAYGREYKERSSPRGERFVPFDSFIAYMASCIAAHAATNEYAVDIYWDRRTDLDQ
                     IKASFDEHIARQSDTQRYRNVAELVSFKGRAQNDLALITRLAGVLAGDVWRYFESHGP
                     KIWTALDVTGLPGEYDPHMTIDDPDVTGPPCVAVFMERLIKVEPVRTSATSMLQGYYK
                     RFLKNENKENLISFGDPHGRLGIIGIVNGHHWKVYQLPD"
     gene            51335..52294
                     /locus_tag="PK224_03460"
     CDS             51335..52294
                     /locus_tag="PK224_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB14826.1"
                     /translation="MKVRRTSEKGNSTSRGNDAGLWGGPAWTSTVESVVRSCVSPHSK
                     RAYSASLRAYRMWLQQTPQPEFSRLSILRYRDALLHRKHLAPKSINLQLTALRKLAKE
                     AVDEGLIDDRTAAGILRIPMVPNRGVRAGVWLPREQAQRILSGPNVRTIVGKRDYLVL
                     MLILRCGLRRAEIAQVAVTVVREHEGRWVLANLRGKGKRLRTLPLPGVVKNAWDRWVE
                     ATGIESGRVIRAINRHGRVWGEGVTEDTIWRIVGRYAQLALGKHVAPHDLRRTFGRLC
                     YALSKDMREIQQLYGHSSVMTTEKYLGVEPHLVDSVTDRLGLS"
     gene            complement(52882..53103)
                     /locus_tag="PK224_03465"
     CDS             complement(52882..53103)
                     /locus_tag="PK224_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14827.1"
                     /translation="MSDEVTADFLLLKQILMFAINTGGGIRHGGLIEDDNYDRLIALE
                     GKIPLPGDREVVGVFRRTGRTLSDTNEAT"
     gene            54177..56996
                     /locus_tag="PK224_03470"
     CDS             54177..56996
                     /locus_tag="PK224_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRB14828.1"
                     /translation="MVCKSNAFNGTLLKNKKWMNFSENVTFSAEEYFEPQHTSPGGPP
                     DGLQQLVHVVARATNEQKALHAIGSGWAYESLAQSDSWVVNLKNLNRRLHNVIPTALT
                     DGWKEKQGNAAATTHLVHVEAGMKIIDLIEMLEGVGLAMPTLGGANGQNLAGAISTST
                     HGGDWNQPPLPDVVRGIHLVTDGGRELWIERASEPITTDVRLKPVLTCPDTEIIRNDS
                     IFDAALVSFGRIGVIYAFVLEVRKEFRVVEVATTADDDELGELLLALMAGMTSPNDPF
                     EKLFSRLRAAPLPPGLAETVELARPENKPYFFQFVWSSAAPSSLNPFHAPQDTTYVQR
                     RWITTVGTNLNPPIDDFMADMTKGLLSVIPDGLATVSGQAFVASGGLKHLATKALEER
                     FKKAVTEGLRASHHLLTSGTKRASEDIPYSGDYIEVIFDARDDRIIPFLGTIRENRSA
                     HDTLGWISLRPSRASRASLSMHNVATSYAISIEVALLKGPSGNRGFMDLVHEAALKYG
                     GRPHWGQKNNLNFQQVVSLYGSQLQNWRTSLTQVSASSTLFSNNFTRQRGLEPTNTER
                     KVTALSASKNELIVVGLRKGNDKYNHEVQFSHWASPAVAELQPLSPRHPVQFDHGRRS
                     PSQEDRGWVGWHPLEPASGLPDNFIGSAVEGGKAYVFWIASDGWVNYRVRGQDGHWAR
                     YWLPVGNTNARPWNGVPGGAVHAVSCQPGTLHVFYTDRQGGILAVRGNTTSAPGWPHS
                     QWIKGCKTIAGGHVTAVSRSQGLVDAFVVGTDGRVYTAAWNAQDNWSNWRVVKEGLIA
                     KPGSYISAVSRNVNLLDIFVADEGGKTLSAAWAPPSGWNGWWHIQGGISRPGGYVTGI
                     SRSSDKLDIFTTDPDQRILTAAWDPGVGWAGWWPIGNAKAQSLVWPVSRSQDKIDLFF
                     VDPDGSVQTSAWAPGHPWDGPWSIS"
     gene            complement(57169..58407)
                     /locus_tag="PK224_03475"
     CDS             complement(57169..58407)
                     /locus_tag="PK224_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB14829.1"
                     /translation="MTSITALAIFWISLSLAYTQILSSSPVAFADEINLSDELSSQET
                     NPCNLWLSGTSRSSTSASLTPLGDTNAINGEQPGGLREYLSQIQTQISRFWSLLPRNL
                     PEAVLTTVVNFRLDRAGMITNVNIQQSSGNEYSDLAARQALKSAVPLPQFPRYIADRY
                     LDVVYTFSVENLNATSDRSRGPVVRRRGLAGTELVVINQSASEESWGRQKYQYSACRT
                     HLRTLPQIIDEVPNNQTGLKKLQGVANGLRFPDINLESFDDSDVPGLIKDIRHRIAAR
                     SEEITLKLATDSEMDRFDHVCKSEILKAGFPYDLREEPVASSRPETKTLGRAFCTAIN
                     GGGKVEFKIIDKNPPMVGIAVTTKKRQFVVVLQKKMQGDLSEEWTAIEIQTPSDRKPI
                     INMLGYSFMDIRFPALVQNE"
     gene            complement(58424..59032)
                     /locus_tag="PK224_03480"
     CDS             complement(58424..59032)
                     /locus_tag="PK224_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14830.1"
                     /translation="MDISTNEHCPPSCSQLIGKQLPWSAIGKEWLNSTLAFVRKRMSK
                     CMHSICGAVLVLTQVIGCSLLGPVVDGYSCGNAAARSDLQKITLKELVKNGWPEGTTP
                     LIQTRVTYPEDVSRNNPDYKCRALLHAKVGERGYESFTFAVHYNYDDYKVTDYKSDFT
                     KYQRMKIADMLSGKMEPLAQKAKVCTQTAKAGGCEDGRDIFH"
     gene            complement(59128..59748)
                     /locus_tag="PK224_03485"
     CDS             complement(59128..59748)
                     /locus_tag="PK224_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14831.1"
                     /translation="MAERWDGLRIALWLVLVWASIFPHPIWAQNQESNSASVPFGEAD
                     INGGLRDAFLAKLAEMRSITKRKDAAMAMYGELARVRRDFKNHLHKLDLQIESVESKG
                     PSSTLSERQHLLHLRALARQIQHHINYNGIHMDSLLAEYDVLSRVAELESSYKKDEIT
                     KADFAMESRALRGELAQVNAERRVNSLGENIMEGFAHIESQMMSMH"
     gene            complement(60046..61202)
                     /locus_tag="PK224_03490"
     CDS             complement(join(60046..60929,60929..61202))
                     /locus_tag="PK224_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612037.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB14832.1"
                     /translation="MAGVTRRRYTEDFKQEAVRLVRDSARPVAQVARDLGIAESLLYR
                     WRTQHRQAEAQGTTRAIQRTDGEELTYLKRELVRVTQERDFLRRAAAFLREGIAMRYR
                     AIQEYDRRYSIRLMCRALNVSPAGYYAWRDRPESRRARQNRTLLATIRVIHRASRETY
                     GSPSIWNALSQQGHDVGKHRVARLMRQAGLRAKTVKKWRATTQSNHRLPVAENTLNRQ
                     FAVAQPNRVWAGDITYVWTDEGWLYLAVLLDLYSRAVIGWAMGYRLTLDLAEHALTMA
                     LANRTPTAGLLHHSDRGSQYAATRYQQLLTAHGITPSMSRRGNCWDNACVESFFGTLK
                     RELVYHQHYATRDEARREIFEYIEVFYNRKRRHSALGYHSPAEFEARMAVA"
     gene            62428..63060
                     /locus_tag="PK224_03495"
     CDS             62428..63060
                     /locus_tag="PK224_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14833.1"
                     /translation="MTKKIVQPALSKELSFYLDHASEADDLYENKGILSKAKLDPRFE
                     TAFENVYEKAYKDLRHLITHLSNHVSARVKRYACIEWKHRNVEKEWGCWGYARSKGRR
                     AAHHWTGIYLDHSKGLARMVLILSPSGGRGGRRLFVDRFRPIWRKKRERLVLTEDHPK
                     DWPEWEETPCIVYYVHELHPRSSLNETCSATEKVVEQFFRLAMPKLNKKN"
     gene            63199..64325
                     /locus_tag="PK224_03500"
     CDS             join(63199..63454,63454..64325)
                     /locus_tag="PK224_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB14834.1"
                     /translation="MKRTRRNHGATFKAQVALAAVKGDKTLAELAEHFQVHPTQITEW
                     KQQLLARAADVFGGTTPTADTPDLKTLHAKIGQLALENDFLGRGAHQGGLAERKAMID
                     RTHLLPVRRQCQLLKLARSTAYYHATPVSETALALMRRIDELHLAHPFAGARMLRDLL
                     RQEGQTIGRRHVATLMRRMGIEALYRKPSLSRRHPAHQVYPYLLRDLTISRPNQVWAA
                     DITYIPMARGFVYLFAVLDWASRRVLAWRLSNTLTTDFCLDAVQDAVGRYGPPEIFNT
                     DQGCQFTSQEFTGLLTHHGIQISMDGKGCWRDNVFVERLWRSIKYEEVYLHAYETIGA
                     AHQGLAQYLTFYNQTRPHQALDGQTPDQVYSDNLTTRLTAA"
     gene            65246..66730
                     /locus_tag="PK224_03505"
     CDS             65246..66730
                     /locus_tag="PK224_03505"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HRB14835.1"
                     /translation="MPIVTNNGQPFFYTKGKIMNVVFLVLGIFLLFGCVPKDMLAKNE
                     GERPILCKEFKATEADGLSDTYVANRSFWRTFNAHYSRNDSGSNSNGPRKLLVFMDGT
                     GNDKSSSTNVRKLYRLAVQHACSGKRIIPYYDKGVGAKLINRVTGGIGGRGTSLNIRQ
                     AYRFLIETYLPGDEIYIIGFSRGAFTARSLNGLIEFAGLVDRRSIPPKWYDTFEWIGA
                     TNLHFSIGALYDVYHQDFDGTAEFEERIRGELKTLMDSRGMTAYKDKDKVKVTAIGVF
                     DTVPALGLIRDDEPDDHRLELYADNGFHALALDEQRDDFRLLRFDPLRAVQGSRLEEV
                     WFAGVHSDVGGSYSLEYNCTSKDNFNGLATTPLNWMLNKFSKFDIFPPTDELIKLADQ
                     EKNGFLQCGCAHEANPFIECNCGLLHDEFFDGGFGFFQNMGTFPRRPHPGDNIHRSVL
                     QRIRSAKIFKPHTRREAGGRYLFLGRTISGNSDEFFAKNFKVVE"
     gene            66788..67321
                     /locus_tag="PK224_03510"
     CDS             66788..67321
                     /locus_tag="PK224_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14836.1"
                     /translation="MRQFLIASAVWVMAGELAWSGNDYLNPAAIVSPQKEVLCSQVDD
                     HRLAIGASCVASIKADISENAVGLHLQGGNEYIVSVDGEQAWYDASHKHIPPQGDSGT
                     LLMNLFALLKKNREADWFALIAEIKGTNKCHLHDLSKSSNLLAEEDGSLVLYANDARG
                     FYWNNHGEITVIISRKK"
     gene            67505..69631
                     /locus_tag="PK224_03515"
     CDS             67505..69631
                     /locus_tag="PK224_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14837.1"
                     /translation="MGSKGYFLIVALTTYFVGSAVAASFAGPRIEWEVGNRYRAFDYK
                     AGVKQSAEQSAKQFEGYRQEAGENVEGWFKRIIEQQHSPYIADPGPWNESTGKYEKDF
                     VNVPKLVFVKAHLVTDKDRTEVLTQKPCIWRISTNETSQPCNDPFVIAGFDSGGAKVS
                     VLQDGVEIASAEIKPDFKIILGLGDSYAAGEGNPDSPTKWEENAKRKWPPVNDTATGD
                     RVLKTAQWWSNRCDRSFYSYQSLAALHVASANPHAVVAFVHLACSGAEIIDGLLAPQR
                     FPPGHSMHRCQPPFERSDPNKWDKQCDVPYSQLNAAVDLLCPEASKPLESDKINKIKS
                     ALKGIWHNKAQVNWLNEQPKERLRFCQKPLRKIDYVLISIGGNDVGFSGLIADALLPR
                     DTHIRTPLLGKLAKQIVQIVQKEGKVVCPYLPAGTSPEDLKRQKQCREYGLSAQTRIH
                     DIPQRYRALKLALEEILQMDPHEKTLTPDKIILNLYPNPLYKTGGALCGNPGGSNDEN
                     EWRAIHTVLPPSKSLFGLLTLYNWEVNFTADEARDVEGHVINPLNRALKEIGQEFGWQ
                     VISTESAMKDRGWCTGNSRCLSHVNPTEWSPYTSRSRFIRTANDSLLTQWPRTTMESE
                     SKDSKIIERENGFYGMFHPNAQGHAAIAKVLVDQTQIASSINTEPMPELLLSSSGDKL
                     LTLTQRDSVADEDATCDSKVIVKKNK"
     gene            complement(69856..70536)
                     /locus_tag="PK224_03520"
     CDS             complement(69856..70536)
                     /locus_tag="PK224_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14838.1"
                     /translation="MGESLGFQVALSSRVIMTIFCLACLWGCSDPQAKDRARLCEGAG
                     VAVDLVKECRRSWEDYNRIMKPIAERLEHEEITAFNLALRALPPRAIPKARYESTSLA
                     ELSKALEPIEMSEVSNWTKHPIFGRRFRVNAVLQFHATDFESRLSEHTSLGQRDANDA
                     SQIEVDTESLSREERAFAKTQCDYVLQECHGEVFGVVGVVTRDQIPHIGLQLEHMEIK
                     PRALKKSS"
     gene            71630..71848
                     /locus_tag="PK224_03525"
     CDS             71630..71848
                     /locus_tag="PK224_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14839.1"
                     /translation="MKKDYAVSVIFPEVLVKHSRQHIRISANNLRLAANEALKAIMKR
                     EGIKGRRLTRLQLAVIHAGTSTIQDHEA"
     gene            71976..72350
                     /locus_tag="PK224_03530"
     CDS             71976..72350
                     /locus_tag="PK224_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14840.1"
                     /translation="MATADNDSFGVGMTIIADFFANQICRLLEPKQLGYTFTSLILLN
                     ATTIILLEMSIGWTATFYSLGPIGFGLLDILNKPSRFGPLYPQLWGSPGPSTSTEPWR
                     PADLGHGRTLMSVCRRQTPPGG"
     gene            72453..73886
                     /gene="xseA"
                     /locus_tag="PK224_03535"
     CDS             72453..73886
                     /gene="xseA"
                     /locus_tag="PK224_03535"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRB14841.1"
                     /translation="MTMSLPSTHSTETPQPILSIGMLSQRIRRQLAPMLTKQTYLLQA
                     QWIKPQANKGQKGFFSVTLADTDNPNMAIDAMIWEPAAIRKVLEQGQQFGHDLLSRDS
                     RVEFVVEATLGFWANKNTIYVLIHQLSTIGMLGLRQQQREAALRTLKEEGLLTRNASL
                     PWPRFPLRIGIIGKQGSDAVHDILNVLHSSPYRFETTIFHTAVQGVGAIPGLLKAFHD
                     VAAHTTPLDVIVMSRGGGNEIDLVAFDNLDVARAVATASKPVLTGLGHHLDRSICDEV
                     AAKALSTPTAAGQYLVSHLDRIRECLFTTHATLHAHTRHTLLEWTHALSLTRHALWER
                     ARTTIEAHATASHHAHERFQRALRDRLEASYQQGRTLRYSLTASLTQALSQLSMGYAF
                     TLVRLHTAGLSRVDTLKGAITAFLQALSLSDPQHLLARGYAYVLDNHGRMVTSRRTVI
                     AGEQLHIHVQDGRITATTLHTEPSHEQ"
     gene            73876..74112
                     /locus_tag="PK224_03540"
     CDS             73876..74112
                     /locus_tag="PK224_03540"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRB14842.1"
                     /translation="MSNENTSHPQLPTSYDAAFKELEDLSARATKGEINIDELSTAIQ
                     RGNQLFAFLSSGIAKIKSEIEDHAPDGITQSKPS"
     gene            74173..74406
                     /locus_tag="PK224_03545"
     CDS             74173..74406
                     /locus_tag="PK224_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14843.1"
                     /translation="MSKHHHTHPITIDQTLDLLAKQWTAADRIRLRTARHRPRHVRAA
                     ELWTRIQSLTQSTLALFQHPSAMETSHGHHSDR"
     gene            74384..74761
                     /locus_tag="PK224_03550"
     CDS             74384..74761
                     /locus_tag="PK224_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB14844.1"
                     /translation="MAIILIVDDYSEIRACLRTMLEEAGHIIVEAGSGQEALHAWNET
                     HPDLVITDHSMPGMSGVELIRALGQRQPEVKGILMSADRDPRPAEPPDQSHAWPFLQK
                     PFGFRALRQAIDSLLLAQPAEPA"
     gene            74872..75801
                     /locus_tag="PK224_03555"
     CDS             74872..75801
                     /locus_tag="PK224_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14845.1"
                     /translation="MVDSDFLSYAQLCQFVREQGRLPRLDDPIPAYRYAGWALPMIWE
                     GHRVLPRVPNRWGYHLDILHARRIPDAPIPQIEFLHTPHRDTLTHLQRWVTLAAEHQS
                     SWTGLTNLIAWIAFSLKLNHVPPSLADNTQAALYQAVNLQELVLHPYDYWGDIIAEGI
                     GRGPWANPNGFYPTPMSVCQLIVRMMLPDADSLSPAARNALRVKSIADPAGSGTGRML
                     LLASNVSLSLYGIERDALVRTACLINGALYAPWLAFPLPQEILHGTDADHGLTNQASL
                     LSVATPIPEEISLIPPINDAVDHEDLQLVFPGW"
     gene            75906..76349
                     /locus_tag="PK224_03560"
     CDS             75906..76349
                     /locus_tag="PK224_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14846.1"
                     /translation="MRVLEAHGDLWDVHAQGSWIAITTNGVIRTNGHGVMGAGLAKQA
                     ADRFPSLPSLLGTHLRRFGNIPRSIPSMRIMTLPTKHHFRTASDLALIQNSLQHIQLI
                     LTRERIDRLYCPHPGCGLGQLSWQQVRAAIVHVVDHRFLFLHSTA"
     gene            77528..78028
                     /gene="radC"
                     /locus_tag="PK224_03565"
     CDS             77528..78028
                     /gene="radC"
                     /locus_tag="PK224_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRB14847.1"
                     /translation="MKTALDQHQLGIYDVPRYTISLIKEAASTFHAKTRCQSSEDVYR
                     YFKDICENQDREHLYALFLDTKHVPIGMNHVAMGTLSTTIVHPREVFKCAILLNAACL
                     VIVHNHPSGDPSPSPEDRALTTRIAEAGQLLGIALLDHVVIGNGRYFSFADHGPLTQQ
                     APTYTS"
     gene            78803..79063
                     /locus_tag="PK224_03570"
     CDS             78803..79063
                     /locus_tag="PK224_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14848.1"
                     /translation="MTPSIRCPFRRRSIPRHHPFWPPNSNQIDSGIRTIYDLSKGIAL
                     FGVFVPLVTFNGVWYKLVLAVSAGLAFFLTAWYLEGRNGSTL"
     gene            79047..79184
                     /locus_tag="PK224_03575"
     CDS             79047..79184
                     /locus_tag="PK224_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14849.1"
                     /translation="MDLHCDLDTAVLIGFNFIGWSLFLGVLLKDRLTHFQRTQTSQAT
                     R"
     gene            79862..80218
                     /locus_tag="PK224_03580"
     CDS             79862..80218
                     /locus_tag="PK224_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRB14850.1"
                     /translation="MSGKRDLNFSLYSGRLTHPPEMMQYNTGHGHMLCFSLAIHSDAP
                     SDKIDNPMFLDVKVTGASPELHQHMSVGQEVRVRGELQSRTFKKQDRIQRTYYLHVKH
                     PSDLEFLSRPKDKTTT"
     gene            80286..81710
                     /locus_tag="PK224_03585"
     CDS             80286..81710
                     /locus_tag="PK224_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14851.1"
                     /translation="MTTAQITHAQIARALYEAHSSVECLSIEDLRELQLSTLGSNANS
                     ETSWLPALPDRVLVTTEAEEEEAITLIDHLFQAHMDAWSLKDRDQVFSRGTRFDDENP
                     APQYPLITKSSRRTSDPSFMVLPDGRRVLQLDAQRLLTLCGIQEVLLREEVRPGAFAR
                     AGRGKCPSCHGLHLATPQSAEATPARASGNDLVCPRCKVSIQSNYIQEDADTLKCLCC
                     SHRIYREHAPELSGLDAEDIAQNEERVARACYVDHGQQAEADDDALDLDSGQDSEDLF
                     DDTTQIDTFEYLDYESSHEIAQDDIPNDESDVDLPDLQDKLHAPAYALFCQGRHPLRQ
                     ALLNQAILELDAHDNRMPVWVAAGRILTSTPHHLSPTDAQAITSWLHGLPQEDLAILA
                     GDPNASNERLAHPEPGLFFPTNQLLLPLIRFFGHSLKRFRNKEAGCRILAADYGLTES
                     ALKHIAMAYGQLPANREASHVPAA"
     gene            81773..82105
                     /locus_tag="PK224_03590"
     CDS             81773..82105
                     /locus_tag="PK224_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRB14852.1"
                     /translation="MTYAHVELVGRVATTPVRATVGARRVTNLRVAIDRPIQPTRPGH
                     TPPLFLSVCIWDHTLDHQLPSLTKGQTVKVTGRLDTRTSSHTGLTYTHVVTDHIELMP
                     PEIHRQAA"
     gene            complement(82263..82514)
                     /locus_tag="PK224_03595"
     CDS             complement(82263..82514)
                     /locus_tag="PK224_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14853.1"
                     /translation="MNVIDTIINEIVQHIHGSSCIPETYEAAFVTAVWERAQALEAPL
                     RDELLCRTGVTQDSVKQIREEAADALYEAHRALYGTAWE"
     gene            complement(82610..83851)
                     /locus_tag="PK224_03600"
     CDS             complement(82610..83851)
                     /locus_tag="PK224_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14854.1"
                     /translation="MTTSHLTSKHATLRGLLSAAPRRTPVVDATWVRSEMAKAIVSVV
                     VQDFQRIAADHGRLVHAWERLALEDLQRLVWELEKPRYQVHPAQWARLRARLADLTDI
                     ERRNAKMLNARTPFENRYDDDDDRGETWEDMAELDYTVPAPRGTRAAQAIDATVDTPL
                     CPDCGSSSVVVSMMTVGVHQLDLKEAHCLICAWRVELGRKPSATVDVESDLELEDHGD
                     GTLAGPIDESEESADDDEQDIVPSSMAGEEETQRSVVEDFETQDMESAEDPVDQLMTL
                     QNRFWQDWTVKPLRCALLEGTMRERLSGERLDMIWAVASTLMESCRGLGRAEQQEIAR
                     WLMRTSTEELRAFLPESELPAELAQPERMLVVPSVSTKIQKWQLACRLALRRFRVASA
                     GVRILAPSFGVSPDVFRAASA"
     gene            84011..84250
                     /locus_tag="PK224_03605"
     CDS             84011..84250
                     /locus_tag="PK224_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14855.1"
                     /translation="MRLTPELEITRQLSTFVNSRQELLIANARFVGSREFCALEGFSE
                     RVLSQVNHLTQALSLTEQEQALARDITDAVAALSR"
     gene            complement(84219..84467)
                     /locus_tag="PK224_03610"
     CDS             complement(84219..84467)
                     /locus_tag="PK224_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14856.1"
                     /translation="MYTNQAVSVDMRIVGKLVLVDLHGSGDALQLAFNDLANRFELLI
                     EAGALKWSKARADHFVLMPQSDWTSLEVISETKRQLRL"
     gene            85603..88074
                     /locus_tag="PK224_03615"
     CDS             85603..88074
                     /locus_tag="PK224_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRB14857.1"
                     /translation="MPQAPVALHASRSGKPASTRALLLIEQFQTALQDELQSRTPRQR
                     QETICVPLTDGQRQHVTGRTHYYLFQSEPIPANLVEDTNPSLVLISQRIPCRIVGFSP
                     DGLALAFEGEAPETIPSAHLTFDSVRLLQALDKRLQSISQQPDTFGTALALKAFDPDA
                     IATITALDRLPEASGLNPEQAQAYTSALERDLLFVLGPPGTGKTAFITALSQALLVQN
                     GRTLICSPTNTAIDNILEHLLSQSPDLAIDQVIRIGTPSPDSSDQCQMANLETVALTH
                     TLPLEERAKTLRRQLQDLDRDMPYLAHSADSAKRLDTHWRELQTLRERHQMLHTDERR
                     LRGLITERAMTIQELEGELQAFNASPAFRRLFEHGNKARIVNDLACIRDYQAADEITL
                     RSLQSQVLHSQVRIDTLNRVMEQFRQDHQLPDAPWSPEQLCTLHEAMQGIRHDLQGTL
                     DRLENEIAQIEQALVRRSRIVATTLTRTYTSRLLDDQRFDTVIIDEASMALAPALFCT
                     LALATSRVIIVGDFLQLAPIASAKTQATKHWLARSIYDIAQITSGRDPRVVPLSMQYR
                     MHPDIANVSRRLYARAGLSYVSAPSTLTDRSQTAEAAPAPGHSLIFCNTEPAHPVTVR
                     DAKGSPFNLYHALLAVRLAQQALSTPGHLPTVSIICPYRAQVNLIERLLRLEELDELV
                     RVGTIHRFQGRESDVVIFDTVTTENATRSMLGWAYDDAAPHKLINVALTRAKGKLIVI
                     GHHGALAPLQHGPQPILWECIEEANANGLHLDATTILQAMTFSLHDSPIESDTVLSRV
                     LARTTASRGQLKPVPSRLTSLVQAS"
     gene            88199..88690
                     /locus_tag="PK224_03620"
     CDS             88199..88690
                     /locus_tag="PK224_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14858.1"
                     /translation="MDTSPTPRPLTYDEKRASEAAFLGLPAHPSWTQGAQRIYVKLTQ
                     ALAKKQPPTDLLNEDLCPRLSSLPAVNETTNLTPDRCQVWHLSLHAPAEQHLFVLDPQ
                     HPINDVVQTIETRWPGRAYQLDPVTCGVFALLNIPQEAFNQHPERQIIHSLIAPARPS
                     SST"
     gene            88845..89228
                     /locus_tag="PK224_03625"
     CDS             88845..89228
                     /locus_tag="PK224_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRB14859.1"
                     /translation="MNTSTQTPQEMKKPYRDTNKIEIAGQLGQNPEIVALTSGRIIVT
                     GSLASHRNYVKDGQTAKKTNWVPFTYRGDKPEEFANALRKGDQVLLLGTLDYREWIDE
                     STQTKRSTLEVAAFEYKLIREGKHH"
     gene            complement(89693..89884)
                     /locus_tag="PK224_03630"
     CDS             complement(89693..89884)
                     /locus_tag="PK224_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14860.1"
                     /translation="MPIGTVDRGEVSEFEGEWVALIVPSSFVVQDPAEERVADTDSDE
                     EPADDNRKDRSVLHSGLQS"
     gene            89972..90319
                     /locus_tag="PK224_03635"
     CDS             89972..90319
                     /locus_tag="PK224_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14861.1"
                     /translation="MSKPAAAKRYTFPSPMSVGIALTALIRAYGYHCHSPEPYSAHSC
                     TYLVENPHDAADTGGYTITRKTASAEEYLLTIHRATAPHMRTLIERARNEAAAQSSIQ
                     IVSSRRAKTQAAA"
     gene            90425..90835
                     /locus_tag="PK224_03640"
     CDS             90425..90835
                     /locus_tag="PK224_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14862.1"
                     /translation="MRSTRPLLIELLCFLLLVVTNMSWAQSQSITPIPEEPSDTQLVD
                     EDVNIATGFYVRDYAVGGSRTVNYRTARQIVNVLHNEFENTVVETLPAPLFYWFDRDG
                     TGRFSMWIEAQGTGCVCDVRPYADSSLTVAPRVP"
     gene            90904..91722
                     /locus_tag="PK224_03645"
     CDS             90904..91722
                     /locus_tag="PK224_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14863.1"
                     /translation="MIPHHTQAQRASEQARRLADTFIGRLTQSGYTATVGAPVNYGHK
                     VSLAHPSLPHTLHAVLYVGKDKTSFVKEGKNWPDGLYDVLLQEFHTLLLPHPMPLVAP
                     ITQAAGSTVAYVDGSYCEQDHNAHIGWAFEIWREGQSIDGQAGSISHPDALSLRNVAG
                     ECHAVEQVLEWCRAHDCTDIEIRFDYTGLAHWANGTWRTNAVRTQRYRERVASSRVRI
                     TWTKIQAHNGEYGNARVDFFARHAATNHVFFPELPLSILPPRLRHSQVTPAPGQ"
     gene            91863..92261
                     /locus_tag="PK224_03650"
     CDS             91863..92261
                     /locus_tag="PK224_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14864.1"
                     /translation="MRMLALVMLILVLGVHSNLVKAEEITPNTLPTIEPTLIEMPFVE
                     GQGASYPVSDQARACRLALPKAQLALAKSHAHMLRTLAVRPTSDPIIDITNHYDRYTR
                     ICTVTIRLTWPTVYTYPPVKLATDYRVPTP"
     gene            92323..92685
                     /locus_tag="PK224_03655"
     CDS             92323..92685
                     /locus_tag="PK224_03655"
                     /inference="COORDINATES: protein motif:HMM:NF022151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HRB14865.1"
                     /translation="MKRVVLVSGSRDFPSKELVMDVMRKSFRAGDCLIHGCARGVDSW
                     AEEAARLAGVPVSRRPADWDRYGKSAGMLRNSAMLHETLEAPSYLILIFWDGRSRGTK
                     HMIDACQVRGATYSVVHS"
     gene            92734..93144
                     /locus_tag="PK224_03660"
     CDS             92734..93144
                     /locus_tag="PK224_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14866.1"
                     /translation="MGRTTPLCPQCQEIGPDTVIEHSTLGLIQYETCRDCTFHNLSRT
                     SSQGQFVMSCYATQDELNRDRYALHYLTCVRDMKYQMYQISTAYPRYTLTRRGQSGRT
                     YDNAHYEFWCKVEALIEHLFTPCSDGPPQLSSFP"
     gene            93226..93414
                     /locus_tag="PK224_03665"
     CDS             93226..93414
                     /locus_tag="PK224_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14867.1"
                     /translation="MRHTGFHMPISETLKLRTLRHVTEQQSVDTLARLRAQDREMDKK
                     DVGAIARAIRAGQSRITL"
BASE COUNT        21032 a        25210 c        25585 g        21652 t
ORIGIN      
        1 ggtgaagcca ggctaccact taccacactg tccgaatttc cggagccccc tctcatttcg
       61 accatctggt ctcgcggtca atgaactact cccttctaca ttttctatgt caatgtctgg
      121 tcatagacag tttccaaatg attccctccc gttgaccccg ccaagtctat taggcagcga
      181 cacacgtata aaccttggct ttgttcagcc attgggtaaa gtcggaggtg ttacgggcca
      241 actcacgtcg ctaacacttg ctccagtgcc catccccgca gctgtgtggc tgtttggaag
      301 tggtttggct gcagtgaccg cgctggccag acgcaagcga aaagcgacgc ttgaatccac
      361 cggctcctaa ttcgtccgtt aaaggagctg cccctggaca tggatctcat ctgcatgtcc
      421 cgggctatcc actccatcaa atccttcact cttcacctgc cactagtacg gctcttccta
      481 ccgttcattc ttaatcactt actgtagatt caaaggcagg agtggactgc gcaagataaa
      541 tttccctgtt gataccactg gttgcatggg ggacgatggc atggcagaat cgagcggaca
      601 cctggagaag caatgagacg cttagcgtac acgttcctgg cgttttgtgt gctggttcca
      661 tgctcatctc tctacgcttg ggatactagt ggacatgcga ttgtggcgat gctggcggag
      721 gagcgcttaa ctccgacggc caaagctgcg gtgtctgacc tgttgcaagg gcaccctctc
      781 gtcgatgtcg cgtcttgggc ggaccaggtc cgcaaccagc agaccgctcc gtggcattat
      841 gtaaatatcg agattgacga aacccaatac gatacgcatc ggcattgccc agaccgacaa
      901 tgcgtcacag agcaggttga acggtttcga gccacgctcg ccgacgattc gctcgatcca
      961 atgaagcgtc aaaaggcgct gaagtacctc gtgcactttg tagccgatct ccatcaacca
     1021 cttcatgccg gacagaacca tgaccgaggc ggaaacgatg cgaaggtgga attcctgggg
     1081 cgggcggtga atcccttcac caaaaagccg tggaaccttc atcaagtgtg ggacaatgga
     1141 ttgctggatc agtatgcgcc taatgcaaag caggccgtca tacagattga tcagtatctg
     1201 gcatcgcaga atgaagccga gttggcgatc ggctcggtca tccagtgggc aatgcagtcc
     1261 cacgatcacg ctagagatca tgcctacagg ttcggcacag acaagcgtct taatgagcaa
     1321 tacgtcaaac aggccctccc ggttattgag gcccaacttg cgagagcagg ggtcaggttg
     1381 gcattcctgc tcaatacggc gttgaggact gaagcggtcg attcagtagt aggggaaggg
     1441 gtctcaagtc ctatacagta aggagcgcag aaatgtccga ttcattgacc ccccgcgaga
     1501 ccttcacaac cgaagagggc gtccttgcta acatgtgggc cattgaggcc ttggttgaat
     1561 tgcttgaacg gaagggattc tgtacgaagc aggaagtgca cgacttaatt gcagagctac
     1621 gcgtacgtca tgctgaatta aacggcaaag tcatcgtgcc gggacctcca gccaacacca
     1681 aagaggagca gtatcttatt gatcatgtgc tcaaagtcat tgaggcggta ggtttacagc
     1741 cagcggagtc gaaagagctc cttcgccggg tctcgttaat cattgaccag caagcacgca
     1801 cgaagctgaa gccgaattaa acacgattcc gatgggatct ccctccaatc cacaagacgg
     1861 ccaaccgttc ccagtgccgt acctgtgcac cgaagtctcg gatcgactga tccaacagat
     1921 tctggatgcc ttcaatcgtg aaggcctgac tgcagagcag gcccgacaac tcttgggccg
     1981 ggtcaagcac ttgctcgacg ccggagagct cctcgcacgc cgcacctctc attagccgcc
     2041 ccatttacct cttctcgtcg atcccacgga gaggaggttt ctcggggaat gccgttcctc
     2101 tttcgtctat cttgtcgcct gatcggacct gtcgtcgtct cggcccatga acacacccac
     2161 gacagatgaa ctcattgccg aaggagatca atgaattcat cctcagctgt gacaatgtct
     2221 acatcatttt cttaaatatc gttagaagct tttggcaata ggtgggatcg gcggagaact
     2281 cggcgccggt tagcggccga ttcaagaact cattataccc gggcagcgct cggacctcgg
     2341 gagagttcag atcacgaaat cccatggacc aattggggaa ttgtcgggcc agaatcgttg
     2401 tctgcaagag cgtcagtatt ccggtgtggc gctcatcctc gaagattttt gcatacagat
     2461 tcagcaccgc ttgttcctcg ccctcaagcg cctgcataaa gtttccgttt ttgtagagca
     2521 atattcccgt gatgcctaat tggctgttgt tcgcatggca agaggcgagc aggtctgtta
     2581 gttgagacgg agaaaacggt cttgtcgctg aactggcata cacgaggcaa aacatgatcc
     2641 cctccctacg gtaatgtggc gatggtcagt cgtggcaata tgccgcgcgg atgtgccaag
     2701 ggaagcccgt gggtctttag attattttcc cgccatgcgt gcgcttcacc aaattaattt
     2761 agtttttctt tctatccgag gtatccccag ttcgtgacac tgttccttgt ccgtttgccc
     2821 tctggtgtga tctttccaca attgagaagt cttgcggcct cagtccgaga caacggtaag
     2881 atcctacatc cgacagacct cccactcgta caggctttcc caccctctgt ggaatgtccg
     2941 tgtccgcagt gcctcgcgta gagtcgcaac atgcacacat cgaggtcctc tcacggtgtc
     3001 tcagatttcc caatctcctg caccctctgt ttggtgcaag aaagcgtgat tggaaccggc
     3061 tggctgtggg agctctctcc cgcacggggc ctcgtccaaa gcgcgctcct ggctagcccc
     3121 ggcatggtcg tgaaggtatc cttgcaggtg cctggtacga cactgattcg cttggaaggt
     3181 ctcgtcacat gggctcgcgt ttcggagttt gggatagcgt ggctctacgg ccatgcctcc
     3241 ttgactgaga aaagggttat ctatgcggca cctcggtagc gcgttcttgc tcggcgtgct
     3301 cttggcgctg gcgctagtgc tggtatcttg ggggcgcccc cctcaatcgg gggcgatacc
     3361 aggcaaaagc cccttcactc gtaccctcaa tcagttggat ttgatcgacg accagcctac
     3421 gcactgactg ccggtctctg gtttctgctg ccagcctctt ccttcgtggt acgccctctg
     3481 agtaatgcca ggtcacgtct agaccgctat ccttctgtca tgcattaccg cggtccagtc
     3541 agccgaacgt gcgagtctgt ccaaccacaa ggaggccctg tgcacccacg ctataatgat
     3601 cggaagccag cgtcagcgaa ggccactctg tcagccggac ctcgagtggg cgaggggcag
     3661 gtgctggatc tcacgaatcc cgggtgtctc ttgcacacag cggttccgtt ggaacgagac
     3721 caggctgtgc aattggtcat ctatctcgac gtgcttcggc ccatgcgcat cacactcggc
     3781 atcgtccgat gggtgtccgg aaagaccgcc ggtattgaat tcattcggat gtcctcagaa
     3841 gaccaagcac gactccgcac attcatcggt cataaacccc agttgcgggt gagcggccga
     3901 tggggagaaa cacctctgtg tgtggcctac tgacatgtca cagctggaga gccgagaggc
     3961 ggagcgacga gtcaagggtg tgctgttctc tagcatggtt ggggtgctgg tagggctcat
     4021 ggtggcaacg ttcatggagt cagagcatcg gtcaccgtcg cctgaccatc cagctccagt
     4081 tctgaatgat cagccgaacg aacctaacga catgagcaac gggaacaagt ctcgagaata
     4141 agggccccat gcttaatatt cgccgagttg atcacgacgc gtcgtccacg cactgctggc
     4201 gggtcaaggt ccagcgtcgg aaacagcttt tcacgcgcga cttcagcgac gggcttcacg
     4261 gaggtcgaga agcagcgctc caagccgccc aggcgtaccg tgacatgctg gtccggacac
     4321 aggcgccttt gagcaagcca gactattgcg cgattctgaa aaagaataat cgttcaggag
     4381 tgtcaggtct gacgcgtgtc gaccgttggg aattgttacg ggggcgacgt gtgcgaaaag
     4441 tcttctgggg agtgcaatgg ccgattggca acggacgatc ccgtcacaaa acgttttcca
     4501 ttatgaaata cggagaggag gcagcttttc agctggcatt ggccgcgcgc gaaacggcgc
     4561 tccaggccct gatttcgcaa acgttttctc cattcgcgtg ccgagacgga aagtcctagc
     4621 ttcccaaccc tttcatcatt tgccggcgaa cctgatcggc actgagaagc agctctcaga
     4681 gtatcctttt gcagcggctg ttaacttcgt tttcctcaga tgaaatgagc tggattgagg
     4741 ggagctttgc tctggagtga acacgtcccg aacccgtagg gataatattt agcatctagc
     4801 aaattgatga tcgtggcgtt atttcctata ggaatcccag gaaatttcac atgaccagtc
     4861 ggcctatttg gttaggccgg acaatcccgc tacgatgaac gaaccgagaa tccatccggc
     4921 caccttctga aaatagaaat agtagcgaac ccatccgacg agatcgatgg agtagtgctg
     4981 ttcccttagt ttgatgatcg gtagaagcat gtcaaagctg aagaaaatga ggtctagtcc
     5041 atatatcaaa tatccgaagg ggtagcgagc gaagggaatc tgcttggcaa ccccttgcct
     5101 cgagatccat agaattgtcg cacccactaa tattaagccg atggcccaaa aaaaagagag
     5161 atatatgcga tagccgtatc caatcaacac ccattcaagc cacaagctca ctttctgcaa
     5221 accctctgct acatagtact cccgattccg actctcaaat aggacgtcat tcgctttctc
     5281 aacatatccc gatgttcgta atacatttgc aaggtgttgg tatgactgcc tcgagtaggg
     5341 caattgaccc aggagccact ttcccatcgc atcagcccct atcaattcgg gagcattgcc
     5401 tttggcaata tcagtgtttg cggtgccgct aattctagag taagtaaaac cagttagtcc
     5461 taccttgctc ggtatcaccg cctgcgcctt ttcgttgtca tctgggtagt agatcctgaa
     5521 tgcccccgtg tcagtattga tcatggatat cctgccattt ttgttccaat gcagggctga
     5581 tttgaacgag ctgccagcaa gtacaaattc gcctcgtatg cttgtctcgc tcaaacttag
     5641 agaggtgagc gagctagcaa ttaggtttaa gtctcctcca atgctactat ttgaaatttt
     5701 cattgaggtt cctgttatgc cagtaacgga aacccctcca ttagtttgca gcccggtcat
     5761 ctcgacatgg cctttatacg ctgactcgtc tagctccaat cctccctgta cttttgtaga
     5821 gcgaattatg actctgttaa attttccgcc gccacgaagg aaaatatttc cattcacctc
     5881 tagagcccta atatccaaga cgccggaaac ttcagcgcca tccaactcaa catttctttc
     5941 aattttgctg cctccgagca gctctatatc ggtgtatcgt gagttgcggc caatgattgc
     6001 gccggctcga atgcctcgca tgtccaatgg tttcatgaaa gtggcgtcta ccatctttag
     6061 cccgattcgg atgttggcgt tacttatgtc aacatcacca gagaagtggc taccgcttag
     6121 actgacggtg ctgtcagtat gtatttgctc catgtcaaga tcatcattga attttgagtc
     6181 ttcaaacgag atgagttgag acgagcggag atttgtgaaa attaccgttg actcaaattt
     6241 gcaattttcg agtcggatag gatttgcaag gactgcatgg ctcagatcga atatctcctt
     6301 aaaccatgct cctcgaatcg taactccgta atgagttatt gcggaactgt acggctggtg
     6361 cagaatgatt gtttcgagaa agcgtggtga aacgactcgg aaggagttat ctacgtgctc
     6421 attgttgttc cactcaccta aatcggcatc gtcaccgata cagatcttcg cccagataga
     6481 tttttctgtc gatgaccagt tatcactcgc taatagaggc gtagtgcacg cgccatttgg
     6541 tttagcgggt tgcccgtgtc gatctactaa tttttcgtga gaggcaaaga tctcaggact
     6601 catgatggtc agtgttaata aagccaacga tgtcgcaatc agaagttgat tgagcccgaa
     6661 tgatttgctc atgtgagaat tcgattaaaa tggtattgga ttgcagtcac ttttgttcct
     6721 gatagcaagg atgcacgatt ggctgtacgg atatcttcct aaactaatat gtaatccggc
     6781 aagtcgactc ttggtccaaa agcacagttc tagcatcgtg ttgtaagctc ccccggcaag
     6841 gcgacactgc caacggagaa attcgggctt cacgttgggc tctatacgtg gcgggcggga
     6901 gccccggcac gtatcgtgcg gccgctcttc gttctaacgg cgtaaccatt cgacgctgat
     6961 cacccggacc tgagccaagg actcgaatac atacgccttt cagtaaacct ggtctggcgc
     7021 atgggaatct tctgtctgag ggtgtctact gtgccagaaa gttctcctta atgagtgctg
     7081 tgatttgcgg ggagcttaca cgccagatct acagaatctc agtccctgtt gtatcattgc
     7141 cactcacatc ctcagcaaga ttgggggaaa taccaccaga taaattcatc tgcgcttcaa
     7201 tacatccaag tatttctgct agcttccacc aatcctcctt gggaagacga atcttattgc
     7261 cggtaaggtg tgatgaaaag cgtgagaaga atgatgaggc agattgaggt gatccctgta
     7321 ttccttccaa gatcttgatg acatctgtat gccagcggga ctgtgctcta acggcgttca
     7381 aaatctcctc taacatgttc tctgttgttc gtggcggcgc tggaatatcc tcgctatatt
     7441 gtgttacccg gtgcaggtct tcttcgaact ttggccacca catagcaaat gatgaatcta
     7501 ggctctgctc tgaaaggcta tgtttgccca tcattttatt tatagattga agcatcttta
     7561 gagtttcgtg tttgttcgca attgttgcat ggaattgcgc gagtgggaat gtaaggtctg
     7621 aaggggatag cccgagtagg tatgggcaca cgcgggagtg ttccagctgc tttgataggg
     7681 agccagcttc aaagttgatc caaggcttat caatattatc aggtgtaaga caaatgatac
     7741 cgatctgtgc ctgctctagc tgccctgaga tttcagtggc ccatcgactg cccttgtcta
     7801 aatcactgtc tgacatccat ggctctaggt tctggatgac ttgtggcagc cagtccctta
     7861 aagcttgaga cagttgcttg ctctttcttc ccgaccaagt taggaacacc tttgttttcg
     7921 gcatggatca ctcttttgag tcttaaagtg cccggactag atgtggcgct atcgagtatg
     7981 cttcagccga gtagccttca ttaaggacct gctatcctct agtttgggca tgcctctttg
     8041 agcgtgtagg gtacgattat gggcatcgca tcgtgccatt cctccggatg tgaatttatg
     8101 tgttcttcaa ccatgaattg cccgactgtc agaccttctc ggcaatattg cagcagcgac
     8161 gcttattccg aattagtcat cacgtcgtga tacgcctgtg cgaatcgcct gctttctaaa
     8221 gtagttgcta cgtacatcgt ctttggcatc tgtggtagcc ctctctgttc attccctgtg
     8281 atactccgtg tcacaggtgg aaagaatcga gtcagcgtga aaacagcgag acgtgaacat
     8341 tgcatcttca ttgtgttgcc ttgttgccaa aaccatagcg atggctttca gcaatggaca
     8401 ggtcttgata caacttcatc gttgttaggg cttaacgata actctgaatg gaagttggta
     8461 tgtctccgtc aaatagtttt cggtcacact aaggaggcgg agctggggct cgctaattac
     8521 ggcttttgac actccaggtt gtaccacgaa gatcttcatg gtgattcgag ttgtatggct
     8581 catctccctg agcatctcta tcaaccggaa atctccccgt tcgattctag aagatgcgcc
     8641 acgagcctgt cgttgggatt ctcgacgtaa taagtgagtg aagagatccg agcgcttttc
     8701 gccagatgac atccaccgaa tgctcgtctg tgcttgacca cagacgacgt acaggtcgtc
     8761 gatacgcccg cctgacttcg ccttcttcga gtatttgcag tgataaagtt cgacgtcgat
     8821 ttgttccgga gcatcaaatg agccgatgac cgttgcggca acgacatcgg cggcttcacc
     8881 cgatccatcg tcatcaaaga tcaaatggta ggagttacct ttgaggcgct tgatcacggc
     8941 cgcctgaacg gagtcttgat ctttggcatc accttgagat tctttctgaa tgttgatgcc
     9001 cgtccaatcc caattatcaa cgagcttctc aactgaataa ggagggaaga cggttttcag
     9061 tggagtgtac tcgttcccgt caagcgacgc gccgtcagca aaccaaacac gaggtgggtt
     9121 gctagtaaaa aagtcttccg ccggaatcgc tgtttcagct tttataatat ggaccgctga
     9181 gtcgctgagc ggcttgaagc ggaagtcgga cgtctcgtcc aatcgataaa tctcaagctc
     9241 gaactcaacg tgagcatgcc cgctggtgat cccgaacttg attggcccag ataatgttgg
     9301 attgaccaat tcaaggtcca cttcgctgat gtggaacatc gtactgtcca tcgagattga
     9361 ccatggcgtt tcaatgctct tatagatctc ttccggccag tctatgacaa ccggtactcc
     9421 ctcgggacga gtagccacaa ccttcgtttc cagcgtaccg cttagcacag catctgggtc
     9481 aatcgtcgag tcgatcactt ttcctcctat atgcttgcac cactcgacga gctgctgtat
     9541 gcgatcacgc cggtgcgacc agattcgacc tttacgcgat gctcctactg tggctgtagc
     9601 tcccccttca aagccggttc ccgagagatc cgactttctc gtcctacgtc tctgggcgtc
     9661 ggcgagaacc gtcgcgacat ctgatcccat tcggcttgta tagctaacgt ttcgtccgag
     9721 ttgctcggct aagccaacgc tcttcaagcg cagtcgcatt actccgtgga atgaacgaaa
     9781 gacgacttgg cccttgatca tgcggacgtc atccccggcg aaggcttgag ccaggctctt
     9841 gaattcccca gcgtttgtcg agccgttgat gaatagaagc ttcttctctg gccaccaaat
     9901 gatgacgtac aactcccatt cccagctgaa aaccttcact atgtcggtcc acggcagtgg
     9961 tactcgtctt gccgttacga taaccgacgt gtgctctttt tcgttggtag cgacatgtac
    10021 ctgttcgcag gaagcaatgt ttgaaattcc agactgaatg ttgtcggtcc tccaattatc
    10081 acattgcgtt ctgtatatga ctgtgctgag cgccgggcgt atggtcttta gcggaatctc
    10141 cttggcgaaa tcgctgaagc ctctcaagaa ttcctggagc gactgctgct cgccaatcat
    10201 ggcgtcgcta agctgtggca atagtacatt ccaatcagga tcgcgtgagt agagctttcg
    10261 cagctcatct tgaacggaga ggtcagcagt gttcgcaaca aatgtcgcat ctcccaggtc
    10321 tggcctcgat cgagtgaagc gtccagcgag ttgaagcgtt accgaaagcg ttttcctgat
    10381 atcatgaaat gcggcgatct tcagttccgg tagatcgaag ccctcgccga gcatatcgac
    10441 acagacgaca atcctggatg tctttgtcag aatcttgcgt cgagcttcct ctcgtgctcg
    10501 aatgcttttt atgcctgtat gcaattgaac aatttcaaga tccggtgcaa ggcgtttata
    10561 aatctctgcc acttgaatcg cgcgtgacac agtctcaacg cgtgccataa ggatgtggcc
    10621 tttactgtaa tcggtacgca gctgttcaat cgctttcttc gcgattgcgt tatcggactg
    10681 tttcgggttg aactccacaa ctttcaggaa cctgattggt ttgaagtagc cttcggcttg
    10741 agccttcttc aaggggtata cgtaaataat gtcaccgtcg agcggtttgc catcttctcg
    10801 aaacggtgtc gctgtaaact gtagaacccg cttcccagag aatcggttct tgaaggtgtt
    10861 ccaagtaggg gctttggtat ggtgtgcctc gtcgatgaag agatggctgc aatatttagc
    10921 cattgcctgt tggacgttcg gtgggcagga gccaacgatg ctgctggtcg taatgattac
    10981 atgtgcaggt gaaaacagta acttgacatc gtcggacgtt ttgggaatgt gttgcagcac
    11041 acatacaatc gggtgaagcg cactaggagc aaggatattg gcatcttgaa gttttaatac
    11101 gcctaaggtg agaaattttt ttgctagctg agttcgaagg gcatctgtcg ggacgattac
    11161 aagtacccgc tcacaacatg cagagacaag aatcgagagc atcgtctcag tttttccggt
    11221 accggtcggc atgaccacag tcgccggagc ttccgtgaca gaccaatgtg cgtggacggc
    11281 atgcaccgct ccgatttgtg gcggacgcaa ccctattaca ctgctgtcgg ttttcgctac
    11341 cacatacgtg aaagcactct tccacgaatc gagtactttc ctgtgttcga cggcgtgatc
    11401 tcggtgttga ccttctacga tcattggatg gcgaagccac ttgccttcta ttaggtccgc
    11461 tacactcgta ctgccttcac taaagtgtgc tgcaatgatt cttacgtgct ccttaggcac
    11521 tttctcgata ttggtctttg gcagaacgat gaacagcgat tcgttgccgt gagtgggctg
    11581 aaacagccag ccacgaacat tgtttgtggt aaatggtgtg agtgttccct cggattctct
    11641 gctagggtca cctatttgtt gaaggtcgtt ttttaaaggc gattcatcga gggtaagagc
    11701 gtaaggggtt ttgagtatca ttgcgtctaa tactagaaat cgctcgggct atataggttc
    11761 agtggccaac ggctccgcat ggtaccgcca catatcctct ataggcaaca caccggtatc
    11821 cttccgttac cgcactgaaa gtagtcgcgc atctctattc tgtcacgtcg caatcctgcc
    11881 ttttcttcct aaaacccaca ctgtaatgtt tcggcttcgt ctccttgata gaagtgagag
    11941 agccatttgg tcgaggatgg tactcattcc aattccttcc ctaaaccatg aaggcgcatg
    12001 atgaaaaata gggccgtttg gatctgccgg tagttttttg gccctggagc aactttttgc
    12061 tgtcttgtgc cgtaattttg atcgcggcat catcaccatt cgccttacca gggttccgtt
    12121 cgccctatta cggcttcatt actacttgga cacaaacatt tgtatctcag ccaccagcgc
    12181 ggtgttttct catctcttac ctccgccgtt gctcgtcgta caaccttttt gcatagccgt
    12241 cacccgtcgg ttgtgatgcg ctgaaaagcg cgttagccac gttgacgtag tgtcccttag
    12301 tgtcccctcc tgtcccccag tgtccccgaa ccggcctctt tcactaccgc acgccgatgc
    12361 gtatggttcg gccatacatc gtgtgcagca acaaaaggag acgtgatgga acacacctgg
    12421 ttgactgtcg atgagttggc cgccgtcctc aaagtgagcc ctaagtcgat ccgccgtgcc
    12481 taccgcaagg gcgagattcc cgtggatcga ttctgccggt ttgtgcggtt cgacctggag
    12541 cgcgtgaagg aagcgctgaa ggcgaagggc catgatctca ccgttgtcga ggccgggaag
    12601 aacgtgggac agcgcagcgc gaccggcggc gaaagccggc ggcgcgcgca gcggcccagc
    12661 ccccgacttg gtaagacggg ggcgtctatc gctcagacgc caaggaggaa gaaatgactg
    12721 tttctggagg attcactcag accatcgatt ggctcgcctt cacgctgccg aaagctgagg
    12781 tggcagatgt gatcacgctg attggtggcg actggttcca gagtgagagc ggctttcgcg
    12841 gctatcccgt ggcgcagctc atgacgcagg ggaagaccgg tgtcggcaaa ttggggacgg
    12901 gtgctcctcg caatccaaaa gaagtgcatg tcgatctgtc cgccgggatt gtctcccagt
    12961 gggacgaaac caagttgaag acggtgttgg cctggatctt tgcgcagaaa ggccatgtca
    13021 cccgcattga cgtggcgctg gacgatcggg aggcgtctgt cgcagtggag accgtccgtc
    13081 ttgctgtgga ggctggacag gtggtcagcc ggtccaagca gttcaaagtc atccaagcct
    13141 cgaatcatcg ggaaggtgtc cggaccggcg agacgctcta cttcgggagt cgagagagtc
    13201 aaaccatgct gcgcgtctac gataaacgtc tcgaactcca atccagaggc cgagaagacg
    13261 cggccttata cggagtgcga tgggaactgg agttcaaaca ggatcgagcc caggcctgtg
    13321 cgaaagcgct actcacccta gatccggaag attggcgggc gtttttggtt ggcgtgctgc
    13381 gttcctatgt cgatttccga gagacgacac gtgaggccga atcctacgag aaatatcgag
    13441 cgccgttggt gggctggtgg aaagcgctca cggaaggctt catgcgatgc cgactcgtgg
    13501 tcgaacggat tcagcagcgg ttggatgatg tggccgcctg gctggccaac gccatcagtc
    13561 ccatgctggc cgtggtggtc gcctgtcgcg gggatcaatt cctgatggag atgatctatg
    13621 cgggcaccaa gcgatggacg cagaagcact atgccctgct gaagcaacga aggagaggaa
    13681 cgccctatgt gcttcatctc aaacctcgga cctaacgtcg tgtacctccg gacctgtcca
    13741 ggtccagatt gccacgccgc cggagcctgt ggggagtttc gaaaagggaa gttgtttgtg
    13801 atgtgcgagg cgtgtggctt cgaatatgaa ttgctgccaa aagtccggct tgcgggccgg
    13861 ggcgtgtggg tcaggcgcag agctgatccg agagtctttt cacaacaagg aggggtgaac
    13921 gatgcaagtc aaagcagagg gagcggtgca ggggtatgtc gagcggagga gccgggaggg
    13981 caaagtgtac agatcggtgg atttctatgt gaagggcaaa gacccgggtg tgctgcggtt
    14041 gggcattccg gacgatcaga tgtcgctgat cgagatctgt aagcaggcag aaggcaagca
    14101 ggccaaggcg tcgatcgaag tgcggaagtt cgaacagacc ggacgtacat ttttcgacct
    14161 caccggctta gacgtcgtga agtaacggca agagggagga aggccagtgg atctcaccat
    14221 tgttctcgtg gcggtcttgt tactggcctt cctcaccggc ctcggagtcg gacgattgtg
    14281 aaactcttta ctcttactag ttatctctcg ctcatctggg gcctcttgat ctccttctgt
    14341 ctggcacccg ccgaatctct ggcgcaaacc gccacgcagt attcgcgggt ggtcgcgcaa
    14401 gccgaacgca ttgcgtattt ggcggcgcaa cggtcggcct tggcgaatca ggtggccacc
    14461 gctgcactcg cgccctcggc tgcctcgatg gcggttcgca tggtggcagg cccggtggga
    14521 tgggcggcct tgggagtcag tgcgggtttg gtcctggccc agatgtatta ctctcagtct
    14581 gatctttcag cggtgaagac cgccgcctca acgccgggcg gctggcaggt cacaagctcg
    14641 aacgcaggcg tgcagacgtt ccccggtttc gggacgaata cgcccggtaa tccggcttat
    14701 cccaatgcga ccatacagtt cagcgcgacc aacgttccgc tctgtgcggt ggataccgag
    14761 tatcagcatg attgggtggt ggggccgttt cagagtctga gtacggcggt cttctttacc
    14821 gggaactttt tcgtcaatgg tccggcggtc ggcggctaca gtctgtatgt ctgccatcgc
    14881 aagggcattc cggggtccac ctcaccgatt caagagggct tgattccacc gacgcagcag
    14941 caagtggcca acttcgtcgg gggtcttccg gccagcgatc cgaagtctgt cgaagcccat
    15001 accaatcccg tgggcacgaa tggctcgacc cagccagccg ataccacgat ctcccaagcc
    15061 gtgacgccga ccgagatgcc gacgacggtc aaaccgaagc cggtccctgc cggcgacatc
    15121 gtcgtggtcg atacggttcc gcctccggcg agtacgccgc aacagaacac gcaacagcaa
    15181 acgacgacta cgaccacgac gacgacacag aatcccgacg gctccacgac acagcacgaa
    15241 gagacccagg cgacgacctc gtgtgccgtg ggatctcacg agagccggac cttcgggaca
    15301 gtcttgcagg tccatcaaac tatctgggcg accagcgggt tattgggcac gctgaatctc
    15361 ttgaagtcgc tcacctggcc ttcgaccttg ccggtgattg ccttgccgtc ggccttcttt
    15421 ggcagccagc aagtggactt caatcaatgg gcctggttct tcactgtgct ccggtcatta
    15481 gtcattgcga cagcctccat tgccgcctac cgcatcatct tcgtgggtgg aggccagacg
    15541 acatgaccgc cattctcacg ctcatttatt gctggctgca ggaattcttc ttctcgctga
    15601 ccgattgggg attgagcatc tgggattccc tgctctccgt ggcggacagc acgctcgcca
    15661 ccatcgggac ggcagggctc accctgccgg tcattcccga tcaatatgcg tgggtgctgg
    15721 gcgcgacggg catgagtcag gcgctggcca tcgtggcgag cgccatgggc acgcggttta
    15781 ttttgcaaac tattcccttc gtgcggtggg gctcatgaat cagctcgtga tcggctgggc
    15841 ctatgcatgg acggtcatcc tcgcgctgtg gatctgggtg tatctcagga gtcttcgatg
    15901 atcgaactgt atgaaggggt gccgggctca gggaagtcgt atcacgcgat ctgcgagaag
    15961 tttctgccct gggtgaaaca aggccgacgg ctctatatcg ccgtcgatgg gatttatttg
    16021 gaccgattgt cactgttcac gggtatcgag ctgtctgctc tcgaacagca gatcacgatc
    16081 tggaaggact cggtcgaagt cctccaggcc tttccgcatg tcgaaccagg ttctgccgtc
    16141 atcattgacg aagcgcaaac cgtgtttcgg tccatgcaaa aggtcgaacc ggggctccta
    16201 cgctggctcg aaacgcatcg gcactatggc gtggatattc tgctcatgag ccaggatttt
    16261 cggcagatgt cccagggcgt gacgcgactg atcgaagcga cggtgaagtt tcggaagctc
    16321 agctttgtcg ggctctcgaa gaagtatcag ggcaaggtgc gcggcaatcc ggaagatcac
    16381 gaggtcatcc ggaccttcgt cggcacgtat tcgccggccg gctacgccta ctactccagt
    16441 tatgcgtcgg cggcgattcg agaagaaaag cgcagccata ccgtgttcaa gtcggcacgg
    16501 gtggcgatcg gcattgccgc aggcctgttt gccattggtc tcatggtctg gcggccctgg
    16561 tcttcgttga gcgcgagcaa gccaaccact gccgctggat cagcggttct ccctaccacc
    16621 atcggcgcac cagcatccgg ttcggccagt ctgttgaacc ccttcggcat gtctccgcca
    16681 gccccagcaa ctccgcctgc accaaagcgc cccgtgagaa ttctcggtgg cgctgggatg
    16741 agtaaggatc gtcaaggctg gcgctatctc ttggacagcg gcgagatcct gacggccgcg
    16801 cagatcaccg gacggtacgg tctgtcggtc tcggaagtct atgaagacgg ttcgatgcga
    16861 ctcattggca aaggagtgtt ctatggacct gccggcgatt gagacgatga cgtacttcac
    16921 cgcgatcttc tggctcggcg gctttgccgt ggggctgatc atcaaactga ttctgcctca
    16981 gagttactga ggcgttcgac cgtcggcgaa caatccggcg gcgtgggtgg acggaaccac
    17041 taacagcaca aggaggtgcc gtatgaaggg tcggagttgg gtgaaagggc tcggtggcgt
    17101 gctcatggcg ttcgtgttgg ccttgggctt gccggcgctg tcacaggcgc agttgtttcc
    17161 ggtctcggcg gatgtggcca cggtgcgggc cgatctgctc ttgtgggcca cggccttgat
    17221 tggcgtggca ttggcgatct acgcctttaa gcgcgtccgc gcgattgtgg gctgaggaat
    17281 agcccagcga ggcaggctgg cagcagcctg cctccttttt catccggaga atggaagagg
    17341 ctgtagcgat gacaacaagg aggcagcatg acggcgcaac agattcaaac cattggcaat
    17401 gccttggctg tcgatctggt cgcctggggg acggcggcga tcgggctggc gctcgtagcc
    17461 gccggggcag cttgggtgtt gcggctcttg cgctaagcgc cgggccagcc ctggtccgca
    17521 aagctgtagt agcgctcttc agccagaatc agatgatcga ggagggtgat gccgaacagt
    17581 tcaccggcct cgcggagtcg cttggtcaga acccggtctt cgggactcgg cgtgatgtcg
    17641 ccggacggat gattatgcga gcaaagccag gcgccggcat tcatgaggat caacggctta
    17701 aagacttcgc gaggatgcac aaccgtcaag ttgagagagc cagtcgagac gacattgatt
    17761 ccgatgagct tgtgcttggc gtccaggcca cagatgagga attgttcccg gtccagaccg
    17821 gcaaacaacg gccggaggat cgcagcagca ccttcggacg tatgtaccga ttccgccgcc
    17881 ggcatggcac ggccctcacg cacgagcgtg acacggtacc gagggaccgc gtatttccga
    17941 accgggctca gtggccatcc accgttcgag gaaccgatag aactgtcaac ggacatgtcc
    18001 gcctccttct gaaatgatgt tctagcccca cccttcacca atcaagggtg gggcgtaacg
    18061 aaggaggcgg cacggggctc tcgcagcggg caggcctggt ggggcggaga ccgacacggt
    18121 gggaaccggg aaggcctgtc ccgctgctcc ccggccacgg gtcgcacccg cagggatcta
    18181 ttcttcggtc aatgctcgtg gatacgctca atctttactg tgagatgttc tcagaatgcc
    18241 tctagtttgg ccgtggtctt gtgtttgctg ggattcattg agtaccgtat gaccatttta
    18301 accataactg aagcggctaa gtttttacgt ctcagccggc gcacaatgta cacgttgaag
    18361 gaaatcccac gtgttcggta tgcaggaaag cttgtatatg tcctggaaga tttggaagcg
    18421 tgggtccata gccgaagaga gggagcggga atagcggatt taagcggtgt gaagaccaag
    18481 atgatcgacg ctgttctacc tcgaagccgt cgccgcagaa gtatttttgt attatcaaca
    18541 gagattaaat cgaaatgaat attaaacggg ctcaaactcc acgggggacc tgctccattc
    18601 ttgactatcg gtatggtggc atccgctatc gtcccgtgct gggttacgat ctcactacgg
    18661 aggaggaaca agcagctgca attgagatta tggtcgccat tcaagtgaat gtgggcattc
    18721 aagctcctca agcccagcct gcgcggccat cgctctcatt ttgtgaattt gctccgcgct
    18781 atatggccta tctcgaagca aaaaagctga tggctttgga ccggccgctc acgatcctga
    18841 atctgcatct agtcccacat tttggatcag tgccgcttaa gaagttaacg atggatcatg
    18901 ggctgactta cctcgctaag cgtcgtgctg aaggagccgc agaagggacc atttcgcgag
    18961 agtgtggagt cttacaagcc gcactgaact atgccgttga aattgatgca ctggataaaa
    19021 acagattacg ccttttgccg agtccgcaat gggaatcgcg agaacggatc gtgagtagtg
    19081 aggaactctt ccgacttttc cgtaaggcat cagatccggt tcgccggctg atggtgatgg
    19141 cactcctgac cacattacgc cagtcaaagc tgatagaaac tcatgaagag tggctcgtgc
    19201 aacgtgcaga tggatggtgg atgatgccgt ctccaggtag tcggcacaag cgtgttccca
    19261 aggaagtacc tatttcggat ttggccctaa aagcgctgta tggggatctc ccgagaatcg
    19321 ggggtcggtt ctttgcacac tggaaagacg gcaactcctt caagcaccga tgggccgaat
    19381 tatgccggag agccggggtc cacgatctga atttccacga tctcagacat accgcagcat
    19441 cttggttgct tgaagcgggg attgactatg ctgtcatcga gaagttgtta ggtcatcgat
    19501 tgcaaggaat gggggaaggg tacattcata attgggaaca tcgattgcgg gaagcggttc
    19561 gaaaacttga ggtaattttg gtgcagaaat tgtcggaaac tgagcaagaa aatgagaaga
    19621 gcgtgggcag ttgtggacag ttgggcagtt acgaaaaatt tgaattccct aagtggtgga
    19681 ttttatggtg ccgagggaca gaatcgaact gtcgacacca gccttttcag agcggtggtc
    19741 ttatactgtg ggacttcggt gtcgccccaa tactccatga tgccccgatt gtcgcgtcgg
    19801 cgacgaatcg tggcggatct cgcagggtcg ccaaggaccc acccttgtgc ccctctcatc
    19861 gcttcgcatt tcgtgagggt agtgtcgctc ccaatatggt gaatgtcgtc tgccatgcgt
    19921 gagaaatagt ctactcccca tggataggat gaaatgtaca gccgtccgtc cagggctcga
    19981 aatcgttcag aggaagctag ctgagtttcg tcccgaatca tgtgtgtcct ggagtcagtc
    20041 ctgtaagtgg aaggcagcta aagggtgaag gtggatactg tgtgtggtag tccgattatc
    20101 tgaggcttga tcgatgcagt ccgtcggcca ttgcgtttcc ggatggtgat ctgcttttgc
    20161 aggatcgcct gttcccaggt gtcctctgtg gtgtgaacga cagccctccg agtttgtgac
    20221 agcgcactcc agatgaaatg acagaagtcc cagcgtctcg cttcgtctgg gagctgggca
    20281 atgtggtcaa ggagggagcg ttcccctctc tcattcttat cctccgcaat gagccagccc
    20341 tctccgcttc cgttaaacca tcgcagtgaa atttgccgat gctctggacg gatgaaaata
    20401 tcatagcggt aggagccgcc agtccacttc gtctgtttgc taaaactgca gggtctgcct
    20461 ggtccgaagc gtgcggagca tacgtgtgtc cattgagtgg gatcaacttg gatcgcaaca
    20521 taccggttac cgtaaggcgg atggagtaga tatccagatt cttcaatcct catcgttgga
    20581 atgggaggtt ggttcatacg tcctcatgtg tcgaggttga aggaatgcgc tgttcctacg
    20641 tgggtgataa ggggtggaga tagcgtcggc agagatcgag ggggtatccg gtacgcgagt
    20701 gatactctgc cagcgtttct tggtgccgtg gaatgtcggg aagtggaatg ccagagagga
    20761 tctggtattg gtgaagcggg agtgcgggat tgtcgctgaa ctgatagatc tttcttccgt
    20821 gctgttcaat gaaagccacg caagtgtctc tgctgaaact ccgagggagc cgataccgtt
    20881 cgagaatgct cgcccaaagt acgccgtgcg catagagatc aaagcagagt gtatagagaa
    20941 aatgtgggtc cccaattggc aggaggggca cgtacaacga tccgtctggt tgaggggtca
    21001 cttctcggac atgggcatag acaattggcg gttccggttt atgtatcgac tctgtttcgg
    21061 ggagagactg ggctgtccgg atgtgcgcgc attcggttcg tcggtaggtc caatgtggac
    21121 aggtgcattg ccagtcattg gcctgacttc gcgtgacggt atagcaaagt cccggcttgg
    21181 aattcgatgc gatggagaag gattcggcgt gaggcataca gccttcctgt gcgacgtgag
    21241 cttgactggg ggggaagcga atcagactcc tatggcggca ggatctgtgg agcggctgct
    21301 gtctgaggca tgagatgcgt tggtcccgtg atgggaaagc ctcacagcct tctgacctac
    21361 cgagatgcgg tgaaatcaaa aaaggaagcg gtagccggtt cagtcggagc ggggttcatc
    21421 caccaacttg gaccttccaa tgatggcgcg tatgagactg taaggtcgat acatcgacca
    21481 cggtttctaa gttggtcgag gatgtcctcc tcactcgcaa gatactcgct atcggtgaag
    21541 gtcttacatt gaagatgggg catcggtggg gcgatcccag gaggaagcgg agcgggtggg
    21601 agatacccga gaaggtagag caggacaacg gtcgatatgg ttggcatagt gcctcatcaa
    21661 cttaatctcc accattctat cgaagcgtgt gaacgtgggc aatccgacct ttgggcttag
    21721 ggatgggggt cgtcttccac tggagaggct gacaagctca cgtcggtgtg aggccgttca
    21781 ctttcctgga caaggatctg caacatggct aggtcggcac gttcttgttg tgcacggcct
    21841 tcatcgtggg tgagacgaga tgtggatgcg cttccaagta agtgtcgaat gatgttcgag
    21901 ataatctgac gaaggctact ggctaggggg cgcatactgc cacagtaagt ctagagcgat
    21961 ttggtacagg ataggctaca agacgacgcg cttccatttc ttgatgcggc catcgatgcg
    22021 tgcgcgctca tcatgtgtga gctgtggtct gagcgtatct gcgagttcct tggccggttg
    22081 gaattgatgc ctggccgcaa gcgtcgccca gtagtaggct tcaggcaaat cttgccgttg
    22141 gttcgccctg ccttccgaat aaatggatcc gagctcgtat tgggcaaaag ggaaatcggc
    22201 ctgtgcggcc ttccggtacc agcgcatggc ttcgtgacta ttgggaggac tcttgaatcc
    22261 tacctcaagg tctgcaccga gaagcagctg tgcgtccggg agtccgagat ccgccgcgcg
    22321 ccgtagccat gcaagggatt cccccgcgtt gggatttgga gaaaatgaga ggcagagcgc
    22381 tgccagcgca tcggtctgcc ccgcccggtg aatccagctc cggatgagcg cctctttgtt
    22441 gggctgcgag gctacctcca tttgacatcg cgcgaccgca ggcaagtcca taggttgagc
    22501 tattacgact tccgaaccga ggagcgtcat gccgagactg cagccgattg ttatgaggcc
    22561 gtggcgactc cagcgttgta ctggtgatag aaattcactg atcactgaaa gaggtaagct
    22621 ggaagtcagt gtagccgcca ttgtgagagc aactcctttt tgaacgcaac ccattcttga
    22681 tgacacccat gctgagatga cgtcgtccct gcgtgtgtat taccggatgg gcagtcctgt
    22741 tcctggattg tggggagctt aggcagcagg atcgaggggg aggaatctgg tggctttcac
    22801 ttttatcccc tggtcattct gatcgagaat cccttcgagc gcgatgacgg tgccaggttc
    22861 cagttgccct gattcgtgtc ggtagagact cggaaaaata acgacttcga gtgtgcgatc
    22921 ctgatcttct aggtggacaa aggccatggg ttgctgcctt ttagtggtga tgcgtttgac
    22981 tcgggtgatc aatcccgcga ttcgcacttc tttgccatct gggaggtcag gaagctgagt
    23041 taacggaaca acttggagac gcttcagttg gtggtcatgg actcgtacgg gatggtgact
    23101 gacatagcat ccgagagttt ctcgctcgtg gtccaattga atagatggag cccaggttgg
    23161 tatttggggc agcacgcgag tttctgactc caccggcaag aaatggagca gagtttgttg
    23221 tgtgacgttc cggtgagatc gagtaggctt cgctggcgtc tcatcatcta gaattgcgga
    23281 tagttgtgcg cgatggtcac agagcgtgtc gagggcgccc gatttaatca aggcatcgag
    23341 tacgcgcttg gtgacaatgc ggcggtcgac gcgatcgcag agatcaaaga aggaagcgaa
    23401 ggtgcctccg gttgcacgag tccggatcaa ttgtgtggcg agatgttctc ctacgtgctt
    23461 gattgcggcc agaccatata gcatggcccc gtccacgatc gtgaatccgg tctcactccg
    23521 gttaatgtcg ggcggaagga gcctgacgcc catttcccga cattcactgg catagagaga
    23581 gatttgctca tggtcaccga aatcactggt catcaacgca gtcatgtaag ccagcggatt
    23641 gtgagctttc agataagccg tccgataggt gagtagcgcg tatgcagcgg catgtgattt
    23701 gttgaatccg tagccagcga acttggtgat catctggaag agttcttcgg ctgcgtcacg
    23761 ctctgtgccg agtccgacgg ccccctccac aaattgtgta tgcagggcgc gcatctcctc
    23821 tggtttcttt tttcccatag cccggcggag aatgtcggct tggctcagtg agaatcctgc
    23881 cacagtgctg gcgatggcca tgatttgttc ctgatagacg atcacgccat aggtagtcct
    23941 gagaatcggt tcgagggcct cgatgttcga ggcgagggga cattgcctgc gtttctgctg
    24001 aataaactcc ggaatcagat cgaggggccc cggacgatag agcgcaataa tcgctgtgat
    24061 atcatcgaac cgatcaggct gaaattcacg aaggagctcc ctcattccgt tgctttccag
    24121 ctgaaatact cccgtggtac gtccgcttgc gaggagggcg aaggttgtgg cgtcgtcgag
    24181 cgggatgtcg tccagtgtca acggacggga tccaggagat tgctgattga tcagtgagat
    24241 ggtgtgatcg atcacggtga gggtctttaa tccgagaaaa tcgaacttca ctaggccaag
    24301 ctgttcgagt tcccccatcc caaattgcgt cacaatgtcg tcgctcgtcg tgcggcataa
    24361 tgggaccgtc tccagcaggg gggtattgga gatgaccagt cccgctgcat gagtagaggc
    24421 gtgtctggcg agcccttcaa gggatccggc caccgacagc aggtttccga ccgaagggcg
    24481 cgaggcagac aaggcactta atttgggctc ttcggcgagc gcctgtgcga tcgtcatgtt
    24541 gggctgagac ggaatgagct tggcaatcgt gtcgacttca tcgtagggaa tgtcgagaac
    24601 acagcctaca tcgcggattg cagctttggc gcctagagtg ccaaaggtga tgatttgtgc
    24661 gacatggtct cggccgtatt tctccgtgac atagttgatc acctcctgcc gcctcttcat
    24721 gcagaaatgc atatcgatgt ctgggaggga aactcgatcc ggattgagaa accgttcgaa
    24781 gaggaggtcg taggcaagcg ggtccaactc tgtaatcccg agcgcgtagg ccaccaggct
    24841 acctgcggcc gacccgcgtc ctggtccaac tgggatgcgc cgggagcggg caaacttcat
    24901 gatgtcccac acgatcaaga aatagccggc gaagcccatg gagcagatgg tgaggagttc
    24961 ggtccgcagg cgtcgttcat actgaggttg gtctagaggc cccgagtggt gggcgaggcg
    25021 tgccgacaac ccttgttggg cgaggatttc aagaaaactg tcgagggtgt gcgggggagg
    25081 cacgttgtac cgaggaagtt gtgtccgatg cagaggaatc tcgagcgtgc actgttcagc
    25141 tatcagattt gtgtgtgtga tggcttgagg tagctctcga aaggcttcgg ccatctcctc
    25201 ggaggatttg acgtacagtt ggtcggttcc aaaacggagt ctggttgtat cagtgatcgt
    25261 agtgccggtc tggatgcaga gcaataagtc atgggcgtct gcatcgcctc gtttgagata
    25321 atgacagtca ttcgtggcga ccaacgggat ccccagctcc cggtgcacgt gaatcaggcc
    25381 ctgattgact ctctgttgct ggtgaagtcc attggcttgg agctcgagat agaacctatc
    25441 aggaccgaag atgtcgagaa agtcttgggc ggccacggac gcggaagcga actgttcctg
    25501 gtggaggagg gtggggattt caccgtcaag acagccagac agtgcaatca agccagaact
    25561 gtggctgcgc agcaaggact tgtctatgcg gggtttgtag tagaaaccct ccgtgtagcc
    25621 gtgactgacc aggcgaatga ggttttggta tccgagactg ttctgggcta gcaggatgag
    25681 gtgatagaaa tcacggggtc cctctggggg ttttttgtct aacagactgc ccttggccat
    25741 gtagacttcg cacccaatga tcggcttgat gcctggcgct cgggccttgg tgtagaaggg
    25801 gattgtgcca aagagatttc catgatcggt gatcccgacg gctggttgct tgagccatct
    25861 gacgtgttca atgaggggat ctagaagagt ggcgccatcg agcaggctat aagtggtgtg
    25921 cgtgtgcaga tggacaaagg gtgttggcat gacactccag attgatggga cgagttttcc
    25981 aggcggggga tgagagtaag cgctcgttga gaggtggtgg cgcggttggc cctgagctgg
    26041 gaaggaattg tgtacgtgtg ggcgtgggat tcgtcaatac taattaggcc taaaaggtaa
    26101 atttaaatct gatagataaa tacagtcctc ctcatcaaag gagcgaggat catgacgacg
    26161 gaacaaaaca tcatcagaat caagctgggc gtcttgaaac tgtcgaagca cctgggcaat
    26221 cgatgaaagt cttgtcaagg taagggatac agtcgagatg gattctatcg attcaaagat
    26281 ctgtagacga cccatgggcc cgctgtgaaa tgcagtgtca tagctctgtc tcactatcct
    26341 cacagagctt gccgcattgc tcacacgctt cacccggcat aagccccgcg atacatgggc
    26401 ccgcgcacgt tgtgtgagat tctccctgtg ctgcagcgag agcatcctgt gcgaatctgc
    26461 acgattcccc ctggtcctct gcacaacgga ctaagagtcg tagttgttca tacagtttcg
    26521 gagctgcagc gatcaggtgt gcgttcgcga ggacctccct tctgttgttg cagggctcag
    26581 tgatcaccgc aatcgttaaa tccgcatcct cgtcctctcg tggcgtgaca atagttaccg
    26641 gcccattgtc gctgagtttt attcgccata gtcctggcat ccattggggt aggggtgcgt
    26701 ctgcttcttt catactgacg cctttctctt tagtgatcat gcaccgtcgc tcgatattgg
    26761 cgggctagcc cattctgctc gattgggcca cgcatttacg tcaggctaca cttgccgcgt
    26821 gacagtgttc ctataggctc ctccagtcgg gacggtgccg ctccctccgc gagtgccgca
    26881 cgtgtccggc gagtggcgtt cagtgtggaa gagaacgccg ttttcgcttt aacatggctc
    26941 caccgagaga ctgaaggcat caagagtgtg catcgaatgc gggcagcgaa agagatggat
    27001 agcgattact tgcgagatgg gggaggggcg tcgactctct cgggagggtt gtgtggaagg
    27061 acgaccaggg tgccttggac tccggctggg acgatgtacc ccttctgatc ctggctttga
    27121 tgtgccaatg acaacgggac cccttgaaac ccgtccgttt cagaaatcac cgttccagtg
    27181 aagagtctag tttcaaatcc tctagcagta cggcacgtga tggcatggct tcggatgtgg
    27241 atccgacctc gatctggatc tagccgggcg tcgccactaa tcgtgcagtc ttgtacggga
    27301 gcattaaata acaccaagag tcgttcgtcc ggcgccagcc gtagccggat cagtgaccat
    27361 ggagatggca cgaattcaat cgataaagga attttggctc cactcaagac ttcaactgca
    27421 aaccattgat tcatcagttc ctgggggaga ggctgtgcgg cgtgcgtcag acttgccgta
    27481 gaaatccaca aaataccgga aaggccccag cgttgaatcc aggtgctata agcgtacggc
    27541 atttccaaga acgaaagcat tcgatgtgga tcgtcggagc ttgatctact ttgatcgaag
    27601 tattgtcatc ttgtattacc gactttcttc agcggtgtcg taggttcatg gctaggaagc
    27661 cctccccata gacttgtttt ggtgtaagcc tagtgcacgg gagatttccg tcgaagtaat
    27721 tgccagagag cgcgctgagc cagtatccat atcgatatag gttgcaaaac ggcgaaggtc
    27781 tttggtgatg acgagtcgaa gcaggtagtc cgccatgtgc gaggcaatca tgctattgat
    27841 cgtgagggat tgcgggtcac ttagtgcaag ttcagcacat gaccgcggtt gagtgttgac
    27901 ggggcgacgt tctcgtttga gcaattctgg gtgcacaagc gctggcgcgg ggagtgactg
    27961 gcacacggtg cgcagctgaa agctctcgtc tggtttaaag tctttgatga cgttaccaac
    28021 taacacttgc ccgtgggtgg tgaggttccc accatcaatc agccatgctt gggcatcgtt
    28081 tctggtgttc aatcgcgcga gagcttgatg gaggctacgc ctagcccttg ctgtgtcaac
    28141 gcaaccgaga agaatggtca gggtgtcgta ctcaggctgt gccactttct ccgagaaatg
    28201 ccgtggaaac gcgcgaatgg gaatccccca agcggtagag attcgaaatg ctaaggcctc
    28261 tgccttattg tgtccgattt cagcctgaca gaagttttga cgacgtaggt tcttctcttc
    28321 aattgtgtcc ccatcataga aagtgacctt caccgtatca aatagacgga aacactcccg
    28381 tgcgaggcgg gcgacgtgga taccaagaaa cgctccgatg cccccacatc cgatttggat
    28441 gagatggagg cggtggcact gcttaggaag cacattcacg gcatgtacat gactaaggtc
    28501 caggtgtgtg ctggtgtttt gttctatgct gcgattccct gcagtgacaa cctgtcgcga
    28561 tgacatctcg cccctcattg tctgtatgtg tcctgaagtg tgggaggtag ctctgcaacg
    28621 aactccgctg ggattgggat gaagtggcca tacagcccga cacgaaatgt aatggacggg
    28681 cgagtggtca accatccaca cacaccgtat attcgaaacc ccgtttcgtc ttcattgtcc
    28741 acgacagaaa accgtggagc catggtgtgg tgtgagtgaa tctcgacaat ggcacgctcg
    28801 taagatggac agcgttcagg ggtgctaggc tggactgaga agggcgtacg aatctggtct
    28861 ggaatagtca tgcaccaatc tgtgtcgtgt tcaaaatgga atagactttc tacgggaccc
    28921 gccggctctg cgcaggctcc tagtgcctct tccatcatct tttggatgag ccgttgtgga
    28981 attctcggaa tcgtgagcgt gaggctgcct gcaatgggag gaaggccggc aattggatgt
    29041 tcgacaattg gaaaaaacac tgacagttct ctacgtgtgc cgcgaataaa gaccccgttg
    29101 ccagccagaa catagtcgta aagggtggaa ttgtcggcgg gaagtagaca actcgcaatg
    29161 tggtgtttaa taaaaggaga cgtcctcgtc atatgtgatt accccttccc gctctccatc
    29221 agttgacgaa tgatggcggc aattgagagg tgagccggct caagatcatc gagaggatag
    29281 tcttgtgcct cagttgagtg aagtttcgcc aggaagggca ggatattgtc cgggtgggtg
    29341 gtggatttcc cactcgcgag atggtcggaa aagttactat cccagaatag tcgccagctg
    29401 tttgcaatcg tgttgccgtg ggcaacccgt ggatggacgt ttccgaaaca gatcgcgcca
    29461 tcagggtaga catttgcaag cggtgccttg aagagttttg ttgccgcgta cttccatagc
    29521 ttgtaggccc agacgtaata acgggtcccg taccccagaa acaacaaccc aggcatcggg
    29581 acggcaagca ttgtgtgtgg ggcccctcct ccggaaagtc taatggggtc agccagtggg
    29641 accaggtatc gaccaggggg aaatcggagg attagccatg gacctccggg gcctatgcca
    29701 tagcggcaga catgttctgg gagccagtcg gaatctatcc cggctgatga gaacgcattc
    29761 ctggcggcct ctggtgaaat acattttcgg acaaccgctt ccccatcctt gtattcaaga
    29821 agcaattgcc cgccgatgaa gtgcagagac gcgtcgtgcc ccgagcttgc atctctcatg
    29881 tttgcgcctc atgtgttgca gtacaaagat ctgaccagca gctgaaaatc gtttcccatg
    29941 cggacggatt gtcgagtaag gctttagtcg tgctattcat ggcttcttgc atgagttgcg
    30001 cccgtttcca ttggcgagcc aaagctttga gatcccggtt gttccattgg tacgggtgat
    30061 ctggtgactc gcagtcatgg ctgatgtcaa ggaacagaga tccggtgcag tggccgagga
    30121 tgtcgatgac ctggggaaat cctgaaatgg gaaagcgccg tctggcacaa cgtctcctga
    30181 agtcaagaaa ttgaacgttg caccggtgct gaggattcaa aaagcatcgt ggaattgtag
    30241 cgtgctcgct gagttcttct tgaatattcg tccaggtggg ggcgtaggaa tagtgtccca
    30301 cccaggccgc tgcgatacag attggaagcg gtacctcttc gaattccgac tcacaatcat
    30361 aattttccaa atgtatggga atgaatggga agcggtctcg aatccattcg gtgtcccagc
    30421 tatcgagcgg gaagtagtgt tgctcgacta gtttaatgaa ctcttcttga agctgttctg
    30481 cggcctcctt gatgcttcga tgcggaaggg cggcggcggc agtgcgaaac ctccgtggaa
    30541 agatctcatg gaacgtgttg acaatacaaa agtcgcgctc tctctcctcc agatattcga
    30601 ggcatgaggg gaccgagata acctctgtgt ttttgaggga tgcgagaaca aggttcaacg
    30661 gtgtggaggg caggttaaaa cccaggtgga aggatgcggc tgggcgagcc aggagtgctt
    30721 gtgagtcgct ccttaatggt agctgtttct ttgtattcct tttcaccatg ctcaatggcg
    30781 agacgcagct tttgctgcgt gaggaatatg gattcaaccg ttgcctgggt tggctgggac
    30841 tcagcttggt atgtctggta gagcgtgagg gcgggattga cgtggcgggg ggactcgata
    30901 agctgacgga ggatgacatg gtaatttcct ttcgttccag cctgtttcgt gaggctaatc
    30961 gttgtcatcc cattcgtgtc agtagttcgc tgaatggtgg catgggcggc ttgtgggaag
    31021 aggggggcga tcgctttgcg aaccgcatcg tcgctgatgg cgatttcggg gggcaattga
    31081 atttcttgtc cttcaagttg aagcgtaacc ataaggctcc tttcgctact cgaataagcc
    31141 ggcttgagga agggctgtgt ttgaggggga atcgtctgag tgttgttcgc gatccggttt
    31201 gggtgttggg gccacggacg tggttttcgc ggcggtcggc ttagcaactt tggcttttct
    31261 ggcatgccgt tccgcttcgc gttttttctc ctgttccagg cttctagtct gttgctcggc
    31321 ggctcttgcg tcccgctgtg ttttccgtgc cgttagcgcc gcaggaaagg tctgctccca
    31381 acgtgtgagg gcctggctca gagattccgg aaggggcagt aagtcgttga atcgtgcggt
    31441 agtcagctgc ggcgggaggt tgtgacttcg tacgccgatg agtgtgaggc ggccgtcagg
    31501 gtggcggtcc gttgggagca gcgtcatcac gatggagatc gtcgcgcgct gaaaatcatt
    31561 ggagtcctgt tcgaggctaa caatcgggtc ctgttcatct tcgtcgtctt cttcatcgcg
    31621 tcgtgggggc ggaggatcgg gcaccgcctc ggttttcata ccagctggtt cggcggctag
    31681 cctgggcaat actggggaat catccccact ctgagacaga tcagcagagg aagcatcggt
    31741 aggtgtgctg ttcgtaacca cttgggtgtg tggaagcaaa tcgactgtgc caagcatggg
    31801 gggcctcatt gtggtgtgca caacgcaggg gggatcgtga ggcggtggtt attgttttgg
    31861 taggcgctgt ctcaaggact ggagtgggat ggggcgaagc tgttggttga gaacgtcgga
    31921 gtatctttgc aaggggccgc gtaggtcagg cgatcagggc atgcatggat tgtacaaaat
    31981 gaagtgtggt ccgatagatg ggcgcggcat gccgaagtcg atgaataaat tcttgcatgt
    32041 tgatgggatg tgcgagggct tgtgccaatg gcgcacttct tgtcgtacag gtagaagatc
    32101 cctcggagcc tgaaggtcca ggcatcagta ttggagtcag cacaatagtc ccggcttctc
    32161 tcgacaggcg atgcgcaagc ttgtcgtaaa actcggttcg gtctctgcgc cattgtcggc
    32221 gccgtctcac tacatcgatt cgtgtgtccc gaatttgcga gagtatttct cggattatgc
    32281 ttgtaagggg agcgctgtag gccttttcaa ctgcctgatg agcaatgcgt cgaagcttct
    32341 ttctgctcag tggcacgtcg ctgtgaatgg gctccttgac catatcggcg agtgcctcac
    32401 caaatggaaa atgaacaaat agttgtttga gggcgaggtg ctgaacattg agggcttccc
    32461 tggcttcgta tgccgcaatt agatttttta gtatccgcgc gggaaagaag acggattcgt
    32521 gtttgcccgt gtcagatacc cattctccca ccctaacaag gggcttcgcg tcatttatga
    32581 gtcgatgatc aaaatcaagg gcgatcatcc cgtatttttt tggttccgtt tgctgggtcg
    32641 gcggtacgat tagcgtgaat tgaaggtgcc actgccatgt cgtgtcgttc ctgaggcgtg
    32701 cattggaact gtccaatttg acgctcgcct gtggtcgctg ttgtgaacca atgagagaga
    32761 cctgtttgag aagagcgtct ttgggcagaa gcggagtaag gatgatttca aacggaattg
    32821 ggacgttgcg aacgaaaagc tccccctcgg ttctgcaaaa atctcgttgg ccccggtctc
    32881 gggggtcggt gtcctctgcg agttgcgagg ccgggttgag tgtaagtaga cgattcgcgg
    32941 caaatgagga gcccgactgg gtcatgtctg cgcgagtcaa cgattggatg cggattcctt
    33001 gcgaaccgaa gttgtgagta aagaataacg aggtcggtgg atctgtttcc ttgctaggtg
    33061 gaagatttcc gaggggcact cggcggatgg cggttttcat tcgttcgagc acggcattgc
    33121 tgtgtgaacg agacagaggg cttgtgcgtg cgagttggtg cagagtgtca ttcagacgtt
    33181 tggtgatctg ggaaagctcc tgcttcatgc tctcagggta aggtagacat cggtcgatat
    33241 tgtgatgttg ggaggcttcg tactcagccg cagctgtgcg ataggtacga gaaagggcaa
    33301 gaagagtcgc gtcctcgagg gagtcgttct gcccacgcaa ttgtttgatg cactcaaggg
    33361 gatcgccaat cgaacgtgtg ccgccagctg cgcgcgtcgt cgttcgactc gtttgaagga
    33421 attgtttgtg aaggcgctcc tgttggcgat aggcgacttc ataaacgtcg accatcgcat
    33481 tccacagcga ttgcatttga tcagcttctt ctctgaccgc agccggcaag gcagcatagg
    33541 aagaatatgt gagatatgcg ggatacgtat agacccgtgt tagcgatgtc ctaatgccgg
    33601 cgggcatggt gcaatctcct tccaagactc tcgggtgcca gtgcgacgca ttgctgtgct
    33661 tcgcgcatga ttggtttcag gtaactgtag gcgacagtgc caaggagatt ttaagtggtt
    33721 tccccatctg agcgtaggag catttgcctt atgtgattgg ggttccccct aacggaacgg
    33781 caatggccgt atcggagtag gtgaacattg gaagggcgag gtagattccc ggggtgcgct
    33841 tttcaaagag ataggatgca agtctgactg tgcatcgtgg tgtttgtgtc ggatttttag
    33901 ctggttgtgt tggtgtatgg aatgtccttc actcctcgac gtgtctatgg ccagggtctt
    33961 tgcagcattg aagcgttgat caagagatgc ctgaatggaa ggggttgcga ttggtgatat
    34021 agagcattcc cttttgcgcc cgattattct tacatcaagc cacgcaggta ataccgaaac
    34081 ggtgatttca atagaacatg tcacaaggcg ataccatgcg atgggctgac caatacaacg
    34141 ttatgcggtc gatgttattg tcgtcccccg cgactcattg gtgggttcgt gatctgattc
    34201 tccgcctgga cgccaaagac ccggttgacg tgttgaatgg actcgaggtt gtggcaagac
    34261 tgatgcggat gcgtcatgag gcagttcgcg ccggagaatg ggatcagttg gagtgccaga
    34321 aaccgaccgg ggacgacggg atatcaatga aagactgaaa caaagatcca tagacgtgaa
    34381 gatatgacct caggtgacca gggcttttag accgtgatac acgggcgggc ggttcttggt
    34441 cccgtcgaaa gaggaatatg actgagtcga gagcgtgagt acactttgat ctatgtccat
    34501 tgccgaactg ccaaatagcg ttttcgaaga ggcgcctctg tcctgtgtgt tccattcagc
    34561 acaagacaat ctatacgacg tgggaaaaat tgttgggctc tcaatgcacc attgccatgt
    34621 cgagagtcag gccgaagtga cgccggggat gacactgtcc ttattcgttg tcctaccaac
    34681 aaagcgtcgg gctctcgtgt tggatgaggc tttggtgacg tgggtacgga cggaagaatt
    34741 cggagtacgt ttgaaaacga ttccaggtga agatgccgct cacttggagt cctacctcgc
    34801 cgggcataag gtggacgctg gcttgtgttg aatattcatc gcattgatca cgaggcctcc
    34861 cacacgcact gttggcgggt gactgtacaa cgtcgcaccc gcatttacgt gcgaagcttc
    34921 agtgacggcc gctacggcgg agctgagcag gcgttggttg cagccacgac ctaccgggag
    34981 gcactgatca ggtccaatcc tccgctgagc aagccagtct actgtgccat tctcaagaag
    35041 acgaatcgct ctggcgtttc agggctgagc cgtattgacc gatgggaatt ctccaagggt
    35101 cgccgcgtcc atcggctcta ctgggacgtc caatggccga tcgagaactg tcaatcccgg
    35161 cacaagaagt tttccatcct caagtatgga gaagatgggg cgtatcgacg ggccctcaag
    35221 gccagggcgg ccgctctcaa ggcgatctcg tcacagacgt tttccccatt cgcgtcgaga
    35281 ggtgcggcaa acaatgcagc tgcataggag ttgtgggtcc agattgccct tcagcgttcg
    35341 gtttgtaggc ggtcgataag tgcctgaatg gtcagcgggg cattcccttc ggctcgattg
    35401 ttgacgagga cataggtcga ccgattctct gctgtggctt cctgtataag actcactgta
    35461 tctttgcgca tctccggtag ctcccccacg attttgtcat agggctccgc tcgcttcttc
    35521 gctgcctcgt aagtcatttt taatggggtc aagagccgaa ttacggtgaa cggggccgtc
    35581 atggtcttca tgcgttgatg ctgttcggcc agcgggggca tataggacca atgattgtag
    35641 acatgagcga ctccgtgtgc cctcagcatg tcgtggtagc gtggcccgag caggccagca
    35701 ttccgtatct ccacggcata gcggaattcc gctgagacgc tgtcgaagaa gtcatcaagt
    35761 ttcccgatga actcttccgc cggcatcccg tgccgttgga actcgaagag gaacggcccg
    35821 gtgtttggcc cgaactgcgc gtctcgatag ggctgcagta cgagtttggt gaaggcgtcg
    35881 gcattgagaa agttggggtt cacctgccct gcgcgcgagc catagcgagc atgttttgca
    35941 taggtgggaa tcgtgagttc ttcccacact ttgaagcaca tctgaaaatc ttctggaatt
    36001 tgcgtcaaat accgagtgag ctggtttgcg gacggggggc gataaaaagt tgcatcatta
    36061 cctaccgtgc ggaagagcgg ctggcctctg tagagatact ggcaaaattc tcccaggcat
    36121 tcacgggtaa aggctgtctt agtgtacttc cgtgtataga tttgtccctg ccagccctca
    36181 taggtccaag tggaggttcc aaagcgaatg aggggagaaa gtggcatgtc cctcagtgta
    36241 cggatctcat gcgtcttcct caagtaacat agtctcttcc gtgtcggacg cggataaggg
    36301 tcgcaattgg gtaactgtaa ccgtcacggt cgcaaagtgt tcttcgacca cgcctgtgac
    36361 gaggtaggcc tgatttgagg cgaggaggtg gcagtactgt cggtaggtgt cggggaagag
    36421 agttgcgtca tagaggctgg tctgatcctc gaaggtgaca aattccatcg gctcaccttt
    36481 ctttgtggac accactttct ccgtcaccat ccatcccagg agcgtgactt ccttgccgat
    36541 gtgctgggcc aaatctcgtg ctgggagagg cgacaagccc gcgaccacgt cggtgaagag
    36601 ttcgagcgga tgtccgttga ggggaaagcc gaatagctcc agctcgtggg ccagcttccg
    36661 ctgagcagag tactccgctg gaatcggcag ataaccgact ggttttcccg attgatcggc
    36721 gaaaagtctc cagatcagtg cgggcctggt cagttctcca gccacggaat cgaagcagcc
    36781 tgccttgatt aataacgtgg tttgcgcggg ctccggctgc agacgatcga gaaagtgctg
    36841 caaggatcga taggggccat gggcttctcg atctgccacg atgtgctgtg cggagtcttc
    36901 gcgcagtccc ttgagttgca tcaatccgac ccggatggtc ctgccggatc cgcgataggc
    36961 ccaagcactc gtgttgatgt cgggcggaag catggtgagt cctattcgtc tgccctcgga
    37021 cagataggcg aaggcggagt agtacccgcc ttgattgctg atgacggcgg cgaggaactc
    37081 ggcggggtaa tgcgcccgca ggtaggccga tttgaaagag acctgtgcgt aactggcgga
    37141 gtgcggctta cagaaactgt acccggcaaa gctcatcatc atggcccaca cctgatccac
    37201 caccgtgcgg gcaatctgcc gtgccgcggc tccggcgtaa aactgccgct gatagtcccg
    37261 cagttgccgc tctttgtgtt tcttgctcaa gatcttgcgc agttgatcgc cgtcgtgcat
    37321 cgagaacccg cccagtgcca tggccacctt catcacatct tcctgataca ccatgatccc
    37381 gtgtgtttcg gccagcaccg tctcgagcag cgggtgtaag gcgcgatacg gctgtccgtg
    37441 cgcccgtcgg acgaagtcat cggcaaacac attggccgcc ggacggatga tggaggagac
    37501 ggcaacgaga tactcaaaaa cgtctgcctg ggcgcgttgg ggcggcggca tggtcgtcca
    37561 gagtttcttg agcaggagcc gggtcgcggg agactccacg taaaagcagc ccatggtgtc
    37621 cccgcgccga atcagttcct gcgtagcggt gtcgctgatc gggtcccagg cggcatagtc
    37681 gatggtgcgg ccgccgttgg cggcaatggc ggccagcgca tcacgaatga cggcaagcga
    37741 gcgattgcca agcaggtcga ttttgaccag tcccgcatct tcggcctggt ccttttccca
    37801 ttgaatgacg ggccagccgg acgcagaggt ttccaccgga acataccggc gaatctcatc
    37861 agggacgagc accacgccgc cgggatggag cccgagatgg cggaaatgtc cctggagctg
    37921 tcccgcccag aagagaatgt ccagccacgg cgggttgagg cgtaacgcct tgcagacctg
    37981 tgcggcccac gtctgcaggg tggcgtcagt cgtcacctca acaaagtcgg ctcgacggtg
    38041 caggagatcc agggccttgc cgatttcagc cgccggcagt ccatacacct tggcaatttc
    38101 ccgcatggct gcccgcgctg ccagggtgtt ttgattggcg accatggccg cctgatgtgc
    38161 gccatagcgg gcaaacaccc attcgagaat ttttgggcgt tcgtcccagg gaaagtcgat
    38221 gtcgatgtcc ggcgggtcat gtcggccggg gtttaggaat cgctcgaaca gcaggtgatg
    38281 ccgaatcgga tcgacgtggg tgatgccaag gcagtaggag acgatggagg cggctgccga
    38341 gccacgtccg catgtgcgag gcgcttggcg aacgatctca tcgacgacta ggaaataatc
    38401 ggcaaagcgc ttctcacgaa tgaccgtgag ctccttctca atgcgtgcgc ggacgacctc
    38461 agagagcacg ccgtacctcc agacggcgcc gttgtaagtc ttggctcgta acgtctcgac
    38521 ggcgccgtct gcagacatct cgcgaaagga tggaaagatg gtggtctgga agtcccaagc
    38581 ggtggcacac tggttcgcga tgcgacgtgt attactgagt gcctcaggga catgtggcag
    38641 ggcgcgagcc atgacagagg agggcatgag ccaatgcgag ggtgcgcaac agcaatccgg
    38701 tcgaagccgg gagagcgtcg tgttctcagc gattgcacgc aagagccgat gtgcctcaaa
    38761 gtcggttgga cgcagcaagg ccgcgcgcgt ggtagcgaca ggagggatgc aattctggcg
    38821 actaaatgag acggccttct gaagatcggc gccaggggtc agctcaacgt acagatcttc
    38881 acccgactgt gcccgccacg cggtgagggc ctgaaaatcg tccgacagga tgacgaggcc
    38941 ggctctataa cgttgtatgg tctcaataaa ttcgaacggt tcatcctcat gacgagctga
    39001 gagcagtcgg cataggttgc cgtagccttc agggctcttg gccaaaagaa cggcgcggtg
    39061 ttgggcctgt atgatttccg ttcccaggat gggcttgagt cccgcgcgct tcgctatctc
    39121 aagaaatcga atcgccccat acaacccatt ggtatcagtt agggcgaacg tatcgtgtcc
    39181 ttgggagcga acggcctcac agagggcttc aagcgtcgag gtgccccaca tgggtgaata
    39241 ggaggaatgg gtgtggagat gcacgaagtc gggcgacatt cactgcgccc tcccgtagcg
    39301 aattgcttgt tcgccgaacc gttgatgaag gcgatcgaga gccaaggcga gtcgctggct
    39361 ccggtcgcga gtccgttgtt cctctggtgg tcgtgcatcg aatagcgatc cttgctcggc
    39421 atagtcggtg agttcggcta gggtgaccgt cattcctcga agtcggatgc gccgctgaaa
    39481 gctacgctga aagagccctt ggagcagtcc ggagagatcg atttcccagc aggtatccgg
    39541 catcacccgc gcctttgtgc tcacttcgcg atgatcgctg tagcggaggg tcaaggagag
    39601 tccgccgcac atccggcgct ggctgcggag cgtgcgacag agttgttgca gacaatcttc
    39661 cagtcttccc tggagccgcg catcatccac ctcatcgggg gtgagtgtga tggtgttgct
    39721 caggctcgga tgggcaaccg gtcgaaggac cggtgtgggg tcgatgccct gcgcccatcg
    39781 ggagagttgt ccggcatagg tgccaagcgc aacttcaagt gcttcgagag ggctgtccgc
    39841 gacatctccc aatgagcata ggttgagatc atctaatcgc ttcagtaccg tgcgcatgca
    39901 ggggcgatgc acgccgggca aggctcgaat tgacaacgga gacataaaga ggcgctccga
    39961 tcccgagcgg acatcgtaga gttcggaagg ggctatcaga gtcgcggctg tctgtgccac
    40021 gagtttattg ctcccgacgc cggccgcgcc ttcgagatga aactgtgtga gcacctccgt
    40081 ctggatcttg gttgccacat cgcaggccgt cccgaagagg cgggtggtac cagtgaggtc
    40141 catcaagaga gaaccaggaa gtaacggctc ccaggttggg gcataccgct ggatgacgcc
    40201 gaagatcgaa tgggtggccg tggcgacctg atgagggttt gggagcctga cctgcaggga
    40261 cgggcaaagt cgtcgtgcac ggtcgagcgt catgccgatg tggaggccgt ctcgttctgc
    40321 ctcaggagag atctcacgga tcagtgcgcg aggggtatcg agtttagcaa ccgcgagcgg
    40381 acgggtgcgg agctgcggat gacccaaccg caccagcgcg atttccagcg agggaatggc
    40441 aaagcagaca atctgacgat ccatggtctt cgctcatgcg gtccgatgag cgctgttatc
    40501 gtttcagatg ccggatgacg ccgacgacaa tgccttcaat gtgaaacgtg tcggtttcgt
    40561 gcaccaggat gggcgtcatg gtgtcattag ccggatgcaa ctcgatcatg ccggccttgc
    40621 gatggtaggt cttgatcgtc gcttctccgt tcaagagcgc gatgacagtt tgtccgtttc
    40681 gagccatatc ctgtttctgc acgacaacca cgtcgccagg gagaatgccg tcgtcgcgca
    40741 tggatgtccc cttgacgcgc aaggcgaagg tttctcctcg tcccagcatg ctcttcggaa
    40801 cctcaatgcg ctcggtttgg gggatgggtt cgatggggtc gccggcggcc acaatgccga
    40861 cgaggggaat gcgagcggac tgtgcgatgg cctgcaaggc tacctccacg acacccggaa
    40921 tcgtgcgtgc cccgccttca tagcgagtca cggccagccg tttgcgtttc aattgatccg
    40981 cgagggcttg ctgagtgaga ccgagatcga gtcggacgcg tttaaattct tgtggggtca
    41041 tgtatccatt ggatacatct aggagggggt ctgtgtcaag cagacagttg tgtcggtgct
    41101 gtcctaatta ggccaacaga aataaaatca ggacactcta ggccaaattg ggtcagtcga
    41161 ggatttaaat ccgacacgca gaagagattc aagttaggac actagttgga tgagaattaa
    41221 aacagccgag ggtgaacaga tgtagttcct cttgtggatc gggtggagca aggcttgggc
    41281 gatccggtac ggaccaggga aggcgccaaa aacgaggcat cgtatagcaa aatctagcca
    41341 cccctcgagc tgagaaggtc agtgcgtatc tgagccctca ttctttgggc gccacaccga
    41401 aacgtagtct aagaaacgcg catgactctc tcgagctgtg gctacggcct ggcgtaaccc
    41461 ttgagtaact ctggtctgaa ggacaaggtc agcggttgct tgcttaattg ccagatcaaa
    41521 gacctttgag gatacgtcgg caagtgcctt gacatgacgt gcgtctaatt ccgcaagctc
    41581 agattgggcc tgcgttattc ctttatcaat gtcaactagt gtccgtgtca gaagcgtatg
    41641 ttgtgatcgc aaattttcta aggctgaacg tgctgcatcc ttggccgggt tcgctataga
    41701 cgtaattgtc gtgacatttc tgcaaagtgc atcacctact gggtcgaagg gaggtcggtc
    41761 tgggcacact tgttttgtaa tcgattgcgg aattgtgtct aacatgctga caatatcagc
    41821 ttcctgttga gcgatccgat tttgcacgcc ggttattgag gcggccgcgt tggcgccctg
    41881 ccgttgaaga ctttgaagtt cagaagtaat gcgttgattt tgttgcgcgt aagcattacc
    41941 agcatattcg agggcatcct tttgagcacg atttgcttca tctagggttt tttgtgccag
    42001 ccccaaagca gcttcggcat tttggagatt ccggttagca agttgaagta tcgtgtctgt
    42061 acgatcagcg tactggagga atgtgtccag gagctctgat actacactct gtttgacgcg
    42121 cattcgattg tacagtgtgc ctaggaagag acaatcccaa tacatactgt tcgccttagt
    42181 gaattgagtt ttttctttct cccagagcca gctgctcttg ttgatgtctt gatcccgatc
    42241 ttgagcagta gggcaacttt ctaggatcat gctccgcaca gtcggtgttg gcccctccgc
    42301 tagaagccgg tagaaagggt tccatgaggc acgtgctgaa atgacttttg cagcggcact
    42361 aattgagatg gggtcagagc cagttgctgc taaggggtcg aagaacgaga gcaagtcagt
    42421 gtccttggga agcttgggta gattgctcgt gcggagcgac gggttattga tgagcattcg
    42481 tagcagcacg cgtgtcgcat ccaaatgcaa agggaagtgc actggatcgg tggccgatac
    42541 cagaagtagt ggagggatct cggaaacacc aggtagcgac aggatagctt taagagtaac
    42601 ccgcgattct gtttcccaag cggccattcg ctttggaggt gccgtgtgtg tggcatcgtt
    42661 aacggcggca aagtctagtg catctggggg gcgcaaggcg caccggccac cttccttacc
    42721 aaatatcttg aacggtgccg gttcctctga gtagcccagg tcatgagtgc agactcgagg
    42781 ccactcgatt gtctgacagt cagatcctat agtcccgtca ggctgtacgg aacagatttt
    42841 ggtattcgcc gcttcagcat caagccagtg aagccatctt cgataggcat tcgcgtcctt
    42901 ctttttcacg gcataaagca tgactcctaa gctcatgtcg ggagaaaatc cgtttgcaca
    42961 tagatcgcga aagtccttgc tcttctgatc agtcccagcc ttaaagcaga agttggacca
    43021 aagccactta cgatgtggcg atcggtacca tcgcccagat ttggcttgcg cttgagcgac
    43081 tgcaacacat ccatcctcga tcccggacgc acacaacagg ccattaaaca gtgtctggtc
    43141 accgtcgtca catgatgccc cagggaaggc ctcaggcagc atgtctttcc gagtcccttt
    43201 agaggggaag ggggtatatg cgttacgtgc gcctgcgcat cgatcacctt tgtttcgcca
    43261 cattgtcaaa gtgtcatcgt caatagcgct gccgatgttg gtgataggcc agataggtag
    43321 gagaggctta ggattggcag cttgagagtg tccgcaagta attatgagca aaatcgtggt
    43381 gtagagtaga ttttttgtag cttttctcaa gatcccccct cagtggcctt acacgagccg
    43441 cccgcatcat aatctggcgt aatagtgaat agatgttgaa tgcaaatcta tgtagacaaa
    43501 agcatgatct ccgtcaagag gctggttagc aacccgacag ccccaaaggt tgaaatatct
    43561 tgttgagagt tggcctcccg catggcgtac agacacgagc gatcaccagt gggatcccat
    43621 gcgattgatc acgacgaagg aactgcgagc gttgcacatc gccacacgac atcatcacct
    43681 cattaacggc cggctggcga tttttcgcta tgcggacgaa tatgggttca atggcgccgc
    43741 ccggcagttc ggactggatc gcaaaacagt gagaacgtgg catcgtcgct gggtcgtcag
    43801 tggaccggct aggttagtgc cgcgtcaggg aatatttggt tggcgtattc accgggcgat
    43861 gagcagtact ggcatgctcg tgccagccgt gggttaggcc cgccatattc gagggagact
    43921 gcccttgtgt gcggggcccc actattcgtc cgacctgaac ttcgacgagt tgacctgggt
    43981 gcacgtgaat gggaaatggg gccgcggcgc ggacaagggc agatttgaaa gcccgcgccc
    44041 atataaatgc ctgttgatat ttttagatgg aagtcccaca atggcgccgc tttaccacca
    44101 cgcaattata ctcatggtga gaaaagacgc agaaacgcga gatgtacaga taaaaatatc
    44161 tggcaccgcc ttgctcaaat cgcgcatgga tgagtttgtg gcgaacaatt cccagacatt
    44221 taattccgtg aacacgctgg tggatcgtgc ggtgcggtag gtggtgaaac gctatgaagc
    44281 gacagaaggt ggaattgata aggatgggtt cccgctcgac gtagcccaga gaatcggctc
    44341 ttctacgaac gcgcccccca ggccgaggag aagaagcgcc atagccccct tgagcgcgcg
    44401 gcatcggtct aggcattgaa cgcaacgcta ccaaagcgac ctacccagac tggtacggga
    44461 atggctggca gacgacacga acgtgaagtg tttcccgccg aaacgttaaa agcgttctcc
    44521 cgagcgtgcg agctgtgcgg tactgttggc ggcccgcagt gtcgattttg catgaggctg
    44581 actgccgagc gtgccgaatg atacgtcggc catgcaggag gccaccgccc cagcggtcag
    44641 ctggccgact acgccggacg gggggatcag agaccaagcg tgtcttgagc agaaaggctg
    44701 gaggctggtc gagaaatgag acgctatgtc ggcatatatt ccgagcatca gcactgatct
    44761 gtttatttcc caagtatgta gcgctcactg tatcaccgtc gtcgacgatc cgtgccgcct
    44821 gaggcgcgtg cctgttcatt ttgtcgagcc gacgaactca gctccatacc tcacaccgta
    44881 gtcttggagg acaccacatg cgcacactac ccaggctagc ggccggtctt tccgtactgc
    44941 tcctcaccgg ctgcgtagac ctatcctttc gcttcacgcc ggagaatctc tgggtgaaac
    45001 cgatggcgga gggcaagacc tgcatataca ctctgtttaa tttgggctac ggcaccaata
    45061 ccgtcgagca ggccatggcc aacgccaccc caccgattca gaagattcgc agcatcagtg
    45121 gaacgatgtt tcatttcata gtcttcgaat cacactgcct gaaggtcgtc ggtgagagcg
    45181 ctccagccgg cgagggaaca cgctaattgg aacataatgt gttcgcaaga ggaggacccc
    45241 acatgcgcac gttctgtacg ctgaccgctt gtctcgccgt gcttctgctc atgggctgcg
    45301 tcagcatcac caatcgcatc accccggagg atctttcggt gaaaccgatg gcggagggca
    45361 agacctgcat gtacaccctg tttgagttgg gctacggcac caataccgtc gagcaggcca
    45421 tggccaacgc cacgccgccg attcaaaaaa tcagaagtgt gagcacaagc ctcttcttct
    45481 tgctgtttgt gaataagagc tgcctcatcg tagtcggcga gggcgcacca gccgccgcgc
    45541 catagtggcg acgccaacgg agacggtctg tcgacgatta ccgcgtactg gagggactat
    45601 acaatgcatc gactgatctg ctgcacctgc tggctgacgg ccctactcct ctccggctgc
    45661 ataaatgtct caaatcgact gaccccggag gatctctcgg tgaaaccgat ggccgtgggc
    45721 aaggactgca tgtacgccgc gtttgggttt ggctacggca gcacgaccgt tgagcaggcc
    45781 atggccaacg ccactccgcc gattcagaac atccgaagcg tgggcgtaag cctcttctat
    45841 ttactaggtg tagccaggga gtgcctgatc gtggtcggcg aaggtgcccc gggcaccacc
    45901 aaataagcgc accgtgaccc cacacgaccg atggtgtgcg agcgcgagct cactgcacca
    45961 gcctggtgca tggtggtgga tctggagggt ctgacgtatc gggaggaggc ggtggtggcg
    46021 gaaccggcgg ccacttgacc atacgcacgg gcgcctggca tctcatgcgg tgaccgcgcg
    46081 atatcgagcg tagatatgct cggccaagtc agccagtcac gctggaatgt acggcgcctc
    46141 aagcgtggcc gcctccgatg tctgcgcccc acgggcgtag cagtgtggag aaggtctccc
    46201 ccgcatcggc ctcctaggta tggtgccaga gatgttgata gccctgtgcg atctggtcag
    46261 acttcacgcg tgcagttttt ccgacaaggt ggatgatgtg aacataggcg gaggtgcagt
    46321 taatggaaag gatattgttg ctagaatatg aacatacgga catgcaagta gatcctatat
    46381 gccctgggta ccaagagtcc caggaggcca gtggataacg agtgacacgt gctatcggct
    46441 gtttcctccc gaaaaaaata acttgtttca tacctaaact cgccaagcat gccgcccgat
    46501 aaaagccttc tttcccctgc cctcctcgac gaggaccgtt gggatgtcga tcatcctcaa
    46561 tgcggcagtg ggtcagtcag agcaattcta gccgaacccc gcaggtgctg taaggtactc
    46621 agtacgatag gagcgatctt gttttctgga cgtgtcgcaa gtctcccatt ggggcattgc
    46681 ctgtgcgggt ctctaggtct cctaagcggt tattccttcc actgcaggtg gttcagcaga
    46741 tccgcgagcc gcactgcggc tttggtgagc tggatatccg catccacctt gtgctcttgc
    46801 agatacgcac tcttcgtcgg gggagtcacc actcgactct gcacgtgtac gggtgggtgg
    46861 ggccgtttgg agtcgagaat cacacagccg gagtcgagtt cagcgggaaa gattcccgcc
    46921 gcggccacat gcacggaatc ggtggcccat tgttccggcc atcggtgata gtcgccccgc
    46981 atccgaaagg cctgagaggc ggggtcctcc atgcgcgcct tcagatggtc cgcgactctc
    47041 gccaccgttt tgtcggtggt ctggctcacg cagcctttgg ctccccccac gacatccacc
    47101 aggcagtcat cccaaatggc gtgcaggttt tcatgcgtcg gtttcgttgg gcaggtgggg
    47161 tcagcgagga acagcaacac atttccgcct cgatcgttct tgccgtgctg cttagtgacc
    47221 tcgctggggt ccgtcagcat cgtgggatgg gagagcgtgt cggccgccgt tgagtaatag
    47281 ccagaggcta catgcagggg ctgatggata tcttcgctca ggtgcagcag ccaacgaagg
    47341 gcttgaagct tcgtgaaggt ggctgagtcg gtgcggcctt cgagaatgtc cacactctga
    47401 tggatcatgt gcaccacgtc cgttgtattg gtaaacggca gggcgggatc cgcgggatcc
    47461 ggatgcgcca ggtctgggta gtgcgcaacc cccggcggca gatctacgaa gtgccaattg
    47521 gtgttgtccg gatgggtggc gacgaactgc ttggcttcct ccatctcagc tggtgaaaat
    47581 cccggaatcg tggtatgcgg gttcttcgtc aacgcacgaa tctggtccgg ccagacggag
    47641 agacgcgcca gagtactcgg tggaaggtcg tttcccgtcc ctacaatctt tgcgatagat
    47701 tttgccgtcg atgcggtgag gtgtccttgt acagcctcgg caatccctct gtggccttct
    47761 tctccccagg caccgacctc tgaagtcgtc acaatcacgt acaagagtgc gaagagaatt
    47821 atcatggctg ttcctcacga tgaataaggg gaatcatggg ttatgtcctg cgatctactg
    47881 aacggtgaat gaagcataga tcatgccgtt tctgcgcgaa tcaacgcgtt agaaattcag
    47941 cgtcttatga aaaacgatga gtggcgagtg tatctcaacc gatacaatgg tgctgagcca
    48001 tctcagcttg gagaggtatc cgatcgctga gcaggaaatg ccgccatcta gcattgaaga
    48061 ctagatggcg gtccaggtga gtcggaaata aatgttcgag gggcgttatg cgaacgaggg
    48121 atcgcgaggt tattttttcc cgagaatggc atccttcgcg gctttcgcga ttcggaactt
    48181 cacgacacgc ttggcaggaa tcttgatggc gtcgcccgtc tggggattgc gccccattct
    48241 ggccttccga ttcttgagaa ccagcatgcc taagcccggt agcttaaacc cgttcttact
    48301 ttctttgtag gccagcgcgg ccaagtcatc gagaatctgc gtggcggctt tttttgtcag
    48361 gtgagatttc tccgcgaggt actccgcaat tccgtccttc gtcatcgctt ttgccatgct
    48421 gttatcctct cagttatgga agtttggctt ttctactgcg tggccgaaaa caagtcaaga
    48481 agtaacgatt ttggtattgg aataggtgtt catcctccat aggattcgag acaggctttt
    48541 gtgcgactgt gcgagagggc ctaattcggc ggacaaacca tagtcccgta tgtcgatact
    48601 ttcgtttgct gagtactatt catcttgaac catttccgga aagctcgcca tgaacgaatt
    48661 ccctaggaga aaagggattg cttttcgatc agagggagtc tgaacctcaa cggctctcca
    48721 ttcctcggtc aaatccccag tcattttctt ctgaagaatc acaacgaaac gtcgctttct
    48781 tgttatgaca gcaatcccga cggttggact tctctcattc atcacaagaa actcgaccgt
    48841 tccaccaccg ttaatagccg tgcagaatgc acgccccaga gtgctggtgt ctggatgcga
    48901 agaattcacc ggttcgtcac gaaggtgtga cggaaaatgt tgcttgtgca gtttattctc
    48961 acaatcgttg tcgaatttgt tcagtttgga aatgcgtgcg atttgttttt tgatttctgt
    49021 cgtcttgtcg gttaggttgc cctgaagtct ggagacctca aattcgtccc ccaactcttg
    49081 tttatacttt tgcagttgat cagctagggc ctgcagtttc tctaaatctg ctgcgttgtc
    49141 tttcattgtg taaatagaca ggccaatatg agcaaggtct ttgcagatgc tgtaagcacg
    49201 caccttcgcg tgttgctctt ctacgagtga ttcccggctg gggtatgaaa tcgttgctgt
    49261 ggcatccagc catctcgtac taatgtcgga tggaaatgct gaaaatggag ccgcttgctc
    49321 tactgcgccc ttagcggaga ggtcgtacgg atcgaagatt ttattggtag ggcttgacgt
    49381 cctctctagc ctcacatcag ataattcgcc tcgtgcatga actctgaagc taataaccac
    49441 ctgtttgtct ttcccatcgt acgaacgcct ggtcaaggca ggagatgcag caatctttgc
    49501 tgtcatgtcg ttgaggtatc gaagtactgc agagttggcc gcatcactcg gttcgtctgg
    49561 cgcaatggca acgctagata tgggcttcag cttctgaact aggtcggtcg ttatcgattg
    49621 gacacagcta tcgttggggg caatagtgtc ccctcgctcg cttgtctcgg tgacatgtgt
    49681 ggtagttgag tcgttggcct cgacagcaat gggcccttga agaagggcgg caccaaccat
    49741 cagagtgaga aacagcacaa acgcctggat gttaagtttc atggcttttc caatgcacaa
    49801 tgactgaggt tggtggcggg tcactagctc tccgaggctc accgtgccgc aacgaatgtt
    49861 gacgtctgcg ctactgtttg taaagtgaga tttcgtcttc cgcccgattg gcgccctcgt
    49921 agttacaagg aaggtcaatc gagacgtttg atgtttccca tgccttgtct tgaattgagg
    49981 ttcgcgcgtc atggttctgc ttctttgtcc aataaccctg caagccccaa ccaagtagat
    50041 atcgcgaagg aagagcttct ggtctcagac cagggctgat acacggcaaa cagtaggacg
    50101 tttagctact tggcggcatg agagtttgct gtcagaatct ctgctcgact ccaaacactg
    50161 tgcctgtacc acgatgctct tcaagccatt ttgagataaa tgcgcgacct ttattgcggt
    50221 gcgtaacatg cgcgtcgatt ctgcgacagc cccagctgtg cactggttga tcaaattgga
    50281 tagaacatga agaaatacat cgctgtcttt gatgaaaccg gaaccagcaa tcgcccacag
    50341 tcaaccgagg aatctggctt tggagtggga gcagttctgt ttccattgga acaggctcca
    50401 gaattagccc ggatttcgaa acacctcggc tcaattgttg gtaaagagga ttataaatac
    50461 aaggatgtgc ttcaaaacga ggcagccagg gagctgttca tccagacgct gaatcgtacg
    50521 agtctctcca tacatctgtt cgcgttctat gctcatggcg cctgcatgat tcatgagggc
    50581 agacgaacaa aagacgcggc cttggcttat gggagggagt ataaagaacg gtcctctcca
    50641 agaggcgaac gatttgttcc cttcgattcg ttcatcgcct acatggcctc ctgtatcgca
    50701 gcccatgcgg cgacgaatga gtatgccgta gacatctatt gggatcgtcg cacagatctg
    50761 gatcaaatca aggcatcgtt tgatgaacac atcgcccgcc aatctgacac ccaaagatat
    50821 cgaaatgtgg cggagcttgt gtcgtttaag gggcgcgcac aaaatgactt ggcgttgatt
    50881 acacgtctgg ccggggtcct tgccggcgat gtttggagat actttgagtc acacggcccc
    50941 aagatatgga cggccctcga cgttacaggt ctgcctgggg aatatgatcc gcacatgacc
    51001 attgatgatc ccgacgtgac agggcctcct tgtgttgccg tcttcatgga acgactcatt
    51061 aaggtagagc ccgtgcggac ctcggccacg agtatgttgc aggggtatta caagcgattc
    51121 ctaaaaaatg agaacaagga gaacctcatt tcgttcgggg atcctcatgg acgcctggga
    51181 atcatcggga tagtcaatgg ccaccactgg aaggtctatc aattgccaga ctaagcacgc
    51241 ggttcttttc gcgtggcacg tgcagtgctc ataaaacgtg ataggataga ctatcacgaa
    51301 ctgagagtag tgagcacagt atcgatcgag tgatatgaaa gtgcgaagga cgagcgaaaa
    51361 gggtaacagt acgagccgcg gcaacgatgc tggtctttgg ggaggcccgg catggacatc
    51421 gacggttgag tctgtggtcc gttcttgtgt gtccccgcat tcaaagcgcg cgtatagcgc
    51481 gtctctccgt gcctatcgga tgtggcttca gcagacccca cagcctgaat tttcgcgctt
    51541 gtctatcctg agatatcgtg acgcgctcct acatcgcaaa cacctggctc cgaaatccat
    51601 caatcttcag ttaaccgccc tgcgtaagct tgcgaaggaa gcggtcgacg aaggcttgat
    51661 tgatgatcga acagcggccg gcattctccg cattccgatg gtgcccaatc gtggggtgag
    51721 ggctggtgtg tggttgccgc gtgaacaggc gcagcggatc ttgagcgggc caaacgtgcg
    51781 cacgatcgtg ggcaaacggg attatctcgt gttgatgctg atcctgcggt gcgggcttcg
    51841 gcgggcggaa attgcgcagg tggcggtgac ggtggtgcgc gaacacgagg gccgatgggt
    51901 actcgcgaat ctgcgaggca aagggaaacg gcttcgcacc cttcccctcc ccggtgtggt
    51961 gaagaatgcc tgggaccggt gggtcgaggc aaccgggatt gagtccggcc gagtcattcg
    52021 agccatcaat cggcatggcc gcgtctgggg cgagggagtg acggaggata cgatctggcg
    52081 cattgtcgga cggtacgcac agctggcgtt gggaaaacac gtggcgccgc atgacttgcg
    52141 tcggaccttt ggccgcctgt gttatgcact tagcaaagat atgcgggaga tccagcagct
    52201 ttatggccac agttccgtga tgacgactga gaagtatcta ggcgtagaac ctcatctcgt
    52261 ggattcggtg acagatcggt tgggcctatc gtgagatgtt cggaatacag cgaggcctga
    52321 caatcaccgc acaatcttgt ttgtctagct ctgccggtgg ttcgcaataa tgtcttttgt
    52381 cttcgaatgt ttccgtctat gcaggaaatc caagtgggca ttgaggcttt gtcttcagag
    52441 aagtcgaggc gttcttccca aatgacgaaa atcgaattgc ttgaacagac actttgcagc
    52501 cgtatcacac tccatcataa tcttacttac atttggtagt atttccgtgt gaatctttgc
    52561 acttcggatg atgattagga ggatggggag aatgaggggg atgcgatagg tgtccaccct
    52621 ccagattctt gcccgccact gattcgttgg aagcacctcg tttccccggt ggctttgaac
    52681 cgggaccagg tcttggaggt ttaggagggt gcggaggaag gagggcaaag ccgtcaggtg
    52741 acgaaataaa aatcaaaagg attgttgcag ctactatcgc tatgcctagc atccttcttc
    52801 ttacgtaagg catcctgacc ccctagacac ttattcagtg tgatccttga ctccgataga
    52861 tcttaggccg agagtcgtgg ttcatgtagc ttcgttggtg tcagagagcg tgcgacccgt
    52921 acgtctgaac actcccacca cctctcgatc tccgggaagg gggatcttcc cttcgagcgc
    52981 gataaggcga tcatagttat catcttcaat caatcccccg tgtcttatcc ctcctccggt
    53041 gttgatggca aacatgagta tctgcttaag caataagaaa tcagcagtca cttcatctga
    53101 catcaacctt ttaacacctt cctaaccggc ccttctcagg gatccaaggc cctgctgccg
    53161 aatcgtggga tttgtggttt cgattcgaat ggcggcttgt ccgaatcgca atctcaatgc
    53221 aatttcagtg acacggccac atgtctctga ggcgctggtg aactgcgtgc actgctattc
    53281 tatccgtgat cccgaccttt accacatagc ctggatagtt cggatggtct tcgagcaatg
    53341 tacgccagag aaatgtcgtg gtgaaggcat gagatcagat ctcactaagc ttgcggtagc
    53401 ggatctcaac aaacaatgca aattacttat actcttctgg aagagtatgt tcaatgacac
    53461 gggcaatact tgagaagggt tttgccgatc aataatacgt gtatttcaaa tatggtttgt
    53521 aaggaaatat cgtttgcaat gtatacgtag tgagagcatc atccgcctac tttataacgg
    53581 aattggcgac acccttcttc caacaacgcc gaaaggcaat agcgatgcag ggggaaacgt
    53641 tctagcctgt acctatggca accaatactg aaaagttaac gccgttgaat attgcgcttc
    53701 ggccggccga gggcagaggc ggtctcgagt ggctcggcca gcgaatcgct ccgcagcttg
    53761 cttttcggcg agccgatgga aaacattttg gtgatcaagc tccgttcctt accaaagtcg
    53821 cgcacagccc tgcccgagta acaaaaatgt cacgttcatt ggcattcaca gggattgcgc
    53881 cgtgatgact tgtctcgtca cggataactc gaaggggaca gcaagatcat tacagccttc
    53941 gcctctttgt gctacagggg cgatgacttc ccagccgagt ttgaggcgag attggctgtg
    54001 gagtaaccag gtgtccacgg aattggaaga gggttaggat gcgaaggctt ttgcagaaac
    54061 gctcgcatgt ttgatgtgtg gccaataagg atccccgtgg caaagctggt tgatggggcg
    54121 tgtataagaa tgtgtgattc attagaccaa gaaatgcttt taacatggag gtgttcatgg
    54181 tctgcaaatc gaacgcgttc aacgggactc tcttaaaaaa taagaaatgg atgaatttta
    54241 gtgaaaatgt gacctttagt gcagaggaat attttgaacc tcagcacaca tctcctgggg
    54301 gacccccaga tggactgcaa caacttgtac atgtcgttgc tcgggcaact aacgaacaaa
    54361 aggcgcttca cgcaattggc tcgggctggg cgtacgaaag cttagctcag tcagattcgt
    54421 gggttgtcaa tttgaaaaac ttgaatcgcc gcctccataa cgtaataccc actgctctca
    54481 ctgatggctg gaaggaaaag cagggcaatg ctgcggccac tacccatctg gttcatgttg
    54541 aagcaggaat gaagatcatt gatctaatcg aaatgctgga aggtgttggc ttagcaatgc
    54601 cgacattagg cggggcgaac ggtcagaatc tcgcaggcgc tatttccaca tccacgcatg
    54661 gaggagattg gaaccagccg ccactacccg atgttgttcg agggattcat ttagtcaccg
    54721 atgggggccg tgagttgtgg attgaacggg catctgaacc gataacaaca gatgttcgct
    54781 tgaagccagt gttgacctgt ccagatacgg aaattattcg taacgattct atttttgacg
    54841 ctgcgttggt ttcatttggg cggattggcg taatttacgc atttgttctt gaggtaagga
    54901 aagaattccg cgtagtggaa gtggcaacca ctgcagatga tgacgaatta ggggaactgc
    54961 tcctagcgct gatggctggc atgaccagtc cgaacgatcc ctttgaaaaa ctgttcagtc
    55021 gactcagggc ggctcctctt cctcctggcc tagctgaaac tgtggaactt gctcggccgg
    55081 agaataaacc ttatttcttt cagttcgtct ggagctcagc tgctccttcc tccctcaatc
    55141 ctttccatgc cccgcaagat acaacttatg tccaacggcg ttggataaca acggttggga
    55201 ctaatctcaa cccaccgata gatgatttta tggcagacat gacgaaaggg ctccttagtg
    55261 tcattcctga cggtctcgct actgtttcag gtcaggcttt tgtggcgagc ggagggttaa
    55321 agcatttggc gacgaaagcc ctggaggaga gatttaaaaa ggccgtcaca gaaggtttaa
    55381 gggcatccca tcatctctta acgagtggaa caaagagagc tagcgaagat atcccataca
    55441 gtggtgacta cattgaagtc atcttcgatg cacgggacga caggatcatt ccatttctcg
    55501 ggaccatccg tgagaatagg agcgcgcatg acacgctcgg ctggatttca ttaaggccct
    55561 cgcgcgcttc gcgtgcatcc ctttcaatgc acaatgtggc tacctcttac gccatttcca
    55621 ttgaggtcgc gcttctcaag gggccctcgg gtaacagggg gttcatggat ttggtgcacg
    55681 aggcggcgct taaatatggt ggtagacccc attggggcca aaagaacaat ctcaatttcc
    55741 aacaggtggt ctctttatac ggatcgcagc ttcaaaattg gcgtacatcc cttacacaag
    55801 tcagtgcttc ctcgacatta ttcagtaaca acttcacacg gcaacgaggt cttgagccga
    55861 caaatactga gcggaaggtt accgccttgt ctgcgagcaa aaatgagctg attgtcgttg
    55921 ggcttcggaa aggcaatgac aagtataatc atgaagtgca attctcccac tgggcctccc
    55981 ctgcagtggc tgaattgcaa ccattgtcac ctcggcatcc ggttcaattc gatcacgggc
    56041 gacgttcccc tagtcaagaa gataggggat gggttggctg gcacccgttg gaacccgctt
    56101 ccggtttgcc agataacttt atcggttccg ctgtggaagg gggcaaagct tacgtgttct
    56161 ggattgcctc ggacggttgg gtgaactata gagtgcgcgg gcaggacgga cactgggcgc
    56221 gctattggtt acccgttggc aataccaatg cccgcccatg gaatggcgtt ccgggcggtg
    56281 cagttcatgc ggtctcgtgt caaccaggga cgctgcatgt attctatacc gaccggcagg
    56341 gcgggatttt ggcagtccgc ggaaacacaa cgagtgcccc agggtggcct catagccagt
    56401 ggatcaaggg ctgcaagacg atcgcgggtg ggcacgtcac tgctgtgagt cgttcccaag
    56461 ggctagtcga cgcattcgtg gtgggtacag acggacgggt ctatacggcg gcttggaacg
    56521 cgcaagacaa ttggagtaac tggagagttg ttaaagaagg actaatcgct aagcctggat
    56581 catatatcag tgcggtctct cgaaacgtta accttctaga tatttttgtc gccgacgaag
    56641 ggggaaagac cctgagcgcc gcttgggcac ccccatcggg ctggaatggg tggtggcaca
    56701 ttcaaggagg gatttcccgt cctggaggtt atgttactgg catcagtcgt tcaagtgata
    56761 agttggacat ttttacgaca gaccctgatc aacggattct caccgccgcc tgggacccgg
    56821 gggtgggctg ggctggatgg tggcctatag gaaatgcgaa ggctcaatct cttgtgtggc
    56881 cggtatcacg ctcgcaagat aaaattgacc tcttttttgt ggatcccgat ggatctgtgc
    56941 agactagcgc atgggccccc gggcaccctt gggacggccc gtggagcatc agttaaagga
    57001 tgagagatga agtgacgccg agcgagtttg tcgcacaacg ttagggagaa ccgaccctcg
    57061 aagaagtcgt ctgctctggg caggaaccgt ctcagaacag ggccaatggc aatggctcct
    57121 atgatgttga ccgatccaaa acaaagtctt cctgaagtat aggcatgact attcattctg
    57181 taccaacgcc ggaaatctta tatccatgaa ggaatatccc aacatgttaa taataggttt
    57241 ccgatcagaa ggtgtttgaa tctcaattgc tgtccactct tcactcaaat ctccctgcat
    57301 ctttttctgc agcacgacaa caaattgtcg tttcttggtg gtgacggcaa tgccgaccat
    57361 aggtggattc ttgtcaatta ttttgaactc aacctttccg cctccgttga tggctgtaca
    57421 aaacgctctt cccaacgtct ttgtctccgg ccgcgatgat gcgacgggct cctcgcgaag
    57481 atcataggga aatccggctt tcaatatctc tgatttgcat acatgatcga atcgatccat
    57541 ttcagaatcg gtggcgagct taagtgtgat ttcttcagat cgagctgcaa tcctatggcg
    57601 tatgtctttg ataagtccag ggacatctga gtcgtcgaag gactcgagat taatgtcggg
    57661 aaagcgcaaa ccgtttgcaa cgccttgtag cttttttagt cctgtttgat tattcggcac
    57721 ttcgtcgatt atctgcggca acgttctcaa gtgtgtacga catgcactat attgatactt
    57781 ctgcctcccc caggattcct cagacgcgga ctgattgatt actaccaact cagtgccagc
    57841 gagccccctc ctacgaacaa ccgggccacg agagcggtct gatgtggcgt ttaaattctc
    57901 tactgaaaat gtatatacga catccaaata gcgatctgcg atgtaccgcg gaaactgcgg
    57961 caagggaaca gcgctcttca acgcttgacg ggccgcgaga tcgctatact cgttgccgga
    58021 ggactgttga atgttgacat tagtgatcat gcccgcccgg tctaatctga aatttacgac
    58081 agtcgtcagg actgcctcgg gaagattcct tggaagaaga gaccagaacc gacttatttg
    58141 agtttgaatt tgtgataagt attccctcag tcctccaggt tgctctccat ttattgcatt
    58201 ggtatccccc aatggcgtga gcgatgcgga tgttgacgat ctcgaggtgc ctgacagcca
    58261 aagattgcaa gggttagtct cctgcgaaga gagctcatcg gacagattaa tttcgtcggc
    58321 gaacgcgact ggagaggaac tcaaaatctg tgtgtaggcc aatgacaaag aaatccagaa
    58381 aatagccagt gcggtaatgc tcgtcaacct gttcctcaga taattaatgg aatatgtctc
    58441 tcccatcttc acacccccct gcctttgctg tctgagtaca cacctttgcc ttttgagcta
    58501 acggctccat cttgccagac agcatatcgg caattttcat tcgctgatat ttcgtaaaat
    58561 cagatttgta gtcagtcact ttatagtcat catagttgta atggacggca aaggtgaatg
    58621 attcataccc cctctcgcct accttagcat gtagaagcgc ccgacatttg tagtcagggt
    58681 tattcctact gacatcctct gggtaagtga ccctcgtctg aatcagtggc gtggttcctt
    58741 ccggccatcc gttcttgacc aactccttga gtgtgatctt ttgtagatcg cttctagctg
    58801 cggcattccc acagctatat ccatcaacca ccggaccgag aagggagcag ccaataacct
    58861 gagtgagaac aagcacggcg ccgcaaatac tgtgcatgca tttcgacatt cttttcctta
    58921 caaaggccaa tgttgagttt agccattctt ttccaatggc gctccatggt agctgcttcc
    58981 caatcagttg ggaacagctt ggagggcagt gttcgtttgt ggagatatcc atagatcgtt
    59041 tttctatcaa ggcacgaatg gtgtcggttc tcagagattt actatttatc agtcttgagg
    59101 tctgccgcat agaattagaa cataggctta gtgcatgctc atcatttggc tctcaatatg
    59161 cgcgaagcct tccatgatat tttctcctag agaattcact cggcgttcag cattgacttg
    59221 agctaattct cccctcagag ctcggctttc cattgcgaaa tctgctttag ttatttcatc
    59281 ttttttatag ctactctcaa gttctgccac ccgtgacaac acatcgtact ccgcgagcaa
    59341 gctatccatg tgaatgccgt tataatttat gtggtgctgg atctgccgtg ccaatgcgcg
    59401 caaatgtagt aaatgttgcc tctcgcttag agtgctcgaa ggtccctttg actccaccga
    59461 ttcgatttgc agatctaatt tgtgtaggtg gttcttgaaa tccctgcgaa cgcgcgcaag
    59521 ttcgccatac attgccatcg cagcatcttt tcttttcgtg atcgatctca tctcagccag
    59581 cttggccaaa aatgcgtctc gaagaccacc attaatgtcc gcttccccaa acggaacaga
    59641 ggccgagttg ctttcctggt tttgagccca tataggatgt gggaagatgc ttgcccaaac
    59701 caagactaac cagagcgcaa tgcgcaatcc atcccaccgc tccgccatgt tgacctcctc
    59761 tggtactgtc gtgaagtagc ctagcgcgtc ggtaacgtag aggctggcac tttattcctg
    59821 aactggattg tcttcaagaa agatcctgca aaaccatctc tagaagtgtt tactaagcga
    59881 tccgaacaca aaacactctg tcagggattc actgtaattt tcttagacta actcggttac
    59941 aattctttgt ttagaggggc gccccctcga tcgactcgat atctgcttct ctagcatcaa
    60001 tttttgaccg aagaatgacc ttcccccaat tccgtggaca cctggttaag ccactgccat
    60061 cctcgcttca aactcagccg gggaatgata gccgagggcc gagtgacggc gtttccggtt
    60121 atagaacact tcgatatact cgaagatctc ccgcctggct tcgtccctgg tggcgtagtg
    60181 ctgatgatac acgagctcgc gcttgagcgt cccgaagaag ctttcgacac aggcattatc
    60241 ccagcagttg cctcgacggc tcatgctggg cgtgatgccg tgcgcagtga gcagttgctg
    60301 gtacctcgtg gcggcatatt gactgccgcg gtccgagtga tgcaggagcc ccgccgtggg
    60361 tgtccggttg gccagcgcca tggtgagagc gtgttcggcc aggtccagag tcaggcgata
    60421 gcccatcgcc cagccaatca ccgcacgtga gtagagatca aggagcaccg caagatacag
    60481 ccagccctcg tcggtccaga catacgtgat atccccggcc cagactcgat tgggctgcgc
    60541 cacggcgaac tgacgattga gtgtattctc tgccacgggc aaccggtgat tcgactgggt
    60601 cgtcgcacgc cacttcttca cggtcttggc ccggaggccc gcctggcgca tgagccgggc
    60661 cacgcgatgt ttgccgacgt catgcccctg ctggctcaac gcattccaaa tgctggggct
    60721 cccgtaggtc tcgcgggacg ctcggtgaat cactcggatg gtggcaagca gtgtccggtt
    60781 ctgcctggcc cggcggctct ccggtcgatc cctccaggcg tagtagccgg caggtgagac
    60841 attcagcgcc cggcacatca ggcggattga atagcgacgg tcgtactcct ggatcgcccg
    60901 gtatctcatt gcgattcctt cgcgaagaac gccgccgcac gtcttaaaaa atcccgttcc
    60961 tgagtcaccc gcaccaactc ccgcttgagg tacgtgagtt cctcgccgtc ggtgcgttgg
    61021 atggctcggg tggtgccctg agcctcggcc tgccggtgct gggtgcgcca gcgatacagc
    61081 aggctctctg caatccctaa gtcccgtgcc acctgagcca cgggacgcgc ggagtcccgc
    61141 accaaccgca cggcctcctg cttaaagtcc tcggtatacc gacgtcgtgt gactcctgcc
    61201 atgctgccct ccgatttcat cattctcctc cttatggagg tgtctgtaaa atcgggggag
    61261 ggtcagaagc tccttatcac tccgcgtttg cacttaaacg ccgctgaatc aagcctttca
    61321 cgaacttcga gtcgggggaa aaatggagtt tcagagttca tgacttaact tagtttagga
    61381 agacattctt catgctgggt taggagagct tgtctggttt gaattacggg catcgtcttc
    61441 tggcttctct tggtgtccgt gccaacactg agtctcaagt tagcgttgag cgataatagt
    61501 agctgtattt atgcagggag ccgggcatac tgttctgacc aagcaaggtc ttagtgcata
    61561 gaactgcttc cgaaagcatc ccatgccaca tgtacggaca agtgctaaag gaggaaggaa
    61621 aacccagact gatcatgccc catattcctg ttcctccgac ctgcagcttg taaagagaca
    61681 gtggccgcga gtaacgcggc tactcagcca agagcatgag acgctaccgt ccctcccccg
    61741 gtgggaccct ctccatcaac acaccgattt actgagtcct cttagcgctg ataacgatga
    61801 agtcctgcac acacgtgcat tggcgtactt acttgatcca gtaagacccc atggatttgg
    61861 tatacatctc ttgaggggaa ttattcgccg attaagagac gtgtctaacg ttcgcgagag
    61921 acagctcaat gggatactac ggctactagg ccgcacgaag agagagcgtg aaattgtggt
    61981 tatccccgag tatcgccatt tagtgaaaag ggcgaagaag cagacatatc cccgaacaga
    62041 tatttggata gaaatccacg ctggcaaatc gtcgagactg atagtgatcg agaataaaat
    62101 cgaggcacga gaatctgacc tacaacttac ttcgtatgag gcgctcgcga aggagtggcg
    62161 caagaagcac ccaagcggaa gaccgcttct aatatttctt acgccggatg gaaaaaagcc
    62221 gcgatcaggg actgcgggaa agtgggtcgc acttccctat gctgagttag ccgggatgct
    62281 cagaaggact tggttggcac acaaaagtgc accgggtgcc gaatggttac gcctttatct
    62341 tgcgagcatt ttgcgtcaaa actctcgatc tgcttccaga agggcgagtc aaattgaatt
    62401 tcttaagtct tatttgggcc aaaaacgatg acaaagaaaa ttgtgcagcc tgccctgagc
    62461 aaggagcttt ccttctatct cgatcatgcg agcgaagcgg acgatcttta tgagaataag
    62521 ggcattctgt caaaagcgaa actcgacccc agatttgaaa ccgctttcga gaatgtttat
    62581 gagaaggcat acaaagatct cagacacctg attacgcatt tatccaatca cgtttccgca
    62641 cgtgtgaaaa ggtatgcttg cattgaatgg aagcatagaa atgtggagaa ggagtggggc
    62701 tgctggggat acgcacgatc gaaaggacgg cgggcggctc accactggac gggaatttat
    62761 cttgatcata gcaaagggct ggctcggatg gtccttatcc ttagtccatc tggaggaagg
    62821 ggcggaagac gcttgtttgt cgatcgattc aggccaatct ggagaaaaaa aagagagcgt
    62881 ttagtgttga ccgaagatca tcccaaggac tggccagaat gggaagaaac cccttgcatc
    62941 gtttattacg ttcatgaact gcacccgcgt agttctctaa acgaaacctg ttcagcaact
    63001 gagaaagtgg ttgagcagtt ttttcgactc gcaatgccca aactgaataa aaagaactga
    63061 ccagatgggt gggatcgggt ctgttcctca aaaagaggca gcccctgaga gggggctccg
    63121 gaaattcgga cagtgtggta agtggtagcc tggcttcacc gaagccacca ggtggtggcg
    63181 aaccagagga gcgaggcgat gaagagaaca cgacggaatc acggagcgac atttaaggcc
    63241 caagtggcct tggcggcggt caaaggcgac aagacgctgg ccgaattggc ggagcacttt
    63301 caggtccatc ccacgcagat caccgaatgg aagcaacaat tgctggcgcg ggccgcggat
    63361 gtctttgggg ggaccacgcc gacggccgac acgccagatc tcaaaacgct ccacgccaaa
    63421 attggtcaac tggccctgga gaatgatttt ttagccgggg cgctcaccaa ggcgggcttg
    63481 cggagcgcaa agccatgatt gatcgcaccc atctactgcc tgtgcggcga caatgccagc
    63541 tgctgaagct ggcccgctcg accgcctatt accacgcgac gcccgtatca gagacggcgc
    63601 tcgcgctgat gcggcggatc gacgagctgc atctggccca tccgtttgcc ggcgcccgca
    63661 tgctgcgcga tctcttgcgg caagagggcc agaccatcgg gcggcggcat gtggcgaccc
    63721 tgatgcgccg catgggtatt gaggccctgt atcggaagcc gtctctcagc cggcggcatc
    63781 cggcccatca ggtgtacccc tatctcttgc gcgaccttac gatctcgcgg cccaatcagg
    63841 tctgggccgc cgatattacc tatatcccga tggcgcgggg ctttgtgtac ttatttgcgg
    63901 ttcttgactg ggccagtcgc cgggtgttgg cgtggcggct ctccaacacg ctgacgaccg
    63961 atttctgtct cgatgcggtg caggacgccg tgggccgcta tggtccaccg gagatcttca
    64021 ataccgatca agggtgccaa tttacgagcc aggagttcac ggggcttctg acacaccacg
    64081 gcatccagat cagcatggac ggaaaagggt gctggcgaga caacgtattc gtggaacgcc
    64141 tctggcgaag catcaaatac gaggaggtgt atttgcacgc ctacgagact attggggcgg
    64201 cgcaccaggg cttggcgcag tatctgacgt tctataacca gaccagaccg catcaggcac
    64261 ttgacggcca gacgcctgac caggtgtact cggacaatct gacgacacgg ctgacggccg
    64321 cgtagtcagc aacccgccag gcaccactta aggaatggcc taggctgtcc aaccaaccgg
    64381 agccacctct atgagacccg ataactatca tcggataatg caaagtcagt gggtcctcca
    64441 aacgagttgc taataaccgt gatcagcggc ggagtgacag tgggcgccat attggcagaa
    64501 tacgtattgc ctcccaaggt tactgaggcc ttcgtaataa gataattacc ggcttgcgtt
    64561 gaaggggttc cgtcaggagt gacggaatca aaggtgtaag aaccttggaa cgtgttggac
    64621 gtactgaacg gtggactcag aaacggcgaa acagcgagga cattgccttc aaactgaaag
    64681 gtgattaaag cggcgtgact gccccctatc agtccacccc accctaggca tgctgcaagt
    64741 atagctccga taccgacacg atactgacgc acacaacaat tcagcattct accgctcctt
    64801 ctcattggtc ttggacgata cgccaagaat gcactacgac aattctcgcg ccaggatagt
    64861 agtacactac tcttattatg gcgacgcata aacacttaca tcttatcgcg ctgatggatg
    64921 cagtgcgtca cgaatgccag agtgaatgat ggcacgttca cctactaatg ctcgagacaa
    64981 atccggagcg atttcacagg gcacgggata caattataaa atgcatttag catcccaatt
    65041 aatacacgta tttaatcgta caaatttact tgacgatcat tcacctgaaa cagtatcctc
    65101 acccgctttt caagacatca cgccggccaa attcaccatc cccgaaaggg ctcccagcgg
    65161 tggacaccaa tgatcccaga ctccgattac agacaaatgt catgtagatg gtagcgctgt
    65221 agcaagcagt gcgaagctaa caatcgtgcc aatcgtgact aacaatggcc agccattttt
    65281 ttacaccaaa ggtaagatca tgaatgtggt gtttttggtt ctcgggatat tcctactctt
    65341 cggctgtgtc cctaaagaca tgcttgctaa gaatgaaggt gagcgtccaa ttttatgtaa
    65401 ggagtttaaa gcgactgagg cagatgggct tagcgatacc tatgttgcaa atcgcagttt
    65461 ctggaggacc ttcaatgcac actattccag aaatgattcg ggttctaact caaatggtcc
    65521 ccgcaaacta ttggtattca tggacggcac tggaaatgat aaatcttcat caaccaatgt
    65581 gagaaaactt taccgactcg ctgtccagca tgcttgcagt ggaaaacgaa taattcccta
    65641 ttacgacaag ggtgttggcg cgaaattgat taaccgtgta acaggtggaa ttgggggaag
    65701 gggaactagt ttaaacatac gccaagccta taggttcttg atcgagacct acctgccagg
    65761 agatgaaatt tatatcatcg gtttcagtcg tggagcattt actgcaaggt cactaaatgg
    65821 actgatcgag tttgcaggtc ttgtcgatag aagaagcatc ccgccaaaat ggtatgacac
    65881 ttttgagtgg attggtgcaa caaaccttca cttcagcata ggcgcacttt atgacgtata
    65941 tcaccaggac tttgatggaa cggctgaatt cgaagagaga atcaggggag agctaaagac
    66001 cttgatggac agtcgcggca tgaccgcata caaggacaag gacaaagtta aggtcactgc
    66061 aataggtgtt tttgacacag tccctgccct gggattgatt cgcgatgacg agccagacga
    66121 ccacagatta gaactttacg ctgacaatgg tttccatgca ctagcactag atgaacaacg
    66181 agacgacttt cgtctgcttc gatttgatcc cttacgagcg gtacaaggaa gtcgacttga
    66241 agaggtatgg tttgccgggg tgcattcaga tgtgggagga agctattcac tagaatacaa
    66301 ctgtacatca aaagataact ttaatggcct tgcgacaacg ccattaaact ggatgctaaa
    66361 caaattttcg aagtttgaca tcttcccacc gacagacgag ttgatcaaat tagctgatca
    66421 ggagaaaaac ggatttcttc agtgtggctg cgcccatgag gcgaatccgt ttattgagtg
    66481 caattgcggg ctcttacatg acgaattttt tgatggtggt tttgggttct ttcagaacat
    66541 gggaacgttt ccacggaggc cgcacccagg cgataacatt cataggagtg tgcttcagag
    66601 aatcagatcc gcaaaaatat ttaagcctca tacccgacgt gaggctggag gacgctatct
    66661 gtttctaggt cgcaccatct cgggtaatag cgatgaattt ttcgccaaaa attttaaggt
    66721 cgtagagtaa cctcttttca atgagtatag gagcacgcgg atggcactta ggaaaatgag
    66781 ttcgaagatg cgacagtttc ttatcgccag tgctgtatgg gtgatggccg gagaacttgc
    66841 atggtcagga aatgactatc tcaatcctgc ggcaattgta tcccctcaga aggaagtatt
    66901 gtgttcgcag gtggatgacc acaggcttgc cataggagct tcatgtgtgg cctcaataaa
    66961 ggctgatatt tcagaaaatg ctgtcgggct gcatcttcaa ggaggcaatg agtacatcgt
    67021 gtcagtggat ggagaacaag cttggtatga cgcctcacac aagcatatcc ctccgcaggg
    67081 cgactcagga acattgctga tgaatctgtt tgcccttcta aaaaagaacc gcgaagcaga
    67141 ctggtttgca cttatagcgg aaatcaaagg cacgaacaaa tgtcaccttc acgatttgtc
    67201 gaaatcttcg aaccttcttg cagaggaaga tggctcgttg gttctctatg cgaatgatgc
    67261 tcgaggattt tattggaaca accacggtga gataactgta ataatttccc gaaaaaaatg
    67321 aaggttacca aaccatcttt atcgatatcg tagtttatca aaccgaatta tccgggtggg
    67381 tgcaattttg accacacaac atcctccagg tacagtttca gaccgtgcca aggaatgtgt
    67441 catccacagc aggtgaattg gttcagtcca tttccatcct tagccttaga agtggaagta
    67501 tcgtatggga agtaaaggct attttctgat tgtcgctttg actacttact ttgtcggttc
    67561 tgccgtagct gcttctttcg caggccctcg catagaatgg gaggtgggaa accgctatag
    67621 agcattcgat tacaaagcag gagtcaaaca atctgctgaa cagtcggcta agcaatttga
    67681 aggatacaga caggaagccg gagaaaatgt agagggctgg ttcaagcgaa taatcgagca
    67741 acagcattcg ccttacattg ctgatcctgg gccatggaat gaatccacgg ggaaatatga
    67801 aaaagatttc gtcaatgtgc cgaaacttgt tttcgtaaaa gcccatttgg taactgataa
    67861 agatcgcacg gaagttctga cacagaagcc ctgcatttgg cgtatcagca cgaatgaaac
    67921 gtctcagccc tgcaatgatc cattcgtcat tgccggattt gactctgggg gggccaaagt
    67981 ttctgtcctc caggatgggg ttgaaatcgc ttcagcggag ataaagcccg atttcaaaat
    68041 tattctgggg ttaggtgatt catatgctgc cggagagggt aatccagact cgcccaccaa
    68101 atgggaggaa aacgctaaaa gaaagtggcc tccggtgaac gatactgcta caggtgaccg
    68161 agtcttgaag actgctcagt ggtggtcaaa tcgatgcgat agatccttct acagttacca
    68221 aagtctcgcg gcactacatg tagcctccgc caatccacac gcggtcgttg catttgttca
    68281 tttggcgtgt tccggtgccg agatcattga cggactatta gcacctcaga gatttccacc
    68341 tggtcattca atgcatcgct gccaaccacc ttttgagcgg tccgacccta ataaatggga
    68401 taaacagtgt gatgttccat attctcaact caatgcagca gttgacctac tttgccctga
    68461 ggcttctaag cccctagaat ctgacaaaat taataaaatc aaatcagcat taaaaggtat
    68521 ttggcacaac aaagctcagg tgaattggct gaatgaacag ccaaaggaaa ggctgagatt
    68581 ctgtcagaag ccactccgca agattgacta tgtgctgata tctatagggg gaaatgatgt
    68641 tgggttttcg ggtctcattg cagatgcact gttgccgcga gacactcata ttagaacacc
    68701 cctgttgggc aaattggcaa aacagatagt ccaaattgtt caaaaagagg ggaaggttgt
    68761 ttgtccatac ctgcctgcag gaacgagccc ggaagatctc aagcggcaga aacaatgtag
    68821 ggagtatggg ttatcagccc agaccaggat ccacgacata ccgcagaggt atagagcgct
    68881 aaaactcgct ttagaagaga tattgcaaat ggaccctcac gagaagacgc tcaccccaga
    68941 taaaatcata ctcaacctgt atcccaatcc tctatacaaa acagggggag cgctctgcgg
    69001 gaaccctggg gggagcaacg atgagaatga atggcgcgcc attcatacag tcttacctcc
    69061 ttccaaaagt ctttttggat tgctaacgct ctacaactgg gaagtcaatt tcacagctga
    69121 cgaagcacgt gacgttgagg gacatgtcat caatccactg aatcgtgcgc tcaaagaaat
    69181 tggacaggag tttgggtggc aagttatctc cacagagagt gcgatgaagg accgagggtg
    69241 gtgtaccggt aattccagat gcctgtctca tgtcaaccca acagaatggt ctccgtatac
    69301 ctcccgcagt cgattcataa gaacggccaa tgattcctta cttacacaat ggcctaggac
    69361 aacaatggaa tcggaaagca aagactccaa gatcatcgag agagaaaatg gtttctatgg
    69421 gatgttccat cccaatgctc agggacacgc agccatcgca aaggtattag tagaccagac
    69481 gcagatcgct tcatccatca acactgaacc catgccggaa ctgctacttt ctagtagtgg
    69541 agataaatta ctaactttga cacaacgcga cagcgtagct gatgaagacg caacgtgtga
    69601 cagcaaggta atagttaaga aaaataagtg acccttttga ctgccgacca cccagtgtgc
    69661 gtcctggcga gatgccactc accaacacct tgccttccga atcattctca ttaggagata
    69721 cgcgaggtca cgttttacaa cgcccatttc catttaggat ttttgatttt gctcacgttg
    69781 agtcccactc agaaaaagcc gaagcactaa gtctctcgcc cagccccgag gttatgaatc
    69841 agcaattcgc agatttcagg atgatttctt cagtgctcta ggctttatct ccatgtgctc
    69901 aagctgcaac ccaatatgcg gtatttgatc tctcgtcacc acgccgacta ctccaaacac
    69961 ttccccatgg cattcctgga ggacatagtc acactgggtt ttcgcaaacg ctctctcttc
    70021 cctacttagc gactccgtgt ccacctcgat ttgcgatgca tcatttgcat ccctctgtcc
    70081 taagcttgtg tgttcagaca gcctgctttc aaaatccgta gcgtggaact gtagtacggc
    70141 attcaccctg aaccgcctac cgaaaattgg atgcttggtc caatttgata cttcgctcat
    70201 ctcaatgggt tcaagtgcct tgctaagctc cgccagcgaa gtcgactcat accgagcctt
    70261 gggaatggcc cgcggtggta atgctcggag cgccaggttg aatgctgtga tctcctcatg
    70321 ttccagacgc tcagcgattg gtttcataat gcgattgtaa tcctcccagc ttcgcctgca
    70381 ttctttaacc aaatctaccg ccacgccagc gccctcacaa agtcttgccc ggtctttcgc
    70441 ctgagggtca gaacaccccc aaagacaggc cagacaaaaa atagtcatga tgactcggct
    70501 cgaaagagcc acttggaaac ctaagctttc ccccatgcaa tcttcctccc atagcagaat
    70561 acgaagatgc tcatatccac ctcgtatggg acagtcaatc gaaatcggtt ctagcattgt
    70621 ggctgggtag tggcagaact atcttgtccg atcgctttcg accggttcct cctcgacctc
    70681 tgcctcttct gtaagtgatt gagaacgaac cgatgagaga ctatcagacc ctataatccc
    70741 tgatactgta ggggtttcaa atctcgctgc tctgccaacg aaacagtcaa cgtgatcagt
    70801 cacatcaaga gggtttcggt ggaccgaaaa gatcctgaac atggagaggc taatcctatt
    70861 aacgctgcca atacaggatc gatagaatag cccctacctc ggcccatcga gaatacctga
    70921 tcgagctcct ttgcactact cagagagcag taacccaact gcccatacta catgtaacag
    70981 cttgataatt cagggcataa aaatgtcatg ctttgcatta tgctgccatt ccaagcattt
    71041 gattatatag acttcttgtc agccctgaaa tagcggaatc tgattggcag cgcgtttccc
    71101 atcaaagcca cacacatccg tctactcacg caacgcaggt tctgcaacga caattttgag
    71161 agtctgtcca ccggttatct cgatttcagt ccttcgcagt cagtaataca tctgcatgaa
    71221 cgttatcttt ggaataggtc accaccccta tgcctcgcac aaccactttc tcgatccact
    71281 tccggaacga gatgctggag taccgcagtg aatccatttc gcttcaggcc atgttctgga
    71341 gatggctctg tctctttgcg tttcgacggt ggaaaacaga gttccgaggg aaccctgtcg
    71401 gagttcccgg tgagcgggat cccaggttcc cctgtcaaag ctatatgccg aggccgcggg
    71461 caactcacga catgccgcat tgcaggtttc cggacgggca ctacctctgt tggacttgta
    71521 cagcaaatgc ccaacttccc aatcccggca ccctgatcgc tctcaaaggg gcgagccgta
    71581 cggagttgaa gtctcgcacc caagccgttc cctcgactca cgaggcgcta tgaaaaagga
    71641 ttacgccgta tcggtcatct tccctgaagt gttggtcaag cacagccggc aacacatcag
    71701 aatctcggcg aataatctac ggctggctgc aaatgaggcc cttaaagcca tcatgaaacg
    71761 ggaagggatc aaaggacgac gactgactcg cttgcaactg gccgtgatcc acgccggcac
    71821 gagcacgata caggaccacg aggcctaagc gaacacacct catgagggct atgggtcttc
    71881 gtcttcatcc ccaggggggt agcctcctac gctgacaaat actcaccctc agttacagcg
    71941 cgacgatgct catgctctgc cgtcgtcagg aagccatggc cacagcggac aatgactcgt
    72001 ttggcgttgg catgacgatc atcgcagatt tttttgccaa ccagatttgt cggctcctcg
    72061 agccgaaaca gttagggtac accttcacga gtctgatcct gctcaacgcc acgaccatca
    72121 tcctactgga aatgagcatc ggctggacgg ccacgttcta ttcgctcggg ccgattggat
    72181 tcgggctcct cgatatcttg aacaagccct cgcgcttcgg tcctttatat ccgcaactgt
    72241 gggggtcccc tgggccatcc acctccacag agccatggcg tccggccgat cttgggcacg
    72301 gacggacact gatgtcagtg tgccgaagac agacacctcc tggtggatga gcgacgacgg
    72361 atgtttacta accggatggc gctcacgcgc agggcaatcg cctcgctgat accacgacgc
    72421 cctaaccgag cactgacaca gccctgagaa ccatgacgat gtctctccca tctactcatt
    72481 caaccgaaac gccacagcca attctctcta ttgggatgct ctcgcaacgc attcgtcgac
    72541 aactcgcgcc gatgctgacc aagcagacgt acttgctcca agcccaatgg atcaaacccc
    72601 aggccaataa gggccaaaag ggcttcttct cggtcactct ggcggacaca gacaatccaa
    72661 atatggcgat tgacgccatg atctgggaac cagcggccat caggaaggtg ctcgaacaag
    72721 gccaacagtt cggccacgac ctgctgagtc gggattctcg agtcgagttc gttgtcgagg
    72781 ccacgctggg gttttgggcc aataagaaca cgatctatgt gctcattcac cagctcagca
    72841 cgatcggcat gcttggactg cgacagcaac aacgggaagc agctcttcgc accctcaaag
    72901 aggagggtct ccttactcgc aatgccagcc ttccatggcc acgattccca ttgcgaatcg
    72961 gcatcatcgg caagcaagga agtgacgccg ttcacgatat cttaaatgtc ctgcacagca
    73021 gtccgtaccg atttgaaacc actattttcc acactgccgt ccaaggagtc ggagcgatcc
    73081 cagggcttct caaggcattc cacgatgtcg ccgcacacac cacaccgctc gatgtcattg
    73141 tcatgtcgcg tggaggcgga aatgaaatag atttggtcgc gtttgataac ctcgacgtcg
    73201 cccgcgccgt cgcgacagca agcaaacccg tcctgaccgg gctgggccac cacctcgatc
    73261 ggtcgatctg tgacgaggtg gcggcgaaag ctttgtctac gccaaccgct gccgggcagt
    73321 acctcgtcag ccatttggac cgcatccgcg aatgtctctt cacgacccat gcaacgctac
    73381 atgcccatac tcgacacacg ttgctggaat ggactcatgc actctcgctc acccggcatg
    73441 ccctctggga acgcgcccgg accacgatcg aggctcacgc gacagcgtcc caccatgccc
    73501 atgagcgatt ccagcgcgca ctccgcgatc gcctcgaagc cagctaccag caaggaagaa
    73561 cccttcgata cagccttacc gccagcctga ctcaagcgct ctcacagttg tccatggggt
    73621 acgcctttac actggtacgc ctgcataccg ccggactttc gcgcgtcgat accctaaaag
    73681 gcgccatcac cgcgtttctc caggccttga gcctctcaga cccacaacat cttcttgcca
    73741 gaggctatgc gtatgtcctt gataatcacg gacggatggt gacctctcgt aggacggtca
    73801 tcgctggaga gcagctacat attcacgttc aggacggcag gattaccgcc actacccttc
    73861 acacggagcc ttcgcatgag caatgaaaac acgtcccacc cccaactgcc cacgtcctat
    73921 gacgcggcat tcaaggaatt ggaggacctc tccgccagag ctacgaaagg cgaaatcaac
    73981 atcgatgagt tgagcacggc cattcaacga gggaaccagc tgtttgcctt tctcagcagt
    74041 ggcatcgcga agatcaaatc tgagatcgag gaccacgctc cggacggtat cacccaatcc
    74101 aagcccagtt aaccgcttgg catccgatgt tccatggacc atgtccccat ttagaagagg
    74161 actcactcag tcatgtcgaa acaccatcac actcacccga tcacgatcga tcagactctg
    74221 gatctcttag ccaagcagtg gacggctgca gaccgcatca gactcagaac cgcccggcac
    74281 cgtccacgcc atgtacgggc cgccgaactc tggacccgta ttcaatcgtt gactcaatcc
    74341 acgctggccc tgttccaaca cccttcagcc atggagacct cgcatggcca tcattctgat
    74401 cgttgacgac tattcagaga tccgagcctg tctccggacg atgcttgaag aggcgggaca
    74461 tatcattgtc gaagcaggct ccggccaaga agcactgcat gcctggaacg aaacccaccc
    74521 agatctcgta attactgatc actcaatgcc tgggatgtca ggtgtcgagt tgattcgcgc
    74581 tttaggccag cggcaaccgg aagtgaaggg catcttgatg tcagccgatc gggatccgcg
    74641 ccccgcggaa ccaccagacc agtcccacgc gtggccattc ctccagaagc ccttcggctt
    74701 ccgggcgcta cgacaagcca tcgattcgct tctgctggcc cagccggcgg aaccagccta
    74761 gagcatccgc caagcgtctt tcgcgcctct tcaccccaga catctgcgtt cgtccaccgt
    74821 tccggtctcc actgcatcac taacccgtcg cttaatggga tgtaccaggc tatggtcgac
    74881 tcagactttc tgtcgtatgc acaactctgt cagtttgtgc gggagcaggg acgtctccca
    74941 cggttagatg acccgatccc agcctatcgt tatgccggat gggccctacc aatgatttgg
    75001 gaagggcatc gcgtgctgcc tagggttcca aatcgatggg gctatcattt agacatactt
    75061 cacgcgagac ggattccgga cgccccgatt ccgcagattg aatttcttca cactccccat
    75121 cgagacacac tcacgcatct gcaacgctgg gtcacgctcg ccgccgagca tcagtccagc
    75181 tggacaggcc tcaccaatct cattgcttgg attgcgttct ctttaaaact taaccacgtc
    75241 cccccgtcgc tcgcagacaa cacgcaagcc gcgctctacc aagccgtcaa tctccaagaa
    75301 ttggtactcc acccttacga ttattggggc gatatcatcg cggaggggat cggccgtggg
    75361 ccgtgggcca atccaaacgg gttctatccg acccccatga gcgtctgtca gctcatcgta
    75421 cggatgatgc ttcccgacgc cgacagcctc tccccagccg cacggaacgc cttgcgggtc
    75481 aaatccattg cagatcccgc aggatccgga accggaagga tgctcctgct ggcatcgaac
    75541 gtcagcctct cgctctacgg gatcgaaagg gatgcactgg tccgcacggc atgtctcatc
    75601 aacggcgctc tctacgcccc ctggctcgcg ttccccttgc ctcaagaaat tctccatggc
    75661 acggacgcgg atcatggcct cacaaaccaa gcctcgctcc tctcagtcgc cacgccaata
    75721 cctgaagaga tttcgctcat tcctcccatc aacgatgccg tcgatcacga ggacctgcag
    75781 ctggtctttc ctggatggtg agcgagatac gctgacagca cgccaccggt gccgaccgac
    75841 ccatccggta ctggctcgcg ctgcgcgcca atgtgttggt tgtcacccag gtggggagga
    75901 agagtatgcg agtcctcgaa gcacacggcg acctctggga tgttcatgct cagggttctt
    75961 ggatcgccat cacaacaaac ggagtgataa gaacaaatgg gcatggcgtc atgggagcgg
    76021 ggcttgccaa acaggccgcg gaccggtttc ctagcctgcc ctcgctgcta ggcacccatc
    76081 tacgccgttt cggcaacatt cccagatcga ttccctccat gcggatcatg accctgccca
    76141 ccaaacatca ttttcgcacc gcgtctgacc tcgcccttat ccagaatagc ctgcagcaca
    76201 tccagctcat tctcacacgt gaacgcatcg accggctgta ttgtccgcac ccaggatgtg
    76261 gtctcggtca gctaagctgg cagcaagtcc gagcagcgat tgtgcacgtg gtcgatcatc
    76321 gattcctctt cctgcattct actgcttgag ttcctgctca tgcttccctg agcgtcggcc
    76381 attcgccttc aacagatgtg agacacgtcc ccagctcgca tctcttctcg caagctagac
    76441 gtcacctcac gacccatcac tgtcttcgta ccagaatcgg cctcaggcgt gcgtcgcacg
    76501 cagagtggag gatggtcgcg taccatccat tcacaagagg gagacttgcc atgcgtcgtc
    76561 aggcttacgc ggctatctcg ttgacgctat ttctcatatt gcccgcatct ccttctcaaa
    76621 cattggcaca gaccaaaccg gaagctccgc tcacgggagc atcgtggcag caatgcctca
    76681 tccactatgc ccaaaccctc gccatcgatc ctgacctctt ccatgcgatc gtccaaaccg
    76741 agtcggcagg tcacccctgg gccttgggct ggagcgacca aactgggaga cgacatagtt
    76801 atgctgcacg tcgccaggag gatgcggtcg ccatgctcct tcgtcttctc cagaccgctc
    76861 ccaatgttga tatcgggatc gctcagatca gttcacagca cctgggctgg ctggccagac
    76921 accaccaaat ccagtctcgc gatcttctag aaccatgtgt caatctctac gcctcgagtt
    76981 tgatccttca gacctcaatc cagaagcatg gtcgcacctg gagagccgtt gccggctaca
    77041 acgggtccac cgcgtacatt ccggccgttt ggaaacattt ctgtcagcgt gcggcagatg
    77101 ctggctgctc ctccggacgc tcgacgcctc cgctcccaag tgcgccgtta cctcctccat
    77161 acgacagaga caccgttgag atgcagcgga atacaggtgg cacgccgata tcgtggaaga
    77221 tcagtccagt cagcaggcag cgttcgtttc agagacctca cttagaagag accgcaacac
    77281 acacttttga tctcctgaac gtccgcgcac tgctagtcgt cacccttgct cttctatgtg
    77341 gtgcgattgg cctgctcctg atcctcgcgc tcggtgggcg cctcgtgctc ttggccgtct
    77401 acatcgttcg agaagggcta tatatgcttc gccggcaacg cgccgcacat catcgcctga
    77461 cagggtagcg tcacgaaacc cagtgtgcgc tcgcactcac cggctcacca tgaggagagg
    77521 atcgcccgtg aagactgctc tcgatcaaca ccagctcggg atctacgacg ttccgcgcta
    77581 caccatcagt cttatcaaag aggccgcatc gacgttccac gccaagactc gatgccagtc
    77641 atcggaagac gtctaccgat acttcaaaga catctgcgaa aaccaagatc gcgaacatct
    77701 ttatgctctc tttctcgata caaaacatgt gcccatcggg atgaatcatg tggcaatggg
    77761 cacactcagc accacgatcg tccaccctag agaagtcttt aaatgtgcca ttctcctgaa
    77821 cgcggcttgc ctggtcatcg ttcacaacca cccgagtggc gatccctcac cgagccccga
    77881 agaccgggcc ctcacgactc ggatcgccga ggcgggccag ctcctcggca tcgcactcct
    77941 cgatcatgtg gtgatcggca acggtaggta tttctccttc gcggaccacg gaccactgac
    78001 gcaacaggct cccacataca cgtcctaaga tcactgcgaa ggttcttccg acgtcccatg
    78061 agctttgatt caaaacgcaa cagcgggagg tgtccgagag gctggttttc gacagactct
    78121 gagcccacag cgtgtggtta atatattgaa actcggatga aacaggagag aatcaagata
    78181 gccccattag atgagaaaac tgtcttccat cggctggtgg tgttgattgc cgttccttcc
    78241 tctcgacgca gcatttatcc aaagagcaca accatgcatg cacctatgcg cgattccgtg
    78301 acgggacgat tccggcggcg ctattcctgt tcctcttgtc agatgcttcg cgtgaatgga
    78361 ctgccctgtc atgaaaccgg ctgtccagat agctggatag acacgacacg ggaatgccgg
    78421 aactgcgggt gcgacttcat acctgattcc cgatggcagc actattgcga cgacgcctgc
    78481 gcggcctggt aacgctagcc gattcccgct actcaggccc gctctagcgg gcagtgggtg
    78541 cgcgttcgtg aatcccaatc gttctgtgga ggctccttat gggcgtcaca aaaaccgcga
    78601 gcacgcttga tggtggccta accccaatct gtagttcttg tggtattgcg ctctgttggg
    78661 acatccctga atctgaatac accgagcgcc aagccttctg ggatgcctgg cggtgtcggg
    78721 agtgccatcc tgatgccaag ggggcacggc gacgctggat tgagcaacaa cacgcggctg
    78781 gcagcggccc atcgagggtg ccatgacacc ttcgatccgt tgtcccttcc gacgccgctc
    78841 tattcccaga catcacccct tctggccccc aaattccaat cagatcgaca gcgggatccg
    78901 aaccatctac gatctgagca agggcatcgc cctgttcggg gtgtttgtcc cattggtgac
    78961 atttaacggg gtctggtata aactggtgtt agcggtttcg gctggtctag ccttctttct
    79021 caccgcatgg tatttggaag gacgcaatgg atctacattg tgatctcgac accgcagttc
    79081 tgattggctt caacttcatc gggtggagcc tctttctcgg cgtcctgctg aaggatcgcc
    79141 tcacgcactt ccaacggaca cagacctcgc aagcgacgcg ctaatccccc gcatcccacg
    79201 tcgatgggtg caacactcct acacgccctc gcatcatcgt gtccgcatcg atgcatagtt
    79261 gtcctgtaat acagagacag gattgtagat tacggagact atgcatctcg aacacctgtg
    79321 tcgcattgcg ctcgaagacc ctcacctggt tgaaaccgcc ttactgcgag atcccttcac
    79381 ctcgcaattt ctgaaggact gtctcgcgca ccttcaccag ggagatccga aacaactggc
    79441 ccgtgagtcc cacctttttg ctcaactcgc taaaggcaca cttcgacttg accctcccag
    79501 ctagtctctc aatggctccg ccgtcaccac acccgctcgc acttctgctc cggacaggag
    79561 tggaatgcgg ctttaccgat ctcgtcgtcc gcgcatgcta tgtccagatc gcgacgatta
    79621 ctgaccctgc ccatcggatg gcgtggttcg acatcttctt ctcgcagtgg accacgtccg
    79681 cccaggttcc ttcgtgcctg cacccccagc tccgacgcac cgcgttgatc agaatcgacg
    79741 cgctccgatg ttcctaggcc agacagcttc ccctcaacac gtggcccgct ccgcgggcag
    79801 agcggaatac gtcgtggtct tacgtggcag accctactca cctcccaccg aaggagctca
    79861 tatgtcaggc aagcgcgatt tgaacttcag tttgtattcc ggaagactga cccatccacc
    79921 agaaatgatg cagtacaaca ccggacacgg ccatatgctt tgtttctccc tcgccattca
    79981 ttcggatgcc ccctcagaca aaatcgacaa tcccatgttc ctcgatgtga aggtcaccgg
    80041 agcttcaccg gaactccatc aacacatgag cgtcggccaa gaggtacgcg tccgtgggga
    80101 gctgcagtca cgaacgttca agaaacaaga ccgcatccaa cggacctact atctccacgt
    80161 gaaacacccc tccgacctcg aattcctgtc gcggccgaag gacaagacga cgacctaatc
    80221 accacaccca cggattcaca gccgcgcgac gtgcccatcc aagtcttaac aaggagtgtc
    80281 tcatcatgac cactgcccaa atcacgcacg cccagatagc ccgcgccctg tacgaggccc
    80341 actcctccgt ggagtgcctc tcgatcgaag atcttcgcga gctgcagctt tctactctcg
    80401 gttccaatgc caactctgag acatcctggc ttcccgcact tccagaccgc gttctcgtca
    80461 ccaccgaggc ggaagaggaa gaagccatca ccctcattga tcacctcttt caggcccata
    80521 tggacgcctg gtctctgaag gaccgcgatc aggtcttctc ccgaggcacc aggttcgacg
    80581 acgagaatcc cgccccccaa tacccactca tcacgaagag ctctcgccgg acatcggatc
    80641 cgtccttcat ggtcctccca gacgggcgaa gggtactcca actcgatgca cagcggttgc
    80701 taacactctg tggcatccaa gaagtgctac tccgagaaga agttcgacct ggtgcattcg
    80761 cacgcgccgg tcgaggcaaa tgtccctcct gccacggctt gcatctggcc actccccaat
    80821 cagcagaggc caccccagcg cgcgcatcag gaaacgacct cgtttgcccc agatgcaaag
    80881 tcagcattca gagcaactat attcaggagg atgccgatac cctgaaatgc ctctgctgtt
    80941 cgcaccgaat ttatcgagaa catgcccccg agctgtccgg tctcgatgcc gaggatatcg
    81001 cgcagaacga agaacgagtt gcgcgagcct gctatgtaga ccacggccag caggccgaag
    81061 ctgacgacga cgccctcgat ctcgacagcg gccaggacag cgaggatctg ttcgacgaca
    81121 ccacgcagat cgacacgttt gagtatctag attatgagtc ttcccatgaa attgcccaag
    81181 acgacatccc gaacgacgag agcgacgtgg acttgcctga tctgcaagac aagctccacg
    81241 caccggctta tgccctgttc tgtcagggcc gtcacccact tcgacaggcc ctcctcaacc
    81301 aggccatcct cgagctcgac gcccacgaca atcgcatgcc ggtctgggtt gcggccgggc
    81361 gtatcctcac gtccacccca caccacctct ctccgaccga tgcgcaggcc atcacaagct
    81421 ggctccatgg gcttcctcag gaggacctcg ccatcctggc tggcgacccc aacgccagca
    81481 acgaacgctt agcgcatccg gaacccgggt tgttcttccc aaccaatcaa ctcctgctac
    81541 cattgattcg cttcttcgga cacagtctga aacgctttcg caacaaggag gctggctgtc
    81601 gcatcctcgc cgcggattat gggctgacag aatccgcgct caaacacatc gccatggcct
    81661 acgggcaact cccagccaac cgcgaggcct cccacgtgcc tgcagcctag tctcgccctt
    81721 agtgatccgg cagtgcacat ccctccgctc aaccatgtga aggagtcttt tcatgaccta
    81781 tgctcacgtc gaactcgttg gccgtgtcgc gaccactccc gtgcgcgcca cagtcggcgc
    81841 acgccgggtg accaatctcc gcgtcgcgat cgaccggcct attcagccca ctcgtcctgg
    81901 acacactcca ccactgtttc tctccgtttg catctgggac cacaccctcg accaccagct
    81961 cccctccctg acgaagggcc aaacagtcaa ggttacagga cgcttggata cccgcacctc
    82021 ctcgcacacc ggactgacgt atactcatgt tgtcaccgac cacatcgaac tgatgccccc
    82081 ggagatacac cgacaggctg cctaaacccc ggccggcggc aaggcaaaaa aaaggggagc
    82141 acttccggga agacgcactg agttccaagg aactcgagtc ttccacaacg aagtgtctcc
    82201 cctcatgtac tggtgtcggt ccttacatgg ggccatgcgt aaggagttct agtccaaaac
    82261 tactattccc acgctgtccc atacagtgcg cgatgagcct catagagcgc atcggcagct
    82321 tcttcacgaa tctgcttcac cgagtcctga gtgacgcccg tccgacaaag cagctcatct
    82381 cgaagcgggg cttcaagggc ttgcgctctc tcccagacgg ctgtcacgaa ggcggcttca
    82441 tacgtctcag ggatgcagct cgatccgtgt atgtgttgga caatctcgtt gatgatggtg
    82501 tcgatcacat tcatgaaagc ctccaggcat agttgacaag ggcacagaga gaggcgtgct
    82561 ctccaagaaa ccacgcgctc cctctgctgc tcccctatcg acacgggcac taggcactcg
    82621 cggctcgaaa tacgtccggt gagacaccaa acgacggggc caaaattctg acacccgcgc
    82681 tggcaactcg gaatcgtctc aatgccagcc gacatgctag ctgccacttc tgtatcttcg
    82741 tcgatacgga cggcacgacc aacatacgtt ccggctgagc caattctgcc ggcaattccg
    82801 actctgggag aaacgcgcgc aactcctcgg tcgacgttcg catcagccaa cgtgcgattt
    82861 cctgttgctc cgcgcgaccc agcccacggc acgattccat gagtgtggac gctacggccc
    82921 aaatcatatc aagccgctcc ccactcagtc gttcccgcat cgtgccctct aatagtgcgc
    82981 aacgaagggg cttgaccgtc cagtcttgcc aaaagcgatt ctgcaaggtc atcagctgat
    83041 ctacaggatc ttcggcgctc tccatatcct gcgtctcaaa gtcctcaacc acactgcgtt
    83101 gagtctcctc ctcgccggcc atgctgctcg gcacgatgtc ctgttcgtca tcgtctgctg
    83161 attcttcaga ttcgtctatc ggccccgcaa gcgttccgtc tccgtgatcc tccagctcaa
    83221 gatccgattc cacatcgacc gtcgcactcg gtttgcgtcc gagttcgaca cgccacgcgc
    83281 aaatcagaca atgcgcctct ttcagatcta actgatggac accgaccgtc atcatggaca
    83341 ccaccacaga cgacgagcca cagtcgggac acagcggtgt atcgactgtc gcatcgatcg
    83401 cttgagcagc acgtgtccca cgaggagcag gcaccgtgta atccagttcc gccatgtctt
    83461 cccacgtctc accacgatcg tcatcgtcgt cgtaacgatt ttcgaacggt gtgcgagcgt
    83521 tcaacatctt ggcattgcgc cgttcaatat cggtcaagtc tgccaggcgt gcgcgcagac
    83581 gagcccattg tgctggatgc acctgataac gtggtttttc cagctcccag actagtcgtt
    83641 gcaggtcctc cagagcgagt cgttcccagg catgcaccaa gcgaccgtga tctgcagcga
    83701 ttcgttggaa atcttgaacc actaccgaga cgatcgcctt tgccatctcc gagcgaaccc
    83761 acgttgcatc caccacggga gtacggcgag gggctgcact gagaagtcca cgtaacgttg
    83821 catgcttcga tgtcagatgg gatgtcgtca tgatgagatc ctttcatgtc atggtatatg
    83881 ttgtgatgtg ttgatttcag acgtgcttcg tggcgcattc caacaccatc gtgcgagaca
    83941 tgccgtccca ttgaccaccc tcgacccaac tgctagagtt ggcgccgtgt tcagtcactc
    84001 tgagaacacc atgagactca cgccggaatt agagatcacc cgtcagctct ccacctttgt
    84061 gaacagccgc caggagctac tcatcgccaa cgcgcgtttt gtgggatcac gtgaattctg
    84121 tgcactggaa ggattcagcg aacgagtgct ctctcaggtc aatcatctca cccaagcgct
    84181 ctcactcacc gaacaggaac aggccctcgc tcgagacatc acagacgcag ttgccgcttt
    84241 gtctcgctaa tcacttccaa cgaggtccaa tcgctttgcg gcatcagcac gaaatgatcc
    84301 gctcttgcct tcgaccattt cagcgcccca gcctcgatca gtaattcaaa gcgattcgcc
    84361 agatcgttaa atgccaattg caatgcatcg ccggatccat gcagatccac cagtaccagt
    84421 ttgccgacaa ttcgcatgtc cacactgact gcttgatttg tgtacatgga cgcctccggg
    84481 cacctgacgg cagcatctcg cgatcctgcg tcacgtgcag catgtttaga atgtgtgtgt
    84541 gatgtgattg aacggtgatc ggagcgatac gtaaacggca gcttacgtac caccgatgga
    84601 gttaggagac gagacggaag gactcaacgc agcgaactcg cgtcagcgaa ccctatggca
    84661 cagctgttcg agctgtttgc tcaagagccc ggtctcgatc agtatcgcgt gagcgacaca
    84721 gacactgaac cgccggcgga caaggtacac gcatcgtcat acagacctcc ttaatgaagc
    84781 atgggcacat ggctcatgca tggtgaagag gatcacgcca aacatcctgc cttcaagtct
    84841 ctttgcctac acaggaagca aggagaacgt tagacctgga acatggcgtg aacctaacgt
    84901 ggaccgatcg atggaattga gagatggagc actgacagaa gagaagcaat ctagctgtga
    84961 tgatcactca caaggactag actgcggatg caaagacgtt gtaccgagta caacgtaaga
    85021 gaaaggatgg ttcggcaaag cctacgaaaa agaatatgtg ctcatggtat ccaagaccga
    85081 ataacgagtc aagccctcga cgtccgcctg gctacacaag attggatggt caggatattc
    85141 gtgaacttaa taggcagact ttgcacgaca agtcgtagtc acgacgaacg gatcgaggaa
    85201 gggacgatga tgatggagac atttgaacga actgaaggcg aagaagtcat tggcagtctt
    85261 ggcgaccagt tagtggaagc caatgcgaca tcaaggcgtt acgtggagct cttctctaca
    85321 tatccacgga attgcagaag tcactgacaa cccaagcgag actgaccgcg agggtgaggg
    85381 agttgaccac gtacgtgaga caagatggaa ccaatattgg tcaaacacag ccaccgaagc
    85441 aggaaggcct agttcagcag actcacgacc atccatgatt ccttcactcc ccatcctcac
    85501 cggtgcgacc gcccaacctc ctcacaggtg tcccgaggcg ctcaggatga gcgcaggtcg
    85561 ggcagctggg ttgtccattc tatcagccat cgaggaggtc ctatgcccca ggcaccggtc
    85621 gctctccatg ccagtcgcag tggcaagcct gcgtcgaccc gcgcgctcct cctcatcgag
    85681 caatttcaga ccgcgctcca ggacgaactc cagagtcgca cccctcggca acgtcaggaa
    85741 actatctgcg tcccgctcac agacgggcaa cggcaacacg tcactgggcg aacacactac
    85801 tatctctttc aatctgagcc gattccggcc aacctcgttg aagacacgaa tccttccctg
    85861 gtccttatca gccagcgcat tccctgtcga atcgttggct tttccccaga cggcctcgcc
    85921 ttagcgttcg agggcgaagc cccggagacg atcccttcgg cacatctcac cttcgactca
    85981 gtccgcttgc tgcaagccct cgacaaacgt ctgcaatcca tcagccagca accggacacc
    86041 ttcggaaccg ccctcgctct caaggccttt gatcctgatg ccatcgccac gatcactgct
    86101 ctcgaccgct tgccggaagc ctcaggcctc aatcccgagc aggcgcaggc ctacaccagt
    86161 gccctcgaac gagatcttct atttgtcttg ggccctcctg gtaccgggaa aaccgccttc
    86221 atcaccgcct tgagccaggc cttgttggtg cagaatgggc gaaccctgat ctgctccccg
    86281 accaatacgg ccatcgacaa cattctggag catctgctca gccaatctcc cgatctcgcc
    86341 atcgatcagg tcattcgtat cggcaccccc agtccagaca gcagtgatca gtgccaaatg
    86401 gccaatctcg agacagttgc gctcacccac acactgccgc tcgaagaacg cgcgaagacc
    86461 cttcgccgcc aattgcagga cttggatcga gacatgccgt acctcgctca cagcgccgac
    86521 tccgccaaac gactggacac tcactggcga gaattacaga cattgcgcga gcggcaccag
    86581 atgctccaca ccgatgaacg ccgtctgcgc ggactcatca ccgagcgggc catgaccatt
    86641 caagaacttg aaggcgagct tcaagccttc aatgccagtc ctgccttccg tcgtctcttc
    86701 gagcacggca acaaagcacg gatcgtcaac gaccttgcgt gtatccgaga ctatcaggct
    86761 gccgacgaaa ttacgctccg gtccctgcaa tctcaggtgc tccactcaca ggtccggatc
    86821 gacaccctca atcgcgtcat ggaacaattc cgccaggacc accaattgcc ggatgccccg
    86881 tggtcacccg aacaactctg cactctccat gaggccatgc agggcatccg acatgatcta
    86941 caaggcaccc tcgatcgcct tgagaacgag atcgctcaga ttgaacaggc cctcgtgcgc
    87001 cgtagcagga tcgttgcgac aaccctgacc cggacctaca cctcacggct actcgacgat
    87061 cagcgtttcg ataccgtgat catcgacgag gccagcatgg ccctggcccc agccctattc
    87121 tgtaccttgg cgctggccac ctcgagggtg atcatcgtgg gagattttct gcaactggcg
    87181 cccattgctt cggccaagac acaggccacc aaacactggc tggccaggag catttacgat
    87241 atcgcacaga tcacctcagg acgagatcct cgcgtggtgc ccctcagcat gcagtaccgc
    87301 atgcatcccg acatcgcgaa cgtcagccgt cgtctctacg cacgcgctgg gctcagctat
    87361 gtctccgccc cgtccacgtt gacggaccgc agccagaccg ctgaggccgc gccggcacca
    87421 ggacattccc tcatcttctg caacaccgaa cctgctcacc ccgtcactgt tcgggacgca
    87481 aaaggctctc ccttcaacct ctatcacgca ctgcttgcag tccgcttggc gcaacaggcc
    87541 ctctccacgc ccggtcattt gcctaccgtc agcatcattt gtccctatcg cgcccaggtc
    87601 aacctcatcg aacgactatt gcgcctggaa gaactggatg agctcgtgcg cgtgggcacc
    87661 atccatcgtt tccagggacg ggagagcgac gtcgtaatct tcgatacggt cacgacggag
    87721 aatgcgaccc gatcaatgtt aggctgggcc tatgacgatg cggccccgca caaactgata
    87781 aatgtcgccc tgacccgggc caaaggcaaa ctgattgtca tcggacatca cggagcactc
    87841 gcccccctac agcacgggcc tcagccgatt ctgtgggaat gcatcgaaga agccaatgcc
    87901 aatgggttgc atctcgacgc gacgaccatt ctccaggcca tgaccttctc actacatgac
    87961 agccccattg aatcggacac agtcctctcc cgggtattgg cacgtaccac cgcctcgcga
    88021 gggcagctca agcccgtgcc atcacggctc acatccctcg tccaagcctc ctagccaaca
    88081 cccctcgcac aacggccgcc gcattctccg gcggcagcat gacaactgcc ggtgttcgtg
    88141 accgacgtct atctccatct gatgaccggc cacgcccaca tccaatagga ggcccctcat
    88201 ggacacctca cccactcctc gtccgcttac ctatgatgaa aaacgcgcat cggaagccgc
    88261 ctttctcggc ctccccgcgc acccctcgtg gacgcaaggt gctcaacgca tctatgtcaa
    88321 gctcacgcag gctctggcca agaagcagcc gccgacggat ttgttgaacg aggacctatg
    88381 cccacggcta tcgtcacttc ctgccgtcaa cgagaccacc aacttgacgc cagaccgctg
    88441 ccaagtctgg cacctctcac tgcacgcccc agccgaacaa cacctattcg tgctcgatcc
    88501 acagcatccc ataaacgatg tcgtacagac gatcgagacc aggtggccag gtcgcgccta
    88561 tcaactcgac cccgtcacct gcggcgtgtt cgcacttctc aacattccgc aggaagcttt
    88621 caatcaacat ccggagcgac agatcatcca ttcccttatc gcaccggctc gcccgtcatc
    88681 gtccacgtaa gacacggccc catgactccg tctaccaatt caccacagcg cgtacccgca
    88741 caacgcacca ggccgcacga aggcccgcgc agcgggcagg catcacagcg tcacgattca
    88801 ggcacatagg gtgcttgaac gaatcacgtg tgcaggaggt aactatgaac acatccaccc
    88861 agactcccca ggaaatgaag aaaccgtatc gcgacaccaa taaaatcgag atcgccggtc
    88921 agctcggcca gaatcccgag atcgtcgcgt taacctctgg ccggataatc gttaccggct
    88981 cattggcctc tcatcggaac tacgtgaagg atggccaaac ggccaagaag accaactggg
    89041 tgccgtttac ataccgtggc gacaagcccg aagagttcgc caatgcctta cggaagggcg
    89101 atcaggttct cctcctcggt accctcgact accgtgaatg gattgatgag tctacacaaa
    89161 ccaagcggtc caccttggaa gtcgccgcgt tcgagtacaa actgatccgc gagggcaagc
    89221 atcattaaga cagtgcgtcg gcctgccacc gggactcaga tcggccctcg ctgacagagt
    89281 cccggtcccg gccatgtggg tagacgatcc ccacacatcg atcgtcgccc gtggattctg
    89341 cggactaaca gaatcatccc tctcagtgta aggcgttcag gaaggtaggc tttgaagcag
    89401 ccaggcgggt tggaatccgc ttccctctaa ggagtagtga tgatcccggt acgacggcca
    89461 gtgctttgcg cgagatacgc tggcacaccc ctgagatccg gggggagaaa acgcgcccat
    89521 gcctagataa tgctcacgga cggagaactg aatgaagcca cgagtacgtt cttcgagaag
    89581 gcttgtacgc tagggtggca agttgtagac ggtgcttccg agaaatgaaa aatagacttt
    89641 tggcgtaata gcacacctga acgcctcccc gttcccacct ttggtctttc ccctagctct
    89701 ggagaccact atgcagaacg ctccgatcct tgcgattgtc atctgcgggc tcttcgtcgg
    89761 aatcagtgtc ggccacccgt tcctccgccg gatcttgcac aacaaaagac gacggcacaa
    89821 tcaacgccac ccactctccc tcgaactccg acacctcgcc cctgtctacc gtcccaatcg
    89881 gcatcgctcc tgaccgcacg cgtcaggggc tgacatctcc tgctggccct gcgggcagag
    89941 tcctcgctcg atgaccttaa tggaggtgta tatgtccaaa cccgctgcgg cgaaacggta
    90001 tacctttccg agtcccatga gtgtgggaat tgctctgact gccctcatcc gtgcctacgg
    90061 gtatcattgt cattccccgg aaccgtattc cgcacattcg tgtacctacc tcgtcgagaa
    90121 tccgcacgat gcggccgata cgggcgggta cacgatcacc cgaaaaacag cgagtgcaga
    90181 ggagtacctg ttaacaatcc accgcgccac tgcccctcat atgcggacgc tcatcgaacg
    90241 ggcacgtaac gaggccgcag cacagtccag cattcaaatc gtgtcatcgc gccgcgccaa
    90301 gacacaggcg gccgcttaac gccgagacac ccgtctcacg cctcgactgt gcaccttctc
    90361 gtgatcaacc cacactgcgt ctggatcgcc ccaccctaac accaggcctc ctgacggagg
    90421 gatcatgcgc tctactcgtc cacttctgat cgaactcctc tgtttcctgc tgcttgtcgt
    90481 caccaacatg tcttgggcac agtcccaatc catcacgccc attccagagg aaccttcaga
    90541 cactcagtta gtggacgaag atgtgaacat cgccaccggc ttctatgtcc gcgactatgc
    90601 cgtaggcgga tctcgcacgg tcaattaccg gaccgccaga caaatcgtga acgttctcca
    90661 caatgaattc gagaacactg tcgtggagac tctgccagcc ccactgttct actggtttga
    90721 ccgcgatggg accggccggt tctccatgtg gatcgaggct caaggcaccg ggtgcgtctg
    90781 tgatgtccgc ccctacgccg attcgtccct gaccgtggct ccacgggtgc cgtgagatgc
    90841 gcccatcccc ataggcccgc tgcgcgggca ggtgatcgtc aggtgtcacc caggaggtcc
    90901 accatgatac cacaccacac ccaggcccag cgtgcaagcg agcaggccag acggcttgcc
    90961 gacacattca tcggtcgact aacacagtcc ggatacacgg ccaccgtcgg tgccccagtt
    91021 aactacggtc acaaggtatc gctcgctcat ccctcccttc cccacacgct gcacgcggtc
    91081 ctctatgtgg gcaaagacaa gacctcgttc gtgaaggagg gcaagaactg gccggatggt
    91141 ttgtacgatg tgctactcca agagtttcat acgctgctgc tcccacaccc catgcctctg
    91201 gtggctccca ttacacaggc agcaggatcg actgttgcct acgtcgatgg ctcctattgc
    91261 gagcaagacc acaacgcgca cattggctgg gctttcgaaa tttggcgcga gggccaatcc
    91321 atcgacggcc aggccggctc catctcacac ccagacgcat tgagtcttcg caacgtggcc
    91381 ggggagtgtc acgcggtcga acaggtccta gaatggtgtc gggctcatga ctgcacggac
    91441 atcgagattc ggtttgacta caccggcctc gcccattggg cgaacggcac gtggcggacc
    91501 aatgcggtcc ggacacaacg ctatcgtgag cgagtggcat cctccagagt ccgcatcaca
    91561 tggaccaaaa tccaggcgca taacggagag tacggtaatg cacgcgttga cttcttcgcg
    91621 cgccacgcag ccaccaatca tgtctttttt cctgagttac ccctctccat tctgcctcca
    91681 cgcttgcgcc actcacaggt gacacctgcc cctggccagt aatacagctg tcgatagctg
    91741 agtgactcca ccgccaattc gaggcagacc cctccagtcc aggcccgcta cgcgggcagg
    91801 ccgcctcggc acgatcactc gcatgaatca cgctgtaaaa acacgtctgt cgggaggctg
    91861 ttatgcgcat gctcgctctt gtgatgctca tcctcgttct cggggttcat tccaatcttg
    91921 tgaaagcgga ggagatcacc cctaacaccc tcccgaccat cgaacccacg ctcatagaga
    91981 tgccgtttgt cgaaggacaa ggtgcgagct atcccgtgtc ggaccaggcg cgcgcctgtc
    92041 gtctggcgtt acccaaagct caactagccc tcgccaaaag ccacgcgcac atgctcagga
    92101 cgctggcagt acgcccgacc tccgatccga tcatcgacat tacgaaccac tatgatcgct
    92161 atactcgcat ctgtacggtc accattcgtc tcacctggcc cacggtgtac acctaccctc
    92221 cggtgaagct ggccacagat taccgcgtgc cgacgcccta acccgtcgat acccaagcct
    92281 catagaaacg ctctacgcag ctgaactctt cgaggaggtc ctgtgaaacg agtcgtgctc
    92341 gtatcgggta gtcgcgactt tccctccaag gaattggtca tggacgtgat gcggaagtcg
    92401 ttccgtgcgg gcgattgtct gatccatggc tgtgccaggg gagtcgactc gtgggcagaa
    92461 gaagccgcac gtttagccgg agtgcccgtg tcccgacgtc cggcagactg ggaccgatat
    92521 ggcaaatcag ctggtatgct cagaaattct gccatgctcc acgaaaccct cgaggcgccg
    92581 tcgtatctga tcctgatttt ctgggatggc cggtcgcgcg gcacgaaaca catgatcgat
    92641 gcctgtcagg tcagaggagc cacgtactct gtcgtacatt cataggaacg cagggaagtc
    92701 cctgcgacac acactggcac caaaggagat cgcatgggcc gcactacacc gctctgtccc
    92761 cagtgtcagg aaattggccc agacacggtg atcgaacact cgactctcgg tctcatccaa
    92821 tatgagacct gtcgagactg tacatttcac aatctctctc gcacatcctc acagggacaa
    92881 tttgtcatga gctgctatgc gacgcaggat gagctaaatc gtgaccgcta tgcgctccac
    92941 tatctcacct gtgtgcgtga catgaagtac cagatgtatc agatctccac ggcgtacccg
    93001 cggtacaccc tgacacgacg cggccaaagc ggccgcacct acgataatgc gcactatgag
    93061 ttttggtgta aggtggaagc gctcatcgag cacctgttca caccttgctc ggatggacct
    93121 ccgcagctgt cttcgttccc gtaaccccgt cctgcatctc ccacctgagg ctcgctacgc
    93181 tcgcagggtc ggcttcctgt gactgttaac cacaaggagg tccccatgag acacaccggc
    93241 tttcatatgc ccatatctga aaccttgaaa ctgcgcacgc tccgtcatgt aacggagcag
    93301 caatccgtcg ataccttggc tcgcctccgt gcacaggacc gagagatgga caagaaggac
    93361 gtaggcgcca ttgcccgcgc gatccgagct ggacagtcgc gcatcacact gtgacgtctt
    93421 gcatcacctt tattttagcg tagctgtctt cttccacaag aagaccgaca gcgcacggc
//
LOCUS       DAORRQ010000007        93012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_7_length_93012_cov_531.032779, whole genome shotgun sequence.
ACCESSION   DAORRQ010000007 DAORRQ010000000
VERSION     DAORRQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 93012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 93012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93012
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7_length_93012_cov_531.032779"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PK224_03670"
     CDS             complement(<1..639)
                     /locus_tag="PK224_03670"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRB14868.1"
                     /translation="MKEATAWFMRQAADDFSGAERRRLDAWRAQSPSHARAYHQVQAL
                     WNAPELQQAAARSVTSETIDALRRQAHHSWRRGSMAAAAVFLLVGMALWSDAVLMWLK
                     SDYSTGIGEQRVVTLPDRSIVTLNTDTSIAVLYESDRRQVELLRGEASFAVQPNPERP
                     FTVESRGIATTAVGTQFFVKDRKTDVQVTVLSGSVLVTDGKRRSDQAVRLAAG"
     gene            complement(806..1327)
                     /locus_tag="PK224_03675"
     CDS             complement(806..1327)
                     /locus_tag="PK224_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB14869.1"
                     /translation="MSASPSIDLERLFHEHQRDLLAMLRRMVGCQETAADLAQEAYIR
                     LADVPTSQIIASPRAFLFRTATNLALDHFRKQKFRGQRQTSLEEAEQVPTDTRPIDAQ
                     AHDKQLVATLEKALSALPNRTRAVFAMRRVYGYSYQEIAAQLGMSERAVEKQLVIAMT
                     CCQEALLSKDLPA"
     gene            complement(1518..4619)
                     /locus_tag="PK224_03680"
     CDS             complement(1518..4619)
                     /locus_tag="PK224_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRB14870.1"
                     /translation="MNLSAPFVRRPVATTLLTIGATLVGIVAFQFLPVASLPQVEFPT
                     INVSASLPGASPETMASSVAAPLERQFTRIAGVTEMTSSSTIGGTNVTLQFELSRDID
                     GAARDVQAAINAARADLPSNLPTVPRYSKVNPADGPILILALTSELVSRGQMYDAASS
                     ILQQKLSQVEGVGQVAVGGGSLPAIRVDLNPTALNKYGIGLGDVRQVLTSTNVNRPKG
                     QLTSDDRTWEIRTNDQLQQAEEYLPLIVAYRNGRAVQLSDVASVETSVEDLRTMGVAN
                     GTPAVLVIIYRQAGANIIETVDRLRAQLPQLEASLPGSMSLSIVMDRTPVIRASLHDV
                     ERTMAISVGLVILVVFVFLRNLRATLIPAVAVPVSLISTFGVMYLFGYSLDNLSLMAL
                     TIATGFVVDDAIVVLENITRYREQGLSPVDAALRGAKEIAFTVVSMTLSLVAVFIPIF
                     LMGGMLGRLFREFAVTLSTAILVSLLVSLTTIPMLCARVLKSEPAESHGWWYRTSERG
                     FGLLAGGYAASLRWVLRHPRTMLVVTLGTMALTVYLYILVPKGFFPQQDTGRLFGNIQ
                     AAQDISFQAMRQKLTDVVDIIKSDPAVASITGFTGGGGHAGTTNTGRMFIALKPLEER
                     GLSSDEVIARLRPKLAKVPGAPTYLQAIQDLRIGGRASSAQYQYTLQSVDLAELNTWA
                     PKVEAKLRTIQEIVDVNSDQQDRGQQSLVVFDRVTASRLGLSPQLIDDTLYDAFGQRQ
                     VSIMYTPLNQYHVVMEVAPQYWQNPSALHDIYVRSPNGAQVPLSAVTRYEPANTILSV
                     NHQGQFPAVTVSFNMAPGASLGQAVLAVDQAMHDIGLPATVRGTFQGTAKAFQSSVEN
                     QPWLILAALVTVYIVLGILYESYIHPITILSTLPSAGVGALLALLLFKTELSMIALIG
                     IMLLIGIVKKNAIMMIDFALHSERMAEGKTPQEAIYEACLLRFRPIMMTTAAALLGAL
                     PLAVGMGVGSELRRPLGIAIVGGLLVSQVLTLYTTPVVYLFLDRLRLRWVRARAAALH
                     PAA"
     gene            complement(4616..7729)
                     /locus_tag="PK224_03685"
     CDS             complement(4616..7729)
                     /locus_tag="PK224_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRB14871.1"
                     /translation="MNPSRLFIVRPVATVLTMIAILLAGALAYRQLPVSALPQVDYPT
                     IQVMTFYPGAGPDVMTSSITAPLERQFGQMPGLNQMTSTSSGGSSVVTLQFSLDIDLD
                     VAEQEVQAAMNAAATFLPRDLPAPPVYSKVNPADAPILTLALTSATLPLPQVRDFAET
                     RFAQKISQLSGVGLVSISGGQRPAVRIQANPRALAAYGLTLEDLRTVVAAANVNQAKG
                     GFDGPRRASIINATDQLFAGKDYKALIVAYRNGAPVRLSEVAEVIDDVENTKQAAWMN
                     ETPAVIVNIQRQPGTNVIEVVDRVKRLLPQLQGTLPSSVQVSVLTDRTTSIRATVRDV
                     QFELVLAVALVILVIFLFLRTLPATVIPAVAVPLSLIGTFGVMYLMGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENIARYIEQGESPLQAALKGSKQIAFTILSLTVSLIAVLIPLL
                     FMGDVVGRLFREFAVTLSITILISAVVSLTLTPMMCARLLRERRNEQPGRFSQASQRV
                     LDRIIAGYGRSLQWVLRRQRATLVVTGGTLALTLLLFLLIPKGFFPLQDTGVILGITE
                     GPQAVSFGTMGERQQALARVILEEPAVESLSSFIGVDGTNSTLNSGRMYINLKPLGER
                     MAGAGEIIRRIEARVAEVDGITLHMQPVQDLTVEDRVSRTQFQYTLEDADPEELNRWA
                     PKLLESLQVRPELRDVSSDQLNQGLASLVEIDRNTASRMGITPQLITDTLYDAFGQRQ
                     VSTMFTQLNQYRVILEVMPEFQKGPEALQQLEVRGSGGSVPLNVFTRVTETMVPLVIS
                     RQGQFPAVTISFNLAPGGSLGEAVTAIRQVTQELGLPPSIRGSFQGAAQAFQNSLEHE
                     PLLILAALITVYIVLGILYESYIHPLTILSTLPSAGVGALLALMLFRMEFSIIALIGI
                     ILLIGIVKKNAIMMIDFALEAERNEGKPPEQAIYEAGLLRFRPIMMTTMAALFGALPL
                     AMGSGVGSELRQPLGIAIVGGLVVSQLLTLYTTPVVYLALDRLAARFRRDSVQMPMPE
                     TVPAGPR"
     gene            complement(7726..8964)
                     /locus_tag="PK224_03690"
     CDS             complement(7726..8964)
                     /locus_tag="PK224_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HRB14872.1"
                     /translation="MAESVRFATLVKRWMIGLCAACLLAVGVYVLMTRSGEAQPRQAE
                     KRPAMGARSTPVVAVAAKTSEIHIYLNGLGSVIPMNTVTVKSRVDGQLMRVLFKEGQL
                     VHSGELLAQIDPRPFEVQLIQAEGQMARDQAQMKNAQLDLERYRDLYKQNFIPKQQLD
                     TQEALVRQYEGIVKADQGQIDNAKLQLTYSSITAPINGRVGLRLVDAGNIVHANDPNG
                     LLVITQLQPITVVFALAEDHLPSVFERLKAGKQLVVEAFDREQKRKLATGTLLTVDNQ
                     IDPTTGTVRLKAVFPNDDSSLFPNQFVNARLLLDIKQGVTVVPSAAVQRGPKGTFVYV
                     VNEAEQTVSVRAVTVGVAQGEEAAIDTGLTSGEVVVVDGTEKLREGSKVELRPAAGAV
                     PASQEAPAPGGEGRTRGKRT"
     gene            complement(9328..10224)
                     /gene="cynR"
                     /locus_tag="PK224_03695"
     CDS             complement(9328..10224)
                     /gene="cynR"
                     /locus_tag="PK224_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HRB14873.1"
                     /translation="MEFRHLRYFLAVADALHFTKAAERLPVSQPALSAQIKQLEQEVG
                     VPLFDRVGRSVQLTRAGSIFREHARRALREMELAQVAIAEEEGLQRGTLTVGVVQTVN
                     AYLIPEIVGRFSTLHPLVSLKLDELSGPDIEAGVKSGSLEVAIGFVPVVSDRIESQPL
                     FEEDFVLIASPRHRFAGRRHLSLSALAQESLILLPNMFCTRRLLDGCFEQAGVQPKII
                     VEMNSIEGILATVRRSTLATVLPRLSLGLARNQTLRAIALKNPTPRRGIGLLWKKGGY
                     RSGAAKALTDQVRAVVAEHWRS"
     gene            10353..11087
                     /locus_tag="PK224_03700"
     CDS             10353..11087
                     /locus_tag="PK224_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRB14874.1"
                     /translation="MQNTSGLIRSIQVGLPSSEHSDCADDPDSTPWTTGMFKRSTPGQ
                     IRLGRHNLAGDGQADLVHHGGVDKAVCAYPSEHWLYWRGILPPHRLTGGAFGENFTLE
                     GLTEADVCIGDVFSAGTAVVQISQPRQPCWKLARRWQIKDLAVQMERTGFTGWYFRVI
                     QEGTVEPGAPLQLMERPCPEWTVATANRIMHHERNNLAAAEHLSLCPLLSASWQQTLR
                     QRCHNQAGPDPQHRRFGTTHSSSSSF"
     gene            11098..11436
                     /locus_tag="PK224_03705"
     CDS             11098..11436
                     /locus_tag="PK224_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14875.1"
                     /translation="MKRLVLSACALTIGATLALNSWCAAENTTTINILSPHNGEKVSE
                     TFDIKYEILNAPAGNHAHVYLDGAYQKGFKGTFTRVAKGEHTITVKIADHDHKDAGTA
                     TDSITVKVGE"
     gene            complement(11511..12179)
                     /locus_tag="PK224_03710"
     CDS             complement(11511..12179)
                     /locus_tag="PK224_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14876.1"
                     /translation="MLHFIVRSSPHIPLRHLVAASLVGLLFIGLAPSPLNAARGGGKT
                     EVLPVQKAAPAAVIIEGLGNEAELRRQMRAKNGVAELGASTAQVMFSQAPTGTFGFIV
                     PKLLGLALVTQSPDLAVARVAPAENAYEIHKLADGSGMLVGFMGAELMTQVAPSQRPK
                     NVRIALYSNPSEKAPHIVAVPLVKLVADRMPIRLDAKNPNGPVLLDMDLQGSSTRAST
                     QGGQ"
     gene            complement(12321..12761)
                     /locus_tag="PK224_03715"
     CDS             complement(12321..12761)
                     /locus_tag="PK224_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB14877.1"
                     /translation="MGMAWPFGLVADEKVCLGRVLVVDDEDSIRSLLRMTLTQAGYDV
                     EEAEDGGRAVEVLNSGDNPLMVDVIICDIRMPRINGVEAIAYFRAQYPSLPVIVLTGY
                     HDEQLASSLRKQGVVECLEKPAEREQILTSVAQAIAGRRISFYP"
     gene            complement(13075..14163)
                     /gene="ada"
                     /locus_tag="PK224_03720"
     CDS             complement(13075..14163)
                     /gene="ada"
                     /locus_tag="PK224_03720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HRB14878.1"
                     /translation="MTSSASTTRRINRNIRQDPRWAAILARDPAADGTFYYSVRTTGV
                     YCRPSCAARQPLRENVRFHATCADAEQAGFRPCKRCRPEQPSSVDRQAAMVVAICRYI
                     EQAGHLPSLDELAQRAKMSPSHFHRVFTSVTGLTPRGYAVAHRATRVRFELERDCPVT
                     EALYQAGFNSSGRFYEASNALLGMTPSRYRAGGAQTEIRFAVGDCSLGTILVAQSERG
                     VCAILLGDDPDALTRDLQDRFPLANLTGGDAAFERLVSTVVGFVEAPGRGLKLPLDIR
                     GTAFQQLVWQALQRIPAGSTATYTEIATRIGSPKSVRAVGRACGANPLAVAIPCHRVV
                     RNDGGLSGYRWGVERKRMLLERESRVAP"
     gene            complement(14267..14632)
                     /locus_tag="PK224_03725"
     CDS             complement(14267..14632)
                     /locus_tag="PK224_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14879.1"
                     /translation="MTRRLSKQWQVRGWTCAVLLAATFTTGCMHHPGGIAPSTKPLAP
                     GGYTELGKVRGQDCVYHLLGLIPVTGGNEMRNAVEDALRTKPLADALVEVTVDGYFQY
                     FILFSRACTQVYGTAVETK"
     gene            15682..16065
                     /locus_tag="PK224_03730"
     CDS             15682..16065
                     /locus_tag="PK224_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14880.1"
                     /translation="MLWKLKNPIRFWILFSALLLFTSTAASAEQDGRHHDLLRNAIEA
                     AGRGECPETLMTPLLIGACEDQQPGIGQRISSLGKIRKLRFMGIQNSQMGPAEVYRVT
                     HERGTMTWMINTDGNGKILVFYTPG"
     gene            16130..16708
                     /locus_tag="PK224_03735"
     CDS             16130..16708
                     /locus_tag="PK224_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14881.1"
                     /translation="MDIIYFRGAERLFIAFSGLTCIILGFALFRQTYSAVVSSPTEFA
                     AKGGGFELTLRNVWPGVFFAAFGMIILVASVLTQIKTQGTSMAEINTLYQSGGLPPSD
                     TKSRAAVAIAAIGQVLDTENKQPPDIEIQRTKNLSQLKTAQLDLVDVAYGEGSYTKFL
                     ELSSRSTDPKGFGTLSEEDRRFFEDLKTALDR"
     gene            17123..18133
                     /locus_tag="PK224_03740"
     CDS             17123..18133
                     /locus_tag="PK224_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14882.1"
                     /translation="MKKRVPIVFMFVLFQCFQQLPACAQISGGNYEEDLLSEKTKLDD
                     YIKNLRSFISVSGNSSLKALEGRVHFHVSDEPVPNAWVARDFQTGRNTIYMTAQYRLL
                     TTYLADADVIVSLDTNFHSCRQDYVDTIFKVLATNRQRSVSRGTQRRIPAPEVFLNTA
                     ANSCRPFQSRFPIDPKYRPARDQTVNMVVGLGYLHELGHIAIGHPPVSLSSIEQLPTS
                     SQRLAEFSRLMARSRLQEYQADDWAIDRFVDLSNNPLESLTNVLLTFYLAFSNLDCSL
                     EAADSHPNGYQRFARQMIRMKDRATSAGKFTNTQEVGRLIDDLLTFASKTQQKLDCPE
                     RG"
     gene            complement(19266..20126)
                     /locus_tag="PK224_03745"
     CDS             complement(19266..20126)
                     /locus_tag="PK224_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14883.1"
                     /translation="MPGFADDEASFIDAMQTASEGACYLVLKDFRPHLSDHQKATLQQ
                     IRIRITPSREADRVFARGEGSERRIEISVGFLMAGSLVSDLQVFQNLQNGQDRTWDYV
                     SYIATSLIKNSRADGERSSKIALKSFPTFLGFNATKERELYSDPRIYKIRSYLNVEAL
                     ALILAHELSHHFLDHVGKTELSLDLKRLQEAEADRLAVRLAIASGHNPLLGIYPFLLY
                     VAMEENFRIDPSQRDYPEPGCRVGGMIDAGIVSFYEDNGFIQHLKTTGEFETWDRSMR
                     TLREEFRLHC"
     gene            complement(20315..20827)
                     /locus_tag="PK224_03750"
     CDS             complement(20315..20827)
                     /locus_tag="PK224_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14884.1"
                     /translation="MNKRVRPWQYISALLFVVSMLTCRATYAADDHTEYAADDHTDEV
                     IDFEFEGSNAALFQNEIAFLESNGAQVNMVEKRDLHGLGPAGVVLVTLAGSAAICVLA
                     QAITRLLHELHTGGLGISITDGNIEVRERPEVEKGCVRIRMKDGTRIEKCSQQHQSLC
                     NILTDALRST"
     gene            complement(21464..22414)
                     /locus_tag="PK224_03755"
     CDS             complement(21464..22414)
                     /locus_tag="PK224_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14885.1"
                     /translation="MMKTPTLLITLVMLAGCAKPLVQYRVVTGSDMEGLTKFQLSDTA
                     IKFEYPIEGSGATAGPNTNKLNITSIPVVFTDTTYSVSGTSWYENWGVSTDINVSHKG
                     DTLLVKELGSSVSDKRVEAITTLVSAAEKALPLFAAGIPDISQVRQHPPAGILVSRLL
                     SSPPNNCTKLSDNSMKCTKVELEGGFILKDSSPVPYLADIDIAAPPAGSFERNKINFP
                     HKTHTFVYSACRTLHIRVTNEASPGFVAESSQVVADPAWLECLALPSKGKIATGSSCG
                     ADSVAQDSNLPGATEYINALVGAGKTIKDALDKQNKSGVK"
     gene            complement(22411..23295)
                     /locus_tag="PK224_03760"
     CDS             complement(22411..23295)
                     /locus_tag="PK224_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14886.1"
                     /translation="MLPSIKISIVPRCNQLSLAEAAAGAAAIQQQVIDHFAPVWKISA
                     VVTALERPEVGYVPVFIIDNITVPGLTGYHTVDDGSPYAVVEYGATWTLAASHECLEM
                     ILDPSADRRVSATLPSQAPKTVDFILEVCDPCQSKDCAYLIGRFLVSDFYTPNYFDSN
                     ATPGIYYSYNRKLVTPKDVRPGGTLAWVDTDSHAYQLTNQGGSVDIRDCGIFKPGPMA
                     AREFMYRFDQSHLELSHATADPTIAGDLHIHCKACEVATKSHCAKYEKDLCAKGLTRR
                     RKKSSSKRQKRTAKEKEK"
     gene            23644..23937
                     /locus_tag="PK224_03765"
                     /pseudo
     CDS             23644..23937
                     /locus_tag="PK224_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            complement(23992..24600)
                     /locus_tag="PK224_03770"
     CDS             complement(23992..24600)
                     /locus_tag="PK224_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14887.1"
                     /translation="MILLLPLALMVPYGTGIGLAQIVSDAIVVDGRRINMQKLTIQCP
                     FYHPEGEKVWNLHRRKYTLHLIDTRALADARGEALRPAENGYYAWSDNTWNTFITSEN
                     TFWLEPLTDVGFYIGTIPGLCYSSSCRNRYNGNVSVSMRRGISRNTLEMIIEYKLSCQ
                     LGDDAPTRSGCGEHITDKWDPPKDKFPCNILPYEAPPQRLGF"
     gene            complement(24763..25686)
                     /locus_tag="PK224_03775"
     CDS             complement(24763..25686)
                     /locus_tag="PK224_03775"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRB14888.1"
                     /translation="MHANMCLLRFALLGALIAMAAPVAAQQASQEARRQLSDTCKRTI
                     DEAMVKLHPGLIEYPELKNKSNLVLLVPAADFNAFANYESRQILIPVMWCIQTWFLVD
                     AHAHLHKNPQLRELVNDYTEYLRQRQNQSILSGPIDQILLESFDKFARVDALSSALPD
                     DRKLLAVREGIMVDSLAFVLGHEIGHLALNHKRYTEVTSAQARKQEDMADEFAAKLLG
                     RTGISIIPSMFTLVRFLQSEADIKGIPMTAKTHPRAECRMQRVLFSSGELDQLLSDPA
                     RRREFERGSTYSVPQFRRLMQELRVDCAGSP"
     gene            complement(25718..26086)
                     /locus_tag="PK224_03780"
     CDS             complement(25718..26086)
                     /locus_tag="PK224_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14889.1"
                     /translation="MTDERLLNLNVMASIRRKQCDGSFHSDLRAANPGNIVCAAFAIV
                     YREAVKAFGADVFIGADRFDGPPQSQDEYEGAIREFFRAGSVQQFEKLVEGADSGNPV
                     VNFKKAIYYSRSSGLINAAR"
     gene            complement(26634..27713)
                     /locus_tag="PK224_03785"
     CDS             complement(26634..27713)
                     /locus_tag="PK224_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HRB14890.1"
                     /translation="MWKVGDKPTLLTTSRLAREQQLEDMIVSDPRILSNEWMIIGRQE
                     RTSHGGCIDLLAIAPDGSLVLIELKRDRTPRETIAQALDYASWIAELTSEKIAQIYQR
                     FSGGGNLDEAFHARFGVELDEEALNQSHQVIIVAAELDPATERIVGYLNSHDIAINVV
                     FFQVFQQGHDQLLSRAWLIDPQETQANVVATKSRGEKEPWNGEYYVSFGDMTARNWED
                     ARQYGYISAGGGSWYSQTLKMLAPGDRVWVKIPKTGYVGVARVIEAVRPITEFTVSTP
                     SGERSALEVLKHANFYKPNADDAEKAEYFVRVKWLDSVPESKAINEVGLFGNQNTVCQ
                     PTTPKWRHTVERLKTHFPNWDESVN"
     gene            complement(27739..28545)
                     /locus_tag="PK224_03790"
     CDS             complement(27739..28545)
                     /locus_tag="PK224_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14891.1"
                     /translation="MRYIPVHELTAQLFSEPDGARHRVRLFQSHLSLVAMSPAERQRY
                     IRTNGRSKWSQVKNWLTDRLGKKCWYTEVELVGAPLAIDHFRPVCDYWWLAFETANYR
                     VACAFANSPEHNKLHGCAGGKGDVFPLLGPGLRAKGRNKLKYEKPVILDPCDKADCDL
                     IAFQADGRPVLNPAHAADAIAQRRVEESKILLNLDHPDFNSKREQLCNEIAHDVQMYE
                     ESAERAEARSIIRTRLKERIEARAPFSSAAKYYLRLHRHLDWVEALFAEM"
     gene            complement(28554..30023)
                     /locus_tag="PK224_03795"
     CDS             complement(28554..30023)
                     /locus_tag="PK224_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB14892.1"
                     /translation="MKLERLWIKDYKNLRNCEIDFPRPQLLNAVIGSNGSGKSNIIEA
                     ILHILLGVYFKKAPPFDFMFEFEAQNRRITLESDNRKMTVMVDGEEKSLRFFADRLRE
                     GAAQVYYPEVTLVYYSGECQRVNQLIGSYRRHFERLTRKPESDRNRPLFVQTTNAQAQ
                     VILLALYAHGHLDLLDRLGLKRLIDVVLVLRSPSSFDSKQHEPKLWNTVGAVRRVIGA
                     IHETASSEESRRKKRRPAPGTEDDMAYSEQRTYGFDVDKRADKGLTALATRLARGGDN
                     IYLALEHLRARGIFGSVSFRLIGRSNDEPFEFDQLSEGEKQLLAVVGALKLTNSPDNL
                     VLLDEPDTHLNPQWSWDYPSMLTDALDTERRSRSTVLMATHTPIMISGMTSDQVLLAR
                     HPSDAGPAFMRPRRHPRGQGIANLLCSSEYFGLPSSLDKETQKLLDERLAISIKEFLT
                     STEKLRLKTLNSQLEILSPGVSERDPAYVEFLRRRVVPN"
     gene            complement(30020..31657)
                     /locus_tag="PK224_03800"
     CDS             complement(30020..31657)
                     /locus_tag="PK224_03800"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRB14893.1"
                     /translation="MTLETFFEKFDLFTDAPDAVAKMRELVLQLAVQGKLIDQDPADE
                     PANTLLERIKGIKDRLISERAIRQQNTEPLGEDEAPFLIPYSWVWTRLGEIGDWGSGS
                     TPTRGNHELYGGGITWLKSGELNDNQALKGSEETVTELALKTCSFRLNQPGDVLLAMY
                     GATIGKVAILAEPAATNQAVCGCTPFEGVSNHYLFTFLISQRLRFHAAGEGGAQPNIS
                     KVKIIGFPFPLPPLAEQKRIVAKVDELMALCDRLEAQQQERETRHAELARASLGRFAD
                     APTPANLGLLFHKSYSIPPAELRSSILTLAVQGKLVPQDRNDEPALALLDRIAAHKAA
                     LSREGRLRGSTLVTPMAADQGDFELPSTWKWVRFGDIMVNRDGERIPVSKEERATKAK
                     TYDYYGASGVIDKIDNYLFDKPLLLVGEDGANLINRSTPIAFIARGKYWVNNHAHVLD
                     GISEDFLRFMELHINAIVLEPYITGTAQPKMNQAKMNSIPIALPPLAEQCRIVAKVDE
                     LTALVDLLETQLATASTTGSELVDAVVAELTQGRVSV"
     gene            complement(31654..33135)
                     /locus_tag="PK224_03805"
     CDS             complement(31654..33135)
                     /locus_tag="PK224_03805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRB14894.1"
                     /translation="MSNVSNIVKTIQDIMRKDAGTYGDAQRLEQLGWMFFLKIFDDRE
                     KELELLRDNYKSPLPKHLRWSNWAKDEEGITGEGLLDFVNNTLLPKLKSLTGGADKIA
                     GLIRMTFEDANNYMKNGTLMRQVINKINGIDFNASDDRHMFGDIYEKILKDLQSAGNA
                     GEFYTPRAVTQFIVEQVNPSLKKHETILDPACGTGGFLVCAIEHLRKQARTEADEAFI
                     QECFSGIEKKHLPHVLCMTNLLLHGIDVPSNVRHDNTLARPLRDWSPKERVDVIVTNP
                     PFGGMEEDGIESNFPSEFRTRETADLFLVLLMKLLKPGGRAGLVLPDGTLFGEGVKTR
                     IKEALMSECKLHTIVRLPNGVFNPYTGIRTNLLFFTKGQPTTEIWYYEHPYPPGAKSY
                     NKGKPIRIEEFEPERTWWGEESNGFAARVANEQAWRVSIDQIKAGNFNLDLKNPHNPD
                     TGPGDVEHLLPEYEKLLGQIAATRAALKAELQNALTASAGTAE"
     gene            complement(33139..35505)
                     /locus_tag="PK224_03810"
     CDS             complement(33139..35505)
                     /locus_tag="PK224_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRB14895.1"
                     /translation="MNKKELTEADIRTKFITPSLIGPEGKWDVMSQVREEFYFTKGRV
                     IVRGKTVKRGEAKKADYLLYYKPGIPIAVLEAKDNNHPVGGGMQQALEYAEILDVPFA
                     FSSNGDAFLEHDRTGIVGAVEREIPLGQFPSPGDLWSRYQAVKGIPAHQERVTAQEYY
                     DDGSGKTPRYYQLIAINRTVEAIARGESRILLVMATGTGKTYTAFQIIWRLWKSGAKK
                     RILFLVDRNILADQTKTNDFKPFGQAMTKITNRTVDKAFEIYLSLYQAVTGTEEEQNI
                     YKQFSPDFFDLIIVDECHRGSAAEDANWRKVLEYFSSATQIGMTATPKETKDVSNIEY
                     FGDPIYTYSLKQGISDGYLAPYKVVRIGIDKDLEGWRPEKGKTDKYGQVIEDREYNVT
                     DYDRNLILEKRTELVASKITEFLKATDRFAKTIVFCENIPHAERMRQALVNANPDLAA
                     ANSKYVMRITGDNEEGKAQLDNFIDPESTFPVIATTSQLMSTGVDAQTCHLIVLDKTI
                     NSMTEFKQIIGRGTRINEDYNKLFFTIMDFKRATALFADPKFDGEPVQIYEPGQKDSP
                     VPPDGVEESDEENPEGYAELGSRNGEEPPVIHERPAKYYVNDVEVTVATERVQYLDAE
                     GKLITESLKDYTRKTVRNAYASLDAFLNAWKNADRKQAIVEELSSRGVFLEELAEQVG
                     LGFDAFDLICHVSFDQFPLTRRERAENVRKGNVFAKYGEKTRAVLDALLEKYADGGIK
                     SVESLEILKVDPLTHFGTPVEIVKLFGGRSDYLAAIRELETALYQKAA"
     gene            36089..36400
                     /locus_tag="PK224_03815"
     CDS             36089..36400
                     /locus_tag="PK224_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB14896.1"
                     /translation="MEQAWLTVDELAAVLKVSPKSIRRAYRKGEIPVDRFCRFVRFDL
                     ERVKEALQAKGHGPSSVVSPRKGGQRSATGGGSRRRAQRTRPRLGKTGASIARTPRRK
                     K"
     gene            36397..37389
                     /locus_tag="PK224_03820"
     CDS             36397..37389
                     /locus_tag="PK224_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB14897.1"
                     /translation="MTASGGFTQTIDWLAFTLPKAEVDEVIKTIGGDWFQSETGFRGY
                     PVARLMTQGKTGVAKLGTGAPRNPKEVHVDLSAGIVSQWDETKLKTILAWIFAQKGHV
                     TRTDVALDDREATVAVETVRQAVEAGQVVSRSKQFKVIQASNHREGIRTGETLYFGSR
                     ESQSMLRVYNKRLELQAKGREDAVSYGVRWEMELKQDRAQACAKALLTLDSEDWRAFL
                     VGVLRSYVDFRETTREAESYEKYRAPLLGWWEALTEGFRRCRLVVERLQQRLDDVAAW
                     LAQSVASMLAVVVACRGDQFLTELIYAGTKKWKQKHYALLKERRSGTPYVLTIS"
     gene            37590..37853
                     /locus_tag="PK224_03825"
     CDS             37590..37853
                     /locus_tag="PK224_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14898.1"
                     /translation="MQVKAEGAVQGYVERRSREGKVYRSVDLYVKGRDPGVLRLGIPE
                     DQVPLIDACKQAEGKQAKVSIEVRKFEQTGRVFFDLYGLEVLK"
     gene            37945..39213
                     /locus_tag="PK224_03830"
     CDS             37945..39213
                     /locus_tag="PK224_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14899.1"
                     /translation="MKQCLLTSYLGLIWTLLISFCLAPGESLALETTQYSRVVAQAER
                     IAYLAAQRSALASQVATAALTPSASSFAIRLVAGPVGWAALGVGAGLVLAQMYYSQPD
                     LASIKTASSTPGGWQVESTNAGTQTFPGLGTNSPANANYPNASIQFSATNVPLCAVDT
                     EYQHDWVVGPFQSVSTAVFFTGNFFVNGPAVGGQSLYVCHRKGIPGSPAPLQDALIPP
                     TPQQAANYVTGLPASDPKSIEAHTNPVGTTGTTQPADNTVSQPVSPSEMPTTVKPKPV
                     PAGDIIVVDSVAPPASTPQQNTQQQTTTTTTTTTQNPDGSTTKQEETEATTSCTAGAH
                     ENRTFGTVLQAHQTIWASSGLLSTLNLLKSLTWPSTLPVIALPSALFGNQQVDFNQWV
                     WFFTVLRTIVIAIASFAAYRIIFVGGGQTT"
     gene            39210..39485
                     /locus_tag="PK224_03835"
     CDS             39210..39485
                     /locus_tag="PK224_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="HRB14900.1"
                     /translation="MTAILTLIYCWLQEFFFSLTDWGLGIWDSLLSVADSTLSTMGTA
                     GLTLPVIPDQYAWVLGATGMSQALAIVASAMGTRFILQTIPFVRWGS"
     gene            39565..40566
                     /locus_tag="PK224_03840"
     CDS             39565..40566
                     /locus_tag="PK224_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HRB14901.1"
                     /translation="MIELYEGVPGSGKSYHAICEKFLPWVRQGRRLYISVDGIYLDRL
                     ALFTGIELSVLEQQITIWKDSVEVLQAFPHVEPGSAVIIDEAQTVFRSMQKVEPGLLR
                     WLETHRHYGVDILLMSQDFRQMSQGVTRLIEATVKFRKLAFVGLSKKYQGKVRGNPED
                     HETIRAFVGTYSPAIYAYYSSYASAAIQEEKRSHTIFKSARIAIGIAAGLFAIGLLFW
                     RPWSSLNSTMATPATGVSGTATLPPSASASGSLVNPLGVSVSVAAIPQPPKRLVRILG
                     GAGSSANRQGWRYLLDSGEILTAAQITGRYGIAVSEVYEDGAMRVIGEGVFYGPAGD"
     gene            40728..40943
                     /locus_tag="PK224_03845"
     CDS             40728..40943
                     /locus_tag="PK224_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14902.1"
                     /translation="MKGVGWVKGLTGFLMALVFAVGVPTLSQAQLFPVSADVATVRAD
                     LLLWAAALIGVALAIYAFKRVKAIVGG"
     gene            41031..41159
                     /locus_tag="PK224_03850"
     CDS             41031..41159
                     /locus_tag="PK224_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14903.1"
                     /translation="MTAQQIQTIGNALATDLVAWGTAAIGLALVAAGAAWVLRLLR"
     gene            complement(41156..41272)
                     /locus_tag="PK224_03855"
     CDS             complement(41156..41272)
                     /locus_tag="PK224_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRB14904.1"
                     /translation="MTKRLRDAGDLFGIPLLDHLILAEERYYSFADQGWPGA"
     gene            41216..41347
                     /locus_tag="PK224_03860"
     CDS             41216..41347
                     /locus_tag="PK224_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14905.1"
                     /translation="MIQQRNAEQISSVAQPFGQNAIFWARGGIAGGVIMAACDVKSL"
     gene            41937..43448
                     /gene="ltrA"
                     /locus_tag="PK224_03865"
     CDS             41937..43448
                     /gene="ltrA"
                     /locus_tag="PK224_03865"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HRB14906.1"
                     /translation="MNVAHAACASSNGAPQWNQIDWADCERDVRRLQARIVKATREGR
                     HGRAKALQWLLTHSFSGRALAVKRVTGNAGKETSGVDHAVWRSPEAKAHAIGSLRRRG
                     YQPQPLRRVYIPKANGKLRPLGIPTMHDRAMQALYLLSLDPIAETTADHNSYGFRPMR
                     STADAIEQCFKLLSRKSAPQWVLEGDIVGCFDHISHDWMLNRIPMDKAILRGWLKAGY
                     VEKRTLFATEEGTPQGGVISPALANMTLDGLERVLQRAFPRKDLGRGRGKYNPKVHLV
                     RYADDFIITGTSEEVLRNEVRPLVEQFLAVRGLALSAEKTRITHIDEGVDFLGQHLRK
                     YHGKLLVTPSRQNVHAFLQKVRAIIRANCAAAQADLIHALNRVILGWVMYHRHIVATR
                     TFCKVDHVLWHCLWRWARRRHQNKNANWVLHRYWHPVDGRSWRFAADTSVRTLEGNPD
                     WLKLVCANKTIIRRHLKIRAEANPYDRTWRAYFEDRRSATGHSRRRLHPPPPL"
     gene            complement(43523..43897)
                     /locus_tag="PK224_03870"
     CDS             complement(43523..43897)
                     /locus_tag="PK224_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRB14907.1"
                     /translation="MSVDSSLGSSNDGRPLNPVRKYGVPRYRVTLVREGRAIPAAESV
                     HTSEGAAAILRPLFAGLDREQFLICGLDAKHGLIGINVVSTGSLTLAIVHPREVFKPL
                     ILMNAGAWICRVRMRPCSRILP"
     gene            44130..44423
                     /locus_tag="PK224_03875"
     CDS             44130..44423
                     /locus_tag="PK224_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB14908.1"
                     /translation="MVRTSQEQTQGNATLEIMTLAEAAIYLRVSKSTLYQRKDIPRHR
                     MPGSREVRFLKDELIAWLKSGDREALSVASRELDFPLDSAHATVYHRNPKYRG"
     gene            complement(45592..45667)
                     /locus_tag="PK224_03880"
     tRNA            complement(45592..45667)
                     /locus_tag="PK224_03880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45632..45634),aa:Lys,seq:ttt)
     gene            45779..47062
                     /locus_tag="PK224_03885"
     CDS             45779..47062
                     /locus_tag="PK224_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB14909.1"
                     /translation="MRTATEYKLHNKTNRDRLKIRKKPYFVSLSPGLCLGYLPRGGKA
                     TGRPGVWYSRRQVGERIITDTGSHSRYKMKQLGQADDLPGMTADGETILDYDQACVQA
                     RAHAAELIRGPIKTPLTVRKAIDRYLTYLEQRNGEDARYRTESKFNKWVLKHPIADEE
                     VNALTLSQLQNWQASTVIRSEDDPEQERRSQDTSNRVLRSLKAALNHCYDHRAETGVV
                     NKDAWGRSLRQFKDVGTQREYHFTVPALLRLIDKAESYFAQVLKAAFYTGARPGELRK
                     LRAKDFKPSLKQLSIAKGKTKQRVTTLTAEAVAFFSALAKDKTPDAFLLTKSDGTPWL
                     PGEHKLPMRHALVGAEIFTAKQLDDMPRHERPVVYSLRHTYISRAIEQGIPMLIIAQN
                     VGNSAGMIEKHYAKIIERTRQEWIEKGAPSLYATA"
     gene            47049..47672
                     /locus_tag="PK224_03890"
     CDS             47049..47672
                     /locus_tag="PK224_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14910.1"
                     /translation="MPQPETKPTRSKKLLLVESPSEEYYAAMTPDERAEYEADPSVED
                     LATWRQQDQQVLALVAFGGLVPSPVADKQQKWLKSVEGRSKGAISKNKELRETEQDAD
                     EAILNYLKECPLPHGRVTKGGAGKWFYKHTLWPKSWPKASENPGKGRRRPPTFAKDKH
                     GQPTKELIVQCPSPNVISREFLPLLEEHFKCQLYRRPTPRLSSPTKK"
     gene            47756..48043
                     /locus_tag="PK224_03895"
     CDS             47756..48043
                     /locus_tag="PK224_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB14911.1"
                     /translation="MADDQAEDTTSENTPLLADIRAAQLDTYKAQARAAIMTQPVFVD
                     AEVAQVLHVPLSTYFAIRRKLNIPSFKIGKKRLTRREDLLKWINSQAEATR"
     gene            48040..50244
                     /locus_tag="PK224_03900"
     CDS             48040..50244
                     /locus_tag="PK224_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HRB14912.1"
                     /translation="MSTPEVRGSTYEKAQELLTHELYLFPSEHKKKHIKGWPEKASRD
                     PKKIKEWLGPGGTHESWKISIFTGKLGDGKSLVVVDNDPRHGGDETIRAIEAQHPDAF
                     PPTREHRTAGGGSHRLYWTTWPGVKSGAGVLGPGVDIKSAGGLVHFGDGYDLINDLPI
                     AEASKYLLAQCKPVGSTSRPPSREQVLPSVAINQEHANSRAEWYLAHDAKPSIQGQGG
                     DQNAFFTAAKLKDFGVDESAATLLMWEFWNDRCEPPFYADELETKVRNAYRYGQNPPG
                     IDAPEVVFARSKSAIEDFNRDHALVTTGGGVEVLYNTLDRHGNPAFEHLDVGACKIKY
                     AARKIFDGKKDAPLFDEWLRSPIRNSYDGYVFKPGQPGDVIIDGNGHTKKFFNLWRPP
                     AFAPAPSGATHPAVDQFLEHSRYNMCRGAEELFRWLIAYCAQLVQRPWEKPHVALVFR
                     GGKGVGKNAWVECIRSVLGPHSLVTSDRRYLMGNFNAHLENCLLLVFDEAFWSGDKQI
                     EGMVKNLITGTEHIIERKGKESYAVDNLTRVVIIGNEDWLFPASPDERRIAFFDVGDR
                     RKQDHPFFKSMLEGMKSGGAAVLWRYLMDYDISGINLNLAPVTSGLLDQKLLSNEPWL
                     EYWRGCLRDGQIAGSEFEGWPSSIDCKRFQMAMRQALNGRNIKGWIPSEESIGRKMKP
                     LGLRRDRQRVRRQERSPEGPEFVYVYHIPELPEVRRLFDVFMGQPTQWEAEE"
     gene            51101..51316
                     /locus_tag="PK224_03905"
     CDS             51101..51316
                     /locus_tag="PK224_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14913.1"
                     /translation="MSDDDALSAIEEDNRMAEARALAGLNAAGDALLAKPRSPTAKRN
                     YRNAQAKLADVRLFNEQRIEHLTASKN"
     gene            51276..51911
                     /locus_tag="PK224_03910"
     CDS             51276..51911
                     /locus_tag="PK224_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14914.1"
                     /translation="MNNESNTSQHRKIRGDGTMALLQTIKTKMMLSDMDKLGNLRAQL
                     KAAESDRDAARAALGAALLDEVPPAAADATAQIEAADKRFRDLTAAIDTISRRIQVAA
                     DRKKEDAKAAQREVLRSALAKVQSDARAVEHDFETLHQHTKALQESVNEALSAAAVLG
                     DDARHSLLSNGRAKFKFYLLHHASVMPGCKTPYIKSLERYSENFPKPAELK"
     gene            51945..52607
                     /locus_tag="PK224_03915"
     CDS             51945..52607
                     /locus_tag="PK224_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14915.1"
                     /translation="MDEPTLNTTPNPATSDPAPNGQDAPSPEQATVANWLREDFNAGK
                     MTADQFAEAMQELTGQEDTQGLDMRTPEQKDYDASFPPARAEDFNFPFPEHGVSMTAD
                     MKAADTMARGWLATGLFTKEHGSALAVEVDRVARKIEHFTPAEHELFARSERLVLEKV
                     WGADTQKNLSIAKAFVREVAEKHPQIIDFLDQTGLGNSSGVIIGLYHQAERLAARNGE
                     SL"
     gene            52604..53050
                     /locus_tag="PK224_03920"
     CDS             52604..53050
                     /locus_tag="PK224_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14916.1"
                     /translation="MKTSQEMKEARYAAGIIKGLTKKQAAIEAGFSVSVAEHSVNKIH
                     EKPAVQAAIEQARTEIRAAAVYDLTRAMRESLEVIEFAKQHKNAMAYFKAVEHRAKLS
                     GLLIDRLDVKAVVVNVRAALEEARGRLQAWNGPRLEASQYVNPLSD"
     gene            53335..54807
                     /locus_tag="PK224_03925"
     CDS             53335..54807
                     /locus_tag="PK224_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14917.1"
                     /translation="MQKLKVNLEHCYGIEQLSYEFDFSTKKRFIIYAANGTMKTSFAN
                     TFERLSKGLSPEERVHGNASVCSVTDDHDVPLRPEEIFVIVPIDPEFKSEKMSTLLVK
                     KELKDRYDAIRTVLETKKTDLIKALKTLSDLPINAVEATVAKDIAFNESDFYGAMERL
                     RPNVTAKRKSKLGPVLYQSIFEERVVKLIESSDFQSKISQYMKDYEDLISGSRFFKLG
                     IFNHDNAEEIADALAKNGFFEARHSINMMARAGTDREPPIETKDKLVQVIRDEKRRIL
                     SDPKLTQTFDSINLKFTKNESTRNFRRLLESNPALIAELTNLPQLRDKLWTDYLIERT
                     EIYNDFMTVYDQTKDEIQRIFQEAREEETEWRKVLSVFRTRFSVPFDATIENQVDAML
                     HEKAPALSFTHTGKSLKTDDLWKCLSMGERRALYLLNIIFEVRARKELSHKTLYVIDD
                     IADSFDYKNKYAIVEYAKCQNSKILICFCSRTISIFTVPW"
     gene            54819..55493
                     /locus_tag="PK224_03930"
     CDS             54819..55493
                     /locus_tag="PK224_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14918.1"
                     /translation="MGENKAFVEKVTNGLKIIKDKYWSSPFLKWQEQMTENNEMLVAT
                     IPFVRNVAEFCGHHDIFDALTQFLHFKPDTHTKTVADLQDLFNKVLANPVALRDSGTS
                     VKSLIYSLAEAIITDPAEVVSLEKKIVLSIAIRMKAEEFMVGKIEDDAFWRAIQKNQT
                     VKLIKKFKAKFANDPAQKEALGVLTDVNLMTPESIHVNSFMYEPLLDMSGWHLQELYR
                     RVGRLR"
     gene            56787..58196
                     /locus_tag="PK224_03935"
     CDS             56787..58196
                     /locus_tag="PK224_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB14919.1"
                     /translation="MPNRRQRRRQKTATITNPAPNNPSSEKKPTDWRSLKLINKIVAI
                     IILLLSGGGGTYYIVSKINLTGQQQTVTNSSGVVQQQADGDIINHIYNLDSETLAKAF
                     AKSRGLQGGSEVRKHEEDLEESVDKKADEKLQQIKEAIDALKELAKASAPRKQPAIKD
                     ALEQLKDGNADLAMQTFQEVLDQQKSVGKSAYKEAAAAAMHIGHLASATNSEKAIPAF
                     RQATELEPDSSEAWNQLANALQRMDKNDEAVDALKKVLAFAQQKKDLSWQAITHHNLG
                     IVYANQGKYEDAEEMYLRALNINRELGRKSGVAYNYNSLALLNGQKGKNYIGEDYALD
                     SVNTYSEIRHLTDMEYAGLGDSLGTLAHFHAIRKDFRGAENLYCESFAIYKKLKNDYR
                     AATVYQNLGLLYQNEGQLVRAASLFKRSAVLARSVNNTGQTQRARSSLAALPADVVAK
                     AADNSEICNSSTNKKSASK"
     gene            58533..59858
                     /locus_tag="PK224_03940"
     CDS             58533..59858
                     /locus_tag="PK224_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB14920.1"
                     /translation="MDHREPTRPPFVGVAISGGGSRSANFAAAVMEELDYYGFLDHVN
                     AISAVSGGTLPAAYYVLKRKKPDWNWGNLRSRVGANIYSRLLLKHINPWKLLNYAFTE
                     YNRSDMMAEVFDDLLLDNATFGDIDPSMPQLLINATEINGASFVFSTEMSYNLRSSFA
                     DYPISRALAASTSVPGIFQGIAIRTYQTEDNFSYLHLVDGGITENLGLENLLQHYMDE
                     ENTAAHPLRKVPPRACFIFLIDASASGSEAQRVASYQRRDLRNWSDFIVDRNLMNAFD
                     VMFGRAHSQFISRLRGRLDDDAVFESPIQLNYGRVNCTIWQFHPRFLIYHGLRFDNPD
                     NYPIAAQLSAILSQIETHWKLSSPTSCSSAALQAALYDGARMLVSADRGALNTAITWF
                     ASHDLRMKQDVPRSSPYEFFSKHVSAVTSENNVYNGSGVYGQVFCERGD"
     gene            complement(60694..61263)
                     /locus_tag="PK224_03945"
     CDS             complement(60694..61263)
                     /locus_tag="PK224_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14921.1"
                     /translation="MACKGKNPPSSQNEQAKEREQSRARSKERILHATQEFQEVYSAD
                     ATWKEAFTRRPVWTIDVQARLIPNRPIIGTGYVDDVELEAGQYLIHFKRGMLEDVSAS
                     FGLGSISVSFKLRCEVFNDHRPESKRIADQVNSDHSGGLHDTHVFVAEIHSVERTYKP
                     IARDNDAELLQSRHFTATGKCLAVRYVGE"
     gene            complement(61460..61535)
                     /locus_tag="PK224_03950"
     tRNA            complement(61460..61535)
                     /locus_tag="PK224_03950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61500..61502),aa:Lys,seq:ttt)
     gene            complement(61651..62973)
                     /locus_tag="PK224_03955"
     CDS             complement(61651..62973)
                     /locus_tag="PK224_03955"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRB14922.1"
                     /translation="MANLVIIGSQWGDEGKGKIVDILAKDADMVVRYQGGSNAGHTVI
                     NKRDTFIFHLIPSGILYRGTRCLIGNGVVVDPGALIEEMDHLQGQGVKIGKNFAVSDR
                     AHLILPYHKAIDKASEQSKGVRRIGTTGRGIGPSYGDKMARIGIRMGDLLNPPLFKQK
                     LEENLVDINWLLEQLHKVDCFELEKVYQQYMGYADRLKSYIIDTALVVNKAVDAGKTV
                     LFEGAQGTHLDVDFGTYPYVTSSSSSAGGACTGTGVGPTKIDAVMGITKAYTTRVGSG
                     PFPTELTDEVGSWLQERGKEFGATTGRARRCGWFDSVIVRHATRVNGLSSLAVTKLDV
                     LDGCKELKVCTGYRVNGKIHREMPSDLLALTNCEPVYERVRGWSAPTTGVTTYKDLPA
                     EAKRYLTRIEELAECRIDMISTGSRRDETIILNNPLKAGRRVRSTRSR"
     gene            63332..63407
                     /locus_tag="PK224_03960"
     tRNA            63332..63407
                     /locus_tag="PK224_03960"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63365..63367,aa:Ala,seq:ggc)
     gene            63566..65407
                     /locus_tag="PK224_03965"
     CDS             63566..65407
                     /locus_tag="PK224_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB14923.1"
                     /translation="MLQLESVHKQFATKVLLEGATAHLRPGARVGLVGPNGAGKTTLF
                     RMILGEESPDKGSIRKRPRLRVGYLPQELETIVGKTVLDATHRDLYPEHEAERILAGL
                     GFSEADFSRPIENLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFERFLLD
                     SNLTLLVISHDTKFLDGIATHIWELRDRTIQEFRGNYTKFQELRAARDAQIESAANRQ
                     SKEVARVQNFIDRFRYQANKAKQVQSRIKQLDKVKLIERQRDTKRVRFKFPLPSASGR
                     HVLELKGVAKSYGEKVIYRSLDFTVERGQRIALVGENGAGKSTLLKILAGVLPFEKGT
                     RQVGHGVTLHYFAQHQAESLDPDDTILESLAEVSAQAETNFLRGIAGAFLFSGDDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDMLTDALTDFPGTIIFIS
                     HDPTFLTRVATLIVEVDEGQAKNYLGDYEYFLWKKAQEFESIKESSAELAAAQAGKSD
                     GPTKAMASQVQQKSQGGERRDLNKTQARLEKQVSRAESEIASMETKVKARELELADPA
                     LYQELGRWSELQREQEGWKKELERLTARWESLSDELQGVREKLTAIG"
     gene            complement(65404..65781)
                     /locus_tag="PK224_03970"
     CDS             complement(65404..65781)
                     /locus_tag="PK224_03970"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB14924.1"
                     /translation="MARQSSSAESVSERRKYVRATLVGSALVSPQSGAKGFTAVLNNV
                     NKIGAGLHSKETLPIGAKIAVSLAFLDPDRVEQQEKLNATVAWAKPWEKGILLGVVWD
                     DLVTKEKNRWLYYYLEETLKSTD"
     gene            complement(65844..67133)
                     /locus_tag="PK224_03975"
     CDS             complement(65844..67133)
                     /locus_tag="PK224_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRB14925.1"
                     /translation="MSSHDSTGDLFAPAQTATRPGKVPLAERMRPRSFDDLVGQDEVV
                     GPGRPLRNAIERDQLSSVIFWGPPGCGKTTLAGLVAQHTESQFVPFSAVTGGIPELRE
                     IIKAAEHRRAMGRATTLFVDEIHRFNKAQQDAFLPHVERGTVVLIGATTENPSFELIA
                     PLLSRSIVVLLKPLTEESLGSILDRAVADSERGLGSLRITLHPAARERLIAFGNGDAR
                     SLLTTVEFVASQAPVGPDGSRIIDERVLEAALLKKALRYDKSGEEHYNLVSAYIKSLR
                     DSDPDGALYWLARMLEGGENPRFIARRMVIFASEDIGNADPIALVMANAVAQAVEFVG
                     LPEAQINLAHGTTYLASRPKDNASYVGLQEAVRDARQHGNLGVPLHLRNAVTSLMKDI
                     GYGKGYRYVHDDPAAKTDQNHLPESLRGTRYYRPRTP"
     gene            67286..68089
                     /locus_tag="PK224_03980"
     CDS             67286..68089
                     /locus_tag="PK224_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRB14926.1"
                     /translation="MFCDTKGIRLAYDDQGTGLPLVFLHAFPLNRSMWTPQTTALSRQ
                     FRTIAVDLRGHGESDAPLWAFSLEDYADDVCALLDHLAIPQAVLVGLSMGGYVGFSFS
                     RKYGNRLKGLVLADTRAQADSPEGRTGRFNLAQTAYGKGTDAVADIMLPKLLGATSLQ
                     QKPELVDYVRRTIRHTPVSGILVDLMAMANRPDSVAHLRTLACPTLVVVGQEDHTTPL
                     ADAQVMATEIPGARLAVIPAAGHLSNLEQPEVFNDLVRNFVEELRAAGD"
     gene            complement(68086..68721)
                     /gene="folE"
                     /locus_tag="PK224_03985"
     CDS             complement(68086..68721)
                     /gene="folE"
                     /locus_tag="PK224_03985"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRB14927.1"
                     /translation="MAKVVSGARARRSRVSAAPTDEDPIEGLMTNLLLELGEDPDRNG
                     LLNTPKRVAKAMRFMTQGYRQDIDHLLNGALFPIEYDEMVIVKDIDFFSMCEHHLLPF
                     FGKCHVGYLPNKKVVGLSKIPRVVDAFSRRLQVQERLTLQIAETLKKKLNAHGVAVVM
                     EARHLCMMMRGVEKQNTVAVSSSMLGVFRTQQQTREEFLKLIRGSSVRDGN"
     gene            complement(68714..69163)
                     /locus_tag="PK224_03990"
     CDS             complement(68714..69163)
                     /locus_tag="PK224_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRB14928.1"
                     /translation="MSQATITRRYRFCAAHRLHTDQLSAEENWATFGKCNNPNGHGHN
                     YVVFVSVKGDIDPVTHQVLDVSRLDAVVERIVVQRFDHQDLNLDPEFATQTTTGENLV
                     LLIWDLLVDKLPAGRLVKVGVIETRDNYFEYSGSVESKRREEGVRHG"
     gene            complement(69296..69616)
                     /gene="erpA"
                     /locus_tag="PK224_03995"
     CDS             complement(69296..69616)
                     /gene="erpA"
                     /locus_tag="PK224_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRB14929.1"
                     /translation="MVTITQLAEQKIKELMTEEKDVVGLRIYVRGGGCHGYQYGMAFE
                     SKMADDDTVIEKGDVKVIMDSQSAPLLQGAEVDYVDSLQGSGFSIKNPQAKTTCGCGS
                     SFSA"
     gene            complement(69680..70036)
                     /locus_tag="PK224_04000"
     CDS             complement(69680..70036)
                     /locus_tag="PK224_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRB14930.1"
                     /translation="MPRVSFLHPQGRSGEVESNLTLLEAAKTLGFELNHDCGGNASCT
                     TCRVEVQMGGDNLSEIDFDEQDLLDREALSEPWHRLGCQARVLGDVVVRVPETKWVQP
                     TSASSGEVGKRGTGLP"
     gene            complement(70049..71350)
                     /gene="nuoF"
                     /locus_tag="PK224_04005"
     CDS             complement(70049..71350)
                     /gene="nuoF"
                     /locus_tag="PK224_04005"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRB14931.1"
                     /translation="MTLPQPRVLQKLEGAPWEIDPYLKTGGYEAWRKCLKELTPNDIV
                     GEIKKAGLRGRGGAGFPTGTKWDKVLNHRVQERYFVCNAGEHEPGTFKDRHLLKHIPH
                     QLIEGCLIASRTVNAKASYIYVNHEYAEEEANLRKAIAQARERGLLGKNILGTGVDLD
                     LEVYSGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDFGLYGKPTLVNNVETLCNI
                     PLILKNGSAWFTQVGTEKCPGTMLFSLSGSVNRPGVYEMPMGVTIRDLIETCGGGVPN
                     GRKIKAVFPGGPAFSMVTADQLDLPMDFDSLKKAGTGLGSAGTIVIDDATCMVAATLK
                     YSNFFKNESCGQCPPCRMGTINLATLMDKIERGEGSQKDLDSLLQLCGFVKGTGYCTL
                     ITGAAVLVQSSLRLFQHEFEEHIRLQRCPFQPARTGAGASA"
     gene            complement(71604..72191)
                     /locus_tag="PK224_04010"
     CDS             complement(71604..72191)
                     /locus_tag="PK224_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRB14932.1"
                     /translation="MKFLEDPYQTLGAGFALAVGLIVVYLGMAGVGAGDADWFGLIVR
                     WIHFLAGITWIGLLYFFNLINAGFLKSLDGPTKNIVIPKLMPAALNWFRHGATVTVLA
                     GIVLYGYLYSKGGTGALALGIGGLLGIIMMGNVHGIIWPNQKKVIAAVAAAAQGTPAP
                     PEMAQWGKTALIASRINFLLSIPMLFFMGAGSHLK"
     gene            complement(72291..73235)
                     /gene="mdh"
                     /locus_tag="PK224_04015"
     CDS             complement(72291..73235)
                     /gene="mdh"
                     /locus_tag="PK224_04015"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRB14933.1"
                     /translation="MMGRPKITVVGAGNVGGTVAQRLAERDAYDVVLVDIVPGIPQGK
                     ALDITQAGPVCGYSTRVVGSNGYDETAGSSIAVITSGIPRKPGMSRDELLATNAKIVK
                     TVVRELVSRSPNIVLILVTNPLDAMVHVARQVSGLPKSRVLGMAGVLDTARLRSFVAE
                     ELNVPGTDVQAMVLGGHGDTMVPLVRQTTVAGKPITDRLSPERLAALIKRTQDGGAEI
                     VGLLKTGSAFYAPSASAVDMVEAIMNDQKRVVPCAMLCEGEYGLKDVIVGVPVTLGRS
                     GGESIVEYELTAEERTALQASADAVRDLCAVVDRLLTA"
     gene            complement(73308..73718)
                     /locus_tag="PK224_04020"
     CDS             complement(73308..73718)
                     /locus_tag="PK224_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14934.1"
                     /translation="MIEQPPRPTIPPWLYKLFTGHQYPYVRRQAKFANRDFKPGEERP
                     EPTREQIDAKFWEIYPRCTAKILEEVKTGMIVVFHELGEYPAGGYQGLVDTPEDFLAA
                     NYGKKKIKVNFYDGDNFVCTINFKVGGWTGHEHA"
     gene            complement(73762..76797)
                     /locus_tag="PK224_04025"
     CDS             complement(73762..76797)
                     /locus_tag="PK224_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRB14935.1"
                     /translation="MAAGRSSEQLCDLLAEQLGPDTAAAVVKAIADSAARPDAVEGVL
                     VLLDELTEGAPKAAKAAIDALADMQRRGLLAEVLPWVDLGVAIAADSGALALRFFKES
                     PLLLGLLEPSSRAIVLAAGLELADRQANVAVEFIRIAPEAVGVLSPADWPVWMDLACE
                     LTETDFALAVEYIRQIPSIARVLSLDAVRAWVRFGMKLIVENSLGKPDYLGVLEFFRT
                     SPAILEDIPGPSHRNQVIGIGAQMAERSPASGIAVLAEAPAMLRRLPSEEWRTQVLRY
                     GLLVAERDAETALTYLRRCPELIALVGSGEGAEEKFQSWFASGMEVLEYSQEAGQAYF
                     AMETEKALGAVSQAMSGVPLRQIARRVKLFAQALCGRDLRIHDLPDSLESTQPMARAR
                     VSEDGQSIGLPSLVRRYPTYDENVRLYMVMVAHEAGHLEFGTFELPIVRLQDLVEDLA
                     QRYGRAVPNEARTLGDVFARYPQPGLIRDLWALVEDARVEYLLRAEYPGLSRDLASVA
                     KDAVNVRSLAHGMTVRELVVDQLLLFSTTDAGTVIVPDAVKSEVDQLWSLCRTIYHPR
                     ATAEEAVRLADRLYARMDELLAVRREAIPSQDEAEAPEQSIPAPKSSEDLSDTYRPMD
                     NWDYRGAMDPNLVRDRTESSPEPSAASGQGDSEGGAGLAGDAGGSSGAGTARSQQMSQ
                     DVLVPGRRQPSLVEEVLAVEGEGARLEDETVGAVKAVRYREWDAAIQDYRMNWCRVVE
                     REAIEGTSDFVEETLAAQRGTVTLLRRYFESLRPPGLRRISGQLDGEDLDMDAVIGRM
                     ADLSAGIEPSERIYVRREKREREVAVAFLVDLSGSTSRQVEQGRRIIDIEKQGLVLLC
                     EALTAIGDQFAVYGYSGQGRNQVDFVVVKGFDELVTGRAGAKLGGIVPLQQNRDGAAI
                     RHATSKLLARQARTRILVVISDGRPLDDGYKDDYSLEDTRMALREARASGVEPVCITI
                     DKEADPYLRRMYGDVRYVVIDRVEGLPEKLPRIYHRLTA"
     gene            76935..77381
                     /locus_tag="PK224_04030"
     CDS             76935..77381
                     /locus_tag="PK224_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HRB14936.1"
                     /translation="MAEPSKESLDKMWKYVKGFAEKSGTAMHPNEAVTNAVVLGLAAH
                     VDELGKPLCPCNFYPDKQAEAKLRRWMCACDEMQMYKYCHCLLFVREDGMPITEYLPE
                     DHEGRQCYGLITDPTPEKGRALRHKALPMAPKETTPPTTPESAPQP"
     gene            77390..78625
                     /locus_tag="PK224_04035"
     CDS             77390..78625
                     /locus_tag="PK224_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRB14937.1"
                     /translation="MPPGRNPAVALMLSALSLLLGLATTGCLRTMEAIDPRSLPITST
                     SGQRLQHHVSFLASPELSGRQPGSPGNRQAARYIEAQFRAVGLEPLPSLGGYRQAISP
                     TIGDNLLGFIPPTAGSAPAQWMVIGAHYDHLGYPFLGADDNASSVAILIETARHMAGL
                     PQHGILFVAFNSEESPYFGKAEMGSQFLFHHLPPEIGRAENLQAVVIMDLMGGVQWPP
                     IKDAIFAAGAEKSPALYQRVKQASSPSLTVFPVGMHLVEEIPEIGHKAFSDYDVFRNH
                     GLPFLFLSAGRTPRYHTAGDVTSTLHYDRMAATVEWLRHLIALIGQDEAPYGFQADRV
                     EWADEVVSFREIVNRAVQDETRIPGTSRWSLRKLRQDAEWLRDADRSAPTPQDRDRLE
                     RISIRVQCLMADYSGCFTF"
     gene            complement(78666..79118)
                     /locus_tag="PK224_04040"
     CDS             complement(78666..79118)
                     /locus_tag="PK224_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14938.1"
                     /translation="MQRGEDVKASRSSWCLMLLVVIGLTACAGPEPILKSNTRVQLYG
                     KDQAARDAAVCGEKAERAGLQHGTNRSGNAGAGATLGIVGGAAVGASAGLIGGPTGIA
                     IGAAAGGAVGGVLGFLAGTYKPLQPQPDYAAFVERCLKEKGYETTGWQ"
     gene            complement(79248..79565)
                     /locus_tag="PK224_04045"
     CDS             complement(79248..79565)
                     /locus_tag="PK224_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRB14939.1"
                     /translation="MRERQATEREQALRATSEGVTISVHVQPKASRSECAGLHGHAVK
                     IRIAAPPADGAANDELCRFLARRCDVPLSAVHILSGAGSRQKRVLVRGRSVEQVRAQL
                     HLE"
     gene            complement(79631..80470)
                     /locus_tag="PK224_04050"
     CDS             complement(79631..80470)
                     /locus_tag="PK224_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HRB14940.1"
                     /translation="MSEGRTASTPKGQEQEIDRYRIAREPFYAEVRGEVGLFTIAAQS
                     RMPVMLKGPTGCGKTRFVQHMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWMD
                     GPLTLGVKQGAIVYLDEIVEARKDTTVIIHPLSDDRRLLPIEKKGQVIEAVDDFMLVI
                     SYNPGYQSILKDLKQSTKQRFMAIEFDYPLPDVESRVVAHEAGVSLEVAQRLVKIAEK
                     VRNLKNHGLEEGVSTRLLIYAATLIRQGVPADQACDVAIARPITDDPDMLRSIIEVVK
                     AIF"
     gene            complement(80591..83692)
                     /locus_tag="PK224_04055"
     CDS             complement(80591..83692)
                     /locus_tag="PK224_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB14941.1"
                     /translation="MAASLPAGSPRRSYPWLPVLIVGVTIVALVIGVVMLRSIEVRMV
                     EATGENLTLASAEIADKLDRLLFERHADVRMMARAFSDRALDKKYINEYLQWMKETYA
                     PVYLWLGVTDAHGRMVAATDSSVIGQDYSRSGWFDRVRQTGTVQVDEVEIHESNHKIE
                     SVAFTAPILSVGGQFLGAVTTRIGLPMLEDVLIETVREIQQTDGSAGPFEYQFLTKAG
                     VVFVDSASSGIDRVNLTELGLPSVLLSRSGKAGYVEEMHVRRHVPVVTGYSQTKGYSD
                     YSGLQWTILLRMDRDLILLPIRAMMWKLGMAGVVVWLPMVGLLLWATGRLREQHRQAQ
                     QESEWARAAEAALLQSQERNRVIVDTALDAVISMDAGGIITDWNAQAVNIFGWEREEA
                     LGRRVSETVIPVRDRDAHERGLRHFLDTGQGALLNRRIEMMGSHRDGHEFPVEITISP
                     ARLGDAYIFSAFIRDITARKRTERQLASQYAVTRVLAESRTLEEAGPKILQAICESLE
                     WELGIFWRLDRQGSVLRCLDVWQSPALNAEEFVLDTWQHTFTIGKGLPGRIWQAGEPT
                     WIKDVVQEANFPRGGAAARVGLHGAFGFPVRVGTEVEGVIELFRREIKQPDEQLLKMV
                     ADVGLKIGQFGERARAEDALRRTEAQLQQSQKMEAVGRLAGGVAHDFNNMLTVIRGYS
                     ELVLSRLAPTDGFRKELEEVKKAADRASGLTGQLLAFSRRQFIAPKVLDLNSVIHNME
                     GMLRRLLGEDIVELCTVLDPGLGQLKADPGQVEQVIMNLAVNARDAMPNGGRLTVETS
                     NVQFGQRRNRPPMGAEPGSYVALIVRDTGHGMDEDTQSHVFEPFFTTKEKGKGTGLGL
                     STVYGIVKQSGGCIEVESKPGRGATFKIYFPRVESAATETESVAVGGDSVRGRETVLL
                     VEDEPGVRRLVNETLRLHGYTVLEARHGIEALLTGAKHAGPIHLLLTDVVMPQMSGPE
                     VAEKLATVRPEVKVLYMSGYPDHPAFSKGGIDTEHSFLQKPFTPTTLAQKVREVLDGS
                     KVA"
     gene            complement(83695..85182)
                     /locus_tag="PK224_04060"
     CDS             complement(83695..85182)
                     /locus_tag="PK224_04060"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRB14942.1"
                     /translation="MPIVPTNPSRPGGDALLQIVPSSACLSCDVCCRFPERDSFLRPF
                     FTADEIGTAIAAGLAPALFPNPDGSQIDLVPNPEGDGYVCPAFDCATSRCRIYEVRPL
                     DCRLYPFALMWDARHEQVLLGWDTKCPYMRDQSSPLVAQAADEISGLIHQDPRIDKHV
                     RYPRLIGRFQEDVIVLRPLERVTERLQQGRMRVRTQAFTLGDRGRMDAALAVSAGVQG
                     TPLAAASFAYHYIWRHRVTYSWAVLHDHLCLFADCPDGLFMALPPLGPGPLAQPLAEA
                     LRFMRERNGDSRVTRVENVPEACVDEVRALGYRATAKEPDYLYDASALSDLSGESFKS
                     PRAACNRFIRERGGMLEPYEPRDERACVSLFHEWREQKQQAGSDDWACALLEDAAGAH
                     ETALSDAAELGLIGAVVRVGGRIRAYTMGLWLNPSVFCVLLEVADRDIVGLGPFVFRE
                     FCRQARAKGACWINTMDDSGLPSLARSKQWYHPARLLPNYIVTES"
     gene            85425..86939
                     /locus_tag="PK224_04065"
     CDS             85425..86939
                     /locus_tag="PK224_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14943.1"
                     /translation="MALAQHSREPLNSDEARRRCLAIRDVLVRAGVYGPAETGDGAAT
                     TDSSIPTPRFRLAPTPFPLSSSDVTFFQELGQDLLSFYRALNRLYQDSTRGTQPAWVA
                     AYLDQGKPESLITYSRMNRFRTLIPGIIRPDVIPTADGMVITELDSVPGGIGLTGCLT
                     RAYDDQFTIECQPPGLPPERSPLIAGRDGMLQGFAAMLRAQQGDQEGCLAIVVSDEAK
                     EYRAEMEWMAQRLREIGYQAFCVEPRAVHFSEEGLFLQEETGPRPIGLLYRFFELFDL
                     KNIPKAELVMYAAKKGQVTVTPPYKPALEEKLAFGLFHHPELAPFWEQALRPETAINL
                     RRLLPRTWILDPQPVPPSAVIPHLTISGRAVSDFRRLAQTTQKERQLVLKPSGFSELA
                     WGSRGVSIGHDLPQVEWATALEQALQAFPTTPYVLQEFHKGRLFELSYFDERSDTVVP
                     MSGRVRLSPYYFVVGDKAELGGILATLCPANKKIIHGMVDAIMAPCAIAPNLAS"
     gene            87015..87260
                     /locus_tag="PK224_04070"
     CDS             87015..87260
                     /locus_tag="PK224_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRB14944.1"
                     /translation="MALTLYHVQWCPDCAVVRDRLDELKLSYEDVVVPDFRPMRTQVF
                     EVSGQYYVPVLKDGDTVLTETHDILAHLHTQYDKARP"
     gene            87570..88703
                     /locus_tag="PK224_04075"
     CDS             87570..88703
                     /locus_tag="PK224_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRB14945.1"
                     /translation="MEDWRRILAQSVVKPKDLADRLGVDPKEIEDIVGDYPMRITPTV
                     LATIKEKGDAIWKQVVPDRAEMADADAEDDPLEEDLMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDRAIAYLRDHQEVRDVILSGGDPLLLPDHLLER
                     ILKSLRTIPHLELIRIGTRVPGSLPERITPKLCEIIKKYHPFYMNLHFNHPDELTPEV
                     KRACGMLADAGVPLGAQTVLLKGVNDDPEIMKRLMHQLLLARVKPYYLYQADLTKGTN
                     HFRTSVETGLKIIKSLQGHTSGMGVPHFVIDAPGGGGKIPLLPADYLVNLDEDSAVLR
                     NYENRTFHYPQPSSTPGRELPMVGAHPSWASTGNGCDGGGEHL"
     gene            88700..89851
                     /locus_tag="PK224_04080"
     CDS             88700..89851
                     /locus_tag="PK224_04080"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HRB14946.1"
                     /translation="MTRAASSKGILPYQHITQLIARGSITSDHPIEDRQIQPASLDLR
                     LGKKAYRLISSFLPELSAISSRLNVLDFYQSDLVMYEMDLTQGAILEKGHVYLVPLLE
                     QLDLPATLRARANPKSTTGRLDVFTRVVTDLNAGFDEIRAGYRGPLYLEVVPRSFAIK
                     VRTGDSLNQIRFVRGDATVSDSALQKLHGTDPLLFRNASPPKPLPQKEFRTERGLFLR
                     IDLTGSAREDAVIGYRAKKNSHVIDLAKIGHYAALDFWEPLKRHRQDSLLLEPEEFYI
                     LASKERIRVPPGYAAEMVAYEAACGELRTHYAGFFDPGFGYGDGKMRGTQVVLEVRPH
                     DVPFLIHDGQTFFKVVYDRMLTVPTQVYGTALGSSYQRQALTLSKHFKA"
     gene            89854..90261
                     /locus_tag="PK224_04085"
     CDS             89854..90261
                     /locus_tag="PK224_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14947.1"
                     /translation="MAPPSDLPDPSTRPSRPPLHRPDDESNVDRQTHVVGMMVGTALM
                     FIGFLDVFLSISGGFEINVVPLLIYFSGIAVWANAVVENPTIRYSLMAGSVLISLAFF
                     HYGEVLFWHKQVVFWSTVVVVMYFMFNEPKQAT"
     gene            90326..91054
                     /locus_tag="PK224_04090"
     CDS             90326..91054
                     /locus_tag="PK224_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRB14948.1"
                     /translation="MTIAVIIPVLNEAFGIGQTLSHTATLGFDELIIVDGGSSDQTCA
                     VVESCAGRLQNRPYSGPAPASPAIRLLKAAAGRARQLNAGAAGSRCEVLLFLHADTYL
                     PTNARQAISTALSDEACVGGRFDVRFDSPRPIARLVGHMMNLRSRWSGIATGDQALFV
                     RRDVFERTGRFAEIPLMEDIEFSRRLKRAGRLAPLRNQVVTAFRRWERNGPVRTILLM
                     WTLRFLYWIGVSPYRLQHFYSIVR"
     gene            91059..91811
                     /locus_tag="PK224_04095"
     CDS             91059..91811
                     /locus_tag="PK224_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRB14949.1"
                     /translation="MSAPPSDRKQPASAALVIFAKAPIPGQVKTRLCPGLTEDEAATL
                     HGSFVLDTLERTKAAVHKFKLPVDRFLACAPSSTHVFFRIMEARHGVTLLDQPGDNLG
                     DRMAQAFSTLFARGYRHVFLVGTDVPSLPLTHYRDAVQSLTRHDLILGPAKDGGYYLI
                     GMTAPHPDLFSDIPWSTDQVMGLTQQKAAAAGLKTALLPAWSDVDTLDDLHALIDDCA
                     ADKKRPKQERVYSQRTAGTLELLAKRLRTRHT"
     gene            91822..92556
                     /locus_tag="PK224_04100"
     CDS             91822..92556
                     /locus_tag="PK224_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB14950.1"
                     /translation="MPNKVALITGGAKGIGRAIALDLAAQQWSVAICYRTSKAAADET
                     SAAIRARGGQALTVQCDVSDPKAAGDLVSQVESRWGRIDALINGAGPYHRVNLFDETP
                     AGWREMFEGNLHPIFYLGQAVAPGMKARKQGRIINFSMANADQMVAQPEVTGHYIAKA
                     GVLILTRTLAKLLAPYGITVNAISPGFIDSGSAPPDELAGVVKRIPAGYIGSVGDTVA
                     AVRYLLSEEARYVNGANLHLSGGWGI"
     gene            complement(92566..>93012)
                     /locus_tag="PK224_04105"
     CDS             complement(92566..>93012)
                     /locus_tag="PK224_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14951.1"
                     /translation="VRVYGGGAATGVAPSHWLLTFSLFLFLSLALVKRFTELRLTGEA
                     EGKELHGRGYWVTDLEHVSTIGTASGLLAVLVLALYISSKEVLLLYTHADVLWLVCPV
                     MLYWISRVWMLAYRNRMDDDPVVFAVKDSKSYLMAAIIGAILFFAR"
BASE COUNT        21013 a        27091 c        25190 g        19718 t
ORIGIN      
        1 accggcagcc aaacgaacgg cctgatccga tcgtctcttc ccgtcggtca ccaggaccga
       61 accgctcaac acggtgactt gtacgtccgt cttgcgatcc ttgacaaaaa attgggtgcc
      121 gacggccgtc gtggcgattc cgcggctctc aacggtaaac ggccgttccg gattcggctg
      181 aacggcaaag gacgcctcgc ctcgcaacaa ttccacttgc cgacgatcgg actcatacaa
      241 caccgcgatc gaggtgtccg tatttaaggt gacgatcgat cgatccggaa gggtcacgac
      301 ccgctgttct ccgattcccg tcgaatagtc ggatttgagc cacatcaata ccgcatcgct
      361 ccagagagcc atgccgacga gcaagaagac ggctgcggcc gccatgcttc ccctgcgcca
      421 ggaatgatgc gcctgccggc gcaacgcatc gatcgtctcg ctcgtcacgc tccgggccgc
      481 agcctgctgc aattccggcg cattccacag cgcctgaacc tggtggtagg cgcgcgcatg
      541 cgacggactt tgcgcgcgcc acgcgtctaa gcggcgtcgc tcggcaccgg aaaaatcgtc
      601 ggcggcctgg cgcataaacc aggcggtcgc ttctttcatc agttgcgaat cgggtagttg
      661 gggcacggac ggccccggcg aacgattgtc ttcaggtcgc attgtggccc atggcggctc
      721 ggcaaactgg gaacatgaga gcgatcgacg cactatagtc tcttatgccc gacgtgtcag
      781 ctcgctcttt tccccctcac acccatcagg ccggcaggtc tttggagagc agcgcttcct
      841 ggcagcaggt catcgctatg acgagctgtt tttcgactgc gcgttccgac atccccaatt
      901 gcgccgcaat ttcctggtag gaatacccgt acactcgccg catggcaaag acagcccggg
      961 tgcggttcgg gagggcggac agcgccttct caagcgtagc gaccaattgc ttgtcatgcg
     1021 cctgggcatc gatcggtcgc gtgtcggtgg gaacctgttc ggcttcttca agcgacgttt
     1081 gccgctgacc gcggaatttt tgttttctaa aatggtccag agcgagattc gtggcggtcc
     1141 gaaacaagaa tgcgcgaggg gacgcaatga tctgcgatgt ggggacatcg gcgagacgaa
     1201 tatacgcctc ctgagccagg tcggctgccg tttcctgaca tcccaccatg cgccggagca
     1261 tggccaggag atcgcgctga tgttcgtgaa acaaccgttc cagatcgatc gaaggtgagg
     1321 cgctcatgtc atcgttgctc agggccagtc ggcgcggcgg tcgtgcacac atccgctgca
     1381 ggcaatgccg ggatcttgtg acttctgagg gagcagccta gagtttcgca aacgaaaaga
     1441 gaatgatcgg attccggata gtgcaggcga gaaagtgatt gtgaggatta agccggatga
     1501 gatcggttat cggcccgtta tgcggcaggg tgcagagcgg ccgcccgggc tcgtacccat
     1561 cgaagcctca ggcgatcgag gaagaggtag acgacgggag tcgtatagag ggtaaggacc
     1621 tggctcacga gcaggccgcc gacgatcgca atgccgagcg gacgccgcaa ctccgatccc
     1681 acccccatgc ccaccgccag cggaagcgcg cccaacaagg ccgccgccgt ggtcatcatg
     1741 atcggacgga accgcaacag gcaggcttcg tagatcgctt cctgcggagt cttcccttcc
     1801 gccatccgtt cgctgtgcaa cgcgaagtcg atcatcatga tggcgttttt tttcacgatg
     1861 ccgatcagca acatgatccc gatcaacgcg atcatgctca gctccgtctt gaacaggagc
     1921 agggccagca acgcgccgac gccggctgag ggcagcgtcg agagaatcgt gatcgggtgg
     1981 atgtagcttt catagagaat tcccagcacg atatacaccg tgaccaacgc cgccaggatc
     2041 agccagggct gattctcgac ggacgactga aaggccttcg ccgtgccttg aaaagtgccg
     2101 cgcacagtcg cgggcaggcc gatgtcatgc atcgcctgat cgacagcgag cacagcctgc
     2161 ccgagcgatg cgcccggcgc catgttgaac gacaccgtca cggcgggaaa ctgcccctgg
     2221 tgattcaccg agaggatggt gttggccggc tcgtatcgcg tcacggcact caacggcact
     2281 tgcgcgccgt tcggcgagcg gacgtaaata tcgtggagcg cagaggggtt ctgccaatat
     2341 tgcggcgcca cctccatcac cacatggtac tgattgagcg gcgtatacat aatcgagacc
     2401 tgccgctggc cgaaggcatc atacagcgtg tcgtcgatca attgaggact caagccgagc
     2461 cgtgacgccg tgacgcgatc gaacaccacc agcgattgct gtccccgatc ctgctgatcg
     2521 ctgttcacat ccacgatctc ctggatagtg cgcaacttgg cctcgacctt cggagcccag
     2581 gtgttgagtt cggccagatc cacactctgc agggtatatt gatattgtgc gctgctcgcc
     2641 cgaccgccga tacgcaaatc ctgaatcgcc tgtaaatagg tcggcgcgcc cggcaccttc
     2701 gccagcttgg ggcgtaaccg ggcaatcacc tcgtccgaac tgaggccgcg ttcttccagc
     2761 ggcttcagcg caatgaacat ccggcccgta ttggtggtac ccgcatggcc gccgcctccg
     2821 gtaaacccgg tgatactcgc gacggcggga tcgctcttga tgatgtccac cacgtcagtc
     2881 aatttctgac gcatcgcctg aaacgagata tcctgtgcgg cctgaatgtt tccaaagagc
     2941 cgccccgtat cctgctgagg gaaaaagcct ttcggcacga ggatgtacag atacaccgtg
     3001 agcgccatgg tgcccaatgt caccaccaac attgtgcgcg ggtggcggag aacccagcgt
     3061 aggctggccg cgtatccacc ggccaacagg ccgaaccccc gctcactggt gcgataccac
     3121 cagccatggg actctgccgg ttctgacttc aacacccgcg cgcagagcat cgggatggtc
     3181 gtaagcgaca ccagcaacga caccagaata gcggtcgaga gcgtcaccgc aaattcacga
     3241 aacagccggc cgagcatgcc gcccatcaag aagatcggga tgaacaccgc caccagcgag
     3301 agggtcatcg acacgaccgt aaacgcgatc tccttcgcgc cgcggagcgc cgcgtccacc
     3361 ggcgacaacc cctgttcccg gtagcgggtg atgttctcga gcaccacgat cgcatcgtcc
     3421 accacaaagc cggtggcgat cgtgagcgcc atgagggaaa gattgtcgag gctatacccg
     3481 aacaggtaca tcacgccaaa cgtggagatc agcgacacag gcaccgccac cgccgggatc
     3541 aaggtggcgc gcagattccg gagaaacacg aacaccacca gaatgaccag tccgacggaa
     3601 atggccatgg tacgctcgac gtcgtgcagc gaggcgcgga tgacgggcgt acgatccatg
     3661 acgatcgaca gcgacatact ccccggcagc gaggcctcca attgagggag ttgcgcacgc
     3721 agccgatcca ccgtttcgat gatgttggca cccgcttggc gataaataat caccagcacc
     3781 gccggggtgc cgttggccac ccccatggtc cggagatctt cgacggacgt ttccaccgag
     3841 gctacatcgg acagttgcac cgcccgcccg tttcgatagg ccacgatcaa cggcagatac
     3901 tcctccgcct gctgaagttg gtcgttggtg cggatttccc aggtgcggtc gtcgcttgtc
     3961 agttgccctt tcggacgatt cacattcgta ctggtcagga cctggcgcac gtctcccagt
     4021 ccaatgccgt acttattcaa agcggtcgga ttgagatcca cccggatcgc cggcagagac
     4081 ccgcctccca cggcgacctg cccgacgcct tcgacctgcg acagcttctg ctgcaggatg
     4141 ctggaggcgg catcgtacat ctgcccgcga ctcacgagtt cggacgtcaa agcgaggatg
     4201 agaatggggc cgtcggccgg attcaccttg ctgtaccgtg gaacagtcgg cagattcgac
     4261 ggcagatcgg cgcgcgcagc attgatggcc gcttgcacgt cccgcgctgc cccgtcgata
     4321 tcgcggctca gctcgaattg aagcgtgacg ttggtcccgc cgatcgtgct ggaagaagtc
     4381 atctccgtca cgcccgcgat acgggtgaac tggcgctcca acggcgcggc gacggacgag
     4441 gccatcgtct cggggctcgc ccccggcagc gaggccgaca cattgatcgt aggaaattcg
     4501 acttgcggga gagaggcaac aggcaggaat tgaaacgcga cgatgcccac cagcgtcgca
     4561 ccgatggtga gcaacgtcgt ggcgaccggc cgtcgtacga agggtgctga caggttcatc
     4621 tcggacctgc cgggaccgtc tcaggcatcg gcatctgcac agagtcgcgc cgaaaccggg
     4681 ccgccaatcg atccagggca agatacacga ccggggtcgt gtagagagtc agcaactggc
     4741 tcaccaccaa tccaccgacg atggcaattc caagtggttg ccgcaactcc gagccgaccc
     4801 ccgaacccat cgccagtggc aacgccccga agagcgccgc catggtcgtc atcatgatcg
     4861 ggcgaaaacg caggagtccg gcttcgtaaa tggcctgttc cggcggcttg ccctcgttgc
     4921 gttccgcctc caaggcgaaa tcgatcatca tgatggcatt cttcttcacg atgccgatca
     4981 ggagaatgat gccgatcagc gcgatgatgc tgaactccat acggaacaac atcagggcca
     5041 acagcgcgcc gactcccgca gacggcaacg tggacaggat cgtgagtgga tgaatgtaac
     5101 tctcatacaa aatcccgagc acgatataga cggtgatcag ggcggccagg atcagcaggg
     5161 gttcatgttc taaggagttt tgaaaggctt gggcggcgcc ctggaaactc ccgcggatgc
     5221 tcgggggaag ccccagttcc tgcgtcactt gccggatcgc cgtcaccgct tcaccgaggg
     5281 accctcccgg cgcaaggttg aacgagatcg tcacggcagg aaactggccc tgtcggctga
     5341 tgaccaacgg caccatggtt tcggtgaccc tcgtgaagac gttcaagggc accgacccgc
     5401 cgcttccgcg cacttccaac tgctgcagcg cctccggccc cttttgaaac tccggcatca
     5461 cctcaagaat gacgcggtac tggttcagtt gcgtgaacat ggtggagacc tgccgctgac
     5521 cgaaggcatc gtagagtgta tcggtgatga gttgcggcgt gatacccata cgggaggcgg
     5581 tattgcggtc gatctccacc agcgacgcca gcccctgatt cagttgatcg ctgctgacat
     5641 cccgaagctc cggccgtacc tgcaggctct ccagcagttt cggagcccag cgattcagtt
     5701 cctcaggatc ggcgtcttcc agcgtatatt gaaactgggt gcggctgacc cgatcttcca
     5761 cggtgagatc ctgcaccggc tgcatgtgca gggtgatccc atccacctcc gccacgcgtg
     5821 cttcgatccg ccggatgatc tcgcccgccc ccgccatccg ttcccccaaa ggcttcagat
     5881 tgatatacat ccgcccgctg ttgagcgtgc tgttcgtccc atcgaccccg atgaaggagg
     5941 agaggctctc cacggccggc tcttcgagaa tcacacgcgc caacgcctgt tgccgctctc
     6001 ccatggtgcc gaaagacacc gcttgcggtc cttccgtgat ccccagaatg acaccggtgt
     6061 cctgaagcgg aaagaagccc ttgggaataa gcaggaagag cagcagggta agcgccagag
     6121 tgccgcccgt cacgaccaac gtggcgcgct gacgacggag cacccactgc aaacttctcc
     6181 catacccggc aatgatgcga tccaacaccc gctgcgacgc ctgcgaaaac cggcctggct
     6241 gttcgttccg ccgctcgcgc aacagccggg cgcacatcat cggcgtcagg gtcaacgaca
     6301 cgaccgcgga gatgaggatg gtgatgctca gcgtgacggc aaattcacgg aacaggcgcc
     6361 cgaccacatc gcccatgaac aacaagggaa tgagcacagc gatcagagac accgtcagcg
     6421 acaggatggt aaaggcgatc tgcttcgatc ctttgagcgc cgcctgaagc ggcgactccc
     6481 cctgttcgat gtaccgcgcg atgttctcga tcatcacgat ggcatcgtcc accacgaagc
     6541 cggtcgagat ggtcaatgcc atcaacgtca ggttattcaa actgaagccc atcaggtaca
     6601 tgacaccgaa cgtgccgatc agcgacaagg gcaccgccac cgccggaatc accgtggccg
     6661 gtaacgtccg gagaaacagg aagatcacca ggatgacgag cgccaccgcg agcaccagtt
     6721 cgaactgcac atcgcggacg gtcgcgcgga tcgaggtggt gcggtcggtc aacaccgaga
     6781 cctgcacgga ggaaggcaag gtcccttgca attgcggcaa cagtcgctta acccgatcaa
     6841 cgacttcgat gacattcgtc cccggctggc gctggatgtt gacgatcacg gccggtgtct
     6901 cattcatcca tgccgcctgc ttggtattct cgacatcgtc gatcacctcg gcgacctcgg
     6961 ataaccggac cggagctccg ttgcgatagg cgacgatcaa cgctttgtaa tctttgccgg
     7021 caaacaattg gtcggtggcg ttgatgatgg acgcgcgccg gggtccgtcg aatccgcctt
     7081 tggcttgatt gacgttcgcg gcggccacga cggttcgaag atcctccaac gtcaatccat
     7141 aggcagccaa ggcgcgggga ttggcttgaa tgcgcacggc gggccgctgt ccgccgctaa
     7201 tactgaccag gccgacgccg gataattgtg aaatcttttg cgcgaaccgg gtttccgcga
     7261 aatcacgcac ttgaggaaga ggcagcgtgg ccgaggtcaa tgccagggtg agaatcggcg
     7321 catcggccgg gttgaccttg ctgtagaccg gaggagccgg cagatcgcgc ggcaggaaag
     7381 tcgccgccgc attcatcgcc gcctgcactt cctgctcggc cacatccaga tcgatatcga
     7441 ggctgaattg cagggtgacg accgaactgc cgcctgagct cgtggaggtc atctggttca
     7501 gtccgggcat ttgcccgaac tgccgttcca gcggcgcggt gatcgacgag gtcatgacat
     7561 ccggtccggc gccgggataa aacgtcatca cctgaatggt cgggtaatcg acttgcggaa
     7621 gggcggagac aggcaactgg cggtaggcca gcgccccggc cagaagaatg gcgatcatcg
     7681 tcaggaccgt cgccacaggc cggacgatga acagccgcga cggattcacg tccgcttccc
     7741 tcgtgtgcgc ccctcaccgc cgggcgccgg cgcctcctga ctggccggga ccgctccggc
     7801 tgccggacgc agctcgacct tgctgccttc gcgcaacttt tcggtcccgt ccacgaccac
     7861 cacctcgcct gaggtcaaac ccgtgtcgat ggcggcttct tctccctgcg cgacacccac
     7921 cgtcacggct cgcacgctga ccgtttgctc cgcctcgttc accacataga caaacgtgcc
     7981 cttcggcccg cgctgaacgg ctgcagacgg caccaccgtg acaccctgct tgatatccag
     8041 gagcaaccgg gcattcacaa actgattggg aaacagcgag ctgtcatcgt tcggaaacac
     8101 cgctttcagg cggacggtgc cggtggtcgg atcgatctga ttatcgacgg tgagcagcgt
     8161 tcccgtggcc aacttccgct tttgctcccg gtcgaaggcc tccacgacca attgcttccc
     8221 ggccttgagc cgctcgaaga cagacggcaa atggtcctcc gccagggcga acacgactgt
     8281 gatgggctgc aattgcgtga tcaccaacaa cccgttggga tcgttcgcat ggacgatatt
     8341 gccggcatcc accaggcgca accccacccg cccgttgatc ggcgcggtaa tgctggagta
     8401 ggtcaattgc agcttggcat tgtcgatctg gccctgatcg gccttgacga tgccctcgta
     8461 ctgccgcacc agcgcttcct gcgtgtcgag ctgctgcttg ggaatgaagt tttgtttata
     8521 gagatcccgg tagcgctcca gatccagctg cgcatttttc atttgcgcct gatcgcgcgc
     8581 catttgtcct tcggcctgaa tcagctgcac ctcgaacggc cgcggatcga tctgcgccag
     8641 taattcgccg ctatgcacga gctgcccttc cttgaagagc acccgcatca actggccgtc
     8701 cacccggctc ttcaccgtga cggtgttcat cgggatcacc gaacccagac cgttcaggta
     8761 gatatggatt tcgctggttt tggctgccac cgccacgacc ggcgtgctcc gggcccccat
     8821 ggctggccgc ttctccgcct ggcgtggctg cgcttcaccg gatcgggtca tcaacacata
     8881 gacccccacc gccagcaggc aggccgcgca cagcccgatc atccatcgct tcaccagggt
     8941 tgcaaatcgc acggattcag ccatcgccgc tattccttcc gtcccttggt cacctgcgcc
     9001 tgctgcgtca cctgctcacc gcccacgctc gcgaacagcc aggtcgtcgt tccatcgtcg
     9061 cgcacagaca tgagcccttc tcatctgaga cgtttcatgc cggctgattt ccccccgcta
     9121 tcatacccta tctgaccgcg ggaccacaga tattgcgcct cgacgcccct gccggcaaga
     9181 cccgggggcc acgtgtccac gcacatgaac aggtgataca ggcacatgat aggcagtccg
     9241 taccgggagg ctgatttcag gcggcctgaa gccgacgccc cgccgaagat gacaaggccc
     9301 gcgagtcaga gggtccggcg aacgcgctca cgaacgccaa tgttcagcca caacggcccg
     9361 cacctgatcg gtcagcgcct tggccgcccc gctcctgtag ccgccctttt tccataagag
     9421 cccaatgcct cgacgagggg tggggttctt cagcgcgatg gcccgaagcg tctggttacg
     9481 cgccaggccg agagataagc gcggcaagac cgtggcgagg gtgctcctgc gaacagtcgc
     9541 cagaatcccc tcgatggaat tcatttccac gataatcttg ggctggacac cggcctgctc
     9601 gaaacaccca tccaacaagc gtcgggtgca gaacatattc ggcagcagaa tcaacgactc
     9661 ctgcgccagg gccgacaggg acaggtgccg gcgcccggca aaccggtgcc tgggggaggc
     9721 gatcaacaca aagtcttctt caaacagcgg ctggctctcg atgcggtccg acaccaccgg
     9781 cacgaacccg atagcgactt ccagcgaccc gctcttgacg ccggcctcga tgtccggacc
     9841 ggacagttcg tcgagcttga gcgacaccag cggatgcagg gtcgagaagc ggccgacgat
     9901 ttcgggaatc agataggcgt tcacggtttg aacgacaccg accgtgaggg tcccccgctg
     9961 gagcccctct tcctcggcaa tcgccacttg cgccaattcc atctcccgca aggcccgccg
    10021 ggcatgctcg cgaaaaatcg atccggcccg cgtcaattga accgatcgcc ccacgcgatc
    10081 gaacagcggc acccccactt cctgctccag ctgcttgatc tgcgcggaca acgccggctg
    10141 ggaaaccggc agcctttcgg cggccttggt gaaatgcagc gcatcggcca cggcgagaaa
    10201 ataccggaga tggcgaaatt ccatgccgcg ttttatcata aatggaagtg atgatactaa
    10261 tcagaacaaa cgctttgacg ccttctcgtc ccgggcacta ggttgactgc ggccgggaaa
    10321 agcggaaaac tcgtggcgga agggacgggg ctgtgcaaaa cacaagcggg ctcatacgat
    10381 cgatccaggt ggggctcccg tccagtgagc acagcgactg tgcggacgat ccggactcaa
    10441 cgccctggac gaccggaatg ttcaagcgct ccaccccggg ccagatccgg cttggccggc
    10501 acaatctggc aggagatgga caggcggatc tcgtccatca tggaggcgtc gacaaggcgg
    10561 tctgtgccta tccgtcggag cactggttgt attggcgcgg gatcctgccg ccccatcgtc
    10621 tcaccggcgg cgcattcggc gagaatttca cgctggaggg gctcacggag gccgatgtct
    10681 gcatcggcga tgtcttttca gcgggtacgg ccgtcgtgca gatttctcaa cccagacagc
    10741 cctgctggaa actcgcccgt cgctggcaga tcaaagatct ggcggtacaa atggaacgca
    10801 ccggcttcac aggctggtat ttccgggtca tccaggaagg tacggtcgaa ccgggcgcgc
    10861 ccctgcagct catggaacgc ccctgcccag aatggacggt cgcgaccgcc aatcggatca
    10921 tgcaccatga acggaacaat ctggccgcgg ccgaacacct gagcctctgc ccgttacttt
    10981 ccgcaagctg gcaacagacc ttgcggcagc gatgccacaa tcaggcaggg cctgacccgc
    11041 agcatcgacg attcggcacg actcattctt cgtcatcatc attctgagga ggtttccatg
    11101 aagcgattgg ttctctccgc ctgtgcactg accatcggcg ccaccctcgc cctgaattcc
    11161 tggtgtgccg ctgaaaatac gactaccatc aatatcctct ccccgcacaa tggcgagaag
    11221 gtctcggaga ccttcgacat caagtatgaa atcctgaacg cgccggcagg caatcacgcg
    11281 cacgtctacc tcgacggcgc gtaccagaag ggcttcaagg gcacgtttac acgcgtggcc
    11341 aagggggagc atacgattac ggtcaagatc gccgaccacg atcataagga cgccggaacc
    11401 gctacggata gcattaccgt gaaggtggga gagtaggacc ggggtgcgtc acgagaaaag
    11461 cgatcgggga ggccgcaacg atgccgcctc cccgccaata gcctacctgc ctattgccca
    11521 ccttgggtcg acgcacgcgt cgagctgccc tgtagatcca tatccagcaa gacaggaccg
    11581 ttcggattct ttgcgtcgag ccggatcggc atccggtctg ccaccagctt gaccagcggc
    11641 acagccacga tatgtggcgc cttttccgac ggattggaat agagcgcgat acggacattc
    11701 ttcgggcgtt ggctcggcgc cacctgagtc atcagttccg cgcccatgaa tccgaccaac
    11761 attccactgc catcggcaag cttgtgaatc tcgtaggcat tttcggcggg tgccacccga
    11821 gcgacggcga gatccggact ctgcgtcact aaggccaggc ctaggagctt cggcacaatg
    11881 aatccaaacg tccccgtcgg cgcttgcgaa aacatcactt gcgcggtcga agccccgagt
    11941 tcagccaccc cgttcttggc gcgcatctgg cgtcgcaact ccgcttcatt gccgagcccc
    12001 tcaatgatca ccgccgccgg cgcggctttt tgcaccggca acacctcggt cttgccgcca
    12061 ccgcgggcgg cgttgagggg ggacggggcc agaccgatga acagcaggcc gaccaggctt
    12121 gcagccacga ggtgacggag cgggatgtga ggagacgatc ggacaatgaa gtgaagcatg
    12181 gatgctggtc ctcctgatca agcgatgtgt gtgcgctcgg aggattctaa gaagctgccg
    12241 gtggaaaatc aaacaggaag gacgtgaacg ggcagacctg gcgagccgca acgggaccct
    12301 ccccctcgcc agaccgtacc tcacggataa aaactgatcc gtcgtccggc tatagcctgc
    12361 gccacagacg tgagaatctg ctcccgttcc gccggtttct ccaaacactc caccacacct
    12421 tgcttgcgta gcgaactggc caactgctca tcgtgatacc ccgtcaacac gatgaccggg
    12481 agggaaggat attgggcgcg gaaataggca atggcctcga ccccgttgat acgcggcatc
    12541 cggatatcgc aaatgatgac atcgaccatc aacggattgt cgccgctgtt caagacctca
    12601 accgccctgc cgccgtcctc cgcctcctcg acgtcgtatc ccgcctgtgt cagcgtcatg
    12661 cgtagcaggc tgcgaatgga atcctcatcg tccaccacca gtacccgccc tagacagacc
    12721 ttctcgtctg caaccaatcc gaacggccat gccattccca tggtgacctc ccctcgccct
    12781 tcgcaacggg ttttctcaca ccattcaatc gtgcaaagaa tgcaaggagg acgcctgcag
    12841 cgtggggtga gcagggtcac gccaaatcgc ctggttcgcc ttggatggat agccggacat
    12901 ccggggatcg atgaaatcgg gtggtggaac cccaccacca gcggtgcaaa agcaccatca
    12961 ttccgtggag atgacgttga gggcgcaaag aggctgtcct cacgatccgt cgttcgtgaa
    13021 acgtcgctgg tctctcgtcc gcagaggtac gtttcacctt tcacgtttta cgcttcacgg
    13081 ggccacacgc gactcacgtt caagtaacat ccgtttgcgc tcaacacccc agcgatagcc
    13141 cgagaggcca ccatcgttgc gcacgactcg atgacaggga atggcgaccg ccaacggatt
    13201 cgctccgcaa gcccgcccga ccgctcgcac ggacttgggc gatccgatgc gagtcgcgat
    13261 ctcggtatac gtcgccgtcg aacccgcggg aattcgttgc aacgcctgcc acaccagctg
    13321 ctgaaatgcc gtgccccgaa tatcgagcgg aagtttcaac cctcgccccg gagcttccac
    13381 gaagccgacc acggttgaga ccagccgctc aaatgccgca tccccaccgg tgagattcgc
    13441 caggggaaac cggtcctgaa gatcccgcgt caacgcatcc ggatcatcgc ccagcagaat
    13501 ggcgcagacc ccacgctcac tttgggccac caggatcgtc cccagtgaac agtctcccac
    13561 agcaaaccgg atctcggtct gtgcaccccc ggcccggtag cgtgacgggg tcatacccag
    13621 gagggcattc gatgcttcat agaagcgccc ggaagaattg aatccggcct ggtagagcgc
    13681 ctccgtcacc ggacaatcac gctccaattc aaaccgtacc cgcgtcgcgc ggtgcgcaac
    13741 ggcataccct ctcggcgtca ggcccgtgac ggaggtaaag acccgatgaa aatgggacgg
    13801 gctcatcttc gcgcgttgcg ccaactcgtc gagagacggc aggtgcccgg cctgctcaat
    13861 ataccggcag atggcgacca ccattgccgc ctgccgatca accgacgagg gctgctcggg
    13921 tcggcagcgc ttgcacggac ggaacccggc ctgctcggca tccgcgcagg tcgcatgaaa
    13981 ccggacattc tctcgcagag gctggcgcgc cgcacaggaa ggacggcaat acacgcctgt
    14041 cgtcctgacg gaataataga aggtgccgtc cgcggcagga tcgcgggcca ggatggccgc
    14101 ccagcgcggg tcctgcctga tgttccggtt gatccgccgc gtagtcgaag ccgaggaggt
    14161 cataggcact ccgttcacct tggcatgaat caccacaagg tagcaggcaa gagtccgcgc
    14221 gacactccgg atcctgctgt cgaattcgag aagagcccgg ctgcgcctat ttggtttcga
    14281 cggccgtgcc gtacacctgc gtgcaggctc gcgagaacag aatgaaatac tggaaatagc
    14341 cgtcgaccgt gacttcgacc agcgcatccg ccaagggttt cgtgcgaagc gcatcctcga
    14401 ccgcatttct catctcattg ccacccgtca ccggaatgag gcccagcagg tgataaacac
    14461 aatcctgccc gcgcacctta cccagttcgg tatatccgcc aggggccagc ggcttggtcg
    14521 aaggggcgat gccaccggga tgatgcatac agccggtggt gaacgtcgcc gccaacagca
    14581 ctgcacaagt ccagcccctg acctgccact gcttcgaaag tcgtctcgtc atcacgtgtc
    14641 attcctttcg tgtgcgcggc cgccactggc cacgcaatgt gatacccacg ttgggagcac
    14701 catggttctg atgtctctga ggatcggtac tgagagctac cgggcagagt aaaccgtaag
    14761 agcgaatcga catcttgcac gtctaccgga gcatcctcaa ccttaaaaag cggatctccg
    14821 ggatgtgatt aaatgtgcga cacacagcga acgccccagg tgaaacggcg aacgcaaaga
    14881 tggactgccg aatcagcgga actcacgccg gagaaaaagc agcgggctaa tgaattcagg
    14941 agcactagcg gggattacag gaatgccgaa tttgaatggt gagtcggctg ggattcgaac
    15001 ccaggaccct cgccttaaaa gggccccagg cgataagtaa aatcaatgcc ttacgtgctg
    15061 caacattcca atctgctaca cggttttaca atggcttaca aataggctgt ccgatatagg
    15121 acgtaagccc ggtttgatct tctttggcaa atactgagga tgttcgcgga ccgactgtac
    15181 actgaactcc ctgcttgccg gtgtacgatg tagtgttggg atatagttgc tcctattcat
    15241 tccctgccaa tgccgggtat ctaatctcca tgaaggaata cccaacatat taattcttgg
    15301 ctgccgatca gaaggttttt gaatctcaat tgctgtcgac gcttcactca aatcttcccg
    15361 catcttcttc tgactaacag agactatatg gtttggctgt ctgaaagagg cctgatagga
    15421 cgaaacaatt tcttcggcga acgcggatct ggggaaaact ccatgcctgt ccgtcgtcca
    15481 gcgaaaatga aattcccagg agggccatgc cggcctctta ttataatgca cgtattatga
    15541 cgtaataaaa atgttgacga atgatatagg gccagctatc ctgccagcgc atggcgcgaa
    15601 atggattaat tcgaccttcg cacaagccta tccgataggt gcgtgaggga ataattcgga
    15661 cgcttacaaa ggagaatata aatgctttgg aagcttaaga atcccatccg tttttggatt
    15721 cttttttcgg cactgcttct gtttacttcc actgccgcaa gtgcagagca agacggtcgc
    15781 catcatgatt tgcttaggaa tgcgatcgag gctgcgggga ggggggagtg cccagagact
    15841 ttgatgacac ctctattgat cggtgcgtgt gaagatcaac agccaggtat tggccaacgg
    15901 attagtagct taggaaaaat tagaaaactc agattcatgg gaatccaaaa ttctcagatg
    15961 gggccggcgg aagtctaccg tgtgacccac gagcgcggaa cgatgacatg gatgatcaac
    16021 accgatggta acgggaagat cttagttttc tatactccag gttaacattg aactgaagat
    16081 cgatttatgg actgatcctc agaaagaata tcctatctag gagggtcact tggacattat
    16141 atactttcgt ggggccgagc gcctatttat cgcattctct ggcctaacat gcataattct
    16201 cgggtttgcc ctgtttcgac agacctattc agctgtcgtg agctcaccca ctgaatttgc
    16261 ggccaaaggc ggtggatttg aactgacact acgtaacgtc tggcctgggg ttttctttgc
    16321 ggcatttggg atgatcattt tggttgcgtc agtgcttacc caaattaaaa cacaaggcac
    16381 gagcatggcg gaaatcaaca cattgtacca gagcgggggc cttcctccat cagacacgaa
    16441 aagccgtgca gcagtagcga tcgcggcaat cggtcaagtc ctcgatacag agaataaaca
    16501 acctccggat attgaaatcc aaaggaccaa aaatctctct caacttaaaa cggctcaact
    16561 tgatttagtg gatgttgcct acggcgaggg tagttatacg aagttcttgg agctaagttc
    16621 acgctcaacc gaccccaaag gtttcggcac cttatccgag gaagatcgtc gtttctttga
    16681 ggatctgaag actgctctag atcgttagtt cttgaaattc aaaattgatg tcttgtctgt
    16741 ggtgtcgctc atgttgtcaa tcaccctgca gtaagtcact tggcagcaaa aaaccgcgca
    16801 ttcacatatc ttgccgagcg gggccgtcga tatatggcat tggactggag actcctcgtc
    16861 gcggtatcta cgggttcgtc catgcatgcg acgatgatgg acagtcactt ttgaatgcac
    16921 tcgtgatgtc gatgaggcat ggagccgttc cgaccaccga gctggggagc cactacgcca
    16981 gcgagccgtt ccagtgatcg taagtgttta gggccttctc ttcaaaaaat gcgctcaggc
    17041 aatggctgaa agaacgcacc tatgaaagaa gcgttccaat aggttatcgc gccgagaacc
    17101 gcacattata aagagggcat taatgaagaa gcgggttccg atagttttta tgtttgtatt
    17161 gttccagtgt ttccagcaat tgcctgcatg tgcacaaatc agtggtggga attacgaaga
    17221 ggatttactc agcgaaaaaa caaagctcga cgattatatt aaaaaccttc ggtcgttcat
    17281 atcagtttct ggcaattcaa gtcttaaagc cctggaaggt agagtgcact tccacgtgag
    17341 tgatgagcct gtgcctaatg cctgggtggc acgggacttt caaactggtc gcaacacgat
    17401 atacatgaca gcgcagtaca gattacttac aacctacctc gcggatgctg atgtcattgt
    17461 gtctttagac acgaattttc actcgtgcag acaagactat gtggatacca tttttaaggt
    17521 tctcgctaca aaccgtcaaa gaagcgtctc tcgaggcacg caacgacgca ttccagcgcc
    17581 ggaggtgttc ttaaatacag ctgccaacag ctgtcgtcct ttccagagtc ggtttcccat
    17641 cgatccaaag taccggccag cccgagatca aaccgttaat atggtagtgg ggctgggcta
    17701 tcttcatgaa cttggtcata tcgctatcgg gcatcctccc gttagtctct ctagcattga
    17761 gcaacttccg accagctcgc aaaggctagc ggagttttca cgcctaatgg caagatctcg
    17821 tcttcaggag taccaagctg acgattgggc aattgatcgt tttgtcgatt tatctaataa
    17881 cccgctcgaa tctctgacaa acgtcctcct aacgttttac ctggctttca gcaacttaga
    17941 ctgttcactt gaggcagcgg attctcatcc gaatggttat cagcgtttcg cacggcaaat
    18001 gatacggatg aaagaccggg caacatctgc cgggaaattt accaatactc aggaggttgg
    18061 gcgactaatt gacgacctgc ttacgttcgc aagcaaaacg cagcaaaaac tcgactgccc
    18121 tgagcgagga taagtacgtt gcttactgtg tgtttcaatg cgtccactga cttccgcaaa
    18181 aaagtgcgga agggcgagtg tatacacgac agcttttcaa taaaattacc gctccgataa
    18241 aggtcatttc attgcatgtc ccagagttgc ctgacttgtg caagcgtgta tgccaaattc
    18301 tcccaatatg atagttaccc attggaggag aaccatgtat aaaatacctg caatcttttt
    18361 ttttctattg atatcaagtc cggtctatgc cgaatcaata gacccctcgg acattgctca
    18421 atggctttgt aacgctattt cctctggaga actacagcag caccttaaat catttccggg
    18481 aacgagtggt gaggaggttg tccagcattc atcaccttca ggccccggct ggaagcttaa
    18541 gagttcaaga gtcgaagtga cttatgagta tcaaatagat ggcgatgacc cagaacctta
    18601 tgggttcgaa ttagaaattg aacccgacct tgacgtagca gtgtttagga ctgacgagga
    18661 ggctagatcc tggcttagta gaataggccc tgtgtctaaa gataccttgc ggtctgggtc
    18721 gtcaatgttt acggcaaaag ctggtagtcc atatgtcagc aaatcaggtc gtacaatgga
    18781 aagactcaag gttcaggtga gtatgtcatc ccagtctatt tccgtctcgt ggttttttaa
    18841 gcaagatgct aatcttggac gaaaattttg tcctagtgcc accaatgcgc tcccgtatga
    18901 cgatagattt gagcaatgta tgggagctgc tgtgtcgcgc tgtatatcag agtgcactaa
    18961 caagtatcgc aattctcttg atgaatgcca atctcgcatg tgcagagcgg aaagcccgac
    19021 caataaatcg gcttggacgg ccgtctgtct aagggagcaa cgttagaact aaaggacata
    19081 aatacgaagc atgggccgga cctgttatta agaggtgcga ctgttgcctg tatatgacac
    19141 cgtcattcct agacttactc atttctaaat tgcttttgcg cactcctgga gctctgcaat
    19201 tcttaaagac ctggtgactt gattggtgta attcaccggc atgagacctt tcctgctttg
    19261 ccgagctagc agtgcagtct aaactcttca cggagtgtcc tcatacttct gtcccaggtc
    19321 tcaaactcgc cggtagtttt taaatgctgg atgaatccat tgtcttcata gaacgaaacg
    19381 ataccagcgt caatcattcc acctactcga cacccaggct ctggataatc gcgctgggaa
    19441 gggtcaatac ggaaattctc ctccatcgcc acgtataaca agaatggata tatgccaagc
    19501 aacggattat ggccggaagc tatggccaat cgtaccgcta gcctatccgc ttcagcttcc
    19561 tgaagacgtt ttagatccag gctaagctca gtctttccga catgatccaa aaaatgatgc
    19621 gataattcat gcgctagtat taatgcaaga gcttcgacat tcaagtagga tcttatctta
    19681 tagatccttg gatctgaata gagttctcgt tccttcgtcg cattgaaccc caaaaaagtg
    19741 ggaaatgatt tcaaagcgat cttggagctg cgctcgccat cggcacgact attcttaatt
    19801 agacttgtag cgatatatga aacgtaatcc caggttcggt cctgaccatt ctggagattt
    19861 tgaaacacct gtaaatcact aactaaacta cctgccatta agaaacctac gctaatttca
    19921 atccttctct cgctaccttc tccgcgggca aataccctat cggcttctcg actaggcgtg
    19981 atcctaatcc tgatttgctg tagcgtagcc ttttgatgat cagagagatg tggacgaaaa
    20041 tctttcagga caaggtaaca tgccccttca gaggcggttt gcattgcgtc gatgaatgac
    20101 gcctcatcgt ctgcgaaacc aggcatctct ttcctgggcg gttgtgcatg ctcgcatgcg
    20161 atgcctggcc ggtccatttg caaggcaatg acacctacgc aaattgccgc ccaaagccat
    20221 cgcgatatat gagccgggaa gcacgatagt gtcgctccat gcatgcatcg catcgcagcg
    20281 cctcacgttg caaatgagta gcgcatcagc ccagtcaggt gcttctaaga gcatcagtta
    20341 aaatgttaca aagcgattga tgttgctgag agcacttctc tattctcgtc ccgtccttca
    20401 ttcttatgcg aacacagcct ttctccacct ctggtcgttc acggacttct atgtttccat
    20461 ccgtaatgct tatcccgaga ccgccagtgt gtaattcatg cagtaaacga gtaatcgctt
    20521 gggctagcac gcatatagcg gcagaaccag ccaacgtcac tagcactaca ccggctggac
    20581 caagaccatg caaatcccgc ttctccacca tatttacttg ggctccatta gattcaagga
    20641 atgctatttc gttttggaac aaggcagcat tggacccttc aaactcgaag tcgataacct
    20701 cgtcagtatg atcatcagcc gcatactcag tatgatcatc agccgcatac gtggctcggc
    20761 acgtcaacat actgactaca aataacaatg cgctgatata ttgccacggt cggactcgtt
    20821 tattcatgat agttcaccaa taacccttct atggaattct aggagttgtt gtggcactca
    20881 aatttacttt cccttgtctg gaatagcagc tccccaactt cccataccgt ccgtacacgg
    20941 ctgtcctacg agcgcaggtg tcaatccagg agtacctgga cgaaagtatt gtgtcctgcc
    21001 agctttcggc cccattgaat acctgcacgt agttgataca ccgctatcac tgtcctcctc
    21061 gtcaacctca tcgggctgag tggttcctcg tggatcatgt gattgtttgc cgcatagttc
    21121 cttaatttct tgcttagaga agcctttgtc taccaacttc tcaatctgat ttgccgagca
    21181 ggctgcatat ccagtatgac tatctattat catgatccac atcaaacaga cgaaaagcac
    21241 tcctgaatta tgagcggtcc tcattagcta gtcctcctta gtgcgacgcg ccgttgaagc
    21301 gtgagtaagc tccataagtt gaaaagacgt aataaagggc cagtgcgctt ggttaaatct
    21361 acggatacag ccgggcttga ttgtatccag ttaccctggc agtgagcgtg cataaatcgt
    21421 tgcaaaataa taaattgtta agcttttctt cgtcgttgcg ttttcacttc accccactct
    21481 tattctgctt atcgagagca tctttaatcg ttttccctgc tccaactaag gcgttgatat
    21541 attcggttgc accgggaagg ttggaatcct gggccacgga atcagctcca cagctagacc
    21601 cagtcgcaat ctttcctttg gaaggaagcg cgagacactc gagccatgca ggatcggcaa
    21661 caacttggga cgattcggct acaaatcctg gactcgcctc attggtcacc cgaatatgga
    21721 gtgtcctgca tgctgaatat acaaaggtgt gtgtcttatg aggaaaattg attttatttc
    21781 tttcaaatga gccggcgggt ggagcagcga tatcaatgtc agccaagtag ggcacaggag
    21841 aactgtcttt cagaatgaac cctccctcaa gttcaacttt cgtacatttc attgagttgt
    21901 ccgataattt cgtacaattg ttaggcgggg aggaaagcag acgactaacc aagattccgg
    21961 cgggaggatg ttgacgaact tgagaaatat ctggaatacc cgcggcaaat agaggcaaag
    22021 ccttctccgc tgcagacaca agtgtggtaa tcgcctcaac tctcttgtct gagacactcg
    22081 atcccagctc cttcactagt agtgtgtcgc ccttgtggga cacgtttata tctgttgata
    22141 caccccaatt ttcataccat gaagttccac tgacagaata agtcgtgtca gtaaagacca
    22201 ccggaatcga agtgatgttt aatttattgg tattggggcc tgctgtagcg ccagaccctt
    22261 cgattggata ttcaaacttt atggcagtgt cggaaagctg aaacttggtc agaccttcca
    22321 tatctgaccc cgttacaacc ctgtattgta cgagcggctt tgcgcaccct gccagcatga
    22381 ccaaggtaat tagcagagta ggcgttttca tcatttctcc ttctccttgg ctgtccgttt
    22441 ctgccgtttg gagcttgatt tttttcgtcg tcgcgtgagt cctttcgcac aaaggtcttt
    22501 ttcgtattta gcgcaatggg atttagttgc gacttcacag gccttacaat gtatatgtag
    22561 atcacctgcg atcgtagggt ctgctgtggc atgtgacaat tccaaatgag attggtcaaa
    22621 gcggtacatg aattcacgtg cagccattgg tcctggctta aatattccac agtcacgaat
    22681 atctacggag ccaccttgat tagtaagctg ataggcgtga ctatctgtgt ctacccatgc
    22741 cagcgttcca ccagggcgta catctttggg cgttactaat tttcgattgt agctgtagta
    22801 gatccctggc gtggcattac tatcaaagta attaggcgtg tagaaatcag ataccaaaaa
    22861 tctaccaatc aggtatgcgc agtctttact ctgacagggg tcgcatactt ccaggatgaa
    22921 atctacagtc ttcggagctt ggctagggag ggtcgcactt acccgacggt ccgcgctggg
    22981 gtccaggatc atctccagac actcgtggct tgctgccaat gtccatgtgg caccatactc
    23041 cactaccgca tagggggaac cgtcatcgac cgtatgataa cctgtcagtc caggaacagt
    23101 aatgttgtca atgataaaaa ccggtacata tcccacttcc gggcgttcca gagcggtcac
    23161 cacggcgctg attttccaaa ctggcgcaaa atggtcgatc acttgctgtt gaatcgcagc
    23221 cgcgccagcc gcagcctccg caagcgaaag ttgattgcat cgtggaacaa ttgagatttt
    23281 gatggatggc agcattcgta atctttaaaa ctggcaaaaa ttgtgatctg cccaaactgt
    23341 cgtctagtgg ctgcaatgtt aaggccactg ttgcagtgcg ttatatacgt actatttcag
    23401 tctatagttc gaataattac gcgcctgtgc gtatcttaaa agatactcat gtatctctac
    23461 cgatacgctt aaaggcaggc ccacggcacc ataggaccga tgttagggca caccggtttt
    23521 tctgaaaatt atgaagtcct cccctcagta ttggtctgaa tggatgacgg accctacgaa
    23581 gtcgagatct ccgaatatca ctgaaggaga ccattccata atcaagagcg tcatgcggcc
    23641 gttcatcctg gggaaatcct gttggaggag ttcatgaagc catcagtcct acctatcaac
    23701 gccaacatgc tcgccaaggc gatcgaggtg ccggcgaacc ggattacggc gatcatcaag
    23761 ggacagcgtg gcatcacggg cgatactgcc gtgcgtctat cagcattttt caatactacg
    23821 gctgaatttt ggatgaacct gcagaaagcc tatgagttgc ggcttgcgga acgggctttg
    23881 tcggagaagg tcaagaagca cattgagcaa aatagaaacg cactcgtctc gacgtgatct
    23941 aaatgccgat gcgggcataa tcaacgcaaa ctcatgttga tcttattgga atcaaaaccc
    24001 tagacgttgg ggaggtgctt cataaggcaa aatattgcat ggaaatttgt ccttaggagg
    24061 atcccattta tcagttatat gctcaccgca accggatcgg gtcggagcat catctcctaa
    24121 ctggcagctt aatttgtatt cgattatcat ctccaaagtg tttcttgaaa tgccacgacg
    24181 catactaacc gacacattcc cgttatatct gttccggcaa ctagaggaat aacacaaccc
    24241 cgggatggtt ccgatataaa aacccacatc agtcaaaggt tcgagccaga atgtattctc
    24301 gctagtaatg aatgtgttcc aggtattatc tgaccatgca tagtatccat tttctgccgg
    24361 gcggagtgcc tcgccacggg catcagccag tgctcttgtg tcaattaaat gtaaagtata
    24421 tttacgcctg tgcaggttcc aaactttctc tccctccgga tgataaaaag gacattggat
    24481 tgttagtttc tgcatattga tcctccgtcc gtccacgaca attgcatctg acacgatctg
    24541 agcaagtcct attccggtac catatggcac cataagcgcc aacggcagga gaagaatcat
    24601 cacaaacctt ctgcgagtaa gcatttggtc tcctatgatt attgtccatc taagtggcat
    24661 ctgctggctc tggtggcgat ggtgtaatct tcctgttatc tctaaggtac atctccgttg
    24721 ttagtattgt ttaatacttt ggcttcgaat gcctgttatc aactacggag aaccagcgca
    24781 atcaacccta agctcttgca tcagccttcg aaattgtgga acagaataag tgcttccacg
    24841 ctcaaattcc cttctccttg caggatcgct taaaagttgg tccaactccc cgctagaaaa
    24901 aaggactcgc tgcatgcggc attccgctct cggatgagtc ttcgcggtca taggaatacc
    24961 ttttatgtcc gcttcagatt gcagaaaccg gacaagggta aacattgagg gaataattga
    25021 tatccctgtt cgtcccagca gctttgcggc aaactcatca gccatatcct cctgcttgcg
    25081 tgcctgcgca gaggtaacct ccgtgtaccg tttgtgattt agtgcgagat ggccgatttc
    25141 atgaccgagg acgaaagcaa gagaatcgac cattatgccc tctcgcacgg caagcagttt
    25201 tctatcatcc ggcaatgcgc tggacagcgc atcgactctt gcaaacttgt cgaatgactc
    25261 tagaagaatt tggtcaatgg gtccggaaag aatcgactga ttctgacgct gacgcaggta
    25321 ctcggtgtaa tcattaacga gttctcgcag ttgtggattt ttatgaagat gagcgtgagc
    25381 gtcgacaaga aaccaagtct ggatgcacca catgacagga atgagaatct gtctcgattc
    25441 ataattggca aacgcattga aatcggctgc aggtactagc aacaccagat tcgatttgtt
    25501 tttcaattcg ggatattcga tcaaccccgg gtggagcttg accattgctt catcaattgt
    25561 tcgcttacat gtatcggaca attgtcgcct ggcttcctga cttgcctgtt gcgctgccac
    25621 aggcgcggcc atggcaatca gcgcccccaa aagcgcgaat cgaagtaggc acatattggc
    25681 atgcatgggg taatcctctc ttaggtcgac aggttggtca ccgggcagcg tttatcaatc
    25741 ctgatgatcg ggaataataa attgccttct tgaagttaac tacggggttg ccggagtccg
    25801 ctccctctac aagcttttcg aattgttgta cagagcctgc tcggaaaaac tcgcggatag
    25861 ccccctcata ctcatcttgt gactgaggtg gaccatcaaa tctgtccgcg ccgatgaata
    25921 catcggcgcc gaacgccttt accgcctccc gatacacaat ggcaaaagcc gcacaaacaa
    25981 tatttccagg gtttgcagcc cgcaaatcgc tgtgaaacga cccgtcacac tgcttacgtc
    26041 gaatgctcgc cataacgttc agattgagca ggcgctcatc ggtcatacag atatccttac
    26101 cgtaaagatc gccgccacgt cggcatcggc gatattcaca cacaccatca actgacgttt
    26161 cgcacctccc gtaacatccg tcgtgcttgt cacaggcgct tttgaattgg catttcaata
    26221 gagggattga gtcaggaact aagtaccggt tccatcccgt tccgcatcca ttgggtacac
    26281 tgggcgctga caatgtttgt gccgctgcgg gggcaccgat cagaaacacc gccactatag
    26341 ggactagcat tagctcacca attaacaaac cttgacaact aaccaactta cgtattgaca
    26401 tcatcgcgaa tctccatttc aatgcattga gctcgctcaa taaagctgac taaaaacatc
    26461 tcggaaaggc agtcgtcccg aggtctttca cgctgcaaat gtacagtttt ctagatgcgg
    26521 ggatagtctt ggatccggca tttactcctc gcatcacagg gtgccgttct gtcaaacttc
    26581 atgcgtcgac acagagagaa ctcaacatcc gcttcttcta gatcaggaat gaatcagttc
    26641 actgattcgt cccaatttgg aaaatgagtt ttgagccgtt cgaccgtgtg ccgccacttt
    26701 ggcgtcgtcg gctggcaaac cgtattctga ttcccaaata atccgacctc gttaatggcc
    26761 ttcgattctg ggactgaatc cagccacttt acccggacaa aatactccgc tttctcagca
    26821 tcatctgcat tgggcttata gaagttggca tgcttcaaga cttctaacgc tgaacgctcg
    26881 ccagatgggg tgctcaccgt gaactctgta atgggccgaa cggcctcaat aaccctggca
    26941 actcccacgt atcctgtctt tggaatcttc acccagaccc tatcgccagg tgccaacatc
    27001 ttcaacgtct ggctgtacca actccctcct ccggcgctaa tgtagccata ctgacgcgca
    27061 tcctcccagt tccgagctgt catatcccca aatgagacgt agtactcgcc gttccaaggc
    27121 tccttttctc ccctgctctt cgttgcaact acatttgctt gggtctcttg cggatcaatg
    27181 agccaagcgc gactgagaag ttgatcatgt ccttgctgaa atacctgaaa gaagacgacg
    27241 ttgatcgcga tatcgtgcga attgaggtag ccgacaatgc gctccgtagc cggatcaagc
    27301 tctgctgcaa caatgatgac ttggtgcgat tggttgaggg cttcctcatc caactcgact
    27361 ccgaaacgtg catgaaaagc ctcgtcgagg tttccgccac ccgaaaagcg ctgatagatc
    27421 tgtgcgatct tttcggaagt aagctccgca atccaggaag cgtagtccaa ggcctgagca
    27481 atagtttcac ggggcgtgcg gtcgcgcttc agctcaatca ataccaatga gccatccggg
    27541 gcgatcgcca gaaggtcaat acagcctcca tgactcgtcc tctcttgccg tccaattatc
    27601 atccactcgt tcgagaggat acgcggatcg ctaacgatca tgtcttcgag ctgttgttcg
    27661 cgtgcgagac ggctcgttgt tagaagtgtg ggcttgtccc caactttcca gatagcgtgt
    27721 tggataggta tagttcacct acatttccgc aaacaatgct tcaacccaat caagatggcg
    27781 atgcagtcgt agatagtatt ttgcagccga actaaatggg gcgcgcgctt caattctttc
    27841 cttcaatcga gtcctgatga tcgaccgtgc ctccgcgcgc tccgccgact cttcatacat
    27901 ctgtacgtca tgggcaatct cattgcaaag ttgctcacgc tttgagttga agtctggatg
    27961 gtcaaggttc agcaatatct tgctttcttc gactcgcctt tgcgcaatcg catcggcagc
    28021 gtgcgcaggg ttcagcaccg gacggccgtc ggcctgaaac gcaatcaggt cgcagtctgc
    28081 tttgtcgcaa ggatcgagaa tgactggctt ctcgtacttc aacttgtttc tgcccttggc
    28141 acgcagccca ggtccaagca acgggaacac atcgcctttg ccacctgcgc acccatgcag
    28201 cttgttgtgt tcgggactgt tagcgaacgc gcacgccact cggtaatttg cagtttcgaa
    28261 cgcgagccac cagtaatcgc atacagggcg aaagtgatcg attgcgagcg gtgcgccgac
    28321 aagctccacc tccgtgtacc agcacttctt gccaagccgg tcggttaacc agttcttgac
    28381 ctgtgaccac ttactccgcc cgttcgtgcg gatgtaccgc tgacgctctg cgggtgacat
    28441 cgcgaccagg ctcaaatggc tctgaaagag tcgcacccgg tgcctggcac cgtcaggttc
    28501 ggaaaatagc tgggcagtca attcatgaac gggtatatat cgcatcgcga acctcagttc
    28561 ggcactacgc gacgccgcag gaattcgacg taggcagggt ctcgttcgct gacccctggc
    28621 gacaggattt cgagctgcga gttcagcgtc ttcaatcgca atttttcggt actggtgagg
    28681 aactccttga tactgatcgc gagtcgctca tccaggagtt tctgcgtctc cttgtccagg
    28741 ctagaaggca gaccgaaata ctcactgctg caaagaagat tcgcgatacc ctggccgcgc
    28801 ggatggcgcc gaggtcgcat gaatgccggc cctgcgtccg acggatggcg agccaatagc
    28861 acctggtcgc tcgtcatacc ggagatcatg atcggcgtat gggttgccat cagaacagtc
    28921 gagcggcttc gtcgctcagt atccagcgca tccgtcagca tcgatgggta atcccaagac
    28981 cactgcgggt tcaggtgcgt gtcgggctcg tcaagcagga cgagattgtc gggactgttg
    29041 gtcagcttga gcgcaccgac aaccgcgagt agctgcttct caccctcact cagttgatcg
    29101 aactcgaatg gctcgtcgtt actacgcccg attaaccgaa aggacacaga accgaagatg
    29161 cccctggcgc gcagatgctc cagcgcgagg taaatgttgt ccccaccgcg tgccaggcgc
    29221 gtggccaatg ctgtgagccc cttatccgcc cgtttgtcta catcgaagcc atacgtccgt
    29281 tgttcggagt atgccatatc atcctcagtc cctggcgcag gacgccgttt tttccgacga
    29341 ctctcttccg acgaagcagt ctcgtggatg gcgccaatga cccggcgaac cgcaccgacc
    29401 gtattccaga gcttgggctc gtgctgtttc gaatcgaagc tcgaaggcga gcgaagtacc
    29461 aagacgacgt cgatgagtcg cttcagcccc aagcgatcga gcaagtcaag atgaccgtgc
    29521 gcatacaaag ccagcaggat gacttgggcc tgcgcgttgg tggtctgaac aaaaagaggg
    29581 cggtttcggt cggactctgg tttacgcgtc agacgctcga aatgacgacg atagctaccg
    29641 atgagttggt tgactcgttg gcactcgcct gagtaataga ctagcgtcac ctcggggtag
    29701 tagacctgtg ccgcaccttc acgaaggcga tctgcaaaga atctgagcga cttctcttcg
    29761 ccgtccacca tcacagtcat ctttcgattg tcactctcca gggtgatccg acgattctgc
    29821 gcttcaaact caaacatgaa gtcgaatggc ggtgccttct tgaagtaaac gccaaggaga
    29881 atgtgaagaa tggcctcaat gatgtttgac ttaccgctgc cattgcttcc aatcaccgca
    29941 ttcagcagct gaggtcgcgg aaagtctatt tcgcagttgc gcaggttttt gtagtcctta
    30001 atccacaacc gctccagctt cataccgata cgcgtccttg cgttaactca gcaacgacgg
    30061 cgtcgacgag ttcagatcct gtggtgctgg cagtggcgag ctgggtttct aacaaatcca
    30121 ccaaggccgt cagttcgtcc actttcgcca cgatccggca ttgttcggcg aggggtggga
    30181 gcgcaatggg aatactgttc atcttcgcct gattcatctt cggttgggcg gtgccggtga
    30241 tgtatggctc aaggacgatc gcgttgatgt gtagttccat gaaccgtagg aagtcctcgg
    30301 agattccatc gagaacgtgg gcgtgattgt tcacccagta tttgcctcgg gcgataaagg
    30361 cgatgggcgt cgatcggttg atgaggttcg ccccgtcttc gccgacgaga aggagcggct
    30421 tatcgaagag gtagttatcg atcttgtcga tgaccccaga tgcgccatag tagtcgtacg
    30481 tcttcgcttt agttgcgcgt tcttccttac taacggggat acgctcgccg tctcgattga
    30541 ccatgatgtc gccgaagcgc acccacttcc aagtcgaggg tagctcgaag tcgccttgat
    30601 ccgcagccat tggagtgacc aatgttgagc cgcgaagtct tccttctctc gacaaagctg
    30661 ctttgtgagc cgcaatacgg tcgagcagcg caagcgccgg ttcgtcgttg cggtcttggg
    30721 ggacaagttt gccttggacc gcgagggtga ggatggagct gcggagctcg gctgggggaa
    30781 tgctgtagga cttgtgaaag agcaggccga ggttggccgg ggtgggtgcg tcggcaaagc
    30841 gacccagcga cgcgcgggcg agctcagcgt gtcgcgtctc ccgttcctgc tgctgcgcct
    30901 ccagccgatc acacaacgcc atcaactcat ccaccttcgc cacgatccgt ttttgctcgg
    30961 cgaggggtgg gagaggaaat gggaacccaa tgattttgac tttagagata ttcggctgcg
    31021 ctcctccttc acccgcagca tgaaaacgta acctttgcga aatcagaaaa gtgaataggt
    31081 agtggttcga aactccttca aagggtgtgc atccgcagac cgcttggttg gtggctgcgg
    31141 gttcggcgag aatggcaacc ttccctatcg ttgccccata catcgcgagc agcacgtcgc
    31201 cgggttggtt cagccggaac gaacaagtct tcaaggcgag ttccgtgact gtctcctctg
    31261 agcccttaag tgcttggttg tcattaagtt cgccggattt cagccaagtt atcccaccac
    31321 catacagctc atgatttccg cgcgtcggag ttgagccgga tccccagtct ccgatttcac
    31381 ccagacgcgt ccagacccat gaataaggaa taagaaaagg cgcttcgtct tcaccaagtg
    31441 gttcggtgtt ctgttgcctg attgcccgct cggagattaa ccgatccttt attcccttga
    31501 tgcgttcgag gagagtgttt gcgggttcat cggctggatc ttgatcaata agctttcctt
    31561 gcacagccaa ttgcagcacc aactcccgca ttttcgctac cgcatcgggt gcgtcggtaa
    31621 agaggtcaaa cttctcgaag aaggtctcca gagtcattct gcagttccag cgctggcggt
    31681 gagcgcgttc tggagttcag ccttcaatgc ggcgcgggta gcggcgatct gacccaggag
    31741 cttttcgtat tcaggcagca ggtgctccac gtcgcctgga ccggtgtcag gattgtgcgg
    31801 gtttttgaga tcaaggttaa agttgccggc cttgatctgg tcgatagaaa cacgccaggc
    31861 ttgctcgttc gccacgcggg cggcgaagcc attcgattcc tcgccccacc aagtacgttc
    31921 cggctcgaac tcttcgatgc ggatgggttt gcccttgttg taactcttgg ctccaggcgg
    31981 atacggatgc tcgtagtacc agatctcagt ggtgggctga cccttggtga agaagagaag
    32041 attggtacga atgccggtgt agggattgaa tacgccgttt ggcaatcgga cgatcgtgtg
    32101 cagtttgcac tcgctcatca gcgcttcctt gatgcgcgtc ttgacgcctt caccaaacaa
    32161 cgtgccgtcg ggcaagacga ggccggcacg accacctggc ttaagaagct tcatcagaag
    32221 tacgaggaag aggtccgccg tctcacgggt gcgaaactct gatgggaagt tggactcgat
    32281 gccgtcttct tccatgccgc cgaacggcgg gttcgtaacg atgacgtcta cccgctcctt
    32341 gggcgaccag tcacgcagcg gacgggccag tgtgttgtca tgccggacgt tggagggaac
    32401 atcgatgccg tgcagcagca agtttgtcat acacaaaacg tgcggcaggt gtttcttttc
    32461 gatgccggaa aaacactcct gaatgaacgc ttcatcggct tcggtcctag cctgcttacg
    32521 caagtgctca atggcacata ccaagaagcc tcccgtgcca caagccgggt cgaggatggt
    32581 ttcgtgcttc ttcagactgg ggtttacctg ctcgacgata aattgcgtca cggcccgtgg
    32641 ggtatagaac tcccctgcgt taccggcgga ctggaggtct ttgaggattt tctcgtagat
    32701 gtcgccgaac atatgacggt catcggaggc attgaaatcg atgccgttga tcttgttaat
    32761 gacctgccgc atgagggtgc cattcttcat gtaattgttg gcatcctcaa atgtcatgcg
    32821 gatcagaccg gcaatcttgt ccgcaccacc agtcaacgac ttgagctttg gcaggagggt
    32881 gttgttcacg aaatcgagta gcccttcacc cgtaatgccc tcttcatcct tggcccagtt
    32941 tgaccagcgc aggtgcttcg gtaatggcga tttgtagttg tcacggagga gttctaattc
    33001 cttttcgcgg tcatcgaaga tcttgaggaa gaacatccag ccgagctgtt cgagacgttg
    33061 cgcatcgcca taggtgccgg cgtctttccg catgatgtcc tggatggttt tgacgatgtt
    33121 ggaaacgttc gacatatgtt acgcggcttt ctggtataag gcggtttcga gttctcggat
    33181 ggcggccaga tagtcactcc tcccaccaaa cagcttgacg atttcgaccg gtgttccgaa
    33241 gtgcgtcagg ggatcgacct tgaggatttc gagcgactcc acgctcttaa tacccccatc
    33301 ggcatacttc tcaagcaagg catcgagcac cgcgcgggtc ttctcaccat acttggcgaa
    33361 gacgtttccc ttccgtacat tttcggcccg ctcgcggcgt gtaagcggaa attgatcaaa
    33421 ggagacatgg caaatgagat caaacgcgtc aaagcctaga ccaacctgtt cagcaagttc
    33481 ctccaaaaat acaccacgac ttgaaagctc ttcgacgatg gcctgtttgc gatctgcatt
    33541 cttccaggca ttcaaaaagg cgtcgagcga cgcgtaggca ttccgtaccg tcttgcgcgt
    33601 gtaatccttg agcgattcag taataagctt cccctcggcg tccaagtact gaacccgttc
    33661 ggttgcgacg gttacctcca catcgttcac gtagtacttc gctggacgtt catgaataac
    33721 gggcggctct tcgccattac gtgaaccaag ttcagcatag ccctcaggat tttcttcatc
    33781 actctcctcc accccatcag gaggaactgg tgagtccttt tggccaggtt cataaatctg
    33841 aacaggctcg ccatcgaatt tcggatcggc aaacaacgcc gtggcccttt tgaaatccat
    33901 gatggtgaag aagagcttat tgtagtcctc attgatgcgc gtaccacgcc cgatgatttg
    33961 cttgaactcg gtcatggagt tgatggtctt gtcgagcaca atgagatggc acgtttgagc
    34021 atcaacaccg gttgacatca actgcgacgt cgtggcgatg actggaaatg ttgactcggg
    34081 gtcaatgaaa ttatcgagct gagccttccc ttcctcatta tcaccggtga ttctcatcac
    34141 atacttactg ttagcagcag caagatctgg gttagcgttg accagcgcct gacgcatccg
    34201 ctcggcatgg ggaatgtttt cgcagaagac gatggttttg gcgaaccgat ctgtagcctt
    34261 taagaattca gtgatcttcg acgcgacaag ctccgttctc ttttcgagga tgaggttgcg
    34321 gtcataatcc gtcacattgt actcgcgatc ctcgatcacc tgaccatact tgtccgtctt
    34381 gcctttttct ggtcgccacc cttccaggtc tttatcaatt ccaatacgga ccaccttgta
    34441 tggggcaaga tagccatcgg aaatgccctg tttgagtgag tatgtgtaga tcggatcgcc
    34501 gaagtattcg atgttggaga cgtccttcgt ctccttgggg gtggccgtca tgccaatctg
    34561 agtggccgac gagaaatact ctagtacctt gcgccagttg gcatcttcgg cggcgctgcc
    34621 acggtgacac tcatctacga taataagatc aaagaaatca ggagagaact gcttatagat
    34681 attctgttct tcttccgttc ccgtcactgc ctgatagagg gacaggtaaa tctcgaaggc
    34741 tttatcgact gtacgattgg tgattttggt cattgcttga ccgaacggct tgaagtcgtt
    34801 ggtcttggtc tgatcggcga ggatgttacg atctacgagg aagagaatcc gcttcttggc
    34861 gccggacttc cagagacgcc agataatctg gaaagccgtg taggtcttcc ctgttccggt
    34921 agccatgacc aacaagatac ggctctcgcc acgagcgatc gcctcgacgg ttcgattaat
    34981 ggctatcaac tgatagtagc ggggtgtctt gccagatccg tcgtcgtagt actcctgcgc
    35041 cgtcactctc tcctgatggg ctgggattcc cttgacagct tgatatcgcg accaaagatc
    35101 gccgggtgag gggaattggc cgagcgggat ttcccgttca acggcgccaa ctattcccgt
    35161 tctatcatgc tcaaggaaag cgtccccatt tgagctgaac gcaaagggca catcaagaat
    35221 ctctgcatac tcaagggcct gctgcatccc accaccgact ggatgattgt tgtcttttgc
    35281 ttcgagaaca gcgatcggga tgccaggctt ataatagaga aggtagtcgg ctttcttggc
    35341 ttcaccgcgt ttgacagttt tcccgcgtac gatgacacgt cccttggtga agtagaactc
    35401 ctcccggact tgagacatga catcccactt gccctctgga ccgatgagcg atggggtaat
    35461 gaattttgtg cggatgtccg cttcggtcag ctctttctta ttcatgactc tgccagatct
    35521 gctgccgcag aacgccgttt tcctgcaaat gtgcggaaag gagataccgt gtttttccaa
    35581 acaaatcaaa tagtccgaag ggggatggaa cagagatcgt tccaagaagg ctttatttcg
    35641 ctaaccttga gcaaagctaa ccctgaaaat ttcccacaag aagatacata gagccgttcg
    35701 tacctacttg gacgtttttc ggccctagtg caattttttt gcaatattat gccctctttt
    35761 tgattgctgc gacacgacct ctcgactcac cgcggtccca ttcgacctat catcgcctaa
    35821 tcgcttatcc ggcacgagcg tgtaaatcac ctctcccacg gcggcgatcg atcatcgctc
    35881 aataacagtt ccgtcatctg acaaccttct tgcatacccc gcctccgact tcacctcgtg
    35941 tgctcaggaa cgctctgcat gggatgccgt agtgtccctt agtgtcccct ggtgtcctct
    36001 agtgtccccg aacgggcctc tttcaccaca ggacgccgat gcgtatggtt cggccataca
    36061 tcgagcgccg aaacgtaaag gagacgtgat ggaacaagca tggttaaccg tcgatgagtt
    36121 ggccgccgtc ctcaaagtca gcccgaagtc aatccgccgg gcctaccgca aaggcgaaat
    36181 tcccgtggat cgattttgtc gatttgtgcg cttcgacctg gagcgcgtga aagaggccct
    36241 gcaggcgaag gggcatggtc cgtcctctgt ggtgtccccg aggaaggggg gacagcgcag
    36301 cgcgaccggc ggcggcagcc ggcggcgcgc gcagcggacc cgcccccgac ttggtaagac
    36361 gggggcgtct atcgcacgga ccccaaggag gaagaaatga cggcttcagg aggattcact
    36421 caaacgattg attggctcgc cttcacgctc ccgaaagctg aggtggacga ggtcatcaag
    36481 accattggcg gggattggtt ccagagtgag acagggtttc gtgggtatcc cgtcgcgaga
    36541 ctcatgacgc agggcaagac cggtgtcgcc aaattgggga cgggtgcgcc tcgcaatccg
    36601 aaggaagtgc atgtggatct gtccgccggg attgtctccc agtgggatga gacgaaactc
    36661 aaaaccatcc tggcctggat cttcgctcag aagggtcacg tgacacgcac cgacgtggcc
    36721 ctggatgacc gggaggcgac tgtcgcagtc gagacggtcc gacaagccgt ggaagccgga
    36781 caggtggtga gtcggtccaa gcaattcaag gtgatccaag cctcgaatca tcgtgaggga
    36841 atccgaaccg gagaaacact ctacttcggc agtcgggaaa gccaatccat gttgcgtgtc
    36901 tataacaagc ggctggaact ccaagccaaa ggccgggagg acgcggtttc gtacggcgtc
    36961 cggtgggaaa tggaactcaa acaggatcgg gctcaagcct gcgccaaagc actgctcaca
    37021 ctcgattcag aagattggcg ggcatttcta gtcggcgtgc tccgctccta tgtggatttc
    37081 cgagagacca cgcgagaagc ggagtcctat gaaaagtatc gggctcctct gttgggttgg
    37141 tgggaagccc tgaccgaagg cttcaggcga tgtcggctcg tggtcgaacg cctccagcag
    37201 cgactggatg acgtggccgc ctggctcgcg caatccgtcg cgtcaatgct cgccgtggtc
    37261 gtggcctgtc gaggggatca atttctcacg gagctgatct atgcggggac gaagaaatgg
    37321 aagcagaagc actatgccct gctgaaggaa cggaggagcg gaacacccta tgtccttaca
    37381 atttcgtgac ggagggtggc aacgcatgtg tccgggatgt ggcgcgagag gctgtcggtg
    37441 caagtgggta caaggagccc tgtatttgag ctgtcgggaa tgcgattcgg aattctgctt
    37501 catgccaaaa gcccggtccg aaggccgggg cgtgtgggtc aggcgcttgg ctgacccgaa
    37561 tttcactgat cactggagga gggacgacga tgcaagtcaa agcggaggga gcggtgcagg
    37621 ggtatgtgga acggagaagt cgagagggga aagtctaccg atcggtggat ctctatgtga
    37681 agggccgaga tccgggggtg cttcgattgg ggattccgga ggatcaggtg cccttgatcg
    37741 acgcctgcaa gcaagccgag ggcaagcaag cgaaagtctc gatcgaagtc cggaagttcg
    37801 agcagaccgg acgggtcttc ttcgatctgt acggcttgga agtcttgaaa taacggcaga
    37861 gggaggaagg tcagtggatc tcacgataat tctcgtcgcg gtcttgttac tggccttcct
    37921 caccggcctc ggagtcggac ggttatgaaa cagtgtttac tcaccagtta cctcgggctc
    37981 atttggacac ttctgatttc cttctgtctc gcgcccggcg agtcgctggc tctagaaacc
    38041 acacagtact cacgcgtggt cgctcaagcg gaacgtatcg cataccttgc ggcccaacgg
    38101 tcggccttag cgagccaagt ggccacagcg gcccttactc cttccgccag ttccttcgct
    38161 atccgattag tcgcaggtcc ggtcggttgg gccgcgctcg gtgtcggcgc gggattagtc
    38221 ctggcccaga tgtactattc gcaaccggac ctcgcgtcga tcaagacagc atcgtcaacc
    38281 cctggcggct ggcaagtgga gagtaccaat gccggcaccc agacttttcc gggactcggc
    38341 accaacagcc ccgccaatgc gaattacccc aatgcgtcca ttcaattcag cgcgaccaat
    38401 gtccccctgt gcgcggtcga taccgagtat cagcatgatt gggtcgtcgg accgtttcag
    38461 agtgtcagca cggcggtgtt ttttacaggg aactttttcg tgaatggtcc cgctgttggg
    38521 ggccagagcc tctatgtctg ccaccgcaaa ggcatccctg ggtcaccggc tcccttacaa
    38581 gacgccctca tcccgccgac tccccagcag gcggccaact atgtgacggg actgccggcg
    38641 agtgatccca aatcgatcga agcccatacg aatcccgtcg ggacgaccgg caccacgcaa
    38701 ccggcggaca ataccgtctc gcagcctgtg tccccaagtg agatgccgac gaccgtcaaa
    38761 ccgaagcctg tgcctgcggg agacatcatc gtcgtcgata gtgtcgcgcc gccagcgagc
    38821 accccgcagc aaaacacgca acagcaaacc accacgacga ccacaacgac cactcaaaat
    38881 cccgatggct ccacgacgaa gcaggaagag acggaagcca ccacgtcatg caccgcaggg
    38941 gcacatgaga atcggacgtt tgggacggtc ttgcaggcgc atcagaccat ttgggcgagc
    39001 agcggattgc tcagcacgct gaatctcctc aaatccctga cctggccctc gacgttgccg
    39061 gtgattgcgc tcccctcggc cctcttcggc aaccagcaag tggacttcaa ccagtgggtc
    39121 tggttcttca cggtgctccg cacgatcgtg atcgccattg cctccttcgc cgcctatcgc
    39181 atcatcttcg tgggaggagg acagacgaca tgacggcgat tctgaccctc atctattgct
    39241 ggctgcagga gttcttcttt tcgcttacgg attggggcct ggggatctgg gattccctgc
    39301 tttccgtggc ggacagcacc ttgagcacca tgggcacggc aggcctcacg ctgccggtca
    39361 ttcctgatca atatgcgtgg gtgctgggcg cgacgggcat gagtcaggcg ctggccatcg
    39421 tggcgagcgc catgggaacc cgcttcattc tccaaacgat tccattcgtc cggtggggct
    39481 catgaacatg acacctgtga gtgtggccta tggatggacg gctcttttcg ccggcactct
    39541 ctggctactc tggaggcgta ggtaatgatc gagttgtatg aaggtgtgcc gggctccggg
    39601 aagtcctatc acgcgatctg cgagaagttc ttaccctggg tccggcaagg acgacgactc
    39661 tatatttcgg tggacgggat ctacctggat cggctggcgt tgttcacggg aatcgaactt
    39721 tccgtcctcg aacaacagat caccatctgg aaagactcgg tcgaagtgct tcaggccttt
    39781 ccacatgtcg agccgggctc ggcggtgatt atcgacgagg cgcagaccgt attccggtcc
    39841 atgcagaagg ttgagccggg tttgctgcgc tggctcgaaa cccatcggca ctacggcgtg
    39901 gatattctgc tcatgagtca ggattttcgg caaatgtcgc aaggcgtcac gagattgatc
    39961 gaagcgacgg tgaagtttcg gaagctggcc tttgtgggcc tgtcgaaaaa gtatcagggg
    40021 aaagtgcgcg ggaatccaga agatcacgag acgattcgcg ccttcgtcgg cacctactca
    40081 cctgcgatct acgcctacta ttcgagttat gcctcggcag cgatccaaga agagaagcgg
    40141 agccatacga ttttcaagtc ggcgcgcatc gcgatcggca ttgcagcggg gctatttgcc
    40201 attggcctct tgttctggcg gccctggtcg tcgttgaaca gcacgatggc cactccggca
    40261 actggagtga gtgggacggc cactctgcct ccctcggcct cggcgtcggg cagcctcgtc
    40321 aatcctttgg gagtctctgt ctcagtggcc gcaatcccgc agccacccaa acgactggtg
    40381 cgaatcctcg gcggcgctgg gtcatcggcg aaccggcaag gctggcgcta cttgctggac
    40441 agtggggaga ttctgacggc ggcgcagatc accggacgct atggaatagc ggtctcagaa
    40501 gtctacgagg atggtgcaat gcgagtaatt ggcgaaggag tgttctatgg acctgccggc
    40561 gattgaggcg atgacctatt tcacagcgat cttctggctc ggcgggtttg ccgtgggact
    40621 catcatcaaa ctgatcctgc ctcagagcca ctgaggcgct cgaccgccgg cgagcattcc
    40681 ggcggcgcgg gtggacgagt ccaccagata gcacaaggag gtgctctatg aagggtgtgg
    40741 gatgggtgaa gggactcacc gggttcctga tggcgttggt gttcgccgta ggcgtgccga
    40801 cgctgtcgca ggcgcagttg tttccggtgt cggcggatgt ggccacggtg cgggccgatc
    40861 tgctcttgtg ggcggcggcc ctgattgggg tggcgttggc catctatgcg tttaaacgcg
    40921 tgaaggcgat tgtgggcggg taaggcccat cgaggcaggc tggcagcagc ctgcctcctt
    40981 tcccacccgg tcaacacaat gagaaggtca aggacgaagg aagacaacgg atgacggcgc
    41041 aacaaattca aactatcggc aatgcgttag cgaccgacct cgtcgcctgg gggaccgcgg
    41101 cgatcgggct cgccctggta gcggctggtg ccgcctgggt gttgcggctc ctgcgctagg
    41161 cgccgggcca accttgatcg gcgaagctgt agtagcgctc ttcggcgagg atgagatgat
    41221 ccagcaacgg aatgccgaac agatctccag cgtcgcgcag ccgtttggtc agaacgcgat
    41281 cttctgggct cggggtggta tcgccggagg ggtgattatg gccgcgtgcg acgtgaagtc
    41341 gctttgatga ttgttcgata cgaaagtaag agaacctgcg cttccgtgcg cagtagccta
    41401 tcggcacagg tgtggcgggt aaccgccggg tctggagctg tcgagacaac gtaccctgac
    41461 ccacaagggt ccggcatgcg gccgtgaggc cgccatcggg gctgccagca tcaggcggct
    41521 cgggggcaag gctgggtggt atgtctaatg aaagtgaact gctgtcaggc accgtcaaaa
    41581 tcagtcgagc caaagatgct gagtggctca ggccaacagg tatcgcggtc ggctgctccg
    41641 cagcgcactc ggagcacacg gacattcaag accgccggtg tatcggcaga ggctacccca
    41701 ctcgtgtcat caatgcggaa cacggtaaac ccgatgtccc gctcccgtga gggagcaggc
    41761 gcgctcgcaa gggccgctgt ggggatagcg ggcaaaggaa gctggagaaa gcgaacgccg
    41821 ccctgtaatg gagcggatag gcgttgcaac atcacgctgc cgcgaaagcg ggcccacttc
    41881 cagtcggtcg ttcgtgacga gaaagattgt agaaccacct tgggaggaaa tcgcaaatga
    41941 acgtagcgca cgctgcgtgt gcatcctcca acggagcgcc gcagtggaac cagatcgact
    42001 gggccgactg tgaacgcgac gtgcggcggc tgcaagcacg tatcgtcaag gcaacacggg
    42061 agggccgcca cggccgagcg aaagctctgc aatggctgct cacccactcc ttctccggcc
    42121 gtgccttggc cgtgaagcga gtaacgggga atgcaggcaa ggagacctct ggggtggatc
    42181 acgccgtctg gcgctctcca gaggccaagg cacatgctat cggatcgctt cgacggcgcg
    42241 gctaccagcc gcaaccgctt cggcgcgtgt acatcccgaa ggccaacggg aaactcaggc
    42301 cgctcggtat cccgacgatg cacgatcggg cgatgcaggc cctgtatctg ctctcgctcg
    42361 atccaatcgc ggagaccacg gcggatcaca actcgtatgg cttccggccg atgcgttcga
    42421 cggcggatgc gatcgagcag tgtttcaagc tgctctctcg caaaagcgct ccccagtggg
    42481 tgttggaagg cgacatcgtc ggctgtttcg atcacatcag ccacgactgg atgctcaacc
    42541 gtatcccgat ggataaagcg atcctgcgag gctggctcaa ggccggctac gttgagaagc
    42601 gaacgctgtt cgccacggag gagggcacac cccaaggtgg agtcatctca ccggcgcttg
    42661 cgaacatgac gttggatgga cttgaacgtg ttctgcaacg ggccttcccg cggaaggact
    42721 tggggcgagg gcgtggcaag tacaatccca aggttcatct cgtgcgatat gcggatgact
    42781 tcatcatcac gggaacttcc gaggaggtcc tcaggaatga agtccgacca ctggttgagc
    42841 agttcttggc tgtacggggg cttgcgctct cggcggaaaa gactcgcata acgcatatcg
    42901 acgagggcgt cgacttcctc ggccagcatc tgcgcaaata ccatggcaag ttgctggtca
    42961 cgccttcacg gcagaacgtc catgcgttcc tgcagaaggt gcgagcgatt atccgagcaa
    43021 actgcgcggc ggcacaggcc gatctgatcc atgcgctcaa ccgggtgatc cttggctggg
    43081 tgatgtatca ccggcatatc gtggccacgc gtacgttctg caaggtcgat catgtgctct
    43141 ggcattgtct ctggcgatgg gcgcgacgca ggcatcagaa caagaacgcc aattgggtgt
    43201 tgcaccgata ctggcatccg gttgacggac gaagctggcg ctttgcggcc gatacgagtg
    43261 tacgcacgct ggaagggaac ccggactggt tgaagttggt ctgtgccaac aaaaccatca
    43321 tccggcgtca cctcaaaatc cgggcggagg ctaatcccta cgatcggacc tggcgagcgt
    43381 actttgagga tcgtcgctcc gcgacaggtc actcgcgtcg gcggctccat ccaccacccc
    43441 cgttgtgact gtccggctcg gcggcagcgc cgggctttgt aacggcttga gccggatgag
    43501 cggaaaccat caagtccggt tcttagggga ggatgcggct gcaaggccgc atccttaccc
    43561 gacaaatcca ggcgccggca ttcatcagga tcaagggctt gaagacctcg cgaggatgca
    43621 caatggccaa agtcagcgac ccggtggaga cgacattgat gccgatgagc ccatgtttgg
    43681 catcgaggcc gcagatcaga aattgctccc ggtccaatcc ggcgaacagg ggcctgagga
    43741 ttgcggcagc gccttccgac gtatgcaccg attccgcagc tggaatggca cggccctcac
    43801 gcacgagcgt gacgcgatac cgagggacac cgtatttccg aaccgggttc agtggccgtc
    43861 catcgtttga ggaaccgaga gagctatcaa cggacatgtc cgcctcctta ttcatttcta
    43921 tgtagcccca cccttcacaa accaagggtg gggcgtagaa gaagaggcgg cacggggctc
    43981 tcgcagtggg caggcctgga cggatgatcg gaggtcgaat cgggagagtt gggagggcct
    44041 gaaccgctgc tcccaggcac cggggcgcac ccacttaaag attcggtttt cgtgttaagc
    44101 cggcgacaca taagtgattc agtgagggta tggtgaggac gagtcaagaa cagacgcaag
    44161 gcaacgcgac actagaaatc atgaccctgg ccgaagcggc gatctatctg cgcgtgtcga
    44221 aatccacgct gtatcagcgg aaggatattc cgaggcatcg catgccagga tcccgagaag
    44281 tcagatttct caaagacgag ctgatcgcct ggttgaaaag cggtgataga gaagcgcttt
    44341 cggtggcgtc gcgcgagctg gatttcccac tggacagcgc tcatgccact gtttaccatc
    44401 ggaacccaaa atatcgggga tgaccatggc gtataccatt acaaagcgac gcacgaccaa
    44461 aggaatcgcc tttgacctat attttcggtg gaaggggcaa cggtatcgcc ccttgctggg
    44521 ctatgacctc tcacccgatc aagcacagca gtgtgccatc gaactcatgc ataagatcca
    44581 aacgcaggag gtcgagaagc cgcgttcgct gatgaccctc cgcgatgtcc tcccgctgta
    44641 ttggcaagcc ttcgaagtca aaaagcgcat tgattccacg cgaccaaaag ggattctgga
    44701 gaatcacatc ctgtcctgtc atgcatgcgt cagaaacgac ttatcctgca gtcatcgttt
    44761 tggacaccag ccgctcgctt tgctcaagcc ggaagatggg ctgagatatg tgaaggcccg
    44821 gctggacgaa ggagccacgg ccggcaccgt ccggcgtgaa tggcaagtcc tcaatcggat
    44881 tctcaatgtg gccgtgcgct acgagaaatt ggacagaaat ccgctgaagc atgtcgagct
    44941 gccggacgcg gagaaacgaa ccagaatcgc cgagccagag gaactgtctc tcatcagcaa
    45001 gatgcgggaa aacgacccta ccaagcttga atgccggcaa gagctctggc gtattatcca
    45061 ggtcgccgtc tcggtcggcc tgcgagaagg caaaattctg gagattgaac gttcgtggat
    45121 gaagaagcga gaggatggat actggttgtg tctcccgcca gccgcaagcc gcatcaaagg
    45181 caccccgcaa gaagtgccat tgaaccgcac ggccttactg gccttgggcg ccgagatccc
    45241 gtctctcgcc gatggtcggg tattccgaca ttgggtgcat cagcgggcat ttaaggaata
    45301 ttggagtgaa acttgtcggc gggtcggaat tcatgacctg cactttcacg accttcgaca
    45361 tacgttcgcg acgagattgc agcgtatcgg tgtggattac gaagtccggc aggcgcttct
    45421 gggccatcgc atgcccggca tgacggcgaa ctattcgcat gggggcccgg agtgggatcg
    45481 gaagctgaga ttagcggtgg aaggactcga aaaagcctat ccgttgtcct atggattgtc
    45541 ctatgaggcc aaggcggcag tggggcaaac tgcagaagtc attgatttga atggtgagtc
    45601 ggctgggatt cgaacccagg accctcgcct taaaagggcg atgactcgca ggtcatttta
    45661 cctcctcgca tcctaaaaaa cccagtaaaa cgatagttct tgccaattaa actcttgtga
    45721 gttaccgtga attattgctt ttatgtggga ttcatagtgg gattagcgag gaggaaccat
    45781 gcgaacagcc accgaataca aactccacaa taagaccaac cgcgaccgac tgaagatccg
    45841 gaagaaaccc tacttcgtga gcttgagtcc cggcttgtgt cttgggtatc tcccccgtgg
    45901 tgggaaagct acgggacgcc caggggtgtg gtacagccgc cggcaagtgg gcgaacgcat
    45961 cattactgat actggatccc actcacgcta caaaatgaaa cagcttggcc aagcggacga
    46021 tctacccggc atgaccgcag atggtgagac catccttgac tatgatcagg cgtgcgtaca
    46081 ggccagagct cacgcagccg aactgatacg cggaccgata aagactcccc tcaccgtccg
    46141 gaaagcgatc gatcgatatc tgacttatct cgaacaacgg aatggagaag atgcaaggta
    46201 ccgcaccgaa agcaaattca ataagtgggt gttgaagcat ccgattgcag atgaggaagt
    46261 gaacgccctc accctctcac agttgcaaaa ctggcaagcc tctacagtga tacgcagcga
    46321 agacgatccg gagcaagaac gacggagcca agacacttca aaccgcgtcc ttcggagcct
    46381 caaggccgct ttgaatcact gctatgacca tcgggcggaa accggcgtgg tcaacaaaga
    46441 cgcgtggggc cgatcactca ggcaatttaa agacgtgggt actcagcggg agtatcattt
    46501 cacagttcct gctctgcttc ggctaatcga taaagccgag tcgtactttg ctcaagtctt
    46561 gaaagcggca ttctatacag gagcacggcc aggagaattg cggaaactca gagcgaaaga
    46621 tttcaaacca tcgctaaaac aactctccat tgccaaaggc aaaacgaaac aacgcgtgac
    46681 gacgttgacc gctgaagcgg tggcgttctt ctcagccctc gccaaagata aaacccctga
    46741 cgcgttcctc ttaacaaaat ccgatggcac cccatggctt cccggggaac ataaactgcc
    46801 catgcgccat gcgctcgtcg gcgccgagat cttcaccgcg aagcaactcg acgacatgcc
    46861 acgacatgaa cgacccgtcg tctacagcct gagacacacc tatatcagcc gagccattga
    46921 gcaaggcatt ccgatgttga tcattgcgca aaatgttggc aacagcgcgg gtatgatcga
    46981 gaagcattat gcgaaaatca tcgaacggac gcggcaagag tggattgaaa agggagcccc
    47041 gagcctctat gccacagcct gagacgaagc cgacccgttc aaaaaaactt ttattagtgg
    47101 agtcgccatc tgaagaatac tacgcggcga tgactcctga cgaacgtgcc gagtacgagg
    47161 cagacccctc cgtcgaagac cttgcgacat ggcggcaaca ggatcagcag gtactcgcct
    47221 tagtcgcttt cggaggatta gttccctcgc ccgtagctga caaacagcag aagtggctga
    47281 agagcgtgga gggtcgaagc aaaggggcta tttcaaaaaa caaagagctc cgagagaccg
    47341 aacaggacgc cgacgaggcc atcctcaatt acctgaagga gtgtccactc cctcatgggc
    47401 gcgttacgaa aggcggagca ggcaagtggt tttataagca caccttgtgg cctaagtctt
    47461 ggccgaaggc gtccgagaat ccaggaaaag gccgccgcag gcctcctaca tttgcgaaag
    47521 ataaacatgg tcagccgact aaggaattga ttgttcaatg tccctcgcct aatgtcatca
    47581 gcagagaatt tctcccgctc ctcgaagaac attttaaatg tcagctatac cgtcgtccca
    47641 cgcctcgcct ctcctctccc acaaaaaaat gacaatatcg taaatagttt gttctgccgc
    47701 caaggacaga acatgccgtt gatgagaggg tttccttacg aaaggagatc ccatcatggc
    47761 cgacgatcaa gcagaagaca ccacctcgga aaacacccct ctcctcgcag acatccgcgc
    47821 cgcacaactc gacacctaca aagcgcaagc acgggccgca atcatgaccc agccggtttt
    47881 cgtcgacgcc gaggttgcgc aagtgctgca cgtgccgttg agcacctatt ttgcgatccg
    47941 acgcaaactc aacattccgt cttttaaaat tggaaaaaag cgattgacgc gccgcgaaga
    48001 tcttttgaaa tggatcaact cgcaagccga ggcgacgcga tgagcacccc tgaagtgagg
    48061 ggcagtacat acgaaaaagc tcaagagctc ttgacgcacg agctctacct ctttccgtcg
    48121 gaacacaaaa agaagcacat caaaggttgg cctgaaaaag catcccgcga tcccaaaaag
    48181 atcaaagaat ggcttgggcc cggaggaacc cacgaatcgt ggaagatatc gatcttcacc
    48241 ggcaaattgg gggacggtaa atccctcgtg gtcgtcgaca atgacccgcg gcacggcggc
    48301 gatgagacga tccgagccat agaggcacag catcccgatg ccttccctcc cacccgtgag
    48361 caccgcacgg cgggtggtgg atcgcatagg ctgtattgga caacatggcc tggcgtgaaa
    48421 tctggtgcgg gtgtcctcgg tccgggtgtt gatattaaga gcgccggcgg actggtgcat
    48481 tttggggacg ggtatgatct catcaacgac ctgcccattg cggaggcgtc caagtatctt
    48541 ctcgcacagt gtaagccggt cggcagtacg agccggcccc cctcacgaga gcaagtgctg
    48601 ccctctgtag ctatcaatca agagcatgcg aatagccgag ccgaatggta cttagcacac
    48661 gacgccaagc cctccataca ggggcagggc ggcgatcaaa acgcgttttt cacggccgct
    48721 aaactgaaag attttggagt tgatgaatcc gcagcgacgc tcttgatgtg ggaattttgg
    48781 aatgaccgct gtgagccgcc gttttatgcc gatgagctgg aaacgaaagt tcggaacgcc
    48841 tatcgatatg gtcaaaatcc tccgggcatt gacgcaccgg aagtcgtctt cgccagatcc
    48901 aaaagtgcaa ttgaagattt taaccgcgat cacgccttag tgacgaccgg tggtggagtg
    48961 gaagtgctgt acaacactct ggatcggcac ggcaacccgg cattcgagca tttagatgtc
    49021 ggcgcctgca agatcaaata cgccgcgcga aaaatcttcg acggaaaaaa ggacgcgccc
    49081 ctctttgatg aatggctgcg aagtccaatt cgcaattcct acgatgggta cgtgttcaag
    49141 ccggggcagc cgggagatgt catcatcgac ggtaacggcc acaccaaaaa gttttttaac
    49201 ctctggcgtc ccccggcctt tgctcctgcc ccgtcagggg ccacccatcc agcggtggat
    49261 cagttccttg aacacagccg ctacaacatg tgccgcggcg ctgaagagct ctttcgatgg
    49321 ctcatcgcat actgcgcgca gctcgttcag cgtccatggg aaaagccgca tgtcgcgctt
    49381 gtattcagag gcggcaaagg ggtggggaaa aatgcgtggg ttgaatgcat tcggtctgtt
    49441 ctcggtcccc attccctagt taccagcgat cgccgctatc tcatgggcaa tttcaacgcc
    49501 cacctcgaaa actgtttgct gttggtattc gacgaggcct tctggtccgg ggataagcag
    49561 attgaaggca tggtcaaaaa cctcatcacc gggactgaac atatcattga gcggaaaggc
    49621 aaagagtcgt acgccgtcga caacctaaca cgcgtcgtca tcatcggtaa tgaagactgg
    49681 ctgtttcctg cctctccgga tgaacgccgc attgcattct tcgatgtagg agaccgccgc
    49741 aaacaagatc acccgttttt caaatctatg ttggaaggca tgaaaagcgg aggcgctgcg
    49801 gtcttgtggc gctatctgat ggactacgac atcagcggca tcaacttgaa tttggccccg
    49861 gttacgtcgg gcttattgga tcaaaaactg ttgagcaacg aaccatggtt ggaatattgg
    49921 cgcggctgtc ttcgtgacgg ccagatcgcg gggtcggagt ttgaaggatg gccgtcatcc
    49981 atcgactgta aacgattcca gatggcgatg agacaagcac ttaacggccg caacatcaag
    50041 gggtggatac cctcggaaga gtcgatcggc aggaagatga agccgttagg gttacgacga
    50101 gatcggcaac gagtgcgacg acaagagcga agccccgaag gccccgagtt tgtctacgtc
    50161 tatcacattc cagagttgcc cgaggtgcga cggttatttg atgtctttat ggggcaaccc
    50221 acgcaatggg aggcagaaga atgacgggga tcatctccca cgtcgttgta cccagtgttc
    50281 ccggttgtgc ctggtacgtt ttcaaccagg aacagcgaaa ctcagctctg ggagaggctt
    50341 gtgcctatgt tcctagtgtg cctggtattt taagccaagt cgctacagaa ctgggactat
    50401 acagccatgg ggctgcggtg cagcggggta tatcaggcat atataaatat ttagtgactt
    50461 ataaaaaaac taagaacact aggcacacta ggcacaatcc gttctggaag cggtgtttcg
    50521 ctgttcctag ttcccgcatg gcactaggaa caccgggccg aaaccaggaa cagcgatggc
    50581 aagggtgtca tttttgcaac agcctgctta caggtaatgg cggtgctctc tcatgcaagg
    50641 gtgttacagc ctccgatggc cctatttccc agaatctccc ttcaagatgg ctgatcagcc
    50701 cccggaccca cacttcgatt agcaccagga tcaacagcga caacgtcctt gttagccgtc
    50761 atagggctat gaaatgccgc tccagagcag cgactaagac tctcgaccca ctatctctcc
    50821 cacattgcag ggtggaacct gaggcgtatc aagtgacgaa ccctggaaat gaagagcata
    50881 cgcagatcga acgcccaatg aatcgacgat tccttttgag taggggggta gcatcggcgc
    50941 ccacccccct tttccgagtt atccgcgccg ctagcaggac cctcaagatc ggtcgaccga
    51001 tctttttcaa tcgcccgctc gcaatttttg atcgggcctt aaattttcta aatatttttt
    51061 ccgtgtccgc aacccacaac cccaacgaaa aggaggagtt atgtcagacg acgatgcact
    51121 atctgccatc gaggaagaca accgaatggc cgaagcgcga gccctcgccg gactcaatgc
    51181 cgccggtgat gccttactcg ccaagcctag gtccccaacg gcaaaacgga attaccggaa
    51241 tgcccaggcg aaactggcgg atgttcgact gttcaatgaa caacgaatcg aacacctcac
    51301 agcatcgaaa aattagagga gatggaacca tggcacttct gcaaacaatc aaaacaaaaa
    51361 tgatgctctc cgacatggat aagttaggca acctcagggc acagcttaaa gctgcagaat
    51421 cggaccggga cgccgctcgc gcggcactcg gcgcggcgct cctagacgag gtacccccgg
    51481 cggcggctga cgcgacggca caaatcgagg ccgccgacaa acgcttccga gacttgacgg
    51541 ccgcaatcga tacgatcagc cggcgaatcc aggtggccgc cgatcgaaaa aaggaagacg
    51601 cgaaagcggc acaacgcgag gttcttcggt ccgccctagc gaaggtccaa tcagatgcca
    51661 gagccgtgga acacgatttc gagacgttgc accagcacac gaaagcactt caagaatcgg
    51721 tgaacgaagc gttatcggct gccgccgtcc ttggcgatga tgcgcggcat agtttgctgt
    51781 caaacgggcg ggccaaattc aaattctatt tgctgcatca cgcgtccgtg atgccgggtt
    51841 gcaaaacgcc gtacatcaaa agtcttgagc gctactcgga aaactttcca aaaccagcgg
    51901 agctcaagta accattcacg cacgaagaga aaggaacact aatcatggac gaacccacat
    51961 tgaacacgac accgaatccc gccacatccg accctgctcc caatggtcaa gatgccccaa
    52021 gcccggaaca ggcgacggtg gccaactggc tccgtgaaga tttcaacgcg ggcaaaatga
    52081 cggccgacca gtttgcggag gccatgcagg agttgacggg ccaggaggat acgcaggggc
    52141 tggatatgcg caccccggaa caaaaggact atgacgccag ctttccgccg gcgcgcgctg
    52201 aggactttaa ctttccgttt cccgaacatg gtgtatccat gaccgccgac atgaaagccg
    52261 ccgatacgat ggcgcgcgga tggttggcaa cgggactctt taccaaagag cacggatcgg
    52321 ccctagccgt tgaagtcgac cgtgtggcgc ggaagatcga gcacttcacc cccgcggagc
    52381 atgaactctt cgcgaggtcc gagcggctcg tcctagaaaa agtatggggc gcagacacac
    52441 aaaagaacct gtcgattgcc aaggccttcg ttcgagaggt ggccgagaaa catccgcaga
    52501 tcattgattt cctcgatcag actggtttgg ggaattcgtc gggggtgatt atcgggctgt
    52561 atcaccaagc cgaacggcta gcggcccgga atggcgaatc gctatgaaaa cgtcgcagga
    52621 gatgaaagaa gcgcgctatg cggcaggcat tatcaagggc ctcaccaaaa agcaggccgc
    52681 cattgaggcg gggttctctg tctctgtcgc ggagcattcg gtgaacaaga tccatgagaa
    52741 gcccgcggtg caggcggcca ttgagcaagc ccggacggaa atccgggcgg ccgccgtcta
    52801 cgatctgaca cgagccatgc gggaatctct tgaggtaatc gagttcgcga agcagcacaa
    52861 gaacgccatg gcgtacttta aggcggtgga acatcgcgcg aaactgtcag gcttgctaat
    52921 cgatcggctg gacgtgaagg ccgtggtagt caatgtccgg gcggctcttg aggaggcgag
    52981 aggccgccta caggcctgga atggtccaag gttagaggca tctcaatatg tgaatccgct
    53041 ttctgattag gagcgtttta tgaaataccg caagaataca tagcgctccg ctcgaaagag
    53101 ggtacagagc ttgtggagtc gcgggctcgt aaagcgtccg aacttgccac actcacctaa
    53161 tgatacttga gcaattgtta gtaaatcacc gggctatgta gaagaattac ctggcggtaa
    53221 caggaattga tttgcgcaaa tgctgcgatt aggatgcgcc gacggaagta tttacaccgt
    53281 acaagggaaa ctaattttcg cgatatttgc ctgagagaaa tagtggagca cgaaatgcag
    53341 aagttaaagg ttaatttaga acattgctat gggatagaac aactttctta tgaattcgat
    53401 ttttctacga aaaaacgttt catcatatat gccgcaaatg gcacgatgaa gacttcgttt
    53461 gcaaacacct ttgagaggct cagcaaagga ttaagtccag aagaacgggt ccacggtaat
    53521 gcttccgtat gctctgtcac cgacgatcac gatgtgcctc ttagacctga agaaatattt
    53581 gtaattgttc caattgatcc tgagtttaag tctgagaaaa tgtccacatt gcttgttaaa
    53641 aaggagctca aggatagata cgacgctatc cgaacagttc tcgaaacgaa gaaaacagat
    53701 ttgattaagg ctttgaagac cctttccgat cttccgataa acgcggtcga agcaactgtc
    53761 gccaaagaca tcgcatttaa tgaaagcgat ttttacggtg ctatggaacg tcttaggcct
    53821 aatgtcacag ccaagaggaa aagcaagctt gggccagttt tgtatcaatc aatttttgaa
    53881 gagcgcgttg ttaagctgat cgaaagcagc gatttccagt caaagatatc gcagtacatg
    53941 aaagattatg aggatcttat aagcggatca cgctttttca agttaggtat ttttaaccat
    54001 gataatgctg aagaaatagc agacgccctc gcaaaaaatg gattctttga agcgagacat
    54061 tcaatcaata tgatggcaag agccggaact gaccgtgagc cacccattga aacaaaggat
    54121 aagctcgttc aagtcattcg agatgagaag cgcagaattc tctcagatcc aaagctgacc
    54181 caaaccttcg actccattaa tttaaagttc acgaagaatg agtcaacacg caatttccgc
    54241 cgattgctag aaagcaaccc cgctctaatt gctgaactca ctaatctgcc gcagttgcgc
    54301 gataagctat ggaccgacta cttaattgaa agaacagaaa tctacaacga ctttatgacg
    54361 gtatacgatc aaacgaaaga tgaaattcaa aggatatttc aggaagctag agaggaggaa
    54421 acagaatggc gcaaggtact ttcggtattt agaactaggt tttcggttcc attcgacgcc
    54481 acgatagaaa atcaggtgga cgcaatgctt catgaaaaag cacccgctct tagctttacc
    54541 cataccggga aaagtctaaa gactgatgat ttgtggaagt gcttaagtat gggagaaagg
    54601 agagcattat acctactaaa cattattttt gaggtgaggg cgagaaagga actgtcccac
    54661 aaaactctgt atgtgattga cgacattgcg gactcctttg actataagaa caaatacgcg
    54721 atagtcgaat atgcgaaatg tcagaattcg aaaattctta tctgcttctg ttcacgcaca
    54781 atttcgattt ttaccgtacc ttggtgagcc gctttgacgt gggtgagaat aaagcgtttg
    54841 tcgaaaaagt tactaatggg ttaaaaatta taaaagacaa atattggagc tctccatttc
    54901 ttaagtggca ggaacaaatg acagagaata atgagatgtt agttgccaca ataccttttg
    54961 tcagaaatgt agcggaattc tgtggtcacc acgacatttt tgatgcgctc acgcaatttt
    55021 tacatttcaa gccagataca cacaccaaaa cagttgctga tctccaagat ctttttaaca
    55081 aggtcttagc aaatccagtt gccctaaggg actctggcac aagcgttaaa tcgctcatct
    55141 attccctggc agaagcaatt attactgatc cggcagaggt agtatcactg gaaaagaaga
    55201 ttgttctgtc gatcgcgata agaatgaagg ccgaagaatt catggttgga aaaattgagg
    55261 atgatgcgtt ttggcgcgct attcaaaaga atcagactgt aaagctaatc aaaaagttta
    55321 aagcaaaatt tgctaatgat ccggctcaaa aggaagcttt gggtgttctg acagatgtca
    55381 atctgatgac tccggaaagc atacatgtaa attcatttat gtacgaacct ctcctcgata
    55441 tgtcgggctg gcacttgcag gaactttaca ggcgtgttgg tcggttgcgt tgatagttgc
    55501 ttcaattgcc ttgtttcaca cgtgcgatag gtctagcgat cgatctgtgt tgcaagatac
    55561 tgccagccct ctatgtggct aaatcacacc catcctaaag cttcgaaaag ctgcgcaagt
    55621 tgacaacata gaagtagctg taaaagagcc gtttctttgt accttcgtca cgcattccta
    55681 ttcctcaacg gacggtcaag catgccctaa gcaaaagtat agtaggggcc tcattgagat
    55741 atcaccaatt gaggtttata cttccgccgc ccctcggcaa tagatcacta gacacttgct
    55801 tgtattccta taggaattga gtcgctatga accgtaacct ccggtggttg cattgctcgg
    55861 tatttttgcg tgcggattaa ccagttgcag tatcgacgcg caaggtaatc gagtggtcgg
    55921 cacgccgggc ctcgcagccg cgggcttata cacttaaacg actccggaga tcgaggcaaa
    55981 aagccactca gagatcatcg agaaatatct ggctaagcga gacttgatgc ctatcgaggg
    56041 cgtgtggata gtaggctcga atttctatga agtggcgatc atccgcaaca cgacggagca
    56101 attaaggact acgattatag gcgtcattgc agagtcgcga acagagtcac gggccaaggg
    56161 agacattgta ctcatcttaa ggaaaccgcc tctgctgaag cctatggcgg gtcttacttc
    56221 gatttggaac acaatgaaac tggaaccgat tctgccctca cctaaccctc ttgaagttac
    56281 aattgctgat aggcttggcg ggcaacatcg aactgccttg gttcgcaatt atcccaagga
    56341 atcccgcacg cggttcctga cacgctctgg gagcagtacg gaaactgata ttttatcgct
    56401 gcaaggtcgg ttctatcgaa ctgaatgcgg acatcttttt gaagtatgta cagaatgata
    56461 tatcactgct tcggattgat gtttaaaaac ttcaagattc taccgtccat tatggaacgt
    56521 gctttgaaat cggcaagtca gactctgtga gatccgggga ttctgtattc gccattggct
    56581 atccgcttct ggactcttgg ccgcttcccc cagtattggc cacgggctag gcagcaatgt
    56641 gtgagtcatc gataacgatc cccgagtgtt tcaaatcaga attccaattc aggtgggaaa
    56701 caccagtcgg gacacgtcat aggcgtcgcg aacaacctga acgcagtgcc aatatctcac
    56761 aagtatttgg gctataatcg cccgccatgc caaaccgaag acaaagacga agacaaaaaa
    56821 ccgcgaccat caccaatcct gcaccgaaca acccttcctc ggaaaagaaa ccgaccgatt
    56881 ggcgttctct gaagctcata aataagatag tcgcaataat catactgctt ctaagcggcg
    56941 gaggaggtac gtattatatc gtttcaaaaa ttaacctcac tggccagcaa caaaccgtga
    57001 ctaactcatc gggtgttgtg cagcagcaag cagacgggga tattattaac catatctata
    57061 atttggatag cgagactctc gcaaaagcgt ttgcaaaaag tcgcggcctg caaggtggca
    57121 gcgaagtgag aaaacatgag gaggatctag aagaaagtgt cgataaaaaa gcggatgaaa
    57181 aattgcagca gattaaagaa gccatcgacg ccctcaaaga attggctaaa gccagcgccc
    57241 cgcgcaagca acccgccata aaagacgctc tcgaacaact gaaagatggt aatgctgact
    57301 tagccatgca aacatttcag gaagtgttgg atcaacaaaa gagtgtgggg aagtcagcgt
    57361 ataaggaggc agcagcggct gcgatgcata ttgggcactt ggcaagcgcg acaaacagtg
    57421 aaaaggcaat acccgcattt agacaagcga cggagctgga gccggacagt tccgaagcct
    57481 ggaatcaact tgcgaatgca cttcaacgaa tggacaaaaa cgacgaagcc gttgatgcgc
    57541 taaagaaagt gttagctttt gcacagcaga aaaaagatct atcgtggcaa gctataacac
    57601 accacaatct agggatagtg tacgcgaatc agggtaaata cgaggatgcc gaagagatgt
    57661 atctaagggc tcttaatatc aaccgagagc taggtcgcaa gtccggcgtc gcttacaact
    57721 acaactcttt agcattatta aatgggcaga aggggaaaaa ttacatcggc gaggattatg
    57781 cgctagatag cgtaaatact tacagtgaaa ttcgtcacct tacggatatg gagtatgccg
    57841 ggctgggcga ctctcttggg acgttagccc attttcacgc gattcgaaaa gattttaggg
    57901 gtgcagagaa cctttactgt gagtcgtttg ctatatacaa gaaactgaaa aatgattata
    57961 gagcggctac cgtgtatcaa aacttggggc ttttgtacca aaatgagggg caactagtcc
    58021 gagcagcctc gttatttaag agaagtgccg ttcttgctcg cagtgtcaat aacactgggc
    58081 aaacgcagag agcgcgctct agcctagcgg cgctcccggc tgatgtggtg gcaaaagctg
    58141 cggataatag tgaaatctgc aactcatcca ctaataagaa atcggctagc aagtagtttt
    58201 ttagcctctt cccccctctt agattccctg ccgtgaacag gatgtttctg acacgagaac
    58261 agttacagat ggggcgactc ttggaattca ttagacagat ctgtttgtaa ccagcacgac
    58321 cggcggcctg ccggggactg acgcgtacgt gacggaaaca ggtggaactt ccatcggcaa
    58381 ccagggtaat aggtatgcat tctcgaaacc gccgattaag agccagttcg ctcattgctg
    58441 ttgcattggc tccttcgttc ttcatcggat gcggaacctt acaactagat cgcaatatca
    58501 accagtacca taggccttca aggtcttctg ttgtggatca ccgcgaaccg acacgcccgc
    58561 catttgtcgg tgtggccatc tcaggcggtg ggagtcgctc tgcaaacttc gcagctgctg
    58621 taatggagga gttggactat tacggtttcc tggatcatgt caatgcgata tccgccgtgt
    58681 ctggtggaac gttgcctgct gcctattatg tcttaaaacg taaaaagcct gactggaatt
    58741 ggggcaacct gaggagcagg gttggagcaa atatatattc acgtttatta cttaaacaca
    58801 taaatccatg gaagctgctg aactatgctt tcactgaata caatcgatcc gacatgatgg
    58861 cggaggtgtt cgacgatcta ttgctagata atgctacctt tggagatatc gatcccagca
    58921 tgccccaact cttaatcaat gccacagaaa taaacggagc gtcattcgta ttcagtacag
    58981 agatgtctta taaccttcgt tcgtcgtttg ctgattaccc aatctcacgt gcactggcag
    59041 cctctacctc ggttcctgga atctttcaag gaatcgccat tcgtacatac caaaccgaag
    59101 acaatttttc ctatcttcat ttagttgatg ggggtatcac cgaaaacctt gggctggaga
    59161 acttgctcca acattacatg gacgaagaga acactgcggc tcacccattg cgcaaagttc
    59221 ctcctcgggc ctgtttcata tttctaattg atgcatcggc aagcggaagt gaagctcagc
    59281 gagtagcctc gtatcaacgg cgagatttga gaaattggag tgatttcatt gttgatcgta
    59341 atctgatgaa tgcatttgat gtcatgtttg gtcgtgctca ctcacagttc atctccagac
    59401 ttcgcggaag gctggacgat gatgctgtct ttgaatcgcc aatccaatta aattatggca
    59461 gggtgaactg caccatttgg caatttcacc ctcgtttctt gatatatcat ggtcttcgtt
    59521 ttgataatcc agacaattat cctatcgcag ctcagttgtc cgcaatactc tctcaaatcg
    59581 aaactcattg gaaactgtct tcccccacct cctgtagcag cgccgcatta caggcggccc
    59641 tgtacgacgg agcaagaatg ctcgtttcag cggaccgtgg tgccctcaac accgcaatca
    59701 cttggtttgc ctcccatgat ttacggatga aacaggatgt tccacgatca agcccctacg
    59761 aattcttctc aaaacatgtg tcagccgtga caagcgaaaa caatgtctac aacggctcag
    59821 gggtatatgg tcaggttttc tgtgaacgtg gagattgaag tgcgttagca gggattcgcc
    59881 cgctgaggct aggcgaaaag attcggatta ttattaataa actgtatggg ggagcagggc
    59941 ttccggatga ttcatcgagc tcattaattg tcagtgtcgc tagtgcgact aggccgaggc
    60001 tctgggtggg gcttacgatc gctctggaat tgcccccaat ctccgttata acgccatgat
    60061 tttgctatat gactgctatc gtacacgaat aaaacactgt gcctccatga agaagattcg
    60121 ccatatcccc cataggcgcc ataatatcct tcgccacgga ccccttcctt agaccactca
    60181 caaacttctt cacgggattc aaggagaaaa caccggttag gcggtccagc agctttaata
    60241 cgttcgtctc tatgtttgcc gatccattga ttaaactttc cggagagctc gaagtaataa
    60301 tcttcatctc tttctgcaga ggttttacat ccagctatct ctaaagcaac gatagccatt
    60361 gccatgagca gaaaaacctt aggcgcgaat cggatcatag cgacaacaat tatgccgaac
    60421 gagtgaaacg gagtctcctg ctaacagtgt cactttagta atcgatctta tagaacattt
    60481 ccactcagat tctcaatgcg gagtcctaca aatagaatgg ttgcagagac cgatcgcata
    60541 gccggtctta ccactcgcga cagcagtatt tctcgctctc cgtcggcaca cgcagccata
    60601 atgggcaaaa cgattggcca agtccgtact ctagtccggc cgctcgtgaa agggtcgcgg
    60661 acgaccaatt tgggtggaca agctgtctgc ctcctattcg cccacgtatc gaacagcaag
    60721 acattttccg gttgcggtaa agtggcggga ctgtaacagc tcagcatcgt tgtctcttgc
    60781 gatgggttta taggttcgct cgacagagtg aatttccgca acgaagacat gcgtgtcatg
    60841 caaacccccg ctgtgatcgc tgttaacttg gtctgctatc cgcttagact ccggcctgtg
    60901 gtcgttgaag acctcacatc tcagcttaaa actcacgctg atactaccaa gcccaaagct
    60961 ggccgacaca tcctccagca tgccccgctt gaaatggatt aggtattgtc cggcctcaag
    61021 ttctacatca tcgacgtatc ccgttcctat aatcggtcta ttggggatca gccgtgcttg
    61081 tacgtcgata gtccagactg gtcgacgcgt gaatgcctct ttccacgtag catcggcgct
    61141 gtagacttcc tgaaattcct gtgtcgcatg aaggatccgt tcttttgacc tcgcgcgtga
    61201 ttgttcgcgc tctttcgcct gttcattttg tgacgaagga gggtttttgc ccttgcaggc
    61261 cacagggaac atcatcagtg gccctagcag cagaagccga aacagagggg aagcaaaggt
    61321 ggcgacgtaa cgatgaagtg aagagaccct actcatccga taacttgcga acgggatgct
    61381 aagtgggttc gatagtggga ttttgaattc taccgcttta cagcttcacc aataaatcaa
    61441 tctaactaat tgatttaatt ggtgagtcgg ctgggattcg aacccaggac cctcgcctta
    61501 aaagggcgat gctctaccag ctgagctacc gactctccaa aacataacaa cggcacaccg
    61561 ccacgtgtgg cgcgggacgg agggggatca taccactgac acgtgatggt tcgctacggc
    61621 gagtgatgcg ttcgacgaac aattcgggcc ctaccgtgac cgtgtcgagc gcacacggcg
    61681 gcctgccttg agcggattgt tgaggatgat cgtctcatcg cgccgcgagc cggtggaaat
    61741 catatcgatc cggcactcag cgagctcttc gatcctcgtg aggtaccgct tcgcttccgc
    61801 gggtaaatcc ttgtaggtcg tgaccccagt ggtgggagca ctccagccac gcacccgctc
    61861 atacacaggt tcacaattgg tcaacgccag cagatccgac ggcatctcac ggtggatttt
    61921 gccgttcacc cgataccccg tacagacttt cagttccttg cagccatcca atacatctaa
    61981 cttcgtcacg gccaacgaag agagcccgtt cactctggtg gcatgacgga cgatcacgct
    62041 atcgaaccac ccgcagcggc gagcccgccc ggtggtggcg ccgaattctt ttccacgctc
    62101 ctgcagccaa ctccccacct catcggtcag ttcagtcggg aacggtccgc taccgacacg
    62161 cgtggtatag gccttggtga tgcccatcac ggcgtcgatt ttcgtcggcc ccactccggt
    62221 gccggtacag gcgccgccgg ccgatgaact cgaggaggtc acatagggat aggtgccgaa
    62281 atcgacgtcc aagtgcgtgc cctgcgcacc ctcgaacagc acggtcttgc cggcatcgac
    62341 ggctttattc acgacgaggg ccgtatcgat gatgtagctt ttcaaacgat cggcataccc
    62401 catgtactgc tggtagacct tttccaattc gaaacagtcg actttgtgca gctgctcgag
    62461 cagccaattg atatcgacca gattttcttc cagcttctgt ttgaagagcg ggggattgag
    62521 caaatcgccc atgcgaatgc cgatccgcgc catcttgtcg ccatacgacg gcccgatccc
    62581 gcgccccgtg gtgccgatcc gacgaacgcc cttcgactgt tcagacgcct tatcgatcgc
    62641 cttatggtaa ggcaggatca gatgggcccg gtcgctgacc gcaaaattct tcccgatctt
    62701 cacgccctgc ccctgcagat gatccatttc ttcaatcaac gccccgggat cgaccacgac
    62761 gccgttgccg atcaggcagc gggtgccgcg atacagaatg ccggagggga tgagatggaa
    62821 aatgaacgta tcccgcttat tgatgaccgt atggccggca ttggacccac cctgataccg
    62881 cacgaccata tcggcatcct ttgcgaggat atcgactatc ttccctttgc cttcgtcgcc
    62941 ccattgggag ccgatgatga cgagattcgc cattgttcgc tgcctacgct ccttctgccc
    63001 ttgctgaact cgaaaaaaaa gccctgacct cgtactgatc agagccggaa gggcatcatg
    63061 gtaggattcg cctcgcggtt tgtcaagcaa gcttttcagc aaggatctgc tatccgtcgg
    63121 cagacacgag gtgctcgccc tccttttcgt gcccccatcc cacggagttt ttgcagccgc
    63181 ccggcgcacg agcaaaaaaa tgaggacagt ccagggcctc actccaatgc ttttcttgac
    63241 tgacaaaaac cgcccatgct agattgcaga gttacgtctg cacgttgatt ctttttcccg
    63301 tgcctgtgca catgtacaga tgagcttgcg tggggctgtg gcgcagttgg gagcgcgcgt
    63361 gaatggcatt cacgaggtcg ccggttcaat cccggccagc tccaccaaat aaagcttgtc
    63421 cgaccaacgc ttcgaatgtg tgcggggata tccgaactct acgagtcata cgcttggccg
    63481 gccgccggtg cacacacgga cgctgacact ccgccgcggg tacctcaccg aacgtttcac
    63541 cccttacacc gagtcttact gatccatgct gcagcttgaa tctgtccata aacaatttgc
    63601 taccaaagtg ctgctcgaag gtgccacggc acacctgcgt cccggtgccc gtgtcggcct
    63661 cgtgggcccg aacggcgctg gaaaaacgac gctcttccgg atgattctgg gcgaggagtc
    63721 tcccgataaa ggcagcatcc gcaaacgccc gcgcctccgt gtcggctacc tcccgcagga
    63781 actggaaacc attgtcggaa aaaccgtcct ggatgccacg caccgcgacc tatatccgga
    63841 acatgaagcc gaacggattc tcgccggcct gggcttctcg gaagccgatt tttcgcgacc
    63901 gattgagaat ctttccggcg gataccgcat gcgcgtcgcg ctcgcgcact tgctgctgtc
    63961 gaaccccgac gtgctcatgc tggacgagcc gacgaaccac ttggacaagc cgacccagcg
    64021 ttggttcgag cgatttctcc tcgactcgaa tctcacgctc ctcgtcatct cgcacgacac
    64081 caaatttctc gacggcatcg ccacgcatat ctgggaactc cgcgatcgca ccattcagga
    64141 attccgcggc aactatacga agttccagga actccgggcg gcgcgagacg cccagatcga
    64201 atcggccgcc aaccggcaga gcaaagaagt ggctcgcgtc cagaacttca tcgatcgctt
    64261 ccgctatcag gccaacaaag ccaagcaggt gcagtcgcgc atcaagcagt tggataaggt
    64321 caagttaatc gagcgacaac gcgacaccaa acgcgtgcgc ttcaaattcc ctcttccgtc
    64381 ggccagcggt cggcacgtgc tggaactcaa aggcgtcgcg aagagctacg gcgaaaaggt
    64441 gatttaccgc tcgctggatt tcacagtgga gcgaggtcaa cggatcgcgc tggtgggtga
    64501 gaatggcgcc gggaaaagca cgctgttgaa aatattggcc ggggtgctgc cgtttgaaaa
    64561 gggcacgcgc caggtcgggc acggagtcac gcttcactat tttgcccaac atcaggcgga
    64621 gtcattggac ccggacgaca cgatcttgga gtcactggcg gaggtctccg cgcaggccga
    64681 aacgaacttt ctccgcggta ttgccggtgc cttcctgttt tccggtgacg accagaaaaa
    64741 gccgatcaaa gccctcagcg gcggcgagcg caatcgcgtg gccttggccc gcatgctggt
    64801 ggagccggca aacacgttgc tgctcgacga accgaccaac cacctggacc cggcctcggt
    64861 ggacatgctg accgacgctc tgacggactt tccgggaacg atcatcttca tttcccacga
    64921 tccgacgttt ctgacgcgcg tcgccacgct gatcgttgaa gtggatgaag gccaggcgaa
    64981 gaattatctc ggcgactatg agtacttttt gtggaagaag gcgcaggagt tcgaatcgat
    65041 caaggaaagc agcgcggagc tcgccgccgc ccaagcgggc aaatcagacg gccccaccaa
    65101 ggccatggcc tcgcaggtgc aacaaaaatc tcagggcggg gaacgacggg atctgaacaa
    65161 aacgcaggcg cgccttgaaa agcaggtgtc gcgggcggag tccgagatcg cctcgatgga
    65221 aaccaaagtg aaagcgcgag agcttgagct ggccgacccg gccctctatc aggaacttgg
    65281 ccgatggagc gaattgcagc gagagcagga aggttggaaa aaagaactgg agcgactcac
    65341 tgcgcgttgg gaatcgttgt ccgatgaatt gcagggcgtg cgggaaaaac tgacggcaat
    65401 cggttaatcg gtcgacttca gcgtctcttc aagatagtag tacaaccagc ggttcttctc
    65461 tttcgtcacc agatcatccc acaccacccc cagcaatatt cccttctccc acggtttggc
    65521 ccaagccacg gtggcgttca gtttctcctg ttgctccacg cgatccgggt ccaggaaggc
    65581 cagcgacacg gcaatctttg cgccgatggg cagggtctct ttggaatgaa ggccggcacc
    65641 gatcttgttg acgttgttca gcaccgccgt aaaacccttg gctccgcttt gcggcgacac
    65701 cagggccgac ccgacgagcg tggcccggac atacttccgg cgctcgctca cactctccgc
    65761 tgaactggat tgacgtgcca tgatgacctc ggccctaagt ataaccgtct gtccaggttt
    65821 gtccagcccc tttcttcatc gaattagggg gttctggggc ggtaataccg tgtgccacga
    65881 agcgactccg ggaggtgatt ctgatcggtt ttggcggctg gatcgtcatg cacataccga
    65941 taccccttcc cgtacccgat gtctttcatg agagatgtca cggcattgcg cagatgcaac
    66001 ggcacgccga gattgccatg ttggcgcgca tcccgcaccg cctcctgcag gcccacatag
    66061 gaggcattat ccttaggccg cgaggccagg tacgtcgtcc cgtgcgccag gttgatttga
    66121 gcctccggca gcccgacgaa ctccaccgcc tgcgctaccg cgttggccat caccagggcg
    66181 atcggatcgg cattgccgat atcttctgaa gcaaaaatca ccatccgtcg ggcaatgaat
    66241 cgaggattct ccccaccttc caacatacgg gccagccaat agagggcgcc gtccgggtcg
    66301 gagtcgcgca ggctcttgat atacgccgac accaggttgt agtgctcctc gcccgacttg
    66361 tcgtaacgaa gcgccttttt gagcagcgca gcctccagca cccgttcatc gatgatccgt
    66421 gatccatccg gcccgacagg cgcctggctg gccacaaact ccactgtcgt caacaggctt
    66481 ctggcatcac cgttgccgaa ggcaatcagc cgctcgcggg cagcggggtg caacgtgatc
    66541 cggagcgacc cgagtccccg ttccgaatcg gccactgccc ggtccagaat ggaaccgagc
    66601 gactcctctg tcagcggctt caacagcacc acgatggagc gagagagtaa gggcgcgatc
    66661 agttcgaagg aaggattttc tgtggtcgcc ccgatgagca ccaccgtccc ccgttcgaca
    66721 tgcggtaaaa aggcatcctg ctgggcttta ttgaaccggt gaatttcgtc gacgaacagc
    66781 gtggttgccc gacccatcgc ccgacgatgc tccgcagcct taataatctc gcgcaactcg
    66841 ggaatgccgc cggtgacggc tgaaaacggc acgaactggg attcggtgtg ctgcgcgacc
    66901 agcccggcca gggttgtttt cccgcaaccc ggcggtcccc agaaaataac ggaggagagt
    66961 tgatcccgct caatggcatt tctgagcggg cgtcccggcc ccaccacctc atcctgcccg
    67021 acgaggtcat cgaagcttcg cggacgcatc cgctcggcca gcggaacttt tcctggacgg
    67081 gtggccgtct gagccggcgc gaagagatcg cctgtggaat catgagagga catggtcgtg
    67141 ctctacctga atactgccct gcattctagc tgtcgcaagg gaatcatcgc aaacgactct
    67201 cgtccccacc tgccgatcct ccccttgatt cccacgcgcg gcaatgctac cacccaccca
    67261 tcggcttttc acaaaggatg gactcatgtt ctgcgacacg aagggcattc ggttggctta
    67321 cgacgaccag ggcacaggac ttccgctggt ctttctccat gcctttccgc tgaatcgttc
    67381 gatgtggacg ccacagacaa ccgcgctgtc cagacaattc aggaccatcg ccgtggatct
    67441 gcgcgggcat ggtgagtcgg atgccccgct gtgggcattt tcactcgaag actacgccga
    67501 tgatgtctgc gcactgcttg accacttggc tattccccag gctgtgttgg tgggattatc
    67561 gatgggcggc tatgtcggct tttcgttttc cagaaaatac gggaaccgcc tgaagggatt
    67621 ggtgctggct gatactcgag cgcaggcgga tagtccggag gggagaacag gacgtttcaa
    67681 tctggcccag actgcctacg ggaaaggaac cgacgccgtg gcggacatca tgctcccaaa
    67741 actactgggc gcaacatcac tgcaacagaa acctgaactc gttgattacg tccggcgcac
    67801 aatccggcac acaccggtaa gcggcatcct cgtcgatctg atggccatgg cgaaccgccc
    67861 cgattccgtc gcccatctcc gcacgctcgc ctgcccaact ctggtggtag tcggccagga
    67921 agaccacact acgcccctcg ccgacgcgca agtgatggcg acagagatac ccggtgcccg
    67981 gctggccgtg atccctgcgg ccgggcactt gagtaatctt gaacaacctg aggtctttaa
    68041 cgatctggtg agaaacttcg tcgaggaact acgggcggcg ggcgattagt ttccgtccct
    68101 gacgctactg cctcgaatca acttcaagaa ctcttcgcgg gtctgttgtt gcgtgcggaa
    68161 cacgcccagc atggaactgc tcacggccac ggtgttctgt ttttcgacgc cccgcatcat
    68221 catgcagaga tgtcgtgctt ccatcaccac cgccacacca tgcgcattca gctttttctt
    68281 cagcgtttcg gcgatctgaa gcgtcaatcg ttcctgaacc tgcaggcgtc ggctgaacgc
    68341 atctaccacc cgaggaatct tgctgagccc cacgactttc ttgttcggca agtaccccac
    68401 atggcacttc ccaaaaaacg gcagcaaatg gtgctcgcac atgctaaaaa aatcgatgtc
    68461 tttcacgatc accatttcgt cgtactcgat cgggaacaaa gccccgttga gcagatgatc
    68521 gatatcctgc cgatatcctt gagtcataaa gcgcatggcc ttcgcaaccc gcttcggcgt
    68581 attgaggagg ccgttacggt cagggtcctc tcccaattcc agcaaaagat tcgtcatcag
    68641 cccttcaatc gggtcctcgt cggtcggtgc cgcagagaca cgcgaccgtc tggctcgggc
    68701 cccagaaacc actttagcca tgcctcactc cctcttctct ccgcttgctc tccacggagc
    68761 cggaatactc gaagtagtta tcccgtgttt caatcacacc gacctttact agccgtcccg
    68821 caggaagctt atccaccaac aaatcccaaa tgagcagcac cagattctct ccggtcgtag
    68881 tctgggtcgc gaactccgga tccaggttca aatcctgatg atcgaaacgt tgcaccacga
    68941 ttcgctcgac cacggcatca agccgtgaca catcgagcac ctgatgcgtg accggatcga
    69001 tatcgccctt cacagacaca aataccacat agttgtgacc gtgcccgttg ggattattgc
    69061 acttcccgaa agtggcccaa ttctcctcag ccgacagctg atcggtatgc agccgatggg
    69121 cagcgcagaa gcgatagcgc cgggtgatgg tggcctgaga catcgagaca actctccaga
    69181 aagatcggca gaacgaacaa gccgggaggg ccccccgcta gagagggagc cactcccggc
    69241 ttgctgctac tcaacggacc attcgcaatc cggttggggg tcaaccggaa ggctcttacg
    69301 cactgaaaga actgccgcac ccgcaggtcg tcttggcttg cggattcttg atcgagaatc
    69361 cggacccctg caagctgtcg acataatcga cttccgcgcc ctgaagcagc ggtgcgcttt
    69421 gagagtccat gatgaccttc acgtcgcctt tttcaatgac cgtatcatcg tcggccattt
    69481 tggattcgaa ggccatgccg tactgatagc cgtggcatcc tccgccgcga acataaatcc
    69541 gcagaccgac cacatctttt tcctcggtca tgagttcttt tattttctgc tcggccaatt
    69601 gcgtaatcgt taccatcgtt ggtcctcctt ctggtctacc tgttcagcac gttacgatac
    69661 tctaccatct cacgggagct tacggaagtc ctgtccctct ttttccaact tcgcctgatg
    69721 aggctgacgt gggttgcacc catttggttt ccggcacacg caccactaca tcccctaaca
    69781 ctcttgcctg gcaacctagc cgatgccaag gctccgacaa ggcttcacga tccaacagat
    69841 cctgttcgtc gaaatcaatc tccgacagat tgtccccacc catctgcact tcaacgcggc
    69901 acgtggtaca ggaggcattg ccgccgcaat cgtgattcag ttcaaacccc agcgtcttgg
    69961 cggcctccaa cagggtgaga tttgattcaa cctcaccgct ccgaccctgg ggatgaagaa
    70021 aactcacacg cggcatccga ccattcctct aggcgctcgc accggcccct gtgcgggccg
    70081 gctgaaaggg acagcgttgc aaacgaatat gctcttcgaa ttcatgctgg aacaatcgca
    70141 aactgctctg caccaggact gcggcaccgg tgataagggt gcaatacccg gtgcccttca
    70201 caaatccaca cagttgcagc aacgaatcga ggtccttctg gctcccttcc ccgcgctcga
    70261 tcttgtccat caacgtcgcc agattgatcg tgcccatccg gcacggagga cattggccgc
    70321 aactttcatt cttgaagaaa ttggagtact tgagggtcgc cgccaccatg catgtggcat
    70381 catcgatcac aatcgtcccg gcggaaccga gtccggtccc ggccttcttc agcgaatcga
    70441 aatccatcgg cagatccagc tgatccgccg tgaccattga gaacgcaggc ccgcctggga
    70501 atactgcctt gatcttgcgc ccgttcggga cacccccgcc gcacgtctcg atgaggtccc
    70561 tgatcgtcac gcccatcggc atttcataca ctccgggccg attcaccgat ccgctaagag
    70621 agaagagcat ggtcccggga cacttctccg tccccacctg agtgaaccat gcggagccgt
    70681 ttttaagaat caggggaatg ttgcacaacg tttcaacgtt gttcaccagg gtcggcttcc
    70741 catacagtcc gaaatcggtg gggtaaaacg gcggcttctg acgcggcatg gccggacgtc
    70801 cctgcatgga ctccaacatg gccgtctctt cacccgctac gtaactgcca tgaccggagt
    70861 agacttcaag gtcgaggtcc acgccagttc cgaggatatt cttccccaat aggccacgtt
    70921 cccgggcttg ggcgatggct ttcctgagat tcgcctcttc ttccgcatac tcatggttga
    70981 cgtaaatata ggaggctttg gcattcacgg ttcgagaggc aatgagacag ccctcgatca
    71041 gttgatgcgg gatatgtttc aggagatgac ggtctttgaa ggtacccggc tcatgttccc
    71101 cggcattgca gacaaagtaa cgctcctgaa ctcgatggtt gaggaccttg tcccacttcg
    71161 taccggtcgg aaagccggcg ccgccacgcc cgcgcaatcc ggctttctta atttcgccga
    71221 cgatgtcgtt gggggtcagc tctttcagac acttccgcca agcttcgtac ccgccggtct
    71281 taaggtaggg gtcgatttcc cagggagccc cttcaagctt ctgcaatacc cttggttgag
    71341 gtagggtcac gttattccct ttcgggggta gcacccccag gttagcagga atgtactata
    71401 cggctgctct ttgggggccg tcaagccaag ttcgcctgat taggcctgaa tctcaatcag
    71461 ttaggcctga taggcctacc gcgaatcagt ccaaagactc gccgacattc gattacggta
    71521 cccggcatcg atcggcccaa gaaaaagggg actggccacc ctcatcggat gaccaatccc
    71581 cgtgattctg ctcgcccgac tgcttacttc aaatggctcc ccgctcccat gaagaacaac
    71641 atgggaatag acagcaagaa gttgatgcga gatgcaatca aggcagtttt tccccactga
    71701 gccatctccg gcggagcggg cgtaccctgc gcagcggcag ccaccgccgc aatgaccttc
    71761 ttctgattgg gccaaataat cccgtgcaca ttccccatca tgatgatacc gagcaaaccg
    71821 ccgatcccca gcgcaagcgc ccccgttcca ccttttgaat agaggtagcc gtagagcacg
    71881 atccccgcga ggacggtgac cgtggcgcca tggcggaacc aattcagcgc ggcaggcatc
    71941 agcttgggaa tgacgatgtt cttcgtcggc ccatccaggc tcttgagaaa gccggcattg
    72001 atgaggttaa agaagtacaa cagcccgatc caggtaatgc cggccaggaa atggatccag
    72061 cgcacgatca agccgaacca gtcggcatcg cccgccccca ctccagccat acccagatag
    72121 acgactatca atccgacagc gagcgcaaat cctgcgccca atgtctgata tggatcttcc
    72181 aaaaatttca tacgcccccc aactgtaacg gtttagtgcc agccccgttc tctactgcag
    72241 gctgctgagc cttgtcaagc aacctgcctc cacacaccct agtcagccga ctaggcagtc
    72301 agcaggcggt cgaccaccgc acataagtcc cgcaccgcgt ccgcagacgc ctgcagtgcg
    72361 gtgcgttcct cagcggtcag ctcatattca acgatggatt ctcctccgct gcgccccaag
    72421 gtcacgggca caccgacgat gacgtccttc aatccatact caccttcgca gagcatggcg
    72481 cagggaacca cccgcttctg gtcgttcatg atcgcctcga ccatatccac agcggaggcg
    72541 gacggtgcgt aaaacgcgct gccggttttg agcagcccca cgatttcagc cccgccgtct
    72601 tgcgtgcgct tgatcaaggc tgccagccgc tccggggaca accgatctgt aattggcttc
    72661 ccagccactg tcgtctgacg caccaacggc accatcgtgt ccccgtggcc acccaacacc
    72721 atcgcttgca cgtccgtgcc cggcacattc aattcttcgg caacgaacga ccgcaagcgc
    72781 gccgtgtcca gcacgccggc catgccgagc acccgtgatt ttggcagccc gctgacctgc
    72841 cgagccacat gcaccatggc atccaacgga ttcgtcacca ggataagaac gatattcggc
    72901 gaacgcgaca ccagttcccg caccaccgtc ttgacgattt tggcgttcgt cgccaaaagc
    72961 tcatcccggc tcatccccgg tttccgtgga atgccggagg tgatgactgc gatagaagat
    73021 cctgcggttt cgtcatatcc gttcgaaccg accacgcgag tgctgtaccc gcagaccggt
    73081 ccggcctggg taatatccaa cgccttgcct tgcgggatgc ccggcacaat gtcgaccagc
    73141 acgacatcgt acgcatccct ttccgccagt cgctgcgcga ccgtcccgcc gacattcccc
    73201 gcgcccacta ccgtaatctt tggtcgtccc atcatcccac ctcgtgaagc gtgaaacgtg
    73261 aagcgtcaag tgcgcgggct ccagcccata cactcaccgg ttacccttca cgcgtgttcg
    73321 tgacccgtcc agccgccgac cttgaaattg atcgtgcaga caaagttatc gccgtcatag
    73381 aaattcactt tgattttctt tttcccataa ttcgccgcga gaaaatcttc gggcgtatcc
    73441 accagcccct ggtaaccgcc agctggatac tcccccaact cgtggaacac cacgatcatg
    73501 cccgttttga cttcttccag aattttagcg gtgcatcgcg gatagatttc ccaaaacttg
    73561 gcgtcgatct gctctcgagt cggctccgga cgttcctcgc cgggcttgaa atcacgattc
    73621 gcaaactttg cctgccgtcg cacataggga tattgatggc ccgtaaacaa cttgtagagc
    73681 cagggcggta tagtcggtcg tggtggctgc tcaatcatcg ttggtgtgct ccaatactga
    73741 aaaggcctct cgcacgaccc ttcaggccgt cagccgatgg taaatcctcg gaagcttctc
    73801 cggcaacccc tccacacgat caatcaccac atagcgcaca tctccataca tccgacgcag
    73861 atagggatct gcctccttat cgatcgtaat gcagaccggc tcaaccccgc tggcgcgagc
    73921 ttcccgcagg gccatgcgtg tatcttccaa cgaatagtcg tccttatagc cgtcatccag
    73981 cggacggccg tcgctgatga ccaccagaat gcgcgttcta gcctgacggg cgagaagctt
    74041 actggtcgca tgccggatgg cagcgccgtc gcgattctgc tgcagaggca caatcccccc
    74101 taacttggca ccggcacgcc cggtcacgag ttcgtcgaat cccttcacga ccacaaaatc
    74161 tacctggttc cgaccctgcc ctgaatagcc gtatacggcg aactgatcgc caatcgccgt
    74221 caacgcttcg cacaggagca cgagcccctg cttttcaatg tcgataatcc gccgcccctg
    74281 ttcgacctgc cgactggtcg atccactgag atccaccaaa aatgcgacgg cgacctcacg
    74341 ttcccgcttt tcacgccgca catagatccg ttccgatggc tcgatgcccg ccgacagatc
    74401 agccatccgc ccgatcacgg catccatgtc cagatcttct ccatccaact gaccggaaat
    74461 gcgccggagc ccgggtgggc gcagactttc gaaatagcga cgcaacagcg tgaccgttcc
    74521 ccgttgggcc gcgagggtct cctccacaaa gtccgatgtc ccttcaatcg cctcgcgctc
    74581 aaccacccgg caccaattca ttctgtagtc ctgaatggca gcatcccact cacggtaacg
    74641 aaccgccttg accgcgccga ctgtttcatc ctcaagacga gccccctcgc cttcaaccgc
    74701 cagaacctcc tccacgagcg agggctgtcg gcggccgggg acaagcacat cttgcgacat
    74761 ctgctgcgaa cgcgcggtgc ccgcgcctga cgaacctccg gcatcaccgg ccaaacccgc
    74821 gccgccttcg ctatcaccct ggccggacgc cgcactcggt tcgggagacg attccgttcg
    74881 gtcgcgcacc agattgggat ccatcgcgcc gcgatagtcc caattatcca tcggccggta
    74941 cgtatcggac aggtcttccg acgacttggg cgccgggatg gattgttccg gagcctccgc
    75001 ttcgtcctgg ctgggaatgg cctcgcgacg aacggctaat aactcgtcca tccgcgcata
    75061 gaggcggtcc gccaggcgca ccgcttcctc ggcggtggct cgaggatggt agatcgtccg
    75121 gcaaagcgac cagagctgat cgacttcgct tttgacggcg tccggaacta tgaccgttcc
    75181 ggcatccgtg gtggagaaca gcaggagctg atcgaccact aattcgcgca ccgtcatgcc
    75241 atgcgcaaga cttctaacgt tcaccgcgtc tttggcaacg gatgccaggt cacggctcaa
    75301 gccgggatac tcggcgcgca acaggtactc aactctggcg tcctccacca gagcccagag
    75361 gtcgcgaatc aggccgggct gcggataccg cgcaaacaca tcgcccaaag tccgggcttc
    75421 attcggcaca gctcgcccat aacgctgcgc aaggtcctca accaggtcct gcaacctgac
    75481 aataggcaac tcgaatgtgc cgaattccag atgtccggcc tcatgcgcca ccatcaccat
    75541 atagagccgc acgttttcat cgtaggtcgg ataccgcctg acgagggacg gcaggccaat
    75601 gctctgcccg tcttcactca ccctggcacg agccatcggc tgggtcgatt cgagagagtc
    75661 gggaagatcg tgaatgcgca gatcgcgacc gcagagcgcc tgcgcgaaca atttgacgcg
    75721 ccgggcgatc tgccgcaacg gtaccccgct catggcctga gacacggcgc ccaaggcctt
    75781 ctccgtttcc atcgcgaagt aggcttgccc cgcctcctgg ctgtactcca acacttccat
    75841 gccggaagca aaccaggatt ggaacttctc ctccgctccc tcaccggacc cgaccaaggc
    75901 gatcaattca ggacagcgtc gaagataggt cagtgccgtc tccgcgtcac gctcggcgac
    75961 cagcaacccg tagcggagca cctgagtccg ccattcctcc gaaggaagac ggcggagcat
    76021 cgcaggcgcc tccgccaata ccgcaatacc cgatgccggc gaacgctcgg ccatctgcgc
    76081 tccgatgccg atcacctgat tgcggtgtga cggcccgggg atatcttcca atatggccgg
    76141 actcgttctg aagaattcca agactcccaa gtagtccggc ttgccgagac tattctcgac
    76201 gatgagcttc atgccgaacc gcacccaggc acgaacggcg tcgagtgaca acacgcgtgc
    76261 aatcgagggg atctgccgga tatattccac cgccagggca aaatcggttt ccgtgagttc
    76321 acaggccaga tccatccaca cgggccaatc cgccggagac aagacgccga ccgcttccgg
    76381 tgcaatccgg ataaattcca ccgccacatt ggcctgacgg tctgcgagtt ctaacccggc
    76441 agccagcaca atcgctctgc tggagggctc gaggagaccg agcagcagcg gactctcctt
    76501 aaaaaatctc agggccaacg caccggaatc tgccgcgatg gccaccccga gatcgaccca
    76561 tggcagcacc tccgccagca ggccgcgacg ctgcatatcg gccaacgcat cgatcgcagc
    76621 cttggccgct ttcggcgccc cttccgtgag ttcgtccaac aacaccagaa caccctcaac
    76681 ggcatcgggc ctggcagccg agtcggcaat agccttcacc acggcagcag ccgtgtccgg
    76741 cccgagttgt tcggccagca ggtcacacag ttgttcagag gagcgcccag ccgccatagt
    76801 gccagaatcc gttttggggg aagttgtgga ttgtaaagga cacaatttcg cttgtaaact
    76861 gtgtcttcgc ggtgggcagg gaccgatggc gcacaggcaa cgattcaccg ggcagttgta
    76921 acgcggggga caggatggct gaaccgagca aagaaagcct cgacaagatg tggaagtatg
    76981 taaagggctt cgccgagaag agcgggaccg ccatgcaccc gaatgaagcc gtcaccaacg
    77041 cagtggtcct cggtctcgcc gcccacgtcg acgagttggg gaagccgtta tgcccctgca
    77101 acttctaccc cgacaagcag gccgaagcca agctgcggcg ctggatgtgc gcctgtgacg
    77161 aaatgcagat gtacaaatac tgccactgcc tgctcttcgt ccgtgaagac ggcatgccga
    77221 tcaccgaata tctgccggaa gaccacgaag gccgccagtg ttacgggctc atcaccgatc
    77281 cgactccgga gaaaggccgc gcgctccggc acaaggcact gccgatggcg ccgaaagaaa
    77341 ccactccacc gaccaccccg gaatccgccc cgcaaccgtg atcgagtcta tgcctcccgg
    77401 ccggaatcct gccgttgccc tcatgctctc cgccctgtct ctcctgctcg gcctggctac
    77461 taccggatgc ctccgtacga tggaggcgat cgacccgagg agcttgccga ttacctcgac
    77521 ctccggccaa cgcttgcagc accacgtgtc gtttctcgca agccccgagc tgtcaggacg
    77581 gcagccgggc tcacccggga atcgccaggc tgcccgctat atcgaggccc agtttcgagc
    77641 cgtcggactc gaacccctgc cgtcactcgg cggctaccgg caagccatct cgcccacgat
    77701 cggcgacaat ctcctcggct tcatcccgcc gaccgccgga tccgcccccg cgcaatggat
    77761 ggtgatcggc gcccactacg atcatcttgg ttatccattc ctgggcgcgg acgataatgc
    77821 ttcctcggtc gccatcctca ttgaaaccgc acgacacatg gcaggactcc cccagcacgg
    77881 catcctgttt gtggccttca acagcgagga atccccgtat ttcggtaagg ctgagatggg
    77941 gtcgcagttc ttgtttcacc acctgccgcc ggaaatcggc cgtgccgaga atctccaggc
    78001 cgtcgtgatc atggacctca tgggtggcgt acagtggccc cctatcaagg atgcgatttt
    78061 tgccgccggc gcggaaaaaa gtccggcgtt gtaccagcgc gtcaaacaag cctcgtcacc
    78121 atcgctgacg gtcttccctg tggggatgca cctcgtcgaa gagattcccg aaatcggcca
    78181 caaagccttc agcgactacg acgtcttccg caaccatgga ctgccgtttc tgtttctctc
    78241 cgccggacgc accccgcggt accacacggc cggagacgtc accagcaccc tgcattacga
    78301 ccgcatggcc gccaccgtcg aatggctgcg acacttgatt gccctgatcg gtcaggatga
    78361 ggcgccctac ggatttcagg ccgatcgtgt ggagtgggcc gatgaagtgg tgtcgttccg
    78421 agagatcgtc aaccgggcgg ttcaagacga gacgcggatt ccaggcacgt cgcgatggtc
    78481 gttgcgtaaa ttgaggcagg atgcggagtg gctgcgggat gccgatcgtt cagcgccgac
    78541 gccgcaggac cgtgatcgac tggagcggat ctcgatccgt gtgcaatgcc tgatggccga
    78601 ctactccggc tgtttcacct tttgatcgat ccggcctgtg cacgcgcaga acatcagatg
    78661 catcgttatt gccagccggt cgtctcatat cctttttctt tgagacaacg ttcaacgaag
    78721 gccgcatagt ccggctgcgg ttgcagtggt ttgtaggtcc cggcaaggaa acccaacacc
    78781 cccccgaccg cgccaccagc cgccgccccg atggcgatac ccgtcggccc gccgatcaac
    78841 cctgcggacg ctcccaccgc cgccccgccg acaatcccca acgtggcgcc ggcgccggcg
    78901 tttccgctcc gattggtccc gtgctgcaaa ccggcgcgtt ccgctttctc tccgcacacc
    78961 gccgcgtcac gtgccgcctg gtctttgcca tacagctgta cgcgagtgtt ggatttcagg
    79021 ataggctctg gcccggcaca ggctgtcaaa ccgatcacga ccaggagcat caggcaccat
    79081 gacgatcgcg acgccttcac atcctcacct cgctgcatgc cgaacaccgg gtttgtcccc
    79141 gccgaattga acctgtctgc cctgtcatag ccatccgatt acctcgcatg cttttccccg
    79201 ggcgacgcct ccggacagca aggctcgcga tctacccgcc actcatgtca ttccaggtgg
    79261 agttgtgcgc gcacctgttc taccgatcgc cccctgacca agacgcgttt ctggcgactt
    79321 cccgcgccgc tcaggatgtg aaccgcgctt aacggcacat cgcagcgacg ggcgagaaac
    79381 cggcacaatt cgtcgttcgc cgcgccgtca gccggcggcg cggcgatgcg aatcttcacc
    79441 gcgtgcccat gcagacccgc acattcactg cgggaggctt tcggctgaac atggactgaa
    79501 atggtcacgc cctcggatgt ggcgcgaagg gcctgttcgc gttcagtggc ctggcgctct
    79561 cgcatggggg tgcggctggt cgccgagctc ctgcagacac cgcctcaccg cgtcgcagtg
    79621 ggcggatgcc tcagaatatc gccttcacga cttcgatgat gctgcgcaac atgtccggat
    79681 catcggtaat aggacgggca atggcgacat cacaggcttg atcggccggt acgccctgcc
    79741 tgatcaatgt ggccgcataa atcagcagtc gggtgctcac gccctcttcc agtccgtgat
    79801 tcttgaggtt gcgaactttc tccgcaatct tgacgagacg ctgcgcaacc tcgaggctca
    79861 cgccggcttc atgcgccacc acccggctct cgacatcggg caaggggtag tcgaactcga
    79921 tcgccatgaa ccgttgcttg gtgctctgtt tgagatcttt cagaatactt tggtagccgg
    79981 ggttgtagga aatcacgagc atgaagtcat cgaccgcttc aatgacttgc cccttcttct
    80041 cgatcggcag caggcgccgg tcgtcactca agggatggat aatgacggtg gtgtctttcc
    80101 tggcttcgac gatctcgtcc aggtagacga tggccccctg cttgacgccg agggtcagcg
    80161 ggccgtccat ccagaccgtt tcctgcccct tcaacagata ccgaccgacg agatcggagg
    80221 ccgtgagatc ttcatggcaa gccacggtaa tgagcgggcg ccccaggcga taggccatgt
    80281 gctggacgaa gcgcgtcttc ccgcatcccg tcggcccctt cagcatcacc ggcatgcgac
    80341 tctgagccgc aatcgtgaac agcccgacct cgccccgcac ttcggcataa aacggctcgc
    80401 gcgcgatccg ataccgatcg atctcctgtt cctgcccttt cggcgtcgat gccgttcgcc
    80461 cttcgctcat tccgacactc ctacattgaa ttgaataacc tgcatggtgc gccacgataa
    80521 acggaaacga gcgggaagtt caagcagccg actgaagaca gggaaagccc cgcgctcttg
    80581 cgcggtacgg tcaggcgact ttcgatccat cgagcacttc tcgaaccttt tgcgccaggg
    80641 tcgtgggcgt gaagggcttc tgcaggaagg aatgctcggt atcgatccct cccttggaaa
    80701 aggccgggtg atccggatag cccgacatgt acagcacttt cacctccgga cgaaccgtcg
    80761 cgagtttttc ggcgacttcc ggtccgctca tctgcggcat gaccacgtcg gtgagcagca
    80821 aatggatcgg accagcatgt ttggcgccgg tgagcaacgc ttcaatcccg tgccgggcct
    80881 cgagcaccgt atatccatgc aagcgcagcg tttcattgac caaccgtcgg acacccggct
    80941 catcctccac caacaacacc gtctcgcgcc ctctgaccga gtcccctccg accgcgacgg
    81001 attcggtctc cgttgcggcc gactccacac gcggaaagta aatcttaaag gtcgccccgc
    81061 gtcccggctt gctctcgacc tcgatacagc cgccactttg cttgacgatg ccatagaccg
    81121 tcgaaagacc gagccccgtg cccttgccct tctctttggt cgtaaaaaac ggctcgaaga
    81181 cgtgcgactg cgtatcctcg tccatgccgt ggccggtatc gcgcacgatc aacgccacat
    81241 aggaaccggg ctcggcgccc atcggcggac ggttcctgcg ttgcccgaac tgcacattgc
    81301 tggtttccac cgtgaggcgg ccaccgttcg gcatggcatc gcgggcattc accgcaagat
    81361 tcatgatgac ctgctccacc tgaccgggat ccgccttcag ctgccccaac cccggatcca
    81421 gcaccgtgca gagctcgacg atgtcctcgc cgagcaaccg tcgcaacatg ccttccatat
    81481 tgtggatcac cgaattcaga tccagcactt tcggcgcaat gaattggcgg cgactgaacg
    81541 ccaacagctg tccggtgaga ccggaggcgc gatccgccgc cttcttcact tcctccagtt
    81601 ccttccggaa tccatccgtg ggcgccaggc ggctcaacac cagttcgctg tatccacgaa
    81661 tcaccgtcag catgttgttg aagtcgtgcg ccacgccgcc ggccagccgt ccgacggctt
    81721 ccattttttg cgactgctgg agctgcgctt ccgtgcgccg caacgcatcc tcggcccgcg
    81781 cgcgttcacc gaactggccg attttcagtc cgacatcggc caccatcttc aatagctgct
    81841 cgtccggctg cttgatctcg cgccggaaca actcgatcac accctccact tcggtgccga
    81901 cccgaacggg aaatccgaag gccccgtgta accccactct ggcagcggca ccaccgcgag
    81961 ggaaattggc ctcctgcacc acgtctttga tccacgttgg ttccccggcc tgccaaatcc
    82021 gtccggggag tcccttgccg atcgtaaagg tatgttgcca ggtgtccaac acaaactctt
    82081 cggcgttcaa tgcgggcgac tgccagacat ccagacaacg cagcacactt ccctgccgat
    82141 cgagccgcca gaatattccc aactcccatt cgagactttc gcagatggcc tgcaggatct
    82201 tcggccccgc ttcttccagc gtccgcgact ccgccaagac gcgcgtgacc gcgtactgag
    82261 acgcgagttg gcgctcggta cgtttccgag ccgtaatatc ccggatgaac gcgctgaaaa
    82321 tataggcatc gcccagccgg gcaggagaaa tggtgatttc gacgggaaac tcatgaccgt
    82381 cgcggtggga tcccatcatc tcaatccgtc gattgaggag tgcaccctgc ccggtatcca
    82441 ggaaatggcg cagtccgcgc tcatgcgcgt cccgatcgcg cacaggaatc accgtctcgg
    82501 acacgcggcg gccgagcgct tcctcccgct cccagccgaa aatattgacc gcctgcgcat
    82561 tccaatccgt aatgatgccg cccgcatcca tagaaatgac ggcgtccagc gccgtatcga
    82621 cgatgacccg gttgcgttcc tgactctgca gcaacgcggc ctccgccgca cgcgcccatt
    82681 cactttcctg ctgcgcctgc cggtgctgtt cacgcaatcg accggtggcc cagagcaaca
    82741 ggccgaccat cgggagccag acgacgacac cggccatgcc cagcttccac atcatggctc
    82801 gaatcggcag gagaatgagg tcacgatcca tccgcagcag gatcgtccac tgcaatccgg
    82861 agtagtcgct ataccccttg gtttgagaat aacccgtgac gaccgggacg tgacggcgaa
    82921 catgcatctc ttcgacgtag cccgccttgc cggaccggct caacagcacc gatggcaacc
    82981 cgagttctgt gagattcacc cgatcgatac cggaggaagc tgagtccaca aatacgacac
    83041 cggctttcgt caggaattga tactcgaagg ggccggcgga gccatcggtt tgttgaattt
    83101 cacggaccgt ttcaatgagg acatcctcca gcatcggcag cccgatgcgg gtcgtcacgg
    83161 caccgagaaa ctggccgccg acactcagaa tgggggccgt aaaggccacg gactcgatct
    83221 tatgattcga ttcatggatc tcgacttcat ccacctgcac ggttccggtc tgccgcaccc
    83281 ggtcgaacca gccgcttcga ctatagtcct ggccgatcac ggaggagtcc gtcgccgcca
    83341 ccatacgccc atgggcgtcc gtcacgccca gccagagata gaccggcgca taggtttcct
    83401 tcatccactg cagatattcg ttgatgtatt ttttgtcgag ggcgcgatcg gaaaaggctc
    83461 gagccatcat gcgaacatcc gcatgacgtt cgaacaacaa ccgatcgagt ttgtccgcga
    83521 tttcagcgga ggccagcgtc agattttctc cggtcgcttc gaccattcgc acttcaatgg
    83581 agcggagcat caccacgccg atcacaagcg ccacaatggt cacgccgacg atcaggaccg
    83641 gcaaccaggg ataggaccga cgcggggaac cggctggaag cgaggcggcc atactcagga
    83701 ctccgtcaca atgtagtttg gtaacaatcg tgcgggatga taccactgct tggaacgggc
    83761 cagactcggc aggccggaat catccatcgt gttgatccaa catgcccctt tcgcgcgagc
    83821 ttggcgacag aattcacgaa acacaaacgg cccgagcccc acaatatccc gatcggcgac
    83881 ttccagcaac acacaaaaca ccgacgggtt gagccacagg cccatcgtat aggcccgaat
    83941 acggcctcca acccgaacca ctgcgccgat cagcccgagt tcggcagcgt cagacaatgc
    84001 cgtctcatgc gcgccggcgg catcctccaa cagcgcacag gcccagtcat cagaaccggc
    84061 ctgctgcttc tgttcccgcc attcatgaaa caagctgaca caggctcttt cgtcccgcgg
    84121 ctcatagggc tcaagcatcc cgccccgttc cctgataaat cgattgcacg cggcccgcgg
    84181 ggatttaaac gactcgccgg acaagtcgga cagagctgac gcgtcgtaga gatagtcggg
    84241 ctctttcgca gtcgcacggt agcccagtgc gcgaacctca tcgacacagg cctctggcac
    84301 attctcgacc cgcgtgaccc gggaatcgcc gttccgctca cgcatgaaac gcaaggcctc
    84361 ggccaacggc tgcgcgagag gaccaggccc aagcgggggc agggccatga acaggccatc
    84421 gggacagtcg gcgaacaaac agagatggtc gtggagaaca gcccaggaat aggtcacgcg
    84481 atgtcgccag atgtaatggt aggcgaagga agcggctgcg agcggcgttc cctgaacacc
    84541 ggcgctgacg gcgagcgccg cgtccatgcg cccccgatcc ccgagcgtga aggcctgcgt
    84601 cctcacccgc attcgcccct gctgcagccg ttcggtcacg cgttcgagcg gccgcaacac
    84661 gatcacgtcc tcctggaatc gcccaatgag cctgggatac cgcacatgtt tatcgatcct
    84721 gggatcttgg tgtatcaatc cggatatttc gtcggcagcc tgagccacga gcggtgaaga
    84781 ctggtccctc atatacggac acttcgtgtc ccatcccaac agcacctgct catgcctggc
    84841 atcccacatc aaggcaaacg ggtagagtcg gcaatcgagg ggacggacct catagatgcg
    84901 acaccgcgag gttgcgcaat cgaaggccgg acagacgtac ccgtcccctt ccggattcgg
    84961 caccagatcg atttgtgagc catcggggtt tgggaacaac gcaggggcaa gcccggccgc
    85021 gattgcggtc ccgatttcgt cagcggtaaa gaagggccgc aggaaactgt cccgctccgg
    85081 gaaccggcaa cagacatcgc aactgaggca ggcggaactc ggcacgatct gcaggagagc
    85141 atcacccccg ggacgagaag gatttgtcgg gacaattggc acgaatgaac tcgtcatgag
    85201 ctgtgggcgc attgtagcac taacaagtgg agcgtcctac tattcgaaac gtttcacccc
    85261 cgcgttgccg cgctctcacg gggcggtaac gagtcgttct ttgaacagct ccggcgctcc
    85321 gctgacttgt accggtgtca gaggccatgg tacgatttgc tcacctcgaa tccaaacgcg
    85381 agtgcgaggc atgcgactgc gctgcgagtc tcgaggatat tgcattggca ctggcacaac
    85441 attcacgtga gccattgaac tccgacgaag cccgccgacg ttgtctcgca attcgtgatg
    85501 ttctcgttcg cgccggggtc tacggcccgg ccgaaaccgg cgacggcgcg gcaacgaccg
    85561 actcctcaat tccgacgcca cggttccgcc tggcgccaac gccatttccg ctctcctcct
    85621 cggacgtcac gttttttcag gagctgggac aggacctcct ctccttctat cgggctctga
    85681 accgtctgta tcaggacagc acccgcggaa ctcagcccgc ctgggtggcg gcctatctcg
    85741 accaaggcaa gccggaatcg ctgatcacct atagccggat gaaccgtttc cgcaccctca
    85801 ttcccggcat catccgaccc gacgtgattc caaccgccga tgggatggtg atcacagagc
    85861 tcgattcggt accggggggg atcggcctga ccggttgcct gactcgcgcc tatgatgatc
    85921 agttcaccat cgagtgccag ccccccggtc tgccgcctga gcgttctcct ctgatcgcgg
    85981 ggcgtgacgg gatgttacaa ggattcgccg ccatgctgcg cgcccagcaa ggcgaccagg
    86041 aagggtgcct cgcgattgtc gtttccgatg aggccaagga ataccgggca gagatggaat
    86101 ggatggccca acgattgcgt gagatcgggt accaggcctt ctgtgtcgaa ccacgggcgg
    86161 ttcatttctc ggaagagggc ctgtttcttc aggaagaaac cggaccacgg ccgatcggtt
    86221 tgctgtaccg ctttttcgaa ctgttcgact tgaagaacat ccccaaggct gaactcgtga
    86281 tgtatgccgc caagaagggg caggtgacgg tcacgccacc ctacaaaccc gcgcttgaag
    86341 aaaagctggc gttcggcctg tttcaccatc cggaactggc tccgttttgg gaacaggcat
    86401 tacgtcccga aaccgccatc aacttgcgac gattgctgcc gagaacctgg atcctcgacc
    86461 cacaaccggt gcctccttcc gcggtcatcc cgcacctcac gatcagcggt cgcgcggtgt
    86521 cggacttccg gcgcttagcc cagaccaccc aaaaggaacg ccagttagtg ctcaaaccat
    86581 caggattctc tgagctggcc tggggcagcc gtggcgtgtc catcgggcac gacctgccgc
    86641 aggtcgagtg ggccacggca ttggagcagg cgctccaggc attcccgacg acgccctatg
    86701 tccttcagga attccacaag ggccgcctgt tcgagttgtc ctatttcgac gagcggagcg
    86761 acacggtggt cccgatgtcc gggagagtcc gcctgtcacc ctattatttc gtcgtcggcg
    86821 ataaggccga actcggcggg attctcgcga ccctctgccc tgccaacaag aaaatcatcc
    86881 acggtatggt cgacgcgatc atggcgccct gtgccatcgc gccaaacctc gcgtcataag
    86941 gtgacctgat gatcgctgca gcagaaccag ttcatttgca gatatctcct caaccttttt
    87001 ccccataggt actcatggcc ctgacgctct atcatgtcca atggtgcccg gattgtgcgg
    87061 tcgttcggga ccgtctggac gaactgaaac tctcttacga agacgtggtg gttccggact
    87121 ttcgtccgat gcgcacacaa gtcttcgagg tgtcgggcca atactatgtc ccggtcctga
    87181 aagacggaga caccgttctg acggaaaccc acgacattct tgctcacctg cacacacagt
    87241 acgacaaggc acgcccgtga ggatctcgcc cggatctcca gcgcgagcgc cgatcacgga
    87301 ccgaccccga tccctcgggc aggcgccggc ctgcacggct cggccactta gtaaattttt
    87361 gacaaacagt cgtcgcccat gtgcttttca gagccggctg cgatcacgta tttccatacg
    87421 catcacctac aggccgttcc aaaaggccgc ccggcaaggc cgcagcgagc gaaacggcga
    87481 atcgtactcg tgccgtacgg tgagccgctg agcgatgcga gaacgccgct ggcggacttt
    87541 ttcaacagcc tgttagaaag gatagagacg tggaggactg gagacgcatc ctggctcaga
    87601 gcgtggtcaa gccgaaggat ttggcggatc ggctcggtgt cgatccgaag gaaatcgagg
    87661 acatcgtggg ggactacccc atgcggatta cgccgaccgt gctggccacc atcaaggaaa
    87721 agggcgatgc gatctggaaa caagtcgtgc ccgatcgcgc ggagatggcc gatgcggatg
    87781 cggaagacga tccgctcgaa gaagatctga tgagccccgt gccccacctg gttcaccggt
    87841 accccgaccg cgtcctcttg atggtcacaa atcagtgtcc gatttattgc cggttttgca
    87901 cgcgcaaacg gctggtgggc aaaccgggct tcctcaagaa aggggagctg gaccgggcga
    87961 tcgcctatct gcgcgaccat caggaagtgc gggatgtgat tttgtcaggg ggcgacccgc
    88021 tcctgctgcc cgatcacctg ctggaacgga ttctcaaatc cctccggacc attccccact
    88081 tggaactgat ccggatcggc acacgcgtac cgggcagcct gccggagcgc atcacaccga
    88141 agctctgcga gatcatcaag aaatatcatc cattctatat gaacctgcac ttcaaccacc
    88201 cggacgaact gaccccggag gtgaaacgcg cctgcgggat gctggccgat gccggggttc
    88261 ccctcggtgc tcagactgtg ttactgaagg gggtcaacga cgaccccgag atcatgaaac
    88321 gtctgatgca tcaattgcta ctggcacgcg tgaagcccta ttacctgtat caagccgacc
    88381 tgacgaaggg gacgaatcat ttccggacgt cggtggaaac cggcctcaag atcatcaagt
    88441 ccctgcaggg ccacaccagc ggcatgggcg tcccccactt tgtaatcgat gctcccggcg
    88501 gcggaggcaa aattccactg ttgcccgcgg actacctggt caacctggac gaagacagcg
    88561 ccgtgttgag aaattacgaa aacaggacct tccactaccc ccagcccagc tccactccag
    88621 gacgtgagct gccgatggtc ggcgcccatc cctcctgggc ctccacagga aacggctgcg
    88681 acggaggggg tgaacacctg tgacccgtgc cgcttcttca aaagggattc tgccgtatca
    88741 gcacatcacg cagctgattg cccgtgggtc catcacatcg gaccatccga tcgaagaccg
    88801 gcaaatccag ccggccagtc tggacttgcg gctcggaaag aaagcgtacc ggctgatcag
    88861 cagcttcctg ccggaattgt cggctatcag cagccgcctc aatgtgctcg acttttatca
    88921 gtccgacctc gtgatgtatg agatggacct cacgcagggt gccatcctcg agaagggaca
    88981 tgtgtatctg gttccgctcc tcgaacaact ggacctgccg gccacgctcc gggcacgcgc
    89041 caatcccaaa agcaccaccg gacggctgga tgtcttcacc cgcgtcgtca ccgacctgaa
    89101 tgccggcttc gatgaaatcc gagccggcta ccggggcccg ctctatctgg aagtggtgcc
    89161 acgctccttc gccatcaagg tgcgcacggg tgactccctg aatcagattc gtttcgtgcg
    89221 cggcgatgcc acggtctccg acagcgccct gcagaagctg catgggaccg atccgctgct
    89281 ctttcgtaat gcctcacccc cgaagccgct gccgcagaag gagttccgta cggagcgcgg
    89341 cctcttcttg cgaatcgacc tcaccggaag tgcgcgggaa gatgcggtca tcggctaccg
    89401 ggccaagaag aacagccatg tcatcgatct ggccaaaatc ggacattatg cggccctgga
    89461 cttctgggaa ccgctgaaac ggcaccggca ggacagcctc ctcctggaac cggaggagtt
    89521 ttatatcctg gcgtcgaaag aacgcattcg ggtcccgcct gggtatgccg ccgaaatggt
    89581 cgcctacgaa gcggcctgcg gcgaactgcg cacccactat gccggcttct tcgacccggg
    89641 cttcgggtac ggagacggca agatgcgggg cactcaggtc gtcctggaag tccgcccaca
    89701 cgatgtgccg ttcctcattc atgacgggca aaccttcttc aaagtagtgt atgatcgaat
    89761 gctcacggtg ccgacacagg tatatggaac ggcactcggc tcgtcatatc aacgacaagc
    89821 cctcaccctc agcaagcatt ttaaggccta gacatggcac cacccagcga ccttccagat
    89881 ccttcgaccc gtccgtccag gcccccgttg caccgcccgg acgacgaaag caatgtagac
    89941 cgccagacgc atgtcgtcgg gatgatggtc ggcacggcct tgatgttcat cggctttttg
    90001 gacgtgttcc tctctatcag cggtggattc gagatcaacg tcgtgcccct gctgatctac
    90061 ttcagcggca tcgcggtctg ggccaatgcc gtggtcgaaa atccaacgat ccggtactcc
    90121 ttgatggcgg gatcggttct gatcagcctg gcattttttc actatggcga agtgctgttc
    90181 tggcataagc aggtggtgtt ctggtccacc gtcgtcgtgg tcatgtattt catgttcaac
    90241 gagccgaaac aggcaacctg aagactccgt gccctgccca tcttgagatc gggccggtga
    90301 attcatcgct cttcctcccg caccgatgac cattgccgtc atcatcccgg ttctcaacga
    90361 agccttcggt atcgggcaga ccctctcaca cacggctacg ctcggattcg acgagctcat
    90421 catcgtggac ggcggcagtt cggatcagac ctgcgccgtt gtcgagtcgt gtgccggccg
    90481 ccttcagaac aggccttact caggccccgc accagcctcg ccggctatcc gtcttctgaa
    90541 ggcggccgcg gggcgcgcgc gccagctcaa cgccggtgca gccggcagcc gctgcgaggt
    90601 gctcctgttc cttcacgccg atacgtacct ccccaccaac gcccggcagg ccatctccac
    90661 cgcgctgtcc gacgaggctt gcgtcggcgg acgcttcgac gtccgcttcg actcccctcg
    90721 tccgatagcc cgactcgtcg gacacatgat gaatctgcgc tcccgctgga gcggcattgc
    90781 cacaggcgac caggccctct tcgtccgacg ggatgtcttc gagcggaccg gacggtttgc
    90841 ggagattccg ctgatggaag acatcgagtt ctcccgtcgc ctcaaacgag cagggcgcct
    90901 tgccccattg cgtaatcagg tcgtcaccgc cttccgtcga tgggaacgga acggccccgt
    90961 ccgcacaatt ctgttgatgt ggaccctccg cttcctgtac tggatcggag tcagccccta
    91021 tcgactccaa catttttata gcattgtgag ataaacccat gagtgctccc ccgtcggacc
    91081 gcaaacagcc cgcatcggcc gccctggtga tcttcgccaa agccccgatc ccgggacagg
    91141 tcaaaacacg tctctgcccg ggcctgaccg aagacgaagc cgccacactg cacggcagct
    91201 tcgtcctcga caccctcgaa cgcacgaagg cggcggtgca taagttcaag cttcctgtcg
    91261 atcgctttct ggcctgtgcg ccctcaagca cccatgtctt tttcagaatc atggaagcgc
    91321 gccacggcgt cacgctcctc gatcaaccag gcgacaacct gggggacagg atggcgcagg
    91381 cgttcagcac gctctttgcc cgtgggtacc ggcacgtttt cctggtggga accgacgtac
    91441 cctctctgcc cctgacacat tatcgcgatg ccgtgcagtc actcacccgg cacgatctga
    91501 tcctcggtcc ggcaaaagac ggcggctact atctgatcgg catgacggcg ccacaccccg
    91561 acctgttctc ggatattccc tggtcgacgg accaggtcat gggactcacg cagcagaaag
    91621 ctgcggccgc gggattgaag accgccctcc tgccggcctg gagcgacgtg gacaccctcg
    91681 acgatcttca cgccttgatc gatgattgcg cagcggacaa aaaacgcccc aaacaggaac
    91741 gagtgtattc gcaacgaacg gccggtacgc tggagttgct cgcgaaacgc ctgcgcacgc
    91801 gccacacgta acggagtccc tatgccgaat aaagttgccc tcatcaccgg cggcgccaag
    91861 ggaatcggcc gcgccatcgc gctcgatctc gccgcgcaac aatggtccgt ggcgatctgc
    91921 taccgcacca gcaaagcagc cgcggatgaa accagcgcgg ccatcagggc acgcggcggg
    91981 caagccctga ccgtgcagtg cgacgtctca gacccgaagg ccgccggcga cttggtctca
    92041 caggtcgaaa gccggtgggg aagaatcgac gcactgatca acggcgcagg cccctatcac
    92101 cgcgtcaatc tcttcgacga gacgccggcg ggctggcgtg aaatgttcga gggaaactta
    92161 cacccgattt tttatcttgg gcaggcggtc gcacccggga tgaaggcacg gaagcaggga
    92221 cgcatcatca acttcagcat ggccaacgcg gaccagatgg tagcccagcc ggaggtgacg
    92281 gggcactata tcgccaaagc cggcgtgtta attctcacca ggacactggc aaaactgctc
    92341 gccccctacg gcattaccgt caacgccatc tcccccggat tcatcgattc gggcagcgcg
    92401 ccgcccgatg aactggccgg tgtggtcaaa cgaattcccg ccggatacat cgggagcgtg
    92461 ggggatacgg tcgcagccgt gcgatacctg ctcagcgagg aggcgcgcta cgtcaacggc
    92521 gccaacctgc acctcagcgg cggatggggt atctgactcg acggtttacc tggcaaagaa
    92581 aagaatggct ccgatgatcg ccgccatgag ataacttttt gaatccttga ccgcgaacac
    92641 caccggatcg tcgtccattc gattccggta ggccagcatc caaactcgac tgatccagta
    92701 gagcatcacc ggacagacca gccacaatac gtccgcatgc gtgtacagca gcagcacttc
    92761 tttactgctg atgtagagcg ccagcaccaa cacggccagc aatccgctcg ccgtgccgat
    92821 agtcgacaca tgttccagat ccgtcaccca ataccctcgc ccgtgcagtt ccttgccttc
    92881 ggcctcaccg gtgagccgca gttcggtaaa ccgcttcacc aaagcaagac tcaggaagag
    92941 aaacaacgag aacgtcagca gccaatggga gggagccacc cccgtcgcag ccccgcctcc
    93001 gtatacccgc ac
//
LOCUS       DAORRQ010000008        88625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_8_length_88625_cov_55.006052, whole genome shotgun sequence.
ACCESSION   DAORRQ010000008 DAORRQ010000000
VERSION     DAORRQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 88625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 88625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88625
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8_length_88625_cov_55.006052"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PK224_04110"
     CDS             complement(<1..668)
                     /locus_tag="PK224_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14952.1"
                     /translation="MMPSIDKSPETATLELVERQRKLYEGSEIDVRLEHLIRDTPHPT
                     LSSWDIQRLLHDSRAVYFDSHVEVVSGHHTGVYLRFESIARFPDLVTIIASNMAEWIL
                     QTFRNHPPTGILTTSSDARFLAERTSDILAPHMPLRVVLTPFNHETGKIGTDIIQGTV
                     ETGERFLSLNDVTTRGLCVSKLGKVVTDHGGQLAGMMVFARRDSGQFPLMDELTARFP
                     FYFSV"
     gene            complement(682..1665)
                     /locus_tag="PK224_04115"
     CDS             complement(682..1665)
                     /locus_tag="PK224_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRB14953.1"
                     /translation="MKLVSFQVRTPVGTFTRIGALHNASIVDLNMAQARRLTDQGETQ
                     PRRLADAQVPATMLEFLEGGPAATDAARRAFDYAVGHGSSIAGPAGETIFYDPASVRL
                     TAPLPNPSSLRDFIAFEEHIAATSKRRGQPIPPEWYKAPVYYKGNHRTIIGPDHELRW
                     PLNTQKLDYELELACVIGRKGIDIAERDAVNYIAGYTIMNDFSARDIQFQEMACRLGP
                     AKGKDFATAIGPCIVTPDDIQDLTSLRMIARINGEVWSEGRFGSIHWSFSQMIEHVSR
                     GEFLYPGDLFGSGTVGGGCGLELDRYLKPGDVVELEILPIGILRTTIAAQN"
     gene            complement(1662..2777)
                     /locus_tag="PK224_04120"
     CDS             complement(1662..2777)
                     /locus_tag="PK224_04120"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRB14954.1"
                     /translation="MYLVKKGAPPNQAHVGVPDGLHEEEHGRQGFSGPSSHLYHHHPP
                     TAWSRIDGPLRPRAFTCTELPTPDCHSADGRPTLVLQSPDARVSLSRRAETMPHFVRN
                     ADGDEIVFVHRGRGRWETDYGFLSYEPGDYLVIPKGTAYRVHVDTKEEPGLFLMVETT
                     APVEVPDRGPLGRHALFDPGVLTAPELALPPEDAHSRQEWEVRIKRDGDYTRVYYPFY
                     PMDVAAWKGDLWVAKLNVRDFRPVISPRYHLPPSVHMTFQAGGLLISTFAPRPLESDP
                     SALRVPFYHRNMDYDEVLFYHQGDFFSRAGIHPGMFTLHPQGIHHGPHPQAIAASKTK
                     EQTDEVAVMIESRRAFTVMPECDAVELKDYAMSWSRP"
     gene            complement(2796..3395)
                     /locus_tag="PK224_04125"
     CDS             complement(2796..3395)
                     /locus_tag="PK224_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB14955.1"
                     /translation="MKEAIGIDHITLCVKNLAAVESLFTTVLGFHVIWSAQDVGSDMS
                     SMDTVVVQRGDAKIALMQGRNKARRSQICDFIDKYGEGVQHIALEVDDIEAVCREWES
                     HGVRFSGETKNGRDGFGPLKQRFTYPLFPGCGLFLELTQRQHGLEESKTSRRSIETLR
                     EIKPEGSRKQSSTTRPFRFPLKTRHPSRFSRRRKTSSRP"
     gene            3621..4388
                     /locus_tag="PK224_04130"
     CDS             3621..4388
                     /locus_tag="PK224_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HRB14956.1"
                     /translation="MSFYEHVRADVLRHGAINNTYLTRFQAGEVTDSEFHEFAVEFYS
                     FARFFPRILAAQLVNTEDEAVADELTKVLYSELGDGIVKHRHELLYRNFLRSIGIDIH
                     EAMHTPMKPSTRAYIQGMERLYSSRHHVTALGASFGLENMAITMWDHLIPGLTRLKQV
                     RYPRMDMTYFTFHRKLESAHEKAMEHAVEAVGGAGANGCATMSAQEQTDFCLGMNAVL
                     DYLEGFWMGLERKASGIRRSRPQPARQLASELRGQSH"
     gene            4413..4907
                     /locus_tag="PK224_04135"
     CDS             4413..4907
                     /locus_tag="PK224_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRB14957.1"
                     /translation="MERVWISRYDAGVPLSSAYPEWTVPDLLRRSASCFPESTALFFY
                     GARLSFRELNDLTTRFAHGLLRLGVKRGDRVALMLPNIPQTVIAYYGVLKAGAIVAPM
                     NPLYVEREIHSQLADAGSEIIVALDLFYPRIQAVREQTGLPERIIITSLGDSSDREAA
                     ALSA"
     gene            4882..>5598
                     /locus_tag="PK224_04140"
     CDS             4882..>5598
                     /locus_tag="PK224_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRB14958.1"
                     /translation="MKRLLYPLKARLAKRWVAVEKTPPVYDFLALLDAASPRTEDDSP
                     LLPTVEPDALAQIQYTGGTTGTPKGVMLSHRNVVVNAMQGRLWCSDFQEGKEVFLGAV
                     PFFHCYGLSTCQNLAVATGSQIVLLPRFHAEEAVKLIHQHRVTIVSGVPVMFAMITDC
                     PKVGRYDLHSIRVCLCGASPLPTEVQERFERLSGVKISEGYGLTEAGPTTHCNPIHGD
                     HPQGSMGLPFPDTDARIVDEE"
     assembly_gap    5599..5665
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5666..6132
                     /locus_tag="PK224_04145"
     CDS             <5666..6132
                     /locus_tag="PK224_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRB14959.1"
                     /translation="MQGYWRKTEDTQAVLRDGWLYTGDIVTRDEHGFFFFLDRKKDVI
                     KPWGETVYPREVEEILYQHPAVREAVVVGAPDHHYGEAVKAFVVQKEGCAVTERELIE
                     HCRRSLARFKVPVAIEFRTELPRTIIGKVLRRALRDEADPVPAGMQSSRKAM"
     gene            6151..7341
                     /locus_tag="PK224_04150"
     CDS             6151..7341
                     /locus_tag="PK224_04150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRB14960.1"
                     /translation="MREVVIVAGVRTPIGNFGGALKDLPPHKMGELVVREAVSRAKVD
                     PRLIDEVIVGSVGHTSDAYNVARAIALMAGLPVRTPAYSVQRNCSSGLQPFVNAYQNI
                     QSEDADAQVVGGVESMSRAPFVSRDMRWGKRLRNAELIDSIWEGLTDAFCGQLMGRTA
                     ENLAEEFGIGREEQDRYAVESHRRAFKAIREGRLKDEILPLMVPKSVAGRDVAPVVVS
                     QDEGPNVGLTAQQLALYPPLFKEQGSVTAGNSCPLNDGAAAAIVMSSARARELGCRPL
                     GRIRGYAFIGVEPTRMGIGPAEALPMALKRAGVTLTDLELIEVNEAFAVQYLAVERVL
                     GLKRELVNVNGGAIALGHPVGMTGARLVITILYEMQRRGAALGAVALCVGGGQGAAMV
                     LERM"
     gene            7373..8596
                     /locus_tag="PK224_04155"
     CDS             7373..8596
                     /locus_tag="PK224_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRB14961.1"
                     /translation="MYIYKVGVVGAGTMGAQIAEVVSYAGVPVLLADIQESLARRGVE
                     SVRAIYQARVDKGKMTPEQLEEKMLLVTASPNLEALHDVDLVIEAVSEEVTLKQRVFR
                     ELDRVCARSAILASNTSALSISAIGASTTRPGKVLGLHFFNPAYAMPLVEVIPGLATD
                     PQTVDDVVGFAESLRKTPVIVKECAGFLVNRLLSPYLNEAVWCFQDGDVSIKEIDQDM
                     VAFGMPVGPFMLLDTVGLDIAFEVARILHRSYGPRMAPAPLLEALVKAGRWGIKSGRG
                     FYEYSGGEERSGDQDLDTLLQQVRQGGGQTRMEWTRLRPLLAMVNEAVTAYQEGVASA
                     RDIDLAMVAGTGFPNEKGGPLHVADQLGIDHVLHELEALRDTLGVRFWPAPMLRRMVD
                     AGFTGQLAGRGFFAY"
     gene            8689..9477
                     /locus_tag="PK224_04160"
     CDS             8689..9477
                     /locus_tag="PK224_04160"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRB14962.1"
                     /translation="MTTTAGSMLSCTVEGAVATLLINHPPANTLTPELLAELSTTFDQ
                     VAKDEAVKVVVLTGTGRFFIAGADIRVLASIASSKEGEAMALQGQAILDRIEEVGKPV
                     IAAINGICLGGGLELAMCCHIRLAAEGSRLGQPEINLGIMPGFGGTQRLARIIGQSKA
                     MELILTGEPISAQEAKTIGLVSQVVSPEDLLRQAQGLARTIASKGQMAVRASLRAIRQ
                     GVELNLHDALALEARLFGELCDTADRREGLSAFLEKRQPHFMDR"
     gene            9507..10727
                     /locus_tag="PK224_04165"
     CDS             9507..10727
                     /locus_tag="PK224_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB14963.1"
                     /translation="MAPTFSKRALALGGGGFTGYLFEIGALTALDDLFEDGVTMNDLD
                     LYVGVSAGAAAASLLANGVRPQEILDTNLSGTRPYYFDHRNEFSPAIGEGLKTVWRVT
                     RQLVPLLKLYVRHYGEMTFIDLLDKAQDALPSGIYTLEPFAKYLEAIFKVKGLSDRFA
                     GLAKELYIPAIDLETGDGVLFGDEDWRDVPISRAVTASSAVPIYFCPVRIQGRDYIDA
                     GIGRMAFFEIAVQKHVDFMIMINPMARAPQTRSRQAQPAVGKPGNRLRDRGFLSIGEQ
                     ASRINFDARFSQALERFQQDYPEKEVLAISPVEADALLFERSFLSYDDRIHLLRAGYV
                     AVMTLARDRWEDLSAQFARHGLVMSRARFEDRAGRRLARLDEVARVADPNTLQPMVGP
                     ARIGGIGAAWRKAK"
     gene            10748..12685
                     /locus_tag="PK224_04170"
     CDS             10748..12685
                     /locus_tag="PK224_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRB14964.1"
                     /translation="MTNGAEMFKGSLAAAEAARDTRAYSGLIAGLFEGVVRRSLFTSA
                     SIPAMSGDAKEFLDTLRLLLIEKVDPEAIDREGAIGEEVIEAFRAIGAFGIKIPRSYG
                     GLGLSQSEYHTVATLLGSHDAATSVLLSAHNSIGVAEPVKLVGNREQQQRLLPRLARG
                     DISGFALTEKSAGCDIWDLKTYAIPVHEGGVLVGYRLTGEKLYTTNAPREDHEFLASL
                     LVVIAQIVTVPQDVDRPKEERRFGAFVVETQSAGCRCTRLSFMGVRGIYNGQVHLRDV
                     FVPVADRLGEEGEGLRRALESLTVGRLTLPAACLGSLKQCLWLARARAQQRIQYDRPI
                     GEHTDIGSKIVLMASRVLALEAIVKITGIWTDAKVDVRLESAAAKVLATEWLLESLLD
                     LFRIYGGRAFETPDSLRLHGDLPVPIERMIRDALINVIWEGSNGILTLWIAREGLGEY
                     VTQGRAFLERGIEDMARAMPFFARISARAFNPIAFLGRGVRSGADAAPWERFVSQQSR
                     ALARKALWVSARDRHNLPRNQRVLRSLVTAGLQLFAVEALLWYGSQPEVREHALSRAL
                     VEDFCLRVKETFHPTPLLSLTTPLWDDDRRLFHLAKDILAGKAGWLEEGIIPSCPGGR
                     TGVAEPAGVVTGASVETWSGI"
     gene            12740..13723
                     /locus_tag="PK224_04175"
     CDS             12740..13723
                     /locus_tag="PK224_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14965.1"
                     /translation="MTEISGYFEAIKDRYALTSDYTLAEKLGIAQPDANLMRRGLKVP
                     KPELCIKIAKLLDRNPVELLLIAQKDKAPKQAKEYWTLALTAVDVMLHVPKRPRYLPK
                     KVEAIGRELKQLESQTLTYEGAAANAEAVRLMETAEQSVDAMMERWNIWKKGEALYPN
                     YLLANQAAARRQVKIRRLLILTQAQMQDSMTVSDAVNVMDDQQRAGVKVFYAFREALV
                     QSPTFQRLEEDFRKHGAAEDMNTAMFDREILIFSQTYGTVPLGMVGTPTPITMINRLQ
                     ISWKPEMIRDLDPAPLFDMTRYVFEYEGVGSFREQLARFTRSMRELPRRAV"
     gene            14488..16305
                     /locus_tag="PK224_04180"
     CDS             14488..16305
                     /locus_tag="PK224_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HRB14966.1"
                     /translation="MACVKKSVGQDAGVNLSAGAGGAVSGGSSHPRKHWQLLRRWRRT
                     FASVTALLLLGVLALPAYSQTGSQLGPLPPKLPQLPVVPPPAQPPAIEVPAPRPPSAL
                     DQAAKGPKIAVKEIRLIGNTAFTLQQLSEVTSPYTNRELTAEDLESLRLALTYYYVNH
                     GYLTSGAVVPEQDVADGVLTMQIIEGKITQVNVEDTKWFRPSYFQSRVNLAAGPPLHV
                     EALQERLRVMQANPRIERINAELLPGATLGENTLNVKVKEANPLKAWLEFNNYQSPVV
                     GAEQGFITLAHQNLLGFGDTLSLQYGRSAGVNPMLNFKYEIPVGPRDTTVALQYRRFD
                     FGVEEAPFDALDIKNKAQIFGLSVRHPVIRTADQELAFSLTGEHARNESTLGGNPFEL
                     ITGSPNGKFRVTSLRFGQEYARRSADQVISLLSRFSVGVGAMGATANGDPNLPDARFF
                     SWLGEAQWIRQLPLWRTQLVSRGVVQLSNDHLFPLEQIAVGGRYSVRGYREFTLIRDN
                     AAMLSIEARVPVYTTKAGVDSVFLAPFFDLGHGWQTTAQTPGTPPKTLASLGAGVIWN
                     FWRGSHFELYYGKQLKRYDTDRNNLQDHGIHLQLVVEAF"
     gene            16442..17719
                     /locus_tag="PK224_04185"
     CDS             16442..17719
                     /locus_tag="PK224_04185"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HRB14967.1"
                     /translation="MTCRPAGLPLFIHGLLAFLMLLGHTGSIAYGQATNIVATPSGPG
                     GLGTSLSTSGNTTNITGGTRPGSGPNLFHSFNQFSVGTGDVAAFVNPGGVSNVISRVI
                     GGSPSNINGTVQALNANLFFINPSGIVFGPSASLNVSGSAYFSTAQQLRLSDGGIFTA
                     NTGLLAFDSTLSVGSPVSFGFFGQGPYGSIVLSSASTVLQTGAVLGLMGGGIQINGSK
                     ISAQRVILGSTSSAGEMSVQPFVGNPFSGASGNGQVQALPGTLIVAGGGGVIPASIDV
                     TPNVSVPTGAQVNTTTNPLNQRVTQIQVVGVNLGGLPPLPTANAASVNTANPVEPVAF
                     LNRAVVLLPQDAPAPPAPLLTSRCAARKDGAFSSLVQASRDVTPSQPGGSLAAPVVLE
                     EVGSEGEPGAPATQTAQRLGQSTAQLMESWQGC"
     gene            17738..20212
                     /locus_tag="PK224_04190"
     CDS             17738..20212
                     /locus_tag="PK224_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRB14968.1"
                     /translation="MPTKRNMVTRRGMKRRHGSAGPSATRALSSPLTMLGVGVLALSG
                     LAWPVSGIAMELFGGSGASSISPSQEMRQGTTQFQQGAFAQAAAHWMNAARGYEESGQ
                     AKEQCQALINLAHALQQEGQIRRAQGTLQAALKLSEQNGERGLTATILAQLGNTFHVL
                     GKDEPATEHLTRALTLAREENKPLLVAGVLNDLGNALTARRQFAEAIDVYAESRSLAI
                     ETKQPALAATAQINGAMALLQNQQLSEAHRHLDQAWSDVRSLGDGQAKTAGLLNIGLG
                     YQELFAATTAKSGVARKSDAGKGRATEAVASAGLGAGLLRQSSDAFTAAGEVAGRTGD
                     ARGQSYAWGYLGGLLEQEHRNPEALEYSRKATFAAQKVNAPESLYRWQWQTARLLRAA
                     GKEEEALAAYQRAVTLLKPIHYEYSVGYQGRHHSYYESVAPLFVEYEDVLLRRAAAAK
                     TPDQNEQLLVQVKDTVEVSHAAELQDYFQDDCVTTVASHRGVGTLAPGTAVVYPISFP
                     DRLELLLETANGLKQVRVPVAGEKLTKEIRSFRRLIQDSQSQNYLSSAQTLHGWLVAP
                     LQQDLQGAGIHTLVMVADGSLRTIPMGALHDGRHFLVDSLAVAVTPSLALTDLSAAQR
                     RKGSLLSVGLTESVEGLSAPRYAESEVQAIRTLYGGKLLMNKQFSAPSLEEEIKDQGV
                     GIVHVASHTVVGTEARDSFVLAHDGKITMDRLSQLVGLQQYRQQPLDLLTLSSCEIAA
                     EDDRAALGLSGVAVKTGARTALASLWTSDDETTTELVSEFYRQLQDPALSKAVALQRA
                     QQKILSQRGHTHPSFWAAFLLINNWM"
     gene            complement(20354..20773)
                     /locus_tag="PK224_04195"
     CDS             complement(20354..20773)
                     /locus_tag="PK224_04195"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14969.1"
                     /translation="MSSIQRAFRFAVERLAPSVRPTGTLSLYARFSGPLNAEGATAEA
                     ALAIGFLALLKGDHLKQDITITGTLEGDGRIGQVAQVAEKTTAAARAGYHVLLVPRGQ
                     FYAPRVNRVSLNLESNVLVREVETIEEAYEIMTGKKL"
     gene            21306..22169
                     /locus_tag="PK224_04200"
     CDS             21306..22169
                     /locus_tag="PK224_04200"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB14970.1"
                     /translation="MAVQGPIVITGASSGIGAACAQYLDALGFTVWAGVRSTQDGEAL
                     TRLTSSRLRVLMLDVTDPASIEAARKTLAEATRDTGLAGLVNNAGISVAGPLELLPLS
                     EVRTQFEVNVIGVLAVTQALLPLLRLARGRIVNISSIAGLAATPFLGAYCSSKFALEA
                     MSDALRLELAPWGISVSLVEPGAIQSQIWQRATMSATRTLGGVEPEALALYAQPLSRM
                     QEVMAAAAARAIPAEVVARVVADALIAARPRARYLVGKDARFRATLKWILPDRAQDRL
                     LAWFLGLPHRG"
     gene            22220..22642
                     /locus_tag="PK224_04205"
     CDS             22220..22642
                     /locus_tag="PK224_04205"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRB14971.1"
                     /translation="MPEAGLVRSAGGVVFRQQDVLLIRVSDIKGRPVWSFPKGRLDAG
                     ETPAQAALREVLEETGWCCRIEADLSTTEYWFQREGRRFRKTVVWFKMSALEEAGVPD
                     GEVEEVQWVDREVALGRLTYPSDVALLSQALSQGPSSR"
     gene            22906..26061
                     /locus_tag="PK224_04210"
     CDS             22906..26061
                     /locus_tag="PK224_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB14972.1"
                     /translation="MYSLARFSLLEMTECSAVLRQLGEQSSSLQDAASRAVDYLFRTL
                     GNPDTGARDCVLVRCFKTVSYNRLNPMTQQRVREQLGGIAPFPDLKCFTLMATAGEEA
                     EWNQVERSHRYRAIPMVSADFVKQFPMFSQLLHQFGLSSSFDVAPEGEWLVDLEEKTY
                     NVFYVPDAVGSPYVPVQEGFVLKYGVKSVLCFGGMLPSRDLFAVILFSRTPVPRDTAA
                     LCKSLALSLKTSLLACDETVPLEAGVDAHSSSAPAIGAPEQGAGLRHQSRLAVAEQLL
                     RVYENAVRTQSAGIAQAKQALWERANALERSEQALSEQTRIVNSVLRSMGDGVVVTDA
                     EGQLVVANPAVESILGFSPFDIPLTAWAERYEFFHADTVTPFGQDEWPLARALRGEKI
                     DWLEVYLRASQGMPGRWISINARPIKDEAGVLSGTVVVFHDITWLKRAQDALFESEYR
                     FRSLVEGARDIIFTLSADITITSLNPAFETVTNWSRSQWMGEPLVALLHPDDAGFCQD
                     VLQAILERGAPLTCSMQISTKQGGYVTGEFVGTPQMRQGRVIGVLGIIRDVTERRRIE
                     DALRVSEERLRSIVQSTKDAIVLVNALMKVAFWNKGAEATFGYSAEEIIGQPVTMIIP
                     EQYHEELERNVQRVRLFERVQLTSRTLELLGRRKDGGEFPLELSVTSWKGKSDLFFTI
                     IMRDISERRSAEEELDRLHHHNQVVLNSAGEGIYGIDRDGRLTFVNPAAAKMFGWEAE
                     ALIGQPFSTLVHRPDFREGASGERLSPIVETIQGGKIREEADSRFWRRDGTSFPVEYV
                     STPIRERGDIVGAVVVFKDTTDRKRAEEQLQDSLRRLRKLSGRMEGIREEERGRIARE
                     LHDELGVGLTCLKIDLSRLGGLLGERLEPRDRAKVDEKIRGMKEQVDSTITSVQRIVA
                     ELRPGVLDDLGLVAAIEWQCRDFQRRTGVACHCTVSHEDLRVDPEHATAVFRICQEAL
                     TNVTRHAQATEVHVRLEDQGGGLLLQVSDNGRGIPSDRLADARSFGLLGMRERAGLLG
                     GDVQIETKEGSGTTIALQLPR"
     gene            26093..26740
                     /locus_tag="PK224_04215"
     CDS             26093..26740
                     /locus_tag="PK224_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB14973.1"
                     /translation="MRTTGTVRILVVDDHPSFRRGVKDILEEGFEGASMAECGNAQEM
                     LDHVREQPYDLVVMDISMPGRSGPEVLKELKQLAPTLPVLILSMHPEDQYAIRMFKAG
                     AAGYLTKASAPEELVHAAKKVMSGGQYVSASVGEALALTVRTGVDKLAHERLSDREYE
                     VLCLIASGQTVSDIAESIHLSVTTISTYRARILDKMNLKNNAELTRYALQHGLVV"
     gene            27134..27523
                     /locus_tag="PK224_04220"
     CDS             27134..27523
                     /locus_tag="PK224_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14974.1"
                     /translation="MEQPTGSSGQRSGKRPTVAVVYEDVTTGYKCADWFALHGYQATM
                     TSAVHHLERNLKELRPDVIVVRLSSAPTPLHSTVPRLKMTCPQVPIIAMVDQSTDALD
                     GVAPDLTKAFGADVFMCDSLDPPSPPS"
     gene            27626..29038
                     /locus_tag="PK224_04225"
     CDS             27626..29038
                     /locus_tag="PK224_04225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB14975.1"
                     /translation="MAKPKVFVLTDDQAAVAAVRRSIESECEVFVAAWDRTAVVQIHE
                     QAPLVMLLDLGSSSDPAVEERVRVLQEVRQTGHAGKVIACTERTERRLAVRAIQYGAY
                     DVLSKPLDITLLAQLIRRAAGVADLEREARGVMVGGGPEEFNGMLGTSASIHRIFDAI
                     RKVSTNDAPILITGESGTGKELTARAIHERGLRQQGPFIPINCGAIPESLLESELFGY
                     ERGAFTGAVNQKKGKVEFAQGGTLFLDEVGELPNALQVKLLRFLQDHTFERVGGHQPI
                     EMNVRIIAATNVNLKEAIEKGTFREDLYYRLGVVHINVPPLRERGEDVSLMAMAFLRQ
                     AAAHYDKHLEGFTREALEAMRTYAWTGNVRELSNRVGRAVVMAEGTHVTPEDLDIPHQ
                     VARPDGSSISLKVNQQRIETDLIMKAFTLSQGNLSRAAQELGISRSTLYRRLRQYGMD
                     QTLDARRALGVFPRASLSEH"
     gene            29368..29934
                     /locus_tag="PK224_04230"
     CDS             29368..29934
                     /locus_tag="PK224_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRB14976.1"
                     /translation="MVSDAPALLSRRGLLRAATAGMALAVVRLALPSVTWASRLPDGR
                     VSLYNLHTDERLSVTYRDESGAYDQDALNALNHFLRCHHTNETTTMDVQLIEFVNLVQ
                     KRVGGRREVHIVSGYRSPEYNEQLIRMGTRAARHSYHVSGQAVDVQIPGVPLRTLREV
                     ALRLGCGGVGYYPRGKFVHLDSGPFRHW"
     gene            30218..30697
                     /locus_tag="PK224_04235"
     CDS             30218..30697
                     /locus_tag="PK224_04235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRB14977.1"
                     /translation="MMKVSKGDIVSVEYTIRLDDDRVIETTVGEAPLSYTHGQNEILQ
                     GLEAGLDGMERGAAKVITVEPVDAYGEIHPEGFFEVQRERVPAEAQRIGIKLETTAPD
                     GRVVFPYVAEIRPDVIVLDLNHPLAGRTLRFDVRVVDILRGERQLVTEGARGSEKET"
     gene            complement(30694..31131)
                     /locus_tag="PK224_04240"
     CDS             complement(30694..31131)
                     /locus_tag="PK224_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14978.1"
                     /translation="MLQRRDRRSFRPTAIGTLCLLGTLTMMAWALPVGVPYAAALVEV
                     TELLAHPDHYDHQVVTVSGRVSSFQLATNRDGHPAFGFLLQDTAGTVKVVGLGKADVR
                     EGDYVVVEGIFSRLRQAGRAIVYNEIKATSVQSMARMNPDLVG"
     gene            complement(31258..32427)
                     /locus_tag="PK224_04245"
     CDS             complement(31258..32427)
                     /locus_tag="PK224_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14979.1"
                     /translation="MTALLTPELIASTIEQAPIQGRIMLKLLLLQHFDITEEEITHIA
                     ADRPDPRCVAGSKPIHQVVKQDALRDVTSRRDEYRRRVRLRRERLWLQMEAMKRMIAL
                     AEGLIRAGSDLLLTQYKVDAAAVTALKQEARAAVPKPAIRLLNRRWDEDDISAEAYQE
                     ARLGLEIQTQLRLLDKYRKRFELSEREWKSINAAPLQDHEIGHIWGIPAGSLAARKVK
                     HLHQYLQAVQNSLQRSSAKDLPATAPLDLWKETLAVLGERPVERSIAQYDGLERTEDA
                     LVEKLRTFAWGTLGEDLEGKFWLSLVHGASSNAVHSENTRSLFGLQRLMAVLSEIDTS
                     PDALEQEMVTRTTPISKESLALPDEAAQPVVEEVGQMKQHVLTSFMGELHDDLRR"
     gene            complement(32461..32673)
                     /locus_tag="PK224_04250"
     CDS             complement(32461..32673)
                     /locus_tag="PK224_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14980.1"
                     /translation="MAENSAVYAIRFPDGSVTLYIDEEYAIDRGVDPAKLVRVEIPRE
                     LFASGSIQEIREYVAVYLENSHQGTA"
     gene            complement(32774..33436)
                     /locus_tag="PK224_04255"
     CDS             complement(32774..33436)
                     /locus_tag="PK224_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14981.1"
                     /translation="MSTHAPSYAIPDRRAAIYSALLPGLGQLIRGRYGAGLFYGLVTI
                     LLILLSLALGRVSGRAAEVFFFMLLALPWWALQSYDAALGPSESGSDLARSTRTAWAQ
                     GHDVRFLGLLFLVSAGNDALLIARNPDYLLPFFCTRLDGSAGFITKALSPFLHTLAGY
                     GFLRVKKWSLLIYLVYAAYGTTNALVNLACFGPGRIRNTLLIALLVFTSYVIARRRVF
                     RL"
     gene            complement(33540..34247)
                     /locus_tag="PK224_04260"
     CDS             complement(33540..34247)
                     /locus_tag="PK224_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRB14982.1"
                     /translation="MSSPEQAPRRQYVNFTFYKIDPAWRRLPEDERTRGKQEFLRAVE
                     EYQGKVLVIAYTTVGIRGDCDLMLWRISYELELFQEMSTKIMASGLGKYLYNPYSYLA
                     ITKRSIYVDHHTHENQESKRLTVVPGKAKYIFVYPFVKTREWFLLTKAARQGMMDEHI
                     EVGHRFPSVKLNTTYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTLRDTPI
                     FTCIRKSLKEALDTLGG"
     gene            34432..35004
                     /locus_tag="PK224_04265"
     CDS             34432..35004
                     /locus_tag="PK224_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB14983.1"
                     /translation="MGSLFVVLWGSTGSSYAIEVQPTTEQIQAQVERGKLAAGQGVSP
                     DQLHAWFGATEDLAPRGFIMTKLGSLLVMANHLALRALSPTEQDIAQVLANEHVLVNV
                     VLYGDRVNFAADSYIVLEQAGRTVKPSTVRFDARGDRTTAWPQQPAYRAKVIAQFAYA
                     ELDPRAKTTLTVFPSRGGQVSFELDFSRIE"
     gene            35052..36332
                     /locus_tag="PK224_04270"
     CDS             35052..36332
                     /locus_tag="PK224_04270"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRB14984.1"
                     /translation="MSKTKTWTAETIAVGSELLLGGRLDTNSLFIADRLAACGIELRY
                     KTTVGDDLSDIVAVLKTALRRARVVILTGGLGPTIDDLTREAVAQATGHRLSRRKAAL
                     DGMIARLVEWGRTPTTTQFRQAMIPARAEILFNPVGTAPGFSLVWKGTFLAALPGVPR
                     EMEPMVRDGVLPQLQAWMRTQKRLSASPMIRRVLHTSGVPESIVDQALVGLVPKGCTI
                     QLGIYASPMEVMVSLTGPAGAEGAVTIEQLLKEAKTRLGDIVYGEEDETMESVVGRLL
                     NEQQLTLAVAESCTGGLIGHRLTQVPGASTYVDRVAVTYSNRAKVEMLGVPSGLLKRH
                     GAVSADVAAAMARGIRERSGASVGVSVTGIAGPGGATESKPVGLVYVGLDGGPGEAMT
                     KEFRFHGGDRTVIKQRSSQAALDLLRRWLIKKGC"
     gene            36332..36949
                     /gene="thpR"
                     /locus_tag="PK224_04275"
     CDS             36332..36949
                     /gene="thpR"
                     /locus_tag="PK224_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRB14985.1"
                     /translation="MVRAFLAIELPQELRTTLATIEQDMKRQLERTVDRHVRISWVRP
                     DSMHLTLRFLGDTADESIEPLRVAIEESVSAHQAIPIPLERLGTFPRRQAPRVLWAGP
                     SVSWEQGLEAQRLTSLHRAVEACCRAANFAPEERPFSPHLTLARIKEGERQVGQALAQ
                     NGLLERPVAIGMLPVEAIVLMKSELRPTGSVYTKLWECSLAPSAT"
     gene            37233..39230
                     /gene="uvrB"
                     /locus_tag="PK224_04280"
     CDS             37233..39230
                     /gene="uvrB"
                     /locus_tag="PK224_04280"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRB14986.1"
                     /translation="MPPFKLEAPFKPCGDQGQAIEKLTSGILAGKQHQALLGVTGSGK
                     TFTMANVVERVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QSDTYIAKDASINDAIDQMRHAATTSLLQRNDVLIVSSVSCIYGLGSPEVYHDMLVYL
                     EEGMETRREKILAKLVDIQYARNDVDFHRGTFRARGDVIEIFPASSEAKSVRIELFGD
                     VVDAIHEIDPLTGKSLGKLPKIAIYPNTHYLIAPDRYERAITGIEEELDARVAAFKKN
                     GQLLEAQRIEQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGDPPPTLLDYFPKDFLL
                     IIDESHASVPQVGGMYEGDFSRKRTLVDYGFRLPSAVDNRPLKFAEFERMLKQVIYVS
                     ATPGPYELEHAKGEVIEQIIRPTGLMDPLIDVRSAKGQVDNLLAEVRTEAAKGNRVLV
                     TTLTKRMAEDLTEYYHDLGVKVRYLHSDIKTLERAEIIRDLRRGVFDVLVGINLLREG
                     LDLPEVSLVAILDADKEGYLRSHRSLIQTAGRAARNLDGRVIFYGDTITDSMRRAMDE
                     TARRRHIQEAYNEAHGITPESIKKQIPLLDYATGGGNAGQLELAAESVGDYATTGDTE
                     QLIRRLEVEMKAAAKKLEFERAAELRNRIRTLRLKALELAQ"
     gene            complement(39269..39529)
                     /locus_tag="PK224_04285"
     CDS             complement(39269..39529)
                     /locus_tag="PK224_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HRB14987.1"
                     /translation="MRKSIGVLLIFILIGGMLGGIFGEILRVMAPNGAIQNIFASNFS
                     PGINPPLTVDLVLLKLTFGFSIKVSLLSVLGMFLGAYLYKQM"
     gene            39954..41303
                     /gene="ffh"
                     /locus_tag="PK224_04290"
     CDS             39954..41303
                     /gene="ffh"
                     /locus_tag="PK224_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRB14988.1"
                     /translation="MLDALSEKFERILKKLRGQGVLTEDNIAEALKEVRLALLEADVN
                     FKVVKDFLDRVREKAVGQEVLKSLTPGHQVVKVVWDELRGMMGGERSGISLASRPPTI
                     IMMVGLQGAGKTTTSGKLARLFKSQGKRVLLVAADPRRPAAGDQLASLGHDLGIDVHR
                     FDQIDASRADVVRICQRGVERGQEQGYDLIVLDTGGRLHVDDELMAELVAVKQAVNPH
                     EVLLVADAMTGQDAVNMAGQFDQQVGLTGVILTKVEGDARGGAVLSIRAVTGKPIKFL
                     GMGEKLDALEPFHPDRMASRILGMGDVLSLIEKAQETFSREEAEAAQKKLTSSTFTLE
                     DFRSQLGQMNRLGSFEQILGMLPGGQKLKDLANSGLPEKEMKRVAAMIDSMTTRERRD
                     HTLINGSRKKRIARGSGTSVQEVNRLIKQFLQARKIAKVMSGGAGGRRQLAQMLRGM"
     gene            41410..41727
                     /gene="rpsP"
                     /locus_tag="PK224_04295"
     CDS             41410..41727
                     /gene="rpsP"
                     /locus_tag="PK224_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRB14989.1"
                     /translation="MAVHLRLARTGRHKRPMYRVIAADSRKPRDGRFLEILGIFDPLK
                     NPAVPVLKSERVLSWLRHGAQPTVTVRTLLKRHGVWKEFETEKSAKAEKTEKTEKSAK
                     SKK"
     gene            41738..42265
                     /gene="rimM"
                     /locus_tag="PK224_04300"
     CDS             41738..42265
                     /gene="rimM"
                     /locus_tag="PK224_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRB14990.1"
                     /translation="MATLDQAELVTIGRIERSFGVRGEARVRSLTDVPGRFESLGQVT
                     IVGPAGKTLDTRVTHVRPGGPTWIMGFEAFATPEQVAEFRGAFIQVPRGDSPALPADQ
                     YYQCDLIGMVVQDEAGTVLGCLEEVVSMSGNQNFLVRHDGKELLIPAAKQVVVAVDVV
                     GRVMTVRLPEGFGDL"
     gene            42267..43040
                     /gene="trmD"
                     /locus_tag="PK224_04305"
     CDS             42267..43040
                     /gene="trmD"
                     /locus_tag="PK224_04305"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRB14991.1"
                     /translation="MRCAVLTLFPDMVAPVLGQSILKRAQEKGLLEVSVQNLRDHTFD
                     RHKTADDVPYGGGAGMVMKAEPVLQAVDALRAHYQASHPETTLRVIIPSPQGRPFTQE
                     LAQSLAQDERVVVFVCGHYEGMDERVRLALHPEEISVGDYVLTGGELPALVMIDAAAR
                     LVPGVLGDAASAAEESFTEGLLEYPHYTRPAEVRGMAVPEVLVSGHHEAIRLWRRKEA
                     LRNTYLKRPDLLRDRELGSEDRRLLSEVMQESLVQVPVR"
     gene            43057..43470
                     /gene="rplS"
                     /locus_tag="PK224_04310"
     CDS             43057..43470
                     /gene="rplS"
                     /locus_tag="PK224_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRB14992.1"
                     /translation="MNRLERIQRSLTKKTTPKFEIGDTVRVHVKVVEGEKERIQVYEG
                     TVIARKGTLNSETFTVRKLSYNVGVERTFPIHSPNVAKVDVVRQGRVRRAKLYYLRTK
                     KGKFAKVEDREFKVESKAQTAAKREAAAAATVAKA"
     gene            43571..44233
                     /locus_tag="PK224_04315"
     CDS             43571..44233
                     /locus_tag="PK224_04315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRB14993.1"
                     /translation="MEARCRGYRHIAGLDEAGRGPLAGPVVAAAVLLPRRCRLPGLND
                     SKQVGESDRVRLFAEIVRRATGVGIGAATEAEIDRLNILQATRLAMRRALQALPLQPD
                     FLLLDAVTLSGLSVPQRSIIKGDGLSCSIAAASIIAKVTRDRLMVEYHRWYPQYNFAE
                     HKGYGTPEHLRLLREHGPCAIHRRSFAPVQQHCVGDARKLCSSVDQSESPLDRCATDA
                     DW"
     gene            44209..44595
                     /locus_tag="PK224_04320"
     CDS             44209..44595
                     /locus_tag="PK224_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRB14994.1"
                     /translation="MRDRRRLVGDEGEGRAEAYLRRQGFRILGRNVRSPLGELDLVAD
                     DQGVLVFIEVKRRRSGTYGGAIEAVDARKRAKLIQLAAQYLAQHRIHDRACRFDVVLI
                     QDDSGTTEPVQHIANAFDVAGDDLRW"
     gene            44698..45366
                     /gene="ftsE"
                     /locus_tag="PK224_04325"
     CDS             44698..45366
                     /gene="ftsE"
                     /locus_tag="PK224_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRB14995.1"
                     /translation="MIQLFHVSKYYDRRLALSDVTLEIEKGEFVLLMGPSGAGKSTLL
                     KLLIGAERPEEGQILVQGRSLSKLRSSEIPLLRRKIGVVLQDFRLLPKKTVFDNVSLP
                     LLVQGASTVEIRRKVTDALRSVGLDHKKDLFPPGLSTGEQQRICIARAIVNGPIMLLA
                     DEPTGNLDPELTSEIIELFKSINARGTTIIVATHDPNVLAQVNRRVITLDQGKVVSQE
                     QVCS"
     gene            45363..46262
                     /gene="ftsX"
                     /locus_tag="PK224_04330"
     CDS             45363..46262
                     /gene="ftsX"
                     /locus_tag="PK224_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRB14996.1"
                     /translation="MRRVLYLLREAVANVLTNRTTTVVAVATTAFTFACVGVFLLLYV
                     NLRTMASSLEQDIQVMVYLQDDLTEQTRNEIELQLKADRAVGSLTFVSKERALADFQS
                     QFPAESHLLQGLGQNPLPASFVVTLAVESRSSDAMRRWANRTQSIPGVAQVQYNQEWV
                     EALAGIVRYIEVAAIIVGLILSAASVTIIANTIRLALYSRREEIEILGLIGASTTFIR
                     VPYLLEGAALGLLGSALSLVILKAGFELFRHEIRSASRFLGVDALLTFFPFQMCVALV
                     CVGLFLGCAGSFLSLLRFGEGRA"
     gene            46259..47449
                     /locus_tag="PK224_04335"
     CDS             46259..47449
                     /locus_tag="PK224_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRB14997.1"
                     /translation="MKWARWGCVVVAAHLVLWTVPAWAERKDSYADKIEQEKKTLEKL
                     RGTIVEKRKKADEAEKKRESVLQGLQSLDERLVRYRQEHQDVVKKLKKKDVEIEQMSV
                     QLSRLSERIDERQDAIAARLRVQYVEGRFWHLRTLLAASSSRDFQRRFRYLSAVTQRE
                     YEIMETYRKDAERIAEVERSREEARQGILAYKASTEEKLAQIQGLKKQKRVYLAKITQ
                     EKESHDRAVEELERSANRVDSLLKELETRRRAALATRPPSATGGLRALRGTLLWPTEG
                     QVVSYFGRQKHPTFNTYIQRKGIEIRAAEGSNIRSVLAGQVVYADWLKGYGLVIIMDH
                     ANGVFSLYAHASKILTSVGARIEAGEAIGETGDTGMTGENTLYFELREGAEPVDPLVW
                     LSKR"
     gene            47476..48825
                     /locus_tag="PK224_04340"
     CDS             47476..48825
                     /locus_tag="PK224_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRB14998.1"
                     /translation="MEQRRSRRWLYLLLMVTVALGIGIVLEKGLERTGHASETYEELR
                     TFSEVLTQVQKHYVDDTKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEFG
                     GVGIQIGVKENRLAVISPIEGTPAHRAGIKAGDFITKVNDEPTKDLTLMDAVQKMRGP
                     KGTKVNLTIQRDGTADPLAFSLVRDTIKIESVKFKVLDNTIGYVRLTQFQEATGRDLS
                     RALKQFKEQKVQGTILDLRNNPGGLLTAAVDVSEQFVGNGKLIVYTKGREGKKDEWFS
                     KTKETLEDSPMIILVNEGSASASEIVAGALQDWGRAVIVGTTSFGKGSVQTILPLGDG
                     SGLRLTTAKYYTPKGRSIQSTGITPDIVVKLQTQTVAKAGEKDGKESEPKTAKAPAAG
                     KDPSPSAKPGEEPAHKNGAVSAGDGAGDISLDDDVQLQKAVELLKTWKIFKELRPAA"
     gene            complement(48800..49291)
                     /locus_tag="PK224_04345"
     CDS             complement(48800..49291)
                     /locus_tag="PK224_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRB14999.1"
                     /translation="MDDRTHQLAQIFRAMANLLASQRANPYRVRAYRKAADSILTVTG
                     DLADLAARHQLQEIPGIGRDLAVKIGEFLATGTIRAYEELKTPLPEEVAGWASLPGLS
                     DALVSYLYFRLNIRTLADLESLVASHLLRTQPGFSGSEDTLLAAIRERIGTVPTTPPA
                     AAP"
     gene            49420..49626
                     /gene="thiS"
                     /locus_tag="PK224_04350"
     CDS             49420..49626
                     /gene="thiS"
                     /locus_tag="PK224_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRB15000.1"
                     /translation="MIIHVNGESRGIGDGETVAALLHELEIRADRVAVELNLEILDRK
                     DFETRGLREGDRVEILSFIGGGAR"
     gene            49635..50414
                     /locus_tag="PK224_04355"
     CDS             49635..50414
                     /locus_tag="PK224_04355"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRB15001.1"
                     /translation="MDTINDRLVIAGREFKSRLWVGTGKYKDFVETKKAIEASGADVV
                     TVAVRRVNITDRSKENLLDYLDPKKYIILPNTAGCYTVEDAVRYARLARAAGVSDLVK
                     LEVLGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAA
                     PIGSGLGIRNPYNLKIIMETVKVPIIVDAGVGTASDAALAMEYGADAVLMNTAIAGAQ
                     DPIAMAEAMKYGVWAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            50544..51170
                     /gene="thiE"
                     /locus_tag="PK224_04360"
     CDS             50544..51170
                     /gene="thiE"
                     /locus_tag="PK224_04360"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRB15002.1"
                     /translation="MPSVDFRLYLVTDRQQTAGRPLVSVVGRAVSAGVRAVQLRERDL
                     PIRELLSLALDLQRELSDMQLFINDRVDLAVALGCRGAHLRESSLPAPVVRTLLQPSQ
                     LLGLSVHSIQDAVAAERHGADFVVLGPIYDTPSKREYGAPLGLQVLEQAARAVNIPIF
                     AIGGMTASRTREVRQAGAFGVAVLSSILSASNVEVATEKFLSAIERES"
     gene            51326..53095
                     /gene="arc"
                     /locus_tag="PK224_04365"
     CDS             51326..53095
                     /gene="arc"
                     /locus_tag="PK224_04365"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HRB15003.1"
                     /translation="MAESKGSSGRLRSFRDSVKRLTKSLSAGDGVVTHKNDEQAREVE
                     KLRIQIQSMEEEIRRLYQSRYQLEQTTKQNEKLVATLQEAKSQIETLRAEVEKLTAPP
                     STYGIFSSLDTDGTGNVYVSGRKMKVSLHPSIKAKSLRKGQEVILNEALNVIEVRGFD
                     VQGEVVRLKDVLEGNRALVTLHFDEEKVAELGEPLLSERLSVGDHLLYDPRSGCVIEK
                     LPKSEAEELVLEEVPDVDYEHIGGLQKEIEQVRDAVELPFLYPHIFANYKLSAPKGVL
                     LYGPPGCGKTLIAKAVASSIAKKLGHLKDKQLRSYFLHVKGPELLNKYVGESERQVRE
                     VFKKAKERADDGHPVIVFFDEMDALFRTRGTGISSDIESTIVPQFLSEIDGVERLTNV
                     IVIGASNRQDLIDPAVLRAGRLDVKVKVGRPDATAARDIFSKYVSTDLPFAEDDLKRH
                     GGDARALVDSLMNMTVDAMYATTDENKFIEVTYANGEKEVLYFKDFASGALIEGIVSR
                     AKKFAVKRAIAQEGAGLRAEDLIRAIREEFKEHEDLPNTTNPDDWAKVAGKKGEKIVH
                     LRTISGGPAEARQIETVNTGHYL"
     gene            53107..54606
                     /gene="dop"
                     /locus_tag="PK224_04370"
     CDS             53107..54606
                     /gene="dop"
                     /locus_tag="PK224_04370"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HRB15004.1"
                     /translation="MSDNPTHNPSRVIGTETEFGIASRDPNAADPVANSIHLIGYYPN
                     LPAPHAVWDYENENPLLDARGFEVDGERERPGPDYNRQLNKVLANGGRLYVDGAHPEY
                     STPECLNAREVVAFERVGERIVAQALEGITKARGRDQFVLYKNNSDGKGNSYGYHENY
                     LVSRAVPFERITQVLTPFFVTRSIYAGSGKVGAENQTSPVDYQISQRADFFETLVDLN
                     TMVRRPIINTRDEPHSDSAKYRRLHVIVGDANMAEVSTYLKVGTLSIVLELLEAGADL
                     PRISLADPVNAIKQVSRDVQVKESLKLSDGSSSTAIAVQRAYLKAAQSYYACHELNQV
                     TKDVLVRWEDVLDRLERDPRSLVRELDWVAKRYLIEFYMERKSCGWDDPRVRLMDFQY
                     HDVRPDKGLYYTLERNHRIERVVLDHEIARAEFNPPVGTRAYFRGQCIKKYPTVVYGA
                     SWTSVLFDTGQNKIKRIPLMDPLRGTESLTGELLAQAETASALLAKLST"
     gene            54620..55444
                     /gene="prcB"
                     /locus_tag="PK224_04375"
     CDS             54620..55444
                     /gene="prcB"
                     /locus_tag="PK224_04375"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HRB15005.1"
                     /translation="MKHSFFFSHHEGSSFFDFLTLHHPELRPGLSEIAIQRAALPVDF
                     GRPGTIPVPHGTTVLALKYRDGAIIAGDRRATEGFQIADRRIEKVFRIDDYSAMAIAG
                     AAGPCIEMAKLFQTELEHYEKLEGVQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYV
                     GYDLKRNEGRIFKYDITGGRYEESDHHSIGSGGKDARNTMREHYRSGLQEEEALRVGL
                     LALYNAADEDVGTGGPDLVRGIYPTAKIVSSAGITDVPEDRVRALFETMIAERRRS"
     gene            55448..56131
                     /gene="prcA"
                     /locus_tag="PK224_04380"
     CDS             55448..56131
                     /gene="prcA"
                     /locus_tag="PK224_04380"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HRB15006.1"
                     /translation="MPLPYYVSPEQMMQDKAEYAKKGIAKGRSIIALEYIDGILLAAD
                     NPSSSLHKVSEVYDRIAFAGAGKYSEFEHLRKAGIRHADLTGYMYSREDVSARTLSNA
                     YSQSLGTAFSTDVKPLEVEILVVQVGVNGQANEIFRISFDGSIVDEKNLAVIGGRSEA
                     VQQYLREHATPQPPTLKDGLRRCLAALEQVATQKIPSENLEVAVLDRNRLGRKFKRLS
                     SADISQLFA"
     gene            56263..57639
                     /gene="pafA"
                     /locus_tag="PK224_04385"
     CDS             56263..57639
                     /gene="pafA"
                     /locus_tag="PK224_04385"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HRB15007.1"
                     /translation="MQQRIFGLENEYGLIFSPNGKIYLPMEKVLGYIFEGLIPNSWPS
                     NAFLVNGARFYQDTGCHPEYSTPECDNILDLVVHDKAGERLLEACLPAAEERLREEGL
                     SGEIYIFKNNTDSLGNTYGCHENYLMRRDVDFWKVTEQLIPFFVTRQIYSGAGKVLKV
                     SGKPQYFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAEKYRRLHIIVGDSNMSEY
                     ATYLKVGTATLVLSMIEAGYSVSGMELEDPVKAIREISRDPTLNKKVKLDDGRQMTAI
                     EIQRVYVKRAKEFLSEQEHDPVLDDVWEKWVSVLDRLEEDPMQLVHEVDWVMKKHLIQ
                     SYTDKKDCGWDDPRVFLLDLQFHDVKRTRGLYYLMENRGMAARVVEEEAVQRAMSVPP
                     QTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRVEELLSQ
                     VSGARLYR"
     gene            57672..58658
                     /locus_tag="PK224_04390"
     CDS             57672..58658
                     /locus_tag="PK224_04390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRB15008.1"
                     /translation="MSFLDRFLIISVVLLTSVFPVELFPNTGPAPRGGDGQFSGKQGQ
                     VILINVSDIKDAASVKGQFLGRKVTLFPDPSADGGAGYVGLLGIDLQDEPGAHELTID
                     AQLGEQTRHFSYQVLVLKEKFAVEHLKLPKDKVDLDEKAAARWKAEQEQVRKALAEES
                     AMRLWQAGFIEPVHGKRTGIFGSVRIMNGQARNPHNGEDIGAPMGTDVMASNDGVVRL
                     IVDHIFSGRGIFVDHGLGLYSMYFHLSDVLVKEGDLIKAGQIIGKVGATGRATGPHLH
                     WGMKVNGARVNPYALLELPFPQNPAGARPVLASPTRPAQGESSATAVGGDTR"
     gene            complement(58730..61159)
                     /locus_tag="PK224_04395"
     CDS             complement(58730..61159)
                     /locus_tag="PK224_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15009.1"
                     /translation="MHVLLIEDNEDDAELIQQALCEQSTDAVTLNWADRLQTGIKKLA
                     ECPVDAVLVDLSLPDSQGLETLDRVRAQAHDAPVIVLTGLDNDVVAEKSLLHGAQDYL
                     VKGRLTGDALRRAIRYAMGRHRVEQALRKSEERFHLTCLATRDGIWDWEITSGVTWFN
                     EAHQSVYGSSPEKLRRGHIPWAEQIHPEDQAAILTNLAQVLQSDQNLWTAEYRFRRAD
                     GTYAYVIDHGYVLRDHEGHPYRMIGAKADITERRQAETMHAVQLAVGLALEESTSLSE
                     AVPKILRTMCELQGWTLGALWLVHSQDKTLRCNALWHQPGLPAENFARIYRSLALRQG
                     GGLAGQVWKTGDATLCPDILSETDCPILHAAQEADLHGGIAFPIHTGKEILGIMEFLT
                     YERLRPSSSRLERTSELGAMITQFLHRKDLERQLRQAQKMEALGRMAGGIAHDFNNLL
                     TIINSWAELLTDEPGQTTRTQRGMAQIREAGNKATALTRQLLAFTRHQMVERQPLNLN
                     DRVTDIVELMKRVIGEDIQLALTLDPALGRIKADPGQIEQVVMNLVVNARDAMPQGGR
                     LDLETKSISIAHPDPLWPDPLTPGPYVTLAVRDTGCGMDADTVAHVFEPFFTTKELGK
                     GTGLGLSTVYGIVRQSGGTVGIESEPGRGTTFTIYLPCIEEDSESPRPVAQSRSIIEQ
                     SETILLVEDNDMVRGLAQTVLVGQHHSVLPTRTGEEALQLVRQRGGKISLVITDMVMP
                     GMGGIQLAAELRTLQPEINIILTSGYSDREGSMLEQLDARTIFLPKPYTPDSLIKAVN
                     AALDPTLSNQT"
     gene            complement(61163..61603)
                     /locus_tag="PK224_04400"
     CDS             complement(61163..61603)
                     /locus_tag="PK224_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15010.1"
                     /translation="MTIRPFDILIAEDNEDDILLIREAFETVNMANRVAVVRDGEEAL
                     AYLRGEGPFSQCPFPGLVLLDINMPKKTGLEVLIEIKSDARFRPLPVVMLTVSEREED
                     IVRSFEQGACSYIRKPVTFSRFLSVVKEFELYWTLVSRVPTLKR"
     gene            complement(61600..64353)
                     /locus_tag="PK224_04405"
     CDS             complement(61600..64353)
                     /locus_tag="PK224_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15011.1"
                     /translation="MAIAENEALKSSTSDPFPRGRTILSLIVASIGIIFFLDVVTPLG
                     VPVWVLYLIPLILTYQAPVAWIPYLTAGVCAILTLLCLALSGPMTSMPSWMPLLNRAS
                     ALGMFAVIAHLIVQYRRATERLIQTAALDAEHKALQLKESLLLKNAQDVQDLYDHAPC
                     GYHSLNSQGMFVGINQTELDWLGYRKDEVIGIMRFQDLLTPESVELFHQQFPRFIERG
                     IVRDLEFEIRRKDGSLLPVLLSATAIKEPDGRFIASRSTLVDITERRKADVALRQSHQ
                     TLEVLVKERTAALEQTNRRLAQELAQSNRIQTALADSEGRFRELVECLPQPIWTCLPE
                     GECDYLSPQWMSYTGMPAAGQLGHGWTRQVHSEDRQMAIEQWQEALARAQPFDSEFRL
                     RRADGTYRWFHAHASPLRDNAGRLLRWLGTFTEIHDRKQAESMQARLAAIVESSSDAV
                     ISKSLDGRILTWNKSAELMFGYSGEEIIGQSIFLLIPSDRQLEETTLIELIKTGTRVQ
                     QYETIRRRKGGSCIQVSLTVSPIMDANGLVNAVSTIARDITARKQTEEMMTQQRRLIE
                     LSYEPIFAWEIDQGIVEWNRGCEQLYGYTRSEALGHSSNRLLQSRLPAPLGTIQAELE
                     QTGEWTGEIHHRTKDGRDVLVASRWGLLKTNGRSLVLETNRDITQLRQSETMILKKNK
                     DLETLLYVTSHDLKEPLRAIESFSVLIQERYANRLDEKGQDFLRRIVRATQRLDQLLT
                     DILDLSRAQRMDPPVDDIDAEQLVHEVLRRLEKRIKETDARITIRSPLPRLRVNTTWA
                     IQGIYNLVANALKFASAGQAPDIEIAPHSGMDLEGNELIGLVVRDRGPGVAPEQRDRI
                     FELFRRAVGREIEGTGAGLAIVRQVAERHGGRAWVEPREGGGSEFFITFGVNQSSPRK
                     VQR"
     gene            complement(64573..65994)
                     /locus_tag="PK224_04410"
     CDS             complement(64573..65994)
                     /locus_tag="PK224_04410"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRB15012.1"
                     /translation="MKLPTAHPPAPYDGSALIADPIHEYVSFTVPYATPDPSERTEKD
                     LIDSPWVQRLRYIYQLQSARWVYPSAEHTRFVHSLGTMHVAGRFARHLYPFLKKAVPD
                     VPSAALVEEFLRITALVHDIGHGPFCHFFDDNFLHGFGLSHEKLGQIIVREHLGPLIK
                     KIRRSPSGQFDRGEELNPDHIAHVILKEKGKDNSRIPRWINMLQPVISGSYTADNLDY
                     VLRDAYMCGVAVGPVDLTRLIHYTIVTDKGFTIHKTGLPALQMFLNTRMYLYSNVYYH
                     RTTRAIDIHLRDIFGDTMKLLFPSNPAKHMDEYLTLTDWSLLEDVRRWKKAGQSSLRR
                     LHQEWAHILGRDVKWKMAYSTVLKEKGIERGMDFPSHQQFQQQIQKALPAKLQTLPFR
                     VDMAPLDPRPDPKDTRGIPLLVFDPGTKGVSTEPLEEFLDLLPTRLVQFRIYALDHDQ
                     DAALSRAAATVLNKTPSSMETNF"
     gene            complement(66016..66495)
                     /locus_tag="PK224_04415"
     CDS             complement(66016..66495)
                     /locus_tag="PK224_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRB15013.1"
                     /translation="MADVRSLFRQVLHLDETPHRTALAFAIGSFIGFSPAYGLHMVMV
                     GFCAWAFGLNVVALLAGAFLNNPWTLIPILGGTYWTGATLLGVHDFPSFDWSDLSFMG
                     IYHQVMPYAWPFFVGGMVLSIAGGLLAYPLAYLLLSKYRRANPADGHQPLPPSSGLR"
     gene            complement(66519..67568)
                     /gene="thiL"
                     /locus_tag="PK224_04420"
     CDS             complement(66519..67568)
                     /gene="thiL"
                     /locus_tag="PK224_04420"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRB15014.1"
                     /translation="MPSGRSRRTSSVGSEFGLIRLLQAQAARPDRQVFKGIGDDTAIL
                     KTSSHEWTLITTDLLAEGVHFNPATSSYEDIGYRAAIANLSDIAAMGGTPRFMLVAIA
                     IPPTCSTQQIQRLYRGMMQAADPYRVCLVGGDTSASRYGLFISITLTGTVQPRRALLR
                     SGARVGDRLYVTGTLGDSRAGLDLLTATARAGAAKLRPTHTRFLLARHHRPSARIAEG
                     QWLVKHGLAGAAIDLSDGLTGDLRHLCEESGVGAEVLAGSLPISPACRAYALAHGRDP
                     QQIGLQGGEDYELLFTVPRRKQEQFEQLAEKTGFCFTCIGSITPKRLGLRLRTAAGTA
                     QPLPVTSYEHFLRPS"
     gene            complement(67570..70065)
                     /gene="lon"
                     /locus_tag="PK224_04425"
     CDS             complement(67570..70065)
                     /gene="lon"
                     /locus_tag="PK224_04425"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRB15015.1"
                     /translation="MAEAVEQDFSSNQNLEPPDQLPLLPVRDIVVFPYMVLPLFVGRE
                     MSIKAIEAALAGNRMLFLATQKSLDVENPQPDDIHAVGTVGIIMRMLKLPDERIKILV
                     QGLAKGRIEEYIQNDPYYSVRIEKLVETKQAGSTLETEAVMRTVKEQIEKIVSLGKVL
                     IPDVMVVIENLEDPGRLADMVASNLGLKVDITQAVLEIVDPIQRLRQISEILSKEIDV
                     LSMQQKIQAQAKGEMDKTQREYFLREQLKAIQKELGELDERAEEVAEFRKRIKDAKMP
                     EKVLKETEKQLKRLEKMHPDTAESATVRTYLEWMVELPWNKKSKDNLDLKAAMKVLNE
                     DHYDLEKVKERIVEYLAVRKLKEKMKGPILCFVGPPGVGKTSLGKSIARALGREFVRI
                     SLGGVRDEAEIRGHRRTYVGALPGRIIQGMKQAGTNNPVFMLDEVDKVGMDFRGDPSA
                     ALLEVLDPEQNSTFTDHYLGVPFDLTEVMFVTTANLMDPILPALRDRMEVIDIPGYTE
                     EEKLGIAQKYLIPRQMNEHGITEKHIRVNEATIRHVISHYTREAGVRNLEREIANLMR
                     KVAKKVAEGKSECHTIDQTNLNKFLGVAKFVPEAELEKDEIGVATGLAWTESGGDVLY
                     IEATVMKGKGQLTLTGHLGDVMKESAQAALSYVRSREKTLGISPDIFAKNDIHIHVPA
                     GATPKDGPSAGITMATAIASALAQIPVRRDLAMTGEITLRGRVLPIGGLKEKILAAKR
                     AKLATVVLPKRNKKDLEEIPKHILKGIELIFVDTVDDVIKAALRRSSSRKPRASAPKA
                     PVKKSARATTSKRTTGSPRKSLRLPTASPSLIL"
     gene            complement(70152..71093)
                     /gene="galU"
                     /locus_tag="PK224_04430"
     CDS             complement(70152..71093)
                     /gene="galU"
                     /locus_tag="PK224_04430"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRB15016.1"
                     /translation="MTRTDVRKAIIPAAGLGTRFLPATKASPKEMLPLVDKPLIQYVV
                     EEAVASGIEDIIVITGRGKRAIEDHFDRSLELEENLKGSGKGQVLNQMRHISNLANFC
                     YVRQPEAMGLGHAVLCAQHLIGDEPFAVILGDEIIDAAVPGLAQLIHIYKQRKGAVLG
                     VQEVPPQEVSRYGIISARRLGKGLHRVDDLVEKPAPADAPSNLAVIGRYVLPPEIFPI
                     LRKTPPGKNGEIQLTDALRQLVKHTPMFAHEIEGQRHDAGDKLGFLIATVELALKNPS
                     LGPAFGEFLQQRLRPTAADVRRRGPSRSGQGRTRTTN"
     gene            71437..73734
                     /gene="bamA"
                     /locus_tag="PK224_04435"
     CDS             71437..73734
                     /gene="bamA"
                     /locus_tag="PK224_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRB15017.1"
                     /translation="MPILILALVALYAFGVGAPLYAQDGVPTVTSVTIRGQKRIELQA
                     IEGRLTLKANDRFTADALREQVKILYGTGYFEDVQVETEPAAGGVSVGFVVREKPFIT
                     EIVFDGNEELSDDKLKEKVTIKSQTFLDQQQAKESAEKIRLAYQEDGYYNCQVIPVIQ
                     AVDEDRKRLTYFIKEGTKAKVLHINFEGMRAVTKEEIFKVTATREWIPWYGLITQLKV
                     PSLLSDAGVLKREELGNDIERMREVYLNKGYLNVQISQPTLELSEDKKWFEINYSIVE
                     GEPFIVQEVGFRGNTVFEDHELREGLNIRPGEIFQRAKIRGEITRITDLYGAKGYVFA
                     DVVPNVTPDNNSRTATILLNVKEGEMMRIREIHVNGNDKTRDNVVRRELRLDEQDVVD
                     TLALKRSFQRLNNLNFFETVEILPQQVDVDKVDLNVKVKEKPTGQFSIGGGFSTLDKL
                     VAIADITEGNLGGNGWLGRIRGQLGQQRSLGLVTFRNPYVNDSYNALQLDIYRSMTNY
                     ISYFESKSGVSATWSRWLSEYVNGSISLFGEQLKYSDPEAGLCPDFIPLICRQLGTQS
                     STGFRTSIFRDTRDYYLDPRTGWRFSLGVDYGTPALGGSNHFFKYYADVIKYTPLIYD
                     TRFSVHVRYGSTEGIGGRPIPLTERFFVGGINTMRGFVFGRAGPVTPNGSLLGSAKEL
                     IFNNDFIFTISSEAKLNGVFFFDYGNGFDDNEPVQFNKLRSAAGFEARWISPFGPLRA
                     AYGINLDPRPTERMGVFEFTIGSLF"
     gene            73773..74348
                     /locus_tag="PK224_04440"
     CDS             73773..74348
                     /locus_tag="PK224_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRB15018.1"
                     /translation="MGGPVGRSGSRGAGRAGLCLALVTVALVVGGCRSGHSTVPTSTR
                     IGVVDPQRVLSDTNAGRKAKDMLASFAKNRQALIELEEKELRRMEEDFMKQGSVLSAN
                     AKREREEQFRRRMSEYQQKVTDLNREVQDKQKEVLDGFREKIETLSGRVAKRLELQAV
                     FDRGRGGPTLYFDEAVDVSSQVIEEFNKTYP"
     gene            74360..74896
                     /locus_tag="PK224_04445"
     CDS             74360..74896
                     /locus_tag="PK224_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRB15019.1"
                     /translation="MNVRTWIVAGAVPVVLAWLAQPVQAADRIKVAMMDQQQVVERSV
                     AGKRALEDLKSYSTTRQKIIDSDDQELKELEKALQDSALTEEVRKEKQEQFRAKLEAY
                     QRRIQDFNREVQEKQKGMVLDFAQRIHAAVSAVAQKEGYTAVIDRGSESTVKIVVYGH
                     PSVDLTEQIVKEFDRQNK"
     gene            74939..75394
                     /gene="fabZ"
                     /locus_tag="PK224_04450"
     CDS             74939..75394
                     /gene="fabZ"
                     /locus_tag="PK224_04450"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRB15020.1"
                     /translation="MSVMDNVEIQSILPHRYPFLLVDRIRELDPDRRIVGIKNVTINE
                     PFFQGHFPGRPVMPGVLILEALAQVGGVLAFKSLGSVGRPVVYLTGIDGAKFRKPVVP
                     GDILRLEVDVLKKRAPFWKMQGRAFVESELVCEAEVTAMVTDEQAGGDK"
     gene            75463..76272
                     /gene="lpxA"
                     /locus_tag="PK224_04455"
     CDS             75463..76272
                     /gene="lpxA"
                     /locus_tag="PK224_04455"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRB15021.1"
                     /translation="MNIHPTAVVHPKAVLADDVEVGAYSVVGEHVRIGAGTRVLSHVC
                     IDGWTDIGERCELHPFVSVGGPPQHMQYKGEPTKVVIGHDNILREYVTVNRATVQGGG
                     VTSIGDANFLMAYVHVAHDCHLGNHLILANAASLAGHITIGDHAIIGGLSGIHQFVRI
                     GAYAMVGGCCALGQDLPPFMRAAGGYRARMYGLNSIGLRRHGFSSERIAALKKSYEVL
                     FRSGHRVAEAVKLARENFSASPDVMQVAAFMEGTKRGICRSVGKDQEGEEE"
     gene            76276..77118
                     /gene="lpxI"
                     /locus_tag="PK224_04460"
     CDS             76276..77118
                     /gene="lpxI"
                     /locus_tag="PK224_04460"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRB15022.1"
                     /translation="MGKQVAEHRQASDGQRIGLIAGNGRFPIIFADNAKRLGYSVSAV
                     AHEGETDPELARHVDHIHWIKIGQFGKLIEALKGDGVQRAVMLGGIKKTHVFSTVRPD
                     FRALALAAKLIHLKDDDILRRVAEEIEQEGIQICESTFGLEGILVEEGTLTRREPSKK
                     EWEDIRYGWDVGKQIGALDIGQCVVVKDRVIVAVEAVEGTDGAIRRGGDLAHGGAVVV
                     KRCKPQQDLRFDLPAVGPRTIEVMESVKASVLALEAGRGILLDRDETIAKANRAGIAI
                     IGMT"
     gene            77375..78364
                     /locus_tag="PK224_04465"
     CDS             77375..78364
                     /locus_tag="PK224_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB15023.1"
                     /translation="MKQLRAGVIGVGHLGQHHARHYATLPGATLVGVCDASPTRAKLI
                     ADRHGVQFWTDLDDLLKQVDIVSVAAPTSAHFSATKACLEAGKHVLVEKPIAVTSAEA
                     RELVELAARRGCLLQVGHSERFNPIMQRMRPFIERPAFIECHRLSAFGERGTDVDVVL
                     DLMIHDLDLVLSFNPGPVEEVRAAGVPVLSSNIDIANARIAFASGCVANVTASRVSTN
                     KMRRLRVFQRDRYVSIDFQTRQAVVSRRVQAAGAKPTIDIESYQAGDEEPLRLELDSF
                     IHAVNTGTRPVVSGEDGEAALNLATLVLEAIGRFTQRHSADEAAAVAFGRENV"
     gene            78404..79537
                     /gene="lpxB"
                     /locus_tag="PK224_04470"
     CDS             78404..79537
                     /gene="lpxB"
                     /locus_tag="PK224_04470"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRB15024.1"
                     /translation="MPRILIVTGEASGDLHGAHLVKALKELSPALQIVGVGGASMRAA
                     GAELVKDIPQLDVMGLIGLSAVKTMLQRISRIRTLIKGERWDLVVLIDNPGLNFHFAR
                     VARACGLKVLYYIAPQVWAWRRGRMRWIQQRVDHVLAILPFEEPLYKKAGVRCTFVGN
                     PLLDEVAPSYDRQALRRQFGLSDAGPVIGLFPGSRKGELLEHIPLLLETVKRLAQRHP
                     AIQFILAQASSIQDQFLADLLKDSPVPIRVFRNQASEVMAASDLLVVKSGTSTLQAAV
                     VGTPMILFYRASSWLTYRLARLLIRVPWIGLANLVAGRGIVPELIHDEATPERLVQET
                     ERLLGDPRAYEEMKAALLTVRQALGTPGASRRAAEAVLAECRA"
     gene            79534..81270
                     /gene="msbA"
                     /locus_tag="PK224_04475"
     CDS             79534..81270
                     /gene="msbA"
                     /locus_tag="PK224_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HRB15025.1"
                     /translation="MKHYLRLLKYLNPYRFRLGAAFLCALLVAGLSAAYAWLVRPVLD
                     GLFISKDESLLLVLPVAILAVAVLKGVFNYGQNYLMNYVGNQVIGDIREQLFSKLVRL
                     PVHFHDTNTSGRLVSRVINDVNQMANAVAGVLKDLFQQGLTFLAMIGVIIYQNWKLAA
                     VSMIVVPLSVVTMARMGKRLRNLATRGQERMGDMASTLQETLAGIRMVKSFGREEEEA
                     KRFRLSNDAFIHTTMKAIQVSSLGSSHMEVIGVLGVAGIIWYGGYLVIHDEMTPGAFF
                     SFLAAMFMAYTPIRRLSGANNTVQQALAAAERVFDVLDLPTEPAANGGQTDLPAISRS
                     LEFRQVAFQYEGQGEPALSGIDLTIRAGEIIAFVGSSGSGKTTLVSLLPRFYDPTGGQ
                     ILIDGVDLQTCSLRSVRGQIGIVSQEVVLFDDTVRNNIGYGRQGATPDDVMRAAQLAY
                     AHEFIVRMPAGYDTLIGERGLKLSGGERQRLAIARAILRDPPILILDEATSALDTQSE
                     RIVQLALTNLMHNRTTLVVAHRLSTIQNANRIVVLDRGRVAEVGSHEELLRKGGMYKR
                     LHAMQFADAISE"
     gene            81303..81965
                     /locus_tag="PK224_04480"
     CDS             81303..81965
                     /locus_tag="PK224_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRB15026.1"
                     /translation="MTTDRPAAAPQTQPLLKRVVNALKVAVVPPVGYQIIQMLRRTMT
                     WRTEGSEHVNRLFAEEKRVILAFWHAQQLMMPLAIPGLEAHVLISQHRDGELIRRIVA
                     RFGLDAVRGSSTRGGAEAFRQLIRLGRSGGNLVLTPDGPKGPRQVAKVGVVQLARATG
                     LPIIPMAFGCSKKNSSRAGIGSSCPIRSRGASFSWGRRSACRPTPRPTTLSMSAGSWK
                     TR"
     gene            82015..83370
                     /locus_tag="PK224_04485"
     CDS             82015..83370
                     /locus_tag="PK224_04485"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HRB15027.1"
                     /translation="MWYLLYNSLLLLVSPIILFVLLAKQRCRRGLPQRLGLRAEMPTI
                     DPAGRSGCIWIHAVSLGEVVAVAPLVRDLRRRYPETRLVVSTVTETGREAVEQRLEGV
                     AEHRYAPLDFPWVVNQAIDRLKPSLYIFVETELWPNILRSLRRRNVPSILVNGRLSTR
                     SFERQRLPVIRDFYRTMLNMISCCLMQSERDAQRMIDLGAEPSRVRCTGNIKFDQPVP
                     QAAAGGTAVSKAALGLTDRELLLVAGSTHPGEEETIVNAYRILSPEFPELRLALAPRH
                     IERAAQVEQMIQAKGLTVTRRSTGGQVPMASTGARVLVLDTRGELALLYRDAVVAFVG
                     GTLAPVGGHNLLEPAVWGKPVLFGPHTDHCAEVAALLLNAQGGLVVQDEQDLAQGLRA
                     LLRDPAALLRMGQAAQQVVADNQGALQRSAEIIATFLPARGTSKNARVEQGRELSSRQ
                     S"
     gene            83382..84452
                     /gene="lpxK"
                     /locus_tag="PK224_04490"
     CDS             83382..84452
                     /gene="lpxK"
                     /locus_tag="PK224_04490"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRB15028.1"
                     /translation="MSRGLRSWLRWVGYPYELAARLRLWGYTHGWFSTRRLPRPVISV
                     GNLTVGGTGKTPVVMYLVERLTAQGKRVAILSRGYRRRSNAPQLLVSDGQQVLVGPDE
                     AGDEPYLIARRCPQAVVAVGANRYALGQWVLGRLPVDCFVLDDGFQHVQLHRDLNLLL
                     VDATDAAGIQAALPVGRLREPLSAAARASAILITRVDEAHGGESVRCLLLDACGSLPS
                     LVRVGFRAEEFRRVGTGERLPLDAFRGRSAVLFSGIGNAESFRALVAGLGIAVIEMLA
                     FPDHVHYTRGMIDTIRAKAKASGADLLVTTEKDADKVAPLLVPEDACWAVRLRTEIVS
                     GQENLERLLRLDSAPGRTGGHA"
     gene            84445..86136
                     /gene="waaF"
                     /locus_tag="PK224_04495"
     CDS             84445..86136
                     /gene="waaF"
                     /locus_tag="PK224_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HRB15029.1"
                     /translation="MRKEGIRRIVVRGPNWLGDAVMCEPALSQVRTLFPQAEITLLVK
                     PGIADLLAQHPEVNRTLVYDDRGRHAGLVGKWTLAAVLRRHRFDLAILFQNAFEAALI
                     SFLAGIPRRFGYATDGRTLLLTDPVTVPPRTAQRHQVEYYWDLLKPLGGHGPAPAPRL
                     FVTPDESALIAGRLADAGIGPSDPVIGVNPGSTYGHAKRWLPDRYAEVVNRAVTDVQG
                     RSGARVGVAILGAKGEEPLGKAIAGQIKTRTVVCSGQTTVRELMALVKRCQLFLTNDT
                     GPMHVAAAFKVPLVAVFGPTDWQTTSPFGVDAQLVRQPVSCAPCLLRECPIDHRCMTG
                     VTVEQVYDAVVQHLPLVAPPPAVDPAPPTPGFTSTSLAGVTVFLDRDGTLNVDTGYVK
                     SPDDFTVLPGVGAALARLKQAGARLVVVTNQSGLGRGYFTSRDLEAIHSKLRLVLAED
                     GVTLDGLYFCPHHPDDHCNCRKPARGMIDRAHAELQVDLSRSYVIGDSVRDVELAKQV
                     GAQSLLVMTGPSGAEALADLTVRDLPPDYVAEGLSQAVDWIVAHATHRPAADMQR"
     gene            86154..87197
                     /locus_tag="PK224_04500"
     CDS             86154..87197
                     /locus_tag="PK224_04500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRB15030.1"
                     /translation="MTDSVRRLLLIKPSSLGDIVHAMPTLAALREQFPQARVTWLVKR
                     QWAPLVEVIAGVDQVCSVSEGLRGWLGRVPDLRAAGFDLVVDLQGLFRSGAMARLSGC
                     DRRIGFANAREGSSLFYTQRVAVPPAPMHAVDRYLLVAEALGATRPTQPRFEFVDRAE
                     DRQAVETMLSRAGVAPHQPWVAMNVSARWETKRWPPQHFAEAADRLSQAHALPVVLIG
                     GPAERAESLAVTALMRTKAIDLTGQTPVGLLPGLLRRASLLVTNDSGPMHIAAAVGTP
                     VVALFGPTDPVKTGPYGKGHVVLSHAVECRPCFRRDCAPAVPLECLTAVRPEQVVRAV
                     EQQLERSQKPGSL"
     gene            87194..88297
                     /locus_tag="PK224_04505"
     CDS             87194..88297
                     /locus_tag="PK224_04505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB15031.1"
                     /translation="MNIIIAESSTAVGGQELAVLLHAERLLKRGHRIRLVLEPRSPIM
                     AMARERGLPVEPFVMRQWRLPLSILAFRALLKRERPEIVHVNSSRDSWIAALSSRLLD
                     PRPKVIRTRHISAPLNNNATTHWLYRRLFDMVIVTGSERNRQDLIHRDGLAPDRVASF
                     PIGLDVEHFSPAKPQHDIRSELGISTGHLLVGMISYLRDYKGHRYLVEAAAKVLKQHQ
                     GVAFLIVGEGPEEQNIRAQIERLGLTAGVRMLGFRDDLLDVFRSLNLFVIPTVEGDTI
                     PQVLMQALAIGLPVVSTTTGSIPDVLADGESGFIVPPRDADALADRIGRLLVDPELRA
                     AMGRRGRQTVEQSYSIDRMVDELERVYRRVIAS"
     gene            88294..>88625
                     /locus_tag="PK224_04510"
     CDS             88294..>88625
                     /locus_tag="PK224_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15032.1"
                     /translation="MMQRWAEARASIRKSVLFWWILFVVMQMAERVFLLRDAFEQEAP
                     SLPLLLKTLVVGVRGDFITATFALVLAGVGAGAWALRPAPGRRTGRGAVLPDPPPPRH
                     RCGGAPGRG"
BASE COUNT        17343 a        24228 c        28571 g        18416 t
ORIGIN      
        1 acgctgaagt aaaagggaaa acgtgccgtg agttcatcca tcaaggggaa ctgcccgctg
       61 tcccgcctgg caaagaccat catgccggca agttgtcctc cgtgatccgt caccaccttc
      121 cccaatttgc tgacgcataa gccgcgagtg gtcacatcgt tcaaggagag aaagcgttct
      181 cccgtttcga ctgtgccttg gatgatgtcg gtgccgatct tgcccgtctc gtgattgaac
      241 ggggtcagga cgacgcggag cggcatgtgt ggagccagga tgtcgctggt tcgctcggcg
      301 agaaatcgcg cgtcggatga ggtggtgaga attcctgtcg gcggatggtt tcgaaacgtc
      361 tgcagaatcc attccgccat gttggatgcg atgatggtca cgagatcggg gaatcgcgcg
      421 atggattcaa agcgcagata gacacctgtg tggtggccgg agaccacttc cacatggcta
      481 tcgaaataca ctgctcgaga gtcatgcagc aggcgctgaa tatcccatga cgacagggtg
      541 gggtgcgggg tgtcgcgaat caggtgttcc agcctcacgt cgatctcaga accctcatag
      601 agcttcctct gtcgctccac caactccagg gtggcggttt ccggggattt gtcgattgac
      661 ggcatcatga cccgtactcc tctagttctg tgcggcaatg gtcgttctca gaatgccgat
      721 cggcagaatc tccaattcga cgacatcgcc tggtttcaaa tatcgatcaa gctccaggcc
      781 gcacccgccg ccgactgtcc cggagccgaa cagatcgccg ggatatagga actcacctcg
      841 cgacacgtgc tcgatcattt gcgaaaacga ccaatggatt gaaccgaaac gcccctcaga
      901 ccagacctcg ccgttgatac gggcgatcat ccgtaacgac gtgaggtcct gaatgtcatc
      961 gggcgtgacg atgcagggac cgatggcggt ggcgaaatct tttccttttg ccgggccgag
     1021 ccggcaggcc atttcctgaa attgaatgtc tcgcgcgctg aagtcgttca tgatcgtata
     1081 accggcgatg tagttcacgg catcccgctc ggcaatatcg attcccttgc gtccgatgac
     1141 gcaggccagc tccagttcgt aatccagttt ctgtgtgttc agcggccacc gaagctcatg
     1201 atcaggcccg atgatcgtcc ggtggttgcc tttgtagtac acgggggcct tgtaccattc
     1261 aggcgggatc ggttgtcccc gtcgcttgga ggtcgccgca atgtgctcct cgaaggcaat
     1321 aaaatcacgc agggatgaag gattggggag gggagccgtc aatcggacgc tcgccggatc
     1381 ataaaagatg gtctcacccg ccggacctgc gatggaggaa ccatggccta ccgcatagtc
     1441 gaatgcgcga cgggccgcgt ctgtcgcagc ggggccgcct tccaaaaact ccaacatggt
     1501 ggcggggacc tgcgcgtcgg cgaggcggcg cggttgtgtt tcgccctgat cggtgagtcg
     1561 tctcgcctgc gccatgttga ggtctacgat cgaggcattg tgcaacgcac cgattctggt
     1621 gaaggttccg acgggagtgc gcacctggaa gctgacgagt ttcatgggcg gctccaactc
     1681 atcgcatagt ccttcagttc aacggcgtca cattcgggca tcaccgtaaa ggcccgtcgc
     1741 gattcgatca tcacggcgac ttcgtccgtc tgctccttgg tcttgctcgc ggcgatggcc
     1801 tgcgggtgcg ggccatgatg aatcccttgt ggatgcagtg tgaacatgcc gggatgaatg
     1861 ccggcgcggc tgaaaaaatc tccctggtga taaaagagca cttcgtcata gtccatgttg
     1921 cgatgataaa acggaacgcg aagtgcgctc gggtcgcttt ccagcggcct tggcgcgaag
     1981 gtcgatatca gcagtccgcc ggcttgaaag gtcatgtgca cgctgggcgg gaggtgatag
     2041 cgcgggctga tgaccggacg aaaatcccgg acattcaact tggcgaccca gaggtcaccc
     2101 ttccaggccg cgacatccat gggatagaac gggtaataca cccgggtgta gtcaccgtcc
     2161 cgcttgatgc gcacttccca ttcctgtcga ctatgggcgt cttccggagg cagcgccagt
     2221 tccggcgcgg tgaggactcc cggatcaaat aacgcatggc ggccgagcgg gcctcgatcc
     2281 ggcacttcga caggcgccgt agtctcgacc atgagaaaca gccccggctc ttccttcgta
     2341 tcgacatgga ctcgatatgc tgtgcctttc gggatcacca ggtagtcgcc cggttcataa
     2401 ctcaagaatc cgtagtccgt ttcccaccga ccgcgccctc gatgcacaaa gacgatttcg
     2461 tccccgtcgg cattgcgcac gaagtgaggc atcgtttccg cacgtctgga cagggagaca
     2521 cgggcatccg gactctgcaa gaccagggtt gggcgtccgt cggcggaatg gcaatcgggg
     2581 gtcggcaatt cggtgcaggt aaacgctcgg gggcggagcg gaccgtcgat cctgctccac
     2641 gcagtcggtg gatggtggtg gtagaggtgc gaggaggggc cgctgaaccc ctgacgacca
     2701 tgctcttcct cgtggagtcc gtcgggaaca ccgacgtgtg cctgattggg aggggcgcct
     2761 tttttcacca agtacatcac gaacctcctg atgcctcagg gacggctcga tgttttacga
     2821 cgtcgactga aacgactcgg atggcgtgtc tttaaaggga agcggaaggg tctcgtagtc
     2881 gatgattgtt ttctcgatcc ctctggtttg atctctctca atgtctctat agagcgcctc
     2941 gacgtcttgg attcctccag cccgtgctgc cgttgggtca gttccaggaa caggccgcac
     3001 ccggggaaga ggggataggt gaatcgctgc ttgaggggcc cgaagccgtc gcggccattc
     3061 ttggtttctc cggagaatcg gactccatgg gattcccatt cgcgacagac cgcttcgatg
     3121 tcatcgacct ctagggcgat gtgctgaacc ccttcgccgt atttatcgat aaagtcacag
     3181 atctgggacc gacgtgcctt attgcggcct tgcatgagcg cgatcttcgc atcgccccgt
     3241 tgaacgacca cggtgtccat gctcgacatg tcactgccga catcctgagc ggaccaaatc
     3301 acgtgaaagc cgagtacggt ggtgaagagg gattcgacgg cggcgagatt tttcacgcag
     3361 agcgtgatgt gatcaatacc gatggcttct ttcatggtgg gtcctcctac gcggcctcgg
     3421 aaaacgtgga agggactcat gccattgtcg cgatcgggaa gagcaggatt cacacacgcc
     3481 cacactatat aacataatag ttgcatcatt caatatagat tttgtattat ttgccatggc
     3541 gacgaacatg agaaaggaca ttcgcatgaa gttcggttgg gacgagacgg gacatcccgg
     3601 tatgaccgcg gaggtgcacg atgtcgtttt acgaacatgt gcgcgcggac gtgctccggc
     3661 atggagcgat caataatacc tacctgacac ggtttcaagc cggtgaggtg accgacagcg
     3721 agttccacga atttgccgtc gagttttata gttttgcgcg attctttccc cgcatccttg
     3781 ccgctcaatt agtcaacact gaagatgagg ccgtggccga tgagctgacg aaagtcctct
     3841 actcggaact cggcgacggc atcgtcaagc atcgccacga attgctgtac cggaacttcc
     3901 tccggtcgat tggcatcgac attcatgaag cgatgcacac acccatgaaa cccagcacaa
     3961 gggcgtatat ccagggaatg gaacggcttt atagcagcag gcaccacgtg acggcgttgg
     4021 gagcgtcctt tggtcttgag aacatggcca tcacgatgtg ggaccatttg attccagggt
     4081 tgacccggtt gaagcaggtt cggtatccgc gcatggacat gacgtatttc accttccacc
     4141 gcaaactcga atccgcgcat gaaaaagcca tggagcatgc cgtggaagct gtcggaggag
     4201 cgggggcgaa tgggtgcgcc acgatgagcg cacaagagca aacagatttt tgcctgggaa
     4261 tgaatgcggt gctggactat ctcgaagggt tttggatggg gctggaacga aaggcgtccg
     4321 gtatccgacg gagccggcct cagccggcgc ggcagttggc gagtgaactg cgcggacaat
     4381 cgcattgacc cggctgcggg tcggaggatc acatggagcg cgtgtggatc agccgctacg
     4441 atgcgggtgt tcctctctcc tccgcctatc cggaatggac cgtcccggac ctgcttcgtc
     4501 gctccgcctc ctgttttccc gaatcgaccg ctctcttctt ttatggcgcg cgtctttcct
     4561 tccgtgaact gaacgatctg accacgcgtt ttgcacatgg gttgctgcgg ctcggcgtga
     4621 agaggggcga tcgggtcgct ctcatgctgc cgaatattcc acagacggtc atcgcctatt
     4681 acggcgtatt aaaggccgga gcgattgtcg cgcccatgaa tcctctgtac gtcgaacgtg
     4741 aaattcacag tcagctggcc gacgccggca gcgagattat cgttgcgttg gatctgtttt
     4801 atccccgcat tcaggccgtc cgcgagcaga cgggtctacc cgaacggatc atcatcacca
     4861 gcctcggaga ttcttccgac cgtgaagcgg ctgctctatc cgcttaaggc ccggctggca
     4921 aagcgctggg tggcggtcga gaaaactccc ccggtctatg actttcttgc actgctcgac
     4981 gcggcatccc cccgcaccga agatgattcc ccgcttctac ccacggtgga gccggacgcg
     5041 ctggcccaga ttcaatatac cggcggcacg accggcaccc ccaagggtgt catgctctcg
     5101 catcgtaacg tggtggtcaa tgcgatgcag gggcgcctct ggtgttcgga ttttcaggag
     5161 gggaaggaag ttttcctggg agcggtgccg tttttccatt gttatgggct gagtacctgc
     5221 cagaatctcg ccgtcgcgac tggttctcag atcgtcctgc tcccgcgatt ccatgcggag
     5281 gaggcggtga aattgattca tcagcaccgg gtcaccattg tgtcgggcgt gccggtaatg
     5341 ttcgccatga tcacagattg tccgaaagtc ggacggtacg acttacattc gatacgggtc
     5401 tgcctttgcg gagccagccc cttgccgacc gaggtgcagg aacgattcga gcggttgagc
     5461 ggggtcaaga tttcagaagg atacgggttg accgaggcgg gtcccaccac ccattgcaac
     5521 ccgattcacg gggatcatcc gcaaggctcg atggggttgc cgtttcccga tacggacgca
     5581 cggattgtgg atgaagagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnntgatg cagggctact ggaggaaaac cgaggacacg
     5701 caagcggtgt tgcgcgacgg ctggctctac accggagata tcgtcacgcg tgatgagcat
     5761 ggatttttct tttttctgga tcgcaagaaa gatgtcatca agccatgggg agagaccgtc
     5821 tatccgcgag aggttgaaga aatcctttat cagcatcccg ccgtgcgtga ggcggtggtg
     5881 gtgggcgctc ccgaccacca ttacggagaa gcggtcaagg cgtttgtcgt gcagaaggag
     5941 ggatgtgccg tcaccgagag agagctgatc gagcactgcc gccggtcgct ggcacggttc
     6001 aaggtcccgg tggccatcga attccgcact gaattgccac gcaccattat cgggaaggtg
     6061 ttgcgacgcg ccttgcgcga tgaggcggat ccggtcccgg ccggtatgca gtcgtcgcgc
     6121 aaggcgatgt gaggctgcga ggagggcacc atgagagagg tcgtcatcgt cgccggcgta
     6181 cgcaccccta tcggcaactt cggcggtgcc ctgaaggacc ttccgcctca caagatgggg
     6241 gaattggtcg tgcgtgaagc cgtctcgcgt gccaaggtgg atccccggct catcgacgaa
     6301 gtgatcgtgg gctccgttgg tcacacgagt gatgcctaca acgtcgctcg tgcgattgcg
     6361 ctcatggccg gactgcctgt gcgtaccccg gcctattcgg tgcagcgaaa ctgctcctcc
     6421 ggcctccagc cgttcgtcaa tgcgtaccag aacattcaaa gtgaggatgc agacgcacaa
     6481 gtcgtggggg gagtggaaag catgagccgc gccccgtttg tctcacggga catgcggtgg
     6541 ggaaaacggc tccgcaacgc cgaattgatc gacagtatct gggagggatt gaccgatgcg
     6601 ttttgtggcc aattgatggg ccggacggct gagaacctgg ctgaggagtt tggcatcggt
     6661 cgtgaggagc aggatcgata tgcggtggag agccaccgcc gggcgttcaa agccattcgg
     6721 gagggccggc tcaaggatga gattctgcct ctgatggtgc cgaagtcggt ggccggacgt
     6781 gatgtcgcac cggtggtcgt ctcgcaagat gagggaccga atgtcgggtt gacggcacag
     6841 caactcgcgc tctatccgcc gctcttcaaa gaacagggga gcgtcacggc ggggaacagt
     6901 tgccccctca acgatggcgc cgctgcggcg atcgtcatgt cctccgcgcg tgcgcgtgag
     6961 ttggggtgtc gtccgctcgg ccgcatcaga gggtatgcct tcatcggcgt ggagccgacg
     7021 cgtatgggga tcggtcccgc cgaagcgctc ccgatggcac tcaagcgcgc cggcgtcacc
     7081 ctcaccgatc ttgaactcat tgaagtgaac gaggcctttg ccgtgcagta tctcgcggtg
     7141 gaacgtgtgt tgggactcaa gcgggagctc gtcaatgtga acggcggcgc gattgcgctg
     7201 ggtcatccgg tcggcatgac gggtgcccgg ctggtcatca cgatcctgta cgagatgcag
     7261 cggcgtggcg ctgcactcgg tgcagtggcc ctgtgtgtcg gcggcgggca gggcgctgcg
     7321 atggtgttgg aacgcatgtg accggtgcgt gtcgagcgac aggagtcgtc ccatgtacat
     7381 ctataaagtc ggtgtggtgg gtgccggtac catgggcgcg cagattgctg aagtggtgag
     7441 ttatgccgga gtgccggtgc tgctggcaga tatacaggaa tcactggcca ggagaggtgt
     7501 cgaatctgtg cgggcgatat atcaagcccg cgtggacaaa ggcaagatga cgcctgagca
     7561 gctggaagag aaaatgctgc tggtgacggc ctcgccgaac ctcgaggctc tccatgatgt
     7621 cgatctggtg atcgaggcgg tctcagaaga ggtcacgctg aaacagcgcg tgttccgcga
     7681 actggaccgg gtctgtgcgc gaagtgccat cctggcgagt aacacctccg cgctctccat
     7741 ctcggccatc ggggcttcaa ccacgaggcc cggtaaggtg ctcggacttc attttttcaa
     7801 tcctgcctat gcgatgccgc tggtggaggt catccccggc ctggccaccg atccccagac
     7861 cgtcgatgat gtcgtcggtt ttgccgaaag tctccgcaag acgccggtga tcgtgaagga
     7921 atgcgcaggt ttcctcgtca atcgcctgct ctctccctat ctgaatgaag ccgtgtggtg
     7981 cttccaagac ggcgatgtgt ccatcaagga gatcgatcag gacatggtgg cattcggcat
     8041 gccggtcggc ccattcatgc tgctggatac cgtcgggctg gatattgcgt tcgaggtcgc
     8101 gcgcattctc caccgatcct acgggccccg gatggcgccg gcgccgcttc tcgaagcctt
     8161 ggtcaaggcg gggcgatggg gaatcaaatc agggcgaggg ttctacgagt attcgggggg
     8221 cgaggaacgg agcggcgatc aggacctcga tactctgctg cagcaagtgc ggcagggcgg
     8281 cgggcagacc aggatggaat ggaccagatt acgccctctc ctcgccatgg tgaatgaggc
     8341 tgtgactgcg taccaggagg gcgtcgcgtc ggcgcgagat atcgatctgg cgatggtggc
     8401 gggaacagga tttcccaacg agaagggagg accgttgcat gttgccgacc agcttgggat
     8461 cgaccatgtg ttgcatgaac tggaagcatt gcgggatacg ctgggcgtac gcttctggcc
     8521 ggcgccgatg ttgcgccgaa tggttgacgc cggcttcacc ggacagctcg cgggacgagg
     8581 gttctttgcc tattaacgct cttgtcggcg gggcaggatt agtatgctcg tccctttccc
     8641 gatcgcatcc atgtgggaat acccaccatt caatgctgtg aggctcctat gacgacgaca
     8701 gccggttcga tgctttcttg cactgtcgag ggggcggtgg ccactttgtt gatcaaccat
     8761 cctcccgcta acaccctgac acctgaactc cttgccgagc tgagcacgac gttcgaccag
     8821 gttgccaagg acgaagcggt aaaagtggtc gtgttgaccg gcacgggtcg attcttcatt
     8881 gccggggcag atattcgagt cttggcgtcg attgcttcgt caaaggaagg ggaggccatg
     8941 gctctgcagg gccaggccat cttggatcgg atcgaagagg ttggaaaacc ggtgattgct
     9001 gccatcaacg ggatttgcct gggcggagga ctggaattgg ccatgtgttg ccatatcagg
     9061 cttgccgcgg aaggtagccg attgggccag ccggaaatca atctggggat catgccggga
     9121 ttcggcggca cgcagcgact tgcccggatc atcggtcagt cgaaagccat ggagctgatc
     9181 ctgaccggtg agccgatctc ggcgcaggaa gcaaagacga ttggtctcgt atcgcaggtc
     9241 gtttcccctg aggatctcct ccgtcaggct caggggttgg cgcgcacgat tgcctcgaaa
     9301 ggccagatgg cggtgcgcgc gtcccttcgg gcgatccgcc agggtgtcga gctgaatctt
     9361 catgacgccc tggccttgga agcgcgcctg ttcggagagt tgtgcgacac ggcggacaga
     9421 cgagaaggcc tgtcggcgtt tctggagaag cggcagccgc atttcatgga tcgatgaatg
     9481 gccgttgagt cgatgtggag cccacgatgg caccgacttt cagcaagcgg gccctggcgt
     9541 tgggcggagg tggcttcacc ggctacctct tcgaaatcgg cgcgttgacc gccttggacg
     9601 atctcttcga ggacggtgtg acgatgaacg atctcgatct gtatgtcggc gtgagtgccg
     9661 gagcggcggc ggcgtcactg ttggccaatg gagtcaggcc gcaggagatt ctggacacga
     9721 atctgtccgg caccagaccc tactacttcg atcatcgtaa cgagttttct cctgccatcg
     9781 gcgaggggct gaaaacggtg tggcgggtga cccggcaact cgttccgctg ctgaagctct
     9841 atgtgcgtca ctatggagag atgacgttca tcgatttgct cgataaggcg caggatgcgc
     9901 tgccgagcgg tatttacacg ctggagcctt ttgcgaagta tctcgaggcg atcttcaaag
     9961 tcaaagggtt gagcgaccgg ttcgccggac tcgccaagga actgtacatc ccggccatcg
    10021 atttggaaac cggcgatggc gttctgttcg gcgacgagga ttggcgggac gttccgatct
    10081 cacgtgccgt gacggcctcc tccgcagtgc cgatttactt ctgcccggtc cggatccagg
    10141 gccgggacta catcgatgcc ggcatcggtc gcatggcctt cttcgagatc gccgttcaaa
    10201 aacacgtgga cttcatgatc atgatcaatc cgatggcacg cgctccgcag acgcggtcac
    10261 ggcaagcgca gccggccgtc ggcaagcccg gaaatcgatt gcgagacagg ggctttttgt
    10321 ccatcgggga acaggcctcc cgcatcaatt tcgacgcgcg tttctcgcaa gccttggagc
    10381 gcttccagca ggactatccg gagaaggaag tgctcgccat ttcgccggtg gaagccgatg
    10441 cgctgctgtt cgaacggagt tttctgagtt acgacgaccg gatccatctc cttcgggccg
    10501 gatatgtggc ggtgatgacg ctggcacggg atcggtggga ggacctgtcc gcgcagttcg
    10561 cgcgccatgg tctggtgatg tcgcgggcca ggttcgagga ccgcgccggg cggcgccttg
    10621 cgcggctcga tgaagtcgcg agggttgctg atccgaacac ccttcagccc atggtcggac
    10681 cggcacgcat cggcggtatc ggcgcggcct ggcgcaaggc caagtaagca acgccgcaga
    10741 ggtacgtatg acgaacggag cagagatgtt caaggggtcg ctcgcggcag cggaagccgc
    10801 tcgcgacacg cgtgcctatt caggattgat tgcagggcta ttcgaaggag tcgttcggag
    10861 atcgctgttt acctctgcat cgattccggc gatgagcggg gatgcgaagg aatttctcga
    10921 caccctacgc ctgctgctga tcgagaaggt agatccggaa gccatcgata gagaaggcgc
    10981 gatcggcgaa gaggtgatcg aggcgttcag agcgatcggc gccttcggga tcaaaatccc
    11041 gcgcagctac ggcgggttag ggctttcgca gtcggagtat cacacagtgg cgaccctcct
    11101 cggcagtcat gacgcggcga ccagcgtgtt gctgtcggcc cacaattcga tcggagtggc
    11161 ggaaccggtc aagctggtgg gaaatcgtga gcaacagcag cggttgctgc ctcgattggc
    11221 acggggagac atatccggat ttgcgctgac ggaaaagagt gcggggtgtg atatctggga
    11281 tctcaaaacc tacgcgattc ctgttcatga aggcggcgtc ctggtgggct atcggctcac
    11341 gggtgagaag ttgtacacga cgaacgcgcc tcgcgaggat catgagttcc tggcctcact
    11401 ccttgtcgtc attgctcaga tcgtcacggt gcctcaggac gtcgaccgtc ccaaggaaga
    11461 gcggcgattc ggggcgtttg tcgtggagac gcagtctgcg gggtgccgct gcacgcgtct
    11521 cagctttatg ggtgttcgag gcatctacaa tggccaggtg catctgcgcg atgtttttgt
    11581 gccggtagcc gaccggctcg gagaggaggg cgaaggcctg cggcgcgctt tggagagtct
    11641 cacggtcgga cgattgacac tgcctgccgc ctgcctgggc agtctgaaac aatgtctctg
    11701 gttagctcgg gctcgcgcgc agcaacgcat tcaatacgac cgcccgatcg gcgagcatac
    11761 ggatatcggt tccaaaattg ttctcatggc gtcccgcgtg ttggcgctcg aggccatcgt
    11821 gaagatcacg ggaatctgga ccgatgccaa ggtggatgta cggctggagt cggcggcggc
    11881 gaaagtcctc gcgactgagt ggctgctgga gtcgttgctg gatctgttcc gcatctacgg
    11941 cgggcgcgcg tttgagacgc ccgattcgtt gcgtctccac ggggacttgc cggtgccgat
    12001 cgaacggatg atccgggacg cgctcatcaa tgtgatctgg gaagggagca acggtattct
    12061 gacgttgtgg attgcgcggg aaggactcgg cgaatatgtg acgcaaggca gggcttttct
    12121 ggagcgcggg atcgaggaca tggctcgggc catgcccttt ttcgcgagaa tctccgcgcg
    12181 agccttcaat ccgattgcct ttcttgggcg aggtgtgcga tcgggggctg atgcggctcc
    12241 gtgggagcgg tttgtgtcgc aacaaagtcg ggcgctcgcg cggaaggcgc tgtgggtcag
    12301 cgcccgtgac cggcacaatc tcccgcgcaa tcaacgggtg ctcagaagcc tggtgacagc
    12361 cggcctgcag ctatttgccg tggaagcgtt gttgtggtac ggatcacagc cagaagtccg
    12421 ggagcatgcc ttgtcgcggg cgctggttga agatttctgt ctgcgagtca aagaaacatt
    12481 tcatcccacc ccgttgctct ctctgacgac gcccttgtgg gatgacgatc ggaggttgtt
    12541 tcatctggcc aaagacatac ttgcgggcaa ggccggatgg ttggaggagg gcatcatccc
    12601 gtcttgcccg ggcgggcgaa ccggtgttgc cgaaccggcc ggtgtggtga cgggcgcttc
    12661 ggtggagacc tggtcaggga tttgaggcgg agatgagagt ggttgttgct tcccaggatt
    12721 catgttcaac ggagtctcta tgacggaaat ttccgggtac ttcgaggcga ttaaagaccg
    12781 ctacgcgttg accagtgatt atacgctggc cgaaaagctt ggcattgcac aaccggatgc
    12841 caacttgatg cgacgcggtc tcaaggttcc caaaccggag ctctgcatca aaatcgcgaa
    12901 gttgctcgac aggaatccgg tggaactctt gctgatcgcg caaaaggaca aggccccgaa
    12961 acaggcgaaa gaatactgga ccctggcgct gacggccgtg gatgtcatgc tgcatgtgcc
    13021 gaaacgtccc cggtatctcc cgaagaaggt cgaggcaatc ggcagggaat tgaaacagct
    13081 ggagtcacaa accctgacct atgaaggcgc cgccgccaat gccgaggcgg tccgtctgat
    13141 ggagacggcg gagcagtccg tcgatgcgat gatggaacgg tggaacattt ggaagaaggg
    13201 ggaagccctg tatcccaact atctgttggc gaatcaggct gccgcacggc gccaggtcaa
    13261 aatcagacgc ctcctgatcc tcactcaggc gcagatgcag gacagcatga cggtgtccga
    13321 tgcggtgaac gtcatggacg atcagcaacg tgccggcgtc aaggtattct atgcgtttcg
    13381 ggaagcgctg gtgcagtcac ccacctttca gcggctcgaa gaggatttca ggaaacacgg
    13441 cgctgccgaa gacatgaata cggcgatgtt cgatcgggaa attttgatct tctcgcaaac
    13501 ctacggcacg gtcccgctcg gcatggtcgg cacgcccacg cccatcacca tgatcaatcg
    13561 gcttcagatt tcatggaaac cggagatgat ccgggacctg gatccggccc cgctgttcga
    13621 tatgacccgc tatgtgttcg agtatgaggg ggtcggttca ttccgggaac agctggcccg
    13681 gttcacgcgg tcgatgcggg agcttccccg ccgagccgtc tagcaggctg cggaacaact
    13741 cgattgtcgc gcaatacggt ctgatcaact cgtgcgccgt gtcggtatca gcatcacccg
    13801 caggatgcgc aaaaaggccg tctcctcgtc cgttgttcgt gaaacgtcgt tcgtctctcg
    13861 gcgtcagaaa agagcgtatg gcatagagcg catagcctat ggctagaaat cgggagtggg
    13921 cgtccgtact ctcagctata cgccaccagc catacgctct gaccctgacg agatacgctt
    13981 cacgattcac gcttcacggg tgtcgcgaac gccgctggcg gactttttcc gcatcctgct
    14041 agagtcaagc ccgccgcatc catccgatat caccccacat gaccgacggc cgtatcgtgt
    14101 cgcaggccga gccatgcgct cactcatagt gggctgcggg cgttcagacc tggaacgcaa
    14161 ggtctctccc gccggccttc tctccgccgc cccctcacga atctccctta tgaggatcga
    14221 cttcacccgc acgaaaaaac ttttcgaggc aggagtattt taattcgaga catgttataa
    14281 gcagttcctt cgcaggtaag actctccaaa gagatacgta tcgcgcggct gtctcattgt
    14341 cgcacaggca cggttcttcc gaggagaggc acatgggcgc aacaagagac atcgcgcgga
    14401 tgcaagggca atgagggtgg tctggattgt tgcgggcagg gggacagcca cgctgtgccc
    14461 cgtcatcaag aaagagggga ggccttgatg gcttgcgtga agaagtcggt ggggcaggac
    14521 gcaggggtga atctgtcggc tggtgcgggt ggggctgtgt cgggtggctc atcccatcct
    14581 cggaagcact ggcaattgct acggagatgg cgtcgcacat ttgcgagtgt gacggctctt
    14641 ctgttgcttg gggtgctggc attgccggcc tattcgcaga ccggctcgca actcggtccg
    14701 ttgcccccga aattgcccca attgcctgtc gtcccgcctc ccgcgcagcc tccagctatt
    14761 gaggtgccgg caccccgccc cccaagtgca ctcgatcagg cggccaaggg tcccaagatc
    14821 gcggtgaagg agatccggct gatcggcaat accgccttca ccttgcaaca gctctctgaa
    14881 gtcacctcgc cctataccaa tcgcgagctt acggcggagg acctggaatc gttgcgcctg
    14941 gcgctcacgt actactacgt caaccatgga tatttgactt ccggtgcggt ggtcccggag
    15001 caggatgtcg cggacggcgt cctgaccatg cagatcatcg agggaaagat cacacaggtc
    15061 aacgtcgagg ataccaagtg gttccggccc tcctattttc agagccgggt caatctggca
    15121 gccggtcctc cgctgcatgt cgaagcactc caggaacggt tgcgcgtgat gcaagccaat
    15181 ccccggatcg aacggatcaa cgccgaactg ttgccgggcg cgaccctggg agagaatacg
    15241 ctgaacgtga aagtgaagga agccaatccg ttgaaggcct ggctggagtt caacaactac
    15301 cagtccccgg tcgtgggtgc ggagcaaggg ttcatcacgc tggctcacca gaacctgctg
    15361 ggcttcggcg atacgctgag tttgcaatac gggcggtcgg ccggggtgaa cccgatgctc
    15421 aactttaaat acgaaattcc ggtaggcccg cgtgacacca ccgtcgcgtt gcaatatcgc
    15481 cggttcgatt tcggggttga ggaagcgccg ttcgatgcgt tggatatcaa gaataaggct
    15541 cagattttcg ggctgtcggt cagacatccc gtcatccgga cggccgacca ggagctggcc
    15601 ttttccctca cgggcgaaca tgcccggaat gaaagtacgt tgggcgggaa tccgttcgag
    15661 ctcatcaccg ggtcgccgaa cgggaagttc cgcgtgacct cgctccgctt cggtcaggaa
    15721 tatgcccggc gttctgccga tcaggtgatc tccctcttgt cacgtttctc cgtcggtgtc
    15781 ggcgcgatgg gagcgaccgc caatggtgat ccgaacctgc cggatgcgcg tttcttctcc
    15841 tggttgggcg aagcccagtg gatccgccag ctccccttgt ggcggacgca attggtgagt
    15901 cgaggcgtgg tgcaattgtc caacgatcac ctgttcccgc tcgaacagat tgcggtcggt
    15961 ggccggtaca gcgtgcgcgg ctatcgggag ttcacgctga ttcgtgacaa tgccgctatg
    16021 ttgtcgattg aggcgcgggt tccggtgtat acgacgaagg ctggtgtgga ttcggtcttt
    16081 ctcgcgccct ttttcgattt gggtcatggc tggcaaacga cggcccagac gcccggtacc
    16141 cccccgaaaa cgttggcgag tctgggtgcc ggcgtcatct ggaatttttg gcgtgggagt
    16201 cattttgaac tctactatgg aaaacagttg aagcggtacg ataccgaccg caacaacctg
    16261 caggatcacg gcatccatct gcagctggtg gtcgaggcgt tctaggggcg aatagccagg
    16321 gccgatggat ggtgtcttgg atgtgtcgtc tcacggagta aggggttatg cgtgttgaag
    16381 ttgtgcaccg tttagggtaa gagaggaggc cttcctctct tatcacacga ggagaattac
    16441 catgacgtgt cgccccgcag gtctgccgct attcattcat gggttactcg ccttcctgat
    16501 gcttctgggc catacggggt cgatcgccta cggacaggcc acgaacatcg tcgcgacccc
    16561 ctccggacca ggcggtcttg ggacctccct gagcacctcg ggcaatacca cgaacattac
    16621 cggcgggacc agaccgggaa gcggtccgaa cctctttcat agcttcaatc aattctccgt
    16681 cggcacgggg gatgtggcgg cattcgtgaa tcccggcggc gtgtccaacg tcatcagccg
    16741 ggtgatcggg ggatcgccat ccaacattaa tggtacggtt caggcgctca acgcgaacct
    16801 gttcttcatc aatccttccg ggatcgtgtt cggccccagc gcttcactga acgtgtccgg
    16861 gtccgcctat ttcagcacgg ctcagcaatt gcgtctgagc gatggcggaa tcttcacggc
    16921 caacaccgga ctcctggcct tcgactcgac cttgtcggtc ggctcacctg tttctttcgg
    16981 gttttttggg cagggccctt acggttcgat cgtcttatct tctgcctcga cagtgctcca
    17041 gaccggggcc gtactcggct tgatgggggg cgggattcag atcaatggat caaaaatcag
    17101 cgcgcaacga gtcattctcg gcagtacgag ctcggccggc gagatgagtg tccagccgtt
    17161 tgtcggcaat cctttttccg gcgcgtcagg aaacgggcag gtccaagccc tgcctgggac
    17221 cctgattgtc gcgggtggtg gaggagtgat tcccgcctcc atcgatgtga ccccgaatgt
    17281 gagcgtgccg acaggagcgc aggtcaacac gacgacgaat ccgttgaatc aacgtgtgac
    17341 gcaaatccag gtcgtgggtg tgaatcttgg cgggcttccg cctcttccta cagcgaatgc
    17401 ggcaagtgtg aatacggcta atcccgtcga gcctgtggcg ttcttgaatc gcgccgtggt
    17461 gctgctccct caggatgctc cggccccacc ggccccgctg ttgaccagcc gctgcgcggc
    17521 acggaaggac ggcgcattca gcagccttgt tcaagcctcg cgcgacgtca cgccgtcgca
    17581 accaggcgga tccctggcgg cgcctgtggt gctggaggag gtcggcagcg aaggcgagcc
    17641 gggcgctccg gcgacacaaa cagcacaaag actgggtcag tcgacggcac aacttatgga
    17701 atcatggcag gggtgctagc tccccaagga ggacgtcatg cctacgaagc gtaacatggt
    17761 cactcggcgc ggcatgaagc ggaggcatgg atccgctggt ccctccgcca cgcgcgcgct
    17821 ttcctcgccg ttgacgatgc tgggcgtcgg ggtgcttgcg ttgagcggat tggcctggcc
    17881 tgtgagcggg atcgcgatgg aattatttgg cggttcgggt gcgtcgtcga tctcgccttc
    17941 gcaagaaatg agacagggga cgacacagtt tcagcagggt gcgtttgccc aggccgccgc
    18001 gcactggatg aatgctgcgc gcgggtatga agagagtggg caggcaaaag agcagtgcca
    18061 ggcgttgatc aacctcgcac atgcgcttca gcaggaagga cagatccgtc gcgcgcaagg
    18121 cacgttgcaa gccgcgctca aactctcgga acagaatggt gagcgtggcc tcacggcgac
    18181 gattctcgct cagctcggca acacctttca cgtgctcggg aaagacgaac ccgccaccga
    18241 acatttgacc agagccttga cgttggcacg tgaggagaac aagcctctgt tggttgccgg
    18301 ggtcctgaac gatctgggta atgctctgac ggctcgtcgg cagtttgccg aagcgatcga
    18361 cgtctatgcg gaaagccgga gcctcgccat cgagaccaag cagccggcac tggcggcaac
    18421 ggcccaaatc aacggcgcga tggccttgct ccagaaccaa caactgagtg aagcgcatcg
    18481 tcatttggat caagcctggt cggatgtgcg gtcgctgggg gatggtcagg cgaagacggc
    18541 cggtctgttg aatatcgggc ttggctatca ggagctgttc gccgccacga ccgcgaaatc
    18601 aggcgtcgcc aggaaatcgg atgcggggaa gggccgcgcg acggaggcgg ttgcttccgc
    18661 cgggctggga gccggcctgt tacggcaatc gtccgatgcc tttaccgcgg ccggtgaagt
    18721 ggccggacgc accggcgatg cgcgtggaca atcgtacgcc tggggctatt tgggtggatt
    18781 gctggagcag gagcatcgca atcccgaggc gttggagtat tcacggaagg ccaccttcgc
    18841 agctcaaaag gtgaacgcgc cggaatcgct gtaccgttgg caatggcaaa cggcgaggct
    18901 gttgcgtgcg gccggcaaag aagaagaagc actggccgcg tatcagcgtg ccgtgacgct
    18961 gctgaagccg attcactacg aatattccgt ggggtatcag ggacggcacc actcgtacta
    19021 cgagtctgtt gcgccgctgt ttgtggagta tgaagatgtc ttgttgcgga gagcggcggc
    19081 ggcgaagacg cccgaccaga atgaacagtt gcttgttcag gtgaaggata cggttgaggt
    19141 ctcccatgcg gccgagctgc aggattattt ccaggacgat tgtgtgacga cggtcgcgag
    19201 tcatcgaggc gtcggcacgt tggcgcccgg caccgccgtg gtgtatccga tttctttccc
    19261 ggaccggctc gaactgctcc tggaaactgc caatgggctt aagcaggtca gggttcctgt
    19321 ggcgggagag aagctgacga aggaaattcg ttcgttcagg cggctgatcc aggattccca
    19381 atcgcagaac tacctgtctt ccgcgcagac gttgcatggg tggctggttg cgcctcttca
    19441 gcaggatttg cagggagccg ggattcatac gctcgtgatg gtggccgacg ggtcgctccg
    19501 gactattcca atgggggcct tgcatgatgg gcggcatttt ctcgtggatt cgcttgccgt
    19561 ggccgtcacg ccgagtctgg cgttgaccga tctgagtgct gcccagcgcc gaaaaggcag
    19621 tctgctgtcg gtgggcctga ctgaatcggt cgagggcctg tctgctccgc ggtacgcgga
    19681 gtccgaagta caagccatca gaacgctgta tggcgggaag ctgctgatga acaagcagtt
    19741 ctctgcaccg tctctggagg aagagatcaa ggaccaggga gtggggatcg ttcatgtggc
    19801 gtcccacacg gtcgtcggca ccgaggccag agattcgttt gtgctggctc atgacggcaa
    19861 gatcacgatg gaccggttgt cgcagctggt gggtcttcag caataccggc agcagccctt
    19921 ggatttgttg acgctcagct cctgcgagat tgctgccgag gacgatcgtg cggcgctcgg
    19981 gttgagtggt gtggccgtca agacaggggc gcggacggct ttggcgagtt tgtggacgtc
    20041 ggatgatgaa acgaccacgg aactcgtgtc ggaattctac aggcaattgc aggatcccgc
    20101 gctgtcaaag gccgttgcgt tgcaacgggc ccagcaaaag attctttccc agcgcggtca
    20161 cacgcacccc agcttctggg cggccttcct cttgatcaac aattggatgt gatcggggca
    20221 ggattcaagg gcctctcggc aaaacgataa caagccatcg atcctgtatc gatggcttgt
    20281 tcattggcgc gaatcaggag tgaggagagc gctgccggag acggcacacg gccctggggg
    20341 agagggggaa aggctagagc tttttcccgg tcatgatttc gtaggcttcc tcgatcgtct
    20401 ccacttcacg caccaacaca ttcgattcga ggttcaggct gactcgattg actcgcgggg
    20461 cataaaattg ccctcgcggc accagcagta cgtggtagcc agccctcgcc gccgccgtcg
    20521 ttttttcagc cacctgcgcg acttgtccga tccggccatc gccttccagc gtaccggtga
    20581 tcgtaatgtc ctgtttcagg tggtcacctt tcagcagggc gagaaacccg atggccaggg
    20641 cggcttctgc ggtcgctccc tccgcgttca atggcccgga aaatctcgca tagagggaca
    20701 aggttccggt cggacggacg gatggagcca ggcgttcgac ggcaaagcga aaggcccgct
    20761 gaatggaact catcccggtc cctctgacag gaacctgact ccgcccccac cgcaacgaca
    20821 atggatccgg ttggcttcct tcatcccatt tcatcacaaa tgtcgggaag ccgacttggt
    20881 cgtcagtgta ggtggcgatg gagatgggga ctgtgagggt tcgacttgca gaggcggcag
    20941 agggaagcaa tgaaagcagg gcagtcaggg agaggacgac tcccgcaacc atgcctgaaa
    21001 atgtgccgct tctgggacat gttgttcgcg cgcgcatgaa ttaagtctag atgaggatgc
    21061 tcgattgtca aatcgactcc gcgccgtcat tgtgcgcagt gagagcgtgc gggaatgagt
    21121 ggggtaaata tccgaaaaca ctcatctctt tttctgaatt ctggaattac ctactgaaat
    21181 gcgtgaggag gcgggtcatc tggacccatg atgcctcttg cctcaggcgc gtgatattct
    21241 tgcgtcttgc tcatccgctc gcccgggcat gtatggcagt cagtgctgcg tggagaacag
    21301 aacgtatggc cgtgcaggga ccgattgtga tcaccggagc gtcctccgga attggtgcgg
    21361 cgtgcgcgca gtatctcgat gcgttggggt tcactgtatg ggcgggagtc cggagcacgc
    21421 aggacgggga ggcgctgaca cgtctgacct cctcacgact tcgtgtgttg atgctggacg
    21481 tgacggatcc ggcgtcgatc gaggcggccc gcaagacgtt ggccgaggct acgcgtgaca
    21541 cgggactggc gggtctcgtg aacaatgccg gtatttccgt cgccggcccc ctggaactcc
    21601 tgcctctctc agaggtgcga actcagtttg aagtgaatgt gatcggcgtc cttgcggtga
    21661 cccaagcact gctcccattg ctgcggctgg cgcgcgggcg gatcgtcaac atcagttcga
    21721 tcgccggcct tgcggccacg ccgtttctcg gcgcctactg cagctcgaag tttgcgttgg
    21781 aggcgatgag tgatgcgcta cggcttgaat tggcgccgtg gggcatctcg gtgtcgctgg
    21841 tcgagcctgg tgcgatccag tcgcagatat ggcaacgggc tacgatgtcg gccacgcgca
    21901 ccctcggtgg tgtcgagccg gaggcactcg ctctgtatgc tcagccgttg tcccggatgc
    21961 aggaggtgat ggccgccgcc gccgcgcgcg cgattccggc agaggtggtg gcacgggtgg
    22021 tggccgacgc attgatcgcc gcgcgaccac gtgcgcgtta tctggtcggc aaggatgcgc
    22081 gattcagagc cacgctgaaa tggatcttgc ccgatcgcgc tcaagatcgc ctgttggcct
    22141 ggtttctagg attgccccac cggggctaga tggagtaagt cagacggaat gtcgagtgcg
    22201 gcggggggaa gaacgggtta tgcctgaagc aggattggtg cgatcagcgg gaggcgtggt
    22261 gttccggcaa caggatgtgt tgctgatccg tgtgtccgat atcaaggggc gtcccgtctg
    22321 gtcgttcccc aagggccggc tcgacgccgg ggaaacgcct gcgcaggctg cgttgcgtga
    22381 ggtgttggaa gaaaccggat ggtgctgtcg aatcgaggcg gacctctcca cgaccgaata
    22441 ttggtttcag cgggaggggc ggcggttccg caagacggtc gtgtggttca agatgtcggc
    22501 gcttgaagag gcgggagtcc ccgacggaga ggtcgaggag gtgcagtggg tcgatcgcga
    22561 agtagcactc ggccgtctga cgtatccatc ggacgtggcg ttgttgtctc aagcgctttc
    22621 ccaggggcct tcctcccgat agccgcaatg gtctgcgtgc gagacgggtg tgagatgcga
    22681 tcgcttgaaa tgattcgggt gtttcggtca gcctgaaccc acctgtctgc cttgacatgg
    22741 ccggccccct ttactagact cgccacgaga gtagctttgt gcctcgcctt gcaaggcagt
    22801 gctctgcaga gacgcgcctt ccctcacgag tcgttttctc cggacgaacg tcaaccagtg
    22861 gcgcgttgat ccgtattccc ccatcgttca cccgagggcc cgcctgtgta tagtctggct
    22921 cgcttttccc tgttggaaat gaccgaatgc tctgccgtgt tgcgtcagct tggcgagcaa
    22981 tcgagttcgc ttcaggatgc cgcatcgcga gcggtggact atctgttccg caccctcggc
    23041 aatcccgaca caggcgctcg cgactgtgtc ctggttcgct gcttcaagac cgtttcctac
    23101 aaccggttga atccgatgac ccagcagagg gttcgggaac agctcggggg catcgctccg
    23161 ttccccgacc tcaagtgttt cacgctcatg gccacggccg gtgaggaggc ggagtggaat
    23221 caagtcgagc gttcccatcg ctaccgggcg attccgatgg tcagcgccga tttcgtcaaa
    23281 cagtttccga tgttttccca acttttgcac caattcggcc tctcctccag ctttgatgtg
    23341 gctccggagg gggagtggtt agtggatctg gaggagaaga cgtacaacgt attttatgtg
    23401 ccggacgcgg tgggaagccc ctatgtgccg gtgcaggaag ggtttgtgct gaagtacggg
    23461 gtgaaatcgg tgttgtgttt cggtgggatg ttaccgtccc gagatctctt cgccgtgatt
    23521 ttattttcca gaacaccggt gccccgtgac acggcagcct tgtgcaagtc gctcgccctc
    23581 agcctgaaaa cgtcgttgct ggcttgcgac gagaccgttc ccttggaggc cggtgtcgat
    23641 gcccactcgt cgtcggctcc ggcgatcggc gcaccggagc aaggggccgg cctccggcat
    23701 cagtcccgct tggcggtggc cgaacaattg ctacgagtct atgaaaatgc ggtccggacg
    23761 caatcggcgg gaatagcgca ggcgaaacag gccctttggg agcgcgctaa tgcgcttgag
    23821 cgatctgaac aggcgttgag cgagcaaacc aggatcgtga actccgtcct gcggagtatg
    23881 ggcgacggag ttgtggtgac cgatgccgag gggcagctgg tcgttgccaa tccggcggtg
    23941 gaaagtattc tgggcttttc gccctttgac attcctctga cggcatgggc tgaacggtat
    24001 gagttttttc atgcggacac ggtgacaccc ttcgggcagg atgagtggcc gctggcgcgc
    24061 gcgctgcgag gcgaaaaaat cgactggctc gaagtctacc tacgggcctc gcagggtatg
    24121 ccgggccgct ggatcagcat caatgccagg ccgatcaagg atgaggccgg tgtgctctcc
    24181 gggaccgtgg tggtgtttca cgacattacg tggctcaagc gggcgcagga tgccttgttc
    24241 gaatcggaat atcgattccg gagtttggtg gaaggggcgc gcgatatcat cttcacgctc
    24301 tcggcggaca tcacgattac ctcgctcaac ccggcttttg agacggtgac caactggtcg
    24361 cgttcgcaat ggatggggga gccgctcgtt gccctgctgc atcccgatga cgcgggtttc
    24421 tgtcaggatg tgttgcaggc gattcttgaa cgcggggcgc cactgacctg ttcgatgcag
    24481 atcagcacga agcagggcgg gtacgtcacc ggagagtttg tcgggacccc gcaaatgcga
    24541 cagggccgtg tcatcggggt cttgggcatc attcgtgatg tgaccgaacg ccgccgaatc
    24601 gaggatgcgc ttcgcgtcag cgaggagcgg ttgcgctcca ttgtgcagtc gacgaaagac
    24661 gccatcgtat tggtcaatgc gctcatgaaa gtggcgttct ggaacaaggg cgctgaagcg
    24721 accttcggct attcggccga ggaaatcatc ggtcagcctg tgacgatgat cattcccgag
    24781 cagtaccatg aggaattgga acggaatgtt cagcgggttc gattgtttga acgggtgcag
    24841 ttgacgagca ggacgctgga actcctcggc cgtcgaaaag acggcggcga atttcccctc
    24901 gagctgagtg tgacctcctg gaaggggaaa tcggatctct tttttacgat catcatgcgc
    24961 gatatcagtg agcggcgatc ggcggaagag gaactggatc gcctgcatca tcacaaccaa
    25021 gtcgtgctca attcagccgg ggagggcatt tacgggatcg atcgggacgg gcggctgacg
    25081 ttcgtcaatc cggcggcggc gaaaatgttc ggctgggagg cggaggcatt gatcggccag
    25141 cccttttcta cgttggtcca ccgtcccgac ttccgtgagg gagcttccgg tgaacgactc
    25201 agtcccattg tggagaccat tcagggcggg aagattcggg aggaggcgga cagccggttc
    25261 tggcgaagag acggaaccag ttttccggtg gagtatgtga gtacgccgat tcgggagcgg
    25321 ggcgacatcg tcggggcggt cgtcgtattc aaggatacga cggatcggaa acgggctgag
    25381 gagcagttgc aggactcatt gcgtcgtctg cgcaaactgt cggggcggat ggaggggatt
    25441 cgagaagagg aacgcgggcg aattgcacgt gagttgcatg atgaattggg cgtcggcctg
    25501 acctgtctga agatcgatct gtcccgcctg ggtggattgc tgggggagcg gttggaaccc
    25561 cgggatcgcg caaaggtcga cgaaaaaatt cgaggcatga aggagcaggt ggatagtacc
    25621 atcacctctg tgcaacgtat cgtggcggag ttgcgccccg gcgtgctcga cgatcttggc
    25681 ctggtcgcgg ccatcgaatg gcagtgtcgc gatttccaac gccgcaccgg cgttgcctgt
    25741 cactgcaccg tcagtcatga agatttgcgg gtggacccgg aacatgccac cgcggttttt
    25801 cggatttgcc aggaggcgct gaccaacgtg acccgccatg ctcaggccac ggaggtacac
    25861 gtgcgcttgg aagaccaggg cggggggttg ctgttgcagg tcagcgacaa cggtcgtggc
    25921 attccatccg atcgccttgc ggatgccagg tcgttcggcc tgctggggat gcgggagcgc
    25981 gccgggctgc tcggcgggga tgtacagatc gagacgaagg agggcagcgg gacgacgatc
    26041 gcgctgcagc tgcctcggta agacggagga ccaggacatg atggagggca ctatgcgaac
    26101 aacagggacc gtgcggatac tcgtcgtcga tgatcaccct tcgtttcgtc gcggcgtgaa
    26161 ggatattctg gaagaaggat tcgagggcgc cagcatggct gagtgtggaa atgcgcagga
    26221 gatgctggac catgttcgcg agcagcccta cgacctcgtc gtcatggaca tcagtatgcc
    26281 gggacggagc gggccggaag tgttgaagga actgaagcaa ctggcgccga cgctgccggt
    26341 cttgatcttg agcatgcacc cggaagatca gtatgccatt cgtatgttta aggccggcgc
    26401 ggcagggtat ctcacgaagg cgagtgcgcc ggaagagctg gtccatgcag cgaaaaaagt
    26461 catgtcgggc gggcagtacg tcagtgcttc ggtgggagaa gccttggcgt tgacggtcag
    26521 gaccggggtc gacaagttgg cccatgaacg gctgtcggac cgggagtacg aagtgctctg
    26581 tctgattgct tccggacaga ccgtcagtga cattgccgaa agtattcatc tcagcgtgac
    26641 gacgatcagt acgtatcgag cgagaattct ggacaaaatg aatttgaaaa ataatgccga
    26701 gttgacgcgc tatgcgttgc agcatgggtt ggtcgtataa gggaatgggg tcggtcgcct
    26761 cgcccatgtc gttataacca tgacattgag gcactgtcta tttgtgacac aggaatcgca
    26821 cctctgccga tagcatcctc ctcttctcgg gcctatcctc atcttcatgc agaagccgtg
    26881 gatctccact acgacgacat tgcttgcggt ctttgaggct gcgcagggac agggatcggc
    26941 agagcgttct ggtattgatc gccgaaccgg gtcctttcgc ccgggttggc ccggcatcct
    27001 caccggattg accgccgtgg aagccgcgat gttacaagac ctcgcctggt ggggtcctca
    27061 aatggcgcgt cgattgacac aactcacagg cgttcgaacc acaacctaag ctgatccaga
    27121 cgagggtgaa ctgatggagc aaccgactgg tagcagcgga cagcgatccg ggaaacgacc
    27181 cacggtcgcc gtggtgtacg aagacgtcac gaccggctac aagtgtgccg attggtttgc
    27241 gctgcacggg taccaagcca ccatgacgtc ggcagtccat cacctggagc gcaacctgaa
    27301 ggaacttcgt ccggatgtga ttgtggtgag attgtcgtcc gctccgacgc cgctgcacag
    27361 taccgttccc agactcaaaa tgacctgccc gcaagtgccg attattgcga tggtggatca
    27421 atcaacggat gccctggatg gggtggctcc tgatctgacc aaggctttcg gtgctgatgt
    27481 ctttatgtgt gattcgctgg atccgccgtc gccgccctcg tagtacgcac gcctgacttg
    27541 ctcagacacc gcctagcata gacagtgcga cgatggtcag ccgtcccatc gtgccagtgt
    27601 ctcaccagct gaaaggagcc gaatcgtggc gaaacccaag gtattcgttc tcacagatga
    27661 tcaggcagcc gttgccgccg tgcgccgctc gatcgagagc gagtgtgagg tgttcgtggc
    27721 cgcatgggat cggacggcgg tggttcagat ccacgaacag gccccgctgg tcatgcttct
    27781 ggatttgggg tcctcgtccg atcccgctgt cgaggagcgt gtgcgggttc tgcaggaagt
    27841 gcgacaaacc gggcatgccg gaaaggtcat cgcctgcacg gaaaggacgg agcgccgcct
    27901 cgccgtccgc gcgatccagt atggagccta cgacgtgttg tctaaaccgc tggatatcac
    27961 ccttctggca caactcatcc ggcgggctgc gggagtggct gatctggagc gggaggcccg
    28021 aggcgtcatg gttggaggag gccctgaaga attcaacggt atgctcggca ccagtgccag
    28081 cattcaccgg atcttcgacg ccatacgaaa ggtttccacg aacgatgcac cgatcttgat
    28141 caccggggaa agcggcacag gcaaggagct gacggcgcgc gcgatccacg agagagggtt
    28201 acggcagcag ggaccgttca ttcccatcaa ttgcggggcg attccggaaa gtctgctgga
    28261 atcggagctc ttcgggtatg agcgtggcgc ctttacggga gcggtgaacc agaagaaagg
    28321 caaggttgaa tttgcccaag gggggacgct cttcctcgat gaagtgggcg aactcccgaa
    28381 tgccttgcag gtcaaattgt tgcggtttct gcaggatcac acgtttgagc gggtgggcgg
    28441 gcatcagccg attgaaatga acgtgcgaat catcgccgcg acgaatgtga atctgaagga
    28501 ggccattgaa aaaggaacgt tccgtgagga cctctactat cggctcgggg ttgtccatat
    28561 aaatgtgcct ccgcttcggg agcgcggtga ggatgtgtcg ttgatggcca tggcgttcct
    28621 gcgtcaggcg gcagcccatt acgataaaca tctggaaggt tttacccgcg aagcgctgga
    28681 ggcgatgcgc acctatgcct ggacggggaa tgtgcgtgaa ttgtccaaca gggtcggtcg
    28741 tgcggtggtg atggcagaag gcacgcacgt gactccggag gatctggata tcccgcatca
    28801 ggtggcgcga ccggacggca gctcgatttc gcttaaggtg aatcaacagc ggatcgagac
    28861 cgatctgatc atgaaggcct tcacgctgtc gcagggaaac ttgagtcgag ccgctcagga
    28921 attgggcatc agtcgttcga cgctgtatcg acggctgcgc cagtacggga tggaccagac
    28981 gcttgatgcg cggcgagcgc tcggggtgtt cccgcgcgcc tcgctgtcag agcattgatg
    29041 atgcggccgg cactgtgaga ttgatcgtgg ctggtgtgcc gtagcgatac aatcctgtca
    29101 ctctgagatc gatgcgagtg cataggtgtg taaaacggtt gaccgtcgta caggtatttg
    29161 gtacatacaa cagtctccga ttacctgctg atcatgctgt tctccggggt acggttcctc
    29221 acctcggctt gtggcccatc ctgtgacaat gtacgcgtga attggttgtt tcacgcatga
    29281 caggttgaga aatctacggg cgcagccttg tcaatcacgt gctgacggcg cgaaatggta
    29341 agtatgtttt cgagtgggag tcaatcgatg gtctccgatg caccagccct cttgagccgg
    29401 cgcggattgt tgcgcgccgc tacggccggg atggcgctgg ctgtggttcg cctggccctg
    29461 ccgtcggtga cgtgggcgtc acggttaccc gatggccggg tgagtttgta caatcttcac
    29521 accgatgaac ggttgtcggt gacgtaccgt gacgagtctg gcgcgtacga tcaggacgca
    29581 ctcaacgcgc tcaatcattt tctgcgctgc caccatacga acgaaaccac cacgatggac
    29641 gtgcagctca tcgaattcgt caatcttgtc caaaagcggg tcggcggccg tcgcgaagtg
    29701 catatcgtat ccggttaccg gtctccagaa tataacgagc agctcatcag aatgggcacg
    29761 cgtgcggcgc gtcacagcta ccatgtgtcg gggcaggccg tggatgtgca gattcccggt
    29821 gtgccgctga ggaccttgcg tgaggtggcg ttacggctcg gttgtggcgg ggtgggatat
    29881 tacccgcgtg gcaagtttgt ccatctcgat tcaggtccct tccgtcactg gtaacgacca
    29941 gttcttgccg ttcccttttc gttcacggcg cttctcagag catcaccgga tcgcctgctc
    30001 tcggtgtata ggtccgatca cctgccggca tgaggggcat tcctgctatt cccatgaccc
    30061 catgatcccg tcgtgatagg gtgcttctgg atgccgtctc tagcgcattg ccgtctgtta
    30121 tcccgaacac gagcggaggc tgcaggacat ggcggactga gatgggccga agcggaagtg
    30181 atggcaaccc atggggactg ccggtgaggg ggactgaatg atgaaggtat ctaagggcga
    30241 tatcgtttca gtcgagtaca ccattcgcct ggacgatgac cgtgtgattg agacaaccgt
    30301 tggtgaggcg ccgctgagct acacccatgg gcagaatgag atcttgcagg gactggaagc
    30361 cgggcttgat ggaatggagc gtggagcggc caaggtcatc accgttgagc cggtcgatgc
    30421 ctatggagag attcaccccg agggattctt tgaggtgcag cgagaacgtg tcccggcaga
    30481 ggcgcagcgg atcggaataa agttggaaac aacggctccg gatggccgag tggtattccc
    30541 ttacgtcgcc gagatcaggc cggacgttat tgtgctcgac ctcaaccacc cgcttgccgg
    30601 ccggacgttg cggttcgatg tgagagtggt ggatattctg cgtggtgagc gccagcttgt
    30661 cacggaggga gcgcgaggaa gcgagaaaga gacttaaccg accaggtccg ggttcatgcg
    30721 ggccatagac tggaccgatg tggctttgat ttcgttatac acaatggcgc ggccggcctg
    30781 acgaaggcga ctgaatatcc cttccaccac cacgtaatca ccttcccgca catctgcttt
    30841 tccaaggccc acaactttta ccgttcctgc ggtgtcctgc aggagaaatc cgaatgccgg
    30901 gtgaccgtcg cgattggtgg cgagctgaaa actgcttacg cgcccgctga cggtgacgac
    30961 ttgatgatca tagtgatccg ggtgggccag gagttctgtg acttcgacta aggctgcggc
    31021 atatgggaca ccgaccggca atgcccaagc catcatcgtc aacgtgccga gcagacacag
    31081 ggtgccaatg gcggtaggcc ggaaagaccg tctgtctcta cgttgcagca tgatagttgg
    31141 attatacaaa attcactcca ggctgagaag gaggacgtga aaaaactttt ctgaacaact
    31201 gggacgatgt gcaaccagcg tcgtaagcag gcgccggcgc gctgccagag cacgcgacta
    31261 tcgccgcaga tcgtcgtgca actcacccat aaagcttgtc agcacatgtt gtttcatctg
    31321 cccgacttct tccacgaccg gttgggcggc ttcatcgggc agtgccaagg attccttgct
    31381 gatcggggtc gtacgggtca ccatttcctg ctcgagcgca tccgggctgg tatcgatttc
    31441 ggacagtact gccatcaggc gttgcaggcc gaagagcgag cgggtatttt cagaatggac
    31501 ggcattcgaa ctggcgccat gaacgagtga cagccagaat ttcccttcga gatcctcccc
    31561 cagggtgccc cacgcgaagg ttcgaagctt ctccacgagc gcatcttcgg tccgctctaa
    31621 cccgtcatac tgcgcaatgg atcgctccac cggtcgttcc cccagcacgg cgagggtttc
    31681 tttccagagg tcaagcggcg cagtcgccgg aaggtccttt gccgacgacc gctggagaga
    31741 attttgaacg gcctgaaggt actggtggag atgtttgacc ttcctcgcgg cgagacttcc
    31801 ggccggaatg ccccagatat gcccgatttc gtgatcctgc aagggcgcgg cattaatcga
    31861 tttccattcc cgttccgaga gttcgaaccg tttccggtac ttgtccagca agcgcagctg
    31921 tgtctggatc tcaaggccga gccgggcttc ctggtacgct tcggcagaaa tgtcgtcttc
    31981 atcccatcga cggttcaaga gccggatcgc cggcttggga actgcagcac gggcctcttg
    32041 tttgagggca gtcacagctg cagcgtccac cttatactgt gtgagcaaca ggtccgaccc
    32101 ggcgcgaatc aacccctcgg cgagcgcgat catgcgcttc atggcttcca tctggagcca
    32161 gagccgctct cgtcgcaacc ggacccggcg gcgatattcg tcgcggcggc tcgtcacatc
    32221 gcgcagcgcg tcctgtttca caacttggtg gatcggtttc gaacccgcga cgcaacgtgg
    32281 atcgggccga tcagccgcga tgtgggtgat ttcctcctcc gtgatatcga aatgctgcaa
    32341 cagcagcagc ttgagcatga tgcggccctg aatcggggcc tgctcgatgg tcgaggcgat
    32401 cagttctggg gtgagcaaag cggtcatagt gtggcccctc gtgcgacgta aaaggttcgc
    32461 tcaggccgtg ccctgatggc tgttttcaag gtagaccgcg acatattcac gaatttcctg
    32521 gatgctgccg ctcgcaaaca attcccgagg aatctcaacc cgcaccagct tggcagggtc
    32581 gacgccccgg tcgatggcat attcttcgtc gatatacaga gtcacggaac cgtcaggaaa
    32641 gcggatggca tagaccgcgc tgttttcggc catggaggct gctccgtctg atgttgtgga
    32701 acggtatggc tattacaggt aacacagccc cagcccgagt gtcaaaacgc gtcgggggct
    32761 cgaatgggag tgctcagagt cggaagaccc gtctccgcgc gatcacgtaa ctcgtaaaga
    32821 cgaggagggc aatgaggagt gtgttccgga tccggccggg accaaaacag gccagattga
    32881 cgagggcatt ggtggtgccg taggcggcat agaccagata gatcaggagg gaccatttct
    32941 tgactcgtaa gaagccatac ccggccaggg tatggagaaa cggtgacagc gctttggtga
    33001 tgaacccggc cgagccgtcc agcctggtac aaaaaaacgg cagcaggtaa tcgggatttc
    33061 ttgcgatcaa gagggcatcg ttcccagcac tgaccagaaa gagcagtccg agaaaccgga
    33121 catcgtgtcc ctgcgcccag gcggtgcggg tgctgcgggc aaggtccgaa ccgctctcgg
    33181 acggcccgag tgcggcgtcg tagctctgca gggcccacca gggaagggcc aacaacataa
    33241 agaaaaagac ttcggcggct cgaccggaga cccgcccgag cgccaggctc aggaggatca
    33301 ggagaatggt gaccaggccg tagaagagtc ctgccccata tcgtccccga atgagttgtc
    33361 cgagcccggg gagtagggct gagtagatcg ccgcgcggcg atccgggata gcgtaagaag
    33421 gggcgtgggt ggacattggt cgagaacgcg tgagatcaga cggcgtgtcg ggatagaaac
    33481 ggcacggcct tcgatcaagg gatcgaaggc cgtgtccaca gactgctgcg caaccgttat
    33541 tagccgccca gggtgtccaa ggcttctttc aggctcttgc ggatgcaggt gaaaatcggg
    33601 gtgtcgcgca gggtgtaccg gctaccttcg gtttcccgga gcgccatgac gaggtccagg
    33661 aagtcttccg gcttgtcgct ctcgaaggcc acgacccatt cctgatcgtc gagaccgaag
    33721 gaataggtgg tgttgagctt gaccgagggg aagcgatggc cgacttcaat gtgctcgtcc
    33781 atcatgccct gccgcgccgc cttggtgagg aggaaccatt cgcgggtttt cacgaacgga
    33841 tagacgaaaa tgtacttggc tttgccgggg accacggtga gccgcttgct ttcctggttc
    33901 tcatgcgtgt ggtggtcgac ataaattgaa cgtttggtga tcgcgaggta cgaataggga
    33961 ttgtacagat acttgcccaa tccggatgcc atgatcttcg tgctcatttc ctggaacagt
    34021 tcaagctcgt agctgatgcg ccagagcatc agatcacagt cgcctcgaat acccaccgtg
    34081 gtgtaagcga tgacgagcac ttttccctga tactcttcga cggcacggag aaactcctgc
    34141 tttccgcgtg tgcgttcatc ttcaggaagc cgacgccatg cgggatcgat cttgtagaag
    34201 gtaaaattga cgtattgacg gcggggtgct tgttcggggc tggacatgac gaaccctcca
    34261 ggcaacttct tgataatgta aaagaataag agttagctct ttagcatttc gtctctgggg
    34321 ctgtcaacag agtccggggt gcgcggggct cgaattgaca ggcccacatc gatctgatac
    34381 tgcctgcata cgatgcgcgg cgttcgaaga ttgccggtcg gattctggct cgtgggaagc
    34441 ctcttcgtgg tcctgtgggg gagcaccggc tcctcgtatg ccatcgaggt gcagccgacc
    34501 accgaacaga ttcaggcgca ggtggagcgt ggaaagctgg ctgccggcca aggtgtctct
    34561 ccggatcagc tgcacgcctg gttcggtgcg accgaagacc ttgccccgcg cggtttcatc
    34621 atgacgaagc tgggaagcct gctggtgatg gccaatcacc ttgccctgcg cgcgttgtcg
    34681 ccgaccgaac aggatattgc ccaggtgctg gcaaacgagc atgtgctggt caatgtggtg
    34741 ttgtacggag accgtgtcaa ttttgccgcc gacagttaca ttgtgctgga gcaggccggt
    34801 cggacggtga agccctccac cgttcgattt gacgccaggg gcgatcgcac gacggcgtgg
    34861 ccgcagcagc ctgcctaccg tgcgaaggtg atcgcgcaat tcgcctacgc ggaactcgac
    34921 ccacgtgcga agacgacgct cacggtattt ccttcacgcg gaggccaggt cagcttcgaa
    34981 ctggacttct cgcggatcga atagtatcag tccctctcca tcgctctgcc catcgcaaag
    35041 gtgtatcccg catgagtaag accaaaacct ggacggctga aaccatcgcc gtcggatcgg
    35101 agttgctgct cggcggccgc ctcgacacga attcgctctt cattgccgat cgcctggccg
    35161 cctgtggaat tgagcttcga tataagacca cggtcggcga cgacctgtcc gacatcgtgg
    35221 ctgtgttgaa gaccgcgtta cggcgcgccc gcgtggtgat tctcaccggt gggttggggc
    35281 cgacgatcga cgacctcacg cgggaagccg tggcgcaagc gacgggccac cgcttgagcc
    35341 ggcgcaaggc tgccctggat gggatgattg cacggctggt cgaatggggg cgaactccga
    35401 ccaccacgca gtttcgccag gccatgattc ccgcccgtgc ggagattctc ttcaatccgg
    35461 tcggaacggc gccggggttt tcgcttgtct ggaagggcac gtttctcgcc gccttgcccg
    35521 gggtgccgcg tgagatggaa ccgatggtgc gtgatggcgt gttgccgcag ttgcaggcgt
    35581 ggatgcgcac acagaagagg ctcagcgcgt cgcccatgat ccggcgggtg ctccacacgt
    35641 ccggtgtgcc ggaatcaatc gtggaccagg ccctggtcgg gcttgttccc aaaggttgca
    35701 cgattcaact cggcatctat gcgtccccaa tggaagtcat ggtgtcgctg acgggaccgg
    35761 ctggagcgga gggggctgtc accatcgagc agttgttgaa ggaagccaag acgcggctcg
    35821 gtgacatcgt ttatggagaa gaagatgaga ccatggaatc ggtggtcggt cgattgctca
    35881 acgagcagca gttgacgctt gccgtggccg aatcctgtac cggtggactg atcggccatc
    35941 ggctgacgca ggtgccgggc gcatcgacat atgttgatcg agtggctgtc acctatagca
    36001 atcgtgccaa ggttgagatg ttgggtgtgc cgtcaggact gctcaaacgg catggggcgg
    36061 tcagcgcaga cgtggccgcc gcgatggcgc ggggcattcg tgagcggagc ggcgcctccg
    36121 tcggcgtgag cgtgaccggc atcgccgggc ccggtggcgc gaccgagagc aagccggtgg
    36181 gcctggtcta tgtggggctt gacggcgggc cgggcgaggc gatgacgaaa gagtttcgtt
    36241 ttcacggcgg tgaccgtacc gtgatcaagc aacggtcctc gcaggcggcg cttgatttgc
    36301 ttcgtcgctg gttgatcaag aaggggtgct aatggtccgg gcattcctgg ccatcgagtt
    36361 gccacaggaa ttgcgcacga cgcttgccac cattgagcag gacatgaagc gacaactcga
    36421 gcgaacggtc gatcgccatg tgagaatcag ctgggttcga ccggattcga tgcacctcac
    36481 cctcagattt ctcggcgaca cagccgacga atcgattgag ccgcttcggg tagccatcga
    36541 agagtccgtg tcagcgcatc aggcgattcc tattcctctc gaacgactgg gtacgttccc
    36601 gcgccggcag gcaccacggg tgctgtgggc gggaccctca gtgagttggg agcagggcct
    36661 tgaggcgcaa cggctcacaa gcctccatcg tgcggtggag gcttgttgcc gggcagcgaa
    36721 cttcgcgccg gaagagcggc cattcagtcc gcatttgacg ctcgcgcgga tcaaggaagg
    36781 ggagcggcag gtcggacagg cgctggctca gaacgggttg ttggagcggc cggtggcgat
    36841 cggcatgttg ccggtcgagg caatcgtcct catgaaaagt gaactgcgtc ccaccggctc
    36901 ggtctatacg aaactctggg aatgttcact agccccttct gcgacgtaag aaccgtaacg
    36961 ccctccaaac tttgccggaa gtggcatcat gctggggtcg ccccgccact attgctaggc
    37021 ccgccgtcag caggaacggt cgagtgcgtc gtttctgatt catggacagt ctgggcatgg
    37081 aagttttccg acgcccaagg cccggaatgg tatgatgatc ggtgataaac tacgagccca
    37141 tacgcaacgc aatctactac cggtccggcg ggagtccagc aagccgcctc aagcactaca
    37201 gcatgcagat ctagaaagct gatctattca atatgcctcc gttcaagctc gaagctccat
    37261 tcaaaccctg tggtgatcag gggcaagcca ttgagaagct gacatccgga atcctggccg
    37321 ggaagcagca tcaggcgttg ctcggggtca ccggctccgg caagaccttc acgatggcca
    37381 atgtcgtcga acgcgtccag aagccgaccc tcgtcctcgt gcataacaaa actctggccg
    37441 gccagctgta tcaggaattc aagcagttct tccctcacaa tgccgtcgag tacttcatca
    37501 gttattacga ttattaccaa cccgaagcct acatcccgca gagcgacacc tacattgcca
    37561 aggatgcctc gatcaacgat gcgatcgatc agatgcgcca tgcggcgacg acatccttgc
    37621 tgcagcgcaa cgatgtgctg atcgtgtcct cggtctcctg catctacggc cttggatcgc
    37681 cggaggtgta ccacgacatg ttggtctacc tggaagaggg gatggagacg cggcgtgaaa
    37741 agattctagc gaagctggtc gacattcagt atgcgcgcaa cgacgtggat tttcaccgcg
    37801 gcacctttcg cgcacgcggc gatgtcatcg agatttttcc agcctcgtcg gaagcgaaat
    37861 cggtgcgtat cgaattgttc ggcgatgtgg tggacgccat tcatgagatc gatccgttga
    37921 ccggtaaatc gctgggcaaa ttgccgaaga ttgccattta cccgaatacc cactatctca
    37981 ttgcgccgga ccggtatgaa cgggcgatca cgggcatcga agaagaattg gacgcccgcg
    38041 tggcggcatt caagaagaac ggtcagttgt tggaagcgca acggatcgag cagcgcacca
    38101 agttcgatct cgaaatgatc cgtgccatgg ggtattgcca cgggatcgag aactattcgc
    38161 gccacttaag cgggcgcgcg ccgggcgatc cgcccccgac ccttttggat tattttccca
    38221 aagacttcct gttgatcatc gacgaatcgc acgcgtcggt gccgcaggtc ggcggcatgt
    38281 acgaaggcga cttttcgcgc aaacggacgc tcgtggatta tggattccgg ctgccgtccg
    38341 ccgtcgataa tcggccgctg aagtttgccg agttcgagcg gatgctgaag caagtgatct
    38401 acgtctcggc gacgccgggt ccctatgagc tggagcatgc taagggtgag gtcatcgagc
    38461 agatcattcg cccgacggga ctgatggatc ccctgatcga tgtgcgatcg gcaaaggggc
    38521 aggtcgacaa tctgctggcc gaagtgcgga cggaggcggc gaagggaaat cgggtgctgg
    38581 tgacgaccct caccaagcga atggccgaag acctcacgga gtattatcac gacctcggcg
    38641 tgaaggtgcg ttatcttcac tccgacatta agacgctgga acgcgcggag attattcgcg
    38701 atctccggcg cggggtgttc gatgtgttgg tcgggatcaa cctgttacgg gaagggctcg
    38761 accttcccga ggtcagttta gtggcgatct tggacgcgga taaggagggc tacctccggt
    38821 cgcatcggtc gctgattcag acggcgggcc gcgccgctag aaatctggat gggcgcgtga
    38881 ttttctacgg tgatacgatc accgattcca tgcggcgcgc catggacgag acggcgcgcc
    38941 ggcgtcatat ccaggaagcc tataatgagg cccatggcat cacccccgag agcatcaaga
    39001 agcagattcc cctgctggac tatgcaacgg gtggcgggaa tgcggggcaa ctcgaactgg
    39061 cggcggagtc agtgggcgat tatgcaacga cgggcgacac ggagcagttg attcgccgtc
    39121 ttgaggttga gatgaaggcg gcagccaaga aattggagtt tgagcgggcg gcggagctac
    39181 ggaatcggat tcgaacgttg aggttgaagg cgttggaatt ggcgcaatag tgaaggcggc
    39241 tactgtccct cctgcgccgg aaattctgtt acatctgttt gtagagatag gccccgagaa
    39301 acatccccaa cacgctgagc aggctcacct tgatgctgaa gccgaacgtc agtttgagga
    39361 ggaccagatc gacggtgagt ggcgggttga ttccgggaga gaaattactg gcaaagatgt
    39421 tttgaatggc gccattcggg gccatgacgc gaagtatttc cccgaaaatc ccgcccagca
    39481 ttccgccgat caggataaag atcagcagca cgccgatcga ttttctcatc cgatatctct
    39541 cctagttccc gctgggcggg gtgcatgtgc cgactcgagg caccatatcg gaagcggaaa
    39601 atgatgtcaa caaaatgcat gccagacgcc tctgtggatg catgtggctg attgcaaagc
    39661 acctcttctg gagatcggtg ggagaataga gggcaccctc ggcgctcgag ggtgaagcag
    39721 ggagaacgcc cgacagtatt tctggtgcaa ctaagtttgg cgccgtgtgt cacgtgtatc
    39781 aacagtggtg cattggaacg gtccgagggc atcgcttgga atccctgccg aatatcttga
    39841 cttcacgcgc accccttcta tagactgccc gatgctattt tttgtgatgt ttttcgaggc
    39901 gatcggagct ctggctcgac cccggcttcg atggcctttt tgtttggtga ttcgtgcttg
    39961 atgcgttgag tgaaaagttc gaacgaatcc tcaagaagct tcgtgggcag ggtgtgctca
    40021 ccgaagacaa tattgctgag gcgttgaagg aagtccgcct cgcgttgctt gaggcggacg
    40081 tcaactttaa ggtcgtcaag gatttccttg accgcgttcg cgagaaggcg gtcgggcagg
    40141 aagttctgaa aagcctgacc cccgggcacc aggtcgtcaa agttgtctgg gacgagctcc
    40201 gcggcatgat gggcggtgag cggtccggta tcagtctcgc ctccagaccc ccgaccatta
    40261 ttatgatggt tgggttgcag ggagccggga aaacgaccac gtccggcaaa ctcgcccgct
    40321 tgtttaagag ccaggggaag cgcgtgttac tcgtggcggc cgacccgcgt cgtcctgccg
    40381 caggtgacca gttggccagc ctcgggcatg atcttggaat cgatgtgcat cgattcgatc
    40441 agatcgatgc ctcacgcgcc gatgtggtgc gtatctgcca gcgcggggtg gaacgcggtc
    40501 aagagcaggg ctacgatctg attgtgctcg ataccggcgg ccggttgcat gtcgatgatg
    40561 agttgatggc cgaactcgtg gcggtcaagc aggctgtgaa cccccacgaa gtgctgctgg
    40621 ttgccgatgc catgaccggc caggatgcgg tcaatatggc cggtcagttc gatcagcagg
    40681 tcggcctcac cggtgtcatc ctgacgaaag tcgagggcga cgcgcgtggt ggcgccgtgc
    40741 tgtcgatccg ggccgtgacc ggcaagccga ttaagtttct cggcatgggg gaaaagctcg
    40801 atgccctgga gccgttccat cctgaccgga tggcgtcacg gatcctcggg atgggtgatg
    40861 tgctttcgtt gatcgagaag gcgcaggaga cgttttcacg ggaagaggcc gaagcggcac
    40921 aaaagaagct gaccagcagc acctttacgc tggaggattt ccggtcacaa ctcgggcaga
    40981 tgaatcggtt ggggtcgttc gagcagattc tgggaatgct gcccggcggc caaaaattga
    41041 aagatcttgc gaacagcgga ttgcccgaaa aagaaatgaa gcgggttgcg gccatgatcg
    41101 actccatgac cacgcgtgag cggcgcgacc atacgctgat caacgggagc cggaagaaac
    41161 ggattgcgcg tggaagcgga acgagtgtgc aggaggtcaa ccgcctgatc aagcaattcc
    41221 tgcaggccag gaaaattgcc aaggtcatgt cgggtggcgc gggaggccgg cgtcagttgg
    41281 cccaaatgct ccgaggcatg tagcaggcca cgagtgtgtc gagtgtgcat cgattcgagg
    41341 tgaagaagag agtcctaacc gagcggacac agtctagggc tgaacgctca ttactctgag
    41401 gaggttctag tggcggttca tttacgattg gcacggacag ggcgacataa gcggccgatg
    41461 tatcgggtta ttgcggccga ttcacggaaa ccgcgcgacg ggcgcttttt ggagattctc
    41521 gggattttcg atccgctcaa gaacccggcg gtgccggtgt tgaagtcgga acgggtgctg
    41581 tcatggttgc ggcatggcgc ccagcccact gtgaccgttc ggactctgct gaagcggcat
    41641 ggggtttgga aagagttcga gaccgagaag tccgcaaagg cggagaagac cgaaaagacc
    41701 gagaagagcg caaagagcaa gaagtagccg gcatcacatg gcgacgctcg atcaagcaga
    41761 gctggtgacc attggaagga tcgagcgatc gttcggggtt cgaggcgagg cgcgcgtgcg
    41821 ttccctcaca gatgtgcctg gacggttcga gtcccttggg caggtcacga ttgtcgggcc
    41881 tgccgggaag acgctcgaca cccgtgtgac ccacgtgcgg ccaggcgggc cgacgtggat
    41941 tatggggttc gaggcgtttg cgacccctga gcaggtcgcc gagtttcgtg gagccttcat
    42001 tcaagttccc cgcggagact cgccggcgct gccggccgat caatactatc aatgtgattt
    42061 gatcgggatg gtcgtgcagg atgaagccgg aaccgtactg ggctgcttgg aagaggtcgt
    42121 gtctatgtcc ggcaaccaga actttctggt gcgtcacgac gggaaagaac tgctgattcc
    42181 tgcggcgaaa caagtcgtgg ttgcggtcga cgtagtgggg cgggtcatga cggtgcggct
    42241 gccggaaggg tttggagatc tgtaagatgc ggtgtgccgt tctgacattg tttccggata
    42301 tggtggctcc ggtgcttggc cagagtatcc tcaagcgcgc ccaggaaaag gggttgttgg
    42361 aggtttctgt gcagaacctg cgtgaccata ccttcgaccg gcataagacc gcggatgatg
    42421 tgccgtatgg cgggggagcc ggaatggtca tgaaggccga accggtgctt caggctgtcg
    42481 atgcgctgcg ggcccattat caggcttctc atccggagac gacgttgcgg gtgattattc
    42541 cgtctccgca agggcggccc tttacgcagg aactcgcaca gtccctggcg caggatgaac
    42601 gggtagtggt gtttgtgtgt gggcactatg aggggatgga tgagcgcgtc aggctggcgc
    42661 tgcaccccga agaaatctcc gtcggcgatt atgtgttgac cggaggggag ctgccggcgt
    42721 tggtgatgat cgatgcggcg gcgcgcctgg tccctggcgt gttgggcgat gcggcgtcgg
    42781 cggccgagga atcgtttacc gagggattgt tggagtatcc gcactacacc agaccggcgg
    42841 aggtgcgcgg gatggcggtt ccggaggtac tggtatcggg tcaccatgag gcgatccggt
    42901 tgtggcgccg gaaggaagcg ttgcggaaca cctatctgaa gcggccggat ctcctgcggg
    42961 atcgcgagct tggatcggaa gatcgacggt tgttaagcga agtgatgcaa gagagtcttg
    43021 tacaggtacc tgttcgttga gaggaggaga aggactatga atcggctaga acggatccaa
    43081 cgatccttaa ccaagaagac gacgcccaag ttcgagatcg gggatactgt tcgtgtccac
    43141 gtgaaggtgg tcgagggtga aaaagagcgt atccaggtgt atgaggggac ggtcatcgcc
    43201 aggaaaggca cgctcaatag cgaaaccttc actgtgcgca agctgtccta caacgtcggc
    43261 gttgagcgga cgtttcccat ccactcgccc aatgtggcca aggttgatgt cgtccgtcag
    43321 ggtcgtgttc gccgggccaa gctctattat ttgcgcacca agaagggcaa gtttgccaag
    43381 gtggaagatc gcgagtttaa ggtcgagagc aaggcgcaaa ccgcagccaa gcgtgaggcg
    43441 gcagcagcag ccacggttgc caaggcctga tcgatcgttt gtgctgtgtt ccgcttgaga
    43501 ccaggacaga ggcgcacgtt tggctggttc ttcgaacccg acgacagggg gccccaccga
    43561 ggagtttgag gtggaggctc ggtgtcgcgg gtaccggcat atagccgggc tcgatgaggc
    43621 cggacgtggg cctctcgccg gtcctgtcgt agcggctgcc gtgctgttgc cgcgtcgatg
    43681 ccgcctgccc ggactcaacg attcgaagca ggtcggcgag tcagaccgag ttcgattgtt
    43741 cgccgaaatc gttcgccggg caaccggcgt cggcatcgga gctgcgacgg aggcggagat
    43801 cgatcggttg aatatccttc aagcaacccg gttggcgatg cggcgtgctc tgcaggcatt
    43861 accgcttcaa ccggactttt tgttgttgga tgcggtcact ctttccgggc tctcagttcc
    43921 tcaacgttct atcatcaagg gcgatggcct ctcctgctcc attgcggccg cctcgatcat
    43981 cgccaaggtg acgcgcgacc gtttgatggt tgagtaccac cgttggtatc cccaatataa
    44041 ttttgccgag cataaagggt acgggactcc ggagcatctc cgtctgctcc gggagcacgg
    44101 gccctgtgcg attcatcgcc ggagttttgc gcctgtgcag cagcactgtg tcggcgatgc
    44161 tcgaaaattg tgttcctctg tcgaccagtc tgagagcccg ctggaccgat gcgcgaccga
    44221 cgccgattgg taggggatga aggagagggg cgagccgagg cctatctgcg ccgacagggg
    44281 tttcgaatcc ttggacggaa tgtgcgctct ccattgggtg aactcgacct ggttgccgat
    44341 gaccaggggg tgctggtgtt catcgaggtg aaacggcgac ggtctgggac ctacggtggt
    44401 gcgattgaag cggtggatgc gcgcaaacgt gccaaattga ttcagcttgc ggcgcagtat
    44461 ctggcgcaac accggattca tgatcgtgcg tgccgatttg atgtcgtctt gatacaggat
    44521 gattccggga cgactgaacc cgtgcagcat attgcgaatg cattcgacgt cgcgggggat
    44581 gatctgcgat ggtaggatgg gaatcgcgct cctgattcat ccggtcactg tgtgtcgtgc
    44641 cgggcggcgg gggcctgaga ggtcactccg gtccggcggc tgctcttgtc accatccatg
    44701 attcaacttt ttcacgtctc aaaatattac gaccgccgtt tggcgctgtc cgatgtgacc
    44761 cttgaaatcg agaagggcga gttcgtgctc ttgatgggac cgagcggcgc cggaaaatcg
    44821 accttgttga aattgctgat cggcgcagag cgccctgagg aagggcagat cctggtgcag
    44881 ggccgtagtt tgtcgaaatt gcgatcgtct gaaattccct tactgcgtcg aaagatcggt
    44941 gtggtgctgc aggatttccg cctgctgcca aagaaaaccg tattcgacaa tgtgtcgttg
    45001 cccttgttgg tgcagggggc ctcgactgtc gaaatcaggc ggaaagtgac ggacgcatta
    45061 cggtcggttg ggctggatca caagaaggat ctctttcccc ccggcctctc taccggtgag
    45121 cagcagcgga tttgcatcgc tcgcgcgatc gtcaacgggc cgatcatgct gttggccgat
    45181 gaaccgacgg ggaatctgga tccggagctg accagcgaaa tcatcgaact gttcaagtcg
    45241 atcaacgccc ggggcacgac catcatcgtg gcgacgcacg atcccaatgt cttggcccag
    45301 gtcaatcgcc gggtgatcac attggaccag ggcaaggtgg tctcgcagga acaggtgtgc
    45361 tcgtgaggcg tgtgttgtac ctgctgcggg aagcggtcgc caatgtgttg accaatcgaa
    45421 cgaccacggt ggtcgcggtg gccaccacgg ccttcacgtt tgcctgcgtc ggcgtgtttt
    45481 tgttgttgta tgtcaatttg aggaccatgg cctcgtcgct tgagcaggac atccaggtta
    45541 tggtgtacct gcaggacgac ctgacggagc agacgagaaa cgaaatcgaa ctgcaactga
    45601 aggccgatcg tgccgtcggg tcgttgacct tcgtgtccaa ggaacgggcg ttggctgatt
    45661 tccagagtca gtttccggcc gagtcccatc tgctgcaggg gctcgggcag aacccgctgc
    45721 cggcctcatt tgtcgtgacg cttgcggtgg agtcccgttc atcggatgcg atgcggcggt
    45781 gggccaatcg cacccagtcg atcccgggag ttgcgcaggt gcaatacaat caggaatggg
    45841 tcgaggcctt ggccggcatt gtccggtata tcgaggtggc tgccatcatt gtcggcctca
    45901 ttttatcggc ggcgtcggtc acgatcatcg cgaacacaat ccgcctggcg ctgtattcac
    45961 gccgggagga aattgaaatt ctggggttga tcggggcgag tacgaccttt atccgtgtgc
    46021 cctatttgct cgaaggtgcg gcgctcgggt tgctcggcag tgctctctcg ctggtaatcc
    46081 tcaaagccgg gtttgagttg ttccgccatg aaatccggtc tgccagccgg tttctgggcg
    46141 tggacgcgct gctgacattt ttccccttcc aaatgtgtgt ggcgcttgtc tgtgtggggc
    46201 tctttctcgg ttgtgccggc agttttctct cgctgctccg attcggagag ggacgggcat
    46261 gaagtgggcg cgatggggat gtgttgtggt ggctgctcat ctggtcttgt ggactgtccc
    46321 tgcctgggcg gagcgcaagg actcgtacgc ggacaagatc gaacaagaaa agaaaacgct
    46381 ggaaaagttg cgcggcacga ttgtcgagaa acgtaagaag gcggacgagg cggaaaagaa
    46441 acgcgagtcg gtcctgcagg gattgcagtc cttggacgaa cgtctggttc gttaccggca
    46501 ggaacaccag gacgtcgtga agaagctcaa aaagaaggat gtggaaatcg agcagatgag
    46561 cgtgcagctg agccggctgt ccgaacgcat cgatgaacgg caggatgcca tcgctgctcg
    46621 cttgcgggtt caatatgtcg aggggcggtt ttggcacctg agaacgctct tggcggccag
    46681 ttcttcccga gactttcagc ggcggtttcg atatctctcg gccgtgacgc agcgagagta
    46741 tgagatcatg gaaacctacc ggaaagatgc cgagcggatc gccgaggtcg agcggagtcg
    46801 agaagaggcg cgccagggga tcctggccta caaggccagc accgaggaaa agctggctca
    46861 gatccagggg ctgaaaaaac agaaacgggt atatctcgct aaaatcactc aagaaaagga
    46921 atcgcatgat cgggctgtag aggaactgga gcggtctgcc aaccgggtcg acagcttgct
    46981 gaaggaactg gaaaccagac gtcgggcagc gctggcgact cgtccaccct ccgcgacggg
    47041 aggccttcgc gcactacgtg ggacgctcct ctggccgacc gaagggcaag tggtgtccta
    47101 cttcggccgg cagaagcatc cgaccttcaa tacctatatc cagcgaaaag ggattgagat
    47161 tcgcgctgcg gaagggagta atatacggtc ggtgcttgcc ggccaggttg tctacgcgga
    47221 ctggttgaaa ggctacggac tcgttataat catggatcat gcaaacggag tcttttcgtt
    47281 gtatgcccat gcgtccaaaa tattgacgtc ggtcggggcc cggatcgaag ccggagaagc
    47341 gatcggtgaa acgggcgaca cgggcatgac gggtgagaat acgctctatt ttgagttgcg
    47401 cgagggagcg gaaccggtgg atcccctcgt gtggctgtcg aagcgatagg cctcggaagg
    47461 aaggaaacct acgttatgga acagcggcga agccgacggt ggttatatct tttgctgatg
    47521 gtgacggtag ccctgggtat cggcattgtg ctggaaaagg gattggagcg gaccggccac
    47581 gcctccgaga cctatgaaga gctgcggacc ttctccgagg tcttgaccca ggtgcagaag
    47641 cactacgtcg acgatacaaa ggtgaaagac ctcgtacagg gtgcgattcg cgggatgctc
    47701 tccacgttgg atccgcactc ggcctatatg acacctgaga tgtacaaaga aatgcaggtc
    47761 gaaacgaaag gcgaattcgg tggggtcggg attcagatcg gtgtgaagga gaaccgcctc
    47821 gccgtcatct ccccgatcga aggcacgcct gcgcatcgag ccgggatcaa ggccggtgat
    47881 ttcattacca aggtgaacga cgaacccaca aaggatttga cgctgatgga tgcggtgcag
    47941 aaaatgcgcg gccccaaagg caccaaggtg aacctcacga tccagcggga cggcacggcg
    48001 gacccgctgg cgttttcatt ggtgcgggac accatcaaga tcgaaagcgt gaagttcaag
    48061 gtgctcgaca ataccattgg gtatgtgcgc ttgacgcagt tccaggaagc cacgggacgg
    48121 gacctcagcc gggcgcttaa gcagttcaag gagcagaagg ttcaggggac gattctcgac
    48181 ttgcgcaaca atcccggtgg cctgttgacg gcggccgtcg atgtgtccga acagtttgtg
    48241 gggaacggga agctgatcgt ctataccaaa ggtcgtgagg ggaagaaaga tgagtggttc
    48301 tccaagacga aggagacgct ggaagattcg ccgatgatca tcctggtgaa cgaagggtcg
    48361 gctagcgcgt cggagattgt ggccggtgcg ttgcaggatt ggggacgggc cgtcattgtc
    48421 gggaccacgt cgttcggcaa gggatcggtc cagacgattc tgccgctggg tgacgggtcg
    48481 ggactccggc tcacgactgc caagtactac acgccgaagg ggcgctcgat tcagtccacc
    48541 ggcatcacgc cggacattgt ggtcaaactg caaacgcaga ccgtcgcaaa ggccggcgag
    48601 aaagacggaa aagagtctga accgaagacg gccaaagcgc ctgcagccgg aaaggatcca
    48661 tcgccttcgg ccaagccggg tgaggagcct gcgcacaaga atggcgccgt gtcggctggg
    48721 gatggggccg gggatatttc gctggatgat gacgtgcagc ttcaaaaggc cgttgagctg
    48781 ttgaagacct ggaagatctt taaggagctg cggccggcgg cgtagtcggc acggttccga
    48841 tccgttcgcg gatggcggcg agcagcgtgt cttccgaccc ggaaaaacct ggttgtgttc
    48901 gtaagagatg ggacgcaacc aggctttcca ggtcggccaa tgtgcggatg ttcagccgga
    48961 aatataagta actcaccagg gcatcggaga ggcccggcaa cgaagcccag ccggccactt
    49021 cctccggcaa cggagtcttc agttcttcat aggcgcgaat cgttcctgtc gccagaaact
    49081 ctccaatctt cactgccaag tcccgcccga ttccggggat ctcctgaagc tgatggcgcg
    49141 cggctagatc agccaggtct cccgtaaccg tcaggatcga atcagccgct ttccgatacg
    49201 cgcggacgcg ataaggattg gcacgttggc ttgccaacag gttggccatc gcacgaaaga
    49261 tctgcgcaag ttgatgggtg cggtcgtcca tagtcgtgtg cccggcggtc tttatctaca
    49321 ttgtgcgagt atcgaatccc aaacggcaag gccttaccgg gcttggttga caaccggttt
    49381 tcccgtttca taggatgatc tgaagaattg aggtgcgcag tgatcattca cgtgaatggt
    49441 gaatctcgcg gaatcggcga tggggagacg gttgcagcgc tgttgcacga gttggagatc
    49501 cgcgcggatc gcgtcgccgt cgaattgaat ttggagattc tggatcgcaa ggattttgaa
    49561 acccgcggac tccgcgaggg ggatcgggtg gagatattga gctttattgg tggcggagcg
    49621 aggtaaccac atttatggat acgatcaacg atcgcttagt gattgccggc cgggaattta
    49681 agtctcgcct ctgggtgggg accggtaagt ataaagactt tgtcgagact aagaaggcta
    49741 tcgaggcgtc cggtgcggac gtggtgacgg tggcggttcg tcgcgtgaac atcacggatc
    49801 ggtccaagga aaatctcctg gattatctcg atccgaaaaa atacatcatt ttgccgaaca
    49861 ccgccggttg ttacacggtg gaagatgccg tccggtatgc ccggttggct cgcgcggccg
    49921 gcgtgtccga tctggtcaag ctggaagtcc tgggtgatga aaagactctt ttcccggaca
    49981 ccgcgggatt gatcgaggcg gccaagatcc tcatcaaaga aggcttcatc gtcctgccct
    50041 atacgaacga tgacccgatc gttgccaaga agctggtgga tatcggctgt cctgcggtga
    50101 tgccgctggc tgctccgatc ggctcggggc tcgggatccg taatccatac aatctcaaga
    50161 tcatcatgga aacggtcaag gtgcccatca tcgtggacgc cggagtcgga acggcatccg
    50221 atgccgcgct ggcgatggag tacggagcgg atgcggtgct gatgaacacc gctattgccg
    50281 gcgcgcagga ccccatcgcg atggccgagg ccatgaagta tggtgtctgg gccgggcgac
    50341 tggcctacaa ggccgggcgc attccgcgca agctctatgc gacagccagt agcccgatcg
    50401 aaggcatgct ctaatagcca gagaaccgtc tttggtcggt gtcggcgagc cgggctgagt
    50461 gcgtgtgtga gtggtgaacc ggtcctgctc tcccggcatc tcacattcaa ctggcatcat
    50521 tcagcatcca gaatatttcc cgaatgccct ccgtcgattt ccgtctgtat ttagtgactg
    50581 atcgtcaaca gaccgccgga cgtccgctgg tgtcggtcgt cgggcgggcg gtgagtgccg
    50641 gtgtgcgcgc ggtgcagctg cgtgaacgtg atcttccgat ccgagagctg ctctctttgg
    50701 cgctcgacct gcagcgcgag ttgtcggaca tgcagttgtt catcaatgac cgtgtggatc
    50761 tggcggtggc actggggtgc cggggggcgc atttgcgcga gagcagtttg ccggccccgg
    50821 tggtgcgaac gctgttgcag ccctcgcagc tgttgggtct gtccgtccat tccatccagg
    50881 atgcggtggc ggcagaacgg cacggcgcgg attttgtggt gttgggtccg atttacgaca
    50941 cgccttcgaa gcgggagtat ggggcgccgc ttggcctcca ggtactcgaa caggccgcgc
    51001 gcgcggtgaa cattccaatt tttgccatcg gagggatgac tgcctcgcgt acacgtgagg
    51061 tgcggcaggc gggagcattc ggtgtggcgg tgttgtcctc gatcttgagt gcgtcgaatg
    51121 tcgaagtagc gaccgagaaa tttctttcgg cgatcgagcg cgaatcatga agaggattgc
    51181 tgcagagggc gtctcacgca tcgacacacg tcacgatgta tcgccgtctg ctgctgttga
    51241 ccaccccata ggcgggtgat agaatccgaa acagtgttga tgaccgttac tgacagggat
    51301 tgtaagcgat ccaacgagtg gggcgatggc cgaatcaaaa gggagttcag gccgactccg
    51361 gtccttccgt gattccgtca agcgcctgac caagtcactt tctgccggag atggagtcgt
    51421 gacacacaag aacgacgaac aggcccgcga ggtagaaaaa cttcgcatcc aaatccagtc
    51481 catggaggag gagattcggc ggctctatca gtcacgctac cagctcgaac aaacgacgaa
    51541 gcagaacgaa aagctcgtcg ccactctcca ggaagccaaa tctcaaatcg aaacattgcg
    51601 ggctgaagtg gagaagttga ccgctccgcc ctcgacctac ggcattttct ccagcctcga
    51661 taccgatggc acggggaatg tctacgtatc cggtcgtaag atgaaggtca gtcttcatcc
    51721 ctccatcaaa gcgaagtctc tccgcaaagg gcaggaggtc atcctcaacg aagcgctcaa
    51781 tgtcatcgag gtgcgtgggt tcgatgtgca aggggaagtc gtccgtttga aggatgtgct
    51841 ggaggggaat cgggcgctgg tgacgctgca cttcgatgaa gaaaaagtcg cggagcttgg
    51901 cgagccattg ctgagtgagc gcctcagcgt cggcgatcat ctgttatacg atccgcggtc
    51961 cggttgcgtc atcgaaaagc tgccgaaatc ggaagccgag gagctggtgc tcgaagaagt
    52021 ccccgatgtg gactacgaac atatcggcgg actccagaaa gaaatcgagc aggtgcgtga
    52081 tgcggtggag ttgcctttcc tgtatccgca catttttgcg aactacaaac tcagcgcccc
    52141 gaagggcgtg ttgctctatg gcccgccggg ttgcggcaag acgttgatcg ccaaggccgt
    52201 ggccagttcg atcgccaaaa aactggggca tctcaaggac aaacaactgc gcagctactt
    52261 cctccatgtg aaggggcctg aactgttgaa taagtatgtc ggcgagtcgg agcggcaggt
    52321 gcgcgaggtg ttcaaaaaag ccaaggaacg cgccgacgac ggtcatcccg tgattgtgtt
    52381 tttcgacgag atggacgcac tgttccgcac ccgcggcacc ggcatctcct cggacattga
    52441 atcgaccatc gtgccgcagt tcctctcgga gatcgacggc gtagagcgtc ttaccaatgt
    52501 catcgtcatc ggggcaagca accgacagga cttgatcgat ccggccgtgc tccgcgcggg
    52561 ccgtctcgat gtgaaggtga aggtcggtcg tccggacgcc acggcggccc gggatatttt
    52621 ctccaagtac gtctcgaccg atttgccgtt tgccgaggac gatctgaagc ggcatggcgg
    52681 ggatgcccgg gcacttgtgg attccctcat gaacatgacc gtcgatgcca tgtatgcgac
    52741 gacggacgaa aacaagttca ttgaagtgac gtatgcgaac ggcgagaagg aagtgttgta
    52801 cttcaaggat ttcgccagcg gcgcgttgat cgagggcatc gtgtcgcgtg ccaagaaatt
    52861 cgccgtgaag cgggcgattg cgcaagaagg agccggtctg cgggccgagg atctgatccg
    52921 cgcgatccga gaggagttca aagagcacga agacttgccc aacaccacga atccggatga
    52981 ttgggcgaag gtcgccggca agaaaggcga gaagattgtc cacctccgga cgatcagcgg
    53041 cgggcctgca gaggcgcgcc aaatcgaaac ggtcaacacc ggtcactacc tgtagcgcga
    53101 tatttcatga gcgacaatcc tacgcacaat ccgtcccgtg tcatcggcac ggagaccgaa
    53161 ttcggtatcg ccagtcgtga cccgaatgcg gccgatccgg tggccaactc cattcacctc
    53221 atcggctact acccgaatct tccggctccg catgcggtgt gggactacga gaacgaaaat
    53281 cccctgctgg atgcgcgcgg gtttgaggtc gatggtgagc gggaacggcc gggacctgat
    53341 tacaatcggc aactcaataa agtcctggcc aacggtggtc gtctctatgt cgatggcgcg
    53401 catcctgaat attccacgcc tgagtgtctc aatgcgaggg aagtcgtggc gttcgagcgc
    53461 gtcggtgaac gcattgtcgc gcaggccctg gaagggatta ccaaggcgcg cgggcgtgac
    53521 cagttcgtgt tgtataagaa taattcagac ggtaagggca acagctatgg gtaccacgag
    53581 aattatctcg tgtcgcgggc ggtgccattt gaacgcatca cccaggtcct cacgccgttc
    53641 tttgtgacgc ggtcgattta tgccgggtcc ggcaaggtcg gcgcggaaaa ccagaccagc
    53701 cccgtcgact atcagatttc gcagcgggcg gattttttcg aaacgctcgt ggatctgaac
    53761 acgatggttc ggcggccgat catcaatacg cgcgacgaac cgcattctga ttcggcgaag
    53821 taccggcggc tccatgtgat cgtcggagac gccaatatgg ccgaggtatc gacctatctg
    53881 aaggtcggga ccctctcgat tgtgttggag ttgctcgagg ccggcgccga ccttcctcga
    53941 atcagcctgg cagatccggt gaatgccatc aagcaggtct cccgggatgt gcaggtgaag
    54001 gagagtttga agctcagtga cgggagctca tccactgcga tcgccgtgca gcgagcgtac
    54061 ctcaaggccg cccagagcta ttatgcctgc catgaactca accaggtgac gaaggacgtg
    54121 ctggtgcggt gggaagatgt gcttgatcgc ctggaacggg accctcggtc attggtgcgt
    54181 gagttggatt gggtcgcgaa gcggtacctg atcgaattct atatggaacg caaatcctgt
    54241 ggctgggacg atccgcgcgt ccgtctgatg gatttccaat accatgatgt ccgtcccgac
    54301 aagggcctgt attataccct ggaacggaat catcgaattg agcgggtggt attggatcat
    54361 gagattgcgc gggcggaatt caatccacct gttgggacca gggcctattt caggggccag
    54421 tgcatcaaga agtacccgac tgtcgtctac ggggcaagtt ggacgtccgt gctgttcgat
    54481 actggacaaa acaagatcaa gagaatcccc ttgatggatc ccctgcgggg taccgaatcg
    54541 ctcacggggg aactcctggc gcaagcggag acggcgtccg cgctgctcgc taaactttcg
    54601 acctgactgt gaccattgaa tgaaacattc cttcttcttt tcacatcacg aggggtccag
    54661 cttcttcgac ttcctcaccc tgcatcatcc tgagttaaga cccggtttga gtgagatcgc
    54721 catccagcga gccgcgcttc ccgtggattt cggccgaccc ggaacgatcc cggtgccgca
    54781 tgggacgacg gttctcgcgt tgaaatatcg ggatggggcc attattgccg gtgatcgacg
    54841 cgcgaccgag ggatttcaga ttgccgaccg gcggattgaa aaggtctttc gcatcgacga
    54901 ctattccgcg atggcgatcg ccggggctgc ggggccgtgc atcgagatgg ccaaattgtt
    54961 tcagacggag cttgagcatt acgagaagct cgaaggcgtg cagctgtcct gtgaggggaa
    55021 ggccaacaag ctggggcaga tggtgaaggc caacctcccg atggtatttc agggactcgt
    55081 cgtcatgccg ctttacgtcg gatacgatct caagcggaac gaagggcgca tttttaagta
    55141 cgacattaca gggggacgct acgaggagtc cgaccaccat tccatcggct ccggcggcaa
    55201 ggatgcccgc aacacgatgc gtgaacatta ccgatccggc ctccaggaag aagaggcgct
    55261 gcgggtcggg ctgttggccc tctataatgc ggcggatgag gatgtgggta ccggtgggcc
    55321 tgatttagtc cgcggtattt atccgacggc gaaaatcgtc agtagcgccg gtatcactga
    55381 tgtgcccgaa gatcgggtgc gtgccttgtt cgaaaccatg atcgcagagc gaaggagatc
    55441 ctagccgatg ccgttgccct actacgtctc gcctgaacaa atgatgcagg acaaggcgga
    55501 gtatgccaaa aaaggcattg ccaaaggacg ctccatcatt gcgcttgaat atatcgacgg
    55561 catcctgctc gcagcggaca atccaagctc ctcattgcat aaagtgtcag aggtctacga
    55621 ccggatcgcg tttgccggag ccggaaaata tagcgagttc gagcatctcc ggaaggccgg
    55681 cattcgccat gccgatctga cagggtacat gtacagccgg gaagatgtga gcgcgcggac
    55741 tttgtccaat gcctattcgc agagtctggg gacggccttc agcacggacg tcaagccgct
    55801 ggaagtcgaa atccttgtcg tgcaggtcgg tgtgaacggt caggcgaatg agatcttccg
    55861 tatttcattc gacggcagca tcgtggatga aaaaaatctc gcggtgatcg ggggacggtc
    55921 ggaagccgtt cagcaatatt tgcgggaaca tgcgacaccg cagccaccca cgttgaagga
    55981 tgggctccgg cgttgccttg ccgccctgga gcaggtcgcc actcaaaaga tcccttccga
    56041 aaatcttgaa gtcgccgtgc tggaccggaa ccgccttggg cggaagttca aacggttgag
    56101 cagtgccgac atctcccagc tcttcgcctg acatcccctc cacgtaactg cgtgcttatc
    56161 cgcgttccgg tcggtgagac gcttttcatt gccgggcatc tgttgaagcc tcacccgccg
    56221 cggtgtatcc tgagagaagc ggattcgagg aaacgtctct ccatgcagca acgtatcttc
    56281 ggtttagaaa acgagtacgg cctaattttc tctccgaacg ggaagattta tcttccgatg
    56341 gagaaagtgc tgggctacat cttcgaaggc ctcattccaa acagctggcc gtcgaatgca
    56401 ttcctggtca acggcgcgcg cttttatcag gataccggct gccatccgga gtactccacg
    56461 ccggagtgcg acaatatcct cgatctcgtc gtccacgata aagcgggcga gcgcttgctg
    56521 gaagcctgtt tgccggcggc tgaagagcgc ttgcgcgaag aggggttgtc cggagaaatt
    56581 tatattttta agaacaatac cgattcgctc ggcaatacct acgggtgcca tgaaaattat
    56641 ctcatgcgcc gggacgtgga tttctggaaa gtcaccgagc agctcattcc gttttttgtg
    56701 acccggcaga tctattccgg cgccggtaag gtcctgaaag tctcgggtaa accgcagtac
    56761 ttcatctcgc agcgcgccca gcatattcac gaaaagacct cctcctcgac gacctcgtcc
    56821 cggagtatca tcaacacccg tgacgagccc cacgccgatg cggagaagta tcggcggctg
    56881 cacatcattg tgggcgactc caatatgtcg gagtatgcga cgtatctgaa ggtcggcacc
    56941 gccacgttgg tgctctcgat gatcgaagcc gggtactcgg tcagcgggat ggaactcgag
    57001 gatcctgtga aagccatccg tgaaatttcc cgcgatccga ccctcaacaa gaaggtcaag
    57061 ctggatgacg gccgccagat gactgccatc gagatccagc gggtctacgt caagcgtgcg
    57121 aaagagtttc tgtcggagca ggaacacgat ccggtgctgg acgacgtctg ggagaagtgg
    57181 gtgtcggtgt tggatcgtct ggaagaggat ccgatgcaac tcgtgcacga ggtcgattgg
    57241 gtgatgaaaa agcatctgat tcagtcgtac accgacaaga aggattgcgg ctgggatgac
    57301 ccccgggtgt tcctgttgga tttgcaattt catgacgtga aacgcacgcg ggggctgtat
    57361 tatctgatgg agaatcgagg gatggccgcg cgggtcgtgg aggaggaagc ggtgcaacgg
    57421 gcgatgtccg tgccgcctca gaccacgcgc gcaaaagtcc gcggcgactt tatccggttc
    57481 gcgcgggcca agaaccggtc gtacacggtc gactggacgt atttgaagtt gaacgggtac
    57541 tgggaagaga ccattctctg tatggatcct ttcagcgcgg tgaaccggcg ggtcgaagaa
    57601 ttgttatcgc aggtgtccgg cgcgcgacta tatcgatgac agaggggcac gggcagagac
    57661 gagacaagac catgtcgttc ctggatcgtt ttctcatcat ttcggtcgtg ctgctgacga
    57721 gtgtgtttcc cgtcgaactg tttccgaaca cggggccggc gcctcgtggc ggggacgggc
    57781 aattcagcgg caagcagggc caggtgatcc tcatcaacgt gtcggacatc aaggatgcgg
    57841 ccagcgtcaa aggtcagttt cttgggcgca aggtgacgct ctttccagac ccttctgccg
    57901 atggcggtgc cggttatgtc ggattgctgg ggatcgacct acaggatgag ccgggtgcgc
    57961 atgagctcac gatcgatgcc caactgggtg agcagacgcg ccatttttcc tatcaggtgt
    58021 tggtgctgaa ggagaagttc gccgtcgagc atctgaagtt gccgaaagac aaagtcgatc
    58081 tcgacgagaa agcggctgcg cgctggaagg ccgagcagga gcaggttcgc aaggcgttgg
    58141 ctgaagagtc tgccatgcgg ctctggcagg ctgggttcat cgaaccggtc catggtaagc
    58201 ggacagggat ttttggcagc gtccgcatca tgaacggtca ggccagaaac ccgcacaacg
    58261 gagaagatat cggcgcgccg atgggcaccg acgtgatggc cagcaatgac ggtgtcgtcc
    58321 ggctcattgt ggaccatatc ttttcaggcc gcggcatctt tgtggaccat ggtctcggcc
    58381 tgtactcgat gtactttcac ctctccgacg tgctggtgaa agaaggtgat ttgatcaagg
    58441 ccgggcagat catcggcaaa gtcggcgcca ccggtcgcgc cacgggtccg catctccatt
    58501 ggggcatgaa ggtcaacggg gcgcgcgtga atccctacgc tctgctcgaa ttgccctttc
    58561 cccagaaccc cgctggtgca cggcctgtgt tggcctcccc gacgcgtccc gcacagggcg
    58621 aatcaagcgc caccgcagtc ggcggtgata cgcgataacg cgactcgctg ctgacccgcc
    58681 gtcccatcca gccggcggat caattctcag gatccgtgag ccgtttccct caggtctgat
    58741 tcgacaacgt tggatcaagc gcggcgttca ctgcctttat cagcgagtcg ggggtatagg
    58801 gcttgggcag aaatatggtc cgtgcatcaa gctgttccag catcgatccc tcgcggtctg
    58861 aatagccgga ggtgaggatg atgttgatct ccggttgcag cgtccggagt tcggcggcga
    58921 gttgtatccc ccccatgccc ggcatcacca tatcggtaat cacgagtgag atttttcctc
    58981 cccgctggcg aaccagctgg agcgcttcct cgccggttcg cgttggcagg accgaatgat
    59041 gctgtccgac cagcaccgtc tgtgccaggc ctcgaaccat gtcattgtct tcgaccagga
    59101 gaatagtttc cgactgttca atgatgctcc tggattgagc cacagggcgc ggcgattcgg
    59161 aatcctcttc gatgcacggc agataaatcg taaatgtggt gccacgtccg ggctcgctct
    59221 caatcccaac cgtccctccg ctctgccgga cgattccgta gaccgtggac aatccgaggc
    59281 cggtaccttt gcctagctcc ttcgtggtga aaaacggctc aaacacatga gcgaccgtgt
    59341 cggcatccat cccgcatcct gtgtcccgga cggcgagcgt gacgtacggg cccggggtga
    59401 ggggatcagg ccagagcgga tcggggtgag cgatcgagat cgatttcgtc tccaggtcca
    59461 ggcggcctcc ctgcggcatc gcatcgcgcg cattgaccac caaattcatc acgacctgct
    59521 cgatttgacc gggatccgcc ttgattcgtc caagcgcagg atccaaggtg agggcgagtt
    59581 gaatgtcttc cccgatgaca cgtttcatga gctcgacaat gtcggtcacg cggtcattca
    59641 ggttgagcgg ttggcgttcc accatctggt gacgtgtgaa ggccaacaac tgccgggtga
    59701 gggcagtggc tttattgccg gcttccctga tttgtgccat cccgcgctgc gtccgggtgg
    59761 tttgtcccgg ctcatccgtc aagagctcag cccagctatt gataatcgtc aaaagattgt
    59821 taaagtcgtg tgcaattcct ccggccatgc gtcccagggc ctccattttt tgcgcctgac
    59881 gaagctggcg ctcgagatcc tttcggtgaa gaaattgagt gatcatggcc cccaactcgg
    59941 acgtccgttc caatcgagat gagctgggcc gcagccgttc gtaggtgaga aactccatga
    60001 tccccaggat ctcttttccc gtgtgaatgg gaaacgcgat ccctccgtgg aggtcggcct
    60061 cttgcgcagc gtgaaggatc gggcagtcgg tctcgctgag aatatccggg cagagagtgg
    60121 catcccctgt cttccagacc tggcccgcca aaccgcctcc ctgccgcagg gcaaggcttc
    60181 gatagattcg ggcgaagttc tcggcaggga ggcccggctg gtgccacagg gcattgcagc
    60241 gcaaggtctt gtcttgtgaa tgcaccaacc acaatgcacc cagcgtccag ccttgaagct
    60301 cgcacatggt gcgaagaatt ttggggaccg cttcactgag agaggtggat tcttcaagcg
    60361 cgaggccgac cgcgagttga acggcatgca tcgtttcggc ttgccggcgc tcggtgatgt
    60421 cggccttggc gccgatcatg cggtaggggt ggccctcgtg gtcccggagc acatacccgt
    60481 gatcaatgac ataggcatag gtgccgtcgg ctcgacgaaa tcgatattcg gctgtccaca
    60541 ggttctggtc cgactggagc acttgagcga ggttggtgag gatcgcggcc tggtcctcgg
    60601 gatgaatctg ttcggcccat ggaatgtggc ctcgccgaag cttctcaggg gaggaaccat
    60661 ataccgattg gtgcgcctcg ttgaaccatg tcacgccgga ggtgatttcc cagtcccaga
    60721 taccgtcgcg ggtggcgagg caggtcagat gaaaccgctc ctcgctcttc cgtaaggctt
    60781 gctcgacgcg gtgccggccc atggcataac ggatggcgcg tcggagtgcg tctcccgtca
    60841 gccggccctt cacgagatag tcctgcgcgc cgtgcaggag ggatttctca gccaccacgt
    60901 cgttatcgag ccctgtcaac acgatgaccg gcgcgtcatg tgcttgagcc cgcacccgat
    60961 cgagcgtctc caggccctgg ctgtccggca gcgacagatc gaccaggacg gcatccaccg
    61021 ggcattccgc cagttttttt atgcccgttt ggagtctgtc cgcccagttg agcgtgacag
    61081 cgtccgtcga ctgctcgcag agcgcctgtt ggatcagctc ggcatcatct tcgttgtcct
    61141 cgatcagcaa cacatgcatg tgttacctct tcagcgtcgg gactcgcgag accagcgtcc
    61201 aatagagttc gaactctttc acgaccgaca ggaaccggct gaatgtgaca ggctttcgaa
    61261 tgtacgagca ggcgccctgt tcaaaagacc gcacgatatc ctcctcgcgc tcactgacgg
    61321 tcagcatgac gaccgggagc ggacgaaagc gggcgtcgga cttaatttca atcagcacct
    61381 cgagtccggt tttcttcggc atgttgatat cgaggagcac caggccgggg aacggacatt
    61441 ggctgaaagg accttcaccc cgcaaatagg ccagcgcttc ttcgccgtcc cgcacgacgg
    61501 ccactcgatt ggccatgttc acggtctcga aggcttccct gatcaacaag atgtcgtctt
    61561 cgttgtcttc tgcgatgaga atatcaaagg gtcgtatggt catcgttgta ccttcctcgg
    61621 actagactgg ttgacgccga atgtgatgaa aaattccgat ccgccgcctt cgcggggttc
    61681 cacccaggca cggccaccgt gtcgttcggc cacttgccgg acgatggcca gtcctgcgcc
    61741 ggtcccctct atctcgcgtc cgaccgcccg tcggaagagt tcgaatatcc gatcgcgttg
    61801 ctcaggagcc actcccgggc cacggtcgcg cacgaccagg ccgatgagtt cgtttccttc
    61861 aagatccatc ccggaatgtg gcgcaatctc aatgtcagga gcttgcccgg cggatgcaaa
    61921 cttgagcgca ttcgcgacca gattatagat accctggatg gcccaggtgg tattgacccg
    61981 caggcgggga aggggggagc gaatcgtgat tctggcgtcg gtttctttga tgcgcttctc
    62041 cagccgacgt aggacttcgt gcaccagctg ttccgcgtcg atgtcgtcga cgggcggatc
    62101 catgcgctgt gcacgtgaga ggtccaggat gtctgtgagc agttgatcga ggcgctgcgt
    62161 ggcccgaacg atgcgccgca gaaaatcctg acccttctca tcgagccggt ttgcatagcg
    62221 ttcctggatg aggacagaaa aactttcaat ggcccgcagc ggttctttca gatcgtggga
    62281 agtcacgtac aagagcgttt cgaggtcctt gttcttcttg aggatcatgg tttcggactg
    62341 gcgcagctga gtgatatccc ggttggtttc caggacgagg ctccggccgt tggtcttgag
    62401 caatccccag cggctggcca cgagcacgtc ccgcccgtcc ttcgtgcgat ggtgaatctc
    62461 gccggtccat tctccggtct gttccaattc ggcctggatc gtgccgaggg gcgccggcag
    62521 ccggctctgc agcagacgat tgctgctgtg ccccaaggct tccgatcgag tatagccgta
    62581 cagctgttcg cacccacggt tccactccac gatgccctgg tcgatctccc aggcgaaaat
    62641 cggttcgtaa gacaattcga tgagcctgcg ttgctgggtc atcatctcct cggtctgctt
    62701 tcgcgccgtg atgtcacgag cgatcgtcga gacggcgttg acgagaccgt tggcatccat
    62761 gatgggggaa accgttaagg agacctggat acaactgccg cctttccgtc tccgaatagt
    62821 ttcgtactgt tgcacccgtg ttcctgtttt gatcagttcg atcagggtgg tttcttcgag
    62881 ttgacggtcc gatggaatga gaaggaaaat cgattggccg atgatttcct cgccggagta
    62941 cccgaacatc agctcggcgc tcttgttcca ggtcagaatg cgtccgtcga gtgatttgct
    63001 gatgacggcg tcggaagagg attcgacaat ggcggcgagc ctggcctgca tggactccgc
    63061 ctgtttgcgg tcatggatct ccgtgaaggt ccccaaccat ctgaggaggc ggccggcgtt
    63121 gtcccgaagc ggtgacgcgt gcgcatgaaa ccaacgatag gtgccgtccg cccttcgaag
    63181 acgaaactcg ctgtcgaacg gctgtgcacg tgcgagtgcc tcttgccact gctcgatcgc
    63241 catctggcgg tcttcggaat gcacttgccg agtccatccg tgaccgagct gccccgccgc
    63301 cggcattccc gtataactca tccattgcgg actcagatag tcgcactctc cttccgggag
    63361 gcaggtccag atcggttggg ggaggcattc caccaactcc cgaaatcgtc cctcgctgtc
    63421 ggccaacgcg gtttggatac gattgctctg agccagctcc tgtgccaatc gtcggttggt
    63481 ctgctcaagc gcggcggtac gctctttgac gagcacctcg agcgtctgat gactttgacg
    63541 cagggcgaca tcggcctttc ggcgttccgt gatatcgacg agggtggagc ggctggcgat
    63601 gaaccgaccg tcgggttcct taatggccgt ggcgctcagc agaaccggca gcagagagcc
    63661 gtctttgcgc cgaatctcga actccagatc ccgcacgatt cctcgctcga tgaaacgtgg
    63721 gaactgttga tgaaatagtt cgacagactc gggggtgaga aggtcttgga agcgcattat
    63781 tccgatgacc tcgtctttgc gatagcccag ccagtcgagt tcagtttggt tgatgccgac
    63841 gaacatgcct tgcgagttca gtgaatggta tccgcagggc gcatggtcgt agaggtcctg
    63901 tacgtcctgc gcgtttttca gcagcaggct ctctttcaat tgtaaggcct tgtgttccgc
    63961 gtccagtgcg gccgtttgaa tcaggcgttc ggttgcccgc cggtactgga cgatcagatg
    64021 ggcgatgact gcaaacatgc ccaatgccga ggcgcgattc aggagaggca tccatgaggg
    64081 catgctggtc atagggccgg acagggcgag acacagcaat gtgaggatgg cgcacactcc
    64141 cgccgtgagg tagggaatcc atgcgacagg agcctggtac gtgaggatca acgggatgag
    64201 gtagagaacc cagacaggaa caccgagggg cgttaccacg tcgaggaaga agatgattcc
    64261 gatcgaggcg acaatgaggc taaggatggt ccgcccacga gggaaaggat cactcgtgct
    64321 tgacttgagc gcctcgttct cggctatagc cacatcgctc ctttacagga gtcctgaatc
    64381 tcataaggag ccagtattac aagaggctat cgcaatcgac gctactatgc acctaattac
    64441 ctcatgcgag tagagtcaat ccgacctagg cctttaccta ggtgcggtat ctgcgtataa
    64501 gaagactgtc ggattctcgg aggatgaccg tacgggcacc ggccggaagt cttcccggaa
    64561 ccaggacggg gcctagaaat tcgtttccat gctcgacggg gtcttattca gcacggttgc
    64621 cgcggcgcgg gagagggccg cgtcttgatc gtggtcgagg gcgtagatgc ggaattgcac
    64681 gaggcgggtg gggagcagat ccaaaaactc ttccagcggt tcagttgaaa cacccttcgt
    64741 gccgggatcg aagacgagca gggggatgcc gcgcgtatcc ttgggatccg ggcgcggatc
    64801 gagcggggcc atgtcgacgc gaaacggcaa tgtctgcaat tttgccggca acgctttctg
    64861 aatctgctgc tggaattgct ggtggctggg gaaatccatg cctcgctcga tacccttctc
    64921 cttgagcacc gtgctgtagg ccattttcca cttcacgtcg cgccccagga tatgcgccca
    64981 ctcctgatgt aaacgacgca ggctgctttg gccggctttc ttccatcgcc tgacatcctc
    65041 caacaacgac cagtcggtca aggtcaggta ttcatccatg tgcttggctg ggttggacgg
    65101 gaacaacagc ttcatcgtgt ccccgaagat gtcgcgcaga tgaatgtcga tggcgcgggt
    65161 ggtgcggtga tagtacacgt tcgaatacag atacattctg gtgttcagaa acatctgcag
    65221 ggccggcagc ccggtcttat gaatggtgaa gcccttgtcg gtgacgatgg tgtagtgaat
    65281 cagacgggtc aggtctaccg gccccaccgc gacaccgcac atgtacgcat cgcgcaagac
    65341 gtaatcaagg ttgtcggctg tgtagctgcc tgaaatcact ggctggagca tgttgatcca
    65401 gcgcggaatg cgggagttat ccttgccctt ctccttcagg atcacatgcg caatgtgatc
    65461 cggattcagc tcttctccgc gatcgaactg gcccgacgga ctccggcgga ttttcttgat
    65521 gagggggccc aggtgctcgc gcacgatgat ctgaccgagc ttttcatgcg agagaccgaa
    65581 accatgcaaa aagttatcgt cgaagaaatg gcagaatgga ccatgtccga tgtcatgcac
    65641 gagagccgtg atgcgtagaa actcctcgac caaagccgcc gagggaacgt cgggcaccgc
    65701 cttcttcaag aagggataga gatggcgcgc aaaacggccc gctacatgca tggtgccgag
    65761 cgagtgcacg aatcgcgtat gttccgctga ggggtagacc cagcgcgcgc tttggagttg
    65821 atagatgtag cgcagtcgct gcacccatgg agaatcgatc agatcttttt ccgtgcgttc
    65881 cgagggatcc ggcgtcgcgt acggcaccgt aaacgacacg tattcgtgga tgggatcggc
    65941 gatgagggct gatccgtcat agggagcggg gggatgtgcg gttggtagtt tcatgcggtc
    66001 ggcggatgaa tgatcttagc gcagacctga tgaagggggc aacggttgat gcccgtccgc
    66061 cgggtttgct ctccggtact tggagagcaa gagataggcg agcggatagg ctaacaggcc
    66121 gcctgcgata ctcaggacca tcccgccgac gaagaacggc caggcgtagg gcatgacctg
    66181 atgataaatc cccatgaagg acagatcgct ccagtcgaag gacggaaagt catgcactcc
    66241 caatagggtg gcgccggtcc aataggtgcc tccgaggatg gggatcagcg tccagggatt
    66301 gttgaggaat gcgccggcca acagcgccac cacgttcagc ccgaaggccc aggcgcagaa
    66361 ccccaccatg accatatgaa gaccataggc cggagaaaag ccgatgaagc tgccgatggc
    66421 aaaggccagc gccgtccgat gcggtgtttc atcgagatgg agcacttggc gaaacaacga
    66481 tcgtacgtct gccatcgatg ttcttgtccc ggatatcgct aagagggccg cagaaaatgt
    66541 tcataactgg tgaccggcag cggttgcgcg gttcctgctg cggttcgcaa gcgcagaccg
    66601 agccgcttgg gagtgatcga gccgatacag gtgaagcaaa agccggtctt ctccgccagc
    66661 tgttcgaatt gctcttgctt gcggcgcgga acggtgaaca ggagctcgta gtcctcgccg
    66721 ccctgtaacc cgatctgctg cggatcacgg ccatgcgcca gggcgtaggc ccgacaggcc
    66781 ggagaaatcg ggagtgagcc ggcgagcact tccgctccga cgccgctctc ttcgcagagg
    66841 tgtcggaggt caccggtcag accatcggac aaatcgatcg ccgcaccggc gagtccatgt
    66901 ttcaccaacc attggccttc tgcaatgcgg gcggacggcc ggtggtgacg cgcgagcaga
    66961 aagcgggtgt gtgtgggacg cagtttcgcc gctccggcgc gggccgtggc cgtcaggagg
    67021 tccagccctg cgcgggagtc gccgagtgtg ccggtgacgt acagccggtc gccgacgcgg
    67081 gcgccgctgc gcaggagagc gcgtcgaggt tgtacggttc cggtgagtgt gatgctgata
    67141 aacaggccgt acctggaggc ggacgtgtct ccgccgacga ggcagacccg atagggatcg
    67201 gcggcctgca tcatgccgcg atagagtcgt tgaatctgtt gcgtcgagca ggtcggcgga
    67261 attgcaatgg ccacgagcat gaatcggggg gtgccgccca tggcggcgat atcgctcaag
    67321 ttggcgatgg ccgcccggta tccgatgtct tcataggagg aggtggcagg gttgaagtgc
    67381 acaccttcag ccaggaggtc cgtggtgatg agagtccatt cgtgggacga ggttttgagg
    67441 atggccgtgt catcgccgat ccccttgaac acctgccggt cgggtcgggc ggcctgcgct
    67501 tgcagcagcc ggatcaggcc gaactcgcta ccgacagaac tggtccggcg ggatcggcct
    67561 gacggcatat tagagaatca acgatggcga agccgtcgga aggcggaggc ttttccgggg
    67621 cgagccggtt gttcgcttgg atgtggtcgc gcgcgccgat ttcttgaccg gagccttcgg
    67681 ggccgatgcg cgcggtttgc gggacgatga tcgtcgcagg gccgccttga tgacatcgtc
    67741 gaccgtatcg acaaagatca actcgatgcc cttgaggata tgtttaggaa tttcctccag
    67801 gtccttcttg ttgcgcttcg gaaggacgac ggtcgcgagt ttcgcccgtt tggccgcgag
    67861 gattttttcc ttcaagcctc cgatcggcaa gacgcgcccg cgcagggtga tttctccggt
    67921 catcgcgaga tcgcgccgta cggggatctg ggccagcgcc gaagcgatgg ccgtggccat
    67981 ggtaatgccg gcagacggtc cgtctttcgg tgtggcgccg gccggcacgt gaatgtgaat
    68041 gtcgttcttg gcgaagatgt cggggctgat gcccagggtc ttctctcgcg agcggacata
    68101 gctcagggcg gcctgcgcgg attctttcat gacgtcgccg agatgcccgg tgagggtgag
    68161 ttgccccttg cccttcatga cggttgcttc gatatacagc acgtcgccgc cgctttccgt
    68221 ccaagcaagg cctgtggcga cgccgatttc gtctttttca agctccgcct cgggaacgaa
    68281 cttcgccaca cccagaaact tgttcaaatt ggtctggtcg atggtgtgac actcggattt
    68341 gccctcggcc accttcttgg ccactttccg catgaggttg gcgatttccc gttcgaggtt
    68401 ccggactccg gcctcgcgcg tgtagtgcga gatgacgtgg cggatggtcg cctcgttcac
    68461 gcggatatgt ttttcggtaa tgccgtgctc gttcatctgt ctgggaatta agtacttctg
    68521 cgcgatcccg agtttctctt cttccgtata tcccgggatg tcgatgactt ccatgcgatc
    68581 ccgtaacgcg ggcagaatcg gatccatgag gttggccgtc gtgacgaaca tgacttcggt
    68641 caggtcgaag ggcaccccga gatagtgatc ggtgaaggtg ctgttttgtt cgggatccag
    68701 cacttccagc agcgcggccg aaggatcgcc gcggaaatcc atgccgactt tgtcgacctc
    68761 gtcgagcatg aacacgggat tgttcgtgcc ggcttgtttc atgccctgga taatccgtcc
    68821 cggcagtgcg ccgacgtagg tcctgcgatg gccgcggatt tcggcctcgt cgcggactcc
    68881 gcccagactg atccggacga actcgcggcc gagcgcgcgg gcgatggatt tgccgagcga
    68941 ggttttgccg acgccgggcg gtcccacgaa acagaggatg ggccccttca ttttctcttt
    69001 gagcttgcgc acggcgaggt attcgacgat gcgttctttc accttttcca agtcgtagtg
    69061 gtcctcgttc aggaccttca tcgcggcttt caggtcgaga ttgtctttcg attttttatt
    69121 ccacgggagc tcgaccatcc attccagata ggtccggacc gtggctgact ccgccgtgtc
    69181 cggatgcatt ttttcgagcc gcttgagctg cttctcggtt tccttgagca ccttctcggg
    69241 catcttggcg tctttgatgc gcttgcggaa ttccgccact tcctccgccc gttcgtccaa
    69301 ctcgccgagt tctttctgga tggctttcaa ctgctcgcgc aggaagtatt cgcgctgggt
    69361 cttgtccatc tcccctttgg cttgcgcctg gatcttctgc tgcatggaga ggacatcgat
    69421 ttccttggag agaatttcgc tgatctggcg cagccgctgg atcgggtcca cgatttccag
    69481 gacggcctgc gtgatgtcga ccttcagtcc gagattggag gccaccatgt cggcgagccg
    69541 gccggggtct tccagatttt cgatgaccac catcacgtcg ggaatcagca ccttgccgag
    69601 gctgacgatc ttctcgatct gctccttgac cgtgcgcatg acggcctcgg tctccagtgt
    69661 ggatccggcc tgcttcgtct cgacgagttt ctcgatgcga accgaataat aggggtcgtt
    69721 ctggatgtat tcttcgatcc gtcccttggc gagtccctgg actaaaatct tgatccgctc
    69781 gtcgggcagc ttgagcatcc gcatgatgat gcccacggtg ccgacggcgt gaatgtcgtc
    69841 cggttgcggg ttttccacat ccagggattt ctgggtggcg aggaacaaca tgcggttgcc
    69901 cgcgagcgcg gcttcgatcg ccttgatcga catctcgcgt ccgacaaaca gcgggagcac
    69961 catgtaagga aacacgacga tatctcggac aggcagcagg ggcagctggt ccggcggttc
    70021 gaggttctgg ttgcttgaga agtcttgttc tactgcttca gccatgaggc tccagtctgc
    70081 tgctgtaggg tctcagtgat ggtgacgtgc tgtggcacgg tctgggtgac ggtaatccgt
    70141 catgcccaag gctagttggt ggtgcgtgtt cgaccttggc cggatcggct tgggccgcgg
    70201 cgacgcacgt cggcggcggt cggtcggagt cgttgttgta ggaactcgcc gaacgcaggt
    70261 cccaaagatg ggttcttgag cgccaattcc acggtggcaa tgaggaatcc gagtttgtcg
    70321 cccgcatcgt gccgctgtcc ctcgatttcg tgggcgaaca tcggggtgtg cttgacgagt
    70381 tgccgcaggg cgtcggtcag ctgaatttct ccgttcttac ctggaggcgt cttgcggagg
    70441 atcgggaaga tctccggcgg caagacatac cggccgatga cggcgagatt cgacggcgca
    70501 tcggcgggcg cgggtttctc gaccaggtca tcgacccggt gcagcccctt gcccaggcgt
    70561 cgggcagaga tgatgccgta tcggctcacc tcttggggtg gaacttcctg tacgcctaag
    70621 acggcgcctt tgcgttgctt gtaaatatga atgagttgcg cgaggccggg gaccgccgcg
    70681 tcgatgattt catcgcccaa aatgacggcg aacggctcat cgccgatcag gtgctgggcg
    70741 cagagcacgg catgcccgag gcccatggct tccggctggc ggacgtaaca gaagttcgcc
    70801 agattcgaaa tgtgccgcat ctggttgagc acctgcccct tgccgctgcc cttgagattt
    70861 tcttccagtt ccagggagcg gtcgaagtgg tcttcgatcg cgcgcttgcc gcggcccgtg
    70921 atgacgatga tgtcttcgat accggatgcg acggcttcct ccacgacata ctgaatcagc
    70981 ggtttgtcga ccagcggcag catctctttg ggagaggctt tggtggcggg aaggaagcga
    71041 gtgcccagtc cggcagcggg aataatagct ttacgtacat ctgttctcgt cataggggga
    71101 tagtatgtga tggggtaagt gaagtcaaga tttcctccgg tcttttcggc gcggcgcgtg
    71161 cgtcgttgcg cggggaaact cctttacatt gaaaaatgtg gtcaatataa tccgagagtt
    71221 tttctgcaca tgtgcggtgg tgctgcaacg aaagcggcgc atcggtgatg gtaacgaaaa
    71281 cagttcgtga gtgcctgcgc aaggccggtc ggcccgacgc acgagacgcg acacggaacg
    71341 cggtggtttc ctatctcgaa cttcaggaca accagtgctt gcattccgaa gtggtacgga
    71401 aggattgcgg tgaccaagtc gggaggcagg cggtgggtgc cgattcttat tctcgcgctg
    71461 gtggcgctct acgcgttcgg tgtaggggcg cctctgtacg cccaggacgg cgtgccgaca
    71521 gtcacgtcgg tcaccattcg tgggcagaag cgcattgaac tccaggcgat cgaggggcgt
    71581 ctcacgctca aggccaatga tcgttttacg gccgacgcgc tccgcgagca ggtcaagatc
    71641 ctgtacggca ccggctactt cgaagacgta caggtggaga cagaaccggc cgcgggaggt
    71701 gtgtccgtcg gttttgtcgt tcgggagaaa cccttcatca cggagatcgt gttcgacggc
    71761 aacgaggaat tgagcgacga caagctcaag gaaaaggtca cgatcaagag ccagacgttc
    71821 ctcgaccagc agcaggccaa agagagcgcg gaaaaaatcc ggctcgccta tcaagaggac
    71881 ggctactaca actgtcaggt cattcccgtc atccaggcgg tggatgagga ccggaaacgg
    71941 ttgacctact tcatcaagga aggcaccaag gccaaagtcc tccatatcaa tttcgagggc
    72001 atgcgggcgg tgaccaagga agaaatcttt aaggtgacgg cgacccggga atggattccc
    72061 tggtatggac tcatcacgca gttgaaggtg ccgtcgctgc tgtccgatgc cggcgtgctg
    72121 aaacgcgagg aactgggcaa cgatatcgaa cgcatgcggg aagtctattt gaacaagggc
    72181 tatctgaacg tgcagatcag ccagccgacg ctggaactga gcgaggacaa gaagtggttc
    72241 gaaatcaact attcgatcgt tgagggcgag ccgttcatcg tgcaggaggt cgggttccgc
    72301 gggaatacgg tgttcgagga tcatgaattg cgcgaagggc tgaacatcag accgggcgaa
    72361 atttttcagc gggccaagat ccggggagaa atcacccgca tcacggatct gtacggcgcg
    72421 aaggggtatg tgttcgccga cgtcgtgccc aacgtgacgc ccgacaacaa ttcgcgcacg
    72481 gccacgattc tcctcaatgt gaaagaggga gaaatgatgc ggatccgcga gatccacgtc
    72541 aacggcaacg acaagacccg cgacaacgtg gtgcgccgcg aactgcggtt ggatgagcag
    72601 gatgtggtcg acaccctggc gttgaaacgc agttttcagc gattgaacaa tctcaacttt
    72661 ttcgagaccg tcgaaattct gccgcaacag gtcgacgtcg ataaagtcga tctcaacgtg
    72721 aaggtgaagg agaagccgac cggccagttc agcatcggcg gtggattcag cacattggat
    72781 aagctggtgg cgatcgcgga tatcaccgaa ggaaatctcg gcgggaacgg ctggttgggt
    72841 cgtatccgcg ggcaattggg ccagcaacga tcgttgggat tggtgacgtt ccgcaatccc
    72901 tatgtgaacg actcgtacaa tgccctgcag ctggatattt atcggtcgat gaccaactac
    72961 atttcctact tcgagtcgaa gtcgggtgtc agcgcgacct ggagccgatg gttgtcggag
    73021 tatgtgaacg ggagcatcag tctgttcggc gaacaactca aatacagcga ccccgaagca
    73081 gggctgtgcc cggatttcat tccgctcatt tgccgccagc tcgggacgca atcgtcgacg
    73141 ggttttcgta cctcgatttt tcgcgacacg cgcgactatt acctggatcc gcgcaccggc
    73201 tggcgcttca gtctcggggt cgactacgga acgcctgcgc tgggcggcag caatcacttt
    73261 ttcaagtatt atgcggacgt gatcaagtat acgccgctga tctacgatac gcggttctcg
    73321 gtgcacgttc ggtacggctc gacggagggg atcgggggcc ggcccattcc cctcaccgag
    73381 cggttcttcg tcggcggtat caacaccatg cgcggattcg tgttcgggcg ggcggggcct
    73441 gtgacgccga acggatccct cttgggttcc gcgaaggaac tcattttcaa caacgatttt
    73501 atcttcacca tttcgtccga agcgaagctg aacggcgtgt tcttcttcga ttacggaaat
    73561 ggattcgacg acaacgaacc ggtccagttc aacaaactgc ggagcgcagc cgggttcgaa
    73621 gccaggtgga tttctccgtt cggccccttg cgggcggcct acggaatcaa cctggatccc
    73681 aggccgacag agcgaatggg tgtgttcgag tttacgatcg ggtcgttgtt ctaaggcagt
    73741 ccgtgtcggt gcgtgagaaa ggaatcgccg gaatgggtgg accagtgggc cgcagtggat
    73801 cgaggggagc cggtcgggcc gggttgtgcc tggcgcttgt gaccgtggcg ttggttgtcg
    73861 gcgggtgccg gtccggtcac tcgacggtgc cgacttcgac acgtatcggt gtggtcgacc
    73921 cccagcgcgt gttgagcgac accaatgccg ggaggaaggc aaaggacatg ctggcgtctt
    73981 ttgccaagaa ccggcaggcc ttgatcgagt tggaggaaaa agagttgcgg cggatggaag
    74041 aagacttcat gaagcagggc agcgtgttga gcgccaacgc caagcgcgag cgggaggaac
    74101 aattccgtcg tcgcatgtcg gagtatcagc agaaggtcac cgacttgaat cgtgaagtgc
    74161 aggataagca gaaagaggtg ttggacggat tccgtgaaaa aattgaaacc ttgtcgggcc
    74221 gagtggccaa acggttggaa ctccaggccg tgttcgatcg cgggcgcggc gggcccactc
    74281 tgtacttcga cgaggcggtg gatgtgtcgt cgcaggtgat cgaagaattc aataaaacgt
    74341 atccatgatg agggggccca tgaacgtacg tacttggatc gtagcgggtg cggtgccggt
    74401 ggtgttggcg tggttggcgc agcccgtgca ggcagcggac cgcatcaagg tcgcgatgat
    74461 ggaccagcaa caggtcgtcg agcggagcgt ggccgggaaa cgcgcccttg aagatctcaa
    74521 aagttattcc acgacgcgcc agaagatcat cgattccgac gaccaggaat tgaaggagct
    74581 tgagaaggcg ctccaggaca gcgctctcac cgaagaggtc cgcaaggaga aacaggagca
    74641 attcagggcg aagctggagg cctatcagcg ccggattcaa gacttcaacc gtgaggtgca
    74701 ggagaagcaa aagggcatgg tcctggattt tgcgcagcgc atccatgccg cggtatccgc
    74761 cgtggcccag aaggaagggt ataccgccgt catcgaccgg ggcagcgagt ccaccgtcaa
    74821 gatcgtcgtc tacggccacc cgtcggtcga cctgacggag cagatcgtca aggagttcga
    74881 ccggcaaaac aaatagccgg gatgtgtaat cgtctacgca acacaggagg cggggctgat
    74941 gtcagtgatg gacaatgttg agattcaatc gatccttccc caccggtatc cgtttttgct
    75001 ggtcgaccgt attcgcgaac tcgatccgga ccgccggatc gtcggcatta agaatgtgac
    75061 gatcaacgag ccgttttttc aggggcattt ccccggccgt ccggtcatgc ccggggtgtt
    75121 gattcttgag gcgctggccc aggtgggcgg ggtgctggcg ttcaagtcgt tggggtcggt
    75181 cggccggccg gtcgtgtatt tgaccggcat cgacggggcc aaattcagga agccggtggt
    75241 cccgggtgac attctccgct tggaagtgga tgtgctgaag aagcgcgcgc cgttctggaa
    75301 gatgcaaggc agggccttcg tcgagtccga actggtgtgt gaagcggaag tcacggccat
    75361 ggtcacggat gaacaggccg gcggggacaa gtagccgggg ccctgtgtcg gccggtggcg
    75421 tggcgttgaa cagcatagca gtcgatggtg aggaggtttt gagtgaacat acatccgacc
    75481 gcggtggtcc atcccaaagc ggtgctcgca gacgatgtgg aagtgggcgc ctattccgtc
    75541 gtcggtgaac acgtccggat cggcgcaggc actcgcgtat tgtcccacgt gtgcatcgat
    75601 gggtggaccg acatcggaga gcggtgcgaa ttgcatccct ttgtatcggt cggcggaccg
    75661 ccccaacata tgcagtataa gggcgaaccg accaaggtcg tgatcggtca cgacaatatc
    75721 ctgcgggagt acgtgacggt caaccgggcg acggtgcaag gcggtggagt caccagtatc
    75781 ggcgacgcca acttcctgat ggcctacgtc catgtcgcgc acgattgcca cctcggcaat
    75841 catctcattc ttgcgaatgc agcaagtctg gccggccata ttacgatcgg cgatcacgcc
    75901 atcatcggcg gactttcggg catccaccag ttcgtgcgga tcggcgccta tgcgatggtc
    75961 ggcggttgct gcgcactcgg ccaggacctg ccgccgttca tgcgggctgc cggcgggtat
    76021 cgtgcccgta tgtacgggct gaactccatc ggtctgcgac ggcatggttt ctcctccgag
    76081 cgcatcgcgg cgctgaagaa gtcctacgaa gttctgtttc gttccgggca ccgggtggcg
    76141 gaggctgtca aactggcgcg tgagaacttc agtgccagcc cggatgtcat gcaggtggcc
    76201 gcgtttatgg aagggaccaa acgggggatt tgccggtcgg tcggtaagga tcaagagggc
    76261 gaagaggagt agcgggtggg gaagcaggtg gccgagcata ggcaggcatc cgatggacag
    76321 cggatcggac tgattgcggg taacggccgg ttcccgatca tttttgccga caatgcgaag
    76381 cggctcggat attccgtgtc cgcggtggcg catgagggcg aaaccgaccc ggaactcgcg
    76441 cggcacgttg atcacattca ctggatcaag atcggccagt tcggcaaatt gatcgaggcg
    76501 ctcaagggcg acggcgtgca gcgcgcagtc atgctgggcg gtatcaagaa gacgcacgta
    76561 ttttccacgg tccggccgga ctttcgcgcc ttggccctgg ccgctaaact gattcatctc
    76621 aaggacgacg acatcctgcg ccgggtcgcc gaggagatcg aacaggaagg tattcagatc
    76681 tgtgaatcca cctttgggct ggaagggatc cttgtcgagg aaggcacgtt gacaaggcgc
    76741 gagccgagca aaaaggaatg ggaagacatc cggtacggct gggatgtcgg aaaacagatc
    76801 ggagcgttgg atatcggcca gtgcgtcgtg gtgaaggacc gggtgattgt ggccgtcgaa
    76861 gccgtggaag gaaccgatgg cgcgatccgc cggggcggtg acctggcgca tggcggcgcc
    76921 gtggtggtga aacggtgcaa accccagcag gatctccggt tcgatcttcc tgccgtcggc
    76981 ccgcgcacca tcgaggtgat ggagtccgtg aaggcgtcgg tgctggcgtt ggaagcggga
    77041 cgcgggattc tcctggaccg tgacgaaacg atcgcgaagg cgaaccgtgc cggcatcgcg
    77101 attatcggaa tgacgtaaca ggctgcggaa aaactcgatt cgtgcaccta cgcagcgatc
    77161 ggcgtctgta aggtgtcggc attgaatata atacacaggt tgcgaaaaaa ggccgtccgg
    77221 caaggccgca gcaagcgacg aggtgaatcg tactcgggct gtacggtgag ccccggcgcg
    77281 atgcgcgaac gccgctggcg gactttttcc gcatcctgct catgtcgttc ggcattgaaa
    77341 cggaggcctg cctcgtgaga caggactggg cgcgatgaaa cagcttcgag ccggcgtgat
    77401 cggtgtcggt cacctggggc agcaccatgc ccgccactat gcgactctcc ctggcgcaac
    77461 gttggtcggt gtgtgcgatg cctcgccgac ccgcgccaag ttgatcgccg atcggcatgg
    77521 cgtccagttc tggacggacc tcgacgacct cctcaagcag gtcgatatcg tcagtgtcgc
    77581 cgctcctacc tctgcgcatt tttcggcgac caaggcctgc ctggaagccg gcaagcatgt
    77641 gctggtcgaa aaaccgatcg ccgtgacgtc ggcggaagcc cgtgaactgg tcgaactggc
    77701 tgcgcgccgt gggtgcctgt tacaggtcgg acatagcgag cggttcaatc cgatcatgca
    77761 gcgcatgcgt cccttcatcg aacgcccggc ctttatcgaa tgtcaccgtt tgagcgcctt
    77821 cggtgagcgg gggaccgacg tcgatgttgt gctggatctg atgattcacg atctggatct
    77881 ggtgttgtcg ttcaatcctg ggccggtcga agaggtgcgg gcggctggcg tgccggtgct
    77941 ctcgtcgaac atcgatatcg ccaatgcccg aattgccttt gcgagcggtt gcgtcgcgaa
    78001 tgtgacggcg agtcgcgtat cgaccaacaa gatgcgccgg ctgcgtgtct tccagcgcga
    78061 ccgttacgtc tcgatcgatt ttcaaacgcg ccaggctgtc gtgtcccgtc gcgtgcaggc
    78121 ggccggcgcc aagccgacca tcgacatcga atcttaccag gccggagatg aggagccgtt
    78181 gcggctcgag ctggattcgt tcattcacgc ggtgaatacc gggacccgtc ccgtcgtgtc
    78241 cggtgaagac ggggaagcgg cgctcaatct ggcgaccctg gtcctggagg ccatcggccg
    78301 gtttacgcaa cgccattcgg cggatgaggc tgcggcggtg gcctttggcc gggagaatgt
    78361 gtaagggtat gacctctgtg ccgatctgca cgagacgagc agcatgccac gcatcttaat
    78421 cgtcaccggc gaggcttccg gcgacctcca tggagctcac ttggtcaagg cgctcaagga
    78481 gctgtccccc gcccttcaga ttgtgggtgt cggcggcgcg tcgatgcggg cggccggcgc
    78541 ggaactcgtg aaagatattc cccagctgga tgtgatgggg ctcatcggcc tgtctgccgt
    78601 caaaacgatg ctgcagcgga tttcgcgtat ccggacactg atcaagggtg aacgctggga
    78661 tctcgtcgtg ttgatcgaca acccgggcct caattttcat tttgcgcgag tagccagggc
    78721 ctgcggtctc aaggtgctct attacattgc gccgcaagta tgggcctggc ggcgggggcg
    78781 gatgcgctgg atccagcagc gcgtcgatca tgtgctcgcg attctgccgt tcgaggaacc
    78841 gctctacaaa aaggccggcg tgcggtgtac gtttgtcggc aatccgctgc tcgacgaagt
    78901 cgcgccgtcg tacgatcggc aggccttgcg ccggcagttc ggactgtcgg atgcaggccc
    78961 cgtgatcggc ttgtttccgg ggagtcgaaa aggtgaactg ctcgagcata ttccgctcct
    79021 cctggagacg gtgaagcgtc ttgcgcaacg gcacccggcg attcagttca tcctggccca
    79081 ggcgtcatcg attcaagacc agttccttgc cgatctcttg aaagatagcc cggttcccat
    79141 tcgggtcttt cgcaatcagg ccagtgaagt catggcggcc tcggacctgc tggtcgtcaa
    79201 gtcgggcacg tccaccttgc aggctgcggt ggtgggaacg cccatgattt tgttctatcg
    79261 ggcctcatcc tggctcacct accggttggc gcgtctcctg attcgggtgc cctggatcgg
    79321 tctcgcgaat ctggtcgccg gacggggcat tgtgccggaa ctgattcacg atgaggcgac
    79381 gccggagcgg ctcgtacagg aaaccgaacg gctgttgggg gatcctcgcg cgtatgaaga
    79441 aatgaaggct gcgctcttga cggtacgcca ggcgttgggc acacccggtg cgtcgcgtcg
    79501 tgccgcggaa gccgtcctgg ccgagtgtcg ggcatgaaac attatcttcg tctcctcaag
    79561 tatctgaatc cctaccggtt tcgactgggc gcggcgtttc tttgcgcgct gctcgtggcc
    79621 ggactctccg ccgcctatgc ctggttggtg cgcccggtcc tcgacggcct gttcatcagc
    79681 aaggatgaaa gcctgctgct cgtgcttccc gtcgcaatcc tggcggtggc ggtgctcaag
    79741 ggggtgttca actacggaca gaactatctg atgaactatg tcgggaacca ggtcatcggc
    79801 gacatcagag agcagctctt ttcaaaactt gttcgactgc cggttcattt tcacgacacg
    79861 aatacgtcgg gccggttggt ctcgcgggtc attaacgatg taaaccagat ggccaatgcc
    79921 gtggcggggg tgctgaaaga tctgtttcag cagggactga cgtttctggc catgatcggc
    79981 gtgatcattt accagaactg gaaactggcg gcggtctcga tgatcgtggt gccgctttcc
    80041 gtggtgacca tggcgagaat gggaaaacgg ttgaggaatc tggcgacgcg cggccaggaa
    80101 cgcatgggcg acatggcctc gacgctgcag gaaacgctgg ccggtatccg catggtgaaa
    80161 tcgttcggcc gggaagagga agaagccaag cgatttcgtt tgagcaacga cgccttcatc
    80221 catacgacga tgaaggccat tcaggtctcc tcgctgggtt cctcccatat ggaggtcatc
    80281 ggtgtgctcg gggtcgcggg gatcatctgg tacggcgggt atctggtcat ccatgatgag
    80341 atgactcccg gcgccttctt ctcgttcctg gcggccatgt tcatggccta cacgccgatc
    80401 agaagattgt ccggcgcgaa caacacggtt cagcaggcgc tggcggccgc cgagcgggta
    80461 ttcgatgtgc tggacttgcc gacggagccg gcggccaacg gcggccagac ggacctgccg
    80521 gccatttcca ggtccctcga attccggcag gttgcgttcc agtacgaagg ccagggtgag
    80581 cctgcgctga gcggtatcga tctgacgatc cgggcgggcg agatcattgc ctttgtcggc
    80641 agcagcggga gtggaaagac cacgctggtg agtctgctcc cgagattcta cgatccgacc
    80701 ggagggcaga ttctgatcga cggggtggat cttcagacct gcagtttgcg gtcggtacgc
    80761 gggcagatcg gaatcgtctc ccaggaagtc gtgctgttcg acgatacggt gcgcaacaat
    80821 atcggctacg ggcggcaggg tgccacgccc gacgatgtga tgcgagcggc ccagctggcc
    80881 tacgcgcatg agttcattgt ccggatgccg gccgggtatg atacgttgat cggagagcgc
    80941 gggctcaagc tctcgggcgg tgagcggcag cggttggcga tcgcgcgggc gattctccgc
    81001 gacccgccga tcctgattct ggatgaagcg acgtcggcgc tggacaccca atcggagcgc
    81061 atcgtgcagc tggccctgac caatttgatg cataaccgaa cgaccctggt cgtggctcac
    81121 cggctgtcga ccattcagaa tgccaaccgc atcgtggtgt tggaccgggg cagggtcgcg
    81181 gaagtcggct cgcacgaaga gctcctgcgc aagggcggca tgtataagcg cctgcacgcg
    81241 atgcagtttg ccgatgcgat ctcggagtag ggacgcaggc gaaggtgagc gatggcagtc
    81301 ctatgacaac tgatcgacca gcggcggccc ctcagacgca gcccttgctg aagcgagtcg
    81361 tcaacgcgct caaggtcgcg gtggtgccgc ccgtcggcta ccagatcatt cagatgctac
    81421 gccgcacgat gacctggcgc accgaaggct cagagcatgt gaaccgcttg tttgcagaag
    81481 agaagcgggt gatcctggcc ttctggcatg ctcagcagtt gatgatgccg ctggccattc
    81541 cggggcttga agcccatgtg ttgatcagtc agcatcgcga cggcgaattg atccgccgca
    81601 tcgtggcacg gttcgggttg gacgccgttc gcgggtccag taccagaggg ggtgcggaag
    81661 cctttcggca gctgattcgc ctcgggcggt ccggcggcaa tctcgtgttg acgccggacg
    81721 gaccgaaggg cccgcggcag gtggccaagg tgggcgtagt gcagctggcg agggccaccg
    81781 gcctgccgat catcccgatg gccttcggct gctcaaaaaa aaactcttcg cgagctggga
    81841 tcggttcatc gtgccctatc cgttctcgcg gggcctcttt ctcctggggc cgccgatcag
    81901 cgtgccgccc gacgcctcgt ccgacgacct tgagcatgtc cgccgggagt tggaagacac
    81961 gctgaaccgc atgacgatca aggccgatga ggccgtcctg cgggctggcc cgtgatgtgg
    82021 tatctgctct acaacagtct tctactcctg gtttccccga ttatcctgtt cgtcttgttg
    82081 gccaagcagc gttgtcgccg ggggttgccc caacggctgg gactgcgggc ggagatgccc
    82141 acgattgatc cagccggcag gtcgggttgc atctggattc atgccgtgtc tttgggcgag
    82201 gtggtcgccg tggcgccgct tgtgcgggat ttgcgtcggc ggtaccctga gacgaggttg
    82261 gtggtctcga ccgtaaccga aaccgggcgt gaagcggtgg agcaacggct ggagggtgtg
    82321 gccgagcacc ggtacgcgcc gctcgatttt ccctgggtcg tcaatcaggc gatcgaccgt
    82381 ctcaagccga gtctgtacat ttttgtcgaa acggaactct ggccgaatat cctgcggagt
    82441 ttgcggcggc gcaatgtgcc gtcgattctg gtcaacggac gattgtccac ccgttccttt
    82501 gagcggcaac gcctgccggt catccgggat ttctaccgga ccatgctgaa catgatcagc
    82561 tgttgcctca tgcaatcgga gcgggatgcg cagcgcatga tcgacttagg ggccgaaccg
    82621 tcgcgggttc gatgtaccgg caacatcaag ttcgatcaac cggttcctca ggctgccgcc
    82681 ggcggcacgg ccgtgtcgaa agcggcgctc gggctgaccg accgggaact gttgctggta
    82741 gccggaagta cacatcccgg cgaggaagaa acgatcgtca atgcctatcg gatcctgagt
    82801 cctgagttcc ctgagctccg cttggccctg gccccccgcc atatcgaacg ggcggcgcag
    82861 gtggagcaga tgatccaggc gaaaggcctc acggtgaccc ggcgtagcac cggcggacaa
    82921 gtgccgatgg cgagcacggg ggcgcgggtg ttggtcctgg atacgcgcgg ggaattggcc
    82981 ttgttatatc gagacgcagt ggtggccttt gtcgggggca ccctggcgcc ggtgggaggg
    83041 cataatctct tggagccggc ggtctggggg aaaccggtgc tgttcggtcc gcataccgac
    83101 cattgtgccg aagtggcggc gctcctgctg aatgcgcagg gaggactggt ggtacaggac
    83161 gaacaggacc ttgcgcaggg cctgcgagcg ctccttcgcg atcccgccgc gttgctgcgg
    83221 atggggcagg cggcgcagca ggtcgtggcg gacaaccagg gtgccctcca gcgcagcgcg
    83281 gagatcatcg ccacgttttt gccggcacga gggacctcga agaacgcgcg ggttgaacag
    83341 gggcgggagt tgtcgagtcg gcaatcgtaa tggcggcgac catgtcgaga gggctgcggt
    83401 cctggttgcg gtgggtcggg tatccctacg aactcgcggc tcgcctccgg ctgtggggtt
    83461 acacccatgg ctggttctcc acgcgtcggt tacctcgtcc cgtcatcagc gtcgggaacc
    83521 tcaccgtcgg tggcacaggc aaaacgccgg tcgtcatgta cctggtggag cgactgacgg
    83581 cacagggcaa acgcgtggcg attctgagcc gcgggtatcg gcgtcgcagc aacgctccgc
    83641 aattgctggt ctccgacgga caacaggtgt tggtggggcc ggacgaggcc ggagatgagc
    83701 cctatttgat cgcgcgccgc tgccctcagg ccgtcgtggc ggtgggggcg aaccggtatg
    83761 cgctcggcca atgggtcttg gggcgactgc cggtggattg ttttgtgctg gacgacgggt
    83821 ttcagcatgt ccaactccat cgcgatctga atctactcct ggtggacgcg acggacgcgg
    83881 cgggcatcca ggcggcgttg ccggtcggac gtttacggga acccctctcg gcggccgcgc
    83941 gcgcctcggc catccttatc acccgggtcg atgaggcgca tggaggggag tcggtgcggt
    84001 gtctgttgct cgacgcctgc ggctcacttc cgtcgctggt gcgtgtggga tttcgggcgg
    84061 aggagttccg gcgcgtaggg acgggcgaac ggcttccgtt ggatgcgttt cgcggccggt
    84121 cggctgtgct cttcagcggg atcggcaatg cggaatcctt tcgcgccctg gtggccggat
    84181 taggcatcgc tgtgattgag atgctggcat ttcccgatca tgtgcactat acccgtggca
    84241 tgatagacac cattcgagcc aaggcgaaag cctctggcgc cgatctgctg gtgacgacgg
    84301 agaaagatgc cgacaaggtt gcgccgttgt tagttccgga ggatgcctgc tgggccgtga
    84361 gactccgcac cgaaattgtg tcggggcagg agaatttgga gcgactgttg cgactcgact
    84421 cagcaccagg caggacggga ggccatgcgt aaggaaggca tccgtcgcat cgtcgtccgc
    84481 ggccccaatt ggctcgggga tgcagtcatg tgcgaaccgg ccttgagtca ggtgcgcacc
    84541 ctgttcccgc aggccgagat taccctgctt gtgaagcccg gcattgcgga tctgctggcg
    84601 caacatccgg aggtgaaccg gacgctggtg tatgacgatc ggggacgcca tgccgggctg
    84661 gttggaaaat ggaccctcgc cgcggtgctg cgccgtcacc gattcgatct ggccatcctg
    84721 tttcagaatg cctttgaggc ggcgctgatc agtttcctgg cgggcattcc cagacgattc
    84781 ggatatgcga cggacgggcg aacgctgttg ctgaccgacc cggtgaccgt gccgccacgc
    84841 actgcgcaac gacatcaggt cgagtattat tgggatctcc tgaagccgct gggcgggcac
    84901 ggtcctgcgc ctgcgcctcg tttgtttgtc acgccggatg aatcggcgtt gatcgccggg
    84961 cggttggcgg atgccggaat cgggccttcg gatccggtga tcggggtgaa ccccggctcg
    85021 acctatggcc acgcgaagcg atggttgccg gatcggtacg ccgaggtcgt caatcgtgcg
    85081 gtgacggatg tgcaggggcg atcgggtgcc agagtcggcg tggcgatctt gggagccaag
    85141 ggtgaggagc cgttgggaaa agccatcgcc ggccagatca agacgcgcac ggtcgtgtgt
    85201 tccggccaga ccacggtacg ggaattgatg gcgctggtga agcgctgcca gttgtttctc
    85261 acgaacgaca cggggcccat gcacgtcgcg gccgccttca aggttccgct ggtagccgta
    85321 ttcggcccga ccgattggca aaccacgtcg ccgttcggcg tcgatgccca gctggtccgg
    85381 cagccggtgt cctgcgcgcc ctgtctcttg agggaatgtc ccatcgatca ccggtgcatg
    85441 accggggtga cggtggagca ggtctacgat gcagtggtgc agcacctgcc gctggtggcc
    85501 cctcctccgg cagtcgaccc cgcgccaccg acgccaggtt tcacttccac gtcgctggcg
    85561 ggtgtgaccg tatttctcga tcgcgacggc acattgaacg tcgatacggg ttatgtgaag
    85621 tctcccgatg acttcacggt tctacccggc gtgggggcgg cgctggcccg gttgaaacag
    85681 gccggggcgc gcctggtcgt cgtcacgaat cagtccgggc tggggcgggg gtattttacc
    85741 tcaagggatc tcgaagcgat tcacagcaag ttgcggctgg tgctggctga ggacggggtg
    85801 acgctcgatg gactgtattt ttgtccccac catcccgacg atcactgcaa ttgtcgcaaa
    85861 ccggcccgag gcatgatcga ccgcgctcat gccgaattgc aggtggacct gagccgatcc
    85921 tatgtgatcg gcgacagtgt tcgtgatgtc gaattggcca agcaggtggg ggcgcagagc
    85981 ctgctggtga tgaccggccc gtccggtgcg gaggccctgg ccgacttgac ggtgcgcgat
    86041 ctgcctcctg actacgtggc cgaggggttg tcgcaggcgg tggactggat cgtcgcccat
    86101 gcgacgcaca ggcccgccgc cgacatgcag cgctgatcat ctctggtggc cgaatgaccg
    86161 actccgtacg ccgactcctg ctgatcaaac cgagttcgct gggcgacatc gtgcatgcga
    86221 tgccgaccct ggcggcgttg cgcgagcagt ttccccaagc ccgggtcacc tggctggtga
    86281 agcgacaatg ggcgccgctg gtggaagtca ttgccggggt cgatcaggtc tgttcggtat
    86341 cggaaggact caggggctgg ttgggtcggg tgcccgatct ccgggcggcc ggcttcgatc
    86401 tggtcgtcga tcttcaagga ctctttcgga gcggggcgat ggctcggttg agcggttgcg
    86461 accgtcggat cgggtttgcc aatgcgcggg agggcagctc tctgttctat acccagcggg
    86521 tggcggttcc tcccgcgccc atgcacgcgg tcgatcgcta cctgctcgtc gccgaggcgc
    86581 taggcgcgac acgtccaacg cagccgcggt tcgaattcgt cgatcgagcc gaggaccggc
    86641 aggccgtgga gaccatgttg tcccgggccg gagttgcccc tcatcaacca tgggttgcca
    86701 tgaacgtgtc cgcacgttgg gagaccaagc gttggccacc gcaacatttt gctgaggcag
    86761 cagaccggct ttctcaggcg catgcgctgc ctgtggtcct gatcggcggg ccggccgagc
    86821 gggcggagtc gctcgcggtg acggcgctca tgcgtacgaa agcgattgat ctgacaggac
    86881 agacccctgt aggcttgctc cccggtttac tccgtcgcgc gtccttgttg gtgacgaacg
    86941 attccgggcc catgcacatc gctgcggcgg tggggacgcc ggtggtggcc ctattcggcc
    87001 caacggatcc ggtcaaaacc gggccctatg gcaagggcca tgtcgtcctg tcgcatgcgg
    87061 tggaatgcag accctgtttt cgccgtgact gtgcccctgc cgtgcccctc gaatgtctga
    87121 ccgccgtgcg accggaacag gtcgttcgtg ccgtcgaaca acaacttgag cgatcacaga
    87181 aaccaggatc attgtgaaca tcatcattgc ggaatccagc acggcggtcg gcgggcagga
    87241 actggccgtg ttgctgcacg ctgaacgtct gttgaaacgc ggccatcgta ttcgattggt
    87301 cctggagccg cgcagcccca tcatggccat ggcgagggaa cgagggctcc cggtcgaacc
    87361 gtttgtcatg cggcaatggc ggctgccgct gtcgattctg gctttccgcg cgttgctcaa
    87421 gcgggagcgt cccgagatcg tccacgtcaa cagttcacgc gatagctgga tcgcggcgct
    87481 gtcgtcgcgc ctgctcgatc cgcgccccaa agtcattcgc acgcggcata tttcggcgcc
    87541 gctcaataac aacgccacca cgcattggct gtatcgtcgt ctcttcgaca tggtcatcgt
    87601 gaccgggagc gaacggaatc ggcaggacct catccatcgg gacggattgg cgccggaccg
    87661 tgtcgcttcg tttcccatcg gcttggatgt ggagcacttt tcgccggcca agcctcagca
    87721 cgatatccgg tccgagctgg ggatttccac cgggcatctg ctggtcggga tgatttccta
    87781 tctgcgggac tataaaggcc accggtatct tgtagaggcg gccgccaagg tgctgaagca
    87841 acaccaggga gtggcctttc tcatcgtggg tgaagggccg gaagagcaga acatccgcgc
    87901 gcagatcgaa cgcctcggct tgaccgccgg cgtgcgcatg ttgggattcc gagatgacct
    87961 cctggatgtg ttccggtcgt tgaatctctt tgtgattccc acggtcgaag gcgataccat
    88021 tcctcaggtg ttgatgcagg ccctggcgat cggactcccg gttgtctcga cgaccaccgg
    88081 atcgattccc gatgtgctgg ccgacgggga gtcggggttc atcgtcccgc cgcgggatgc
    88141 cgatgcgttg gccgatcgga tcggtcgcct gctggtcgat cccgagcttc gcgccgcgat
    88201 ggggcgacgg ggccgtcaga cggtggagca gtcctattcg atcgaccgga tggtggatga
    88261 gctggagcgt gtctatcgac gggtgatcgc atcatgatgc aacgatgggc ggaagcccgc
    88321 gcctccatcc gcaaaagcgt gctgttctgg tggattctct tcgtcgtgat gcaaatggcg
    88381 gagcgggtgt ttctgttgcg cgacgcgttt gagcaggaag ctcccagcct gccgctgctg
    88441 ctcaagaccc ttgtcgtcgg cgtgcggggg gatttcatta ccgccacctt tgcgttggta
    88501 ctggccgggg tcggggcagg ggcctgggcc ctgcggcccg cccccggccg tcgcaccggc
    88561 aggggggcgg tattgccgga ccccccaccc ccgcgccaca ggtgcggagg cgcacccggc
    88621 cgcgg
//
LOCUS       DAORRQ010000009        86025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_9_length_86025_cov_267.742654, whole genome shotgun sequence.
ACCESSION   DAORRQ010000009 DAORRQ010000000
VERSION     DAORRQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 86025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 86025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9_length_86025_cov_267.742654"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /gene="rplE"
                     /locus_tag="PK224_04515"
     CDS             complement(<1..370)
                     /gene="rplE"
                     /locus_tag="PK224_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRB15033.1"
                     /translation="MKEFSYGNLMQVPRLERIVLNVGMGEAIQNVKLLESASNELGII
                     TGQKPVTTRAKKAIAGFKLRQGMPIGAKVTLRSRRMWEFLDRLISLALPRIRDFRGIS
                     PKAFDGRGNYTLGLKEQLIFP"
     gene            complement(553..879)
                     /gene="rplX"
                     /locus_tag="PK224_04520"
     CDS             complement(553..879)
                     /gene="rplX"
                     /locus_tag="PK224_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRB15034.1"
                     /translation="MARIKTKIRKGDTVVVVTGRERGKSGKVLSVDTINGKVLVEKLN
                     MIKRHTKPNQKLRQGGILEREAPLAISNVMFLCPVTKKPTRLGVKRQEDGRRARLSKK
                     SKEIVE"
     gene            complement(906..1274)
                     /gene="rplN"
                     /locus_tag="PK224_04525"
     CDS             complement(906..1274)
                     /gene="rplN"
                     /locus_tag="PK224_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRB15035.1"
                     /translation="MIQNYTYMDVADNSGAKQVMCFHVLGGTRRRYGSLGDIVVVAVK
                     EAIPQAGVKKGDVSRAVIVRTTKEVRRDDGSYIKFDRNACVLINAQGEPVGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            complement(1335..1604)
                     /gene="rpsQ"
                     /locus_tag="PK224_04530"
     CDS             complement(1335..1604)
                     /gene="rpsQ"
                     /locus_tag="PK224_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRB15036.1"
                     /translation="MKEGQQHRREWYGNVVSNKMNKTVVVAVERSVIHPIYKKVLRRV
                     TKLKAHDEGNVCKVGDRVQLSETRPISKDKHWRVVRVMVKGQPEK"
     gene            complement(1619..1840)
                     /gene="rpmC"
                     /locus_tag="PK224_04535"
     CDS             complement(1619..1840)
                     /gene="rpmC"
                     /locus_tag="PK224_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRB15037.1"
                     /translation="MDVKDLSGLSIDELAEKEKQLRHELFNFRFQLGIGRLENPMQVR
                     STKRDIARLKTVRRQLELLQTQADQPVAK"
     gene            complement(1869..2285)
                     /gene="rplP"
                     /locus_tag="PK224_04540"
     CDS             complement(1869..2285)
                     /gene="rplP"
                     /locus_tag="PK224_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRB15038.1"
                     /translation="MLAPKKVKFRKMQKGRMRGKAYRGGAITLGEFGLKALEPGWVTS
                     RQIEAARIAITRFVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     MYEMGGVAPDVAKQALKLAAYKLPIATKFVVRGEFQ"
     gene            complement(2314..2982)
                     /gene="rpsC"
                     /locus_tag="PK224_04545"
     CDS             complement(2314..2982)
                     /gene="rpsC"
                     /locus_tag="PK224_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRB15039.1"
                     /translation="MGQKTHPIGYRIGYNYTWSSRWYADKDYARLLHQDIKIRKMVKA
                     KLYHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKAALEKEYSGQAYITV
                     KEIKKPELDAQLVSENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMVAGRLGGAE
                     IARTEWYREGRVPLHTLRAEVDYGFAEAHTTMGQIGVKTWIYKGELLPALQLKPDSAL
                     GRLG"
     gene            complement(3015..3368)
                     /gene="rplV"
                     /locus_tag="PK224_04550"
     CDS             complement(3015..3368)
                     /gene="rplV"
                     /locus_tag="PK224_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRB15040.1"
                     /translation="MAEAKAILRFVRVTPRKARIVIDMIRGQQVPMALAMLRHTPKHA
                     ARVIEKLLRSAVANAEQKELGDSDEMWVSQAVVNCGPIYKRFRARSMGRANSIQKRTS
                     HITIAVAAPGAAVNS"
     gene            complement(3413..3700)
                     /gene="rpsS"
                     /locus_tag="PK224_04555"
     CDS             complement(3413..3700)
                     /gene="rpsS"
                     /locus_tag="PK224_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRB15041.1"
                     /translation="MPRSITKGPFVDDHLMKKVEQMNQTKDRKLIKTWSRRSTVVPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGQAKTEKAVALK"
     gene            complement(3712..4539)
                     /gene="rplB"
                     /locus_tag="PK224_04560"
     CDS             complement(3712..4539)
                     /gene="rplB"
                     /locus_tag="PK224_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRB15042.1"
                     /translation="MALKVYKPTTPGRRGMTAIQGEKLTKKKPEKSLTGFHLRTGGRN
                     NDGRQTIRFRGGGHKRLYRQIDFRRDKAGIPAKVASIEYDPNRSSRIALLHYADGEKR
                     YILAPVGLSVNDEVQSGVGAEVRPGNALPLVNIPLGTTIHNIELKPGKGGQLIRSAGG
                     SAQVMGRDGAYVQIRLKSGEMRKVLSTCMATVGQVGNVDHENVVVGKAGRTRWKGKRP
                     HVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGTPAKGYKTRKNKATDKFIISRRKK"
     gene            complement(4573..4866)
                     /locus_tag="PK224_04565"
     CDS             complement(4573..4866)
                     /locus_tag="PK224_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRB15043.1"
                     /translation="MKGDLHQVLIQPLLTEKITALREQGNTVGFLVHPDANKIQIRQA
                     VESLLKVKVSRVNVVNIRGKVKRLGRFVGKRSDWKKAFVTLKEGEKLELFESV"
     gene            complement(4863..5486)
                     /gene="rplD"
                     /locus_tag="PK224_04570"
     CDS             complement(4863..5486)
                     /gene="rplD"
                     /locus_tag="PK224_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRB15044.1"
                     /translation="MPIVDVVDSKKKKVGTVDLPSEVFGCKPHVPLVHEAVVMQRACD
                     RQGTASTLRRGEVSGSGKKPWKQKHTGRARAGSLRSPVWRHGGTVFGPKPRSYAFGMP
                     RKKYRAALQSALSAKVLEGNVLVVSDLAINQAKTKLLANALSQLGISGTALLVVGDAN
                     SHLVQAGKNLSNVTVLRPEELNVYDVLRCHSLVIPQSEVNRVKEVWS"
     gene            complement(5490..6110)
                     /gene="rplC"
                     /locus_tag="PK224_04575"
     CDS             complement(5490..6110)
                     /gene="rplC"
                     /locus_tag="PK224_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRB15045.1"
                     /translation="MTNGLLGKKLGMTQVYDESVLAPVTVIEAGPCRVVSVKTKERDG
                     YEAVQLSFGEVKEQRLSQGELGHLKKHQAPPSRWLREFPKVGDVTVGQTIKVDMFKKG
                     DWVDVVGVSKGKGFQGVVRRHNYAGGPETHGSMFHRAPGSIGASSYPSRVWKNKALPG
                     HMGDERVTVQRLKVVDARPDENLLFVRGAVPGGVNGLLIVRKSKKS"
     gene            complement(6153..6464)
                     /gene="rpsJ"
                     /locus_tag="PK224_04580"
     CDS             complement(6153..6464)
                     /gene="rpsJ"
                     /locus_tag="PK224_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRB15046.1"
                     /translation="MKVDQRIRIRLRGFDYRVLDQSVVEIVETVRRSGARVVGPIPLP
                     TRIEKFTVQRSTHVDKKSREQFEIRTHKRLLDIMEPTPETMDSLMKLNLAAGVDVEIK
                     L"
     gene            complement(6505..7710)
                     /gene="tuf"
                     /locus_tag="PK224_04585"
     CDS             complement(6505..7710)
                     /gene="tuf"
                     /locus_tag="PK224_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRB15047.1"
                     /translation="MAKAKFERRKPHVNIGTIGHVDHGKTTLTSALTKICADRGMAKF
                     ISYDEVAKASESQGRRDASKIMTIAISHVEYETDNRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADKVDDKELLELVELEVR
                     ELLTKYDFPGDKIPIVQGSALKAVEGDQGPLGVPSILKLLEAVDTYIPTPTRAIDKPF
                     LMPIEDVFTISGRGTVVTGRCEKGIVKVGDEIEIVGLRPTQTTIVTGVEMFRKVLDEG
                     QAGDNIGVLLRGTKKEDVERGMVLAKPKSITPHTKFKAEIYVLTKEEGGRHTPFFNGY
                     RPQFYFRTTDVTGIVTLTPGVEMVMPGDNVTVTGELISPIAMDQGLRFAVREGGKTVG
                     SGVVTEILA"
     gene            complement(7746..9827)
                     /gene="fusA"
                     /locus_tag="PK224_04590"
     CDS             complement(7746..9827)
                     /gene="fusA"
                     /locus_tag="PK224_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRB15048.1"
                     /translation="MARQTPLERTRNIGIMAHIDAGKTTTTERILYYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWREHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAAFDSVQGVEPQSETVWRQADKYEVPRIAFMNKMDRIGADFYASVQSIIDRLGANPV
                     PIQIPIGREAEFRGSVDLISMKGYFYDDETLGAKYKIGEIPADMVDQANEYRQKMLDA
                     VAEFDDQVMEKYINGQPLSEEEVRRVVRAGTIAMKVVPVLCGSAFKNKGVQQLLDGVV
                     DFLPSPVDIPPVIGVDPNTSKEVERRPSDSEPFSALAFKIMTDPFAGQLTFFRVYSGT
                     LKTGTPVLNVTKGTKDRVGRLLKMHANKREEIDIVYAGDIAAAVGLKNATTGDTLADE
                     KQPVLLEIMKFPEPVIAMAIEPKTKPDQEKMGFALQKLAQEDPSFRVRTDEETAQTII
                     AGMGELHLEIIVDRLMREFKVEANVGKPEVAFRETIRRKAEAESKYIKQTGGRGQYGH
                     VVMTVEPSESGKGLEFINKTVGGSIPKEYIPAIEKGVKERMETGVVAGFPLRDVKVTV
                     IDGSYHDVDSNEMAFKIAASMGFADACKKADPVLLEPIMKVEVLVPQDFMGDVIGNLN
                     GRRGKVQGMKVRAGAQAIEATVPLMEMFGYATDLRSRTQGRATYSMEFDRYDQVPRQI
                     AEAITAKHRGE"
     gene            complement(9941..10414)
                     /gene="rpsG"
                     /locus_tag="PK224_04595"
     CDS             complement(9941..10414)
                     /gene="rpsG"
                     /locus_tag="PK224_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRB15049.1"
                     /translation="MPRGQFFGHREAQPDSKYRDKLVGKFLNVLMGGGKKSTAERICY
                     GAFDLIQEKTNGGDPMKIFKSAIDNVKPVVEVKSRRVGGASYQVPVEIRPSRRVSLAL
                     RWITEYSRSRGGKSMQDRLAAELLDASNNTGASVKKREDVHRMAEANKAFAHYRW"
     gene            complement(10476..10847)
                     /gene="rpsL"
                     /locus_tag="PK224_04600"
     CDS             complement(10476..10847)
                     /gene="rpsL"
                     /locus_tag="PK224_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRB15050.1"
                     /translation="MPTINQLVRKGRTLVKSKTKSPALKRCPQKRGVCLRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGSL
                     DAVGVADRKQARSKYGAKRPK"
     gene            complement(10957..15090)
                     /gene="rpoC"
                     /locus_tag="PK224_04605"
     CDS             complement(10957..15090)
                     /gene="rpoC"
                     /locus_tag="PK224_04605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRB15051.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVMVDPGSTSMSEQELVSEEQLRSLQSEYGSGAFKV
                     GIGAEAIRELLRKVDINTQWDELHVKAKASASAALKKKYAKRLKVLEAFRRSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLIELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERVGAKGEGKL
                     FGSPEEARIAYDAGALDEHARIKVRVNGTMVQTTAGRVILAEILPPMMPFADANKLMT
                     KKEMSKLIDAVYRQAGHRETVTFLDKIKDLGFHYATRAGMSICIDNMHIPSRKEDLIG
                     KAQHEVNEIEKQYSEGLITNGERYNKVIDIWAHVTEQVANEMMKELGAGGDPAKIESF
                     NPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTH
                     GARKGLADTALKTANSGYLTRRLVDIAQDVIVTEEDCGTTDGILVSALLEGGEIIQPL
                     EERLLGRLAGEDIRDPVTGEIIVSFNEEIDEEQAKAVVEAGVDRVKIRSVLTCQSARG
                     VCRLCYGRDLSRGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLE
                     AKHAGHLKYMSLDAKKNADVHNAGIAVRNKEGEWVVMNRNAKIAIVDDSGREREKYPV
                     VYGAKIKVKDGDRVDVGQKLVEWDPYSLTILTETGGKVAYGDILEGVTMKEEFDEVTG
                     LSRKVIIEQSGATLRPRVSIKDDSGKTAKVSGSGGAPVARYLLPVGAHIFVEKGAMVH
                     PGDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEQAVISEIDGEVSYGGFVKGMRK
                     VLVDNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLMDGSANPHDILDVLGPKELQKYL
                     VDEVQDVYRLQGVSINDKHIEIIVRQMLRKVRIEDPGDTSFLPGSQVSKGLFDVENQR
                     VLEKDGKPALGKPVLLGITKAALTTDSFISAASFQETTRVLTEAAINGREDNLLGLKE
                     NVIVGRLIPAGSGFEEYRETFVASAKAPGELSGGQPQPVAVGQHAAGSAGADPENNG"
     gene            complement(15232..19191)
                     /gene="rpoB"
                     /locus_tag="PK224_04610"
     CDS             complement(15232..19191)
                     /gene="rpoB"
                     /locus_tag="PK224_04610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRB15052.1"
                     /translation="MSESTLSEFVERKDFSRIRTSIDIPDLIEIQKRSYEEFLQMEVE
                     PDRRKDQGLQAALASVFPITDYNNTAALEFSNYSLGTPKYDERECLEQGMTFAVPLKL
                     RVRLIVFDKEDKGPKKKVLDVREQEVYVGELPLMTERGTFLINGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGYSTDDLLRMYYPVEEIRVSKGKLYRKLDPEIHHGLKCSTEVMEKGGKDPLVREGAK
                     LTKVLIAKLKAAGIKEIPVTPAELVGRAVLTELVDSGKKQSLAEKNQRLTEEILEKIL
                     DSDIEEFKVIYLDTTNATLVILDTLDMERTGSKEEAMVEIYRRLRPGETPSVETARAL
                     FDNLFLSPKRYDLSPVGRLKLNKKLGLDFALEQRTLTAQDIVEVVRYLVNLKIGRGEI
                     DDIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVA
                     AVKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRI
                     CPIETPEGPNIGLITSLATYARINEFGFIEAPYRKVVKGRVSDELEYLSAIEGDKYII
                     AQANSKVDTSGRLVSETVSARSAGDFITATPDKIEYMDVSPKQVVSVATALVPFLEHD
                     DANRALMGSNMQRQAVPLLKAESPLVGTGMEAVVARDSGYVVQAKREGVVESVDATRI
                     VVRADAKEGRKRNDSGLDVYEMIKFQRSNQNTCITQTPVVRIGEPVKRGQVLADGPAI
                     DHGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGK
                     EDITRDIPNVGEEALRDLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAI
                     FGEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIESEDHMKLQRDHQEE
                     LRIIEDEKTKKVRKLLLGKVVGRDLMDPETGDVILKKKGKLTAEILKRLPDDMVRHII
                     LSDPDEQKELEDVERRAKEQIEILQTLYDEKVGRLRRGDELPPGVIKLVKVYIAMKRK
                     IQVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHL
                     GWAARALGIKVASPVFDGASEKEIKELLKKAKLSPSGQTMLMDGRTGESFSSPVTVGY
                     MYVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASIL
                     QEFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKSKD"
     gene            complement(19273..19674)
                     /gene="rplL"
                     /locus_tag="PK224_04615"
     CDS             complement(19273..19674)
                     /gene="rplL"
                     /locus_tag="PK224_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRB15053.1"
                     /translation="MSAAAGTKLSQDELIKAIEGMSVLELADLVKGLETRFGVTAAAP
                     VAVAAVGGGGAAAAPAEEKTSFDVILVSAPADKKIQVIKVVRELTSLGLKEAKDLVEG
                     APKPIKAGATKEEADTMKKKLEESGAKVEVK"
     gene            complement(19702..20208)
                     /gene="rplJ"
                     /locus_tag="PK224_04620"
                     /pseudo
     CDS             complement(19702..20208)
                     /gene="rplJ"
                     /locus_tag="PK224_04620"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            complement(20240..20932)
                     /gene="rplA"
                     /locus_tag="PK224_04625"
     CDS             complement(20240..20932)
                     /gene="rplA"
                     /locus_tag="PK224_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRB15054.1"
                     /translation="MGKKLTAAQEKIEPRFYGLKEAVEAVKQAAFAKYDESVDLAIRL
                     GIDPKRSDQMVRGTTALPHGTGKKLRVLVFAKGEKEQEARQAGADHVGSDDLMEKIKG
                     GWMEFDCAISTPDLMASVGKLGKVLGPRGLMPNPKTGTVTFEVGKAVSEIRKGRVEFK
                     VEKAGIVQVPVGKVSFDVQKLYDNAQAVLESVVKAKPSSCKGRYIKSVTMSSTMGPGV
                     KLDPVALSKLWS"
     gene            complement(20977..21399)
                     /gene="rplK"
                     /locus_tag="PK224_04630"
     CDS             complement(20977..21399)
                     /gene="rplK"
                     /locus_tag="PK224_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRB15055.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKDGDSIIPVIITVYKDRSFTFIMKTPPASDLLKKAAGIIKGSGVPHKDKVGKVSQ
                     AQVREIAQKKLSDLNAADLEGAIKIIQGTARSMGITVQ"
     gene            complement(21478..22020)
                     /gene="nusG"
                     /locus_tag="PK224_04635"
     CDS             complement(21478..22020)
                     /gene="nusG"
                     /locus_tag="PK224_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRB15056.1"
                     /translation="MMSNKNWYVIHTYAGFEGRVKTSLLERASQMGLTERLGQVLVPT
                     EDVIEIKDGKRRTSRRKFFPGYVLIELEAPLADETLQMIKETPKVTGFVGGGAQPTPL
                     SSEEVDSLLKQVDAGAAGPREQVRFIKGDNVRIVDGPFLGFNGAVDDVDADHSRVKVF
                     VSIFGRSTPVELGFLQVERI"
     gene            complement(22091..22285)
                     /gene="secE"
                     /locus_tag="PK224_04640"
     CDS             complement(22091..22285)
                     /gene="secE"
                     /locus_tag="PK224_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRB15057.1"
                     /translation="MFQRLIASIREFIDGVRGELKKVSFPTRAETVGATTVVIVFCIL
                     MSLYLSMMDSVLGWLMRKVI"
     gene            complement(22375..22449)
                     /locus_tag="PK224_04645"
     tRNA            complement(22375..22449)
                     /locus_tag="PK224_04645"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22414..22416),aa:Trp,seq:cca)
     gene            complement(22507..22656)
                     /gene="rpmG"
                     /locus_tag="PK224_04650"
     CDS             complement(22507..22656)
                     /gene="rpmG"
                     /locus_tag="PK224_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRB15058.1"
                     /translation="MRDIIDLACTVCKQRNYTTRKNKKNDPDRLERNKFCKFCRKHIA
                     HKEVK"
     gene            complement(22735..23940)
                     /gene="tuf"
                     /locus_tag="PK224_04655"
     CDS             complement(22735..23940)
                     /gene="tuf"
                     /locus_tag="PK224_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRB15059.1"
                     /translation="MAKAKFERRKPHVNIGTIGHVDHGKTTLTSALTKICADRGMAKF
                     ISYDEVAKASESQGRRDASKIMTIAISHVEYETDNRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADKVDDKELLELVELEVR
                     ELLTKYDFPGDKIPIVQGSALKAVEGDQGPLGVPSILKLLEAVDTYIPTPTRAIDKPF
                     LMPIEDVFTISGRGTVVTGRCEKGIVKVGDEIEIVGLRPTQTTIVTGVEMFRKVLDEG
                     QAGDNIGVLLRGTKKEDVERGMVLAKPKSITPHTKFKAEIYVLTKEEGGRHTPFFNGY
                     RPQFYFRTTDVTGIVTLTPGVEMVMPGDNVTVTGELISPIAMDQGLRFAVREGGKTVG
                     SGVVTEILA"
     gene            complement(24041..24116)
                     /locus_tag="PK224_04660"
     tRNA            complement(24041..24116)
                     /locus_tag="PK224_04660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24081..24083),aa:Thr,seq:ggt)
     gene            complement(24184..24258)
                     /locus_tag="PK224_04665"
     tRNA            complement(24184..24258)
                     /locus_tag="PK224_04665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24224..24226),aa:Gly,seq:tcc)
     gene            complement(24402..24487)
                     /locus_tag="PK224_04670"
     tRNA            complement(24402..24487)
                     /locus_tag="PK224_04670"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24451..24453),aa:Tyr,seq:gta)
     gene            complement(24725..25660)
                     /locus_tag="PK224_04675"
     CDS             complement(24725..25660)
                     /locus_tag="PK224_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15060.1"
                     /translation="MRPVLVYNGWFRAVLPTMAAGLVLGLLIFGQASAETAAPSSDSS
                     RLWGALMAEAKALHLPTRFLEQIPAQFVAFEFEDLHAFAAEYHPVEHRMVLDRSLSLN
                     AAGRTLRPLARLTHKELETLYHELFHAYMDFLEQERAPSAAHASFMAFAREQQRCRYQ
                     QVLITPVLQKKDLKEERFLSEAESWEALNETWAVFVGWAIWTQLEVERKGARELRSSG
                     RSLNVPAWIARLSQAEQDASLRGYYEPEDPSEKAMARKRFLAPDFRLSAPELLLLMKE
                     VLGSPANMTRQADQVLTRGRVVPASRGACEAPAAS"
     gene            complement(25727..27754)
                     /locus_tag="PK224_04680"
     CDS             complement(25727..27754)
                     /locus_tag="PK224_04680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRB15061.1"
                     /translation="MDRESKPYILKRTPDQDHVRKFSLDYAKELNAQQYAAVTAADGP
                     ALVIAGAGSGKTRTLVHRVAYLIDSGVDPSHILLLTFTRKSSEEMLERVGALIGSRSQ
                     RVCGGTFHSVANMLLRRHGRVLGIEPGFTIMDRGDAEDLIALLRAQLGLNEKDKRFPR
                     KGTIAEIYSKCENTLRGLEEIVLDEFSHFADHLEALGKLQRAYQAAKRQRQLLDYDDL
                     LVLLRDLLTKDEPTRRTISQQFRYILVDEYQDTNRLQAELIRKLAATHDNVMVVGDDS
                     QSIYAFRGATFRNIMEFPSWFPGAKIYKLEENYRSTQPILSLANEIIQEAPEKYTKHL
                     FTRTLDGPLPALVEAAGENAQSRFVAQKILELREEGVPLSEIAVLFRSSFHSFDLEIE
                     LSRCGLPFVKRGGIKFIEAAHVKDLLAHLRVVVNPQDAVSWHRVLMLVEGVGPKKAHD
                     LVAAMVRVNDPYQVLRDSSGRSGKQLKELALVLDSLSKSDDLSPTEQVNRVYEYYLPI
                     LKDHHDDYPKRIRDLDHLHTIAESYPSLAEFLADLALAPPDGSAVGVEPAGRDDEQVV
                     LSTIHSAKGLEWQCVFLLWVVDGKFPSVFSFNTDEELEEERRLLYVAVTRAKRYLFLT
                     YPINVYDRSSGMLLSKPSRFLDHVSPRLFETLALVEEGHGHEWGHEHDRYV"
     gene            27933..28769
                     /locus_tag="PK224_04685"
     CDS             27933..28769
                     /locus_tag="PK224_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15062.1"
                     /translation="MTTRPHSFAQQIQTVAELMLAVAGESVSVIKRAAPEQALKLKHQ
                     DEWTIYLEFLRVMFNLTDRVSALHIPLKEQPQFMDELTDTVIDQLKKTLEPAFGAGSD
                     QTEIVLTIGTAVAESRQTYERYRFLVTEDSPAKNDMYRDFSDRVARAIRVPRSPGVTA
                     AATLCVSAVIPALIGIFEGQAPPVAADATANPATAGGSSAPRGATGADIKLVSVMSSI
                     KGEEVETRWGLHPRFRQDLTQEEAKQLTTTMNRVAKILGERYATVAFSDQWASWHKAG
                     HA"
     gene            28839..30998
                     /gene="glgP"
                     /locus_tag="PK224_04690"
     CDS             28839..30998
                     /gene="glgP"
                     /locus_tag="PK224_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRB15063.1"
                     /translation="MQAPDHIQPPQDTVPQSLHRLGELARNLWWSWNQPARQLFESID
                     PTLWFLTHHNPVQLLSGVKPERFVTLAGDPNFVRQYSAVLRAFDEYLSNKNTWAATEF
                     SALQNSPIAYFSAEFGLHISIPIYSGGLGILAGDHCKEASDLGIPLVGIGFMYPQGYF
                     KQRINPEGWQEATYAPFNRYDSPIHQAMTPTGIPCSIKVEIGHRQVTVLVWQVRNGRM
                     SLYLIDTDVPENTPEDRALSARLYGGDQEIRLCQEFLLGIGGVRILRALGINPAVWHC
                     NEGHSAFLTVERIREFVTTGHSHAEASDLVRQSTVFTTHTPVPAGHDIFPFHLMDRYF
                     SNYWGQLGLSREEFLRLGETPENAGHGFNMTALAMRLSAHVNGVSREHGRVSRQMWQH
                     LWPGLAQDLVPIRSLTNGIHAPTWISPESNSLYAKYLSPDWAERVDDPTIWQRVTDLP
                     DDALWAVRQTTRRKLMRFIRERARDGWIRGHLQPMQAIARGTLLDPEALTIGFARRFA
                     TYKRATLLFRDLDRLKQLLHNRWRPVQIVFAGKAHPADEPGRYFIHEVLSFCNDHKLG
                     GHVAFLEDYDMHMAKYLVQGVDIWLNTPRAPLEASGTSGQKAALNGVINLSVLDGWWQ
                     EGYNGANGWGIQPLPEGSDTQAQDAHDAEQLFRLLEQEVVPLFYQRDLDGIPRGWLQI
                     VKESIKTVAPRFCTKRMVKEYMRQLYAPATARTPNKW"
     gene            31030..32406
                     /locus_tag="PK224_04695"
     CDS             31030..32406
                     /locus_tag="PK224_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB15064.1"
                     /translation="MRRHTTWVLPVGLALLAWAIPHSATAVPSATKPAVTPVERQAMT
                     LYQAAEYSRVLELLQQIPAGQQPALELLRYGILSHLKLGKPEEAWKLYTKLVADNRPD
                     EPAVLRDIARSFIVTRVRDTQEHIRIAAYTALAEMGESDTLPILEDGLLDSSALVRAR
                     AAEAIGRAGLAGRSSALKRALRDEAPGVRIAAINALSDAKVAGITDQLTEIARVDEGP
                     ESIFAFAGLYKLGRTDVLTDISNAVTLPDPEVRMAALGMLGRLRRPASLSILSQAVYD
                     PHPAVRAFAAGALGEFGSTDGIAPLTHALSDENPRVRSVAASSLGRLGATETRSVVQP
                     LLRDPDEHVRISAVEALLRLGDASAVLNAADLARHPDPSVRGGAAHALAAATTPNALT
                     VLESLLRDQQPLPRLMAARALGKLQQKSLISPLKTGLQDSDPTVRIASAGSLIHILSR
                     KDKQGSAR"
     gene            32422..32856
                     /locus_tag="PK224_04700"
     CDS             32422..32856
                     /locus_tag="PK224_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15065.1"
                     /translation="MLRFIGLLMLLALSFGFGYLWGKQPANNLEQKVRDFSRNVVDTT
                     LGVERDLHRRQSLVDAKSKVVQAKAELYNKNTTNAAKELAEAIDSLELATRGGKQVDP
                     NAQVKELAGKIRELRLELSLGKKVSATKLDDVQKELDLLLRK"
     gene            complement(32865..33695)
                     /locus_tag="PK224_04705"
     CDS             complement(32865..33695)
                     /locus_tag="PK224_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB15066.1"
                     /translation="MEHTGEKEGQAQAVSPISQVEDETLTDQVSDQLAASLDSSAGAQ
                     DLTDPLQEVVLEEEQVKDEIDIQIDLLKDPDWVVRREAAITLGEMGDERCVEPLAAAL
                     RDGDWQVREVAIEGIGQIGSPAVEVLLKLLRDWDVRKSAIMALGKIRDERVLEPLMQQ
                     LRNDEFMEDATDALVNLGEPALPGLIKALKDKEELVRKQAVIALGRIKSPEAIDPLIE
                     MLQNKDWFTRLTAAAALEAIGDERGREAIKPLLKDSDMVVKMRVERILAKWKKQPAAV
                     "
     gene            complement(33760..33963)
                     /locus_tag="PK224_04710"
     CDS             complement(33760..33963)
                     /locus_tag="PK224_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15067.1"
                     /translation="MAKKPVGESDEVRLKKKIAVRVTKEAKLSGDPAARSLHKRLKRV
                     QRKRRALALRKKHAAGNQTEAKS"
     gene            complement(34091..34750)
                     /locus_tag="PK224_04715"
     CDS             complement(34091..34750)
                     /locus_tag="PK224_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRB15068.1"
                     /translation="MDMNSRNPFRRKAGYAVIPLAGWLLLSACSALPKGELTLDLGIK
                     DRGVASWYGKEFHGKLAANGEAFDMTAYTAAHRKLPLGSVVRVVNLTNGKTVQVRIND
                     RGPYVAGRMLDLSHAAARELGMVEAGTTAVQIEVIGDHRPVAQIPSTKIPAVSGMVLN
                     TDMRAVRQHRRPEEGVERPAVPVRMMPQEALYVRRERRIGSMLAADHTAHNTVPVLIL
                     S"
     gene            complement(35103..35762)
                     /locus_tag="PK224_04720"
     CDS             complement(35103..35762)
                     /locus_tag="PK224_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15069.1"
                     /translation="MIRQHVVRGTSAPVAVGLLLLLGCSGKGDIIPMNLTPKMQTDTT
                     SAVQVVKPMAGPRVMVIPFEDGRPDRTKVGSRSSMWGGDTYFNVSSGSAGEETAQAFA
                     DYLKRKGWQAQYAKTAPAASEAGPDVVLSGKILELAVDARRGVFLTDIEARTKLVIQA
                     SNREDNSSLVRTDAHSGSHDNVLWFEPQDGESILSEVLEKNFERFVVNTKFEDRGIRF
                     R"
     gene            complement(35819..36196)
                     /locus_tag="PK224_04725"
     CDS             complement(35819..36196)
                     /locus_tag="PK224_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15070.1"
                     /translation="MRSDINQPRPSLLGVLVIGAALCVALAGLGSFLTSVYAASNTVH
                     EIKGTVEAVNLADDPPVIVVRGQHGANEATVVGAVLKQGATIVRGKKRIGLDQIHAGD
                     KVTLKYVKTRDGLAVRSIVLHRN"
     gene            36445..37413
                     /locus_tag="PK224_04730"
     CDS             36445..37413
                     /locus_tag="PK224_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB15071.1"
                     /translation="MIEVRNITKRYGNLTAIDRVTFRVDKGEVLAFLGPNGAGKTTTM
                     RILTSFIPATEGTAQVAGFDCAEQPDEVKKRIGYLPETPPVYQELTVSEYLAFVGQLR
                     GLSGAELTAGMDRVMERLSLGSVRQRLIANLSRGYRQRVGLAQALIHDPPVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSHSVILSTHILPEATAVCQRVVIINGGRIVAEDTPDQL
                     SARLRRSEKISVTLKTPPADVLERFREVAGVEHVLTTSEPHTVLIECALGRDIREEVA
                     RFAVGQHWGLLEMKPVSMTLEDVFLKLTQREDGLPKADDASESELH"
     gene            37388..38188
                     /locus_tag="PK224_04735"
     CDS             37388..38188
                     /locus_tag="PK224_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRB15072.1"
                     /translation="MQAKVNYTSPMPPVQAIIAKELRSYFVSPIVYVVGAVFLLTFGF
                     LAYLYIVFTGAQAIQLMQMQGGTAQINLNDLVFRNLFASMRFVLLLILPILTMRLLAE
                     ERKLRTFEFLMTAPVRVSEIIVGKFISVYLVFLGMLLLTTLVPLTLALFSDFDWYPVL
                     TGYLGMTLLGGFFLAVGLFASSITENQIVAAFTSFGLLLMCWLLAGLGSLLGDTPAGH
                     VVSYLSFMEHFDHLVRGLVDTKDLVYYVSGTVLMLFLAHRVVDSSRWK"
     gene            38179..39708
                     /locus_tag="PK224_04740"
     CDS             38179..39708
                     /locus_tag="PK224_04740"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRB15073.1"
                     /translation="MEITRRPLPIGAIGTGLAIAGVITYTLVPDKLWLVTLLEGLALV
                     LLIWAFAIHFERLKTFTSQRSTRMGANSALMVALFLGILAIVNFLAARHSIRWDFSEN
                     QNYTLAPETYRVLRNLTRDISITVFTREKDPGYQAYKERFDSYRQASSKLTVQFIDPE
                     RQPKVAQNYGITRTDIAIFESGPQSVRITSPAEVEITGALVRVSKDTKKRVVFLEGHG
                     ERSLDDTDRGGLTLTKEVLQKQGYDIGTLTLLQESAVPEKTDVLIIAGPRRALTKEEK
                     ERIQAYVVKGGHVLVMVDPDTQSNMDDLLKTWGLELGPGVLVDLQDRLAQGDLTALLV
                     RTFTDHEITHELTAAVLFPLARHLTFQEESGKEWDYVPLARTSPRSWAETDMKGRVVS
                     YTEKEDVQGPLALAAALTPKQAPEEGKPRPAVVVVGNSAFASNGFINFVGNSDFFQRT
                     VGWLSEERDLISLTPKDLAVRPFTPNPLQERILLYVQVILLPAMTILCGLLVWRKRRR
                     L"
     gene            39713..41083
                     /locus_tag="PK224_04745"
     CDS             39713..41083
                     /locus_tag="PK224_04745"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRB15074.1"
                     /translation="MARYWSTLILAGVLAGLGLYLYLVELPDQRTEVATATQAKQILP
                     FTEAQITSLTVRSQSSEVVLTHTPGQPWTITAPLQTDADQRQVQALIRALVMGKVSRL
                     VETHPASLAPFGLDHPSTVVTLTAGDKQETLSIGDAGPLSSTLYVLRTSDEAVLLTDL
                     APKDFLNRTLLSFRRKELLQVNQQETERLRLTYPTTEIVLYALDEKPKKKWKIRYPIE
                     AEGDRTEILALLFRLEDLKALSIVDQGPEREKLAPMLTKPKIKVTLSAAGVDHVVRLY
                     QPDPASGEAYAQTTPDGPIYKISPSAIKDLTKDLFTLQDKRLLGVDTGDIAMLSIKTR
                     EEQYVLIHDRNEWILEDQPTEKLRQDVADLLVSRIVNLPAEERVLKQAGPLAPYGLVA
                     PVAEFVATGKDGRVAGRLAMGNQVGGLVYAIGHRIPGIFQVRPDLLKQIPSRLALLGQ
                     GPGSPP"
     gene            41165..41575
                     /locus_tag="PK224_04750"
     CDS             41165..41575
                     /locus_tag="PK224_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15075.1"
                     /translation="MTNSRSHGAVVLSLSLLLLSACATEETMTGGGSTPSSSAAMKAS
                     AQAYDSLQSCLARIQSGGTAGTRQLAEEGCQRDEALRQSIAGNATAKSGNRAAAGAAG
                     DSLEACMARIPKDGSVGQRMLAEESCQRDQANRR"
     gene            41686..41762
                     /locus_tag="PK224_04755"
     tRNA            41686..41762
                     /locus_tag="PK224_04755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41720..41722,aa:Arg,seq:cct)
     gene            42194..42325
                     /locus_tag="PK224_04760"
     CDS             42194..42325
                     /locus_tag="PK224_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15076.1"
                     /translation="MIEFPLILIGYALLPLIAIQLMIMADRPQKTGEQNAFPQVEIL"
     gene            42636..43325
                     /locus_tag="PK224_04765"
     CDS             42636..43325
                     /locus_tag="PK224_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15077.1"
                     /translation="MKPANLFICLSLLFLATAGCHGYAFIETPTTDGFHSKLPSPYTR
                     AVVWGGRPDTVQSASTWLLKKGVLVVDQTKVLQAAADQKVSLTGYQYLETDVLRMAKL
                     VGARLVVFSNAEVGSWEALDWSSGFPHSQRVYSATVALRAVDVNTGEIEWSGKAQSTE
                     RFSNIEEGVSLLTCHALATAWGLRNPGAVAPESICPPGSGLMSTEMSPPKAHAAPASS
                     GRENLTPSMNY"
     gene            43529..44332
                     /locus_tag="PK224_04770"
     CDS             43529..44332
                     /locus_tag="PK224_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15078.1"
                     /translation="MASTTHAAENRPFWTEQALFHFGEDVFFTGRASCAPNIEDGRQR
                     AYVAAVQEVKNFTRTQEVGGFPIDTQMIFEDLHPADCAQGLITVWRLLRVPRTALESL
                     SRHSSPGKQKDISPIQAQITAIRNLTPRVGMLRDEIWNRYGLPRSIWARPEQGELIWD
                     YSQFGLIIVFNQDDVLTRWRLNGPHPRSSEDEPILSRQDNPTGKDLPAIDLTVRLQQL
                     EEQQDLDRRRQAQRYCTIRFADVPESLRPKHGGCEQREYERLKEQHPPF"
     gene            44370..44864
                     /locus_tag="PK224_04775"
     CDS             44370..44864
                     /locus_tag="PK224_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15079.1"
                     /translation="MAPGQKQPSTILIVDDDPSALLVSTKPLRDAGYTVLQAPGSAEA
                     LRLYAEHPSPIHLVIADVFLPPPGFQLSVDRNPYPRVNGLDMVERLLQDKREIRVLLM
                     SSSPSSELQNRGLVRAGLPFLKKPFTSETLLSLVREVLAGPPAAPDQKNATRPGNGDV
                     EWVD"
     gene            44972..46684
                     /locus_tag="PK224_04780"
     CDS             44972..46684
                     /locus_tag="PK224_04780"
                     /inference="COORDINATES: protein motif:HMM:NF037406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15080.1"
                     /translation="MARARASEPQRSRSKQNAVEELNRGITTLRELSSQIDDLSRDGF
                     PYRDAVRARTELSLRESIRRLFGEKSPEYQAHKNHKLRIGNRTESAQSTTLLKELIVQ
                     LENQKAELLGLTPAETAPPPPTAPERPTLTAVPPTQPAIPVVPPQAPSTAPLATVSMI
                     NAPATVSVATTSISQPAQPIAPIVMTTPVSSTSASPPVTQGTASQPIRDTSIQRLPPQ
                     PPSPPLHSATSEAPGIPAEKTAPASLPASPSLPAEPVVAAPSTNSATPSEQTVRGEAA
                     PTPLPTMAPARSTIPSGTIPPIPRMNSVPAPVPTAPIEPPPTPQQASKPTDIPAQKPA
                     PAPVAVIPASQATTEIAAEKQARDPRPPVPQVRTEISLPPAMPALASVLESKPEPPVI
                     SPVKPPETPTVLTTPLDTNRNNHQSEGATLDTLRRMCARFHLVARQLRLRKEYRPTLE
                     ITDEYDLQDLFYALLRLQFDEVGTEEWAPPYADGARRTSYLLDWEKTVVVVKQTRSGL
                     TSRDIAEQIATDKAHYSGRPNGATLLCFIYDPDGRVGNPRGLEADLSTVGDTYRVEII
                     VAPK"
     gene            46811..47065
                     /locus_tag="PK224_04785"
     CDS             46811..47065
                     /locus_tag="PK224_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRB15081.1"
                     /translation="MPDRAYVLINVHPGQTADVVKALGDIKEIKQIDPCWGKPDIFTI
                     VEIPHQDALTQLVLAKIHAIPGVDQTDTHMVYRLQEGKPK"
     gene            47062..47508
                     /locus_tag="PK224_04790"
     CDS             47062..47508
                     /locus_tag="PK224_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15082.1"
                     /translation="MRTRVSGIATFVLVSALAGCAGGPPAPEPDVMEVILPVPAEQVK
                     TALSDVLTQGGYTVDQDDTGNITTGMRQEIRSPWNGLLRWRFGVGKTRVEARVVPQDD
                     STTRLRLQVFHRGKDGIFDSWEDAETPLPQSAANEIRLLKNALRLL"
     gene            complement(47516..48910)
                     /locus_tag="PK224_04795"
     CDS             complement(47516..48910)
                     /locus_tag="PK224_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB15083.1"
                     /translation="MSQAKLFVVDDDEAARTLLAEALVKEGYEVEAFSSGQAAVERGK
                     QSRADVVLTDIRMEQGDGFLVLKEFKKFSPDTSIVLLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKRDEIRLVVQRSLEHCRLVRENARFREDARAREPWSQLVGSSPAMLEVYKLVAR
                     VSEGRSTVLIEGESGTGKELIARAVHLNSPRRDKPFIPVNCGALPDHLLESEMFGYEK
                     GAFTGAVGAKAGLFEAADSGTLFLDEIGDLGQALQVKLLRVMQEQEVRRVGSTASVKV
                     DVRIIAATNRDLATLVKEGKFRDDLYYRLNVVRIVLPSLAERREDITMLAHHFLQKYA
                     KQSLHVRGFLPETMALLKRYHWPGNVRELENAVERAVSLSHGPLLLPEDLPESIRAEP
                     DSGELLKGQPAEGDQDALLTLDEVEKRHLARVLKETRGNKVKAAKILGIDRRTLYRMA
                     ERFGLDFGDEAEEK"
     gene            complement(48932..50083)
                     /locus_tag="PK224_04800"
     CDS             complement(48932..50083)
                     /locus_tag="PK224_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15084.1"
                     /translation="MKSRNHVVLVIAAILFLIVMVMEWMFPLNVVGAFGFVLPILLVA
                     TVRNRRLMIVTLALCILVTFVGLFQPGKKRERFTAVVFNRIMVACVLTGVAYIGVTWE
                     ERKAREEAAKAALATQTEHLLLANTQLVEIKDKLARSERLAAVGQLVASVAHEVGTPL
                     HSIAWHVEALAEEPGVTPAMQKRIGIIDEQLNRVVRIIQDLLSSTRQRKPEPTWYPAE
                     RLIEPVVALMEPGYHAKGVTLHTEVAPALVVWADAEKIHQVLVNLLSNALAATGAGGQ
                     VTVAVTIRQAFSEEMESASCTGRSNLSTVVTLVVSDSGCGMPREDVERAFQPFFTTKA
                     VGKGTGLGLFLSREAVSAHGGRLTLESEIGKGTTVTVVLPGLPSDSAAV"
     gene            complement(50114..52375)
                     /gene="malQ"
                     /locus_tag="PK224_04805"
     CDS             complement(50114..52375)
                     /gene="malQ"
                     /locus_tag="PK224_04805"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRB15085.1"
                     /translation="MYDGTEQDLLRLLASRVGISPEYHDIAGTLHVTSDETRRAILSA
                     MGFQVGSGAVLSQELTALDEAPWQQPCDPILILQQGYGPTQWTFRPALEEGEEQHVAV
                     AWSLTDEKGTIVHREETSPGLNPCEVRSLAGRRMVRVELPLVQNLVLGYYDLSVGTMG
                     SKVVTRGTLRVVVAPPHCFVPEELARGGRVWGLAVQLYSLRSQTNWGVGDFTDLSRLV
                     EWAGKELGAGIIGLNPLHALKNSRPHHLSPYSPTSRLFLNELYIDLDRLPEYHASPDA
                     QRLRNSAEFQKELEQARAADLVDSESVIKAKRMMLDLAYRQFLKNNYTGIEPSLEPTT
                     ARGWLLHRFIRDEGESLERFALFQVLEEERRLIEQSPKLWPEWPAQYRAPGSPALREF
                     SRRHRKRVRFFQYMQWVAADQLRAANTRAAQVHMTVGLYPDLALGSDRNGAEAWMLQD
                     VLALGADCGAPPDAFAPQGQNWGFAPFHPLRLKSTGYRSFIELLRKTLRQGGAIRIDH
                     VMTLFRLFWVPRGMSAEAGAYVQYPAEDLLRILALESLRAQTLVIGEDLGTVPDYVRE
                     QLARYKILSYRVFYFERNWDGSCKSPSAYPEQSLAVVTTHDLPTLTGYWTGEDIRLRA
                     RLGMYANEQAVQQAFEERTRDRGHILRVLNGAGLLPSGVSDQPDSVPVMTSALCRAIH
                     VYLATSPAWVVMANLDDVIGEVTQMNLPGTVDAYPNWSRKLSLSLEELQRDERVQALA
                     AAVCTLRPPAATA"
     gene            52674..53405
                     /locus_tag="PK224_04810"
     CDS             52674..53405
                     /locus_tag="PK224_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15086.1"
                     /translation="MTPQLSHSGSGTLLAIAAQCLVFSATAQALPEHSRVDVTGDYRY
                     AYHEPETASEARQHACTEALYQAISTSAAVREQTASIVDSKLFRNLVHTLASKHVTDQ
                     QIIQQSETGRTVYCKVKAVFHADEVERVLIAQTTGGTEPGLDQNRALKIVSAREEADG
                     TLVIVYQALKRLDWLGTAYQGSLRESADVMVDFYDDQGVLVRSSRHPARKTATGDDVM
                     NPGEVGTLRIAKPLNAKTYRVWVVK"
     gene            complement(53491..54318)
                     /locus_tag="PK224_04815"
     CDS             complement(53491..54318)
                     /locus_tag="PK224_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRB15087.1"
                     /translation="MRHGAWSMLGAVGVLLLTSGCVAIEAGHEGVMVEQPFFFGHGGV
                     DPAPSKTGRVWVAPTTKVIEVDVRPLQYSEHFDIISAENAPVSFDAFMIANVVEGRSP
                     EIVGRYGTNWYQNNVKEAFRTFVREEVQKYPLFQLTTDPTTRTKLQDAIAREVQTKLI
                     DKQNLPIRLNRVVVGSILPPKGVVEQTTQTIVQEQRKITMVEFQKAEEAREKAEKQRG
                     IADRAYRESLGLTAPEFVDLRRIEVQKEIVQHSPSALTVIMGLERVGINMPPLASDR"
     gene            complement(54508..54900)
                     /locus_tag="PK224_04820"
     CDS             complement(54508..54900)
                     /locus_tag="PK224_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB15088.1"
                     /translation="MKVWVMGVGFAVMVAALAACESNATNQDAAKPAGGATPAEVQVG
                     EAKFTANCAACHGVRGVGTKQGPPLVHKIYEPNHHADMAFQRAAENGVRAHHWEFGNM
                     PKIEGVTSGDVEHIIRYVRWLQREAGIY"
     gene            complement(54927..55103)
                     /locus_tag="PK224_04825"
     CDS             complement(54927..55103)
                     /locus_tag="PK224_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15089.1"
                     /translation="MTPEEFFMYLIIALVIVTPIAARLYRRLTLNRTTQMLRDQLQQS
                     QGPRTTTPPSEPPR"
     gene            complement(55200..55898)
                     /gene="queC"
                     /locus_tag="PK224_04830"
     CDS             complement(55200..55898)
                     /gene="queC"
                     /locus_tag="PK224_04830"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRB15090.1"
                     /translation="MTHVDSKAVILLSGGLDSTVTAAIANQDGYRLYCLTISYGQRHR
                     VEVERARAVAQALSAAAHVVVDVDLRAFGGSALTDDVSVPKDRTQEERAGGVPVTYVP
                     ARNTIFLSLALAYAETLEAQRIYFGANVLDYSGYPDCRPDFIRAFEVVARLGTKMGIE
                     GRAVEVCAPLLMLSKAEIVQRGQELRAPLELTHSCYDPGADGVACGRCDSCRIRREGF
                     RLAGVVDPVPYAIL"
     gene            complement(56019..56594)
                     /locus_tag="PK224_04835"
     CDS             complement(56019..56594)
                     /locus_tag="PK224_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HRB15091.1"
                     /translation="MATKVHAKKKTPTTKAKAPAPEKPVKKERPAPVTAMTEKDEDSV
                     AARVVAALTLKETPKEREERQRRREVLQRMLLSKRQEIMREIEGNLGQSLTEDQQRRL
                     ESARDVGDQALMDLDRELGISLMEMRNRKRQAIDEALTRLSEGTYGICAECGIEVSEK
                     RLEAVPFAKLCVQCQSRQELLEKIEKEEDRD"
     gene            complement(56700..57215)
                     /locus_tag="PK224_04840"
     CDS             complement(56700..57215)
                     /locus_tag="PK224_04840"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRB15092.1"
                     /translation="MNYKALIREVPDFPKPGILFYDITTLLKNAQAFRSIADELAARY
                     EGQRIAKIIGIESRGFIMGGTLAARLGAGFVPVRKPGKLPADIYEVEYNLEYGSSVLA
                     IHRDAVSPGERVLIVDDLLATGGTAAATIDLVRQLGGEIAGLDFLIELKGLNGRDRLA
                     GYDVHSMIIYP"
     gene            complement(57240..58238)
                     /locus_tag="PK224_04845"
     CDS             complement(57240..58238)
                     /locus_tag="PK224_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB15093.1"
                     /translation="MARRAAVRQDNAVNSPVSARRPRVARGDEDEDAESGSHAEAEES
                     GADNRPAESGRAEGLDTIKSYLREVRKSILLNFKQEQALGKRVMEGDEAARQEMIEAN
                     LRLVISIGKRYMNRGFPFADIVEEGNLGLIKAVEKFNYKRGFRFSTYASWWIRQFIER
                     AIINQGKLVRLPVHVVERLNRYLGKVEQLVHELGREPRADEVAAKLKTSVAEVDDLKQ
                     LVRTTCSLDSPISDRQDTFLRDVIEDPLCLTPAETTEGVMRRAELMAWVKELPEKERT
                     VILARFGLDGAESRTLEEIGQEMGLTRERVRQIETAALARLRGIIERKTMKRADLL"
     gene            complement(58253..58600)
                     /locus_tag="PK224_04850"
     CDS             complement(58253..58600)
                     /locus_tag="PK224_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRB15094.1"
                     /translation="MPGAVLALRLVFDQWEGCAAMTDTGESVRARMLVSGRVQGVGYR
                     AFTCRMAVARGLTGHVENLDSGQVAVDAEGNRGVLEEFLSDLKKGPVGSRVTQVQVEW
                     SRATGRSQDFMIK"
     gene            complement(58914..58990)
                     /locus_tag="PK224_04855"
     tRNA            complement(58914..58990)
                     /locus_tag="PK224_04855"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58954..58956),aa:Pro,seq:cgg)
     gene            complement(59026..60129)
                     /gene="ald"
                     /locus_tag="PK224_04860"
     CDS             complement(59026..60129)
                     /gene="ald"
                     /locus_tag="PK224_04860"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRB15095.1"
                     /translation="MIIGVPKEIKDYEYRVSLTPDGARVLLQAGHRVVVEPSAGQGSG
                     FSDDAYRQAGVQVAGSKAEVFKQADLIVKVKEPQLSECPLFRPGQVLFTYLHLASLPD
                     LTKALVAADITAIAYETVEARDHSLPMLRPMSEIAGRLAVQIGAHYLGTVQGGRGLLL
                     AGVPGVPPAHVVVLGAGVVGTSAVRIAVGMGARVTVINLDLDRLRFLDDLYGGRIATC
                     AATESAIERAVVEADLVIGAVLVPGARAPKVVSRALVKKMKPGSVIVDVAVDQGGCCE
                     TTRPTTHSDPVYVVDGVLHYCVTNMPGIVPHTSTLALTNTTLPYIVRLASEGVEKAIR
                     SDPGLAKGVNVMNGRITCQGVADAHGLRFTPVM"
     gene            60292..60660
                     /locus_tag="PK224_04865"
     CDS             60292..60660
                     /locus_tag="PK224_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15096.1"
                     /translation="MDAGSCNACSAAGAPLMKFSLGKDFFGRTYDRLSPASDQSPKWY
                     CEPCSMMKNLQRDFRDIRAEFDKLAKGQASALSEPEAKQRAQLRLNEIAAIAGTPAAG
                     SSLLSSTDVKQLIEQFHARV"
     gene            60699..61049
                     /locus_tag="PK224_04870"
     CDS             60699..61049
                     /locus_tag="PK224_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRB15097.1"
                     /translation="MTGYQRHIFVCTNKREPDDARGSCSKLGSDALHACFKQEAKRLN
                     LKGVVRANKAGCLDYCAQGPTVVVYPEGIWYRVTSEVDVKEIMERHIVKGEVVERLLM
                     PDQSPLPLLSPLKA"
     gene            61085..61498
                     /locus_tag="PK224_04875"
     CDS             61085..61498
                     /locus_tag="PK224_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15098.1"
                     /translation="MPADDKWKANMEKVAFMKAFPGLLRHWEALAGKTVEAVAPLKSK
                     AGAAALICTDGSFVVLPPLTTEPYELGEALQAGRSYLEPKHPEAYIGYDQLLKKDKDA
                     QRTARLENILGAIRNNMEQIPELKDRLKDLVKEWK"
     gene            61564..61791
                     /locus_tag="PK224_04880"
     CDS             61564..61791
                     /locus_tag="PK224_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15099.1"
                     /translation="MLVIRDHLVRHPDRCTHQAICVPICPTSAWLSTPPYKFDPSRCL
                     ESCRLCLDACPSQAIYAVFKKGEKLLEPQKK"
     gene            complement(61932..62303)
                     /locus_tag="PK224_04885"
     CDS             complement(61932..62303)
                     /locus_tag="PK224_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15100.1"
                     /translation="MIRLLGVMYGVVATWFAPAMVLAVLALLGLLLNPRVIGLRHLLV
                     EVATVSFQFGCAALASVGLWRLRRWGLGVACAYNLVWAGVLSRHALESGSSLSFQDTM
                     PLALVLLAVFLLLPAVRNATF"
     gene            complement(62374..63375)
                     /locus_tag="PK224_04890"
     CDS             complement(62374..63375)
                     /locus_tag="PK224_04890"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRB15101.1"
                     /translation="MLKQVSRSFYLTLNVLPATVRDQMGLAYLFARAADTIADTDLIG
                     RELRLRYLNQFRTQFSSGPIARRDVHAIQAALIPHQADSAERVLLQRLEDCLTLYEQF
                     DPADQERIRWLMGVLPDGMTMDLMHFQGDSAQDLTAFQTLDELDQYTYYVAGCVGEFW
                     TRMVCAHCPSMSGWDVKKMSAIGVRFGKGLQLTNIVKDLARDLHRGRCYVPESLLREA
                     GLTSVDLLNRENLPKFRPVLMRLIKMAIEHLDQGWMYTMALPRLEIRQRLACMWPILL
                     AGETLKRVAAAPDLLDPSVNVKAPRSVVYRVMALTTFTGGCGYVGTAYWGRLRKQIV"
     gene            complement(63427..63873)
                     /locus_tag="PK224_04895"
     CDS             complement(63427..63873)
                     /locus_tag="PK224_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRB15102.1"
                     /translation="MSRVSGFPQLGFVCVLMCGTVIAPTLVHAADQAPQVPPLQVPVD
                     QLDGIQRATLILDSYSYTPSHVIVQAGKPVELLLTSITSITPHNFLLKNEAAGLSIER
                     DVSSGRTVTVQFTVRKPGLYPFYCDKKLLFFPSHREKGMEGVLEVR"
     gene            64089..65006
                     /gene="htpX"
                     /locus_tag="PK224_04900"
     CDS             64089..65006
                     /gene="htpX"
                     /locus_tag="PK224_04900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRB15103.1"
                     /translation="MKSLKGIGLLLISNILIYLTLSITVRVLVNVVLPAFGIDVRGAF
                     SQELLVWSLVIGFGGAFISLLFSKQMARAMLDCVQITQPRTNAEQVIYGSVREIAERL
                     HITMPEVWVYESPDPNAFATGPSKNNAMVAVSTGLLANLREDEVKAVLAHEMGHVFNG
                     DMFSTTVLAGLMNTFVHYISNFVYQMVAQPQGGDREEGQSGSPILGFVVYIFLQVVLS
                     VLAMMVVSWHSRHREYAADAFSAKVYGKESMIKALQAIDRWVNRAQFEYSTQDALATM
                     KISGNTSGFLHLLATHPPIEERVAALERL"
     gene            65022..65645
                     /locus_tag="PK224_04905"
     CDS             65022..65645
                     /locus_tag="PK224_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRB15104.1"
                     /translation="MPISRPTCSWKVLVLGLAVGLGLGALEMGLGSVDAAEQGATRMA
                     AKASTVYDFTLNDIDGKPVSLGQYKGKVIMLVNTASFCGNTPQYSDLEKMYETYKDKG
                     FEILAFPANNFGQQEPGTNEEIKGFCLTKYSVGFPLFSKISVKGSDKHPLYRYLTEQS
                     PFPGEVEWNFQKYLVDRSGNVVARYHHRTKPVADEVVKDVERFLAKN"
     gene            complement(65650..65925)
                     /locus_tag="PK224_04910"
     CDS             complement(65650..65925)
                     /locus_tag="PK224_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15105.1"
                     /translation="MKETDAEKLALLYERFCDVCLVEKEVWTEIYMPRTFKDGTAVRT
                     NLQDKYDVIIDDQTVEDALEANIPLGKAALSAAIQEYRTHVTFVKKA"
     gene            complement(66005..66691)
                     /locus_tag="PK224_04915"
     CDS             complement(66005..66691)
                     /locus_tag="PK224_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15106.1"
                     /translation="MDLPISAACGEPAMKMNGRWVICRLAVMVGVMAGVGFSVAVVEG
                     RDLMVPKEQATTEFEPTETPSMDVQEKGVPQSLFTWIKMARGYEVEWDTFGRKGWYTQ
                     DPRLKPIAPGTTLFSPDTPAVYIVFEVAPLEDPAQFAAQVFPVGADGKTGARAIVTDS
                     LEVPGHERYGFLELKKPSEQWPQGKYLVKIYITSLGQQPFHAVNQVGTMRFTVTDQPP
                     APAPSDSTTR"
     gene            66841..68271
                     /locus_tag="PK224_04920"
     CDS             66841..68271
                     /locus_tag="PK224_04920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB15107.1"
                     /translation="MNQPAPQQQTSLPLHPLEPSGDLTQSSDQNAKAESRRYGVRDAA
                     FQAAAQGGGENYFSAFALFLHASPFHIGILSALPQLVGVVAQLLSVKLLQYLRMPAQL
                     LIGGGWAQALCWLPILSLPLLLPEHGPWLLIGCAMLYFAFGHATAPVWNSLLVDVVET
                     SSRGAYFAQRARVTALTSFVTLGIAGTVLTLGQKWELSWIGFLFIFLGAAAARVGATR
                     CQTRISKLVPVHHIDAPHGFRHFLAQRATPDFRHFLLFSGLMHFSVLISGPFFVVYLL
                     RDLHWSYLQYAGWMASSISAQFFTLAPWGQIGDRYGNKTLLMVTGLAVPLLPMGYLLS
                     EHYLFLLSLNFFGGVIWAGLSLGLQNYVFDSLRPQERTRGVALANATNAIGWGIGALT
                     GSWLATILPAQVSLGSWALTPASNLPFLFCLSGALRLLIALSLLRTFGEPREIGTPPQ
                     RHLMWELPLVKPLVALLPWRNSRVAP"
     gene            complement(68299..68511)
                     /locus_tag="PK224_04925"
     CDS             complement(68299..68511)
                     /locus_tag="PK224_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15108.1"
                     /translation="MEYRSGRVGAGMRVIGLLGGLAAAGSLVTGCASEKPKPMAQPSL
                     EQVKGNADRGFDKLKQEEREHKPAGM"
     gene            68685..69134
                     /locus_tag="PK224_04930"
     CDS             68685..69134
                     /locus_tag="PK224_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRB15109.1"
                     /translation="MSLHDRLTEDLKSAMKSRDQLRMDVIRMIKAAVQYKEVELKQDL
                     DDAAMSRIMTTLIKQRKEAAEQFEKGNRQDLATKERQEISIIESYLPAALSPDELARI
                     VDAAVKESGAASLKDMGQVMKAVMAKLAGQSVDGKVVSDLVKAALQR"
     gene            69300..69947
                     /locus_tag="PK224_04935"
     CDS             69300..69947
                     /locus_tag="PK224_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15110.1"
                     /translation="MGILIVDDSPDQQLLLKAVLNKAGHLDLLIADSATAAYDQLGID
                     QPEPPQHTPPIDLILMDFLMPGIDGVAATQRIKSLESLRDIPVIVVTAKTALGDLQAA
                     FAAGAMDYITKPVNSVELLARVNSALMLKKEMDCRKTREMELRRSNAELQQALREVKV
                     LKGLVPICASCKKIRNDQGFWQQLEEYIQQHSEAEFSHGLCTPCIKKHYPGVYPD"
     gene            69992..70996
                     /locus_tag="PK224_04940"
     CDS             69992..70996
                     /locus_tag="PK224_04940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRB15111.1"
                     /translation="MGILIVDDSTDDRLLIQSILANAGYEETFVAESAAAAFRLLGLD
                     GPRQMAGRVDLILMDIVMPATNGIEACRQIKAVERYRDIPIIMVTVKTDPVDLQLAFA
                     AGAIDYVAKPISKIELLTRVRCVLRLVHEIERRRAREQELLEVMRQLQEANQMLLRLS
                     CLDGLTGITNRRQFDDFLDQEWRRAARESTPLSLIMLDIDYFKTYNDSHGHQAGDECL
                     RQVAQLISSHVNRPGDLVARYGGDEFVIVLPGTTVDGAAQVADTLRRKVCEAEMKHPN
                     GDPVTISLGFACTVPSRVAAPTDLIRAADQALYQAKQDGRNRAKQAGVLTLVHHTHKD
                     "
     gene            71079..72848
                     /locus_tag="PK224_04945"
     CDS             71079..72848
                     /locus_tag="PK224_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15112.1"
                     /translation="MAKRITQTRRTTSTSATRLPRTASRPSTALQREQAARLRAERAL
                     QRVSKELQRRVEELQRAKDAADVANRAKSEFLASMSHEIRTPMNAIIGMADLLWETTL
                     TPEQRKYLRIFRRAGASLLSLINDILDLSKVESGRLDLDSIDFDLSELIDKTSEMLAL
                     RANEKGLELVCHLANDVPCHLIGDPNRLYQILLNLLGNAIKFTEKGSVVMSITNDPEL
                     RTPGAIRFSISDTGIGVPHDKIKAIFDTFTQAHSTIARQYGGTGLGLSISQQLAALMG
                     GRIWAESTLGEGSTFHCVVQLGVQTAPPPSKVSGTMNLAGLRCLVVDDYPTNRLILRE
                     TLFGWGATVVEADSGEQAMEALEVSLKSDAPFTLLLLDCRMPGMDGFEVIERIHADPR
                     LSNLTIIMLASDRWADDIAKTYDLGLGGYLVKPIRRSDLQQTISIALGRSKSGFSPSP
                     QGAAPQAAPQGRSLRILLVEDSPDNQVLIQSYLKNTIHRVDLADNGQIGVAKFQNGHY
                     DLILMDMQMPVMDGITATRTIRRWEQEQGLPAVQIIALTALALKEESARIFEAGCNAH
                     MTKPLKRTTLLELLLAYEKTRAQ"
     gene            72845..73174
                     /locus_tag="PK224_04950"
     CDS             72845..73174
                     /locus_tag="PK224_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRB15113.1"
                     /translation="MMHHYAKPGPDKIPVRIDSSLEAIIPGFLANRQRDLTTIESCLK
                     QGDLNTIRMLGHRMKGDGGGYGFDQISVIGDQLERAAMAHNLSALHDQLNGLKDFLAR
                     VEIVYVH"
     gene            complement(73513..73588)
                     /locus_tag="PK224_04955"
     tRNA            complement(73513..73588)
                     /locus_tag="PK224_04955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73553..73555),aa:Ala,seq:cgc)
     gene            complement(73661..74335)
                     /gene="purN"
                     /locus_tag="PK224_04960"
     CDS             complement(73661..74335)
                     /gene="purN"
                     /locus_tag="PK224_04960"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRB15114.1"
                     /translation="MSGKSPRLVRLGVLVSGRGSNLQAIIDAIEAGTLSAEVAVVLSN
                     KQDAGGLERARKHGAPAVWLDPKPYAGRPDSREAFDKAVLEVLQKHEVDLVLLAGYMK
                     IVTAVLVSAYENRMMNIHPSLLPSFPGLDVQKKAIDHGCKIAGCTVHFVTEGVDEGPI
                     IIQAAVPIREGDTPDTLAARILEQEHRIYPRAIQLYAEGRLRVEGRRVSVADAGPVAT
                     GFHNPG"
     gene            complement(74325..75362)
                     /gene="purM"
                     /locus_tag="PK224_04965"
     CDS             complement(74325..75362)
                     /gene="purM"
                     /locus_tag="PK224_04965"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRB15115.1"
                     /translation="MTTYRDAGVDIDAGDEFVERIKPHVRATFRPEVMTDLGGFGGLF
                     RFQANRYQDPVLVSGTDGVGTKLKIAFLMDKHDTVGIDLVAMCVNDIAVSGAEPLFFL
                     DYFATGKLSLKTAEAVVRGISDGCRQAGCALIGGETAEMPSFYAEGEYDLAGFAVGVV
                     DRPKMIDGRQIVPGDAVIGLASTGVHSNGFSLVRKVLFEKSRLTVESVTPELGGALGE
                     TLLTPTRIYAKQVLSLAEACPIKGIAHITGGGITENLPRVFPARCGARIRRGSWPVLP
                     IFQTIQDRGGVELSEMYRVFNMGIGLILVVAPEHVDRVMAKAVEAGDRAYHIGDMVAQ
                     TTDEGVVEYVG"
     gene            complement(75609..77009)
                     /locus_tag="PK224_04970"
     CDS             complement(75609..77009)
                     /locus_tag="PK224_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB15116.1"
                     /translation="MSASILIVDDEVSILNSLSSILEDEGYEVSVAKSGIEALKLCAV
                     SPPELMMLDIWMPDMDGLETLRRLRELVPQTQVMMMSGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALDQHRLEQENRTLRTKVERKFELIGQSPAMRQLKQIIETAGPT
                     NSRVLIGGENGTGKELVARAIHQQSARANRPFVAVNCAAIPETLIESELFGHERGSFS
                     GATTMKRGQFEQADGGTLFLDEIADMSLSTQAKVLRALQEQQFTRVGGTKLIKVDVRV
                     LAASNKDLLKEIEKGTFREDLFYRLNVVPIVVPTLRDRREDIPLLVKHFLHVHAEEQG
                     LKIKQVSPEAMDVFIQYEWPGNIRELRNLIERLMIMVPGPVIEASHASVALQVRPQLS
                     ATQQAAGVQAPNSLLTRHYDSLRDARNAFEKEFIARKLREHHWNISRTAEDLQIERSH
                     LHRKIKLLDVEMRPEM"
     gene            complement(77042..79339)
                     /locus_tag="PK224_04975"
     CDS             complement(77042..79339)
                     /locus_tag="PK224_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15117.1"
                     /translation="MPESTDMTKLIPPGVLREKKPSTPASIESERRKRHVRPVWIVLV
                     LLLPCLALTFYYAQMAVPVGEESDSFLPSTGYAFVLLLVNLDLIGFVVLTLLLSRNLI
                     KAYFERRHRLVGSGFRAKLVAAFIGFSLIPTVLLALVASGLVNKAVDVWFNDQIEHVM
                     KDSYEVARMHHAGHVSLAINSARAISHEIFREELLLPEQRDLLVAAIARKRAEYATAG
                     IEVFSSKMETLTKSLDPEVPVAVLDLPIGQLVLQVINGKQELTSVQEAQTGRLVRAGV
                     PIASSIRRGEIDGVVVVDAYVPESLLGKMESIGRQYTEYKQMKAMKNPIKAGAYLLVA
                     VITVMILFSATWFGFYVARGITVPIQRLAEATEAIAQGDLSVRIEAKATDEIGTLVES
                     FNRMTGDLQSSKTKVEEANVSLRQSNLELDRRRAYIETVVDTIAAGLLSIDRQGIITT
                     FNPSAERMLGLWADRFRGRSANEVFKEYKLDLFQSVYDRMLVDQRDNIALEGQLDLQG
                     RFLTIGVHCSRMKDESNKDLGFVLIFEDLSELIKAQKAAAWREVAQRIAHEIKNPLTP
                     IQLSAQRLRKKFQDKAPDFDRICDESTQVIINEVGSLKQMLDEFSKFARMPAPHMTMN
                     SLDDVVKEVVALYDSAHREVECVVTLDADLPPFNFDREQIKRVLVNLCDNGIHAMNHK
                     GHLWITTRYDTKQRRAVVTVADEGTGIAPEDQEKLFVPYFSRKKTGTGLGLAIVRRIV
                     TDHDGQIHAGNHQPKGALFTFELPV"
     gene            complement(79544..79972)
                     /locus_tag="PK224_04980"
     CDS             complement(79544..79972)
                     /locus_tag="PK224_04980"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HRB15118.1"
                     /translation="MGDDKDQGFVIRDRRGRGEDAAPPAQAATPPAAEPSAAAPHHDH
                     DADSHTHSGHLPVNFSSFVISMGSSALMLMGEQLDPQQPSMPLNLPQAKEIIDLLSML
                     EEKTKGNLTPDEQVVMKDMLYALRMKFVSLTSGKSTLHTP"
     gene            80105..81010
                     /gene="mazG"
                     /locus_tag="PK224_04985"
     CDS             80105..81010
                     /gene="mazG"
                     /locus_tag="PK224_04985"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRB15119.1"
                     /translation="MSARFDNVVAIMARLRAPGGCPWDRKQTHESLKPYLIEETYEAL
                     DTIDRQDFAKLKEELGDVLLQVLFHSQIGTEAGTFTIDDVLEQLSDKLVRRHPHVFGD
                     GATSQSSLNSDQVVHRWEDIKRAERKNAGKPDSVLHDIPQALPALLRAYQTQVRAARV
                     GFDWPDSPQGLAAVLAKVEEEIGELKHALTDATARRSVAEPKPEPTPEMAAEMGDLLF
                     SLVNVARHIKVNPEESLRLATNRFTARFQFIEREAARSGRSVNDLSTVEMDHYWEAAK
                     LVESTAPAGTVPPLVPSTEPTRTHP"
     gene            81007..81621
                     /locus_tag="PK224_04990"
     CDS             81007..81621
                     /locus_tag="PK224_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15120.1"
                     /translation="MSTEQDPYEEGKRAGLHPLIVLFGAILVMWLLINLAVPSGKQKQ
                     AAGPEISQVTTEDPDAAPVMYKVQSTLTEFNAVSIVVPAQATTSQVVGLLKQFKQARL
                     AQSLPSMLPATSPGHKLGDQAVADLYIFSDPKYATPEAVGTLARGAHAPGNLYPQAIP
                     FEEAMEHVLGHYRIDLNDTSNPDTGSLGFADDSGVHSRQYRRIF"
     gene            complement(82003..82434)
                     /locus_tag="PK224_04995"
     CDS             complement(82003..82434)
                     /locus_tag="PK224_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15121.1"
                     /translation="MLNDQLVISDVTGPFREPREPVLSYDYSIQRATWAATHAVRVKI
                     AQAEELDYVKEKLLGTVTGSPGQQLMLNKFLSRKIGDQKVRIAEAEGWLKERGDVLVA
                     PFTGSLAHYFPQLEAWVQAEQDTLRAEIKNLVGLVASPPAV"
     gene            complement(82477..83931)
                     /gene="hemG"
                     /locus_tag="PK224_05000"
     CDS             complement(82477..83931)
                     /gene="hemG"
                     /locus_tag="PK224_05000"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HRB15122.1"
                     /translation="MARTPRSVVIIGGGISGLSTAFALQEQAAAADMALTCIILDAAP
                     VWGGKILTHRVGQLVMEAGPDSFLSQKPWGMELCRRLGLADQLINTNPVEKKASVLRG
                     GQLHELPEGLVTFTPTQLGPFFRSGLLSWVDLARMGCDVLIPPRRSTDDESLASFFRR
                     RFGRHACERVMEPLMAGIYAGDAEQMSLRATFPRFYELEQAHGSVIRGMMAARRARAQ
                     KVSGGGPRHTMFVTLKNGLADLVAGLTAQIQQAGGVLKAGVQAEALRVRSHQAGRWMY
                     DVICTDGTALSAEALVLATPAYVSAELVRPLTPMAAGLMDMIPYASTATISLIYPAEA
                     VGNRLQGFGFVVPRIEGRDLIAATWTSLKWPHRAPPEDVSVRCYLGGVGREAILQRDD
                     EALVQCVREELASIVGLLATPHYVEVNRWNRAMPQYTLGHLDRLAQLEAALSRFGGLA
                     VTGAGYRGVGLPDCIRDGAETAAKLLRYLQAAPV"
     gene            complement(84142..85086)
                     /gene="hemH"
                     /locus_tag="PK224_05005"
     CDS             complement(84142..85086)
                     /gene="hemH"
                     /locus_tag="PK224_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRB15123.1"
                     /translation="MSVTPQPVAVLLMAMGGPDCLENVEPYLQDVRGGRPTSPELVEE
                     IRERYRVTGGKSPVLDIAREVARALEQRLNRSGDARYRCYVGLRHWHPFIKETYAELL
                     DALPERIIGLCMAPQYSSLSIGAYMKKVEEARAELASETPISFVTSWHRHPLLIAAIV
                     ENIRRTLERFPAEVRAQVPVLFTAHSLPERVVAMKDPYPEEVQGTVQAVCAQLGTQPA
                     RFAYQSQGRSGEKWLGPSVEDTLGELAQEGHHHVLVAPIGFICDHVETLFDIDIELKQ
                     LALTKGLQLERILMLNASAPLIDILMSVVEAHESSLVH"
     gene            complement(85089..>86025)
                     /gene="hemE"
                     /locus_tag="PK224_05010"
     CDS             complement(85089..>86025)
                     /gene="hemE"
                     /locus_tag="PK224_05010"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRB15124.1"
                     /translation="EYRRLREKHSILELCKTPELAAQVTLQPIDRFALDAAIIFADIL
                     LPLEPMGLSLEFAEGEGPIIHNPVRDRAAVDRLKVIDDTGLQYVMDAISLTRKMLAGR
                     VPLIGFAGAPFTLASYAIEGGSSRNYIHTKQMMYREPETWHRLMDKFARVITGYLRRQ
                     IKAGAQAVQLFDSWVGCLSAGDYAEYVMPHVQLIFEGLKHEGVPLIHFGTGTTAILKA
                     MRQAGGDVIGIDWRIPIDEAWAMVGYDRAVQGNLDPVTLFGPISEIERRVTDILRRAA
                     GRPGHIFNLGHGILPNTPVENVAATIDLVHKLSTR"
BASE COUNT        18548 a        27433 c        22342 g        17702 t
ORIGIN      
        1 ctgggaaaat caattgctct tttaagccaa gcgtatagtt gccacggcca tcaaatgcct
       61 tgggggatat gccacgaaag tcacggattc gtgggagcgc gagagaaatt aaacggtcga
      121 ggaactccca catgcgtcgg cttctgagcg taaccttcgc accgatgggc atgccttgcc
      181 tcaacttaaa accggcgatc gcctttttcg ctcttgtagt gacaggcttt tgaccggtga
      241 tgatcccgag ctcattcgac gcactctcaa gtaacttgac gttctgaatc gcctcaccca
      301 tacccacgtt caagacaatt ctttcaagcc ttggaacctg catcaaattt ccgtagctga
      361 actctttcat gagagccgga accacctgtt cccgataagt ctccctgagt cgcggcgcga
      421 actgatgctc attgccggag tcctgagaga tctcaggaac ggccgcttcc ttctttgctg
      481 acgactttgg agtagctcct ttgccgcttt tacccttctc tacttttgcc atgaacgtct
      541 ttcctgttcc gactattcga cgatttcctt ggatttctta ctcagccggg cccgccgacc
      601 atcttcctgg cgcttgaccc ccaaccgagt tggcttcttc gtaactggac acaaaaacat
      661 cacattggaa atggcaagcg gggcctcgcg ctcaagaatc ccaccttgac gaagtttttg
      721 attcggcttg gtgtgacgct tgatcatgtt caatttttca accaagacct tgccgtttat
      781 agtgtccaca gacaagacct tgcctgactt gccacgctca cgcccggtca ccaccacgac
      841 ggtgtcgccc ttgcgaattt tcgttttgat tcgtgccatc cctaccagaa cctttcttcc
      901 ttcaactaca agacttccgg cgcaagcgag ataattttca taaacttctt ccagcgaagc
      961 tcacgagcga ctgggccgaa aatacgggta ccaacaggct ccccttgcgc gttgatcaac
     1021 acacaggcat tgcggtcaaa cttgatgtaa gacccgtcgt cacggcgcac ttccttcgta
     1081 gtacggacga tgactgcgcg actcacatca cccttcttca caccggcctg aggaatcgct
     1141 tccttgacgg ccacgacaac aatgtcgcct aacgatccgt accgacgcct ggtcccacca
     1201 agcacatgaa aacacatgac ttgcttcgcg cctgaattat cggccacatc catgtatgtg
     1261 taattctgaa tcatgccctg ccctactcac tgccaaggat caaatcacct cagcggttca
     1321 agtgtgttcc gtagttactt ttcaggttgc cccttgacca tgacgcgtac cacacgccaa
     1381 tgcttatctt tgctgatggg acgtgtctca ctgagctgta cccgatcccc tactttgcac
     1441 acgttacctt catcgtgcgc cttaagctta gtcacccggc gaagaacctt cttgtagatg
     1501 ggatgaatta ccgagcgctc cacggcaacg accaccgtct tattcatctt gttgctgacg
     1561 acgttgccat accattcacg tcgatgttgt tggccttcct tcatacgacc cttctctcct
     1621 acttagcaac cggttggtcg gcttgtgtct gcagcaattc cagttggcgc ctgaccgttt
     1681 tgagccgagc aatatctcgc ttcgtactcc gcacttgcat cgggttctcc aaccgcccga
     1741 tcccaagctg gaatcggaaa ttaaacagtt catgacgcaa ctgcttttct ttttcggcca
     1801 actcatcaat tgagagaccg ctcagatctt tcacatccat cacatcacca ctcaacacgc
     1861 gctacaggtt actgaaactc accgcggaca acgaatttgg ttgcgatagg aagcttgtaa
     1921 gcagccaact tcagtgcctg cttagcgaca tcgggagcca cgccacccat ttcatacatg
     1981 atccgacctg gcttgactac ggccacccaa tactcagggt tccccttacc tttacccatg
     2041 cgagtctctg ctggcttctt ggtaattggt ttatcgggga aaatccttgt ccacacctga
     2101 ccaccacgct tgacgaaacg agtgatagca atacgcgctg cctcaatttg gcggctggtg
     2161 acccaaccgg gctccaatgc cttcaagcca aactcaccaa gcgtgatagc tcctccgcgg
     2221 taggccttcc cccgcatccg tcccttttgc atttttctga acttaacttt ctttggcgct
     2281 aacacaaacc caccttttcc ttatcgaggc ttctcagcca agccggccta gcgccgagtc
     2341 tggtttcagt tgaagggcag gcaacagttc acccttgtag atccaggtct tcacaccaat
     2401 ctgccccatc gtggtatgcg cctcggcgaa accatagtcc acctcggcac gcaaggtatg
     2461 aaggggtaca cgcccttctc gataccactc ggttcgagcg atttctgcac cgcccaaacg
     2521 tcccgctacc atgatcttga ttccttgggc ccccagacga agagcagact gcacactgcg
     2581 cttcatcgcg cgacgaaatg caacgcgctt ctccaattgg gtcgccacat tctcgctaac
     2641 caattgcgca tccaattcag gcttcttaat ttctttgacc gtgatgtacg cctgaccaga
     2701 gtattccttc tccagagcgg ccttgagctt gtcaacctcg gcgcctttgc gcccaatgat
     2761 aatgcctgga cgagccgtat ggatgatcac cctcgtttgg tcgcctgagc gctcaatttc
     2821 cactttggcg actccagcat gatacaactt ggctttcacc atctttcgga tcttaatgtc
     2881 ttgatgcaaa agcctggcat agtccttatc cgcgtaccag cgggagctcc acgtgtagtt
     2941 atacccgatg cgatacccaa ttggatgtgt cttctgaccc atgaagaata accctcagtc
     3001 taagtgtaaa agctctagct attcaccgcg gcgccaggag cagcaaccgc aattgtgata
     3061 tggctggtgc gcttctgaat tgagttggcc cggcccatcg atcgcgcacg aaaacgctta
     3121 taaattgggc cgcagttcac cacggcttga gacacccaca tttcgtcact atcccccaat
     3181 tccttttgct ccgcattggc gacggcggaa cgaaggagct tctcaatgac acgtgcggca
     3241 tgcttcggag tgtgccggag catagccaac gccatcggga cctgctgccc gcgaatcatg
     3301 tcgatgacaa tgcgcgcctt cctaggcgtc actctcacaa aacgtaatat tgcttttgct
     3361 tctgccatag ctgaccccac ccaaaccttc cgatccttca gcgaaccagc gattacttaa
     3421 gagccactgc tttttcggtc tttgcctgcc catgcccttt aaaaaaccgg gtaggagcaa
     3481 attcaccgag cttatggcca accatgtttt ctgtgacgaa gacgggaata aacttcttcc
     3541 cattgtgcac ggcaaacgtg tgcccaatca tatccggcac aaccgtagaa cgacgcgacc
     3601 aggttttaat cagcttccga tccttcgtct gattcatctg ctcaactttt ttcatcagat
     3661 gatcgtcaac gaatggaccc ttggtaattg aacgaggcat tctcggctct cctacttctt
     3721 cctacgcgag atgataaact tatcggtcgc cttgttcttg cgggtcttat agcccttagc
     3781 tggcgtaccc cagggagaca cagggtgagg attgccctgc cccgactttc cttctccacc
     3841 accatgcggg tggtcgacag ggttcataac cacacctcga acgtggggac gcttaccctt
     3901 ccaccgtgta cgaccagcct tgccgacaac aacgttttca tgatcaacgt taccgacctg
     3961 accaaccgta gccatgcaag tcgacaggac cttgcgcatc tcgccggact tcagacgaat
     4021 ctgaacgtag gccccatcac gccccataac ttgagcagat cccccggcgc tgcggatcaa
     4081 ctgaccaccc ttgccaggct tcaattcaat gttgtgaatc gtggtaccaa ggggaatatt
     4141 cacgagcggc aacgcattcc ccggccggac ctcggcgccg acaccggact gcacctcatc
     4201 attgacggac aatcctactg gggcaagaat gtaacgcttc tcaccatccg catagtgcag
     4261 caaggcaatt cttgatgatc gattgggatc gtattcaata gacgcgacct tagctggaat
     4321 accagcctta tcccgccgaa aatcgatctg acgataaagt cgcttatgcc cacccccacg
     4381 aaatcgaatg gtttggcgtc cgtcattgtt acgcccgccc gtccggagat ggaagccggt
     4441 caatgacttc tcaggcttct tcttagtcag cttctctccc tggatagcgg tcatgccacg
     4501 ccgaccaggt gtcgtcggtt tataaacttt taatgccata gctgattctc actctatcga
     4561 gaggcccacc ggctaaacgc tttcgaatag ctccagcttc tcgccttctt tcaacgtgac
     4621 aaatgctttc ttccaatcag agcgttttcc aacaaatcga ccgagtcgct ttaccttgcc
     4681 gcgaatattg accacgttca cgcgggagac cttgaccttt aaaagcgatt caacggcctg
     4741 cctgatctga atcttattcg catcaggatg caccagaaac ccaacggtat taccttgctc
     4801 acgcaaggcc gtaatctttt cagtcaacaa cggctgtatt aatacttgat ggaggtcgcc
     4861 cttcatgacc acacctcctt aacccgattt acttcacttt gcgggatgac caaggagtga
     4921 catcggagga cgtcgtacac attcagctct tcaggcctta agaccgtgac attcgaaagg
     4981 ttctttcctg cctggacgag atgcgaattg gcgtcaccaa caacaagcaa tgccgtgccc
     5041 gaaatgccga gttgcgacag cgcgttggcg agcaacttgg tcttggcttg attgatggcc
     5101 aaatcagaca ctacaagaac attcccttcc agtacttttg ccgacaaggc gctctgcagc
     5161 gctgcacggt atttctttct tggcatgcca aacgcgtagc ttcttggctt cggaccgaaa
     5221 acggtccctc catgcctcca gacgggggag cgcaaagaac cagcacgagc ccgtccggta
     5281 tgcttttgct tccacggctt cttacccgaa ccactcacct cgcccctgcg caaagttgaa
     5341 gcggttccct ggcgatcaca agcacgctgc atcacaacgg cttcatgcac cagcgggaca
     5401 tgcggcttac agccgaatac ttcgctcggc aagtcaactg taccgacttt cttcttcttc
     5461 gagtcaacaa catcaacgat cggcatggct caactctttt tcgatttccg cacaataagc
     5521 aatccattca caccaccagg gactgctccg cggacgaaca acaggttttc atctggacgg
     5581 gcgtcgacca cctttagtcg ctgaaccgtg acgcgctcat cccccatatg cccaggcagg
     5641 gccttatttt tccaaacacg ggacggatac gaactggcgc cgatggatcc tggcgcgcgg
     5701 tggaacatag aaccgtgcgt ttcagggcca cccgcatagt tgtggcgccg aaccaccccc
     5761 tggaatccct tacctttaga gacaccgacc acatcaaccc aatcaccctt cttaaacata
     5821 tcgaccttaa tcgtctggcc gacagtcaca tccccgactt tcggaaactc gcgcaaccaa
     5881 cgactcggag gagcttgatg ctttttcaga tgaccgagct cgccctgcga caacctctgc
     5941 tccttgactt ccccgaaaga cagctgcaca gcctcgtagc cgtcacgctc tttcgtcttg
     6001 actgaaacga cgcggcaagg gcctgcctcg atcaccgtga ccggagccaa cacactctcg
     6061 tcataaactt gggtcatccc caactttttt cccaataatc cgtttgtcat cgtcttcctg
     6121 tcagctcaaa tgagattgac cgaacccaat cgtcagagct taatctccac gtcaacaccg
     6181 gccgccaaat tcaacttcat gagcgagtcc atcgtttccg gcgtcggctc cataatatct
     6241 agcagccgct tgtgagtgcg gatctcaaat tgctcacgag acttcttatc aacgtgagtc
     6301 gagcgctgca ctgtaaactt ttcaattcga gtcggcaagg gaatgggccc gactactcga
     6361 gcaccactac gccttacagt ctcaacgatt tcgactaccg attgatcaag cacgcgataa
     6421 tcaaaaccgc gcagcctgat tcgaattctt tgatcgactt tcacgctcaa ctcctcacat
     6481 gccggccaat ggaccgggac atcactacgc taaaatttcc gtgacgacgc ccgagccgac
     6541 ggtcttgccg ccctctcgaa ccgcaaaccg caatccctga tccatggcga tcgggctgat
     6601 caactcgccc gtgaccgtca cattgtcccc cggcatcacc atctccacgc ccggcgttaa
     6661 cgtcacgatc cccgtcacgt cggtcgtccg gaagtagaac tgcggccggt agccgttgaa
     6721 gaacggcgta tgacgtccgc cctcttcctt ggtcaacaca tagatctcgg ccttgaattt
     6781 cgtgtgcggc gtgatgctct tcggcttggc caacaccatg ccccgctcca catcctcttt
     6841 cttcgtgccg cgcaacaaga cgccgatgtt atcgcccgct tgcccctcat cgagcacctt
     6901 gcggaacatt tccacgcccg tcacgatggt cgtctgcgtg ggccgcaagc cgacgatttc
     6961 gatttcgtcg ccgaccttca cgatcccctt ctcgcaccgg cccgtcacca ccgtgccgcg
     7021 tccactgatc gtaaacacgt cctcgatcgg catcaggaac ggcttatcaa tggcccgggt
     7081 cggcgtcgga atgtaggtat cgacggcttc gagcaacttc agaatggacg gcacgcccaa
     7141 cggcccctga tccccctcga ccgccttcag cgccgacccc tgcacgatcg gaatcttatc
     7201 gcccgggaag tcgtacttcg tcagcagctc ccgcacttcg agctcgacca attccaacaa
     7261 ttccttgtcg tcgaccttgt ccgccttatt cagaaacacc acgatgtagg ggacgccgac
     7321 ttggcgcgcc aacaaaatgt gctcccgcgt ctgcggcatg ggaccgtcgg cggcactcac
     7381 caccaagatc gccccgtcca tctgcgcggc gccggtgatc atgttcttca cataatccgc
     7441 gtggcccggg cagtcaacgt gcgcatagtg ccggttgtcc gtctcgtact cgacgtggct
     7501 gatcgcgatc gtcatgatct tgctggcatc ccggcgcccc tgactctcgc tcgccttcgc
     7561 cacttcgtca tagctgatga atttcgccat cccgcgatcc gcgcagatct tggtcagcgc
     7621 actcgtcagc gtcgtcttgc catggtccac gtgcccgatc gtcccgatgt tcacgtgggg
     7681 ctttcgtcgc tcaaacttcg ccttcgccat ccccaatcct cccctaattc acaaaacgaa
     7741 acactttact cgccccgatg ctttgcggta attgcttccg caatttgacg cggcacctgg
     7801 tcgtagcggt cgaattccat gctgtatgtt gcgcgcccct gcgttctgga acgcaaatcg
     7861 gtcgcatagc cgaacatttc catgagcggc acggtcgcct cgatagcctg agcaccggcg
     7921 cgcactttca tgccctgaac cttccctctg cgaccgttca aattgccaat gacatcaccc
     7981 atgaaatctt gaggcacgag cacctcaacc ttcatgatcg gctcaagcaa gaccggatca
     8041 gccttcttac aggcgtcagc aaatcccatc gaggcggcga tcttaaacgc catttcattc
     8101 gagtcgacat cgtggtaaga gccatcaatg acggtgacct tgacatcgcg caacgggaac
     8161 ccagccacga cgccggtctc catgcgctcc ttgacacctt tttcaatggc gggaatatat
     8221 tccttcggaa tggacccacc aacagtctta ttaataaact ccagcccctt accggactcc
     8281 gaaggctcta cagtcataac cacgtgaccg tactggccac gaccaccagt ctgcttgatg
     8341 tactttgact ccgcctcagc ctttcgacgg atggtttccc gaaacgcgac ttcaggcttg
     8401 ccgacattgg cctcaacctt aaattcacgc atcagccgat caacaatgat ctcaagatgc
     8461 aactcaccca taccggcaat aatcgtctgg gcagtctctt catcggtccg cacccggaac
     8521 gaaggatctt cctgcgccaa cttctgcaag gcgaatccca tcttttcctg gtccggcttg
     8581 gtctttggct caatcgccat agcaataacc ggctcaggga acttcataat ttcgagcagc
     8641 acaggctgct tctcatcggc cagcgtatca ccggtagtag cattcttaag accaaccgca
     8701 gcggcaatat caccggcgta gacgatatcg atctcttcac gcttgttggc gtgcatcttc
     8761 aacagacgac caacacgatc cttcgttcct ttggtcacat tcaggaccgg cgtaccggtc
     8821 ttcaacgtgc ccgagtagac acggaaaaat gtgagctgac cagcgaacgg atccgtcatg
     8881 atcttgaagg ccaaagccga aaacggctcg gaatcagagg gacgcctttc gacttccttc
     8941 gaagtattgg gatcgacgcc aatcacagga ggaatatcta ccggagaggg aaggaaatca
     9001 acgacgccat ctagaagttg ctgaacgccc ttattcttaa atgcggatcc gcacaagaca
     9061 ggcaccacct tcatggcgat agtcccggca cgaacgaccc gccgcacctc ctcctcgctc
     9121 aagggctgac cgttgatata cttctccatc acttgatcgt cgaactcagc cactgcatcg
     9181 agcatcttct gacgatactc attggcctga tcgaccatat cagcaggaat ctcaccgatc
     9241 ttatacttgg cgcccagcgt ctcgtcgtcg taaaaatagc ccttcatcga aatgagatcg
     9301 acagacccgc gaaactcagc ttcccgccca atcggaatct gaatgggcac gggattggca
     9361 cccagccgat cgataatcga ctgaacactg gcataaaagt ccgcgccaat tcgatccatc
     9421 ttattcatga aagcgatacg cgggacttcg tacttatcag cttgacgcca gacagtttca
     9481 gactgaggct cgacaccctg aacggagtca aaagcggcga ccgcaccatc cagcacacgc
     9541 agggaccgct cgacctcgat cgtaaagtct acgtgaccag gcgtatcgat aatattgata
     9601 cggtgttcac gccagaaaca cgtcgtagca gcagccgtga tcgtaatacc acgctcacgc
     9661 tcctgctcca tccaatccat ggtggcggcg ccctcatgaa cctcgcccat cttatgcgtc
     9721 attcccgtat agtagagaat tcgctctgta gtcgtagtct tgccggcatc aatgtgcgcc
     9781 atgatgccga tatttcttgt tcgctctaac ggtgtctgcc tagccacaac tcccccagaa
     9841 aaaacgaacg tgctgccttc cgagccgcaa accttccacc gcaatgccga atcttatccg
     9901 gccacattgc agcccggctc atcagcagca cgacacaaca ctaccaacga taatgagcaa
     9961 aagccttatt ggcttcggcc atccgatgaa catcctcgcg cttcttcact gacgcaccgg
    10021 tattattgga ggcatccaac aattcggcgg caagccgatc ctgcatactt ttcccgccac
    10081 gcgaacggga gtattcggta atccatcgca gagccagcga aacacgacga gacgggcgta
    10141 tctcaacagg aacctgataa gaagcaccac caacccgacg cgacttcacc tcaactaccg
    10201 gcttcacgtt atcaatggca gacttgaaaa tcttcatcgg atcaccacca ttggttttct
    10261 cctgaatcaa atcaaacgcc ccataacaga tccgctcagc agtgctcttc tttccaccgc
    10321 ccatcaggac attcaaaaac ttcccgacaa gcttatcgcg atacttagag tccggctgcg
    10381 cctctcgatg accaaaaaat tgtccgcgtg gcatcgttat cctattaaat aaaatcggtt
    10441 ttcactgcgc aatcacgcaa tgacacaatc aattactact tgggccgctt cgccccatac
    10501 ttcgaacgcg cctgcttccg atcggcaaca ccgacggcat ccaaggagcc acgaacgatg
    10561 tgataacgca cacctggcaa gtcctttacg cgaccaccac gaaccagcac aatcgagtgc
    10621 tcctggaggt tgtgcccgac accaggaata taagtcgtaa cttccatccc gttcgtcaga
    10681 cggacgcgag ccactttccg aagcgcggag ttaggcttct tcggagtggt agtatagaca
    10741 cggagacaga cgccgcgctt ttgcgggcag cgcttgagcg ccggactttt cgtctttgac
    10801 tttacgagcg tccgaccttt tcgcaccagc tgattaatcg ttggcatgca ctatactctt
    10861 tcttgaaaaa cagacagttg ctcaaaaatg ctcgtgagcg aagccgtcgg attatagaga
    10921 tgtgtactac ttatgtcaag aacgttcttt ttcttttcat ccattatttt ctggatcagc
    10981 gccggcggaa ccggctgcat gctgacccac ggcaaccggc tgcggttgac caccggacaa
    11041 ctccccaggg gccttggcac tggccacgaa cgtttcccga tactcctcaa atccgcttcc
    11101 ggcaggtatg aggcggccca cgatcacgtt ttccttcaag ccgaggagat tatcctcgcg
    11161 gccattgatc gcggcctcgg tcagcacgcg agtcgtctct tggaacgacg ccgcagaaat
    11221 gaagctatcc gtggtcaggg ctgccttggt gatgcccagc aagaccggct taccgagagc
    11281 cggcttcccg tctttctcca agactcgctg attttctaca tcgaagagcc ccttgctcac
    11341 ctgactgcct ggcaagaacg aggtatcccc tgggtcctca atcctgacct tgcgcagcat
    11401 ctgccgcacg atgatctcaa tatgcttgtc gttgatcgac acaccttgca agcgatagac
    11461 atcttgcact tcatccacca ggtatttctg cagctccttt ggcccgagca cgtccaggat
    11521 gtcgtgggga ttggctgaac cgtccatcaa cggctcaccg gcccgaaccc aatcgccctc
    11581 atggacgttg acgtgcttac ccttgggaat gaaatactcc ttgacgtcgc ccattttatt
    11641 gtcgaccagc accttccgca tacccttcac aaagccgcca tacgacactt cgccgtcgat
    11701 ctcactgatc accgcctgct ccttaggctt gcgtgcctca aagagttcgg caacgcgcgg
    11761 gagacctccg gtgatgtcct tggtcttggt cgtttcacgc ggaatcttgg ccagaacgtc
    11821 gccgggatgc accatcgcac ccttctcgac gaaaatgtga gcgccgaccg gtaagagata
    11881 gcgtgcaacc ggcgctcctc cagacccgga cactttggcc gtctttccgc tatcatcctt
    11941 gatcgagacg cgcggtcgaa gcgtggcccc gctctgctcg atgatgacct tgcgtgacag
    12001 tccggtcact tcgtcgaatt cttccttcat ggtgacgcct tcgagaatgt cgccataggc
    12061 gacctttccg ccagtctcgg tcaggatggt caacgagtaa ggatcccatt cgaccaattt
    12121 ctgaccaacg tcaacccgat cgccatcctt caccttgatc ttggcgccat agacaaccgg
    12181 atatttttcg cgttctcgac cgctgtcgtc aacgatcgcg attttggcgt tgcggttcat
    12241 gacaacccat tcgccctctt tgttccgaac cgcaatgccc gcattgtgca catcggcatt
    12301 tttcttcgca tccaggctca tgtatttcaa atgacccgcg tgcttggcct ccaacaccgt
    12361 ctgttcgacc actttactgg ccgtaccacc gatatggaat gtacgcatcg tcaactgagt
    12421 accaggctca ccgatggatt gcgccgcaat gactccaaca ggctcgcctt tttcaacgag
    12481 acgtccgcgg gagagatcac gtccgtagca gagtcggcag acgccacgag cagattggca
    12541 ggtcagcact gatcggattt tgacccgatc gacgccggct tccaccaccg ccttcgcctg
    12601 ctcttcgtcg atttcttcgt tgaaggaaac gatgatctca cccgtcaccg gatcgcgaat
    12661 gtcctcgccg gcaagccggc caaggaggcg ctcttccaag ggctgaatga tttctccgcc
    12721 ttcgaggagg gcactcacca agataccatc cgtcgtgccg caatcctcct cggtcacgat
    12781 gacgtcctgg gcgatgtcga ccaatcgacg cgtcagataa cctgaattgg ccgtcttgag
    12841 cgccgtgtcc gccaaacctt ttcgggcacc gtgcgtggag ataaagtatt gcaacaccgt
    12901 caacccttca cggaagttcg ccgtgatcgg ggtttcgatg atttcaccgg atggtttggc
    12961 catcagtccg cgcataccgc cgagctgacg aatctgctgc gaactacccc tcgcaccaga
    13021 atcggccatc atgaagatcg gattgaagga ctcgatcttc gctggatcgc ctccggcgcc
    13081 caattccttc atcatctcat tggccacctg ctcggtgacg tgcgcccaaa tgtcgatgac
    13141 tttgttgtat cgctcaccgt tggtgatcaa accttcggaa tactgttttt cgatttcgtt
    13201 gacttcatgc tgggccttcc cgatcaggtc ttccttacgg gatggaatat gcatgttatc
    13261 gatacaaatg gacatccccg cccgggttgc gtagtggaac ccgagatcct tgatcttatc
    13321 caggaatgtc accgtctcgc gatgccccgc ctgccgatag accgcgtcga tcagcttcga
    13381 catttccttc ttagtcatca gcttattagc gtccgcgaac ggcatcatcg gaggcagaat
    13441 ttcggccagg atgacgcggc cggcggtcgt ctgcaccatc gttccgttga cccggacctt
    13501 aatccgcgca tgctcgtcca gcgctccggc atcgtacgca atccgcgctt cttcaggtga
    13561 gccgaacaac ttaccttcac ccttcgcgcc gacccgctcc ttggtcaacc agtaacaacc
    13621 aagcaccatg tcttgcgagg gaacggcaat cggtttcccg ttggccggag agagaatgtt
    13681 gttgatcgac atcatcagca cgcgagcttc gacctgcgcc tccacggaca acggcacgtg
    13741 gaccgccatt tggtctccgt cgaagtcggc gttgaatgcc gcgcaaacca acgggtgaag
    13801 ccggatcgcc ttgccctcaa ccaggaccgg atcaaaggcc tgaataccca atcggtgcag
    13861 ggttggagcg cgattgagta acaccgggtg ctcccggatg acttcatcca acacatccca
    13921 cacttcaggt cgctcttttt caaccagccg cttggcactc ttaatggtcg tggccgcgcc
    13981 acgctcttcc agcttgtgga agataaacgg cttgaacaat tccagggcca tttttttagg
    14041 caatccacac tggtgcagac gcaactccgg cccgacgacg ataacggttc ggcccgagta
    14101 gtccactcgc tttcctagca aattctgccg gaaacgccct tgcttgccct tgagcatgtc
    14161 gctcaacgac ttcaatggac gcttgtttgg cccgcgaatc gcccgaccgc gccggccgtt
    14221 atcgaacagc gcgtccactg cctcttgtaa catccgcatt tcattgcgga tgatgacacc
    14281 tggcgccttc aattcgatca aacgcttcaa gcgattattc cggttaatca ctcgacgata
    14341 gaggtcattg aggtctgacg tcgcaaaacg gcctccgtcc agcgggacga gtgggcgcaa
    14401 ttcaggcggc agcaccggga tgacatccat aatcatccac tccggcttat tgccggaacg
    14461 gcggaacgcc tccaaaacct tcaaacgctt agcatacttt ttcttgagcg cagcggaggc
    14521 agaggccttc gccttcacat gcaattcatc ccattgcgtg ttgatgtcga ctttcctgag
    14581 gagttcgcga atagcctcag cgccgatgcc gaccttgaac gcaccgctcc cgtactcaga
    14641 ctgcaaggag cgcagctgct cttccgagac caactcctgc tcgctcatgc tggtagagcc
    14701 tggatcgacc atgacgtagc tctcgaaata gagaatcttt tcgagctgct tgaggctcat
    14761 atcgagcaac gttccgattc ggctcggcac acccttcagg aaccagatat gcgcaaccgg
    14821 agcggcgagt tcgatgtgcc ccatccgttc acggcgcacc ttcgattgaa tgacttcgac
    14881 accgcacttg tcgcagacga tgccgcggtg tttcatccgc ttgtacttgc cgcaattgca
    14941 ctcccaatcc ttgatcggac cgaaaatctt ggcgcagaac aacccgtcct tctcaggctt
    15001 gaacgatcga taattgatcg tttctggctt cttgacttcg ccatatgacc acgaccggat
    15061 tttttccggc gacgcaatgc gaatccgcat cgagtcgaac gacaccgagt cgcgcggctt
    15121 ttcgaataat gtgtatacac cttccaaggt tgatccctcc ttaacggccg cctgagcgca
    15181 gttctgagtg gtgagctatc cggcttccga tctcaagact cagcactaga atcagtcctt
    15241 tgatttaacc agctcgacat cgagcccaag actttgcagc tctttgacta acacgttgaa
    15301 cgattccggc aatccaggct ccaggaacgg ttcgcccttc acaattgcct catacatgcg
    15361 ggaccggccc ggcacgtcgt cggatttcac agtcaggaat tcctggagaa tggacgcggc
    15421 cccgtaggcc tgaagtgccc acacctccat ctcccccaaa cgctgaccac cgaactgcgc
    15481 ctttcctccg agtggctgtt gcgtcacgag tgagtatggg ccgatcgacc gggcgtgaat
    15541 tttatcgtcc accaggtggt ggagcttcaa gacatacata tatccgaccg tgacggggct
    15601 gctgaaggat tcaccggtac gcccgtccat caacatcgtt tgtccgcttg gagacaactt
    15661 tgccttcttg agcagttctt taatctcttt ctcggaagcc ccgtcgaaca ccgggctcgc
    15721 caccttgatc cccaacgctc gggcggccca tcccaagtgc gtctccagga tttgcccaac
    15781 gttcatacgg gacggcacgc ccaacggatt cagaacgatt tcaactggag ttccgtccgg
    15841 caaatagggc atatcctctt ccggcagcac gcgtgagacg acgcccttgt tcccgtgtct
    15901 gcccgccatc ttgtcgccaa cctggatctt ccgcttcatc gcgatgtaga ccttgaccag
    15961 cttgatcaca ccaggcggaa gttcatcgcc tcgtctcagt cgaccgactt tttcgtcgta
    16021 cagggtctgc aggatttcaa tctgctcctt ggcacggcgc tcgacatcct ccagctcctt
    16081 ctgctcgtct ggatcgctca gaatgatgtg ccgcaccatg tcgtccggca accgcttcaa
    16141 aatttccgca gtgagcttgc ccttcttctt cagaatgaca tcgcccgtct caggatccat
    16201 cagatcccgt ccgaccacct taccaagtaa cagcttgcgg accttcttgg tcttttcatc
    16261 ttcaatgatc cgcaactctt cctggtggtc acgctgcaac ttcatgtgat cttcgctctc
    16321 gatactcttg gagcgttcat ccttatccaa gcctttgcgg gagaagatct tcacatcgac
    16381 gacgatgcct tccacgccgg gcggcaccgt gagggacgta tccttgacgt ccccggcctt
    16441 ctcaccgaaa attgcgcgca acagtttctc ttccggcgtc agttgagtct cacccttcgg
    16501 agtcaccttc ccgacgagga tgtcgccagg cttcacttcc gcgccgatcc ggataattcc
    16561 gctctcatcg agatcccgaa gcgcttcttc accgacattc ggaatgtcgc gggtaatatc
    16621 ttccttgcct aacttggtgt cgcgggcttc gacttcgaac tcctcaatat ggatggaggt
    16681 gaaggcatct tcccgcacca gcttttcgct caacagaatc gcgtcttcga agttataccc
    16741 gccccacggc atgaacgcga caagcacatt ctttccgagc gcgagctccc catgatcgat
    16801 agcgggacca tcggccagca cctgccccct cttgaccggc tcaccgatcc gaaccaccgg
    16861 ggtctgggtg atacaggtgt tctggttgga gcgttggaac ttgatcattt catacacatc
    16921 aaggccggaa tcgttgcgct tgcggccctc tttggcatca gcccgcacca caattctggt
    16981 agcatcgaca ctttccacga cgccttcgcg cttggcctgg acaacatagc ctgagtcacg
    17041 cgcaactacg gcttccatgc cggtgccgac cagcggcgat tcagccttca acagaggcac
    17101 cgcctggcgc tgcatgttcg atcccatcaa ggcgcggttc gcgtcgtcat gctcgaggaa
    17161 cggcaccaac gccgtagcaa cgctgacgac ctgcttcgga gacacgtcca tgtattcgat
    17221 cttgtcaggg gtcgcagtaa taaagtcccc ggctgaacgt gccgacaccg tctcggacac
    17281 cagtcgacct gaggtgtcaa ccttcgaatt ggcttgggcg ataatgtatt tgtctccctc
    17341 gatcgccgag agatactcca actcgtcact gacccgacct ttcacaacct tccgataggg
    17401 cgcctcgata aatccgaatt cattgatgcg cgcgtaggta gccagggacg taatcaagcc
    17461 gatgttcgga ccttccggcg tctcaatcgg acaaatgcgg ctgtagtgag aaggatggac
    17521 gtcgcgcact tcgaagcccg cgcgctcacg agtcaatccg ccaggcccaa gggccgagag
    17581 tcgacgcttg tgcgtaatct cggcgagggg attcgtttgg tccatgaact gggagagctg
    17641 actgctgctg aaaaactcct tcacggctgc cacgaccggc ttggcgttga tcaagtcgtg
    17701 cggcaacacg gtctccatat ccaggagatt catgcgctct ttgatgctcc gctccatacg
    17761 cacaaggccg agccggaact ggttttccaa caactcgccg acagaccgca cacggcggtt
    17821 ccccaagtgg tcgatgtcgt cgatctcacc gcgaccgatc ttgagattca ccagatagcg
    17881 caccacctcg acaatgtcct gcgccgtaag ggtccgctgt tcaagggcga aatcaaggcc
    17941 cagcttctta ttgagcttga gccgtccgac cggcgacaga tcgtaccgtt tcggacttaa
    18001 gaacaaatta tcgaacaggg ctcgcgcagt ttcgacagac ggggtctcac ccggcctgag
    18061 acggcgatag atctcgacca tcgcctcttc cttcgacccg gtgcgctcca tgtcgagcgt
    18121 atcgaggatg accagcgtgg cattggtcgt atccagataa atgaccttga attcctcgat
    18181 atcgctatca aggatcttct ccagaatctc ttcggtcaga cgctgatttt tctccgccag
    18241 cgactgcttc ttgccgctgt cgaccaactc cgtcagcacc gcgcgcccca ccaactcggc
    18301 gggggtgaca ggaatttcct tgatgcccgc cgccttcagc ttggcgatca gaaccttcgt
    18361 cagcttcgct ccttcgcgaa cgagcgggtc cttaccgcct ttctccatca cttccgtcga
    18421 acacttcaag ccgtgatgga tttcaggatc gagcttccgg tagagcttcc ccttggagac
    18481 acgaatctcc tccacggggt aatacatccg caacaggtcg tcggtgctat acccgaacgc
    18541 cttcaacagg atcgtggcgg gcattttccg acggcgatcg atgcgaacgt aaagaatgtc
    18601 ccgtgcgtcg aattcgaaat cgagccagga accgcgatag ggaataatcc gggcagagta
    18661 caataccttg ccgctggcat gggtccgccc cttatcgtgc gtgaacgacg cgccgggcga
    18721 gcggtgcaac tggctgacca ccactcgctc ggtcccgttg atgaggaacg taccacgctc
    18781 ggtcatgagc ggaagttcgc cgacatagac ttcctgctcc cgaacgtcga gcaccttctt
    18841 cttcggcccc ttgtcctctt tatcgaagac aatcaagcgc acacgcagct tcaaaggaac
    18901 ggcaaacgtc atgccctgct caagacattc ccgttcgtcg tacttcgggg tccccaacga
    18961 ataattggag aactccaagg ccgccgtgtt gttgtagtcg gtaatgggaa agacgctggc
    19021 taacgccgcc tgcaaaccct gatccttgcg acggtccggc tcgacttcca tctggaggaa
    19081 ctcttcgtag gaccgcttct ggatttcgat gagatcggga atatcgatgc tcgtgcgaat
    19141 ccgagaaaaa tccttacgtt cgacaaactc cgaaagagtc gattccgaca ttcgttagcc
    19201 ctccaagctc ataaggagat gaacgccctg cgccatccca acggacaact cacactgccg
    19261 cccaacaaac cgttacttaa cttcgacttt ggctccgctc tcctcgagct tcttcttcat
    19321 cgtgtcggct tcttccttcg tggcaccggc cttgatgggc ttcggagcgc cctcgaccag
    19381 gtccttcgct tccttgagtc cgaggctggt gagctcacgc accaccttga tgacctggat
    19441 cttcttgtct gccggagcac tgaccaggat cacgtcgaac gaggtcttct cttccgcagg
    19501 tgcggctgcc gctccgccac cacccaccgc cgcgaccgcc accggagctg ccgccgtgac
    19561 gccgaaacgc gtctcgaggc ccttcaccag atcggccaat tccaacacgc tcataccctc
    19621 gattgccttg atcaattcat cttgtgacaa cttggttcct gctgctgctg acatgtcgcc
    19681 ctcccctttc tttttatcct gaatggctgc aatgactcgc acaaacttgc ttaacactcc
    19741 gctcaacgcg tacaccacac cgcgaatcgg cccctgcatg gccgatagca gcatcgcaat
    19801 caacacttct tttttcggca gctgcgcaat ggcgttcaga tccgcagcct gcactagccg
    19861 gccttccagc actccgaccg tgaccttgat tttttcggag cgcttttcag cctgaatgaa
    19921 atcgcgcaag attttcgccg gaagaaccgg atcgtcatat ccgatcacca ggccggtcgg
    19981 tcccttgaaa tggtctttcg cgtccgcgag aatcgtgccg gtcgaggcgc gcacggccag
    20041 ggtattcttg atgacgcaat actccgcctt cgcgccgcgc aactgtctgc gcaactcggt
    20101 catctgattg acgggcatcc cggcgcactc cgtcagaatc gccaagcggg cgcgaccgaa
    20161 tttttccgtc aactctgcga tcgctgttgc cttctcttct ttcttcatgc ctctcccctt
    20221 actgagaacc tgcctgttct caactccaca acttcgacag cgccacagga tccagcttca
    20281 cgccgggccc catggtgctc gacatcgtca cactcttgat gtatcggccc ttgcaggagg
    20341 agggcttcgc ttttaccacc gactccagca cggcctgcgc attgtcatac agcttctgaa
    20401 cgtcaaacga gaccttaccg acaggcactt gcacgattcc ggctttctcg accttgaact
    20461 cgacacggcc cttgcgaatt tcactgacgg ccttgccgac ctcaaacgtg accgtgccgg
    20521 tcttcggatt gggcatcagc ccgcggggac cgagcacctt tccaagctta ccgacagagg
    20581 ccatcagatc aggcgtggag atcgcgcaat caaactccat ccacccgccc ttgatctttt
    20641 ccatcagatc atccgagccc acgtgatcag cgcccgcctg gcgagcctcc tgctcttttt
    20701 cgcccttggc aaatacgagc acacggagct tcttacccgt cccatgcggc aacgccgtgg
    20761 tcccgcgtac catctgatca gaccgcttgg gatcaatccc cagccgaatc gccagatcga
    20821 cggactcatc gtacttggca aaggccgcct gcttgacagc ctcgactgcc tctttcaatc
    20881 cataaaacct gggctcgatc ttttcttgag ccgcggtcaa tttctttccc atactgctgc
    20941 tccttcacat ccgccggagg ttcactctgc agaaacttac tggacggtga tacccatgct
    21001 acgcgcggtc ccctggataa tcttgatggc gccctcaaga tcggccgcat tcaggtcgga
    21061 caacttcttc tgcgcgattt cacggacctg ggcctgactc actttcccca ccttgtcctt
    21121 atgcggcacg cccgacccct taatgatccc ggcggccttc ttcagtaggt ccgatgccgg
    21181 aggcgtcttc atgatgaagg taaagctccg gtccttatag accgtaatga tcaccgggat
    21241 gatgctatcc ccgtccttct gcgttttggc gttaaactgc ttgcagaact ccataatgtt
    21301 cacgccgtgc tgacccaatg acggaccaac gggcggagca ggattggcct tgccggccgg
    21361 aatctgcaat ttaatctgcg ctgatacttc cttggccatg agttactccc tgagaactgc
    21421 ggactgagca cgagggctga gaacggctct cagtccacca tctcgaatcc agtgacctca
    21481 aatacgttcg acctgcaaga aaccaagctc gaccggcgta gaccggccaa aaatactcac
    21541 aaaaactttt acccggctat gatcggcatc cacgtcatcg accgcgccat taaagccgag
    21601 gaacggacca tccacaatgc gaacattgtc gcccttgata aaccggacct gctcgcgcgg
    21661 tcccgccgca ccggcatcca cctgcttcag caaggaatcg acctcttcgg aagacagcgg
    21721 agtgggctga gcccctccac cgacgaaccc agtcaccttg ggggtctcct tgatcatctg
    21781 caaggtttcg tcagcgagcg gcgcttccag ctcaatcaag acataccccg ggaaaaattt
    21841 tctccgggac gtacgtcgct ttccgtcctt gatctcgatc acatcttccg tgggaaccaa
    21901 gacctgccca agtcgctcgg tgagccccat ttgacttgcg cgctcaagga gactggtctt
    21961 cacgcgaccc tcaaaccccg cataggtatg gatgacgtac cagttcttat tgctcatcat
    22021 ctcctctcag ctgaacatcc agcatcgtat cgtccgggca acgccgaacc tgtccagccg
    22081 caccacgcaa ctaaatgacc ttgcgcatca gccagcccag cacggaatcc atcatcgaca
    22141 gataaaggga catgaggata cagaacacga tcacgaccgt ggtggcgccg accgtttccg
    22201 ccctcgtcgg gaaagagacc ttcttgagct caccgcgcac accgtcgatg aattctcgaa
    22261 tagaggcgat caatcgctga aacacggtta caatcctctc ttagaacttc acgggcactt
    22321 gattgaccag aaccagccgg caccggtcta cgcactcgtt cacttacaag ggcttggcag
    22381 gggcactagg atttgaacct agactctcgg ttttggagac cgatgtgctg ccgttgacac
    22441 catgcccctc cacccggaac atgagcgctg ccgagccaag ggccatgccc aacccagcag
    22501 aaaagcctac ttcacttcct tatgagcgat gtgcttccgg cagaacttac agaacttgtt
    22561 ccgctccaat cgatccggat cgttcttctt gttcttgcgg gtcgtataat ttcgctgctt
    22621 gcagaccgta caggccaaat cgatgatatc tcgcatttgc catcccctga gccgtcaact
    22681 ctcagccatc agccctaatt cacgagctga ccactgatcg ccgatcgcgc attcttacgc
    22741 taaaatttcc gtgacgacgc ccgagccgac ggtcttgccg ccctctcgaa ccgcaaaccg
    22801 caatccctga tccatggcga tcgggctgat caactcgccc gtgaccgtca cattgtcccc
    22861 cggcatcacc atctccacgc ccggcgttaa cgtcacgatc cccgtcacgt cggtcgtccg
    22921 gaagtagaac tgcggccggt agccgttgaa gaacggcgta tgacgtccgc cctcttcctt
    22981 ggtcaacaca tagatctcgg ccttgaattt cgtgtgcggc gtgatgctct tcggcttggc
    23041 caacaccatg ccccgctcca catcctcttt cttcgtgccg cgcaacaaga cgccgatgtt
    23101 atcgcccgct tgcccctcat cgagcacctt gcggaacatt tccacgcccg tcacgatggt
    23161 cgtctgcgtg ggccgcaagc cgacgatttc gatttcgtcg ccgaccttca cgatcccctt
    23221 ctcgcaccgg cccgtcacca ccgtgccgcg tccactgatc gtaaacacgt cctcgatcgg
    23281 catcaggaac ggcttatcaa tggcccgggt cggcgtcgga atgtaggtat cgacggcttc
    23341 gagcaacttc agaatggacg gcacgcccaa cggcccctga tccccctcga ccgccttcag
    23401 cgccgacccc tgcacgatcg gaatcttatc gcccgggaag tcgtacttcg tcagcagctc
    23461 ccgcacttcg agctcgacca attccaacaa ttccttgtcg tcgaccttgt ccgccttatt
    23521 cagaaacacc acgatgtagg ggacgccgac ttggcgcgcc aacaaaatgt gctcccgcgt
    23581 ctgcggcatg ggaccgtcgg cggcactcac caccaagatc gccccgtcca tctgcgcggc
    23641 gccggtgatc atgttcttca cataatccgc gtggcccggg cagtcaacgt gcgcatagtg
    23701 ccggttgtcc gtctcgtact cgacgtggct gatcgcgatc gtcatgatct tgctggcatc
    23761 ccggcgcccc tgactctcgc tcgccttcgc cacttcgtca tagctgatga atttcgccat
    23821 cccgcgatcc gcgcagatct tggtcagcgc actcgtcagc gtcgtcttgc catggtccac
    23881 gtgcccgatc gtcccgatgt tcacgtgggg ctttcgtcgc tcaaacttcg ccttcgccat
    23941 gattcactcc ttttctcaga acactgtttt cccaaaaccc taaccgtata ttccggctct
    24001 ccagcagcag acagcgaccc taaccggaca aacctgacca tggagcccac gacgcgaatc
    24061 gaacgcgtga cctcgtcctt accaaggacg tgctctgcca actgagctac gtgggccctg
    24121 aactcacagt cttagtccgc cctcacatgc tctgtccgca cccgcggtcg agcacgctgg
    24181 atttggagcg ggcgatggga tttgaaccca cgacagccag cttggaaggc tggaactcta
    24241 ccactgagct acgcccgccc acggtgaccc gaccaacact ccaacgatcc gacggcgaca
    24301 cgctcggcca cacaatgctg cgcaccgcca gatcgcgctc attatcattg cccaaccgga
    24361 cgcacacgtt tcacgtgaga ttcggtcgaa tacgctcagt ttggtgggca ggcaaggatt
    24421 cgaaccttgg aagccataag gcagcagatt tacagtctgc cccctttgtc cacttgggta
    24481 cctgcccgct aggcacacgt tcccaaactc aagcgaatat tatttatttc aaataaatcc
    24541 cgaaccagag ccacttcatt ccctgaagag aagaatgaaa gtaagcccca ccatgcacag
    24601 ctccccagca aaacaacagg gctggagcgc tgatgaaatg aggattgacc tggcttcgaa
    24661 agcagccgat tttatttgcg acgcgttcca ttgtcaaggg ggtatgttct atgcaggaac
    24721 aaatctacga tgcggcagga gcctcacagg ctccacggga ggcaggaaca accctcccgc
    24781 gcgtcaatac ctgatcagcc tggcgagtca tgttcgctgg actacccaaa acttccttca
    24841 tgagtaacaa gagttccgga gcagacaggc gaaagtccgg cgccagaaat cgcttgcgtg
    24901 ccatcgcctt ttcacttgga tcctccggct cgtaatatcc tctcaggctt gcgtcctgct
    24961 ccgcctgact gagccgggca atccaagccg gcacgttcag cgaccgaccg gatgatcgaa
    25021 gctcacgcgc tcccttcctc tccacttcca gttgggtcca gatcgcccat cccacaaaca
    25081 ccgcccaggt ttcattcaac gcttcccacg actccgcctc gctgagaaag cgctcctctt
    25141 tcaaatcttt cttttggagc accggcgtga tgaggacctg ctgatagcga caccgttgct
    25201 gctcgcgggc gaacgccata aacgacgcgt gggcggctga cggcgcacgc tcctgctcga
    25261 gaaaatccat gtaggcatga aacagttcgt gatagagcgt ttccaactcc ttgtgcgtca
    25321 accgcgccaa tggacgcaga gtcctccccg ccgcattcag ggagagcgat cgatccaaca
    25381 ccattcgatg ctcgaccggg tgatactccg cggcaaacgc gtgcagatcc tcaaactcaa
    25441 acgccacaaa ctgcgctgga atttgttcca ggaaccgagt aggaagatgg agggctttcg
    25501 cttccgccat caatgcgccc cagaggcgcg aagagtccga agatggcgcg gccgtctctg
    25561 cgctggcctg gccgaagatc agaagtccca gcaccagccc cgcggccatc gtgggcagca
    25621 cagctcggaa ccaaccgtta tagacgagca ccggacgcat gatgatcttg aatcgtagaa
    25681 tggagtacgg caacccaacc agagttatac ggtgggaacg atcgacctag acgtaccggt
    25741 catgctcgtg cccccactca tgcccatggc cttcttcgac cagagccaac gtttcaaaca
    25801 gacggggcga cacgtgatcc aggaatcgcg atggcttgga taacaacatg ccggaactcc
    25861 ggtcatacac attgatcgga taggtcaaga acaggtagcg cttcgcccga gtcaccgcga
    25921 catacaataa gcgccgctcc tcttccaact cttcatccgt attgaatgag aagaccgagg
    25981 ggaatttccc atccaccacc cataacaaaa atacgcactg ccactccagc cccttcgccg
    26041 aatgaatcgt cgacagcacc acctgctcgt cgtcgcgccc cgcaggttcc acacccaccg
    26101 cactgccatc cggcggggcc agcgctaaat cagcgaggaa ctctgccagg ctggggtaac
    26161 tctccgcgat ggtatgcaaa tggtccagat cccggatccg cttgggatag tcgtcatgat
    26221 gatctttcag gatcggcaga taatactcat agactcggtt gacctgctcg gtcgggctca
    26281 gatcatcact cttcgacagg ctgtcgagca ccagtgccag ctccttcaac tgcttgcccg
    26341 agcggccgct actgtcccgc agcacctgat acggatcgtt caccctcacc atggccgcca
    26401 ccagatcatg cgccttcttg ggaccgaccc cttcgaccag catgagcacg cgatgccaac
    26461 tgaccgcatc ctgaggattc acgaccaccc gcaaatgggc caggagatcc ttcacatgcg
    26521 ccgcctcaat gaacttgatc cctccccgct tcacgaacgg caagccgcag cgcgacagct
    26581 caatttccag atcgaaggag tgaaagctgg agcgaaagag cacggcaatc tcactgagcg
    26641 gcacgccttc ttcgcgcagt tccaggattt tttgcgccac aaagcgagac tgcgcattct
    26701 ccccggccgc ctccaccagc gccggcagcg gcccgtccaa cgtacgcgtg aacagatgtt
    26761 tcgtatattt ctcaggggct tcctgaatga tctcgttggc caggctgaga atcggctgcg
    26821 tgctgcggta attttcctcc agcttgtaga tcttggcgcc ggggaaccag gagggaaact
    26881 ccatgatgtt gcggaacgtc gcgccgcgga acgcgtagat ggactgtgaa tcgtcaccca
    26941 cgaccatgac attgtcgtga gtagccgcca gctttcgaat gagttctgcc tgcagccgat
    27001 tcgtgtcctg atactcatcc accaagatgt agcggaattg ttgcgagatg gtccgccggg
    27061 tgggttcgtc cttggtcaac aagtcccgca acagcaccag gagatcgtca taatcaagca
    27121 actggcgctg gcgcttcgca gcctgatagg cgcgctggag cttccctagc gcctccagat
    27181 ggtccgcaaa gtgggaaaac tcgtcgagga caatctcttc caatcctcgc aacgtgttct
    27241 cgcacttgct gtaaatctcc gcgatggtcc ccttgcgagg gaatcgctta tctttttcgt
    27301 tcaggcccag ttgagcacgc aagagggcaa tcagatcttc ggcatcgccg cgatccataa
    27361 tcgtaaagcc cggttcgatc ccaaggactc gtccatggcg ccgcagcagc atgttggcga
    27421 ccgaatggaa ggtgccgcca cagactcgct ggctacgcga tccgatcaaa gcgccgacac
    27481 gctccagcat ctcctccgaa gacttcctcg tgaaggtcag cagcaaaata tgcgagggat
    27541 ccaccccgga atcgatcaga taggcgacgc gatgcacgag cgtccgcgtt ttgccgcttc
    27601 cggcaccggc aatgaccagc gcagggccat cggccgccgt cacggccgca tactgctgcg
    27661 cgttcaactc cttggcataa tccaacgaga acttccggac gtggtcttga tccggagtcc
    27721 gtttcagaat gtagggctta ctctctcggt ccatgttggc atccgactgg gcagtgaggc
    27781 tcatgacggg agcggtcgcg ctgtataaca taccaccgga gaccttgcaa cgacacagtt
    27841 cgtgagtcgc ttgctccgat cgcgtccatc gagatcaaca cttggacctt caaattgagt
    27901 tgtatataat gccggaccag caggggactt ttatgacgac cagaccgcat tcgttcgctc
    27961 aacagatcca aaccgtggcg gagctcatgc tcgcggtcgc gggagagtcc gtctccgtga
    28021 tcaaacgggc ggctccggaa caggccttga agctcaaaca ccaggatgag tggaccattt
    28081 accttgaatt cctgcgcgtc atgttcaacc tgaccgaccg cgtgtcagcg ctccacatcc
    28141 cgctcaagga gcaaccgcaa ttcatggacg agttgaccga cacggtcatc gaccaactca
    28201 agaaaaccct ggaaccggcc ttcggtgccg gtagcgacca gacggaaatc gtactgacca
    28261 tcggaacggc cgtggccgaa agccgacaga cctatgagcg ctaccggttt ctcgtgacgg
    28321 aagacagtcc ggcaaagaac gacatgtacc gggatttcag tgatcgcgtc gcccgtgcga
    28381 tcagagttcc acgcagtccg ggtgtgaccg ccgcggccac gctctgtgtc agcgccgtca
    28441 ttccggccct catcgggatt ttcgaagggc aagcgccgcc cgtcgccgcc gatgcgacag
    28501 ccaacccggc cacggctggg ggaagcagcg cgcctcgggg agccaccggc gccgatatta
    28561 agctggtgag tgtcatgtcg agcatcaaag gcgaggaggt cgagacgcga tgggggctcc
    28621 atccgcgttt ccgccaggac ctgacgcaag aggaggcgaa acaactgacg acgaccatga
    28681 atcgagtcgc caaaattctc ggagagcgct acgccaccgt cgccttttct gaccaatggg
    28741 catcctggca caaagccgga cacgcctgac gcagcattca ggaaacggga caacacagtc
    28801 cccttcctca cccatcgatc acattccacg gaggcgtcgt gcaagcaccc gaccacatac
    28861 aaccccctca agatacggtt ccccaaagcc tgcatcgact cggtgaactg gcccgcaatc
    28921 tctggtggag ttggaaccaa ccggcacgtc agctgtttga gtccatcgac cctacactct
    28981 ggttcctcac ccatcacaac ccggtccagc tgctctccgg cgtgaaacct gaacgctttg
    29041 taaccttggc cggcgatccg aatttcgtcc ggcaatactc cgcggtgtta cgcgccttcg
    29101 acgaatatct gagtaataag aacacctggg cggcgacgga gttctccgca ctccagaatt
    29161 ctcccatcgc ctacttttcg gccgagttcg gactccacat ttctattcca atctatagcg
    29221 gcggactcgg cattcttgcc ggagaccatt gcaaagaagc cagcgacttg ggcattcctc
    29281 tcgtcggcat cggcttcatg tacccgcaag gatatttcaa gcagcgtatc aacccggaag
    29341 gatggcagga agccacctac gcccccttca atcgatacga ttcgccgatt caccaggcga
    29401 tgacgccgac ggggatcccc tgctccatca aggtcgagat cggccaccgc caggttacgg
    29461 tgctggtgtg gcaagtacgt aacggacgaa tgtccctcta tctcatcgat accgatgtgc
    29521 cggaaaacac gcccgaagat cgcgccctct ccgcgaggct ctacggtgga gaccaggaaa
    29581 tcaggctctg tcaggagttt ctgctcggga tcggcggcgt gcggatcttg cgcgccctgg
    29641 gcatcaatcc cgcggtctgg cattgcaatg aaggccattc ggccttcctg accgtagaac
    29701 gcatccggga attcgtcacc accggacaca gtcatgctga agcgagtgac ctggttcgac
    29761 agagcaccgt cttcaccacc cacacccccg ttccggccgg gcacgatatt tttccgtttc
    29821 acctgatgga tcgctacttc agcaattatt ggggccaact cggactctcc agagaagagt
    29881 tcctacgcct gggcgaaacg ccggaaaacg caggacacgg cttcaacatg accgcccttg
    29941 cgatgcggct gtcggcccac gtcaacggcg tgagccgcga gcacgggcga gtctctcgac
    30001 aaatgtggca acacttgtgg cctggcctgg cgcaggatct cgtccctatc cgcagcctca
    30061 ccaacggcat ccacgcgccc acctggatct ccccggagtc gaactccctc tatgccaaat
    30121 atctcagccc tgactgggcg gaacgggtcg atgaccccac catttggcaa cgggtcacgg
    30181 acctgccgga tgacgctctt tgggccgttc gacaaacaac gcgacgcaaa ctgatgcggt
    30241 ttattcgcga gcgcgcgcgg gacggctgga ttcgcggcca tctccagccc atgcaagcga
    30301 tcgccagggg aacgctgctg gacccggaag ccctcacgat cggatttgcc agacggttcg
    30361 cgacctacaa acgtgcgaca ctcctattcc gcgatctcga ccgactcaaa cagttgctcc
    30421 acaatcggtg gcggcccgtc cagatcgtgt ttgcaggcaa agcgcacccg gccgacgaac
    30481 ctgggcgcta cttcattcac gaggtgctga gtttctgcaa cgatcacaaa ctcggtggtc
    30541 acgtcgcttt cctcgaagac tatgacatgc atatggcaaa gtacctggtg caaggcgtcg
    30601 atatctggct gaacactccg agagccccgc tcgaggcgag cgggaccagc ggccaaaagg
    30661 ccgcgctcaa cggcgtgatt aacctcagtg tcctcgatgg ttggtggcag gaggggtaca
    30721 acggcgccaa cggatggggc attcaacccc tgcctgaggg atcggacacc caggcgcagg
    30781 acgctcacga tgcggagcaa ttgttccggt tgttggaaca ggaggtcgta ccgctgttct
    30841 accaacgcga cttggatggc attccacgcg gatggctcca gattgttaag gagagcatca
    30901 aaaccgtcgc cccccgcttc tgcaccaaac ggatggtcaa ggaatacatg aggcagctgt
    30961 atgcaccggc caccgcgcgc accccgaaca agtggtagca tgaccgggcc tgggccggag
    31021 ctcgctcgag tgcgccgaca cacgacctgg gtactgccag ttgggcttgc gctgcttgcc
    31081 tgggccatcc cgcattccgc aacggcagtc ccatcagcga ccaagcccgc cgtcaccccc
    31141 gtcgaacgac aagccatgac gctctaccag gcggctgaat acagccgcgt gctggagcta
    31201 ctccagcaga tacccgctgg ccagcaacca gcgttggaac tcctgcgtta tggcattctg
    31261 agtcatctca aacttggtaa gcctgaggaa gcgtggaagc tctacaccaa actcgttgcg
    31321 gacaatcgtc cagacgaacc agccgtattg cgggacattg cacgaagctt tattgtgact
    31381 cgtgtccgtg atactcagga acatatccgc attgctgcct atacggcgct ggcggaaatg
    31441 ggcgaaagcg acacgttacc gatactcgaa gacggcctgt tggattcatc ggcgctcgtc
    31501 cgcgctcgcg cagccgaagc cattggacga gccggcctgg ccggacgctc ctctgcgctc
    31561 aaacgagcgc tgcgcgacga ggcgcccgga gttcgcattg cggcaatcaa tgccctcagt
    31621 gatgccaaag tcgccggcat aacggatcaa ctgactgaaa tcgcccgcgt cgatgagggt
    31681 ccggaatcca tctttgcctt cgcaggattg tacaaacttg gacgaacgga cgtcctgacc
    31741 gacatctcca atgccgtcac gctccccgac cctgaggtgc gcatggcagc actaggtatg
    31801 ctcggacgat tgcgtcgacc tgccagcctg tcgatcttga gccaagccgt ctacgatccc
    31861 catcctgccg tccgtgcctt cgcagcgggg gctctaggag aattcggtag caccgacggg
    31921 atcgctccac tgacacacgc tctgagtgat gagaatccgc gcgtccgcag tgtcgccgct
    31981 tccagtctcg gtcgacttgg cgccactgaa actcgtagcg ttgtccaacc gctattgcga
    32041 gaccccgatg agcatgttcg tatcagcgcc gtcgaagctc tcctccgact cggtgatgcc
    32101 agcgcagttc tgaatgccgc agacctcgcc cgacatcccg atccctcagt acgtgggggt
    32161 gctgcgcatg ccctggcggc cgccaccacc cccaatgctc tgaccgtgct ggaaagctta
    32221 cttcgagacc agcagccgct cccacgactc atggccgcac gggcgctggg caaactgcaa
    32281 cagaaatccc tcatctctcc ccttaaaacc ggcctccaag attcggatcc cacggttcgg
    32341 atagcttcag caggaagcct tatccacata ttgtctcgaa aagacaaaca agggagcgcc
    32401 cgctaggggc tgagaatcag tatgctcaga tttatagggc tattgatgct actagccctc
    32461 tcgttcggat tcggatacct ctggggcaaa cagccggcga acaatcttga gcaaaaggtg
    32521 cgggatttct cgcggaatgt cgtggatacc accctgggag tagaacggga tctgcatcgc
    32581 cgccaaagcc tcgtcgacgc aaaatcaaaa gtggtgcaag cgaaggctga actttacaac
    32641 aaaaatacaa ccaatgctgc aaaggaactg gcagaggcta tcgactcact cgagctagcc
    32701 acccgtgggg ggaaacaagt agatcctaat gctcaggtaa aagagctggc aggaaaaatc
    32761 agagaacttc gcttggaact atccctcgga aagaaagttt ctgctacgaa gctcgacgac
    32821 gtccaaaaag aactagacct cctgctccgc aagtgaagcg gtcgttaaac agcggccggt
    32881 tgcttcttcc actttgcaag aatacgctca actcgcatct taaccaccat atcactgtct
    32941 ttcagcaatg gcttgatcgc ttcgcgacca cgctcatcgc cgatagcttc aagtgccgcg
    33001 gcagctgtca gccgagtgaa ccagtcctta ttctgcaaca tttcaatcag cggatcgatg
    33061 gcttcgggag acttaatccg ccccaacgca atcaccgctt gcttccgcac taactcttct
    33121 ttgtctttca acgccttgat caaaccaggc agagccggct caccaagatt aaccaaggca
    33181 tcggtggcat cctccatgaa ctcatcattt cggagctgtt gcatcagagg ctcaagcacc
    33241 cgttcatcac gaattttccc cagagccatg attgcggact tccggacatc ccaatctctg
    33301 agcaacttaa gcaaaacctc gacggcagga gaaccgatct gcccgatccc ctcaatcgca
    33361 acttcccgca cctgccagtc accatcgcga agagccgcag caagcggttc gacgcaacgc
    33421 tcatcgccca tttctcccag cgtgattgca gcttctcgcc tgaccaccca atcaggatcc
    33481 ttgagaagat cgatttgaat atcgatctcg tccttgacct gctcctcctc aagaaccact
    33541 tcctgcaatg gatcagtgag gtcctgggcg cctgccgaag aatccaagga agcagccagt
    33601 tgatccgaga cctgatctgt caaagtctca tcctcaacct gactaatggg ggaaacagcc
    33661 tgggcctgtc cttctttttc accagtgtgt tccatatcaa tgttccttgt tccgcagagg
    33721 ggtgggcgtg gcgatcaaat ccgtcaatcc cgcaaccgct taggacttcg cttcagtctg
    33781 atttccagca gcatgctttt ttctcaacgc aagcgcacgc cgtttgcgct gcactcgctt
    33841 gagccgtttg tgtaaagagc gggcagccgg atcacctgaa agttttgctt cttttgttac
    33901 tcgtaccgca atttttttct tgagtcggac ctcgtctgat tcaccaactg gtttctttgc
    33961 cattcagctt tcaacctcca acaattaaga tcaacaaaca taagaaatga cggcgcaatc
    34021 aggcatcact ccagaggagg cagtctaatc aagtacgggc ggaagtgtca actaacggag
    34081 taccgcccac ttaggatagg atgaggacgg ggacggtatt gtgagctgtg tggtcggctg
    34141 ccaacatact gccgatacgg cgctcacgcc tcacatagag cgcttcttgt ggcatcattc
    34201 ttacaggaac ggcaggtctt tcaactccct cctccgggcg acgatgttgc ctcactgctc
    34261 gcatatccgt attcaatacc ataccggaca cagcaggaat cttcgtcgag ggaatctgcg
    34321 cgaccggacg atgatctcca atcacctcaa tctgcaccgc cgtggtaccg gcttcaacca
    34381 tgcctaactc tcgagccgcc gcatgcgaaa gatccagcat acgccctgca acgtaaggcc
    34441 cccgatcgtt gattcgaact tgaacggttt tcccattggt caaattgacc acacgaacca
    34501 cactacctaa cggcagtttg cgatgagcag cagtgtaggc cgtcatatcg aaggcctccc
    34561 cattcgctgc gagttttcca tgaaactcct tgccgtacca tgaggccact ccccgatctt
    34621 taatgccgag gtccaatgta agctcgcctt tcggcaacgc ggaacaagca ctcaagagaa
    34681 gccagccagc cagaggaatc acagcgtaac cggccttacg acggaaaggg ttccgggagt
    34741 tcatgtccat agaagcacct cgtgtgaaag cctacacctc accgcctgtc agacaactct
    34801 cctactcagt gaggtcaggc tacgcaagat tgcccttggc gacaataccg tctgggtagc
    34861 agacgccccc tacaaatggg cagacaattc aacgctcaac gaagaagaag tctatgattt
    34921 tgctgaacag agccgaacaa cgacacaaat gaatttcttc cgctcactga tacgcaggat
    34981 gcctcaacgc ttggaactgc tgatagttcc agagtgaaca caagcccaaa ttgtcgggct
    35041 ctcggatcaa ccgtataaaa tcaggccagc ccgacagttc gatttcccac tggcaacacg
    35101 atctaacgaa atcgaatacc ccggtcttca aatttggtgt ttactacgaa ccgctcgaaa
    35161 ttcttctcca acacttctga caggatactt tccccatcct gcggctcgaa ccacaacaca
    35221 ttgtcatggc tacctgaatg agcgtcggtt cgtacgagtg agctgttatc ttctcggttg
    35281 ctcgcctgaa tgaccagttt ggttcgggct tcaatatcgg tgagaaatac cccgcgccgg
    35341 gcatcgaccg ccagttcgag aatcttcccg gaaagcacaa catcgggacc ggcttctgat
    35401 gctgccgggg cagtcttggc gtactgcgct tgccatcctt tgcgcttgag ataatcggca
    35461 aaagcctggg cggtttcctc gccagcacta ccgctcgaca cgttaaagta tgtgtcgcct
    35521 ccccacatcg aactccgact cccaaccttg gttcgatcgg gccgcccatc ttcaaacgga
    35581 atgaccatca ctcgaggccc cgccataggc ttcaccacct gaaccgcact agtggtatcc
    35641 gtttgcattt tgggggtcag gttcatcgga ataatgtcgc cttttccgga gcagcccaac
    35701 aggagcagca acccgacggc caccggcgca gatgtccccc gcaccacatg ctgtcgaatc
    35761 ataaaatctc tctacctcct tgtgagtaac aaatccataa ctggtgacta atcactgcct
    35821 aattgcgatg gagcacgatt gatcgaacgg cgaggccgtc acgagttttg acatacttca
    35881 gcgtcacttt gtctcccgca tgaatttgat cgagtccgat ccgctttttc ccgcgcacga
    35941 tggtggcacc ctgcttgagc accgcaccga ccaccgtggc ttcatttgct ccgtgctgcc
    36001 ccctgaccac gatgaccgga gggtcatctg ctaggttgac ggcttcaacc gttcctttga
    36061 tctcgtgcac cgtgttactg gcagcataga ccgaggtcag aaaacttccg aggccggcaa
    36121 gggcgacaca gagtgcggcg ccgatcacta acacaccgag caagctcggt ctcggctgat
    36181 tgatatcact cctcatccca gacgaacctc ttttcattca ttgtgcggag caacttgatg
    36241 gataaagtcg cggaacttta gcagttacac atcacgctgt aaagacggac gaggtctttt
    36301 cctgccgtcc cggaaagcca ggagccacat ccgtctcttg actgcaccga aggcggtcgg
    36361 taatatccag atcgatttgc cacaccacgt gccgtatcaa cgaacagcag gaatcacacc
    36421 catccagccg tctctctcga ctgcatgatc gaagttcgca acatcacaaa acggtatggc
    36481 aacctgacgg cgatcgaccg cgtcaccttt cgtgtggaca aaggtgaggt gctggcattc
    36541 ctcggcccga atggcgctgg aaaaaccacg accatgcgaa tcctgacctc tttcattccg
    36601 gcaacggaag ggacagcgca ggtcgcaggg ttcgattgtg cagagcaacc ggatgaggtg
    36661 aaaaagcgaa tcggctattt acccgagacg ccgccagtgt atcaagaact cactgtctcc
    36721 gaatacctcg ccttcgttgg acaactccgc ggactcagtg gcgcagagct gacggccggc
    36781 atggaccgtg tcatggaacg gctttcctta ggatctgtcc gccagcgact catcgccaat
    36841 ctgtcacgcg ggtaccgcca gcgagtcggc ctggcccaag cgctgattca cgatcccccg
    36901 gttctgatcc tggacgaacc cacggtaggt ctcgacccaa aacaaatcat cgaaattaga
    36961 gaattgatca agagcctggc cggctctcac tcagtgattt tgagcaccca cattctccct
    37021 gaagccacgg ccgtctgtca gcgtgtcgtg atcatcaacg gcggccggat cgtcgccgaa
    37081 gacacgcctg accaactgtc cgccaggctc cgacgatcgg aaaaaataag cgtgaccctc
    37141 aaaactccac cggcagatgt cctcgagcga tttcgcgagg tcgccggggt cgagcatgtg
    37201 ctgacgacgt ctgagcccca caccgttctc atcgagtgcg ccctcggcag agatatacga
    37261 gaagaagtcg cacggtttgc agtggggcag cattggggat tgcttgagat gaaaccggtc
    37321 tctatgacct tggaagacgt cttcctgaaa ctgactcagc gggaagacgg tctgccgaaa
    37381 gcggacgatg caagcgaaag tgaactacac tagcccgatg ccaccagttc aagccattat
    37441 cgcgaaagaa ctgcgatcct atttcgtctc cccgatcgtc tatgtcgtcg gcgccgtctt
    37501 cctgctcacc tttggcttcc tcgcttacct ctatattgtg tttaccggag ctcaagccat
    37561 tcagttgatg cagatgcagg gcggcaccgc gcagatcaat ctgaacgatc tggtcttccg
    37621 caatctcttt gccagcatgc gcttcgtgtt gctgttgatc ctccccattc tcaccatgcg
    37681 actcctggcc gaggaacgaa aactgcggac cttcgaattt ttgatgaccg cccccgtgcg
    37741 ggtcagtgag attattgtcg ggaaattcat cagcgtgtac cttgtgttcc tcggaatgct
    37801 gctcctgacc accctggttc cgctcacact ggccctcttc agcgatttcg attggtatcc
    37861 cgtcctgacc ggctacctgg gcatgacact cctcggaggg ttctttctgg ccgtcggcct
    37921 ctttgcctca tccatcacag agaatcaaat cgtggccgcc tttaccagct tcggcctgct
    37981 cttgatgtgt tggctgttgg ccggcctcgg ctcgctgtta ggtgacacgc cggccggcca
    38041 cgtggtctcc tatctctcgt tcatggaaca cttcgatcat ttggttcgtg gcttggtcga
    38101 cacaaaagat ttggtttatt acgtcagtgg taccgtttta atgctattcc tcgcgcaccg
    38161 agtggtggat tcgtcacgat ggaaataact cgtcgccccc ttcctatcgg cgccatcgga
    38221 accgggctgg ccattgcggg agtgatcacc tacaccctgg taccggacaa actgtggctc
    38281 gtgacgctac tggaagggct tgccctggtc ttgctcattt gggcctttgc catccacttc
    38341 gaacggctca agacctttac ctcccaacga tcgacccgca tgggcgccaa cagtgcgctc
    38401 atggtggcgc tgtttctcgg catcctcgcc atcgtcaact tcctcgcggc gcgacattcg
    38461 atccgctggg acttctccga gaatcagaac tatacgctcg cgccggaaac ctatcgcgtc
    38521 cttcgcaacc tcacgcggga tatcagcatc acagtgttca cgcgggagaa ggacccaggg
    38581 taccaggcgt acaaggaacg cttcgacagt tatcggcagg ccagttccaa gctgaccgtc
    38641 caattcatcg accccgagcg tcagccgaaa gtcgcacaaa actatggcat caccagaacc
    38701 gatatcgcga tctttgaaag cggcccgcaa tcggttcgca ttacgtcccc ggccgaggta
    38761 gaaattaccg gcgcgctcgt tcgggtgtcc aaagatacca agaagcgggt tgtgtttctg
    38821 gaagggcatg gcgagcggag cctggacgat accgatcgcg gcgggctgac gctgaccaaa
    38881 gaagtgctac aaaagcaggg atatgacatc ggcaccctca ccttgctgca ggaatccgca
    38941 gtgcctgaga aaaccgatgt cctgatcatt gccggcccac ggcgagcgct gaccaaagaa
    39001 gagaaggaac gcatccaggc ctacgtggtg aaggggggac acgtcctggt gatggtcgat
    39061 cccgataccc agtcgaatat ggacgacctg ctcaaaacct ggggactcga actagggccg
    39121 ggggtcctgg tcgatctaca ggaccggctg gcgcagggag acctgaccgc cctcttggtc
    39181 cgcacgttta ccgaccacga aatcacccat gaattgacgg cggccgtgct gtttcccctg
    39241 gctcgccatc tcacgttcca ggaggaatca ggcaaagagt gggactatgt tcctctcgcg
    39301 cgcacctcgc cgcgcagctg ggccgaaacc gatatgaaag gacgcgtcgt cagctacacc
    39361 gaaaaggaag acgtccaagg ccccctcgcg ctggctgcag ccttgacccc caagcaagcg
    39421 cccgaagaag gcaaaccgcg ccccgccgta gtcgtcgtcg gcaactccgc atttgccagc
    39481 aacgggttta tcaacttcgt cggcaacagc gatttctttc aacgcacggt gggatggctc
    39541 tcggaagaac gggatctcat ctccctcacc cccaaagacc ttgctgtgcg tccctttacc
    39601 cccaatccgc tccaggaacg cattctgctc tacgttcagg ttattctgct cccggcgatg
    39661 actatcctgt gcggcctcct agtgtggcgg aagcggcgtc gtttgtaaga ccatggcgcg
    39721 ctactggtcg acgctcatac tggcaggcgt gttggcaggg cttggactgt acctctacct
    39781 cgtcgaactc cctgaccaac gtaccgaagt ggcaaccgcc acccaagcca aacaaattct
    39841 gccgttcacc gaggcacaga tcacctccct gacggttcgc agccaatcca gcgaggtggt
    39901 attgactcac acacctggcc aaccctggac cattaccgca ccccttcaaa ccgacgccga
    39961 ccagcggcag gtgcaggcct tgatccgcgc gttggtcatg ggcaaggtct cgcgccttgt
    40021 cgaaacacat ccagcctcat tggctccgtt cggcctcgac cacccttcaa cagtcgtcac
    40081 cctgaccgca ggagacaagc aggaaacgct ctcgatcggc gatgccggcc ccctgtcctc
    40141 caccctctat gtcctgagaa cgtccgacga ggccgtgctc ttgacggatc tggcccctaa
    40201 agacttcctg aaccgaaccc tgctctcatt ccgccgcaag gaattgttgc aggtcaatca
    40261 gcaggagacg gagcgactgc gcctcaccta tcccacaaca gaaatcgtgc tctacgcgtt
    40321 ggacgaaaag ccgaaaaaga aatggaaaat ccgctatccc attgaagctg agggggaccg
    40381 caccgagatc ctagccctcc tcttccgatt ggaagatctt aaagcgctct cgatcgtcga
    40441 tcaggggcct gagcgggaaa agctcgcacc catgctgacc aaacccaaaa taaaagtcac
    40501 cttgagtgcg gccggggtgg accacgtggt ccgcctctat cagcccgacc cggcaagtgg
    40561 agaagcctat gcccaaacca ccccggacgg cccgatatac aaaatcagcc cctcggcgat
    40621 caaggacctg accaaagacc tctttaccct ccaggataag cgactgctcg gagtggatac
    40681 cggcgacatc gccatgctct cgatcaagac acgcgaagag cagtacgtgc tcattcatga
    40741 ccggaacgaa tggatcctcg aggatcagcc gacagagaag cttcgtcagg acgtcgcgga
    40801 cctgctcgtc agccgtatcg tcaatctgcc ggcggaagaa cgtgtcctga aacaagccgg
    40861 cccgctggcg ccctatggcc tcgtggctcc cgtggctgaa ttcgtcgcta ccgggaagga
    40921 cgggcgcgtc gccggacgcc tcgcaatggg caatcaagtc ggcggactcg tgtatgccat
    40981 cggccatcga attccaggga tattccaggt acgccccgac ctgctgaaac aaattccctc
    41041 ccgactcgca ctcctgggcc agggtccggg gtcacccccc taaacgacct tccggaggtg
    41101 tttccagata gagaaggttt atgctactgt gcgcccatcg ctcttgatca aggaggatcc
    41161 ttcaatgacg aactcgcgtt ctcacggtgc tgtagttctg tccttatctc tgttgttact
    41221 cagtgcctgt gctacggaag aaaccatgac cggcggcgga tcgacaccat cttcgagcgc
    41281 tgccatgaaa gcctccgcgc aggcctatga ctcactgcaa tcctgcctgg cacgcatcca
    41341 atccggcggc acggcgggca cgcgtcagct cgccgaagaa ggctgtcaga gggatgaggc
    41401 gcttcgtcag tcgatcgccg ggaatgccac tgcaaagagt ggaaacaggg cggcggccgg
    41461 tgctgcaggt gatagccttg aagcctgcat ggcacgcatt cccaaggacg gctcagtcgg
    41521 gcaacggatg ttggccgaag agagctgcca gcgcgaccaa gccaatcgac gctagcaccc
    41581 tctcgcttct cgagaccgac tcacctccga aggtggcagc cgccccttcg gaggcgcgtc
    41641 acttaaggta gacaatcctc ttcgctttca gtatcgtatc ctcacgcccc cgtagctcag
    41701 ttggatagag cagcggtttc ctaaaccgcg ggtcgcacgt tcaattcgtg tcgggggcac
    41761 cattccatcc cacttcaact tgcactgctc ggtcacccgc tcgcccacgc ggtcgcccat
    41821 tctttcgcga ttgctccaag tcatggagct atcaccctcc tgtcctacct gtacctcacg
    41881 cctggcgcgc agggacttcc ggcagggccc gctttcgccc cccatttcgt tatgaaccct
    41941 gctcgttgtt ttctatccga attgggagcg cttgcccgac cggcgtctac cttttggacg
    42001 gctaccctta ggccttccgg ctcagcgcaa aatgttctca ttgcacagtt ctctacatcg
    42061 attcttgcgt tgcatcagct tagcttatta ggaaggcgct tctgagcgct ataggaatca
    42121 acgtgttagg gccagagcag agcacggtat cgagattgca ctggatgctc cacataacaa
    42181 aggagtttcc accatgattg agttcccgtt gatcctgatc ggttatgcac ttctcccgct
    42241 gattgccatc caactcatga tcatggctga ccggccccag aagacaggcg agcaaaacgc
    42301 ctttccgcag gtcgagatcc tgtgagactg cggcggttct gtcagccatg agcagaaccg
    42361 ccgcttttcc aggcacgttc cgcatccctg ccgcctgcct cggcactcgt tccagccgct
    42421 ctagcccctc gaccggtgcg ccgaacgatc tttcgcaatt cgcgtaagta ggtgagggta
    42481 gaggacctcg ccctgcttcg agagcgaagt ccgctcgacg aatagagcag acgtaggtag
    42541 aattgttcat gtataatacc gtcgtccgaa cggtctcttg attacaatcc tgggctcccg
    42601 acgtgctcgc agcacatgct cttccgaaga gacccatgaa accggcgaat ctgttcatct
    42661 gcctatcgct cctcttcttg gcaacagcgg gttgccatgg atacgcgttc atcgaaaccc
    42721 cgacgacgga tggcttccac tccaaactcc cctccccata caccagggcg gtcgtctggg
    42781 gcggacgtcc ggacacggtt cagagtgcga gtacatggtt gctcaaaaaa ggcgttctcg
    42841 tcgtggacca gacgaaggtg ttgcaggcgg cggccgacca gaaggtaagc ctcaccggtt
    42901 accagtatct ggaaacggat gtgctgcgaa tggccaaact cgtcggcgct cggctggtgg
    42961 tcttcagcaa tgccgaggtg ggttcctggg aagcactgga ttggagtagc ggctttcctc
    43021 acagccagcg cgtctattca gccaccgttg ccctcagggc cgtggatgta aataccggcg
    43081 aaatcgaatg gagcggcaag gctcaatcga ccgaacgctt cagcaatatc gaggaagggg
    43141 tcagcctcct cacctgtcat gccctcgcaa cggcctgggg tctgcgcaat cctggcgctg
    43201 tcgcgccgga gagcatttgc ccgcctgggt ccggcctcat gagcaccgaa atgagcccgc
    43261 cgaaggccca cgccgccccc gcctcgtcgg gcagggagaa tttgactccc tccatgaatt
    43321 actagctatt gccccgctgc ctggctctac agtccagccc ccggacccga ctcacaatct
    43381 ctgtccgaca atccctggac gtgcataatc gctcttttgt cgtatttttt gcctcacgcg
    43441 atatactgca agggtgccgt tccgcaacgt ccttgctccg gccgcgcata tggctcacta
    43501 ccttctcctc acattattga gtcttgcggt ggcctccacg acacatgctg cggagaatcg
    43561 tccgttttgg accgagcaag cgctctttca tttcggggag gacgtgttct tcacagggcg
    43621 agcttcatgc gctcctaaca tagaagacgg gcgccaacgc gcatacgtcg ccgccgtcca
    43681 agaggtcaaa aatttcactc gtacgcagga agtcggcggc tttcctatcg atacgcaaat
    43741 gattttcgaa gacctgcatc cggctgactg cgcgcagggg cttatcacgg tgtggcgatt
    43801 gctgcgcgtg ccgcgcaccg cgctggaatc actctcgaga cacagcagcc ccgggaagca
    43861 gaaagacatt tctccgatac aggcgcagat cacggcgatc cggaatctca ctccacgggt
    43921 gggcatgctg cgtgacgaga tctggaatcg atacggtctg cctcgctcca tctgggcgcg
    43981 cccggagcag ggagaactga tctgggacta ctcccagttc ggcttgatca ttgtgttcaa
    44041 tcaggacgat gtgttgacac gctggcggtt gaacggaccc caccctagat ccagtgaaga
    44101 tgaaccgatt ctatcgcgcc aggacaatcc gacgggcaaa gatcttccag ccatcgacct
    44161 caccgttcga cttcagcaac tcgaagagca acaagacctc gaccggagac gccaggccca
    44221 acgctattgc accatccgtt tcgcggatgt ccctgaatcc ctgcgcccca agcatggcgg
    44281 ttgcgaacaa cgcgaatacg aacgattgaa ggagcaacac cctccattct gaggcctcgg
    44341 gatacagaca ccgaacagag gagtccagca tggcccccgg tcaaaaacag ccctcgacga
    44401 tcttaatagt cgacgacgac cccagcgccc ttcttgtcag taccaaaccc ttgcgggatg
    44461 ccggctacac ggtgcttcag gcaccgggca gtgccgaagc gctcagactc tatgcagaac
    44521 accccagccc gatccacctc gtgatcgccg atgtctttct gcctccaccg ggctttcaac
    44581 tttccgtcga caggaacccc tatccccgcg tgaacggtct cgacatggtt gagcggctcc
    44641 tgcaagacaa gcgcgagatc cgcgtcctgc tcatgtccag cagcccgagt tctgagttgc
    44701 agaatcgcgg cctcgtgcga gcgggattac cctttctcaa gaaacccttt acaagcgaaa
    44761 cgctgttgtc gctggtccga gaggtcttag ccggtcctcc cgccgctccc gatcagaaaa
    44821 atgcgacacg gcccggcaac ggcgatgtcg agtgggttga ctaaccgcct gccccgcccg
    44881 ttcccgcctc cggactcacg gatggaaaca gggatttctt gccagatatc acctgcttgg
    44941 atagactttt ctcaatagag ggacaggaca catggcacga gcgagagcta gcgaaccgca
    45001 acgcagccga tcgaaacaga acgccgtcga agaactcaat cgtggcatca ccacgttgcg
    45061 tgaactgagc tcacagatcg atgacctgag cagagatgga ttcccgtatc gtgacgcagt
    45121 ccgcgccaga accgaacttt ccctccggga atcgatccgg cgactattcg gtgaaaagtc
    45181 gccggaatac caggcccaca agaatcacaa gctccgaatc ggcaatcgca cggagtcggc
    45241 acagagcacc actctgctca aagaattgat cgtccagctg gagaatcaga aggcagagct
    45301 tttgggcctc acaccggcag aaaccgcgcc acctccaccg acagcccctg aacgccccac
    45361 actgacggca gttcctccga ctcaacccgc tattccagtc gtgccacccc aggctccgag
    45421 caccgccccc ctcgcaaccg tcagtatgat caacgctccg gcaaccgtct cagtagcgac
    45481 gacaagcatc agccaacccg ctcagcccat cgctccgatc gttatgacaa cacccgtctc
    45541 gtctacttcc gcctccccgc cggtcaccca ggggaccgcc tcacaaccga ttcgcgatac
    45601 atcgatacaa cgcctgcccc cgcaaccacc atcacccccg ctgcactccg ccacatctga
    45661 agcccccggg atcccggctg agaaaactgc gccggcatcc ctgcccgctt caccatcact
    45721 gcccgcagaa cctgttgtcg ctgcgccatc aacgaatagc gcaacgccct cggagcagac
    45781 agtgcgcggc gaggccgccc cgacaccact accgacaatg gcgccggcgc ggtccacgat
    45841 tccatcggga accattcccc ccattccacg aatgaactcc gtcccggcac ctgtgccgac
    45901 tgctcctatc gagccccctc cgacaccgca gcaggcctcc aagccaacgg atatcccggc
    45961 acaaaaaccg gctccagccc ccgtcgcggt gattccggca agccaagcca caaccgagat
    46021 agccgccgaa aaacaagcgc gcgatcctcg accgcccgtt ccccaagttc gaaccgagat
    46081 atcgctccca ccagccatgc ccgcgctcgc atcagttctc gagtccaaac cggaaccgcc
    46141 ggtcatatct cccgtcaaac cgccagagac accaacggta ctcacaacgc cgctggatac
    46201 gaataggaac aatcatcaat cggagggcgc aacgctcgat acactccgtc gaatgtgtgc
    46261 gcgcttccac ctggtcgctc gacaactgcg gctgcgaaaa gaataccggc ctacactgga
    46321 gatcacggac gagtacgatc tgcaggacct gttttacgca cttctccggt tacaattcga
    46381 tgaggtgggc acggaagaat gggccccgcc ttatgccgac ggtgctcgcc ggacgagcta
    46441 tctcctcgac tgggaaaaaa cggtggtggt ggtcaaacag actcgctcag ggttgacgag
    46501 cagagacatc gccgagcaga tcgccacaga caaagcccat tactccgggc gtccgaacgg
    46561 agccacgttg ttgtgcttca tctacgatcc tgacgggcga gtgggcaacc cccgagggtt
    46621 ggaggcagat ctttcgacag tcggcgacac gtacagagta gaaatcatcg tggctcccaa
    46681 gtaacaccgc gcgacgccgg ctatcctgcc cgagccgagg gcgttctgtt gacttcatcc
    46741 ggagcaatcc gctaggtttc gattcgttgg caggcgttga cgccccacca cctcacggga
    46801 gatttactcc atgccggacc gagcctacgt attgatcaac gtgcatcccg gccaaaccgc
    46861 tgatgtcgtc aaagcccttg gggatattaa ggaaatcaaa caaatcgacc cctgttgggg
    46921 aaagcccgac atcttcacca tcgttgaaat tccccaccag gacgcactga cccaactcgt
    46981 gctcgcgaaa attcacgcca tccccggtgt cgatcagaca gatacgcaca tggtctatcg
    47041 ccttcaggag ggcaagccaa agtgagaact cgtgtctctg gcattgcgac gttcgtgctc
    47101 gtctcggcgc tcgccgggtg tgcgggtggc ccacccgccc cggagcccga tgtgatggag
    47161 gtgatacttc cggttcctgc cgagcaggtg aaaacggcac tgtccgatgt cctgacccaa
    47221 gggggctata cggtcgacca agatgacacc ggcaacatca cgaccggaat gcggcaggag
    47281 atcagaagcc cttggaacgg actattgcgc tggagattcg gcgtagggaa gactcgggtc
    47341 gaggcgcggg tcgtcccgca ggacgacagc accacacgat tacgactgca agtgttccat
    47401 cgcgggaaag acgggatatt cgattcgtgg gaggatgcag aaacaccgct gccgcaaagt
    47461 gccgcgaacg aaattcgcct gctcaagaac gcgcttcgac tattgtaggg tcatcctact
    47521 tctcctcggc ctcatcgccg aaatccaatc caaatcgctc cgccattcga tataacgtcc
    47581 gccgatcaat ccccagaatc ttcgccgcct tgaccttgtt accccgcgtt tctttgagta
    47641 cacgcgccag atgccgcttc tcaacctcat ctaaggtcaa cagcgcgtct tgatccccct
    47701 cggcgggttg ccccttgagc agctcacccg aatccggctc agccctgatc gattcgggaa
    47761 gatcttcggg caacagcagc ggaccatgac tgagcgagac cgcccgttcc accgcattct
    47821 ccagttcgcg cacattcccc ggccagtgat agcgcttcaa gagcgccatc gtttccggca
    47881 aaaatcccct cacatgtaac gactgcttgg cgtatttttg cagaaagtga tgcgcgagca
    47941 tcgtgatatc ctcgcgccgc tcggccagcg acggcaggac aatccgcacc acattgagac
    48001 gatagtataa gtcgtcgcga aactttcctt ccttcacgag cgtggctaag tcccgattgg
    48061 tcgcggcaat gatacgcacg tcgactttca ccgatgccgt actgcccaca cgccgcacct
    48121 cctgctcctg catcacccgc aggagcttga cctgaagcgc ctggccgaga tcgccgatct
    48181 catcgagaaa cagcgtgccg ctatcggcgg cctcgaacag cccggctttg gctccgacgg
    48241 ctcccgtaaa cgcccccttc tcatagccga acatttccga ctccaggaga tgatcgggca
    48301 aggcgccgca attgactgga atgaacggtt tgtcgcgacg cggactgttc aaatggacag
    48361 cccgcgcaat cagttctttc ccggttccac tttctccttc gatgagcacc gtgctccgcc
    48421 cctcggaaac acgggccacc agcttgtaca cctccagcat cgccgggctg ctgccgacca
    48481 actgcgacca gggttcgcgt gcgcgcgcat cttcacgaaa acgggcgttt tcccgcacca
    48541 ggcggcaatg ctccagactg cgctggacga cgagacggat ctcgtccctc ttaaacggct
    48601 ttgccaagta gtcgtaggcc ccttgcttga tcgcttcgat cgcgccctcc agagatccga
    48661 acgcggtcag caatacgatc gaggtgtctg ggctgaactt cttgaactcc ttcaacacga
    48721 gaaatccgtc gccttgttcc atccggatat cggtcaggac gacatcggcc cgcgattgtt
    48781 tgccgcgctc caccgcagcc tggccgctgg agaaggcctc aacctcatag ccctccttca
    48841 ccagcgcttc cgccaacaag gttcgggcgg cctcgtcgtc atccactacg aataacttcg
    48901 cctgactcat gactctccct ctcctatacc gctaaaccgc agccgagtcg gacggcaaac
    48961 caggcagcac cacagtcacc gtcgtgccct tgccgatctc actctcgagt gtcaaccggc
    49021 cgccgtgagc agacacagcc tcccggctca gaaataagcc taacccggtg cccttaccca
    49081 ccgccttcgt cgtgaagaat ggttggaagg ctcgctcgac atcctcccga ggcattccac
    49141 agcctgaatc actcaccacc agtgtgacca ccgtactgag attggaccgg cctgtgcaag
    49201 acgcgctctc catctcctcc gagaaagcct gacgaatggt cacggcgacc gtgacctggc
    49261 cccccgcccc ggtggccgcg agtgcattgc tcaggagatt gaccaacact tggtggatct
    49321 tttcggcatc ggcccacacc accagagccg gcgccacttc tgtgtgaagc gtcacaccct
    49381 tggcatggta tcccggttcc attagcgcta cgaccggctc gatcaggcgt tccgccggat
    49441 accatgtcgg ctcaggcttg cgttgccggg tggacgataa gagatcttga ataatccgca
    49501 ccacgcgatt caactgctca tcgataatac cgattcgttt ctgcatcgca ggggtgacac
    49561 cgggctcctc cgccaacgct tccacatgcc aggcaatcga atgtaacggt gtgccgacct
    49621 catgcgccac cgacgccacc aactggccca cggcagccag ccgttcggat cgcgccaact
    49681 tgtctttaat ctcgaccagc tgcgtattcg ccagcaacag gtgttcggtt tgcgtcgcca
    49741 gcgcggcctt ggcggcttct tcgcgggctt tccgctcctc ccatgtcacg ccgatatagg
    49801 ccaccccggt caacacacag gccaccataa tgcgattaaa cacgacggcg gtgaaccgtt
    49861 cccgcttctt acccggctgg aagagtccca caaatgtcac caggatgcac agcgccagcg
    49921 tcacgatcat gagccgccgg tttctgacgg tggccaccag taagatcggg agcacgaaac
    49981 cgaatgcgcc gacgacattc agcgggaaca tccattccat caccatgacg atgaggaaga
    50041 ggattgcggc gatgaccagg accacgtgat tccggctctt catcacgatg tcgtgccgcg
    50101 caggaaggac agtttaggca gttgcggccg gagggcggag agtacacacg gctgcggcga
    50161 gtgcttgtac gcgttcatcc cgttggagct cctccagaga cagcgacagt ttgcgcgacc
    50221 agttcggata ggcgtccacg gtgcccggga gattcatttg tgtcacttca ccgatcacat
    50281 cgtcaaggtt ggccatgacg acccaggccg gcgacgtcgc aagatagaca tgaatcgcac
    50341 ggcacagagc ggacgtcatc accggaacag aatcaggctg atcgctcacc ccggagggca
    50401 acaaccccgc cccgtttaag acgcgaagga tatgcccccg gtcgcgcgtg cgctcctcga
    50461 atgcttgctg tacggcctgt tcgttcgcat acatgccgag cctggcccgc aatcggatat
    50521 cttctcccgt ccaataaccg gtcagcgtcg gaaggtcgtg tgtcgtgacg accgcgagcg
    50581 attgctcggg ataggccgac ggagacttgc aggacccatc ccagttccgt tcgaaataaa
    50641 agactcggta ggagagaatc ttataccgcg ccagttgttc ccgcacatag tccggcaccg
    50701 tccccagatc ctctccgatc accagggtct gcgctcgcag actttccagc gcgagaatcc
    50761 gcagcaggtc ttcggccgga tactgcacat aggctcccgc ctctgcgctc atcccgcgcg
    50821 gcacccagaa cagccggaac agcgtcatga catggtcgat gcggatcgcc ccgccttgcc
    50881 ggagcgtttt cctgagcaac tcaatgaacg agcgatagcc cgtggacttg aggcgcagcg
    50941 gatgaaacgg agcgaatccc caattttgcc cttgcggcgc aaacgcgtcc gggggcgcgc
    51001 cgcagtccgc accgagcgca agcacgtcct gcagcatcca cgcctccgcc ccgttgcgat
    51061 cgctccccag tgcaagatcg ggatacaagc cgaccgtcat gtgcacctga gctgcccggg
    51121 tgttagccgc tcgcaactga tccgccgcca cccactgcat atattggaaa aaccggaccc
    51181 gcttgcgatg ccgtctggaa aattcacgca gcgccggcga accgggagcc cggtactgtg
    51241 ccggccactc gggccaaagc ttgggcgact gttcgatcag acgccgttcc tcttctagca
    51301 cctgaaataa cgcaaaccgc tccagcgact ccccttcgtc ccggataaat cgatgcaaca
    51361 accaaccacg cgcggtggtc ggctccagcg acggttcgat ccccgtatag ttattcttca
    51421 gaaactgacg gtaggcgaga tccagcatca tgcgcttcgc cttgattacg gattcggaat
    51481 ccaccagatc ggcggcgcgt gcctgctcca gctccttttg aaactctgca ctgttccgca
    51541 gccgttgcgc atccggcgac gcgtgatact ccgggagccg atcaaggtcg atatagagct
    51601 cgttcaaaaa tagccggctg gtcggggaat aagggctgag atggtgggga cgcgaatttt
    51661 tcagcgcatg caatggattc agcccgatga tgccggcgcc cagctccttg cctgcccatt
    51721 cgaccaaccg ggacaaatcc gtaaaatcgc ccacgcccca attggtctgc gaccgtagcg
    51781 aatagagctg caccgccaag ccccacactc ttccaccccg ggccagctcc tccggcacaa
    51841 aacaatgcgg cggggccacg accacgcgga gcgtacccct ggtcaccacc ttgctgccca
    51901 tggtgccaac cgacaaatcg taatagccga gcaccagatt ctgaaccaac ggcagttcaa
    51961 cgcgcaccat gcgccgcccg gccagcgaac ggacctcgca tggattcagg ccggggctgg
    52021 tttcttcgcg atgaacgatg gtcccttttt catccgtgag actccaggct accgcgacgt
    52081 gttgctcctc cccttcctcc agcgcggggc ggaatgtcca ctgcgtgggg ccataccctt
    52141 gctggagaat gagaatcgga tcgcagggtt gttgccaggg cgcctcgtcc aaggcggtca
    52201 gttcctgtga cagtactgcg cccgacccga cttgaaaacc catggcggac agaatggctc
    52261 gacgcgtttc gtcactggtc acgtggagcg taccggcgat gtcgtgatat tcgggtgaaa
    52321 taccgacccg tgaggcgagg agacgaagca ggtcttgctc ggtgccatca tacatagtgt
    52381 cgtgcacgag tgtgagtgaa gacctccatc aagtcaaggt atcctatgct tttccgcaga
    52441 gttgcgacat gactcacgcc ggtcgagttc gcgactgagc atcgatccga cgcattaccc
    52501 ttcacgcgac gaggctcatg gctgattgat agcggttccg ttccgggcac gagtgccgcg
    52561 cggcgtcgca ttgctgcgcc ccctcacgcg tggtagcata accttagcgt cgagaggcga
    52621 cgattgtctt tgctcatcag gtggaaccgg gcacggaaag ggaggtggct cgtatgaccc
    52681 cgcagctgtc gcattcagga agcggtacct tgctcgcaat cgccgctcaa tgtctcgtgt
    52741 tttccgcaac ggcccaggcc ttgcccgaac actcgcgggt cgatgtcacc ggggattatc
    52801 ggtacgccta tcacgagccg gaaaccgcat cagaagcaag gcaacatgcc tgcaccgaag
    52861 cgctctatca ggcaatcagc acctcagcgg cggtacgaga gcagaccgcc tcgattgtcg
    52921 actcgaaatt attccgcaat ctggtgcata ccctcgcctc gaagcatgtg accgatcaac
    52981 agatcattca gcagagcgaa acgggacgca cggtgtactg taaggtgaag gccgtcttcc
    53041 acgcagatga ggtggaacgg gtgctgatcg cacagacaac gggcggaacc gaacccggct
    53101 tggatcaaaa tcgcgcgctg aagatcgtca gcgcgcgcga agaggctgat ggcacactcg
    53161 tcatcgtgta ccaggcgctc aagcgtctcg attggctggg aaccgcctac caaggcagct
    53221 tgcgcgaatc agcggatgtg atggtggact tctatgacga tcaaggcgtc ctggtacgga
    53281 gcagtcgtca tccggccagg aaaaccgcga ccggtgacga tgtcatgaat ccaggcgagg
    53341 tgggcactct gcgaatcgcg aaaccgctca atgcgaaaac ctaccgtgtc tgggtggtga
    53401 agtagtccac cgaggctccg gggacaggac cggatgtctt ccgctcctgt ccccgtcccg
    53461 atactcccaa cacatttgca tccgtgagtc ttaccgatca ctcgccaggg gcggcatgtt
    53521 aatgccgaca cgttccaatc ccatgatgac cgtcaaggcc gacggcgagt gctgcacgat
    53581 ttccttctgc acttcgatac ggcgcagatc gacgaattcc ggcgccgtca gtcccaacga
    53641 ttcccgatag gcacgatcgg cgatcccacg ctgcttttcg gctttttcac gcgcctcttc
    53701 cgccttttgg aactcgacca tcgtgatctt gcgctgctcc tgaacaatgg tttgagtcgt
    53761 ctgctcgacg acgcccttcg gcggcagaat gctaccgacg acgactcgat tgagccggat
    53821 cggcaggttt tgcttgtcga tgagtttggt ctgcacttca cgcgcaatgg catcctgcag
    53881 tttcgtcctg gtcgtcggat cggtcgtcaa ctgaaacagc gggtactttt gcacttcttc
    53941 ccgaacgaac gtgcggaagg cttccttcac gttgttttgg taccagttgg tgccgtagcg
    54001 accgacgatc tccggcgagc gcccttcgac cacattggcg atcataaacg catcgaagga
    54061 gaccggagca ttttcagccg aaataatgtc gaaatgttcg gaatactgta atggccggac
    54121 atccacttcg atcaccttcg tcgtcggcgc aacccagacc cggcccgtct tggagggagc
    54181 cggatcaacg cctccgtggc cgaagaagaa cggttgttcg accatcaccc cctcatggcc
    54241 ggcctcgatg gccacgcaac ccgacgtcag cagcaatacc cccacagccc ccagcatgga
    54301 ccaggctccg tgtctcatag gttcctcctg tgaaaaggtc acaccaccga ggcccgcctg
    54361 gcgacgtact gcgggcatca acggcctcat gatggcatgc aaatacctta cgaaccgcac
    54421 gcagagattg tcaaacgaag gggtgttgag ggcaagtggt cgacggtgaa cagacagccg
    54481 acggggggat acgaaccggc cgtgacctta ataaatcccg gcctcccgct gcaaccatcg
    54541 cacatatcga atgatatgct ccacgtctcc ggacgtcacg ccttcgattt tcggcatgtt
    54601 gccgaactcc cagtggtgag cgcggacacc gttttccgcg gcccgctgaa acgccatgtc
    54661 ggcgtgatga ttcggttcat aaatcttatg caccagcggc ggaccttgct tggtcccgac
    54721 accacgcaca ccatgacagg cggcacaatt ggcagtgaat ttcgcctcac cgacctgtac
    54781 ctctgccggt gtcgctcctc cggccggttt tgccgcgtct tggttggttg cattggattc
    54841 acaggccgcc agggctgcga ccatcaccgc aaacccgact cccatcaccc agaccttcat
    54901 ctgaatactc ctgactctga ttcgcttcat cgcgggggct cagaaggcgg ggttgtcgtc
    54961 cgaggtccct gcgattgctg tagttgatcc cgaagcattt gcgtcgtgcg attcagcgtc
    55021 aaccgccgat acagccgcgc cgcaatcggt gtgacgatca ccagggcgat gatcaaatac
    55081 atgaaaaatt cttcaggcgt cataggcgca gctctccaga ctcttgtgtg atcgcagggg
    55141 aaagtacaaa cctggcgcac ggcacaacgc aaccatcagc ttagtcgcct cgatggtcgt
    55201 tagagtatcg cataggggac cggatccaca actccggcca gccggaagcc ttcacgccga
    55261 atccggcaac tgtcgcaccg gccgcaggcc acgccatcag cgccggggtc atagcaacta
    55321 tgcgtcaatt ccaagggggc ccgcagctcc tgtccacgtt gcacgatttc ggcttttgac
    55381 agcatcagca gaggcgcaca cacctcgaca gcccgcccct caatgcccat cttagtgcca
    55441 agcctggcga cgacttcaaa tgcgcggata aaatccggtc ggcagtccgg ataaccggaa
    55501 tagtccagca cattggcccc gaagtaaatt ctctgtgcct caagcgtttc ggcgtacgcc
    55561 agagccaaag acaaaaaaat ggtgttgcga gccggcacgt aggtgacggg aaccccgccc
    55621 gcccgttcct cttgggtgcg gtccttgggc acgctcacat cgtccgtgag agcggatcct
    55681 ccgaacgccc gcagatccac gtccaccacc acatgagcgg cggcactcaa ggcctgggca
    55741 acggccctcg cccgctcgac ttcgacacga tggcgctggc cgtaggagat agtcagacaa
    55801 tagagccggt agccatcctg attggcaatg gcagccgtga cggtagagtc caacccacca
    55861 ctgagcagga tcaccgcctt cgaatcgacg tgagtcatag ggaacaggaa agagccgggc
    55921 agactaccgg ggtttggaaa ctcgaccgcc agtcacgggc tgaggcgcgt cacaagctga
    55981 agagggacgg tgaaatggat gcggctagct cctgtgagct agtcgcgatc ctcttccttc
    56041 tcgattttct cgagcaattc ctgtcgagac tggcattgga cacagagctt ggcgaacggc
    56101 accgcctcca accgcttttc gctgacctcg atgccgcatt ccgcgcaaat cccgtatgtg
    56161 ccctcgctga gtcgagtcaa cgcctcatcg atggcctggc gcttccggtt ccgcatttcc
    56221 atcaacgaaa tgccgagttc gcgatccaag tccatgaggg cctgatcccc cacgtccctg
    56281 gccgattcca agcggcgctg ctgatcttcg gtcaacgatt ggccgaggtt cccctcgatc
    56341 tcgcgcatga tttcctggcg tttgctcagg agcattcgct gcaggacttc acgccggcgc
    56401 tgacgctctt ctcgttcctt gggtgtttct ttcagagtaa gcgctgccac aacgcgcgcg
    56461 gcaacactgt cttcgtcttt ttcagtcata gcagtaaccg gagccggccg ctcttttttc
    56521 acaggcttct caggagcggg ggcttttgct ttcgtcgttg gtgttttttt cttcgcgtgg
    56581 actttcgtcg ccatagagtc tcatgccctt tcgtgatgag tgcaaggcga aaaagtttgg
    56641 caggtatatc acgctataga tcgtttgaca agtacctagc tccccaagat cttggggatt
    56701 tacggataga tgatcataga atgcacgtcg taacccgcca gccgatcacg tccgttgagg
    56761 cctttcagct cgatcaggaa gtccagccca gcaatctccc cgccgagttg ccgcaccaag
    56821 tcgatcgtgg ccgctgccgt accgcctgtg gccagcaggt catccacgat caacactcgc
    56881 tccccgggtg acacggcgtc ccgatgaatc gccaacacgc tggatccgta ctccaggttg
    56941 tattcgacct cataaatatc ggcgggcagc ttccccggct tgcgcaccgg aacgaacccg
    57001 gcaccgagac gggcagccaa cgtcccaccc atgatgaagc ctcgcgactc gatcccgatg
    57061 atctttgcga tacgctgtcc ctcgtaacga gcggcaagct catccgcgat ggagcggaag
    57121 gcctgcgcat tcttgagaag ggtcgtaatg tcgtagaaga ggataccggg cttaggaaag
    57181 tctggaactt ctcgaatgag ggctttgtaa ttcatacgtg caagcgaatc gggacgttct
    57241 cataagagat ccgcccgctt catggttttc cgttcaataa tgccgcggag cctggccagt
    57301 gcagccgtct caatctggcg aacccgctca cgggtcagcc ccatctcttg gccgatctct
    57361 tctaacgtcc gcgactcggc cccgtcaagt ccaaaccgcg ccaagatcac ggtgcgctcc
    57421 ttttcaggca gctctttcac ccaggccatg agttcagccc gccgcatcac tccttcggtc
    57481 gtctccgccg gcgtcaggca gagcggatct tcgatcacat cgcgcagaaa cgtatcctgc
    57541 cggtcgctga tcgggctgtc gagggagcaa gtagtgcgga ccagctgctt caagtcatcg
    57601 acttccgcaa cgctggtttt cagcttggcg gcgacttcgt cggcccttgg ttcccgtccc
    57661 agctcatgca ccagctgctc aaccttgccc agataccggt tcaaccgttc gacgacatgg
    57721 accggcaacc ggacgagctt tccctgattg atgatggccc gttcgataaa ctgacgaatc
    57781 caccaggagg cgtaggtgct gaagcggaac ccccgcttgt aattgaactt ctctaccgct
    57841 ttgatcaggc caagattgcc ctcttcgacg atatccgcaa agggaaaccc ccggttcatg
    57901 taccgcttgc cgatgctgat caccaggcgg agattcgcct cgatcatctc ctggcgcgcc
    57961 gcttcgtctc cctccatgac ccgcttgccc aatgcctgtt cctgtttaaa attgagcagg
    58021 atcgacttgc gtacctcgcg cagataactc ttaatcgtgt cgaggccctc ggcccgtccg
    58081 gattccgccg gacgattgtc cgctccggac tcttcggcct cggcgtgact ccccgactcc
    58141 gcatcctcgt cctcatcccc ccgcgccacg cgtgggcggc gagccgagac gggtgaattc
    58201 accgcgttgt cctgtcgcac tgctgcacgt cgagccataa ttccgttacc tactacttaa
    58261 tcataaagtc ctgagatcgg cccgtggcgc gactccactc cacctgcacc tgtgtcacgc
    58321 gcgacccgac ggggcccttc ttgagatccg acaggaactc ctcaaggacg ccccggtttc
    58381 cttcagcatc taccgccacc tgcccgctat cgagattctc gacgtgaccc gtcaatccac
    58441 gcgcaaccgc catccgacag gtaaaggccc ggtacccaac cccctgcaca cgcccgctga
    58501 caagcattct cgcgcgcact gattcgccgg tgtcggtcat cgccgcgcag ccctcccact
    58561 ggtcgaacac caagcgcaga gcgagtaccg cgcctggcat tggatactct cacagctccc
    58621 agagagcgtc acgcgatttg caagtttacg cggcatcacc agactcatac gcctacctta
    58681 tcggcaggta cctggcgaag catgagcgtg ggcgcaagag gccaatccga tacgtggcgg
    58741 gagcaggaga gtcacagtga agagcgacac cgagagacaa gcgctgacgt aggatgccca
    58801 cccttgccgg taaaggagaa ccagctccga atcaagcagg ctgtgtctgg atggtgtcgg
    58861 gaagacagat atcccgcaca tattcgaggt gcggggtcgc acgaagtgcg gtttggtcgg
    58921 ggcgagagga tttgaacctc cgacccctgc gtcccgaacg cagtgcgcta ccgggctgcg
    58981 ctacgccccg accgggcaga accgaaaacg ggaagccgga ttgtcttaca tcacgggggt
    59041 aaaacgcaag ccgtgcgcat cggccacacc ttggcaggta attctgccgt tcatcacgtt
    59101 cacacccttg gcaagaccgg ggtctgatcg aatcgctttc tcgactccct ccgatgcgag
    59161 ccggactata taagggagcg tcgtgttggt caaggctaga gttgaggtat ggggcacgat
    59221 gccgggcatg ttcgtgacgc agtaatgcaa gacgccatcc accacataga ccggatccga
    59281 gtgcgtggtc ggcctggtgg tttcacaaca gcccccctga tcgaccgcca catccacaat
    59341 cacggaaccc ggcttcattt tctttactag tgctctcgaa acgaccttcg gcgcgcgtgc
    59401 cccggggact aacaccgctc cgataaccag atcggcttcg acgaccgcgc gttcgatagc
    59461 cgactctgtc gccgcgcatg tcgcgattcg tccgccgtac agatcgtcga gaaaccggag
    59521 ccgatcaaga tccagattga taacggtcac ccgcgctccc atgcccacgg cgattctgac
    59581 ggccgacgtt cccaccacgc cggcgcccaa caccacgaca tgagcaggcg gtaccccggg
    59641 cacgccggcc agcaggagac cgcgccctcc ctgtaccgtc ccgagataat gggccccgat
    59701 ctgcacagcc agacgcccgg caatctcact catcggccgc aacatcggca agctgtgatc
    59761 tctggcctcg acggtctcgt aggcaatggc cgtgatatcg gccgcgacca acgccttggt
    59821 caaatccggc aacgacgcca gatgcaaata ggtaaacagc acctgcccgg gtcgaaagag
    59881 tgggcactcg gacagctgcg gctccttcac cttcacgatc aggtccgcct gcttaaacac
    59941 ctcggctttg gagccggcca cctgaacgcc ggcctgtcga tacgcgtcgt ccgaaaatcc
    60001 actcccctgc cccgccgacg gctctaccac aacccgatgc cccgcctgta gcaacacacg
    60061 agcgccgtca ggcgtcagac tcacacgata ctcgtagtcc ttaatttcct tcggaacccc
    60121 gatgatcata gccgcctccc tgcacgccac accgcttcgc cctgataccc cgtattatac
    60181 atggccgagc gtgactaccg tcaggaacct gcgcaccttg ctacgcgaaa gatcagtgcg
    60241 gtaagattcg cagactatcg accatttccg cttaagagga gcacctcacg catggacgcc
    60301 ggatcctgta atgcctgcag cgccgcgggc gcaccattga tgaagttttc gctgggaaaa
    60361 gatttcttcg ggcggaccta tgaccggctg tcgcccgctt cggatcaaag tccgaaatgg
    60421 tattgtgaac cctgctccat gatgaagaat ctgcaacgtg atttccgaga tatccgcgct
    60481 gaattcgaca aactcgccaa ggggcaggcc tccgccttgt cggaacctga agcaaaacaa
    60541 cgggcgcagt tgcgcctcaa tgaaattgcc gccatcgcag gcaccccggc cgccggctca
    60601 tcgctcctca gctcgacgga cgtgaagcag ttgatcgagc aatttcatgc ccgcgtctaa
    60661 tccctcgcat cgcaccctgg aacctcggac ttgctgacat gaccggctat cagcgacaca
    60721 tttttgtctg caccaacaag cgcgaaccgg atgacgcgcg cggcagctgc tccaagctcg
    60781 gctccgatgc actccacgcc tgcttcaagc aggaggcaaa acgactcaac ctgaaaggcg
    60841 tcgtccgcgc caacaaagcc ggctgtctcg attattgcgc acaggggccg accgtggtgg
    60901 tctatcccga aggaatctgg tatcgcgtga cgtccgaggt tgacgtgaag gaaatcatgg
    60961 agcggcacat cgttaaggga gaggtggtcg aacgactctt gatgcccgat cagtccccgc
    61021 tcccgcttct ttctccattg aaagcctgaa gcgatcaccc cctgctgact ctcgcgagct
    61081 ctctatgcca gccgacgata aatggaaggc caacatggaa aaggtggcct ttatgaaagc
    61141 cttccccggg ctgctccgtc actgggaagc actcgccggc aaaacggtcg aagcggtcgc
    61201 accgctcaag agcaaggccg gcgcggccgc gctgatctgc acagacggat cgtttgtggt
    61261 gcttccgccg ctgacgacgg aaccctacga gttgggtgaa gccctgcagg caggccgcag
    61321 ctacctcgaa ccaaaacacc ccgaagccta tatcggatac gatcagctgc tgaagaaaga
    61381 caaggatgca caacgcaccg cccgcctgga aaatattctc ggggcgattc gcaacaatat
    61441 ggaacagatt ccagagttga aagaccggct caaagacctg gtcaaggagt ggaagtgagc
    61501 agtctcccac agaaaaacat gttcgagatc ttctcgcaag gtctcttcga aggagtgaaa
    61561 cccatgctgg ttattcgtga tcacctggtc cgccatcccg accgctgcac ccatcaggcc
    61621 atttgcgtcc ccatctgtcc gaccagcgcc tggctctcca cgccgcccta caaattcgat
    61681 ccttcccgtt gcctggaaag ctgccgacta tgcctcgacg cctgcccgtc acaagcgatc
    61741 tatgcggtgt tcaaaaaggg cgagaagttg ctggagccgc agaagaagta gcgggagtcg
    61801 cggcccgcag accgcgcacg gtctaccaca cactggacgt aatgaaatcc gatcatgatg
    61861 gggctgaacc tcaacgggat aatcaattgc ttgtgccgac ggtccgcacg gcgcagccct
    61921 ctcttggaca cctagaatgt cgcatttcta accgccggca gcagcagaaa tacggccagc
    61981 agcaccaacg cgaggggcat cgtgtcctgg aaagaaagac tgcttccgct ctcaagggcg
    62041 tgcctgctga gcactcctgc ccacaccaga ttgtaggcgc aagccacccc caggccccac
    62101 ctccgtagac gccataaccc cacagacgca agcgcagcgc atccgaattg gaacgacacg
    62161 gttgcgacct caacgagcag gtgtcttagt cctatcacgc gaggattcaa cagcaggccg
    62221 agcagggcaa ggaccgccag aaccatggcc ggagcaaacc atgtggcaac cactccatac
    62281 atcaccccga gcagtcggat catccagggc tccgcaatac atttcacggc aacggctgac
    62341 ctccagaatg tcggcaggta gacgattccc cgatcagacg atctgcttcc gcaagcgccc
    62401 ccagtaggcc gtccctacgt atccgcaacc gcccgtgaag gtggtcaacg ccatgacgcg
    62461 atagacgacg gagcgcggcg ctttgacgtt gaccgacggg tcgaggagat ccggcgcagc
    62521 ggccacacgt ttcagcgttt cacccgccaa cagaatcggc cacatacatg ccaatcgctg
    62581 gcgaatttca aggcgtggca gggccatggt gtacatccag ccctgatcca agtgttcgat
    62641 ggccattttg atcagccgca tcaacaccgg cctgaacttc ggcaggttct ctcgattcag
    62701 caaatcaacc gacgtcaggc ccgcctcacg gagaagcgat tccggcacat aacagcggcc
    62761 acggtggaga tcccgggcaa gatccttcac gatattcgtc agctgcaacc ccttgccgaa
    62821 tcgcaccccg atggctgaca ttttcttcac atcccagcct gacatcgacg gacagtgcgc
    62881 gcagaccatt cgggtccaga attcaccgac acaaccggcg acgtagtacg tgtactgatc
    62941 caattcgtcc agggtctgaa acgccgtcag gtcctgagcc gaatcgccct gaaaatgcat
    63001 aagatccatc gtcatcccgt ccggcaacac ccccatcaac catcgaatcc gctcttggtc
    63061 ggctggatcg aattgttcat acagcgtcaa acagtcttcc agccgctgaa gcaacacccg
    63121 ttctgcggag tcggcttgat gaggaatcag tgcggcttgg atcgcgtgca cgtccctccg
    63181 cgctataggg ccggaactga actgcgttcg aaactggttg agatagcgga gtcgcagctc
    63241 gcggccgatc aggtcggtat cggcaatcgt gtcggcggca cgcgcgaaga gataggccag
    63301 ccccatctgg tctcgtaccg tcgccggcag cacgttgagg gtcagataaa acgaccggga
    63361 aacctgcttg aggataccgt ggagcagttc gtgtcttgcg gagtcgatag ccgtggagca
    63421 accttcttat ctaacttcca gcacaccttc catgcccttt tcccgatgac tcggaaagaa
    63481 caggagcttc ttgtcgcaat agaagggata aagaccgggc ttcctcacgg taaactgcac
    63541 cgtgaccgtc cggcccgagg acacatcccg ttcaatcgat agtcccgccg cttcgttctt
    63601 gaggagaaaa ttgtgcggag taatgctggt gatgctggtc aggagcaatt cgaccggctt
    63661 ccctgcctgg acgatcacat gactgggcgt ataggagtaa ctgtcgagga tgagggtggc
    63721 tcgctgaatg ccgtcgagtt gatcgaccgg cacctgaagc ggcggaacct gaggtgcttg
    63781 atcggcagca tgcacaagcg taggggcgat gaccgtgccg cacatcagca cacagacaaa
    63841 cccgagctgc ggaaaaccag agaccctgct catgatgatc ggttctagca gaaggccgcg
    63901 gagtcggtca accgagccgg cgacttgact gaccggaaac ctggtattat ctcttcacat
    63961 cgagacgcga gaaatccact tgcccagggg ccaataatca actcaggatc cggtataata
    64021 acatccaagg ttcgttcctc tccgtagaac aagggagcga ggagaacggg cctccaagga
    64081 ggagagatat gaagtcgttg aagggtatcg ggctgcttct catttcgaac attctcattt
    64141 atctcaccct gtcgattacg gtcagggtgc tggtcaacgt cgtcctgccg gccttcggca
    64201 tcgatgtccg aggagccttc agtcaggaac tgctggtctg gtcgctggtg atcggtttcg
    64261 gcggcgcgtt catcagcttg ctcttttcca aacaaatggc ccgcgccatg ctggattgcg
    64321 ttcaaattac ccagccccgc acaaacgcgg aacaggtgat ttacgggtcc gtgcgggaga
    64381 ttgccgaacg gctccatatc accatgcccg aagtctgggt atatgaatcg cctgacccca
    64441 atgcctttgc cacgggaccg agcaagaata acgccatggt cgcggtctcg accgggttgc
    64501 tggccaacct gcgggaagac gaagtcaagg ctgtgctcgc ccacgagatg gggcatgtct
    64561 tcaacggcga tatgttttcc acgacggtgc tcgccggctt gatgaacacc ttcgtccatt
    64621 acatcagcaa ctttgtgtat caaatggtcg cccagccaca gggcggtgat cgagaagagg
    64681 gccagagcgg cagcccgatc ctgggtttcg tggtctatat cttcttgcag gtggtcctgt
    64741 ccgtgctggc catgatggtc gtcagctggc attcccgcca ccgggaatat gccgccgatg
    64801 ccttctcggc gaaggtctat gggaaagagt cgatgatcaa ggccttgcag gccatcgatc
    64861 gctgggtgaa ccgggctcag ttcgaatatt caacgcaaga tgctttggcc acgatgaaga
    64921 tttccggcaa cacctccgga tttctacact tgctggcgac tcatcctcca atcgaggaac
    64981 gtgtcgcggc gctggaacgt ctctaaccaa ggagtcttca tatgccgatc tctcgaccga
    65041 cttgttcatg gaaggtactg gtcctcggcc tcgcagtggg tctcgggctt ggcgcccttg
    65101 aaatgggcct tggttcggtc gatgcggcgg aacaaggagc gacacgcatg gcagctaaag
    65161 cgtctacggt ctatgacttc acgttgaacg atatcgacgg gaaacctgtc tcgttgggcc
    65221 aatacaaggg aaaggtcatc atgctggtca acaccgccag cttctgcggc aacacgccgc
    65281 agtattcaga cctcgaaaaa atgtacgaga cttacaagga caagggcttt gagattctgg
    65341 ccttccctgc gaataacttc ggccagcaag aaccggggac gaacgaggag atcaagggat
    65401 tttgcctgac caagtacagt gtcggcttcc cgctcttcag caagatcagc gtgaagggga
    65461 gcgacaaaca tcccctctac cgctatctca ccgagcagag cccattcccc ggtgaagtgg
    65521 aatggaactt ccagaaatat ctcgtcgatc gctcaggcaa cgtcgtcgcg cgctaccacc
    65581 atcgaaccaa accggtggct gacgaagtgg tgaaagacgt ggagcggttt ctcgcgaaga
    65641 actaaccggt tacgccttct tcacaaaagt gacatgggtt cgatactctt ggatggccgc
    65701 actcagtgcg gccttcccaa gaggaatatt ggcctccaag gcatcttcca ccgtctgatc
    65761 gtcaatgatc acgtcatact tgtcttgcag attggttcga accgcggtcc cgtccttgaa
    65821 cgtccgcggc atatagattt cagtccacac ttccttctca accaggcaga catcacagaa
    65881 ccgctcgtac aacagcgcca gtttctccgc atccgtctct ttcatgcacc tactcccggg
    65941 taaaacaatc gcaccacgcc ctcaagaggt ggtgagtgaa ggtagaccaa gcgacggggg
    66001 atattcaacg ggtggtggaa tcggacggcg caggcgcggg cggctggtcg gtcacggtaa
    66061 aacgcatggt cccgacctga ttcacggcat gaaagggctg ctgccccaac gatgtaatgt
    66121 aaatcttgac cagatacttt ccttgcggcc attgttcgga cggtttcttc aactccagaa
    66181 aaccataccg ttcgtggccc ggcacctcga gactatcggt cacgattgca cgagcaccgg
    66241 tcttcccgtc tgcgcccacg ggaaacacct gggcggcaaa ctgcgccggg tcctcaagcg
    66301 gagccacctc aaagacgatg tagacggccg gagtatccgg agagaacagc gtggttcctg
    66361 gagcgatggg cttcaagcgg ggatcctggg tgtaccatcc ctttctccca aacgtgtccc
    66421 actccacctc gtaaccgcgt gccatcttga tccacgtgaa cagcgattgg ggaacgcctt
    66481 tctcttggac atccatcgaa ggggtttccg tcggctcaaa ctccgtagtg gcctgctcct
    66541 tcggaaccat caagtcccgt ccctccacga ccgcaacgga gaatcccact ccagccatca
    66601 cacctaccat gacggccagg cggcagatga cccaccttcc gttcattttc atggctggtt
    66661 cgccgcaggc ggctgatatc gggagatcca tagaattatc gtactcactg cagagaaggg
    66721 ggtcaaccgg cctgcaggga ttcattccaa accccggcca tgtcgactgg ctctgatata
    66781 atccgaaaag ccatcacgta tcatagtgct catcatactt ctgtttccat ccacagcctc
    66841 atgaaccaac ccgcccccca gcaacagaca tcgctccccc ttcaccccct tgagccttca
    66901 ggcgatctta cgcagtcgtc cgatcaaaat gccaaggctg agagccgccg gtacggtgtc
    66961 cgcgatgcgg cctttcaagc ggccgcgcag ggtggcgggg aaaactattt ctcggccttt
    67021 gccctgtttc tgcatgcctc tccattccac atcggcatct tgtccgcgct gccccaattg
    67081 gtcggtgtgg tggcacagct cctgtccgtg aaactgctcc aatatctccg catgcctgcg
    67141 caactcctga tcggcggcgg ctgggcccag gccctctgct ggctgccgat cctctcgctc
    67201 ccgctgctcc tgcccgagca tggtccctgg ctcctgatcg gctgcgccat gctgtacttt
    67261 gccttcggac atgccacggc cccggtgtgg aatagcctgt tggtcgatgt cgtggagacg
    67321 agcagccggg gcgcctactt tgcccagcga gcccgcgtga cggcgttgac gagttttgtg
    67381 accttgggca ttgccggaac ggtcctgacg ttgggacaga aatgggaact cagctggatc
    67441 gggttcctct tcatttttct gggtgcggcc gccgcgcgag tgggagcgac ccgctgccaa
    67501 accaggatct ccaaactggt gccggtccac catatcgacg ctccgcatgg attcaggcat
    67561 tttcttgctc agcgcgccac gccggatttc cggcacttcc tgctgttctc cggactcatg
    67621 catttctcgg tattgatctc cggcccgttc tttgtcgtct atctgttgcg agatctacac
    67681 tggtcttacc tgcaatatgc aggctggatg gccagcagta tctcggcgca attcttcacc
    67741 ctcgcgccat ggggacagat cggcgatcgg tacgggaaca agaccctgct catggtcaca
    67801 ggcttggcgg tcccgctcct accgatgggt tacctgttga gcgaacacta tctgtttctg
    67861 ctgagcctga attttttcgg cggggtgatt tgggcgggac tgtcgctcgg actccaaaac
    67921 tatgtgtttg actcgctccg tccgcaggag cgcacacgag gcgtagcctt ggccaatgcc
    67981 acgaacgcga tcggctgggg catcggagcg ttgaccggca gctggctggc caccatcctc
    68041 ccggctcagg tctcgctcgg ctcctgggca ctgaccccgg catccaacct gccgttcctg
    68101 ttctgcctct ccggagcgct acgattgctg attgccctca gcctgctgag aaccttcggc
    68161 gagccacgag agatcgggac gccgccgcaa cggcacctga tgtgggagtt gccgttggtg
    68221 aagcccctcg tggcgctgct cccctggaga aattctcgcg tcgcgcccta accgttcgcg
    68281 tcgccatgcc ttggctgact acatcccggc cggcttatgt tcccgttctt cctgcttaag
    68341 cttgtcgaac ccccgatccg cattgccctt cacctgctcc aacgagggtt gggccatcgg
    68401 tttcggcttt tcactcgcac agccggtcac gaggctcccc gccgcagcca atcctcccaa
    68461 caagccgatc acgcgcatcc ccgcaccgac tctgccgctc ctgtactcca tgccacaccc
    68521 cctttcgatg aatctatccg ctgacgcaca gccccgacaa agcattatgg gatagtgctg
    68581 cgccgcgctc aacatgaaaa cgtatccggc ccacacacgt tgactgcctc gggcaaagac
    68641 gttatcctcc gcgacaagat ccgacacctc ctgaaaggaa cccgatgtcg ctgcatgacc
    68701 gtttgactga agatctcaaa tcggcgatga agtcgcgaga ccaactgcgc atggacgtca
    68761 tccgcatgat taaagccgcc gtgcaataca aggaagtcga gctgaaacaa gacctggacg
    68821 atgccgccat gagccggatc atgaccacgc tcatcaagca acggaaggaa gccgctgagc
    68881 aattcgaaaa gggtaaccgg caggacctgg cgacaaaaga acggcaggag atcagcatca
    68941 tcgaaagtta tcttcccgcc gcgctctccc ccgatgaact ggcccggatt gtcgacgccg
    69001 cggtgaagga atccggcgct gcgtccctca aagacatggg gcaggtaatg aaagccgtca
    69061 tggccaaact cgccggccaa tccgtcgacg gcaaggtcgt cagcgatctc gtcaaggcgg
    69121 cccttcagcg ctagacgcga cgcccccctt ccggcccctt aatctcttcg tcgattctgc
    69181 cgatacggtt ctgacaggtc gcatcacatc gatgcaaccc taggcatgaa ccgctttcac
    69241 catgcccgat aggcaggtcc aggacctgct ctcacgcccc caaccagacg aggccgcgga
    69301 tgggtatcct gatcgtcgac gactctcccg accaacagct gctgctgaaa gccgtgctga
    69361 acaaagccgg ccatctggac ctgcttatcg cggactcggc gacagcagcc tacgaccagc
    69421 ttggcatcga tcagcccgag ccgcctcagc acactccgcc catcgatctc atcttgatgg
    69481 actttctcat gccgggcatc gacggcgtcg ctgccaccca acggatcaag agcctggaga
    69541 gcctgcggga cattccggtg atcgtcgtca cggccaagac cgcactgggc gatctgcagg
    69601 cggcctttgc ggccggggcc atggattaca ttaccaagcc ggtcaacagt gtggagctgc
    69661 tcgcccgggt gaattccgcg ctgatgctga aaaaagaaat ggattgccgc aaaacccgcg
    69721 aaatggaatt gcgccgcagc aatgccgaac tccagcaggc gctgcgggaa gtgaaggtgc
    69781 tgaaaggcct ggtgccgatc tgcgcctcct gcaagaaaat ccggaacgat caggggttct
    69841 ggcaacaatt ggaagaatat atccagcaac attcggaagc cgaattcagt catggtctct
    69901 gcaccccctg cattaaaaag cattaccctg gcgtttaccc agactagctg ctagcatcgt
    69961 cctacgacac actttcaggg agacactgac catgggtatc ctgatcgtcg atgattcgac
    70021 ggacgatcgt ctgttgatcc aatccatcct cgccaatgcc ggttacgaag aaacctttgt
    70081 ggcggagtcc gccgccgcgg cgttccggtt gctggggctc gacggccccc ggcaaatggc
    70141 gggccgggtg gatttgatcc tgatggatat cgtcatgccg gccaccaacg gcatcgaagc
    70201 ctgccggcag atcaaagccg tcgaacgcta tcgcgacatc ccgatcatca tggtcaccgt
    70261 aaaaacggat ccggtcgatc tgcagttggc ctttgcggcc ggcgccatcg actatgtcgc
    70321 caagcccatc agcaagatcg agctcctgac ccgcgtccgg tgcgtgctcc gcctcgtcca
    70381 tgaaatcgaa cggcgccgcg cgcgcgagca ggaactcctg gaggtgatgc gccagttgca
    70441 ggaagccaac cagatgctgt tgcgtctctc gtgcctcgac gggctcaccg ggatcaccaa
    70501 tcgacgccag ttcgacgact ttctggacca ggaatggcgc cgggccgcgc gcgaatcgac
    70561 cccgctgtcg ctgatcatgc tcgatatcga ctacttcaag acatacaacg acagccatgg
    70621 gcaccaggcc ggggatgaat gtcttcgcca ggtggcccag ctcatctcca gccacgtcaa
    70681 tcgccccggt gacttggtcg cccgttacgg aggcgacgag ttcgtcatcg tgctgcccgg
    70741 caccacggta gacggcgcgg cgcaggtcgc cgatacgctc cggcgcaaag tctgtgaagc
    70801 ggagatgaaa catccgaacg gcgatcccgt caccatcagt ctcggcttcg cctgcaccgt
    70861 gccaagccgt gtggccgcgc cgaccgatct catcagggcg gccgaccagg ccctgtacca
    70921 ggccaagcag gacgggcgca atcgggccaa gcaagccgga gtcctgacgc tcgtgcacca
    70981 cacgcataaa gattgatgac ctcccgccgt cgcgaccatc gtgtcaccca ctcgcgagac
    71041 ggagcggtcc tgctggactg agacgaccgg accacgccat ggcgaaacgc atcacgcaga
    71101 ccagacgaac cacctcaacc tctgccacac gccttccccg caccgcttcc cgaccgtcca
    71161 ccgcgctgca acgcgagcag gccgcgcgcc tgcgtgccga gcgggcgctc cagcgcgtgt
    71221 ccaaggaatt gcaacgccgc gtcgaagaac tgcaacgggc caaagatgcc gccgatgtgg
    71281 ccaaccgtgc caagagcgag ttcctggcca gcatgagcca cgagatccgc accccgatga
    71341 atgccatcat cggcatggcc gacctcttat gggaaacgac cctcactccg gaacaacgga
    71401 aatatctgcg tatctttcgc cgcgccggag ccagtctgct gagcctgatc aatgacatcc
    71461 tcgacctctc gaaagtcgaa tcaggccgcc tggacctgga ctcgatcgac ttcgacctgt
    71521 ccgagctgat cgacaaaacc agtgagatgc tggccttgcg tgccaacgag aaaggcctcg
    71581 aactcgtctg ccacctcgcc aacgatgtcc cctgccatct catcggcgat cccaaccgtc
    71641 tctaccagat cctgctcaat ctgctgggca acgcgatcaa attcacggag aagggctcgg
    71701 tggtcatgag tatcaccaac gatcccgagc ttcgaacgcc gggcgcgatt cgattctcga
    71761 tcagcgatac gggcatcggc gtcccgcacg acaagatcaa agccatcttc gacacgttca
    71821 cgcaagcgca cagcacgatt gcccgccagt acggcggcac ggggctgggc ttgtcgatct
    71881 cgcaacagct ggcagccctc atgggcggcc ggatctgggc ggaaagcacc ctcggcgaag
    71941 gcagcacgtt ccactgcgtg gtccagcttg gcgtgcaaac agccccgccg ccatcgaaag
    72001 tgtcgggcac gatgaacctg gccggactcc gctgcctggt cgtggacgat tacccgacca
    72061 atcggctgat cctgcgcgaa accctgttcg ggtggggtgc gaccgtggtg gaagccgaca
    72121 gcggcgagca ggccatggag gccttggaag tctcgctgaa gtccgacgcc ccgttcaccc
    72181 tgctcctgct cgattgccgg atgcccggaa tggacgggtt tgaggtcatc gaacgcatcc
    72241 atgccgatcc gaggctgagc aacctcacga tcatcatgct tgcctccgac cggtgggccg
    72301 acgacatcgc caagacctac gacctcggcc tgggcggcta cctggtcaaa ccgattcgac
    72361 gctccgacct tcagcaaacc atcagcatcg cattgggccg tagcaagagt ggcttctcgc
    72421 cttcgcctca aggcgccgct ccccaggccg caccccaagg acgatccctg cgaatcttgc
    72481 tggtggagga ctcgccggac aatcaggtgc tgatccagtc ctatctgaag aacaccatcc
    72541 atcgagtgga ccttgccgat aacgggcaga tcggcgtcgc aaaatttcaa aacgggcact
    72601 atgacctcat cctcatggat atgcagatgc cggtcatgga cggcatcacc gcgacacgaa
    72661 ccattcgacg ttgggagcag gaacagggcc ttcccgcagt tcaaatcatt gccctcacag
    72721 ccttggctct gaaagaagaa tcggccagga tcttcgaagc cgggtgcaat gcccatatga
    72781 caaagccgct gaaacgaacc acgctgctgg aattactcct ggcctacgaa aagacgcgag
    72841 cgcaatgatg caccattatg ctaaaccagg ccccgacaag atccccgtcc gtatcgattc
    72901 cagccttgaa gccatcatcc ccgggttcct cgccaaccgg cagcgggatc tcaccacgat
    72961 cgagtcctgc ctgaagcagg gcgatctcaa cacgattcgc atgcttggcc atcgcatgaa
    73021 aggcgacggc ggcgggtacg gtttcgatca gatcagcgtg atcggcgacc aacttgagcg
    73081 ggccgccatg gcacacaatc tctcggcgct acacgatcag ctcaacggtt tgaaggactt
    73141 cctggctcgc gtcgagattg tgtacgtcca ctgagttcag tgccctcgcc cgtcgctccc
    73201 ctttcctcca tttgcgcgac ctgctctctt acagactgct ctacccgcta aattgtagac
    73261 gacacaagcc aaacatatcc ggcgacaatg caggctcact ggggcagttt cgaggaatga
    73321 gggctgtgga agatatgtgc ggaattccat gagagacgga gaccgaaggt tgcagccgag
    73381 cgcgagcagg aggattggtc acgaaaactg gggcgagaaa gaaggctatg cggggtcacc
    73441 caaggagatg cgccgggttt aatcaggacg ggaggaacta ccctactagc ctcggatcgc
    73501 acagacgagg tttggtggag ggcaggggag ttgaaccccc gacccctacg ttgcgaacgt
    73561 agtgctctcc caactgagct agccccccac aactctcacg cggcattata cacaaccgga
    73621 gcgcgcgtct ccatacgaac ttacagatcg gcggaagact ttatccgggg ttgtgaaatc
    73681 ccgtcgccac cggtcctgcg tccgccaccg atacccgccg tccctccacc cgcagccgtc
    73741 cctccgcgta gagttgaatc gcgcgaggat agatccggtg ttcctgctcc aggatgcgag
    73801 cggccagagt atccggcgta tctccctccc gaatcggcac ggccgcctgg atgatgatcg
    73861 gaccttcgtc gaccccttcg gtcacaaagt gcacggtgca accggcgatt ttgcacccgt
    73921 ggtcgatggc ttttttctgc acatccagac cagggaacga cggaagcagg gacggatgaa
    73981 tgttcatcat ccgattctca taggccgaga ccagcaccgc cgtcacgatt ttcatgtacc
    74041 cggccaggag cacgagatcc acttcgtgct tctgcagcac ctcaaggacc gccttatcga
    74101 aagcctcgcg actgtccgga cggcctgcat acggcttcgg atccagccac acggcgggcg
    74161 cgccatgctt tcgcgcccgc tccagcccac cggcatcctg cttattactg agcacgaccg
    74221 caacctcggc cgacaacgtc cccgcctcga tcgcgtcgat gatcgcttga agattcgaac
    74281 ctcggccgga aaccaatacg ccaaggcgca ccaatcgagg tgatttaccc gacatactca
    74341 accacgcctt cgtccgtcgt ctgcgccacc atgtcgccga tgtgataggc ccggtcgccc
    74401 gcttctaccg ctttggccat cacgcgatcg acgtgttccg gcgccacgac cagaatcaac
    74461 ccgatgccca tattgaagac gcggtacatc tcgctcagtt ccactccccc gcggtcctga
    74521 atcgtctgaa aaatcggcag caccggccag gatccgcgac gaatgcgcgc cccgcagcgg
    74581 gccggaaaga ctcgtggcag attctcggtg attccaccgc cggtgatgtg cgcgatcccc
    74641 ttgatcgggc aagcctcggc caacgaaagc acctgtttgg cataaatccg cgtcggcgtc
    74701 agcagggtct cacccagcgc accaccgagt tccggcgtca cactctcgac cgtcagtcgg
    74761 cttttctcaa acaacacctt gcgcaccagc gaaaagccgt tgctgtggac accagtcgag
    74821 gccaacccga tcacggcatc cccgggaaca atctgccggc cgtcgatcat cttcgggcga
    74881 tcgaccacgc ccacagcaaa accggccagg tcgtactcgc cttccgcata gaacgagggc
    74941 atctcggcgg tttctccacc gatgagagcg caaccggcct gacgacaccc gtcggaaatc
    75001 ccgcggacca cggcctcggc cgtctttaag gagagtttcc cggtcgcaaa gtaatcgagg
    75061 aagaagagcg gctctgcgcc gctgacggca atatcgttga cacacatcgc caccaggtcg
    75121 atcccgaccg tgtcgtgttt gtccatgagg aacgcgatct tgagtttggt cccgacgccg
    75181 tcggtgcccg acacgagcac cggatcctgg tagcgattcg cctggaatcg gaacaggcct
    75241 ccgaagccgc ccagatcggt catcacttcc gggcgaaagg tcgcccgcac atgcggcttg
    75301 atccgctcga cgaattcatc cccggcatcg atatcgactc cggcatcacg ataggtagtc
    75361 ataagaaggg cgcatcttag cgatgcagga gagtgttggt caacatcgga gatcgattgt
    75421 gatctgcact atgatgcgcg taacattgaa ccgtgatcgt ccgagccccg ttcgatagga
    75481 gcacgttgct caaaacggtc gcccagcaag gccgcaagga gccgacgcga gtgaggcgta
    75541 ccccaggggt acgtcgcagg gagaggcgcg actgagaacg ccgctggcgg acgttttcag
    75601 caacgtgcct acatctcggg acgcatctcg acatccaaca acttaatctt gcgatgcaag
    75661 tggctgcgct cgatctgcag gtcttcggcc gtacgggaga tgttccaatg atgttcacgc
    75721 agcttgcggg cgataaattc cttttcgaac gcattgcggg catcgcggag cgagtcgtaa
    75781 tgcctcgtca agagggaatt gggcgcctgc accccggcgg cctgttgcgt agcggacagc
    75841 tgaggacgca cctgcaacgc taccgacgca tgcgaagctt cgatcacagg ccccggcacc
    75901 atgatcatga gccgctcgat caggttgcgc agttcgcgaa tgttgcccgg ccattcatac
    75961 tggatgaaca cgtccatggc ctccggcgag acttgcttga ttttcaagcc ttgctcctcg
    76021 gcatgcacat ggaggaaatg tttcaccagc aacgggatat cctcacggcg atcccgcaac
    76081 gtcggcacca cgatcggtac cacgttgagc cggtaaaaca ggtcctcccg gaacgtgccc
    76141 ttctcgattt ccttcagcag atccttgttc gacgccgcca ggacccgcac atcgaccttg
    76201 atcagtttgg tcccgccgac ccgcgtaaat tgctgctctt gaagggcgcg caagaccttg
    76261 gcctgcgtac tcaggctcat gtccgcaatt tcatccagga acagggtccc gccgtcggcc
    76321 tgttcgaact ggccccgctt catcgttgtg gcaccgctga aggacccgcg ttcgtgcccg
    76381 aacaattcgc tctcgatcaa ggtttcaggg atggcggcgc agttcaccgc cacaaacggg
    76441 cggttcgccc tggcgctctg ttgatggatg gccctggcga cgagttcttt accggtcccg
    76501 ttctcgccgc cgatcaagac ccggctgttc gtaggccccg ccgtttcaat aatctgcttg
    76561 agttgccgca tcgccggcga ctgcccgatc aactcgaact tccgctccac cttcgtgcgc
    76621 agcgtccgat tctcctgctc aagccggtgc tgatccaagg catgtttgac ccgtagcgtc
    76681 acgttttcga gcgagagcgg tttctcaatg taatcgtagg ccccgagctt gatggccttc
    76741 accgccgtct cgatcgatcc atgcccggac atcatcatga cctgcgtctg aggcaccagc
    76801 tcacgcaagc gccggagcgt ctccaaccca tccatgtcgg gcatccagat gtccagcatc
    76861 atcagttcgg gaggactcac ggcacataac ttgagcgcct cgatgccgct cttggccacg
    76921 ctgacctcat atccttcatc ctccaggatg ctgctcaagg agttcaggat ggagacttca
    76981 tcatcgacaa tcagaatcga agcagacatg tcttctccac actacgcgtc gcgggaaccg
    77041 ttcacaccgg caactcgaac gtgaacaagg cccctttggg ctgatggttt ccggcgtgaa
    77101 tctggccgtc gtgatcggtc acaatccgcc gcacgatcgc cagccccagc ccggtacccg
    77161 ttttcttccg tgaaaaatag ggcacgaaca atttctcttg atcctccggc gcaatacccg
    77221 tcccttcatc cgccaccgtc acgactgcgc gacgctgttt cgtgtcgtac cgggtcgtga
    77281 tccacagatg tcccttgtgg ttcatcgcgt ggatcccgtt atcgcacaga ttgaccagga
    77341 cacgtttgat ctgttcccgg tcgaaattga acggaggcag atccgcatcg agcgtcacca
    77401 cgcattccac ttcgcgatgc gcgctgtcat acagcgccac cacttccttc accacatcat
    77461 ccagcgagtt catcgtcatg tgcggagccg gcatccgcgc gaacttcgag aactcgtcca
    77521 gcatttgctt gaggctgccg acctcattaa tgatcacttg cgtggactcg tcgcaaatcc
    77581 ggtcgaaatc cggagcctta tcctgaaact tttttcgcaa ccgttgcgcc gagagctgaa
    77641 tgggcgtcag cggatttttg atctcatgag cgatccgctg cgcgacctcc cgccaggccg
    77701 ccgccttctg cgccttgatc aattccgaga ggtcttcgaa aatcagcacg aacccgaggt
    77761 ccttgttcga ctcatccttc atgcgggagc aatgcacgcc gatggtcaga aaccgcccct
    77821 gcaaatccag ttgtccttcg agcgcgatat tgtctcgctg atcgaccagc atccgatcgt
    77881 agacggactg aaacagatcc agcttgtatt ccttgaacac ttcgttggcg gaccggcccc
    77941 gaaaccgatc ggcccacaac cccagcatac gctccgcaga gggattgaac gtggtaatga
    78001 tcccctgccg gtcgatggag agcagtcccg cggcgatcgt atccaccacc gtttcaatgt
    78061 aggcgcgccg acgatccaat tcgaggttgg attgccgaag cgagacgttc gcttcttcca
    78121 ctttcgtttt gctgctctgc agatcgccgg tcatgcgatt gaaggattcg acgagggtgc
    78181 cgatctcatc cgtcgccttg gcttcgatgc gcacggacag atccccttga gcgatcgcct
    78241 ccgtcgcctc ggccaaccgt tgaatcggca cggtaatgcc gcgcgccaca taaaacccga
    78301 accaggtcgc actgaacaga atcatgaccg tgatgaccgc caccagcaga tacgctcccg
    78361 ctttgatggg attcttcatc gccttcatct gtttgtattc ggtatattga cgcccgatgc
    78421 tctccatctt gccgagcaac gattccggca cataggcatc gaccaccacc accccgtcga
    78481 tttctccacg gcggatgctg gaggcgatcg gtacaccggc cctcacaagc cgtcccgtct
    78541 gcgcttcctg gacggacgtg agttcctgct tgccgttgat cacctgtagc accagttggc
    78601 cgatcggcag atcgagcacc gccaccggca cctctggatc cagcgacttg gtcaacgttt
    78661 ccatcttcga ggaaaacacc tcgatcccgg ccgtggcata ttccgcgcgc ttccgggcga
    78721 tcgccgcaac cagcaaatcc cgctgctcgg gcaagagcaa ctcctcgcga aaaatctcgt
    78781 ggctgatggc gcgtgcgctg ttgatcgcca gggacacatg accggcatgg tgcatgcgcg
    78841 cgacctcgta agaatccttc atcacgtgct cgatctggtc gttgaaccag acatcgaccg
    78901 ccttgttgac caggccgctg gccaccaggg ccaggagcac ggtgggaatc agggaaaagc
    78961 cgataaaggc ggcgaccaac ttcgcgcgga accccgaccc aacgagccga tgtcgccgtt
    79021 cgaaataggc tttgatgaga ttgcgcgaga ggagtagcgt gaggacgaca aagccgatca
    79081 aatcgagatt gaccagcagc agcacgaagg cataaccggt actgggcaga aaggagtccg
    79141 actcctcccc gaccggcacc gccatttgag cgtagtaaaa agtgagggcg aggcagggca
    79201 ggagcaggac cagcacgatc cacactggcc ggacatgacg cttccgccgc tcgctctcaa
    79261 tggaagcagg cgtcgacggc tttttctcgc gcagcacccc cggggggatg agcttcgtca
    79321 tatccgtcga ttcaggcatt ctcgatactc cgactcacgc ccggcccatt cgatcctgcc
    79381 tcactataac caattccagg tggagtctca aaagagggcg cgcgcaaccc aacccttgct
    79441 tcatcgagaa acgaccggaa gcagggcatt gaagaacaca gtgtcacccg aaggaaggac
    79501 cgacaccgca aagcaggacg gcgcgctgaa gcaactcacg aggttacggg gtatgaagcg
    79561 tcgatttccc ggaggtcaaa ctgacgaatt tcatcctcaa ggcgtagagc atgtccttca
    79621 ttacgacttg ctcgtccggg gtgaggttgc ccttcgtttt ttcttccagc atggacagta
    79681 gatcgatgat ttctttggct tgaggaaggt tcagtggcat ggatggctgc tgaggatcga
    79741 gttgctcccc catcagcatc agagctgaac tccccatgga gatcacaaag gaggaaaagt
    79801 tgaccggcaa atgccctgag tgcgtatgcg aatcggcatc atggtcgtgg tgaggcgcgg
    79861 cggcagaggg ctctgctgcg ggaggcgtcg ccgcctgggc cggtggggcg gcatcttcac
    79921 cccgtcctcg acgatcgcgg atgacgaatc cttggtcctt gtcatcaccc ataaaaaagc
    79981 ctccgatcaa ccaatggaaa atgcagggcc gtaccctacc acagggcttg aagaggcgca
    80041 agacgagagg ccccgttgaa ggtgccgaaa aactcagtta gagtggcgcg acggaggggt
    80101 tcgcatgtca gcacgtttcg ataacgtcgt ggccatcatg gcccggcttc gggcaccggg
    80161 cggatgcccc tgggatcgca agcagaccca cgaatccctc aagccctatt tgatcgaaga
    80221 aacctatgaa gccctcgata cgatcgaccg gcaggatttc gccaagctaa aggaagaact
    80281 gggcgacgtg ctgctgcagg tgttgtttca cagccagatc ggcactgaag caggcacgtt
    80341 caccatcgac gacgtcttgg aacagttgag cgacaagctc gtacgccgcc atcctcacgt
    80401 gttcggtgac ggggcgacca gccagtcctc acttaattcc gaccaggtcg tccatcggtg
    80461 ggaagatatc aaacgggccg aacgcaagaa cgccggcaag ccggattcgg tcctgcatga
    80521 cattccccag gcccttccgg ccctgctccg cgcctatcag acgcaggtcc gcgcggcgcg
    80581 ggttgggttc gactggccgg acagcccgca gggcctggcg gccgtgctgg ctaaagtcga
    80641 ggaagaaatc ggggaactca agcacgctct caccgacgcg actgcgcgtc gctccgtggc
    80701 cgaacccaag ccggagccga ccccggaaat ggccgcggag atgggcgacc tgctgttttc
    80761 cctggtcaac gtcgcccggc acatcaaggt gaatccggaa gagtcgctcc gtctcgccac
    80821 gaaccgattc accgcgcgat tccagttcat tgagcgcgag gcggctcgaa gcggacggag
    80881 cgtcaacgac ctctccacgg tggaaatgga tcactactgg gaagccgcga agctggtgga
    80941 atcgacagca cccgcgggca ccgtccctcc cctcgtccct tcgacagagc cgaccaggac
    81001 tcatccatga gcacagagca ggatccatac gaagaaggca agcgagccgg gcttcatccg
    81061 ctcatcgtcc tgttcggggc cattctggtg atgtggctgc tgatcaacct ggccgttccc
    81121 agcggcaaac aaaagcaggc cgccggtccg gagatttcgc aggtgacgac cgaagatccg
    81181 gatgcggccc cggtcatgta taaagtccag tcgacgttga cggagtttaa tgccgtcagt
    81241 atcgtggtgc cggcgcaggc gacgaccagt caggtggtgg gcctcctcaa acaattcaag
    81301 caggcccggc tggctcagag tctcccctcc atgttgccgg ccaccagccc agggcacaaa
    81361 ctcggggacc aggccgtggc cgatctgtac attttttcag accccaaata tgcaactccg
    81421 gaagcggtcg gcaccctggc tcgcggcgcc cacgccccgg gcaacctcta cccgcaggcc
    81481 atccccttcg aagaagcgat ggaacacgtc cttgggcact atcgcatcga cctgaacgac
    81541 accagcaatc ccgacaccgg atcgctggga tttgccgacg attccggagt acacagcaga
    81601 caatatcggc ggatttttta agcagccgcc tcgaaaatcc tctcccagac tgccttcgct
    81661 atccccagga ccgcattaag caagacgtat cttcctaagg gttttcatcc catacagcat
    81721 ctagtggtct ggatagaggc caccccaatg cctaaaactt gtgcaggccg ctcaagggcc
    81781 gctctactga ggggcttggg cgaggaggcg gctgatattc acacgcttat ccacagattc
    81841 tgtgcgttgg ggaaatggcg acagaaccgt caactgacaa gtaccggaac gctcatcggc
    81901 tgggcgcaga aatggcttgc ggcacgtcac aggaggctgg cttcggagta acggatgaaa
    81961 tctggacgag cagtggaccg aaggtgacag agggcgcaat cgtcagacgg ccggaggact
    82021 ggccaccagg ccgaccaggt tcttgatctc cgcccgcaat gtatcctgct ccgcttggac
    82081 ccaggcctca agttgcggga agtaatgagc gagcgaaccg gtgaaggggg ccaccaacac
    82141 atcaccgcgt tctttcaacc acccttcggc ctcggcaata cgaaccttct ggtcaccgat
    82201 cttgcgggac agaaatttgt tcagcatcaa ctgctggccg gggctgccag tgacagtgcc
    82261 cagcaacttc tccttcacat agtccaactc ctccgcctgg gcaatcttca ctcgaacggc
    82321 atgggttgcc gcccaggtcg cccgttgaat cgagtagtcg taggacaaca ccggctcccg
    82381 cggctcgcga aagggaccgg tcacatcgga gatcaccaat tggtcgttca gcatctgggt
    82441 attccttctc ttcccgctcc catcgtcatt gaaaccttag accggcgccg cctgtaaata
    82501 ccggagcagc ttcgccgccg tctcagcgcc gtctcgaata caatcgggaa ggcccacacc
    82561 gcgatagcct gcgccggtga cagccaggcc accgaatcgc gagagcgccg cctccagctg
    82621 cgcgagccga tcgaggtgtc cgagggtata ttgaggcatg gcccgattcc accgattcac
    82681 ctcgacatag tgcggtgtcg ccagcagccc cacaatggag gccaattcct cgcgcacaca
    82741 ctggaccaag gcctcatcgt cccgctgcaa gatggcttct cgcccgaccc cgccgagata
    82801 acatcgcacc gacacatcct ccggcggtgc tcgatggggc cacttgaggg aggtccaggt
    82861 cgcggcaatc agatcacggc cctcgattcg tggaaccacg aagccgaagc cttggagccg
    82921 gttccccact gcttcggcag gatagatgag agagatcgtg gctgtcgagg catagggaat
    82981 catgtccatc aagccggccg ccatcggcgt taacggccga acgagttcag cgctgacata
    83041 agccggtgtc gccaagacca gcgcttctgc cgaaagcgcc gtcccgtcgg tgcaaatcac
    83101 atcgtacatc cagcgaccgg cctggtgcga acgcacccgc aacgcctccg cttgaacgcc
    83161 ggctttcagc accccgccgg cctgctggat ctgcgccgtc agcccagcca ccaaatcggc
    83221 caggccgttt ttcagcgtca caaacatcgt gtgtcggggg cctccgccgg aaaccttctg
    83281 cgcccgtgca cgacgggccg ccatcatgcc gcgaatcaca ctgccatgcg cctgctccaa
    83341 ctcgtagaaa cgcgggaacg tcgcccgaag gctcatctgc tccgcatcgc ccgcataaat
    83401 gccggccatc agcggctcca tgacccgttc gcaggcatgc cgtccgaacc ggcgacggaa
    83461 aaacgacgcc agcgattcgt cgtcggtgga ccgacgcggc ggaatcagca catcgcagcc
    83521 catacgggca agatcgaccc aggataacaa cccgctgcga aaaaacggcc ccagctgagt
    83581 cggcgtgaat gtcacgagcc cttccggcaa ctcgtgcaac tgcccgcctc ttaagacact
    83641 cgctttcttc tcaaccgggt tggtgttgat cagttgatcg gctagcccaa gacgtcggca
    83701 cagctccatg ccccagggct tttgcgagag gaacgagtcg ggccccgctt ccatgaccaa
    83761 ctgcccgacc cgatgcgtga gaatttttcc gccccagacc ggcgccgcgt cgaggatgat
    83821 gcaggtgagg gccatatcag ccgcggccgc ctgctcctgg agagcgaagg ccgtggagag
    83881 gcctgagatg cctccgccga taatgacaac tgaccgtgga gtgcgcgcca caggtggtgc
    83941 cgctaatagg gagtggaaca atgctgtgac gctatctgaa caattcgagc gtcaggataa
    84001 tcgacgaatg attcccgcag gatgttcaaa aaggtcctgc agcgcggccg cagcaagcga
    84061 cgaggtgagt cgtacgtggc tgggtacgtc gaacctcgga gcgatgcgag aacaaagctg
    84121 caggcctttt tcaacatccg cctaatgaac cagcgaggac tcgtgtgcct ccaccaccga
    84181 catcagaatg tcgatcaatg gagccgaggc gttcaacatg agaatacgtt ccagctggag
    84241 ccccttcgtc agagccagct gcttgagctc aatatcaata tcgaacagag tttccacatg
    84301 gtcacagatg aagccgatcg gcgccaccag gacatgatga tgaccttcct gcgccaattc
    84361 acccaacgtg tcttcaaccg atggaccgag ccacttttcc ccggagcgtc cctggctttg
    84421 ataggcaaac cgcgccggct gagtcccgag ttgcgcgcag accgcctgga cggttccctg
    84481 tacttcctca ggatacggat ccttcatggc caccacgcgc tcgggcaaac tgtgggcggt
    84541 aaacaagacc ggcacctgtg cacgcacctc ggccgggaat cgctccaacg tccgacgaat
    84601 attctcgacg atggcggcga tcagcagtgg atggcgatgc caactcgtca cgaagctgat
    84661 cggagtctca ctggcgagtt cagcccgcgc ctcctcgacc tttttcatgt aggcgccgat
    84721 gctgagcgaa gaatactgcg gggccatgca caacccgatg atgcgttccg gtaaggcgtc
    84781 gagcaattcc gcgtaagttt ccttgataaa cggatgccag tgccgcaacc ccacataaca
    84841 gcggtaccgc gcgtcgcccg accgattcag ccgctgttcc aatgcccgcg ccacttcgcg
    84901 cgcgatatcg agcaccggag atttcccgcc ggtcacacga tagcgctcgc ggatttcctc
    84961 aaccagttca ggagaagtcg gccgtccgcc tcgaacgtcc tgaagatacg gctccacatt
    85021 ctccagacaa tcggggccgc ccatagccat cagcaagacg gcaaccggtt gtggcgtcac
    85081 agacatgctc accgcgtact cagtttatgc accagatcga tcgtggcggc gacattctcc
    85141 acgggagtat tcggaagaat cccatggccc agattgaaaa tgtgacccgg acgccctgcg
    85201 gcccggcgga gaatgtctgt cacgcgccgc tcgatctcgg agatcggccc gaacaacgtc
    85261 accggatcga ggttcccctg cacggcacga tcgtagccca ccatggccca ggcctcgtcg
    85321 atcgggatgc gccaatcgat accgatcacg tcgccacctg cctggcgcat ggccttgaga
    85381 atggccgtgg tgccggtgcc gaaatggatc agcgggacgc cttcgtgctt gagtccctca
    85441 aaaatcagct gcacatgcgg catgacgtat tcggcatagt ctccggccga cagacacccg
    85501 acccagctat cgaacagttg cacagcctga gcaccggcct tgatctgacg acgaagatag
    85561 ccggtaatca cgcgcgcgaa tttatccatc aagcgatgcc aggtttccgg ctcgcggtac
    85621 atcatctgct tcgtatggat atagtttctg gaacttccgc cctcaatcgc gtaactggcc
    85681 agcgtaaagg gcgccccggc aaagccgatc aggggcacgc ggcccgccag catcttgcgg
    85741 gtcagactga tggcatccat cacgtactgt agtccggtgt catcaatgac cttgaggcgg
    85801 tctaccgcag cccgatcccg cacaggattg tgaatgatcg gcccctcgcc ctccgcgaac
    85861 tccaagctga gccccatggg ctcaagcgga agcagaatat cggcaaaaat aatcgcggca
    85921 tcaagcgcaa accggtcgat cggctgcaac gtgacctggg ccgccaactc cggcgttttg
    85981 cacagctcga gaatggaatg cttttcccgt aaccggcgat attcg
//
LOCUS       DAORRQ010000010        80904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_10_length_80904_cov_238.644510, whole genome shotgun sequence.
ACCESSION   DAORRQ010000010 DAORRQ010000000
VERSION     DAORRQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 80904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 80904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80904
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10_length_80904_cov_238.644510"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..679
                     /locus_tag="PK224_05015"
     CDS             329..679
                     /locus_tag="PK224_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15125.1"
                     /translation="MRRKHVQFCMKWKITYPLHSSSIRGGRGDFAWLKKPETAGLFFQ
                     SLEGFLLSLPILGVACVIHRPGYVARYQDRYKEALWYMCKTAFSILIERAAKFADEQD
                     RFLEVVFESGQDSF"
     gene            complement(927..2237)
                     /locus_tag="PK224_05020"
     CDS             complement(927..2237)
                     /locus_tag="PK224_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRB15126.1"
                     /translation="MAQSSLDVYQKLIDECQSVQKTEAICLIKRADDLIANTSPILQQ
                     RLMEQKRTASRFNIFNVMGLAGLEDYHSSFLGHLLDPRASHDQEDMFLRGFMETVIGC
                     HPIPQDSINAIVMREQCVEPFGRLDITIYWPSGQILVIENKINAQEGEEQIARYLNWL
                     NLQPEPRIGEHLLAFLTPTGRKPKTSTSEHVRCISYLGLGDWLSTASSSVPGRLQVIL
                     TQYIETCYNFSNDIRRLNMTDELTVLLRQPDKLEVALDIFKALEWLQKDIYKEYWRNV
                     RDILQNQLGEGGNDEKWEVYMAEDVFDTYSKLGLVWRERAEGILLFSVLFECLAGKPT
                     YYGIMRGKPIGAESLNEQDKILSGTLTKELNFSKSIWWAGYQNLSKVNLTHFNGVNKQ
                     NVLSLHKDNLNDDHPLARMTASVLWGLFLRCKIQLETLNEQYPY"
     gene            complement(2563..3819)
                     /locus_tag="PK224_05025"
     CDS             complement(2563..3819)
                     /locus_tag="PK224_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRB15127.1"
                     /translation="MASMKASTSPPFNGKFLFTPEDLQQEFATLLNKADSLKIAAAWA
                     TLGVHVDMLKNAKIPIEAIVGKAFNITHPDAIAALMSLGKVYVDERKTDFGLFHPKVY
                     LFQTGSQYSIIIGSPNLTCGAFTVNAEVSVSFKLDHKSALPLIEHFRNLGRAATPVTT
                     AWLKRYRKQYKPPKSGILKKILKSQSPRENQSSASASTVGDLLTLTWQDYYRLLQKQA
                     TRADGKSSLLDKDESYLKTLDVIRPIIQKRFDQLTKEDFRYLIGGNETYPNTGYFGSI
                     AGAIRAVPALMRDLRLRRDITKLIPRLIRARNTQSALAIARSLFLRLVRVKGIKSAVA
                     TRFLVLSRPDLFISVNNESVFRLSRLFGIPVSKIKTWEGYELALAKLWQSRWYHSHRP
                     RNVTEAKVWDARAALVDIYASEYEWS"
     gene            complement(3858..7772)
                     /locus_tag="PK224_05030"
     CDS             complement(3858..7772)
                     /locus_tag="PK224_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRB15128.1"
                     /translation="MGFPLVEKPKRLVLPRGLMPWTGFFKRKSDKSDGSPVLSIAATA
                     DGLRVDFAKPLVEATSDSLFEAASEASAQEAFLGMYVAQLGQEGLCQIDTTGVTLPWP
                     QVYSLAKSPEHTGVVNELNLPSEINLNPIIDCRGILSDTDFELKIVGWAQGSQGIEVQ
                     RLVGAYATVHDNAVLLPEAAWAVANAIAEFNGRPGNERTRHINELAWGRIRQLATDAG
                     AFFRSPYLQTTVVLTPLTLRLPLTKEEVLGERVLTVAPTFDEAPEEWLSAFDGFREVQ
                     DHYDLISDGRRVRVVLSEPVKRVLRAIKREMPTRRIAGSRAEKLIHNPWAYLGDTAQS
                     VIREEEFLKDKAAVGALATIFDIRLRTENGRIERVDLVISEVFEDGCGRTTTKHFGGP
                     SELGVFVAKLRQAISDERQLFAWDEFDLTIDGDSTMQLEEARQIYSLWKQQPSKRIGF
                     DEVYALADYSERIEGIGIAKPIYVPVFQKSSDEEEPKPGWIPNDLTPMVAVSLAGHEG
                     RLLIPLTKEWVEDFDKEVKKAESGAVPTVTNDSLPTPIETGQARALVDAFKSMLAAQQ
                     TVNVSKSLKGTREKREKERLLVKLNFYGVDYAEARKTLLSVDEDQQPRLPECLRPSVE
                     LKKHQRFGVAWFQHLVSRAPADCRGALLADDMGLGKTLQLLCLLAQHYERNPEAPPSL
                     ILAPKSLLDNWKKEIERFFTPSFPESLVLYNGELNKRKQPLGLIDEQLQTRGVTELLC
                     PGWLGNAKIVITTYEVLTGYEFSFAKQPFAFIICDEAQRIKTPGTQVTLSVKKLRADF
                     RVACTGTPVENSLADLWCLFDFVQPGLLKGLEEFGQTYRRPIESDTPELLAALQKLKE
                     TIEPQTLRRTKAHIASELPLKLFAHAKLNDALQFVPDLKDDMRLEIAMSDHQVALYRG
                     GLKRLQEANEERDGRRRSLLSFEAFHLMKAVCAEPYCVPGKKFLVDERGIDVHLLNSR
                     KMAWFLSTLKQIRDSTNGDKVIIFTEIREIQLALAYFIRQTFGLKPFTINGDTEGRQG
                     YIDRFTQSDGFNVIILSTLAAGAGLNVTAANHVFHFTRAWNPAKEDQATDRAYRMGQT
                     KNVFVYCPTMVTDAFPTFEVRLDDMLKQKAKLADATLEESAIVGMLNGVGADIGIGEL
                     VGDGTHGQTLQKRVLTMDDVDRMDGYTFEVFCKLLWEKRGFISNVTSKRGGDGGIDVI
                     AQLGMEGQLLQCKTSLHSEIGWDAIKEVVAGAALYQARFSGTRFSKVAVTNQRFTSGA
                     REQAEANRVNLIQREELSDMLKKYQVINLDLDTECNSGKDVK"
     gene            complement(7718..9454)
                     /locus_tag="PK224_05035"
     CDS             complement(7718..9454)
                     /locus_tag="PK224_05035"
                     /inference="COORDINATES: protein motif:HMM:NF026949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EH signature domain-containing protein"
                     /protein_id="HRB15129.1"
                     /translation="MLSGNDAVRSLEQALDSALASLHIPMVEFGRPAEIEKASKQAQI
                     VFQGIATARPSRQDASAAVFSFMRGESLTDHQYDLAASALCEPFRELNNTCVVGDSRF
                     DSLLNHYEFQAKSRNLWRLTWYGLLSSYFTFDMGKASDSEREGWIKLRNMLRRTWPDI
                     DTDGAVAIVPDWIKVMRSESQLLTAAAVDKYSVSYLNGETESINRLAEDLGIPQTSWF
                     WHSLVLGAVRKCCDFTCDTDFKARIPRLLELISTRPVFRDEALEILLERYHRSSSREP
                     HPQLRDFVVHKNVWRNPKLKAAGMATAWNRVSEPVWRMVLGWVNEANLKLFFELLAGR
                     SGSDEGRLEFWGRYLHQITWTRLVFGSETMELAHSNQEIRSLLAQEEGTYATLTLNKD
                     VDAFMMQIGDYLAVEFSKKPNACYVYSTRQLRFDSNARRYAGSTGDLKYGFYDGAQLQ
                     IIHVLGWEQKAEDKLKSLGIYPDKHSIGGVGLVGGKLADRIGQPATSNNRHSIGAIQT
                     DDAPKRAKFSIDNLRKLVSNYSEAHIKDYRTNGGRLWVEDSQQHASLCTALKRWDFRW
                     SKSRNAWYYPED"
     gene            complement(9448..10155)
                     /locus_tag="PK224_05040"
     CDS             complement(9448..10155)
                     /locus_tag="PK224_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB15130.1"
                     /translation="MLSRVVLKRGFKTEGESPFWISFSDLMTALMMLFLVVMAVTLIA
                     VTKNITVEEQRKIQRESDIKELMSRINEKSRIFQDVRVDESTYRINLGEKVLFDSGRA
                     DIRPDDARFLREYVPLLLDAQNSDIGRQWIRRIVVEGFTDQDGTYLYNLGLSLDRSRQ
                     VVCVLFAPTVSAEIPLADAQKRQIRDLFLVGGYSFNSMKTSKKESRRVEFKIDFWALD
                     EEKPADSPISDKELGRC"
     gene            complement(10158..12170)
                     /gene="zorA"
                     /locus_tag="PK224_05045"
     CDS             complement(10158..12170)
                     /gene="zorA"
                     /locus_tag="PK224_05045"
                     /inference="COORDINATES: protein motif:HMM:NF033916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage ZorAB system protein ZorA"
                     /protein_id="HRB15131.1"
                     /translation="MNAQSLLALLEQVHPSFGTLTGVPLYIFLLVLALAGAFFVGYFL
                     QGIRVAWQLWTAVRGIRVLSASKKAVAPEEIAKLLRNEPFWHLWAEYADTLHPLAKAS
                     SGAVTLFEVRATMPAEIFFTRDVLVDSRMFDDFVRHLPGVLTGLGIIGTFAGLLNGLS
                     QFNPSTTSTAVAGLKPLLDGVAHAFIASAAAISCAMIVLFIEKLVLALFYGKVEELNQ
                     VIDGMYHTGAGEEYLARLTHSAEMSETHAAQLKEALVEDLKQMMTNLVDRQIEAQKES
                     TQALGNQLGNAILEGLQKPMQDIGEAVKRASGEQGSAVHGMLESVLVAFMSRLEDTFG
                     GQMQSINESMERSMGAMASVQVSLQRLLENIEKSTENATLQMSQKLEDAMRQAAANQQ
                     LMTDQMREFVQEFRRLVTDEQAKSKNIMDETMSNVLIQLSTAMEHLKHERNASVKEES
                     GRQEQLKNRTTELVGGLSSQLDTLLRSVAEQVKSTQRNIDAINAASTRAIDGMNQGAL
                     TMGSAAQRFETAGNSVSAVFQRSEMVADQLQATATTLQSAALVIKQGYELYDNTRKTV
                     DSHVQMLTALIENAKREVGFTTQMIADLERVVGQLRIAETQSQQYLEKVNGALAKAFT
                     DFGSQMSHQVASTIKQTDTHLGNGMNQLTGVIQELGMALSKIPRNR"
     gene            12764..13012
                     /locus_tag="PK224_05050"
     CDS             12764..13012
                     /locus_tag="PK224_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15132.1"
                     /translation="MKTDDTKTETLQFKVTENERKLIEKCAAEEGTSVSKYVRGAVLM
                     SMVMDGKAEAIKIVAREVGEKTFAVVRQKLARPTQEGR"
     gene            13158..14168
                     /locus_tag="PK224_05055"
     CDS             13158..14168
                     /locus_tag="PK224_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB15133.1"
                     /translation="MESTFTLTVDWLAFTVLASNPQETMKVLGGDWSRAKGGFRGYPL
                     SWIKSDGTQGVGKLGTNAPRRPNEIHVDLSGGLVSVLTLEQIRGLLRWVHEQQGHVTR
                     IDCALDDRTTSVPMATIRQAVTDGHCVTRAAQVRQIVSNLTRGTGATTGETMYFGSPQ
                     SQTLLRIYDKRLEMQSKERENWQEFGVRWELEFKKDRAQLCARALASLDETDWKECVV
                     GLLRTYVNFRQIPKDPEEEERYRAPMLEWYVLLTEGFQKGRLAQEKQVQTLQNVKRWV
                     SDTLTPMLAVICATPGGEEWLQHEIVRGISRWKDRHRNLLKQPPNRFHPSSAGGNAGS
                     PC"
     gene            14211..14522
                     /locus_tag="PK224_05060"
     CDS             14211..14522
                     /locus_tag="PK224_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB15134.1"
                     /translation="MLTTKELSAWLNIKSSTLYLWVSQNKIPYCRIHGLIRFEPDAIQ
                     AWLRGFGAHSVKPLPLPTRQDSNDVENLIAAAKRAVYTSGHGETITPSPREKEEQHGA
                     R"
     gene            14509..15579
                     /locus_tag="PK224_05065"
     CDS             14509..15579
                     /locus_tag="PK224_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB15135.1"
                     /translation="MGLVKRGTTWWMSFTYQGQQIRRSTGTADKRMADAILGKVKAQI
                     IEGRFFEKPKEESRTFSELMDRYSTEHAARRANSRRNGTSVTHLRAYFGNPKLSQITP
                     KHIVAYKNKRYADGVKPATINRELSTLKKAFNLARREWEWCSDNPVCRVSMERENNTR
                     DRWLTLEEEHCLLQAALPWLRELLVFAIHTGMRMGEIVSLTWAGVDLNRRSVTVFRSK
                     NGERRTIPANQTVLRLLGEKHAVRQTVNNLVFHSQTGTQLDGSNIRRCLNSALKTAKI
                     HDLHFHDLRHTFATRMVQAGVDLYKVQRLLGHKSPVMTQRYAHHYPESLRDGVEVLDV
                     RRPISTNLAQPLGRIGEVSLTH"
     gene            complement(15815..16195)
                     /locus_tag="PK224_05070"
     CDS             complement(15815..16195)
                     /locus_tag="PK224_05070"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRB15136.1"
                     /translation="MPDRLTILLDQNVPQAVVSWLKLLKPTWAVFHTGDVGLSGKADE
                     EVFAWAQQQTAVVVTFDEDFADGRSFPAQRHHGIVRLRVWPTTIEETEQALGRLLSEV
                     TEAELTTSLIIVDRSRIRVRTRPQ"
     gene            complement(16188..16409)
                     /locus_tag="PK224_05075"
     CDS             complement(16188..16409)
                     /locus_tag="PK224_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRB15137.1"
                     /translation="MDDRIVVDPNICSGKPTVRGTRIMVKNILGMVAGGYTMAGIIEA
                     YPELTNNDVTAALQYASQVIDEEKVIPRA"
     gene            16761..17435
                     /locus_tag="PK224_05080"
     CDS             16761..17435
                     /locus_tag="PK224_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15138.1"
                     /translation="MNEARARGRAKSTIRKKCLTIGADHLVTLTYRANVEDRYRVLHD
                     LERLRRALSRSGCSMPYVAVLERQQRGALHPHLAVKGFQDVRLLRRCWYKIVGNGQGQ
                     VNVRGPRPGSSPVKLARYLSKYISKSFDNMPREFEEHRYFCSLGVKVPTEKHEFVLDR
                     RAKDVERKMYSIIRQEALRRVGVCCRLTEWMGGSGTYGWMSGFEDRSIQWVTGNSEAV
                     PITPSP"
     gene            17870..18985
                     /locus_tag="PK224_05085"
     CDS             17870..18985
                     /locus_tag="PK224_05085"
                     /inference="COORDINATES: protein motif:HMM:NF037056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15139.1"
                     /translation="MSDPFRHLQSVIDDLNLSLSIYDSFTSQILKSTSAIAAIQDSLA
                     AQRAIDRDLMASAASSLSAVRLPDFTSNFRDLVSANKALTNIVSQSVITDSALSKVFA
                     EHNALRDSLASIATLPSATDLLSSLDTTRLLHTSLSSQYRLLDLETHSFGSLIDASNV
                     LSNDLAATFSKLTRSYRDVIEAIPVIPQDFVPFIATYSPMEYSLELDVLEGISVERDE
                     EAEVEPLPSVDDELASFDGRLLTLLKGARESLNSDNPDKARHVTTSVRELFTHILHGL
                     APDAEITKWSTDAGHYHDNRPTRRARLLYICRKFSCDPLTKFVEDDVRAALALVSSLN
                     AGTHVVQSKLTQFQLQAIVYRMESLALFLLKVSRGGD"
     gene            19274..20533
                     /locus_tag="PK224_05090"
     CDS             19274..20533
                     /locus_tag="PK224_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HRB15140.1"
                     /translation="MSAHDPRRVVEDLRDHLAAHDRRLAFLLGAGTSSAVNVAPPPTP
                     GAKPEHKPIIPGVIDLTTICATAVSKIGNPYSAAWESMIKQCEKGGRKANVENVLSQV
                     RLKMDAIGEGETLVGLNRLQLIEVEKTICSTIADVVSPADSIISQRMPHHDFADWAKK
                     VSRTAPLEIFTTNYDVLLERALEASGVPVFDGFVGTLHPFFYPECLEDEDLLPKPKWI
                     RLWKLHGSVNWLLEAVESVGATRKRIIRGQPTKSGELILPSHRKYDESRKQPYMAYMD
                     RLLRILSVEHALLITCGYSFGDEHINAILYGALDNRNTANIISLQFGDLKETDDLVKA
                     AMHRPNLTVIGPNGAVISGIWGTWQLTEPVDAKTSSFMDAAFDSNALPEDEGSPATAS
                     ADLKGTMRLGDFNWLCQFLKAMGPGIQ"
     gene            20530..22320
                     /locus_tag="PK224_05095"
     CDS             20530..22320
                     /locus_tag="PK224_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15141.1"
                     /translation="MKQGIPSLIGHVDSVKGGVVTIRLRDDIPTFMMVDGRSYRVGQV
                     GAFLRIPLGYTQLYAVCTLVGAAAAPKSDEPSILPGHRWLSATLFGEAIGGVFERGVS
                     QYPTIEDEVHLVTLQDMHVIYGATKEERAITIGTIAATSGIPGSLDLGRLVSRHSAVV
                     GSSGSGKSNLVAVLVEAIATQGFPSARVLIIDPHGEYASAVGQYGKVFKIKPNANKGE
                     LPLHVPYWALPFDELQAIALGSMQPSSETAVRDEVTARKKSSARHLASAPPEAVITAD
                     SPIPFSIKKLWFDLDDFERQTFQDNTRTTPCNKTQVGDPENLVPNQYPAPNPGSQAPY
                     AHPRPRGIAKQLELMRSRIQDSRFAFLFHPGDELSPNLDGETTGDLHRLVQSWVGHDK
                     PLTVLDVSGLPSEVLSTIVGTLVRIVYDLLYWALDLPTSGRNQPLLIVLEEAHIFLPQ
                     GGDSAAHRMISKIAKEGRKYGVGLCVVTQRPVEIESTVLSQCGTMIALRLTNSADRAK
                     VEAAMPDDLGALSGMLPAMRTGEGIVLGEAMPIPSRIQFYKARKRPRGDDPEMPQAWR
                     QPRPDVVHYESALNNWRHQADMTGEETNDA"
     gene            22313..22525
                     /locus_tag="PK224_05100"
     CDS             22313..22525
                     /locus_tag="PK224_05100"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HRB15142.1"
                     /translation="MPEMIYVDSSNIEAVGYDAGSQELHVQFLGGGHYVYHQVPRYIF
                     DDFLSASSKGSFLNREIRNVYQFTKQ"
     gene            22614..24446
                     /locus_tag="PK224_05105"
     CDS             22614..24446
                     /locus_tag="PK224_05105"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HRB15143.1"
                     /translation="MFLTKNDFLVARTCPTKLYYKKFGYASILDDDPYREFLADGGYM
                     VEAMAKLLFPDGREVGQWEEPMRAFQETRESLAGGDTTLFEATVVHENLLTRVDILRR
                     QGAALHVIEVKSSSFNSETDGANPFRGKKGGIASEWREYLEDIAFQTVVLQRAFPESK
                     VVPFLCIVDKAKSATENATFDDFRVRRSSGAAGLAKTSVEYLGNADSLRKQHVLAILD
                     VTSEVGEIIADVAAAAEEFAKTLTSSPIQKIGPTIGVKCKKCEFRTERGDEQTNGFHE
                     CWGPLAEVQPHVLDLFRVDALGGKNRDVVAEMVAKNTALLTEVPNDAMQGAYAARQSF
                     QIEYTKNNREFISDKLPAILATHTYPLYFIDFECSRIAVPYHAGMRPYEISAFQWSCN
                     TISHPGGEVKHAGWLNADEVFPSFEFARTLKSQIGEEGTVYIWSNYEITVLREIRQQM
                     DSYGLNEPELGAWLDRVTTKGNPRVVDLLNLAKDHYFHPLMGGSLSIKYALRAAWTSN
                     NKLRANPVFAKYVKTDEHGRLLDPYLSLPPLPIGEKEEVVQEGTGAMRVYQEMMFGFV
                     KEDATRRESYRKLLLQYCELDTLAMVFLWTHWLTLSGQKEITPS"
     gene            24850..25584
                     /locus_tag="PK224_05110"
     CDS             24850..25584
                     /locus_tag="PK224_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HRB15144.1"
                     /translation="MATLADLIPDVSAVLALEPEELAGVALELLTSLGPHEPSRLHPS
                     SFTNSQTVGKYPDGQKHEIELALMEGWHWLVQEGLIAPLPGDTFGWHFITRRGKKIKN
                     REGLAVYRNAVILPRRLLHPMIVRACWSAFLRGDYDTSVFQAFKEIEVAIREAGKFKA
                     DDYGVDLARKAFHESSGPLTDQSKPVSERLALSHLVAGALGSYKNPHSHRKVELTAEE
                     AVEMIILSSHLLKIVDSRANHQTSAP"
     gene            25685..26749
                     /locus_tag="PK224_05115"
     CDS             25685..26749
                     /locus_tag="PK224_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15145.1"
                     /translation="MRLEGAKETDVRAEIAEPLLAALGYRRGTPNDISREVSLTYARQ
                     SLGRKKPSDPPLRGRADFVLAVLGAGRWVLETKPPHEPIDVDAIEQAITYARHPDISA
                     AYAVILNGVRLTVHHTSQTSAEKPLVDLTISDVASLAKQLAGLLSPAAVRRDCTPPIV
                     DLGSPLAEGLRSRVSIQGGQIRHGKSTWSVNIPLPDVEVQRLDEMCRRLGGLRIAITG
                     GTVYRDSESRIRAKLQWSMPHDELLRFVLDKKLMDIEYIALADEISTDPDAPSVFDVI
                     GDVEVSEGETLFNIATWETTTAGASIRMRYTGSAVGFFEKNVFHGVFSAHYYSEAPLL
                     PGFRMAMETGGVFEVVVDEK"
     gene            26895..27359
                     /locus_tag="PK224_05120"
     CDS             26895..27359
                     /locus_tag="PK224_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15146.1"
                     /translation="MEHNFEYLKAHILPLSRAKGFDVARTEWRLVAVEISEEIDNCPC
                     GQEIKEHCYIKNSVTGHETYVGNVCINRFIGIDTGNLFGGLRRIAENDTTNANADVIE
                     YANERGFLYDKEYEFLMSTRLKRTLSDKQKAWKRKINRRILDKIVVQRRTSR"
     gene            28011..28310
                     /locus_tag="PK224_05125"
     CDS             28011..28310
                     /locus_tag="PK224_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15147.1"
                     /translation="MSPKPKNTPHRVLLSLPTDLYNTISRVSAAMEKPRAEIIRDLLS
                     EQQPILEHMATMLEQAKAGKLDQAMKGWKKMTGEALKGLGVLMTPPDIRKAKPKA"
     gene            28368..28589
                     /locus_tag="PK224_05130"
     CDS             28368..28589
                     /locus_tag="PK224_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB15148.1"
                     /translation="MPEQVLKRLYSVREAARYLGVSPWSVRNLQKLRTLPCVRQGRRV
                     LFDIHDLERHIENHKTEVHDGHDLSPQET"
     gene            28555..29838
                     /locus_tag="PK224_05135"
     CDS             28555..29838
                     /locus_tag="PK224_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB15149.1"
                     /translation="MMGMIYRRKKRDPATGKLEEQGPYWMKYYVEGRPIQESTKVFEK
                     EQAKKILKVKEGEVAAGLYRGPSMDRIRFEDLAALVQQDYQINRRKTVRRITEYQKHL
                     EPYFRKARVSSMTTERINAYIIKRQEQGAANGTINREIGFLKRMFRLGYQQTPQLVAR
                     VPHIPQLKEHNIRSGFFEHEDFLALRGALPDYAQVPATLAYYSGMRMGEVYSLQWRQI
                     NWTEGKLFLKAQDTKTNTPRVLYLTGDLYRVLLAWKTRCDKKWPSCEWICHRGGIRLQ
                     SFKRSWWEACDAVGLGRMVKDEKKGRLVWDGKRPHDFRRTAVRNMIRAGVPEKIAMAI
                     SGHKTRSVFDRYNIVNERDLEQAARSLTTYFEKQTVTLAVTLAELRGESSGVANRQQV
                     DSSRIFVEPASGIEPPTCGLRRRRKKRGSRSKAVL"
     gene            30509..31450
                     /locus_tag="PK224_05140"
     CDS             30509..31450
                     /locus_tag="PK224_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB15150.1"
                     /translation="MESDRPLITSDLAPALIAAFLQERAAAIADTTVATYARKLTTFH
                     EWWRDHEPSLPVNETLATRYARWLATPSTRSKASQGLHLSAVRQWACFLLTAGHLSIN
                     PFAAVSGPPRARWLRHRLLTRTDLQALLKQFAVTTLVGQRDYLLAALMIRTGARESEL
                     ATANIGDLTEYGEEGSLLLLHSKGKAKQEPVVVRPDLSTKLWTYLRQRYPDGRFPSQD
                     PLFTNHHPSSRGERMTTRGMRKQLKAAFTEAGLAQPHVTALSLRLSGGALAYRKGASP
                     SELKKTMRHESIQTTQVLIDQVNRIRFGAERYLDDIQ"
     gene            complement(31774..32715)
                     /locus_tag="PK224_05145"
     CDS             complement(31774..32715)
                     /locus_tag="PK224_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15151.1"
                     /translation="MVRQKEKALASNPHILVAARLLTALTMLACTIDPSHAEMCEPDL
                     VNALKTRIHNYDHLKESRSLSTHYCTASQSDWRLGLSMIVDGIPANSTVNRQTMENAC
                     QGHDEQYLLDHHQEFETSYISDAALELCKGGLTFKAQRKSEKSISVAIGFIPTETPEF
                     AVFEAFEFSADAVNCVDLPQKGTQIFNKSINFTCKITNIHNDIDLKLKTEKHGSRVLS
                     IPRVPVSETVKPKWEFFVKGGNAGNKFQCTNEFGTVGGDIPCEVSNSPCENDRDGTKR
                     KLYCGAAYYREYKVIDGNTAVINDTQHGRSVRELPTR"
     gene            complement(32921..33439)
                     /locus_tag="PK224_05150"
     CDS             complement(32921..33439)
                     /locus_tag="PK224_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15152.1"
                     /translation="MLYHASSIRIEVGARWLLAHPISQVKVAGVIMWGFSAEIAYLHG
                     LRITRGWVCGRRTTLVEGERSYHAAGWDGRRAVREAITAWRRQGKEEREALQIQASLS
                     RIWVSAADSSDAGNCHSGTDFVSRKLSVRLGATGADLGAVRADYLLQYRNDEYTWRAV
                     RVAARRIVPVIE"
     gene            33890..34132
                     /locus_tag="PK224_05155"
     CDS             33890..34132
                     /locus_tag="PK224_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15153.1"
                     /translation="MTRDTAIGIVIHSLVTGFLILAAMQGAASANGEVWVLATYLGAS
                     IGATLYPNLTTRVSVMAGSVMAAFLWEFVFNHVVGK"
     gene            complement(34146..36611)
                     /locus_tag="PK224_05160"
     CDS             complement(34146..36611)
                     /locus_tag="PK224_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB15154.1"
                     /translation="MPQADGKKIRQLREQRGWSQDKLVDEVAKLREKDGSIEAPIVKR
                     TIQRLEMGCRAYPHTLYVVAKALGVHVHEITLGTADPVQVQSVAAPMAAVQRQHNIPP
                     PKDEFIGRTGEQSELRHLIKTHRLVTIAGPPGVGKTRIAMEIARSVTPDFHLVSYVPL
                     PLHEIALEQRIAYALHIDGQVQRDLRSLIRDTLKIGKQLLVLDNCEAVLEQCCKFVDE
                     LLSGCSDLCIVVTTRIDLGIKWEHIYRMPPLEVPNSDVGQSLTQLASIDSVKLLLARA
                     QAHRKFCLTTANARVVSDLCAKLEGIPLAIELAAAQLDVMSIDEICERFEDRLDLWND
                     EYVNEEETRGKSLRQSIEISCDLIRRHPNGKKIQRIFRRLSIFRGCTKSAVLSVCAEP
                     DDCEEDVVEILKALLRASLLQATDVAGERRFYYLDAIREFAADELRRAGETEAIAEKH
                     ARWFADHAEHAAPELLKTNQSVWLQRLGADADNFYSAISFSVSHHHAELALRITGALW
                     RAMEIKGFYRQASDKLRMVLRMPEAETFPLLRAKALSGLSTLAYRECDLDTADTASRQ
                     SLHLAKQTNDQLSIATALTDMGNVASMRGNYKEALRLFSESLKLERLTGHQRGIAVAL
                     CNSGLVARHLGKLDDARERLEESLQRFVSEGNMREAAFPLNILGEVACFQRYYRKGLH
                     FGERSYQIREDLGDRKGMAESLRTISRARVGMKEFDVAHTLLGKSGALVIQIGDQRGM
                     AECLEYFARLKVAENNFENAVILYALADHIRSKIHSPLGPADSAERTQILKKAQRTVT
                     SAQYREAWELGQSLEFTQALNFT"
     gene            37980..38177
                     /locus_tag="PK224_05165"
     CDS             37980..38177
                     /locus_tag="PK224_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15155.1"
                     /translation="MKGIGGVLLVVGGFVLYNSFQATQTIAFRLANLLGNEAAIQGVQ
                     LDMGVGFIMALIGGGLLTRDS"
     gene            38430..39731
                     /locus_tag="PK224_05170"
     CDS             38430..39731
                     /locus_tag="PK224_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB15156.1"
                     /translation="MKHSWLLLLQLVLAGLISGCAAGNGNSTHEIGSGELVTNLATLR
                     PLADQGNRDAQFNLGKAYHIGLGVPKDYEKALLWYRKAADQGEANAQTNLGMMYANGQ
                     GVSRDCPQAALWLRKAADQSNALALTALGFLHEIGNGVPQDYRLAAQLYRKAADLGYD
                     NAQTNLGVLYHNGRGVTKDYREAANWFRKAADQGEANAQSNLAFLYDLGNRGEPQDYG
                     KAAFWYRKAADQGHPNAQTNLAFLYETGHGVPQDYRQAEMWYRRAANQGYANAQANLA
                     SLHAQGLGLPQDLKLAFDWARKAAEQGHPLAQGQLGYMYHMGLGVTQDYQRAIYWYRK
                     AAAQGDPASQHNLKMLPPEILRKYDTTIGMEELTKTVVAIAATVAFVSIFDFISRGSG
                     TGTTRSNDGLRNTPGLDLVLDPYDGRHKTRYDLQTDKMNRD"
     gene            39909..41516
                     /locus_tag="PK224_05175"
     CDS             39909..41516
                     /locus_tag="PK224_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15157.1"
                     /translation="MSNWVTRVTTVLTLSMLLTGCASFVVKVRDDVAGLNRLKVRRAQ
                     EASLAVIAKRELGALKAQQPLKSGELLPPLFAQAQLQDAWKGSACSNQMGIHMNLTTV
                     GRGPMGEGASSTLAKVRGAFTAADQSKQRTARVIETGLEGTFDRTTGFLSLHAIPKST
                     ELTPDELEAELAIRAHVENQIFAIDQQLNSNPSASEKYKLMDEREKLQSDEKNRLKKF
                     TELAVAKAAAAKAELVPFIVELARDNDGKGWRGTIDGTAFQDCEAALVSDHGLNTEKL
                     PPITSQMILKKARSQGDLNSYWLNLAARDAREQDFFFLAQLYERHGVDSPENYVRAFD
                     YYKTINDKKEDARVQSALSRMYANGLGTPLNIPEAARLNALATETHKKALAVCKSPET
                     IALISEMAERARRKGKLFELIASWATGMEIDTLTTRVIKRMVGNVISIDQPFSCEIWA
                     RRIDPRINASLTPDYYRGYDQYGNYYEEDNGFEKGLKDAVGAAVQKLAQLTPVFTRVT
                     VEPQGQQRYQLIWSDGLHRESQMLDLN"
     gene            42828..43499
                     /locus_tag="PK224_05180"
     CDS             42828..43499
                     /locus_tag="PK224_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15158.1"
                     /translation="MSYKSLHLMMILLLVLGGCHPMVSTQPLSDPMTEEPDKSLYGHW
                     VSITDSGTQYHVYVGESGKNAGDVSKPFMECLLFSWKSDGSTKKINPMYSGYLTVSRI
                     GSSSYINVYATPDMEGKRIEAHTSSDNWRYETWLRNPDKLVGIFRYAVQENRVTFWEA
                     SNSKPLQQNLLERAELIAVPGNKLFSDIKPPDTPVTFDSLFRYLRKNGGETLFGSPTT
                     FTKVP"
     gene            complement(43652..43903)
                     /locus_tag="PK224_05185"
     CDS             complement(43652..43903)
                     /locus_tag="PK224_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB15159.1"
                     /translation="MTRCGLREAARPGEAAPSGSRRHRQRRVTPHSLRHTAATEAART
                     ESPFKVQAMLDHKDIRTTQKYFHSIDRLEEGAEYAITRY"
     gene            44691..46112
                     /locus_tag="PK224_05190"
     CDS             44691..46112
                     /locus_tag="PK224_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15160.1"
                     /translation="MRDLVIILPGITGSVLAYSPGENTPVDVWAPSGEALWQGLTSLG
                     DSVQRLEVPTHNPRHAPPETGFIPTRVVKDFHGVFGLGKIDGYTALTNLISDHFIVTP
                     GRPDNEVPANFFEFPYDWRLSNRHSATRLRDFVNTRLTRWRSGAPDAKIILLAHSMGG
                     LLARYYLEVLDGWRDCRALVTFGTPYRGAVNAINFIANGYKASFLDLTAVLRSCPAVY
                     ELLPIYRAVSENGTWRRVAEGTLPNAVNDYVTAASEFHHSIKQAVDNHLNDAEYLKNR
                     YRIFPFVGVSQPTLQSAILDGGTVRASTGCPDWIDNSLEGGDGTVPRVSATPIELSDD
                     YRETFFAERHASLQNNGYALDDVRERIKQLQSSGTREIMSEWRAGTRPAISLGLDDLY
                     LPGEPVLLYASVIGGRIPGLVARIESSVAETTERQFVATDSGWRLRLEGLSPGLYRVR
                     VRSTMGGDTQPPPVRDVFEIAGA"
     gene            46130..49822
                     /locus_tag="PK224_05195"
     CDS             46130..49822
                     /locus_tag="PK224_05195"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HRB15161.1"
                     /translation="MPWAAWAKINDKGLAQYVLPQIANRVRLSITRDDALTKPGGRRD
                     VTEAIFNALCTFDIRYARPLYNPIQEQQTIRAPETILDGSGDGTCLDLALLFAGVALG
                     NELLPLVVVLDGHAVVAVSMEFGRRDADHLRRPTDDGEWASTGILSHVTTLRALTDQN
                     RYLIVECTGFAKRDAIPADVPEGQGRVDGHLSFARAIKAGREQLERADRPLQFAVDVA
                     YLQDIGKQSTLDPVGRSLERVKPQLKRRLARIFETHQLFGGRSEELARLDAFLAGPSG
                     YMVITGGSGCGKTSLIANWIRQLETRSTHVAYHFISLQHDTAGHDETLLSLVQQLSYW
                     NSLPTLATSGAEVESSFIELITESTAPVVVVIDALDEAKGWQVGRQLFPARLAPNIHV
                     VLSARVVAGKDWITHLDLQRPEHIELGPLSQAEVEEVLRAAKVPAWVLDKNALTLLTD
                     KIAGDPFYLRVLVEDLLRGDIATPLALAKQPRGINEYLRQWWDEIRPTASAQAVQDLL
                     GYLLVARGPISASELKEVSPDDALSGFSFDAALENIRRFVVGDTNRGFSLSHWRFQDY
                     LTQEVLTDADQRPYRNRLEDWCDQWRTNKGPYALSYAVARRLDVLAAAPQSKRYDEIG
                     KVVTLICDEEYQKRRVEATDALASLKLDVPRVIQEIARSADVSVIPQLARVAMEHEHI
                     EHTWLLPESMLAPASQGYVEEAERRLALFQAEDHWRDAARLLIAWLAAPYDAGAARRA
                     LRASGEKWTGVPPLPMLRDRVLATIGAGDPPILTLPYYPNVLPEPRSEGEMQQIVAEL
                     GADQSATMRISGVEPVRVPGRQSLRARGTGKETPTYIAEGDAPHLVAFAIPATNREAG
                     TKLIRQYIGIHAANPYADYRNRSLWGILGAVLCHPETDWARDTTKLLVTGAFAPSPIR
                     FRESFRIAVLCRLDQAGVADGRAQFDRIVSETRAGAARIRETRWESDSWGNYSRRFSA
                     LAEAAAVALKDRTLAEAFLNEAAMLPVGFAGYQAPTSLTLAEANKIIRPNDTVSRSAA
                     LTQARNSAHNVQDPGFCARTTARVNAMREVWWAQPLENLTGILDEFIRDPFERRFAPI
                     HRIAEDFEARPKTEERIALDELKNARTLTDLAQNVFHLPVSAFERLNPGVPQEIAAVD
                     QIAVPDPKFAPLLAARFSAELVSRTDLSDDDRVSYLARLVPIALPNATTLDVVLARLT
                     LAMTTAAPLMLSELLAVAPALEDYPK"
     gene            complement(50226..51590)
                     /locus_tag="PK224_05200"
     CDS             complement(50226..51590)
                     /locus_tag="PK224_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4419 domain-containing protein"
                     /protein_id="HRB15162.1"
                     /translation="MDAVYVAFSQHRPLVLTPDAVWMTLAQGFAHHIMNHAEALRSSL
                     VRHKGKMILYAETIDPHASQHWAGVVQQWSDGIRQHVQDDLYQLMLCDFSTTSPVIRT
                     ASQVVMMEAFQQYFDYELKFICGIPSITLTGSVADWVRIRERVDVMATFQLDWWTDRL
                     KPICDGFVATAQGNPSQRFWKHIYSPKEIYGGDLITGWLADLFPYLIQSVTKAPTVRN
                     PILDIPRTQLTVQDGLKAGMIPTGLSRAPFTFKSASIDQSIELEILAGFLGVRQHVDT
                     GRLEPEIGWAVAEPELFTQLLNTLTRRGARDAISKPLVIAPQDCRLSNMLRVGIPKEL
                     VQMLDRFAEGHVFYEQTPHPWTLRPATEITGRAIGAADSSETVHFMDLADGRAVAYTT
                     VRGKDRARRAGRVLVGRLEGAAIQADSARVIAKGMTEFLDRVVREEGRYYFDAPSFES
                     EPLH"
     gene            52268..53584
                     /locus_tag="PK224_05205"
     CDS             52268..53584
                     /locus_tag="PK224_05205"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB15163.1"
                     /translation="MHTNISLLLTLLDLAPQLQCDLGTRWPEVCKSLLTLAGRLANEG
                     DTLSIRRDLDSLLDELFAILPETSSATLRSKLQDLHREAADQPSRLRAFGEQDRLTGD
                     REPESNDAIIVPVFYGTDRKRSDTPLSPYCGRWGEQDFGVAEVSVPISTKFRAIGELS
                     SPKWWRLEFNPDTKKHVVLLNVRQLAREIFIAELRDSLATADRRDALIFVHGYNVSFD
                     DAARRAAQLAVDLKFPGRTLLYSWSSSGLPQNYIMDEETIEWSTPHFEEFLQLAVTEV
                     GADSIHLLAHSMGNRALVDALSRFNAGALPPGSAHLQQVIFAAPDVNAGVFRQHAARF
                     QHHAKRFTLYASSTDLALKVSKLIHGHPRAGDVGDTPLIVDGIDTIDATTVDTSLLGL
                     KHSYFGDKRSILNDIFGLVQNGHEPKKRFDLTAAISSAGAYWLYRP"
     gene            complement(53594..56203)
                     /locus_tag="PK224_05210"
     CDS             complement(53594..56203)
                     /locus_tag="PK224_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRB15164.1"
                     /translation="MIGYANCLGMNPGVESDPLVYAFEMIERLVTRHRGAGPSDTLAH
                     ALGCYSQLVSTAPVGDQDANIEHAIALQMEQIEVLGSLSSSDASRFGRAQHNLAGLYL
                     KRRVGLRTQNVDRAVTALQKALVVRTRESDPAGRARTLRGLALALREWSGAESQAHAN
                     NLAMAFQQEADEIVQEEPGAAIRTGEWGHFSGQQSALYADLNEIVRLPVRSALASLDA
                     AILKHRSTLSGIDIKRMPNEWANWRAGLGYLLAKRYHLSRERRDIEQSCECFVSALTA
                     IDPSSNPRLYRDISRTMGDMCHQIALWEGSLRANGDALALSEQLLDDAVTSEERQKKL
                     ADMRGIALFACYAAVRLGQYEEAARLAEAGRARGSVDLMVASEMLTASLSPESRDEVR
                     NALQRVRTLEEDIRGLRAKNPERVMADIAGRLADHFGVDAGAIKVRITQGSAQVQEFI
                     NLELQKVADLRKARVWLRETTARVRSENLVSLPDRLQSSDLAALAKRFGQPIVYVLAT
                     AWGGAALIVHSSGAMTGILLEDITSDVVTGLLEDGMLDGAMGHDHCALAARLPTALSV
                     LRDTVVEPIADRLREYGYAQATLIPLGRLGLLPLHAAATGRGPAFAYAPSARALAHSL
                     SLTANRPARGYVLCGVADPTMSLPFARAEVQGAVNEPGRWTRTTVFVGKCATKRAVAE
                     AAHVSTHLHFACHGLFRPSEPEASGLVLADGILTLGELLRGHIHFSAAELMVLSACQT
                     ANPEFREVPDEVLGLPLGLMLAGVPRIACTMWRVDDRVAALFSQQLYQELFVNRRSGA
                     AAVDAAQSWLREATVGTLKGTVSSLRRALGNHDDESEMMLSNLWRDLLSRDPTDTPFA
                     SPEHWAAFAYVGI"
     gene            complement(56598..57497)
                     /locus_tag="PK224_05215"
     CDS             complement(56598..57497)
                     /locus_tag="PK224_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15165.1"
                     /translation="MYLTKEEKERIGALFDAIRKYAILDQEPLEVLTDELDRTMQYMD
                     ALSAERSQQQAILDEQSLDATKKLTEALKAELEAKAEAEARARGAEAAKTLSEEAAKK
                     AEEARKKAEEAAKKAEEARKQKEEAKKKAEAAAKASAATSAPSQGRTVDDGLGGTSGC
                     GNRSNAARRVQALYQCTSGDSGGTTHTFSGAHAPTHGELSGNSNIMLVDPEQTTSPIS
                     TVLSCAVQAGDLQRISTNDPKCGAMRCFQDRPCPCNKIQLEENRTQVRPPGGIAPDCI
                     GEGPCGVSPMVPGGSTPVDPRRR"
     gene            complement(58279..59244)
                     /locus_tag="PK224_05220"
     CDS             complement(58279..59244)
                     /locus_tag="PK224_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRB15166.1"
                     /translation="MRDALGRVPVMIVTGFLGSGKTTLLNHLLTDGKLGRVAALVNDF
                     GAINIDAALVGSMADEVVQLTNGCVCCTINGDLLAAAERMLTVEPPIDHLIIETTGLA
                     DPLPIGLTFLQTGLRSRTSLDAVITVVDSANFALDIFKSDAAMAQIVHGDVIVLNKTD
                     LVGEEHLSLLERRMSLIKPRTRVLRASYGRIPHAVMVGGFGESSLASVSDPEPINSPH
                     LAKDDFAAHVLHFEGFLSAQLFQAWLDHAVPVGIFRAKGIIALDSPERRFIFQLCGSR
                     ASFEPYAGPITDCQLVFIGQGIDGPALLAAVQSCVVRTKILTNTS"
     gene            complement(59244..59663)
                     /locus_tag="PK224_05225"
     CDS             complement(59244..59663)
                     /locus_tag="PK224_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15167.1"
                     /translation="MAAMTVAPGLRVAPTLNTFPLVELTAENEIVVPSARRLLAGMLA
                     PMHPLSHGGAPAPPPFLRREQVRVIDPVVAIRSLTDAGRAIGKVPLAELFRSVKRLHP
                     NAETHGAPGDGEAHVLRIANYDANGQLVGGYTMVRTS"
     gene            complement(60392..62722)
                     /locus_tag="PK224_05230"
     CDS             complement(60392..62722)
                     /locus_tag="PK224_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRB15168.1"
                     /translation="MPGWYIHINVARKGIDALSSNPTAAPIFAANGPDAASLTQIAHD
                     EPAYAALGSIGPDIFFLLPDFKPPVGSMLWKLAGEIRDIYTWWDDNFLGPFESAIGPI
                     GNNASDEINALTGGLKDTLESIFGQAFSFLKDSILRLILQQYDFFGLLSSGVPAGYDE
                     QTFFWSDMLHYRETYRFGAVLWQRAEQEPDPVLRRRFQAFALGWMSHLATDVTGHAFV
                     NQKAGGPYRLHWQRHHLVENHMDALVYNTDHGAQPIYNQMANSALHLWLAFNPDGSSR
                     VDFFQAEPNPPYPDGDHSADITGRHAVWDFDSDLPDELAQFLSDTLKAVYTPSLSAPS
                     TGLVACCPTIVSTLDGRVPLATQGFAEKEDIVGAYWWLYRYVKWTTTDYYKIRRPEQP
                     GVFVIPSFPLPPGSGDSDPGPGVGDDSSMWNDVFDFLLSLFAWATYLVEVAVWPAAVI
                     AGIIAGTATYPLRVALYEYLELPLYNLWLAVHTYLALTGYVMPMPGEINSGLNTLGTS
                     VADDWAATVVALGDFNGGVTPVTVSSDPSGNPKTNYPKDVLLDPPNAITAMFSEIFKI
                     GSPPVNGEFPSEFTRPWRYPEKDNQGNTVNIEHRQNIAGPFIAGMDATALFGSAPGSP
                     DARTQFEKARNEEETIRLALNLLPLGQHLGDPTDYAAYVIAKLTRNDLDPATVTNFNL
                     DSDRGYGYLVWDWLRDKRRLSVPDAFRGNGDPSTGGTPTNVSQHVYPSPVQPGYGWNI
                     ADEFDNGGQPQPQGFNPNDPQASVRIRYINRERKFI"
     gene            64793..71947
                     /locus_tag="PK224_05235"
     CDS             64793..71947
                     /locus_tag="PK224_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15169.1"
                     /translation="MPFELSLVDLVSPYVLQGDTFGKWHAVLSVFRVSQHEIAHDENG
                     ITIRGTVAFEGNLSIDPSSMALTWANAENHPEKDASRRDPWIDVRDAKLDFQLVVPRV
                     ASQKVSTAVAAIGGNGAFTNSAQVLTAYDTNPNDAPPSDYPTTGFILDLLLTSVVLRP
                     PFLRGARREANGQLVLDPQNEQVKFTLPRLKFQLAQGQANTDPLTATLLSAGASGLDD
                     PGDIAVAELIKMDPPYAFIGPSQVVGFGFRSGTLDLSDQSTPPDVLAQFGFDESWTGL
                     YLPEIRLFVAPNGARDFAVEAGVENLLIGIGTSSGVTGDFSLQVLDQGAGPLKLSARF
                     YDGDQRGYGLVRTSNTSGTVQLPTQSRIVVDVEGGRAPVTVTIAVDGGSAQNVREIDI
                     DLSTTPTRTIVINASDTSSPVRSATFTIQAGLKRPQGEVPGTSSLGPTPTARIITQST
                     VQGTATVQQPRLQIISETASTATVGLDVSSVRAPLTQWTVDGSARGMSATLVIDLSAG
                     GPDVNVVATLPGESGVTDFTGYFRFDHPPYSENNRVQTDEDTRAFALDPNNSRTTEAN
                     DEGTTSNWLGGSDIRTALLPVLQSLGPNTAVSIEGFASYEGPPPPTVDETKRAYNADL
                     ARRRALGFRAVIEDLIGNPANNLQGKHLTVTHAANMTNWTNQGFPAVETRRSWWKAVA
                     RWSAATIRGTTTSGTLHRDPRTSSNKPPKVQDPPNQQSAPPPRWFKQMGAKVRIVRNQ
                     FVACEITAKIDIQTAAEDRLQAGMPSGRSGTLGSGQALGANPADGIIDVRLVIQIDDA
                     TDTVTVIGYYGADPADVDGLYLWGTRPGQAATTNPGFGLNFFGTTIVFMPLISAGVGA
                     VSNDGALAELGMTAAMLAIPATIAGLAEAGGSPVKVLCERVIWYGGEVQFRKRPSGAE
                     AVILFDMEAALSMDVSIGGKTLLKIDRNAPLAVRYKAVGIRIGDDPDQPKFQFRPVFD
                     ASRGYTIDVSRPGAITVASPLDQILTILGARIARNNPLIFEIDLGFAVDLGVVTIERA
                     RVRLRFDPLGPPELTAFAAGIDIPGALRGRGYLEMNESEIKGQIDLTIVPVQVRIAAG
                     VGVANINENGRKATGVIVSLEVEFPVAIPLGNSGLGIYGFLGLFAMHYSRKEPPPSAM
                     APALAWLKDIAQGNPTDIAAWSPKIDTWAFGVGAVLGTMGSSVIFNLKGVVLLELPGP
                     RLLLMMRANLLAVLPKLKGTAEGTFLAVIDLDMGRGTLTIGLSVEFGIQPLLEIHIPV
                     EAFFNFHDSKDWHLYLGRFIEQIHAKILEVFDGSGYLMLSGKGFTAGDLADGLPVPVN
                     GFAISTGLHVSFVWGSKSVGLYAEVAAGVDAVLGFDPFRLTGVLYLRGTLHVFIIDLS
                     AWAKLTVDIGEKPDGSKVASISGQICGRVKFLFFSIEGCVDFALGQASIPPVAPPELF
                     QSLRLVSRSPALAVGTGVDKAIDGGIAEGIKGDVAPAPPPNPPPAAPLGQPQPDVAMN
                     QRRVPIDAIPLAMLAMPPVTGSTQYLFRGTAVNQAPGPGTPGAPPDGWIQKGDDVFQY
                     TLTSVELIGDLLDGSTPAVWWPQKAGDTPTQAQLGLLSWVPDPTPKAIERSHFLEETV
                     KETWGTVCFPAAAPTSVLFTFLHEPFGPSTYGWVLDGKPWPDPPDTIRSVPPTLTLKV
                     SERWRTASDEVDELAGVIPAVVEGAPVVCQHKPNQPDTPIHAIPGTVPIPVPTRALRF
                     DPIAAARGVKTPDAFTPQEMTLVDLARRANAGLEVSRSMMTTLVVTPTTAAATADRPC
                     TSRVLAAPVLDSKNAQPFGPPSRLIQLQKERSRRGFKPGPLDDAVVFYTGPVETATFY
                     LFVPRDLLAQKIVVVAATDGQDTVFQQLVLDPSMMSPPNTLPPRWIDASGPWFEEAAH
                     LAQHQSVLRPRGFVGVIVTIKGHQRADRIQIGLTQHDADWHRKTVERPFYVAGIEVLT
                     VAEFVRFDYDTKEQKKKRGVLEAALSDSSSGQALLKPNTAYGVKVTYDAKRGKRPPGQ
                     AVSDEVSALNQHQTFWFYTDDQPPRRLDPWLMCSTPEDGEHHYFGRLPVHIVFNSPDM
                     GRIYDAYGRRLQIRLRAASFRPLPSSPTAPHPLPLTPDFLKPVKAALLSPWEGVVQDQ
                     LIASCVPISVERARHSMVTMPIPLEPFTDYVLDIEMADKNAPNDAPGLVVWRTGFSTG
                     RFATAPAFAESFQLNRLLHRFSKPGQLQAIGTQPWAANPQGNQLDQAMMAAGLEPMGV
                     PEAPRIMVFWETAASDPQPAAVLVDSSEPMWRSRRIPKEAMDPNPSNAKRYEMTTTEW
                     LRLEEEAGGDAIVDKVIQAPGGQRALVTLKPNSRGKTLRLALRLVAQKEPYLDGPTAA
                     DQLFTVINAQLNRAPWEEED"
     gene            71947..75588
                     /locus_tag="PK224_05240"
     CDS             71947..75588
                     /locus_tag="PK224_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15170.1"
                     /translation="MAFIVDITKFQAEGALLNWPVVARRLGMLDATTGGIQLPSVLQL
                     RWTLNNELGLPTEPFIVWRRNKKLRGPKTIAAEVTTIGLLGNSQLLDFKASYSTVELN
                     VSGSGGVVYAFVGSPWLNSIVAIASISVGNNIVVTLAAPSMEGLIVSSGVVVNNIAGV
                     RSDDLTVADGWQKVEFVGLPVKEDDWAGQGIGRHGVDQGIFGAFTDAISAAKQRLERG
                     APPIGWAPAIESGVAAPPWSAPVPNQLLNDLNAMLLDDLRTVAPLPPATQAAKKISKP
                     MPPPENSSGDQMSGPGGTSQVSPLSSTYMAAAIDCFNCLALGFGTAYPLRLSTTGQAE
                     NPLDFDYMITAQYEKGLRGNGPAVAYAALVPSPGQAIAPPIPANMAQQMMGNLRPLTR
                     DGSWLASVRISWDRPVPISLFRPRSFAFVRSEVTPPSPAVLLMNKRSDGSPLPVAVNY
                     LVTPQDPEPNRLSAVEREIPIPNNPGTRTIKYAAAHQDIYGQWSSWVAINASVNQPDV
                     DEPRIVSAEFKYTSVPSPPAGICHANLVLEFLWDWRIRTPLTINFRGRLHAAAYHGAP
                     PPNITLPSGLQVKLEVAPINTFTLKFDLLAANGAPTSSWPGYDPLTHCVALSQAGDQQ
                     VPFGHAQGGETRRYRVTIPGLELSFGPTGHIGLALWAQGQESIAPQRNGNWSKEPSVI
                     STSDPRPPLIAPDIVTLSSLPDAAGESHAVLKWSGSPGADGYFIYETTESKLLKAVGE
                     SEPDPSKTLSQRLTKLRAIFDAHPAERRTEFTRRNSRLIKTTSSDITLPRGSTAIHLF
                     AVLGVSAGQVEASWPIASTALYAFAVPRVPKPGAPTIEVSSFSDMNVTPNVYRSKIRI
                     ETRKGPRVRRIDLHRVRVDDAARDLDTMGPPVLTLDAMTPGWQVDRINDALGEHITVA
                     KGKETPPGSWKRVWYRAVAWSADDPLRGTLAARSPASTVAWVVIPPSTPPDLSGITME
                     WPGTAPQDVMLKWTSMTPTKKTPLGPHTLAVRIKRIGAPLEEASLLTFDGPLSDLATI
                     QAPGGSAVWRIEATKPTQYRAIIRRADINDAIQISLRLTDPVGRSAESLATINPGHIL
                     PDPVLADVLLKKSLNPPGVMLSWSSDTPLEPPVYSVRVTVNQPPRLIGSFRIPQPPII
                     QQMSLATVPMDEPGPVPLGADPLRVRRMPGAGPKYTYYAFVRVSFTQIILRLSSPDGR
                     FAEHIQLPS"
     gene            75594..78962
                     /locus_tag="PK224_05245"
     CDS             75594..78962
                     /locus_tag="PK224_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15171.1"
                     /translation="MPPVTITTLTPTAIQQSLQSNGLAALGLTTFSLSPRWGDTTPIA
                     GDYDNNTLTLTITGLMRAPFLSIRENAFTDATSTLAADLPGRDTETTVTLAPGTGNAF
                     PAPTGGGRVLLTLSNSSGNTREVVECTARTGDVLTVVRGAHGTAKQHFSSGDRVTLRL
                     TPAQRSSEFSGVDGAPLNVACTVFRLHPQAYLRLQTLCTTRYAAPGQPLTLPLPATMV
                     VRGAAGFRASRWYRPDEPLDDVTGEISFHDNRGFIIDPIYVAGLFADLLTTLPGLVPP
                     AVTASANGPGGVQGISILTTGILVHFIDPHGNSPRIATPGVNLITTNAGTTVSGNLIP
                     LSEGDTIAANSATTPLRFGFATNGTMSTAPLSIPALTNGTIARRFYRVMVVDTSWYLL
                     GNRTAGTVLGVGPDDQRIPTDLLPVVRDQVTIDYLVDGPDTLAEATRILTRPSQSIVL
                     AVSPVIDQTLITATQRGTTAHWPGFPPPNVNAGFPNPPVRLSSTNVTAAFTAGNDVVV
                     TVAGGAVPDGATIRIYPQVFIEIASINGSEPSFVRGDGGANIVTGATPTNILLRNPFN
                     LGAVQPHPSPAILTMDIVVVPRNGRRRLTAAVAVPVAAGPATVPTDPFFGPVGANIMG
                     IMLPVTQGVAQSPLFGIPSAITPPAVPPTNLLELALSLAAEPAPRQAPRLPTMGRLET
                     VAVTGTTDAGAQPSGSLLWQAVLTGARWAPESRSAQHAEGNPGNPAGPDVHAPGVSVT
                     GALAYDLALHALKRAQSLIPRPLPGGGMSPGWVFSSAGDNFDEPSDIANTANTGMGVL
                     LETVAVGCETSWLASLSPPQPGFTLNQLVQNVASAMSLPTPPTNAITVENEPRIQRQI
                     RREFFAAKHGFRDAQWSLRRAFAEARELVYIESPQFARTARPSGSPQNFELDLVAALA
                     ARLAAHPTLRVIICTPRFSDFDGTFRSFHRQHYQARMEALGNLKAAAGDRVLLFHPVG
                     FPGRVAYIRTTSIIVDDVWCLVGATHFRRRGMTFDGSATIASFDRQIIDGYSTKVRNY
                     RRTLMAEKLNVPAPSPGQPLSGEWVRLGQPVSTFELVRDLLEQGGLGRIQGIWPGPSD
                     NSVLPAQPEVADPDGSNGATMLTTLAGMLNELGT"
     gene            79130..80533
                     /locus_tag="PK224_05250"
     CDS             79130..80533
                     /locus_tag="PK224_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15172.1"
                     /translation="MRTTIVRRNDFWIVTLAFYTLLSGCGPSPLPTAACPQSYIKPIS
                     LQVAAQSATSTVDLALSQSYMRKRIKDTIQTPDTDSQGHATSHGIDVNQITLLEKTVN
                     GQDLHLIAINMTPWLRGQNGAHVSSNNTYELFLKLNPYIVDQNTVPDAAQRKKVLCGT
                     NNTCTENEGVLLGFELHELKNKSQTTVVATGNGICGTVNLIDEKVLDGIWGIDGGLTN
                     ATPVVLPTASIAQMITGFAGVPVRVTGLALGSNTDLKIGMLMNAGAGHAFDSSTGFRP
                     AVDWTLDLDSSVIKDFISMTANAVAASNNPPATVSDIAVKFIDQPENAVDVVVNLTLP
                     ICSTAKVRVLSHVRFDVCTDMNSQPILQQCKADDQRLPNNWPTNACVIADHLFRWPYI
                     GNAIDPPCGLANSSTVLKFQVGPAEAFYATSMDVSDINFHIDGRSTVMDTVAAAQGIS
                     RVAKPHACDSSFSFHFP"
BASE COUNT        18816 a        22408 c        21418 g        18262 t
ORIGIN      
        1 gccaggcacc acttaaggaa tggcctaggc tgtccaacca accggagcca cctctctgcg
       61 ccaggtgaag atcattgatg caaatcgtat tgatcctgaa cgaaaccaat gccagctgtt
      121 ctgacctgcc atgagtattg tgcggacatg aatgagcggt gtcggacgac aacgacgggg
      181 aaccgatgcc cttaaagcct aagtatgtat tgtatgttga tgacactggc agtcgcgatc
      241 cagacaccca acctcttctc actcgaaaag accggatgga ttgttttggt cttggaggct
      301 tccacctgaa agaggaagat attctgatgt gcgacgcaaa catgtccagt tttgcatgaa
      361 atggaaaatt acctatcccc tgcattcgtc ctcaattcga ggtgggcgcg gagattttgc
      421 ctggctgaag aagccagaga cggccggact cttttttcaa tccctcgaag gcttcttgct
      481 ttccttacca atacttggcg tcgcgtgcgt gattcaccga cccgggtatg tggctcgcta
      541 tcaagatcgt tacaaggagg cactatggta tatgtgcaag acggcgttct ccattctgat
      601 cgagcgcgcg gcgaaatttg ccgatgagca agatcgattc ctggaggtgg tctttgaatc
      661 aggacaggac agtttttaaa atgctcggtg tttcaaatgg tttccccatt ttaactgtag
      721 aaacaacgac atgctgcttt ctagggaatc gataggttcg agaagtatta tgacttccgg
      781 atatttttag cacaaattta gcacacccat ttggggtgaa ccccctcggt tgcgacagtc
      841 ctgatgaccc tctggccttc ttcatcgatg agacagaatc cgaatatcca aaagtatgac
      901 cgagtctttc aggttgaatc gatccatcag tatgggtact gctcatttaa tgtctctagt
      961 tgaatcttac acctcaaaaa aaggccccaa aggacggaag ctgtcattct tgctagagga
     1021 tggtcatcat ttaaattgtc cttatgcagc gacaaaacat tctgcttgtt aaccccgtta
     1081 aagtgagtca aattcacttt gctcagattt tggtatcctg cccaccagat gctcttcgaa
     1141 aaattcagtt ccttggttag tgtcccggaa agaattttat cctgttcgtt taaagactcg
     1201 gctcctattg gctttccacg cataattcca taataggttg gcttgccggc caaacactca
     1261 aaaagaactg agaatagaag gataccttct gcccgttccc tccaaacaag gcctagcttc
     1321 gagtacgtat cgaacacatc ctcggccata taaacttccc atttttcgtc attgcctccc
     1381 tctcctagtt gattctgcag tatgtctcgc acatttcgcc aatactcttt gtaaatatcc
     1441 ttctgaagcc attccaaagc tttgaaaata tccagagcga cctctaattt atctggctgt
     1501 cgaagtaaaa cagtaagttc gtcagtcatg ttcagcctcc ggatatcatt cgaaaagtta
     1561 taacaagtct caatatattg agtgaggatg acctgtaatc gtcctggcac agagcttgat
     1621 gctgttgata gccagtctcc gaggccgaga tatgagatac accgaacatg ctccgaggta
     1681 gaagtttttg gttttctccc cgtgggggtc agaaatgcaa gtagatgctc tccaatccgg
     1741 ggttctggtt gaagattcag ccagtttaga tatctcgcaa tttgctcctc tccctcttgc
     1801 gcattgatct tattttcaat cactaatatt tgtccggatg gccagtagat tgttatatcc
     1861 agtcgaccaa acggttctac acattgttcc ctcatgacaa tcgcattaat ggaatcttga
     1921 ggaatagggt ggcaacctat aacagtctcc atgaatccac gtaaaaacat atcttcttga
     1981 tcatgtgatg cgcgggggtc taagagatga ccaaggaatg acgaatgata atcttcaagg
     2041 cctgccaagc ccatcacgtt gaaaatatta aagcgggacg ctgttcgctt ttgctccata
     2101 agccgctgct gtagaattgg cgaagtgtta gcaataagat catcggccct cttaataagg
     2161 caaatcgctt ctgtcttctg gactgattga cactcatcaa ttagcttctg ataaacatct
     2221 aaactagact gtgccatgac ttaaacgaga gactcccatt aatatacgga tcagaactca
     2281 tcccacccag tgtatactta ctgtacccaa ggatactttg ggctccctat cctttattac
     2341 tcggtatttg tgcctactct catcaggacg agatttcaaa gggatccgtt cacattctgc
     2401 gcacctaatc gctcaggtct ctgcaccacc ggcattggaa tgttggactc tacggaagaa
     2461 gtgctcgttt tttacctggt cgccatccaa ttcaagaggc gcgagggaaa tctccacctt
     2521 taagaatgca cagtcgatct cgcggttttt aacgtaaact ctttatgacc attcgtattc
     2581 cgatgcgtat atatcaacta aggctgccct tgcatcccaa accttagcct cggttacgtt
     2641 tcggggacga tgagaatgat accatcttga ctgccacaac tttgctagtg cgagctcgta
     2701 gccctcccac gttttaatct ttgaaacagg tatcccaaag agacgagata gtcgaaatac
     2761 ggattcgtta tttacagaga tgaacaagtc gggtcgagat agtacgagga agcgcgtggc
     2821 gactgcagac ttaattccct ttactctcac caaacgcaaa aataggctac gtgcgatggc
     2881 taatgctgat tgagtatttc ttgctctaat aagccttgga ataagtttgg taatgtcgcg
     2941 acgcaatctc aaatctctca ttaatgcagg aactgcgcgg atagctcctg cgatactccc
     3001 aaaatagcct gtattagggt acgtttcatt cccacctatg agatagcgaa aatcttcctt
     3061 tgtgagctgg tcaaatctct tctggattat gggtcgtata acatctaatg tctttaaata
     3121 tgactcgtct ttatccagca gactactctt tccgtccgca cgcgtagctt gtttctgtaa
     3181 gagccgataa tagtcctgcc aagtcagtgt cagaaggtcc ccaactgtgg atgccgaagc
     3241 tgagctctga ttctcccttg gactttgcga tttaagtatt ttttttaaaa tgccactttt
     3301 cggaggtttg tattgtttcc tgtatcgttt aagccaggcc gtagttacgg gagtagctgc
     3361 tcgccctagg ttacggaaat gctctatcag aggcagcgcc gatttgtgat ccagtttaaa
     3421 actgactgat acttctgcat taacagtgaa agccccgcat gttaggttcg ggctaccaat
     3481 gataatgctg tattgtgaac ctgtttggaa aagataaacc tttggatgga acaaaccgaa
     3541 atctgtcttt cgctcgtcaa catagacttt tccaagactc ataagcgctg caatagcatc
     3601 aggatgcgta atgttgaagg ctttcccgac gattgcttct atgggtatct tagcgttctt
     3661 taacatatcg acgtgaacgc ctagcgttgc ccatgccgct gcaatcttga gcgagtctgc
     3721 cttatttaag agagtcgcaa actcttgctg taagtcctct ggagtaaaaa gaaatttgcc
     3781 gttgaacggc ggcgaagtgc ttgccttcat agaagccatt tccgttttct tccgcagtgt
     3841 atccttagcg gttaacctca cttcacatcc ttaccactat tacactccgt atccagatcg
     3901 agattgatca cttgatattt tttcagcata tctgaaagtt cctctcgttg gatgagatta
     3961 actcggttcg cttcggcttg ttctcgggcg ccagatgtaa atcgctgatt ggtgacggcc
     4021 acctttgaaa aacgggttcc tgagaacctc gcctgataca gagctgcccc tgcgacaacc
     4081 tccttaatag catcccatcc aatctcggaa tgaagagacg tcttgcactg tagtaattgt
     4141 ccttccatcc caagctgggc tatcacgtct attccaccat caccacctcg tttcgatgtg
     4201 acattgctga tgaaaccgcg tttctcccac agcaatttgc aaaagacttc gaaggtatag
     4261 ccatccatcc tatcaacatc atccatggtc aacacccgct tctgaagcgt ctgaccatga
     4321 gtgccgtcac cgaccaattc tccaatacca atatctgccc ctactccgtt aagcataccg
     4381 actatcgctg attcctccag cgttgcgtcg gctaatttag ccttttgctt caacatatcg
     4441 tcgagtcgga cctcaaatgt aggaaatgca tccgtcacca ttgtggggca gtaaacgaaa
     4501 acattcttcg tttgccccat ccgatatgcc cgatcagtag cctgatcctc cttggctgga
     4561 ttccacgcgc gggtaaaatg gaagacatga ttcgcagcag tcacattcaa tcctgcgccc
     4621 gcagccaaag ttgaaagaat aatcacgttg aatccatcac tttgcgtaaa tcgatctatg
     4681 tatccttgcc gaccctcggt atcgccgttt atggtaaacg gcttcagtcc gaaggtctgt
     4741 cggatgaagt aggccaaggc aagttggatc tcgcgtatct cagtgaatat aattaccttg
     4801 tcaccattgg tcgagtcgcg aatttgtttt agggtgctca gaaaccaagc catcttgcgg
     4861 gaattcaaaa gatgcacatc gatgccacgc tcatcgacca agaacttttt tcccggaaca
     4921 caatatggct ctgcacaaac agccttcatt aggtggaacg cctcgaagga caaaagagat
     4981 cgcctacgcc catctcgttc ctcgtttgcc tcttgaagac gcttcagccc acctctgtaa
     5041 agtgccacct ggtgatctga catagcgatt tctaatcgca tgtcatcctt gagatcaggc
     5101 acaaactgga gcgcgtcatt tagctttgca tgcgcgaata atttgagagg aagttccgat
     5161 gcaatatgag ctttagttcg acgtaaggtc tgtggctcga ttgtttcttt tagtttttgg
     5221 agcgcggcca aaagctcggg ggtatcactt tcgatggggc gccggtatgt ctgcccgaac
     5281 tcttcgaggc ctttcagtaa gcctggctga acaaaatcaa ataaacacca taggtctgcg
     5341 agtgagttct cgaccggggt acctgtgcag gccacacgaa agtctgcgcg taacttctta
     5401 acggacagtg ttacctgcgt acccggtgtc ttaatgcgct gcgcttcatc acaaatgata
     5461 aacgcaaatg gctgcttcgc gaatgagaat tcataacccg tcaagacttc gtaagtcgta
     5521 atgacaatct ttgcgtttcc taaccaacct gggcacagaa gctcggttac gcctcgagtc
     5581 tgaagttgct cgtcaatcag tcccagtggt tgcttccgct tattaagttc gccattgtag
     5641 agcacaagcg attccgggaa acttggcgta aaaaatcttt cgatctcctt cttccaatta
     5701 tccaatagac tcttcggggc taagatcagg gaaggaggcg cctcgggatt tctctcatag
     5761 tgctgagcta ataggcagag caattgaaga gtctttccca accccatatc gtcagccaac
     5821 agggcgccgc gacagtcggc tggcgctcgg gatacaagat gttgaaacca tgcaacacca
     5881 aagcgttggt gtttcttaag ttccacgctt ggacgcaaac actccggcag cctcggctgc
     5941 tgatcttcat ccacactcaa taacgtcttg cgggcctccg cataatccac accgtaaaaa
     6001 ttcagcttta caagtaacct ttccttttca cgcttctctc tcgtcccctt caggctcttt
     6061 gagacgttca cagtctgctg ggctgccagc atcgacttaa aagcatcaac caatgctcgc
     6121 gcttgccctg tctcaatcgg ggtcggcagg gaatcatttg ttactgtcgg aactgcaccg
     6181 ctttctgctt ttttaacttc cttatcaaaa tcctcgaccc attcctttgt gagcggaatg
     6241 agcaatcttc cttcatgccc ggctaggctg actgcaacca ttggtgtgag atcatttggg
     6301 atccatccag gcttgggttc ctcttcgtcg gaagactttt gaaataccgg cacatagata
     6361 ggcttcgcaa tgccaatccc ttctatgcgt tcgctgtagt cagccaaggc atatacttca
     6421 tcgaagccta ttcttttgct gggctgttgc ttccagaggg aatagatttg ccgagcctct
     6481 tccaactgca ttgtggaatc gccgtcaatc gtcaaatcga attcgtccca cgcgaacagc
     6541 tgccgctcat ccgaaatcgc ctgcctcaat tttgcaacga aaacacccag ttcgcttgga
     6601 ccgccaaaat gcttcgttgt agttcggcca cagccatctt cgaatacctc actgattaca
     6661 aggtcaactc gttcaattcg tccattctcc gtccgaagac gtatgtcgaa aatggttgcc
     6721 aacgcaccaa cagcggcttt gtcttttagg aattcctctt ctcgaatgac cgattgagct
     6781 gtatcgccaa gataggccca gggattatgg ataagctttt cggcgcggga gcccgcaatt
     6841 cgccgggttg gcatctcgcg tttgatcgct cgaaggaccc gtttgacggg ctctgacaga
     6901 acaacgcgga ctcgtctacc atcagaaatc aaatcgtagt gatcttgaac ttcacgaaag
     6961 ccatcaaatg cactcagcca ttcttcaggc gcttcatcga acgtaggtgc gactgtgaga
     7021 acacgctccc ctagcacttc ttctttggta aggggtaacc gcaacgtcag aggggtcaac
     7081 acgaccgttg tctgcagata aggggatcgg aagaatgcgc cggcgtcagt cgccagttgg
     7141 cgaattcttc cccaggcgag ttcatttatg tgccgtgttc gttcgtttcc cggtcgccca
     7201 ttgaattccg caatggcatt tgccactgcc catgccgctt caggaagcag tactgcgtta
     7261 tcgtgcaccg tagcatatgc gccaactaag cgctgaactt caatgccttg gctaccttga
     7321 gcccaaccca caatcttcaa ctcaaaatct gtatccgata ggattcctct gcagtcaata
     7381 attgggttca aatttatctc tgatggtaga tttaactcat taacaactcc ggtatgctct
     7441 ggcgactttg ccaatgaata tacttggggc caaggtagtg tcacaccagt tgtgtcgatt
     7501 tggcatagcc cttcctgtcc gagttgtgct acgtacatcc ccaagaatgc ttcttgtgcg
     7561 gacgcttcgc tcgctgcttc gaacagtgaa tcggacgtgg cttccacgag aggcttggca
     7621 aaatcgacac gcagaccgtc tgctgtagca gcgatggaca gtacggggct accatcgctt
     7681 ttatcgctct ttcgtttaaa aaatcctgtc cagggcatca gtcctcgggg taataccaag
     7741 cgtttcggct tttcgaccag cggaaatccc atcgtttcaa tgctgtacac agggaagcgt
     7801 gctgttgtga gtcttccacc cagagacgcc ctccattcgt tctgtagtcc ttgatgtgcg
     7861 cctccgagta attgcttacg agtttcctca aattgtcgat cgagaacttt gctcttttgg
     7921 gggcatcgtc ggtttgaatg gcaccaattg aatgcctgtt gttagaggta gccggctgac
     7981 cgattctatc cgcaagcttg ccgccgacca agcctactcc tccaatcgaa tgtttgtccg
     8041 gatagatacc gagggatttt agcttatcct cagccttttg ttcccaacct aaaacatgaa
     8101 taatttgcag ctgggcaccg tcataaaaac catatttcag atcacctgtg ctccctgcat
     8161 atcgacgcgc atttgaatca aatcgcaact gtcttgtact gtagacgtag caagcgttag
     8221 gctttttgct aaattcgact gcaaggtagt caccaatctg catcatgaaa gcatcaacat
     8281 ctttgttgag tgtgagagtt gcataggttc cttcttcttg tgccaaaagg cttcggattt
     8341 cctgattgct gtgcgccagc tccatggtct ctgaaccaaa tacaagacgc gtccacgtaa
     8401 tctggtggag gtaacgcccc caaaattcga ggcgcccctc gtcgctgcct gacctaccag
     8461 cgagtagctc aaagaatagt tttaggttgg cttcgttaac ccagccaagc accatccgcc
     8521 agacaggttc ggaaacccga ttccacgcgg ttgccatacc ggcagccttg agttttggat
     8581 tacgccaaac gtttttatgc accacgaagt cgcgtaattg gggatgaggc tccctggatg
     8641 agctgcggtg atatcgttcg agaaggattt caagcgcctc gtctcgaaac accggtcgag
     8701 tgctgatcaa ctcaagcagg cgaggaatac gggccttaaa gtccgtatca catgtaaagt
     8761 cgcaacactt gcgtactgca cccaagacca gggaatgcca gaaccagctc gtctgcggga
     8821 taccaaggtc ttctgccagt cgattaatac tttccgtctc accattaagg tagcttactg
     8881 aatacttatc gacagccgca gccgttagca attgggactc acttcgcatg accttgatcc
     8941 agtcaggcac gatggcaact gcaccgtctg tatcaatatc cggccacgtg cgcctcaaca
     9001 tgttccgtaa tttgatccac ccttctcgtt cgctatccga tgccttaccc atatcaaagg
     9061 tgaaataaga tgagagcagc ccataccatg tcaaccgcca gagattacgg ctcttcgcct
     9121 ggaactcgta gtggttaagc aagctatcaa atcgactgtc gccaactacg caggtattgt
     9181 tcaactctcg aaaaggttcg cacaacgcgc tggctgctag atcgtattga tgatcggtca
     9241 aggactcacc gcgcatgaag gaaaacactg ccgcgctggc gtcttgtctg cttggacgag
     9301 cggtcgcgat gccctgaaac actatttgcg cttgcttcga ggccttttcg atctctgccg
     9361 gtcgaccaaa ttcgaccatg ggaatgtgga gggacgccaa cgcgctatcc agtgcttgtt
     9421 ccaacgagcg aacagcgtcg ttcccactca acatctcccc aactccttat cggaaattgg
     9481 agaatcggct ggtttttctt cgtcgagtgc ccaaaagtcg attttaaatt ctacccgacg
     9541 gctttctttc ttcgaggttt tcattgaatt aaaggaatac ccacccacta aaaagagatc
     9601 tcgaatctgc cgcttttgtg cgtccgctag tgggatctcc gcgcttaccg ttggtgcgaa
     9661 caatacacac accacctgcc tgctccggtc gagacttagt ccgaggttgt agagatatgt
     9721 tccatcctga tcagtgaatc cttcaacgac gatccgccgg atccattgcc ggcctatgtc
     9781 agaattttgc gcatcaagta gcagtggtac gtactcgcgc aggaagcgag catcatccgg
     9841 ccgaatgtct gcgcgaccgc tatcgaaaag cactttttcg cctaaattaa tgcgatacgt
     9901 gctttcatcg acacgaacat cctgaaagat gcgagatttc tcattgattc gcgacatcaa
     9961 ctccttaatg tctgactcgc gctgaatttt tcgctgttct tcgacggtaa tgttcttagt
    10021 caccgcgatg agggtcacag ccatgacgac taaaaacaac atcatgagtg ccgtcatcag
    10081 atcagagaaa gaaatccaaa atggactttc gccctctgtt ttaaagcctc tcttgagcac
    10141 cactcgactc agcatgacta cctgttccga ggaattttgg agagcgccat gccaagctcc
    10201 tgaatcacgc cggtaagctg attcatacca ttgccgaggt gagtgtcggt ctgcttgata
    10261 gtactggcca cctgatgact catctgggat ccaaaatccg tgaacgcctt tgcgagtgca
    10321 ccgttcacct tttcgagata ttgttggctt tgggtctcag caatgcgtaa ttgtccgaca
    10381 acgcgttcta gatcagcaat catctgcgtg gtgaaaccaa cttcccgctt tgcattttcg
    10441 atgagcgctg tgagcatctg cacatggcta tccaccgtct ttctagtatt gtcgtatagc
    10501 tcatagccct gcttaatcac taaagccgct gactgaagag tagttgcggt tgcctgtagc
    10561 tgatctgcca ccatctccga ccgttggaag acggcactta cagaatttcc tgctgtctcg
    10621 aaccgttgcg ctgcagatcc cattgttaaa gccccttgat tcatcccatc aatcgcgcgg
    10681 gtcgatgccg cattgatggc atcgatattt cgttgcgtgc tcttaacctg ttcggctaca
    10741 gatcggagca atgtatcgag ctgactggag agaccaccta ccaattccgt tgtgcgattc
    10801 tttaattgct cttgacgacc agactcctct tttacagacg cattgcgttc gtgcttcaga
    10861 tgctccatag ccgtcgacaa ctgaatcaat acattcgaca tggtctcgtc cataatgttt
    10921 tttgactttg cctgctcgtc agtgacaagc cttctgaatt cctgcacgaa ttcccgcatt
    10981 tggtctgtca tcaactgctg gttagcagca gcttgcctca ttgcgtcttc caacttctgg
    11041 ctcatttgca aggtggcatt ttcggtgctt ttctctatat tttctagcaa cctctgaagc
    11101 gaaacttgga cacttgccat ggcccccatt gagcgttcca tgctttcatt gatactctgc
    11161 atctgcccac cgaacgtgtc ttctagcctg gacataaacg cgacgagcac actctccagc
    11221 attccatgca cagcgctacc ttgctcaccg gaagcacgct tcaccgcctc accgatgtcc
    11281 tgcatcggct tctgtaagcc ttccaggatg gcattgccta attgattacc gagcgcttgc
    11341 gtggactctt tctgggcctc aatctgacgg tcgaccaagt tggtcatcat ctgcttgagg
    11401 tcttcaacca gcgcctcctt caactgagca gcatgggtct cactcatttc agccgaatgc
    11461 gtaagtctgg ccagatactc ttcgccagca ccagtgtgat acatcccgtc gataacctga
    11521 ttgagttcct cgacctttcc gtagaagagc gccaatacta acttctcgat aaataaaacg
    11581 atcatcgcgc aactgatggc ggcggctgat gctatgaatg catgagcaac accatcgagc
    11641 aaaggtttga gaccagctac cgcagttgac gttgtcgaag ggttgaactg tgagagacca
    11701 ttcagaaggc cagcgaaagt cccgatgatg cccagccccg tgaggacgcc aggcaaatgt
    11761 cgcacaaaat cgtcgaacat ccggctgtcg acgagaacat ctcgcgtgaa gaagatctct
    11821 gccggcatgg tagctcgaac ctcgaaaagc gtaacggcac cacttgaagc ctttgcaagc
    11881 gggtggagcg tgtctgcata ctctgcccaa aggtgccaga aaggctcatt acgcaatagc
    11941 ttggcaattt cctcaggggc aacggccttt ttggatgcag aaaggacgcg aattcccctt
    12001 accgctgtcc agagctgcca agcaacccga atcccctgca ggaaatatcc gacaaaaaac
    12061 gcgcccgcca gagccaagac caataggaaa atgtacagtg gaactccggt caaagtaccg
    12121 aatgacggat gtacctgttc tagcaatgcg aggagtgatt gagcgttcat ttcctcctcc
    12181 tgagcgccca ggaattggaa gtacccttac cgtgttcgga ctgttcggaa gaagattgtg
    12241 ggttggctgg cacgcagtgg tccatcgaga tcatgatgtt gttcccgggt ggaataggca
    12301 aaacataggc tcaggccagt cgtaaaaccc atccgacttg aaactttacg cgatttccaa
    12361 gaagcatttc gtgccctatt cgggggagat accagcactt ccttgcgtct tgattggagg
    12421 aaaaattcga ttcgccatcc gccaagacca tatctacgca agagcgaagt ctgcatacct
    12481 gtttttgtgg cggaagatta tgccattcgt catcgggcat ttcttcacaa aatactcacc
    12541 ctcacgattc actcggatct gcacgtatat tttacatcca cagcaggtac ttttacgcgc
    12601 caaagaatca cgtgctggtc ccagtccacg ccatcaatct tccgatttaa tagtgtttct
    12661 gcttgataat ctatttcagc gactcgacat caggcatatg ggcatgcgac agattgactt
    12721 gtaatatata gtagactata tattcattat catggaggat caggtgaaaa cagatgatac
    12781 caagaccgaa actcttcaat tcaaagtcac ggaaaatgag cggaaactca tcgaaaaatg
    12841 tgccgccgaa gaaggaacgt ccgtctccaa gtatgtgcgc ggagccgtgt tgatgagcat
    12901 ggtcatggat ggcaaagccg aggcgatcaa gatagtcgcc cgagaggtcg gagagaagac
    12961 ttttgccgtc gtccgacaga agttggcccg accgacacag gaaggacgtt aggaagagtg
    13021 ctctccgcct cacaactaac aggcaaagcc ggtccgcgcc caatgccggc aggctggggc
    13081 gcggagcgga atggcccccg tcttggtaac acgggggctc tcaccacagg gttccttaca
    13141 ggagttcgat aggtttcatg gagtctacat tcactctcac ggttgattgg ttggctttta
    13201 ccgtcttggc cagcaatccc caagaaacta tgaaagtgct tggcggagac tggagcaggg
    13261 ccaaaggtgg cttccgaggc tatcctctgt cctggatcaa gtccgatgga acacaggggg
    13321 tcggcaaact cggcacgaat gcgcctcgtc ggcccaatga gatccatgtg gatctctccg
    13381 ggggcctagt ctctgtccta accttggagc agattcgcgg cctactcaga tgggtgcatg
    13441 agcaacaagg ccatgtcacc cggatcgatt gtgccctgga tgatcgaacc acgagcgttc
    13501 ccatggccac cattcgacag gccgttacag acggtcactg cgtcacacgg gccgcgcaag
    13561 tgcggcagat cgtctcgaac ctcacccgtg gcacgggtgc gacgaccggc gaaaccatgt
    13621 atttcggcag tccccaaagc caaactctct tgcgtatcta tgacaagcgt ctggagatgc
    13681 agagcaagga acgggagaac tggcaggagt tcggcgtgcg ctgggagttg gaattcaaaa
    13741 aagaccgtgc ccaactctgt gcccgagccc tggccagttt ggacgaaacc gactggaaag
    13801 agtgcgtcgt aggattactc cgaacctatg tcaatttcag acagattcca aaagatcctg
    13861 aagaggaaga acgctaccgc gctccgatgt tggagtggta tgtcctcctg acggagggat
    13921 ttcagaaggg gcgattagcc caggagaagc aggtgcagac cctgcagaat gtgaaacgat
    13981 gggtgagcga caccctgacg ccaatgctgg cggtcatatg tgccacccct ggaggggaag
    14041 aatggctaca gcacgaaatt gtcaggggca tctcccggtg gaaggaccga caccggaact
    14101 tgctcaagca accacccaat cggtttcacc catcgtctgc cggcgggaac gcgggcagcc
    14161 catgttaggg ggactggggg tgtcgagaga ctctcccggt gatctgcacc atgcttacga
    14221 ccaaagagct ttcggcttgg cttaacatca agtcgtccac actctacctc tgggtctcac
    14281 agaacaagat tccgtactgc cgcattcacg gcctcatccg attcgagcct gacgccatcc
    14341 aggcgtggtt gaggggtttt ggagcacact cggtcaagcc gctcccgctg ccgacccgcc
    14401 aggattctaa cgacgtagag aacctgattg ccgcggccaa acgtgccgtc tatacttccg
    14461 gccacgggga aaccatcaca ccgagcccac gcgaaaagga ggagcagcat ggggctcgtt
    14521 aaacgaggca cgacatggtg gatgtcattt acgtatcagg ggcaacaaat ccgaagatcg
    14581 acaggaacgg ctgacaagcg aatggctgac gcgattctcg gcaaggtgaa ggctcagatt
    14641 attgaagggc gcttcttcga gaaaccgaaa gaagagtctc gaaccttcag tgaattaatg
    14701 gaccggtata gcacggagca tgcggcaaga cgcgccaact cccggcggaa tggcacgagc
    14761 gtgactcatc tccgcgcgta cttcgggaat ccaaagctga gccagatcac accgaaacac
    14821 atcgtggcct ataagaataa gcgctatgcc gatggggtca agcccgccac gattaaccga
    14881 gaattaagca cgctgaaaaa ggcctttaat ctggctcgcc gagagtggga atggtgtagc
    14941 gacaatcctg tctgtagagt ctccatggaa cgggaaaaca atactcgtga tcgctggctc
    15001 acgcttgaag aggagcactg tttgcttcag gcggcattgc cgtggctgcg agaactgctg
    15061 gtctttgcga tccatacggg gatgcggatg ggagagatcg tgtcgttgac ctgggctggt
    15121 gtcgatctga atcggcgcag cgtgacggtc tttcgatcca agaacggaga acggcggacc
    15181 attccggcta atcagaccgt cctcagatta ctcggtgaga aacacgccgt ccgtcagact
    15241 gtgaacaatt tggtgtttca tagccagact ggcacgcagc tcgatggaag caacatccga
    15301 cgatgcctga acagcgcgct caagaccgcg aagattcatg atctccattt tcatgatctt
    15361 cggcacacgt tcgcgacgcg catggtccag gccggagtgg atctttacaa ggtgcagcgg
    15421 ttgctagggc ataagtcgcc agtcatgaca cagcgctatg cccatcacta tccagaaagt
    15481 ctgcgggatg gagtggaagt attggatgtg cggcgaccga ttagcacaaa tttagcacag
    15541 ccactgggtc ggatcggtga ggtctctcta acacattgat tttatggagc cggcgagtgg
    15601 aatcgaacca ccgacctgcg gtttacgaag cgatcccgaa gtaatttcac cacctcattc
    15661 cacaccacaa aaacacgcag aagagcctga tccaccattg ggttaggtgg gccgtgcttc
    15721 atcctgtggt gttgtgccta aaaccgccga aagcggtctc gacggttaca cttttggtta
    15781 cactgcacgc ctcgcttttt gcggggcgtg caacctattg gggtcgggtc cgcacgcgga
    15841 ttcgagagcg gtctacgatg atgagagagg tagtgagctc agcttcggtt acctcgctga
    15901 gcaaacgtcc caaagcctgt tcggtttctt cgatcgttgt cggccaaacc cgaaggcgca
    15961 caatgccatg gtggcgctgt gctggaaaag atcggccatc agcgaagtcc tcatcgaagg
    16021 tcacaacgac agcggtctgt tgctgcgccc aggcgaagac ctcttcatca gccttcccgg
    16081 acaatcccac atcaccagtg tggaagacgg cccaggtcgg cttcaacagc ttcagccatg
    16141 aaacgacggc ttggggaaca ttttggtcga gaaggatggt cagacggtca ggcacgcgga
    16201 atgactttct cttcgtcaat cacctggctg gcatactgaa gcgcggcagt gacatcgttg
    16261 ttggtcaatt cggggtacgc ctcaatgatc ccagccatgg tatagccacc agccaccatg
    16321 ccgagaatgt ttttcaccat aatgcgggtg cctcgaaccg taggctttcc gctgcagatg
    16381 tttggatcga caacgatacg atcatccatg acgagtccct cactgactaa ccggttagtc
    16441 agcataaggc taggaggttc caggtgtcct gtcaagataa gccgctcgat tcaaacagca
    16501 gcaaccaaaa gccggacgcc cgtgcgcggg gtggcgcaag ccacccgcgc aggggagccg
    16561 cgccagcggc cactcctaaa cttgacagga ggacatttct cggcgaacac acttggcctc
    16621 acatcacatt acctcccgcg ggaaatgtct ggtccatcac gattcatgat ttcggaaaac
    16681 aatgggtcga aatgtcttgg ggaaggttag acccgattca aggcaagaga ggagaaaagg
    16741 gcgcatcgcg gaatagaaag atgaatgagg ctcgggcacg aggccgggca aaaagcacga
    16801 ttcgaaagaa atgcctgacc atcggagccg accatctcgt gaccttgacc tatcgagcga
    16861 atgtggaaga tcgataccgg gtgttacatg atttggaaag gttacggcgt gcgctctcac
    16921 ggtccggctg ttctatgccc tacgtggcgg tcctggaacg gcaacaacgg ggggcgctgc
    16981 atccgcacct cgccgtgaaa gggtttcagg atgttcggct cttgcgacgc tgctggtaca
    17041 agatcgtggg aaacggtcag gggcaggtga atgtgagagg ccctcgcccc gggagttccc
    17101 ccgtcaagct cgcccgctac ctatcaaaat acatcagtaa gagtttcgac aatatgccgc
    17161 gtgaatttga ggagcaccga tacttttgtt cattaggcgt caaggtgcca acggaaaagc
    17221 atgagtttgt gctggaccga agggcgaagg acgttgaaag aaaaatgtac agcattattc
    17281 ggcaagaggc gctgcgtcgc gtcggcgtct gttgccgtct tacggagtgg atgggtggat
    17341 cgggcaccta tggatggatg tctgggtttg aagatcgttc gatacaatgg gttactggaa
    17401 attctgaagc tgtgccgatt actccgagcc cttgactcac tgcgccggcg cttgctgggg
    17461 cgcgggcgct ctttgggggt atgggggtgt cgaagactcc cccaggacag tctgaaaagc
    17521 gcatatgtga gggttacaag ttccaacgga agaattcgac gtcgtcttgc ctccccgaat
    17581 cgggcaaaga tgggcactgc attgaggttt tctctctgtt ctgaacggct gcagacgacc
    17641 caaagcagac ctacacgact gaccactacc agaagggggg cttacgccga tccgcatacg
    17701 acgacgatct caccgcagta ctagagcagg aacttgtcct gcggcaatgg gttagggctg
    17761 gcgatcggtg cttgccgcct atttaggcag aagtggacaa tctgccgtaa cgggattata
    17821 caatccgtcc aaacattgtt acatggcaat gctaggttcg acttgattca tgtcagaccc
    17881 ctttcgacat ctgcaaagcg tcatcgatga cttgaatctt tctctctcga tctatgacag
    17941 cttcacgtcg cagattctca agtccacgtc ggccattgcc gcgatccaag actcgcttgc
    18001 agctcagcgt gccatagatc gtgacctgat ggcgtctgcg gcttcttctt tgtccgctgt
    18061 gcgtttacct gacttcacca gcaacttcag agacttggtc tccgccaaca aagccctcac
    18121 taatatcgtc tcgcaatcgg tgataacgga ttccgcgcta tcaaaagtat tcgccgagca
    18181 caacgctctc cgagattccc tagcgtccat cgcaacgttg ccttccgcta cagatctgct
    18241 ctcgtcgctc gataccactc gactacttca cacatcactc agcagccaat accgcttgct
    18301 tgaccttgaa acacactcgt ttggcagcct gattgatgcc tccaacgttc tttcaaacga
    18361 cctcgccgca acattcagca agctgactcg ctcataccgc gatgtgatcg aggccattcc
    18421 agttatccca caggattttg ttccgttcat tgcgacgtat tctccaatgg agtattcgct
    18481 cgaactcgat gtcctggagg gcatttcggt tgagcgcgac gaagaggccg aagttgaacc
    18541 gttgccctca gtggatgatg aactggcgtc gtttgatggc cgattgctga ctctactcaa
    18601 aggggcacgt gaatcactaa attcagacaa cccggacaag gctcgtcacg ttacgacgtc
    18661 cgtccgcgaa ctgtttactc acatactcca cggactcgct ccagatgctg aaatcacaaa
    18721 atggtcgacg gacgctggtc attaccacga caaccgtcca acgcgccgtg cacgccttct
    18781 gtatatctgc cgcaagtttt cctgtgaccc gttgacaaag tttgtcgagg atgacgttcg
    18841 cgccgcactg gcattggtga gctctttgaa tgccgggacg catgttgttc aatccaagct
    18901 gactcaattt cagctgcagg caatcgtgta ccgaatggag tcattggcgc tgttcctgct
    18961 caaggtatcg cgaggcggtg actagctcga aagccatgta acaatccgtt gcacacggag
    19021 ctgcgagccg tgcgagctct gaatcaacat cgttgcccgc ggaccggtga gcggggacgt
    19081 taatgcgaca gggctgagga ctgtttaatg gcggtacgat ctcggtgccg aacttccgca
    19141 cttggctgat ggcagtcctc gacccttagc ggactgttga cttgaccgga tgcaatgcta
    19201 cgatgcgaga aactgaccgt gatgggatgg ggaaaaccgg tcgattgtga acccatatca
    19261 cggaggaccc aatatgagtg cccatgatcc taggcgcgta gtagaagacc ttcgcgacca
    19321 ccttgccgcg catgaccgac ggctggcatt tcttctcggt gcgggaacat caagcgcggt
    19381 gaatgtcgcc cctccaccta caccaggtgc gaagcccgaa cacaaaccga tcattccagg
    19441 tgtcattgat ttgacgacaa tctgtgccac cgccgttagc aaaattggca acccctacag
    19501 cgcggcgtgg gaaagcatga tcaaacagtg tgagaaaggc ggtcgtaagg ccaacgtgga
    19561 aaacgtgctg tcgcaagtca gactaaagat ggacgcgatc ggtgaagggg aaaccctagt
    19621 tgggctgaac cggttgcagc taatagaagt tgaaaagaca atctgttcca ccatcgccga
    19681 cgtggtaagt ccggcggaca gcatcatctc acaacgcatg ccccaccacg attttgccga
    19741 ttgggccaag aaagtaagcc gcaccgcacc cctagagata ttcacaacga actacgatgt
    19801 tctgcttgag cgagctcttg aagcatcggg cgttcccgtg ttcgatggtt ttgtcggaac
    19861 tctccaccct ttcttttatc cggaatgtct ggaagatgaa gatttgttac cgaagccgaa
    19921 atggattcgg ctttggaaat tgcatgggtc agtaaattgg ttgctcgagg ctgtggaaag
    19981 cgtaggtgcg acgcgcaagc gaataatccg ggggcaacct acgaaaagcg gcgaactgat
    20041 tttgccttcg catcgaaagt acgacgaatc gcgcaagcaa ccatacatgg catatatgga
    20101 caggttgttg cgaatcttga gcgtggaaca tgcactcttg attacgtgcg gatacagttt
    20161 tggtgacgag cacatcaatg cgatcttgta cggagcattg gacaatcgaa acacggcaaa
    20221 cattatctcc cttcaatttg gggatttgaa agagacagac gatctcgtca aagcggcaat
    20281 gcaccgaccg aacctgacgg tgattggacc aaacggtgcc gtcatctcgg ggatctgggg
    20341 cacctggcaa ctcactgaac ccgtcgatgc caaaacgagc tcattcatgg atgcggcctt
    20401 tgatagcaat gccttgcccg aagacgaagg ttcgccggca accgcctcgg cggacctcaa
    20461 gggaacgatg cgtttgggcg acttcaattg gctttgtcag ttccttaaag ctatgggccc
    20521 gggaattcaa tgaaacaggg gattccaagt ctgattggac acgtggattc cgtgaaaggc
    20581 ggcgttgtga caattcgtct gcgtgacgac attccaactt tcatgatggt ggatgggcgt
    20641 tcgtatcgag taggtcaggt cggagcgttc cttcgcatcc cccttggcta tacacagctg
    20701 tacgctgttt gcacgcttgt cggtgcggcc gccgctccga agagtgatga gccgagcatc
    20761 ctgcccgggc accgctggct ttccgcgaca ttgtttggag aggcaattgg aggagttttt
    20821 gaacgaggcg tgagtcagta ccccactatc gaagatgaag ttcatctcgt aacattgcag
    20881 gacatgcacg tgatatacgg ggcgaccaaa gaagaacgtg cgatcactat tggcaccatt
    20941 gctgcgacgt caggaattcc gggcagtttg gacctgggtc gacttgtttc ccggcattcg
    21001 gccgttgttg gatcttcagg atcgggtaaa tcgaatttgg tggctgtgct tgttgaggct
    21061 atcgctacac agggttttcc ttcggctcgc gtactcatta ttgatccgca tggcgaatac
    21121 gcctctgcag tcggacagta tggaaaggta ttcaaaatca agcccaacgc aaacaagggc
    21181 gagctacccc tgcatgttcc atattgggct ctgccctttg acgaactgca ggccatcgca
    21241 ctgggttcga tgcagccgag ttcagaaact gcggttaggg acgaagtaac agcgcgaaag
    21301 aaatcatctg cgagacatct tgctagtgcg ccgccggaag cggtgattac tgcggattct
    21361 ccgattcctt ttagcataaa gaaactctgg ttcgacttgg atgactttga aagacagaca
    21421 tttcaagaca atactcggac gacaccatgc aacaagacac aggtaggtga tcctgagaat
    21481 ctggttccca atcagtatcc ggctccaaat cctgggagcc aagctccata cgcacaccct
    21541 agacctcggg gcattgcgaa acagctggag ctgatgcgaa gtcgaattca agactcgcgc
    21601 tttgcattcc tcttccatcc cggcgatgag ctatctccaa atctcgacgg tgaaactacc
    21661 ggggacctcc atagactcgt ccagtcgtgg gttggtcacg acaaaccgtt gacggtcttg
    21721 gacgtttcgg gtctgccctc ggaagtgttg tccacaattg tgggtacgct tgtaaggatt
    21781 gtttacgatc tgctgtactg ggcactcgac ctgccaacta gtggacggaa ccagccactt
    21841 ctgattgtcc tggaagaggc gcatatcttc ctcccgcagg gtggcgattc tgcggcgcat
    21901 cgaatgatat cgaagatcgc gaaagaagga cgaaagtacg gggttggtct atgtgttgtg
    21961 actcagcggc cagtggaaat agagagtacg gtgcttagcc agtgtgggac gatgatcgcg
    22021 ttgaggctta cgaattccgc ggatcgtgcc aaggtggaag cggcaatgcc tgacgattta
    22081 ggggccttgt ctggcatgtt gccagccatg cgaacaggag aaggaatcgt actgggagaa
    22141 gcaatgccga ttccctcgcg aattcaattc tacaaggcgc gcaagcgccc gcgaggcgac
    22201 gatcccgaaa tgccgcaggc atggcggcag ccacgaccgg acgtcgttca ctacgaatct
    22261 gccttgaaca actggcggca tcaggctgac atgactggag aggagacgaa cgatgcctga
    22321 aatgatttac gtcgattcat cgaacatcga ggcagttggc tacgatgctg gttcacaaga
    22381 gctccatgtg caatttcttg gaggtggcca ctatgtctac caccaagtgc cacgttatat
    22441 ttttgacgac tttctaagtg cctcatcaaa agggagtttc ttgaaccggg aaatcagaaa
    22501 cgtgtaccaa ttcacgaaac agtagctgct cagactactt ggaagctcga ggctaatttg
    22561 tccttctttc attttgcgcg cacatacatc agacaattcg aaggctccgc acaatgttcc
    22621 tgacaaagaa tgatttcctc gtggctcgga cttgtccaac aaagctttat tacaagaagt
    22681 tcggttatgc gtctatcttg gatgacgatc cctacaggga gttccttgct gacggtggat
    22741 atatggtcga agccatggcg aaactgctat tcccggacgg ccgagaggtt ggtcagtggg
    22801 aggagccgat gagagcgttt caggaaactc gcgaatcgct tgcaggtgga gatacgacgc
    22861 tctttgaggc aacggtagtg catgagaatt tgttaacacg agttgacatt ctgcggcgcc
    22921 aaggggccgc gttgcatgtg atcgaggtca agtccagttc atttaattct gaaaccgatg
    22981 gggcaaaccc atttcgtggg aagaaaggcg ggattgcgtc ggagtggagg gagtatcttg
    23041 aggatattgc atttcagacg gtcgtactgc agcgggcatt tccagaatcg aaggtagtac
    23101 cgttcctctg tatcgtagac aaggcaaagt cagcgactga gaacgcgaca ttcgatgatt
    23161 tccgggttcg tcgttcgagc ggagccgcgg gtctcgccaa aacttcagtc gagtacctgg
    23221 gaaatgcgga cagtctgcgc aagcagcatg tgctcgccat cttggacgta acatctgagg
    23281 ttggcgaaat tattgccgat gtagcagcag cggctgagga atttgcaaaa acacttacat
    23341 ccagcccgat tcagaagatc ggaccaacaa tcggggtgaa gtgtaaaaag tgcgagttcc
    23401 gcactgagag aggcgatgag cagaccaacg gattccacga gtgttggggg cctctggcgg
    23461 aagtccagcc gcatgttctc gatctatttc gtgtggatgc gctcggcggc aagaaccgcg
    23521 atgtagtagc cgagatggta gcgaagaaca cggcgctgct aacggaagtg ccgaatgatg
    23581 ctatgcaagg cgcttacgcg gcccgtcaat cgtttcaaat cgaatacacg aaaaataatc
    23641 gagaattcat ttccgacaaa cttccagcca ttttggcaac tcatacctat cctctttact
    23701 ttatcgactt cgagtgctct cgcattgccg ttccttatca cgccggcatg cggccatatg
    23761 agatctctgc ctttcagtgg agctgcaata ccattagtca tcccggcggc gaggtaaaac
    23821 acgcgggatg gttgaacgcc gatgaagtgt tcccgagctt tgagtttgcc cggactttga
    23881 aaagccagat tggcgaagaa ggaacagtct atatctggtc aaattacgag attaccgtgc
    23941 tacgggagat tcggcagcaa atggattcgt atggtctgaa tgagccggaa ctgggggcgt
    24001 ggctagatcg ggtgactacc aagggaaatc cgagagttgt agaccttttg aatttggcca
    24061 aggaccacta ctttcatcct ttgatgggcg gcagcttatc gatcaagtat gcacttagag
    24121 ctgcctggac ttccaacaac aagctacgcg ctaatcccgt gttcgcgaag tatgtgaaga
    24181 cagacgaaca tggacgtttg ctcgatccat acttgtcact tccgccgctt ccgattggag
    24241 agaaagagga agtggtgcaa gaagggactg gggcgatgcg tgtgtaccag gaaatgatgt
    24301 ttggctttgt aaaggaagac gcaactcgtc gcgagtctta ccgaaagctg ctacttcagt
    24361 attgtgagtt ggatacccta gcaatggttt ttctgtggac gcactggttg acgctatctg
    24421 gacaaaaaga aattactccg agctgattca agttgaatgc aaacatgatt tccgattcaa
    24481 atcaagacga attttggcca agatgcagaa taacaacgta gcacactgga cactctgtaa
    24541 acgggtttct atcttcagcg ttgaagatcc gctttgtaac ggtgtgatct cggtgccgaa
    24601 cttccgctcg tggccgatgc cagtcctcca cccttagccg aagtacacga ccgaccgcta
    24661 ccagaagaga gggtcttacg ccgatccgca taagaacccg tgataaatgg tcccaccgtc
    24721 gcaactcatt gaaacgacaa cgagcatggc atattttcac gaactgtgct ggcgtgttat
    24781 acggacagaa atggtcggat cgagcactta cgcggatccg tctaacattg ttaggacgat
    24841 ggaaagcaca tggcaacgct cgccgatcta atcccagatg taagtgccgt cctagcactg
    24901 gaacctgaag aactcgctgg cgtcgcactt gaactgctga cttcattggg tccccatgaa
    24961 ccatccaggc tgcacccgtc gagtttcacg aattcccaaa ctgtcgggaa gtatcctgac
    25021 ggccaaaaac acgagattga gcttgcgttg atggagggtt ggcattggct tgtccaagag
    25081 ggtttgatcg caccactccc aggagacacc ttcggctggc acttcattac tcgtcgggga
    25141 aagaagatca agaaccgtga agggcttgca gtgtatcgaa acgccgtgat tttgcctcgc
    25201 cgattgctgc atcccatgat cgtccgagcc tgctggtccg cctttcttcg cggagactac
    25261 gacacatccg tattccaggc attcaaggag attgaggttg ctattcgcga ggccggaaag
    25321 ttcaaggccg atgattatgg tgtcgatcta gcacgcaagg catttcatga gtcgtctggc
    25381 cctctcacgg accaatcaaa gcctgtttct gagagacttg ctctttcgca cttggtggct
    25441 ggtgcgctcg gctcgtacaa aaacccccat agccaccgca aagtagaatt gactgccgag
    25501 gaggcagtcg aaatgatcat attgagtagt cacttgctga agattgttga ttcgcgcgcc
    25561 aatcaccaaa cgtcagcgcc ctaacactgc gctcaaccgg accgtcgaga gccgcgtttt
    25621 tgccggccgg ttagctttac gttaaacggc gcgctcaccc ccggcttggt tcgaggagga
    25681 ctatgtgaga ctcgaaggcg ctaaagaaac cgatgtacgc gcagaaattg ccgagcctct
    25741 gctggctgca ttagggtacc ggagaggaac gcccaatgac atctcgcgtg aagtttcgct
    25801 aacgtatgca cggcagtccc ttggtcgaaa aaagcccagc gatccgccgc ttcgaggacg
    25861 cgcagatttc gtgctcgctg tgcttggcgc ggggcgatgg gtgttagaga cgaagccgcc
    25921 gcatgagcct attgacgtgg atgcgattga gcaagcaatt acgtatgcac gccatcctga
    25981 tatttcggcg gcatatgctg tcattctcaa cggggtgcgc cttaccgttc accatacatc
    26041 gcagacttcc gctgaaaagc cactcgttga tctgaccatc tcagacgttg cctccttagc
    26101 caagcagctc gcaggcttgc tatctcctgc tgcagtccgg cgggactgca ctccgccaat
    26161 tgtggatctc ggaagcccgt tggccgaggg cctccgttca cgagtatcaa tccagggtgg
    26221 ccagatccga cacggcaaat ccacctggtc ggtcaacatc cctctccccg atgtcgaagt
    26281 tcaacgtctg gacgagatgt gccgtcgtct gggcggcctt cgaattgcga ttacaggtgg
    26341 aactgtgtac cgcgattcgg agtcgcgcat ccgcgccaaa ctgcagtggt ccatgcctca
    26401 cgatgagctg ttgaggttcg ttttagacaa gaaattgatg gatatagagt acattgcact
    26461 tgctgacgaa atctccacag atccagacgc accatcagtt ttcgatgtaa ttggtgatgt
    26521 tgaagtgagt gagggggaga ctctctttaa catcgcaacg tgggaaacga caactgcggg
    26581 tgcctcgatt cgcatgcggt acacgggcag tgctgtaggg ttcttcgaga aaaacgtgtt
    26641 tcacggagtt ttttccgcac actactacag tgaggcgccg ctgctgccgg ggtttcgaat
    26701 ggcgatggag acgggaggcg tattcgaagt agtcgttgac gagaagtgac cctggccgca
    26761 ggttccgatg agcgcgccaa tgcgccgtct aacaggtcga ccgacacgga cgttccattg
    26821 atgggctttg ccctcctgct gtccgccggt catctccgac gttagatcgc aagaagagcc
    26881 atgaggagaa ccacgtggag cacaattttg agtatctaaa ggcccatatc ttgccgctgt
    26941 cgcgagccaa aggattcgat gttgcccgca ccgagtggcg ccttgttgcc gttgagataa
    27001 gtgaggaaat cgacaattgc ccatgcggcc aagagatcaa agagcattgc tacatcaaga
    27061 acagcgtcac cggacacgaa acctacgtcg ggaatgtttg catcaaccgt ttcatcggaa
    27121 ttgatacggg gaatctgttc ggcggcctga gacgcatcgc ggaaaacgac accacgaatg
    27181 cgaacgccga tgtcattgag tatgcgaacg agcgtggatt cctctacgac aaagaatacg
    27241 aatttctcat gagtacccgg ctcaagcgca cactgtccga caagcagaag gcgtggaagc
    27301 ggaagatcaa tcgacgcatt ctcgacaaga tagtcgtgca acggcgtacc agtcgatagt
    27361 gagttgcgat ctaacaaccg gcgggctgcg ggcagtccgc ttcgcggccc gcagcagaac
    27421 tgatgcatta gatgcaaaac atcaattcca aagatccgtt tcccatttac cgcagagtct
    27481 gtcgtgtgac agtgcgcact gacccgtcga tgattggcgg cctcccggtt tggtttattg
    27541 cagttcgttg accaagaccg gccggaataa tgctccttat gtgttaccct ttgtcgagcg
    27601 atcggccccg agtgccgttc tgcctctaca aggtggtggg cttctgcagg cctgagattc
    27661 atttgaatca acgctgcacc ttctaaactc ctgccacaac tgtgtcaatt cgtaggcacg
    27721 ctcaatagtc gtctgatggc cctcgctcgc tgcttttgct tacccctaaa aggagatgcc
    27781 atctcacaac cttggcaaag gtggagcgca cgcagagaat ttaagtttga ccgtgggatg
    27841 catcggaggc gggcgttggc cacccgatcc agcgatagcc ggacgggcgt aatagcccca
    27901 tctcagtaac acgggggcct aggtataccc ccattcccac cgcttcgcag cagacttgac
    27961 acacctatag ccatacgata tatgttcgca tatataacgg aggtctatag atgagtccaa
    28021 agcctaaaaa cacaccccat cgagttctat tgtcgttgcc tacagatctg tacaacacca
    28081 tttctcgtgt atccgctgcg atggaaaagc cgcgagccga aatcatccgg gacttattat
    28141 ctgaacaaca accgattctc gaacacatgg cgacgatgtt ggagcaagcg aaagcaggca
    28201 aactagatca ggccatgaag ggctggaaga aaatgaccgg ggaggcattg aaaggtctgg
    28261 gcgtgctcat gactccccca gatatcagaa aggcaaagcc gaaggcgtaa gcgggtgcag
    28321 gtcttctgtg caatacaacc atttcgaaat gaaagggggt gaggaacatg ccagagcaag
    28381 tgctgaaacg actgtattcc gtgagggagg cggctcgcta cctgggggtc agcccttggt
    28441 cggttcgcaa tcttcagaaa ttacggactt tgccctgtgt gcggcaaggc cgtcgagtgc
    28501 tgttcgatat tcatgatttg gaacggcaca tcgagaacca caaaacggag gtccatgatg
    28561 ggcatgattt atcgccgcaa gaaacgtgat ccggccacgg gcaagctaga ggaacaaggc
    28621 ccctactgga tgaagtatta cgttgagggc cgaccgattc aggaaagcac aaaggtcttc
    28681 gagaaggaac aagccaagaa gattctcaag gtcaaagagg gagaagtcgc ggctggactg
    28741 tatcgcgggc caagcatgga tcgcatcaga tttgaagacc tggcggcatt ggtccagcag
    28801 gactaccaga tcaacagacg aaaaacggtt cgacggatca cagagtatca gaaacacttg
    28861 gagccgtatt tccgtaaagc tcgagtgtct tctatgacca cagagcggat taacgcctac
    28921 atcatcaaac gacaggagca aggggcggcg aacggaacga tcaaccggga aatcggattt
    28981 ctcaagcgga tgttccgttt aggctaccaa cagactcccc agctcgtggc ccgcgtaccc
    29041 cacattcccc agctcaaaga gcacaacata cgctccggct tcttcgagca tgaggatttc
    29101 ctcgccctgc gcggggcact ccccgactat gcccaagttc cagccacgct ggcctactac
    29161 agcggcatga gaatgggaga agtgtactcg ctccaatgga ggcagattaa ctggacagaa
    29221 gggaaactgt ttctgaaggc ccaggatacc aaaacgaaca ccccacgggt tctctatttg
    29281 accggggacc tctatcgggt gttattggcc tggaagaccc gttgcgacaa gaaatggcct
    29341 tcctgtgagt ggatctgcca tcgaggcggc attcgcctgc agagcttcaa gcgctcctgg
    29401 tgggaggcct gtgacgccgt aggattgggg cgcatggtga aggatgagaa aaagggacgg
    29461 ctcgtctggg atgggaagag acctcacgat ttccggcgta cagcggtgcg aaacatgatt
    29521 cgggccggag tgccggaaaa gatcgccatg gccatttctg gccacaagac cagaagtgtc
    29581 ttcgaccggt acaacatcgt gaacgaacgt gacctcgaac aggcggcacg gtcactcacg
    29641 acctacttcg aaaagcagac ggttacactt gcggttacac tggcagaatt gaggggtgag
    29701 agcagcggtg tcgcaaaccg ccaacaggtt gattcgtcga ggatatttgt ggagccggcg
    29761 agtggaatcg aaccaccgac ctgcggttta cgaaggcgcc ggaaaaaaag aggatcacga
    29821 tctaaagcgg tactttagag agctcaaaca gtccggcagt ccctttgatc agacaacgtc
    29881 gcactcctac cagccgctca ctgcgcacga cttccgccgt attgtgttag gcgacgccca
    29941 tcagaaaacc aaactcgtgc ccatgcttca aatcgcagat ttgatgctgt acccgatcgc
    30001 gaaaggcggc tacgatcaca cgtacccacc gtatcaaaag ttacaacaag gagggaagct
    30061 gatcgacagt tgttttgtgg aggaagaccg accgttgaga ggaattaaat atagctgctt
    30121 tgagataacc cctgaaaaac ctaaaggccc ggatttctcc gagcctttat aagtcggctt
    30181 gcaagctgag cgcaaacccc cgggcattgc ccctatggat gtactatagc gcactaccga
    30241 ttcgttttcc agcctttcga acgactgcca tagtgcacgg gacgccagaa aagcctcgcg
    30301 ctaccgctcg ctgtgatgtg acattaaaat tgtagactta acctctcgct ccaacgcagc
    30361 atttgggtac gttgggatta ggtgcctggt atactctgcc ttacctcacc caccgaacca
    30421 ggagttcaga agcttttcat ttgaattcct gacgcgcgcc ccccaagaat gttgctagga
    30481 agggtatgct cttggtatac caggtactat ggagtcagac agaccattga tcacatccga
    30541 tctggcccca gctctgatcg cagcatttct gcaggagcgg gcggcggcaa ttgccgatac
    30601 cactgtcgcg acctatgcgc gaaagctcac cacgtttcac gaatggtggc gcgaccatga
    30661 gccctccctc cccgtcaacg aaacgttggc gacacggtac gcgcgctggc tcgcaacgcc
    30721 cagcacacgg agcaaggcct cacaaggcct gcatctgtcg gccgtccggc aatgggcctg
    30781 ttttctccta accgccggcc acctgtccat caatcccttt gcggctgtgt cagggccgcc
    30841 gcgggcgcgg tggttgcggc atcgcctgct cacccgtacg gatctgcaag cattgttgaa
    30901 gcagttcgcg gtgaccacgc tcgtggggca acgggactat ctcctcgctg cgttgatgat
    30961 tcgcacggga gcccgtgagt ccgaacttgc cacggccaat atcggtgatc tcacggagta
    31021 cggagaggaa gggagtctgc tgttgttgca cagcaaaggc aaagccaaac aggaacccgt
    31081 cgtggtacga ccggatctca gcaccaagct ttggacttat ctccgccagc gatatcctga
    31141 cggccgcttt ccgtcacaag acccgctgtt cacgaatcat catccgtctt cccgagggga
    31201 acgcatgacg acacggggaa tgcgcaagca actaaaggct gcatttacgg aagctggcct
    31261 tgcccaaccc catgtgaccg ccctgtcact ccggctgtct ggtggagcct tggcctatcg
    31321 gaaaggggcg agcccatctg agctcaagaa gaccatgcgg catgaatcca ttcagaccac
    31381 ccaggtcctc attgaccagg tcaaccgcat acggtttgga gcggaacgct atctggacga
    31441 tattcagtag acagcaggat gggaatcacc tggcatcctt taaggcgaga gccttgggtt
    31501 cgaaccccag ccgactcatc gttcagtaca aaagatggaa ctttctaaac caccatcttc
    31561 ttgtgatcac tgtagccagc ctttcttcca gtactcaaga ctcagtacca tgagaattaa
    31621 tcgccaggtg tcctaacgcg gacttgaggc tttctcgtag agagtgcagg aggacgctca
    31681 ttcagcgcag caggctggat caggcgaatg acggaacgaa tctagctaga atccatcaca
    31741 gtgagctgac aatgtcctgc cccagttctc ttcttatcgc gtaggcaatt ctcttactga
    31801 tcgaccatgc tgggtgtcgt tgatcactgc agtattgcca tctatcactt tgtactctct
    31861 gtaataagcc gcaccgcagt acagcttacg cttcgtgcca tctcggtcgt tctcacaggg
    31921 gctgttcgat acttcgcaag gaatgtcccc ccctacagtt ccaaactcat tggtacattg
    31981 aaacttattg cccgcattcc cacctttaac aaagaattcc catttgggtt tgacggtttc
    32041 agacacgggc acgcgaggaa tcgacaagac tcgactgccg tgtttttccg tcttcaattt
    32101 aagatcaatg tcattatgga tattggtgat cttgcaggtg aagttgatgc tcttgttgaa
    32161 aatctgggtg cctttctggg gcagatctac acagttcacc gcatccgccg aaaactcaaa
    32221 ggcctcgaat actgcaaatt caggggtttc agttggtatg aagccgatag cgacagaaat
    32281 cgatttttcg ctcttacgtt gagctttaaa ggttagtccg cctttgcata gttctagtgc
    32341 tgcgtcagaa atgtaagacg tttcaaactc ttggtggtga tcaagtagat actgttcatc
    32401 gtggccttga caggcattct ccatagtttg tcggttgacg gtgctgttgg ctggtatgcc
    32461 gtcaacaatc atgcttagac caagcctcca atccgattgc gaggcggtgc agtaatgggt
    32521 gcttagactg cggctttcct taaggtggtc gtagttatgg atcctggtct taagagcgtt
    32581 taccagatct ggttcgcaca tctccgcatg agatgggtca atggtgcagg ctaacatggt
    32641 aagcgcagtc aacaaccttg cagcgacgag tatgtgagga ttagaggcga gcgctttttc
    32701 tttttggcgg accatgtctt actccaaatt tcttcggtca ataacaggtg agaagcaaat
    32761 gcaatggtgc ggcttatagt agctcctggc agaaatgtca atttatcccc gcaggttttg
    32821 atagaggtat caaacctttc atctggatgg gagcgggtcc accaatatcc gttttcgggt
    32881 ctacacgaca tccagcagtt aggatgcgct cgcctacgaa tcactcgatc accggaacga
    32941 tccgcctcgc agccactctc accgctcgcc acgtgtactc atcatttcga tattgcaaga
    33001 gataatcggc tcggaccgca ccgagatcag ctcccgtcgc gccgagtctg actgacagct
    33061 ttcgcgagac aaaatccgtt ccgctatggc agttccccgc gtcactggaa tctgcagcgg
    33121 acacccagat gcgtgagagc gaagcctgga tctgcaacgc ttccctctct tctttcccct
    33181 ggcgtctcca cgctgtgatc gcctcccgta ccgcccggcg cccatcccac cccgcggcgt
    33241 gatatgagcg ctctccctcg accaacgtgg tgcggcgtcc gcacacccat ccccgcgtga
    33301 tgcggagtcc gtgaagatag gcaatctccg cggagaagcc ccacataatc acacctgcga
    33361 ctttcacttg gctgatgggg tgggctaata gccatctggc accaacctcg atacgtatcg
    33421 aggacgcgtg atacaacatg ggccatccgt acgaactagg acaatgcatc acgctgcgcc
    33481 ctttcaatga tgtccaataa tgcctactcc cccgttgaga gggctcagcc gcgttattct
    33541 cactgagcac cacgggatga ttgccggagt cttcagccca gaggcgggac actaatttgt
    33601 gctgcgcgct gagtgtcata tgcgtctcct tgtccgatgt gtaatgagcc aggccagctg
    33661 ggctcgcgcg cagctggatc agataaaaga tggagtgggg tgaggactag ggctgaatgc
    33721 tggattggca ttcaatcccc gaagtgcata taaagagata cgttgcggaa aaagtttccg
    33781 ccgattctca gatcatggga acttctgtag aggtaggggg agctcaggcc tgtcgttttg
    33841 acgagggcgt gatcaacgat caattgtgat gctgtatgga gatcgcgcga tgacccggga
    33901 tacagccatt ggtatcgtca ttcattcgtt agtcaccgga ttcctgatcc tggcagcgat
    33961 gcagggagcc gcatcagcga acggtgaggt gtgggtattg gctacctact tgggggcgag
    34021 tattggagcc accctctatc cgaatctcac gacacgggta tcggtgatgg ccggctctgt
    34081 catggcggct tttctgtggg aatttgtctt caatcatgtc gttgggaaat aacaggataa
    34141 attgctcacg taaagttcaa tgcctgcgtg aattccaggg actggcccag ttcccacgcc
    34201 tctctgtatt gggcggatgt caccgtgcgt tgggctttct tcagaatttg agtccgttct
    34261 gcagaatcag ccgggccaag cggcgagtga atcttgctgc gtatgtgatc ggccaatgcg
    34321 tagaggataa cagcattctc gaagttgttt tccgccactt tcaatctcgc gaagtattct
    34381 aagcattcag ccatgccgcg ttgatcccca atttgaatga cgagggcacc gcttttaccc
    34441 agtagggtat gtgcaacgtc gaactctttc atgccaaccc tcgcccttga tatcgtgcgt
    34501 agcgattcgg ccatgccttt tcgatcacca aggtcttccc tgatttgata gcttcgttcc
    34561 ccaaagtgta gaccttttcg gtagtatcgc tgaaagcagg cgacctcccc caatatattc
    34621 agcggaaacg cggcctccct catatttcct tctgacacaa acctctgcag gctctcttct
    34681 aaccgttcac gtgcatcgtc gagcttaccc aggtgccgtg ccaccaaacc actgttacat
    34741 agagccacgg caataccccg ttgatggcct gtcaaacgtt ccagtttgag gctttccgaa
    34801 aagagtcgaa gtgcttcttt atagttgccc ctcatggacg ccacattgcc catgtcggtc
    34861 agcgcagtgg ctatactcag ttggtcattc gtctgtttgg cgagatgtaa gctttgtcgt
    34921 gaagctgtat cagccgtgtc caggtcgcac tctcgatacg caagcgtgct caagccactc
    34981 aacgccttcg ctctgagtag tggaaatgtt tcagcctctg gcatgcgtag caccatgcgc
    35041 aatttgtcag atgcctgccg atagaacccc ttgatctcca tggcacgcca aagcgcaccc
    35101 gttattcgta gggcgagctc agcgtgatgg tggctcaccg agaaggatat ggcggagtag
    35161 aaattatctg cgtccgcccc taatcgttgt agccatacag actgatttgt cttgagcaat
    35221 tcaggtgccg cgtgttccgc atggtctgcg aaccagcggg catgcttttc agcaatggcc
    35281 tccgtctcgc cagcgcggcg tagttcatca gccgcaaatt cacggatagc atcaagataa
    35341 tagaatcgtc gttcccctgc cacgtcggtt gcctgcagga gtgacgcacg caataatgcc
    35401 ttgagaatct ctactacatc ttcttcacaa tcgtccggct cggcgcacac tgagaggacc
    35461 gcactcttgg tgcagcctcg aaaaatcgat aatctccgga agatacgctg aattttcttg
    35521 ccgttggggt gtcggcgaat caaatcacag ctgatttcta tagactgccg cagcgacttc
    35581 cctcgagttt cttcttcgtt cacatactcg tcgttccaca gatctaggcg atcttcaaat
    35641 cgttcacaga tttcatcaat gctcataaca tctaactgag cagcagctaa ctcgatcgcg
    35701 agggggatac cctccaactt tgcacagagg tcggacacaa cacgagcgtt tgcagttgtg
    35761 aggcaaaact tgcggtgcgc ttgggccctg gccaggagaa gtttgacaga atcgatagag
    35821 gccagctgtg taagactctg ccccacgtcg gaattgggca cctcaagcgg aggcatgcgg
    35881 taaatgtgtt cccacttaat tcctagatca atccgtgtgg tcaccacaat acaaagatcg
    35941 gagcaccctg acagcaactc gtccacgaac ttgcaacatt gttctaatac ggcttcgcag
    36001 ttgtcgagta caaggagctg cttcccaatt ttcagggtgt ctcgaatgag actgcggagg
    36061 tcgcgttgga cctgaccatc aatgtgtagc gcataagcaa tgcgttgttc taatgcaatc
    36121 tcatgaagag gaaggggcac gtaggagacc aggtggaaat caggcgtcac cgatcgagcg
    36181 atttccattg cgatccgagt tttgcccacc ccaggaggac cggcaatggt tactagtcgg
    36241 tgtgtcttaa tcaggtgtcg tagctcgctt tgctcacccg tgcgtccaat aaactcatct
    36301 ttgggcggtg ggatattgtg ttgccgttga accgccgcca ttggagctgc aacagactgc
    36361 acttggaccg gatcagccgt gccaagggtg atctcgtgca cgtgaacgcc caacgccttg
    36421 gctacaacgt atagggtatg cggataggcg cggcatccca tttcaagtcg ttgaatagtt
    36481 cgctttacga ttggcgcctc gattgagcca tctttttccc tcaacttcgc tacttcatcg
    36541 acaagcttgt cttgtgacca tcccctctgc tcccgtagtt gtcggatctt cttcccatcc
    36601 gcctgaggca tgttcaattc accagtttga gattacgcca agacgcgcca aggtgtggcc
    36661 gcgatgtcct gagttttttg gtactaatcg tcctaccgac aagacacaca tcgggtgatc
    36721 gaatcgagcg cggcgattgt acatacacac agacattcgt tcaattggcg ggaaacagac
    36781 acgacccaga atggagagga aggctcacac tgatcagagg ccagtttgag tagtcaagct
    36841 gagccgacat tatcttataa cccaaggagc gctgacgaat gaccaacgag tgtacgaact
    36901 gtcatggaag ttggttttgc taaagacgaa gtgttctgca atctaaccgg aggcacacat
    36961 caccaaaagg atcttagcga tatcccttct gtccgcagtg ttgtgaccag tcgttctaac
    37021 ttgggctgtg gacatgatga caatacttgc ttttactggg agatcgaagg tgatctcggg
    37081 tggcctattt ggcgaggaca cgatcgagtg ttggtatcga agtttggatg agtcctatac
    37141 cagagcgttc atcttcaaga gagattcctg tccaacaaga gtatttgctg atggtatccc
    37201 ggtgcacaac cttcgataga cgatctgcat aaacagcagc gatctggacc ggtcgatgag
    37261 accaggcaga ggagagcctg aatcaacctg cgtgagtcta tgaggtgagg aagttcagtg
    37321 ctcaaccggc catcaaggtc agtcatttcg gagtaaatgc cagcgaacag ggtgtcagtg
    37381 cgagaagtgg tttgcgtgga caaagcgagg gaacagcatg atgccgagtc tcattctttg
    37441 tgcagtcttg atgtctgtct tgggccgaga gcctgtggcc cattcaattc aacggtatgc
    37501 aatctccgtt gacggcacgg aagttgttga tcaccaaacg gggctggtct ggctgcgttg
    37561 ctcagaagga atgtactgga acggacatgt ttgcactggt acaccgggca gattctcccg
    37621 tgatgctgca ttccagcgag cgaccgttgc tggaattctc tggcgcgtac cgaatcagga
    37681 tgaactgaaa agtattgtcg atatggacag caatttggaa cttctcgatg gcgttaagtt
    37741 ggcagcaact gacctcgcca catttcccaa tacccctgca aatcactttt ggtcgtcatc
    37801 gcacaacact ggcgagggga ggtatggatg cgtcgatttc tacgatggcc ttagatgcgg
    37861 cgtcgagagt gtgcaggagc aattctatgt ccgcctggtt cgatccggca actcaggttc
    37921 ttatggtgtc tatcagcggt tgcgaaaaca agtgacacag ttaaattggg aggtgacaaa
    37981 tgaaaggtat cggcggcgtg ctgttagttg tcggcggctt tgtattgtac aactcctttc
    38041 aggcaaccca aacgattgca tttagattgg ccaatttgct cggcaacgaa gcagccattc
    38101 aaggggtcca acttgacatg ggagtcggct tcatcatggc tcttatcggt ggcggtctcc
    38161 tcacccgaga ttcctagatc gcagagctac actcaaatga cagcgaagcg gagcgtttct
    38221 tgccgaaaca aggaaggcgc gttcattgag gaagcgccgt cagaagtcag caattctcct
    38281 tcgagcgtgt gtccttgaac aagcagttca cgtgaggaca cgatccctac gaggcgtgga
    38341 acaaatgtgc caatatgata tacgtagttc acgccatact taatcgcgta atggaattga
    38401 gggtcaatca ccttttgagg aggctaaccg tgaagcatag ctggctcttg ctattacaat
    38461 tggtactggc aggcctgatc agcggatgtg cagccggcaa tggcaacagc acacacgaga
    38521 ttggtagtgg cgaactagtt accaacttag caacgctgag gccgctggcc gaccagggca
    38581 acagggacgc tcaatttaac ctgggcaagg cataccatat cggtctaggc gtcccaaagg
    38641 actacgaaaa ggccctgctg tggtaccgca aggccgccga ccagggtgag gccaacgccc
    38701 agaccaatct cggaatgatg tatgccaacg gtcaaggagt ttcgagagat tgtccacagg
    38761 ccgcactctg gctccgcaag gctgctgatc agagcaacgc cttagcattg accgcactcg
    38821 gctttcttca cgaaatcggc aacggcgtac cacaagatta tcggttggcc gcacagttgt
    38881 accgcaaggc ggccgatcta ggatacgaca atgcccaaac caatctcggc gtgttgtatc
    38941 acaacggtcg aggcgtcacg aaggattata gggaggctgc caactggttt cgtaaagctg
    39001 ctgaccaggg cgaggccaac gcccagagca atctcgcgtt cctctatgat ctaggcaatc
    39061 gtggtgaacc acaagactac gggaaggccg cgttctggta tcgaaaagca gctgaccaag
    39121 gtcaccccaa tgctcagaca aacctcgcct ttctatatga gaccggtcac ggtgtgccac
    39181 aagattaccg gcaggctgaa atgtggtatc gccgagccgc caatcaaggg tacgccaatg
    39241 cccaggcgaa cctcgcctcg ctgcacgctc agggcttagg cctaccacaa gaccttaagc
    39301 tggctttcga ctgggctcgc aaagcagccg aacaaggcca tcccctcgct caaggacaat
    39361 tgggctacat gtatcacatg ggcctgggtg tcacgcagga ctatcagagg gccatttatt
    39421 ggtaccgcaa agcggccgca cagggagatc ccgcatcaca acacaatttg aagatgctcc
    39481 ctcccgagat acttcgcaag tacgatacga ccattggcat ggaggagctc acaaagactg
    39541 ttgttgcgat tgcggcgacg gtagcgtttg tgtcgatatt cgatttcata tctcggggta
    39601 gcggaacagg aacaactcga tcgaatgacg ggttaagaaa tactccggga ctcgacctag
    39661 ttttagaccc ctatgatggc agacataaga cgcgttacga cctgcagact gacaaaatga
    39721 atcgagacta actggtttct tctgaagatg ctttggggac ggaacaaacg tcagtgcgat
    39781 acagaagtga ggagtggcgt tgccactgta gtccgccact aagattgaaa atggccccgc
    39841 ttctttattg tccatctgcc ttggccagcc agttacgcaa tacattttaa ggacggagga
    39901 agatgtcaat gtcaaactgg gttactcgtg taacgacggt actgacactg tcaatgttgc
    39961 tcaccggttg tgcgagcttt gtcgtgaagg tgagagatga tgtggccggg ctaaacaggc
    40021 taaaagtcag gagagcgcaa gaagccagtt tggcggtcat tgccaagcga gaactaggcg
    40081 cattgaaggc acagcagccc ctaaaatcgg gggaactcct gccacctctt ttcgctcagg
    40141 cacaacttca agatgcctgg aaagggagtg cctgcagcaa tcaaatgggg attcacatga
    40201 atctcactac ggtgggacgt ggaccgatgg gagaaggcgc gagctcgaca ctggccaagg
    40261 tgcgaggtgc atttacagca gctgatcagt ccaaacagcg aacggcacgc gtgattgaaa
    40321 caggcctgga aggaacattc gatcgtacta cgggatttct cagtttgcat gcaattccca
    40381 agtcgacgga actgacgcca gacgaattgg aagcggaact ggcaattcga gcccatgtgg
    40441 agaatcaaat cttcgccatt gatcaacagc taaactcaaa tccgagtgcg agtgaaaaat
    40501 acaaactgat ggacgagagg gagaagctgc aaagtgatga aaagaatcgt ctaaagaaat
    40561 tcaccgaact tgctgtagcc aaagccgcag cagcaaaagc agagttagtt ccgttcattg
    40621 tcgagctcgc tcgagacaat gatggcaaag ggtggagagg gactattgac ggcactgctt
    40681 ttcaggactg cgaggccgcg ttagtgagcg accatggact caataccgag aaacttcctc
    40741 ccatcacaag ccaaatgatt ttgaagaaag ccagaagtca gggggatcta aatagttatt
    40801 ggttaaattt ggccgcgagg gacgccagag agcaagactt cttctttctt gcccagttat
    40861 atgaacgcca tggcgtagac tcaccagaga attatgtgcg cgcctttgat tactataaaa
    40921 cgatcaatga taagaaagag gatgcacgcg tgcagtcggc cctcagccgc atgtatgcga
    40981 atggtctcgg gacaccgctt aatatacctg aagcagcacg actgaatgct ttggctaccg
    41041 aaacacacaa aaaagctctg gctgtttgca aatcccccga gacgattgcg ttaattagcg
    41101 agatggcaga gcgggctcga cgaaaaggca agttattcga actcattgcg agttgggcga
    41161 caggcatgga aatagacacc ttgaccactc gagtaatcaa gcgaatggtc gggaatgtca
    41221 tttcgataga tcaaccgttt agctgtgaga tttgggcccg aaggatagat ccccgcatta
    41281 atgccagctt aacgccggac tattaccgtg ggtatgacca gtacggcaat tactatgagg
    41341 aagacaatgg cttcgagaaa ggattgaagg atgctgtggg cgctgccgtg cagaagctgg
    41401 cccaactaac gcctgttttt actcgggtca ctgttgaacc tcaagggcag cagcgctacc
    41461 aactaatttg gagtgatgga ctacacaggg aatctcaaat gctggatttg aactagttct
    41521 cttcaaatct ctcccgagta ttgatgtgaa cattaagggg ctagattgtg gctgctggag
    41581 tcacgaagga ggcggagcgc ggtcgaagct gactgtctgg tgcggttgtg ggctgcggcc
    41641 tgcagggggc gtcgatcttg agcagcgcta gctcctagat gtttcagaga ctactggttt
    41701 ggcaagcctg tggggactat cggttcagcc ggagttgatc tctccgtata cgacgggata
    41761 gaaggggatc atcgagtagt tcttctgcag tctcaaagaa gaatgtgttt ggcagcacgt
    41821 gttctggacg ctcgaaaacg tgcgatgcgt caagcgagtg tggatgcagt ggtacaacca
    41881 ggagcgccgg catcggatat tggggtatcg gagccccatc cattatcggc acaacagtta
    41941 gcccaggtgg cttgaattca agagagcgtt acggaggctc gaagtgttaa tttggaacgg
    42001 aaagattcgc ggtcgtatcg agaggcctac tcgtgatctc attctaatca caaattcaat
    42061 gaatatgagg gcgtttaaac gatctgacag aaaaatcatc tccattctta ttttgttcgt
    42121 cgcttatttc ctgacggggt gcaagtttac ggaacattcc ctatatagcc cggacgtagg
    42181 ggaattttcc cctagcctga taggatcata ttcaatgaaa cgaggcgcca aagagaccac
    42241 cgagctagtt gtgcttcgaa agtctccaac aaaaccgaac gcttatgaat ttatcgaggg
    42301 caatacgact cgacgtgatc tacgcttgat aaaacttggc aactggcttt tcgtagaagc
    42361 agactgtggg agctactact gtgcttggag gtacacgaac gatgacggag taattacact
    42421 gtatagcgtt gatggctcca gagaggctga ctctcgaacg atggacttct tgcgcagctt
    42481 gaaaattgaa gggcacagca caacggctga agagcttacg aaggatttca acagcctatg
    42541 gacgctaggt ggctctgacc tccgagacag cccttcgccc tcacatcaac catcctcgcc
    42601 ccctccttca aaagtggacg ttccttcggc tgcagcaggg agtgacatgc ctcaggttat
    42661 aggggagatc cccacccctc agccggctgc acattcaact ctggaaactc gtgaccttca
    42721 aaaggcattt cgcaaatatg gggatacctt caacagggtg tacgcagaat tgcgtaaaat
    42781 taactgactt gcgtatcaaa tagaattgtt agcctaggga agatgccatg tcatacaaat
    42841 ctctccatct aatgatgatc ttactattgg tcctcggcgg gtgccaccca atggttagta
    42901 ctcagccact ttctgaccca atgacggagg agcccgataa atcgttatac ggtcactggg
    42961 tatcaattac cgattccgga actcagtatc acgtttatgt aggtgaaagc ggtaaaaacg
    43021 caggagatgt ttcgaaacct ttcatggaat gcctgctgtt ttcatggaaa tctgacggaa
    43081 gtactaaaaa aataaacccg atgtattctg ggtacctcac tgtaagtcgg attggtagct
    43141 cttcgtacat caatgtttat gcgaccccag acatggaggg gaagagaata gaagcacata
    43201 ctagtagtga caattggaga tatgaaacat ggttaagaaa ccctgacaaa ctggtgggga
    43261 tattccggta tgctgttcag gaaaatcgag tcactttttg ggaggcctct aatagcaaac
    43321 cgcttcaaca aaatctcctc gagagggcag aactcattgc agtgccagga aacaaacttt
    43381 tctcagatat caaaccacca gacacaccag taactttcga tagccttttt cggtatcttc
    43441 gtaaaaatgg gggagagaca ctcttcggga gtcctactac cttcactaaa gtgccgtaac
    43501 tttctgggag gctaatacct gcaatgattt cgggttatac gtgagaggcg cactttagct
    43561 ggctgtacgt aacgtctctc ctcaaaacgg tggcgatcta agcgagagtg ggtcccaggt
    43621 gggagctgac aggaacttaa aaggatgctt atcagtaccg cgtgatcgca tattccgccc
    43681 cttcctccag ccggtcaatc gaatgaaagt acttttgagt cgtgcggatg tctttgtgat
    43741 ccagcatggc ttggactttg aacggcgatt cagtccgcgc agcctccgtc gcggcagtat
    43801 ggcggagact atgcggggtc actcgtcgct gccggtgccg gcgactgcct gagggtgccg
    43861 cttcaccagg tcgcgcggct tcccgcagtc cgcatctggt catggcttcg gtaattcgta
    43921 gccggatagc gcgggtcgta agtcgtaagc gtattcttcg ccaccgttga gcccaccacc
    43981 tttagtccac agctcagcat gttcacagag agcttgggct gcggacacag catttcacct
    44041 ctaaacccag cagttgttta tcaacgcgat atcgtaacgt ccatgatctc agccgagcag
    44101 aggttaatta tggggatatt cactcgatcg gccctctcaa tatgtatgat agcgatggta
    44161 gtgggctgtt tgcgcatcgg tgatagtaac tccaaacctg tgggcaaggg gaaccggaat
    44221 cggtcaagag cgctgccgaa atatatcgtt agcgatgagg tcagaggata caaaggatga
    44281 tctgccttgc tacggatgaa cagaaacgtc taaagctgaa agagctgagt gaaccaaggc
    44341 gacagttgga ttggcggtga tcgacgagcg cggcaagcgt acctgatcac catgagaaca
    44401 cccgtttgtt ggttccccta cccgactttg aggacatcaa ttggcagaaa cttgcggtca
    44461 gtgccggcca tccgcttccc aatgccgtaa cagtatttca ttttttcagc gatagctata
    44521 ggatgccctg acccgcaact atttaattct ttattcggga gggacgttca ccgacgggat
    44581 tgagaggctc tccatagact ggatgttgaa gtattggcaa tgacctgtga ttgaagtcaa
    44641 tcatttggac ttcgctttgg agaaaggcac cgcttgtggc aaggacacgc atgcgcgatt
    44701 tggtgattat ccttcccgga atcactggaa gcgttctcgc atacagtcct ggcgaaaaca
    44761 ccccagttga tgtctgggcg ccatcggggg aagcgttgtg gcaaggcctc acgagtctcg
    44821 gtgacagtgt ccaacgcttg gaagtgccga cacacaatcc tcgacatgca ccgccggaaa
    44881 caggtttcat accaacccgc gtcgtcaagg attttcacgg agtgtttgga cttggcaaaa
    44941 tcgacggata tacggcactg acgaatctaa tcagcgatca cttcattgtg acgcctgggc
    45001 gtcctgacaa cgaagtaccc gctaacttct tcgagttccc ttacgactgg agattgagta
    45061 accggcattc ggccacccgc ctgcgagact tcgtcaatac tcgcctcact cgatggcgca
    45121 gtggagcgcc agacgcgaag atcattctcc tcgcacatag catgggtggg ctcctcgcca
    45181 gatactatct cgaggtgcta gacggatggc gcgactgtcg cgcgctggtg acgttcggca
    45241 cgccgtaccg cggagccgtt aacgcgatta attttattgc gaacggatac aaggcgtcat
    45301 tcctcgacct gacagccgtc cttagatcct gtccagccgt ctacgaactg cttccgattt
    45361 atcgggctgt gagcgaaaat gggacgtggc gtcgcgtcgc cgaaggcacg ctaccgaatg
    45421 cagtgaacga ctacgtgacg gctgcctcgg aattccacca ctctatcaag caagccgttg
    45481 acaaccattt gaacgacgcc gagtacctga agaaccgcta tcgtattttc ccgttcgtgg
    45541 gcgtttcgca accgacgttg cagtcggcga tcctcgacgg aggcacggta cgtgccagca
    45601 ctggttgccc tgactggatt gacaactcgc tcgagggcgg tgatggaacg gtgccgcgcg
    45661 tctcagccac tcccatcgag ctttcagacg actaccgcga aaccttcttc gccgaacgcc
    45721 acgcttcgct ccagaacaac gggtatgccc tcgacgacgt gcgtgagcgg atcaagcagc
    45781 ttcaatcaag cgggactcgc gagatcatgt cggagtggcg agcgggcacg cgtcccgcga
    45841 ttagcctcgg actcgatgac ctttacctgc cgggagagcc agtcctgctg tatgcatcag
    45901 tgataggagg acgtattcct ggacttgtcg ctaggattga atcctccgta gcggaaacta
    45961 ccgaaaggca gtttgtcgcg accgattcag gatggagatt gcgactcgaa gggctttcgc
    46021 ccggacttta tcgtgtgcga gtgagatcga ccatgggagg cgatacacag ccgccgccag
    46081 tcagagatgt gttcgagatc gcaggcgcat aatttcgagg aggttcacga tgccctgggc
    46141 agcttgggcc aagatcaatg acaaggggct agcgcagtat gtcctgcctc agatcgccaa
    46201 ccgggtgcgt ttatcgatca cacgtgatga tgcgcttacc aagccgggag gccgcaggga
    46261 cgtcacagaa gccatcttta acgctctatg cacgtttgac atccgttacg cacgcccact
    46321 gtacaatcct attcaggagc agcagacgat tcgggccccg gagacgatcc ttgacggatc
    46381 gggagacgga acttgtctcg acctggcgct cctatttgcc ggagtggcac tcggtaatga
    46441 attgctgccg ctcgtcgtag tgctcgacgg ccatgcagtg gtggcggtct ctatggaatt
    46501 cggccggcgc gacgcagacc acctcagacg tccaacagat gacggtgagt gggcaagcac
    46561 aggaattctg agccatgtga caacgctccg cgcgctcacc gaccagaacc gctaccttat
    46621 tgtcgagtgc accggtttcg cgaaacgtga cgctataccc gctgatgtcc cggaaggcca
    46681 gggtcgcgtc gacggtcacc tatcctttgc acgtgcgatc aaagcagggc gcgagcaact
    46741 cgagcgcgct gatcgcccgc tccaattcgc cgttgacgtc gcatatctgc aagacatcgg
    46801 caagcagtca accctcgacc cggtcggccg gagcctcgag cgtgtcaagc ctcagttgaa
    46861 gcgccgcctt gcgcgcatct tcgaaaccca tcagctcttt ggtgggcgca gtgaggagct
    46921 cgcgcggctc gacgcctttt tggctggtcc ttctggctac atggttatca cgggaggatc
    46981 gggatgcggc aagacctcat tgatagcgaa ctggatccga caactcgaaa cgcgtagcac
    47041 gcatgtcgcg tatcacttca tcagcctgca gcacgatacg gcaggacacg acgaaacgct
    47101 actttcccta gtgcaacagc tgtcctactg gaacagcctg ccgactttgg ccacatcagg
    47161 tgcagaggtg gaatccagtt tcatcgagtt gatcaccgag tcgaccgcgc ccgtcgtcgt
    47221 cgtcattgac gcactcgatg aagccaaggg ctggcaagtc ggacggcagt tgtttcccgc
    47281 tcgactggca cccaatattc acgtcgttct gtcggcaaga gtcgttgcgg ggaaagactg
    47341 gatcacacat ctcgatcttc aacgcccgga gcacatagaa ctcggtccat tgtcgcaggc
    47401 agaggtagag gaggtgttgc gcgccgctaa agtcccggcg tgggtactcg acaagaatgc
    47461 gctcacccta ctcacggaca aaatagccgg tgatccgttt taccttcgcg ttctagtcga
    47521 ggatctgttg cggggtgata ttgcgacacc actggctctc gcgaagcaac ccaggggcat
    47581 caatgagtat ttgcgccagt ggtgggatga gattcggcca actgcttcgg cacaggcagt
    47641 ccaggatttg ctgggctacc tgttggtcgc gcgagggccg atctcggcaa gcgagctcaa
    47701 agaagtcagc cccgacgatg cgctttccgg gttttccttc gacgcggccc tcgagaacat
    47761 tcgtcggttc gttgtcggcg ataccaatcg tggcttctcc ttgagtcact ggcgctttca
    47821 ggactatctc acccaagaag tgctaacaga cgctgaccag cgtccctacc gcaatcggct
    47881 agaagactgg tgtgatcagt ggcgaacaaa caaaggcccg tatgcgttgt cgtacgctgt
    47941 cgctcgccgc ctggacgtgc tggccgccgc tccgcagtcg aagcggtatg acgaaattgg
    48001 gaaagtcgtg actctcattt gcgatgagga gtatcaaaaa cgacgggtgg aagccacaga
    48061 cgctctcgca tcgttgaaac tcgacgtgcc acgcgtcata caggagatcg cacgttcggc
    48121 agacgtatcg gtcataccac aactggcacg cgtcgccatg gaacatgaac acattgaaca
    48181 cacgtggttg ctgccagaat ccatgctggc ccccgcgagt cagggatatg tcgaagaagc
    48241 tgaacggcga ctagctctct tccaagccga ggatcattgg cgcgatgccg ccaggcttct
    48301 cattgcatgg ttggcggcac cgtacgacgc cggcgctgcg cgaagggcac tgcgtgcgtc
    48361 tggagaaaag tggacgggtg taccgccact tccgatgtta cgcgatcgcg tactagccac
    48421 gattggtgcc ggagatccac cgattctcac gctgccgtat tatcctaatg ttctacctga
    48481 gccgcgcagc gaaggcgaga tgcagcagat cgtggcagag ctgggagccg atcaatcggc
    48541 gacgatgaga atctctgggg tcgagccggt gcgcgtgcca ggacgccagt cgttgcgcgc
    48601 gcgcggaacg ggcaaggaaa cgccgaccta tattgcggaa ggcgatgcgc cacatctggt
    48661 cgcctttgcg attccagcaa cgaaccgtga ggccggcaca aagttgattc gccaatatat
    48721 cggcattcac gcggcgaacc cgtacgccga ttatcggaac cgatcgctgt ggggaattct
    48781 tggcgcagtc ctgtgccacc ccgagaccga ctgggccaga gatacgacga aattgctcgt
    48841 cacgggggca ttcgcgccaa gtcctatccg ctttcgcgag tcgttccgta tcgccgtgct
    48901 gtgccgactt gaccaggcag gcgtcgcaga tggtcgcgca cagttcgatc gaattgtgag
    48961 cgagacacgc gccggagctg cccgtattcg cgaaacgcga tgggaatctg attcttgggg
    49021 caattacagc aggcgttttt cggctttggc cgaagccgcg gcggtcgccc tcaaagaccg
    49081 cacactggca gaagccttcc tgaacgaggc cgctatgctt ccggtcggat tcgctggcta
    49141 ccaagcccct acgagcctga cactcgcgga agcgaacaaa atcatccggc cgaatgatac
    49201 cgtttcgagg tcggccgctc ttactcaggc gagaaattcg gcacacaacg ttcaagaccc
    49261 gggtttctgt gcgcgtacga ccgcgcgggt caacgcgatg cgagaggtgt ggtgggccca
    49321 accgttagag aacttgaccg gtattctcga cgagtttatt cgcgatccat tcgagcgtcg
    49381 attcgccccg atccatcgaa tcgccgaaga ttttgaagca cgcccgaaga ccgaagaacg
    49441 cattgcactc gacgaactca agaacgcccg tacgctgacg gacctcgccc aaaacgtctt
    49501 tcacctcccc gtttccgctt tcgaacgact caatcccggt gtccctcagg agatcgcggc
    49561 ggtagaccag atcgcagtcc ctgatcccaa gtttgccccg ctgctcgcgg cgcgattctc
    49621 agctgaacta gtaagtcgca ctgatctgtc tgacgatgat cgggtttctt atctggcccg
    49681 cctcgttccc attgcacttc caaatgcgac aacactcgac gtcgtcctcg cgcggcttac
    49741 tctcgcgatg acaacagctg ctcccttgat gctcagcgag ctccttgctg tggcgccggc
    49801 gttggaggat tatccaaagt gatacgcaac ggtctgtctg acggcccatg atcttaatcg
    49861 catcttagct cattgccacg ccggtccgag ggtagtcatg tgcggacatt ttctgactct
    49921 cagtttgtaa ttgtgtggac cacccgcctc tacgtaatac gtgtattttt gtgatgattt
    49981 gtctacgtag tgacaataat tatcttgact cgtttacacg cttccggcac tgtgaccacc
    50041 tctccagaga ttattgggag attgtggcat gccagaaatg gctgagcgtg gagatcaggg
    50101 caagcaaggg tcaggcggag aactgcaagg ccacaccatg gccctcagat agacctcgaa
    50161 aatctgtgcg ctcatgatcc gcatacccac gcaaacgctg agacgtggag agcacgtttc
    50221 cttgcctagt gcaacggctc cgactcaaaa gacggcgcat caaaataata acgtccctct
    50281 tcacgaacaa ctcgatcgag aaactcggtc atccccttcg caatgacacg cgcactgtcg
    50341 gcttgaattg cggctccctc gaggcggccg accagaaccc gcccggctcg cctggccctg
    50401 tccttgccac ggaccgtggt ataggccacg gcacgaccat cggctaaatc catgaaatgc
    50461 acagtctcag atgagtcagc tgcgccgata gccctgccgg tgatctcggt agctggcctg
    50521 agcgtccagg gatgtggcgt ctgctcgtaa aacacgtggc cctccgcaaa gcgatccagc
    50581 atctgcacca attctttagg gatgccaacg cggagcatat tgctgaggcg gcaatcctga
    50641 ggggcaatca ccagcggttt ggaaatggcg tccctcgccc cccttcgtgt gagcgtgttc
    50701 agcagctggg tgaacagctc tggttccgca accgcccacc cgatctccgg ttccagccga
    50761 ccagtgtcta cgtgttgcct gacacccagg aagccagcaa gaatctccaa ttctatcgat
    50821 tgatcgatgg aagccgattt gaaggtgaac ggggctcttg acagacccgt gggaatcatc
    50881 ccggccttaa gcccatcctg gacggtgagt tgtgtgcgtg gaatgtcgag gatcggattt
    50941 ctcacggtgg gtgccttcgt gaccgactgg atgagatagg ggaagagatc ggccagccag
    51001 ccggtgatca gatctccccc atagatctcc ttgggactat agatgtgttt ccagaaccgt
    51061 tgcgacgggt tgccctgcgc cgtcgcgaca aacccgtcac aaatcggttt gagccgatcg
    51121 gtccaccagt caagctgaaa tgtcgccatc acgtcgacgc gttcacggat tctcacccaa
    51181 tcagcaacag agcccgtcag cgtaatcgaa ggaatcccgc aaataaactt cagttcgtaa
    51241 tcaaaatact gctggaacgc ctccatcatg acgacctgac ttgctgtgcg tatcacaggc
    51301 gaggtcgtgc tgaaatcgca cagcatgagc tggtacaaat catcctgaac atgctgccga
    51361 atcccgtcac tccattgctg gacaaccccg gcccaatgct gagatgcgtg cggatctatg
    51421 gtctccgcat acaagatcat tttccccttg tgtcgaacga ggctcgaccg aagcgcctca
    51481 gcatgattca tgatgtgatg agcaaatccc tgcgcaagcg tcatccacac ggcatcaggc
    51541 gtcaatacga gcggccgatg ctggctaaac gccacataca ccgcatccac gaagccatgc
    51601 gcggcggctc ccgttgccga aataagctct gacgactcat cgtgactaca ggcaaggaat
    51661 ggtctgccga catgttgcct cacgcgggcc tgtgccgggc tgagtgtgca ggggtccaga
    51721 cagggctcta cctcatcgac acggaaacaa atttctgtgg ctgtgtgatg aaggatcggc
    51781 gcgtggtcgt tgacagatga aggtatctct ttcaatgagc acctcccagt atcaaaatgt
    51841 ggatcggcag cgtgcgcagc cctaacctac caaccaatcc gatctaattg cgcctgtgac
    51901 atattccgcc cactcctcca atcgacaatc gcgtgaacgg aatcctgtaa tcgttgcaca
    51961 tcaacgacgt aaggatggtt tgatctaaca tggcaaagtg agccaatctt tggccaccag
    52021 ggcattagcg cgaagatact gcattgacct tttagctcag atctcttaaa tttccagcca
    52081 atatggtatt cgcgctaatt tgacaaggat gctatcgacg atgaagctgc acgtagaaaa
    52141 atgcataggc atctccacac ctattgcagc ccattaagta atgtgacctg agtcgaagtg
    52201 ttccccacac ttctgagttc aagccatgtg tcaccatgcg atggtttcac cagtgtgacg
    52261 gcggatcatg cacacgaata tctcgctctt gcttacgttg ttggatcttg ctcctcagtt
    52321 gcaatgcgat cttggcaccc gctggccgga agtctgtaag agtctactga cactcgcagg
    52381 gcgtcttgca aacgaagggg acactctgtc aattcgccgt gatctggata gtctactaga
    52441 tgagctgttc gctatcctac ccgagacatc cagcgccact ctccgctcga agttgcaaga
    52501 cttgcataga gaggcagcgg accagccctc gagactacgt gcgtttggcg agcaagaccg
    52561 actaactggc gacagggagc ctgagtccaa tgatgcgatc atcgtacctg tgttttatgg
    52621 tacggaccgc aaacgttctg acactcccct cagtccatat tgtggtcgat ggggggagca
    52681 ggattttgga gtagcagaag tgagtgttcc tattagcaca aaatttcgtg cgatcggcga
    52741 actatccagt ccgaaatggt ggcgccttga gttcaatccc gatactaaga agcacgttgt
    52801 gttgctgaat gtgcgtcaac tcgcgcggga aatcttcatc gctgagctac gcgacagctt
    52861 ggcaactgct gatcggaggg atgcgttaat ttttgtgcac ggttataacg tcagtttcga
    52921 cgacgcagcg cgacgtgccg cgcagcttgc agtggacctc aagtttccag gacgaacgct
    52981 cctttacagt tggagttcat ccggattacc tcaaaattat attatggatg aagaaacgat
    53041 tgagtggtcc acaccacatt ttgaagagtt cttgcagctc gcggtgacag aggtaggagc
    53101 tgattccatt catttactcg cacatagcat gggaaatcga gccttggtgg atgccctgag
    53161 tcgattcaac gccggcgcat taccccccgg gtctgcgcac cttcaacaag tcatttttgc
    53221 cgccccggat gtcaatgcgg gcgtgtttcg tcaacatgca gctcgctttc aacatcacgc
    53281 caaacggttc accttgtatg cctcgtcaac agacctggca ctcaaagtct cgaaattgat
    53341 acatggccac cctcgagcag gcgatgtcgg ggataccccc ctcattgttg acggcataga
    53401 tacgattgat gctactacgg ttgatacgag cctcctaggc cttaagcatt cttattttgg
    53461 cgacaaacgg tcaattctca acgatatctt tgggcttgtt cagaatgggc atgaaccaaa
    53521 gaaacgcttt gatctcacag ctgcgatttc ctcagccggc gcttactggc tgtacaggcc
    53581 gtagcgttca agctcatatt cccacataag cgaatgccgc ccaatgctcc ggagatgcaa
    53641 acggcgtatc ggttggatcc cgtgagagaa ggtcccgcca taaattcgac agcatcattt
    53701 cagattcgtc atcatgattt ccaagcgccc tacgaagact gctaacggtt cctttcaaag
    53761 tgcccaccgt tgcctcgcga agccaactct gggcagcgtc gacagcagcg gccccactac
    53821 gccgattcac aaacagttcc tggtacagtt gctgggaaaa caaggcggcc acacggtcat
    53881 ctacccgcca catcgtacac gcgatccgcg gcacaccagc cagcatgagt cctaacggaa
    53941 gacccaagac ttcgtcaggt acctcccgaa attcaggatt agcggtttgg caggccgaga
    54001 gcaccatgag ttccgcagca gaaaaatgaa tatgcccacg aagcagctcg ccgagagtca
    54061 agatcccgtc cgctaatacg agtcccgacg cttccggttc cgaaggtcga aaaagtccat
    54121 gacaggcgaa atgcaggtga gtcgaaacat gggcggcctc tgcgaccgca cgtttagtcg
    54181 cacatttccc aacaaacacc gtggttcgcg tccagcgtcc cggctcattc accgctcctt
    54241 gtacctcagc tctcgcaaaa ggtaacgaca tggtcggatc cgccacacca cagagaacgt
    54301 atccgcgagc tggtcgattc gcggttaggc tgagggagtg cgctaacgca cgcgcagatg
    54361 gagcatacgc aaaagccggt cctcttccgg tggcagcggc atgaagcggg aggagcccta
    54421 gtcgccccaa agggatgagc gtggcttgcg cataaccata ctcgcgcagc cgatcggcta
    54481 taggctcgac aaccgtgtcg cgcaatacgg acagcgcggt ggggagcctg gcagcaagag
    54541 cacaatggtc atgccccatg gctccgtcaa gcattccatc ctcaagcagt cccgtgacca
    54601 catcgcttgt aatatcttca agtagaatac ccgtcattgc gccactagag tgaacaatca
    54661 atgcggcacc accccaagcg gttgcaagca catatacaat cggctgtcca aaccgcttcg
    54721 cgagcgcggc aagatctgac gactggagtc gatctggtag actgaccaga ttctcagatc
    54781 gtactctggc ggtcgtttcc cgaagccata cgcgcgcctt cctgaggtcc gcgaccttct
    54841 gcaactccag attgataaat tcctgtactt gcgcagaacc ttgggttatc cgcactttta
    54901 ttgccccggc atcaaccccg aagtggtccg caaggcgccc tgctatgtct gccattacac
    54961 gctcaggatt cttagcccgc agacctcgaa tgtcctcctc aagagtgcgg acacgctgca
    55021 atgcattcct aacttcatcg cgagactctg gagacagact agctgttaac atttctgatg
    55081 ctaccatcag atctacgctt ccgcgtgctc gccctgcttc ggccaatcgt gctgcttctt
    55141 cgtactgtcc gagtcgcacc gcagcataac aggcgaatag agcaatacca cgcatatcag
    55201 cgagcttctt ttgtctctct tccgacgtga cagcgtcatc gagtagctgt tcactcagtg
    55261 caagtgcgtc tccattggct cgaagagatc cctcccaaag ggctatctga tgacacatat
    55321 cgcccatcgt acggctaatg tctctataga gacgtgggtt tgatgacggg tctattgctg
    55381 tcagtgcaga cacgaaacac tcgcaggatt gctcgatatc acgtcgctct cgtgaaagat
    55441 ggtatcgctt agccagcaga tagcccagcc cggcgcgcca gttcgcccat tcattcggca
    55501 tccttttgat gtctatgcct gagagggttg agcggtgttt gagaatggcc gcatcgagag
    55561 aagccaaagc gcttctgact ggaaggcgca cgatctcatt caaatcggcg taaagtgctg
    55621 attgttggcc tgagaagtgt ccccattcac cagtacggat ggccgcgcct ggttcctctt
    55681 gcacgatctc atcagcctct tgttggaacg ccatggcaag attattcgca tgggcctgcg
    55741 actccgcacc gctccactcc cgaagggcta acgcaagtcc acgaagcgta cgtgctcgtc
    55801 ccgcagggtc cgactcgcgc gtccgcacga cgagtgcctt ttgtagcgcg gtcactgccc
    55861 gatcgacatt ttgcgttcgg agccccacgc gccgtttgag gtatagcccg gcgagattat
    55921 gctgcgcccg accaaagcgt gatgcgtcac ttgaactgag agaacctaac acctcgattt
    55981 gttccatctg tagggcaatg gcatgctcaa tatttgcatc ctgatcacca acaggtgcgg
    56041 tagaaacgag ctggctataa cagccaagtg catgggcgag cgtgtcggag gggccggcac
    56101 cgcgatggcg cgttaccaat cgttcaatca tctcaaaggc atagaccaat gggtcggact
    56161 cgacgcctgg attcatgccc aaacaattag cataaccgat cactgcggcg tcccatactg
    56221 cagactcaga ttgactgaca gttagaaggt ctgtgtaagt ggcacgtgct cgggcgacgt
    56281 tctctgcgtg gtttccctcc ctgcgctcta ataggagatg cccccaccgc aactgcaagt
    56341 cgcaccacag ctcagacccc cgttcgtcgg tgatcaaagc caacgcctgt tcgcatgcag
    56401 cgaaatcctc ggcggtagcc tcatgcggtg gcacttgtcg taaccgcata gctataactt
    56461 gagctttcgt cacgtcttct cgtgtgcgcc catatggaat tcgcattcac cgtggagggg
    56521 cgatctattc aagactgaaa atttccggct gcccaccttt cactttagac gatacctgcc
    56581 ttgacttagg actctcatta acgacgacgg ggatcgaccg gcgtagaccc gcctggtacc
    56641 atgggggaca caccgcaagg cccctcgccg atacaatccg gggcgattcc cccaggtggt
    56701 ctcacctgcg tccgattctc ttctagttga attttgttac agggacaagg ccggtcctgg
    56761 aaacaacgca ttgctccgca cttcggatca ttagtactga tccgttgcaa gtctccagct
    56821 tgcacagcgc aagacaagac tgtggaaatt ggcgaagtag tctgttctgg atcaacaagc
    56881 atgatattgc tgttaccgga aagctctcca tgtgttgggg cgtgagcacc ggaaaatgta
    56941 tgcgtagttc cgcccgagtc tccgctggtg cattgataga gtgcctgaac gcgtctcgcc
    57001 gcatttgacc ggttcccaca gccactagtc cctccaagtc catcatccac tgtacgtcct
    57061 tgcgatggcg cggatgttgc cgcagaagcc ttcgctgctg cctctgcttt tttcttggct
    57121 tcttcttttt gtttcctggc ttcctccgct tttttggcag cctcttcggc ttttttgcgt
    57181 gcttcttcgg ccttctttgc tgcttcttcc gaaagcgtct tagccgcttc tgctcctcgt
    57241 gccctggctt ctgcctccgc tttggcctcc aactcagctt tcaaggcctc tgtcaatttc
    57301 ttggttgcgt cgagcgactg ctcgtcgaga atggcctgct gctggctcct ctcggcactc
    57361 aacgcgtcca tgtattgcat cgtccgatct aattcgtcgg ttaggacctc cagtggctcc
    57421 tgatccagaa ttgcatactt tcggattgcg tcaaaaagcg cccctatccg ctccttttcc
    57481 tctttcgtaa gatacaaccc taaagtaact cctgaacctt ccccttcgct tcgagcgcat
    57541 tgtttgtcat gaattggcac atctgatgag gtaatcctgc caagacctgc tgcggggtta
    57601 aggttgcacc agctagggct cgttgcatgc aattaagttg gccacgtacg ccgacattag
    57661 aagccgcttc aaggatacac ctcgcgccat ttcccgtggc ccctcctgta gcgcttccac
    57721 caccgaccgc tgtcccgcca gcaccttgct gtccaccctg tgggcactct aagccattcc
    57781 cagtcctctc agaacgttcg accatttcca ggagcgaaca actcccatct cccacacgat
    57841 gtgtttcgga caataggctc agcatgttat gtgttactct ctggtcattc ttttggacca
    57901 gctgcccacg tagaagatca tatagccata ctgcctggct gtcggcttag cggagggcat
    57961 agacgatctg ctcttcggag aatgtcgacc gcctcatcga tccatcactt tcgtcgagga
    58021 ccgccgaagc cgtcattcta cgccagtttt aaatcgccgt cgttttcgga ggagacgtca
    58081 ctccgggttt gcgaatctgg gcgtgacgcg gcggcacctg ttggcagaag tctagcagct
    58141 agccgtgcaa cgacgttacg ggcagcccga cagatgggaa catacttttg ggcaaaacaa
    58201 ttccagatga gtacacatgt tgatcccttc aggacaaagt tctgcgtgca aatgtgaatc
    58261 aatttaatca gaccttcttt agcttgtgtt tgtgagaatc tttgtacgca ccacacagct
    58321 ttgtaccgca gccagtaatg ctggaccatc tattccttga ccaatgaaaa cgagttggca
    58381 atcagtgatc ggaccggcat acggttcgaa ggaagctcgg ctaccacaga gctgaaagat
    58441 gaaacgtcgt tcaggggagt cgagagcaat gatgcccttc gcacgaaata tgcccacggg
    58501 aactgcatga tcaagccaag cctgaaacaa ttgagccgat aggaaaccct cgaagtgtag
    58561 aacgtgcgcg gcgaagtcgt ctttggcgag gtgaggacta ttgatgggtt cagggtctga
    58621 gacagaggcc aagctcgact ctccgaaccc gccaaccatc acagcatggg gaatccgccc
    58681 atatgatgcg cggaggaccc ttgtacgcgg cttaatcagc gacattctcc gttccaggag
    58741 gctgagatgt tcctcgccta ctaggtctgt cttgttgagc actattacat cgccatgaac
    58801 gatctgagcc atcgccgcat cacttttgaa gatatcgagc gcgaaattgg cggagtcaac
    58861 cacggtgatg actgcgtcta gcgaggttcg cgaccgaagc ccggtttgaa gaaaggtcaa
    58921 ccctatcgga agcggatcgg cgagtcccgt cgtttcgatg atgaggtggt cgatcggagg
    58981 ttctaccgtt agcatgcgtt ccgcagcggc cagcaaatcg ccattaattg tgcaacagac
    59041 gcatccatta gtaagctgga ccacttcgtc cgccatcgaa cctaccagcg ctgcatcaat
    59101 gttgatagcg ccgaaatcat tgacgagagc agcgacgcga cccagtttac catccgtaag
    59161 aagatgattg agtagcgtgg ttttaccgct tcctaggaag ccggtcacaa tcataactgg
    59221 tacccgtccg agtgcatcgc gcatcaggat gtcctcacca tggtgtagcc accgacgagt
    59281 tgtccgttcg cgtcgtagtt cgcaatccgc aatacatggg cttcaccgtc gccgggcgct
    59341 ccgtgcgtct cggcattggg gtgcaggcgt ttgactgaac gaaaaagttc ggctaagggc
    59401 accttcccga tagcgcgtcc tgcgtcagtc aggcttcgga ttgcgacgac gggatcgatc
    59461 actcggactt gttcacggcg aaggaatgga ggaggtgccg gagcgccgcc atgcgataaa
    59521 ggatgcatcg gagccaacat cccagcgaga agacgcctag cggaaggaac gacaatctca
    59581 ttctcagccg tcagctcgac gagcggaaat gtattgagcg ttggtgcgac cctgagccca
    59641 ggagccacag tcatggcagc cattccccct tccttactac ccagctgaat cagctgtaga
    59701 tgccctcccc gtatgcgaga aagattaagc ccagtgagaa gtttcatcga ggttgtgacc
    59761 gatataacgg atatgtttct ctgcgcgatg tggcggttga gccagggctg ccaatcattg
    59821 cccccgagcg gatctccgat accagccacg cgaaccacta ggcattcgtg gccaccgttc
    59881 tcgacaacag gtatccaggc cgtcgggcat ttcacaagtt tgtgcgagcc ctgcatgccg
    59941 cggctggcga gttcgcaccg agctactcca ataagatgag cttgagccga atcgatagag
    60001 agtgacggat tgcaccaata aaactctaca ctgatgccag caaggggtgc gaagcccaag
    60061 ttccacacgt gagcgtaaag agtgtggggc ttaccagcta gaaccgtgcc cccgtgactc
    60121 gcgggaactg ttggcgtagt cgcagggctt cctggggctg tccatatgtc gggactctcc
    60181 cagaacggta cattgatcgg gcgatcgcct cggtcaccaa gtacagagcg aatgaggaga
    60241 tacggataaa agtttcgtgc ccggcctcga tccttttggc gtgacaaaag attgggaagt
    60301 tcatccatca gatgcttctt gcgacgctcg aagtcggcat catcggcctt accctcttgt
    60361 ttcttaaacc agcctggagg catttgatcg ctcatatgaa cttcctctct cggttaatgt
    60421 aacgaattcg caccgatgct tgcggatcgt tgggattaaa gccctggggc tgcggctggc
    60481 caccgttgtc gaattcatca gcgatgttcc acccgtaacc tggctgcacc ggggagggat
    60541 agacatgttg agacacattg gtaggtgttc caccagtaga tggatcgcca tttcccctaa
    60601 aagcatccgg tacagagagg cgccttttgt cgcgaagcca atcccacacg agatagccat
    60661 acccacggtc agaatcgagg ttgaaatttg ttacagtagc tgggtcgagg tcatttcgtg
    60721 tcaatttcgc gatgacataa gcggcatagt ccgttggatc gcccaagtgc tgcccgagag
    60781 ggagaagatt caatgcgagt cggatagtct cttcttcgtt tcgggccttc tcaaactgag
    60841 tcctggcatc gggcgacccc ggtgccgacc caaaaagagc ggtggcgtcc ataccggcaa
    60901 tgaagggacc agcgatgttt tgacgatgtt cgatgttgac cgtattgccc tgattatctt
    60961 tttcaggata gcgccagggg cgcgtgaact cactaggaaa ctcgccattc acgggaggag
    61021 agccaatttt aaagatctcg ctaaacattg ctgtaatagc gtttggcggg tcgagaagaa
    61081 cgtcctttgg ataattggtc ttcggattgc ctgacggatc actcgagacc gtcaccgggg
    61141 taacgccgcc attgaaatcg ccaagagcca ccacagtcgc cgcccaatcg tcggcgacgc
    61201 tcgtgccgag cgtattgaga ccggagttaa tctcgcccgg catgggcata acatatccag
    61261 taagggccag atatgtatgg accgcaagcc agagattgta gagggggagt tcaagatatt
    61321 cataaagggc cacacggagt ggataagtag ctgtgcccgc gatgattccc gctatcaccg
    61381 ctgcgggcca aaccgctacc tcaacgagat aggtcgccca tgcgaacagc gagagaagga
    61441 agtcgaagac gtcgttccac atgctgctgt catctccgac ccctggcccg ggatcggaat
    61501 cgccggaccc tggcggcaaa ggaaaggaag gaataacaaa gacgcccggt tgttccgggc
    61561 ggcggatctt gtagtaatcg gtggttgtcc acttgacata ccggtaaagc caccaatagg
    61621 caccgacgat gtcttccttc tcagcaaaac cctgcgtcgc taatggtacc cgtccatcga
    61681 gggtactgac aattgtcggg caacaagcca ccaaccctgt gcttggggca ctaagcgatg
    61741 gtgtataaac cgccttcaag gtatccgaaa ggaactgcgc gagctcgtct ggcagatcag
    61801 aatcgaaatc ccataccgcg tgccgcccgg tgatgtctgc gctgtggtcc ccatcaggat
    61861 agggtggatt gggttccgcc tgaaagaaat ccacgcggga gctgccatcc ggattaaagg
    61921 ccagccacag gtggagcgct gaattggcca tttgattgta tattggctgt gcaccatgat
    61981 ccgtattgta aacgagagcg tccatatggt tctcaaccaa atggtggcgc tgccagtgca
    62041 agcggtatgg cccgccagct ttctgattaa caaatgcgtg gccagtcacg tcggtcgcca
    62101 gatgagacat ccaccccaat gcaaaggcct ggaaacgtct ccgtagcact gggtctggtt
    62161 cctgctcagc gcgttgccaa agtactgctc cgaatcgata cgtttcccgg taatgaagca
    62221 tgtcgctcca gaaaaaggtc tgctcgtcat atcctgccgg cacaccacta gaaagcagac
    62281 cgaagaaatc atattgttga agaatcagcc tcagaataga gtccttgagg aatgaaaacg
    62341 cctgcccaaa tatgctttcc agcgtatctt ttaaaccgcc cgttagcgca ttgatctcat
    62401 ccgaggcatt atttcctatg ggaccaatcg ccgattcaaa gggccccaga aagttgtcgt
    62461 cccaccatgt gtagatatct cggatctccc cagcaagctt ccagagcatg cttccgaccg
    62521 gcggcttaaa atcaggaaga agaaaaaaaa tgtccggacc tatagatccc agcgcagcat
    62581 aggcaggctc atcgtgcgct atctgcgtca ggcttgcagc atcagggccg ttcgcagcaa
    62641 aaatcggtgc agcggtcgga ttgctggaaa gcgcgtctat tcctttacgt gccacattaa
    62701 tatggatata ccaccctggc attccaagcc tcctcggtcc cgaattaagt gaattcttct
    62761 ccctaagatt tgctccgcac gtgattcacg caaaatctga gcctgcagat caacacataa
    62821 atgaacccac ataggaaggt attttgatgt tgtctgagtg acgtcaattg aatcgcgatt
    62881 gaaaggtaag ttcgacggct ccacctgaat ctcatcagat acacgtgaat cgaattagat
    62941 acagcgtggc tcacaccttg agtgttaacc agatgacacc tgaggatgtg gcctccgcgt
    63001 gatcagttga agccgccgat acggctatac gcgccaagcg tcatggtaac tttacgtact
    63061 tgattgagca aaccgctcgt cagccgtttc cttaatgacc attgagctac ttttcgggtc
    63121 ttaggtccgt ctctcatctt cattcgacaa aaatggcggc ccctccgttc aagcacgggg
    63181 cgccaaaaaa gacttcgcaa gaatcatccg gcaaggcgcc atctgttctg gtcttctttc
    63241 gttcaatgcg tatataaact ttgtgggaaa gctgcaagga cgctagtcct atgtgggctt
    63301 caaggtgtgg catttaaatg cccgccacat tttgtttacg taccattatt tatttagcgt
    63361 ttggggaact cgagaagcgc gcgttgaatg gcagcactgt atcggatatg cgagaatcat
    63421 tttcgtcggt cagtagcaag taatcgcata ccccgcgcct tcctccagcc aatcgatcgc
    63481 gcaaaaatat tccggagtgg tgcggagatc attgtggtac agcctgcctt gaactttgaa
    63541 aggcgattcc gtccgagccg cctccgttgc ggccgtatgc cggatagtat gcggcgtcac
    63601 tcctcgctgc agccggcgac ttttcgacta tgacgctcca ccaggtcgcg cggcatcccg
    63661 cagtctgcaa cggatcatgg cttccgtagt tcaaagctgg atggcctggg tcgtaagtcg
    63721 caaccatcat tcggcgccaa cgttgaactc tacaccttta gcaatcggct aagcatgctc
    63781 acagagagct tgcgctgcgg acacagcatt tcacctctaa ccgagcattt ttttgtggag
    63841 ccattaccat gacatcaatg atctaaggca agcagaagtt aatcatggca atggccatga
    63901 gagcggcctc tctgcaggta ttgcaaattg ggttgtgtcc gcatcggtca cagtaactcc
    63961 acaccagtga cttcacggct caccggagtc ggtcaagagt catcccccac caattcggta
    64021 gtgacgaaat cagaggatac aaagaaggat caagcttgcc taacgcccgc gattctcggc
    64081 taaactattg agtcgcgccg agctacccaa cgatgttcga attttgtatc tagctcgact
    64141 cgttcccatc gcactcgcaa atgccacgac actcgacctt gtcctcgcac gtcttactct
    64201 tgcgatgacg ccggcaggtc ccctgatgct cggcgagttg cttgtcatgg ccccagtggc
    64261 ggaaggtgac gcgagagtga tggcgatgtg cgaaacgtcc ctgggcctgg accctagaat
    64321 gccaacgtaa gttgaatgca tcggaaagac tttatctgga gcatcggtgc agtcggtgag
    64381 aaacccaaca gtcaaccgcg atcgaaatca aagcgaagga aaaatattgg tccgttgaag
    64441 cattgctctc gcccctatct tcatagatgg ggaacttcca atgggtaaga ccgcaacttc
    64501 acgaatgtgt caccagcaat cgcttctaat agggcacctc ctcctactgc acgctcccgc
    64561 caccctgtgc gtatctataa cgatacgact gacgtacctt agtatccaaa tagataccga
    64621 tatgcaattt agcgtagatg accgacagtc agtagtacgc tccaatattt cggtggatac
    64681 gtatgccctc caattacagt gcgcataatt ttcataatgg tgcgaaaatt gcttcttttg
    64741 cttctaagcc gaactgcaga actgtgcgtc tccccaaagg agcctgtctt ccgtgccgtt
    64801 tgaactctcc ttggtagatc ttgtttctcc ctacgttctc caaggtgaca ccttcggaaa
    64861 gtggcatgcc gtactctcag tctttcgggt ttcccaacac gagatcgccc acgatgagaa
    64921 cggcatcacc atccgaggta cggtcgcttt cgaaggtaac ctgtcgatcg acccaagcag
    64981 catggcgttg acctgggcca acgcggagaa tcaccctgag aaagacgcct ctcgccgcga
    65041 tccttggatt gacgtgcgcg atgccaaact cgactttcag ctggtcgtcc cacgcgtcgc
    65101 ctcacagaag gtgtcgactg cggttgctgc catcggcggc aatggggctt tcacaaattc
    65161 ggcacaggtg ctcacggcct atgacaccaa ccccaacgac gcgccgccgt ctgactatcc
    65221 caccaccgga ttcattctcg acctgcttct caccagcgta gtgctacgcc cgccgttttt
    65281 gcgcggtgcc agacgcgaag caaacgggca actggtactt gatccccaga atgaacaggt
    65341 caaattcacg cttccgcgcc tgaaattcca actggcgcag ggccaggcga acacagatcc
    65401 gcttaccgcc accctgctct ccgctggtgc gagcggcctc gacgatcctg gcgacatcgc
    65461 cgttgccgag ttgatcaaga tggacccgcc ctacgccttc atcggcccat cacaggtggt
    65521 gggcttcgga ttccgctccg gcacgctcga tctgtccgat caatccacgc cgcctgacgt
    65581 gctcgcgcag ttcgggtttg acgaatcctg gacggggctc tacctgcctg aaattcgcct
    65641 gttcgttgca ccgaatggcg cgcgcgattt tgccgtggag gcgggtgtcg agaacctgct
    65701 gattggcatc ggcacttcct ccggcgtcac tggcgacttt tcgttgcaag tgctcgacca
    65761 gggagcaggc ccgctcaaac tcagcgcgcg attctatgac ggcgaccagc gcggttacgg
    65821 cctcgtcagg acttccaaca cctcggggac cgtacagcta ccgacgcagt cgcgcatagt
    65881 ggtggacgtg gagggtggac gtgcgccggt caccgtcacc atcgcggtgg atggaggttc
    65941 cgcgcaaaac gtccgcgaaa tcgatatcga cctttccacc acccccaccc gaacgattgt
    66001 aatcaacgcc agcgacacaa gttcgccggt acgatcggcc actttcacca tccaagccgg
    66061 cctcaagcgt ccacagggtg aggtgcccgg aacctcatcg ctcggaccga cgccgacggc
    66121 gcggatcata acgcagtcca cagtccaggg tacggccacc gtgcagcaac cacgcctcca
    66181 gatcatatcg gagaccgctt ccacggcaac ggtgggattg gacgtttcct ccgtgcgcgc
    66241 cccgctgaca caatggactg tggatggatc ggcgcgtggt atgagtgcaa ccctggtcat
    66301 tgacttgtcg gccggcggac cagatgtgaa cgtggttgcg accctgccgg gggaatcagg
    66361 tgtcacggat ttcacgggct atttccgatt cgatcacccg ccctatagcg agaacaatcg
    66421 cgtgcagacc gacgaggata cgcgcgcctt cgcgctcgat ccgaacaaca gccgtaccac
    66481 agaggccaac gacgaaggga ctacgtccaa ctggctcggc ggcagcgaca tccggacggc
    66541 actactgccg gtgctccaga gcctcggccc caacacagcc gtctcgatcg agggattcgc
    66601 cagctacgaa ggccccccac ctccaaccgt ggacgaaacc aagcgagcct ataacgcgga
    66661 tctggcaaga cgccgcgcac tcggatttcg tgcagtcatc gaagacctca tcggcaaccc
    66721 agcgaacaat ctgcagggca aacaccttac tgtcacccat gcggccaaca tgacgaactg
    66781 gactaaccag ggcttccccg cagttgagac caggcgcagc tggtggaagg cagtggctag
    66841 gtggtcggcc gcgaccatcc gtgggaccac aacctcggga accctgcacc gcgatccgcg
    66901 taccagttcg aacaaaccac caaaggtcca ggacccaccg aatcaacagt ctgcaccgcc
    66961 gccgcgctgg ttcaagcaga tgggcgcgaa ggtgcgcatt gtgcgcaatc agttcgtcgc
    67021 ctgtgagatc acagcaaaga tcgatattca gaccgcagca gaggatcgcc tccaggcggg
    67081 gatgccgtca ggacggtccg gaaccctggg ttcgggacag gcattgggag caaatcccgc
    67141 cgacggaatc atcgatgtga ggcttgtgat ccagatcgat gatgccaccg atacggtcac
    67201 ggtgatcggc tactacggcg cagatccagc cgatgtcgac ggactctatc tgtggggcac
    67261 caggccagga caggctgcca caacgaaccc cggattcggc ctaaatttct tcggcactac
    67321 tatcgtgttc atgccactca tctcggcggg ggtgggcgct gtgtccaacg atggagcgct
    67381 cgccgaatta ggaatgacgg cggcgatgct tgccatcccc gcgacgatcg caggcctagc
    67441 cgaagctggt ggcagcccag tgaaggtgct ctgcgaacgc gtcatctggt acggcggcga
    67501 agtgcagttc cgcaaacgcc catccggcgc cgaagcggtg atcttgttcg acatggaggc
    67561 tgcgctcagc atggatgtct ccatcggcgg caaaacgttg ctgaaaatcg atcgcaacgc
    67621 gccgctggca gtccgctata aggctgtcgg catccgaatc ggcgacgacc ctgaccagcc
    67681 gaagttccag ttccgtccgg tctttgacgc ctcgaggggc tacacgatcg atgtgtcgcg
    67741 cccaggcgcc atcaccgtcg cctctccgtt ggatcagatc ctcaccatcc tcggcgcgcg
    67801 cattgcgcgc aacaatccgt tgattttcga gatcgacctt gggttcgccg tcgacctcgg
    67861 tgtggtgact atcgaacgag cacgcgtgcg cctccggttc gacccgctcg gacctccgga
    67921 actgaccgcg ttcgctgcag ggatcgatat tcccggcgca ctccgcggcc gtggctatct
    67981 ggagatgaac gagagcgaga tcaagggcca gatcgacctt accatcgttc ccgtgcaggt
    68041 ccgcattgcg gccggcgtgg gtgtggcgaa catcaacgag aacggccgca aggccacagg
    68101 tgtgatcgtt tctctggaag ttgagttccc tgtggcaatt ccgctgggaa actcgggctt
    68161 gggcatctat ggcttccttg gcctgttcgc gatgcactac tcgcgcaagg agccaccacc
    68221 ctccgcaatg gcgcccgccc ttgcttggct caaggacatc gctcaaggaa acccaaccga
    68281 catcgcggct tggagtccaa agatcgacac ctgggcattc ggcgtcggcg ccgtgcttgg
    68341 caccatgggg tcgagtgtca tcttcaatct caaaggcgtc gtcctgctgg aactgcctgg
    68401 gccgcggttg ctcttgatga tgcgcgcaaa cctgcttgcg gtcctgccga aactcaaagg
    68461 cacggcagaa ggcacgttcc tcgcggtgat cgatctcgac atggggcggg ggacgctgac
    68521 catcggcctc tcggttgaat tcggcatcca accgctgctt gagattcaca tcccagttga
    68581 agcgttcttc aatttccacg attcgaaaga ctggcacctc tatctcggcc gcttcatcga
    68641 acagattcat gccaagatcc tcgaagtgtt cgacggctca ggctacctga tgctttcggg
    68701 aaagggtttc acggccggag acctggcgga cggactgccg gtgccagtca atggattcgc
    68761 gatctccacc ggcctgcatg tgtcgtttgt ctggggcagt aagagcgtcg ggctctatgc
    68821 ggaagtggcc gccggcgtcg atgccgtgct cgggttcgat ccgttccgac tcaccggcgt
    68881 cctgtacctc cgcggtacct tacacgtctt catcattgat ctcagcgcct gggctaagct
    68941 cacagtcgat atcggcgaaa agccggatgg gtccaaggta gcaagcattt ctggacaaat
    69001 ctgcggcaga gtcaaattcc tctttttctc catcgagggg tgcgttgatt tcgcacttgg
    69061 ccaggcttcg ataccgccgg tggcgccacc cgagttgttt cagtccttgc gcctggtaag
    69121 tcgttcccct gccctcgcgg taggtaccgg tgtcgataag gccatcgacg gcggcatcgc
    69181 cgagggcatc aagggcgacg tcgctccggc acctcctccc aatccgccgc cagccgcgcc
    69241 gctcgggcag ccacagcccg atgtcgcgat gaatcagcgg cgcgttccga ttgatgccat
    69301 cccgctggcc atgctagcaa tgccgccggt aaccggaagc acccagtacc tcttccgcgg
    69361 tacagccgtg aatcaggcac ctggaccagg cacacccggc gctccgccag acggttggat
    69421 tcagaaaggc gacgacgtgt ttcaatacac gttgaccagc gtggaattga tcggcgattt
    69481 gctggacggt tcgacgccgg cggtctggtg gccgcagaaa gcaggcgaca caccgacgca
    69541 agctcagctc ggcctgctga gctgggtacc ggatccgact ccgaaagcaa tcgagcgcag
    69601 tcatttcctg gaggaaactg tcaaggagac ttggggcacg gtgtgcttcc ccgccgcagc
    69661 gcccacttca gtactgttca cctttctgca tgagccgttc ggtccatcaa cctacggatg
    69721 ggtactcgac ggcaaaccgt ggccagatcc tccggatacg attcgttctg ttccaccgac
    69781 actcacactc aaggtctccg agcgttggcg aacggcaagt gatgaagtgg atgaactagc
    69841 cggtgtgatt cccgccgtgg tagaaggggc accggtggtg tgtcagcaca aaccgaacca
    69901 acccgacacc ccgattcacg ccatccctgg taccgtcccc atcccggtgc ccacccgggc
    69961 gctccgtttt gatccgatcg cggcagcccg tggagtcaaa actcccgacg ccttcacccc
    70021 ccaagaaatg acgcttgtgg atctggcgcg ccgagccaat gcgggcctcg aagtctcgcg
    70081 ttcgatgatg accacgttgg tggtcacacc gaccacggca gctgccactg cagaccgccc
    70141 ttgcaccagc cgcgtactag ccgcgccagt gctcgactcg aagaatgcac agccgttcgg
    70201 accaccctcg cggctcatcc agctccagaa ggagcggagc agacgcgggt tcaagcccgg
    70261 ccccttagat gatgcagtgg tgttctacac cggacccgtc gagaccgcga cgttctatct
    70321 cttcgtgccg cgcgacctcc tcgcgcagaa gatcgtggtg gtggcggcca cggacgggca
    70381 agacacggtc ttccagcagc tggtacttga cccgtccatg atgagtccgc ccaatacgct
    70441 gcctccccgg tggatcgatg cgtccgggcc gtggttcgag gaagcggctc acctagcgca
    70501 acatcagagc gtgctgcggc cacgcggctt cgtcggcgtg atcgtcacca tcaaggggca
    70561 ccagagggcc gaccgcatac aaatcgggct gacccagcac gatgcagact ggcaccgcaa
    70621 aacagttgaa cggccattct acgtagcggg catcgaggta ttgacggtgg ccgagtttgt
    70681 ccggttcgac tacgacacca aggaacagaa gaagaaacga ggcgtgcttg aagcggcact
    70741 gagcgacagc agttccggcc aggcgctgct caaacccaat acggcctatg gggtcaaggt
    70801 cacctacgac gccaaacgag gcaaacgtcc acccgggcaa gccgtgagtg acgaagtgtc
    70861 ggctctcaac cagcatcaaa cgttctggtt ctatacggac gaccagccgc caaggcggct
    70921 cgacccctgg ctgatgtgca gtacgcccga agacggggag caccactact tcggcaggct
    70981 gcctgtgcac atcgtgttca acagccccga tatggggcgc atctacgacg catacggaag
    71041 gcgactgcag atccggctgc gcgctgcttc tttccggccg ttgccgagta gcccgacagc
    71101 gccgcacccg ctcccgttga caccggattt cctaaaaccg gtgaaggcgg cactactctc
    71161 gccatgggaa ggagtggtac aggaccagct catcgcctcc tgcgttccaa tcagcgtcga
    71221 acgcgctcgg cactccatgg tgacgatgcc gattcctctc gagcccttca ccgattacgt
    71281 gctcgatatc gagatggcag acaagaacgc acctaacgac gcaccaggtc ttgtggtgtg
    71341 gcgaaccgga ttctctaccg gacgcttcgc gacggcgccc gcctttgccg aatcattcca
    71401 gctcaaccgc ctgctgcacc gcttttccaa accgggtcaa ctgcaggcga ttggcacgca
    71461 gccctgggcc gccaatccgc agggtaatca gcttgatcag gccatgatgg cagctggtct
    71521 ggaacccatg ggtgtacccg aagcacctcg gatcatggtc ttctgggaaa cggctgcgtc
    71581 tgacccgcag cctgccgccg tcctcgtcga ttcatcggaa ccgatgtggc gcagccgcag
    71641 gatccccaaa gaggcgatgg atcctaatcc gtctaacgcg aaacgttacg agatgacgac
    71701 cacggaatgg ttacggctgg aggaagaagc aggcggcgat gcgatcgtgg ataaggtgat
    71761 ccaagcgcct ggtggacaac gtgcgctcgt caccttaaag ccgaactcgc gcggcaagac
    71821 cttgcggctt gccctgcggc tggtcgcaca gaaggagccc tatctcgacg gcccaaccgc
    71881 tgccgatcaa ttgttcaccg tgatcaacgc acaactcaat cgcgcgccat gggaggaaga
    71941 ggactgatgg cgttcattgt cgacataacc aaattccagg ccgaaggagc gttgttgaat
    72001 tggccggtgg tcgcgcggcg gctcggaatg ttggacgcca cgacgggggg gattcagctg
    72061 ccgtcggtgc tgcagctgcg ctggaccttg aataacgaat tgggactccc cacagaaccg
    72121 ttcatcgtct ggcggcggaa caagaaactg cgcgggccga aaaccatcgc cgcggaggtg
    72181 accacgatcg ggctgctcgg caattcgcaa ctactggatt tcaaggcctc ctacagcaca
    72241 gtggaattga acgtctccgg aagtggagga gtcgtctacg cgtttgtggg cagtccgtgg
    72301 ctcaacagca tcgtagcaat cgcctcaatc tctgtgggaa acaacatcgt agtgacatta
    72361 gcggcacctt ctatggaagg tcttatcgtc tccagcgggg tggtggtcaa caacatcgcg
    72421 ggcgtccgtt ccgacgatct tacagtggcc gacggctggc agaaggtaga gtttgtcggc
    72481 ttgccggtca aggaagacga ttgggcgggg cagggaatag gacggcacgg cgtggaccaa
    72541 ggcatattcg gagcgttcac ggatgcgatc tctgcggcaa aacaacgact agagcgaggc
    72601 gcccctccca tcggctgggc tccagccatc gaatccggag tcgcggctcc gccatggagc
    72661 gcgcccgttc cgaaccagtt gctcaacgat ctgaatgcga tgctcttgga cgatctgcgc
    72721 acagtcgcac ccctgccgcc agcgactcag gccgccaaga aaatcagtaa gccgatgccg
    72781 ccgccggaaa actcttccgg cgaccagatg tccgggcccg gcggcacatc gcaggtgtcc
    72841 ccgctcagtt cgacctatat ggcggcggcg atcgattgtt tcaattgcct tgcccttggt
    72901 ttcggaacgg catatcccct gcgcttatcg acgacgggac aagcggagaa cccactcgat
    72961 ttcgactata tgatcacggc acagtacgaa aagggactcc ggggcaacgg tcctgcagtg
    73021 gcatatgcgg cgctcgtgcc ctcgccggga caggcgattg cgccgccgat tcccgccaac
    73081 atggcacagc aaatgatggg caaccttcga ccgctaacgc gcgatggaag ttggctggca
    73141 tcggtacgga tctcctggga taggccagtt ccgatttccc tgttccgccc gcgatcgttc
    73201 gccttcgtcc gctccgaggt gacgccgcca tctcccgccg tcctcctcat gaacaaacgc
    73261 tccgacgggt cacctctgcc ggtcgccgta aattacctag tgactcccca ggatccggaa
    73321 cccaaccggc tgagtgcggt ggagcgcgag atcccgattc ccaataaccc cggtacccgc
    73381 acgataaaat acgccgccgc ccatcaagac atctacggtc agtggagtag ctgggtggca
    73441 atcaacgcgt cggtcaatca acccgacgtt gatgagcctc ggatcgtctc cgcagaattc
    73501 aaatatacgt ccgttccctc gcctcccgcc ggtatctgcc atgcgaacct cgtgctggag
    73561 ttcctctggg attggaggat ccgcactccc ctcaccatca acttccgggg acgcttacac
    73621 gccgccgcct atcacggtgc gccgcccccc aacatcacat taccgtcggg actgcaggtg
    73681 aaactcgaag tcgcgccaat caacaccttt acgttgaagt tcgacctgct cgccgccaac
    73741 ggcgcgccga cctcgagttg gcccggctac gatccgttga cccactgcgt tgcgctgagc
    73801 caggcggggg accagcaggt gccctttggt catgcccagg gaggcgaaac acgccgctat
    73861 cgtgtaacga ttcccgggct cgaactgagc ttcgggccaa ccggccatat cgggctcgcg
    73921 ctttgggccc aagggcagga atccatcgca ccgcaacgca acggcaattg gagcaaagaa
    73981 ccgagtgtga tctccacctc cgatccgcga ccgccactga ttgcccccga catcgtgaca
    74041 ctctccagtc tcccggatgc agccggcgaa tcccacgccg ttttgaaatg gtccggctct
    74101 cccggtgcag acgggtactt catttacgag accacggaga gcaagttatt gaaagccgtg
    74161 ggcgaatcgg aaccagaccc atcgaaaacc ctcagccagc gcctgaccaa actgcgtgcc
    74221 atcttcgatg cccaccccgc agagcgccgg accgagttca cgcgccgcaa cagtcgcctg
    74281 atcaaaacca cctcgtccga catcactctg ccgcgcggct ctaccgcgat tcacctcttc
    74341 gccgtcctgg gtgtgagcgc gggccaggtc gaagcatcat ggcctatcgc ttcgaccgca
    74401 ctctacgcgt tcgccgtacc gcgtgttccg aagcccggcg cgccgacaat cgaagtatcg
    74461 agtttctcgg acatgaacgt cacaccgaac gtttatcgct ccaagatccg gatcgaaacc
    74521 cgcaaggggc cgcgcgtgcg tcgcattgat ctccatcgcg tccgcgtgga tgatgcggca
    74581 agggacctcg ataccatggg accgcccgta ctcaccttgg atgccatgac acctggctgg
    74641 caggttgatc ggatcaacga tgctctcggc gagcatatta ccgtggccaa gggaaaagaa
    74701 acgcctccgg ggtcctggaa gcgcgtttgg tacagagcgg tggcttggtc ggccgacgat
    74761 cccctgcggg gcacacttgc cgcgcgatcg ccggccagta ccgtagcctg ggtggtgatc
    74821 cctccttcaa ctccacccga tctttccggc atcaccatgg aatggcctgg taccgcgccg
    74881 caggacgtga tgttgaagtg gaccagcatg acaccgacca agaaaacccc ccttggcccc
    74941 cataccctcg cggttcggat caagcgcatc ggtgcaccgc tcgaagaagc gtcgctcctt
    75001 accttcgacg gccccttatc tgacctggcc accatccagg cacccggcgg gtctgcggta
    75061 tggcggatcg aggccaccaa accgacgcaa taccgagcca tcatacgtcg cgccgacatc
    75121 aatgacgcga ttcagatcag cctgcgcctg accgatccgg tgggccgctc cgcggaatca
    75181 ctcgccacca ttaatccagg ccacatcctg cccgatcctg tgctcgccga tgtcctactg
    75241 aaaaagagcc tgaaccctcc aggggtgatg ctcagctggt ccagcgacac tcccctcgaa
    75301 ccaccggttt actcggtgcg cgtcaccgtg aaccaaccgc cgaggctgat cggcagcttc
    75361 aggattccgc agcctccgat cattcagcag atgtcgttgg ccaccgtgcc gatggacgag
    75421 ccgggaccgg tcccgcttgg cgccgatcca ctgcgcgtcc gccgtatgcc tggcgccgga
    75481 cccaagtata catactatgc cttcgttcga gtctcattca cccaaatcat cctccggctg
    75541 agttcaccgg atgggcgttt cgcggaacac atccaattac caagctgatc accatgccac
    75601 cagtcaccat caccaccctg actccgactg ccatacagca gagcctacag agcaacggtc
    75661 tcgcggcact cggcctcacc acgttctcgc tgagcccacg atggggggat accacgccca
    75721 tcgcgggcga ctacgacaac aacaccctca ccctcacaat cacgggcctc atgcgcgcgc
    75781 cattcctcag catccgcgag aatgccttca ccgatgcaac atctaccttg gccgccgacc
    75841 ttcctggccg cgacaccgaa acgactgtga cactggcccc aggtacgggc aacgcctttc
    75901 ctgccccgac cggaggtgga cgggtcttac tcaccctctc gaacagttcc gggaacaccc
    75961 gagaggtcgt ggaatgtacg gcccgaacgg gcgacgtgct aactgtcgtg cgcggagccc
    76021 atggcacggc aaagcaacac ttcagtagcg gcgatcgcgt gacattgcgg ctcactccgg
    76081 cccagaggtc gagtgagttc tccggagtcg atggagcgcc tctgaatgtg gcctgcacgg
    76141 ttttccgcct ccacccacag gcctaccttc gtctgcaaac cctctgtacg acgcgctacg
    76201 ccgctccagg ccaacccttg accctgccgc tgcctgccac catggtcgta cggggcgccg
    76261 ccggcttccg tgcttcccgg tggtaccgcc ccgacgaacc actcgacgac gtcaccgggg
    76321 aaatatcctt ccacgacaac cgtggattca tcatcgaccc gatctacgtc gccggcctct
    76381 tcgccgatct gctcacgacg ttgcctggcc tggtgcctcc cgcagtcacg gcctctgcga
    76441 acgggccggg aggggtgcaa ggtatatcga tcctcaccac aggcatcctg gtgcatttta
    76501 tcgatccaca cggcaactcg ccacgcatcg caacgccggg tgtaaacttg atcaccacca
    76561 atgccggcac caccgtctca ggcaacctca tcccgctctc tgaaggcgac accatcgcgg
    76621 ccaatagcgc cacgacgcca ctgcgctttg gcttcgccac gaacggcacc atgagtacgg
    76681 cgcctctttc tatcccagcg ctcaccaatg gaaccattgc acggcgcttc tatcgcgtca
    76741 tggtggtaga tacctcctgg tacctgttgg gcaaccgaac ggcgggcacc gtcctcggag
    76801 tggggcctga tgatcaacgg attcccacgg acctccttcc cgtcgtgcgc gaccaagtga
    76861 ccatcgacta cctcgtcgat ggaccggata cgctcgccga agccactcgc attctgacga
    76921 ggccatccca gagcattgtc ctggcggttt ccccagtcat cgaccaaacc cttattacag
    76981 ctacgcagcg aggcacaacc gcacactggc caggcttccc gcctccaaac gtcaacgctg
    77041 gcttcccgaa tccgccggta cgattgtcct cgaccaatgt cactgcggct ttcacggctg
    77101 gcaatgatgt ggtggtgacg gtcgcaggcg gtgccgtgcc agacggcgcc accatacgca
    77161 tctatccaca agtcttcatc gaaattgcgt caatcaacgg atccgaacct tcgttcgtgc
    77221 gcggagatgg cggcgcgaac atcgttaccg gcgccacccc cacaaacatc ttgctacgca
    77281 accctttcaa tctcggggcc gtccagccac atccaagtcc cgcgattctc accatggaca
    77341 tcgtagtcgt gccgcgcaat ggcagaagac ggctcaccgc tgcagtcgca gtgcctgtgg
    77401 cagcaggtcc ggctaccgtc ccgactgatc ctttcttcgg accggtcggt gccaacatca
    77461 tgggcatcat gctgccggtc acccaaggcg tcgcccagtc gccactgttc ggcattccca
    77521 gcgccatcac gccaccagcg gttcctccca cgaacttact ggaattggca ctgagcctcg
    77581 cagcggaacc agccccgagg caggcgcccc ggctacccac gatgggacgg ttggagacgg
    77641 tcgccgtgac aggcacgacc gacgcaggcg cccaaccatc agggtccctg ctttggcagg
    77701 ccgtcctcac tggtgcccgc tgggcccccg aatcacgctc cgcccaacat gccgaaggta
    77761 accccggcaa tccggctggc cctgatgtgc acgcgcccgg tgtctcggtg accggagccc
    77821 tagcctacga cctcgcattg cacgcgctga agcgcgcgca gtcgctgatt ccgcgcccac
    77881 tccccggcgg tggcatgagc cctggttggg tcttctcttc cgctggggac aatttcgatg
    77941 agccatccga tatagccaat accgcaaata ctggtatggg cgtcctcctg gagacggtcg
    78001 ctgtgggttg cgagacctcc tggctggcaa gcctctcgcc gccacaacct ggattcaccc
    78061 tgaatcagtt ggtgcagaat gtggcctcgg ccatgtcgct accgaccccg cctacgaatg
    78121 cgatcacggt cgaaaacgaa ccgcgcattc agcggcagat ccgccgcgag ttcttcgcgg
    78181 cgaagcacgg tttccgcgac gctcaatggt ctctgcggcg agccttcgct gaggcacgtg
    78241 aactggtcta catcgagtca ccacaatttg cgcggacagc ccgtccgtcc ggctctccgc
    78301 aaaacttcga actggacctg gtagccgcgc tcgcggcccg gctcgcggca caccctaccc
    78361 tccgcgtcat catctgtacg ccgcgatttt ccgacttcga cggaaccttc cgcagctttc
    78421 accgccagca ttaccaggca cgcatggaag cgctggggaa tttgaaggcc gcggccgggg
    78481 accgggtgct actgtttcat cctgtcggat ttcccggacg agtggcctac atacgaacca
    78541 cttcgatcat cgttgatgac gtgtggtgtc tggtcggcgc cacccatttt agacgccgcg
    78601 gcatgacctt cgatggcagt gccaccattg catcattcga tcggcagatc atagacggat
    78661 actcaaccaa ggtacgcaac tatcgccgca cattgatggc ggagaaattg aacgtaccgg
    78721 ccccatcccc aggccaaccg ctgagcggcg aatgggttcg actcggccag cccgtctcga
    78781 cctttgagct agtcagggac ctgttagagc agggtggtct cggccggatt cagggcattt
    78841 ggcccggtcc atccgacaac agtgttttgc cggcacagcc ggaggtggcg gatccggatg
    78901 ggtcgaacgg tgcgacgatg ttgaccacac tcgccggtat gttgaatgag ctaggtacct
    78961 aactttggag gaagcggcta ttggcaattg actgccctgt caggggaagg acgaagccgg
    79021 cctctacttt taaattcaca atggtcaacg gacccacaag cctccaaatg aaggcactga
    79081 catacagtgg cactataatt tctaggggcc atcgccccgg gagaaagtca tgcgaaccac
    79141 aattgtacga cgcaacgatt tctggattgt taccctagcg ttttatactc tcctcagtgg
    79201 ctgcggccca tcgcccctgc cgactgcggc ctgcccccaa tcgtacatca aacctatatc
    79261 actccaagtc gccgcacaaa gtgcgacaag cactgtggac cttgctctca gccaatctta
    79321 tatgcggaaa cgcattaaag acacaattca gactccagac acggattcac aaggacatgc
    79381 gacctcacat ggtatcgatg tcaatcagat tacactgctc gaaaagactg tcaatggtca
    79441 ggatctgcac ttgatcgcca tcaacatgac gccatggctc agagggcaaa atggggccca
    79501 cgtttcatca aataatacct atgagctctt tctgaaattg aatccatata tcgtagatca
    79561 gaacactgtt cctgatgcgg ctcaacgtaa gaaagtctta tgtggaacaa ataacacatg
    79621 tactgagaat gaaggggtgc ttttgggatt tgagcttcat gagctcaaaa acaaaagtca
    79681 gacgactgtg gtggcaactg gcaacgggat ttgcggtaca gtgaatctca tcgatgagaa
    79741 ggtactcgac ggaatatggg gcatcgatgg tggtctgacg aatgcgacac cagtagtatt
    79801 gccaactgca tcaattgctc aaatgattac aggattcgcg ggagttcccg ttcgtgtgac
    79861 aggattggcg ttgggaagta acacggatct gaaaattgga atgttgatga acgcaggcgc
    79921 tggtcatgca tttgactcaa gcaccggatt tcgaccagcg gtagattgga ctttagacct
    79981 agatagctca gtgattaaag acttcatatc gatgacggca aatgcagtcg ccgcaagtaa
    80041 caaccctccg gcgaccgtaa gtgacatcgc agtgaaattc atcgaccaac cagaaaacgc
    80101 cgttgacgtc gtcgtcaatc ttacgcttcc catctgtagt accgccaaag ttcgcgtatt
    80161 gtctcacgtg cgattcgatg tatgcaccga tatgaactct caaccaatcc ttcagcaatg
    80221 taaagctgac gaccaaagac tgcctaataa ttggccaacc aacgcctgtg tgattgccga
    80281 tcacctattc aggtggccat acatcggcaa cgccattgat cctccgtgtg gattggcgaa
    80341 ttcgtcaacc gtgctcaagt ttcaggttgg acctgccgag gcattctatg cgacctccat
    80401 ggatgtctcg gacattaact ttcacatcga cggccgtagc actgtaatgg ataccgttgc
    80461 agcagcccag ggaatttcta gagttgccaa gccgcacgct tgtgattcaa gctttagttt
    80521 tcattttcct taactgatag gcatcgagaa ccaaacagcg ggcgaatttc ccacccgcat
    80581 aaccaaattt tatgtcggtg atttcaagtt ccacttgcac cttgcgaatg atggcgcagg
    80641 tggaagggaa ctccggaaat ttgggcagtg tggtaagtgg tagcctggct tcatcaaagc
    80701 caccaggcgg tggcgaacca gaggagcgag gcgatgaaga ggacttcgca cgaagcatcc
    80761 ggcaacgtac cacccgtctg gccttcctca gttttatgcg cagatagagt ttctggaaaa
    80821 tccgtaagaa tcgcggatca tagggacggc ttcattgacc tacgaccttg aatgcacggc
    80881 tctgccgtaa tttacatacg tact
//
LOCUS       DAORRQ010000011        79685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_11_length_79685_cov_30.367198, whole genome shotgun sequence.
ACCESSION   DAORRQ010000011 DAORRQ010000000
VERSION     DAORRQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 79685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 79685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79685
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11_length_79685_cov_30.367198"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PK224_05255"
     CDS             complement(<1..984)
                     /locus_tag="PK224_05255"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HRB15173.1"
                     /translation="MVQVDNMTLSVPLEQLQDNDALLSREWLVTNGLGGYASGTLLGA
                     PTRRYHGMFVPDLPSPWGRTVMIPRLDEEVVVEGESVLLSGIEFEDGRLESDLTKVIS
                     AFTREWQTPVWRCSFKGRRLEKRVIMPYGHNSVYVEYRLLEGDPVRLHLRPFVTFRML
                     DAPLHDARKPPFPLTVLDGRYEMSLCENAPSLKMCLRPHAGVFVADPLLSPGVSYRVD
                     RDRGAEHVESLASPGYFSADLMPGLPIAFVSSTEPWEHLEFTPEAIFDAEAQRLSKLV
                     AQLPDASQHDIERWLTLAADQFIVLPGSRMEEQALARASGDEARTVIAGYHW"
     gene            complement(1036..2943)
                     /gene="treZ"
                     /locus_tag="PK224_05260"
     CDS             complement(1036..2943)
                     /gene="treZ"
                     /locus_tag="PK224_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HRB15174.1"
                     /translation="MSGSATVVPEQPHGNQAPSAGRKRLGAQVIQDGVQFRCWAPKPA
                     QLDVVLEPHGTVHPLTRGADGYWTGVIPEARAGMSYRYRLDGNSLYPDPCSRYQPDGP
                     HRPSLIVDPDTFAWRDRGWPGLTMHGQVIYELHIGAFTPEGTFDAAVTRLDTLKDLGI
                     TVIEIMPVAEFPGRWNWGYDGVGLYAPAHVYGDPDALKRFVDEAHLRGLGVILDVVYN
                     HLGPDGNYLPAFSDEYLTDRYPNEWGQAINFDGPGSKEVREFFIQNACYWIEEFHLDG
                     LRLDAVHAFHDEGPLHVIAELSQAARKAAGARSIILVAECEAQWVCTIRPTVERGWGL
                     DAVWSEDFHHSARVAATGRSEGYYTDYRGTPQELISCVKRAFLYQGQRYQWQDGPRGT
                     AVRAEPAEQFVFFLQNHDQVANQLRGDRLHQKTGPGLHRALTALLLLAPETPMLFMGQ
                     EFGASAPFLFFADFPAGELARQIHQGRKQFLAQFPSYASAGAQAAVADPRDPAVFQRS
                     KIDWSERERHAEHVRLHRDLLRLRREDPVIAQQARDRLDGAVIGPAAFVLRYAGKQGD
                     DRLLLINLGADCDYRPAPEPLLAPPDGRTWHLLWSSDAPEYGGPGVIEPLTETGWVIP
                     GESAALYSVQG"
     gene            complement(2940..4082)
                     /locus_tag="PK224_05265"
     CDS             complement(2940..4082)
                     /locus_tag="PK224_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HRB15175.1"
                     /translation="MNRPMKSACGLTAIKAYSHPSDENSARSAKVAGLRYVLDRSPGI
                     ARKGRGRNARYVRPDGTVIRDQTCLKRIRSLVIPPAWTDVWICMDPQGHLQATGRDAR
                     GRKQYRYHPRWNQIRNESKYDRMLTFALALPRIRRRVAADLRRSGLSRDKILATVVRL
                     LEATLIRIGNEEYARTNESFGLTTLRDRHVKVKGSTLRFEFRGKSGIRHSVDITDGRV
                     ARIVKQSQDLPGYELFQYVDEEGRRRCITSGDVNEYVRRIAGEAFTAKDFRTWAGTLL
                     AARALRKYEDQCDSLAKAKRNVAAAIDAVARQLGNTKAVCRKCYIHPEIVHAYLDGTL
                     LKSLGPNIHNGVPGSSQQLPREEAAVLRFLRNRILLSVGAAKGIAS"
     gene            complement(4079..4939)
                     /locus_tag="PK224_05270"
     CDS             complement(4079..4939)
                     /locus_tag="PK224_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRB15176.1"
                     /translation="MPRSLWKGSISFGLVNIPVVLYSAENRNSFDLTLLDRRDMKPVG
                     FKRYNKETGKEVAWDHIVKGYEYENSRYVVLTDEDFRRANVEATQTIDILSFVRAEDI
                     SPTSFETPYYLAPDKRGEKSYALLRETLTKTGKVAIATVVIRTRQYIAALIPWSEMMV
                     LNTLRYANELRPIKDLEIPAKSLKATGVTPREIEMATKLVEEMSDSWKPVQYKDTYHD
                     DLMRLINKRIKAGKTEVVTAPDKGEAGEKPGRAKVVDLMALLKRSVQQSGQPKRHLPK
                     RERQAGRKTA"
     gene            5616..7070
                     /locus_tag="PK224_05275"
     CDS             5616..7070
                     /locus_tag="PK224_05275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB15177.1"
                     /translation="MCIVGAGIAGLTTAYCLMKEGKSIILLDAGQPGGGMTQRTTAHL
                     SNALDDRYVEIERLHGETGAQLAAHSHTAAIDWIDRVRFEERIECDFTRLDGYLFVPS
                     DNARHLIEEEWQAARRAGVDGVERLDRLPGNMFGTGPCLRFPRQAQFHPLKYVSGLVK
                     AILAGGGRVFSNAHVTSVASDTQVKLETSQGSVVTADAVVIATNTPINNLVTIHTKQA
                     AYISYVIGARIPAGSIEPALFWDTLDPYHYVRIHRQVSAREGEDSVLIVGGEDHKAGQ
                     ADDGEARYARLEAWARERFPMMNAVVFRWSGQVMESVDGLAFIGRNPGDTDNIYIATG
                     DSGMGMTHGTIAGLLITDLIMKRESPWSSLYDPSRRPVRAAGEFIRESLNVAAQYTDW
                     VTGGDVETEAQIPHAQGAVLRDGLTKIAIYRDAQGELHKCSAVCPHLGCIVAWNHAEN
                     TWDCPCHGSRFDKFGKVLNGPATSDLSPLKQEDT"
     gene            7271..9004
                     /locus_tag="PK224_05280"
     CDS             7271..9004
                     /locus_tag="PK224_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRB15178.1"
                     /translation="MRYLALAADYDGTLANAGTVGPDTIQAIERLVASGRKLILVTGR
                     VLPEILEIFPQISLCERVVAENGAVLYRPATKEITLLAPPPPSAFLEEVQRRKVEHLT
                     LGNSIVATRVPYETVILDIIRDLGLELRIIFNKGAVMVLSHGINKATGLTAALKQLEL
                     SPHNIAAVGDGENDHAMLTYSEYAVAVENAVPMLKETADRTTVGDHGHGVIELINELV
                     ENDLAVADRSVARHRIALGTQENGGDITFHPARQNLLLAGTSGSGKSTLATGLLERLG
                     ERGYQLCVIDPEGDYENFPQAIVLGTAQDGPSHAEILTALANPNNHVVVNLVGLPLQD
                     RPSFFLTLLPKLQELRSKSGRPHWMLVDETHHLLPVDGNPTTPGLMKDLAGMIYVTVH
                     PDHIEHSILKTVDIVFALGKSPDETLKQYCAAIQQPAPAATAARLQPGRAIMWNRASG
                     ETPFVLEIAPSTIERRRHRRKYAEGELPPEQSFYFRGPAGQLNLRAHNLLLFMQLGEG
                     VDQATWIHHLRSQDYSTWIKQVIKDEALAQRVHDVEQQAHLPAEESRQLIRSAIEERY
                     TVPAGGDEHTS"
     gene            complement(9274..10140)
                     /locus_tag="PK224_05285"
     CDS             complement(9274..10140)
                     /locus_tag="PK224_05285"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HRB15179.1"
                     /translation="MKRTHMWGTVRTAALCLLSMGVLLSGCALTFGYRHADWMISWQL
                     DHYLDLTAGQRRDVTARLKPLLARHRTEAIPQYEQFLKELQQRVSRGLTREDLEWMYA
                     SYDRFREDLFERAVPDGSALLMTVSERQVRNLEDVFRKEEHKFVRTLQGSTSKRLEER
                     TRKMLATAEDWLGPLSVEQVARISPFTRALPDTQPVIWQHRRQRQQELLTLLRRPASF
                     EDMSRELRMIFVQPDQTAPAGYRELVGELRASLTVLLLEADRLLTVQQRRKAVTSIQR
                     LIDDLHGLSPGS"
     gene            10354..10791
                     /locus_tag="PK224_05290"
     CDS             10354..10791
                     /locus_tag="PK224_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15180.1"
                     /translation="MKTALHSLLALALVSLAACSHSPTASKTRSEMEAGTKTGMVKNI
                     VIKETIVPDALTVRAGDEVRWINQRQDSVTVTIDEPLEHNVSCRNGFSKAMGMGVDNS
                     TTLSSNETAGLCFSRIGTVKYSVRPADETKTSMANTHGSITVQ"
     gene            complement(10884..13079)
                     /locus_tag="PK224_05295"
     CDS             complement(10884..13079)
                     /locus_tag="PK224_05295"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRB15181.1"
                     /translation="MPKDRSKRENEKTAALDSAREKSGDHFLTTDQGLRINDDQNSLK
                     AGARGPTLLEDFILREKITHFDHERIPERVVHARGAAAHGYFQVYKSLASLTRAKFLQ
                     DPQVKTPVFVRFSTVAGSRGSSDLTRDARGFAVKFYTEEGNYDLVGNNIPVFFIQDAV
                     KFPDLVHAVKPEPHHEMPQAASAHDTFWDFISLMPESMHMIMWVMSDRAIPRSFRMME
                     GFGVHTFRFVNAKGVGRFVKFHWKPLLGVHSLLWDEAQKISGLDPDFHRRDLWEAIEA
                     GQYPEWELGVQIVEEKDEQKFEFDLLDPTKLIPEELVPVVRIGKMTLDRNPDNFFAET
                     EQVAFHPGHLVPGIDFTNDPLLQGRLFSYTDTQLIRLGGPNFHEIPINRPLAPLHNNQ
                     RDGHMRQTINSGPVSYQPNTLGGGCPMQAKADMGGFLSYAERLPGSKVRERSKSFFDH
                     FSQAALFWRSQSEPEQEHLVQALQFELGKVENLPIRERMVGMLSQIDKGLAGRVAEGL
                     GMPVKKIEGLLNLSIPADGDPKDFQPKRAMTSLDKSAALSMVLAPKEGIQTRKVAILV
                     ADGVDGEAVKGMQMALVAAGGQGKIVATRSGSLKAANGSELKIDFSLLTTGSVLFDAV
                     YIPGGEQSVSALLRTPKARTFVQDAYLHCKAIAATGEGVALFEAAVGAAYPEAEHGSR
                     TRQTPKDWSQDSGIVLGPDSSPKQIAARFMKGIEMHRHWAREIRLASPS"
     gene            complement(13115..13615)
                     /locus_tag="PK224_05300"
     CDS             complement(13115..13615)
                     /locus_tag="PK224_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB15182.1"
                     /translation="MARAAGFVSTVFEMLKEDHDKVKKLFEDFESAEGRERGEIAKTA
                     IRELEIHADLEERLIYPAIRAEVDEADMMNEAIEEHHLVHVLIAELKKLKPGDEKFQA
                     KFSVLSELVKHHIEEEEGEMFPKAEECDIDWESLEASVMKRKETLLAKASGRKTGARP
                     SRKTIR"
     gene            13824..14063
                     /locus_tag="PK224_05305"
     CDS             13824..14063
                     /locus_tag="PK224_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15183.1"
                     /translation="MTTPRFIPICCGCNNVRDDSRRDEREPGWGTLDAYLHTHHLTSG
                     DYRLTHTYCPSCSVRFSKLSGGHAVTPEVAASRSL"
     gene            complement(14203..14892)
                     /locus_tag="PK224_05310"
     CDS             complement(14203..14892)
                     /locus_tag="PK224_05310"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRB15184.1"
                     /translation="MRLRVASYNIHRAIGRDGRYEPGRILRVAQELNADIIALQEVDF
                     PRQDHAPIAPWLAQKTGMIAIGGPVWSREGVGYGNALLSRYPVDRVRRWDLSVGRHEP
                     RGALDVELTICGRTVHVLNTHLGLWPRERPQQADRLLELLSLSRRDLTILMGDLNEWH
                     GWGKSLRGLRRVFGSPAAPVTFPARWPVLALDRIWIFPAQALLTVEAHDTPRSRLASD
                     HLPVKADIYLP"
     gene            complement(14914..17121)
                     /locus_tag="PK224_05315"
     CDS             complement(14914..17121)
                     /locus_tag="PK224_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRB15185.1"
                     /translation="MERAEVLKACQAQEQTPPSFLVRGRTCWRVERSDRCGFLIDGEA
                     YFKTFRDVALHAKHSIMIVGWDLDTQIELVRGPAEPSEFPSKLGEFVSALLRRKRKLR
                     VHVLNWDFAMIYALEREWMPTAQTGWSGHRRLSYQVDGQHPIGASHHQKLVVIDDTIA
                     FVGGLDLTKSRWDTPAHACQDPLRVDADGQPYAPFHDVQMMVSGEAAAALGELARDRW
                     FNATGRRLPLAIQRPVEELWPVALVPDVEPCGVGIMRTQPAYAGQPEVREIELAYLEG
                     IKRARRSIYIESQYFTSNAISRALAARLMEQDGPEVVLVLRHSCDGWLERQTMDALRS
                     RILHELELADHYGRLRIYAPTVPGTQGRDMVAVHSKLLIVDEDFVCLGSANVSNRSMG
                     FDTECNLAIESGGTSRLQAVIARLRNGLIGEHLGVEPELVAEHVQRLDSLGPAIDHLR
                     GGDRSLEDGCFDKSPAEAISIPEQLLIDPERPMAADEVLGAVVQHRERAHVGRRVFLG
                     YTVLLLLGLLAAAWRWSPLSEWMNVNDLVDAVRALGQGPTGSLVMLGGFLVGGFLAVP
                     ITVLIVVTILAFGPLVGAPSALAGALLSAVTIFGLGRALGRYRVQRFAGRRVAHLSRR
                     IAQRGFWAILIVRLLPIAPFSMVNLVAGATSLSLRDFLLGTALGMSPGIVLMSAFVDR
                     LAEALRNPSPVTFGVLGLLMAAAWSVAWYVSRRLQVRDTPPDVGNVQQVAPAA"
     gene            17810..18268
                     /locus_tag="PK224_05320"
     CDS             17810..18268
                     /locus_tag="PK224_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15186.1"
                     /translation="MLRTIIGVGLVSGALLTGWSPSWNEVPAPVPHPGPGPTVPPKPS
                     DPVPLPPDPKPVLPPHPANTPHAYLMADPATDPQNTVSGVLEKVDLIAKQGLLKTDLG
                     REVAFQIPKPELFLNLSVGQRVTLKLDDRQQAVRVMDTTAPEIPPPFPAK"
     gene            18439..23193
                     /locus_tag="PK224_05325"
     CDS             18439..23193
                     /locus_tag="PK224_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15187.1"
                     /translation="MNSSDSVSWLTGSEMGGRIRTFDWRRTPLGPIEDWPAALVSILG
                     VCLTAQYPMAIYWGSEGWLLYNDAWRPILGDKHPWALGRAAHEVWPELWDTISPLLHS
                     VQTTGQAVWRGDELLPMQRFGYTEECYFDYSFNPIRGQNGAVEGILNIVQETTYRVLN
                     DRRMRLLRELASRSGFAHSQEDACNLAIEALATDQADVPFALLYHIDRDRRHAHLIAS
                     TGLPPEHPARQQTVSLTPEEPDSGWPLSAPLQEGVPVIVDDAGDRFGPLPGGSWSEPT
                     GQALLLPLSTVRWDGRAVILAAGINPRRPLDDDYRSFFTMVESHIAGALTNAEAYSSE
                     KRRAEALTELDRAKTAFFSNVSHEFRTPLTLMLGPLEEELRERPHAPRLEVAHRNSLR
                     LLKLVNTLLEFSRLEAGRVEARYEPTDLAGYSAELASQFRSAVEQAGLVFRIDCPPLP
                     ETIYVDRDMWEKIVLNLLSNAFKFTFQGQIALSLHAQTGGVELHVQDTGVGIPPEALP
                     RLFERFYRVQDMRSRTYEGSGIGLALVQELVQLHGGTVRVNSQVDQGSTFVVTLPFGN
                     AHLPPDKVVPHGRPTTPTTGVGPYLEEALHWLPEPQHATKPADGRPDPAEAPLSTTSA
                     ETARPRVLVVDDNADMRQYITRLLADRYHVIAMPEGMSALIEARRQPPDLILSDIMMP
                     QLDGFGLLQALRDDPALKTTPVILLSARAGEESRIEGLGRGADDYLVKPFGARELLAR
                     VATHLDMARVRREAQREIAHSEARFEAIVSQATAAVAQTDLTGRFLFVNQRFCELLGY
                     SASELLGMNMQQVTDPDDLPANLVLFHRLTAGGPDFVIEKRYRRKDGSAVWTRVSVGG
                     VREDGELRHILAVGLDITDRKRQEAELLALTHRQSLLYDLADVVNRAEALTGLYEKAI
                     DTIIEALHADRASILLFDDEGVMRFEAWRGLSQAYREAVEGHSPWIPDQQDPPAIFIP
                     DVAASDLHTSLRSVIQSEGVSALGFIPLTYGGRLLGKFMMYFDRPHVMSEQEIGLAQT
                     IARTLALGIERARAGELLRSNEERLRLAMAAGSMGAWDADLSTGATDWDAKQYELFDR
                     PKDKPLTHAEQFYESIHPDDVPRVREAAAQAELTGSFSQEFRVIAQDGSIRWLSGHGT
                     AVKNDQGQAVRMVGINYDITRRKHVEEALQRSASDLEQRVVERTLELSRSREQLRALA
                     TELNLAGQRERQHLATELHDYLAQLLALSRIRLAQAKQQPMTTALGKILSELQDVMDQ
                     ALTYTRTLVAQLSPPLLKEFGLPMALKWLAEQMIQRDLKVSLELGTEHLALPEDQAML
                     LFQSVRELLMNIVKHASAKQARITVREEEGWLHITVADQGRGFSPPDSIPETGSTGIP
                     GFGLFSIRERMLALGGRFELQSHPGQGTEATLTAPLTPGKARAKQPAEVDGTVPSIAP
                     LNTKHQAAEREGPVGKRETVERPIRVLIADDHAMVLQGLCGLLAAYGNIQVVGEAADG
                     EEAVTLTIQLQPDVVLMDVNMPQMDGIEATRRIKQVLPATIVIGLSVQNAAHVGRVMR
                     EAGAAAFLNKEAAVEDLYQTIQVARKELLQVP"
     gene            23190..26309
                     /locus_tag="PK224_05330"
     CDS             23190..26309
                     /locus_tag="PK224_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15188.1"
                     /translation="MIRFQQAPNPLLSGAIVFSLATVFVLDFYTPLGITVWVLYLIPV
                     VLAYLFWFPVTPVAVAGAVTILTIAGLFLSAPGIAPSVSRINRLVGIITSWALAAFGA
                     HFIRNRLVMRREEWIRAGQTLLSERMMGELALNSLGENLLHCLAKYLGAEAGAIFIDD
                     GRGFHRTATYGVPEGTAMPERFAAGDGLLGQALQHKHTVVVHDLPEGYFTIGSALGRG
                     APRHLMIAPLTVDGRVNSVLELGFFTRVTESDREFVDRVAESIAVAVRSARYRTRLQD
                     LLEETQRQAEELQVQSEELRVANEELEEQSRALKESHSRLEQQQAELEQTNSQLEEQA
                     QILEIQKEDLNQSTAILESQTRQLERASRYKSEVLANMSHEMRTPLNSTLILTKLLAD
                     NPQGNLTADQIKSLTTIESSGHDLLALIDDVLDLAKVEAGRIDLTPQPVPIPRLLDSL
                     RSIFKPLAEQRGLRLTMQQRPGTPDSFETDSQRLEQVLKNLLSNAIKFTEVGEVSLEV
                     SRLSDGRLAFTVQDSGIGIPQDQHELIFEPFCQADGTTSRKYGGTGLGLSISREFVRL
                     LGGEIHLTSTPGQGSSFTVLLPQRLSSAPFPAPARSLHPQSPPKPMRSGDIPPIPAIP
                     SESLVATTHIEDDREQLAANRRVILIVEDDEPFSKILADLAHELNFQVLVATTAADAL
                     ALASRYLPSAIILDIGLPDHSGLSVIDRLKADPRTRHIPIHVVSGHDYERTALSLGAV
                     GYMLKPVKREQLVEAFRQFETRLTDKPRRVLIVEDDPAQRESLRLLLGSDEVETIGAD
                     SAAECLAHLAERTFDCMVLDLTLPDASGFSLLETLSREEHLAFPPVIVYTGRELSPDE
                     EQRLRKYSSSIIIKGAKSPERLLDEVTLFLHQVVTALPPEQQRMLEKARSRNAVLESR
                     RILIVEDDVRNIFALSAVLEPHGAKIEIARNGRESLRILEESLQHDDRLIDLVLMDIM
                     MPEMDGLTAMRAIRERPEWDTLPIIALTAKAMKNDQEQALAAGANDYMAKPLDVDQLL
                     SLVRVWMPR"
     gene            26306..27145
                     /locus_tag="PK224_05335"
     CDS             26306..27145
                     /locus_tag="PK224_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HRB15189.1"
                     /translation="MTSDTAMKTEDIELQLFLEAVYQQYHYDFRGYSKASIKRRLLLA
                     RERIGCTTFSGLQDRMLHDHAVLPQLLDYLTVQVSDMFRDPGYFRALREQILPHLKTY
                     PSLKVWVAGCSSGEELYSLAILFREEGLESKTMFYATDINAGALAKAEAGVYDLQRIA
                     LFTRNHRLAGGKGSLSDYYRAAYGAARFDKTLRRRTVFSDHSLVSDSVFAEVHLVSCR
                     NVLIYFDRPMQDRAIGLFKEALVRKGFLGIGARESLRFNAHVKTFTELLPGERIYQKR
                     GDE"
     gene            27142..27747
                     /locus_tag="PK224_05340"
     CDS             27142..27747
                     /locus_tag="PK224_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HRB15190.1"
                     /translation="MTALPLHECLREPRAIVIGASAGAVAALSRLLPPLAADFPLAIL
                     VTVHVPADKPNSIPALLQTKCRIAIKEAEDKEPIVPGTVYFAPPDYHLLVEQDRRLSL
                     SNEEPVNFSRPSVDVLFESAADAYGKDLLAIILSGANEDGARGARAVREAGGIVVVQS
                     PDSAEARMMPESALRACPKARAMNLHTLAELLCACGGTVQC"
     gene            27741..29822
                     /locus_tag="PK224_05345"
     CDS             27741..29822
                     /locus_tag="PK224_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15191.1"
                     /translation="MLTDSPAKFLLVDDHEPNLVALSAVLSRDDVEMLRARSGREALE
                     LLLQHDIALAVIDVQMPIMDGFELAELMRGSRRTQHVPIIFLTAGPQDNLRRFRGYQA
                     GAVDFLYKPIEPDVLRSKTGIFLDLYRQREELARQRDTFITLAEEKARLLRERDEANR
                     RLRESEMRFRTLADSAPVIIWMNGPSGCEFVNQTCLDFLGIDHLGQIDIFDWARHVHP
                     DDRDQAAAVYRQAVEGRTRFEASLRCRRRDGTYRWIRSVGMPNLSADGGVLGYIGASF
                     DVTESKEAEERLQRWSVDLERAVSQKTSELLQSQEQLRALATELNLTEQRERKRLATE
                     LHDYLAQLLVVVRMKLRQTIHLVTGERIPELLKDADHALTQSLDYTRSLVAELTPPTL
                     KEFGLLQALTWLATQMHRHGLTVTVRQDAAPLPLPEDQAVLLFQSVRELLFNVLKHAK
                     AAEATVVLGLREDGQLEVVVADDGCGFVTAPKNRPGTTANRFGLFRIQERMAAMGGQL
                     VIESVLGGGTRATLTTPYWNSREKPEDDDTLTPAESRAPSLRTRSTDVEHLSHSHHPT
                     SKIGILLVDDHAMVRQGLRSVLEHYDDVAVVGEASTGREAIEFVERLRPAVIVMDINM
                     PETNGIDATVEIKARHPDVAVIGLSVHNTAEARKAMLQAGATTLLSKESAVDELYAAI
                     RQALADEGVPR"
     gene            complement(29831..30214)
                     /locus_tag="PK224_05350"
     CDS             complement(29831..30214)
                     /locus_tag="PK224_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15192.1"
                     /translation="MKQTLGGEIDGMRLNDSRQASAQTTAGSLGSSRAVQQGPLIEVS
                     ESDGTFNVYADFRAMPEMASAEEIAVEFARQGIILSSGSHQRYVPIPTDAQIEHARVT
                     IDAGIVRMSVPTADLGHRWRSIVMW"
     gene            30429..32408
                     /locus_tag="PK224_05355"
     CDS             30429..32408
                     /locus_tag="PK224_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15193.1"
                     /translation="MHSTDGDNPTASEIPLQIDLLLSRLAAIVESSDDAIISKDLNGC
                     IMSWNQGAQRLFGYTAQEMIGQPVHRLIPQDRLDEEPRILERLRLGQRIDHYETVRQR
                     KDGTMIDVSLSVSPIKDRGGRIVGASKVAREISERKQGERDLANLVEALPVAVYTTDA
                     EGRITRYNQAAVDLWGSRPVLGTTRWRGSWRVYWPDGRPMPLAACPLAQCLRLNRPIR
                     GLETIIERPDGTRVPVAPHPTPLRNAAGELVGGVDVLMDLTERKRAERQLQESAAELE
                     RRIKERTHELVLSQERLRALASELTLTEQRERRRLAVELHDYLAQLVVASRLRLSQAI
                     PKVQDETASAALTNVDGMLDQALTYTRSLVAELSPHILYQFGLSKSLVWLGERMKQHA
                     LEVTVKTGPTSFTLPDDQAVLLFQSVRELLFNIVKHAKTDRAVITINVDEQRELWICV
                     EDEGVGFDVTELTDGEDSRGKFGLLSIRERMETLGGECELSAAPGAGTVAILRLPLSG
                     SATASPSTPPTTVPSHTPAAASKDPARRIRIVLVDDHAMVRQGLRSILDGYANLIVVG
                     EGADGQDAVVMVRSLRPDVVIMDVNLPLIDGIEATRIVSREYPSIAVIGISVRNDPQV
                     HVAMTEAGAVAFLPKESAASQLYEIILRHCPAIAI"
     gene            32627..36172
                     /locus_tag="PK224_05360"
     CDS             32627..36172
                     /locus_tag="PK224_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15194.1"
                     /translation="MTSMLLTLQRVVLSAAGLAIAFPALTLLCWAYDHSILYHLHTSF
                     RTLKPTTMLAVLFCGLSLWLQAEEGAVTTARRYVAQGAAIVACLLASLILSEHLLALD
                     LGIDLWRIALPEISSDAHPGRTAPATAVAILMLGLTLLWLDTKPFATYFFDQSAALIG
                     GTIGCIGLVGYLYGVQSLYKVGTFSSTSVYSALLLVTLGLGILCARPARGLMATLTSE
                     KLGGVMLRRLLPFAVMLPILAGWIRIAAQRSGKYDFNFGVALAVAAVMLIMLAFLWLL
                     AGSLNHTDGQKKHIMQILQQREARHRLALRAGRMGTFHQDLDDESLVFSPELEAIFGL
                     PPMSFASTFAAFQQLIHPDDRQRVQQTVAEAVENRTAYDLECRILRTSPPGESWIAVT
                     GQVLPDSTGHPRQITGVMFDITERKRSETALRQSEEQLRVVTDALPGLIAYVDRDERY
                     RFVNAGYERHFGRSREQIVGSSVKELLQQDYPQVQRFIERVLEGEQISFETTSLGETD
                     EQTLLATYVPERTAGGTVEGFYVLITNITDRKRTEQALRESEERFRHLFEQASDGIVM
                     ADMSGHYLDVNSRACHMLGYSREELLGMGVTDLLDPHDYKRLREIKAELLEGHRHSGE
                     WKLRRRDGATLSVEISAKLQPDRRWHAIVRDITERKRAEAGLRESEAHFRMLADATPV
                     LVWMAGTDTRCTWLNRSWLEYTGRPLEDELGSGWTSGIHPDDQAEYLRVYKAHFSRHE
                     PFEMEYRLRRQDGTYGWILDRGVPLLTQNGEFSGYLGSAIDITDRKHAEEQLQQWTGE
                     LEKRVDERTQALVRSQERLRALASDLSATEQQERRRLATELHDYLAQLLVVGRMKLGQ
                     AKPHVQDSKTLQLLSEADDVLTQSLNYTRFLVAELSPQVLYQFGLPAALKWLGGQMKT
                     HGLTVIVHCDRDHLPMDEESAVILYQSVRELLFNVVKHARTTEAAISLSCRTDDIVTI
                     TVSDQGCGFNLSTMTETDRALPGRFGLFNVQERVEAIGGRLNLDSTEGKGTTVTLTAP
                     IDGNSQSEPGAVSSRAKPLSASPVPACERLRILLVDDHEMVRQGLRSVLDNYEDLEVI
                     GEAGDGESAISIAAALKPDVVVMDVNMPRIDGIEATRRIVAAQPDIVVIGLSVQNERH
                     VEEAMTRAGAAVFVTKERAAGQLYEAIVRTVRTRK"
     gene            complement(36182..36349)
                     /locus_tag="PK224_05365"
     CDS             complement(36182..36349)
                     /locus_tag="PK224_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HRB15195.1"
                     /translation="MLSWAVTFLVIGLIAGVLGVSGVAGTATHIAYVLFVVFLILAVI
                     GMVMGKRPPAV"
     gene            36424..36642
                     /locus_tag="PK224_05370"
     CDS             36424..36642
                     /locus_tag="PK224_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15196.1"
                     /translation="MTQPLCPQAQAPLGADELRQASEQNSRPFSAGQEHPGCAQDFCP
                     LAGSGIMTSFQTTEAVFHDGVAEPNEHP"
     gene            complement(36742..37824)
                     /locus_tag="PK224_05375"
     CDS             complement(36742..37824)
                     /locus_tag="PK224_05375"
                     /inference="COORDINATES: protein motif:HMM:NF018439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HRB15197.1"
                     /translation="MIPPWKVVLLACVTCAFVAAGAAAAGSDEYIAGYAAATLEHEFH
                     LSGAVIEVDRGAVTVQVKRLGGEDPAKIVRALEQIPGVVRADVQAGKEPVPSSGGTAA
                     RAVTPEAESKFLPRGLLVDPLHADLRWPHFGAAYHNLSSGKEFASAFGESFAFYRNAA
                     PFDGQWELGMQAGVFGVFDTEKRSIDLINADYMVGLLASYRADKLSGFFRVRHQSSHL
                     GDEFLLNNAQVARINLSYEEIDLKVSYDLTTWLRVYGGVGTIVRKDPSTLGRETSQGG
                     VEFKSPWLFFGGKVRPVVYADFQANARTNWNVAQSIMGGLQFENARIGDRKLQVLAEY
                     FSGPSPDGQLFTQKVEWIGMGVHLWF"
     gene            complement(37911..38510)
                     /locus_tag="PK224_05380"
     CDS             complement(37911..38510)
                     /locus_tag="PK224_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRB15198.1"
                     /translation="MKTVSPSILMLVLGLTLAVGSMAAPTGAQAADDKGLFEQIDIGL
                     LSIGGRATYVDPKDGSSRWFGGGQVRLHPSRYFAFEGSVDYRRNDIGDTRVHSYPVMV
                     SALIYPLGTTRLAPFLLGGGGWHYTTVKGPGGFDDTQNRFGAHAGGGLQFFFNKHVSI
                     DSTYRYIWLEKIESRDQNIVDKKFQDNGHMVTVGLNFHF"
     gene            complement(38545..38904)
                     /locus_tag="PK224_05385"
     CDS             complement(38545..38904)
                     /locus_tag="PK224_05385"
                     /inference="COORDINATES: protein motif:HMM:NF017353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRB15199.1"
                     /translation="MKLRMRLLSMTLTGMVLTSGLSQAGDRSFGSTHGADEHPGTVTV
                     AVNQDQLQGKWKQFKGELKQKWGDFTDDDLLAIEGSVDKLEGKIQERYGDRREDVKRW
                     VDEWFDAHGTQREQTSS"
     gene            complement(38971..39249)
                     /locus_tag="PK224_05390"
     CDS             complement(38971..39249)
                     /locus_tag="PK224_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRB15200.1"
                     /translation="MKKIIAMALMSGVLASPAWALFETNKQLASTATVTLEDAVRHAL
                     KAVPGKAVEAEIGKEDGRTVYEVEIVDANNKTQKVYVDAQSGQTKIDH"
     gene            complement(39317..39556)
                     /locus_tag="PK224_05395"
     CDS             complement(39317..39556)
                     /locus_tag="PK224_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRB15201.1"
                     /translation="MNADQFKGKWVQFKGEVKKQWGKLTDDDLMQVEGDYDKFVGRVQ
                     ERYGDKKEEVVKWADDWYAQQGKPRTEGRQAQPQR"
     gene            complement(39553..39786)
                     /locus_tag="PK224_05400"
     CDS             complement(39553..39786)
                     /locus_tag="PK224_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15202.1"
                     /translation="MKHLKFKGVKQTVVVRTGEDDGRNTVVLYRGGKTGLAFRTPDHR
                     VFREAAQQSRGGDAAISASPASAAMTSQQGGSR"
     gene            complement(40026..42167)
                     /gene="glgX"
                     /locus_tag="PK224_05405"
     CDS             complement(40026..42167)
                     /gene="glgX"
                     /locus_tag="PK224_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRB15203.1"
                     /translation="MMKVWPGRPYPLGATWDGEGVNFALFSENATSVELCLFDGPDAS
                     KESHRIRLEERTNQTWHAYLPEGRPGLHYGYRVDGPYEPGAGHRFNPSKLLIDPYAKS
                     IADTIRLSDRMFGYRIGDPEGDLARDDRDNADESPKCVVIDQAFSWGGDHLLRTPWSK
                     TVIYEVHVKGFTARHPDVPEHLRGTYAGLSTPPVIEYLQGLGVTAVELLPVHHAVQDK
                     HLTDQGLTNYWGYNTIGFFAPDMRYALSPVRGRHVREFKTMVKTLHSAGIEVILDVVY
                     NHTAEGNHLGPTLSFRGIDNAAYYRLMGDDPRYYMDYTGCGNSLNVRHPRTLQLIMDS
                     LRYWVLDMHVDGFRFDLASTLARELHEVDRLSAFFDIIHQDPVLSQVKLIAEPWDLGA
                     GGYQVGNFPVGWAEWNGKYRDAIRRYWKGDGGQVAELAYRLSGSSDLYEMSGRRPFAS
                     INFITAHDGFSLQDLVSYNEKHNEANGENNQDGHNDNLSWNCGAEGPTEDRDVLELRR
                     RQKRNMLATLLLSQGVPMLCGGDEIGRTQLGNNNAYCQDNEISWHNWDLSRENRTLLT
                     FVRRLIALRQDHPVFRRRRFFQGRRIHGSDITDIVWLHSDGKEMDEADWNQGHLRAIG
                     LVLAGDAIEEKDARGNAIVDDTVVLLFNAHHESIPFVLPACDERTSWVLMLDTCDPTP
                     RRSSAVFKGGEPYTIEARSMAILCRESVHGA"
     gene            complement(42220..42759)
                     /locus_tag="PK224_05410"
     CDS             complement(42220..42759)
                     /locus_tag="PK224_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRB15204.1"
                     /translation="MKPAKPQVSIIKDVQTIRERARQHIEEGAMTFDYKADRETVIKL
                     LNETLATEIVCVLRYRRHYFMAEGMNAESVKSEFLAHAGEEQAHADQLAERIVQLGGE
                     PNLSPEGLADRSHSEYIEGENLEDMIKEDLVAERIAIESYRDMIAFVGSDDPTTRRLL
                     EEILMKEEEHAEDLVSLLK"
     gene            complement(42809..43000)
                     /locus_tag="PK224_05415"
     CDS             complement(42809..43000)
                     /locus_tag="PK224_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15205.1"
                     /translation="MPFDTTGKQVGLFLSLAACALVVGTQSKGLLQPATDRGPGVEPA
                     AELGLESALPHLRVDLAAP"
     gene            complement(43137..43604)
                     /locus_tag="PK224_05420"
     CDS             complement(43137..43604)
                     /locus_tag="PK224_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB15206.1"
                     /translation="MKHEGPRTMEEARARRDRSSLYLESTLDEQLGLLPGEARKVKSA
                     MSSAVTVASPKTSLKEAMSMMTSLNVPVVVVYDGRRLVGMLTDRDIGLNRRVQEASQD
                     APISGFMRTSVPCCLEDDLLADAISVMHGSQSDWLPVMNRHEQLVGVLSVHAS"
     gene            complement(43634..43930)
                     /locus_tag="PK224_05425"
     CDS             complement(43634..43930)
                     /locus_tag="PK224_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15207.1"
                     /translation="MDSHEKVMTIRRWIHPEERITVDFEDERDLNAEVIECESQTVTL
                     LLETAFPHYKQHLTLPLSIVSIGEDKTKYTRNPDKPVEYGRLRLTVHEVRPQAV"
     gene            complement(44035..44238)
                     /locus_tag="PK224_05430"
     CDS             complement(44035..44238)
                     /locus_tag="PK224_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15208.1"
                     /translation="MKLLVIRPDPDPQPPDPAPFPGPDPGPFPPQPFPPPIPPLPTRP
                     PIPQLLEQEFMCRDPVPVVSEMA"
     gene            complement(44270..44884)
                     /locus_tag="PK224_05435"
     CDS             complement(44270..44884)
                     /locus_tag="PK224_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HRB15209.1"
                     /translation="MRKIDPYTAKSYDLFGLSGISDHSLAVHFGLYEGYVKAANSLRA
                     RLHEILSDGTVDQEEMPAYSELTRRLGFEYNGMILHEYYFGNLRRGGMDRPADGSPFG
                     QAVERTFGSYDRWKTDFCSVGMLRGVGWAICNIDPSTGLVSNHWVSLHEHGNVAGFLP
                     ILVMDVWEHAYLLDYQPQERKQYIEAFFSNISWSAVEERLQAAC"
     gene            45195..45851
                     /locus_tag="PK224_05440"
     CDS             45195..45851
                     /locus_tag="PK224_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB15210.1"
                     /translation="MSKARLVIADDHSIVLEAYRQLLEPEFEVVGTAENGQELLNIAP
                     GLAPDIIMLDISMPTMNGLDVTKQLRAAVPRAKLIFVTMMSEPFYISQAFELGASGYV
                     LKQSASTELLSALNAALKNQRYISPQLSLEVQDAIETPWVKPEGFSSKLTPRQQEVLQ
                     LLTKGFSTKEIAATMQVSTKAVEFHKGNITRRLGIRTTAELTRFALSQGLTKLDDQHP
                     "
     gene            complement(45856..46539)
                     /locus_tag="PK224_05445"
     CDS             complement(45856..46539)
                     /locus_tag="PK224_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB15211.1"
                     /translation="MKPRVLIADDHTLVAEGVEKLLEHDFQLCGRVADGRALVRAVEK
                     EKPDIALVDIALPLLNGLDACRQIKKSTPEVKLLVLTMHGEQYFVTEAFRAGVSGYVL
                     KQSVAEELVFAIKEVLKGRLYVSPSVAENLVEQALNPTEPGPARATATNDQLSARQRE
                     VLQLIAEGQSTKEIASTLNVSIKTVEFHKTRIMKQLGVHSTAELTKHAITIGLIAMPQ
                     QPTVPGPHV"
     gene            complement(46630..47907)
                     /locus_tag="PK224_05450"
     CDS             complement(46630..47907)
                     /locus_tag="PK224_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15212.1"
                     /translation="MARVLACGLGLVLCGLGGNVAQGEDPTPIAGEEFRTTLFGEEIY
                     VPPRDRRSVTAASFGIQAIPNGPSQMEMLPFGALYVWRNWDDDNRRLRGTFSGVVNDV
                     DYTIGLREYPNWSLIFTWDNFILPMGRSEYVQGQRQRGTELEWSYAFAGAGLGYRLPV
                     HPFRQDSAVNMSITYEAGYRWFKGTGHTSAQYGVPKDTYEGRIHFRIRADALKRNLME
                     LPHEGFTMGGDLIHGHRAKWDRWGGAPFDTPDFRKEQTYLQASAYALAATGLPFIESE
                     KHRLLTSVHGGIGKDLDRFSAFRLPGRPTGYEWEAISLPMIHGVAFNELFPTRYAVAN
                     LQYRYEALFFLYPYLEATWGLVERPVFTSNGAVKNITDSMPALGGGLVTGAPWKSQVE
                     LNYTYNFGIYRDPGGAPPTKGGHGIIFFWSKEL"
     gene            48313..49065
                     /locus_tag="PK224_05455"
     CDS             48313..49065
                     /locus_tag="PK224_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15213.1"
                     /translation="MCLIATNELLAAPIEVIYPEGVSEGFVTLKSMDGKKLADGELSQ
                     LTTGADRLASRLTFRFTDGSLYDETVTFSQKKHLAMLSYQLNQRGPAFPEPLTISLNG
                     ETGQYQVRRHEQAKTEQTISGRIDLPADIYNGMTITALKNLRGRSGASIHMVVFNPEP
                     KIYELDLKLVEHEETRNTNRKEVKATAAHYLLTPKLGWFMSALASLVRRTLPEYHFWL
                     IKKDAPAFVRFEGSLYPDGPIWRIEQVSPRVK"
     gene            complement(49080..49775)
                     /locus_tag="PK224_05460"
     CDS             complement(49080..49775)
                     /locus_tag="PK224_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15214.1"
                     /translation="MHRKSLLAFTAVCWFSMTSPIEAFPGVASGKVSDSEQAADDEAA
                     SSFAADGVVERYLIDPRGEVEGLLLVDGTYMYVTSRAADHLTRALKPDDHVQVFGRRQ
                     SNERLVQPDVIENLTRGTTFIVPLRVDLPMQEQARRLSVTEMNATGTIRLLLYHPIKS
                     TVQGMILSDNTQIRLPLDASEALRRSFQIGDAVVIKGNGTTNQFGRAIEVVAIGRTPA
                     SLVPVDAALLSLP"
     gene            complement(49817..51607)
                     /locus_tag="PK224_05465"
     CDS             complement(49817..51607)
                     /locus_tag="PK224_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRB15215.1"
                     /translation="MPSKIEDYALIGDTHTAALVSRTGSLDWLCLPRFDSPACFAALL
                     GTPEHGRWSIAPNQDITTIRRRYRGDTLVLETHYVTGTGTVRVVDFMPPRDQDINVVR
                     IVIGVEGTVRMKMEMTLRFDYGSIVPWVSTDGRLFKAVAGPDAVYLETEVELRGVGFS
                     TVADFTVSAGEEIPFTLTWRPSHDVPPKPLSPKRACSETESWWGKWSAVCTYQGPWRD
                     AVVRSLITLKALTYAPTGAIVAAPTTSLPELLGGARNWDYRYCWIRDATFTLYALLMA
                     GYRGEAKAWREWLLRAVAGKPSDLQIMYGISGERRLPELELNWFPGYERSAPVRIGNA
                     ASRQFQLDVYGEIMDTLHQARNLGLAPDEDAWRVQHALVEFLESAWDRPDEGIWEVRG
                     PRRQFTHSKVMAWVAVDRAVRAIERCRVEGPLERWRALRATIHDQVCREGYDSGRGSF
                     VQYYGSTALDGSLLMIPLVGFLPFSDPRVQGTLDAIERELTVDGLVRRYHPSGEVEGL
                     LGEEGLFLPCSFWLVDNLILAGRRRDARNLYDRLVGLANDVGLLSEEYDVKNKRLVGN
                     FPQAFSHVSLVDSAYGFSRAESPVEHRQRG"
     gene            51785..52132
                     /locus_tag="PK224_05470"
     CDS             51785..52132
                     /locus_tag="PK224_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15216.1"
                     /translation="MRKIVAKKTEARNEKSATPRRAKQRAIEPATHTIKRKPSTGSSA
                     ATAQKKRVKKQDGKMPNVVAKRASSSTRRAKTIGRAVGKILGRVIGTVEQTVAKVIPR
                     PKSPAPRKTGTSR"
     gene            52645..53427
                     /locus_tag="PK224_05475"
     CDS             52645..53427
                     /locus_tag="PK224_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB15217.1"
                     /translation="MSARGTFVSILAWPLKGLVRTILSPSLYQQVRVTFLGEQSGLGL
                     DWRWANSLRRPHPIRRDFGWQAGQPIDRYYTEQHFLPACRADIRGRVLEIGDDRYTRQ
                     FGAEKVTVSEVLHLHPDTPGATICADLTKADHIPSDSFDCIILTFTLQFIFDVRAAIR
                     TVARILKPGGVALLVFPGITQISRYDMDNWGEFWRFTSLSARRLFEEVFRPDDVTVTT
                     YGNVLAATASLQGLISSELRREELDHWDRDYELVIGLRAVKR"
     gene            complement(53438..54721)
                     /locus_tag="PK224_05480"
     CDS             complement(53438..54721)
                     /locus_tag="PK224_05480"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRB15218.1"
                     /translation="MQRVVIVGGGFGGLAAARALEGAAVQVVLVDRVNHHLFQPLLYQ
                     VATAALSPADIAWPLRTLFRGQQNIRITLGEVTKIDRAARCVLLSETAPIPYDRLILA
                     PGARHAYFGHEDWEMHAPGLKTIPDALELRERMLMAFEQAQQAGDAQRASDYLTFVIV
                     GGGPTGVELAGALMEIGRRAMAPDFPVLHRAEFRVILIEGGGRILEAFPPDLSGKAQT
                     ALEALGVTVMVNCRVHDVTGQGVLAGAQFIRTANVLWAAGNIASPILRSLDVPLDQTG
                     RVRVERDLSLPGDPWTFVIGDAAHCESPEGRALPGVAPVAMQEGRYVAGLIAHDIRKE
                     HRLFFRYRDRGMLATIGRAKAVARFGPVSSSGLVAWLAWSFIHIFFLIGFRNRFRVMV
                     EWIWYYITFKPGARLIYGKPRGLREEGAGEANGLP"
     gene            complement(54864..55571)
                     /locus_tag="PK224_05485"
     CDS             complement(54864..55571)
                     /locus_tag="PK224_05485"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRB15219.1"
                     /translation="MMRQIAIYAGTIALLLATSSPGFTQGSREGGTSDSTMGKGAGTS
                     TPRETINPDKKTDLTGGKQGVPEEYADTPVRQGKLEEEKGSKYANASVYNTQGAEIGK
                     IHQVLKDTKTGDIEYVVFVPNESKRPIPIRWSQFVTKGDKLQLNLKKEDIQNPVSMNS
                     SKDMSPDIKHYMDKMEQVREAPSAPGNPGIPGQKGPGATGPMGEESVGGGGPSGTSGL
                     PPGQAPGFEGGHPSSKR"
     gene            complement(55628..55987)
                     /locus_tag="PK224_05490"
     CDS             complement(55628..55987)
                     /locus_tag="PK224_05490"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRB15220.1"
                     /translation="MALHTGRRLLAASTIEGTPVQNQAGEDLGEINELMIDLEKGRIA
                     YAVLSFGGFLGLGDKLFALPWEALTMSAGGDFFILNVARERLEQAEGFDKDRWPDMAD
                     TAWGERLHTYYGYKPYW"
     gene            complement(56119..59013)
                     /gene="treY"
                     /locus_tag="PK224_05495"
     CDS             complement(56119..59013)
                     /gene="treY"
                     /locus_tag="PK224_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HRB15221.1"
                     /translation="MPRIPVSTYRLQFNHTFTFKDAAALVPYLHGLGITDCYASSLLK
                     AAPESMHGYDLVDPGVLNPELGSEEDFAHFAEALKQHDMGLLVDVVPNHMGIGTPDNR
                     WWWDVLENGPGSRYAAAFDIDWTPLKRELEDKVLLPILGEQYGTVLENQEICLQYEEG
                     GFLVSYYRQRLPLAPKSWAAILSFRITELIELLGSGHAAILELQSILTALSHLPPRRE
                     RDPEKVAERYRETGIVRRRLAALITDYREVHAHVLANVETYNGTKGLSASFDKLDALL
                     NEQSYRLASWRVASEEINYRRFFDVNELAAIRTEEESVFTESHRLIFRLLKRGIATGL
                     RIDHVDGLYDPEHYLQQLQAWAAAELPREREEDTPSLFVVVEKILGEGEQLPRSWPVA
                     GTTGYDFLNLVNGLFVRADQEQAMEALYTRFIGERRPYRDVVYQSKKLIMRASMSSEL
                     NVLGHQLNRLSERDRHYRDFTLNSLTHAVREIIACFPVYRSYLTTDREAPLDRDQAYI
                     VLAVARAKRRNPTLNGQIFDFVRDLLLGKLDPSTGLTKEDQIRFVTKFQQTTGPVMAK
                     GVEDTAFYVYNRLISLNEVGGDPAHFGSSVEAFHQAIRERRAGWPYSMSATSTHDTKR
                     GEDVRARINVLPELRERWSKAIARWARLNRRYRTEVEERPAPDRNDEYLFYQTLVGAW
                     PLMTMDEVRYEEFVTRIERYMIKAVREAKTHTSWINPHPDYEAALCRFIRAILSSREP
                     NPFLNDFLPFQAVVARYGMYNGLSQLLVKVAAPGVPDCYQGAELWELNLVDPDNRRPV
                     EYALRARMLKEFDGAATNEAGDRLEFIRCLAESWQDGRLKLFILQAALRHRRAYPDLY
                     LDGDYVPLDCEGPHQFHLCAFARLHQDQAVVAVAPRFMAGSAATRDEGRPDDTWRDTW
                     LTVPSWKAGSAYEHLFTGERFETVNRGERQVLPVGQVLKHCPVALLIRSD"
     gene            complement(59038..60297)
                     /gene="glgC"
                     /locus_tag="PK224_05500"
     CDS             complement(59038..60297)
                     /gene="glgC"
                     /locus_tag="PK224_05500"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRB15222.1"
                     /translation="MGTHARLNQPRVLGLILAGGKGERLMPLTRARSKPAVPFGGTYR
                     LIDFVLSNFYNSGITAMHVMVQYRSQSLIEHLRRAWRIGDGDRQFVTVVPPQMNLHRG
                     WYEGTADAVYQNLNLIHDFTPDIVAVFGADHIYRMDVRQMVRAHQEREADVSVATLPV
                     PMASAQGFGIVEVDRSGRIVGFEEKPSEPKGLPGQPDRALSSMGNYLFNTEVLIRALE
                     DNAEREGTHDFGKDILPRLIRQERVMAYNFQENDVPGLGYYEEPGYWRDVGTIPAYWQ
                     ANMDLLGDTPLNDLRNPEWPIRSEPSFGPPASVVNCYLDHAVIGEGSRLIEADIRRCI
                     IGRHVRIDAGAHIEDSVILDHARIGAKAHLRRVVIDRNNEIPAGVVLGHGDREVGPNA
                     SWMASDVMVFPKPDVRAELFKRRLSSH"
     gene            complement(60481..61938)
                     /locus_tag="PK224_05505"
     CDS             complement(60481..61938)
                     /locus_tag="PK224_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15223.1"
                     /translation="MRTSRRRRAATVGLLGASVFGIALVSGLVFVGAQGHGEEKRNQP
                     QSVIEKAKEKGFDGVDFSYDLFGAPSGQSTAHIAEEVMRKDMADKPKVMANHAALLQA
                     RYTLECKTQAGAVMTKGKPQPIGPTVQLKEGMTWDKLSHISAEDIRRQKVFPAGFMRL
                     PHVKHEVGGQVFPQKQIEQFPRLERFDIDFDLPDCFLPEFPPPIFLTTHPELGDVSQG
                     EVLSADNFDRLFRGLITPVQLDGLRMLVTQFPQEEFNLTPDRKSPKPSLGVACLDCHV
                     NFHTTGQFHLNPDTRPQRDRLRLDTVSLRGMYNQQVHGSKRSLRSIEDFTEFEQRTAY
                     FNGDPIRAMKKGMNVIDRLQVAHMAQIQNMIDFPPAPKLDPMTGRLDRARASQQEIAG
                     EDLFFGKARCVTCHQPPAYTDHMLHDLFLERFGAEADGPIKSFALRGIKDSPPYLHDG
                     RLLTLEDTVEFFNLVAGLQLNRDEKSALAAFMRAL"
     gene            complement(62388..62921)
                     /locus_tag="PK224_05510"
     CDS             complement(62388..62921)
                     /locus_tag="PK224_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRB15224.1"
                     /translation="MSIGMRDKAVFVVAREMAGSGGMYSRAASATAGETERRAYRLLT
                     LAGFTIVIFTSDALPVYAEGDEGNRTCDDVRHSTRPAPLSRQIMPFLDATAALHIDLA
                     CSWLVSDRLDNIEVGRLLGCNTILCLNGSETDWDMTSMRWPHLIAGDIWETACLIVNA
                     GRASVASVSDEESDQDD"
     gene            complement(63203..63403)
                     /locus_tag="PK224_05515"
     CDS             complement(63203..63403)
                     /locus_tag="PK224_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15225.1"
                     /translation="MVTLLVAIVLIGMISTALYAFGIFNTPYTDAFRIGFYLILLLGL
                     VAVVLTIGRHPIEGYDPTQATP"
     gene            complement(63588..63935)
                     /locus_tag="PK224_05520"
     CDS             complement(63588..63935)
                     /locus_tag="PK224_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15226.1"
                     /translation="MWPPAVTGMLGVWLAAAPDVMGYGGHARLSNQFVGVWMAAFGAI
                     AMSECLRAVQWANLVLGVWLVAAPFLLEYPDERACGSLVVGIAAISLALVRAPLRDRF
                     GGGWTSLWRQAER"
     gene            complement(63961..64524)
                     /locus_tag="PK224_05525"
     CDS             complement(63961..64524)
                     /locus_tag="PK224_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRB15227.1"
                     /translation="MTGTDALRPPGWTYNPSSWSERLWLIGVAILGFLISGYLGLYQW
                     GILDSVWEPWFGRGSETVLHSPLSRVLPVPDAILGAMAYGIDAVAGAIGGTTRWKTMP
                     WMVVLFGLAVGPLGLVSVLLVTAQPLLLHAWCTLCLLSAVISVVMIGPAMDEVLASLQ
                     VLKRAKEQRKAVWTTFWKGEGKAVWSG"
     gene            complement(64521..65606)
                     /locus_tag="PK224_05530"
     CDS             complement(64521..65606)
                     /locus_tag="PK224_05530"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB15228.1"
                     /translation="MQATTTKELVLVTGSSGLIGARTADRLSAEFRVAGLDRPGEPHP
                     PSSVENIPCDLTSDDSVVQALTMVKGRFGSDIAAVVHLAAYFDFSGEPSSKYSEVTVE
                     GTRRLLRALHTLGFRVARFIFSSTMLVHKPCEPGEAITEEWPLDPRWPYPQSKVETER
                     MLEKERGNYPTAVLRIAGVYDDDCDSIPLAHQIERINERHITSKMFPGHISHGQSFLH
                     LEDLVDAIVRVVERRRSLPDQAVMLLGEPEPLTYDELQHRFGRLLHGEEWDTVRIPKM
                     VAKTGAWLQGVTPLLEDPFIKPWMIDFADDHYALDVSRAKEWLDWEPRHSLRDTLPKM
                     IDALKRNPVAWYRAHQLSLPAELEETQ"
     gene            65977..66828
                     /locus_tag="PK224_05535"
     CDS             65977..66828
                     /locus_tag="PK224_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15229.1"
                     /translation="MKCITRSEYPYIGIRMMICFGLIGMWGALLPMAESAEAGQRISP
                     GEGAQLRDDAEKLLKGNRSVLGKVLAITSDQIKVDIGEVQPRFLPLKQAQQKGFPGVA
                     EGDDLIVVLNAQNLLVDYHPLDGQASAHTIIRGEIAQNLPVGQEQVVIKSAGKEMSFT
                     IRSQARSKVAAIPVGVAAVFLIDETNQIADVGLSGQQTSKQADRLTDSMPPIKGAHKQ
                     IDGTVTSPLRANHITVRTANGSEKPFEVRELLHKKLAALRKGDAVILLVDTDNKVIDV
                     AVPPPGR"
     gene            complement(66849..68234)
                     /locus_tag="PK224_05540"
     CDS             complement(66849..68234)
                     /locus_tag="PK224_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB15230.1"
                     /translation="MSTSHARILLVDSNADAAQELRDILSHEGYEVSSARDPKSARRY
                     FEQGSADLILTDLQLPQLDGLTFLTEMRTRFPNMPVVLMTANGSLRTAVEGLKAGAFD
                     YLTKPFIVDEVRCVARRALEHTSPPSESPTASDLQPEDASPFGSLVGSSPRMVHVYKA
                     IARVAQTDSTVLLQGESGTGKELIARAIHVSGPRSSGPFVTVDGGALADSLLESELFG
                     HERGSFTGAVSSRKGLLEKAHSGTCFLDEVADLSPALQAKLLRVIQEKEIRRVGSSAS
                     TSLDVRIIAASKLPLEPLVRAGTFREDLYYRMNVVTIDIPPLRERPEDIPALAQRFLN
                     LYGAIKIPPVKEISSEAMELLSRYWWPGNVRELEHAIERAVALSPHAVIYPEDLPPVV
                     KNAAPESLARAQGWVTLENLERDHILRVLDSHRHDVGRSAVILGIHRKTLLRKLRQYG
                     MATAAPAHGSL"
     gene            68901..69455
                     /locus_tag="PK224_05545"
     CDS             68901..69455
                     /locus_tag="PK224_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15231.1"
                     /translation="MKHLSLYALMVVVAAGISLPCEALEFTAERLSRTGQEVHHARIF
                     YRDHMWRLEYNEPGAVSATIVRADRNQVWHLIPSIHHFRTESYGSDYALHLTIRLDNE
                     TSREFIGTQTLDGHPTTLYEVVATGPGEQKETYYQWVATDMNFPLKLVKKDGDWMVEY
                     RDVKLGRLADSFFQLPHRYVPMEP"
     gene            complement(70130..70477)
                     /locus_tag="PK224_05550"
     CDS             complement(70130..70477)
                     /locus_tag="PK224_05550"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HRB15232.1"
                     /translation="MAQARNKADIEYAVMLAILNFHTEFMKSNYAHVRVQMSPDEIDV
                     TLTRQGSVPAEMRLARSEEGCALLRQVHEAMFVSCEDILRERIEPVVDRKVREMVTTL
                     DPLSGQSRIAIRF"
     gene            complement(71164..72570)
                     /locus_tag="PK224_05555"
     CDS             complement(71164..72570)
                     /locus_tag="PK224_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB15233.1"
                     /translation="MAWHLLLLEDEASVREAFALRLRDQGYVVQTAESGDEALGLLRS
                     FEPDVLVVDLVMPNFSGLEVLARVKQLSPALPVIVLTARGTVKDAVEAMRLGAFDFVA
                     KSIDMDDLLHTLRRATEFLTLQRQVQLHSGQDAARYALDRVIAKSPVTRSFLAQVQEL
                     IKNDRVTVLLQGETGTGKQYMGRVIHYNSPRAGRPCVEVDCPSIPRDLFESELFGHEK
                     GSFTGATGRKCGLIEMAEGGTVFFDEIADLPLPLQAKLLRVLEERTIRRVGSAVNIPI
                     DVRVMAATNRNLKEAVAKGEFREDLYFRLNVVTLTVPPLRERREDIIPLAEQFLNRSA
                     LALKKPVRTLGESAQALLRGYHLPGNVRELSNLIERAVLFCAGTTLEASNFPIDVQGA
                     LSIPVAASLDAYSVEADGSDSGDVHISFRVGAQSLADLEDRIIEEVLARSGGNKTLAA
                     KQLGVTRWMLDRRRKPRV"
     gene            complement(72572..74545)
                     /locus_tag="PK224_05560"
     CDS             complement(72572..74545)
                     /locus_tag="PK224_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15234.1"
                     /translation="MASTHRSRSKASRPVRSEIAAAEVLAWLSDCIHGGLCFVCQGLL
                     IFENSQFAELVESASPRVGVGEHALRKRLLDDAIAWNTRALGARKTVEYTVEAQDGRP
                     LYYACRFNVVPYRGERGVLMVVEDVTERVLLAQDASHVARFQSALARIGTLAVSGLSA
                     QALMNEAVQETATALEVELCKILVPRERDGHLYIMAGIGLRSDLIGKLTIEGGTHSQA
                     GYAIRERIPVVVDDFRRETRFSASKLLTEHGAVAGMCVPMLVEDRVYGVMTAHSKRVR
                     KFNQKEQEFLCTMANTIGTVLERRRHEETQMDFYHRLFLSAQDGVMMTDTTGRIMEWN
                     PALERMTGWTREEALGRRPAILKSGKHPPEFYERLWASIRAGHPFVERFVNRRKDGSE
                     FLVWESVSPVKASDGTTQYFMAILTDLSEREQMLEALRHTEQVKLVGQLAGGMLHEVR
                     NPLIGLGSLATHLADQASLPQEARDRCRLIAREAARIDELLESHLGQLRPRPFDIAVC
                     ELPAVLDDTLTLLRPNLIKHHILVKSSLAPDLHAVDASRAHILQVCLNIAMNAIDAMP
                     GGGNLNVTLSPDVRRVPGVLMRFADSGKGIAGEDLTHIYEPFFTNGKAKGVGLGLTIT
                     RDIVERHHGQLIIQSPPGSGAVVDVWLPLKHEA"
     gene            complement(74662..74985)
                     /locus_tag="PK224_05565"
     CDS             complement(74662..74985)
                     /locus_tag="PK224_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRB15235.1"
                     /translation="MLTLHPMKEIRIVVEGEHLKLVTNLLDRVGATGYTIINNVSGKG
                     HHGFHEGHLLFDDTSSQVIIFTVVPEERIEPILAGLGPLFHKHSGAMFVSDVAVTRRD
                     HFVVP"
     gene            complement(75064..78414)
                     /locus_tag="PK224_05570"
     CDS             complement(75064..78414)
                     /locus_tag="PK224_05570"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HRB15236.1"
                     /translation="MAVESRTFTDAERMELRSHVELAGEAISSYWPMRTFIHHNPLHG
                     LEDLPFAQAVKRGEQLLGGKGYLPSASYRRYFEQGRIRQEDLTRVLAPLASERRVLFA
                     GREISHLDLLRLSMVHGLSEAAEAYADPSVPVEDRLDQLALWLATAVGQTRTSQADTL
                     IPWETVDLLSHETLSAWCDRTLGTALVADINEQMIKWCSAFLDEGEASWTMPDRQHTF
                     YRAWKRLARYDASLRLLGVVDASQKIDALGDRPEEAVLDSLAVMGIPKSAWESYFAWH
                     LSAMPGWTGYIKWRSEEQHHPWQARYPVDLVKYLAVRLFYEREFVALHCRQSLDIAGH
                     YDAIRASIEQAPYAHWLRRQLTEGRLPASAAAEAREWRRLHRTAQPDEWNGIGRQVYE
                     QTAGWRASEAVKRDVRRLWALSAAMGVDPEQISETQPTDVSTILNWLDGFPSGQQSQR
                     WLDAFEARHRRDIVGQLRSVAAQLRDAGDRVSSGGSSRPLAQMVFCIDIRSEVLRRHL
                     EQLGGYETLGVAGFFGIPVKYQAFGGEHPVTHSPVLLKPKNHIREIPRSYHGAAASRH
                     RLFARLNKAGHELLHDLKENVITPYVMVEALGWFFSVPFFGKTLCPLWYHRLTSWVKG
                     LWLPPLATTLTIDKLTREEAEEMVAAEQRASIRAALRREFPALGSAITPALIETIRLG
                     ALENQDEQSRGEVANALGIAPSEADALHERLRRELALTPRGMSTRMQRITLHGFSVAE
                     QAYGVEAALRLMGFTSGFSRLVVMCSHGSTSDNNPYESALDCGACGGNSGLPNARAFA
                     SMANNPAVRKVLESRGVKIPGDTHFVAALHDTTRNDVRIVDLEDVPPTHRKDLVRLLE
                     DLKEAGTQAALERGLALERPGAKPTRRDPQQRASRRSVDWAQVRPEWGLSKNNLLIIG
                     RRELTRPLDLQGRSFLHSYDYRQDSSGKLLETIMTAPLIVAQWINMEHYFSTVDNEVY
                     GSGSKVYHNIVGRVGVMSGATSDLRLGLPAQTVLDGPVPYHEPMRLLTVIEAPRALVE
                     SVIAKHPGLERLFQNEWVSLVVCEPNEAAFYHYDIMQGWRLVSEELELRQELEGSSGR
                     RPDAGESGAQPGSGGPVREGEEPAASRP"
     gene            complement(78453..>79685)
                     /locus_tag="PK224_05575"
     CDS             complement(78453..>79685)
                     /locus_tag="PK224_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRB15237.1"
                     /translation="LARLLVCNPDSRAARDAGQKTFWVQSFGDAAFAVALLVLYGSYG
                     TWDMTDLFARLQTAPAPLTVWPGLQWDCDVPLVVTLLLVLSVMTKSAQFPFHFWLIGT
                     IEAPTPVSALMHAGIVNAGGFLVNRLAPLFGLAPTTLHLMFVIGGITALIGATAMLTQ
                     TSVKRRLVYSTMGQMGYMVMECGLGAFALAVFHLCAHGLFKATLFLNSGSVIHKARTE
                     FKLPPVGRADEAHTFSPMTWTTGLVATLVLPLVILLVVHGVVSIPLQDAQGAVIFLFF
                     GWVTTSQAMFTLYRLHTGASWAVSLTMLGTLAFIGLTYLWAGEAFTHLLFPGPGIAES
                     YLRAAEWNRSFFELVIGLAAILIVGAWGMIYGKAKGVSLLMPAWTETLRVRAYVMFLN
                     GLYVEDLVRMIGRVAFRR"
BASE COUNT        16743 a        24837 c        22194 g        15911 t
ORIGIN      
        1 ccaatgatag ccggcgatca ccgttcgcgc ttcatcgcct gacgctcttg caagcgcttg
       61 ctcttccatg cgcgaacccg ggaggacgat aaactggtcg gccgcaagcg tcagccatcg
      121 ttcaatatca tgctgacttg catccggcag ctgtgccacc aacttactga gccgttgcgc
      181 ttccgcgtca aaaatcgctt cgggagtgaa ctcgaggtgt tcccacggct ccgtgctgga
      241 cacaaacgcg atgggcagac ccggcatcag gtccgcgctg aagtagccgg gacttgcgag
      301 gctttcgaca tgttctgccc cgcgatcgcg gtccactcga taggacacac cgggactcaa
      361 cagcgggtcg gctacaaaga ctccagcgtg aggccgcagg cacattttga gcgacggggc
      421 attttcgcac agagacattt cgtaacgccc gtcgagcacc gtgagcggaa agggaggctt
      481 cctcgcgtca tggagcggcg catccagcat gcgaaaggtc acgaacgggc ggagatgcaa
      541 ccggaccgga tcaccctcca gcaaccggta ttccacatag accgagttgt gaccgtacgg
      601 catgatgacg cgcttttcca gccgcctgcc cttgaatgag cagcgccaga caggagtttg
      661 ccactcgcgg gtgaacgcac tgatgacctt cgtcaggtcg ctctccaagc gtccatcttc
      721 aaactcgatg ccgctgagga gcacggactc tccctccacc acgacttctt cgtccaggcg
      781 gggaatcatg actgtccgcc cccaaggcga gggaagatcg ggcacgaaca tcccgtggta
      841 acggcgagtc ggggcgccga gcaatgtccc ggaggcatag ccgccgagac cattcgtcac
      901 cagccattct ctggagagca aggcatcgtt gtcctgaagc tgctccagcg gcacagagag
      961 agtcatatta tcgacttgca ccatggctct acccttccta tcaaacctcg ctcctccgag
     1021 actgacctcc cgttcttaac cttggacgga atacaacgct gccgattcac caggaatgac
     1081 ccatccggtt tcggtcaagg gctcgatgac tccgggtcct ccatattccg gcgcatcgct
     1141 cgaccagagc aggtgccagg ttcgcccatc aggcggggca agcaatggtt caggagcggg
     1201 acgatagtcg caatccgccc ccagattgat gagcagcagc cgatcatcgc cttgtttacc
     1261 ggcataccgc aggacgaacg ctgccggtcc gatcacggcg ccatccagcc ggtcacgagc
     1321 ctgttgcgcg atgaccggat cttcccgccg gaggcgaagc agatctcgat gcaatcggac
     1381 gtgctcggca tgccgctcgc gctccgacca atcgattttg gaacgctgga acacggcagg
     1441 gtcgcgagga tcagcgacgg ccgcctgagc ccccgcggag gcataactcg gaaactgagc
     1501 gaggaactgc tttctccctt ggtggatctg ccgggccaat tcgccagctg gaaagtcggc
     1561 gaagaacaaa aagggagcgg atgccccgaa ctcctgcccc atgaataaca tcggtgtttc
     1621 gggagccagc aggaggagcg ccgtcaaggc gcgatgcaga ccgggaccgg tcttctggtg
     1681 caatcggtct ccccgcaact gattggccac ctggtcatgg ttctgaagaa aaaatacaaa
     1741 ctgctccgcc ggctcggcac gaacggccgt tccacgcggg ccatcttgcc attgatagcg
     1801 ttgtccctgg taaagaaacg cccgctttac acaggaaatc agctcctgcg gcgtgccccg
     1861 atagtcggtg taatagcctt cgcttcggcc cgtcgcggcc acgcgcgcgc tgtggtgaaa
     1921 atcttcactc cagaccgcat cgagccccca tccccgttcg accgtcggtc gaatcgtgca
     1981 gacccactgc gcctcacatt cggcgaccag gatgatcgat cgtgctcctg ccgcctttct
     2041 ggcagcctga gacaattctg cgatcacgtg cagaggcccc tcatcatgaa aggcgtggac
     2101 ggcatccagc ctgaggccat cgagatgaaa ctcctcgatc cagtaacagg cattctggat
     2161 aaaaaactcc cgcacctctt tagagccggg tccatcgaaa ttgatcgctt gcccccactc
     2221 gttgggatat cgatcggtga ggtattcgtc gctgaacgcg ggcagatagt ttccatccgg
     2281 tcccaaatgg ttgtacacaa cgtcgagaat cacacccagg cctcgcagat gcgcctcgtc
     2341 cacaaaccgc ttcaaggcat cgggatcacc gtacacatgc gcaggcgcat agaggccgac
     2401 cccgtcatag ccccaattcc atcgaccggg aaactccgcc accggcatga tctcgataac
     2461 ggtgatgccg agatccttca gggtatcgag acgcgtgacg gctgcatcga atgtcccttc
     2521 cggggtaaac gcgccgatat gcagctcata gatcacctgc ccatgcatcg tcaggcctgg
     2581 ccacccgcga tccctccaag caaacgtgtc cgggtcgacg atcagcgaag gcctatgggg
     2641 tccatccggt tgatatcgag aacagggatc cggatagagg ctgtttccat ccaagcgata
     2701 gcggtagctc atgccggccc tggcctccgg gatgacaccg gtccagtatc catcggctcc
     2761 ccttgtgagg ggatgaacgg ttccatgggg ctcgagaacg acatccagct gcgcaggctt
     2821 gggtgcccaa cagcggaatt gcacgccatc ctggatgacc tgagccccaa gccgtttacg
     2881 gccggccgac ggggcttgat tgccatgtgg ttgctccggc acgacggtag ccgacccgct
     2941 catgatgcaa ttcctttggc agccccgact gagagcagga tgcggttgcg caaaaatctc
     3001 aataccgctg cctcctccct cgggagctgc tgggacgagc caggcactcc gttgtggata
     3061 ttcgggccaa ggcttttcag taacgtgccg tccaaatagg cgtgaacaat ttccggatgg
     3121 atataacatt ttcgacagac ggctttggta ttgcccagct gccgcgccac ggcgtcgatt
     3181 gccgccgcca cattccgctt cgccttggcc aacgagtcgc actgatcctc atattttctg
     3241 agcgcgcgtg ccgccaacag ggtcccggcc caggtgcgaa aatccttcgc cgtgaaggct
     3301 tctccggcga tgcggcgcac atattcattc acgtcgccgg acgtgatgca acgccgtctc
     3361 ccctcctcat cgacgtactg aaataattcg taccctggga ggtcctgact ctgtttgacg
     3421 atgcgagcca cgcgcccgtc ggtgatgtcc acgctgtggc ggatcccact tttgccccga
     3481 aattcaaatc ttaacgtgga acccttcacc ttcacgtgac gatccctcag cgtcgtcaag
     3541 ccgaaggact cgttcgtgcg cgcatactct tcattgccga tacgaattaa cgtcgcttca
     3601 agcaatcgga ccacggtcgc cagaattttg tcccgtgaca ggcccgaacg gcgcaggtcg
     3661 gcggccacgc gtctgcgtat tcgcggcagt gcgagggcga acgtgagcat ccgatcatat
     3721 ttgctctcat ttcgaatctg gttccagcgg ggatgatacc ggtactgttt gcggcctctc
     3781 gcatcgcgac cggtcgcctg caaatgtcct tgcggatcca tgcaaatcca cacatccgtc
     3841 cacgccggtg gaatcaccag agagcgaatg cgtttcagac aagtctgatc ccggatgacg
     3901 gtgccgtccg ggcggacgta gcgggcattc cttccccgtc ccttgcgggc aatgcccggc
     3961 gagcggtcaa gaacgtagcg caggcccgcg accttggccg atcgagccga attctcatcg
     4021 gatggatgtg agtaagcctt gatcgctgtg aggccgcagg ctgatttcat tggtcgattc
     4081 atgccgtttt gcgaccggcc tgtcgctcac gtttcggcaa atgccgcttt ggttgtccgg
     4141 actgttgcac gcttcgcttg aggagtgcca tcaaatccac gactttggca cggccaggct
     4201 tttccccggc ttcgcctttg tccggcgctg tcaccacttc agtttttccg gccttgatcc
     4261 gcttattgat caatcgcatg agatcgtcgt gataggtgtc cttgtattga accggtttcc
     4321 agctatccga catttcctcc accagtttgg tcgccatctc tatctctcgt ggcgtcacgc
     4381 ctgtcgcctt taagctcttc gccggaatct cgagatcctt gatcggccgg agttcgttgg
     4441 catagcgcag cgtgttcaac accatcatct cactccaggg aatcagcgcg gcgatatatt
     4501 gcctcgtcct gatcacaacc gtggcaatgg caaccttccc tgtcttggtc aacgtttcgc
     4561 gcaggagcgc ataactcttt tctccccgtt tatcaggcgc caggtaatac ggcgtctcaa
     4621 aggacgtggg cgaaatatct tccgcgcgca cgaagctcag gatatcgatg gtctgcgtgg
     4681 cctcgacatt cgcgcgtcga aaatcttcgt ccgtcagaac gacgtaacgg ctattttcat
     4741 attcataccc tttgacgatg tggtcccacg ccacttcctt gcccgtctcc ttgttgtagc
     4801 gcttgaatcc gaccggtttc atgtcgcgac ggtcgaggag cgtcaaatca aagctgttcc
     4861 ggttttcagc cgaatacagc acaacaggaa tgttgacgag cccaaaactg atggatcctt
     4921 tccagagcga tcgcggcatg actcaccgtt tatgagatac caggaaacac tcccgctctg
     4981 aggtcaggac gcagatcatg cagagctgca gacaattgac acaggccact ggttggccac
     5041 agccctcaca gaccacaggc atcgtttcgt taacctgtcg gcagccgtct cgccggcagg
     5101 atggatcaat gcagcggcta cagtcgtaag gacaaatcct catggtcgtt gctcgatcat
     5161 tccagggatc tcaaaatacc atgcgggttc ctgtcgccaa ctcggataac ccctagcccg
     5221 gactattcat gaatccccgc tccggcgtcc gatcctctcg cccggcgagg ctgttctcgt
     5281 tcggcctcct cgacggactg ccagtacgcc tgcgtcggcc ttacgtgcga gcaaccccgg
     5341 cgggcttccg tctcgaacgc ctcgcgacgg cattcgtgaa taatccgggc tagccgccgc
     5401 tcctgcgtcg agagcgggca ctcgaaaact aggcccctgc ctagctggat tttccgctct
     5461 ctcctctagt gtcacatgta tacgccaccc accccgtcaa tgggcctcta cgttaggaga
     5521 tcacaatgaa cgacaagtct ggacaaagtg cttcaatctg gatgtcacag gacgtcccgg
     5581 ccatgccccc gatagtctca ggagctcacg ccgacgtgtg cattgtaggg gctggaatcg
     5641 ccgggctcac gacggcctac tgcctgatga aggagggcaa atccatcatc ctgttggacg
     5701 ccggacagcc tggcggaggc atgactcaac ggacgacggc gcacctgtcg aacgccctcg
     5761 acgatcgcta cgtggaaata gaacgtctcc atggcgagac cggcgcacaa ctggcggctc
     5821 atagccacac ggccgctata gattggatcg acagggttcg attcgaagaa cggatcgagt
     5881 gcgactttac gagactggac ggttacctct tcgttccttc tgataatgcc cgacacctta
     5941 tcgaggagga atggcaggcc gccagacggg caggagtcga cggtgtcgag cgactcgacc
     6001 ggttgccggg gaatatgttc ggaaccggac cctgcctgcg gtttcccagg caggcccaat
     6061 ttcacccgtt gaaatacgtg tcgggcctgg tcaaggccat tctagccggc ggggggcgcg
     6121 tcttcagcaa tgcgcatgtt accagcgtcg ccagcgatac gcaggtcaaa ctggaaacga
     6181 gccaggggag cgtggtgacg gccgatgccg tcgtcatcgc caccaataca cccatcaaca
     6241 atctggtgac cattcatacc aaacaagcgg cttacatcag ttatgtgatc ggagcacgca
     6301 taccggccgg atcgatcgaa ccggcattat tttgggatac gctcgacccg tatcattatg
     6361 tgcgaattca tcggcaggta tcggcgcggg agggagagga ttccgtcctc atcgtgggcg
     6421 gcgaggacca caaggcagga caggcggacg acggagaagc ccgctatgct cgcctggagg
     6481 cgtgggcgcg cgagcgtttt cccatgatga atgccgtcgt atttcgatgg tccggacaag
     6541 tcatggagtc ggtggacggc ctggccttca tcggccggaa cccgggcgat acggacaata
     6601 tttacatcgc aaccggagac tccggtatgg gcatgaccca tggcacaatt gccggactgc
     6661 tgatcaccga tctcattatg aaacgcgagt cgccttggtc ctcgttgtat gatccgtcgc
     6721 gccggccggt gcgcgccgca ggcgaattta tccgtgaatc actgaacgtc gcggctcaat
     6781 ataccgactg ggtaaccgga ggggatgttg aaaccgaggc acagattcca catgcacagg
     6841 gagcggtact gagggatgga ctgacaaaga tcgcgatcta ccgcgatgcc cagggagaac
     6901 tccacaagtg ttcggccgtg tgcccgcatc tgggctgcat cgtggcctgg aaccatgcag
     6961 aaaatacgtg ggactgtccc tgtcacggct caaggttcga caaattcgga aaagttctca
     7021 atggtcctgc gacgagcgac ctgagcccac tcaagcagga agatacttag gcaacgtcat
     7081 ggccgagagt ctgttttgca ccgacgaatc atgccccgca ccttttcagc cttctgatgg
     7141 cgtggatggg agcagccccc tcgtggaacc tccctaggac agttcccagc tctccaatga
     7201 ttcggaccag gatagggtgg gcccatgccc aaacagatac ttccttcatg tgcaaaggag
     7261 aagaaactga atgcgctatc tagcgttggc cgccgactat gacggcacat tggcaaatgc
     7321 agggactgtc ggcccggaca ccatccaagc catcgaacgc ctggtggcct cgggtcgaaa
     7381 actcattctg gtcaccggac gggtcttgcc ggaaatctta gagatttttc cccagatatc
     7441 tctgtgcgaa cgtgtcgtgg cggaaaatgg cgcagtcctc taccggccag ccaccaaaga
     7501 aatcacgcta cttgcacctc ctcctccatc ggcattcctg gaggaagtcc aacgtcgcaa
     7561 ggtagagcat ctcaccctcg ggaattcgat cgttgcgacc cgggttccgt atgagacggt
     7621 catcctggac atcattcgtg atctcggtct cgaactgcgc attatcttca acaagggcgc
     7681 agtgatggtg ctatcccatg gcatcaataa ggccaccggg ctgaccgcag cgctcaagca
     7741 gttggaattg tcccctcata acattgctgc ggtcggagac ggagaaaacg atcatgccat
     7801 gctgacatac tccgaatatg ccgtggccgt ggagaatgcc gttccgatgt tgaaggaaac
     7861 agcagatcgc accaccgtcg gcgatcatgg ccacggcgtc atcgagttga tcaatgagct
     7921 ggtcgagaac gatctggcgg tagcggatcg atcggtagcg cgacatcgga ttgcgctcgg
     7981 cacacaggag aacggaggcg acatcacctt tcatccggcg cgacaaaacc tcttgcttgc
     8041 cggcacgtcc ggcagcggaa agtctacgct ggcgacgggg ctactcgagc gccttggcga
     8101 acgaggttat caattgtgtg tgatcgatcc ggaaggggac tacgagaatt ttccacaggc
     8161 gattgtcctc ggaactgcac aggatggtcc cagtcacgcc gaaattctca cggcattggc
     8221 caatccgaac aaccatgtgg tcgtgaacct tgtgggactt cctctacagg accggccctc
     8281 attttttctc accctgttgc ccaaacttca ggaactccga tcgaaatcgg gtcgccccca
     8341 ttggatgctg gtcgatgaaa cgcatcatct gttgcccgtc gatgggaacc ccacgacacc
     8401 ggggctgatg aaggatttag ccggcatgat ttacgtcacc gttcatcccg accacatcga
     8461 acattcgatc ctcaagaccg tggatattgt gttcgccctg ggcaaatcgc cggacgagac
     8521 gttgaagcaa tattgtgctg cgatccaaca gccggctccg gccgcgactg ccgcccggct
     8581 tcaaccgggt cgagcgatta tgtggaatcg cgcctccggg gagacgcctt tcgtgcttga
     8641 gatcgcacca agcacgatcg aacgccgccg gcatcgacgg aaatatgcgg aaggtgaatt
     8701 acctccggag caaagttttt actttcgagg gccggccgga cagctcaatc ttcgggctca
     8761 caatttactg ctctttatgc agctcggcga aggtgtcgac caggcaacat ggatacatca
     8821 cctccgctct caggattatt cgacctggat caagcaggtt atcaaggatg aggcgcttgc
     8881 gcagcgcgta cacgacgtgg aacagcaggc tcatctgccc gcagaagaga gccggcaact
     8941 gatccgcagc gcgatcgaag aacggtacac ggtccctgca ggcggggatg aacatacctc
     9001 ttgacctggg acactatcgc ccgcaccgcc aggggatcgc ctagtaccga agttcccggt
     9061 cctgcggcat cgtaaaagca gccgcggaca tgtattcgag gttaatgtcc acgatacgga
     9121 aggaaatcgg cgacacgaga acagccccgc ttccttcctt tttaatccat tatcgcaaga
     9181 ggagacgata ccatgccgat ccttttatgg cttctgggag tgccgataag tttgattctg
     9241 gtgctgtggt tgttcggtgt cgtcgggttc tgatcacgaa ccgggggaca ggccgtgcag
     9301 gtcgtcgatg agacgttgga tagacgttac cgccttacgc ctctgttgta cggtcagcag
     9361 gcggtcggct tccaacaaca gaactgtcag ggatgcccgc aactcgccga ccagctcacg
     9421 atagccggcc ggcgcggtct ggtccggctg gacgaatatc attcgtaact cccgactcat
     9481 gtcctcgaac gatgcaggac gccgaagcaa cgtcaagagt tcctgctgac gctgccggcg
     9541 atgttgccag atcacaggtt gggtgtcggg aagggccctc gtgaaggggc tgattcgcgc
     9601 cacctgctcg acgctcaagg gacccaacca atcctcggcc gtggcgagca ttttccttgt
     9661 gcgctcttcg agacgtttcg aagttgatcc ttgcagcgtg cgcacgaact tgtgttcctc
     9721 tttacgaaac acgtcctcca gatttcgaac ctgccgctcg ctgacggtca ttaataatgc
     9781 gctgccgtcc ggcacagctc gctcgaacaa atcctctcga aaccggtcgt aggacgcgta
     9841 catccactcc aggtcttcac gggtaagccc tcggctcacc cgttgctgca actctttcaa
     9901 gaattgttcg tactgtggga tcgcctcggt cctgtgccgt gcgaggagcg gttttaagcg
     9961 ggccgtgaca tcccggcgct ggcctgccgt gagatccaag tagtgatcca attgccagct
    10021 gatcatccaa tcggcatgcc gatatccaaa ggtgagcgcg caaccgctca gcagaacacc
    10081 catcgacagc aggcagagcg ccgcagttcg taccgtcccc cacatatgcg tccttttcat
    10141 cgttggccgg atctccgaag gatgggcaag agccgctctt gcttcgtcct tctccatcaa
    10201 taaccagtca ggcctcgcgc gcatactggg ggtctcccga gtacggtcgc gcaccaggcc
    10261 tcggtactgt gagtatcgaa tgcgatgacc tgcaaaatcc tgcaaggacg gcattcctgc
    10321 aacaccatgt tcatcttacc aaggaggaaa tcgatgaaga ccgcactgca ttcgctgctc
    10381 gcactggcgc ttgtttcact ggctgcctgc tcccactcac cgaccgcgtc gaaaacccgc
    10441 tcagagatgg aggcaggcac gaagaccggt atggtcaaga acatcgtcat caaggaaacg
    10501 atcgtccccg atgccttaac ggtacgggcg ggcgacgagg ttcgatggat caatcagaga
    10561 caagactccg tcaccgtgac gatcgatgaa ccgctggaac acaacgtctc ctgccggaac
    10621 ggattttcca aggcgatggg aatgggcgta gacaactcca cgaccttatc gagcaatgag
    10681 accgctggtc tctgttttag ccggatcggc accgtgaagt attccgtacg accggcggac
    10741 gaaaccaaga ccagtatggc taacacccat ggctcgatca ccgttcagta atgcgtgaat
    10801 agcaagccgc cgcccacacc atcacggtgt ggacggcggc tcccctcagt acaaccagtc
    10861 tcacgcttag atcacatttc acgtcatgag ggactggcga gtctaatttc tctggcccaa
    10921 tggcgatgca tctcgatacc cttcatgaag cgggctgcga tctgcttggg cgaagagtcc
    10981 ggccccagaa cgattcccga atcctgcgac cagtctttgg gcgtctgtct ggttctgctt
    11041 ccatgttccg cctccgggta ggccgccccg accgccgctt caaacaaggc aaccccctcc
    11101 cccgttgcgg caatcgcctt gcaatgcagg taggcgtcct gaacgaacgt tctcgctttg
    11161 ggggttcgca acaatgcgct gacgctttgt tcaccgcctg gaatgtagac cgcatcgaat
    11221 aacaccgatc ccgtcgtcaa caggctgaaa tcgatcttca gctcacttcc gttcgcagcc
    11281 ttgaggcttc ccgaacgggt cgccacaatc ttgccctgcc ctccggcggc gaccaaggcc
    11341 atctgcattc ccttgaccgc ctcgccatct actccatcgg caaccagaat cgccaccttt
    11401 ctggtttgaa tgccttcctt aggagccaac accatgctca gcgccgcaga cttgtccagc
    11461 gaagtcatcg cccgcttggg ttgaaagtcc ttcggatccc cgtcggcggg gatactcaaa
    11521 ttgagtagac cctcgatctt cttgaccggc atacccaagc cctccgcgac ccgtccagcc
    11581 aggcctttgt cgatctggga cagcatgccg accattcgtt cccgaatcgg gaggttctcg
    11641 accttgccta gttcaaactg caacgcctgc accaaatgct cttgctccgg ttcagactga
    11701 cttcgccaaa acagcgcagc ctgactgaaa tggtcgaaaa agctcttgct ccgttctcgc
    11761 actttgctgc cgggcagccg ctccgcataa ctcaggaacc cgcccatatc tgccttcgcc
    11821 tgcatgggac aaccaccccc caatgtgttc ggttggtaac tcaccggccc ggaattgatc
    11881 gtctgccgca tatggccgtc gcgttgattg ttatgcaacg gcgccagcgg tcgattgatg
    11941 ggaatctcat ggaagttcgg tccgcccagt cgaatgagtt gagtgtccgt atatgagaag
    12001 agtcgacctt gtaacagcgg atcgttggtg aaatcgatgc cgggaacgag gtggccgggg
    12061 tggaaggcca cctgttcggt ttcggcaaaa aaattatccg gattgcgatc gagtgtcatc
    12121 ttgccgattc gaaccaccgg caccagttcc tcgggaatga gcttggtcgg atccagaaga
    12181 tcgaattcga acttctgttc gtccttctcc tccacaatct gcaccccgag ctcccattcc
    12241 gggtactgcc ccgcctcgat cgcctcccac aaatcacgcc ggtgaaaatc gggatccaag
    12301 ccggaaattt tctgcgcctc atcccacaag agtgaatgta caccgaggag cggcttccag
    12361 tgaaatttca cgaaccgtcc gacgcccttg gcattcacga agcgaaaggt atgcaccccg
    12421 aagccttcca tcatgcggaa actccggggg atggcccgat ccgacatgac ccacatgatc
    12481 atgtgcatgg attccggcat cagggagata aagtcccaga acgtgtcatg cgccgaggcc
    12541 gcctgcggca tctcatgatg aggctccggt ttcacggcat ggaccagatc cgggaatttc
    12601 accgcatcct ggatgaagaa gaccgggatg ttgttgccca ccaggtcgta attcccttcc
    12661 tccgtgtaaa acttcacggc gaagccgcgc gcatcccgcg tcagatcact ggaacctcgg
    12721 gacccggcca cggtcgaaaa acgcacaaag accggggtct tgacctgcgg gtcctgtaaa
    12781 aattttgccc tcgtcagcga tgcgagtgac ttatacactt ggaaatagcc gtgcgcggcg
    12841 gcgcccctgg catgcaccac cctctcggga atacgctcat ggtcgaaatg cgtgattttt
    12901 tcccgtagga tgaaatcttc cagcaatgtc gggccgcgag cccccgcctt gaggctgttc
    12961 tgatcatcgt tgatgcgcag gccttgatcg gtggtcagaa agtgatcgcc ggacttctcc
    13021 cgcgcgctgt caagggcagc ggttttctca ttctcccgct tggaacgatc tttgggcatg
    13081 gcacagtcct cccctggtcg gccttcgccg gcatctaccg aattgttttc ctggatgggc
    13141 gggcgccggt ttttcgaccg gatgctttgg ccagcaacgt ctccttgcgc ttcatgacac
    13201 tggcctccaa cgattcccaa tcgatgtcgc actcttcggc tttcggaaac atttcgcctt
    13261 cttcttcctc gatatggtgc ttcaccagtt cacttaaaac gctgaacttg gcctgaaatt
    13321 tctcgtcgcc aggtttgagt ttcttcaatt cggcgatgag cacatggacg aggtgatgtt
    13381 cctcaatcgc ctcgttcatc atgtccgcct catccacctc cgctcgaatt gccggataaa
    13441 tcaaacgttc ttccaggtcg gcatgaattt ccagctcccg gatcgccgtt tttgcaatct
    13501 cgccccgctc ccgtccctcc gccgattcga aatcctcaaa cagcttcttg accttgtcgt
    13561 gatcctcttt cagcatttcg aaaaccgtcg aaacgaatcc tgccgctcgt gccatggtca
    13621 gctcctttcg aatgaagaga ttccttactg ccaggcgcaa cctatcacgc ggatccaacc
    13681 gggtgagact cgtggaagcc ctagttatcg acggagtgac gacccttgtg tacggactag
    13741 ggctactcct agtggaccgg ccaggggcgt cacgtatgct gctaaagaat ccgttcaaca
    13801 ccctcaaccc tggaggtgta gccatgacaa cgccacggtt cattcccatt tgctgcggtt
    13861 gcaacaatgt gcgagacgat tcacgacgtg atgagagaga accgggatgg ggaacgttgg
    13921 acgcctatct ccacacccat catctgacca gcggggacta ccggttgacc catacctact
    13981 gcccttcctg ctcggttcgt ttttcgaaac tgagcggtgg ccatgcggtc acgccggagg
    14041 ttgcggcaag ccgttcgctg tgaaacgtga acgggcagac gtaaaacgta tccaggagca
    14101 cttcgtccga cggattatga cgccacctgc gcgggaacag cccgggaaag tggcgacgtg
    14161 tcacgttttg cgccccacat tttacgcctc acggttcacg tctcaaggaa ggtaaatgtc
    14221 ggccttgacc ggcagatggt ccgaggccag tcggctccgg ggagtatcgt gcgcttccac
    14281 tgtgagtagc gcctgagcgg gaaagatcca gatccggtcc agcgccaaca ccggccatcg
    14341 agcgggaaag gtgaccggag cagccgggga accgaacacc cgacgcaacc cgcgcaagga
    14401 ttttccccat ccgtgccact cattcaggtc tcccatcaaa atagtgagat cacgccggct
    14461 gagtgacagg agttccaaca atcgatccgc ttgttgcggc cgttcgcgag gccacagtcc
    14521 caggtgggta ttgagcacgt gaaccgttcg gccgcagatt gtgagctcga catccagcgc
    14581 gcccctcggt tcgtgccgac ccactgagag atcccatcgc cgcacccgat ccaccgggta
    14641 ccggctcagc agcgcgttgc cataccccac accttcccgt gaccacacag gtccgccgat
    14701 ggctatcatc ccggttttct gcgccagcca cggcgcaata ggagcgtggt cctgccttgg
    14761 aaaatcaact tcctgcaacg caatgatgtc cgcgttgagt tcttgcgcga cacgcaggat
    14821 gcggccgggc tcatatcgcc cgtcccgtcc gatagcgcga tggatattgt aggaggctac
    14881 ccgcagacgc atcgtgctcc tattcacacg gcctcaggcg gccggcgcca cctgctgaac
    14941 gttgccgaca tccggtgggg tgtccctgac ctgtaaccgc cgcgacacgt accaggcgac
    15001 actccatgcc gcagccatga gcaggcctag cacgccaaag gtgacgggac tcggattgcg
    15061 cagggcttcc gccaaacggt cgacgaacgc cgacatcagc acaattccgg ggctcatgcc
    15121 gagcgccgtc ccaagcagaa aatcgcggag cgacagcgag gtcgctccgg caaccagatt
    15181 gaccatggaa aagggcgcga taggcaggag cctgacgatc agaatcgccc agaatcctct
    15241 ttgggcaatc cggcggctca aatgcgccac tcgacggccg gcaaaacgct gcactcgata
    15301 tctacccaag gcccgaccca ggccaaaaat ggtcacggcg cttaggagcg cgccggccag
    15361 ggcggatggc gcaccgacca gcgggccgaa cgccagaatc gtcaccacaa tcaacaccgt
    15421 gataggaacc gcgagaaaac cacccaccag gaaaccgccc agcatgacga gcgaaccggt
    15481 aggtccctgg ccgagggccc gcacggcatc gaccaggtcg ttcacattca tccactcact
    15541 cagagggctc caacgccacg cagccgccaa gaggcccagc aggagcagca ccgtatatcc
    15601 aaggaacacc cggcgcccca catgtgcccg ctcccgatgt tgaaccacgg cccccaacac
    15661 ctcatccgcg gccatgggac gttcggggtc gatcaggagt tgttccggga ttgaaatggc
    15721 ttcggcgggg ctcttgtcga agcatccatc ttccaggctt cgatcgccgc cccgcaggtg
    15781 atcgatcgca ggccccaggg aatccagccg ctggacatgc tcagccacca gctcaggctc
    15841 gacgccgaga tgttccccga tcaatccatt gcgcaatctc gcaatcaccg cctggagccg
    15901 ggaggttccg ccggattcga tcgcgagatt acactccgta tcgaatccca tcgaacgatt
    15961 cgacacattg gccgacccca ggcaaacaaa atcttcgtca acgatgagca acttgctgtg
    16021 caccgccacc atgtccctgc cctgcgtgcc cggcacggta ggcgcgtaga tgcgcagacg
    16081 tccataatgg tcggcgagtt ccagctcatg caggatacgg gaacggagcg catccatcgt
    16141 ctggcgttcc aaccatccgt cgcaactgtg acgaagaacc aacacgactt ccggaccatc
    16201 ctgctccatg agccgggccg ccagggcccg actgatcgca ttggacgtaa aatactgcga
    16261 ctcgatataa atagagcggc gagcgcgctt gattccttcc agataggcca gttcgatctc
    16321 ccggacctca ggttgccccg cataggcagg ctgggtgcgc atgataccga cgccgcacgg
    16381 ttccacgtcc ggcacgaggg cgaccggcca gagctcctcc actggtcgtt gaatggcaag
    16441 gggaagtcgc cgtcccgtcg cgttaaacca tcggtcacga gccaactcac ccaaggcagc
    16501 ggccgcctca cccgacacca tcatctgaac atcgtgaaac ggcgcatagg gttgcccgtc
    16561 cgcgtcgacc cgaaggggat cctggcaagc atgcgcaggg gtatcccatc gggatttcgt
    16621 caaatccagc cctccgacaa aagcaatcgt gtcgtcgatg accacaagct tctggtgatg
    16681 cgaggcgccg ataggatgct gcccatccac ctgataggag agccgacggt ggccgctcca
    16741 gcccgtctgg gccgtcggca tccattcacg ctccagagcg tagatcatgg cgaagtccca
    16801 attgagcaca tggactctca gctttcgctt acgccgtagg agcgccgaca caaactcccc
    16861 gagtttcgac ggaaattcgg acggctctgc cggacccctg actaattcga tctgcgtatc
    16921 gagatcccat ccgacaatca tgatggaatg tttggcgtgc aatgccacat cccgaaacgt
    16981 cttgaaatac gcttccccat cgatcaagaa tccgcatcga tcgcttcgtt ccacacgcca
    17041 acaagtgcgg cctcgcacta aaaacgacgg cggtgtctgt tcctgcgcct gacatgcctt
    17101 caaaacttcg gctcgctcca tgcaacaccc tcccgcatcc acggtcacag tcgttccacg
    17161 cgcagtcggt ctcacaggcg atgcgcgcag tcactttaac tcagaccacg caggatgccc
    17221 agctggtcat acccctagct gttcgatcgg ctccattcga gcagctatcg ggatgtgtcg
    17281 agaggtttcc ctagtacctc ttgaatcgtg gaacgttaga atgaacttct caatctgaac
    17341 tggaggacgg cctatgacga tcgcggcatc aaaccattcc atcgcgcagc accggttcga
    17401 atgtattcaa gcctgtttgg attgcgctcg catgtgcaac acctgcgggg acgatatgat
    17461 cggcatgtct catgaagggg gagaggcaaa tttgatgaca cgatgcatca ggctctgccg
    17521 ggattgttcc gatatctgcc tccttgcagc ccagtggatg ggccgcactt cggcctttgc
    17581 tgtccagctt tgcgcgttgt gcgccgaaat ttgtgaggtc tgtgcgggag tctgtgagca
    17641 acatgcgccg catcatgccc tgtgtggaga atgcgcgaca gcctgccgcc gttgcgctgt
    17701 agaatgccat aagatggtga tggaaacagt cccgagttcc tccggtggta cggcctagcc
    17761 gccccgcatt tgatcgtcag tcgccagtaa ccggggaaag gaggtcatca tgttacgcac
    17821 catcatcgga gtcggcctgg tgagtggagc cctgctgacg gggtggagtc cttcatggaa
    17881 cgaggtacca gcccccgttc cgcatcccgg tcccgggccg accgttccac cgaaaccgtc
    17941 cgaccccgtt ccgcttccac ccgatccgaa accggtgctg ccgccccatc cagcaaatac
    18001 tccgcatgct tatctcatgg cggatccggc gacagatcca cagaacaccg tgtccggggt
    18061 gttggaaaaa gtggatttga tcgcgaaaca gggactgctc aagaccgatc ttggtcggga
    18121 agtggctttt caaatcccaa aaccggaact ctttctcaat ttgtccgtgg gtcaacgtgt
    18181 caccctcaaa ctcgatgatc gccaacaggc tgtccgggtg atggacacca cggcgcctga
    18241 gattcccccc cctttccccg caaaataagg tacggtgtca tacacgccgg tgtcgaacta
    18301 ggggtttcac tagtggggcg acctggccca aggtgctact acccatgcgc attgtgcaat
    18361 gttgcgtcat gggccccaca atccgtccat gaccaggcac atagagtcat catccagccc
    18421 gatgcgattg agcggcagat gaattcaagc gacagcgtca gttggttgac cggcagtgag
    18481 atgggtggcc ggatccggac gttcgattgg cgccggaccc cgcttgggcc gatcgaagat
    18541 tggcctgccg cactcgtgtc catcctcggt gtctgtctga ccgcgcaata tcccatggcc
    18601 atctactggg ggtcggaagg ctggctcctc tacaacgatg cctggcggcc tatccttggc
    18661 gataagcacc cttgggcgct cggtcgcgcg gcccacgaag tgtggcctga gttgtgggac
    18721 accatcagtc cccttctcca tagcgtccag actaccggtc aagccgtctg gcgaggcgac
    18781 gaactcttgc ccatgcaacg gttcggctat accgaggaat gttatttcga ctatagcttc
    18841 aaccccattc gcggacaaaa cggcgcagtc gagggcatcc tgaatatcgt ccaggaaacc
    18901 acctatcggg tcttaaacga ccggcgcatg cgcctcctgc gtgaactggc ctcccggtca
    18961 gggttcgccc atagccagga agatgcctgc aatctggcca tcgaagcgct cgctaccgat
    19021 caagccgacg ttccctttgc gctcctctac catatcgacc gggatcgtcg gcacgcacac
    19081 ttaatcgctt ccacgggact gcctccggaa catccggctc ggcaacagac cgtgagcctc
    19141 acgccggagg agcctgattc cgggtggccg ctgtcagccc ctctccaaga aggcgttccg
    19201 gttatcgtcg acgacgcagg cgaccgcttc gggccactgc ccggcgggtc ctggtcggaa
    19261 ccgaccgggc aagccctcct cctgccgctc agcacggtac gttgggatgg ccgtgccgtc
    19321 atcctggcgg cagggatcaa cccccgccgg ccgctggacg acgactaccg ctcgttcttc
    19381 accatggtcg agtctcacat cgcaggagcc ctgactaatg cggaggccta ctcttccgag
    19441 aaacgccggg ccgaggccct gaccgaactc gatcgcgcca agaccgcgtt cttcagcaac
    19501 gtcagtcacg aatttcgaac gccgctcacc ctcatgctcg ggccgctgga ggaggaactt
    19561 cgtgagcgcc cccatgcgcc ccgcctcgag gtggcgcatc gcaacagtct tcggctgctg
    19621 aaactagtca acacgctgct ggaattttcg cgactggaag caggccgcgt cgaggcgcgc
    19681 tatgaaccga ccgacctggc aggctatagc gccgaactcg caagccagtt ccgatcggct
    19741 gtggagcagg cagggttggt attccggatc gactgtccgc cattgcctga aacgatctat
    19801 gtcgatcgtg acatgtggga gaagatcgtt ctgaacctgc tcagcaacgc cttcaaattc
    19861 actttccagg gccagatcgc gctctcactt catgcacaga caggcggggt ggaactacac
    19921 gtccaggata ccggtgtcgg cattccgccc gaagccctcc cgaggctctt cgaacgtttc
    19981 tatcgcgtgc aggatatgcg cagccggact tatgaaggga gcggtatcgg cttggcgctg
    20041 gttcaggagt tggtccaact tcacggagga accgtccggg tcaacagcca ggtggaccaa
    20101 ggcagcacct ttgtcgttac gctgccgttc ggaaacgccc atctcccgcc ggataaggtc
    20161 gtcccgcacg gccgaccaac cacacccacg acgggagtcg ggccctatct ggaggaagcg
    20221 ctccactggc tgcccgaacc gcaacacgcg acgaagccgg cggacgggag gcctgatcct
    20281 gccgaagccc ctctctccac aacgagcgcg gagacggccc gacccagggt tctcgtggtc
    20341 gatgacaatg cggatatgcg gcaatacatc acccgtctct tggcggatcg gtatcacgtc
    20401 attgccatgc cggaaggcat gtccgccctg atcgaggccc gccggcaacc gccggatttg
    20461 attctcagcg atatcatgat gccgcaactg gacggcttcg gactgcttca agccctgcgc
    20521 gacgaccccg ccctcaagac cactcctgtc attttgctct ccgcgcgcgc aggcgaagag
    20581 tccagaatcg aagggctggg gcgtggcgcg gacgactatc tcgtcaaacc tttcggcgcc
    20641 cgtgaattac tcgcgcgagt cgccacccat ctcgacatgg cgcgcgtccg acgtgaggcg
    20701 caaagggaga ttgcacacag cgaggcaagg tttgaggcca tcgtcagcca agcgacggcc
    20761 gccgtcgcgc agaccgatct caccgggcga ttcctgttcg tgaatcaacg cttctgcgag
    20821 ttgctcggat acagtgcatc cgaactgctc gggatgaata tgcagcaggt caccgatccg
    20881 gatgacctgc cggccaacct cgtgctcttt catcggttga ccgccggcgg tccggacttc
    20941 gtgattgaga agcgataccg gcgaaaggac ggctccgcgg tctggaccag agtcagtgtg
    21001 ggcggggttc gggaagacgg cgaactgcgc catatcctgg ccgtggggct cgacatcacc
    21061 gaccgcaaac gtcaggaagc ggaactcctg gccctcaccc accgacaatc cctcttgtac
    21121 gacctggccg atgtcgtgaa ccgggcggag gccttgaccg gcctctacga gaaagccatc
    21181 gacacgatca tcgaggccct gcatgcggac cgcgcctcga ttcttctgtt cgacgacgag
    21241 ggcgtgatgc gcttcgaagc ctggcgcggc ctgtcgcaag cctatcgaga ggcagtcgag
    21301 ggccattccc cctggattcc cgaccagcag gatcccccgg ccatcttcat tcccgatgtc
    21361 gccgcatccg acctccacac gtctttgcga tccgttattc agagcgaagg tgtctccgcg
    21421 ctgggattta tcccgctgac gtatggcggc cggttactcg gcaaattcat gatgtatttc
    21481 gaccggcccc acgtgatgag cgagcaggag ataggcttgg cccagacgat agccaggacc
    21541 ctggccctcg ggatcgaacg ggcccgggcc ggcgagttat tgcggtccaa tgaggaacga
    21601 cttcgtttgg ccatggcagc cggctcgatg ggcgcgtggg acgcggacct ctcgaccgga
    21661 gcaaccgatt gggacgccaa acaatacgag ttgttcgata gaccgaaaga caagcccctc
    21721 acccatgcgg agcagttcta cgaatccatc caccccgacg atgtgccccg cgtgagggaa
    21781 gcggccgcgc aagccgagct gacgggctca ttctctcaag aatttcgagt gatcgcgcag
    21841 gacggctcga tccgctggct ctccgggcac gggacggcgg tgaagaacga ccagggacag
    21901 gccgtccgga tggtcgggat caattacgac attacgcgac gaaagcacgt ggaggaagcc
    21961 ctccagcggt ccgcctccga cctggagcag cgcgtcgtgg aacgcaccct cgaactgtcc
    22021 cgttccaggg aacagcttcg ggcgctcgcc accgaattga atctcgccgg acagcgggaa
    22081 cggcaacacc tggcgactga gttgcacgat tatctggctc aactcctggc actgagccga
    22141 atccgtctgg cccaggccaa gcagcagccc atgacgacgg ccttgggaaa gatcctgagc
    22201 gagttgcagg acgtgatgga tcaggccctg acctacaccc gcaccctcgt ggcacagctc
    22261 agcccgccgc ttctgaaaga attcggattg ccgatggccc tgaagtggct ggcggaacaa
    22321 atgatacagc gagacctcaa agtgtccctg gaactcggga cggagcacct cgccctgccg
    22381 gaagaccagg ccatgctgct cttccaatcg gtacgcgaac tgctcatgaa tatcgtgaaa
    22441 catgcctctg ccaaacaggc gcggatcacg gtcagggagg aggagggatg gctacatatt
    22501 accgtcgcgg accagggccg aggcttctcc cctccggact ccattcctga aacaggctcc
    22561 acaggcatcc cgggctttgg actcttcagc atacgcgagc gcatgctcgc gcttggcggc
    22621 cggtttgaat tgcaatccca tcccggtcaa ggtaccgaag ccacgttgac cgcaccgctt
    22681 acaccgggca aggcgcgggc caaacaaccg gcagaagtgg acgggactgt cccgagcatc
    22741 gcccccctca atacgaaaca ccaggcggct gagcgggagg gtccggtcgg caaacgcgag
    22801 actgtcgagc gtcccatccg cgtgttgatc gccgacgacc atgccatggt gctacagggc
    22861 ttgtgcggac ttctggccgc ctatgggaat atccaggtcg tgggagaggc agccgatggt
    22921 gaagaggccg tcaccctgac gatacagcta cagcccgacg tcgtcctcat ggacgtcaac
    22981 atgccgcaaa tggacggaat cgaagccacc aggcggatca agcaagtcct tccggcgacg
    23041 atcgtcatcg ggctctccgt ccaaaacgct gcccatgtgg gacgcgtgat gagagaagca
    23101 ggggctgcgg cctttctcaa taaagaggcc gccgtggaag acttgtatca aaccatccag
    23161 gtggcccgga aagaactgct acaggtccca tgatacgatt ccaacaagct cccaatcccc
    23221 tgctgtccgg cgcgatcgtc ttctctctgg caaccgtctt cgtgctggat ttttacacgc
    23281 cgctcggcat caccgtgtgg gtgctctacc tgatcccggt cgtgctcgcc tatctcttct
    23341 ggtttcctgt cacgccggtg gccgtggcag gagcggtgac gatccttact atcgcaggcc
    23401 tcttcctcag cgcccctggc atcgccccct ccgtttcacg catcaatcgc ctggttggaa
    23461 tcattacctc ctgggcgctg gcggccttcg gcgcgcattt catccgcaac cgcctcgtca
    23521 tgcgcagaga agaatggata cgggcaggcc aaacgctcct cagcgaacgg atgatgggag
    23581 agttggcgct gaacagcctg ggcgaaaacc tgttgcattg cctcgcaaaa tacctgggag
    23641 ctgaggccgg cgccattttc atcgacgacg ggcgcggctt tcaccgcacg gcgacctatg
    23701 gcgtcccgga aggcactgcc atgccggaac ggttcgcagc gggcgacgga ctcctgggcc
    23761 aggcgctaca gcacaaacac acggtcgtgg tgcatgactt gccggaaggg tatttcacca
    23821 tcggctccgc tttgggacgg ggtgccccgc gccacctgat gatcgcgccg ttgaccgtcg
    23881 atggacgcgt caacagcgtt ttggaactcg gattctttac tcgcgtgacg gaatccgacc
    23941 gggagtttgt cgatcgcgtg gcggaatcga tcgccgtcgc cgtccggtcg gcccgatacc
    24001 ggactcgcct tcaggatctc ctggaggaga cccagcggca agccgaagag ttgcaagtgc
    24061 aaagtgagga actccgcgtc gccaacgaag aactcgagga acagagccgg gcgctgaagg
    24121 aatcgcacag ccgcctcgaa cagcaacagg cggaactgga acagaccaac tcccaattgg
    24181 aagagcaggc tcaaatcctt gaaattcaga aggaagatct caatcaatcg acggcgattc
    24241 tcgaatcgca aaccagacag ttagagcggg ccagtcgcta taagtccgag gttttggcca
    24301 acatgtcgca cgaaatgcgg acccccctga actcgacgct gatcctgacg aaactgttgg
    24361 ccgacaatcc acagggcaac ctcaccgccg accagatcaa gtccctcaca acgatcgaat
    24421 cctccggcca cgacctgctg gcgctgatcg acgacgtcct ggacctggcc aaggtggagg
    24481 ccggacgtat cgacctcacc ccgcaaccag tgccgatccc gcggctgctc gacagcttga
    24541 gatcgatatt caaaccactc gcggaacaac gggggctccg gctcaccatg caacagcggc
    24601 cgggcacgcc cgactcgttc gaaaccgact cgcaacggct cgaacaggtg ttgaagaatc
    24661 tcctgtcgaa cgccatcaaa ttcaccgagg tcggcgaagt gtcgctggaa gtatcccgcc
    24721 tctcggacgg gcgtctggcg ttcacggttc aagacagcgg catcggcatt ccgcaagacc
    24781 agcatgagct gattttcgaa cccttctgcc aggccgacgg caccaccagc cgaaaatacg
    24841 gcggaaccgg actcggactc tccatctcgc gtgaattcgt tcgattgctg gggggagaaa
    24901 ttcatctgac gagtaccccg ggccagggca gcagcttcac cgtgctcctc ccccaacgct
    24961 tatcgtccgc gccatttcct gcgcctgccc gttccctcca tccacaatcg ccgcccaaac
    25021 cgatgaggtc cggcgacatt ccccccatcc ctgcaatccc gtctgagagc ctggtggcga
    25081 cgacacacat cgaggatgat cgggaacagc tggccgccaa caggcgggtg attttgatcg
    25141 tcgaggatga cgaacccttt tcgaagattc ttgcggacct ggcccatgaa ctgaattttc
    25201 aggtcctggt cgccacgacg gccgccgatg ccctggcgct ggcctcgcgc tacctgccga
    25261 gcgcgatcat cctcgatatc ggacttcccg accactccgg gctctccgtc atcgatcgcc
    25321 tgaaagccga tccccggacg cgacatattc cgattcacgt cgtctcgggt cacgattatg
    25381 agaggaccgc gctctcgtta ggcgccgtcg gctacatgct caaaccggtg aaacgggagc
    25441 agctggtgga ggccttccgg caattcgaga ccaggctgac ggataaaccg cggcgagtgc
    25501 tgatcgtgga agacgacccc gcccagcggg agagcctgcg tctactgctc ggttcggacg
    25561 aagtcgaaac gatcggcgcg gacagcgcgg cggaatgtct cgcccatctc gccgagcgaa
    25621 ccttcgattg catggtgctc gacctcacgc tgcccgatgc gtcgggcttt tcgttgctcg
    25681 aaaccctcag ccgggaggag cacctcgcgt ttccaccggt gatcgtctat acgggccggg
    25741 agctttctcc cgatgaggag cagcggctgc gcaaatattc gagttcgatc atcatcaagg
    25801 gcgcgaaatc tccggaacgg ttgttggacg aagtgaccct gtttctgcac caggtcgtga
    25861 cggcgcttcc accggagcaa cagcggatgt tggagaaggc gcgcagccgg aacgccgtgc
    25921 tggaaagccg gcgcattctc atcgtcgaag acgacgtacg gaacattttt gccttgagcg
    25981 cggtcctgga accgcatggg gcgaagatcg agatcgcgcg gaacggccgc gagtcgctgc
    26041 gcatcctgga ggaatcgctt caacacgacg accggctgat cgatctggtg ctgatggata
    26101 tcatgatgcc tgaaatggac ggcctcacgg ccatgcgcgc catccgcgaa cggcccgaat
    26161 gggacacact tcccatcatc gccctgacgg ccaaggcgat gaaaaacgac caggagcagg
    26221 cgctggccgc cggggcgaac gactacatgg ccaaaccgct cgatgtcgat caattgctgt
    26281 cgctcgtgcg cgtatggatg ccccgatgac ctccgacacg gcgatgaaga cagaagatat
    26341 cgaactgcag ctgttccttg aggccgtgta tcaacagtat cactatgatt ttcgaggcta
    26401 ctccaaggcc tcgatcaaac ggcggctcct gctggcacgc gaacgcatcg ggtgcaccac
    26461 cttttccggg ctacaagata ggatgctgca cgatcatgcg gtgcttcctc agttgctgga
    26521 ctacctgacc gtgcaggtca gcgatatgtt ccgcgatccc ggctattttc gcgcgctacg
    26581 ggaacagatc cttccccacc tcaagaccta tccctcgttg aaggtctggg tggccggctg
    26641 cagttcgggt gaagaattgt actccctcgc cattctgttc cgtgaggaag ggctggagag
    26701 taagaccatg ttttatgcca ccgacatcaa tgccggcgcg ctcgccaagg cagaggccgg
    26761 ggtctacgat ctccagcgga tcgcgctctt tacgaggaac caccgtctgg ccggtgggaa
    26821 aggttcgcta tcggactact acagggccgc gtacggggcg gccaggttcg ataaaacgct
    26881 ccggcggcgg acggtctttt ccgaccatag tctggtctcg gattcggtct ttgccgaggt
    26941 gcatctggtg tcctgccgga acgtgctgat ttatttcgat cggccgatgc aagaccgcgc
    27001 gatcggcctg ttcaaggagg ctctcgtgag aaaagggttt ctcggtatcg gcgccagaga
    27061 gagcctgcgc ttcaacgccc acgtcaagac gtttacggag ttactccccg gcgagcggat
    27121 ttatcagaaa cgaggcgatg agtgacggcg ctccccctgc atgaatgcct gcgagaacca
    27181 cgcgccatcg tcatcggcgc gtccgccggc gcggtcgctg cgctttctcg cctcctgccc
    27241 ccgctggcgg cagactttcc gctggcaatc ctggtgacag tacacgtgcc cgccgacaag
    27301 cccaacagta ttccggcctt gttgcagacc aagtgccgga ttgcgatcaa ggaagcagag
    27361 gataaggagc ccatcgtgcc cggaaccgtc tacttcgctc cgcccgacta tcatcttctc
    27421 gtggagcagg atcgacgcct gtcgctgtcc aatgaggaac cggtgaactt ctcccgcccg
    27481 tctgtcgatg tgctcttcga atcggctgcg gacgcgtacg gcaaggactt actggcgatc
    27541 attctcagcg gcgcgaacga ggacggcgcc aggggggctc gcgcagtccg cgaagccggc
    27601 gggatcgtcg tggtgcaaag cccggattct gccgaggccc gcatgatgcc ggaatccgcc
    27661 ttgagggcct gtccgaaggc gcgagcgatg aacctgcata cactcgcgga gctgttgtgc
    27721 gcctgtggag gcaccgtcca gtgctgaccg attcaccggc taaattcctc ttagtcgacg
    27781 accatgagcc caacctcgtg gccctgagcg ccgtcctgtc ccgtgacgac gtcgaaatgt
    27841 tacgcgccag gagcgggagg gaagcgcttg agctgttgct ccaacacgac atcgccctgg
    27901 ctgtcatcga tgtccagatg ccgatcatgg acggctttga actggcggaa ctcatgcgtg
    27961 gctcccggcg cacccagcat gtgccgatta tttttctgac ggcagggcct caagacaacc
    28021 tgcgccgctt tcgcggctac caggccggcg cggtggattt tctctacaag cccattgagc
    28081 cggacgtgtt gcgcagcaag accgggattt tcctggacct gtaccggcag cgggaggagc
    28141 tggcccggca gcgtgacaca ttcatcacgc tcgctgaaga aaaggcccgc ctcctgcggg
    28201 aacgcgacga ggccaatcgt cggctgcgcg aaagcgaaat gcgcttcagg acattggccg
    28261 acagcgcgcc ggtgatcatc tggatgaacg gaccgtccgg ctgcgaattc gtcaaccaga
    28321 cctgcctcga tttcctgggc atcgaccacc tgggccagat cgacatattc gactgggctc
    28381 gccacgtcca tccggacgat cgcgaccagg cggccgcggt ctaccggcag gcggtcgagg
    28441 gccgaacccg tttcgaggcc tctttgcgct gtcgtcgacg ggacggcacc tatcgatgga
    28501 ttcgttctgt cggcatgccg aatctctctg ccgacggagg cgtcctgggt tatatcgggg
    28561 cgagtttcga cgtcaccgaa agcaaggaag ccgaagaacg cctgcaacga tggagcgtgg
    28621 accttgaacg ggccgtcagc caaaaaacat cggagttgct gcaatcgcag gagcagcttc
    28681 gtgcgttggc gacggagttg aacctgaccg agcaacggga acgcaaacga ctggccacgg
    28741 agctccacga ctacctggcc caactgcttg tggtcgtgcg gatgaaatta cggcagacca
    28801 ttcatctggt cacgggggaa cggatccccg aactgttgaa agacgccgac cacgccctga
    28861 ctcaatccct ggactacacc cggtcgctcg tcgcagagtt gacgcccccc accctcaagg
    28921 aattcggcct gctccaggcg ctgacctggc tggccacaca gatgcatcgc cacgggctca
    28981 ccgtcaccgt gcgacaggac gcagcgcctc tgccgctgcc ggaagaccag gcggtgctcc
    29041 tgtttcaatc cgtgcgcgaa ctgttgttca acgtcttgaa acatgccaag gcagcggagg
    29101 cgaccgtcgt gctcgggttg agagaggatg gtcaattgga ggtggtggtc gccgatgacg
    29161 gctgcgggtt tgtcactgcg ccgaagaatc gacccggaac caccgccaac cgcttcgggt
    29221 tgttcaggat tcaagagcgc atggccgcga tgggtgggca attggtcatc gaatccgtcc
    29281 tgggtggcgg gacacgcgcc acactcacga cgccctattg gaacagccgg gagaaaccgg
    29341 aggacgacga caccctgacc ccggcggaat cacgggcacc ctcgctgcgg acccggtcca
    29401 cagacgtgga gcacctttcc cattctcatc atcccacgtc gaaaatcggc attctgctgg
    29461 tagacgacca cgccatggtc cgccagggat tgcggagcgt gctggaacat tatgacgacg
    29521 tcgcggtggt gggcgaagct tcaaccggcc gcgaagccat cgagttcgtg gaacggctgc
    29581 ggcctgcggt gatcgtcatg gatatcaata tgccggagac caacgggatc gacgcgacgg
    29641 tcgaaatcaa ggcacggcat ccggacgtgg ccgtcatcgg actctctgtc cacaacactg
    29701 ccgaggcacg gaaggccatg ctgcaggccg gcgccaccac cttgctctcg aaagagtcgg
    29761 ccgtcgacga actctacgcc gcgatccgac aggcgctggc agacgaggga gtgccacggt
    29821 gagtcgccgg tcaccacatg acgatggagc gccagcgatg ccccaaatcc gcggtcggga
    29881 cggacattcg tacgatgcct gcgtcgatgg tcaccctggc atgttcgatt tgggcatccg
    29941 tcgggatcgg cacgtatcgt tgatgcgacc cgctgctgag aatgatgccc tgccgggcga
    30001 actcaacggc aatctcctcg gcactggcca tctccggcat cgctcgaaag tctgcataga
    30061 cattgaacgt gccatcggac tcggacacct caatcaaggg gccttgctga acagcgcgag
    30121 aggaaccgag tgaaccggca gttgtctgtg cggatgcttg ccgggagtca ttcaatcgca
    30181 ttccatcgat ctcgccaccc aatgtctgct tcatacttgc tccaatcgtt atgccatcat
    30241 gttgaaggag tctcgctgtg tcctgccggt accttacggc atactgtcgg gcaaaccact
    30301 aggattttcc cgcggtccag acctggaaga cgctagcggt ttgcctagtt gttcacccgg
    30361 gcccgacatc ctaatatcac gtccgcgccc ggatcggtct ttgttcgcgc acaatgaggc
    30421 acactgagat gcattcgaca gacggagaca accccactgc ttccgagatt ccgcttcaga
    30481 ttgatctcct cctgtcgcgt ctggctgcga tcgtggagtc gtccgatgac gccatcatct
    30541 ccaaagacct caatggctgc atcatgagtt ggaatcaggg ggcgcaacgc ctcttcggat
    30601 acacagcaca ggaaatgatc ggacagccgg tgcatcgcct gatcccccaa gaccgactcg
    30661 atgaagaacc cagaattctg gaacgcctcc gcctggggca acggatcgac cattacgaaa
    30721 cggtgcggca gcgcaaagat ggaacgatga tcgacgtgtc gctttcggtg tcgccgatca
    30781 aggaccgggg aggccgaatt gtcggcgcct caaaagtcgc ccgggagatt tcggaacgaa
    30841 aacagggtga gcgtgacctg gcaaatctgg tcgaagccct tccggtcgcg gtctacacga
    30901 ccgatgccga gggacgaatc acgcgctaca atcaggcggc tgtggatctc tggggatcca
    30961 gacccgtgct cggcaccacc agatggcgcg gctcatggcg ggtctactgg ccagacggtc
    31021 gccccatgcc gcttgcagcc tgtccgctcg cccagtgcct caggctcaat cgcccgatac
    31081 gcggacttga aacgatcatt gaacgcccgg acggaacccg ggtcccggtc gcaccccacc
    31141 ccacccccct tcgcaacgcg gccggagaat tggtcggcgg ggtggacgtg ctcatggacc
    31201 tcacggaacg gaaacgcgcg gaacgccaac tgcaggagtc cgcggctgag ctcgaacgtc
    31261 gcatcaagga acgcacgcat gaactcgtcc tgtctcagga acggctgcgg gcactggcct
    31321 cggaactgac gttgactgaa caacgcgagc gccgacggct cgccgttgaa ttgcacgact
    31381 atcttgcgca gctcgtcgtg gccagccggc tcaggctttc gcaagccatc cccaaggttc
    31441 aggatgaaac cgcatccgcc gcgctgacga acgtggacgg gatgctggat caggccttga
    31501 cctacacccg atcgctcgtg gccgaattga gccctcacat cctctaccaa ttcggcctct
    31561 ccaaatcgct ggtctggctg ggagagcgga tgaagcaaca cgcgcttgaa gtcacggtca
    31621 aaacgggtcc gacgtcgttt accctgcccg acgaccaggc cgttctcttg tttcaatcgg
    31681 tccgcgaatt gctgtttaac atcgtcaaac atgccaagac agatcgcgct gtcatcacga
    31741 tcaacgtcga tgagcaacgg gagctgtgga tctgcgtcga ggatgaaggc gtcggattcg
    31801 acgtgacgga actgaccgac ggcgaggaca gccggggaaa attcggcctg ctcagcatcc
    31861 gggaacggat ggaaacgctg ggaggagaat gcgaactctc cgccgcgccc ggcgccggca
    31921 ccgtcgcgat cctccgcctg ccgctctccg ggagcgccac cgcctcgcct tccacgccac
    31981 ccaccacggt tccttcgcat acgccggccg ccgcgtccaa agaccctgcc cgacggatca
    32041 gaatcgtgct tgtcgacgac cacgccatgg tccggcaagg actgcgcagc atactggatg
    32101 gctatgcgaa cctgatcgtt gtcggcgagg gagccgacgg ccaggacgcc gtcgtgatgg
    32161 tccgttcgct tcgcccggat gtcgtgatta tggacgtgaa cctgccgctc atcgacggca
    32221 tcgaagccac ccgcatcgtg tcgcgcgaat atccctctat cgccgtcatc ggcatctcgg
    32281 tgaggaacga cccccaggtg catgtcgcca tgactgaagc cggagcggtg gcattcctcc
    32341 cgaaagaatc cgcagccagc caactctacg aaattatcct ccgccattgc cctgcgatcg
    32401 ctatatgaga tttcgcccct gacggaggag gactagggta tcccccagtt ctcaatcgtc
    32461 ttccgacgcg gtatgctgtt tgtaccccgt gtgtaccgcc gcttgccttg ctgttccgac
    32521 ctgaccgact ctcgtgcctg cactgggtat gtccttcgat gaagagcctg cgaaaggcct
    32581 tcatcgcacg atgtgacggc atcaaccgaa ggtatgttgt tctcgcatga cctcgatgct
    32641 cctcacattg cagcgtgtgg tcctatcggc cgccggactg gctatcgcgt tcccagccct
    32701 cacattgctc tgctgggcct acgaccattc gatcctgtac catctgcata cgtctttccg
    32761 cacactgaaa cccacgacca tgttggcggt cctcttctgc ggcctatccc tctggttgca
    32821 agctgaagag ggcgccgtca cgaccgcacg gcggtatgtt gcacagggcg ccgcgatcgt
    32881 cgcctgcctg cttgccagcc tgatcctgtc ggaacacctc cttgctctcg acctgggaat
    32941 cgacctctgg cgcattgccc tcccggaaat ctccagcgat gcacatcctg ggcgcacggc
    33001 tcctgccaca gccgtcgcca ttctgatgct gggactgaca ctgttgtggc ttgataccaa
    33061 accgttcgcg acatacttct tcgatcagtc tgccgcactg ataggaggaa cgatcggctg
    33121 catcgggctg gtcgggtatt tgtacggggt tcagagcctt tacaaagtgg ggaccttctc
    33181 atccacctcc gtctatagcg ccctcctgtt ggtgacattg ggcctgggaa ttctctgcgc
    33241 gcgaccggcg aggggcctga tggccaccct caccagcgag aagctcgggg gggtgatgtt
    33301 gcgacgtctc ctgcccttcg ccgtcatgct gccgatactc gccggatgga ttcgcattgc
    33361 agcccagcgg tcggggaagt acgacttcaa tttcggcgtg gcactggctg tggccgctgt
    33421 gatgctgatc atgctggcgt tcctctggct tctggcagga agcctgaacc acaccgacgg
    33481 ccagaagaaa cacatcatgc aaattctcca acagcgtgaa gcccggcatc gcctggcgtt
    33541 gagagccggc cgcatgggca catttcacca ggacctcgac gacgagtcgc tggtgttctc
    33601 ccctgaactt gaggccattt tcggcctccc tcccatgagc ttcgccagca ccttcgcggc
    33661 ctttcaacaa ctgattcatc ccgatgatcg acaacgggtg cagcagaccg ttgccgaggc
    33721 ggttgaaaac aggaccgcct acgaccttga gtgccggatc cttcgcacga gccctcccgg
    33781 tgagagttgg attgcggtga ccgggcaggt gctccccgac tcgaccgggc atccgcgaca
    33841 aatcaccggc gtcatgttcg acattaccga acggaaacga tcggaaaccg cgcttcgaca
    33901 gagcgaagaa caactgcggg tcgtcaccga tgcgttgccc ggcctcatcg cctatgtgga
    33961 ccgcgatgaa cgataccgtt ttgtgaacgc cggatatgaa cggcattttg gacggtctcg
    34021 tgagcagatc gtcggaagct ctgtcaaaga actcctgcaa caggattatc cgcaagtaca
    34081 acgtttcatc gagcgggtac tggagggcga gcagatctca ttcgaaacta ccagtcttgg
    34141 tgagaccgac gaacagacgc tcctggcgac ctatgtcccg gaacggacag ccgggggaac
    34201 agtcgaaggc ttctatgtcc tcatcaccaa catcacagac cggaaacgga cagagcaggc
    34261 cctgagggaa agcgaagaac ggtttcgaca cctttttgag caggcttccg acggtatcgt
    34321 catggcggat atgtcgggcc attacctgga tgtgaatagt cgggcttgtc acatgctggg
    34381 atattctcgc gaagaactgc tcggcatggg cgtgaccgac ctgctcgatc cgcatgacta
    34441 caaacgcctg cgggagatca aggccgagtt gctggaagga caccgtcatt ccggtgaatg
    34501 gaagttgcgc aggagggacg gagcgacact gtccgtggag atcagtgcca aactccagcc
    34561 cgacagacgc tggcatgcca tcgtccgcga catcaccgaa cgcaaacggg ccgaggccgg
    34621 gctgcgggag agcgaagccc atttccgcat gttggcggat gccactccgg tgctcgtttg
    34681 gatggcgggc accgacaccc gctgcacctg gctgaatcga tcgtggcttg agtacacagg
    34741 ccgtcccctg gaagacgagc tcggcagcgg atggacatcc ggcattcatc cggacgatca
    34801 ggccgagtac ctgcgagtat acaaagcgca cttctcgcgg cacgagccgt ttgaaatgga
    34861 ataccgcctc cgccgtcaag acgggaccta tgggtggatt ctcgaccggg gggtgccgct
    34921 cctgacgcag aacggagaat tcagcgggta cctcggctca gccatcgaca tcaccgaccg
    34981 aaagcatgcc gaagagcagt tgcagcaatg gacgggcgag ttggagaagc gggtggatga
    35041 acgaacacaa gcgttggtgc gttcacagga acgtcttcgt gccctcgcct ccgacctcag
    35101 tgcgaccgaa caacaagaac gccggcggct ggcgacggaa cttcacgatt atctggcgca
    35161 attgctcgtg gtcgggcgca tgaaactggg ccaggcgaaa ccgcacgtgc aagattccaa
    35221 gaccctgcaa ctcctgagcg aggcggatga cgtcctcacg caatcactca actatactcg
    35281 ttttctcgtg gcggaactga gcccccaggt gctctatcaa ttcggcttac ctgcggcgct
    35341 caaatggctg ggtggccaaa tgaaaaccca tgggctgacc gtcatcgtcc attgtgaccg
    35401 cgatcatctc cccatggacg aggaaagcgc ggtcattctc taccaaagtg ttcgggaatt
    35461 gctgttcaat gtcgtcaagc atgcacggac tacggaggcc gccatttccc tctcgtgccg
    35521 caccgatgac atcgtcacca tcaccgtctc cgaccaggga tgcggattca atctttcgac
    35581 aatgacggaa accgatcggg ctctccccgg ccggttcggc ctgttcaatg tgcaagagcg
    35641 cgttgaggcg atcggcgggc gtcttaattt ggattccacg gaaggcaagg gcacgaccgt
    35701 cacgctgaca gccccgattg acggcaactc ccaatccgag ccgggggcag tgtcgtctcg
    35761 cgccaaacca ctcagcgcct cgcccgttcc tgcctgcgag cgtctccgta tcctgctcgt
    35821 cgatgaccac gaaatggttc gacaaggcct acggagcgtg ctggacaact acgaggacct
    35881 ggaggtcatc ggcgaagcgg gtgacggcga gtcggcgatc agcatcgccg cggcgctgaa
    35941 gccggatgtc gtggtgatgg atgtgaatat gccgcggatc gacgggattg aagcgacccg
    36001 ccgcatcgtc gcagcgcaac ccgacatcgt cgtgatcgga ttgtcggttc agaacgaacg
    36061 ccatgttgaa gaggccatga ccagggccgg ggcggcggtc tttgtgacaa aagaacgcgc
    36121 cgccggccag ctctatgaag caatcgtgcg cacagtccgc acgagaaagt aggagcggcc
    36181 tttagacggc gggagggcgc ttgcccatca ccatgccgat caccgccaga atcagaaata
    36241 cgacaaacag cacataggcg atatgggtcg cggtccccgc cacaccgctc actcccaata
    36301 cccccgcgat cagcccgatg actaaaaatg tcacagccca gctcaacatg atggcacctc
    36361 atctgaaatg gtggacgata cgcttgcacc gcatggaacc actatgaatg caatggatgc
    36421 ttcgtgacgc agcccttgtg cccgcaggca caggctccgc tgggagccga cgagctgcgg
    36481 caggcgtcgg aacaaaactc ccgccccttc tcagcagggc aggaacatcc gggatgcgca
    36541 caggatttct gtccactggc gggatctggc atcatgacct ccttccagac gaccgaggcc
    36601 gtctttcatg acggagttgc ggaaccgaac gaacacccat aagcaccacg ggtcccaccg
    36661 agaaggcgag cggcacagct cgatacgttt ctaccgccgg tccgctcatc agaaaaacgg
    36721 gccgctcatc gcaggcttgg atcagaacca gagatggact cccattccga tccattcgac
    36781 tttttgtgtg aacagttgtc cgtccggact ggggccggaa aagtattcgg ctaagacctg
    36841 gagctttcta tcgccgatcc gggcattctc aaactgcaac cctcccatga tcgattgggc
    36901 gacattccaa ttcgtgcgag cgttggcttg aaaatccgca tagacgaccg gccgcacctt
    36961 gccgccgaag aacaaccagg gactcttgaa ctcgactcct ccctggctcg tttcccggcc
    37021 gagggtgctg ggatcctttc tgacgatggt accgacccca ccgtagactc tcagccatgt
    37081 cgtcaggtca tacgacacct tgagatcgat ctcttcataa ctcagattga ttctggcgac
    37141 ctgggcattg ttgaggagaa actcatcgcc taaatgcgaa ctctggtgac gaacccggaa
    37201 gaacccggag agtttatccg cgcgataact ggccaataac ccgaccatat agtcggcatt
    37261 gatcaaatcg atggaccgtt tctccgtatc gaagacgccg aacacccccg cctgcatccc
    37321 caattcccat tgtccgtcga acggagcggc gttccggtag aaggcaaacg attcaccgaa
    37381 cgcgctggca aattccttcc cggatgaaag attgtgatag gccgcgccga agtgtggcca
    37441 cctcaggtcc gcgtgcaggg gatccacgag caatccccgc ggcaggaact ttgattctgc
    37501 ttccggcgtc accgcccgcg ctgcggtgcc ccccgacgac ggcaccggct ctttccccgc
    37561 ctgaacatcg gccctcacca cgccgggtat ctgctctaac gctctgacga tcttggcagg
    37621 gtcttctcct cccaatcgtt tgacctgtac ggtgacggcg ccacggtcta cttctatcac
    37681 cgctccagaa aggtgaaact cgtgctccag ggtagcggcc gcatagcccg cgatatactc
    37741 atcggagccg gcggccgccg cccccgccgc aacgaacgcg caggtcacac acgcgaggag
    37801 caccaccttc catggaggga tcattccctt cggactcctc tcagagtcat gcccgatcca
    37861 gaccgtctcc gcctgttatc cagaatcaca gatcgaacca tagccacacc ctagaaatgg
    37921 aagttgaggc cgaccgtgac catgtgacca ttgtcctgga acttcttgtc cacgatgttc
    37981 tggtctcgag actcaatctt ttccagccag atgtagcgat aggtgctatc gatcgagacg
    38041 tgcttattga agaagaattg cagaccgcca ccggcatgcg cgccgaaccg gttctgcgtg
    38101 tcgtcgaacc cgcccggtcc tttcaccgtc gtgtagtgcc agccgccacc gcccaacaga
    38161 aacggagcca gccttgtggt acccagggga tagatcaacg ccgacaccat caccggatag
    38221 ctgtgcaccc gtgtgtcgcc gatatcattc ctgcgataat ccaccgaacc ttcaaatgcg
    38281 aagtaccggg acgggtggag ccgcacctgc ccgcctccga accagcggct cgacccgtcc
    38341 ttaggatcga cataggtggc gcgaccaccg atggagagca gcccgatatc gatttgctca
    38401 aacagccctt tatcgtccgc agcctgcgca cccgtggggg cggccatgct cccgaccgca
    38461 agcgtcaacc ccaatacgag catgagtatc gaaggggaaa ctgttttcat tgttcactcc
    38521 tctctgaatg tgtcgtgtag ggcatcatga ggacgtttgc tctcgttgag tgccgtgggc
    38581 gtcgaaccat tcatcgaccc agcgcttgac atcctcgcgc cggtcgccgt atcgctcctg
    38641 gatcttacct tccagcttat cgacgctccc ttcgatcgcc agcagatcgt cgtcggtaaa
    38701 gtcaccccac ttctgcttca attccccctt gaactgtttc cattttcctt gtaactggtc
    38761 ctggttgact gccaccgtga cagtgcccgg gtgttcgtca gccccatgtg tgctgccgaa
    38821 ggatcggtcg ccggcctggc tcagacccga tgtcaggacc atgcctgtca gagtcatcga
    38881 aagcagcctc atcctgagtt tcatggcctc tctcctccgc tacatcgcgt ggcgcgcgcc
    38941 ggcctgcgcg gccggcacgc ccgtctccat ctagtgatcg attttcgttt gaccactctg
    39001 cgcatcgaca tacaccttct gcgtcttgtt gttggcgtcg acgatctcca cttcgtagac
    39061 ggttcggccg tcttcctttc cgatctccgc ttcgacagcc ttacccggca ccgccttcaa
    39121 cgcgtgccgg acggcgtctt caagcgtgac cgtcgcagtg gaggccaact gcttgttggt
    39181 ttcgaacaat gcccaggcgg gactcgccag gactccggac atcaacgcca tcgcaataat
    39241 ttttttcatc tcgaatgctc cttgttatgc ttgttatgta tgattgatct gatattcaat
    39301 gaagacaggc ccaccgtcaa cgctggggtt gagcctggcg tccttcggtg cgcggcttgc
    39361 cctgttgcgc gtaccagtcg tcggcccatt tcaccacttc ttccttcttg tcgccgtagc
    39421 gttcctggac ccgcccgaca aatttgtcgt aatcgccttc gacctgcatc aggtcgtcgt
    39481 cagtgagctt gccccactgt tttttcactt cgcctttgaa ctgcacccac tttcctttga
    39541 attgatcggc attcatcgcg atcctccttg ttgtgatgtc atcgctgcgg aagcaggcga
    39601 cgctgatatc gccgcatctc cgcctcgact ttgctgggcg gcctctcgaa acacccggtg
    39661 gtccggagtg cggaaggcca aaccggtctt accgccacgg taaagcacga cggtgttcct
    39721 gccgtcatct tcaccggtcc tgacgacgac cgtctgcttg actcctttga atttcagatg
    39781 tttcatcggc gcctccatgc tgtacatgct tgttatatct ctcagggctt ccggccagga
    39841 ctggacatac ccctagtttt tacagcggct taccccggac gatcgcatcc gcatatacgt
    39901 acttcatcta ttccttcgca cgatgatgag gtcgagcaca ctcggctcgg ccgatcagcc
    39961 ggccggcaca aacatagaca tgacgttgtt cgtgaataag ggcagtcggc ccgggtgcgg
    40021 aaggatcacg cgccgtgtac agactcccgg caaaggatcg ccatggagcg ggcttcgatg
    40081 gtgtatggct cgccgccctt gaatacagcg ctcgatcgac gaggtgtagg atcgcaggta
    40141 tccaacatca ggacccagga ggtgcgctca tcgcatgccg gcaggacaaa tgggatagat
    40201 tcatgatggg cgttgaagag taagacaacc gtatcgtcga cgatggcgtt gcctcgtgca
    40261 tccttttctt cgatggcgtc gccggcgagg acgaggccga tggctctcaa gtgcccctga
    40321 ttccaatcgg cctcatccat ttctttgccg tccgagtgca accagacaat gtctgtgatg
    40381 tcggacccgt ggatccgccg cccttgaaag aaacgccggc gacgaaacac cggatggtcc
    40441 tgccgcaagg caatgagccg gcggacgaac gtcagcaagg tccggttctc ccggctgagg
    40501 tcccagttgt gccaactgat ttcattgtcc tgacagtagg cattattgtt cccgagctgc
    40561 gtccggccga tctcatcacc gccgcacaac atcggcaccc cctgcgagag gagcagagtg
    40621 gccaacatgt tgcgtttctg gcgacgacgg agttccagca cgtcccgatc ctcagtcggt
    40681 ccttccgccc cgcagttcca gctcaaattg tcgttatggc cgtcctggtt attctcgccg
    40741 ttggcctcat tatgtttttc gttgtaggac acaagatcct gcaagctgaa tccgtcatgc
    40801 gctgtgatga agttgatgct ggcaaacggg cgtcgcccgc tcatctcgta gagatcgctg
    40861 ctgccggaaa gccgatatgc cagctcggca acctgccctc cgtctccctt ccaataccgg
    40921 cggatcgcat cacggtactt tccgttccat tcagcccagc cgacagggaa attaccgacc
    40981 tggtatccgc ccgccccgag atcccagggt tcggcaatca gcttcacctg cgacagcacc
    41041 gggtcctggt gaatgatatc gaagaaggcg ctgaggcgat ccacctcatg cagttcacgg
    41101 gcaagcgtgg aggccagatc gaaccggaac ccgtctacat gcatgtccag cacccaatac
    41161 cgcaaactgt ccataatcaa ctgcagcgtt ctcggatgac gcacattcag gctgtttcca
    41221 cagccggtgt aatccatgta gtaacgcgga tcgtcgccca tcagcctgta ataggcggca
    41281 ttgtcgattc cgcgaaacga cagcgtcggc ccgagatggt tgccctcagc ggtgtggtta
    41341 tagaccacgt caagaatcac ctcgataccg gcgctgtgta acgtcttgac catcgtctta
    41401 aattcacgca catgccggcc tcgaaccgga gacagggcat accgcatgtc cggcgcgaag
    41461 aacccgatgg tgttataccc ccaatagttc gtgagtccct ggtcggtcaa atgtttgtcc
    41521 tgcaccgcat ggtgtaccgg gagaagctcg acggctgtca cacccagccc ctgcaaatat
    41581 tcgatgacgg gcggggtcga gagtccggcg taggtaccgc gcaaatgctc cggcacatcc
    41641 ggatgacgag cggtaaatcc tttgacatgc acttcgtaga tgaccgtctt cgaccagggt
    41701 gtacggagca gatggtcgcc tccccagctg aatgcttgat cgatcaccac acacttcgga
    41761 ctctcgtcgg cattgtcgcg atcgtccctc gccagatctc cctccgggtc gccgatgcga
    41821 tatccgaaca tgcgatcgga caatcggatc gtgtcggcga tggatttggc atagggatcg
    41881 atcagcagct tagaggggtt gaatcgatgc ccggcgccgg gctcataggg cccatccaca
    41941 cgataaccgt aatggaggcc ggggcgaccc tccggcaggt aggcgtgcca ggtttgattg
    42001 gtccgttcct ccagccgaat ccggtgggat tctttcgaag catccggccc atcgaacagg
    42061 cacaactcca ccgacgtggc attctccgaa aagagagcga aattgacccc ctccccgtcc
    42121 cacgtggctc ccagcggata gggtcggccc ggccatacct tcatcatgtc tccgtttcaa
    42181 tcacagggtg aaccgaggca accgtcgtgg gaaaccgggt catttcagga gactgaccag
    42241 atcttcggca tgctcttctt ctttcatcag aatttcttcc agcaacctgc gcgtggtcgg
    42301 atcatcgctg ccgacgaagg cgatcatgtc ccgataactc tcgatcgcga ttcgctccgc
    42361 caccagatcc tccttgatca tgtcttccag attctcacct tcgatgtatt cggaatggct
    42421 gcggtcggcc aagccctccg gtgaaaggtt cggttcacca cccagttgga cgatacgctc
    42481 ggccagctga tccgcatgcg cctgctcctc gcctgcgtgg gccagaaatt cagacttcac
    42541 gctttccgcg ttcatgccct ctgccatgaa atagtgccgt ctataccgca gcacgcagac
    42601 gatttcggtg gccagcgttt cattcaataa tttgatgacg gtctcccgat cggctttgta
    42661 gtcgaacgtc atcgctccct cttcgatatg ttgacgcgcg cgttctctga tcgtttgcac
    42721 atccttgatg atcgacacct ggggcttggc cggtttcata acgactcctt tcacgggcta
    42781 acaatgatgt tcgcgaacca tcgggttgtt atggggccgc aagatctaca cgcagatggg
    42841 gcaaagcgga ctctaacccc agctcggcgg ccggctcgac gcccggacca cggtcggtgg
    42901 ccggttgaag caggcccttc gactgggtac cgaccaccaa tgcgcaagcg gccagcgaga
    42961 gaaacagacc gacttgtttt ccggttgtat caaacggcat gacctgttct cctctctcat
    43021 ggatctcaat ttgcctgtgc gtagagtaga ggcagatggc cccggagacg tactaggagg
    43081 aaccctggac aggcaattca actgccctgg aaaccgaagc gtttttttta ttcacactag
    43141 gaggcgtgga cggaaagcac tccgaccagc tgttcgtggc gattcatcac cggtaaccag
    43201 tccgactgcg acccgtgcat gaccgaaatc gcgtccgcca gcaaatcatc ttccagacag
    43261 cagggcacag acgttctcat gaatccagaa atgggggcat cttgagaggc ttcctgcact
    43321 cggcggttca agccgatgtc tcgatcagtg agcattccga ccagccgtct gccgtcatac
    43381 accacgacga ccggcacgtt aagcgaggtc atcatcgaca tcgcctcctt cagacttgtc
    43441 ttaggagagg ccaccgtaac cgcggagctc atcgcgctct tcaccttccg agcctcgccc
    43501 gggagcaacc ccaactgctc atccagcgtg gattccaggt ataaactgga ccggtctcgt
    43561 ctcgccctgg cctcctccat tgttctagga ccttcgtgtt tcattccaga cctccctctt
    43621 tcactcacac acactaaacc gcctgcggac gaacttcatg caccgtgagt cgaagtctgc
    43681 catactcaac cggcttatcc ggatttctgg tgtatttcgt tttgtcttca ccgatcgaga
    43741 ctatgcttaa aggaagcgtg aggtgctgtt tgtaatgcgg gaatgcggtt tcgagcagga
    43801 gcgtgacggt ttggctttca cactcgatca cttcggcatt caagtcacgc tcatcctcaa
    43861 aatccaccgt gatccgctcc tcgggatgga tccaccgacg gatcgtcatg accttctcat
    43921 gggaatccat gcggtgcctc ctcgtggcgg tttgttcgag cgcatgctac cgttcagcac
    43981 agtgacaagt ggtatcatct cacgcacagt gcttcccgcc cactagcgat gtctctaggc
    44041 catctccgaa accacaggga cggggtcccg gcacatgaac tcctgttcca ggagctgtgg
    44101 aatgggcggc ctggtcggca gaggcggaat cggaggggga aagggttgcg gaggaaacgg
    44161 gcctgggtcc ggcccgggga aaggagccgg atcgggcggt tgcggatctg gatcgggtct
    44221 gatgacgagc agtttcatag tcacctcctc atcgcgggac gaccggggcc taacaggcgg
    44281 cctgcagtcg ctcctctacc gcgctccaag agatgttgct gaagaatgcc tcgatatact
    44341 gcttccgctc ctgcggttga taatccagca agtacgcatg ttcccacaca tccatgacga
    44401 ggatcggcag gaagccggca acatttccat gctcatgcag cgagacccaa tggttggaga
    44461 caaggccggt ggagggatcg atattgcaga tcgcccagcc cacacctcgc aacatgccca
    44521 cactgcaaaa gtccgtcttc caacgatcat aactcccgaa ggtccgctcg actgcctggc
    44581 cgaacggcga cccatccgcc ggacgatcca ttcctcctcg tctgagattg ccgaagtaat
    44641 attcgtgcag gatcattccg ttatattcaa accccaggcg cctcgtcagt tcggagtagg
    44701 ccggcatttc ttcttgatcc accgtcccgt cgctgagtat ctcatgtaag cgggctcgca
    44761 acgaattcgc cgccttgaca tacccttcgt acagcccgaa atgaaccgcg agactgtgat
    44821 ctgaaatgcc tgacaatccg aacagatcgt aggattttgc tgtatacggg tcgatcttcc
    44881 tcatgtgtcc ctccattaca tcacgtatgc atcacctgac gtcgtccgac gatgaaggac
    44941 accctatcac cgagccggtt cctcccccac taggtgatgc cctggcgtgg accgatgccg
    45001 gggagtttcc taggagcgcg accagtggta acgtggtatg agagaatgaa gttccgagga
    45061 atcgcaggag gtcgtcgaga cttcccggcg gctacaggtg taacgacgaa gagagcctgc
    45121 taaactgaga ccgttcaggg gtgtagtttc tgaaccgcct atcggaacga agcaggcacg
    45181 gaggcaggaa tactgtgtca aaagcacgtc tcgtcatcgc cgacgaccac agcatcgtgt
    45241 tggaggccta tcgccaactt ctcgagccgg aattcgaagt cgtcggcacg gccgaaaacg
    45301 gtcaagagct tctgaacatc gcgccggggc tggctcccga tatcatcatg ctcgatatct
    45361 ccatgccgac catgaacggc ctggatgtca cgaaacagct gcgtgccgca gtaccccggg
    45421 cgaaactgat ttttgtcacg atgatgagcg aaccgtttta catttcgcag gcattcgaac
    45481 tgggcgcctc cggctatgtg ctcaaacagt cggcttccac cgagctcctg tcggcgctga
    45541 atgccgccct caagaatcag cgctatatct ccccgcagct ctccctggag gtgcaggatg
    45601 ccattgaaac gccgtgggtg aagccggaag gattctcctc caaactgacg ccgcgccaac
    45661 aggaggtgtt gcaacttctc acgaaggggt tttccacgaa ggagattgcg gccacgatgc
    45721 aggtgtcgac caaggcggtg gaattccaca aaggcaatat cacgaggcgc ctgggcatcc
    45781 gcaccaccgc ggaattgacg agattcgccc tgtcacaggg cctcaccaag ctggacgacc
    45841 aacatccctg aacgctcaga cgtgaggccc cgggaccgtc ggttgctgcg gcatcgcgat
    45901 caagccgatc gtgatggcat gtttggtgag ttcggcggtg ctgtggacgc cgagttgttt
    45961 catgatgcgg gtcttgtgaa attcgacggt cttgatggac acgttcagag tcgaggcaat
    46021 ttccttggtg gattggcctt ccgcgatcaa ttgaagcact tcccgctgac gtgcactcaa
    46081 ctggtcattc gttgccgtgg cacgtgccgg gccgggctca gtaggattca gcgcctgctc
    46141 gaccagattt tccgccacac tcggggagac gtatagtcgc cctttcagca cttccttgat
    46201 cgcaaacacc aactcttccg cgacagactg cttcagcaca taaccggaca ctccggcgcg
    46261 aaacgcctcc gtgacaaaat actgctcgcc atgcatggtg agcaccagca acttgacttc
    46321 gggagtggac ttcttgatct ggcggcaggc atcgagcccg ttgagcaggg gaagcgcgat
    46381 gtccacgagc gcaatgtccg gcttctcctt ttccacggcc cgcaccaggg cccggccgtc
    46441 cgcaacccgg ccgcataact ggaagtcatg ctccaggagt ttttcgacac cttcagcaac
    46501 gagcgtatga tcatcggcta tgagaactct tggtttcatg ggtgtgctcc gggctgagat
    46561 gagaaggtgg ccagaatggc tgcaaaatcc taccaccctc ttgagacgat agcgaggggc
    46621 tttctccggt tagagctctt tcgaccagaa aaaaatgatt ccatggccgc ccttggtcgg
    46681 cggcgctcct cctggatcgc gataaatgcc gaaattatac gtgtaattca gctcgacttg
    46741 cgatttccaa ggggcaccgg tgaccagccc gcccccgagg gccggcatgg aatccgtaat
    46801 atttttgacc gcaccgttgc tcgtgaagac cggacgctcg accagtcccc atgtggcctc
    46861 aagataggga tagaggaaga acaaggcttc atatcgatac tgaagattgg cgacggcata
    46921 gcgggtcggg aacagctcgt tgaaggcgac gccgtggatc ataggaagcg agatggcttc
    46981 ccattcatat cccgtcggcc ttcccggcaa tcgaaaggcc gaaaaccgat ccaaatcctt
    47041 gccgatgcct ccatgcacac tggtgaggag ccgatgcttc tcgctctcaa tgaatggcag
    47101 cccggtcgcc gccaaggcat aggcgctggc ttgcagatag gtctgttctt tcctgaaatc
    47161 gggcgtatcg aagggcgcgc ctccccatcg atcccacttc gcccggtggc cgtgaatcag
    47221 atctcccccc atggtgaacc cttcgtgggg cagttccatc aagtttcgct ttaacgcatc
    47281 ggcgcggatt cgaaaatgga tacgtccctc atacgtgtct ttgggcacac catactgggc
    47341 agaggtatgg ccggttcctt tgaaccagcg atagccggct tcgtacgtga tggacatgtt
    47401 gacggcgctg tcctgccgaa agggatgcac gggcaaccga taccccaacc ctgcaccggc
    47461 aaaggcataa ctccactcca attccgtgcc acgttggcgc tgaccctgca catattccga
    47521 acggcccatg ggaagaatga agttatccca ggtgaagatc agcgaccagt tgggatactc
    47581 ccgcaggccg atcgtgtaat ccacgtcatt gaccactccg gagaacgtgc cgcggagccg
    47641 tcggttgtcg tcgtcccaat tacgccacac atagagggcg ccgaacggca gcatctccat
    47701 ttgactcggc ccgttcggaa tcgcctgaat cccgaaactt gccgccgtga cactcctccg
    47761 atcgcggggc ggcacataga tctcttcccc gaacagagtc gtccgaaact cttccccggc
    47821 aatgggagtc gggtcctctc cctgcgcaac attccctcca aggccacaga gaacgaggcc
    47881 caagccacat gcgagcactc gggccacctt acggactcct cttcgatgca taacggccat
    47941 atcgcgtctc tctgctcttt cacaacaccg cctcagccaa tcacttatgg tgtctcctta
    48001 taagacagtg gtgatatgac tcgtcctagt aaaacccctg gatgtgctgt ccagaacggc
    48061 gagtatttac ccggccattg cttaccgatc ctcaaagact gccggctaag agtgaatgta
    48121 tcgccggaag ggcatgtcat ttcacgcccg cagccggtgg ggctaagact aggtagatct
    48181 ccagtatcgc aatctgctga ggttcgatag gctcgttctg tgaccaggcg tcgtccgcaa
    48241 tctcaacagc atcagtcgac ctccacatcg tcaggccgaa ggtgtgcccg attcacactg
    48301 gccctcgccg gaatgtgcct cattgccacg aacgaactcc ttgccgcacc gattgaagtc
    48361 atctaccccg aaggtgtcag cgagggattc gtcacgctga aaagcatgga tggcaagaag
    48421 ctcgccgatg gggagttgag ccagttgacg acaggggccg accggttggc cagtcgtctc
    48481 acgtttcgct tcaccgacgg atccctctat gacgaaacgg tcaccttttc gcaaaagaag
    48541 catctggcca tgttgagcta tcaattgaac cagcgaggac cggcgttccc tgaacccctg
    48601 acgatttcgc tcaacggaga gacggggcag taccaggtgc gccggcacga gcaggcgaaa
    48661 accgaacaga cgatttccgg tcgcatagat ctgccggcgg atatctataa cggcatgacc
    48721 attacagcgt taaagaacct gcgcggcaga agcggtgcct ccatccacat ggtggtcttc
    48781 aacccggagc cgaaaattta tgaactcgat ttgaagctgg tcgagcatga agaaacgcgg
    48841 aacaccaacc ggaaggaagt gaaggccacg gccgcgcatt acctcttaac accgaagctg
    48901 ggatggttta tgagcgcgct tgcctcctta gtcagaagaa cgcttcctga atatcatttt
    48961 tggctgatta agaaggacgc gccggcgttt gtccgctttg aaggtagttt gtaccccgac
    49021 ggtccgatct ggcgaatcga gcaggtttca ccccgcgtga agtaaccgcg acccgcactt
    49081 tacggcaggc tgaggagggc ggcgtccaca ggaacgaggg aagcaggagt tcttccgatc
    49141 gcaaccacct cgatcgccct accgaattgg ttggtcgtcc cgtttccctt gatcacgact
    49201 gcatccccaa tctggaagga gcggcgaagc gcttcgctgg cgtctagcgg caatcggatc
    49261 tgcgtattat ccgacaagat catgccttga acggtgctct tgatcggatg atagagcagc
    49321 agacggatgg tccccgtcgc attcatttcc gtcacggaca aacgacgcgc ctgttcctgc
    49381 atcggaagat ccactcgtag cggaacgata aacgtcgttc ctcgtgtcag gttttcgatc
    49441 acatcgggct gtaccagtcg ttcattcgac tgacgccggc cgaacacctg tacgtgatcg
    49501 tccggtttga gtgcccgagt caagtgatcc gctgcgcgcg atgtcacata catataagtg
    49561 ccgtccacga gcaataatcc ttcgacttcg ccacgcggat cgatgaggta ccgctcaacc
    49621 accccatctg ccgcaaagga actggcggcc tcatcgtcag ccgcctgttc cgagtcggac
    49681 acctttccgg atgccacgcc cgggaaggcc tctatcggcg acgtcatgga gaaccaacag
    49741 accgcggtga aggccaaaag actttttcgg tgcataggag cagcctcgca ctcaagaaac
    49801 actcatagcg agtgattcag ccacgctgcc ggtgctcaac cgggctttcc gcccgtgaga
    49861 acccgtaggc cgaatcgacc aacgacacat gagaaaatgc ttgagggaag tttccgacca
    49921 atcgtttgtt cttaacatca tactcttcgg aaagcagtcc gacatcgttg gccaatccaa
    49981 ccagtcggtc atagagattg cgggcgtccc gccggcgccc cgccagaatg aggttgtcga
    50041 ccagccaaaa tgaacagggc aggaacaggc cctcctcgcc gagaagcccc tcgacctccc
    50101 ctgacgggtg ataccgcctg accagtccat ccaccgtcag ctcgcgttcg atcgcatcga
    50161 gggtgccctg cacgcgcgga tcggagaacg ggaggaagcc gaccaacggg atcatcaaca
    50221 gactcccatc caatgctgtc gagccataat attgcacgaa cgacccacga cccgaatcat
    50281 aaccttcgcg gcacacctgg tcgtgaatcg tggcacggag cgcgcgccat cgttccagcg
    50341 gcccctcgac gcgacaacgc tcaattgccc tgaccgctcg atccaccgcc acccaggcca
    50401 tgaccttgga gtgagtaaac tgccgacgcg gcccgcgcac ttcccaaatc ccttcatccg
    50461 gtcgatccca tgcggactcg agaaattcca ccagcgcatg ttgcacccgc caggcatcct
    50521 cgtccggcgc cagccctaag ttcctagcct gatgcagtgt atccatgatc tcgccataca
    50581 catcgagttg aaattgccgg gaagcggcat tcccgatgcg taccggcgcg gatcgctcgt
    50641 agcccggaaa ccagttcaac tccagttccg gcaaccgccg ctccccgctg atgccgtaca
    50701 ttatctgcaa gtcggatggt tttcccgcca cggcgcgcaa caaccattcg cgccaagcct
    50761 tggcttcgcc gcggtatcct gccatcagta acgcatacag cgtgaaggtg gcatctcgaa
    50821 tccagcaata ccggtaatcc caatttcttg ctccacccag cagttccggc agcgaggtgg
    50881 tcggagcggc cacaatggcg ccggtcggag cgtaggtgag cgccttcaac gtaatcaacg
    50941 aacggacgac tgcgtcccgc caagggccct ggtaggtgca tacggccgac catttccccc
    51001 accaagactc tgtctcagag caggctcgtt tcggcgacag cggcttcggg ggaacatcgt
    51061 gagaaggtcg ccacgtcagg gtaaaaggga tctcctctcc cgccgaaacc gtgaagtccg
    51121 cgaccgtcga aaaccccact ccccgcaatt cgacctcggt ctccaaatac accgcgtccg
    51181 gaccggccac agctttgaac aagcgcccgt cggtgctgac ccagggcacg atagatccgt
    51241 aatcgaaccg gagagtcatt tccattttca tccgcaccgt tccctcgact cctatcacga
    51301 tccgcaccac attgatgtcc tgatcgcgcg gcggcatgaa atcgacgacc ctcaccgtcc
    51361 ccgtccccgt cacataatga gtttccagca ccagggtatc cccccggtac cgtcgcctga
    51421 tggtcgtgat atcctggttt ggcgcaatcg accagcggcc atgttccgga gtgcccagca
    51481 gcgcggcgaa acaggccggg gagtcgaacc tcggaaggca gagccaatcc agcgatcctg
    51541 tacgtgacac aagggccgcc gtgtgagtgt ctccgatgag agcgtagtcc tcaatttttg
    51601 acggcatcat tgggagggct cggcaccagc atgaaaacgt tctggctcat ccattcaaac
    51661 tttacatccg gactcatacc gatacaaccc gtagccttct tcatcgactg tggcttgtct
    51721 gccactcagg aacattgata ttctcgcatc gtaatgacta cccattctca gagaaggaga
    51781 gcacatgcga aagatcgtcg ctaagaagac agaggctcgc aatgaaaagt ctgcgactcc
    51841 gaggcgcgcc aagcagcgcg cgattgagcc cgcaacccat acgatcaagc gaaaaccaag
    51901 caccggctct tccgcagcta ccgcacaaaa aaagcgagtc aagaaacagg acggaaaaat
    51961 gccgaacgtg gtcgcgaaaa gagcgtcctc ttccactcgc cgggcgaaaa ccatcggccg
    52021 ggccgtagga aagattctcg gcagggtgat cggcaccgtc gaacaaacag tggccaaggt
    52081 cataccgcgt ccgaagtcac cggcgccaag gaagacaggg acatcccgat gagacactaa
    52141 ccagggatag tcccagttcc tcggtggggg aatcaaccgt atgcttattc ttgcggttga
    52201 ttcaccaggg aggcacggga gatgaaaaca ataccggcac ggctttttgc agtgggagcc
    52261 ctgttgtatg caatgtccgc gggtctcgcc gcagcagcgc ccggttcctc accatcgtcc
    52321 gagcccgccc gtcccacgcc gcccccgagc ctggcgccac ctgctccccc gtcgcaaacc
    52381 gaccccggga tggtaaagca accagaacac ctgccacctc ctgcgcccga agccgtcgtg
    52441 cctccaccgg tcgtcgaccc taagatggcg atcgaccccg agaagcctcc acgtccgggt
    52501 cccccgccat caccgtcatc tggaaatcct ccagctagga ccaggtaggc tatcttccat
    52561 gacgaaagag agcatgctat gttgaaccct tcacttcagg actcgacgcc tcttacggaa
    52621 tcaccacgtc gccgaggtgt gcccatgagt gcacgcggaa ccttcgtgtc gatcctcgca
    52681 tggccgttga agggactcgt caggacgatc ctgagccctt ccctctatca gcaagtgcga
    52741 gtcacgttcc tcggtgaaca gtcaggattg ggactcgact ggcggtgggc aaattctctg
    52801 cgccgcccgc atcctatccg tcgggacttc ggttggcagg cgggacaacc gatcgatcgg
    52861 tattataccg agcagcactt tctaccggcc tgtcgcgcgg atattcgcgg cagggtgttg
    52921 gaaatcggag acgatcgata tacccggcaa ttcggtgcgg agaaggtcac ggtgtcggag
    52981 gtcctccatc ttcaccccga cactcccggc gccacgatct gcgcggatct gaccaaagcg
    53041 gatcacatcc cctccgacag tttcgattgc attattctca ccttcaccct gcaatttatc
    53101 ttcgatgtgc gggcggcgat caggactgtg gcacgcattc tcaaacccgg gggcgtcgcc
    53161 ctgctggtgt ttcccggtat cacccagatc tcccgctatg acatggacaa ttggggggag
    53221 ttctggcgct tcacctcctt gtcggcacga aggctgttcg aagaagtttt tcggcccgac
    53281 gatgtgacgg tcaccaccta tggcaatgtc ttagccgcga cagcaagctt gcaaggactg
    53341 atctcctcgg aattgcgtcg cgaggaattg gaccattggg accgggatta tgaattggtg
    53401 atcggcctgc gtgcggtgaa gcgataggac tcctggctca gggcagccca ttcgcctccc
    53461 cggctccctc ttcgcgcaat ccccgcggct tcccatagat caatcgtgca ccgggcttga
    53521 acgtgatgta gtaccagatc cattccacca tcacccgaaa ccggttccgg aagccgatca
    53581 aaaagaatat atggataaaa gaccaggcca gccaggcgac caggcccgaa gacgacacag
    53641 gtccgaatcg ggccacggcc ttggctctgc cgatggtggc taacatgcca cgatccctgt
    53701 agcgaaagaa caggcgatgc tccttccgga tgtcgtgggc aatcaacccg gctacatagc
    53761 gaccttcctg catcgcgacc ggcgccacgc ccggcagagc gcgcccttcg ggagactcgc
    53821 aatgcgcggc atcgccgatg acaaacgtcc acggatcacc cggcagactg agatctcgct
    53881 cgacgcggac gcgcccggtc tgatccaacg gcacatccaa cgatcgcaat atcggcgacg
    53941 cgatgttccc tgctgcccag agcacattgg cggttcgaat gaattgcgcg ccggccaaga
    54001 ccccctgccc ggttacgtca tggacccggc agttcaccat gacggtgacc ccgagcgcct
    54061 ccaatgccgt ctgcgccttc ccggacaaat caggcgggaa agcctcgagt atgcggcctc
    54121 caccctcgat taagatcacc cgaaactccg cccgatgcaa caccggaaaa tccggcgcca
    54181 tcgccctccg tccgatctcc ataagcgcgc cggccagctc gactccggtt ggcccgcctc
    54241 cgacaatgac gaaggtgaga tagtcgctcg cacgctgcgc gtcacccgcc tgttgggcct
    54301 gctcaaaggc catcagcatc cgctcgcgaa gctccaaggc atcgggaatg gttttcagcc
    54361 ccggggcatg catctcccaa tcttcatggc caaagtaggc atgtcgcgca ccaggcgcca
    54421 ggatcagtcg gtcataggga atcggcgcag tctcggagag caggacgcaa cgcgccgctc
    54481 gatcgatctt ggtgacctcc cccagcgtaa tccggatgtt ctgctgtcct cgaaacagcg
    54541 tccggagcgg ccaggcgata tcagccggtg acaacgcagc agtcgcgacc tggtagagca
    54601 gcggctgaaa gagatggtga ttaacgcgat ccacgagtac aacttgaacc gccgcccctt
    54661 cgagcgctct ggcagcagcc aagcccccga accctccccc gacgatcacc acacgctgca
    54721 tcgcgcgctc cctccttgcc aggctggcat atccgcatat cagctgcccg gaaccatgtg
    54781 tatcatgcca ctctatccca aaaaggaaag ggcaggctct ctcacaagag cctgccctat
    54841 atcacttcac acagggtgtc gaactagcgc ttgctgctcg gatggccgcc ttcgaatcca
    54901 ggggcctgtc cgggaggtaa tccactggtt ccactcgggc caccaccgcc cacgctttct
    54961 tcacccatcg gcccggttgc acccggcccc ttctgccctg gaatgccggg attaccggga
    55021 gcggagggcg cttcgcgaac ctgttccatc ttatccatat agtgcttgat atccggcgac
    55081 atgtccttgc tgctgttcat ggacaccgga ttttgaatgt cttctttctt gagattcaac
    55141 tgaagtttgt cgcccttcgt cacaaactga ctccagcgaa tgggaatcgg ccgcttcgat
    55201 tcgttgggca cgaacacgac gtattcgatg tcaccggtct tcgtgtcctt caagacttga
    55261 tgaatcttgc cgatctcggc gccctgtgta ttgtacacag aagcattggc gtatttgctc
    55321 cctttctcct cttcgagttt tccctgtcga accggtgtat ccgcatattc ttcaggcact
    55381 ccctgttttc caccggtcag atctgttttc ttgtcgggat tgatcgtttc tctcggggtg
    55441 gacgttcctg cacccttccc catggtggaa tcggatgtcc cgccctccct ggatccttgc
    55501 gtgaatcccg gtgaactcgt agcgagtaag agagcaatcg ttccggcgta aatggctatt
    55561 tgtcgcatca ttagattctc cttcttgcag gtgtgctacc gttggctgag aaactcagct
    55621 gaacgtctca ccagtagggc ttgtagccat aataggtgtg gaggcgctca ccccaggcgg
    55681 tatccgccat atccggccaa cgatccttat caaacccttc ggcctgctcc agccgttccc
    55741 tggccacgtt caaaatgaaa aaatctcccc ctgcggacat cgtcaaggcc tcccagggga
    55801 gcgcgaacag tttgtcgccc aacccaagaa agccgccgaa cgagagcacc gcataagcaa
    55861 tgcgtccctt ttcgagatcg atcatcagtt cattgatctc tcccagatct tctcccgcct
    55921 gattctgtac gggcgtgccc tctatcgtag aagccgccag caatcgtcgc cctgtatgta
    55981 acgccatgag aaaccctcct tgtgttgata tgcgatgctc tgaggcagac aaatcgacct
    56041 cgtactctta gtcataacgc ccgaggtcga gaggaacagc taggaaggcc cctagaaatt
    56101 ctgtcggcgg gagaagaatc agtctgagcg aatcagcagg gccacaggac aatgtttcag
    56161 gacttgcccg acaggcagta cctggcgctc gccccggttg accgtctcga accgttctcc
    56221 ggtgaacagg tgctcatagg ctgatccggc tttccaggac ggaaccgtca accacgtgtc
    56281 ccgccaggtg tcatcaggtc tgccctcgtc tctcgtcgcg gccgatccgg ccatgaaacg
    56341 cggagcgacg gctaccaccg cttgatcctg gtgtaatcgc gcgaatgcgc agaggtggaa
    56401 ctggtgaggg ccctcgcagt cgagtggaac gtagtcacca tccagataca gatccggata
    56461 cgctcgcctg tggcgcagtg ccgcctgcag gataaatagt ttcagtcgcc cgtcttgcca
    56521 tgattccgcg agacaacgaa taaactcgag acgatcgccc gcctcgtttg ttgcggctcc
    56581 atcgaactcc ttcaacatcc tggcgcgaag cgcgtactcc accggacggc ggttgtccgg
    56641 atcgacaaga ttcaattccc ataattctgc gccctggtaa cagtccggaa cgccgggtgc
    56701 ggccaccttc accaacaact gcgacaagcc gttgtacatg ccatatcgag cgaccaccgc
    56761 ctgaaacggc agaaagtcgt tcagaaacgg attgggttcc cgcgatgaca agatggcgcg
    56821 aatgaaccgg cacagcgcag cttcgtaatc cgggtgggga ttgatccaac tggtgtgcgt
    56881 cttcgcttct cgaacagcct tgatcatgta ccgctcaatg cgcgtcacaa actcttcgta
    56941 ccggacctca tccatcgtca tcagcggcca ggcccccacc agtgtctgat agaaaagata
    57001 ttcgtcatta cgatcgggag caggcctctc ctcgacttcg gtccggtacc gcctattgag
    57061 cctggcccag cgggcgatgg ccttgctcca acgctctcga agttccggga gcacattgat
    57121 tcgagcgcgc acatcttcgc cacgtttcgt gtcatgggtt gaagttgccg acatcgagta
    57181 gggccagccg gcgcggcgtt cccgaatggc ctggtgaaac gcctccacgg aggagccgaa
    57241 atgagccgga tctcccccca cctcattgag cgagatcagt cggttgtaga catagaaggc
    57301 cgtgtcctcg acgcccttgg ccatcaccgg acctgtcgtc tgctgaaact tcgtgacgaa
    57361 ccggatctga tcctccttcg tcaatcctgt ggacgggtcc agctttccca agagcaggtc
    57421 gcggacgaag tcgaatatct gaccgttcag tgttggattg cgccgtttgg ccctggcgac
    57481 agccagcacg atataggcct gatcgcggtc aagcggagcc tcgcggtcgg tcgtcagata
    57541 cgagcgatag acgggaaaac aggcaatgat ttcccggacg gcatgggtca ggctgttgag
    57601 ggtaaagtcc cggtagtgcc ggtcccgctc ggacagtcga ttcaattggt ggccgagcac
    57661 attgagttcg ctggacatgg aggcccgcat gatcagtttc ttggactggt acactacgtc
    57721 acgatacggt cgccgttctc cgataaagcg cgtatacagt gcctccatcg cctgttcttg
    57781 gtccgcccgg acaaacaatc cattcaccag attgagaaag tcatagccgg tggtaccggc
    57841 gacgggccag ctgcggggaa gctgttcgcc ttcacccagg atcttctcga ccacgacgaa
    57901 cagcgagggc gtgtcttctt cgcgctcccg aggcaattct gcagcagccc aggcttgcag
    57961 ctgctgcaga taatgctccg gatcgtacaa cccatcgaca tggtcgatcc gcaggccggt
    58021 cgctatccct cgcttaagca aacggaagat cagccggtga gactcagtga agacagattc
    58081 ctcttcggtc ctgatggcgg ccaattcatt gacgtcgaaa aatcgccgat aattgatctc
    58141 ctccgacgcc acacgccagg acgcgagcct gtaactttgt tcgttcagca gagcgtcgag
    58201 cttgtcgaag cttgccgaca gacctttcgt gccgttatag gtctccacgt tggccagcac
    58261 atgcgcgtgc acctcccggt aatccgtgat caacgcagcc aaccttctcc tgacaattcc
    58321 tgtttcccga taacgctcgg cgaccttttc cggatcacgt tcccggcgtg gcggcaaatg
    58381 ggacagcgcg gtcaggatac tctgcagttc gaggatggct gcatgcccgg atcccaggag
    58441 ctcgatgagc tcggtgatcc ggaaggacaa gatcgccgcc caggacttgg gagcgagtgg
    58501 aagccgctgc cggtaatacg ataccaggaa cccgccctcc tcgtactgca ggcagatctc
    58561 ctgattttcg agcaccgttc catactgttc cccgaggatg gggagcagca ccttatcttc
    58621 gagttctcgt ttcagcggtg tccaatcgat gtcgaacgcc gcggcgtatc gagagcccgg
    58681 gccgttttcg agcacatccc accaccaacg gttgtccggg gtgccgatgc ccatgtgatt
    58741 gggaacgaca tcgaccaata atcccatgtc gtgctgcttc agcgcttcgg cgaagtgggc
    58801 gaagtcttct tcgcttccca actcaggatt caagacacca gggtccacca gatcatagcc
    58861 gtgcatgctt tccggtgccg ccttgagcag ggaggaggca taacaatcgg taatacccaa
    58921 gccatgcagg tagggcacca gggcggcggc gtccttgaag gtgaaggtat ggttgaattg
    58981 gagacggtag gtcgagacag gaatcctcgg catgatctgt tgaacctcgg gcggctctca
    59041 gtgggacgac aatcggcgct tgaacagctc ggcccgtacg tccggcttgg gaaacaccat
    59101 gacatcggaa gccatccagg aagcgttcgg tccgacctca cgatccccat gccccagtac
    59161 gacccccgca ggaatttcat tgttccggtc gatcacgact cgacgcaaat gcgccttcgc
    59221 accgatcctg gcgtggtcga gaatgaccga atcttctatg tgggcaccgg catcgatccg
    59281 cacatgtcga ccgatgatgc agcgacgaat gtccgcttcg atcaagcggc tcccctctcc
    59341 gatcactgca tggtccagat aacaattcac tacactggcg ggtgggccga aggacggttc
    59401 actccggatg ggccactccg gattgcgcaa atcgttaagc ggcgtgtccc ccagaagatc
    59461 catgttggct tgccaatagg cgggaatggt cccgacatcc cgccaatatc caggctcctc
    59521 gtagtacccg agtccgggaa cgtcattctc ctgaaaattg tacgccatca ctcgttcctg
    59581 gcggatgagg cgtgggagaa tatctttccc gaaatcatgc gttccctccc gctccgcgtt
    59641 gtcctccaac gctctgatca acacctccgt attgaatagg tagttgccca tggacgagag
    59701 ggcgcgatcc ggttgtcccg gcaacccttt aggctccgag ggtttctcct caaatccgac
    59761 gatccggccg ctccgatcga cttcgacgat accgaatccc tgcgccgacg ccatggggac
    59821 tggaagggtt gcgaccgaga cgtccgcctc ccgctcctga tgagcacgta ccatctggcg
    59881 aacgtccata cggtaaatgt ggtcggctcc gaacaccgcc acgatgtcgg gagtgaagtc
    59941 gtggatcaga ttgagatttt ggtagacggc atccgccgtg ccttcatacc agccacgatg
    60001 cagattcatc tgcggaggga ccaccgtcac aaactgccgg tcaccatcgc cgatacgcca
    60061 ggctcgccgg agatgctcga ttagcgattg agagcggtat tgcaccatga catgcatggc
    60121 ggtgataccg gagttgtaga aattgctcag cacaaaatct atgagacggt aggtgcctcc
    60181 gaagggaact gccggtttac tgcgtgcccg cgttaaaggc atcagtcgtt cccccttgcc
    60241 gccggccaga atcaagccca ggacacgagg ttggttcaga cgcgcatgag ttcccatatt
    60301 attcctcggc aaatccgaat gagtgcggca gaaatgcctc gtttcattcg cttcaaccct
    60361 acagaaagcg accaaccagg aatactggac acgtccctag taacgactgc ctggcggagg
    60421 ctttcacaat gatagtttcc ccactcgctc ttatcagcat gttgcgcacg ggacatccgg
    60481 ctaaagcgcg cgcataaagg ccgccagcgc agacttctcg tcccgattga gttggagacc
    60541 agccacaagg ttaaaaaatt cgaccgtgtc ctccaacgtc aacaggcgcc catcgtgaag
    60601 atatggcggc gaatccttga tcccccgtag cgcgaatgat ttgatcgggc cgtcggcctc
    60661 ggcaccgaat cgttccagaa acagatcatg gagcatgtga tcggtatagg ccggaggctg
    60721 atggcaggtg acacagcggg ccttcccgaa gaacaggtcc tcgcctgcga tctcctgctg
    60781 actcgctcgc gcacgatcca aacgccctgt cataggatcc agctttggcg ccggtgggaa
    60841 atcgatcatg ttttggattt gcgccatatg tgccacctgc aggcggtcga tgacattcat
    60901 gcctttcttc atcgcgcgta tcggatcgcc gttaaaatag gctgtccgct gttcaaattc
    60961 ggtgaagtcc tcgatcgatc tcagacttcg ctttgaacca tgaacctgct ggttgtacat
    61021 acctctgaga ctcaccgtgt ccagacgtag ccggtcgcgt tgagggcggg tgtccggatt
    61081 cagatgaaac tgtcccgttg tgtggaaatt gacatggcaa tccaagcagg ccactcccag
    61141 actgggcttg ggggatttcc gatccggcgt caggttgaat tcttcttgcg ggaactgagt
    61201 cactagcatc ctcaaaccat ccaactgaac gggcgtgatg agtccgcgaa aaaggcggtc
    61261 aaaattatcc gcgctcagca cctctccctg cgatacgtcg cccaattcgg gatgggtggt
    61321 caggaaaatg ggcgggggaa attcagggag aaaacaatcc ggaaggtcga agtctatgtc
    61381 gaaacgctca agccggggga attgctcaat ttgcttttgg ggaaacacct ggccgccgac
    61441 ctcatgtttg acgtgcggta aacgcatgaa tccggccggg aacactttct gccggcgaat
    61501 gtcctcggcg cttatgtgag agagcttgtc ccaggtcata ccttccttga gctgtaccgt
    61561 gggtccgatc ggctgcggct tcccctttgt catgacggcc ccggcctgcg tcttacattc
    61621 aagcgtataa cgcgcctgca ataacgcagc gtgattggcc atcaccttgg gtttgtcggc
    61681 catgtctttc ctcatgactt cttccgcaat gtgtgcagtc gattgaccgg atggagcacc
    61741 gaacagatcg taagaaaagt ccacaccgtc gaaccccttt tccttcgctt tttcgatcac
    61801 ggattgtggc tggttccgct tctcctcgcc atgcccttga gccccgacaa atacgagccc
    61861 ggacacgagg gcgatgccga aaacagacgc acccagaagc ccgaccgtgg cagctcgcct
    61921 tcttcgtgac gtacgcatgt gagacctcat atggttgaga gaaacgttcg cggacggtta
    61981 gtgagtttga atttaccggc gcatggcctt ttgcactact aggtagttcc ctggcgatga
    62041 ggacctctat cgtcaataac cagggttgcc cctagtgctc tctctgagcg gcattcctta
    62101 cgatcacaag tgtaattgat gccacgaacg ggaggacgca ccatgcactg tcatcgttgc
    62161 aataatttga tgttcccgat tgccttacag gactggggcg gcggattaat gaggaaagac
    62221 tcgcccgcgt ggcgttgctt cgcctgcgga gagattatgg atcagcagat ccgggacaac
    62281 cggagcaagg accatgagag cgaggaggcc cggcataaca gaggagcacg acatcgtatc
    62341 aatgccatag cggtacagcg atagcaggac cggccaggaa cggcgtctca gtcgtcctgg
    62401 tcggactcct catcggagac cgatgccacg gatgcgcgcc ctgcattcac gatcagacag
    62461 gctgtttccc agatgtcacc tgcaatgaga tggggccaac gcatgctggt catatcccag
    62521 tcagtctcac tcccgttcaa acagagtata gtgttgcacc ccaacaagcg tccgacctca
    62581 atgttgtcca gccggtcgct gacgagccat gagcaggcca gatcaatatg caatgccgcc
    62641 gtcgcatcaa ggaagggcat gatctgtctg ctgagcgggg ccggcctggt gctatggcgg
    62701 acatcatcgc atgtcctgtt tccctcatca ccttcggcgt agacgggaag ggcgtccgat
    62761 gtgaatatga cgatggtgaa ccccgccagg gtcaagagtc gataggctct tcgttctgtt
    62821 tcgccggccg tagctgaagc ggcccgagaa tacattccac cactcccggc catttccctc
    62881 gctacgacga acaccgcctt atctcgcatc ccgatcgaca tgtggtttct cacgtctatg
    62941 ttgtgctcct cgacttccgg cgaggcctta tggacctcga gttcgaagcg cgttgaggaa
    63001 cgttgattcc ggactggcgc gccctcgaga gcccaatggc cacagcttgc ttacgagatt
    63061 tcaccggatg ttggccggtt ttcatatgct caatctcctc tttcacgaaa gcaccggctt
    63121 gcgtcgtcgc agcctttccc tctcgtttcg ccttgcgcgc ctcttcgaga gtcttttttt
    63181 tcggcatgct gaatctcctc ggttatggcg tggcctgcgt tgggtcgtag ccctcgattg
    63241 gatgccgccc gatcgtcagc acgaccgcca cgagccctag aagtaggatc aggtaaaaac
    63301 caatcctgaa ggcgtctgtg tagggggtgt tgaagattcc gaacgcatac agcgcagtgc
    63361 tgatcattcc gatcagcaca atcgcgacga gcaacgtgac catgacgtca cctcactttc
    63421 atcggtgggt tttcatcccg cggatccctt tgtggaggct catcggaggg tgggtcttgc
    63481 cgcggaggag gatcatccgg aggttcctgc cttggcgact ttttatgacc cggctctctg
    63541 actggtgttg cagcgcccat gggtagatct ccttgacctg ttcacgttca ccgctcagcc
    63601 tgtctccaca atgacgtcca gcctccgccg aagcgatctc tcagaggcgc tcgaaccagg
    63661 gccagcgata ttgccgcaat cccgacgacg aggctgccgc aagcccgttc gtcgggatac
    63721 tccagaagaa acggtgcggc gacgagccag acacccagca caagattggc ccattgcacc
    63781 gcccgcaggc actccgacat cgcgatcgcc ccgaaggcgg ccatccacac cccgacgaac
    63841 tggttgctga ggcgggcatg gccgccatac cccatcacat ccggggccgc cgccaaccag
    63901 actccgagca tgccggtcac tgccgggggc cacatgatcg ggccgccttt ctttggatga
    63961 ttatccgctc cataccgcct tgccctcgcc tttccaaaag gtcgtccaca ccgctttcct
    64021 ctgctcttta gcccgtttca ggacctgcaa actggccaac acttcgtcca tcgccggtcc
    64081 gatcatcaca acggaaatca cggccgacaa gagacacaat gtgcaccacg cgtgcaagag
    64141 aagcggttgg gctgtcacca gcaggacact gaccaacccc aacggcccta ccgccaaccc
    64201 gaacaggacc accatccagg gcatggtttt ccaccgtgtg gtcccaccga tagcaccggc
    64261 cacggcatcg atcccatacg ccatagcccc gagaatcgca tccggtaccg gaagcactcg
    64321 tgagagcggc gaatgcaaca cggtctcgct gccccgcccg aaccacggtt cccaaacaga
    64381 gtcgaggatt ccccattgat agagcccgag ataccctgaa atgagaaagc cgagaatcgc
    64441 cacgccgatc agccacagac gttcggacca gctcgacggg ttataggtcc atcctggcgg
    64501 tcgaagagcg tcggttcccg tcactgtgtt tcctccagct cagcggggag gctcaactga
    64561 tgagcccgat accaggccac cgggttccgc ttaagcgcat cgatcatctt cggcagcgtg
    64621 tcccgaagtg aatgccgggg ctcccaatcg agccactcct tggcgcgact cacgtccaag
    64681 gcgtaatgat cgtcggcaaa atcgatcatc cagggtttaa tgaacggatc ctccaacagc
    64741 ggcgtcaccc cctgaagcca ggcgcctgtc ttggccacca tctttggaat tcgtacggtg
    64801 tcccattcct cgccatgcag tagccggccg aatcggtgct gtaattcgtc gtatgtcagc
    64861 ggctccggct cacccagcaa catcacggcc tggtcaggca gactccggcg acgttcgacc
    64921 acgcgcacaa tggcgtccac caggtcttcg agatgcagaa aggattggcc gtgggagata
    64981 tgaccgggaa acatcttgct cgtaatgtga cgttcattga tgcgctcgat ttgatgtgcc
    65041 agtggaatcg agtcacaatc atcgtcatac accccggcga ttcgcaagac ggccgttgga
    65101 tagttccccc gttccttctc aagcatccgc tccgtctcca ctttcgattg cggatagggc
    65161 caacggggat cgagcggcca ttcctcagtg atcgcttctc ctggttcgca gggtttatgc
    65221 acgagcatcg tgctggagaa gatgaatcgc gccacccgga acccgagggt atgcagggcg
    65281 cgaagcaggc gtcgagtccc ctcgaccgtg acttcactgt acttggaact gggctcaccc
    65341 gagaaatcga aatacgcggc cagatgaaca accgcggcga tatccgagcc aaagcgccct
    65401 ttaaccatgg tgagggcctg gacgacgctg tcgtccgagg tgagatcaca ggggatattc
    65461 tccacagagg agggcggatg cggttcgccg ggccggtcca gtcctgccac tcgaaattca
    65521 gcagacagcc ggtccgcggt tcgagcgccg atgagcccgc tgcttcctgt caccaacacc
    65581 aactccttcg tcgtggttgc ttgcatgcga cctgtgctca gcaagagcaa tgccgaagcg
    65641 cattcagata gccccatcca gacattacag ctctatcatt gccatgaacg ggcgagttcg
    65701 ccccttacag ccaccctttt aaagccgggg gaatcttctt attcgcgagg actgagacct
    65761 gaatcatgtc tcgcgaaggc tctcagttcg gatggattgt cccatgagag acattttgtc
    65821 cccgagatcc ggggatcagc ctcatcatga caaccgggcc atggtcgtcc gagctgccct
    65881 ctggtggaac agaacagacc tattatggtt ggagtgaaaa ggcgcggctc ttgctaagag
    65941 agtaggcgtg accacactca acatgaggga gtcatcatga aatgtattac tcgtagcgaa
    66001 tatccataca tcggcattcg aatgatgatc tgcttcggcc tcatcggaat gtggggcgcc
    66061 ttgctaccga tggcggaatc agcggaagcc ggacagcgta tcagtccggg cgaaggagct
    66121 caattgcgag acgatgcgga aaaactcctg aaagggaatc gctctgtctt aggaaaggtg
    66181 ttggcaatca cgtcggatca gatcaaagtc gacatcgggg aagtgcagcc ccgcttcctc
    66241 cccctcaaac aggcccagca gaaagggttc cctggtgtcg ccgaaggaga tgatttgatc
    66301 gtcgtgctga atgcgcaaaa tctcttggtg gactatcacc cgctggacgg gcaagccagc
    66361 gcccatacga ttattcgagg agagatcgcg caaaaccttc cggtgggcca ggaacaggtc
    66421 gtgattaagt ccgcagggaa agaaatgtcc ttcactatac gttcacaggc acgcagcaaa
    66481 gttgccgcga tacccgtcgg cgtggccgct gtgttcttga tcgatgagac gaatcagatt
    66541 gcggatgtag gactgtccgg tcaacagacc agcaaacagg ccgatcgact cacagactcc
    66601 atgccgccca tcaagggcgc ccataagcag atagacggaa ccgttaccag cccgctccgg
    66661 gccaatcaca ttaccgttcg aacggccaat gggtctgaga aaccttttga agtacgggag
    66721 ctccttcaca aaaaactcgc cgctcttcgt aaaggggatg ccgtcattct gctcgtagat
    66781 acagataata aagtgatcga tgttgcggtg ccaccgccgg gacggtgagc acaaatgctc
    66841 ccgcgcattc atagactgcc atgcgcggga gcggcagtcg ccatgccgta ttgacggagc
    66901 ttccgaagca gcgtcttccg atggatgccg agaatgacgg ccgatcgacc cacatcatgc
    66961 cggtgtgaat cgagcacccg caagatgtgg tctcgttcaa gattctccag cgtcacccat
    67021 ccctgagcgc gcgcgaggga ctccggcgca gcgttcttga cgaccggagg aagatcctcc
    67081 ggatagatca cggcatgcgg actcaaggcg acggctctct caatcgcatg ttcgagttcc
    67141 cgaacgttgc ccggccacca gtatcgactc aacaattcca tggcttcgga ggagatctcc
    67201 ttcaccggcg gaatttttat cgccccgtac aggttgagga accgttgcgc cagggctgga
    67261 atgtcctccg ggcgctctct caagggcggt atgtcgatcg tgacgacatt cattcgataa
    67321 tagagatctt cgcggaatgt ccctgcgcgg acgagaggtt cgaggggcag cttggaagcc
    67381 gcaataatcc gcacgtccag cgacgtcgaa gcgctgctcc cgacacgccg gatctccttc
    67441 tcttggatga ctcgtagtag cttggcttgc agagccggag acaagtcagc gacctcgtcc
    67501 agaaaacagg tcccggaatg tgccttttca aggagacctt tgcgcgatga caccgccccg
    67561 gtaaaagatc cacgttcatg tccgaacaat tcggattcga gcagactgtc tgcaagtgcc
    67621 cccccatcga cggtgacgaa ggggccgctg cttcgagggc cgctgacgtg aatggcgcga
    67681 gcgatgagtt cctttccggt accgctctcg ccttgtaata aaacggtgct gtcggtttgt
    67741 gccacccggg cgatcgcttt gtacacatgc accatgcgtg gactactgcc gacgagacta
    67801 ccgaacggcg aagcgtcttc cggctgcagg tctgaggcgg tgggactctc tgaaggcgga
    67861 gaggtatgtt ctagtgcccg tctggcgacg catcgcactt catcgacaat aaatggttta
    67921 gtcaaataat cgaaggcacc ggccttcagc ccctctacgg cagtcctgag agaaccgttg
    67981 gccgtcatca acaccacggg catattcggg aagcgggtgc gcatttcagt gagaaacgtc
    68041 aggccatcga gctgaggcag ttgcaaatcc gttaaaatca gatccgctga tccctgctcg
    68101 aaatatcgcc gggcgctttt cgggtcacgc gcggatgaca cctcataacc ctcatgggac
    68161 aaaatgtcgc gcaattcctg agccgcgtca gcgttgctgt cgacgagaag aattcgggca
    68221 tgtgaggtag acatctgtaa acgttcctga aggcaatcaa tcaggcccag tatagaaatt
    68281 gtagagtacg ccccaggggc gacatcgtaa cctggaagaa accctggccc acgaggcaat
    68341 cgagaaccat gagtcggcct cagcgtggta cagcatggaa tggttggaga catgacgtga
    68401 gtagtcctcc tcctctgcct ggcgttggtc atcattgccg catcatcaca tgcttcctgg
    68461 cgctcctgag cgcgctcatt cctcctgtca ccagcgctca gattcttcaa aaaccagagc
    68521 ctattccacc tgcgcccatt ccagggccga ttccaaaacc gattcctccg tcaccgatcc
    68581 cggctcctgc tccaaagccg atcccccctg cccctcccca ttaaccccgg ttgctcctga
    68641 tgttcagttg tgaagaaaat agtggtctca tacggaggac aagttgtccc gcgcgggata
    68701 cttcgctccc ctcacgacca cgccgttgcc cccactcgca ctccatcgcg cgcattttag
    68761 agagtgcgcc ctccggcatg ccgtttgctc atctgtccga ttgagtcagg tcgaaacact
    68821 cagagcactc cggtgcaacg ctctgggcga gagtcacctc acggcaggtt tggtctcgcc
    68881 ctccacagaa tggggcaggt atgaaacacc tgagcctgta tgcgctgatg gtagtggtcg
    68941 cggcaggaat ctcgcttcca tgcgaggcgt tggaatttac cgcagagcga ctatcccgta
    69001 ccggtcagga agttcaccat gcccggatct tctaccgcga ccacatgtgg cggttggaat
    69061 acaacgagcc tggggccgtc agcgcgacaa tcgtcagggc cgaccgtaac caggtctggc
    69121 acctgatccc ctcgatccat cacttcagga cggagtcata cggcagcgac tatgcgctgc
    69181 acctcacgat ccgactggat aacgagacat cacgagaatt catcggcacg cagaccttag
    69241 atgggcatcc gaccacgctg tacgaagtgg ttgcgaccgg tcccggagaa caaaaggaaa
    69301 cctattatca atgggtcgct accgatatga attttccact caagctcgtc aaaaaggacg
    69361 gtgactggat ggtggaatat cgtgacgtca agctcggacg gctcgccgat tcgtttttcc
    69421 agcttccgca ccggtatgtg ccgatggagc cataagggga tccttcatga ccaattcaac
    69481 gtccctgcat caggcccccg gagcaggcgg tcatgtcatc gtcggcctcc tgccgagcgt
    69541 ggctgccgcc agactggctc ttgaatctct ccgtttgcag ggtgtttcag acgagcagat
    69601 cggactggcc atgcggcaag cggaacaacc cctctcaacg accgaggagg atcggccctt
    69661 cccccacagc ggaagaggcc acccaggggg ttgtaggagg cggtatcatt ggaaggcttg
    69721 ccgggttgct gacagcggca ggcttcgcaa ccatccccgg tctggctccc ctgctggcag
    69781 gtggatcgct catttcttat attgggagca accggtgcct ccatcgtggc ggcaagtgga
    69841 ctcggcgctg tcactagtgg cctcgtcggc gcgttgatca gtatcaatct acctgaaacc
    69901 gcgctccacc attaggagca agcggtgcat cggggcaatg tgctcatcac cgttacaacc
    69961 gacggggagc ctcagaagat tcagaatcta ctgacttcca caggggccga caccgacgtg
    70021 caatcgcaac gcccacgatg atcgccgatt acatgggttc gtcgacaaac ctgatatggg
    70081 atgagcgcta gggagtggaa ggaaccgccg taaaacttcc cggacacatt cagaatctga
    70141 tcgcgatccg gctttgcccc gagagggggt cgagggtggt gaccatttcc cgtactttcc
    70201 gatctaccac cggttcgata cgttccctga gaatgtcctc acaggataca aacatggctt
    70261 catgcacttg gcgaagcaag gcgcacccct cctcggatcg agccaatctc atttcagcgg
    70321 gaacggatcc ctgtctcgta agggtcacgt caatttcatc cggggacatc tgaacccgga
    70381 catgcgcata gtttgacttc atgaactcgg tgtgaaagtt caagatcgcc aacatcacgg
    70441 catattcgat gtccgcctta ttccgagcct gcgccatatt gacctctcac ccatggtgat
    70501 tgcagcaatt ccggcaccgt ctctcaactt cacagcctgt ccagcggctc cttacgacgt
    70561 cgcagtcacg actgcaagcc gggcccgttc tccgcgaggc tccacacggg cctgtcgatg
    70621 tttcaaaatg actggatcga caatatcacc gcacatgagg caatgcatgg cggaaatccc
    70681 tgcccctgcg tttcggatat atgatcgtag atctcggcat acatcgggct gttacatcgc
    70741 aggcaattca taaccgtctc catggtgtat ctgctggtgc tgttggtcaa agccggaatg
    70801 tctcctcgat caccgtgcaa acctcagtcc acgcaggtgt ggcggcagga cagcgtttcg
    70861 catgcccgga tttctcgtaa aaatgagcga ttctacccag gccgcgaccg gttatgacgg
    70921 ggatcaacgg ccgcgccggc tcgattgcct tccccatggt gtgtgcgcgc gcacggatgc
    70981 acgaacaagc gtgcacgggt acccgtcgtc tagtatcggc gaacacacgt gcggcgatcc
    71041 aacttcggcc gtgacgatgt gtagagcggt atgtgggagc ggctgaaccg gaccggattc
    71101 aggagcagtc ggggtctgga tcggtcaggc ggaggaatcg tgcatgaacg ggggaacgtc
    71161 acgtcagacc ctgggtttgc gacggcgatc caacatccac cgcgtcaccc ccaattgctt
    71221 cgcggccagg gtcttgttgc cgccggatcg ggccagcacc tcttcgatga tccgatcctc
    71281 cagatcggcc aacgattgag ctccgacccg aaagctgatg tggacgtccc cggaatcgga
    71341 tccgtccgcc tcaacagagt aggcgtccag ggacgccgcg accggtatcg atagggcccc
    71401 ctgaacatcg atgggaaaat tcgacgcctc cagcgtcgtc ccggcacaaa acaggacagc
    71461 ccgctcgatg agattgctga gttcgcgaac attgccggga aggtgataac cccggagcag
    71521 agcttgcgcg ctttcaccaa gggtacgcac gggcttcttg agcgccagcg cggagcgatt
    71581 caggaattgc tccgccaagg gaatgatatc ttcccgccgc tcgcggagcg ggggcaccgt
    71641 cagagtcacc acattcaaac gaaaatataa atcttcacgg aactcgccct tcgccacagc
    71701 ctctttcaga ttgcgattgg tcgccgccat cacccgcaca tcgatgggaa tattcaccgc
    71761 gcttccgacc cgccggatgg tgcgctcctc cagcacacgc agcaatttgg cttgcagtgg
    71821 cagcggcaga tcggcaatct cgtcgaagaa caccgtcccg ccttcggcca tctcgatcaa
    71881 gccgcacttt cgaccggtcg ccccggtaaa ggatcccttc tcgtgcccga acagctcgct
    71941 ctcgaacagg tcgcgcggaa tcgacggaca gtccacctct acacatggcc tgcctgcgcg
    72001 ggggctattg tagtggatca cacgccccat atattgctta cctgtgcccg tttcgccctg
    72061 cagcagcacc gtcacccgat cgttcttgat gagctcctgc acctgggcga ggaaacttct
    72121 ggtgacagga ctcttggcga tcacccgatc cagcgcataa cgggcggcat cctgcccgct
    72181 gtgcaattgc acctggcgct gcagcgtcag gaactccgtc gctcgacgca gcgtatgaag
    72241 cagatcgtcc atgtcgatgc tcttggcgac gaaatcgaac gccccgagcc gcatggcctc
    72301 caccgcgtcc ttcaccgtgc ctctcgccgt caacacaatc acgggcagcg ccggcgacag
    72361 ctgtttcacg cgggccaaca cctcgagacc cgagaaattc ggcatgacga ggtcgaccac
    72421 cagcacatcc ggttcgaacg accgcaggag ccctaaggct tcatctccgg actccgccgt
    72481 ctgcaccaca tacccctggt cgcgcagccg caacgcgaaa gcttcacgaa ccgaggcctc
    72541 atcttccagc aataagagat gccacgccat gttatgcctc gtgcttcagg ggaagccaca
    72601 catccacgac cgctccgctc cccggggggc tctgaatgat gagctgtccg tgatgccgct
    72661 ccacgatgtc gcgcgtgatg gtcaacccaa gccccacccc cttcgccttc ccgttcgtaa
    72721 agaagggctc gtagatatgt gtgagatcct caccggcaat ccccttgcca gaatccgcga
    72781 atcgcatcaa cacacctgga acacgccgca cgtccggcga gagggtcaca ttgagattcc
    72841 cgccacctgg catcgcatcg atcgcattca tcgcgatatt caggcagacc tgcaggatat
    72901 gggctcgtga tgcgtcaacc gcgtgcagat ccggagccag cgacgacttg accagaatgt
    72961 ggtgtttgat caggttcggt cgaaggagcg tcaacgtatc gtcgagcact gcgggaagct
    73021 cacacaccgc gatatcaaag gggcgagggc gcaattgtcc caaatgagat tccagaagtt
    73081 catcgatccg cgccgcctcc ctggcgatca accggcaccg gtcacgcgcc tcctgcggca
    73141 gcgaggcctg atcggcaaga tgcgtggcca ggctgcccag gccgatgagc ggattgcgaa
    73201 cctcgtggag catgccgccg gccagttggc cgaccagctt gacctgttcc gtatgtcgca
    73261 acgcctccaa catctgttcg cgctcactca ggtccgtgag gatcgccatg aagtattgcg
    73321 tggtcccatc cgacgccttc accggactca cgctctccca caccagaaac tcagacccgt
    73381 ctttccgccg attgacgaat cgttcgacaa agggatgacc ggcgcgaatc gacgcccaca
    73441 gacgctcata aaactcgggc ggatgttttc cggacttgag aatggcggga cgcctgccca
    73501 aggcctcctc acgggtccaa ccggtcatcc gctcaagggc cgggttccac tccatgatcc
    73561 ggccggtggt gtccgtcatc atcacgccgt cctgggcgga caggaagagg cgatggtaga
    73621 aatccatctg cgtttcttca tgacgccgcc gctccagaac cgtgccgatg gtattcgcca
    73681 tcgtgcagag aaattcctgc tccttctggt tgaacttgcg aacccgcttg gaatgggcgg
    73741 tcatcacccc gtacacccgg tcctcgacca gcatcggcac gcacatcccc gcaaccgcgc
    73801 catgttccgt gagcaatttg gacgcggaaa acctggtttc tcggcgaaag tcgtcgacca
    73861 caaccggaat ccgttcccgg atcgcatatc cggcctgaga atgggtgccg ccttcgatcg
    73921 tgagtttgcc gatgagatcg ctgcgtaacc cgatccccgc catgatgtag agatggccgt
    73981 cccgctcacg cggcaccagg atcttgcaga gttccacttc caacgccgtg gccgtctcct
    74041 gcaccgcttc attcatcagc gcctgagccg acaggccgct gaccgccaga gtaccgatac
    74101 gcgccaacgc cgattgaaag cgggcgacgt gcgaggcgtc ctgtgcgagc agcacccgct
    74161 cggtcacatc ttccaccacc atcagcacac cgcgctcacc acggtagggc accacattga
    74221 accggcaggc ataatagagc gggcgcccgt cctgcgcctc cactgtatat tcgaccgtct
    74281 ttcgggctcc gagcgcacgt gtgttccagg caatggcgtc atccagcagg cgcttccgaa
    74341 gggcatgctc tcctaccccc acccggggag acgcactctc tactaattcc gcgaattgag
    74401 aattttcgaa gataagaagg ccttggcaga cgaaacagag acccccgtga atacagtcgc
    74461 tcaaccaggc caggacctca gcggcggcga tctccgatcg gaccggcctg gatgccttgg
    74521 aacggctacg atgggtagag gccatgagtg tcgcgctcgc cgggaatgac acgtgcctgg
    74581 ttcagtacac gcatgcgtgc gggctcagta taccgagcag gtctccaccc gtcgagaggg
    74641 agaaaccggt cctgacgccg gctacgggac cacaaaatga tcccgacggg tgaccgccac
    74701 gtcactgacg aacatggcgc cggaatgttt gtgaaagagc gggcccagtc ccgccagaat
    74761 cggctcgatc cgttcctcgg gcacgacggt gaaaatgatc acctggctgc tggtgtcgtc
    74821 gaacagcagg tggccttcat ggaacccgtg atgtcccttg ccggagacgt tattgataat
    74881 cgtatagccg gtggcgccga ctcgatcgag caggttcgtc actaatttga ggtgctcacc
    74941 ctcgaccacg atgcggattt ctttcatcgg atgcaacgtc agcatacttt cgcccccctc
    75001 ctcctgttct tccaggcccc tcgatgccaa agactattta cgctcattcc accctgcctt
    75061 gcgttagggg cgcgaggcag ccggctcctc gccctcccgc accggcccgc cggatcccgg
    75121 ctgcgctcct gactcacctg catcgggacg ccgaccggaa ctcccctcca attcctggcg
    75181 cagttccaat tcttccgaca ccaatcgcca gccctgcata atgtcgtaat ggtaaaatgc
    75241 cgcttcgttc ggctcacaca ccaccagcga cacccactca ttctggaaca gccgttcgag
    75301 tccgggatgt ttcgcaatca ccgactcgac gagggcgcgc ggcgcctcaa tcaccgtcaa
    75361 caagcgcatc ggctcatggt aggggaccgg accgtccagg acggtctgag ccggcaggcc
    75421 gaggcgaagg tcgctggtcg ctccgctcat cacacccact cgtccgacga tattgtggta
    75481 caccttgctc ccgctgccgt acacctcgtt gtcgacggtg gagaaataat gctccatgtt
    75541 gatccattgg gccacgatga ggggagcggt catgatcgtc tccaggagtt tgcccgagga
    75601 atcctggcga tagtcgtagg aatgcagaaa tgaccgcccc tggagatcca gcggccgcgt
    75661 gagttcacga cgcccgatga tgaggaggtt gttcttcgac aggccccact cagggcggac
    75721 ctgggcccaa tcaacactgc gccggctcgc ccgttgctgc gggtcgcggc gggtcggttt
    75781 cgccccaggt ctctccaacg ccagaccacg ttccaacgcc gcctgcgttc ccgcctcctt
    75841 cagatcctcc aacaaccgca cgaggtcctt ccggtgcgtc ggcggcacat cctccaaatc
    75901 tacgatgcgg acatcgttcc tggtcgtatc gtgcagcgcc gcgacaaaat gcgtatcgcc
    75961 ggggatcttg accccccgcg actccagcac ctttcgaacg gccggattgt tggccatcga
    76021 cgcgaaggcc cgcgcgttcg ggaggccgct gttgccgccg caggcgccgc aatccagggc
    76081 ggattcgtac ggattgttgt ccgacgtact cccgtgggaa cacatcacca ccagccgaga
    76141 aaaaccggac gtaaatccca tgagacggag cgccgcttcg acgccgtagg cctgttccgc
    76201 gacagaaaat ccatgcaggg taatgcgctg catgcgggtc gacatgcccc gcggtgtgag
    76261 ggccaactcc cggcgcaacc gttcatgcag cgcatccgct tcggagggag caattccgag
    76321 cgcgttcgca acctctcccc tgctttgctc gtcctgattc tccagcgccc cgaggcggat
    76381 cgtctcgatc aaggccggcg tgatcgcgga accgagcgcg ggaaactctc gccggagcgc
    76441 ggcacggatg gacgcgcgct gttccgccgc caccatttct tccgcctctt ctctggtcaa
    76501 cttgtcgatc gtcagagtcg tcgccagcgg cggcagccac agtcccttca cccaggaggt
    76561 cagacggtga taccagagtg gacagagggt cttgccgaag aagggaacgc tgaagaacca
    76621 tcccagggct tcgaccatga cgtagggggt aatgacgttt tccttgagat cgtggaggag
    76681 ctcatgaccg gccttgttga gtcgcgcaaa cagccggtgc cggctcgccg ccgccccatg
    76741 gtaactccgc gggatctccc tgatgtgatt cttgggtttc aacagcacgg gcgaatgcgt
    76801 gaccggatgt tctccgccga acgcctggta cttgacggga atgccgaaaa atcccgccac
    76861 ccccaatgtc tcgtatcctc cgagctgttc gagatgccgc cgcaacacct cggaccgaat
    76921 atcgatacaa aacaccatct gcgccagggg acgtgacgat ccacccgacg agacccgatc
    76981 gccggcgtcc cgcagctgcg ccgcgacgct gcgcaattgc ccgacgatgt cccggcggtg
    77041 acgtgcttca aacgcgtcca gccaacgctg gctctgctgc ccggagggga atccatccaa
    77101 ccagttcagt atcgtcgaca catcggtagg ctgcgtttcg ctgatctgtt ccggatcgac
    77161 acccatcgca gccgacaggg cccacaaccg ccggacatct cgcttcacgg cttcagaagc
    77221 ccgccacccg gcggtctgtt catagacctg tctcccgatg ccattccact catccggctg
    77281 ggcggtccga tgaagacgcc gccactcccg cgcctctgcg gcggctgagg cgggcaaccg
    77341 tccctccgtc aattgacgcc ggagccagtg ggcataggga gcctgttcga tggacgcacg
    77401 gatcgcatcg taatgcccgg cgatgtccag cgactgtcga cagtgcagcg ccacgaattc
    77461 gcgttcgtaa aacaaccgaa ccgccaggta tttcaccaga tcgaccggat agcgggcctg
    77521 ccagggatga tgctgctcct ccgaccgcca cttgatatag ccggtccatc caggcatggc
    77581 ggacagatgc caggcgaaat aggactccca tgccgacttg gggatgccca tcacggccag
    77641 actatccagt acggcctctt ccggccggtc acccaaggca tcgatcttct gggacgcgtc
    77701 caccacaccc aataaccgca ggctggcgtc ataacgggcc aaccgcttcc acgcgcgata
    77761 aaaggtatgc tgccggtccg gcatcgtcca agaggcttcc ccttcatcca gaaatgcgct
    77821 gcaccatttg atcatctgct cgttgatatc cgctacgagc gctgtcccca acgtgcgatc
    77881 gcaccaggct gagagggttt cgtgggacaa caaatcgacc gtctcccagg gaatcagcgt
    77941 atccgcctgg ctggttctcg tctgccccac cgcagtcgca agccacaagg ccagctgatc
    78001 cagcctgtcc tcaaccggca cggaaggatc cgcatacgcc tcagccgcct cactgagccc
    78061 gtgcaccatg gagagccgca acaggtccag atgggaaatt tcacgccccg cgaacagaac
    78121 ccgccgctcg ctggccaagg gggcaagcac ccgggtgagg tcctcctgac gaatgcggcc
    78181 ctgttcgaaa tagcgacgat acgaggcgct gggcagatag ccttttcccc cgagcagctg
    78241 ttccccgcgt ttcaccgcct gagcgaacgg aagatcttcc agcccgtgca gagggttgtg
    78301 gtgaataaac gtgcgcatgg gccagtagga cgaaatcgcc tccccggcca actccacatg
    78361 ggagcgcaac tccatccgct cggcatctgt gaaggtgcgt gattcaaccg ccatgccacc
    78421 actccgtttc agtctgaccg cgctcagccg gactaccggc gaaaggcgac ccgaccgatc
    78481 atgcgaacca gatcctcaac atagaggccg ttgagaaaca tcacgtaagc ccggacgcgc
    78541 aaggtctcgg tccaggccgg catcagcagg ctgacgccct tggctttccc atagatcatt
    78601 ccccaggcgc cgacaatcaa gatcgcagcg agcccgatta ccaattcgaa gaacgagcga
    78661 ttccattctg ccgctcgaag ataggactcc gctatcccgg ggcccggaaa caggagatgc
    78721 gtaaatgcct cccccgccca caggtaggtg agcccgatga acgccagggt gccgagcatg
    78781 gtcagcgaca cggcccagga tgccccggtg tgcaatcgat acagagtaaa catcgcctgg
    78841 gaagtcgtca cccacccgaa gaacaggaaa atgaccgccc cctgcgcatc ctgcaacgga
    78901 atactgacca cgccatgcac gaccagcagg atgaccagcg gcagcaccaa cgtcgcgact
    78961 agcccggtgg tccaggtcat cggtgagaag gtatgcgcct catccgcccg gccgacggga
    79021 ggaagtttaa attccgtccg cgccttatga atgacggaac cggaattgag gaacagcgtc
    79081 gccttgaaca ggccatgcgc gcagaggtgg aacacggcga gggcaaaggc acccaagccg
    79141 cactccatga ccatataccc catctgaccc atggtggaat agacgagccg gcgcttcacg
    79201 ctggtctgcg tgagcatcgc cgtggcgccg atcagggccg taatgccgcc gatgacaaac
    79261 atcaaatgga gcgtcgtcgg cgcgagaccg aacagaggcg ccagccggtt cacgagaaac
    79321 cctccggcat tgacgatccc cgcgtgcatc agggccgaaa ccggggtcgg cgcttcgatc
    79381 gtaccgatca accagaaatg aaacggaaat tgggcggact tggtcatgac cgagaggacc
    79441 aacaataggg tcacgacgag aggcacatcg cagtcccatt gcagacccgg ccagaccgtc
    79501 aacggcgccg gggctgtctg caaccgggca aagaggtcgg tcatatccca agtcccgtac
    79561 gatccgtaca ggaccagcag cgccacagca aatgcggcat cgccgaagct ctggacccag
    79621 aaggtctttt gccccgcatc cctggccgca cgactatcgg ggttgcacac cagcaacctc
    79681 gccaa
//
LOCUS       DAORRQ010000012        77529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_12_length_77529_cov_58.739745, whole genome shotgun sequence.
ACCESSION   DAORRQ010000012 DAORRQ010000000
VERSION     DAORRQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 77529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77529
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12_length_77529_cov_58.739745"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK224_05580"
     CDS             complement(<1..387)
                     /locus_tag="PK224_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15238.1"
                     /translation="MSRHGRSYRRLGHVNICALGLSVAMMLAPSFVTAQTSPPASTPQ
                     NQPGQNPQSLGTLPLQNPLAQQSSITATASGASAPQSNTIPSSQGKSFGTIGKGLPGM
                     TGGPALTAPMGSQDPSSRYMRPTVIPP"
     gene            complement(407..712)
                     /locus_tag="PK224_05585"
     CDS             complement(407..712)
                     /locus_tag="PK224_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15239.1"
                     /translation="MNMLMLAFRSSLKERVHVLLEQCDVRAYTEMPETTGAGQTGPAD
                     GVSFYPGVNNVIMVAVDDAHAERVERAVHAWRDEVLQRPGGQKPSITVFSWPCRQLV"
     gene            complement(785..1891)
                     /locus_tag="PK224_05590"
     CDS             complement(785..1891)
                     /locus_tag="PK224_05590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRB15240.1"
                     /translation="MAQAKTKKNGAVGKKLVGMADVVIAAAQRAKDPTFEIPIRALSN
                     VSFNPRRGLIEMGEKKQSRSFFNVGMAKKFMQTMLVADALAELQQADLTTSLREIYYR
                     TKHTIQDSHENTFDTQDESDPIIEDLEVSLVALREELHVRAENSGSIVGPVVFGDDGD
                     RVDCAKLGKGGYSVPSIVEPEYLEIRRCTADFLLLVEKGTQWNRLSEDKFWRRYNCVL
                     LTGNGQPPRGVRRLARRLHEEHKLPVYVLVDNDPWGYYIYSVVKQGSINLAFESQRMA
                     IPKAKFIGLSSADPERYELPRNVGIKLNEKDITRAKELLNYQWFQKPAWQQEIKRMLA
                     NGLKYELDALANKDFQYLTKKYLPRKLKEKDWLD"
     gene            complement(1900..4014)
                     /locus_tag="PK224_05595"
     CDS             complement(1900..4014)
                     /locus_tag="PK224_05595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HRB15241.1"
                     /translation="MATKTSSPRAQSSAPAPQSETTVSESVASRKKSTSREESSAAVT
                     AVEMGARQREISVSEFFTKNRHLLGFDNPRKALLTCVKEAVDNSLDACEEAGILPEVT
                     VRLETVANGQAPVAPSQATRFRITVTDNGPGIVRQQIPRVFAKLLYGSKFHRMRMSRG
                     QQGIGISAAGMYGQLTTGKPVKVISRTGPRAAAHYFEVQIDTKKNEPLVHENKQIDWA
                     QPQGTEVALEVEGRYQKGRASVDEWLEQTSIANPHVRLVYHTPEGDTKEYPRTYHELP
                     PSPKEIKPHPYGIEFGALLKMLQDTKSHTVAGFLASDFCRVSPALAEEICKAAKVSPA
                     TKPRDVKHQAAETLYKTIQTTKIMAPPTNCISPIGEKAILSGLYKQIKGEFYTAVSRP
                     PAVYRGYPFIIEAGLAFGKGPDEAAKPQQAAVPLAEGEDQGNDTEQARVIRYANRVPL
                     LYQQSACATFKAVLSTTWRNYGVSQSRGALPAGPMVIFVHMASVWVPFTSESKEAIAD
                     YDEIQKEITLALREAGRRLGIFLRRRERAASEFRRRNIFDLYIEEVVAACNRLKGGNL
                     PTEKLKEQLHKIASSRTGGLKTDEALGKTGAGPEGLPHSIIVTAEGIEGETEMAQQTP
                     GSPAPATKTAEAEPITETKNPVKAKRGSKETLKVGKGKSVGATKAKVGQQALFADEQP
                     STKQSAPLKKKPAQVSATRKRK"
     gene            complement(4446..4919)
                     /locus_tag="PK224_05600"
     CDS             complement(4446..4919)
                     /locus_tag="PK224_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15242.1"
                     /translation="MNFRLVLTVVALGFIVLLLVIGIRTAVRWLKIHYPQRANAVLFG
                     VCLLLAGAGVLIAIEMKDQPTFRPNDLLTLQEPVVARTIVSDRESRSITCVVDLHEHL
                     GVLELDNERGSLRALVESNNTSVSLYCPIGAEVRIEAAWLHHPTITHRQADTDGP"
     gene            complement(4916..6499)
                     /locus_tag="PK224_05605"
     CDS             complement(4916..6499)
                     /locus_tag="PK224_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15243.1"
                     /translation="MVSLTAMSKTTPSVTELPPPKILIVDDDPDIALVLHDLLEHVGY
                     RVSVAGTGAEALSKAKAESLSAVVLDLMLPDMDGLSVLTLLKQIDPVLPVIMLTAFVE
                     VAKKHSSLTEGAFGYLTKPYDAEELKALIRRAVGVKHLSIEAAATKQALTASEDRFRE
                     VVQTAPDAIVLADGEGKILSWNSAAERLFGYLAGEVLGESLTMIMPERYRARHVQALA
                     RVRTTGDLRLKGTVVTMHGLHKDGREFPIEMSLSSWISDGQRVHCGIVRDITARKEAE
                     ARLLRQQIEQQALLDLIPAMVWYKDAHNRILRANRRAAASIKKTVAEIEGRSTDEFYP
                     EEAERYHQDDLEVIVSGRPKLGIIEPYQTGDGEKRWVQTDKVPYLDPQGNVLGVLVFA
                     QDITERKRTEEALRESADCLRLVMESASNGIIVVDAEGMILLTNPHVDTQFGYERGEL
                     PGQPVERLMPARLRPQASDRAGVFLIRPDDRSVSPVRECVGLRKDGTEFPFRMTLTPL
                     MTTNGLYSVVAVNQAQGIA"
     gene            complement(6674..6850)
                     /locus_tag="PK224_05610"
     CDS             complement(6674..6850)
                     /locus_tag="PK224_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15244.1"
                     /translation="MKNVEMTVEGTVLTIKVDLSKEFGPSASGKTTIIASTEGNVTIP
                     GREEKIGLNVYRKK"
     gene            complement(6860..7063)
                     /locus_tag="PK224_05615"
     CDS             complement(6860..7063)
                     /locus_tag="PK224_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRB15245.1"
                     /translation="MSVARVTEIIAASPKSFDDAIHLGIARANKTLQNVKSAWIEDQK
                     VDIEDGKITQYRVALKVTFVLKD"
     gene            7291..8001
                     /locus_tag="PK224_05620"
     CDS             7291..8001
                     /locus_tag="PK224_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HRB15246.1"
                     /translation="MPTEIEIQITPLFSTPLLVFTPAHHERINAELSQLILAREASVP
                     SHRDTEVIGWSSPHDLSMMDWAGSPLRELFAPVIEVAKQVTTLSDRCRHAARRPDWEV
                     VEVWANVQRNGGTNAAHSHPGSFWAGVYYVDVGEVCPTLGKGGELQIYDPRGCLPRML
                     APYLQYSMTELHDAGTSISYSPAAGQCLLFPGWLFHAVNTYRGTAPRISVAFNLDPVL
                     QQGPLSSAHADAVGGSRR"
     gene            complement(8068..8658)
                     /locus_tag="PK224_05625"
     CDS             complement(8068..8658)
                     /locus_tag="PK224_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15247.1"
                     /translation="MARPAMYRCRRSNNCMVVVGLALLALTAGCSPYEWRHENQWESR
                     DQIWLAEASQVKVRAAQSRVFDTADRLKTIQGVVATCQDLGFAVEVLDETLGIVSGKK
                     FDDYESLPWGDPSYHLYRDDGLLILTRQFRSWGPFYHRSNLVRLTVTVRPRGNRQVIV
                     RASAQYYLKAIESPEPYQQFFRALEQALFIQANALP"
     gene            complement(8662..9099)
                     /locus_tag="PK224_05630"
     CDS             complement(8662..9099)
                     /locus_tag="PK224_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15248.1"
                     /translation="MNWTQLVGAVVVMMGSVILQGCVAPEPRQELFAPTDAQMKIRSA
                     QTRTFEVKEQTVAMRGVIAALQDLGFIIERANEPLGLVTAARFAEPNYYDVLGVTVTV
                     RPQQDGKTMIRANAIYNSKPIEDPKVYQNFFAAVERSLFLVKD"
     gene            complement(9112..9687)
                     /locus_tag="PK224_05635"
     CDS             complement(9112..9687)
                     /locus_tag="PK224_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15249.1"
                     /translation="MQRLPAAVLLLMGLLVLPQGCVQQTQPAELFQLTPESSANRAMQ
                     TRFFDTENDQELLSASAAALQDLGFQVEESVREVGFLRAAKERSAREYGQYRNRFFIW
                     LLSLGHVVIPIDLHQKIAASLVTRPLNEAHSRQEVRIVFYRAVWKGDGQADRNYIPPG
                     EQKMEMIRDPEIYQQFFAKLSKAVFLEPHSI"
     gene            complement(9704..10105)
                     /gene="bamE"
                     /locus_tag="PK224_05640"
     CDS             complement(9704..10105)
                     /gene="bamE"
                     /locus_tag="PK224_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRB15250.1"
                     /translation="MRHMRWGVAVLGLCVLTGVTGCGGRQAEIKEDRLTVGRVQGEVK
                     VGMSAAQVAELLGSPNIVTTDDKRREVWIYDKVSTDRVDTASSSYAGLIILGANSSDR
                     SSSQRQRTLTIIIKYDEDKKVRDFAYNSTQF"
     gene            10281..10793
                     /locus_tag="PK224_05645"
     CDS             10281..10793
                     /locus_tag="PK224_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRB15251.1"
                     /translation="MPMPDAISNDDVEAAWRTLVEQTRTGVLLTLRNGHPFGSHVPYL
                     LGDDWARVYLHLSRLALHTQHLLEDARVSLFLAEPDKPGKNPLALQRINLQGTASILP
                     PTDMAYASVKQRYLAQFPQSQMMFGFGDFALWELRMEDAHLVLGFGQAYQARSTTPGQ
                     WQHQKPDKKS"
     gene            10958..11398
                     /locus_tag="PK224_05650"
     CDS             10958..11398
                     /locus_tag="PK224_05650"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRB15252.1"
                     /translation="MFFIVAVIAGYMLLLVGLAVASPGIGFTGSLLHLMPDDWRDAAH
                     VPAYGLLAWLAMIGFRLRGWPIRYALLVGIAFAGVFGLWTEVAQGTAPGREASLHDLS
                     NDLLGAMMAGALMLWQHSTSTRTDQFEPALRANLPHLRKGTSSR"
     gene            11395..11736
                     /locus_tag="PK224_05655"
     CDS             11395..11736
                     /locus_tag="PK224_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB15253.1"
                     /translation="MNTAYRARGARRIPVQYPIYYSNGTFQTIGVMEDFNASGGRIRG
                     KESVSVGMELVVIVIQPTPETPMLIRRATVRWSKGHDFGIALSGVPPQSESELKAMAK
                     VMLPGLWSCLN"
     gene            complement(11824..11946)
                     /locus_tag="PK224_05660"
     CDS             complement(11824..11946)
                     /locus_tag="PK224_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15254.1"
                     /translation="MSWENWAVAIGLGALLMMFFVLMELFFAGPRQPLDHVRRK"
     gene            complement(12000..12944)
                     /locus_tag="PK224_05665"
     CDS             complement(12000..12944)
                     /locus_tag="PK224_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15255.1"
                     /translation="MGRKYDLVDVVVAVGLVATLFGGYLLVTAADGFWQAPVAPAANM
                     TIVTHDPSTGMQYLQPVLGQAILHDVLLDREAGAALSASAMELNRAVSESQLISTTLL
                     SPLVLAELRAFGQEADHRARMQYVMGKSVVNQTRRGLASGVLSADQYAGEFNTNLIQR
                     AESIGQRMHDQFESTRQATLGRSIVEAIQEEDGMAAAVQERIGRAVIQVTQAQEGYAE
                     AKAAGQIQLASAMSAAVRTDALVDRLALLKEMPAGRTIVAYHSTATADISRGALVLAC
                     LGLIALFVGGLAFSSKREVDSIPLWKLETLLQLHRSGR"
     gene            complement(13496..13630)
                     /locus_tag="PK224_05670"
     CDS             complement(13496..13630)
                     /locus_tag="PK224_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15256.1"
                     /translation="MGDTFVSMRVGVDGVLLTQRSQGAGGPVPLLKDRWLGASDGNRA
                     "
     gene            13945..15579
                     /gene="lnt"
                     /locus_tag="PK224_05675"
     CDS             13945..15579
                     /gene="lnt"
                     /locus_tag="PK224_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRB15257.1"
                     /translation="MTISRARLILLACASGLLYPLSFPAFDLGIVAWFALVPLHIAVE
                     YLSPGRAFRIGWLSGTLAFTGSMFWVITAMNVYGKVPFPIATLVMLLLTVYLGLYMAV
                     YAGGLSWIRTAFPTLGFLPAPFLWVTLELIRTYFLSGLPWALFGYSQYQWLPIIQLAD
                     HFGVYGVSFLLVLVNAALAETIIWGFKAYRGFQIRSFPWPSSVAATAGFGVALFYGTT
                     LLSSAQHVPTRTLSVGVVQPNVEQAQKWDVAFRQETMARYDRLTAGLGDNLDLIVWPE
                     AATPFVFEMEPQYRAVVNDMARRAGAPLLFGSPALRRHPDGRPFLLNSAYLLATDGQI
                     LGRYDKQHLVPFGEYIPFHNSFLFFLDKLVEGIGDFEAGPGSTLLMFKPREKNPPAVT
                     AATLTDFHVKFGVVICYEVIFPNLVRQFAANGADFMVTVTNDAWFGPSSAPYQHFGMV
                     VFRAVENHVAFARAANTGISGFIDPYGRVVQQSPIFTQEALRGTIAVSHQQTFYSQYG
                     DLFAYACAILIALLCLTGFFSHDTEPAGGEPAGRPA"
     gene            15598..16741
                     /gene="prfB"
                     /locus_tag="PK224_05680"
     CDS             join(15598..15666,15668..16741)
                     /gene="prfB"
                     /locus_tag="PK224_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRB15258.1"
                     /translation="MLDDVRTRVRTLGEQLAQLRGHLDLARMTAELNDIEVKTSQPDF
                     WKDTQAAAKVNRRKAALDRELSRWRETERRQNDIEALLELALESGDTGLEQELLAELG
                     PFEKAVAQFRIELLLSGELDSSNAIFAIHPGAGGTESQDWAQMLLRMYVRWAEQKGFK
                     VETLDLLAGDEAGIKSATLSVSGPYAYGYLKAEAGVHRLVRISPFDANKRRHTSFASV
                     FVYPELDDDVQVVIDDKDLRVDTFRAGGAGGQNVNKVETAIRITHIPTGIVVQCQNER
                     SQLQNRNGAMKILKARLFEVEQRKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTGYEVGQVSAVMDGELDGFIEAYLQRKLTGGKAPEPVAMKDDDL"
     gene            16843..18324
                     /gene="lysS"
                     /locus_tag="PK224_05685"
     CDS             16843..18324
                     /gene="lysS"
                     /locus_tag="PK224_05685"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRB15259.1"
                     /translation="MDELNDQRQQRIKKLDQLRQLGVAPYGTRFEVKDRAGQLTRLHG
                     EKTKDVLEQEQISCTIAGRIVGLRRFGKAAFAVLQDGADRLQVYLKKDTLGEQAHQIS
                     EGLDLGDWIGVTGVLFRTKTNEFTVEVRTLTFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPQVHQIFALRSRIVSGIRAFLIERGFLEVETPMMHPIPGGAAAKPFVTHHNA
                     LGAELYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYADY
                     HDLIVLTEELFGRLAQDILGTTTIDYQGTQINLGSPWRRWSYHQSILEVNSLPTSVLT
                     NRDEALAAAQRLGVEVSPKESLVNILNEIFEETVEPRLQQPTFITDYPIEISPLARRK
                     DSDPSLTDRFELYIAGREIANAFSELNDPLDQRERFEAQAAKHAAGDEEAHRVDEDFL
                     RALEYGMPPTAGEGIGIDRLVMLFTNQSSIRDVVLFPQLRPEK"
     gene            18327..19604
                     /locus_tag="PK224_05690"
     CDS             18327..19604
                     /locus_tag="PK224_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRB15260.1"
                     /translation="MALPYEIFIGLRYLRAKRRNRTISFNTIVSIAGITLGVAALIGT
                     VGIMTGFKEDVQSKILGTTAHILVNDRMKESMVDYEDQVKKVAAVPDVVAATPFIFRQ
                     VLLTSPSGVQGIILRGIDPAREGTVTELAHNLVNGNLDDLARQNKVVIPEKEREPNGS
                     DTAMRPGIILGKELSMRLGAFPGDTLNVVSPVGPVTGASMTPKIRQFVVVGIFQSGMY
                     EYDSSLAYIELGEAQKFFNMGASVTGIEVKVTDVFRAADIARAIEHHLGFAFWARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQREIAILKAMGATRK
                     GIMRIFMLNGLIIGCSGAAIGVPLGYTFLWLIQTFWTFDPTVYYISRIPVHVLGSDVL
                     LVAGSAILISFVATLYPSLQAAKLDPAAALRYE"
     gene            19658..20326
                     /locus_tag="PK224_05695"
     CDS             19658..20326
                     /locus_tag="PK224_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB15261.1"
                     /translation="MIDVVDLYKSFPMGGRELVVLNNINLHIKRGELIAIMGASGAGK
                     STLLQILGTLDRPTKGTVSFDGQNLFTLTEQEQAEFRNKRVGFVFQFHHLLPEFSALE
                     NACLPAMIQKRDMADIVGEATKLLGEVGLADRLHHKPGELSGGEQQRVSVARALMQQP
                     DLVLADEPTGNLDSHTGDALFTLLRQLNRSRGTTFVIVTHNDKLSSQADRIVSMQDGM
                     IVSS"
     gene            20468..21613
                     /gene="traF"
                     /locus_tag="PK224_05700"
     CDS             20468..21613
                     /gene="traF"
                     /locus_tag="PK224_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HRB15262.1"
                     /translation="MKTSFAPYVRLTTLCLAILLPLQAAAVEFVFVGSRQMGMGGAGV
                     ATTSDSLATYWNPAGMAMSKKFDIRFQGSGQVVDRGDVFDSLKDINNLNLNDTSAGNI
                     ARLQQQIDRINRPGTNLTAAASGGLYVKGNFGEHALGFNVSDVATAGGFLRGPVGFTN
                     NGTNLSVNGQFAMNGLEVRQAALSYAYAFMDRMFSIGVTGKVIQGAAYTGSTNIRGAS
                     DDVRVFEDIGRAKISTAIGIDVGAMFRPSSWLRMGIVAKDINAPTFDAPNGDKFKLLP
                     QVRTGVAVNPYNSLTLSADVDITKNNTLTPGVYSQVLSLGAEQTILSELLSFRVGAFK
                     NMQDAKTPFIPTAGFGLRILALRIDIAGGYDFRDQAALASGSVALTF"
     gene            21660..22265
                     /locus_tag="PK224_05705"
     CDS             21660..22265
                     /locus_tag="PK224_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15263.1"
                     /translation="MTRHTVRTISVVFCLVMTGLLTGCGGLQEMWEGPAARGFYPKTM
                     AILPISGTYDSAREDVEEVVAKVLIKSRRVEKVVPPDQVTDTFQTTKDAFDALVTYFS
                     RMETTGQSDKNSAIKIGHALGADSLLVVKVNAWEYTREEGDNIARVGLSMRLVDAVNG
                     AIVWKARHQVQESYLFIRPDMRDLATKLATDMIKYMPPEKR"
     gene            complement(22268..23341)
                     /locus_tag="PK224_05710"
     CDS             complement(22268..23341)
                     /locus_tag="PK224_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRB15264.1"
                     /translation="MAVARRKVSRTNRKTTSKTASVAPRRKATARVPAAMGEERIIRT
                     LQQGLFDTLSGSEGEGRSPISVSAAFVQSFLQLTKVQAIAVYVRDEQTREMTCLAEAG
                     SSDASVAGKWREGLARAEQNAQVSSGDLYGLRLHRIGRMDGVVMFQTGKSQRLPSRKL
                     LTVLTAVEPWLAVLLDHARLTVKYAAKILRIQHMEQVSDLLNSSLAEEEKLRRALDAA
                     VRLVEAEAGALFLSAGDGALTLCTVAGERVTGLSALHSSIATGVHRTGQAVLITRGEQ
                     DARLVAGQGWQAALPVASLVSVPVRMGTQAVGVLEVVNRRSGKPFSNWDVLELASLSN
                     QFGLAIDNLRRGTVGEGGPKRVG"
     gene            complement(23544..24344)
                     /gene="trpA"
                     /locus_tag="PK224_05715"
     CDS             complement(23544..24344)
                     /gene="trpA"
                     /locus_tag="PK224_05715"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRB15265.1"
                     /translation="MNRLDHTLGQLKAKGEKALITYIMAGDPSLQETEQLVLELERAG
                     ADVIELGVPFSDPIADGPVIQKAAERGLQSGTSLRRILESVRHLRTSTQIPIVLMAYY
                     NNIHAFGEAEFCRIAADAGVDGLIVPDMPPDEAGPLRGPAGEAGLHLIFLLAPTSTAS
                     RRAYVAKESGGFVYYVSITGITGAKLQDVAGVRENVAKIRKHTKTPVAVGFGVATPED
                     AARVAQVADGVIVGSAIVRRVGEHGQDGRLVQEVGAFVRSLKEAMQPG"
     gene            complement(24510..25700)
                     /gene="trpB"
                     /locus_tag="PK224_05720"
     CDS             complement(24510..25700)
                     /gene="trpB"
                     /locus_tag="PK224_05720"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRB15266.1"
                     /translation="MAIPDRHGRFGPYGGRYVPETLMPALLELEEVYQRTRRDKGFQA
                     ELKHYLKEYVGRPTPLYFAQRLTKRLGGAKIYLKREDLCHTGAHKINNAIGQALLARR
                     MKKPRLIAETGAGQHGVATATVAAMFGLECEIYMGTEDMQRQALNVFRMRLLGSTVTG
                     VDAGSRTLKDAISEAMRDWTTNVRTTHYVLGSVLGAHPYPMMIRDFQSVIGREARKQI
                     LAAEGRLPDCLIACVGGGSNAMGLFHAFVPDKKVRMVGVEAGGLGVESGKHAARFAGG
                     RPGVLQGTMTYLLQDENGQVNLTHSVSAGLDYAAVGPEHSYYKETNRAQYTSVTDDEA
                     LEAFDLLAREEGIMPALESAHAIAEVVKCAPTMKKSQILIVNLSGRGDKDVQQVARIK
                     GVTL"
     gene            complement(25748..26380)
                     /locus_tag="PK224_05725"
     CDS             complement(25748..26380)
                     /locus_tag="PK224_05725"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRB15267.1"
                     /translation="MAVKVKICGLTNAEDAAVAVEAGADAVGFVFHKKSPRCAEPAAV
                     KAIVKELPPFVLPIGVFVNEDAKVVRDIMDSCGLALAQLHGDETAAYCEMLGRPVLKA
                     IRLRDRCSFLALAEFQGRAGVRGFLVDAFSPDAYGGTGQVADWALAAEAATVARILLA
                     GGLTPGNVAQAIEQVRPYGVDVSSGVEASPGRKDHDKVRAFVQAVRLVSR"
     gene            complement(26385..26699)
                     /locus_tag="PK224_05730"
     CDS             complement(26385..26699)
                     /locus_tag="PK224_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15268.1"
                     /translation="MKTRLSLGVMAALLTLAGCMNQEIHEYSPKWDAWMGSSKDDRIK
                     DMGIPTKCHSFKDGGEVCEWSVPQQDGRQDLIGLTFNAKGQACQWSYRGFYGMQKSKQ
                     SC"
     gene            complement(26696..27520)
                     /gene="trpC"
                     /locus_tag="PK224_05735"
     CDS             complement(26696..27520)
                     /gene="trpC"
                     /locus_tag="PK224_05735"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRB15269.1"
                     /translation="MILDRILEHKKAEIRHKNSRGYLADLKTRIRDAGPTLGFAVNLD
                     ARRTRTRPALIAEVKKASPSLGLLRPEFEQRFEPVAIAEAYREHGAAAVSVLTDKEFF
                     QGSLEYLSDVKRKVGLPALNKEFMVADIQFYEARAYGADAVLLIVAGLEKRQLIDFSA
                     LAKELSLDVLVETHHERELDTVLEWLPDVRLIGINNRDLKTFSTDLGVTLRLAKRIPG
                     DKLIVSESGIHKREDVTRLVEAGVHAMLIGESLIRAQDIGVKIRELLGDESNKEKP"
     gene            complement(27525..28541)
                     /gene="trpD"
                     /locus_tag="PK224_05740"
     CDS             complement(27525..28541)
                     /gene="trpD"
                     /locus_tag="PK224_05740"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRB15270.1"
                     /translation="MIKDAINTLAERSDLTEQEAETVMGEIMDGAATSAQIAGYLMGL
                     RMKGETVAEIAGSVQAMRARATKIRVRDSQVVDTCGTGGDRAHTFNISTTAAFVAAGA
                     GLTVAKHGNRSVSSKSGSADVLAALGVAIDLPPERVADCVNEVGIGFLFAPLYHSAMK
                     HCAQPRQELGIRTLLNILGPLTNPAGARIQVVGVFDAGLTELLAKVLLHLGAQHCFVV
                     HGMDGLDEITVTDRTRISEGKAGVVSSYTIDPTEFGLTRVRPKELVGGSAEDNAAITR
                     DIFRGRKGPKRDIVCLNAAPALVAGRKAKTLQEGFQLAQQTIDSGAAMEKLDQLIAFT
                     KKAS"
     gene            complement(28628..29191)
                     /gene="pabA"
                     /locus_tag="PK224_05745"
     CDS             complement(28628..29191)
                     /gene="pabA"
                     /locus_tag="PK224_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRB15271.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVVVYRNDKISIQEIEALKP
                     RRLVISPGPCTPNEAGISVEAIRHFGGKLPLLGVCLGHQSLAVAFGGEVIRAERLMHG
                     KTSMVRHDGRTIFRNLPNPFEATRYHSLIVNRKNLPDCFEISAETAEGEIMGMRHKML
                     GIEGVQFHPESILTTAGKELLRNFLKL"
     gene            complement(29282..30778)
                     /gene="trpE"
                     /locus_tag="PK224_05750"
     CDS             complement(29282..30778)
                     /gene="trpE"
                     /locus_tag="PK224_05750"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRB15272.1"
                     /translation="MSRQQYSLTLDEFRALAAEGNLIPLYREILADYETPVSAFAKID
                     HGPTAYLLESVAGGENWARYSFLGSGSSAVIREEKGDLVLIRGKKNLRIQSRGNPLER
                     LRELMEEYRPVTVPGLPRFVGGAVGYFSYDMVRTFEDLPALRKDSLGMPDFAFLLTDT
                     LLIFDNVSQKIKVVANAYLESTKERDIRDAYRHATARIEKMIARLKRPVRQPRQRRRR
                     KPITFTSNMNKADFEKMVTRTKEYIRSGDIVQAVLSQRWETQIHTTPFQLYRALRVIN
                     PSPYMYYLRVAGVELVGSSPETLVRCEDGQISLRPIAGTRRRGQTPEEDQDLARQLLA
                     DEKERAEHVMLVDLGRNDVGRVAARGSVKVESLMQVERYSHVMHIVSQVTGQLEKGKS
                     VYDVMRACFPAGTVSGAPKIRAMEIIEELEPTRRGPYAGAVGYFGFSGNMDMCINIRT
                     VVIKGRQAYIQAGAGIVADSIPEHEYEETCNKARAMMKAIELAEQGLE"
     gene            complement(30799..31497)
                     /locus_tag="PK224_05755"
     CDS             complement(30799..31497)
                     /locus_tag="PK224_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRB15273.1"
                     /translation="MMSQRNSRMRRAVVVPIGLLVLSGLNLGGCVMSEKYEAEKARSL
                     NFQRLLAQEEKRSAELDSEVKRSKRELSEYEARNRELGAQVEAVRQQAAQIQEEAQAM
                     KEASLLEKRAHEDMKRLTTIPSKTKKAAAAAKKDFTAAVDEALKTDVSSELSLESAKD
                     AAKDTFGHEQAAVDALGAASASATGNTHTVRSGETLYRIGQKYHVGVDQLRKWNNLDN
                     NTVVVGQKLIVSRP"
     gene            31735..32688
                     /locus_tag="PK224_05760"
     CDS             31735..32688
                     /locus_tag="PK224_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB15274.1"
                     /translation="MSPNTQPGTSRTVTIEGITLHLAQPMTMDQEWIGNREILKQLLA
                     CWLVIDERDLPLSPRITGQPGIGKTTLAMAGARERKQDLYVFQCTADTRPEDLLITPV
                     LAESGTISYHASPLITAVLTGSICVLDEGNRMNEKSWASLAPLLDHRRCVESIIAGIL
                     INAHAEFRCCVTMNEDASTYEVPDYILSRLQPTLGMGFPAREDELAILRYHLPFAPAE
                     MLAMTVDFLQEAHQLSLEYSVRDGIHLLQYALKRRAQDPTHPLSADAAWRESLMKVLG
                     EEALDLPTQSRRRKRALGDQALPQGLGDFFFESDDPLHPGR"
     gene            32688..34586
                     /locus_tag="PK224_05765"
     CDS             32688..34586
                     /locus_tag="PK224_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15275.1"
                     /translation="MAVSSSTADSVFRLSPRIELFPLLHGSGDVAQEVRERLTDRRFD
                     CLAVPLPPSFEHPLEEAVMDLPTISVIVQPERDQEGAATVNYVPVDPCQAVVMGIRVA
                     MGEGIPRAYIDRETAVVEPVPFVSPDAYALKRLSYATFAAAMVPTLTPPAEGSQQQAR
                     IAWMAFQLHQLEMEYEQILCLCHFADWPWIRQAYQQRTDYQIPALDFGRPERYLVEPA
                     SLYFVLGELPFLTELYERRRATVHSDRHLSIDGIKELLLETRTRWQTGHDAEGQTVPT
                     WVTPQLLQAYLQYVRNHALLDRRLTPDLYTLVLAAKQMAGDDFAVRLLETAKSYSYQE
                     IEQSHVPHLSAGLGRFALPDEQIVRAKNRLQGTPLAWRSLSLRPRPPRRTSRQWSYLW
                     NPHRQCSWPPEDSKIESFHTHVREQAKAIIGADLVKSEKFTTSMKDGIDIRESLRHWH
                     QRQKPGRPQRMEIYVKEIPPARGNVDTVIFLFDTPGDPEQYSWQATWYAEHAQESTLC
                     FYATPFLNNMIAPGIGQSRYGGALFMFPPRPIPDIWTDEALGFARTLEERLIAAGAVH
                     SRETHVALVTPVPPKARWRQIARQFDRRLVPIPLSRFSGQMIDRLRRFHVLNGHEIRS
                     FAAQFIRE"
     gene            34594..36606
                     /gene="ligA"
                     /locus_tag="PK224_05770"
     CDS             34594..36606
                     /gene="ligA"
                     /locus_tag="PK224_05770"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRB15276.1"
                     /translation="MPHQTAEQRIADLRRQIRRHDHLYYTKDRPEISDAEYDRLFREL
                     VDLETAHPDLVTGDSPTQRVGAPPLDELIKVSHEKPMLSLDSITDQDDVRAFDARMKR
                     ELETDQIVYTAEPKFDGLSVELVYDRGAFVRGATRGDGMVGEDVTINLRTIRALPLQL
                     DPQPSLPAHLVVRGEVYMRLDEFQVLNRRMTERGEEAFANPRNAAAGSLRQLDSRITA
                     SRPLTLTCYDIMAISGTAPPTHWEELDALAAWGLPVPEHRQRCRSIEEVLSFHAGTDG
                     MRDSLPFEIDGIVVKLDRRTWQDQLGSKSRSPRWAIAYKFAPRKEITVIQDIAVSVGR
                     TGTLTPLALLKPVEVGGVTISRATLHNADEVARKDVRVGDTVKVERAGDVIPAIAERV
                     PVPGETRQAPFVMPDHCPVCGSAVAREGAYYYCTGQTVCVAQLKGAIEHFASKSALDM
                     DGMGKKTIAQLVDVGLVRDLADLYSLTKEQLLSLEGFADRSATLLMESIERSKSTSLD
                     RLLMGLGIRQVGQHIARVLAKQFGTLAKLMSATQEELLQVREIGPEISTSLASFFSEE
                     RNRAVIARLIERGLTIAPPSSEIAAGSQSLAGKTFVFTGGLAGYGRDQAKQLVEQQGG
                     QVSSSVSKKTSFVVAGTDPGSKLDQARKLGVRVLTETEFTALVTPE"
     gene            complement(36581..37894)
                     /locus_tag="PK224_05775"
     CDS             complement(36581..37894)
                     /locus_tag="PK224_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRB15277.1"
                     /translation="MDIIVLIVLILLSAVISVVEVGFYSVNDTKLRALADTGSKRAEM
                     ALHLRTDPQRLLLTILVGDRLIDTATASLATIIALNRFGGQGLEGVLGEAFAVLVGIL
                     TFVLLVFADLVPKTLAAKYSVPVVLNMAYPAYAAQQVLTPIMFFVVPLIYKLTGGKGL
                     NVPFVTEEELKIMLDQSSKSGAIEAQEVKMIKNVFQLKDITAEDCMTPRIYMFSLDCN
                     QYLREAKELLFKSKYSRIPLYEGTLDNIIGILYKTKALTALAQGHTEMKLRDIAQPAL
                     FIPHTKSADDLMKQFQLDKRHMAIVVNEFGGVMGLVTLEDLLEEVVGEIVDETDITEE
                     LIKRIGKNQILVHGRTEVRKVNDFLKVDLGDDAVTISGLVQHELGRIPKVGEEVHIAN
                     CRLVIHEADPRVIKSVNIYKEEKHPITHEPIVDEHVSVTQASLER"
     gene            complement(38162..38869)
                     /locus_tag="PK224_05780"
     CDS             complement(38162..38869)
                     /locus_tag="PK224_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRB15278.1"
                     /translation="MPEEKRTSTSRLLLPLFAFLLTTWLSGCSGWSPARPSYPPGYPL
                     GFIERGSASWYGPGFHGNRTANGEVYDMHKLTAAHRTLPLGSVAVVRSLSTGRQVTVR
                     INDRGPFARGRILDLSLAGAQAVGMVGRGTDEIELRVISYEGRAGGMGVLRVQVGSFA
                     DPANARTLVERLRSAYPGSKIVGVDLPDGRRYRVYAGQFQTEVAAEQAAAHLRRALET
                     DPFIVRDDAPGATTGNP"
     gene            complement(38878..39288)
                     /locus_tag="PK224_05785"
     CDS             complement(38878..39288)
                     /locus_tag="PK224_05785"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRB15279.1"
                     /translation="MPTTTQFVVSGQSKPGVLAEVAAVLGAAGVNIKAFSAPEVTGPG
                     KLRLIVADVDTARIALRKSKIKFREETALILSLENKPGALKQVADSLTRARINVKCGY
                     CTPSREGKRAIVVLTVSNTTKALGVLRTHSLDEF"
     gene            complement(39328..39600)
                     /locus_tag="PK224_05790"
     CDS             complement(39328..39600)
                     /locus_tag="PK224_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB15280.1"
                     /translation="MVDQRAVAREWQARGFSCDLWVDPPGQVWEDYRHATDELVMPVE
                     GRLELEFGGRCVRPEPGEEILIPAGVSHTVRNIGGTSVKWLYGYSR"
     gene            complement(39623..40735)
                     /gene="fbp"
                     /locus_tag="PK224_05795"
     CDS             complement(39623..40735)
                     /gene="fbp"
                     /locus_tag="PK224_05795"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HRB15281.1"
                     /translation="MAAESTQSITLSVIKADIGGFVGHSAMHPALMDCAKEKLAVAKT
                     SGLLVDYHVSACGDDLQLIMTHRQGVDHEGIHRLAWDTFEAGTAVAKELHLYGAGQDL
                     LADAFSGNVRGQGPGVAEMEFVERKSDPVLIFMADKTSAGAWNLPLYKMFADPFNTAG
                     LVIAPTLHQGFRFEVHDIYEHKKISFDCPEELYDMLMFIGSPGKYTIKYVYSRADGTI
                     AAASSTERLSLIAGKYVGKDDPVMIVRAQQNFPAVGEVLDPFRYPWIIEGWMRGSHYG
                     PLMPVSVKQATPTRFDGPPRVACLGFQLADGTLVGPRDMFDDPSYDEARRDANRIADV
                     LRMHGPFEPHRLPLDDMEYTTMPQIMKKLEGRWAKV"
     gene            complement(41062..44604)
                     /locus_tag="PK224_05800"
     CDS             complement(41062..44604)
                     /locus_tag="PK224_05800"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRB15282.1"
                     /translation="MRIERRFTKRGQSPYEGLAFVKRSSEIRNPDGSTVFKLDHIDIP
                     EHWTQLAIDILAQKYFRKAGVPQVHEDGTPVVDAAGKPVLGGERDSRQVFNRLAGCWT
                     FWGKNHGYFKTPEDATAFYDEMCYMLAFQMAAPNSPQWFNTGLHYAYGLSGPAQGHFY
                     VDPKSGEVVRATNAFEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGSGTGTN
                     FSRLRGDGESLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDIEE
                     FIDWKVVEEQKVAAMVTGSKICAQRLNAVLKACLLVDAQGLGQVELDMKKNQVLRDAV
                     TAARRDMVPEAYIHRMFDYAKQGYTHFVFHEYDTNWDGKAYQTVSGQNSNNSVRIPNG
                     FFDALERDGDWELRRRIDGKISKTVKARDLWDKIAWAAWICADPGTQYDTTINEWHTC
                     PEDGRINASNPCSEYMFLDDTACNLASLNLSKFFNVEGEFDLDSFRHAVRLWTVALEI
                     SVLMASFPSRAIAQKSYEYRTLGLGYANLGTILMKQSIPYDSPKATAICGAITAIMTG
                     ESYATSAEMAAEIGPFPSYNKNRESMLRVIRNHRRAAYSAPHEEYEGLTILPTSIQPE
                     HCPPAMLLAARRAWDRALELGAAYGFRNAQVTVIAPTGTIGLVMDCDTTGIEPDFALV
                     KFKKLAGGGYFKIINQSLPPALQALGYTDAQIRDIGAYCAGHQTLKGAPFINHEVLRQ
                     KGFDDAALERLEGSLAQAFEIQFVFNKYTLGEDFCRQKLGISEAQLADPTFNMLKHLG
                     FTQEDVAAANDYCCGTMTVEGAPHLKLEHLPIFDCANRCGRIGQRYIAVDAHIRMMAA
                     AQPFISGAISKTINMPADATLDDVKAAYLFAWKSMVKAVALYRDGSKLSQPLNASSDS
                     GKGAEATPSVMTVAEKVAERVLVRYLHKRRSLPARRSGYTQKAIIGGHKLYLRTGEYE
                     DGTLGEIFLDMHKEGAAFRSLMNNFAIAISLGLQHGVPLEEFVEAFVFTRFEPNGPVK
                     LNDRIKMATSIIDYIFRELAITYLERTDLSQVREEDLRMDSMKKDEQDPECVDEEADM
                     TALAKSSILTEHLPVRRNGMNGGNGHSHRPGNGSVAHKVELKRETVTVTSVRQEAKEK
                     GYEGDPCQNCKQFTLVRNGTCLKCMSCGETSGCS"
     gene            complement(44923..45294)
                     /locus_tag="PK224_05805"
     CDS             complement(44923..45294)
                     /locus_tag="PK224_05805"
                     /inference="COORDINATES: protein motif:HMM:NF025629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4359 domain-containing protein"
                     /protein_id="HRB15283.1"
                     /translation="MSNVTLAGLVGSLLVAVGLAATNPTMDAYVHFVEARLLAEIEKM
                     DQSGPRHDRDVIKAVFRAQGPKLVEGVVRPNTTRNNWGLLSFFETNVLDQPVLVLGVA
                     GQFVPLRGVEEATLKVGRLAF"
     gene            45537..45827
                     /locus_tag="PK224_05810"
     CDS             45537..45827
                     /locus_tag="PK224_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15284.1"
                     /translation="MFVWSKRLVTALLAGFVLLIGVFLIQGEPESASGLKIHTTRWMA
                     VNYIGLLVWIFIWMFDQARVRGKNVLLWLLPFALAPLPTLMLFILFLQRKVK"
     gene            complement(45944..46162)
                     /locus_tag="PK224_05815"
     CDS             complement(45944..46162)
                     /locus_tag="PK224_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15285.1"
                     /translation="MRAEYDFSKMKGQRNPYRGQLKQPITIRLDKATVAYFKGLADEI
                     GMPYQNLINLYLRDCALSQKKLDLRWAS"
     gene            complement(46166..46450)
                     /locus_tag="PK224_05820"
     CDS             complement(46166..46450)
                     /locus_tag="PK224_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRB15286.1"
                     /translation="MAGIRFEWDEKKNRHNIRKHGVSFEEAQSVFLDDHALRFFDPDH
                     SEDEDRFLMLGLSFTLRILVVCHCYRERDGLIRIISARKATRQEGEHYER"
     gene            46560..46763
                     /locus_tag="PK224_05825"
     CDS             46560..46763
                     /locus_tag="PK224_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15287.1"
                     /translation="MKIHTTRWMAVNYIGLLVWVFIWMFDQARVRGKNVLLWLRPFAL
                     APLPTLMLFILFLQRKVKQEGAL"
     gene            complement(46803..48068)
                     /locus_tag="PK224_05830"
     CDS             complement(46803..48068)
                     /locus_tag="PK224_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB15288.1"
                     /translation="MNRATLRAVLTPRLGIMLPLGFASGLPLALTGGTLQAWLTEAGL
                     DLTTIGLFAYVGLPYTLKFLWAPVMDRIVPPWLGHRRGWMIVTQTGLALALTLMAFIG
                     PGSGAQIFAALALGVAFLSASQDIVFDAYRTDLLKPDERGLGAATWVMGYRIAMMASG
                     SLALIVAARLGWSSAYLCMAGLMALGIVTILMIPEPEAARAAPQSMSEAVWGPLSEFL
                     SRPMALALLGLIVLYKLGDAFAGALTTSFLLRGMAFSSEDIGLVRAFGIGATILGAFI
                     GGGMMPQLGLFRSLMVFGVLQALSNLSFLWLAWVGKSYAVMAFAVGFENLTGGMGTAA
                     FVALVMSLCNHRYTATQFALLSSVEALGRVFLGWPAAKLVGLAGWGPFFFVTFLAALP
                     GLWLLWVLRKPVTQHAELNAGREGVEVPC"
     gene            48422..49189
                     /locus_tag="PK224_05835"
     CDS             48422..49189
                     /locus_tag="PK224_05835"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HRB15289.1"
                     /translation="MLSDLEHIQLAEEKVRRFITLAEEILRLHDKAEQRINEGYRNEY
                     FKPTLRNRVIAGLAVKAGRSFERLIVDARNRRGECAHHLKTMVECFIYACWVSGDETE
                     KRASLLQAESFRSRAAYHENCSASPEDEDAHWAKEWRELQHKQIKGIEKEWKNFPSLE
                     SIAADVKSKDLYNIYRASCEAAHTGDLPLYMPPQPTEPGLTLADLSLQQCYVSLQSGV
                     FLACELLRNASDQVELELESEIDSFAQRSREIRLLGT"
     gene            complement(49260..50114)
                     /locus_tag="PK224_05840"
     CDS             complement(49260..50114)
                     /locus_tag="PK224_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRB15290.1"
                     /translation="MTVEPTPAATNPVADAYPPPEIAARVCKVGLAKVTTPVPTMIAL
                     AVLAGAFISLGALFYTVTITSGKSGPALPFGLLRVAGGVTFSLGLVLVVVGGAELFTG
                     NNLIAMAWAVGCVQTRQVVRNWVWVYLGNLLGAGGTAVLVLLAGVQTLADGAVGETMV
                     QIARSKIALDPVSAFARGILCNVLVCLAVWLCMGARSVADKILAIVFPITAFVACGFE
                     HSVANMFFLPLGIALAAGGVAPLSVMDAVTNLLLVTLGNIIGGTVLVALVYWFVYLRT
                     EPAGRSNP"
     gene            complement(50111..52834)
                     /gene="fdhF"
                     /locus_tag="PK224_05845"
     CDS             complement(50111..52834)
                     /gene="fdhF"
                     /locus_tag="PK224_05845"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRB15291.1"
                     /translation="MKLEINGRLVLASPGDTIYGAAKKAGIAIPGLCSSDHLAPFGSC
                     RLCLCEVEGQSGLPASCTTPVREGMVVHTESERLTRLRRNIVELYLSEQPAGDRAPES
                     LQWLAKSLGLRQVRYPQPSHRLDVVDRSNPFFTFDNAACISCARCVRACDEIQGTHAL
                     TMLHRGFASRPVAGAASLTGEAAGFASSNCVSCGACVKECPTGALMEKTVAEQGTPER
                     TVRTTCAYCGVGCAFNAGVRDSTVVSMVPADDGPSNQGHACMKGRFGWTYNYAPDRLR
                     VPLLRQGDKWVEISWPDALDRIAHEWVRLKDIYGSDALATISSSRGTNEENYLFGKFM
                     RCVVGSNHIDNCARVCHSATVTGMMDTIGASAATNSIQDLDLAKLILVVGANPTESHP
                     VVGARIKQAVRRGAALIVIDPRRTELARLADLHLQMHPGTNVALLNGLGHILVKEGLI
                     DQDFVGDRTQGFEEWVKVVESCTPETTSKVTGVPAHLIAEAARRYATSGGSMAVHGLG
                     MTEHRWGSHGVMALVNLALATGNLGKPGTGINPLRGQNNVQGASDVGCLPTYFAGYQP
                     FDSPELAAAHLAITGRPLPTTRGMKTPDMWDAALAGTLKSLWIIGYDVAQTDPNLKKV
                     HAALKNLEFLIVQDLFLSETAKLAHLVIPGASFLEKDGTFTNLERRIQRIRKAVEPPD
                     GVLPDWQVVCEVSARMGYPMWYGHPSAIMDEIAQLAPMFAGVSYDRLEAPEGLQWPVP
                     SKEHPGTSLMHERSFPKGKAQFVAVDYLPPGESPTETYPLVLITGRILQHYNCGAQTR
                     RTEIMQVVDTDVLEIHASDAARLDLHEGEIVRLVSARGEAKLPVMVSERVQPGELFTS
                     FHFPDTDLNVLLSSSADESSKCPEYKVSTVRIEKILPAGTPSTPMRVMLIT"
     gene            complement(52950..54446)
                     /locus_tag="PK224_05850"
     CDS             complement(52950..54446)
                     /locus_tag="PK224_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRB15292.1"
                     /translation="MRTVTRLYLSNDTSARAAGAGVLADAWSEHPEVQLIRISSRGAF
                     YLEPMVERDSPAGRVAWFNVTAQDLPLILSGTDGTPVRSIPFLAQQTRFTFANFGETE
                     PLALDEYQARGGLSGLETALRIGPDAIIEELQVSQLRGRGGAAFPVWNKWKVAQQARA
                     DQKYVVANADEGDAGTYCDRMILEGDPFRLLEGMLICARAIGAGRGYVYCRQEYPAAA
                     KTLRAAIQKADEAELLELDGEPFPIEVVEGAGSYVCGEETALLESLEGKRGVVRARPP
                     YPAQSGLYGRPTIVSNVLTFATIPNILSRGGAWHAALGTEQSRGTMALQLGGRVKHPG
                     LVEVPFGLSLHQVLEQFGGGMAPGSRFKAVQVGGPLGSLFPASQMDIPICYDAFAKAG
                     AVLGHGGIVVYDHETDMVDLARHFMAFTADESCGKCTPCRIGSVRGREILERIQAGSG
                     TIEDLSLLHDLGDTMKVASLCALGGRAPYPVLTAIEHFFAEFRSKLRT"
     gene            complement(54448..54897)
                     /locus_tag="PK224_05855"
     CDS             complement(54448..54897)
                     /locus_tag="PK224_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRB15293.1"
                     /translation="MDEQVKSILETVRGEPSNILKALLALQEQLGYVPAEAVSEIAHA
                     LGVTTAQVAGVLSYYPDLRLTHPGRHLIRVCMGESCYANGCGRLLRELQDRLRVDVGE
                     TAAGGKFTLDTMSCAGNCAVSPTVMIDRDLYGRLLPSQLDTLLERYK"
     gene            complement(55105..56475)
                     /locus_tag="PK224_05860"
     CDS             complement(55105..56475)
                     /locus_tag="PK224_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15294.1"
                     /translation="MKLANERVVGRSVPVTKELDIILMRLDRLSMTPGFAQQRRIAFT
                     RALQVYVELGSQAPLSPLPEEVGLADLLLYADFYPEDGQLTLIEQLRDVITEHIPEEE
                     RVWLDPLKHSYMDLVELLPQEIGKERRNLRSIGNGRVYEVPADSLGSDAESGQVLFTR
                     LVREPGDPESPLAVPAGPVLMLSAEDAKALYETTGEERREMEVVGGSFELGDWPEFAK
                     RFGHLLLRNFARMRLAALVEAVSEIRYRTTAGAPYFYVMALYEHREFTFIAGGMAELE
                     GWKEEAPVRSAGATASGKLRRFIQGSSGAEAGASPTARVTVTATELFLECDSRERLDT
                     VKHSLAAAFGFSLHFRGEAVQPPMRQVTQAELSAAEPLTVVVTDEEDRTLMNAFLETV
                     YLEWADRESPAVGGETPRHVMPTQSGRTQVAALIDEMERNDFGLLRTGVAAFDYNKLR
                     AHVGLC"
     gene            complement(56459..56683)
                     /locus_tag="PK224_05865"
     CDS             complement(56459..56683)
                     /locus_tag="PK224_05865"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB15295.1"
                     /translation="MKPKVKKPSAPQSKVRAVSSAVTNPIELPEGMWERISQKAFELW
                     RERGCREGHALQDWLDAETAVMEEIHEARE"
     gene            complement(56712..57647)
                     /locus_tag="PK224_05870"
     CDS             complement(56712..57647)
                     /locus_tag="PK224_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB15296.1"
                     /translation="MTLIKRVLFATDFSACADRAMGYALAMASAWKAELCVMTVLELY
                     PGMDPDYTVNKMYLDHLRAEASRQLAAVEARAKAAGQPVTVRIETGIPSQAVQTVAHT
                     IGADLLVVGTHGRTGLDHVLIGSTAERVVRMAPCPVLAVKADKGSAAVPAMANIKRIV
                     VPIDLSACSLDALEYAVQFAKPFGASITILHAMEPVAYGLDFSLSHAKEWKEQRAYLE
                     KRLTVLVACVQAQGLQVDHVLKPGLPADSIASYVTQQGYDLMIMGTHGRRGISHVLVG
                     SIAGAMLRHAPCPVLTVRQFNFGANYQRVVPLGEA"
     gene            complement(57651..58079)
                     /locus_tag="PK224_05875"
     CDS             complement(57651..58079)
                     /locus_tag="PK224_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRB15297.1"
                     /translation="MGVRKVSDLLSGGRVWLAACFVLALCSQGGAQAEQRIEVTIKDS
                     VFVTKQVPLRLGVATVIAIVNQDQVRHDFGSTMFDGIPTRVESGGIVSYGKQIGGLFL
                     DAKKEAVVRFTMERPGRHEFRCSIHPNMKGELLLLNVEAV"
     gene            complement(58092..58592)
                     /locus_tag="PK224_05880"
     CDS             complement(58092..58592)
                     /locus_tag="PK224_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB15298.1"
                     /translation="MADRLFTKILVPVDFSPCSEEAFRLALSFAKSYQAEVLLLHVVD
                     TKSLDALNRLGLAPATEAAKQKKQLHHFARLNARQLLARDDAKGVTIRRLLADGCPFE
                     EIARTARVEGVDLVVMGSYGGAIGGVDKIFFGSTAEKVVRTAGCPVLTVPLPTKRAKV
                     KTGKSL"
     gene            58979..59074
                     /locus_tag="PK224_05885"
     CDS             58979..59074
                     /locus_tag="PK224_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HRB15299.1"
                     /translation="MSRTQWLLFGAVLVGAAVALYLIFFCPTDCH"
     gene            59139..59813
                     /locus_tag="PK224_05890"
     CDS             59139..59813
                     /locus_tag="PK224_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRB15300.1"
                     /translation="MEAESIFQVRISHDGITLDGILGLPAHPRGVIAFAHGSGSGRFS
                     PRNQFVARHLETEGFATLLMDLLTPDEADDRRKVFDIDLLADRLLLAERWLQAHRQTA
                     GLRVGYFGASTGAGAALQAAAREPQAVGAIVSRGGRPDLAGPYLSRVTAPTLLLVGGD
                     DGPVIEMNQDALRQLTCRKELVIVPGASHLFEEPGTLEQVAREALRWFQRYLQPDAHA
                     AKESRS"
     gene            59810..60673
                     /locus_tag="PK224_05895"
     CDS             59810..60673
                     /locus_tag="PK224_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB15301.1"
                     /translation="MKVLLAVDGSDHSYEAVRALKYLRRPDELTLLHVVDTPRPAYPM
                     MVPEVAQELYSQLERSMKEDGEQLLTRIHSLLPPHSGPVTKQLALGSPAEMIVATAES
                     RHVDLIVMGARGLGPVKERLLGSVSHRVLSFAPCAKLILQGSLRDLKQVLLPLEGQSD
                     AEAALRFLRKQPFHEPVNINLLAVLPPTRPPWPVDDSVAEKLETQALRHARDFVDGVA
                     TELRTLGYTTRSASVLGTPVTMILHEAEKMGVDLVMVGSRARQGITRFVLGSVSHAVL
                     HRAPCQVLVFE"
     gene            60741..60896
                     /locus_tag="PK224_05900"
     CDS             60741..60896
                     /locus_tag="PK224_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15302.1"
                     /translation="MMNVSLERFQVRNNNKESHHNPQAGQKGRPARPQGGVRSRTPVA
                     VFLNSLA"
     gene            complement(61070..61477)
                     /locus_tag="PK224_05905"
     CDS             complement(61070..61477)
                     /locus_tag="PK224_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15303.1"
                     /translation="MTNLRVAASLLLAVLLFIPATSAWSQDPLPIEPDLNSRLDELYD
                     HEARLFIMLYSLHGDGKVDYITGRLVQEYTRSNYGNPVYYTEPYPLFYWWDHTMFNDP
                     DQDGVNGNERVYQENIEFDIARYKPCLFNGQPC"
     gene            61634..62965
                     /gene="mtaB"
                     /locus_tag="PK224_05910"
     CDS             61634..62965
                     /gene="mtaB"
                     /locus_tag="PK224_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRB15304.1"
                     /translation="MPRASLHTLGCRLSQSETSMLADTLARRGYQLVEFGKETDLLVL
                     NTCSVTENAEKDCRYAVRKTLRHSPHAFVAVTGCYAQTGATQLQKVPGIDLIVGTQFK
                     MNLPDYLPAPAKLLKQPEPELRHTRTIDREDFVLPGTAYSDSTRALLKIQDGCDFMCS
                     FCLIPFARGRERSRTAEDVLREARELAAHGYRELVLTGVNIGQYSYQGLGLVELLREL
                     DAIPDVTRIRISSIEPTTVPAALLEHMAGSTKLCHYLHLPLQSGDDEILQAMNRRYAV
                     REYEELVEQAIALMPDLGLGTDLMVGFPGEDEQAFANTVRTVERLPFSYFHVFSYSAR
                     PGTAAARLEDQIPPAVIRQRSKALAELSRTKALAFYQQQIGRTLPVLFEQGERDGFRT
                     GTTANFTRVAVAADAVEAGSIHQVTITGIMDGLAYGRPVATVLAPSHRPLV"
     gene            62962..64320
                     /gene="miaB"
                     /locus_tag="PK224_05915"
     CDS             62962..64320
                     /gene="miaB"
                     /locus_tag="PK224_05915"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRB15305.1"
                     /translation="MTQPNKPHTVHIETFGCQMNEYDSELVRSLLRKAGFEFTEDRER
                     ADVMLMNTCAIRENAHNKVYGHLAELKAVKEQRPLVVGVLGCMAQNLKEELTEKQPLV
                     DVLVGPDGYRQLPGLLTNALNAEEQGLVQRGMAVDLSEYETYDDILPERDDSSNAWIA
                     IMRGCDNFCSFCVVPYTRGRERSRDPQGILREVEASVATGHTQITLLGQNVNSYRYED
                     WDFARLILAVAEVPGVRRVRFTSPHPKDFPAALLDAVAGHPNICKHIHLPLQSGNDRI
                     LELMNRTYSRKEYLDLAAHIRRRHPGIALTTDIICGFCSETEEEFLDTYRVVEEVQYH
                     SAYVFKYSERKNTIAARKFPDDVPEAVKGERVSRLVDLQRPITARLNRELIGQTLPVM
                     VEGDSKRSSDQWMGRTDTGVYVIWNKSDAPALLGSIQPVTILDGSAAVLMGRREPSAR
                     VA"
     gene            64773..64904
                     /locus_tag="PK224_05920"
     CDS             64773..64904
                     /locus_tag="PK224_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15306.1"
                     /translation="MKKDIAQSHAEEAPRGMSLETIIAVGVFGWMALGVVMMGLGIW"
     gene            64990..66036
                     /locus_tag="PK224_05925"
     CDS             64990..66036
                     /locus_tag="PK224_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15307.1"
                     /translation="MRIHGSRTPFTKTLNSGLLGLTLLGAMAAGCSSTAATVQQKAAA
                     QPQGSVRLEEVADWEFEASHPSTIALATLDSLLRGVMISDAPMDLSNLPVDGSKPMKV
                     FSDEDVHYLAPLLAQALSQAQPEYVVAFRLSSSAGSGSEPTAGTLYVQNERLYLTVTE
                     HNGTLAKVESSLFSSGRPARIVSITPESAGKIEQALPAIAQGRQPLKSLAIDYTLFAK
                     NPEPAPAVAGTIAAPAPQPVQASAPRMVVASVVEEAPVKNAPPAQPAGPASHASKASS
                     AEDERLTETLQELQDAREAMARKDAKISALRRDLESMRTQLETKDKELRAVKSTPQTQ
                     PKRETRNKAELTVR"
     gene            complement(66053..66769)
                     /locus_tag="PK224_05930"
     CDS             complement(66053..66769)
                     /locus_tag="PK224_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRB15308.1"
                     /translation="MKVVLDIETVQAPREEWARLAGRQLASGEAAFSETGGNLFAVGE
                     AQAQHRLDEELYEKSSFDGTFSRIVCIGLLEFSDSLEPRGATSWYGANEQELLRRFWS
                     HLGQLRPSLFITHNGLNFDLPFIKKRSIIHQVKPTMEINLAKFRAEPVYDTMAIWSNW
                     DNRGWVKLDVLARALNVESKSGSGSQVAQMWADGQGKEIALYCLQDTYVTYGCYCRMN
                     FRQPISREIVLLRPEVIDVG"
     gene            complement(66776..67171)
                     /gene="mutT"
                     /locus_tag="PK224_05935"
     CDS             complement(66776..67171)
                     /gene="mutT"
                     /locus_tag="PK224_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HRB15309.1"
                     /translation="MMEVIEVAAGIIVHEGRYLIARRKAGVHLGGLWEFPGGKRETGE
                     TLEECLHRELREELNVRIGLPTPFQVVRHEYPEKIVELHFFCCRIETGVAIALDCAEL
                     RWVYPHEMAAFEFPSADQPIIAALQQGKV"
     gene            complement(67289..68011)
                     /locus_tag="PK224_05940"
     CDS             complement(67289..68011)
                     /locus_tag="PK224_05940"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRB15310.1"
                     /translation="MSTKSRVQKSPRRKKKSSQSSVPLARGLKQRFQNRLLKWYKEHG
                     RDLPWRRTSDPYHILVSEVMLQQTQVDRVIPKYHEFLERYPSFEQLADAPVAEVKQTW
                     YPLGYNIRPERLHSIACETVARYGGQLPNDAEELLSFKGIGRYTAGAIRSFAFNEDAP
                     ILDTNVIRVLHRVFIAQGDPKAQKAALWELSETLIPRGKGYDFNQALMDFGATVCTAR
                     DPYCLLCPMKPFCKTYPFDPAR"
     gene            complement(68164..68529)
                     /locus_tag="PK224_05945"
     CDS             complement(68164..68529)
                     /locus_tag="PK224_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15311.1"
                     /translation="MNASLFICPVAVSGVLVAAMAFGNPAMLPQHPGYPMGKSIDPIH
                     GQSLANDPGRANAVGESALSKAAAFDNRHVSQQLPKDEQNQRLLEKPGAGILPKVQGP
                     QIVIDPPVKEGTKVQASPQ"
     gene            69023..69346
                     /locus_tag="PK224_05950"
     CDS             69023..69346
                     /locus_tag="PK224_05950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB15312.1"
                     /translation="MTMKVHYQVSLSTESATSGEGRLLDLSVEGCRIECAHHLPVNTY
                     LSLRLIVSPKEMPVLVDLAAVRWTRGIVCGIHFLSLQPLQTARLKTFLASAAPQKPPN
                     TPDKR"
     gene            69487..70104
                     /locus_tag="PK224_05955"
     CDS             69487..70104
                     /locus_tag="PK224_05955"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HRB15313.1"
                     /translation="MNEENQTRRIDVLAIGLFGLAVGALTLGVAQLGWIQHKNMVGAL
                     VIALIFGGIVQLLAGITDIRYNEQLGGTALTMYGFLWITLCTVKLVGTSGTLQFDAVL
                     YAPINLVYAVFSGVMIFLTAYRNLTLSALHVVITLTFLATTFARLDFISELLPGWGHI
                     IVGLMAFYHAVGSLTQAFTGKSILPLGPPLLFHTQKHVHSIAKVV"
     gene            complement(70395..71114)
                     /locus_tag="PK224_05960"
     CDS             complement(70395..71114)
                     /locus_tag="PK224_05960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB15314.1"
                     /translation="MKRLNGKVAIVTGSSSGIGKAIALRFAQEGATVVVAARRMYKCE
                     ETVAQIEAAGGTAVPLQTDVADESQVERLIGDTVRRFQRLDILVNNAGIFGGRRLAET
                     STEAFDEVMNTNVRGTFFCCRAAFAQMKKQGGGTILNMSSVAGVQAWSGTGTYSASKH
                     AIMALSKALADEGRAHRIKVSAICPGGVADDLVDASAGERAGSEKIDPFDIAETALYL
                     ACLGPQSAVHQIVVDRIGADW"
     gene            complement(71272..71541)
                     /locus_tag="PK224_05965"
     CDS             complement(71272..71541)
                     /locus_tag="PK224_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15315.1"
                     /translation="MSNDTKDIRITGCDVHVTFHAAGDGRWSADATVGCGVGHNLAEQ
                     SVASGPCSTREAAEQEVLARVGEVLGRNVDRHTSRVENPTEKNPR"
     gene            complement(71688..75227)
                     /locus_tag="PK224_05970"
     CDS             complement(71688..75227)
                     /locus_tag="PK224_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB15316.1"
                     /translation="MKLARLLLLAFGPFTNKTLDFSTGSGNLHLIYGPNEAGKSSALR
                     AMTDLRFGIPLRSPDDFVHPAGELRIGGVFIDQTGRPVGLIRRKGRGTTLSGLDVRTE
                     QTDPGFAVDSRLERELTGGLERREFEAMFGLNHGRLREGGAVLLSGEGDLGSALFEAS
                     AGTSGIAALLEALDADAKKLYSQHGRAQNAVINEARRQLDEQRKAWREAQTKPAEWQA
                     LNRAHETAKAALDDLTKALETLRRRENELTELRTVEPLLREHDRLAGALQSYAAVPDL
                     SEQQREERLAAEQALQRAQVDLREAEEELARCTAALDGLVIEPLLLDHADAIERLVSG
                     VEAAARNRHEVHQQNSLIAKIEGELDLVVARLAPGVDRRTILAAVPSVADRVALEGHL
                     AEVSRLGERLEGYRERAEALDAALQPASALPDTVPDPLHRQALTAALRAGQAQGDVVR
                     QKSELDRRLRELEGRLTLALSELGLESEQALRRSQPLLEAQIAQAKQDLTDIEVQLTK
                     WRDESELVGRDLDGQRLRQRQLAAEGEVVTAETLRQARAKRSEEWTAIRRTYIDRTGG
                     TDQLALGFDATKALPETYEAAVGEADRQADLLRADTKRAAGLEECSVRIEQMELRRKE
                     LAGEMAALRAKRDGLQGAWRQRLTQAGLPDLDPETLREWQGRRHEALQLVERVTALRA
                     DRDRLLADAAGSVGAIIEGLRAVGCSPAATQAGEAEQLSALIEQAVRWEQSATEAEAE
                     QQARLKTAHSQRIEREKVGRQVSETEAERRRHLDGLQSWHTRLFLSGGAPPEALKSRL
                     DELDGLARQASALAEAQQRKAHLQAVVDDLMTQGAQVAELLGEAVPTVLDDFADRLRK
                     RLAVSRQHQQEGHALIRDRRKAQEKKRQAAAAQVTEAAVLAGLCTRAMGATIDQLPEL
                     EEQAANKREVRKSLALLRQQLAQASPQSEAELRARLTGLDVPALESERERCRTERARL
                     EQEQTSAQRTEEQARRALEVIDASDRAALAREAMESAAARYRSAIRPWARLKLARALL
                     QEALNRFRERAQAPMVSAASAYFSLMTGGAYERLVTDEREDRPVLCAQRAGGVTIGIE
                     EMSEGTADQLYLALRLAALELRRPSHPPMPLVLDDTLITSDDARAANILRALARFAEG
                     SQVMLFTHHRHLLDLARGTIGDHAFVSHHLGDT"
     gene            complement(75224..76483)
                     /locus_tag="PK224_05975"
     CDS             complement(75224..76483)
                     /locus_tag="PK224_05975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRB15317.1"
                     /translation="MRFIHASDLHIDSPLRGLDRYDGAPVERLRSATRSALERLVDKA
                     LAERVDFLLLAGDIYDRDWQDFHTGLFFRGQMVRLERAGIRCFIVQGNHDAQGVISRQ
                     LTLPSNVTVFSSRAAQTVKLDDLSVAIHGRSFPEREVNEDLVPSYPPPVSGYFNIGLL
                     HTSLTGRAGHDTYAPTDLPTLVAKGYDYWALGHVHAREVLNERPRIVFCGNLQGRHAK
                     ETGVKGCELVTVEAGRIEAEFIALDVVRWSQLSVPLDGVDRLESLNEAFARALEPVLD
                     GTTDRLHAVRVTLTGSTELHRLEAAQPGTLAAAMHAAAQDIGTAEIWIEQVRLDLSTP
                     LDRARAAQRQDAVGELVRLVDTIAGNEQELMRRAQVELGDLLGAMPAEVTAGDVPRLD
                     DPAELRSLLMDAEATVLARLSASGEEA"
     gene            77147..>77529
                     /locus_tag="PK224_05980"
     CDS             77147..>77529
                     /locus_tag="PK224_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRB15318.1"
                     /translation="MNLESDPSTALSPEAIAQLVKGHREFLAFLERRVESRAVAEDIL
                     QTAFTRGLERGGDVKDEKVVAWFYRVLRNAVIDHYRQRSTTARAMEAWGREFPDVQEP
                     EAELRQEICRCVSGLLETLKPEYREA"
BASE COUNT        15735 a        24921 c        21486 g        15387 t
ORIGIN      
        1 cggaggaatc acggtaggcc gcatgtagcg tgaggacgga tcttgcgacc ccatcggcgc
       61 ggtgagcgcc ggtccacccg tcatgccggg cagccccttg ccgatcgtgc cgaacgactt
      121 gccctgactg gagggaatgg tgttactttg cggcgcgctg gcgccggaag ccgtggccgt
      181 aatcgacgac tgctgcgcga ggggattttg gagcgggagg gttcccagtg attgcgggtt
      241 ttgtccgggc tgattctgtg gggtcgatgc cggtggcgaa gtctgggccg tcacgaacga
      301 cggggccagc atcatggcca cactgaggcc gagggcacag atatttacgt gaccgagtct
      361 tcgatacgat ctgccgtgcc gactcaccga gactcccctt cttcaattac accagctgcc
      421 tgcaaggcca gctgaatacc gtaatggagg gcttctgtcc accgggacgt tgtagcacct
      481 catcacgcca cgcatgaacg gctcgttcaa cccgctcggc atgcgcatcg tccacggcga
      541 ccatgatcac attgttgacc cccggataga aggagacacc gtcagcaggt cccgtctgtc
      601 cggctccggt cgtctcgggc atttcggtgt aggcgcgaac atcgcactgt tcgagtaaca
      661 catgcacccg ttccttcaat gacgaccgga aggccaacat caacatattc atatcgtgac
      721 tccaggaacg taacgaggct cgttcgacgt gaggcccggt tggaggcaat gcggagttcc
      781 tccgctaatc caaccagtcc ttttccttca gcttgcgcgg cagatacttc ttcgtcaggt
      841 attgaaagtc tttgttcgcg agcgcgtcca gttcgtactt cagcccgttc gcgagcatcc
      901 gtttgatttc ctgctgccag gccggcttct ggaaccactg atagttcagc agctccttcg
      961 cgcgggtaat gtctttctcg ttcagcttga tgcccacatt acgcggcaac tcataccgct
     1021 ccggatctgc actggaaagt ccgatgaact tcgctttggg aatcgccatg cgctggctct
     1081 caaacgcgag gttgatcgag ccctgtttca caaccgaata aatataatac ccccagggat
     1141 cgttgtcgac cagcacatac acggggagct tgtgctcctc atgtaggcgg cgcgccagcc
     1201 gccgcacccc gcgcggtggc tgcccgttcc ccgtcagaag cacacagttg tatcgccgcc
     1261 agaacttatc ttccgagagc cggttccact gcgtgccttt ttcaaccagc aggagaaaat
     1321 cggcggtaca gcggcggatc tccagatatt ccggttcgac gatcgagggc acagaatatc
     1381 cgcccttgcc cagcttggca caatccaccc ggtcaccgtc gtccccgaac accaccggcc
     1441 cgacgatgct gccgctgttt tccgcgcgca cgtggagttc ttcccggagc gcgaccagcg
     1501 agacttcgag atcctcgata atgggatcgg actcgtcctg tgtatcgaag gtattctcat
     1561 gggagtcctg aatcgtatgt ttcgtacgat aatagatttc cctgagcgag gtggtcagat
     1621 cggcttgttg caactccgcc agcgcatcgg ccaccagcat cgtctgcatg aatttcttcg
     1681 ccatcccgac gttaaaaaac gagcgggatt gcttcttctc gcccatctcg atcagccccc
     1741 tgcggggatt gaacgacacg ttcgacaacg cgcggattgg aatctcaaac gtcgggtcct
     1801 tggcccgttg cgccgccgca atgaccacat cggccatccc gacaagcttt ttccccacgg
     1861 ccccgttctt cttcgtcttt gcctgtgcca taccctgtcc tatttccttt tccgggtggc
     1921 ggatacctgc gcgggttttt tcttgagcgg tgccgactgt ttcgttgacg gctgctcatc
     1981 ggcaaacaag gcttgctgcc cgactttcgc cttcgttgcc ccgacagact tgcccttccc
     2041 gaccttcagt gtctccttcg agccgcgctt cgccttcacc ggatttttcg tctcggtgat
     2101 cggctcagcc tccgccgttt tggtcgcagg cgcaggcgat ccaggagtct gctgagccat
     2161 ctcggtctct ccctcgatcc cctcggcagt cacgatgatc gaatgcggca gcccttccgg
     2221 tcccgcgccg gtctttccca gggcctcatc ggtcttgagg ccccccgtcc gtgaactggc
     2281 aatcttgtgc aactgttcct tcaatttctc cgtgggaagg tttccgcctt tcaggcgatt
     2341 gcaggcggcc acaacctcct cgatatagag atcgaagata ttgcggcgac ggaactcact
     2401 ggcagcccgc tctcttcttc gcagaaaaat ccccagccgc cggccggctt cacgaagcgc
     2461 gagcgtgatt tccttctgaa tttcgtcgta gtcggcaata gcttctttcg actcgctggt
     2521 gaacggcacc cacacggatg ccatgtgcac gaagatcacc atcggcccgg caggcagcgc
     2581 gccacgggac tgcgacaccc catagtttcg ccaggtggtg ctgagcaccg ctttgaacgt
     2641 agcgcacgcc gattgttgat agagcaacgg cacccgattc gcgtagcgga tcacccgcgc
     2701 ctgttcggta tcgttcccct ggtcttcacc ttcggccaac ggcacggcgg cctgctgcgg
     2761 cttggccgct tcgtccggtc ctttcccgaa ggctaggccg gcctcgatga taaagggata
     2821 gccgcgatag acggccggtg gacgactcac cgccgtataa aactcacctt tgatttgctt
     2881 atagaggccc gagaggatcg ccttctcccc gatcggcgag atgcagttcg tcggcggcgc
     2941 catgatcttg gtggtctgaa tcgtcttgta gagcgtttcg gccgcctgat gcttcacatc
     3001 gcgaggcttg gttgcgggcg acactttcgc cgccttgcag atctcctcgg ccaacgcagg
     3061 cgaaacccgg cagaagtcgc tcgcgaggaa accggctacc gtatggctct tcgtgtcctg
     3121 caacatcttg agcaaggccc cgaactcgat gccataggga tgcggcttga tttctttcgg
     3181 agagggcggc agctcgtgat acgtccgtgg atactctttc gtgtcgcctt ccggggtgtg
     3241 atagacgagc cgcacatgcg ggttggcgat cgacgtctgc tccagccact catcgacgga
     3301 cgcgcggccc ttctggtaac gcccttcgac ctcgagcgcc acttcggtgc cttgcggctg
     3361 agcccagtcg atctgcttgt tctcatgcac caacggctcg ttcttcttcg tgtcgatttg
     3421 cacttcgaaa tagtgcgccg ctgcgcgagg ccccgtgcgg gaaatcactt tcaccggctt
     3481 gccggtcgtc aattgcccat acatgccggc cgctgaaatg ccgatgccct gttgccctcg
     3541 gctcatccgc atgcgatgga atttcgagcc gtacagcaat ttggcgaaca cccgggggat
     3601 ctgttggcgc acaatgcccg gcccgttatc ggtcaccgtg atccggaaac gagtcgcctg
     3661 gctgggcgca accggcgcct gcccgttcgc gaccgtttca agcctcaccg tcacctcggg
     3721 caggatgcct gcttcttcgc acgcatccag cgagttatct acggcctctt tcacgcaggt
     3781 cagcagcgct ttgcggggat tgtcaaaacc caggaggtgc cggttcttcg taaaaaattc
     3841 ggagacggaa atttcccgct gccgcgcccc catttcgacc gcggtcaccg cagcggacga
     3901 ttcttctcgg gaggtggatt tttttcgtga cgcgactgat tcagacacgg tggtttcgga
     3961 ctggggcgca ggagcggagg actgagcacg tggcgatgat gtcttggtgg ccattcaacc
     4021 tctcacgcaa ggaattcaca ctggaacggc tcgcttagat tcacttgacg aatacgtacg
     4081 ctcttcgtcg tttacaggga acgcccagga gagtcaagcc ttgagcgtat tctcgtcggc
     4141 atctcgatgc aggaaaacac ggtgcaacgg tggacgaaat gcggggtgat cgaaccggga
     4201 gtgatgagga gcgacgatgc agggtggtga cggaggtttg ggtgggagga ggacgcacct
     4261 accgaggaga ggtagatacg gcggagatct cgggaggatt tcaggcttcc cagtacaagc
     4321 tgggcatgca caccaccctc aacactcaat cccctttttg tacatattcc cccggggcgt
     4381 taactccccc cacccggtta cgataggcct cctcacgtcg atgagtcaag agcatggcag
     4441 gcgatttacg gaccgtccgt gtcggcctgt cgatgtgtga tggtcgggtg atggagccag
     4501 gccgcctcga tccggacctc cgccccgatc ggacagtaca acgacactga tgtattgtta
     4561 ctctcgacca gcgctctcaa ggaaccgcgt tcgttgtcga gctccaacac acccagatgc
     4621 tcatggagat ccaccacgca ggtgatgctc cgggactcgc gatcactcac gatcgtcctt
     4681 gccaccaccg gctcttgcag cgtgagcagg tcattcggcc ggaaggtcgg ttgatccttc
     4741 atctcgatgg caatcaacac gcctgccccg gccaggagca gacacacccc gaacaaaaca
     4801 gcattggccc gctgcggata atggatcttc aaccaacgaa cagccgtgcg gatgccaatg
     4861 accaacagca gaacgatgaa acccagcgcg acgacggtca acacaagccg aaagttcatg
     4921 cgatcccttg tgcctggttg acggcaacca ctgaatacag tccgttcgtc gtcatcaacg
     4981 gagtcagcgt catcctgaat ggaaactccg tgccgtcttt ccgtaagccg acgcattcac
     5041 gaacagggct caccgatcgg tcgtccgggc ggatcaaaaa aacgccagcg cggtctgagg
     5101 cttgcgggcg cagtctcgcc ggcatgagcc gttcaacagg ctgaccgggc agttctcctc
     5161 gctcatagcc gaactgcgtg tccacatggg gattcgtcag cagaatcatc ccttccgcat
     5221 ccaccacgat gatgccattc gaggccgatt ccatcaccag gcgaaggcaa tccgcgctct
     5281 cacgcaacgc ctcttcggtc cgtttccgtt cagtgatgtc ctgcgcgaac accaagaccc
     5341 cgagcacatt cccctgcggg tccaggtacg gcaccttgtc ggtttgcacc cagcgttttt
     5401 caccgtcccc tgtctgatag ggctcgatga tcccgagctt cggccgccct gaaacgatca
     5461 cctccagatc atcctgatga taccgctcgg cctcttccgg ataaaattca tcggttgacc
     5521 ggccctcgat ttccgccacg gttttcttga tggatgccgc cgcccggcga ttcgcgcgca
     5581 ggatccgatt gtgcgcgtcc ttgtaccaga ccatggccgg gatcaaatcc aacagcgcct
     5641 gctgctcaat ctgctgtcgc aacagtcgcg cttccgcctc tttccgcgcc gtgatgtccc
     5701 gcacaatgcc gcagtgcacg cgctgaccgt cggagatcca actgctcagc gacatctcaa
     5761 tcggaaattc acggccatcc ttgtgcaacc cgtgcatggt cacgaccgtg cccttcaggc
     5821 gtaaatcacc cgtggtacgt actcgcgcta acgcctgtac gtgcctggca cggtaccgtt
     5881 cgggcatgat catcgtcagg gactctccta acacctcccc ggccagatac ccgaacagac
     5941 gctcggcggc gctgttccag gacaggattt ttccctcccc atccgcaagc acaatcgcgt
     6001 cgggcgcggt ctgcacaact tcccgaaacc ggtcttcgct ggccgtcaac gcctgcttgg
     6061 tcgccgcagc ttcgatggag agatgcttca ctcccacggc acgccggatc agcgctttta
     6121 attcttcggc atcgtaaggc ttcgtcaaat acccgaaggc tccttcggtg agggagctgt
     6181 gctttttcgc cacctcgaca aacgccgtca gcatgatgac cggaagcacc gggtcgattt
     6241 gtttcaggag ggtgagaacc gagagtccgt ccatatcagg caacatcagg tccagcacca
     6301 ccgcggacag agattccgcc tttgccttgg aaagggcctc ggcaccggtg ccggcgactg
     6361 agacacggta gccgacgtgc tccaggagat catgcagtac gagtgcaata tcggggtcgt
     6421 cgtctacgat caggattttg ggaggtggaa gctcggtcac gctgggcgtg gtctttgaca
     6481 tagcggtcaa cgaaaccatg ggtatcgtca tacgaccggt ctgacgcttc ggctgtcatt
     6541 gtcctctttt cgatgacaag aatccagatg tctagtgagc cgctgctgaa tggctcatcg
     6601 gataggcaca ggaaagacct cgtccggcct tcactggtcg attacccgat gaaccatttc
     6661 acctcagagc gcctcacttc ttccggtaga cattcaggcc gattttctcc tctcgccccg
     6721 gaatggtgac atttccctcc gtcgaggcaa taatcgtggt tttccccgag gctgatggcc
     6781 cgaactcttt cgacaaatcc accttgatgg tcagcacggt cccctcgacg gtcatctcta
     6841 cattcttcat gttgctcccc taatccttca ggacgaatgt gaccttcaac gccacacgat
     6901 actgggtgat cttcccgtct tcgatatcca ctttctgatc ctcgatccag gcgcttttca
     6961 cattctgtaa cgtcttgttc gcgcgagcga ttccaagatg aatggcatcg tcaaaactct
     7021 tgggggatgc agcaatgatt tccgtcacac gtgcgacaga catgatagcc tccctggttt
     7081 gaatggtgga taccacactc ggcacctcag gctaggccga ccataaagcc ctgtcaagcc
     7141 cgcatctcat tgaacggccc gccggcagtg acgcgtgaag atgacggctg aatctcagta
     7201 gcctgggaaa acacccaggt tgacttgtta cccctcggag gatagcgtgc cccacatcga
     7261 cggtcgtaac acccgtatgg gctcccctcc atgccgaccg agatcgagat ccaaatcacg
     7321 cctcttttct cgacgcctct gctggtgttc acgcctgcgc atcacgaacg catcaatgcc
     7381 gagctctctc aactgattct ggcgcgcgag gcttccgtcc cgagccaccg agacaccgaa
     7441 gtgatcgggt ggtcctcgcc tcacgacctg tcgatgatgg attgggcagg aagccccctg
     7501 cgcgagttgt ttgcgcccgt catcgaagta gccaaacaag tcacaaccct ctccgaccga
     7561 tgccgccatg ctgcccgtcg acccgattgg gaagtcgtcg aggtgtgggc caacgttcaa
     7621 cgaaacggcg ggaccaatgc agcccactct caccccggaa gtttttgggc aggcgtctat
     7681 tatgtcgatg tgggcgaggt ctgcccgacc ctgggaaagg gaggcgaatt gcagatttac
     7741 gacccgcgag gctgtctgcc gcgtatgctg gcgccctatc tccagtacag catgaccgaa
     7801 ttacatgatg ccggaaccag catttcctac tcccctgcgg ccggacaatg cctgctgttc
     7861 cctggctggt tgttccatgc cgtgaatacg tatcgtggaa cggcgcccag aatcagtgta
     7921 gcgttcaatc tcgatcccgt cttacagcag ggccccctga gcagcgctca cgcggatgcc
     7981 gtcggcggat cacgccggtg agagccccct cgccgcagcc tgcgcagctc gcacgcaggc
     8041 gatgcgcatc gtggtctccg gctctcgcta tggcagcgcg ttcgcctgaa tgaagagagc
     8101 ctgctcgagc gcgcgaaaga attgctgata cggctccggg gactcgatgg ccttgaggta
     8161 atactgcgca ctggcacgca cgatcacctg cctgtttccg cgtggccgga cggtcaccgt
     8221 gaggcggaca agattgctgc ggtgataaaa cggcccccag ctccggaact gccgggtaag
     8281 gatcaggagt ccgtcgtccc gatacaggtg ataggaagga tcaccccatg gcaaggattc
     8341 gtagtcatca aattttttgc ccgagacaat gcccagagtt tcatccaaca cctccaccgc
     8401 aaaacctaag tcctgacaag tggcaacaac cccctgaatc gtcttgagac gatcagcggt
     8461 atcgaagacg cggctttgag ccgcccgcac cttgacctgg ctggcctcgg caagccagat
     8521 ctggtcgcgc gactcccatt ggttctcatg tcgccactca tagggcgaac agccggcagt
     8581 caaggccagc aaggcgaggc caaccacgac catgcagttg ttcgaccggc ggcatcgata
     8641 catggcgggg cgagccaccg actagtcctt caccagaaat aacgaacgtt ccaccgccgc
     8701 gaagaagttc tgatacacct tgggatcttc gatcggcttg ctgttgtaga tcgcattggc
     8761 acggatcatc gtcttgccgt cctgttgtgg gcgcacggtc acggtcacgc cgagcacatc
     8821 gtaatagttc ggttcggcaa accgggccgc cgtcaccagc ccgagcggtt cgttcgcgcg
     8881 ttcgatgata aatcccaggt cttgcagagc cgcgatgacg ccgcgcatgg cgacggtctg
     8941 ctccttgacc tcgaacgtgc gggtctgcgc gctgcggatc ttcatctgcg catcggtcgg
     9001 cgcaaagagc tcctgcctcg gctccggcgc aacgcagcct tgcaagatga cacttcccat
     9061 catgaccacc actgcgccga ctagctgtgt ccagttcatg aatcctcgta attaaatcga
     9121 atgcggctcc aggaacacag ccttggagag ctttgcaaaa aattgctgat agatctcagg
     9181 gtcccggatc atttccattt tctgttcacc gggtggaatg tagttgcggt cggcctgccc
     9241 atcccccttc cacaccgccc ggtagaagac aatccggact tcctgccgcg aatgtgcctc
     9301 gttgagcggc cgcgtcacga ggctggccgc aattttttgg tgcaggtcga tcgggatgac
     9361 gacgtgcccg agtgacagca gccagataaa gaaccggttt cggtactgac cgtactcacg
     9421 agcgctacgt tccttcgcgg ctcgcaggaa cccgacttcc cgcacacttt cctcgacttg
     9481 aaacccgaga tcctgcagcg cggcagccga ggcagacaac aattcctgat cgttctccgt
     9541 gtcgaagaaa cgggtctgca tggcccgatt ggcgctgctc tccggcgtga gttgaaacaa
     9601 ttctgccggc tgcgtctgtt gcacgcagcc ctggggcaag accaacagcc ccatgagcag
     9661 aagaacggcc gcagggagcc gctgcatcaa tcgaccggct ctctcagaat tgggtggagt
     9721 tgtaggcaaa gtcccggacc ttcttgtctt catcgtactt gatgataatg gtgagggtgc
     9781 gctgacgctg cgaactggac cgatcgctgg agttggctcc gaggatgatc aatccggcat
     9841 aggaagaact ggccgtatcg acacgatccg tggacacctt gtcgtaaatc cacacttcac
     9901 ggcgcttgtc gtcggtcgtg acgatattcg gcgaacccag cagttccgcc acctgcgccg
     9961 ccgacatgcc gactttcacc tcgccctgga cccgcccgac cgtcaaccgg tcttccttga
    10021 tctccgcttg cctaccgccg cacccggtta cgcccgtcag cacacagagc ccgagcactg
    10081 ccactcccca tcgcatgtgt ctcatcgtat acgtctccct tcctgacatg accatgtctg
    10141 caccacgccg ttgggccatc ttatcctgcc gacaacctcg tggcaaagga cgatctgtgc
    10201 gacggaatca catcgctccc ctcagattgc tggacaattc accggaggcc gctacaattg
    10261 gctgatcagg aggtactgcg atgccgatgc cggatgccat atcgaatgat gatgttgaag
    10321 cagcgtggag aacgttggtc gaacagacac gaacgggtgt gttactgaca ctccggaacg
    10381 ggcatccctt cggctcccat gtaccctacc tgctcggaga cgactgggct cgcgtgtatc
    10441 ttcacctcag ccggctggcc ttacataccc agcatctcct tgaagacgcc cgtgtcagcc
    10501 tgtttctcgc cgagccggac aagccaggca aaaacccgct ggccctgcaa cgcatcaacc
    10561 tgcagggaac ggcctcaatc ctccccccga ccgacatggc ctatgcatcg gtgaagcagc
    10621 gttatctcgc acagttcccc cagtcacaaa tgatgttcgg tttcggcgac tttgccttgt
    10681 gggagctacg gatggaggat gcccacctcg tgctcggatt cggacaggcc taccaggccc
    10741 gttcaaccac ccccggacag tggcagcatc agaagcccga caaaaagtcc tgacccggct
    10801 cattcgcggc acccgcccat catcggccaa tccctctgcc gaacactcct cgcccctcca
    10861 aagcattagt cccttcgccg aaggaccgat ggtgattcac gcaccctgca cagtaccctg
    10921 cgcaaggtca cgtgaactca tcgaaaggac tccccctatg ttcttcatcg tcgccgtcat
    10981 cgccggctat atgttgttac tggtcggtct tgccgtcgcc tcgcccggca tcggattcac
    11041 cggttcgctc ctacacctta tgccggatga ctggcgtgat gcggcgcacg tgccggccta
    11101 tggcctcctg gcctggttgg ctatgattgg gttccgcctg cgtggctggc cgattcgcta
    11161 tgccctgctc gttggaatcg cgtttgccgg ggtctttggc ttatggacgg aggtggcaca
    11221 ggggacggcc ccgggacgcg aagcctccct ccatgatctt tcgaacgatc tattgggtgc
    11281 catgatggcc ggcgccctga tgctgtggca acacagcacc tccacacgga ctgatcagtt
    11341 cgagccggct ctgagagcga acctacccca cctgaggaaa ggaacgtcgt cgcgatgaac
    11401 actgcctatc gtgcgcgcgg ggccaggcgt attcccgttc agtaccctat ttactattca
    11461 aacggcacat ttcaaaccat cggcgtcatg gaggacttca acgcctcagg ggggcgtatt
    11521 cgcggcaaag agtcggtcag cgtgggaatg gagctggtgg taatcgtcat ccagccgacc
    11581 cctgaaaccc ccatgctcat ccgccgggcc acggtccgct ggtcaaaagg ccacgacttc
    11641 ggcatcgccc tctcgggcgt tcccccacaa tccgaatccg agctcaaggc catggccaaa
    11701 gtgatgctgc ccggcctctg gtcttgtctc aactagaagg ggcttcacca gctcgggagg
    11761 tcctcagcac cgaatgggag cgccacgccg ttccctcgct tcgagacgga gtcctcttcg
    11821 acctcatttt cttcgcacat gatccagtgg ctgccgcgga ccggcaaaga acaactccat
    11881 caacacaaaa aacatcatga ggagtgcgcc cagcccgatg gcgacggccc agttttccca
    11941 cgacatgtct gacctcctct tgcccttgaa aatcgtcgaa cgattcccag ggggcagcgt
    12001 tacctgcctg aacggtgcaa ctgcaacaac gtctccagct tccacagcgg aatactgtcc
    12061 acctccctct tgctgctgaa cgccaaaccg cccacgaaca atgcaatcag ccccagacag
    12121 gccagcacca aggccccgcg cgaaatatcc gcagtggcag tcgagtggta tgcgactatc
    12181 gtgcggccgg ccggcatctc cttgagcaga gccaaacgat ccaccaatgc atccgtgcga
    12241 acggccgcac tcatcgcact ggcgagttga atctgcccgg ccgccttcgc ctccgcatac
    12301 ccttcttgcg cctgcgtcac ctgaatgacg gcccgcccga ttcgctcctg gaccgccgcc
    12361 gccataccat cctcctcctg aatcgcctcg actatcgagc ggccaagtgt cgcctgcctg
    12421 gtcgattcaa actggtcgtg catgcgctgc ccgatcgatt cggcccgctg aatcaggttc
    12481 gtgttgaatt cgccggcata ctgatcggca gagaggactc cgcttgcaag accgcggcga
    12541 gtctggttga cgacggactt gcccatgaca tactgcatgc gcgcacgatg atcggcttcc
    12601 tgtccaaacg cgcgcaactc tgccaacacc agcggactca gcaacgtcgt cgagatcagc
    12661 tgagactcac tcaccgcccg gtttaactcc atggctgaag cagagagggc ggcaccggcc
    12721 tcccggtcga gcaagacatc gtgaagaatc gcctggccta aaaccggctg cagatactgc
    12781 attccggtgg aggggtcgtg agtcacgatg gtcatattgg cagcgggagc gaccggcgcc
    12841 tgccagaaac catcggcagc cgtcaccaat aggtacccgc cgaacagcgt cgccaccaat
    12901 ccgacggcga ccaccacatc aacgaggtca tattttcgtc ccatgatatc ctcctatcct
    12961 gcctcgggta gttcggcgcg tggcaaacag gcaggacgcc ggcgcgccgg aatctgagcc
    13021 gcaccagatc gctgtcgttt aagcatagag acctcttcct tggtgtgatg gagcacttgc
    13081 agaatggaaa tatattcccg gccttgcgag gcacaaagcg cgggctgcag atcttgatgc
    13141 ggaacgtcgt tgatgaagga taccggccgg aggagacggg acaaggctgg cttggtcaga
    13201 gcggcggtca tgttcgccac ggaagaccat gcgaccacct gcagaaaggg tggcattgaa
    13261 tgacttagtt agtctatgcc tcgctcaaaa agcagtcaat acggcaagaa ctaagagccg
    13321 gcgcgggttg ccgggcatgt gctctcccgc atcattcaac atttgaaaaa cgtgattcac
    13381 ctctcaggaa tagacatgca gtacaatgtg gaaagagatc cctatgcgta tcttcgagag
    13441 ggagtgtcct atgccaacca ctagcctgct gatgctactt accgcatgga cgctattagg
    13501 cccggtttcc atcgctggct cccagccatc gatccttcaa cagcgggacg ggaccaccag
    13561 ccccctgact ccgctgggtg agcaagacgc catctactcc gacgcgcatg gaaacaaagg
    13621 tgtcacccat ctggggtccg gccttccgtc acacacgttc agttctcccc atggcgcagt
    13681 gcccggatca gtcacaccat tcggaacacc gacaccggcg aatctcgtca caccggcgcc
    13741 gttactgccg ctccaaccaa gaggtatggc gactccatac ccacatcccc ccgcatcatc
    13801 cggtagtcct ggcggataca gcaggttcgg cgggcgatcg gggcgctaac cggtcgaacg
    13861 accagtctgg gatgatcctc ccccaagccc atccgccaaa gagcacggtt gaatctcttc
    13921 aggtctttcg ttaagttaca ggcaatgacc atctcccgcg cacgcctcat tctgcttgcc
    13981 tgcgcgagcg gtcttctcta tcccctctcc ttccccgcgt tcgacctcgg catagtggcc
    14041 tggttcgcgc tggtcccgct gcatatcgcg gtcgaatacc tgtcgccggg tcgcgcgttc
    14101 cgtatcggct ggctttccgg cacgctggcc ttcacgggct cgatgttttg ggtcattacc
    14161 gccatgaacg tctacggtaa ggtcccgttc ccgattgcca ccctggtgat gttgttgctc
    14221 acggtctacc tcggactcta catggccgtc tacgcaggcg gactttcgtg gatccggaca
    14281 gcgttcccga ccctgggctt cctacccgcg ccgtttctct gggtgacatt ggaactgatc
    14341 cgtacctact ttctctccgg cttaccctgg gcactattcg gatattccca ataccaatgg
    14401 ctcccgatca ttcagctggc cgaccatttc ggggtgtacg gcgtctcctt cctcctcgtg
    14461 ttggtcaacg ctgcgctggc tgaaacaatt atctggggct tcaaggccta tcgcggattt
    14521 cagattcgtt cgtttccgtg gccctcgtcg gtggcggcta cggccggatt cggcgtcgcc
    14581 ctgttttatg gcaccaccct cttgtcgtcc gcccagcatg tcccaacccg cacgttgtcg
    14641 gtgggagtcg tacaaccgaa tgtggaacag gcacagaaat gggatgtggc atttcgccag
    14701 gagacgatgg cacgctacga tcggctcacg gcgggactgg gcgacaatct ggatctcatc
    14761 gtctggccgg aagccgccac accctttgta tttgaaatgg aaccgcagta ccgcgcagtt
    14821 gtgaacgaca tggctcgccg cgccggtgcc ccgctgctat tcggcagccc ggctttgcga
    14881 cgtcatccgg acggtcgccc gttcttgctc aacagcgcgt acctgctcgc caccgacggc
    14941 cagatcctcg gtcgctacga caagcaacat ctggtcccct tcggcgaata cattccgttt
    15001 cacaattcat tcttgttctt cctggacaaa ctcgtcgaag gcatcggcga cttcgaggcc
    15061 ggaccgggat cgacgttatt gatgttcaag ccccgcgaga agaacccgcc tgcggtgacc
    15121 gcagccaccc tcaccgattt ccacgtgaag ttcggcgtcg tcatttgtta cgaggtgatc
    15181 ttccccaacc tcgtgcggca gtttgcggcg aacggagccg atttcatggt cacggtcacg
    15241 aacgatgcct ggttcggtcc gtcgtcggcg ccctatcaac attttggcat ggtagtcttt
    15301 cgtgccgtgg aaaatcatgt cgccttcgcg cgtgcggcca acaccggcat ctccggattc
    15361 atcgacccct acgggcgtgt cgtgcaacag tcaccgatct tcacgcagga agcgctcaga
    15421 ggaacgattg ccgtcagcca tcagcagacc ttttactcgc aatacggtga tctctttgcg
    15481 tatgcctgtg ctatactcat cgcgcttttg tgcctgaccg gcttcttctc acacgacaca
    15541 gaacccgccg gtggtgaacc agccgggcga ccagcctgag cgagaaagga ccgtcccatg
    15601 ttagatgatg tccgaacccg tgtgcgtacc ctcggcgaac agttggctca actacggggg
    15661 catctttgac ctggctcgga tgaccgctga actcaacgac atcgaagtca aaacctccca
    15721 gccggatttc tggaaagata cgcaggcggc cgccaaagtg aaccgccgca aggccgccct
    15781 cgatcgggaa ctatcccgct ggcgggagac cgaacgccgg cagaacgaca tcgaggcact
    15841 cctcgaactg gcgctggaat ccggcgatac cggactggag caggaactcc tggccgaact
    15901 gggaccattc gaaaaagccg tcgcccaatt ccgcatcgag ttgctgctct caggcgaatt
    15961 ggactcgagc aacgcgattt tcgccatcca ccccggggcc ggcggaaccg agtcacagga
    16021 ctgggcgcag atgctgctgc gcatgtatgt gcgatgggcc gagcaaaagg gatttaaggt
    16081 agagacgttg gatctgctcg cgggggacga ggccggcatt aaaagcgcaa cgctgtccgt
    16141 cagcggaccc tatgcctacg gctatctcaa agccgaagcc ggcgtgcatc gccttgtgcg
    16201 gatctccccg ttcgacgcca acaaacgacg gcatacgtcc ttcgcgtcgg tgtttgtcta
    16261 ccccgaactc gacgacgatg tgcaagtggt gatcgacgac aaagacctgc gggtcgatac
    16321 gtttcgagcc ggtggcgccg gcggtcagaa tgtgaacaag gtcgaaaccg ccatccgtat
    16381 cacccacatt ccgaccggca ttgtggtgca atgtcagaac gaacggtctc aattgcaaaa
    16441 tcgcaacggc gccatgaaaa ttttgaaggc gcgcctcttt gaagtggagc agcggaagaa
    16501 ggaagccgag ttcaatgcca tcgtcggtga aaagaaagac atcgcctggg gcagccagat
    16561 ccggtcctat gtgttccaac cctaccagat ggtcaaagac catcggaccg gatacgaagt
    16621 cggccaggtc tccgctgtaa tggatggtga gctggacggt ttcatcgaag cctatctcca
    16681 aagaaaactg accggcggga aagccccgga gccggtcgcc atgaaggatg atgacctgta
    16741 aagaaaggga gctgatagcc tatggcctat agcagatagc agaaaacgga acctgttccg
    16801 gaccatcagc tattcgccag tagccatacg ctccgtgtga tcatggatga actgaacgat
    16861 caacgccaac aacgcatcaa gaaactcgac caacttcggc aactcggggt ggctccatat
    16921 ggcacgcgct ttgaggtgaa ggaccgggcc ggacaactca cgcgtctgca cggtgaaaaa
    16981 accaaggacg tgctcgagca ggagcagatc agctgcacca tcgccggccg catcgtcgga
    17041 ctacgccggt tcggtaaggc cgccttcgca gtactgcaag atggggcgga ccgcctgcag
    17101 gtgtatctga aaaaggacac attgggcgag caggctcacc agatttccga aggactggat
    17161 ctcggcgact ggatcggagt cacgggtgtc ttgttccgca ccaagaccaa cgaatttacc
    17221 gtcgaagtga ggaccctcac gtttctgagc aaagcgctac gcccgttacc ggaaaagtgg
    17281 catgggctca ccgatgtcga aacccggtat cggcagcgct acgtcgatct cattgccaat
    17341 ccccaggtac accagatatt cgcgctgcgc agccgcatcg tgtccgggat ccgcgcgttt
    17401 ctgatcgaac gcggctttct cgaagtcgaa acgccgatga tgcacccgat tcccggcggc
    17461 gccgccgcaa aacccttcgt cacccatcac aatgccctgg gcgccgagct ctatcttcgc
    17521 attgcaccgg aactctatct caagcggctg atcgtcggcg gcttcccgcg cgtgttcgag
    17581 atcaaccgga atttccggaa cgaaggcatc tcgacgattc acaatcctga attcaccatg
    17641 ttggagtttt acgtctccta cgccgactac cacgacttga tcgtgctgac cgaagaactc
    17701 ttcggccggt tggcccagga cattctcggc accaccacca tcgactatca aggcacacag
    17761 atcaatctgg gatcgccgtg gcgccgatgg tcgtatcacc aatcgattct ggaagtgaat
    17821 agcctgccga cctccgtcct cacgaatcgc gatgaggccc ttgcggcggc ccaacgcctc
    17881 ggtgtcgaag tctctcccaa agaatcactg gtgaatatcc tcaacgagat cttcgaagag
    17941 accgtcgagc cgcgcctcca acagccgacc ttcatcaccg actacccgat cgaaatttcc
    18001 ccgctcgcgc gaaggaaaga ctccgatccg tcgctcaccg accggttcga gctctacatc
    18061 gcggggcgtg aaatcgccaa cgccttctcc gagttgaacg atccgctcga tcagcgcgag
    18121 cgcttcgagg ctcaggcggc gaaacatgcg gccggagacg aggaagccca ccgcgtcgat
    18181 gaagacttcc tgcgcgccct cgaatacggc atgccgccta ctgcgggcga agggatcggc
    18241 atcgatcggc tggtcatgct cttcaccaat cagtcctcga tccgggatgt ggtcctcttc
    18301 ccgcagctga ggcctgaaaa gtagacatgg cgctgcccta cgaaatcttc atcggattgc
    18361 ggtacctgcg cgcgaagcga cgcaaccgca ccatctcctt caacaccatc gtctcgattg
    18421 ccggaattac gctcggcgtc gccgccttga tcggcacggt cgggatcatg actgggttca
    18481 aggaggacgt gcagtccaaa atcctcggca ccaccgcgca catcctggta aacgaccgta
    18541 tgaaggaatc catggtcgac tacgaagacc aggtaaaaaa ggtcgcagct gtcccggacg
    18601 tcgtcgccgc cacccccttc atcttccgcc aggttctgct cacttctccg tccggtgtcc
    18661 agggcattat tcttcgcggg atcgatcctg cgcgggaagg caccgtcacc gagttggccc
    18721 acaatctcgt caacggcaac ctcgacgacc tcgcgcgtca gaataaagtc gtcattcccg
    18781 aaaaagaacg ggagccgaac ggttccgaca cagccatgcg gccgggaatc attctgggga
    18841 aagaactctc aatgcgactc ggcgccttcc ccggcgacac cctgaatgtg gtctcccccg
    18901 tcggcccggt cacgggcgcc agcatgacgc cgaaaatccg gcaattcgtc gtcgtcggta
    18961 tctttcaatc cggcatgtac gaatacgact cctccctcgc ctacatcgaa ctgggagagg
    19021 cgcagaagtt tttcaacatg ggcgccagcg tgacgggtat cgaagtcaaa gtgaccgatg
    19081 tgttccgcgc ggcggacatt gcccgcgcga tcgagcacca tctcggattc gccttctggg
    19141 ccagagactg gatgcagatg aaccgcaatc tgttttcggc gctcaaattg gaaaagacga
    19201 tgatgttcct cctgctggtc ttgatcacca tcgtggcctc cttcaatatt gtcagtaccc
    19261 tcaccatgat cgtgacggaa aagcagcgcg agatcgcgat cctcaaagcg atgggcgcga
    19321 cgcgtaaggg catcatgcgt attttcatgc tcaatggtct gatcatcggc tgttccggtg
    19381 ccgccattgg cgtcccgctg gggtatacat ttctgtggct gatccagact ttttggacct
    19441 tcgacccgac cgtgtactac atctcccgca ttccggtcca tgtgctcggt tcagatgtgt
    19501 tgctggttgc cggttcggcg attctgatca gcttcgtcgc cacgctctat ccgtcactcc
    19561 aggcggccaa actcgatccg gcggcggcct tgcgatatga ataagagagt ccgggcagta
    19621 cgattgaacg tgaaccatta cagatcgcag caccgacatg attgacgtcg tcgatctcta
    19681 caaatcgttt ccgatgggcg ggcgggaact cgtcgtgttg aataacatca acctgcacat
    19741 caaacgcggg gaattgatcg cgatcatggg cgcctccgga gccggcaaga gcaccctgct
    19801 gcaaatcctc ggaaccctgg atcgcccgac caaaggcacc gtgtcgttcg acggccagaa
    19861 tctttttacg ctcaccgagc aagagcaggc ggaattccgc aacaaacgtg tcgggttcgt
    19921 gtttcaattc caccatctgt tgccggaatt ttccgccctc gaaaatgcct gtctcccggc
    19981 gatgattcaa aaacgcgata tggccgatat cgtcggcgaa gcgacgaaac ttctcggcga
    20041 ggtcggtctg gctgatcgac tccaccacaa accgggcgaa ctctcaggcg gcgagcaaca
    20101 acgtgtctcc gtcgcgcgcg ccttgatgca gcagcccgat ctggtgctgg ccgatgaacc
    20161 gacaggcaac cttgactctc acaccggcga cgccctcttc accttgctcc gtcaattgaa
    20221 ccgctcacgg ggaactacgt tcgtcatcgt cacccacaac gacaagctct catcgcaggc
    20281 cgatcgtatc gtctccatgc aggacgggat gatcgtgtcc tcataggccg ataccatcag
    20341 cgatggcccg cgccggctca cgcggcacgg ctgagtcgtc cgaccttgat tgggatttct
    20401 tgacggcgtc agcatctttt cgtagactga cccatcctcg cactgaagtg ggagttcgac
    20461 ctggtttatg aagacatcgt tcgcaccgta cgtccggctc acgaccctat gtctggccat
    20521 cctcctgcct cttcaggcgg cggcagtgga atttgtgttt gtcggatcgc gccaaatggg
    20581 catgggcgga gccggagtgg ccacgacatc cgactccctc gccacctatt ggaatccggc
    20641 cggcatggcg atgagcaaga aattcgatat ccgttttcag ggcagcggtc aggtcgtcga
    20701 tcgaggcgac gtgttcgatt ccctgaaaga catcaacaac ctgaatttga acgatacgtc
    20761 ggccggcaac atcgcccgcc tccagcaaca gatcgatcgc atcaaccgtc ccggcaccaa
    20821 cctcaccgca gctgcctcag gcggattgta cgtcaaaggc aacttcggcg aacatgcact
    20881 ggggttcaac gtctccgacg tcgccaccgc cggcggcttc ctgcgcggcc cggtcggatt
    20941 tacgaataac ggcaccaatc tcagcgtgaa cgggcagttc gccatgaacg ggctggaagt
    21001 ccgccaagcc gccctctcct acgcctacgc cttcatggac cggatgtttt cgatcggcgt
    21061 caccggtaag gtgatccagg gcgcggccta tacggggtct accaacatcc gtggcgccag
    21121 cgacgacgtg agagtgttcg aagacatcgg gcgcgcgaag atctcgaccg ccatcgggat
    21181 cgacgtggga gcgatgttcc ggccttcgtc ctggttacgg atggggatcg tggcgaagga
    21241 cattaacgcg ccgaccttcg atgcgccgaa cggagacaaa ttcaaattgc tcccgcaggt
    21301 ccgcaccggc gtggccgtca acccctataa ctcgttgacc ttatctgccg atgtggatat
    21361 tacgaagaac aatacgctca ccccgggcgt gtacagtcag gtcctgagcc tgggcgcgga
    21421 gcagacgatc ctgtccgaac tcctgtcttt cagggtcggc gcatttaaga atatgcagga
    21481 tgccaagaca ccgtttattc cgacggcggg cttcggactt cgcatccttg ccttgcgcat
    21541 cgatattgcc ggcgggtatg atttcagaga ccaggctgcc ctggcatccg ggtccgtggc
    21601 cttgaccttc taaggttacg ttcgcactga atcaatctgc tacacttgtt atatcaacca
    21661 tgacacgaca cacagtacgc actatcagtg ttgttttctg cctcgtgatg accggcctcc
    21721 tgaccggctg cggaggcttg caagaaatgt gggaagggcc ggccgcgcgc gggttctacc
    21781 ccaagaccat ggccattctc ccgatttccg gcacctacga tagcgcccgg gaagacgtcg
    21841 aagaagtggt ggcgaaagtg ctgatcaaga gccgtcgcgt agagaaggtc gtcccccccg
    21901 atcaggtcac cgataccttc cagaccacga aagatgcgtt cgatgcgctc gtgacctact
    21961 tctcacggat ggaaacgaca ggccaatcgg acaagaattc cgcgatcaag atcgggcacg
    22021 cgttgggtgc cgactctctg ctggtggtca aggtgaatgc ctgggaatat acgagggaag
    22081 aaggcgataa catcgcccgc gtgggcctga gcatgcggct ggtcgatgcc gtgaacggcg
    22141 ccattgtctg gaaagcgcgc caccaggtgc aggaaagtta cctcttcatc cgtccggata
    22201 tgcgggatct tgccacgaag ctggcaacgg acatgatcaa gtacatgccg ccggaaaaac
    22261 ggtagcctta gccgacccgt ttcggcccgc cctccccgac ggttcctctg cgcaaattgt
    22321 cgatcgccaa tccgaactga ttcgagagac tcgccagttc cagcacatcc cagttgctga
    22381 acggcttgcc gcttcgtcta ttcaccacct ccaacacacc cacggcctgc gttcccatgc
    22441 ggacaggcac agagacgagg gacgccaccg gcaaggcggc ttgccatccc tgaccggcaa
    22501 ccaatcgggc atcctgctct cctcgcgtga tcaataccgc ctgacctgtg cgatgcacgc
    22561 cagtcgcgat ggacgagtga agggccgaga gacccgttac gcgctcgccg gcgaccgtgc
    22621 agagtgtcaa tgctccatca ccggcactga ggaacaacgc cccggcctcc gcttccacca
    22681 agcgaaccgc ggcatccaac gcccggcgta acttctcctc ttcggccagc gacgaattca
    22741 aaagatcgct cacctgctcc atatgctgaa tccgaagaat cttcgccgcg tacttgacgg
    22801 tcagtcgcgc atggtccagc aggaccgcca gccagggctc gactgccgtc aacacagtca
    22861 gcaacttccg cgagggcaac cgctgggact tcccggtctg aaacatcacg acgccgtcca
    22921 tccggccgat ccggtgcaat cgtaaaccat acaagtcgcc gctgctcacc tgtgcattct
    22981 gttccgccct ggccaggccc tcccgccatt tcccggccac agaggcatcg ctgctcccgg
    23041 cctccgccag acaggtcatc tcgcgggtct gctcatcacg cacgtacacg gcgatcgcct
    23101 gaaccttcgt cagctgcagg aacgattgga caaatgccgc cgagacgctg atgggtgaac
    23161 ggccctcccc ttcggaaccg gacagggtat cgaacaatcc ctgttgcagg gtgcggatga
    23221 tgcgctcttc acccattgcc gcaggcacac gcgccgtagc tttgcggcgt ggagcgaccg
    23281 atgcggtttt cgatgtcgtc ttgcggttgg ttcggctgac cttacgacga gctacggcca
    23341 tggcgtctcc tcatgtgaag cgtcaaacgt tgcacggcag ggaacatctc cgctgccacg
    23401 aattccttgg atcaggacaa tacgcaacca ggagccgccg tcccggtcgg aacgaccggg
    23461 cggacaggac tccacaggaa tcatgtacga agatgttggc tgaaaagttg aacagaatac
    23521 ggagggggga gaagagctgc cggctagccc ggctgcatcg cctccttgag ggatcgcacg
    23581 aacgcaccca cttcctgaac gagtcggcca tcctgcccat gctctccaac acgacgcaca
    23641 attgcgctgc ccacgatcac gccatcggcg acctgagcaa cccgcgcggc atcctccgga
    23701 gtcgccacgc cgaatcccac agccaccggg gtcttggtgt gctttctgat cttggcgacg
    23761 ttctcgcgta cgcccgccac gtcctgtagt ttagccccag taatgcccgt gatggaaaca
    23821 taatagacaa atccgccgga ctccttcgcc acataggccc gcctcgacgc agtgctggtc
    23881 ggagccaaca gaaagatcag atggagtccg gcctcaccag ccggaccacg caacggcccc
    23941 gcctcatccg gcggcatatc cggcacgatc aatccatcta cacccgcatc cgcagcgatc
    24001 cggcagaatt ccgcctcacc gaacgcgtgg atgttgttgt aataggccat caatacgata
    24061 gggatctggg tcgaggtccg aagatgccgc accgattcca gaatccttcg aagcgacgta
    24121 ccgctttgga gaccacgctc ggctgctttc tgaatcaccg gtccatcggc gatgggatcg
    24181 gaaaagggca cccccaattc gatgacgtcg gcgccggccc gctccaattc gagcaccaac
    24241 tgctccgtct cctgcaacga cgggtccccc gccatgatgt aggtgatcag cgccttctcc
    24301 cccttcgcct tcaactgccc caatgtgtga tccaaacgat tcatatggat atacctatgc
    24361 ctctcgctcg tccttccaat caattgcagg aggttcaaaa cggtcatcca gcgcggccgc
    24421 agcaagcgcg gaggtgaggc gtactcctgc agtacgtcga acctgtgcgc gccgcgagaa
    24481 caaagctgga ggccgttctc aacctcctgc tacagcgtca cgcctttgat tctcgccacc
    24541 tgctgcacat ccttgtcgcc gcgcccggaa aggttcacga tcaggatctg gctcttcttc
    24601 attgtgggcg cgcacttcac cacttcggcg atggcatggg cgctttccag cgcgggcatg
    24661 atgccctcct cccgcgcgag cagatcgaac gcctccaacg cctcgtcatc cgtgaccgac
    24721 gtatactggg cacgattcgt ttccttgtag tagctgtgct ccggccccac ggccgcatag
    24781 tccaagcctg ccgagacgga atgggtgaga ttcacctgac cgttctcgtc ctgcagcaga
    24841 taggtcatgg tcccttgcaa cacgccgggg cgaccgcccg caaaccgggc cgcatgtttg
    24901 ccgctctcga cacccagccc cccagcttcc actcccacca tcctgacctt cttatccgga
    24961 acgaaagcgt ggaacagccc catcgcatta ctgccgccgc cgacacaggc gatcagacaa
    25021 tcgggcagac gcccttccgc ggccagaatc tgtttgcggg cttcacggcc gatgacggac
    25081 tgaaaatccc ggatcatcat gggataggga tgcgcgccga ggacggaacc cagcacatag
    25141 tgggtcgtcc gcacgttggt cgtccaatcc cgcatcgctt cactgatggc gtccttcaac
    25201 gtccggctgc cggcatccac gcctgtgacc gtcgaaccca ggagccgcat ccggaacaca
    25261 ttcagcgcct gccgctgcat gtcttccgtg cccatgtaga tttcacattc caggccgaac
    25321 atggcagcca cggtggccgt cgccacgccg tgctgacccg cgccggtctc tgcaatgagg
    25381 cgcggtttct tcatgcgccg ggcgagcagc gcctgcccga tcgcattgtt gatcttatgg
    25441 gcgccggtat ggcacaggtc ttcgcgcttg aggtaaatct tcgcgccgcc caatcgtttc
    25501 gtaaggcgct gggcaaaata cagcggcgtc ggccgcccga catattcttt caaatagtgc
    25561 ttgagttcgg cttgaaaccc cttatcccgt cgtgtgcgtt ggtacacctc ttccaactcc
    25621 agcaaggccg gcatgagagt ttcagggaca taccgtccgc cgtaagggcc gaaccgccca
    25681 tgtcgatctg gtatcgccat gtgaattcct tcagctagtc gtgaaaaatg aaagtataga
    25741 cgctgaccta gcgggagaca agcctgacgg cttgcacaaa agcccggacc ttgtcatggt
    25801 ctttcctgcc tggcgaggcc tcgactccgc tgctgacatc gacgccgtag ggccgcacct
    25861 gttcgatggc ttgcgcgaca ttccccggag tcagtcctcc ggccagcaga atacgcgcca
    25921 ccgtggccgc ttcagcagcc agcgcccaat cggccacctg tcccgtaccg ccataggcgt
    25981 cgggagaaaa ggcatccacc agaaatcccc gcacgccggc gcgcccttga aactcggcaa
    26041 gcgccagaaa tgaacatcga tcccggagac gaatcgcctt cagcaccggc cgccccagca
    26101 tctcgcaata ggccgccgtc tcatcgccat gaagctgcgc aagggcaaga ccgcaactgt
    26161 ccatgatgtc ccgcaccacc ttcgcatcct cattcacaaa cacgccgatc ggcaacacaa
    26221 acggcggcag ttccttcacg atggccttga ccgccgccgg ttccgcgcac cgagggctct
    26281 ttttatgaaa cacgaacccg accgcatccg cgccggcttc caccgccacg gccgcgtcct
    26341 cggcattcgt tagaccgcag atcttaacct ttacagccat ggcgtcagca gctctgcttg
    26401 ctcttctgca tgccgtagaa cccgcgatag gaccactggc aggcctgccc cttggcgttg
    26461 aaggtcaggc cgatcaagtc ctgccgcccg tcctgctggg gaacagacca ctcgcacact
    26521 tcgccgccgt ccttgaacga atggcacttc gtggggatgc ccatgtcctt gatgcggtcg
    26581 tctttgctgc tgcccatcca ggcatcccac ttcggggaat attcatggat ctcctgattc
    26641 atacaaccgg ccaaggtcag caacgccgcc atcacaccga gagaaagtcg cgtcttcatg
    26701 gcttctcctt gttggattca tcgcccagca actcacgaat cttcacgccg atatcctgag
    26761 cccggatcag cgactcgccg atcagcatgg catggacacc ggcctccacc aatcgcgtga
    26821 catcctcccg cttgtgaatc ccgctttcac tcacgatcag cttatcgccg ggaatgcgct
    26881 tcgccagtcg cagggtcacg cccagatccg tcgagaaggt tttgaggtca cgattgttga
    26941 taccgatcag acggacatcc gggagccatt ccaacacggt atcgagttca cgctcatgat
    27001 gcgtctccac cagcacatcg agcgacaact ctttcgccaa tgcggaaaag tcgatcaatt
    27061 ggcgcttctc caacccggcc acgatcaaca ggaccgcgtc cgcgccataa gccctggctt
    27121 catagaactg aatatccgcc accatgaact ctttgttcag cgcaggcagg ccgactttcc
    27181 gcttcacatc ggagagatac tccaggctgc cctgaaaaaa ttccttatcc gtcaatacgg
    27241 agacagccgc ggccccatgc tcccgatagg cttcggcgat ggccaccggc tcgaagcgct
    27301 gctcgaactc cggccgcaac agaccgagac tcggagaggc cttcttgacc tccgcgatga
    27361 gcgcgggcct ggtccgagtc cggcgcgcgt ccaggttcac ggcaaacccc agtgtcgggc
    27421 cggcatcgcg aattctggtt ttcagatccg cgagatagcc acggctgttc ttgtggcgaa
    27481 tttccgcttt tttatgttcg agaatccgat cgagaatcac gatgtcacga cgccttcttc
    27541 gtaaatgcga tgagctgatc cagtttctcc atcgccgcac ccgagtcgat cgtctgctgg
    27601 gcgagttgaa acccttcctg cagcgtcttc gccttgcgtc cggccacgag cgccggtgcg
    27661 gcattcagac acacaatgtc gcgcttcggt cctttccgtc cccggaaaat atcgcgcgtg
    27721 atggcggcat tgtcttccgc actcccaccg accagttctt tcggcctgac acgtgtgagg
    27781 ccgaattcgg tcggatcgat ggtataactc gacaccacgc cggctttgcc ctctgaaatc
    27841 cgggtccggt ccgtgacggt gatctcatcc aatccgtcca tgccgtgcac gacaaaacaa
    27901 tgttgcgcgc ctaggtggag cagcaccttc gccaacaact ccgtcaagcc ggcatcgaat
    27961 accccgacga cctgtatgcg ggcaccggcc ggattggtca gggggccgag aatattcagc
    28021 agcgtccgaa tccccagttc ctgccgcggc tgggcacagt gtttcatcgc actatgatag
    28081 agcggcgcga agagaaagcc gatccctacc tcgttcacac agtcggcgac ccgctcaggc
    28141 gggagatcga tcgccacacc cagcgcggcc aacacatcgg cactgcctga cttggacgag
    28201 accgaccggt tgccatgctt cgccaccgtc agcccggcgc ccgccgccac gaacgccgca
    28261 gtagtcgaaa tattgaaggt atgcgcccga tcaccgccgg tcccacaggt atctacgacc
    28321 tgagaatccc gcacccggat tttggtcgcg cgagctcgca tggcctgtac ggagccggcg
    28381 atctcggcca cggtctcccc cttcatccga agccccatga gatatccggc gatctgggcg
    28441 gaggtcgccg cgccgtccat gatctcgccc atcacggtct cagcctcttg ttcggtgagg
    28501 tccgaccgtt cggccaatgt attgattgcg tctttgatca tgaggggcgc gatcagcgat
    28561 ccgcggtcgg taatcagtcg gaaggcctca tagagagcac tgattgccgg tggccgacag
    28621 ccgtggatta gagtttcaag aaattcctga gcagctcctt gccggcggtg gtgagaatcg
    28681 actccggatg gaattgcacc ccttcgatgc ccaacatctt gtggcgcatc cccatgatct
    28741 cgccttcggc ggtctcggcg ctgatctcga aacagtccgg gagattcttg cgattcacga
    28801 tgagagagtg atagcgggtg gcctcgaacg gattcggcag attgcggaaa atggtgcgac
    28861 cgtcgtggcg aaccatggac gtcttgccgt gcatgagacg ctcggcgcgg atcacttcgc
    28921 cgccgaacgc aactgccagc gattggtgac cgagacagac gcccagcagc ggtaatttgc
    28981 cgccgaaatg ccgaatcgct tccaccgaga ttcccgcctc gttcggcgtg caggggccgg
    29041 gcgaaatgac gagccggcgc ggcttcaacg cctcgatctc ctggatggaa atcttgtcgt
    29101 ttctgtagac gaccacatct tcccccaact cgccgaaata ttgcacgagg ttgtaggtga
    29161 aggaatcgta attatcgatc atcaacaaca tatggaccac tcgtcgctcg tcgttcgtga
    29221 atcgtatctc gtggggtcgc cgttcctgcg tttcacgtct gacctttcac gtttcacgcg
    29281 ctcattccaa accctgctcg gcgagttcga tggctttcat cattgcccgc gccttgttgc
    29341 aggtttcttc gtattcatgt tcaggaatcg aatcggcgac gataccggct cccgcttgaa
    29401 tataggcctg ccgccccttg atcacgacgg tccggatgtt gatgcacatg tccatgttgc
    29461 cggaaaatcc gaaatagccc accgcgcccg catagggacc acggcgtgtc ggctccaact
    29521 cttcgatgat ttccatcgcg cgaatcttcg gcgcacccga cacggtcccc gccgggaaac
    29581 aggctcgcat gacgtcgtac acggatttcc ccttctccag ctgtcccgtc acctgcgaca
    29641 cgatatgcat gacgtgcgaa taccgctcga cctgcatcag cgactccacc ttcaccgagc
    29701 ctctggcagc cacccgtccc acgtcgttgc gtccaaggtc caccagcatc acatgttccg
    29761 ctcgctcttt ttcatctgcc aacagctgtc gcgccaggtc ctgatcttct tccggcgtct
    29821 gcccgcggcg acgcgtcccg gcaatgggac gcaacgaaat ctggccatcc tcacaccgca
    29881 ccagggtctc cggcgaggac cccaccaact ccactccggc cacccgcagg taatacatgt
    29941 agggcgaggg attgatgaca cggagggcac gatataactg gaacggagtc gtatggatct
    30001 gtgtttccca tcgttgagac aggaccgcct gcacgatatc gcccgaacgg atgtactcct
    30061 tggtccgcgt gaccatcttc tcgaaatcgg ccttgttcat gttggacgta aaggtgatcg
    30121 gcttccggcg gcgtctctgc cgcggttgcc gcaccggccg cttcaaccgc gcgatcatct
    30181 tttcgatccg cgcggtcgca tggcgatagg cgtcacggat gtcccgttcc ttggtcgatt
    30241 ccaggtaggc gttcgcgacc actttgattt tctgcgagac gttgtcgaag atcagcagcg
    30301 tgtcggtgag cagaaaggcg aagtccggca tcccgaggct gtctttacgc agcgccggca
    30361 ggtcctcgaa cgtgcgcacc atatcgtaac tgaagtagcc gaccgcgccg cccacgaacc
    30421 ggggcaggcc gggcaccgtc accggacgat attcttccat caattcgcgc aatcgctcca
    30481 gcggattgcc gcgactctga atccgcaggt tcttcttccc tcggatcaac accagatcgc
    30541 ccttctcctc ccggatcacc gccgaggagc cgcttcccaa aaacgaatac cgcgcccaat
    30601 tttcaccgcc ggccacactc tccagcagat aggccgtcgg cccatggtcg attttggcaa
    30661 acgccgacac cggcgtctca tagtccgcca gaatctcccg atagagcgga atcagattgc
    30721 cttccgccgc cagggctcga aactcatcca aggtgagaga gtattgctgt ctgctcatac
    30781 aatacgacga tcgctacact agggccggct gacaatcaac ttctgcccga ccacaaccgt
    30841 gttattgtcc aaattgttcc acttccgcaa ttgatccaca ccaacgtggt acttctgccc
    30901 gatgcgatac agggtttcac cggatcgcac cgtgtgagtg ttgccggttg ccgatgccga
    30961 agctgcaccg agggcatcca ctgccgcctg ctcgtgaccg aatgtatcct tggccgcgtc
    31021 cttcgccgac tccaggctca actccgacga cacatccgtc ttcaacgcct catcaacggc
    31081 ggccgtaaag tctttcttcg ccgccgctgc cgcctttttc gttttggatg gaatcgtcgt
    31141 caggcgcttc atatcttcat gcgcccgctt ctcaagcaaa ctggcttcct tcatcgcctg
    31201 agcctcttcc tgaatctgtg ccgcctgctg acgcaccgct tccacctgag cgccgagttc
    31261 ccgattgcgc gcctcatact cactcagttc gcgtttgctc cgtttgactt cgctgtccaa
    31321 ttcggcgctt cgcttttctt cctgcgccag gagccgctgg aagttcaggc tgcgcgcttt
    31381 ctcggcctcg tacttctccg acatcacgca tccgcccaga ttcaggccgc tcaatacgag
    31441 caacccgatc ggaactacta ccgcgcgtct catccgtgag tttcgctgac tcatcatgtc
    31501 gctccttcgt tgctgtcttc tccagggggc ttccgaatgc catcaaaaca acccgctcgt
    31561 ggcgcatccg cttccgattc gcagccttcg aaagtgggta gaatacggtg caaggcagcg
    31621 aaagtcaaag tgaaatgggc cgctcagacg tttgaccccc tccccacccc tatgctaccg
    31681 ttcgcacgag attgctgttc cgcctattct atccaatcgc cggcacgaaa gaccatgtca
    31741 cctaacacac aaccgggcac ctcccgcacc gtcaccatcg agggcatcac cctgcacctg
    31801 gcacaaccca tgaccatgga ccaggaatgg atcgggaacc gggagattct caaacagttg
    31861 ctggcctgct ggctggtgat cgacgagcgt gacctgccgc tgtctccccg catcaccggc
    31921 caaccgggga tcggcaagac cacactggcc atggctggag cccgcgaacg caagcaggac
    31981 ctctacgtat tccagtgcac ggccgacacc cggccggaag acctgctgat cacgcccgtc
    32041 ctggcagaat ccggcaccat cagttaccac gcctcgccgc tgatcactgc ggtgctcaca
    32101 ggcagcatct gcgtgctgga cgaaggcaac cgcatgaacg agaaaagctg ggcctcgctg
    32161 gctccgcttc tggatcaccg gcgctgcgtg gaatccatca tcgccggcat tctgatcaac
    32221 gcccatgccg aattccgctg ctgcgtgacc atgaacgaag acgcgtccac gtatgaggtg
    32281 cccgactata ttctctcgcg cttgcagccg acgctgggca tggggttccc tgcgcgcgaa
    32341 gacgaactgg cgatcctccg gtatcacctg cccttcgcgc ccgcagagat gctggccatg
    32401 accgtcgact ttctccagga ggcccaccag ctcagtctcg aatattccgt gcgcgacggc
    32461 attcatctcc tgcagtacgc gctgaagcgt cgcgcgcagg acccgacgca cccgctgtcg
    32521 gccgatgccg cctggcggga atcactgatg aaagtcctcg gcgaagaagc gctcgatctg
    32581 ccgactcagt cacgccggcg aaagcgcgcc ctgggcgacc aggccctgcc gcagggcctc
    32641 ggcgatttct ttttcgaaag cgacgaccct ctacatcccg gccgataatg gcagtgtcat
    32701 cctccaccgc cgactccgtc ttccgcctgt cgccacgcat cgaactgttc ccgctcctgc
    32761 acggcagcgg cgatgtcgct caggaagtgc gcgagaggct caccgaccgc cgattcgatt
    32821 gcctcgcggt tccgcttccg ccttccttcg agcatccgct ggaagaagcc gtgatggacc
    32881 tgccgacgat cagcgtgatc gtccaaccgg aacgcgatca ggaaggcgcc gccacggtga
    32941 actacgtgcc ggtcgatccc tgccaggcag tcgtcatggg gattcgcgtc gccatgggag
    33001 aaggcatccc cagggcctat atcgatcgcg aaacggccgt cgtggaaccg gtgcccttcg
    33061 tctctcccga tgcctacgca ctcaaacgcc tctcctacgc cacctttgcc gccgcgatgg
    33121 tacccacgct cacgccgcct gcggagggca gtcaacagca ggcccgcatc gcctggatgg
    33181 cgtttcaact ccaccaactc gaaatggagt acgagcagat tctctgcttg tgccacttcg
    33241 cagactggcc ctggattcgt caggcctatc agcaacgaac cgactatcaa atcccggcgc
    33301 tcgacttcgg acgccccgaa cggtacctcg tcgagccggc ctcactctat tttgtcttgg
    33361 gtgaactgcc gttcctcaca gagctgtatg agcgacgtcg cgcgaccgtc cattcggacc
    33421 gccacctctc catcgacggg atcaaagaac tcctgctcga aacacgcaca aggtggcaga
    33481 ccggccatga cgcggagggg caaaccgtcc ccacctgggt gacaccgcag ttgctgcaag
    33541 cctacttgca gtatgtgcgg aaccacgcgc tgttggatcg gcgattgacg cccgacctct
    33601 acacgctggt cctcgcggca aaacagatgg caggtgacga cttcgcggtt cgcctcctcg
    33661 aaaccgccaa aagttactcc tatcaagaga tcgaacagag ccacgtccct catctctcgg
    33721 ccgggctcgg ccggttcgcc ctgccggatg agcagatcgt gcgggcgaag aaccggctcc
    33781 agggtacgcc cctggcctgg cgttcgctct cgctccggcc ccgcccgccg cggcgcacga
    33841 gccggcagtg gtcctatctg tggaatccgc atcgacaatg ttcctggccg ccggaagatt
    33901 ccaagatcga aagtttccac acccatgtcc gcgagcaggc caaggccatc atcggggccg
    33961 atctcgtcaa gagcgagaag ttcaccacct cgatgaaaga cgggatcgac atccgggaga
    34021 gtctgcgcca ttggcatcag cggcaaaagc cgggtcgtcc gcaacggatg gagatttacg
    34081 tcaaagagat tccccccgcg cgcgggaatg tggatacggt gatctttctg ttcgacaccc
    34141 ccggcgatcc ggagcaatac agctggcagg ccacctggta tgccgaacat gcgcaggagt
    34201 ccacgctctg tttctatgcg acacccttcc tgaacaacat gatcgcaccg ggtatcgggc
    34261 aatcgcgcta cggcggcgcc ctgtttatgt tcccgccccg cccgatcccc gacatctgga
    34321 ccgacgaagc actgggattt gccaggaccc tcgaagaacg tttgattgcg gccggagcag
    34381 tccattcacg tgaaacgcac gtggccctgg tgacaccggt tccacccaag gcgcgttgga
    34441 ggcagatcgc cagacaattc gaccggcgtc tggtgccgat tccgttgagc cgcttttcag
    34501 gacagatgat cgaccgtctg cggcggtttc atgtattgaa cggacacgag atccgcagct
    34561 tcgcggctca atttattcgt gagtaaccag cccatgcctc atcagacagc cgaacaacga
    34621 atcgccgatc ttcgtcgtca aatccgccgt cacgatcacc tctactacac gaaagaccgg
    34681 ccggagattt ccgacgcgga atacgaccgg ctctttcgcg agctcgtgga cctcgaaacg
    34741 gcccatcccg acctggtcac cggcgactcg cccacccaac gagtcggcgc accaccgctg
    34801 gatgaactca tcaaggtttc ccacgagaaa ccgatgctga gtctcgattc gattaccgat
    34861 caggatgatg tccgggcctt cgatgcgcgg atgaagcggg agctggaaac cgaccagatc
    34921 gtctacacgg cggaaccgaa attcgatggc ctgtcggtgg aactggttta cgaccggggc
    34981 gccttcgttc gtggcgcgac tcgtggagac ggcatggtcg gcgaagacgt gacgatcaat
    35041 ttgcgaacga tccgcgcgtt gccgctacag ctcgatcccc agccctccct gcccgcacac
    35101 ctggtcgtgc gcggcgaagt ctacatgcgt ttggatgagt ttcaggtcct gaaccgccgc
    35161 atgacggaac ggggcgagga agcctttgcg aacccgcgca atgccgcagc aggatcgttg
    35221 cggcaactgg acagccgcat caccgcgtcg cgacccttga ccctgacctg ttacgacatc
    35281 atggcaatat ccggcacagc cccacccacc cactgggagg agttggacgc cttggccgcc
    35341 tggggccttc ctgttcccga acaccggcaa cgctgccgct cgatcgagga agtcctctcg
    35401 ttccatgccg gcaccgatgg gatgcgggac tctctgccgt ttgaaatcga cggcatcgtc
    35461 gtcaaactcg accgccggac gtggcaagac cagcttggca gcaaatcccg cagcccccgt
    35521 tgggccatcg cttacaagtt tgcaccccga aaagaaatca cggtcattca ggacatcgcc
    35581 gtctcggtcg gtcgcaccgg taccctgacg ccgctggccc tgctgaagcc ggtggaggtc
    35641 ggaggtgtca cgatcagccg ggccaccctc cacaatgcgg atgaggtcgc gcgaaaagac
    35701 gtgcgcgtgg gggacaccgt caaggtggaa cgcgccggcg acgtgatccc cgccatcgcc
    35761 gaacgggtcc ccgttcccgg ggaaacgaga caggctccgt tcgtcatgcc ggaccactgc
    35821 ccggtgtgcg gatcggctgt ggcccgcgaa ggggcttact actactgtac cggccagacc
    35881 gtgtgcgtcg cccaactgaa gggcgccatt gaacacttcg catccaagag cgcgctggat
    35941 atggacggga tgggaaagaa gacgatcgcc caactggtcg acgtaggatt ggtccgcgac
    36001 ctggccgacc tctacagcct caccaaagag cagctgctca gcctggaagg gttcgccgac
    36061 cggtcggcca ctctgctgat ggaatccata gaacgaagca aatccacctc tctcgaccgg
    36121 ctgttgatgg gactgggaat caggcaggtg gggcagcaca tcgcccgcgt cctggccaaa
    36181 cagttcggca cgttggcgaa gctgatgtcg gcgacgcagg aggaattgct gcaggtgcgc
    36241 gaaatcggcc cggagatttc taccagcctg gcctcgttct tcagcgaaga acggaaccgg
    36301 gcggtgattg cccgcttgat cgaacggggt ctgaccatcg ctccaccgtc gtctgaaatc
    36361 gcagccggca gccagagcct ggccggcaaa acgttcgtct tcaccggcgg cctggctggg
    36421 tacggccgag atcaagccaa acaattggtg gaacaacagg ggggacaggt ctcgtcgagt
    36481 gtcagcaaga agacatcgtt tgtggtggca gggaccgatc cggggtcgaa actggaccag
    36541 gcccggaaac tgggggtgcg ggttctgacg gaaacggaat ttaccgctct agtgacgcct
    36601 gagtgaccga gacatgctca tcgacgatgg gctcatgcgt gatggggtgt ttttcttcct
    36661 tgtagatatt gacgctcttg atgacccgcg ggtccgcctc atgaatgacc aggcgacaat
    36721 tggcgatatg cacttcttca ccgaccttgg gaatcctgcc cagttcatgc tgcacaagac
    36781 cgctgatcgt cacggcatcg tcgcctaaat ccaccttgag gaaatcgttg accttccgaa
    36841 cctcggtgcg tccatgcacc aggatctggt tcttgccgat acgcttgatc aactcttcgg
    36901 tgatgtccgt ctcatcgacg atctcgccga ccacctcttc cagcaggtct tccaacgtga
    36961 cgagtcccat cacacccccg aactcgttga ccacaatcgc catgtggcgt ttgtccaact
    37021 gaaactgctt catcaggtcg tccgcggatt tcgtatgcgg gatgaacagg gccggctgcg
    37081 cgatgtcgcg aagcttcatc tccgtatgcc cctgggccaa cgccgtcaac gccttggtct
    37141 tgtagagaat cccgataatg ttatccagcg tgccttcgta taggggaatg cgcgaatact
    37201 tagacttgaa caggagttcc ttcgcttcgc gcagatactg attgcagtcg agcgagaaca
    37261 tgtagatgcg cggggtcatg cagtcttcgg ccgtgatgtc cttcagctgg aagacgttct
    37321 tgatcatctt cacttcctgt gcctcgatgg cgccgctctt gctgctctga tccagcatga
    37381 tcttcagctc ttcttccgtg acgaagggca cattgagccc tttgccgccg gtcaacttat
    37441 agataagcgg cacgacgaag aacatgatcg gcgtgagcac ctgctgagcc gcataggccg
    37501 gataagccat gttcagcacc acggggaccg agtatttggc cgccagggtc ttgggcacga
    37561 gatcggcaaa caccaacaga acaaaggtca gaataccgac caggaccgcg aaggcttcgc
    37621 ccagcacacc ttccagcccc tgccctccga atcggttcag cgcaataatc gtggccagcg
    37681 atgcggtggc ggtgtcgatc agtcgatctc cgaccaggat ggtcagcagg aggcgttgcg
    37741 gatcggttcg aagatggagg gccatctccg cccgcttgct ccccgtatcg gccagcgccc
    37801 tgagcttcgt atcgttgacg gagtagaatc cgacttcaac aacagaaatc accgcggaca
    37861 acagaatgag gactatcaga acgataatgt ccataagacc tatcgaggtt agaggtgcag
    37921 actcacgttg aaggagtgaa tggtggaacc ttggatcagt ccgaagcctt ggggtcgaac
    37981 tgcaagactt gccctatgtc gtgagaagac gaccagaccg ggtcctctcg ggtcactttc
    38041 atccatcgcg tattacaatt agcacaggca gacaggcgca tcaagcatta cgacacttcc
    38101 atgccgagca caattagtca gcatatcagt catttagact atttaccatg ctcccgcacc
    38161 ctcaaggatt ccccgtagtc gcccccggcg catcgtcgcg cacgatgaat ggatcggtct
    38221 ccagtgcccg tctcagatgc gcggcggcct gctcggctgc gacctccgtt tgaaattgtc
    38281 ccgcatacac tcgataccgg cgaccatccg gaagatcgac gccgacgatc tttgaaccag
    38341 gataggcact cctcaaccgt tcgaccaaag ttcgtgcgtt ggccggatcg gcaaaggaac
    38401 cgacctgcac gcgcaacacc cccatccccc cggctcgtcc ctcgtaactg atcacccgga
    38461 gttcgatctc atcggtgccg cgcccgacca tccctacagc ctgggctccg gccaaagaca
    38521 ggtccagaat tctcccgcgc gcaaacggcc cccgatcgtt gatccgcacg gtcacctgcc
    38581 ggccggtgct cagagatcgc acgaccgcca ccgaccccag cggcaacgtc cggtgcgccg
    38641 ccgtcaattt atgcatgtcg tagacttcac cgttcgccgt gcggttgcca tggaagccgg
    38701 gcccatacca ggacgcgctg ccccgttcga taaaccccaa cggatagccg ggcggataag
    38761 aaggacgcgc cggactccag ccggagcagc cgctgagcca tgtggtgagg agaaacgcga
    38821 acagggggag aagaagacgg ctggtgcttg tcctcttctc ctccggcata aggcggatta
    38881 gaattcgtcg agtgaatggg tgcgcagcac cccgagggct ttggtcgtat tcgaaacggt
    38941 cagaaccacg atcgcgcgtt tgccttcccg cgacggggtg caatagccgc acttcacatt
    39001 gatgcgagcc ctggtgagcg aatcggccac ctgtttcaag gcgccgggct tattctccag
    39061 actcaaaatc agggcggtct cttcacggaa cttgatcttg gattttctca gcgcaatacg
    39121 agccgtatcc acatccgcca caatcaggcg aagctttcca ggaccggtga cttcgggcgc
    39181 agagaacgcc ttgatgttga ctcccgcagc cccgagcacg gccgccactt cagccagcac
    39241 tccaggctta ctctgtccac tcaccacaaa ttgcgtcgtt gtcggcatgg gctcctctcc
    39301 ctctgctgag tcagttcgtc tcggatgtta gcgactgtat ccatacagcc acttcacgct
    39361 ggtgccgccg atgtttctga cggtatggga cacaccggcc ggaatgagga tctcctcgcc
    39421 cggttcaggc cggacacacc gacccccgaa ctccagttcc aaccggccct cgaccggcat
    39481 caccaactcg tcggtcgcgt ggcggtaatc ctcccacact tgcccgggcg gatcaaccca
    39541 aagatcacaa ctgaagcccc tcgcctgcca ttccctcgcg actgccctct gatcgaccat
    39601 gaccatagtt ctccgcatct tgctacacct tagcccaacg accttccaac ttcttcatga
    39661 tctgcggcat ggtcgtgtac tccatgtcgt cgaggggcaa ccggtgcggt tcaaagggac
    39721 cgtgcatccg taacacatcg gcgatccggt tcgcatcccg gcgcgcttca tcatacgacg
    39781 gatcgtcgaa catatcccgc ggcccgacga gtgtgccgtc agccaattga aatcccaaac
    39841 aggcgacgcg cggaggccca tcgaagcggg tcggcgtcgc ctgcttcacc gagaccggca
    39901 tgagcggacc gtaatgcgaa ccacgcatcc agccctcgat gatccaggga taccggaacg
    39961 gatcgagcac ttcgcccacc gccggaaagt tctgctgtgc gcggacgatc atcacgggat
    40021 cgtctttccc gacatacttg cccgcaatga gcgaaagccg ctccgtggac gaagccgccg
    40081 caatcgtccc atccgcccgt gagtacacgt acttgatcgt gtacttaccg ggggaaccga
    40141 tgaacatcaa catgtcgtag agttcttcgg gacaatcgaa cgagatcttc ttgtgctcgt
    40201 agatatcgtg cacttcgaac cggaatccct ggtgcagcgt gggcgcgatg acgagacccg
    40261 ccgtattgaa gggatcggcg aacatcttat acagagggag attccaggca cccgccgacg
    40321 tcttgtccgc catgaaaatc agcaccggat cggacttccg ctccacgaat tccatttcag
    40381 ccacacccgg cccctgcccg cgcacgtttc cactgaaggc atcggccagc agatcctgcc
    40441 ctgcgccgta gagatgcagc tccttcgcga ccgcagtgcc ggcctcgaat gtatcccacg
    40501 caagccggtg gatcccctca tgatcgacgc cctggcgatg cgtcatgatc aactgcagat
    40561 catccccgca agccgagacg tgataatcga caagcaaccc gctggtcttc gctaccgcaa
    40621 gcttctcctt cgcgcaatcc atcaaggccg gatgcatggc cgaatgcccg acgaacccgc
    40681 cgatgtccgc cttgatcaca cttaacgtga tgctctgtgt gctctccgct gccatgttgc
    40741 acctctgggt gataaagcct ggttgatggc ctctgcgccg aatctccagc gtcggccctg
    40801 gaaggtcctc atctcggaat gagcaaaggc aatgcctgag tggctattag caaggaagag
    40861 gtgaatctga aggtgttacg ggctccgcgt gagattaccg ccggtcatac ccgtggagcg
    40921 gcgctacagc ggaagccgat cagctgtagc agaaggcagc aacgatggga tcgttcaccc
    40981 ggcaacaaca cagaggggga tgcagctgat gctgcatccc cctctgcttc aggcccaaac
    41041 aacgttggtt ggcttagaaa attatgagca accgctcgtt tctccgcagc tcatgcactt
    41101 gaggcaggtt ccgttccgta ccagcgtgaa ctgtttgcaa ttctggcagg ggtcgccttc
    41161 gtagcctttt tctttggctt cctgccgcac cgacgtgacg gtgacggtct cgcgcttcag
    41221 ttccaccttg tgcgcgacgc tgccgttgcc cggtcggtgg ctatggccgt tgccgccgtt
    41281 catgccgttg cgtcgcacgg gcagatgttc ggtgaggatc gacgacttgg cgagcgcggt
    41341 catgtcagct tcttcatcca cgcattccgg atcctgctca tccttcttca tcgaatccat
    41401 gcgcagatct tcttcacgca cctgcgacag atcggtccgc tcaagatacg tgatcgcgag
    41461 ttcgcggaag atgtagtcga tgatcgacgt cgccatcttg atgcgatcgt tgagtttcac
    41521 cggcccgttc ggctcgaagc gggtgaacac gaacgcttcg acgaactctt ccagcggcac
    41581 gccgtgctgc aggccaagag aaatggcgat ggcgaagttg ttcatcaagc tacggaaggc
    41641 ggcgccttcc ttgtgcatat ccaggaagat ttcgccgagg gtcccgtcct catattcgcc
    41701 ggtacgaaga tagagcttgt gaccgccgat gatggccttc tgcgtgtacc cgctgcgtcg
    41761 cgccgggagc gaccgccgct tgtggaggta gcgaaccagt acgcgctcgg ccactttctc
    41821 agccacggtc ataacgctgg gcgtcgcttc cgcgcccttg ccgctgtcgg acgaggcgtt
    41881 cagcggttgg ctcagcttcg acccgtcgcg atacagcgcc acagccttga ccatgctctt
    41941 ccacgcaaac agatacgccg ccttcacatc gtccaaggtg gcatcggccg gcatgttgat
    42001 cgtcttgctg atcgcgccgc tgatgaacgg ttgcgccgcc gccatcatgc ggatatgcgc
    42061 atcaacggca atatatcgct ggccgatccg gccgcaacga ttggcgcaat cgaagatcgg
    42121 caggtgctcg agcttgaggt ggggcgcgcc ctcgaccgtc atggtgccgc agcaataatc
    42181 gttcgcggcc gccacatctt cctgggtaaa gcccaggtgc ttgagcatgt tgaaggtcgg
    42241 gtccgccagc tgcgcttcgc taatgccgag tttctgccgg cagaagtctt ctcccaacgt
    42301 gtacttatta aaaacgaact ggatttcgaa cgcctgcgcc aacgaccctt ccagccgctc
    42361 cagcgccgca tcgtcgaagc ccttttgacg cagcacttcg tgattgatga agggtgcgcc
    42421 tttcaaggtt tgatgtcccg cgcaataggc cccgatgtcc ctgatctggg catcggtgta
    42481 cccgagcgcc tgcaaggccg gcggcagact ctggttgatg atcttgaagt acccgccgcc
    42541 cgccagtttc ttgaacttga ccaaggcgaa atccggttcg atgccggtcg tgtcgcaatc
    42601 catgaccagt ccgatcgtgc cggtcggagc gatgacggtc acctgcgcgt tgcggaatcc
    42661 ataggccgcg cccaattcca aggcgcgatc ccaggcgcgt cgcgccgcca gcaacatggc
    42721 cggcggacaa tgctccggct gaatcgaggt cgggagaatc gtgagacctt catactcttc
    42781 atgcggtgcg ctgtatgccg cgcgccggtg gttccggatg acgcgcaaca tcgattcacg
    42841 gttcttgttg tagctcggga acgggccgat ctccgccgcc atttccgccg acgtcgcgta
    42901 cgactcgccg gtcatgatcg ccgtgatcgc accgcaaatc gccgtcgcct tcggtgaatc
    42961 gtagggaatg ctttgtttca tgaggatggt gccgaggttg gcatacccca atcccagcgt
    43021 tcggtactca taactcttct gcgcgatcgc acgactgggg aacgaggcca tcaacacact
    43081 gatttccaac gccacggtcc acagacgcac cgcgtggcgg aaggaatcca ggtcgaactc
    43141 cccttccaca ttaaaaaact tggacaggtt caacgaagcc aggttgcacg ccgtgtcgtc
    43201 caggaacatg tattcggaac agggattgga ggcgttgatc cgcccgtctt cggggcaggt
    43261 gtgccactcg ttgatcgtcg tatcgtattg ggtcccggga tcggcgcaga tccaggccgc
    43321 ccaggcgatc ttgtcccaga ggtcccgggc cttgaccgtc ttgctgatct tgccgtcgat
    43381 ccggcggcgc aattcccagt caccatcccg ctccaatgcg tcgaagaacc cgttcggaat
    43441 acgcacgctg ttgttggagt tttgccccga gaccgtctgg taggccttgc cgtcccagtt
    43501 ggtgtcgtac tcgtgaaaga cgaaatgtgt gtagccctgc ttcgcgtagt cgaacatgcg
    43561 atggatatag gcctccggca ccatatcgcg ccgggccgcc gtcacggcat cacgcaacac
    43621 ctggttcttc ttcatatcca gttcgacttg gcccaatccc tgcgcatcca ccaacaggca
    43681 ggccttgagc acggcgttca gccgttgcgc gcaaatcttg gagccggtga ccatggcggc
    43741 caccttctgc tcttcgacga ctttccagtc gatgaactct tcgatatccg ggtggtcgag
    43801 gtccaggcag accatcttgg ctgcgcgcct ggtcgtcccg cctgacttga tcgccccggc
    43861 cgcacgatcg ccgatcttca ggaacgacat gaggccggac gagcggccgc cgcccgacaa
    43921 ggattcaccg tctccgcgca accgcgagaa gttggtgccg gtgccggacc cgtatttgaa
    43981 gagccgcgcc tcacggaccc agaggtccat gatgccgttc tcgttcacca gatcgtcttc
    44041 aatcgattgg ataaagcagg cgtgcggctg cggatgctcg aaggcattcg tcgcccggac
    44101 gacctcgccg gacttcggat cgacatagaa gtggccctgc gccggtcccg aaagcccata
    44161 ggcatagtgc aacccggtat taaaccattg cgggctgttg ggagcggcca tttgaaaggc
    44221 cagcatgtag cacatctcgt cgtagaacgc ggtcgcatct tccggcgtct tgaagtagcc
    44281 gtggttcttg ccccagaaag tccagcaacc ggccagccgg ttgaacacct ggcgggagtc
    44341 gcgctccccg ccgagcacgg gcttgcctgc ggcgtccacc accggcgtgc cgtcctcatg
    44401 gacttgcggg actccggcct tccggaaata tttttgcgcc agaatatcga tcgccagttg
    44461 cgtccaatgc tccgggatgt cgatatggtc caatttaaac acggtcgatc cgtcgggatt
    44521 gcggatctcc gaggaacgtt ttacgaacgc aagaccctca taggggctct gcccacgctt
    44581 ggtgaatctt cgttcaatcc tcactggttc ctccttcggc agatgcgaga cgttcggtgc
    44641 agagaacggt cggcgatgat gtgtcggggt gtgattggaa aagacggttt ctcaacagag
    44701 tcaatctcat ccataaccgc gttacctact agatatagtg atcacatttt tttgtcaaac
    44761 aaaatgttgt ggcacacctg tgattaatgg ggataagctg gtgatagcgc cgacgccaga
    44821 tttgatgctg gttttattca tttttcacag agttatccac agcgatcctg aggctgtgga
    44881 aggaggtttt tcacagggag gcgagaaaat ttctggcaca ggctaaaaag ccagccgacc
    44941 gacctttaac gtcgcctcct cgacccctcg caagggcaca aattgaccgg ccacacccag
    45001 caccaatacc ggttggtcca gcacattggt ttcgaagaaa ctgagcaggc cccagttgtt
    45061 cctcgtggtg ttcggccgaa cgacgccctc caccagtttg gggccctgcg cgcgaaagac
    45121 ggctttaatg acgtcacgat cgtggcgcgg cccggactgg tccatctttt cgatttcggc
    45181 caacaggcgg gcttcgacga aatgcacata ggcatccatc gtgggattgg tcgccgccaa
    45241 tcccacggca accagcagac tccccaccaa cccggccaac gtcacattac tcatcgtctc
    45301 ccttcgtcga aggcagtacg cacaggcttc atgtccgtcg agaccacaat ctggcggttt
    45361 gacagattgg accccgtccg tttagcttac gcccgatttc tgaggaatgg aaccctcacc
    45421 cggacttgag agcagccccg gcaggcttcg gtctcaacgc ctcgcggcgg cattcatgaa
    45481 tgatccgggc cggctgattc gatcacactg gttcgacggt aacggaagga gcgcctatgt
    45541 tcgtgtggag taaacgcctg gtgacagccc tcctggcagg gttcgtcctc cttatcggag
    45601 tcttcctcat tcagggagag ccggaatcgg ccagcggatt gaagattcac accacgcgct
    45661 ggatggccgt gaattacatc ggcctgctcg tgtggatctt tatctggatg ttcgatcaag
    45721 cccgcgtgcg agggaaaaac gtgctgctct ggctgctgcc cttcgcgctg gcaccgctcc
    45781 cgaccctgat gctgttcatt ttgtttttac agaggaaggt gaagtaggag gcgatgctga
    45841 aacagcctgg cggatcaaga cgaggatagc ggcacgctca ctgccgaggc ggtggcgtcc
    45901 tcgatcctga ggtcagccgg cacaggcatc aggccatgct gagttacgag gcccaccgca
    45961 aatcaagttt cttctgactg agcgcacaat ctcgcaaata caaattgatg aggttctgat
    46021 acggcatccc gatttcgtcg gccaatccct tgaaataggc tacagtcgcc ttatccaacc
    46081 gaatcgtgat gggctgcttc agttgtccgc gatacggatt gcgctgcccc ttcatctttg
    46141 agaaatcata ctccgccctc ataggtcacc tctcataatg ctcgccctcc tgtcttgtcg
    46201 cttttctggc cgaaataatc cggatgaggc catcacgttc tcgatagcag tggcagacaa
    46261 ccagaatgcg gagcgtgaaa ctcaggccca gcatgagaaa acgatcctcg tcctccgaat
    46321 gatcaggatc gaaaaatcgc aacgcatggt cgtccagaaa taccgactgc gcctcctcaa
    46381 acgagacacc gtgctttcga atattgtgtc gatttttctt ctcatcccac tcaaaccgaa
    46441 taccagccat gcgtacagtg tacatatatc cggtgcatgg ttcaatccgt gactaacagt
    46501 ctgtccgcca acaactcagg aacagaagca ttcgggaaga gccggaatcg gccggcggct
    46561 tgaagattca cactacgcgc tggatggccg tgaactacat cggcctgctg gtgtgggtct
    46621 ttatctggat gttcgatcaa gcgcgcgtgc gggggaaaaa cgtgttgctc tggttgcggc
    46681 cgttcgcgct ggcgccgctc ccgaccctga tgctgttcat tttgttttta cagaggaagg
    46741 tgaaacagga gggagcactg taataaccag ccgattccta tgctgatcgc aatatccagc
    46801 tgtcagcagg gaacctcaac tccctctctc cccgcattaa gttccgcatg ttgcgtcacc
    46861 ggcttgcgga gcacccacag cagccaaagg ccgggcagcg cggcgagaaa ggtcacgaag
    46921 aagaagggtc cccacccggc gagccctacc agcttcgcag cgggccagcc gagaaaaacc
    46981 cgtccgagag cttcaacgga agagagcagg gcaaactgag ttgctgtgta gcgatgattg
    47041 cagagcgaca taaccagcgc cacgaaagcc gccgtgccca tgccgccggt gagattttcg
    47101 aatccgactg cgaacgccat gacggcgtaa ctcttcccca cccaggccag ccataagaac
    47161 gacaggttcg acagcgcctg taacaccccg aagaccatca gcgaacgaaa gaggcctaac
    47221 tgcggcatca tgccgccgcc gatgaacgcg cccagaatcg tcgccccgat gccgaaggcc
    47281 cggaccaggc cgatatcttc cgacgaaaat gccatcccgc gcagcaggaa cgaagtcgtc
    47341 aatgcgccgg cgaaggcatc acccagcttg tagagcacga tcaatcccag cagcgccagc
    47401 gccatggggc gagacaaaaa ttcagacagg ggcccccaga ccgcctccga catcgactgc
    47461 ggggccgccc tcgccgcctc cggttccggg atcattaaga tcgtgacaat tccgagcgcc
    47521 atcaacccgg ccatgcagag ataggccgag gaccatccca ggcgcgccgc aacgatcaag
    47581 gccagcgaac cggacgccat catcgcgatg cgatacccca tcacccaggt ggccgcaccc
    47641 agcccccgct catccggttt caacaagtca gtgcgatagg catcgaagac gatatcttgt
    47701 gaagcagaga gaaaggcgac acccaatgcc aatgcggcaa agatctgggc gccggagcct
    47761 ggaccgatga aggccattag cgtcagtgcc agtgcaagac ccgtctgcgt gacgatcatc
    47821 caaccacggc ggtgcccaag ccagggcggc acgatgcgat ccatcaccgg ggcccagaga
    47881 aatttcaacg tatagggaag gccgacgtag gcgaacaggc cgatcgtggt cagatccagg
    47941 ccggcctcgg tgagccaagc ttgcagcgtt ccacccgtga gggccagagg caacccggag
    48001 gcaaacccca ggggcagcat gatgccgaga cgtggagtga gaacggcgcg cagggtcgct
    48061 ctgttcatga gattgaagga gaccgggaga tcacgaggtg cagcatacca ccgccacgcc
    48121 tctcctgcgc atcagaatcg tgaagtccac ttctaccact tcctgacgaa gcctgacgaa
    48181 accgactccg gtacaccgtg gccgaacgtg tcacatgcgg ctcaagtaca gagaaagccc
    48241 gctctggatg gaaccaaaaa ttttgatcaa aagaccgctt tcatacggtc ccaacgactg
    48301 gcctggttgc aacttctact actaacgcgc ttaaagtgtt actctctttg acggtcgatt
    48361 tcccagtaca aacagtctca tttacgctga ttcggtacca tgaacttaag gaggtctcgc
    48421 catgctcagt gatctggagc acatccagct tgctgaagag aaggtccgcc gctttattac
    48481 cttggctgag gaaattctaa ggcttcacga caaagccgaa caacgtatta atgaagggta
    48541 ccgcaacgaa tatttcaaac ctaccctgag aaatcgtgta attgcaggat tagcggtcaa
    48601 agcaggacgc tcgtttgaac gtctcatcgt tgatgcccgt aacagacgag gagaatgtgc
    48661 ccatcatttg aagacaatgg ttgagtgctt catctatgcg tgttgggtaa gcggcgatga
    48721 gaccgaaaaa agagcaagcc tgttacaagc tgagagcttt cgctcacgag ctgcatacca
    48781 tgaaaactgt tctgcttctc cagaagacga agatgcgcat tgggctaaag agtggcgtga
    48841 gcttcagcat aagcagataa agggaattga aaaggaatgg aaaaatttcc cctctttgga
    48901 aagtatcgca gctgatgtga agagcaaaga cctttacaat atctatagag cgtcctgtga
    48961 agcagcccac acgggagacc ttccgctcta catgccgcca caaccaaccg aacccggcct
    49021 gaccctagcg gatttgtcct tgcagcagtg ttacgtctct ctccaatcgg gagtttttct
    49081 cgcctgcgag ttactccgta acgcttctga tcaagtggaa ttggagctcg agtcggaaat
    49141 tgatagtttt gcccagagaa gccgagagat tcgactactg gggacttagc cagaggggaa
    49201 cagcaaagaa ttgacagggt cgataccgtt tgagcatctt acacgcctac atgagtcgct
    49261 catgggttag atcgcccggc cggctctgtc cggaggtata cgaaccaata gaccagcgcg
    49321 accagcaccg tcccgccgat gatgttgcca agggtcacca acaataggtt agtcacggca
    49381 tccatgacgg acaagggcgc gaccccaccg gcggccagcg cgatgcccag cggcagaaaa
    49441 aacatgttgg ccacggaatg ttcaaagcca caggccacaa aggccgtgat cgggaacaca
    49501 atggcgagga ttttatccgc cacgctccgc gctcccatgc ataaccaaac cgcgagacag
    49561 accagcacgt tgcacaggat cccgcgggca aaggccgaaa ccggatccag cgcgatcttg
    49621 ctgcgcgcga tctgaaccat ggtttccccg acggccccgt ctgccaatgt ctgcacgccc
    49681 gcgagcagga ccagcaccgc cgtcccgccg gcgccgagca ggttccccag atacacccat
    49741 acccaattcc tcaccacctg ccgcgtctgc acacaaccga cggcccaggc catagcgata
    49801 aggttattcc cggtgaagag ttccgcacct cccacgacca ccagcacgag tcccagactg
    49861 aatgtcaccc ctcccgccac acgcaacagt ccgaacggca gcgccggacc gcttttcccg
    49921 gacgtgatcg tcacagtgta gaaaagcgca cccaacgaaa taaacgcgcc tgccagcacg
    49981 gctaacgcga tcatggtcgg cacaggggtg gtgaccttgg cgagccccac cttgcagacc
    50041 cgtgccgcaa tctccggtgg cggataggcg tcggcaacag gattggtagc ggcaggagtg
    50101 ggttctacgg tcatgtgatc aacatcacac gcatgggggt agaaggagtt ccagccggca
    50161 ggattttctc aattcgcacc gtcgagactt tatactccgg gcatttcgaa ctctcatcgg
    50221 cgctggagga gagcaacacg ttcagatccg tgtcggggaa gtgaaaactc gtgaacagtt
    50281 cccccggctg cacccgctca ctgaccatca caggaagctt ggcttcgccg cgcgcgctca
    50341 ccaaccgcac gatctcgccc tcgtgcagat ccagccgggc tgcatcggat gcgtgtattt
    50401 ccaacacatc agtatcgacc acctgcatga tctccgtccg tctggtctgc gcgccgcaat
    50461 tgtaatgctg gaggatacgc cccgtgatga gtaccagcgg ataggtctcc gtcggcgact
    50521 cccctggcgg cagataatcg accgccacaa actgcgcctt ccctttcgga aacgatcgct
    50581 cgtgcatcaa cgaggtgccg ggatgctcct tcgacggcac cggccactgc aacccctccg
    50641 gcgcctccaa gcgatcatac gacacgccgg caaacatcgg cgccagctgc gcaatctcat
    50701 ccatgatggc ggaggggtgt ccgtaccaca tgggataccc catccgcgcg gagacttcac
    50761 agaccacctg ccaatcgggc aacacgccgt ccggtggttc caccgctttt ctgatgcgtt
    50821 ggatacgccg ctccagattg gtgaacgtcc cgtctttctc caggaaggag gcacccggga
    50881 tcaccagatg cgccagcttc gccgtctcgc tgagaaacag atcctgcacg atgaggaact
    50941 ccagattctt gagcgccgca tgcacctttt tcagattggg gtccgtctgg gccacgtcat
    51001 acccgatgat ccaaaggctc ttcagtgtgc cggctaacgc cgcatcccac atgtccggcg
    51061 tcttcatccc acgcgtcgtc ggcaagggtc gccccgtgat agccaaatgc gcagccgcca
    51121 gttcggggct gtcgaacggt tgatagccgg cgaaatacgt cggcaggcag ccgacatccg
    51181 atgcgccctg tacattattc tggccgcgta acgggttgat gccggtgccc ggcttgccga
    51241 ggttgccggt ggccaacgcc agatttacca gcgccatcac cccgtggcta ccccagcgat
    51301 gttcggtcat acccaggccg tgcacggcca tcgagccgcc gcttgtggca taacgacgcg
    51361 ccgcttcggc aatcagatga gccgggacgc cggtcacctt cgacgtggtc tccggcgtgc
    51421 aggactccac taccttcacc cattcttcaa agccctgcgt acggtcgccc acaaaatcct
    51481 gatcgatcaa cccttccttt accaggatgt gcccgagtcc gttcaatagg gcgacattcg
    51541 tgccggggtg catttgcaga tggagatccg cgaggcgggc gagttcggtg cgccgtgggt
    51601 cgatcacgat cagcgcggca ccccgccgta cggcctgttt gatacgcgcg ccgaccaccg
    51661 gatgcgattc cgtgggattg gccccgacca ccaggatcaa cttcgccaga tccagatcct
    51721 gaatggaatt ggtcgccgcc gacgcgccga tcgtgtccat catgccggtc acggtggcgc
    51781 tgtggcagac ccgcgcgcag ttgtcgatat ggttgctgcc gaccacgcag cgcatgaatt
    51841 tcccgaacag ataattttct tcattcgtgc cgcggctcga tgaaatcgtc gccagcgcat
    51901 cagacccgta gatgtctttc agacgtaccc actcgtgtgc gatccggtcc aacgcgtcgg
    51961 gccaggagat ctccacccac ttatcgccct gccggagcaa cggcacgcgc aatcggtccg
    52021 gcgcatagtt gtacgtccaa ccgaatcgcc ccttcatgca ggcgtgaccc tgattggacg
    52081 ggccgtcatc ggcaggcacc atgctcacga ctgtgctgtc gcgcactccg gcgttgaagg
    52141 cacagccgac accgcaatag gcgcaggtgg tccgcacagt ccgttcgggc gtcccctgct
    52201 cagcgacagt tttttccatc agggccccgg tgggacattc tttcacgcag gcgccgcagg
    52261 agacgcagtt cgaggaagca aacccggccg cctccccggt taacgacgct gcccccgcga
    52321 ccggccggct cgcaaagccg cgatggagca tcgtcaacgc atgggtccct tgaatttcgt
    52381 cacaggcgcg cacacagcgg gcgcaggaaa tacaggctgc attgtcgaag gtgaaaaagg
    52441 gattggatcg atcgacgaca tccagccggt gggagggctg cggataccgc acctgtcgga
    52501 gcccgagcga cttggccaac cattgcaggg attccggagc ccgatctccc gccggttgtt
    52561 cggacaaata gagttcgacg atgttccgcc tgagccgagt cagccgctcg ctctccgtgt
    52621 ggaccaccat gccctcgcgc accggagtcg tgcaggacgc aggcaagcca ctctggccct
    52681 caacctcgca gaggcagagc cggcaggagc cgaacggagc caaatggtcc gacgagcaga
    52741 gcccgggaat ggcaatcccg gcctttttcg ccgcgccata aatcgtgtcg cccggcgacg
    52801 ccagcaccag acgcccatta atttccagct tcacgcgggt ggctccgtat ctcgtcgttc
    52861 gtctctcgta gctcgtcaac agcgcttcag gagatgcgtt tcatctttcg catttgacgt
    52921 ttcacgcctg acgtttcacg tagcccgttt cacgtcctca acttgcttcg aaactcggca
    52981 aagaaatgtt cgattgccgt cagcaccgga tagggcgcgc gcccgccgag ggcgcagaga
    53041 ctggcgacct tcatcgtgtc gcccaaatcg tggagcagcg ataggtcctc gatggtgccg
    53101 ctcccggcct gaatccgttc gagaatttca cggcctcgca ccgatccaat gcggcagggc
    53161 gtacatttcc cgcaggattc gtcggcggta aacgccataa aatgccgcgc cagatccacc
    53221 atgtcggtct cgtggtcgta gacgacgatg cctccgtgtc ctaacaccgc accggccttc
    53281 gcgaatgcgt cgtaacaaat cggaatgtcc atctgggagg ccggaaagag acttccgagc
    53341 ggtcccccga cctgcacggc cttgaagcgc gatccgggcg ccatgcctcc gccgaattgc
    53401 tccagaacct gatgcagact caatccgaac ggcacctcga ccaacccggg atgtttcacc
    53461 ctcccgccga gctgtaatgc catggtgccg cgcgactgct ccgtgccgag cgccgcatgc
    53521 caggcgccgc cgcgcgaaag aatgttcggg atggtcgcaa aggtcagcac gttgctgacg
    53581 atcgtcggtc gtccatacag gccggactgt gccggatagg gaggcctcgc ccgtaccacg
    53641 cccctcttcc cttccagcga ttccaggagc gccgtttcct ctccacagac atacgaaccg
    53701 gcaccttcca ccacttcgat tggaaacggt tcgccgtcca actccaacaa ctccgcttca
    53761 tccgccttct gaatggctgc ccgcaacgtc ttcgcagccg ccggatactc ctgccggcag
    53821 tacacgtatc cgcggccggc cccgatggct ctcgcgcaga tcaacatccc ttccagcaac
    53881 cggaacggat cgccttccag aatcatccgg tcgcagtagg ttcccgcgtc gccttcgtcg
    53941 gcgttggcga ccacgtactt ttggtcggcc cgcgcctgtt gcgccacctt ccacttgttc
    54001 cagaccggga atgccgcgcc gccacggcca cgcaactgcg acacctgcag ttcttcgatg
    54061 atcgcatccg ggccgatccg taaggccgtc tccaggccgg acagaccgcc gcgcgcctga
    54121 tattcatcga gcgccaacgg ctccgtctcg ccgaagttgg caaacgtgaa cctggtttgc
    54181 tgagcaagaa agggaatgga tcgcaccgga gtgccgtctg tgccggatag aatgagcggt
    54241 aaatcctggg ctgtgacatt gaaccaggcg acgcggccgg cggggctatc gcgctccacc
    54301 atcggttcaa gatagaacgc gccgcgcgag gagatgcgaa tgagttggac ctcgggatgt
    54361 tccgaccagg catcggccag cacaccggcc cccgcggccc gtgccgacgt atcgttggac
    54421 aggtagagtc tggtgaccgt acgcatgcta cttgtaccgc tccagcaacg tatcgagctg
    54481 cgagggcagc aggcggccat agaggtcacg gtcgatcatc accgtgggag acaccgcgca
    54541 gttgccggca caagacatcg tatcgagggt aaattttccg cccgcggcgg tttcgcccac
    54601 gtccacacgg agacgatcct gtaattcccg cagtagccgt ccgcagccgt tggcgtagca
    54661 cgattccccc atgcagacac ggatcagatg ccggcccgga tgcgtcaggc gtaaatcggg
    54721 ataataggaa agcacgccgg cgacttgcgc ggtcgtgacg cccaacgcat gggcaatctc
    54781 cgacaccgcc tctgccggca catacccgag ttgctcctgc agagccagca gggctttgag
    54841 aatattggac ggctcaccgc gaaccgtctc gagaatggac ttcacctgct cgtccatggg
    54901 tgcgctcggt caaggatttc gtcgctcgtc gtccgtctct cgtatctcga tgcagaacag
    54961 gagtacatag cgtattgctt atgggtatca ttagaagcga cggcaacccg cggcgttctg
    55021 ccttcatcca tgagccatac gctcttcttt cctgccatca gctatgcgct cggagcctgc
    55081 tgagatacgc ttcacgaatc gacgtcagca gagccccacg tgcgcgcgga gtttgttgta
    55141 atcgaaggcc gcaaccccgg tgcgaaggag gccgaaatcg ttccgttcca tctcatcgat
    55201 cagcgccgcc acctgagtgc gcccggactg ggtcggcatc acatgccgag gggtctcacc
    55261 gccgactgcg ggagactccc ggtcggccca ctcgaggtac acggtttcca ggaaggcatt
    55321 catcaacgtg cgatcttctt catccgtgac caccaccgtc aacggctctg cggcggacaa
    55381 ctccgcctgc gtcacctgcc gcatcggcgg ttgaactgct tcaccacgga agtgcagtga
    55441 aaacccgaag gcagccgcca aactatgttt gaccgtatcg agccgctccc gcgagtcgca
    55501 ttcgagaaac agttccgtcg ccgtcacggt caccctcgct gtcgggcttg ctcccgcctc
    55561 tgccccacta ctcccctgaa tgaagcgccg gagctttccg gaagccgtcg caccggcgga
    55621 acgaaccgga gcctcctctt tccacccctc cagctcagcc atacctccgg cgataaacgt
    55681 gaactcacga tgttcataca atgccatgac atagaaataa ggggcgccgg cagtcgtccg
    55741 ataacgaatt tcgctcaccg cctcgaccag ggccgccagg cgcatgcgtg caaaattccg
    55801 caagagcaga tgcccgaacc gtttggcgaa ttccggccag tcgcccagct cgaatgagcc
    55861 gccgacgacc tccatctcgc gacgctcttc accggtcgtt tcgtagagtg ccttggcgtc
    55921 ctccgccgac aacatcaaga ccggcccagc cggcacagcc agcgggcttt caggatcacc
    55981 cggctcccga acgagcctcg tgaacaacac ctgtccgctt tctgcgtccg atcccaggct
    56041 gtcggccggc acctcgtaca cgcgcccgtt tccgatcgac cgtagattcc gccgttcctt
    56101 cccgatctcc tgtggcagca attcgacgag atccatatag gaatgtttga gcggatccag
    56161 ccagacccgc tcttcctcgg gaatatgttc agtgatgacg tcacgcagct gctcgatcag
    56221 ggtcagctgg ccgtcttccg ggtagaagtc tgcatagagc aacaaatcgg ccagcccgac
    56281 ttcttccggc aacggcgaca gaggggcctg actacccaat tcaacgtaca cttgcaacgc
    56341 gcgcgtgaag gcaatccggc gctgttgggc aaaacccggc gtcatcgaca gccgatcgag
    56401 ccgcatcagt atgatgtcca gctccttcgt caccggcacc gatcgcccga cgactcgttc
    56461 attcgcgagc ttcatgaatc tcctccatga cggccgtttc cgcatccagc caatcctgaa
    56521 gcgcgtgtcc ctcccgacac cctcgctccc tccacaactc aaaggccttc tgagagattc
    56581 gttcccacat gccttccggc agctcgattg gattcgtcac ggccgagctg acggctcgaa
    56641 ccttcgattg cggagcggac ggctttttca ctttgggctt catgggggac tcctttacga
    56701 cagcctgctg gttatgcttc cccgagaggc acgacacgtt gatagttcgc gccgaaattg
    56761 aactgacgca ccgtgagcac aggacagggc gcgtggcgca gcatcgcgcc ggcaatgctg
    56821 ccgacgagga cgtgggagat gccacgccgt ccatgcgtcc ccatgatcat caaatcgtac
    56881 ccttgctggg tgacatagga tgcgatcgaa tccgcgggca gtcccggttt caagacatga
    56941 tcgacttgaa gaccctgcgc ctgcacacag gcgaccagca cggtgaggcg tttctccaga
    57001 taagcccgct gctccttcca ctccttggca tgactcagac tgaagtccaa tccatacgcc
    57061 accggctcca tggcatgcag aatggtgatc gacgctccga acggtttggc aaactggacg
    57121 gcatattcca acgcatcgag cgaacaggcc gagagatcga tgggcaccac gattcgtttg
    57181 atattcgcca tcgcggggac agccgcactg cccttgtcgg ccttcaccgc caggaccgga
    57241 caaggcgcca tacggacgac ccgctccgcc gtgctgccga tcagaacatg atctaatccg
    57301 gtgcgcccat gtgtaccgac caccagcagg tccgcgccga tagtgtgggc gaccgtctgg
    57361 accgcctggc ttggaatgcc ggtttcaatg cgcaccgtca ccggctgtcc ggccgccttg
    57421 gcgcgtgcct ccactgccgc caactgacgg ctggcctcgg cgcgcaagtg atccaggtac
    57481 atcttgttca cggtatagtc cggatccatc cccgggtaga gctccaggac cgtcatcaca
    57541 cagagctccg ccttccaggc cgacgccatc gctaaggcat aacccatggc ccggtccgca
    57601 cacgccgaaa aatccgtcgc aaagagcact cgcttgatca gggtcatgac ttagaccgcc
    57661 tccacgttta acaggagcag ttcgcctttc atgttcggat gaatggaaca gcggaactca
    57721 tgccgacccg gccgttccat ggtaaagcga acgacggctt ccttcttcgc atccaaaaac
    57781 aacccgccga tctgcttgcc atacgacacg atcccgccgg actcgacgcg cgtcggaatg
    57841 ccgtcgaaca tggtcgaccc gaaatcgtgg cgcacttgat cctgattgac gatggcaatg
    57901 accgtcgcca cccccagccg gagcggcacc tgtttggtga caaagacgga gtccttgatc
    57961 gtcacttcga tacgctgctc cgcctgcgct ccaccctgcg aacagagggc cagaacgaaa
    58021 caagccgcca accatacccg tcctccgctc aacaagtccg acaccttcct cacacccata
    58081 agtgacctcc tttataaact cttgcccgtt ttcactttcg cccgtttcgt ggggagcggc
    58141 actgtcaata caggacaccc ggccgtccgg acaaccttct ccgccgtgct gccgaagaaa
    58201 atcttatcga ccccgccgat cgcacccccg taacttccca tgaccaccaa atcgaccccc
    58261 tccacccgag ccgtgcgcgc aatttcctcg aaggggcagc cgtccgcgag cagccggcgg
    58321 atcgtcaccc ccttcgcgtc atccctggcc aacagttgcc gggcattgag acgggcgaaa
    58381 tgatgcagct gctttttctg tttcgcggct tcagtggcag gcgccagccc gagtcgattc
    58441 agcgcgtcta aacttttggt atcgacgaca tgcagcagga gcacctccgc ctgatacgat
    58501 tttgcgaacg acagcgcaag ccggaaggct tcttcggagc agggagaaaa atccaccggc
    58561 acgaggattt tcgtgaacag acgatcggcc attacacctc cactcatcac tcctacctct
    58621 agcaccagta ttagcaagat cagcaaggcc gatgccatgc agaaaggagc gaggggcata
    58681 gagcctctgg cttatggaac agaggatttt gcagacatca gggccattgg ccatacgccc
    58741 taggctcttg aacttcatct ccggggggtg tcctgctaca gccaatacca gtgttctgta
    58801 gcggaacgcc acagagcaac aaagagctta tggcttgtag caaatagcac atgctgacgg
    58861 agatttctcc tatcgtgcca tcagctacag gcgcttagac ctccctttgc cgtccacccc
    58921 tgtgccgatg ctcggatggc attcctcttg ctccatcagc gttcaagagg actgaaccat
    58981 gtcgagaacg caatggctct tgttcggagc tgtgctggtg ggagcagcag tcgcactgta
    59041 tctgatcttc ttctgtccga ccgattgtca ctaacggtcg ctgagctttc acgttgcgcg
    59101 acctgagggc catgaaccga caggatccac atcaatctat ggaagcggag agcatcttcc
    59161 aggtcagaat cagccatgac ggcatcacgc tcgatggaat cctgggtctc cctgcgcacc
    59221 cgaggggtgt catcgccttt gcccacggta gcggcagcgg acgattcagt ccgcgcaacc
    59281 agttcgtcgc ccgccatctg gaaacggaag ggttcgcgac attgctgatg gacctcctca
    59341 ccccggatga ggcggacgat cgccgcaaag tcttcgacat cgacctcctc gccgaccggc
    59401 tgctgctggc agaacgttgg ttacaggcac accgccagac agccggacta cgggtcgggt
    59461 atttcggtgc cagcacggga gccggcgcgg cgctccaagc agctgcacgt gaaccccagg
    59521 cggtcggcgc catcgtgtcg cgcggcggac ggcccgacct ggccggcccc tacctgagcc
    59581 gggtcacggc accgacgctc ctgcttgtcg gcggagacga cggtcccgtg atcgagatga
    59641 atcaggacgc gctcaggcaa ctgacctgcc ggaaagagct ggtgattgtc ccgggagcca
    59701 gtcatttatt cgaggaaccg ggcacgttgg agcaggtcgc ccgggaggcg ctccgctggt
    59761 ttcagcggta tctccagccg gacgcgcacg cagcaaagga gtctcgatca tgaaagtgct
    59821 cttggccgtc gatggatccg atcactccta tgaagccgtg cgggcgctga aatatctgcg
    59881 ccgcccggat gagctgaccc tgctccatgt cgtcgatact ccccgaccgg cctacccgat
    59941 gatggtgccg gaagtcgcgc aggagctgta ttcgcaactg gagcgcagta tgaaggagga
    60001 tggcgagcag ttgctgacca ggatccattc actgctcccg cctcacagcg ggccggtcac
    60061 caaacaattg gccttaggat ccccggcgga aatgatcgtc gcgacggctg aatcacgcca
    60121 tgtggacctc attgtcatgg gagccagggg actgggaccg gtgaaggaac gactgttggg
    60181 aagcgtctcc catcgtgtac tgagcttcgc tccctgcgcc aaactgatcc tacagggctc
    60241 gctccgcgac ttgaaacagg tcttgctccc gctggaagga caatcggacg ccgaggcggc
    60301 gctgcgattc ctccggaagc aacccttcca tgagccggtg aacatcaatc tgctggcggt
    60361 cctcccgccg acccgtccgc cctggcccgt tgacgactcg gtagcggaaa aactggagac
    60421 gcaagccctc aggcatgccc gtgactttgt ggatggagtg gctaccgaac tccggacact
    60481 cgggtatacc acccgtagcg caagcgtcct gggaacgccg gtgacgatga ttctccacga
    60541 ggcggaaaag atgggggtag acctggtcat ggtgggttcg cgcgccaggc agggcatcac
    60601 ccgattcgtc ctgggcagcg tctcgcatgc agtgctgcat cgcgcaccct gtcaggtgtt
    60661 ggtgtttgaa tgagtgccgg agggtaacac gcgcagagga cggctgaaac caatcaggag
    60721 aaacgagata cgcttcacga atgatgaacg tatcgcttga acgttttcag gtgagaaaca
    60781 acaataaaga aagtcaccac aatccccagg ctggtcagaa aggccgtccg gcaaggccgc
    60841 agggaggcgt gcgatcgaga acgccggtgg cggtctttct caacagcctg gcctagcccg
    60901 gactattcat gaatgccgtc gcgaggcgtt caagaccgaa gcccatcggg gcttctcgca
    60961 ggtgaggccg acgcaggcgt acaggcagtc cgtcgaggag gccgaacgag aacagccccg
    61021 ccgggcgaag ggatcggacg acgcagcagg tattcgtgaa tagtccgggc tagcagggct
    61081 gtccgttgaa gaggcagggt ttgtatcgcg cgatatcgaa ctcgatgttc tcctggtaga
    61141 cacgctcgtt cccgttcacc ccgtcctgat ccggatcgtt gaacatcgtg tggtcccacc
    61201 aataaaacag ggggtacggc tcggtgtagt acaccggatt cccgtaattg ctcctcgtat
    61261 actcctgcac cagacgaccc gtaatatagt cgaccttccc gtcgccatgt agcgaataga
    61321 gcatgatgaa cagtcgcgcc tcatggtcgt acagttcatc gaggcgacta ttcaggtcgg
    61381 gctcgatagg aagcggatcc tgtgaccaag cggaggtagc cggaataaag aggagcacag
    61441 cgagcagaag actggcggca acgcgaaggt tcgtcatggt tcagaaagca gatcgacgcc
    61501 atgatgggga caatttcact catgctacca cccagctata ccccgttcaa gcgctcggct
    61561 gactttttcg ggtcctcgtc accttgccga tcgaaggtgt gcgctcttat aatcgcagac
    61621 gatgtccacc agcatgccac gcgcgtcact tcatacactg ggctgtcgtc tcagccagtc
    61681 cgagacctcc atgttagccg ataccctggc gcgccggggg tatcaactgg tggagttcgg
    61741 caaggagacg gacctgctcg tcttgaacac ctgctcggtg acggagaacg ccgaaaaaga
    61801 ttgccgctat gccgtccgca agaccttacg tcactcgccg catgccttcg ttgcggtgac
    61861 cggttgttat gcccagaccg gtgccacgca gctgcagaag gtacccggca tcgacctgat
    61921 cgtcggcacc cagttcaaaa tgaatctccc tgactatctc ccggcgccgg ccaaactcct
    61981 gaaacagccg gagcccgaac tccgccatac ccgtacgatc gatcgcgaag acttcgtcct
    62041 gcccggcacc gcttattccg actcgacccg cgccctgctg aagattcagg acggatgcga
    62101 cttcatgtgc agcttttgcc tgattccgtt cgcacgcggg cgtgagcgca gccggaccgc
    62161 cgaggatgtc ttgcgggaag cccgtgagct cgccgcccat ggctatcgcg aactcgtgct
    62221 cactggggtg aacatcggcc aatactcata tcagggtctc gggctggttg aactcctgcg
    62281 ggaactcgac gcgattcccg acgtcacccg gatcaggatc tcctcaatcg aacctacgac
    62341 cgtccctgct gccttactgg agcacatggc cggctccacg aaactgtgcc attacctgca
    62401 ccttcccctg caaagcggcg acgatgaaat cctgcaggcg atgaaccggc gttatgccgt
    62461 tcgcgaatat gaggagctgg tcgagcaggc catcgcgctg atgccggatc tcggattggg
    62521 aaccgatctc atggtcggat ttcccggcga agacgaacag gcgttcgcga atacggtccg
    62581 gacagtcgag cgactcccct tctcctattt ccatgtcttc agttattcgg cgcggccggg
    62641 tacggcagct gcgcgactgg aggaccagat tccgcctgcc gtcatccggc aacgcagcaa
    62701 ggccctggcg gaactgtcac ggacgaaagc gctggctttt tatcagcaac agatcggccg
    62761 caccctcccc gtcctcttcg aacaggggga acgcgatgga tttcgcaccg gcaccacagc
    62821 caacttcacc cgggtggcgg tcgcggccga tgccgtcgag gccggctcga tccaccaggt
    62881 caccatcacc ggcatcatgg acgggctggc ctatggccgg ccggtcgcca cagtcttagc
    62941 gccctcgcac aggccacttg tatgacacaa cccaataagc cccataccgt ccatatcgaa
    63001 accttcggct gccagatgaa cgagtacgat tccgaactcg tgcggtcgtt actccgcaaa
    63061 gcgggattcg agtttaccga agatcgcgag cgcgccgatg tcatgctcat gaacacctgt
    63121 gccatccgtg agaatgcgca taacaaggtc tatggccatc tcgccgagtt gaaggcggtg
    63181 aaggaacagc gcccgctggt cgtcggggtg ctcggctgca tggcgcaaaa cctcaaagag
    63241 gagctgacgg agaaacaacc gctggtggat gtgctcgtgg gcccggacgg ataccggcag
    63301 ttgccgggac tattgacgaa tgcgctgaat gctgaagaac agggcctggt gcaacggggc
    63361 atggccgtgg atctgtccga atatgaaacc tacgacgaca tcctccccga gcgcgacgac
    63421 agcagcaacg cctggatcgc catcatgcgc ggttgcgaca acttttgcag cttctgcgtg
    63481 gtgccttaca cgcgagggcg cgagcgttca cgcgatcccc agggaatcct ccgcgaggtc
    63541 gaggcgtcgg tcgccaccgg tcatacgcag atcacgttgc tcgggcagaa cgtgaacagt
    63601 taccggtatg aagactggga ctttgcccga ctcatccttg cggtcgccga ggtgccggga
    63661 gtccggcggg tccgcttcac gtctccgcac ccgaaggact ttcctgctgc gttgctggat
    63721 gccgtcgccg gccatccgaa catctgcaaa catatccacc tgcccctgca atccggcaac
    63781 gaccgcatcc tggaactcat gaaccggacc tacagccgga aagaatatct cgatctggcc
    63841 gcgcatattc gacgccgcca tcccgggatc gcgctcacca ccgatatcat ctgtggcttt
    63901 tgctcggaaa ccgaggagga gtttctggat acctatcggg tcgtcgagga ggtgcagtac
    63961 cactccgcct atgtgttcaa gtattcggag cggaaaaata ccatcgcggc gcggaagttc
    64021 ccggacgacg tgccggaggc cgtgaagggc gaacgggtca gccgcctggt ggacctgcag
    64081 cgccctatca ccgcgcgcct caatcgggaa ttgatcggac agaccctgcc ggtgatggtc
    64141 gaaggcgact ccaaacgctc cagcgatcaa tggatgggcc gcaccgatac cggcgtgtac
    64201 gtcatttgga acaagagcga cgccccggcg ttactcggca gcattcaacc ggtcactatt
    64261 ctcgacggca gcgccgcagt cctgatggga cggcgcgagc cctctgcgag agtcgcgtga
    64321 attatccccg cactccgcac aactcatcgc ccggccgcct gcgacaggtc tacgggagac
    64381 caaagattgc cgtcgaacgg cgacattctt gcccagtcct gaacaacagt tgccaatttt
    64441 tagacacccg cactcgtcag actgacggga aaagagcagg catcggcgcc tctcgccgtc
    64501 ttcagcgcct ggctcgcgtg tcggctcacc atcacctgcc tctcaatgga gcgacttcac
    64561 cgccttgaaa cttcaatagc ttagacggca tctttcgccg cgatcaatca cgtcgcatca
    64621 gccgcagcca cagcagagat cgactctcag gggattacca ctagggtctc cgaagtccat
    64681 ggaaagaggg catagccctt gcttggtcca ttctggcgtc cacaattaac caggttcatc
    64741 aaccaccgac cggccccaga ggatttcgca tcatgaagaa agacatcgcc cagagtcatg
    64801 cagaagaggc ccctcggggc atgagcctgg aaaccattat cgccgtcggg gtcttcgggt
    64861 ggatggccct cggcgtggtg atgatgggct tgggaatctg gtaatcccga ttcgttacga
    64921 cgcgccttcg tcctgaagcc gcgccgcctc tgttcgcacc gcatcgacca gccgagaagg
    64981 atgaccgcca tgcgaattca cggctcccgc acccctttca ccaagaccct gaactccgga
    65041 ctcctcgggt tgaccctgtt gggagccatg gctgccggtt gctcatcgac cgccgccacc
    65101 gttcaacaga aggccgctgc gcaaccccag ggttctgtcc ggctcgaaga agtcgccgat
    65161 tgggaatttg aggccagcca ccccagcacc atcgctctcg ccaccctcga tagcctgctg
    65221 cgtggtgtca tgatctcaga cgcaccaatg gatctctcga atctgcccgt ggatggcagc
    65281 aagccgatga aagtattcag cgatgaggac gtgcactatc ttgctcccct gctagcccaa
    65341 gcgttgtctc aagcgcaacc cgaatatgtg gtcgctttcc gtctatcctc atcggcaggg
    65401 tcaggctctg aacccaccgc gggcaccctt tacgttcaga acgaacggtt gtatctcacc
    65461 gtgaccgagc acaacggtac gttggcgaaa gtcgagtcct ccctgttcag cagcgggcga
    65521 ccggctcgca tcgtgagcat cacgccggaa tcagccggaa aaatcgagca ggcattaccg
    65581 gcgattgcgc aaggccggca gccgctcaaa tccctcgcca tcgactacac actgtttgcc
    65641 aagaacccag aaccggcccc ggcggtcgca ggaacgatcg cagcgccggc gcctcaaccg
    65701 gtccaagcct ctgcaccccg gatggtcgtc gcctcggtgg tagaagaggc gccggttaag
    65761 aacgcccccc ctgcgcagcc ggcaggcccg gcatcccatg cgtcgaaagc ttcttccgcc
    65821 gaggatgaac gattgaccga aacacttcag gagttacagg acgcgcggga agcgatggcc
    65881 cggaaagacg ccaagatcag cgccctgcga agagatctgg aatcgatgcg tacgcagttg
    65941 gaaacgaagg acaaggaact tcgggccgtg aagagcaccc cgcagactca acccaaacgc
    66001 gagacccgga acaaggccga actcacggtt cgctgaccgc ctgcccattc attcagccca
    66061 catcgatgac ttccggccgc agaagcacga tctcccgtga gatcggctgc cggaaattca
    66121 tgcggcaata gcagccatac gtgacatagg tatcctgcag gcagtagaga gcgatttcct
    66181 tgccctgccc gtccgcccac atctgggcca cctgcgaccc gctgcccgat ttactttcca
    66241 cgttcaacgc ccgcgcgagc acgtcgagct tgacccaccc acgattgtcc cagttgctcc
    66301 agatcgccat ggtgtcgtac accggctccg cccgaaactt ggccaggttg atctccatgg
    66361 ttggcttcac ctggtgaatg atcgagcgtt tcttgatgaa ggggaggtcg aaattcaggc
    66421 cgttgtgggt aatgaacaac gagggccgga gctgcccgag atgactccag aaacgacgta
    66481 atagctcctg ctcgttcgcc ccgtaccagg aggtggcgcc acgcggttcg aggctgtcgg
    66541 aaaactccag caacccgatg cacacgatgc ggctgaaggt tccgtcgaag gaggattttt
    66601 catagagctc ttcatcgagc cgatgctgcg cctgcgcctc cccgaccgcg aacaggttcc
    66661 ctccggtctc cgaaaaggcc gcctcgcccg aggccagttg acggcccgcc agtcgggccc
    66721 attcctcccg gggagcctgg accgtttcga tgtcgaggac cactttcata cgcccctaca
    66781 ctttcccctg ttgaagcgcc gcaataatgg gctgatcggc cgaaggaaac tcgaacgcgg
    66841 ccatctcatg aggatagacc catcgaagtt ccgcgcagtc cagcgcgatc gcaacaccgg
    66901 tctcgatccg gcagcaaaag aaatgcaatt ccacaatctt ctcgggatat tcatgtcgta
    66961 cgacctgaaa gggagtggga aggccgatac gcacattcag ctcctccctt aactcccgat
    67021 gcagacactc ctccaacgtt tcgcccgttt ctcgtttccc gcccgggaac tcccataagc
    67081 cgccgagatg caccccggcc ttccgccgcg cgatcagata ccggccctcg tgaacaatga
    67141 tccctgccgc aacctcgatc acttccatca tgcaccgcgt ccgggagaag aacgtatggc
    67201 ctatggcatg tagctcggca ctgaaaagca ggtacgtctc ggttccacac tatgggccat
    67261 tggctatgcg ccatatgctc ctgatccgct acctggccgg atcgaacgga taggtcttgc
    67321 agaacggttt catcgggcac aggaggcagt aggggtcgcg cgccgtacag accgtagcgc
    67381 cgaaatccat gagggcctga ttgaagtcat accccttgcc gcgcgggatc agggtttcag
    67441 acaactccca gagggcggcc ttttgcgcct tggggtcgcc ctgagcgatg aacacacgat
    67501 gcagcacgcg gatcacgttc gtgtccagaa tcggagcgtc ttcgttgaac gcaaacgaac
    67561 gaatcgcccc cgcggtgtac cgcccgatgc ccttgaagga caacaactcc tccgcatcgt
    67621 tcggcaactg cccgccgtag cgggctacgg tttcacaagc gatgctgtgc aaccgttccg
    67681 gccggatgtt gtaccccagc ggataccagg tctgcttgac ctccgcgacc ggcgcatcgg
    67741 ctaactgttc aaacgacgga tagcgttcca aaaactcatg gtacttggga atcacacgat
    67801 cgacctgggt ctgctggagc atcacttcag acaccaggat gtgataagga tcggaggtcc
    67861 tgcgccaggg taggtcacgc ccatgttcct tgtaccactt caacaaccgg ttctgaaaac
    67921 gctgcttcag cccgcgtgca agcgggaccg aggactgaga cgacttcttc ttacggcgcg
    67981 gggatttctg aacgcgagac tttgtagaca taacagttcg tgatgagtgg cggcattcta
    68041 agaggcgggc gggctgaaaa tcaaactacg acgcctcgac ggcggaaccg cagagtgcga
    68101 tccacgaaaa caaatccttg cctcgcgccc tgcggtccct ggcagtactc gtgataccgc
    68161 tcgctactgc ggcgaagcct gcaccttggt cccttccttc accggcggat cgatcacaat
    68221 ctgagggcct tgcactttcg gcaagatccc ggcccccggt ttttccagga gtcgctgatt
    68281 ctgctcgtct ttcggaagct gctgcgacac atgacggtta tcgaaagccg ccgccttgct
    68341 gagcgcagac tccccgaccg cattggcacg acccggatcg ttggcgaggg actggccatg
    68401 gatcggatca atcgacttac ccatcggata cccgggatgc tgcggcaaca tggctggatt
    68461 gccgaacgcc atggcagcaa ccaggactcc gctcaccgca acgggacata tgaataatga
    68521 ggcgttcata ccaaacctcc ttttaagcgg gtgaaataaa aacgccctcc tgtccgcacg
    68581 atcaggaggg cgcaggaaga cacctatgga agaactcgga gcgaacaggt gtcgtaggat
    68641 cgaggtcacc ccggcttcat ggtcacctct tggactatgc tgttcagctt gcgctcgttg
    68701 aacccgacaa tcaagtccgc ccaacctcgt aataacatcg acgacgcggg gactagtccc
    68761 gtcgaaggtc ctctcatacc ctagggtttt ccccaactcc aaatggtcgg ggacattatc
    68821 ctgatcgcgc ccacgatggg ggacatgtta tgtccttctg acgaaacacg ttctccgcgc
    68881 tcgttgctca tcccccatta cggagaccac gaggccggtt tccaacgctg gtggcgcggc
    68941 tctacccatc gcctcccgcc ggactataca ggttcgacag gtcgcgcgat gattcaatcc
    69001 acgtaaggat acagaccggc ccatgaccat gaaagttcac tatcaagtgt ctctctcgac
    69061 ggagtccgcc acgagcggag aaggacggct tctggatctc tcggtggagg gctgccgtat
    69121 cgagtgtgcg caccatttgc ccgtcaacac ctatctttcg ctgcgactga tcgtttcacc
    69181 gaaggagatg ccggtcctcg tggatctggc cgccgtgcga tggacgcgtg gaatcgtctg
    69241 cggcatacac tttctctcgc tccagccgtt acagaccgca cggctgaaaa cgtttctcgc
    69301 ctccgccgcg ccacaaaaac caccgaacac acccgacaag cgctaagtca ccggccccct
    69361 ctcttcatct caccccccta ggattttccc ttgccttttt cgtgccgctc ttgcatcctt
    69421 gggccacccc tagagataac cctaggtctc tcgaagaccc atcgactact caagaggaac
    69481 gatgtcatga acgaagagaa tcaaactcgg cgcatagacg tgctggcaat cggcctattc
    69541 ggtctggcag tgggcgcgct caccctgggg gtggcccaac taggatggat tcagcataag
    69601 aacatggtgg gagccctggt gatcgccctg atcttcggcg ggatcgttca attgctggcg
    69661 ggcatcaccg atattcgcta caacgagcaa ctgggcggaa ccgctctgac aatgtacggt
    69721 ttcctctgga tcacgctctg cacggtcaaa ctggtcggca ccagcggcac cctccagttc
    69781 gatgcggtac tctacgcgcc catcaatctg gtctacgcgg tcttttccgg cgtcatgatt
    69841 tttctcacgg cctaccgtaa cctgaccctc agtgccctgc acgtggtgat taccctgacg
    69901 tttctggcca ccaccttcgc ccggctggac ttcatcagcg agctcttgcc gggatggggc
    69961 cacatcattg tcggcctgat ggcgttttat catgccgtcg gcagcctgac gcaggccttt
    70021 accgggaaat ccatcctccc gctcggcccg ccgctcctgt ttcacacaca gaagcacgtg
    70081 cactcgatcg ccaaggtcgt gtagcctggg tgacgacaag cagccgacta cgatgacgta
    70141 tttcggcgcg cgttattcgt aggctgttca aaaaggctgt ccagcaaggc cgcagcgagg
    70201 tccgcgacgc gaagaagaat gagcgtcacg tctgcggacg ggcgcgagcc ggtgagcgcc
    70261 cagtatccta gaggcgaatc gctctttctt acccgcccgc cccgagccta ccaagacagg
    70321 ctcttgtccc atgctcgtac ggtgagcctc tgatcgaagc gagaacgacg ctggcagact
    70381 ttttcaacag cctactacca gtccgcgccg atccggtcca ccacgatctg atgcacggcc
    70441 gactggggac cgaggcaggc caggtagagc gcggtctcgg cgatatcgaa cggatcgatc
    70501 ttttcgctgc cggcccgctc cccagccgag gcatcgacca gatcatccgc cacgccgccg
    70561 ggacagatcg cgctcacttt gatacggtgc gcgcgcccct cgtccgccag tgccttactc
    70621 aacgccatga tcgcatgttt cgacgcgctg taagtccccg tgccgctcca tgcctgcacg
    70681 cccgccacgc tcgacatatt gagaatcgtg ccgccgccct gcttcttcat ctgcgcaaag
    70741 gcggcccgac agcagaagaa cgtcccgcgc acattcgtat tcatcacttc gtcgaaggct
    70801 tcggtgctgg tctcagccag ccggcgtccg ccgaagatcc cggcgttgtt caccaggata
    70861 tccaggcgct ggaaacgccg aaccgtgtca ccgatcagtc gctccacctg cgactcgtcc
    70921 gccacgtcgg tctgaagcgg cacggcggtt ccaccggccg cctcgatctg cgcgacggtt
    70981 tcctcgcatt tatacattcg tctggccgca acgacgaccg tcgccccttc ctgcgcaaac
    71041 cggagcgcga tggccttgcc gatcccgctg ctgctgccgg tgacgatcgc gactttgccg
    71101 ttcaagcgtt tcatagctcc gacctcctca atgaattgct cctgccggac tgagagccgc
    71161 ctctaccata cagggaaacg gattgcagaa ataatccctg acgtgaatgg atcgaagcgt
    71221 gtgaggtcag gcggcgggtc gcgccggcac agcctgccag cggaaaagca gctaacgggg
    71281 atttttctcg gtcgggttct ctacacgcga ggtatgacga tcgacatttc gcccaagtac
    71341 ttcgccgact cgtgccagca cttcctgttc ggccgcttcc cgtgtggagc aaggccctga
    71401 ggcgaccgat tgctcggcaa ggttgtgccc caccccgcaa ccgacggtgg catccgcgga
    71461 ccagcgacca tcaccggcgg catggaatgt cacatggacg tcacacccgg tgatgcggat
    71521 atccttcgtg tcattcgaca tctatcaaac tcctcccctg ctggtagaaa ccggaatgca
    71581 tgaggcacag gttcaaggta atcctgcaat cggaaaccgc acactaggag gaccccctgg
    71641 atgcagaggc acgctctcag agtcgagcat ggacgaaacc gaaggcgtta tgtgtctccg
    71701 agatgatgtg agacgaatgc atgatcgccg atcgttcctc gagccaaatc cagcagatgc
    71761 cgatggtgcg tgaacagcat gacctggctg ccctcggcga atcgcgccag ggcccgcagg
    71821 atgttggccg cacgcgcgtc atcggatgtg atgagggtat catcgagcac cagcggcatc
    71881 ggcggatggg aggggcgccg caactccaac gccgccagcc gtagcgccag gtacaattga
    71941 tcggcggtgc cttcgctcat ctcctcgatg ccgatcgtca caccgccggc ccgttgcgcg
    72001 cacagaaccg gcctgtcctc gcgctcgtcc gtcaccagcc gttcgtaggc gccgcccgtc
    72061 atcagcgaaa agtaggcaga tgcggcggac accatgggcg cctgcgcccg ttcacgaaac
    72121 cggttcaacg cctcctgcag caacgctcgc gcaagtttca gcctggccca gggcctgatg
    72181 gccgagcggt atcgcgccgc cgcagattcc atcgcttcac gcgccagcgc cgcgcgatcc
    72241 gacgcgtcga tgacttccag cgcgcgtctg gcctgctctt ccgtccgttg cgccgaggtc
    72301 tgttcctgct caagccgcgc cctctcggta cggcaacgtt cacgctcgct ctcgagggcc
    72361 ggcacatcca gccccgtgag gcgtgcccgc agctctgctt ccgactgagg cgaagcctga
    72421 gccagttgct gacgcaggag ggcgagtgat ttccgcacct cacgtttgtt cgccgcctgt
    72481 tcttccaatt ccggcagctg gtcgatcgtc gctcccatcg cgcgcgtgca gagtccggcc
    72541 aacacggcgg cctccgtgac ctgtgcggcc gcagcctgtc gcttcttctc ctgagccttc
    72601 ctgcggtcgc gaatgagcgc atgcccttcc tgttgatgtt gccgcgaaac agccaaccgt
    72661 ttgcggagac gatccgcaaa atcatccagc accgtgggaa ctgcctcacc gagtaattcg
    72721 gccacctgtg cgccttgtgt catgaggtca tcgaccacgg cctgtaggtg cgcctttcgt
    72781 tgttgcgcct ccgctagggc cgacgcctgt cgtgccagcc catcgagttc gtcgagccgc
    72841 gacttgagcg cctccggcgg tgccccaccc gacaagaaca gtctggtatg ccaggactgc
    72901 aacccgtcca gatgacggcg acgttcagcc tcggtctcgc tcacctgccg gccgaccttc
    72961 tcccgctcga tccgctggct atgagccgtc ttgagacgag cctgctgctc ggcctccgct
    73021 tcggtcgccg attgttccca acgcacggcc tgttcgatca gcgcggagag ttgttccgcc
    73081 tctcccgctt gcgtcgcggc gggagaacag ccgacggcac gcaacccttc aataattgct
    73141 ccgacggagc ctgcggcatc ggccagcaac cggtcgcggt cggcacgcag ggccgtcaca
    73201 cgctcaacca gctgcagcgc ctcatgccgg cgcccctgcc attcacgcaa cgtctcggga
    73261 tccaggtccg gcaacccggc ttgcgtcagg cgctgccgcc aggctccttg caacccgtcg
    73321 cgtttcgcgc gcagcgcagc catctcaccg gccagttcct tgcggcgcaa ttccatctgc
    73381 tcgatgcgca ccgagcactc ttccaatcca gccgcgcgtt tggtgtccgc acgcagtagg
    73441 tccgcctgac ggtcggcctc acccaccgcc gcttcgtacg tctccggcaa ggccttggtg
    73501 gcgtcgaacc ccagcgccag ctgatccgtc ccgccggtcc tatcgatgta ggtgcgacga
    73561 atcgccgtcc attcctcgga acgtttggcg cgggcctggc gcaacgtctc cgccgtcacc
    73621 acctcacctt ctgcagcgag ctgccgttgc cgcagccgtt gcccgtcgag gtcgcgcccg
    73681 accagttcgg attcgtcacg ccacttggtg agctgtacct caatatctgt cagatcctgt
    73741 ttcgcctgcg cgatctgagc ctctaaaagc ggttggctgc gacgcaacgc ctgctccgat
    73801 tcgagaccga gttcggaaag cgccagggtg agccggccct ccagttcacg aagccggcga
    73861 tccagctcgc tcttctgcct gaccacatca ccctgtgcct gccccgctcg tagtgccgcc
    73921 gttaacgcct gcctgtgcag tggatccggt actgtatcgg gcagcgcgct ggccggttgc
    73981 aaggctgcat ccaacgcttc ggcacgctca cgatagcctt caagccgctc gcccaatcga
    74041 ctcacctcgg caaggtgccc ctccaacgcc acacggtcgg cgacggaagg cacggccgct
    74101 agaatggtcc gccgatccac acccggtgcc agtctggcca caaccagatc caactcgccc
    74161 tcgatcttcg caatgaggct gttctgctga tgaacttcat ggcgatttct ggccgcggct
    74221 tccacgcctg agaccaggcg ttcaatcgcg tcggcatggt cgagcaacaa cggttcgatg
    74281 acgagtccat ccagcgcggc ggtgcaacgc gcgagctcct cttcggcttc tcgaagatct
    74341 acctgcgcgc gctgcaacgc ctgttccgcg gccagccgtt cctcacgttg ctgctcggac
    74401 agatcgggaa ccgccgcata ggactggagc gcgcccgcca ggcgatcatg ctcacgcagc
    74461 aacggttcaa ccgtgcgtag ttcggtcaat tcgttctccc gccgacgcaa cgtttccaag
    74521 gctttggtga ggtcgtcgag agccgccttg gcggtttcat gcgcgcgatt caacgcctgc
    74581 cactcggccg gtttggtctg cgcctcgcgc caggctttcc gctgctcgtc gagctgacgg
    74641 cgcgcctcgt tgatcaccgc attctgcgcg cgaccgtgct ggctatacag ttttttggca
    74701 tcggcatcga gggcctccag caacgccgca atgccgctgg tgcctgcgct cgcctcgaac
    74761 aatgccgagc ccaggtcgcc ttcgccgctc agcagaacgg cgccgccctc gcgaagtcgc
    74821 ccatgattca atccgaacat cgcttcgaat tcccggcgtt ccaacccacc ggtcagttcc
    74881 cgctccaacc tgctgtccac ggcaaaacca ggatccgtct gttccgtgcg gacatcgagt
    74941 ccagagaggg tcgtccccct gcccttgcgc ctgatgagtc cgaccggtcg gcccgtctgg
    75001 tcgatgaaca caccgccgat ccgcaattcg ccggccgggt ggacgaagtc gtccgggcta
    75061 cgcagtggaa tgccgaaacg taagtccgtc atggcccgca gggccgacga cttgcctgct
    75121 tcgttgggtc catagatcag atgcaaattg ccggagccgg tggaaaagtc caacgtctta
    75181 ttggtgaaag ggccgaaggc gagcagcaac aggcgcgcga gtttcatgcc tcttctccgg
    75241 aggcggacag gcgcgccaag actgtggctt ccgcatccat caggagactt cgcaattcgg
    75301 caggatcgtc gagacgcggc acatcaccgg ccgtgacttc cgcgggcatg gcaccgagga
    75361 gatctcccag ttcgacctgt gcccgacgca tcagttcctg ctcgttgccc gcaatcgtat
    75421 cgaccagacg gaccaactct cccaccgcat cctggcgctg cgcagcccgg gcacgatcca
    75481 gcggggtgga aagatccagc cgtacctgct cgatccagat ctccgccgtg ccgatgtcct
    75541 gcgccgccgc atgcatcgcc gccgcgagcg tgcccggctg tgccgcctcc aaccggtgca
    75601 actcggtcga tcccgtcagc gtcacgcgca ccgcatgcag ccgatccgtc gtcccgtcca
    75661 gcaccggttc caacgcgcgg gcgaaggcct cgttgagcga ttccaaccgg tccacgccat
    75721 cgagcggcac ggagagctga ctccaacgaa ccacatccag cgcgataaac tcggcctcga
    75781 tcctccctgc ttccaccgtg accaattcgc accccttcac gcccgtttcc ttcgcatgac
    75841 ggccctgcag gttgccgcaa aacacgatgc gcggccgctc gttcagcacc tcacgcgcgt
    75901 gcacatggcc gagcgcccag tagtcatacc ccttggcaac cagcgtcggc aggtcggtgg
    75961 gcgcataggt atcgtggccg gcgcgccccg tcaggctcgt gtgcaacaga ccgatattaa
    76021 aatagcccga aaccggcggg gggtaagacg gaaccaggtc ttcattcacc tcgcgctcag
    76081 gaaagctgcg gccatggatc gccacggaga ggtcatccag cttgaccgtc tgggctgcgc
    76141 ggctcgaaaa gaccgtcacg ttcgacggca gggtcaactg gcgcgagatc actccctgcg
    76201 catcgtgatt tccctgcacg atgaagcagc ggatgccggc gcgctccaat cgcaccatct
    76261 gcccgcgaaa gaagaggccg gtgtgaaaat cctgccagtc ccggtcgtag atgtcgcccg
    76321 ccagcaacag gaaatccacg cgctcggcca acgctttatc taccaaccgc tccagcgcac
    76381 tgcgagtcgc gctacgcaac cgctcaaccg gcgcaccatc gtagcggtcc aggccgcgta
    76441 agggactatc gatgtgaaga tcggaagcgt ggatgaagcg catgagaatt aggtcctgcc
    76501 ggtttctttg agaagtgtca atcgaagcgg cctaccgcca aggcaattcg agtcaatata
    76561 cgatccctca tataccacat catctccatc gtcgcacttg agtcgcatat gccacgaatg
    76621 cctggccgaa taggcgggtg agcgcccttt cctccggttc gatttggaaa cgattcatag
    76681 agagggcgaa gccgggaaga accaggaaag ccggcgcgtt cgagaggaat atgccccagc
    76741 cgggcaagat ccacaagaag cccagataca tcggattgcg cgtcagtcga tagatgccgg
    76801 cacgaccagc gcggacgagg agtccggctt catctgtgtc cccgcccgcc acagtccggc
    76861 gggcggggca caagcgccct accgacccgg agtttgcttg cggcaaccac attggcaatt
    76921 ggtttgctcg cagcgacagg cgtttgcctg gcatccgcag gcacaggatg agcaatcgca
    76981 ggttccgcaa cagcttgtct tggcaatctt ctgcgcggtg gtttttttca tttcggccat
    77041 ggtgggtctc ctttgagatg ccgacgcgtg attgcgccgt cgcagaggct gacgaagggc
    77101 caccggaaac cttacagttc cgcccacagt ttggtaagct gctcccatga acctcgaatc
    77161 cgacccctca acagcacttt cgccggaggc catcgcgcaa ctggtgaaag gacaccgtga
    77221 gttcctggcc ttcctggaac ggcgcgtgga atctcgcgcc gtggcggaag atattctaca
    77281 gacggccttt acccgtggat tggagcgcgg gggcgacgtg aaggacgaaa aggtcgtcgc
    77341 ctggttctat cgggtactcc gcaacgccgt gatcgatcac taccggcagc ggtccaccac
    77401 cgcgcgagcc atggaagcct gggggcgtga atttccggat gtgcaggagc ctgaagcgga
    77461 gctgcggcag gagatctgcc gatgcgtctc gggcctgctg gaaacgctca agccggaata
    77521 tcgcgaagc
//
LOCUS       DAORRQ010000013        77398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_13_length_77398_cov_174.266941, whole genome shotgun sequence.
ACCESSION   DAORRQ010000013 DAORRQ010000000
VERSION     DAORRQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 77398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77398
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13_length_77398_cov_174.266941"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..736
                     /locus_tag="PK224_05985"
     CDS             296..736
                     /locus_tag="PK224_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRB15319.1"
                     /translation="MFAGEYLCKVDDKGRFLIPSPLRERLEAEGNQVMFVKNTEQTLW
                     MYSAKEWEKVLERTRITLDEDQSRLFMHHVMSQAGTSDIDKAGRVLIPSRLRKLVPMD
                     EDQEIFLVGMYHRLEVWGPSEWRRYLTRTEDRYEQDMAKIQNLL"
     gene            890..1399
                     /locus_tag="PK224_05990"
     CDS             890..1399
                     /locus_tag="PK224_05990"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HRB15320.1"
                     /translation="MAITSAPPSVRVTADSIALTLPVPPSVNHQYATVNGRRLLSAKG
                     RAFKELVGQQILVALAQSPHRGTLRRTLSQASLSLSIQFFFASALRRDTDGGLKIAQD
                     ALCEGLGLNDNRVVETHLYKYQDRDNPRMEILLSVATPAAPVSACPASREAPLVVNPD
                     HRSDSRRER"
     gene            1452..2690
                     /locus_tag="PK224_05995"
     CDS             1452..2690
                     /locus_tag="PK224_05995"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HRB15321.1"
                     /translation="MPYRPITIDALQIGMHVAKLDVAWFRSPFMRHSFLIRTDEQIEK
                     LRRAGVKHLSIDPAKGLDLPGTPASTPNKPNVVVQPSSTPTSSTPEVRSLAAMTQELL
                     TARTARAQLEASVQTTFSRIAKTGIVDPEEATHTVHAISAVAQALNSHALFMAFSQGR
                     EGHAPLSQHALATCSFSMILAHAADYNLMAIQELATGALLHDIGLMQVPPNILQRIHD
                     TSTTLSEQNRRTYEAHARGGAILLERRDGFSPAVEQIVAEHHAYLNGSGFPAETGGAF
                     TSDMTRIVMVTDRYDELLTGFGGASPLTPHQSLQRLYQEGQEGRYDTRLISLFVKVMG
                     IYPVYSYVSLTTGERAIVSVINSGKLHQPIVTITHDPSGEPYIVPLVIDLANQDTEAP
                     PRGIRSVLGTIPAEFERAFH"
     gene            complement(2763..3998)
                     /locus_tag="PK224_06000"
     CDS             complement(2763..3998)
                     /locus_tag="PK224_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB15322.1"
                     /translation="MPAFILLTVMTALRILSRNRLRAGLTMLGIVIGVGAVIAMVSIG
                     QGARAAVQAQVASMGTNVIIIMPGSTTVSGVRGGQGGAVTLTVGDAADLKKRSPLLSD
                     TGWAKRDVMQIVNGNRNWNGPVNGISPSYLTIRDWSFTSGGPFTHTDLEGAARVALIG
                     QTTLENLFDPGEEAIGATIRIKNVPFQVVGVLAPKGQSAQGSDQDDVIFIPFTTAERK
                     VFGSQFLGSVGALFASTEQSADLPEAVDQIREVLRARHRLQGEQADDFTIRTQVDIGK
                     VQESTSQTLTVMLFAIASVSLLVGGIGIMNILLVSVTERTREIGVRMAVGAKRLHILM
                     QFLIEAVTLSLFGGVIGVVVGIVGARLTTVIAGWPTIISFDAIVTAFVFSLAVGLFFG
                     LYPANKAARLNPIEALRYE"
     gene            complement(4006..4716)
                     /locus_tag="PK224_06005"
     CDS             complement(4006..4716)
                     /locus_tag="PK224_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB15323.1"
                     /translation="MASLIVCEDLWKIYRVGDVEVQALRGINLTIDRGEFVAVMGTSG
                     SGKSTLMNILGCLDQPTHGRYQLDGLDVATAKPDLLAELRNRQIGFVFQSFNLIPRTS
                     ALENAQLPLFYRGLSIKEQRKQAAAALQRVGLAGREQHYPTQLSGGQQQRVAIARALV
                     GAPSILFADEPTGNLDTASSREIMNILEELNREDGITIILVTHESDIAAYASRELVMK
                     DGQIVQDVRRAPHLTVVQ"
     gene            complement(4729..6003)
                     /locus_tag="PK224_06010"
     CDS             complement(4729..6003)
                     /locus_tag="PK224_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB15324.1"
                     /translation="MLRIGVIVGVLALGLAIAGYVFFNGERKAVVRYRTVPIERGTIV
                     SLVTATGTINPITTIQVGSQVSGMIESLHADFNSKVTANQVVARIDPFPYQARRDQAV
                     ASLANAKAAFDKARIDLAQRRRELDRAKSLIGQQFISQNEVDVALTASEGAVAQLKVT
                     EAAVKQAEAMLQAAELDLKYTVIRSPVDGVVISRLVEVGQRIAASFTIPMLFLIAEDV
                     TKMQVDTNVSEADIGGIAEGKAASFTVDAYPGEQFQGRVRQVRNAPINIQNVVTYDVV
                     VEFENPAFRLKPGMTANVSIVVAKKENILKVPNSALRFTPPKTVRDEQASGRPTKVEG
                     GGRMSAGGTSDAATRQLAIWKQGADEALERVPVEMGISDGAYAEISSASINEGDQVII
                     GIDSPRGDRKSGDLPPGFGSGQQRGPRRDRGL"
     gene            complement(6193..6921)
                     /locus_tag="PK224_06015"
     CDS             complement(6193..6921)
                     /locus_tag="PK224_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15325.1"
                     /translation="MRHVWMAGLALMSAMAVSGCVSDKKYHEALADADTVRGDLERAR
                     AQKNALEQQVKTLKDLNAKFGSESQIARDELQRIQHGRDKERDTIDVRTKELEDKVKQ
                     LSAQNRNIKQEYDEARRHNEQLKSLVARYQKEMKERSRSLSSSAPAELTAPLSGGASA
                     AKSASSPTPFGGPVEAAAPPVSLLNINKAPSTDLVLTLGVSQDVADRIVSNRPYRVKG
                     ELVAKNVVPKDIFDDIKDRITVSP"
     gene            7175..8050
                     /locus_tag="PK224_06020"
     CDS             7175..8050
                     /locus_tag="PK224_06020"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRB15326.1"
                     /translation="MMNWGPELAIILGIIEGLTEFLPVSSTGHLILVGHALGFTGDIA
                     SNVEISIQLGSILAIIAYERTKLASLASHAFREQRDFRSLVAARGNASWATLIQQSIQ
                     AQPNLWFVIGLGLAFLPAALVGFLAHKSIKAYLFSPTTVAISLIVGGIIILVVERMRD
                     RVRVKELLQVTPRLAIWVGIAQCASLIPGMSRSGSTIVGGLLAGLDRRVATEYSFFLA
                     LPTMIIATIYQMLKSQAAFSQDDYVALGIGLVVSFAVAWAVIAAFLTYVQRHSLSVFA
                     YYRMVLGVIVLLVVR"
     gene            8080..8346
                     /locus_tag="PK224_06025"
     CDS             8080..8346
                     /locus_tag="PK224_06025"
                     /inference="COORDINATES: protein motif:HMM:NF021169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HRB15327.1"
                     /translation="MANQMDSVHVYDTWVKGKNGKLHFDVMTTSQEKALVLAKQYLVG
                     IGEPNAVITLKECQFCHSEPLVMFSEEQQRQFREQGGFIITMPV"
     gene            complement(8506..9027)
                     /gene="lspA"
                     /locus_tag="PK224_06030"
     CDS             complement(8506..9027)
                     /gene="lspA"
                     /locus_tag="PK224_06030"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRB15328.1"
                     /translation="MSNSVRYLLLGLLSLAIVVLDQVTKLSVMESMRLHESIPVITNF
                     FSITYIRNPGAAFGFLSSSSSSFRFIFFGVTSIFAVGLLGTILVRMPKDDWMGRLSVA
                     AILGGAIGNLLDRLRYGEVIDFLDFYFNAYHFPAFNVADSAITVGVAFLILHFALEKE
                     PEATPVVPENPPS"
     gene            complement(9024..11843)
                     /gene="ileS"
                     /locus_tag="PK224_06035"
     CDS             complement(9024..11843)
                     /gene="ileS"
                     /locus_tag="PK224_06035"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRB15329.1"
                     /translation="MDYKATLNLPKTDFPMKANLPQREPEMLARWTQEKLYERIQESR
                     QGFPRYVLHDGPPYANGRIHIGHALNKILKDIIIKSKTMSGYQVPYVPGWDCHGLPIE
                     HQVLKELGDKKRDLDALAIRKLCKEYAEKYVSIQREEFQRLGVLGDWQQPYLTLNPAY
                     EGTIIREFGRFVERGGVYKGLKPVLWCTQDQTALAEAEVEYDDHTSPSVYVRFPLVSP
                     PAVLSKRFGGLAFPAEVKSASVLIWTTTPWTLPANQAVCLHADIDYAFVQVGDELLII
                     AEKLVEAVAKACSLPAYKVVGVKKGAEGFEGLETQRPLTTGLSPLLLGDFVTLEQGTG
                     CVHIAPGHGMEDYLLVLDHNAKASVGERLEILAPVDNGGKFTAAVPEFVGQHVFKANP
                     KIVERLKENGRLLGHGTLNHSYPHCWRCKQPVIFRATEQWFVSMETNELRKEALAEIE
                     RVRWIPAYGRDRINGMIENRPDWCLSRQRVWGTPIPGFTCVECGKVLAHPRVIDHVAD
                     LIGQHGTDYWFANQAAALLPAGTQCEGCGGTEFEKERDILDVWFESGVSYAAVLKSRQ
                     WYPADLYLEGSDQHRGWFHSALLAGVITDHQAPYKAVLTHGFVLDGAGRKMSKSAGNV
                     VAPQDVIKQSGAEILRLWVSAQDYREDLRISQEILNHLIEAYRKIRNTCRFLLSNLYD
                     FDPAQHRVPFEQLPELDRWALMRLGDLIPRVRKGYDDFEFHTIFHALNNFCSVDLSAV
                     YLDILKDRLYTFRKDSPLRRGSQTVLFDILVALTKLMAPVLSFTAEEIWRMLPESVRV
                     DSKADSVHLAMFPEVDPRWADAKLAGRWEQLLEVRTAVQAALEVQRREKVIGAPLEAK
                     VLIAAHPAHYDLLKTYGQDLPAFFIVSDVVLQSAPDLPEKPGFVITVEKATGVKCERC
                     WNYRGSVGTSSDHPTLCDRCVEAVR"
     gene            complement(12107..13210)
                     /gene="nadA"
                     /locus_tag="PK224_06040"
     CDS             complement(12107..13210)
                     /gene="nadA"
                     /locus_tag="PK224_06040"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRB15330.1"
                     /translation="MKTVATLPRPITDYQSLSAEELFERTRTAKRTLGDRVMVLGHNY
                     QRDEVIQHADFRGDSLILAQVAEQRSDRPYVVFCGVHFMAETADVLSRSNQTVILPDM
                     AAGCSMADMAAIEQVEQCWDALGRVVPVEETVMPAVYVNSAAILKAFCGEHGGLTCTS
                     SNARKVIEWSWARREKILFFPDEHLGRNTANKMGIPREQMIVWDPFMPRGGNSVEAIQ
                     RAKLILWKGHCSVHQMFQPSHVDYFRKQYPDVKVIVHPECHEDVVNKADLVGSTEYII
                     RTITAAPAGTTWAVGTELNLVNRLKHEQTDKKVFFLSSTVCQCATMYRIDAPHLCWAL
                     ENLADGHVVNRIVVPDDEKQWAKVALDRMMALS"
     gene            13650..15893
                     /locus_tag="PK224_06045"
     CDS             13650..15893
                     /locus_tag="PK224_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HRB15331.1"
                     /translation="MNISPDRPIELIKNDALGRKPFVQSVAAAISGWRGNESLTIAIS
                     GPWGSGKSSVKNMIRDSLSLSQSAVQVAEFNPWQWAGQAQLAEAFFREIEIALGRSPS
                     ADSRKSAAKWRLYSAKLRIRRLVTQTASNMITRVLSLIVGSGLAWAVISKHLIWTIVF
                     LLFTGIIVAGGKVFDALGEFASSWAQLKEAQAAVHETSTEDIKGELRQLLKKLSAPIL
                     VIIDDIDRLTAPQIGLLFQLIKANADFPNLIYLLLMERAVIEEALNSQASGNGKYYLG
                     KIVQVDLDLPTIPRGKLIEMLEREILAMLDYSPTIDSDEEDHRLSSALTAISPHVKTI
                     RSVHRFTNTLAFDLSSLRGDASLGVNKIDLLCLAAVKVFHPQVYRQLPKEKTLLTESH
                     FPDYQRKSVNRLNAFVLSQTSPDEQSYLLALLAELFPNFPWKMEEEEDLFTHHLSSRT
                     VTENEALTTLSICHHKNFDRYFEQTIQDGDISAEEFDELIEFLPNQAKTYSRLCDFHQ
                     RGILLSVLERLLACVDNFDSGVLTPLLTALFDISDECLPEWESAQRPPTDRLLLFLSS
                     SILQSIQDRTERLETFSKAVETSTGLYLPVSKVALEEPGSKRQAVGGLSETELAPLQR
                     NCLNRIKSWAEAGKLINHPALGYIILRWQEWSQDETCRSWVASQINSKEGLIRICTSL
                     ENRHYDQNGMTYSIRLDLLEQIADLGHIVHKLKALNYDALTNKEKQGVDRFHLAMQQR
                     LKNEHAS"
     gene            16071..16205
                     /locus_tag="PK224_06050"
     CDS             16071..16205
                     /locus_tag="PK224_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15332.1"
                     /translation="MSGLAITHTKVILLGGSTQQRQQQAIETARDRWADYRRRKGTRT
                     "
     gene            16224..16544
                     /locus_tag="PK224_06055"
     CDS             16224..16544
                     /locus_tag="PK224_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRB15333.1"
                     /translation="MALTRQFKDTVTARAERDPRFREALFAEALNAYFSGDTTVGKAI
                     LRDLVNATIGFEELALTLKKPSKSLHRMLGPGGNPSTDNFFSILNALQKKARVTLRVT
                     AKAS"
     gene            16595..16876
                     /locus_tag="PK224_06060"
     CDS             16595..16876
                     /locus_tag="PK224_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRB15334.1"
                     /translation="MPHVLIIHEVEAYPAWKAVFDGAADLRKRAGEISYQLLRYDQDA
                     NSIVHFSAWSSLENARRFFESLELVELRRAAGVKAPDFLYLEEIERGVL"
     gene            17084..17536
                     /locus_tag="PK224_06065"
     CDS             17084..17536
                     /locus_tag="PK224_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB15335.1"
                     /translation="MTHTVRQATVDDVEQLVPLFDAYRQFYGRTSDVAAARAFLLARF
                     ANKESTLFIAHEGDTAIGFAHLYPSFSSVSLARIFILNDLFVQEQARRTRVASSLLSA
                     AATFAVSLGAVRLSLSTALTNDAAHALYRSAGWNRDQQFSVYHLALSV"
     gene            complement(17642..19894)
                     /locus_tag="PK224_06070"
     CDS             complement(17642..19894)
                     /locus_tag="PK224_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15336.1"
                     /translation="MKEQDQIDQEYRVEAAEIARPGIEPLIPILPWPPRVRVSGLYAA
                     SRIVTPPIPAPGPLPIQPVPLAQPMPGIAPTAAGPWQIEELLPWGFLREELRLDVDGR
                     YPQMMASGTLYGVLAKRVHWIARLTASGPNAWTGSIWFKDGDVASFPYTMVEITTTAT
                     FLSSQRKAVVRFSGGGTSVRTRTLAFKSPYFRQVEFEFDCATGVTATTHIGTHDHPNR
                     PATLPGETLTIEEVYRRVGFDVRKSGSDSIVPIGDAGTNARWSDNEMHDAMQVYWSRF
                     AGKAQWSLWVFFARLHEQGTSLGGIMFDDIGPNHRQGTAIFNESFISTAPTGDANPVA
                     WAKRMRFWTACHEMGHAFNLAHSWQKQHPPDWGTPWIPLANEPEARSFMNYPYNVSGG
                     QTAFFSDFAYRFSDNELVFMRHAPERFVQMGNADWFDHHGFEQASASPEPALKLNLRV
                     DRAQATYQFLEPVVLELKLTNIGSRPLVLEKELLSMTDHMTVIVKKRDKPARQYLPFA
                     RYCHDMQAQVVMPGEFVTDSLFISVGRNGWDIAEPGYYTIQIALHMETEDVVSEALTI
                     RVAPPRGYDEESIAQDFFSDDVGRILNFDGSAILRRGNDTLREVSDRFGDRAVAYHAR
                     VALASPLAKDYKVLDMGDRMGEMASAKEAGGRLRRAAPRIEEARQLYTSALLEKKDQA
                     IATLGRTDYEYYLGRFRESLMEHGAVLKKEPRDVKRDAKREKNGDIEHTMRVIKETLS
                     SLKDQEPVSR"
     gene            complement(19957..20256)
                     /locus_tag="PK224_06075"
     CDS             complement(19957..20256)
                     /locus_tag="PK224_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15337.1"
                     /translation="MLLHIALQEGFHHDEVRVSVDAREVWGSADLTTRRQTGYAAAVE
                     VDVAHTPVVVEVVLSKRGVAQTKSVSVDRPTYLGVSLTTEGNLAWKTSYEPFGYL"
     regulatory      20833..20936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            21028..22944
                     /gene="thiC"
                     /locus_tag="PK224_06080"
     CDS             21028..22944
                     /gene="thiC"
                     /locus_tag="PK224_06080"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRB15338.1"
                     /translation="MSEAHTQNGSSNGHGSKPSTARLTTTPFAASRKVHVDGALPGVR
                     VPMREISLTPTQSANGQGPTPNQPITIYDTSGPYTDPNVTIDVRAGLPPLRRAWIESR
                     RDTEELSQVTSQYGRQRAADPKLADLRFTHIRKPLRAKAGHNVSQMHYAKKGIITPEM
                     EFIAIRENQSRARAKELLASTNGHGGGVLQHPGQAWGARIPNIITPEFVRDEVARGRA
                     IIPNNINHPETEPMIIGRNFLVKINSNIGNSAVASSIEEEVEKMIWSIRWGADTVMDL
                     STGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTAKRMTGIVSRGGSIHAKWCLSHHQENFAYTHFEEICEIMKAY
                     DVAFSLGDGLRPGSIADANDEAQFAELETLGELTKIAWNHDVQVMIEGPGHVPMHMIQ
                     ENMEKQLKECQEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHL
                     GLPTKEDVKVGVVTYKIAAHAADLAKGHPGAQARDNALSKARFEFRWEDQFNLSLDPD
                     TARSYHDATLPDNAAKVAHFCSMCGPHFCSMKITQDVRDYAAQKQLEEQQAIQIGMKE
                     KSEEFKKAGSEIYL"
     gene            23084..23893
                     /locus_tag="PK224_06085"
     CDS             23084..23893
                     /locus_tag="PK224_06085"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HRB15339.1"
                     /translation="MGKPKPAPLRERDITRQIAREYYKEFDQLIESDVIIVGAGPSGL
                     ICAHDLGRMGIKTLIVEQSLALGGGFWSGGYLMNKATICAPAHKILKEVGVPCKQIKE
                     CPGMYMVDPPHATGALIAAAYNAGAKIMNLTRVVDLILRREGVLEGVVVNSTTAEMAG
                     HDIIHVDPIALESKIVVDATGHDAVVVNLLHKRGLYQQVPGNGAMWVSRSEEEVMERT
                     GEVSPNCFVIGLAVAAVFGTPRMGPAFGSMLLSGRYGAELIQKKLKQGKSI"
     gene            23973..24488
                     /locus_tag="PK224_06090"
     CDS             23973..24488
                     /locus_tag="PK224_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB15340.1"
                     /translation="MDVQDFLIQGDGSEEDKREAWQLFQQAYEQQMKGQLEEAVSLYK
                     LSLATHQTAEAYTFLGWTYSFMGKLDEAIDECHKAIACDPHFGNPYNDIGAYLIEKGD
                     LEEAIPWFQKAMQARRYESPAFPHLNLGRVYERQGKWSEAIDCYKQALALNPDYALAK
                     KALGRLISSLN"
     gene            24510..24923
                     /locus_tag="PK224_06095"
     CDS             24510..24923
                     /locus_tag="PK224_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRB15341.1"
                     /translation="MNTTILVAVTDIFFYTKVRDALRPQGYTLERIRSQDEVAEKTAS
                     ASPAALILNMNDLAIDAFKALETLQADPALRSLPILAFANHEETDTWRRAKELGVTKI
                     VSRNEFSTRTRELVEDIIANSSQQSPVRSELNAEG"
     gene            24944..26035
                     /locus_tag="PK224_06100"
     CDS             24944..26035
                     /locus_tag="PK224_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HRB15342.1"
                     /translation="MKQSRLHDQHQQLGAIFEDTAGWSMPAHYGDPAAEYAAVRGAVG
                     LSDLSHRGKIRVTGDDRIKWLQSIISNDILPLQPGQGRYSSFLTHKGKMLGYFRVYVQ
                     ADAVWVEDVGEVGEATFQALRKFLLYGTKAKMENCGESWGLLLVSGPKSAEAVAAAFG
                     IEVRALQLLHTLPATIGGQQALILRTEETGEQDFEVLLPADAVPAAWNQLMTSGAPFG
                     IKPVGAQARELLRIEAGLPKAGPDLNEEIVPPEANLEGKAFSLSKGCYPGQEVVARMD
                     TYGNVRRHLVGLIIQDKTVPPAGSKLFSGDREVGWVSSAVFSPQRNAALAFGFPLRDF
                     SAPDTTLTVEVAGTRHQATVHALPFQKHS"
     gene            complement(26215..26697)
                     /locus_tag="PK224_06105"
     CDS             complement(26215..26697)
                     /locus_tag="PK224_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15343.1"
                     /translation="MAGLLSADEIFEKAKDAAVGATNADEKALQIDYEALQLKFQAAL
                     GDRKVARCHINKFLPEGYEDQGRFNLVLLTAGNVIFDIVIGDSYFRYDVVSVGQLDKV
                     QVIDAMWDNREKRREEPFLSLRLMHAEETHLLLALDDDERKSLLGFASAVSAVRNPEK
                     "
     gene            complement(26752..27006)
                     /locus_tag="PK224_06110"
     CDS             complement(26752..27006)
                     /locus_tag="PK224_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15344.1"
                     /translation="MAMQVISVIGALMVLVAYGLIQAGTWRELDAGYLALNILGSLLL
                     GIVAIEDRRVGFVLLEFAWAGIALVGVARAIRARRAARSL"
     gene            27259..27495
                     /locus_tag="PK224_06115"
     CDS             27259..27495
                     /locus_tag="PK224_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15345.1"
                     /translation="MSGQSCLIDGCQARVYAPAGTHAICKDHFLGFLTWRRRRGPQMF
                     RKYAGMTMDERDLIAGEWLQTLGTKDLPHPIPGL"
     gene            complement(27579..27935)
                     /locus_tag="PK224_06120"
     CDS             complement(27579..27935)
                     /locus_tag="PK224_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB15346.1"
                     /translation="MLAEKKDSRVGNHEVTEDGVFTTHRPSSDALAPAAVKRRPEDLT
                     PRELEILRLIWDGHTNRSLAEQLNISMKTADTHRSNMMKKLRACNTAQLLKAALEGNL
                     LETDPGLRRPGRGGQR"
     gene            complement(27995..28240)
                     /locus_tag="PK224_06125"
     CDS             complement(27995..28240)
                     /locus_tag="PK224_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15347.1"
                     /translation="MMVKGFLLKDREEQEYLLAIQPATHGMFERLIVRQKIDGSWVEL
                     NGNAQGSWQPRTQAITAVLGYLQRLWDTSKPALARGN"
     gene            complement(28659..29066)
                     /locus_tag="PK224_06130"
     CDS             complement(28659..29066)
                     /locus_tag="PK224_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15348.1"
                     /translation="MSGEARMTVGAGWLALGLFFLSGCTTVAQVTTWSDESCRQKVKD
                     QLQSILTDEGEQGDIANRLAVNTTVVLATGSLGPRPFGVSSPSGTDYSFFVQRKGEGC
                     LLRLFGRQKGFTRYTNNLTYISTRSLDGCACAE"
     gene            complement(29090..29482)
                     /locus_tag="PK224_06135"
     CDS             complement(29090..29482)
                     /locus_tag="PK224_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRB15349.1"
                     /translation="MVEQRIEEITRANLAFYAAFESLDMLQMDKVWAHLEYVTCIHPG
                     WSLRSDWPAVRDSWVLIFNNTFSMKFELSDVQVQVAGDLGWVICTEHLTSRQDDQPVE
                     TRVLATNLFERIGDEWLMIHHHGSPVMG"
     gene            complement(29632..29952)
                     /locus_tag="PK224_06140"
     CDS             complement(29632..29952)
                     /locus_tag="PK224_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB15350.1"
                     /translation="MWSEQQNVVQIEREANAQASTPGIGQALGGGLATKRRPEDLTPR
                     EQEILQLVWAGLTNRTIAERLHISIKTAEAHRANMMKKLRVSNIAQLLKTALEEGLLA
                     THAG"
     gene            complement(29971..30237)
                     /locus_tag="PK224_06145"
     CDS             complement(29971..30237)
                     /locus_tag="PK224_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15351.1"
                     /translation="MDVAGFLLSHDADHDVLVTIPPSSLGPFDQPDVKEQIDGYWIDM
                     SRVPHRRWTDRTRAIGAVLTYLQGLSPILSGFPTATREGMKGLN"
     gene            complement(30620..32014)
                     /locus_tag="PK224_06150"
     CDS             complement(30620..32014)
                     /locus_tag="PK224_06150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB15352.1"
                     /translation="MRVLLVEDHIDIRRLFEQVIEARGHDVTACADGESAWEAYSRRP
                     YELVLLDWELRGGGMDGLQVCRTIRSSPGGDRCVIVMITGHDSPESLRTALQAGVNDY
                     LVKPVGVEFLKLRLTIAEQWVESVRRRFAAEDQAQALQSQLADHGKFHDLIGRSPAMV
                     VLYEEIQKIAAVDATVLIEGETGTGKELVARAIHLSSRRASHTFLAVNCAGFTDSILG
                     SQLFGHKKGAFTGAIDNQEGVFEAAQGGTIFLDEIGDISPAVQTSLLRVLQEREVTRL
                     GESKPRKVDVRVVVATHHNLAEDVEKGTFRRDLLYRIKVARLQLAPLRERPTDIPLLV
                     HAFLGQFRSVMEKAVLQVSPEALQVLINHSWPGNVRELKSAVESALIHCKGTVVRVED
                     LPPEVRNPEPAATYVHFKRQDERTRMVGALQQTGGNRSEAARLLGMSRRTFYRRLAEY
                     DVSALGDGGDAPLQ"
     gene            complement(32029..36051)
                     /locus_tag="PK224_06155"
     CDS             complement(32029..36051)
                     /locus_tag="PK224_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15353.1"
                     /translation="MLAAIYLVTAGVFALDVAIPLGHGIWLLYLFPLWLSSRLILPAA
                     PLRFASVCTVLLAGGLWLAPPGVDVTTVMFNRALGTVMIWGAAYLLSQRREAEAALRS
                     ATSRLELRVAEQTKNLVSANGRLTAQLAEQRRIEAELRESRERFELAAEGADVGVWEW
                     DLQEHTAYYSPRWKSQLGCAESEIGATVADWEARLHPDDRARALATVTLFQEGYTKQY
                     RLDHRLRHHDGSYRWISSLGAGVCDEQGRMIRMTGIHLDVTARTQAEEATRESEDRYR
                     ALVEQAGDIIYRTDAAGRFTYCNPTSLRVLGYLPEELLGRHYLEIVLPQCRPQAERFY
                     GRQFIRKTPRTVYEVPVATKDGREVWLGQHTQLLLEGGVVSGFQVVARDITERKGVEQ
                     ALRESEERFSKAFHSSPAGMSISRLEDGRVLDVNEAFVRVSGFSREELIGMSSLDIGI
                     WVNPEDRRHLAEILRRDGFLRHLEKEFRTKSGEVRHGLFNVEPVCIGRETCVLTLVLD
                     ITRRTEAEAALRRSQAVLQAHQHALMRLTKSQHIGSGDWQSALEELMQTSALVLAVDR
                     ASVWLLDRTGTILDCVELYEQGQSRHSRGQRLNVVEYPRYFAELLQEQVVDAHDVAAD
                     PRTSELVQPYLGALGIASLLDVPIFFGGRLAGVVCHERVGVPREWTREEVQFATSVGN
                     LVTLAYEAKQRLEAENALMTAKEAAEFANRAKSDFLATMSHEIRTPMNAIVGMADLLS
                     ETPLNEDQREYVQIFRDAGSNLLSLINDVLDLSKIEAGHLDLDLVDFDLNDLVQRAAE
                     LVAVRASEKNLELAYQIQPDVPTSLVGDPNRLRQVLLNLLGNAIKFTDEGEVVLRVER
                     DPQADGPGTILFTVRDTGIGISTDKLTAIFERFTQVDSSTTRPYSGTGLGLTISRRLV
                     ERMGGKMWVDSELGRGSTFSFTAKFAVHVQPAPAVPPAQWEQLASLRTLIVDDNATNR
                     LIVRETLAGWGIPAAEVPGGEEALFELRRASKAGVPYRLVILDVRMPKLSGWQVAETV
                     ARTPGLAGVSIIMLTSERRAGDQARARECGVVRYLTKPFRRSDLFNGMMAVIGKVALA
                     EGNALLSQPPDSTGDMPGLKVLLAEDFVDNRRMMEFYFKSTPHRVETAANGQLAVEMF
                     QRGSYDLVLMDIQMPVLDGYAATRAIRSWEQAQGRSPVPILALTANALQSEVQRSLEA
                     GCTTHLTKPIRKARLLEAIQLYFQTSVPVAPSSSDASADTVVLQVSGEFESLMPGFLE
                     HRRQELIQIADALDRGDFETIREIGHGLKGAGGTYGLEAISAYGRALETAAGQKDGVC
                     VREALHRLTRFLERLQLVYV"
     gene            complement(36362..37207)
                     /locus_tag="PK224_06160"
     CDS             complement(36362..37207)
                     /locus_tag="PK224_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HRB15354.1"
                     /translation="MMISQDGVLDRGSVAVVVPAYNRAQFTPDEEISFRHLDHYLGRY
                     DKFLVVPRSLEIERPGYEIQRFDDCYFGSAIANGKLMLSPAFYESFRKYRYILIYQLD
                     ALVFSDQLLEWCATDVDYIGAPWVQCADSPWVGTPRVGNGGLSLRKVSSFLRVLSSEQ
                     YWIHPDVYWQRVVSGTPWYLRGVYLPRKWFKYIKRFNGVKRQVAQWHLRPDGTKNEDH
                     FWSDEAVRYDDQFKVASFDMGLRFAFEVVPRHCFELNNRRLPFGCHAWPRYDRAFWEP
                     YLLKS"
     gene            complement(37577..38677)
                     /locus_tag="PK224_06165"
     CDS             complement(37577..38677)
                     /locus_tag="PK224_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB15355.1"
                     /translation="MTKLRVGVIGAGAFAESCHVPGLQSHPQAEVVVLCGRDSARTFS
                     MAQRLGVPEVSLDYEEVCARPDIDAVTIATPNVVHARQAQAAFAAGKHVFCEKPLGMN
                     TEEAADMLRAAEQSGKIHQTAFTYRYLYGVQELKRRLLNGEIGEPYYVAVHYDSWDGL
                     RPGSRIGFREHLDSAGAGVLYDVGSHLFDLVGFVLGPIEAVSGNTILVPRKRIDARTG
                     ALAHVETDDIASAAFTCRGGIQGQLFASRATPNSGDKAYIELVGRQGALKASLSRGSV
                     DVLTISRPSQPGWEVVPLPVQASDGQPHCLSRMMHSFVEACLRGSLNGEIDASFHDGL
                     AVQRAMDAVRQSSAEPAAISLKAAFDPSPDYS"
     gene            complement(38926..39840)
                     /locus_tag="PK224_06170"
     CDS             complement(38926..39840)
                     /locus_tag="PK224_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRB15356.1"
                     /translation="MQKPSVFAAYAALTTSALVWGGSIVGQKLALGAFSAVETSLLRG
                     FGALVILIPLWWWSEGGRTTWTARDLRLLSLLGLGVLGNHLLTLFGLRYIGAATAGVI
                     IGASPAITALLSSLLVRDIPFNTVAAGCAVSFAGVALVSGVGGEAPSGDNPWLGGTLV
                     LLGLVSWALYSIGGRQVMERLSPLTVNWTTLLLSLLLQIPLLWTDQKLLLTGASVVPV
                     SGWVALGYLIVFATALGQQAWLYGVQGVGPSRAGVFVNLIPVSALLLSALVLGEAIGV
                     REIVGIALILAGVWLVGWQSARLKHAGG"
     gene            complement(39900..40550)
                     /locus_tag="PK224_06175"
     CDS             complement(39900..40550)
                     /locus_tag="PK224_06175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB15357.1"
                     /translation="MTDLVLPKIDAYAAAYSLPETPVRRALREETERTMEFARMLVGP
                     LEGAFLHMMTRLVQATRVLEIGMFTGYSALCFAEALPEQGQVTTCEVDDASAAVARRF
                     FAQSPHGRKVEIRMGPALETMAGLTGPFDLIFIDADKLNYVNYYRRAMELLSPRGVIL
                     IDNVLWDGDVLLEPPPDDRTAAIQELNRVVRTDQRVSAVLATIRDGIWIITPTPFA"
     gene            40637..42157
                     /locus_tag="PK224_06180"
     CDS             40637..42157
                     /locus_tag="PK224_06180"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRB15358.1"
                     /translation="MKTLATLEPLTTRLLEIRRIQSAAAVLSWDQETYMPAGGGAARA
                     EQIATLEGLAHQRLVSAELETLLTEWIDPATGQAADSWDEPSRSLLRETWRDFSRAKK
                     LPSDFVIRLSRECSLAQQAWVTAREESRFSKFLPSLKIILDLKRDEARYLGYRNSPYD
                     ALLDTYEPGATIAQLAPLFTQLRERLVPLLRRVQASHVTIDDRCLHQSFDQSKQVEFG
                     RLVLTAMGYDFERGRLDLSAHPFTTSFHPTDVRVTTRVFEKDLPSCLFSCIHEGGHGL
                     YDQGLDPRYYGSPLGESVSLGFHESQSRLWENCVGRSRAFWHCFYPMLQHTFPQQLAD
                     VPLDRFYAAINRATPSLIRVEADELTYNLHIMLRVEIEQALIEGRAEPDDLPGLWNEK
                     MQSYLGIVPERDAEGVLQDVHWSMGAFGYFPTYTLGNLYSVQFFEQATLELPQLEEEM
                     RAGHLLPLRRWLEQKIHRWGRMFTPDHLARRVTGSGVNPEPFLRYLETKYAELYRL"
     gene            42646..43350
                     /locus_tag="PK224_06185"
     CDS             42646..43350
                     /locus_tag="PK224_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB15359.1"
                     /translation="MSGTLAVDACQDSHPAKAEVRILLADDHALLRRGLREFLRDFLV
                     EDGLTPDIVETASARHAIDHIRTGTWDLVILDLNLPDMPGLEVLRILKSLRPALPVLV
                     VSIYAEEHYSARAIRAGALGYLTKERGPQELRMAVSRILQGGHYLPPPETEHNQNEGP
                     HRNLSSTDTLPATLSDREIEVLQWIAQGRRLTEIAEHLNLSIKTVSTYRSRLLIKLGM
                     RTTAELIRYALDQQLV"
     gene            43481..43867
                     /locus_tag="PK224_06190"
     CDS             43481..43867
                     /locus_tag="PK224_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15360.1"
                     /translation="MANILIINADANLKRLIKLALPGMEHSVQDSATLQTACAQSTSS
                     KLDLVISRFVADDDQGPKDLMELRKTFPTARLIVTAALHDPALEGEKFQRVVRELHIE
                     YCLLEPIETGAMLHTIQSALALPKRP"
     gene            complement(43955..44518)
                     /locus_tag="PK224_06195"
     CDS             complement(43955..44518)
                     /locus_tag="PK224_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15361.1"
                     /translation="MRKSHEMTIDRVLLLYALSLAEPYGLLSDVKLQQLCFLCELQMF
                     GKGMKGFHFEFVRFAYGAFSRDLDNDLTSLRRKERVENFSPSDQVRDEVLPLLMKGID
                     GNETNEKIRDLMQAVITTYGPQDVTAITQSVESVELSTPQQPDFKIPIRDIVFHTTLL
                     VPARIEASAEFLLPPSQLGKLNTAMGY"
     gene            44672..45103
                     /locus_tag="PK224_06200"
     CDS             44672..45103
                     /locus_tag="PK224_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRB15362.1"
                     /translation="MSKTLKEMDVLRQHLAKHQLKLTRQRELILTAFLRQEHVTAETM
                     YHQLAKKDPHLGLATIYRTLNLFCEAGIAQARHFGTQTQYDNISHKGHHDHLICTGCG
                     TIVEFENCEIERLQEEVATKNGFVIQTHRLELYGLCARCRH"
     gene            45199..46230
                     /locus_tag="PK224_06205"
     CDS             45199..46230
                     /locus_tag="PK224_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRB15363.1"
                     /translation="MASSCRTFLALTLLLGALTLSQWATGTAEAAEKLVVYSGRAERL
                     IKPVLDEFQAKSGIQIELLSSGTTELVNRLQAEGDHTPADVFLTNDAGSLEHARELKL
                     LRPMNMREVERAIPSQFRAADNSWIGLSGRFWIVVYNTNLVKPDQIKSLFDLAQPQWK
                     DKIAVPNSGSEYLQAGVSVIKATFGDERTKQFLQGLKANAGTQVYQKSSQIVDAVAKG
                     QVAAGIVNHYYIYRHLATQPTAPIAAVMTDQQEGGMGAIMNVTGIGVTRASKHVESAK
                     LLIEFLVAQAGQKMFADLDKEYPLHPDVKADPTLIDRRTFRAAQVPLARLAELREATL
                     TLIEQVGLR"
     gene            46249..47853
                     /locus_tag="PK224_06210"
     CDS             46249..47853
                     /locus_tag="PK224_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRB15364.1"
                     /translation="MLTATRAHAPSSLQWISLITAAFLVLPTCYIIYVALTAAPTVWS
                     RLWSTRIPELLWNTLSLATGVALTTLLLGVSLAWITVRYEFPGRRVWEWALALPLAMP
                     TYVLAYVYAHLLGMGGPVEQGWQTLMGSDARLLSPQSFVGVTLIMALDTFPFVYLLVR
                     GALLNLNISFEEVARVCGVSPWSTLWRVTLPLIRPAIAAGLALVILYVISDFGAVSLL
                     RYQTLTYAVYQQMTSRYDHTSASILSLLLVVMAIIFLVTERWFRQRSRFYQTSGRYRR
                     ATRHHAGPAGTALITGYVVLVFGAAFAIPAVMLIQWSIEAIAQGALDARFFGFIWNSS
                     FLSALAACGAVIIGLPLAYLASRRPSRLNLACLQAAYAGYALPGPVAALAVLVLFTHF
                     VPVLYGTVAVLLVAYVLHFLPVGLQSMEPALQQVTPNVEEVARTLGCTTRRTLRRVTL
                     PLIRNGFIAAWVLMFLQTMKELPATLLLRPVGFDTLAIRVWLEASEEYYQLAAPAALL
                     IVLLSLPALLLLVSKDWRGRDQEVVS"
     gene            47907..49028
                     /locus_tag="PK224_06215"
     CDS             47907..49028
                     /locus_tag="PK224_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB15365.1"
                     /translation="MPDAMDLADEQSPVLELRGISCAYEPNRPAVEQITLTVHQGEIL
                     CLLGPSGCGKTTILRAIAGFERVTDGSISLSGRLVSSRDVMVPTEQRHIGMVFQEYAL
                     FPHLRVEKNIAFGLSHLSRPQQKAIVDDLLTLTGLRGLEQRYPHELSGGQQQRVALAR
                     ALALRPVLLLLDEPFSNLDPDMASRMRQDLHALLRQTKTTAILVTHDHDEAFSMADRV
                     AVLNRGRLEQFDTPEAIYHVPTTPFVADFVGQADFISGVVANDLVTTEIGEFPNTQHL
                     ATGTSVVVMIRPDDIHIVPTKGADAHILARQFKGSENVYTIQLPSGQIVHSSESSLSI
                     YQVGTAIALRVVATHTVLFPQPPESSGKNEPGHGNAPPP"
     gene            49115..49546
                     /gene="exbB"
                     /locus_tag="PK224_06220"
     CDS             49115..49546
                     /gene="exbB"
                     /locus_tag="PK224_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HRB15366.1"
                     /translation="MDLLKNAVEYGIIGMLIALSIWSVAVAVERWLYYRRVDLSQFTD
                     IQTFEMALTKRLVIIGTVAANAPYIGLLGTVLGIMMTFHTMGTSGTMAVNTIMIGLSL
                     ALKATAVGLLVAIPCVVMNNILRRRVTELLTIYKVQHGTRG"
     gene            49530..49904
                     /locus_tag="PK224_06225"
     CDS             49530..49904
                     /locus_tag="PK224_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRB15367.1"
                     /translation="MEREVNQINVIPLVDVMLVLLVIVLTTATFISTGQIPVNLAKAK
                     EAGDHKDVPVVVTLTANGDLFLNDRPVPPDGLKTVMLAHPRESLVVVRADKVTLLERF
                     VSVVDEIRGLGFQSVSLEVVRL"
     gene            49901..50863
                     /locus_tag="PK224_06230"
     CDS             49901..50863
                     /locus_tag="PK224_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRB15368.1"
                     /translation="MTSRQPGISDPFIRFHASGWIISLCLHGSAILLAGLLVAKIGLA
                     PPSSSFQWDVTVVGSQPPATPAQVAPGAQPPATAVIRPAQRNAPAAALRPTPSQAALP
                     TIPATTSSSSATVERNTIEAALTPPHQAPAQLLERAPVVPPPPELQEAKDELQTSLHT
                     TSAPVEAPADPRSLPQMANPPLADAPLAPEMSGPTSQPPSELATSPAQTASLVPSNST
                     TQALRKPDYGWLAGPLLQRIEALKQYPATARLHRLEGRVIVRIVIQQDGYITSATIAR
                     SSGHDVLDQAALETIRQASPLTLSQPLEKSSVTMQIPLGYYLDR"
     gene            complement(50873..51244)
                     /locus_tag="PK224_06235"
     CDS             complement(50873..51244)
                     /locus_tag="PK224_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15369.1"
                     /translation="MMASSNSIQGGRSGWGLAPGRRWEGMRAGIVVGLLVLSACSGSS
                     KYFHDAVNEATQEMVGKRYGSPHKTQAATGGGEIWTYFERGSGTAGFGGQVRGGGCRA
                     YVLRFDKQAVLRGWEQQPCQG"
     gene            complement(51228..52253)
                     /locus_tag="PK224_06240"
     CDS             complement(51228..52253)
                     /locus_tag="PK224_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRB15370.1"
                     /translation="MGYRFAVMSGIFLLLFSSGCATTRSESAPVVVKRTQMQMGTLVT
                     ITSVASDRRRAQDAASAGFQEIHRLEELLSTWIATSELSQVNAAAGRDAVMLSRDTMK
                     VLEASLEMARLTEGGFNILVGPAVEAWSVLDRQHIPSDAVLQAVRPLTELRSLHLNVA
                     ERTVYLAKPGMRVDVGGIGKGFAADMAVAAMQAAGATAGVVALSGDIRTFGRLPGGMK
                     FPFGVRHPRHEEAVLAFIELQDEAISTAGDYERYFERDGVRYHHILDPITLQPARDCQ
                     SVTVVARDGLTADGLDTGIFVMGRERGLALVERLPGVGAVIVDRDGKVWVSSYLKGRV
                     RMNDGIE"
     gene            52904..54352
                     /locus_tag="PK224_06245"
     CDS             52904..54352
                     /locus_tag="PK224_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15371.1"
                     /translation="MKIRAILGALVLASLPAMPALAENMTPEDIKKLVDEAVEKRLQE
                     HERREGGMQQAQGEAASTPQYPVASGPITEIRVERKGEEKIPLGFGSTGSGKLIYAKP
                     FLSAPKATVGGYADVMYNSLSRQNLDNPSRNSFGQQRLVPFIYADITDHIKFATEIEI
                     ERGGSNAPQGTDGSMQIEFAQLDYLINEAINLRAGILLMPVGKFNLLHDSPLNDLVDR
                     PMVSRIVIPSTWFEAGAGIYGTLYPSSRSKLDYEVYAVNGMSQTAGGITDLGVRSARG
                     SVSRDRDDNKAVVGRIAFSPMLGIEIAGSGYHGAYKPAAGAVGAGYIDMFALDWTLQK
                     GPFEIIGESAWTRISNNNATGVANRVIGPAGMQGYYVQGNYHFMPEFLKKWAPSHFTD
                     ASTFTAVVRWEQVNTDTDDRTKQNALGGQRKLERLTLGLNFRPIEDTVIKFDWQFNTQ
                     RDAKGLVAAGDLGTAGSPMNGNGFLIQAATYF"
     gene            54413..54874
                     /locus_tag="PK224_06250"
     CDS             54413..54874
                     /locus_tag="PK224_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15372.1"
                     /translation="MTQHYRRLSVLLIPALLTACASLGGMQERFAVCSYDHAWEAALD
                     AVKDRAIARQDKAAGLIDTAWLEIPMPGRTFGALQRDLGDSKDRSRISLAVKRLDDVT
                     KIGFIEERQRWAFRGGSRLFGWTDTESSAQVMTDVQNRLDTKLKEHGCSVH"
     gene            54859..55545
                     /locus_tag="PK224_06255"
     CDS             54859..55545
                     /locus_tag="PK224_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRB15373.1"
                     /translation="MFSTLSAYRTLTAVVLLLTSSLPALATEKVWDNELRRFLKDDDF
                     KYEEFMTEEEAVKTILPKSQRVRKELIRLTQDRKELIEQRIGWKFPEDSFDLYIGETG
                     DKVDGYAMIHNTIGKYKPMTYMVGVDPKGNCTDVELLVFRDAKGSEVGKKRFNSQYDG
                     KTVTDPIRINKDIINISGATMSVRSMNAGVKRVLVLVDEFYLKPAGLGSDTVAVQKAE
                     RGLLGTLFGN"
     gene            55569..56087
                     /locus_tag="PK224_06260"
     CDS             55569..56087
                     /locus_tag="PK224_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15374.1"
                     /translation="MRNFNARFRLRDSDRHIRLLYTQFLFLMLVGFLFSFFWAHSMTS
                     LSPQGIADHYRGSDATFGEPMSFRELAEVTHFHLFTMPVVFMILVHVLYLTQASHATQ
                     VWLTWLSFGGVALDLLSPWLISYISPIFVLTMLAGDTLMMGSFLAMMAIPLYEMWILK
                     QPLMAGKRGEES"
     gene            56192..56947
                     /locus_tag="PK224_06265"
     CDS             56192..56947
                     /locus_tag="PK224_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRB15375.1"
                     /translation="MHPSISPINATTQLIQEITRQLDALDSLQLSKESLHAVRLLQLR
                     RLTRRIETLIPGHFGHGERTAHYARLLGKAISLTDDQHIELHYAALLHDIGLLTLPGR
                     LLDETAAHSLDDYALIQSHPREGAALLSPYRFLSEAARLIAHHHERWDGAGYPYGLRG
                     GYIPLAARILAIADVFDSIANRSTSLHRALRTLQASAGSQFDPILTATFCARVRDQDQ
                     LPPLPFGDASVTALTVEPTLDQPSHQHMPFSMF"
     gene            57010..57573
                     /locus_tag="PK224_06270"
     CDS             57010..57573
                     /locus_tag="PK224_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRB15376.1"
                     /translation="MMMNNAPDIEIPTDRLRTLSSELRAQSPHWADRLEQATTEQELR
                     NLVCDLFNLSHALPSHRESANETTTLAGRITRFINENLHRGLTLKVLANFLGYSEKYC
                     SDLFYRIMGESFSGYMRRHRVERAQSLLTTTAQTLAEVATAVGFSDQFAFSHFFKRAT
                     GHSPREIRSRQAHSRHRLTVHAMQKAL"
     gene            complement(57826..58305)
                     /gene="bfr"
                     /locus_tag="PK224_06275"
     CDS             complement(57826..58305)
                     /gene="bfr"
                     /locus_tag="PK224_06275"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRB15377.1"
                     /translation="MKAKEGVLDILNKVLVSELTAVHQYLLHAALCKHWGYGRLHEHF
                     NHLAQEEVSHSSGLIDHILYLGGAPEMDHLDAVAAGKKVSDLLEADLAFERDDADVLR
                     DGIAHCTKVADFTTRRRLEEMIVDTEEHIDWFETQLCTIRQVGLDRYLAEQIHSEKS"
     gene            complement(58341..58817)
                     /gene="bfr"
                     /locus_tag="PK224_06280"
     CDS             complement(58341..58817)
                     /gene="bfr"
                     /locus_tag="PK224_06280"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRB15378.1"
                     /translation="MKAKQGVIEILNKVLTADLTAINQYFVHAKMCSNWGYERLHHKV
                     RERSIDEMKDADELISHILYLEGVPNVQRMNTVHVGETVPEQLKLDLKAEQEMLTLLS
                     DGVVHCTKVGDFTTRHMFEDMAKDVDEHIDWIETQIETIKQVGLENYLAEQIHKES"
     gene            complement(59138..61477)
                     /gene="topA"
                     /locus_tag="PK224_06285"
     CDS             complement(59138..61477)
                     /gene="topA"
                     /locus_tag="PK224_06285"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRB15379.1"
                     /translation="MAKSLIIVESPTKARTITKYLGRGYSVMASVGHVKDLPTSKLGV
                     DLENDFEPQYVTIKGKSKVLADIKKKAQEVDTVFLAPDPDREGEAIAWHIAQELHGKG
                     KKKDGKVYRVLFNEITESAIKRALKSPGEIDMKLVQAQQARRVLDRIVGYQGSQLLWK
                     KVRRGLSMGRVQSVAVRLICDREKEREAFRAEEYWSIVALLAGDNPPAFEAKLHSVNG
                     QDADIGTGERAQQILDAVQGKPFVVQSIERKEKKRNPVAPFITSRLQQEAARKLHFSP
                     KKTMTLAQQLYEGIEIGAEGPTGLITYMRTDSPRISQEATEDARVVIRERFGVEYLPA
                     TPNVYKTQKAAQEAHEAIRPTSAARDPESIRQYLEQDQYNLYKLIWNRFIASQMVPAI
                     MDVTRVDSSPVGTPDRYVFRTTGTVVKFAGHTAVYLEGTDKELPSHKPKNEQEAEDES
                     DRQLPALREGESLHLVAQEAQTLPGLVSKQHFTQPPPRYNEALLIRELEEKGIGRPST
                     YASIISTIQDRKYVEKVEGRFLPTETGRTVNDFLLKGFPDLLDTEFTSHMEEQLDEVE
                     EGTKPWVSAVRDFYTPFVREMELAQGIPGPKDIVEPPTNLPCEKCGRMLEIKWGRNGK
                     FLACPAYKDDPPCKNTQNFEKLPDGTIKIVPKLEETTDEKCEKCSSPMVVKSGRFGKF
                     LACSAYPECKTTKAIALGVKCPQPGCGGDLVQKRTKKGRNFYSCSRYPQCEYALWDRP
                     INKPCPKCQAPFLIEKVSKQAGRSVQCRNEEECGYREAG"
     gene            complement(61560..62696)
                     /gene="dprA"
                     /locus_tag="PK224_06290"
     CDS             complement(61560..62696)
                     /gene="dprA"
                     /locus_tag="PK224_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRB15380.1"
                     /translation="MSHRSLRPWLVLRAIPGVGDAILLKLVHAFGAPDAVLSAPQSAL
                     EEIGCRPPLVEAIRRGPAPDAIRELDKELEQLQRRKVTVLTYLDAQYPAPLRTIPDPP
                     PLLYVQGSLLETDRHAVAIVGTRKVSAAGRIVAEELARDLVKMGFTIVSGLARGVDAA
                     AHRGALAGKGRTLAVMGCGLDRTYPADHRQLRETIEQEGAVLSELPLGAAPHSYHFPR
                     RNRIISGLSLGVVVTEAAIESGSLITARLAGEQGREVFAVPGFVKAEQSRGPNSLIKD
                     GARLVESAQDILEELLPQLDPAFRERLAGGAGTVAQPKVQFSPDETLVYDVLSVLPQS
                     VDDVIRRSGLPAAQVAAILLSLELNNSIRQLPGNEYVRVTSGMH"
     gene            complement(62816..64327)
                     /locus_tag="PK224_06295"
     CDS             complement(62816..64327)
                     /locus_tag="PK224_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRB15381.1"
                     /translation="MGVEIAISIAREETRVAVLDNGVVTDLYGDRAKDQDFVGNIYKG
                     RVAKVLPGMQAAFIDIGLEKAAFMHVSDLSMDVEPGDTLVEGDDDDNKDSEVPKPKRQ
                     SSKPIEELLSEGQEVMVQISKGPIGTKGSRVTTYVSLPGRYLVFMPTVEHIGVSRRIP
                     RDEERARLKEIMKRARRPGFGYIVRTVSEGVKEEELRSDVEFLHVLWQDVLTKRDQQP
                     APSLLHSDLSLSFRVVRDLFGRRVDRLLIDSREEYNAIKGFVQRFSPEQTSRIHFYDK
                     EESLFDYLGVEQEIARAMSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETI
                     LRNNLEAAREIAYQVKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMSSDKARTRIS
                     RVSDLGLIEISRERVREDLLRSLSEPCHYCDGRGYTKSPMSVAYEIFREVRRLGHEIE
                     QQRVVVGAHPAVALLLQEQEQPGVEELERRYSAKILVTPDDRLHLEQFDLVVI"
     gene            complement(64473..65591)
                     /gene="rodA"
                     /locus_tag="PK224_06300"
     CDS             complement(64473..65591)
                     /gene="rodA"
                     /locus_tag="PK224_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRB15382.1"
                     /translation="MIDRVIDSRGLDSFDLRFMGLIAVILSVGVLSIYSVTHSQDTAF
                     PFYLKQLVWILLGTIAFLVMYLSDYHKIARLAYPTYAVILIMLAVVLVMGKSSRGAQR
                     WIPIGPFAFQPSEFAKLVLVLVLANYYSRVSRAGWLHRVVLPGLIVLPGLLLILKQPD
                     LGSGLSFLAVYAAMLLMVGVRSKTLGVILLLSVMLFPFVWEMVWASLHDYQRERVMAF
                     VDPEYDPGGKGYHALQSRIAIGSGELSGKGLYGGTQSQLKFLPEGHTDFVFAVYAEEW
                     GFVGVLVLLALFIALIWVSLEIAARAKDTLGALLAAGIVAMLCFCVVVNIGMTAGMFP
                     IVGIPLPLVSYGGSATIMTMASLGLLLNVKRRRLTLFY"
     gene            complement(65591..67462)
                     /gene="mrdA"
                     /locus_tag="PK224_06305"
     CDS             complement(65591..67462)
                     /gene="mrdA"
                     /locus_tag="PK224_06305"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRB15383.1"
                     /translation="MASIGFNDSDLLDLQRRLVILRVGLLLVVALLALRLWHLQIREG
                     PYYRDLSENNRTRSVVLEPARGLIFDRNGVLLANNVPSFALYVTLEDVKDRPALVAQL
                     ASLLNLEPEVIQKKLSTGKGSKLQPRKVKDRLTLREATLIESHRLDLPGVMIQVESQR
                     NYPGGPVAAHLLGYVGEVSADQLEKADFADLHQGSVVGQYGVEKWFDRQVRGRAGQKS
                     VEVDALGHEKRAVVSDKPVAGDDLYLTIDARLQKTAEDLLGEESGAIVALDPTNGDIL
                     AMASRPGFDPNMLSKELTNKQWVEIVQNERRPLNNRATQGQYPPGSTFKVVMAAAALE
                     SNTVTPSTMVRCNGGYQFGNRLFHDWKQGGHGSVDMNEALIHSCDVYFYTVGQRMGID
                     TIAEYAKQFGLGQETGVELPSERVGIVPSTAWKQKARNQPWYPGETISAAIGQGYVTV
                     TPLQMASVIGTVANDGLSIKPRLVQAVMNRATGERAEFPPTVRGKVAVKPATIALIKA
                     ALADVVTKGTATRAKSSLVTIGGKTGTAQTAALRTGPEKDIPKRLRDHAWFVAFAPVE
                     APRIAVAVLAEHMGHGGSAAAPLAKDVIEAYVKAYPEVLNGVPAGGRTKSADARPKT"
     gene            complement(67508..68008)
                     /locus_tag="PK224_06310"
     CDS             complement(67508..68008)
                     /locus_tag="PK224_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15384.1"
                     /translation="MKFLLYLLLALVVVPFQTTLLHYFSIFGVRPDLGLVAACLVGFL
                     GGELDGLILGLLLGCFQDMLSAGDLWINVVTKGGGGFLAGLAGRHMAHITPAVLTAGL
                     AIISCLSSAVFLYSMNPAGIDEVWMGVRSTVLVQAGFDALVGTGLYLLFRQRWSDDRM
                     VTEGAL"
     gene            complement(68005..68784)
                     /gene="mreC"
                     /locus_tag="PK224_06315"
     CDS             complement(68005..68784)
                     /gene="mreC"
                     /locus_tag="PK224_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRB15385.1"
                     /translation="MLQYVGGPLGQILSVPLAAFSSVDHGISETWNAYLALQSVQEEN
                     RQLHRDLELLKGQNNQLRESVAATRRYETLLNFKQQSPSQTLAAQVIGRDATNWYGGM
                     ILNKGESDGVRVEMGVVTPAGVVGRIVKTNSTSSVVLLVTDPNNAIAGVVQRTRDEGI
                     VEGTSHGRARLKYIPLLSRVQAGDRVVTSGLTGTFPRGLAIGGLTQVEKSEGDLFQSA
                     EIEPEVDLSKLDEVLIITAPYEDADSAQKLLQEIRGDRKKP"
     gene            complement(68905..69945)
                     /locus_tag="PK224_06320"
     CDS             complement(68905..69945)
                     /locus_tag="PK224_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRB15386.1"
                     /translation="MSDMFGWFSNDLAIDLGTATTLVYVQGKGIVLNEPSVVAVEKKT
                     ERVMAVGADAKRMLGRTPGNILAVRPMKEGVIADFEKAEAMLSHFIQKAHNRTAFVRP
                     RIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMVVDI
                     GGGTTDIAVISLGGIVYSESVKVAGDRMDDAIMNYIKKKYNLLIGEHMAERIKFEIGS
                     AYPFEERKTMMIKGRDLISGIPRTLVVDDAEVREALQEPIGTIVNAIKVALENTPPEL
                     AGDIIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELDLLR
                     KVSVMSQCSTSR"
     gene            complement(70003..70548)
                     /locus_tag="PK224_06325"
     CDS             complement(70003..70548)
                     /locus_tag="PK224_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRB15387.1"
                     /translation="MNGARGKIATLFVQVKEICYSVEELERSPVVEEAIGHSPLELGV
                     YPKAQVLNRFIAKMIDLLIVAAVSKLVPPIGVLAGLAYLLLADGFGGGRSVGKRLIGL
                     QTIVPRTRDPAGFRESIIRNLPCGLAQLAFEIPYVGWIGWGAVLSLEGLLVIGNEQGR
                     RLGDEIAKTQVLESGQLDVSD"
     gene            70622..71320
                     /locus_tag="PK224_06330"
     CDS             70622..71320
                     /locus_tag="PK224_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRB15388.1"
                     /translation="MIKLLREAAHDYPWFLKSIMGLLALAFVITMGWWGFGQEGGNVV
                     ASVGDQVVPLDEYRRAYENTYRFYKDKGQNDIKDEFLKQFVLEQLIDNRMWLHVAKEM
                     GLTVSDEDLRKAIMQRTEFQKNGNFDPEAYRRLLSANRLTPASFEAMEAKDILTNKAR
                     LVIMDAVALTPAEYTEAQTLAAREGDADPAKAAAAKERVFQSLLFQKQQRALMAYAES
                     MKSKVPVKIHKELM"
     gene            complement(71313..72353)
                     /gene="queA"
                     /locus_tag="PK224_06335"
     CDS             complement(71313..72353)
                     /gene="queA"
                     /locus_tag="PK224_06335"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRB15389.1"
                     /translation="MLLSEFDFPFDPVLVADHPVIPRDRARLLVLDRQGGERVHRQVA
                     DLPSLLQSGDLVVVNNTKVMPARVPAQVRSNGKLLDLLFVEDLGQGLWEVLIKGRFRP
                     GAVIEFPGGGTGEIVERSRLRTTVQVSGVASVYELMQATGTMPLPPYIKREPSADDQE
                     WYQTMFAQHEGAIAAPTAGLHFTRELVQALAGKGIELAQVTLHVGPGTFRSVKTERIE
                     DHRMLAERIEVSAQTVAQIRLTRERGNRVVAVGTTVVRALETAARGGRGIEPFQGPAE
                     LFITPGFEFQVIDAMVTNFHLPRTTLLMLVSAFVGVQPLRAAYEEAVAQRYRFYSYGD
                     AMLIGSGWATVT"
     gene            complement(72353..73564)
                     /locus_tag="PK224_06340"
     CDS             complement(72353..73564)
                     /locus_tag="PK224_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRB15390.1"
                     /translation="MALSPLTRHQSSSLAAAGGMLLLALFCVPAAFAESIRVLLAQEA
                     PLVEVRSEGALALVTETGDTKILQAPIHLTARGDGVHVDGRRSMGGQVLIRPLRNNLS
                     LVIGKEGGATAGAPLALSGVVRLSRKGHGLSVVNQVDLEEYVKGVVPSEVSSAWHPEM
                     LKVQAVAARTYALYNKMLSAARDYDVVATIQDQVYRGRTGVDRRVEDAVESTRGIVVT
                     HQQAPIYAAFSSTAAGPTEDAVNVWANKDLPYLKGVECPFDLESPYYQWRASVKIDQL
                     EHNLRHQGFSVGTIATITPIAYSRAGRVSRLRILHSGGETVLRGEDLRKAVGYTVIPS
                     TQFEVESIGAEVVFAGYGAGHAVGLCQWGAKELAELGYSYSSILQYYYPGTELHNAAL
                     SQLMMPVIPTP"
     gene            complement(73546..73800)
                     /locus_tag="PK224_06345"
     CDS             complement(73546..73800)
                     /locus_tag="PK224_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRB15391.1"
                     /translation="MAAWTGIGRSLIVAGLVLVAVGLLLTLAEKWSGLGSAFAWIGKL
                     PGDFSVTRERFSLYVPIATSLVFSILLSLVFYFLSWLFRR"
     gene            complement(73804..74823)
                     /locus_tag="PK224_06350"
     CDS             complement(73804..74823)
                     /locus_tag="PK224_06350"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRB15392.1"
                     /translation="MLKKKQAYTVAILGATGAVGKESLEILEERNFPIETLRLFSSKR
                     SAGDVLSCQGKEYKVEELTEASSFAGVDIAFVSATDTISRDYGARLGAAGVVVIDDSA
                     IFRMDPDVPLVVPEVNAAALRSMKRGIVAIPNCTTTPLVMALKPLRDIAGIKRVVVTT
                     FQSVSGTGSAAMDELVDQTKALMAFQDVKVQVYPYQIAFNLLPQVGSFGDGGDCSEEV
                     KIVQETRKILEMPSLRVTATTVRVPVLRCHSEAINVELERPLKANDARAALAEMPGVI
                     VYDDPVKKLYPMPFDVSGKDDVYVGRVRVDESITNGLNLWVVSDNLRKGAALNAVQIA
                     ECLIQ"
     gene            complement(74834..76048)
                     /locus_tag="PK224_06355"
     CDS             complement(74834..76048)
                     /locus_tag="PK224_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRB15393.1"
                     /translation="MTTRTTQSGIIETLAGNGEPGYAGDGGPAGAASLNEPKGLCVDR
                     EGNLYIADSENHVVRRVDRVTGIITTVAGVCPSGATGPAVPEPPVAAQAEEDEDPFAD
                     HSGDSTKAYTQVTDLSGTVRYVTGGRLTVEHDSGDGGPARRARLNFPSAVAVDRAGNL
                     YIADTMNHRVRKVDAATGIITNVAGTGQARYSGDDGPAVSAAINEPTGLAVSDEALYI
                     ADQSNNRVRRVDLATGVITTVAGDGTAAYSGDQVSAVQASLAGPSGVALGGDGVVYVA
                     DTFNSRIRSVDPATGQIATVAGDGGTYRYQGPAEPSSPSLSRPAGIAVGSDGNVYMTD
                     SDSHLIRVWNGRSKTISRLSGTGVAQFGGDGGDALAGSLSYPFGVAVDASGTVYVADT
                     FNHRIRVLTATA"
     gene            complement(76052..77128)
                     /gene="leuB"
                     /locus_tag="PK224_06360"
     CDS             complement(76052..77128)
                     /gene="leuB"
                     /locus_tag="PK224_06360"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRB15394.1"
                     /translation="MNAKIAVLAGDGVGREIVPEAVKVLKVVAERYGHTFDFISGDVG
                     GHAIDKVGVPLPAETLAIAKHSDAVLLGAVGGPKWEGLEYSLRPERALLGLREQLGLY
                     ANLRPAKLYPMLADASTLRRDVIEGIDMLVIRELTGGIYFGKPKGIEKLPGGGERGFN
                     TEVYTTEEIRRIAVVAFDAARKRRKKVTSVDKANVLESSELWRRVVTDVHKGYPDVAL
                     SHIYVDNCAMQLVRNPRQFDVLLCNNIFGDILSDEAAMLTGSIGMLPSASVGAKVGLF
                     EPIHGSAPDIAGKNIANPIATIASASMMLSYAFQLDKEAAAIEQAIVKTLELGFRTKD
                     IHAEGMQLVGTVEMGDAIVRNLPS"
     gene            complement(77147..>77398)
                     /locus_tag="PK224_06365"
     CDS             complement(77147..>77398)
                     /locus_tag="PK224_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB15395.1"
                     /translation="AHGLLAPGQQSLWHRLKSSEVYYFIAGRGVMKVEDESVAVEAGS
                     VIYVPPGAKQSLVNNGTAPIEFLCLVDPAWTAEDEAVGG"
BASE COUNT        16708 a        24461 c        20820 g        15409 t
ORIGIN      
        1 tttgacgaaa acgacccgca gagtatggcc cggctcatga aaaaaatggg gcaggaaatg
       61 ggagaagacc tcggggatga cgtcgaagcg gcgatggaag gcgggggcgc tgctccaccc
      121 ggaatggacc agaccgactt cgagtgaccg ccccggtcat gaatattcac ttgacaggat
      181 tagccttgtc tcctaccatc agcgaagaaa tggttactcg gctgattcct atgcgcctac
      241 gtacagctcc atcgactctt cactgtcgcc gccatcacga gcgaggctgc cgataatgtt
      301 tgccggcgaa tacctctgca aagtcgacga caagggacgc ttcctgatcc cctcccccct
      361 tcgagaacgg ctcgaggccg agggcaatca ggtcatgttc gtgaagaata ccgagcagac
      421 actctggatg tactcagcca aggaatggga aaaggtcctg gaacggacca ggatcaccct
      481 ggatgaggac cagagccgcc tgttcatgca ccatgtgatg tcgcaagccg gcacctcgga
      541 tatcgataag gccggccgcg tgctgatccc gagccgtctt cgtaagctcg tcccgatgga
      601 tgaagatcag gaaatcttcc tcgtgggaat gtatcaccgc ctggaagtct ggggtccgtc
      661 ggagtggcga cggtatctca ccaggacgga agaccggtac gagcaggata tggcgaagat
      721 ccaaaatctt ctctagttga taggcaggcc cttgcgccgc gtgtcccgcc atcgacagtc
      781 ctccatcccg attcggacga cctccttcag acccccggtt cagaaggcat cgttttctgc
      841 gcaggagccg gttctgccgc acccagccat ccgcacgcgt actccggcaa tggccatcac
      901 gtccgctcca ccgtccgttc gagtgacggc agattccatc gcactcacgt tacccgtccc
      961 acccagcgtc aatcaccaat atgccacggt caacgggcgc cgcctgctct ccgccaaagg
     1021 ccgtgcgttc aaggaattgg tcgggcaaca aattctggtt gcgcttgccc aatcgcctca
     1081 tcgtggtact ctaagacgaa ccttgagcca ggcgagcctc tcgttatcca tccaattttt
     1141 cttcgcctcg gccttgcgtc gggataccga cggaggactc aagattgcac aagacgcatt
     1201 atgtgagggc ctgggactga acgacaatcg ggtggtcgaa actcacctct acaagtatca
     1261 ggatcgcgac aatcctcgca tggaaatact gttgtcggtg gcgacgcctg cggcacctgt
     1321 ctccgcttgt cccgcttcgc gtgaggcccc gcttgtggtg aacccggacc atcgatccga
     1381 cagccggcga gagcgatagc acagagcaga gagaccgccg gagcgcgaca ggcctgcgac
     1441 gctcatacgt tatgccatac cgacccatca ccatcgatgc cctgcaaatc gggatgcacg
     1501 tcgccaagct ggacgtggcc tggttccgtt cgcccttcat gcggcattcg ttcctgatcc
     1561 ggaccgacga acagatcgag aaattacggc gcgcgggagt gaaacacctc tccatcgacc
     1621 ccgccaaggg gctggatctc ccgggaacgc ccgcttcgac tccaaacaaa cccaacgtcg
     1681 tggtgcagcc ctcgtccaca cctacatcga gtacgccgga ggtgcgctcc ctcgccgcga
     1741 tgacgcagga actgctgacg gcgagaacgg ctcgtgccca gctcgaagcg tcggtgcaga
     1801 ccaccttctc gcggattgcc aaaaccggca tcgtcgatcc ggaggaagcc acgcacaccg
     1861 tgcacgcgat cagcgccgtc gctcaagcgc tcaacagcca tgccctcttc atggccttca
     1921 gccaggggcg cgagggccat gcgccgctca gccagcatgc ccttgccacc tgcagctttt
     1981 caatgattct ggcgcatgcg gcagactaca acctcatggc catccaggaa ttagccaccg
     2041 gcgcgctgct ccacgacatc ggcctcatgc aggtcccgcc gaacattctc caacggattc
     2101 acgacacctc gaccaccctc tccgaacaga accggcggac ctatgaagcc cacgcgcgcg
     2161 gaggcgccat tctgctcgaa cgccgggacg gcttctcacc ggcggtggaa cagatcgtgg
     2221 cggagcatca tgcctatctg aacggaagcg gattccccgc agaaaccggc ggcgcattca
     2281 cctccgacat gacacggatc gtgatggtca cggatcgcta cgacgagttg ctgactggat
     2341 tcggcggcgc ttcgccgctc accccacacc agtcattgca gcggctctac caagaagggc
     2401 aggaaggcag atacgatacc cggctgatct cgctcttcgt caaagtgatg ggaatttatc
     2461 cggtgtatag ctatgtctca ctgaccaccg gcgagcgggc catcgtgtcc gtcatcaact
     2521 caggaaaact gcaccagccc atcgtcacca tcacccacga tccatcgggg gaaccctaca
     2581 tcgtcccgct cgtcatcgat ttggccaacc aggacacgga agctcctccc cgggggatac
     2641 gttccgtgct gggtaccatt ccagcggaat tcgaacgagc ctttcactaa ccccctccat
     2701 gctacgctca tagcccacgg gagccgcctc acgtccaagg cccgacggtt cagtctgggg
     2761 cgttattcgt agcgcagggc ctcgatagga ttgagtcgcg cggctttgtt ggcaggatag
     2821 aggccgaaaa acagtccgac ggccagcgag aacacgaagg cggtcacgat cgcatcgaac
     2881 gagatgatgg tcggccatcc cgcgatcaca gtggtcagtc gagcgccgac gatgccgacg
     2941 accacgccga tcacaccgcc gaacagactc agcgtgaccg cctcgatcag aaactgcatc
     3001 agaatgtgca ggcgcttggc ccccaccgcc atgcggactc cgatttccct cgtccgctcc
     3061 gtcaccgaca ccagcagaat attcatgatg ccgatcccgc cgaccaacag cgataccgac
     3121 gcgatggcaa agagcatgac cgtcagcgtc tgactcgtac tctcctgcac ctttccgata
     3181 tcgacttgcg tccggatcgt gaaatcgtcc gcctgctcgc cctggagtcg atgtcgcgcc
     3241 cgcaagacct cccgaatctg atcgaccgcc tcgggcaaat ccgcgctctg ttccgtagat
     3301 gcaaacaagg ccccgaccga tcccaggaac tgactgccga acaccttccg ttccgcagtc
     3361 gtgaagggga taaaaatcac atcgtcctga tccgagccct gagccgactg gcccttggga
     3421 gccagcacac cgacaacctg aaacggcaca ttcttgatgc gaatcgtcgc accgatcgcc
     3481 tcctcgccgg gatcaaagag attttccagc gtcgtctgtc cgatcagcgc cacgcgagcc
     3541 gcgccctcca agtcggtgtg cgtaaacggt cctccactgg tgaacgacca gtcccgaatc
     3601 gtgagataac tcggagagat cccgttgacg ggcccgttcc agttgcggtt gccgttgacg
     3661 atctgcatca catcccgctt ggcccagccg gtgtccgaca acaaaggact ccgctttttc
     3721 aaatccgctg cgtctccgac ggtcaatgtc acggccccgc cctgcccgcc tcggaccccg
     3781 ctgacggtgg tggagcccgg catgatgatg atcacattcg tccccatgct ggccacctgc
     3841 gcctgcaccg ccgcgcgggc gccctgcccg atactcacca tcgcaatcac cgcgcccaca
     3901 ccgatgacga tgccgagcat ggtcaggccg gcgcgaagcc ggttccgact gagaatacgc
     3961 agcgcggtca tcacggtgag aagaataaaa gccggcatgg gcgtcttact gcacgacggt
     4021 cagatgaggg gctcgccgta cgtcctgcac gatctggccg tccttcatga ccagttcgcg
     4081 ggaggcgtag gcggcgatat ccgattcgtg ggtcaccagg atgatggtga tgccatcttc
     4141 acggttgagt tcctcgagaa tattcatgat ttcccggctc gatgcggtat cgaggttgcc
     4201 tgtcggttcg tcggcaaaaa gaatcgaggg ggccccgacc agcgcccgcg caatagccac
     4261 acgctgttgc tggcctccgg agagctgggt tggatagtgc tgctcccgac cggccaatcc
     4321 gactcgctgc agggctgcgg cagcctgttt tcgctgttcc ttgatcgaga ggccccgata
     4381 aaacagcggc aattgagcgt tctccaacgc gctggtccgg ggaatcagat tgaagctttg
     4441 gaatacgaag ccgatctgcc gattgcgaag ctcggccagg agatccggtt ttgccgtcgc
     4501 cacatccaaa ccatccagct gatatcgacc atgcgtgggt tggtcgagac agcccagaat
     4561 attcatcagc gtcgacttgc ccgatccgga cgtacccatg accgccacaa attcaccgcg
     4621 atcgatggtg aggttgatgc cgcgcaacgc ctgcacctca acatcgccga cacgataaat
     4681 cttccagagg tcttcgcaga cgatcaacga ggccatggag ccttcccctt agagcccgcg
     4741 atcacgccgg ggaccgcgct gttgcccgga cccgaatccc ggcggcaggt caccgctctt
     4801 gcgatcccca cgcggagagt cgatcccgat gatgacctgg tccccctcat tgatcgaagc
     4861 cgaactgatt tcagcgtaag ccccgtctga aatgcccatc tcgacaggga ctcgctccaa
     4921 ggcctcgtcg gccccctgct tccaaatggc caactgacgg gtcgccgcgt cggatgtccc
     4981 gcctgcggac atacggccgc cgccctccac cttggtgggt ctgcccgaag cctgctcatc
     5041 gcgcaccgtc ttgggcgggg taaagcgcag ggccgaattc ggcactttga gaatgttctc
     5101 tttcttcgcc acgacaatgg agacgttcgc cgtcatgccc ggcttcaagc gaaacgccgg
     5161 gttctcgaat tcgaccacaa catcgtaggt caccacattc tggatattga tcggcgcatt
     5221 tctcacctgc cgcacccgac cctgaaactg ttccccagga taggcgtcca ccgtgaacga
     5281 ggccgctttt ccctcggcga tcccgccgat gtcggcttcg ctgacgttgg tatcgacctg
     5341 catcttcgtg acatcctcgg cgatcaaaaa gagcataggg atggtaaagc tggctgcgat
     5401 ccgctgcccg acctcaacga ggcgcgagat caccacgccg tcgaccggtg aacggatcac
     5461 cgtgtacttc aaatccaact cggccgcctg caacatcgct tccgcctgct tgacggcagc
     5521 ctccgtgact ttgagctgcg ccaccgcgcc ttctgaggcg gtcaacgcga cgtccacttc
     5581 gttctgcgag atgaattgct ggccgatcag cgacttggcc cggtccagtt cacggcgccg
     5641 ttgggccaga tcgatccggg ccttatcgaa cgccgccttg gcgttcgcca gactcgcgac
     5701 cgcctgatcg cgtctggcct ggtagggaaa cggatcgata cgggccacca cctgattcgc
     5761 cgtgaccttg gaattgaaat cggcatggag gctctcgatc atgcccgaca cctgactgcc
     5821 gacctgaatc gtggtgatgg gattgatcgt tcccgtcgcc gtgaccagcg agacgatggt
     5881 gccccgttcg ataggaaccg tgcgatagcg cacaacggcc tttcgctcgc cgttgaagaa
     5941 cacatagccg gcgatggcaa ggcccagcgc aaggactccg acgatgactc cgatccgaag
     6001 catggacagg ccccttctcc ctcgaacaac gcggtcattc tagcaggaaa cgattttgcg
     6061 atgcagtacc gcgacactcg gcctggcttg gcagaacagc gccggcgcag aaaacacgaa
     6121 ggccgtttgc ctgtgacggc aaacggcctt ccgaaccgat ctgtccgcta tgagcggacg
     6181 ttcatgacac gactagggac tgaccgtgat gcggtccttg atatcgtcga agatgtcctt
     6241 cggaaccaca ttcttggcca ccagctcacc cttcacgcgg tacgggcgat tactcacgat
     6301 ccggtcggcc acatcctggc tgacacccag cgtcagaacc aaatcggtcg acggcgcttt
     6361 gttgatattc agcaaggaca ccggcggagc cgcagcctcg acaggtccgc cgaaaggagt
     6421 gggagatgac gccgacttcg ctgctgacgc accgccggac aagggcgcag tcaattccgc
     6481 gggggcggac gagctcaggg atcgcgaacg ctccttcatt tctttttgat accgcgcgac
     6541 caacgacttc agttgctcat tgtgtcggcg agcttcgtca tactcctgct tgatgtttcg
     6601 attctgcgcg ctcagctgct tgaccttgtc ttccaattcc ttggtccgga cgtcaatggt
     6661 atcgcgctcc ttgtcccgac catgctggat ccgctgcagt tcatcccggg caatctggct
     6721 ttccgacccg aacttggcgt tgagatcctt cagcgtcttg acctgttgct ccagcgcatt
     6781 cttctgcgcc cgcgcccgct ccaggtctcc ccgaacggtg tcggcatcgg caagcgcctc
     6841 atgatatttc ttgtcactta cgcacccgct gacggccatc gcactcatca gcgcgagccc
     6901 agccatccac acatgcctca tgcgaccctc cctgttcgat ctggaccgat cggacctcgt
     6961 ctctcttgtt gtgtgtatgc catcatctca actttgtcaa caagtttgcc gattgcgaag
     7021 cgcctgccgg tgagtcgctc gcagtccgcg gcgactccgc aaatgtcgac gacgcttgac
     7081 tggcagggag cagtctggga atatgcaatc accgttcagc aaaaccccgt aaccaaccac
     7141 cagcacactc acagaccatc ctaatcagac tattatgatg aactggggcc cggagctcgc
     7201 gatcattctc ggcatcatcg aaggactgac ggagtttctt cccgtctcct cgacaggcca
     7261 tctcattctc gtcgggcacg cgcttggatt caccggagac atcgcctcga acgtcgagat
     7321 ctcaatccag ctgggatcca ttctcgccat catcgcctac gaacgcacca agctggcctc
     7381 gctggcctcg cacgcattcc gtgagcagcg tgacttccgg tcgctcgtgg ccgcccgagg
     7441 gaatgcctcg tgggcaactc tcattcaaca gtccattcaa gcccagccca atctctggtt
     7501 cgtcatcggc ctaggactag cctttttacc cgcagcactc gtgggattcc tcgcccataa
     7561 gtcgatcaag gcctatcttt tctcgccgac gaccgtggcg atttcgctga tcgtgggcgg
     7621 catcatcatc ctggtggtcg aacgcatgcg ggaccgcgtg cgcgtgaagg aactgctcca
     7681 agtcaccccc cgcttggcca tctgggtcgg gattgctcaa tgcgcctccc tgattcccgg
     7741 catgtcccgt tccggctcca cgattgtcgg cggattgcta gccggcctcg accgccgcgt
     7801 cgccacggaa tattccttct tcctggcact gccgacgatg atcatcgcca ccatctacca
     7861 gatgctgaaa tcccaagccg cattcagcca ggacgattac gtcgcgctcg gcatcggcct
     7921 ggtggtctcg ttcgccgtgg cctgggccgt catcgccgcc tttctcacct atgtgcaacg
     7981 ccacagcctg agcgtcttcg cctactaccg tatggtgttg ggcgtcatcg tgctgctggt
     8041 cgtccgctaa ctacactcat accaaggagc cgagtgacca tggcaaacca aatggacagc
     8101 gtccacgtct acgacacctg ggtcaaagga aagaacggca aactccactt cgacgtgatg
     8161 accacaagtc aggagaaggc cctcgtcctc gccaagcaat atctggtggg aatcggtgaa
     8221 ccgaacgcgg tcattaccct taaggaatgc cagttctgcc acagcgaacc gctcgtcatg
     8281 ttttcggaag agcagcagcg ccagttccgg gagcagggcg gcttcatcat tacgatgccg
     8341 gtctgattgg gaaaaatgaa gcgattagtt tgaagcgctg actgtgaaga gtttcagggc
     8401 gctcaaaaag gccgaccagc aaggaaagta gcgaggcgta cgcttcggta cgttgagcta
     8461 ctggacgacg cgagaacgcc gccggcgggc tttttcagcg tcctgtcagg agggaggatt
     8521 ttcgggaacc accggagtcg cctccggctc cttctccagc gcaaagtgca gaatcagaaa
     8581 cgccaccccg actgtgatcg ccgagtcagc gacgttaaac gcggggaaat ggtaggcgtt
     8641 gaaataaaag tccagaaagt cgatcacttc cccgtagcgc aagcggtcca gtaagttccc
     8701 gatcgccccg ccgagaatcg ccgccacgct cagccgcccc atccaatcat ccttcggcat
     8761 gcggaccaga atcgtcccca gcaagcccac ggcaaagatc gaggtcacac cgaaaaagat
     8821 gaaccgaaac gagctgctgc tggaggagag aaacccgaag gccgcgccgg gattccggat
     8881 gtaggtgatg ctgaagaagt tggtgatgac cggaatcgat tcatggagcc gcatggactc
     8941 catgacggag agcttcgtga cctgatccag tacgacaatc gccagactca gtaaaccgag
     9001 caggagatag cggacggagt tgctcaacgg accgcctcaa cgcaacggtc acagagggtc
     9061 ggatggtcgg aggacgttcc gaccgacccg cgataattcc agcatcgttc gcacttcacg
     9121 cccgtcgcct tctccaccgt aatcacaaag ccaggtttct cgggcaggtc cggcgcagac
     9181 tgcaggacca catcagagac gatgaaaaac gccgggagat cctggccgta agttttcaac
     9241 agatcgtaat gggcaggatg agcggcaatc agcaccttcg cctccagcgg cgctccgatg
     9301 accttctcgc gacgttgcac ttccaacgct gcctggacgg cggttctcac ttccagcaac
     9361 tgttcccatc gcccggccag tttcgcgtcc gcccagcgcg gatcgacttc cgggaacatg
     9421 gccaggtgca cgctgtcggc cttcgagtcc acccgcaccg actccggcag catccgccag
     9481 atttcctccg ccgtgaaact caacaccggc gccatcagct tcgtgagggc caccagaata
     9541 tcgaacaaga cggtctgcga gccgcgccgt agcggcgaat ccttgcggaa cgtatagagc
     9601 cgatccttca ggatatcgag atagacagcg ctcaaatcga cggagcagaa gttgttcagc
     9661 gcatgaaaga tcgtatggaa ctcgaagtcg tcgtacccct tccgtacgcg cggaatcaaa
     9721 tcgcctaacc gcatcagcgc ccagcggtct aattcaggca actgctcgaa cggcacgcgg
     9781 tgttgcgccg gatcgaagtc gtagagattg ctcagcagga accggcaggt gttgcggatc
     9841 ttccggtagg cctcgatgag atggttcagg atctcttggg aaatccgcag gtcctcgcga
     9901 tagtcctgag ccgataccca aagacgaagg atttccgcac ccgattgctt gatcacatcc
     9961 tgcggcgcca cgacgttgcc cgccgatttc gacatcttcc gcccggcccc gtccaacacg
    10021 aatccgtgcg tgagcacggc cttgtacggc gcttgatgat cggtaatgac tcccgccagc
    10081 aaggcgctgt ggaaccagcc acgatgctgg tcggagcctt cgagatacaa atccgccgga
    10141 taccactgcc gggacttgag cacagcggca taactcacac cggactcgaa ccacacgtcc
    10201 agaatgtcgc gttccttttc aaattccgtt ccgccgcagc cttcgcattg agtcccggca
    10261 ggcagcaacg cggcagcctg gttggcaaac caatagtcgg tcccatgttg gccgatcaaa
    10321 tcggcgacat gatcgatcac ccgcggatgc gctaacacct tgccgcattc tacgcaggta
    10381 aaacccggga tcggcgtacc ccagacgcgt tgacgcgaga ggcaccaatc aggccggttt
    10441 tcgatcattc cgttgatccg gtcgcgccca taggccggaa tccagcgcac ccgttcaatc
    10501 tccgccaacg cttccttccg cagttcattc gtctccatcg agacgaacca ctgttcggtc
    10561 gcgcgaaaga tgaccggctg cttgcagcgc cagcaatgcg gataggagtg gttgagcgta
    10621 ccatggccga gtagacgtcc gttctccttg agacgctcga cgattttagg attcgctttg
    10681 aagacatgct gcccgacaaa ctcaggaacc gcggccgtga acttcccacc gttatccacc
    10741 ggcgccagga tttcgagccg ctcaccgacg ctggccttgg cattgtgatc cagcaccagc
    10801 aggtagtctt ccataccgtg gcccggcgcg atgtgcacgc agcccgttcc ctgttccaac
    10861 gtgacgaagt cgcccaggag cagcggcgaa aggccggtgg tcaagggacg ttgcgtttcc
    10921 aacccttcaa aaccctccgc gcctttttta actcccacga ccttgtaagc cgggagacta
    10981 caggccttcg ccaccgcttc aaccaacttc tcagcgataa tgagcagctc gtcgccgacc
    11041 tgcacaaagg catagtcgat gtcggcatgc aggcagacgg cctggttcgc cggcagggtc
    11101 caaggggtag tcgtccagat cagcaccgag gcgctcttca cctcggcagg aaatgcaagc
    11161 ccgccgaacc gcttgctcaa aacagccgga ggactcacca gcggaaacct cacatagacc
    11221 gagggagacg tgtgatcgtc gtattcgact tccgcttcgg ccaaggccgt ctggtcctgt
    11281 gtacaccaca agaccggctt tagtcccttg tagaccccgc cccgctccac gaaacgcccg
    11341 aactcgcgga tgatcgtgcc ttcataggca gggttcaacg tgagataggg ttgctgccag
    11401 tctcccagca cgcctagccg ctgaaactcc tcccgctgaa tcgagacata tttctcggca
    11461 tattctttac acagcttgcg gatcgcgagg gcatctaagt ccctcttctt gtcgcccagt
    11521 tccttcaaca cctgatgttc gatcggcaga ccgtggcagt cccagccggg cacgtaggga
    11581 acttgatacc ccgacatcgt cttcgacttg atgatgatgt ctttcagaat cttgttcagc
    11641 gcgtggccga tgtggatgcg gccgttcgca tagggtggcc cgtcgtgcaa cacatagcgc
    11701 gggaacccct ggcgggattc ctggatacgc tcgtacagct tttcctgcgt ccaccgggcc
    11761 agcatctccg gctcccgctg cggcagattc gccttcatcg ggaaatcggt ctttggcaag
    11821 ttgagggtcg ctttatagtc cataggatga tctatctgag gaacaaaaga gccatgaagt
    11881 gcgcgtaaag agaggactat accgaaacga ggcgagagga accagtggtt tgttagcggc
    11941 cggagccatt tagcaagatg cctggcagga tctggatgtc ctgcaagctg ttcagaaagg
    12001 ccgtccggca aggccgcagc gagcgaagat gtgaggcgta cccttgcggt acgttgaaca
    12061 tgttcgcgac gcgagaacgc cggcggcggc ctttctcaac agcttgctag ctgagggcca
    12121 tcatgcggtc caacgccacc ttcgcccact gcttttcgtc atccgggacc acaatccggt
    12181 tcacgacatg gccgtcggca aggttttcca gcgcccagca gagatgcggg gcatcgatcc
    12241 ggtacatggt cgcgcattgg cagacggtcg aggagaggaa aaacaccttc ttgtcggtct
    12301 gctcgtgctt caggcgattg accaggttca gttcggtacc aaccgcccag gtggtgcccg
    12361 ccggcgccgc cgtaatggtg cggatgatgt actccgtcga gcccaccaga tctgccttgt
    12421 tcaccacatc ctcatggcat tcgggatgga cgatgacctt cacgtccggg tactgcttgc
    12481 ggaaatagtc cacgtgcgac ggctgaaaca tctggtggac gctgcaatgg cccttccaca
    12541 gaatcagctt cgcccgctga atcgcctcca ccgaattgcc gcctcgcggc atgaagggat
    12601 cccagacaat catctgctca cgagggatgc ccatcttgtt ggctgtgttg cggcccagat
    12661 gttcgtcggg gaaaaacagg atcttctcgc gcctcgccca gctccattcg atgactttcc
    12721 gcgcattgga cgaggtgcag gtcagcccgc catgttcgcc gcagaacgct ttcaggatcg
    12781 cggcggagtt cacatagacc gccggcatga ccgtctcttc caccggcacc acccgcccga
    12841 gcgcatccca gcactgttcg acctgctcaa tcgccgccat gtcggccatg gaacaacctg
    12901 ccgccatgtc gggaagaatg accgtttgat tggagcggct caacacatcg gcggtttccg
    12961 ccatgaagtg gacgccgcaa aacacgacat agggccgatc ggaacgctgc tcggccacct
    13021 gcgcgagaat cagggaatcg ccccggaaat cggcatgctg aatgacttca tcgcgctgat
    13081 aattatggcc gagaaccatc acccggtcgc ccagcgtccg tttggccgtg cgggtccgtt
    13141 cgaagagttc ttcggcagac aaggattgat agtcggtaat gggacgggga agcgtggcga
    13201 ccgttttcac ctgcagcctt tcggttgatt gaaaaaacta taccacgagg gaacttacac
    13261 tgtctgattc tacgacacca cctcgcggct acgcaatgaa ttgagtcgga gcaaacggcc
    13321 tatgaggaac aggggttggg tcgaaggagg caggaagagc tcggtaccgg cagtaattgg
    13381 tccgccccga acgcatgagc accacgacag acttggccga ctgaaagcac atcaacttgc
    13441 gcttctggtt caaataggac aggcctcgcc ccaaaatatc ggcctccgaa ctgacaactt
    13501 gtgtgccggt ttttggagac aagggcaaac caacttatgg ttagaatttt gtcggcaagc
    13561 gagtgagact aatgcgtatt ggctgaatat agaccaaaac cacactaaga aagtagaatg
    13621 ctgcccttcc ctcgcgcgga ggcactttga tgaatatctc ccctgatagg ccaatcgaac
    13681 ttattaagaa tgatgcactt ggacgaaagc cattcgtcca gtccgttgca gcggctatta
    13741 gcggttggcg ggggaatgaa agcctaacca tcgccatttc tggaccgtgg ggtagtggca
    13801 agtcatctgt aaaaaacatg attagggatt cgttgagtct ctctcaatca gctgttcaag
    13861 ttgcagaatt caacccatgg caatgggctg ggcaggctca gctggcggaa gcattcttcc
    13921 gggaaatcga aatagcctta ggaagaagcc cctccgcaga tagcagaaag agtgctgcaa
    13981 aatggcgcct gtattctgca aagttgcgca tccgaaggct tgttacccaa actgcctcaa
    14041 acatgataac aagggtccta tcactcatcg ttggatccgg ccttgcatgg gcagtaatta
    14101 gtaaacattt gatttggacg atagtatttt tgcttttcac ggggatcatt gtcgccggcg
    14161 gtaaggtttt tgatgcgcta ggtgagtttg cttccagctg ggcccaatta aaagaggcgc
    14221 aagcggctgt acacgaaacg tctacggagg acatcaaagg ggaactgcgg cagttattga
    14281 aaaaactttc cgctccaatc cttgtcatca ttgatgacat tgaccgtctc accgcaccac
    14341 aaattggcct tctatttcag ttgataaagg caaatgccga ctttcctaat ctgatttact
    14401 tgttactgat ggaacgtgcg gtcatagaag aagctctcaa tagccaagcc tccgggaacg
    14461 ggaagtacta cctcggaaaa atagtccaag tcgatttaga tctaccgaca attcctagag
    14521 ggaagttgat tgaaatgctc gagcgcgaaa tacttgcgat gctcgactac tctcctacga
    14581 tcgatagcga tgaagaggat catcgcctct cgtccgcact tacggccatc tcgcctcatg
    14641 tgaagactat tcgctcagtc catcgcttta cgaatacgtt ggccttcgac ttgtcatcgc
    14701 ttcgcggaga cgcatcgctg ggagtgaata agatcgacct tttgtgctta gctgcagtga
    14761 aggtttttca cccccaggta tatcgccaat taccgaaaga aaagactttg ctcacggaat
    14821 cacactttcc agactatcaa aggaagagcg tgaaccgtct gaatgctttc gtactaagtc
    14881 aaacatctcc agacgaacag agttatctgc tagctctcct ggcagaactc ttccccaatt
    14941 ttccatggaa gatggaagag gaagaagatc tattcactca tcatttgtca tctcgcactg
    15001 tcactgagaa tgaagcccta acgactctct cgatttgcca tcataaaaac tttgatcgtt
    15061 attttgagca aactatccaa gacggtgata tttcagctga agaatttgat gagctaattg
    15121 aattcctccc caatcaagcg aaaacctatt cgcggctatg cgactttcac cagcgaggaa
    15181 ttctgctaag cgttctggaa agacttttgg cgtgcgtcga taatttcgat agcggagttc
    15241 tcactcctct actgactgcg ctattcgata tttcggatga atgcttgcca gagtgggaat
    15301 ctgcacaaag accaccgacg gacagacttc tgcttttttt gagctcaagt atccttcaga
    15361 gtatccagga tcgtaccgaa aggttagaaa ctttttccaa ggcagtagag acgtcgaccg
    15421 gtttatattt accggtttcc aaagtcgcgc tggaagagcc ggggagtaaa aggcaggctg
    15481 tgggtggctt gagcgagacc gagctagcac ctttgcaaag aaattgtctg aatagaataa
    15541 aaagttgggc tgaggccggc aaactgatta accaccctgc acttggctat atcatcttgc
    15601 gctggcagga atggtcgcag gatgaaacct gccgttcttg ggttgcctct caaataaact
    15661 cgaaagaagg gttgatacgc atatgcacat ccctcgaaaa ccgtcactat gatcaaaacg
    15721 gaatgacgta cagtattcgt ctcgacttac tcgaacagat tgctgacctc ggccacattg
    15781 tccataagct caaagcgttg aactatgacg cactgacaaa taaagaaaag cagggtgtgg
    15841 atcgctttca tctcgccatg caacagaggc tgaagaatga gcatgcctct taagaacgaa
    15901 acactaagct gggtactgga gcccggcttg gggtcggatc ttgaattgtg tatggccctg
    15961 aaatttggat gcggttcttc gatccttaga gtgaggggca cgtctcgcaa tcacgcactc
    16021 cacgacattt cacgtcggct ctcaagatcg cggcgaggaa tctacaactg gtgtcaggtc
    16081 ttgcaatcac acatacgaag gtgatcttgt tgggtggcag cacccaacag cgccagcaac
    16141 aggcgatcga gacggctaga gaccggtggg cagactaccg tcgccgcaag ggcaccagga
    16201 cgtaacagtg agcgggggaa cctatggcac tcacacgaca gttcaaagac acggtaacgg
    16261 cgcgcgcgga gcgtgatccg cgttttcgtg aggcgctgtt tgcggaggcg ctgaatgctt
    16321 atttctccgg cgatacgact gtgggcaagg ccatcctccg cgatctggtt aatgccacca
    16381 tcgggtttga ggagctcgcc ctgacgctca agaagccgag caaaagcttg catcggatgt
    16441 tgggtccagg ggggaatcca agcaccgaca acttcttcag tatcctgaac gcgctccaga
    16501 agaaggctcg cgtcacattg cgcgtgacgg ccaaagccag ctaagaacgc acgggcgtag
    16561 tacactggtc cgcaccattc atttcggagt tcacatgccc cacgtgttga ttattcacga
    16621 agtcgaagcc taccccgcct ggaaagctgt gttcgatggc gcggcggatc tgcggaaacg
    16681 tgccggagaa atcagctacc agctcctgcg ctacgaccaa gatgcgaata gcattgtcca
    16741 cttctcggcc tggtctagct tggagaacgc ccggcgcttc ttcgagtccc tggaattggt
    16801 cgaattgagg cgagccgccg gcgtgaaggc gccggacttt ctgtatctgg aagagatcga
    16861 gcgaggagtc ctctagccag gactattcat aaatacccgc tccggcgccc gatcctctcg
    16921 cccggcgagg ctgttctcgt tcggcctcct cgacggactg cgagtacgcc tgcgtctgcc
    16981 tcacgtgtga gcagcctcgg cgggcttccg tctcgaacgc ctcgcgacgg cattcatgaa
    17041 taatccgggc tagcaggctg ctcacaatgc ccgccggaga tctatgacac acaccgtcag
    17101 acaagccacc gtcgatgatg tcgaacaact cgttccactc ttcgacgcct atcgtcagtt
    17161 ttacggtcgg accagcgatg tggctgccgc gcgggcgttc ctactcgccc gttttgcgaa
    17221 caaagagtcc acgctgttca ttgcgcacga gggagatacg gcgatcggat ttgcacacct
    17281 ctatccgagc ttctcgtccg tgtcgctggc ccggattttt atcctcaacg atcttttcgt
    17341 gcaggagcag gcccggagaa cccgcgtggc ttcttcgctg ttgtcggctg ccgccacctt
    17401 tgcagtttca ctcggtgcgg tgcgcctctc gctctcaact gcgctcacaa acgacgccgc
    17461 ccacgcgctc tatcgttcag cgggatggaa ccgcgaccaa caattttccg tctatcatct
    17521 ggccctgtcg gtttaggcaa cgccactgtc tctccgggcc gcgccgaacc ctcgtagacg
    17581 ctcacagtca gcgggccgat gcctcacggc attggcccgc tcctctaccg ccccttccgg
    17641 cttagcgaga caccggctct tgatctttga ggctggagag tgtctcctta atcactctca
    17701 tcgtgtgctc gatatcacca ttcttttcgc gtttggcatc tcgtttgacg tcccgcggct
    17761 ctttcttcaa caccgcccca tgctccatca atgactccct gaaccggccc agataatatt
    17821 catagtcggt ccgtcccaag gtcgcgatcg cctgatcttt cttctcaagc agagcgctcg
    17881 tatagagctg ccgtgcctcc tcaatacggg gggcggcgcg acggagacgg cctccggcct
    17941 cctttgcaga cgccatctcc cccatccgat cgcccatgtc gagcaccttg taatcctttg
    18001 ccaatggcga cgccagggcc acacgcgcat ggtaggccac ggcgcgatcg ccgaaccggt
    18061 cactcacctc cctgagcgta tcgttccctc gacggagtat ggcgctgccg tcgaaattga
    18121 ggatgcgtcc gacgtcatca gagaagaagt cctgcgcaat agattcttcg tcgtagcccc
    18181 gtggaggagc gacacggatg gtcagggcct cagagacgac atcctccgtc tccatatgga
    18241 gcgcgatctg gatcgtgtaa tatcccggct cggcgatatc ccagccgttc cgcccgacag
    18301 agataaagag cgaatcggtc acaaattccc cgggcatcac cacctgagcc tgcatgtcgt
    18361 ggcaatagcg cgcaaagggc aggtactgcc gggccggctt gtcccgcttc ttgacgatga
    18421 cggtcatgtg atccgtcatg gacagcaact ccttttccag caccagtgga cgcgaaccga
    18481 tgttggtcaa cttgagctca agcacaaccg gctcaaggaa ttgataggtg gcttgggccc
    18541 gatcgacgcg aaggttgagc ttcaacgcgg gctccggcga agcacttgcc tgctcgaatc
    18601 catggtgatc gaaccaatcg gcattcccca tctgcacaaa ccgctccggc gcatggcgca
    18661 tgaataccaa ctcgttgtcg ctgaaccgat aggcgaagtc ggaaaaaaac gccgtctgtc
    18721 cgccggacac gttgtacgga taattcatga agctgcgggc ttccggttcg ttcgccaaag
    18781 gaatccacgg ggtcccccag tccggcgggt gttgcttctg ccacgaatgc gccagattga
    18841 aggcgtgtcc catttcatgg cacgccgtcc agaaccgcat ccgcttggcc cacgcgaccg
    18901 gattagcatc accggtcggc gccgtagaga tgaacgactc gttgaaaatc gctgtcccct
    18961 ggcgatggtt cggcccgata tcgtcgaaca tgatgccgcc caggcttgtg ccctgctcgt
    19021 gcaaccgggc aaagaacacc cacagggacc actgcgcctt gccggcaaat cgcgaccaat
    19081 acacttgcat cgcatcatgc atttcgttgt cgctccaacg cgcgttcgtc cccgcgtcgc
    19141 cgatagggac gatgctgtcg ctaccggact tccgcacatc gaacccgacc cttcggtaga
    19201 cctcctcgat ggtgagcgtc tcgcccggca atgtggccgg acgattcgga tggtcatggg
    19261 tgccgatgtg cgtggtcgcg gtgacgccgg tggcgcagtc gaactcgaat tccacctgtc
    19321 tgaagtacgg agatttgaac gccagcgttc tcgtccgaac cgacgtgccc ccgcctgaga
    19381 accggacaac ggccttgcgc tggctggaga gaaaggttgc cgtcgtcgtg atctcgacca
    19441 tcgtgtaggg gaagctggcg acatcgccat ccttgaacca gattgagccg gtccaggcat
    19501 tcggcccgga tgcggtgaga cgggcaatcc agtgcacacg tttggccaac acgccataga
    19561 gcgttccgct ggccatcatc tgcggatagc gcccgtcgac atcgaggcgc agctcttcgc
    19621 gaagaaagcc ccaggggagc agttcctcaa tttgccacgg accggctgct gtcggcgcga
    19681 tgcccggcat cggctgagcg agtggaacgg gctgaatcgg cagggggccg ggcgccggga
    19741 tggggggcgt cacgatccgg gatgcggcgt acaacccgct cacgcggaca cgtggcggcc
    19801 agggcaggat cggtatgagg ggctctatgc ccggacgggc tatttcggcg gcttcgacac
    19861 gatactcctg atcgatttgg tcttgctctt tcatacgcta cctcctgttg tgatagaccg
    19921 cgaccttcag gcgtcatgct gtttcggtac gtatcgtcac aggtacccga agggttcgta
    19981 cgaggttttc caggccaggt tcccttcagt ggtgagtgac acaccgagat aggtcggacg
    20041 atccaccgaa accgatttgg tttgagccac cccccgcttg ctcagcacga cctccacgac
    20101 gaccggggta tgtgcgacgt cgacttccac agcagccgca tatccggttt gtcgacgcgt
    20161 cgtgagatcg gcactgcccc acacctcccg ggcatccacc gacacacgca cctcgtcatg
    20221 atgaaagccc tcttgtagag cgatgtgcag caacacgagc cacctcgtcc gagctatgga
    20281 gtgatcaaga ttgactgggg gagaggtccc cttacactgc tgttctccct gtcaaaagca
    20341 aacggtatac cgggtgaggc gtctggtcac gcagcggtca ggagataaaa acaagtattt
    20401 ggaacctcgc gaaatgtttg cggcgaccag caggatggcg gcgggcacgg aagcacgacg
    20461 cagacttcac gggaatcttg ggaggaacca gcgggcgttc tcgtatccga tacgatacga
    20521 gggcggtatc tgatgcgata caaacggtca tcgcaacgcg gcccctcttc gctctggctt
    20581 gcaattggta ctctccgatg atgcgcaagc gtgcagcttc tcaccctgaa tgcacccggc
    20641 gttcaaccat cccttgagca tgacccattg tgcattgaac attccgcgac gaatctcccg
    20701 ccgcattatt caggccactt gcctaggcac tccgcattac tcgcccgaac tggaattccc
    20761 tgcccgagaa gccccttgac cactaggtcc tccagaagat tgacttcgcc ccggcaggtc
    20821 ggtacgatca cattcgacag ctaggggtgc catccggctg agagtccgac ttgctcgggc
    20881 gaccctcaca acctgacctg ggtaatacca gcgtagggaa gcgaccggtt gactggcttc
    20941 gtgatcgctt caagccgcac cctcgctgga tgcggctttt tttatttcgg ctgtcagacg
    21001 ctcacctccg acgaaaggat gtccgctatg agcgaggccc atacacagaa cggttccagc
    21061 aacggtcatg gctccaagcc ctcgaccgca cgcctcacta cgacgccttt tgcggcttcg
    21121 cgcaaggtgc atgttgacgg cgcgctccca ggggtccggg tcccgatgcg cgaaatcagc
    21181 ctgaccccca cgcagtcagc caacggccag ggccctacgc ccaaccagcc gatcacgatt
    21241 tacgacacct ccggtcctta taccgatccg aacgtgacga ttgatgtgcg cgccggcctg
    21301 ccgcccttgc gccgggcctg gatcgagagt cgccgggata cggaagaact ttcgcaggtc
    21361 acatcccagt acgggcgtca gcgagccgcg gacccgaaat tggccgacct ccggtttacg
    21421 catattcgca agccgctccg cgccaaagcc ggacacaacg tcagccagat gcactatgcc
    21481 aagaaaggca tcatcacgcc ggaaatggaa ttcatcgcga tccgggagaa tcagtcccgc
    21541 gcaagggcca aagaactcct ggcctcgacc aacggccatg gcggcggcgt gttgcagcat
    21601 cccggccaag cctggggcgc gcgcattcca aacatcatca cccccgaatt cgtgcgcgac
    21661 gaagtggccc gcggacgcgc gattatcccg aacaacatca accacccgga aaccgagccg
    21721 atgatcatcg gccggaactt cctcgtgaag atcaattcca acatcggcaa ttccgcggtc
    21781 gcctcctcga tcgaggaaga agtcgaaaag atgatctggt cgatccgttg gggcgccgac
    21841 acggtgatgg atctttcgac cggcaagaac attcacgaga cacgcgagtg gatcatccgc
    21901 aactcgcccg taccgatcgg cacggtgccg atctatcagg cgctcgaaaa ggtcaacggc
    21961 aaggccgagg acctgacctg ggaaattttc cgggatacgc tgatcgagca ggccgaacag
    22021 ggtgtggact acttcacaat ccacgccggt gtcctgctgc gttacgtgcc gatgacggcg
    22081 aaacgcatga ccggcatcgt ctcgcgcggc ggctcgatcc atgccaaatg gtgcctctcg
    22141 catcaccagg aaaacttcgc ttatacgcac ttcgaagaga tctgcgaaat catgaaggcc
    22201 tacgatgtgg ccttcagcct gggcgatggc ttacggcccg gttcgattgc cgatgccaac
    22261 gacgaggcgc aatttgccga attggagacc ttgggcgaac tcaccaagat cgcgtggaac
    22321 catgatgtcc aggtgatgat cgaaggtccc ggccatgtgc cgatgcatat gattcaagag
    22381 aacatggaga agcagctgaa ggagtgccag gaggcgccgt tctacacgct ggggccgctc
    22441 acgacggaca ttgcaccggg gtatgaccac attacctccg gcatcggcgc cgcgatgatc
    22501 ggctggtatg gttgcgcaat gctctgttac gtcacgccga aagaacacct cggtctgccg
    22561 accaaggaag acgtgaaggt cggtgtcgtg acgtacaaga tcgccgccca tgcggcagac
    22621 ttggccaaag gccatccggg tgcgcaggcc cgtgacaatg ccttatcaaa agcgcgcttt
    22681 gagttccgct gggaagatca attcaacctg tcgctggacc cggacaccgc ccgctcgtat
    22741 cacgatgcga cgctgccgga caatgccgcg aaggtcgcgc atttctgcag catgtgcgga
    22801 ccgcatttct gttcgatgaa gatcacgcag gacgtccgcg actatgcggc gcagaaacag
    22861 ctggaggagc agcaggccat ccagatcggc atgaaagaaa aatcagaaga attcaaaaag
    22921 gccggttcag aaatctatct ataaggcgga tcaacatggc tatccggcaa ggccacaggc
    22981 gaactcaaac cggagacgta ccctccgggg tacgttgagg attggatgga gccgagaacg
    23041 aagccggtag tcatgttcag cagccgctga gagggaaggt agcatgggaa agccgaagcc
    23101 ggcgccatta cgcgagcgcg atatcacccg ccagattgcg cgggagtact ataaagagtt
    23161 cgatcagctt atcgagagcg acgtcatcat cgtcggcgcc gggccctccg gcctcatttg
    23221 cgcccatgat cttggccgga tgggcatcaa gaccctcatc gtagagcaga gcttggccct
    23281 cggcggcggc ttctggtccg gcgggtattt gatgaacaaa gccaccatct gcgcccccgc
    23341 gcataagatt ctcaaagaag tgggcgtgcc ttgcaagcag atcaaggaat gccccggcat
    23401 gtacatggtc gatcctccgc atgccaccgg ggcgctgatt gccgccgcct acaacgccgg
    23461 cgcgaagatc atgaacctga cgcgggtcgt cgatctgatc ctgcgccgcg aaggcgtgct
    23521 tgaaggcgtc gtcgtcaaca gcaccaccgc cgaaatggcg ggccacgata ttatccatgt
    23581 cgatccgatc gccttggaga gtaaaatcgt ggtcgatgcc accggacatg atgccgtggt
    23641 ggtcaatctc ctgcataagc gggggctcta ccagcaggtg ccgggcaacg gcgcgatgtg
    23701 ggtgtcgcgg tcggaagaag aagtgatgga gcggaccggg gaagtctctc ccaattgctt
    23761 tgtgatcggg ttggctgtcg cggccgtgtt cggcacacca cgaatgggcc cggccttcgg
    23821 atcgatgctg ctctccggcc gctatggcgc ggagttaatt caaaagaaac tgaaacaagg
    23881 caagagcata tagctgatag cgaacggctg gtagcaggga acagaccatc gaactgctat
    23941 gctccatagg ccattggctc ttgtgatcac acatggatgt tcaggacttt cttatacagg
    24001 gcgacggcag cgaagaagac aagcgcgaag cctggcagct ctttcagcaa gcctacgagc
    24061 agcagatgaa ggggcagctg gaagaagcgg tcagtctcta caaactgtcg ctcgcgacgc
    24121 atcagactgc cgaagcctat acgttcctcg gatggaccta cagcttcatg ggtaagctgg
    24181 acgaggcgat cgacgaatgc cacaaggcca tcgcctgcga tccgcatttc gggaatccct
    24241 acaacgacat cggagcctac ctgatcgaaa agggtgatct ggaggaggcg attccctggt
    24301 ttcaaaaagc catgcaggcc agacggtatg agagtccggc tttcccgcat ctgaatcttg
    24361 gccgtgtcta cgaacgccag ggtaagtgga gcgaggcgat tgactgctac aaacaggcgc
    24421 tcgccctgaa cccggactat gccttggcca agaaggctct gggtcggctc atcagctcac
    24481 tgaattaggg accataggac aggaaccgga tgaacaccac gattttagtg gccgtgactg
    24541 acattttctt ttacaccaag gtacgcgatg ccttgcggcc gcagggctac acgctggagc
    24601 ggatccgcag ccaggatgag gtggctgaaa aaaccgcctc agcttcgcct gccgccctga
    24661 ttctcaacat gaacgacctg gccatcgatg ccttcaaggc gttggaaacc ctccaggccg
    24721 accccgcgtt gcggtcgctt cccatcctgg cttttgccaa tcacgaagaa acggacacct
    24781 ggcgccgagc caaggaactg ggcgtgacga aaattgtctc cagaaatgag ttctccacac
    24841 gaaccagaga actggttgaa gacatcatag ccaacagcag tcagcaatca ccggtccgct
    24901 cagagctgaa cgctgaaggc tgagagcaga gggcaagaat ccaatgaaac aatcccgact
    24961 ccacgatcag catcaacaac taggcgccat tttcgaggac accgcaggat ggtcgatgcc
    25021 ggcccattac ggcgatcccg ccgcggaata tgccgccgtc cgcggtgccg tcgggctgtc
    25081 cgacctgtcg caccgcggaa aaatccgggt gaccggcgac gatcgcatca agtggctcca
    25141 gagcatcatc agcaacgaca tcctcccgct ccaacccggc caggggcgct actccagctt
    25201 cctgacccat aaggggaaaa tgctcggcta cttccgggta tatgtgcaag ccgatgccgt
    25261 gtgggtcgaa gatgtgggcg aggtgggcga ggccacattt caggcgttgc gaaaattcct
    25321 gctctacggc accaaggcca agatggagaa ttgcggagaa agctggggtt tgttgctggt
    25381 gagcggaccg aaatcagcag aggccgttgc tgcggcattc ggtatcgagg tccgcgccct
    25441 tcagctcttg cataccctgc ccgccaccat cggcgggcag caggcgctga tcctgcgtac
    25501 cgaagaaacc ggcgaacagg actttgaagt cttgctgccg gccgatgcgg tccccgccgc
    25561 ctggaaccag ctcatgacat caggcgcacc gttcggcatc aagcccgtcg gcgcacaggc
    25621 gcgcgaactg ttgcgcatcg aagccggtct gcccaaggcc ggtccggatc tcaacgaaga
    25681 gattgtcccg ccggaagcca acctggaagg caaggcgttc agcctctcaa agggttgtta
    25741 cccgggacag gaagtcgtgg ccaggatgga tacctatggg aacgtgcgcc ggcatctggt
    25801 cggattgatc atccaggaca aaacggttcc gcccgcgggc tcgaagttat tcagcggaga
    25861 tcgggaggtg ggatgggtca gcagcgccgt cttttcgccg caacgcaatg ccgcactggc
    25921 attcggtttt ccattgcgcg acttttcggc acctgacacc acactgacgg tcgaagtggc
    25981 agggacgcgc catcaggcta ccgtccatgc cctaccgttt cagaagcact cgtagcagga
    26041 tgctgaaaac gttcgccggc ctcgttctcg cgtcattcag cgcctcaacg taccactgag
    26101 cgcacgcatc agtgcttcac cagctgcggc cctcctgaac ggccgttttg aacaccctgc
    26161 ggggcttcaa tcacacgaac cgatgaccac cctttttcga gtggcagagg tgttctactt
    26221 ttcaggatta cgcacggccg acacggctga cgcgaatccg agcaggctct tccgctcgtc
    26281 gtcatccaat gccaacaaca gatgcgtctc ttcggcgtgc atcagccgga ggctcaagaa
    26341 gggttcttcc cggcgctttt cgcggttatc ccacatggca tcgatgacct gcaccttatc
    26401 cagctgaccg acggacacca catcgtagcg gaaataggaa tccccgatga cgatgtcgaa
    26461 aatgacgttg ccggccgtga gcagcactag attgaaccgc ccctgatctt cgtacccttc
    26521 gggcaggaac ttattgatat ggcaccgcgc cactttgcga tcgcccaagg ctgcctggaa
    26581 cttgagttgc aatgcttcgt aatcgatttg gagcgccttt tcatcggcat tggtcgcccc
    26641 gaccgccgcg tccttcgcct tttcaaagat ctcgtctgct gacaacaacc ctgccatagt
    26701 tccctactcc ttcgttacaa gactgcccga tgtttattgt gtatgggcga tttagaggct
    26761 acgcgcagcc cgcctggcgc ggatcgcccg ggcgaccccg accagcgcaa tgccggccca
    26821 cgcgaattcc agcagaacaa aacccacccg tcggtcttcg atggccacaa ttcccagcag
    26881 caacgagccc aggatgttca gcgccagata ccccgcgtcc aattcacgcc aggtgccggc
    26941 ctgaatgagg ccataggcca cgagcaccat cagagctccg atcaccgata tgacctgcat
    27001 cgccatggta tgctcgggca ctccttgccg gacgccgcgt gagaacgggg tacggtacct
    27061 ggctgctgga tgtgattgca agcggaaact ccccatgaag aagcgggcaa ggagacgctg
    27121 atgggttggt gccgatgtgg ttgagcatca ctccggcgcg gtgcgtcggc ctgtctggca
    27181 ggctgcgctc gccgggccac cacattgcat ttccaggtcg atctccggca tactttgggg
    27241 caatcagggg ggataccgat gagcggacag agctgtttaa tcgacggatg tcaggcgaga
    27301 gtgtatgcgc cggcaggcac ccatgccatc tgcaaggatc actttctcgg cttcctcacg
    27361 tggaggcggc gacggggccc acagatgttt cgtaaatatg ccggcatgac gatggacgaa
    27421 cgcgatctga tcgcgggtga gtggctccag accctcggca ccaaagacct gccccacccc
    27481 attcccggct tgtaagtctc cccgcttcac ctgcaccttc ctgtcctgca ccggacggaa
    27541 ggtcgggcaa ttcaaaccgg tctcctggct gtcggccctc agcgttggcc tccccgaccg
    27601 ggacgcctca ggcccgggtc ggtctctagc agatttccct ccagcgccgc tttaagtaat
    27661 tgggcagtat tacacgcgcg caactttttc atcatgttcg agcgatgggt atcggcggtc
    27721 ttcatgctga tgttcagctg ctctgcgaga cttcgattgg tatgaccatc ccagatcagg
    27781 cgaagaattt ccaactcacg cggcgtgaga tcctccggcc ttcgcttcac ggcggccggt
    27841 gcaagagcgt cagacgatgg acgatgtgtt gtgaagaccc cgtcctccgt cacctcgtga
    27901 ttccctaccc ggctgtcttt tttttcagcc aacatcaagt ccctccttcg tcaccctttc
    27961 cgcatgcttc acttcgcgga ggatgccgct ttctttagtt cccccgcgcc aaggcaggct
    28021 tggacgtgtc ccagagacgt tgcaggtacc ccaacacagc ggtaatggct tgtgtccgcg
    28081 gctgccagga accctgtgca ttcccattga gctcgaccca agacccatcg atcttttggc
    28141 ggacaatcaa ccgctcgaac atgccgtggg tggccggttg aatggccagc agatactcct
    28201 gctcttccct gtctttgagt aagaacccct tcaccatcat gatcactcct cccttttttt
    28261 ctgcacactc aggaccctcg aatgcccgtc tccatcaaag tccccaaggt ctttgcccta
    28321 cgagacatat ccccgctata tcaagggctg tgccaagtga cgacggatag atattgttca
    28381 aaataaaaca atatgttgag caagaattct ggatttatcc caaacgccac aagttgtgcc
    28441 acaagaaaca agagtgtgcc aaaattcaag catgagacag tatcgcgaga cactttcagg
    28501 cgacagattg gccccgtgca tctgccgtat aggaacggat actggggaat cttgaggaac
    28561 tagacggcga gagggaattg tgcgtgaccg atggggcgac ggtttacaga accaaagaga
    28621 acggcaggga aactggaagc gcaaccgtca ggcgacggtc attcagcgca agcacaaccg
    28681 tcaagggaac gcgtggaaat ataggtcagg ttattggtgt accgggtaaa ccccttctgg
    28741 cggccgaaca agcgcaggag acacccctca cctttacgct gcacgaagaa actatagtcg
    28801 gtgccggagg gcgaagagac accgaagggg cgggggccca aagaaccggt cgccaacacc
    28861 acggtggtat tcacggccag gcgattcgcg atatcccctt gttcaccctc atccgtgagg
    28921 atagattgaa gctggtcttt gaccttctgc cggcaggact catccgacca cgtcgtcacc
    28981 tgagcgacgg tcgtacagcc ggagaggaag aagagtccca gcgccaacca cccggcaccg
    29041 accgtcattc gagcttcgcc ggacatccgg agcctccgca gcgatggggc taacccatca
    29101 ccggcgagcc atggtgatga atcatcaacc actcgtcgcc gatacgctca aagagattgg
    29161 tcgcgaggac acgcgtttcg accggctggt cgtcttgacg gctggtgaga tgttcggtgc
    29221 agatgaccca ccccaggtcc cctgcgacct gcacctgcac atccgacagc tcgaatttca
    29281 tggagaaggt attgttgaag atcagcaccc aggaatcgcg caccgccggc cagtcgctgc
    29341 ggaggctcca gccgggatga atgcaggtga cgtattcgag atgcgcccac actttgtcca
    29401 tctggagcat gtccaggctt tcaaaggcgg cataaaaggc gagattggca cgggtgattt
    29461 cttcgatacg ctgttcgacc acggctcgct cctgcaacgg tgcggcattc tactgcaaac
    29521 accctcgccg gcgcgagcct catttctact gtgagggggc ggattcgcgg ggagccgctg
    29581 cgccgccggg agtgggactt cccccgagaa cgcaggccgc cgcgaacccc atcagcccgc
    29641 atgggtggcg agcagcccct cttccagcgc ggtcttcaag agttgcgcaa tgttggacac
    29701 acgcaatttt ttcatcatat tggcgcggtg agcctccgcg gtcttgatgc tgatatgcag
    29761 ccgctccgcg atcgtacgat tcgtcaggcc ggcccaaacc aactgcaaga tttcctgttc
    29821 gcgaggagtc aaatcttcag gacgccgttt ggtcgccagg ccgcctccca acgcctgtcc
    29881 aatccccggt gttgaggcct gcgcattcgc ctcacgttca atctgcacaa cattctgttg
    29941 ttcactccac atggtgctct ctccttggtt ctagttcagg cccttcattc cttcacgagt
    30001 cgcggtcgga aatcccgata agatgggcga caacccttgc aggtatgtca gcaccgcccc
    30061 gattgccctt gttcgatccg tccatctccg gtgcggcact cgcgacatat cgatccaata
    30121 tccgtcaatc tgttctttca cgtcaggctg atcgaaggga ccgagggatg agggcggaat
    30181 cgtcaccaac acgtcgtgat ccgcatcgtg tgagagtaaa aatcctgcga catccatgtg
    30241 cgtcccctct tggcctcccc ccgtcgttcg actcgttact gccggtggca actcgctctc
    30301 atgcctcttc tcatatcaag gatcgtgcca tcggcgatgg tgggcgaagc atcaagaaat
    30361 actagggact tgcgccgatc gatggagaat ccatccattt cgtgcgtcaa ggacatggcg
    30421 caagacgttg tgccacacct gtgccacatg cctcgatgca cagtctcaac acatgcaccc
    30481 gcgcgcgagg ctgaagccga atctgatgcg cacggaatgg cgcgtcaggc gaagaaagta
    30541 ggaaatggtg gattcagagg aaagatctat ctgacgcgga ggtacaggtc cccgcattta
    30601 gatcgcaatc gaggcggatt cactgaagcg gagcatcccc tccgtcaccc agtgcggaca
    30661 catcgtattc agccaggcgg cgatagaagg ttcgccggct catccctaac aaccgtgctg
    30721 cctccgagcg attgccgccc gtttgttgca gcgccccgac catgcgagtc cgttcatcct
    30781 ggcgtttaaa atgcacatag gtcgccgccg gctccggatt ccggacctcc ggcggaagat
    30841 cctccacccg taccacggtc cccttacaat gaatcagggc cgactccact gcactcttga
    30901 gttctcgcac attgccgggc cacgaatggt tgatgaggac ctgcagcgct tccggactca
    30961 cctgtaacac agccttctcc atcacggagc ggaactgtcc gaggaaggca tggaccagta
    31021 acggaatgtc ggtgggccgt tcccggagcg gggcgagttg gagtcgagcc accttgatgc
    31081 gatacaacag gtcccgtctg aacgtgcctt tttccacatc ttcggcgaga ttgtggtgtg
    31141 tcgccaccac cacccgcaca tccaccttgc gcggcttgga ttctcccaac cgggtcactt
    31201 cacgttcctg cagcacacgc aggaggctgg tctgcacggc gggagaaata tcgccgatct
    31261 catctagaaa tatggtgccc ccctgcgcag cctcaaacac gccttcctga ttatcgatcg
    31321 cccccgtgaa ggcccccttc ttatgaccga agagctggct gcccagaatc gagtcggtga
    31381 acccggcgca attgacggcc agaaaagtgt gggacgcgcg gcggctggag agatgaattg
    31441 cgcgggccac caattctttt cccgtaccgg tttcgccttc gatcaacacg gtcgcatcga
    31501 cggcggcgat cttctggatc tcttcgtaga gcaccaccat cgcgggactc cggccgatca
    31561 agtcatggaa cttgccgtga tcggccaact gcgattgcaa ggcctgcgct tgatcttccg
    31621 ccgcgaagcg ccgtcgcaca ctctccaccc actgctccgc gatcgtcagc cgcagcttca
    31681 agaactccac accgacgggc ttcaccagat agtcgttgac ccccgcctgg agcgccgtac
    31741 gcaaggattc cggggaatca tgcccggtga tcatgacaat gacacagcgg tcgccgccgg
    31801 gactcgatcg gatggtccgg cacacctgca ggccgtccat tcctcccccc cgcaactccc
    31861 aatcgagcag cacgagttca tagggtcgcc gactgtaggc ctcccaggcc gactcgccgt
    31921 ccgcgcaagc cgtgacgtca tgtccccgcg cctcaatgac ctgttcgaac aggcgccgga
    31981 tatcgatatg atcctccacc agcaagacac gcatggcttg cttactcctt acacatagac
    32041 cagttgcagc cgctccagaa aacgggtgag cctgtggagc gcctcgcgaa cgcacacgcc
    32101 gtccttctga cccgccgccg tctccaaggc tcgtccatag gcgctgattg cttcgaggcc
    32161 ataggtcccc ccggccccct taaggccatg cccaatctcg cgaatcgtct caaaatctcc
    32221 tctatccaat gcgtccgcaa tctggatgag ttcctgccgt cgatgctcta ggaaccccgg
    32281 catcagggat tcaaactctc cgctcacctg caacacgacg gtgtcggctg acgcatccga
    32341 cgacgacggc gcgaccggaa cggaagtttg gaaatacagt tgtatggctt ccagcaggcg
    32401 ggcctttcgg ataggtttcg tcaaatgcgt cgtacacccc gcctccagac tccgctggac
    32461 ttcactctgc aacgcattag ccgtcagcgc cagaatcggt accggactcc ggccctgcgc
    32521 ctgctcccac gaccgaatcg ccctcgtcgc ggcataccca tccagcacgg gcatctgaat
    32581 atccatcaga accagatcgt acgagccccg ctggaacatc tcgacggcaa gttggccgtt
    32641 ggccgcggtc tcaacccgat gaggcgtcga tttaaaatag aactccatca tgcggcggtt
    32701 gtcgacaaag tcttcggcca acaagacctt cagccccggc atgtccccgg tcgaatccgg
    32761 cggctgagac agcagcgcat tgccctcagc caacgccacc ttgccgatca ccgccatcat
    32821 accgttgaac aaatccgatc gtcgaaacgg tttcgtgaga tagcgcacca ccccgcactc
    32881 acgggctcgc gcctggtcac cggcgcgccg ctccgaggtc aacatgataa tggatactcc
    32941 tgccaggccc ggagtacgtg caaccgtctc cgcgacctgc cacccgctga gtttcggcat
    33001 gcgcacatcc aagatcacca accgatacgg cactccggcc ttcgacgcgc gacgcagctc
    33061 aaacagggcc tcctcgccac cagggacctc ggccgcggga atgccccatc ccgccagcgt
    33121 ttcccgcaca atcaggcggt tggtcgcatt gtcgtccacg atcaaggtcc gcaagctcgc
    33181 caattgctcc cactgcgccg gcgggaccgc cggcgccggc tgaacatgca cagcaaactt
    33241 ggccgtgaag ctgaacgtac tccctctgcc cagttcgctg tccacccaca tcttgccgcc
    33301 catccgctca accagtcgtc gggaaatcgt caacccgagc ccggtgccgc tgtacggccg
    33361 ggtcgttgag gaatccacct gggtaaatcg ttcgaagatg gcagtcaact tgtctgtgga
    33421 aattccgatg ccggtatccc gcaccgtaaa caggatggtc ccggggccgt ccgcctgcgg
    33481 gtcccgttcc acacgcaaca ccacttcgcc ttcatcggtg aacttgatgg cattgcccaa
    33541 cagattgagc aacacctgcc ggagacgatt cggatcgccc accagcgacg tcggcacatc
    33601 cggctgaatc tggtaggcca actccaggtt tttctcgctg gcgcgcacgg caaccagttc
    33661 agccgcccgt tgcaccaggt cgttgagatc gaagtccacc aaatccagat ccaagtggcc
    33721 ggcttcgatt tttgacagat ccagcacatc gttgatcagg ctgagcaggt tgctcccggc
    33781 atcgcgaaaa atctgcacat actcccgctg atcctcgttc aagggtgttt ccgacaacag
    33841 atccgccatg ccgacgatcg cattcatcgg cgtcctgatc tcgtggctca tggtcgccag
    33901 gaagtcgctc ttggcccggt tcgcgaactc ggccgcctcc ttcgccgtca tgagcgcatt
    33961 ttcagcctcc aaccgttgtt tcgcttcgta ggccagggtc accagattcc cgacggacgt
    34021 ggcgaactgc acttcttctc gcgtccactc gcgcggcaca ccaacccgct catgacagac
    34081 cacgccggcc aatcgaccgc cgaagaaaat gggcacgtcc aacaacgaag cgatcccgag
    34141 agccccgaga tagggctgca ccaattcgct cgtgcgcgga tcagcggcaa catcatgtgc
    34201 atcgaccact tgctcctgga gcaattcggc gaagtagcga gggtactcga ccacattcaa
    34261 tcgttgcccc cgtgaatgcc gcgactgacc ttgctcatac agttccacac agtccagtat
    34321 ggttccggtt cgatccagca accagacact cgcccgatcc acggccagca ccagtgccga
    34381 cgtctgcatc agttcttcca gcgccgactg ccagtctccc gacccgatat gctggctttt
    34441 tgtcaaccgc atcaacgcat gttggtgggc ctgcagcact gcttgactgc gacgcaacgc
    34501 cgcctccgct tccgtgcgtc tcgtgatgtc cagcacgagg gtgagcacgc aggtctcccg
    34561 gccgatgcag accggctcga cattgaacag cccgtgccgc acctcgccgg atttcgtgcg
    34621 gaactctttt tccagatgtc gcaaaaaccc gtctcgcctc aatatctcgg ccaagtgccg
    34681 gcgatcctcc ggattcaccc agattccgat gtcgagcgag gacatgccga tcagctcttc
    34741 acgggaaaat ccggacaccc gcacgaacgc ctcattcacg tcgagcaccc ggccgtcctc
    34801 aagtcggctg atcgacatcc cggccggact gctgtgaaac gccttggaaa agcgctcctc
    34861 gctttcgcgc aacgcctgct cgacaccttt ccgctcggtg atatcccggg ccaccacttg
    34921 aaagccgctg acgacgcccc cctccagtaa cagctgcgta tgttgcccca accacacttc
    34981 tcgaccgtcc ttggtagcca ccggcacctc atagacggta cggggcgttt tacggataaa
    35041 ctggcggcca tagaaccgct cggcttgcgg acggcactgc ggcagcacga tctctaagta
    35101 atgccgaccg agcaactcct ccggaagata gcccaggaca cgaagcgagg tcgggttaca
    35161 gtaggtgaac ctccccgcgg catcggtccg atagatgata tcaccggcct gttccaccaa
    35221 cgcgcggtat cggtcttcgc tttctcgtgt cgcctcctca gcctgtgtcc tcgccgtgac
    35281 atccaggtga atgccggtca tccggatcat ccgcccctgc tcatcacaca ctcccgcgcc
    35341 caacgaagag atccagcgat aggacccgtc gtgatgccgg aggcgatggt ccagacgata
    35401 ctgtttggta tatccttcct gaaacaacgt gaccgtcgcc agcgcccgcg cgcgatcgtc
    35461 agggtgaaga cgcgcctccc agtcggcgac cgtcgccccg atttccgatt cggcgcatcc
    35521 aagctggctt ttccagcgcg gggaatagta ggcggtgtgt tcctgcagat cccactccca
    35581 caccccaaca tccgcacctt ccgcggccag ttcgaaccgc tcccgactct cgcgcagctc
    35641 ggcctctatc cgccgctgct cagccaattg agccgtcagc cggccgttgg cggaaaccag
    35701 attcttcgtc tgctccgcca ctcgcagctc caggcgacta gtggcggaac gaagtgcggc
    35761 ctcggcttca cgccgctgag acagcaggta ggctgccccc caaatcatga cggtacccag
    35821 ggcacgattg aacatcaccg tcgtcacatc gacaccggga ggcgcaagcc acaatcctcc
    35881 ggcaagcaag accgtgcata ctgaggcaaa ccgtagcgga gccgcaggca agatcaggcg
    35941 cgaactcaac cagagcggaa acaggtacag aagccagatc ccatgcccca gcggaatcgc
    36001 cacatctaag gcgaagactc cggcagtcac caggtaaatc gcagccagca tcctttgaat
    36061 cgtctcctca gaactccgca cgaataaccg attcgcgagt cggttttctc caaatctccc
    36121 gtaagatacg cccatgactc actgaggtaa accccggcaa tcggccctag tacggcccta
    36181 ctccatggag cagtgatcag aagaggctga aagagagacc gtcacgttca cgacggacag
    36241 acacgcaatc gcatcatgtg agacaaggag aagggaggag ggctatgagt cgagcgactc
    36301 ctgaatgtgg ccccgaatca cggagcgggc ttgcctgaca gaccgatgca gcgggacaga
    36361 actacgactt gaggagatag ggctcccaga aggcgcgatc ataccgcggc caggcatggc
    36421 aaccgaaagg tagtcggcga ttgttgagct caaagcaatg cctgggaaca acctcaaagg
    36481 cgaaccgaag tcccatgtcg aacgaggcga ccttaaactg atcgtcatat cgcaccgcct
    36541 cgtcggacca gaaatgatct tcatttttgg ttccatcggg acgtagatgc cattgcgcga
    36601 cttgtcgctt cacgccattg aatcgtttga tatatttgaa ccacttcctt gggagataca
    36661 cacctcgcag gtaccagggc gtcccggaga cgactcgctg ccaatacacg tcagggtgga
    36721 tccaatactg ttccgacgac agcacccgta ggaagcttga gacttttcgg agagacaacc
    36781 cgccgtttcc gacacgcggc gtccccaccc aggggctgtc ggcacattgc acccacggtg
    36841 cgccgatata gtcgacatct gtggcgcacc attccaacaa ctgatccgaa aacaccaggg
    36901 catcgagctg ataaatgagg atatagcgat acttccggaa agactcgtaa aaggcaggcg
    36961 acagcatcag tttcccattt gcaatcgcac tgccgaaata gcaatcgtcg aagcgttgga
    37021 tctcatagcc aggccgctca atctccagac tccgcggcac caccagaaat ttgtcgtacc
    37081 ggccgagata atgatccaga tgccggaagg agatttcttc gtccggggta aattgcgccc
    37141 tgttataggc gggcaccacc accgcgacgc ttcctctgtc gagcacaccg tcctgcgata
    37201 tcatcatccg ccctcacctc gcctacgtgg caatatcagc atcagccgct tcgatcatac
    37261 cgtgcagttc catcgtggtc gtggtcttgc ccctctcagg accgacgggc gactgatgca
    37321 tgcgcagtga caggccgcac cctgtccgag ccaggccgcg cgacggcccg caccacatga
    37381 ataatcggcc aggttatgcc actccgtcga aggccccagt ccggactatt catgaatgcc
    37441 gtcgcgagac gttcgagacg gaagcccgcc gaggctgctc gcacgtaagg ccgacgcagg
    37501 cgtactggca gtccgtcgag gaggccgaac gagaacagcc tcgccgagcg ggaggatcgg
    37561 acgccggagc aggtattcat gaatagtccg ggctaggatc gaaagcggcc ttcagggaga
    37621 ttgcagcggg ttcggctgag gattgtcgca ccgcatccat ggcgcgctgc acggcgagtc
    37681 cgtcatgaaa ggacgcatct atctccccat tcaaacttcc gcgaagacag gcctcgacaa
    37741 aactgtgcat catgcgggaa agacaatgag gttggccatc cgaggcctgg accggcaacg
    37801 gcacgacttc ccagccgggt tgcgacgggc gtgagattgt caagacatcc accgacccac
    37861 ggctcaatga agccttcagg gctccctgtc gcccgaccaa ctcgatgtac gctttgtcgc
    37921 cggaattcgg ggtcgcacga ctcgcaaaca actgtccctg aatcccacct cgacaggtaa
    37981 acgccgccga tgcgatgtcg tcggtttcca catgagcgag cgcgccggtt ctcgcatcga
    38041 tccgtttccg tggaaccaga atggtatttc ctgaaacggc ctcgatcggc ccgagcacaa
    38101 atccgacaag gtcaaagagg tgggacccaa cgtcgtacaa taccccggct ccggcggaat
    38161 cgagatgttc gcgaaagcca atcctcgaac caggtctcag gccgtcccag gaatcatagt
    38221 gcaccgccac ataatagggc tcgccgatct ccccgttcaa aagccggcgc ttcagttcct
    38281 gcacaccgta caagtagcga tacgtgaacg cagtctggtg aatcttgccg ctctgctccg
    38341 cggcccgaag catgtcagct gcttcctccg tgttcatccc gaggggtttt tcacagaaca
    38401 cgtgcttgcc ggcagcaaat gcggcctggg cctgacgggc gtgcactaca ttcggggttg
    38461 caatcgtgac tgcatcgatg tcaggccgtg cgcaaacctc ctcatagtcg agcgatactt
    38521 ccggcacacc caaccgctgc gccatcgaaa aggtccgtgc cgaatctcgc ccgcagagga
    38581 ccacaacctc cgcttgcgga tgcgactgca ggccgggcac atgacagctt tcggcaaacg
    38641 ctccggcccc gataacgccc actcttaatt tggtcattcc gccaccttgc aaaaaatgtc
    38701 acatctgacg cgacggaatc agatcccctc gcgacaggtc ggcgacaacc gtgtacggta
    38761 tgctcacaca ggatgagagc catggcaggc atctgggctc actggttcac tctagcggat
    38821 taccggaggc gtgtcgagac tgctgcttta tgggagtgag aactgcgtag atgaaccgat
    38881 gacaccgcaa acgcatgctg tacctgttta tgcggcggga agaagctatc cgccggcgtg
    38941 tttgagtcgg gccgattgcc aaccgaccaa ccagacaccg gccaggatga gcgcaatgcc
    39001 cacgatctcg cggacgccga tcgcctcacc caacaccagc gcagacagaa gcagagccga
    39061 taccgggatg agattcacga acacgccggc ccgcgaagga cccacccctt gcaccccgta
    39121 caaccaggcc tgctgcccca atgccgtagc gaacacgatc agatacccta aagccaccca
    39181 cccggagacc gggaccacac tcgctccggt caacagcaac ttctgatcgg tccacagcag
    39241 aggaatctgc aacaagaggg acagcagcag cgttgtccag ttgacggtga gcggagagag
    39301 ccgctccatc acctggcgac caccgatgga atacaacgcc caactcacca atcccaacaa
    39361 caccagtgtg ccgcccagcc agggattgtc cccgctcggc gcctcccctc cgacaccgga
    39421 cacgagtgcg actccggcaa aggagaccgc acaaccggcc gccaccgtgt tgaatggaat
    39481 gtctcgcacg agaagagacg acagcaacgc cgtaatggcc gggctggcgc cgataatgac
    39541 gccggcggtc gctgcgccga tataccgtag gccgaagagg gtcagaagat gattgcccaa
    39601 caccccgagc cccagcagcg acaacagcct gagatcgcga gcagtccatg tcgtacgacc
    39661 gccctcgctc caccaccaca gaggaatcag gatcaccagc gcgccgaagc cgcgcagcaa
    39721 ggacgtttct actgccgaaa acgcgccaag cgccagcttc tgtcccacaa tggacccgcc
    39781 ccaaaccagc gccgaggtgg tcaacgcggc ataggcggcg aataccgatg gtttctgcat
    39841 ggacgcaatt cctcaatcga cgacacacgt gggttgccac gggccgcgag caccggatgc
    39901 taggcgaatg gtgtcggggt gatgatccaa ataccgtccc ggatggtcgc caacacagca
    39961 gacacccgct ggtctgtcct gacgacccgg ttgagttcct gaattgcagc agtacggtcg
    40021 tcaggcggcg gttcgagcaa cacgtcgccg tcccacaaca cattgtcgat gagaatgacc
    40081 ccgcgcgggg acaacaactc catcgcgcgg cgatagtaat tcacgtagtt gagtttgtcg
    40141 gcgtcgataa aaatgagatc aaaagggccc gtgagtcccg ccatcgtctc caaagccggc
    40201 cccatacgga tctcgacctt tcgaccatgg ggggattgcg caaaaaaccg tcgagcaacc
    40261 gccgcggacg cgtcatcgac ttcgcacgtc gtgacctgcc cctgctcggg cagcgcttcc
    40321 gcgaaacaca gtgcgctata gcccgtgaac atgccgattt ccagcactcg cgtggcctgc
    40381 accaggcggg tcatcatatg caagaacgcc ccttcgaggg gccccaccag catacgcgca
    40441 aactccatcg tccgttcggt ctcctcgcga agcgcgcggc ggacgggagt ttcgggcaat
    40501 gaataggccg ccgcgtaggc atcaattttc ggcaacacga gatcggtcat tggtttcact
    40561 ccggcagaga gttgtgcgac aattcgcacc ggccgcggga tcataccatg gacgtgcccg
    40621 gagaaggagc ctgaaggtga agacactggc gacattggaa cccttgacga cacgtttgtt
    40681 ggaaatccgt cgcatccaga gcgcggccgc agttctctcg tgggaccagg agacgtatat
    40741 gcccgccggc ggcggggcgg cgcgcgcgga gcagatcgcg acgctcgaag gtcttgccca
    40801 tcaaagactc gtgtccgctg agctcgaaac gctgctcacc gaatggatcg accccgcgac
    40861 cggacaggcc gccgacagtt gggatgaacc ctcccgttcg ctgttgcggg aaacctggcg
    40921 cgacttcagc cgggccaaga aacttccatc cgactttgtg atccgcctca gccgcgagtg
    40981 ttccttggcc cagcaggcct gggtgacggc acgggaagaa agtcggtttt caaaatttct
    41041 gccctcactg aagattatcc tggacctcaa acgtgatgag gctcggtatt tagggtatcg
    41101 aaactcgccc tacgatgcgc tcttggatac gtatgagccg ggtgccacca tcgctcaact
    41161 cgcgccgctg ttcacacaat tacgggaacg ccttgtcccg ctcttacggc gcgttcaggc
    41221 cagtcacgtc accatcgacg accgttgcct gcaccagagt ttcgaccaat ccaagcaggt
    41281 ggaattcggt cggcttgtgc tgaccgccat gggctatgac ttcgagcgcg ggcgcctcga
    41341 tctctcggcc catcccttca cgacctcttt tcatccgacc gatgtccggg tcacgacacg
    41401 ggtcttcgag aaagatctgc cgtcgtgcct gtttagttgc atccatgaag ggggccatgg
    41461 cctgtacgat caaggcttgg atcctcgcta ttatggctcg cccctcggcg aatccgtgtc
    41521 actcggcttt cacgaaagcc agtcccgcct gtgggaaaat tgtgtcggcc ggtcccgtgc
    41581 gttctggcac tgcttctacc cgatgttgca gcacacgttc ccgcaacagt tggcggacgt
    41641 gcctctcgac cggttctatg cggccatcaa ccgggccacc ccctcgctga tccgcgtcga
    41701 agccgacgaa ttgacctata acttgcacat catgttacgc gtcgaaatcg agcaggccct
    41761 tattgaggga cgagcagaac cggatgatct gcccggcctt tggaacgaga aaatgcagtc
    41821 gtatttgggt attgtgccgg aacgggatgc cgagggggtc ctgcaggatg tgcattggtc
    41881 gatgggcgcc ttcggatatt tcccgaccta caccttgggc aacctgtatt ccgtgcaatt
    41941 cttcgaacag gccacactcg aacttccgca gctggaggag gagatgagag caggacacct
    42001 cttgcctctg cggcgatggc tcgagcagaa aatccaccgc tggggacgga tgttcacacc
    42061 cgaccatctg gcacgacggg tgacgggtag cggggtgaac cccgagccgt ttctccgtta
    42121 tctcgaaacg aaatacgcgg agctataccg gctctgatct gtgagccgct tttcactacc
    42181 ctcccatagc ccactaaccc ctgcgtctcc ccacgacgcc ctttctttca ctcaaggatt
    42241 acgacaggtc aggcacacct ctagggccac aacagggccc tttagctcta ttcgaccaat
    42301 cggtcttcgt ggtactactc cgccccacat gaaataggaa atttctctta gtttcttacc
    42361 ctgctccagg ggatgaatcg gctgagcacg tgggattagg aatcgaaaga caagtccttg
    42421 gactttcccg ttggcccgaa tgggttccgg cagaacaagg tcttcagcgt cgatgtccga
    42481 gtcagtggct gccacgacta gacgacatgc aggagcctat cgagtgttct ctccgcgtac
    42541 gacccagaca ttgaccgccc cccacgacga caccgacttg agggcggatc ggaccattct
    42601 ctgtgctcgg ccgggaacgg gcagagtgga attccggggt gctccatgag cggaacgctg
    42661 gccgtggatg cctgccaaga ctcgcacccg gcgaaagccg aagtccgaat tctgctcgcg
    42721 gatgaccacg ccttactccg tcgcggcctg cgagagttcc tacgggactt cctcgttgag
    42781 gacggactga cgcccgacat tgtcgaaaca gcctctgccc gccatgcaat cgatcacatt
    42841 cgcacaggca cctgggatct cgtcatcctg gacttgaatc tgccggacat gccgggactc
    42901 gaagtcctgc gcattctcaa atcgttgcgg ccggccctgc ctgtcctggt tgtcagcatt
    42961 tatgccgaag aacattactc ggctcgtgcc attcgagccg gtgcactcgg atacctgaca
    43021 aaagagcggg ggccgcagga acttcggatg gcggtctcac gcatccttca gggtggacac
    43081 tacctgccgc ctcccgaaac cgaacacaac cagaacgagg gaccgcaccg gaacctctcc
    43141 tccaccgaca ccctcccggc cacactctcg gatcgcgaaa ttgaagtgct gcagtggatc
    43201 gcacagggac gacgcctgac ggaaatcgcg gaacatctca atttgagcat caagacggtg
    43261 agcacctacc gttcgcggct cctcatcaaa ttaggaatga gaaccacggc agaattgatt
    43321 cgctacgccc tggaccagca gttggtctga cactgaaggc tgatcgcgtt gaccgaccct
    43381 gcacagcaat tctctccaag cggacgggca cctgtgatcg gtctgcacag actcccacgg
    43441 cggccggtca gtacaccctg caccttctca tcggaggact atggccaata ttctgatcat
    43501 caatgccgat gcgaatctga agcggcttat caagctggcg ttaccgggca tggaacattc
    43561 tgttcaagac agtgctacct tgcagaccgc ctgcgctcag tccacgtcgt ccaagctcga
    43621 cctggtgatc agtcgcttcg tcgcggacga cgatcaaggg ccgaaagact taatggaact
    43681 tcgcaaaacg ttcccgaccg ctcgcctgat cgtcacagcg gcgctgcacg atcccgcact
    43741 cgaaggcgag aaatttcagc gcgtcgtccg cgaactccac atcgagtatt gtttgctgga
    43801 accgattgag accggcgcca tgctccatac gatccagtcg gcgctcgccc ttccgaaacg
    43861 accctagatc gacccgtttt caccggtcgc ccgccaccca accgctacca caacacgtca
    43921 gcgccaacga cctaccggac cgggagcctc cggttcagta tcccatggcc gtgttgagct
    43981 tccccagctg gcttggaggc aggagaaact ccgccgacgc ttcgatgcgg gcaggaacca
    44041 gcagcgtggt gtggaacaca atatcgcgga tgggaatctt gaaatcgggt tgctgcgggg
    44101 tactgagttc gactgattcg acggactgcg taatggcagt gacatcctgc ggcccgtagg
    44161 tggtgatcac cgcctgcatg aggtcacgaa ttttctcatt ggtttcgttt ccgtcgatgc
    44221 ctttcatcag cagaggaagg acctcgtcgc gaacctggtc ggaggggctg aagttctcga
    44281 cccgttcctt gcgtcgaagg gacgtcaagt cattgtccag atcgcggctg aaggcgccat
    44341 aggcaaaccg gacgaactcg aaatgaaacc ccttcatccc ctttccaaac atctgcagtt
    44401 cgcacagaaa acagagctgc tgcagcttga cgtcactcaa caagccatag ggctcggcca
    44461 gcgataaggc atacagcagt agcactcgat cgatcgtcat ttcgtgagat ttgcgcacct
    44521 attgaatctc ctctgaatcg tccataaccg gcaggactat aaacaggcag gtttcggttc
    44581 gtcaattgat gatcgtaccg gccggatccc ctcttgaccc gccgaggcac tttcgtcttt
    44641 aatgggcgtc tttcatgccg gttcacgcgc catgagcaag actctcaaag aaatggacgt
    44701 gcttcgccag cacctggcca agcatcagtt gaagctgact cgtcaacgcg aactcatcct
    44761 gacggcgttc ctccgacaag aacacgtcac ggcagagacg atgtatcatc agctggcgaa
    44821 gaaagatccc cacctcggac tcgccacgat ttaccgcacg ctgaatctct tctgcgaagc
    44881 aggcatcgcc caagcccgcc acttcggcac ccagacgcaa tacgacaata tctcgcataa
    44941 gggccatcac gaccatctca tctgcaccgg ctgtgggact attgtagagt tcgagaattg
    45001 cgagattgaa cggctacagg aagaggtcgc gaccaagaac ggctttgtga tccaaaccca
    45061 ccggctggag ctgtacggcc tctgtgcgcg ctgccgccat tgacgaatcg cttccgcatc
    45121 cagtactatc ttgagacggc ttatcaattt caactaggat ggccattaca ggctgtcccc
    45181 actctggagg cacgccgcat ggcttcatcc tgtcgcacat ttttggcgct gacgctactc
    45241 ctcggagcgc tgacgctttc gcagtgggcc accggaaccg cggaggccgc cgagaaactc
    45301 gtcgtctatt cgggtcgcgc agaacggctc attaagccgg tgctggatga atttcaggcc
    45361 aagagtggca tccaaatcga attgctgtcg tcgggcacca ccgagttggt caaccggtta
    45421 caagccgaag gcgaccatac cccggccgac gtcttcctga cgaacgatgc cgggagcctc
    45481 gagcatgccc gggaactcaa actcctccgg cccatgaata tgcgcgaagt tgaacgagcc
    45541 atcccgtcgc aattccgcgc ggccgacaac agctggatcg gactctcagg ccgcttctgg
    45601 atcgtcgtct acaacaccaa tctcgtcaaa cccgatcaga tcaaatcact tttcgacctc
    45661 gcccagccgc aatggaaaga caaaatcgcc gttcccaatt ccggtagcga atatcttcag
    45721 gccggcgttt cagtaatcaa agccaccttc ggagacgaac ggacgaagca gtttctgcaa
    45781 ggactcaagg ccaatgcggg cacgcaggta taccaaaaga gttcgcagat tgtggacgcc
    45841 gtcgcgaagg ggcaggtcgc ggcaggcatt gtgaaccact actatatcta tcggcacctt
    45901 gccacgcagc ccaccgctcc gatcgccgcg gtcatgaccg atcagcagga aggcggtatg
    45961 ggcgccatca tgaacgtgac cggaatcggg gtcacccgcg cgtccaaaca cgttgaaagt
    46021 gccaaactcc tgatcgaatt cctggttgcc caggccgggc aaaaaatgtt cgcggatctg
    46081 gacaaggaat accctctcca tccggacgtg aaagccgatc cgacactcat cgaccggcgc
    46141 acgttccgtg cggcgcaagt ccccctggct cgactggccg aattacgcga ggccaccctc
    46201 acgctgatcg aacaggtcgg ccttcgctaa gcccttgtgt agccgcgcat gcttaccgcc
    46261 acaagagctc acgccccctc ttcgctgcaa tggatcagcc tgatcactgc cgcatttctg
    46321 gttctcccca cctgctacat catctatgtg gcgctcaccg ccgcaccgac cgtgtggagc
    46381 cgtctctggt cgacgcgcat tcccgaactt ctctggaaca ccctctcgct ggccaccggc
    46441 gtcgctttga ccacactgct gctgggcgtc tccctcgcct ggatcaccgt tcggtacgaa
    46501 tttcctggtc gccgggtctg ggaatgggcc ctcgccttgc ccctcgccat gccgacctat
    46561 gtgctggcct atgtgtatgc ccatcttctg ggaatgggcg gcccggtgga acaaggatgg
    46621 cagacgctca tggggtccga cgcgcgactg ctctcccctc aaagctttgt cggcgtcaca
    46681 ctgatcatgg ccctcgatac gtttccgttt gtgtacctgc tggtccgagg agcactgctg
    46741 aatctgaata tctcctttga ggaagtggca cgcgtctgcg gcgtttctcc ttggagcaca
    46801 ctctggcgcg tcacgctccc gctcatccgc cccgccattg ccgcaggact ggcgctcgtg
    46861 attttgtatg tgatctcgga cttcggggcg gtctctctgc tccgctacca gacactcacg
    46921 tatgcggtct atcaacagat gaccagccgc tacgaccata cgtccgccag cattctcagc
    46981 ctgctcctcg tcgtgatggc aatcatcttc ctggtcacgg aacgatggtt tcgccagcgg
    47041 agtcgcttct accaaacatc cggccgctat cgacgggcga cccggcacca cgccggcccc
    47101 gctggaacgg cactcatcac cgggtacgtc gtactggtgt tcggagccgc attcgcgatt
    47161 ccggctgtca tgttgatcca gtggagtatc gaagcgattg cacagggcgc cctggatgcg
    47221 cgttttttcg gcttcatctg gaacagcagc ttcctgtcgg cactggcagc ctgtggggcc
    47281 gtgatcatcg gacttccact ggcctacctc gccagccgcc gtccttcgcg actcaatctc
    47341 gcctgcctcc aagccgccta tgccggctat gccttgcccg gtcctgtcgc cgccctcgcg
    47401 gtccttgtgc tctttacgca cttcgtgccg gtcttgtacg gtaccgtcgc ggtgttgctc
    47461 gttgcctacg tcctgcattt tctcccggtc ggcctgcagt cgatggagcc ggctctccaa
    47521 caagtcaccc ccaatgtgga agaggtggca cgcaccctgg gctgcacgac tcgccggacc
    47581 ctccggcgcg tgacccttcc gctcattcgt aacggtttta ttgcggcctg ggtcctgatg
    47641 ttcctccaga ccatgaagga acttccagcc acattgctgc tgcggccggt aggatttgat
    47701 acactggcga ttcgtgtatg gctcgaagcc agcgaggagt attatcagct ggcggcgccc
    47761 gccgcattgt tgatcgtgct cctgagcctg ccggccttgc tgctactggt atccaaggat
    47821 tggcgcggac gtgaccaaga ggtcgttagt tgaatcccgt gaacaactca gacatccgcg
    47881 agcaccgggc acagggtgca tctacgatgc ctgacgccat ggatctcgcc gacgaacagt
    47941 ctcccgtcct tgaactacgc gggatctcct gcgcctacga gcccaatcgt cctgctgtcg
    48001 aacagatcac gctcacggtc caccagggcg aaattctctg tctcctggga ccatccgggt
    48061 gcggaaaaac caccattctc cgcgcgattg caggattcga gcgggtaacc gacgggagca
    48121 tttctctgtc cggccggctc gtgtcatcgc gcgatgtgat ggtgccgact gaacagcggc
    48181 acatcggcat ggtctttcaa gaatacgcgc tgtttccaca tctgcgcgtg gagaaaaata
    48241 tcgccttcgg tctcagccac ctctcccgcc cacagcagaa ggccatcgtc gatgacctcc
    48301 tcaccctcac cggcttgcgc ggactggaac agcggtatcc gcatgagctc tcagggggac
    48361 aacagcaacg cgtcgcgctc gcccgcgccc tcgccctgcg cccggtgttg ttgctgctcg
    48421 acgaaccgtt cagcaatctc gaccccgaca tggcgagccg tatgcgacaa gatctccacg
    48481 ccctgcttcg acagaccaag accacggcca tccttgtcac tcatgaccac gacgaagcgt
    48541 tttccatggc cgaccgtgtc gcagtcctga accggggtcg actggagcaa ttcgataccc
    48601 ctgaagcgat ttaccacgtc cccaccactc cctttgtagc cgacttcgtc ggacaggcag
    48661 attttatctc cggggtcgtg gccaatgatc tggtgaccac cgagatcggg gaattcccca
    48721 acacgcagca tctcgcgact ggaaccagcg tggtcgtgat gattcgcccc gacgacattc
    48781 atatcgtccc gaccaaaggc gcagatgccc atattctggc aaggcaattt aaaggctccg
    48841 aaaatgtgta cacgattcag ctcccctccg gacagatcgt ccacagcagc gaatcgtctc
    48901 tgagtattta tcaggttggc acggcgattg cgcttcgagt ggtagcaacc cataccgtcc
    48961 tcttcccaca accacccgaa tcgtcaggaa aaaatgaacc aggacacggc aacgccccac
    49021 ccccgtaaaa ccggactcaa tccaggcctc catgccgaga gcagggcggg ggtgccccct
    49081 tagacaacca acacgagagg cggaggatcg acgaatggat ctgctgaaga atgcggtaga
    49141 atatggaatt attggcatgc tgatagcgct cagcatctgg tcggtggcag tggcggtcga
    49201 gcggtggctc tactatcgac gcgtggacct gtcccaattc acggacattc aaacatttga
    49261 aatggcgctg accaaacgcc tggtcattat aggcacggtg gccgccaatg ccccttacat
    49321 cggattgctg gggaccgtcc tcggtattat gatgaccttc catacgatgg gaacctcagg
    49381 cacgatggcc gtgaatacca ttatgatcgg cttaagcctg gccctgaagg caacggctgt
    49441 cggcctgctc gtcgccatcc cctgcgtcgt gatgaacaat attctccggc ggcgggtcac
    49501 cgaactgctg acgatctata aggtgcaaca tggaacgcga ggttaatcaa atcaacgtca
    49561 ttccgctggt cgacgtgatg ctcgtgctgc tcgtcatcgt cctcaccacc gccaccttca
    49621 tcagcaccgg ccagatccct gtgaatctcg ccaaggccaa agaggccgga gatcataaag
    49681 atgtccccgt cgtggtgacc ttaaccgcga acggcgacct gttcctcaac gaccgccccg
    49741 tcccaccgga cggcctcaag actgtcatgc tcgcgcaccc acgcgaatcg ctggtggtcg
    49801 tacgagccga caaagtcaca ttactggaac ggtttgtctc agtggtggac gaaatacgag
    49861 gtcttggatt tcagtccgtc agtctggagg tggtacggct atgacgtccc gccagcccgg
    49921 catatcggac cccttcatcc gcttccatgc aagcggatgg atcatctcgc tctgcctgca
    49981 cggcagcgcc attctcctgg ccggactcct cgtcgccaaa atcggcctgg ctcctccatc
    50041 ttcatccttt caatgggatg tcacggtcgt cggctctcaa cctccggcga cgcctgccca
    50101 ggtcgcaccc ggtgcgcaac cacctgctac agcggttatc cgaccggctc aacgtaacgc
    50161 tccagcggcg gccctgcgtc ccacaccttc tcaggcagcg ctcccgacca taccggcaac
    50221 tacctcaagc tcttcggcaa cggtcgagcg caacaccatc gaagcagccc tcaccccgcc
    50281 tcaccaggct cccgcacaac tgcttgaacg cgctcctgtg gtgcctcctc ctcccgaact
    50341 tcaggaagca aaggatgaac ttcaaacttc cctacacacg acatcggcac cggtcgaggc
    50401 ccccgctgat ccccgctcac tgccccagat ggcgaatccg ccgcttgcag acgctcctct
    50461 cgccccagaa atgtcaggac cgacatcaca accgccttct gagctcgcca cctctccggc
    50521 tcaaacggca tcgctcgttc cctcgaacag tacgactcag gctttacgaa aaccggatta
    50581 tggctggctc gcagggcctt tgctccagcg gattgaagca ctcaaacaat atcccgccac
    50641 ggcgaggctg catcgcctgg aaggtcgggt gattgtacgg atcgtgattc agcaagacgg
    50701 atacattaca tcggccacca tcgcaagaag ctcagggcac gatgtgctcg atcaagccgc
    50761 actggaaacc attcggcagg cctcacctct caccttgtcc cagccgctcg aaaaatcctc
    50821 ggtcacgatg caaataccgc tcggctacta cctggatcga taagccgcag agttatccct
    50881 ggcagggttg ctgttcccag cctcgcaaga ccgcctgctt gtcgaacctc aagacatagg
    50941 cccggcagcc gcctccccgc acttgaccac cgaagcctgc cgttccgctg ccgcgttcga
    51001 aataggtcca gatttctccc ccgccggtag ccgcttgtgt cttatgcggt gatccatatc
    51061 gcttcccgac catctcttgc gtcgcctcgt tgaccgcatc gtgaaaatat ttggaactac
    51121 cgctgcatgc gctcaatacg agcaggccga ctactatccc tgcgcgcata ccttcccagc
    51181 gccgaccagg ggccaagccc catccggaac gtccgccttg gatggaatta ctcgatgcca
    51241 tcattcatcc tcaccctccc cttgagatac gaagaaaccc agactttgcc gtcgcgatct
    51301 acgatcaccg caccgacccc cggcaaccgc tcaaccaagg ccagtcctcg ctcacgtccc
    51361 atcacaaaaa tgccggtatc caggccatct gcggtcagtc cgtcgcgggc gaccacggtc
    51421 acgctttggc agtcacgagc cggttgcaaa gtgatcggat cgagaatgtg gtgatagcgc
    51481 actccatccc gctcgaagta ccgctcatag tcaccggccg tcgaaattgc ctcatcctga
    51541 agctcaataa aagccagcac agcctcttca tgcctcggat gccggacgcc gaagggaaat
    51601 ttcatgccgc ccggcagtct gccgaaggtc ctgatatccc ccgagagggc cacgacccct
    51661 gcagtggctc cggccgcctg catggctgcg acagccatat ccgcggcaaa gcctttgccg
    51721 atcccgccca catctacccg cattcccggc ttggccaaat agaccgtcct ctctgcgaca
    51781 ttcagatgta gcgaacgcaa ctccgtcagc gggcgaaccg cctgcaagac cgcatcggat
    51841 ggaatgtgct gccgatcgag aacactccag gcctcaaccg ccgggccgac gagaatatta
    51901 aaccctcctt ccgtcagccg agccatttcg agcgaggcct cgagcacctt catcgtgtcg
    51961 cggctgagca tgacggcgtc cctgccagcg gcagcattca cctgcgagag ttcgctggtc
    52021 gcaatccagg tactgagcaa ctcttccaag cgatgaatct cctggaatcc agccgaggcc
    52081 gcatcctgcg cccgccgacg atcagacgcc accgacgtaa tggtcacgag cgtacccatc
    52141 tgcatctgcg ttcgcttcac gacgacagga gcggattcag accttgtagt cgcacatccg
    52201 gacgagaaca agagcaggaa gatcccgctc atgactgcaa aacgatatcc catctccacc
    52261 tcaccacctc cgacgaatat gacaaacaga ctgcgcttaa cactcaatag atcacacaac
    52321 ttcattggcg cagcccggcg ctgatttacg cgcacacgcg tccatcttct gcgggacgcc
    52381 tccaaaatgc aaatgtcgcc cgtcgcgccg accggcaggc aaacactcct ttcccctacc
    52441 ccactcatcc gacaaaatca cctgtggcag gcgcgcagcg cacacaaaaa agcggccaac
    52501 ttttaattgg tcgccagact tgacattcct ccgctgatcg ggatattaat aatcgttctc
    52561 aatttcacca acgagaccga ccacccgcta tgatcacagg aaaaagtttg gcatccccta
    52621 cctgccaaga cagtgagacg cgctgcgagt gcggacaact cattgccaaa gtccgtgggc
    52681 aaggccttga gctcaaatgc aaacgctgta agcgcatcgt cgtcatccca ttcacctcaa
    52741 tcgaaggctg gggcaccgtc acagcatgat tcgatatatg aaaggaggcc tcgtcaccgg
    52801 cagaactcca ggggctcaac cacacacaca tgctttcttc gcagaccaga gacctttgag
    52861 tcccgagtcc aaccaaaacc ttactcgcgg atggaggata cccatgaaga tccgggcaat
    52921 cctcggggct ctcgtcttgg ccagcttacc ggcgatgccg gccttggccg aaaacatgac
    52981 cccggaggat atcaagaagc tagttgacga agcagttgag aagcggctgc aggaacacga
    53041 acgacgtgaa ggcggcatgc aacaagccca gggtgaagcg gcctcgactc cgcaatatcc
    53101 tgtggcatcc ggtcccataa ccgaaattcg agtcgagcga aagggcgagg aaaagattcc
    53161 gttgggattc ggatcaaccg gatccggcaa actcatttac gccaagccct tcttgagtgc
    53221 tccgaaagca acggtcggtg gatatgccga tgtcatgtac aactcactgt cccgtcagaa
    53281 cctggacaac cctagtcgta attcattcgg ccagcaacgg ctggtgccat ttatttatgc
    53341 ggacattacc gaccatatca agtttgcaac ggaaatcgag atcgagcgcg gcggctccaa
    53401 cgcgccacag ggcactgacg gttccatgca gatcgagttt gcccaattgg attacctgat
    53461 taatgaagcg atcaatctcc gagctggtat cttgttgatg ccggtcggca agtttaactt
    53521 actgcacgac tcacccttga acgatctcgt cgatcggccg atggtctctc gcatcgtcat
    53581 ccccagcacc tggttcgaag ccggtgccgg tatttacgga accctgtatc cctcgtcacg
    53641 ctcgaagctg gactacgaag tctatgccgt caatggaatg agccagacgg ccggcggcat
    53701 taccgacctt ggggtgcgaa gcgcgcgagg cagcgtctct cgggatcgcg acgacaacaa
    53761 agccgtcgtc ggccgcatcg ccttcagccc gatgctgggc attgaaatcg ccggatccgg
    53821 ctaccatgga gcgtacaagc cggccgccgg agccgtgggg gcgggctata tcgatatgtt
    53881 cgcattggac tggactctcc agaaaggccc gttcgaaatc atcggagagt cagcctggac
    53941 gagaatcagc aacaacaatg cgactggtgt cgccaaccgg gtcatcggac cggctggcat
    54001 gcaaggctac tacgttcagg ggaattatca cttcatgccg gaattcctca agaaatgggc
    54061 gcccagccat ttcaccgacg cctctacgtt tacggcagtc gtgcggtggg aacaggtcaa
    54121 cacggatacc gacgatcgca ccaagcagaa cgcgctggga ggccaacgga agcttgagcg
    54181 cttgaccctc ggtctcaatt tccggccgat tgaagacacg gtgatcaaat tcgactggca
    54241 attcaatacc cagagggacg caaagggcct tgtagccgcc ggggatctcg gcactgcagg
    54301 cagcccgatg aatggaaacg gattcttaat ccaagcagcc acgtacttct aaccggttcc
    54361 tcgtccaggc cgtcccgatc gggacggcct ggacgatacc gacagaattg atatgacaca
    54421 gcactaccgc cgactcagcg tcctcctgat tcccgctctc ctgaccgcct gcgcatcgct
    54481 gggcggtatg caggaacgat tcgccgtctg cagctacgac catgcctggg aagctgcact
    54541 tgacgcagtc aaagatcgtg cgatagcgcg gcaggataag gccgcaggcc tgatcgacac
    54601 cgcgtggctt gaaataccca tgccgggacg gacctttggt gcccttcagc gagacttggg
    54661 ggatagcaaa gatcggtccc ggatttccct ggcagtcaaa cgactcgacg atgtcacaaa
    54721 aatcggcttc atcgaagagc gacaacggtg ggcattccgt ggcggctctc gcctgttcgg
    54781 ctggaccgac accgagtcct cggcccaagt catgaccgac gtgcagaacc gcttagacac
    54841 caaattgaag gagcatggat gttcagtaca ttgagtgcgt accgcacctt gaccgcagtc
    54901 gtattgcttc tgacctcgtc gctcccggcg ctggcgacgg agaaagtctg ggacaacgaa
    54961 ttgcgccgct tcctgaaaga cgacgacttc aagtacgaag agttcatgac cgaagaggag
    55021 gccgtgaaga ccatcctccc aaaatctcaa cgtgtgcgca aggaactgat ccgactcacg
    55081 caggacagga aggaactcat cgaacagcgc atcggttgga agtttccgga agactcgttc
    55141 gatctgtata tcggggagac tggagacaaa gtcgacgggt atgcgatgat ccacaacacc
    55201 atcggcaaat ataaacccat gacctatatg gtaggagtgg atcccaaagg gaattgcacc
    55261 gacgtcgaat tgctggtgtt tcgggatgca aagggcagcg aggtcgggaa gaagcgattc
    55321 aactctcagt atgatggcaa gaccgtcacg gaccccattc gaatcaacaa agacattatc
    55381 aacatcagtg gagccacgat gtccgtccgg tcgatgaacg ccggcgtcaa acgagtgtta
    55441 gtactggtag acgaatttta cttgaagccg gccggcttag gcagcgacac ggtcgcagtg
    55501 cagaaggccg agagaggact gttggggacc ctattcggca actaaggccg tcgggacact
    55561 gcactggcat gcgaaatttt aatgctcgat tccgcctgcg tgattcggat cgtcatatca
    55621 ggttgttgta tacgcaattc ctctttttga tgctggtggg gtttcttttt tctttcttct
    55681 gggcgcacag catgaccagc ttgagcccac aaggtatcgc cgaccactac cgtggctctg
    55741 atgcgacctt cggcgaaccg atgtcgtttc gggagctggc cgaggtcacg cactttcacc
    55801 tgttcaccat gccggtcgtg tttatgattc tcgtccatgt cctctatctc acgcaggcca
    55861 gtcatgccac gcaagtctgg ttgacatggc tttcgttcgg aggcgtggcg ctggacctgc
    55921 tctccccctg gctgatcagc tacatctcac ctatttttgt gctcacaatg ctggccgggg
    55981 acactctgat gatgggcagc tttctcgcga tgatggctat ccctctgtac gaaatgtgga
    56041 ttttgaagca accgctgatg gccgggaagc gaggggaaga gtcatgaacg cctagaagaa
    56101 gtccgttgac tccgttgcgg ccagaggtat tcagcccaga gcccaagatc gtgtgcaacg
    56161 atcttgggct cttttttttc ggaggattcc catgcatcca tcgatatcgc cgatcaatgc
    56221 cacaacacag ctcatccagg aaataacccg acagcttgat gccttggatt cacttcagct
    56281 ttccaaagaa tcattacacg ctgttcgcct gttacaactt cgacgcctga cgcggaggat
    56341 cgaaaccctc attcctggac acttcggaca tggcgagcgc accgcacact atgcccgctt
    56401 gctgggcaag gccatcagcc ttacggatga ccaacacatc gagctgcact atgcagccct
    56461 gctgcacgat atcggactcc tcacgttacc cggcagactg ctcgacgaga cagcagcgca
    56521 cagcttggat gactatgccc tgatccaaag ccatccccgg gaaggcgctg cgctcctgag
    56581 cccctatcga ttcctctctg aggccgcacg cctgatcgct catcatcacg aacgatggga
    56641 cggcgccgga tatccttatg gacttcgcgg aggctatatc cccttggccg ccagaatttt
    56701 ggccattgcg gacgtcttcg acagcattgc caatcgatcg acttcgttac acagggccct
    56761 acgaacccta caagcgtcgg caggctcaca atttgatccg atactgaccg ccacattttg
    56821 tgcacgtgtt cgcgatcaag accagctccc gccgctacca ttcggtgacg cgagtgtcac
    56881 cgctctcact gttgaaccga ccctcgacca accgagtcac cagcatatgc ccttttcaat
    56941 gttctgagga gtcgaacgaa aacagtccat acccggcgta cgggctattg gcctcactga
    57001 tggagctgaa tgatgatgaa caacgcgccc gacatcgaaa ttccaactga tcgcctccgc
    57061 accctctcgt ccgagcttcg cgcacagtca ccacactggg ctgatcgtct tgagcaggcc
    57121 acgacggaac aggagctacg caatctcgtg tgcgatctct ttaacctgtc ccatgccctg
    57181 ccgtcccaca gagagtccgc taatgagaca accacgctgg ccggacgcat cacccgcttc
    57241 atcaacgaaa atctccatcg tggactgaca ctcaaagtcc tggcaaattt cctcggctat
    57301 tcggagaaat attgctctga tctgttttac cgcatcatgg gtgaatcgtt ttccgggtac
    57361 atgagacgac atcgggtcga acgggctcaa tcactcctca ccacaaccgc gcagacgttg
    57421 gcagaagttg ctacagcggt ggggttcagc gatcagtttg ccttcagcca tttcttcaaa
    57481 cgcgcgaccg gacattctcc cagggagatt cgatcccggc aggcacactc acggcatcgg
    57541 ctgacggtcc acgccatgca gaaagcactg tagccagcgg ccagaccaac cacacggctt
    57601 cactgcttgg agaacgggaa tgtcccctgc cctgaatggc catggaatac agaacgatcg
    57661 agtcgcttcg cagcagtgag gggagaatga atgcgcgggt gattcaaatt accggacggc
    57721 ggggttcagc gaagcaggtg cgtggggcga gaagacaaca gaggagagag ccggccagcg
    57781 gggccttcgg ggatcccacc ggccgaccac ctgcgcacgc aagggctaag acttctcaga
    57841 atgaatctgc tcggccaagt atcgatcgag acctacttgc ctgatggtgc agagctgagt
    57901 ctcaaaccaa tcgatatgct cttccgtatc gacgatcatc tcttcgagcc gacgacgggt
    57961 cgtgaagtca gcgacctttg tgcaatgggc aatcccgtcg cggaggacgt ccgcgtcgtc
    58021 acgctcgaag gccagatcgg cctccagcag atccgacact ttcttgccgg ccgcaaccgc
    58081 atccaaatga tccatctccg gcgcgccacc caggtacaga atatgatcga tcaaacccga
    58141 ggaatgactc acttcctcct gagccaggtg attgaaatgt tcgtgcaacc ggccgtagcc
    58201 ccaatgtttg cacaaggccg catgcagaag atattggtgc accgccgtga gctcactcac
    58261 cagcactttg ttcaaaatat cgagaacgcc ctctttcgct ttcatgcata ctcccattcc
    58321 cacgccgggc cggcgctcgc ttaactctct ttgtgaatct gctcagccag gtaattctca
    58381 agccccactt gcttgatcgt ctcaatttgg gtctcgatcc agtcgatgtg ctcatccaca
    58441 tccttcgcca tgtcttcaaa catgtggcgc gtggtgaaat ctcccacctt cgtacaatgc
    58501 accaccccgt cgctcagaag ggtgagcatt tcctgttcgg ccttgagatc aagcttcaac
    58561 tgctcgggaa cagtttcccc gacatgcacc gtgttcatac gctgaacatt cggcacccct
    58621 tccaagtaca gaatgtgact gatcagttcg tccgcatctt tcatttcatc gatgctacgt
    58681 tctcgaacct tgtgatgcag ccgctcataa ccccagttcg agcacatttt cgcatggacg
    58741 aaatattgat tgatcgcggt gagatccgcg gtgaggactt tatttagaat ctcaatgacc
    58801 ccttgtttgg ctttcatagg tccctccctc cagccttgga gtgcaatgct ttccgccggc
    58861 atgttggtcc acgccgacgc tgacgtgcga tcgaccgact ctttactcta caccttaatt
    58921 agagattctc gaagcgcttg tcaactagag aagtacgtag aaatgaggcg agttatcaac
    58981 atcgccgaga acgcttctca ttcttctcat caggacaaga tcgggaacgg cgtgtcccgc
    59041 aagagctcgg cggggacacg aactggagtc gaggggggag atgcggaaca actgatcgaa
    59101 acgagcgaac cgcgacagag gaacggatgc gccttgacta tcctgcttcc cgatagccgc
    59161 actcttcttc attgcggcat tgcactgaac ggccggcctg cttgctgacc ttctcaataa
    59221 gaaacggcgc ctgacatttg ggacagggct tattgatggg gcggtcccag agcgcatact
    59281 cacattgcgg gtaacgactg cacgaataaa aatttcgccc ctttttcgtg cgcttctgaa
    59341 ccagatcccc cccgcacccg ggctgaggac atttcacacc cagagcaatg gctttggtcg
    59401 tcttacattc cggatacgcg gaacaggcca ggaacttacc gaagcggccc gatttcacca
    59461 ccatcgggct ggagcacttt tcgcacttct cgtccgtcgt ctcttccagc ttgggaacaa
    59521 tcttaatcgt cccatccggc aacttttcaa aattttgcgt attcttgcag ggaggatcgt
    59581 ccttataggc ggggcacgcg aggaacttcc cattccgtcc ccatttgatc tccaacatgc
    59641 ggccgcattt ttcacaagga aggttcgtgg gcggctcgac tatatccttc ggtccaggga
    59701 taccttgagc gagttccatt tccctcacaa agggtgtgta aaaatcgcgc accgctgaaa
    59761 cccagggctt tgtgccctct tcgacttcat cgagctgctc ttccatatgg gatgtaaatt
    59821 ccgtatccag cagatccgga aatcccttca acagaaagtc attcaccgtt ctcccggttt
    59881 cggtcgggag aaaccgccct tcgaccttct cgacatactt ccggtcttgg atcgtcgaaa
    59941 tgatcgaagc gtaggtggag ggccggccga tccccttttc ttccagctct cgaatcagca
    60001 atgcttcatt gtaccgggga ggcggctgag tgaaatgctg tttagaaacg agcccgggca
    60061 aggtctgggc ttcctgagcg accagatgca aggattcacc ttcgcgcaat gcgggcagct
    60121 ggcgatctga ttcgtcctcc gcctcttgct cattcttggg cttgtgcgac ggcaattcct
    60181 tgtcggttcc ctctaagtac accgccgtat ggccggcgaa cttgaccacg gtgccggtcg
    60241 tgcgaaacac atatcgatcc ggagttccca ccggagacga atccacacgc gtcacatcca
    60301 tgatcgcagg gaccatctgt gaggcaatga agcgattcca aatcagctta tacaggttgt
    60361 actggtcctg ctctaggtat tgcctgatgg attcgggatc gcgcgccgcg gacgtcggcc
    60421 gaatggcttc atgcgcctct tgcgcggctt tctgggtttt atacacgttg ggcgtagcag
    60481 gcagatactc cactccgaac cgctcccgaa tcacaactct ggcgtcttcg gtcgcttctt
    60541 gcgaaatcct cggcgagtcc gttctcatgt aggtaatgag accggtgggc ccttctgccc
    60601 cgatttcaat gccttcatac aactgctgcg ccagcgtcat cgtcttcttc gggctgaaat
    60661 gcagctttct ggcggcttcc tgctgaagcc ggctcgtgat gaagggtgcg accggattgc
    60721 gtttcttttc cttgcgctcg atcgattgca cgacgaacgg cttcccctgc acggcatcca
    60781 ggatctgctg ggcccgctcg cccgtgccga tatcggcatc ctgaccgttc acggaatgca
    60841 atttggcttc gaacgctggt ggattgtccc cagccaacaa cgcaacgata gaccagtatt
    60901 cttccgcccg gaaggcttcg cgctccttct cccggtcgca aatcaaccgc acggccacgg
    60961 attgcacccg gcccatgctg agaccacggc gcaccttttt ccacagcaac tgactgccct
    61021 ggtaccccac gatgcgatcg aggactcgtc tcgcctgctg agcctggacc agcttcatat
    61081 cgatttcacc cggcgacttc agtgcccgct tgatcgccga ttccgtgatc tcattgaaca
    61141 gcacacgata gactttgccg tctttcttct ttcccttgcc gtgcagttcc tgcgcaatat
    61201 gccaggcaat cgcctcgccc tcgcgatcag ggtccggcgc gagaaacacg gtgtcgactt
    61261 cctgggcttt ctttttaatg tcggcaagga ctttcgactt gcccttgatc gtgacgtact
    61321 ggggttcaaa atcgttttcg agatcaacgc ccagcttgct ggtggggagg tctttgacat
    61381 ggccgacaga ggccatgacg gagtagccac gaccgagata tttcgtgatg gtccgcgcct
    61441 tggtcggcga ctcaacgatg atcagcgatt tggccatgcg gctccacgtt cctcagtccc
    61501 tacgcccaga ctaccacacc cactataaca aatatcgagc ccttgtcagc tctaatctct
    61561 caatgcatgc ccgatgtgac acgcacgtac tcgttgccgg gtagctgacg aatactattg
    61621 ttcaattcga gcgataacag gatcgccgcc acttgagcgg caggcaatcc actgcgccga
    61681 atcacatcgt cgaccgattg cggcaagacg gacaagacat cgtataccaa cgtttcatcc
    61741 gggctgaatt gcaccttcgg ctgggcgacg gtccctgctc cccctgccag ccgctcacgg
    61801 aaggccggat caagctgcgg caacaattcc tccagaatat cctgcgccga ctcaaccaag
    61861 cgggcaccat ccttgatcaa actgttcggg ccacgactct gttcagcttt gacaaaaccc
    61921 ggcaccgcaa acacttcccg cccctgctca ccggccaatc tcgccgtaat aagagacccg
    61981 ctctcaatgg ccgcttccgt cactactacg cccaacgaca atccgctgat gatgcgattg
    62041 cgccgcggga agtgatagct gtgcggtgcc gcccccaacg ggagttcaga caacacggct
    62101 ccctcctgtt caatcgtctc ccgcaactgc cgatggtcag ccggataggt ccgatccagt
    62161 ccgcagccca tgacggcaag cgttcgcccc ttaccggcaa gggccccgcg atgcgcggcc
    62221 gcatcgactc cacgcgccaa accactgacg atcgtaaaac ccatcttcac caagtcgcgc
    62281 gccaactcct cagccacgat gcgcccggcg gcactgactt ttctcgtgcc gacaatcgcc
    62341 acggcatgcc gatcggtttc cagcagcgag ccctgaacgt acaacaacgg gggcgggtcc
    62401 ggaatcgtcc tgagcggcgc ggggtactgt gcatcaagat acgtcagcac cgtaaccttc
    62461 cggcgctgca gctgctctaa ttccttgtcg agttcccgga tcgcatccgg agccggcccg
    62521 cgacgaatcg cctcgactag aggcggccgg catccaatct cctccaacgc cgactgcggc
    62581 gccgagagca cagcatccgg cgccccgaat gcatgcacaa gcttgagaag gatggcgtcg
    62641 cccacaccgg gaatcgcgcg caacaccaac cagggacgga gagatcgatg actcactatc
    62701 gtctcaactc atcacacgtc gtcagtgcga tgtcataccc gttgtactgg agaaagaccg
    62761 cgttcagcgg caggatgccc acccggaaca ggtacacgcc cggtcgctcg gcggattaga
    62821 tcacgaccag atcgaattgc tccagatgca accgatcgtc gggcgtgacc agaatttttg
    62881 cgctatatcg ccgttcgagt tcctcgacgc ccggttgctc ctgttcctgc aagagcagag
    62941 caacggcagg atgcgcgccg acaaccacgc gctgctgctc gatctcatgg ccgagccgcc
    63001 gcacctcacg gaaaatctcg taggccaccg acatcggcga cttggtgtag ccgcgaccgt
    63061 cgcaatagtg gcagggctcc gacaacgaac gcagcaagtc ctctcgcaca cgctcacggg
    63121 aaatttcgat caacccgaga tcggacaccc tggaaattct ggtgcgagct ttatcagacg
    63181 acatggcatc caccagcgca tggtaaacct tgtcgcgatt tttctcccgc tccatgtcga
    63241 tgaagtccac gatgatgatg ccgccgattc cccgtaactt cacctgatag gcgatttcgc
    63301 gcgccgcttc caggttgttt ctgaggatcg tttcttcctg atcgcgcttc ccgacaaacc
    63361 ggccggtatt cacgtcgatc accgtcatag cctcggtatg gtcgatgacc aaataccctc
    63421 cggacttgag ccacaccttg cggctcatcg cacgggcaat ttcctgctcc acgcccaggt
    63481 aatcgaacag gctttcttct ttgtcgtaga aatgaatccg cgaggtttgc tccggcgaaa
    63541 accgttgcac gaaccccttg atcgcgttgt actcctcgcg ggaatcgatc aataaccggt
    63601 ccacccgacg accgaagaga tcccgcacga cgcggaagct cagactcaaa tcgctgtgca
    63661 acagactcgg agccggctgc tgatcacgct tcgtcaatac gtcctgccat aagacatgca
    63721 ggaactcgac gtccgagcgc agctcctctt ccttgacccc ttcactgact gtccgcacga
    63781 tgtagccgaa ccccgggcgg cgcgcccgct tcatgatttc tttcaatcgc gcccgctcct
    63841 catcacgagg gatacgacgg gagacgccga tatgctcgac cgtcggcata aacaccaggt
    63901 accggcccgg caaggataca taggtcgtca cgcgcgatcc cttggtgccg atcggacctt
    63961 tggagatctg caccatgact tcctgtccct cggacagcaa ctcttcaatg ggttttgaac
    64021 tttgccgttt tggcttgggg acctccgagt ccttgttatc gtcgtcgtcg ccctctacga
    64081 gggtatcccc gggttcgaca tccatcgaga ggtcggagac gtgcatgaat gccgcttttt
    64141 ccaacccgat atcgatgaac gccgcctgca tgccgggcag cactttcgcc acacgaccct
    64201 tataaatatt cccgacgaaa tcctggtctt tggcgcggtc gccatagagg tcggtgacaa
    64261 ccccgttgtc gagtaccgcc acgcgggttt cttcccgcgc tatactgatt gctatctcca
    64321 cacccataga aactccttta ccctcagatc gcgcggatcg gcagacgaac gcggacgcac
    64381 agcccgggtt cttcctcgtc tcgccgtcag tccggcacca atctggttca catggttatt
    64441 tcatacccag tcggcccctg tccaggcatg gcctaataga acaaggtcaa ccgacgtcgt
    64501 ttgacgttca acaataatcc caatgaagcc atggtcatga tcgtggcgct ccctccgtaa
    64561 ctgaccaacg gaagcggaat gccgacaatg ggaaacatcc ctgcggtcat cccgatgttc
    64621 acgaccacac aaaaacagag catcgccaca atccctgccg cgagtaacgc ccccaaggtg
    64681 tccttcgcac gcgcggcaat ttccaatgag acccatatca gcgcaataaa cagcgccaag
    64741 agcaccagca ctccgacaaa gccccattct tccgcgtaca ccgcaaagac gaagtcggta
    64801 tgcccttcgg gaaggaactt cagctggctt tgtgtgccgc cgtacagccc ttttcccgac
    64861 aactccccgg agccgatggc aattctggac tggagcgcat gatagccttt tcctcctgga
    64921 tcgtactctg gatcgacaaa ggccatgaca cgctcccgct gatagtcgtg cagggaagcc
    64981 cagaccattt cccatacaaa cgggaagagc atgacggaga gcagcaaaat cacccccaac
    65041 gtcttcgaac gcacaccgac catcaacaac atggccgcgt agacggccag aaaactcaac
    65101 ccgctcccca gatcaggctg cttgaggatc aacagcaggc cgggcaacac aatcaacccc
    65161 ggcagaacca cccggtgcag ccaccccgcg cgagacaccc gggagtaata gttcgccagc
    65221 accaacacca ggaccagttt ggcaaattca gacggttgga acgcgaaggg cccgatcgga
    65281 atccaccgtt gggcaccacg gctggactta cccatgacca acacgaccgc caacataatc
    65341 agaatcaccg cataagtcgg ataggccagg cgcgcaatct tgtgatagtc ggacaggtac
    65401 atcaccagga acgcgatcgt gcccagcaga atccacacga gctgtttgag ataaaaagga
    65461 aaggcggtgt cctgagaatg cgtgacgctg tagatcgaca gcaccccgac cgacaggatg
    65521 accgcgatca accccatgaa acggaggtcg aaactatcta accctcgact gtcgattacc
    65581 cgatcaatca tcacgtcttt ggtcgcgcgt cagcggattt cgtccgccca ccagcaggaa
    65641 ctccattgag gacctcggga taggccttca cgtaggcttc aatcacatcc ttcgccaacg
    65701 gggccgcggc ggatccgcca tgccccatgt gctcagccag gaccgccacg gcaatgcgcg
    65761 gagcctccac gggagcaaaa gcgacgaacc acgcatggtc ccgaagcctt ttgggaatat
    65821 ctttctccgg accggtacgg agggccgccg tttgagccgt tccggtcttc cctccgattg
    65881 tcacgaggga cgacttcgct cgcgtcgcgg ttcctttggt caccacatcc gccagcgccg
    65941 ccttgatcaa agcgatggtg gccggcttca ccgccacctt gccccggacc gtcggaggga
    66001 attcggcccg ttctcccgtc gcgcgattca tcaccgcctg caccaggcgc ggtttgatcg
    66061 acaggccgtc gttggccacc gtaccgatga cgctggccat ctgcaagggt gtcacagtga
    66121 catacccctg cccaatcgcg gcagaaatcg tttcaccggg ataccagggc tggttgcggg
    66181 ctttctgctt ccaggctgtg gaaggcacga ttccaacacg ttcggacggg agttcgactc
    66241 cggtctcctg cccgagcccg aactgcttcg catactcggc gatcgtatcg atccccattc
    66301 gctgcccgac ggtatagaaa tagacgtcgc acgagtggat caaagcctcg ttcatatcga
    66361 ctgacccatg accgccttgc ttccagtcgt gaaacagccg attcccgaat tgatatccgc
    66421 cgttacaccg aaccatggtc gagggagtca cggtgttgga ttcaagcgcc gccgccgcca
    66481 tgacgacctt gaacgtcgag cctggagggt attgaccttg cgtggcccga ttattcaacg
    66541 ggcgccgctc gttctggacg atttcaaccc actgtttgtt cgtgagttct ttcgacaaca
    66601 tgttcggatc gaatcccgga cggctggcca tcgccagaat atccccgttg gtcggatcca
    66661 gcgcgacgat ggcacccgac tcctccccaa gaagatcttc ggcggttttc tgaagccggg
    66721 catcgatggt gagatacaga tcgtctccgg cgaccggctt gtccgacacc acggcgcgtt
    66781 tttcgtggcc gagtgcatcg acttccacgc tcttctgccc cgcgcgcccg cgcacctgcc
    66841 ggtcgaacca cttctctact ccatactgtc cgaccacgct gccttgatgg agatcggcaa
    66901 agtccgcctt ttcaagttga tccgccgaca cttctcccac atatcccagt agatgcgccg
    66961 caaccggccc gccggggtag ttccgttgcg actccacttg aatcatcaca ccgggcaaat
    67021 ccaaccgatg agactcgatc aatgtcgcct cgcgcaacgt cagccggtct ttcaccttgc
    67081 gaggctgcag cttgctgccc ttgccggtcg acagcttctt ttgaatcacc tccggctcaa
    67141 ggttgagcaa cgaggccaat tgcgcaacca aggccggccg atccttcacg tcttccagcg
    67201 tgacatagag ggcaaagctc ggcacattgt tcgccagcaa cacaccgttc cgatcgaaga
    67261 taagaccgcg ggccggctcc aacaccaccg agcgcgtccg gttgttttca gacaggtccc
    67321 ggtaataggg gccttcccga atctggagat gccacagacg caaggcgagc aacgcgacga
    67381 ccagcaggag gcccacacgc agaatgacca gcctccgctg gagatcgagg agatccgaat
    67441 cgttaaaccc tatcgaggcc atggtcgcca gactttttcc gattgttgcc gggacacccg
    67501 cacgcgatta caacgccccc tccgtcacca tgcgatcgtc ggaccaacgc tgacgaaaca
    67561 acagatacaa gccagtcccg acaagtgcgt cgaacccggc ctgcacgagc accgtcgaac
    67621 gcacgcccat ccagacctca tcgatacccg ccggattcat cgagtagaga aacacggcac
    67681 tggacaggca ggaaatgatc gccaacccgg cggtcaacac cgccggagtg atatgggcca
    67741 tatggcggcc cgccaacccg gccagaaacc cgcctccgcc cttggtgacc acattgatcc
    67801 agagatctcc cgcagacaac atatcctgaa aacaccccag caggagcccg agaatcaatc
    67861 cgtccaactc gccgccgaga aacccgacga gacaggcggc caccagaccg aggtcaggac
    67921 gcacaccaaa gatgctgaag tagtgcaaca acgtggtttg aaagggaacg accaccagcg
    67981 ccaggagcag gtacagcaga aacttcatgg cttttttcga tccccgcgaa tctcctgcag
    68041 cagtttttgc gctgagtcgg catcctcata cggagccgtg ataatgagga cttcatccag
    68101 ctttgaaagg tccacttccg gctcgatttc ggccgactga aacagatcac cctccgactt
    68161 ctcaacctgg gtcagtcccc cgatcgccag accgcgcgga aaggtcccgg tcaatcctga
    68221 ggtgacgacc cggtcgcccg cctgaacccg cgacaacaga gggatatatt tgagtcgcgc
    68281 ccgcccatgg ctggtgccct ccacgatacc ttcatcgcga gtccgttgca ccactcccgc
    68341 aatcgcgttg ttgggatcgg tcaccaggag caccaccgag gatgtggaat tggtcttcac
    68401 gattcgtccc accacacctg cgggggtcac cacccccatc tccacacgaa ccccgtcgct
    68461 ttcgccctta ttgagaatca tcccgccata ccaattcgtg gcatctctgc cgatcacctg
    68521 agccgccaga gtctgggacg gcgattgctg cttaaagttc agcagcgtct cgtatcgtcg
    68581 cgtggccgcg acggattcgc gcagctgatt gttctgtccc ttgagcaact cgaggtcgcg
    68641 atgaagctgg cggttctctt cctggacact ctgcagggca agatacgcat tccaggtttc
    68701 tgaaatgccg tgatcgacag aagaaaacgc ggcgagagga acactgagga tctgaccgag
    68761 cggtccgccg acgtactgaa gcaacctctg gctttgacta ggaagaagaa aaagggcgac
    68821 gagcaagcag gcgaacagca ctatagcaag acgcctggtg ccgtatgttg agcgcgatat
    68881 agccatccac actcgggatg gagatcatcg agaggtactg cactgcgaca taaccgatac
    68941 cttacgaagg aggtccagct cgtcaagaat cttcccgacc cccagaacca cagaggtcaa
    69001 cgggtcatcg accgtaatga tcggaagatt tgtttcttca cggaaccgag tatccattcc
    69061 cttcagcaag gatccgccgc cagtcagtac gataccgcga tcgatgatgt ctccggccaa
    69121 ttcaggcggt gtgttttcaa gcgccacctt gatggcattg acgatggttc caatgggttc
    69181 ctgcaacgct tctctgactt cggcatcatc caccaccagc gtgcggggaa taccggaaat
    69241 cagatcgcgt cctttgatca tcatagtctt gcgctcctcg aaggggtacg ccgaaccgat
    69301 ctcgaacttg atacgctcgg ccatgtgctc cccgatgagg agattatact tcttcttgat
    69361 gtagttcatg atggcatcgt ccatccggtc tccggccacc ttgaccgact cgctatacac
    69421 gatgccgccc agggagatga ccgcgatgtc cgtcgtacca ccgccgatat ccaccaccat
    69481 gttcccggac ggttcggtaa tcggcaaccc tgcaccgatc gccgccgcga ccggctcctc
    69541 gatcaaatac acttcgcggg ctcccgccag ttcggccgaa tcacggacgg cccgttgttc
    69601 tacctgcgta atccgcgacg gtacaccgat gatgatgcgg ggccgcacaa acgccgttcg
    69661 gttatgcgcc ttctgaatga agtggctgag catcgcctcg gctttttcga aatcggcgat
    69721 gacgccttcc ttcatcggac gtaccgcaag gatattcccc ggggtgcgtc ccaacatgcg
    69781 cttcgcatcg gcgccgaccg ccatcacacg ctccgtcttt ttctccacgg cgaccacgga
    69841 aggctcgttt aagacgatgc cttttccctg gacatacacg agagttgtcg ccgtccccaa
    69901 atcgatcgcc agatcgttgg agaaccaccc gaacatgtca ctcatgaagc ccactaggac
    69961 tctcccttca aacgattcct gcgatcaacc acaccgtccc gcttaatccg acacatccag
    70021 ttgtccgctc tccagcacct gggttttggc gatctcatcg cccaatcgcc ggccctgctc
    70081 attcccgatg accagcaacc cttccagcga gagcacggcg ccccatccga tccatccgac
    70141 atacggtatt tcaaatgcca actgggcaag accgcaaggc agattacgga tgatcgattc
    70201 tcggaaccct gccgggtccc gggtacgggg cacgatggtc tggagcccga tgagccgctt
    70261 cccgacactg cgaccgccgc cgaatccgtc ggccagcaag agataggcaa gtccggccag
    70321 gacaccgatc ggaggaacca acttactcac cgccgcgacg atcaggagat cgatcatttt
    70381 ggcaatgaag cgattcagca cctgggcctt gggatagacg cccagttcga gcgggctgtg
    70441 cccgattgcc tcctccacca ccggagatct ctccaattct tcgacactat agcaaatctc
    70501 tttcacctgc acaaacaaag tcgcaatctt gccgcgcgcg ccgttcatgc gtcttcctct
    70561 tgcctttgtg atcgtcgcct gagtagaatc cgcgaaacct tgccgacaag aggcgcgctc
    70621 gatgatcaaa ctgttacgcg aagctgctca cgactatcca tggttcctca aatccatcat
    70681 gggcctgctc gccctcgcgt tcgtcattac gatgggctgg tggggcttcg gccaggaagg
    70741 cggcaacgtc gtcgcctccg tcggagacca ggtcgtcccc ttggatgaat atcgccgggc
    70801 ctatgaaaat acgtatcgct tctacaaaga caagggacag aacgacatca aggatgagtt
    70861 tctgaagcag tttgtgctgg agcaactcat cgacaatcgc atgtggctgc acgtggcgaa
    70921 agagatgggc ctgacggtct ccgacgaaga cttgcgcaaa gcgatcatgc aacgcacaga
    70981 gtttcagaag aacgggaact tcgatcccga ggcgtaccga cgattgctgt ccgcaaatcg
    71041 tttgaccccc gcttcattcg aggccatgga agccaaggat attctcacca acaaagcgcg
    71101 gctggtcatc atggatgctg tggcgctcac gccggctgaa tatacggaag cacagacact
    71161 cgcggctcgc gaaggggacg ccgatccggc caaggctgct gcggccaagg agcgggtttt
    71221 ccagagcctc ctgtttcaga agcagcaacg ggcgttgatg gcctacgcgg agtcgatgaa
    71281 gagcaaagtt cccgtgaaaa ttcacaaaga gcttatgtaa cggtcgccca gccactcccg
    71341 atcagcatcg catccccata actatagaat cgataccgtt gcgccactgc ctcttcatag
    71401 gcggcgcgga gcggttgcac acccacgaag gcggacacca gcatcagcag ggtcgtccgc
    71461 ggcaaatgaa agtttgtcac catcgcgtcg atcacctgaa attcaaaccc cggggtaata
    71521 aacagttcgg ccggtccctg aaagggctca atcccgcgcc cgcctcgcgc cgctgtctcc
    71581 agggcgcgca cgaccgtagt gcccactgcc accacacgat tcccccgctc ccgcgtcagg
    71641 cgaatctgcg ccaccgtctg cgccgacacc tcgattcgtt cggccagcat gcgatggtct
    71701 tcgattcgct cggtcttcac actgcgaaac gtgccggggc cgacgtgtaa cgtcacctgt
    71761 gccagctcga ttccctttcc cgccagggcc tgcaccaact cgcgcgtaaa atgtagcccg
    71821 gcggtgggcg ccgcaatagc cccttcatgt tgagcaaaca tggtctggta ccattcctga
    71881 tcgtccgctg acggttcccg tttgatatac ggaggaagcg gcatcgtgcc cgtggcctgc
    71941 atgagttcgt agacgcttgc gacacccgac acctgtacgg tcgttcgaag ccggctgcgc
    72001 tccacaatct ctccggtacc gccgcccgga aactcgatca ccgcgcctgg ccgaaagcgt
    72061 cccttgatca gcacctccca gagcccctgc ccgagatctt ccacaaacag caggtcgagg
    72121 agttttccgt tcgaccggac ctgcgcgggc acacgggccg gcatcacctt ggtattattc
    72181 accaccacga gatcccccga ctgcagcaat gatggaaggt cagccacctg ccggtgcacg
    72241 cgttcgcctc cctgccgatc cagcacaagc aaacgcgcgc gatcacgcgg gatcaccggg
    72301 tgatcggcta caagtactgg atcaaagggg aaatcgaact cgctgagtaa catcaggggg
    72361 tcggtatcac cggcatcatc aactgcgaga gcgccgcgtt gtgcaactcg gttccaggat
    72421 agtaatactg cagaatactg ctgtacgaat agcccagctc ggccaactct ttcgcgcccc
    72481 attggcagag ccccacggca tggcctgctc catagccggc aaacaccacc tcggcaccga
    72541 tcgattccac ctcgaactgg gtgctgggaa tgacggtgta ccccacggcc ttccgaagat
    72601 cctcgccacg caataccgtc tctccgccgg agtgcaaaat ccgcaggcgc gagacccgtc
    72661 cggcccggct gtaggcaatc ggagtgatcg tggcaatcgt tcccacagag aaaccctgat
    72721 gccgcagatt gtgctcaagt tgatcgatct tcacactggc ccgccattga tagtagggcg
    72781 attcgagatc gaatgggcat tcgaccccct tgaggtaggg caggtctttg ttcgcccaga
    72841 cattcacggc atcttccgtc gggccggccg cggtggatga gaacgcggca tagatcggcg
    72901 cctgttgatg ggtgacgaca atgcctcgcg tcgattcaac cgcatcctcc actcggcggt
    72961 ctactcctgt gcgacctcga tacacctgat cctgaatggt cgccaccaca tcatagtccc
    73021 gtgccgcact gagcattttg ttatacagcg cataggtccg tgccgccacg gcctgcacct
    73081 tcaacatttc cgggtgccag gccgaactga cctccgacgg cacgaccccc ttcacatact
    73141 cctccaaatc gacctggttg acaaccgaga gcccgtgccc tttgcgcgac agacgcacaa
    73201 ccccgctgag cgccaatgga gctcccgcgg ttgcgccccc ctctttgccg atcaccagcg
    73261 agaggttgtt ccgcagcgga cggatgagga cctgcccgcc catgctgcgc cggccatcga
    73321 catgcacgcc gtctcctcgg gccgtgagat ggatcggcgc ttgcaggatt ttcgtgtcgc
    73381 ccgtctccgt cacaagagcg agagcgccct ctgatcgcac ttccaccaac ggcgcctctt
    73441 gggccaagag gacgcgaatg gattcggcaa aggcggccgg tacgcagaac agcgcgagca
    73501 gcaacatgcc tcccgctgcg gccaggctgg atgactgatg tcgagtcagc ggcgaaagag
    73561 ccatgaaagg aagtagaaca ccaggctcag caggatgctg aacacgagac tggtcgcgat
    73621 cgggacatac agactgaatc gctctcgcgt gaccgagaag tcgcccggta atttaccgat
    73681 ccacgcgaac gcagagccaa ggcctgacca tttttccgcc agcgtgagca gcagcccgac
    73741 ggcgaccaac accaggcccg ccacaatcaa cgatcgaccg ataccagtcc acgctgccat
    73801 cgttcattgt atcagacatt cggcgatctg cacggcattg agggcggcgc ccttccgaag
    73861 gttgtcgctc acgacccaga ggttcaaccc attggtgatc gactcgtcca cacgaacccg
    73921 cccgacatac acgtcgtcct taccggacac gtcgaagggc atcggataca gtttcttgac
    73981 gggatcgtcg tacacaatca cgccaggcat ctccgccagc gcagcccgcg catcgttcgc
    74041 cttcaacgga cgttccagct caacgttgat cgcttcggag tgacagcgca acaccggcac
    74101 ccgcaccgta gtcgccgtga cccgcaacga cggcatctcc agaatcttcc tggtctcttg
    74161 aacgatcttg acctcttccg aacagtcacc gccgtcaccg aatgagccga cctgcggcaa
    74221 aaggttgaac gcgatctgat agggatagac ctgcaccttc acatcttgga aagccatcaa
    74281 ggccttagtc tgatcgacga gttcatccat ggccgccgac ccggtgcccg ataccgactg
    74341 aaaggtggtc accaccaccc gcttgatgcc tgcaatatcg cgaagcggct tcagtgccat
    74401 gaccaagggg gttgtggtgc agttcggaat ggccacaatc ccgcgcttca tcgagcgcaa
    74461 cgctgccgca ttgacctccg ggacgaccag cgggacatcg ggatccatcc ggaaaatcgc
    74521 gctgtcatcg atgacgacga ccccggccgc gcccaaccgc gccccgtagt cccgactgat
    74581 ggtatcggtc gcggacacga acgcgatgtc gactccggca aaggaagagg cctccgtcag
    74641 ttcctccacc ttgtactcct tgccctggca cgacaacacg tcgccggccg accgcttgga
    74701 ggagaacagg cgcaacgtct cgatcggaaa gttgcgttct tccagaatct ctagactttc
    74761 ttttcccacg gccccggtcg caccgagaat cgccaccgta taagcctgtt tcttcttcaa
    74821 catgatgctg tccttacgca gtcgcggtca gaaccctgat gcggtgattg aatgtatccg
    74881 caacatagac ggtcccggac gcgtccaccg ctacaccgaa tggataactg agacttcctg
    74941 ccaaggcatc gccgccatcg ccgccgaact gcgcgacacc ggtaccggac aagcgagaga
    75001 tcgtcttgct acgcccgttc cacacccgaa tcaagtgact gtctgaatcc gtcatataca
    75061 cgtttccatc agaaccgacg gcaatcccag caggcctgga gaggctcggc gaactgggct
    75121 cagccggtcc ctggtatcga taggtcccgc cgtctcccgc taccgtcgca atctgccccg
    75181 tcgccggatc gacagatcgt atccggctgt taaacgtatc agccacatag acgacgccgt
    75241 ctccgcctaa cgccacgccg ctgggtccgg ccagactcgc ttgaacggcc gaaacctggt
    75301 ctccgctata ggccgccgtt ccatctccgg ccaccgtggt gatgaccccg gtcgccaggt
    75361 cgacccgacg cacacggtta ttgctttgat ccgcgatgta caacgcctcg tcgctgaccg
    75421 ccaacccggt cggttcattg atcgcggccg acaccgccgg tccatcatcg ccggaatacc
    75481 gcgcctgacc tgtgccggcc acgttggtga tgatcccggt cgccgcatcg acctttctca
    75541 cccggtggtt cattgtgtcg gcgatgtaga ggttgcctgc gcgatcgacg gctactgcac
    75601 tggggaaatt gagtcgcgct cgcctggcag ggccgccgtc gcctgaatcg tgctcgaccg
    75661 tcaaacgccc tccggtcaca taccggacgg tgccactcag atcggtcacc tgcgtgtagg
    75721 ccttggtact atcaccggaa tggtcggcaa agggatcctc gtcttcctca gcctgcgccg
    75781 ccacaggcgg ttcaggcact gccggtccgg tcgctcctga cggacaaact ccggccacgg
    75841 tcgtaatgat gcctgtgacc cgatcaactc gacgcacaac atggttttcg gaatcggcaa
    75901 tatagaggtt gccctcccga tcaacgcaca agcctttcgg ctcgttgagt gaggcggcac
    75961 ccgcaggccc accgtcaccg gcgtaccccg gctcgccgtt gcctgccaga gtctcaatga
    76021 ttccggattg tgtcgtgcgt gtcgtcataa cttacgaggg aaggttgcgc acaatcgcat
    76081 cacccatctc aacggtgccg accagctgca tgccttcggc atgaatatcc ttggtgcgga
    76141 acccgagttc cagcgtcttg acgatggcct gctcgatcgc cgctgcttcc ttgtcgagct
    76201 gaaacgcata ggacaacatc attgaggcag aagcgatcgt tgcaatcgga ttggcgatgt
    76261 ttttgccggc aatatccggc gcactgccat ggatcggctc gaacaacccc accttggcgc
    76321 cgacactggc ggacggcaac atgccgatcg acccggtgag catcgcggct tcgtcgctga
    76381 gaatatcccc gaagatgttg ttgcacagca acacatcgaa ctgccgcgga ttacgcacca
    76441 gctgcatggc gcaattgtcg acatagatgt gactcaaggc cacgtcggga taccctttgt
    76501 gaacgtccgt gaccacccgc cgccacaact ccgaggactc cagcacattg gccttatcca
    76561 ccgaggtgac ctttttacgg cgcttgcgag ccgcgtcaaa cgccaccacg gcaatgcgac
    76621 ggatttcctc ggtcgtgtag acctccgtat tgaagccacg ctctccgccg cccggcagct
    76681 tttcaatccc cttcggcttg ccgaaataaa tccctccggt cagctcccgg atcacgagca
    76741 tatcgatgcc ctcaatcacg tcacgtcgta acgtggaggc gtcggcaagc atgggataga
    76801 gcttggccgg tcgaaggttg gcatagagcc cgagttgctc ccgcagcccc agcaaggccc
    76861 gctcaggacg aaggctgtat tccagacctt cccatttggg cccgccgacg gcgccgagca
    76921 acacggcatc gctgtgcttt gcgatggcga gcgtctcagc cggaagcggc acgccgactt
    76981 tatcgatcgc atggccgccg acatcgccgg atataaagtc aaacgtatgc ccgtagcgtt
    77041 ccgcaacgac cttcaacacc ttgaccgctt ccggaacaat ttcacggccg actccatctc
    77101 cggccaggac tgcgattttc gcgttcacgt gcagatctcc tctcgactat ccaccaacgg
    77161 cctcatcctc cgcggtccag gctggatcga ccaggcagag aaactcaatc ggggctgttc
    77221 cattgttcac taacgattgc ttggcccccg ggggcacgta gatcacggag cccgcctcaa
    77281 ccgcaaccga ttcgtcctcc accttcatca ccccccggcc ggcaatgaag taatacactt
    77341 cggaagattt taagcgatgc cagaggctct gctgacccgg ggcgagcagt ccgtgcgc
//
LOCUS       DAORRQ010000014        75968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_15_length_75968_cov_132.160974, whole genome shotgun sequence.
ACCESSION   DAORRQ010000014 DAORRQ010000000
VERSION     DAORRQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 75968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75968
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15_length_75968_cov_132.160974"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PK224_06370"
     CDS             <1..236
                     /locus_tag="PK224_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRB15396.1"
                     /translation="DRLEDKELIRRTPVADDRRSTRICLTEKGDRLFKKTFAAHIAFL
                     RPFFERALTTAEAEQLRTLLRRLHRSFQEKPTA"
     gene            233..991
                     /locus_tag="PK224_06375"
     CDS             233..991
                     /locus_tag="PK224_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB15397.1"
                     /translation="MIRVTVLGSGTNVHPTRAAAGYLVQTDQTFLLDFGPRTLSNLIT
                     SGVDRHRITHILFSHFHADHFADFIPFFFDAVIFCKYQGGTRPPLTLIGPHGTAKLMR
                     AMMTTFPSFNRAPFRVTIREVSDRSFRLGETRIKPGTVTHAPRLHCVGYRIEYQDHVV
                     TYSGDSLYCDNLVRLCHEADLAILDCSFPENRPGAGHLHAGLCGRVAQEAGINQLVLS
                     HFYPIAERYDVCAQAGNYFSGRIRMARDLLKLKA"
     gene            complement(1181..2041)
                     /locus_tag="PK224_06380"
     CDS             complement(1181..2041)
                     /locus_tag="PK224_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRB15398.1"
                     /translation="MFVNESQIRALIRLLGDEDERIVKTISGKLIDFGDSAVPLLQEA
                     EIEQPEMADRIVTVLEEIRGTRLEEELRDLIALPEDHIDLETGAFLLARYAYPTLDVQ
                     SYQRQLDRMAQDVREQIGSRVSGEETVKALNRYLFTEAGFRGNTKNYYEVENSYLNCV
                     MDRRTGIPISLSTVYLLIGKRLHLPVQGIGMPGHFLVKFDSDRYKIFIDCFNGGALLT
                     EKNCARFLTEAGYGFEDRFLQKSPTRAILARMIKNLLAIYNKLDEPLKKTRLPRFIEI
                     LGCEQREEGL"
     gene            complement(2068..2847)
                     /locus_tag="PK224_06385"
     CDS             complement(2068..2847)
                     /locus_tag="PK224_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB15399.1"
                     /translation="MKEYWQEIRALTMRWVRRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADMQVTQASSYISFLTAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     TSVILSRFIFVTTITSLQVLVILGVAWLFGVQPVTGLLGVATILFIGMMFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAAMPGWMGFLAQFNPMTWAIDAVR
                     PLILQGWAEALPHVGMVIGVMVLFDALCLYGGARAFRRAIG"
     gene            complement(2877..3848)
                     /locus_tag="PK224_06390"
     CDS             complement(2877..3848)
                     /locus_tag="PK224_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB15400.1"
                     /translation="MSRAVAIEVSHLGKTYEKVRAVDDLSFQVYAGEIFGLLGPNGAG
                     KSTTLRILITLLHPTSGSATILGMDSVKDADRVRKTIGYVPQERAIDRFLTGREHLEL
                     LADLYHLSPEDASTRIPQLLKLVELEQQADRPAKTYSGGMKRKLDIACGLLPDPKILF
                     LDEPTLGLDVQSRLRIWDHVRAMRERGITVVMTTNYLDEADQLCDRIAIIDGGRIKAL
                     GVPNELKAGLGGDLVSLTVREHDRVELLAAAVKNLPAIRAVTTTPTGLDIRVDSPEKA
                     LPAILEAANRLTCQLEFIDYHRPRLDDVFIAHTGRSIKDDQPKAEGQ"
     gene            complement(3848..4537)
                     /locus_tag="PK224_06395"
     CDS             complement(3848..4537)
                     /locus_tag="PK224_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRB15401.1"
                     /translation="MFLEPERDQSSRELSGKAQPFPVPERIPLFPLPNLVFFPKTYLP
                     LHVFEPRYRQMVADAAAGGQCIGMALLKEGWEEQYDGNPPIFSIGCVGRLASVQALPD
                     GRSNILLQGLERYEIHEEFYEKSYREARVALKPREGAVSMEPALRRYLTDVLDEYLKA
                     DEEASPLHSLVRPDVSDEVFVNSLSTYLDCTPLEKQFLLEADHVPQQARRLSDLIQFK
                     LAERHGAGGWG"
     gene            4717..5856
                     /locus_tag="PK224_06400"
     CDS             4717..5856
                     /locus_tag="PK224_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HRB15402.1"
                     /translation="MITVRHSAFAALAVMVVLLTGTVHSVATDNQAPSFDTAKPGYRY
                     EFPKDHGAHERFRTEWWYYTGHLTAAGGRQFGFELTFFRRGIPPEQVRTQPSQWSIQQ
                     LYLAHFALTDLDGNRFLFADKLSREGLGKAGAETDRLHVWLDRWSATMDDPTSSRQKL
                     QAATDAFAIDLQVTPGKPPVLHGREGISRKGARPEQASHYYSFTHLTTEGEIRIGADT
                     FSVSGLSWMDHEFGSADLGRDVVGWDWFSLQLSDSTELMWYSLRRADGSADPVSSGTL
                     VFADGRTQPLTAQDLTVESLDYWTSPRSKAQYPQQWRITAPSLGLNLDVRSLLADQEL
                     NTARSTRVMYWEGAVSATGQVHGTAVTGRGYVELTGYAERFTQRL"
     gene            5938..6969
                     /locus_tag="PK224_06405"
     CDS             5938..6969
                     /locus_tag="PK224_06405"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15403.1"
                     /translation="MAALLRTSVCLVGLFLLACGSREAAVVSIALHPSNPNIVYVATN
                     EAVHKTRDGGATWEQFQAFTARRVTTLAIDPKLPATVYAGTMGDAIYKSPDGGQHWLP
                     HNVGLKEHVSFVNEIIFHPDNTELIYLATTVGAFVSKNGGREWEERMAGMKEVHIVTC
                     ITMDHQHPRVLYAGTTGGTYRSEDGGTTWQKKNNGLIPDDVLNAAMALGVNMLAVDPR
                     NSDIVYAATTKGLFRSTNRAGYWEQIGQNLANQFISTLALHPTDPKIVYIGGPEGILK
                     TVDGGKTWQAHNQGLTTMNVRTISLSPSDPQLLYLGTNGSGLYRSTNGGDQWTPIPLT
                     SHAQAAPAP"
     gene            6988..7596
                     /locus_tag="PK224_06410"
     CDS             6988..7596
                     /locus_tag="PK224_06410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRB15404.1"
                     /translation="MPIVLLIRHGETEWNRSGRVMGDQPIPLNRTGEEQARTCAGILS
                     RTPIAAIYTSPVLRAAQTAEILRGPQEIPLHQVPGLSEIGVGNWINRYWHEFADDPAK
                     REWYTHPDRARPSGGETLREVQQRAVAAVEQALAPAHDSPVVIVSHGDVIRAILAHYL
                     QLDLAIIRQARIDHLAVSGLDLTGTAAQLLFLNHRPGLETLL"
     gene            complement(7613..8104)
                     /locus_tag="PK224_06415"
     CDS             complement(7613..8104)
                     /locus_tag="PK224_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15405.1"
                     /translation="MRYRSLVVLIGVLVTGLTGCSSHHHGSYGHGKGDYYWSKASQDV
                     NGLVEKHVKNPETAKQVNTVMGEMITEIKSAREQHRQSHRALYELNANYAATPEEFTK
                     ILDELNNNRMRSSARMLSLRFKMKSLLTAEEWSALSEELKRYGSRYYGKGAEAAAPAA
                     AQP"
     gene            8215..8571
                     /locus_tag="PK224_06420"
     CDS             8215..8571
                     /locus_tag="PK224_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15406.1"
                     /translation="MGTQKDHRQGTGSVESLRDEFREHLHIFYSTLKLAAPYDTVEKA
                     LGALTARLKTLSPEELQQISTDSSLRWTCFRQAFVESGLSAKHRGIIAGLARSGAALD
                     LPPEFEPLLNLYRPTS"
     gene            complement(8706..9326)
                     /locus_tag="PK224_06425"
     CDS             complement(8706..9326)
                     /locus_tag="PK224_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15407.1"
                     /translation="MILAGAFAIVLFVGLIVGDWMYFARLSSDASRYGYGIGRVQDHF
                     AALTVPQLLSTFDQNGFLSLPHGVARVFPEANRMIIRPTHRLFSLRFRTAWPLKGSIE
                     WAAESEGLKMVCTKRAPWSSSILTFLWFSLVGFGTLGFVITYLLQGGFDSLGSVLLGI
                     GVTGVGMIVVAFGLLTITVAYRIENTRLMQVYTELRDVALKSEGAA"
     gene            9580..10272
                     /locus_tag="PK224_06430"
     CDS             9580..10272
                     /locus_tag="PK224_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB15408.1"
                     /translation="MDIAKVERVYTSYSGVYDHIFGKIFHESREACVRNLRIRPEEKI
                     LEVGVGTGIALEYYPKNCEIIGIDLSSGMLAKARQRQSHYHLDHVRLMLMDAGKMDFA
                     DDSFDTVMAAYVVTAVPDYRKVVNEMIRVCKPGGRIIMLNHFSNGNKLIAAVEKVISP
                     LCKHIGFRTDLSLNTVLEGTNLHVARKEKVNPMKFWHLVECVNQKGAKNGSVNGHGSA
                     NGNGNGRHGHHS"
     gene            complement(10294..11112)
                     /locus_tag="PK224_06435"
     CDS             complement(10294..11112)
                     /locus_tag="PK224_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15409.1"
                     /translation="MTHSIKTVDDLRWFLARTAGFRGGHITDVHLSKRRIFDEASGRE
                     VMAGSMATVVVRYHTQGILRVAKLTMLGVTDLSLFEQDGSDCSSLGAIQVEMSEGKLR
                     FWFDPDGNLYTVCEEATLEEVSLPASESDMGGVIAQWTFQADSGVAPTVEWLLARLDQ
                     VGVPCIWKAATRRQGRHSLMCWEGELAMAADMAAGAAAALRVEAYGPLDGAGFGLRLQ
                     ARDAAGRLGGRLLSLVTDLVTQRYRGTCLVGQTFLSHEEWVKWNSFRPTHQASG"
     gene            complement(11242..11445)
                     /locus_tag="PK224_06440"
     CDS             complement(11242..11445)
                     /locus_tag="PK224_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15410.1"
                     /translation="MGSGFEDGQQAQLELAGLRRSLKWTNIQREQLLDRLDLLRLDNQ
                     RLQERVDELERQLAEFKQQQALF"
     gene            complement(11598..12899)
                     /locus_tag="PK224_06445"
     CDS             complement(11598..12899)
                     /locus_tag="PK224_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB15411.1"
                     /translation="MSDIAIKADGLSKHYRLGAASRQHNTLRDHLMHRLRGLTRLGGG
                     PPESNPSFWALKDVSFEVKRGEVLGIVGHNGAGKSTLLKILSRITQPTTGTADIYGRV
                     SSLLEVGTGFHSELSGRENIYLNAAMLGMRRGEVRRKFDEIVAFSGVEAFIDTPVKRY
                     SSGMYVRLAFAVAAHLEPEILIVDEVLAVGDASFQQKCLGKMEEVSRSGRTVLIVSHN
                     MTVIEGLCERAILLEKGRVAKIGNTHEVVEGYADAIRGLAGTAIEARHDREGLGEILL
                     TRIEVMDTQRHPVAAAITGRDVVIRMYYRCAAGKEFLNCRVSISVNGRKAQDLFVLST
                     DIVDPKPLTLRREGYVDFILPELPLTGGAYFFQSYIESNGHAQDWIKNVAPFSVLDAD
                     YYGTGKLCPPGWEANGVLVRYRWEAGETRSTNPLPIRARAS"
     gene            complement(12900..13757)
                     /locus_tag="PK224_06450"
     CDS             complement(12900..13757)
                     /locus_tag="PK224_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB15412.1"
                     /translation="MSALTADQRPLSTLPHVRIQPARGLLDLDLAALWQYRELIYILV
                     WRDVTVRYKQTLLGVAWAMFQPVATMLIFTVVFSVMGKIPSDGFPYPVFLYAGLLPWF
                     YVSQALSRGGTSLVGEAPLISKVYFPRLILPLSATIAPLVDLALAFVAFLGLMAWFGI
                     LPTWRILSLPLFASLAATITLAAGLWISALYVKYRDVGHILPMLIQLWMFVSPILYPV
                     SKVPESWRALYAMNPVVSVVEGFRWALIPGFQVDFTMFLPSLGVVFVVFVGGLIYFRS
                     MERTFADVI"
     gene            complement(14136..14681)
                     /locus_tag="PK224_06455"
     CDS             complement(14136..14681)
                     /locus_tag="PK224_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15413.1"
                     /translation="MNIRNRAMMKQASRLALVLVLGLSSACVSVQVDPLTSKAFEPRP
                     GSEDVTTLQREPHHGHVQIARIIATSEYASEDTLRDRILARAKELGADAVVLGEADVR
                     RLADRGPTFQSTMGTAVPGATSGNSYRSGYWNPFRMDAWSFTQGAGGGQGWMLHMSGL
                     AIRYVSADELRAAPKPVPPAP"
     gene            complement(14850..15209)
                     /locus_tag="PK224_06460"
     CDS             complement(14850..15209)
                     /locus_tag="PK224_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HRB15414.1"
                     /translation="MIRSLQPCPSSPNCVSTQASDHGHGIVPYRYQKTLAGAKETLKT
                     IVANLPRAKLVEEDEAYLHYEFTSLLLRFVDDVEFVFDDATKTIHFRSASRTGYSDLG
                     VNRRRMEEIRVVVEGRL"
     gene            complement(15388..16572)
                     /locus_tag="PK224_06465"
     CDS             complement(15388..16572)
                     /locus_tag="PK224_06465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRB15415.1"
                     /translation="MVSSNNMDATSLWPAVAALLLAVGTATLLPDIGHLRTTSAARYS
                     CIDGLRGYLAFAVFLSHSSVWYFYLRSGTWDVPPSNFYTHLGQSSVTLFFMITGFLFW
                     SKLLDGRVQPVDWSRLYLSRIFRLVPLYLLMVIGVVSIALYRAGFRLQESMTSFALHL
                     ASWLTFTIPGIAPVNGFAETIPLAGAVWSLPYEWLFYAFLPLGSWCLRAATPTSWLLF
                     SAAAVVTLTTVMPDVRGPVLYAFGGGIAAASLARVDAIRTRLAHRVWGLVVVACLGIT
                     MWLFPTAYTFPALMLLTGAFTIIACGNSIYGILEWPTSVLLSEMGYSLYLLHSLLLFA
                     AYRLVLGEWASTLSAAEHWSIVLGLVPVLILLCFATFRLIEQPAMAAVPRCHAWLVAH
                     LN"
     gene            16824..17999
                     /locus_tag="PK224_06470"
     CDS             16824..17999
                     /locus_tag="PK224_06470"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRB15416.1"
                     /translation="MAGFPIEVATRIKTLPPYLFAAIDKMKQEALARGVDIINLGIGD
                     PDLPTPTPIIDSLAQAAKNPKHHQYPSYEGMLSFRKAVADWYKRRFNVALDPADEVLT
                     LIGSKEGIGHVHLAFIDPGDIVLVPSPGYPVYPVGTSFCGGVSHIMPLTKANGFLPDL
                     NAIPKEVAKKAKLMWLNSPNNPTSVIMSKDYFKRVVEFAQDNQVIVCHDAAYSEIYYD
                     GKRPASFLEVDGAKDVGVEFHSLSKTYNMTGWRIGFAVGNKGVLAGLGRVKSQLDSGV
                     FEAVQAAGITALGLDDSVTDGLRKIYQERRDTLVPGLKKLGLEVDPPPAAFYIWVTVP
                     KGYTSASFTAHLLEKAGIVTTPGNGFGAPGEGYIRMTVCTTKERLAEAVERIKKAGF"
     gene            18087..18650
                     /gene="folK"
                     /locus_tag="PK224_06475"
     CDS             18087..18650
                     /gene="folK"
                     /locus_tag="PK224_06475"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRB15417.1"
                     /translation="MATAFIGFGSNLGNRIDFCDRAVTLLGLLPHSRLEAVSSLYETE
                     PLEDGAAPGPSWFLNGVVQIETNIAPKSLLEICREIERALGRDQDNRKGPRTLDLDVL
                     LYDDCILAEPALTVPHPRLHQRRFVLTPLVELDPERRHPVLGQNLRDLLSQLTDPSIV
                     RLLEPQPNSRYGSRPTCSAPPTAGPQA"
     gene            18647..19504
                     /gene="panC"
                     /locus_tag="PK224_06480"
     CDS             18647..19504
                     /gene="panC"
                     /locus_tag="PK224_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRB15418.1"
                     /translation="MKIIRATPAMADWSRRLHREGITIGFVPTMGALHEGHRALIRAA
                     RLACDAVVVSIFVNPTQFGPREDLSKYPRQLRRDQALCRKEGVDVIFAPTVRTMYPTG
                     AQTVVTVPGIARRWEGEARPHHFQGVATVVTKLLSLVRPDKTWFGQKDYQQAALVQQL
                     VKDLNLPGLPIVHPTVREADGLALSSRNVYLSSSERQAAPVLSRALQAGVAAIRAGER
                     NGTRIRRHMLRVVATEPLATVEYLAVCDPATLEPLSLLNRQAVLLGAIRIGTVRLLDN
                     VLTSFRRKP"
     gene            complement(19590..20099)
                     /locus_tag="PK224_06485"
     CDS             complement(19590..20099)
                     /locus_tag="PK224_06485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB15419.1"
                     /translation="MKELYCPACGTPCVRVTSGTNLMEKTLNRLSIFQVRCQLCTARF
                     QARRPGNRQTSQEFDRREYRRLRANFAASLILDQPAVGGVITDISMGGCTLQASSSLP
                     RGTFVKLIVHAPAGQPDIKVDAAMICSIQPLSIGVKFLEFEPDDKQRMGQLVLELLAH
                     QQAPPRVSA"
     gene            complement(20101..20529)
                     /locus_tag="PK224_06490"
     CDS             complement(20101..20529)
                     /locus_tag="PK224_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRB15420.1"
                     /translation="MMDPQAMVEEFHRKFDIAVSDRPSLPEEATRQLRVRLIQEEFEE
                     LQEAMVAQDLPGVAKELADLLYVVYGTAVSYGLDMDPVFREVHRSNLSKVGGYKRADG
                     KWVKPPTYSPAQVKPLLAVQSSGSLADQSQVDTRMQESES"
     gene            complement(20526..21941)
                     /gene="rmuC"
                     /locus_tag="PK224_06495"
     CDS             complement(20526..21941)
                     /gene="rmuC"
                     /locus_tag="PK224_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRB15421.1"
                     /translation="MIDLTSPTFLVGTAVGLAVGVLVAGWWVIVRVQSRHHARLLESG
                     ERAQRADAVAASLQQRVDDQQSEMGQLRDALAESQQGRTKAETRMEEVARSLEDQKAL
                     LAQARQELHDSFEALSGQALKQNNEAFLDLARTSFATLQAEAKGELSQRQQAIGELVK
                     PLEESLQRYQEQLQQAEQVRQREYGGLDQQLRFMAESHQRLQQETGNLVKALRAPSVR
                     GRWGEMTLRRVAELAGMVAHCDFVEQDTIGSLEGLIRPDMVVYLPGGRQIVVDAKTVL
                     AAYLDAYEAEDDRQRQAHLLRHAGQVRARMDALSLKAYWTQFGQTPEFVVLFLPGEQF
                     LGAALEQDPTLIEDGFAQGVVLATPTTLMALLRAVAYGWKQEQLNEHAEQAGRLGKEL
                     YERMAVLTDHLNEIGQALGKSVGAYNKAVGSLESRVLPAARKFKDLGISSDKDIALLE
                     ASGIEPRTGVAFAAESRGTLG"
     gene            complement(22037..24667)
                     /locus_tag="PK224_06500"
     CDS             complement(22037..24667)
                     /locus_tag="PK224_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRB15422.1"
                     /translation="MADPFLLSLYESCAPFLVGGKAAGLAKVLIAGLPVPKGLCVTTA
                     VYQHGLDQAGVDAVALWTKLPHLSEGQRAQELARIQRLLLEQPWPTGFPADLETRLTG
                     LAGDSSTRWAVRSSATNEDAADMSAAGLYRTTLGVPLAAVLQSIRDCWISLWDERVFR
                     YFLRSGADRPCPAMAVVIQPMLSAQAAGVAYSIHPVTGRTSQVSIDAVPGLASSLVNG
                     VVTPDQYVVEVYDDDHRPFRVRRRMLARKRNKLTATPGGVREESIPEPEQRQACLTDK
                     QLFELARLSKRIEVAFRQPVDLEWVLDVERLWIVQARPITAVRPWPSLTNDECEWTRA
                     NFKETLPELPSPLGLSFLERFMDAYIITPYRRLGCTVPEGLSSVRVFHGRPYLNVTLF
                     YTLVMQLHGNPAFLTEQMGGEPLMFTPSVRPLGALALLRAGVGMLREWRKAAKQGPKN
                     FSAMKAMAQRYRYDRIQNLSVQELAAILDSIGRWLDDHEVTFAIAGGVAQSLQAMGTV
                     LPGWLGSDWRELLNGALQGQGTVISASQIVRLAELVVAAQQEETVRQWFFSEEWAACG
                     YRDAIQGTEFLRLFDQYLAEYGHRAVGESDIMSPRIADQPDAVLALLRAQVRAGVTAP
                     PQEVLSRQAQRREQALSEIARRFGWRRHRWLVFRWWYRRLSRFCALREENRHHLMYYS
                     TAARHLLLRLGELMVARGSFAVREDVFYLTLDERIALTDGASRDWQSLVRRRREERLQ
                     HEALQVPDTIRDWEAVVEQGAQSSDQGQGGVLRGIAISAGVAKGPVRLVRSTADWARV
                     RAGDILVVPVIDPGMAPLFGMAAGLIAEMGGTLSHGAIIAREYGLPVLVNVPYATSLL
                     RENEQVEIASSTGRISRLVS"
     gene            complement(24683..26023)
                     /locus_tag="PK224_06505"
     CDS             complement(24683..26023)
                     /locus_tag="PK224_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRB15423.1"
                     /translation="MIQVGVSQLRARALFTTLFRAGLQAVDPYEAVCRQVRFRRGQLT
                     IGSERYPLSQHQRLVVVGAGKASGRMAQALERQLGARIDTGLVVVKYGHGAPTKKIRI
                     LEAGHPVPDEAGLQASRAMMALIGTLKSDDLLIVLLSGGASSLLPSPAPGVTLKAKQL
                     TTKLLLRSGATIHEMNAVRKHLSCVKGGQLAAATSARVASVILSDVIGNDLGTIGSGP
                     TAPDPTTFRDAWRILERYEVARKVPASVRRRLEAGMKKTLPETPKAGAVLFRRVDNLL
                     IGDNQAAVDAVVKAAKGKRLQTLVLSTTVTGEARELAKFFGAIAREIATHGRPLARPC
                     CVIAGGEPTVTIRGQGKGGRAQEFALAAALEIAGLSDVWVAGFATDGTDGPTSVAGAV
                     VDGETTARARRAKLDPPDALQGNDAYPFFKKLRSHIVTGPTGTNVNDLYLLLAL"
     gene            complement(26029..27108)
                     /locus_tag="PK224_06510"
     CDS             complement(26029..27108)
                     /locus_tag="PK224_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRB15424.1"
                     /translation="MWNKERGVQAFLACVTMLFGLSSAMPVLSADSSSAALTGAKAEE
                     GRNAQESDQVPVAPPGESELVPELEDRLVILPEIKREGERFFLSSFKLPDKLTFAGQA
                     IPLDNWQVRERIEYEFYQFLEDQGESIILAKRTGRCFAPAEKQLAEAGLPDDLKYMLL
                     VESKCIAAAYSRAKASGPWQFINSTGRRYKLHNERWLDERRNLEMSTEAAIKYLRYLR
                     DLYQGDWFLAMASYNAGEDRVRKLIKEQKINDYWRMHGPRETMRYVARIIAAKEIYSQ
                     PEKYLGLTKKDLYPPLETETVTVTIKEPQRRLTAIAEEYGTYFLELKMLNPEFTKDYL
                     PKGTYQLKVPRQTCPNRCFKQEKLP"
     gene            27204..27902
                     /locus_tag="PK224_06515"
     CDS             27204..27902
                     /locus_tag="PK224_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRB15425.1"
                     /translation="MSALTMLNNEIVACTCCPRLVAYREAVARDKRRQFREWSYWGKP
                     VPGFGDSNARLYILGLAPAAHGGNRTGRVFTGDRSGDWLYEALHRFGFANQATSVHAG
                     DGLTLTDCYIGATVRCAPPANKPTPDEFTACRPFVLRELRLLKRMRVIVTLGKIAFDH
                     YLKASRELGLGTPGLRPQFGHGVVYDLPWGVTLIGSYHPSQQNTFTGKLTRPMFHRIF
                     LKAQQKLAEAPLRD"
     gene            complement(27916..28563)
                     /gene="fsa"
                     /locus_tag="PK224_06520"
     CDS             complement(27916..28563)
                     /gene="fsa"
                     /locus_tag="PK224_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRB15426.1"
                     /translation="MQIYLDTANVKEIQEGANLGLIDGVTTNPSLVAKEGRSFKEMLL
                     EICKMVDGPISAEVVGVESEAMIKEGRDLAKVHKNIVVKVPLIPEGLRATKKLAAEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDVSSDGMALIRQIVTIYKNYDYKTQV
                     LVASVRHPQHVVEAALAGGHICTMPYAVFQQLVKHPLTDSGLKKFLADWDAMGKK"
     gene            28762..29064
                     /locus_tag="PK224_06525"
     CDS             28762..29064
                     /locus_tag="PK224_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15427.1"
                     /translation="MRKSAWLIAIALLSGACSHNPYLDASLTRYQGKDRAWIEKELGA
                     PDAKTSRFFGGEKWTYNRIAGGKAGPPLFNFKANECQLILFFNKDEMVSDSSYSGC"
     gene            complement(29061..29321)
                     /locus_tag="PK224_06530"
     CDS             complement(29061..29321)
                     /locus_tag="PK224_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRB15428.1"
                     /translation="MYRLLLVSGLLVLLYYLLRRAIRKMKEDGGAVSLQEEQAAGNQM
                     IQDPVCRVFIPRGNAVREEIGGQTYYFCSRSCADTFQKQLSS"
     gene            complement(30014..30817)
                     /gene="dapB"
                     /locus_tag="PK224_06535"
     CDS             complement(30014..30817)
                     /gene="dapB"
                     /locus_tag="PK224_06535"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRB15429.1"
                     /translation="MIKVVITGAAGRMGSRLVSLVKDSAFLTLAGAVEGKGHHAVGED
                     SGEVAGCGRTGVPIVDDLSSLMERGEVVVDFTTPAATLGHLKIVAQHRRGIVVGTTGF
                     SSSELDELRSVSKQIPCVFSPNMSVGVNVIYKVIAEMAKTLGEDYDIEVIEAHHRLKK
                     DAPSGTALKMAEVLARAVNRDLGQVGVYARKGLIGERKRGEIGIQTIRAGDIVGDHTV
                     IFGTMGERIELTHRASSRDTFARGALRAARWVVKQPPGLYDMLDVLSLK"
     gene            complement(30847..31719)
                     /gene="dapA"
                     /locus_tag="PK224_06540"
     CDS             complement(30847..31719)
                     /gene="dapA"
                     /locus_tag="PK224_06540"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRB15430.1"
                     /translation="MFTGSLVAIVTPFKNGKLDEKSLGDLIEWQITSGTQGIVPCGTT
                     GESATLTHAEHDRVVAFTVEVARRRVPVIAGTGSNSTQEAIALTKHAKAAGAGGALLI
                     TPYYNKPTQEGLFLHYKAVAEAVDLPLVLYNIPGRTGVNMMPSTISRLTVCPTIVAIK
                     EGSGSVQQASEIIQLCGERLTVLAGDDALTLPMMAVGGKGVITVTANLVPSDMAELVN
                     AFLAGRVDDARAMHYRLYPLFTALFYETNPIPVKEALHMMGKIDREMRLPLCPMGTDN
                     REKLLHVMKEAGLV"
     gene            complement(31756..33018)
                     /gene="lysA"
                     /locus_tag="PK224_06545"
     CDS             complement(31756..33018)
                     /gene="lysA"
                     /locus_tag="PK224_06545"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRB15431.1"
                     /translation="MNDFQYREGELYCEDVPLSRIAKEVGTPCYVYSHHTLVRHFRVY
                     DSAFQNIPHIVAFAMKANSNLAVLRLMAKEGSGVDIVSGGELFRALKAGVSPAKIVFA
                     GVGKKAEEIRDALKADILMFNVESSAELQAINDVAASMGVKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRAIEEFKAAATFSHIEVVGLHAHIGSQLTQVAPFVESLKKVL
                     AMVQTLAEQGIPIRYLNIGGGLGITYSDETPPEPKDLAAAIFPLVRDLKCILIMEPGR
                     VIVGNAGVLLTKVLYTKDGETKRFLIVDAAMNDLIRPSLYDAHHDIRPVYENVARAAK
                     QTVDVVGPVCESGDFLAKDRVMPEMNAGDLMAVMSAGAYGFVMSSNYNSRPRVPEVLV
                     HEGQIHIIRARESYDDLVHGEQIPAFLS"
     gene            complement(33420..34874)
                     /gene="argH"
                     /locus_tag="PK224_06550"
     CDS             complement(33420..34874)
                     /gene="argH"
                     /locus_tag="PK224_06550"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRB15432.1"
                     /translation="MAKPQSAGARARKSVTAPQRSRRALPKGKAWGGRFAEQTDRLVE
                     QFTSSLACDRRLYPYDIQGSIAHCRTLERAGVLTARESAQLVRGLQFVKVELDGGRFP
                     FSPQDEDIHMAIERRLTELIGPLGGKLHTGRSRNDQVALDLRLFLRDVLSTLMGQLQE
                     FRRVLVGQARAHLDVVMPGYTHLQRAQPVLLAHHFLAYVEMFDRDRGRLHDCRQRLDV
                     MPLGSGALAGSNYPVDRRYTAALLEFPAVTQNSLDAVSDRDAVVETLSALSLIMMHLS
                     RLSEELILWASQEFRYVDLPDTFCTGSSMMPQKKNPDVPELVRGKTGRVYGHLMGTLT
                     LLKGLPLSYNRDLQEDKEALFDAVDTTGQSLALCTELMRRLVVNKTVLAEAAEGGGML
                     ATELADYLVTKGVPFREAHSITGQIVRFSLEQQRPLQQLTLKDLRGFSAHFGEDVLSC
                     LTVRGAIDRKGQIGGTARRRVEARIKELEKALKG"
     gene            complement(34874..36091)
                     /locus_tag="PK224_06555"
     CDS             complement(34874..36091)
                     /locus_tag="PK224_06555"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRB15433.1"
                     /translation="MSRSSYKKVVLAYSGGLDTSVILKWLEEVYGCEVIAFCADLGQG
                     EDLKAIKKKAQSLGVKKVYVEDLREVFVKDHVFPMLRGNAIYEGSYLLGTSIARPLIA
                     RRQIEIAADEGAEAVCHGATGKGNDQVRFELTYMALHPQIKIIAPWREWTMRSRRELI
                     EYAEKHGIPVTATKAKPYSMDMNLFHTSYEGGILEDPWKAPPEEIFVMSVSPERAPNK
                     AREVEIDYVAGNPVAVDGKKMSPAALLAHLNKLGGEHGVGRVDLVENRYVGMKSRGVY
                     ETPGGTILHAAHRGLESLTMDREVLHLRDSLIPRYAELIYYGYWYAPEREMLQVALDE
                     AQRDVTGTVRVKLYKGTCTVVGRKSPRSLYRLDMATFEEDDVYRQKDAEGFIRLNALR
                     LAIRAQRKKRSTR"
     gene            complement(36103..37086)
                     /gene="argF"
                     /locus_tag="PK224_06560"
     CDS             complement(36103..37086)
                     /gene="argF"
                     /locus_tag="PK224_06560"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRB15434.1"
                     /translation="MSRRPHRSSSRAGLGKDFLELLSIPVAELTGLLRLAAQLKVKQR
                     RGVSHPLLQGRMLGLLFQKPSTRTRVSFEAGMNQLGGQAMVLPMGDIQLSRGESVADT
                     AHVLSRYLDAIVLRTFDHAIAEEWAREASIPVINGLTDLNHPCQALSDLLTIQEKKRR
                     LKGIKIAYVGDGNNVTNSLIEAAAKMGMTIAVGCPLGYQPDRGIVDRAREEARQTGAV
                     IEIGADPFVAVKDADVVYTDVWISMGQEREQAKRLKILAPYQLNARLLKCAKPDALVM
                     HCLPAHRGEEISAEVLDGPQSVVFDQAENRLHMQKAILVRLLGRNLHGARK"
     gene            complement(37103..38311)
                     /locus_tag="PK224_06565"
     CDS             complement(37103..38311)
                     /locus_tag="PK224_06565"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HRB15435.1"
                     /translation="MPTGELRLNAEQYLMNTYTRQPISIVRGRGSKVYDLEGREYIDF
                     VAGIAVNLLGHGHPDLVLAIQKQVQHLIHTSNLYYTEPQVRLAQTLVEHSFAQKVFFC
                     NSGAEANEAAIKLARKYSYDKYGADRYEIVTMTNSFHGRTMATLTATGQEKVQKGFAP
                     LMPGFSHVTFNDLSEVERAITSKTAAVLLEPIQAEGGVHVADRGYMQGLRDLCRERDV
                     LLIFDEVQTGMGRTGTLFCYEQFGMQPDIMTLAKGLGGGVPIGACLATETVAQAFSPG
                     THASTFGGNPLACAAALAVLRVLLEGKILDQGRRMGETLAKGLAVLKDRHRCVKDVRG
                     LGLLQGVELDIDGKAVVADCLARGLLINCTGDRVLRFVPPLIITEREIDRLLVALAQV
                     LSQRTTSSHH"
     gene            38484..39128
                     /locus_tag="PK224_06570"
     CDS             38484..39128
                     /locus_tag="PK224_06570"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HRB15436.1"
                     /translation="MKSCSQCRQENRDDARFCHQCGGAFAVESQETVVEADSAPSPQT
                     DAELWKAFIGPNADRYLETFKKFTGPSGPKFALTWHWPAFVFEPFLWFLYRKMYVYAL
                     IYAIGPAMAFYITQDLSADIIWRVIAGASANYIYFWHAKEQLAKIKGERATGGEARQQ
                     MLGELGGVQPYVVWVGVGLLVLKIGLVVAMFKDGPPDGSKNPPAKPHPASVTHV"
     gene            complement(39191..39658)
                     /locus_tag="PK224_06575"
     CDS             complement(39191..39658)
                     /locus_tag="PK224_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15437.1"
                     /translation="MAVWQFQIELVPKAVAEANSLRTGMVLTEEQRESVVWWRDQRLP
                     LTLLRDVEKMLPSGKTWSKDLEVLGSLETSCLTILREGESLVEVQFRFDLRETTIELL
                     ETAVQFSRDLQCWLISEGGKVIEPGLSALKDEIRESTAFQFVKDPLSFLSRLK"
     gene            complement(39725..40555)
                     /locus_tag="PK224_06580"
     CDS             complement(39725..40555)
                     /locus_tag="PK224_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15438.1"
                     /translation="MTERSPSDWTPLQEWWRAMGRRLQVEDRVEAFFRRALGVSQALA
                     GVAQAGGEVEYVLFVLVRYWIPVVLPPPGRERASKGDPDYWLESEQILKAAAVRLREL
                     KPLIELLTVANPLSTGGPDHSPARSPVVEVELANMLQGIAESAGSYGGPDYTSVIKTF
                     DPVPLRQTQPFKHNKKNSAELWVVFLLREHFRSVGLGKDRYWPLVAGLCAAAGITNPN
                     GQPYGPDELKSWWQKNWPRTYTQLEQTQAAPDLSGAAYQQDFDWFVSWIEWQRQRLSE
                     "
     gene            40738..41343
                     /locus_tag="PK224_06585"
     CDS             40738..41343
                     /locus_tag="PK224_06585"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRB15439.1"
                     /translation="MSKLVLIRHGESQWNLENRFTGWVDVPLSPKGIEEAKAAGKKLA
                     GFTFDRAFSSVLARANETLRLVLEGIGQTGIPIEKDKALNERMYGELQGLNKAETAKK
                     FGDEQVKIWRRSYDVRPPGGESLKDTAERVLPYYDSRIRPYVLQGETILIAAHGNSLR
                     ALVMQLEQLTREQVLELNIPTGAPLLYELDNNGKVLSHRYL"
     gene            complement(41372..41866)
                     /locus_tag="PK224_06590"
     CDS             complement(41372..41866)
                     /locus_tag="PK224_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15440.1"
                     /translation="MPKGAMTPAEVADALYKLIPRRVSVELLSEYGIEGQEEHEETMT
                     RELLSFTLYWVHAAVNAHIPRKYREVLFQRVLELIQADWAATFKLESVKWEDYLVEME
                     ERRALYAPVGDYEGGAMAASEEISDLLENQCLIQPEDRPKLLVLLPDLVPLDKYQELL
                     SQCV"
     gene            42022..42564
                     /locus_tag="PK224_06595"
     CDS             42022..42564
                     /locus_tag="PK224_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15441.1"
                     /translation="MRPVRALFIGLAFLGILWAAPTTAAEVQEPRWGFSTDLGLWSGT
                     TNDTTFALGFGLDYYMDQNFSFGGMALFTPVGDLTQIGIAGVAKYHLRLNSGFNLVPF
                     AGLGILHADLNRGSGPTKVDRNDTSHFIPLGMSLEYQVGPKIAFSTTLMVNLHHITLS
                     PPVPNDNTSVALLFGIRWGP"
     gene            complement(42922..43944)
                     /locus_tag="PK224_06600"
     CDS             complement(42922..43944)
                     /locus_tag="PK224_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRB15442.1"
                     /translation="MRRLRVLVLMHKDLVPPDQLNGQDLEGAAWKTEYDVVSTLRKLG
                     HDVQPLGVKSDLEVIRAAVEDWKPHIAFNLLEEFDGRAVYDQNVVSYLELMRIPYTGC
                     NPRGLMLARDKALAKQVMSYHRIPYPEFMVVPLHRMVRRPKYLPFPLIVKSISEEASL
                     GISQASIVDDDEKLRERVAFIHDNVGTGALIERYIEGRELYVGVMGNAHLQVFPVWEL
                     VMDKMPDEARRIATERVKWSRKYQDKYGICSCEARNLPDGVVDHIQHLAKRVYRALGL
                     SGYARIDMRMDKDGNVYVLEANPNPQIATGEDFADSAEKADLAYKDLLQELLHVGLRW
                     RPAQAA"
     gene            complement(43941..45002)
                     /locus_tag="PK224_06605"
     CDS             complement(43941..45002)
                     /locus_tag="PK224_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HRB15443.1"
                     /translation="MGRTKNHRGQGIGATDPVPVWAEWTDEQLLDLRMCDLDLRLEGT
                     FYQEPIAQLARELEARRLTFRPHFWISDEWFTPDGVPGIAVPFYLAHPRLAKLEASQM
                     LEVEGGTRDWCMRILRHEAGHAIENAYLLRRRRRRQKLFGRSSQPYPEYYTPRPYSRS
                     FVRHLDVWYAQSHPDEDFAETFAVWLDPHSLWKERYRGWPVMKKLDFMDRLMGELADT
                     TPVVTGRQLLDPLPRIYKTLRDHYEEKRKHYGIGRAPSYDTDLKKLFSDGPAYAKNPS
                     AAEFLRRTRKEIRRRVAEWTGEYQYTIDQVLEGMIERCQASKLHLTQHKEQAKLDFAI
                     LLTVQTMNYLHSGRHKVAL"
     gene            complement(45185..45652)
                     /locus_tag="PK224_06610"
     CDS             complement(45185..45652)
                     /locus_tag="PK224_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HRB15444.1"
                     /translation="MTSILFVCSGNIFRSLVAEYALKAQLGSRVGYLIGSAGIEALPQ
                     EIHPVVLAGLREKGVDPSRHRQRKLTRDLIEETAVVIAMGRNHQAWIRGEFGREVPLF
                     NHVCYGIDQPILDVHEIIPDWREQPDQSRDYLQFVIDHIWGGVPHLLNREPAL"
     gene            45731..46606
                     /gene="ttcA"
                     /locus_tag="PK224_06615"
     CDS             45731..46606
                     /gene="ttcA"
                     /locus_tag="PK224_06615"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HRB15445.1"
                     /translation="MQSTTTPPSPGTAQPTRHSNEIERVHTRLCRLVGQAIADYRMIE
                     EGDKVMVCLSGGKDSYGLLEILLSIRSRAPIHFDIIAVNLDQKQPGFPAHVLPEYLTR
                     RGVPFHIETRDTYSVVKRLIPEGQTTCSLCSRLRRGHLYRLATELGATKIALGHHRND
                     ILETLLLNLLFTGKMKTMPPKLRSKSGRHVVIRPLAYVKEADLARYAALLQFPIIPCD
                     LCGSQEDLKRKQVKTLLQDWDQRFPGSNDSMFAALGNIAPSLLLDRTLFDFSSLKADA
                     SPREPAASDSEDDLL"
     gene            47473..47910
                     /locus_tag="PK224_06620"
     CDS             47473..47910
                     /locus_tag="PK224_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15446.1"
                     /translation="MKRSTPSRRPAAEPPLWSIAAAAGVIILTPMLLYSLAPEGPIRE
                     GDTVFSSGAHKVSLFEPDRYRQAGYEATCMLDPKDPLIVTHSPSEDESGEVIIAQVQG
                     KSTIEWPFCPPQAELLVKRHHITQQPSILQDIRDGLLRLFKPS"
     gene            complement(47935..48480)
                     /gene="msrA"
                     /locus_tag="PK224_06625"
     CDS             complement(47935..48480)
                     /gene="msrA"
                     /locus_tag="PK224_06625"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRB15447.1"
                     /translation="MGQETTDIATLAGGCFWCLEAVYDQVNGVRSVESGYIGGQVDAP
                     TYEQVCGGHTGHAEAVRITFDPRLVTYRDLLNVFFVIHDPTTRNRQGNDIGTQYRSGI
                     FYHSPEQQQIAQEVIAAVTRERVYPNPIVTEVVPATRWYEAEAYHQEYFARNPFEGYC
                     AYVVGPKVAKFRQHYASLLKA"
     gene            complement(48513..50024)
                     /gene="zwf"
                     /locus_tag="PK224_06630"
     CDS             complement(48513..50024)
                     /gene="zwf"
                     /locus_tag="PK224_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRB15448.1"
                     /translation="MDLPQLRTPCVMVIFGIDGDLAKRKLIPAVYNLMAGHFLPEGFA
                     IVGLDRPEMTTAEFREKLDRVMGDYLPATVDRSVWQALSERVHYLAGDFQEGATYRRL
                     NELLQQVDASSGTQGNYIFYMATIPGLFGQIVTHLGEYGLTVEREGTWRRVVIEKPFG
                     HDLESARALNHELQRVLQERQIYRIDHYLGKETVQNILVFRFANGIFEPVWNRQYIDH
                     VQITVAESLGVEHRGRYYEQAGALRDMVPNHLLQLLCFLAMEPPNSFAADAVRDEKAK
                     VLRGVVPLSSLDVLRFVSFGQYGPGTSDGKAVTGYRSEPHVGSESMTETYVAMKLFID
                     NWRWAGVPFYLRTGKRMTRRLTEIVVQFKRAPFMLFRKTQVDRLVPNVLVIRIQPDEG
                     ISLRFDAKVPGPTVRIGTVDMDFQYADYFGNAPQTGYETLLHDAMAGDATLFQRADNI
                     ELGWAVVQTILDVWKSLPGPASFPNYPSGSWGPPEAEALLKREGREWWNGGQA"
     gene            50312..51139
                     /locus_tag="PK224_06635"
     CDS             50312..51139
                     /locus_tag="PK224_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB15449.1"
                     /translation="MMAGAGFVSGTTPQLTPRDVHIEVPADSLFGSLPVNRGLTVSIH
                     VPEPPVSRGEPLVAVFETPSSAPYTVPLEIDRSRHHYSATVDLGRLSGTMGTPPKATA
                     LQVLIGRRQGLHVEALVRRTVVVTLAIPGYADHRSARTDFANQMANGSGPPRNQPADL
                     ALLDGRVEEEELVGNGGLPQQEGYWKMLQGLIHKGMPDGAATRRGREIGRMPGIGFRL
                     YANGEAQLIEVERSSGDLELDQAAVLAVVNAHPFPPFPPGTSDTHVDVHVEIPGLPR"
     gene            complement(51173..51493)
                     /locus_tag="PK224_06640"
     CDS             complement(51173..51493)
                     /locus_tag="PK224_06640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB15450.1"
                     /translation="MERRDSPRYEIEVPLTFSGNAIAGSGLVTSLSNEGCHVVSEESL
                     PARACLSLHVEFPAPYEPMTVEVAEVRWTNATGFGLVFVHVHDEEQTRLQRFIDWLKR
                     THNN"
     gene            51872..51994
                     /locus_tag="PK224_06645"
     CDS             51872..51994
                     /locus_tag="PK224_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15451.1"
                     /translation="MEHLSEIVITVVAFAALIGQWMTDLHTMPNRNRPTSPEEQ"
     gene            52349..52711
                     /locus_tag="PK224_06650"
     CDS             52349..52711
                     /locus_tag="PK224_06650"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB15452.1"
                     /translation="MDMDLRSSTRVAVTYPVRLSGDSMIGQGTLINLSGPGCAVETTL
                     PVQPGDYLELHVMAPDQARPLTVGLAKVRWATEKKAGIEFIRVRRDEQSRLQRLIGQV
                     LEESTMEANTNGRELTAA"
     gene            complement(52783..54174)
                     /locus_tag="PK224_06655"
     CDS             complement(52783..54174)
                     /locus_tag="PK224_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HRB15453.1"
                     /translation="MTDHVEPSGTDSRPPGFLNRIHQSNKQYLPQWLGVPAHIHHVAY
                     RMANPPVERPNSRREFQQLLQALEISEGAIEDRFGYGFKVAANGDRLVVVWEAHTEYY
                     SYQVWHVVRDATTPLDFGPITFPGYMMPLSPLGIRVNALDLLFLPQELPLEQELPARL
                     PGAMVYGSRILGDDIVAVTSFTPDEFDRERYLICSTSEQALRQQVAKIVDTIVAIENY
                     YHLVMLPMKAFSRAVDQIHDYEQRHLYQRAVLIEQLGGTTPPTMQKWLTVLTQDLLQV
                     SRLAESMRYRLSASVPYDRIVQSNLAALQERPYPPLRQISEYVSWKITGVTEGYQQLL
                     RRIDAMEKDFEATVAVLRTHIELRLQEQNIQLQDQNMKLLISVDSTTRAQAILQRTVE
                     SLSVIVITYYLTGLGSYVLKACYEMGWLKNANVATAIFVPIAFATSFTLMTVGRKIIV
                     KRMADSAKDTAGH"
     gene            complement(54299..54463)
                     /locus_tag="PK224_06660"
     CDS             complement(54299..54463)
                     /locus_tag="PK224_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15454.1"
                     /translation="MNQRFGITVKFLISADSPEEAEEIIESACEKMATSIHEGDVSYE
                     GIEDIEEEDE"
     gene            complement(54966..55703)
                     /locus_tag="PK224_06665"
     CDS             complement(54966..55703)
                     /locus_tag="PK224_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB15455.1"
                     /translation="MKYRGASQLMLGGMAMVLLTAPGCSNMKWFQSGSDEASNSGSSL
                     SASSGASADASGGYGRGESGRDGQYPSLGREQDMTAAEGGRLRGFSPLVNGQIAGEER
                     LSRSPIGSMLTSNERDEKWAAEIRREEAAAMEAGLKDVFYGYDRYSVSDNDGVVSLTT
                     NADWLKENPKALLKISGHCDERGTHDYNLVLGEKRAKAAKSFLVDLGVNAKQVAIVSY
                     GKDRPFCADHDEACHQQNRRGHMLLRK"
     gene            complement(56046..56489)
                     /locus_tag="PK224_06670"
     CDS             complement(56046..56489)
                     /locus_tag="PK224_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15456.1"
                     /translation="MGYRSIQVRIKEHAGQKKGGATSSDAPRRRLGRQSTPKLSEALK
                     MGAFTLPDVDEAWSKPDHNKLPTGCAITRACVAAGYTGSNELYEVAKFINTTWPWTWD
                     YASDRTSFCDAGIAIITDVTALSGEGRTMSEIGNWMAEIESNRLS"
     gene            complement(57187..57456)
                     /locus_tag="PK224_06675"
     CDS             complement(57187..57456)
                     /locus_tag="PK224_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HRB15457.1"
                     /translation="MLIIKPTSRFLKDLKLAKKRGQDIDELEDIIDLLQFQKVLPVRN
                     RDHALSGNWKHHRECHIAPDWLLIYRTDDEFLYLERTGSHADLFE"
     gene            complement(57459..57737)
                     /locus_tag="PK224_06680"
     CDS             complement(57459..57737)
                     /locus_tag="PK224_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HRB15458.1"
                     /translation="MRKTSHLSMSDRINARIDMGLKKRAVKIFDRLGISEAEAIRLFY
                     AQVELHQGIPFPLTIPNATTIAAFDETNKPEKLPSFKTFRALRNQTGV"
     gene            complement(58032..58763)
                     /locus_tag="PK224_06685"
     CDS             complement(58032..58763)
                     /locus_tag="PK224_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRB15459.1"
                     /translation="MKIVLDIETLQASEGEWAYLQGIASAEEAMHIDPTLIATQFAKE
                     YEKSRFDGTFSRIICMGILVVEDSFSPVEAIAWCGNDETAILRNFWSKMARINPVQVI
                     VHNGLGFDLPFILKRSIIRNVRPTVDIALTRFRTTPVFDTMAVWANWDPRAFIKLDVL
                     ARALAVESKSGSGDQVAKMWDEGLYADIARYCLQDTHVTYCCYCRMNFREISGCISVL
                     NNSSIHIVGESVNATSARTLVGTIR"
     gene            complement(58741..59691)
                     /locus_tag="PK224_06690"
     CDS             complement(58741..59691)
                     /locus_tag="PK224_06690"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HRB15460.1"
                     /translation="MKWAEIKSKMRSVEMLAFYLEVSQGTLFRLSRTASHFYRQKDEP
                     KSDGGVRTYLIPSGELKRVQSLIHKKVLLFAPFHKAIHGYRRKRGQVTAALPHVEKPM
                     LLTADIEKFFPNIRPSMVYDAFRQVGISDSVSRLLVDLCTHNNQLPQGAPTSPLISNL
                     IWSRPARRIQGFADQQHFDLTILGDDAFVSGAPRAKKFKNLVRRIIQEEGFSVNERKT
                     RALPSTGRQVTAGLVVNKKPNVKKEYKRALRKMLHSCASNECSDVMSSEIKVNKASLA
                     GKIAFVKHINPAQGQRFQAEFERINSRDSKKANSEDRPGH"
     gene            complement(59694..60026)
                     /locus_tag="PK224_06695"
     CDS             complement(59694..60026)
                     /locus_tag="PK224_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB15461.1"
                     /translation="MNKHEDLGIGPIIKILRAAIGLRQKDLADKVGIQPHYLSLVEAG
                     KREPSLAVLRKIANELDVPVSLLFWEAGQDPEGVSSEKEGRWKSLRSLLVEMERLRLQ
                     GTREERAI"
     gene            complement(61177..61419)
                     /locus_tag="PK224_06700"
     CDS             complement(61177..61419)
                     /locus_tag="PK224_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15462.1"
                     /translation="MIWHRCDLKLPSLFGPACCGREATTYDRARKAWLCPEHTNERLC
                     EFCQFERAWVRLCYPEKDVTCEIWLCVECAGLTGRR"
     gene            complement(61422..62135)
                     /locus_tag="PK224_06705"
     CDS             complement(61422..62135)
                     /locus_tag="PK224_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15463.1"
                     /translation="MSRWKEFRREPVAGEWTRKQKRGYHRVRSLLWFWECHQFQVLWV
                     TLSTAEGGDAEKLTYHHKQLRQRIERQLGFQGLEYYQVRTEEGHGVLHIFWAWRVPDG
                     ERARRFWISQEWLSSQWQALHGAPVVWIKAYQPSHRSRNRLSRYVISQYVQDQCGYVN
                     MCWSWKRSLGFPISRLWEEMRHQWSTRNAYRRIRGEIEIPRIVFIKTWEDLLSGHPIW
                     FSGTILQLVLGKGLVYQEV"
     gene            complement(62132..62341)
                     /locus_tag="PK224_06710"
     CDS             complement(62132..62341)
                     /locus_tag="PK224_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB15464.1"
                     /translation="MVPSTKLITIREAAERLGLKESTIRKYILKRQIAYVKPSVRAVR
                     IPIEELERILAAGLRPAIPQAEGVR"
     gene            62545..63210
                     /locus_tag="PK224_06715"
     CDS             62545..63210
                     /locus_tag="PK224_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15465.1"
                     /translation="MSAQGKAAEEFQQEYQKAIERIRTMSDGAVGWVLKFLQMDLEAL
                     TPTEWTLVAFEVAAFVDETGDRYGGMVAPESGWSVEGVPHAKNYQTIPSRKEAQDMQA
                     TVLEHLELYWHEGYTAFTFPQMTLVVVSPGSFSDETGTIFVSAKRKAKEFEYRFVHLL
                     AQSGDYIRRCPECDKIYLAIRRDQVYCHPRCQNRVAARKWREAQKTGERKESRRGKKS
                     GKG"
     gene            complement(64311..64387)
                     /locus_tag="PK224_06720"
     tRNA            complement(64311..64387)
                     /locus_tag="PK224_06720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64351..64353),aa:Arg,seq:tcg)
     gene            complement(64475..64993)
                     /locus_tag="PK224_06725"
     CDS             complement(64475..64993)
                     /locus_tag="PK224_06725"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRB15466.1"
                     /translation="MNLVLIGYRGTGKSTVGKVLARKLGRTVVSTDAEIVKRAQLSVP
                     EIVKQFGWDHFRDLESAVCRDFAARDHLIIDTGGGAILRPENVDALRRTGTLVWLTAT
                     VETITRRIGGDTQRPSLTGTKSFTEEIRDVLNERTPKYQAAANHVVSTDGASTTEVAE
                     RVLQLISRGSAV"
     gene            complement(65060..65914)
                     /locus_tag="PK224_06730"
     CDS             complement(65060..65914)
                     /locus_tag="PK224_06730"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRB15467.1"
                     /translation="MDITTQTQWCGIIGNPVEHSLSPAIHNAAFQKMGLDLVYLAFRV
                     EALGDALKGVRALGNARGFSVTIPHKVAAIPFLDEVEPTAKHIGAINTIVVEEGKLKG
                     YNTDASGALRALKEGGALLDGHRVLILGSGGAARAIAFALATGTGIAGLTILGIEEVE
                     RQKLVKDLREKTNIPIEDGPLTLDMLRNWVPHVRTLIHCTPVGMSPRVEESCVPTNLF
                     RPELTVMDIVYNPRETKLLQEAKAAGCRTISGLEMFLNQAVLQFELWTKQPAPADVMR
                     RVLESRFG"
     gene            complement(65928..66263)
                     /locus_tag="PK224_06735"
     CDS             complement(65928..66263)
                     /locus_tag="PK224_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15468.1"
                     /translation="MSGMSRMSAVVVPALFLAAVLSGLLESSVEPVRVEGGTEFVGTI
                     VSVDTTSGKFAVKKDGGGSRFTFVANEKTHFDGGAKAVKDLKKDDHVAVLYQVQGSQY
                     VALNVALKK"
     gene            complement(66354..67115)
                     /locus_tag="PK224_06740"
     CDS             complement(66354..67115)
                     /locus_tag="PK224_06740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB15469.1"
                     /translation="MNRLQGKVAIITGGNAGIGEAIAKLFADEGASVVVTGRRKEELD
                     RVVKGIGVNGGRALAVVGSVTDEAHVQDVVAQTLRTFGKLHILVNNAGIGDFGKRLHE
                     MDDATWAKVLDINLTGVFRMTRAVLPELLKGGGGSIINISTVASLVGIRGLSAYAASK
                     GGLDALTRSIAVDYAQDNIRCNVMNPGLVDTPMAAPLMADPASLEPIMAQYAIRRPGK
                     PEEVAKMALYLASDEATWVTGATFPIDGGMTISKG"
     gene            complement(67112..67990)
                     /locus_tag="PK224_06745"
     CDS             complement(67112..67990)
                     /locus_tag="PK224_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HRB15470.1"
                     /translation="MSQLQNGERVHLVDKKGRQYALTLKAGDIYHFSGETIPHDELIG
                     KPDGTVVTLSKGKRFLALRPTFGEYVLKMPRGAQVLYPKDLSLIPMWADVYPGARVFE
                     AGTGSGALTMALLRAVGPTGLVVTYEARDDFARTALTNIERYMGPVSTLMPCRKNAYE
                     GIDVLEDGRLFDRLVLDLPEPWQVVPHAAKVLRSGGIYLSFVPTIPQVMQTVDALERT
                     SVFGMIETFETLLRTWSIQGRSVRPDHRMVAHSGFLTVARKVEEGWWSRPSKTVEAEA
                     AGEQDDEAHEGEDAEA"
     gene            68289..69548
                     /locus_tag="PK224_06750"
     CDS             68289..69548
                     /locus_tag="PK224_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB15471.1"
                     /translation="MKDALAIQDDTDQTVAAEVDPDADDPEASTLLDSIVQGNQAETE
                     ADSEDSAPEKPARSASSPFLLESLYFRSFGERALLERDEEIALAKQLDLGTRQIRQTL
                     QQAVKAGGRLKRTDRTAECLETLKTVRRLSGLSATVLNQAERALEELQLDATTGGRVP
                     VSVRRELDAALTQLKASRITLETAKDELVRCNLRLVVNVAKHYTGRGLTLLDLVQEGN
                     IGLMKAAERYQHRKGFKFSTYATWWIRQGITRSLADQSRTIRIPVHQTEASNRILRAS
                     RRLVQQLGRQPRLEEVASTMRMRPDRLHETIQAFQEPVALEHRVGDGGTELGELLPDQ
                     QAVPPDAHVNRTELTREMDRILGTLTPREQTVIRLRFGIGEDQARTLEQVGQHLSVTR
                     ERIRQIEAKALKKLKTPMVKEMFAAIK"
     gene            69676..70749
                     /locus_tag="PK224_06755"
     CDS             69676..70749
                     /locus_tag="PK224_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRB15472.1"
                     /translation="MLTALYSRNGARPDRTRWFAWGLFSLSILSLCTPANGAGHNPNE
                     STSLTELSVPAVVAKVRPSVVTVLTRGIPQGGSQHGAPPGSGSGVILDAGGHILTNNH
                     LVQGVTSVVVGLSTGRLTPGRVVARDFLLDLALVRITAPDLVPAEFSWRTSFEIGETV
                     IAIGNPLALKGGSTVTVGVISALDRSVLTPEGETLYDLLQTDAAINPGNSGGPLVDRY
                     GQVVGINVAIAPSAQAISYAIAMEAVTPHLQSMMDRGSVLRPDLGLIPLTITPSVAAS
                     FDLESDRGILALSVDPAKPAGRAGLQSGDVVTAIDNHPLYNIGDFWHAVSRASDQLSF
                     QITTQGKAGTGTITVSRPGPARP"
     gene            70753..71580
                     /gene="lipB"
                     /locus_tag="PK224_06760"
     CDS             70753..71580
                     /gene="lipB"
                     /locus_tag="PK224_06760"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRB15473.1"
                     /translation="MKLDQPSQTESAHPVSKVPAQAQPPRAGILIRCEPLPYEIAWSL
                     QRACRTERAADRCEDLLLLLEHPPVFTVGRTTQDQHWPGESCLTQESGIPVIRTERGG
                     SITYHGPGQLIGYPVLRLSDFCAGPKAYVHRLEDVIISTLAEWGIAGHRRERLPGVWV
                     GGDIPSKIASIGVRISQGVTTHGFALNVCLDLAPFSYIVPCGIADCRVTSMAALLGEA
                     PEMGTVQERLAANFAERFHLAWAERIGPDRFLALSDASPTIAQGPLPQRATHQQGVT"
     gene            71580..72836
                     /locus_tag="PK224_06765"
     CDS             71580..72836
                     /locus_tag="PK224_06765"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRB15474.1"
                     /translation="MNEFDTHTFRLAVAQFNEAAESMRLDTNLRERLKLPQRSLLVSI
                     PVKMDDGHVEVFTGYRVQHDSARGPCKGGIRYHPDVNLGEVAALAMWMTWKCAVADLP
                     YGGAKGGVKVDPKKLSRGELQRLTRRYAAEIFPLIGPDKDVPAPDVGTDQQVMAWIMD
                     TYSQQVGYAVQGVVTGKPLSIGGSLGREEATGRGVSYVTLEALQHLKLDVSKATVAVQ
                     GFGNVGSNTALIMQQAGARVVAVSDVGGGLYNPKGLDIADVLHRYRDKHEPLREIKLG
                     ESISNEELLQLDCTVLVPAALSEQITHANASKLRCRILAEGANGPTTLEADRILTDKG
                     VFIIPDILANSGGVIVSYFEWVQDVQRFFWKAKDIQDRLQDIITSAFHRTLQFSVEKR
                     TTMRMAALMSGIDKVAQAHLQRGLYP"
     gene            72868..73254
                     /locus_tag="PK224_06770"
     CDS             72868..73254
                     /locus_tag="PK224_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15475.1"
                     /translation="MTRYILAAIAGIWMADGLALLTVPLLVIKRVQESLLHSPQLLRW
                     EAVGIGLGAILILWSGPIPYQPLWWITGGAMIIKGCFLTWGPAAWRTPLLDWCFAREA
                     IDYRFFGLWLCMLAVLLLHALGLLHR"
     gene            73291..73584
                     /locus_tag="PK224_06775"
     CDS             73291..73584
                     /locus_tag="PK224_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15476.1"
                     /translation="MSHQTIASLWEEHSREGWPRFSSPNEGQLMTLDTVIGGCAVFYL
                     DGQTEMDSQRIAILQDCVADLDTLLDDLTEDSLGYFQRLRRLAAALVQLNQAT"
     gene            complement(73645..74103)
                     /locus_tag="PK224_06780"
     CDS             complement(73645..74103)
                     /locus_tag="PK224_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HRB15477.1"
                     /translation="MGAIVLSALAVLAGCADTGSLNGAGRPAAGATATKSLYDRLGGK
                     PAITAVVDQFVANVAADGRINSRFATTDIPKLKGHLVDQVCGATGGPCSYRGRDMKST
                     HLGMQISSADFGALVQDLVAALDKFNVPAGEKSELLGILGPMKKDIVEVP"
     gene            74305..75252
                     /locus_tag="PK224_06785"
     CDS             74305..75252
                     /locus_tag="PK224_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRB15478.1"
                     /translation="MELRQIEYFMAVAAHQNFSRAAEHVHVSQPSLSIQIGGLEKELG
                     TRLFDRLGRKVVLTQAGELFHVHAERALRELDQAEQVVQELLGAKRGRLVVGALSTVN
                     SYLIAPLVSRFKQRFPDIHLQVHAQASADVVSGLLSNRLDIGICLLPLTHPQLTTVPL
                     FEERLALVAPAGMNIGKRRARIQDLARLPLVLMPVDYCLRKMVEAECAKAGVHPQVVL
                     EMSSPEGILQAVAEGTGATILPELYVRSRLPGMDLQVIDLYDPTPRHAVGLAYLTKRH
                     RGLAAEEFAAICHTTMRDLQSDPEQSRRNRPTRRAAGQK"
     gene            75429..75959
                     /locus_tag="PK224_06790"
     CDS             75429..75959
                     /locus_tag="PK224_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15479.1"
                     /translation="MMQRKWAFTALVAMMGSLYLAAMVSAGPYELSKDDLTDPKGIAS
                     PDVSLFGIKLGDDEGKALEGLVNEKIPGIKAEQEATFIFLLDQRKPTGPMAGVRVQDG
                     KVDLIFINNRFAYKTRGIFRNVLNSESPDDVRKLLGKEDYGDENVMGAILAYDKQGFQ
                     VNYLGKDVNVEFSLPH"
BASE COUNT        16610 a        23664 c        20576 g        15118 t
ORIGIN      
        1 tggaccgcct ggaggataag gagctgattc gacgcacgcc ggtcgccgat gaccgacgaa
       61 gcacgcgcat ctgcctgacc gagaaaggcg accgcctgtt caagaaaaca tttgcggccc
      121 acatcgcatt tttacgcccc tttttcgaac gagcgctcac caccgcagaa gcggagcaat
      181 tgcgcacgct cctccgccgc ctccaccgga gctttcagga gaagccgacg gcatgatccg
      241 ggtcacggtg ctgggctcag gcacaaacgt tcacccgaca cgcgctgcgg ccgggtacct
      301 ggttcagacc gaccagactt ttctcctcga cttcggaccc cgcacgctca gcaacttgat
      361 caccagcggt gtcgatcggc accggattac acatattctg ttttctcatt tccacgccga
      421 ccactttgcg gatttcattc ccttcttctt cgatgccgtc atcttctgca agtaccaagg
      481 cggcaccaga cctccgttga ccctgatcgg tccgcatggc accgccaagt tgatgcgcgc
      541 gatgatgacc acattcccga gcttcaaccg cgctccgttt cgcgtcacga ttcgagaggt
      601 ctccgatcgc agcttccgcc tcggcgagac ccgaatcaaa cccgggacgg tgacccatgc
      661 gcctcgactg cattgcgtag gctaccgcat cgaataccag gaccatgtcg tcacctactc
      721 gggagattcc ctctattgcg acaacctggt ccgtctctgc cacgaggcag atcttgccat
      781 tctggactgt tcctttccgg aaaaccgccc gggcgcagga catctccacg ccgggctctg
      841 cggccgcgtg gcgcaggaag ccggcatcaa ccaacttgta ctgtcccatt tttatccgat
      901 tgcagaacgg tatgacgtct gcgcacaggc gggaaactat ttctcaggac gcattcggat
      961 ggcgcgggac ctgctcaagc tcaaagctta gctaggaacc gtcggtggtt cgttctagaa
     1021 cataaaagag agaggcacgc tggcgagaac agacgagcag gatgctcaaa aagcccgtcc
     1081 agcaaggccg cagcgagtga agatccgagg cgtacccttg cggtacgttg aggatctgaa
     1141 cgaagcgaga acgaagctga cggacttttt cagcatcctg ctacagccct tcttctcgtt
     1201 gctcacaccc caatatttca atgaagcgag gcaggcgggt ctttttcagc ggctcatcga
     1261 gcttattgta gatggcgagc agattcttga tcatgcgggc gagaatcgcg cgagtgggac
     1321 tcttctggag aaaccggtct tcgaatccgt aaccggcttc cgtcaggaaa cgggcgcagt
     1381 ttttttccgt cagcagtgcg cccccgttga aacaatcgat gaagatctta taccgatcgg
     1441 agtcgaactt gacgaggaag tggcccggca tgccgatccc ctgaaccggc aggtgcaacc
     1501 gctttccaat gagcagatac acggttgaaa gactgatcgg gattcctgtt cgccggtcca
     1561 tcacacagtt gagataactg ttctcgacct catagtagtt cttggtgttt cctctaaacc
     1621 ccgcttcggt aaacaggtag cgattgagcg ccttgacagt ctcttccccc gagacccggg
     1681 acccgatctg ctcccgcaca tcctgagcca tacgatctaa ttgtcgctga tagctctgga
     1741 cgtcaagcgt cggataggca taccgcgcca acaggaacgc ccccgtttca agatcgatgt
     1801 gatcttccgg aagggcgatg agatcccgga gttcttcctc cagcctggtc ccgcggattt
     1861 cctccagcac cgtgacgata cgatcggcca tctccggctg ctcgatctcc gcttcctgca
     1921 ggagcggcac ggcggagtcg ccgaaatcga tgagcttccc gctgatcgtc ttgacgatcc
     1981 gttcgtcctc atcgccgagg agtcgaatca aggcgcggat ttggctttca ttcacaaaca
     2041 tggaaggact ccgctgcgca atcccattca cccgatcgcc cgccggaaag cgcgcgcgcc
     2101 gccatagagg cacagcgcat cgaacaacac catcaccccg atgaccatgc cgacatgggg
     2161 cagggcttca gcccatccct gcagaatgag cggccgcacg gcatcaatcg cccaggtcat
     2221 gggattaaac tgcgcgagaa aacccatcca gcccggcatc gccgccagcg gcaccagggc
     2281 cgagctcaag aaaatcatgg gcagggagag aaacccgagg accgagaaaa aatcgccgtg
     2341 gcttttcact gaaaaggcca tggccatgga aatcgccgtc agcccgacac cgaacatcat
     2401 gccgatgaac aagatcgtcg ccacccccaa caatcccgtg accggctgca ccccgaacaa
     2461 ccaggccaca ccaagaatga ccagcacttg caacgacgtg atggtcgtca caaaaatgaa
     2521 ccggctcaga atcaccgacg tgcggtgaat cggggtggac atcagccgtt cgagaaaccc
     2581 gttctccttg tcgaagagca gatccacgcc accggccaat ccgttgttca acaccgtcat
     2641 aacgaccaca ccggcggtca gaaagctgat gtagctcgac gcttgcgtca cctgcatgtc
     2701 tgcggcgcgt tgaaacaaat tcccgaagaa gatcaaccag aataacatcg gctggaccag
     2761 ggtaaagagc atgctgaact tttcccggct cagccggcgc acccagcgca tcgtcagtgc
     2821 ccgaatctcc tgccaatatt ctttcataga cccttcacaa cgctcttgat cgagccttac
     2881 tgaccctctg ccttcggttg gtcgtctttg atagaccgcc ccgtatgggc aataaacaca
     2941 tcatccaatc gcggccggtg atagtcgatg aactccagct gacaggtcag acgattcgcc
     3001 gcttcaagaa tggccggcag ggccttctcg ggagaatcga ctcgaatatc cagcccggtc
     3061 ggggtggtcg tcacagctcg aatggccggc agatttttca ccgccgccgc aagcaactca
     3121 acacgatcat gctcccgcac cgtcagcgac accaagtctc cgcccaaacc ggctttcaac
     3181 tcgttgggaa cgcccagggc cttgatccga cctccgtcaa tgatagcgat ccgatcacag
     3241 agctgatcgg cctcatccag atagttggtg gtcatgacga cggtaatgcc ccgctcccgc
     3301 atggcccgga catgatccca aatgcgcaac cggctttgta catccaagcc gagcgtcggc
     3361 tcgtcgagaa agaggatctt cggatcgggc aagagaccgc aggcgatatc caatttccgt
     3421 ttcatgccac cggaataggt cttggcggga cgatcggcct gctgctctaa ttcaaccagc
     3481 ttcaataatt gggggatacg tgtggacgcg tcttcaggcg acaggtgata gagatcggcg
     3541 agtagctcga gatgttcccg ccccgtcagg aaccgatcga tcgcccgctc ctgaggcacg
     3601 tacccgatcg tcttcctgac gcggtcggca tccttcaccg aatccatgcc gagaatcgtc
     3661 gccgacccgg atgtgggatg gagcagcgtg atcaggatac ggagtgtggt actcttaccg
     3721 gcgccgttcg ggccgagcag accgaatatc tctcccgcat agacctgaaa ggacaggtca
     3781 tcaacggcac ggaccttctc gtacgttttc ccgagatgcg acacctcgat ggcgaccgcc
     3841 cgcgacatca gccccaccct ccggcaccgt gccgctccgc cagcttgaac tggatgaggt
     3901 cgctgagacg gcgggcctgc tgcggcacat ggtccgcttc cagcaaaaac tgcttctcca
     3961 gcggggtgca gtcgagatac gtcgagaggg aattcacgaa cacctcgtcg ctcacatcgg
     4021 gccggaccag gctgtgcagc ggcgaggctt cctcatcggc cttgagatat tcgtcgagca
     4081 cgtccgtcag atagcggcgc agcgccggct ccatggagac agcaccttcc cgcggcttca
     4141 gcgcgacccg ggcttcgcga taactcttct cataaaactc ttcgtgtatt tcatagcgct
     4201 caaggccttg gaggaggatg ttcgagcggc catcgggcaa ggcttgaaca ctggccaacc
     4261 gtccgacaca cccgatcgaa aagatcgggg gatttccgtc gtactgttct tcccatcctt
     4321 ctttaagcaa cgccatcccg atgcattgcc ctcccgccgc cgcatccgct accatctggc
     4381 ggtagcgcgg ttcgaacaca tggagcggca gataggtttt gggaaagaag acgaggttgg
     4441 gaaggggaaa cagcggaatg cgctccggca ccggaaacgg ctgcgccttt cccgataact
     4501 cccggctcga ctggtcacgt tcgggctcaa gaaacatggc tcacgatagc tgatgctttg
     4561 caaaattgtc aacgctgccg ggcaaggcag tcacggaaat gaccctcgca aaaccctcat
     4621 gaatactggc gttccgtcgg tccctcatca aaatatggca ccgcatcata ggccgccact
     4681 cctcgccgcg cacaggcgac agccggtgag tcggacgtga tcacagtgcg ccattctgca
     4741 ttcgccgccc tggcggttat ggtcgtgctt ctgaccggca ccgttcattc tgtggcaacg
     4801 gacaaccagg caccgtcatt tgacaccgcc aagcccggct accggtatga atttcctaaa
     4861 gaccatggcg cccacgaacg gtttcgtact gaatggtggt attacaccgg ccatctcacg
     4921 gctgccggag gccggcagtt cggatttgag ctcacctttt tcaggcgagg cattccaccg
     4981 gaacaagtcc ggactcaacc ctcgcagtgg tcgattcaac aactctatct ggcccacttc
     5041 gccctcaccg acctcgacgg caaccgattc ctttttgccg acaaactcag tcgcgaaggt
     5101 ctcggaaagg ccggcgcgga gacggaccga ctgcatgtct ggctcgatcg ctggtcagcc
     5161 accatggatg atcccacgtc gtcccgacag aagctccagg ccgccaccga tgcatttgcg
     5221 atcgatttgc aggtcacccc ggggaagccg cccgtgctcc acggccggga gggcatcagc
     5281 cgcaagggcg cgcgacctga gcaggcctct cactattatt cgttcaccca cctgacaacc
     5341 gagggggaga ttcgcatcgg cgcggacacc ttttccgtct ccggtctcag ctggatggat
     5401 catgaattcg gctcagccga cttgggacgg gatgtggtgg gatgggactg gttcagcctg
     5461 cagctgagcg actcgaccga actgatgtgg tattccctgc ggcgggccga tggatcggcg
     5521 gaccccgtct ccagcggcac cttggtcttt gccgatggac gaactcagcc gctcacggcc
     5581 caggacctca ccgttgagtc gctcgactat tggacaagcc cgcgatctaa ggcgcagtat
     5641 ccgcaacagt ggcgaatcac ggcgcccagc ctgggactca atctggatgt acgatcgctg
     5701 ctcgccgacc aggaattgaa taccgcacgc agcacccgcg tgatgtactg ggaaggggcc
     5761 gtatccgcca ccggacaggt ccacggcaca gccgtcacgg gacgtggcta tgtcgaactc
     5821 acgggatacg ccgagcgctt cacccagcga ctgtagccac tcgcctgatg tctaccttga
     5881 ctcaatcgcc acgctatggt taggtctgcg gccattgtga tgacacgacg aactcgaatg
     5941 gctgccctgc tccggacatc cgtctgcctg gtcggcttgt tcctcctggc ctgcgggtcg
     6001 cgcgaagcgg cggtggtctc gatcgcgctc catccgtcga accccaacat cgtctatgtc
     6061 gcgacgaacg aagccgtgca caagacccgc gacggtggcg cgacctggga acagtttcag
     6121 gcgttcacgg cccgccgcgt gacgaccctg gcgatcgacc cgaaactccc tgccacggtc
     6181 tacgcgggca caatgggcga tgccatctac aagagcccgg acggcgggca gcattggctc
     6241 ccgcacaatg tcgggctcaa agagcatgtg tccttcgtca atgaaatcat cttccatccc
     6301 gacaataccg agctcatcta ccttgccacg acggtcggcg cctttgtcag caaaaacgga
     6361 ggccgggaat gggaagagcg gatggcgggc atgaaggaag tgcatatcgt cacctgcatc
     6421 accatggatc atcaacaccc tcgcgtcttg tacgccggga cgacaggcgg cacctatcgc
     6481 agtgaagacg gcggcacgac ctggcagaag aaaaacaacg gactcatacc tgacgatgtg
     6541 ctgaacgccg ccatggcgct cggggtgaat atgctggcgg tcgatccgcg taattccgac
     6601 atcgtctacg ccgccaccac caaggggctt tttcgatcca ccaaccgggc cggctactgg
     6661 gaacagatcg gccagaacct ggccaatcaa ttcatcagca ccctggccct gcatccgacc
     6721 gatcccaaga ttgtgtacat cggtggcccg gagggtattc tcaagaccgt cgatggcggg
     6781 aaaacctggc aagcccacaa ccagggactg acgaccatga atgtgcggac gatcagcctt
     6841 agcccgagcg atcctcagct gctctatctg gggaccaatg gtagcgggtt gtatcggtcg
     6901 accaacggcg gggaccagtg gacgccgatt cccctgacca gtcacgcgca agcggcgccc
     6961 gcgccgtagg attggcgaga gctcccgatg ccgatcgtcc tgctcatcag acatggagaa
     7021 acggagtgga atcgatccgg ccgggtgatg ggcgatcaac cgattcccct caaccggacc
     7081 ggcgaagaac aggcccgcac ctgcgccggc atcctgtctc gcaccccgat cgcggccatc
     7141 tacaccagcc cggtcctgcg ggccgcgcag accgccgaga ttctacgtgg gccacaggag
     7201 atcccgctac atcaggttcc cgggctgagt gagatcggcg tggggaattg gatcaatcgc
     7261 tattggcatg aattcgccga cgacccggcc aagcgggagt ggtacaccca cccggaccgg
     7321 gcccggcctt ccggcggaga aacgctgcgc gaggtgcaac agcgggcggt ggcggcagtg
     7381 gaacaagccc tcgcgcccgc ccacgacagc cccgtcgtga tcgtgtccca cggggatgtc
     7441 attcgggcga ttctcgcgca ctacctgcag ctcgacctgg cgatcatccg ccaggctcgc
     7501 atcgatcacc tggcggtcag cgggttggac ctgacaggca ccgcagccca gctgctgttc
     7561 ctcaaccatc gccccggctt agaaacgctt ctgtagtcga ccaccctacc ttctagggtt
     7621 gggcggcagc aggggccgca gcttctgcgc ccttgccgta gtaccggctg ccataccgtt
     7681 tcaattcctc cgacaatgcc gaccattcct cggctgtcag caaggacttc atcttgaacc
     7741 gaaggctgag catccgtgcg gacgaacgca tccggttgtt gttcaactcg tccagaattt
     7801 tcgtaaactc ctccggcgtc gccgcatagt tggcgttcag ctcgtagagc gcgcgatgag
     7861 actggcgatg ctgctcccgt gccgacttga tctccgtgat catctctccc atcaccgtat
     7921 tgacctgctt cgcggtctcc ggatttttga catgcttttc aaccaagccg ttcacatcct
     7981 gagaagcctt gctccaataa tagtcgccct tcccatgtcc ataactcccg tggtgatggg
     8041 atgaacaacc ggtcaatccg gtcacaagca cgccgatgag gacaaccaag gatcgataac
     8101 gcattacagc ctccatacat aagaaaaatg aatgcctcgc acgaaaacgc gttgcgccgc
     8161 acgtcgttcc tcccgtaagc ttcctttgaa cagggcaccg cacaggagac cgacatgggc
     8221 acacagaagg accaccggca gggaacaggt tcggtggaat cgctccggga cgaattccgg
     8281 gagcatctcc acatattcta cagcacgctc aagctggccg cgccctacga cacggtcgag
     8341 aaagccctgg gggccctgac cgcgagactc aaaacgttgt cgccagaaga actccaacag
     8401 atatccacgg actcgtcctt gcgatggacc tgcttccggc aggccttcgt cgaatccggc
     8461 ctgagcgcga aacatcgcgg gatcatcgcc ggcctggcac gatccggagc ggcactggac
     8521 ctgccgcctg aattcgagcc cctgctcaac ctctatcgtc cgaccagcta gcccgcatta
     8581 ttcatgagcg cttctgctgc aatcgcggat tcgcagctgc gacaggccta tcggcgggcg
     8641 ggcacgccgc tcgtctcgca tcgcgactcc gcaatctcgc gacgaaccgt catgaataat
     8701 gcgggctagg ccgccccttc actcttcaac gccacgtcac gcagctccgt atagacctgc
     8761 atcagtctcg tattttcgat gcgataggcc accgtaatcg tcaacaggcc gaaggcgacc
     8821 acgatcattc ccacaccggt gacgccgatg cccaacagta cgctccccag tgaatcgaac
     8881 ccgccttgga gcaggtaggt aatcacgaac ccgagcgtcc cgaaccccac aaggctgaac
     8941 cacagaaacg tgagaatcga agacgaccag ggcgcccgct tcgtgcacac catcttcagt
     9001 ccctccgatt ccgccgccca ttcgatggag cccttcaacg gccaggccgt gcggaaccgc
     9061 agagagaaaa gacgatgggt cggcctgatg atcatccggt ttgcctccgg aaagacccgc
     9121 gcgacgccgt ggggcagact cagaaaaccg ttctggtcga acgtgctcag cagttgagga
     9181 acggtcagcg cagcaaaatg atcctgcacc cgcccgatcc catacccgta ccggctggca
     9241 tcggacgaga gacgggcaaa gtacatccag tcaccgacga tcaacccgac aaacaacacg
     9301 atggcaaacg caccggccaa aatcatagcg gaaacttccc acagggcttt acggagaatc
     9361 aagcccgggc gagatgcagg agccccaaaa acccgaggaa ccagttgact ccctatgagt
     9421 gctttggcta cattaagccc gttcttgtcc ggcgggtgag gacgagtcat ccggattaca
     9481 gtcccgcctt catagatttt tccacgccac acttctcatc gccgtcgctt ctgcactgca
     9541 gagtgtgcgg gcggagaggc gtgattttgc ggagggacga tggatatcgc caaagtcgag
     9601 cgagtgtaca ccagttattc cggcgtctac gatcatattt tcggaaaaat cttccacgag
     9661 tcgcgcgaag cgtgcgtgcg caatcttaga atccgaccag aagagaaaat tttagaagtc
     9721 ggcgtgggaa ccggaatcgc cctggagtac tacccgaaga attgcgaaat catcggcatc
     9781 gacctctctt caggaatgct ggcgaaggcc agacagcgcc agtcgcacta tcatcttgac
     9841 catgtgcgac tgatgttgat ggacgccgga aaaatggact tcgccgacga cagcttcgat
     9901 accgtgatgg ccgcctatgt cgtgactgcc gtaccggatt accgcaaagt cgtgaacgaa
     9961 atgattcgcg tctgcaagcc aggcggacgc atcatcatgc tgaaccactt cagcaacggc
    10021 aacaaactga tcgctgccgt ggaaaaagtg atttctcccc tctgcaagca tatcggcttc
    10081 cgaaccgacc tctcgttgaa caccgtgctc gaagggacca acctccacgt cgcccgtaaa
    10141 gaaaaggtca acccgatgaa gttctggcac ttggtggaat gtgtcaacca gaagggtgcc
    10201 aagaacggca gcgtgaatgg acacgggagt gcgaatggga acgggaacgg aaggcacgga
    10261 catcacagct aacaggctga tttagtctca acctcatccg ctggcctgat gtgtgggtct
    10321 gaatgagttc cacttcaccc attcctcgtg tgagagaaac gtctgcccta caaggcacgt
    10381 cccgcgatac cgttgtgtga ccaggtcggt caccaggctc agaagtcgac cacccaagcg
    10441 accggcagcg tcacgtgcct gcaagcggag tccgaatccg gctccatcta ggggcccata
    10501 cgcctccacg cggagcgccg ctgcagcccc ggccgccatg tcggcagcca tcgctaactc
    10561 cccctcccaa cacatcagac tgtgccgacc ttgccgacgc gtcgctgcct tccagataca
    10621 gggcacaccc acctgatcca gccgggcaag cagccattcc accgtagggg ccacaccgga
    10681 atcggcctga aacgtccatt gcgcaatcac gcctcccatg tccgattcac tggccggcaa
    10741 cgagacttct tccagcgtcg cttcttcgca gactgtataa agattcccgt cgggatcgaa
    10801 ccagaagcgt aatttcccct cgctcatctc cacctgaatc gcccccagag aagagcagtc
    10861 gctgccgtcc tgctcaaaga gggaaagatc ggtgacgccc agcatggtaa gtttggcgac
    10921 ccgtagaatg ccctgcgtgt gataccgcac gaccaccgtc gccatactcc cagccatcac
    10981 ctcgcgacct gaggcttcgt cgaagatccg gcgcttggac aaatgcacat cggtaatgtg
    11041 cccgccgcga aaccccgccg tgcgcgccag aaaccaacgc agatcatcca ccgtcttgat
    11101 cgaatgggtc atgaatatcc cacggccttt cccccaggtg gcgttatccg accgccgcac
    11161 tctttccacc ttatcatgcc gggaccgtcg atgagcgcgt gaagaagaga acacaaggag
    11221 ggagacgacg acaagcggtc gctagaagag agcctgttgt tgcttgaact cggccaactg
    11281 acgctccaac tcatccacgc gttcctggag acgctgattg tcgaggcgca gcagatcgag
    11341 acgatccaac aattgttccc gttggatatt cgtccatttc agcgaccgcc tcaatccagc
    11401 cagctctagc tgagcctgct gtccgtcctc aaacccggaa cccatgcgta cgcctcagtc
    11461 gatcgccatc cggcagtaga tggcaaatcg taacgcactc tcggtccatt acggagtgat
    11521 tactgattta tcgacagaga aggtgagcgg aggggggatc aacagaattg gatgaataca
    11581 ccgggagcgt gagacgatca actcgcgcgg gcgcggatcg gcaatgggtt ggtcgaacgt
    11641 gtctctcctg cctcccagcg atagcgaaca agaacgccgt tagcctccca tcccggcggg
    11701 cacagcttgc ctgtgccata gtagtcggca tccagcacgg aaaagggcgc cacattcttg
    11761 atccagtcct gggcatggcc gttcgactcg atataggatt ggaagaaata cgcgccaccg
    11821 gtgagcggca actcaggcaa aatgaaatcg acataccctt cacgacgcaa ggtcaagggt
    11881 ttcggatcga cgatgtcggt ggataaaacg aacaggtctt gcgccttccg tccattcacg
    11941 gaaatgctga cgcggcaatt caaaaattct tttcccgccg cacaacggta gtacatacga
    12001 atcaccacat ctcgaccggt gatcgccgct gcgacaggat gcctctgagt atccatcact
    12061 tcgatcctgg tgagcaggat ctcgccgagt ccttcccgat catgacgcgc ttcgatcgcc
    12121 gtccccgcca gaccccgaat ggcatcggca tagccctcaa cgacctcgtg cgtgttgccg
    12181 atcttagcga cacgcccctt ctcgagaaga atcgcgcgtt cacacaaccc ttcgatcacg
    12241 gtcatgttgt ggctgacgat cagcaccgtc cgcccgcttc ggctcacttc ttccattttt
    12301 cccaggcact tctgctggaa actggcgtcg ccgacggcca gcacctcgtc cacgatcaga
    12361 atctcaggct cgaggtgtgc ggcgactgcg aacgccagcc gcacgtacat cccgctcgaa
    12421 taccgcttga ccggtgtatc gatgaacgct tccacacccg agaacgccac aatttcatcg
    12481 aactttcgcc gcacttcccc tcgtcgcatg cctagcatcg cggcgttcag gtaaatgttc
    12541 tcgcgaccgc tcaactcgga atgaaacccc gtacccactt caagcaacga gctgacacgc
    12601 ccgtaaatat ccgccgttcc cgtcgtcggc tgagtgatcc tggaaaggat cttgagcaag
    12661 gtgctcttcc ccgcgccgtt gtgcccgacg attcccagca cttcacctcg cttaacctca
    12721 aacgagacat ccttcagcgc ccagaacgaa gggttcgact ccggtggacc accacccaat
    12781 cgcgtcaggc ctcggaggcg atgcatcaga tgatcccgaa gggtattatg ctgacggctt
    12841 gcggctccga gccggtagtg cttcgacagg ccgtctgctt tgatggcaat atcgctcatt
    12901 caaatcacgt ccgcaaacgt tcgttccatc gaacggaaat agatgaggcc cccgacaaag
    12961 acgacgaaca ccacacctaa acttggcaag aacatcgtaa aatcgacctg aaacccggga
    13021 ataagcgccc agcggaagcc ctcgaccaca ctcaccaccg gattcatcgc atagagtgct
    13081 cgccaggact ccggcacctt gctcacgggg tagaggatgg gcgacacgaa catccacaac
    13141 tggatcaaca tcggcaggat atgcccgaca tcccggtact tcacgtacag cgcggaaatc
    13201 cagagcccgg cggccagcgt aatcgtggcg gccagcgaag cgaacagcgg cagggataga
    13261 atccgccagg tcgggaggat tccaaaccag gccatgaggc ccagaaacgc gacgaatgcc
    13321 aatgccagat cgaccagagg cgcgatggtg gccgaaaggg gcagaatcag acgcgggaaa
    13381 tacaccttgc tgatgagcgg cgcttctccc actaaactgg ttcctcccct gctcaacgcc
    13441 tgagacacat agaaccacgg cagcaagccg gcgtagagaa acaccgggta gggaaatccg
    13501 tcggatggaa tttttcccat gacactgaat acaaccgtaa aaatgagcat ggtcgcgacc
    13561 ggctgaaaca tcgcccaggc gactcccaac agggtttgct tgtaccgcac ggtcacatca
    13621 cgccagacga gaatataaat cagctcgcga tactgccaga gcgcggccaa atccaaatcc
    13681 agtagaccgc gggctggctg gatgcgcacg tgcggtaagg tcgacaatgg cctctgatcg
    13741 gcagtcaatg cggacatggc aatttcgtca ctcctcatga tcctcacacc gacggcaatc
    13801 gtaccgaagg ccagcgccct gagtctcaaa agcaggaggg cctaaccgcc agtttacact
    13861 ttgtcctaac tcgaaccgac tgtgatcgtt tatgtcaaaa ttttccgctt tggtcaagaa
    13921 attcgaactg ttatgcactt ctgtggcccc agccctgcat ggattgccga atccccgcgc
    13981 caacgtcgag caggcgggag ttgcaggaca tccacctgga caactccatc acctcaattg
    14041 tcgggcagta ccctgaagaa tcaactatac caaatgactc tggatgagga acgatacagg
    14101 gttgggagca gtcacgtgta atagggaatg atcggctacg gtgcaggagg gacaggtttc
    14161 ggtgcagcac gcaattcgtc ggcagagacg taccgaatcg ccaacccgga catgtggagc
    14221 atccacccct gcccgccgcc ggcgccctgc gtaaaactcc acgcatccat tcgaaacgga
    14281 ttccagtagc cgctgcgata ggaattgccc gacgtcgctc ccggtaccgc cgtgcccatg
    14341 gtcgattgga atgtgggccc ccggtctgcg aggcgccgca cgtccgcctc cccaaggaca
    14401 acggcatcgg ctcccagctc tttcgcccgt gccaggattc gatcacgcaa cgtatcttca
    14461 ctggcatatt cactggtcgc aatgatgcgg gcgatctgca cgtggccgtg atgaggctcg
    14521 cgctgcagcg tcgtcacgtc ctcgcttcca ggtcgaggct cgaacgcctt gctggtcaag
    14581 ggatctacct gcacggagac acacgcactc gagagcccga gaacaaggac caacgcaagg
    14641 cgggaggcct gcttcatcat ggctcgattt cgaatgttca tgtccaacag tccaggatca
    14701 aatctacttt agcttcatcg agttctatag tgacggcttg gatcgactta ttcaacccca
    14761 aattgctcca agattttatg gtacccatcg ccgaccctgc ggtcaatccg gaaactctgg
    14821 tatcttattc ttcagtgtca tgctttgcac tacaaccggc cttcgacgac cactcgaatc
    14881 tcttccatac gccgtcgatt cacacccaga tcgctatagc ccgtccggga agccgaccga
    14941 aaatggatcg tcttcgtcgc atcatcaaac acgaattcga catcgtccac gaatcgaagc
    15001 agcaggctcg taaattcgta atggaggtag gcctcgtcct cttcaacgag tttcgcacgg
    15061 ggtagattgg ccacaatggt tttgagcgtc tctttggccc ccgccagagt tttctggtat
    15121 cgatacggca cgattccatg cccatgatca gaggcctggg tcgacacaca attcggactg
    15181 gatgggcagg gctggagtga tctgatcatt gcgttatcaa caatatatag tgcctacggg
    15241 cacgggctcc aagccggaga cagctgacca gatacactac ccccaatatc ccaacgaggc
    15301 ttaacacctg attattgaac tgtaatgaga ggcccctccc ctgtcaaccg ctgtaccaga
    15361 ggtctggcaa gctcctgcct atgggcttta gttgaggtga gcaaccaacc aggcgtgaca
    15421 ccggggtacg gcagccatcg ccggttgttc aatcaggcga aacgtggcaa aacacagcag
    15481 aatgagaacg ggcacgaggc ctagcacaat ggaccaatgt tcagcggccg agagagttga
    15541 cgcccattcg ccaagtacca accgataggc ggcaaacaac aacaggctat gcagtaggta
    15601 cagactgtac cccatttcgc tcaacaggac agacgtcggc cattcaagaa tgccgtagat
    15661 tgagttccca caggcgataa tcgtgaacgc gccggtcagt agcatcaacg cgggaaatgt
    15721 ataggccgtc ggaaagagcc acatagttat cccaagacaa gcgaccacga ccaaccccca
    15781 gactcgatgg gcgagcctcg tacgtatggc atcaacacgc gccaggctcg cagccgcgat
    15841 ccctccaccg aacgcataca acacagggcc gcgaacgtcc ggcatcaccg tcgtgagtgt
    15901 cacgactgcc gcggcgctga acagcagcca cgaggtcggc gtcgccgcac gaaggcacca
    15961 tgagccgagg ggcaggaatg catagaacaa ccattcatac ggcagagacc acacggcccc
    16021 ggccaaggga atcgtttcgg caaagccatt gacgggcgca atcccaggaa tcgtaaacgt
    16081 gagccaactc gcgaggtgca gcgcaaagga ggtcatcgat tcctgcagtc gaaatccggc
    16141 acgatagagc gcgatactca ccacaccgat caccatcaat agatacaacg gcaccagtcg
    16201 aaatatccgg gaaaggtaca gtcgcgacca gtcgaccggt tgcactcgcc cgtcgagtag
    16261 cttggaccaa aagagaaagc cggtaatcat gaaaaagagg gtcacgctgc tttgtcccaa
    16321 gtgcgtgtaa aaattagacg gagggacatc ccacgtgccg gaacgcagat agaaatacca
    16381 gacggaagaa tggctgagaa acacggcgaa ggccagatat ccccggagtc catcgataca
    16441 cgaatatcgg gctgccgatg tagttcgaag atggccgatg tccggaagaa gtgtggcagt
    16501 gcccactgca agcaagagcg ccgcgacagc aggccacagg ctggtagcat ccatgttatt
    16561 ggatgacacc ataggagttc acatttgtct agcctttccc gcaacctcac cggcgtatgg
    16621 cttccgacta tacgagtccg gctcccagcc cctcgcccaa ccaccaccgg gcatcaagcc
    16681 tcttcccata gcactccgtt gattgccgga gtgatcctgc ggtaccctag ctccttattg
    16741 atcccaggtt gagggcgaac agccgctcac cgattgcaca cagctgagcg ctgacgctca
    16801 ggtcaacaaa ggagtcctca cgcatggccg gtttcccgat cgaagtcgca acccgcatta
    16861 agacgctgcc gccttacttg ttcgccgcca tcgacaagat gaagcaggag gcactcgcgc
    16921 ggggagtcga tatcatcaac ctcggtatcg gtgaccctga tctccccacg cccacgccga
    16981 tcatcgacag cctggcccaa gccgccaaaa acccgaagca tcaccaatat ccctcttatg
    17041 aaggcatgct gtcgtttcgt aaggccgtcg cggattggta caagcggcgc ttcaatgtgg
    17101 cgctcgatcc cgcggatgaa gtgctgacct tgatcggatc gaaggaaggg atcggccacg
    17161 tccatctggc atttatcgat ccgggagaca tcgtcctcgt gcccagccca gggtatccgg
    17221 tttatccggt cggaacaagc ttttgcggtg gcgtctccca catcatgccg ctgacaaagg
    17281 ccaacggctt cctgcccgac ctcaacgcca tcccgaaaga agtagcgaag aaagcgaagc
    17341 tgatgtggct gaattcaccg aacaatccca cctcggtgat catgagcaag gactacttca
    17401 agcgcgtggt ggaatttgcc caggataacc aggtgatcgt ctgtcacgat gcggcctact
    17461 cggagattta ctacgacggc aagcgtccgg cgagttttct cgaagtggac ggggccaagg
    17521 atgtcggtgt ggaatttcac tcgctttcca agacctacaa catgaccggc tggcggatcg
    17581 gattcgccgt cgggaacaaa ggggtgttgg ccggactggg ccgcgtgaaa agccagttgg
    17641 actcaggcgt attcgaagct gtgcaagcag cgggaatcac cgcgctcggg ttggacgatt
    17701 cggtcaccga tggactgcga aagatttatc aggaacggcg cgataccctc gtgcccgggt
    17761 tgaagaagct cggcctggaa gtcgatccgc ctccagcggc cttctacatc tgggtgacgg
    17821 ttcccaaagg ttatacctcc gcctctttca cagcccatct cctggagaag gccggtatcg
    17881 tcacgacacc tgggaacggg ttcggtgccc cgggcgaagg ctacattcgc atgacggtgt
    17941 gcacgacgaa ggaacgactg gcggaagcag tcgaacgaat caagaaagcc ggattctaag
    18001 gcaagcgtga cggcccctct tccattctga gaagaggggc ccgatacgaa tatcctcatg
    18061 tgccattgag ccctgtcagg acaacgatgg ccacagcatt catcggtttc ggctctaacc
    18121 tgggaaaccg gatcgacttt tgtgatcggg ccgtgacact gctcggcctg ctcccacatt
    18181 cacggctgga ggcggtgtca tcactctacg aaaccgaacc gctcgaggat ggggccgccc
    18241 ccggcccctc ctggttcctc aacggtgtgg tacagatcga aacgaacatc gccccgaaga
    18301 gtctgctgga aatctgccgt gagatcgagc gggcgctagg gcgcgatcaa gacaaccgca
    18361 aggggcctcg caccctggat ctcgacgtac tgttgtatga tgactgcatt ctcgccgagc
    18421 cggccctgac cgtcccgcat ccccgcctgc atcaacgacg atttgtgctc accccgttgg
    18481 tcgaactcga tcccgagcga cgacatccgg tgctcggaca aaacctgcgc gatctcttat
    18541 cgcaactcac tgatccatcg atcgttcgcc tgctcgagcc ccaaccgaac tcccgctatg
    18601 gatcacgtcc aacctgcagc gcacccccaa cagcagggcc acaagcgtga agatcattcg
    18661 cgcgacgccg gccatggccg actggagccg ccgactccat cgagaaggca tcacgatcgg
    18721 gttcgtgccg acgatgggag ccttacacga agggcaccgc gccttgatcc gtgccgcccg
    18781 actggcctgc gacgctgttg tggtgagcat ctttgtgaac ccgacacaat tcgggccgag
    18841 ggaagacctt tcaaaatatc cacgccagct tcgacgtgat caagcactgt gccgaaagga
    18901 aggggtggat gtcatttttg cccccaccgt caggacgatg tatccgaccg gcgctcaaac
    18961 ggttgtgact gtcccgggga tagcccggcg ctgggaaggg gaagcgcgac cgcaccattt
    19021 tcaaggcgtg gcgacagtcg tcactaagtt gctgtccctg gtccggcccg acaagacgtg
    19081 gttcggacaa aaagactatc agcaagccgc cctggtgcaa caactcgtga aggatctcaa
    19141 cttgcccggg cttcccattg tccatccgac cgtgcgggag gccgatgggc tggcgctcag
    19201 ttcacgcaat gtctatctgt cgagttcaga gcggcaggcg gcgcctgtgc tctctcgcgc
    19261 actccaggcc ggcgtagcgg cgattcgtgc cggagaacgg aatggaacgc gtatccggcg
    19321 ccacatgctg cgcgtggtcg cgacagagcc gctcgcaact gtggagtacc ttgcagtttg
    19381 tgatcctgcg actctcgagc cgctttctct gctcaaccgc caggctgtcc tactcggggc
    19441 catccgcatc gggacggtac gcctccttga caatgtgctg acgtcattcc gccgcaagcc
    19501 ttaacatcga agcttcacgc gcaatcgcga cccgacgccg gaaggaggcg ggagtcacgc
    19561 gaatgccacg aagttgaacc gtgtggttgt tatgcgctga cgcggggagg cgcttgttga
    19621 tgtgcgagga gctcgaggac cagctgaccc atacgctgct tgtcatcagg ttcaaactcc
    19681 aggaacttca ccccgatcga cagcggctgg atggagcaaa tcatagcggc gtcgactttg
    19741 atatcgggct gtccggcagg agcatgcacg atcagtttca cgaacgtacc ccgcgggagc
    19801 gaggaggatg cttgcaacgt acagccgccc attgagatat cggtgatcac ccccccgact
    19861 gccggctggt ccaggatcag cgacgcggcg aagttcgccc gcagccggcg gtactcacgc
    19921 cgatcaaact cttgggaggt ctgccggtta cccggccggc gggcctgaaa gcgagccgtg
    19981 cagagctgac aacgaacttg aaagatggac aagcggttca gtgtcttctc catcaaattc
    20041 gtcccggatg tcactctgac acagggcgtg ccgcacgccg gacaatacag ctctttcatg
    20101 ctacgactct gactcctgca ttcttgtgtc cacttgcgat tggtccgcca aactcccgga
    20161 ggattgcacc gcaagcagcg gtttcacctg ggccggagaa taggtcggcg gttttaccca
    20221 cttgccatcg gctcgcttgt agccacccac tttgctcaaa ttcgaccgat gcacctcacg
    20281 aaacaccgga tccatatcca atccatagga cacggctgtg ccatagacca catacaacag
    20341 atcggccagc tccttcgcca ccccggggag atcctgggcg accatggcct cctgcagctc
    20401 ttcgaactct tcttgaatca gccgcacccg aagctgtcgg gtcgcttctt ccggaagaga
    20461 aggccggtcg ctgacggcta tatcgaactt gcggtgaaac tcttccacca tcgcctgcgg
    20521 atccatcatc ctaacgtccc tctactctct gccgcaaacg caactccggt ccttggttcg
    20581 atgccggagg cttccagtag cgcaatgtct ttatcagatg atatcccgag gtctttgaat
    20641 tttctagctg ccggcagcac gcgagactcc aaagatccga ccgccttatt gtaggccccc
    20701 acgcttttcc cgagcgcctg gccgatctcg ttcaggtgat cggtgaggac ggccatccgt
    20761 tcgtacaatt ccttccccag tcgtcccgcc tgctcggcat gttcgttcaa ctgttcctgt
    20821 ttccagccat aggccaccgc gcgcaagagc gccatcagcg tcgtcggcgt cgcgagcacg
    20881 accccttgcg caaacccgtc ttcgatcagc gtcggatcct gctccaacgc cgcaccgaga
    20941 aactgctccc ccggcagaaa aaggacgacg aattcaggcg tctgcccgaa ctgcgtccag
    21001 taggccttga gactgagcgc gtccatcctg gcccgcacct gaccggcatg gcggagcaga
    21061 tgcgcttgac gctgccggtc atcctccgcc tcataggcat cgaggtaggc ggctaagacc
    21121 gttttggcat ccaccacgat ctgcctgccc cccggcaggt acacgaccat gtccggacga
    21181 atgagccctt ccaaggaccc gatcgtatcc tgctccacaa agtcgcaatg ggccaccatc
    21241 cccgcaagtt cagcgacgcg acgcagcgtc atctcacccc atcgcccacg cacggacgga
    21301 gcccgcaagg ccttcaccag attgccggtt tcctgttgga gccgctggtg cgattcagcc
    21361 atgaatctca actgctggtc gagtcctcca tattcccgct gccgaacctg ttcagcctgc
    21421 tgcagctgtt cttgatatcg ctggagcgat tcctccaagg gcttgaccag ttcgccgatg
    21481 gcttgctgcc gctgcgacaa ctcgcctttg gcctccgcct gcaaggtggc aaacgacgtg
    21541 cgcgcgaggt ctaaaaacgc ctcattgttc tgcttcagtg cctggccaga cagggcctcg
    21601 aatgagtcat ggagttcttg ccgtgcctgc gccagcagcg ccttctggtc ctccaaactg
    21661 cgggcgacct cttccatgcg ggtttcggct ttggtccggc cctgctgcga ctcggcgagc
    21721 gcgtcgcgca actgccccat ctccgactgc tgatcatcca ctcgctgctg cagcgaagcc
    21781 gccacagcat cggcgcgctg cgcgcgctct ccgctctcaa gcaaccgggc atggtggcgt
    21841 gattggacac gaacgatcac ccaccatccg gccaccagaa cgcccacggc gagaccaacg
    21901 gcggtgccga ccaaaaacgt tggggaagtg aggtcgatca tggcgcttcc ctacgaatgc
    21961 accgactgac tggaaatcca ttcacctaac tgttgtaggg aaatcgcgta gagggtacga
    22021 gaatccagga gaaacgtcaa gagaccaatc gactgatgcg gcctgtggaa cttgcgattt
    22081 cgacctgttc attttcgcgc aatagactag tggcataggg cacattgacc aggaccggaa
    22141 ggccgtattc acgagcgatg atcgccccgt gcgagagcgt ccctcccatt tcggcgatga
    22201 ggccggccgc cattccgaat aaaggagcca taccgggatc gatcaccgga acaacgagaa
    22261 tgtctcccgc tcgcacccgt gcccaatccg ccgtcgaccg aacaagccgg accggtcctt
    22321 tggcaacccc agcactgatg gcaatacccc gtaacacgcc tccctgcccc tgatcgcttg
    22381 actgcgcccc ctgctcgacg accgcctccc aatcccgaat cgtgtcgggc acctgcaggg
    22441 cctcatgctg aagccgttcc tcgcggcgcc gacgcacgag gctctgccaa tcacgacttg
    22501 cgccatcagt cagtgcgatg cgttcatcca gcgtgagata aaacacatct tcccgcacgg
    22561 cgaagcttcc gcgtgccacc atgagctcac cgagccgaag caacagatgg cgggctgctg
    22621 tggagtaata catcaggtga tggcgattct cctctcgcaa ggcacagaag cgactgagcc
    22681 tccgatacca ccaacggaac acgagccaac gatggcgtcg ccacccgaat cgccgggcga
    22741 tctcactgag cgcctgttcg cgtcgctgag cctggcggga aagaacttct tggggtggcg
    22801 ccgtcacgcc ggcgcgcacc tgtgcccgca acagggccaa cacggcatcg ggctgatctg
    22861 caattcgtgg ggacatgata tcggactctc cgactgcacg atgcccgtac tcggctaagt
    22921 actgatcaaa cagccgcagg aactccgtcc cctgaatcgc gtcccgataa ccacacgccg
    22981 cccactcctc cgagaaaaac cattgtcgaa ccgtttcttc ttgctgtgca gctacgacaa
    23041 gctcagccag ccggacaatt tgggaggcac tgatgacggt cccctgtccc tgcaatgccc
    23101 cattgagcaa ctctcgccag tctgaaccaa gccagcccgg aagcaccgtg cccatagcct
    23161 gaagactctg cgccactcca cccgcgattg cgaaggtcac ctcatgatcg tcgagccacc
    23221 gccctatgct gtcgagaatt gcggccaatt cctgcaccga cagattttgg atgcgatcat
    23281 agcggtagcg ttgcgccatg gccttcatgg cagaaaaatt cttcggcccc tgcttcgcgg
    23341 ccttacgcca ctcccgcagc ataccgactc cggcacgaag aagcgcgagc gccccgagag
    23401 gccgtaccga cggggtaaac atcaggggct caccacccat ctgttcggtt aagaacgccg
    23461 gattcccatg aagctgcatg accaaggtgt aaaacagagt cacgttgaga tagggacgtc
    23521 catggaacac acgaaccgac gagagccctt cggggaccgt gcaaccgagt cggcgatagg
    23581 gggtgatgat gtaggcatcc atgaatcgct cgagaaacga cagccctaac gggctgggca
    23641 attccggcaa ggtttctttg aaattagccc gcgtccactc acattcgtca ttggtcagag
    23701 agggccacgg tcgaacggcg gtaatcggac gggcctgcac gatccagagt cgctccacat
    23761 ccaacaccca ttcgagatcg accggttgac gaaacgcgac ttcaatgcgc ttcgacaacc
    23821 gtgcgagctc gaacagctgc ttgtctgtca ggcaggcctg ccgttgttcc ggttcgggaa
    23881 tcgactcttc ccgcacacct ccaggggtgg cggtcagctt attccgtttc cgggcaagca
    23941 tgcgtcgtcg tacgcggaaa ggccgatgat cgtcatcgta tacctccacc acatactgat
    24001 ccggcgtcac cactccattg accagggaag aagccaatcc tggcacggca tcaatcgaca
    24061 cctgagaggt tcgacccgtg acggggtgaa tagagtatgc gacccctgct gcctgtgcgc
    24121 tcagcatggg ttgaatgacg actgccatgg cgggacaagg ccggtcggca ccggaacgaa
    24181 gaaaataccg aaacacccgc tcgtcccata acgaaatcca acagtcacgg atagattgca
    24241 gcacggccgc caggggcacg cccagggtcg tgcgatacag accggcagcg ctcatatccg
    24301 cagcatcttc attcgtggcg gaggaccgga cggcccagcg tgtcgaggaa tccccggcca
    24361 gccctgtcag tcgtgtttcc aggtcggccg ggaatcctgt cggccagggc tgctccagca
    24421 agagccgctg aattcgtgcc agctcctgtg cccgttgtcc ttcagacaag tggggtagct
    24481 tcgtccagag tgccaccgca tcgacaccag cctgatccag gccgtgttga tagactgcag
    24541 tcgtcacaca cagccccttc ggcacaggaa ggccagcaat gagtaccttc gccaggcctg
    24601 cagccttgcc gccgacaaga aagggagcac agctctcata cagagagagg aggaacggat
    24661 cagccataga gggggatact agctagagtg cgaggaggag gtaaagatcg ttcacattgg
    24721 tcccggttgg gccagtcaca atatgactac ggagtttctt gaagaacgga taggcatcgt
    24781 tgccctgaag cgcatcaggc ggatcaagct tggcgcgccg ggcccgcgca gtcgtttccc
    24841 cgtctaccac cgcgcctgcg acggaagtcg gtccgtctgt gccgtcagtc gcaaaacctg
    24901 ccacccacac atcagacaac ccggcaatct ccaatgccgc cgccaaggcg aactcctggg
    24961 ctcggccgcc ctttccctgc cctcgaatcg tcaccgtagg ctctcccccg gcgatgacac
    25021 aacagggacg agccaaaggg cgaccatgcg tcgcaatctc ccgagctatc gcaccgaaaa
    25081 acttggcgag ttcgcgagct tcccccgtaa cggtagtgga aaggacaagc gtctgtagcc
    25141 gcttcccctt cgcggccttc acgacggcat cgacagccgc ttgattgtct ccaatgagca
    25201 gattgtctac acggcgaaag agtacggcgc cggctttggg agtttcgggc agggtcttct
    25261 tcatgcccgc ctccaggcgt cgacgcaccg aagctggaac tttccgggca acctcgtatc
    25321 gctccaaaat tctccaagcg tcccgaaatg ttgtggggtc aggagcggtc ggaccagacc
    25381 cgatcgtacc aagatcgttc ccgatcacat ccgagaggat cacactcgcc acacgcgcgg
    25441 aggtcgctgc ggcaagctgc ccgcctttta cacaagacaa gtgcttgcga accgcattca
    25501 tttcgtgaat ggtcgctcca gaccgcaaca gcagcttagt cgtcagctgc ttggccttga
    25561 gggtgacccc aggagcgggg gagggaagga gactcgaggc accgccggac aagaggacaa
    25621 tcagcaggtc gtccgatttc agcgtcccga ttaatgccat catcgcacgg ctggcctgga
    25681 gccccgcctc gtccggaacc ggatgcccgg cttccaggat gcgaattttc ttcgtcggag
    25741 caccatgccc gtatttcacc acgaccagtc cggtgtcgat cctcgccccc agctgccgct
    25801 ccaacgcctg agccatgcga cccgacgcct tcccggcccc gaccactaca agtcgctgat
    25861 gttgtgaaag aggatagcgc tccgaaccga tagtgagttg cccgcgccgg aagcggacct
    25921 gacggcacac cgcctcgtag gggtcgacgg cctgcagtcc cgcacgaaac agtgttgtga
    25981 agagggctcg tgccctgagc tgcgagactc cgacttgtat catgggcact acgggagctt
    26041 ctcttgcttg aaacagcgat tcggacaggt ttgtcgcggc accttaagct ggtaggtacc
    26101 ctttggcaag tagtccttcg tgaattcggg attcagcatc ttcaattcaa gaaaataggt
    26161 tccgtattcc tcagcgatcg ccgtgaggcg gcgttgcggc tctttgatgg taaccgtcac
    26221 cgtttctgtt tcaaggggag ggtagagatc cttcttggtc agaccaagat acttctccgg
    26281 ctgggaatag atctctttcg ccgcaataat gcgtgccaca taccgcatgg tctctcgtgg
    26341 gccgtgcatg cgccagtagt cattgatctt ctgttctttg atcaatttcc gcacgcggtc
    26401 ttccccggcg ttgtaggacg ccatcgccag gaaccaatct ccctgataca gatcccgcag
    26461 ataccgtaga tacttgatgg cggcttcggt ggacatttca agattgcggc gttcatccag
    26521 ccagcgttcg ttatggagct tataccgtcg ccctgtggaa ttaatgaact gccagggacc
    26581 tgatgccttg gcccgggaat aggcggccgc tatacacttg ctctcgacca ataacatata
    26641 tttcaagtca tccggcaatc cggcctcagc caactgcttt tctgccggcg caaaacatcg
    26701 acctgtccgt ttcgccagaa tgatactttc accttgatcc tcgaggaact gatagaactc
    26761 gtactcgatc cgctcgcgta cctgccagtt atccaacgga atcgcctgcc cggcgaacgt
    26821 cagcttatcg ggcaacttga aggagctgag gaagaagcgc tctccctccc gtttgatttc
    26881 ggggaggatg accagtcgat cttcaagctc aggcaccaac tccgactcgc ccggcggcgc
    26941 gaccggcacc tgatcagact cctgggcgtt ccggccctcc tccgccttag cgccggtcaa
    27001 cgcggcagaa ctcgaatccg ccgataaaac cggcatggcg gaagacaggc cgaacagcat
    27061 tgtgacgcac gctagaaatg cctgaacacc ccgctctttg ttccacatgc ccctgtacat
    27121 cctttcgtcg cgactgatgg ctcatgctag caacaagctc gccgtgggac aagcatctcc
    27181 tggttttggt acacttcgcg cctatgtccg ccctgacaat gctcaacaac gagatcgtcg
    27241 cctgcacgtg ctgtccccga ctggttgcct accgagaagc tgttgccagg gataaacgac
    27301 gccaattccg cgagtggtcc tattggggta agccggtccc tggattcgga gattccaacg
    27361 ccaggctcta tatcttgggc ctcgcgcccg cggcacatgg ggggaaccga accggacgcg
    27421 tatttaccgg agaccgtagt ggggattggc tgtatgaagc gttgcaccgg ttcgggttcg
    27481 ccaaccaggc gacatccgta catgcgggtg acggcctcac tctcaccgac tgctatatcg
    27541 gcgcgaccgt tcggtgcgcg cctccggcaa acaagccgac gccggatgaa ttcacggcct
    27601 gccggccatt tgtcctgcgc gaactacggc tattgaagcg aatgcgtgtg atcgtcacct
    27661 tgggcaagat tgcattcgat cactatctca aggcgagccg tgaattgggg ctaggaactc
    27721 ccgggctacg ccctcaattt ggacacggag tcgtctacga cttaccttgg ggcgtgacct
    27781 tgatcggctc ataccatccc agccagcaaa acacgttcac cggcaagttg actcgaccaa
    27841 tgttccatcg catttttctc aaggcccagc agaaactcgc cgaggcacca ttgcgcgatt
    27901 agccgcttcg agagtctatt tctttcccat tgcatcccaa tcggccagaa acttcttcag
    27961 cccactgtca gtcagcggat gcttcacaag ctgctgaaac acggcgtaag gcatcgtaca
    28021 aatgtgcccc ccggccaacg ctgcttcgac gacgtgctgc ggatgccgga cgctcgcgac
    28081 gaggacctgc gttttataat cgtagttctt ataaatcgtg acgatctggc gaatcagtgc
    28141 cattccatcc gaactcacgt cgtcgagacg accgatgaat ggcgagacac accacgcgcc
    28201 cgccttcgcg gccagcaagg cctgcgtcgg ggagaagcac aaggtgacat tcacacgaat
    28261 cccctctgcc gcaagcttct ttgtggcccg aagaccttcc gggataaggg gaaccttcac
    28321 cacgatattc ttgtgaacct tggccagatc tcgcccttcc ttgatcatgg cctcggattc
    28381 gacaccaacc acctctgcgc tgatcgggcc atcgaccatc ttacagattt cgagaagcat
    28441 ctccttgaaa ctgcgccctt ccttggcaac aagagatgga ttagttgtca caccgtcgat
    28501 gaggcccagg ttggcaccct cttggatttc tttcacattg gccgtatcaa gatagatttg
    28561 catttgaaga tcctttcctc gctctgagca ttcgaaccca cttttcctca agttattgta
    28621 gaagcaactc gataccaagg caataagtca gggctgccgt gctagacttg tttgacagat
    28681 gagaatggtg aacgctaaga ttttctccgc aatagacaat aatgtcgcgc ttcacacccc
    28741 tgattcagga ggacaacctg tatgcgcaaa tccgcgtggc ttatagcgat cgccctcctg
    28801 tccggagcct gcagtcacaa tccataccta gatgcctctc tcactcgcta tcaaggaaaa
    28861 gatcgtgcgt ggattgaaaa ggaattgggc gctcctgatg ccaagacgtc ccgatttttc
    28921 ggtggagaga agtggaccta caatcgaatt gcggggggga aggcaggacc gccgctattt
    28981 aattttaaag caaacgaatg tcagttgatc ctgtttttta ataaggacga aatggtctct
    29041 gactctagct attccggctg ctagctagaa agttgcttct gaaatgtatc ggcgcaacta
    29101 cgactgcaga aataataggt ctgccctcca atctcctctc tcaccgcatt gccacgaggg
    29161 atgaagacac ggcacaccgg gtcttgaatc atttgatttc cagccgcctg ctcttcttga
    29221 aggctcaccg ccccgccatc ctctttcatt tttctaattg ccctgcgaag caggtagtag
    29281 aggagcacga gcaaaccgga aaccagcaat aatctataca tggtagtgca tcctgccagc
    29341 gccactaaat attaggccat cgtccatgac gtgaaaacag cgacgaaccc taccccgcca
    29401 tcttactagc atggaacaat cccgtaaggg gcaatcttag gaccaaatgg cccagatgct
    29461 tttgtgtcta tgaggggcct tttcccatcc ttatacagta cttcagatgg atggtttatc
    29521 ctacagcgca atgttataag ccaggaatgc ataaatgtga caaatgccgc ggaacaatgt
    29581 tgccggaacg ggcggttgac ctgaatgctg gcttggcgat cacagtcctt gcgtgcctca
    29641 actgtggccg acgaaaagca gctgacgcag aaccgagacc gatcacgtca cgacattaac
    29701 ccgtcgcaat aataatcctt cacaggacgc tcacatgcgt tgagccgaag ttggtttacc
    29761 ccaacttcct ctggcatttt gtccactctg gacttgatgc ccctctcatc gcagagcaat
    29821 cctccacttt atcctactag ccaatgcgtt gagctaactg ccaggtccgc ccagcttttc
    29881 tatccaatcc ccaagatttt acgtttctac atattcacgc agtgatccca gcaacaaacg
    29941 aattctcata tcaggagcac aaaatcattc caggcgtctt ggaatgaaaa agaggtagcc
    30001 tcggagggat tttttacttg aggctcaata catccagcat gtcatagaga ccgggtggct
    30061 gcttcacaac ccagcgggcg gcacggaggg ctcctctggc aaatgtgtcc cggctactcg
    30121 cacgatgagt gagctcaatt cgttcgccca tcgttccaaa tatcacggta tggtctccga
    30181 cgatgtctcc ggctcgaatg gtctgaattc cgatttcgcc cctcttccgc tcaccgataa
    30241 gtccttttcg cgcataaacg ccgacttgac caaggtctcg attgactgcg cgagccaaga
    30301 cctccgccat tttgagtgca gtaccactcg gtgcatcctt cttcaggcgg tgatgggctt
    30361 cgatcacttc aatatcgtag tcctccccga gggtcttggc catttcggcg atcaccttgt
    30421 aaatcacatt aacccccaca ctcatattgg gggaaaacac gcaagggatc tgcttactca
    30481 cacttcggag ctcatctaac tctgacgatg aaaacccggt tgtcccaacc acaatgccac
    30541 gtcggtgctg cgccacaatc ttcaaatgcc caagagttgc cgcaggcgtt gtaaaatcca
    30601 ccacgacctc cccacgctcc atcaaactgg aaagatcgtc gacgatcggc acacctgttc
    30661 gcccacaacc agccacttca ccggagtctt cccccacagc atgatgcccc ttgccctcca
    30721 cagctcctgc aagggtcaag aatgcagaat cttttaccaa ggacacgagg cgagaaccca
    30781 tccgtcccgc agcaccagta ataacaacct tgatcatggc aacaaccttt accagcagca
    30841 gaccgactac accagcccgg cctctttcat cacatgcaga agtttttctc tattgtcggt
    30901 tcccatcggg cacagcggca gccgcatctc acggtcgatc ttgcccatca tatgcagcgc
    30961 ttctttcaca ggaatggggt tggtttcata aaagagggcc gtaaacagcg gatagagccg
    31021 gtagtgcatc gcccgcgcat catcaactcg accggctaaa aatgcattga ccaactcggc
    31081 catatcactg ggcaccagat tggccgtcac cgtgatcact cccttgccac ccactgccat
    31141 catcggcagg gtcaacgcgt catctcccgc cagcacggtc agacgctccc cgcaaagctg
    31201 tatgatttcg gatgcctgct gaaccgagcc gcttccttct ttaatggcaa caatcgttgg
    31261 gcacaccgtc aatcgtgaga tggtgcttgg catcatattt acgccggttc gcccaggaat
    31321 gttatagaga acaaggggta aatcgactgc ctccgcgacc gccttgtagt gcagaaacag
    31381 cccttcctgc gtaggcttat tgtaataagg cgtgatcagc agtgcgccac cggcccccgc
    31441 cgctttcgcg tgcttggtca gagcaatcgc ttcctgagtg ctatttgatc ccgttcccgc
    31501 gatcaccggg actcgtcgtc tggcaacttc gaccgtaaac gccaccacac gatcgtgctc
    31561 ggcatgtgtc aacgtggcag actcgccggt agtgccgcag gggacaatcc cttgcgtacc
    31621 gctggtaatc tgccattcga tgaggtcccc gagggacttt tcatcgagct taccgttctt
    31681 aaacggcgtc acaatggcga ccagagatcc tgtgaacatg ggaggctcca taaggaagac
    31741 agtaaatact gaaatctagg aaagaaacgc cggtatctgc tcgccatgca ccaggtcgtc
    31801 gtaactctct cgcgctcgaa ttatatggat ctgtccctca tgaaccagca cctccggcac
    31861 acgaggtcgc gaattataat tcgaggacat gacaaaccca taggcgcccg cactcatcac
    31921 ggccatgaga tctccggcat tcatttccgg catcacccga tctttcgcca aaaaatcgcc
    31981 ggactcgcac acgggcccga ccacatccac agtctgcttc gccgcacgcg cgacgttttc
    32041 atagaccggc cttatatcat gatgtgcatc gtacaggctg gggcgaatca ggtcgttcat
    32101 cgccgcatcc acgatcagaa accgtttggt ttcgccgtcc ttcgtgtaca acaccttcgt
    32161 gagcaacacg ccggcattcc cgacaatcac gcgaccaggc tccatgatca ggatacactt
    32221 caggtcacgc acaagcggga aaatcgccgc cgccaaatct ttcggttccg gaggggtttc
    32281 atctgaatac gtaatcccca ggccaccgcc gatgttcaga taacgaatag gaattccctg
    32341 ctcggccagg gtctgcacca tcgccaacac tttcttcagc gactcgacga agggggccac
    32401 ttgcgtcaat tgcgacccga tatgtgcgtg caaacccacc acctcgatgt ggctgaacgt
    32461 tgcggctgcc ttgaactctt cgatggcacg atccgccgcg atcccgaatt tgctcttctt
    32521 caaacccgtg gaaatatagg gatgcgtttt gggatcgatg tccggattga tgcgcaacgc
    32581 cacgcgcgcc ttgacgccca tggacgccgc gacatcattg atggcctgca gctccgcaga
    32641 cgattccaca ttgaacatga gaatatccgc cttcaaggcg tcgcggattt cctccgcttt
    32701 tttcccaacg ccggcgaaca cgatcttggc gggagacacc cctgctttca gcgcacgaaa
    32761 tagttctccg cccgacacga tatcgacgcc actcccttcc ttggccatca gacgaagcac
    32821 cgccaaattg gaattggcct tcatggcaaa ggcgacaatg tgaggaatgt tctgaaaggc
    32881 actatcgtag acacggaagt gccggacgag tgtgtggtgg ctatacacgt aacaaggggt
    32941 ccccacctct tttgcaatgc gcgagagcgg cacatcctcg caatagagct caccttcccg
    33001 atactgaaag tcattcatgg tggtcatcct cgatcaacaa ttctataccc gcctcggaga
    33061 atccgtccaa tagccacaac gccaaccggt cgaaaccacg accacaacct cgactcacgt
    33121 ctatcgcgtg cccatcgtcc tcagtggcgg agtcggcaac gggtcgggat ccggaggaat
    33181 cggggcttct tcaacggtca ctgcactcgg cgcgctgggc ctggaagcag atccccacac
    33241 attcgcgcct ggcgctaaca gcccttcacg tctcaactgc cgctcaatca ccggtgccac
    33301 accgacgtct tcagggggga tcggcgcacc caataccccg catccgccca gtcccacaac
    33361 aatccacact gcaccgacgg caccacccct gactcccggg ccacaggccc tgacgagcct
    33421 catcccttca acgccttctc aagctccttg atccgtgcct ccacacgccg tctcgccgtg
    33481 ccgccgatct gacctttccg gtcgatggcg ccccgcacgg tgaggcaact caggacgtcc
    33541 tcaccgaaat gggcggagaa acctcgcaag tctttcaagg tcaactgctg aaggggccgc
    33601 tgctgctcga gtgagaatcg cacaatctgg ccggtaatgg aatgggcctc ccggaacggg
    33661 acccccttcg tcaccaggta gtccgccaac tccgtcgcca gcattccgcc accttcggcc
    33721 gcttcagcca ggacggtctt attgaccacc agcctccgca tcaactccgt gcaaagcgcc
    33781 aacgattgcc ccgtcgtatc caccgcgtcg aacaacgctt ctttatcttc ctgaagatcg
    33841 cgattgtaac tcaggggcaa tcccttcagc agcgtcaggg tacccatcaa gtgtccatag
    33901 actcgacccg ttttcccacg caccaactcc ggcacgtccg gattcttttt ttgcggcatc
    33961 atactgctgc cggtgcagaa cgtatcgggc agatccacgt aacgaaattc ctgcgacgcc
    34021 cagagaatca attcttcgct caagcgtgag aggtgcatca taatcagcga caaggcactg
    34081 agtgtctcca ccaccgcgtc acgatccgag acagcatcga ggctattctg cgtcaccgca
    34141 gggaattcca gcagtgcagc tgtatatcga cggtcgaccg ggtagttcga tcccgccaac
    34201 gcccccgacc ccaacggcat cacatcgagc cgttgccgac agtcgtgaag ccgaccccgg
    34261 tcacgatcga acatttctac ataggccaga aaatgatgcg ccagcagcac cggctgagca
    34321 cgctgcaaat gtgtataccc gggcatgacg acatccagat gcgcccgggc ctgccccacg
    34381 aggactcgac ggaattcctg caattgaccc atcagcgtcg aaagcacatc gcgcaaaaat
    34441 agacgcagat ccagggccac ctgatcgttg cggcttcgac ccgtgtgcag ctttccaccc
    34501 agcgggccga tcaactcagt caagcggcgc tcgatcgcca tatggatatc ttcgtcctgc
    34561 ggcgagaagg gaaaccgccc gccatccaac tccaccttga cgaattgcag tccccgcacg
    34621 agctgcgcgg actcacgggc ggtgagcacc cccgcgcgtt cgagcgtcct gcaatgggcg
    34681 atgctgcctt gaatgtcata ggggtagagc cggcgatcac aggccaggga ggaggtgaac
    34741 tgctccacca accggtcggt ttgttcggca aatcggccac cccaggcctt gcctttgggc
    34801 aaggcacgac gggaccgttg cggagcagtg acagatttgc gggcacgcgc gccggcagat
    34861 tggggcttcg ccattaccgc gtcgacctct tcttgcgctg ggcccgaatc gccaaccgga
    34921 gcgcgttcag acgaataaac ccttccgcat ccttctgacg atacacgtcg tcttcttcga
    34981 acgtcgccat gtccaaccga tagagcgagc gcggagactt ccgcccaacc accgtgcagg
    35041 tccctttgta gagcttcact cgaacggtgc cagtgacatc ccgctgggct tcatcgagcg
    35101 ccacctggag catttcccgc tccggagcat accaatatcc gtagtaaatg agctcggcat
    35161 agcgggggat cagactatcc cgcagatgca gcacttcccg gtccatcgtg agtgactcca
    35221 ggcctcgatg ggccgcatgc agaatcgtgc ctccgggcgt ttcgtacact ccgcgtgatt
    35281 tcatcccgac atagcggttt tcgacgagat cgacgcgacc gacaccatgc tcgccgccta
    35341 atttattgag atgggccagc aaggcggccg ggctcatctt cttgccgtcc accgccaccg
    35401 gattgcccgc cacatagtcg atctcgactt ctcgggcctt gttcggcgcc cgctccggcg
    35461 agaccgacat cacgaaaatc tcttcagggg gggccttcca gggatcctcc aaaatgccgc
    35521 cctcataact cgtgtggaac aggttcatgt ccatgctgta gggtttcgcc ttagtggccg
    35581 tgaccggaat gccgtgcttc tccgcgtact cgatcaactc acgccgcgag cgcatggtcc
    35641 attcccgcca gggcgcaatg atcttgatct gcggatgcaa cgccatatag gtcaattcga
    35701 agcggacctg atcgttaccc ttgccggtcg cgccgtgaca gaccgcctcg gcgccttcat
    35761 cggccgcaat ttcaatttgg cgccgcgcaa tcaagggacg ggcgatcgag gtgcccagca
    35821 ggtagctacc ttcatagatc gcattcccgc gcaacattgg gaacacatgg tccttgacga
    35881 acacctcgcg gagatcctcc acgtagactt tcttcacgcc cagggactgc gccttctttt
    35941 tgatggcctt cagatcttcg ccctgcccca ggtcggcaca aaacgcgatg acttcgcagc
    36001 catagacctc ttccagccac ttcagaataa ccgacgtatc caggcccccc gaataggcca
    36061 ataccacttt tttgtacgat gaccgactca tacgactccc tttcacttac gtgctccgtg
    36121 caggtttctt ccgaggagcc tgaccagaat ggccttctgc atgtgcaatc ggttctcggc
    36181 ttgatcgaac accaccgact gcggtccatc caacacctcg gcactgattt cctcaccgcg
    36241 atgagccggc agacaatgca tgaccagcgc atcgggcttc gcgcatttca atagtcgcgc
    36301 gttgagctga tagggcgcca gaatcttgag acgcttggct tgctcccgct cctgtcccat
    36361 gctgatccag acgtcggtat acaccacatc cgcatctttc acggccacaa acggatcggc
    36421 gccgatttca atcaccgcac cggtctgccg ggcttcctcc cgggctcgat ccacaatgcc
    36481 ccgatccggc tgatatccga gcgggcaacc gacggcgatc gtcatcccca tcttcgccgc
    36541 cgcctcgatc agagaattcg tcacgttgtt gccgtcgccg acatacgcga tcttgatgcc
    36601 tttcagccgc cgtttctttt cctgaatggt caacaggtcg gacagggcct ggcaggggtg
    36661 attcaagtcg gtcagcccgt tgatgacggg aatgctcgcc tcgcgggccc attcttcggc
    36721 aatcgcgtgg tcaaaggtgc gcaggacaat cgcgtccaga taccgagaca ggacatgcgc
    36781 cgtgtcggcg acactctcgc cgcgcgacaa ctggatatcg cccatcggca acaccatcgc
    36841 ctgcccaccg agctgattca tcccggcctc gaacgacacc cgcgtccggg tcgaaggctt
    36901 ctgaaacagc aggcccaaca tgcggccctg cagcagagga tgggacaccc cgcgacgctg
    36961 cttcactttc agttgcgcag ccaggcgcaa caaccccgtg agctctgcga cggggatcga
    37021 gagcagttcc agaaaatctt tcccgagacc ggcccgggag gatgaccgat gcggacgccg
    37081 cgacatacgc gcccctacag aattaatggt ggcttgatgt agttcgctga ctcaacacct
    37141 gcgcaagcgc gaccagcaag cgatcgatct cgcgctccgt gatgatgagt gggggaacga
    37201 agcgcagaac ccgatcgccg gtgcaattga tcaacaatcc gcgcgccagg caatcggcga
    37261 caacagcttt cccatcgata tccaattcca cgccttgcaa cagaccaagt ccgcgcacgt
    37321 ccttcacgca acgatgccgg tccttcagaa cagccaaccc tttggccaac gtctcaccca
    37381 tgcgccgtcc ctggtccaga atcttccctt ccagcagcac tcgcaatacc gccagcgcgg
    37441 ccgcacaggc cagaggatta ccgccaaagg tcgaggcgtg ggttccagga gaaaaggcct
    37501 gcgccacagt ttccgtcgct agacaggccc cgatcgggac accaccgccc agccccttcg
    37561 ccagggtcat gatgtccggc tgcataccga actgctcgta gcaaaaaagc gtgcccgtgc
    37621 gccccatgcc ggtttgcact tcatcgaaaa tcagcaacac atcgcgctcc cggcagaggt
    37681 cgcgcaagcc ctgcatatag ccccgatcgg ccacatgcac tccgccttca gcctgaatgg
    37741 gctccaacag gacggcggcg gttttcgatg tgatcgcgcg ctcgacttcc gagagatcgt
    37801 tgaacgtcac atgcgaaaac cccggcatga gcggagcgaa tcccttctgc accttctcct
    37861 gaccggtggc ggtcaaggtg gccatggtgc gcccgtgaaa ggagttcgtc atcgtgacga
    37921 tctcgtagcg gtctgctccg tacttatcgt acgaatactt tcgggccaac ttgatcgccg
    37981 cttcgttcgc ttccgcgccg ctgttacaga agaagacttt ctgcgcaaac gaatgttcga
    38041 ccagggtctg agcaagccgc acctgcggct cggtataata aaggttcgat gtgtggatca
    38101 ggtgctgcac ctgtttttga atcgccagca ccaggtccgg atgtccatga ccgaggagat
    38161 tgaccgcaat gccggccaca aagtcgatgt actcccggcc ttccaggtca tagaccttcg
    38221 agccacgtcc ccgcacgatc gaaatcggct ggcgcgtata ggtgttcatc aagtactgtt
    38281 cggcattgag acgcaactca cccgtcggca tggcacacct ctcccatcgt gagtcggcga
    38341 agctaccata gggcacaggt gaaagcaacc gaacgcacgc ctgtcagcag ctcctggcgc
    38401 ggccggcggt ctgtttcgaa ccctgccgct tacggagggc cgcccttgac ggggagagac
    38461 ggcggatggg ataaggtgac cgtatgaaat cctgttcaca atgccgacaa gaaaaccggg
    38521 acgatgcccg cttttgccac caatgcggcg gcgccttcgc cgttgaaagc caggaaactg
    38581 tggtggaggc ggactcggca ccttcgccgc agacggatgc agaactctgg aaagccttca
    38641 tcggccccaa tgccgaccgc tatctcgaga ccttcaagaa attcaccggg ccgtccgggc
    38701 cgaaattcgc cttgacctgg cactggccgg ccttcgtctt tgagcccttc ctctggtttc
    38761 tctaccggaa gatgtacgtc tacgcgctga tctatgccat cggcccggcg atggcgttct
    38821 atatcaccca ggacctctcc gccgacatca tctggcgtgt cattgcaggc gccagcgcta
    38881 actatatcta tttctggcat gcaaaagagc agctcgccaa gatcaaaggc gaacgggcca
    38941 cgggtggaga agccagacag cagatgttgg gagaattagg cggcgtgcag ccgtacgtcg
    39001 tgtgggtcgg cgtcggcctc ctggtcctga agatcggcct ggtcgtcgcc atgttcaagg
    39061 acggaccgcc tgacgggtcg aagaatcctc ccgccaaacc ccaccccgcc agcgtcacgc
    39121 acgtctagca aaacaacgtt cacaagtcgg ctccagcttt ctgacaacag ctgtcacaaa
    39181 tctgaggcgc ttatttcagg cgggataaga agctcaacgg atcttttaca aactgaaagg
    39241 cagtggattc ccggatttca tctttcaaag ccgacaaacc aggctctatc accttcccgc
    39301 cttcactgat gagccagcac tgaagatcgc gtgaaaactg cactgcagtc tccagcaact
    39361 caatcgttgt ctccctcaag tcaaaacgga attggacttc tacaagagac tcaccctctc
    39421 gcaaaatcgt taaacacgac gtctcaaggc taccaagcac ctcaagatcc ttcgaccatg
    39481 tcttaccaga cggaagcatc ttttccacat cgcgcaacag cgtaaggggt agccgctgat
    39541 ccctccacca cacaacggac tctcgctgtt cctcagtgag aaccattcct gtacgcaacg
    39601 agttcgcctc tgcgaccgcc ttcggcacta actcgatttg aaattgccag acagccatta
    39661 cctaatccga accctcgggg gcggaatcca ccctccagct cgactcatga aacaccggcc
    39721 ttcgctattc ggacagacgc tgccgctgcc actcgatcca ggatacgaac cagtcgaaat
    39781 cctgctggta ggcggctcca ctcagatcag gcgcggcctg ggtctgctcc agttgcgtgt
    39841 aggtgcgcgg ccagttcttt tgccaccagg acttgagctc gtccgggccg tagggctgcc
    39901 cgttggggtt ggtgatgccg gctgccgcgc agagcccggc cacaagcggc cagtagcgat
    39961 ccttgccaag accgacgctc cggaaatgtt cgcggagcag gaagaccacc cagagttcgg
    40021 cgctgttctt cttgttatgc ttgaaaggct gcgtttgtcg cagtggaacc gggtcgaagg
    40081 ttttgatgac cgacgtgtaa tccggacctc cgtagctccc cgccgactcg gcaatcccct
    40141 gcagcatgtt ggcgagttcc acctcgacga ccggcgagcg ggccgggctg tggtcagggc
    40201 ctccggtact caacggattg gccaccgtca gcaattcgat caacggcttg agctcacgca
    40261 accgaacggc ggcggctttg agaatctgct cggactcgag ccagtaatcc ggatcgccct
    40321 tggaggcccg ttcccgaccg ggcggcggca acaccacggg aatccagtag cgcaccagca
    40381 caaagaggac gtattcaacc tcgccaccgg cctgcgcgac accagccagc gcctgactga
    40441 ccccgagcgc ccgccgaaaa aaggcttcga ccctgtcttc aacctgcagg cgccgtccca
    40501 ttgcgcgcca ccactcctgc aatggggtcc agtctgaggg ggaacgttcc gtcatcgatg
    40561 cgctccggcg tgaggagaaa ccggcggcat ggtactggct gcctcatctg attgcaactc
    40621 agcatgacgg agccacggcg cacttccgtg ggctcatccc gcttgacagg gcgaagggga
    40681 aatcgcataa gctgtcctga aaccgttcta tcctttcgga caaggagacg aggcacgatg
    40741 agcaaattgg ttctcattcg tcatggcgag tcgcagtgga atcttgaaaa ccgcttcacc
    40801 ggctgggtgg atgtccccct ctccccgaaa ggtatcgaag aggcgaaggc ggcagggaaa
    40861 aaactggcgg ggttcacatt cgatcgcgcc ttttcgtccg tactggcccg cgccaacgaa
    40921 acgttgcgcc tcgtcctcga agggatcgga cagaccggca ttcccattga aaaagacaag
    40981 gcgctaaacg agcgcatgta cggagagctt cagggcctga ataaggccga gaccgccaag
    41041 aagttcggag atgagcaggt gaaaatctgg cggcggagtt acgatgtacg gccaccgggc
    41101 ggagaaagct tgaaagacac agcggagcgg gtgctcccct attacgacag ccgcatcaga
    41161 ccctacgtgc tccaagggga aacgatcctc atcgccgccc atggaaacag cctgcgagct
    41221 ttggtgatgc agctcgagca gctgacccgg gagcaggtcc tggaactcaa cattccaacc
    41281 ggagccccgc tgctctatga gctcgacaac aacgggaagg tgctgtcaca ccggtatctc
    41341 taacaggaaa caggagggcg cggccgacag cctacacgca ctggctcagc aactcctggt
    41401 acttatccaa gggcaccaga tcaggcagga gcacgagcaa cttcggccga tcctccggtt
    41461 gaatcaaaca ttgattttcg agcagatctg agatttcctc ggaggcggcc atggcgccgc
    41521 cttcgtaatc ccccaccggc gcatagagtg cgcgccgttc ttccatttcg accaggtagt
    41581 cctcccactt caccgactca agcttgaacg tcgcagccca atccgcctga atcaattcca
    41641 gcacccgctg aaacaatacc tcgcggtact tccgtggaat gtgggcattg acggccgcat
    41701 gaacccagta gagcgtgaaa gagaggagct ctcgcgtcat cgtctcctca tgttcttcct
    41761 ggccctcgat gccatattcg ctcaacagct caacggaaac ccggcgtgga atcagtttgt
    41821 acaaggcgtc ggcaacttcg gccggcgtca tggctccttt tggcatactc atcctcccga
    41881 aaggggtccc agcatactgg agccccgcgc gcattgacaa ccctggacgg tgaactgcca
    41941 cgcggcgtcg attgcttgtc cgccggaaag gcctatgcta catagagcct catcaccgtg
    42001 agatggagca ccatgacacc tatgcgacct gttcgagcgc tgttcatcgg cctcgccttc
    42061 ctcggcatcc tctgggccgc accgaccaca gcagcagagg tgcaggagcc acgctggggc
    42121 ttcagcaccg atcttggcct ctggagcgga accaccaacg acaccacctt cgcactcggc
    42181 ttcgggctcg attactacat ggaccagaat ttttcgttcg gcgggatggc gctcttcacg
    42241 ccggtcgggg acctgacaca gatcggcatc gcgggagtcg cgaagtatca tcttcgcctg
    42301 aatagcggat tcaatctggt gccgttcgcc ggactaggca ttctccacgc ggacctcaat
    42361 cgaggcagcg gcccgaccaa agtcgatcgc aacgacacga gccatttcat tcccctgggc
    42421 atgtcgctgg aatatcaggt cgggccaaaa atcgccttct cgaccaccct catggtaaac
    42481 ctgcaccaca tcaccctctc gccacccgtc ccgaacgaca acaccagcgt ggcactcctc
    42541 ttcggtatcc gctgggggcc gtaacgaggc cacagtgaac gacagctccg gcgaactgca
    42601 ctgagcatgt cgaaaatttg agtaccgcga gaacgcagcc gcacgccctt cggtttcaga
    42661 ttccacagcc gctcaaacgg gtttccagcg aagccgcagc aagtgaaggc ccgaggcgta
    42721 cttgagcgcg gtaccttgag ggtctgagcg aagcgagaac gacgccacaa gcacatcaag
    42781 cacttcagtt tcagactcca cagccgctca aacgggtttc cagcgaggcc gcagcgaagc
    42841 gaggaggcga ggcgtaccct tgtggtacgt tgagtcccgg agcgagacga gaacgaagct
    42901 ggggaaccgt ttcagcggct gtcaggcggc ttgggcgggg cgccaccgca accccacatg
    42961 caacaattcc tgcagcaggt ctttgtaggc gaggtcagct ttttcggccg agtcggcaaa
    43021 atcttcgccg gtggcgatct gaggattggg attcgcttcg agcacgtaga cgttaccgtc
    43081 tttgtccatc cgcatatcga tgcgagcgta accactcagc cccaatgcac gatagacgcg
    43141 cttggccagg tgctgaatat ggtcgacgac tccgtccggg aggttcctcg cttcgcacga
    43201 gcagatgccg tacttgtcct ggtacttgcg gctccacttc actcgctccg tcgcgatgcg
    43261 gcgggcctca tccggcatct tgtccatcac gagttcccag accggaaaca cttgcaggtg
    43321 cgcattgccc atcaccccga catagagctc gcgtccttcg atgtaccgct cgatgagggc
    43381 tccagtgccg acattgtcgt ggataaatgc cacccgttcc cgcaacttct catcgtcgtc
    43441 gacaatcgag gcctgcgaaa tccccagcga tgcctcttcg ctgatcgact tcacgatcaa
    43501 tgggaagggc aggtatttgg gtcgccgcac catgcgatgc aggggcacga ccatgaattc
    43561 agggtatgga atacgatggt acgacatcac ctgcttcgcc agcgccttgt cgcgcgcaag
    43621 catgaggccg cgtggattgc agccggtgta gggaatgcgc atcaattcga gataggacac
    43681 cacgttctgg tcgtagaccg cccgcccgtc gaactcctcc agtaaattga acgcaatgtg
    43741 gggcttccaa tcctcgacgg ccgcacgaat cacttccaga tcgcttttga ccccgagcgg
    43801 ctggacatca tggccgagtt tccggagggt ggagaccaca tcgtactcag tcttccaggc
    43861 cgcgccttca aggtcctgcc cgttcagttg atcgggaggc acaagatcct tgtgcatcaa
    43921 cacaagcacg cgcaatcgtc tcatagtgcc accttgtgcc ggccgctgtg cagatagttc
    43981 atggtctgca ccgtcaggag aattgcaaaa tccagcttgg cctgctcttt gtgctgggtc
    44041 aggtgtaatt tcgacgcctg acatcgttcg atcatccctt ccaacacctg atcgatggtg
    44101 tactgatatt cgccggtcca ttcagcgacc cggcgacgga tttcttttct ggtccgacgg
    44161 agaaactcgg cggcgctggg attttttgca taggccggcc cgtcggaaaa cagcttcttc
    44221 agatccgtat cgtacgacgg cgcgcgcccg atgccgtaat gctttcgctt ctcttcgtaa
    44281 tgatcgcgca gcgtcttata gatgcgaggc agcggatcca gcagctgtcg cccggtgacg
    44341 accggtgtcg tgtcggccaa ttcgcccatg agccggtcca tgaaatccag cttcttcatc
    44401 accggccagc ctcgataccg ttccttccag agcgagtgcg gatcgagcca cacggcaaag
    44461 gtttccgcga agtcttcatc cggatggctc tgggcatacc agacatccag gtggcgcacg
    44521 aaactccggc tataggggcg cggcgtgtaa tactcggggt agggctggga cgaacgtccg
    44581 aagagtttct ggcgacgacg acgacgacga agcaggtagg cgttttcaat ggcatggccg
    44641 gcctcgtgcc ggaggatccg catgcaccag tccctggtgc cgccttccac ttccaacatc
    44701 tgactcgctt ccagttttgc cagccgggga tgcgcaagat aaaacggcac ggcaattccg
    44761 ggcacaccat ccggcgtgaa ccattcgtcc gagatccaga agtgtggccg gaaggtgagc
    44821 ctgcgcgcct ccaactcacg agcgagttgt gcaatgggct cttgatagaa ggtcccttcg
    44881 aggcgcaggt ccagatcgca catgcgcaga tcgaggagtt gttcgtcggt ccactccgcc
    44941 cagaccggaa caggatcggt cgcgccgatc ccttgtccgc gatgattctt tgtccgcccc
    45001 atcgttcagc caccaaaaag ccccgttcgg cgccgttttg ttctgtttca actatagcag
    45061 ctcatggaaa ttatgcaggg cggtccccct cgaccaacga tgcaccactc agggaaaacc
    45121 cgcggacggt gagcgatgcg aaccggccac ttctgtttgg actacagaag cggtcggtta
    45181 cggattacag ggcgggctca cgattgagca gatggggaac tccgccccag atgtggtcga
    45241 tgacaaactg cagatagtca cgcgattgat ccggttgttc ccgccagtcg gggatgatct
    45301 catgcacgtc cagaatcggc tgatcgattc cgtaacatac gtgattgaag agagggactt
    45361 ctcgaccgaa ttccccgcga atccacgcct gatggttcct gcccatggca atgacgacgg
    45421 cggtctcctc gatcaagtcg cgcgtcagct tccgttgacg gtggcgggac ggatcgaccc
    45481 ccttctcacg aaggccggcc aggaccacgg gatggatctc ctgcggtaac gcctcgatgc
    45541 cggcggatcc gatcaggtac ccgacccgcg agccgagctg agccttcaac gcatattcgg
    45601 ccacgaggct tctgaaaata ttgcccgaac acacgaacag aatcgacgtc atcgcctcag
    45661 cctgtcagtc tgaatgcccc tgttcacggc tatcccaatc acggccccgg ttgcgctaca
    45721 atgccgggcc atgcagagca caaccacccc accatcccca ggcaccgcac agccgaccag
    45781 gcactccaac gagatcgaac gcgtgcacac ccgcctctgc cgcctggtgg gacaagccat
    45841 tgccgactac cgcatgatcg aggaaggcga caaggtcatg gtctgccttt ccggcgggaa
    45901 agacagttac gggctcctgg agatcctgct gtcgatccgc agccgtgcgc cgatccattt
    45961 cgacatcatt gccgtcaacc tggatcagaa gcaaccgggg ttccccgccc atgtcctccc
    46021 ggaatacctg accaggcgcg gcgtgccctt ccatatcgaa acgcgtgata cctactccgt
    46081 cgtcaaacgt ctcatccccg aaggccagac cacctgctca ctctgctcgc gcctccggcg
    46141 cggccatctc taccggttgg ccacggaact gggcgccacc aagattgccc tcggccatca
    46201 ccgcaatgac attctggaaa cgctgctgct gaatctgttg ttcaccggca agatgaagac
    46261 tatgccgccc aagctgcgtt cgaaaagcgg ccgccatgta gtcattcgcc ccctggccta
    46321 tgtgaaggaa gccgatctgg cgcggtacgc cgccttgctc cagtttccga tcatcccctg
    46381 cgacctctgc ggctcacagg aggacctcaa gcggaagcaa gtcaagacct tactgcagga
    46441 ctgggatcaa cggttccccg gttccaacga cagcatgttc gccgccctcg gcaatatcgc
    46501 cccgtcgctg ctgttggatc gaaccctatt cgacttctca tccctcaagg cagatgcctc
    46561 cccgagagaa ccagccgcga gtgattctga agacgatttg ctctagccca cgcgctgctg
    46621 cctatagcag gctgtgggaa aacccggttg gcatgcgaac actcagatgg tccacatcga
    46681 gtggcctagc agaagaatac cccgcaagat gcgcaaaatg gccgtccagc gttgagcctc
    46741 taagcgatgc gagaacgccg ttggcggaat ttttccgcat cctgctagac agaccggcgg
    46801 atgcgaaaca tctctagaag ggattgccgg agcagtaccg gaagtcagac acgctggcgg
    46861 agatgggtgc gacggccgca tcgcaaacat cgataaggat agtagccgat caggaagaag
    46921 aaaaaatcca acaggccctg gcggcgggaa cgtttcgtat aacctgaaca tttgccgcaa
    46981 tacgacatcg tacactctca cttgttgaac atccggtcca ctgcttccaa cggccgggat
    47041 tggaaggaaa tatcctactg aaacatccta gggccatgca agtctagttt gcacgacgtg
    47101 tccgtcgtca ctcggccacg ggagtcgtct caacgtgaca caattgctgt cggtcgatca
    47161 ggtgcgcaga cgtgccaggc aaggaagtgt gccggactca gaccggctag gttcgacggc
    47221 ggagacgaaa ccggtagacg caaatggtgc agcgaaatgg atagaacccg atgagatgca
    47281 tgaaaaaatc ccgccaacca tgacgggtca ctcgcttagc ctcacctgaa cacattggac
    47341 aatagaccat ggactcttgc ggcgcgcctt tctccgtcgc atcgggtaag atgaccgagg
    47401 tgcgcgggga cgtgagtgtg aatcagtttg ctacggggac gcaagtccca tcgaaggagg
    47461 ccggccggcg acatgaagcg gtccacacca tcccgccggc ctgcagccga gcccccgctg
    47521 tggtcgatcg cggccgcagc cggcgtgatc atcctcaccc cgatgttgct gtattccctt
    47581 gcgcctgaag ggccgattcg cgaaggtgat accgtcttct ccagcggagc ccacaaggta
    47641 tcgctctttg aacctgaccg ctaccggcag gccggctacg aggcgacctg catgctcgat
    47701 ccgaaagacc cgctcattgt gacccattcc ccctcagaag atgagtcggg cgaggtgatc
    47761 atcgcccagg tccaaggcaa aagcaccatc gagtggccgt tttgcccgcc gcaagcggaa
    47821 cttctcgtca aacgacacca catcacccaa cagcccagca tccttcagga catacgtgac
    47881 ggactgctgc gcctgttcaa accttcgtag cgacagaacc ctccccgccc ggcgttacgc
    47941 cttgagcaac gacgcatagt gctgacgaaa cttggccact ttgggtccga cgacgtaggc
    48001 gcagtagccc tcaaacggat tccgtgcaaa atactcctgg tgataggctt cggcctcata
    48061 ccaccgggtc gccggcacca cttcggtcac aatcggattg ggatagacgc gctcccgcgt
    48121 gacggcagca atcacctcct gagcaatctg ctgctgctcc ggggaatggt aaaaaattcc
    48181 ggatcgatac tgcgtgccga tatcgttgcc ctgccgattg cgcgtcgtcg gatcatgaat
    48241 cacgaaaaat acgttcaaga gatcacgata cgtcaccaac cgcggatcga acgtgatccg
    48301 cacggcctcc gcatgccccg tgtgcccacc acacacctgc tcatatgtgg gggcatccac
    48361 ctgcccgccg atatatcccg actcgaccga ccgcaccccg ttcacctggt cgtatacggc
    48421 ttcaagacac cagaaacagc cccctgccaa tgtggcgatg tccgtcgttt cctgccccat
    48481 gtcccacctc gtcgttgact cgcaccggca ggctaggcct gccctccatt ccaccactcc
    48541 cgcccttcac gcttcaacaa agcctctgcc tccggtggcc cccagcttcc actcgggtaa
    48601 ttcgggaacg acgccggtcc gggaagggac ttccacacgt cgagaatcgt ttgcacgaca
    48661 gcccagccca gttcgatatt gtccgcgcgc tggaagagcg tcgcatcccc ggccatggca
    48721 tcgtgcaaga gggtctcata accggtctgg ggggcgttgc cgaaatagtc ggcatactga
    48781 aagtccatgt ccaccgtgcc gatccggacc gtcggtcccg gcaccttggc atcgaaacgc
    48841 agggaaatgc cttcatccgg ctggatgcgg atcacgagca cgttcggaac caatcgatcc
    48901 acctgagttt ttctaaacaa catgaacggc gcccgcttaa actgcaccac aatctccgtc
    48961 aaccgcctgg tcatccgctt cccggtccgg agataaaacg ggaccccggc ccagcgccag
    49021 ttatcgatga acagcttcat cgccacatag gtctcggtca tcgactcgga ccccacatgc
    49081 ggctccgacc gataccccgt taccgccttc ccgtcggacg tgcccgggcc atactgcccg
    49141 aacgaaacaa accgcagcac gtccagcgaa ctcagcggga caaccccgcg caacaccttg
    49201 gccttttcgt cgcgtacggc atcggcggca aaggagttgg gcggttccat cgccagaaag
    49261 caaagcagtt gcagcaggtg attcggcacc atatcccgca acgcgccggc ctgttcataa
    49321 tagcggcccc gatgctccac cccgagactc tccgccacgg tgatttgcac atggtcgatg
    49381 tactggcgat tccagaccgg ctcgaagatc ccgttggcaa accggaagac cagaatattc
    49441 tgcaccgtct ctttcccgag atagtgatcg atccggtaga tctgccgttc ttgcaagacc
    49501 cgttggagct cgtggttcag cgcccgcgcc gattccaggt catgtccgaa cggtttttcg
    49561 atgacgacgc gacgccatgt gccctcacgt tcgaccgtca gaccatactc tcccagatgc
    49621 gtcacgattt gtccgaacag accggggatg gtggccatgt aaaaaatata gttgccctgg
    49681 gtacccgagg acgcatcgac ctgctgcagc aactcgttca gccgtcgata ggtggcgccc
    49741 tcttggaaat ccccggccag gtaatgcacg cgctccgaca acgcctgcca gaccgatcga
    49801 tccacggtcg ccggcagata gtcgcccatc acccgatcca acttctcgcg aaactccgcc
    49861 gttgtcatct cgggacggtc caaccctacg atggcgaacc cctccggcag gaaatggccg
    49921 gccatgagat tgtagaccgc cggaatcagt tttcgcttcg ccaggtcacc atcgatcccg
    49981 aagatgacca tgacgcaagg agtgcgcagc tgggggaggt ccatcagagg ttccgcactc
    50041 atcgctctgg ccgttcaatc aacatggggg catcctgcct gatccctgct acaaccgcaa
    50101 cctcctcagt gggctcatga cggttctgcc atttgagtag gcgcccggcc tgccccgtga
    50161 tactcatgga agggacgaca cacccgacaa ccggacaggc acctccaaca tgccgctgcc
    50221 atctcctccg tcacctgctc ctcctctccg agtcccgctc cctggattga ggcggtggag
    50281 taccttcgcc gcactctgcg gcatgctcgc catgatggct ggcgccggat tcgtgagcgg
    50341 cacgacgcct cagctcactc cccgggacgt gcacatcgaa gtgccggccg atagtctgtt
    50401 cggctccctg ccggtcaacc ggggtctcac ggtttcaatt catgtcccgg aaccaccggt
    50461 cagtcgcgga gaaccactcg tcgccgtttt tgaaaccccg tcctccgcac cctacaccgt
    50521 cccgttggaa atcgatcgct cccggcatca ctattccgcc accgtcgacc tcggtcggct
    50581 gtcaggcacc atgggcactc ccccgaaagc gaccgccctt caggtgctca tcggccgccg
    50641 acaggggtta cacgtggaag ctctcgtgcg ccgtaccgtc gtcgtcaccc ttgcgattcc
    50701 gggatacgcc gatcaccggt cagcccggac ggatttcgcc aaccagatgg ccaatggttc
    50761 agggcctccc cgaaaccaac cagccgacct ggcgttgctg gacgggcgag ttgaagagga
    50821 ggaattagtc gggaacgggg gactgccaca gcaggaaggc tattggaaga tgctgcaggg
    50881 actgatccat aaagggatgc cggatggagc ggccactcgg cgcgggagag agatcggcag
    50941 gatgccgggc atcgggtttc ggctgtatgc caacggcgag gctcagttga tcgaggtcga
    51001 acgtagttca ggggatttgg aactcgatca ggcggcggtc ctggccgtcg tcaacgccca
    51061 cccctttcct ccgtttccgc ccggaaccag cgacacccac gtcgatgtgc atgtcgagat
    51121 tcccggcctt ccccgttaga acggcaccgg gtctcaccgg accccggacg cgctaattgt
    51181 tgtgcgtccg cttcagccag tcgataaagc gctggagcct cgtctgctcc tcgtcgtgca
    51241 cgtgcacaaa caccagtcca aagcctgtgg cattcgtcca acgcacctcg gcgacctcga
    51301 ccgtcatggg ctcatacgga gccggaaact cgacgtggag ggagagacag gcgcgtgcag
    51361 ggagcgactc ctcgctcacg acatggcaac cttcattgga cagacttgtg accaacccgc
    51421 ttccggcaat ggcattaccc gaaaacgtga gcggcacttc gatttcatag cggggactat
    51481 cgcgacgctc cacatgtgcc tttcaaaaga tcagggatcc ggactcgatc cataaaaatg
    51541 tccggaaatg gatacgctgt tccgctcccg aaatcaaccg tgctgtccag ggtatcacga
    51601 gcctgcgcac cggccctgtt gccgctcgag gcctggacga tcagtcggtc ggcgtatcgc
    51661 ttaggataca aggccgtatc tcttgtgaca gcctgaatca ccccgcccat cgttgaaaca
    51721 accagacacc cttgaaccac cggaatatcc agtccggcat gccacccact ccctgaaagc
    51781 cggttcctgt ttcggctggc acgagggttg ctcgctacaa cagtaccagt agctcgctcg
    51841 acctgaattg gagcctcgaa gggagcgtct catggagcac ctatcggaga ttgtgattac
    51901 tgtcgtggcc ttcgccgcct tgatcgggca gtggatgaca gacctccaca ccatgccgaa
    51961 caggaaccgc cccacaagcc ccgaagagca atgagactgc ctccgtcacc atgcaaggac
    52021 tctgaggtca ggacgataaa ccatgcgctt cgccaccccg atcgcgcccc ctcgaagacc
    52081 caccggtagg attctggccg tcatcgccct tgtcctcatc ccgttccctg tgagcatccc
    52141 cctgtcgctg tggctgacag gccggcgcca tttccccgac agtcctgacc tgccaccccc
    52201 tccccacgag cagatatcct aagggtttca ctggatcaac ggattgtggg gggatatccc
    52261 ggagcagtcc cgctaaagtt caggaaaaac cctgccgaaa aggtccatac gccctcagga
    52321 acgagggcca aggcaaggag gccttatcat ggacatggat cttcgttcat ccactcgggt
    52381 cgcagtgacc taccctgttc gcctgtcggg cgactcgatg atcggccagg gaaccctcat
    52441 caacctctcc ggacccggct gcgcggttga aaccacgctg cccgtccaac cgggcgacta
    52501 cctcgaactc catgtgatgg cgccggacca ggcgcgaccg ctcacggtgg gcctcgcaaa
    52561 agtgcgctgg gccaccgaga aaaaagccgg aatcgaattt atccgggtgc gtcgggatga
    52621 gcagagccgc ctgcaacgcc tgatcggcca ggtgctcgaa gagtcgacca tggaagccaa
    52681 cacgaacggg cgcgaactga ccgctgccta actagaagac atcggcgcga ctcagcgaag
    52741 agcgcgatag ggtaagacgt cgggagatgc ggggggtaga gttcagtgcc ctgccgtatc
    52801 cttggcggaa tcggccatcc gcttgacgat aattttccgg cccacggtca tgagggtgaa
    52861 ggacgtcgca aacgcgatcg gaacgaagat cgccgtcgcc acattcgcat tcttcagcca
    52921 gcccatctcg taacaggcct tcagcacgta acttcccaag cccgtcaggt agtaggtaat
    52981 cacgatcacg gacagactct cgaccgtgcg ctgcaggatt gcctgggccc gcgtggtgga
    53041 gtccacactg atcagcaact tcatgttttg atcctgcaat tggatattct gctcctgcag
    53101 ccgcagttcg atatgcgtcc gaagcacggc caccgtcgct tcaaaatcct tctccatggc
    53161 atcgatacgc cggagcaact gctggtagcc ctcggtcacg ccggtgattt tccagctcac
    53221 atactctgag atctgccgga gcggtggata gggtcgctcc tgcaacgccg cgaggttgct
    53281 ctgcacgata cggtcgtacg gcaccgaggc ggagagcctg taccgcatcg actccgccag
    53341 ccggctcacc tgcaagaggt cctgggtcag gaccgtcagc catttctgca tggtcggcgg
    53401 ggtcgttccg ccgagctgct cgatcaagac ggcgcgctga tagagatgtc gctgctcgta
    53461 gtcatggatt tgatcgacgg cgcgtgaaaa cgccttcatc ggcaacatca cgaggtggta
    53521 atagttctcg atggcaacga tcgtatccac gattttggcg acctgttgcc gaagcgcctg
    53581 ttcggaggtc gaacagatca gataccgttc ccggtcgaac tcgtccggcg tgaaactcgt
    53641 gaccgccacg atgtcgtcgc ccaggatacg gctgccataa accatggcgc cgggcagccg
    53701 agccggaagt tcctgctcca gcggcagctc ttgcggcaaa aataacaggt cgagcgcatt
    53761 cacgcggatg ccgagcgggc ttagcggcat catgtagccg ggaaacgtga tcggcccgaa
    53821 atcgagcggg gtggtcgcgt cacggacgac atgccacacc tggtaactgt aatactccgt
    53881 gtgggcttcc cacacaacga ccagacggtc accgttggcc gcgactttaa acccgtagcc
    53941 gaaccggtcc tcgatggcgc cttcggaaat ctccagggcc tgcaggagct gctggaactc
    54001 ccgacggcta ttggggcgtt caaccggcgg attggccata cgatacgcca catggtgaat
    54061 atgggccgga acaccgagcc actgcgggag atactgttta ttggattgat ggatccggtt
    54121 caaaaatccc ggcgggcggg aatctgttcc ggaaggctcc acgtgatccg tcataacacc
    54181 cggcgctccg aataaaacca gaggaaggcg cgtcttaaat ccaaccccag caggcctgct
    54241 tcagaatgaa gcgggtaccg cagccatgcc gctgcggcag cagaaacgtc cctcgtcact
    54301 actcatcttc ctcttcaatg tcttcgatgc cttcgtagct cacatcccct tcatgaatgg
    54361 atgtcgccat cttttcacag gccgactcaa tgatctcttc agcctcttcg ggcgaatcgg
    54421 ctgaaatcaa gaatttcacg gtaattccaa agcgctggtt cacaatcgta ctccttcgac
    54481 gaattagggg gcagaaagtc tgtcacctgc tgtggctcaa taccataagc tcccgcgtat
    54541 tgtcaccctt ctgtctcacc caaagtaaac agaacccggg cgaatgggac gacccaccac
    54601 ccggaaacgg gtccgacggc tctcgacggg gacgaccgca acgggcttac aggtgtgaga
    54661 aaccgatccg gcctcgatgg agaaagatgc ggaatcggga gcagaaaggg aaggtcagtg
    54721 agagatgcgc ggtgtctaac ccggattatt catgaacccc gttgcgaggc gtttgagacc
    54781 gacgcccgcc ggggcttctc gcacgtaagg ccgacgcagg cgtacctgca gtccgtcgag
    54841 gaggccgaac gagaatagcc ccgccgggcg aaaggatcgg acgacggagc aggtattcat
    54901 gaatagtccg ggctaagaaa cctggtcccg acgcccactc acgggaaggc gccgggaccg
    54961 ctcgattatt tacgcagcag catgtggccg cgccgattct gttgatggca ggcctcgtcg
    55021 tggtcggcgc agaaggggcg atccttgcca tacgagacaa tcgccacctg cttcgcgttg
    55081 acgcccagat cgaccaggaa actcttggcc gccttggctc gtttttcgcc gagcaccagg
    55141 ttgtaatcgt gggtcccgcg ctcatcgcag tgcccggaga tcttgaggag cgccttggga
    55201 ttctccttga gccaatccgc attggtggtc agcgacacca cgccgtcgtt gtccgacacg
    55261 ctgtatcggt catagccgta gaatacgtcc ttcagcccgg cttccatggc cgcggcttct
    55321 tctcggcgaa tttctgccgc ccacttctcg tcccgttcat tcgacgtcaa catactgccg
    55381 atcggactcc ggcttaatcg ttcttctcca gcaatctggc cattcaccag cggggaaaag
    55441 ccgcgcaggc ggccgccttc cgccgctgtc atgtcctgtt cccgcccaag cgagggatac
    55501 tgtccgtcac gccccgactc cccgcgtccg taccccccag acgcgtcggc agaggcgccg
    55561 gacgaggccg acaacgaaga cccagaattg gaggcttcat ccgagccgga ctggaaccat
    55621 ttcatattgc tgcagcccgg tgccgttaac aaaaccattg ccattccgcc caacatcagc
    55681 tgcgacgcgc ctcggtactt catgaacctt gctccttgca gggggtgtaa cctgtgctgt
    55741 acgtaacaaa tatagcatcc ggcttaccgg ccccagcctc tcagcgatga gagttgtgtc
    55801 atccaatatt ttcaattggt tatgacaatc acccgtcaga gccctggcga acaaagtccc
    55861 tcagaaccac aaataaaagg cttttacgag gaatcctctc atgttcgcga ggaacaaagg
    55921 gggcaggtcg aatagcaggt ctgggtcagg atagaggtgc tctgtgatcg cagacactaa
    55981 tgatgtgcaa gcgagccggc ggatgggttt ggaagggcca cgctaccgtg aagggatacc
    56041 tcatctcagg acaatcgatt agattcgatc tcagccatcc aattaccaat ctcggacatt
    56101 gttctcccct cgcctgaaag agccgtgaca tcggtgatga ttgcaattcc tgcatcgcaa
    56161 aatgatgttc tgtcgctggc ataatcccat gtccatggcc aagtggtatt gataaattta
    56221 gccacctcat acaactcgtt cgacccggta tagcctgcag cgacacatgc ccgggtgatc
    56281 gcacagccgg tgggaagttt gttgtgatcg ggcttgctcc acgcctcatc gacgtccggc
    56341 agagtgaatg cccccatctt taaggcctct gacaattttg gagttgactg tctaccaagc
    56401 cttcgtctcg gcgcatcgct ggaagtagcc ccacccttct tctggcccgc atgctctttg
    56461 attctaacct ggatcgaccg atagcccatg tgcgtccccc tcctaacgaa atagcgaggc
    56521 cgtctgagtg ctcgaccaga gcggctcagc cgtcgattta taaggggagt acatcacacg
    56581 gaacggattt ttctaacgag agaatcccct ggttgtaaat ggtaaaaaat gaattgaagc
    56641 tgtagtcctt gaagcgacgg gaccatacaa aatatgaggt agtcaattga gcagcgacca
    56701 ggagcctgca gcaaggggaa taaaactcgg gcagggaatc tcacagccat gaagtcatca
    56761 gtggagacag gcgggatgaa gtcagttacg tcaggatact gcgcgacgaa gagccatgcg
    56821 tcgcatcagg cagccacctg cccccggcca atggcgggcc ctcatgtcgc aaaggaccgt
    56881 cgagcatgca aggggctgca aacgaacggc gggcgaggat gaacttgcaa agcaacgatt
    56941 gtggtgcgcc cggagggact tgaaccccca accctcggat ccgaaggcgc acaaagcact
    57001 ttagagacag acagcgatca gggaggacag atggacatgt tctctttata gatcaatgct
    57061 ttttgcatca gtccatgagg atcaatgacg actgaggcgt acctgtgatc gtgcctaaaa
    57121 ccgtgaccga accgtgacca aaaccgtgac tagatttcct aacaaatgag cgacgacctg
    57181 gcacattcac tcgaacagat cagcgtggga gccggtacgt tccaagtaga ggaattcatc
    57241 atccgttcga taaatcagca gccagtctgg ggcaatgtga cattcgcggt gatgcttcca
    57301 attcccagaa agggcatgat ctctgtttct gacaggcaga actttttgga attgtaaaag
    57361 gtcaatgatg tcctcgagct catcgatgtc ctgtcctcgt tttttggcta acttcaggtc
    57421 tttgagaaaa cggctggtgg gtttgatgat gagcaaggct acacgcctgt ctggtttctg
    57481 agggcgcgga aggttttaaa ggaagggagt ttctccggtt tgttggtttc gtcaaaggcg
    57541 gcaatggtcg tggcatttgg aatggtgagg gggaagggaa taccttgatg gagttcaacc
    57601 tgagcataaa aaagccgtat ggcttctgcc tcactaatcc ccaggcgatc gaagattttc
    57661 acggctctct ttttaaggcc catatcgata cgggcattaa tacggtctga catggacaga
    57721 tgcgatgttt tcctcatgtg tgacattgta cgccatttgt cgcacaagtc aatcatctgt
    57781 cagaacaatc gccccttctc caagaaacga gaccattggc caagacgacg taaaaggcac
    57841 cctagagact gatagcgatc agggaggaca gatggatatg ttctctttat agatcaatgc
    57901 ttttcgcacc agtccctgag gataaaggac gactgaggca tacctgtaat cgtggccaaa
    57961 accgtgacca gatttcctcg gtcacaaccc tgtccccctc tccccaattg tatgtcactc
    58021 tggggtccaa attacctgat ggtcccaaca agtgttctag cagaagtcgc attcacactt
    58081 tctccaacga tgtggataga ggaattatta agaactgaga tacaaccgga aatctcgcgg
    58141 aaattcattc tgcaatagca gcaataagtg acatgcgtgt cctgcaagca gtagcgggct
    58201 atatctgcat agagcccttc gtcccacatc ttggcaacct gatcgcctga accactcttt
    58261 gattccacag ctaacgctct cgctagcaca tcaagtttta tgaatgcccg tgggtcccaa
    58321 tttgcccaga cagccatagt gtcgaagacc ggagtagtcc gaaacctagt cagggcgata
    58381 tcgacagttg gccgtacatt tcgaattatt gagcgcttga gtatgaaggg gagatcaaac
    58441 cctagcccgt tgtgaactat cacttgaacg ggattgatac gcgccatctt actccagaaa
    58501 ttccgtagaa ttgcggtttc atcattgccg caccaagcta tagcttcgac tggagaaaaa
    58561 gaatcctcta caaccaagat acccatgcaa ataatccggg agaacgtacc atcaaagcga
    58621 gacttctcgt actccttggc aaactgggta gctatcagag tgggatcaat atgcatcgcc
    58681 tcttctgctg aggcgatgcc ttgaagatag gcccattcgc cttccgaggc ctgaagagtt
    58741 tcaatgtcca ggacgatctt cactatttgc cttcttgctg tctctggaat taatgcgttc
    58801 gaactctgct tgaaatctct gaccctgagc cgggttaatg tgtttaacga aagcaatctt
    58861 tccggccagg cttgctttat tgacctttat ctccgaactc atcacatctg agcattcgtt
    58921 actcgcacaa ctgtgcagca tcttccgcaa cgctctcttg tattcttttt ttacattcgg
    58981 cttcttattt acaacaagtc cggcagtaac ctgacgccct gtgctgggta aggcccgcgt
    59041 cttccgctcg ttaacagaaa atccctcttc ttgaatgatg cgtctcacga gattcttgaa
    59101 cttctttgcg cggggcgccc cagagacaaa ggcatcgtcg cccaaaatag ttagatcgaa
    59161 atgctgttga tcagcaaagc cttggattct tcgcgctggt ctactccaga tcagattcga
    59221 gataagaggg ctcgtcggag ccccttgggg caattggtta ttgtgggtac acaaatctac
    59281 aagtagccgc gatacggaat cggaaatacc cacttggcgg aaagcgtcat aaaccatgga
    59341 gggtcgaatg ttcggaaaaa atttttcaat atcagctgtt agcaacatgg gcttttcaac
    59401 gtggggaaga gccgcggtaa cctgaccacg ttttcggcgg tacccatgga tcgctttatg
    59461 aaacggtgca aagaggagca ctttcttatg gataagagac tgcacccgct tcagctctcc
    59521 gcttggtatt agataggtac gtacgccacc gtcactttta ggttcgtctt tttgacggta
    59581 gaaatgagaa gcggtccgag atagtcgaaa tagagttcct tgggagactt cgagataaaa
    59641 tgcgagcatt tcaaccgatc gcatctttga ctttatttca gcccatttca tttctaaatc
    59701 gctcgttctt ctcttgttcc ctgtaaacgc aatcgctcca tttcgacaag caaagagcga
    59761 agacttttcc atcgtccttc tttttctgaa gatactcctt ctggatcctg ccctgcttcc
    59821 caaaacaaga ggctgactgg gacatcaagc tcgtttgcta tttttcgcaa cacagctaaa
    59881 ctaggctcgc gcttacctgc ttcaaccaga gaaagataat gtggttgaat gcccacctta
    59941 tctgccaagt ccttctgtct cagcccgatg gctgcccgta ggatcttgat tatcggccct
    60001 atgccaagat cttcgtgttt gttcatatta tataagtgct ttttttagag taaaaaaact
    60061 aggagccaga gagtagtttg gataatttcg tagcaactaa taccgctctg cgggacagct
    60121 tccaaaacct tgcccttgat aaaggatcgg tcttgtccag ccgttgcata atccggcgaa
    60181 tactacgaag tttccccatc atactctcca gcttcccaga agctagcgct gaagaaactg
    60241 caagctcctc aagtaccttt atggtctcgt cgaggaactt ctccgttcgg gtgtacttac
    60301 tcaaagtaat cacccccttt ctttccatcc gctgcgacaa atgcattcgc tagcgttgac
    60361 gcagtctatg cgaactacgc caaggaacag tcagtttcaa ccccaacctc aaatcgagat
    60421 agggacccat acacccaacg ctatggctag gtgtaattgg gacctgctga gttgtggcgc
    60481 gcattcgcca gcgctctgag ggaaaccctc agtttgcaac tggctacatg taggatgagt
    60541 gtgggagctg ttgagattat gctcagcgcc gttacccact gctacccttg ccccctctgc
    60601 agattgaaca ggacttgctg ttcccttaat caagtcctgt tcggcacgga tggatatgtc
    60661 gccccgaacg gagctttgtc aaagaacatg gggacagtcg cccccaatgc ttcctgaatg
    60721 tacctgaact agatctaact gtcaactatc ttacactatc agtgacacta tttatttctc
    60781 gtcaactatc tgaatggata ttctgcccca tcgaaccgtt tctttcgaat caaatcctcg
    60841 accatcaggc aagccatttg gctcgaggca gctacagaaa taaaagatcc tactttgacc
    60901 atatagatca agtgaagcgg gggctgcgcc cccgatcccc ccggccgtcc ggtccagtgg
    60961 tccggaacgg ccgggcggtc tcgttgagcg gagtctttgc gtgaccaatc tccccatggt
    61021 ttcagtattc ctcttcgctg tgtttgttta agcaggaata gccccgaatg gcggccaagc
    61081 gggacgcatc gccgccttac ataaggacgg agcatcattc cctctcaccc gtgacggata
    61141 gttgacgggt gacagggaga aattgattct aaggtttcac cgtctcccag tgagccccgc
    61201 acactccaca cagagccaga tttcgcacgt cacatccttc tccggataac agagtcgcac
    61261 ccaggctcgt tcgaattggc agaattcgca cagccgttca ttcgtatgct cgggacagag
    61321 ccaggctttc cgtgcccggt cgtaggtagt cgcctctcgt ccacaacaag ctggaccaaa
    61381 gaggctcggc agcttcagat cgcatcgatg ccagatcatc ctcacacctc ctgatagacc
    61441 agccccttcc ccagcacgag ttgcagaatc gtgccgctaa accaaatggg atgccccgag
    61501 agcagatcct cccaggtctt gatgaacacg atccgaggga tttcgatctc gcctctgatc
    61561 cgtcgatagg cgttcctggt agaccattgg tgccgcatct cttcccacag tcgactgatg
    61621 ggaaacccaa gggagcgttt ccaggaccaa cacatattca cgtagccgca ttgatcctgt
    61681 acgtattggc tgatgacata gcggctcaga cggttccggg aacgatggct gggctgatag
    61741 gccttgatcc atacgaccgg cgccccatgg agggcttgcc attgcgacga gagccattcc
    61801 tgggagatcc agaagcgccg ggcgcgttct ccatcgggca cccgccaggc ccaaaagatg
    61861 tggagcacgc catgcccttc ttccgtccgg acctggtaat actccaagcc ctgaaaccct
    61921 agctggcgct cgatccgttg gcgcagctgc ttgtggtgat aggtcagctt ctccgcatcc
    61981 ccgccttctg ccgtggagag cgtgacccag aggacttgga actggtggca ctcccagaac
    62041 cagagcaacg acctgacccg gtggtagccc cgtttctgct ttctggtcca ttcaccggcg
    62101 accggctccc gccggaactc cttccagcgg ctcatcggac accttccgcc tggggaatcg
    62161 ccggcctcag gccggcagcc agaatccgct ccagttcctc aatgggaatc cgcacggctc
    62221 gcaccgacgg cttcacatag gcaatctgcc gtttcaggat gtatttcctg atcgtgcttt
    62281 ccttcagtcc taaccgttcc gccgcttctc gaatggtgat caattttgta ctcgggacca
    62341 tcgggcacct ccttggaaaa atggttgcga agagatcacg gctcatattt tttaggcgtg
    62401 attgctcttg acagccatta gcagtgagga ggtacacttg ccaaacaaat tcaggtggag
    62461 acggcccttc agcacgacac ttttttcagt ggtgttggga tcaggaagac ggagagggtt
    62521 cggcatgaaa aaggagcaga aggcatgagc gcacagggga aagccgcgga ggaattccaa
    62581 caggagtatc agaaagcgat tgagcgcatc cggacgatgt cggacggcgc cgtcgggtgg
    62641 gtactcaagt tcctgcaaat ggacctagag gccctgacac cgaccgagtg gacgctggtg
    62701 gcgtttgaag tcgcggcctt tgtcgatgag acgggggacc gatacggcgg gatggtggcg
    62761 cctgaaagtg gctggagcgt ggaaggagtg ccgcatgcga agaactatca gacgattccc
    62821 agtcgcaaag aagcccagga catgcaggcc acagtcctgg agcacttaga gctctattgg
    62881 catgaaggat acaccgcctt cacctttccg cagatgacac tggtcgtagt atcgccgggc
    62941 agcttctccg acgagaccgg gacaatcttc gtgagcgcga aacgcaaggc gaaggagttt
    63001 gagtaccgct ttgtacacct cttggcccag tcgggggact acattcggcg ctgcccggaa
    63061 tgcgacaaga tctatttagc gatcagacgg gaccaggtat actgccaccc gcgctgccag
    63121 aaccgagtcg cggcgagaaa gtggcgcgag gcccagaaga ccggggagcg aaaggagagc
    63181 cgccgtggca agaaaagcgg gaaaggatag aggggttact cagcgcaaag gccgcgaagg
    63241 ctggtgggtg cgtctgtatg ccaacggacg ggagcgctgg catcggtgtg ataccaaatc
    63301 acaggcaaaa gcgctctatg gtcgattgaa agccgatatt cgggaaggga cctacttccc
    63361 tgagaaattc gcccctcgaa aagacatcac ccttcgggcc tggatcagtc gctacctcga
    63421 aggaactgtg aaccgtaaca aagctggcga agtgctctat ggacggcgct ggtccctgct
    63481 tattgggagt cgcctagtca cccagattac ggtcgacgat ctccggcgga ttcaggccaa
    63541 aatgcgcgcc aagatgaaag tgaataagaa ggaaccgcaa agactgtggg cggatgccac
    63601 gattaacaga cattttgcct tccttcggca tgtgctcatg ttagccgtta aggacgacaa
    63661 gctcaccaag aacccggtct ccagcctcaa gttcttccct gaagccaacc ggacgcgctt
    63721 cctcacgagt gaagaacttg atcggttgag aggcgtgatg accccggatg attggaaatt
    63781 ggtggccttt gccgtcgaga cggggttgag gcgaggagaa cagttctcgc tccgctggaa
    63841 ccaggtagat ctggagaacg aggtcctgac cctgcctatg cctaagggag gcaaaacccg
    63901 gcatgtaccc ttgagcgaag gagccaaaac cattttgaga tcctttgact cgttccttcg
    63961 gtcggcttgg gtgttcccag gcttaaagga cgtgactcac cctatggaca gccgggcctt
    64021 tcttcggcgt gcctttgagc ctggcctgag gcgagccgga atcaccgggg cctgttggca
    64081 ttcactccga cataccgcag ccagccgccg tgtcatggcg ggagtcgatc tggtgtcggt
    64141 gaaagagatt ctcgggcatc gcgacattga gacgacgctc cggtatgccc accttgcacc
    64201 aggacatctt cgggatgcag tgaatcgagg gagtctggct ggaaccgtga ccaaaaccgt
    64261 gaccaaaccg gagggggaag agcgagaaac gatgcaacct gttgattatc tggtgcgccc
    64321 ggagggactt gaacccccaa ccctcggatc cgaagtccga tgctctatcc aattgagcta
    64381 cgggcgcgcg ccggtataag aaccatgtta ccgaggggat gtcaagaaac gtggatcgca
    64441 acggtggcgt tcacaggcag agggcctcaa cgcctcagac agccgaccca cgagatatca
    64501 gctgcagaac gcgttcggca acctcggtgg tcgaggcccc atcggtcgat acgacgtggt
    64561 tggctgccgc ctgatacttg ggtgtgcgct cgttcaacac gtcccgtatt tcctccgtga
    64621 aggacttcgt gcccgtcaat gacgggcgct gggtatcacc accgatccga cgcgtgatcg
    64681 tctccacggt cgcggtgagc cagaccaggg tcccggttcg tcgcaacgcg tccacgtttt
    64741 ccggccgcag aatggcaccg ccgccggtat cgatgatcag gtggtcccgc gcagcaaaat
    64801 cacggcagac ggccgactcc aagtcgcgaa agtgatccca cccgaactgt ttgacgatct
    64861 caggcacgga cagctgtgcc cgcttcacga tttcggcatc ggtcgacacc acggtacgac
    64921 ccagcttgcg cgccagcacc tttccgaccg tgctcttgcc ggtcccacgg tatccgatca
    64981 acacgagatt catgcgagga tccatgccct gacacgagga tgttgtgcgg attcctgctg
    65041 cggaaggccg accgctgtct taaccaaagc gagactccag aacccgtcgc atgacatcgg
    65101 ccggtgccgg ctgcttcgtc cacagttcaa attgcagcac ggcctggttg aggaacatct
    65161 ccaacccgga gatggtccgg cagccggccg ccttcgcttc ttgcagcagc ttcgtttcgc
    65221 gcggattgta gacaatgtcc atcaccgtca gttcgggcct gaagagattg gtcggcacac
    65281 aggactcctc cacccgaggc gacatcccga ccggcgtgca gtgaatcaag gtgcgcacgt
    65341 gcggcaccca gttccgaagc atgtcgagcg tgagcggacc gtcctcaatc gggatgttcg
    65401 tcttctctcg caaatccttc accaatttct gccgctcaac ctcttcgatc ccgagaatcg
    65461 tcaacccggc gatgcccgtc ccggttgcca gcgcgaaggc aatcgctcgg gctgcgcccc
    65521 cggagcccag gatcaagacc cgatgcccgt cgagcaacgc cccgccttct ttcaacgcgc
    65581 gcaacgcccc ggacgcgtcc gtgttgtagc ccttgagctt gccctcttcc acgacgatgg
    65641 tattgatcgc gccgatatgc ttggcggtgg gctcgacttc atcgagaaac ggaatcgccg
    65701 ccactttgtg aggaatcgtc acgctgaatc ctcgcgcatt ccccaaggcc cgcacccctt
    65761 tcagcgcatc gccgagggct tccacccgaa acgccaggta aaccagatcc agccccatct
    65821 tctgaaaggc cgcattgtga atcgccggcg agagggaatg ttcgaccgga ttgccgatga
    65881 tgccgcacca ttgagtctga gtcgtgatgt ccatacgcaa gaaccttcta cttcttgagt
    65941 gcgacgttca gggccacata ctgagatcct tgcacctgat acagcaccgc cacatgatcg
    66001 tctttcttca gatccttcac ggccttcgcc ccaccgtcga aatgggtttt ttcattggca
    66061 acgaaggtaa accggcttcc tccgccgtct ttcttgacgg cgaattttcc ggatgtcgta
    66121 tccaccgaca cgatcgtgcc cacgaattcc gtcccacctt ccactcgcac cggctccacg
    66181 cttgattcca gcaatcctga caagacggca gccaagaaga gagccggaac cacaactgcg
    66241 ctcatacgtg acatgccact catgatcgcc tccttgcttc cggcaaccag gtgccacagc
    66301 actcttcccg agtcgttgcc ctccgctgca gcagagcggt cgccttcttt tgcttacccc
    66361 ttgctgatgg tcatgcctcc atcgataggg aacgtcgctc cggtcaccca ggtggcctcg
    66421 tcggatgcga ggtagagcgc catcttcgct acctcttccg gcttgccggg ccgacggatg
    66481 gcgtactgag ccatgatcgg ttccaggctg gcaggatcgg ccattaaagg cgccgccatg
    66541 ggcgtatcca ccagaccggg attcatgacg ttgcagcgga tgttgtcctg cgcgtaatcg
    66601 accgcgatgg accgtgtcaa cgcgtccaag ccgcctttgg acgcggcata ggccgacaga
    66661 ccgcggatcc cgaccaggct cgccacggtg gaaatgttga tgatcgaacc gcccccgccc
    66721 ttcaacagct caggaagaac tgcgcgggtc atccggaaga ctcccgtgag attgatgtcc
    66781 agcaccttcg cccaggtggc atcgtccatt tcgtggagcc gtttgccgaa atcgccgatt
    66841 ccggcgttgt tcaccagaat atgcagcttg ccgaaggtac gcagggtctg cgcgaccaca
    66901 tcctgcacgt gggcctcatc cgtgacggat ccgaccaccg ccagtgcccg ccctccgttc
    66961 acaccgatcc ccttcaccac ccggtcgagt tcttccttcc ggcgaccggt taccacgacg
    67021 gaggcgcctt cgtccgcgaa cagcttggcg atcgcctccc cgattcccgc attcccgccg
    67081 gtaatgatgg ccactttccc ctgtaaccga ttcatgcctc tgcgtcctcc ccctcgtgcg
    67141 cttcgtcatc ctgctcgccg gctgcctctg cctcgaccgt tttgctcgga cgcgaccacc
    67201 agccctcttc gacttttctg gcgacggtca ggaaacccga atgcgccacc atgcgatgat
    67261 cgggccggac actccgcccc tgaatcgacc aggtccgcaa caacgtttca aaggtttcga
    67321 tcatgccgaa taccgacgtg cgctccagtg catccacggt ttgcatgacc tgggggatgg
    67381 tcggcacgaa actcaaataa atgccgcccg agcgcaggac tttcgccgca tgcggcacca
    67441 cctgccaggg ttccggcaaa tccagcacca gacgatcgaa caagcggccg tcttccagca
    67501 catcgatgcc ctcgtaggca ttcttgcgac agggcatgag ggtagacacg ggccccatat
    67561 agcgttcgat gttggtcaat gccgtacggg caaaatcgtc acgggcttcg taggtgacga
    67621 ccaggccggt cggccccacg gcgcgcaaca acgccatggt gagcgctccg gacccggttc
    67681 cggcctcgaa tacccgcgcg ccgggataga catccgccca catcgggatc aacgacaggt
    67741 ctttgggata gagtacctgc gccccgcgag gcattttcag gacatattcc ccgaaggtcg
    67801 gccggagcgc gagaaaccgt ttgcccttcg agagggtcac cacggtgccg tccggcttgc
    67861 cgatgagctc atcgtgcgga atcgtttcgc cgctgaagtg atagatatcc ccggctttca
    67921 atgtcagggc atattgccgg ccctttttgt cgaccagatg aacacgttcg ccgttttgta
    67981 gttgtgacat agtccggcat tctaggagac tgccgacggg ttttcaaccg aatcaccggt
    68041 gacgcgcttc cggcaggccg cttcgccgcg cctcttgaca ctgcgaggag cggccctatc
    68101 tcgtcccatg ggcaatccgg attgcccgct gtctcgtaat accactgagt gaatgagtcg
    68161 agagcaggga cgaacgacgg cgaatccttt tcttaccccc ctgtctctaa tgtagtagtg
    68221 gaacaggcag cctctccaag ctgtaccata agcgaacctc cccaggacgg ggaagagggg
    68281 tgacaacaat gaaagatgcg ctcgcgattc aagacgatac agaccagacg gttgcggctg
    68341 aggtggaccc ggatgccgat gacccggaag ccagcacgct gctggacagc atcgtgcaag
    68401 gcaaccaggc ggagaccgaa gcagactccg aggactcggc gcctgagaag cctgcgcgct
    68461 cggcctcttc gccctttctc ctggaatccc tctacttccg ttcgttcggc gaacgcgcgc
    68521 tgctcgaacg ggatgaagag attgccctgg cgaaacaact tgacctcggc acacgccaaa
    68581 tccggcagac ccttcagcaa gcggtcaaag ccggcggccg cctgaaacga accgatcgca
    68641 cggccgagtg tctcgaaaca ctcaaaacag tgcgccgcct gagcggtctg tcggccaccg
    68701 tgctcaatca agcggaacgc gcactggaag aacttcaact ggacgccacc accggcggca
    68761 gagttcccgt ctcggtccgc agggaactgg atgcggcctt gacgcaattg aaagcctcgc
    68821 gcatcaccct ggaaacggcc aaagacgagc tggtgcgctg caaccttcgc ctggttgtga
    68881 atgtggccaa acattacacc ggacgcggcc tgaccctgtt ggatctggtc caggaaggca
    68941 atatcggatt aatgaaagcc gctgagcggt accagcaccg gaaggggttc aagttcagta
    69001 cctatgcgac gtggtggatc cggcagggca tcaccagatc cctggccgat cagtcacgca
    69061 ccatcaggat tcctgtgcac cagacggaag cttcgaaccg cattctcagg gcctcgcgcc
    69121 gtctcgtcca acagttagga cgacagccgc gcctggaaga agtggcctcc accatgcgga
    69181 tgcgccccga tcggctgcat gagaccattc aggccttcca ggaaccggtg gcactcgagc
    69241 atcgagtcgg agatggcggc acggagctgg gcgaactgct accggaccag caggcggtcc
    69301 cgccggacgc ccatgtgaac cgcacggagc tcacccgtga aatggatcgc attctcggca
    69361 ccttgacccc cagagagcaa accgtcatcc ggttacggtt cgggatcggg gaagaccagg
    69421 cgcgtacact ggaacaggtc gggcagcatc tgtcggtcac tcgcgaacgg attcggcaga
    69481 tcgaagccaa ggccctcaag aagctcaaga ccccgatggt caaggagatg ttcgcggcca
    69541 tcaaataacc acgacctgtt tcatacggca gcgcgatgcg ggcgagggtg acacctcgcc
    69601 cgtgttgttt gagccgcgct ttcgccatgc atcctattgt gagagaatgc ggcaacggag
    69661 gaacggctct cccctatgct gacagccctc tatagtcgca acggcgcccg gcccgatcgg
    69721 acacgatggt tcgcctgggg ccttttctca ctctccatcc tcagcctctg cacgccggcg
    69781 aacggcgcag gccacaatcc caatgaatcg acctcgctca cggaactgtc ggtacccgcc
    69841 gtcgtagcca aagtccgtcc gtcggtcgtc accgtgctca cgcgtggcat tccccaggga
    69901 ggctcccaac acggggcccc gccgggctcc ggctccggcg tgatcctcga tgccggcggg
    69961 cacatcttga ccaataacca tctggtgcag ggcgtgacca gcgtcgtggt gggactctcg
    70021 accggccggc tcacaccggg gcgagtcgtc gctcgtgatt ttctgctcga tctcgccctc
    70081 gtcagaatca ccgctccgga tctggttccg gccgaattca gctggcgcac gtccttcgaa
    70141 atcggtgaaa ccgtgatcgc catcgggaac cccctcgccc tgaagggagg ctccaccgtc
    70201 acggtcggcg tcatcagcgc actcgatcgc tcggtgctca cgccggaagg tgagaccctg
    70261 tacgatctgc tccaaacgga tgccgcgatc aacccgggga acagcggcgg cccgttggtt
    70321 gatcgatacg gtcaggtcgt gggcatcaat gtcgcaatcg ctccttccgc gcaggccatc
    70381 agttacgcca ttgctatgga ggcggtgacg ccccatcttc aatccatgat ggatcgcggc
    70441 tccgtcctgc ggccggacct cggcctgata ccgctgacga tcaccccgag tgtcgccgcg
    70501 agcttcgatc tggagagtga ccggggcatc ctcgcgctct cggtggatcc agccaaaccg
    70561 gccgggcgag ccggattgca atccggggat gtggtgaccg ccatcgataa ccatccgctg
    70621 tacaacatcg gagatttctg gcacgccgtg agtcgcgcaa gcgaccagtt gtcgtttcag
    70681 atcaccacgc aagggaaggc cggcacgggc accattactg tgtcacgtcc cggtccggct
    70741 cggccgtaac ccatgaaact cgatcagccc agccagacgg aatccgcgca cccggtctcg
    70801 aaggtgcctg cacaggctca gccgccccga gcgggcatcc tgatacgctg cgagcccctc
    70861 ccctacgaga tagcctggag tctgcagcgt gcctgccgga ccgagcgtgc ggccgaccgt
    70921 tgcgaggatc tgctcctgct cctggaacac cctcccgtgt ttaccgtggg ccgcacgacg
    70981 caagaccagc attggccggg agagagctgc ctgacgcagg aatcaggcat ccccgtgatt
    71041 cgcacggagc gcggcgggtc gatcacctac cacggaccgg gccagttgat cggctatccc
    71101 gttctccgcc tttccgattt ctgtgccgga ccgaaggcct atgtgcatcg attggaagac
    71161 gtgatcatca gcaccctggc cgagtggggc attgccggac atcgccggga gcggctgccg
    71221 ggagtatggg ttggagggga catcccgtcg aaaatcgcct cgatcggcgt gcgcatttcg
    71281 caaggcgtca cgacgcatgg tttcgcgctg aatgtctgcc tggacctggc gccgttttct
    71341 tacatcgtcc cctgcggaat tgccgactgc cgagtaacgt ctatggcggc cctcttagga
    71401 gaagcgcccg agatgggcac agtccaggaa aggttggctg ccaattttgc tgaacggttt
    71461 catctggcct gggcagaacg catcgggccg gatcggtttt tggctctgag cgacgcctca
    71521 ccgacgattg ctcaggggcc tctccctcag cgcgctactc accagcaagg agttacgtga
    71581 tgaacgaatt cgatacccac acctttcggc ttgccgttgc ccagtttaat gaagcggcag
    71641 aatccatgcg cctcgacacc aacctgcgtg agcgcttaaa actgccgcaa cgctccctgc
    71701 tggtcagcat accggtgaag atggacgacg ggcatgtgga agtgtttacc ggataccgtg
    71761 tccagcacga ttccgcgcga ggcccctgca agggcggcat ccggtatcat ccggacgtga
    71821 atcttggcga ggtggccgcg ctggccatgt ggatgacctg gaaatgtgcc gtggcggatc
    71881 ttccctatgg cggcgcgaaa ggcggcgtga aggtggatcc caaaaagctg tcccgcgggg
    71941 aactgcaacg cctgactcgt cggtacgcgg cggaaatttt cccgttgatc ggcccggaca
    72001 aagatgtccc ggcacccgat gtgggcacgg accagcaggt catggcctgg attatggata
    72061 cctacagcca gcaggtcggc tatgccgtgc agggggtcgt caccggcaag ccgttatcga
    72121 tcggcggcag cctgggccgg gaggaagcga ctggtcgcgg cgtctcctac gtcacactgg
    72181 aagccttgca gcacctgaaa ctggacgtat ccaaagcgac cgttgccgtt caagggttcg
    72241 gcaatgtcgg atcgaatacc gccctcatca tgcaacaagc gggcgcccgg gtcgtggccg
    72301 tgagcgacgt cggcggcgga ttgtacaacc cgaagggcct ggacattgcc gacgtgctcc
    72361 atcgctatcg tgacaaacac gaaccattgc gcgagatcaa gctgggcgag tcgattagca
    72421 acgaagaatt gctgcaactc gattgcacgg tactggttcc agccgcgctc tccgagcaaa
    72481 ttacccacgc caatgcatcc aagttgcgct gtagaattct ggcggaaggc gcgaacgggc
    72541 cgacgacgct agaggccgat cgcatcctga ccgacaaggg cgtgtttatc attcccgaca
    72601 ttctggcgaa ctcgggcggc gtgatcgtct cctattttga atgggtgcag gacgtgcagc
    72661 gcttcttctg gaaagccaaa gatattcaag accggctgca agacatcatc accagtgcct
    72721 tccatcgaac cctgcagttc tcggtggaga aacggacgac gatgcgcatg gccgcgttga
    72781 tgtcgggcat cgacaaggtg gcccaggcgc atctccaacg cgggctctac ccttagacga
    72841 caggatgccg aagacctcgc tcatgccatg acacgataca tactcgcggc gatcgccgga
    72901 atctggatgg ccgacggcct cgctttgctg acggtccccc tgctcgtcat caaaagagtc
    72961 caggagtccc tcctgcacag cccacaactg ctccgctggg aagccgtcgg tatcggcctc
    73021 ggagccattc tgatcctctg gtctgggccg attccctatc agccgctgtg gtggatcacc
    73081 ggtggcgcca tgatcatcaa ggggtgcttc ctcacatggg gacccgccgc atggcgcacg
    73141 ccgctgctgg attggtgttt cgcccgcgaa gccatcgact accggttctt cgggctgtgg
    73201 ttgtgcatgc ttgccgttct gctgctgcat gcgttagggt tgctccaccg gtaactacgc
    73261 ttacagcggg cttcgatcga gaccatcgtc atgagtcacc aaaccatcgc ctcactctgg
    73321 gaagagcaca gccgcgaggg ctggccgcgt ttctccagcc cgaacgaagg acaattgatg
    73381 acgctggata ccgtcatcgg gggatgtgcc gtgttttacc tcgacgggca gactgaaatg
    73441 gacagtcaac gcatcgcgat actccaggac tgcgtcgccg acctggatac cttgctcgat
    73501 gacctgactg aagacagctt gggctacttt caacgactcc gccgactcgc ggccgccctc
    73561 gtccagctaa atcaagcgac ctaaccgcga caccgcgagg cgcagcgggg accgaccggt
    73621 aggcctgccc ccgctgtctg ttccctatgg cacctcgacg atatccttct tcatcggacc
    73681 gaggatcccc aacaattcgc ttttctcacc ggccggcacg ttaaacttgt cgagcgcggc
    73741 gacaagatcc tgaaccaggg ctccaaagtc cgcactgctg atttgcatcc ccaggtgcgt
    73801 gcttttcatg tcgcgaccac gatagctgca ggggccaccc gtcgcgccgc acacttgatc
    73861 gaccaggtgc cccttcaact tggggatgtc cgtcgtagca aagcggctat taatccgccc
    73921 atccgccgcc acgttggcca caaattgatc gaccaccgcc gtaatggcag gctttcctcc
    73981 caaacggtca tagagggact ttgtggccgt cgcaccggcc gccgggcgtc cggctccgtt
    74041 cagactcccg gtatccgcgc aaccggccag caccgccagt gcactgagta caatcgctcc
    74101 cattccaaca cttttcgata ccatcatccg ccccccttgt aatatgtgac cgactgctgg
    74161 ccctcctcga cccaccatga gccgaggttt tcaaccagcc gcaccggttg accccagacg
    74221 ataggggaga tatgatctat tcgtctaata tattgttttc atatgtccta tagctttccc
    74281 ctatagcgag aagtaaaacc catcatggaa ttgcgtcaaa ttgagtattt catggccgtg
    74341 gcggcccacc aaaacttcag ccgcgcggca gaacatgtcc atgtctccca accttcttta
    74401 tcgatccaaa tcggcggcct cgaaaaggaa ctgggcacac gcctgttcga ccggctcgga
    74461 cggaaagtcg tcctgactca ggccggcgag ttgtttcatg tgcatgcgga gcgggctttg
    74521 cgggagctgg atcaggctga gcaggtggtg caagagctgc tcggcgcaaa acggggccgc
    74581 ttggtggtgg gcgcactctc aacggtcaac tcctatctca ttgccccgct ggtctctcgg
    74641 ttcaagcaac gtttccccga catccacctt caagtgcatg cccaggcctc ggcagatgtc
    74701 gtgagtgggc tgctgtccaa tcgcctcgac atcgggatct gcttgctgcc gctgacgcat
    74761 ccacaactga cgaccgtccc gctgtttgaa gaacgcctgg ccctcgtggc cccggcaggc
    74821 atgaacatcg ggaaacgtcg tgcgcgcatc caagacctcg cacggcttcc cctggtcctc
    74881 atgccggtcg attattgtct gcgcaaaatg gttgaagccg aatgcgccaa ggcgggtgtg
    74941 catccgcagg tggtgctgga aatgagttca cccgaaggaa tccttcaggc tgtagcggag
    75001 ggaacgggag ccacgattct tcccgagctc tacgtccgct cgcgactccc cggaatggat
    75061 ctacaggtga tcgatctcta cgatcccact ccccgtcatg ccgtcggctt ggcgtatctc
    75121 acgaagcgac accgcggact ggccgctgag gagtttgcag cgatctgcca cacgacgatg
    75181 cgggacctgc aatccgatcc cgaacagtcc aggcggaacc ggccaacaag gcgtgcagca
    75241 gggcagaaat agcatcccct ctatccgctc acctaaccgc taccgctcca actgaaagac
    75301 cggccgcgac cttgaaagtc agactgcccc ttggatagaa tgccatgttc tctgagtcat
    75361 ccccccgaaa tcctatacca actgtcagaa cggtgtgagt tctgaggatc tgttccaagg
    75421 aggccttcat gatgcagcgc aagtgggcgt tcacagcctt agtggcgatg atgggatccc
    75481 tttacctcgc tgccatggtg agcgccgggc cttatgaact cagcaaggac gacttgaccg
    75541 accccaaggg gattgcgagc ccggacgtgt ccctcttcgg aatcaagttg ggggatgatg
    75601 aaggaaaagc gctggagggg ctggtgaacg aaaagatccc cggcatcaag gccgaacagg
    75661 aagcgacgtt tattttcctg cttgatcaac gcaaaccgac cggcccgatg gccggggtcc
    75721 gggtacagga cggaaaagtc gatctcattt tcatcaacaa ccgctttgcc tacaaaactc
    75781 gtgggatctt tcgcaacgtc ctcaacagtg agagtccgga tgatgtgcgc aagttgctcg
    75841 gcaaagaaga ttacggtgat gaaaacgtca tgggcgcaat actcgcctat gacaagcagg
    75901 gcttccaggt caactatctc ggaaaagatg tgaacgtgga gttttcgctc ccccactaag
    75961 acagcccg
//
LOCUS       DAORRQ010000015        74404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_16_length_74404_cov_110.874121, whole genome shotgun sequence.
ACCESSION   DAORRQ010000015 DAORRQ010000000
VERSION     DAORRQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 74404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16_length_74404_cov_110.874121"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK224_06795"
     CDS             <1..245
                     /locus_tag="PK224_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15480.1"
                     /translation="SKKNAWLWLLPDEAYAGQGGGNGSKGKPRKERFLHPINSDYDLY
                     SMGKDGESVEPLTAQKSHDDVIRANDGSFVGLAIEF"
     gene            262..3021
                     /locus_tag="PK224_06800"
     CDS             262..3021
                     /locus_tag="PK224_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15481.1"
                     /translation="MQIESSFLRSRVARRVFALFILCALVPVGALSLVSLADVRSQLT
                     QQAERRVRQESKAMGMAVYQRLLLLEAELLSISRQHHAITPTAPGDVPTEPLPSTPGF
                     SGMARISGDGATSVFLGELQSIPTVSPAQWALLRQKKTLLTTATDDLGARRLFLVRLS
                     DPDTSAVVAQINGDYLWDLAGSMTLPAEMSMCVFGPAPEVLFCSPSAPATLKVQRVGQ
                     GEGGSSSLLEWRDEEDSYIAGYWAIPLRYQFSAAPWTVLLSESRSSVLAPLASFHYRF
                     SLVVVIALLCVALLSVNQIRKTMVPLEELGKGTRRIAKRNFSTPVQVKSGDEFEELAA
                     SFNAMASRLHQQFTQLATIHEIDRSVLSVLDPSRIVDTVLARLREVSPCQGLAVLLID
                     PSDRTRGWLYARLGPESKQTTMQGVTVTEAESRMLTAHRDGSTLLQPDGTGAFAALSG
                     QGSVSLLVLPLFRSHDLLGGIVLSYRTAPDMHADHLVQLRQLADQVAVALSNASDLAE
                     RKRAETSLRESHTQLEAAMTELKTTQQHMVQQERLRALGQMASGIAHDFNNTLSPIMG
                     FSELLLIAPQMAADPAQLKEYLQTINMAAKDAAKVVSRLRDFYRPRLDADLAGIVDLN
                     SLVQQSVKLSQPKWKDQALANGVAIEIKTELESVPSVAGHESELREVLTNLVFNAVDA
                     MPQSGSITLRTKVDGDAVRLEVSDTGTGMTEEVRQRCLEPFFSTKGEKGSGLGLSMVY
                     GIIRRLRGTIDITSAVGVGTTFSIRLPIQAEAETGTNHDSHNMDGVRLQILVVDDDPL
                     VGRVTGEYLRVAGHSVEVVGSAAEAIRRFSPERVHLVVTDHGMPHMTGRQLADVIKKI
                     SPDTPVLLLTGGDELEPDGTVSQAVDAELSKPLTMGALRQALSTLRYPKSLPAESQKE
                     IGDAA"
     gene            3076..4149
                     /locus_tag="PK224_06805"
     CDS             3076..4149
                     /locus_tag="PK224_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15482.1"
                     /translation="MSDLDASAVKLLLIDDELPSLKLMEAILKQAGYEKITTTTDPRQ
                     AVQLYREVKPDLVATDMRMPYIDGFEVMRQLTAVIPAEDYVPILVITGELDAPTKHRA
                     LAEGAKDFLNKPVDATEVVLRIKNQLVTRQLHRQLRMHNEHLEAQVRLRTKVVEQTQL
                     DLLNRLALAAEYRHDATGGHAWRVGRMAMLLAELKGLPADQVDMIKKTAPLHDIGKVG
                     LRDSVILKDGPYVMADWEAMKQHTKIGSRLLSDSRAPLLMMAREIALTHHERWDGAGY
                     HGIKDVQIPLAARIVALADAFDIMTHPCSYKATMTLSEAKSEIGAQAGKQFDPELSAL
                     FMQLIDREGEKLLAPTGPLQLVA"
     gene            4288..4707
                     /gene="nikR"
                     /locus_tag="PK224_06810"
     CDS             4288..4707
                     /gene="nikR"
                     /locus_tag="PK224_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HRB15483.1"
                     /translation="MKQLARFGVSLDQDLLADFDRLIERRRYTNRSEAIRDLIRDNLV
                     GQQWDENRETVATITFVYDHHVPGLTGKLTHIQHDFQGHIMAGMHVHLDHDHCLEVLV
                     AKGKGAAIRKVADALLSVKGVKHGKLTMTTTGKGLSI"
     gene            4707..5126
                     /locus_tag="PK224_06815"
     CDS             4707..5126
                     /locus_tag="PK224_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HRB15484.1"
                     /translation="MTICVVDGRGGGLGSRMVAGLRGLVDDGHAIIGLGLNSASAEAM
                     ARAGATFTETAPQMIHRRLYAADVIVGSLSLLMPGSMLGEVTPVLAQAVLESSARKVL
                     LPLNKRKVEVVGVEGRTLDALIDHALQRCVCLLQATA"
     gene            5253..7298
                     /locus_tag="PK224_06820"
     CDS             5253..7298
                     /locus_tag="PK224_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRB15485.1"
                     /translation="MRWIGWLIGAGAMVLSCGLVAFAHDPDAPDLDVPEVTVEADRPV
                     AASSQQFIPDKEYLLQPQGRPAQVLRLIPGFIAVEHSGGAGKADQYFLRGFDADHGTD
                     VAFFADGMPINLRSHAHGQGYTDLNFIIPETIEGLDVYKGAYLPEYGDFSTAGAVNFR
                     TREVVKEGVVQSAGGQFHTQRHLLMVSPTTDRVRSLIAAEGYYTNGPFEHDNRYFRGN
                     LLGKATMNPSARSELSVTGTFHKSQWNASGEIPLRAVQDGSLDRFGAVDPSEGGRTMR
                     STGRLNYHYDTTSGGRFFANAYAQYYRFDLFTNFTFFQNDPVNGDGFQQSDRRIMYGG
                     DIGYKQNGRWFDMDGAATVGVQTRVDHIHARLGPQVLRNPLGSTSDTDITEASYAPFF
                     KLEMQPAPWMRLAGGVRSEVFTFDVRNRCQTCVAQASGTADSGLVLPKANLTLGPWLR
                     TELFLNYGEGYHSNDARSAVTPAASPLARAKSYEVGLRSRPWGSDGIELTATVWALDM
                     KQELVFVGDEGTTEIRGASRRRGMEVGARGQVWGPVYFNGSITWTKAEFRNGDAIPLA
                     PEVTAYGALLLRWPEGLTSQLQATYLGVRPLTEDRSIRAPSWIDIDLSERYQIPIKLA
                     HGRLEAFLFVQNLLNTKWEQAVFAFESRLRNEATGVTDIHFVPGNPRMVMGGVAWYF"
     gene            7473..8042
                     /locus_tag="PK224_06825"
     CDS             7473..8042
                     /locus_tag="PK224_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRB15486.1"
                     /translation="MSRLNRKILDRSYFSCSTVEVARSLIGKYLVRNNGAGLMAGKII
                     EVEAYVGPEDKACHASKGRTARTEVLFGPPGIAYVYLCYGMHEMLNVVTEQDGYPAAV
                     LLRAVECDGSLIDGPGRLTRAFGIDRRLNRWDLTAGDRLWFEDRGDALPQDALKTYPR
                     IGVDYAGEWATKPWRFRLQTEAGSSRTKR"
     gene            complement(8119..8487)
                     /locus_tag="PK224_06830"
     CDS             complement(8119..8487)
                     /locus_tag="PK224_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15487.1"
                     /translation="MKFVIGSVATLAGVLFMCSMASANPALLPKHEGYPMKNDGSPVN
                     GQPTANDPGQKAGGGESTLLKSADSSSKHAEQKLVKSDNARITEGQGAGRLPNVQGPQ
                     IKIEPPVTSATKITGDRKID"
     gene            complement(8992..10824)
                     /locus_tag="PK224_06835"
     CDS             complement(8992..10824)
                     /locus_tag="PK224_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15488.1"
                     /translation="MSPPAESEKKAAADTHLQRTLTSVVNGLPADAALVAIFHQEQGP
                     LTTQVHRGFTPRDVQSIVRTLSSQKVLTSVPAGNDPEASRTMRLRLVTPGAKSLLGVP
                     LRHRQRTFGFLVIGRKDNAVYAKKDKTMLEQAGDDITKALERDSLFDLNVLLSRPLVV
                     QEPQPVIATPDVYNAPTSHATPEIQEKIVALLNELGQTLAFDRAWIGAYDPLAGNVEV
                     LGIAGEQKTDPKDQKKDLKAGQRLTLDASAAGWVVRHRKPRVDHDLASTQGRFLDHKH
                     LYKDRFQSSLVLPFFLRGQVGGTITLASKEADRFAVTDARLLEPINLKLVDLLQATPP
                     AAPGAAKAEGAPDSEAGSAALISAEPVIRKQERQAAIGEFSAFLATEIREPLGSIRAQ
                     LEEVTAEGILDFDPQTRVENAMRDLIRIEAILNEILDFAKPLDLTRRLCRVPEMVENA
                     LTVVATELEATRIQVVKEYAGLLAPVRADEAKMQQVFLSIFKNAIEAMTPGGILTISM
                     SNQRAGRHLEVQILIKNNGAPIPPEHVDKVFEPFFTTKRSGTGLGLATVKKIVEEHQG
                     SIGISGTPGEGTTVTIRLPGVSRGPAHRYRGRGRRPPRRPTAAS"
     gene            complement(11116..11574)
                     /locus_tag="PK224_06840"
     CDS             complement(11116..11574)
                     /locus_tag="PK224_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRB15489.1"
                     /translation="MRVFQLQGSQHSASSSGGLRPIVMRVLIMGLAVFLAVTIVPGIE
                     SESLGAGLAAVLVLTVLNTLIRPLLYLLALPLIVVSLGLFMVVINALLLQLTAALVKG
                     FTVVGFGASFWGALVISVVSSLLNMILVVEHTRVDRSDRPQRPPTIINPD"
     gene            complement(11747..12067)
                     /locus_tag="PK224_06845"
     CDS             complement(11747..12067)
                     /locus_tag="PK224_06845"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HRB15490.1"
                     /translation="MTFLRLFIAAFLLICAPAVVAAHGSGQHVLGVVAAIDATHIEVK
                     TPKGQVVSVRITDKTQYKVRNLRRPKSPPQVGDRVVIEAEKGTDGLTATEVHYSDSKP
                     KEAQ"
     gene            complement(12090..12707)
                     /locus_tag="PK224_06850"
     CDS             complement(12090..12707)
                     /locus_tag="PK224_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB15491.1"
                     /translation="MKKICGLLMLGFLATLGILGWFGYQSYSTGFSAKAEPNELEVLI
                     ARQLRHLAIPYENRQLRNPLPVTQELMKEARAHFADHCASCHANNGSGDTVIGKNVYP
                     KAPDMRLPETQTMSDGELFFIIQNGIRFTAMPGWGTGDPAKDSGSWQLVHFIRHLPSL
                     TEEELQEMTGLNPKTKKELQEEALFDQFLGGDDAAASKAAGSHHH"
     gene            complement(12815..13627)
                     /locus_tag="PK224_06855"
     CDS             complement(12815..13627)
                     /locus_tag="PK224_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRB15492.1"
                     /translation="MEILLLFFKTILLRPYVFAFLAAFLVSAIALIGWPRTWRFWLIS
                     WITAFVCEFSSTRNGIPFGWYFYNGSTVGQELYFSNIPFMDSISFSFLLFASYCLALL
                     LLLPVQSSSCADTTSRLPDLKFDLPLRTSWPVFALTILLFAFIDMVIDPVALRGDRWF
                     LGKIYYYPDPGVHFGVPMANYVGWAVVGAISLLIYFPLDRRLQHPLPDHRPSTTHRLL
                     LGGGLYYGVLAFNLAMTFWIGETLQGTTGLLMYVPVTAILLLRLLAPATQSA"
     gene            complement(13838..14821)
                     /locus_tag="PK224_06860"
     CDS             complement(13838..14821)
                     /locus_tag="PK224_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB15493.1"
                     /translation="MKALVTGATGFVGGAVARALVKTGIEVRVLSRKGADLQNLAGLP
                     VEQVDGDLRNPESLRRALTGCRQLYHVAAHYALWAKDPSIFYDINVTGTRTLLEAARE
                     VGIERTVYCSTIGAIGLPPGGGPGTEETPVSLEQMAGHYKRSKYLAEQEVLKLAREGL
                     PVVIVNPSAPVGEADVKPTPTGQIIVDFMKGRMPAYIETGMNLIDVDDVAQGHLLAMQ
                     KGRQGERYILGNKNLLLNDVFQILSRITGVKAPTIKLPRLAILPLAYANQWISNLTGV
                     PPRIPLEGVKMAKYKMHYDCSKAIRELGLPQTPVEVALEKAVRWFRSHGYA"
     gene            complement(14928..15851)
                     /locus_tag="PK224_06865"
     CDS             complement(14928..15851)
                     /locus_tag="PK224_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRB15494.1"
                     /translation="MSPTGQLTPTGLDIRQYLERKREEVDQYLQSVIPSAETMPTTLH
                     ESMRYSLFAGGKRVRPILAIAAAEAVGHVGKAVLPVASSLELIHTYSLIHDDLPAMDN
                     DDFRRGKPTNHKVYGEAMAILAGDALLTMAFELCARANAEHSLDAARQVQLIRELAVG
                     SGNLGMVGGQVLDIQAENKDIDLATLQSIHKHKTGMLIRAAVRMGAITAGAMPAQLDD
                     LTFYAEQIGLAFQIADDVLNVTGTREELGKNPNTDAQRGKKTYPTFYGVEGAKQLAEE
                     CVTRANDRLASFGSKADPLRELARYITSRKN"
     gene            16137..16454
                     /locus_tag="PK224_06870"
     CDS             16137..16454
                     /locus_tag="PK224_06870"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRB15495.1"
                     /translation="MGGTIGIELPKQQIAAFCEKWRIHQLALFGSVLRRDFRPDSDVD
                     VLVTFAPQADWKFEHLLEMKGELESLIGRTVDLVEKRLIDESPNYIRRKHILTHMEPI
                     YVA"
     gene            16444..16785
                     /locus_tag="PK224_06875"
     CDS             16444..16785
                     /locus_tag="PK224_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRB15496.1"
                     /translation="MWRDDAYLLDMLIGARRVLEFRADLTWEQFRQSGLHQHAIAKAL
                     ENIGEAAGKISEETRARLSTIPWKQVIAMRHRIAHDYFRLDLLRVWEIAVHDVPALIS
                     AIEPHVPPEHP"
     gene            17014..18606
                     /locus_tag="PK224_06880"
     CDS             17014..18606
                     /locus_tag="PK224_06880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRB15497.1"
                     /translation="MNWIGKEAVVNHHQQVPFHLLKDVPDLACGEPGEGNLIVQGDNL
                     VALKALLPYYAGQVKCIYIDPPYNTGNENWVYNDNVNSPLMREWLGKVVGKEGETLDR
                     HDRWLCMMYPRLALLRQFLRQDGVILISIDDHEYERLKLTMNETFGAENHVATFIWKR
                     RTATDSRNLNGISGDHEYILCYQRTAGFRVQGQAKDLGKYTNSDNDSRGPWMSDNLTG
                     LANPAERPNLHYEIVNPKTGISYKPHPSRGWLYGRERMAELIEDNRILWPRKREGRPR
                     LKRFISDMQSATTGFSTVLDAPGNVVATKELAEIMGPKAFAFPKPSQLIGLLIEQATS
                     PGDLILDSFAGSGTTGHATLTLNNVAQDTAPRRFILVEMEPKIASDITAERVRRVAEG
                     YKNAKGEKVEGLGGGFRYCELGEPLFDESGKIRETVSFADLARHVYFTETGEPLPRGR
                     VTKSPFLGECRGVGVYLLYNGILNDKTANGGNVLTRSVLAQLPKFDGQKVIYCAGCLL
                     GKDRLQAERILIRQTPYEIKVS"
     gene            18610..19203
                     /locus_tag="PK224_06885"
     CDS             18610..19203
                     /locus_tag="PK224_06885"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HRB15498.1"
                     /translation="MATRKKSPPPGSLEPLILVIRNQRVILDADLARLYGVTTKRFNE
                     AFKRNRQRFPHDFAFQLTTTEFGNLRSQFATSSLQHAESTEVHPRWSQIVITSHGGRR
                     YCPWAFTEHGAVMAANILRSERAVQMSVFVVRAFVRLREHVAANHAILKRLAEIDRTL
                     LEHDTALLDVYEKLLPLLQPPPDAPTRRIGFHSKGKS"
     gene            19200..21878
                     /locus_tag="PK224_06890"
     CDS             19200..21878
                     /locus_tag="PK224_06890"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRB15499.1"
                     /translation="MITLKDYQERVLESLGDFFRCTAQTKTPEVAFREVTRRFGEAAP
                     YFPVSAAGLGPDMPYVCLRVPTGGGKTLLACYAAGLAQRQFMRAERAVVLWLVPSNTI
                     LDQTADALRDPRHPYRRALELACGAVEVMTIDEALRLSRAAVDGQTVVIVSTIQSFRV
                     EDTTGRRVYDQNGAFSEHVMNVPPDRLGDLFPGADGKPKPSLVNALRLRRPIVIVDEA
                     HNARTDLSFATLGNVLPSCIVEFTATPAREKTPSNVLHRVSAAELKTAQMVKLPLRVV
                     TRHPSQRDQLLAEAFTLRADLERLAALEGQQTTEYIRPILLIQAERVDACEPLRERLV
                     REFGLSNDEVKISVGRLDELKGVKDIASPKCPVRVIITVEKLREGWDCPFAYVLCSLK
                     ETWSATAIEQIVGRILRLPNAKAKQHPDLNCAYAFSVSDSITAVLAELREALEHNGFT
                     KAEAERIILSVPQGTLPLGVQPQTVTVGPDEIDPTVVQVQEPALGGKVRIDAASGAIT
                     IVVPLDHDDLEKVQSCVTTPGAKARLAEAAEMVRQAEQAFGGTGKPRKPSPYEQQLDF
                     LVPLLCFAENGMLYEFESTFLLDHPWKLSEKDASLPAAYNPLARPYGKVGVIDVGQKG
                     DVQTTLLGDTGDADFVGTLHQQMFHFSGQDDWSVERLVAWLDREIDHHDIPVGESAEF
                     LRKVIRGLMAKYEIADIGTLALDRFRLRDEIAARIQDHREGERKASFQLLLLSDSPLT
                     VTDERTINFKTIGYEPSRVYEGGFQFQKHYFGSRPGELTEKTAEGRITEEFQCAQFLD
                     GLPQVRFWVRNLARKSTSFRLQTSKDWFYPDFLCQLTDGRTLAVEYKGKHLFDGVDAE
                     DKRAVGEIWASRSDGRCLFVMPTDGDFSTIRKMLAA"
     gene            21995..22228
                     /locus_tag="PK224_06895"
     CDS             21995..22228
                     /locus_tag="PK224_06895"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRB15500.1"
                     /translation="MTTLALTIEKQARKLSPTERERLAERLMAGMTGERLTSVDEAWV
                     AEAEQRFSAWKRKLTKPLSVAKSLRDIRKDLRR"
     gene            22225..22524
                     /locus_tag="PK224_06900"
     CDS             22225..22524
                     /locus_tag="PK224_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB15501.1"
                     /translation="MRLLLDPAAKAELSQAALYYEDCRHGLGEEFLVAVEAAFDQIRK
                     YPTLWRTLKGRFRRYLVQQFPYGVIYAVEGRTIYVAAVMHVKRKPGYWASRNKLS"
     gene            22576..22866
                     /locus_tag="PK224_06905"
     CDS             22576..22866
                     /locus_tag="PK224_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15502.1"
                     /translation="MSIRKAAGEWEPMLLGIEPRNCKVCQQGLYRDKTMLRGGWSRCA
                     VCDEFVHYSCLASGKISFLKQRPRICKTCRTAEEQAKQAVPPPSSQPAPVGS"
     gene            22863..23198
                     /locus_tag="PK224_06910"
     CDS             22863..23198
                     /locus_tag="PK224_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15503.1"
                     /translation="MTLLLDPQQRSTRPVWHVYFSHTLRYVLAPLLFISSGFFTANFI
                     VSGHYTWPRTSRVFVLTLTLLILAYEFIYKDHTVRSTSPERAFNAVLYSCMIPYALGV
                     LMMLGLAKL"
     gene            23355..23729
                     /locus_tag="PK224_06915"
     CDS             23355..23729
                     /locus_tag="PK224_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15504.1"
                     /translation="MGQRFQSGAFVQQCFAVHPLCLSLKRIDETGRLIIACTSCRMMH
                     HMAADQVVARVAAVPVDGDMSGAKALPTAKELLEACLQAHPVSVTIRELDVIRDSVGL
                     RCADCRRIFDLQVSVFESHQKS"
     gene            23837..24466
                     /locus_tag="PK224_06920"
     CDS             23837..24466
                     /locus_tag="PK224_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15505.1"
                     /translation="MASPSQDSSLTPAELTLVADGELFRAKERIILKVRDLLTELQAG
                     LQEDLSRTALLVPPGFDPSKMQVVKGERLEECPYQYLDYPKHFLGDDKFTFRSLCWWG
                     HHLVFAIIVEGGQAKQCKKNFFDRFHQLAGQGLELSLAPTLWEWKRGEGYTLPITHDR
                     KAQLAAVLSGRAWFKIARFVPLDQPAMSEGRLSEVGRETFRSLLPLLTP"
     gene            24636..25286
                     /locus_tag="PK224_06925"
     CDS             24636..25286
                     /locus_tag="PK224_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15506.1"
                     /translation="MIAQGCRMVGQCCVAVGLALLLGCAGTGEMVSLNLQTQQPFAQS
                     SQTEPLKIVIEPFEDSRTDRSKIGQRTHLGGGVTNFNVNGGAPGVTIAEALAETLRQR
                     GWNRRGWDARVVQAGVGVSGADIVIGGEIRDFSTNAKSRVFNTKLTGESRLVIKARNL
                     ADDSSTVRNIQGEQTKLVFWFTSEDVETLMSGLLQDGIERFITDTKIEGRTLKSAK"
     gene            25332..26393
                     /locus_tag="PK224_06930"
     CDS             25332..26393
                     /locus_tag="PK224_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15507.1"
                     /translation="MRERRWETSGRLTFREVLAVGERLATLGLKPAHPAKDVICYVED
                     WAVDSPDAFDQLDHWATEDVTLLHIREAWQGDFFLLAGGYHTVYQRFQDVGTYCSISH
                     PWRTKPGLRFHHPTCMFWLGFRHNHAFIRVRLHTQEVVTPGETREDARRVEWIDERRA
                     IFLDAIGTLDLPVETSVEKGGLVLRPADPATPFFCSWPDAFGPCQFEYNSLDAYEFLV
                     PASRLAATFAPQPAGVRVYLTGFSEPALDAFAAVQLGARSVYRCSVHCALGELPEVLA
                     TIEPQGRLYSTLCEFQTQELMPEGDDASAIIGIVGTAGGFQLEVRLNRAPLPEEHMAA
                     WLERLLGYDVTYAPLPPFM"
     gene            26637..27746
                     /locus_tag="PK224_06935"
     CDS             26637..27746
                     /locus_tag="PK224_06935"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HRB15508.1"
                     /translation="MVSMQRFQSVLGKPYLPPLFFFSGVTYDTVTLTRIDRLQDNLLL
                     LLYLTLLGFLIVLTGRLGTNEATVEDLPPDAPRLMRWIVQAKPYAPMAIQFLLGSLFS
                     AYAIFYSKSATFTGTAVFFCLLVGFLVANEFLRDRLSNVQLLVGLYALVCFGFFTFFL
                     PVMTGLMNQAIFLAGAALTVLVVLRVVQLIYWRNPARTNREALLAGAPAMGLIGLLVG
                     FYFLNWIPPVPLSMKFGGMYHEVKRAGDHFELSYEKRWFQVWQRSNTTVAADEPIYCF
                     TAVFAPVTLKTTVYHHWYYRSDKSKPFSHADKIPLKIAGGREGGYRAYTFKQGLDVGD
                     WRVDVETEDGRVIGRVPVHVEKRDDASRSLTTIVY"
     gene            28007..28876
                     /locus_tag="PK224_06940"
     CDS             28007..28876
                     /locus_tag="PK224_06940"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HRB15509.1"
                     /translation="MAVIVLVLAAWALPAIAEEPPQQALAWQVGPGEAKLGDQAVLKL
                     PKGYRFLGGQETQRLLKQMGNFPSGAELGLITATAENEQWFMVVRYIDAGYVKDDEAA
                     NWDADALMTSIKEGTDEDNKTRQAQGFPPLVIRGWEEKPHYDKAASKVVWAISAQERE
                     TVGVNYNTLALGRQGYLSMNMVGSLEQLPVLKPHVGLLLSNVEFIEGKRYTDFDSTTD
                     KVAAVGLSALIAGAAIKSGLLAKLWAFIIPLVIAGKKLLMLLVIALGGLAAKYFNKKP
                     KPEQAGGDGGLSS"
     gene            28873..29583
                     /locus_tag="PK224_06945"
     CDS             28873..29583
                     /locus_tag="PK224_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRB15510.1"
                     /translation="MKILLLLLGGLKLGKIALTGGTMLLSMVVYSFIFGWWYAVGFVL
                     LILFHELGHYAAAKQRNLNVGAPTFIPFVGAWIQLKEQPMDVETEAYVGIAGPVAGTF
                     AAMACYYVAEYTHSQLMLALAYAGFMINLFNLIPLSPLDGGRITAIISPKVWWLGVPI
                     LIGLFVMNHSPMLLLIAIMAIPHLMATFRGGPHGLPERYYDVPLSTRVSYGLYYLGLA
                     AFLGIMSYETHLLLPSRN"
     gene            29610..30317
                     /locus_tag="PK224_06950"
     CDS             29610..30317
                     /locus_tag="PK224_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRB15511.1"
                     /translation="MHTFSAEVSRIQDLTHDVRAIELRLLEPSSIAFKAGQFVSFEVP
                     KEGQPRPVTRPYSIASPPGQRDRILLVLNLVQGGPGSSYLFGLREGERASFKGPAGAF
                     YLRDEGVRDLLFVATGTGIAPLRSMILAQLERGESRSVTLFWGLRSQRVLYWQEELTA
                     LAADHPNFSFVTTLSRPDPGWEGASGRVTALIDAQVSSVGNLAVYLCGGSGMIKDVTA
                     RLNAKGLCPIYREKYYD"
     gene            complement(30469..30654)
                     /locus_tag="PK224_06955"
     CDS             complement(30469..30654)
                     /locus_tag="PK224_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15512.1"
                     /translation="MADTSTPQSNPSAPTAELPERLCTWCKVPMTKRLVAGGQFIHYT
                     CPKCVFQHTTKRAPKTE"
     gene            30808..31251
                     /locus_tag="PK224_06960"
     CDS             30808..31251
                     /locus_tag="PK224_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HRB15513.1"
                     /translation="MHNAEGATSDDISSSSARVADSAVHPDSVGPGKAWGICTSVDLH
                     DCIPERIRDAKQIEAYVVQLCELIEMKRYGACQIVNFGEGRVAGYSMVQLIETSLISG
                     HFANDTNSAYLDIFSCKGYEPAVVEEFSKAFFGARRSSHQAMLRY"
     gene            31296..31658
                     /locus_tag="PK224_06965"
     CDS             31296..31658
                     /locus_tag="PK224_06965"
                     /inference="COORDINATES: protein motif:HMM:NF026214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRB15514.1"
                     /translation="MPELYPMNPTPPKTVRAFFSTLAGKLDPEAAEGLDAVYQFNLNG
                     ADGGQYQLQIRDGACQVSEGTHPDPHVTLAMSGEDCLKVLNGQVSGTMVAMTGRLRIS
                     GDMGLAMQLASLFPSLRP"
     gene            complement(32102..32929)
                     /locus_tag="PK224_06970"
     CDS             complement(32102..32929)
                     /locus_tag="PK224_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15515.1"
                     /translation="MSSHHFASAIEMLDWARDAIHELKISCDAFFNANNFREVREFDA
                     DSNQNVLKIVLVSKLPQSIPRKANEALLNIRHSFDRSIYAACTAIGHPPKDSIYFPWA
                     DSPTDLDYKLGAKAKPGKTPKIRVPIELWDVLRELEPYGRSDAYAGGHNFIRELAKIA
                     NRKHTIRLNFGAAVSGIDFPSFSGSMKGPAKFELGNSRWDTMKNEIVLARFPSSIETD
                     YKYTLHMYVAFNESPPLKGANLIGALSDFLTYAQTVLVRLQGKAETIVGQEVHASPP"
     gene            complement(32949..33314)
                     /locus_tag="PK224_06975"
                     /pseudo
     CDS             complement(32949..33314)
                     /locus_tag="PK224_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_199855086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(33321..36500)
                     /locus_tag="PK224_06980"
     CDS             complement(33321..36500)
                     /locus_tag="PK224_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB15516.1"
                     /translation="MWLTLLALRNRIGILMLSLAMVILGATSLERLPVDLFPNIQVPV
                     AFVGVIYKGAPPLDIEQSVVYPIEKAVSSASNVEHVESFAKQGIGAVQVWFNWGADIN
                     VGQMEVMQRITQILNSLPPGILQPFIVKFDVSNIPVAFVTASGGDLDERALYDLAYNT
                     IAPQIEQISNVAAATVEGGKIRQININLDPALLQARGLSILDVVSAVKASNLILPSGD
                     IKAGNLDYNVFTNTQFKTVEPINDVVVKIDQRGNPVRVRDVGVLSDSSDIQTNVVRTD
                     GKRSVYLRVNKQPIANTVEVVDALRKAIPKMIGIPPGVQLGISFDQSIYIRQSIKNLI
                     EQALHGSLLAAAVILLFLRNLTSTLIISVAIPLSILVTFIVLYFTNQTLNVFTMGGLA
                     LGIGRLVDDSIVELENIQRHLNVDRNRWDAIVNAAREVAMPIFASTVTTVVVFLPMFF
                     IVGIARLLLIPLTLTIAIALFTSFFVSRTVTPALCYRFLKSEQEAHRSLPSWFVSIMQ
                     WSQRRYDALDEGYERSLRWVLAHRRTLLVSVVTIFVGSLALLPFIGTEFLPVSDESQF
                     RIVLRGPVGQRVEKTVDQVAEVERVLREKIPPDELEAIASSTGVLAQGRSSLFNPNTG
                     PHTSVISIYLSSPDKRRRNQVEIMNAVRPAILKLFPGVAMFFDPGGLVKRVTSFGSQK
                     SIDVEIYGYDFEKARTVIHQVQDLMHKIPGMADIEVSREENYPEVNVVVDREKAALLG
                     ISETDVANAVLFSLNGNGQTDPIIFTDPQNGNEYYISAWLAKEHRKDLSAIEHVLLTT
                     KNGEPVLLKNLATLKLNAGPVKIERKYFQRVVHITANPVGRDLGSIAQDLESAFAQMQ
                     LPPGFSIRLAGQIQQQRETFEGLTYASVLALILVYMVMAAQFKSLIDPFIIMFSVPMG
                     IPGVIVILYLTNTTISTSSLMGIIMMLGIVVSNGVLLVDYTNVLRRRGLALSTAVVTA
                     SRTRLRPILMTSLATVVGLMPMALGLGTGSETNAPLARAVVGGLTVSTILTLFLVPTV
                     YTMLEERFPRRADQLGEERVQSELVSQQA"
     gene            complement(36585..37778)
                     /locus_tag="PK224_06985"
     CDS             complement(36585..37778)
                     /locus_tag="PK224_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB15517.1"
                     /translation="MSVNLIRRHPIVTLGIMLFVAVVALVALRLSSGAKSDPRKNRIL
                     TVGTMSPIKQDLDVRLTYTADLIPNQLVNIFSRVDGYIAKIYVDKGDLVKTNQLLVEI
                     DHTDYVHAVNQAKANLLSAKAKVVQQEAAVRNAALTLDRMQALIKDQFVSQQDLDTAL
                     VNRDAALALQDSLRAQVQQMDVALAQAVTNLAYASIRAPFAGYIAERNLDPGAYVSGT
                     TASTSTMSRGILSVHDVETVRTLIEVVEKDVPLVKVGQRADVRAEAYPNEVFEGTVTR
                     IVQALNRATRTMTVEVDLPNKDHRLKGGMFARVEVLVGKHPQAIQIPLDAVSRLEESQ
                     YVYVVKDGKAHQVPVELGARAENRVEVVKGLTGDEQVIVSGKDLVSEGAAVQTQPMDT
                     VKRES"
     gene            complement(37768..38415)
                     /locus_tag="PK224_06990"
     CDS             complement(37768..38415)
                     /locus_tag="PK224_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRB15518.1"
                     /translation="MKPTNALSRNPGQDRQASLISSAASLFAAKGFKGTTTKAIARAA
                     GVSEALVFKYFPTKRALYTAILAEKAPLSELLHAVEEVARKRDDQRVFTLIAGYRIRP
                     GADPTMLRLLLFSALEGHELADMFFGKQHRVFYDYLAGYIRTRIEEGAFREIDPLLAA
                     RAFIGMVVHHRLLHEVFDVPLHCPHKEIVSTYVELFLNGLRRSSTPRKRGLSRVR"
     gene            complement(38539..39078)
                     /locus_tag="PK224_06995"
     CDS             complement(38539..39078)
                     /locus_tag="PK224_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15519.1"
                     /translation="MAQLLFITPGQWPTALTAFVAALLLPVALALAVPMQNDPKGFEG
                     IPWGASFSESDTFVKVEDTGRVQTYELKSADRTLGSATVDSMKFSTVDRKFARVMVRY
                     SGKEAHDRILSYLQERFGPLDQTPGQVAGGAVKFFNWQGDESEIILRYDLRTSQGVIF
                     FESEAFRAKFNEGNSPSTF"
     gene            complement(39080..39622)
                     /locus_tag="PK224_07000"
     CDS             complement(39080..39622)
                     /locus_tag="PK224_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15520.1"
                     /translation="MPYAFSKCSPLRMASRLGLLVVLIFILTVPALAITMANDPHGFD
                     HLTWGVPLTDIPELTVTRSNSHTIDYQFRDRPPVYADIPVESVHLSTVDDQFARVTIR
                     YSGAKAHAQVLQFLERSYGKIERLPGQMMRGLNQQYTWRGPDTEISLTYEANRDRGFI
                     FIESRNLAPRFQDRMSDTAE"
     gene            39765..40262
                     /locus_tag="PK224_07005"
     CDS             39765..40262
                     /locus_tag="PK224_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRB15521.1"
                     /translation="MIESDVFVQALENLGVNFFTGVPDSLLGGIIEELLTRKLYTPAV
                     REDEAVAMAAGAYMAGKVPAVLMQNSGLGTSLNTLISLNMMYRQPCILLVSWRGFEGK
                     DAPEHLVMGETMPQLLDTMRIPHRTLSEPTMMDDLRWVGQTVMKQRVPVALLIKKGII
                     KGLHP"
     gene            40259..40837
                     /locus_tag="PK224_07010"
     CDS             40259..40837
                     /locus_tag="PK224_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB15522.1"
                     /translation="MRPEQGTMQSRAQAMAALLELLADQPVIICNGFPSREAHKLADR
                     PTHFYMIGSMGNAPAIALGVALSKPGKQVIVFDGDGNVLMGMGTLATVGALKPKNFIH
                     VVFDNEVYGSTGNQPTISNVVQLEKVARAAGYVNVERVLDRDDLVYEFKDMLKKDGPS
                     MLLVKVNEFVEDAGRVPYEPPAITARFMSAIE"
     gene            40907..42001
                     /locus_tag="PK224_07015"
     CDS             40907..42001
                     /locus_tag="PK224_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRB15523.1"
                     /translation="MILLNPGPVNVSERVRQALLRPDICHRESEFSDLLGRIQQKLLA
                     AFVPGAESDYVAVLLTGSGTAAVESAVMSCLPMGKRMLVLNNGVYGERLSSMIGLHRL
                     GVSELKSEWHIKPDPERVRLALRQHPEVHAVSMVHHETTTGLINPVKEIAEVVDSLNR
                     VFVLDSVSGLAGEPIDIAGSHIYMVAGTAGKCIQGFPGVSFVLLRKGFLERMRNYPKR
                     SWYLHLTHYVDDQGKGIVPFTPAVQIYYAFEEALNELLEEGVANRCQRYKRMAVRIRE
                     RMANLGVKALLPADSLSNTITAFHLPPGISYATLHDQLKARGYVIYAGQGQLESKIFR
                     IANMGALTELQIDGFLAAFEEVVTGAVARA"
     gene            41998..42717
                     /locus_tag="PK224_07020"
     CDS             41998..42717
                     /locus_tag="PK224_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HRB15524.1"
                     /translation="MKAIILAAGVGKRLWPVTQHKPKCLIEIGGQTLLSRYLESLASV
                     KIRQATIVVGYKQEMIRAAVGSHCHGVDLSYLVNEEFHRGSISSLWIARTALSDDVVI
                     MDADVLFHREILRRLVASPYENCLLMDDTVKQTGEECMVVVQGDRVVALSKKMPERYD
                     IAGEGVGFLRVRRADTAHVVGSLKGYIDQDRWDMEYEDGLLQYFQDVKVGHEKIGGLP
                     WTEIDFPEDVTKAEREVLPRL"
     gene            42839..44290
                     /locus_tag="PK224_07025"
     CDS             42839..44290
                     /locus_tag="PK224_07025"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRB15525.1"
                     /translation="MSESTLQRGAELQGLSTAILLPGAGLFGDRPGRGLTDVGPLTSI
                     GGLSLFQRTVLTLQRGGMRQLIVLAGSDEELLKHALARGARVTIPVRWMPVREFPLDD
                     PRTWESLATEVRGFCLIAGVQAVFSKGLIEHLRQSVRDGEALVVTREAGPVEPALGRR
                     NPAVALQEGRLISFHNHPGQEGHQVAADLVVLPASILTPPNGAAASPSGAAEPAGMIP
                     VRRWLERAAVEGRVRVVAAAAHAGLWYRDVWDHTSAGLAERTLFRSLKGEAEGFVDRY
                     FNRTFSRLLTRLFLRMKCSPNAITMVATAVGILSAVGFGIGTYSAGIVAALLFQLAAV
                     IDCCDGEVARLTFTESPFGAWLDIAMDNVVHIAIFAGIACGAYLRQAGTEGAWVPLAL
                     GGAAVFGNIMSFWLVTKAQKIGATRGWNTPKQAAWSDFILKNVASRDFSVVVFLFALL
                     DKLDWFLWMAALGSTVFWLMMVWVIRPSARARA"
     gene            44283..45290
                     /locus_tag="PK224_07030"
     CDS             44283..45290
                     /locus_tag="PK224_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRB15526.1"
                     /translation="MLKAALLLLGALTFSVLVWHIGIGRIYEAVTQLGPAAMLVILLP
                     SLLMYILEAYGWLVTLGAWAKDVPFWRVLAIRTAGEVVNMTTPTAYVGGEPLKAFLLK
                     RQGVPIVEGLASVVTAKTTMTIAQILFILAGIGLGFWLLGAGGSAGQTITAGLVSVGL
                     LVFGIGAFVIVQRQGMFAWMLNLLRRIGLRLQYLESREQKLLELDRTIAHFYAHRRTA
                     FLVSTGLFLLGWLAEALEVFVMIVCLGQPITVLSAIAIGALSVFIKGGTFFIPGSLGA
                     QDAGNLFLLTAFGYGDVTGITFALLRRFRELVWIGIGLACLVVVGKGQNDTLEDPAAQ
                     R"
     gene            complement(45847..46786)
                     /locus_tag="PK224_07035"
     CDS             complement(join(45847..46443,46445..46786))
                     /locus_tag="PK224_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611132.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HRB15527.1"
                     /translation="MDAYSQDLRDRVLRALERGERPTAIAARFEVSRVWVYQVRNRLT
                     QTGQRSSLPIGGHRQSRLVGMEPTLRAWLKETGDLTLAEMCARLAAHGIAMKVPALWH
                     QLDKWKLTRKKTLHASEQDRADVHAARREWQAAQPALDITKLVFLDETGASTNMARRY
                     GRAPRGERCVASVPHGHGHTTTFVAGLRHDDISAPMVADGAMTGTLFLKYVQEFLCPT
                     LHPGDIVIADNLRSHKVAGVKEALEAVGAHIRYLPPYSPDLNPIEKLFAKLNALLRKA
                     APRTVDALWAEIGMLLDRFTPAECTRYFASSGYVKT"
     gene            46883..47131
                     /locus_tag="PK224_07040"
     CDS             46883..47131
                     /locus_tag="PK224_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15528.1"
                     /translation="MRIAHQLTFPPGTIVAMDRGYLDYVLYHRWTLAEVGFVTRPRTN
                     MLHEVLERRPVPMRGPMVADEVIRLTGLQAAVPSLCGR"
     gene            47128..47718
                     /locus_tag="PK224_07045"
     CDS             47128..47718
                     /locus_tag="PK224_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB15529.1"
                     /translation="MTIWDETQQRRLTFLTNLMHLAASTIAAIYKERWQIELLFKALK
                     QHLKIKTFVGTSENAVQVQIWIALIAMVLLKYLRLKSTWPWSLSNLAALLRFNLLTYR
                     DLWTWLNAPFKHPRSLRCPCKQGFLPHNLGQHPDQTTSFENRRRSRLKRSIHAGDTQR
                     GGLFWTAVASSPLCLPQVSRQMPQACRRGESAGCAP"
     gene            48353..50212
                     /locus_tag="PK224_07050"
     CDS             48353..50212
                     /locus_tag="PK224_07050"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRB15530.1"
                     /translation="MPAVNPRDQKVVIIGGGPAGLTAAYQLSKVGAKSVVLEKDAILG
                     GISRTVEYKGYHFDIGGHRFFTKVSVVDKMWREVLPEGDFLHCQRLSRIYYNRNFFFY
                     PLKPVNALFGLGVFNSLLILMSYVWAQLFPQKPEDNFESWVSNRFGKRLYKTFFKTYT
                     EKVWGMPANTIAADWAAQRIKGLSLLVAVKNALIKQQSTEKGAVVKTLIDAFDYPKRG
                     PGMMWEIVGELVKKDGSQVHLGASVDRIRWAGNRIDGMDVSMQGRNESLTGTHYISSM
                     PIRELIHKMNPPAPKEVIAAADRLRYRDFLTVALIVNKKDLFPDNWIYIHDSQVKVGR
                     IQNFKNWSPYMVPDQNKTCLGLEYFCFEGDGLWTMSDKDLIELGMRELELIGIAKAED
                     VEDGSIVRMPKAYPVYDADYQEALTCIREFLDGFQNLQVVGRNGMHRYNNQDHSMLTA
                     MLAVDNIQGARHDLWQVNAEQEYHEETTSREADAKVQLGQLAATQPQVPQPMTPAASQ
                     SLVLAFAKLDKLAFATALGTVSGLIVAIATLWIAVKGGERAGQNLQMLGQFFQGYTVT
                     VSGALVGFAYSFTSVFLWGWLFAYLRNFALGFVIYRARREAELMSFRQFLDRY"
     gene            50240..50578
                     /locus_tag="PK224_07055"
     CDS             50240..50578
                     /locus_tag="PK224_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15531.1"
                     /translation="MPTMMQLEDEKLFSAILRINSKMFGLILGLLMGLAIFIATNWLV
                     FKGGYTDETGHYIVGPHLQLLSQFFIGYSVSFVGSFVGFAYGFACGTLAGASIGIIYN
                     KLVQLRNSAS"
     gene            50587..51630
                     /locus_tag="PK224_07060"
     CDS             50587..51630
                     /locus_tag="PK224_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB15532.1"
                     /translation="MKFGLIGAGCIGQLRAKALAQVEGCVLSAIVDVDPVRARAMASS
                     SDVKIFKDVQEMLPQANLDAVIVSTPPQFHEDLVVSAFKAGKHVLCEKPLANSVEACR
                     RMVDAARASGKVLATGFNHRYFPQIQYLKQAIDTGLIGELDHVRAFAGHTGLSEFRNS
                     WEYDINGIGGGALMDVGIHIIDLTRYVLGEVDEVYGFTSNRVWNLGASEDNGIALLRN
                     PQGKYAVLQATWTEWKGYRFYLEAYGTKGMVRAYYAPMMNLLITQDKPGGRRKRTVRF
                     YPMTMLREKFIGWQSSVIQMFRQEFTDFVRLTRGKESAIADGVAGLRAVEIANAVYRS
                     SKEHIAIRLAGNI"
     gene            51656..52588
                     /locus_tag="PK224_07065"
     CDS             51656..52588
                     /locus_tag="PK224_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB15533.1"
                     /translation="MTTVNPNHAEFKTQDAASYNPVAQSFDRFVTQLSAPVAARLVAL
                     AGLAPDNHVLDVGAGTGIVALRAVAEMGPSGRVVGIDLSDGMLEVARQNARGRGLADR
                     VEFRKMDAESLDCKDGSFDAVVSLFALRHFPHPETALQEMHRVLRPGGRLVLAVGSGA
                     PWLSLVGLVHRLKLLPHVVRRFQGKQLVACKFLDGMVAAYVPSSGKSEEAGWTHEHTH
                     MQQSVPAFIRAAGFQNIRSEWQGQQSIINTPEEFWELQTTFSSLSRKRLATVSSEVVA
                     RVRQDFMNTCREVLSRGGELVYPTGALLVSGRRA"
     gene            complement(52627..53076)
                     /locus_tag="PK224_07070"
                     /pseudo
     CDS             complement(52627..53076)
                     /locus_tag="PK224_07070"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            53405..54076
                     /locus_tag="PK224_07075"
     CDS             53405..54076
                     /locus_tag="PK224_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15534.1"
                     /translation="MSKRVIRQLCIAAISGVLTVGLFVAVDSKDATFRWSMATAYVGL
                     ALIGLSLIIGPINVLRGCSNPLNTSLRRDVGIWGGIIGLVHTVVGLQVHMAGRFWLYF
                     LYPREESHLVPLRYDLFGLANYSGLGISLVLALLLGLSNNAALTKLGSHRWKTLQRWN
                     YAGFALLIVHGAVYQLLEKRMAGFVLVFAAAILLVGALQTAGYRKVLQQKNPGGQPSV
                     MSSDR"
     gene            54330..55466
                     /locus_tag="PK224_07080"
     CDS             54330..55466
                     /locus_tag="PK224_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRB15535.1"
                     /translation="MRTLVVNAFRMAQQRTGIGRYIEFVAQQWSRMKIPFDRVVLMSP
                     KNIEMSGFDRVTSVEVKVFGGQYPAAIWEQVCFHRAAKGAAILFCPSYICPLLHRGRM
                     VLANHGFYEALPGEFSWFTRLRATTLQRYSAKCAERVIANSLATKADLVRFFGIPPEK
                     IDTIYPAAHDYYFQRHDPAAIKEEVIRALGKEVPYVIFVGKLAKRRHVPNLIEAFAIV
                     RRQHNLPHALLIVGPNTTNVPIAEIAQQHGVGDAVVYRPYAELEPLARLYAGADVYAL
                     PTIYEGISYTMFEAMASGAAVLTVKHPTLEEGAGDAALAMPTPSVEDLVEGLSTFLLD
                     GAVRRKYAEQGRERAKRFSWEMVARETMRILDRVADKEDRNGYC"
     gene            55519..56991
                     /locus_tag="PK224_07085"
     CDS             55519..56991
                     /locus_tag="PK224_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15536.1"
                     /translation="MLKILLGIGAPQALAVLVNFARTKVAAVLVGPEGIGVISIIDQT
                     IQFVANLCTLNFSRSTVKFLSKAHSEGGDAFCDGYAVVLKALLVLGTVGALVTTSLVL
                     FRIDLLPAVIAPYKILLLVGLLGIPGMALASFIPNALAAAQYSSGSARLTALTGLVMA
                     LASTFGLLAGGILGFYVATVLIVTVWAIVVVEYLRRVMGWSITQCAVGIAAGFRRLGN
                     VVSFSILFYAAAFTYQGAFLITRYSVLENLGEAGAGLLHAGMSVALLMNMVLMPLTEL
                     FFTPRVTRVGPKEEKFQVAMEFLKQLTMIVTIFSLPAILFPHLVLDLAFSTKFLGAAQ
                     YMFVFMLWQSFALLSGVCQALLIGLDDMISYTAITCSGNVLAACSAVVLVPHYGGIGA
                     AVGFVIASSLVLLGTFVRLKLWHGFALPRNLVFLMGYSLLVTLVLGSIFRHYGAWHGS
                     TLLAKAAAALATVIGLLLFLSRNDKLALVSFFQKVRSGPW"
     gene            57327..58775
                     /locus_tag="PK224_07090"
     CDS             57327..58775
                     /locus_tag="PK224_07090"
                     /inference="COORDINATES: protein motif:HMM:NF028498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRB15537.1"
                     /translation="MNSNNRFQEVAKKISVVLLNGVLLVAMPACASVNLGRDSMPTMV
                     EVTIFPQEPRQTMEGFGASSRVWDDPHVSNAGSTSVPAAAQENVLTLLYTDLGLTRMR
                     PVLDGGVEPINDNVDPAAFDWTKFHFEWKRTDAHISLVEQARAKGMTTFFVSPVTLEA
                     WMTEDNPDEYVEWALAIMLRWRDRGVELPYFSIINEPGYKRSGIWSAEWMRTVVKRLG
                     ARMRQEGFRTMLVIPDDLNPAEAYKRASVVLRDSKARRYVGALAYHLYDTKETELAHD
                     LAHMRNLAAEYGIPIWMTEFSVPNDMTFSGAMEWMKIIHKLVATYDVSAVDYMWGFFG
                     AWERNHTILSIEFLDGKYVQHHVGPLYYLTGQFSKFVRPGQVRVETHSSSQDLLVTAY
                     KSGAQSVIVAINAGQEDRRVNVTVTDAVAPLHFSAIRTTVREFWSELEPIASDTSTFN
                     VALPAQSVTTYRGGSIDSAGTLSGVGIKKSAQ"
     gene            58772..59875
                     /locus_tag="PK224_07095"
     CDS             58772..59875
                     /locus_tag="PK224_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB15538.1"
                     /translation="MIRLAVIGLGAVTRNIHLPAYRRLKGRVTLVAGCDVDLATRAAM
                     EKTGLFKRIYDSHEEMLDKAKPDVVSICTPPFLHHQQCLDALERDCHVFCEKPLAESL
                     AHADEIILASSRCMRSVVVNSQFPNMNIYTAAKKQIGSPEFGRLLFLHAWQTFRPTAD
                     TEGGWRANMQRRLCFEFGVHVFELIRHFFDATPSTLWAHMPRPIRASNTESLNIIAME
                     FPDGRAASILLDRVSKGPERYLEMRLDGEHAAVHTSIGGELRFEVGLHTRQRKPFMGL
                     HVVKGGQAVLQQAERATVIGTDGMNPFASATAVHFGNFLSAIEDGRVPSGTAEDHKAT
                     LALTLAAYDAAESRKVVAIAPYLSGAATRKDLS"
     gene            59916..60971
                     /locus_tag="PK224_07100"
     CDS             59916..60971
                     /locus_tag="PK224_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB15539.1"
                     /translation="MATGPIESLAIENRRLRVGIVGAGLMGQWHAYEARKAQGNLVAI
                     ADCNLEAASRLARTFQGVEACVDIEELLSRTSCDLVHICTPIATHEQIAGTALEAGKH
                     VLIEKPATPDAEGTQRLIQLAARRGVLICPVHQFVFQQGVMKARRLLDRIGHVLHLEA
                     IFCTAGGVGRQESELDSVVAEILPHPLSLAQVFLPGGMVPDTWMRLRPRVGEFRAMAE
                     VSGVTVSVMISLQARPTQCTLKIFGAAGTIHLDLFHGYAFIEPGAVSKERKILRPFDL
                     AARRLAAASINLAKRALSRERAYPGLTRLVAEFYAAIRTGQCAPFTTEAMLNIARVRD
                     ILVEGMSRDGYTAVSHS"
     gene            60971..61885
                     /locus_tag="PK224_07105"
     CDS             60971..61885
                     /locus_tag="PK224_07105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15540.1"
                     /translation="MATSPGASAQDLPELSVVIGSHNARTSVAACLGALQKQRNGCRA
                     EIIVVDNSTDGTAAIVAAQFPDVALMCTSPEQWIPELWEAGIRASRGSIVALTTAHCV
                     PDDDWMKEVLAAHHGSAYVGIGGAIENDASASITDWAVYLCRYARYMRPFAPYVIRDI
                     PADNASYKRWALDSCTDVRARGFWEPDIHAKLTAEGYQLFMTPRIGVRHLRSFTFIKF
                     MRQRFWHGRQFGSSRAMRLSTAVRLAHIVAAPLVPFLMLTRIACQVFRRRQHITHLLL
                     ATPVLLLFLASWTTGQVIGYLAPTGHQA"
     gene            61882..62796
                     /locus_tag="PK224_07110"
     CDS             61882..62796
                     /locus_tag="PK224_07110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15541.1"
                     /translation="MIGQESLKNPRLSVIVACTHSRLDLELVLSSLRGQEGADGLEII
                     VVDSSGDEAANQSIVQDEGVLTLRFPRGTPLPALWGAGIRQSRGAIIAVTDACSVPDR
                     RWVLSTQAAHESSDLIVGGAVDPMPQRSLVDWAAYFCEYGQFMLPLTGGDVSELPGNN
                     IAFKRAALDKGTQFVEQRFWKTYWCRALQQEGVQLRSVPSMVVYDRKSYRLLPFLVRR
                     YHHGRCFAGMRITQVPPVIRVLYALGTPLLPVLFMRRIAGPIMQKRRYLPQFMCSLPV
                     SILAVVSWSWGEFCGYLAGPGRSCEFIQ"
     gene            62826..63722
                     /locus_tag="PK224_07115"
     CDS             62826..63722
                     /locus_tag="PK224_07115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15542.1"
                     /translation="MGNRSISVVIACVNRLPSIHDCLIALERERKNFQVEILVLCCCQ
                     DGTAAHVAKHFPDVTLVHFPRRLGIPELRAIGMERATGEIISIIEDHCIVQNGWFREI
                     LAAHDKGYQVVGGTVVNGSVERLIDWAVFLCEYSGVMPPIPDGEVSGVTGNNVSYRKA
                     ILDRVDESVKKECWEFFLHQELSKKGVTFLSASSIIVSHKKEFGFWYFMAQRYYYSRS
                     FAGMRRKRTSLPGRLLYCCASPLLPGLMLWRMTRQVLNKQQHVGAFVWSLPLQAAFMV
                     SYAAGEFVGYLLGSGDSLLKVE"
     gene            63719..64630
                     /locus_tag="PK224_07120"
     CDS             63719..64630
                     /locus_tag="PK224_07120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15543.1"
                     /translation="MNNAPAVGIELSVVLAAAHSRAGLEACLTSLKDQVSGGEIDVIV
                     ACNCCEGAGRSIEEAFPFVRLIEAPPGTTVPELRTLGIQAATGRIVALLEDNSIVAPT
                     WCRAIRRAHAAPQEIVGGAVERDGTHRAVDWAVYFYEYGKYMLPCVEGVSATLSGNNV
                     SYKRTLLQEIQDEFRNGFFEAFLHERLRRQGSTLYMAPDAVVYHTLRYRVGKVLVQTF
                     HHGRHYAGRRVAAASRLTRLGYAAGSTLLPVILPLRIAQLVLHRRRHLRELTVAMPYL
                     VLFMTSWACGELMGYLCGEGESVRRWA"
     gene            64651..65856
                     /locus_tag="PK224_07125"
     CDS             64651..65856
                     /locus_tag="PK224_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRB15544.1"
                     /translation="MGPSTRKPRIGVDACCWSNRRGFGRFTRELLTTLVATDREHEYI
                     FFADGDTARASEFPPGVTVVVASTEVSPTVAASASGSRSLKDLWALSRVVAQEPVDLF
                     YFPAVYSYFPILNRCKVIVTIHDMIADHHPERVFPNRKSRLFWKLKQQAALWQSDMIL
                     TVSSHAKQQIVEYCRVPATRVETTTEGPSDVFIRLPNLEGATAVLPKYGLRSGEPFLI
                     YVGGISPHKNLGVLVDAFHRLMEDPRFADHKLVLVGDYKGDVFFSHYAELKADIEQRG
                     LSARVLFTGFVPDSELAVLYNLARALVLPSLEEGFGLPAVEAMACGAPVIASDRGSLP
                     EVVGQVGLFFDPSRPDVLVTLLRDVLSSESIRDRMSLAGLERAKLFTWELAAQSTAAL
                     FERVLNEVR"
     gene            65856..67112
                     /locus_tag="PK224_07130"
     CDS             65856..67112
                     /locus_tag="PK224_07130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB15545.1"
                     /translation="MGPSLRFCMVTTFYPPYSFGGDGMFVYRFSNLLAERGHSVDVIH
                     CRDAYYAQHATEPVGEWPNHPRITLHSLKSKAGILSPLLTQQLGIPGLKAGKIKRILT
                     EGRFDVIHYHNVSLVGGPKVLEYGCAIKLYTTHEYWLLCPTHVLFKFNQEACTKPSCL
                     MCQLSCRKPPQLWRYTGIMDRALRQLDAVISPSQFAADAHRHMGLNVEKTVIPMFVPS
                     RQAEQERGEFLPASLPDGQLPYFLYVGRLEKLKGLQEVIPFFAQQDQADLLVAGTGQY
                     ESELKRLAQDSPRVTFLGALPFERLRQLYAHAVAVIVPSLCYETFGQVVAEAFSFKTP
                     VIARDIGALSELVTTSGGGLLYRNGHELGDAIESLRTNPELKAELGKRGHEAYRKQWT
                     EEEHLDRYLALISRIGERKRAEHAMV"
     gene            67166..68320
                     /locus_tag="PK224_07135"
     CDS             67166..68320
                     /locus_tag="PK224_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB15546.1"
                     /translation="MRYRWKSLLVNLSLLLGSLALSLIFCEAILRLVAQNDSPLSMDI
                     YQLDPYDQVSLKPNVTRRHFTREWNVTVAINSEGLRDRPSPVPDTRGRILALGDSFAF
                     GWGVELPETYLYLAEEQLRSEAIRVIKAGIPGTGTTDQLHWLRQYGGTYAPRLVIVSL
                     FVGNDFTDVQMGGFKKQFTIKDGLMVRRSLDGTERSSPAREAIEKLKRTSLLAQRLAQ
                     VLWYLQNTFVAPQNRSNPGLNAVDRWLWEFAKVHLRQPPPETVEAFARTRTALDDIHQ
                     WCRQNNAEMLLLVQPRSMQIYDWELAKWKEAYRINDEALDLDRPQRFLADWAAEKHVA
                     ILDLLPEFRAYQKSHSAERLYFYPDSHMNRIGNQLTGVLLAKWLNEFGFQ"
     gene            68800..69555
                     /locus_tag="PK224_07140"
     CDS             68800..69555
                     /locus_tag="PK224_07140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB15547.1"
                     /translation="MTPYRLPILTYHSLDSSGSVISTTPSIFRRQMEWLHQQGFQTLS
                     LAEAARLVRDGQPFRERTCVVTFDDGYETVYREAFPVLEELGFTATVFLITGYCGKQN
                     SWPGHVSPVGEQLLLSWTQIEEMARHHIEFGSHTVTHPDLACVTRHEAAREMRESKQT
                     IQERLGGDVEVFAYPYGKYASWVVDLAKEHFTGACSTILGKVTSEADSALLPRVDMYY
                     LSSQWLMESLDTRGMDLYLGVRRAMRATKAWFA"
     gene            69582..70598
                     /locus_tag="PK224_07145"
     CDS             69582..70598
                     /locus_tag="PK224_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB15548.1"
                     /translation="MLQVEEFCCPTCKGVLSFQESEYACSACSKKYPVILGIPDFRVF
                     PDPYIDFEDDHKKAGHLAEQYHKLDFMGLVRLYWKMTPEVSEDRAERFIRRTAALVEK
                     GAECLSLIESLAETEKLRGFNRVLEIGCGTGGFLVAAKRKYDHVVGADIAFRWLVVAR
                     KRLEELKMNVPLVCCCAEYLPFPGTRFDIILAEDVLEHVRSQESTLAECRRVMTKQGM
                     LFLATPNRFSLTPEPHVRVWGVGFLPRRWMNGYVKLVKGISYENIKVLSFMELRRLLN
                     KCLFTNHRILLPSISCEEAQYFSPFQRMQLAIYNVAKQIPVLRLILYVVGPYFNVVVF
                     SSRK"
     gene            70621..71610
                     /locus_tag="PK224_07150"
     CDS             70621..71610
                     /locus_tag="PK224_07150"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB15549.1"
                     /translation="MGNKNFLSVVVPVYNGGWCLGQCLAGIQRSSYREYELIVVDNGS
                     TDNSVAIAQAHGALVAHCPGPSGPGAARNTGVKLATGQILLFVDADVVVHNDVLNRVV
                     ARFIEDTELAAVFGSYDDSPPAKNFLSQYKNLLHHYVHQQANNRATTFWGGCGAIRKD
                     VFVNVGGFDQEKYPTPSIEDIELGDRMHRLGYRIWLDKGLQATHLKEWRWKSLLRAEI
                     LYRAIPWSRLIHERQGLVNDLNLKLSHRASAAVAGLFLAMLPLTPVFPPLAYGCLFLA
                     SIFLLLNRDLFVFFLRRRGVLFSAVTVPMHGFYYIYSGVTFVLVWLSYALHLR"
     gene            72106..73653
                     /locus_tag="PK224_07155"
     CDS             72106..73653
                     /locus_tag="PK224_07155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRB15550.1"
                     /translation="MTFRQLLQRVAPTDAQAPSRFESCILVLWYVTCANVLVLFLMQG
                     GYDVQVGPIHVHATSLRNWLALCFALTLSKAWLEERRGARSTFEGMQSPLLLFLGAVT
                     VYYSNGHTFETGDTFPARFLPVSLLTDHDFYLDEWASTIHLYEDQYFVRLVNGHLVSS
                     YPPWGAILALPVYVIPVLKGAGAGMFDLEKRAAVLIMALSVLILFLALRRVTRPRVAW
                     FVAFVYAFGTNCLSLNSQALWQHGPSQLFLSLTLYCLVRGVETPVFSAWAGLPLGVAV
                     MCRPLNLVMAVPIVLYIVHKHRGQCIGFMIAGVPPLLLFLWYNAVHFGSPLQTGFGAT
                     VVSPSSLVGHHLSWFNTPLFEGLAGVLFSPARGLFIYSPIFLCALAGMVVVWREPEQV
                     LLKYLSLAPLFLLVPVAMLGSWWGGLCYGPRLLADSTPFLCFLLGPALDRIHQRVWLK
                     YVVVGLAGLSLGMHAIGFAYSGDWNSDLIDFDRHPERVWYWRESPPVIFAKQLCTQMW
                     QKLAALL"
     gene            complement(73669..73806)
                     /locus_tag="PK224_07160"
                     /pseudo
     CDS             complement(73669..73806)
                     /locus_tag="PK224_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023662037.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            73862..74347
                     /locus_tag="PK224_07165"
     CDS             73862..74347
                     /locus_tag="PK224_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRB15551.1"
                     /translation="MQRQGMFGWILKMLRRLGLRIHYLESREQQLLDLDRTIAGFYAT
                     QRSAFLLSTGLFFLGWLAEALEVFVMILCLGQPVTVLSALAIGALAVLIKGGTFFIPG
                     SLGAQDAGNLLLLTAFGYGDVTGITFALLRRFREFVWIAIGLACLAMLPKGEEPPVPA
                     P"
BASE COUNT        14808 a        20082 c        22991 g        16523 t
ORIGIN      
        1 agtcaaaaaa aaacgcctgg ctgtggttgc tgcccgatga ggcatacgca ggacagggtg
       61 gcggaaatgg cagcaaagga aaaccgcgca aggaacgctt tctccatccg atcaattcgg
      121 actacgacct gtacagcatg ggcaaagacg gcgagagcgt ggagccgttg acggctcaaa
      181 aaagccacga cgatgtcatt cgagcaaatg acggcagttt cgtcgggctg gcgatagaat
      241 tttaacgaag agtcagccac ggtgcagatc gaatcttcct ttctccgcag tcgtgtcgcc
      301 cgccgcgtgt tcgccctgtt catcctctgt gccctggtgc cggtcggcgc gttgagcctg
      361 gtgtcgctcg ccgatgtcag aagtcagctc acgcagcagg ccgaacgccg ggtgcggcag
      421 gagagtaagg ccatgggcat ggccgtctac caacggctcc tcctcttgga agccgaactg
      481 ctctcgatca gccgacagca ccatgcgatc accccgacag cgcctggtga cgtcccgacg
      541 gagccgcttc cgagcacacc ggggttctcg ggtatggccc gcatttcggg agacggcgcg
      601 acgagtgtct ttctcgggga actgcagtcg attcccaccg tatcgccggc gcaatgggcg
      661 ttgctccgcc agaaaaaaac gttgttgacg acggcgaccg acgacctcgg agccaggcgc
      721 ttgttcttgg tcaggctttc cgatcccgat acctcggccg tggtggctca aatcaacggc
      781 gactatctgt gggacctggc cggcagcatg acgcttccgg cggagatgtc catgtgtgtg
      841 ttcgggccag cgccggaggt cctgttctgc agcccgtccg ccccggcaac gctgaaggtg
      901 cagcgggtcg ggcagggcga aggaggcagc tcgtccctgt tggaatggcg agatgaagag
      961 gattcctata tcgcaggtta ttgggcgatc ccgctccggt accaattttc ggcggcgccc
     1021 tggaccgtgc tgttgagtga atcgcgaagc tcggtgcttg cgccgctggc aagctttcac
     1081 tatcgattca gcctggtggt cgtgatcgcg ctgttgtgcg tggcgttgct cagcgtgaat
     1141 caaatccgga agaccatggt gccgttggag gagctgggga agggaaccag gcggattgcg
     1201 aagcgaaact tttcgacgcc ggtacaggtc aagagcgggg atgagttcga ggagctggcg
     1261 gcctccttca atgccatggc gagccggctg catcagcaat ttacgcagtt ggcgacgatc
     1321 catgagatcg accgatccgt gctgtcggtg ctcgacccca gccggattgt cgatacggtt
     1381 ctggccaggt tgcgcgaggt ctctccctgc cagggcctgg ccgtattgtt gatcgatcca
     1441 tccgatcgca ctcgtgggtg gctctatgcc agactcgggc cggagagcaa gcagacgacg
     1501 atgcagggtg tgacggtgac ggaggctgag agccggatgc tcacggcgca ccgggacggc
     1561 tcgaccctgc tacagccgga cggaacaggg gcgtttgccg ccctgagcgg gcaagggagc
     1621 gtgtccttgc tggtgctgcc tctcttccgc tcgcacgatc tgttaggcgg gattgtgttg
     1681 agttaccgga cggcgcccga catgcacgcg gatcatctcg tgcaattgcg tcaattggcc
     1741 gatcaggtgg cggtggcgct gagcaacgcc tcggatcttg cggagcggaa gcgggcggaa
     1801 acgtccttgc gggagagcca tacgcagttg gaagccgcca tgaccgaact caagacgaca
     1861 caacaacata tggtccagca ggagcggctg cgggcgctcg gccagatggc gagcggcatc
     1921 gcgcacgact tcaataatac cctgtcgccc atcatgggtt tcagcgaact cctgctcatc
     1981 gcgccccaga tggcggcgga ccccgcgcaa ctgaaggaat atctgcagac gatcaatatg
     2041 gcggccaagg acgcggcgaa ggtcgtcagc cggctgcggg atttctatcg cccccgcctc
     2101 gatgcggatc tggccggcat tgtcgatctg aacagcctgg tccagcagag tgtgaagctc
     2161 agtcagccga aatggaagga tcaggccctg gcgaacggcg tggccatcga gatcaaaacg
     2221 gagttggagt cggtgccctc ggtcgccgga catgaatcgg aattacgcga agtgctgacc
     2281 aatctagtgt ttaatgccgt cgatgcgatg ccccaaagcg ggtcgattac gctccgcacc
     2341 aaagtcgacg gcgatgcggt gcgcctggaa gtcagcgaca ccggcacggg gatgacggaa
     2401 gaagtgcgtc aacgttgtct tgaaccgttc ttctccacga agggcgaaaa ggggtccggt
     2461 ctgggattgt ccatggtgta cggcatcatc cggcggttgc gtggcaccat cgacatcacg
     2521 agtgccgtcg gcgtagggac gacgttcagc atacgcctcc cgattcaagc ggaggcggaa
     2581 acaggcacaa accatgacag ccacaacatg gacggggtcc gcttgcagat cctggtcgta
     2641 gacgacgatc ctctggtggg ccgtgtgacg ggcgaatatc tccgggtggc cgggcatagc
     2701 gtagaagtgg tcgggagtgc ggcggaggcg atcaggcggt tcagtcccga gcgggtgcat
     2761 ctcgtggtga ccgatcacgg catgccgcat atgacgggcc gtcagttggc cgatgtgatc
     2821 aaaaaaattt ctcccgacac accggtgctg ctgttgaccg ggggcgacga actcgagccg
     2881 gatggcacag tgtcgcaagc ggtcgatgcc gaattgagca agccgctgac gatgggggcg
     2941 ctccggcagg cgctgtccac gctccggtat ccgaaatcac tccctgcgga gagccagaaa
     3001 gaaattggtg atgcggcatg aggatggtag ggtggaagcg gtaggatttc gagaaccagg
     3061 agcacgggtg ttgccatgtc ggatcttgat gcgagcgccg tcaaactgct gctgatcgat
     3121 gatgagctgc ctagtctcaa actgatggag gcaatcctca agcaggccgg ctacgaaaaa
     3181 attaccacga cgaccgaccc gcgtcaggcg gtgcagctat atcgagaggt gaagccggat
     3241 ctggtcgcga cggacatgcg tatgccgtat atagacggat tcgaagtcat gcggcagttg
     3301 accgccgtca ttcccgccga agactatgtg ccgattctcg tcattaccgg cgagctcgac
     3361 gctccgacaa agcacagggc tttggcggag ggcgccaagg atttcctgaa caagccggtg
     3421 gatgccacgg aagtggtgct ccgcatcaaa aaccaattgg tcacgaggca gctgcatcga
     3481 cagctgcgta tgcacaatga acatttggaa gcgcaggtgc gcctgcgtac caaagtcgtg
     3541 gagcaaacgc agctggatct gctgaaccgt ctcgcgctgg cggcggaata tcggcatgac
     3601 gcgaccggcg gccatgcctg gcgggtcgga cggatggcca tgttgctggc ggagctgaag
     3661 gggctgcctg cggatcaggt ggatatgatc aagaagaccg ctccgttgca cgatatcggg
     3721 aaagtaggcc tgcgcgattc cgtgatcctg aaggacgggc cctatgtgat ggccgattgg
     3781 gaggcgatga agcagcacac caaaatcggc tcgcggctcc tgtcggatag tcgggctcct
     3841 ctgttgatga tggcccgcga gattgccctg acgcatcatg agcggtggga tggagccggc
     3901 tatcacggga ttaaagatgt gcagatcccg ctggcggcgc ggatcgtggc gctggccgat
     3961 gcgttcgata tcatgacgca tccctgctca tacaaagcga cgatgacgct cagcgaagct
     4021 aagagtgaaa ttggcgcaca ggccggtaaa cagttcgatc ccgagttgag cgccttattc
     4081 atgcaactca tcgaccgtga aggcgagaaa ctgttggccc caacgggccc gcttcagttg
     4141 gtcgcgtagc ggttctcttt ccggttcgat tcctcccgct gggagcgcct caccaaacat
     4201 tctcctcacg gccgcttgac gcattcgcgt ccggctttgt attatccgtg ccacgataat
     4261 caatttcgta acacggacat cacggcgatg aagcagcttg cacgattcgg cgtctcgctc
     4321 gatcaggacc ttcttgccga tttcgatcgg ctgatcgaac gtcgccgcta taccaaccgc
     4381 tccgaagcga tccgcgactt gatccgggac aatctggtcg ggcagcaatg ggacgagaat
     4441 agggagaccg tcgccacgat cacgttcgtg tacgaccatc atgtgccggg gttgacgggg
     4501 aaactcaccc atatccagca cgattttcag ggacacatca tggccggaat gcatgtgcat
     4561 ctcgaccacg atcactgcct ggaggtgctg gtggccaagg gcaaaggggc tgccattcgc
     4621 aaggtggccg acgcgttgct gagcgtgaag ggcgtgaagc acggcaagct caccatgacg
     4681 acgacgggta aaggactcag catttgatga cgatttgtgt cgtggatgga cgcggcggcg
     4741 gcctgggaag ccggatggtc gccggtttgc gagggcttgt ggatgacggt catgccatta
     4801 tcggcctggg gcttaatagc gcctccgcgg aggcgatggc tcgagccggc gcgaccttca
     4861 ccgaaacggc cccgcagatg atccatcgcc ggctgtatgc tgcggatgtg attgtgggct
     4921 cgttgagtct cctcatgccg ggatcgatgt taggtgaagt cactcccgta ctcgcgcaag
     4981 ccgtgctgga atcgtccgcc agaaaggtgc tgctccccct caacaagcgg aaggtcgaag
     5041 tcgtcggtgt cgagggacga accctcgatg cgttaatcga ccatgccctg cagcgttgtg
     5101 tctgtctgct tcaggcgacc gcctgatcga aaccgccgat ttacatctca gattgcacag
     5161 tcagatcacg agggtctgaa gcggccgcta ggggcgacgc gtggaccatt ctgagcgcct
     5221 agttcgctca tgtgatgtga ttgggagcga ttatgcggtg gattggatgg ttgatcggtg
     5281 cgggagcaat ggtgttgagc tgcggcttgg tggcgttcgc ccacgatccc gacgcgcctg
     5341 atctcgacgt gcctgaggtg actgtcgagg ccgaccgtcc ggtggcggcc tcgtcgcaac
     5401 aattcatccc cgacaaggaa tatctcctcc agccgcaagg gcgcccggcc caggtgctcc
     5461 gcctcatccc gggatttatt gccgtcgaac attccggcgg cgccggcaag gccgaccaat
     5521 attttctgcg cgggttcgat gcggatcacg gcacggatgt cgcatttttt gccgacggca
     5581 tgccgatcaa cctgcgttcc catgcgcatg gccaggggta taccgacctc aatttcatca
     5641 ttccggagac gatcgagggc ctcgatgttt acaagggggc ctatctgccg gagtatggcg
     5701 atttctccac ggcgggggcg gtcaatttca ggacgcgcga ggtggtgaag gagggcgtgg
     5761 tgcagtcggc cggcgggcag ttccatacgc agcgccacct gctgatggtc tccccgacga
     5821 cggatcgtgt tcgctcgctg attgctgccg aggggtacta cacgaacgga ccgtttgagc
     5881 acgacaatcg ttacttccgg ggcaatctgc tcgggaaggc gacgatgaat ccgagcgcca
     5941 ggtcggaact gtcggtgacc ggtacctttc acaagtctca atggaatgcg tccggggaga
     6001 tccccttgcg cgccgtgcag gacgggtcgt tggatcggtt cggcgcggtc gatccttccg
     6061 agggcgggcg aacgatgcgc agcaccggcc ggctcaacta tcactacgac acgacgtcag
     6121 gcggacggtt ctttgccaat gcctatgcgc agtactatcg cttcgatctt tttacgaatt
     6181 ttacgttctt ccagaacgat cccgtcaacg gcgacgggtt tcagcagtcc gatcggcgga
     6241 tcatgtatgg cggagatatc ggatacaaac agaacggacg atggttcgac atggacggcg
     6301 ccgccaccgt tggggtgcaa acccgggtcg atcacatcca tgcgcggctc gggccgcaag
     6361 tgctcagaaa tccgcttggg agcacgagcg atacggatat caccgaagcc tcttacgccc
     6421 ccttcttcaa actggaaatg caaccggcac catggatgcg cctggccggc ggcgtacgga
     6481 gcgaggtgtt taccttcgat gtgcgcaatc gctgtcagac ctgcgtagcg caagcatccg
     6541 ggaccgccga ttccggtctg gtgttgccca aggcgaacct gactctgggg ccctggttga
     6601 gaacggaact ctttttgaac tatggcgagg gataccatag taacgatgcg cgctcggcgg
     6661 tgactccggc cgcctcgccg ctggcgagag ccaagagtta tgaggtcgga cttcgatcga
     6721 gaccgtgggg ttcggacggt atcgagctga cggccacggt gtgggcgctg gacatgaagc
     6781 aggaactggt gtttgtcggt gacgaaggga cgacagaaat tcgtggcgca tcccgtcggc
     6841 gcggtatgga ggtcggggcg cgcgggcagg tctgggggcc ggtgtatttt aacggcagca
     6901 tcacctggac caaggcggag ttccgtaacg gcgatgccat tcccctggcg ccggaagtga
     6961 cggcgtatgg cgcgctgctg ctgcggtggc cggaaggact aacctcccag ctgcaagcga
     7021 cgtatctcgg tgttcgcccg ctgacggagg atcgcagcat ccgcgcgccc tcctggatcg
     7081 atatcgactt gtccgaacgg taccagattc cgatcaagct ggcgcatggg cgactcgaag
     7141 cgtttttgtt cgtacagaat ctgctcaata cgaagtggga acaagcggtc tttgcctttg
     7201 agtcgaggct caggaatgag gcaacgggtg tcacggacat ccacttcgtg ccggggaatc
     7261 cgaggatggt gatggggggc gtcgcatggt atttttagca ggctgctgaa aaagcccgcc
     7321 agcgtcgttc tcgcctcggt accgtcctca acgtagccaa tgggctacgc ctccggcggt
     7381 accttcggct gcggccttgc tgaacgggct ttttgagccg cctgctatcc gatcggcgtc
     7441 aactaacatg gggggaacat aaaggattgc caatgagtcg actgaaccgg aagatcctgg
     7501 atcgttccta ctttagctgt tcaaccgtcg aagtcgcgcg gtcgttgatc ggcaagtatc
     7561 ttgtacggaa caatggggca gggttgatgg ccggaaagat catcgaggtc gaggcctatg
     7621 tcggtccgga ggacaaggcc tgccatgcgt cgaaaggacg aacggccagg acggaggtgc
     7681 tgttcggtcc gccaggaatt gcctatgtgt atctctgcta cgggatgcac gagatgttga
     7741 acgttgtaac cgagcaggac ggctatccgg cggccgtcct cttgcgggcg gtggagtgcg
     7801 acgggtcgct catcgatggg ccgggccggc tgacccgtgc gttcggcatc gatcgccgat
     7861 tgaatcggtg ggatctcacg gccggggaca ggctctggtt tgaggatcgc ggcgacgcgt
     7921 tgccacaaga cgccctcaag acctatccgc ggatcggtgt ggactatgcc ggggaatggg
     7981 cgacgaaacc gtggaggttt cgattgcaga cggaggccgg ctcaagtcga acgaagcggt
     8041 aggcgcgatc atgggacaaa aagaaacggg gccgttgtct cacgacaacg gccccgtttt
     8101 cgttctatcg aatagaaact aatcgatttt acggtcgcca gtgattttcg tggcagaggt
     8161 caccggcggc tcaatcttga tctgcgggcc ctggacgttc ggtaaccggc cggcgccctg
     8221 gccttcggtg attcgcgcgt tgtccgactt caccaatttc tgttcggcat gcttgctgga
     8281 ggaatcagca gacttgagca gggtggactc gccgccccca gccttctggc ctgggtcatt
     8341 agctgtcggc tgtccgttca ccgggctgcc gtcgttcttc atcggataac cttcgtgctt
     8401 cggcaacagg gccggattcg cggaggccat cgaacacata aacaatacac ctgcaagggt
     8461 cgcgacgctg ccgatcacga atttcatacc cctactcctt tgaaagaatg tgaaagtatg
     8521 gtggtcgatt atagacgtta aaagcctgat gagtgcggga atcttagcta tctgaagaag
     8581 ccgtgtcaag gataaagggg ggaagggagc ggatggcctc aatgaccacc cacaggtgtg
     8641 tatggcgatt cgctacgaga agcgcggccc tagaagcccc tcgttggacg cgcgcaggag
     8701 gaggctcgcc acgccagtcc tgttgagggc gaatcacgtc gatgtgctcg gccgatgcgc
     8761 gccgatggat ggcactcagg accagagcat aaatggttgt aggaaaaaga gaacagctag
     8821 ctcaagcgac cacggttggg agaaagaaaa cagacggaca cgatctatgg ccggccatgg
     8881 aggacattca cacgaggaat cgtgtgagcg ctgggacaaa gtggcgcggc ttaataaggt
     8941 agcctcgcga ggcctgacct gattgatcag accgggacag gaaacaaggg cctaggatgc
     9001 ggcggtcggg cggcgtggcg gacggcgtcc gcgccctcga taccgatggg ctggacctcg
     9061 gctgacaccg ggcagacgga tcgtcactgt ggtcccttcg ccgggtgtcc cggaaatgcc
     9121 gatactgccc tgatgttcct cgacgatttt cttcacggtg gcgaggccca ggccggtgcc
     9181 ggaccgcttc gtcgtaaaga acggctcgaa caccttgtcc acatgttcgg ggggaatcgg
     9241 cgccccgttg ttcttgatca ggatctggac ttccagatgt cgtcctgccc gctggttgct
     9301 catggagatg gtcaggatgc cgcccggggt catggcttcg atcgcatttt tgaaaatgct
     9361 gaggaagacc tgctgcattt tggcttcgtc ggcccggacc ggggcgagga gccctgcata
     9421 ctccttgacc acttgaatgc gggtcgcttc cagctctgtg gccaccacag tgagtgcgtt
     9481 ttcgaccatt tctggaacgc ggcagagccg gcgcgtgagg tcgagtggct tagcgaagtc
     9541 gaggatttcg ttgagaatcg cttcgatgcg gatcaggtcc cgcatcgcgt tctcgacacg
     9601 ggtctgcggg tcgaagtcga ggatgccctc ggccgtgacc tcttccaact gtgcgcgaat
     9661 cgagcccagc ggttcgcgga tttcagtggc gagaaaggcg ctgaactctc cgatggccgc
     9721 ttgccgttcc tgttttctga tcaccggttc cgccgagatc aacgcggctg aacctgcctc
     9781 ggaatccggc gcgccttcag ctttggcggc gcctggtgct gcgggtgggg tagcctggag
     9841 caggtccacc aatttgagat tgatcggctc gagcaaccgc gcatcggtca ccgcaaaacg
     9901 gtccgcttct ttcgaggcca gcgtgatggt cccgccgact tggccgcgca ggaagaaggg
     9961 gagcaccagc gacgactgga accggtcttt gtacagatgt ttgtggtcga ggaagcggcc
    10021 ctgagtcgaa gcgagatcgt gatcgacacg cggcttgcgg tggcgcacca cccatccggc
    10081 agcggaggcg tcgagcgtca ggcgttggcc cgctttgaga tctttcttct ggtctttcgg
    10141 gtcggtcttt tgttctccgg cgatgccgag tacttcgacg ttgccggcga gcgggtcata
    10201 ggctccgatc caggcgcggt cgaaggccag ggtctggccc agctcgttga gcagcgcaac
    10261 gattttttct tggatctccg gggtcgcatg ggaggtcggc gcgttgtaga catccggggt
    10321 cgcgataacc ggctgcggct cttgcacgac cagcggacgg ctcagcagga cgttgagatc
    10381 gaagaggctg tcccgttcga gggccttggt aatgtcgtcg ccggcctgct ccagcatcgt
    10441 cttgtctttt ttggcataga cggcattgtc tttccgtccg atcaccagga agccgaaggt
    10501 gcgctgacgg tgccgcagcg gcacgcccag cagcgacttg gcccccgggg tgaccaggcg
    10561 aaggcgcatg gtgcgtgagg cctcaggatc gttgccggcg gggacggagg tcagcacctt
    10621 ctgcgaggac agcgtgcgga cgatcgattg aacatcgcgt ggcgtgaacc cgcggtgaac
    10681 ctgcgtcgtg agcggcccct gttcctggtg aaaaatcgca accagggcgg catcggcggg
    10741 taagccgttg acgacggagg tcagggtgcg ttgcagatgc gtatcagcgg cggctttttt
    10801 ttctgattcg gctggaggac tcatcgacct ctatgcagga tgctaccgac tacgcagggg
    10861 gctaccgatt ctgtcccggt atggatgact cagatggtga gtcataaccc gcagcatgtt
    10921 cggaacatgg tgctcgttat aggttgtgcg ctctcggcgg tgaatagcga gcgtcgggcg
    10981 gcattgtagc agccggatca aggtaactca aggaagcaca tgaatttgaa gagcacatgg
    11041 ccaatagctt atggcaaggt ccatggggcc tcccgccgtt tgtgctatac gccatacgct
    11101 atcagctctc cactgctaat ccgggttgat gatcgtgggt gggcgctggg gacgatcgct
    11161 cctatccacc cgggtatgtt cgacgaccaa gatcatgtta agcaggctgc tcacgacgct
    11221 gatgacgagg gcgccccaga aggacgcgcc gaatccaacc acggtaaaac ctttgacgag
    11281 ggcggcggtc agctgaagca acagggcgtt aatcaccacc atgaacaggc cgagcgagac
    11341 gacgatcagc gggagcgcca gcaggtagag cagcggccgg atgagcgtat tcagcaccgt
    11401 cagcacgagc acagcggcca gaccggcgcc gaggctctcc gattcgatgc cgggcacgat
    11461 ggtcacggcg agaaagacgg ccagtcccat gatcaacacc cgcatgacaa tgggacgcag
    11521 gccgccactg ctgcttgcgc tatgctgact cccctgcaac tgaaagactc gcatacggtt
    11581 ctctcaggat gttgaaaacg tcctccagct tcgttctcgc gtcattcaga ggctcaacgt
    11641 accgaagtgt acgcccgcct cttcgttcgc tgcggccgtg ctgaacggcc gttttgaaca
    11701 tcccctacca ttgccgccct ctccgtaacc ggcggctctt ggctatctat tgtgcctctt
    11761 tgggtttgga gtccgagtaa tgcacttcgg tagcggtcag gccgtcggtg cccttctcgg
    11821 cttcaatgac cacgcggtct cctacctgtg gcggactctt ggggcggcga aggttccgca
    11881 ccttgtattg cgtcttgtcc gtgattcgca ccgacacgac ctggcccttg ggggttttga
    11941 cctcgatgtg ggtcgcgtcg atggcggcca cgacgccgag cacatgctgt ccgctcccgt
    12001 gggcggccac cacggccggg gcgcagatca gaaggaacgc ggcaatgaag agtcgaagga
    12061 atgtcacggg tcgtcctttc gttgacgaat taatgatgat gcgagccggc ggccttggag
    12121 gctgcggcgt cgtccccgcc caggaactgg tcgaacaggg cttcttcctg gagttctttc
    12181 ttggtctttg gattcaagcc ggtcatctcc tgcaattctt cttcggtcag gctgggtaga
    12241 tgccggatga aatgcacgag ttgccagctg ccgctgtcct tggccggatc gccggtcccc
    12301 cagccgggca tggcggtgaa gcgtatgccg ttctgaataa tgaagaacaa ttctccgtcg
    12361 gacatggttt gcgtctccgg aagacgcatg tcaggcgctt tagggtagac gtttttcccg
    12421 atcacggtgt ccccgctgcc gttgttggcg tggcaggagg cgcaatggtc ggcgaaatgg
    12481 gcccgggcct ccttcatcag ctcctgcgtg acgggcaggg gattgcggag ttgccgattc
    12541 tcatacggaa tggccaggtg gcggagttgt ctggcgatca gcacctccag ctcgttcggt
    12601 tcggccttgg cgctaaagcc ggtgctatag gactgatacc cgaaccaccc gaggattccc
    12661 agggtggcga ggaagcccag catcagcagt ccacagattt ttttcatcgg ggacacacct
    12721 tccagttcag gcgcaacgcc atagaacaat atacagactc gattgaatga atgccaatga
    12781 tggcgcgttc gcagcggccc tgcttcgggt gtgtttacgc agattgtgtt gcaggcgcga
    12841 gcaggcgtag caacaggatg gccgtcaccg gcacatacat cagcaggccg gtggtgcctt
    12901 ggagtgtttc gccgatccag aacgtcatgg ccaggttaaa ggcgagcact ccgtaataga
    12961 gtccgccgcc cagcagcagg cgatgggtgg ttgaaggacg gtgatccggc aaggggtgtt
    13021 ggagccgtcg gtccaggggg aaatagatca agagggagat cgcgccgacg accgcccagc
    13081 ccacgtagtt cgccatcggg acgccgaaat gcacgccggg atccgggtag taataaatct
    13141 ttcccagaaa ccaacggtca cctcgcaacg cgaccggatc gatgaccata tcgatgaacg
    13201 cgaagagcag gatggttaag gcaaagaccg gccaacttgt ccggagcggc agatcgaact
    13261 tcagatcggg aagccgtgac gtagtgtcgg cgcatgaact cgattggacc ggcagcagca
    13321 ataatagagc caggcaataa ctggcgaaca gcaggaacga gaacgaaatc gagtccatga
    13381 aggggatgtt ggagaagtac agttcctgcc cgaccgtgga accgttgtaa aagtaccagc
    13441 cgaagggaat gccgttgcgc gtcgaagaaa attcacagac gaaggcggtg atccagctga
    13501 tgagccagaa gcgccaggtc cgcggccagc cgatcaacgc aatggctgaa accagaaacg
    13561 cggcgaggaa ggcaaaaacg taggggcgga gcagaatggt tttaaagaaa aggagaagga
    13621 tttccatcgg gggatcaacg gatgctcaaa aaaatcacgt tcttacccgt ccaccacggc
    13681 gcgtcaagac acggccctgc cccaggcgac gacaagaccg gaggcgtgcc agttcttacc
    13741 cacccacccc gagctgccgg gacagctctt tctccgtgga tatgttgagg agcctgtcga
    13801 gcggagaatg caggtggagg ccttttccag catccgacta agcgtagcca tgtgaacgaa
    13861 accatcgcac cgccttttca agcgccacct cgaccggtgt ttggggcagg ccgagttccc
    13921 ggatcgcttt actgcaatcg taatgcatct tgtatttggc catcttcacg ccttcgagcg
    13981 gaatgcgcgg gggcacaccc gtgaggttcg agatccactg attcgcatag gccaggggca
    14041 gaatggcgag gcgcggtagt ttgatggtcg gggccttcac cccggtgatc cggctgagga
    14101 tctggaacac gtcattgagg agcagattct tgttgccgag gatgtagcgt tcgccctgcc
    14161 ggcccttctg cattgcgagc aggtggcctt gggccacgtc gtccacgtcg atcaggttca
    14221 tgccggtttc gatgtaggcg ggcatgcggc ccttcatgaa gtccacgatg atttgaccgg
    14281 tgggggtggg cttcacatcg gcttccccga ccggcgcgct ggggttcacg atgacgactg
    14341 gtaagccttc gcgcgcgagt ttcagcactt cctgctcggc cagatatttt gaacgcttgt
    14401 aatggccggc catctgttcc agcgacaccg gcgtttcttc cgtccctggt cctccgcccg
    14461 gcggtaggcc gatcgcgccg atggtgctgc agtagacggt ccgttcgatg ccgacctcgc
    14521 gcgctgcctc cagcagggtg cgcgtgccgg tgacgttgat gtcgtagaag atcgaagggt
    14581 ctttcgccca aagggcgtaa tgcgccgcca cgtggtagag ctgccggcaa ccggtaaggg
    14641 cacgccggag cgactcgggg ttgcgtaggt ctccatcaac ctgttcgacc gggagcccgg
    14701 ccaggttctg gaggtctgcg cccttccgcg agagcacgcg aacctcgatg cctgtcttga
    14761 ccagggcgcg cgccacggcc ccgccgacaa aacccgttgc gccggtgacg agagctttca
    14821 tggggaagac gtaacaggtg aaacggcaaa ggtgaaaggt atgttgggag agatgatggt
    14881 ctggacgagg gggtcgcgtt tcacgtctta cgtttcacgc ctcacggtta attcttccgc
    14941 gaggtgatat accgggccag ttcgcggaga ggatcggctt tcgaaccgaa ggaggcgagc
    15001 cggtcgttgg ctcgggtgac gcactcttcc gccagttgct tggctccctc gacgccataa
    15061 aacgtcgggt aggttttttt gccgcgttgg gcgtcggtgt tgggattctt gccgagttcc
    15121 tcgcgcgtac cggtgacgtt taacacatcg tcggcgattt ggaaggccag gccgatttgc
    15181 tcggcataga aggtcaggtc gtcgagttgt gcgggcatcg ccccggcggt gatcgcgccc
    15241 atccgcacgg cggctcgaat cagcatgccg gttttgtgct tgtggatgga ctgcaacgtg
    15301 gcgaggtcga tgtccttgtt ttcggcctga atgtcgagga cttgcccgcc gaccatgccg
    15361 aggttgccgg acccgacggc cagttcgcgg atcagttgga cctggcgcgc ggcatcgaga
    15421 ctgtgctcgg cattcgcgcg ggcgcagagt tcaaaggcca tggtcaacag cgcgtcgccg
    15481 gccaggatcg ccatcgcttc gccgtagacc ttgtggttgg tcggtttccc gcggcgaaag
    15541 tcgtcattgt ccatcgccgg gagatcgtcg tgaatgaggg aataggtgtg gatcaattcc
    15601 agcgaggacg caacgggcaa aacagccttg ccaacgtggc cgaccgcttc ggcggcggcg
    15661 atggccagaa tgggccggac ccgcttcccg cccgcgaaca ggctgtaccg catgctctca
    15721 tgcagcgtcg tgggcatggt ctcggcactc ggaatgaccg attgcagata ctgatccacc
    15781 tcctcgcgtt tccgttcgag gtactgacgg atgtccagac cggtcggagt aagttggccc
    15841 gtggggctca tgccggagtg ctcagggggc tgtgcgtttt catgtgcgcg agagagtaac
    15901 aaaggggttc ggggagtgtc aaacggagga gaagagaggg ccagcgttcc gcatcctctt
    15961 gctcgcagaa cgcgcaccct aggaagtgcc tcgcttgggt gaatggcacg tctcggcgtg
    16021 ctagtgggtt gggcgggtaa gaaggttgcg cgcagttgag gattgctgga caccgccccc
    16081 tgtggcaata tggaaaaagg gtatccctca attgatgtaa gccggagaag tgttgcatgg
    16141 gtggaacgat aggcattgaa ttgccgaaac agcagattgc tgcgttttgt gagaaatggc
    16201 gcattcacca gctagccctt ttcggctccg tgctgcggag ggatttcagg ccggacagcg
    16261 atgtggacgt gttggtcacg tttgcgccgc aggccgactg gaaattcgag catttgcttg
    16321 aaatgaaggg tgaactcgaa tctctcatcg ggcggacggt ggatttggtc gaaaagcgcc
    16381 tgatcgacga aagtccgaat tacatacggc gcaaacatat tctgacccac atggaaccca
    16441 tctatgtggc gtgatgatgc gtatctgctc gatatgctca tcggggccag gcgcgtcctc
    16501 gaattccgcg ccgatcttac atgggagcag tttcgacaga gcggcctgca tcagcatgcg
    16561 attgcgaaag cgctcgaaaa catcggtgag gctgccggga agatttccga agaaactaga
    16621 gctcggttgt ctaccatacc gtggaagcag gtaattgcca tgcgtcatcg cattgcgcat
    16681 gactattttc ggctggatct gctaagggtc tgggagattg cagtgcatga cgttccggct
    16741 ttgatctcgg caatcgaacc ccatgttcct ccggaacacc cctgatatcg gttattctca
    16801 gcgatagctc ctctcacctc ttcgatcact cgactcagac tgaaatgggg ccggtgcccc
    16861 tcatccacct gctcgtgcac ctccgtatca gaagaccctc cttggattca gacgtcagga
    16921 tgattgggaa agccttgcca ggcagttgat ttccctcacg ggaaaggggt atccctcagc
    16981 ggctcaggct cagaggattg ttgcatgccc acgttgaatt ggatcggaaa agaagcggtg
    17041 gtgaatcacc accagcaggt gccgtttcat ctactgaagg atgtgcccga tctggcttgc
    17101 ggcgagccgg gcgagggcaa tctcatcgtg cagggcgaca acctcgtggc gctcaaggcg
    17161 ctcctaccct attacgcggg ccaggtgaag tgcatctaca tcgacccgcc gtacaacacg
    17221 ggcaacgaga actgggtcta caacgacaac gtgaacagtc ctctaatgcg cgaatggctc
    17281 ggcaaggttg tcggcaaaga gggtgagacg ctggaccggc acgaccgttg gctctgcatg
    17341 atgtatccga ggctcgcgct gttgcggcag tttctacggc aagatggtgt gattctaata
    17401 agcatcgatg atcatgaata tgaacgccta aagttgacaa tgaacgaaac ttttggtgct
    17461 gaaaatcacg ttgcaacatt catttggaag cggcggacgg ccacggacag ccgaaatcta
    17521 aatggtattt caggcgatca cgaatatatt ctgtgctacc aacgaactgc aggattccgt
    17581 gtgcaaggtc aagctaagga ccttggtaag tacacgaatt ccgacaatga ttcgcgcggt
    17641 ccttggatga gtgacaactt aaccgggtta gcgaatcctg ccgagcgccc caatcttcac
    17701 tatgaaatcg tcaatccgaa gacgggcata agctataagc ctcacccatc ccggggatgg
    17761 ctttacgggc gagagcgcat ggcagaactt atcgaagaca accgaatttt atggccacgg
    17821 aagcgggaag gacgtcccag gcttaagcgg ttcatatcgg atatgcaatc tgcaacgaca
    17881 gggttttcga cggtattaga cgcaccaggc aatgttgtgg caacgaagga actcgcagag
    17941 ataatggggc cgaaggcctt tgcctttccc aaaccgtcgc aacttattgg cctccttatc
    18001 gagcaggcaa caagccctgg tgacctaatt cttgactcct tcgctggttc cgggacgaca
    18061 ggacatgcca cattgacgtt gaacaacgtc gctcaggata cagcgccgcg ccgcttcatc
    18121 cttgtcgaga tggagccgaa gatcgccagc gacattactg ccgaacgggt acgtcgggtc
    18181 gcggagggct acaagaatgc caagggggag aaagtcgaag gcctcggcgg cgggttccgc
    18241 tactgcgagt taggagagcc gctgttcgat gagagcggca agatccgtga gactgtgagc
    18301 tttgccgatt tggcaaggca tgtgtatttc accgaaacgg gcgagccctt gccgcgtgga
    18361 cgcgtgacca aatcgccgtt tctcggcgag tgtcgtggcg tgggggtcta tctgctctat
    18421 aacggcattc tgaacgacaa gacggcgaat ggcggtaatg tgctcacacg ttctgtcttg
    18481 gcacagcttc cgaaattcga cgggcagaaa gtgatctatt gtgccggctg cctgttgggg
    18541 aaagaccggc tgcaggcgga gcggattctg atcaggcaga cgccctacga gatcaaggtg
    18601 agttgacgca tggctactcg caagaaatcg ccccctcctg gttccctgga gccgttgatt
    18661 cttgtcatca ggaatcagcg cgtgattctt gatgcagact tggctcgcct ctatggagta
    18721 acgacaaaac gattcaatga ggcattcaaa cggaaccgac agcggttccc gcatgatttc
    18781 gcctttcaac tgacgactac tgagttcggc aacttgaggt cgcaatttgc gacctcaagt
    18841 ttgcaacatg ctgaatcgac ggaggttcat ccgaggtggt cacagattgt gatcacctcc
    18901 cacggcgggc gtcggtattg cccctgggct ttcacggaac atggtgccgt gatggccgcg
    18961 aacattcttc gcagcgagcg ggcggtccag atgagtgtgt ttgtggtacg ggcatttgtc
    19021 cgcctgcgcg agcatgttgc cgcgaatcac gccattctga aacgtttggc cgaaatcgat
    19081 cgcacgttgc tcgagcacga cacggccttg ctggatgtct acgagaagct gttgccactg
    19141 ttgcagccgc ctccggatgc tcccacacgg cggattggct tccattcgaa gggcaagtcg
    19201 tgattacgct caaagactat caggagcggg tgttggagtc gttaggcgac ttcttccgct
    19261 gcactgccca gaccaaaacg ccggaggtcg ccttccggga ggtcacgcgt cgattcggtg
    19321 aggcagcgcc gtactttccg gtctcggctg cagggctcgg gccggacatg ccctatgtct
    19381 gcttgcgcgt gccgaccggg ggcggcaaga ccttactggc ctgctatgcg gctggattgg
    19441 ctcagcgcca gttcatgcgg gccgagcgag cggtggtgct ctggttggtg ccgagcaata
    19501 cgattctaga tcaaacggcg gatgcattgc gcgatccacg ccacccgtac cgccgtgcgc
    19561 tggaattggc ttgtggggcg gtcgaagtga tgaccatcga cgaggcattg cgtttgtcgc
    19621 gtgcagctgt cgacgggcaa acggtggtga tcgtgtccac cattcaatcg tttcgcgtcg
    19681 aggacacgac cgggcggagg gtctatgacc agaacggcgc gttttccgaa catgtgatga
    19741 atgtgcctcc tgaccgcctt ggggacctgt tccccggtgc ggacggaaag cccaaaccat
    19801 cgttggtcaa cgcgctgcgg cttcggcggc cgatcgtgat cgtggatgag gcgcacaatg
    19861 cccggaccga cctgtccttt gcgacgctcg gcaacgtcct gccctcgtgc atcgtcgagt
    19921 ttacggcgac gccggcacgc gaaaagactc cgtccaacgt gctgcatcgt gtctcggcgg
    19981 cggaattaaa aacggcgcag atggtcaagt tgccattgcg ggtggtcaca cgacacccga
    20041 gtcagcggga ccaactgctg gcggaagcct tcacgcttcg ggccgatctg gaaaggctcg
    20101 ccgccctgga agggcagcag acgaccgaat atatccgtcc cattcttttg attcaggctg
    20161 agcgggtcga tgcctgtgaa ccgctgcgtg agcggttggt gcgcgagttc gggctttcaa
    20221 acgacgaggt gaagatctcc gtcggcaggc tcgatgaact caaaggggtg aaggatatcg
    20281 cctcgccgaa gtgcccggtt cgggtgatca tcacggtgga gaaattgcgc gaggggtggg
    20341 attgcccctt tgcctatgtg ctctgcagct tgaaagaaac ctggtcggcc acggcgattg
    20401 agcaaattgt cgggcgcatc ctccggctgc cgaacgccaa ggcgaagcaa catcctgatc
    20461 tgaattgcgc ctatgccttc tctgtctcgg attcgattac agcggtattg gcggagctgc
    20521 gcgaggcgtt ggaacacaat gggtttacga aggcagaggc cgaacggatc attctttccg
    20581 tgccgcaagg cacgctgccg ctcggtgtgc aaccgcagac tgtgaccgtt ggtcctgacg
    20641 agatcgatcc cacagtcgtc caggtccagg agcccgcgct tggcggaaag gtgcggatcg
    20701 atgccgccag cggcgccatc accatagtcg tgccgctcga ccacgatgac ctggaaaagg
    20761 tgcaaagctg tgtcacgact ccgggcgcca aagccaggct cgcggaagcc gcggagatgg
    20821 tccgtcaggc ggagcaagcc ttcggcggaa ctggaaagcc gagaaagccc tcgccgtatg
    20881 agcagcagtt agactttctt gtgccgctgc tctgctttgc cgagaacggc atgctgtacg
    20941 agttcgaaag cacctttttg ctcgaccacc cctggaagct gagcgagaaa gatgcgtcgt
    21001 tgcctgcggc gtataacccg ctggctcgtc cgtatgggaa ggttggggtg atcgatgtcg
    21061 gacagaaggg cgacgtccag actactctgc tgggcgatac aggcgatgcc gattttgtcg
    21121 gaacgctgca ccagcagatg tttcacttca gcgggcaaga tgactggtcc gtagaaaggc
    21181 tcgtcgcctg gctggatcgg gagatcgatc atcacgacat tcccgtcggg gaatcggcag
    21241 agtttcttcg aaaggtcatt cgtgggctga tggcgaagta tgagattgcg gacatcggta
    21301 ctctggcgct cgatcgattc cggctccgcg atgaaattgc ggcgcgtatt caggaccatc
    21361 gagaaggtga gcggaaggcg tcatttcaac tgctgctcct atccgactca ccgctcactg
    21421 tgaccgacga gcgaacgatt aacttcaaga ccattggcta tgagccgagc cgtgtgtatg
    21481 agggcggctt tcagtttcag aagcattact ttggatccag gcccggtgag ttgacggaaa
    21541 agacggccga agggcggatc acagaagagt ttcaatgtgc gcagtttctc gatggactgc
    21601 cgcaggtgag attctgggtg agaaatcttg cacgcaaatc gacttccttc cggctgcaaa
    21661 catccaagga ctggttctat cctgattttc tgtgtcaact gacggacggc cgtacgctgg
    21721 cggtcgaata taagggaaaa catctcttcg acggtgtcga tgccgaagat aaacgagcgg
    21781 ttggagagat atgggcctcc cggagcgatg gccgctgtct cttcgtgatg ccgaccgacg
    21841 gcgatttttc aacgattcgt aagatgctgg ccgcataacc cctgatcacg ataccggtct
    21901 caggaggggc atgggttgca gagaattcca gcaggttgta gagttttcca aaacagcgta
    21961 gggtttctga gacatttttc aaggagtgga gccgatgacg acccttgcct tgactattga
    22021 aaaacaggcc cggaagcttt cgccgactga gcgtgaacgg ttggctgagc gactcatggc
    22081 agggatgacc ggcgaacgcc tgacctcagt ggacgaagcc tgggtcgcag aggcagaaca
    22141 acgattttcc gcctggaagc gaaaacttac caagccgctt tcggtcgcca agtcgttgcg
    22201 cgacatccgc aaggatctcc gtcgttgagg ctgcttcttg atccggcagc taaagccgaa
    22261 ttgagccagg cagccctcta ctatgaggat tgccgccacg gcctgggcga agagtttttg
    22321 gttgccgttg aggcggcatt cgaccaaatc cggaaatatc ccactctttg gcgaactctc
    22381 aagggaagat tccggcgtta tctggtccag caattcccct atggcgtgat ctatgccgtc
    22441 gaagggcgga caatctatgt ggctgcggtg atgcacgtga agcgcaagcc aggttactgg
    22501 gcatcccgaa acaagctttc atgagtcggg gggctccttg ttttttcttg gaccccttcg
    22561 ctatacttcc gcgctatgag tattcgtaaa gccgccggtg agtgggaacc tatgctgctg
    22621 gggatcgagc cccgcaactg caaggtctgt cagcagggtc tctaccgtga taagacgatg
    22681 ttgcgcggcg gctggtcgcg ttgcgcggtg tgcgatgagt ttgtgcacta tagttgtctt
    22741 gccagcggaa agatcagctt cctcaagcag cgtccgcgca tctgcaaaac ctgccggacc
    22801 gccgaggaac aggcgaagca agccgttccg ccgccttcca gccaacccgc accagtcggg
    22861 tcatgaccct cttactcgat ccgcagcaac gatccaccag acctgtctgg cacgtctact
    22921 tttcccacac ccttcgctat gtgcttgcgc cgcttctgtt tatttccagc ggcttcttta
    22981 cggccaattt catcgtgagc gggcactaca cctggcctcg cacgagtcgg gtcttcgtgc
    23041 tgaccctcac gctgctgatt ctcgcctacg agttcattta caaagatcat accgttcgat
    23101 cgacttcgcc ggagcgagcc ttcaacgccg tgctgtattc ctgtatgatc ccctacgccc
    23161 tcggggtgct catgatgctg gggttggcaa aactgtagcg gcatggtgaa gcgtgccacg
    23221 tgaaacgtat ttcgtggggg taagagcgta tggctgatgg catagagctg acagcaagta
    23281 cgcccgctct cggtttcttg ccttccgcta taagctatac gccataggct cttttggctc
    23341 ttctacaggt ggttatgggg caacgatttc aaagcggcgc cttcgtgcaa cagtgtttcg
    23401 ccgtccatcc cttgtgcctg tcgttgaagc gaatcgacga gacggggcgg ctgatcatcg
    23461 cctgcacgtc ctgtcgcatg atgcatcaca tggcagcgga ccaggtggtg gcgcgggtgg
    23521 cggctgtgcc ggtagacggc gacatgtcgg gtgccaaggc gctcccgacg gcgaaggaac
    23581 tgctagaggc ctgccttcag gcgcatcccg tgtccgtcac gattcgcgaa ttggatgtga
    23641 tccgcgatag cgtaggcctg cgttgcgccg actgtcgccg gatattcgat ttgcaggtgt
    23701 cggtgttcga atcccaccag aagtcctagt cggatgctga aaaagtccgc tggcatctcg
    23761 cgcaacagcc gtgaaacgtg aaatgtaaaa cgtgaaacgt accttgtcgg acccgacccg
    23821 tctcggatca cgaacgatgg cttctccgtc acaagactcc tcgctgacac ctgctgaact
    23881 taccttggtt gcggacgggg agctgtttcg tgccaaggag cgtatcatcc ttaaggtgcg
    23941 cgatctgctc actgaactcc aggccgggtt gcaggaagac ctgagccgga ccgcgctgct
    24001 ggtgccgccc gggttcgatc catccaaaat gcaggttgtg aagggcgagc gcctcgaaga
    24061 gtgcccctat caatacctgg attaccccaa acatttcctc ggggacgaca agttcacctt
    24121 tcgctcgctc tgctggtggg gccaccatct ggtctttgcc ataattgtgg aaggggggca
    24181 ggccaaacag tgcaagaaga acttctttga tcgattccac cagctggccg gtcaggggct
    24241 ggagctgtcc ctcgctccca cgctctggga atggaagcgg ggcgaaggct acaccctgcc
    24301 gatcacccat gaccgcaagg ctcaattggc ggccgtcttg tccggtcggg cctggttcaa
    24361 gattgcccgg ttcgtaccgt tggatcagcc ggctatgtcc gaaggccgac tgtcggaggt
    24421 gggccgtgag actttccgga gcctcctgcc gctcctgacg ccttgatcgg ccctgcattc
    24481 gacttgttcc agccgtgcgt gttctccgtc atcggcattc agcgcgccgc gccgacgcgt
    24541 ttcttccaga cctccttccc gtttgtcatc cggctgcgag ttgggtagac tgtgcgcgtc
    24601 tttggggaac gtcacttact gacagggagg catgtgtgat tgcacaggga tgtcgcatgg
    24661 tgggtcagtg ttgtgtcgca gtgggcctgg cgttgctgct gggctgtgcc ggcacgggag
    24721 aaatggtctc gctgaatctt cagacgcaac agccgttcgc gcagagcagc cagactgagc
    24781 cgttgaagat tgtgatcgaa ccatttgaag attcgcgaac cgaccggtcg aagatcggcc
    24841 aacgtaccca tttgggcggt ggggtcacga atttcaacgt caacggaggc gcgccgggag
    24901 tgacgattgc cgaggcgctg gccgaaacgc tgcgccagcg gggatggaac cgccgcggtt
    24961 gggacgcgcg cgtcgtacag gcgggcgtcg gagtgtcagg cgcggatatc gtgatcgggg
    25021 gagaaatccg tgacttctcg acgaacgcga agagccgggt cttcaatacc aaattgaccg
    25081 gcgagagccg gttggtcatc aaggccagga atctcgcgga cgacagcagc actgtgcgca
    25141 acattcaggg cgaacagacc aagctggtgt tctggttcac cagcgaagat gtcgaaaccc
    25201 tcatgtccgg cctcctgcaa gacgggattg agcggtttat caccgatacc aaaatcgagg
    25261 gccgcacgct gaagtcagcc aaatgaagcg gtccggtttc ggccgtcgcg tggcgagtgc
    25321 ggagggtggt tgtgcgtgaa cggcggtggg agacctcggg ccgattgacc tttcgcgagg
    25381 tgctggcggt cggcgagcgg ctggcgactc tgggactgaa gcccgcgcat cccgccaagg
    25441 atgtgatttg ctacgtggag gattgggcgg tcgattctcc cgacgcgttc gatcaactcg
    25501 atcactgggc gacggaggac gtcacgcttc tgcatatccg cgaggcgtgg caaggcgatt
    25561 tttttctact cgcaggcggc taccacacgg tttaccagcg gtttcaggac gtcggtacct
    25621 actgctccat cagccatccc tggcgcacga aacccgggct gcggtttcat catcccacgt
    25681 gcatgttctg gctgggcttc cggcataacc atgcctttat ccgcgtgcgc ctgcataccc
    25741 aggaggtggt gacgcccggg gaaacgcgtg aggacgcgcg gcgggtggaa tggatcgatg
    25801 aacggcgggc gatctttctc gacgccatcg ggacgttgga cctgccggtg gaaacttccg
    25861 tggagaaggg cggcctggtg ttgaggccgg cggatccggc gacgccgttt ttctgttcct
    25921 ggccggatgc cttcggcccc tgtcagttcg aatacaactc cttggatgcc tacgagtttc
    25981 tcgtgccggc gagccggttg gcggccacct ttgcgccgca gccggccggt gtgcgcgtct
    26041 acctgacggg tttttcggag ccggcgctgg acgcatttgc ggcggtacag ctaggcgccc
    26101 gttcggtgta tcgctgttcc gtgcattgcg cactgggcga attgccggaa gtgctcgcca
    26161 cgatcgagcc gcagggacgc ctctatagca cgctgtgcga atttcaaaca caggagttga
    26221 tgccggaagg ggacgatgcg tcggccatta tcgggatcgt cggcaccgcc ggtggattcc
    26281 agttggaggt ccgtttgaat cgcgctccct tgcctgaaga acatatggct gcgtggctgg
    26341 aacgtctgtt gggatatgat gtgacgtatg cgccgctccc acccttcatg taatccggca
    26401 gctgaacatg gccctcaact tcgttctcgt ttcgaaagaa tcctcaacgt accgcagagg
    26461 gtacacctcc ggttctttct cccctgcggc ctcgttggat gaccatgttg agctgccggt
    26521 tgtcgttgat caaagcgggt gctcgtgacc cgtcattgaa ttgatccact cgctgattga
    26581 tgcgaccaat tcctctcttc gtttgacaat cgtccgcttc tcctgaaatc tagcccatgg
    26641 tctcgatgca acgcttccaa tcggtgttgg ggaagcccta ccttccgccg ctgtttttct
    26701 tcagtggcgt gacctatgac acggtcacgc tgacccgtat cgaccgcctg caggacaatc
    26761 tgttgctgct gctctatctc accctgttgg gctttctcat tgtgctgacc ggccggttgg
    26821 gcacgaatga ggcgacggtc gaggatctcc cgccggatgc gccacggctc atgagatgga
    26881 tcgtgcaggc caagccctat gcgccgatgg cgattcagtt tctgctgggc agcctgttca
    26941 gcgcctacgc gattttttat tccaagagcg cgacctttac ggggaccgcg gtctttttct
    27001 gcctgttggt gggattcctg gtcgccaacg aattcctacg cgaccggctc tcgaacgtgc
    27061 agttgctagt cggcctctat gccctggtct gcttcggatt cttcacgttt ttcctgcccg
    27121 tcatgaccgg cttgatgaac caggccattt ttcttgccgg ggccgcgctg accgtgttgg
    27181 tcgtcctgcg cgtcgtgcag ctgatctatt ggcgaaaccc ggctcgcacc aatcgcgagg
    27241 cgctgctggc cggcgcgccg gcgatggggc tgatcggcct gttggtcggg ttctactttc
    27301 tgaactggat tccgccggtg ccgctctcga tgaagttcgg ggggatgtac cacgaggtca
    27361 agcgcgccgg cgaccatttc gaactgtcgt atgagaagcg gtggtttcag gtgtggcagc
    27421 gatcgaatac gaccgtggcc gccgacgaac cgatctattg cttcaccgcg gtgtttgcgc
    27481 cagtgacgct gaagaccacg gtctatcacc actggtacta ccgcagcgac aagagcaagc
    27541 ccttcagcca tgccgacaag attcccctca aaatcgccgg ggggcgtgaa ggcgggtatc
    27601 gcgcctatac gtttaagcag ggattggatg tcggtgactg gcgggtggat gtggaaaccg
    27661 aagacgggcg tgtgatcggc cgggtgccgg tgcacgtaga gaaacgggac gatgcatcgc
    27721 ggtcgttgac gacgattgtg tactgaccgg caactggagt cgcacatgcg ccggatcaag
    27781 gggctggaac tcctcgataa acaggaaagg ttgacgcttg ggctcaatat tcagacggct
    27841 tcaacaagtt ttgaatgggc atgtgcggcg aatacgctaa gatgatcgag tcgtgatctt
    27901 aaaaatctcg gcagcggtga tgtgtttatc ttggttttcc gcacgccatg ataaagaaca
    27961 ggcggcatag ggaggtccgg ttatggtcag gtatcggctg aggctcatgg cggttattgt
    28021 tctggtactg gctgcttggg cgctgcccgc catcgcagag gagccgccgc agcaggcgct
    28081 tgcgtggcag gtcggacctg gtgaggccaa gttaggagat caggccgtcc tgaagctgcc
    28141 gaaggggtat cggtttctcg ggggtcagga aacccaacgc ctgctcaagc agatggggaa
    28201 tttcccctcc ggtgccgagc tgggcctgat taccgcgaca gcagaaaatg agcagtggtt
    28261 catggtggtc cggtacatcg atgcgggtta tgtgaaagac gatgaggcgg ccaattggga
    28321 cgccgatgcg ctgatgacct ccatcaaaga gggaacggac gaggataaca agactcgcca
    28381 ggcgcagggt tttcccccgc tggtcattcg tggttgggag gaaaagccgc attacgataa
    28441 ggcggcgagc aaggtggtct gggccatctc ggcgcaggaa cgggaaaccg tgggggtgaa
    28501 ttacaacacc ctggcactgg ggcggcaagg gtacctcagc atgaacatgg tcgggtccct
    28561 ggagcagttg ccggtgctga agccgcacgt cggccttctg ttgtccaatg tggagtttat
    28621 cgaggggaaa cggtataccg atttcgatag tacgaccgac aaggtggccg cggtcggcct
    28681 gtcggcgctc atcgccggcg cggcgattaa gtccggcctg ctggccaagc tctgggcttt
    28741 tattattccc ctggtgatcg caggaaagaa gttgttgatg ctgctggtga tcgcgctcgg
    28801 cgggttggcc gccaagtatt tcaacaagaa acccaagcca gagcaggcgg gcggggacgg
    28861 agggctgtcg tcgtgaaaat tctcctcctg ctcctcggtg gtctcaagct gggcaagatt
    28921 gcgcttaccg gcgggaccat gctgctgtcg atggtggtct atagtttcat cttcggctgg
    28981 tggtatgcgg tggggttcgt gctgttgatc ctgttccacg agctggggca ctacgctgcc
    29041 gccaagcagc gcaacctgaa cgtcggcgca ccgacgttca ttcccttcgt cggggcctgg
    29101 attcagctga aggaacagcc gatggatgtc gaaaccgagg cctatgtcgg catcgccggt
    29161 ccggtggcgg gaacctttgc cgcgatggcc tgctactatg tggcggagta tacgcacagt
    29221 cagttgatgc tggccctggc ctatgccggg ttcatgatca atctgttcaa cctcattccc
    29281 ctctcaccgc tggatggcgg gcgcatcaca gccatcatct cgccgaaggt ctggtggctg
    29341 ggcgtgccga tcctcatcgg gctctttgtc atgaaccaca gccccatgct cttattgatt
    29401 gcgatcatgg ccattcccca tctgatggcc accttccggg gcggccccca cggcctgccg
    29461 gaacggtact acgatgtgcc gctctcaacc agggtgagtt acggcctgta ctacctcggg
    29521 ctggcggcgt ttcttggcat catgagttat gagacgcatc tcctgctccc ttcccgcaat
    29581 taaccgacag caccacgagt taggtctctg tgcacacgtt cagcgctgaa gtgagtcgta
    29641 ttcaggacct cacgcacgac gtgcgcgcga tcgagttgcg cctcctggag ccatccagca
    29701 tcgcgtttaa ggccgggcag ttcgtgtcat ttgaagtgcc gaaggaaggg cagcctcgac
    29761 cggtaaccag gccctattcc atcgcttcgc cgccgggtca gcgggaccgg attctgctcg
    29821 tgctgaatct cgttcagggt ggaccggggt cgagttatct gttcggctta cgggaaggag
    29881 agcgagcttc attcaagggg ccggccggtg ccttctatct gcgggacgag ggggtgagag
    29941 acctgttgtt cgtcgcgacg ggaaccggga ttgctccgct ccgcagcatg atcctggcgc
    30001 aattggagcg tggggagtcg cgatccgtca ctctgttctg gggactccgc agtcagcgag
    30061 ttctctattg gcaggaggaa ctgacggcgc tggcggcgga ccatcccaat ttttcttttg
    30121 tgacgacact gtccaggccg gacccggggt gggaaggggc gagcgggcga gtgacggcgt
    30181 tgatcgatgc gcaggtctcc tccgtcggga atctcgccgt ctatctgtgt ggggggagcg
    30241 ggatgatcaa ggacgtgacg gccaggctca atgccaaagg gctgtgtcct atctatcgcg
    30301 agaagtatta cgactagcag gccgatgaaa gagggcgcca acggcgttct cgcttcgctc
    30361 actcctttga cataccggaa cagtatgcct cagtcgttcg ctcgctgcgg ccttgttcgc
    30421 gacccctttg atcagcctgg aggcctctcc ccttccgagg caggtgacct actcagtctt
    30481 gggggcgcgc ttggtggtat gctgaaacac gcacttgggg caggtatagt gaatgaactg
    30541 gccgcctgcg accagacgct tggtcatggg gaccttgcac caggtgcaga gacgttccgg
    30601 caattcagcg gtcggtgcgg aaggattgga ttggggtgtc gacgtatcag ccatggtcgg
    30661 cattctcgct aaggggtcga gagtttgtca atgtgcggga agcacgagaa atttcggaga
    30721 taaatattta cagggtttca aacgttcagt atattgcaaa catgtgttcc cactcccacc
    30781 attcccaaca gaaaggtcgt atcgcatatg cacaacgcag aaggtgccac ctccgacgac
    30841 atctcatcat cttcagccag ggttgctgac tccgcagtcc atccggactc tgtcggaccc
    30901 ggcaaagcct ggggcatttg cacctccgtt gacctccacg attgcatccc cgaacgcatt
    30961 cgcgacgcca aacaaatcga agcctacgtt gtgcagctct gcgaactgat cgaaatgaag
    31021 cggtatggtg cctgccagat cgtgaatttc ggagaaggcc gcgtggcggg ctacagcatg
    31081 gtgcagttga tcgagacctc cttgatcagc ggccacttcg ccaacgatac caacagcgcc
    31141 tatctcgaca ttttcagttg caagggctat gagccggccg tcgtcgaaga attctccaag
    31201 gccttcttcg gcgcccgtcg ttcgagccat caggccatgt tgcggtacta ggccaagggc
    31261 acagatactt tcggtttccg gcaggaggcg ttgtcttgcc ggaactatat ccgatgaatc
    31321 cgacaccacc caaaaccgtg cgggcgtttt tctccacact cgccggtaaa ctggatcctg
    31381 aagcagccga aggactggat gcggtctacc agttcaacct gaacggcgcc gatggaggac
    31441 agtaccaact gcagatccgg gacggggcct gtcaggtttc cgaaggcacc cacccggatc
    31501 cgcatgtgac cctggcgatg tcgggggagg attgtctgaa ggtgttgaac ggtcaggtca
    31561 gcggcaccat ggtggccatg accgggcggc tgcgtatcag tggtgatatg ggcctggcca
    31621 tgcagctggc ctctctcttt cccagtctcc gcccctaaga gtctctccaa ctctgcggca
    31681 aacgaactgc cggcatcggc gtggtttttg gccccaaaaa atcctcaacg tattccagcg
    31741 aatacgcctc cggttttttc gtgcctgcgg cctcgcatct accaacaact cctgtgcctc
    31801 gcaacgaagg atgactcgga cagactctta gcagactctt cagaagaacc gccagcggtg
    31861 ttctcggtcg cacgcctcca tacgaagtgc aaatggtaaa acggaactac tggtggggtg
    31921 tatccgttcg ccaggaatat ctaaagcggc gaacgggtca aacgaagttt ggtttgtacc
    31981 gcctcagtcg tcgtgcttcc tgcggccttg ctggacggcc tttctgaaaa ccctgcgcat
    32041 cgtaccgtgc ctcagagcgg tctctggtac gaagaccttt tggagtacag gtaaagtttg
    32101 gtcaaggggg cgaagcatga acctcttgcc caactatggt ctccgctttc ccctggagtc
    32161 taaccaagac agtttgcgcg tatgtcaaaa aatcgctcag ggctccaatg agattagctc
    32221 ccttcaacgg aggggattca ttaaaagcta cgtacatatg gagtgtgtac ttatagtccg
    32281 tttctatgct gctggggaat ctggcaagga cgatctcgtt tttcatcgta tcccagcgcg
    32341 aattgcccaa ctcgaatttt gccggtccct tcatgctgcc agagaaactg gggaaatcaa
    32401 tgccggaaac tgcggcacca aaattcaatc tgatcgtatg cttccgatta gctatcttgg
    32461 ccaattccct aataaagtta tggcctccag cgtaggcatc agatctgccg tatggctcca
    32521 gctcgcgcaa gacatcccac aactctattg ggactctaat cttgggcgtc ttcccaggct
    32581 tggccttggc gcccaattta taatcaaggt ctgtgggact atcggcccag gggaagtaaa
    32641 tggagtcctt ggggggatgg ccgatggccg tacacgcggc ataaatggaa cggtcaaaag
    32701 agtgccgtat gttcagcaac gcctcgtttg cctttctggg gatgctttgc ggcagtttcg
    32761 agacaagcac gatcttcaag acgttttggt tgctatcggc atcgaactca cggacttctc
    32821 gaaaattgtt cgcattgaaa aacgcatcac aactgatctt gagttcatga atagcgtccc
    32881 ttgcccaatc gagcatttct atggcgctgg cgaaatgatg agatgacata gtccggtcgc
    32941 tcatcctata agagcccgac gatctcttct aatgaccaca catgatcggt aacgcccgcc
    33001 tgcatggctg gcgtaactcg cagagtttga tggatgcgag cgaagttgta gtacatgaaa
    33061 tagagcgcca atgcatgatg gtgattctcg accttcttgc tgtgtgcatt ggtcagtatg
    33121 gtgaacctcc gccttcccat ccgaatctgc aagttcatcc gctctgcata gctggttgag
    33181 atatgcgcat cttcaggctt gcccatgatt ttgtgcgttt tggtcccgca gcattgtgcg
    33241 gggctgtagc gagtcgtgac gccttcttgt ggcgggactg cataaatctt ctcaagcatc
    33301 gcgtagtcaa tatccctttt ttaggcctgc tgactcacta actcactctg aacccgctct
    33361 tctcccagct gatccgcccg acgtggaaag cgctcttcta acatggtata gacggtcggc
    33421 acgaggaaca gggtcaggat cgtcgagacg gtgagccctc ccacgacggc gcgcgcgagc
    33481 ggggcattgg tctcgctgcc ggtgccgagc ccgagggcca tgggcatcag ccctaccact
    33541 gtagccagcg aggtcatcag aatagggcgg agtctggtgc gcgacgccgt gacgacggcg
    33601 gtggagaggg ccaggccgcg gcgtcgcagc acattggtgt agtccaccag caggacgccg
    33661 ttggagacga cgatgcccag catcatgatg atgcccatca gcgacgacgt ggagatcgtg
    33721 gtgttggtca gatagaggat cacgatcacg ccggggattc ccatcggcac ggaaaacatg
    33781 atgatgaagg ggtcgatcaa cgatttgaat tgtgcggcca tcaccatgta gaccaggatc
    33841 aatgccagga cgctggcata ggtgaggcct tcgaacgtct cgcgttgttg ttggatttgt
    33901 ccggcgagcc ggatgctgaa tcccggcggc aactgcatct gggcgaacgc cgactccagg
    33961 tcctgggcaa tcgagccgag gtcgcggccg acagggttgg cggtaatgtg gacgacacgc
    34021 tggaagtatt tgcgctcgat cttcacggga ccggcgttca gtttcagggt ggccaggttc
    34081 ttgaggagaa ccggctctcc gtttttcgtc gtcagcagga catgctcgat ggccgacagg
    34141 tccttgcgat gttctttcgc gagccaggcg ctgatgtagt attcattgcc gttctggggg
    34201 tcggtgaaga tgatcggatc ggtctggccg tttccgttca gtgaaaacag gacggcgttg
    34261 gccacgtccg tttcgctgat ccccagcagc gcggcttttt cgcgatcgac gaccacgttg
    34321 acctcagggt agttttcctc ccggctgact tcgatgtcgg ccatgcccgg aatcttatgc
    34381 atgaggtcct gcacctggtg gatgacggtg cgggcctttt cgaagtcgta cccgtaaatt
    34441 tcgacatcga tggatttctg cgaaccgaaa ctggtgaccc gtttgaccag gcctcccgga
    34501 tcgaagaaca tcgcgacgcc ggggaagagc ttgaggatcg cgggtctcac cgcattcatg
    34561 atttcgacct ggttccgccg tcgcttgtcg ggggacgaca aatagatgga aatgaccgag
    34621 gtgtgcgggc cggtgttggg attgaacagg gacgaacgcc cctgcgccag gaccccggtg
    34681 ctggaggcga tcgcttccag ttcatccggc ggaatctttt cccgtagcac acgctcgact
    34741 tcggcgacct gatcgacggt tttttccacc cgctgaccga ccggcccgcg aagcacgatc
    34801 cggaactggc tttcgtccga gaccggaaga aactcggtcc cgatgaaggg cagcagcgca
    34861 agcgatccga cgaagatcgt gaccactgaa accagcaacg tgcgccggtg ggccagcacc
    34921 cagcgcaggc tgcgttcgta tccttcatcg agcgcgtcgt accggcgctg gctccactgc
    34981 atgatgctca cgaaccagga gggcaaggaa cggtgtgcct cctgttccga cttcaagaag
    35041 cgataacaga gcgcgggggt caccgtgcgg gagacgaaga acgaggtgaa cagggcgatg
    35101 gcgatcgtga gggtcaacgg aatgagcagg agccgcgcga tcccgacgat gaaaaacatg
    35161 ggcaggaaga cgacgacggt ggtgaccgtg gaggcgaaga tcggcatggc gacttcgcgg
    35221 gcggcgttga cgatggcgtc ccaccggttc cggtcgacgt tcaaatggcg ctgaatgttc
    35281 tccaattcca cgatggaatc gtccactaaa cgaccgatgc ctagggccag gcctcccatg
    35341 gtgaagacgt tcagggtctg gttggtaaag tacagcacga taaaggtcac gagaatcgac
    35401 agcggaatgg cgacggagat gatcagcgtg ctggtcaggt tcctcagaaa cagcagaatg
    35461 accgccgccg cgagcagcga accatggagg gcctgctcga tgaggttctt gatcgattgc
    35521 cggatataga tcgattggtc gaaggagatg cccaattgca cgcccggcgg aatgccgatc
    35581 attttgggga tcgccttccg caaggcatcg acgacttcca ccgtgttcgc aatcggttgc
    35641 ttgttcaccc gcaggtagac ggagcgtttg ccgtccgtgc ggaccacgtt ggtttgaatg
    35701 tccgacgagt cgctgagcac gccgacatcg cgcacgcgca ccgggttacc ccgttggtcg
    35761 attttcacga cgacgtcgtt gatgggctcc accgtcttga actgcgtgtt ggtgaacacg
    35821 ttgtagtcga ggttcccggc tttgatatcg ccggacggca aaatcaggtt ggaggccttc
    35881 acggcgctga ccacatccag aatggagagg ccgcgcgcct ggagcagggc cgggtcgaga
    35941 ttgatattga tctgccggat cttgccgcct tcgaccgtgg ccgccgccac gttggagatc
    36001 tgttcgatct gcggggcgat cgtgttgtag gccaggtcgt agagggctcg ctcatccagg
    36061 tcgccaccgg aggccgtcac gaaggcgacg ggaatgttcg agacatcgaa cttgacgatg
    36121 aacggttgca gaatgccggg cggcaggctg ttcagaatct gggtgatgcg ctgcatcacc
    36181 tccatctggc cgacgttgat gtccgcgccc cagttgaacc agacctgcac cgcgccgatg
    36241 ccttgtttgg cgaaggactc gacatgttcg acgttcgagg ccgaactgac ggccttctcg
    36301 atggggtaga ccacgctctg ttcgatatcg agcggaggcg cacccttata gatgacgccg
    36361 acaaacgcga ccggaacttg aatgttgggg aagagatcga ccgggagccg ttcgagggag
    36421 gtggccccca ggatcaccat ggccagggac agcatcagga tgccgatgcg attgcgcaat
    36481 gcgagcagtg tcagccacat gattgagacg tgagcggtga aacgttagat gtgaaacgaa
    36541 gtgagcacac cgtcaacgca ggaggtccct tcacatgtta ccttttacga ctcacgtttc
    36601 acggtatcca tcggctgcgt ttgaaccgcc gcgccttcgc tcaccaggtc tttgccggag
    36661 acaatcacct gttcgtctcc cgtgaggccc ttcaccactt cgacgcgatt ctccgcgcgg
    36721 gctcccagct caaccggcac ctgatgggcc ttcccgtcct tgacgacata gacgtactgt
    36781 gactcttcga gccggctcac cgcatccagg ggaatctgaa tcgcctgggg atgttttcct
    36841 accaggacct cgacgcgggc gaacatgccg cctttcagac gatgatcctt attcgggagg
    36901 tcgacctcga cggtcatagt gcgggtggcg cgattgaggg cctgcacgat gcgggtcacc
    36961 gtgccttcaa agacttcgtt cgggtaggct tcggcgcgca catccgcgcg ctgcccgacc
    37021 ttcaccagcg gcacatcctt ctccacgact tcgatcaggg tgcggacggt ttcgacgtca
    37081 tgcacactca aaataccgcg cgacatcgtg gacgtgctgg cggtggtgcc gctcacatac
    37141 gcgccgggat ccagattacg ctccgcgata tagccggcaa acggggcgcg gatggaggcg
    37201 taggccagat tggtgacggc ctgggctaac gcgacatcca tctgttgcac ctgtgcgcgc
    37261 aaggagtcct gcaaggccag cgccgcgtcg cggttcacca gcgcggtatc cagatcctgc
    37321 tgtgagacaa actggtcctt gatgagggcc tgcatgcggt cgagcgtgag ggcggcattg
    37381 cgcacggccg cctcctgctg cacgaccttc gccttggccg acaggagatt ggccttcgcc
    37441 tgattcaccg cgtgcacata gtcggtgtga tcgatttcga cgagcagttg attggtcttc
    37501 accaagtcgc ctttatccac atagatcttg gcgatgtagc cgtccacgcg cgagaagata
    37561 ttcaccagct ggttggggat gaggtcggcg gtgtaggtta atcgtacgtc caggtcctgc
    37621 ttgatggggc tcatggtgcc gacggtgagg atccggtttt tccggggatc gctcttggct
    37681 ccgctgctca gacggagcgc gaccagcgcc accacggcca caaagagcat gatgccgagg
    37741 gtcacgatcg ggtgccgtct gatgagatta acggacacgc gacagccccc gcttcctggg
    37801 tgtggacgaa cgccgcaatc cgttcaggaa cagctccacg taggtggaga cgatctcctt
    37861 gtgcgggcaa tgcaacggca cgtcgaaaac ttcgtgcaac aggcggtggt gcacgaccat
    37921 cccgatgaag gcgcgcgcgg ccaacagcgg atcgatctcg cggaacgcgc cttcttcaat
    37981 cctggtccga atgtatcccg ccaggtagtc atagaagacg cggtgctgct tgccgaaaaa
    38041 catgtcggcc agttcgtggc cctccaacgc gctgaagagc aggagtcgca gcatggtggg
    38101 gtcggcgccg ggacggatgc ggtacccggc gatgagtgtg aagacccgtt gatcgtcgcg
    38161 cttgcgggcc acttcctcga cggcgtgtag gagctcgctg agcggggcct tttcggcgag
    38221 gatcgcagtg tacagcgccc gcttggtcgg gaaatatttg aacaccaggg cttcgctgac
    38281 gccggcggcg cgcgcgatgg ccttggtagt cgtgcccttg aagcccttgg ccgcaaacag
    38341 actggcggcg gaggagatca ggctggcctg gcggtcttgg cccggattgc gggatagggc
    38401 gttcgtcggc ttcatacgat cggcaatcag gtgagtgagt ggttactcac ctgtcaggct
    38461 atcacgggcc aactcggcaa gacaagaatc tcgctggttt cgggacgacg tgttcggcca
    38521 tggaggaagg gaacggtctt aaaacgtcga aggcgaattg ccctcgttga acttagcacg
    38581 aaaggcttcg ctctcgaaga agatgacgcc ctggctggtg cgcaggtcat agcggagaat
    38641 gatttcgctt tcgtcaccct gccagttgaa aaacttcacg gcgccgcccg cgacctggcc
    38701 gggagtctgg tcgagcgggc cgaatcgttc ctgcaggtaa ctcagtatcc ggtcgtgggc
    38761 ctctttgccg ctgtatcgca ccatgactcg cgcgaatttc cgatcgacgg tgctgaactt
    38821 catcgaatcg accgtcgccg accccaatgt ccggtcggcg gatttcagtt cataggtctg
    38881 gacccggcct gtgtcttcga ccttcacgaa cgtgtcggat tcggagaacg acgcacccca
    38941 gggaatgcct tcgaacccct tcgggtcgtt ctgcatgggg acggccagcg ccagcgcaac
    39001 cggaagcagc agcgcggcaa cgaacgcagt gagggccgtg ggccattgtc caggagtgat
    39061 gaagaggagc tgagccatgt tattccgccg tatccgacat ccggtcctga aatctcggcg
    39121 cgaggttgcg actttcgata aagataaagc cccggtcccg gttggcctca taggtgagac
    39181 tgatctcggt atccggtccc cgccaggtgt attgctggtt caggccacgc atcatttgcc
    39241 cgggcagccg ctcgatcttg ccataggacc gttcgagaaa ttgcaacacc tgcgcatggg
    39301 ctttcgcgcc actgtagcga atggtcaccc gcgcaaactg gtcatcgacg gttgagaggt
    39361 gaacgctctc aaccggaata tccgcataga ccggcggacg atcgcgaaat tggtaatcga
    39421 tcgtgtgcga gttggatcgg gtcaccgtca gttccggaat gtcggtgagg ggcacacccc
    39481 aggtgagatg gtcgaaacca tgcgggtcat tcgccattgt gatggccaat gcgggcacgg
    39541 tcaggatgaa gatgagcaca acgagaagtc ccaggcggct cgccatgcgg aggggagagc
    39601 atttcgaaaa cgcgtagggc atggccttca atcgtttctc accaaacgct atcacggttc
    39661 tcgcggtgac ggcaactcgt cggcgctgtt cttgtgaatg gaggacccct tggctataat
    39721 gcgcctcttg tgcccgggcc ggtgagacaa gaggagcagg gttcatgatc gagagcgatg
    39781 tgtttgtgca agcgctcgag aatctgggcg tgaatttttt cacgggtgtg ccagattcgt
    39841 tgctgggcgg tattatcgaa gaattgttga cgcgcaagct ctacacccct gcggtgcgtg
    39901 aggatgaagc ggtcgccatg gcggcgggcg cctacatggc cggaaaagtt cccgccgtac
    39961 tcatgcagaa ttccgggctc gggacgtcgt tgaatacgct gatttcgctg aacatgatgt
    40021 atcgccagcc ctgcatcctt ctggtgtcct ggcgggggtt cgagggcaaa gacgccccgg
    40081 aacatctggt gatgggtgag accatgccgc agttactcga tacgatgaga attccgcacc
    40141 ggacgctgtc ggaaccgacg atgatggacg acctccggtg ggtggggcag accgtgatga
    40201 agcagcgggt cccggtcgcg ctcctgatca agaagggcat tatcaagggg ttgcatccat
    40261 gaggcccgaa caaggcacca tgcagagccg ggcgcaggcc atggcggcgc tgctcgaatt
    40321 gctggccgat cagccggtca tcatttgcaa cggatttccc tcgcgggaag cccacaagct
    40381 tgccgatcgc cccacccatt tttacatgat cgggtcgatg gggaacgcgc cggccattgc
    40441 gctcggcgtg gcgctgtcca aaccgggcaa gcaggtgatc gtcttcgacg gcgacggcaa
    40501 tgtcctcatg gggatgggca cgttggccac agtgggcgcc ttgaagccga agaatttcat
    40561 tcatgtggtc ttcgacaatg aagtctatgg ctcgacgggg aatcagccga ccatctcgaa
    40621 tgtcgtgcag ttggagaaag tggccagagc tgcggggtat gtcaatgtcg agcgtgtgct
    40681 ggaccgggac gacctcgtct acgaatttaa ggacatgttg aaaaaagacg gccccagcat
    40741 gttgctggtc aaggtcaacg agtttgtcga ggatgccgga cgggtgccgt atgagccgcc
    40801 ggccatcacg gctcgattca tgagtgcgat tgaatagcac cgatgtgctg agtgcgatgt
    40861 gtggggccgc tccgctcagt tccgatccac agcctgaggt acgaacatga tactgctgaa
    40921 tccaggtccg gtgaacgtga gcgaacgggt gcggcaggcg ttgttgcgcc cggatatctg
    40981 ccatcgcgaa tccgagtttt cagatctgtt gggtcgtatc cagcaaaaac tgttggcggc
    41041 ctttgtgccc ggcgccgaat ccgactacgt ggccgtgttg ttgacggggt caggcacggc
    41101 ggccgtggaa tccgccgtca tgtcctgcct gccgatgggc aagcggatgt tggtcctcaa
    41161 caacggcgtc tacggcgagc ggctctccag catgatcgga ctgcatcgac tcggagtgtc
    41221 cgaactcaag tcggagtggc acatcaagcc ggacccggag cgagtccggc tggcgctgcg
    41281 tcagcatccc gaggtgcatg cggtctctat ggtgcaccat gaaacgacca ccggattgat
    41341 caatccggtg aaagaaatcg cggaagtcgt cgatagtttg aatcgggtgt ttgtgctcga
    41401 ctcagtcagc ggcctggccg gagaaccgat cgatatcgcc gggtctcaca tctatatggt
    41461 ggcggggact gccggcaaat gtattcaggg ctttcccggc gtgtcgttcg tcctgctccg
    41521 caagggattt ctggaacgga tgcgcaacta cccgaagcgc tcctggtacc tccatttgac
    41581 ccattatgtc gacgaccagg gcaaagggat tgtgcccttt actccggccg tgcaaattta
    41641 ctatgccttc gaagaggcct tgaacgagtt gctggaagag ggcgtcgcca atcggtgtca
    41701 acgctataag cgaatggcgg tgcgcatccg ggagcgaatg gcgaacttgg gggtcaaggc
    41761 attgttgccg gcggattcgc tctcgaatac gatcacggcc tttcacctgc ctcccggcat
    41821 cagctatgcc accttgcacg atcaattgaa ggcccgcggc tacgtcatct acgcaggaca
    41881 aggacaactg gaatccaaga tcttccggat cgccaacatg ggcgccttga ccgagttgca
    41941 gatcgacggg ttcctggcgg cctttgaaga agtggtgaca ggagcggttg cccgcgcatg
    42001 aaagcgatca tccttgcggc cggagtggga aagcgtctct ggccggtgac ccagcataaa
    42061 ccgaaatgcc tgatcgagat cggcgggcag accttgctat cccggtatct cgaatccctg
    42121 gcatcggtca agatccggca ggctaccatc gtggtcggtt ataagcagga aatgatcagg
    42181 gccgcagtcg gctcgcattg ccacggggtc gatctctcct atctggtgaa cgaggagttc
    42241 caccggggca gtatttcgtc gttgtggatc gccaggacgg cattgtccga cgacgtggtg
    42301 atcatggatg cggatgtctt gtttcatcgc gaaattctgc gtcggttggt ggcctccccc
    42361 tatgagaact gcctgctgat ggacgacacg gtgaagcaaa ccggcgagga atgtatggtc
    42421 gtcgtgcagg gcgatcgggt ggttgccctg agcaaaaaga tgccggagcg gtacgatatt
    42481 gcaggtgagg gggtcgggtt tctcagggtg cgacgggccg atacggccca tgtggtcggc
    42541 tcgctcaagg ggtatatcga ccaggaccgg tgggacatgg aatacgaaga cgggttgttg
    42601 cagtatttcc aggacgtgaa ggtcgggcat gagaaaatcg gcgggttgcc gtggacggaa
    42661 atcgattttc ccgaggatgt gacgaaggcg gagcgggagg ttctgccgcg gctctaaatg
    42721 gtggctcccc tcgtgtgaat cgtgaagcgt atctcgtaag atatccggcg cctatacgct
    42781 atcactatgg atctccagtt tctttgcgag atacgaacga cgaacgacga gtccgacgat
    42841 gagtgaaagt acactgcagc gcggggcgga actccaggga ttgagcacgg ccattctgtt
    42901 gccgggcgcg ggtctgtttg gggatcggcc gggacgtggc ctgaccgacg tcgggccctt
    42961 gacctctatc ggagggttga gcctgtttca acgcaccgtg ctcacgctgc agcggggcgg
    43021 catgcgccaa ttgatcgtgc tggcgggatc cgatgaagag ttgctcaagc acgccttggc
    43081 acggggtgcc cgtgtgacga ttcctgtgcg ttggatgccg gtgcgggagt tcccgctcga
    43141 tgatccgcgg acctgggaat cgctggccac cgaagtgcga ggtttttgtc tgatcgccgg
    43201 cgtgcaggcc gtgttctcca agggcttgat cgaacacctg cggcagtccg ttcgagacgg
    43261 cgaggcactc gttgtgacgc gggaagccgg tccggttgag ccggcgctgg gccgccgcaa
    43321 tcccgcggtc gcgcttcagg aagggcggct gatctcgttt cacaatcatc cgggacagga
    43381 aggccaccag gttgccgccg atttggtggt gttgccggcc agtattttga ctccgccgaa
    43441 cggagcggcc gcatccccat ccggtgccgc agagccggcg ggcatgattc cggtacgacg
    43501 ctggctggaa cgggcggcgg tggaagggcg tgtgcgggtg gtggcggctg cggcccacgc
    43561 aggcctgtgg taccgggacg tgtgggatca taccagcgcc ggattggcgg agcgcaccct
    43621 cttccgttcg ttgaagggcg aggctgaggg attcgtcgat cgctatttca accggacctt
    43681 ctcgcggctg ttgactcgac tgtttctgcg gatgaaatgt tcgcccaacg cgatcaccat
    43741 ggtggcgacg gcggtcggga ttctgtctgc ggtggggttc ggcatcggga cgtactcggc
    43801 cgggatcgtg gccgcattgc tgtttcaatt ggcggcggtc atcgattgtt gcgatggtga
    43861 ggtggctcgg ctcacgttta ccgaatcgcc gttcggtgcc tggctggata tcgccatgga
    43921 caacgtggtg catatcgcca tctttgccgg aatcgcctgc ggcgcgtatc tccggcaggc
    43981 cggtaccgaa ggcgcctggg ttccgttggc gttgggcggc gcagcggtgt tcggcaatat
    44041 catgtctttc tggctggtca caaaggcgca aaaaatcggc gcgacgcgtg gctggaacac
    44101 acccaagcag gcggcttggt cggacttcat tctgaagaac gtcgcaagtc gcgatttttc
    44161 cgtggtggtc tttctctttg cgctgctgga taaactggat tggtttctgt ggatggccgc
    44221 actcggctca accgtcttct ggttgatgat ggtctgggtg attcgtccct cggcacgggc
    44281 ccgtgcttaa ggcggccctc cttcttctcg gggccctgac gttctctgta ctggtctggc
    44341 atatcggcat cgggcggatc tacgaggcgg tcacgcaact cggtcccgcc gccatgctgg
    44401 tgattctcct cccctctctg ctcatgtaca ttctggaagc ctacggatgg ctggtcacgc
    44461 tcggcgcgtg ggcgaaagac gtgcctttct ggcgagtgct ggcgattcga accgccggag
    44521 aagtggtgaa catgaccact cccacggcct acgtgggtgg agaaccgctc aaagcgtttt
    44581 tgctcaaacg acagggtgta ccgatcgtgg aggggttggc gtcggtggtg acggccaaga
    44641 ccacgatgac gatcgcccag attctcttca tcctggccgg gatcgggttg gggttttggt
    44701 tgttaggcgc cggcggatcg gccggccaga ccatcacggc agggttggtg agtgtggggt
    44761 tgctcgtgtt cggcatcggt gcctttgtca tcgtgcaacg gcaaggcatg tttgcctgga
    44821 tgttgaacct tcttcgacgc atcggcctgc gactgcagta tttggagtcg cgagagcaga
    44881 aactccttga actcgatcgc acgattgccc acttctacgc gcaccggcga acggccttct
    44941 tggtatcgac cggcctcttt cttctcggat ggctggccga ggcgttggaa gtgttcgtca
    45001 tgattgtgtg cttgggacaa cccattacag tgttgtcggc cattgcgatc ggcgcgctgt
    45061 cggtgtttat caagggcggc acgtttttca tccccggcag cctgggcgcg caagacgccg
    45121 gcaatctgtt cctgttgacc gccttcgggt atggcgatgt cacgggtatc accttcgcgc
    45181 tgctgcgaag attccgtgaa ctggtatgga tcgggatcgg gttggcgtgt ttagtagtgg
    45241 tcgggaaagg acagaatgat acgcttgaag accccgccgc ccagcgatag cgctcccttc
    45301 actctagcag gatgcggaaa aagtccgcca gcggcgttct cgcatcgctc agacgctcac
    45361 cgtacggcag agagtacgat tcgcctcttc gctcgctgcg gccttgctgg acggcctttt
    45421 tgcgcatcct gcgaccgatt ctgataccaa cacgccacat gaactgatcg cggcgtatga
    45481 cgcaacaatc gagtttttcc gcagcctgct agagatgagt cgccaggtga gtccaatctg
    45541 cctttctgac gaagtgcaat ggctctctct ttcgtaatga ctcttgtacc tcctacacct
    45601 ggagggctta ttgaatggcc tcgtcccact cttgtccaaa ataagttctt aaaatgccgg
    45661 gggtctgtga gtaggatagg ctgttccgcc acatccattc acggcgtatc cgcgccgtgt
    45721 tactcacacg ccggaggtgt catatggtcg ccgtcgcgag ttgtttgcgc aaatcttagc
    45781 tttgattgat cgtgcggcct ttgctcgtgc gatccagtac ctgctgggct ctagagcgtt
    45841 tttggtttat gtctttacat agcctgaaga cgcgaagtag cgcgtgcatt cagcgggtgt
    45901 gaagcggtcg agcagcatgc cgatctcagc ccagagcgca tccacggttc gaggggcggc
    45961 tttgcggagc agcgcgttga gtttggcaaa cagtttttca atgggattga gatcggggga
    46021 atacggcggg agatagcgaa tgtgtgcccc caccgcctca agcgcctctt tgacgcccgc
    46081 cactttgtga ctgcgtaagt tgtcggcgat gacgatatcg ccggggtgga gcgtcgggca
    46141 taagaactcc tggacatatt tgaggaacag ggtgccggtc atggccccat ccgccaccat
    46201 cggagcggag atgtcatcat gacgcagccc ggcaacgaag gtggtggtat gcccatgtcc
    46261 gtgcgggaca gaggcgacgc accgttcacc acgcggggcg cgtccgtagc gccgtgccat
    46321 gttcgtcgaa gcccctgttt catccagaaa gaccagcttc gtgatgtcca gagcgggttg
    46381 ggctgcctgc cactcacgcc gtgcggcgtg cacgtcggcg cgatcttgct cgctggcgtg
    46441 taggggtttt tttacgtgtg agcttccatt tgtcgagttg atgccacaag gcggggacct
    46501 tcatcgcaat cccgtgtgcc gctaatcggg cacacatctc cgcaagggtc agatcacctg
    46561 tttccttcaa ccacgcccgc agtgtcggct ccatcccgac caatcgggac tgccgatggc
    46621 ctccaattgg gagactgctc cgctgccccg tctgcgtgag ccggttccgc acctgataca
    46681 cccagacccg actgacttca aatcgggcgg caatggctgt cggacgctcc ccacgttcca
    46741 atgctctcaa cacacggtcc cgtagatcct gcgaataggc atccatcctg ttgcctccat
    46801 caagaaacca atggagggca ttctacagag tgtatgcact taaatcaaaa attctctagt
    46861 gaccgacggg gatactaacg acgtgcgcat cgcccaccaa ctcacatttc caccaggcac
    46921 catagtggcg atggatcggg ggtatctcga ctatgtccta tatcatcgct ggacgctcgc
    46981 cgaggtgggg tttgtgacgc gtccacggac gaacatgctg cacgaagtcc tggagcggcg
    47041 gccagttccc atgcgaggcc cgatggtggc cgatgaggtg attcgcctca ccggcctgca
    47101 ggccgctgtc ccgtcccttt gcggcaggtg accatctggg acgagacgca gcagagacgg
    47161 ctcaccttcc tgacgaacct catgcatctg gccgccagta cgattgccgc catctataag
    47221 gagcgctggc agatcgaact cctcttcaaa gctttgaagc aacacctcaa gatcaagacg
    47281 tttgtcggca ccagtgagaa cgccgtgcag gtccagatct ggattgcgtt gattgccatg
    47341 gtgctgttga aatatctccg gctcaagtcc acctggccct ggagtctctc gaacctcgcg
    47401 gccttgctcc gcttcaatct gttgacgtat cgagacctgt ggacgtggct caatgcgccc
    47461 tttaagcatc cgcgatcact ccgatgccct tgcaaacagg gctttttgcc gcataatctt
    47521 ggacagcacc cagaccagac aacctccttc gagaacaggc gccgaagtcg tctcaaacgc
    47581 agcattcatg caggagacac acagaggggc ggattatttt ggacagcagt ggcctcgtcc
    47641 cctctatgct tgcctcaagt ctctcgacag atgccgcagg catgcaggag gggagagtca
    47701 gcagggtgtg ccccttgatg actcgctcga acatcagtcg cgggtctaag ccagtaggag
    47761 gataattccg gtgaatcgtt tgccatggcc gtcgtagtga tcgttccagt tcacaacggg
    47821 ggcgaacgat ttcacgcgaa tatggattca atcaggcgtg ccgagcctcc gccagatgaa
    47881 gtcatcgtga tcggcgatgg cgacactgac ggatcctccc agtttgcaga agcagccggt
    47941 ataaccgtgt atcgctttcc tacgccgaaa gggcctagcc gcgccagaaa ccttggcgcg
    48001 tcgaaggcga tgtcggaact gctcttgtca gtcgatgtcg ctgggacagt tcccgaaaat
    48061 gtgatcgatc gggtgagaga cctctttgcc cgagattctg acgttgcggc aatgatcggg
    48121 tcgtatgatg atcaaccggg ggagtcgaaa ttttctttct cagtaccgga agttggtgca
    48181 ttattctgtt catcagaacg gtcgcgagga ggcgtccacg ttttggggta cctatggtgt
    48241 ggcgtgcgga gttcttggtg tactgttgct gtcccccctg gcagccggtg tttctggttg
    48301 agaccgttgt catggggctg atcctgttta tcctcaacta tggagcacga taatgccagc
    48361 agttaatccg cgagaccaga aggtcgtgat catcggaggg ggaccggcgg gactcacggc
    48421 ggcctaccag ctgtcgaagg tcggtgcgaa gtccgtggtc ctcgagaaag atgcgatcct
    48481 cggcggcatt tccagaacgg tggagtataa aggctaccat tttgacatcg gcggccaccg
    48541 ttttttcacg aaggtttccg tcgtggacaa gatgtggaga gaggtgcttc ccgagggcga
    48601 cttcctgcat tgccagcggt tgtccaggat ctactataat agaaattttt tcttctaccc
    48661 ccttaagcca gtcaatgcct tgttcgggct aggagttttc aacagtctgc tcatcctgat
    48721 gagttatgtc tgggcgcaac tctttcccca gaaaccggag gataattttg agtcctgggt
    48781 ctccaatcga ttcggcaagc ggctctacaa aacctttttc aagacctaca cggaaaaagt
    48841 ctggggcatg ccggctaaca cgattgcagc tgattgggcg gcgcagcgga ttaaagggtt
    48901 gtcactgctg gtggccgtca aaaatgcgct gatcaaacaa caatcgacgg agaagggggc
    48961 ggtcgtcaag accctgatcg acgcgttcga ttatcccaaa cgcggccccg ggatgatgtg
    49021 ggaaatcgtg ggagagttag tgaaaaagga tggcagtcag gtgcatctcg gcgcctcggt
    49081 tgacaggatt cgttgggcag gcaatcgaat tgatgggatg gatgtctcga tgcagggtcg
    49141 aaatgaatct ctgacaggca cgcactatat cagcagcatg ccgatcagag aactgattca
    49201 taaaatgaat ccgcctgcac ccaaggaagt catcgccgca gctgaccgac tgaggtaccg
    49261 ggactttctg accgtcgcac tgatcgtgaa taagaaggac ttgtttcccg acaattggat
    49321 ctatattcac gactcgcagg tcaaggtcgg acggattcag aacttcaaga actggagtcc
    49381 ctatatggtt ccggaccaga acaagacctg tttggggttg gagtattttt gcttcgaggg
    49441 ggatgggtta tggacgatga gcgacaagga cctcatcgaa cttggaatgc gcgaactcga
    49501 attgatcggc attgcgaagg cggaggatgt cgaagacggg tcgattgtgc gaatgccgaa
    49561 agcctatcct gtgtacgatg cggactatca agaggctctc acctgtatcc gggaatttct
    49621 ggacggcttc caaaacctgc aagtcgtagg gcgaaacggt atgcatcggt acaacaatca
    49681 ggaccactcg atgctgacgg ccatgctggc agtagacaat attcaaggcg caaggcatga
    49741 tctatggcaa gtgaacgcgg agcaagagta ccacgaagaa acgaccagcc gcgaagccga
    49801 cgccaaggtg caacttggcc agcttgcggc cacgcagccg caagtcccgc aaccgatgac
    49861 gccggccgcc agtcagagtc tcgttctggc attcgccaaa ctcgataagt tggcctttgc
    49921 gaccgccctg ggaaccgtct ccggtctgat tgtggcgatc gctaccttgt ggatagcagt
    49981 caaaggcgga gagcgagccg ggcagaatct gcaaatgttg ggacagttct tccaaggata
    50041 cacggtgacg gtgtcgggtg cgctcgtcgg gtttgcctac agctttacgt ccgtcttcct
    50101 ctggggctgg ctgtttgcct atctcagaaa cttcgcgttg ggcttcgtga tctaccgcgc
    50161 cagacgagag gccgagctga tgtcattcag acaattcctg gatcgctatt gatctatttg
    50221 atgccagagg agtatgacca tgccgaccat gatgcagctc gaagacgaga aactctttag
    50281 cgcgatcctc cgtatcaata gtaaaatgtt cgggctcatc ttgggtctct tgatgggatt
    50341 ggcgatcttc attgccacca attggctcgt cttcaaaggg ggatataccg acgaaaccgg
    50401 tcattacatc gtcgggccgc atttgcagtt actgagtcag ttttttatcg gctattccgt
    50461 ctctttcgtt ggcagtttcg ttggatttgc ctatgggttt gcttgcggga ccctggcggg
    50521 ggcttcgatc ggcatcattt acaataaact tgttcaactt agaaactcag cctcatgagt
    50581 gttcctatga aattcggact catcggcgcc ggatgtatcg gtcagcttcg ggccaaagcc
    50641 ttggctcagg ttgaaggctg tgtgttgtcg gcgattgtcg acgtcgatcc ggtgcgggca
    50701 cgagccatgg cgtcatcttc agacgtcaag atattcaaag atgttcagga gatgctcccg
    50761 caggcaaatc tggatgccgt gattgtgtcg acaccacctc agtttcacga ggatcttgtc
    50821 gtcagtgcgt tcaaagccgg gaaacatgtc ctctgcgaaa aaccgctggc gaattcggtg
    50881 gaggcttgca ggcgcatggt cgacgcggca cgggcaagcg ggaaggtgct tgcaactggg
    50941 tttaatcatc gctacttccc gcaaatccaa tatctcaagc aagccatcga cacagggctc
    51001 atcggagagt tggaccatgt ccgagccttt gccgggcaca cgggtctctc tgaatttcgg
    51061 aactcgtggg aatacgatat aaatggtatc ggtggtggcg ctttgatgga tgtcgggatt
    51121 catatcatcg acctcacgcg gtatgtgctg ggtgaggtgg atgaggtcta cggcttcacg
    51181 tccaaccgtg tctggaatct gggcgcatcg gaggacaatg gtattgccct gttgcgaaat
    51241 ccccagggaa aatatgccgt gttgcaggcc acgtggacgg agtggaaggg ctatcggttc
    51301 tatctcgaag cctacggcac taagggcatg gtgcgcgcct actatgcacc catgatgaat
    51361 cttctgatca cacaggacaa gccgggtgga cgccggaagc ggacggttcg cttctatccc
    51421 atgacgatgc tccgggagaa attcatcggg tggcaatcgt cggtcattca gatgttccgg
    51481 caggagttca cagatttcgt ccgcctgact cgggggaaag agagcgcgat tgcggacggc
    51541 gttgccggtc ttcgagcggt ggaaattgcc aacgccgtgt atcgcagcag taaagaacac
    51601 atcgcgattc ggttggccgg gaatatctaa atcttgctgt agccgtggat gcaccatgac
    51661 aaccgttaac cctaaccatg cagagttcaa gacccaagac gcggcaagtt acaacccagt
    51721 cgcgcagagc ttcgaccggt tcgtgactca attgtctgct ccggtcgctg cccgtctcgt
    51781 cgcgcttgcc gggctcgcgc cagacaatca cgttctggat gtcggtgcag ggacgggaat
    51841 agtcgcgctt cgtgcggttg cagagatggg gccatcgggc agggttgtcg gcatcgactt
    51901 gtccgacggc atgctggagg ttgccaggca gaacgcgcga gggcgaggcc tcgcagatcg
    51961 tgtcgaattt cgcaagatgg atgcggagtc gctggactgc aaggatggat cgttcgacgc
    52021 ggtagtctcc ttgttcgcct tgcgtcattt tcctcatcct gagacggcct tacaggaaat
    52081 gcatcgcgtg cttcgtcctg gaggccggtt ggtgttggct gtcgggagcg gcgcaccctg
    52141 gctctctctc gtgggattgg tccatcggct gaagctccta ccgcacgttg tgcgccgttt
    52201 tcagggtaag caactcgtgg catgcaagtt tttggacggg atggtcgcgg cctatgtccc
    52261 gtcctcaggc aagagcgaag aggcaggttg gacgcatgaa catacgcata tgcagcaatc
    52321 ggtaccggca tttattcgag cagccggatt tcagaatatt cgctcggagt ggcaggggca
    52381 acagtccatc atcaatacgc cagaagagtt ttgggagctg caaacaacgt tttcttccct
    52441 gtccagaaaa cgtctcgcca cagtgtcatc tgaggtcgtt gcgcgtgtgc ggcaggactt
    52501 tatgaacacc tgtcgcgagg tcctgtctcg cggcggggaa ttggtgtatc caactggagc
    52561 gttgctggtg tccggtcgcc gcgcatgaca cgcccttagg gcgtgatgac aattgaattg
    52621 tagcgatcat gccagccaga tacaggcaca agccagactg agcatggaca tgaagttctg
    52681 cgaaagcttc tcgtatcgtg tggcggtgcg cctgaacggc ttgagtcggc agaagaagcg
    52741 ttcgacgagg ttgcggtctt tataaaggtg acgatcatag acacgctgag tcagacgatg
    52801 actgcgtggt ggattcacca cctgtgcgcc ctgggtttca atgagatcga cgaatgcatc
    52861 ggcatcgtag cccttgtcgg cgatgacgca ttgcgcctca atccccttga taagggccgc
    52921 cgcttcggtt atgtcggccg cttgcccgcc ggtcaggcgt atgcgcacgg gattgcccaa
    52981 ggcttcgaca gtcgcatgaa ttttggtgct caggcccccc cggctgcgtc cgatggcttg
    53041 atcgctcatt ttggggggac gccggtagcg tgctggtgaa ctcgcacgat ggtcgagtcg
    53101 acaaacaact gctctagatc ggcgtcacgc ttgagtgcct cggccatccg cgcccaaacc
    53161 cctttcttgc tccagcggga aaagcgcata tacgctgtgt gccacgctcc gaagtgctgg
    53221 gggcaggtcg cgccacggcg agcccgtgcg ggcgatccac aatgcttcca ttgatcgtcg
    53281 cgcaacatct ttcggtctat tctcgcaaac tcccaaaagc gagaatgtat agaactttgt
    53341 catttgtgag cagcccctag taaataaacg gaatctcggg ctgcagagaa gcgctatggc
    53401 attcatgagc aagagggtca tccggcagct ctgtattgcg gcaatcagcg gagtgctgac
    53461 ggtaggattg ttcgttgccg tggattcgaa ggacgcgacc ttccgctgga gcatggccac
    53521 ggcatatgtc gggttggcac tcatcgggct ctcgcttatc attggtccca tcaatgtttt
    53581 gcgtgggtgc tctaacccgc tcaacaccag tttgcgtcgc gacgtcggca tttggggtgg
    53641 cattataggc ctcgtgcata cggtcgtggg cttgcaggtt catatggcag gacggttctg
    53701 gctctatttt ctctacccgc gtgaagagag ccatctcgtt cccttgcgat acgacctctt
    53761 cggtttggca aattattccg gtctcggaat ttccctcgtg ctggcgctct tattgggttt
    53821 gtcgaacaac gcagcattga ccaagctcgg ctctcaccga tggaaaacgt tgcaacgatg
    53881 gaattacgcg ggatttgcac tcttgatcgt gcacggggcg gtctatcagc tgcttgaaaa
    53941 gagaatggcg ggatttgtac tcgtatttgc tgcggcaatt cttcttgtgg gagcgctgca
    54001 aactgcaggc tatcgcaaag ttcttcagca gaagaatcct ggcgggcaac cttccgtcat
    54061 gagttctgac aggtagtcat ttcatagcgt tcagtttttt gctatcaaat tgaatccgtt
    54121 gctccgcatc gatcgaggaa ttggacggcg tgatgctatg cgcgcgtgtc cgttgtgatg
    54181 agtgttatca gggcatgact agtggcctgt aacagcagat tatgtactga gcctgcggtt
    54241 aagatggatg tttttaggct gcccccatcg cgccagagcc ggataaggta gtctacaatc
    54301 gtctgttaca ggccacggag gaggaaagaa tgagaacact tgtcgtcaac gccttcagaa
    54361 tggctcagca gcgaaccggg ataggccgct atatcgagtt cgtggcacag caatggagcc
    54421 ggatgaagat cccgttcgat cgagtggtgt tgatgagccc gaagaacata gagatgagtg
    54481 gattcgatcg cgtcacttcc gttgaggtca aggtgttcgg gggacaatat ccagccgcca
    54541 tttgggaaca ggtatgtttc cacagagcgg caaaaggtgc tgcgatattg ttttgcccct
    54601 cctacatctg tcccttgttg catcgtgggc gcatggtgtt ggcaaatcat ggcttctatg
    54661 aagccctacc aggcgagttt tcctggttca cccgcctccg tgcgacgacg ctgcaacggt
    54721 acagtgcgaa gtgtgccgaa cgcgtgatcg cgaactctct tgccacaaaa gccgatctcg
    54781 tgcgattttt tggcattcca ccggagaaga tcgacacgat ttatccggcc gcacacgatt
    54841 actacttcca acgccacgat ccggcggcga tcaaagaaga agttattcgt gcgctgggca
    54901 aagaggttcc ttatgttatt tttgtcggga agcttgcgaa gaggcggcat gttccgaatc
    54961 tcatcgaagc cttcgccatc gtccgccgac aacataactt gccccacgct ctgttaatcg
    55021 tcggaccgaa caccacgaat gtcccgattg ctgagatcgc gcaacagcat ggggtcgggg
    55081 atgccgtggt gtatcgccct tatgcggagc ttgagccgtt ggcacggctg tacgccggcg
    55141 cggatgtgta cgccctgccc accatctacg aagggatctc ctataccatg ttcgaggcta
    55201 tggccagcgg tgccgctgtg ttgacggtga agcacccgac attggaagag ggcgcaggag
    55261 atgcggcgct ggccatgccg actccgtcag tagaagattt ggtcgaggga ctttcgacgt
    55321 tcttgctgga tggggcagtc cgtcgcaagt atgccgaaca gggccgtgag cgggctaagc
    55381 ggttttcatg ggagatggtg gcacgggaaa ccatgcgtat cttggatcgt gtggcggaca
    55441 aagaggatcg gaatggatat tgctaaacag aagaggatgg ctagatggcc gacatgagcc
    55501 agatagatcg gatcgttgat gcttaaaatt cttctgggaa tcggggcccc ccaggctctt
    55561 gctgtactgg tgaactttgc tcgaacgaag gttgccgcgg tgttggtcgg ccctgaaggc
    55621 atcggagtca tcagcatcat cgatcaaact atccagtttg tggcgaacct ctgcacccta
    55681 aatttttccc gttcgacggt gaagttcctt tccaaagccc atagcgaggg aggcgatgcc
    55741 ttttgtgatg ggtatgccgt ggttctgaaa gcgttacttg tgctcggcac agtcggtgcg
    55801 ctggtgacaa caagtctagt gctgttcaga atcgatctcc tccccgccgt aatagcaccg
    55861 tataaaatct tgcttctggt ggggttacta ggtattccgg ggatggcgtt ggcctcattt
    55921 atccccaatg cactagcggc ggcccagtat tcctccggat cggcgcggtt gaccgctttg
    55981 acgggcctcg tcatggcatt ggcaagcaca ttcgggctcc ttgcaggagg gattttaggt
    56041 ttctatgtcg cgacggtgct cattgtaacg gtgtgggcca ttgtggtcgt cgaatatctt
    56101 cgtcgcgtga tgggatggtc gattactcag tgcgctgttg gcattgcggc tggttttcgc
    56161 aggctaggca atgtcgtctc tttcagcatt ttgttctatg ccgcagcttt cacctatcaa
    56221 ggagcattct taatcacacg gtattccgta cttgagaatc ttggcgaggc cggggccggg
    56281 cttctgcatg ccggtatgtc tgttgccctt ctcatgaaca tggttctgat gccgctcact
    56341 gaattatttt ttacaccaag agtcacccgg gtcggaccaa aggaagaaaa gtttcaagtt
    56401 gccatggaat tcctgaagca actgaccatg atcgtgacga tattttcgct ccctgccatc
    56461 ctcttccctc acctcgtgct tgatctggca ttctccacga agtttttagg cgccgcgcaa
    56521 tatatgttcg tgtttatgct gtggcaaagt ttcgccctgc tttccggcgt ctgccaagct
    56581 ctcttgattg gactggacga catgatctcg tatacggcca tcacatgctc ggggaacgtc
    56641 ctggccgcct gttccgcggt ggttcttgtg ccgcattatg ggggcatcgg ggcggcggtc
    56701 ggcttcgtga tagcgagttc gctggtgttg ctcggcacct ttgttaggct caagctctgg
    56761 cacgggtttg ccttgccgag gaatcttgtc ttcctgatgg ggtatagcct gttggtcacg
    56821 ctggtgctcg gcagcatctt tcggcattac ggcgcgtggc acggttcaac cctactggcg
    56881 aaagctgcag cggcgctggc caccgtcatc ggcctgttgt tatttttgag caggaatgac
    56941 aagctcgcat tggtgagctt ctttcaaaag gtgcgatcgg gcccgtggta gctcgcgggg
    57001 agcgaatgca gcattctcaa tttccagtgg agggttctga tggtttggct tgcggtcatt
    57061 cggttgggga gcatcgtgga cgctgcacgc ctgaccctgc tgccggagtt ccgcaggaca
    57121 tatccccata gttgatgaat atgccattcg tgagtcagcg acgagtaggt tagttgtcac
    57181 aaaatctggc ggcgtaatcg acataatggg gtcttgaact atgacccagg catccacggg
    57241 gtcgaatggt caccaacaaa taacgcctac gatcatctgt gtccatcgga tggtcgatgc
    57301 tgagctgcac gccaggagaa tttcgcttga acagtaataa ccgcttccaa gaagtcgcga
    57361 aaaagatttc tgtcgtcctg ctgaatggtg tcttgctcgt ggcgatgccg gcctgtgcgt
    57421 cggtcaacct cgggcgcgat agcatgccaa caatggtcga agtgacgatc ttccctcagg
    57481 agcctcgtca gacgatggag ggatttggag catcaagtcg agtgtgggac gatccgcatg
    57541 taagtaatgc gggctccact tcggtgcccg ctgcggcaca ggagaatgtc ctgactctcc
    57601 tgtacacaga tttaggcctc acgcgcatgc gaccggtgtt ggacggaggg gtggaaccga
    57661 tcaacgacaa tgtggatccg gcggcctttg actggacaaa attccatttt gaatggaaac
    57721 ggaccgatgc acatattagc ctcgtggagc aagctcgagc aaaagggatg acgacattct
    57781 ttgtttcacc agttacgctt gaggcgtgga tgacggagga caatcccgat gagtacgtcg
    57841 aatgggcctt ggcgatcatg ctgaggtggc gcgatagagg agtggaatta ccgtatttct
    57901 cgatcatcaa tgaacctggg tataagcgca gcgggatttg gtcggctgaa tggatgagaa
    57961 cggttgtgaa gcggttgggg gccagaatgc ggcaggaagg gtttcgaacc atgttggtca
    58021 ttccggacga tctgaatccc gccgaagcct acaaacgagc gtccgtcgtg cttcgtgact
    58081 ccaaagcccg ccgatacgtg ggcgcgctgg cctaccactt gtacgatacc aaggagaccg
    58141 aactcgcgca cgaccttgcg cacatgcgga atctcgcggc cgaatacgga atccccatct
    58201 ggatgacgga gtttagcgtt cccaacgaca tgactttttc cggtgccatg gagtggatga
    58261 aaatcatcca taagctcgtg gcaacgtacg atgtcagcgc tgtggattat atgtggggat
    58321 tcttcggagc ttgggaaagg aaccacacaa tcctgagcat cgagttttta gacgggaaat
    58381 acgtgcaaca ccatgtcgga ccgctctact atttgacagg gcagttctcg aaattcgtgc
    58441 gtccgggaca ggttcgagtc gaaacgcatt catcgagcca agatctgttg gtgacggcat
    58501 acaagtcagg ggcacagtcg gtgatcgtgg cgatcaatgc cggtcaggag gaccgccgcg
    58561 taaatgtgac ggtcacagac gccgtggcgc cgcttcattt ctcggcgatc cggaccaccg
    58621 tccgggaatt ctggagcgaa ttagaaccga tcgcatcgga tacatccacc ttcaatgtcg
    58681 cactgcctgc acagagcgtc accacatatc gcggtggatc aattgattcg gccggtaccc
    58741 tctccggtgt cggcataaag aagagcgcac aatgattcgt cttgccgtca tcgggttggg
    58801 agcggtcacg agaaatattc atctcccggc ctatcgccgg ctcaagggtc gcgtgactct
    58861 cgtcgccggt tgtgatgtgg atcttgcgac gagggcggcg atggaaaaga ccggtctttt
    58921 caaaaggatc tacgacagcc acgaggagat gcttgacaag gcgaagcccg atgttgtgag
    58981 tatttgtact ccgccgtttt tgcatcacca gcaatgcctc gatgcgcttg agcgtgactg
    59041 tcatgtcttc tgcgagaagc cactggcgga aagtttggcg catgccgacg agattattct
    59101 cgcatcgtcg cgatgcatgc ggtcggtcgt ggtcaacagt caattcccga atatgaacat
    59161 atatacggcc gccaagaaac agatcggctc tccggagttt gggcggctgc tcttcttgca
    59221 tgcctggcaa acctttcggc cgacggccga tacggaaggg gggtggcgcg cgaacatgca
    59281 gcgtagattg tgtttcgagt tcggcgtgca cgtcttcgag ctgatccgac actttttcga
    59341 tgcgacgccg tccacgcttt gggcgcacat gccacgacca attcgtgcat ccaacaccga
    59401 atcgctcaat atcatcgcga tggaatttcc ggatggacgg gcggcatcca ttctcttgga
    59461 tcgtgtgagc aaggggcctg aacgctactt ggagatgaga ctcgacgggg agcatgccgc
    59521 cgttcacacg tcaataggag gggagctgcg gtttgaggtc gggcttcaca cgcgtcagcg
    59581 aaaaccgttc atgggactgc atgtcgtgaa aggtggacaa gcggtgttgc aacaggctga
    59641 gcgggcgacg gtgataggaa cagatggcat gaatccattt gcctccgcga ccgccgtcca
    59701 ttttggcaat tttctttcgg cgattgagga tggccgtgtt ccgtccggga cagcggaaga
    59761 tcacaaggcg acccttgctt tgactttggc tgcctatgat gcggcggagt caaggaaggt
    59821 cgtcgccatt gcaccctatc tatccggagc tgccacgcga aaggaccttt cgtgagggac
    59881 gccgtttctt ggcaatgttg cgtgctcagg taattatggc aactggaccg atcgaaagtc
    59941 ttgccataga gaacagacgg cttcgggtcg ggatcgtcgg tgctggtctt atgggacagt
    60001 ggcatgctta tgaggccagg aaggcacagg gaaatctcgt tgccattgct gattgcaatc
    60061 tcgaagccgc cagccggctg gcgaggacgt ttcaaggcgt cgaggcctgt gtcgatatcg
    60121 aggaactatt gagccggacc tcctgcgatc ttgtccacat ctgcaccccg attgccacgc
    60181 atgaacagat cgccgggact gcgcttgaag ccggcaaaca cgtgctgatc gaaaagcctg
    60241 cgacgcctga tgccgaagga acacaacggt taatccagct ggcggctcgc cgtggggttc
    60301 ttatttgccc ggtccatcaa tttgtgtttc agcagggtgt gatgaaggct cggcggctcc
    60361 ttgatcgcat cgggcatgtg ctgcacctgg aagcaatctt ttgcacggca gggggcgtgg
    60421 gacgccagga gagcgaactc gactccgtcg tcgccgagat tctgccccat ccgctttccc
    60481 ttgcacaggt ctttctcccg ggcggaatgg ttccggacac ctggatgcgt ctccgtccgc
    60541 gcgtgggaga atttcgagcg atggcagaag tgagcggcgt gacggtgtct gtgatgatca
    60601 gtcttcaggc tcgtcccacc caatgtacgt tgaagatctt tggcgccgct ggaaccattc
    60661 atctcgacct ctttcatggg tatgcgttca tcgagccggg ggctgtgtcg aaagaaagaa
    60721 aaattctgcg gccttttgat ttggctgcca ggagattggc tgcggcatcg atcaacctgg
    60781 ccaagagggc cctctccagg gagcgagcct atcctggcct cacgcggctg gttgccgaat
    60841 tttatgcagc catacggacc gggcagtgcg ccccctttac cactgaggcg atgctgaata
    60901 tcgctcgggt tcgagacatc ctggtggagg gtatgagccg tgacggttac acagccgtga
    60961 gtcattcata atggccacat cgccgggtgc gagcgcacag gatcttcctg aactttcggt
    61021 cgtgataggg tctcataatg ccaggaccag tgtggctgca tgtcttggtg cgttgcagaa
    61081 gcaacgcaac gggtgccgcg cagaaatcat tgtggtggat aattcgacgg atggaacagc
    61141 cgccattgtc gcggcgcagt ttcccgacgt agcgctcatg tgtacttccc ctgagcagtg
    61201 gattcctgag ttatgggaag ccggcatcag agcgagccgg ggttctatcg tagccctgac
    61261 cacagctcat tgtgtccccg atgacgattg gatgaaggaa gtcctcgcag cgcaccatgg
    61321 cagcgcctat gtcggaatcg gaggagccat tgagaacgat gcgtcggcaa gtatcaccga
    61381 ttgggcggtt tacctgtgcc ggtatgcgag gtacatgaga ccctttgctc cctatgtcat
    61441 tcgcgacatt cctgcggata atgcgtccta taaacgatgg gcgttggata gttgtacgga
    61501 tgtgcgcgcc cgaggatttt gggagcccga catccatgcc aagcttacgg cggagggcta
    61561 tcaattgttc atgacgccac ggattggcgt acgccatcta cgatcgttta cgttcattaa
    61621 gttcatgcga cagcgatttt ggcatggccg gcaattcggt tcatcccgtg ccatgcgact
    61681 gtccaccgca gtccgactgg cccacattgt cgcggcacca ctcgtcccgt tcctcatgct
    61741 gacacgaatc gcttgccaag tatttcgcag gcggcagcac atcacgcact tgcttcttgc
    61801 gacacccgta cttctgctgt tccttgcgag ttggacgacc ggtcaagtta tagggtacct
    61861 ggcgcccaca ggccatcagg catgatcggc caggagtccc tgaaaaaccc tcgactctct
    61921 gtcattgtgg cctgtacaca ttcccggcta gatctcgagc ttgtcctctc gtctctccgc
    61981 ggccaggaag gggcggacgg tctcgaaatc attgtcgtgg atagttctgg cgatgaggca
    62041 gcaaaccaat caatagttca ggacgaggga gtgctgacgc ttcgatttcc acgggggact
    62101 ccacttcccg ccctctgggg cgccgggatc agacagtctc gtggcgcgat tatcgccgtc
    62161 actgatgcct gctccgtccc ggatagacgg tgggtcttgt ccacccaggc tgctcacgag
    62221 tcgtcggatc tgatcgtcgg tggtgcggtt gacccgatgc cccagcgaag tctggtcgat
    62281 tgggctgcct acttttgcga gtatgggcag ttcatgttgc ctctcacagg gggggatgtt
    62341 tcagaacttc ctggcaataa cattgcgttc aagcgagcgg cgttagacaa gggaacacaa
    62401 ttcgtcgagc agaggttctg gaagacctat tggtgtcggg cgctacagca ggaaggggtt
    62461 cagcttcggt cggtgccttc tatggtggtc tatgatagga agtcctatcg gctgctccca
    62521 tttctggtca gacgatatca tcacggccgc tgtttcgccg gtatgaggat tacgcaggtg
    62581 ccgccggtca tacgcgtcct gtatgcgctc gggactccgc tattgcctgt tctgttcatg
    62641 aggaggattg ctgggccgat catgcagaag cgacgctatc ttccgcagtt tatgtgttca
    62701 ttgcccgttt cgatcttggc agtcgtcagt tggtcgtggg gtgagttctg cgggtatctg
    62761 gcagggccag gacggagttg tgagttcatt caataacatc atcaccaccg gagtagcggc
    62821 gaaccatggg taatcgatca atctctgtcg ttatcgcttg cgtcaatagg ctgccgtcga
    62881 ttcatgattg tttgatcgcc ctggaacgag aacggaagaa ctttcaggtg gagatcctgg
    62941 tgctctgctg ttgccaggat ggcacggccg cgcacgttgc caagcacttt ccagatgtca
    63001 cactggtcca tttcccaaga cggttgggca ttccggaatt gcgagcgatc gggatggaac
    63061 gcgcgacggg ggagattatc tcgatcatcg aagaccattg cattgttcag aacggatggt
    63121 ttcgtgagat tctcgcggcg catgacaagg ggtaccaggt ggtcggcggg acggtggtca
    63181 acggcagcgt cgagcgcctg atcgattggg ccgtgtttct ctgcgagtat agcggggtca
    63241 tgccgcccat tcccgacgga gaagtgtcag gtgttaccgg aaacaacgtg tcgtatcgca
    63301 aggcgatcct cgatcgtgtc gacgaatcgg tgaagaagga atgctgggag ttctttctgc
    63361 atcaggagct cagcaagaag ggggttacgt ttctctccgc gtcttcgatc attgtcagcc
    63421 ataagaaaga atttggattc tggtacttta tggcccagcg ctattactat tcccgatcgt
    63481 tcgccggcat gcgtcggaaa cgcacgtcgc ttccgggcag gcttttgtac tgctgcgcct
    63541 cgccgttgct tccggggctc atgctctgga gaatgacacg ccaggtgctg aacaaacagc
    63601 agcatgtcgg cgctttcgtt tggtcgctgc cgttgcaggc ggcgttcatg gtgagctatg
    63661 cggcggggga atttgtaggg tatctcttgg gctctggtga cagcctcctg aaggtcgaat
    63721 gaacaacgca ccggctgtag gcatagagtt gtccgttgtc ctggcggccg ctcattcgcg
    63781 agccggtctg gaggcctgcc tgacatcatt gaaagaccag gttagcggtg gagagatcga
    63841 tgtcattgtg gcatgtaact gttgcgaggg tgccggtcgt tcgatcgaag aggcttttcc
    63901 atttgtccga ttgatcgagg cacctcccgg taccaccgtg ccggaattgc gcacgttggg
    63961 aatccaggca gccacaggga ggatcgtggc cctgcttgag gacaacagca ttgtggcgcc
    64021 gacctggtgt cgcgcaatta gaagggctca tgctgccccc caagagatcg tcggcggagc
    64081 tgtggaacga gacggcacgc atcgggccgt cgattgggcc gtgtatttct atgagtatgg
    64141 gaaatacatg cttccctgcg tagaaggcgt gagcgcaacg ctgtccggga acaacgtctc
    64201 ttacaagcgg acattgttac aggaaatcca ggatgagttt cgtaacgggt tttttgaagc
    64261 cttccttcat gagcgactac gcaggcaggg cagcaccctc tatatggctc ccgatgccgt
    64321 ggtgtatcac accctgcgct atcgagtcgg taaagtcctc gtgcagacct ttcaccatgg
    64381 ccgccactat gcggggagac gggtcgctgc cgcctcgcgc ctcacgcggc ttggctacgc
    64441 agccggctcg acgcttcttc ccgtgatcct cccgctacgc attgcacagt tggtgctgca
    64501 ccggcggcgg catctccggg agctgacggt cgccatgccg tacctggttc tgtttatgac
    64561 gagttgggcc tgtggagagc ttatgggata cctttgtgga gaaggggaga gtgtgcggcg
    64621 atgggcatag cggcatctgg atcgtcaggc gtgggtccga gcacaagaaa accgcgcatc
    64681 ggcgtggatg cctgctgctg gtcgaatcgc cgggggttcg gacgtttcac cagagaattg
    64741 ctgacgacgc tggtcgcgac tgaccgggag cacgagtaca tcttctttgc ggatggggac
    64801 acggcgcgag cgagcgagtt tcctccaggg gtcacagtcg tggtggcatc gacggaggtc
    64861 tccccgaccg tcgccgcctc tgcctcaggg agtcgctcgc tcaaggattt atgggcgctg
    64921 agccgcgtgg tcgcgcagga gccggtcgat ctgttttact ttccggctgt atactcctat
    64981 ttcccaatcc tgaaccggtg caaagtgatt gtgaccatcc atgacatgat cgccgaccac
    65041 catccggagc gagtcttccc caatcggaaa tcgcgcttgt tctggaagct caagcaacag
    65101 gctgcactct ggcaatcgga catgattcta accgtttcaa gccatgccaa gcagcagatc
    65161 gtcgagtatt gccgggttcc tgctacgcga gtggagacga cgacagaagg cccgagcgat
    65221 gtgttcattc gcctgccgaa tctggaaggg gccacagcgg tcttaccgaa atatggattg
    65281 agatccgggg aaccgtttct aatctatgtc gggggcatca gcccgcacaa aaatttaggg
    65341 gtgctggtcg atgcgtttca tcggctcatg gaagaccccc gtttcgcaga tcacaagctc
    65401 gtgttggtgg gggattacaa gggcgatgtg tttttttccc attatgcgga attgaaggcg
    65461 gacatcgaac agcgaggtct ctctgcccgc gtgctgttta cagggtttgt gcccgacagc
    65521 gaactcgcgg tgctctacaa cctcgctcgg gcgttggtgc tgccttctct ggaagaggga
    65581 ttcgggcttc ctgcggtaga agctatggcc tgcggagctc cggtgattgc cagcgaccgg
    65641 ggctctctgc cggaagtggt ggggcaggtc ggcttgtttt ttgatccgag tcgtccggat
    65701 gtgctggtga cgcttcttcg cgacgtgctg agcagcgagt cgatcaggga tcgtatgagc
    65761 ctggctgggc tcgaacgggc aaagttattt acgtgggagc tggcggcaca gtccacggca
    65821 gctctctttg aacgggtgct caatgaggtt cgttgatggg gccgtcgctg cggttctgca
    65881 tggtcacgac attttatccc ccctatagtt ttggcgggga cggcatgttt gtgtatcgct
    65941 tctccaatct gctggcggag cgaggccatt ccgtggacgt gattcattgc cgcgatgcct
    66001 attacgcgca gcatgccaca gagccggtcg gcgagtggcc aaaccaccct cgcattacgt
    66061 tgcacagcct caagagcaag gccggcatcc tgtcgccgct cctgacccag caattgggca
    66121 ttcccggttt gaaggccgga aagatcaagc ggattcttac cgagggccgg ttcgacgtga
    66181 tccattacca taatgtgtcg cttgtcggag ggccgaaagt cctcgaatat ggctgtgcga
    66241 tcaagctgta cacgacgcat gagtattggt tgctgtgtcc gacgcatgtg ctctttaagt
    66301 tcaaccagga ggcctgcacg aagccgagct gcctgatgtg ccagttgtcc tgtcggaagc
    66361 ccccgcaact ctggcgctac accggcataa tggatcgtgc gctcaggcag ctcgacgcgg
    66421 tgatctcgcc cagccaattt gccgccgatg cacacagaca tatggggctg aatgttgaga
    66481 agacggtgat accgatgttc gttcccagcc ggcaggcaga acaagagcgc ggcgaattct
    66541 tgcccgcttc cctgccggat ggtcagctgc catatttcct ctatgtcgga cgcttggaaa
    66601 agctcaaggg gctgcaagag gtaattccat tttttgcaca gcaagaccag gccgatcttc
    66661 tggtggcagg gaccggtcaa tatgagtctg agctaaaacg cctcgcccag gacagtccgc
    66721 gcgtgacatt tcttggggcc ctgccattcg agcggttacg gcaactatat gcccatgcgg
    66781 tcgcggtgat cgtgccgtcg ctctgctacg agacctttgg ccaggtggtc gcggaggcct
    66841 tctcgtttaa gacgcccgtg attgcgagag acattggcgc gttgtcggaa ctggtgacaa
    66901 cgagcggggg cgggctcctt taccggaatg gccatgaact gggcgatgcg atcgagtcgc
    66961 tgcgtacgaa tcctgagctg aaggcagagc tggggaaacg aggtcatgag gcctatcgga
    67021 agcagtggac cgaagaagag catctcgatc ggtatctggc gctcatcagt cggatcggcg
    67081 agcggaaacg ggccgaacat gccatggtat aaattttgct tcacaacgcg aaatgcttga
    67141 ttcgcaagca aggcggtcag cgctcatgag atatcgttgg aaaagccttc tggttaatct
    67201 ctcactcctt ctaggctctc tggcgttaag tctgatcttc tgcgaagcga ttctgcgatt
    67261 ggttgcccag aacgattcac cgctgtccat ggacatctat cagctcgatc cctacgacca
    67321 ggtgtcgctc aagccgaatg tcacccgtcg gcatttcacc cgcgagtgga atgtgactgt
    67381 cgccatcaat tccgagggac tccgtgaccg gccgagtccc gtgcccgaca cgcgaggtcg
    67441 catcctggca ctcggcgatt cgtttgcgtt tggctggggc gtcgaattgc cggagactta
    67501 tctctacctt gcggaagaac agttgcgatc ggaggccatc cgcgtgatca aggccggtat
    67561 tcccggcacc ggcaccaccg accaactaca ctggctgcgt caatacggcg gaacctatgc
    67621 gccgcgtctc gtgatcgtat cgcttttcgt cggcaacgac ttcacggacg tccagatggg
    67681 tgggttcaag aaacagttta ccatcaagga cggtttgatg gtcaggcgtt cgctggacgg
    67741 caccgagcgt agctcgccag ctcgagaagc gattgaaaaa ctcaagcgca cctccctcct
    67801 ggcccagcgg ttggcccagg tgctgtggta tttgcagaat acatttgttg cgccgcaaaa
    67861 tcgcagcaat cctggcctca acgctgtcga ccgctggctc tgggaatttg ccaaagtcca
    67921 cctgcgccag ccgcctcccg aaacggttga ggcctttgcc cgcacgcgga ctgccttgga
    67981 cgacatccat caatggtgtc ggcaaaacaa cgccgaaatg ctgctgctgg tccagccgag
    68041 gagcatgcag atttacgatt gggaactggc gaaatggaag gaggcttacc ggatcaacga
    68101 tgaggcgctg gacctcgaca ggccacagcg cttcctcgct gactgggccg ccgagaaaca
    68161 cgtggccatc ctggatttgt tgccggagtt tcgtgcgtac cagaaaagcc attctgccga
    68221 gcgattgtat ttctatccgg acagtcacat gaaccggatc ggtaatcagc tgaccggcgt
    68281 cctgcttgcg aagtggttga atgaatttgg attccagtga tggtgaacat ctactgcgct
    68341 ccatcgacgg gagagagacc tgggagccga acctccgcct ttcgaatgcg ccaggcgatt
    68401 cccagcacag ttctttaatt gtcgcggggg gcttgtccac cttgcatggc acgatcgcgg
    68461 gaccccggca acagtgaaat caactaccgc tgctcaaccg acaggggttc cacctggtcg
    68521 ccggaggaga atgtgtcgag gagtgacggc ttctcttcga cgacgctgct ggcttcaacg
    68581 gcacactatc ggcatatcat ctggctttac gatcgaattg gccgattcca ggtttagtac
    68641 gaatggcgcg tatttcgttg ccaagccacg agagaagata gacacttcgc gaggttttct
    68701 acggcgaaca gtcgaagccc atgaggctat gtctctacgg ctgaggagac tgcggatcgt
    68761 gggaagcaac ggtgcttctc cattgatcaa aagggccaga tgactccata ccggcttccc
    68821 atcctcacct accattcgct ggattccagc gggtctgtga tctcgactac tccatcaatc
    68881 tttcggcggc agatggagtg gctgcatcag caggggtttc agacgctgtc tctggcagag
    68941 gcggcgaggc tggtgcgaga tggacagccc tttcgtgaac ggacctgcgt ggtgacgttc
    69001 gatgacggct atgaaaccgt gtatcgggaa gcctttcctg ttctagaaga actcggtttt
    69061 acagcaacag tctttctgat taccggatat tgcggtaaac agaattcctg gcctggtcat
    69121 gtatctccgg tcggtgagca gttgttgctc tcctggacac agatcgagga aatggcgcgg
    69181 catcacatcg agtttggctc ccatacggtg acgcatcctg acttggcctg cgtgacgcgt
    69241 catgaagcgg cgcgggagat gcgagagtcg aaacagacga tccaggagcg attgggcggg
    69301 gacgtagagg tatttgccta tccctacggg aagtatgcct cgtgggtggt cgatctcgcg
    69361 aaggagcatt tcaccggggc ctgttcaacg atcttaggaa aagtcacgtc agaggcggat
    69421 tcggctctgc ttcccagggt ggatatgtat tacctctctt cgcagtggtt gatggaatcg
    69481 ctcgacacga gagggatgga tctgtactta ggcgtccggc gggcgatgcg ggcgaccaaa
    69541 gcatggtttg cgtgaaagta tagaaacagg aggtgaaagt tatgttgcag gttgaggagt
    69601 tctgttgccc aacgtgcaag ggggtactta gttttcaaga aagtgagtat gcttgctcgg
    69661 catgttccaa gaaataccct gtcatcctgg gcattcccga ttttcgtgtg ttccctgatc
    69721 cctatattga ttttgaagat gatcacaaga aggccgggca tttagccgag cagtaccaca
    69781 agctcgactt tatgggattg gtccgtctct attggaagat gacgccagaa gtgtcggagg
    69841 atcgcgccga acgattcatt agacgcacgg ccgctttagt tgaaaagggg gctgaatgtc
    69901 tgagcttgat tgagtcctta gctgagaccg agaagctccg cgggtttaat agagtactgg
    69961 agatcgggtg cggaaccggc ggatttctcg tggctgcaaa acgaaaatac gaccatgtag
    70021 ttggcgcaga tattgcgttt cgttggctgg tcgtggcgag gaaaaggctg gaagagctca
    70081 agatgaatgt acctttggtc tgctgctgtg ccgagtatct tccatttccg ggcacaagat
    70141 ttgacatcat cttagccgaa gatgtgctcg agcatgtgag aagtcaagaa agcactctgg
    70201 cggagtgtcg tcgcgtgatg acgaagcagg gaatgctctt cctggcaact cccaatcgtt
    70261 ttagtctgac cccggagcct catgtccgtg tgtggggagt gggattttta ccaagaaggt
    70321 ggatgaacgg ctacgtcaag ctcgtgaagg ggatttctta tgaaaatatc aaagtcttgt
    70381 cattcatgga attgagaaga ctgttgaata agtgcctgtt taccaaccat cgaattctgc
    70441 tcccgtctat ctcctgcgaa gaagcccagt acttttctcc ttttcagcgc atgcagcttg
    70501 ccatctacaa tgtggccaaa caaattcctg ttctacggtt gatcctgtat gtggtcggtc
    70561 cgtatttcaa cgttgtggtg ttttccagca gaaaataagc aagccaagac ggggagcgag
    70621 atgggtaaca agaattttct gtcagtcgtc gtccctgtat acaatggcgg ctggtgtctg
    70681 ggtcaatgtc tggcaggaat tcagcgctcg tcgtatcgtg agtatgagtt gatcgtcgtc
    70741 gacaatggat caacggacaa ttccgttgcg attgcgcaag cgcacggtgc tctggtggcg
    70801 cattgtccag gtccgagtgg ccccggcgcg gctcgtaaca caggagtgaa gttggcaacc
    70861 ggacagattc tcttgtttgt ggacgccgac gtggtggtgc ataacgatgt gctcaatcgg
    70921 gtggttgcaa ggtttatcga ggatactgaa ctggcagcgg tcttcgggtc atacgatgac
    70981 agcccgccgg ctaagaattt tctctcgcaa tacaaaaatc tgctgcatca ttatgtgcac
    71041 cagcaggcga ataaccgggc caccacgttt tgggggggat gtggggctat ccgtaaagat
    71101 gtctttgtga acgtcggtgg gtttgaccaa gagaaatatc ccacgccatc catcgaggat
    71161 attgagctgg gagaccgcat gcatcgcctg gggtatcgga tctggctgga caaaggtctt
    71221 caggcaactc atttgaagga gtggcggtgg aagtcgctct tacgcgcgga gatcctgtat
    71281 cgtgccattc cctggtctcg gcttattcac gagcggcagg gattggtcaa cgacctcaat
    71341 ttaaaattat cgcatcgagc cagtgccgca gtggcaggac tcttcctggc gatgctgccc
    71401 ttgaccccgg tttttccccc gctggcctac gggtgtcttt ttctagcaag catctttctg
    71461 cttctgaacc gtgacctgtt tgtttttttt ctccgccgaa ggggtgtgtt gttttcggcc
    71521 gtgacagttc cgatgcacgg gttctattac atctacagcg gcgtcacctt tgtcttggta
    71581 tggttgtcct acgcccttca cctcagatga ccggtggagg cactgtggta caggtgagtc
    71641 agtagttcat gtgagggaga aacgtttgat cacgagtatc catcgatatg gtgagtgggg
    71701 catcgtggcc ctttgcgcgg gcgtctacct tgctctatcc atcaccgccg ggctacggaa
    71761 acacagaaga gcatcgccgg acgatcacca gccagaagac tggcatgttt ctctctcgtg
    71821 gatacagcag cctggtgact tcttaagcgc atgctcattc tttttaacct gggtcagtgc
    71881 caatgcgaat gcgcaagggg accgatcggc ggtctcgccg ctggtcaagg aagcgtatcg
    71941 aaatgggata cctggcatcc ttgacggaat tgtgttcgta gagatctagc tgatcccgca
    72001 agggccgcgg cggtggttgg tcgctgcttg tccgctgtga ggcactaagt cagtgcctca
    72061 tccactggaa gatcaatgac aggaaaacct gactgagtcc aaattgtgac gtttcgccaa
    72121 ctcctacagc gtgttgctcc aaccgatgcc caggcaccct ctcgtttcga gagctgcatc
    72181 ctggtgctat ggtatgtgac ctgtgccaat gtccttgtcc tgttcctcat gcagggaggc
    72241 tacgatgtgc aagttggccc cattcatgtc cacgccactt ctctgagaaa ctggcttgcc
    72301 ctctgctttg cactcacgtt aagcaaagcc tggttggagg aaagacgggg cgccaggtcg
    72361 actttcgaag gaatgcagtc tccgctgctg ctattcctag gggcggtgac tgtgtattac
    72421 tccaatgggc ataccttcga aacgggcgat acgttccctg cacgttttct ccctgtaagc
    72481 ctcctcacgg accacgattt ctatcttgat gagtgggcct cgacgatcca tctatatgag
    72541 gatcaatact tcgtacggct tgtcaatggt cacctggttt caagctaccc accgtgggga
    72601 gccatccttg ccctgccggt gtatgttatt ccggtgctca aaggagctgg cgctgggatg
    72661 ttcgacctgg aaaaacgagc agctgtgctc atcatggcgt tgtcggtgct catcttgttt
    72721 ctcgcgttac gccgcgtgac gaggccgcgc gtagcatggt ttgtcgcgtt cgtctatgcc
    72781 ttcggaacga actgcctgag tcttaatagt caggccttgt ggcagcatgg gccgagccaa
    72841 ctctttctct cgctcacgct gtactgcttg gtgaggggcg tggaaactcc ggtctttagt
    72901 gcgtgggcgg ggcttccgct tggggttgcg gttatgtgtc gcccactgaa tcttgtcatg
    72961 gccgtgccaa tcgtactgta tattgtgcat aagcatcgtg gtcagtgtat tggtttcatg
    73021 attgccggtg ttcctcctct actattgttt ctgtggtaca acgcagtgca ctttggctca
    73081 cctcttcaga caggatttgg tgcgaccgtg gtcagtccct cttccttggt cggacaccat
    73141 ctttcctggt tcaacacacc cctgtttgaa gggcttgcag gcgtgctctt ttccccagca
    73201 cgcgggctgt tcatctactc cccgattttt ctttgtgcgc tggctggcat ggtggtggtc
    73261 tggagagaac cggaacaggt gcttctgaag tatctgagcc tggcgcccct tttcttgctc
    73321 gttcctgtgg caatgcttgg gagctggtgg ggagggcttt gctatggtcc aaggcttctg
    73381 gcggatagca ccccgttcct ctgtttctta ctgggccccg ccctcgaccg aattcaccag
    73441 agggtctggc tgaagtatgt agtggtgggg ttggcgggac tatcgctcgg catgcacgct
    73501 attggattcg cttattctgg cgattggaat tcggacttga ttgactttga caggcacccc
    73561 gaacgcgtct ggtattggcg agaaagccct cccgtcattt ttgccaaaca gctctgtacg
    73621 cagatgtggc agaaacttgc agcgctgctc tgacgaagtg ccagttcagg cgatggcgtt
    73681 caactcgcgc tgggtgttgc ccgacaagtc cgtgcccttg ggcaagtact ggcgcaataa
    73741 gcccttggtg ttctctttgg tgccgcgttg ccacgggcta tgcggatcgg ccggccagac
    73801 catcacggca gggttggtga gtgtggggtt gctcgtgttc ggcatcggag ccttcgtggt
    73861 ggtgcaacgc cagggcatgt tcgggtggat cctgaagatg ctgcggcgcc tcggcctgcg
    73921 tatccactat ttagaatcgc gtgagcaaca actgctcgac ttggatcgca ccatcgccgg
    73981 attttatgcg acgcagcgct ccgcctttct cctttcgacc gggctgtttt tcctggggtg
    74041 gctcgcggaa gcgctggaag tcttcgtcat gatcctctgt ctgggccaac cggtcacagt
    74101 gttgtcggcg ctggcaatcg gagccctcgc ggtgctgatc aaagggggca ccttctttat
    74161 cccgggtagc ttaggcgcac aggacgccgg caatctcttg ctgctgacgg ctttcggata
    74221 tggagatgtg accggaatca cctttgccct gttgcggcgc tttcgggaat tcgtctggat
    74281 tgccattggg ttggcgtgcc ttgccatgct cccaaagggc gaagagcctc cagttcctgc
    74341 cccctgatct gtattccggc cgtcgtccat ttcacacaaa aatacccgtc ctttttccta
    74401 cacc
//
LOCUS       DAORRQ010000016        72535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_18_length_72535_cov_62.215232, whole genome shotgun sequence.
ACCESSION   DAORRQ010000016 DAORRQ010000000
VERSION     DAORRQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 72535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72535
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18_length_72535_cov_62.215232"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PK224_07170"
     CDS             <1..450
                     /locus_tag="PK224_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRB15552.1"
                     /translation="AHLGLTKYFGIARPRIGVAALNPHAGEHGLFGNEESTSIAPAVK
                     LARAAGIKASDPLPADTLFGKAARGDYDGVVAMYHDQGLIPLKLVAFGTCVNLTVGLP
                     IIRTSVDHGTAYDIAGKGVAEHGSLLEAVKVAARLAESWSPVTKTSR"
     gene            471..791
                     /locus_tag="PK224_07175"
     CDS             471..791
                     /locus_tag="PK224_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15553.1"
                     /translation="MAETGLSAATMIDQQVKELDAIATRTIQDFNTTLGTERMGQWKI
                     RTVALLQQHAGQNAADELARKKPGMAFTNDLIEEFTDEVESYRSYLVTLAKRIRAAAP
                     PSAG"
     gene            808..1611
                     /gene="rsmA"
                     /locus_tag="PK224_07180"
     CDS             808..1611
                     /gene="rsmA"
                     /locus_tag="PK224_07180"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRB15554.1"
                     /translation="MASSLPPALKRLGQNFLIDPNIIRKIVSLAALGPEDTVLEIGPG
                     RGALTAGLCAEAGRVIAVEIDPQLQPHLQESLGHCRNLDLRIGDALAFPFETLPLRTV
                     VVANLPYYVSTPILFALLDARARLDRLVLMLQTEVALRLAAKPNSEDYGVLSVLTQEA
                     AEVELAFRVSANCFRPRPTVGSAVVHLRINTQEGFEPVRHERFRRLVRASFAHRRKTL
                     VNSLRDEGYPSEQIARATAEAGVPPQARAEMLTLDDYRTLARAFDRDPV"
     gene            1711..4146
                     /locus_tag="PK224_07185"
     CDS             1711..4146
                     /locus_tag="PK224_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRB15555.1"
                     /translation="MGVSTTAKRGDARSAPSRSSHVKREVIGVLLIAAGLLILLSLVS
                     FVPGDAKSIAASGAAGNQPKNMIGSVGALSAAACFFMVGGAAYLFPILLGLLGARCFT
                     PIPLTMRIRNAGSGLAAMVFLSALLHLEVTAVPTISSGWVNRGLAGGIIGQVLADGVR
                     SYFATTGAHIVILAGLMVALLFTVPLSLTALLQRVPDWWAAARERMAGLVPEWPTKQE
                     EAQPKRVREKKSRASREVEEQDFDREVQVVAEAVEQIPVPVIPPKIQPPMKVEKRAVS
                     ADEPAAAVATSPSVSDGYVLPDPQELLSDPSGPLARLSDDELKLQSEILTKALKSFAI
                     EGRVTEVRPGPVVTMYEFEPAPGTKVARIVNLADDLALALKAISLRIVAPLPGKSVVG
                     IEVPNPHREMVSMKEVVTSDAFSRSRSKLGLALGKDIFGGAVCADLRTMPHLLVAGAT
                     GAGKSVGLNTMLLSILFNARPDEVKLLLIDPKMLEFQSYDGIPHLLRPVITDPKSAAR
                     GLGWVVQEMERRYKLLADAGVRSIDAYNRRISEVQGAVSDVWQSGKPEQVELTFLSEE
                     ERLSKGEDAEPAGDNGPTDSVKPSPPEPLPYIMVMIDELADLMMVAPKDVEDKIARLA
                     QMARASGIHLVLATQRPSVDVLTGLIKANFPARIAFQVSSKTDSRTILDANGAEALLG
                     RGDMLYLASGTGKLMRIHGSYVSDDDVRRVVEFVKKQALPSYCRELQSLKIEEAEEEQ
                     AKDEVYEQAKDLVLSTGQASASLIQRRLRVGYPRAARMIEQMEAEGVVGAAGRDGRRE
                     VLGRRGPVGGDEA"
     gene            4143..4865
                     /locus_tag="PK224_07190"
     CDS             4143..4865
                     /locus_tag="PK224_07190"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRB15556.1"
                     /translation="MMRIFIIVAVLLMNGIPAVAQPVKPDEPAVDLQSLQEVQEVVKK
                     IQTRYEKTKDLQASFTQKTRIEGFSTPVISTGHFYIKKPGRLRWDYIEPATEEIYVNK
                     DDVKMYVPEHKQVLVGKLTYMAASQAPLQLLQGVAKLDEEFDIEPTANKDRGAGGILL
                     VSLTPKQGRAEPERAIQKIVIEVQPKTYFLKTIALHEVSGNIATFEFSELKPNSGLKD
                     DLFDFKAPADVEIVRAPVLSRP"
     gene            5105..5866
                     /locus_tag="PK224_07195"
     CDS             5105..5866
                     /locus_tag="PK224_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRB15557.1"
                     /translation="MSPTCTSSVTEAVERAIAALDLDRLEQEYWDQNEFLYIPEFLPR
                     DFVEATLAQQAQMLKSGLNRNYIPGHKKGGSVGYYAVMEKAPQFIDLYRSDAFRAMLN
                     RLTKVNLLLCPDNDPHSCALYYYTEAGDHIGYHYDTSYYKGARYTILMGLLDQSKQCR
                     LVCDLFKDVPGKQPVHRELATAPGDLVIFNGDKLWHAVTPLGEQEERIVLTMEYVTNP
                     DMGAFKRLYSNLKDSFAYFGLRSVFKRAYAPRSQS"
     gene            complement(5961..8693)
                     /locus_tag="PK224_07200"
     CDS             complement(5961..8693)
                     /locus_tag="PK224_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15558.1"
                     /translation="MRPPILIVDDDPDIRESLTDMLGHEGYLVHSAASGTDALQQAKQ
                     TRYDAALLDIQLPDLNGLSVLKVLMELDASLPVIILTGNVTPENTIGSLAKGAFAYLT
                     KPYNSQELKAILRRAVSMKGLAVRAEHVEEALQASEERFRALVESATDAMVLADQSGL
                     IIWWNGAAERMFGHTKEEALGRPLTIMMPERFRAAHAAGVTRLAQGGTATIIGHTIEM
                     VGLTKTGDEFPIELSLASWRASEGMFFSGIIRNIARRKRAEESVVRLSHQNALILDSA
                     GEGIFGLDPQGCATFVNATAARMLGWSAAELIGKPLAPLFYRIGNDNALYASDPFCLR
                     AALHDGAIHRIQNETFARKDGSLFPVQYVTSPIRERDRPVSAVVVFQDISARKQQEGL
                     QQAQLSVSHILAQAGTVEEAIPQLLPVAGTMGAWDMALLWQCGPMGDKLTCQGGWIRP
                     SHRWDEFLSVCRNSEFPPGMDFPGIAWASKLPLWISDVLADSRFTRAPTASRLGIHGA
                     CIVPIRTGSIIHGVLELYTEQRRASDSHVIQILADTGMKIGQFIDRTGAAQALRAAHH
                     MTQSLLASLPGAIFLCGSDWHVQYANALAHQYFAPPGESLIGRPLCECLSLSEPALQQ
                     LMLEWSTHTSDSPHRLSERECEVAARLYRYRFFPVSTPESPQYQLGILLWDITDDKQL
                     QDELIQAEKLSSLGTMVSGMAHEINNPAQAILSMAELIQDEDDPDTIKQFAADIVGYA
                     RHVSTVVRDFSGYARAAGRDGETEIDVAERLLEAVKMVRRGPHFGHVEIATQFEAPVF
                     LRARKGEVDQVFVNLISNAIQAMEGIGCLTLATGLDGRWIQVTIADTGPGIPDSIRPR
                     IFDPFFTTKTAGKGTGLGLSIVHKIVTKYAGTITVDSTEGGGTTFRLRFPALAQ"
     gene            complement(8933..11248)
                     /locus_tag="PK224_07205"
     CDS             complement(8933..11248)
                     /locus_tag="PK224_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15559.1"
                     /translation="MKGPTVKGPAHYLTSPSLYPSGPGRFISLRTKFVVFFSLIIILT
                     CSGMSWYFIHSKRIAMTERVQDLGTILVKNLAHNVRYGIIIQERVILEQFIDGVMGVD
                     EVVYALITGADGQILAAKSKGKLDSPLGTVRSAERPFYPRPDIADAVIKHSGTEPTIT
                     RLRLSGADTIPLREKDSTLPISTSGIHEETFYDFAIPVMKRSESRLEALALQEEETRK
                     AANAPTTPQVFGVVQIGLTEVPMQRELAKVLRNFLLLTLGIITAGILCTNLLARRIIT
                     PLRHLAAGARKVSEGDLSTFVVPTTQDEVGQLTALFNLMTKSLQERNQAISANLETIR
                     RHVTQLTTLNQSSTAITSTLDLDRLLSIVLQLLSENLGFARMVLFLWDSERGIATVGQ
                     MLGMPPEVEEAARRLEIPVCEDGGFAAELLIRGKPLFVPSIEPVADRISPAVLPWLRQ
                     VGISSFVAAPLRSQQRILGYLGADRGTHPCSQEDLDLLVTIASHVAVAVDNARAYTEL
                     ATLTDHLERRVQERTHELQAVNERLQEHDQRRSKFVSVASHELRTPMTSIKGFVENML
                     DGLTGQLSERQEHYLQRVKHNVDRLTRIINQLLDWSRLDVGRIEIKPESLHIDEFVRD
                     VVESFQTLAAEKSIVLEVLSCEQPITVRADRDKLEQILWNLVGNAIKFTPPSGHVSVS
                     CSICDARYAQITVADTGCGIAPDELPHVFNEFSKVESAMPSSQGAQLGLFITKSLVAL
                     HGGQMCVESRLGVGTQFYFTLPLDNSPASTP"
     gene            complement(11248..12216)
                     /locus_tag="PK224_07210"
     CDS             complement(11248..12216)
                     /locus_tag="PK224_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB15560.1"
                     /translation="MTFPFVQSPSTTGSADRFRRSLSWTVIALVCRLFSPGLASATDV
                     VILKSSDIAAYNQAISGFKAALPNDVVLAEYDLQGDLEKGRKLTRKIRAADPALVFAV
                     GLKAAKAAQLEIVDIPVVYAMVLDPAKYGLNAPNMTGVLMEIPMERQLIMIKSLLPRL
                     KHVGTLYDPSKTATLIEDARRLLKSNGTELVPIPLNNERDVPGALRTLLPSIDALWLV
                     PDSTVLTDESLRFILNAALEERVPVVGFSREFARSGALLCLSVNYGDIGRQAGQLTRK
                     ILDGHVSLPAKPLHPDRIEMSLNRKTAKFLDIEIPRDLEEKADELY"
     gene            complement(12274..15396)
                     /locus_tag="PK224_07215"
     CDS             complement(12274..15396)
                     /locus_tag="PK224_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15561.1"
                     /translation="MTAITPGHSILVVDDDPDIALGLQDLLDHDGYQVNVAGTCAEAL
                     TQAHEHQYNAVLLDLGLPDGDGSSVLRTLQEQQPQLPVIILTAYTSADRTVGSLTQGA
                     FAYLTKPYNRDELRAVLRRAIGIQALAAKAEHAEHALSESEARFRSLVEAATDSIVLA
                     DHNGHILSWNQASSRLFGYTDDEVRGQPLTMLMPQRYRVAHERGLARVKDTGQSRLIG
                     RLVELEGLRKDGSEFPIELSLAMWKTDVAVFFSGIIRDITQRRKAEEILDRLRHLHEV
                     ILTQAGEGIYGLDRDGLTTFVNQTAAQLLGYDPSELIDQSMHTRLHHSRPDGSAYPAD
                     HCPIYAALHDGVVHRVPRDVFWRKDGTSIPVEYVSTPIIEKGTVAGAVVVFRDITERL
                     EAELAVEESQERFLQLAEHIREVFWITDPAKTRVLYISPGYEEVWGRSCESLYAMPGS
                     WMDAIHSDDRLRVRDAAMSRQISGAYSEEYRILRPDGSVRWIWDRAFPIRDASGTVYR
                     IAGFAEDVTERKRVEAALIESERRYRALFDDNPSMYFMVDAAGIVLSVNRFGADRLGY
                     DVPELLGSSVLNIFHPDDQDDVRRNLAACLSEMGRPRGWEFRKVRKDGSVIWVRETAQ
                     AVLNDQQAPVVLIVCEDITAMKEAELALHDSEEFKNQILRSSADCIKVLDLEGRLQYM
                     NDAGQTILHIRDLNAYLNRPWVDLWEGDDRNAALAALEAAKAGEVGKFVGFCPSTDGI
                     AKWWDVQVTPMLDTRGYTRRLLAISRDITDYRQVQESLRASEERLESVIRGSNDGFWD
                     GHVLPDQPWHSPMTPVWWSPRVREMLGYNEQEFPDILDSWASRLHPDDRELVFDRLTD
                     CLEKGSSHYDVDYRLLTKQGDYCWFHARAEVLDRDAQGYALRMAGSLQCITERKQAEA
                     HLRLSEERLRRALTESEVGIWDWDLQSDRLYWSPDVEVLFGLTPGSFPGTYAAYIGLI
                     YALDRGSVLMQIEHSLHDQATLNIRHRVVWTDHSIHWLAWTGRIHRHADGVATRVLGI
                     VHAIKGHSDT"
     gene            complement(15465..17525)
                     /locus_tag="PK224_07220"
     CDS             complement(15465..17525)
                     /locus_tag="PK224_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB15562.1"
                     /translation="MTSFELNTAWYSLSAVLLLLCLSSVAAAEPPPVIDESMTPAVSN
                     DLELLKEEETVSIASRYEQPISQAPSNVYVITDEDIRQSGAIDLPTVLRRVPGLEIMQ
                     MTGGDFNVSARGGNQPFANKMLVMVDGRSIYGDVQGTVFWKSIPVTLPEIKRIEVLKG
                     PASAMYGFNAFDGVINIITKSPEEMKGTTLQFGGGELGTISSAAVHAGTIGKFGYRLS
                     IGRDQTQQWRDRDALGFRSNKFNVQTEYALSSTSKLQVSGGLVDTNRYDGQVGEVTSN
                     SIRPSLAYANVLYEQGAFLIRAWWSGYTDNATITPNSQLVDLLSITDRNGQSTNPFSG
                     NTYNVDVQHATNLWATHRLLYGATYRHNSLSSTTIDRFSREDRLGLFIQDEWRAASSL
                     TIVAGLRYDLHTQITGTWSPRVAILYQPVEGHTFHLSGSAAYRPPTLFESHQDQRVTT
                     TIPTGVPFPPSISSTVPISGSTRLAPEQIISYEAGYQGWYWKHRLRVRADLFFNHVSD
                     LIGSRITSGGASEFVNDQGSADIYGGEAGIEFLASRWLSGFANYAYEEIGQSFSGTVK
                     RGAPRSKVSAGLRTEWDNGLSGEISYYYVGATTYPIAQTFTTLASIPTTGVIAPGDRV
                     DSYNLLNLRVGYRFWQQKAAAGYMRDAEVAFSIFNALNDKHREHPLGDRIGSRSMGWV
                     TVRF"
     gene            complement(17533..19488)
                     /locus_tag="PK224_07225"
     CDS             complement(17533..19488)
                     /locus_tag="PK224_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB15563.1"
                     /translation="MICCCAIGAHAQDASPPATTPTAAAPVSEELELLKEEETVSIAT
                     RHEQPISQAPSNVYVITDEDIRHSGATDIPTILRRVPGLDVMQVTGADFNVSARGDNQ
                     LFANKILVMVDGRSIYIDGQGLVFWKSLPVTLPEIKRIEVLKGPASVVYGFNAFDGVI
                     NIITKSAQDMKGTTLQFGGGELGTISSSAVQAGSAGKLDYRLSIGHDQNQQWRNNDAL
                     AFRSNKFNVQTQYNFSGDATLRVAGGFIDTNRFDGPVSNVQIPRSQFNQGYTDVHYQY
                     RNFSVRGWWQGNDIPVDSATHPLLSQFVRQTNATGGSNVSFTTNTYNIEAQHSLEFGS
                     ANRLSYGVNYRYNTVDGSAVSELGREDRLGVHLQDEWKIRPSLTLVAGVRYDLHTRIN
                     GTWSPRVALVYNLTPEHTFRISGSVAYRPPTLFESNLDLRILPTVPPLFAPIPLLGSQ
                     KLNPEQIISYEAGYQGWWMKHRLRTRLDLFFNHLSDLINFQTVGTARTLVNGGEADIY
                     GTEAGLEFLATRWLSGFANVSYQKIGQTFTGVSRRGGPDWKANVGLRGDWDNGLNGEI
                     AVHYVASATYPLIEFFTALAPVLPSPTSPTVDSYTLLNLRGGYRFWHDKAEVAVAVYN
                     ALNDKHKEHPLGDTLGSRVMGWVTLRF"
     gene            complement(19845..20126)
                     /locus_tag="PK224_07230"
     CDS             complement(19845..20126)
                     /locus_tag="PK224_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15564.1"
                     /translation="MNRFIYLRSQKRQRMTVSLPTELLERMRDAAYWTSGTTMAGLIS
                     SALEDLLNNLESQNGRPFSPRLQDLKPGRPRANKTNRAPVSEDIQPVSN"
     gene            complement(20634..21326)
                     /locus_tag="PK224_07235"
     CDS             complement(20634..21326)
                     /locus_tag="PK224_07235"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HRB15565.1"
                     /translation="MAWPVSWHSAVGLSSTLPDGRAADLVTAPDSPMPLYKPRKNHAP
                     RARIGGQSRGAESGAPLLIALVPDHVAFTIKGDPSLCWYLSLPTTHAMTVTVVDSRGI
                     RPILERALPSPLHAGIHCTRPREYGIELKEQESYRWFITLVVDPDEPSRDIVAGGTIE
                     RIPYSEACSLGMPCTWTSCDRDAIYRYSESGLWYDAIACLLDLITYEGDKQSLERMLH
                     HLLKQSGVNLPT"
     gene            complement(21405..22112)
                     /locus_tag="PK224_07240"
     CDS             complement(21405..22112)
                     /locus_tag="PK224_07240"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HRB15566.1"
                     /translation="MAVKVGTTVLLAALLASSSEAGTDVQPEIPLYKAPQQVMPRARI
                     GGTLRGSEGEDPAIAALVPDHVGITVKQHPVLNWFLSKHTALPIKFTLIDERSIRALV
                     ERLITPPNQAGVHAVNLEDLGFTLQAHIQYRWYISVIKDENSPSRDIVTGGMIERCEF
                     SECMVLGATTTCTQEAVRTSAATGFWYDAMACLCDLITSDPGDTGLRKQRAALLKQVG
                     LHGVAEWDLAQTHAGQR"
     gene            complement(22219..24531)
                     /locus_tag="PK224_07245"
     CDS             complement(22219..24531)
                     /locus_tag="PK224_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRB15567.1"
                     /translation="MASRLRASLQPVLRVGLILLLSGTAASAAETGPSADELMQRGLS
                     AAERGALEQAHVDWKAAAQLYDQAGQVKGHLQALFHAAYAARALGHVNQAFLQQELAL
                     QLARRIGDPQWLALTLSELGKTYVTSHQYDTATDYLSQAAEIAHTRNLKTLSAALRND
                     LGIVLALQGRFPEALAAFQDSAAGATEENVRILFVRTRVNAARVAMQLKRFDEAAASL
                     NEADDRLAATAGSHNKADGLINLALGYRDLATVQPDEHARWLKRSAALLQEAAAMAED
                     SGGQRLASYAYGHLGHLYEEERRLDEALQLTRRAVFAAQSAGAPESLYRWQWQSGRLL
                     TRLGSLDGALSAYQEAAATLQPIRAEVASGSQASLDPAHQSIRSLYFELADLLLQRAA
                     LMTEDSEADSYLKAARDAIEAYKAAELRDYFRDDCVDQIQARLTKLDAVSPATAIIYP
                     IMFSDRTELLVSFPDGLRRHSVPVTASALTAEIRSFRRMLEKRTTREYLPHAQQLYDW
                     LLRPLLADLRRLKVNTLVFVPDGPLRTIPMSALHDGSQFLIAQYAVAMTPGLDLTDPR
                     PINRTRAQLLSSGLTKAVQGFPPLPHVAEEMAHLNSLFKGEQLLDSNFVTPRLEGELK
                     DGRYSILHIATHGQFATDVNQSFLLTFDDRLTMTQLERLVGLLRFRQDPLELLTLSAC
                     QTGIGDDRAALGLAGIAVKAGARSALATLWFINDEASSELVSEFYRQLHDSSISKAHA
                     LQIAQLKLLSGRLYEHPAYWSAFLLLNNWL"
     gene            complement(24562..26259)
                     /locus_tag="PK224_07250"
     CDS             complement(24562..26259)
                     /locus_tag="PK224_07250"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HRB15568.1"
                     /translation="MGASATAQIAPPLPPIFDPTGRSGKPPAPLREEFKAPETPPPSL
                     ILPEVSPAPHKPFENRLGSVRVFVHDIHFTGSTVFSEAELADVIAPYRNRELTTEDLE
                     RVRLALTLLYVNRGYLTSGAVIPDQDVTFGTIVIQIVEGTLTRIDVEGNQWFRSSYLR
                     DRVARGIDTPVSIQPLQERIQLLQQDRRIERINAELRPGDVRGESVLNLRVKDANPFK
                     AWLEFNNYQTPVVGAERGLATVAHQNVTGHGDQFVFTYGRSHGVNPIIDTSYSVPLNS
                     YETTLTAYYRRNAFLVVENPFRTLDLNVDSEVIGIAIRQPVYRTVSDEVALSLGGEHL
                     YLKTTSAFDVPGLPSLFIPGSSTTGVATVSALRFSQEYTHRSPSFVFAALSRFSVGLN
                     VLGATVNNGPLPDGQFVSWLGQAQAVKRIEEWWGIQLLGRVAAQIANDRLFPLEQMPV
                     GGRFSVRGYRENTLVRDDAVLTSFESRVPLLRFASGEDRLQFAQFVDFGRAWNAKGHT
                     PDPHTLASVGVGLRWTILPQERARFEVYWGQQLNHVRGGEGNMQDHGIHLQLVVQAL"
     gene            complement(26361..29933)
                     /locus_tag="PK224_07255"
     CDS             complement(26361..29933)
                     /locus_tag="PK224_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HRB15569.1"
                     /translation="MLSAFRIGSGHERHLLLGATLFASWLIQSSVPGIGHAQAPTPIT
                     SSGLNTTVTQNGNVYDITGGTRPGSGSNLFHSFGEFGVPTSNIANFLNETALPTSNIL
                     GRVTGGNPSHIFGTLQTTGFGGANLFLMNPAGIVFGPNAALNVGGSVSFTTADYLRLT
                     DGARFNAIAGPQDAAISSAPVAAFGFLGSNPAAIAVQGSQLAVAEGQGISLVGGNLTI
                     QGGTLADTTIQPARLSAPGGPINLIAVASAGEVLTNAAAAPADPTLNGFTSLGTISLT
                     QGADVHTSAHTAGRIVIRSGQFTMENARLEASATATAPTSPPSASATGGISVQADNVA
                     LSHSSNVLTSAVDGTAGNITFEVSTLRSNVGADGIPLSGAAPVTIASTSTGQGGAGSI
                     FFAGKAGEPADAVLLSHTHIVTGVTNAVDPTVVPGNIAMTAQRVELANGTAVRADTTG
                     GADAGAITLNVDTLKTQSGPDGRVLISSDSHCGNQCVGGQAGYITLQGIPGFTPPSTR
                     LYRHVTAPDSEPNRAITYYFAREFDLRGTDIHSDAIGNAPGGIVMMRSNGQASLIDSG
                     VSVTTQDFTINDNKPNGQSAQNQGFSRIDIMGRDIVLKDSTIKANAEVSDIGSCPLCQ
                     GGPSAGEIWLRAEHSFTADNSLIANTGHGRAQAGITKIIKDHYFSFGAVWDAPYPDAP
                     IDMVKLTNSEVTVEALHEGLPGYLRIRGDTTILDHSVVNSQVNNVTNVLDSQGRLIDV
                     VGAGEAGTVVTDGRSVQGSLLMSAKNLDITGGGIIAPTQGNRIANRIELHADVLTTRP
                     GTRAGGTLAAPRILDVADPSRVVISSSSGSTGGAGMISITGVSGPIPDDVPYPPSSTI
                     RLTGTDVLTDTSSIGLGGRIEMRARGPIELHNTNISANVTDLRPQSVNGQEQSGNIDL
                     SAGNILMEGGGISALTRGTRSGGNVSLSAAQSVTVSDGAAISASSTGLGNAGNITINS
                     GPRFLAQNASITTEANQASGGDIVIQAIDSIRFINGRLSTSVLGGPDTSGGNITLDPA
                     VVTLQNSHILAQAVQGQGGNITIIAGTFLADPTSVISASSQFGLSGAVNIQSPVSSLS
                     GSLATLPQQPLQAQHLLRQRCAAQAGGQLSSLVIAGRDALPAEPGGWLMSPLMFADEG
                     LTQSAQMPTFESAHQPPVRSEQPVDQFGPLPQLGAGDWPADCRS"
     gene            complement(29974..32931)
                     /locus_tag="PK224_07260"
     CDS             complement(29974..32931)
                     /locus_tag="PK224_07260"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HRB15570.1"
                     /translation="MKMMRGSRLFAGGATRLPCVCATMFACWWLIPLGLPDTSHAQVT
                     PPITSSGLNTHISKPSGNSLQYDITAGTRAGTNLFHSFGDFSVPTNNIANFLNDSGLA
                     TSNILGRVTGGNPSNIFGTLQTTGFGGANLFLMNPAGIVFGPNAALNVGGSVSFTTAD
                     YLRSTDGARFNAIAGPQDAAISSAPVAAFGFLGSTPEAITVQGAQLAVSEGQGISLVG
                     GNITVQGGTLAHTTIQPAQLSAPGGQITLVSVGSPGEVSATGIENQPLEPFVNGFSRF
                     GTISLSQGTTIDTSATKAGRIIIRGGQFTMDDASIKSITGNGHGMVTEGETASPAISI
                     TSETIALNNGTLITADTHGSAPAGDITFNVRTLTTEGDATNRLQLNPNDPSTIAQNLV
                     TSDSRSFATDAGSAGHITVQGVDGMGTAATSVTLKDTSLSTRIFGGSADSRPAAITLT
                     TKSLLLTNDDFPDAQGIGAATMVTTSLGPAPAGDIAINVDTLRVNVNHDETPIEGAHR
                     VFLNTPSVSAEDTAGPAGALTISGLRPESTDPASLIALNNAQLSSAVLGGTAALVPGV
                     IRITSDTVSMTGRTVFFAFTSRSAPGSNITFNVHTFRANVKQDGTLINGQPPSSIFSV
                     SGSDTGTAGKSGDITIAGLESASASAQLIAVNNTEFTTTVSGGTAATNPATITLSADS
                     LSMNNSRIRTDTNGGAPAGSIVIDTVRFQADQQTKISSTSSSEGTGGHISMAASESVT
                     IRGGSNMTAESTGPGNAGNIVINAGNQFLSQNGSITTQASQASGGNITLQAIESIRLV
                     NSQLNTSVQGGPMTSGGNITLDPAVVTLQNSQVLAQAVQGAGGNINIIAGTFLADSAS
                     VVSASSQFGLSGAVNIQSPVSSLSGTLATLTQRPLQAQQLLTQRCAAQAQGRLSSLVV
                     TGRDALPSEPGGWLMSPMAFMVPEVPAQEAPQVSGFSFGPEQSQRLAGQMSHHLGSAS
                     RRGVWDRETGCGS"
     gene            complement(34127..35515)
                     /locus_tag="PK224_07265"
     CDS             complement(34127..35515)
                     /locus_tag="PK224_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRB15571.1"
                     /translation="MPLAQRFRRALLLCLLCSAAGFVFAAASANAQTPRLQGQSAAAA
                     AMGNAFVAQADDPSALHYNPAGMTQLHGFQTLFGTTLIGGTTQFTSPTGAQVTGDRNG
                     SVAWPPPGHVYLVANLKDLGLSALGNFTAGIGMNNPFGSLTRYPNDGPFRTAITFTTL
                     PLLDIKPTIAYKLNDQLSFGLGADIYTFSGLFGEGHLEQKSIWPGGLGIPAGSLMEIN
                     GSDTTAGFNVSLLYTPFRNSDGKPLVNIGMVYRSQATFHLTGNFLANGASVAGATATF
                     VLPQVFSGGIGIWPVRTSEREWKLEFDVDYTGWKSNRNLDVHLTNGALLPFPQNWQST
                     YTVMIGTEYRWLRLASMPDWDIALRAGYMNQQAQIPDRTFNPGVPSANTHIPSVGIGF
                     ACHENGSFLGLVRCGELGIGPLKPKLFAIDLAYQAGLYEVRTIAGNQNPTVNGRYDSM
                     VHVGSLSLRFNY"
     gene            complement(35520..38237)
                     /locus_tag="PK224_07270"
     CDS             complement(35520..38237)
                     /locus_tag="PK224_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15572.1"
                     /translation="MTDLLSKEHSILERPFMEFRPFGLNDQGEKICDISGVIVQSNVD
                     YMCDYLSRTAGKAAATQALDELCRLLNARLPDRTYHVTPDSLRNIWNSYSYEFVCYLR
                     EFCEQLSGDPQFHVNVGTNRKVPPLIQILCRPFSTPQLYKMWPYFGSKYVRGVLEFEV
                     GRVTRRSAVLRMTFTDRALVQFGPYRKRCVDVICLSCKSSIARVQTQMHGTPAATVRD
                     LSCTANGDPYCEWEFTWTPQVRSSVPLAMWMLVAGGTFTYLHLRQPDVPTVEALGLAA
                     IPASLLWWMITNHMRQAAKPLQRLIKDQEQVVDARHEELREAYLEQQSTAVTLRRKIN
                     DLTTLHRAGLLFSSTFDREELLTNVLETIVRELHYDRAMITQFDRTRNVSHDFRVRGM
                     PQEVADFLRTQEVPVSDPNSVEGTVFLRGEPVLTNNIHEIWDRLHPFDQKLIAMVNVK
                     SFITVPLKTQDAVIGSLSVDRTHNQALTQDDLDVLVTLASQVAIALDNAQAYREIESL
                     NAGLEARVRERTAELEAANAQLKQMDRLKSKFLAHVSHELRTPLTSIVGFADNMLEGL
                     VGSLNMKQEQYLTRIKANGTRLARMITDLLDLSRVEAGKLVLSFEQVCLPTIVSDVIE
                     QLLPLAITKHLNIELHSPDPELLVWADQDRLSQILTNLLDNAIKYTPDGGQVSVELSV
                     DTHDMARIVIRDNGQGIPPDALPKLFDPFFRVQQEERSQTKGLGLGLAIVKDLVDLHG
                     GSITVRSEVDQGTEFTFTLPLHSREPSPAGQLPAVRRTVLVVDDDPDICDLLRDRLEA
                     EGVHVCTAADGRAALRMLAETTVDGVLLDIALPELDGFEVLRQLRPTHPTLPVVMMTA
                     VEALDRAMAAVESGAQDYLLKPFDGTRLRQIVDRWFVIGSGQSESPIGR"
     gene            complement(38382..40115)
                     /locus_tag="PK224_07275"
     CDS             complement(38382..40115)
                     /locus_tag="PK224_07275"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HRB15573.1"
                     /translation="MPFYSQRIVVLAGLLFALSTVHCQAIDLPPPHTVSSSDTQGWTE
                     QDRQQWYHTSAGTQLLPYDWFVVLEDEPLKNSFARTGIIPDQTHPDRLPIGFTKTEGP
                     NVPEPTVGLTCAFCHTTQFTYQGNPIRIEGGPSLQYNQRFLQVLLESLGELKAPDKFQ
                     AFAARVLQRRGQAVTQENIATLAGQFSQVMKDLVARGGRDASPALWGPGRFDALGRGG
                     NTVFSPLNPDNLRPANAPVSIPPLWGVWEYDWVQWAGSIQHPLARNIAQVIGVNAALF
                     SWARTSPQPPSEKKEIFRSSIDVASLKTLEGLAKRLHPPRWPKSFPPINRELAARGKD
                     LYHGNKLKGLPNLCAHCHVATKLDSPNPNGPSLHVTMIPQKEVGTDSLYLENFSRRTV
                     DLERLGLGRLSAKDASQLVTTELMTLNGAGSDPEYQHLTNTWRDKAQYIARPHLAVWA
                     TAPFLHNGSVPNLYALLSPVKERPACFYLSPNMEFDPVKVGFVVSECNDSPTFRDPLV
                     GFEFKTHLPGNSMEGHEFKGSDCGSVVAGAGVLGCEIPIADRWAIVEYLKTCDLDRLV
                     IHDAPACRDLE"
     gene            complement(40468..40983)
                     /locus_tag="PK224_07280"
     CDS             complement(40468..40983)
                     /locus_tag="PK224_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HRB15574.1"
                     /translation="MDSVQSIFLDLNQLIPIVNHWLHLLSAVIWIGGLAFIVMAVKPG
                     LREAALPKEYVRPITDAFYKHYKRVAGILLVVLLFTGGINLHYVNQIFVSQTGQGMQN
                     HPKYLWIFCIKLILVLCLLTLFLYTVIFKSDQDEEEEGENYESIPFQRAALWMGFFII
                     LCAAAMKHLHQ"
     gene            complement(41054..42457)
                     /locus_tag="PK224_07285"
     CDS             complement(41054..42457)
                     /locus_tag="PK224_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB15575.1"
                     /translation="MGKSLPVLNFPFLGDKLESLQQQISKFISPTSPSAAPNDGRTVD
                     DFKPYLVALNLTKRCNLKCDHCYLDATTKAGGGSDELSTEECFRLIDQIAEVNKGCLL
                     VITGGEPLVRPDILDIARHAVGLGFIVVFGTNGMLINDRMAKTLVEIGVMGVGISIDS
                     LDPDKHDQFRGVPGAFASALAGIEACKRNGLQFQVHFSAQPMNYQELPAVVDWAHTLG
                     ARVLNVFFMVCTGRGEELTDITPQQYEEVLGYLVDCQDQYKDMLVRARCAPHFKRLAY
                     EKDPNSPLTKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNVRETSFVDLWER
                     SDVFNSFRYPQLKGKCGDCEYTDICGGCRARPYVDHGDWLDEDQWCLYTPKGGDKIKV
                     AFNTPEETDIAWDEASALRLSRIPYFLRAMVKKGVERHARETGIAMVTVELMEELRKK
                     RFGNDAPVFNFDMRGKD"
     gene            complement(42457..44568)
                     /locus_tag="PK224_07290"
     CDS             complement(42457..44568)
                     /locus_tag="PK224_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB15576.1"
                     /translation="MYKTIYIPVDNSDHSNTAVDVGVELAKTYGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDYVDKKCNEANLPV
                     ERRSLEGRNWKVLAEDINTNAYDLVIMGALGVGAVKDSVIGSNTERVLRRVRNSDMLI
                     IKQPTPVGTGKIVVAVDGSPYSFGGLMTGLALGKAFNVPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEVIDSGLAKIYQSHLDISREIAQAEQTDVKTTLLDG
                     KAFEKIIQYVRKENPWLLIVGRIGVHSDEDMDIGSNTENLLRAASCNILVSNRKYVPP
                     IDTQAEYTIAWTEEALRRMERIPVFARGVAKTAIHRYAIEKGHTIISNTVVDSAVGHI
                     LPKGAMDAMRALGGNLDAAGIDRDKMQADDSVAQDLMGSTLSGMMTQVVEEKPVNSPG
                     TQAYLDRMSQNYFVCDGCGYIGKGDTPVKCPVCSAEGDRFKQVDKTIFEAAAKAEGGL
                     ETDLAYDDVPMQWTKDAKEAIRAVPAGFQRRRAKAKIEKSARKLGMTTITLEYAGPMI
                     QEAATEDYTPIFANKGTGTTPQLAPAAAETNGADAHAKNGHGEEPAPVPQETVSPYTW
                     TPDAQGRLDRAPEGFMRECTKALIEKHADKIGTTVITLEVATEGIEQAKGYMADAMKT
                     GNLKDMIANLTGSSSKTGANR"
     gene            complement(44733..46388)
                     /locus_tag="PK224_07295"
     CDS             complement(44733..46388)
                     /locus_tag="PK224_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB15577.1"
                     /translation="MESILYIFLPCKKVYPIGVTYLADFIHRRKPEVRQRILDLSLFS
                     EPQRSQAIRDAALEFKPDLVCFSWRDIQIFSPHEGDASLEHAFNFYFAGNPIKRVAAS
                     FAGLKQLYRYYSHIRANLSYPWLIRKEFPKTQIMIGGGAFTAFADQLIEKLPEGTIGI
                     LGEGEDAILKVVNGESLENERYIIREGKQTRKGNQGSPALLDALTVDLPYLTSIFPQH
                     AAYMDESIGVQTKRGCPYDCAFCLYPYIEGKRVRYRPPEMVVKDISQHYHQWGARRFW
                     FTDAQFITGKEAYPQCTEILERILSEKLEIEWSGYIRTSLITPDLAKLMVRSGVGDLE
                     VAITSGSQEVLNNLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEESLLQS
                     VESYKKVAAILGEERVFPLMFFLGIQPNTDLEHRLLEEGYLSAGYNPLMLTPTSIRKL
                     LYNPAPLNKIIAKACLAAWERKEGSRDPRAWTGSLSQTTTQATASGPKSDTAHYADAN
                     LIRGIQNNSGRDALLTLEEILRSRRPAQPTTATTEKTAVSPIS"
     gene            complement(46484..47266)
                     /locus_tag="PK224_07300"
     CDS             complement(46484..47266)
                     /locus_tag="PK224_07300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB15578.1"
                     /translation="MLKVQEQPTPATDRPSPQPQAVSTWSGQAREQAVQRMFTAIAGV
                     YDLNNTLLSFGLHHHWKRLTASYVPTVRNGRALDIGAGTADLALLIEPKMGEQGYVIA
                     SDLNHAMLAEGLRKVTGRGLRDRITCLQANAEQLGFPDETFHAVTTGFCMRNVGNLTQ
                     AFTEIRRVLRPGGRFVCLEFSRPAYGWLRGLYDWYSFRLLPWIGTKVARDRTGVYEYL
                     PASIRTFPDQERLATLLREAGFRQVEYRNLTGGIVAIHIATK"
     gene            complement(47571..47837)
                     /locus_tag="PK224_07305"
     CDS             complement(47571..47837)
                     /locus_tag="PK224_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15579.1"
                     /translation="MARELPLRSMPKEYRPVDWSWVVAGVRYQFPRIFLLRGFQEKPE
                     ETGATDIFRGSEIPIDRQVIGVMVIFLGQASSQMLGAARSQVSL"
     gene            48158..50875
                     /locus_tag="PK224_07310"
     CDS             48158..50875
                     /locus_tag="PK224_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRB15580.1"
                     /translation="MKGELADLARWTDVESQLVERIEKLLLGTTTGDDGGGERRTYPL
                     TRAALFAAYEALVQELGLPTEWVEPPEFAIRSYVYFKDSNPPEPLLLNSFFLPDLGTA
                     RKQFTEAKAPKNLKRYLGVERPQNRIDLLNNRPALAEAISPGLTGPSRWPGAGRSPLV
                     LLQQAAVNLAFQETKAGGLLGINGPPGTGKTTLLRDLVAGVVTERAEAMAKFDDPEAA
                     FEHSGEKLRAGASWIHLYRLNPTLRGFEMVVASSNNKAVENVSAELPNLSAIAEDAPE
                     LRYFKTLSDAFQPAEPSETWGVIAAVLGNMQNRSRFKQVFWWDEDNGLNAYLRAATGL
                     DSDVKITDPVTGKAHQRPAHIVQQEQPPSSREEALERWRGARSRFLHVLDESRRRQAW
                     LESLCAELKRLPELAQNEMNVRSQRDAASEKVRQLKGLSAEAWEVAHSSQRAAIELQL
                     HDLSMPGFWARLFRTRIARKWNEIRRALLEWQNAEVAWQVACAQHQQANQRLVEAQQK
                     HGVILTDDVFFSLEHSKRHQATPWFSPATQRLRDEVFISAIAMHRAFIDAAAKPLRHN
                     LGALMDVFTTQTLKGEEKQALLSDLWASFFLVVPLISTTFSSVNRMLGKLPLESLGWL
                     FVDEAGQALPQAAVGALLRTRRAIIVGDPVQIEPVVMLPDTLTHAICRRFGVDPNHYA
                     APSASVQTLADSASSYTSEFQTHVGSRNVGVPLLVHRRCSEPMFSVSNRIAYSGLMVS
                     AKLPKPSAIREILGPSAWIHVEGSGQDKWCREEGDEVIRMLHRLRAASAKPDLYVITP
                     FVIVADRLRQTIRESGVLGGWVSEDEWRWVHARIGTVHTAQGREAEAVIFVLGAPHPT
                     QTGARGWAGGRPNLLNVAVTRAKEAVYVIGNRQLWREAGLFRELDGKLP"
     gene            51041..51340
                     /locus_tag="PK224_07315"
     CDS             51041..51340
                     /locus_tag="PK224_07315"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRB15581.1"
                     /translation="MKNTRDEVLKVLEANRHALHAYGVRSLGLFGSVARGDATPASDL
                     DFVVQFDKKSFDGYMNVKAFLESLFGCRVDLVLESTIKPRLRRTILGEAVYAAGL"
     gene            51324..51662
                     /locus_tag="PK224_07320"
     CDS             51324..51662
                     /locus_tag="PK224_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRB15582.1"
                     /translation="MPRDYKVYADDIIGAIEKIKRFTVGLDRETFSQDEKTFDAVIRN
                     LEVIGEAVKKIPEEVRTRYPLVEWKKIAGVRDILVHEYFGIDVEIVWDILQQKLSPLE
                     SQMRQILADH"
     gene            complement(51722..52486)
                     /locus_tag="PK224_07325"
     CDS             complement(51722..52486)
                     /locus_tag="PK224_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB15583.1"
                     /translation="MYNQDGHQWIVIGRDPEKDSHVIDTNEYVIINRGHAMLLDPGGI
                     QIFPQVLAELAKYVRMQDIRVIFASHQDPDICSSLAMWLDLNPAIKTYCSWLWTGFVS
                     HFSTGEVITLNPIPDQGMRIRIGDEGPEVEAVPAHYCHSSGNFSLYDPTAGILFSGDI
                     GGALVPDHHASLVVTDFEQHIQYMRGFHLRWMPSTVALRGWTQRVRAIKPRMICPQHG
                     SIFEGEMVGKLLDWLDSLEVGQWKDSAAKTDTRAAA"
     gene            complement(52491..53282)
                     /locus_tag="PK224_07330"
     CDS             complement(52491..53282)
                     /locus_tag="PK224_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15584.1"
                     /translation="MHDVLSLFKRNINQLFGITVDILNVGRSLQQLSLSMQILANNGV
                     VQAAKIPGGKGRPMLALVEILNNTPKEIRPEVEALEHLCAGLAKVTASSSNIVWRYHQ
                     LIASLLSAMAHAEPTSTSKHLETLSHLRFTTAADVAQLMHHPAFSDAEALERDNRAYI
                     VHLCQANLAELHERLKEALRCLGETQQALVGLKTIGLTARYMAFCIASEAAGLAEAEA
                     NFKNLATEITHVVDDLDAKTRAMKEAIEHGQELLELLLKGHTYAK"
     gene            53488..55995
                     /gene="uvrA"
                     /locus_tag="PK224_07335"
     CDS             53488..55995
                     /gene="uvrA"
                     /locus_tag="PK224_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRB15585.1"
                     /translation="MSNSIIIKGAREHNLKNLDVEIPRDKLVVMTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIYDYLRLLYARVGHPYCFQCGQEITAQTVQQMVDAICELPEGTKFQILSPIVRGRK
                     GEYRRELLEMRKAGYVRARINGEIVDLGEDITLDKQKKHNIEIVVDRLVMKPGDALMR
                     RVADSVETSMKLAGGLVGVLSETGKVHLYSEKLACIKCGVSYPEITPRIFSFNSPHGA
                     CPACDGIGYAMTPGASEEEDFTLLERCETCDGARLKPESLAVKIAKKSIAEVTMLSVR
                     AAADFFTSLKFTERELVIAHRILKEIRERLGFLVNVGLDYLTLDRPAATLSGGEGQRI
                     RLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLRLRDLGNTVIVVEHDAETMEA
                     ADYILDLGPGAGTHGGRIIAQGTPKQVMANPDSLTGMYLRGEQMVSLPPRARKPKGFV
                     TVVGAKKHNLKGVTVNIPLGLLTCVTGVSGSGKSTLVLEVLFHSLSQLLYHKKPKIDG
                     CKELKGVDALDKIIDIDQSPIGRTPRSNPATYTGLFTFIRDLFSNLPESRVRGYKPGR
                     YSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQRYNRETLEIQYKGRSIADI
                     LNMTVDDALEFFENIPYIKTKLQTLHDVGLHYVKLGQSATTLSGGEAQRVKLSRELSK
                     RPTGRTMYILDEPTTGLHFADIQRLLDVLDRLVETGNTVLVIEHNLDMIRNADWIIDL
                     GPEGGDRGGEIVAEGPPKEIAKVKRSYTGQVLRDAGV"
     gene            56263..56946
                     /locus_tag="PK224_07340"
     CDS             56263..56946
                     /locus_tag="PK224_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15586.1"
                     /translation="MSTAETHREIISIIGQEYFTPIAHLIDKWVDSRPVRRDSVGAPF
                     FEGGYAVSVIILLVAAVESFVARDRYFSKRQPYGNHAAVPEYMKEMYRYRGYKRLSEL
                     FVVRDAVIHSHVWALKFAIRKSGSRRFVSASRISWSGNNRLKSRLNPKTYRTKLLRFN
                     TIPSRIDRTDVIKAFGIVLAAIRFLTTSGAYPVPVFPFRVQHQGERMSFEELPNCLAK
                     LLPPSTGRE"
     gene            57005..57334
                     /locus_tag="PK224_07345"
     CDS             57005..57334
                     /locus_tag="PK224_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRB15587.1"
                     /translation="MHTILLLTISNIFMTFAWYGHLKYKESPLWLAILASWGIAFVEY
                     CFQVPANRIGHYEFTAAQLKTIQEVITLIVFCVFSVLYLKEDLKWNYLVGFGMMIGAV
                     FFVFKEW"
     gene            57607..58035
                     /locus_tag="PK224_07350"
     CDS             57607..58035
                     /locus_tag="PK224_07350"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HRB15588.1"
                     /translation="MKTATSMKNLGLGLCVVAAGFMVGGCNTTKATVDTTVNFFSSTS
                     PNGLFSADGMVLKEQKINLFAGVAYENLRQEAAAGGGQYVSALASLYEIPSGKQEDFG
                     LVLQRKHADLFAAGLEDDRTAHLKMVSALNRELVAASLLP"
     gene            complement(58292..59206)
                     /locus_tag="PK224_07355"
     CDS             complement(58292..59206)
                     /locus_tag="PK224_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15589.1"
                     /translation="MYRRWILSLVIAVCAVPGLALAEAPPHSVHWGAIAFPDHDPTLT
                     LSAALLDRFTEFDGEGRRYNDMRETMGLNFFTLSWTKPLAQLPGWNLNLTAGGGPTRD
                     GPSRFLQNDVVHKFRGLTEVPVGNKREANDFMLSGSLTRWFSLLGSNDAFFAGLGGAG
                     GSLYYEPYVQAGFRRLALFAPVPLLGDYLRVSALARYGRPFSGAAFHQVAPQSYMAQG
                     SVGLGNYRHWADSTPWEIELAVTVDSGLFVDHRGDALEERFVSVAVRYSAFTFETWND
                     LINQKDYGPTFGARLTLDLLYMYDRWFK"
     gene            59384..61882
                     /locus_tag="PK224_07360"
     CDS             59384..61882
                     /locus_tag="PK224_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15590.1"
                     /translation="MPPKKKQTTTGTGSSTDDAAEFEKLGVFYLGRPYDLAAKQAKPG
                     WLLYDSKDLVTHAVCVGMTGSGKTGLCVGLLEEAAIDGIPAIVIDPKGDLANLLLTFP
                     QLRGEDFAPWINEDDARKKGLSAADFAAQQATLWQKGLGDWGQSGERIKKLRDTADFA
                     VYTPGSNAGLPVSILKSFAAPSAELLDDAELLRERVGTTVTSLLGLIGVEADPIKSRE
                     HILLSSILDSAWRAGRDLDLPALIHQIQTPPMTKVGVLDVESFFPSKERFALAMQMNN
                     LLAAPGFAAWMEGEALDVGRMLYGPSGKPRIAIFSIAHLTDAERMFFVSLLLNQTLGW
                     VRGQSGTTSLRAILYMDEIFGYFPPVANPPSKAPLLTLLKQARAFGLGVVLATQNPVD
                     LDYKGLANTGTWFIGRLQTERDKARVMEGLEGAAASSGKKFDRRQMEQILAGLGNRVF
                     LLNNVHEDAPEVFQTRWTLSYLRGPLTRTQIKQLMDPQKRETSNVKRHASQDESFRST
                     NGESRMTPTRPMLPPEVPQYFVPLRGRQPEGHALVYQPMVLGVAQVRVVDNKAAVDVT
                     DPLTLLTPLTEGAVPVEWDHATIVDLAVADLERDPAGGAQFATLPAAAGKAKQFDGWK
                     NDLSGWLFRTQKVELFRSPSTKALSMPGEPERDFRVRLQQAGREQRDQQSEVLRKKYS
                     PKIVALQDRIRRAEQMVERQQAESRSSQIQAAISVGATILGAFLGRKAISAGNIGKAT
                     TAIRGAGRAMKESKDVSQAEDNVAALQQQLAALEAEFKAEADALAAATDPLTEKLETL
                     SLKPSKSNISIKLVALAWVPHWRDESGTETPAWQ"
     gene            complement(62042..66004)
                     /locus_tag="PK224_07365"
     CDS             complement(62042..66004)
                     /locus_tag="PK224_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB15591.1"
                     /translation="MTTPASPDDPLTLATILEEETELLHGPLPKDHPVGAPDAVRTAA
                     LFRHIHACHPKRAGLCFSGGGIRSATFGLGVLQSLARLQLLNKFDYLSTVSGGGYIGS
                     WLTAWIHRHPQGLDGVIEDLRVTPKTGATEVPPPVQWLRNYSNYLSPHLGFLSADSWT
                     LFGIYLRNLHLNWMVLLPLLMVPLLVPRWTIALAQLNTPGRTLPVWLLQAVFMIGLGL
                     AVMALIYLHLCRPTLREYRRNTRWQTLERQHWFLVACLGPLITSVLFLTTAWAWFRNG
                     GGTLEQLSLPHAILGGVFLHTGSWLFSVLALKRFKAFSPWLFWETAAVAATGALGGLF
                     LRSILMKTPDQLVVATFAECFATFAVPGVLAIFLLTATVFIGLASRFTEEQDREWWGR
                     TGSWVLIVMVIWSGLSAIVAFGPGLIAWTPKIAASLGGLSGLLTLVLGFGSRTTAQQQ
                     EERSHTTVITDFAVRAAAPFFMLCVLIALSLGTSWQLDLFAHHYEMHLNHLTGPMQTG
                     LGIWPDPWGHDQVLHNTPLWMLFLFTVAVTLLGLLMSGLININRFSLHAMYRNRLIRA
                     YLGASRIQTERERSFNPFTGFDNLDNPAMSDVRFDDVRLARRIAREVFSTDTRAQLDA
                     FHALQEPTSAQLQAMIAQLTQELSHSLTNKALAKAAEQLRVGPACRAKIAALQQPETP
                     AGRDAITWLTTLFLNKLHDQSPRPLHLINIALNLVKGDNLAWQQRKAQSFTISALHSG
                     SWNLGYRRSEEYGCNHYLAQPLSLGTALAISGAAASPNMGYHSSSAVTFLLALFNIRL
                     GWWLGNPGRAGADTYRKASPNVSVGPLLSEAFGLTDSCHPYVYLSDGGHFENLGLYEM
                     VLRRCHCILVVDAGCDPQLTFEDLGNAIRKIRIDLGIDIELNLEQIKRAADAKTSDRH
                     HAIGMIRYDKVDANATAGTLIYLKPSLTGNEPSDVQDYAARHPSFPHEPTSDQFFDEA
                     QFESYRRLGEHIAQQVLRPAIQRSEQDFSSLCHALRSYWLTTPPGMRESFLGETDDLK
                     DLERLLRDDPDLLRYDLEIYPELRSVFGIDPGAIAVNPRAALHTCNLQIQLMEQVYLA
                     VNLEEFHNHALNRGWMNLFRRWTSAETFRLFWPTLCGMYSQQFVRFAEQHLNLSIDEV
                     VVLEAMGPTSDLSSLVRDLSIEWDSVPDYAETFMQALHKPLPLEEPTGDHTRRAAWHM
                     QLKARPSPDDSAGPGEILAVVAATRLSVAGHRLALHGWVRPAYRGLGLGHRLFRHAIE
                     ELTAAYSGHNLIVDLGPDNPAWAGTSIRNVGWLRFYEQLGFTRDRSDPTRFRMSRQLS
                     "
     gene            complement(66274..66831)
                     /locus_tag="PK224_07370"
     CDS             complement(66274..66831)
                     /locus_tag="PK224_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRB15592.1"
                     /translation="MSHPSAPHTPSPPSTAAHRVEQLFEILIFGSRWIQAPLYGGLII
                     AELLYAAKFLTELWHMIVHFREETETLFMLGVLSLIDVTMVANLLTMVIIGGYATFVS
                     KLDLETHPDRPEWLTHVDPGTIKIKLAASLVGISSIHLLKAFVDVTNENPEHIKWKIF
                     IHMTFLGSAIFLAYTDKLMQRDRKH"
     gene            complement(66924..69782)
                     /locus_tag="PK224_07375"
     CDS             complement(66924..69782)
                     /locus_tag="PK224_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15593.1"
                     /translation="MAVHHLPIRRRQDNALDPSILNVLPANIAVLDAKGTILAVNESW
                     ERFAAANGFTGEGYGVGRNYLHICDAAGPQPDAQHVAQGIRHILDGTIRAFDYEYESS
                     SPTLRRWFRVIVTPKQGSPSYGAVVMHINVSDLKQAEAAMHQSEARLRAIVDTAVDGI
                     VVINERGIIESLNPAVTRMFGYTPDQLLGQNVKLLMPDPYHTEHDRYLTNYLQSGQKK
                     VIGIGREVLGLRHDGVSFPIELAVSEMLVDGVRKFTGIVRDISSRKQAEAQFRQVVES
                     APNGMLMIDPLGNITLVNKQVETMFGYSREEMLGKPVEMLIPERFCPDHPAHRIGYFS
                     APSSRAMGAGRELFGLHKNGQEFPVELGLNPIDTPNGKLALASIVDITMRKRTESALS
                     KAAQDLERKNWEISEARDQAVRAGQAKTDFLATMSHEIRTPMNGVIGMTTLLLDTELT
                     AEQKGYLETLKHSGESLLRIINDILDYSKIEAGKFTIEHIPFDLRLTIEDTLDILAPT
                     AQGRQLELVGLIDAQTPRTVIGDPGRIRQILTNLVGNAIKFTEKGEVLIQVMQTDESS
                     GSVTLRFEVIDTGIGLTQEAQAKMFQAFSQADSSTARKYGGTGLGLSICKRLVELMDG
                     EIGLQSMPGLGTRISFTVKFSTPEEGTAPITPPLPVKNLSGLRVCLVDDNATNRSLLQ
                     YHVSAWNMHHESAVDGPSALNLLRQAAQEGTPFDIAIVDVHMPEMSGLELCRLIKEDP
                     AIAKTHVILLTASGQRGDSMLAKEAGAAAYLTKPIRERHLADCMRLTLGREGREEKAQ
                     LITRHTLTEVKAHTVQRVLVVDDNPINQRVAVKMLEKLGCRVDLAGTGMEALAAICRH
                     PYPLVFMDCQMPELDGFETTRLIRSQEQPGTRLPIIAMTANAMAGDREACLKAGMDDF
                     ISKPIIAADLQAKVAHWLPDVRPEDLKPSVANDAPAGG"
     gene            complement(69799..>72535)
                     /locus_tag="PK224_07380"
     CDS             complement(69799..>72535)
                     /locus_tag="PK224_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB15594.1"
                     /translation="KSEVLYVSPAFETIWELPRDLLYANPTIWLDHIHPEDRDRVAIA
                     VACQAHLPYDEEYRIVTPSEQVRWIRDRSFAIKNVQGETYRIAGIAVDITAAKQMEVQ
                     IRASELRHRALVELSPHAIFVNCEDKIVFANQACAKLLGAIAPSQLFGKTVLEIIHPE
                     SHPTVRERFAKIRDTNQPLPPTEERFVGLAGSIVDVEVAAAPIMFEGKPAIQVIATDI
                     QTRKDLERALLATNLQLQTILSSATNVSIVATNTEGTITTFNTGAEELLGYSAGEMIG
                     QQSLTLLHVPEEIDRHARELSDLYEHPIHGVAALIENAKRGGFDEREWTYQRKDGSRL
                     TVLLTITALRDSDGVVTGFLAIGKDITSRKAAEHALAQARDEALRAAQAKADFLATMS
                     HEIRTPMNAIIGMTGLLLDTALTKEQLEFADSVRRSSDSLLTLLNDILDFSKIEAGKL
                     HFEELPFDLRMTVEDTVELLAEQAQSKGLELIGLVDAAVPTAVVGDPGRLRQILVNLV
                     GNAIKFTSAGEVFLHVTRETQDGVGLLRFTIKDTGIGIPEAAQGRLFNAFVQADSSTT
                     RRFGGTGLGLAICQRLVSQMHGRIGVESHPGQGSTFWFTVQFPETTLAASPSPFSWSR
                     LHGRRILLVDHCDTVRQAMRQELTSHGMTCTVAQSGPEAVELARTAAAAQNPFDLALI
                     ELHLSDMDGFETAALLKQDPATAGVHLVILTTVGRRGDGQTARSIGIDAYLTKPLRQT
                     QLLECLCLLLSPETAAGTPSNAEPPPLITRHTLSESQAGPKPRLLLAEDNPVNQKVAC
                     KMLEKLGYRVDVAGNGQEAVAAHERARYPLIFMDCQMPEVDGFEATALIRKMEGRSAH
                     TPIVAMTANAMQGDRERCLAAGMDDYIAKPVRPKELQAVLDTWLGTAGAATGTTG"
BASE COUNT        14536 a        20026 c        22569 g        15404 t
ORIGIN      
        1 gcgcatcttg ggttgacgaa gtatttcggc attgcgcgtc ccaggatcgg cgtggcggcg
       61 ttgaatccgc acgcaggaga gcatggtctg ttcggcaacg aggagtcgac cagcatcgca
      121 ccggccgtca agctggccag ggcggcgggt atcaaggcca gtgatccgct tccggcggat
      181 accctgttcg ggaaggccgc tcgcggcgac tacgatggtg tggtggcgat gtatcatgat
      241 caggggctca ttcctctgaa gctggtcgcg ttcggaacct gcgtgaacct gaccgtcggg
      301 ctcccgatta tccggacctc agtggatcat ggaacggcct acgacatcgc cggcaaaggg
      361 gttgccgagc atgggagcct gctggaggcc gtgaaggtgg cggcccgcct ggccgaatcc
      421 tggtcaccgg ttaccaaaac gtcccgatag gaaagaaggg gaatacgtcc atggcagaga
      481 caggactttc cgcggcgacg atgatcgatc agcaggtgaa ggaattggat gcgatcgcga
      541 caaggaccat tcaggatttc aacaccacct tgggcactga acgcatgggg cagtggaaga
      601 tccgcacggt ggccctgctc cagcagcatg ccgggcagaa tgcggccgat gagttggcgc
      661 gaaaaaagcc cggtatggcg ttcaccaacg atttgatcga agagttcacc gacgaggtgg
      721 aaagttatcg ctcctacctc gtgacgctcg ccaaacgcat tcgggccgcg gcccctccgt
      781 ccgccggcta ggcggttgcg cgggattatg gcgtcctctc ttcccccagc cctgaaacgg
      841 ctcggtcaga attttctcat cgatccgaac attatccgca agatcgtgtc gctggcggcg
      901 cttggcccgg aggacactgt cttggagatc ggaccggggc ggggggccct gaccgccggt
      961 ttgtgcgccg aagcgggacg cgtcattgcc gttgagatcg atccacagtt gcagcctcat
     1021 ctgcaggaaa gtctggggca ttgccgaaac ctggaccttc ggatcgggga cgccctcgcc
     1081 tttcccttcg agaccttgcc gctgcgtacg gtggtggtgg cgaatcttcc ctattacgtc
     1141 tctacgccga ttctcttcgc gctgctcgat gcgcgtgccc gcctcgatcg tctggtactt
     1201 atgctgcaaa cagaagtggc gctcagactg gcggccaagc cgaacagcga ggactacggg
     1261 gtgctgtcgg tgttgaccca ggaggcggcc gaggtggaac tggcctttcg tgtatctgcg
     1321 aattgtttcc gtccacgtcc gactgtcgga tcggcggtgg tgcatcttcg gatcaacacg
     1381 caggaagggt tcgagccggt ccggcacgaa cggttccggc ggctggtgcg cgcgtcgttc
     1441 gcccatcgcc gcaagacgct ggtgaattcc ctgcgtgatg aaggctatcc ttccgagcag
     1501 attgcgcggg ccactgcgga agccggcgtg ccgccgcaag cccgggccga gatgctcacg
     1561 ctcgacgact accgcacact tgcgcgcgcg ttcgatcgcg atccggtctg actccggtcc
     1621 ctgtgagcgt gagtgggcgc ggcttcatcg gcttgcgcaa catttcccgt ttgtttgaaa
     1681 aggcgttttc cctgtgctac gatccgccgc atgggtgtat ccaccacggc aaagcgcggt
     1741 gacgcccgct cagccccttc ccgttcctct catgtgaagc gcgaagtgat cggggttctc
     1801 ctgatcgccg ccggtctcct gatcctgctc agtctggtgt cgttcgttcc cggcgacgcc
     1861 aaatcgattg cggcatccgg agcagccggc aatcagccca aaaacatgat cgggtcggtg
     1921 ggcgcgctgt cggcggccgc ttgttttttc atggtcggcg gcgccgccta cctctttccc
     1981 attcttctcg gactgctcgg cgcgcgttgc ttcacgccga tcccgctcac tatgcgaatc
     2041 agaaacgccg gcagcggtct ggcggccatg gtgttcttga gtgccctgct gcatttggaa
     2101 gtgacggcgg tgcccacgat ttccagcggc tgggtcaatc gaggtctggc cggcgggatt
     2161 atcgggcagg tgttggccga cggggtccga agttactttg ccacgaccgg cgcgcatatc
     2221 gtgatcctcg ccggcttgat ggtggcgctc ctgtttacgg tgccgctgtc gctgacggcg
     2281 ctgctgcagc gggttcccga ctggtgggcg gccgcgcgcg agcggatggc cgggctggtt
     2341 ccggagtggc cgacgaagca ggaggaggcg cagcccaagc gagtccgtga gaagaagtcg
     2401 cgagcttcgc gcgaggttga ggaacaggac ttcgaccgcg aagtgcaggt ggtggctgaa
     2461 gcggtggagc agattcccgt tcccgtcatt ccccctaaga ttcagccccc gatgaaggtc
     2521 gaaaaacgcg cggtgtcggc ggacgagccg gcagcagcag ttgcgaccag tccctccgta
     2581 tccgacggct atgtcctgcc tgatccccag gaactgctga gcgatccctc cggcccgctt
     2641 gcccgtctca gcgacgatga gttgaaactg caatccgaaa tcctgaccaa ggcgctgaag
     2701 agttttgcga tcgaagggcg ggtgacggag gtgcgaccgg gtccggtcgt gacgatgtac
     2761 gagttcgagc ccgcgccggg gacgaaggtc gcgcgcatcg tcaaccttgc cgatgacctg
     2821 gccctggcac tcaaagccat cagcttgcgt atcgtggcgc cgttgcccgg gaagtcggtc
     2881 gtcgggattg aagtgccgaa tcctcatcgc gaaatggtgt ccatgaagga agtcgtcacc
     2941 agcgacgcct tttcgcgctc acgctccaaa ttggggctcg cgctcgggaa agatatcttc
     3001 ggcggtgcgg tctgtgcgga cctgagaacg atgcctcacc tgctggttgc cggcgcgacc
     3061 ggagccggca agagcgtggg gttgaacacg atgttgttga gcattctgtt caacgcccgt
     3121 ccggacgaag taaaactgct gctcatcgat ccgaaaatgc tggagttcca aagttacgat
     3181 ggcattccgc atttgctccg tcccgtcatt accgatccca aatcggcggc gcgcgggttg
     3241 gggtgggtgg tccaggaaat ggagcggcgc tacaagctgc tggctgatgc gggagtgcgc
     3301 agtatcgatg cctacaaccg gcggatttcc gaggtgcaag gggccgtgtc ggatgtctgg
     3361 caatccggca agcctgagca ggtcgaactg acgtttctgt ccgaagagga gcgtctctcc
     3421 aagggagaag atgcggagcc cgccggcgac aatgggccga ctgattcggt caagccgagt
     3481 ccgccggagc ccctgcccta catcatggtc atgatcgacg agctggcgga cttgatgatg
     3541 gtggccccga aagatgtgga agataagatt gcgcgtctgg cccagatggc acgcgcttcc
     3601 gggattcacc tcgtgttggc gacccaaagg ccatcggtcg atgtgttgac cggcctcatc
     3661 aaggccaatt tcccggcgcg tatcgcgttt caagtctcgt ccaagaccga ctcgcgcacg
     3721 attctggatg ccaacggcgc cgaggcgctg cttggtcgcg gcgatatgtt gtatctcgcc
     3781 tcggggaccg ggaagctgat gcggattcac ggctcctacg tgtcggacga cgatgtgcgg
     3841 cgggtggtcg aatttgtgaa gaaacaagcg ttgccctcgt actgccgaga actccaatcg
     3901 ctcaagatcg aagaggcgga agaagagcaa gccaaggacg aagtctacga acaggccaag
     3961 gatctggtat tgtccaccgg ccaggcctct gcttccctga ttcaacggcg tctccgcgtc
     4021 gggtatcccc gggcggcccg tatgatcgaa cagatggaag cggaaggtgt ggtcggagca
     4081 gcaggccggg acgggcgtcg agaggtcctc ggccgtcgtg ggccggtcgg tggagatgag
     4141 gcatgatgcg gatattcata atcgttgctg tgctgctcat gaatggaatt ccggcagtgg
     4201 ctcaaccggt gaagcccgat gagccggcgg tggacctcca gtctctgcag gaggtccaag
     4261 aggtcgtgaa aaagatccag acccgctatg aaaagaccaa ggacttgcag gcctcgttca
     4321 cgcaaaaaac caggatcgaa ggattttcga cgcccgtgat ttcgaccgga catttctata
     4381 tcaagaagcc cgggcgtctt cgatgggatt atatcgaacc ggctacggaa gaaatttacg
     4441 tcaacaaaga cgacgtcaaa atgtatgtgc cggagcacaa gcaggtgctg gtgggcaagc
     4501 tcacctatat ggccgcttcc caggctccgc tgcaattgct gcaaggggtg gccaaattgg
     4561 atgaagagtt cgacatcgaa cccacggcga acaaggaccg tggcgcaggg ggcattctgc
     4621 tggtctcttt gactccgaag cagggacggg ccgaaccgga gcgcgcgatt cagaagattg
     4681 tgatcgaagt acagccgaag acgtacttcc tcaagacgat cgccctgcat gaggtcagtg
     4741 gcaatatcgc aacctttgag ttttcagagt tgaagccgaa cagcggactg aaggacgacc
     4801 tatttgattt caaggccccg gccgatgtag aaatcgtacg ggcgccggtg ctgagcagac
     4861 cctagctctc gacatccgtg acgcgtgaat cgtgaagcgt atctcgtgag agatcggagc
     4921 gtatagctca tggcttatag catatggctg aggatggtat gagaagctct gcgcctctga
     4981 tttcgggctc cctgtcgtcg gctattggcc attcgctctg cttcgcccac tgctgctgcc
     5041 agctatgcgc catacgctcg ttctccggac gagatacgaa cgacgcttca cgaaaggcga
     5101 aggtatgagc ccaacctgta caagcagcgt gaccgaggcg gtggagcggg cgattgcggc
     5161 gctggatctc gaccggcttg agcaggaata ttgggatcag aacgaattcc tgtatatccc
     5221 agagtttttg ccgcgcgatt ttgtggaggc caccctggct cagcaagccc agatgctcaa
     5281 gtcagggttg aaccgcaatt atattcccgg gcataagaag ggcggcagtg tcggctatta
     5341 tgccgtgatg gagaaagcgc cgcagttcat cgacctgtat cgctcggacg ccttccgggc
     5401 gatgctcaat cggttgacca aggtgaattt gctgctgtgt cccgacaacg atccccattc
     5461 ctgtgcgctc tattactaca cggaagccgg cgatcacatc gggtatcact acgatacgtc
     5521 ctactacaaa ggcgcccggt acaccattct gatgggctta ctcgatcagt ccaagcagtg
     5581 ccgcttagtc tgcgatcttt tcaaagatgt gccggggaaa caaccggtgc accgtgaatt
     5641 ggcgactgcg ccaggcgatc tggtgatctt caatggcgac aaactctggc atgccgtcac
     5701 gccgctcgga gagcaggaag agcggatcgt actgaccatg gagtatgtga cgaatcccga
     5761 catgggcgcg ttcaagcgcc tgtactccaa cctgaaagat tcattcgcct atttcggatt
     5821 gcgctcggta tttaagcggg cgtacgctcc tcgctcacaa tcctgacatc cttcgctgct
     5881 gcaaacctgc cgaccgactc ctcgcgtgtg tcttctcgca aggccgcaat ggaatcacga
     5941 catcatcgtg gcgcttcctc ttactgcgcg agggccggaa accgtaaccg gaaggtggtg
     6001 cctccgcctt cggtgctgtc caccgtgatg gttccggcat atttggtcac gatcttgtgc
     6061 acgatgctga gcccgagtcc cgtccctttg ccggctgttt tggtggtgaa gaacggatcg
     6121 aaaatcctcg ggcggatgga atccgggatg cccggaccgg tatcggcaat ggtcacctgg
     6181 atccatctgc cgtccagacc ggtcgctaac gtcaagcagc cgatcccctc catggcctgg
     6241 atagcgttgc tgatcaagtt gacaaacacc tgatcgactt ctccctttcg cgcgcgaaga
     6301 aacacagggg cttcgaactg tgtggcgatc tccacgtggc cgaaatgagg ccctcgccgg
     6361 accatcttga ccgcttcgag caaacgttct gccacatcga tttccgtctc tccatccctg
     6421 ccggccgcgc gggcatagcc ggagaagtcc cgcacaacgg tggagacgtg acgggcgtag
     6481 cccacaatat cggcggcaaa ctgttttatc gtgtccggat cgtcctcatc ctggatcaac
     6541 tcggccatgc tgaggatagc ctgggccggg ttgttgattt cgtgcgccat accggacacc
     6601 atggtgccaa ggctggagag tttttcagcc tggatcagtt catcctggag ttgtttgtcg
     6661 tcggtgatat cccacaacag gatgcccaat tggtactgcg gggattccgg ggtcgagacc
     6721 ggaaaaaacc gatagcgata caaccgtgcg gcgacttcgc actcacgttc agagagccga
     6781 tgaggcgagt ctgacgtgtg cgtgctccat tccagcatca gctgttggag cgcgggctcg
     6841 ctgagggaaa gacattcgca cagcggacgg ccgatcagcg attcgccggg aggcgcaaaa
     6901 tactgatggg ccagcgcatt ggcatactga acgtgccagt cgcttccgca caggaaaatg
     6961 gcgccgggca gactggccag caggctctga gtcatgtgat gcgccgcacg taatgcctgc
     7021 gcggctcctg tgcgatcgat gaactgtccg atcttcatgc ccgtgtcggc gaggatctga
     7081 atcacgtggc tgtcggaggc ccgccgctgt tcggtataca actcaagcac tccgtggatg
     7141 atgctgccgg tccggatggg gacgatgcag gccccgtgaa tacccaggcg agaggcggtc
     7201 ggtgcgcggg tgaaccggga atccgccagg acgtccgata tccagagcgg gagttttgag
     7261 gcccaggcga tgccagggaa atccatcccc ggcgggaact cactgttgcg acagaccgag
     7321 agaaattcat cccatcgatg tgagggacgg atccagcctc cctgacaagt cagcttgtct
     7381 cccattggcc cacattgcca caggagcgcc atatcccacg cacccattgt gcctgcgacg
     7441 gggagaagtt gcgggatggc ttcttcgacg gtgccggctt gcgcgaggat atggctgacg
     7501 gagagttgtg cctgctgtag gccctcctgc tgtttgcgag cgctgatgtc ctggaagacc
     7561 acgactgcgc tgaccggccg gtctcgctca cggatgggac tggtgacgta ttggacggga
     7621 aacagcgagc cgtctttcct ggcgaaggtc tcgttctgga tgcggtgaat ggcgccgtca
     7681 tgaagcgctg cgcggagaca gaaagggtct gaggcgtaca gcgcattgtc gttgccgatt
     7741 cgatagaaga gcggtgccaa cggcttgccg atcagttctg ccgcgctcca tccgagcatc
     7801 cgcgccgcag ttgcgttgac gaaggtggcg catccttgcg ggtctagtcc gaagatgcct
     7861 tccccggcgc tgtcgagaat cagcgcgttc tgatggctca acctgacgac agactcttcg
     7921 gcgcgtttgc gccgcgcaat attgcgaatg atgccgctaa agaacatccc ttcactcgca
     7981 cgccaggatg ccagcgagag ctcaatcggg aattcgtctc cggtttttgt caggccgacc
     8041 atttcgatgg tatgcccgat gatcgtggct gttccaccct gggcgagacg tgtgacgccg
     8101 gccgcgtgcg cggcgcgaaa ccgttccggc atcatgatgg tcagcgggcg cccaagggcc
     8161 tcttctttgg tgtgaccgaa cattcgctcg gcggcgccgt tccaccagat gatgaggccg
     8221 ctctggtcgg cgagcaccat ggcgtcggtc gccgactcca ccaacgcccg gaagcgttct
     8281 tcgctggcct gcagcgcctc ctctacatgc tcggcccgga cggccagccc tttcattgaa
     8341 acggctcggc gaaggatggc tttgagttct tgggaattat agggcttggt cagatacgcg
     8401 aaggctcctt tggccagaga gccgatcgtg ttttcaggcg tgacgtttcc ggtcaggatg
     8461 atgacgggga ggctcgcatc taattccatg agcactttca agaccgagag tccgttgagg
     8521 tcgggtaatt ggatatcgag gagcgctgcg tcgtagcgcg tctgtttggc ttgctgaagg
     8581 gcgtcggttc cggaagcggc gctgtgtacc aggtatccct cgtgacctaa catgtccgtg
     8641 agagattccc ggatatccgg atcgtcatcg actatgagaa tgggagggcg catggtgcga
     8701 ctgttccgga ttgtgagaag tcttcacgat ttgctgtgag cgcaatacct atgccgccgg
     8761 tgcgccgatg aagcatgggc caacagagca ggaacccatt gattctgtga ggaacagcga
     8821 gagagggaaa ccgtgtgcgc cgtgtcgcgc tgcctgcgag tgttcaaata aatgcggggt
     8881 ttatcggatc ggatacagcc gaggggacgc tgtcgcacta ctacatagcc tgctacggcg
     8941 tcgatgcggg tgagttgtcg agaggcaagg taaagtagaa ttgagttccg acaccgagcc
     9001 ggctttccac acacatctgt ccgccgtgca gggccaccag actcttcgtg atgaagagcc
     9061 cgagttgcgc cccttgtgaa gagggcatgg ctgattcgac cttggagaac tcattaaaga
     9121 catggggtaa ctcgtcgggg gcaattccgc agccggtgtc ggccaccgta atctgggcgt
     9181 acctcgcgtc gcagatgctg cagctcactg agacatgtcc tgaaggtgga gtgaatttga
     9241 tggcgttgcc gaccagattc caaagaattt gctccagctt gtcgcggtcg gcacgcacgg
     9301 tgatcggttg ttcgcaggac aggacttcga ggacgatgga tttttcggcg gcgagtgtct
     9361 gaaagctctc caccacatcc cggacgaact catcgatgtg cagtgattcg ggcttgatct
     9421 cgatccggcc gacatccagg cgagaccagt ccagcagttg attgatgatt cgtgtgagtc
     9481 gatccacatt gtgtttcacc cgctggagat agtgctcttg ccgctctgag agttgaccgg
     9541 tgaggccgtc cagcatgttc tcgacgaaac ctttgatgga ggtcatcggc gtgcgtaatt
     9601 catgcgacgc gaccgacaca aatttcgagc gccgttggtc gtgctcctgc aggcgctcgt
     9661 tcaccgcttg caattcatgc gttcgttcct gaacgcgtcg ttcgagatgg tccgtcaacg
     9721 tggccagttc ggtataggcg cgcgcattgt ccacggcaac cgccacgtga ctggcaatgg
     9781 tgaccagaag atcgagatct tcctggctgc agggatgggt gcctcggtcg gcgcccaggt
     9841 agccgagaat ccgctgttga ctccgaagag gcgcggcgac gaaggaggag attcccacct
     9901 gacgaagcca gggaagcact gcgggggaga tgcgatcggc gaccggctca atgctcggaa
     9961 cgaacagggg cttgccacgt atcagaagtt cggcggcaaa cccgccgtct tcgcacacgg
    10021 gaatctcaag ccgccgagcg gcttcttcca cttccggggg catgcccagc atttgcccca
    10081 ccgtcgcgat gcctcgctcg ctatcccata aaaacaggac catccgggcg aatcccaaat
    10141 tttcactgag cagctgcaat acaattgaga ggagccggtc aaggtcgagc gtggaggtga
    10201 tggctgtgct ggactgattc aaggtggtca gttgcgtgac atgtcgccgg atggtttcaa
    10261 ggttggcgga gatggcctga ttgcgttctt gaagagattt ggtcatcaaa ttgaacagcg
    10321 ccgtcagttg gccgacttcg tcctgggtcg tcggaacgac aaaggtggaa agatcgccct
    10381 cgctgacttt gcgtgcgcct gcggcgagat ggcgcaaggg agtgatgatc cggcgggcca
    10441 gcagattcgt gcagagaatg ccggccgtga tgatgccgag ggtgagcagg agaaagtttc
    10501 gcagaacttt cgccagttct cgttgcatcg gcacctcggt caagccgatt tggacgacgc
    10561 cgaagacctg aggggtggtc ggtgcgttcg ccgctttgcg tgtttcttcc tcctggagcg
    10621 ccagcgcttc caggcgggat tccgagcgct tcatcaccgg gatggcaaaa tcgtagaacg
    10681 tctcttcgtg aatgcccgat gtcgaaattg gaagggtcga gtccttctcc cgcagaggga
    10741 ttgtgtccgc accgctgagc ctgaggcgcg tgatggtcgg ctccgtgcct gaatgcttga
    10801 tcaccgcgtc cgcaatgtca ggacgcggat aaaaaggtcg ttctgccgat cggacggtcc
    10861 cgagcgggct gtcgagtttg cctttgctct ttgcggcgag gatctggccg tctgcgccgg
    10921 tgatcagcgc atagaccacc tcgtcgactc ccatgacacc gtcgatgaac tgctccagga
    10981 tgactcgttc ctggatgatg atgccgtatc gtacgttgtg ggcgagattc ttgaccagaa
    11041 tggttccgag atcttgcact cgttcggtca tggcgatgcg tttactgtga atgaagtacc
    11101 aactcattcc cgagcaggtc agaataatga tcaagctgaa aaatacgacg aacttcgtcc
    11161 tgagactgat aaatcgtccc gggccgctcg ggtacaacga gggtgaggtg aggtagtgcg
    11221 cggggccttt cacggtgggt cctttcatca atagagctcg tcggcttttt cctcgaggtc
    11281 tctggggatc tcgatgtcga ggaacttggc ggttttacga ttcagactca tttcgatacg
    11341 gtccgggtgt aaaggttttg cggggagtga gacgtgaccg tccaggatct tgcgggtgag
    11401 ttgccccgct tgtcgtccga tatcgccata gttgaccgac aggcacagca gggccccgct
    11461 tcgggcgaac tcgcgggaaa atccgaccac cggcacgcgt tcctccaatg cggcattcaa
    11521 gataaaccgc agcgattcat cggtcagcac ggtggagtca gggacgagcc acaatgcgtc
    11581 gatcgacggc agcaaggtgc gcagagcgcc cggcacatcc cgctcgttgt tgagcgggat
    11641 cgggacaagt tccgtcccgt tggatttgag cagtcgccgc gcgtcttcga tcagcgtcgc
    11701 ggttttggat ggatcgtaca aggtcccgac gtgtttgaga cgaggcagta gggatttaat
    11761 catgatgagc tgccgctcca ttggaatttc catgagcacg cccgtcatat tcggggcgtt
    11821 aagcccgtac ttggccggat ccagcaccat ggcatagacc actggaatgt cgacgatctc
    11881 gagttgtgcc gccttcgccg ccttcaagcc gacggcgaac accagtgccg ggtcggctgc
    11941 acggatcttg cgggtcagtt tccgtccttt ctccaaatca ccttgtaaat catattcggc
    12001 gagcacgacg tcgtttggaa gggcggcttt gaagccgctg atggcttggt tataggcggc
    12061 gatgtccgac gacttgagaa tgacgacgtc cgtggccgag gccagcccgg gactgaagag
    12121 ccgacagacg agggcgatga cggtccaact caggctccgt cgaaaccgat cggcggagcc
    12181 cgtggtggag ggtgattgta cgaatgggaa cgtcacagtc tgtatcctcg tcgatccagc
    12241 cggccccgtg agcgattgcc gaatgccccc tgtctacgtg tccgagtgcc cctttatcgc
    12301 gtgaacgata ccgaggacgc gtgtggcgac cccgtcggcg tggcgatgaa tacggccggt
    12361 ccaggccagc caatggatgc tgtgatcggt ccacacgacg cgatgccgga tgttgagagt
    12421 ggcctggtcg tgcaaactgt gttcgatctg catcaggacc gacccgcgat ccagcgcata
    12481 aatcaggccg atataagcgg cataggtgcc gggaaaagac ccgggggtca gaccgaacaa
    12541 gacctcgacg tcgggtgacc agtagagacg gtcggactgc agatcccaat cccagatgcc
    12601 gacttccgat tcggtcaggg ccctgcgcaa ccgttcttcg ctgaggcgta ggtgcgcttc
    12661 tgcctgcttg cgctccgtga tgcattgcag cgagccggcc atgcggagcg catacccctg
    12721 cgcatcccga tccaacactt cggcccgcgc gtgaaaccag cagtagtcac cctgcttggt
    12781 cagcaaccgg tagtcgacgt cgtaatgaga gctgcctttt tcgagacagt ccgtgagccg
    12841 atcgaatacg agttcgcgat catccgggtg cagtcgggag gcccaactgt cgaggatatc
    12901 gggaaattcc tgctcgttgt atcccagcat ctcgcgcacg cgcggcgacc accagaccgg
    12961 agtcatcggc gagtgccagg gctggtcggg aagcacatgg ccgtcccaga atccatcgtt
    13021 cgatccgcgg atgaccgatt cgagtcgctc ttcgctggcg cgcagcgatt cctgaacctg
    13081 tctgtagtcg gtgatatcac gggaaatagc caggaggcgc cgggtatagc cccgcgtgtc
    13141 gagcatgggg gtgacctgta catcccacca ctttgcgatt ccgtcggtgc tagggcagaa
    13201 gccgacgaac tttccgacct cgccggcctt ggccgcttct aatgcagcca gggcggcatt
    13261 ccgatcatcc ccctcccaaa gatccaccca aggtctattg aggtaggcat tgagatccct
    13321 gatatgtaaa atcgtctgcc cggcgtcgtt catgtattgg agccggcctt ccaggtccaa
    13381 tactttgatg cagtcggcgc tgctacgtaa gatttgattc ttaaattctt cgctgtcgtg
    13441 gagggcgagt tccgcctctt tcatggcggt gatgtcctcg cacacgatca gcacaaccgg
    13501 agcctgttgg tcgttcagca cagcctgggc ggtttctcgc acccagatga cgctgccatc
    13561 cttccgaacc ttgcgaaact cccaacctct gggccggccc atttcggaga ggcaggcggc
    13621 aaggttccgg cgtacgtcgt cctgatcgtc ggggtggaaa atgttcaaca cggacgatcc
    13681 gagcagttcg gggacgtcat atccgagacg atcggcgccg aagcggttga ccgacaggac
    13741 gatacctgcg gcgtccacca tgaaatacat ggacggattg tcgtcgaaca gggcccgata
    13801 ccggcgttcg ctttcgatca gcgccgcttc cacccgtttg cgctccgtca cgtcctccgc
    13861 gaagcccgcg atgcgataga ccgtccccga cgcgtcacga atcgggaatg cgcgatccca
    13921 gatccatcgc accgatccat cggggcggag gatgcgatat tcctcactgt acgctcccga
    13981 aatttgccgg ctcatggccg cgtcccggac ccgtagccgg tcgtctgaat ggatggcgtc
    14041 catccacgag cccggcatcg cgtagaggct ttcgcaggaa cgcccccaga cttcttcgta
    14101 cccgggactg atatagagca ctctggtctt ggccggatcg gtgatccaga acacttcccg
    14161 gatgtgctcc gccagctgga ggaatcgctc ctggctttct tcgacggcga gttcggcttc
    14221 caggcgctca gtgatgtcac ggaagacgac cacggcccct gccaccgtgc ccttctcaat
    14281 gatcggggtg ctcacatatt ccaccggtat actggtgccg tcctttctcc agaagacatc
    14341 gcgcggcaca cggtggacta cgccgtcgtg aagggccgca tagatcgggc agtgatcggc
    14401 cggataggcg ctgccgtccg gccgggaatg gtggagccgt gtgtgcatcg attggtcgat
    14461 gagttcggat gggtcatatc cgagcaattg agcggcggtc tgattcacga aggtggtcag
    14521 gccgtcgcga tccagtccgt agatgccttc ccctgcttgc gtgaggatga cttcgtgcag
    14581 atggcgaagg cggtcgagaa tctcttctgc cttgcgccgt tgagtaatgt cgcgaatgat
    14641 gccgctgaaa aagacggcga catcggtttt ccacatggcc aacgacagct cgatcgggaa
    14701 ctcgctgccg tccttgcgca ggccctccag ctcgaccaga cgaccgataa ggcgggactg
    14761 accggtgtcc ttcacgcggg cgagccctcg ctcgtgagcg acgcgatacc gttgcggcat
    14821 caacatggtc aggggttgcc cgcggacttc gtcatcggta tacccgaaca agcgggagga
    14881 ggcttgattc caggagagaa tatgaccgtt gtggtccgcc agcacgatgg agtcggtggc
    14941 ggcctcgacc agagatcgaa atcgtgcttc gctttcgctc agcgcgtgtt cggcgtgctc
    15001 ggctttggcg gcgagcgcct ggatgccgat cgcccggcgc aggacggccc gcaattcgtc
    15061 ccggttatag ggtttggtga ggtaggcgaa tgcgccctgt gtgagtgaac cgacggtacg
    15121 gtcggcgctt gtgtaggccg tgaggatgat gaccggaagc tgcggttgtt gttcttgcaa
    15181 tgttcgcagg accgaggagc cgtctccgtc cggcaagccc aaatccagca agacggcatt
    15241 gtactgatgc tcatgcgctt gcgtgagcgc ttctgcgcag gtgccggcga cgttcacctg
    15301 atagccgtcg tggtcgagta agtcttgtag cccgagggcg atgtctggat cgtcgtccac
    15361 gacgaggatc gaatggccgg gtgtaatggc tgtcatagcg gcctcgctgt cactccggag
    15421 ttctcatggc ctgaaacctt gctctgagca tgccgatgat tccgtcaaaa tctcacagtg
    15481 acccatccca tcgaccgact gccgatccgg tcgccgagcg gatgctcccg atgcttgtcg
    15541 ttcagtgcat tgaagatcga gaaggcgact tccgcgtcgc gcatgtaccc ggcggcggcc
    15601 ttttgttgcc agaatcgata tccgacgcgg agattgagca ggttgtaact gtcgacgcga
    15661 tcccccggcg cgatgacgcc ggtcgtcgga atactcgcca atgtcgtgaa ggtttgtgca
    15721 atgggatacg tggttgcgcc gacatagtag taactgattt ccccgctcag gccgttgtcc
    15781 cattcggtcc tcagacccgc gctgactttc gatcgcgggg caccccgttt gaccgtcccg
    15841 ctgaacgact ggccgatttc ctcataggca tagttggcaa atccgctgag ccagcggctg
    15901 gcgagaaatt caattccggc ctcgccgcca tagatgtccg cagagccctg gtcgttgacg
    15961 aattcggaag cgccgccgga ggtgattcgg ctgccgatca gatcggagac atggttgaaa
    16021 aataggtccg cccgcaccct caagcggtgt ttccaatacc agccctgata gccggcctca
    16081 tacgaaatga tctgttcagg ggcgagccgg gtcgagccgc tgatcggaac cgtcgacgag
    16141 attgagggcg gaaacggcac accagtcgga atggtcgtgg tcacgcgttg gtcctgatgg
    16201 gattcgaaga gggtcggggg ccggtaggca gccgatccgg agaggtgaaa ggtgtggccc
    16261 tcaacgggct ggtagaggat ggccacacgc gggctccaag tcccggtgat ttgtgtgtgg
    16321 agatcgtagc gcagcccggc gacgatcgtc agcgaagatg cggcgcgcca ttcatcctgg
    16381 atgaagagcc ccaggcggtc ttcacgactg aaccggtcga tcgtcgtgct cgacaatgag
    16441 ttgtggcgat acgtggcgcc atagagcagc cggtgagtcg cccacagatt ggttgcatgt
    16501 tgcacatcga cgttataggt attcccggag aagggattgg tcgattgtcc gttgcggtcg
    16561 gtaatgctca ggagatcgac caattgagag ttgggagtaa tcgttgcgtt gtccgtgtat
    16621 cctgaccacc aggctcgaat gagaaaggcg ccctgctcat agaggacatt ggcgtaagcg
    16681 agcgatggcc ggatcgaatt gctggtgact tccccgacct gcccgtcgta ccgattggtg
    16741 tcgaccagcc cgcctgatac ctgcagtttg gaagtcgacg acaaggcgta ctcggtctgc
    16801 acattgaact tattcgatcg aaaccccaga gcatcacgat cccgccactg ttgtgtctga
    16861 tcgcgaccga tggagaggcg gtaaccgaac ttcccgatgg tcccggcatg gaccgcggcg
    16921 ctgctgatcg tgccgagttc acctcctccg aattgcaggg tcgtgccttt catctcttca
    16981 ggcgatttgg tgatgatgtt gatgaccccg tcgaaagcat tgaacccata catggccgag
    17041 gcgggaccct tgaggacttc gatgcgttta atttcaggta gggtgaccgg aatggatttc
    17101 cagaagacgg tcccctggac atcgccgtag atggatcgtc catccaccat gacgagcatc
    17161 ttgtttgcga agggctggtt tcctcccctg gcactcacat tgaagtcgcc gcctgtcatc
    17221 tgcatgattt ccaggcccgg aacgcggcga aggacagtcg gcaaatcaat agcgccggat
    17281 tgccggatat cttcgtccgt gatcacatac acgttcgacg gagcctggga aatcggctgt
    17341 tcataacgag atgcaatgct cacggtttct tcttctttca agagttccag gtcattgctg
    17401 acggcgggag tcatggactc gtcgatgacc ggcggcggtt cggcagccgc aacgctgctg
    17461 aggcacagga gcaacagcac tgccgacaac gaataccagg ctgtgttcaa ttcaaaagac
    17521 gtcacgttca atttaaaacc tcaaggtgac ccaacccatc acgcggcttc cgagcgtatc
    17581 gcccaaggga tgttccttgt gtttgtcgtt gagcgcattg tagacggcca cggccacttc
    17641 agccttgtca tgccagaatc ggtatccgcc acgcaggttc aacagggtgt aactatcgac
    17701 cgtcggactg gtggggctag gcagcaccgg tgcgagcgcg gtaaaaaatt cgatcaaggg
    17761 gtaagtggcg cttgcaacgt aatggactgc gatttctccg ttgaggccgt tgtcccagtc
    17821 gccgcggagg cccacgttcg ctttccagtc cgggccgccg cgccgtgaga cgccggtgaa
    17881 ggtttgtccg atcttctggt aggacacatt cgcaaagccg ctgagccagc gagtggcgag
    17941 gaactccaag cctgcttcgg ttccgtagat atccgcctcg ccgccgttga cgagcgtgcg
    18001 cgcggtgccg acggtttgga agttgatcaa gtcggagagg tgattaaaga agaggtcgag
    18061 cctggtccgg agccggtgtt tcatccacca gccctggtac ccggcttcat aggaaatgat
    18121 ttgttcggga ttgagtttct gtgacccaag cagcggaatg ggcgcgaaca gcggtggaac
    18181 ggtcggcagg atgcgaaggt caagattcga ttcgaatagg gtcggggggc ggtaggcgac
    18241 agatccgctg atgcgaaacg tatgctccgg cgtcaggttg tagacgaggg cgacacgcgg
    18301 actccaggtg ccgttgattc ttgtgtgcag atcgtagcgg actccggcca cgagggtgag
    18361 agatggacgt atcttccact cgtcctgaag atggaccccg agacgatctt ctcgccctaa
    18421 ttctgagacg gcactgccgt cgactgtgtt gtaacgataa ttgacgccat aactcagccg
    18481 gttggccgag ccgaattcga ggctgtgttg tgcctctatg ttataggtgt tcgtggtgaa
    18541 agacacattg gacccgcccg tcgcgttcgt ctgccggaca aattgcgaga ggagcgggtg
    18601 cgtggcggag tcgacgggga tgtcgttgcc ctgccaccag ccgcgcacgg agaaattgcg
    18661 gtactggtaa tgcacgtccg tatacccttg attgaattgg gaacgaggaa tctggacgtt
    18721 tgaaacgggg ccgtcgaagc gattggtatc gatgaaccct ccggcaacgc gcaaggtggc
    18781 gtcaccggag aagttgtact gcgtctgcac gttgaacttg ttcgaccgga aggcgagtgc
    18841 gtcattgttg cgccattgtt gattctggtc atggccgatg gagagccggt agtcaagttt
    18901 gcccgctgat ccagcctgga cagcggaact ggagatggtg ccgagttcac cgccgccgaa
    18961 ctgcaacgtc gtgcccttca tgtcctgagc ggacttcgtg atgatgttga tcaccccgtc
    19021 gaaggcattg aagccgtaaa caacagaggc gggtcctttc agtacctcga tgcgtttaat
    19081 ctccggaagg gtgaccggaa gagatttcca gaacacgagg ccctggccgt cgatgtagat
    19141 cgagcgaccg tccaccatca cgagaatttt gttggcgaag agttgattgt cgcctcgtgc
    19201 gctgacattg aagtccgccc cggtgacctg catgacatcc agcccgggta cgcggcggag
    19261 aatagtggga atgtcggtcg cgcccgagtg ccgaatgtcc tcgtcggtga tgacgtagac
    19321 gttggagggt gcttgcgaga tgggttgctc atggcgagtg gcgatgctga cggtttcttc
    19381 ctctttgagc agttcgagct cttcgctgac cggtgccgct gctgttggag tcgtcgcggg
    19441 aggcgatgcg tcctgagcat gagcgccgat ggcacagcag cagatcaaga gtccgccaca
    19501 tagtggaggg gcagagtgtc gtaaccgatt tctggtcggt cctgtcgcca cagggtgtcg
    19561 cttagccggc ggagaaggag atagggggga ggagacggtg ccgggggggt gcatcagagg
    19621 gcgcatggca taccgtggat cccggcccgg cggtgtcggg catggttgga cagaagacaa
    19681 gcacgaggac catatgatta gaagtactct cacgcaaaat gcatgcttat gggattctcg
    19741 gtatgcggaa agcagacgcg tgaattatcg ggggattcac gcgatttcgg ccgtctacgc
    19801 aaggagcgag gttgagagag gagctctcgg atcgaagcac gtatctagtt ggatacaggc
    19861 tgtatgtctt cagatacagg cgcccgattg gttttatttg cgcgtggacg acctggcttc
    19921 aaatcttgaa gccgtggcga aaaagggcgg ccgttttgcg actccagatt attgaggaga
    19981 tcttcaagcg ctgatgaaat caggccggcc atagtggtac ccgacgtcca ataggcggca
    20041 tcgcgcatac gttccaagag ctcggtcggc aggctgacgg tcatgcgttg gcgtttctgg
    20101 gatcgcagat agataaatcg attcatggtg ccactccttt gcacgcgaac actgatgttg
    20161 agcgcttggc ccggcgcctg cgtcatatcg atgcgcgagt ccgttgcgca gggatgtggt
    20221 cagtgtcatg aagttcaccg ctgcggcgga tgcggttcag gctgctgcgg ggcggtgtct
    20281 ttccatacgg caacaatcaa gagagctagc atgccactga ctccgatcag gagaccaccc
    20341 atgacgagtt ccatgtcagg ctcctttctg tactgaatgg gtcttcccgg ttacctcagg
    20401 gatggaaaag tccctgtaca ctcctgggaa tagcaacagc cgtgccgtac gtgcctgaga
    20461 aaaaagtgtt cgccgattct cccaaacctt ttgagaaatt ctgcattggg agagcgagct
    20521 gacaagaacc gttgtcttta cgcgggggcg cctcgcgaac agcgcctgta tcggatctca
    20581 ttcaccgcgt atcagattgg ataacggggc tgaagcaatg caggaagagg atcttatgta
    20641 ggcaggttca ctccggactg tttcaagagg tgatgaagca ttcgctccaa cgactgtttg
    20701 tccccctcat atgttatgag gtcgagcaga caggcgatgg cgtcgtacca gaggccggat
    20761 tcggaataac gatagatcgc gtcacgatcg caagaggtcc aggtgcatgg catgcccagc
    20821 gagcaggctt cactataggg gatccgttcg atggttccgc ctgcgacaat gtcccgcgaa
    20881 ggttcatcag ggtccacgac caaagtgatg aaccatcggt aggattcctg ctccttgagt
    20941 tcaattccat attcccgagg cctggtacag tggatgcctg catgaagagg tgaggggagc
    21001 gcgcgttcaa gaataggccg aattccgcgg gagtccacca ctgtaacggt catggcgtga
    21061 gtggtaggca gagacaggta ccaacagagt gacggatcgc ctttgatcgt aaaggcgaca
    21121 tgatccggga ccaaggcgat cagcagggga gctcccgact cggcacccct gctctgcccg
    21181 ccgatgcgcg cccgcggcgc atggttcttg cgcggtttat acaggggcat aggcgagtca
    21241 ggagccgtga cgaggtcggc cgcgcgaccg tccggaagtg ttgacgaaag cccgacggcg
    21301 ctgtgccagg acaccggcca cgccatagcc acgacaacca acagggtgag tctgaggtag
    21361 agcattctgt gccgattagt agttgaacgg actcgcgcag cttgtcagcg ctggccggca
    21421 tgggtttgcg caagatccca ttcggccacg ccatgcaagc cgacttgctt gagtaaggcc
    21481 gcccgctgtt ttcgaagacc cgtatctccc ggatcggagg taatgagatc gcacaagcag
    21541 gccatggcat cgtaccagaa acccgttgcg gcactcgtcc tcaccgcctc ctgagtgcag
    21601 gtggtcgtgg cgcccaacac catacactcg ctgaactcac agcgctcgat cattccgccg
    21661 gtcacgatat cgcgtgaggg agagttctcg tctttgatca cggagatata ccaccggtat
    21721 tggatgtgtg cctgaagcgt gaagccgagg tcctcaaggt tgacggcgtg aacaccggct
    21781 tggttcgggg gcgtgatgag ccgttcaacg agcgcgcgaa tcgatcgctc atcgatcagg
    21841 gtgaatttaa tcggcaacgc cgtgtgcttg gataggaacc agttgagaac cggatgttgc
    21901 ttgacggtga tcccgacgtg gtcgggaacg agcgcggcaa tagcggggtc ttctccttcg
    21961 ctgcctctca aggtcccacc gattcgagct ctgggcatca cttgctgagg cgccttgtac
    22021 agcggaattt ccggttgtac gtcagttccc gcctcgctgc tgctcgcgag cagggccgcg
    22081 agcagcaccg ttgtgccgac cttcacggcc atgcacaagc aacgccgcaa cgagcgaggg
    22141 ggcgaggcgt acccttcagg gcacggtgag ggcccgagcg atgcgagaac gctgatggcg
    22201 aacgttttta acatcctgct agagccaatt gttcaataac agaaatgccg accaatacgc
    22261 cggatgttca tagagccggc cggacagtaa cttcagctgt gcaatttgca aagcgtgtgc
    22321 cttagatatc gatgagtcat gcaattggcg ataaaattct gataccagtt ctgacgacgc
    22381 ttcatcgtta atgaaccaga gggttgcgag ggcactcctc gctccggctt tcaccgcaat
    22441 gccggcaagc ccgagcgcgg cacggtcgtc tccgataccg gtctggcagg cgctcaacgt
    22501 gaggagttcc aagggatcct ggcgaaagcg aagcagccct accagccgct ctaactgcgt
    22561 catggtcagc cgatcgtcga acgtcagcaa gaatgattga ttcacgtccg tggcaaactg
    22621 gccgtgcgtg gcaatgtgga gaatgctgta gcggccgtct ttcaattctc cttccaagcg
    22681 tggcgtgaca aagttgctgt cgaggagttg ctcgcctttg aagagcgagt tgaggtgggc
    22741 catctcttcg gcgacgtgcg ggagaggagg aaaaccctgc accgccttgg tcaaaccgct
    22801 ggagagtagt tgcgctctgg ttcgattgat ggggcgtggg tcagtcaaat cgaggccggg
    22861 ggtcattgcg acggcgtatt gtgcgatgag gaattggctt ccgtcatgga gcgccgacat
    22921 gggaatggtg cgaagcggtc cgtccgggac aaagaccagc gtgttgacct tgagtctgcg
    22981 aaggtccgct agaagcggtc gaagcaacca gtcatacaat tgctgcgcat ggggcaggta
    23041 ctcgcgggtc gtccgctttt caagcatgcg ccggaagctg cgtatctcgg cagtcagtgc
    23101 cgacgccgtc accggaacgg agtgtcgtct gaggccgtcc gggaagctca cgagtagttc
    23161 ggtgcgatcg ctgaacatga tcgggtaaat gatggccgtc gcaggggata cggcatccag
    23221 cttggtcagt cgggcctgta tctggtccac gcaatcatcg cgaaaataat cccggagctc
    23281 ggcggctttg taggcctcga ttgcgtcgcg cgccgccttt agataggagt cggcctcaga
    23341 gtcctctgtc atcaaagccg cccgttgcag aagcagatcg gccaattcga aatataacga
    23401 acgaatcgat tgatgggcag gatccagcga ggcttgcgaa ccggaggcca cttcggcgcg
    23461 gatcggttgc agggtggccg ccgcttcttg ataggccgac agtgcgccat cgaggctgcc
    23521 cagacgagtg aggagacgac cactttgcca ttgccaccgg tagagtgatt ccggtgcgcc
    23581 ggcagactgc gcagcaaaga cggctcgccg ggtgagttgg agggcttcgt ctaagcggcg
    23641 ttcctcctca tagagatgac cgaggtgccc gtaggcatag gaggccaggc gctgaccgcc
    23701 ggaatcctcc gccatcgcag ccgcttcttg cagtagcgca gcggaccgct tgagccagcg
    23761 cgcatgctca tccggctgaa cggtcgccag gtcgcgatac cctaacgcca gattgataag
    23821 cccatcggcc ttgttgtggg aaccggcagt cgctgccagc cggtcgtcgg cctcattcaa
    23881 ggatgctgcc gcctcatcaa atcgtttcaa ctgcatcgcc acccgcgcgg cgttgacgcg
    23941 ggtgcggacg aacagtatgc gtacattctc ctctgtcgct cctgccgcgc tgtcttgaaa
    24001 tgcggcaagg gcttccggaa aacggccttg taacgccagg acaatgccga ggtcgttccg
    24061 gagcgccgcc gaaagtgtct tgagattccg tgtgtgcgcg atctctgcgg cttgcgacag
    24121 atagtccgta gcggtgtcgt attgatggct cgtcacatac gttttcccca actcagagag
    24181 ggtgagcgcc agccattgtg gatcgccgat tcgacgcgcg agttgcaagg ccaattcctg
    24241 ttgcaggaag gcttggttca catgccccaa tgctcgtgct gcgtaggcgg catggaagag
    24301 cgcctgaaga tgtcccttca cctggcccgc ttggtcgtat agttgcgcgg ctgccttcca
    24361 atcgacgtgt gcctgctcca aagcgccccg ttccgcggcg gaaaggcctc gctgcatcag
    24421 ttcatcggcc gacggcccgg tttccgcagc ggatgccgct gtgccggaga gcaggaggat
    24481 gagcccgact ctgagcaccg gctgaagtga agcgcgaagc cgtgatgcca tgtcatgccc
    24541 tttctgccgc agtcgggtcg gttacagcgc ctgcacgacg agttggagat ggataccgtg
    24601 atcctgcatg ttcccctcgc cgccccggac atgattgagt tgctggcccc agtagacttc
    24661 gaagcgtgca cgttcctggg gcagaatggt ccagcgtaat ccgactccca cgctcgccaa
    24721 cgtgtgagga tcaggggtgt gccctttggc gttccaggcc cgtccgaaat cgacgaattg
    24781 tgcgaactgc agccggtcct ctccagacgc gaaccggagc agggggacgc gcgattcaaa
    24841 tgatgtcagc acggcatcgt cccgcacgag cgtgttttct cgatagccgc gtacgctgaa
    24901 gcgtccgccg acgggcattt gttcgagcgg aaagagccgg tcgttggcga tctgtgcggc
    24961 cacgcgtccc aacaactgga tgccccacca ttcctctatc cgtttgaccg cctgggcttg
    25021 acctaaccag gaaacaaatt gaccgtccgg cagaggcccg ttgttgacgg tagcacccag
    25081 gacgttcagt ccgacgctga accgggacag agcggcaaat acaaaggatg gggatcgatg
    25141 cgtatattcc tggctgaatc ggagcgcgct gacggtggcc accccggtcg tagaagaacc
    25201 gggaatgaag agcgacggca gacccgggac gtcgaacgca gacgtcgtct tcagatataa
    25261 atgttcgccg cctagagaga gcgcgacctc gtcgctcacg gtacggtaca caggttggcg
    25321 tatcgcgatc ccgatgactt cggagtccac attcaaatcc agggtgcgaa aaggattctc
    25381 gacgaccaga aaggcattgc gccgatagta ggccgtgagg gtcgtctcgt aggagttgag
    25441 cggcacacta taggatgtat cgatgatggg gttcacgcca tgcgaccgcc cgtaggtaaa
    25501 cacaaattgg tcgccgtgac ctgtcacatt ttggtgcgcc accgtcgcca ggccgcgctc
    25561 agcgccgacc accggtgttt gatagttgtt gaactccagc caggccttaa aggggttggc
    25621 gtctttgacc cgcagattca agacgctttc accacgaacg tctcccggtc tcagctcggc
    25681 attgatccgt tcgatgcgcc gatcctgctg cagcagttgg atccgttctt ggagaggctg
    25741 gatgctgacg ggagtgtcaa tgccgcgcgc cacacgatcc cgtaggtacg aactgcggaa
    25801 ccattggttg ccttcgacgt cgattcgggt gagggtccct tcgacgatct gaatgacgat
    25861 ggtcccgaag gtgacatctt gatcgggaat gacggcgccg gaggtgagat accctctatt
    25921 cacgtacaag agggtgagag ccaggcgaac ccgctccaga tcttccgtgg ttagttcacg
    25981 attgcgatag ggagcgatga cgtcagcgag ctctgcttca gaaaaaacgg tactgccggt
    26041 gaagtggatg tcatggacga acactcgaac ggatcccaat ctgttctcga acggcttgtg
    26101 cggggcgggt gacacttccg gaaggatgag gctcggaggc ggtgtttccg gtgctttgaa
    26161 ctcttccctc aacggagcag gcggtttccc ggaccggccg gtgggatcaa aaataggagg
    26221 aagcggcggc gcaatttgtg ctgtggctga agctcccatg aggcagatgc tcatcgtaat
    26281 gcctatgcac gcagatcgga tatgaaagat cccacaatgc aattcggctc cctcttcgcc
    26341 tgcgcgagag gctcacaagg tcaggagcga caatctgccg gccagtcacc ggcaccgagt
    26401 tgtggtaacg gaccgaactg atctaccggt tgttctgaac gcaccggcgg ctgatgcgca
    26461 gactcaaaag tgggcatctg tgccgattgg gtgaggcctt cgtcggcaaa catcaggggg
    26521 ctcatgagcc agccgcccgg ttccgcaggc agggcatccc ttcccgcgat cacgagactg
    26581 ctcaattgcc ccccggcctg cgccgcacag cgttgtcgta agagatgttg cgcttgtagc
    26641 ggttgttgcg gcagggtggc taagctgccg cttaaactgg acacgggcga ttgaatgttc
    26701 acggcaccgc tcaagccgaa ttgtgacgag gcactgatga cgctcgtcgg atcggccagg
    26761 aatgtccccg cgataatggt gatgttgccg ccttggccct gtaccgcttg ggcgaggatg
    26821 tgactgttct ggagcgtcac gactgccgga tcgagcgtga tattcccgcc ggatgtatcg
    26881 gggccgccca gcaccgaggt gctgagtcga ccgttgatga atcggatcga atcgatcgcc
    26941 tgaataacga tgtcgccgcc gctcgcctgg ttggcttccg tcgtgatgga cgcattttga
    27001 gcgaggaatc gaggcccgga attgatggta atgttgccgg cattaccaag gcctgtgctg
    27061 ctggcagaaa tggccgcccc gtcgctcacc gtgacggatt gagccgcgct cagcgagacg
    27121 ttgccgccgc tccgagttcc tctggtaagg gcggaaattc caccaccctc catcaagata
    27181 tttccagcgg atagatcgat attgccgctc tgttcctggc cgttcacgga ctgcggccgc
    27241 aaatccgtca cattggcgga tatattcgtg ttgtgcagtt ctatcgggcc ccttgcgcgc
    27301 atctcaattc tgcctccgag accaatgctg ctcgtgtccg tcaacacatc ggttcctgtc
    27361 aggcggatgg tagacgaagg agggtagggg acatcgtcag gaatggggcc actgacgccg
    27421 gtgatactga tcatccccgc accgccggtg ctgccgctgc tgctggaaat gaccactctg
    27481 gacggatcgg cgacatccaa tatcctgggt gccgccagcg tccctcctgc tctggttcca
    27541 ggtctggtgg tcaagacatc tgcgtgtaac tcaatgcggt tggcaatccg attcccttgt
    27601 gtgggggcaa tgatccctcc tcccgtgatg tccaagttct tcgcagacat gagtaggctg
    27661 ccctggacgc tccggccgtc cgtgacaacc gtgcccgcct cccccgcgcc cacgacatcg
    27721 atcaaccggc cctgagaatc caggacgttc gtcacattgt tcacttgtga gttcacgacg
    27781 gaatggtcga ggatggtagt gtcgcctctg attctgagat agcccggcaa cccttcgtgc
    27841 agcgcttcga ccgtgacctc cgaattggtc aacttcacca tatcgatggg agcatccggg
    27901 tacggggcat cccaaacagc gccgaatgaa aaatagtgat ccttgatgat cttggtgatg
    27961 ccggcctgag cccgtccatg tcccgtattc gcgatgaggg agttgtcggc tgtgaaggaa
    28021 tgttcggccc gcaaccagat ctcgcctgca ctcgggccgc cctgacagag cgggcatgac
    28081 ccaatgtccg acacctcggc attggccttg atggtactgt ctttcaagac aatatcccga
    28141 cccatgatat cgatgcggct gaagccctgg ttttgcgcgg actgcccatt gggtttattg
    28201 tcgttgatgg tgaagtcttg ggtcgtgacc gagacgccgg agtcgatcaa cgatgcctgc
    28261 ccgttgctcc tcatcatcac gattccaccc ggcgcgttgc cgatggcgtc ggaatgaata
    28321 tccgtgcccc gtaagtcaaa ctcgcgtgcg aaataatagg taatggctct gttgggctcc
    28381 gaatcagggg cggtgacgtg tctgtatagt cgcgtactcg gaggggtgaa gcctgggata
    28441 ccttgaagag taatatagcc ggcctgcccc ccgacacatt ggttgccgca gtgactatcg
    28501 cttgaaatca ggacgcgccc gtccggaccg gattgggttt tcagggtatc cacattcagc
    28561 gtgatcgcac cggcatccgc tccgcctgtg gtgtctgccc gtacggcggt cccgtttgcc
    28621 aactcaaccc gttgggccgt cattgcgatg ttcccgggaa cgaccgttgg gtcgacggca
    28681 ttggtgacgc ctgtaacaat gtgggtatgg ctcagtagca cggcatcagc cggctcccct
    28741 gcctttcccg caaaaaaaat agacccggct cccccctgcc ccgtactggt gctcgcaatg
    28801 gtgaccggtg cggcgccgct gagcggaatg ccatcggctc cgacattgct gcgcaacgtg
    28861 ctcacctcaa aggtgatatt ccccgccgta ccgtcgacgg cggaggtgag gacattcgag
    28921 ctgtgggaca gcgccacatt atccgcctgg acagagatcc ctcctgtcgc tgaagcagag
    28981 ggtggtgagg tcggggcggt ggccgttgcg ctcgcctcaa ggcgggcgtt ctccatggtg
    29041 aattgcccac tgcggattac gatgcgccct gcagtgtgag cgctcgtatg aacatcggct
    29101 ccttgagtga gtgaaatagt tccgagtgat gtaaacccgt tcaacgttgg gtcggcgggt
    29161 gccgccgccg cgttcgtcaa cacctcgccg gctgaagcga ctgcgataag gttgattggc
    29221 cctccggggg ctgaaagccg ggccggttgg attgtggtat ctgcgagggt tcctccttga
    29281 atggtaaggt tgcctcccac cagcgagatg ccttgccctt ccgcgaccgc aagctgactg
    29341 ccttgcacag caattgctgc cggattagac cccaaaaacc cgaacgcggc caccggtgcg
    29401 ctggagatgg ccgcatcctg cggcccggcg atcgcgttga atctcgcccc atctgtcaaa
    29461 cgcagatagt cagcggtggt gaaactcacg gatcccccga cattcagcgc ggcattcggc
    29521 ccaaagacaa tcccggcggg attcatcagg aacagattgg ctccgccgaa gccggtcgtt
    29581 tggagggtgc cgaaaatgtg cgagggattg ccgcccgtca cacggccgag aatattcgaa
    29641 gtgggcaacg cggtctcgtt caggaagttg gcaatgttgc tggtggggac gccgaattca
    29701 ccgaagctgt gaaagagatt cgacccgctg ccagggcgag tgccgccggt gatgtcgtac
    29761 acattgccgt tttgtgtgac cgtcgtattc aagcccgagg aggtgattgg ggtgggcgct
    29821 tgagcgtggc ctatgccagg cacggatgat tggatcagcc aactggcgaa cagtgtcgcg
    29881 cccagtaaca ggtgacgctc gtgaccggag ccgatccgaa atgcgctcaa catacagcct
    29941 cgctctatcg atcgtcggct tctatggcgg ctgttatgac ccacagccgg tttcccggtc
    30001 ccatacacct cgtcgagacg ccgaacccag atgatgagac atctgtcctg ctaacctctg
    30061 actttgttct ggcccgaagg aaaagccgga gacctgcggg gcctcttgag ctggcacctc
    30121 tggaaccatg aacgccatgg ggctcatcag ccacccgcca ggttcgctcg ggagtgcatc
    30181 ccgtcccgtc acgaccaggc tgctcagccg accctgtgcc tgcgcggcac agcgttgcgt
    30241 gagcagctgc tgcgcttgca gggggcgttg tgtcagcgtg gccaacgtgc cgctcaaact
    30301 ggacaccggc gattgaatgt tcacggcacc gctcaatccg aattgagagg aggcactgac
    30361 gacgctggca gaatcggcca ggaatgtccc cgcgataata ttgatgttgc cgcctgcgcc
    30421 ctgcacggct tgtgcgagta cctgactgtt ctggagcgtc acgactgccg gatcgagcgt
    30481 gatgttcccg ccggatgtca tcggtccccc ctgcaccgag gtattgagct ggctgttcac
    30541 gagccggatg gactcgatgg cctgaagcgt aatgtttccc ccgctggcct gactggcctg
    30601 cgtcgtgatg gaaccgttct gactgaggaa ctgattcccg gcattgatga cgatgttgcc
    30661 ggcgttgcct gggccggtgc tctccgcagt catattcgaa ccgccacgga tcgtaaccga
    30721 ttccgaagcg gccatggaga tatggcctcc cgtcccctcg gacgatgagg tactgctgat
    30781 ctttgtttgc tgatccgcct ggaacctgac tgtgtcgatc acaatgctgc cggctggagc
    30841 tccgccgttt gtatcggtcc taatgcggct attattcatg ctcaagctgt ctgccgaaag
    30901 agtgattgtc gcggggtttg tcgcggcggt tcctccgctc actgtcgtgg tgaactccgt
    30961 attgttcacg gcaatcaatt gcgccgaggc cgatgcggat tcgagtcctg cgatcgtgat
    31021 gtccccggat ttgccggctg ttccggtgtc gcttccgctg acggagaaaa tcgacgaggg
    31081 gggttggccg ttgatcaggg tcccgtcctg cttcacattc gctcggaatg tatgcacatt
    31141 gaatgtaata ttgctgcccg gcgcggagcg ggatgtgaag gcgaaaaata cggtccttcc
    31201 ggtcatgctg acggtgtccg atgtgatcct gatcacgcct ggaacgagcg ccgctgtccc
    31261 gcccaatacc gctgaactga gttgtgcgtt gttaagagcg atcaatgagg caggatcggt
    31321 agattccgga cgcaagccgg agatcgtgag cgccccggcc ggaccggctg tatcctctgc
    31381 gctcacgctc ggagtgttga ggaacacgcg atgagcgcct tcgataggcg tttcatcgtg
    31441 gttcacgttt acccggaggg tatcgacgtt gatggcaatg tctccggccg gtgctggccc
    31501 cagcgacgtg gtgaccatgg tggctgcgcc gatcccctgc gcgtcaggga aatcatcgtt
    31561 ggtcaggagc agcgacttcg tggtgagtgt aattgcggcc ggtctgctgt cggctgatcc
    31621 gccgaagatc ctcgtgctga gcgaggtgtc tttcaacgtg acgctcgtcg cggcagttcc
    31681 cattccgtcg accccttgta ccgtgatatg ccctgcggag ccggcatcgg tcgcgaaact
    31741 tcggctatcg ctcgtcacca ggttttgagc gatggtagac ggatcatttg gattcaattg
    31801 aagccgattg gtggcatcgc cttccgtcgt gagggtcctc acgttgaatg tgatgtcacc
    31861 tgccggtgct gacccgtgtg tgtctgcggt aatgagtgtt ccgttgttga gtgcaatggt
    31921 ctccgacgta atggaaatgg cgggtgatgc cgtctccccc tcggttacca taccgtgacc
    31981 atttcctgtg attgatttga tcgaggcgtc atccatggtg aattgcccgc cgcgaatgat
    32041 aattcgaccg gctttggtgg cgctcgtatc aatggtcgtt ccttgagaaa gagagatggt
    32101 tccaaacctg ctgaatccat tcacaaaagg ttcgaggggt tggttctcta taccggttgc
    32161 cgacacttct cccggcgagc cgacagagac cagggtgatc tgccctccgg gggctgaaag
    32221 ctgggccggt tggattgtgg tatgtgcgag cgtccctccc tgaacggtga tgttccctcc
    32281 gaccaacgag atgccttgcc cttcggagac cgcaagctga gcgccctgca cggtgatcgc
    32341 ctcgggggtc gatcccagaa acccgaatgc cgctaccggt gcactggaga tagccgcatc
    32401 ctgcggcccg gcaatcgcgt tgaacctcgc cccatctgtc gaacgcagat aatcagcggt
    32461 ggtgaaactc acggatcccc cgacattcag cgcggcattc ggcccaaaga caatcccggc
    32521 gggattcatc aggaacagat tggctccgcc gaagccggtc gtttggaggg tgccgaaaat
    32581 gttggaggga ttgccgcccg tcacacggcc gaggatgttg gaggtcgcga ggccgctgtc
    32641 attgagaaaa ttggcgatgt tgttggtcgg aacgctgaag tctccgaagc tatgaaacag
    32701 attggttccc gctcgcgttc cggcggtgat gtcatattgc agggagtttc ctgaaggctt
    32761 gctgatgtgg gtattcagtc ctgaggatgt gatcggcggg gtgacttgag cgtgactcgt
    32821 gtccggcagg ccgagaggaa tcagccacca gcaggcgaac atggtcgcgc agacacaggg
    32881 cagccgagtt gcgccgccgg cgaagagtct gctgcccctc atcattttca tggcaattgt
    32941 gcatctcagg tgcaagcggt caggactacc cggcccattt ccgcacggtg tagggcgact
    33001 ccattcgttc agacctcgct atactctacg cgattgcccg gccgcatagg tcgatcatgt
    33061 gggctaggga aatactctcg accggcaatc cggtgcaatg ccggtttttg gcaccccatc
    33121 ggcacgagcc tctcttcccg ccgtatacag ccatcttcac agcccgcgca ctactccgac
    33181 accctgccgg gacctgttcc ctggttacga agaggagtta gggttcttgg acatctcttt
    33241 gcgtgagcga gactcattga ggtgatccat tcccgtcgaa tcggtcgctc ttgaccgaag
    33301 agggcttgga tgcatccaaa tgcgtcccac agctgcgaga aggaagacga caccgagtag
    33361 gagaaaaaga agcacgagca tggtgacctc atggatccgc tgttctcccg ttggcgtact
    33421 tcctagggaa acatcctatc tgcctcgtgg atgcgcaaag gttgtgccat ggagggtgac
    33481 ggaaagcatc ggtgattttc gatggcaaga tgcgtagtga ttttgatgac atccgatatc
    33541 acgatcagtc tcgttcttct ggagtagatg tacgtactgc cgtaaggtgt atcgaatcgg
    33601 atgctatgcc gtatccaatc tgatacgaaa tgatcgcttc gatgaatgtg aaacgatacc
    33661 gtccacacgg gaggacgatg ccggacgatg agatcgtcca atagcaggag aggagggtta
    33721 cactgccggc ctggagagag gttgccgcgt ctcctgtgat ggcgaagacg cggcagatgg
    33781 cgtggagaag gcggtctgat cagcgctgga tgggcacgat ccaggaggcc gtgtcatgcg
    33841 gttgctcttg cttgacaacc ggcgacagga aaccggctct ctgccacgcc gaaagggcac
    33901 gagcacgatt cgatccacgc tgttctctcg actgcattga cccatggcag tggaagaagc
    33961 taccgtcatg ccgtcgatgt ctgaagatga ggccatcacg gcgaatcgac gaacggttgt
    34021 cccgaggccg aagggcctct gccgtaggcg tgcggcatgt tgtgtctgaa gggaagaaac
    34081 ggggcatccg gacataacga tcggagccgg caggaacgtg ttggactcag taattgaacc
    34141 gcaaggacag cgagccgaca tgaaccatgc tgtcgtatcg gccattgacg gttgggtttt
    34201 ggttgcctgc gatggtgcgt acttcataca gacccgcttg atacgcgaga tcgatggcga
    34261 acaatttcgg cttcagcggg ccgattccca gttcgccgca acgcaccagc ccgaggaacg
    34321 aaccgttttc atggcaggcg aacccgatcc cgacggaggg aatgtgggta ttggctgagg
    34381 ggactcccgg attaaaggtg cgatcgggta tttgtgcctg ctggttcatg tagccggccc
    34441 gaagggcaat atcccaatcc ggcatcgagg ccaggcgcag ccatcggtat tcggtgccga
    34501 tcatgacggt ataagtgctc tgccaatttt gcggaaacgg caacagggcg ccgttcgtca
    34561 aatggacatc gaggttcctg ttggatttcc atcccgtgta gtccacatcg aattccagtt
    34621 tccactcgcg ctcggacgta cgaaccggcc agatgccgat gccgccggag aagacttgcg
    34681 gcaggacgaa ggtggctgtg gcgccggcga ctgaggcgcc gttggcgaga aagttgcccg
    34741 tgagatgaaa ggtggcttga ctgcgataga ccatgccgat gttcacgagt ggtttcccgt
    34801 cgctgttgcg gaagggggtg tacaggaggc tgacgttaaa tcctgccgtg gtgtcgcttc
    34861 cgttgatctc catcagggag ccggccggga tgcccagccc gccgggccag atggatttct
    34921 gttcgagatg gccttctccg aagagcccgg aaaaggtgta aatatccgcg cccaatccga
    34981 acgagagttg gtcgttcagt ttgtaggcga tggtgggttt gatatcgagt aacggcaagg
    35041 tggtgaaggt gatggccgtg cgaaacggcc catcgttcgg atatctggtc aatgaaccga
    35101 acggattgtt catcccgata ccggcggtga agttcccgag tgccgacagg ccgaggtcct
    35161 tgagattcgc gaccaggtac acgtggccgg gagggggcca ggccacgctt ccgttgcgat
    35221 cgcctgtcac ttgtgcgccg gtgggactcg tgaactgggt ggtgcccccg atcagcgtgg
    35281 ttccgaacag ggtttggaat ccatgcaatt gtgtcatgcc ggcggggttg taatgaagcg
    35341 cggaagggtc atcggcctga gcgacgaagg cgttccccat ggcggcggcg gcagcggact
    35401 ggccctggag ccgtggggtt tgagcgttgg cggaagcggc agcgaagacg aatccggcgg
    35461 ccgaacacaa caggcagaga agcagggcgc ggcggaatcg ctgagcgaga ggcatggggt
    35521 taccgtccta tcggagactc ggattgaccg gagccgatga cgaaccaacg atcgacgatt
    35581 tgccgcagcc tggttccatc gaagggcttc aggaggtaat cttgagcgcc ggattccact
    35641 gccgccatgg cgcgatccag cgcctccacg gccgtcatca tgaccaccgg caacgtgggg
    35701 tgagtgggac gcaactgtcg caggacttca aacccgtcca attccggcag ggcgatatca
    35761 agcaacactc catcgaccgt cgtctccgcc agcatccgta atgcggcgcg accgtccgcc
    35821 gcggtgcaga catgaacgcc ttccgcctcc aggcgatccc gcagcaggtc acaaatgtca
    35881 ggatcgtcgt cgacgaccaa caccgtgcgg cgaacggcgg gcaactggcc ggcgggagat
    35941 ggttcgcgcg aatgcagggg aagggtgaag gtaaactccg ttccctgatc cacctcgcta
    36001 cgaaccgtga tgctgccccc gtgtagatcg actaagtctt tgacgatcgc caacccgagt
    36061 cccaatccct tggtttggct tcgctcttct tgctgcacgc ggaagaacgg atcaaagagt
    36121 ttgggaagcg catccggggg gatgccttgg ccgttatcac ggatgacgat acgagccatg
    36181 tcgtgcgtat cgaccgaaag ctccaccgag acctgtcccc cgtcaggagt gtacttgata
    36241 gcattatcca ataaattcgt caaaatctga ctgagccggt cctgatccgc ccacaccagc
    36301 agttcgggat ccggactgtg aagttcgata ttgaggtgct tggtgatggc gagcggcagg
    36361 agttgttcga tgacgtcgct cacgatcgtc ggaaggcaca cctgttcgaa agagagcacg
    36421 agtttaccgg cctcgacacg cgagaggtcc aggaggtcgg tgatcatgcg ggcgagtctg
    36481 gtgccgttgg ctttgatgcg ggtgaggtac tgctcctgct tcatgttgag ggagccgacg
    36541 agtccctcga gcatattgtc cgcgaagccg acgatgctgg tgaggggcgt tcgaagctcg
    36601 tgggacacgt gggcaagaaa tttcgatttg agccgatcca tctgcttgag ttgcgcgttg
    36661 gcggcttcca actccgccgt acgctcgcgt acgcgggcct ccaggccggc attcaacgat
    36721 tcgatttctc gatacgcctg ggcattgtcc agcgcgatgg cgacctggct ggcgagggtg
    36781 accaggacat cgagatcgtc ttgggtcagg gcctgattgt gtgtgcggtc gaccgagaga
    36841 gagccgatga cggcatcttg tgtcttgagt ggaacggtaa tgaacgattt tacattcacc
    36901 atcgcgatca atttttgatc gaaggggtgc agccggtccc agatttcgtg aatgttgttc
    36961 gtcagcacgg gctcgccacg taggaacacg gtcccctcca cactgttggg gtcggagacg
    37021 ggcacttcct gcgtacgcaa aaaatccgcg acctcctgcg gcatgccgcg cactcgaaag
    37081 tcgtgcgaca cgttcctcgt gcgatcgaac tgcgtaatca tggcgcgatc gtagtgcaat
    37141 tcacggacga tcgtctctaa aacattggtc agcagctctt cccgatcgaa ggtcgaactg
    37201 aacaacagtc ctgcacggtg aagtgtcgtc aggtcattga tttttcgtcg taaggtgacg
    37261 gcggtactct gttgttcgag gtaggcctcg cgcaattcct catgccgcgc atcgacgacc
    37321 tgctcctgat ccttgatcaa ccgttgcagc ggtttggcgg cttgtcgcat gtgattggtg
    37381 atcatccacc acaacagcga cgctggaata gccgcgagcc ccagcgcttc caccgtcggc
    37441 acgtcaggct ggcgaagatg caggtaggtg aaggtgcctc ctgcgacgag catccacatg
    37501 gcgagcggca cggaactacg gacttgcggc gtccatgtga attcccattc gcaataggga
    37561 tcgccgttgg cggtgcagga gaggtccctg accgtggcgg ccggtgtgcc gtgcatttgc
    37621 gtctgtaccc gcgcgatact gctcttgcag gagaggcaga ttacatccac gcaccgcttc
    37681 cggtagggac cgaattgcac aagggcccga tcggtgaagg tcatgcggag gaccgccgaa
    37741 cgtctggtca cccggccgac ctcgaactcc aacacacccc ggacgtactt gctgccgaaa
    37801 tagggccaca ttttgtagag ctgtggggtc gaaaacggac gacagaggat ttgaatcaag
    37861 ggggggactt tcctgttcgt tcccacgttg acgtgaaatt gaggatcccc ggacaattgt
    37921 tcgcagaact ctctgaggta acagacgaat tcgtaggagt aactattcca gatattgcga
    37981 agcgagtcgg gggtgacatg gtaggtgcgg tccggaaggc gggcgttgag caatcggcac
    38041 agttcatcca gagcctgagt ggcggcggct ttcccggcgg tgcgactcag atagtcgcac
    38101 atgtagtcca cattcgattg aacgatgacc ccgctgatgt cgcagatctt ttcgccttgg
    38161 tcgttgaggc cgaaggggcg aaactccatg aacggtcgtt cgagaatgga gtgttctttg
    38221 gagagaaggt cggtcatacg gaagccgccc gattcggtcg atgccgcgtg acagaggagt
    38281 gccccggcgg ccagatcata acgtacctgt gctcgggagg cgaggccctc cgtgcagcgt
    38341 cgcgtctgtc ggtgcgcgcc tggtcacccg gctgtgatcc gttattcaag atcccggcag
    38401 gccggggcat catgtatgac caaacggtcg aggtcacagg tcttcaagta ttcgacgatg
    38461 gcccaacgat cagcgatcgg tatctcacat cctaatacac cggcgcccgc caccacagac
    38521 ccacaatcag accctttgaa ctcatgccct tccatgctgt ttcccggaag gtgagtttta
    38581 aattcaaatc ctaccaatgg atcgcggaag gtcggagaat cgttgcattc cgagacgacg
    38641 aacccgacct tcaccggatc gaattccatg ttcggactga gatagaagca ggccggccgt
    38701 tccttaaccg gagagagcaa tgcgtagagg ttgggaacgg acccattgtg taggaatgga
    38761 gcggttgccc agacggcgag atgcgggcgc gcgatgtatt gcgccttgtc acgccatgtg
    38821 ttggtgagat gctggtactc agggtcgctc cccgcgccgt tgagggtcat caattccgtg
    38881 gtgaccaact gggaggcgtc ttttgccgat aatcggccca acccaagacg ttcgaggtca
    38941 acggtacgtc gggaaaaatt ctccagatac aggctgtccg tgccgacctc tttctggggg
    39001 atcatcgtga cgtgcaggct tgggccgttt gggtttggcg aatcgagctt cgtggcgaca
    39061 tggcaatggg cgcagagatt cggaaggccc ttcagcttgt tgccgtgata gagatctttc
    39121 cctcttgcag ccaactctcg attgatcgga gggaagctct tcggccacct tggtgggtgc
    39181 agccgtttcg caagcccctc caatgttttc aacgaggcca catcaataga cgaccgaaag
    39241 atctccttct tttctgaagg aggctgcgga gaggttcttg cccaagagaa gagtgcggcg
    39301 ttcaccccaa tgacttgggc gatatttctg gcgagcggat gttgaatgga gccggcccac
    39361 tgcacccaat catattccca tactccccag agcggcggaa tactcaccgg cgcattggca
    39421 ggtctgaggt tgtccggatt gagcggtgag aagaccgtat tgccgccacg accgagcgca
    39481 tcgaaccgtc ccgggcccca cagtgctggt gaggcatctc ttccccctct agcaacgaga
    39541 tccttcatga cctgtgagaa ttggccggct aaggtagcga tattctcttg agttaccgcc
    39601 tgtccacggc gttgcagtac acgagctgca aaggcctgga atttgtcggg ggctttcaac
    39661 tctccaagac tctccaggag tacttgtagg aaccgctggt tgtattggag ggacggaccc
    39721 ccttcgatac gaatcgggtt gccttggtag gtgaactgag tcgtgtggca gaacgcacag
    39781 gtcaacccga cagtcggctc cgggacgttc ggcccctcgg tcttcgtaaa tccgattgga
    39841 agtctgtcgg gatgggtctg gtcggggatg atgccggttc gggcgaagct gttcttgagt
    39901 ggctcgtcct ccaggacgac aaaccaatca taagggagca gctgggtgcc ggcggacgta
    39961 tggtaccact gttgccggtc ctgctccgtc catccttggg tgtcggatga actgactgtg
    40021 tggggagggg gcagatcaat cgcctggcaa tgtacggtcg agagtgcaaa cagcagcccg
    40081 gcaagtacca cgatacgttg ggagtagaaa ggcatggtcc atctcctcat ggtgggcgag
    40141 attatctgaa cgggtagtgc atgattgatc cagaggttgg tcaacctgac gcgcatgcat
    40201 cattcattcg ctgattgcga cggcactgat ggaggcaagc gccgcactca ctcaggtgct
    40261 gagaagcaac tcatatgcca taagcgcggg aagggtacct ggatcggtac acgaaagcca
    40321 tttatttttt gagcgcgatg cgtggctggt ggcaggtttg aaacccgtct cgtccggtat
    40381 ggaaagggaa gagcgggtca agcgcatggg gtatctgaag agatacagcg cagttgatga
    40441 aaacgatatg cggaagaagt gaacttgcta ctggtggaga tgtttcatgg cagcggcgca
    40501 gaggatgatg aagaagccca tccagagcgc ggcgcgttga aacggaatcg attcgtagtt
    40561 ttcgccttcc tcttcctcgt cctgatctga cttgaaaatg acggtgtaga gaaacagcgt
    40621 gagcaggcac aagaccagaa tcagcttgat gcagaaaatc cagaggtact tgggatggtt
    40681 ctgcatgccc tgaccggtct gcgagacgaa gatctgattg acgtagtgga ggttgatgcc
    40741 gcccgtgaac agcaggacga ccaacaggat ccccgccacc cgcttgtaat gcttgtaaaa
    40801 ggcgtcggtg atcgggcgga catattcctt gggtagggcg gcttctcgaa gccccggctt
    40861 gacggccatc acgatgaacg cgaggccgcc gatccagatc acagctgaga gaagatggag
    40921 ccagtgattg acgatcggaa tcagttgatt cagatcaagg aagatacttt gtacggaatc
    40981 catttataga tcggtcaggg gcgaccagcg aatgtagctg gggctaccgt aggtcctgcg
    41041 tggcgcccgg ttcctagtcc ttccctcgca tatcgaaatt gaagaccggc gcgtcgtttc
    41101 caaatcgctt tttccgcagc tcttccatca actcaacggt caccatcgcg attccggttt
    41161 ctctcgcatg gcgctccacg cccttcttga ccatggcccg caggaaatag gggattcggc
    41221 tgagtcgaag cgccgaggcc tcgtcccatg caatatcggt ctcttccgga gtgttgaaag
    41281 cgaccttgat cttgtctccg cccttggggg tgtataggca ccattggtct tcatcgagcc
    41341 agtcgccgtg atctacgtag ggtcgagccc gacagccgcc gcagatgtcc gtgtactcgc
    41401 aatcgccgca tttgcccttg agttgggggt agcggaagga attgaacaca tccgagcgtt
    41461 cccacaaatc gacaaaactc gtctcgcgaa catttccggc cgatagcggc atgtagggac
    41521 agggggtcag ttccccgttc ggagtgactc gtgcgtaatt cgtccctgcc agacagcctc
    41581 cgcccatata tccggtggct ttggtcagag gggaattggg atctttttcg taggccagtc
    41641 gcttgaaatg cggagcgcag cgggcgcgta ccagcatatc tttgtactgg tcctggcaat
    41701 ccacaagata gccaagaacc tcttcgtact gttggggtgt gatatccgtc agttcttcgc
    41761 cgcgtccggt gcagaccatg aagaaaacgt tcaggacccg tgcgccgagg gtatgggccc
    41821 agtccacgac tgcaggcaat tcctgatagt tcatgggctg cgcactgaag tgaacctgga
    41881 actgcagacc attgcgtttg caggcctcga tgccggccaa tgcgctggca aaggcaccgg
    41941 ggacaccgcg gaactgatcg tgcttgtccg gatcaagcga gtcgatgctg atgccgacgc
    42001 ccatgacacc gatctcgacc agcgtttttg ccatgcgatc gttgatcaac atgccattgg
    42061 tgccgaacac gacgatgaag ccgagcccca cggcatgtcg ggcgatgtcc aggatgtcgg
    42121 gacgcaccag cggttctccg ccggtgatca ccagcaggca tcctttgttc acctcggcga
    42181 tttgatcgat gagacggaag cattcttccg tactgagctc atccgacccg ccgccggctt
    42241 tggtcgtagc atcgagatag cagtgatcac acttgaggtt gcagcgcttg gtgaggttca
    42301 gcgcgaccag atagggttta aagtcatcga ccgtgcgacc gtcgttcggc gcagccgagg
    42361 gggaagtcgg ggagatgaac ttcgagattt gttgctggag agactccagc ttatctccga
    42421 ggaacgggaa attgagaacc ggcagtgact ttcccatcac cggttcgccc cggttttgct
    42481 ggaggagccg gtcaggttgg cgatcatatc tttcagattg ccggtcttca tggcatcggc
    42541 catgtagccc ttcgcctgct cgattccctc agttgccact tccagggtga tgaccgtcgt
    42601 gccgatttta tcggcatgtt tttcgatcag ggccttggtg cattcccgca taaacccttc
    42661 cggtgcgcgg tccaaacgac cctgagcatc cggcgtccag gtatagggcg acacggtttc
    42721 ctgaggaacc ggagccggtt cttcgccatg gccgttcttg gcatgagcgt cggctccgtt
    42781 ggtttcggct gccgcaggcg ccagttgagg agtggtcccc gtccccttgt ttgcaaagat
    42841 aggggtatag tcttcggttg ccgcctcttg aatcatcggg ccggcatatt ccaacgtgat
    42901 cgtggtcatg ccgagcttgc gggcactctt ttcgatcttg gctttggccc ggcgacgttg
    42961 gaacccggcc gggacggcgc gaatcgcttc cttcgcatcc ttggtccact gcatcgggac
    43021 atcgtcgtag gcgagatcgg tctcaagccc accctcggcc ttggcggccg cttcgaagat
    43081 cgtcttgtcg acttgtttaa agcgatcccc ttcggcgctg cacaccgggc atttcaccgg
    43141 agtgtcgccc tttccgatat atccgcaccc gtcgcagaca aagtagttct ggctcatgcg
    43201 atccagatag gcctgggtac cggggctgtt gaccggcttt tcctcgacca cttgggtcat
    43261 catgccgctc aaggtggaac ccatcaggtc ctgtgcgact gaatcgtcag cctgcatctt
    43321 gtcgcgatcg attccggcgg catccagatt tccgcccaac gcccgcatcg catccatcgc
    43381 tcccttgggg agaatatgtc cgacggcaga gtcgacgacc gtattgctga taatcgtgtg
    43441 gcccttctca atagcgtacc ggtgaatggc ggtcttagcc actccgcggg caaacacggg
    43501 aatccgttcc atccggcgca gggcttcttc ggtccaggca atggtgtatt ccgcctgcgt
    43561 atcgatcggt ggcacatact tccgattgga gaccagaata ttgcacgacg cggcccgaag
    43621 caggttttcc gtgttgctgc cgatatccat gtcctcgtcg ctgtggacgc cgatgcgacc
    43681 cacgatgagg agccacggat tttctttccg cacgtattga atgatcttct cgaaggcctt
    43741 cccgtccagg agggtcgttt tcacatcggt ctgctcggcc tgagcgattt cacgggagat
    43801 atctaggtgc gactgataaa tctttgccag tccgctgtcg atgacttctt cgtgcagctt
    43861 ctcctgctcc ttgaagcgga agaccttgcc cgcctcttca ttgagcacgc cggaaatact
    43921 gtgaaacgcc gcatagtgga agtaggggtc gaaggcggag atggcttcca ccggcacatt
    43981 aaaggctttg cccagcgcca aaccggtcat aagaccgccg aaggagtacg gactgccgtc
    44041 cacggccacg acgatcttgc cggtgcctac cggagtcggc tgcttgatga tcaacatgtc
    44101 ggagttgcgc acgcgtcgca ggacccgctc ggtgttgctg ccgatcacgc tgtctttgac
    44161 cgctccgact cccaaggctc ccatgatgac gagatcatag gcattggtat tgatgtcctc
    44221 cgccagcact ttccagttgc gaccttctaa ggagcggcgt tcgacaggca ggttcgcctc
    44281 gttacacttt ttatccacgt aatcgaggta ggaatccgtg atgatctgga gtccgcgggt
    44341 aatcagcgaa tcgtggatct ggcgctggcg gtcgagttcc ttctcgtcgt gatattcctc
    44401 gggaaggccg gcctccatct gcttaaaacg cttgtcgtgc atcttggcgg catagacgtg
    44461 actgcccaca atcttcgagc cgtaggtctt ggccaattcg actccaacgt cgacggctgt
    44521 attggaatgg tcggaattat cgaccgggat ataaatggtc ttgtacatcg agacgcctcc
    44581 taggcaaaca ctctataggg ggatgagatc agagaaccgc caagaattca aacggttgaa
    44641 tggaacttta ccccaaaaga agcggcatga tagcaccgtg ttctcgggga atgcaagcaa
    44701 accgagggtg tgcaagggcc tgtgaataac ggctaactta ttggactcac agccgtcttt
    44761 tccgtggtgg cagtcgtcgg ctgagccggg cgacgcgagc gcagtatctc ctccagggtc
    44821 aacaatgcgt cccgtccgga gttgttttga atgccgcgaa tgagattcgc atctgcatag
    44881 tgggccgtat cggatttcgg gccggaggca gtcgcttgag tagtggtctg agacagtgat
    44941 ccggtccagg ctcgaggatc gcggctccct tcttttcgct cccacgcggc gaggcaggcc
    45001 ttggcgatga tcttgttcaa cggcgccggg ttatagagga gtttgcgaat gcttgtcggc
    45061 gtcagcatca aggggttgta ccccgccgaa aggtaccctt cttcgagcag ccggtgttcc
    45121 aggtccgtat tgggctggat gccaaggaag aacatcaagg ggaagacccg ttcctctccc
    45181 aaaatcgcgg cgaccttttt ataggattcc acgctctgga gcaggctctc ttccgtctcc
    45241 ttcggggagt tcagagaata attgaggatg accttgccct tgaagccggc ttccgccaga
    45301 tagcggcacc cgtcatagag ccgttcgagt ttgaagccca tgtgcaggtt attcagcact
    45361 tcctgcgacc cggaggtgat ggcgacttcc aaatctccga cacccgaccg caccatcaac
    45421 ttggccagat ccggcgtgat caacgaggtg cggatgtaac cggaccactc gatctccagt
    45481 ttttcactca aaatccgttc gaggatctcg gtgcactgcg gataggcttc cttcccggta
    45541 atgaactggg catcggtaaa ccagaaacgc cgggcacccc attgatgata gtgctgagag
    45601 atatccttga cgaccatttc cggcggccgg taccgcacgc gtttgccttc aatataagga
    45661 tacagacaga aggcgcagtc gtacggacag ccacgtttcg tctgcacgcc gattgattca
    45721 tccatatagg cggcgtgctg agggaaaatg gaggtgagat agggcagatc gacggtcaag
    45781 gcatcgagca gcgctggcga gccctggttg cccttgcgcg tctgtttccc ctcgcgaatg
    45841 atatagcgtt cgttttccag cgattcgccg ttaaccacct tcaggatcgc gtcttcccct
    45901 tcgcctaaaa tgccgatggt cccttccggc agtttttcga tcaactggtc ggcaaaggcc
    45961 gtgaaggctc cgccgccgat catgatctgc gttttaggga attccttccg gatcagccac
    46021 gggtacgaca ggttggcgcg aatatgactg taatagcggt agagctgctt caacccggcg
    46081 aacgacgcag ccacccgctt gatggggttc ccggcgaaat aaaaattgaa cgcatgctcc
    46141 agagaggcat cgccttcgtg gggagagaag atctgaatgt cccgccacga aaagcagacg
    46201 aggtccggct taaactcaag cgcggcatcc cggatcgcct gactgcgttg cggctctgaa
    46261 aacaacgaca ggtcgagaat acgctgccgc acctccggtt tccggcgatg gatgaagtcg
    46321 gccagatagg tgaccccgat cgggtacacc ttcttgcaag ggagaaaaat gtagagaatg
    46381 gattccatgg tcggagctga tggctaatag cgtatggcta acaggtgaga aaggaattgt
    46441 tccctgctcc tggtgctatt cgccataggc tatacgctct ttgttacttg gttgcgatgt
    46501 ggatggcgac gatgcccccg gtgaggttcc ggtattccac ctggcggaat ccggcttcgc
    46561 gcagcagggt ggccagccgt tcctgatcgg gaaaggttcg gatcgaggcc ggcaggtatt
    46621 cgtagacgcc agtccggtcg cgcgcgacct tggtgccgat ccagggaagc aggcgaaacg
    46681 aataccaatc gtagaggccg cgcagccagc cgtaggccgg tcgggaaaat tccaggcaga
    46741 cgaatcgacc acccggccgt aggacccgcc ggatttccgt gaacgcctgc gtcaggttgc
    46801 ccacattgcg catgcagaag ccggtggtca cggcatggaa ggtctcgtca gggaagccca
    46861 gctgctcggc attggcctgc aggcaggtaa tccggtcgcg gagcccgcgc ccggtgactt
    46921 ttctcagtcc ttccgccaac atcgcatggt tcaaatcgga ggcgatgacg tagccctgct
    46981 cgcccatttt gggttcaatc aagagggcga gatccgccgt gccggcgccg atgtcgagtg
    47041 cgcgtccgtt ccttacggtg ggcacgtacg acgcggtgag ccgtttccaa tggtgatgaa
    47101 ggccgaaact cagcagggtg ttgttgagat cgtagacgcc cgcgatcgcg gtgaacatcc
    47161 gttggacggc ctgctcgcgc gcttggccgg accaggtcga gacggcttgc ggctgtggtg
    47221 acggccggtc tgtcgcgggc gtgggttgtt cttggacctt cagcataagc tcgtggtagc
    47281 accagctttc aagctttgtc aagccggaca cacgggatac taattgagga atgtgacgcc
    47341 tgctgatagg aaagcttcct ggtgaaatga gacgagggaa atccttgttt ccattaggag
    47401 gaagggttat acgtgcttcc aaattatggc acgcagattc tttcgctcct ccatacaaga
    47461 gctggaagcg ttgtttaagg agggaaacga caacgtagat attcttcatg cgttgcagga
    47521 ggagctgaac catcggaaaa gcgagcgggc aggaagattg cgcacgcagg tcaaagagag
    47581 acttgcgatc ttgcagcccc gagcatctga gaagaagcct gccccagaaa aatcaccatc
    47641 acgccgatca cctgacgatc aatcgggatc tcgctcccgc gaaaaatatc cgtcgcccca
    47701 gtctcctccg gcttctcctg aaagcccctc aagagaaaga tcctcgggaa ctgatagcga
    47761 acccccgcca ccacccatga ccaatcgacc ggacgatatt ctttcggcat ggaccgcaat
    47821 ggaagttctc tcgccacagg tctatatccg gccagaagac ctagctgctg gtgacaagag
    47881 gcgagtcact accctaagcc taagcgagcc tcttccgtgg gagcgaggtg acaagtcgct
    47941 gcccaaatat cgtctttatt atcaggttgt tcttggctca ataaagatgg aatcagcgat
    48001 cggttgcctc gttagcaaat acagtgacaa tcgagaagat cctccgcctg tgcgtgggag
    48061 ggcagcgctc gccactgtcg tggtagatcg ccatggccaa cctattgagt cgccagcagt
    48121 gggaatatcc agttttggct ggggtgtgat agctgcgatg aaaggtgagt tggcagacct
    48181 cgcacgatgg acagatgtcg agtcgcagtt ggttgaacgg atcgagaagc tgcttctagg
    48241 aacaacgacg ggagatgatg gtggaggtga gcgtcgaaca tatccattga cccgggctgc
    48301 gttgtttgct gcttatgagg cgcttgttca ggaattgggg cttccaacag aatgggttga
    48361 gccgccggag ttcgcaatcc ggtcatatgt ttatttcaag gactccaatc cacctgagcc
    48421 gctcttgctc aatagtttct ttctgccaga ccttgggacc gctcgtaagc agtttactga
    48481 agctaaagcg ccaaagaatc ttaagcgcta tctcggtgtc gaacgcccac agaataggat
    48541 agatttactc aataataggc ccgcattggc ggaagctatt agccctggac ttactggccc
    48601 atcgcgctgg cctggcgcag gtcgctcccc cctcgtccta cttcagcaag cggctgtcaa
    48661 cctggcgttc caagaaacaa aggctggtgg cttgctcggt atcaatgggc cgcccggtac
    48721 tggaaagacg acgttgctac gagatctggt cgctggagtc gttaccgaac gagcagaggc
    48781 aatggcgaag tttgatgatc cagaggctgc gttcgagcat tccggggaaa agctcagggc
    48841 tggtgcaagc tggattcacc tctatcgcct gaatccaacg ttgcgtgggt tcgagatggt
    48901 tgtggcgtca tcgaacaaca aagctgtcga gaatgtgagt gcagagctgc cgaaccttag
    48961 cgccattgca gaggatgctc cagagctgcg ttacttcaaa acattgtctg atgcattcca
    49021 gccagctgaa ccttctgaga cctggggagt cattgctgct gtcctaggga atatgcagaa
    49081 ccgaagtcga tttaagcagg tcttttggtg ggacgaggat aacggactca atgcctatct
    49141 gcgggctgca acgggcctgg acagcgatgt gaagataact gatccggtta cagggaaagc
    49201 tcatcaacga cctgcgcata ttgttcaaca ggagcagccg ccatccagcc gagaggaagc
    49261 ccttgaaagg tggaggggtg cgcgttcacg atttcttcac gtgctggacg aaagccgtcg
    49321 acgacaagca tggttggaga gcctttgtgc tgagttaaag cgattgcctg agttggctca
    49381 aaacgaaatg aacgtaagat ctcagcggga tgccgcgtcg gagaaggttc gacaattgaa
    49441 ggggctctcg gcagaagcat gggaagtcgc acattcatct caacgcgcag ccatagaact
    49501 gcagctgcac gacttatcga tgccagggtt ctgggcacgg ttgttcagaa cacgtattgc
    49561 tcgtaaatgg aatgagatca gacgagcgct gctcgaatgg caaaacgctg aagtcgcctg
    49621 gcaagtggct tgtgcccagc atcagcaggc gaatcaacgc ctcgttgagg ctcaacagaa
    49681 gcacggggtc atcctgacag atgatgtttt cttctcgtta gaacacagta agcgacacca
    49741 agcaacaccg tggttttctc cagctaccca acgtctgcgt gacgaggtgt tcatttctgc
    49801 gatagcgatg catcgtgcct tcatcgatgc ggctgccaag cctttgcggc acaatctcgg
    49861 cgctttgatg gatgtgttca cgacgcagac cctgaagggc gaagagaaac aggcattgtt
    49921 gtctgatttg tgggcttcat tcttcctggt ggtgccgttg atctccacga cattttcgtc
    49981 tgtcaaccga atgctgggca agttgcccct cgaaagtctc ggttggttgt ttgtggatga
    50041 ggctggtcaa gcattgccgc aagcagcagt gggcgcactt ctgcgaactc gccgagccat
    50101 tatcgttggc gaccctgtgc aaattgagcc agtggtgatg cttcctgata cgttgacaca
    50161 tgctatctgt cgccgctttg gcgttgatcc caaccactat gcggctccat cagcttcggt
    50221 gcagacgctt gctgactccg cgtcctccta taccagtgag tttcaaaccc atgttggaag
    50281 tcggaatgtt ggtgtgccgc tcctggtgca tcggcgatgt tctgagccga tgtttagcgt
    50341 ctccaacagg attgcttatt ccggactgat ggtgtctgca aaattgccaa agccttctgc
    50401 gatccgagag atacttggtc cttctgcatg gattcatgtc gaaggcagcg gtcaggataa
    50461 gtggtgccga gaggaaggcg atgaagtgat ccgtatgctt catcggctaa gagcagccag
    50521 tgctaagcca gacctttatg tcatcacccc tttcgtcata gttgcagacc gattgcgtca
    50581 aacgatacgt gagagtggag tgcttggagg atgggttagc gaggatgagt ggcgctgggt
    50641 ccatgcgcga attggtactg tgcataccgc tcaagggcgt gaagccgagg cggtcatttt
    50701 tgtgctggga gctccgcatc cgacacaaac tggcgctcgt ggttgggctg gtggtcgacc
    50761 gaatttgctc aacgtagcag ttactcgcgc gaaagaggct gtgtacgtca ttggcaaccg
    50821 acagctttgg cgtgaagccg gtttattcag agaactcgat gggaaacttc cgtgaagtaa
    50881 attaacgtaa gcgcggatgc tcggtatgaa tctgcccctg atctgcttga gttctgggag
    50941 tcgatgctaa tcaatagcca caaggcttga tccgcttccc ctatgggcgg ttcgccgcta
    51001 caatgtggct cagtcagttg atgttggggg tggggcagac atgaagaata cacgggatga
    51061 agtgctgaaa gtactggaag cgaatcgcca cgctctccat gcctacggtg tccgaagtct
    51121 gggtttgttc gggtccgttg cccgtgggga cgccacgcca gcaagcgatc ttgatttcgt
    51181 cgttcagttc gacaagaaat ccttcgacgg atatatgaac gtgaaggcct ttctggagag
    51241 tcttttcggg tgtcgcgtag atctggtgct tgagagcaca attaagcctc gattgcgaag
    51301 aacgatctta ggcgaggcag tgtatgccgc gggattataa agtatacgcg gatgacatca
    51361 ttggggcgat cgagaagatt aagcgattta cggtggggct tgatcgggaa acgttttctc
    51421 aggacgagaa gacgttcgat gcggtgattc gtaatctcga agtaatcggt gaggcggtca
    51481 agaagattcc cgaagaagtc cgaacgcgct acccgttagt ggaatggaag aaaattgcgg
    51541 gggtcagaga cattcttgtt catgaatact tcgggatcga tgtcgagatt gtctgggata
    51601 ttctccagca gaagctgtct ccactggaaa gtcagatgag acagattttg gcagaccact
    51661 gaaattcaac tctctgtaca cccgctctca gccttcgctg agagcggtgc gctagtggcg
    51721 atcaggccgc cgcgcgcgtg tcggtcttgg cggcactgtc cttccactga cccacttcga
    51781 ggctgtccag ccagtccagc agcttgccga ccatctcgcc ttcgaagatc gagccgtgct
    51841 gcgggcagat catgcggggc ttgatggcgc ggacccgttg tgtccacccg cgcagggcga
    51901 cggtggacgg catccagcgc aggtgaaacc ctcgcatgta ttggatgtgc tgctcgaaat
    51961 cggtgaccac caggctggca tgatggtcgg ggaccagggc gccgccgatg tcccccgaaa
    52021 agagaatgcc ggcggtcgga tcgtagagag aaaaattgcc ggaggagtga cagtagtgag
    52081 ccggaacggc ttcaacctca gggccttcgt cgccgatcct gatgcgcatc ccctggtctg
    52141 gaatcgggtt caacgtgatc acctcgccgg tgctgaaatg gctgacgaag cctgtccaga
    52201 gccaggagca ataggttttg atcgcggggt tcaggtcgag ccacatggcc agcgacgagc
    52261 agatgtccgg gtcctggtgg ctggcaaaga tcacgcggat gtcttgcatg cgcacatact
    52321 tcgcgagttc cgccaggacc tgtgggaaga tttgaatgcc gcccgggtcc agcaacatgg
    52381 catggccgcg attgatgatg acgtactcat tcgtgtcgat gacgtgactg tccttttcgg
    52441 gatcgcggcc gatgacgatc cactggtgtc cgtcctggtt gtacaagact ttactttgca
    52501 tacgtgtgcc ctttcagcag cagttctaag agctcctgcc cgtgctcgat ggcctctttc
    52561 atggcgcggg ttttggcatc gagatcatcc acgacatggg tgatctccgt tgccaggttc
    52621 ttgaagttgg cttcggcctc ggccaatccg gccgcttccg aggcgatgca gaaggccatg
    52681 tagcgcgcgg tgagaccgat cgttttcaga ccgaccaagg cctgctgggt ttcccccagg
    52741 cagcgcagcg cctctttcag ccgctcatgc agttcggcca ggttggcctg gcacaaatgg
    52801 acgatatagg cgcgattgtc acgttcgagt gcctcggcgt ccgaaaacgc gggatggtgc
    52861 atcaattgag ccacatctgc cgccgtggtg aagcggaggt gagacaaagt ctccagatgt
    52921 ttcgaggttg atgtcggttc cgcatgcgcc atcgccgaga ggaggctcgc aatcaattgg
    52981 tggtagcgcc agacaatatt cgaactgctg gcggtgacct tggccagccc cgcacagaga
    53041 tgctccaggg cctctacctc aggcctgatt tccttggggg tgttgttcag gatttcgacc
    53101 agtgccagca tgggtcggcc ttttccacca gggattttgg ccgcttggac cacgccgtta
    53161 ttcgcaagaa tttgcatgga cagcgagagc tgctggagag agcggccgac gttgaggata
    53221 tcgaccgtaa tcccgaatag ttgattgatg ttccgtttaa agaggctcag cacgtcatgc
    53281 atagcggccg tcgaaatcgt gtgtagttgt gggtcggggt gaaggctccc gcagttccct
    53341 atcggtacat ctctggaaaa ctgaaatccc ttccggtctg tgctgtaagg gcgcgtcgat
    53401 attttgacgg tcttgccggc atcggcgcca acgtcacggt ggttgaatgc cgagactcgc
    53461 gcgacggcgt ggtataatcc cgcgcttatg agcaattcca tcatcatcaa aggggcgcgt
    53521 gagcacaatc tgaagaatct cgatgtcgag attccgcggg ataagctagt tgtcatgacc
    53581 ggcttgagcg ggtcggggaa gtcctccctg gcgttcgaca cgatctatgc ggaaggccag
    53641 cggcggtacg tcgagtccct ctccgcgtac gcccggcagt ttctcgaaca gatgggcaag
    53701 cccgacgtgg actccatcga gggcctctcg ccggccattt ccatcgaaca gaagagcacc
    53761 agtcacaatc cccgttcaac cgtcgggacc gtcactgaaa tctacgacta cctgcggctc
    53821 ctgtatgccc gtgtcggcca tccctactgt tttcaatgcg gccaggaaat cacggcacag
    53881 accgtccagc agatggtgga tgccatctgt gaactaccgg aagggacgaa attccagatt
    53941 ctctcgccta tcgtgcgcgg gcggaagggt gaataccgga gagaattgct ggagatgcgg
    54001 aaggccggct atgtgcgggc ccggatcaac ggggagatcg tcgatctcgg tgaggacatc
    54061 acgctcgata agcagaagaa acacaatata gagatcgtgg tggatcgact ggtcatgaag
    54121 ccgggtgacg cgttgatgcg gcgggtcgct gactccgtcg aaacctccat gaaactggcc
    54181 gggggcttgg tcggcgttct gtccgaaacc ggcaaggtgc atctctatag tgaaaagctg
    54241 gcctgtatta aatgtggagt cagttacccg gagatcacgc cgcgcatctt ctcgttcaat
    54301 agcccgcatg gagcctgtcc ggcctgcgac ggaatcgggt atgccatgac gccgggcgca
    54361 tcggaagaag aggactttac gctgctggag cggtgcgaga cctgcgacgg ggcgcggctc
    54421 aagccggaaa gtctggcggt gaagatcgct aagaaatcga ttgccgaagt tacgatgtta
    54481 tcggtgcggg ccgcggcgga ttttttcacg tcactcaaat ttaccgaacg cgaactcgtc
    54541 atcgcccatc gcatcctgaa ggagattcgc gagcgtctgg gatttctggt caacgtcggc
    54601 ttggattacc tgacgctgga ccgcccggcg gccacgcttt caggcggcga gggtcagcgc
    54661 attcgtctcg ccacccaaat cgggtccggc ctggtcggcg tgttgtacat cctggacgaa
    54721 ccgtcgatcg gtctccatca gcgggacaat cgacgtctgc tccagaccct gcttcgcctc
    54781 cgtgatctcg gcaacaccgt catcgtggtc gaacacgatg cggagaccat ggaagcggcc
    54841 gactacattc tcgacctggg gccgggcgcc ggtacgcatg gcggccgaat cattgcccag
    54901 ggtacgccca agcaggtgat ggcgaatccc gactcgctca ccggaatgta cctgcgcggc
    54961 gagcagatgg tctcgctccc gccgcgtgcc cggaagccca agggctttgt gaccgtcgtg
    55021 ggggcgaaga agcacaatct caagggcgtg accgtgaaca tcccgttggg cctgttgacc
    55081 tgtgtgaccg gcgtgtcggg ttcaggcaag agcaccctcg tgctcgaagt gctgttccat
    55141 tcgctctccc agctgctcta tcacaagaag ccgaagatcg acggttgtaa ggaactcaaa
    55201 ggtgtcgatg cgctagataa aatcatcgac atcgaccaat cgccgatcgg ccgcacgccg
    55261 cgatctaatc cggcgaccta caccggcctc ttcacgttca tccgggactt gttttcaaac
    55321 ctgcccgagt cgcgcgtgcg cggctacaag ccgggccgct acagcttcaa tgtgaagggc
    55381 gggcgttgcg aagcctgcca gggcgacggc ctcatcaaaa tcgagatgca tttcctgccc
    55441 gatgtctacg tcacttgcga agtctgcaag ggacagcggt acaaccgcga gaccctggag
    55501 attcaataca agggccggag catcgccgat atcctcaaca tgaccgtgga cgacgccttg
    55561 gagttcttcg agaacatccc ctacatcaag accaagttgc agacgctcca cgacgtgggg
    55621 ctgcattacg tcaagctcgg tcagtccgcc accacgttgt cgggcggtga agcgcaacgc
    55681 gtgaagctct cgcgtgaact ctccaaacgt cccaccggcc gcaccatgta tattctggat
    55741 gagcccacca ccggcctgca cttcgccgac atccagcgac tgctggacgt gctcgatcgt
    55801 ctggtcgaga ccggcaatac tgtcctggtg atcgaacata atctcgacat gatccgcaat
    55861 gcggattgga tcattgacct gggacctgaa ggcggcgacc ggggcggcga aatcgtagcc
    55921 gaaggcccgc cgaaagagat cgccaaggtg aagaggtcgt acacggggca ggtgttaaga
    55981 gatgcggggg tgtaagggcg gagcgttcgg agtcgtcatg atgcgatggg cggagacggc
    56041 tcatgtcccg ctaccggctg ccgtgtgcga ctgtgaggcg cgaccgaagg tcgcatgccg
    56101 tcgcacaacg gcacgtttat ccgcttcatc tcgccacgcc cgcgatgccg actcccgaaa
    56161 gctccatccc ttccaccccc gctaagaagg aagaggggag gggaggcagg gggcgcaatg
    56221 atattcagcc tcctacgggc gctgagctag aaacgtagtt gcatgtccac agcagaaacc
    56281 catcgagaga taatctccat catcggccaa gagtacttca cccctattgc ccatttaatt
    56341 gataaatggg tggatagccg ccccgtgcgt cgtgattccg ttggagctcc gttcttcgaa
    56401 ggtggttatg cggtttcggt tattattctg ttagtcgctg cagtcgagtc gttcgtagcc
    56461 agagatcgct atttcagcaa gaggcagccg tatgggaatc atgctgctgt cccagagtac
    56521 atgaaggaga tgtatcgata tcgtggatac aaacgtctct ctgaattgtt tgtagttcga
    56581 gatgcagtca tccacagcca tgtttgggcg ttgaagttcg ctattcgcaa atctggcagt
    56641 cgccggttcg tctccgctag tcgaataagc tggagtggaa acaaccggct caaatcacgg
    56701 ctaaacccga agacataccg caccaaactg ctacgcttta acaccattcc ttcacgcata
    56761 gacagaaccg atgtaatcaa ggcatttgga atcgttctgg cggctattcg tttcctgacc
    56821 acgagcggcg cttatcctgt gcccgtcttt ccctttcggg tacaacatca aggggaacgc
    56881 atgtctttcg aggaactgcc aaattgtttg gccaagctgt taccgcctag tacgggaaga
    56941 gagtgagcgc attactcaga atttctttcc ccgctactgt ttgtctacat tcaagaggag
    57001 gcccatgcac accatcctgc tactgaccat ctcgaacatc ttcatgacgt tcgcctggta
    57061 tgggcatctc aagtacaagg agtcgccgct gtggctggcg attctcgcga gttggggtat
    57121 cgccttcgtg gagtactgtt ttcaggtgcc ggcgaatagg atcggccact atgaattcac
    57181 tgcggcgcag ctcaagacga ttcaggaggt cattaccctg atcgtgttct gcgtcttctc
    57241 agtcctgtat ctgaaagaag atctgaaatg gaactacttg gtcggcttcg gaatgatgat
    57301 cggtgccgtg ttttttgtct ttaaggaatg gtgatcgcgg tcattcgaca gctctttcaa
    57361 cttcttccta ctgttagacg agccttccca gttcccccca atcctttggc gcctcgatca
    57421 aatttttcct cgtagtctcg gtcctggcat ctgacaggcg ccagcgttag ccgctttgct
    57481 agcgcctttc ttccctctct ccagggtaat gactagggtc tccccggatg gttatcggtt
    57541 acaacgtgag tagaaggctg gattgggaaa tgatctcacg ctgacagccc cgagaaggag
    57601 gcatgtatga agacagcaac atcaatgaaa aacctgggat tgggcctgtg tgttgtggca
    57661 gccggcttca tggtcggggg ctgtaacaca acgaaagcta cggtcgacac gaccgtcaat
    57721 ttcttctcca gcacgagccc caatgggctc ttttcagcgg acggcatggt gctgaaagag
    57781 cagaagatca atctctttgc aggggtggcg tacgagaacc tccgtcagga ggctgcggct
    57841 ggtgggggcc agtacgtgag tgccttggcc tcgctgtatg aaatcccgtc cggcaaacag
    57901 gaggattttg gcctggtgct gcaacgcaag cacgccgact tatttgcggc gggattggaa
    57961 gacgatcgca cagcgcatct gaagatggtc agcgctctga atcgtgagct ggtggcagcg
    58021 tcgctattac cataatctgc ggttggtcgg gaggaatgta tgggaacgat gggaaaagga
    58081 tttatcctct ggttactcgg agttccggca agcctgctgg tcctcttatg gtttctgggc
    58141 attctcaagt aaccaggcta tgcagaggag attggcttgc gggcggacag gttgtctgtg
    58201 cctgaggagt cagtgtgaga ctcctgacgg caggagcgcg ggaccgaatc cctcctgttt
    58261 tgtccgcccg aatggctgag gtcctggtct tctacttgaa ccaacggtcg tacatataca
    58321 gcaaatccag cgtcagtctg gcgccgaagg tcgggccgta gtctttctgg ttgatgagat
    58381 cgttccaggt ttcgaacgtg aaggccgagt agcgcaccgc gactgagacg aatcgttctt
    58441 ccaacgcatc cccccgatga tctacgaaca gacctgaatc gacggtcacc gccagttcga
    58501 tttcccatgg agtcgagtcg gcccagtgcc gatagttccc gaggccgaca gagccctgag
    58561 ccatgtagga ctgtggcgcg acctggtgaa acgcggcacc gctgaacgga cggccgtatc
    58621 gcgcgagggc tgaaacgcga aggtagtcgc ctagcagcgg taccggagcg aagagggcca
    58681 accggcgaaa tcctgcctgc acatagggtt cataatacaa cgacccaccg gcgccaccca
    58741 gaccggcaaa gaacgcatcg ttcgatccca agagactgaa ccagcgggtc agcgacccgc
    58801 tgagcatgaa gtcgttcgct tcgcgtttgt tcccgaccgg gacttcggtg agccctcgaa
    58861 acttgtgcac cacatcgttt tgaagaaacc ggctcggccc gtcgcgcgtg ggtccgccgc
    58921 ctgccgtgag attgagattc catcccggca attgggccag gggcttggtc cagctcaagg
    58981 tgaagaaatt caaccccatg gtttcccgca tatcgttgta gcgcctgcct tccccgtcga
    59041 actcggtaaa ccgatccagg agtgcggcgc tcaaggtcaa ggtagggtcg tgatcgggga
    59101 aggcgatggc gccccagtgg acgctatgcg ggggcgcttc ggccagggcg agtcccggga
    59161 ccgcgcagac ggcgatcacg agggataaga tccatcgacg atacatcggg gcgcatagtg
    59221 cctgtgtcga tttcatttcg caagtagtcg gtacgtaatc gcaccgcaag atcgcaccgg
    59281 tcgactgtgc ccgcgggctt gcagtcgcgg cctgtgctta ctagaatgcc cgtcgaattc
    59341 ggtcgatttg aattggtgct cttcatctcc attgcgggga attatgccgc cgaagaaaaa
    59401 acagaccacg accggtaccg gctcatcgac ggacgacgca gccgagttcg aaaagctcgg
    59461 cgtgttttat ctcgggcggc cttatgatct tgccgccaaa caggccaagc ccggctggct
    59521 gctctacgat tcgaaggatc tggtcactca cgcggtctgc gtcggtatga cgggaagcgg
    59581 caagaccggt ctctgtgtcg gtttactgga agaagcggcc atcgacggca tcccggccat
    59641 cgtcattgat ccgaagggcg atctcgccaa tcttctgctc acctttcctc agcttcgcgg
    59701 cgaagatttc gcgccgtgga ttaatgaaga cgacgcccgc aagaagggct tgtccgccgc
    59761 cgacttcgcc gcgcagcagg cgaccctgtg gcagaagggg cttggcgact ggggccagag
    59821 cggcgagcgt atcaagaagt tgcgtgatac ggcggacttc gcggtgtaca cacccggaag
    59881 caacgccggt cttccggtgt cgattctgaa atccttcgcc gctccatcgg cagaattgct
    59941 cgacgatgcc gaactcctcc gcgagcgcgt cggcacgacc gtgaccagcc tgttgggctt
    60001 gatcggcgtc gaggccgatc cgatcaagag ccgcgagcac attctgctat cgtcgattct
    60061 cgacagcgcc tggcgagcgg gacgggatct cgatctgccc gcgctcattc atcagatcca
    60121 aacgcctccc atgacgaagg tgggtgtgct cgatgtggag tcgttcttcc cctcgaagga
    60181 acgattcgcc ctggccatgc agatgaacaa cctgctggcc gcgccgggtt tcgccgcctg
    60241 gatggaaggg gaggcgttgg atgtcgggcg tatgctctac ggcccctcgg gcaaaccccg
    60301 catcgccatt ttttcgatcg cccatttgac cgatgccgag cgcatgttct ttgtctccct
    60361 gcttctgaac cagacgttgg ggtgggtccg cggccaatcc ggcaccacga gcctgcgggc
    60421 tattctctat atggatgaga ttttcggcta cttcccgccg gtcgccaatc cgccgtccaa
    60481 agctcccttg cttaccttgc tcaagcaggc gcgcgcgttc gggttgggtg tggtgctggc
    60541 cacgcaaaac ccggtcgatc ttgactacaa aggcctggcc aataccggta cctggttcat
    60601 cggtcggttg cagacggagc gcgacaaggc gcgcgtgatg gaagggttgg agggcgcggc
    60661 ggccagcagc ggcaagaaat tcgatcgccg acagatggag caaattcttg cggggcttgg
    60721 gaaccgagtg ttcctgctga ataatgtgca tgaagatgcg ccggaagttt tccagacccg
    60781 atggacgctg tcctatctgc gcgggccact cacgcggaca caaatcaaac aattgatgga
    60841 tccgcagaaa cgtgaaacgt caaacgtgaa acgtcacgcg tcacaagacg agtctttccg
    60901 atcgacgaat ggcgaatcac gaatgacgcc cacgcggccc atgttgccgc cggaggtgcc
    60961 gcaatacttt gtcccgctac ggggacgcca accggagggg cacgcactcg tctatcaacc
    61021 gatggtcttg ggggtggcac aggtacgggt ggtggacaac aaggctgcgg tcgatgtgac
    61081 cgaccccctc accctactga ctccgctgac cgagggtgcc gtgccggtgg aatgggatca
    61141 tgcgacgatc gtcgatttgg ccgtagcgga tctggagcgc gatcctgcag gcggggctca
    61201 gttcgccacg ctgccggctg ccgccggcaa ggccaagcag tttgacggtt ggaagaacga
    61261 cttgtcaggc tggttattcc ggacgcaaaa agttgaacta ttccgtagtc cgagcacgaa
    61321 ggcgctttcg atgcccgggg agccggagcg tgactttcgt gtgcggctgc aacaggcggg
    61381 acgggagcag cgcgatcaac agagtgaggt attgcgaaag aagtattcac ccaagatcgt
    61441 cgcattgcag gatcgtatcc ggcgcgcaga acaaatggtg gaacggcagc aggccgagtc
    61501 ccgcagcagt cagatccagg cggccatctc ggtcggcgcc actatcctgg gggcgtttct
    61561 cgggcggaaa gcaatcagcg ccggcaatat cggtaaggcg acgacggcga tccgcggcgc
    61621 cggcagggcg atgaaagagt cgaaagacgt gagtcaggcg gaagacaacg tggcggcgtt
    61681 gcagcaacag ttggcggcgc tcgaggccga gtttaaagcg gaagccgatg ccctggctgc
    61741 ggcgaccgat ccgctcacgg aaaaattgga aaccctgtcg ttgaaaccta gcaaatcgaa
    61801 catctcgatc aagctcgtgg ctctcgcctg ggtgccgcat tggcgggatg agtcagggac
    61861 ggagacacct gcctggcaat gagccaggtg tcagcatact caaagaagaa agagcctatg
    61921 gcgtatcgct tatagcgtat gatccgatat ggaaagtggg tgtctgtgct accaaccata
    61981 agccatgcgc catacgctct tttccccgac gagatacgtt tcgcgaatgc cgaacatccg
    62041 gttagctgag ttgacgggac atacgaaatc gcgtcggatc cgaccggtct cgcgtaaacc
    62101 cgagttgttc gtagaaacgg agccagccga cgttgcggat ggacgtgccg gcccaggccg
    62161 gattgtccgg ccccaaatcg acgatcagat tgtgcccgga gtacgccgcc gttaattcct
    62221 cgatggcatg tctgaacagg cggtgcccca ggcccaagcc tcgataggcc ggtcgtaccc
    62281 agccatgcag cgcgagccgg tgtccggcca ccgaaagacg ggtcgcggcc actacggcca
    62341 ggatttcccc tggcccggcc gaatcgtccg gtgagggcct ggctttcagt tgcatgtgcc
    62401 aagccgctcg ccgcgtgtgg tcgccggtcg gttcctccaa cggcaacggt ttgtggagcg
    62461 cctgcatgaa cgtctctgcg taatccggca cggaatccca ctcaatcgac aggtctctga
    62521 cgagcgatga gagatcactg gtcggcccca tcgcttccaa cacgaccact tcatcgatac
    62581 tgagattcaa gtgttgctcg gcgaagcgga cgaattgttg gctgtacatg ccgcagagcg
    62641 tcggccagaa gagacggaag gtctcagcgg aggtccagcg ccggaacagg ttcatccagc
    62701 cgcgattgag ggcgtgattg tggaactctt cgaggttcac ggccaggtag acctgttcca
    62761 tgagttggat ctgcagattg caggtgtgca gcgcggctcg cggattgacc gcgatggccc
    62821 cgggatcgat cccgaacacc gagcggagtt caggatagat ttccaggtcg tagcgaagca
    62881 ggtccggatc gtcgcgcagg aggcgttcga gatccttcag gtcgtcggtt tcgccgagga
    62941 aggattcacg catgcccggc ggggtggtca gccagtagga gcggagtgca tggcagagcg
    63001 aggagaagtc ctgctcgctt cgctgaatgg cggggcgcag cacctgttga gcgatatgtt
    63061 cgccgaggcg gcggtaggat tcgaactggg cttcgtcgaa gaactggtcc gaggtcggtt
    63121 cgtggggaaa cgaagggtga cgcgcggcgt agtcttggac gtccgacggc tcgttcccgg
    63181 tcagcgaggg tttgagatag atgagggtgc cggcggtggc gttggcgtcc actttgtcgt
    63241 agcgaatcat gccgatggcg tggtggcggt cgctggtttt ggcgtcggcg gcccgcttga
    63301 tctgctcgag attcagttcg atgtcgatcc ccagatcgat gcggattttc cggatggcgt
    63361 tgccgagatc ttcaaaggtg agctgcgggt cacacccggc gtccaccacg aggatgcagt
    63421 gacagcggcg caagaccatt tcgtacaagc cgagattttc gaaatgcccg ccgtcggaga
    63481 ggtagacgta gggatgacag gagtccgtga ggccgaaggc ttcggacagc aaggggccga
    63541 ccgagacgtt gggtgacgct ttgcgatagg tgtcggcgcc cgcacggccc gggtttccga
    63601 gccaccagcc caggcggatg ttgaagagcg cgaggagaaa cgtcacggct gaagatgagt
    63661 gatagcccat gttggggctc gcggcggcgc ctgagatcgc cagagcggtc ccgagagaaa
    63721 ggggttgggc gaggtaatgg ttgcatccgt actcctccga gcggcgatac cccagattcc
    63781 aactgccgct gtggagcgcg ctgatcgtaa acgattgggc tttccgttgt tgccaggcca
    63841 ggttgtcgcc cttgaccaga ttgagcgcga tgttgatgag atgcagcggg cgcgggctct
    63901 ggtcgtgcaa cttattcaga aaaagtgtgg tgagccaggt gatggcgtca cgacctgccg
    63961 gcgtctccgg ttgttgcaag gcggcgatct tggcgcggca ggccggtccg acacgcagct
    64021 gttcggcggc cttggccagc gccttgttcg tgagcgaatg agagagctcc tgcgtcagtt
    64081 gcgcgatcat ggcctgaagt tgggccgatg tcggctcctg gagcgcgtgg aacgcatcca
    64141 gctgcgcgcg ggtgtccgtt gaaaatactt ctctggctat ccggcgcgcc aggcgcacgt
    64201 cgtcgaatcg cacgtcgctc atggccgggt tatccagatt gtcgaatccg gtgaagggat
    64261 tgaagctccg ttcgcgttcg gtctgaatgc gcgaggctcc gagataggcg cggatgagcc
    64321 ggttgcgata catcgcatgc aacgaaaacc ggttgatatt gatcagcccg gacatgagca
    64381 ggcccaacaa ggtcacggcg acggtgaaca ggaagagcat ccagaggggg gtgttgtgga
    64441 ggacttgatc gtggccccag ggatccggcc agatccctaa tccggtctgc ataggccccg
    64501 taagatggtt gaggtgcatc tcgtagtgat gggcgaagag gtctaattgc caactggtgc
    64561 ccagtgacag tgcgatgagg acgcagagca tgaagaaggg cgccgccgct cgtaccgcga
    64621 agtcggtgat cacggtggta tgtgatcgtt cctcctgctg ttgcgcggtg gtgcgtgagc
    64681 cgaatccaag cacgagggtc agcaatccgg agagtccgcc gagggaagcg gcgatcttcg
    64741 gcgtccaggc gatgagaccg ggaccgaagg cgacgatggc ggagagcccc gaccagatga
    64801 ccatgacgat caacacccag gaaccggtgc gcccccacca ctcgcgatcc tgctcttcgg
    64861 tgaaccggct ggccaggccg atgaagacgg tcgccgtgag caaaaatatc gcgaggacgc
    64921 ccggaacggc gaaggtggca aagcactcgg caaatgtggc gacgaccagc tgatcgggag
    64981 tcttcatcag gatagatcgg agaaacaatc cgcccagcgc gccggtggcg gcgacggcgg
    65041 cggtttccca gaacagccag ggggagaacg ctttgaatcg tttcagcgcg aggacggaaa
    65101 agagccagct gcctgtgtgg aggaagacgc cgccgagtat ggcgtggggg aggctgagtt
    65161 gttccagcgt gccgcctccg ttgcgaaacc aggcccaggc tgtggtgagg aagagcaccg
    65221 atgtgatcaa aggaccgagg caggcgacca gaaaccagtg ttggcgttcc aatgtctgcc
    65281 accgggtatt gcgccggtac tcgcggagcg taggacgaca gaggtgcaga tagatgagcg
    65341 ccatgacggc cagcccgaga ccgatcatga acacggcttg gagaagccag acgggcaagg
    65401 tccgtccggg ggtattcagc tgggccaggg cgatggtcca gcgtggcacg agcaggggta
    65461 ccatcagcaa tggcagcagc accatccagt tcaggtgcag gttgcgcaag taaatgccga
    65521 agagcgtcca tgagtcggcc gagaggaatc ccaggtgcgg actcaggtaa ttgctatagt
    65581 tccgcagcca ttggaccggc ggcggcactt cggtggcgcc tgttttcggc gtgacccgca
    65641 gatcctcgat gacaccgtcc aatccctgcg gatggcgatg gatccaggcg gtgagccagc
    65701 tgccgatgta gcctccgccg gagacggtcg aaaggtagtc gaacttgttc agaagctgca
    65761 ggcgcgcgag agactgcaag acgccgagtc cgaaggtcgc gctgcgtatg cctccgccgg
    65821 agaagcagag tcccgcgcgt ttcgggtggc aggcgtggat gtgccggaag agcgcggccg
    65881 tgcgcaccgc atcgggggcc cccaccggat gatccttggg cagggggccg tgcagcagtt
    65941 cggtttcttc ctccagaatc gttgcgagtg tcagcgggtc gtccggcgaa gcgggagtag
    66001 tcatcgtgac tccaagggga acatggtgag ggtggcgttg tctcgacgga aagcgcccgt
    66061 gtcggcgcct gtcataccac gattggaaaa ggcaaaccac atgcccagta aggacggtag
    66121 aacagaacgc ggcccggcaa ccctgtcgat gcgttccgtg ccgtctgcgg atacggaaat
    66181 agacgcgaat gcgtagggca ggtatgttgt ggagattatc gaatcggata cagcgcgtat
    66241 ctcaagtgat acggcaccct ggcgtcgcta tggtcaatgc ttgcggtccc gctgcatcag
    66301 cttgtcggtg taggcgagga agatcgccga acccaggaac gtcatgtgaa taaagatctt
    66361 ccacttgatg tgttcggggt tctcgttggt cacgtccaca aaggccttta acagatgaat
    66421 gctggagatg ccgacgagcg aagcggccag tttgatcttg atggtgcctg gatccacatg
    66481 ggtgagccac tccgggcgat cggggtgggt ttccagatcc agtttgctga cgaaggtcgc
    66541 gtagccgccg atgatgacca tggtcagcag gttggcgacc atggtgacgt cgatcagcga
    66601 cagcacaccc agcataaaca gcgtttcggt ttcctcgcgg aaatggacga tcatgtgcca
    66661 aagttccgtc aggaacttgg cggcatagag cagttcggcg atgatgagcc cgccatagag
    66721 cggcgcctga atccagcggc tgccgaagat caggatttcg aagagctgct cgacacgatg
    66781 ggctgctgta gacggtggag acggggtatg gggtgcagac ggatgactca cgccggtatg
    66841 gaccctcctt gttcagggcg tatcctagtc ggcgcgtgga gttctgtcaa tcgtgaggtc
    66901 gcgaggcgtg atgccctggc cgactagccc cccgcaggcg cgtcgttcgc gacagagggt
    66961 ttgaggtctt cgggtctgac atcgggcagc cagtgcgcaa ccttcgcctg gaggtcggcg
    67021 gcgatgatcg gtttgctgat gaagtcgtcc ataccggcct tcaggcaggc ttcgcggtcg
    67081 ccggccatgg cattggcggt catggcaatg atgggaagac gggtacccgg ctgttcctgc
    67141 gaacgaatga gcctggtggt ttcgaatccg tcgagttccg gcatctggca gtccatgaac
    67201 accaacgggt acgggtgccg gcagatggcg gctaatgcct ccattccggt tcccgcgagg
    67261 tccacgcgac agcccagctt ctccagcatc ttcacggcca cgcgttggtt gatggggttg
    67321 tcatccacga ccagcacacg ctggaccgta tgggccttga cctcggtcag ggtgtgccgc
    67381 gtgatcaatt gcgccttctc ctcacggcct tcccggccaa gcgtcaggcg catacaatcg
    67441 gccagatggc gctcacgaat cggtttggtc aggtacgcgg cggcgcccgc ttctttcgcg
    67501 agcatgctgt ccccgcgctg gcccgaggcc gtgagcagaa tgacgtgggt tttcgcgatg
    67561 gcaggatctt ccttgatgag ccggcagagt tccagtccgc tcatctccgg catgtggaca
    67621 tcgacgatgg cgatgtcgaa gggggtgcct tcctgcgctg cctgccgtaa gaggttgagt
    67681 gctgacggac catcgacggc actctcatgg tgcatgttcc aggctgagac atggtactgg
    67741 agcagactcc gattcgtggc attatcgtcg acgaggcaga cgcgaaggcc gctgagattc
    67801 ttcacaggca gcggaggcgt gatgggggcg gtgccttctt ccggggtaga aaacttgacc
    67861 gtgaacgaaa tgcgggttcc caggcccggc atcgactgca gcccgatttc cccgtccatc
    67921 agttctacca agcgtttgca gatcgaaagg ccgagccccg tgccgccgta tttacgagcg
    67981 gtcgagctgt cggcttgaga aaaagcttga aacatcttgg cttgggcttc ctgcgtcagg
    68041 ccgatcccgg tatcgatgac ctcgaaccgc agggtgacgg agcctgaact ctcatccgtt
    68101 tgcatcacct gaatcagcac ttcgcccttc tcggtgaact tgatggcatt gccgaccaga
    68161 ttggtcagaa tttgtctgat ccgtccggga tcgccgatca cggttcgcgg ggtctgcgca
    68221 tcgatgagcc cgaccagttc caactggcgt ccctgggcgg tcggtgccag gatgtccagg
    68281 gtgtcttcga tggtcagccg caggtcgaag gggatgtgtt caatggtgaa tttgccggcc
    68341 tcgattttgg aatagtccag aatatcgttg atgatgcgga ggagcgattc gccggaatgt
    68401 ttcagcgttt ccagatagcc cttctgctca gcggtcagct cggtgtcgag caagagggtc
    68461 gtcatgccga tcaccccgtt catcggggta cggatttcgt ggctcatggt cgccaggaag
    68521 tcggtcttcg cctggccggc ccgcacggct tggtcgcggg cttccgagat ctcccagttc
    68581 ttccgttcga ggtcctgggc cgctttcgac agcgcgcttt ctgtgcgttt tcgcatggtg
    68641 atgtcgacga tggatgccag cgccagtttt ccgttcggcg tatcgatcgg gttgagtccg
    68701 agctcgacgg gaaactcctg cccgttctta tgcaggccga agagttcccg gccggctccc
    68761 atggcgcggg acgacggggc ggagaagtat ccgatgcgat gcgccggatg gtcgggacaa
    68821 aaccgttccg gaatcagcat ttcgaccggc ttgccgagca tctcctctcg gctgtagccg
    68881 aacatggttt cgacctgttt gttgacgagc gtgatgttgc cgaggggatc gatcatcaac
    68941 atgccgttcg gcgcggattc caccacttga cggaactggg cctcggcctg tttgcgcgag
    69001 gagatgtcgc gtacgatacc ggtaaacttt ctgacgccgt cgacgagcat ctcactcacg
    69061 gccaactcaa tgggaaatga cacgccgtca tgtcgaagtc ccagcacttc acggccgatg
    69121 ccgatgacct ttttctggcc cgactgcaaa taattggtca ggtaccggtc gtgttccgtg
    69181 tggtaggggt cgggcatgag gagtttcacg ttctgcccca gcagctggtc cggggtgtac
    69241 ccgaacatgc gagtgacagc gggattgagc gattcgatga tgcctcgttc gttgatgacg
    69301 acgatgccgt ccaccgccgt gtcgacgatc gcgcgcaacc gggcctcgct ctgatgcatg
    69361 gcggcttccg cctgtttgag gtccgacacg ttgatgtgca tgaccacagc gccgtagctc
    69421 gggctgccct gcttcggggt gacgatgacc cggaaccagc gtcggagtgt gggacttgag
    69481 ctctcgtatt catagtcgaa cgcacggatg gtgccgtcca ggatgtgccg gattccttgg
    69541 gccacgtgct gggcgtcggg ctgaggtccc gccgcatcgc agatgtggag atagttgcgg
    69601 ccgacgccgt agccctcgcc ggtaaagccg ttcgcggctg cgaaccgttc ccaggactca
    69661 ttgaccgcta gaattgtccc cttcgcgtcg agcacggcga tattggccgg caggacgttg
    69721 aggatgctgg gatcgagggc attgtcctgc ctccgtctga tggggaggtg atggacggcc
    69781 atacagcaga ctccttccct acccggtggt gccggttgca gcgccggcgg ttccgagcca
    69841 ggtgtcgagt accgcctgaa gctctttggg gcggaccggt ttggcgatgt aatcgtccat
    69901 tccggcggcc aggcagcgtt cgcggtcgcc ttgcatggcg ttggccgtca tggcgacgat
    69961 cggggtatgg gccgaccggc cttccatttt gcggatcagt gcggtcgctt caaacccgtc
    70021 cacttccggc atctggcagt ccataaaaat cagcggataa cgggcacgct catgggcggc
    70081 cacggcttcc tgtccgtttc ccgcgacatc gacccggtag ccgagtttct ccagcatctt
    70141 gcaggcgact ttctggttca cgggattatc ctccgccagg agcaggcgag gcttcggtcc
    70201 tgcctgggat tcgctcaggg tgtgtcgtgt gatcaacggc ggcggttcgg cgttcgacgg
    70261 cgtcccggcc gcggtttccg gggacagcag gagacacagg cattcgagca attgtgtctg
    70321 acggagcggt ttcgtgagat aggcatcgat gccgatggat cgcgcagtct ggccgtctcc
    70381 ccgccggccg accgtggtga ggatgaccag gtgtacgccg gcggtggcgg ggtcttgttt
    70441 cagcaaggcg gccgtttcga atccgtccat gtcggagaga tgcaattcga tgagggccag
    70501 gtcgaaggga ttctgagcgg cggccgccgt gcgggctaac tccacggcct ccgggccgct
    70561 ctgtgccacc gtgcaagtca ttccatgcga ggtgagctcc tggcgcatgg cttgcctgac
    70621 cgtgtcgcaa tggtccacca ggagaatacg ccggccatgc agccggctcc aggaaaaggg
    70681 cgacggggaa gcggcgagtg tcgtctccgg aaattgcacg gtgaaccaga acgtgctgcc
    70741 ctgtccggga tggctttcaa ccccgatccg gccatgcatc tggctgacga ggcgttggca
    70801 aatagcgagc cccagtccgg tgccaccgaa tcgtctggtg gtggagctgt cggcttgtac
    70861 gaaggcgtta aacagtcgtc cctgcgccgc ttctggaata ccaatcccgg tatctttgat
    70921 ggtaaagcgc aaaaggccca cgccgtcttg agtctcacgc gtcacgtgga ggaacacttc
    70981 gcctgcggag gtaaacttga tggcattgcc gaccagattc accaggattt gccggagacg
    71041 gccgggatcg cctacgacgg cggtcggcac ggcggcatcg actaacccga tcaactccag
    71101 gcctttgctc tgcgcctgtt ccgcgagcag ctctaccgta tcctccaccg tcatccggag
    71161 gtcgaacggg agttcctcga aatgaagttt tcccgcctcg attttggaga aatcgagaat
    71221 gtcgttgagg agagtcagca agctgtcgct cgaacggcgg accgagtcgg caaattcaag
    71281 ttgttctttg gtgagggcgg tatcgagcaa aagacccgtc atgccgatga tggcattcat
    71341 cggggtgcgg atttcatgac tcatggtggc gaggaagtcc gccttggcct gggcggctcg
    71401 aagggcttca tcccgcgctt gcgcaagggc atgttctgcg gcttttcggc tggtgatgtc
    71461 ttttccaatc gccaggaatc cggtcacgac tccgtcactg tcccggagtg cggtgatagt
    71521 caagaggaca gtcagccggc tgccgtcttt tcgctggtac gtccattccc gttcatcgaa
    71581 cccgcctcgt ttggcatttt cgatcaaggc ggcaacacca tgaatcgggt gctcgtacaa
    71641 gtcgctgagt tccctggcat ggcggtcgat ttcctcgggc acgtgcagca aggtgagcga
    71701 ctgttgcccg atcatctcgc cggcggaata acccagcaac tcttctgcgc cggtgttgaa
    71761 ggtcgtgatc gtcccctcgg tattggtggc gacgatggac acattggttg cgctggaaag
    71821 gatggtttgc agttgcaaat tggtcgcgag cagggcccgt tccaggtctt tgcgagtctg
    71881 gatgtcggtg gcaatgacct ggatggccgg tttcccctcg aacataatcg gggccgccgc
    71941 cacttccaca tcgacgatgg agccggcgag accgacaaac cgctcctcgg tgggcgggag
    72001 cggttgattc gtgtcgcgta ttttcgcgaa tctctcccgt accgtggggt gcgattcggg
    72061 atggatgatc tcgaggacgg tctttccgaa taactgcgac ggggcgatcg cgcccagtaa
    72121 cttcgcgcag gcctgattgg caaatacgat tttgtcttca caattcacga agatggcatg
    72181 gggcgacaat tccacgagcg cgcgatgccg cagctcactg gcacgaatct gtacctccat
    72241 ttgcttggct gcagtgatgt ctacggcgat gcccgcaatg cgataggtct ccccctggac
    72301 gttcttgatc gcgaagctac gatcccggat ccaacggacc tgttcgcttg gtgtgacgat
    72361 ccgatattcc tcgtcatagg ggagatgggc ctgacaggca acggcgatgg cgacgcggtc
    72421 acggtcttcc ggatggatgt gatcgagcca gattgtcgga ttcgcataga gtagatctct
    72481 ggggagttcc cagatggttt caaaggcggg gctgacatag aggacctcgc tctta
//
LOCUS       DAORRQ010000017        72132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_19_length_72132_cov_2089.326082, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000017 DAORRQ010000000
VERSION     DAORRQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 72132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19_length_72132_cov_2089.326082"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1556)
                     /gene="metG"
                     /locus_tag="PK224_07385"
     CDS             complement(27..1556)
                     /gene="metG"
                     /locus_tag="PK224_07385"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRB15595.1"
                     /translation="MNKRDTFYITTPIYYVNDVPHIGHAYTTVAADVLARYWRLRGRD
                     VFFLTGLDEHGQKVQQAAAKAGIDPQAHCDRLAPQFTNLWQRLNISNDAFIRTTDKQH
                     QDVVQRYLQQLYDKQLIYKADYTGWYCTYDERFWTEKDVVGGLCPDCKRPIEQLSEHN
                     YFFKMGQYQERLIDHIRTHDDFIRPVSRRNEVLGFLQTQKLGDLSISRPKSRLSWGIE
                     LPFDKDYVTYVWFDALVNYVSALEYKSGLERFWPANTHLVGKDILTTHAVYWSTMLMA
                     LERPLPHSIFAHGWWTVDGEKMSKSRGNVVDPNKMIDQFRADAFRYFLLREVPFGQDG
                     DFSRNTMVTTINSALANGIGNLLSRTLTMIERSCAGTIPDRGPAVLPELEARIEDLAR
                     QLPGRIEAGYNALLFRDVLLMIEELNTACDEYIDKSAPWKLAKQADTQPQLQTVLNTS
                     ARALRLLAILLYPFMPQAAQQMIRQLGLPLDLSKPISDTHSNWDTPLAGSKTGEKITF
                     E"
     gene            complement(1602..2633)
                     /gene="holB"
                     /locus_tag="PK224_07390"
     CDS             complement(1602..2633)
                     /gene="holB"
                     /locus_tag="PK224_07390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRB15596.1"
                     /translation="MPFADIIGHDSAKTLLRSAILQNRLAHAYLFQGDDRIGKRLLAL
                     RLAQALLCETVPDDQTPDACGACRACRQVDARTHPDFMVIEPDQELANPQIKIELIRD
                     IEHQMIYRPLIGDRKICLIDDADRMTIGAANALLKTLEEPPDHSLFILVSSRPYALPA
                     TIRSRCQALRLTAPAQTQVEAAVILKRELPPADAHFLAVLSDGQLGRALECDLAQARN
                     SQKEFAAIFSSKGLQSYATVLAAAEALSKEERGPEAFDWLLRWLRDVLLVAVGAGSDH
                     VLNLDQKAGMQALAERIDIDELLDLINDLEKLERQAHRNLNVQMALETILLRVRHLLT
                     PQDTADRPR"
     gene            complement(2620..3348)
                     /gene="tmk"
                     /locus_tag="PK224_07395"
     CDS             complement(2620..3348)
                     /gene="tmk"
                     /locus_tag="PK224_07395"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRB15597.1"
                     /translation="MMTPRSRPPRGLFITLEGTEGCGKSTQAKLLGEHLRNQGYDTVE
                     TREPGGTPLAEKIRSLLLDRADEPVAPETEAFLVFAARRQHVAQVIEPALARGSIVLC
                     DRFIDSTLAYQGYARGLDIATLERLNRLATHALMPDLTLVFDLPVSTGLARRRSAAEL
                     NRLDRESLRFHRSVRTGFLDLAKRHPARIKVLSARASKEAVARAVARVVTPMLGHIRR
                     GVDHNRPAASRPSPQKSQARHAIR"
     gene            3480..4154
                     /locus_tag="PK224_07400"
     CDS             3480..4154
                     /locus_tag="PK224_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRB15598.1"
                     /translation="MLKASLKRYFLTGLLIMIPFWGTILILKTLFVSLDGILGDATAQ
                     LVTPGYYVPGLGIVALILLIFVTGLFAANFIGRHVVRQWEGLLNRVPVVRGIYSTIKS
                     MMDILSFAERESYRRVVLIQFPKNGHYCFAFVTGVTKGEMQQLSPDPLVHVYVPTSPN
                     PTSGYFLLVPEREVIAVDITVEEAMKLIVSGGLYTPTPPSGGTSQTSGKTWAPIKQPD
                     AGVQVG"
     gene            4206..5825
                     /gene="glmU"
                     /locus_tag="PK224_07405"
     CDS             4206..5825
                     /gene="glmU"
                     /locus_tag="PK224_07405"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRB15599.1"
                     /translation="MTLSSQDKAVPSSIPGLGVIIMAAGLGKRMKSALAKVLHPVAGR
                     PMVMYVLDIACGLAEQGVAVVVGHQGTEVRKVVEAVGGQVAVAEQTKQLGTGHAVLQA
                     RPVFSGGAHRRPSRYLILNGDTPLLTEATVRELLAMHDAQGAAVTLLTAVLDDASGYG
                     RVIRRRRDEWLQGAADNAVQSIVEDKDASEAERAVREINVGTYVVDGEFLFPALDKLD
                     PRNAQGEYYLTDIVQMAVQQGRTVSALRLRAIDEGLGINSRVQLAEAEQVIRQRIREQ
                     WLEAGVTMRDPASTWIDAEVTIGRDTVLYPNVTLEGRTVIGESVVVHSGTRITDCAIG
                     DRVEILDHCILRESQVEEECHLGPFVHLRPGVIARRKAKVGNFVEMKKTELGEGSKAN
                     HLSYLGDATIGSGVNIGAGTITCNYDGYKKFHTVVGDGVFIGSDVQLVAPVTVGQGSI
                     IAAGATVTQDVPKDALVIARVPQVTREGWAARRRALQSGQVPPPAPSVKRAIPVKAAK
                     AVKRAKTAKAATSATRAVKKKQPAVQRTKRR"
     gene            5835..7667
                     /gene="glmS"
                     /locus_tag="PK224_07410"
     CDS             5835..7667
                     /gene="glmS"
                     /locus_tag="PK224_07410"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRB15600.1"
                     /translation="MCGIVGYVGNQDAVPILLNGLSKLEYRGYDSAGVAIQRGEKIEI
                     RRSVGKLINLQKSLEQKAIGGMCGIGHTRWATHGKPSEQNAHPHRSESCVLVHNGIIE
                     NYVELKQRLVKDGYKFQSETDTEVVAHLIDTHMKKSKLHLADAVRATAKEIRGSYAIA
                     VISEREPGMLVAARSGCPLVIGRTADASFVGSDVMAMLSHTRDVTFLEEGDVVEVTAG
                     AVTFTDLDGRAVTRKKTKVTWDASAAEKSGYPHFMLKEIHEQPQTILDTIRGRYSYES
                     GEADLPDIGLTPKEFAEVGRIWIVACGTSWHAGLVGKYLLEEMVRTPVQVDIGSEFRY
                     RDPLIEKNDLFITISQSGETADTLAAAREAKQKGARVVSIVNVVGSTLARESDGVLYT
                     HCGPEIGVASTKAFTSQLAALYMLALHLGRVRGVLSVADGKAWLDRLVTLPALVKHVL
                     GREAEILAIAKRYYKKPDFLFLGRGINYPIALEGALKLKEISYIHAEGYAAGEMKHGP
                     IALIDKDMPVVVLAPRDRLYEKTVSNLMEVKARRAPVIAFVAEGERELGKIADAVFTI
                     PDVHPLLSPILFTIPLQLLAYHIAVLRGEDVDQPRNLAKSVTVE"
     gene            7811..8104
                     /gene="gatC"
                     /locus_tag="PK224_07415"
     CDS             7811..8104
                     /gene="gatC"
                     /locus_tag="PK224_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRB15601.1"
                     /translation="MEITKQEVEKVAKLARLALTEAETTAFSQQLNQIVAYVQKLKSL
                     STEGVEPTSTVPGQSNVFRPDQVQASLSTEQALSNAPDAEAQCFRVPKIIQES"
     gene            8130..8495
                     /locus_tag="PK224_07420"
     CDS             8130..8495
                     /locus_tag="PK224_07420"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRB15602.1"
                     /translation="MFRQMLRAKIHRATVTEACLEYEGSLTVDEDLLDAAGILPYEAI
                     VCSNLNNGERFMTYAMKGKRGRGEIVLNGPTARKAAVGDQIIIFCYEYYSDEEIKRHK
                     PKIIQVDGKNRITRKAVKR"
     gene            8495..9970
                     /gene="gatA"
                     /locus_tag="PK224_07425"
     CDS             8495..9970
                     /gene="gatA"
                     /locus_tag="PK224_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRB15603.1"
                     /translation="MSLTLLHKLTLSELQRKFTAGDVTATEIVRAYFLRVTQVEPKVN
                     AYLTQCKEAALAQAERLDQALKGWRKTTPMMAMPLAVKDNICTEGVRTTCASRMLDTF
                     VPPYDATVVAKLRAQNYLLLGKTNLDEFAMGSSTENSAFGASRNPWNIQTVPGGSSGG
                     SAVAVAADECVAALGSDTGGSIRQPAAFCGVVGLKPTYGRVSRYGLVAFASSLDQIGP
                     ITKDVTDAAILLGAIAGHDPRDSTSANVPVPDYLKALKRKDLKRLKVGVPAEYFADGL
                     DPEVDQAVRAAIEGLRELGADIREIKLPTTDAAVATYYVIATAEASSNLARYDGVKFG
                     LRAAESKDLLDMYLKTRAEGFGPEVKRRIMLGTYVLSAGYYDAYYGKAQAVRTLIRQE
                     FEAAFQTVDLIVTPVTPTTAFKFGEKAQDPLQMYLSDIYTISANLAGLPAIALPCGFS
                     KAGLPIGFQLIGRPFEEETLLRGAHAYEQATNWRAKRPAIR"
     gene            9989..10390
                     /locus_tag="PK224_07430"
     CDS             9989..10390
                     /locus_tag="PK224_07430"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HRB15604.1"
                     /translation="MIVDVAAILVAIAFAVLVGYLVPLLIQIRKMVAESEQLVTKLNV
                     ELPTLITELRAMSQNLNDVTEQARGGVEHAAVLLHAVGEIGESVNQIHSVVRGSGGSL
                     LANVASVVAGFRAAKQVVTERFKEGGHHNGG"
     gene            10380..10700
                     /locus_tag="PK224_07435"
     CDS             10380..10700
                     /locus_tag="PK224_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRB15605.1"
                     /translation="MADNQGPSSAAVLLGFLSGAALGAVTAILLAPRTGQESREMLRG
                     YARRAEDGLRDLVGEAGERFEGAVEEGRDFIESKKTVLRDAFDAGREAMRREREHFTK
                     GEQS"
     gene            10703..12130
                     /gene="gatB"
                     /locus_tag="PK224_07440"
     CDS             10703..12130
                     /gene="gatB"
                     /locus_tag="PK224_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRB15606.1"
                     /translation="MAYEVVIGVEVHAQLRTQSKLFCACGTTFGLTANSQTCPVCLGL
                     PGTLPVINEKAVEMAVRAGLAMNGTIGVQNRFARKNYFYPDLPKGYQISQYEAPICEH
                     GWIEIAAGGSRKRVRIRRAHLEEDAGKNLHEVDSGMSLVDLNRSGTPLLEIVTEPDLS
                     SSEEVVAYLKALRDLLMYLDVCDGNMEEGSFRCEPNLSLRPVGQTMFGTKVELKNINS
                     FKFVKDAVDFEIKRQTKVLNEGGKIYQETRLWNHERGETAVMRSKEEAHDYRYFPDPD
                     LVPMEISSEWIEQLREGLPELASTKQQRFITEYGVPEYDAGILTSSKALAMYFDACVK
                     LYPQPKTVSNWVMGELLRELNNSGIEAAASPVTPERLVELLTLVDQGVISLKVAREIF
                     PDVYASGKAPARIVQEKGLTQVSDEGALATMIDEVLKKNPAQVGQFKEGKQQVLGFLV
                     GQVMKASGGKANPGKVNELLKKALA"
     gene            12243..13529
                     /gene="eno"
                     /locus_tag="PK224_07445"
     CDS             12243..13529
                     /gene="eno"
                     /locus_tag="PK224_07445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRB15607.1"
                     /translation="MSGIRNVKARQIIDSRGNPTVEVEVLLESGAHGRAAVPSGASTG
                     EKEAIELRDGDKKRWMGKGVSKAVANVSKTIAPRLMGMEALDQAAVDHEMIALDGTKT
                     KGKLGANAILGVSLAVAKAAANETGQPLYRYLGGTNARVLPVPLMNIINGGAHADNRL
                     DLQEFMIMPVGAPRFSDALRMATEVFHTLKSLLKKKGLNTAVGDEGGFAPDLQSNEEA
                     LALIMEAIEAAGYRPGQDIALALDCAASELYEKGRYRLEAEKNPERSSEEMVAYYGKL
                     LDRYPILSVEDGLSELDWKGWKILTEKLGKRVQLVGDDIFVTNVEIFAKGIAEGIGNS
                     ILIKLNQIGTLTETLDAIELAKRSGYTAIISHRSGETEDTTIADVAVATNSGLIKTGS
                     LSRTDRVAKYNQLLRIEDELGAAAVYRGRAAVPSRA"
     gene            13535..13897
                     /locus_tag="PK224_07450"
     CDS             13535..13897
                     /locus_tag="PK224_07450"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRB15608.1"
                     /translation="MMIKPNRGRDWLDWQRKLCSAGKWVGLGALFLMMGTLLFGEMGI
                     SRYLHLRDHAEQLDQELAELQRLNGELRTDLDRVQYDPTRIEELARERLGYVRKGETV
                     YQLAPIGEKERFPTVRTP"
     gene            13894..14778
                     /gene="era"
                     /locus_tag="PK224_07455"
     CDS             13894..14778
                     /gene="era"
                     /locus_tag="PK224_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRB15609.1"
                     /translation="MKFGSVAIIGRSNVGKSTLLNRLLKEKIAIVSDKPQTTRTRILG
                     VAHVEGAQIVFLDTPGFHEPHHLLNRRMVRTTLDTFDDADVLYVVVEATAQPGPGDLA
                     VIEHVKQAVAKQARPVVLVINKVDLVNKSRLLPLMEQYLRIFPWTEIVPVSAETADNV
                     DRLLSVTVSLLAEGEATFGDDVVTDQSMRTLAAELIREKILQQTYEEVPHSVAVEIEE
                     FIETKKLTKIGAVVLVEKESQKGILIGKHGERLKLVGTAAREDMERLFGTKVFLKVWV
                     KVRESWREDEQTLAELGY"
     gene            14882..16219
                     /gene="mgtE"
                     /locus_tag="PK224_07460"
     CDS             14882..16219
                     /gene="mgtE"
                     /locus_tag="PK224_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRB15610.1"
                     /translation="MRLVSIQRLLRRGAITNLAKMLARMHPADIANVIDHLSSLKEKR
                     EIFELVRGDVKRGQVLSELDGESITLVLSDLLPSDAAWLLKDLGSDDIAYILSVIPND
                     RAKEILALMRTEDSTEIADLLKYPKGTAGGIMTTEFFALFEDATAQEAIRRLQQATDA
                     EMVFYIYVTDKEDRLVGVLSLRQLLTVPPATPLKNIMTRDLISVAVDMDQEEVSRQVA
                     SYNLLAIPVVEKDGKLVGIITVDDVVDVIREEATEDMLKMAGAIEEDAMFKSSSMAAA
                     RVRLPWLFTNLVGSLLSGMLLWMFRYTIQEVVAIVSFIPVIAAMGGNVGLQSSTLIIR
                     GLATGLVELTDVWKIFFREAKIAFLMGIACSLMLTVVGWLWHQVFLGMVVGVSLITAF
                     LVSTSMATVMPIVLKRMGVDPAVAAGPFVTTANDITGIAIYLTLATIFLEQIR"
     gene            16229..17032
                     /gene="recO"
                     /locus_tag="PK224_07465"
     CDS             16229..17032
                     /gene="recO"
                     /locus_tag="PK224_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRB15611.1"
                     /translation="MGLLVSLSSFTLGGEMPLVKTAAIVLHSRKWGDADRIVTFYTMR
                     IGKLRGVARGARRLKSRLGGMIEPFTLCQLDLFEKPGDSLYRISQVALDEPFAKFRDD
                     LTLMTAAGRMVNLVSAVMAEGDPDPRVFEMLESGLRTLLESRDAAWTTLLFQIRLLGM
                     TGFRPQTEQCATCGQVHRSPLPQFSPMAGGIVCERCASRQPFRCTALSRGSVAFLHQA
                     LHMQPALLNRLHAAGQVRTEVESAIESYVTVVAGRRLPPVDFLTSGSPA"
     gene            17096..17452
                     /locus_tag="PK224_07470"
     CDS             17096..17452
                     /locus_tag="PK224_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRB15612.1"
                     /translation="MTETVLEPTDMEWVAKGVLSITWSDGHKALYPVRYLRQHCPCAA
                     CTDEWTGELRLKPDDVPMVMMLQDVQPVGRYAFQFTWSDGHDTGIYSYTFLRRMCQCD
                     VCQPVKPVEPRSRRLL"
     gene            17496..17957
                     /locus_tag="PK224_07475"
     CDS             17496..17957
                     /locus_tag="PK224_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15613.1"
                     /translation="MTQARIIAVGWWWLVAILSAGCSSLPSLDQQKQLVQQGDYRIHQ
                     LTPRAFVETWGEPTYTHQQFTHFFGMQDGQLIPQSRMALGESPQGWETGLAAGDALFL
                     AYADRGQYLVFLDEALVYHEAMTPEKVHAVGKTWKYESQFKTRLELSPAMK"
     gene            18018..19508
                     /gene="glgA"
                     /locus_tag="PK224_07480"
     CDS             18018..19508
                     /gene="glgA"
                     /locus_tag="PK224_07480"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRB15614.1"
                     /translation="MVSSEVVPFAKTGGLADVVGALATEFARLGHDVCVLLPAYRQVD
                     ALGYQVVDYARLAVPTAQGLVEARIQERTGPHSHVTGSGRLRVLTVRHDAFFSRSGLY
                     QEAGHDYPDNLERFAFFCRAVMELLVHFGEKDGWQADLLHVHDWQAALCAVYLRTLYQ
                     AKSALSNIRSVLTIHNLGYQGLFPAEHFSLTGLPAQLFTPAALEFYGKLNLLKGGLVF
                     ADRLTTVSPTYSEEIQTPEYGCGLEGVISGRKDVLQGIVNGIDTEIWNPAKDPHLPTQ
                     YSLSDMSGKARSKKGLQRELKLRADKGPLVGVIARLTGQKGIDLVIDIIPELMELDVQ
                     VVILGTGDVKYEQLVRELAERYPGRLAVRNVFDEGLAHRIEAGADMFLMPSRYEPCGL
                     SQLYSLRYGTVPIVRKTGGLADTVVNYTPSAFKGSRVTGFSFTDTSADSLLTCLLLAL
                     SIYRKKADWHRIVRAGMEQDLSWARSAEIYLRLFQELLEGKEPQRK"
     gene            complement(19534..20031)
                     /locus_tag="PK224_07485"
     CDS             complement(19534..20031)
                     /locus_tag="PK224_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15615.1"
                     /translation="MSDRRDIRVGILAILVAALSVVGCQSNPATTSVRYDNVRFMDVW
                     STYTHCLASNQSQSALLDSHKLREVSQAQALRSPLEKFLPTKLKSMVTQPASRLAVDV
                     HAMAAACSLHAGNLAVSVGEHDLARNEFRLVLENQTQSDYSYYASQARERLSYLDLTL
                     QAALR"
     gene            20842..22476
                     /gene="aepX"
                     /locus_tag="PK224_07490"
     CDS             20842..22476
                     /gene="aepX"
                     /locus_tag="PK224_07490"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HRB15616.1"
                     /translation="MNASQALTKTRQFRNLLLSEQLQFICEAHNGLSAKIVQEAGFPG
                     IWASGLSISAQFGVRDNNEASWTQVLEDLEFMSDATRIPILLDGDTGYGNFNNMQRLI
                     RKLEQRNIAAVCIEDKLFPKTNSFLKGDAQPLADMQEFCGKIKAGKDAQTDPDFCLIA
                     RVEAFICGWGLAEALRRAEAYHQAGADGILIHSALSVPDEILAFKREWGNRCPVVIVP
                     TKYYATPTDVFRQHGFSMVIWANHMLRAAVASMQKTARALKESEHLLSIEDKVAPVSE
                     IFRLQNAAELLEAEERYLPRGAEGTSAVVLAASRGEELGELTEDQPKTMVKVQGVPIL
                     SHIVDAYNAVGIKDILVVRGYKKEAVNLPNLTYIDNLEFAETGELASLSQALQSRKGR
                     FQPTIVSYGDVLFNKYIPQALCQEQDDCVIFVDSNWRDQSSYARLGGFAECSVPNSRK
                     AFNAKIHLKQLGKTLPEESIHGVWMGFLKLSSSAASQVNDLLLGMLADPSNRRAGIPQ
                     LLQELLKRDVPVRVLYTVGHWLDVNSLEDVVQAGNF"
     gene            22594..23721
                     /gene="aepY"
                     /locus_tag="PK224_07495"
     CDS             22594..23721
                     /gene="aepY"
                     /locus_tag="PK224_07495"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HRB15617.1"
                     /translation="MIDPREFIAHLQASGVEFFTGVPDSLLKEFCACLEHVPRAGHHI
                     ISANEGGAVALALGYHLATRKVPLVYLQNSGLGNIINPLLSLADPEVYSVPLLLVIGW
                     RGEPGVHDEPQHKKQGRVMLSMLEAMEIPHSILGPELVDPKAALADALAHVRKMSAPY
                     ALVIKKGTFQSFAAPKVEKRDFPLSREEAIQQVIESLGERDVLISTTGMPSREVYEYR
                     RKRGEGHQRDFLTVGGMGHASQIALGIALQKPGRPVYCLDGDGALLMHMGALAITGAL
                     KPRNFKHIIVNNGAHDSVGGQPTVAFDVDVPGIAHASGYESVFCAQTKQELQSCLAEL
                     QRSSGPSLLEVRVRCGARKDIGRPVTTPSQNKNAFMDFVEN"
     gene            complement(23860..24471)
                     /locus_tag="PK224_07500"
     CDS             complement(23860..24471)
                     /locus_tag="PK224_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB15618.1"
                     /translation="MAQNAVHSQEPADNLADQRAGAGIVVLSSSMQLLHMNRQAAELS
                     KLINMAENGGAPAKSAQGVLPAALTELCTEVLKALQVRTEAKDWEQFEVKRIAGNPNQ
                     PVLLRGFGLPDRGGIQYARLVVTLEELGRRQQLNTDQAKERFQLTNREQSVVENLAKG
                     WTNKEIANALKITEQTVKEHIKHIMRKTNSTTRTGVLVQVFRS"
     gene            25298..25999
                     /locus_tag="PK224_07505"
     CDS             25298..25999
                     /locus_tag="PK224_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRB15619.1"
                     /translation="MNGKALLSGIILVGNVIGVAHAGQFDQVPKPTLVAGSSPGASSS
                     GVRHGGEGAEKSSLTVTPDYIMGPEDVLEITVWKNADLSKQVQVRPDGRISLPLIGDV
                     SAVGRTAAQLTEEISARLKSYMENPTVSILVREVNSYQIYVLGEVNAPGKYPLKSKTT
                     LLQAITIAHGFTQVAARNKIVVFRFGKDGEGLNKIKASYDDIVLRDGSDQNIELKPGD
                     QIVVPSETMVVLPSR"
     gene            26052..26684
                     /locus_tag="PK224_07510"
     CDS             26052..26684
                     /locus_tag="PK224_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRB15620.1"
                     /translation="MGKLKTFGCRWFRGGVCIVFSLLLPLPGCWIGNEQIDFNVTYIP
                     PNEFLLGPEDVLVVNIWRNQELSREVIIRPDGKISMPLIGDVQAAGMTSNVLAKRIAD
                     GLAEFMSNPTVSVQVKEVNSYYVYVLGEVSKPGKVPLKSYATVLQGISLAGGFTTFAS
                     RNKIHVLRVVPNGQGQPKQVQIPVPYEDIIQGKNSEGNFFLKAGDVIVVP"
     gene            26608..28281
                     /locus_tag="PK224_07515"
     CDS             26608..28281
                     /locus_tag="PK224_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15621.1"
                     /translation="MKTLFKVKTRREISSSRRVMSLLCRDQCLKIGWLCYGVFALMSC
                     WTISDSAAQQIRVVPSISVIEQYDSNVFFTPKSLLAPGTKVDDFITVVTPQLNFMQSN
                     SLVKTNLSVGAVVQKFVNNSALDNVGFNASTGIDLSQAVNRILPRMRGARIFGTYMYT
                     PSAPAFGAGSLGGGAGMGGGGFGGGGFGAGGIGIAGPIDSGLLTQRIRTTMFNAGFAN
                     SYSLSPTTDFQTTYTYSQLSFGGSFTPSTTVSGQAQNTVFDTTTHSISAGPTSQISAI
                     DTLTVKYTFMQMSQAQFGDYATHGGTLGWGRAWTKELSSSLNGGLTLVEPIPDLSTSG
                     GQRRIPATMFPTGGFSVTYASGSSFLRKLGSEIQEATGTSGAGSVGGGGFLPLLAGMN
                     MPGGIAAPGSYRVTLMYNLGVFPSFVQSAGPIYTHTILLGGTAGITDRLSAQALFNYA
                     RSSYTSEAIGTTFSTYGTTVSLNYLITPMLTARLSHQWLKFDNQTSGVSAADFGFAKQ
                     VVLLGFTYAYSPRGDFFRSGAFWEGPSGASSSAESGVSKSGSGGVDTKK"
     gene            28278..30632
                     /locus_tag="PK224_07520"
     CDS             28278..30632
                     /locus_tag="PK224_07520"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRB15622.1"
                     /translation="MNTRTLSPEDYWRAVVSRKWFVISAILVSLSIAGVVCALMPKIY
                     QSATKMWFEGAKIEESIVSGPTPAGSGYSPTLDDRVMEVRQFVMGRKTLGQIAGEFGL
                     FGYEKDHPDAPESENAIRAMRGSIKVEPTKDKLFITLSFSNEDPIIARDVTSRLSDLF
                     IEETLKDRERGVEAAEDFLGLELKHAKAELEIKEKIISEFKQQHLGELPQQIDANLRK
                     LDRLQDDMRSQSEQAQNLANRLAQIDKSIKDYEETGEISSDSPVGGTSKRNKDPRLGR
                     IKELERRLVELSSIYKETYPDLVQVKEEIKKLREMTSAQYRDLLPDSEGVEEPAVGKK
                     SKRKAIDPYHAELLKQREEIVLELDAVKRHQAHISSEISQYERRVEHTPEREQKLKTL
                     ERDYENLQKNYQSLLDKKLSAGMQKSLAQGRKGAKFSIVDPAYTPVVPVVPNIPLIMA
                     AGLVLGCALGFGGAVGLELMGRGFRSAEEVELTLGLPVIASIPLYESAFGGSMQTVRA
                     LSGKSRNAAVLLPRYGKQGESLEISGGLPVASVSKSRGANGKLNDPTPGLELVSMWRP
                     LSFVAEQYRVAATRLELMTGDRKSTAIVVTSAVMGEGKSSTALNLGYVLAKDLDRRTI
                     VIDCDLKRPMQHIYAGVWQQPGLAEVLRGTKVVEDCLQRLGEAGPWILTAGAVGDNPL
                     ALSKMHQLADLIAELKEKFDYVIIDAPPVLPLADMQVLASMGDLLAYVVKASMTGRDV
                     VQKALRVIGDTANVGIILNGLDAHTTPYYMQQEYYREAHHEQLK"
     gene            30616..31683
                     /locus_tag="PK224_07525"
     CDS             30616..31683
                     /locus_tag="PK224_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HRB15623.1"
                     /translation="MNSSSKSEQEIGLEPVVTQPPLVSVSVSFPKFTRRVLILGVGPL
                     ARDLCQTLLSKRTGFTEVVGFLDKDASRVGERLVNPSIIGTYDQLFEIAERYQVHTVA
                     VCLEDRRAVLPVQTLLDMKAMGRDVVDGHYLYEEESGRLSIDHLKPSALIFSTGFRRR
                     LVTMLLKRCLDVLVAIVGIVALVPLVLLLSILIKLDSSGPVFYRQMRVGLRGRPYMIW
                     KFRSMRQDAEQSGARWATSEDPRVSRVGRWIRKWRLDELPQLINVMRGEMSLVGPRPE
                     RPVFVQELRNTIPYYDLRHTVRPGITGWAQTRFRYGASKEDSHVKLQYDLFYVKNLSL
                     ALDLRIVVHTVKVMLMGEGAR"
     gene            31693..32580
                     /locus_tag="PK224_07530"
     CDS             31693..32580
                     /locus_tag="PK224_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HRB15624.1"
                     /translation="MPDAIPRHCLSFDVEEHFQVSAFETPMRRRHWGQFESRVEDNTE
                     RLLGLLENRGVRATFFILGWVAERYPSLVRRIASGGHEVASHGYAHELITSQTPSTFR
                     EDIRKAKGILENILSQPMLGYRAPSFSITKDTMWATQILVEEGYVYDSSIFPVLHDRY
                     GVPSANPEAHQLLTASGLLWEVPPSTVKCLGVRLPVAGGGYFRLYPYALLRALLRKLE
                     AEGASLVMYMHPWEFDPDQPRMEGSLVSRMRHYLNLDKTEIRMRELLQDFSFAPIREV
                     FPQIEQMHSARLVSQVSGV"
     gene            33148..33966
                     /locus_tag="PK224_07535"
     CDS             33148..33966
                     /locus_tag="PK224_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB15625.1"
                     /translation="MAVLVIEPRTGYVQVGWRELWAARELLYFLAWRDLKTRYAQTAI
                     GAGWALMQPLLSTLIFTLVFSYLAKVPSDGLPYPLFAFAALLPWSLFARSLERSTLSV
                     VTEGGLIKKVYFPRLIIPIAATFINLVDFTVGLLILIGMMTWYQVLPQWTVLFLPLFV
                     IVALLTALSVSLWLSALNVKYRDVASVVPLMTQLWMFASPVLYPASLVPESLRWYYGL
                     NPMAGVIEGFRWALLGKSAPDWTMVAVSLGVVGFLLIGGVMFFRRVERTFADLI"
     gene            33967..35238
                     /locus_tag="PK224_07540"
     CDS             33967..35238
                     /locus_tag="PK224_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB15626.1"
                     /translation="MSNTAVSVTGLSKRYRLGTTHAQDCSLAGALTRGLRRLIGGQPA
                     PPQTHDTLWALRDVSFEIKKGEVFGVIGTNGSGKSTLLKILSRVTEPTKGRALIHGRF
                     CGLLEVGTGFHPELTGRDNVFMSGAILGMKRQEIARKFDEIVAFAEVEQFIDTPVKHY
                     SSGMYVRLGFSVLAHMDPDILIVDEVLAVGDVRFQKKCMGKMEDVGQHGRTVILVSHD
                     MPAITRMCSRAILLNKGEIVQAGPAHEVVNHYLHAGHIQPAVEWLDPTQAPGNDVARL
                     RALRVRSEAGQVTDVIDIRKPMRVEIEYDILKPGQMVVAQFGFNTEEDVIAFIVNDRD
                     PVWYRRPRPVGRYISTAWIPGNFLSEGTMVVGGGIFSEDPWHEHCDVPRAVGFRVVDP
                     GTRDTARGDVTGRWEGVVRPLLRWTTEHSSL"
     gene            35868..36638
                     /gene="rfbF"
                     /locus_tag="PK224_07545"
     CDS             35868..36638
                     /gene="rfbF"
                     /locus_tag="PK224_07545"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HRB15627.1"
                     /translation="MKAVILAGGLGTRLSEETVLRPKPMVEIGGKPILWHIMQIHAVY
                     GVTEFIIALGYKGEMIKEYFLNFFAINNDLSVDLSTGKTTIHDGNQPKWTVHLADTGQ
                     TTQTGGRVRRLKKWLENDETFMLTYGDGVADLNISKLLEFHRSHGKIATMTSVRSPAR
                     FGRIGFDGDRVTEFFEKPESGEGWINGGFFVLNTKALDYIDGDHTAWEREPVERLAHA
                     GEMVGYKHYGFWSCMDTLKEKSYLEELWNSGKAPWKLW"
     gene            36724..37857
                     /locus_tag="PK224_07550"
     CDS             36724..37857
                     /locus_tag="PK224_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB15628.1"
                     /translation="MTADTSLFAQAQAWLDEDWARMEKTLGPRLEAFRGKHILVTGAA
                     GFLGFGFLHFFSYLNRNLLKTGRLSIVAADNYIRGCPRWLTELVAASPDIQLLRQDIT
                     KPWTGPAGDRFDFIIHGASIASPKVYRQYPLETLDTNISGLRHMLELAKSHRAESILY
                     FSSSEIYGDPPAHEIPTNEAYRGNVSCIGPRACYDESKRLGETLCYLYHQQHGVRAKI
                     VRPFNNFGPGLRLADGRVLPDFCRDILADRDIVLRSDGSPTRTFCYVSDALTGYLLTL
                     LSSNHAEPFNIGSDSPEISMRELGRMLLEVAGSKRKVIHTPSEEVDYLTDNPNRRCPN
                     LAKSRSLLGYTPLVDLRFGLSRMLQWYKQFVGLEELAKDERVG"
     gene            37872..39233
                     /locus_tag="PK224_07555"
     CDS             37872..39233
                     /locus_tag="PK224_07555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRB15629.1"
                     /translation="MKISVMGTGYVGLVSGTCLAERGHQVTCIDIRSEVVQEINAGRP
                     PIHEIGLDNLLRSARDKGMLSATTDAKTAILDSDVTLICVGTPTVDGRMDMSQIVAAA
                     KEIGSALASKRGYHVVAVKSTVLPGTTEGPVKAAIESHSGKKVGHGWGLCMNPEFLRE
                     GRAVEDFRVPDRIVVGATDSMTAEVFLNVYADFTCPKLVTTPRTAEMIKYVSNSLLAT
                     MISFSNEIGNMCSAVPGVDARLVWKGVHLDRRLTPINGQVGGAAGVTEYLWHGLGFGG
                     SCFPKDVAALRSFGRTVGEQTRMLDAVLDINTDQPLRMVALLEKELSLAGKTVAVLGL
                     AFKPGTDDLRESPALPLVAELRKKGAKVLVHDPIAMPHAKKRPEFRDVVFAEDWAEAL
                     RKSDACCLVTAWPEYRNIQPTNFQKLMTRALVIDGRGVFEPAAMAAAGVVWRGVGYTP
                     EST"
     gene            39299..40534
                     /locus_tag="PK224_07560"
     CDS             39299..40534
                     /locus_tag="PK224_07560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB15630.1"
                     /translation="MLRDGCRFCGTLLQHVFLDLGMSPLANSYLKAHQLNQMEPFYPL
                     RAFVCERCLLVQLEEFESPDHIFSDYAYFSSFSDVFLQHAKDYVDMAVERFSLGAHSY
                     VVEIASNDGYLLQNFVARGIRVLGIEPAKNVAEVAVKKGVPSCVKFFGTQTARELVAE
                     ERRPDLIIGNNVLAHVPDLNDFVGGLKILLAPEGIITIEFPHLMHLMGLNQFDTIYHE
                     HFSYFSLTTVNQVFAKHGLTIFDVDEISTHGGSLRIYARHDEHHSKPVTERLMALKKK
                     EEIQGFATLAHYLSFSEKARETKRALLEFLIRAKREGKSVVAYGAAAKASTLLNYCGV
                     RQDFVDYLVDRSTYKQGHWLPGVHIPIHDPERIKETKPDYVLILAWNLKEEIMKQMSY
                     VRNWGGQFVVPIPEVRICP"
     gene            40655..41914
                     /locus_tag="PK224_07565"
     CDS             40655..41914
                     /locus_tag="PK224_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HRB15631.1"
                     /translation="MYRFISELYPICRSITGQGIRDTFGRIQQHIPLSITAVPSGTQV
                     FDWMVPLEWNIRDAYVADRQGVRVIDFKQSNLHVVSYSIPTAARMPLRELKAHLFTLP
                     DNPDWIPYRTSYYKESWGFCLSHRKYLDLRDEDYDVVIDSSLKPGHLTYGEYYLPGDT
                     TEEVLISCHACHPSLCNDNLSGIAVATFLAKSLQTGSRRYSYRFLFLPVTIGAITWLA
                     LNQQKVSNIKHGFVLTGVGDEGGFTYKKSRDGNADIDAAFAHVLQHSGESFAVTEFIP
                     YGYDERQYGSPGFNLPVGCFMRRANGGYPEYHTSADNLDFVKPTSLSRSLQVVQSVVE
                     VLESNRGYVNTSPYCEPQLGKRGLYRTIGGNVTGQSAEMALLWVLNLSDGAHTLLQIA
                     ERAGLPFSTIKLAADALEGQGLLRPAG"
     gene            42073..43014
                     /locus_tag="PK224_07570"
     CDS             42073..43014
                     /locus_tag="PK224_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRB15632.1"
                     /translation="MNNTIYYDAQLTDDERRKLIFEGQLVAYSPREHSLALVAHARKL
                     IEEAFAPLDPETAQYHLPVEQYAEVLGKLKPHFIHHPDSKKHLRALLAEMGADPEKTY
                     FDVPKMRSSTSDNYLTTGIAYAWHPHRDTWYSAPPCQINWWIPIYDIRSDNAMAFHPK
                     YWNRAVKNDSRSHNYYEWNKQHRGGHVSQFLKADPRPLSKPIETIEIDPQIRIIVPAG
                     GIILFSAAQLHSSVPNTSGKTRFSIDFRVVNVDDAAARRGAPHVDEECTGTTMRDYLR
                     GTDLSQIPAEIVALYDDGAQEDGELQYKPKDVSTPSL"
     gene            43028..43957
                     /locus_tag="PK224_07575"
     CDS             43028..43957
                     /locus_tag="PK224_07575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB15633.1"
                     /translation="MPNLNRWRGERILVTGGSGFLGSHLCQRLLELGAEVHATSRVER
                     TSEPGGPIWWQVDLTAIDRVRKLLTDLKPMVVYHLAGSAGAKPDLDLVLPTLEGLVVS
                     AVNILVAGTEAGCGRIVMTGSLTEPAWGEHAPIPSSPYAAAKWTSSAYARMFHLLYHA
                     PTVVLTPFMTFGPGQDRSKIVPSVILSLLRRESPRLSSCLWEVDWIFIDDLVEAFLAA
                     AAVPDIEGGCFDVGTGTNRTVRSVVERIFLLMGEQTQPLFGMLPDRPSEPVRSADTMQ
                     TYQLLKWRAQMSLDEGLRRTIAWYTTHAAMGGA"
     gene            43954..44955
                     /locus_tag="PK224_07580"
     CDS             43954..44955
                     /locus_tag="PK224_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15634.1"
                     /translation="MTAAAMQQVLKWYAKRKIRELCPLSIADRLLGEQGAIHQLWSIG
                     IVTGASPLRLRHAPGGLNPVLTREDISDVRAMFVADPFLLRVGDLWHMFFEVYNFDAD
                     RGEIAWATSKDGFAWTYQKVVLQESFHLSYPYVFEWHGQYYMIPETHQAESVRLYEAR
                     SFPTKWECTDTLLRGHRFNDSSIFYHHGRWWLFSETNPTLAHDTLRLYHAPDLRGPWQ
                     EHPRSPIIQENPHIARPAGRVVVVGDRILRFAQDCFPVYGTRVQAFEITELTPSTYRE
                     QPASRGPLFGPSWRLWTQGGMHHIDSHPLDSHQWIAAVDGWRQVGWPIPEEWQRATC"
     gene            45186..46157
                     /locus_tag="PK224_07585"
     CDS             45186..46157
                     /locus_tag="PK224_07585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15635.1"
                     /translation="MAQTFVDLGYHVDVIHCENQRFIPWKPYDIVVDTRLNLQRLQPY
                     LPSTCIKILHCDTAQIVYQNAAEMGRMLAFQRRKGLTVPPNRLETPHLGVEHADYLTT
                     CGNEFTVNTYTYADKPIFRLPMVVQQMWPWPETKDFDLSRHRFLWFGSRGMVHKGLDL
                     VLEAFARMPECQLTIVGPVRNEPEFVNVYRKELFHTPNITYVGWLDKSSEEFRIVLEQ
                     SLAHVFPSCSEAGAAAVVETMAAGVIPVVTYEASIDVENFGFLLEDASIETIMRQVRE
                     IAAMSVDELRRRAKKAWEAAHNNNTPEKFERSYRATIEMILAKHGRR"
     gene            46250..47701
                     /locus_tag="PK224_07590"
     CDS             46250..47701
                     /locus_tag="PK224_07590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB15636.1"
                     /translation="MAKLGRQLGKLANFVLLLGAALCAGVFAFLVWRHGMSLRYVVFV
                     GLAVILALGVRMPESLRINSVLVGVSTCVAVYLAELLLAYSGTAITSLGAQAWLSFPQ
                     DANVRVAAERMKTVQETHQKFDARSRLEVILDMRAHGVNAYPDVFPMVLFAPSSSDSI
                     QSVLTSQHEEFLPVAGMAATTTVFCNESGEYVVYESDEHGFHNPRGMWEHRPVELLAL
                     GDSYTHGVCVPSDKGFVAVVRGHHPNTMNLGVNSHGPLTSLATLKEYGPVLKPKLVLW
                     FYYEGNDLRDLDGWEKNSPLLMKYLSSSFSQHLFERQPEIDRSLRDYLDAAMAKATAP
                     ISLENILKLHHLRHAVQSFYERRPAEQGFPAELLEFLRHSGAPAAPEDLQLFERILAE
                     AQATAKTWDGRVVFVYLPTWERYRLPELASKDRDNVLGIARRLKLYVMDMHEVFVTHP
                     DPLSLFPFRRYAHYNEAGHKLVGEEVVRQLGKL"
     gene            47844..48680
                     /locus_tag="PK224_07595"
     CDS             47844..48680
                     /locus_tag="PK224_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HRB15637.1"
                     /translation="MNTHQKKVAVVVPMHNRSELTPDEQISFQHLTHYLHAYDKYLVV
                     PNSLNIDLPGCSLKRFGDEYFGSVAANTRLLLSETFYRSFSEYQYILIYHLDALVFSD
                     QLEAWCDTGLDYIGPPWIHCADSPWVKEARVGNGGLSLRKIESFLKVFQSDVYWMEPE
                     EYWRERYAGKPAYVRMLNLPRRFAKRLSWLNNARLEMSQWHLRPDGTKNEDHFWSDRA
                     KHYVPDFRVASVELGLRFAFEVAPRLCYDMNHHQLPFGCHAWPRYDRSFWEPYLITPH
                     AA"
     gene            48718..49800
                     /locus_tag="PK224_07600"
     CDS             48718..49800
                     /locus_tag="PK224_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB15638.1"
                     /translation="MARRFAMVVALGVATVVSAAIMLGLGELTIRSIHLLRDGIPFFE
                     SVDGGRIGPISLDQELGWKATEHYQETLVEKTNAGRPYSVRRSQKQYGFRQFGALDSK
                     KIRLLVIGDSFTHATAVSDDRTYHALLAQLLDVEVFAYGAGGYGTLQELMILDRYIDT
                     IRPDVILWQYCANDFINNDNELERLSLVNNNGWVRPYLQKGQVLLLSPKESSLQVREW
                     INQRSRFLYFVVSRIDRLRAVRTRDTVEVAIESEGMRHPGFLRAVGVTDELMGRVRKK
                     VGNRPIMAFNCVRAEPYDQAFRDISARHGIAYWDDVASVVLEAHHRGEDVYAADGGHW
                     NERGHQLAAEALASHFRAEMRTGARP"
     gene            49849..50919
                     /locus_tag="PK224_07605"
     CDS             49849..50919
                     /locus_tag="PK224_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB15639.1"
                     /translation="MKRMLVTGSSGLIGSEVCAYFHSQGWSIHGVDNNTRATFFGPQG
                     DTRWNQRRLQADLKQFRHHELDIRDRKGALALIQELKPDVIVHTAAQPSHDLAAKIPF
                     DDFDTNAVGTLNLLEATRLHTPGAVFVHMSTNKVYGDAPNELPLVEQDKRWDYASPND
                     FDGITEHMRIDQSKHSLFGASKVAADVMVQEYGRYFGMKTCCLRGGCLTGPNHSGVEL
                     HGFLSYLVKCNLEGKKYSIFGYKGKQVRDNIHSLDVARFIHAFIENPRSGEVYNLGGG
                     RGNSCSIYEAFEMIEAISGKKMLYEYVDKNREGDHICYISNLKKMRTQYPGWGITKTL
                     KHIFEEIHQSWLKRAAAVNVPI"
     gene            50962..52200
                     /locus_tag="PK224_07610"
     CDS             50962..52200
                     /locus_tag="PK224_07610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB15640.1"
                     /translation="MKVLVISAAYPPMHAGEATNTYHLCRQLTERGIDIHVLTSVGNV
                     GTDDPRIRVYPIIQTWGWLELLRVRSFLKNCSPDAVFLMYIGLMYKFHPMVTFLPTLS
                     KGLFPNVPFVTRYESAFVGADPSKTGIVSRLFRKLMVQWAGGKDVVYSSGTLLRDSDA
                     VIALCERHRAMLLEEWPPVSQKVALIPPPPNLFIASNAGGMARERGRTRLGVTAGEFV
                     VTFFGYLYPIKGIETLLRAFASVSAQRPEARLLFIGGKVGLDVEGGVSYFDEMQALAK
                     ELHIDGLTTWTGAFKSEEEDASLLLHASDVCVLPFLEGVQLNNSSFASMVAHGLPIIV
                     TRGPLMDKAFVHGKNVLTCEPKDHPAVTKLLLEVMDRADLRERLRSGAVKLAAEWFSW
                     ETATEKTLTALRPRLSDKVV"
     gene            52227..53135
                     /locus_tag="PK224_07615"
     CDS             52227..53135
                     /locus_tag="PK224_07615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15641.1"
                     /translation="MPRVSVVIPTYNCAKFLGRTIDSALRQTYRDFEIIVVDDGSTDG
                     TQALVAAYEESVRYVYQPNQGASAARNAALSRASGEFIAYLDADDLWRPDKLSRQVEF
                     LDAHSTCGFVHTEVSVIDEQDTVLHTCFNQDTKRPIPQGQCVRDLLLRSHIQTLTVVE
                     RRTAFDDAGKFDPRLPIAQDYLHWIGVGLQGYGVGYLPEPLGQYRWRAGSLMSSQRRL
                     LGDFVKIYEILSTEYGLERSQGPEIMQLVQAQLYATQRQLAYLERRECSGAAARQRLG
                     ALIREWPLNLELYVDFAKTYVSRQVS"
     gene            53205..54368
                     /locus_tag="PK224_07620"
     CDS             53205..54368
                     /locus_tag="PK224_07620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB15642.1"
                     /translation="MNIMLVTPWRPSLTGGISTVVARLTGEFQKKGHNITIFVADREN
                     RLRQIEALDEIPVYGMYLRSPVSSQYPLRAMVMCCIWFPITMVQLIWLARKKRLDAVL
                     IQYPLPAMFYFGILKRVFGGTLFITYQGNDAHDLCLWDRREQRLVRFLLEKADLVLGV
                     SRTLLAKVESVLQGVHLRRSRQLPNGAPLDVIRAVKAGEAGSDLPPSYLLTAGHLIQR
                     KGIDLVIAALREAKERGVVLQLVVAGDGPERENLIRQSREQGVSDQVRFLGNQSHRQV
                     LSLMKTCLAFVLASRAEGMPLVIAEAMACGKAVIASNIDGVPEIVHDGTTGILVPAED
                     SGALATGLIRLCSDVAFRETLAKQGKEWACREYNWEAIAHRYLGFIEECQAHR"
     gene            54493..56337
                     /locus_tag="PK224_07625"
     CDS             54493..56337
                     /locus_tag="PK224_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HRB15643.1"
                     /translation="MNILGISAFYHDSAACLVRDGEIIAAAQEERFTRKKHDPGFPHR
                     AIDYCLREGQIELKDVRHLVFYDKPLVKFERLLETYLAFAPRGVQSFVAAMPVWLKEK
                     LLLKSLLQKEFLQHAPDLTTSSLPQILFSEHHESHAASAFFPSPYEKAVVLCMDGVGE
                     WATTSAWLGQGNTLTPLWEIPFPHSIGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPK
                     YVKAIYEHLLDLKPDGTFRLNMDYFNYCTGLTMTGGKFDDVFGGPPRKPESKLTQREM
                     DLARSVQEVTEEVMLRLSRTLHRETGVDYMCMAGGVALNCVGNGRILREGPFKGLWIQ
                     PAAGDAGGALGAALTAWHQYEQQPRKVTSGKDGIKGSYLGPCHSNEEIEAYLKKVDAP
                     YVRLDENQLYARVAEELAAGKVVGWLQGRMEFGPRALGGRSILGDARNRDMQSVMNLK
                     IKYRESFRPFAPSVLRERVSDYFVLDADSPYMLLVAPVVEKRRLPVSSAQDGLWGIEL
                     LNIPRSDIPAVTHLDYSARIQTVHQDTNPRYYALLKAFEAKTGHAVLVNTSFNVRGEP
                     IVSTPEDAYRCFMRTEMDVLVLENCVLLKADQKPLEGDSDWKKEFELD"
     gene            56374..57384
                     /locus_tag="PK224_07630"
     CDS             56374..57384
                     /locus_tag="PK224_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB15644.1"
                     /translation="MRILITGGAGFLGSHLSDLLIGQGHDVIALDNLITGRAENISHL
                     IGNPKFSFVKYNVCDYLHVDGQLDAVMHFASPASPQDYLEMPIATLKVGALGTHKALG
                     LAKAKGARFLLASTSEVYGDPLLNPQPETYWGNVNPIGARGVYDEAKRFAEAMTMAYH
                     RYHGVDTRIVRIFNTYGPRMRPKDGRVVSNFIVQALQGKPLTVFGDGSQTRSFCYVDD
                     LVRGIVALLMVKSDKSVAERTDRTKFLTQKPDAILDSIHDPVNIGNPRELTVRGIAEI
                     ILKITGSKSVIEERPLPADDPKVRRPDITRAKSLLDWEPKVELEDGIRKATEYFRQVI
                     AK"
     gene            57437..59332
                     /gene="asnB"
                     /locus_tag="PK224_07635"
     CDS             57437..59332
                     /gene="asnB"
                     /locus_tag="PK224_07635"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB15645.1"
                     /translation="MCGIAIAIGLNGRPIERVAVERMAKSLRHRGPDDGGIYLDGAVG
                     MGFRRLSILDLSEAGHQPMVSEDGQYVLVFNGEIFNYVELRSELRQLGYQFRSSGDSE
                     VLLAAYREWGRECLSKLNGMWAFVIYDRRHRLIFGSRDRFGVKPLYYSRSSDVMQFAS
                     EIKALRASGYQPGEINWRTAARFLFEGRLDSQVETFYQGIEQIPPGSGFELTLDGTWH
                     QWSFWSLDELPLSASKNPAATFAELFEDAVRIRMRSDVPVGVCLSGGLDSTAIICAAA
                     RQRGETAVGQSESLQAFCYMAKEFDESKYITDTLTQTRAQLRQLETSPAELWSDLRQL
                     LWFQDEPVHTMTAVVGYQLMRLAASHGIRVVLNGQGADETIGGYSSYFQDYWVSLLRQ
                     GRVREAWQAVTGYTEGHGGNSRQRLVAAAARCLSWEMYKFHAYRSWAQARRQARLRQN
                     PWFSEDLTKHFINEDVPPASTTLSNSLKQSVVSAPLPLYLRIEDRNSMAHSVEARLPF
                     LDYRLVSFVSGLSDDWKIRGPWNKYVLREGMRGRIPESVRSRVDKMGFPTASKKWFAH
                     DLYEPLRDILGSQAVRERGIYNIGALTADLERHHRGESDFANRLFHVAEFEIFSDLLR
                     HDSGLAA"
     gene            59500..60882
                     /locus_tag="PK224_07640"
     CDS             59500..60882
                     /locus_tag="PK224_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15646.1"
                     /translation="MKRVLIIAYYFPPVAASGAMRPLGFCRNLPTFGWQPYVLTTSPE
                     CVYPAHQVDEKLGERVPATVGVTRVPYTDRLQQILYYRDVLRRMVKRQVGQDRTGKLQ
                     TTQAPPSPPQSEAGRSTVKDFLLDWAFAFPDRQCGWLRQVVQHFEQFDEKQIPDVVFA
                     TGGPWTSFLVGSTLAKQFNRPLVLDYRDPWNCNPYYSFSSRFLTRKSQAVEARVCKAA
                     SHVIANTEELRQRLIEEYGELRGRCTWIPNGFDRDVLPLGQIPAEQLDGLSPSTGYEL
                     CHFGTVYGKRTPRVLLQAMWELFQEGWLKPEAIRLRFVGGWDSTDQECERYAAELEKR
                     SFLRREPSISHSLCLQEMQRSSVLLVLQPDSPLQVPAKIYEYVATGRPLLLIGGEGAT
                     ANLVTRHALGVSSPNQLEAIKVLLNELATGARKLAQPDVTRVNRFDYRSLTGDLAAVL
                     DVVIGAKECS"
     gene            60902..61939
                     /locus_tag="PK224_07645"
     CDS             60902..61939
                     /locus_tag="PK224_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HRB15647.1"
                     /translation="MVSSILGRTNAEILAWESYVLSSPLATGYHQSGWRRVIEEAFGH
                     PTHYLVVHGQDGVLQGVLPLVFLASRGFGRFLVSLPFVNYGGVVASSAEAHRFLEARA
                     IEQARELNADHIELRHQALMDTAWVATERKVSMRLPLPSSYEQLVKGFPSKLRSQVRR
                     AQKEGMTARVGGSECLDEFYSVFTRCMRDLGTPVYAKGFFAKILEVFPKESNICIVSH
                     GQTPVAAGFLYGFRSSLEIPWAASDKRFNKLAPNMLLYGTVLEYACQRGFQVFDFGRS
                     SPDSGTYRFKEQWGAQPHKLYWYYWMKDGRPVPQLNPQNPKFALAIRLWQKLPVVVAN
                     LIGPYIVKHLP"
     gene            62005..63213
                     /locus_tag="PK224_07650"
     CDS             62005..63213
                     /locus_tag="PK224_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB15648.1"
                     /translation="MGLVHPHETVGQLQAEFRQYFGVKHVWFVSSGKAALSLIFRALH
                     SLSGRSKVVLPGYTCFSVPSAVVRARLSVALCDVDPLSLDFDFAQLSQVADSNVLCVL
                     ATHLLGIGVDVPRVVELCRQRGIFVVEDVAQAFGGERDGVPFGAMGDVSFLSFGRGKN
                     ITCGSGGAILTNDDRIGEVLAREYALLPEMSLADMLKNWLEVAVTKVLLNPSLYWLPA
                     GLPFLKLGETKFYTDFPITRLDPIRAGLLRRWKRRLANSTTSRVAHSEQILRSLTLSN
                     VQTIKPSGRGQSVYLRLPVLMRSRQEKDAVCRTSAEQGLGISPLYPSSLQYITKLRDT
                     LSSQDVPQSTMIAERLVTLPTHELVSDSDVVRISTVIQAAQRGDGAATIRRPDITNGQ
                     RHAPELPRNI"
     gene            63274..64413
                     /locus_tag="PK224_07655"
     CDS             63274..64413
                     /locus_tag="PK224_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB15649.1"
                     /translation="MWFVEWIFWGSLVFIVYAYAGYLLALLVLSCFRNRPVLVGDIQP
                     SVSFVITAYNEEARIKEKIENSLHQQYPPERLEIVVASDCSSDRTDDIVRSYAALGVR
                     LVRAQERRGKEAAQKLAVSQTNGEILVFSDVATTLPPQGIANIVKSFSDPTVGCVSSV
                     DQFVDAQGNLSGEGAYVKYEMVLRHLETKVNTLVGLSGSFFAARRTVCSPWADDLQSD
                     FNTLLNSMKAGLRGVSDPASVGYYKNLTDEKKEYQRKVRTVLRGIAVLMRSLPMLNPL
                     RYGIFAWQLFSHKLCRWLVPFAMIGTLGSNIVLAISSSPYRVLLLGQILFYAIAIAYA
                     GCSWMPKSNVFRLPSFFVLVNLSIIDAWMRYFRGDRVFRWEPSKR"
     gene            64477..64872
                     /locus_tag="PK224_07660"
     CDS             64477..64872
                     /locus_tag="PK224_07660"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HRB15650.1"
                     /translation="MDKMVEKKELRNFGLIVGGVFILIGVWPMIRYGEGIRLWAIVPG
                     SLLVPLGLAAPTILAPLFKVWMKVGHVMGWINTRIILGILYFGLITPMGVIMRMFGWD
                     SMRRALSRDAESYRVVRQARPRNHMTRQF"
     gene            64889..65041
                     /locus_tag="PK224_07665"
     CDS             64889..65041
                     /locus_tag="PK224_07665"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HRB15651.1"
                     /translation="MGEFLTELWAFMKERKKFWLLPIIVMLVLLGSLIVLTQGSAVAP
                     FIYTLF"
     gene            65175..66122
                     /locus_tag="PK224_07670"
     CDS             65175..66122
                     /locus_tag="PK224_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB15652.1"
                     /translation="MYHRVLTKDEVARYPVQPGMYVLDSVFAQQMSFVRSNFTVLSLQ
                     ELLDLWQRGEWSAHARYCVITFDDGWLDNYRHAYPVLKQLRIPATIFLPTDYVGRNEW
                     FWPDQLAVLCKVIADRKQRQEPLAAVEGVLSGFLSEDASRLVEASVRQEQLGDEIIER
                     CKHLPIERIRELVQALVAELRVTLPLDRVIVNWDEVREMSRGGVSFGSHSCTHRIMTT
                     ITLDEVSEELVRSRQVLFDQGVNFVPVYCYPNGNSDDSIEDLVKAHGYEAAVSVRMGF
                     EGSCPENRYAIRRVGIHNDISDTIPLFSFRLFGPQPSSV"
     gene            66175..67362
                     /locus_tag="PK224_07675"
     CDS             66175..67362
                     /locus_tag="PK224_07675"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRB15653.1"
                     /translation="MKVLITDGNERAALAVTRALGGEQVEVIVGAESQRSLAGSSRYC
                     RQSIAYPSPYQEPERFIATLMEAVQTHRVDALIPLSDIAMHVLGPEKAQFEKYTHFPA
                     PSPQAFQEISDKYRLMQQAKAAGVAIPDTLFVPDGRVEEVLREIADFPVVVKPGCSLV
                     KDGRRWTKTSVCYAESPEELLRLYRERAYLRQPSLIQRRVIGEGQGLFVLMQEGMPLG
                     MFAHRRLRERPPSGGVSVLRESIALPKAMVEATLKLLQPVKWHGVAMVEFKVDTATQR
                     PVLMEINGRFWGSLQLAVDAGVNFPLHLLNMAMGVCETIPENGYRVGVKSRWLLGDLD
                     QLVMRIRKSDRALNLPPGAPSRLQAVLSFCRFFDRNTFYEIERIDDLGPSRFEVMRYV
                     KLA"
     gene            67417..67959
                     /locus_tag="PK224_07680"
     CDS             67417..67959
                     /locus_tag="PK224_07680"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRB15654.1"
                     /translation="MKRAVNEALDHWRYRSLVNSQAFPSSVRAILVVCKGNICRSPLA
                     EAYLKHRVEKHGLPIAIESAGLETSFGKAAHPLAQVVGTQGGLLLAKHATQQLHKEQV
                     ERADMIVVMEWRQRRRMLKLYPQARQKVFLLRQFYDQSVREVADPYSGTLEDFQTCFS
                     MIKQACDVLVMQMLASGKQR"
     gene            68025..68771
                     /locus_tag="PK224_07685"
     CDS             68025..68771
                     /locus_tag="PK224_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB15655.1"
                     /translation="MSLRTRLFNIYWTLRGAIVPTLRYSQYSYEESLKRYVTPSVEWV
                     EIGCGHSILPSWRANEERQLAKTCKAIFGIDYDLPSLKAHPNITKKLRGDVTKLPFKS
                     EAFDLVTANMVVEHLDNPDEQFREISRILKSKGVFLFHTPNAYGYGVILSKVVPEWLK
                     GKLIYLLEGREEHDVFPTHYKANTEAQVRALAQANGFEVLQLDLLATDAIFAMLPPLA
                     ALELIWIRLLMTQPFRNLRTNMIVALRKVV"
     gene            68821..70185
                     /locus_tag="PK224_07690"
     CDS             68821..70185
                     /locus_tag="PK224_07690"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB15656.1"
                     /translation="MMVVAKRQTLMPAEKALHLVGNRPLPTESNVGFYLVMLALLFEF
                     GRPQDIFPPLKVVPIPTLLDVSIFLAVLVSGKATIANEQTKLWLILLAFMALWVPFAT
                     NNFWALMIFKEMTLYLFLYLGIVTFVNTTSRMQKLIFMWLGVHGFLGINGILHHGQGV
                     GGWLGDENDFGMEMNVAVPVAFFMYQAASSQRAKLLYIVLLGLFIMSVVATASRGAFL
                     GLLAIGAYCWFYSPKKIMSLILGTCLVGLVLLAAPQEYWDRISTITDDKTMEEGTAGQ
                     RMFTWGIGWEMFLANPAFGVGQGNFPWTIGEYLGGRTWQTKSLAGRQAHSLYFTLLPE
                     LGLVGVILFSSMVYFNYRDLRLKEFHRHPSRKIIGGLGERKDTEISRAFMFGNAILGG
                     MIGYLATSTFISTLYYPTFWTMMALAVSLRNTTMNSSAPSNEEVRQLIRPSISRHAKD
                     IRHI"
     gene            70330..71340
                     /locus_tag="PK224_07695"
     CDS             70330..71340
                     /locus_tag="PK224_07695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB15657.1"
                     /translation="MAVPKKEVLYIWILLSLSVLCAAILGEMGLRLMGYKGAPESLIA
                     NIRPIDDPILNWRYLPGSEVQTGKIKRKYNKSGFRDVDHNVEKAPGVKRILVVGDSVT
                     EAGDVEWEAVFSSRLQADLGRSYEVITLAMGGLNTPQEIHLFEKEGLAYKPDLMILNF
                     VLNDADFYTEYNATQRYQAEKDSVVGVLGISINPAFKRTLKSSALVYFLKQRFEHAIG
                     MFLGKEEIGYFENLWGKAENRQRVTNGFDRLWVMSKDSSFPVLVVIWPILTEFSRYKF
                     EHIHQWVREEAEKRGFQTLDLLPHFAKTSYRNLQVTAEDNIHPNGLGHKIGADAFLEW
                     IR"
     gene            71445..>72132
                     /locus_tag="PK224_07700"
     CDS             71445..>72132
                     /locus_tag="PK224_07700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15658.1"
                     /translation="MSSDIEKIPVLYLSSSSGPGGAERMLCSLAASIDRARFRPIVGL
                     FRPGWLKEQCEQQGIETHVFPTGGMLHGAWISGCTRLVREERVALIHAHEFDANVHGT
                     LVAAITGVPIVATVHGKHYYWTKFRRRAAYRFVSRFATMVAVSQNLKDFIGQKVGIDQ
                     ARIRVIYNGVKPLPEYFAGNILGCRVELGIPEGDQIVGVVGSLYPVKGHRFLVEAIPH
                     VLAKFHHTTFL"
BASE COUNT        15709 a        17141 c        21719 g        17563 t
ORIGIN      
        1 cccttcagcc acacatgttg aaagcttcac tcaaacgtta ttttctcacc ggttttgctg
       61 cctgcgaggg gcgtgtccca attgctgtgc gtatctgaaa tgggtttcga tagatccagc
      121 ggcaggccaa gctggcgaat catctgctgc gccgcttgcg gcatgaaggg atacaggaga
      181 atggcgagca ggcgaagggc tcgggccgac gtatttagca cggtctgaag ttgcggttgt
      241 gtatccgctt gtttcgcaag cttccagggc gcgcttttat cgatgtattc gtcgcaggcg
      301 gtattcaatt cttcaatcat cagcagcacg tcccggaaga gcagggcatt ataaccggct
      361 tcgatcctgc cgggcagttg ccgggccagg tcttcgatac gtgcttcgag ttctggcagg
      421 accgcagggc cacgatcggg aatggtgccc gcacaggaac gctcgatcat ggtgagcgtg
      481 cggctcagga ggttgccgat gccgttggcg agcgcgctat tgatggtcgt caccatggtg
      541 ttgcgcgaga aatctccgtc ctgtccgaac ggcacttcgc gcaggaggaa gtagcggaac
      601 gcatccgcgc ggaactgatc gatcatctta ttgggatcga cgacgttgcc gcggctcttg
      661 gacattttct cgccatcgac ggtccaccag ccgtgcgcaa aaatcgagtg aggtagcggg
      721 cgctccagcg ccatcaacat cgtggaccag tagaccgcgt gggtggtgag gatgtccttg
      781 cctacgagat gggtattggc cggccagaat cgctcgagtc ccgatttgta ttccagggcg
      841 gacacgtaat tgaccagcgc atcgaaccaa acgtaggtga cgtaatcttt gtcgaacggg
      901 agttcgatgc cccaggagag gcgggatttc ggacgtgaga tcgacagatc gcccagcttt
      961 tgagtctgta gaaatcccag cacttcgttg cgccgggaga cggggcgaat gaagtcgtca
     1021 tgggtgcgga tgtgatcgat caaccgttcc tgatactggc ccatcttgaa aaaataattg
     1081 tgctcgctca gttgttcgat cggccgtttg caatcggggc acaggccacc gacgacatct
     1141 ttttccgtcc agaaccgctc atcatacgtg caataccatc ccgtgtagtc tgccttgtaa
     1201 ataagctgtt tatcgtacag ctgttgaagg tatcgttgaa ccacgtcttg atgctgcttg
     1261 tccgtggtgc ggatgaaggc gtcattggaa atattcaacc gttgccagag gtttgtgaac
     1321 tgcggcgcga ggcggtcgca atgggcttgc gggtcgatgc cggctttagc cgcggcctgc
     1381 tggaccttct gcccgtgttc atccagaccg gtcaggaaaa acacgtcgcg tccacgtaat
     1441 cgccagtagc gggcgagcac gtcggcggcg acggtcgtat atgcgtggcc gatgtgcggg
     1501 acgtcgttga cgtagtagat cggcgtcgtg atgtagaagg tgtcgcgctt gttcatatat
     1561 tgtcgaatga agatagcaag atgcctgcgg tgaaagctag cttatcgcgg ccggtcggcg
     1621 gtgtcctgag gcgtcagtaa gtgacggacc cgcagcaaga tggtttcgag cgccatctgc
     1681 acgttcaggt tccgatgggc ctgccgctcc agtttttcga gatcgttgat caggtccagc
     1741 agttcgtcga tgtcgatgcg ttcagccagc gcctgcatgc cggctttttg atccagattg
     1801 agcacatgat cggagccggc gccgacggca accaggagca catcgcgcag ccagcgcagc
     1861 agccagtcga aggcttccgg tccgcgctct tctttcgaca gggcttcggc ggcggcgagg
     1921 acggtggcat acgactggag gcctttggat gaaaaaatgg cggcgaattc tttttggctg
     1981 ttccgcgctt gtgcgaggtc gcattccagg gcccgcccca attgtccgtc gctgagcacc
     2041 gccaggaagt gcgcgtcggc gggaggcagt tcgcgcttca gaatgacggc ggcttccacc
     2101 tgggtttgcg cgggtgccgt caggcgcagg gcctggcagc gggagcggat cgtggccggc
     2161 agggcatagg gtcggctcga caccaaaatg aacaggctgt ggtcgggcgg ctcttccagt
     2221 gttttcagca aggcattggc ggcgccgatc gtcatgcgat ccgcatcgtc gatcaggcag
     2281 atcttccgat caccgatcag cgggcgatag atcatctggt gttcgatgtc gcgaatgagc
     2341 tcgatcttga tttggggatt cgcgagttct tgatccggct cgatcaccat gaagtccgga
     2401 tgggtgcgcg catccacttg gcggcaggcc cggcaggcgc cgcaggcatc cggagtctga
     2461 tcgtccggga cggtttcgca gaggagcgct tgcgccaggc ggagggcgag gaggcgtttg
     2521 ccgatgcgat cgtcgccttg gaagaggtag gcgtgcgcaa gccgattctg caggattgca
     2581 gaccggagca aggttttggc gctgtcatgg ccgatgatat cagcgaatgg catggcgagc
     2641 ctgtgacttc tgaggtgacg gccggcttgc tgccggcctg ttgtggtcta cgccccggcg
     2701 gatgtgaccg agcatcggcg tgacgacccg ggcgactgcg cgggcgaccg cctctttcga
     2761 tgcccttgcg gacaggacct tgattcgtgc ggggtgacgt ttggccagat cgagaaatcc
     2821 ggtccgaacg ctccggtgaa accggagaga ctcgcgatcc agtcggttca actcggcggc
     2881 ggaacgccgt cgcgccagtc ccgtcgaaac ggggaggtcg aacaccagtg tcaggtccgg
     2941 catgagcgca tgggtggcga ggcgattgag tcgttcgagc gtggcgatgt ctagtcctcg
     3001 cgcatagccc tgataggcaa gggtggagtc gatgaaccgg tcgcagagca cgatgctgcc
     3061 acgcgcgagt gccggttcga tgacctgggc gacatgttgc cggcgcgcgg cgaagaccag
     3121 aaacgcctcg gtttcgggcg ccaccggttc gtcggcgcga tccaggagca gcgaacggat
     3181 tttttcagcg agcggagtgc cgcccggctc ccgggtttcc accgtgtcat atccctggtt
     3241 ccggaggtgc tcgcccagga gtttggcctg ggtcgatttt ccgcacccct cggttccctc
     3301 gagggtaatg aacaggcccc ggggtggtcg gctacgtggc gtcatcattc gggtctgaaa
     3361 aactacggag caagagggcc ggatcctatt ccaagtgatt gaaaatagca agaaaaggct
     3421 tgcgacgatt ggtgaaagac cagtatcatg acgctctacc aagggccctt cagccacaca
     3481 tgttgaaagc ttcactcaaa cgttattttc tcaccggttt gctgatcatg atccccttct
     3541 gggggaccat cctgattttg aaaaccctgt tcgtcagtct ggacggcatt ctcggcgatg
     3601 ctacggctca gttggtgacc cccggctact atgtgccggg cttggggatc gtggcgctga
     3661 tcctgctcat ttttgtgacc ggtctgtttg ccgcgaattt tatcggtcgc catgtggtgc
     3721 gacagtggga gggattgctc aatcgcgtgc cggtcgtgcg cggcatctat tccactatca
     3781 agtcgatgat ggacattctg tcgtttgcgg agcgggagag ttatcgccgc gtcgtgctga
     3841 tccagtttcc caagaacggc cactattgtt ttgcgttcgt gaccggggtg acgaaggggg
     3901 aaatgcagca gctctcgccg gatccgctgg tgcacgtcta cgtaccgacc tcgcccaatc
     3961 ccacgtccgg atatttcttg ctggtgcccg agcgtgaagt gatcgccgtg gatattacgg
     4021 tggaagaggc catgaagctg attgtctcag ggggactcta cacgccgact cctccatcgg
     4081 gcggcacgtc gcagaccagc gggaagacgt gggcgcctat taagcaacca gacgcaggtg
     4141 tgcaggtcgg ttgatcgcgc ttgcactcga cggacatcgc gtcgatttct tatggtgacg
     4201 actcaatgac tctttcatcg caggacaagg ccgtgccatc ctccattccc gggcttggtg
     4261 tcatcatcat ggcggcggga ctcggcaagc gcatgaagtc ggcgctcgcc aaggtgctgc
     4321 atccggtcgc gggccggccg atggtgatgt acgtactcga tatcgcctgc ggcctggcgg
     4381 agcagggtgt ggcggtggtg gtcggtcacc aggggacgga agtcaggaag gttgttgaag
     4441 cggtcggcgg gcaagttgcc gtcgccgagc aaaccaagca attggggaca ggccacgcgg
     4501 tgttgcaggc gcgtccggtg ttcagcggtg gcgcccaccg cagaccatcg cgctacttga
     4561 tcctcaatgg cgacacgccc ttattgacgg aagcgacggt gcgggaattg ttggcgatgc
     4621 acgatgcgca gggtgctgcg gtgacgctgt tgaccgccgt gctcgacgac gcctccggct
     4681 acggacgggt cattcgccgg cgtcgcgatg aatggctgca aggcgcggcg gacaacgcgg
     4741 tgcagagcat tgtggaagac aaggacgcct ctgaggccga acgggctgtg cgcgagatca
     4801 acgtcgggac gtacgtcgtc gacggcgagt ttctctttcc ggcgctcgac aaactcgatc
     4861 cccggaatgc gcaaggcgag tattacctca ccgatattgt acagatggcg gtccagcagg
     4921 gacgcacggt gtcggctctg cgtctccgcg cgatcgacga gggactcggg atcaatagcc
     4981 gtgtgcaatt ggcggaggct gagcaggtga ttcgccagcg gattcgggag caatggctgg
     5041 aagccggtgt cacgatgcgg gatcctgcct cgacctggat cgatgctgag gtgaccatcg
     5101 gccgtgacac ggtgctgtat ccgaatgtga cgctcgaagg ccggaccgtg atcggcgaat
     5161 ccgttgtcgt gcattccggc acccggatta ctgattgcgc catcggcgac agggtagaga
     5221 ttctggatca ctgcattctg cgcgagtcgc aggtggagga ggagtgccat ctcggaccgt
     5281 tcgtgcacct gcgaccgggt gtgatcgcgc gacgcaaggc gaaggtcggc aatttcgtcg
     5341 agatgaaaaa gacggaactc ggtgagggct ctaaggcgaa ccatttgagt tatcttggcg
     5401 acgccacgat cgggtcggga gtgaatatcg gcgccggaac gatcacctgc aattacgacg
     5461 gctataagaa atttcacacg gtggtcgggg acggtgtgtt catcggcagc gacgtgcagc
     5521 tcgttgcgcc ggtgacggtc gggcaaggtt ccatcatcgc ggccggcgcc accgtgacgc
     5581 aggatgtgcc gaaggatgcg ttggtgattg cccgggttcc ccaggtgacc cgcgagggtt
     5641 gggcggccag gcggcgcgcg ttacagagcg ggcaggtccc gcctccggct ccctcggtga
     5701 agcgcgcaat ccctgtgaag gccgcaaagg ctgtgaagcg tgcgaagact gcaaaggcag
     5761 caacatccgc aacgcgtgcc gtgaagaaga agcaaccggc agttcaacgt acaaaacgga
     5821 ggtaacggcg acctatgtgt ggcatcgttg gctacgtggg aaatcaggac gcagtaccaa
     5881 ttttgttgaa cgggttgtcg aagctggaat atcgcgggta cgactcggcc ggcgtggcga
     5941 ttcagcgcgg ggagaagatc gagattcgcc gcagcgtcgg caagctgatc aatctgcaaa
     6001 agtcgctgga gcagaaggcc atcggcggga tgtgcggcat cgggcacacg cgttgggcga
     6061 cgcacggcaa gccctccgag cagaacgccc acccgcaccg ctccgagagt tgtgtcctgg
     6121 tgcacaacgg aatcatcgaa aattacgtcg agctgaagca gcgcctggtc aaggacggct
     6181 acaagtttca gtctgaaacc gacacggaag tcgtggcgca cctgatcgat acacacatga
     6241 agaaaagtaa gctgcacctg gcggatgcgg tgcgcgcgac ggcgaaagag attcgcggaa
     6301 gttatgccat cgcggtgatt tcggagcggg agcctggcat gctggtcgcg gcccggtccg
     6361 gttgtccgct ggtcatcggt cgcacggccg atgcgtcgtt cgtcggctcg gacgtgatgg
     6421 cgatgttgtc ccataccaga gacgtgacgt ttctcgaaga aggcgacgtg gtcgaagtca
     6481 cagccggagc ggtgacgttt acggatctcg acggtcgggc cgtcacgcgc aagaagacga
     6541 aggtcacgtg ggatgcctct gccgctgaaa agagcgggta cccccacttc atgctgaagg
     6601 aaattcacga acagccgcag acgattctgg ataccatccg gggccgctat tcctatgaga
     6661 gcggcgaggc ggatcttccg gatatcggat tgacgccgaa agagttcgcc gaagtcgggc
     6721 gtatttggat cgtggcctgc ggcaccagtt ggcatgccgg cctcgtgggg aagtatctgc
     6781 tcgaagagat ggttcgcacg ccggtgcagg tggatatcgg gagcgagttc cggtatcgcg
     6841 acccgctcat tgaaaagaac gacctgttca tcacgatctc gcagtcgggt gaaaccgccg
     6901 atacgctggc cgcggcgcgt gaagcgaagc aaaaaggagc gcgtgtcgtc tcgatcgtca
     6961 atgtggtggg cagcaccctc gctcgcgagt ccgacggggt gctctacacc cattgtggtc
     7021 ctgaaatcgg cgtcgcctcg acgaaagcgt tcaccagcca gttggcggcg ctctacatgc
     7081 tggccttgca tctcggccgg gtgcggggtg tactgagtgt ggcggacggg aaggcctggc
     7141 tcgatcgcct ggtcacgttg ccggcattgg tgaaacacgt gcttggccgg gaagcggaga
     7201 ttctggcgat cgcgaaacgg tattacaaga aaccggattt cttgtttctg ggtcgcggga
     7261 tcaactatcc gatcgcgctg gagggggcgc tcaagctcaa ggagatttcg tatatccatg
     7321 cggagggtta tgccgcggga gagatgaagc acggaccgat cgcgctcatc gacaaggaca
     7381 tgccggtggt ggtgttggcc cctcgggacc ggctctatga gaagaccgtc agtaacctca
     7441 tggaggtgaa agcgcgccgg gcccccgtga ttgcgttcgt ggcggaaggc gagcgcgagt
     7501 tgggcaagat tgccgacgcg gtcttcacca ttcccgatgt gcatccgttg ctgtcgccca
     7561 ttctctttac gatcccgctg cagttgctgg cctatcacat tgcggtcttg cgtggcgaag
     7621 atgtggatca gccgcggaac ctagccaaga gtgtgacggt ggaatagtcg gatgttgaac
     7681 atggcttccc actgcgttct cggtcgcccg gcctcctgcg acgtacccag agggtacgac
     7741 tcgtcgccgg gcttcctgcg gccttgtggg aagaccatgt tgaacatcct tacagaaatg
     7801 agagcatgag atggagatca cgaagcagga agtcgagaag gtggccaagt tggcgcggtt
     7861 ggcgttgacc gaggccgaga ccacggcctt ttcgcagcag ctgaatcaga tcgtggccta
     7921 tgtgcagaag ttgaagtcgt tgtccacgga gggcgtggag ccgacgtcga ccgtgccggg
     7981 gcagagtaat gtctttcgtc cggaccaggt gcaggcgtcg ctgtccaccg agcaagcgtt
     8041 gagcaatgct cccgatgcgg aggcgcagtg tttccgggtt ccgaaaatca tccaagaatc
     8101 ctaagcgatc gaaggagcat caatcgacta tgtttcgaca aatgttgcgg gcaaagatac
     8161 atcgagcgac ggtgactgag gcctgtttgg aatatgaagg cagcctcacg gtggacgaag
     8221 atctgttgga cgcggcgggc attcttccct atgaagcgat cgtctgttcc aatctcaaca
     8281 acggcgaacg gttcatgacc tacgccatga agggaaagcg gggacgaggg gagatcgtgc
     8341 tgaacgggcc gaccgcccgc aaggcggcgg tgggcgatca gattattatt ttctgctacg
     8401 agtattacag cgatgaagag atcaagcggc ataagccgaa gatcatccag gtcgatggca
     8461 aaaaccgtat cactcgtaag gctgtgaaac gctgatgtcg ctgacgttgc tccacaaact
     8521 cacgctgtcg gaattgcagc ggaagttcac ggccggggat gtcacggcga cggagatcgt
     8581 ccgcgcctac ttcctgcggg tgacgcaggt cgagccgaag gtgaacgcct atctcacgca
     8641 gtgcaaggaa gcggcccttg cgcaggcgga acgtctcgac caggccttga aggggtggcg
     8701 gaaaaccacg ccgatgatgg ccatgcccct ggcggtgaag gacaatatct gcacggaagg
     8761 cgtgcggacg acctgtgcct cccggatgct cgacaccttc gtgccgccct atgatgcgac
     8821 cgtggtcgcc aagttgcggg cgcaaaacta tctcctgctc ggcaagacca atttggatga
     8881 atttgcgatg gggtcgtcca ccgagaattc ggccttcggc gccagccgca atccctggaa
     8941 cattcagact gttcccggcg gatcgagcgg cgggtcggcg gtggctgtgg cggctgatga
     9001 atgtgtcgcg gcgctggggt ccgacaccgg cggttccatt cgccagcctg cggcgttctg
     9061 cggcgtcgtc gggttgaaac cgacctatgg ccgtgtgtct cgctatggtt tggtggcctt
     9121 cgcctcctcg ctggatcaaa tcggtccgat caccaaggat gtgacggatg cggccatctt
     9181 attgggcgcc attgcgggcc acgatccgcg cgattcgacg tccgcgaacg tgccggttcc
     9241 cgactatctc aaggcgctca aacggaaaga tctcaaacgg ctgaaggtgg gggtgcccgc
     9301 cgagtatttt gccgacgggc tcgatccgga agtggaccag gcggtgcgtg cggctatcga
     9361 aggcctgcgg gaactcgggg cggacatccg cgagatcaag ttgccgacca ccgatgcggc
     9421 cgtcgcgacc tactatgtca tcgccaccgc cgaggccagc tcaaacctgg cccgatacga
     9481 cggcgtgaag ttcggtttgc gggcagcgga gagcaaagat ctcctggaca tgtacctgaa
     9541 gaccagagcg gaaggattcg gcccggaggt caagcgccga attatgctgg ggacctatgt
     9601 gctgagcgca ggctactatg acgcgtacta cgggaaggcg caggcggtca ggacgttgat
     9661 ccggcaggaa tttgaggcgg cgttccagac ggtcgatctg atcgtcaccc ccgtgactcc
     9721 gaccaccgcg ttcaagttcg gagagaaggc ccaggatccg ttgcagatgt atttgtccga
     9781 catctacacg atttcggcga atctggccgg gctgcccgcc attgcgttgc cttgtggatt
     9841 cagcaaggcg ggattgccga tcggctttca gttgatcggt cggccgtttg aagaagagac
     9901 cctgttgcgt ggggcgcatg cctacgagca ggcaacgaac tggcgggcaa aacggccggc
     9961 gattcggtga gcctgacaag gaggccctat gattgttgat gtcgccgcga ttctggtcgc
    10021 cattgccttt gcggtgctcg tgggctacct cgtcccgctc ttgattcaaa tacgcaagat
    10081 ggtggccgaa tcggagcagc tggtgacgaa gttgaacgtc gagttgccga cgctaattac
    10141 cgaattgcgc gcgatgagtc agaatctgaa tgatgtgacc gagcaggcgc gtggcggggt
    10201 tgagcatgcg gcggtcctgc tgcatgccgt aggggagatc ggcgaatcgg tcaatcagat
    10261 acatagtgtg gtgcggggct ccggcggttc gttgttggcc aatgtggcca gtgtagtggc
    10321 ggggtttcgc gcggcaaagc aagtggtgac ggaacgtttc aaagagggag ggcatcacaa
    10381 tggcggataa tcaaggtcca tcgtcggcag cggtattgtt gggcttcctg agcggagcgg
    10441 cgttgggtgc ggtaacagcc atcttgttgg cgccgcgaac cggacaggaa tcacgcgaga
    10501 tgctgcgcgg ctatgcccgg cgcgcggagg acgggctgcg ggatttggtc ggcgaagcgg
    10561 gtgagcggtt tgaaggggca gtcgaagaag gtcgcgactt tattgaatcg aagaaaaccg
    10621 tgttgcgcga tgcgttcgat gcagggcggg aagcgatgcg tcgggagcgc gagcatttca
    10681 cgaaggggga gcagagctga gcgtggcata cgaagtcgtc atcggcgtgg aagtgcatgc
    10741 gcagctgcgc acgcagtcga aattgttttg tgcctgcggc acgaccttcg gcctcacggc
    10801 gaattcgcag acctgtccgg tctgtttggg gttgcccggg acattgccgg tcatcaacga
    10861 gaaggccgtg gagatggcgg tgcgcgccgg gttggcgatg aacggcacga tcggggtgca
    10921 gaatcgcttc gcgcgcaaaa actattttta cccggacctg ccgaaggggt atcagatttc
    10981 gcaatacgaa gcgccgattt gtgagcacgg ctggattgaa attgcggccg gcggcagccg
    11041 caagcgtgtg cgcatccggc gcgcgcattt ggaagaagac gcgggaaaga atctgcacga
    11101 ggtcgacagc gggatgagcc tcgtggatct gaatcgctcc ggaacgcccc tgttggaaat
    11161 tgtgaccgaa ccggacttga gctcttctga agaggtggtg gcatacctca aggcgctgcg
    11221 cgacctcttg atgtatctgg atgtctgtga cgggaacatg gaagagggga gtttccgctg
    11281 cgagccgaat ctgtccctgc ggcctgttgg gcagaccatg ttcgggacga aggttgagtt
    11341 gaagaacatc aactcgttca agttcgtgaa ggacgcggtt gatttcgaaa tcaagcggca
    11401 aaccaaggtg ctcaacgagg gcgggaagat ttatcaggaa accaggctct ggaatcacga
    11461 gcgcggggaa accgctgtga tgcgcagcaa ggaagaggcg catgactatc ggtattttcc
    11521 agaccctgac ttggtcccga tggagatttc gtccgaatgg atcgagcaac tgcgcgaggg
    11581 actgccggaa ctggcctcga ccaagcaaca gcgatttatc acggagtacg gcgtgcctga
    11641 gtatgatgcg gggattctga cttccagcaa agcgctggcg atgtatttcg acgcctgcgt
    11701 gaaactgtac ccgcaaccca aaacggtgag caattgggtg atgggcgaat tgctgcgtga
    11761 attgaacaac tccgggattg aagcggccgc ttctcccgta acgcctgaac ggttggtcga
    11821 gttgttgacc ctggtggatc aaggggtgat cagccttaag gtggcacgag agatttttcc
    11881 tgatgtctat gcctcgggta aggcgccggc ccggatcgtc caggaaaagg gactgacgca
    11941 ggtgtccgat gaaggggcct tggcgacgat gattgatgaa gtcttgaaga aaaatcctgc
    12001 ccaggtcggg caatttaagg aaggcaagca gcaggtgctg gggttcctcg tcggacaggt
    12061 gatgaaggcg tccggcggta aggccaatcc cgggaaagtg aatgagttgc tgaagaaagc
    12121 cttggcctga ggctggcgtc cggtggtcgc cgcgtcacgc atggcgacgc ggtgttcggt
    12181 gaagcggtta gggagacggc agtgttgatg gttcagtgtg agtacctgga ggagacacga
    12241 gcatgagcgg aatcagaaac gtgaaggcgc ggcagatcat tgattcacgg ggcaatccca
    12301 cggtggaagt cgaagtgctc ctggagagcg gtgcgcacgg tcgcgcggcg gtgccgtccg
    12361 gggcgtcgac cggtgaaaag gaagccatcg aattgcgtga cggcgacaag aagcgctgga
    12421 tggggaaggg tgtctccaag gcggtcgcga atgtgagtaa gaccatcgcg ccaaggttga
    12481 tgggcatgga ggcgttggat caggcggccg tcgatcacga aatgatcgcg ctggatggaa
    12541 cgaagaccaa ggggaaattg ggcgccaatg ccattctggg cgtgtctctg gcggtggcca
    12601 aggccgcggc caacgagacc ggtcaaccgc tctatcgcta tcttggtgga acgaacgcgc
    12661 gggtgttgcc ggtgccgctc atgaacatta tcaacggcgg cgctcatgcg gataaccggc
    12721 tcgatctgca ggagtttatg atcatgccgg tgggtgcgcc ccgcttcagt gatgcgttgc
    12781 ggatggccac ggaagtgttt catacgctga agtcgctcct aaagaaaaaa gggctcaaca
    12841 cggctgtcgg cgacgagggg ggattcgcgc ctgatttgca atcaaacgaa gaggccctcg
    12901 cgctgattat ggaagcgatt gaagccgccg ggtatcgccc cggccaggat atcgcgctgg
    12961 ctttagactg cgcggccagc gaactgtatg aaaaagggcg gtatcggttg gaggctgaaa
    13021 agaatcctga gcgttcatcc gaggaaatgg tggcctatta cggcaagctg ttggatcgtt
    13081 atccgatcct ttcggtcgaa gacggcttga gcgaattgga ttggaagggg tggaagattc
    13141 tgacggaaaa gctcggcaag cgagtgcaac tcgtgggcga cgacattttc gtcacgaacg
    13201 tcgagatctt tgccaagggg attgcggagg gcatcggaaa ttcgatcctg atcaagctca
    13261 accagatcgg cacgttgacg gagacgctgg atgccattga actcgccaag cggtccgggt
    13321 atacggccat catctcccat cgctcggggg aaacggaaga tacgacgatc gcggatgtcg
    13381 cggtggcgac gaacagcgga ttgatcaaga ccggctcgtt gtcccggacg gatcgagtcg
    13441 caaagtacaa ccaactgctt cgcattgagg atgaattggg cgcagcggcc gtctatcgag
    13501 gccgtgcagc ggtcccatcg agggcctaat cgtgatgatg atcaagccga accggggtcg
    13561 cgactggttg gattggcaac ggaagctctg ttccgccggc aagtgggtgg ggctcggagc
    13621 gctgttcctc atgatgggaa ccctgttgtt cggcgagatg gggatttcgc ggtatttgca
    13681 tttgcgtgac catgcggagc agcttgatca ggagctggcc gagcttcagc ggttgaacgg
    13741 tgaactgcgc acggatctgg atcgtgtgca atacgatccg actcgcattg aagaactggc
    13801 tcgggaacgg ttagggtatg tgcggaaagg agaaacggtg tatcaattgg ctccgattgg
    13861 agagaaggaa cggtttccca cggtgaggac accatgaagt ttggaagtgt ggccatcatc
    13921 ggacggtcga atgtcgggaa atcgacgctc ctcaatcgtc tgctgaaaga aaaaatcgcc
    13981 attgtctccg acaagccgca gaccaccagg acgcggattc tgggtgtggc gcatgttgag
    14041 ggcgctcaaa ttgtctttct cgatacgccc gggtttcacg agccgcatca cttgttgaac
    14101 cgccgcatgg tgcgcacgac actggacacc ttcgacgatg ctgatgtgct gtatgtggtg
    14161 gtcgaagcca ccgctcagcc aggccctggt gatctcgccg tcatcgaaca tgtgaagcag
    14221 gcggttgcca agcaggcgcg gccggtggtg ctagtgatca ataaagtcga tctggtgaac
    14281 aagtcgcgct tgttgccatt gatggagcag tatctgcgga ttttcccctg gacggaaatc
    14341 gttccggtct cggcggaaac cgcggacaat gtggatcgcc tgttgtcggt gacggtgtcg
    14401 ctcctggcgg aaggtgaggc gacgtttggc gacgatgtgg tcacggatca atcgatgcgc
    14461 accctcgcgg cggaactgat ccgggagaaa attctgcagc agacctacga agaggtgcct
    14521 cactcggtgg cggtcgagat cgaggagttt atcgagacca agaaattgac caagatcggc
    14581 gccgtggtgc tggtcgagaa agagtcgcag aaaggcattc tgatcggcaa acacggcgaa
    14641 cgtctcaagc tggtggggac ggcggcccgg gaagatatgg aacgcctgtt cgggacgaag
    14701 gtctttttga aggtctgggt caaagtgcgc gaatcctggc gggaagacga acaaacgctt
    14761 gcggagttag gttattaacg acctcaccat ccaaccgccc gagcctgcgc gacttcctga
    14821 tcgggagccg ctcgggaaag atgcgtctcg tgacgccggc ggcggacagg cgaagcccga
    14881 cgtgcggctg gtctccattc agcggcttct gcgccgtggc gccatcacca atctggccaa
    14941 gatgctggcg cgcatgcatc cggcggatat tgccaacgtc atcgaccatc tttcctcact
    15001 gaaagaaaaa cgcgaaatct tcgaactggt gcgcggagac gtcaagcgtg ggcaggtgct
    15061 cagcgaattg gacggcgaga gcatcacgct ggtgttgtcc gatctgctgc cttccgatgc
    15121 cgcctggttg ttgaaagacc ttggttccga cgatatcgct tacattttga gtgtgattcc
    15181 gaacgatcgc gccaaggaga tcctggcgct gatgcggacg gaggactcca ctgaaatcgc
    15241 cgacctgctg aagtatccca aaggcacggc cggcggcatc atgaccaccg agtttttcgc
    15301 gctcttcgag gatgccacgg cgcaggaagc cattcgtcgc ttgcagcagg ccaccgacgc
    15361 ggaaatggtg ttctacatct atgtaacgga caaggaggac cggcttgtcg gtgtgttatc
    15421 gctccgccaa ttactgaccg tcccaccggc gactccgtta aagaacatca tgacccggga
    15481 tctgatcagc gtggccgtgg acatggatca ggaggaggtc tcgcgccagg ttgcgagcta
    15541 caacctgctg gccattccgg tggtggagaa agacggcaaa ttggtcggca tcatcacggt
    15601 agacgacgtc gtcgatgtca tccgtgaaga agcgaccgaa gacatgttga aaatggccgg
    15661 cgccatcgaa gaggatgcga tgttcaagtc ctccagcatg gccgctgctc gtgtgcgcct
    15721 gccctggctc tttaccaatc tcgtcgggag ccttctgtcc ggcatgctgt tgtggatgtt
    15781 ccggtatacc attcaggaag tggtggccat cgtcagtttt atccccgtca tcgcggccat
    15841 ggggggcaac gtcgggttac agtcctccac gctgattatt cgagggctcg ccaccggcct
    15901 ggtggaactc accgacgtgt ggaagatttt ctttcgcgaa gcgaagattg cctttttgat
    15961 gggcattgcc tgtagtctga tgctgacggt ggtgggctgg ctgtggcatc aggtcttttt
    16021 gggcatggtg gtcggtgtgt cgctcattac ggcgttcctg gtctcgacga gtatggcgac
    16081 ggtgatgccg atcgtcttga agcggatggg cgtggatcct gccgtcgccg ccggtccatt
    16141 cgtgacgacg gccaatgaca tcaccggcat cgctatttac ctcacgctgg cgacgatctt
    16201 cctcgagcaa attcgataat gatgcgcggt gggtctcctc gtttccctgt catccttcac
    16261 gctgggtggt gagatgccgt tggtgaagac tgctgccatc gtgctgcaca gccgaaagtg
    16321 gggtgacgcc gaccggatcg tcacgttcta tacgatgcgt ataggcaagt tgcgcggggt
    16381 cgcgcgcggc gcgcgtcggt tgaagagccg tctcggcggg atgatcgagc cctttaccct
    16441 ctgccagctg gatctgtttg agaagcccgg cgattcactc taccggattt cacaggtcgc
    16501 gctggatgag ccgttcgcca aatttcgaga tgatctgacc ctcatgaccg ccgcggggcg
    16561 catggtcaac cttgtgagtg ccgtcatggc ggaaggcgat ccggatccgc gggtatttga
    16621 gatgttggag tcggggttac gcacgttgtt ggagagtcgg gacgcggcat ggacgacctt
    16681 attgttccag attcgtctgt tggggatgac ggggttccgg ccgcaaaccg agcagtgtgc
    16741 aacctgcggc caagtgcatc gaagtccgct cccgcaattt tcacccatgg ctggggggat
    16801 cgtgtgcgag cggtgcgcga gccgtcagcc gtttcgttgt acggcgttgt cccgcggaag
    16861 cgtcgcattt ctccatcaag cgttgcatat gcagccggcg ctcttgaatc gattgcatgc
    16921 ggccggccag gtgcggactg aagtcgagtc ggccatagaa agttatgtca cggtggtggc
    16981 ggggcgacga cttccgccgg tcgatttttt gaccagtgga agccctgcgt aaaagggcat
    17041 ccggtttgag tgttgcaggt gttttcggat ttctagtaag ggagcgttgc ctgttatgac
    17101 tgagacggtg cttgaaccga ccgatatgga atgggtggcg aagggtgtct tgagcatcac
    17161 gtggagtgac gggcacaagg cgctctatcc tgtccgttat ctccggcagc attgcccgtg
    17221 cgccgcctgt acggatgagt ggacgggaga gcttcggctg aagccggatg acgtgccgat
    17281 ggtgatgatg ttgcaggatg tgcagccggt gggacggtat gcgttccaat ttacatggag
    17341 tgacgggcac gatacgggga tctattccta tacgtttctt cggcggatgt gccagtgcga
    17401 tgtgtgccag ccggtgaaac ctgtggagcc gcggtccaga cggttgctct gatcagtcgg
    17461 acgcaggtgt atgacggaac gggaggtcta ctcagatgac acaagcgcga atcattgctg
    17521 tgggatggtg gtggcttgtc gctatcctct cggcgggctg ttcgagtctt ccttcgttgg
    17581 atcagcaaaa acagctggtt cagcaggggg actatcgaat ccatcagctg accccgagag
    17641 ctttcgttga aacctggggg gaaccgacct atacccatca gcagttcacg catttcttcg
    17701 ggatgcagga cgggcaactg attcctcagt cgcgcatggc gctcggagag tctccgcagg
    17761 ggtgggagac ggggcttgcc gcgggcgatg cgttattcct ggcctatgcc gatcgtggcc
    17821 agtatctggt gtttctcgat gaggcgctcg tgtaccatga ggcgatgacg cccgagaagg
    17881 ttcatgctgt cggcaagacc tggaagtacg aatctcagtt caagacgcga ctcgaattat
    17941 ccccggccat gaagtaagtg gtggcggctc ctcctatgat ggctcctcct tccaatcatc
    18001 gtcccctgaa gatctgtatg gtgtcctccg aggtcgtacc ctttgcgaag acgggcggac
    18061 tcgccgatgt ggtcggagcc ctcgcgactg agtttgccag gttggggcac gatgtctgcg
    18121 ttctgctgcc ggcctatcga caggtcgatg cgctgggcta tcaggtggtc gactatgccc
    18181 ggttggcggt gccgaccgcg caggggctgg tagaggcgcg tatccaagag cgcaccggcc
    18241 cgcattcgca tgtgacaggt tctggacgcc tgcgagtgtt gactgttcgc cacgatgcgt
    18301 tcttttcccg ctcaggtctc taccaggagg ccggtcacga ctatcccgac aatctcgagc
    18361 ggttcgcatt tttttgtcgg gccgtcatgg aattgttggt gcacttcggt gaaaaggacg
    18421 gctggcaggc ggatctgctg catgtgcatg attggcaagc cgccctgtgt gcagtctact
    18481 tgcggacgct ctatcaagcg aagtccgccc tcagcaatat ccgcagcgtg ctgaccatcc
    18541 ataatctggg atatcaggga ttatttccgg ctgaacattt ttccctcacc ggcctgcctg
    18601 cccagctgtt tacccccgct gcgcttgagt tttacgggaa actgaatctc ttaaaaggag
    18661 ggcttgtctt cgccgatcgg ctcaccacgg taagtcccac gtacagtgaa gagattcaga
    18721 cgccagaata tggttgtggg ctggaagggg tgatcagcgg gcggaaggac gtgctgcaag
    18781 gaatcgtgaa tggcattgat actgagatct ggaatcccgc caaggatccc cacctgccga
    18841 cccagtattc tctgtccgat atgtccggaa aggcgcgatc caagaaaggg cttcagcgtg
    18901 aactgaagct tcgtgccgac aaggggccgc ttgtcggggt gattgcgaga ctgactggtc
    18961 agaagggtat cgatctcgtg atcgatatta ttccggaact gatggaactc gatgtgcagg
    19021 tcgtgattct cgggaccgga gatgtgaagt atgagcagct ggtgcgtgag ttggcggaac
    19081 ggtatcccgg gcgactcgcg gtgcgcaacg tctttgacga agggctggcc catcggattg
    19141 aagccggcgc ggatatgttt ctcatgccgt cccgttatga gccttgcggt ctcagtcagt
    19201 tatacagcct tcgctatggt actgttccta tcgtgaggaa gaccggaggg ttggccgaca
    19261 ctgtggtcaa ctatacgccg agcgctttca agggatcgcg tgtcaccggt ttcagcttta
    19321 cggataccag cgcggactcg cttttgacct gtctcttgct tgcattgtcg atctaccgga
    19381 aaaaggcaga ttggcaccgc atcgtcaggg cgggcatgga gcaagatttg tcgtgggctc
    19441 gatcagccga gatctatctg cggttattcc aggagctgct tgaagggaaa gagccgcagc
    19501 ggaagtaggg ctcaccagcg cgaagcgtag agactaccgg agagcggctt ggagtgtgag
    19561 gtcgaggtac gacagccgtt ctcgagcttg cgaggcgtag taagaatagt cggattgtgt
    19621 ctggttttct agcaccagcc tgaactcatt tcttgccaga tcatgttctc cgactgacac
    19681 ggccagattc ccagcatgta ggctacaggc agcggccatg gcatgaacgt ccaccgccag
    19741 tctcgaggcc ggctgggtca ccatgctctt cagcttggtc gggaggaatt tttcgagtgg
    19801 tgagcggaga gcttgggcct ggctgacctc gcgtaatttg tgcgaatcga ggagggccga
    19861 ttggctttgg ttcgaggcca agcaatgtgt gtaggtgctc cacacatcca taaatcggac
    19921 attatcgtat cgaaccgagg tggtcgcagg gttggattgg caaccaacga ccgacagggc
    19981 agcgaccaga atggcgagga tgccgactct gatgtctcgt ctatcgctca tgtgccgcct
    20041 cctccaatcc gtgtctcttt gtcgagacag caaggtattg agcaagtgtc gggccacggt
    20101 ttgcaatcgt aacaaattga aataattgac cttaaattcg tattttggag tcgttcctgt
    20161 ctcaataata tgtcactgtg ttgaatagaa cacggaagat gagagccgga cagggatctt
    20221 gaatgactaa acggtggtcg catcgtgcct tccctggcgg tcgacctagc cgtcgagatt
    20281 gcgcggatcg ctttgtggcg tgatgggtag tgtctggtgt agctgagtat tcaggactgg
    20341 ttggcgttat cctgcgatgt tctcggtggt acctcgcaaa ttcggtgata gagaacttct
    20401 ccatgaccga gtccgtgctc ggccatcgct cgcaggacga cggatgcgag atggtattcc
    20461 tgtgacgatg cggtggcggc agtgcccgtc actgggctac tattctgcaa ctgggcggcg
    20521 gatgtagcga agtggggcat cagtggtagc taaagatgaa ggagcccatt gttgggtgaa
    20581 tgcccctcac atggttcggg tagagtgctg gcggttgtgg ctctagcgaa ttttgtgcgt
    20641 ggctgatatt gtcgggaact cgtggtgtgc atgtcctctg cagtgtgtct ggtcatgtaa
    20701 cccggcggca cgcgttgctt caagcagctg gttccgcaga tggggcggtt gttgccatcc
    20761 gactgcctga ccgttttgac atctatccca gcagtcaaac cgtatcgagt gccatccttt
    20821 tggtgaaagg ttcctccgct catgaatgcg agccaagcgt tgaccaagac acgtcaattc
    20881 agaaacctgc tcctctctga acaactgcag ttcatctgcg aagcccacaa cggactcagc
    20941 gcgaaaatcg tccaagaggc cgggttcccc ggtatttggg ccagcgggct atctatatcg
    21001 gctcagttcg gcgtacgtga caacaatgag gccagctgga cccaggtgct tgaagacttg
    21061 gaattcatgt ccgatgccac ccgcattccg atcctccttg acggtgacac ggggtacggc
    21121 aatttcaaca acatgcagcg gctcattcgc aaactcgaac agcgcaacat cgccgcggtg
    21181 tgcatcgagg ataagctgtt ccccaagacc aacagttttc tcaagggcga tgcgcagccg
    21241 ctcgccgata tgcaggaatt ttgcggcaaa attaaagctg ggaaagatgc gcagaccgat
    21301 ccggattttt gcctgattgc cagggttgag gcctttatct gcgggtgggg gctggcggag
    21361 gcgctgcggc gtgcggaggc ctatcatcag gcgggcgcgg acggcattct cattcatagc
    21421 gcgctctcgg tgccggatga gatcctcgcg ttcaagcggg aatggggcaa tcgttgtcca
    21481 gtggtgattg tgcccacgaa atactatgcg acgccgacgg atgtgtttcg gcagcatggt
    21541 ttttcgatgg tgatctgggc aaatcatatg ctgcgtgcgg ccgtggccag catgcagaaa
    21601 accgcgcgcg cgttgaagga gagtgagcat ctcctctcca ttgaggataa ggtcgcgccg
    21661 gtctccgaaa tatttcggct gcagaatgcc gcggagttgc tggaggcgga agaacgttat
    21721 ctgccccgag gtgccgaggg cacctcagcg gtagtattgg ccgcttcccg gggagaggaa
    21781 ttgggggagc tgaccgagga ccaacccaag accatggtga aagtgcaggg cgttcccatc
    21841 ctgagccaca tcgtcgatgc gtataatgcc gtcgggatta aagatatttt agtcgtccgt
    21901 ggctataaga aagaggcggt gaacctcccc aacttgacgt atatcgacaa tctcgaattt
    21961 gctgagaccg gtgaattggc ttccttgtcc caggctttgc aatccagaaa agggcgtttc
    22021 cagccgacga tcgtttccta tggagatgta ttgttcaata aatatattcc gcaggcgttg
    22081 tgccaggagc aggatgactg cgtgattttc gtcgacagca actggaggga tcaaagcagt
    22141 tatgcccgtt tgggcggttt tgccgaatgc tccgttccga attcgcggaa agccttcaat
    22201 gccaaaatcc atctcaagca attggggaag acccttcccg aggagtcgat ccatggagtc
    22261 tggatggggt ttctaaagct ctcttccagt gcggccagtc aggtcaatga tctcctgttg
    22321 ggcatgcttg ccgatccctc gaatcgaagg gccggtattc cgcaattgct gcaggaactt
    22381 ctgaaacggg acgtcccggt tcgtgtgttg tatacggtcg gtcattggtt ggatgtcaac
    22441 agtcttgagg atgttgtcca ggctgggaat ttctgagttt caatccaagt aatattcagt
    22501 taggtggtga gggtcggagg aaaagtcctg tccgcagtga aacgcaggtg aggtctggct
    22561 atacgaggca taggcctcag gagatacgac ggcatgattg atccacggga gtttattgcg
    22621 caccttcagg ccagtggggt ggagtttttt acaggggtac ctgattctct gttgaaggag
    22681 ttctgtgcct gtcttgagca tgttcctcgt gccggtcacc acatcatcag tgctaacgaa
    22741 gggggggctg tggcgctggc gctcgggtac catctcgcaa cacggaaggt tccgctggtc
    22801 tatctgcaga attccgggtt gggaaacatc atcaatcctc ttttgtcgtt ggccgacccc
    22861 gaagtgtatt cggttcctct cttgcttgtg atcgggtggc gaggagagcc aggtgtccat
    22921 gatgaaccgc aacacaagaa gcaggggcgg gtgatgctct ccatgctcga agcgatggaa
    22981 atcccccatt ccattttggg gcctgagttg gttgatccga aggcagcgct ggcggatgcc
    23041 ctggctcatg ttcgaaagat gagcgcaccg tatgcactcg tcattaaaaa agggactttt
    23101 caatcgttcg ctgcccccaa agtagagaaa agagattttc cgctttctcg tgaagaagcg
    23161 attcagcagg tgatcgaatc gttgggcgaa cgcgatgtgc tcatttctac gaccggcatg
    23221 ccatctcgtg aagtatatga gtataggcgc aagcgggggg aaggccatca gcgggatttc
    23281 ttaaccgtgg gagggatggg gcacgcttcc caaattgccc tgggaattgc gctccaaaaa
    23341 ccgggacgtc ccgtctattg tcttgatgga gatggcgctc tgcttatgca catgggggct
    23401 cttgctatca ctggcgctct caaaccccgc aatttcaagc atatcattgt gaacaatggg
    23461 gctcacgatt cggtaggcgg gcagccgact gtggcctttg atgtcgatgt tcctggcatt
    23521 gcccatgcta gcggctacga atcggtattt tgtgctcaaa ctaagcagga acttcagtcc
    23581 tgtctggcag agttacagcg ttcaagtggt ccgagcttgt tggaggtccg agtacgctgt
    23641 ggtgccagga aggatatcgg gcgcccggta acaactccaa gtcagaacaa gaatgcgttc
    23701 atggattttg tcgagaattg aggcgtgagg tggttcgctc gagcgttttg tgcaggatgg
    23761 tagtttgggc gaagctcagt ctagtaagtg agcgtcaggt ctgtggctgg ctccgattga
    23821 aatccataat cttattctcg caggatgaca actagaatgc taagatcgga acacttggac
    23881 tagaactcct gtccgagtcg ttgaatttgt ctttcgcata atgtgtttga tgtgttcctt
    23941 cactgtctgc tcggtgatct tcagcgcgtt ggcgatttcc ttattggtcc atcccttcgc
    24001 cagattttcg accactgatt gttctctgtt tgtgagctga aaccgttcct tggcttgatc
    24061 ggtattaagt tgctgccggc ggccaagctc ttctaatgtg acgacaaggc gggcatactg
    24121 gatgccacct cgatcgggta agccaaatcc tcgaaggagg acgggctgat ttgggtttcc
    24181 tgcgattcgt ttgacctcaa attgttccca gtcctttgcc tcggtgcgaa cttggagtgc
    24241 tttcagaact tcggtgcaga gttcggtcaa ggcagcggga agcaccccct gggcggactt
    24301 tgcaggtgcg ccaccgtttt cagccatatt gatgagcttg gacagctcag cggcttgtcg
    24361 attcatatgc agcagttgca tggaggagga taaaacgacg attccggcac ctgcacgttg
    24421 gtcagccagg ttatctgcag gttcctgaga gtgaacagcg ttttgagcca cggttatact
    24481 ccgtatggtc cttaatctct ctagacaatc aggctatttc tgataaccta aagcatgtgt
    24541 ctgttactag taatgctaca taatactttc gaggtagtca aattatactt gtgggggagt
    24601 tatccgtacg tcattggtat tgttcgttgg cgctcgtact tctatactgc aaaagaagag
    24661 gcaattaccg tttgttaggg tatctaacat gagatgaact gtaaatgact tcggtagccg
    24721 ttgttcaatg ggtgtaccgg atctgtccat caaccgtaaa ttgcttagtt attaatggta
    24781 taggccatgg tggattccag ccaggcgctt tgtaaagttt tgtgcggtaa tgaggttcct
    24841 ggagatgcgt gatctaagca gtcaaaatgg gcctgtagcc caggacggat gggactggcc
    24901 cacaattgcg gccagcgaga agcacgccga gcggctagcg ctccttcgtc caattcctta
    24961 tgaaatgtcc ctaccgaatc atgaaagtgg tcaaaacgga cccagcgtgt tccacggacg
    25021 ggcgctctca ttgaacataa gcagtggtgg gatgttgatc ctcatggctc aggcgccact
    25081 gcttgatcag gtcatcaagg tacatgttcc gacccctgtt gcgctggccg agaccccaac
    25141 acttgcggaa gtgcgctgga ttagaaaagt ccccttttca gcgtcgggaa gtgaggcatc
    25201 ctttttcgtg ggtttgaagt ttttgttctc ggcgggaagg tagatgctag gcagttgttg
    25261 caagtggcca gctggaattt cttcaggaga ataagtcatg aatggtaagg ccttattgag
    25321 cgggattatc cttgtgggaa atgtgatcgg tgtggcgcat gccggacaat ttgaccaggt
    25381 gcccaagccg acgttagtag cgggatcgtc tccaggggca agttcgtctg gggtccgcca
    25441 cggaggcgaa ggggcagaaa aatcatccct tacggtgacc ccggattata ttatgggccc
    25501 agaagatgtg ttagagatta cagtctggaa aaatgctgat ctttccaagc aagttcaagt
    25561 tcgacctgat ggaaggattt cacttccgct cattggcgat gtctcagcag tgggacgaac
    25621 tgcggctcag ctgaccgaag agatttctgc tcgactgaag tcttacatgg aaaacccgac
    25681 agtctcgatc ttggttcgag aggtaaatag ttatcaaatt tatgttcttg gtgaggtgaa
    25741 tgcacctggt aaataccctt taaaaagcaa aacaaccttg ctgcaagcga ttacgatcgc
    25801 gcatggtttt acgcaagtgg ctgcgcgcaa caagattgtg gtatttcgat tcgggaaaga
    25861 cggcgagggg ctgaacaaga ttaaggcgag ttacgacgat attgttctga gagatggatc
    25921 cgatcaaaac atagagctaa agccaggaga tcagattgtc gtgccttctg agacgatggt
    25981 ggtgttgccg tcaaggtaga gcctcgtcgg tcgaatttac ggatgacatt tcgctgaagc
    26041 gagccaaagg agtggggaaa ttgaaaactt tcggttgcag atggtttcga gggggggtat
    26101 gtattgtttt cagcctgctt ctacctctcc caggatgctg gatcgggaat gaacaaatcg
    26161 actttaatgt cacctatatc ccaccgaatg agttcttgtt gggaccggag gatgttcttg
    26221 ttgtaaatat ttggcgaaat caagagctgt cccgagaagt cattattcgt cctgatggga
    26281 aaatctccat gcctttaatt ggcgatgttc aggccgcagg aatgacatcc aatgttttgg
    26341 cgaagagaat cgcagatggg ttggctgagt ttatgtccaa tccgacagtg tcggtgcaag
    26401 tcaaagaggt caatagttat tatgtgtacg ttctcggcga agtatcaaag ccgggtaagg
    26461 tgccgctaaa gtcgtatgca acggttttac aaggcatctc gttggccgga ggtttcacaa
    26521 cgtttgcctc gagaaataaa atccatgtgc ttcgggttgt tcccaatggt caagggcagc
    26581 cgaaacaggt tcaaattcct gttccctatg aagacattat tcaaggtaaa aactcggagg
    26641 gaaatttctt cctcaaggcg ggtgatgtca ttgttgtgcc gtgaccagtg cttgaagata
    26701 ggttggttgt gttacggcgt ttttgccctg atgtcgtgtt ggactatatc cgactctgcc
    26761 gcgcaacaga tacgcgtggt gccgtcaatc tcggttatcg agcagtacga cagtaacgta
    26821 tttttcacac cgaagtcgtt attggcacca ggtacaaaag tcgatgattt tatcactgta
    26881 gttactcctc agctaaattt tatgcagagt aactcactgg tgaaaacaaa tctgtcagtt
    26941 ggcgcggttg ttcagaaatt cgtaaataac tcagccctgg acaacgtcgg attcaatgcg
    27001 agtacgggaa ttgatctctc ccaagctgtg aataggattc tgccacgaat gcgaggggct
    27061 cggatctttg gaacatacat gtatacccct tcggcccctg cattcggtgc tggcagtctc
    27121 gggggtgggg ctggaatggg agggggaggt ttcggaggag gagggtttgg tgccggaggg
    27181 atcgggattg cagggccgat tgactcggga ctgctcactc agcgaattag aaccaccatg
    27241 ttcaacgctg ggtttgcaaa ctcttactcc ctttcgccga ctacagattt tcaaactacc
    27301 tacacctatt ctcagcttag tttcggtggg tcattcaccc catctacgac agtgtccggg
    27361 caagcccaga acacggtgtt cgataccacg acccattcca tttcagcagg gccgacgtca
    27421 cagatttccg ctatcgacac cttgaccgtg aagtatacgt ttatgcaaat gtctcaagcc
    27481 caatttggcg actatgcgac gcatggtggg acacttggct gggggagagc gtggaccaag
    27541 gagttgagtt cctcgcttaa cgggggcctg actcttgttg agcctattcc ggacctctct
    27601 acttccggag ggcagcgaag aatccccgcc acaatgtttc cgactggggg ttttagcgta
    27661 acgtatgcct caggctcttc tttccttagg aagttaggca gtgaaattca agaggcgact
    27721 ggaacgagtg gtgccggttc tgtaggaggt ggaggttttc ttccacttct tgctggaatg
    27781 aatatgcccg gtggaattgc ggctcccggg tcttatcgag tcacccttat gtataacctc
    27841 ggcgtgtttc caagctttgt tcaatctgcc gggcctatat atacgcatac aatcttattg
    27901 ggtggtaccg ctggaatcac tgataggctg agcgctcagg cgcttttcaa ctatgcccga
    27961 agcagttaca catctgaggc cattggtacg acgttcagca cctatggaac gactgtatcc
    28021 ctcaattacc tcatcacacc catgttgact gccagactaa gtcaccagtg gctcaagttt
    28081 gataatcaaa cgagtggtgt gagtgctgcc gatttcggtt ttgcaaaaca agtagttctg
    28141 cttggtttta catacgcgta ttcaccacga ggagattttt tccggtctgg tgccttctgg
    28201 gaaggtccct ccggtgcctc ctcgtctgct gagtcaggag tgagtaaatc tggatcagga
    28261 ggagtggata caaagaaatg aacacgcgca cattgtctcc agaggattat tggcgagcgg
    28321 ttgtcagcag aaagtggttt gttatttctg caatcttggt ctcgctaagt attgctggtg
    28381 tggtatgtgc gctcatgccg aagatttatc agtcagcgac caaaatgtgg tttgagggtg
    28441 cgaagatcga agagtccatt gtgagcgggc ctactccagc tggaagcggc tattccccta
    28501 cactggatga tcgcgtcatg gaagtgcgac agtttgtgat ggggagaaaa actcttgggc
    28561 aaattgcggg agagtttggg ctttttggat acgaaaaaga tcatccggat gctccagaat
    28621 cagagaatgc catcagagcc atgcggggat cgattaaggt tgagcccacc aaggacaagc
    28681 tatttatcac tctttcattc tctaatgaag atccgatcat cgcgagagat gtgacatcaa
    28741 gacttagtga tctgttcatt gaggaaacct tgaaggatcg ggagcgaggt gttgaggcgg
    28801 cggaggattt tcttggactt gagttaaaac atgcaaaagc tgagctcgag ataaaggaaa
    28861 aaattatttc agagttcaag caacaacatc tcggagaact tccgcagcaa atagatgcca
    28921 atcttcgcaa gctggatcga cttcaggatg acatgcggtc gcaaagtgaa caggcccaga
    28981 atctggccaa tcgattggcc caaatcgaca agtcaattaa agattacgag gagactggag
    29041 aaatcagtag tgattcgccg gtcggcggta ccagcaagcg caataaggat ccacgccttg
    29101 ggagaattaa ggagttagag cggcgcttgg tggagctgtc ctcgatatat aaggagacct
    29161 atccagatct ggtacaagtt aaggaggaga ttaagaagct ccgagaaatg acttcagctc
    29221 aatatcgcga tttgttgcct gatagcgagg gcgttgaaga accggctgtt gggaaaaagt
    29281 ctaagcgcaa ggcaattgat ccataccatg ctgaacttct caagcagcgt gaggagattg
    29341 ttcttgagtt ggatgcggta aaacgccacc aggcacatat ttctagtgaa atttctcagt
    29401 atgaacggag ggtggaacat accccagagc gggaacagaa actaaagaca cttgaacggg
    29461 actatgaaaa ccttcaaaag aattatcaat cgcttctgga caagaaactc agtgccggta
    29521 tgcagaagag tttggcccaa ggacgcaagg gagccaagtt tagcatcgta gatccggcct
    29581 ataccccagt cgttcctgtt gtgcccaaca ttccgttgat tatggcggcg ggacttgtcc
    29641 tgggctgcgc gttaggtttt ggcggtgccg tcggtcttga gcttatggga cgaggattcc
    29701 ggtctgcgga agaggtcgag ctgactttgg gccttccggt gattgcgtca attccactgt
    29761 acgaaagtgc gttcggcgga tcaatgcaaa ccgtgcgggc actttctggc aaaagccgaa
    29821 atgctgcggt gttgttaccg cgttatggaa aacagggtga aagtctggag atttctggag
    29881 gccttcctgt tgcatctgtc agcaagtcca gaggtgccaa tgggaaacta aatgacccaa
    29941 ctcctggtct ggagttggtc tcaatgtggc gaccgctttc atttgtggcg gagcaatatc
    30001 gtgttgcggc cacccggctt gagttgatga caggggatcg aaagagtacg gcgatagtgg
    30061 tgaccagtgc tgtcatggga gagggaaagt cctctacggc ccttaatttg ggctacgtgc
    30121 tggcgaaaga ccttgatcgt agaaccatag tgatcgattg cgatctcaag cggcctatgc
    30181 aacatatcta tgcaggagtc tggcagcaac cgggcctggc tgaagttctg cgtggcacca
    30241 aggtggttga agattgcttg cagagacttg gggaggcggg cccatggatt cttaccgcag
    30301 gggcggtcgg agataacccg ttggccttat cgaaaatgca tcagttggct gatctgattg
    30361 ctgaactgaa agaaaaattt gattacgtga tcattgatgc tccgccggtg ttgccacttg
    30421 ctgacatgca ggtgttggca agcatgggag atcttctcgc ctatgtggtg aaggcgagca
    30481 tgactggtcg cgatgttgta caaaaggcat tgagggtcat cggtgatacc gccaatgtgg
    30541 ggattatctt gaatggctta gacgcacata ccactcccta ctatatgcaa caggagtatt
    30601 accgcgaagc tcaccatgaa cagctcaagt aagtccgaac aggagatagg cctagagcct
    30661 gtggtcactc aaccaccact ggtttcagtg tcggttagct ttcctaaatt cacgcggcga
    30721 gtgttgattc taggtgttgg gcctcttgct cgagatctct gtcagaccct tctgtctaaa
    30781 cgaaccggtt ttaccgaggt agtggggttt cttgataagg atgcgagtag ggttggtgag
    30841 cggttggtaa atccgagcat catcggtacc tacgatcaac tatttgagat tgcggagcga
    30901 tatcaagttc ataccgtggc cgtgtgtttg gaagatcgtc gcgcggttct gcccgttcaa
    30961 acgctattgg acatgaaggc catggggcgt gatgttgttg acggccacta tctgtatgag
    31021 gaagaatcgg gccgcctttc gattgaccat ctcaagccga gtgcactcat tttttcgact
    31081 gggttccgtc gtcgattagt cacgatgctc ttgaagcggt gcctggatgt tttggtggca
    31141 atcgtaggaa tagtcgcact ggtgccgctg gtattgcttc tctctatcct tatcaagcta
    31201 gactcatccg gtcctgtttt ctaccggcaa atgcgagttg ggcttcgcgg ccgcccttac
    31261 atgatctgga agttcaggtc catgcgacag gatgctgaac aaagcggggc gagatgggcc
    31321 acaagtgaag atcctagggt atccagagtt ggtcgatgga ttcggaagtg gaggttagat
    31381 gaactgcctc aactgatcaa tgtgatgaga ggcgagatga gtttggttgg cccaagacca
    31441 gagcggcccg tgtttgtgca ggaactcaga aacaccatac cttattacga tctgaggcat
    31501 accgttcgtc caggaatcac agggtgggca caaacccgtt ttcggtatgg tgcatcgaag
    31561 gaagactcgc acgtcaagct tcaatatgac ctcttctatg tgaagaattt gtcactggcg
    31621 ttggatttgc gaattgttgt tcatacagtg aaagtaatgt tgatgggtga gggggcacgg
    31681 tagggggcgc aaatgcctga tgcaattcct agacattgtt tgtcatttga cgtcgaggag
    31741 cattttcagg tttcggcatt tgaaaccccc atgcgaagaa ggcactgggg gcaattcgag
    31801 agtcgcgtcg aagacaatac tgagagactg cttggattgc tggaaaatag aggcgttcgc
    31861 gcaacctttt ttattctagg gtgggtagca gagcggtatc cttcgctagt tcgccgtatt
    31921 gcatccggcg gacacgaagt ggcttcacat gggtatgccc atgaactcat aacaagccaa
    31981 actcctagta cttttcgcga ggatattcgt aaggccaagg gtatcttaga gaacattctt
    32041 tctcagccta tgcttggcta tcgcgcaccg agcttttcca tcaccaaaga tacgatgtgg
    32101 gcgactcaga ttctggttga agagggctat gtctacgatt ccagtatttt cccggtactc
    32161 catgatcgct acggtgtgcc ctctgcaaac ccggaggcgc atcaactgtt gaccgcatca
    32221 ggtctcctat gggaggtgcc cccctcaacg gttaagtgct tgggggtgcg gctacccgtt
    32281 gctgggggtg ggtattttcg gttgtatccg tatgcactgt tgcgtgcgct ccttcgcaag
    32341 ttggaagccg agggggcttc actagtgatg tatatgcatc cgtgggaatt cgacccggat
    32401 cagccaagaa tggagggttc attggtgtcg cgaatgcgac actacttgaa tcttgacaag
    32461 accgaaatcc gaatgcggga actcttgcaa gatttctctt ttgctccaat tcgagaagtc
    32521 tttccccaaa ttgaacaaat gcacagtgcg cggctggttt ctcaggtttc gggagtgtaa
    32581 gtgcattctc ggaaaagtcc atcagcggca ttacccagtt aatatccttc tcagggcaat
    32641 ttacccagac ttcactgacg ctttcttgct cgcgattaga ttgttcctac ttatatcgac
    32701 aaaactcaaa ctgtgctaac gttcagtaat cgcatcgtgg atcgacttcc cgcttattgg
    32761 ggcgcattgg gagagtggta tcagcagctc ccgtcccaag tcgtttctct cacatgcgta
    32821 agcgcgggcg ctgatagttc aacagaatgg ttctccgcct ctcgagaagc gtatttgctt
    32881 gcctccattg agtgtgcact attcttcgat cgtaaacagt gcatttgatt aggatagaaa
    32941 tcatttcctg ccggctgtgc tactcttgtg gtgtggaccc gtcagcattc gttgacactc
    33001 tctgcttagg cagacccagg aaaagaagtg gattccggtc atttgctcga gaatgagtgg
    33061 agaggtagtt gcgccgaaat tttcgtctcg ggtggcggaa tccaatagct aggtatcacg
    33121 atggaatcta ctgtagagca agaaagcatg gccgtgctgg taatagaacc ccgcacgggt
    33181 tatgtccagg tgggatggcg tgaactgtgg gctgcacggg aattgttgta ctttctcgca
    33241 tggcgggatc tcaaaacccg ctatgctcag accgctattg gggctggctg ggccctcatg
    33301 cagcctttgc tcagcacgct gatcttcacc cttgtattca gctacttggc aaaagtgcct
    33361 tcggatggac tgccttatcc actcttcgca ttcgctgccc ttctgccatg gtccctgttt
    33421 gcgaggagcc tggaacgcag cacattgagc gtggtgaccg aaggcggcct gattaagaaa
    33481 gtctactttc cacggctgat cattccaatc gccgccacct tcattaatct cgttgatttt
    33541 actgtcgggc tgctgattct tataggaatg atgacgtggt atcaggtgct tccccagtgg
    33601 acggttctgt ttctacctct gttcgttatc gtcgcgctgc ttactgcatt gtcggtaagt
    33661 ctgtggctgt cggctctcaa tgtgaaatat cgcgatgtgg cttcggtcgt cccattgatg
    33721 acgcagttgt ggatgttcgc ctctccggta ctctacccgg catctctggt acccgagtcg
    33781 ctccggtggt actacggttt aaatcctatg gcaggtgtga ttgagggatt tcggtgggcg
    33841 ctactaggca agtcggctcc tgactggact atggtggcgg tgagtttggg tgttgtgggc
    33901 ttcttgctga tcgggggtgt catgtttttt cggagggtgg agcgaacctt cgccgatctg
    33961 atttagatga gcaacactgc tgtcagcgtt accggtctct ccaagcgcta tcgcctgggg
    34021 acgacgcatg ctcaagactg ttctctggcc ggagccttga cgcgtgggct ccgccgcctc
    34081 atcggtgggc agccggcacc cccccagacc catgacaccc tgtgggcgct gcgcgacgtg
    34141 tcgtttgaaa tcaaaaaagg cgaagtcttc ggagtcatcg gcaccaacgg ctcgggcaag
    34201 agcacgctcc tgaagatcct ctcgcgggtg acggaaccca ccaaaggccg tgcgctcatc
    34261 cacggccgat tttgtggcct cttggaagtg ggcacgggct ttcatccgga actgaccggt
    34321 cgcgataacg tcttcatgag cggcgcgatt ctcggcatga agcggcagga aatcgcccgc
    34381 aagttcgacg agatcgtggc gtttgccgaa gtggagcagt tcatcgacac gccggtgaaa
    34441 cattattcca gcggcatgta tgtccggtta gggttctccg tgctggccca tatggatccg
    34501 gacattttga tcgtcgatga ggtgttggcg gtcggggacg tccggtttca aaagaagtgc
    34561 atggggaaga tggaagatgt ggggcagcat gggcggacgg tgatcctcgt ctcccacgac
    34621 atgccggcca tcacgcggat gtgttcccgc gcgattctgc tgaacaaggg ggagatcgtg
    34681 caggcagggc cggcgcatga agtggtgaat cactacctcc atgccgggca catccagcct
    34741 gcggtcgaat ggctggatcc aacccaagct ccgggcaatg acgtcgctcg cctccgtgcc
    34801 ttacgggtca gatccgaagc gggacaagtt acggatgtca tcgatattcg aaagcctatg
    34861 cgagtagaaa tcgagtatga catcctcaag ccagggcaga tggttgttgc gcaatttggt
    34921 ttcaataccg aagaagacgt gatcgccttc atcgtcaacg accgggaccc agtatggtac
    34981 agacgtcctc gtccagtggg tcgatatatc agtaccgcct ggattcctgg gaactttctg
    35041 tcagagggaa caatggtagt agggggaggg attttttcgg aagatccttg gcatgaacat
    35101 tgtgatgtgc cgcgagctgt gggttttcga gttgtggatc ccgggactag ggatacggca
    35161 cgaggtgacg tgacaggacg gtgggagggg gttgtaaggc ccttgcttcg atggacaaca
    35221 gagcatagct cgctataacg agggaaccag tagtcagtac tggtatggtt gttcctcgct
    35281 gggttgagag gatggcatca tgacaatcga tagcgttgtg ctgttggttt ttctgtgact
    35341 ggctaataag ccagcaaata ggttcgtggg agtaaccctg ttggcgcacg aacaggattt
    35401 cggcgtgaat agggagtagt cgtctgtgca atgcctgaga tcgagataag gctcacgttt
    35461 cacaactcgt tgggctgtgg ggatgatcct tgtaggagtg aatgatgcag agacgatggg
    35521 aggacttaac ggggaagctc agctgcacct tcttgacgag cagaatgcta tttgaggagg
    35581 tctttcagct taaagatgtt atggtaaaga gcgatggcaa tgccttacct gccatggtga
    35641 gtttgtcgtg cttgtgggta cagaattggg tcgaggcggc aaactctgtg ggtacgacta
    35701 tgagtttggt gattgatgag ctttcgatga tgttctgcga atttaagcct tgtcctggct
    35761 gcaggtaggc atatgctggg aaggcgaagg aagtgtactc gatgctctga cgtaggtatc
    35821 catgtcagta cggatggcga caatgattga ttgctggagg tagggacatg aaagcggtga
    35881 ttttagctgg gggattggga actcgattgt ccgaggaaac cgtgcttcgt ccaaagccga
    35941 tggttgaaat cggcggcaag ccaatccttt ggcatatcat gcaaattcat gcggtgtatg
    36001 gggtgaccga gtttatcatc gcccttggct acaaagggga aatgatcaaa gagtacttcc
    36061 tgaatttctt tgccatcaac aatgacttgt cggtggatct ttcaactgga aaaacgacaa
    36121 tccatgatgg aaaccagcca aagtggaccg ttcatctggc cgatacagga cagacaacgc
    36181 agacgggtgg acgggtaagg cgtttgaaga aatggctaga aaacgatgaa acattcatgt
    36241 tgacgtacgg agacggcgtg gcagatctga acatcagcaa gttgctggag tttcatcgtt
    36301 cccatggaaa aattgcaacc atgacctccg tccggtcccc agcgagattt ggtcggattg
    36361 ggtttgacgg cgaccgagtg actgagtttt ttgagaagcc tgagtctggg gaagggtgga
    36421 tcaacggcgg attttttgtc ctcaacacaa aagccttgga ctacattgat ggtgatcata
    36481 cggcttggga gcgtgagcct gtcgaacgcc tcgctcacgc cggcgagatg gtagggtata
    36541 aacactacgg attctggtcg tgcatggata ccctaaaaga aaaaagttac ctggaggagt
    36601 tgtggaattc tggcaaggct ccctggaagt tatggtgacg agcactgaat ataggaattc
    36661 tgaaatgcat aaagtcgcgg acttttagcg gcatcgtgca ctggcagaga ggagcaaatt
    36721 tcaatgaccg ctgatacatc tttgttcgca caggcgcagg catggttgga tgaagattgg
    36781 gcgcgaatgg agaagacgct gggccctcgg ttggaggcgt ttcgcgggaa gcatattctt
    36841 gttacggggg cagcgggatt tctcgggttt ggattcctcc attttttttc gtatctcaat
    36901 aggaacctct taaagactgg gcgtctgtcc attgttgccg ccgacaatta catccggggt
    36961 tgcccacgct ggctgactga gttagtcgct gcaagccccg acatacagct gctccgacaa
    37021 gatattacca agccctggac aggaccagcg ggcgatcgtt ttgatttcat tattcacggc
    37081 gcatccatag ccagtccgaa agtgtatcga caatacccgt tggaaacttt ggatacgaat
    37141 atctccggct tacggcatat gctcgagctt gccaagagtc atcgggctga aagtatcctc
    37201 tatttcagct caagtgagat ttatggtgat ccgccggctc atgaaattcc aacgaacgag
    37261 gcctatcgtg gcaatgtgtc atgtatcggt cctcgggcgt gttatgacga atcgaagcgt
    37321 ttgggcgaga cgctctgcta tttgtaccat cagcaacatg gtgtgcgcgc gaagatcgtc
    37381 cgaccgttca acaactttgg ccctggattg cgtctggccg acggccgcgt gttgcctgat
    37441 ttttgtcgcg atatcctggc cgatcgcgac atcgtccttc gctcggatgg gagtccgaca
    37501 cggacgtttt gctatgtttc ggatgcactg accggatatc tcctgaccct gttatcatcg
    37561 aatcatgctg aaccgttcaa cataggtagt gattcgcctg aaatttcaat gcgtgagctt
    37621 ggacgtatgt tgctggaggt ggccggtagt aagcggaaag ttatccatac gccgagtgag
    37681 gaagtcgact atctgacgga taatcccaac cgccgctgcc cgaatcttgc gaagtctcga
    37741 tcgcttctcg gctatacccc gctggtcgat ctccggttcg gactgagcag aatgctgcag
    37801 tggtacaagc agttcgttgg cttggaagag ctggcgaaag atgaacgtgt gggctaacca
    37861 ttagggtgac catgaagata tcagtgatgg ggacaggata cgtggggctt gtatcaggga
    37921 cctgcttggc agagcgtggt caccaagtga cctgtatcga tatcaggtct gaggtggtac
    37981 aggaaattaa cgccggacgt ccaccgattc atgaaatagg attagataat ttacttcgct
    38041 cggcgcgcga caagggaatg ttgtctgcca ccacggatgc aaagactgcc attctcgatt
    38101 ccgatgtaac tctgatctgt gttggtacgc ccacggtgga tggtcggatg gacatgtcgc
    38161 aaattgtggc tgccgcaaag gaaatcgggt cagctctagc gagtaaacgt ggataccatg
    38221 ttgtggcagt gaagagtacg gtattgcctg gaaccacgga gggaccggtg aaggccgcca
    38281 ttgagtctca ctcgggcaag aaagtagggc acggctgggg cttgtgcatg aatccggagt
    38341 ttttgaggga aggtcgtgcc gtcgaggact ttcgtgttcc ggaccgcatt gtggtcggtg
    38401 ccacagactc catgaccgca gaggtgtttc taaacgtcta tgccgatttc acctgtccta
    38461 aattggtgac aaccccgcgc acggctgaga tgattaagta tgtatcaaat tccctgcttg
    38521 ccacgatgat ttcattctcg aatgaaattg gcaatatgtg ttccgcagta cccggtgtcg
    38581 atgcacgttt ggtgtggaag ggggttcatc ttgataggcg gctcacgcca atcaatggac
    38641 aggtaggtgg ggcggcagga gtaacggagt atctttggca tggtttgggg tttgggggca
    38701 gttgttttcc gaaagatgtg gcggcgcttc gaagctttgg gcgtaccgtg ggcgagcaga
    38761 ctcggatgtt ggacgccgtg ttggacatta ataccgacca gcctctccga atggttgccc
    38821 tgctggaaaa ggagttgagc ctcgcaggca agactgtggc cgtgcttggc ctcgcattta
    38881 aacctggaac ggatgatttg cgagaatcac cggctttgcc cttggtggct gagcttcgga
    38941 agaaaggcgc gaaggtgctc gtccatgacc ccattgccat gccccatgcg aagaagcgac
    39001 cggaattccg ggatgtcgtg tttgctgaag attgggctga ggcgttgagg aagagcgacg
    39061 cctgttgtct cgtgacggcg tggccagagt atcgaaatat tcagccgact aattttcaaa
    39121 aattgatgac ccgtgctctt gtgattgatg gtcgtggcgt attcgagcct gcagcaatgg
    39181 ccgcggcagg ggtcgtgtgg cgcggcgttg gatacacgcc agaatctacc tagagaaaga
    39241 ctcccttgag tgactgcacg cccgtatcgt tatcgttcta gccaaaggag aacaggtcat
    39301 gcttcgtgac ggatgtcgat tttgtgggac gcttctgcag cacgtctttc ttgatctggg
    39361 catgtctccg ttggccaatt catatttaaa ggcccatcaa ctaaaccaga tggagccgtt
    39421 ttatccgtta cgggcatttg tgtgtgagcg gtgtttgtta gtccaactag aagaatttga
    39481 gagccctgac cacatcttct ccgattacgc atatttctct tcgttctccg acgtgtttct
    39541 ccaacatgcc aaagattatg tcgatatggc cgtggaacga ttctcgctgg gtgctcatag
    39601 ttatgtcgtg gagatcgcga gcaatgacgg ttacctgctg caaaattttg tggcacgagg
    39661 cattcgcgtg ctggggatcg aaccggccaa gaatgtggct gaagtggccg tcaagaaggg
    39721 tgttccgtcc tgcgtgaagt ttttcgggac ccagacggca cgagagttag tagcggagga
    39781 aaggcgtcct gacttgatca tcggcaataa tgtcttggcg cacgtgccgg acctgaatga
    39841 tttcgtcgga gggctcaaaa tcctcttggc gcctgagggg attatcacga ttgagtttcc
    39901 tcatctgatg catctcatgg gcctgaatca gttcgatacg atttatcatg agcatttttc
    39961 ctacttttca ctgactaccg tgaaccaggt cttcgcgaaa catggcctga ccattttcga
    40021 tgtcgatgaa atatcgacgc atggtggttc gttgagaatc tacgctcgtc atgatgagca
    40081 tcattcaaag ccggtgaccg agcggttgat ggccctgaag aagaaggaag aaatacaggg
    40141 attcgcgaca ctcgctcact atctttcttt ttctgagaaa gcccgggaga cgaaacgagc
    40201 gcttttggag ttcctgattc gggcaaaacg tgaggggaaa tcagtagtcg catatggtgc
    40261 cgcggcgaag gccagtacgc tgctcaatta ttgcggggta cgccaggact ttgtcgacta
    40321 tctcgtggat agaagcacgt acaagcaagg acattggcta cccggggtgc atattcccat
    40381 tcatgacccc gaacgcatca aggaaacgaa gcccgactat gtgttgattc ttgcctggaa
    40441 tctcaaagaa gaaatcatga agcaaatgag ctacgtcaga aattggggtg gacagtttgt
    40501 tgtgccgatt ccggaagttc gaatatgccc ctgatcgatg tgtgagagaa gctcttttgc
    40561 cgagtgaatc ttggtgggtg aagatattga ggggcctggt gacgtgaaga gttttgatgc
    40621 aagcagagtt ttgccggacg gttgcggcga ggagatgtat cgatttatct ctgaactcta
    40681 tccgatctgt cgaagtatta caggacaggg tatccgggac acgttcgggc gcattcaaca
    40741 gcatatcccg ttgagcataa ctgccgtgcc aagcggaacg caggtgtttg attggatggt
    40801 cccgctcgag tggaatattc gggatgcata cgtggctgat cgtcaggggg tgcgagttat
    40861 tgattttaag caatccaact tgcatgtggt gagttatagc atcccgaccg cagcgcgtat
    40921 gccgctacgc gagctcaaag cgcacctctt cacgctgccg gataatccgg attggatccc
    40981 ctatagaacg tcctattaca aagagtcctg gggattttgt ctgagccatc gtaaatatct
    41041 ggaccttcgt gacgaggact atgacgtcgt gattgactcg tccttgaaac cgggacacct
    41101 cacctatggc gaatactatt tgcccgggga cactacagaa gaggtgctca tttcatgtca
    41161 tgcctgccat ccatcactgt gtaatgacaa cctatcagga attgcggtag cgacgttctt
    41221 ggctaaatcg ttacagactg gctcacgtcg atactcatat cgtttcctct tccttccggt
    41281 tactatcggg gcgatcacct ggctagccct caaccagcag aaggtgtcca acatcaagca
    41341 tggctttgtc ctgaccggcg tgggtgatga ggggggcttc acctacaaga aaagtcgaga
    41401 tggcaatgcc gacattgatg cggcatttgc ccatgtctta caacactcgg gagagtcgtt
    41461 tgcggtcact gaatttatcc catatggata cgatgagcgc cagtatggtt caccgggatt
    41521 taaccttccc gtcggatgtt tcatgcgtcg ggcgaatgga gggtatccgg agtatcacac
    41581 gtctgccgac aatcttgatt ttgtcaaacc gacatcactg tcgcgatccc tccaagtggt
    41641 ccagtcagtc gtagaggtgt tggagtcaaa tagggggtac gtcaacacgt ccccctattg
    41701 cgaaccgcag ctaggcaaac gtggtctcta tcgcacaatc ggaggaaacg tcacggggca
    41761 gagcgcggaa atggctcttc tctgggtctt gaacctgtct gacggggcgc acaccttatt
    41821 gcaaatagct gagcgagcgg ggttgccctt ttcaaccatc aagctagccg ctgatgcgtt
    41881 ggaaggtcag ggattgctac gtcctgctgg ttagggtgcc gagagcgtgc gcagatggga
    41941 atcagaactc tcagtgatgc tagccgcagt gcaatggttc ttggagggtc tgggtgtttg
    42001 tgagtgagcg ccgtgtcgcg gtccagttgc gaacgcgcat catcaagcgc gtaacgccaa
    42061 aggaggtacc gaatgaataa cacaatttac tatgatgccc aattgactga tgacgagaga
    42121 aggaaactga tttttgaagg gcagctggta gcgtattcac ctcgagagca ttccctagcg
    42181 ttagtggccc atgcacgcaa gttgatcgaa gaggcttttg ccccgctcga tcctgagacc
    42241 gcgcaatatc acttgccggt tgagcaatac gctgaagtgc taggtaaact gaagccacac
    42301 tttattcatc acccggattc caagaaacac ctgagagcgt tgttggcgga gatgggggct
    42361 gatcccgaga aaacttactt tgatgtcccc aaaatgcgaa gttcaaccag cgataactat
    42421 ctgacgaccg gaatagcgta tgcgtggcat ccgcatcggg atacctggta ttcagccccg
    42481 ccttgtcaaa tcaattggtg gattcctatt tacgacatcc gttcggataa tgcgatggcc
    42541 tttcatccca agtactggaa tcgggctgtc aagaatgatt cccgcagtca caattactac
    42601 gagtggaaca agcaacatcg gggtggacat gtctcccaat ttctgaaagc cgacccacga
    42661 cccctttcca agcctattga aacgattgag atcgatcccc aaattcgcat cattgttcct
    42721 gcgggcggaa ttattctgtt ttcggcggct cagctgcatt cgagtgtgcc gaatacctct
    42781 ggcaagaccc gattcagtat cgatttccgt gtggtgaatg tggatgatgc tgccgcccgc
    42841 cggggggcac ctcatgttga tgaagagtgc acaggaacga ccatgcgaga ctacctgcgc
    42901 ggaacagact tgtcccagat ccctgcggag atcgtcgcgc tctacgatga tggcgcgcag
    42961 gaagacggtg agttgcagta caagccgaag gatgtctcca ctccgtcttt gtaaacgtat
    43021 tgcttctatg ccaaatctta atcggtggcg tggcgaaaga atcctcgtaa caggaggcag
    43081 tgggtttctc ggatcacatc tatgtcaacg gttgctcgag ctgggggcgg aggtacacgc
    43141 gacttcacgc gttgagcgga cgtcggagcc gggtgggccg atttggtggc aggtcgatct
    43201 tacggccatc gatcgtgtgc ggaaactgct gacggacctg aagcctatgg tggtgtacca
    43261 tttggctggg tctgctggcg cgaagccgga ccttgatctc gtattaccta ccctcgaagg
    43321 gcttgtggtt agcgctgtta atatacttgt ggcgggaaca gaggctggct gtggccgtat
    43381 tgtgatgacc ggatcactta ccgaaccggc ttggggcgag cacgcaccga ttccaagttc
    43441 tccctatgcg gcggcaaaat ggacgtcgag cgcctatgcg cgaatgtttc acctgctcta
    43501 tcatgcgccg accgtggttc ttactccgtt catgacattc gggcccggcc aagaccgaag
    43561 caaaattgtt ccgtccgtca ttctttcact tctcaggcga gaatcccccc ggctttcttc
    43621 ctgtctctgg gaggtggatt ggatttttat tgatgatctc gtggaggcgt tcctggcagc
    43681 tgcggcagtt ccagacattg aggggggctg ttttgatgtg ggaacgggaa ctaaccggac
    43741 agtacggtcg gtggtcgagc ggattttctt actgatggga gagcagacgc aaccgctatt
    43801 cggtatgttg ccggatcgcc cgtcggaacc ggtgcgaagc gccgatacga tgcaaaccta
    43861 ccaactgttg aagtggcgtg cgcagatgtc cctggatgag ggcttgaggc ggacgattgc
    43921 ttggtacact actcatgctg ctatgggtgg tgcatgactg ccgcagcgat gcagcaggtc
    43981 ctgaagtggt atgcgaagcg aaaaattcgc gagctgtgtc ctttgagcat tgcggaccga
    44041 ttgttggggg aacagggggc cattcatcaa ctctggtcga tagggattgt caccggggcg
    44101 tcgcccttgc gtcttcgcca cgcgccaggg ggactcaatc cggtactgac tcgggaagat
    44161 atttcggacg tgagggcgat gttcgtcgcg gatccattcc tgcttcgagt aggggatctg
    44221 tggcacatgt ttttcgaggt atataatttt gatgctgatc gtggcgagat tgcctgggcc
    44281 accagcaagg atgggtttgc ctggacctat cagaaagttg ttttacaaga atcatttcac
    44341 ctgtcctatc cctacgtttt tgagtggcac ggccaatatt atatgattcc ggaaacccac
    44401 caggcggaga gtgtgcgtct ctacgaagct cggtcatttc cgacaaagtg ggagtgtacc
    44461 gacacgttgc tccggggcca tcgtttcaat gatagttcaa tcttttatca tcacggtcga
    44521 tggtggttat tcagtgaaac caaccctacc ttggctcacg atacgcttcg tctctatcat
    44581 gctcctgacc ttcgggggcc ctggcaggag catcctcgat ctccgatcat tcaagagaac
    44641 ccccatatcg caaggccggc gggacgagtc gtggttgtgg gcgatcgtat cctcaggttt
    44701 gcacaggact gttttccggt ctatgggaca agggtacagg cgttcgaaat tacggagctg
    44761 actccttcaa cctaccgtga gcagcccgct tcgcgtggtc cattattcgg tccgagttgg
    44821 aggctctgga cccaaggcgg gatgcatcat atcgattccc atcctctgga ttcccatcaa
    44881 tggattgccg cagtggatgg gtggcggcaa gtcgggtggc cgattcctga agagtggcag
    44941 agggcgacgt gctgaaacgg ttgtggcgat acttgcaacg gtaccaactg tatctatggg
    45001 gacgagaggt gattgctgcg gttcgccgtg tctggcctca gttccgtggg ggcgttgttt
    45061 ccttgcacgc cgcggggcgt tcacacggcc acgtgttgat ttcatacgac aatcatgggt
    45121 tgctctgtaa aatgcgaggg gaacccattc ccaccagcca ccctcagttt ctgaagacta
    45181 tcctcatggc tcagaccttt gtcgatttgg gctatcacgt agatgtgatt cactgtgaga
    45241 atcagagatt cattccctgg aagccgtacg atattgtggt tgatacacgt ctgaatttgc
    45301 agcgactaca accttatttg ccttctacct gcatcaagat tctgcactgt gacacggctc
    45361 agattgtcta ccaaaacgcg gctgaaatgg gaagaatgtt ggcgtttcaa cggcggaagg
    45421 ggctcacggt tccgccgaat cgattggaaa caccgcattt aggggttgaa cacgcggatt
    45481 atctcacgac ctgcggcaat gagtttacgg tgaacacata tacctatgcg gataagccga
    45541 tttttcggtt acccatggtt gtccagcaga tgtggccttg gcctgagacc aaagacttcg
    45601 atttgtcccg ccaccggttt ctttggtttg gaagtcgagg gatggttcac aagggactcg
    45661 acttggtgtt ggaggctttc gcgagaatgc cggaatgcca gcttaccatt gtggggccgg
    45721 tgcgcaatga gccagagttt gtgaatgttt atcggaaaga attattccac acgccgaata
    45781 tcacatatgt cggttggctg gataagtcca gtgaggagtt tcgaattgtc ttggagcaat
    45841 cccttgcaca tgtctttccc tcttgttctg aggctggggc ggcagctgtg gtagaaacca
    45901 tggctgcagg ggtaatccct gtggtgacct atgaagcttc aattgatgtg gagaattttg
    45961 gctttctttt ggaggacgct tcgatcgaaa ccatcatgcg tcaggtgcgc gagattgctg
    46021 cgatgtctgt ggatgaattg cgtcgcaggg ctaagaaagc gtgggaggcg gcccacaaca
    46081 ataacacgcc ggagaaattt gagcgttcct atcgtgcaac catagaaatg attttggcaa
    46141 agcatggaag acgatagcag catctgccga aagatgttcg cttcggggaa ggcggaggat
    46201 catgtcttcg ccgtcaagcc tctgtggggc gtagggagcg cattgacgaa tggctaagct
    46261 tgggcgccag ctgggaaagt tggcaaattt tgtgcttttg ttaggggctg cgctctgtgc
    46321 aggcgtgttc gctttcttgg tatggcgcca tggcatgtcg ctccgctatg tggtgttcgt
    46381 ggggcttgca gtgatactgg cgctcggcgt acgaatgccg gaatccctca ggatcaatag
    46441 tgtgcttgtc ggcgtatcca catgtgtcgc ggtatatctg gctgaattgc tcctcgccta
    46501 ttccggcaca gcaattacct ccttgggggc tcaagcgtgg ttaagcttcc ctcaggacgc
    46561 aaacgtacgg gttgcggctg aacgaatgaa gactgtgcag gagactcacc agaaattcga
    46621 tgctcgttcg cgcttggagg tgatcctgga tatgcgggca catggcgtca atgcgtatcc
    46681 ggatgtcttt ccaatggtcc tgtttgcacc gtcttccagt gacagtatcc aatccgtact
    46741 gactagccag catgaggaat tcttgcccgt ggctggaatg gccgctacga cgacggtatt
    46801 ttgcaacgag agcggggaat atgtcgtata cgaaagcgat gagcacggct ttcacaatcc
    46861 gcgtggcatg tgggagcata gaccggtcga acttcttgcg ctgggtgatt cctataccca
    46921 tggagtatgt gtcccgtcgg ataaaggatt tgtggccgtg gtccggggac atcatccgaa
    46981 tacgatgaat ttgggcgtaa atagccatgg tccgctcacg agcttagcga cgcttaaaga
    47041 gtatggtccg gttcttaagc cgaaactggt gctgtggttt tattatgaag gcaatgatct
    47101 cagagatctc gacgggtggg agaagaattc tccgctcttg atgaagtatt tgtcatcctc
    47161 attttctcaa cacttatttg agcggcaacc tgaaatcgat cgttcgttga gggactatct
    47221 tgatgccgca atggcaaagg cgaccgctcc gatctctctt gagaacatcc taaagcttca
    47281 ccatctccgt catgccgtgc agtcatttta tgagcgccgc ccagcagaac aggggtttcc
    47341 ggctgaattg ctggagttcc ttcgtcattc cggcgcaccc gctgctccgg aagaccttca
    47401 actgttcgag cgtattctgg ccgaggccca ggcgacggca aagacgtggg atgggcgtgt
    47461 cgtgttcgtc tatctgccca cgtgggaacg atatcgcctc cctgaattgg ccagcaagga
    47521 tcgcgacaac gtgctgggga tcgccaggcg gttgaagctg tatgtcatgg acatgcatga
    47581 ggtatttgtc acccatccgg atcccttatc gctttttccg tttcgacgat acgcccacta
    47641 caatgaagcc gggcataagc tcgtaggcga agaggtggtc aggcagttag ggaaactgtg
    47701 acgtctgccg atcgtgtgtg tcgaaatgtc gagccggtaa gtgtgttctg ctacatacct
    47761 gtttgtggcc tgcggtggct tcgatttttt gcgttcttga ggttgatcag agcagaatgc
    47821 tttgaaatgg acgggttgaa acggtgaata cacatcaaaa aaaagtagcc gtcgtagtgc
    47881 cgatgcataa tcggagtgag ctcactcctg atgagcagat ctcttttcag catctcacgc
    47941 attatctgca tgcgtatgac aagtatctgg tggtgccgaa ctcactcaac atcgacttgc
    48001 cgggctgttc gctcaagcgt tttggcgatg aatatttcgg gagtgttgcc gcgaatacac
    48061 gcctcttatt gtccgaaacc ttttaccgtt ccttcagtga gtaccaatac atactgatct
    48121 atcatcttga tgcgttggtg ttttcggatc agttagaagc ctggtgcgat acggggctgg
    48181 actacatcgg tcctccatgg attcactgtg cggacagccc ctgggtgaag gaagcacgtg
    48241 tgggaaatgg cgggttatca ctgcggaaga tcgagagttt cctcaaggta ttccaatcgg
    48301 acgtctattg gatggagccg gaagaatatt ggcgggaaag gtatgcgggg aagcctgcct
    48361 atgtccgaat gcttaatctg ccgaggcggt ttgcaaagcg gctatcctgg ctgaacaatg
    48421 cacgtcttga gatgtcacag tggcacctcc gtcctgacgg gaccaagaat gaagaccatt
    48481 tttggtctga tcgtgccaag cactatgtgc ctgacttcag agttgcctcg gtcgaactcg
    48541 gcctacgctt tgcgtttgag gtggcgcctc gcttgtgtta cgacatgaat caccaccagt
    48601 tgccatttgg ttgtcatgcc tggccacgct acgatcgctc tttttgggaa ccctatctga
    48661 ttacgccgca cgcggcttga tcgacagggc cagtgtagcc acagaatggg gtcggcgatg
    48721 gcaagacgtt ttgcgatggt cgtggcgctt ggtgtcgcca cggtggtttc tgctgcgatc
    48781 atgcttggtc ttggcgaact gacgattcgg tctatccatt tgctgagaga cggaattcca
    48841 tttttcgaaa gtgtcgacgg tggaaggatt ggacctatta gcctagatca ggaactcggc
    48901 tggaaagcga ccgaacatta ccaggagaca ttggtagaga aaaccaatgc aggtcggccc
    48961 tattcagttc ggcgctctca aaaacagtac ggatttcgcc agttcggcgc tctggactcc
    49021 aagaaaattc ggcttctggt gattggcgat tcgtttactc atgccacggc agtttcggat
    49081 gatcgtacgt accatgcttt attagctcag ctgttggacg ttgaagtatt tgcgtacggt
    49141 gctggtggtt acgggacact gcaggaactc atgatactcg atcggtatat cgataccatt
    49201 cgaccagacg tcattctctg gcagtattgc gcaaacgact ttatcaataa tgataatgag
    49261 cttgagcgat tgagccttgt gaataacaat gggtgggtca ggccttattt gcagaagggg
    49321 caggtgctgc tgttgtcacc gaaggagtcc tcacttcaag ttcgagagtg gatcaaccag
    49381 aggagtcggt tcctctattt tgttgtgagc cgtattgacc ggctgcgagc ggtgaggact
    49441 cgtgacacgg ttgaagtcgc tattgagtcg gaggggatgc ggcatcccgg atttcttcgt
    49501 gcggtagggg ttaccgatga gttgatgggg agggtccgca agaaagtcgg aaatcgaccg
    49561 attatggcat tcaattgcgt gcgagcggag ccctacgatc aagcatttcg tgatatctcg
    49621 gcccgtcatg gcattgccta ttgggacgat gtagcatcgg tagttcttga agcccaccat
    49681 cgtggagaag atgtctacgc tgcagatggt ggtcattgga atgagcgggg gcatcaattg
    49741 gcagcagagg cgctcgccag tcattttcgc gctgaaatga gaactggggc ccgcccttga
    49801 tgaatgtcga gtatagtgag tcgagaatag ggagggagta agcacatcat gaagcgaatg
    49861 cttgttaccg ggtcttctgg tctcatcggt tctgaagtgt gcgcatactt tcatagtcaa
    49921 ggatggtcga ttcatggagt cgacaataac actcgggcca cgttttttgg accacaaggc
    49981 gacacgcgtt ggaatcaacg caggctgcag gctgatctga aacagttccg gcaccatgaa
    50041 ctcgacatcc gagaccgcaa gggcgcattg gcgctcattc aagagctcaa acctgatgtg
    50101 attgtgcata cggcagcaca gccctcgcat gacctcgcgg ccaagattcc gttcgatgat
    50161 tttgatacga atgcggtggg tacgctgaac ctcctcgaag cgaccaggtt gcacacgcca
    50221 ggagcagtgt ttgtgcacat gtccacgaat aaagtgtacg gcgatgcccc gaatgagttg
    50281 cctctcgtgg agcaggataa acgttgggat tatgcgtctc ccaatgattt tgatggaatc
    50341 acggagcaca tgcgcatcga tcagtcgaaa cattcacttt ttggtgcctc gaaggtggcc
    50401 gcggatgtca tggtgcagga gtatgggcgt tatttcggca tgaagacctg ttgtttgcgg
    50461 ggagggtgcc tcaccggacc caatcattca ggcgtggagt tgcatggatt tttaagttat
    50521 ctcgtcaaat gcaatttaga aggtaagaag tacagcatct tcggctataa aggaaaacag
    50581 gttcgagaca atattcattc cctggatgtc gcacgattca ttcacgcgtt catcgagaac
    50641 ccacgaagcg gtgaggtgta caacctcgga ggcgggcgcg gaaacagttg ctccatttat
    50701 gaggcgttcg agatgatcga agctatttct ggcaagaaga tgctgtatga atatgtcgac
    50761 aagaatcgcg aaggtgatca tatctgctac atcagtaacc tgaagaaaat gaggacgcag
    50821 tatcccggct ggggcattac gaaaacgttg aagcacatct tcgaggaaat tcaccagtcc
    50881 tggctgaagc gggccgctgc ggtgaatgtg ccgatctgaa aaaactgtgc taacgtcagc
    50941 gttggaagaa atacatacgc catgaaggtc ctggtaattt cagcagccta tcctccgatg
    51001 catgcagggg aggctaccaa tacctaccac ttgtgccggc aattgactga gcggggcatt
    51061 gacatacatg tgctcacgtc ggttggcaat gttggtaccg atgatcctcg gattcgcgtg
    51121 tatcccatca tccagacatg gggctggctt gagttgcttc gggttcggtc ttttttaaaa
    51181 aactgctcgc cggatgctgt gtttctcatg tacatcggac tcatgtacaa atttcatccg
    51241 atggtgactt tcctgcccac gctttccaag gggttatttc caaacgttcc gtttgtaacc
    51301 cggtatgaga gtgcgtttgt gggggctgat ccgtcgaaga caggcattgt ttcgcgtttg
    51361 ttcaggaagc tgatggttca atgggctggt ggaaaggatg tggtgtatag ctcggggaca
    51421 ctgttgcggg atagtgatgc tgtcatcgca ttgtgcgaac gtcaccgtgc aatgttgctc
    51481 gaagaatggc ctcccgtcag ccagaaggtg gcgctaatcc ctccacctcc gaacttgttc
    51541 atcgcgtcga atgcaggggg catggcacgc gaacgaggac gaacacgatt gggagtgacg
    51601 gcaggtgaat tcgtcgtcac gtttttcggg tatctctacc cgatcaaggg aattgaaacc
    51661 ctgttgcggg ccttcgcgag cgtatccgcg caacgccctg aagcgcgact cttgttcatt
    51721 ggcggaaagg ttgggctgga cgttgaaggt ggagtgtcct atttcgatga gatgcaggcc
    51781 cttgccaagg agttgcacat agacgggttg acaacatgga caggtgcgtt caagtcagaa
    51841 gaggaggacg cctcgctatt gcttcacgcg tcagatgtgt gtgtgttgcc ttttctggag
    51901 ggggtgcaac taaacaatag ttccttcgct tctatggttg cgcacgggct tcccatcatc
    51961 gtcacacggg gtcctctgat ggacaaggca tttgtccatg ggaaaaatgt tcttacctgc
    52021 gaaccaaaag atcatccggc cgtgacgaaa ctcctgcttg aagtgatgga ccgtgcggat
    52081 cttcgtgagc gtcttcgatc tggtgctgtc aagctcgccg ccgagtggtt ttcctgggag
    52141 acggcgacgg agaaaacact gaccgctttg cgaccgcggc tgtcggacaa ggttgtatag
    52201 gtcgcttttc ctgggtacat tcccacatgc ctcgtgtatc cgtcgtaatt ccgacttata
    52261 actgtgccaa atttcttggg cggactatag actcggcgtt gagacagacc tatcgagatt
    52321 tcgaaatcat tgtcgtcgac gatggatcga cggatggcac ccaagctttg gtagcggcct
    52381 acgaagagtc ggtacggtac gtctatcaac ccaatcaggg tgcttcggcg gcacggaatg
    52441 cagccctgtc gagggcgagc ggagaattta tcgcttacct ggatgccgat gatctttggc
    52501 gccccgacaa gttgtctcgg caggtcgaat ttcttgatgc gcattccacc tgcggatttg
    52561 tgcatacaga ggtgtcggta atcgatgaac aggacacagt cctgcacacc tgctttaatc
    52621 aggacaccaa aagacctatt cctcaggggc aatgtgtcag ggatctgttg ctgcggtctc
    52681 atatccagac gttgacggtg gtagagcgtc gcaccgcatt tgacgatgcg gggaaatttg
    52741 atccgcgcct gccgattgct caggattatt tgcattggat tggtgtgggc ctgcaggggt
    52801 atggagttgg atatctgcct gaaccgctgg gccaatatcg ctggagggcg gggagtctca
    52861 tgtccagcca gcgtcggctt ctgggagatt ttgtgaagat atacgaaatc ctctcgacgg
    52921 agtatggtct tgaacggtcg caggggcctg agatcatgca actggtgcaa gcgcagctgt
    52981 acgcaactca gcggcaactg gcgtatctcg agcgacggga atgttcaggt gcagcagcgc
    53041 ggcaacggct tggtgcccta atccgagagt ggcccctaaa tcttgagctg tatgtggact
    53101 tcgccaagac ctatgtcagt cggcaagtgt cctgacggca gcgaatcctc tggttcaaca
    53161 gcgaacgcag ataggcggtt tgtaggagta ggtgcaattc aactatgaac attatgctgg
    53221 tcactccttg gcgaccttcc ctcaccgggg ggatcagtac cgttgtcgca cggttgacgg
    53281 gggaatttca gaagaaaggt cacaatatca ccatttttgt cgccgatcgc gagaatcggc
    53341 ttcgtcaaat tgaagcactg gatgaaatcc cagtctatgg catgtatctc cggtcacctg
    53401 tctcgtcgca gtatcctctt cgtgccatgg tcatgtgttg tatttggttt ccgattacga
    53461 tggtgcaact catctggctg gctaggaaga aacggctgga tgcagtttta attcagtatc
    53521 cgcttcctgc gatgttctat tttgggatat tgaaacgtgt attcggagga actcttttca
    53581 tcacctatca aggaaacgat gcgcatgatt tatgtctatg ggatcggcga gaacaacgat
    53641 tggtccggtt cttattggag aaggctgacc tggtgttggg tgtctcacga acgctcttgg
    53701 ccaaagttga atcagttttg cagggcgtcc atcttaggcg cagtcggcag ctgcccaatg
    53761 gcgcaccgct tgacgttatc cgggcggtta aagcaggtga ggcgggttct gatctgcctc
    53821 caagctatct attgaccgca ggccacctga ttcagcggaa ggggatagac ctcgtgatcg
    53881 ccgcattgcg ggaagcgaaa gagcgcggtg tggtgttgca gctcgttgtt gcaggtgatg
    53941 gaccagaacg ggaaaatttg atacgccagt cacgcgagca gggtgtgtca gatcaggttc
    54001 ggtttttggg caatcaatcc cataggcagg ttctcagtct gatgaagacg tgccttgctt
    54061 tcgtgctggc ctcgcgagca gaggggatgc ccttggtcat cgcagaggcg atggcatgtg
    54121 ggaaagctgt cattgcgagc aatattgacg gtgtgccgga aattgttcat gacggcacaa
    54181 ccggaattct ggtgccagca gaggattctg gagctctcgc aacaggtctg atcaggttgt
    54241 gctctgacgt agcttttcga gagactctag ctaagcaggg aaaagagtgg gcctgtcgag
    54301 aatacaactg ggaagcgatt gcgcatcgat accttggctt catcgaggag tgccaggccc
    54361 accggtaaat gtcatgatta taaccatctc agtaaaagca ggggctaccc cctctgacag
    54421 aggactgtgg gattgcttaa acggtgctag attagtgcag agacacattt cttttacagg
    54481 cggggaacag cgatgaatat tcttggtatt tctgcgttct accacgacag tgccgcatgt
    54541 ttggtgcgtg atggggaaat cattgctgcg gctcaggaag agcgcttcac gaggaagaaa
    54601 catgatcccg ggtttcctca tagagccatc gactactgtc ttcgtgaagg acagatagag
    54661 ctcaaagatg tgcggcacct cgtcttctat gacaagccgc tggtgaagtt tgagcgattg
    54721 ctagaaacct atcttgcttt tgcgcctagg ggtgttcaat ccttcgtggc cgccatgccg
    54781 gtgtggctga aagagaagct tctgttgaag agtttgctgc agaaggaatt tctccagcat
    54841 gccccggacc tgacgacatc ttccctgcca cagattttgt tttcagagca ccatgaatcg
    54901 catgcggcat ctgcattttt cccctcacct tatgagaagg cagtggtttt gtgcatggac
    54961 ggggtcgggg agtgggccac tacgtcggcg tggcttgggc aagggaacac gttgactccc
    55021 ctctgggaaa ttcctttccc tcattccatc ggactgctgt actccgcctt cacatactat
    55081 acgggtttca aggtgaattc cggcgagtac aaggtgatgg gtttggcgcc ctatggcgag
    55141 cctaagtatg tgaaagcgat ttacgaacat ttgctggacc tcaagccgga tggaacgttc
    55201 cggctcaata tggattattt caattattgc accggactga caatgaccgg cggcaagttt
    55261 gatgatgttt ttgggggacc acctcgcaag cctgaatcca agctgactca gcgggagatg
    55321 gatttagctc gttcagttca agaagtgaca gaagaggtca tgctacgtct gtcgcggacg
    55381 ctccatcggg agaccggagt agactatatg tgtatggccg gtggtgtggc gctgaattgt
    55441 gtcgggaacg ggcgtattct tcgggaggga cccttcaagg gtctctggat acagcctgct
    55501 gcaggcgatg ccggaggtgc gttaggtgct gcattaactg cctggcatca gtatgaacaa
    55561 caaccgcgca aggtgacttc cggaaaggat ggtattaagg gatcatattt aggtccctgt
    55621 catagcaacg aggaaattga agcatatctg aaaaaggttg atgcgccgta tgtcaggctc
    55681 gatgagaatc aactctatgc ccgtgtagcc gaagaacttg ctgcagggaa ggtcgtgggt
    55741 tggctacagg gacgtatgga atttggacct cgggcattag gaggccgtag catcctaggc
    55801 gatgcgcgca atcgggacat gcagtcggtg atgaacctta agattaagta tcgggaatcg
    55861 tttcggcctt tcgcaccgtc ggtcttgcgc gaacgagtct ctgactattt cgtcctcgat
    55921 gccgacagcc cctatatgct actcgtagct cctgtcgtag aaaaacgacg tcttcctgtg
    55981 agttccgcac aagatggatt gtgggggatc gaactgttga atattccgcg gtctgatatt
    56041 cccgctgtga ctcaccttga ctactcagcg cggattcaaa ctgtccatca ggacacaaat
    56101 ccacggtact atgcgttgct gaaagctttt gaagcaaaga cggggcatgc cgtgttggta
    56161 aatacttcct tcaatgtgag gggggaacca attgtcagca cgccggaaga tgcctatcga
    56221 tgttttatgc gaacggaaat ggacgtgctc gtgctggaga attgcgtgtt gcttaaggcc
    56281 gatcagaagc cgcttgaagg cgattcggat tggaagaaag agtttgagct cgactaacca
    56341 gtaagatagt gaaggcgaag gaggaattga ttcatgcgaa ttctgattac gggtggagca
    56401 gggtttctcg gtagtcactt atccgatctg ctgattgggc agggacatga tgtgattgcg
    56461 ttggataatt tgatcaccgg acgagcggag aatatttctc acctgatcgg aaatccgaaa
    56521 ttcagctttg tgaaatacaa cgtgtgcgac tatttgcatg tcgatgggca attggacgct
    56581 gtaatgcatt ttgcgtctcc ggcgagccct caggattatc ttgagatgcc cattgcgact
    56641 ttgaaggtgg gggccttggg aacacacaag gcgttgggat tggcgaaagc caaaggggcg
    56701 cggtttctat tagcgagcac ctctgaagtc tatggcgacc cattgctcaa tcctcaaccg
    56761 gaaacctact ggggtaatgt gaatcccatc ggcgcgcgtg gggtctatga tgaggcgaag
    56821 agatttgctg aggctatgac gatggcctat caccgatatc atggagtcga cacaagaatc
    56881 gttcggatat tcaataccta cggcccaagg atgaggccga aggatggtcg ggtcgtctcg
    56941 aatttcattg tacaggccct tcagggaaaa ccgctgacgg tctttggaga cggctcccag
    57001 acacgcagct tttgctatgt cgatgatttg gttcgtggta tcgtggcgtt attgatggtg
    57061 aaatcagaca agtctgtggc ggaacgcact gatcgtacga agttcctcac tcagaagccc
    57121 gacgctatcc tggacagcat tcatgatccg gttaatatcg ggaatccacg agaacttacg
    57181 gttcgtggta ttgctgagat cattctgaaa attaccggtt ccaagagtgt aatcgaagag
    57241 cgtccgctcc cagcagatga tccgaaggtt cgtcggccgg atattacgag agctaagagt
    57301 ctcttagact gggaaccgaa agtagaactc gaggatggta ttcggaaggc cacggagtac
    57361 tttcgccagg ttattgcgaa gtaatgggtg ttcggtgacg ctaggctaga aagggcaggg
    57421 ggagcagata gttattatgt gtgggattgc gattgctata gggctgaatg gacggcccat
    57481 cgagcgagtg gccgttgagc ggatggccaa aagtttgcgt catcggggcc ccgatgacgg
    57541 tggcatctat ctggatggtg ctgtgggaat gggatttcgt cgtctttcga ttcttgattt
    57601 gtccgaagcg ggccatcagc ccatggtcag tgaggatggg cagtatgtgc tggtcttcaa
    57661 cggcgaaatc ttcaattatg tcgaattgcg atcggaactt cgtcaattgg ggtaccagtt
    57721 tcggtccagc ggggacagcg aggtcctgct tgcggcatat cgtgaatggg gtcgtgagtg
    57781 cttgtccaaa ctcaatggca tgtgggcgtt tgtgatctat gatcgtcgac atcggctgat
    57841 ttttggctcg cgcgatcgat ttggtgtcaa gccgctgtac tacagtcgta gctccgatgt
    57901 gatgcaattc gcttcggaga ttaaggcgtt gcgagcatct ggctatcagc cgggtgagat
    57961 caattggcgg actgcggcca ggttcctgtt cgagggacgc ctggacagcc aagttgagac
    58021 gttctaccaa ggtatcgagc agattcctcc ggggagcggg tttgaactga cgctggatgg
    58081 cacctggcat caatggtctt tttggtcgct tgatgagtta cctctgagtg catccaagaa
    58141 ccctgcagcg acgtttgcag agctttttga agatgcggtt cgaattcgaa tgcgaagcga
    58201 tgtcccagtg ggagtatgtt tgtccggggg gcttgattcg actgcgatta tttgtgctgc
    58261 ggctcgtcag cgaggtgaga cggctgttgg gcagtccgaa tctctccagg cgttctgtta
    58321 tatggcaaag gagttcgacg agtcgaaata cattaccgat acgttgactc aaacacgcgc
    58381 acaactcagg cagctcgaaa ctagtcccgc agagctatgg agtgacttgc gacaactcct
    58441 ctggtttcaa gacgagccgg tgcacacgat gactgccgta gtgggctacc agctcatgcg
    58501 tctagctgcg tcccatggaa tccgtgttgt attaaacgga cagggagctg atgagaccat
    58561 tgggggctat tccagttatt tccaggacta ttgggtatca cttcttcgac aggggcgagt
    58621 gcgagaagcg tggcaggctg tgacgggcta tacagagggt catggcggca attcacgtca
    58681 gcgtctagta gcggctgctg cccgatgtct ctcatgggaa atgtataaat tccatgcgta
    58741 tcgcagttgg gcacaggctc ggcgccaggc acgtcttcgt caaaatccgt ggtttagtga
    58801 ggatctcacc aagcatttca tcaatgaaga tgtcccgccg gcctctacga cattgtcgaa
    58861 ctcattgaag cagtcagttg tttccgcgcc gttgcctctg tacttacgga ttgaggatcg
    58921 caactccatg gcccactcgg tggaagcacg gcttccgttt ttggactatc gcctggtctc
    58981 attcgtgtca ggcctttcag atgactggaa gatacgtggg ccctggaata aatatgtgct
    59041 ccgagaaggc atgagggggc ggatcccgga gtcggtgcga tcacgtgtgg acaagatggg
    59101 ttttcccact gccagtaaga agtggtttgc gcatgatctc tatgagccgc ttcgcgatat
    59161 cctggggagc caggccgttc gggagcgagg tatctacaac attggtgcat tgacggcgga
    59221 tttggagcgt caccaccgag gagaatcaga cttcgccaat agactattcc atgttgcgga
    59281 attcgagatt ttttcagatc tcctacgtca cgattcaggt ctcgctgcct aacgatttat
    59341 tcttggtgag tgccgttagg ggtcggtctc tttcctattg tgggatggcc atgtttttga
    59401 acgtatcgtt catctccact gtgatcaaac tcgtgttcgc caggttgccg aaggtctatc
    59461 cagcaaacga ggcctcgatg ggggaggggt ggtgacgctg tgaagcgagt cctcatcatc
    59521 gcctactatt tcccgccggt tgcggcgagt ggcgcaatgc gcccgcttgg tttctgccgg
    59581 aatcttccaa catttgggtg gcagccttat gtgctcacaa cctctccgga gtgtgtatat
    59641 ccggcacacc aagttgatga gaagcttggt gaacgtgtgc cggcgacggt aggagtgact
    59701 cgtgtgccct ataccgatcg ccttcagcag atattgtact atcgtgacgt actccggaga
    59761 atggtgaaaa gacaggtggg gcaggatagg acagggaagc tgcaaacaac ccaggctcct
    59821 ccctcgccgc ctcaatcgga agcaggccgt tcaacggtga aagatttttt gctggattgg
    59881 gcatttgcat ttccggatcg acaatgtggc tggttgaggc aggttgtgca acattttgag
    59941 caattcgacg aaaagcagat tccggacgtg gttttcgcga caggcgggcc gtggacaagc
    60001 tttttggtcg gctcaacctt ggccaagcag ttcaatcggc cactggtact ggactatcgc
    60061 gatccatgga attgtaaccc gtattattcc ttcagttctc ggttcttgac caggaaatcc
    60121 caagcggttg aggcgagagt ttgcaaggct gctagtcatg tcatagctaa tacagaggaa
    60181 cttcgccaac gactcattga ggaatatggc gaactccgag gtcgctgtac ctggattccc
    60241 aacgggttcg atcgggacgt gctgcctctt gggcagatcc ctgctgagca attggatggc
    60301 ctctctccct cgacagggta cgaactgtgt catttcggga cggtctacgg gaagaggact
    60361 ccgcgtgtct tactgcaggc aatgtgggag ttatttcaag agggttggtt gaagcctgaa
    60421 gcgatccgat tgcgatttgt gggcggatgg gactcaaccg atcaggagtg tgagcgatat
    60481 gcagcagagc tagagaaacg ctcttttttg cgacgcgagc cgtcgatctc gcacagcttg
    60541 tgcctacaag agatgcaacg atccagcgtg ctgttggtcc ttcagcctga ctcccctttg
    60601 caggtaccgg ccaaaattta cgaatatgtt gcgactggtc gaccgctgct attgatcggt
    60661 ggggagggtg caaccgccaa tctagtcact cggcacgccc tgggtgtaag tagcccgaat
    60721 cagctggagg cgattaaagt gcttctcaac gagctggcaa caggagcgcg aaaactggct
    60781 cagcctgacg tgacgcgtgt gaaccgtttc gattatcgct cgttgaccgg ggacttggca
    60841 gctgtactcg atgtggtcat tggggcaaaa gaatgttcgt agtgttgtcg ttatgagtac
    60901 gatggtctct tcaatacttg gccgaaccaa tgccgaaatc ctcgcatggg aaagttatgt
    60961 gctaagttct cccctcgcga cggggtatca tcagtccggt tggcgccgcg tgattgagga
    61021 ggcttttggg catccaaccc actatctggt tgtgcacggg caagacggtg tactacaggg
    61081 cgttctgccg ctggtttttt tggcaagccg agggtttggc cgtttcctcg tatccctgcc
    61141 gttcgtaaac tacggcgggg tggttgcaag ttcagcggaa gcacatcgct ttttggaagc
    61201 gcgtgctatt gagcaagctc gcgaactaaa cgcggaccat atcgagcttc gacaccaggc
    61261 gctgatggac acggcttggg tggccaccga acgaaaagtg tctatgcggc ttccgttgcc
    61321 gagctcgtat gagcagctgg taaagggatt tccctccaaa ttacgaagcc aggtccgtcg
    61381 ggctcaaaaa gaaggcatga cggcacgggt aggggggagt gagtgtctcg atgagtttta
    61441 ctcagtcttc actcgatgca tgcgtgattt agggacaccg gtttatgcga agggattctt
    61501 tgcaaagatc cttgaggtgt ttccaaagga atcgaacatc tgcattgttt cccacggtca
    61561 gacgcccgtt gctgcaggtt tcctgtacgg attccggtct tctttagaaa ttccttgggc
    61621 cgcgtcggat aaacgcttta acaagctggc tccaaatatg ttgctctacg gcacggtgtt
    61681 ggaatatgcc tgccaacggg gctttcaggt ctttgacttt ggccgttcaa gtcccgacag
    61741 cggaacctat cgattcaagg agcaatgggg agctcagcca cacaagctgt attggtacta
    61801 ttggatgaaa gatgggcgtc cggttcctca gctcaatccg caaaatccga aatttgctct
    61861 cgcaattcgt ctgtggcaga aattgcctgt ggtagtcgcg aatctgatag gcccttacat
    61921 tgtgaagcat cttccatgaa agctcagcga acgcttcctc catcggccgc accgatcgaa
    61981 tggcgagatc tcattcaggg gttgatgggg ctcgtgcacc cgcacgaaac cgttgggcag
    62041 ctacaggctg aatttcgcca atacttcggt gtgaagcatg tctggtttgt gtcatcgggc
    62101 aaagctgctc tcagcctcat cttccgagca ctgcatagtc tatctgggcg cagtaaggta
    62161 gttcttccag gatacacttg tttttctgtg ccgtcggccg ttgttcgtgc tcgattgtcg
    62221 gtggcgctgt gtgacgtgga tccgctgtcg ttggatttcg actttgcgca actttcccaa
    62281 gtggctgatt ccaacgtctt gtgcgtgttg gccactcatc tgctgggaat tggcgttgat
    62341 gtccctagag tcgtcgagct gtgtcgtcaa cggggcattt ttgtcgtgga ggatgtggcg
    62401 caggcgtttg gtggtgaacg cgatggagtt ccgttcgggg cgatgggtga tgtttcattt
    62461 ctgagttttg ggagagggaa gaatattacg tgcggttcgg ggggtgccat tcttacgaat
    62521 gatgatcgga taggtgaagt cttggctcgt gaatatgcac tgcttcccga gatgtcgctg
    62581 gctgacatgt tgaagaattg gcttgaggtg gcagttacga aggtgttgct taacccttcc
    62641 ctctattggc taccggctgg acttccgttt ctaaagctgg gagagacaaa gttttacact
    62701 gatttcccaa ttactcgtct ggacccgata cgcgccggat tgctgcgtcg ctggaaacgg
    62761 cggctagcca attcgaccac gagtcgagta gctcattccg agcagatcct ccgctcgctg
    62821 accctgtcga atgtgcagac tattaagccg tctggacgag ggcagtcagt gtatctgcga
    62881 ttgcctgtgc tgatgcgttc gaggcaggaa aaagatgcgg tgtgtcggac ctcagccgaa
    62941 caggggcttg gaatcagtcc cctgtatccc tcatctcttc agtacattac caaactccgt
    63001 gacacacttt catctcagga tgtgccccag tcgactatga tcgctgaacg gcttgttacg
    63061 ctcccaaccc acgaattggt gtctgactcc gacgtcgttc gaatttccac tgttattcaa
    63121 gcggctcagc ggggtgatgg cgcagcaacg attcgtcgtc cggatattac gaacgggcag
    63181 cgtcatgccc ctgaactgcc tcgaaatatt taggtgatcg ggcgattttg gcttgagcaa
    63241 cggcagactg ttgaaacggt aggaacctga atcatgtggt ttgtggaatg gattttctgg
    63301 ggttcattgg tattcatagt ttatgcctat gcaggctact tgcttgcttt gttggtcctt
    63361 tcctgtttca gaaatagacc cgtgctggtc ggcgatattc agccgagcgt ttcgtttgta
    63421 ataacggcct ataatgaaga ggcgcgcatc aaagagaaga ttgagaatag tcttcaccag
    63481 cagtatcccc ctgagcgcct tgaaattgtt gtggcatcag attgctcgtc agaccggacc
    63541 gacgacatcg tacgatcgta tgcggctttg ggtgtgcggc ttgtgcgtgc ccaggaacga
    63601 agaggcaaag aagcggctca gaaactggcg gtcagtcaga cgaacggaga aatactggtt
    63661 ttctcggacg tcgctacgac tttgcctcct cagggcattg ccaatattgt gaagtcgttc
    63721 agtgatccca cggtcggctg tgttagtagt gtggaccagt ttgtcgatgc tcagggcaat
    63781 ctcagtggag agggcgcgta tgtcaagtac gagatggtgc tgcgtcattt ggaaacaaag
    63841 gtaaatacgc tggtcggtct cagcggatca ttctttgccg ccaggaggac ggtctgtagt
    63901 ccttgggcag acgatctgca aagcgatttt aatacgttgt tgaattcgat gaaggccggg
    63961 ctacgtggtg tgtcggatcc agcgagtgta ggctactata aaaacttaac agatgagaaa
    64021 aaggagtacc agcgcaaagt ccggaccgtc ttaaggggga ttgctgtgtt gatgcgcagc
    64081 ctgccaatgt taaacccgct tcgatatggc atctttgcct ggcaactgtt cagccacaag
    64141 ctatgtcgat ggttagtgcc atttgcgatg atcggcacgc tgggctcaaa tattgtcttg
    64201 gcaataagtt ccagtccgta ccgggttctt ttgctgggac agattttatt ttatgccatt
    64261 gcaattgcct atgctggctg cagctggatg ccaaagagca atgtgtttcg attgccctcg
    64321 ttttttgttc ttgtcaacct ttccatcatt gatgcctgga tgcggtattt tcggggggac
    64381 cgggtgtttc gttgggagcc gtccaagcgc taagcgcgga tgggcagatt gatcagcgca
    64441 gtcgcgtagg aaaacatagt tatagcgggg tactgaatgg ataagatggt tgaaaagaaa
    64501 gagttgcgaa actttgggtt gattgtcggt ggtgttttta ttctgatcgg tgtctggcct
    64561 atgatccgct atggtgaagg gattcgtcta tgggccatcg tgccgggatc actgttagtt
    64621 ccattgggat tagccgcgcc gaccattctt gcgcctcttt ttaaggtttg gatgaaagtc
    64681 ggccacgtga tgggatggat caataccagg atcattttag ggattctgta cttcggactc
    64741 atcaccccga tgggggtgat catgcggatg tttggttggg actcgatgcg aagagcgctg
    64801 agccgagatg ccgaaagtta tcgagttgtg cggcaggcga ggccgcgcaa ccacatgacc
    64861 aggcaatttt aagcaaacgg agggcactat gggcgagttc ctaacagagc tgtgggcctt
    64921 tatgaaagaa cggaaaaaat tctggctgtt gccgattatt gtgatgctcg tgctattggg
    64981 cagcttgatc gtgctgacgc aaggatctgc ggtggctccg tttatctaca cgttgttcta
    65041 atttcgagga ctcagctgtt gagatgaact tctcggtggg acggttaaaa aaaacaattc
    65101 ggtctgcggt agcagggaca ttccacgctt catctatcta tcgatggcgt catcgaggca
    65161 aggtgtcagt gctaatgtat caccgtgtgc tgacgaaaga cgaagtcgcg cgttatccgg
    65221 ttcagccagg aatgtatgtg cttgacagcg tatttgctca acagatgtct tttgtgagga
    65281 gtaattttac ggtgctgtcg ctccaggaac tcctggatct gtggcaacgg ggcgagtgga
    65341 gtgcccatgc gcggtattgc gtgattacct tcgatgatgg ctggttggac aactatcgcc
    65401 atgcgtatcc agttttgaag cagctgcgta tacctgcgac gattttcctt cccactgact
    65461 acgtaggaag gaacgagtgg ttttggcctg atcagctggc ggttttgtgt aaagttattg
    65521 cggatcggaa acagcgtcag gaacctctgg cagcggttga gggtgtgttg tccggtttcc
    65581 tgagtgaaga tgcttctcgg ttggttgaag cgtcggttcg ccaagaacaa ctgggggacg
    65641 aaattatcga gcgttgtaag catttgccga tagagcgcat ccgagaattg gtgcaggcgt
    65701 tggttgcaga actacgagtg acattaccgc tggatcgggt gattgtaaat tgggacgagg
    65761 tgcgtgaaat gtctcgggga ggggtgtcat tcggttccca ctcctgcaca catcgtatta
    65821 tgacgaccat cacattggac gaagtttccg aagagctggt gaggtcaagg caggttcttt
    65881 ttgatcaggg tgtcaacttt gtgccggtct attgttaccc gaatgggaac agcgacgatt
    65941 caattgaaga tctagtgaaa gctcatggat atgaggctgc agtgtcggtg cgaatgggct
    66001 tcgaagggag ctgcccagaa aataggtatg ccattcgcag ggtaggaatc cacaacgaca
    66061 tatcggacac catcccgctt ttttctttcc gattgttcgg tccccaaccg agttcagtgt
    66121 aacgcaacaa gggcaatgga tataaggggg aatcgctttg gggcggggat acttgtgaaa
    66181 gtattgatta ccgacggaaa tgagcgggct gcgcttgcgg tcacgagggc acttggaggt
    66241 gaacaagtcg aggtgattgt cggggcagag tcccaacgat cccttgcagg ttcctcgcgt
    66301 tactgccgac agagcattgc ctatccgtct ccttatcaag aacctgaacg tttcatcgcc
    66361 acattgatgg aggcagtgca aacccatcga gtcgatgcct taattccgtt gtccgatatt
    66421 gccatgcatg tcctcggacc agagaaggct caattcgaaa aatatacgca tttcccggct
    66481 cccagtcccc aggcatttca agaaatctcc gataagtatc gattgatgca acaggccaag
    66541 gcggcaggtg tggccattcc cgataccctg tttgttcctg atggccgggt tgaagaggtc
    66601 ctgcgggaaa ttgcggattt tcccgtggtg gtgaagcccg gctgttcatt ggtaaaagac
    66661 ggcaggcgat ggacgaaaac gagtgtgtgt tatgccgagt ctcccgagga actcctacga
    66721 ctctaccgcg agagggccta cttacggcaa ccgtcattga ttcaacggcg tgtgatcggt
    66781 gaaggccagg gattgtttgt gttgatgcag gaggggatgc cgctgggtat gtttgctcac
    66841 cggaggcttc gtgaacgacc tccttctggc ggagtcagcg tgctgcgcga aagtattgcc
    66901 ttgccgaaag ccatggttga ggctaccctc aagctattgc agcctgtgaa gtggcatggt
    66961 gtggccatgg tggaattcaa agtagatacc gccactcagc gccccgtgct tatggaaatt
    67021 aacgggcgat tttggggctc tttacagctg gctgtggatg cgggcgtcaa ttttcccctt
    67081 cacttactca atatggctat gggagtgtgc gagacgatac ctgaaaatgg atatcgcgtc
    67141 ggagttaagt cccgatggct gcttggtgat ttggatcaat tggtcatgcg catcagaaag
    67201 agtgatcgtg cattgaatct tccgcctggc gccccatcca ggcttcaagc agtcctgtcc
    67261 ttctgccggt tttttgaccg aaatacattt tatgaaattg agcgtattga tgatctcggt
    67321 cctagccggt ttgaggtcat gcgatatgtt aagttggcat agggagtagt gatgtcccaa
    67381 gttgattctg cctccctgcg aggagtagct cgttcgatga agagggctgt gaacgaggcg
    67441 ctagatcact ggcgctatcg gagcctggtt aatagccaag cttttccgag ttccgtgcgt
    67501 gccattttgg tcgtctgtaa gggcaatatc tgtcgcagtc cgttagcaga ggcctatctc
    67561 aagcatcggg ttgaaaagca tgggttgccg attgcgattg agtcggccgg ccttgaaaca
    67621 tcgtttggaa aggcggcgca tccgctggcg caagtcgtgg gtacgcaggg gggactttta
    67681 ctcgccaaac atgccacgca acagctgcat aaggaacagg ttgagcgggc ggacatgatt
    67741 gtggtgatgg agtggcggca gcgacgccgt atgctcaagc tctatcccca ggcaaggcag
    67801 aaggtgtttt tgcttcggca gttttacgat cagtcggtgc gagaagtcgc cgatccttac
    67861 agtggaacgc tggaagattt tcagacttgc ttctcgatga tcaagcaggc ttgtgatgta
    67921 ttggtgatgc agatgttggc ctcgggcaag caaagatagg atcgagttga tttgatcgaa
    67981 atgtgagcgc agatggcaag gatcaaagga ggcccggcat cgacatgagt ttgcgaacta
    68041 gactgttcaa catctattgg acgctgcggg gcgccattgt gccgacgctg agatattccc
    68101 agtactccta tgaggaatcg ctgaaacggt atgtgacgcc atcagttgaa tgggtggaaa
    68161 taggctgcgg acactccatc cttcccagtt ggcgagcgaa tgaagaacgt cagttggcga
    68221 aaacgtgcaa ggcgattttc ggcatcgatt acgatcttcc ctctctcaaa gcacatccca
    68281 acatcaccaa gaaactgcgt ggcgatgtga ccaagttgcc gttcaagagt gaggcgttcg
    68341 acctggtgac cgccaatatg gtggtcgagc atctcgacaa tccggatgaa cagtttcggg
    68401 agatcagccg tatcctcaag tcgaaggggg tgttcctatt tcacactccg aacgcatacg
    68461 gttatggagt gattttatcc aaggtcgtgc ctgagtggct caagggaaag ctgatttacc
    68521 tgctggaggg tcgtgaggag catgatgttt tccctacgca ttacaaggcc aatacggagg
    68581 ctcaggtccg agcacttgct caagctaatg gcttcgaggt tctgcagctt gatttgcttg
    68641 ccacggatgc catcttcgcc atgttgccac cgctggccgc tttagagttg atatggattc
    68701 ggctgcttat gactcagcca ttccgtaacc tcagaacgaa tatgattgtg gcgttgagaa
    68761 aggtcgtcta atgctgagca gcgagcctcg tatgagaaat tgttcggtct aggaatgtga
    68821 atgatggtag tcgcgaagcg acagacgttg atgccggcag agaaagctct tcacctggta
    68881 ggcaatcgac cattgcctac cgagtcgaat gttggatttt atcttgtcat gttggccctg
    68941 ttgtttgaat ttggtaggcc gcaggatatt tttccaccgc ttaaagttgt tcccatccca
    69001 acgctccttg atgtgtcaat ctttttggca gtcttagttt ctggaaaagc taccattgca
    69061 aatgagcaaa ccaagctttg gttgatactt cttgcattta tggcgttatg ggttcccttt
    69121 gcaaccaaca atttttgggc gctcatgatt tttaaagaga tgacccttta tttatttttg
    69181 tatttgggca ttgtcacctt tgtaaatact acgagtagga tgcaaaaact aatttttatg
    69241 tggttggggg tgcacgggtt tcttggaatt aatggaatct tacatcacgg acaaggggtc
    69301 ggaggatggc tgggggacga aaacgacttt ggaatggaaa tgaatgtggc tgttccggtt
    69361 gccttcttta tgtatcaggc cgcaagtagt caacgggcta agttgctgta tatagtccta
    69421 ttaggtctat ttattatgtc ggtagtggcg acggcttcac gaggtgcgtt cctcggatta
    69481 ttggcgattg gagcgtattg ctggttctat tcacctaaaa aaatcatgtc gcttattttg
    69541 gggacatgtc ttgtcggtct tgtgctcctt gcagctcctc aagaatactg ggatcgtatc
    69601 agcaccatta cagatgataa gactatggag gaaggaacgg ctggccaacg catgttcacc
    69661 tggggaatag ggtgggaaat gttcctagca aatccggcct tcggtgtggg gcaaggcaac
    69721 tttccatgga caattggaga ataccttggt ggaagaacat ggcaaaccaa gtcgctagct
    69781 ggtaggcagg ctcattcgct ctattttacg ctgcttcctg agctggggct tgtaggagtg
    69841 atccttttca gctcaatggt atatttcaac tacagggatt tgaggttgaa agagtttcac
    69901 cgacatccat cgcgtaaaat tatcggagga ctgggagaaa gaaaagatac ggagatttca
    69961 cgagccttca tgttcggcaa tgcaatcttg ggcggtatga ttggctacct tgcaaccagt
    70021 acgtttatct ctactctgta ctatccaact ttttggacaa tgatggcact tgcagtgtcg
    70081 cttagaaata caacaatgaa ttcgtcagcg ccaagtaatg aagaggttcg acagttaatc
    70141 cgaccttcaa tttcccggca tgctaaagac attagacata tatagcgatt tctccctctt
    70201 tgcttgtact cctccctctg taaggcaagg gatatctgaa tttcgcacac tgtggctggc
    70261 tcacatggaa ggggttagcc gcgaagcgat gcaaatggaa tgtaaggcta ccctgcttta
    70321 gaaagcgcca tggctgttcc caaaaaagag gtactttata tctggattct tctctctctt
    70381 tcagtgttat gtgctgccat actcggagag atgggattga gattgatggg atacaagggg
    70441 gcaccggaat cattgatagc taatattcgt ccaatcgatg atccaattct caattggcgg
    70501 tatttgcctg gttcagaagt gcagacagga aaaataaaaa gaaaatataa caaatccggt
    70561 tttcgcgatg tcgaccataa cgtggaaaag gctccgggcg tcaaaaggat actcgtcgtt
    70621 ggtgattccg tcacggaagc cggcgatgtt gagtgggaag ctgtcttctc ttcccgactg
    70681 caagctgatt tgggaaggtc ctatgaggtg atcacgctgg ccatgggagg gctcaatacg
    70741 ccgcaagaga tccatctttt tgagaaagaa ggacttgcgt acaagccgga cctcatgatt
    70801 ctgaattttg tattgaatga tgctgatttc tataccgaat ataatgcgac ccagcgttat
    70861 caagcggaaa aagattcagt tgtcggagtt cttggcatct caattaatcc agctttcaag
    70921 agaactctga aatccagtgc actcgtgtat tttttgaaac agcggttcga acatgcaatc
    70981 ggaatgtttc tgggaaaaga ggaaattggc tattttgaaa acctttgggg gaaggccgag
    71041 aatcgtcaaa gggtcacgaa tggatttgac cgattgtggg ttatgagtaa ggactcatct
    71101 tttccggtcc tcgtcgtcat ttggcctatt ctgacagagt tcagtcgcta caaatttgag
    71161 catatccatc aatgggtacg tgaggaggcg gaaaaacgag gattccagac tctggactta
    71221 cttcctcatt tcgcaaaaac ctcatatcga aatctgcagg ttacggcaga ggataacatc
    71281 catccgaacg gtctggggca caagattgga gcggatgctt ttctcgaatg gattcgctga
    71341 agtgagtctc gtcgagagtt ttgtctcaat ttggcccgcg attcttggtg tagttgtgct
    71401 gaggatttac agggctatga agtggtgcag gggaaatgtg aatcgtgagt tctgatattg
    71461 agaaaatacc ggtgctctat ctgtcgagca gtagtgggcc tgggggggct gaacggatgc
    71521 tctgctctct tgctgcttcc attgaccgcg cccgatttag gccaattgtg ggattgttta
    71581 ggcctggctg gttgaaggaa caatgcgagc agcaaggaat agaaactcat gtattcccaa
    71641 ctggaggaat gttgcatggc gcttggatta gtggctgcac gaggctcgtg cgcgaggaac
    71701 gcgtggcact cattcatgct cacgagttcg atgcgaatgt ccatgggact cttgtcgctg
    71761 ctatcacagg tgtgccaatt gtcgccactg ttcacggaaa gcactattat tggacaaagt
    71821 ttcgcagaag agccgcatat cgtttcgtta gccgatttgc cacaatggtt gcggtctccc
    71881 agaatcttaa ggattttatc ggacaaaaag ttgggatcga tcaagcaaga ataagagtca
    71941 tctataatgg agtgaagcct ctgcctgaat attttgctgg aaacattctc ggctgcaggg
    72001 ttgaattagg tatcccagaa ggcgatcaga ttgtcggggt cgtcggaagc ctgtatcccg
    72061 taaagggaca ccggttctta gttgaggcaa ttccccatgt cttagcgaaa tttcaccata
    72121 ccacgttctt gt
//
LOCUS       DAORRQ010000018        68817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_22_length_68817_cov_144.660321, whole genome shotgun sequence.
ACCESSION   DAORRQ010000018 DAORRQ010000000
VERSION     DAORRQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 68817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22_length_68817_cov_144.660321"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK224_07705"
     CDS             <1..212
                     /locus_tag="PK224_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsI"
                     /protein_id="HRB15659.1"
                     /translation="VELLRFIEVLEQTLGMKAQKNFLPLQAGDVPATYADVADLMRDT
                     GFKPATSIETGIARFVEWYRAYYKV"
     gene            690..1076
                     /locus_tag="PK224_07710"
     CDS             690..1076
                     /locus_tag="PK224_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15660.1"
                     /translation="MIWSLLAAGWLMLAGQGLALAEAPSTETATWSELRSQSSNALSQ
                     APATSERQVDIFRQTPMLSGRLQVSEQTLIPYVGAGFSGGYVTERDRALGPMPGLPQQ
                     NLLGDSLGLGKGMMPNEFQMGIRIPF"
     gene            complement(1111..1803)
                     /locus_tag="PK224_07715"
     CDS             complement(1111..1803)
                     /locus_tag="PK224_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRB15661.1"
                     /translation="MFDFNGVIADDETPHLLCFQQALAEAGLSLSREEYYGTYLGMDE
                     RTCATALLERRDGICDPTIHAGIIERKAGLFRAHTALHKPALFPWVTEFVQRASGAYR
                     LAVASGGRREQILAALTGTPIEQAFELIVSADDCAVGKPDPAIYEYTLRLLNARRPRP
                     PLLRANECLVIEDSRAGVLAGLKAGMRVLAIATTYPASQLAEAHLVLPSLNGIDPAGL
                     ARRLFQPLDQKG"
     gene            1983..4007
                     /locus_tag="PK224_07720"
     CDS             1983..4007
                     /locus_tag="PK224_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRB15662.1"
                     /translation="MSDYAVLGDLLVIYAVSTAVVFVFHQFRLPSIAGFLVAGALIGP
                     HGLHLIPDVSQVQVLAEIGIVLLLFTIGMEFSSAHFAAARRILMVAAPVQTGGVLVLA
                     LLGALAVGLSYQQGIFWGFLLSLSSTAIVLKALSEQGESDSFHGRATVAILIFQDLAV
                     VPMMLVTPILGTPSGSAMGTVLMTLVKAAIVVGLIVAAAWYLVPRLLRHIVRSRSREL
                     FLLSIIVLCLGIAWLTSLGGLSLALGAFIAGLVISESEYSHQALAEVLPFRDSFNSLF
                     FVSIGVLMDIRVVLDHPFIVLGLLLAVIVGKLVTGSGAMVAAGAPPRSSVLVGVALAQ
                     VGEFSFILAQQGQDAGLFTGDQYQLFLAVSVLTMVVTPFLMQWSPDLGRRIEAMQRVR
                     GWMPTRTAAHVEQLESSQIRIKDHVIIVGYGLNGRNLARVLSETEIPHVALDLDGETV
                     RREARHGVPIYYGDGSNANVLRHMKIDDAKVLVVALSDPFTARRTVKVAKGLNPKLHI
                     VVRTRYLRELEELHQLGADDVVPEEFETSIEIFALVLRTYSLPQEFVARKAEQIRREG
                     YALLRRSEMPELAHHLRGGTLTDVEVETCRIDDEAPAVGKSLAELSIRPRTGASVIAW
                     TRSQVTQSNPSETVRLQAGDVVTLLGSRDQIRRAMALLNEPNHGTSHQLK"
     gene            complement(4017..5105)
                     /locus_tag="PK224_07725"
     CDS             complement(4017..5105)
                     /locus_tag="PK224_07725"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRB15663.1"
                     /translation="MSTDLITELKPTKSAPLYGFWYPATTSTALAVGQMQTQVMLGQP
                     ILVCRDRQGKVAAMRDICPHRGMPLSFGHFDGERVECAYHGWQFDTGGRCRHIPALVE
                     GSPLQPEKIGITSYPCQDQDGYVWVFLPDPQQPKQPVPELPRLPLPSEPYRMIQISTI
                     LDCTIDDGIVGLMDPAHGPFVHQSSWWRTKASMHDKAKTFEPIPNGFRMVPHAPSKNS
                     GPYKLLNRLYGGPLTTTIDFVLPNQRFEFVQCGSMYVSSRATVTPVGEQQCRIDFAAA
                     WNILPWLPFAKPLFRYFANIFMKQDKQAMERQAVGLKYKPALMLIDDADTPAKWYYKL
                     KAAHLAAVQTGRPLDHPLKDRVTLRWRS"
     gene            5350..5880
                     /locus_tag="PK224_07730"
     CDS             5350..5880
                     /locus_tag="PK224_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRB15664.1"
                     /translation="MTTAQIEPTAALAELREAKPDRVTIVLLSGDLDKAMAAFIIATG
                     AAAMGMQVTVFFTFWGLNTIRKKGASSSAPDWLRRMFGLLNKGGAETLPLSRFHFWGL
                     GTKMMQVVMKQNRMPGVPELMETALELGVRFIACTTTMGLMGITKDTLIDGIDQFAGV
                     TTYLAEAKQGSVNLFI"
     gene            5904..6146
                     /locus_tag="PK224_07735"
     CDS             5904..6146
                     /locus_tag="PK224_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRB15665.1"
                     /translation="MQADVKLDTLGYFCPMPIILTSKKIKELTTGQVLEVVSDDEGIK
                     KDMPAWCETTGHEMVGLEEEQSASKRIYKAFVKKTK"
     gene            6286..7848
                     /gene="ftcD"
                     /locus_tag="PK224_07740"
     CDS             6286..7848
                     /gene="ftcD"
                     /locus_tag="PK224_07740"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRB15666.1"
                     /translation="MSQVVECVPNFSEGRNSEIVDALAGVVRSVPGVVLLDETKDPDH
                     HRAVVTFAGRPYAVAEVAYQMARMASQLIDLRNHHGEHPRVGATDVMPFVPIRGVSMQ
                     DCVQLARMVGQRIGNELKIPVFLYEQAATRPERKQLEWIRKGGLKGLADRMASDPAWV
                     PDFGPKLLHQTAGATVVGARWPLIAFNVNLKSRDLSVARAIAKLVRQSSGGLPYVKAI
                     GVELKSQQLVQVSMNLTNHEETPLHVVFAAVQREAAARGVEVAGTEIVGLVPEQALME
                     TAQQALCLNQFDGRQVLESRLDRPESREAIGRMAASQPVKEQSPLPAKVGAPDGKMPE
                     LQGQTGGSVGAQSAAFAAGLGMMVAKLTRARAIETRLSEIRTRLHELGQADRDAYGRV
                     LQARKLPITHPDRPLQLSSNLLGAIETPLEIVKLSCELIPLLRGLLTQTKPELQPDLK
                     MGLRLADAVIDGCLGMVEENMKVQPNQLLIASIRERFSVAEQMLVDAKSLCYTPPFDS
                     WAQNMLNILKLR"
     gene            7872..8066
                     /gene="rpsU"
                     /locus_tag="PK224_07745"
     CDS             7872..8066
                     /gene="rpsU"
                     /locus_tag="PK224_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRB15667.1"
                     /translation="MEIKVFNNNVEKALKVAKKKLAGEGLFRELKRRRYYEKPSVRKK
                     AKEREAQRRRQKWLAKRRPE"
     gene            8138..8797
                     /gene="nth"
                     /locus_tag="PK224_07750"
     CDS             8138..8797
                     /gene="nth"
                     /locus_tag="PK224_07750"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRB15668.1"
                     /translation="MQAQEIHAAIRTVKREIARWPDPVVGVVARQSGRDPFLVLISCL
                     LSLRTKDKTTAEASERLFALASTPATMQTLTTPVIERAIYPVGFYRTKAKQIQQICAQ
                     LRERYQGRVPDKIDELLTLPGVGRKTANLVVTVGYEKPGICVDIHVHRISNRWGYVKT
                     KSPDETETALREKLPRKYWITFNDLLVPYGQHLCQPVSPLCSQCKIAAYCDRVGVTKS
                     R"
     gene            complement(8861..9280)
                     /locus_tag="PK224_07755"
     CDS             complement(8861..9280)
                     /locus_tag="PK224_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15669.1"
                     /translation="MSGKPKFVIFAHNATYDKLHQVATLGLTAAAMGKDVIVVLLFWT
                     IKKLAEGKIDEIDFPPEYAASAEQVARLLKEKKVPKISEMFQEARLVGHFRLIACSAG
                     LEYMGVEAGAVERNVDEVLGLPSILSLTAQAETKLFI"
     gene            complement(9286..9681)
                     /locus_tag="PK224_07760"
     CDS             complement(9286..9681)
                     /locus_tag="PK224_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15670.1"
                     /translation="MPPMRLNSARVTRACLQASGLAVLGFALSLCPVALSSHAAWAAD
                     GTTESELQIDITKIGLGKEVVITQGKKEWFMRVEVTPENSVVVRQEKEGERYLLDESE
                     MHDRAMTPAEVDVAIADFVNSVKTRQKVK"
     gene            9775..10206
                     /locus_tag="PK224_07765"
     CDS             9775..10206
                     /locus_tag="PK224_07765"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HRB15671.1"
                     /translation="MISELLTQIESYVPANVLIWFAVSSVFMFVGTLIAIPIILMRLP
                     ADYFDVRTPRPWMENHHPILRVIGHVVKNVVGAIFLFAGFLMLFLPGQGVLTMLIGLS
                     LIEFPGKRRVEAKIVGQSTVLSTINAMRAKFDKPPLIIAPD"
     gene            10265..12910
                     /gene="polA"
                     /locus_tag="PK224_07770"
     CDS             10265..12910
                     /gene="polA"
                     /locus_tag="PK224_07770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRB15672.1"
                     /translation="MPTLYLIDGSAYIYRAFFALPPLANSKGLQTNAVYGFTTMLLKV
                     LRDHRPDYVAVVFDEKGPTHRHEAFKDYKAQRPPMPQGMSAQIPYIHRVVEALSLPVI
                     RQAGYEADDLIGTLARKGEVEGLDVVIVTSDKDMFQLLTPKTRIYDPVKDKWFGEADS
                     QARFGVEPARVVEIMGLMGDTSDNIPGVKGIGEKTAVKLITQFGTIEELLNRLEEVTP
                     TKTKNLLREQGEQARMSKQLATIQLDCPLEFVPAHFQAKAPQTDTLVSLLRELEFMTL
                     AKAFQGETPEHNRLGTDVEQIHDAAAADAFLKEQPAVAMLGIACVLTGEPGVRADIQG
                     CALGRSDGHAAFVQGEARDWPRPLIECLHDGSKPKAVQDLKPLLLALHRQGIDMPGPY
                     FDTMVADYLLNPNRRAHTLEAIAMDLLSYQLGAGVSEDTGKGPQSLFDVDENLVRRTG
                     EAAAVTAKVAPMLCERLREQGSLPLFQDVEMPLVPVLAEIERNGFLLDVEGLQTLSKE
                     LERELEQMVGSIYRLAGGEFNIGSPKQLATVLFETLGLKPLRKTKTGYSTDEDTLTQL
                     ASQHDLPAQILNYRTLTKLKSTYVDALPQLINPETKRLHTSLNQTVAATGRLSSTDPN
                     LQNIPVKGDYGLRIREAFIAPPGHQLLCADYSQVEPRILAHLSQDPRLLQVFEKGEDI
                     HMATAMEIFNLPAGEVTRDMRRAAKSVVFGIVYGISPFGLASNIGVPQADAKKYIETF
                     FEKFAAVRALMDRNIDDGKTKGYTTTILGRRRPIPELQGGDPSQRGVGERMAVNSPIQ
                     GSAADLIKVAMIKVHQRLQNELPRCKMILQVHDELIFEVPDQELEPAKQLVKAEMEAT
                     GAALGLSVPLKVDLGVGLNWRVAHP"
     gene            13041..13427
                     /locus_tag="PK224_07775"
     CDS             13041..13427
                     /locus_tag="PK224_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRB15673.1"
                     /translation="MLPRKRTLTIPEPEFQALVQEALDGLPDEYAKLITNVAVVVEEE
                     PPPEVLADLEMEEDEDLLGLYQGLSLDKESFFQAGGQLPARISIYRGPILRLCRTKKE
                     VVQEVRDTVVHEIGHHFGFDDDEMPY"
     gene            13542..14360
                     /locus_tag="PK224_07780"
     CDS             13542..14360
                     /locus_tag="PK224_07780"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRB15674.1"
                     /translation="MKKLHVSQSLVEVESLKDLLEGAGIACMVRNQQSSSLAGSVPFV
                     EVFPELWVLNDQDYDIGKELLAGQASTAMPGSDWTCAVCGECHATEFVTCWKCGVEKG
                     VEGSQKVAIQSEASPSGASLGFGVLLGVCLGVAATLCMYWLWRYSSMTSTAYDRNADG
                     KDDVVYEYKGQFPWAAKLDNDFDGYFETALFYNQAGEVIRTEIDRGRAGRPDLIEYNA
                     LGRLDSVEFIDRTTGRVKKRMYYKLDAKVREEIDEDGDGKFERVIEFDEFENPK"
     gene            complement(14486..15013)
                     /locus_tag="PK224_07785"
     CDS             complement(14486..15013)
                     /locus_tag="PK224_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRB15675.1"
                     /translation="MWYGEPLSDARTPLADFFRILLTLAGGWVYSALVEPRATTDIDL
                     LILLDPPSREQIQALVSSLFDSTIIHPNPMALQGISIWRIVGIRSGQEVVIDFLLADS
                     AYLRTALERRHTVPFGALQVPMLSIEDLVILKMVAGRLQDRADLEKIRTRQTDLRVDW
                     TYVDRWKTMLGLAER"
     gene            complement(15204..15407)
                     /locus_tag="PK224_07790"
     CDS             complement(15204..15407)
                     /locus_tag="PK224_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15676.1"
                     /translation="MSPSSPSPDDLRIADNLRRTIELTELGLALRQAVIEQRNVPGAG
                     EGIAQVMHEIRLAKEQAWQQSQS"
     gene            complement(15572..16825)
                     /locus_tag="PK224_07795"
     CDS             complement(15572..16825)
                     /locus_tag="PK224_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15677.1"
                     /translation="MPIENNFQHDELSRKNPGDRLTYADWTTPPDADSPAWQEAMSQL
                     GQKLSQAGVRLIMFLHGAIPGTDVFGLGRLDEVGGLKRGYSRGISGLDSLLSFMREGT
                     NGITPLPGGMKPPLSNDEATKTLLDSQIGDAGNFTTTTVEHCRKALNQKLATPITCIR
                     ELWSSEHHHLGRAMAACRLLDRLRALVIEQHLGAGDRILIQAHGQAGLVLALASNLLC
                     PSPITGRKTLFDLLLAANPQGQDAQAIQRIDPLLTNGTLLNGGALDIVTFGTPVRYGW
                     DPSGIGKLLHVVNHRNLRTDGKTWLSKMELPQISVEMPIAWGGDYVQELAVAGSDALP
                     AENAKAANKAVWELLEPYDGFERWVECARRAGRFPSEGQCLLVDYKDCTSSTNVRDHY
                     YGHAAYTRTNAMLFNFNQIVGRLYP"
     gene            complement(16898..17455)
                     /locus_tag="PK224_07800"
     CDS             complement(16898..17455)
                     /locus_tag="PK224_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15678.1"
                     /translation="MSLTAGPIRTGWWPLCALLIASPATAADLTPPAVRDHADQTFQH
                     LHMQGRAESPTSAAQARPAQATSSSEELLTGQGQGDLGKGKLVCQRVSELAARADIAK
                     QIRVLVKEHATDRLRERTGREVEQDIEVVREEIVQEYLQGVRIVERHTDEEAKTCSAT
                     AVMPKPRLSAPASMESNGRESAAHR"
     gene            complement(17455..18636)
                     /locus_tag="PK224_07805"
     CDS             complement(17455..18636)
                     /locus_tag="PK224_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15679.1"
                     /translation="MPQNRFDSGPRFIPHTSPRARSIHLFIGLLSIALSLLLTCPSPS
                     QAEVRVVTGTGEHRMNDHETKTDGIRLATEQAKKQALEQVASYLESVTVVRDLDVTLD
                     EIRSYTAGMVLVLDQHVTTALNGEFVVVRVELTAQVNTDEVAQAIATLRQNEDAREQL
                     LALRRDVESLQQELDAANDTLAVATSPEQVQELSHERNELLNRAESDAMVAQAWTDWV
                     LLTPFAQPSPSAGLAQIHALLGLASRLNPDNPHLAIAQQTVGTKAPPAPPQPRRPPVP
                     HTVPFLPGYAVVPQQSAQPGAIQVPAQPGAPLPQGSLGTMPTYRRVPPPSGRPAARTL
                     HTYRSGVDQATPAAEQPSINAPRGTPPAIIYQVPKKPVPIPTTPSQQIPGNGTHQGGT
                     R"
     gene            18822..19871
                     /locus_tag="PK224_07810"
     CDS             18822..19871
                     /locus_tag="PK224_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRB15680.1"
                     /translation="MSRIRSILTVTAALCMVVGLGLALVAPSSQSVAFAARGGGEVAT
                     PVQAGITEHVILFVLEGLGQESLKSGAMPVLSSLVKDGAVTWSATAVAPARRLPTMAS
                     LVTGMPVAKHGITWNVFEFSRGYPRAPTVFDYLDLSGGRDSAIFYMDESLYQLAKPEP
                     YTDYQMCGPLRAECNPDRLVGYVRDYFKKATSGSGYGHAIPALPHLLVVHLPAPGRVG
                     ETQGWKSVAYKDALKAVDKAMGTVLDLYREHGLIKRTTVFATSLSAFGETQFSAGEVS
                     GEQAGNVPVVPWIASGVGIKAGHSIRQPVSIIDTGATVMRALGLTTYTEWESHPVEEI
                     FKTAFAAAPVSPLLQ"
     gene            19879..20370
                     /locus_tag="PK224_07815"
     CDS             19879..20370
                     /locus_tag="PK224_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15681.1"
                     /translation="MRSSSHKTFAGRQVFFWIVAVTCVLVLGPSERLLAAGPPPAYGK
                     EHTITIDPTPLVPQSWWQVPGVTPPLWVLDPETSDAYRTTETRELKLKPGQYKFISFT
                     FDFPFTVSLDGKLEYSKSLDQCVEGRGTQTLVVRCKRTYPHGGQRDAYYGNSEVGDGT
                     GTR"
     gene            20351..21721
                     /locus_tag="PK224_07820"
     CDS             20351..21721
                     /locus_tag="PK224_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB15682.1"
                     /translation="MAQALDDLLDSLEDADDATREEAAKALAELANPATLDALIGACG
                     DEFWSVRTRAGWGVAKIGGPKAIEALIVLFNDPIMEVRNEAVAAVVSLGAGQLDRLLA
                     AMKDERWRVREHAAKACGDLHDSGAVDALIFACRDRDGAVKSAAAEALGKIGDPKAIP
                     ALVKLFRDSSKIVRETAGIALVAIGQPSVDLLLETLKDKDFVVRCHAARALGGMTTDY
                     QIGRSWVREPRVVDALIEALKDPDRAVREDATIALGMIGDPRAIDGLLEAMKDGAVKR
                     HAIASLGMIGDPRALPAVLAALKGKGVRQDGTPTPGCIVSEDAFIKEAAATALGHFRD
                     PRVIPDLIMLLKDGVLREKAAAALVLIGDSAIEPLISFLYDPKASEVEAEGERVLSYA
                     SVRLTAKDSLRLLVVETLEHLGWTPPDEETSVDSSQADNLRVDRPLGDIGRFGPSGDF
                     AKGSVR"
     gene            21909..22991
                     /locus_tag="PK224_07825"
     CDS             21909..22991
                     /locus_tag="PK224_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB15683.1"
                     /translation="MADTVSEQIAALSDEDWAIREEAATLLGSLNDARAVLPLTKLLR
                     DADRAVREAAIGSLSALGSASVPALAGCLADPALQVQEAASAILASIADARVFRPLVE
                     ALGSRDWIVRMHAAKGLGRIGDPEAVSVLMPLLQDKVKAVREEASSALAAIGAAAVLG
                     LIQALEHDDWLVRLHAVEALGKLKSPDSVDPLLRALFNERDSAIREDVVRALGAIRDA
                     RAVDYLVTVMKEPGLRLLAVEALGHIGDPRAVPLLRRVVEGVPLGEPRDTATPCADGW
                     TDEMATMGMAARALGMIADGVAIPSLIVALRNTITRSEAAAALAKFGPAVIPSLLPML
                     AKEQDENIRYHVRETLTAVGWRPGRV"
     gene            23087..24187
                     /locus_tag="PK224_07830"
     CDS             23087..24187
                     /locus_tag="PK224_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB15684.1"
                     /translation="MSKESIDTLVSELTHEEEWRRMRATAACLAGGPRAVQALTQALE
                     SGDVALRVEAAAMLSRIKDPAAGLSLVGLLRDSEEAVRQAAFSALEQMAGNLDDETAA
                     GLVRNLHESPDEDVRSRVRQLLGVIPNAITPLCEMLTHPEVDAQTTAATILEHLLDPR
                     SADGLIDAMSSPAVRDTAVRTLKKLGAIRDRIDATFNALRDVEGASEREEARMSTVMD
                     LLGIGRPAVEILIEYLEDDDWVVREAAADLLGKIGDVRAVEPLMKRLQLDKDTGVKEY
                     AVKSLGLIGDPRPAQLYIEAIPIRPLRVMAIEALAKIKDVEVLRPYNELFNRLRTDRD
                     GIVSYSAGLIADKLAALEGEQPVGQEESDDHE"
     gene            24180..24941
                     /locus_tag="PK224_07835"
     CDS             24180..24941
                     /locus_tag="PK224_07835"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB15685.1"
                     /translation="MSDAERIAQLISALRDDNEALRDHAMASLGQMGIEAVPQLIGLM
                     ADEDVVIREAAATAVVRIGPVAFDQMLEALSDDEWAIREQAANVLGRFRDPRAVDPLM
                     VALKDKDGAVRTAAVWALERIGDSRATPGLIEALGDGTVREDVARVLKKIGDTRAVDA
                     LIGGLLGPNWMVRRHAAEALGKIGDPRSADALIQSLQDEDWLVRRNAAESLARLGAKQ
                     AIEPLLPLLEDENTMVQETVEGVLASLGWKQATSS"
     gene            24962..25672
                     /locus_tag="PK224_07840"
     CDS             24962..25672
                     /locus_tag="PK224_07840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB15686.1"
                     /translation="MAEEAPVKLIQIGPKGGTKKDGFNLVTERVVAVNPEAKQLEVEL
                     LAYDGKTVVLDVGDEALEDFLKIKPGDGATIRVVEEGGKRIAKSFRIRAKDPNAAKAD
                     AMLIDLKDSHWLNRKYAAEVLGELKDPRAVLPLVEALTDEVGDVRQRAYDSLIKIGGI
                     AVASLVPLLASEEDDVRQSATEIIRKIGKPAVEPLATALADADDRLKTRIMKVLDRMG
                     YKPKAKEGAQAEPAKLLS"
     gene            complement(25694..27013)
                     /locus_tag="PK224_07845"
     CDS             complement(25694..27013)
                     /locus_tag="PK224_07845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRB15687.1"
                     /translation="MHISVIGTGYVGLVTGACFAEFGVNVTCMDTDARRIARLEKGEV
                     PFFEPGITELVAKGIKEDRLHFTTDVAKAVDKALVIFIAVGTPPKSDGSADLSYVEEV
                     GRGIAKNMTGYKVIVTKSTVPVGTGEKLREVIKANQTGRFRFDIVSNPEFLREGSAIE
                     DFMRPNRVVIGADSEQAVAIMKDLYRPLYLLETPIVVTDIPTAEMIKYASNAFLAVKI
                     SFINEIATVCEKVGADVQMVSKGMGLDNRIGNKFLHAGPGFGGSCFPKDLAALVQTGE
                     RVGYPFQIAGAAAKVNYEQHLRMVEKVKDACGGVKGKTLGVLGLSFKPNTNDMREAPS
                     LTILSELMKEGATIRAYDPASMEESMKLLPGMVPCQDTYDVAEGADGLIIMTEWNQFR
                     NLDFDRLKKSMRQPLLLDLRNTYESDRVVGFGFRHVSVGRPTKNPAA"
     gene            27204..27614
                     /locus_tag="PK224_07850"
     CDS             27204..27614
                     /locus_tag="PK224_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB15688.1"
                     /translation="MAGSGGPIQRPLAMMMRPITRTVHPDDTLLAVARQLRDARVGAM
                     LVADHGDYVGIVSEADLVRKAMASGADAEQVMARSVMSTPVMTIDIAQSAHEASDVMA
                     ERGIRHLVITEEGRMVGMISVRDLLRYFKNWGTL"
     gene            27708..29252
                     /locus_tag="PK224_07855"
     CDS             27708..29252
                     /locus_tag="PK224_07855"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRB15689.1"
                     /translation="MPRLFLLITALVTGAIVMALEILGSRLLAPVFGNSLFVWGALIG
                     IILAAMSSGYAFGGWASDRYRVAAVLAWLLLGSGAWTLLMSWMGQVAIFKVAKLVEDP
                     RWGPSLAACVLLAPPAFGLSGVMPALLRLAVVDMGYLGRHTGSMIALSTVGSLAGTWG
                     TAFFLLSWLGTQTLVASLGVLQLLLGLLWLQQGSGKVTKVTGLLLAGLLILSWQLFGQ
                     AAPVAGLVYQEDSPYQQVRVRDDDLFRYLVLDRTWHAVMWRSDPATLFLPYSQLMVAA
                     VALTPDPKRGLILGHGGGSLAKWLAQVWPELELDVVEFDPVVVRMAQEYFEYHPPANH
                     HVFVKDARVFVRDTGVKYDVIWVDAFARHLIPFHLTTVEFFSELRSRLNPNGVLALNL
                     ASSGEGGDLQRASAVVQTLKTAFPTLESFGVKGPWRAHQTTAENLIFFGGGPIDTMPY
                     DEVVKRIQSHVEARRLPSEATALLAARRTQPWSPGLTLTDDYTPYDLLIGSHAVEMSP
                     EGKALP"
     gene            29528..30007
                     /locus_tag="PK224_07860"
     CDS             29528..30007
                     /locus_tag="PK224_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15690.1"
                     /translation="MGKTMVAVFFGLVMLVAGASGAYALQAGGVEIGDLDTGSVVGQP
                     FKELVVDAELYAVEIGNMKAWYPPVTVIDFKVRPGRPVVLKVTNNSTAEHGFQMTDAA
                     NSGSPFVMKAEVVLKPGETKYIGVPTSDMFYATQGNTLTYRCHLHPAHVGGKILMIK"
     gene            complement(30114..30449)
                     /locus_tag="PK224_07865"
     CDS             complement(30114..30449)
                     /locus_tag="PK224_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15691.1"
                     /translation="MTRVSRLILFASLGLALLPVQTMADTGDVGTIIESFVTKQFPDA
                     ANHFWIVNDTQWDGDEMIVDMNAIVTERRQDVPTASRFLLLIVDGRLRGIQNIPLDAE
                     AECQKEQEA"
     gene            complement(30654..31001)
                     /locus_tag="PK224_07870"
     CDS             complement(30654..31001)
                     /locus_tag="PK224_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRB15692.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAEGKGNHVGDGQGV
                     GDNIYVITHDRCTECVGHFDEPQCAAVCPVDNCCIADPVYPETTEVLLDKAKTLNPDK
                     EIDPAKVWSGVRN"
     gene            31249..31746
                     /locus_tag="PK224_07875"
     CDS             31249..31746
                     /locus_tag="PK224_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRB15693.1"
                     /translation="MVRRASAAESEQRRKKIVTFVLLVILLVSVSLFLGGPKESELII
                     VEFPNGKTMETEVASTPEKLLFGLAFREGLPADTGMLYIFESTGLHRVQTRQFRIAVD
                     MVWIDESHHVVHILEQVPPCEKDPCPLYGPPPEPVRYLIEAEAGSVQRAGITTGMELK
                     FTLRM"
     gene            31763..32080
                     /locus_tag="PK224_07880"
     CDS             31763..32080
                     /locus_tag="PK224_07880"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRB15694.1"
                     /translation="MEGFQCVAKVEEIPPGQVKVVKVNERGIAVFNIDGQFHAIYNSC
                     PHEGGPLNKGRVKGHVVSCPWHDLAFDVRNGQGTDGGGYCVGSYDVRVEDGQVFVGGR
                     RKV"
     gene            32119..32451
                     /locus_tag="PK224_07885"
     CDS             32119..32451
                     /locus_tag="PK224_07885"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRB15695.1"
                     /translation="MSPLSKAVPTGAEQEPGAKTLKARVGASVHIRLWEDRTRGELWV
                     PSYDATVMPLVEDDFLRTASNNAVDAGQRTFEFRPVAAGTHRLVFEKRMGWKFTAEDR
                     QIFYVTVS"
     gene            32479..33318
                     /gene="dat"
                     /locus_tag="PK224_07890"
     CDS             32479..33318
                     /gene="dat"
                     /locus_tag="PK224_07890"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HRB15696.1"
                     /translation="MPDIAYVNGRFGPLADAVVSIEDRGFQFGDGVYEVIRTYRGQPF
                     AIEEHLVRLERSAQALQLAIGQTRAQWSSLIREGLRLSQFPEAKIYLQITRGQAPRDH
                     PFPVSSAPTTVLTFRELHPLDVSVRNAGVQAMSVEDIRWGRCDIKSVNLLANVLARQR
                     AKEAGVFEAILVREGTVTEGSVSNVMVVRNGAVQTAPEGRRILSGVTRAIVLDLARKE
                     GIPVAESFVTREELLAASEVFLTGTTVEVLPVVRVDGRAIGGGSPGPVSQLLSRRFEA
                     LIG"
     gene            33408..33926
                     /gene="rimP"
                     /locus_tag="PK224_07895"
     CDS             33408..33926
                     /gene="rimP"
                     /locus_tag="PK224_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRB15697.1"
                     /translation="MSDAGAVSAGKPSVKRAEALNLRIQEIAAPILQSHGLELVEAVC
                     VGQGPRTVIRVFIDKPGGITLTDCEQAHRSLSPALDVIDPFPHAYTLEISSPGLDRPL
                     RSVQDYRRLIGKPVTLKLCEPIQAQWRLVGAVVEVDDRGVTLAIQQKKTTETIRVEFD
                     QIALARRKVEFS"
     gene            33969..35108
                     /gene="nusA"
                     /locus_tag="PK224_07900"
     CDS             33969..35108
                     /gene="nusA"
                     /locus_tag="PK224_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRB15698.1"
                     /translation="MNRELIAVIDEIGRQKGIDKARVIGAIESALQTAAKKRFGQAEN
                     IQVEIDPKTGEISVVSKKIIVDTVANPKAEISLKEAREYDEGAEVGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNFLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKALLLEVRRTPKDVQVILSRSHPQFVAKMFELEVPEVGEK
                     IVEIKSIVREPGDRTKIAVSSRDKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     QDPRVFIAEALNPATIEKVGIDEEKKSALVVVADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKAERDRDIKAALAEETEAQRQQDEARAAAQQAE"
     gene            35172..37769
                     /gene="infB"
                     /locus_tag="PK224_07905"
     CDS             35172..37769
                     /gene="infB"
                     /locus_tag="PK224_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRB15699.1"
                     /translation="MRVYELAKQLGMENRELIPELKRLGIPVASHSSALDDDSVRVVL
                     EKLSSKARGGEAVSGGHEAKKMVRSSKEPGASHERGHAAAHEEPPKPDKKRILIKKKR
                     EEGAEDVAASLAAAEAAFAPVVPAGTEASVVAPAAGPDAEAIEAAPVEPAPPEELVSP
                     PPVVTVSEEPLVKPVQVPQVTAPPLDALATAKKKSAATEAMESEAAAREKLKKAKKAP
                     RTREEDEAKFKNDATRWGDLRAIPVQRREDRSKHIHHASPTEVTKPRKKSVKLSAGVS
                     VKEFAELIGQRPADVVRKLMEMGQMVTFNQSINLEAASLIAEEYGTRVEVSTEKVGEA
                     LLEEAAQSSGEEHAVLRPPVVTIMGHVDHGKTSLLDAIRLTKVAEGEAGGITQHIGAY
                     IVGVRGKQVTFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTVEAIHHAKAA
                     GVPLIVAINKVDKPGANIDRVKNALTEHGLVPEPWGGDTIMVEVSAKQRTGLDQLLEM
                     ILLQAEVLELKADPSRMAKGLVIEAKLDRGRGPIATVLVQSGTLHVGDAFVVGNFSGR
                     VRALVTDTGSKTTEAGPSVPVEVIGLPGVPSAGDLFTIVKDERVAREIAQERAMKQRA
                     ADLAGPAKVSLDDLFAKIQEGNVKELPIVIKADVQGSAEALAAAVEKMPAGAVKLRVM
                     HTGVGGITETDILLAAASKAIVIGFNIRPEPKAAALAEREGVDVRLYSIIYDALNDIR
                     AAMEGLLEPTLKERVLGRAEVRQMFTIPKAGLVAGCYVVDGVISRASVGVRVIRDSVV
                     AYEGKLGSLRRFKDDVREVQQGYECGITVENFNDLKAGDIIEAYVVDKIAAKLEPANR
                     GTSPQSHRA"
     gene            37766..38047
                     /locus_tag="PK224_07910"
     CDS             37766..38047
                     /locus_tag="PK224_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRB15700.1"
                     /translation="MTVGLCTVELFIPDGHSLKEKRQVLQSLKTRLRDKFNLSVAEVG
                     DQDLWQKAILGMACVANESAHVNQVLDQAVNLIQSVPVIQLVRSRIELL"
     gene            38057..38455
                     /gene="rbfA"
                     /locus_tag="PK224_07915"
     CDS             38057..38455
                     /gene="rbfA"
                     /locus_tag="PK224_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRB15701.1"
                     /translation="MTKSTYSRADRVADQIRMEVADILMRKIKDPRVRSVTVTDVELT
                     KDLRIARVFVTTMEQNKDERLVFDGLAKASGFVRAELGRRLALRYLPELIFMKDISGP
                     RADRVLELLDGLHRDQSHEEMLAEPPHTDV"
     gene            38459..39490
                     /gene="truB"
                     /locus_tag="PK224_07920"
     CDS             38459..39490
                     /gene="truB"
                     /locus_tag="PK224_07920"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRB15702.1"
                     /translation="MTMIASMEPRATTVLQDGVLNVRKEAGWTSHDVVARIRGKLRGM
                     KLGHAGTLDPAATGVLPLLVGRGTRIAEYLLEWDKTYLAGLRLGETTDTQDATGTVLL
                     RSPIESLSEARIREVVAGFEGRIQQLPPMYSAVKVGGVPLYKAARAGREVSRQVREVT
                     VFRLDIVDVQIPEVTLRVVCSKGTYIRTLCADIGDKLGVGGHMATLVRERVGPLTLEQ
                     ALTVDEVESRLEQGTLAASMLTLDQALVGLPVCIVGAATAGRVLHGMPVPASEVLAWH
                     GLSSPLSEGANRAIGIKDGAGRLLAIGTMPDGMNAEAPGANAQSIAVSKVLVTEESQG
                     GSIANSIEE"
     gene            39497..39766
                     /gene="rpsO"
                     /locus_tag="PK224_07925"
     CDS             39497..39766
                     /gene="rpsO"
                     /locus_tag="PK224_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRB15703.1"
                     /translation="MALVKEVKTELVKGFQQHDKDTGSPEVQIAILTNRITYLTEHFK
                     LHKKDHHSRRGLLTLVGRRRRLLDYLRKVEEARYRAILERLGIRK"
     gene            39963..42080
                     /gene="pnp"
                     /locus_tag="PK224_07930"
     CDS             39963..42080
                     /gene="pnp"
                     /locus_tag="PK224_07930"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRB15704.1"
                     /translation="MKHVVDIELAGRRLTLETGRIAKQADGAIWATYGDTVVLATAVA
                     SQNAKPGVDFLPLTVDYQERTYAAGKIPGGYFKREGRPSEREVLTSRLIDRPLRPLFP
                     EGYYFETQVIASVLSADKTGVSDVIGIIAASAALAVSSIPFNGPIAGVKIGRVNGQLV
                     VNPDLETLETSDLHLVVAGTADAVMMVEAGANELPEATMLEAIELAHSEIKKIVAKIE
                     ELRKLAGKPKRTVVQEPIDAALTEQVRALVAGPIREAILIPNKSARQERLDQVLAETV
                     AKLKSDEPNRDRHVKIIFHGLEYTEVRNMILEKRVRADGRGPADIRPITCEVGVLPRA
                     HGSAVFTRGETQSLAVVTLGTTDDEQRIDALEGEYMRTFMLHYNFPPFSVGEARPLRS
                     PGRREVGHGALAERALKSILPGKDKFPYTVRIVSEILESNGSSSMATVCGGTLALLDA
                     GVPIKEPVAGIAMGLIKEGNQVLVLSDILGLEDHLGDMDFKVTGTKNGVTALQMDIKI
                     GGITSELMREALAQAKAGRLHILGCMAQALTEPRTKLSAFAPRIFPMKIKQDKIRDVI
                     GPGGKMIRSIIAETGVKINVEDTGDVTIASSDEASAQKAIDMIKRLTEEVEVGKIYLG
                     TVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKAVTDEVSEGDQVMVKVLEIDKQGKI
                     RLSRKEAMPAPAGSPATEPTPAG"
     gene            42087..43340
                     /locus_tag="PK224_07935"
     CDS             42087..43340
                     /locus_tag="PK224_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB15705.1"
                     /translation="MYRKIVLDNRLRIVAELLPTLKSVTIGIWVNVGSRDEQPGEEGL
                     SHFLEHMFFKGTRSRTATQISREIDALGGEMNAFTTRETTTFYVKVLDQQLEAALELL
                     SDLFYRSRFESKEVEKEKQVVLEEIRMVQDDPEDLVQELHMKHTLGSHPLGRPILGQA
                     PRIQALGRNDLVSYVGSHYDPERTVVAVAGNFTWRRLEQLLARYFSDSHKGVAARPSR
                     RPPEVKGGVLVKRKALEQVHLCLGLQGLSAGHKDRYAAHALNGVLGGSVSSRLFQEVR
                     EKRGLVYSIYSFLSTYSDGGMTTVYAGTRPKEVERVVEVVCRELKKLRTHGIDAKDLA
                     RVKNQMKGSLMLSLESSHSRMSKLAKDELTQGNHVSLEQMIAEIDRVTTDQVYRVAQT
                     LLDQRCLSITALGPIPTKSLQSFAS"
     gene            43715..44227
                     /locus_tag="PK224_07940"
     CDS             43715..44227
                     /locus_tag="PK224_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15706.1"
                     /translation="MNRVGILAALAVSSVVGLLTAGVTLAADAPAGGGPSEVTTGSGK
                     FFKGETTNTLKGRVWSQWADNPDTELLFGIQYWNTGDPASGEGGGRSSTQMDVKPPDP
                     LFTCCGWGFTGGTDKNNPYAGWHHAQTTVRLSVKDKSLMDQIIKASQELVAMEVTLDG
                     RTITGFKVLK"
     gene            44288..44962
                     /locus_tag="PK224_07945"
     CDS             44288..44962
                     /locus_tag="PK224_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15707.1"
                     /translation="MKLQKQGWTFMAAVALVVGFASTALAIEAFQERFEWGDLSKPTT
                     LQGRVIILDPYDEAVWLNIAVFGGNAESGLFWQRIHPGKNLKFYPADKNVWEQLKSMA
                     RPHSGPAAAKEVPPGSPTTLVEFVAQEVEQNRRVISSVKKLADNAGEMGKPKSISSLR
                     LKECEGKTEVDAPCHAAKTLLNTSPKTPDGANLPLQYDVLLPNGKPLANLIPWTAKYN
                     QGGGHH"
     gene            complement(45068..47716)
                     /gene="mutS"
                     /locus_tag="PK224_07950"
     CDS             complement(45068..47716)
                     /gene="mutS"
                     /locus_tag="PK224_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRB15708.1"
                     /translation="MGDADSTPLMRQYRDIKQAYRDAILFFRVGDFYEMFQEDAVEAS
                     KLLSIALTSRDKSSETPIPLCGVPYHAATNYIAKLLRAGRTVALCEQVEDPKLAKGLV
                     RREVVRLYTPGTLIDSEFLPSTESNVLAAVYVRLRSAGTGRKEPSYGLATLEVSTGDY
                     WLSEFHGIQAEASLRDELARLEPKELLFPEDLTPDEQQWTADLTGPRCCAQPATWFDL
                     EKGRMTLQEQFRVHSMEAFGCHRLTLGIQAGAAVLRYVRETQPSAPLDHIRPPRVRQD
                     HDAMHLDSVTIRNLELVRPAGRAEQSSNQSTYTLLGVLDRTVTAMGSRLLREWLLRPL
                     VNSAAIEARLTAVDELKRQIDARVRLRTALRTVQDISRLCSRMSLGVANPRDVLALKI
                     SVSSLPAIQAELSGLDSPLIADLMSSWDNAQDLYELIEGAIEQEAPVSIRDGGILKSG
                     YHPEVDELRKASREGKGWITGLEAKERERTGVESLKIRYNQVFGYYIELTKANLGKVP
                     PDYIRKQTLVNAERFMTAELKELEERVTGADTKLTALEQALFEQLRTRLAEETARLQE
                     ISRRLATLDVVASLAETAALNRYVRPTVDEGDGLHIVQGRHPVVERLDLSGGFIPNDT
                     HLDLATSRLHILTGPNMAGKSTYLRQVALITLMAQMGSFVPASEARIGLTDRIFTRVG
                     ASDNLAGGQSTFMVEMTESAHILNCATPRSLILLDEIGRGTSTYDGLSIAWAIAEYIQ
                     DPQRLGARTLFATHYHEMTQLESLREGITNYCVAVQERDGKVLFLRKIIRGGADRSYG
                     IHVAELAGLPDPVIHRAKAVLAQLESSSSSGRSVQENQQSLQFDATLPAPHPMLEEVR
                     QMNLFSMTPLDALNRLAELQQRLLQE"
     gene            complement(47709..48053)
                     /locus_tag="PK224_07955"
     CDS             complement(47709..48053)
                     /locus_tag="PK224_07955"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRB15709.1"
                     /translation="MIRLILVGTLLLLALSFFLQNQEQEVTLRYFFGLRSASILIYKP
                     ILAAFGVGLLVSGILLFPAWVRGRIELRRKTKALQEAEVDLERLRQALDKAARRVGTS
                     SDIPAEGEPSDG"
     gene            complement(48050..48571)
                     /gene="tsaE"
                     /locus_tag="PK224_07960"
     CDS             complement(48050..48571)
                     /gene="tsaE"
                     /locus_tag="PK224_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRB15710.1"
                     /translation="MLRSPKHTHRLGRCLGTLLQGGEVLALFGELGAGKTSLVKGIAD
                     GLLAEPTDVSSPTFTLIHEYRGRLPLVHSDLYRLTAAQLEDTGLNDYLDGHTVTAIEW
                     ADRWGDGLPSDRLDVHLSHRPPAARRAILTARGPAARRLLDALRSRLSANRPTRAAQQ
                     TRVQLNRPRRASH"
     gene            complement(48648..50204)
                     /locus_tag="PK224_07965"
     CDS             complement(48648..50204)
                     /locus_tag="PK224_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRB15711.1"
                     /translation="MIPIVTAEQMRALDQRTITEALVPSLTLMERAGAGVVTHLEARY
                     APLAGKSVIILCGKGNNGGDGFVVGRLLRRKQAQVHILLLASPEDLSRDAKTMYQRFL
                     RSAGKSAVTCPADVETIQQRLATGDILVDAILGTGLSTPVTGLYRDAIEAITAVGRPT
                     VAVDLPSGLHADTGAVLGAAVRADLTVTLGLPKAGLYSGSGIDCAGTVRLVDIGIPAA
                     FVEAIGSRLMLLTDTAARAALPPRRPSAHKGTYGHLGVIAGSVGKTGAAAMAALSALR
                     IGTGLVTAAIPSSANDILEAKLLEVMTLPMPETKARTFARSGLDRLLAFAGARDAVAI
                     GPGLTTHPETVDLVQELVKRIDKPCVLDADALNALAGKSSLLTECKRPPIITPHPGEM
                     ARLETEATTQSVNEDRLGTATRFARERGVFVILKGARTVIARPDGLAAICPTGNPGMA
                     TAGTGDVLTGMVGGLLAQGLAPWDAACAATYFHGLAGDLAAQHLGQAGMIASDLIQHI
                     PHALAPTTHT"
     gene            complement(50273..50986)
                     /locus_tag="PK224_07970"
     CDS             complement(50273..50986)
                     /locus_tag="PK224_07970"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRB15712.1"
                     /translation="MARLGVNIDHVATLRQARGGADPDPLTAAILVELAGADGLVVHL
                     REDRRHIQDRDLTMLREIVRTKLDLEMAADDAMAKIALSVKPDLVTLVPERRQELTTE
                     GGLDVANHRDRIQKIVDLLRDGGIPTSLFVEPSLDQIKAAHKIGAAYVELHTGRYSNA
                     KRSKEEDEEFEAITQAAKLAYKLGLGVNAGHGLTYKNVKRLAKLPEIVEFNIGHSIIA
                     RSVMVGLVQAVREMRELVA"
     gene            51288..52484
                     /gene="alaC"
                     /locus_tag="PK224_07975"
     CDS             51288..52484
                     /gene="alaC"
                     /locus_tag="PK224_07975"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HRB15713.1"
                     /translation="MAIGDGFYRIKRLPPYVFAQVQTLKLEARQRGEDIIDFGMGNPD
                     QPTPPHIVDKLIEASRKAKNHRYSASRGITKLRHAITGWYKRNYDVDLDPETEAIVTI
                     GSKEGIAHLALATIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRQDSDFFDDLLR
                     VYRQTLPRPRILVINFPHNPTTAVVDLEFFKKIVAFAKEHDVIVIHDLAYADIAFDGY
                     KAPSFLQVPEAKDVGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKLKSYLDYGIFQ
                     PIQIASTVALNGPQDCVKEVVQRYQNRRNVLVNGLNRIGWPVALPRATMFVWARIPDP
                     FRHMGSLEFSKLLLREAKVAVSPGIGFGEGGDEFVRFALVENEHRTRQALRGIRKVLN
                     LDDQES"
     gene            52481..53794
                     /locus_tag="PK224_07980"
     CDS             52481..53794
                     /locus_tag="PK224_07980"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRB15714.1"
                     /translation="MKSEIGVGLIGFGTVGTGVARVLIENAELIRRRVGVPVTLVRIA
                     DLDITRDRGIAIPPGVLTTDIQQVLTDPRVDIVIELMGGYDLAKRVILDAVQRGKHVV
                     TANKALLAVHGEEIFAAASRQGIDLGFEASVGGGIPVIRALMEGLAANNIQSIYGIIN
                     GTSNYILSRMTSEGQRFDVVLEEAKRAGYAEADPTFDVAGIDSAHKLTIMVSLAYGTP
                     VNFKEIYTEGITGLTPLDIAYAKEFGFTIKLLAIAKYSDGEIEARVHPTMVPSASQIA
                     KVDGVYNAIQLVGDAVEDVVLYGRGAGSMPTGSAVVSDVMAIARNLLKDAAGRVPPAS
                     YQPDQRRPLRMRPMEEITSLYYIRFMVLDRPGVLSQIAGVLGRYGISISSVLQQGRKE
                     GQTVPVVIMTHMAKERDIQNALREINPMSYISEPTTLIRVEGRDE"
     gene            53840..54898
                     /gene="thrC"
                     /locus_tag="PK224_07985"
     CDS             53840..54898
                     /gene="thrC"
                     /locus_tag="PK224_07985"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRB15715.1"
                     /translation="MNRWRGIIEEYRKFLPVTAQTPVITLGEGNTPLIRAARLAKKIA
                     PGIDLYLKYEGMNPTGSFKDRGMTMAISKAVEGGARAVICASTGNTSASAAAYGARAG
                     IAVYVLIPAGKIALGKLSQAMMHQATVIQVEGNFDQALTIVKELSATYPVELVNSLNP
                     FRIEGQKTAAMEICDDLGDAPAMHVLPVGNAGNITAYWNGYREYRAANQTTRLPRMMG
                     FQAAGAAPIVLGHIVEEPQTVATAIRIGNPASWQSALTAVKESSGAIDMVTDEEILHA
                     YALVASEEGVFCEPASATSVAGVIKLSQAGQLKEGATVVCTLTGHGLKDADTAIGISR
                     QPQTVKATRDDVARLLHV"
     gene            54917..56167
                     /gene="apgM"
                     /locus_tag="PK224_07990"
     CDS             54917..56167
                     /gene="apgM"
                     /locus_tag="PK224_07990"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRB15716.1"
                     /translation="MKYLILHADGMADLACPELGGKTPLQAAATPNLDQIAQRGEVGL
                     LSLPSEAGAAGSDVTSVAVLGYDPKKYYPGPGPLEAAGLGVTVGEQDVVFRCTMVTVR
                     GESASGSKDRATDIKKLGPHVLMEDATAGLIDTEQARELLEAVNEQLGSESIQFYPGS
                     GHRHLMVWVGGKSRATCLDPQPLVGRSIADALPSGDGADVLRKIMDAAFFILRDHPVN
                     EEREQEGLKPANCLWLWGQGRAAVWPPLPERYQIAGVVVSSSDVHRGVGVCAGLDAVE
                     LPLADGSDANEFTGCVQAAVQELAKKDFVYLHAGLSDDVLHATDVQDKVRAIERFDAQ
                     VVGPILAALATHPAYRLLVVCDPGLAKSHGAEVPPILYALCDSAAKPSAGVTKRFHEQ
                     DANIAGTAPRDATKLMLRLFPRGV"
     gene            56172..57407
                     /locus_tag="PK224_07995"
     CDS             56172..57407
                     /locus_tag="PK224_07995"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRB15717.1"
                     /translation="MALIVQKYGGTSVGTVERIHRVAERVERAQKDGHQVVVVLSAMS
                     GETDRLLKLAHEVTGAPDERELDMLLSTGERVTIALLAMELRGRGVNARSFTGRQVGI
                     HTDSAHTKARISRVTADRIKEALSAGVIPVVAGFQGINASSDVTTLGRGGSDLTAVAL
                     AAALKADRCIIYTDVDGVYTADPNIVPAARRLDKISYEEMLEMASLGAKVLQSRSVEF
                     AAKYSVPVEVNSSFKEGKGTLVTREDADMEGVMVSGVTGDRNQAKITIVGVPDRPGIA
                     ARVFGAVANANIVVDMIIQNVSQASTTDISFTVPKADLRNAVDLVQRLSEEIGARSVA
                     VTESIAKVSLIGVGMRSHSGVAAKMFEVLSREGVNIMMISTSEIKISCVIEEKYLELA
                     MRTLHTAFGLDRVSAPALG"
     gene            57537..59126
                     /gene="cimA"
                     /locus_tag="PK224_08000"
     CDS             57537..59126
                     /gene="cimA"
                     /locus_tag="PK224_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRB15718.1"
                     /translation="MPSLTAAQASALEIYDTTLRDGAQAEDVSFSVEDKLRVAQRLDE
                     LGVHFIEGGWPGANPKDIEFFRTVKTIPFKNATIVAFGSTRKASNSVRKDKNLQELLA
                     SGTPTITLFGKSWSFQVTDALGIALAKNLELIEDSIHYLRSKDRRVFYDAEHFFDGYK
                     ANPDYALQTIRRAIAGGAERVILCDTNGGTMPWEIREICRIVKQECPVPLGIHAHNDS
                     EMAVANSLVAVESGILQVQGTINGIGERCGNANLCSIIPNLQLKMQRPALGNKLTHLK
                     DVSGFVTEIANLMPNKRQPYVGDAAFAHKGGVHIHAVLKNAATYEHIDPAAVGNRRRI
                     LVSDYAGRSGLLEKVEAYGISLNKNHAKLQELVETLKERESQGYQFEGAEGSFELLMR
                     KAMGSHRPSFQLLGFRVIVEKKQEHSLLLSEATVMVKVGEVVEHTAAVGAGPVNALDH
                     ALRKALEKFYPQLREVKLLDYKVRVLSADKGTESKVRVLIESGDHKDKWGTVGVSENI
                     MEASWQALADSIEYKLLLADR"
     gene            59272..59547
                     /locus_tag="PK224_08005"
     CDS             59272..59547
                     /locus_tag="PK224_08005"
                     /inference="COORDINATES: protein motif:HMM:NF001401.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRB15719.1"
                     /translation="MRKADIANEIFKQVGVSKNDAADIVELVLNLLKGVLQKGDAVKI
                     AGFGNFVVRSKGARKGRNPRTGEEIGITPRRVVTFRPSQVFKKYVNS"
     gene            59589..59942
                     /locus_tag="PK224_08010"
     CDS             59589..59942
                     /locus_tag="PK224_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRB15720.1"
                     /translation="MGNEPRLGSKVFYKIGEVSKISKLPAYVLRFWESEFSFLRPKKS
                     RGNQRLYVQKDVDTVLEIKRMLYDEGHTLAGVKRYWARRGRVTVKKLSSRKELAQRVR
                     GDLQAIIRMIDSHSS"
     gene            60002..60078
                     /locus_tag="PK224_08015"
     tRNA            60002..60078
                     /locus_tag="PK224_08015"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60036..60038,aa:Pro,seq:ggg)
     gene            60245..61069
                     /gene="surE"
                     /locus_tag="PK224_08020"
     CDS             60245..61069
                     /gene="surE"
                     /locus_tag="PK224_08020"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRB15721.1"
                     /translation="MARVRILVTNDDGITSPGIHAVAAALGALGEVWIVAPDRERTAV
                     GHAVTLHKPLRLTKMAPRVFMVNGTPVDCVNLALVKVLPGRPSLIVSGINRGVNLGDD
                     VMYSGTVSGALEGTILGIPSIAVSQEGNETFRFDVGAQYAARVAAEVLRHGLPPETIL
                     NVNIPNVPVRSIKGVKVTCLSRRRFNNPIVEKVDPRGRKYYWIAGTRQSWSRENDADH
                     EALEQRMVSVTPIHLDTTHHEVLEQFKAWEQPLSRPSARPKTVKPHTRRRAQAWPD"
     gene            61057..61692
                     /locus_tag="PK224_08025"
     CDS             61057..61692
                     /locus_tag="PK224_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRB15722.1"
                     /translation="MAGLIEWLIEVLGRFVIATISTFGYTGIVITMAIESACIPLPSE
                     IIMPFSGYLVSTGQFSMMGVTLAGAIGNVLGSLVAYYVGVWGGRPFVERYGRYVLVSQ
                     HDMELADRWFAKHGEAAVLIGRLLPVVRTFISLPAGIARMDVKKFVLYSFVGALPFCY
                     ALAYVGLKMGEHWNQLRQYFHHADLVIGLCLAVGLGYFLWSHWPKRRPSME"
     gene            61702..63162
                     /gene="cysS"
                     /locus_tag="PK224_08030"
     CDS             61702..63162
                     /gene="cysS"
                     /locus_tag="PK224_08030"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRB15723.1"
                     /translation="MLRVHNTLTGSKEPFEPLVPGKVRMYVCGVTVYDYCHIGHARSA
                     LVFDVLRRYLEYSGFVVEFAKNFTDVDDKIIKRANEQGVSCEHVTTTYINAYYEDMEK
                     LGVRRATLEPRATEHIADIVRLVDTLLAKGMAYRVDGDVYFQVDRYPVYGRLSKRNVD
                     DLQAGARVDVDERKRHPMDFALWKGSKPGEPSWDSPWGPGRPGWHIECSAMAMRHLGE
                     TFDIHGGGMDLIFPHHENEIAQSCGATGKEFARYWVHNGFVQINQEKMSKSLGNFFTI
                     REIFQKSEWSDTVTGEMLRYFLLSTHYRSPLDFSDQSLNEAKNALNGFYDLFERLNES
                     ASAHGAADQQMQESTMRARAAFVAAMDDDLNTPNALAALQTLRGEANKALEVGLSGEM
                     RRVVRQEFRALGIVLGLLQTDTWRFKSQRQQPSSGGAEASTDTLSDEDIADKLAARLA
                     AKRSKNYQLADQLRAELASHGITIEDRPDGTSRWKR"
     gene            63137..63913
                     /gene="rlmB"
                     /locus_tag="PK224_08035"
     CDS             63137..63913
                     /gene="rlmB"
                     /locus_tag="PK224_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRB15724.1"
                     /translation="MAPVDGNDNSLEVIYGLHAVREALRAGVRPIQRLLVLGTDRQFG
                     EIARLARDQRVPVRVEPRAALDRLVPSGHHQGVVGVVAAKAYAEPDDILASAQAEGQT
                     PLLVLLDGVEDPHNLGAILRTAEASGVQGVFIPERRAVGLTSVVAKASAGAVDYMPVG
                     RVPNLSRLIERLQGAGIWVYALDAEAPKPYTALDFSGPVALVFGGEGTGVRPGVRDAC
                     DEAAHIPMMGKVSSLNVSASAAIVLYEALRQRREGAGKKP"
     gene            complement(63931..64221)
                     /locus_tag="PK224_08040"
     CDS             complement(63931..64221)
                     /locus_tag="PK224_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB15725.1"
                     /translation="MYMSPVAKTTKSRAKVSAPDPVLPPRKRRPEALTTREQEILELI
                     WTGFKNKEIAQRLKISVKTVEAHRANMMKKIRVSNTAQLLKAAIQGKMLKLR"
     gene            64436..65209
                     /locus_tag="PK224_08045"
     CDS             64436..65209
                     /locus_tag="PK224_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRB15726.1"
                     /translation="MISYVTVLLFGAVIGSFLNVCIYRLPREESVAWPASHCPSCRQA
                     IAFYDNIPIVSYLLLRGRCRACHAPIAIQYPVVEAANAIGYVLVFWTFGFTPAAWAYA
                     ALASALIVVTGTDLSHTMIPDAITLPGIVIGLLCAILILPIGLVDSLLGVLLGGGILW
                     FLAWISPYVFGKEGMGGGDIKLMAMVGAFLGWQPVLLAIMIGSFLGSIVGVGLIAIGV
                     MRREQYIPFGPFLAVGSILALLFHQPLLEWYWALIDIPQ"
     gene            65501..65935
                     /locus_tag="PK224_08050"
     CDS             65501..65935
                     /locus_tag="PK224_08050"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HRB15727.1"
                     /translation="MNERGGSLVELCTVVAIIGIVVGVSVPGWAALVAKHQQRAVMVE
                     IASELRMARQLAMARHERIRVVVDAEQSELRTECMDCDTSLLRRYEFAHKGTAVESMT
                     TKPEIMFQPSGRSATATTMVLVDGRRATHQVTVSITGRVTLS"
     gene            65932..66459
                     /locus_tag="PK224_08055"
     CDS             65932..66459
                     /locus_tag="PK224_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB15728.1"
                     /translation="MTQCEPNLVTRRSALMSAKGFTLVESMVALVVLSIGVIGTIGMC
                     EWAERGLQRGALTTTALALAESRLEAKRSVAWERLLMDDVDQDGIVESVMHDDGLQDD
                     QTNGDGIFTASATQSNIRLVWTVELNRGHEPAGASLATIEARAIFRTMGGQEKEVRVR
                     TIRANPRYVGLSVLS"
     gene            66468..67232
                     /locus_tag="PK224_08060"
     CDS             66468..67232
                     /locus_tag="PK224_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15729.1"
                     /translation="MVNGSNAPKRMNIGWCLGSAGASLIELLIAMAISSIAISASIHV
                     FSSVGLRFSGQHSTMVTNQDLRLGLDVLCSEVRLAGAGLLGEDSPFLKAKSDEIEFFA
                     NLSGASTTLTQVAEIGRQDLPVEDGAGWPKGKQLLVCTAVHCAWSQLAADGRKQTLTL
                     VTPTAEQFPVHSAIFLLNRVRYYVKRQDDGAMRVMRDVDGGASTLLGDVSQFELQYFD
                     REGRVTTDLSEVVRVRVAIQAGRQGARLVRDVAIRM"
     gene            67263..68738
                     /locus_tag="PK224_08065"
     CDS             67263..68738
                     /locus_tag="PK224_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15730.1"
                     /translation="MGRSRIAVRRDERGMALLAVLMVVFLLTLLGMTSMQLAGQEIVG
                     ASALQEERLAHHAAEAAVDVVMGWFHDPALHPQGVETTWLTKRLLNAQGDASYFDAQG
                     RAQFAGTGASPDVVFDAAHAQHDRLMNDPQTGWFKSLKGLARILKLRVYGATRPGLLC
                     TVEVTAGAGHTSRVTKTVSVEFGAYAVPALHAPIQGGTVGNESAPSSAGSVFAHWGDM
                     VVRGKAYFPRPEAVPMKSGLAPVTGQSYDEMTHREDRWFTIRLGGDAFFAQLPAEAWS
                     GVPLNLHTHQDPVPGLKVDQWDYDTLKRMARQFGQYYGIDRDGLLYAGGVIQPGAGHP
                     ASEVFASKGPGDHRGLIFVDTLDGMPPRPDNLGTIVLDQEYAEGIFIVNAHVLWKAGA
                     HGKPVSALSPPPEGQQSLGARIPVQLSGIHLQGVLYVAGDVRYAGHLKVYGGVVAQGA
                     IVDGTNGSGMLEAWCNHDLRDGLVQGMPLVFVAPGSWQAKI"
BASE COUNT        13641 a        18228 c        22195 g        14753 t
ORIGIN      
        1 cggtcgagct gttgcgattt atcgaggtgc tggaacagac gctgggcatg aaggcccaaa
       61 agaacttcct gcctctccag gcaggcgatg tgccagcgac ctatgccgac gtcgcggatc
      121 tcatgcgtga tacgggcttc aagcccgcga cctctatcga gaccggcatc gcccgctttg
      181 tggaatggta tcgggcgtat tacaaggtgt gatggcatgt ggagcgcgtg ttcctgcagc
      241 gtctctcccg gggggtggct gagaagctcg tcaagccact cggtcacctc cgcacccacc
      301 tggtcgcgta gtgcgataaa aatcagcaga gttgctcgaa cccgtgcaag cgttcggtga
      361 tgtccccgca gaggggatgt gcgacgacgg gtgcaatggc cctgaattgc ttgatcgtgt
      421 gaacctgatg ttccagggtt ccccgccagt accggatcgt ccttcgtcct tgtagttctg
      481 cgacgcggtt ccattctgct gtcggtaagc gcagtcaaac cgtcccggca aattaagatg
      541 agtcgcgtgg cgcggcgtct cgagtcgttg cgagccgact tgtctgggat gctgagtcgg
      601 tacttgttgc agctgggagt atggagtaaa ttagaaatag agcctctccc tttatgcaaa
      661 gggcttcagt ggacgtgaaa agaaatcaaa tgatttggtc gttgctcgca gcaggatggc
      721 tgatgctggc ggggcagggc cttgcgctgg cagaagcgcc ttcgaccgag acagcgacgt
      781 ggagtgagtt gcgtagccaa tcatcgaacg ccttgtctca ggctccggca acgagtgaga
      841 ggcaagtcga catctttcgg cagacgccca tgttgagtgg tcgcttgcag gtcagtgagc
      901 agacgctgat cccttatgtg ggggccggat tcagcggtgg atacgtgacg gagcgggatc
      961 gggccctcgg tcccatgccg ggactcccgc agcaaaacct cttgggcgac tcgttaggtt
     1021 tgggcaaggg catgatgccg aacgaatttc agatgggcat tcgcattccg ttttaagtcc
     1081 tgccggttgc gtgcggcatc acagcatgaa tcatcccttc tggtccagtg gctgaaagag
     1141 tcgccgggcg aggcctgccg ggtcgattcc gttcaagctc gggaggacca ggtgcgcctc
     1201 cgctagttgg gatgccgggt aggtcgtcgc aatggcgagg acgcgcatgc ccgccttcag
     1261 gcctgccaga actcccgccc gagaatcttc gatcaccagg cactcgtttg ccctgagcaa
     1321 cggtggacga ggcctacggg cgttcagcag tctgagggtg tactcataga tcgcagggtc
     1381 cggtttcccg accgcgcagt catctgcgga cacgatcaac tcaaatgcct gttcgatggg
     1441 ggtgccggtg agtgcggcga ggatctgttc gcgtctgcct ccggacgcca ctgccagccg
     1501 gtacgctccc gacgcgcgct gcacgaactc ggtgacccag ggaaacaatg cgggtttgtg
     1561 cagagccgta tgtgcgcgaa acaagccggc ctttcgttcg ataatcccgg catgaatcgt
     1621 cgggtcgcag atcccgtcgc gtcgttccaa taatgcggtc gcgcaggttc gttcatccat
     1681 acccaggtag gtgccgtagt attcctctct gctcaggctg agtcccgctt cggccaacgc
     1741 ctgctgaaag caaagcaggt gtggtgtttc atcgtcggcg atgacgccgt tgaagtcaaa
     1801 aatgatggcg cgcagagcgg cccccctcgg ttcacgtcat cctaccgtgt tgtgcgctac
     1861 tctagccgac cgttcgctgt cggacaagcg gaactccttg tcccgctgag gcccctacgg
     1921 tatgctgcgt cacggcaacg tatccggacc gtttgccacc acaaatcgac tcccatgaca
     1981 cgatgagcga ctacgcagtt ctcggcgatc tcttggtgat ctatgcagtg tccactgcgg
     2041 tggtctttgt atttcatcaa tttcgcctgc cgtcgattgc cgggttcctg gtggccggag
     2101 ccctgatcgg gccgcacggg ctccatctga ttcccgatgt gtcgcaggtg caggtcctgg
     2161 cggagatcgg aatcgtgttg ctgctcttta cgatcgggat ggaattttcc tccgcgcact
     2221 ttgcggcagc ccggcgcatt ctcatggttg ccgcgccggt tcaaacgggg ggcgtcctcg
     2281 ttctggcgct actcggagcc ctggcggtgg gtctctcgta tcaacagggg attttctggg
     2341 gctttctcct ctcattaagc agcacggcca tcgtgctgaa agccctttct gaacagggtg
     2401 agagcgattc gtttcacggt cgcgccactg ttgcgattct gatcttccag gaccttgcgg
     2461 tcgttccgat gatgctcgtc acgccgatcc tgggcactcc gagcgggagt gcgatgggga
     2521 cggtactgat gactttggtc aaggcggcga ttgtggtcgg gttgatcgtt gcggccgcct
     2581 ggtatctcgt gccgcggctc ctgcgccata tcgtgcggag tcgaagccgt gagctgtttc
     2641 tgctttcgat catcgtgctt tgcctcggta ttgcctggct gacctcgctg ggcggactgt
     2701 ccctcgccct gggcgcgttt attgccggcc tggtcatttc tgagtcggaa tatagccatc
     2761 aggcattggc cgaagtcctg ccgttccgtg acagtttcaa tagcctgttt ttcgtgtcga
     2821 tcggcgtctt gatggacata cgggtggtgc tggaccatcc gttcattgtg ctgggcctgt
     2881 tattggccgt gattgtcgga aaactggtca ccgggagtgg ggcgatggtg gcggctggcg
     2941 ctccgcccag gtcgtcggtg ctggtcgggg tggcgctcgc gcaggtcggc gaattcagtt
     3001 ttatcctggc gcaacagggg caggatgccg gtttgttcac cggggatcag tatcagttat
     3061 ttctggcggt gtcggtgctg accatggtgg tgacgccgtt tttgatgcaa tggtcgcccg
     3121 atctggggcg gcggatcgaa gcgatgcagc gcgtccgagg ctggatgccg acccgcaccg
     3181 ccgcgcatgt cgagcagttg gaaagttctc agatccggat caaagaccac gttattattg
     3241 tgggctatgg gctcaacggt cgaaacctgg cccgcgtgtt gagcgaaacc gaaatcccgc
     3301 atgtggccct cgacctggac ggcgaaacgg tgcgccgcga ggcgcgtcat ggcgtgccga
     3361 tctactacgg cgacggctcg aatgccaatg tgcttcggca tatgaagatc gacgacgcca
     3421 aagtgctggt cgtggccttg tccgatccgt tcacggccag aagaacggtg aaggtggcca
     3481 aaggattgaa cccgaagctc catatcgtcg tacggacgcg ctatttgaga gagctggagg
     3541 agctgcatca gctgggtgcg gacgatgtgg tgccggaaga attcgaaacg tcgattgaaa
     3601 tctttgccct ggtgctgcgc acctacagtt tgccgcagga atttgtggcc cgaaaagcgg
     3661 agcaaatcag gcgcgaaggg tatgcactgc tgcggcggag cgagatgccg gagttggctc
     3721 atcatctccg cggggggacg ttgaccgatg tcgaagtgga aacctgccgg atcgatgacg
     3781 aggctccggc ggtaggcaag tcgctggcgg aactctccat tcgcccacga acgggggcgt
     3841 cggtgattgc ctggactcgc agccaggtca ctcagtcgaa tccctccgaa accgtgcggc
     3901 tgcaggccgg cgatgtcgtc acgctgctcg gatcccgcga ccagattcgt cgggccatgg
     3961 cgctcttgaa cgagcctaac cacggcacga gtcaccagct caaataaccc ggtcggctaa
     4021 cttctccagc gaagcgtcac acggtctttc agcggatggt ctaagggacg tccggtttgg
     4081 acggcggcga gatgggcggc cttcagtttg tagtaccact tggcgggcgt atcggcgtca
     4141 tcgatcagca tgagcgccgg tttgtatttc agtcccacgg cttgccgctc catcgcctgc
     4201 ttgtcctgct tcatgaagat attggcgaaa taccggaaca gcggcttggc aaagggcagc
     4261 caaggaagga tgttccaggc cgcagcaaaa tcgatccggc attgttgctc acccacgggc
     4321 gtgactgtgg cgcgcgacga gacgtacatc gaaccgcatt gtacgaactc gaagcgttga
     4381 ttggggagta cgaagtcgat agtcgtcgtc aacggcccgc cgtagagccg gttgaggagc
     4441 ttgtagggtc cgctgttctt cgacggcgca tggggaacca tgcgaaaccc gttcgggatc
     4501 ggctcgaagg tcttggcctt gtcgtgcatg ctggctttgg tccgccacca ggaactctgg
     4561 tgcacaaagg gaccgtgggc cgggtccatg agtccgacga tgccgtcgtc gatggtgcag
     4621 tccaggatcg ttgagatttg gatcatgcga tagggttcgg acgggagcgg gagacgcggg
     4681 agttccggaa ccggttgttt cggctgttgc ggatccggga gaaacaccca cacgtaccca
     4741 tcctgatcct ggcagggata ggacgtgatg ccgatctttt cgggttggag cggggaccct
     4801 tccacgagtg ccggaatatg gcggcagcgg ccaccggtat cgaactgcca tccgtgatag
     4861 gcgcattcga cgcgctctcc atcaaagtgt ccgaacgaga gcggcatgcc gcgatggggg
     4921 catatgtcgc gcatcgccgc caccttgcct tggcggtcac ggcagacgag gatcggttgt
     4981 cctaacatca cctgggtttg catctggccg acagccagag ccgtgctcgt ggtcgccgga
     5041 taccagaagc cgtagagcgg ggcgctcttt gtcggtttga gttcggtgat gaggtctgtg
     5101 ctcatgagtg atgcagtcgc cgggtttgtc gcgatgcgtg agacggttcg ccgccgaatg
     5161 gactataacg gcgggcccgc aacaggtcaa gcaaccggca gtctagggcg aaggccgcag
     5221 acggtacgat ctggatggcc aaaccccgct tctgacccgc atttcttgac aagaaaaaga
     5281 agcggagact atagtcaatt tccatcgtat gtgactccat atccctactc cgagagacga
     5341 ggtaccctca tgacaaccgc acaaatcgag cccaccgctg cactggcgga actccgcgag
     5401 gcgaaaccgg atcgtgtgac gattgtgttg ctgagcggag atctcgataa ggccatggcc
     5461 gccttcatta tcgccaccgg tgcggccgcc atggggatgc aggtgacggt cttctttaca
     5521 ttctgggggc tcaacacgat tcgcaagaaa ggggcaagca gttcggcccc ggattggttg
     5581 cgccggatgt ttggtttact gaacaagggc ggcgccgaga cgttgccgct gtcccgcttc
     5641 catttctggg ggctgggcac gaagatgatg caagtggtga tgaagcagaa tcgtatgccg
     5701 ggtgtgccgg aactcatgga gacggcgctg gagctcggtg ttcgcttcat cgcctgcacg
     5761 acgacaatgg gcctgatggg catcacgaaa gatacgttga tcgacggaat cgaccaattt
     5821 gccggtgtga cgacctacct ggccgaagcc aagcagggca gcgtcaatct gtttatctaa
     5881 gcgaggggtt gaggagcggc acgatgcagg ctgatgtgaa actcgatacg ctcgggtatt
     5941 tttgcccgat gccgattatt ctgacgtcca agaaaatcaa ggaactgacg accggtcagg
     6001 tgctggaagt cgtgtcggac gatgaaggca tcaagaagga tatgccggcc tggtgtgaga
     6061 cgaccggcca cgaaatggtg gggctcgaag aggagcagag tgcgtccaag cggatctata
     6121 aggccttcgt caagaaaacc aagtaggggc agcgacaggc gcgcgaacgc ggggccgtcc
     6181 tgttaggcaa agccggaggg catgatcacc agcctccggc ttttttgttg cctcagactt
     6241 ccagggtaag tggggcggac cgggtcgggt gagagaggga acctcgtgag tcaggttgtt
     6301 gaatgtgtcc cgaacttcag cgagggacgg aactccgaga tcgtcgacgc gttagccgga
     6361 gtggtccgct cagtacccgg cgttgtcctg ctggatgaaa cgaaggatcc ggaccatcac
     6421 cgggccgtcg taacctttgc cggtaggccc tatgccgtgg ccgaggtggc ctatcagatg
     6481 gcgcggatgg cctcccagtt gatcgatctc cgaaaccatc atggcgaaca tccgcgcgtc
     6541 ggggcgacgg atgtgatgcc gtttgtgccg attcgtggcg tcagcatgca ggattgtgtg
     6601 caactggctc gcatggtcgg ccagcgtatc gggaatgaat tgaagatccc ggtctttctg
     6661 tacgaacagg cggcgaccag gcccgagcgg aaacagctgg agtggattcg gaagggtggc
     6721 ttgaagggac tggcggatcg gatggcaagc gaccctgcgt gggtgccgga tttcggcccg
     6781 aagctcctgc accagacggc cggggccacg gtggtggggg cgcgatggcc gctgattgcc
     6841 ttcaacgtga atttgaagag ccgcgatctt tcggttgcca gggccatcgc caaattggtg
     6901 cgccaatcaa gcggcgggct cccgtacgtc aaagcgatcg gcgtggagct gaaaagccaa
     6961 caactcgtgc aggtatcgat gaatttgacg aaccatgaag agacgccgct ccatgtcgtg
     7021 tttgcggcgg tgcagcggga agcggcggct cgtggtgtgg aggtggccgg cacagagatc
     7081 gtcgggttgg ttcccgaaca ggcgctcatg gagacggcgc agcaggcgct ctgtctcaac
     7141 caattcgacg gacgacaggt gcttgagtcg cgattggacc gtcccgaatc gcgggaggcg
     7201 atcgggcgga tggcggcgtc gcagccggtg aaggagcagt cgccgttgcc cgcgaaggtc
     7261 ggggcgccgg acgggaagat gccggagctt caggggcaga ccggcggcag cgtgggggcc
     7321 cagtcggcgg cgtttgcggc cgggttggga atgatggtgg cgaagctgac tcgcgcgcgt
     7381 gcgattgaaa cgcgcttatc cgaaattcgc acacgcctcc acgaattggg ccaagcggat
     7441 cgagatgcct atgggcgggt gctccaggcg agaaagcttc cgatcaccca tcctgaccgt
     7501 ccgctccaac tctcatcgaa cttgctgggg gcgatagaaa cgccgttgga aattgtgaag
     7561 ctgtcttgcg aactcattcc cttgctgcgg ggcctcctga cgcaaacgaa accggagctt
     7621 cagccggatc tgaagatggg ccttcgtctt gccgatgccg tcatcgatgg gtgcctcgga
     7681 atggtcgagg agaacatgaa agttcaacca aatcagctgc ttattgcctc gattcgagag
     7741 cgattctccg ttgcggaaca aatgcttgtg gatgcgaaat cgctatgcta cactccgccc
     7801 ttcgattcgt gggcccaaaa catgttgaat atcctgaaac ttcggtgaaa gagagtcgaa
     7861 ggtagctgac gatggaaatt aaggttttca ataacaacgt cgaaaaagcc ctgaaggtcg
     7921 ccaagaagaa attggcgggc gaaggattgt tccgcgaact gaagcgccgc cgctattatg
     7981 aaaagccgag cgttcggaag aaggcgaaag agcgagaagc gcagcgccgc cggcagaagt
     8041 ggctcgcaaa gagacggccc gaatagacgt caggcgttcg tttccacagc cgactagttc
     8101 gaccactccc tgttgagttc tcctctccgg cagctccatg caggcgcaag agatccacgc
     8161 cgccatccga acggtcaagc gggaaatcgc tcgctggcca gacccggttg tcggcgtggt
     8221 tgccagacag tcaggccgtg atcccttcct cgtgctcatt tcctgtctcc tcagtctacg
     8281 caccaaagac aagaccacgg ccgaagccag cgagcggctc ttcgccttgg cgtccacacc
     8341 ggccaccatg cagacgctga ccaccccggt cattgagcgc gccatctatc ccgtcgggtt
     8401 ctatcggaca aaggccaaac agattcagca gatctgcgcc caactccgtg agcggtacca
     8461 gggtcgggtg ccggacaaga tcgatgaatt gctgacgttg ccgggagtcg ggaggaagac
     8521 ggccaatctg gtggtgacgg tcgggtatga aaagccgggg atttgcgtcg atattcacgt
     8581 ccaccggatc agcaaccgat gggggtacgt caagaccaag agtcccgatg agacagaaac
     8641 agctctccgc gagaaacttc cgcgcaaata ttggatcaca ttcaacgatc tgctcgtgcc
     8701 ctacggacaa cacctctgcc agccggtctc gccattgtgc agccagtgca agattgcggc
     8761 gtattgcgat cgcgtcggcg tgacgaaaag ccgttaagcg tggagcgccg atacgcttca
     8821 agccaccagc cctttagtga gatatgaacg acgagagatg tcagatgaac aatttggttt
     8881 ccgcctgcgc agtgagcgag aggatggagg ggagcccaag cacttcgtcc acgttgcgtt
     8941 ccaccgcgcc cgcttccact cccatatatt cgagacccgc gctgcaggca atgagacgga
     9001 agtgcccgac cagacgtgct tcctggaaca tctcggagat cttcgggacc ttcttttcct
     9061 tcagaagccg ggcgacctgt tctgccgagg ccgcatattc gggtggaaag tcgatctcgt
     9121 cgatctttcc ttctgcgagt ttcttaattg tccagaacag caacaccaca atgacgtcct
     9181 tgcccatcgc ggcggcggtg aggccgaggg tcgcgacctg gtgcagtttg tcgtaggtgg
     9241 cgttgtgggc aaagatgacg aatttgggtt tacccgacat ggcagctact tgaccttctg
     9301 tctggttttg acgctattca caaagtcggc gatggctaca tctacttcag cgggtgtcat
     9361 ggcgcgatcg tgcatctcgc tctcgtcgag gaggtagcgc tccccttctt tttcctgtcg
     9421 caccaccacg gagttttccg gtgtgacctc gacccgcatg aaccattcct tcttaccttg
     9481 tgtaatgacc acttctttac caagcccgat cttggtgatg tcgatctgaa gttccgactc
     9541 ggtggtgcca tcagctgccc aagctgcgtg gctgctgagc gcaacggggc acaacgagag
     9601 ggcgaatccg agaacggcca ggccggaggc ctgaaggcag gctctcgtca ctcgtgcgct
     9661 gttgagtcgc atcggcggca tggtgcggaa ttatagctgg cggagcaggc aacgtaaacc
     9721 ggcttggctt cgccaccctt tccgccatgc aaagccccat gctaggatgc gccggtgata
     9781 agcgaactcc tgacgcagat cgaatcctac gtgccggcga atgtgctgat ctggttcgcc
     9841 gtttcctccg tgttcatgtt cgtggggacc ttgatcgcga ttcccatcat tttgatgcgg
     9901 ttgccggcgg actatttcga tgtccgcact ccgcgaccct ggatggagaa ccatcatccg
     9961 atccttcgcg tgattggtca tgtggtgaag aacgtcgtgg gcgcgatttt tctgttcgcc
    10021 ggttttctca tgctgtttct gcccggacag ggcgtgctga cgatgttgat cggcctctcc
    10081 ttgattgaat tcccaggcaa gcggcgggtg gaggccaaga tcgtggggca gtccaccgtt
    10141 ctcagcacca tcaacgccat gcgggccaag ttcgacaaac cgccgctcat cattgcaccg
    10201 gattgagagc gctgcggtaa gttcgactcc tgcgtgttgc tccaccggtt acattatctc
    10261 agctatgccc acactctatc tcatcgacgg cagcgcctac atctatcggg cattcttcgc
    10321 cctgcctccc ctggccaatt ccaaaggcct gcagaccaat gcggtgtatg gcttcacgac
    10381 gatgttgctg aaggtcctgc gggaccatcg gccggactat gtggccgtgg tgttcgatga
    10441 aaagggcccg acccaccgcc atgaagcgtt caaagactat aaggcgcaac ggccacctat
    10501 gccgcaaggc atgagcgcgc agattcccta catccatcgt gtggtggaag cgttatctct
    10561 gccggttatt cggcaggccg gatacgaggc cgacgatctg atcggcaccc tggcgagaaa
    10621 aggcgaagtc gaaggcctcg acgtcgtgat cgtcaccagc gacaaagaca tgttccaact
    10681 gctcaccccc aagacgcgca tttacgatcc cgtgaaagat aaatggttcg gtgaagcgga
    10741 ttcgcaggca cggttcggcg tggagccggc gcgtgtggtc gaaattatgg gcctcatggg
    10801 tgacaccagc gacaacattc ccggcgtcaa agggatcggc gaaaagacgg cggtgaaact
    10861 gattacgcag ttcggcacca tcgaagagct gctgaatcga ctcgaagaag tcacgccgac
    10921 aaaaactaag aatttgttgc gggagcaggg cgagcaggcc cggatgagca agcagctcgc
    10981 gacgattcag ctggactgcc cgctggaatt tgtcccggcc cattttcagg cgaaggcgcc
    11041 gcagaccgac acgctggtga gcctgttgcg cgagctggag ttcatgacgc tggccaaggc
    11101 ttttcaggga gagacgcctg agcacaatcg attggggact gatgtcgaac agattcatga
    11161 tgctgccgcg gcagacgcgt ttctgaagga gcaaccggca gttgcgatgt tgggcatagc
    11221 ctgcgtcctg accggtgagc cgggggtgcg cgccgatatt caaggctgcg cgttgggtcg
    11281 gtccgatgga cacgcggcct tcgtgcaggg cgaggcgcgt gactggccac gcccactcat
    11341 tgaatgcctg catgatggga gtaagccgaa agccgttcag gatctcaagc cgctgttgct
    11401 ggcgctccac cgtcagggca tcgacatgcc cgggccctat ttcgacacga tggtggcgga
    11461 ctatctgctg aatccgaatc gccgcgccca taccttggaa gcgattgcca tggacctgct
    11521 gagttatcag ctgggtgcgg gggtgagcga agatacaggc aaggggccgc aatccctgtt
    11581 cgatgtggat gagaatctgg ttcgtcgaac cggcgaggcg gcagccgtga cagccaaggt
    11641 ggccccgatg ctctgcgagc ggctgcgaga gcagggcagc ctcccgctct ttcaagacgt
    11701 ggagatgccg ctcgtgcctg tcttagccga gatcgaacgg aacggctttc tgctcgatgt
    11761 cgagggcctg cagactttga gcaaagaact agagcgtgag ctggagcaga tggtaggcag
    11821 catttaccgt ctggccggcg gtgagttcaa catcggctca ccgaagcagc tggcgaccgt
    11881 gctcttcgaa actctcggcc tgaagccgct gcgaaaaaca aaaaccggct actccacgga
    11941 tgaagacacc ctcacgcaac tcgcctcgca gcatgacctg ccggcgcaaa ttttgaacta
    12001 tcgaacgctg acgaaactca aatcgaccta tgtggatgcc ttgccgcaac tgatcaaccc
    12061 cgagaccaag cggctccaca cctcgctgaa ccaaacagtg gcggccacgg ggcggctatc
    12121 ctcgaccgat ccgaatctac agaacattcc ggtgaagggt gactacggtt tgcgtatccg
    12181 cgaagccttt atcgcccccc cgggtcatca actgctctgc gccgattaca gtcaggtcga
    12241 gccccgtatt ctggcgcacc tctcgcaaga ccctcgcctg ctgcaggtgt tcgagaaggg
    12301 cgaagatatt cacatggcca cggccatgga gatttttaac ctgcctgccg gagaggtcac
    12361 gcgtgacatg cggcgcgcgg cgaagagcgt ggtattcgga attgtgtatg gcatcagccc
    12421 gttcggcctt gcatccaaca tcggtgtgcc gcaagcggat gccaaaaaat acatcgagac
    12481 gttcttcgag aaatttgcgg ccgtgcgggc gctgatggat cgcaacatcg acgacggcaa
    12541 gaccaaaggc tatacgacca cgatcctcgg ccgccgccgt ccgattccgg aattgcaggg
    12601 cggcgatccc tcgcagcgcg gcgtcggcga gcgcatggcg gtcaatagtc cgattcaagg
    12661 atcggccgcc gatctgatca aggtcgcgat gatcaaggtc catcaacgcc tccagaacga
    12721 actgcctcgc tgcaagatga tcctccaggt ccatgacgag ttgatcttcg aagtgccgga
    12781 ccaggaattg gaaccggcga aacagcttgt gaaagccgaa atggaagcca ccggcgcggc
    12841 gttgggtctc tcggttccgc tgaaggtcga tctgggagtg gggttaaact ggcgagtcgc
    12901 ccacccttag ccggatgttg aaacaggctg ccagcagcgt tctcagtcgc acgcctccct
    12961 gcggccttgc ggacagcctg tttgaacatc ctcaacggaa gctgatgttg cacagcgcgt
    13021 gagtcgatcc agttcagcct atgttgccgc gaaagcgtac attgaccata cccgaaccgg
    13081 agtttcaggc cttagtgcag gaggcgctcg acggccttcc cgacgaatac gccaaactca
    13141 tcactaacgt tgctgtggtg gtggaggaag agccccctcc ggaagtgctc gccgacctgg
    13201 agatggagga agacgaagat ctcctcggcc tctatcaagg tctctctctc gacaaagaat
    13261 cattcttcca ggcaggcgga caactgccgg ccagaatttc catctaccgc ggaccgatcc
    13321 tccgcctctg tcgaaccaag aaagaagtcg tgcaggaagt gcgcgatacc gtcgtgcacg
    13381 agatcgggca tcatttcggg tttgatgacg atgaaatgcc ttactgagaa tagggccatc
    13441 agttacgttt ttctagggaa ccgtatctct cctggctctg cgctaaagat gtcatagcgc
    13501 agagccgtcg tgcgattgtt cctctcattc acgggtgact gatgaagaaa ctccatgtct
    13561 cccaatcgct ggtcgaggtc gaatcactga aagatttgct tgaaggtgcg ggcattgcct
    13621 gtatggtgag gaatcaacag agttcctcac tggccggatc ggttccgttt gtcgaagtgt
    13681 tcccagaact ttgggtgctc aacgatcagg attatgacat cggaaaagag ttgcttgctg
    13741 ggcaggcttc aacggccatg ccggggtctg attggacatg tgcagtatgc ggcgagtgtc
    13801 atgcgacgga gtttgtcact tgctggaagt gtggtgtgga gaagggggtg gaaggatctc
    13861 agaaggttgc gattcagtca gaggcgtcgc cttcaggagc ctcgctcgga tttggtgttt
    13921 tgctcggggt ctgtctcgga gttgccgcca cactgtgcat gtattggctg tggagatatt
    13981 cttcaatgac aagcacagcc tatgatcgga acgcggacgg caaggacgat gttgtctatg
    14041 agtataaggg gcaattccca tgggctgcca agttggataa tgattttgat gggtatttcg
    14101 agactgcgct tttctacaac caagctggtg aggttattcg tacggaaatc gatcgcggtc
    14161 gggccggacg gccggatctg attgaataca atgcactggg aagacttgac tcagttgaat
    14221 ttattgaccg aaccacaggc agagtcaaaa agcgcatgta ttacaagtta gatgctaaag
    14281 tgcgtgagga gatcgatgaa gatggtgacg gcaagtttga gcgggttatt gagtttgatg
    14341 agttcgaaaa tcccaagtga taagcaatcg ccgttactcc gtctccggca gttcttcttt
    14401 gtaatccacc agcaggcact ggtcttcgct cgggaaacgt ccgggatcga ataattccac
    14461 catacttctt aaaatcctgc cccacctaac gctcagcaag tccgagcatc gttttccagc
    14521 ggtcaacgta cgtccaatcg acgcgtaagt ccgtctggcg cgtgcggatt ttctccaaat
    14581 cggcacgatc ctggagtcgt cccgccacca tcttgaggat gacgagatcc tcgatcgaca
    14641 gcatcggaac ctgtagcgcg ccgaagggaa ccgtgtgtct gcgctccaat gcggttcgaa
    14701 gataggccga gtctgccaaa aggaagtcga tcacgacttc ctgtccggag cggattccca
    14761 caattcgcca aatcgagatc ccctgtaagg ccatagggtt cggatggatg atggtggagt
    14821 cgaagaggga tgagacgagc gcctgtatct gttcgcgtga aggcggatcc agcagaatga
    14881 ggaggtcgat gtccgtggtg gctcgaggct ctaccaaggc gctgtagacc cagcctccgg
    14941 caagcgtaag caggatgcgg aaaaagtccg ccagcggcgt tctcgcgtca ctcagcggct
    15001 caccgtacca caagagtacg attcgcccct tcgttcgctg cggccttgct ggacggcctt
    15061 tttgcgcatc ctgcagcgtg tagtgacggg ggacggcacg tggagcccag cgtgcgccat
    15121 gataaaaaaa gtgaattttc ccacaacctg ttaggccacg ccgcgtcggt tgaagtcggt
    15181 gatgaggtcg gagaacgttc ggactaggat tggctctgct gccatgcctg ctctttggcc
    15241 agtcgaattt cgtgcatgac ctgcgcgatt ccctcacccg cgcccggcac attccgctgc
    15301 tcgatcacgg cttgacgcaa ggccagcccc aactcggtca gttcgatcgt gcggcgcaaa
    15361 ttgtcggcaa tacgtaagtc gtctggggat ggggatgatg gcgacataac cggggctccg
    15421 agcaaagacg gattctgaag gagagtctac ttgacgactc ggtcgggagc aagcgggaag
    15481 acgcccgctg cctgggttct ccctcccgta gaaggagtgc atcggacatt gatgggagat
    15541 cagcaaaccc ggacggggtc agagctcagg cttatggata cagccggccg acgatctgat
    15601 tgaagttaaa cagcatcgcg ttggtgcggg tgtaggcggc gtggccgtag tagtggtcgc
    15661 ggacattggt tgagctggtg cagtctttgt agtccacgag caggcactga ccttcgctcg
    15721 ggaaccgtcc ggcgcgtctg gcgcattcca cccagcgttc gaacccatcg tagggttcca
    15781 gcaattccca cacagcctta ttggcggctt tggcattctc agcgggcagg gcatcgctgc
    15841 ctgccacggc cagttcctgc acatagtcgc cgccccaggc gatcggcatt tcaacggaga
    15901 tctgcggcag ttccattttg gagagccagg tcttcccgtc ggtgcgtaga ttgcgatggt
    15961 tcacgacatg gagcagcttg ccgatgccgg acggatccca gccgtaccgc actggtgtgc
    16021 cgaaggtgac gatatcgagg gccccgccat tgagcaaggt gccattcgtc aataacgggt
    16081 cgatgcgctg aatggcctgc gcatcctgac cttgtggatt cgcggcgagg agaagatcga
    16141 agagggtctt ccgtcccgtg atcggcgaag ggcagaggag attagaggcc agcgcaagga
    16201 cgagcccggc ttggccatgg gcttgaatca ggatgcggtc gccggcgcct agatgttgtt
    16261 cgatgacgag ggcccgcagc cggtccaata accggcaggc ggccatggcg cggccgaggt
    16321 gatggtgttc gctggaccag agttcccgga tgcaggtgat gggtgtggcc agtttctgat
    16381 tcagggcttt ccggcaatgt tcgaccgtgg tggtcgtaaa gtttcccgcg tcgccgatct
    16441 gactgtcgag cagggtcttc gtggcctcat cgttggacag cggtggcttc atgccgcccg
    16501 gcagcggggt gatgccattc gtgccttccc gcatgaacga gaggagcgaa tccaggcctg
    16561 agatgccgcg tgaatagcct cgcttgagcc cgcccacttc atcgagccgt cctaagccga
    16621 agacatcggt gccggggatg gcgccatgca ggaacatgat cagccgcaca ccggcttgtg
    16681 acagcttctg gccgagctgg ctcatcgcct cctgccaggc cggagagtcg gcgtcgggcg
    16741 gcgtcgtcca gtccgcgtag gtcagccggt cgccgggatt tttgcgggag agctcgtcgt
    16801 gctgaaaatt gttctcgatc ggcatgggaa gattgtctta cccgaaacgg agcgcgaagt
    16861 tcaatggagg aacctcggat tcgacctgga aggtggttca gcgatgcgct gccgactcgc
    16921 ggccgttcga ttccatggat gctggtgcgg acaggcgagg cttcggcatg acggctgttg
    16981 cggagcaggt cttggcttct tcatcggtgt ggcgttccac gatgcggaca ccctgcaaat
    17041 attcctgaac gatttcctcc cgcacgactt cgatgtcctg ctcgacctcg cgaccggtgc
    17101 gttcccgcag gcggtcggtg gcatgttcct tcaccagcac gcggatttgt ttggcaatgt
    17161 cggccctggc tgccagttca gagacccgct gacagacgag tttccctttg cccaggtcgc
    17221 cctgcccctg gccggtcagc aactcctctg agctggatgt cgcttgcgca ggtctagcct
    17281 gggctgcgct ggtcggcgat tccgcccgtc cttgcatgtg gagatgttgg aaggtctggt
    17341 cagcgtgatc gcgcacggcc ggtggcgtca ggtctgcagc ggttgccggc gatgcgatca
    17401 gcagcgcgca gagcggccac catcctgttc taatgggtcc agccgtcagc gacattaccg
    17461 cgttcctccc tgatgagttc cgtttccggg gatctgttgc gaaggcgtcg tcgggatggg
    17521 caccggtttc ttcggtacct ggtagataat cgccggcggg gttccgcgag gtgcattgat
    17581 cgatggttgt tccgcagcag gagtggcctg gtcgactccc gatcgatagg tatggagcgt
    17641 gcgcgctgcc ggtcggccgg atggtggcgg gactcgtcga taggtcggca tcgtgccgag
    17701 gctcccctgc ggcagcggtg ctccaggctg tgccggtact tgaattgcgc ctggttgagc
    17761 cgattgttgc gggacgaccg cgtagcccgg caaaaatgga acggtatgtg gcacgggcgg
    17821 ccgccggggt tgcggcggcg ccgggggtgc tttcgttccc acggtctgct gcgcgatggc
    17881 caggtgggga ttgtcgggat tcagccgact cgctagccca aggagggcgt gaatttgcgc
    17941 gaggccggcc gatggagagg gttgtgcgaa cggagtgagt agcacccagt cggtccaggc
    18001 ttgggcgacc atcgcatcgg actccgcgcg attgagcaac tcgttccgtt cgtggctcag
    18061 ttcttggacc tgttcgggcg aggtggctac ggcgagggtg tcgttggctg cgtcgagttc
    18121 ctgctgcaac gattccacat cccgtctgag cgcaagcaac tgctcccgcg cgtcctcgtt
    18181 ctgccgtagg gtggcaatgg cctgggcgac ttcgtcggtg tttacctgtg ccgtcagctc
    18241 gacgcggacc acaacgaact ctccattcag ggcggtcgtc acgtgttgat cgaggaccag
    18301 caccattccc gcggtgtaac tgcggatttc atccagagtg acatccagat ctcgcacgac
    18361 cgtgacgctt tccagatacg aggcgacctg ctccaacgcc tgcttcttag cctgctcggt
    18421 ggcgagtctg atcccatccg ttttggtttc gtgatcgttc attcgatgtt cgcccgttcc
    18481 ggtgacgact cgaacctcag cttggcttgg actcgggcag gtgaggagca gggacagggc
    18541 gatagagagc aggccgataa aaaggtggat cgatcgagcc cgcggggacg tgtgggggat
    18601 gaagcggggg ccgctgtcga accggttttg gggcatcgcc tcaccaccgc ggagcgtctg
    18661 actgagtgtg gttgtcgagc gagttgtccc gaccggatca ggataccatc gatcccgctt
    18721 cgacgctatg agaggcgggg ttcgcgcctt gccttcgcgg aaaacagcat gctaaggtac
    18781 tttctcttcg cttctcaaaa gatcctatgg tggggcaggt tatgagtcgt attcgcagca
    18841 ttctgacagt cacggcagcc ctctgcatgg tcgtcggtct ggggctggca ctcgtggcgc
    18901 cctcgtccca atccgttgcc tttgcggcac gcggtggggg cgaagtggcc actcccgttc
    18961 aggcaggcat cacagaacat gtgattctct tcgtattgga agggttgggc caggaatcac
    19021 tcaagagtgg cgcgatgccg gtgttgtcgt cccttgtgaa ggacggggcc gtgacctggt
    19081 ctgctactgc agtggcccct gcgcgccggt tgccgacgat ggcttcgctg gtgacgggca
    19141 tgccggtcgc caaacatggg atcacctgga acgtctttga attcagccgt ggatatcctc
    19201 gcgcgccgac ggtgtttgat tatctggacc tgagcggcgg ccgggacagt gccattttct
    19261 atatggatga gtcgttgtat caactcgcga agccggagcc ctacacggat tatcaaatgt
    19321 gtggtccgct gcgggccgaa tgtaatcctg atcgtctggt cggctacgtc cgggattatt
    19381 tcaaaaaggc gacgagtggt tcagggtatg gtcatgccat tccagccttg ccgcatctcc
    19441 tggtggtcca tctgccggct cccgggcgtg tcggagagac gcagggctgg aaatcggttg
    19501 cctacaaaga cgcgttgaag gcggtcgata aggcgatggg gaccgtgctc gatctgtacc
    19561 gggaacatgg tttgatcaag cggaccacgg tgtttgccac ctcgctgagt gccttcgggg
    19621 agacgcaatt ctcggccggt gaggtctccg gtgagcaggc cggcaatgtt ccggtggtgc
    19681 catggattgc ctcaggtgtc ggcatcaagg ctgggcattc gattcgccag ccggtgtcga
    19741 ttatcgatac gggcgcgacg gtgatgcgcg cgctcggact cacgacgtat accgaatggg
    19801 agagccaccc ggtcgaagaa atcttcaaga ccgcattcgc tgctgctccc gtcagcccac
    19861 tcctgcaata gaggaccgat gcgttcatca tcgcacaaaa cattcgccgg gcggcaggtg
    19921 tttttctgga tcgtagccgt gacgtgtgtg ttggttctag ggccatccga gcggctcttg
    19981 gcggccggtc ctccccctgc ctatggcaaa gaacatacga ttacaatcga tccgactcca
    20041 ttggtgccgc aatcctggtg gcaggtgccc ggtgtgacac ccccgctctg ggtcttggac
    20101 ccggagacct cggatgccta ccggacaacc gaaacgcgcg agttgaagct caagccggga
    20161 caatacaagt tcatcagttt taccttcgac ttccccttca cggtgagttt ggacgggaaa
    20221 ctggaatact ccaagtcact cgatcaatgt gtggagggcc gcgggactca gaccctggtg
    20281 gtacggtgca agcgcaccta tcctcatggc ggtcaacgtg atgcctatta cgggaatagt
    20341 gaggtgggtg atggcacagg cactcgatga tctgctggat tccctcgaag atgcggacga
    20401 cgccacgcgt gaagaggcgg cgaaagccct cgccgagtta gcgaacccgg ccacgctgga
    20461 tgcgctcatc ggcgcctgcg gcgatgagtt ttggtctgtg cggactcgtg ccggctgggg
    20521 cgtggctaag atcggcggac cgaaggcgat tgaagcgctg atcgtgctct tcaacgaccc
    20581 gatcatggaa gtccgcaacg aagcagttgc ggccgtggtc tcgcttggtg cgggccagct
    20641 ggatcggctg cttgccgcga tgaaggatga gcggtggcgg gttcgggagc atgcggccaa
    20701 agcctgcggg gatctccatg attcgggtgc cgtcgatgcg ttgatatttg cctgtcggga
    20761 tcgcgacggg gctgtgaaga gtgccgccgc ggaagcgctg ggaaaaatcg gcgatccgaa
    20821 agccattccc gcgctggtga aattattccg tgattcatcc aagatcgtcc gtgaaaccgc
    20881 cggtattgcc ctggtggcga tcggacagcc ctccgtcgat ttgttgctcg agacgttgaa
    20941 ggacaaagat ttcgtcgtgc gttgtcatgc ggctcgcgca ttgggaggca tgacgacaga
    21001 ctatcaaatc ggccggagct gggtgcggga gccgcgtgtc gtcgatgcct tgatcgaggc
    21061 gttgaaagat ccggaccggg ccgtgcggga agatgccacg attgcgttgg gtatgatcgg
    21121 ggatccacgc gccatcgacg ggttattgga agcgatgaaa gacggtgccg tgaagcgcca
    21181 tgccatcgcc tcgctgggta tgatcggcga tcctcgagcc cttcctgccg tcttggcggc
    21241 gctcaaggga aaaggtgtgc gtcaggatgg tactccgacg cccgggtgca tcgtcagtga
    21301 agacgccttt atcaaggaag cagcggccac ggcgctcggc cacttccgcg atccgcgcgt
    21361 gattcccgat ctgatcatgc tgttgaaaga cggggtctta cgtgagaaag cggcggcggc
    21421 gctggtgctg atcggtgact cggctatcga accgctcatc tcttttctct atgatcccaa
    21481 agcgtcggaa gttgaagccg agggcgaacg cgtcctgtcc tacgcctcgg tgcggctgac
    21541 ggccaaagat tccctgcggc tacttgttgt tgaaacactg gaacatcttg ggtggacgcc
    21601 tccggatgaa gagacgtcgg tggactccag tcaggccgac aatcttcgtg tcgaccgccc
    21661 gctgggcgat atcggacggt tcggcccgag cggagacttt gcaaaaggat cggtgcggta
    21721 atcaccggct tcctcccccg gggtccgtcc ccagggatga gcatctccgg cgctcctacc
    21781 ggctactctc ctctgtttag accagatgcg cggggacggt tcgatgccag gtcgcacggc
    21841 cgaccctgcg ttgacccctc aaaaaatcct gtgttaaggt cgatggcctc accagaggcc
    21901 gaggtaccat ggccgacact gtcagtgaac agatcgcagc gctgtcggac gaagattggg
    21961 cgattcggga agaagctgcg accttgctgg gcagcctcaa cgacgcccgg gcagttctgc
    22021 cgctgacgaa gttgctccgc gatgccgatc gggctgtccg tgaggcggcg atcgggtcgc
    22081 tctccgcgct cggctccgcg tcggttcccg ctctggccgg ctgcttggcg gatccggcgt
    22141 tgcaggtgca ggaagcggct tcggccattt tggcctcgat tgctgatgcg cgggttttcc
    22201 gcccgctggt cgaggccctg ggcagtcgcg attggatcgt gcgcatgcat gcggcgaagg
    22261 ggctgggacg gatcggcgat cccgaagccg tcagcgtctt gatgccgctc ttgcaggaca
    22321 aggtcaaggc ggttcgtgag gaggcctcca gcgcactggc cgccatcggt gctgccgctg
    22381 tgttggggtt gatccaagcc ctggaacacg atgactggtt ggtccgcctc catgccgtgg
    22441 aggcgttggg taagctgaag tctccggatt ctgtcgatcc gttgctgcgt gccctgttca
    22501 atgagcggga ctcggcgatt cgcgaagatg tggtgcgggc cttgggtgcg atccgggatg
    22561 cgcgggcggt agattatctg gtgacggtga tgaaggaacc ggggttgcgg ctgctggccg
    22621 tcgaggcgct cggccatatc ggtgaccctc gtgccgtgcc tcttcttcgg cgggttgtgg
    22681 agggggtacc gctgggcgag cctcgcgata cggccacccc ttgtgccgac gggtggacgg
    22741 acgagatggc gaccatgggg atggcggctc gcgcgttggg gatgatcgcc gacggtgtgg
    22801 ctattccgtc tctgatcgtg gccttgcgga ataccattac ccggtctgag gcggctgcgg
    22861 ctttggccaa gttcggcccg gcggtgattc cgtccctgct cccgatgctg gcaaaggaac
    22921 aggacgagaa tattcggtat cacgttcgag agacgttgac ggctgtcggc tggcgtccgg
    22981 gacgggtctg acagggtatt agttcgtgat gtggtgaggt agccacggct gatcttcgcc
    23041 tctggcactc accgaatcag aaaacttcgc actcgccaac gaacacatgt ctaaagaaag
    23101 catcgataca ctcgtttctg agctgaccca cgaggaagag tggcggcgta tgcgggccac
    23161 agctgcctgc ttggccggtg ggcctcgggc ggtgcaggcg ttgacgcagg ccttggaatc
    23221 gggcgatgtt gctctccgcg ttgaagcggc ggcgatgttg tcgcggatca aggatccggc
    23281 agccggactt tctttggtcg gtttgcttcg ggattccgag gaagccgtcc ggcaagccgc
    23341 tttttcagct ctggaacaaa tggccggcaa tctggatgac gagacagccg cgggtctggt
    23401 gcgcaatctc cacgagtctc cagatgaaga tgtcaggagc cgggttcgtc agctcctggg
    23461 tgtgatcccc aacgccatca ctcccttgtg cgaaatgttg acccatccgg aggtcgacgc
    23521 gcagacgacc gcggcgacga tcctcgaaca cttgcttgat ccgcgctcgg cggacggatt
    23581 gattgatgcc atgtcctctc ccgcggttcg tgacaccgcc gtgcgtacgt tgaagaagct
    23641 gggggcgata cgggaccgga tcgatgcgac gtttaatgcc ttgcgtgacg tggagggggc
    23701 gagcgagcgt gaagaagccc gcatgtccac ggtcatggat ttactgggga tcgggcgacc
    23761 tgcggtcgaa attttgatcg agtatctgga agatgacgat tgggtggtgc gtgaagcggc
    23821 tgcggacttg ctggggaaga tcggggatgt gcgcgcggtc gagccgttga tgaagcggct
    23881 ccagttggat aaggataccg gagtgaaaga gtatgccgtg aagtcgctgg ggctcatcgg
    23941 agacccgcgt ccggcgcaat tatatatcga ggcgatcccg atcaggccct tgcgggtgat
    24001 ggcgatcgag gccttggcga aaatcaagga cgtcgaggtg ttgcgtccgt ataacgagct
    24061 gttcaaccgg ttgcggaccg accgggacgg catcgtgtcg tacagcgcgg gccttatcgc
    24121 ggacaaactt gcagcgcttg aaggcgaaca gccggtcgga caggaggaat cggacgatca
    24181 tgagtgatgc cgagcgaatt gcacaattaa tctccgcgct acgcgatgac aacgaggcgt
    24241 tgcgggacca tgcgatggcg agcctcgggc aaatgggcat cgaggccgtt ccacagctga
    24301 tcggcctgat ggccgatgaa gacgtggtga ttcgcgaagc ggccgcgacg gctgtggtgc
    24361 gaatcgggcc cgtggcgttc gaccagatgc tggaggcgct gagtgacgat gagtgggcga
    24421 ttcgcgagca ggcggcgaat gtgctcggac gtttccgcga tccgcgtgcg gtagatcccc
    24481 tcatggtggc cctgaaagac aaggacggag ccgtccggac ggcggccgtc tgggctctgg
    24541 aacggatcgg cgactcccgc gcgacgccgg gattgatcga ggcgctcggc gacgggacgg
    24601 tgcgagagga cgtagcgagg gtcttgaaaa agatcggtga taccagggcc gtcgatgcgc
    24661 tgatcggcgg actactgggt cccaattgga tggtgcggcg ccatgccgcg gaagcactcg
    24721 gcaagatcgg tgatccacgc agcgccgatg cgttgattca gtcgctccag gatgaggact
    24781 ggctcgtgcg tcggaatgcc gccgaatcgt tggcgcgctt gggggcgaag caggccatcg
    24841 aaccgctgct tcccctcctg gaagacgaaa acacgatggt gcaggagacc gtcgaaggtg
    24901 tccttgcgag tttgggatgg aaacaggcga cctcgtcata acccgataaa ggaactctac
    24961 tatggcagaa gaagcaccgg taaaactgat tcagatcgga ccaaagggcg ggaccaagaa
    25021 ggacgggttc aacctggtga cggaacgcgt ggtggccgtc aatcccgaag ccaagcagtt
    25081 ggaggtggag ctgcttgcgt acgacggaaa gaccgttgtg ttggatgtcg gcgatgaggc
    25141 cctggaagat ttcctcaaga tcaagcccgg cgatggggcg acgatccgtg tggttgagga
    25201 gggcggcaag cgcattgcca agagcttccg gattcgcgcc aaagacccga atgcggccaa
    25261 agccgatgcc atgttgatcg acctcaagga ttcccattgg ctcaatcgca aatatgcggc
    25321 ggaagtgctg ggtgagttga aagatccgcg cgccgttctg ccgctggtcg aggccctgac
    25381 cgacgaggtg ggcgatgtgc ggcagcgagc ctatgattct ttgatcaaga tcggcgggat
    25441 cgccgtcgcg tcactcgtac cgttgttggc ttctgaggaa gacgacgtgc gccagtcggc
    25501 gacggaaatt atccggaaga tcggtaagcc ggccgtcgag cccctcgcca cagccctggc
    25561 ggatgccgat gatcggctga aaacaagaat catgaaggtg ctcgatcgga tgggctataa
    25621 gccgaaggcc aaagagggtg ctcaggccga gcctgccaag ctgctcagtt agtgtgagcc
    25681 gtccggccgc cggttaggcg gccggattct tggtgggacg accgaccgag acgtggcgga
    25741 atccgaagcc gaccacgcgg tctgattcat aggtattgcg gagatccagc aggagtggtt
    25801 gccgcatgga tttcttcaac cgatcgaaat caagattcct gaactggttc cactcggtca
    25861 tgatgatcaa gccgtcggct ccttctgcga cgtcgtaggt gtcctggcag ggtaccatcc
    25921 ccggcaaaag tttcatcgac tcttccatcg aggccgggtc gtaggcgcga atcgtcgccc
    25981 cttccttcat cagttcgctc aagatggtca gtgacggggc ttcccgcata tcattggtgt
    26041 tgggtttgaa cgacaggccc aacacgccga gcgtttttcc cttcacgccg ccgcaggcgt
    26101 ccttcacctt ctcgaccatc cgcaggtgtt gttcgtagtt caccttggcc gcggcccctg
    26161 cgatctggaa cgggtacccc acgcgctcgc cggtctggac cagtgcagcc aagtccttcg
    26221 gaaaacacga gcctccgaat cccggcccgg catgcaggaa tttgttcccg atgcgattgt
    26281 cgaggcccat tcctttggag accatctgca cgtcggcgcc caccttttcg cagacggtgg
    26341 cgatttcgtt gatgaaggaa atcttgacgg cgagaaacgc attggaggcg tacttgatca
    26401 tctcagcagt tgggatgtcg gttacgacaa tcggggtttc cagcaggtac agcggacgat
    26461 agaggtcttt catgatcgcg accgcttgct cactgtccgc gccgatcacg acgcggttgg
    26521 gacgcataaa gtcttcaatg gcggatcctt cgcggaggaa ctccggattg gagacgatgt
    26581 caaaacggaa acggcccgtt tggttggcct tgatgacttc acgcaatttt tcgccggtgc
    26641 cgacagggac ggtggacttc gtgacgatga ccttgtagcc ggtcatgttt ttggcgattc
    26701 ctcggcccac ttcttcaaca taggacagat cggctgagcc gtccgatttc gggggcgtgc
    26761 cgacggcaat gaagatgacc agcgctttgt cgacggcctt ggcgacatcg gtggtgaaat
    26821 gaaggcggtc ttctttgatg cctttggcga cgagttcggt gataccgggt tcgaaaaacg
    26881 ggacttcacc cttctcgagt ctggcgatgc ggcgcgcatc cgtgtccata caggtgacgt
    26941 tcacgccgaa ttccgcgaag caggcaccgg tgactaagcc gacatagccc gttccaatca
    27001 cgctgatatg catggaagca tctcctcctg aagataagcc gtcgaaactg tgggtaagta
    27061 cgtgtgtaga gtcggtagta tagcaacgcg tacgaaagtg agcaaacata acgcaggttt
    27121 tggcaggggt gcggacgggg cgaattgcag attgccgcgc acatccagta gaattcggcc
    27181 ctcttcgaag gagaggctgg cgaatggcag gttcaggcgg gcccatccaa cgaccattag
    27241 cgatgatgat gcgtcctatc acgaggaccg tccatcccga tgacaccttg ttggccgtgg
    27301 ccaggcaact gcgcgacgcc cgcgtcgggg cgatgctggt cgctgatcac ggtgactatg
    27361 tggggattgt cagcgaggcc gatcttgtcc gaaaagccat ggccagcggc gcagacgcgg
    27421 aacaggtcat ggcccggtca gtgatgagca ccccggtcat gacgatcgat atcgcccaat
    27481 ccgcgcatga ggcgagcgat gtgatggccg agcggggaat ccggcatctc gtgattacag
    27541 aagaggggcg tatggtgggg atgatttcgg tgcgcgatct gcttcgctac ttcaagaatt
    27601 ggggaacgct ctaatatggc gtgcctcgat tccgaacggc actgttttgc ttctagcctt
    27661 tctgtggtcc tgtgttgatg atggcacagg gaccttccgc ctctcgcatg ccacgtctgt
    27721 ttctgctcat cacggcgctc gtaaccggtg ccatcgtcat ggcgctggaa attctaggaa
    27781 gccgtttgct ggctccggtg ttcgggaatt cgctgtttgt ctggggggcc ttgatcggga
    27841 tcatcctggc ggccatgagt tcagggtatg cgtttggcgg ctgggcgtcg gatcggtatc
    27901 gtgtcgcggc agtgctggcc tggctcctgc tcggttccgg cgcctggacg ttgctgatgt
    27961 cctggatggg gcaggttgcc atttttaagg ttgccaagct tgtcgaggac ccgcggtggg
    28021 gccccagcct tgcggcctgc gtgttattgg cccccccggc attcggattg agcggcgtca
    28081 tgccggcatt gcttcggttg gccgtggtcg atatggggta tctcggccgc cataccggca
    28141 gcatgattgc gctgtccacc gtcgggagtc tggctgggac ctgggggacg gcgttcttcc
    28201 ttctctcctg gctggggacc cagacgctcg tcgcttcatt gggtgtgctc cagcttttgt
    28261 tgggtttgtt gtggttgcag caggggagcg gaaaggtcac gaaagtgacg ggtttgttgc
    28321 tggccggtct gcttattttg agttggcaat tattcgggca ggcggctccc gttgccgggc
    28381 tggtgtacca ggaggatagc ccttatcagc aggtgcgtgt tcgtgacgac gatctgtttc
    28441 gttatctggt cctcgaccgc acctggcatg cggtcatgtg gcggtccgac cctgccacgc
    28501 tctttcttcc ctatagccaa ctcatggttg cagcggtcgc gttgaccccg gaccccaaac
    28561 gcgggctcat tttggggcac ggtggtggat ctctcgccaa gtggctggcc caggtctggc
    28621 cggagttgga gctggatgtc gtcgagtttg acccggtcgt cgttcggatg gcccaggagt
    28681 attttgagta tcaccccccc gcgaaccatc atgtcttcgt caaagatgcc cgtgtgtttg
    28741 tgcgcgatac aggggtgaag tacgacgtca tctgggtcga tgcgtttgcc cggcacctca
    28801 tcccctttca tttgaccacg gttgagtttt tctcagaact ccggagtcga ctgaatccca
    28861 atggcgtgct ggccctcaat ctggcgtcat ccggcgaggg tggagatctc cagcgtgcga
    28921 gcgcggtcgt gcagacgttg aagacggcgt ttcccacact cgagtccttt ggagtgaaag
    28981 ggccttggcg ggcgcatcaa accacggcgg agaatttgat ttttttcggc ggcggtccca
    29041 tcgatactat gccgtacgac gaggttgtga agcgtattca atcgcatgtc gaagctcggc
    29101 gactgccatc cgaagcgact gccttgttgg cggcgcgacg cacccagccc tggtcgcctg
    29161 gcctgacctt gaccgacgac tatacgccct acgatttgct gatcggatcg catgcggtag
    29221 aaatgtcgcc ggagggaaag gctttgccgt aaacgtatga aattatgaat ggaatgaagc
    29281 ctgtcgagtc tttggtccgt aagacatttc gtggggtaac taggttcgct tcacgtccaa
    29341 gaactttctt gcttcctttt taatccctat gctataaggt gtccctcatc agcctattat
    29401 ttaggacatt ttcgagtagt agaggctagg ctcctgggcg gagtgtctag cagatggttc
    29461 agagcgcggt ggtcattatt caccaaccta atcgcatctc ttcttaatct ctttaaggag
    29521 gtaggtcatg ggtaagacga tggtagcagt gttttttggc ctggtcatgc tggtggctgg
    29581 ggcatcaggc gcatatgcgc ttcaagctgg tggtgtcgaa atcggtgatt tggataccgg
    29641 ttctgtcgtc ggtcagccgt ttaaggaatt ggtcgtggat gcggaattgt acgccgtcga
    29701 aatcggcaac atgaaggcgt ggtatccgcc tgtcacggtg atcgacttta aagttcgtcc
    29761 gggccgtccg gtcgttttga aagtcacgaa caactcgact gcagagcatg ggttccagat
    29821 gaccgatgct gcgaattccg gttccccgtt cgtgatgaag gctgaagtcg tgttgaagcc
    29881 gggagaaacc aagtacatcg gtgttcccac cagtgacatg ttttatgcca cccagggtaa
    29941 cacgttgacc tatcgctgcc atttgcatcc ggcccacgtc ggtggcaaga tcttgatgat
    30001 caagtagtcg acgtttcaaa tacgtgtagt gcaacgcccc atctgcagtg atgcagatgg
    30061 ggcgttgttg tatcaggcgc atcgccgact ggtatcgata caatgtttcc ttcctaggct
    30121 tcctgctcct tttggcattc ggcttcggcg tccaaaggaa tattctgaat gcctctgagt
    30181 ctcccgtcta cgatgagtag caaaaagcgg cttgctgtgg ggacatcctg ccgccgctcg
    30241 gtgacaatgg cgttcatgtc cacgatcatt tcgtcgccgt cccattgcgt gtcattgacg
    30301 atccagaagt gattggctgc atccggaaac tgtttcgtga cgaagctttc gatgatggtt
    30361 ccgacgtctc cggtatcggc catggtttgg acgggaagca gggcgaggcc gaggctggcg
    30421 aataagatga ggcgacttac gcgtgtcatg agcacctccc gctgagaggg tgacgaatgg
    30481 gcggagtgca gcaaggcctt aagggtgggg gcctgcgcag gccgcctcgc tcttaaagtc
    30541 tacagctgtt ggccagtgaa agccagggag ttatctcttt tgcagcgcag aaacgccgaa
    30601 ggctcccgga ggagccttcg tggtgtcacc tataggagaa tgacgtattc tgactagttt
    30661 cgaaccccgc tccacacctt ggccggatca atttctttat ccggattcaa ggtcttggcc
    30721 ttgtcgagca acacctcggt ggtttcgggg tagaccggat cggcaatgca gcaattgtct
    30781 accgggcaga ccgccgcgca ctgaggttcg tcaaagtgcc cgacgcattc cgtgcaacga
    30841 tcatgagtga tgacataaat gttgtcaccg actccctgac cgtcgccgac gtggttgccc
    30901 ttgccttcag cgtcgctgcg ggtttcaaag atcgcttcgt tgggacattc tggcaggcat
    30961 gccccgcagg aaatgcactc atcggtgatc agaagcgcca tggcctgtgc ctccttctca
    31021 gtttctttaa tgacggataa gatgttgaca atttcgtgcc gccgcgacca tagttcgcgg
    31081 cgaaacaggt ccgcattcta tgccgccgtt cttcggcaag tcaacagagt gaggctcggg
    31141 aagaaggcct agcactcgga aagtcatggg gctttgggcc caatgtacat gcggctctgc
    31201 gcgtgtgcga tcacatgagg atgcatccat gttcgaccgc atttggttat ggtgaggcgc
    31261 gcgagtgcgg ctgagtcgga gcagcgtcgc aagaaaattg tgacgttcgt gctcttggtt
    31321 attttgttgg tcagcgtgag cctgtttctt ggggggccga aggagtccga gctcatcatc
    31381 gtggaatttc ccaacggaaa gaccatggaa acggaagtgg ccagcacccc tgagaaattg
    31441 ttgttcggtc tggcgtttcg agaaggattg ccagccgata cggggatgct ttatatcttt
    31501 gagtcgacgg gtttgcatcg cgttcagact cggcagtttc gcatcgccgt cgacatggtg
    31561 tggattgatg agagccacca cgtggtccac atcctggaac aggtgccgcc ctgcgagaag
    31621 gatccgtgtc cactctatgg gcctccgccc gaaccggttc gctacctcat cgaggctgaa
    31681 gcgggctctg tccagcgcgc aggcatcacg acggggatgg aactcaagtt tacgcttcgt
    31741 atgtaaagaa tcggagtcgt ttatggaagg gtttcagtgc gtggccaagg tcgaagagat
    31801 tcctcctggg caggtcaagg ttgtgaaggt caacgagcgg ggcattgccg tatttaatat
    31861 cgacggtcaa tttcacgcga tctacaattc ctgccctcac gagggcgggc ctttaaataa
    31921 gggccgggtg aaggggcacg ttgtctcctg tccctggcat gacctggcgt tcgatgtccg
    31981 aaatgggcaa ggcacggatg gcggcggcta ctgtgtcggg agctatgatg tgcgggtgga
    32041 agacgggcag gtgtttgtcg gggggcggcg caaggtatag tcgggcgagt gattgcctcg
    32101 gggcagcatg agaatagtat gagtccatta agtaaggcag ttcccaccgg tgcagagcag
    32161 gagccgggcg ccaagacgct caaggcgaga gtgggggcat cggttcacat tcggctgtgg
    32221 gaggatcgca cccgcgggga gttgtgggtt ccgtcctacg acgcgaccgt catgcctctg
    32281 gttgaggacg attttcttcg cactgccagt aacaatgcgg tcgacgcggg gcagcgtacg
    32341 tttgaattca ggcctgtggc cgcaggaacc caccggttgg tgtttgaaaa gcggatgggg
    32401 tggaagttca cggctgagga ccgacagatt ttttacgtga ccgtatcctg aggttcgaaa
    32461 cgtgctgaga aatgagtcat gcctgacatt gcgtatgtga atggtcgctt tggtccgctg
    32521 gctgatgcgg tcgtcagtat tgaggatcga ggctttcagt tcggtgacgg ggtctacgaa
    32581 gtcattcgga cctatcgtgg acagcccttc gccattgagg agcacctggt tcgattggag
    32641 cggagtgctc aggcgctgca gcttgcaatc gggcaaacga gggctcagtg gagttccttg
    32701 attcgagaag gcctgcggct gagccaattc cctgaagcca aaatttatct ccagatcact
    32761 cgcgggcagg ctccccgcga tcatccattc cctgtatcgt ccgcccccac caccgttctg
    32821 acctttcgcg aactccaccc gctggatgtc tccgttcgca acgccggggt tcaggctatg
    32881 tcggtcgagg acattcgatg gggacgctgt gacatcaaga gcgtcaatct tctggccaac
    32941 gtcctggcgc ggcagcgagc caaggaggcg ggcgtgtttg aagcgattct ggtccgagag
    33001 gggacggtca cggaagggtc ggtcagcaat gtcatggtgg tgcggaacgg cgccgttcag
    33061 acggcgccgg aagggcgcag gattctgtcc ggggtgacgc gcgcgatcgt tctggatctg
    33121 gcacgcaaag aaggtattcc ggtggcggaa tcgttcgtca ctcgggaaga attacttgcg
    33181 gcgtcggagg tatttctgac aggaacgacc gtggaggtat tgcccgtggt gcgtgtcgac
    33241 ggacgtgcga ttgggggcgg atcgcccggc cccgtttctc agttattgtc tcgccgtttt
    33301 gaagcgctga tcggctagcc gccccttgct ttcactacta tggcatggta aggttctcgc
    33361 gctgtaagtg ggccaaggcc cacttttttg tttgagcaac tttttctatg agtgacgcgg
    33421 gagcagtgtc ggcagggaag ccgtcggtga aacgcgctga ggcgctgaac cttcgcatcc
    33481 aagaaattgc cgctcctatt ttgcagtctc atggtctgga gttggtcgag gccgtctgcg
    33541 tcggccaagg gcccaggacc gtgattcgtg tgtttatcga caagcctggg gggatcaccc
    33601 tgacagactg tgagcaggcg catcgctcgt tgagcccggc gctcgatgtg attgatccat
    33661 tcccgcatgc ctatacgctg gaaatttcgt ctcccgggct ggatcgtccc ttgcggagcg
    33721 tgcaagacta tcggcgtctg atcgggaagc cggtcacact caaattgtgc gaacctattc
    33781 aggcgcagtg gcggctggtt ggcgcagtgg tcgaggtgga cgaccggggg gtgacccttg
    33841 ccattcagca gaagaaaacc acggagacaa tccgagttga gtttgatcaa atcgctctgg
    33901 cgcggcggaa ggtggagttc tcgtagcagc aggtgccagg gtggtgtgtt aaccatgagg
    33961 tgtgagctat gaaccgagag ttgatcgccg tcatcgatga aatcggccgt caaaaaggga
    34021 tcgataaggc cagggtcatt ggcgcgatcg agtcagcgct tcaaactgct gccaagaaac
    34081 gattcggcca ggccgaaaac atccaggtgg aaatcgatcc gaagaccggc gaaatctcgg
    34141 tcgtttcaaa gaaaatcatt gtcgatacgg tggccaatcc gaaagcggaa atctcgctaa
    34201 aggaagcgcg cgaatatgac gaaggcgcgg aggttggcga tgagatcggc tcattgatcg
    34261 agatggacga gttaggccgg attgcggccc aaacggccaa gcaggtgatc tttcagaaag
    34321 tgcgcgaagc cgagtgggaa gctgttcaga aggagtactc gacccgtcag ggtgatttgg
    34381 tgaacggcat tattctcggc atggagcgcc gaaactttct ggtcgatctc ggcaagaccg
    34441 aagcgattct tcccattcag gaacagatcc cgcgcgaaac ctatcgtcgc ggagaccggg
    34501 tgaaggccct gctcttggag gtgcgtcgca cccccaagga cgtgcaggtc attctttccc
    34561 gcagccaccc gcagtttgtg gcgaagatgt ttgagctcga agtgccggaa gtcggcgaaa
    34621 agatcgttga aatcaagtcg atcgtgcgtg agcccggcga tcgtaccaaa attgcggttt
    34681 cctcgcggga caaggctgtc gacccggtag gggcttgtgt cggcatcaag ggatcgcgtg
    34741 tgcaggctgt ggttcgtgag ttgcgcgggg aaaaaatcga cattattacg tggacacagg
    34801 atccgcgtgt ctttatcgcc gaggcgctga atcccgccac gatcgagaag gtcgggatcg
    34861 acgaagaaaa gaagtcggcc ctggtggtgg tggccgattc gcagttgtct ctggcgatcg
    34921 ggaagaacgg gcaaaatgtg cgactcgccg cgcgcttgac cggttggaag atcgatatca
    34981 tcagcgcgac cgaatacgag aaagaaaagg cggagcggga tcgggatatc aaggcggccc
    35041 tggcagagga aaccgaggcg cagcgccagc aggatgaagc gcgggctgct gcgcaacagg
    35101 cggagtgaag cggttcgata ttcacgggct gaatggcaat tatttaagcg ggtggagctg
    35161 agcggtactg tatgcgcgtg tacgaattag caaagcagct ggggatggaa aatcgggagc
    35221 tgattcctga actgaaacgg ctcgggattc cggtggcgtc ccacagcagc gccttggatg
    35281 acgattcggt tcgcgttgtc ttggagaagc tgagctcgaa ggctcggggc ggtgaggctg
    35341 tctccggcgg gcatgaggcg aagaaaatgg ttcgctcgtc gaaagaaccc ggtgcgtctc
    35401 acgagagggg acacgcggcg gcgcacgaag agcccccgaa gccggacaag aaacggattc
    35461 tcatcaagaa gaagagggaa gaaggtgcgg aggatgttgc ggcctctctg gcggcggcag
    35521 aagcggcgtt tgcgcccgtc gtccctgcag gaaccgaagc gtcggtggta gcgccggcgg
    35581 ctggtcctga cgcagaagcg attgaagccg ccccggttga acctgcccca cccgaagaat
    35641 tagtgtcccc accgcctgtc gtgaccgttt cggaggagcc gctcgtcaag ccggtccagg
    35701 ttccccaggt cacggctccc ccgctcgatg cgctggcgac ggcaaagaag aagagtgcgg
    35761 ccacggaggc catggagagc gaagcggcgg ctcgagaaaa attgaaaaaa gccaagaagg
    35821 ctccgcggac ccgtgaggaa gatgaagcca agtttaagaa cgacgccact cgatggggag
    35881 atctgcgggc aatcccggtg cagcggcgcg aagaccgatc caagcatatt caccatgcct
    35941 cgccaacgga agtcaccaag ccgagaaaga aaagcgtgaa attgagcgcg ggagtgagcg
    36001 tcaaggaatt cgcagaactc atcggtcagc ggcctgcgga cgtggtccgg aagctgatgg
    36061 agatggggca gatggtgacc ttcaatcagt cgatcaatct ggaggctgcc tccttgatcg
    36121 ccgaagagta tggcaccagg gtggaagtgt cgacagaaaa ggttggcgaa gcgttgctgg
    36181 aagaagccgc ccaatcatcg ggtgaggagc atgcggtctt gcggcctccg gtcgtcacga
    36241 tcatggggca tgtcgatcat gggaaaacgt ctttgctcga tgcgattcgc ctgacaaagg
    36301 tggcggaagg tgaggccgga gggattacgc agcatatcgg cgcctatatc gtcggagttc
    36361 gcggtaagca ggtgaccttc ctcgataccc cgggccacga agcctttacg gccatgcgtg
    36421 ccagaggcgc gaaggccacg gacattgtga ttctggtcgt ggctgccgat gatggcgtca
    36481 tgccgcagac ggtggaagcc attcaccatg ccaaggctgc cggagtgcca ttgatcgtcg
    36541 ccatcaataa ggtcgacaag ccgggagcca atatcgatcg tgtcaagaat gccctgaccg
    36601 agcatgggct ggttcctgag ccctggggcg gcgacacgat tatggttgag gtgtccgcga
    36661 agcaacggac cggcctggac cagttgttgg aaatgattct tctgcaggcg gaagtcttgg
    36721 agttgaaggc ggatccatcg cgcatggcca agggcctggt gatcgaggcg aagttggacc
    36781 gcgggcgtgg accgatcgcg acggtgctgg tgcagagcgg gacgctccat gtcggggatg
    36841 catttgtagt cggcaatttc agcggccgcg tgcgcgcgct cgtgactgac acggggagca
    36901 agacgactga ggctggcccg tctgttcctg tcgaggtgat cggtttgccg ggcgtgccgt
    36961 cggccggcga tttgttcacc atcgtgaaag atgaacgggt tgcccgtgag attgcgcagg
    37021 agcgggcgat gaagcagcgg gcggcggatc tggctgggcc ggcgaaggtg agcctggatg
    37081 atctgtttgc caagattcag gaaggcaatg tcaaagaatt gcccatcgtc atcaaggccg
    37141 atgtgcaggg gtcggccgag gccttggctg ccgcggtgga aaaaatgccg gccggcgcag
    37201 tcaagttgcg tgtaatgcat accggggtgg gcggtattac cgaaaccgac attctcttgg
    37261 cggccgcgtc caaggccatt gtgatcggct ttaatatccg tccggagccg aaggcggcgg
    37321 cgttggccga gcgggagggc gtcgatgtgc gcctgtacag cattatttac gatgcactca
    37381 acgatatccg cgccgcgatg gagggcttac tcgaacccac gctcaaggag cgtgtccttg
    37441 ggcgggctga agtacggcag atgtttacga tccccaaggc cggtctggtg gccggctgtt
    37501 acgtggtgga tggcgtcatc tcccgggcca gcgtcggcgt gcgcgtgatt cgggatagtg
    37561 tggtggccta tgaaggcaag ctgggctcgc tccgtcgctt caaggatgat gtgcgtgaag
    37621 tgcagcaggg gtatgagtgc ggtatcacgg tcgaaaactt caatgatctc aaggccgggg
    37681 acattatcga ggcctacgtc gttgataaga ttgccgccaa gcttgagcct gcgaaccgtg
    37741 gcacgtctcc gcaaagtcat cgggcatgac cgtcgggctc tgcacggttg agttgttcat
    37801 tcccgacggg cattcgttga aagagaagcg ccaggtcctg caaagcctga agacccggtt
    37861 gcgagacaag tttaatctat cggtcgcgga agtcggagac caggatttgt ggcagaaggc
    37921 aattttaggt atggcctgcg tggcgaatga atccgcgcat gtgaaccagg tgttggatca
    37981 agccgtgaat ttgattcagt ccgtgcctgt gatccaacta gtacgctcgc gaatcgagtt
    38041 gctgtaaggc ggcgtcatga ccaaatcgac gtacagccgg gccgatcgtg tggcagacca
    38101 gatccgcatg gaagtggccg acattctgat gcggaagatt aaggatccgc gtgtgcgctc
    38161 ggtcaccgtg accgatgtgg agttgaccaa agatcttcgc atcgcgcgcg tctttgtgac
    38221 gaccatggag cagaataagg atgagcggtt ggtgttcgac gggctggcga aggccagcgg
    38281 tttcgttcgg gcggagttgg ggcggcgtct ggcattacga tacctgccgg agctgatctt
    38341 tatgaaagac atcagtggcc ctagagctga ccgagtcctg gagctgttgg atggactgca
    38401 ccgcgatcaa tcccacgaag agatgttggc agagcccccc cacaccgatg tctagtaaat
    38461 gaccatgatc gcctcgatgg agccacgcgc gactactgtg cttcaggacg gagtgttgaa
    38521 tgtccgcaag gaagccgggt ggacgtcgca cgatgtggtg gctcgcattc gtgggaagtt
    38581 gcgcggcatg aagttgggcc atgccggaac gctggatccg gcggcgaccg gcgtcctgcc
    38641 gttgctcgtc gggcgtggaa cgcgcatcgc tgaatatttg ctggaatggg acaaaaccta
    38701 tctcgccgga ttgcgcctcg gtgaaacaac cgacacgcaa gatgccaccg ggaccgtgct
    38761 cctgcgttca ccgattgagt cgttgtccga agcccgcatc cgtgaagtgg tagctgggtt
    38821 tgaggggcgc attcagcaac tccctcccat gtattctgcg gtaaaagtcg gcggggttcc
    38881 gttatacaaa gcggcgcgag ccgggcggga ggtctcccgg caggttcgtg aagtgaccgt
    38941 ctttcgcctc gatatcgtgg atgttcagat tcctgaagtg acgctccggg tggtctgttc
    39001 caaggggacg tatatcagga cgctctgcgc ggatatcggc gacaagttag gggtcggcgg
    39061 tcatatggcg acgttggtcc gcgagcgcgt ggggccactg acccttgagc aggcgttgac
    39121 ggtggacgaa gtcgaatcac gcttggagca aggcacgctg gcggcgtcga tgttgacgtt
    39181 ggatcaggcg ctggtcggac ttccggtttg tatcgtgggg gcggcgacgg ccggccgggt
    39241 cctgcacggc atgccggttc ctgcctcaga agtgctggcc tggcatggct tgtcttcgcc
    39301 tctctctgag ggggcgaatc gggccattgg catcaaggac ggagcggggc ggttattagc
    39361 catcggcacg atgccggacg gcatgaatgc tgaggcgccg ggggcgaatg cgcagtcgat
    39421 tgctgtatcg aaagtattgg tcacagaaga atcacagggt ggtagtatcg cgaactcaat
    39481 agaggagtag agacgtatgg cactggtaaa agaagtgaaa acggaattgg tcaagggttt
    39541 ccagcagcat gacaaggaca ccggatctcc ggaagtgcag atcgcgatcc tgacgaaccg
    39601 gattacgtac ttgacggaac atttcaagtt gcacaagaag gaccaccatt cccggcgcgg
    39661 gttgttgaca ctggtcggac gtcgtcgtcg gttgttggac tatctccgaa aagttgaaga
    39721 agcccggtat cgcgccattc tggagcgact gggtattcgt aagtagtcgt gtgagcgaat
    39781 cggctgccac cttcgtgggt ggcggccgaa ccatttgttg tccccgcagg tgaacactga
    39841 aacaagttca acggcacggg tcgacattca atcgagggca ggcgcttgtc gttggatgtg
    39901 tagatggccg gttgaactta tctctgtggg catctgtggg atttaaacgg aggagaccat
    39961 agatgaaaca tgttgtagac attgagctgg cagggcgtcg gttgacgctg gagaccggtc
    40021 gtatcgcgaa gcaggcggac ggcgccatct gggccaccta cggtgatacc gtcgtgctcg
    40081 caacggccgt tgcgtcacaa aatgccaagc cgggagtgga tttccttccg ctgaccgtgg
    40141 attaccagga aaggacttat gcagccggta agattccggg cggctatttt aagcgggaag
    40201 gtcggccttc ggagcgggaa gtgctgacca gccggctgat cgatcggccg cttcggcctc
    40261 tctttccgga agggtattac ttcgagacgc aagtcattgc ctcggtattg tctgccgata
    40321 agacgggcgt gtcggatgtc atcggcatca tcgcggcatc cgcggcgttg gcggtctcgt
    40381 cgattccatt caacgggccg atcgcaggcg tgaaaatcgg ccgcgtcaac gggcagttgg
    40441 tggtgaaccc cgatctggaa accctggaaa ccagcgatct tcatctcgtc gtggctggga
    40501 ctgccgatgc ggtgatgatg gtcgaagccg gggccaatga attgccggaa gcgactatgc
    40561 tggaagccat tgagttggcg cacagcgaaa tcaagaaaat cgtcgcgaag atcgaagaat
    40621 tgcgcaagtt ggccggcaag ccgaaacgga cggtggtgca agagcccatc gatgccgcat
    40681 tgaccgaaca ggtgcgtgcg ctggtggccg gtccgatccg cgaggccatc ctcatcccca
    40741 ataagagcgc aaggcaggag cgcctggatc aggtgttggc cgagacggtt gcgaagctca
    40801 aatcggacga gccgaatcgg gaccggcatg tcaagatcat tttccatggt ctggagtaca
    40861 ccgaagttcg caacatgatt ctcgaaaagc gggtgcgcgc cgacgggcgt ggtccagcgg
    40921 acattcgtcc gatcacctgc gaagtcggtg tgctgcctcg cgcgcacgga tccgcagtct
    40981 tcactcgcgg agaaacgcag agcctggcgg tggtgacgct gggtacgacc gacgacgagc
    41041 agcgcattga tgccttggag ggcgaataca tgcgcacctt catgttgcat tacaatttcc
    41101 cgcccttcag tgtcggcgag gcacggccgc tccggtcgcc gggccgccgc gaggtcgggc
    41161 atggagctct agccgagcga gcgctcaagt cgattcttcc cggcaaggat aagtttcctt
    41221 acacggtgcg aattgtatcg gaaatcctgg agtcgaacgg atcctcttcc atggccacgg
    41281 tctgcggcgg tacgttggcg ctcttggatg cgggcgttcc gattaaggag ccggtggcgg
    41341 ggatcgcgat ggggttgatc aaggaaggca atcaggtgct ggttctgtcc gatattctcg
    41401 gattggagga tcacctgggc gatatggact ttaaggtcac ggggacgaaa aacggcgtga
    41461 cggcgcttca gatggatatc aagatcggcg gcatcacctc cgaattgatg cgcgaagcgc
    41521 tggctcaggc gaaggcggga aggctccaca ttctcgggtg tatggcccag gcgttgaccg
    41581 aaccgcgcac caagctgtca gccttcgctc cgcgcatatt cccgatgaag atcaagcaag
    41641 acaagatccg cgatgttatc ggccctggtg ggaagatgat ccgcagcatc atcgcggaga
    41701 ccggtgtgaa gatcaatgtc gaagacaccg gcgatgtgac gattgcctct tccgatgaag
    41761 catcggcaca gaaggccatc gatatgatca agcgcctgac cgaggaagtc gaggtcggca
    41821 agatctatct cggaaccgtt cggaagatca tggatttcgg cgcatttgtc gaagtgcttc
    41881 ccggcacgga cggattggtg catatctccc agctggctca tcatcgcgtc aaggcggtca
    41941 ccgacgaggt gtcagaaggc gatcaggtga tggtgaaggt gctggaaatc gataagcagg
    42001 ggaagattcg cttaagccgg aaggaagcca tgccggcacc ggctggttct cctgccaccg
    42061 aaccaacgcc cgcgggataa tcgatcgtgt atcgcaagat cgttctcgac aaccggttgc
    42121 gcattgtggc cgaactgctc ccgacgttga agtccgtcac gatcggcata tgggtcaacg
    42181 tcggatcgcg cgatgagcag ccgggggaag aggggctgtc tcacttcctg gagcatatgt
    42241 tcttcaaggg aacgcgcagc cgcacggcga cgcagatttc ccgcgagatc gatgcgctgg
    42301 ggggtgagat gaatgcgttc accacccgag aaacgacgac cttttatgtg aaggtgctgg
    42361 atcaacagtt ggaggcagct ttggagctgc tctcggatct gttctatcgg tcccggttcg
    42421 agtcgaaaga agttgaaaaa gaaaagcagg tggtgctcga agagatccgg atggtccaag
    42481 acgatccaga ggatctggtt caagagcttc acatgaagca cacgctcgga agccatccgc
    42541 tgggcaggcc aatcctgggg caagctccga gaattcaggc tcttgggcgt aacgatctgg
    42601 tgtcctacgt tggttcgcac tatgatccgg agcgcaccgt cgtcgcggtg gccggcaact
    42661 tcacctggcg ccgcctggag cagttgttgg cgcggtattt ttccgactcg cacaagggtg
    42721 tggccgctcg gccaagccgt cgcccgcctg aagtgaaggg tggggtgctg gtcaaacgta
    42781 aggctcttga acaggtccat ctttgtttgg gccttcaggg tctgagtgcc gggcataagg
    42841 atcgatatgc ggcgcatgcg ctcaacgggg tgctaggcgg gagtgtaagc tcacgcctgt
    42901 ttcaggaggt acgggaaaag cgtggcctcg tgtactcgat ctattcgttt ctgtccactt
    42961 actccgatgg cggaatgacc acggtgtatg ccggtacgcg cccgaaagag gttgagcgtg
    43021 tcgtggaagt tgtctgccgg gagctgaaga agctccgcac gcatggaatc gatgcgaagg
    43081 atttggcccg ggtgaaaaac caaatgaagg gcagtctgat gctcagcctg gagagttcgc
    43141 acagtcgcat gagcaagttg gcgaaggacg aactgacgca aggcaatcac gtgtcgctcg
    43201 agcagatgat tgcggaaatc gatcgcgtga cgaccgatca ggtgtatcgg gtggcgcaaa
    43261 cgttactgga tcagcgctgt ctttcaatta cggcacttgg cccgattccg accaagagtc
    43321 tgcagtcgtt tgcgtcataa ccggtcaatt gcggggtacc cgtaggcggg gcgcaagggc
    43381 tggtcgggca atgagtagcg gtgatttgag cgccattgca ggactaactc tttgctacgg
    43441 tactcatttt agggcgcgaa tcatggctgg atttcggaaa aacattttct tgacacgttt
    43501 cgtggattta agtaccatgg ccgcgttttc atcatataag tctgaattta tttcggctgc
    43561 attacagggg aaggtaagca gagagaaagg gggtgtgcgt cgcacttcct cctcctagcg
    43621 cgttctctgg aattttgttt ttttctgagc tctttttttg gatactgtga ttcaacggtt
    43681 ctctggtcat catttttcca aggagggtag ggttatgaat agggtcggaa ttctagccgc
    43741 acttgcagtc tcctccgttg tcggcttgtt gaccgccggt gttacgttgg cggcggatgc
    43801 tccagccggg ggcggaccga gcgaagttac cacgggcagc ggaaagtttt ttaagggcga
    43861 gaccaccaat accttgaagg gtcgggtgtg gtcgcaatgg gcagacaatc ctgataccga
    43921 attgctcttc ggcatccagt actggaacac cggtgatccg gcttccggtg agggcggcgg
    43981 ccgttcttca acgcaaatgg atgtgaagcc gccggatcct ttgtttacct gctgcggctg
    44041 gggcttcacc ggtggtacgg acaagaacaa tccttacgcc ggctggcacc acgcgcaaac
    44101 caccgttcgt ttgtctgtta aggataagag tttgatggat caaatcatca aggcatcgca
    44161 ggaactcgtg gcgatggaag taactcttga tggtcggacc attaccggat tcaaggtctt
    44221 gaaatagaat ttatacgcga ggcgacattc ttgagacttt tttgtcttct ttaaggagga
    44281 tgtgattatg aagcttcaaa agcaggggtg gacctttatg gctgccgtgg ctttggtggt
    44341 gggttttgcc tccactgcgc tggccatcga ggcgttccag gagcgattcg agtggggaga
    44401 tctgagcaag cccactacgc tgcaggggcg tgtcatcatt ctagatccat acgatgaagc
    44461 ggtctggctc aacatcgctg tgttcggcgg caacgcagag agcggtttat tttggcagag
    44521 aattcaccca gggaagaacc tcaagttcta tcccgcggac aagaatgtgt gggagcagct
    44581 gaagtccatg gcgcgtccgc attccggacc ggctgcagcg aaggaagttc cccctggatc
    44641 cccgaccact ctggtagagt tcgtggctca ggaagtcgag caaaatcgcc gcgtgatttc
    44701 gtcagtcaag aagttggccg acaatgccgg cgaaatgggt aagccaaaga gcatctcctc
    44761 ccttcgcttg aaggaatgtg aagggaagac ggaagtggat gctccttgcc acgccgcgaa
    44821 gacgttgctg aacacgtccc caaagactcc ggatggagcg aatttgccgt tgcaatatga
    44881 tgtgttgttg cccaatggca agccgcttgc caacctgatt ccctggactg ccaagtacaa
    44941 tcagggtggt ggacatcact gagtagtaga aggccattgg cctgaagtaa gaaggcggca
    45001 ttccgaaagg gatgccgcct ttttatttgc gacgtcgagg gaggaactgt ctaggccggg
    45061 atgtgagcta ttcctgtagt agccgttgtt gcagttcagc aagtcgattg agcgcatcga
    45121 ggggggtcat ggagaagaga ttcatttggc gaacttcctc aagcatagga tgaggcgcag
    45181 ggagcgtggc atcaaattga agcgactgct ggttctcctg cactgaccgc ccggatgacg
    45241 aggaggactc aagttgagca aggacggcct tggcccggtg tattaccgga tcggggagac
    45301 ctgccagttc agcaacatgg attccgtagc tgcgatcggc gccccctcga ataatctttc
    45361 gcagaaacag caccttcccg tcacgttcct gtaccgcaac gcaatagttc gtaatccctt
    45421 cccgtagact ctccaattgc gtcatttcat gatagtgagt cgcgaagagc gtgcgcgcgc
    45481 cgagacgctg aggatcctgg atgtattctg caattgccca ggcaatgctc agcccgtcat
    45541 aggtgctcgt gccgcgtccg atttcatcga ggaggatcaa gctgcgaggt gtggcgcagt
    45601 tgaggatatg ggctgattcc gtcatctcaa ccatgaaggt actctggcca cctgcgagat
    45661 tgtcggatgc cccgactctg gtaaatatgc gatcagtcaa cccgatccgg gcttccgagg
    45721 cggggacaaa gcttcccatc tgggccatca aggtaatcag agcgacctgt cggaggtagg
    45781 tgctcttccc agccatgttg gggccagtca gaatgtgcag ccggctggta gccagatcca
    45841 gatgggtgtc gttcgggata aatccgccgg agagatcaag acgttcgacg accgggtgtc
    45901 ggccctgaac aatatggagg ccatcacctt cgtcgacggt tggtcgcaca tagcgattga
    45961 gcgcggcggt ttccgcgagg gaggctacca cgtcaagggt ggccaatcgt cggctgatct
    46021 cttgaaggcg ggctgtttcc tcagcgagtc gtgtccggag ctgttcgaac agcgcttgct
    46081 ccagggccgt cagttttgtg tcggctcccg tgacccgctc ttcaagttcc tttaactcgg
    46141 cggtcatgaa gcgctcggca ttgaccaggg tttgcttgcg gatatagtcg ggcggtacct
    46201 tcccgagatt cgccttcgtg agttcaatat agtacccgaa gacttggtta tatctgattt
    46261 taagcgattc gacgccggtc cgttcgcgtt ctttggcttc gaggccggta atccagcctt
    46321 tgccctcccg acttgccttg cgaagttcat cgacttcagg atggtatccg ctcttcagga
    46381 tgccgccgtc gcgaatcgat acgggcgcct cctgctctat ggctccttcg atcaactcgt
    46441 agagatcctg ggcgttatcc catgacgaca tcaggtcggc gatgagtgga gaatcgagcc
    46501 cggacaattc ggcctgaatt gcgggaagcg aagagacgga aattttcaga gcgaggacat
    46561 cccgcggatt ggcaactccg agggacatgc ggctacagag acgggaaatg tcctggacgg
    46621 ttcgcaatgc cgttcggagg cgaactcgtg cgtcgatctg tcgtttgagt tcgtcaaccg
    46681 ctgtcagtcg ggcctcgatt gccgcactgt tgaccaatgg ccgcaggagc cactcacgga
    46741 gcaaccggct tcccatggcg gtgacggtgc gatccagaac gcccaataac gtataggtgg
    46801 actggttcga cgattgctcc gcacgaccgg caggcctgac gagttccaga ttgcggatgg
    46861 tgacgctgtc caggtgcatg gcgtcatgat cctgtctcac tcgtggcggg cggatatggt
    46921 cgagaggggc cgacggttga gtctccctga cataccgcaa cactgccgcc ccggcttgga
    46981 tgcccagtgt gagtcggtgg cagccgaagg cttccatgga atgcacgcga aactgttctt
    47041 gtaacgtcat gcgacccttc tccaggtcga accacgtcgc aggttgtgcg cagcatcggg
    47101 ggccggtcag atcggcggtc cattgctgtt cgtccggagt tagatcttcg ggaaacagca
    47161 attctttcgg ttcaagccgc gccagttcgt cccgcaggga ggcttctgcc tggatcccat
    47221 gaaactcact gagccaatag tctccggtcg agacttcaag cgttgcgagg ccataggagg
    47281 gctctttgcg tccggtccct gcggatcgaa ggcgcacgta taccgcggcc agcacgttgg
    47341 attccgtgga cggaaggaat tcgctatcga tgagtgtgcc gggggtatag agccggacga
    47401 cttccctgcg caccagaccc ttggccagtt tggggtcttc gacttgctcg cacagggcga
    47461 cggttcgtcc cgcacggagg agtttggcga tgtaattagt ggcggcgtga taggggacac
    47521 cgcagagagg gatcggggtt tcgctggatt tatcgcgtga tgtcagcgca atggagagaa
    47581 gtttggacgc ctccaccgcg tcctcctgga acatttcata gaaatcgccg acacggaaaa
    47641 aaaggatggc atcgcgatag gcctgcttga tgtcgcgata ctgccgcatt aatggggtgc
    47701 tgtcggcgtc acccatcgga gggctctccc tcagcgggaa tgtccgaaga cgtccccacg
    47761 cggcgggcag ccttgtcgag ggcttgtcgg aggcgctcca gatccacctc ggcctcctgc
    47821 agcgccttgg ttttgcgccg gagttcgatg cggccgcgta cccaggccgg aaacagcaga
    47881 atcccggaca ctaacagtcc gacgccgaag gccgccagaa tcggtttata gatcaggatt
    47941 gaggcggatc gaagaccgaa aaaataccgc agagtgactt cctgctcctg gttctgcaaa
    48001 aaaaacgaca gcgcgagtag cagcagcgtt cccaccaata tcaatcggat caatgcgagg
    48061 ccctccttgg ccggttcaat tgtacacggg tttgctgagc cgctctagtg gggcgatttg
    48121 cggagagccg ggagcgaagc gcgtccagca gacgccgtgc agcagggcct ctggctgtga
    48181 gaattgcgcg gcgggccgcc ggggggcggt gcgacaagtg gacatcgagc cggtccgacg
    48241 gaagaccgtc gccccaccga tcggcccatt cgatcgcagt gacagtgtgg ccgtctaagt
    48301 agtcgttgag tccggtatct tcgagttggg cggcggtcag gcggtagagg tcggagtgga
    48361 ccaatggaag gcgtccccgg tattcgtgga tgagtgtaaa cgtcgggctg ctcacgtccg
    48421 tcggttcggc gagcagcccg tcggcgatgc ctttgaccag tgaggttttg cctgctccca
    48481 gttcgccgaa cagggcgaga acttcgcctc cctggaggag tgtgcccagg caacgtccca
    48541 ggcggtgcgt gtgtttcgga gatcgaagca caatgtgcca cggtttgccg atagggggag
    48601 accccgcggt tgtttgcctg agaggtgccg cagacgatgg caccggctta ggtgtgtgtg
    48661 gtcggcgcga gggcatgggg gatgtgctga atgagatcac tcgcaatcat gccggcttgg
    48721 ccgagatgct gggcggccaa atctccggcc aaaccatgaa agtatgtcgc cgcgcaggcg
    48781 gcgtcccaag gggccaagcc ttgcgcgagt agcccgccga ccatgcccgt caagacatca
    48841 ccggtaccgg cggtggccat tccgggattg ccggtcgggc agatggctgc caggccatcc
    48901 gggcgcgcaa tcaccgtgcg ggctcctttg aggatgacaa agacgccgcg ttcccgcgca
    48961 aatctggtgg ccgtgccaag acgatcttca ttgaccgatt gcgtggttgc ctcggtttcc
    49021 aaccttgcca tctcgcctgg atgaggagtg ataatcggcg gtcgcttgca ctcggtcagt
    49081 aacgaggatt tccccgccaa tgcattcaac gcatcggcat cgagtacgca gggcttgtcg
    49141 attcgcttca cgagttcctg taccaggtcg acggtctcgg gatgggtggt caggcccggt
    49201 ccgatcgcga cggcatcccg ggcgccggcg aacgcgagga ggcgatccaa accggaacgc
    49261 gcgaaggtcc gagctttggt ttccggcatc ggcagtgtca tcacttcgag gagttttgct
    49321 tccagaatat cattggcgct cgaggggatt gcggccgtca ccaagccggt tccgatgcgc
    49381 agggctgaga gtgctgccat ggccgccgcg cctgtcttgc cgaccgagcc ggcgatgacg
    49441 ccgaggtggc cataggtgcc tttgtgtgcc gaggggcgtc gtggggggag tgccgcgcga
    49501 gcagccgtat cggtcagcag catcaaccga cttccgatgg cttcgacaaa ggcggcggga
    49561 atcccaatat ccacgagtcg aaccgttcca gcgcaatcga tgccggatcc actatagagc
    49621 cccgccttcg gcaatcccaa cgtcaccgtg agatccgcgc gcacggcggc tcccagcacc
    49681 gctcctgtat cggcatgcag gcctgatgga agatcgacgg ccacggtcgg tcggccgacg
    49741 gcggtgatgg cttcaatcgc atcacgatag agcccggtga cgggggtgga gaggccggta
    49801 ccgagaatcg catcgacgag aatgtccccg gtcgccaacc gttgttggat cgtctccacg
    49861 tcggcggggc atgtaacggc cgatttccct gctgatcgga gaaatcgctg gtacatcgtc
    49921 tttgcatcgc gactcagatc ctcgggggac gcgagcagca ggatgtggac ctgggcctgt
    49981 tttcgtcgta acagccgtcc gacgacaaaa ccgtctccac cattgttccc cttgccgcag
    50041 aggatgatga ccgatttccc ggcaagcggt gcatatcggg cctcaaggtg agtgacgacc
    50101 cccgctccgg ctcgttccat cagcgtcagg gacggcacca aggcttccgt aattgtccgc
    50161 tgatccaggg ctcgcatttg ctcggcggtc acgatcggta tcatggtgtt ctgtgactcc
    50221 cggtcggttt ctcgcagcga gggtgacagc ccgtgccgtg ggggtggccg gactaggcga
    50281 ccaattccct catttcgcgc acagcctgga ccaatccaac catgacggat cgggcgatga
    50341 tgctgtgacc gatgttgaat tccacaatct cgggcaactt ggcgagtcgt ttcacatttt
    50401 tataggtcaa gccatgcccg gcgttgacgc cgaggccgag cttgtaggcc agtttggctg
    50461 cctgggtgat ggcctcgaat tcctcgtctt cttccttcga tcgcttggcg ttggagtagc
    50521 ggccggtatg taactcgaca taggctgcac cgattttgtg tgccgccttg atttgatcga
    50581 gactcggctc gacgaacagg cttgtgggaa tgcctccgtc gcgaagcaga tcgacgattt
    50641 tctgaatgcg atcgcggtgg ttagcgacat cgagaccgcc ttcggtggtc agctcctgtc
    50701 ggcgttccgg gacgagtgtc accaggtccg gtttgacgct cagggcgatc ttggccatgg
    50761 cgtcgtcggc ggccatctcc agatccagtt tggttcgcac gatttcccgc aacatggtga
    50821 gatctcgatc ttgaatatgc cggcggtctt cccgaagatg cacaaccagg ccatcggcgc
    50881 cggccaactc cacgagaatg gccgcggtca aggggtcggg atcggcccct cctcgcgcct
    50941 gccggagtgt cgccacatga tcgatattta cacccagtcg agccattacg cctccgcaat
    51001 ttccctgcag atcataggag aaagatttcg gaactggtcg aaaatcatag acaattctct
    51061 ggttgctgag gcattattaa cagaagacgg tacgggggag caaggaggca gatcgtgaga
    51121 tgcctcactg ttttgagtgt gaccacgata gggtgctcgt ccccaagtcc tgacaatcgt
    51181 agcgaaattg actcgttttg aggcctccat taaaatacgt ccctttcaga ggcatggcga
    51241 ctgaacctgt tcggcagagg acccgcctgt aggagtctga caaattcatg gcgatcggcg
    51301 acggctttta ccgcattaaa cgacttcccc cctacgtatt tgcgcaagta caaaccctga
    51361 aacttgaggc gcgtcagcgg ggcgaggata tcatcgactt cggcatgggc aacccggatc
    51421 agcccacgcc tcctcatatc gtcgataaat tgatcgaagc atcgaggaag gccaaaaacc
    51481 atcgttattc agcttcacgc gggatcacga agctgcgcca tgcgattacc ggttggtata
    51541 agcgcaatta cgatgtggat ttggatcctg agaccgaagc gatcgtgacg atcggctcca
    51601 aggaaggcat cgcccacttg gcgctggcta cgatcgggcc aggcgatgtg gtgttgacgc
    51661 ccacgcccac ctatcccatc catatgtaca gttttatcat cgccggcggg gaagtgcgcg
    51721 ggattgagct gcgccaggac agcgactttt tcgatgacct gttgcgcgtc tatcgccaaa
    51781 ccttgccgcg tccgcgtatt ctcgtgatca atttcccgca caacccgacg accgccgtgg
    51841 ttgatcttga gtttttcaag aagatcgtcg cgtttgccaa ggaacacgac gtgatcgtga
    51901 ttcacgacct ggcctatgcc gatatcgcgt tcgacggcta taaggcgccg agctttctgc
    51961 aagtgccgga ggcgaaggat gtgggtgtgg agttttatac gctgtccaag gcctacaaca
    52021 tgcccggctg gagagtcgga ttctgtgtcg gcaaccgtga agtggtcggc gcgctcgcca
    52081 agctgaagag ttatctcgac tacggaattt ttcagccgat tcagattgcc agtaccgtcg
    52141 cgttgaatgg gccacaggac tgcgtcaagg aagtcgtgca gcggtatcaa aaccggcgca
    52201 atgttctcgt gaacgggctg aatcgtatcg gctggccggt ggcgttaccc cgcgcgacca
    52261 tgttcgtgtg ggcccgtatt ccggacccgt tccggcatat ggggtcgctg gagttttcga
    52321 agctgcttct ccgggaagcc aaggtcgccg tatcgccggg aatcggtttc ggagaaggcg
    52381 gagacgaatt cgtgcggttt gcgctggtgg aaaatgagca tcgcacccgg caggctttgc
    52441 gcgggatccg caaagtattg aatttggatg accaggaatc atgaagagtg agatcggggt
    52501 cgggttaatc ggattcggaa cggtcggcac cggtgtggct cgtgtgttga tcgagaatgc
    52561 tgaattgatt cgtcggcggg tgggtgtacc cgtcacgctg gttcgcattg cggacttgga
    52621 catcacgcgc gatcgcggca ttgcgattcc gccgggcgtg ctcacgacgg atatccagca
    52681 agtcctcacg gacccacggg tggatatcgt catcgagttg atgggcgggt acgatctcgc
    52741 caaacgcgtg attttggatg ctgtgcagcg tggcaaacac gtcgtgacgg ccaataaagc
    52801 tttgctggcg gtgcatggtg aggagatttt tgccgcagcc tcccgtcaag gcatcgacct
    52861 cgggttcgag gcgagtgtgg ggggcggaat tccggttatc cgcgcgttga tggaaggatt
    52921 ggccgcgaac aatatccagt ccatctatgg catcatcaat ggcacctcga attatattct
    52981 tagccgaatg accagtgaag gccagcgttt cgacgtggtg ctggaagagg cgaaacgcgc
    53041 cggttatgcc gaggctgacc ccaccttcga tgtggcggga attgattcgg ctcacaagct
    53101 gaccatcatg gtcagtttgg cctacgggac acccgtcaat ttcaaagaga tctataccga
    53161 aggtatcacc gggcttacgc cgctcgatat tgcttatgcg aaggagttcg ggtttacaat
    53221 caagctgctg gcgatcgcaa aatattccga tggcgaaatc gaggcgcgcg tgcatccgac
    53281 aatggtgccg tcggcctctc aaatcgcgaa ggtcgacggt gtctacaatg cgattcagct
    53341 agtgggcgat gctgtcgaag atgtcgtgct gtatggccgg ggcgccgggt cgatgccgac
    53401 gggaagtgcg gtcgtgagtg atgtcatggc catcgcgcgc aatctgctca aggatgccgc
    53461 cggtcgtgtc ccgcctgcat cctaccagcc cgatcagcgg cggccgttgc gcatgcgacc
    53521 gatggaagaa attacctcgc tctactatat tcgctttatg gtgctcgacc ggccgggcgt
    53581 gttgtcgcag attgcggggg tgctgggtcg ttatgggatc agcatttcgt cggtgctgca
    53641 gcagggacgc aaagaggggc agacggtgcc ggtggtgatc atgacgcata tggcaaaaga
    53701 gcgggatatt cagaacgcgc tccgtgaaat caatccgatg tcctacatct ccgagcccac
    53761 gacgctgatc agagtcgaag ggcgtgatga atgaatgtgt ctctccggtt gtgctgagag
    53821 gatggtcgaa tagattgtta tgaatcgttg gcgtgggatc atcgaagaat atcggaagtt
    53881 cctgccggtc acggcgcaga cccctgtgat cacactgggc gagggcaata cgccattgat
    53941 ccgcgcagcg cggctcgcga agaaaatcgc tccggggatc gatctgtatc tgaagtatga
    54001 aggcatgaat cccaccggat ccttcaaaga tcgcggcatg accatggcga tctccaaggc
    54061 tgtcgagggg ggcgcgcggg cggtcatttg cgcctccacc ggaaacacgt ctgcctccgc
    54121 tgcggcgtac ggggccaggg cgggcattgc cgtgtatgtg ctcattccgg ccggcaagat
    54181 tgcgctgggg aagttgtcgc aggcgatgat gcatcaggcc accgtcattc aggtcgaagg
    54241 aaattttgac caggcgctga ccatcgtcaa agagctgtcg gccacgtacc ccgttgagtt
    54301 ggtcaattct ctgaacccct tccggatcga ggggcagaaa acggcggcga tggagatttg
    54361 tgacgatctc ggtgatgcac cggctatgca tgtacttcct gtagggaatg caggtaatat
    54421 caccgcctat tggaacggct atcgggagta tcgtgcggcc aatcagacga cgcggttgcc
    54481 tcggatgatg ggatttcagg ccgctggtgc ggcgccgatt gtcttgggcc atatcgtcga
    54541 agagccacag acggtggcca cggcgatccg aatcggcaac ccggccagtt ggcaatcggc
    54601 tctcactgcg gtgaaggagt cgtccggtgc cattgatatg gtcactgacg aagaaattct
    54661 gcatgcctat gcgttggtgg ccagtgagga aggtgtcttt tgcgaaccag cctccgctac
    54721 ctccgtggcc ggtgtcatta aattgagtca ggccggacaa ttaaaagaag gtgccacggt
    54781 tgtgtgtacg ttgaccgggc atggtttgaa ggatgccgat acggcgatcg gcatttcacg
    54841 tcagcctcaa acggtgaaag ctacccgcga tgatgtggcc cgcctccttc atgtctgatt
    54901 acctcaggaa cattgcatga aatatttgat cctgcatgcc gacgggatgg cagatctcgc
    54961 gtgtccggaa ctcggtggaa aaaccccgct tcaagcggcc gcaactccga accttgacca
    55021 gatcgcccag cgtggcgagg tggggttgtt gagccttccc tcggaagccg gagccgccgg
    55081 aagtgatgtc accagtgtgg ctgtgctggg atatgatccg aaaaagtatt atccgggccc
    55141 cggtccgctg gaggcggccg gtctcggcgt cacggtgggt gagcaggacg tggtgttccg
    55201 atgcaccatg gtgacggtgc ggggggagtc tgcctccggt agcaaagatc gggcaaccga
    55261 catcaaaaaa ctcgggccgc atgtcctcat ggaggacgcg accgccggtc tcatcgacac
    55321 ggagcaggct cgtgagttgc tcgaagcggt gaatgagcag cttggctcgg aatcgattca
    55381 gttctacccc ggttccgggc atcgacacct catggtctgg gtcggaggaa aatcccgcgc
    55441 gacgtgtctc gatccacaac cgttggtcgg ccggtcgatt gccgatgcgt tgccgagcgg
    55501 cgatggcgcc gacgtgctgc gcaagatcat ggatgcggcg tttttcatcc tgcgcgacca
    55561 tccggtgaat gaagaacgtg agcaggaggg gttgaagccc gccaattgcc tgtggttgtg
    55621 gggccagggc cgtgccgccg tgtggccgcc cttgccggag cgataccaga ttgccggtgt
    55681 ggtggtgtca tccagtgatg tccatcgtgg agttggggtg tgcgccgggt tggatgcggt
    55741 cgagcttccg cttgccgacg gaagcgacgc gaatgagttc acgggttgcg tgcaggcggc
    55801 ggtgcaggag ttggccaaga aggacttcgt ctatctgcat gccgggcttt ccgacgacgt
    55861 gctgcacgcg actgatgtgc aggacaaggt gcgggccatt gagcggttcg atgcccaggt
    55921 cgtcgggccg attctcgcgg cactggcgac gcatccagcc tatcggttgc tggtcgtgtg
    55981 tgatcccggc ctcgcaaaga gccacggtgc tgaggttccg ccgatcctgt atgcgctgtg
    56041 tgatagtgcc gccaagccgt cggccggagt caccaagcgg tttcacgagc aggatgcgaa
    56101 catcgccggg accgcgcctc gcgatgcgac gaagttgatg ctgcgattat tcccgcgcgg
    56161 agtatagagc tgtggcgctg atcgttcaaa aatacggggg aacatccgtc ggcacggtcg
    56221 aacggattca tcgcgtcgcc gaacgtgtcg aacgcgctca gaaagacggc caccaggtgg
    56281 tagtggtgtt gtcggccatg agcggggaaa ctgatcgact gctgaagttg gcgcatgagg
    56341 tcaccggtgc accggacgag cgggaattgg acatgctgct ctcgacaggg gagcgcgtga
    56401 ctattgcgtt gctcgccatg gagctccggg ggcgaggggt gaatgcccgg tccttcacgg
    56461 gccggcaggt cggcattcac accgacagcg cgcacactaa agctagaatt tcccgcgtca
    56521 cggctgatcg aatcaaggaa gcgttgtctg cgggcgtgat ccccgttgtg gcgggattcc
    56581 agggcattaa cgccagctcg gatgtcacga cgctgggccg gggaggttcc gatctgacag
    56641 ccgttgcgct ggcggcggcg ctcaaggccg accgctgcat tatttatacg gatgtggacg
    56701 gtgtctacac tgccgacccg aacatcgtgc cggctgcgcg gcgtctggat aagatctcct
    56761 acgaagagat gttggaaatg gcaagcctcg gcgccaaggt gctgcagagt cgctcggtgg
    56821 agtttgcggc caagtactcc gtgccggtgg aggtcaattc aagtttcaaa gaaggaaagg
    56881 gaacgctcgt gacacgtgaa gatgccgata tggaaggggt catggtgtcg ggtgtgacgg
    56941 gggaccgcaa tcaggccaag attacgattg tcggtgtgcc ggatcggcca gggatcgccg
    57001 cgcgcgtatt cggggccgtg gccaatgcca acattgtggt ggatatgatc atccaaaacg
    57061 tcagccaggc ctccaccacc gacatttcgt tcacggtccc caaggctgat ttgcgtaatg
    57121 ccgtcgatct cgttcagcgt ctatccgaag agatcggcgc ccggtcggtg gctgtgacgg
    57181 aatccatcgc caaggtgtcg ctcatcgggg tgggtatgcg gtcgcattcc ggagtggcgg
    57241 caaagatgtt cgaggtgctc tcgcgcgaag gcgtgaacat catgatgatc agcacgtcgg
    57301 aaattaagat ttcctgtgtc atcgaggaaa agtatttgga attggccatg cgtacgcttc
    57361 acacggcgtt cggcctggat cgggtttcgg cgcctgccct gggctagtct gctcggaggg
    57421 cggtcacggt cggtgtgccg tctcctcgac aggtccgtca tgaccctgct actctcacat
    57481 ctatgaaacg tcgcaacaca gcgccgcggc gccctcgcgg ttcggaacct gtttcggtgc
    57541 cgtcgctcac ggcggcgcag gcgtccgcgc tcgaaatcta cgataccacc ttgcgggatg
    57601 gggctcaggc ggaggatgtc agtttctccg tggaggataa actccgggtg gcgcaacggt
    57661 tggatgaact cggtgtgcat ttcattgaag gtgggtggcc tggcgcgaat ccgaaagaca
    57721 tcgagttctt tcgcacggtc aagaccattc cctttaagaa tgcgaccatc gtggcgttcg
    57781 ggtcgacgcg caaagccagc aactcggttc gaaaagataa aaatcttcaa gaactgctcg
    57841 cgtcaggaac tccgaccatt acgctcttcg gcaagagttg gtcgtttcaa gtgacggatg
    57901 cactgggcat tgcgctcgcc aagaatttgg agttgatcga agactcgatt cactacctgc
    57961 ggtccaagga tcgccgggtg ttttacgatg ccgagcattt tttcgacggg tataaggcca
    58021 atcccgacta tgcgttgcag acgattcgca gagcgattgc cggaggagcc gagcgggtca
    58081 ttctctgtga taccaacggc gggaccatgc cgtgggaaat ccgtgaaatt tgccgcattg
    58141 tgaagcagga atgtcccgtg ccattgggga ttcatgcgca caacgacagc gaaatggcgg
    58201 tggcgaactc gctcgtcgcc gtggagtcgg gcattctgca ggtgcagggg acgatcaacg
    58261 gcatcggtga gcgatgcggc aatgccaatc tctgctccat catcccgaat ctacagttga
    58321 aaatgcagcg gcctgcgctc ggcaataagc tgacgcacct gaaggatgta tccgggttcg
    58381 tgacggagat cgccaacctg atgcccaata agcggcaacc gtatgtcggt gacgcggcgt
    58441 ttgcgcacaa gggcggcgtg catattcatg cggtgctcaa gaatgcggcg acctacgaac
    58501 acatcgatcc cgccgcggtc ggcaaccgtc gtcggatctt ggtgtcggat tatgcggggc
    58561 gcagcgggct cctggaaaag gttgaagcgt acggcatttc actgaacaag aatcacgcaa
    58621 agttgcagga actggtcgag acactcaagg agcgtgaaag ccaagggtat caattcgaag
    58681 gcgcggaagg atcgtttgag ttgctgatgc gcaaagcgat gggcagccat cgcccgtctt
    58741 ttcagctgct tggtttccgg gtgatcgtcg agaagaagca ggagcacagt ctgctgctgt
    58801 cggaagccac cgtgatggtg aaggtcgggg aggtcgtcga gcacacggcc gccgtcgggg
    58861 ccggtcctgt gaatgccctc gaccatgcgc tccgcaaagc cttggaaaaa ttctatccgc
    58921 aacttcggga agtgaagctc ctcgactata aggtccgtgt gctctctgcg gataaaggga
    58981 cggaatcaaa ggtgcgcgtc ctgatcgagt cgggcgacca taaggacaag tggggcaccg
    59041 tcggtgtgtc ggaaaacatt atggaagcga gttggcaggc gttggcggac agcattgagt
    59101 ataaattgct gctggcggat cgctaggctc ttttcacctc cgtcgggcgc acataatcct
    59161 tgacagggca ggtaacctca cgtaacttat ggggaaattc tttgtcttgg acgctcgaca
    59221 ccaagaagca cttcctaagg aacgactcta gtggatggcg gggggaaagg catgagaaag
    59281 gcggatatcg cgaacgaaat cttcaagcag gttggagtct ccaagaacga tgcagccgat
    59341 atcgtcgaac tcgtgctcaa tctgctgaag ggagtgctgc aaaaaggtga cgcggtcaaa
    59401 attgcaggat tcgggaattt tgtagtacga agcaaagggg ctcgcaaggg acggaacccg
    59461 cgaaccggag aagaaatcgg gattactccc cgtcgagtgg tcacgtttcg gcccagtcag
    59521 gtgtttaaaa agtacgtcaa ttcctagtcg ctaccttgtt acgagcacaa gcagcatgag
    59581 gaccggtcat ggggaatgag ccccgactgg gaagtaaagt tttttacaaa atcggcgaag
    59641 tcagcaagat ttccaaactg ccggcttatg tcttgcggtt ttgggaatcg gagtttagct
    59701 tcctgagacc gaagaagagc cgtgggaatc agcggctcta tgtgcagaaa gatgtagata
    59761 cggtactgga gatcaagcgg atgctctatg acgaggggca tacgctggcc ggggttaagc
    59821 gctactgggc tcgtcgagga cgagttaccg tgaagaagct gagctcgcgg aaagaactgg
    59881 cacaacgtgt caggggcgat ctccaggcga tcatcagaat gatcgattcc cattcgtcct
    59941 gaactgttct tgtgatgcgg gcggctgcgt ccaattgtgg gtacatcgat gtcgtgtgtg
    60001 tcggggcgta gcgcagcccg gtagcgcact cgcttggggt gcgaggggtc gtgggttcaa
    60061 atcccgccgc cccgaccatc ggcacgatgt tctctaccac tgtcagtgtc ctgagggacg
    60121 ttgatcatta cggacgatct tgtgagcttg ttcgagtgcg ttcctgcctc tttcgtcccc
    60181 actcttgatc cagcggtttc ggtgctcccc tttcacgacg tgtgcgaagc ataaaggagc
    60241 ggttgtggcg cgtgtacgga tccttgtgac caatgacgat ggaattacgt ctcccgggat
    60301 tcacgcggtc gcggcggcgt tgggtgcatt gggggaggtg tggattgtcg cacccgaccg
    60361 ggagcgcacg gcagtcgggc atgcggtcac gttacataag ccccttcgtc ttaccaagat
    60421 ggcaccccgt gtatttatgg tgaacgggac gcccgtagac tgtgtcaact tggctctggt
    60481 gaaggtattg ccagggcggc cgtcgttgat tgtgtcgggg attaatcgag gggtcaatct
    60541 cggcgacgat gtgatgtatt ccggcaccgt gtcgggggct ctggaaggaa cgatcctagg
    60601 cattccttcg atagccgtgt ctcaggaagg caacgagaca tttcgattcg atgtcggagc
    60661 gcagtatgcg gctcgagtgg ctgctgaggt attgcggcat ggattgccgc cggaaacgat
    60721 cctgaacgtg aatattccga atgttccggt ccggtcgatc aagggtgtga aagtgacctg
    60781 tttgagtcgg cgtcgtttca acaaccccat cgttgaaaaa gtcgatccga ggggtcggaa
    60841 gtattactgg atcgccggga cgcgtcagtc gtggagtcgc gagaacgatg ccgaccacga
    60901 agcgctggag cagcgcatgg tgtcggtgac cccgattcat ctcgacacga cccatcatga
    60961 ggtgctggaa cagttcaagg cgtgggagca accgctctcg cgaccatccg cgcgtcccaa
    61021 aacggtgaag ccgcatactc gaaggagagc ccaggcgtgg ccggattgat cgagtggctg
    61081 atagaagtgc tcggccggtt cgtgatcgcc acgatttcga cgttcggcta tacgggtatt
    61141 gtcattacga tggcgatcga aagcgcctgt atcccgctgc cgagtgaaat catcatgccg
    61201 ttttccggct acctggtctc caccggccaa ttctcgatga tgggcgtgac ccttgccggc
    61261 gccatcggga acgtgctggg gtcgttggtg gcctactacg tgggtgtgtg gggagggcga
    61321 ccgtttgtcg agcgctatgg ccgatatgtt ctggtgtcgc agcatgatat ggaactggcc
    61381 gatcggtggt ttgcgaaaca tggagaggcg gcggtgttga tcggccgtct actgcccgta
    61441 gttcgaacgt tcatctctct cccggcgggg attgcgcgca tggacgtcaa gaagttcgtg
    61501 ctctattcat ttgtcggagc gcttcccttc tgttatgcgt tggcctatgt gggcttgaag
    61561 atgggggagc actggaatca attacgtcag tatttccatc acgcggatct ggtgatcggg
    61621 ttgtgtcttg ccgtgggcct cgggtatttt ctctggtccc actggccgaa gcgtcgtccg
    61681 tctatggagt aattgcgcgt tatgttgcgt gtccacaata ccctgaccgg aagcaaagag
    61741 ccgttcgaac ccttggtgcc cggcaaggtc cgcatgtatg tgtgcggggt gacggtgtac
    61801 gactattgtc acatcggaca tgcgcggagt gcgctggtgt tcgacgtgtt gcgccggtat
    61861 ctggaatatt caggtttcgt cgtcgagttt gccaagaact tcacggatgt cgatgacaag
    61921 atcatcaagc gggcgaatga acagggcgtg agctgcgagc acgtcaccac cacctacatc
    61981 aacgcctatt acgaggatat ggagaagctc ggtgtgcgcc gcgcgacgct cgaacccaga
    62041 gcgacggaac atatcgccga cattgtgagg cttgtcgaca cgttgctcgc caagggaatg
    62101 gcgtatcgtg tcgacgggga tgtctatttc caggtcgatc ggtatccggt gtacggccgg
    62161 ctctcgaagc ggaacgtcga tgatttgcag gcaggagcac gggtggatgt cgatgagcgg
    62221 aaacggcacc cgatggattt cgcgctgtgg aaaggcagca agcccggtga gccttcctgg
    62281 gatagcccct ggggcccggg tcgtcccggc tggcatatcg aatgttccgc tatggccatg
    62341 cggcatcttg gtgaaacctt cgatatccat ggcggcggga tggatctcat tttcccgcac
    62401 cacgaaaatg agatcgcgca atcctgcggg gccacgggca aggagttcgc gcggtactgg
    62461 gtgcacaacg ggttcgtgca gatcaatcaa gagaagatgt ccaagtcgct gggcaacttt
    62521 tttactattc gcgaaatctt tcagaagtcg gaatggtccg acacggtgac cggcgagatg
    62581 ctgcgctatt tcctcctttc tacgcactat cgaagcccct tggatttttc cgatcagagt
    62641 ctgaacgaag cgaaaaacgc actcaacgga ttttacgatc tctttgaacg gctgaatgaa
    62701 tcggcctcag cccatggtgc ggccgatcag cagatgcagg agtccacgat gcgggcgcga
    62761 gcggcattcg tggccgccat ggacgatgat ctgaatacgc ccaatgcgct ggccgcctta
    62821 cagacgttac gtggggaagc gaacaaggct ctcgaggtcg gattgtccgg tgagatgcgc
    62881 cgggtggtgc ggcaagagtt ccgcgcctta gggatcgtcc tgggactcct ccagaccgac
    62941 acctggcgat ttaaaagtca gcggcagcag ccatcatccg gcggagcgga ggcatccacg
    63001 gacaccctgt cagacgagga cattgccgac aagctagccg cacgtctcgc cgcaaagcga
    63061 tcgaagaatt accaactcgc cgaccagtta cgagcagagt tggcctccca tggcattacg
    63121 attgaggacc ggccggatgg caccagtcga tggaaacgat aactcgctgg aggtgatcta
    63181 cggcctccac gccgtgcgcg aagcgcttcg cgccggggtt cgtccgatcc aacgtctcct
    63241 cgtgttggga accgaccgac agttcgggga aatcgcgcgt ctggccaggg atcagcgtgt
    63301 gcccgtgcga gtggaaccgc gggctgcatt ggatcgcctg gttccttccg gtcaccacca
    63361 gggggtggtg ggtgtggtcg ctgccaaagc ctatgcggag ccggacgata ttctcgcttc
    63421 cgcacaggct gaagggcaga cgccactgct ggtcctgttg gacggtgtgg aagacccgca
    63481 caacctcggg gcgattctgc gaacggccga ggcgtcgggt gtgcaaggcg tctttattcc
    63541 ggagcgccgc gcagtggggc tgacgagtgt cgtggcaaag gcctctgccg gggctgtcga
    63601 ttacatgccc gtcgggcgcg tgccgaatct gtcgcgcttg atcgaacgtc ttcaaggcgc
    63661 aggcatctgg gtctatgcgt tggatgctga ggctccgaag ccctataccg cgctggattt
    63721 cagcggcccg gtagcgctcg tcttcggcgg ggaagggacg ggggttcgcc caggggtgcg
    63781 tgacgcctgc gatgaggccg cccacattcc catgatgggg aaagtcagct cgctcaatgt
    63841 ctcggcatca gcagccatcg tgctgtatga ggcattgcgt caacgtcggg aaggtgcggg
    63901 aaagaagccg taaggaagga tgcgaagggc tcaccggagc ttcagcattt tcccttggat
    63961 ggcggccttg agcagttggg cggtattgga gacccggatc tttttcatca tattcgcccg
    64021 atgcgcctcg actgtcttga cgctgatctt taaccgctga gcaatttctt tgttcttgaa
    64081 tccggtccag atcaactcaa ggatttcctg ctcgcgggtt gtgagggctt cgggacgtcg
    64141 tttgcgaggg ggaaggaccg gatccggtgc ggatactttg gctcgacttt tcgtggtctt
    64201 ggcgactggc gacatgtaca tctagctcca cttcaggtaa aggcaattca ctgattgagg
    64261 tttgcgatct tccgcgccaa aattgctccc ggattatacg cagtgccccc ggcgtttgta
    64321 aatggaaaga gccaagacga ggggtcctcg cgcctgctct tattgcccta ggattgaata
    64381 cgtatactta ggagggggtc atgtcgagcc tggccacatt gtttcaggaa tgttcatgat
    64441 ttcatacgtt acggtactgc tattcggcgc ggtgattggc agttttttga atgtctgtat
    64501 ctaccgcctg ccgcgtgaag agtcggttgc ctggccggcc tctcactgtc cctcctgccg
    64561 ccaggccatt gctttctacg acaacattcc gattgtgagt tatctgcttc tgcggggccg
    64621 ctgccgggcc tgccatgcgc caattgcgat tcagtaccca gtggtggaag cggccaacgc
    64681 gatcggatac gtgctggtct tttggacgtt tgggtttacg ccggcggcct gggcctatgc
    64741 ggcgctggcg tctgcgctta tcgtggtgac ggggaccgac ctttcccata cgatgatccc
    64801 ggacgccatt acgctgccgg gaatcgtcat cggtctgctg tgtgccatct tgatcctccc
    64861 gatcggactt gtcgattcct tgctcggtgt gttgttgggc ggcgggatct tatggttttt
    64921 agcttggatc agtccctatg ttttcgggaa ggaagggatg gggggcgggg atatcaagct
    64981 gatggccatg gtcggggcct tcctcggctg gcagccggtg ttgttggcga ttatgatcgg
    65041 atcttttctg gggtccattg tcggtgtcgg gctcatcgcc atcggggtca tgcggcgtga
    65101 gcagtacatt ccattcggac cgttcttggc agtgggatca atccttgccc tgttgtttca
    65161 tcagcccctg cttgagtggt attgggcgct gatcgacatt cctcagtaac tgccttcaaa
    65221 tcgttatcca gagccaattt ccaaggatca gcgcctcgcc gaactgtatc tgaacggatg
    65281 actgactgta tccgattcgg aacagttcat aattgttcac gcgctttttt ctgtcctacc
    65341 tcaggcaaca ttccacggct cacggcagat ttcctcaaca tttctatagc ttcgccggcc
    65401 gcttcgtaga agaggcggcc tcattgcttc tacaggcgtc gggaatgaga aacggcatgg
    65461 aacttggaaa tgctctacgc gctcgaagaa aggagccggt atgaacgaac gtggcgggag
    65521 tcttgtggag ctgtgtacgg tggtcgcgat catcgggatt gtggtgggcg tgagtgtgcc
    65581 tggatgggcc gccctggtcg caaaacatca acagcgcgcg gtgatggtgg agatcgcatc
    65641 ggagttgcgc atggcccggc aattggcgat ggcgaggcat gagcgtattc gagtggtcgt
    65701 ggatgcggaa cagtctgaat tgcgcacgga atgcatggac tgtgacacaa gcttgctgcg
    65761 ccggtatgag ttcgctcaca aaggcacggc ggttgagtcg atgacgacga aaccggaaat
    65821 catgtttcag cccagcggtc gttcggccac ggcgaccacc atggtcctgg tcgacggtcg
    65881 tcgtgcgaca catcaagtca cggtgagcat caccggacgg gtgaccctgt catgacccaa
    65941 tgtgagccga atcttgtgac gcgacgatct gcgttgatga gtgccaaagg gttcaccctc
    66001 gtggagagca tggtggcgtt ggtggtgctg tcgatcggcg tgatcggaac gatcggcatg
    66061 tgcgaatggg ctgagcgcgg tctgcagcgc ggggcgttga cgacaaccgc gctggcgctc
    66121 gctgaatcac gcctggaggc gaagcggagt gtggcgtggg agcggttgtt gatggacgac
    66181 gtggaccaag acggaatagt ggagtccgtc atgcacgacg atggcttgca ggatgatcag
    66241 acgaacggcg acggcatctt cacggccagt gccacccagt ccaacatccg gcttgtctgg
    66301 accgtggaac tgaatcgggg ccacgagccg gccggggcga gcctggcgac tatcgaggcg
    66361 cgtgcaatct tccggactat gggagggcag gagaaagagg ttcgcgttcg cacaatcagg
    66421 gccaatcctc ggtatgtcgg cctgtctgtg ttgtcatgag gcgcggcatg gtcaacggtt
    66481 cgaacgcgcc gaagcgcatg aatattgggt ggtgcctcgg ctctgccgga gcgagtctca
    66541 tagaactcct catcgcaatg gcgatcagca gtatcgcaat atccgcttcg attcatgtct
    66601 tttcctcagt cggcttgcgg ttcagcggac agcattcgac gatggtgacg aatcaggacc
    66661 tgcgcctcgg actggatgtg ctgtgcagcg aagtacgtct ggctggagcc gggttgctag
    66721 gtgaagattc gccgtttctc aaggcgaagt cggacgagat cgaattcttc gccaacttga
    66781 gtggtgcgtc gactacgctg acacaagtcg cagaaatcgg ccgccaggat ctccccgtcg
    66841 aagacggagc cggttggccg aaaggcaagc aactactggt gtgcacagcc gtgcattgcg
    66901 cctggagtca gttggctgcc gatggacgga agcagacgct gacattggtg acaccgactg
    66961 ccgaacagtt tcctgtgcat agcgccatat tcctgctgaa tcgagtgcgt tactacgtga
    67021 agcgccagga tgatggggcg atgcgggtca tgcgtgatgt ggatggcggt gcgagtacgc
    67081 tcttgggcga tgtgagtcag ttcgagctgc aatattttga tagggagggg cgtgtgacga
    67141 cggacttgag cgaggtggtg cgggtacgtg tggcgataca ggccggacga caaggagcga
    67201 ggctggtgcg ggacgtggca atccggatgt aataggtaaa aggaataact atgaagacaa
    67261 gcatgggacg atcacgaatt gcggtgcggc gagatgagag gggaatggcg ttgttggcgg
    67321 tgctcatggt ggtgtttctg ctgaccttgc tggggatgac ttctatgcag ttggccggcc
    67381 aggagatcgt cggcgccagt gcgttacaag aggaacggct tgcgcaccac gccgctgaag
    67441 cggcggttga tgtggtgatg ggatggtttc acgatcctgc gctgcatccg cagggagtcg
    67501 agacgacgtg gttgacgaaa cggctgctga atgcacaggg agatgcctcc tatttcgatg
    67561 cacaggggcg cgcacagttt gcgggaaccg gtgcgtctcc ggatgtggtc ttcgatgcgg
    67621 cccatgctca gcacgaccgg cttatgaacg atcctcaaac gggttggttc aaatccctca
    67681 aggggcttgc gaggattctc aagttacgag tctatggggc gacgcggcca ggactgctct
    67741 gcacggtgga ggtgacggcc ggagccggcc atacctcacg ggtaacgaaa acggtatcag
    67801 tcgaattcgg cgcctatgct gttccggcgc tgcacgcgcc gatccagggc ggcacagtgg
    67861 gaaatgagtc cgcgccatcc tcagccggtt ccgtgttcgc ccattggggc gacatggtgg
    67921 tacgtggcaa ggcgtatttc cctcggccgg aggcggtccc gatgaagagc gggttggcgc
    67981 cggtgacggg gcagtcctat gacgaaatga cgcaccgcga ggatcgctgg ttcaccatcc
    68041 gactcggtgg cgacgccttt tttgcgcagc ttccggcaga ggcctggtcg ggagttccac
    68101 tgaatcttca cacgcaccag gatccggtgc ccggtctcaa ggtagaccag tgggattacg
    68161 acactttgaa gcgaatggcc aggcagttcg ggcagtacta cggcatagac cgggatggat
    68221 tgctctatgc cggcggtgtg attcagccag gagcggggca cccagcctcg gaagtgtttg
    68281 cctcgaaggg ccccggtgac catcgtggat tgatcttcgt cgacacgctt gatgggatgc
    68341 ctccacgacc ggataatttg gggacgatcg tgcttgatca agagtatgcc gagggcatct
    68401 tcattgtgaa tgcgcatgtg ctctggaagg caggggcgca tgggaaaccg gtttcagctc
    68461 tcagtccacc gccggaaggt cagcaatctc ttggagccag gattcctgtt caactgtccg
    68521 gtattcatct gcagggtgtt ctgtatgtcg cgggtgacgt gcggtatgca gggcatctca
    68581 aagtctacgg tggggtggtg gcgcaaggag cgattgtgga cggcacgaac gggtcgggga
    68641 tgttggaagc ttggtgcaac cacgacctcc gcgatgggct ggtgcagggc atgccgctgg
    68701 tgtttgtggc ccctggaagc tggcaggcca aaatttgacg gcagcactac gaggttgacg
    68761 cggtcactct gccagcatgg tgagttcaga caatcatcta caatcgaatc gtctcca
//
LOCUS       DAORRQ010000019        64915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_23_length_64915_cov_61.631021, whole genome shotgun sequence.
ACCESSION   DAORRQ010000019 DAORRQ010000000
VERSION     DAORRQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 64915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64915
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23_length_64915_cov_61.631021"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1437
                     /locus_tag="PK224_08070"
     CDS             199..1437
                     /locus_tag="PK224_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB15731.1"
                     /translation="MKSRLTAALIGVALTLTGLLVLEGVSRALLTAQADLAPEQPDWY
                     RYTPDLGWERRPHFKGLVAGEMHRHEPARYLREFDAQGLFAVDTAQIGDTTHKRILAI
                     GDSNTFGWGVPTRNAYPEVLDDLRPDADVINLGVSGYSSFQGYTALLKYFDRVRPDVV
                     IASFSFNDRRVVPSEAATDSHEKFEREALLHRMDFREKIYLYRGLQHLLIRLHLMKRS
                     GDGSTTVDARTAPTRVSPDQYRQNLERIARFCQERHVPLVFVAFQDNPAHSEHLRAGI
                     AHLNAGRQAQAEAELRIAVNMDNWFSDLARKYLAVVLEQRGASEEATITASLTLPAWK
                     LTHGGRPLRLDSEYNDIVRAVARRHGAVVVEAGQVLAQDASLYLDLAHPDERGHRIVA
                     TLVNNALDSLLHPPQVAAQP"
     gene            complement(1702..2103)
                     /locus_tag="PK224_08075"
     CDS             complement(1702..2103)
                     /locus_tag="PK224_08075"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HRB15732.1"
                     /translation="MEIRIYYEDTDCGGVVYYANYLKYFERARTRYLEERGLSVAELR
                     DGGTQFMVVHAELDYRSPARYGDTLTIDTSLAAVGQASLTFAHVMRERTSGRVVVEGS
                     AKLVTVDDNLKVKRLDKPTLAALQGPPDTRS"
     gene            complement(2146..3402)
                     /locus_tag="PK224_08080"
     CDS             complement(2146..3402)
                     /locus_tag="PK224_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HRB15733.1"
                     /translation="MPADTPVKALILAFTDAPSLAAYVINRLQPELLCFFVPESAKTL
                     VEEAVQPLVQQMPKRWDWVVTPDPADVIACHQALSRSIHDLFRNWAVQVGEVVVDLTG
                     ATPAMAAALAMVSQPWTSRVISLVDAEGREEGEAIAIGGLAKYWLQGNPWDESAVVVR
                     REASDAFNHGSFRAAATLFHTLEARVSGGQKPLYRALGELASGYGLWEQFHYRQAWDK
                     LKTSLKALDMASLWGGPPGLKALLPLIKANSGFLEKLVLDPADVKEGVVLDLLAHAHR
                     RAHVDHDYERAMVALVRAMEACAQRQLYKQYKIKSWDVQPEQLPESMQDTCHTCYLDD
                     VDGKYKLPLQSQFRALAGLGDQMGQAFLRDWPKMKPLLDAANHAVLGHGFEAIKSERV
                     QQLSDVVMKLTGVNESSLPKFPVLNL"
     gene            3545..3979
                     /locus_tag="PK224_08085"
     CDS             3545..3979
                     /locus_tag="PK224_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRB15734.1"
                     /translation="MTWWLWAFLGLFLLGSEIVTPGGFYMLFFGIGALVVGALVGLGM
                     IHSEWMSWLLFSVFSVASLVILRPPLRRLMTADRGNVSSVDTMGGETAIVLDDLPPGA
                     TGKAECRGSTWNAHNAGDKPLLKGQRSLVDRVDGITLWIKPE"
     gene            4007..4945
                     /locus_tag="PK224_08090"
     CDS             4007..4945
                     /locus_tag="PK224_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRB15735.1"
                     /translation="MPGGLWVVIFLAGLVLLVISKTARVVPQQSAYVVERLGRYSRTL
                     GAGFHILWPFLDSVQYKHSLKETAIDIPEQICITRDNVQVGVDGILYSKVLDPQRASY
                     GISDYRFAITQLAQTALRSEIGKIELDRTFEERTNINSQVVNELDKATEPWGVKVLRY
                     EIKNITPPKDVLAAMEKQMRAEREKRAVILTSEGERDAAINQAEGEKQQVIKASEAKK
                     QQQINEAEGAASAIMAIAGATADGLRKVAESTQIPGGYEAVQLRVAEQYITKFGELAK
                     ASNTLVLPANVSDVGSMLTLAMNMITKRSSPTSPGK"
     gene            5000..5578
                     /locus_tag="PK224_08095"
     CDS             5000..5578
                     /locus_tag="PK224_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRB15736.1"
                     /translation="MKEFAIKAFDDSAEIKRRFARDHADRIVQVAQLIGGAFQAGHKV
                     LLFGNGGSSTDAAHIAAEFVGRYKRERAPLPAIALATDIAAITCIANDYGFEELFARQ
                     VQAHGQRGDVAIAISTSGNSPNVLKGVEAAKALGLTTIAWTGGTGGKLAGMVDYAFVV
                     PSTLTARIQESHITLGHVLCELIEDHVLANPA"
     gene            5562..6560
                     /locus_tag="PK224_08100"
     CDS             5562..6560
                     /locus_tag="PK224_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15737.1"
                     /translation="MPTRRKRPTAASTPPAIIRPIDASRLKTYPLQRRHSKVQVSNFA
                     KVWTKGRSFARFLDSLPDILAVKTLREVAQAIAKAHRNGRPVIVGMGAHVVKVGLGPL
                     LVDLMERGIVTALAMNGAVIIHDFELAFMGHTSEEVDAEIDSGRFGMAEETGRMLNEA
                     ITLGMKEGQGLGESLGSYIHRRKQLFPHRATSLLATGFRLGLPVTVHVAVGTDIIHMH
                     PSADGAAIGAGSLLDFRRLAAVVSEMEGGVYLNLGSAVILPEVFLKAVSLGRNLGHPL
                     TNITTVNMDFLTHYRPMTNVVRRPTQKGGKGYALTGHHEIMVPLLAAAVIEELSGR"
     gene            6618..7229
                     /locus_tag="PK224_08105"
     CDS             6618..7229
                     /locus_tag="PK224_08105"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRB15738.1"
                     /translation="MSPSPEPLLTIWSDLNRRYFQGTLPPIPIEWSRRLTSSAGMFVC
                     RIGPRQPLARTTEAPTRRRCIRLSAVLLKPNNPDSSHDTVMTLAHEMIHQWQYDILKR
                     RPNHGADFRRMMGQMNRDGLGITIYHSLGKEVAALAKYAWRCQHCGCLYQRQRRTIQP
                     RRHFCGACRGPLRELPAVVPASTAAPAHTSAAGPQLGFPFTPV"
     gene            7237..8430
                     /locus_tag="PK224_08110"
     CDS             7237..8430
                     /locus_tag="PK224_08110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRB15739.1"
                     /translation="MSGRWPRQILFGDIDAMFASAAVLADPSLAGKPVAVGGPPPRGI
                     VAAASYEVRRFGVRSAMPTIQAMRLCPQLVLVPPDRPLYQRLHRQLQDITDRFFPETE
                     WTSIDEFYADTTRLQTRHPDPRELGQALKIDITRVTGLTCTIALASGKTVAKIAADAH
                     KPDGLAVIEPGSEAAFLAPLPIRSLPGMGPKSTEAIERLGIHTVGDLLEPRFEQSLIR
                     LLGSRLAAFQSLARGHDSDPVVADREAKSMSHETTFDEDTNDPALLEPIIHGFLETLT
                     HDLRLEGLAAGSFTVKLKDSHFRITTRQRKFSSPVNYDPDMWPDIRLALAELLQPRSR
                     YRLVGLGLSDLVPAPEPLFDRRQRDAVAILDRLIEQHGAGVVRLGGLPQGGDSPKKGG
                     RRRED"
     gene            8521..9003
                     /gene="rfaE2"
                     /locus_tag="PK224_08115"
     CDS             8521..9003
                     /gene="rfaE2"
                     /locus_tag="PK224_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRB15740.1"
                     /translation="MHTKVTTRQELASRLEERRRQGQRIVFTNGCFDLMHIGHIRYLQ
                     AARNLGDVLVVGVNSDESVRALNKGTDRPIVPDAQRAEVLAALACVDYVVTFSEPDPG
                     ALIATLQPDILVKGGDWSVDRIVGRETVEARGGRVQTIPLVPGVSTTTLVQRIRSTTA
                     "
     gene            9011..12484
                     /gene="mfd"
                     /locus_tag="PK224_08120"
     CDS             9011..12484
                     /gene="mfd"
                     /locus_tag="PK224_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRB15741.1"
                     /translation="MPPLIPLHVTQWLAPAREALRSGSGKPCLMGLHGSTAGFGLALL
                     THSVPSQPLADRSWLIVAKNDDEAERLYRDTLFFRTLCGQSGDDLALFPKWETLPYES
                     TVPHIDLVARRMQTLNRLCTTARTVLFTSIPALTQRVLPALVFTDAILQFQPNGSLER
                     EVLVSSLLRLGYRKGSVVEIPGEFSIRGGIVDIYSTAYADPLRVEFLGDTIESIRFFD
                     PATQKSTDKIKQAWVLPARELIRPEDAPDALAPLAADAEWHAPSVYGTMDSLLDYFPQ
                     PPVLVLDQPGTLKAHTEECWQAIEEGFLRHEDRTDPNPYPTPDNLYLTWDQVLAATQG
                     YATLALEPVTAPDASWDPVLTCPAQTPASVGLGQRGTAFSHTLEMLDRLREGGPVVLV
                     ARSQGQVGRLLALFGEHDRPAVEWKPSALSAGGAQKAPFSVLNGEVSAGFLSPDLRLV
                     VLTEEELFAKGARHKPQPKSKAATFLSSLEDLNIGDYVVHMQYGIAKYQGLRRLSVQD
                     FDSDFLVLEFAGTDKLYVPLDRLSQVQRYAGADAHVPRLDRLGGTSWAKTTARVKKDI
                     EEMAHELVDLYANRELVHRTSYGKDSMLYHEFEAAFEYEETPDQRKAIEDIANDLAST
                     KPMDRLVCGDVGYGKTEVAMRAAFKAVEENRQVAVLVPTTLLAHQHYDNFAERFAPFP
                     TRVALLSRFQSPKDTKAIVKDTEAGLVDVLIGTHRLLQKDVKFRNLGLVIIDEEQWFG
                     VKHKERLKQLRTQVDVLTLTATPIPRTLQMTMASVRDLSIIDTPPSGRLAIRTQVLRF
                     SEKAIREAILRELGRGGQTYFVHNRVETMERMGSWLQELVPEARIVMAHGQMDSKPLE
                     AVMLKFFHREADILIASAIIQSGIDVPTANTIIVNRADTFGLAQLYQLRGRVGRGGEQ
                     AYAYFLVPDEGNLSEDAQKRLTAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHI
                     AAVGLDLYLQMVEQAVQRLKGQVVEEEPDPTLRLSVSAYIPEDYVADSHQRLSLYKRL
                     SSCGQLGDLALMHGEIEDRYGHPPDPVERLFELMQIRLLAKQLRLSSVVEQPHAVVIT
                     FDPKATVSETAVQALMDRYKKRLRFLSPLSFELQIPHEDWSLVFPELNATLQTLHVCG
                     TNNQGPRTAST"
     gene            12515..13411
                     /locus_tag="PK224_08125"
     CDS             12515..13411
                     /locus_tag="PK224_08125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRB15742.1"
                     /translation="MTPPRTRQISSWQTRLTSAALGLCALGSLAACTEPPQEEPVIAM
                     INGRSITQSEFDIRWEDLSQATRARYEKEGGKRRFLDELIMRELLMQEARKQGLDQSD
                     EIREKTLRYREQLILDELLKDRIKTKVEVSKEELDAYLGKHANQLLANPKVQVSIMLL
                     PNVYAAKDLKRQVEAGGNFTRFALRYSIDERSRAKGGDLGPYKKGLLEPELDALIPSL
                     HPGVISDPIKTAQGYYLVKVSPLEPEILQADQATRERLRQELLAEKRRKRLDDVFAEL
                     RTGATIRLADAARYVTDEPARP"
     gene            13521..14555
                     /locus_tag="PK224_08130"
     CDS             13521..14555
                     /locus_tag="PK224_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRB15743.1"
                     /translation="MNDQSLSAHTCLHGYRFFLSQKGRPAPGPVRSATGFMTTGALFF
                     CLCLLFVLIANAVAAKLEDRIVAVVNSDLIMLSELKRDLLPEQDRLRKLYKGEDLERR
                     LKTAEAMGVTKMIERKLQLQAARNKGVDVSDQEVVQAVEEMKKQGEAIDSADPNTARS
                     IREQLTLMRVVDREVRGLIMVADSEMKRYYQEHQDRFAYPEEYQLSQILIKPRTPDGL
                     SAAHGRAEALLATLKQGEPFEDLALRFSDGADASRGGRLGLVRQGELIPALEQALTSV
                     DVGQITGIVETAEGLHIVRVDDKKPRQFRPFEQVKAEIQSLVFQQKTEDQYQIWMADL
                     KNKAYIEIKF"
     gene            complement(14717..15352)
                     /gene="yihA"
                     /locus_tag="PK224_08135"
     CDS             complement(14717..15352)
                     /gene="yihA"
                     /locus_tag="PK224_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRB15744.1"
                     /translation="MKLLSAEFIKSSVSPEQFPPDRLPEIAFVGRSNVGKSSLINSLL
                     HRKKLAKVSKTPGKTRAVNFFTVRTSDPKLSLLSLVDLPGYGYAKVSKTMRAQWGPMI
                     EQYLEQRQSLGAVILLIEARVWMPQDVMTVQWVQSLGCGMIIVLTKADKLRQSERAPS
                     LVAVRTACGLGPEVPIVLYSAETHEGRDALWGEIRTQFNGLRKHECGTREL"
     gene            15454..16239
                     /gene="mtgA"
                     /locus_tag="PK224_08140"
     CDS             15454..16239
                     /gene="mtgA"
                     /locus_tag="PK224_08140"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRB15745.1"
                     /translation="MSKSTGSLFGRMLLWATILVGLPAAALALYWLVTLPDVSHLAKH
                     HPTETAVMEARRAQAKEQGHPLHIKFSWVPLARIAPALQRAVVAAEDASFFAHEGFDW
                     EGIKDAALYNLEVGEFKRGGSTITQQLAKNLYLSSERSLLRKAREALITRSLEHHLTK
                     ERILELYLNVAEWGQGVFGAEAAARHHFGKSAKELTIDEAALLAAILPSPRRYDPIRH
                     TAYLNRRQHHIVRWLERGNGRRAGSLSTNRQAPEPHDLAPSEN"
     gene            complement(16288..16716)
                     /locus_tag="PK224_08145"
     CDS             complement(16288..16716)
                     /locus_tag="PK224_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB15746.1"
                     /translation="MPALFHLAFPVHDLAAAKQFYVEGLGCVLGRESTSAVTFGLAGH
                     QLVAHLSTQSTVPQKGVYPRHFGLVLTQEEEWQALADRAKAKGLTFYQQPRRRFAGTR
                     IEHLTFFLQDPSRNLLEFKYYRHESAIFGERDVAAVGDAE"
     gene            complement(16757..17695)
                     /locus_tag="PK224_08150"
     CDS             complement(16757..17695)
                     /locus_tag="PK224_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HRB15747.1"
                     /translation="MKPSRPILLIHGGAGRRTMTVAQAACVEAALTECHRLLMAGTPA
                     LSVVEEAIRLLEESGLFNAGRGSNRQLDGVRRMDASMMEGRDLRAGAVASIEGIVHPI
                     TAARLVMEKTAHVLLVGQSASRFADYFGLERAPRAKRTAGPQPKDLRPRAGLKHTLRL
                     HQAIMQTGRLRARSLGKETVGAVALDLAGTVAAGASTGGVDVMLPGRVGDTPLIGCGV
                     YADNEAGAVSMTGLGESIIRVAVAKEISDLLASGLSPLRSANRVLRKVVERIHGTAGV
                     LVLSPDGRFAIRHSTPNMVAGVIGRDGRPHVKGRFA"
     gene            complement(17692..18258)
                     /locus_tag="PK224_08155"
     CDS             complement(17692..18258)
                     /locus_tag="PK224_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15748.1"
                     /translation="MGSPVSRSHPLRQLFGALTEKSFTEHLGWPDLNVTEYVSNLLVE
                     FAHTDQLYKIQNTQGRAVDSVVEMLFESEVLLEAQSFEREREVHRHIGDFTLFMTGLF
                     PEYLRRLKSVGRIYHKDFLVDYVKTGKRSYGLVAEYGSYDPEQDSPLFRKLSENFELC
                     VTGLGFVRSDLDRMQDPACRRVKDLLLN"
     gene            complement(18357..18827)
                     /gene="dtd"
                     /locus_tag="PK224_08160"
     CDS             complement(18357..18827)
                     /gene="dtd"
                     /locus_tag="PK224_08160"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRB15749.1"
                     /translation="MKVVIQRVTRASVEVEGRIIGRIGAGLLVLLGVAKGDDERDLLY
                     IVEKLQTLRIFGDEQGKMNRTLIEAGGALLLVSQFTLLGDTSKGRRPGFDLAALPEEA
                     RTLYEQAVGRLRAKGLTVETGLFGAHMQVELLNDGPVTFLLDSRRGASAPTLKS"
     gene            complement(18862..19365)
                     /locus_tag="PK224_08165"
     CDS             complement(18862..19365)
                     /locus_tag="PK224_08165"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRB15750.1"
                     /translation="MTHAITFQTAWGLIRITASEKGVTSIDLSPSDPAARQQPAEAGG
                     AAASIVEEARAQLVAYIAGTRREFSFPVDSSAGTPFQRKVWKAITRIPYGRVRSYQWV
                     AMRVGGKQYARAVGMALGANPVPIVVPCHRIISHDGSLGGFSCGLPLKRRLLSLEGTL
                     NQLRNAQ"
     gene            complement(19367..21073)
                     /gene="murJ"
                     /locus_tag="PK224_08170"
     CDS             complement(19367..21073)
                     /gene="murJ"
                     /locus_tag="PK224_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRB15751.1"
                     /translation="MLQMGATFKISVNDRLPLFVSLQSPIMSDPAASVEPSRPAPDET
                     RSVVKAAGLIGVATFSSRILGFVRDMVLARLFGATPAADAFFVAYRIPNLLRELFAEG
                     SMSAAFIPVFTEYHTLKTKRDAWELASATFTTLLTIVTAVTLLGILAAPGIVWLLAPG
                     FRGSPDKLALTTLLTQMMFPYLIFISLAALAMGILNSLRAFAAPAFSPVFFNIFTIAC
                     MMFLSPWLPEPIIGVAIGIVVGGVAQFAMQLPGLKGRGMLFGLRFQPGHPGVKRIGWL
                     MVPSLLGLSVTQINITVSTILASYFAGGPTYLFYGMRLIQFPLGIFGVALATAILPTL
                     SAQAARGALDELRTTIGFGLRMIFFIILPAMLGLILLRHPIVHLVFEHGSFTKADTLA
                     TATALLCYAVGLWAFAGVRIIVSAFYSLQDTRTPAVTAGIAVGANILLSLWLMTLLDA
                     AGLALATALASMLNGSILVAVLHRRLGRVDWGSIVWSAFRVLGACIPVAVMCLWVANA
                     SLWDQDGAWVVKSAALFGGIGVSVIGYLGVHIMLGSEELDVVLGMVKRKLGRVAKKFG
                     AA"
     gene            complement(21037..22626)
                     /locus_tag="PK224_08175"
     CDS             complement(21037..22626)
                     /locus_tag="PK224_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15752.1"
                     /translation="MIRGYAAQPSLLPGNRLGLHVSTTSPQFRVRFYRQGANLEYIAQ
                     SEWLRGIQAAEPATAAERTRDWNWPEYWFPIPSEWPSGVYIALLIEDVKGRLLDPPES
                     APAIDGRHGRVLFVVKNSAPGKHASILYNLPLFTYQAYNYTGRSNFYMRGNPPGPDQT
                     HRVSLRRPGAGVGGDIGGEYDGYCGSACDWYDASSPRNTFAHWDAPFIGWLLASGYQL
                     DFCTDLDLHDGTSALSSYRLLLCAGHHEYWSTEMRDRVESFVHAGGNVALFSGNTCWW
                     RVRVRLDGAQLECRKIDNGDLWWSGAHRVENSVTGVSYRNGGAWWDGAEREALGFTVQ
                     HSSSWVYEGTGMRDGETFGREQALVGFECDGAAVSEPPSTAHPLVPSFQDGTPATFQI
                     LGWAQLSKFGNGMTGWWQESNEATNPGLRVATMGVFTDGGTVFNGATTDWVRVVVKAR
                     EPRTIRITHNVLKHLSGQEAVGPAESASHAIADVFLRPVTIAGGLLAALRTPMTRRLF
                     LSSGLMMLLACSKWGRRSRFP"
     gene            complement(23135..23797)
                     /locus_tag="PK224_08180"
     CDS             complement(23135..23797)
                     /locus_tag="PK224_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRB15753.1"
                     /translation="MATGRRPTLTDEERARVEAAVQAAEQRTSAEIVPMIVGRSGLYR
                     EAHHRAGLLSAVLALTALLTIETTWLPWGWHAANAVWLLGVIMLAYALGSWVGTWPPV
                     LRLFTSRERMHRKVQLRAKLAFAQHGLAQTRERTGLLLLISLLERQVSVLADQSLRDG
                     ISDAQWKEVVAVVVERLKVGDLVGGLCCGIEASGTLLARACPARDGDNPDELSNQVIQ
                     DP"
     gene            complement(23784..24689)
                     /locus_tag="PK224_08185"
     CDS             complement(23784..24689)
                     /locus_tag="PK224_08185"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRB15754.1"
                     /translation="MRLMGRTGWGLLIGWLWLTGVASLPAAALDVPLLSGRIVDQAHA
                     MPPAVAERLSADLAAHEANTSNQVAVLIIPSLEGEPLFDFSHRVATAWKLGRKGTDNG
                     VLLLVSIKDRKIRIEVGYGLEGVLTDARSAQIIRNEIVPRFRAGDVPAGVTAGVQAIL
                     QTIEGTYRASEHPTATPSSSDAFATAAFAVILGVVVGLFFSRAHRVMGPVVGGGLSFL
                     AAPWVIPAVMAGAVSLVLVGLLSKLGPSSGSRRRGDGSAFDGTWFSTQQGGWGSGGFG
                     GSFGGSGGFSGGGGDFGGGGASGDW"
     gene            complement(24721..25323)
                     /locus_tag="PK224_08190"
     CDS             complement(24721..25323)
                     /locus_tag="PK224_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRB15755.1"
                     /translation="MFMSLTRSMGGVVLLAAVLALPGCGYNDLQGLDEDTKAAWSEVI
                     NQYQRRADLIPNLVNTVKGYAAHEKETLESVVQARAKATSVQVTPELLKDEAAFKKFQ
                     EAQQGLSSALGRLLALAENYPNLKADQGFRDLQSQLEGTENRITVARKRYIDKVAEFN
                     KMVRYFPTNLTAKFLLHLEEKANFTVADEKAVAKPPEVKF"
     gene            25486..25791
                     /locus_tag="PK224_08195"
     CDS             25486..25791
                     /locus_tag="PK224_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15756.1"
                     /translation="MALDRSTDTPGGFQVRHRALGVFQGSSIGLAFWHPSSHMPEYGL
                     CRFATEAKAQDYVDFLSSPACPEPLNRADLIVEPFDHIEHDRLTTEYPQASAWETPL"
     gene            25946..26356
                     /locus_tag="PK224_08200"
     CDS             25946..26356
                     /locus_tag="PK224_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15757.1"
                     /translation="MHKRKKSLVFLRRPKKFERNTAGTHRPDHRSHFERRLVVLKRDL
                     KIKDIVHMHRSLALNDAPAHRNVQDGSFAPDFAAREGQKQAHGNTQMFSPIEHLRRIT
                     ASQSEGLETEATRLTMERGNTSKRLQPLHQRGAA"
     gene            complement(27363..28136)
                     /gene="xth"
                     /locus_tag="PK224_08205"
     CDS             complement(27363..28136)
                     /gene="xth"
                     /locus_tag="PK224_08205"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRB15758.1"
                     /translation="MKVATFNVNSLRKRLPIVLQWLERHQPDVLCLQETKVQDSEFPL
                     TALATSGYEITFRGMKAYNGVAILSRTKPEAISYGFDDGGETEDARLLRVVIQGIPII
                     NTYVPQGFEIDSPKYQYKLGWYDRLRKHFETHLSPKEPAIWCGDMNVAPRPIDVHSPE
                     KHLKHVCYHEDARNAYGKTVAWGFEDVFCRLYPDRQQFTFFDYRAPSALAANKGWRID
                     HILATPPLAQRCQLVDVDLEPRRATDPSDHTVLWAEFST"
     gene            complement(28278..28682)
                     /locus_tag="PK224_08210"
     CDS             complement(28278..28682)
                     /locus_tag="PK224_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15759.1"
                     /translation="MRRSIAVLVAVAFVGMSSFAVADDMGLGKMSEGMKQDMSGLSND
                     LNAQKEDLASDLKAKKESVKSEMKAKRDEAKAKKKQAKADLKAKKEHMKAKVKAKKDA
                     ARAKAAEARSVADDAAHEAGGMKNDVTGAMRN"
     gene            complement(28883..29707)
                     /locus_tag="PK224_08215"
     CDS             complement(28883..29707)
                     /locus_tag="PK224_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15760.1"
                     /translation="MRELLPVIGSSVFFDLLKSILLLLILLIARTVLVRWIKGNHTLT
                     IDAKRRWIVAARNSMVLGLFVGLVVIWAHELEAFAVSLVALAATVVLATKELILCWSG
                     AALRVGGGVYGVGDRIQLGTYRGVVLEYDVFATKLLEIGPGQTSHLYTGRTVVFPNSL
                     LFGNPLIKESPSQEYGLYVLSVPLQSTDDWQAAEQALLNAAKVECASFMDQMGRQMKL
                     LEQRNLLEAPSPEPRITIQLPEAGRIHLVLRFPAPDRGRSRVEQAILRRYLIAVRP"
     gene            complement(29725..30171)
                     /locus_tag="PK224_08220"
     CDS             complement(29725..30171)
                     /locus_tag="PK224_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15761.1"
                     /translation="MVCPGRWSALLLLLCVAMLSGCGGPWRDRYLDKGVKKLTQDDIA
                     EKFGPPSTAKTPVLGGDTVWTYRVPMGDREVHPWDPGSLTAGKKTKAPPSPSPFGKSL
                     AGNDEPYREPLYCYRYTLSFDEAKLLKDWKREECVPRTSEDETASQ"
     gene            complement(30200..30415)
                     /locus_tag="PK224_08225"
     CDS             complement(30200..30415)
                     /locus_tag="PK224_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15762.1"
                     /translation="MSDLRTNALIARGMGIVFIAGGFMLGIYGLEFPNTMWLQTALGL
                     LVTGMMAQGYAMYCSIRWVREHRDSER"
     gene            complement(30492..31175)
                     /locus_tag="PK224_08230"
     CDS             complement(30492..31175)
                     /locus_tag="PK224_08230"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB15763.1"
                     /translation="MKRTVAAGASALLLLVTLACGQQTWDAAMQAGETALQRGQYEQA
                     EKIFSAAVHKAEKFGLHDRRVAVTLAHLAQAYSAQGKFVEAEPVYLEALKIYQDVHGE
                     NHLDVAAMLNNLGVLHRKHGQYADAQRLLTRALSIKEKLLGTDHPEVALALSNLAAMY
                     LAQGDGEQAGALFARALAVREKQLGADHPDVAKNLEDYASALRKLGRVGEAEGLERRA
                     GAIRAKPKS"
     gene            complement(31172..32581)
                     /locus_tag="PK224_08235"
     CDS             complement(31172..32581)
                     /locus_tag="PK224_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB15764.1"
                     /translation="MSSQLSHMPERRHDVVVIGGGSAGYAAARTARDAGADVAIVDQG
                     PLGGLCILRGCMPTKTILRSAEIIALMKRAREFGLSPVTAQADLSAIVDRKNRLVQEF
                     ADYRIAALRDPRFTLYESRAEFLSPHELRAGDVRIRGGAFVIATGSSAAEVAIPGLDE
                     AGYLTSDEMLDIRRQPASLLVLGGGLVAVEFAQFFARIGTKVTMLQRSTTLLSDMDED
                     ISQALAAAFQDEGIEVMTGVSFQRVSSTPSEKTVHFVQGGAVHTRSAEVIFQALGRRP
                     NLKGLNLKAAGVCVVDGRLVVGGDMRTSQPHIFAVGDVNDLTPIVHLAIEQGETAGFN
                     ATHPNEPARVIDHRLDAEVVFTEPQVAVAGMSERQCREEAIPYLTASYPFADHGKAMC
                     LGAVHGFVKLLCRPDDGALLGAQIVGPEAGELIHELIAVMYFRGTAQDLLRIPHYHPT
                     LAEIVTYPAESIVEQMGRA"
     gene            32631..32849
                     /locus_tag="PK224_08240"
     CDS             32631..32849
                     /locus_tag="PK224_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HRB15765.1"
                     /translation="MMKPGRYRHYKGNDYEVLGVARHSETEEEYVVYRQLYGAGGLWI
                     RPLGMFLESVTIGETVVPRFRRLEEGPA"
     gene            complement(32829..33302)
                     /locus_tag="PK224_08245"
     CDS             complement(32829..33302)
                     /locus_tag="PK224_08245"
                     /inference="COORDINATES: protein motif:HMM:NF020254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HRB15766.1"
                     /translation="MDDPNQVWSQPSVVEWSQLLLNSYRHWVGRDLMLRTGGPEEQAH
                     ALFMAPFVVVSHGAQEDPILNYGSHLALTLWETTWEQFLQTPSRLTAEAVNRAEREWM
                     LERARVQGYVDNYRGVRISNSGRRFLVESAIVWSVIDQAGRRQGQAATFSRWTFL"
     gene            complement(33394..33945)
                     /locus_tag="PK224_08250"
     CDS             complement(33394..33945)
                     /locus_tag="PK224_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15767.1"
                     /translation="MARWLGAFVVAGCLGVAGWCPAEAKDHPAAKSLMRTGLATHGHS
                     SRTCAGDDLPGNWHLVAFDSSYRFRNPQAPYLFPHQAFQYSHRGGAKSAHSLQPLSGD
                     PDQLFEAVPLDMTYRVEGKGRVVLKTRGHTETVETWTCRVITQDPEAMGDGTRLQRGD
                     LVMTLIGSNGQGLFVRHLRKRAA"
     gene            complement(34046..34279)
                     /locus_tag="PK224_08255"
     CDS             complement(34046..34279)
                     /locus_tag="PK224_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15768.1"
                     /translation="MEQEPVDAERITIPRWTIKRDPRALGCTICGSAIVKITAEWRQV
                     SFCTCRLIEYRVLPRAPKALPAPSTAADVRKEP"
     gene            34440..35843
                     /gene="leuC"
                     /locus_tag="PK224_08260"
     CDS             34440..35843
                     /gene="leuC"
                     /locus_tag="PK224_08260"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRB15769.1"
                     /translation="MAAQTLFEKIWNDHVVRAEPDGTTLLYIDRHLVHEVTSPQAFEG
                     LAVAGRTPRRPGAALAVPDHNVPTTDRRVAIADPISAKQIQTLDDNCNTFGITMFGMN
                     DIRQGVVHVIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEVRVDGQLSPRCSAKDVILAIIGKIGTAGGTGYVIEYTGSAIRALSMEGRM
                     TLCNMSIEGGARAGMVAPDDTTFAYIKGRPMAPKGALWDQAVTAWRQLATDPGAKYDA
                     VVELRAETIAPQVTWGTSPGMVTGVDGNVPDPRTMGDDKLRQATEQALEYMALLPGMP
                     IRDIKIDKVFIGSCTNSRIEDLRLAASFAKGKKVASTVHAMVVPGSGLVKQQAEQEGL
                     DRIFKESGFEWREAGCSMCLAMNADVLQPGERCASTSNRNFEGRQGAGGRTHLVSPAM
                     AVAAAVEGHFVDIRHWS"
     gene            35930..36550
                     /gene="leuD"
                     /locus_tag="PK224_08265"
     CDS             35930..36550
                     /gene="leuD"
                     /locus_tag="PK224_08265"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRB15770.1"
                     /translation="MDAFTTLTGLVAPLDRLNVDTDQIIPKQFLKTIKRTGLREGLFY
                     DWRRLKDGSQDPAFFLNQPRYHNATILLARDNFGCGSSREHAPWALLDQGFRCVLAPS
                     FADIFYNNCFQNGILPVVLTGPEIQALFEGVTAREGYQLTVDLAAQQVTTPDGASYHF
                     EIDPFRKDCLYRGLDAIGLTLQHAGRITEYEQRRRTQAPWLFQDVR"
     gene            36572..36961
                     /locus_tag="PK224_08270"
     CDS             36572..36961
                     /locus_tag="PK224_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15771.1"
                     /translation="MTWWETILFPVAWLWRPKLLFALLFFGGAAYLLIIFNWSYSDGD
                     RVGYLQKLSRKGWICKTQEGELAMTTVPGVAPVLWSFSVWDDAVAKKLDGQMGKRVVL
                     HYKEFRYIPTTCFGETAYFVDRAEVLD"
     gene            complement(36982..38040)
                     /gene="corA"
                     /locus_tag="PK224_08275"
     CDS             complement(36982..38040)
                     /gene="corA"
                     /locus_tag="PK224_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRB15772.1"
                     /translation="MKLKHKHSRKAGLPPGTLVHIGEKKSETVTISVYEYGEGQFQER
                     AVSKPDEIAMTGEPTVRWVDVGGIHKMEVLESFGKMFGLHPLLLEDIANTDQRPKLDD
                     YGSYGYVVLKMLYEGDREGDINVEQVSLVFGENFLLSFQENGGDVFQGVKERLRNGKG
                     RLRHAGADYLLYALMDSIVDRYFVILETLGERIEALQDLCVTNPGPETLRDIHGLKRQ
                     LLFLRRSVWPLRDVMNNLSRSDGQFLQGSTKVFFRDVYDHIIQIGDTIETLREMVSSM
                     MEVYLSSVSYRLNGVMKVLTIITTIFMPLSFIASIYGMNFEHMPELKSPWGYPAVLGV
                     MAATGLAMLYFFKQKKWL"
     gene            38191..39003
                     /locus_tag="PK224_08280"
     CDS             38191..39003
                     /locus_tag="PK224_08280"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRB15773.1"
                     /translation="MDHELSILSEAMRRAGREALRMAATGFDTYTKPDQSPVTSADLA
                     VNQILRDHLSAAFPEDGWLSEETEDNHNRLTRSRVWIVDPIDGTRSFVRGLPEFCLSV
                     ALVEQGMPAVAAIFNPATGEFFSAIRGQGIRVERQAEASQAPFTAPERPLALVNPWEL
                     RVGRFQALEPHLLCRPIGSIAYALALVAAGQADAVVTLEGGNEWDIAAGVLLIEESGG
                     RATTAAGHPFTFNRADPRVKGTLAIGTDLTTPTRTHLIQLSAASRDLRAPLP"
     gene            39067..39936
                     /locus_tag="PK224_08285"
     CDS             39067..39936
                     /locus_tag="PK224_08285"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB15774.1"
                     /translation="MNVALLGTGLLGQAVAERLHATGHHLSVYNRTTDKTLALRQQGI
                     RIASTAVEAIAAAEVVILLLADAAAIRAVLLTPSAREALRGRTIIQMGTIGPSESCSI
                     GEDIERLGGQYLEAPVLGSIAEAKAGTLLIMVGATPERYATWSPLLHALSSDVRLIGP
                     VGKAAVMKLALNQLIAAETAAFALSLGLIRRSGIDVENFMTVLRKSALYAPTFDKKLP
                     RLAERNYARPNFSTRHLLKDVELTLAAADSAQLSPGGLPGVRSLLSDTIALGLGDVDY
                     SALYERVDPVTGT"
     gene            complement(39946..40431)
                     /locus_tag="PK224_08290"
     CDS             complement(39946..40431)
                     /locus_tag="PK224_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15775.1"
                     /translation="MMLKRLSFMSICFGALVLQACSHAPRPQEASWAEPVSGEISLHR
                     AEHAVLAGEWEYEESGMVVPLRLDQFGNGSYDFKDGRFRTDVLSNHRWAGAWAQREND
                     REGGFEITLSPDYSEGEGRWWYTRIESDAAPTKAGGTFHVMKVQSIVENQPVPGARAS
                     Q"
     gene            complement(40837..41991)
                     /locus_tag="PK224_08295"
     CDS             complement(40837..41991)
                     /locus_tag="PK224_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRB15776.1"
                     /translation="MKKSDRTARLAQSDIRAMTLACAKVNGINMSQGVCDTPVPPVVV
                     QGAQQAMAQGHNIYSRFDGILELRQAIADKLAAYNRITADPDVNITVSAGATGSFQAT
                     CMALLNPGDEVILFEPFYAYHIQAIVAVEAVPRCVSMRPPDWTVDFKGLEQAITSRTK
                     AIVVNTPGNPSGKVFTRRELEQIAEMACRHDVMVITDEIYEYFVFDGREHVSMASLPG
                     MADRTITIGGYSKTFSITGWRIGYSVAEATWAKAIGAMSDVLYVCAPTPLQHGVAAGI
                     RALGPSFYSDLTKEHQQKRDRFCGALAKAGLPPAVPQGAYYVLADVSRLPGKTSRERA
                     LYLLDKTGVAGVPGEAFFEGPEGSRFMRFCMAKTDTDLERASQAIERFMS"
     gene            complement(42151..42768)
                     /locus_tag="PK224_08300"
     CDS             complement(42151..42768)
                     /locus_tag="PK224_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRB15777.1"
                     /translation="MQEPEWNSRWERVARALCPFVVAGCLVVMMPPVSRVAIDPGMRD
                     GPFPRASESITALITRSAAQYGLDPALLRAVIKVESNFNSGAVSSKGAIGLMQLMPLT
                     AAALHVLDPFDPKDNIYAGAALLRRLLNRFDGDLSLALAAYHVGETRVRQTLGTPALP
                     ATQLYVERVLSHYHRFRAESHQTAAHSRTRESGGEAFLASPRVRE"
     gene            complement(42808..43194)
                     /locus_tag="PK224_08305"
     CDS             complement(42808..43194)
                     /locus_tag="PK224_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15778.1"
                     /translation="MMPLLTVCGRLLFVLPMGFFVMGCTQSALQAPSPQENLGMGIVT
                     GTLGAQIPVAATAGDQPGGSLVGQVQAVEGGAYLVRDVRGQEYRIPHDQNTRIDRPAH
                     VGDRIQSWFDRQGRAVLIRSLDEEPR"
     gene            complement(43344..43784)
                     /locus_tag="PK224_08310"
     CDS             complement(43344..43784)
                     /locus_tag="PK224_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15779.1"
                     /translation="MTVDRYLLRLILVIAWLNGVHTIDHIMRGDFHWPLDGQSVGVVL
                     VVAAIYLVIGVGVRLSRREVVGPLFWAIIGCGGLVFGWLSHFSPFTDQTVHVIYGTYH
                     NAMAGALAVLCLMLLMGVLLTATLYSFYLWHRQRHITVSRPVSR"
     gene            complement(43781..44227)
                     /locus_tag="PK224_08315"
     CDS             complement(43781..44227)
                     /locus_tag="PK224_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRB15780.1"
                     /translation="MTGEGSQAWLARLIRECRTIAVVGLSSNPARPSYRVAAYMQQQG
                     KVVVPVNPRESDLLGERAYPALSAVPGPVDMVNIFRRSEEAGAVVDEAILIKAKAVWL
                     QEGVIDEAAAERARRAGLQVVMDRCWLKEHMRCVSEHGPDVRPANA"
     gene            complement(44224..44847)
                     /gene="msrA"
                     /locus_tag="PK224_08320"
     CDS             complement(44224..44847)
                     /gene="msrA"
                     /locus_tag="PK224_08320"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRB15781.1"
                     /translation="MNIVIRRGMYTWLVLAMVLAGSLASVGQAAGPAGPAKAYFAGGC
                     FWCMEEVFEKVPGVISVTSGYMGGRVEHPSYEQVSAGGTGHAESVEVVYDPAKVSYTA
                     LLDAFWHNVDPVTPNAQFCDHGSQYRAVIFYQGDEEKRLAEESKGAIEQSGRLPGRIV
                     TELTLASTFYPAEDYHQDFYKKNPVRYKFYKYNCGRAQRLEDLWGAP"
     gene            44991..45464
                     /locus_tag="PK224_08325"
     CDS             44991..45464
                     /locus_tag="PK224_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRB15782.1"
                     /translation="MRRLASRVGLVAVLTLSGCASWFMKGEAPEVLVTNVTPLDASAF
                     EQRLQVDLRIRNPNDFDLAVTGIDFRLDLNGNRLVRGLGNKGLIVPRLSDSVTSVETS
                     TSTLQVVRQLLSFSGDQPLTYHVTGVLHTKEGRLPFDSSGVLLEKGALSGSPAAP"
     gene            complement(45879..46412)
                     /locus_tag="PK224_08330"
     CDS             complement(45879..46412)
                     /locus_tag="PK224_08330"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRB15783.1"
                     /translation="MRYIVGVMGPAKARKKDVDNARVLGELIARREWVVLTGGRDVGV
                     MDAACQGAKKVPGSLTIGVLPSARERVSKYVDLAIITEMGNARNNVNVMSSNVVVVCG
                     LMGAGTVSEVALALKAGKPVILVGATPAEEKFFKKLGGRLIASVDSPEEAIGLMMKQF
                     EQQQPDLVQGARSWGGV"
     gene            complement(46415..47236)
                     /locus_tag="PK224_08335"
     CDS             complement(46415..47236)
                     /locus_tag="PK224_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB15784.1"
                     /translation="MLKTDYVFHATEEARELERLQMLERIFDPGTQRRMLATGLTTGW
                     HCLEVGAGAGSIVRWLEQRVGPSGKVVAVDTNPRFLRGSGSSTIEILQGDICDMELPP
                     AAFDLVHARYVMIHIADYQTAFERMLRCVKPGGWVVIEEPDFQAARAVAGPDEARAAF
                     GRVSEAIERMFTSLGMDHALGAKLPVLFQRHHLDQVTVEHEGHLSAGGDLIPQMMKLS
                     AEQLQAKYVATGLVTESDMEQYGRLADDPDFWAIYYATVAVTGWWQPQCGGRTQG"
     gene            complement(47294..48928)
                     /locus_tag="PK224_08340"
     CDS             complement(47294..48928)
                     /locus_tag="PK224_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15785.1"
                     /translation="MVKGRYARALVHALMVAGLALPGSGGVARAEVTLFENLGSLHHQ
                     ITTNSDLAQKFFDQGLRLVYAFNHEEAIAAFTEASRLDPDAPMPYWGVALSLGPNINA
                     AMDPKVEPRAVEAMQKATARLSQATARERAYVEALAARYSAKKSVTRKAKDEAYAKAM
                     RQVAREAPDDADAATIAAEAMMVLRPWDYWRADGRPQPGTEEILAILEAALRQHPDHP
                     GACHYYIHAVEASPDPQRALDCAKRLPSLMPGAGHLVHMPAHIYMRLGRYREASERNA
                     TAAMVDHEYLSRHPLEGNYASGYYAHNLHFLNASLMMEGRSAEALQVARDLLAKVPGD
                     EIAKEPSLEWYAPTVLLTMVRFGQWGELIRHPPPSKSLRLTTGLWHFSRGLAFAATTR
                     FGSAEGELSNLRKAMKPFARAKTAEGKISRTMLKVAERVLVGEMAARREQYEAGIQAL
                     REALQLEASLPYSEPPFWVQPVRHNLGAVLLLAERPAEAEAVYREDLRLNPENGWALH
                     GLMQSLRAQNKDATQEEARFRAVWAQADVTLTGSRF"
     gene            complement(49226..49933)
                     /locus_tag="PK224_08345"
     CDS             complement(49226..49933)
                     /locus_tag="PK224_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15786.1"
                     /translation="MKRKQQRLAEKPIHLLTFAPDKSEKGGVNPFAPSPLRAAIGKVF
                     VKLEDMTERFEDWCQYGNSEDRTEQPLGQRVSKWLGAPVARHIEHEVQEEHGLVPARR
                     WDGAVGDLIFRLRDRAGFVQRALTAQARELKEWVIEHTRSTQAEVDQLREQVSTQQVQ
                     VEELTAQLQDLRALVTSQQKVLMYMGKDMELAQPAELELSLVPSRAIPYRDRDKKSSR
                     DRREASAEASQTPYLNA"
     gene            complement(50059..51405)
                     /locus_tag="PK224_08350"
     CDS             complement(50059..51405)
                     /locus_tag="PK224_08350"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRB15787.1"
                     /translation="MTSSITPSWPKLTGRQEFEFLAELVMSHSTGDHTYLSLRDVHGG
                     TTRFANNQVIQNVNQRRGALSITVAFGRQHGTASTTDFTAGAVRETLKQAECIARISP
                     EDPEYLPPVGPQTYLSLPTSRPETSAAGPARRLDYAREAIGQCKMENLNAAGTVSSST
                     AIVGLAADTGLRAYEERTEARFSLTVQAGEATGWSAAAHRSIDRLHVQERTLAAIIKA
                     KRGADEPRELPPGRYTVILEPAAVAGLLSWMIWMLDAKSFYKGTSPFSRKLNTRILDR
                     RLSLFNQPAHADLLGHGFTIEGLPVIESSWIEAGVLNQLLHDRFTAQEHGIDPLTTLE
                     SPYLSGERPVGTRVDDLIRTTQRGILVTNFWYIRPVNPSDLTLTGMTRDGTFLIENGE
                     ITSAVRNFRFHESPLRAFNQVETYTAPVEAVTSETGKFLVPAMRIHGFNFSSVTRF"
     gene            complement(51402..52865)
                     /locus_tag="PK224_08355"
     CDS             complement(51402..52865)
                     /locus_tag="PK224_08355"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRB15788.1"
                     /translation="MSGPLWDELAELALSRVRAAGVEYADIRLLDTTTRTISGEDRRI
                     AHIRESTDRGFGIRVLHRGAWGFAASSVISLEEIPRVADLAVEIAKGSASLAITKVHL
                     APEPVHRDRIVTPCRLDPFAVPLEEQTSLLLSTMDVIQRHQGVVRSHASFWAQRDRKL
                     FVSTEGSHLEFSLLAVQGECSATAVQDGRFATRSFNSPHLRMGYELIRDTDFASEGAR
                     IAEQAVEKVRAPVIEAGRYDLVLDPEHLSLTMHESCGHPSELDRALGYEANYAGTSFL
                     TPDKRGTYRYGSSHVNLVADNTEPHTLAATGYDDDGVSCQKWDIVREGLFVGYCTNRE
                     VAPRINEERSRGSNRADSWGSVPMVRIANIGLEPGTATLDELLADVKRGIYIEGHGSY
                     SIDQRRYNFQFGGDAFWLIENGRRTHMVRDVIYHGITPEFWGRCDGVADRSHRRRYGF
                     ITCGKGQPGQSGWMTHAASHARFRRVDVIAGEAKANE"
     gene            52981..53292
                     /locus_tag="PK224_08360"
     CDS             52981..53292
                     /locus_tag="PK224_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15789.1"
                     /translation="MFSPEQTPRRRIIELLTGTLLSSHQLAQLLGMPERQVEDHLTHI
                     VKTLSRDPARTFLIEPSECQDCTYVFRERTRLTRPSRCPRCRSEAITSPRFGIREEDR
                     A"
     gene            53408..53632
                     /locus_tag="PK224_08365"
     CDS             53408..53632
                     /locus_tag="PK224_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15790.1"
                     /translation="MNRVAAWIIPSLLLVLPACGPDQAAQLLETAQFEERQTNRAHAK
                     ELYEEIVRQYPDSPAAKTARARLTELTDKP"
     gene            complement(53962..54762)
                     /locus_tag="PK224_08370"
     CDS             complement(53962..54762)
                     /locus_tag="PK224_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRB15791.1"
                     /translation="MTARDPLSLLITAWAASAALMVLLWLVERRRRNASIADVGWCYG
                     LALVVWWYAGSAAGEPARRLLVALMIFLYAVRLGTHVLIDRVWRKQEDGRYRALRHQW
                     GAHESSRMFWYFQLQAAAIAFFSLPPLVVMQNPHPPFHFWDLIGFLWWAAAVMGEAVA
                     DWQLAAFRSKPWNTGRVCRIGLWRYSRHPNYFFEWLHWWSYVWMALAIPTGGWGLTLI
                     GPMVMGVALLKVTGIPWTEAQSMTSRGEEYAEYQRTTNAFFPWFPRRS"
     gene            54948..55304
                     /locus_tag="PK224_08375"
     CDS             54948..55304
                     /locus_tag="PK224_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB15792.1"
                     /translation="MNMGFVKVLIVAIAFAAALPIQSEAGENASPKAAHPAKGKTLFI
                     KHCAGCHGAQGQGDGYKLLGPDPANLTAPATRKKSDRALLATIHEGKPNMPSWKGLLS
                     ERDIRNVLAYIRTLPH"
     gene            complement(55306..56190)
                     /locus_tag="PK224_08380"
     CDS             complement(55306..56190)
                     /locus_tag="PK224_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB15793.1"
                     /translation="MKPTALITGAGGLIGGYLVSTAARWAPQWNVRGVTRAEADLTNP
                     MQVQELWRRSTPALVIHCAALSRTGACEQDPAQARRINVEATERLAGLARDIPFIFLS
                     TDQVFDGAKGWYVETDAVHPLNVYGQTKAEAEQVVLENPAHSIVRIALTAGTSPTRDR
                     SFVEDMLRTAAKGTKLTLFTDEFRCPLPAGPLVRALWEFAAQPRAGLYHLGGSERLPR
                     WEIGELLARRYPELRPWIQPGSVVDYHGPPRPPDLSMRSDKMQALLSFRLPGFRHWLN
                     SDSSVGDDPWDGSASGDR"
     gene            complement(56187..56861)
                     /locus_tag="PK224_08385"
     CDS             complement(56187..56861)
                     /locus_tag="PK224_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRB15794.1"
                     /translation="MTTPVKTRCVFVTGATGYLGARLIPLLLERGHQVTALARQESVC
                     KVPTGCQIVVGNPLESGTFVEQVRGADTLVHLVGVPKPAPWKGSQFRAIDGPSAVAAI
                     RAAREAGVRHMVYVSVAHPAPVMRDYIAVRSECEAAIAGAGLVATILRPWYILGPGHW
                     WPLALQPVYRLMEQFPGTRAAALRLGLVTIHEMLMTMLWAIEQPPSGSRVIEVPEIRR
                     LGRSDA"
     gene            complement(56858..58114)
                     /locus_tag="PK224_08390"
     CDS             complement(56858..58114)
                     /locus_tag="PK224_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB15795.1"
                     /translation="MSMLRERTLSRWLAVLALASASCTAIDPPPASPPEFRPLHTRAT
                     LHLGEKELADGRFVGLAFSGGGSRAAVFGAAVMKELDRLGVLQQVDVLSAVSGGALPA
                     ASYALEGYRDFNFQNGFAELVGRDFQGAMLGPWYTVPQNAIRYALTDRVPAEQVIRVL
                     DDRLFRGATFADLNPSQPILLLNATDALTGDPLVISDERFAALGQPLAPFSIARATYM
                     SAAYPGVLEPLAIPYGQAEGAPSGQPPLLAYDGGAADNLGIRTLMQVVNEALSEQSMA
                     DRFPRGCLVVSIDATGRQLNERRKPLSAAAALLRGHRRNVLELAGIPVSQQDASMFGT
                     FQVGMNGHGGTCRFWHIALRQLPDSDPLGDRVTHIKTNLGLSAEDQAALATAAARLVA
                     KGREEMHQADGWAGFMSARPALLTHP"
     gene            complement(58390..60072)
                     /gene="ettA"
                     /locus_tag="PK224_08395"
     CDS             complement(58390..60072)
                     /gene="ettA"
                     /locus_tag="PK224_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRB15796.1"
                     /translation="MATNDKQVIFSLVGVGKVYPPKKQVLRDIYLGFYYGAKIGVLGL
                     NGSGKSSLLKIIAGTDPNYVGEITRSKGYSVGLLEQEPQLDLNKTVKEVVEEGKKELV
                     ALLHEYEAVSNSMGEAGPDEMEKLIDKQAQLQEKIEAANGWELESELEIAMDALRCPP
                     ADQKISVLSGGEKRRVALCRLMIQEPDILLLDEPTNHLDAESVQWLEQHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGSGIPFQGNYTAWLEQKQDRLEKEEKAESKRKKTLEH
                     ELEWIRMSPKARQSKGKARLNRYEELVNQKQDQLAADLEIYIPPGPRLGDLVVEAKGI
                     SKAFGDNVLYENVEFSLPKGGIVGVVGPNGAGKTTMFRMIIGKEKPDTGTIRIGETVK
                     LGYVDQDRSLDPNKTVYEIISEGQDTIMLGKAEVNARGYCARFNFAGTDQQKKVKDLS
                     GGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRALEEGLEGFAGCAVISSHDRWFL
                     DRVATHILAFEGDSKVVWYEGNYSEYEADRKKRLGKEADQPHRIRYRKLTRN"
     gene            complement(60130..60936)
                     /locus_tag="PK224_08400"
     CDS             complement(60130..60936)
                     /locus_tag="PK224_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HRB15797.1"
                     /translation="MSSTQQHNSGPDSDGPEVPEPSHAERAKTLVYLQQTGGLSTISR
                     KQPGWPFGSVMPYGLDDQGQPLFLISTMAMHTQNLLGDPRASLLVTPPESRTDPLGAA
                     RVTLMGSVTRVPKEESAPVRERYLARHANAAYWVDFNDFGFFRMAIADIYFVGGFGSM
                     GWVVPSDYTAAAVDPLADQASGLIREMNEQQTATLLLLARVYGKLEAQQVTMTALDRL
                     GFHLRIKTAERMQGGRVAFTNPVRTAAEVRVGLADMAARAKAGAELVHSL"
     gene            complement(61036..61203)
                     /locus_tag="PK224_08405"
     CDS             complement(61036..61203)
                     /locus_tag="PK224_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15798.1"
                     /translation="MLVLIHKECGGPALDESPVGEVCAIPVSQFPFTCFTCLEDILDE
                     SEVRLTEDLGI"
     gene            complement(61214..61960)
                     /locus_tag="PK224_08410"
     CDS             complement(61214..61960)
                     /locus_tag="PK224_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRB15799.1"
                     /translation="MDPFTLTGADGTTLRGDRVVGKDRQLLFITGFLSKRWGNKSKAL
                     AQWCQERGWGFCCFDFRGWGESDGAWGEYRLLQWLEDAEAVTNLLAGGPPVTIVGNSL
                     GGWLAWLVAQEQPAVEELILIAPAFNMMDLRAAQISNERREQWQATGSMPWDDEPMHR
                     EAPIPWHWVEDSQALWSRRLTIPRRVKTTILHGLQDTVIKPDGSWTFVQHLLSQDPEF
                     PIDVLFKTGDHRLSSPDHLETFRQLVSRSR"
     gene            complement(61968..62681)
                     /locus_tag="PK224_08415"
     CDS             complement(61968..62681)
                     /locus_tag="PK224_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15800.1"
                     /translation="MRMDTLGGLTVRLTGGIDDKGGGNGPLVVLLHGFGAPGDDLVPL
                     SEYLDAPTGTRFLFPEAPISIPMGFGDSRAWWMIDMARIQADRAAGKVRDMSGEIPRG
                     LTEAREHMKVLLDEIQKKLGADPKRTVLGGFSQGAMLSCDALLHSTQPYAGLIQLSGT
                     LVAKQEWAPLLTKRKGLPLFQSHGTQDPILPYAMAERLRDEFLQAGVSVDWQPFRGGH
                     EIPEPVLRKLGSFLLKAVR"
     gene            complement(62813..>64915)
                     /locus_tag="PK224_08420"
     CDS             complement(62813..>64915)
                     /locus_tag="PK224_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15801.1"
                     /translation="HPERWDALEMRISRYSYLRGLDGLTPGASKKSGEAENDKKSGRS
                     NSNDKKHQDKTFEGLERPDGESQLDYLRRLVARMKQDADEASGSIGKPPKAIGVLGSD
                     VYDTLLILRAVREEFPNAIFFTTDLDARFFQERESKWARNLVVASPFGLNLHSGIQHD
                     IPPFRNSYQTSGFFTMLRALGHIRSVRSSTPSTMVCPETGFESMELLFDRYEIDLSPK
                     PFSALLCPRLFEVGRHGPVDLSADRGAVPSRVGEIEMIQPPRELFPLGMRPWWKFLNA
                     DYRLRFRHTVFWLLVAVLVSLVVFTQLPGKMWNWAKGDVAERVKWHWRVVALTIIASV
                     GAFTVVGGVDRLIRVILEDRWEGEPFSISDGVSTWPSIIIRSLVIAWCLGCLVRAYYA
                     LRSSQSELKEEFALPSYRPKGSLREQVWVLLRSIIWIKSVPQPTDGSASIARIWHDYS
                     RAALYRNRVVRSLSLLVIYLFLIFIILAVIASPGPPSSPCRGITNCKIVAGVTWAAVL
                     SMVFLNMFVFDAVLLSQSFITRLKTTSIRWPDKTLAKFHSRFELDRRYLPGLIMVRCI
                     ADQTHAVHKLVFCPFIALFFMILSRNRLFDNWDFPLALIMIWLIYAGLAAGSTIILRS
                     RARQARDLAIGYFREHLLAVTGMGTQQKVCADQLRLALEDIEHVQKGAYTPLVLNPVF
                     SASILAVISFLQYWYLGQ"
BASE COUNT        13573 a        20754 c        18176 g        12412 t
ORIGIN      
        1 gataaaccac ccgcccgtcc ccgcccctgc aaaactttac gctttgcttg gcaagtctcg
       61 cctcttcctc aatactaagt cacagcatcg attgccctta gaccgaactc gaaacgcttt
      121 ccctcctggt tccgccaagc agttccgccc cagggcgttc atgacttgga ggaactccgc
      181 aacgatcgga gtctgtgcat gaagtctcgt ctgaccgcag ccctgatcgg cgtggcgctc
      241 acactgaccg gcttactggt ccttgaaggg gtatcgcgag ccctgctgac ggcgcaggcc
      301 gatctggccc cggagcagcc tgactggtat cgatacacgc cggatttggg gtgggaacgt
      361 cgcccgcact tcaaggggct cgttgcggga gagatgcatc ggcatgagcc cgctcgatac
      421 ctgcgtgaat tcgatgcgca ggggctcttc gccgtcgata cggcacagat cggcgatacg
      481 acccacaagc gtatccttgc gatcggcgac tccaatacct tcgggtgggg tgtgccgacc
      541 cgtaatgcgt acccggaagt gctggacgat ctgcgcccgg atgccgacgt gatcaacctg
      601 ggcgtaagcg gatacagctc tttccagggg tatacggcac tgctcaaata tttcgaccgc
      661 gtccgcccgg atgtcgtcat cgcctcgttc agtttcaacg atcgtcgagt ggtgccctcg
      721 gaagcggcca ccgacagcca cgaaaaattc gagcgggagg cactgctgca tcggatggac
      781 ttcagagaga agatctatct gtaccgaggc ctccagcacc tgctgatcag attacacctc
      841 atgaaacgaa gcggcgacgg cagcacgacc gttgacgcaa gaacagcgcc gacccgggtc
      901 tcgccagacc agtatcggca gaatcttgag cgcatcgcac gattctgcca ggaacggcac
      961 gtgccgctgg tcttcgttgc gtttcaggat aatccggctc actccgaaca tctgcgggca
     1021 ggcatcgccc acctgaatgc cggacggcag gcacaggccg aagcggaact gcggattgcc
     1081 gtgaacatgg acaactggtt ctcggatctc gccaggaaat acctcgccgt ggtcctggaa
     1141 cagcgcgggg cgtcagaaga agcgacgatc acggcgagcc tcaccctccc cgcctggaaa
     1201 ctgacccacg gcgggaggcc gctccgcctc gacagtgaat acaacgacat cgtgcgagcc
     1261 gttgcccgcc ggcatggcgc tgtcgtcgtc gaagcgggtc aggtcctcgc ccaggacgcg
     1321 agtctctacc tcgacctcgc ccatccggat gaacgcggcc atcgcatcgt tgcgacgctg
     1381 gtgaataatg ctctcgacag cctgctccac ccgccccagg tcgcagccca gccttgagtc
     1441 aatgacaccg gttggtagcc tcaacgtccg aacgtgggtg gaaattacgt tcgttgctcg
     1501 acgcgtcgtc attccgccca tgagataggg aataggcgct gcaacaacgg tgggaacatg
     1561 cctttgcggg aggagaggcg aggagggctt acggtttctt ctttttcttg tcggcgggat
     1621 tatcggaaaa gagcaaccag gcgaggacga cgaaaccgat cgccacacca gtcactgcca
     1681 gccatgtgcc gagtttatcc attagctcct cgtgtctgga ggtccttgta gcgcagccaa
     1741 ggtgggtttg tcgagccgct ttactttcaa gttgtcatcc accgtcacca gtttggccga
     1801 gccttcgacc accacgcgcc cgctcgtccg ttctctcatg acatgggcaa aggtgagaga
     1861 agcctggccc accgccgcga ggctcgtgtc gatggttaag gtatcaccat agcgggcagg
     1921 ggaccgatag tccagttctg catgcacgac cataaactgc gtcccgccgt cccgtaactc
     1981 tgcaaccgac aggccccgct cttcaagata ccgcgtacgc gcccgctcga aatacttcag
     2041 atagttggcg tagtacacca cccctccgca gtcggtgtcc tcgtagtaga tcctaatttc
     2101 catggaagca gatcattgaa actgatagcg gaccgcaacc agttcttaca gattgagaac
     2161 cggaaacttc ggcaacgagg actcgttcac gcccgtgagt ttcatgacca catcggacag
     2221 ttgctgcacc cgctcggatt tgatggcctc aaacccgtgg cccaacaccg catggttggc
     2281 ggcgtcgagc agcggcttca tcttgggcca atcgcgaaga aaggcctgcc ccatttgatc
     2341 gccaagaccg gccagggccc ggaactggct ttgcaatggg agcttatact tcccgtccac
     2401 atcatcgagg taacaggtgt ggcaggtgtc ctgcatcgat tcgggcaatt gctccggctg
     2461 cacatcccag gacttgatct tgtactgttt gtaaagttgc cgctgggcgc aggcttccat
     2521 cgcgcgcacg agcgccacca tggcccgttc atagtcatgg tccacgtggg cgcggcgatg
     2581 ggcatgcgcg agtaggtcga gcaccacacc ttccttcacg tcggcaggat ccagcaccag
     2641 cttttccaaa aatccgctat tggctttgat caagggtaga agcgctttta accccggcgg
     2701 cccgccccag agcgacgcca tatcgagcgc cttcaacgag gtcttcagct tgtcccatgc
     2761 ctggcggtag tgaaactgct cccacaaccc ataccctgat gccaattcgc cgagggcacg
     2821 atagagaggt ttctgccccc cgctcacgcg cgcttccagg gtatgaaaca gcgtggcggc
     2881 ggctctgaat gaaccatgat tgaaggcgtc gctggcctca cggcgcacca cgaccgccga
     2941 ctcatcccag ggattgcctt gcagccaata cttggccaac ccgccgatcg cgatcgcctc
     3001 accctcttcg cggccctccg catcgaccag actgatgact cgcgacgtcc agggttggct
     3061 caccatcgcc agcgccgcgg ccatggcggg agtcgctccg gtcaaatcga ccaccacctc
     3121 gcccacctgc acggcccaat ttcgaaagag gtcgtgaata gatcgtgaca gggcctgatg
     3181 gcaggcaatg acatcggccg gatcgggcgt caccacccag tcccagcgct tcggcatctg
     3241 ctgtacgagc ggttgcaccg cctcctcgac cagcgtcttc gccgattccg gcacgaaaaa
     3301 gcagagcaat tccggctgca gccggttgat cacatacgcg gcgagcgacg gcgcgtcggt
     3361 gaaggcgagg atgagcgctt tgacaggagt atccgcaggc atgaccgcgg actataccac
     3421 tgcactccgg ctgcttcaac gaacgacgct ttcctcacag ttcttgacgg tcccgggaaa
     3481 gacgcataga cttccgcgtg aatcacgaga caaccccgct gcccgtgacg gctgaggagg
     3541 tgcgatgacc tggtggctct gggcctttct gggactgttt ctgctcggca gcgaaatcgt
     3601 cacacccggc gggttttaca tgttgttctt cggtatcggg gcgctggttg tcggcgcgct
     3661 ggtcggcctg ggcatgattc acagtgaatg gatgtcctgg ctcctgtttt cggtcttctc
     3721 ggtcgcttcc ctcgtcatcc ttcgtccccc gctccgccgt ctcatgacgg ccgaccgggg
     3781 caacgtctca tcggtcgata ccatgggcgg cgaaaccgcc atcgtcctgg acgaccttcc
     3841 tcccggcgcc acgggcaagg cggaatgccg gggcagcacc tggaatgcgc acaacgccgg
     3901 cgacaagccg ctgctcaaag gacaacgcag cctcgtggat cgcgtagacg gcatcaccct
     3961 gtggatcaaa cctgaatgag gtcgcaccat catggaggat agcggaatgc caggcggact
     4021 ctgggttgtc atttttcttg caggcctcgt cctgctcgtc atctcgaaaa ccgcgcgtgt
     4081 cgtgccgcaa cagagcgcct acgtcgtcga gcggctgggc cggtactcac ggacactcgg
     4141 cgcggggttc cacatcctgt ggccgttcct cgacagtgtg cagtacaaac actcgctgaa
     4201 agaaaccgcc atcgatattc ccgaacagat ctgcatcacg cgcgacaacg tacaggtcgg
     4261 cgtcgacggc attctctatt ccaaggtgct ggatccgcag cgagcctcct acggcatcag
     4321 cgactatcgc tttgcgatca cgcagctggc tcagaccgcc ctccggagcg agatcggcaa
     4381 aatcgaactc gaccgcacct tcgaagagcg caccaatatc aacagccagg ttgtgaatga
     4441 gctggacaag gcgacggaac cctggggcgt gaaggtgctg cggtacgaga tcaagaacat
     4501 cacgccgccg aaagacgtgc tggccgcgat ggaaaaacaa atgcgggccg aacgggaaaa
     4561 acgcgcggtg attctgacct ccgagggcga acgcgacgcc gccatcaacc aggcggaagg
     4621 cgagaagcag caggtcatca aggcctccga ggcgaagaaa cagcagcaga tcaacgaggc
     4681 cgagggcgca gcctccgcga ttatggccat cgccggcgcc acggcagacg gactccgcaa
     4741 agtcgccgaa tccacgcaaa ttccgggcgg ctacgaagcc gtgcagctgc gcgtcgccga
     4801 gcagtacatc accaagttcg gggaactggc caaagccagc aacaccctcg tgctccccgc
     4861 caacgtctcg gatgtcgggt cgatgctgac gctggcgatg aacatgatca cgaaacggtc
     4921 ttcgcccaca tctccgggaa agtaaaagac gtctgcggtc gtttgacagt gactcggggc
     4981 ttccctacaa ttccggacga tgaaagaatt tgccatcaag gccttcgacg acagcgccga
     5041 gatcaagcgg cgcttcgcgc gggaccatgc cgaccgaatc gtacaggtcg cacagctcat
     5101 cggcggcgcg tttcaggccg gacataaagt cttgctcttc ggcaacggcg gcagctccac
     5161 cgatgccgcg cacatcgccg ccgagtttgt cggccgctat aagcgcgaac gtgccccgtt
     5221 gccggccatt gccctcgcca cggatattgc cgccatcacc tgcatcgcca acgactacgg
     5281 cttcgaggag ctgttcgccc gtcaggttca ggcacacggg cagcgtggag atgtggcgat
     5341 tgcgatcagc accagcggca actcccccaa cgtgttgaaa ggggtcgagg cggccaaagc
     5401 cctgggcctc accacgatcg cctggaccgg cggaaccggc ggcaaactgg ccggcatggt
     5461 ggactacgcc ttcgtcgtgc cttccacgct gaccgcccgc attcaggaaa gtcacatcac
     5521 gctgggccat gtgctctgcg aactcattga ggatcacgtt cttgccaacc cggcgtaagc
     5581 gccccactgc agcatcgacg ccccccgcga tcatccggcc gatcgatgcg tcgcgcctga
     5641 agacctatcc gctccagcgc cgacacagca aggtgcaggt atcgaacttc gccaaggtct
     5701 ggacgaaggg gcgctcgttc gcgcggtttc ttgattcgct ccccgatatc ctggcagtca
     5761 agacgctgcg cgaagtggcg caggccattg ccaaagccca tcgcaacggc cggccggtga
     5821 tcgtcggcat gggcgcccac gtcgtgaagg tcgggctcgg cccgctcctg gtggacctga
     5881 tggaacgggg catcgtcacc gctctcgcca tgaacggagc cgtgatcatc catgacttcg
     5941 aactcgcgtt tatggggcac acgtccgaag aagtggacgc ggagatcgac tccggccgat
     6001 tcggcatggc ggaagaaacc ggccgcatgc tgaatgaagc catcaccctg ggcatgaaag
     6061 aaggtcaggg gcttggcgaa tcgctcggca gttatattca tcgacgaaaa caactgtttc
     6121 cacatcgcgc caccagcctg ctggccaccg gcttccggct gggcctcccc gtcaccgtcc
     6181 atgtggccgt cggaaccgac atcatccata tgcatccgtc cgcagacggc gctgcgatcg
     6241 gagcgggttc gctactcgat ttccgccgcc tggcagctgt tgtctcagag atggaaggcg
     6301 gggtctatct caatctgggg tcggccgtga tcctgccgga ggtcttcctg aaagccgtct
     6361 cgctcggacg caacctcggc catccgttga cgaacatcac gacggtgaat atggatttcc
     6421 tcacgcacta tcgccccatg accaatgtcg tacgccgtcc cacccagaaa ggcggcaagg
     6481 gatacgcatt gaccggccat cacgagatca tggtgccgct gttggccgcc gcagtcattg
     6541 aagaattgtc cggccgctga ctctggccat tcagctttcg ctcgactgtt caacatcacg
     6601 atcatgttgt gagtgccatg tcaccctctc cggaaccgct gctcacaatc tggagcgatc
     6661 tcaaccgccg ctattttcag ggcaccttac ccccgattcc cattgaatgg agccgccgac
     6721 tgacctcctc agccgggatg tttgtctgcc ggatcggacc ccgacaacca ctcgcgcgca
     6781 ccaccgaggc accaaccaga cggcgctgca tcagactgtc ggcggtgctg ctcaaaccca
     6841 acaatccgga ctcgagccac gacaccgtca tgaccctggc gcacgaaatg attcaccaat
     6901 ggcaatacga tatcttgaag cgtcgcccga atcatggtgc cgattttcgt cgcatgatgg
     6961 gccagatgaa tcgcgacggc ctgggcatca cgatctatca ctcgctggga aaagaagtgg
     7021 ctgccttggc caaatatgcc tggcgttgcc agcactgcgg ctgcctctat caacgacaac
     7081 gccggaccat ccaaccgaga cgccacttct gcggcgcctg tcgcgggccg ctccgcgaac
     7141 tgcctgccgt ggtacccgcc tctacagcgg caccggccca cacatctgcc gcaggtcctc
     7201 agctcggctt tcccttcacc cccgtgtaga acatcgatgt ccggacgatg gccccgacaa
     7261 attctcttcg gcgacatcga cgccatgttt gcgtcggcag ccgttctcgc tgatccgtcg
     7321 ctggcaggca aaccggttgc cgtgggcgga cctccgcctc gcggcattgt ggcagcggcg
     7381 agttatgagg tgcgtcgctt cggggtgcga tcggcgatgc cgaccattca ggccatgcgg
     7441 ctctgccctc agctcgttct cgtaccacca gaccggccgc tgtaccagcg tctgcaccgc
     7501 cagcttcagg acatcaccga ccggtttttc ccggaaaccg agtggacaag tatcgatgag
     7561 ttttacgcgg atacgacccg attgcaaacg cggcatcccg atccgcgtga gttggggcag
     7621 gcgctcaaaa tcgacattac ccgcgtcacg ggcctgacct gcaccattgc cctggcgtcc
     7681 ggcaagaccg tcgccaagat cgccgccgat gcgcataagc ccgacgggct cgccgtgatt
     7741 gaaccgggct cggaagcggc gttcctcgcc ccgctgccga ttcgctcctt gccgggcatg
     7801 ggtcccaaat ccactgaagc catcgagcgc ctgggcattc acaccgtcgg cgatctgctc
     7861 gaaccccgct tcgaacaatc cctcatccgc ctgcttggct cgcggctggc ggcgtttcaa
     7921 tcactcgctc gcggccacga ctccgatccc gtggtggccg atcgcgaggc caagagtatg
     7981 agccatgaga ccaccttcga cgaagacacc aacgatccgg ccttgctgga accgatcatc
     8041 cacggatttc tcgaaacgtt aacgcacgat ctccggctgg agggtctggc cgccgggtca
     8101 ttcaccgtga agctgaagga ttcgcacttt cgcatcacca cgcggcaacg gaagttctcg
     8161 tcaccggtga actacgatcc ggatatgtgg ccggacatcc gcctcgccct cgccgaactg
     8221 ctgcagccgc gatcgcgcta tcgcctggtg ggcctcgggc tgtcggatct ggttcctgct
     8281 cctgaacccc tgttcgaccg gcggcagcgc gacgcggtcg ccatactcga ccggttgatc
     8341 gagcaacatg gcgcgggggt ggttcgactg ggaggacttc cacagggcgg ggacagccca
     8401 aagaaggggg gacgtcgcag ggaagactga cagccggatt gcggtaaggc cgtcgtggat
     8461 tcgtggtggc agactttttc cacatcctgt taagatgagt ttttcgcagg cgagaccacg
     8521 atgcacacca aagtcaccac gcgacaagaa ctggcctcac gactggaaga gcgccgtcgg
     8581 cagggacaac gcatcgtctt cacaaacggc tgcttcgacc tgatgcacat cggccatatc
     8641 cgctacctgc aagcggctcg caatctgggt gatgtgctgg tggtcggcgt gaattccgac
     8701 gagtccgtgc gcgccctgaa caaaggcacc gatcgtccca ttgtgcccga tgcccagcga
     8761 gccgaggttc tggctgcact ggcctgcgta gactatgtgg tgaccttttc ggaaccggac
     8821 cccggcgccc tcatcgcaac cctccagccg gatattctcg tcaaaggtgg agattggtcc
     8881 gttgaccgga ttgtgggacg ggagacggtc gaggcccgcg gcggacgcgt ccagaccatt
     8941 ccgctcgttc ccggagtgtc gacgaccacc ctggtgcaac gtattcgctc cacgacagcc
     9001 taaggatatt gtgcctcccc tgatcccctt acatgtgacc cagtggctcg cgccggcgcg
     9061 agaagccctt cgcagcggct cgggcaaacc ctgcctcatg gggttgcatg gctcgaccgc
     9121 cggcttcggc ctggcgctcc tcacgcacag cgtgccgtct caacccctgg ccgaccgttc
     9181 ctggctgatc gtcgccaaaa acgatgacga ggcagagcgc ctctatcgcg atacgctgtt
     9241 ttttagaacg ctctgcggac agtccggaga cgacctggcg ctgttcccga aatgggagac
     9301 gctgccctat gaatcgacgg tcccccacat cgatctggtg gcccgccgca tgcagacgct
     9361 caaccgcctc tgcaccacgg cgcgcacggt gctcttcacc tccatcccgg ccttaacgca
     9421 gcgggtgctg ccggcactgg tcttcaccga cgcgattttg cagtttcaac cgaatggttc
     9481 cctggagcgg gaagtgctcg tctccagcct gttgcggttg ggctaccgga agggatcggt
     9541 ggtagaaatt cccggtgagt tcagcattcg cggcgggatc gtcgatatct attcgaccgc
     9601 ctatgccgac ccgctacggg tggagtttct cggcgacacg atcgagtcga tccgcttctt
     9661 cgatccggcc acacaaaaat cgaccgacaa gatcaaacag gcctgggtgc tgccggcccg
     9721 cgaattgatt cgccccgagg acgcgcccga tgccctcgcg cccctggccg ccgacgccga
     9781 atggcatgcc ccatcggtct acgggaccat ggacagcctc ctcgactatt tccctcagcc
     9841 gccggtgctc gtcctggatc aacccggcac gctgaaagct cacacggagg aatgttggca
     9901 ggccatcgaa gaaggattcc tgcgtcacga agatcgcaca gatccgaatc cctacccgac
     9961 accggacaac ctgtacctca cgtgggacca ggttctcgcc gccacccaag gctacgccac
    10021 gctcgcgctt gagcctgtca ccgcaccgga cgcgagttgg gacccggtgc tgacctgccc
    10081 ggctcaaacc cccgccagcg tgggactcgg ccaacgcggc accgcgttca gtcatacgct
    10141 ggaaatgctt gatcgcttgc gcgaaggcgg acccgtcgtc ctggtggccc gcagccaagg
    10201 ccaggtcgga cgcctgctgg cgttatttgg cgaacacgac cgaccggctg tggaatggaa
    10261 gccctcggcg ctctcggccg gcggcgcgca aaaggccccg ttttcggtct tgaacggcga
    10321 agtctcagct ggttttcttt ctcccgatct gcgactggtg gtcctgaccg aagaagaact
    10381 cttcgcgaag ggcgcccgcc acaaaccgca gcccaagagc aaagccgcca ccttcctctc
    10441 ctcgctggaa gatctgaaca tcggcgacta cgtcgtgcac atgcaatacg gcatcgcgaa
    10501 gtatcagggg ctgcgtcggc tctcggtgca agacttcgac agcgattttc tggtgctgga
    10561 attcgccggg accgacaagc tttatgtccc gctcgatcgg ctcagccagg tccaacgata
    10621 cgcgggagcg gatgcccacg taccccgcct ggaccgcctg ggcggcacta gttgggccaa
    10681 gacgaccgcg agggttaaaa aagacatcga agaaatggcg cacgagctcg tcgatctcta
    10741 cgccaaccgc gagctggttc atcgcacctc gtacggtaaa gacagcatgc tctaccacga
    10801 gtttgaagcg gccttcgaat acgaagaaac gccggatcag cgcaaggcga tcgaagacat
    10861 tgcaaacgac ctggcctcga ccaagcccat ggatcgcctc gtgtgcggcg acgtgggcta
    10921 cggcaagacc gaagtcgcca tgcgagccgc gttcaaggcc gtcgaggaaa atcgacaggt
    10981 ggccgtgctg gtgccgacca cgctgctcgc acaccagcat tacgataact tcgccgaacg
    11041 gtttgcgccc tttccaactc gcgtcgccct gctgtcacga tttcaatctc cgaaggacac
    11101 gaaagcgatc gtcaaagata cggaagccgg cctggtcgat gtcctcatcg gcacccaccg
    11161 gctgctccaa aaagacgtga agttccgtaa cctcggcctt gtcatcatcg acgaggagca
    11221 atggttcggt gtgaaacata aggaacggtt gaaacagctg cgcacccagg tcgatgtgtt
    11281 gaccctgaca gcgacgccga tcccacgcac attacaaatg actatggcga gtgtgcgcga
    11341 cctctcgatc atcgatacgc ccccgtcggg acgactggcg atccgcaccc aagtccttcg
    11401 cttcagtgaa aaggcgatcc gtgaggcgat tcttcgcgaa ctcggtcgag gcggacaaac
    11461 ctactttgtc cacaatcggg tggagaccat ggagcggatg ggctcctggc tccaggaact
    11521 ggtgccggaa gcccgcatcg tcatggcgca cggccagatg gactcgaaac cgctggaagc
    11581 tgtgatgctg aaattcttcc accgggaagc ggatatcctc attgcctccg ccattatcca
    11641 atcgggcatc gacgtgccga ccgccaacac catcatcgtg aatcgcgccg acacgttcgg
    11701 cctcgcccag ctctatcaac tccgcggccg ggtcgggcgg ggcggcgaac aggcctacgc
    11761 ctacttcctg gtgccggatg aaggcaacct ctcggaagat gcccagaagc ggctgaccgc
    11821 catccaacag tttaccgaat tgggatcggg cttccgcatc gccgcagcgg atcttgaaat
    11881 tcgcggtgcg gggaatctgc tcggcaaaca gcaatcgggc catattgccg ccgtcgggct
    11941 cgatctctac ctgcaaatgg tggaacaggc ggtccaacgc ctgaagggtc aggtggtgga
    12001 ggaagaaccg gatcccacgc tgcgtctcag cgtctccgcc tatattccag aagactacgt
    12061 ggccgacagc caccagcgcc tgtcgctcta caaacgacta tcttcctgcg gacaactcgg
    12121 cgaccttgcg ctgatgcacg gagaaatcga ggaccgctac gggcatccgc ccgatccggt
    12181 cgagcgtctg ttcgagctca tgcaaatccg gctactggcc aagcaactcc ggctgagctc
    12241 tgtggtcgag caaccgcatg cggtcgtgat caccttcgat cccaaggcca cggtctccga
    12301 aaccgccgtg caagccctga tggaccgata caagaaacga ctacggtttc tgtcgccgct
    12361 gtcttttgaa ctccagatac cgcatgaaga ctggagtttg gtctttccag aactcaacgc
    12421 aaccttgcaa accctccacg tctgtggtac caacaatcaa gggccacgca cggcctcgac
    12481 ctgaccttcg tagaggatgc tccgccccga ccgcatgacg ccaccgcgca cacgacagat
    12541 ttcatcctgg cagacacgat tgaccagcgc cgcgctgggt ctctgcgcgc tcggatcgtt
    12601 ggccgcctgc accgaaccac cgcaggaaga gccggtgatc gcgatgatca acggccgatc
    12661 cattactcaa tccgaattcg acatccgctg ggaagacctg tcgcaggcca cacgcgcgcg
    12721 ctacgaaaag gaaggcggga aacgccgttt tttggatgaa ttgatcatgc gcgaactgct
    12781 gatgcaggaa gcgcgcaagc agggtttgga tcagtccgat gagattcgcg aaaagaccct
    12841 gcgctaccgc gagcagctga ttctggatga gttgctcaaa gaccggatta agaccaaggt
    12901 cgaggtcagc aaagaggaac tggatgccta tctggggaaa cacgccaatc agctcctggc
    12961 caaccccaag gtgcaagtgt cgattatgct gctgccgaac gtctatgcgg ccaaggacct
    13021 caagcggcag gtcgaagccg gcggcaattt cacgcgattc gcgctgcggt attcgatcga
    13081 tgaacgcagc cgcgccaagg gcggcgatct gggcccgtat aaaaaaggcc tgctggaacc
    13141 ggaactcgat gcgttgattc cttctctgca ccccggggtc atcagtgatc cgatcaagac
    13201 cgcgcagggc tattacctcg tgaaggtcag ccccctggag ccggaaattc tgcaggcaga
    13261 ccaggccacc cgcgaacgac tccgccagga actgttggcc gagaagcgcc ggaaacggct
    13321 ggacgacgtg tttgccgaac ttcgcaccgg tgccacgatc cgcctggccg acgcagctcg
    13381 ttatgtgacc gatgaaccag cgcgcccgta accgcccgcg cactcccctc ctgcatactc
    13441 tccgtcttca cccgtccgcg tttttcatct ccagagcgct ccctttctga agcctcgatt
    13501 gtgtacaatc cagcaaccct atgaacgatc aatcgctgtc cgcccacacg tgcctccacg
    13561 gctaccggtt cttcttgtcc cagaagggca ggcctgcccc aggacccgtt cgcagtgcga
    13621 ccgggttcat gaccacaggc gcgctctttt tctgcctctg cttactcttc gtcctgatcg
    13681 ccaacgcggt ggccgccaag ctggaagatc gcattgtcgc ggtcgtcaat tccgacctga
    13741 tcatgctgtc cgaattgaag cgcgacctgc ttcccgagca agaccggctg cgcaagctgt
    13801 acaaaggcga agacctggag cgccgcctca agacggctga ggccatgggc gtcacgaaaa
    13861 tgatcgagcg gaaactgcag ctgcaggccg cgaggaacaa aggtgtggac gtgtccgatc
    13921 aggaagtcgt gcaggcagta gaagagatga agaaacaggg cgaggccatc gacagcgccg
    13981 acccgaacac cgccagaagt attcgcgaac agttgacctt aatgcgagtt gtcgaccgcg
    14041 aagtccgcgg cctgattatg gtagcggact cggaaatgaa acggtactac caggagcacc
    14101 aggatcggtt tgcctacccc gaggaatacc aactcagcca aatcctgatc aagccgcgca
    14161 cgccggacgg cctctccgcc gcccatggac gagccgaagc gctcctggca accctcaagc
    14221 aaggtgaacc gttcgaggat ctcgccctgc gcttttccga cggcgcggat gcctcgcgcg
    14281 gcgggcggct cggcctggtt cgacaaggcg agttgattcc cgcgctggaa caggcgctga
    14341 catccgtgga cgtggggcag atcacgggca ttgtggagac ggcggaaggg ctgcacatcg
    14401 tgcgggtcga cgacaaaaag ccgcgtcagt ttcggccttt tgaacaggtg aaggccgaaa
    14461 ttcaatccct cgtgtttcag cagaagaccg aggatcagta ccagatctgg atggccgatc
    14521 tcaaaaacaa agcctacatc gagatcaagt tttaacagga tgctgaaaaa gtccgccagc
    14581 agcgttctcg cctcgttcag accctcaacg tacccccgag ggtacgcttc ggctcttcac
    14641 tcgctgcggc cttgctgttc ggcctttttg agcatcctgc gggagtgctc tcctgcgatg
    14701 tgacacatgc gtactatcaa agttcccgtg tgccacattc gtgtttccgc agtccattaa
    14761 actgcgtacg aatctcgccc cacaaggcgt cccgcccctc atgcgtttca gccgagtaca
    14821 gcacaatcgg cacctcgggc cccaatccgc aagcagttcg cacggcaacg aggctgggcg
    14881 cccgctcgct ttgccgcaat ttatcggctt tcgtcaggac gatgatcatg ccgcacccca
    14941 gagactggac ccactgtacc gtcatcacat cctgcggcat ccacacacgt gcctcgatca
    15001 acagaatcac agccccgagg gactgtcgct gttcgagata ctgctcgatc atcggccccc
    15061 attgcgcccg catggttttg gagaccttgg cgtaaccgta tccgggcagg tcgaccagcg
    15121 acagcaggga gagcttcgga tccgaggtgc ggaccgtaaa aaaattgacg gcgcgtgttt
    15181 tccccggcgt cttgctgacc ttggccagct tcttccggtg aagcagggaa ttgatcaacg
    15241 aggacttccc gacattcgat cggccgacaa acgcgatttc cggcaatcgg tcgggaggaa
    15301 actgttctgg ggagaccgaa cttttgataa actcagcgct gagtaatttc atggtgatct
    15361 accgcgtacc gacgtgcggg atgtacgttc acctccgggg acaggggccg gaccggtgca
    15421 acacctcgta tcctcataga tagagtgtcg ggaatgagca agtcaactgg cagcctgttc
    15481 ggccgaatgc tgttgtgggc gaccatcctg gtcggtctcc ccgcagccgc cttggcgttg
    15541 tattggctgg tgacgctgcc ggacgtctcg cacctggcca aacatcaccc cacggaaacg
    15601 gcggtcatgg aagcccggcg ggctcaggcc aaggaacaag gccatcctct gcacatcaaa
    15661 ttctcctggg tccctctggc tcgaatcgcg cccgcccttc agcgcgccgt ggtggcggcc
    15721 gaagacgcct cgttcttcgc gcacgaagga ttcgattggg aaggcatcaa agatgcggcg
    15781 ctctacaacc tggaggtcgg cgaattcaag cgcggcggca gcaccatcac ccagcagctg
    15841 gcgaagaatc tgtacctctc gtctgaacgc tccctgctac ggaaggcccg cgaagccctc
    15901 atcacccgtt ccctggaaca tcatttgacg aaggaacgga ttttggaatt gtatttgaat
    15961 gtcgcggaat gggggcaggg cgtattcggc gcggaagcgg cagcgcggca ccatttcggg
    16021 aaatcggcca aggagctgac gatcgacgaa gcggcgttac tggccgcgat tcttccctcc
    16081 ccgcgccggt atgacccgat ccgccatacc gcctacttga atcgacggca gcaccacatc
    16141 gtccgatggc tggaacgggg caacggacgc agagccggct cactctcgac gaaccgacag
    16201 gcaccggagc cgcacgacct cgccccttct gaaaactgag ccataacttc accagcacgg
    16261 tccgcacgat gctgcggaga gcattcgcta ctcagcatcc ccgactgccg ctacgtctcg
    16321 ttccccgaaa atcgccgact cgtgtcgata gtatttaaat tcgaggagat tccgggaggg
    16381 atcttggaga aagaaggtga gatgttcgat gcgggtaccg gcaaaccgcc gcctgggctg
    16441 ttggtaaaac gtcaggccct tcgctttggc gcgatcggcc agcgcctgcc attcttcttc
    16501 ctgcgtcaac accagcccga aatgacgcgg atagacgccc ttctgcggca ctgtcgactg
    16561 ggtggagaga tgggcgacca actggtgccc ggctagtccg aaggtgacgg cggaggtgga
    16621 ttcccgaccc agcacacaac cgagaccctc gacgtaaaat tgcttggctg cggcgagatc
    16681 gtggacagga aatgcgaggt gaaagagtgc cggcatacgc tgatacctct ggtcactgta
    16741 tcagcctctt gcgcgactag gcaaaccgac ctttcacgtg cgggcggccg tcgcgcccga
    16801 tcacaccggc caccatgttc ggcgtactgt gtcgaatggc gaaccggcca tccggcgaga
    16861 gcaccaacac cccggccgtg ccgtgaatac gctctacgac cttccgaagc acccgatttg
    16921 ccgaccgcag cggactcagg ccgctcgcca ataaatcgct gatctctttc gcaacggcca
    16981 ctcgaatgat gctctcccct aatcccgtca tggagaccgc tccggcctca ttatcggcat
    17041 agaccccgca tccgatcaac ggcgtatcgc cgacccggcc cggcaacatc acatccaccc
    17101 cgccggtcga tgcgccggca gccaccgttc cggcgagatc caaggccacc gcgcccacgg
    17161 tttccttgcc caagctgcgc gcacggaggc ggcccgtctg catgatcgcc tgatgcaagc
    17221 gcaacgtgtg cttcagcccg gcccgcggcc tcaagtcctt gggctgtggc cctgctgtcc
    17281 tcttggcacg gggggcgcgc tccaatccga aatagtccgc aaagcgggag gctgattgcc
    17341 cgaccagcaa cacatgggcc gttttttcca tcaccagccg cgcggcagtg atgggatgga
    17401 cgataccctc gatggaggcc acagcccctg cccgcagatc gcgcccttcc atcatcgaag
    17461 cgtccatgcg ccgcactcca tcgagctgac ggttcgaacc tcgccccgca ttgaacagtc
    17521 ctgactcctc cagcaggcga atggcttctt ccacgaccga cagcgccggg gttccggcca
    17581 tgagcagtcg gtggcattcc gtcagggcgg cctcgacgca ggcggcttga gcgacggtca
    17641 tggtgcggcg gcccgcgcca ccgtgaatga gcaggatcgg tcgtgatggt ttcaattcag
    17701 caggagatct ttcacgcggc ggcaggcagg atcttgcata cgatcgaggt ccgaccggac
    17761 gaaccccaaa ccggtcacac acagctcgaa attttccgac agcttccgga acaacgggga
    17821 atcctgctca gggtcgtagc tgccgtactc ggcgaccaac ccgtacgagc gtttgcccgt
    17881 cttcacgtaa tccaccagaa aatctttatg gtagatgcgg cccacgctct tgaggcggcg
    17941 gagatattct ggaaacaggc cggtcatgaa gagtgtaaag tcgccgatat ggcggtgcac
    18001 ttcgcgctcc cgctcgaagg attgcgcttc caacagcact tcggattcga acagcatctc
    18061 gacgacggaa tcgacggcgc ggccctgtgt attctggatc ttgtagagtt ggtcggtgtg
    18121 agcgaattcg acgagcaggt tggagacgta ctcggtgaca ttcagatcgg gccacccgag
    18181 gtgttcggtg aagctttttt ccgtcaacgc tccgaagagc tgtcggagtg gatggctgcg
    18241 agaaacggga cttcccatct cgacctcccg ggcaatccct gccctttgaa aatagtatag
    18301 cagaccatgc tcaacccaag cagactttct ccctgattcc ttatcggcat ccgcgatcaa
    18361 gacttgagag tcggagccga tgctcctcgc cggctgtcca acaggaacgt cacagggcca
    18421 tcattcagca attccacttg catgtgcgcc ccgaagagcc cggtttccac cgtcagaccc
    18481 ttcgcccgca gccgaccgac cgcctgctcg tacaatgtac gcgcctcctc cggtagggca
    18541 gctaggtcga accctggccg ccggcccttg ctggtatcgc cgaggagcgt aaactgcgag
    18601 acgagcaaca gcgccccacc ggcctcgatg agcgtacgat tcatcttccc ctgctcgtcg
    18661 ccaaaaatcc gcagggtctg aagcttctca acgatgtaca gcaggtcgcg ctcgtcatcc
    18721 cccttcgcga cacccaacag caccaggaga cctgcgccga tccggccaat gatccggccc
    18781 tccacttcaa ctgacgcgcg tgtgacccgt tgaatgacaa ccttcataca tcatccttca
    18841 cagaccgaca accagccaca atcactgagc atttcgcagt tgattcaacg tgccttccaa
    18901 actcaacagg cggcgcttca acggcaatcc gcaagaaaat ccacctagcg aaccgtcgtg
    18961 cgagatgatc cggtgacagg gcaccacgat ggggaccggg ttggccccga gcgccatccc
    19021 caccgccctg gcatactgct ttccacccac acgcatggcc acccattgat aggaccgcac
    19081 ccggccatag ggaatccgtg tgatcgcctt ccagactttc cgctgaaacg gcgtgccggc
    19141 cgaagaatcc accggaaaag aaaactcgcg acgcgtgccc gcaatatagg ccacaagctg
    19201 cgcgcgcgcc tcttccacga tcgatgcggc tgcgccgccg gcctcagccg gttgctgccg
    19261 ggcagcagga tccgacggcg acaaatcaat agaggtcacg cccttctcgg aagccgtaat
    19321 cctgatcaag ccccaagcgg tctgaaacgt gattgcgtgc gtcatgtcag gccgccccaa
    19381 acttcttcgc cactcgtccc aatttccgct tcaccattcc taacaccacg tccaattcct
    19441 ccgagccgag cataatatgc accccgagat atccgatgac gctgactccg attccaccaa
    19501 acaaggctgc ggatttcacg acccacgcac cgtcctggtc ccacagggac gcattcgcga
    19561 cccagaggca catcactgcg accggaatgc aggcccccag cactcgaaaa gccgaccata
    19621 caatcgaacc ccaatcgaca cgccccagac gccggtgcaa cacggcgacc aggatactcc
    19681 cattcagcat ggaagccagt gccgtggcca gagccagccc cgcggcatcc agcagcgtca
    19741 tcaaccacaa ggacaacaga atattcgccc cgaccgcaat ccccgcagtg accgccggcg
    19801 tcctcgtatc ctgtagggaa taaaacgcgg agacgatgat acgcactccg gcaaaggccc
    19861 acaacccgac ggcataacag aggagcgccg tcgccgtcgc caatgtatcg gctttggtga
    19921 acgacccatg ctcgaacacc agatgcacga tcgggtgccg caagaggatc agacccaaca
    19981 tcgccggaag aatgatgaaa aaaatcattc gcaggccgaa cccgatcgtt gtccgcaatt
    20041 catcaagcgc cccacgggcg gcctgcgcgg agagcgtcgg aagaatcgcc gtcgcgagcg
    20101 ccacgccgaa aatgccgagc ggaaactgaa tgagccgcat gccgtaaaac aaataggtcg
    20161 ggccaccggc gaaatacgag gccaggattg tactcacggt gatattgatc tgcgtcaccg
    20221 acaaccccag caacgatggc accatcaacc acccgatgcg cttcacaccc ggatgaccgg
    20281 gctgaaaccg caggccgaac aacatcccgc ggccttttaa acccggcagc tgcatcgcaa
    20341 attgcgccac ccctccgacg acgatgccga tcgccacgcc gatgatgggc tccggcaacc
    20401 agggcgagag gaacatcatg caggcaatgg tgaaaatatt gaaaaaaacc ggcgagaaag
    20461 ccggcgccgc aaaggcgcgc agcgagttca gaatccccat cgccagggct gccaggctga
    20521 taaagatcag atagggaaac atcatctgcg tcaacagcgt cgtcagcgcc agcttgtccg
    20581 ggctcccgcg aaagcccggc gcaaggagcc agacaatccc gggcgcggcc agaatcccca
    20641 gcagagtcac tgcggtgacg atggtgagca gcgtcgtgaa ggtggcgctg gccagttccc
    20701 aggcatcccg cttggtcttc agggtgtgat attcggtaaa gaccggaatg aaggcggcgg
    20761 acatcgatcc ttccgcaaac agttcccgca ataaattggg gatgcggtat gcgacaaaga
    20821 acgcgtcggc ggcgggggtc gcgccaaaga gtctcgctaa caccatgtcg cggacaaaac
    20881 cgaggatgcg gctggaaaag gtcgctacgc cgatgaggcc cgccgccttg acgacggaac
    20941 gggtttcatc cggagctggt cgagatggtt caacggaggc ggcgggatcc gacattatcg
    21001 gcgattgtag cgaaacgaag aggggcagtc tatcgttcac ggaaatcttg aacgtcgccc
    21061 ccatttggag catgccaaga gcatcatcag gccgctcgaa agaaatagcc ggcgcgtcat
    21121 tggcgtccgt agggccgcca gcagacctcc ggctatcgtc acaggcctca gaaacacatc
    21181 agctatagca tggctcgctg actcggccgg gcccactgcc tcctggccgg ataagtgttt
    21241 cagcacattg tgcgtgatgc gaatcgtgcg cggttcgcgt gccttgacca cgactcggac
    21301 ccaatcagtc gtggcgccgt tgaatacggt tcctccatcg gtaaaaacac ccatggttgc
    21361 aacgcgcagt cccggattgg ttgcttcgtt tgattcttgc caccaacctg tcatcccatt
    21421 gccaaactta ctcaactgcg cccatccgag aatttgaaag gtggctggtg tgccgtcctg
    21481 aaaagacggc acaagaggat gagcagtaga ggggggttcg gaaactgcag ctccgtcaca
    21541 ttcaaatcca acgagggcct gctctcgccc gaatgtctca ccgtcccgca ttccagtccc
    21601 ttcgtagacc caggaactgg agtgctgcac cgtaaacccc agagcttccc tctcggcccc
    21661 gtcccaccat gcaccgccat tacgatagct gactcccgtc acgctatttt ccactcgatg
    21721 tgccccagac caccaaagat ctccgttgtc gatcttacga cattccagct gggccccatc
    21781 caaacggact cggacccgcc accaacaggt attcccgcta aagagcgcca cgtttccgcc
    21841 agcatgaacg aacgattcca cacggtcccg catctcagtc gaccaatact cgtgatgccc
    21901 tgcgcacagc agaagacgat acgatgagag tgcggaggtg ccgtcatgaa ggtcgaggtc
    21961 cgtgcagaag tcgagttggt acccactggc caggagccag cctatgaagg gtgcgtccca
    22021 gtgggcaaac gtattccgcg gcgagctggc atcgtaccaa tcacaggcac tgccgcagta
    22081 gccgtcgtat tctccaccga tgtccccccc cacgcctgcg ccgggtcgac ggagggacac
    22141 gcgatgggtc tggtcaggac cgggcggatt gcctctcata tagaagttcg agcggccggt
    22201 atagttgtag gcttggtatg tgaatagcgg aagattgtac aggatgctcg cgtgcttgcc
    22261 aggcgcggaa ttcttcacca cgaacaatac gcgcccatgc cgaccatcaa tcgccggcgc
    22321 gctttccggc ggatccagca accggccctt cacatcttcg atcaacagag cgatatagac
    22381 tcccgatggc cattcgctgg gaatcgggaa ccagtattcg ggccagttcc aatctcgcgt
    22441 ccgctccgca gcggtagccg gttcagcggc ctgaataccc cgcaaccact cggactgcgc
    22501 gatatactcc agattggcac cttgccgata aaacctcact cgaaattgcg ggctggtcgt
    22561 agagacatgc aggccgaggc ggtttcccgg caacaaactc ggctgggcag cgtatcctcg
    22621 aatcatctca ctcgcctctg tcggcatgcc gagagctggg ctggtggcca tttcgaattt
    22681 cagagctacc cagttttttg tagtatgcgc gccccatcgc gcgtttcaag tggatttcct
    22741 taacgcccat gattttcctt ctactagccc cttggcctca ccctggcacc taaattgtgg
    22801 tcggctcgcg acggggtccg gcacccgttt cacaaaaccc ggctgcccgt ctctgcgagc
    22861 cggctggcgc aggacatgaa attgagcagc cgttacatct ttcatgacgg ccggcgcggg
    22921 atggtgccgc gcaagaacgt cttcgtgcct caggccaaga cgatcaactc tattcccatt
    22981 ttcaagctgc gcgcatcatc ggtacggacg acctgcgtgc gtttcccatt tgcattacgt
    23041 cggtaaacat ggcctgggta cggcccgatc ggacattatt ttgaattgta tcgaagacag
    23101 gtgaagactt ccattgtcca gcaaggacca acgatcaggg gtcttgaatc acctgattcg
    23161 acagctcatc gggattgtcg ccgtcacgcg caggacaggc gcgcgcaagg agcgttccgc
    23221 tggcttcgat gccgcagcag aggccgccga ccagatcacc gaccttcagt cgttcgacga
    23281 cgacggcgac gacctccttc cactgcgcat ccgagatccc atcgcgcaac gattggtctg
    23341 ccaacacaga gacctgccgt tcgagtaggg agatcagcag cagaagtcct gttcgctcac
    23401 gagtctgcgc gagaccatgc tgagcaaacg ctaatttcgc ccgcaactgc accttccgat
    23461 gcatccgctc gcgggatgtg aagagtcgca gcaccggagg ccatgtcccg acccaggaac
    23521 cgagggcata ggccagcatg atcactccaa gcaaccagac ggcattcgcc gcatgccacc
    23581 cccatgggag ccaggtggtt tcgatcgtca gcagggccgt cagggcgagt accgcgctca
    23641 gtagcccggc acgatggtgc gcctcacgat acaaaccgga gcgcccgacg atcatcggca
    23701 caatttcagc gctggtccgt tgctcggccg cctgcaccgc cgcctcaacc cgcgcacgct
    23761 cttcatctgt cagcgtcggc cgtctaccag tcgccactgg cgcctcctcc tccgaaatcg
    23821 cctccgcccc cggagaagcc accggatcct ccgaacgacc ccccgaaacc accggacccc
    23881 catccgcctt gttgcgtgct gaaccaggtc ccgtcaaacg cactgccgtc tccccgcctg
    23941 cgtgatccgc tgcttggccc cagcttactc agcaggccga ccagaaccag gctcacggcc
    24001 ccggccatca ctgccggtat cacccatggg gcagccagaa acgaaaggcc tcccccgaca
    24061 acaggcccca tcactcgatg agcgcgtgag aagaagagac caaccacgac tcccagaatc
    24121 accgcgaagg ccgcagtggc gaaggcatcg ctcgaagaag gagtagccgt cggatgttcc
    24181 gatgcacggt aggtcccttc aatggtttgc aagatggctt gcacaccggc cgtcacgccg
    24241 gccgggacat ctccggctcg aaaccttggt acaatctcgt tccggataat ctgagccgac
    24301 cgtgcatccg tcagcacccc ttccaatccg taaccgacct ctatgcgaat ttttcgatcc
    24361 ttgatcgaga cgagaaggag gactccgttg tcggtcccct tgcggcccaa cttccacgcc
    24421 gtcgcgacac gatgggaaaa atcaaacagc ggctcacctt ccaacgatgg aatgatgagg
    24481 accgccacct gattggacgt gttggcttcg tgcgccgcca gatcggctga gagtcgctct
    24541 gcaacggcag gcggcatggc atgcgcctga tccacgatgc ggccgctgag gagaggcaca
    24601 tccagcgccg cagctgggag gctcgccacc cctgtgagcc agagccaccc gatgaggagc
    24661 ccccaccccg ttcgccccat cagccgcatt gcaatcgctc cctgactcac gagaccgtcg
    24721 ttagaatttt acttcgggtg gcttggccac cgccttttcg tcagccacgg tgaaattggc
    24781 cttctcctcc agatgcagga gaaacttggc ggtgagattg gtggggaaat agcgaaccat
    24841 cttgttgaat tctgcaacct tgtcgatgta ccgcttgcgt gccacggtga tgcgattctc
    24901 agtaccttcg agttggctct gaagatcgcg gaacccttga tccgccttga ggttgggata
    24961 attctcggcc aaggcaagca accgccccag cgcactcgag agaccctgtt gagcctcttg
    25021 aaacttctta aaggcggcct cgtccttgag caactccggc gtcacttgaa cgctggtagc
    25081 cttggcacgc gcctgcacga cgctttccag cgtctctttc tcatgcgcgg catatccctt
    25141 caccgtattc accagattcg gaatcaaatc ggcccgccgc tgatattggt tgatcacttc
    25201 actccaagcc gccttggtat cctcatcaag accttgcaag tcgttatagc cacagcctgg
    25261 cagagccagc acggccgcaa gcagcacgac tccccccatc gaacgcgtca atgacatgaa
    25321 catgctctcc tccttcgcgc cggtcgtagc ggaccacgca tcccatagaa cgtaaggctt
    25381 tcaccggcga agtcaatcgc tggcgggacc cgcgagatgt cgattccttt ataaccttgc
    25441 tatacttgcc catacttagt ggatcagcac acaacgggaa gcggcatggc gctcgatcgt
    25501 tcgaccgata ctccgggggg ttttcaagtc cggcatcgag ccttgggggt gtttcaaggc
    25561 agctccatcg ggctggcctt ttggcatcca tcttctcata tgccggagta tgggctctgc
    25621 cgttttgcca ctgaggcaaa ggcacaagac tatgtcgatt ttctctcctc accggcatgc
    25681 cccgaaccat tgaaccgagc agacctgatc gtggaaccct tcgaccacat cgaacacgac
    25741 cgccttacga ctgagtatcc ccaggcctcc gcctgggaaa ctccactcta acagaacatt
    25801 gaagagagcg ctgtttcgct cacgtccgag cagactttgc tcatgattgg gcggctctag
    25861 gcaaactgga gcggagcacc tgaggaaata ggcgaggttg atacagctag ctggcggaac
    25921 ctgctccgga agattcagtc ttggcatgca caaacgcaag aagtcgcttg ttttcttgcg
    25981 ccgaccgaag aaatttgaac gcaataccgc gggaactcac agaccggacc atcgcagcca
    26041 cttcgagcgg cgtctggtcg ttctcaagcg cgatctgaag ataaaagata tcgtccatat
    26101 gcaccggagc ttggctctca atgatgcacc cgcccatcga aatgtccaag acggttcctt
    26161 cgcccctgac tttgccgcca gagaaggaca aaaacaggcg cacgggaata cgcagatgtt
    26221 ttcgccgatc gagcatctgc gccggataac ggcgtcccaa tcggaaggcc tggaaacgga
    26281 agctacaaga ctgacaatgg aacggggcaa tacaagcaag cgactgcaac cgctccatca
    26341 gcgtggagca gcgtgaacga aacacattgt cttggccgca ctgcggacac gtgaggggtt
    26401 ggatcatgct gaatagatcg gcggatgtta gcgccgctct cccgaataca agatactctg
    26461 ccggacgccg gtcacggctc cacctgaata ctcgccacag atggccttga ggcaaaaccg
    26521 accgcggaca cagatcacag caacttccca cagtctgtac accaccggcc gtaggcgcag
    26581 cggaatggcg ctgtcagttc aatcgataaa ggatgggggg agttctgtca agaaactcaa
    26641 cggttcgctc cagaaggaag gcagattcac tgattagccc ttctcttttc agaggccagt
    26701 cagcgtagat ctgccgattt cggctctcgc cgaattgacc ctgtgactcg tcatgttccg
    26761 agcgtgcatc gccgccacaa ctctcatcgc acacctcatt ttgtacagaa cgcttcccct
    26821 tcgctccgaa tttgtccaac cgtgaaatgt gaacgccctg atatttcgac acaatcagga
    26881 ttaaatggtt ttaacgtctc actaacacct ctttaatctc gcgttgctac tctctcctca
    26941 catgggcacg gcctttagat aataagggcg caatcctaag gaggtgggaa ccatgacatg
    27001 cggacgatgc aaagggttaa tggtcgatga gtggaggccg gacttttcgc cggaaacgtt
    27061 tgtgtggcgt tgcattaatt gcggatcgat tgtcgatcca ctgattgaac aaaaccgtcg
    27121 aactcggctc gctgaacaac tgttattgga gccagtcgca gtattgcagt aatcggggag
    27181 tcgcaggacc gtcctgacaa acgccccggt cctgcggcgg tggtggacga gtagaattgt
    27241 cgctgcggcc ttcaaaccgg ccgtagcgac gatgaagata gagagagaac tgattaccta
    27301 ctcagtaggt tcgacgtccg aacgaggcga gggccgatcc ggcaagcctg ttcaatgcat
    27361 tgctaggtgg agaactcggc ccagaggaca gtgtgatcag aaggatcggt cgcacggcgt
    27421 ggctctaaat cgacatcaac gagctgacac ctctgagcca agggcggcgt tgccagaata
    27481 tggtcgatcc gccatccttt attggcggca agggcactgg gtgcacggta gtcaaaaaac
    27541 gtgaactgct ggcgatctgg atacagtctg cagaacacat cttcgaagcc ccacgcaacc
    27601 gtcttcccat aggcattgcg cgcgtcttcg tggtagcaga catgtttcag atgtttctcc
    27661 gggctgtgga catcaatggg tcttggggcc acattcatgt ctccacacca gatcgccggc
    27721 tccttcgggg agagatgagt ttcgaaatgc ttccgtaacc gatcgtacca acccagtttg
    27781 tattggtact tgggcgaatc gatctcgaaa ccctgcggaa catacgtgtt gatgatcggg
    27841 atgccctgaa tcaccactcg cagaagacgt gcatcttccg tctcgccccc gtcatcgaat
    27901 ccatacgaga ttgcttccgg cttcgtgcgg ctgagaatcg caacaccatt gtaagctttc
    27961 atgccccgga aggtgatctc ataaccggag gtcgcgagcg cagtcagagg aaattcgctg
    28021 tcctgaactt ttgtttcctg caaacagagc acatcaggct gatggcgttc gagccactgc
    28081 agcacgatag ggaggcgctt gcgcagcgag ttgacgttga aggtggcgac tttcatacgg
    28141 cggggattct agcagagccc acccgccgca acaactgaag catacagaac aacaaggcct
    28201 gtgatcggac atttcccgat cacaggccgc aattgtcgaa tatcagcgtt atccagctct
    28261 cagccaaccg gccgacctta gttccgcatc gcccccgtca cgtcgttctt catcccgccg
    28321 gcctcgtgag ccgcatcgtc ggcaacactc ctcgcttctg ccgccttcgc gcgcgccgcg
    28381 tctttcttgg ccttgacctt ggccttcatg tgctccttct tcgccttcaa gtcggccttc
    28441 gcttgctttt tcttcgcttt cgcctcgtcc cgcttcgcct tcatctcgct cttcaccgat
    28501 tctttcttgg ctttcaggtc actcgccaag tcttccttct gagcgttcag gtcgttcgac
    28561 agtccgctca tgtcctgctt catcccctcg gacatcttac ccagccccat atcatcggcc
    28621 acagcgaatg aactcattcc cacaaatgcg acggcgacca acacagcaat tgaccttctc
    28681 atcaacaact cccccttggt tgaaatatgg tgtccggctc tatggcgaac cggccgtcat
    28741 gcaggactca tcaataccgt gaaggggcga gtttgccaat tgttttgcca gtttttgccg
    28801 agaccgcgaa tccaagcagg acaagccggc ggcagaggct tcacaaaatt cggcaagaaa
    28861 ctggaaggat cgtcgcaaga ccttacggcc gcacggcgat gagatagcgc ctgagaatgg
    28921 cttgttcgac ccgtgaacga ccacggtcgg gggccggaaa cctgagcacc agatgaatac
    28981 gtcctgcctc agggagttgg atggtaatac gtggctcagg agacggcgct tccagcaggt
    29041 ttcgctgttc cagcaatttc atctgtcggc ccatttgatc catgaaggag gcgcactcga
    29101 cttttgcggc attgagcaga gcttgctcag cggcttgcca atcgtccgtg ctctgaagcg
    29161 gaaccgacaa aacgtagagg ccgtattcct gcgacgggct ctctttgatc aagggatttc
    29221 caaacagaag actgttggga aagaccacgg tgcgaccggt gtagagatgc gatgtctgac
    29281 ccggcccgat ttccaacagc ttcgtcgcaa agacgtcgta ttccaggaca acgccccggt
    29341 aggtgcccag ctggatacga tcacccacac cgtacacccc cccgccgaca cgcaaagccg
    29401 ccccgctcca gcagagaatc aattcctttg tcgccagcac tacggtagcc gccagcgcga
    29461 ccagagacac agcaaacgct tccaactcgt gggcccagat cacaacgagc cccacaaaga
    29521 gccccagcac catgctgttg cgcgccgcca cgatccatcg tcgcttcgcg tcgatggtca
    29581 acgtgtgatt gcctttgatc caacggacca gaaccgtgcg tgcgatgagt agaatgagga
    29641 gaaggaggat ggacttcaga aggtcgaaga agacgctcga tccgatcacc ggcaacagtt
    29701 cgcgcataat gtcgtgtgtc cgccttattg ggatgcggtc tcatcctcac tggtgcgagg
    29761 aacacattct tcccgtttcc aatctttcag caacttggcc tcatcaaacg acagcgtata
    29821 gcgataacaa tagagcggct cccggtacgg ttcgtcatta ccggccaggc tctttccgaa
    29881 cggcgagggc gagggcgggg ctttggtttt cttgccggcg gtcaggctac cgggatccca
    29941 gggatgcact tctcgatccc ccatcggcac gcgataggtc cagacggtgt caccacccag
    30001 caccggggtc ttagccgtac tcggaggacc gaatttttct gcaatatcgt cctgcgtgag
    30061 cttcttcacc cccttgtcca agtaccgatc acgccaaggc ccgccgcaac cggacaacat
    30121 tgcgacacac agtaggagca ggagggcact ccagcgaccc gggcacacca ttgtctgctt
    30181 cgtctcgcgt ctcaactggc taccgttccg aatcacgatg ttcccgaacc caccggattg
    30241 aacaatacat cgcatagccc tgcgccatca tcccggtgac gagcagcccc aacgcagttt
    30301 gaagccacat cgtgttggga aattccaagc cgtaaatacc aagcataaag ccgccggcaa
    30361 tgaagacaat acccatgccg cgcgcaatca atgcattggt ccgtaaatca gacatccttc
    30421 gccctcacgc ccttgcgaat ctgtacgcta caccggcgca tcccatcggt tcaactctgc
    30481 gccccccgcc atcacgattt cggttttgcc cgaatcgcgc ccgccctccg ctcgagcccc
    30541 tctgcctctc cgacccgccc cagcttgcgc agcgcactcg catagtcctc aagatttttg
    30601 gccacatccg gatggtcggc ccccaactgc ttctcccgca ccgccaaggc ccgcgcaaat
    30661 aacgcccctg cttgctcccc gtcgccttga gccagataca tcgcggccaa attgctcagg
    30721 gccagcgcca cctccggatg gtccgttccc aggagcttct ctttgatcga gagcgcccgc
    30781 gtcaggaggc gctgcgcatc ggcatattgt ccgtgcttcc gatgcaaaac accaaggtta
    30841 ttcaacatgg ccgccacatc gagatggttc tcaccgtgca catcctggta aatcttgagc
    30901 gcctctagat acaccggttc agcctcgacg aatttcccct gcgcgctata ggcttgggcc
    30961 aaatgggcca aggtcaccgc gacccgccga tcatgtaggc cgaacttttc tgccttatgc
    31021 acggcggcag agaaaatttt ttccgcctgc tcatactgcc ctcgctgcaa tgccgtttca
    31081 cctgcctgca tcgcagcatc ccacgtctgc tgcccgcagg ccagagtcac cagcagcaga
    31141 agcgccgatg cacccgcggc aacggttcgt ttcatgctcg acccatctgc tcgacaatgg
    31201 actccgccgg atacgtcacg atctccgcga gcgtcggatg atagtgggga atgcgcagca
    31261 ggtcctgtgc ggtgccgcga aagtacatca ccgcgatcaa ctcatggatc aattcccccg
    31321 cctccggccc cacaatctgg gcgcccagca gtgcgccgtc gtccggtcga cagagcagct
    31381 tcacaaatcc atgcactgcg cccagacaca tggccttccc atggtcggca aacggatagg
    31441 acgccgttag ataaggaatc gcctcttcgc ggcactgccg ttcactcatt cccgcaaccg
    31501 caacctgagg ttcggtgaac acgacttcgg catccaaccg gtgatcgatc acccgcgcag
    31561 gctcattcgg atgcgtcgca ttaaacccag ccgtttcgcc ttgttcaatc gccagatgca
    31621 caatgggagt gagatcgttg acgtcaccca cagcaaagat gtgcggttga gaagtccgca
    31681 tatccccgcc gaccaccagc cggccatcga ccacgcatac tccggcggcc ttcagattga
    31741 gccctttcag gttaggacga cgaccgaggg cttgaaagat aacctcggcc gatcgcgtat
    31801 ggacagcccc gccctgcaca aagtgcacgg tcttctcaga cggggttgag ctcacacgtt
    31861 ggaacgacac gccggtcatc acctcgatcc cctcatcctg aaaggccgcc gccagcgcct
    31921 gactgatgtc ttcatccatg tcggacaaca aggttgtgct acgctgcagc atggtaacct
    31981 ttgtgccgat tcgggcaaaa aattgagcga actcgacggc gaccaggccg cctcccaaca
    32041 ccaggagcga cgccggttgg cgacgaatat cgagcatctc atcgctggtc agataaccgg
    32101 cttcgtccag ccccggaatg gccacctcag ccgcactcga cccggtggca atgacgaatg
    32161 cgccgccgcg aatccgaaca tctccggcac gaagttcgtg gggtgagaga aactcggcgc
    32221 gggactcata caacgtgaag cgcgggtctc gcagtgcggc gatacggtag tcggcgaact
    32281 cttgaactaa tcggttcttg cgatcgacga tagccgacag atctgcttgg gcagtgaccg
    32341 gactcagccc gaactcgcgc gcgcgcttca tcagcgcgat gatttcagcc gaccgcagaa
    32401 tcgttttggt cggcatgcaa ccccgcagga tgcacagccc cccgagcgga ccctgatcca
    32461 cgatggccac atcggcgccc gcgtcgcgag ccgtccgtgc ggcggcatag ccggccgatc
    32521 cgccaccgat caccacgaca tcgtgccgcc gttcaggcat gtgagagagt tgtgatgaca
    32581 ttcccggtcc gatagactgt cccgcaggca tgaacccgca aggatacact atgatgaaac
    32641 ctggtcgcta tcgtcactac aaagggaacg actatgaggt gctgggcgtg gctcggcatt
    32701 ccgagaccga ggaggagtat gtcgtctatc ggcaactcta tggagcaggc gggctgtgga
    32761 tcagaccgct gggaatgttt ctcgaatcgg tgaccatcgg cgagaccgtc gtccctcgat
    32821 tccgacggtt agaggaaggt ccagcgtgag aaggtcgcgg cctgtccctg acgacgcccg
    32881 gcttggtcga tcacgctcca cacaatcgcg ctttcgacca ggaaccgtcg tccgctgttc
    32941 gaaattctga ccccgcggta attatccaca tacccctgca cgcgagcccg ttcaagcatc
    33001 cactcccgct cggcacgatt cacagcttca gccgtcaggc gggatggagt ctgcaggaac
    33061 tgctcccacg tcgtctccca caaggtcagc gcgagatgcg atccgtaatt caggatcgga
    33121 tcctcctgcg caccgtgcga gaccaccaca aaaggcgcca tgaacaatgc atgggcctgc
    33181 tcctcgggcc ctccggtcct gagcatcaag tcccgcccga cccagtggcg ataactgttc
    33241 aatagcagct gggaccattc gacgaccgat ggctgactcc acacttgatt cggatcgtcc
    33301 atggcctcct cggcggaata ggtcgatgca ttgtgcccgg cgcatgactg acttgccggt
    33361 agtagcacgc ctcctcactc catgccaagc aggttatgcg gcgcgctttc ttaaatgacg
    33421 gacaaacaac ccctgcccgt tgctgcctat caaggtcatg accagatcac cgcgttgcag
    33481 cctggttcca tctcccatcg cctcaggatc ttgggtgatg accctgcagg tccaggtttc
    33541 caccgtctca gtgtgtcccc tcgtcttgag cacgacgcgg ccttttcctt caactcgata
    33601 ggtcatgtcc aacggcaccg cctcaaacag ctggtccgga tcgccggaga ggggctggag
    33661 cgagtgggcc gatttggctc cgccgcgatg cgagtattga aaggcttgat gaggaaacag
    33721 atatggagcc tgtgggtttc tgaaccgata cgaggagtcg aaggcaacca ggtgccaatt
    33781 tccgggcaga tcgtcgcccg cacaggtccg tgaagagtgt ccgtgagtgg caagtcctgt
    33841 tcgcatcagc gactttgccg cagggtggtc cttcgcctcg gccggacacc aaccggccac
    33901 acccagacag cccgctacca cgaacgctcc aagccatcgt gccatacgat cctccgtttc
    33961 agaaatgatc tctccggccc catgcccgga gcctgcccga agacaagcct ggcagggatc
    34021 gaagattgtc agacactcag cgagattagg gttctttccg tacgtctgct gccgtggacg
    34081 gggcgggaag cgctttggga gcgcgcggca gcacacgata ctcgatcaac cggcaagtac
    34141 agaacgaaac ctgccgccat tcggcggtaa ttttcacgat cgccgatcca cagatcgtgc
    34201 aacccagcgc ccggggatcg cgtttgatcg tccagcgggg aatcgtgatg cgttcggcgt
    34261 cgaccggttc ctgttccatg ccaaccttat cggtcctcgg ttttaaaatc taaagaggca
    34321 atttcttccg gccccccttt gcccttgatg ccataggcct gctcgcatat ataataggca
    34381 cgcatcgcgc ctgcgccttc aatcacttgc aacggcgcgg gtctacaagg agtatcgcca
    34441 tggctgcaca gacgttattc gaaaaaatct ggaacgacca cgtggttcgg gcggaaccgg
    34501 acgggaccac attgctctat atcgaccgcc acttagtgca tgaagtgacc tcaccgcagg
    34561 ccttcgaagg gttggccgtg gcgggccgaa ctccgcgcag gccgggcgcc gcgctggccg
    34621 tccccgacca taacgtcccg acgaccgacc gccgcgtggc tatcgccgac ccgatcagcg
    34681 ccaaacaaat tcagacgttg gacgataatt gcaacacatt cggcatcacg atgttcggca
    34741 tgaacgacat tcggcagggc gtggtccatg tcatcggccc tgagcagggg ttcacgctgc
    34801 cgggcatgac gatcgtatgc ggcgattcgc acacctcgac ccacggagcc ttcggcgcac
    34861 tggcgttcgg catcggcacc agcgaagtcg aacatgtcct ggccacgcaa tgcctggttc
    34921 agaaacgccc caagaccatg gaagttcgtg tcgacgggca actgtcgccc cgctgctccg
    34981 ccaaggatgt cattctggcc atcatcggaa aaatcggcac ggccggcgga accgggtacg
    35041 tgattgagta caccgggtcg gccattcgcg cgctcagcat ggaaggccgt atgaccctct
    35101 gcaacatgtc gatcgagggt ggagcacgcg ccggcatggt ggcaccggac gacactacct
    35161 ttgcctatat caaaggccgc ccgatggctc ccaagggcgc cctctgggat caggccgtga
    35221 cggcctggcg gcaactcgcc accgatcccg gcgcgaaata cgatgccgtc gtagagttgc
    35281 gagccgagac gatcgccccg caagtcacct ggggcaccag cccggggatg gtcactggag
    35341 tggacgggaa cgttccggac cctcgcacca tgggggacga caaactccgc caggcgacgg
    35401 aacaagccct cgaatacatg gcgttgctgc ccggcatgcc gatccgcgac atcaagatcg
    35461 acaaggtatt catcggatcc tgtaccaatt ccaggattga agacctccgc ctggccgcct
    35521 ccttcgccaa ggggaaaaaa gtcgccagca ccgtgcatgc catggtggtt ccggggtcgg
    35581 ggctggtgaa acaacaggcg gaacaagaag gattggatcg tatcttcaag gagtcgggat
    35641 tcgaatggcg ggaagcgggc tgcagtatgt gcctggcgat gaacgccgac gttctgcagc
    35701 cgggggaacg atgcgcgtca accagtaacc ggaacttcga agggcgccag ggagccggcg
    35761 gacgaactca tctggtatcg ccggcaatgg ccgtggctgc cgccgtcgaa ggacatttcg
    35821 tcgatatccg tcactggtcg taagccagga cacattccgg aactgccagc ggcgcgattg
    35881 agtcggcgcc ggtcaggcac gaagccgaag aggacacacg aggacatata tggacgcatt
    35941 cacaacactc acaggactgg tcgctccgtt ggaccggctc aatgtcgata ccgatcaaat
    36001 cattccaaaa cagttcttga agaccatcaa gcggacgggc ctgcgcgaag gactgttcta
    36061 cgattggcgg cggctgaagg atggctcgca ggatcccgcc ttcttcctga atcaaccccg
    36121 ctatcacaac gccacgattc tgctggcgcg ggacaacttc ggctgcggat cgtcacgcga
    36181 acatgcgccc tgggcgctgc tcgatcaagg gtttcgttgc gtcctcgccc ccagcttcgc
    36241 ggacatcttc tacaacaact gctttcaaaa cggcattctc ccggtggtac tgacagggcc
    36301 agaaatccag gcgctattcg agggcgtgac ggcccgcgaa ggctatcagc tgacggtgga
    36361 tctggcggcg cagcaagtca ccacgccgga cggcgcgtcc taccatttcg agatcgatcc
    36421 gtttcgaaaa gattgtctgt atcgaggatt ggacgcgatc ggcctcacgc tccaacatgc
    36481 cggtcggatc acggaatacg aacaacgccg ccgcacacaa gccccctggt tgtttcaaga
    36541 cgtgcgctaa ccgtcgggaa aggaacgccc tatgacgtgg tgggaaacca ttctgtttcc
    36601 ggtagcctgg ctgtggagac cgaaacttct cttcgcgttg ctctttttcg gcggggccgc
    36661 ttatctgctg atcatcttca actggagtta ttccgacgga gaccgcgtgg gctatctcca
    36721 gaagctttct cggaaagggt ggatttgcaa gacccaggaa ggcgaactcg ccatgacgac
    36781 tgtgcccggc gtcgcaccgg tattatggag tttcagcgtg tgggatgatg ccgtggccaa
    36841 gaaactcgac gggcagatgg gaaaacgagt ggtcctccat tataaggaat tccgctatat
    36901 ccccaccacc tgcttcgggg aaaccgccta ctttgtggat cgcgccgaag tcttggattg
    36961 acggccccat tctgcacgcc atcacagcca cttcttttgc ttgaaaaaat acagcatggc
    37021 cagcccggtt gcggccatga cacccagcac ggccgggtat ccccagggcg attttaattc
    37081 cggcatgtgc tcgaaattca tcccgtagat gctggcgata aagctgagcg gcatgaaaat
    37141 agtcgtgatg atcgtcaaca ctttcatgac gccgttcaag cgatagctca cgctggagag
    37201 atacacttcc atcatggacg acaccatttc gcgcaaggtc tcgatcgtat cgccgatctg
    37261 aatgatgtgg tcatagacat cgcggaaaaa caccttcgtc gaaccctgca gaaattgacc
    37321 gtccgagcgc gacaggttgt tcatcacgtc ccggagcggc cagacggagc gacgcagaaa
    37381 cagcagttgc cgcttgaggc cgtggatatc gcgcaatgtc tccggaccag ggttcgtgac
    37441 gcacagatcc tggagcgctt caattctttc tcccagcgtc tccagaataa cgaaataccg
    37501 gtccacaatc gagtccatca gggcgtacaa gagataatcc gcgccggcat gacgtaaccg
    37561 gcctttcccg ttccgcagcc gttccttcac accttgaaag acatccccgc cgttctcttg
    37621 aaacgacagg agaaagttct caccgaatac caggctgacc tgctcgacgt tgatatcgcc
    37681 ctcccggtcc ccttcgtaca gcatcttgag caccacatag ccgtacgagc cgtagtcgtc
    37741 gagtttcgga cgttggtcgg tattcgcgat atcttccagc agcaagggat ggaggccgaa
    37801 cattttcccg aaactctcca gcacctccat cttgtggatg ccgccgacgt ccacccatcg
    37861 gaccgtcggc tcccccgtca tcgcgatctc gtcaggtttc gacaccgctc gctcttgaaa
    37921 ctgtccctcg ccgtattcgt acaccgagat cgtgaccgtc tcggactttt tttcgccgat
    37981 atggaccagc gtgccgggcg gcaatcccgc tttgcgggaa tgtttgtgct tcaacttcat
    38041 ggtcagtcca gcttgtacgc ggaaccggca ggagggtcaa gagcctggcg cggcatcaag
    38101 ccactccggg ctttgttccc gaaccaagac ctggtacggt gaagacatgc agccatcacc
    38161 cttctctgcg aaccgggagc ccctgtgacg atggatcatg aactcagcat cttgtcggaa
    38221 gccatgaggc gggccgggcg tgaagccctt cgaatggcgg ccaccggatt cgacacctac
    38281 accaagccgg atcagtcacc cgtcacctct gccgacctgg cggtcaatca gatccttcgg
    38341 gaccatttat ccgcggcatt tccggaggat ggctggttgt cggaggaaac cgaggacaac
    38401 cacaaccgcc tgaccagatc acgggtctgg atcgtcgatc cgatcgatgg gacgcgctcg
    38461 ttcgtgcgag gcctgccgga attctgcctc tcagtggcgc tcgttgagca gggcatgccg
    38521 gccgttgcag ccatcttcaa tcccgcgacc ggtgaattct tctcggccat tcgcggtcag
    38581 ggcatccgcg tcgagcgcca ggcagaagcc agccaggctc cgttcaccgc gccggagcgc
    38641 ccactcgcgc tcgtgaatcc atgggaactc cgggtcggcc gctttcaggc cctggagccc
    38701 catctccttt gccgtccgat cggatcgatt gcctacgccc tcgccctggt cgcagccgga
    38761 caggccgatg cagtcgtgac gctggaaggg ggcaatgagt gggacatcgc cgccggcgtc
    38821 ctgttgatcg aagaaagcgg agggcgcgcc accaccgccg caggccatcc gtttacgttc
    38881 aaccgcgccg atcccagggt gaagggcacc ttggccatcg gcacagacct gaccacaccg
    38941 acgcgcacac acctcattca actcagtgcc gcaagtagag accttcgcgc ccctctccct
    39001 tgaccctttc aaccgccgcc gcttctctgt acgatgtccg tctcgcgtag aggatggagg
    39061 ttacccatga acgtagcctt gttaggcact gggttgctgg ggcaggcagt ggcggagcgg
    39121 ctgcatgcca ccggccatca cctatcggtc tataaccgta ccactgacaa gaccctggcg
    39181 ctgcggcaac agggtattcg tatcgcctcg actgcggtag aagccatcgc cgcggcagag
    39241 gtggtcatcc tcttgctggc agacgcagcg gccattcgtg ccgtactgct tacgccctcc
    39301 gcccgcgagg cgcttcgcgg acgcaccatc atccaaatgg gcacgatcgg cccgtcggaa
    39361 agttgctcga tcggagaaga catcgagcgc ctcggcggac aatacctgga agcgccggtg
    39421 ctgggaagca tcgccgaagc caaagcgggc accctcctga tcatggtcgg ggcgactccg
    39481 gagcgttatg cgacatggtc gccgcttctt catgccctta gttcggacgt gcgcctaatc
    39541 ggcccggtgg gaaaagccgc cgtgatgaag ctcgcgctga atcagctcat tgccgcagaa
    39601 acggcagcgt ttgccctcag tctgggcctc attcgccgta gcgggatcga tgtggagaat
    39661 tttatgacgg tgttacgcaa gagtgcgctg tatgcaccga cgttcgataa gaagttgcct
    39721 cgactggcgg aacgcaacta tgcccggccg aacttttcga cccgccacct gctgaaagat
    39781 gtggagttga ccctcgccgc cgcagacagt gcccagctct ctcccggcgg acttccggga
    39841 gtccgatcac tgctcagcga cacgattgcc ctggggctgg gagatgtgga ctactcagcg
    39901 ctctatgaac gggtggaccc tgtcaccggc acgtgactct ggtctctact gggaggcgcg
    39961 cgcgccgggt acaggctggt tctcgacgat cgattgcacc ttcatcacgt ggaacgttcc
    40021 accggccttg gtcggcgcgg catcactctc gatgcgtgtg taccaccaac gaccttcgcc
    40081 ttccgagtag tctggcgaca gggtgatctc gaatccgcct tcccgatcgt tttcccgttg
    40141 agcccaggcc ccggcccaac ggtgattgga gaggacatcc gtccgaaacc gtccatcctt
    40201 aaagtcatac gagccgttgc cgaattgatc caatctcaac ggcacgacca tcccgctttc
    40261 ctcatattcc cattcgcctg caaggaccgc atgttcagcc cgatggaggg aaatttcccc
    40321 cgacacaggc tccgcccagg aagcctcctg tggccgtggc gcatgactac aggcctgcaa
    40381 caccagcgca ccgaagcaga tcgacatgaa cgaaagacgc ttcaacatca tcgtgtgaat
    40441 tcctagtgtt tccgtacgca ctcttcttct ggtgtttctt cgaaactatt tatttaccac
    40501 gcatacccgc ctgcctcaag catcatgtga attgtggctc atttcaacca gttgcaccaa
    40561 ttgtctcata ttgcgccacc agccggcggc gcaaccattc gaggccagcg ggtgagccca
    40621 cgggaacgtc acgcctcacc cggccgcctc ctatccaggc tgattcctca ccctggatcg
    40681 tctcaaccga cagggcgcca gtgtgtggaa attgatcggg ctgagtggtc gggaaaaagg
    40741 ccacgtgggg aacgatgacg aggaccgcgt cctggcagac gcgctaacgc tgaatgagtt
    40801 ccgaccgccg gcggccgtgt tacaccgcct gttcgctcag gacatgaatc gttcgatggc
    40861 ctgactggct cgctccagat ctgtgtctgt cttcgccata caaaatcgca taaaccgact
    40921 cccctccggc ccttcgaaga acgcttcgcc cggcacgccc gccacaccgg tcttatccaa
    40981 tagatacagc gcgcgttcgc gactcgtctt tcccggaagt cgggacacat ccgccagcac
    41041 gtaataggcg ccttgcggca cggcaggggg cagtcccgct ttggctaacg cgccgcagaa
    41101 ccgatcgcgc ttttgctgat gctccttcgt caggtcgctg taaaacgagg ggcccagtgc
    41161 gcgaatcccg gcggccacgc catgctgcag cggggtgggc gcgcagacat acaacacatc
    41221 actcatcgca ccgatggcct tcgcccacgt ggcctccgcc acgctgtagc cgatgcgcca
    41281 accggtaata ctgaacgttt tcgagtaacc cccgatggta atggtccggt cggccatacc
    41341 ggggagagac gccatgctca catgctcccg tccatcgaac acaaaatatt cataaatttc
    41401 gtccgtgatc accatcacgt catggcggca ggccatctcc gcaatctgtt ccaactcccg
    41461 ccgtgtgaag acttttccag aggggtttcc cggagtattc accacgatcg ccttggtcct
    41521 ggatgtgatc gcctgctcaa ggcctttgaa atccaccgtc cagtccggcg gtcgcatcga
    41581 cacacagcga ggcactgcct cgaccgcaac gatcgcctga atgtgatagg catagaaggg
    41641 ctcaaagaga atcacttcgt cgccgggatt cagcaacgcc atgcaggtgg cctgaaacga
    41701 tccggtggct ccggcgctca cggtaatatt gacgtccggg tccgccgtga ttcgattgta
    41761 cgcggcgagt ttgtcggcga tggcttgccg caattccaga atgccgtcaa agcgtgaata
    41821 gatgttgtgc ccttgtgcca tcgcctgttg cgcgccctgc accacgaccg gaggcacggg
    41881 ggtatcgcac acaccctgag acatattaat cccgttcacc ttggcacagg cgagggtcat
    41941 ggcacggata tccgattgag ccaaacgagc agtccgatcg cttttcttca tcactatcct
    42001 caaaaatcgt ggtctggttt ccaccggctt agcggccgga ggccgtctcg ccgcaattgc
    42061 gcgagtcaga gtccagtacc ccttcctagc ggaaaatgca acagacacag catgcctgca
    42121 gcggggcacc tgtcaacgca cagaccggct tcattcgcgc acccgagggg aagccaggaa
    42181 cgcctcgccg cctgattccc gcgtcctgga atgtgcagcc gtctgatggg actccgcacg
    42241 gaatcgatgg taatgactca gcacccgctc gacatagagc tgcgtcgcgg gaagcgcggg
    42301 ggtgccgagc gtctgcctga ctcgcgtctc accgacatga tacgcagcca gcgccaacga
    42361 aaggtccccg tcgaaccggt tcaagagccg gcgaagcagc gcggcacccg catagatatt
    42421 gtccttcgga tcgaacggat ccagcacatg gagcgccgcc gcggtcagtg gcatgagctg
    42481 catcaacccg atggcccctt tcgacgacac ggctcccgaa ttgaaattcg actcgacctt
    42541 gatcacagcc cgcaggaggg cggggtcgag cccgtattgc gcggcagagc gggtaatcag
    42601 cgcggtgatc gattcggacg cgcgaggaaa cggaccgtcg cgcatgccgg gatcgatcgc
    42661 gactcggctc accggaggca tcatcaccac caggcaccct gccaccacaa acgggcagag
    42721 cgcgcgggca acacgctccc aacgggaatt ccattccggc tcttgcatga tcgtgcgagg
    42781 actcgagagg cgtgggcctg gctcgtccta ccggggctcc tcatcaaggc ttctgatgag
    42841 cacggctcgg ccctgccgat cgaaccagga ttggatgcga tcgcccacat gggccggtcg
    42901 atcgatccgc gtattttgat catgggggat acgatattcc tgtccgcgaa catcgcggac
    42961 cagatacgct ccgccttcca cggcttggac ctgaccgacc aacgacccgc cgggctggtc
    43021 accggccgtg gctgcaaccg gaatttgcgc accaagcgtt cccgtcacga tgcccatgcc
    43081 aaggttttct tgcggggagg gtgcctgcag ggctgactgt gtacagccca tcacaaagaa
    43141 tcccatggga aggacgaaca acaaacgacc gcacaccgtg agcaagggca tcatggctgt
    43201 gacctccagg gatggttggt tgggaagccg gtgcaagaaa ggcctgcgca ccgtcatgtc
    43261 actattccat ggaggggcgg aatgctcaag aaaatgacac aaatcagctc agagggaaat
    43321 ggatggaagg tgcgggaaca aggttaccgg gaaacgggac gcgacacagt gatgtgacgt
    43381 tggcgatgcc acaggtagaa cgaatagagc gtcgccgtca gcagaacgcc catcagcaac
    43441 atgagacaca gcaccgcgag ggcgccagcc atggcattgt ggtaggtccc gtaaatcacg
    43501 tgcaccgtct ggtcggtgaa gggactgaag tgggacaacc atccaaagac caatcctccg
    43561 catccgatga tggcccagaa caggggcccg acaacttctc tacgggatag ccgtactccc
    43621 actccaatca cgagatagat ggcggcaacg acgagcacaa caccaaccga ttgcccgtca
    43681 agcggccagt ggaagtctcc ccgcatgatg tggtcgatcg tatgcacccc attcagccag
    43741 gcaataacca ggatcaggcg gagcaggtaa cgatccacag tcatgcgttc gccggcctga
    43801 catccgggcc gtgctcgctg acgcaacgca tatgctcctt cagccagcaa cgatccatca
    43861 ccacctgcag tccggcccgg cgcgcacgct cagccgccgc ctcgtcgatc acgccctcct
    43921 ggagccagac cgccttggcc ttgataagga tggcttcatc gacgaccgcg cccgcctctt
    43981 cagatcgtct gaaaatattc accatatcga ccggaccggg cacagcagaa agggcaggat
    44041 aagcccgctc ccccaacagg tccgactctc gcggattgac cggaaccacc accttgccct
    44101 gctgctgcat gtaggcagcc acgcggtagg acggtcgtgc cggattcgac gacaatccca
    44161 ccacggcaat cgtccggcat tctcggatca gtcgagcgag ccacgcttgc gaaccctcac
    44221 ctgtcatgga gccccccaga gatcttccag tcgctgcgcc cgcccgcagt tatatttgta
    44281 gaatttgtac cggaccggat tcttcttata aaaatcctgg tgatagtcct cggccggata
    44341 aaacgtcgaa gccagggtga gttccgtaac aatcctccct gggagccgcc cggattgctc
    44401 gatggcgcct tttgactcct ccgccaggcg cttctcctcg tcgccctggt aaaagatcac
    44461 ggcccggtat tggctgccat ggtcgcaaaa ctgtgcgttc ggcgtgaccg ggtccacatt
    44521 gtgccaaaag gcgtcaagca aggcggtgta actcaccttg gccggatcgt agaccacctc
    44581 caccgactcg gcatgacccg ttccaccggc agagacctgc tcatagctgg ggtgttcgac
    44641 ccgcccgccc atatatccgc tggtcaccga tatgacgccc ggcactttct cgaacacttc
    44701 ctccatgcac cagaaacacc ctccggcaaa atacgctttc gccggaccgg caggacccgc
    44761 ggcctgcccg accgaagcca gtgaacctgc cagcaccatc gccagaacca gccacgtata
    44821 catgcctcgg cgtatcacga tattcataga ataagtcctc cctgcaactg acccgcctcc
    44881 tcgctgttca tcttaccaaa gcactcatcc cactgcgatt gcagtgccgg agctgtttct
    44941 catatctacg agcgtcgcaa tcgattggat gtttctgtat gatgggcagg atgagacgac
    45001 tggcttcgcg tgtgggactg gtggcggtgc tgacgctttc cggatgcgcc tcctggttca
    45061 tgaaagggga agcgccggaa gtcctcgtga ccaacgtcac gccgctggac gccagtgcct
    45121 tcgagcagcg acttcaggtg gatcttcgta ttcggaaccc caatgacttc gatctcgccg
    45181 tcaccggcat cgacttccgg ctcgatctca atggtaaccg gttggtccgt gggctcggta
    45241 ataagggact gatcgtcccc aggctgagcg attccgtcac atcagtcgaa acaagtacct
    45301 cgaccttaca ggtcgtccgc cagctactgt ccttctccgg cgaccagccg ctcacctacc
    45361 acgtgacggg cgtgcttcat accaaggaag gccgcctccc cttcgacagc tccggtgtgc
    45421 tgctggaaaa aggcgcgcta tccggatctc ccgctgctcc ctagaaggct gcccccttct
    45481 gtcgtcgttc gtgaagcgtc gtccgtctct cgtcgcaaag cggctggagc gtgcgaacga
    45541 gatacgattc acgttttacg ggcattgaga acgaagcagg cggacttttt caacagcttg
    45601 ctaacccgga ctcttcatgt agcccgtcgc gaggcgttcg agcccgaagc ccgccgggac
    45661 tgctcgcacg tgaggccgac gcaggcgtat ttgcagtccg tcgagaaggc cgaatgagaa
    45721 cagcccctcc agtccaaggg atcgcacgcg ctcacaagct gttcaaaaag gccgtccagc
    45781 aaggccgcag caatgcgagg aggcgaggcg tacccttgcg gtacgttgag cctcggagcg
    45841 gggcgagtac gccgctggcg ggctttttca acagcttgtc agacgccacc ccaggaacgc
    45901 gccccctgca ccaaatccgg ttgctgctgt tcgaactgtt tcatcatgag cccgatggct
    45961 tcttccggac tgtccacgga agcgatcaac cgcccaccga gtttcttgaa aaatttctcc
    46021 tcggccggcg tggccccgac cagaatcacc ggctttcccg ccttgagcgc gagcgccacc
    46081 tccgacacgg tgccggcccc catcagcccg cagaccacca ccacattgct ggacatgacg
    46141 ttgacgttgt tgcgggcatt gcccatttcg gtgatgatgg ccagatcgac atacttggac
    46201 acgcgttcac gagccgaagg cagcaccccg atcgtcaggc tgccgggaac cttcttggcg
    46261 ccctgacagg ctgcatccat cacgccgacg tcacgaccgc cggtcagcac cacccactcg
    46321 cgtcgcgcaa tcaattcgcc cagcacgcga gcattgtcga catctttttt cctggccttg
    46381 gccggcccca tgactcctac aatgtatcgc atgcctatcc ttgggttcgc ccgccgcatt
    46441 gcggctgcca ccagccggtc accgccaccg tcgcgtaata aatggcccag aagtccggat
    46501 cgtcggcaag cctcccatac tgctccatat cggactccgt cactagtcct gtcgccacat
    46561 acttcgcttg aagctgctcg gcagacaatt tcatcatctg cggaatcagg tcgccgcctg
    46621 cggataaatg cccctcgtgt tccaccgtca cctggtccag gtggtggcgc tggaacaaca
    46681 cgggcaactt cgcgcctaag gcatggtcca tcccgaggga cgtgaacatc cgctcgatcg
    46741 cctcgcttac tcgcccgaag gccgcccggg cctcgtccgg tccggccacg gcgcgagccg
    46801 cttgaaaatc cggctcttcg atcacgaccc aaccaccagg tttgacgcac cgcaacatcc
    46861 gctcaaacgc cgtctgatag tccgcgatat gaatcatcac ataccgcgca tgcaccagat
    46921 cgaacgcggc gggcggaagc tccatgtcac agatatcccc ttgcaggatt tcgatggttg
    46981 agctgccgct cccgcgcagg aatctcgggt tcgtgtcgac cgcgaccacc ttgccggatg
    47041 gcccgacccg ctgctccagc caacgcacga tggagccggc ccctgctccc acttccaggc
    47101 agtgccaccc ggtcgtcagt ccggtggcta acatccgtcg ttgcgtgccc ggatcgaaga
    47161 tccgctccag catctgcaac cgctctaatt cacgggcctc ttcagtggca tgaaacacgt
    47221 aatccgtctt cagcatctcg tatcctttgt ctggcacaga gatgggctcc ggggcccgaa
    47281 ccggcgcggg actctaaaat cgagaccccg tcaatgtcac gtcggcctgc gcccatacgg
    47341 cacgaaaccg ggcttcttcc tgggttgcat ctttgttctg agcccgcagg ctctgcatca
    47401 gtccgtgcaa cgcccatcca ttctcggggt tgaggcgcaa gtcttcccga tacacggctt
    47461 cagcctcggc cggacgttcc gccagcagca gaaccgctcc caggttgtgg cgaaccggct
    47521 gaacccagaa cggcggctcg ctgtagggca acgacgcctc caactgcagc gcctcgcgca
    47581 aggcctgaat tccagcctcg tattgctcgc gccgcgcggc catttccccg accaggaccc
    47641 gctcggccac cttcaacatc gtgcgactga tcttgccttc cgccgtcttg gcgcgagcga
    47701 atggcttcat cgccttccgg agattcgaca attccccctc ggcgctcccg aatcgtgtcg
    47761 tcgcggcaaa ggccaacccg cgcgaaaaat gccagagacc cgtagtaagc cgcagactct
    47821 tggacggcgg cggatgcctg atcaactccc cccactgacc gaagcggacc atggtcagca
    47881 gcacggtcgg cgcataccat tccagcgacg gctccttggc aatctcgtcg cccggaacct
    47941 tcgccaggag atcccgcgcg acttgcaggg cttccgcgct ccggccttcc atcatcaagg
    48001 aggcattcag gaaatggagg ttgtgcgcat agtatcccga tgcgtagttg ccctcaaggg
    48061 ggtgacgaga caaatactca tgatccacca tcgcggcggt cgcattgcgc tccgacgcct
    48121 cccgataacg ccccagccgc atgtagatat gcgccggcat atgcaccaga tgtccggcac
    48181 ccggcatcag cgagggcaac cgcttcgcac agtcgagcgc gcgctgaggg tcgggcgaag
    48241 cttcgaccgc atgaatgtaa tagtgacagg cacccggatg atccgggtgc tgccgcagcg
    48301 ccgcttccaa gatggccagg atctcctccg tccccggttg aggccgtcca tccgcgcgcc
    48361 agtagtccca gggccggagc accatcatgg cttcagccgc tatggtcgcg gcatcggcat
    48421 cgtcgggagc ctctcgcgcc acttgacgca tcgctttggc ataggcttcg tccttcgcct
    48481 tgcgggtgac cgatttcttg gccgagtagc gagcggcaag ggcttccacg taggcgcgct
    48541 ccctggcggt ggcttgcgac agacgcgctg tggccttctg catcgcctcc acggcacgtg
    48601 gctccacctt ggggtccatg gccgcgttga tgttcggccc caggctcaac gccacacccc
    48661 aatagggcat gggcgcatcg ggatcgagac gggaggcctc ggtgaaggcc gcaatcgcct
    48721 cctcgtgatt gaaggcatag accagccgga gtccttgatc gaaaaatttt tgagcgaggt
    48781 ctgagttggt cgtgatctga tgatgtagag agccgagatt ctcgaacagc gtgacctccg
    48841 cccgcgcgac tccacccgaa ccaggcaaag ccaaccccgc gaccatcaag gcgtgaacca
    48901 gcgccctcgc ataccgcccc tttaccatcg cccttctcct gctcggtttt tcgtatgtcg
    48961 gacggaccac gccgtcacct gcgacgactc caccgcccca cagaacggca caaattagca
    49021 gtcccgtcga gaacttgcta gtcggagcac gtcggcaagg gaagatcggt tggaatagct
    49081 cgaatgcggg acgctcaaaa cggtcgtcca gcgaggccgc atcgagtgaa gggccgaggc
    49141 gtaccctctg gggtacgttg aggtcctgaa cgatgcgaga acaaagctgg cggtcgtttt
    49201 cagcgtcctg ctcgatcggc aggaatcagg cgttgaggta gggagtctgg gatgcttcgg
    49261 cagaagcctc ccgtcggtct ctcgaagatt tcttgtcacg gtcacggtat ggaatcgcgc
    49321 gtgacggcac gagggagagc tccagctcgg caggctgcgc cagctccatg tccttgccca
    49381 tgtacatcaa gaccttctgc tgggaggtca ccagagcccg caaatcttgc agctgcgccg
    49441 tcaactcttc gacctggacc tgttgagtcg acacctgttc ccgcaattga tcgacttccg
    49501 cctgggtgga ccgggtatgt tcgatcaccc actccttgag ctcacgagcc tgcgcggtca
    49561 acgcgcgttg cacaaagccc gcacggtcgc gcaaccggaa aatgagatca ccgactgcgc
    49621 catcccaccg acgggcggga accagcccgt gttcctcctg gacctcatgc tcgatatgac
    49681 gcgcgacagg agctcccagc catttcgaga cccgttgtcc caacggctgt tctgtccggt
    49741 cctcagagtt gccgtactgg caccaatcct cgaaccgctc ggtcatatcc tccagcttga
    49801 cgaacacctt tccgatcgcc gcgcgcaacg gcgaaggtgc aaacggattc acgccgccct
    49861 tctcggactt gtccggagcg aaggtgagga gatgaatcgg tttttcggcc aaacgctgct
    49921 gcttacgctt catagggaca ccggcatctt atcggtcggt actcgatcat tcattactct
    49981 ttccaatagt ccgagtcaat ggccggaaca ggttttttgc cgtccggagc ccccggattc
    50041 ccgtgacctc gaccaggctt agaatcgagt gacgcttgaa aaattaaagc cgtggatccg
    50101 catggcaggc accaggaact tcccggtttc cgacgtgacc gcttcgaccg gggcggtgta
    50161 ggtttccacc tggttgaagg cgcgcagggg gctctcgtga aaccggaagt tccggacggc
    50221 cgacgtgatc tccccgtttt caatgaggaa ggtgccatcc ctcgtcatgc cggtgagtgt
    50281 caggtcggac ggattgacgg ggcggatgta ccagaaattc gtcaccagga tgcctcgctg
    50341 ggtcgtgcgg atgaggtcgt ccacccttgt gccgacagga cgttcaccgg ataagtaggg
    50401 agactccagc gtcgtcagtg gatcgatccc gtgttcctgc gcggtaaacc ggtcgtgcaa
    50461 caactggttg agcacccccg cctcaatcca actggattcg atgaccggga ggccctcaat
    50521 cgtaaatccg tggccgagaa ggtcggcatg ggccggctga ttgaacaggg acagtcgacg
    50581 gtcgaggatc cgggtgttca gttttctgct gaacggactc gtccccttgt agaaggattt
    50641 cgcatccaac atccagatca tccagctcag cagcccggcg acagccgccg gctccaggat
    50701 gaccgtgtat cggccgggcg gcaattcccg cggttcgtcc gcccctcgtt tggccttgat
    50761 gatggccgcc agcgtgcgct cctgcacatg caaacgatcg atggatcgat gggccgccgc
    50821 cgaccagccg gtggcctcgc cggcctgcac cgtcagactg aaccgcgcct cggtgcgttc
    50881 ctcgtaggcc cgcaatcccg tgtcagccgc caaccccacg atggccgtgc tggacgacac
    50941 cgttccggcc gcgttcagat tctccatctt gcattggccg atggcctcac gcgcatagtc
    51001 gagccgccgt gccggtccgg ctgcgctcgt ttcgggacgc gaggtcggca gcgatagata
    51061 ggtctgtggc ccgacgggag gaagatattc gggatcttcg ggagagatgc gggcaatgca
    51121 ctcggcctgc ttgagggttt cccgcactgc gccggcggta aaatccgtcg tgctggccgt
    51181 gccatgttgt cgcccgaagg ccaccgtaat cgacagggcg ccgcgccgct gattcacatt
    51241 ctgaatgacc tgattgttcg cgaagcgagt cgtgccgcca tggacatcgc gcaacgacag
    51301 ataggtgtgg tcacccgtcg agtgactcat caccagctca gccaggaact caaattcctg
    51361 ccggcccgtc aacttcggcc atgatggagt gatcgacgac gtcattcgtt ggccttcgct
    51421 tctccggcaa tgacatccac ccgcctgaac cgggcatggg aggcggcatg ggtcatccag
    51481 cccgattggc ccggttggcc tttgccgcag gtaatgaatc cataccggcg acgatggctg
    51541 cgatcggcga cgccgtcgca tcggccccaa aactcagggg taatgccatg atagatcaca
    51601 tcccgcacca tgtgcgtgcg gcgaccgttc tctatcagcc agaacgcatc gcccccgaac
    51661 tgaaaattgt atcgtcgctg atcgatacta taggagccgt gaccttcgat gtagatgccc
    51721 cgcttcacat cggccaagag ttcatcgagc gtcgccgtgc ccggctccag cccgatgttc
    51781 gcaatacgca ccatcggcac gctcccccaa ctatcggccc ggttcgatcc gcgtgaacgc
    51841 tcctcgttga tacgcggcgc cacctcgcgg ttcgtgcagt acccgacgaa gagtccctcc
    51901 cgcacgatgt cccacttctg gcaactcact ccatcgtcgt cgtaccccgt cgccgccaac
    51961 gtgtgcggtt cagtgttgtc ggccaccagg ttcacgtgag acgaaccata ccggtaagtg
    52021 ccgcgtttat ccggtgtcag aaaactcgta ccggcgtaat tcgcctcgta gccgagcgcg
    52081 cgatccaatt cactgggatg tccacaggac tcatgcatcg tcaacgacag atgctccgga
    52141 tcgagcacca gatcgtatcg cccggcctcg atcaccggcg cccgcacctt ctccacagcc
    52201 tgctcggcga tgcgcgcgcc ttcgctcgca aaatcggtgt cgcggatcaa ctcatacccc
    52261 atgcgtaaat gcgggctgtt gaacgaacgc gtggcaaaac gtccatcctg aaccgctgtg
    52321 gcactgcact caccctgcac cgccagcaag ctgaactcaa gatgggagcc ttcggtagag
    52381 acaaagagtt tacggtcacg ttgggcccag aaactggcgt ggcttctgac gacgccctgg
    52441 tgtcgctgaa tgacatccat cgtgctcagc agcaacgacg tttgttcttc cagaggaacc
    52501 gcgaagggat ccagtcgaca gggagtgacg atacgatcgc gatgcacggg ctcaggcgcc
    52561 agatgtacct tcgtgatggc gagcgaggcc gatcctttgg caatttctac ggcgaggtcc
    52621 gccacacgtg gaatttcttc gagggaaatg acggagctgg ccgcaaatcc ccaggcgcct
    52681 cgatgcagca cccgaatgcc gaacccacga tcggtgctct cgcgtatgtg ggcaatgcgc
    52741 cggtcttcac cgctgatcgt ccgcgtcgtc gtatcgagca gacggatatc cgcatactcg
    52801 accccggccg cgcgcacacg agacagcgcc aattcggcca actcgtccca cagcggtcca
    52861 ctcatagcag catctctcgt tgcggaagga agcccggaaa gtcttgcgta gtataacaag
    52921 atcgcggggg ccgattcctg tcttttcatc gacaggccgt tcacctatga tgctccccct
    52981 atgttctccc ccgaacagac accgcgacgg cgaatcatcg agctgctgac cggcacattg
    53041 ttatcttccc atcaattggc ccagctcctc ggcatgccgg aacgccaggt ggaggaccat
    53101 ttaacgcaca tcgtgaaaac cctgtcgcgc gatcccgcgc ggacattcct gattgaaccg
    53161 tcggaatgcc aggattgcac gtacgtcttc cgcgagcgaa ccagactcac ccgtcccagc
    53221 cgctgcccgc gctgccgcag cgaggccatt acctccccgc gattcggcat ccgcgaggaa
    53281 gaccgcgcgt aatagacatg acggaacagg actgccttct tgcaaccgcc gatgtcttga
    53341 gcgagactgc gcagatgcct catctcgtga cgacattgcg cgacatgtca gccgaaagga
    53401 ccgccgcatg aacagagtcg ctgcctggat catcccgagt ctcctcctgg tcctgcccgc
    53461 ctgtggtccc gaccaggctg cgcagctgct ggagacggcc cagttcgaag aacgccagac
    53521 caatcgagcg cacgccaaag aactctacga agaaatcgtc cgtcagtacc cagacagccc
    53581 cgccgcgaaa acggcgcgtg cacgactgac tgaactcacc gacaaaccgt agcggcagcc
    53641 atttatcccc ttgtgtagcc gcaggatgcg caaaaagttc gtccagcaag gccgcagcga
    53701 tcaaagaggc gaatcgtact cttgtggtac ggcgagcctc gacgagaagc gagaacgccg
    53761 ctggcgtaat ttctcggcat cctgttaacc cggattattc atgcatgccg tcgcgaggcg
    53821 ttcgagacgg aagcccgccg aggcggctcg cacgtcaggc cgacacaggc gtactggcag
    53881 tccgtcgagg aggccgaacg agaacagcct cgccgggcga agggatcgga cgccggagta
    53941 ggtattcatg aatagtacgg gttaagaccg tcgcggaaac cagggaaaga acgcgttggt
    54001 cgttcgctga tactccgcat attcctcacc acggctcgtc atgctctgcg cttccgtcca
    54061 gggaatgccg gtgaccttca gcagcgctac gcccatgacc atgggaccga tcagcgtcag
    54121 gccccagcct cccgtaggaa tcgccagcgc catccacaca taactccacc agtgcagcca
    54181 ttcgaaaaaa tagttgggat gccgtgaata gcgccacaga ccgatccggc acacacgacc
    54241 ggtattccac ggcttgctgc gaaacgcagc cagctgccaa tcggccacgg cctcacccat
    54301 gaccgccgca gcccaccaga gaaatccaat caggtcccaa aaatgaaacg gcggatgggg
    54361 gttctgcatg accacaagcg gcgggagaga aaaaaacgcg atggcggcag cctggagctg
    54421 gaaataccag aacatgcgcg aggactcgtg cgcgccccat tgatggcgca aagcccgata
    54481 ccgcccatcc tcctgcttgc gccacacccg gtcgatgagc acgtgagtgc ccaggcggac
    54541 ggcatagaga aagatcatca acgccaccag caagcgcctg gccggttctc ccgcggcgga
    54601 gcctgcgtac caccacacca ccagtgccaa cccgtaacac catcccacat cggcaatgga
    54661 cgcgttacgt ctgcggcgtt cgactaacca gagcaggacc atcaaggctg cggacgcagc
    54721 ccaggcggtg atgaggaggg acaggggatc tctcgctgtc atgcgatcag ctaccggaca
    54781 tgaaccgcaa gcgaaccgga tgcaccgcac agaatcggac aattgtacta tgacggcttc
    54841 catcaagaca gggccgccgg agtcgaatgg cattcggatt cagaggcagg tatgattcac
    54901 catcggcact gcggcaagca ctgtcacatc acaccggagg gaccgttatg aacatgggtt
    54961 tcgtcaaagt gctgatcgtc gcgatagcct tcgcggccgc actcccaatc caatccgagg
    55021 ccggtgagaa tgcttccccg aaagcggctc accccgcaaa gggtaagacg ctcttcatca
    55081 aacactgcgc cggatgtcat ggagctcaag ggcaaggaga tggatacaaa ctactggggc
    55141 ccgatcccgc gaatctcacg gcaccggcca ccagaaaaaa atccgaccgt gccctgctgg
    55201 ccaccatcca tgagggcaaa ccgaacatgc cgtcctggaa aggccttctc tccgaacgcg
    55261 acattcgaaa cgtgctggcg tacattcgca ccttgcctca ctgacctacc gatcgcctga
    55321 ggctgagccg tcccaggggt cgtcgccgac cgaggagtca ctattgagcc agtgacgaaa
    55381 acctggcagg cggaacgaca gcagggcctg catcttgtcg cttcgcatcg agaggtccgg
    55441 aggacgaggc gggccgtgat agtcgacaac tgacccgggt tgaatccagg gccggagttc
    55501 cggatagcgc ctcgccagca actcaccgat ttcccatcga ggcaaccgtt cgctcccacc
    55561 caggtgatag aggcccgccc gcggctgcgc ggcaaactcc cacagggcac gtaccaatgg
    55621 tccggccgga agggggcagc gaaactcgtc cgtgaagagc gtcagcttcg tccccttggc
    55681 cgccgtccgt aacatgtctt cgacgaaact ccgatcacgg gtcggcgacg tgcctgctgt
    55741 cagcgcgata cgaacgatcg aatgggcagg attctcgagc accacctgct ccgcttctgc
    55801 cttcgtctgt ccatacacat tcaggggatg cacggcatcc gtttcgacat accagccctt
    55861 cgcgccgtca aacacctgat cggtagacag aaagataaag gggatgtcgc gtgccagtcc
    55921 ggcaagccgt tccgtcgcct ccacattgat acgtcgggcc tgcgccgggt cctgttcgca
    55981 ggcgccggtt cggctcagag ccgcgcagtg gatcaccaga gcgggagttg atcggcgcca
    56041 gagttcctgc acctgcattg ggttagtcaa atctgcctcg gccctggtga ctccgcgcac
    56101 attccactgc ggggcccacc gagcggcagt gctgacgagg tacccgccga tcagcccgcc
    56161 ggccccggta atcaaagcgg tcggtttcat gcgtcactcc ggccgaggcg acggatctcc
    56221 ggcacttcga tcacccgact accggacggt ggctgttcaa tcgcccacag catcgtcatg
    56281 agcatctcgt gaattgtgac caaacccagc ctcagagcgg cggctctcgt tcccgggaac
    56341 tgctccatca gccgatagac cggctgcagc gccaacggcc accaatgacc cggaccgaga
    56401 atataccagg gcctgaggat cgtcgcgacc aaccccgctc ccgcaatggc cgcttcgcat
    56461 tcgctgcgca cggcaatata gtcccgcatg acgggagccg gatgcgccac gctgacatag
    56521 accatatgtc gcacgccggc ttcgcgggct gcacggatcg ctgccacggc cgaagggccg
    56581 tcgatggcgc ggaactgcga gcctttccag ggtgcaggtt tcggcacacc caccaggtgc
    56641 acgagggtat cggcgccccg cacctgctca acgaacgtcc cgctctccag cggattgccg
    56701 acgacgatct ggcatccggt gggaaccttg cagactgatt cctgccgcgc cagggcggtc
    56761 acttgatgac cccgttcaag caacaacgga atgagccgcg cgcccagata gccggttgcg
    56821 ccagtcacaa acacgcaccg ggttttgacg ggcgtcgtca tggatgcgtg agaagtgcgg
    56881 gccgagcact cataaacccg gcccaaccgt ctgcttgatg catctcctcg cggcccttcg
    56941 ccacgagccg agccgctgcg gtcgcgagcg ccgcctgatc ttccgccgac agcccgagat
    57001 tggtcttgat atgggtcacg cggtcgccca atggatcgct gtccggcaac tggcgtaacg
    57061 caatgtgcca gaagcggcag gtcccgccgt gtccgttcat gcccacctga aaggtcccga
    57121 acatcgacgc atcctgctgt gacacaggaa tcccggccag ctccagcacg ttgcgccgat
    57181 ggcctctgag cagagccgca gcggcggaga gaggtttgcg ccgctcattg agctgacggc
    57241 ccgtcgcatc gatagaaacg accagacacc cgcgggggaa ccggtcggcc atcgattgct
    57301 ccgatagcgc ctcgttcacc acctgcatca gcgtccggat cccgagattg tctgcagccc
    57361 cgccgtcata ggccagcagc gggggctgcc cggatggcgc gccctctgcc tgcccataag
    57421 gaatggccag cggttccaac acaccgggat acgcggcgga catatacgtc gcacgcgcaa
    57481 tgctgaaggg agcgaggggc tgacccagcg cagcgaaccg ttcgtcggaa atgacgagcg
    57541 ggtcgccggt gagggcatcg gtggcattca gcagaagaat cggttgcgac ggattgagat
    57601 cggcgaaggt tgcgccccga aaaagccggt cgtccaggac gcgaatgacc tgttcggctg
    57661 gaacccgatc ggtcaaggca taccggatcg cgttctgggg aacggtatac cagggaccca
    57721 gcatcgcgcc ttgaaaatcc cgtccgacca actccgcaaa cccgttctgg aaattgaaat
    57781 cacgataccc ctccagcgca taggacgccg caggcagtgc cccgccggat actgcagaca
    57841 gcacatcgac ctgttgaagc acacccagcc ggtcgagttc tttcatcacc gccgcgccga
    57901 atacggctgc gcggctccct cccccggaaa aggccagccc gacgaaccgg ccatccgcca
    57961 gttccttctc tcccaagtgc agcgtggctc tcgtgtgaag ggggcgaaat tcgggaggcg
    58021 atgccggggg aggatcaatg gccgtgcagg aggcggaggc cagggcaagc accgccaacc
    58081 agcggcttag agtacgttcc cgtaacatgc tcatggatga atctacggcc gaatgcggaa
    58141 aatggatgtg cctctagtaa ggaatcctga ctttggggcg gacgctaaca ggatgctgac
    58201 accgtacgag catacagcag ccatgtgact ctcaaagact catagactcg cgcagtggcc
    58261 gtcgcaggct gttcaaaaag gctgtccggc aaggccgcag ccagcggaga ggcgatgcgt
    58321 accctctgcg gtacgtggag cctcttcgcg ccgcgagaac gccgccggca gccttttttc
    58381 aacagcctgc taattcctgg tcagtttgcg atagcgtatg cgatgcggct ggtcggcttc
    58441 tttaccgagc cgtttcttgc gatcggcttc gtattcgctg tagttgcctt cgtaccacac
    58501 cactttgctg tcgccttcga aggccaaaat gtgggtggcg acgcggtcca agaaccagcg
    58561 gtcgtgactg ctgatgacgg cgcagccggc gaagccttcc aatccctctt ccaacgcgcg
    58621 caacgtgttc acatccaggt cgttggtcgg ttcgtcgagg atgatgaggt tggcaccttc
    58681 cttcaacatg cgcgcgagat gcacgcggtt ccgctcgccg cctgacagat ccttgacctt
    58741 cttctgctga tcggtgccgg cgaaattgaa gcgggcacag tagccgcggg cattcacttc
    58801 ggccttgccc agcatgatcg tgtcttgtcc ctcggaaatg atttcgtaca ccgtcttgtt
    58861 gggatcgagc gaccgatcct gatccacgta accgagcttg accgtttcgc cgatgcgaat
    58921 ggtgcccgtg tccggcttct ccttgccgat aatcatgcgg aacatggtgg tcttgcccgc
    58981 accgttggga ccgacgacgc cgacgatgcc gcccttcggc aggctgaact cgacattttc
    59041 atagaggacg ttgtcgccga aggccttgct gatgcccttg gcctcaacca ccagatcccc
    59101 gagccgaggt cccggcggaa tatagatttc cagatcggcg gccagctgat cctgcttctg
    59161 attgaccaat tcctcatacc ggttgagacg cgccttgccc ttcgactgcc gggccttcgg
    59221 cgacatgcga atccattcca actcgtgttc gagcgttttc ttgcgcttgg attcggcctt
    59281 ctcttccttc tctaaccggt cctgcttctg ttcgagccac gccgtgtagt tgccttggaa
    59341 gggaatgccg gagccgcggt cgagttccag aatccacccc gccacgttgt cgaggaagta
    59401 gcggtcgtgc gtcaccgcga tgaccgtgcc cttgtactgt tgcaggtgct gctccagcca
    59461 ttgcacggat tccgcatcga ggtgattggt cggctcgtcg agcagcagaa tgtccggctc
    59521 ctggatcatg aggcggcaga gggccacgcg gcgtttttcg ccgccggaca acacactgat
    59581 cttctgatcg gcaggcgggc agcgcagcgc atccatggcg atttcgagtt cgctttccag
    59641 ctcccagccg ttcgctgcct cgatcttttc ctgcagctgc gcctgcttgt cgatgagctt
    59701 ttccatctca tcggggccgg cctcgcccat actgttgctg accgcttcgt actcgtgcag
    59761 cagcgccacc agctccttct ttccttcctc gacgacctct ttgaccgtct tgttgaggtc
    59821 gagttgcggt tcctgttcga gcagtccgac gctgtagccc ttcgagcgag tgatctcacc
    59881 gacgtagttg ggatcggtcc cggcaatgat tttgagcaac gagctttttc cggatccgtt
    59941 caaccccagc acgccgatct tggccccgta gtaaaagccc aggtagatat cgcgcaggac
    60001 ctgcttcttc ggcggataga ccttgccgac accgaccaga gaaaaaatca cctgcttatc
    60061 gttcgtcgcc atgcgttccc ctatctgatg gtgtggtact gtgttgagaa gaatacgttg
    60121 cgcggccgct tacaatgagt gcacgagttc ggccccggcc ttcgcccggg cggccatgtc
    60181 ggccaggccg accctgacct ctgcggctgt gcggaccgga ttggtgaacg cgacccgtcc
    60241 cccctgcatc cgctcggcgg tcttgatgcg cagatgaaaa cccagacgat ccagcgcggt
    60301 catggtcacc tgctgcgcct ccagcttgcc gtacacccgg gccaggagca acagcgtggc
    60361 tgtctgctgt tcattcattt cacggatcag cccagatgcc tgatcggcta gtggatccac
    60421 cgcagcggcc gtatagtcgg acggaacgac ccagcccatc gagccaaacc cgccgacgaa
    60481 atagatatct gcgatcgcca tgcgaaagaa gccgaagtcg ttgaagtcca cccaataggc
    60541 ggcgttagca tgccgggcca ggtagcgttc gcgaaccggc gcactctcct ccttcggcac
    60601 acgcgtcacc gatcccatca acgtcaccct ggcggcaccc agaggatcgg ttctactttc
    60661 cggcggcgtc accagcagac tggcgcgtgg atcgcccagg aggttctgcg tgtgcatcgc
    60721 catggtgctg atgagaaaca acggttgccc ctggtcgtcg aggccatagg gcatgacgga
    60781 cccgaagggc cagcctggtt gtttacgcga gatggtcgaa aggccgcccg tctgctgtag
    60841 atagaccaga gtctttgccc gctcggcatg ggacggttca gggacctcag gaccgtcgga
    60901 atccggcccg ctattgtgtt gctgcgttga tgacaaggat gcctcttttc tgtgcaataa
    60961 gctgcacgac accttacctg gatgcttccg gaatttccac tgagcgcggg gggaacggga
    61021 ccggcacaac gtccgtcaga tgccgagatc ttcggtgagt cgcacttcgg attcatcgag
    61081 gatgtcttcc aggcaggtga agcaggtgaa cgggaactga ctcaccggaa tcgcgcacac
    61141 ttcgccgacc ggcgattcgt ccagcgccgg gccaccgcac tctttatgga tcaagaccag
    61201 cataggcgcc cctctatcgc gatcggctca ccaactgcct gaacgtctcc aaatggtcgg
    61261 ggctgctcag acggtgatca ccggttttga ataagacgtc gatgggaaat tcaggatcct
    61321 gtgacagcag gtgctgcacg aaggtccagc tgccgtcggg cttgatcact gtatcctgca
    61381 gcccgtgcag aatggtcgtc ttgacgcgac gcggaatggt gaggcgacgg ctccagaggg
    61441 cctggctgtc ttcgacccag tgccagggga tcggcgcctc tcgatgcatg ggctcgtcgt
    61501 cccatggcat ggagccggtg gcctgccact gctcacgccg ctcattggaa atctgagccg
    61561 cgcgcagatc catcatattg aacgccgggg cgatgagaat caattcttcc accgccggtt
    61621 gctcctgcgc aaccagccag gccagccatc cgccgaggga gttaccgacg atcgtcaccg
    61681 gaggcccacc cgccaacaga ttcgtgacgg cttcggcgtc ttccagccat tgcagcagac
    61741 gatactcacc ccacgcaccg tccgactcgc cccagccgcg aaagtcgaag cagcagaagc
    61801 cccagccccg ctcctgacac cattgcgcca gcgccttgct cttgttgccc caccgcttgg
    61861 agagaaaccc ggtgatgaag agaagctggc ggtccttgcc gacgacacga tcgccacgga
    61921 gagttgtgcc gtctgcaccg gtgagtgtga atggatccat cccgcgccta tctcacggcc
    61981 ttcaagagaa aactgcccag cttgcgcaac accggctccg gaatttcatg gccgccccga
    62041 aacggctgcc aatcgactga caccccggcc tgcagaaatt cgtcccgcaa ccgttctgcc
    62101 atcgcgtagg gcaggatcgg gtcttgcgtg ccatggctct gaaacaacgg caggcctttg
    62161 cgtttcgtca gcaacggcgc ccactcctgt tttgccacca gggtccctga gagctggatg
    62221 agtccggcat agggctgagt gctgtggagc agtgcgtcac aggacagcat cgccccctgt
    62281 gagaagccgc ctaacaccgt gcgcttggga tcggcgccga gtttcttttg tatctcatcc
    62341 agcagcactt tcatatgctc gcgcgcttcg gtaagcccgc gcgggatttc gccggacatg
    62401 tcccggactt tgcccgccgc tcgatcggct tggatacggg ccatgtcgat catccaccag
    62461 gcgcgcgaat cgccgaagcc catcggaatt gaaatcggcg cttcaggaaa gagaaagcgt
    62521 gtcccggtgg gcgcatcgag atattcgctc agcggcacga gatcgtcgcc cggcgcaccg
    62581 aaaccatgga gcagcaccac cagcgggccg ttgccccctc ccttgtcgtc gatgcccccg
    62641 gtcagacgaa ccgtgagacc accgagtgtg tccatacgca tcgacgtgct tctcctattc
    62701 cgagagttgc gtagatattc tcgcaccgct atcggccgaa tcaacagcga tgcccctgca
    62761 ccggcgcccg atcaccagcg cccaagcaga cgttcgcgac atgaaccgag gattactggc
    62821 caagatacca atattgaagg aaggaaatca ccgcgaggat gctggcacta aataccggat
    62881 tgagaaccaa tggcgtgtag gcccctttct gaacgtgctc gatgtcctct agtgccaggc
    62941 gcagctgatc tgcacaaacc ttttgttgcg tccccattcc tgttacagca agcaggtgct
    63001 cacgaaaata tccaatggca aggtcacgag cttgtctggc tctcgatctt agaatgatcg
    63061 tactaccggc cgccagacca gcatagatga gccaaatcat aatcagcgcg agaggaaaat
    63121 cccaattgtc gaaaagccga ttccgagaca atatcataaa gaacaaggcg atgaaggggc
    63181 aaaatacgag tttatgcacc gcgtgagttt gatccgcaat gcacctcacc ataatcaaac
    63241 ctggaagata ccgacgatct aactcgaacc gggaatgaaa tttagcaagc gtcttatccg
    63301 gccaccgtat ggatgtcgtc ttgagtcgtg ttatgaagct ctggctcagc aacacggcat
    63361 cgaacacaaa catgtttaag aagaccatcg ataaaactgc cgcccacgtc acaccagcca
    63421 ctattttgca gttagtaatt cctcggcagg ggctggatgg cggaccaggc gatgcaatga
    63481 cagccaaaat gatgaaaatg agaaatagat aaattacgag taagctcaga gatcggacga
    63541 ctcggtttct atagagagcg gcccgtgagt aatcgtgcca tattcgagcg atgctcgcac
    63601 taccatcggt tggttgcggt accgacttaa tccaaattat ggacctaaga agcacccata
    63661 cctgttcacg taggctgcct ttgggtctat aggaagggag cgcaaattct tctttcaatt
    63721 cactctgact ggatcgaagc gcatagtagg ctctgaccaa acatccaaga caccatgcaa
    63781 tcacgagaga gcggatgatg atgcttggcc aggtgctcac cccgtcggaa atgctgaacg
    63841 gctcaccctc ccatctatcc tccagaatca cccgtatcag ccgatccact cctcccacga
    63901 ccgtgaaagc tccgacactg gctattattg tcagcgcaac gaccctccaa tgccatttga
    63961 ctctttcagc gacatcgccc ttcgcccaat tccacatttt cccaggcagt tgagtgaaca
    64021 cgacaagact cactagcacc gccaccagga gccagaacac agtgtgacgg aatcgaaggc
    64081 gataatccgc gttcaaaaat ttccaccagg gtctcatccc cagaggaaac agttcacgcg
    64141 ggggctgaat catttctatt tctcccaccc gcgacgggac ggctcctcgg tcagcagaga
    64201 ggtcgactgg accatggcgc ccgacctcga atagtcgtgg acaaagcagc gcggagaacg
    64261 gtttcggact caaatcgatt tcgtatcggt caaatagtaa ctccatactc tcaaaaccag
    64321 tttccggaca caccattgtc gatggtgtcg acgaccgcac agacctgatg tggcccaagg
    64381 ctcgaagcat cgtaaaaaaa cctgaggtct gataactgtt ccggaaaggg gggatatcat
    64441 gctggattcc actatggaga ttcagaccaa agggtgacgc gacaacgagg ttgcgcgccc
    64501 actttgattc acgctcctga aaaaaccgcg catccaaatc agttgtaaaa aaaatcgcgt
    64561 tgggaaattc ttctcgaaca gccctcagaa tgagcaaggt gtcatacacg tcgctgccga
    64621 gcaccccgat cgccttcggc ggcttcccaa tggacccgct ggcctcgtca gcatcctgct
    64681 tcattcttgc aactaacctg cgcagataat ccagctggct ctccccatcc gggcgttcga
    64741 ggccttcaaa cgtcttatct tgatgcttct tgtcattcga gttcgatcgg ccgctttttt
    64801 tatcgttctc tgcctccccg cttttcttac tggctcctgg tgtcaggcca tcgaggccgc
    64861 ggagatagga atagcgggag atcctcatct caagcgcatc ccaacgttct gggtg
//
LOCUS       DAORRQ010000020        61911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_25_length_61911_cov_736.223438, whole genome shotgun sequence.
ACCESSION   DAORRQ010000020 DAORRQ010000000
VERSION     DAORRQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 61911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61911
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25_length_61911_cov_736.223438"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(607..822)
                     /locus_tag="PK224_08425"
     CDS             complement(607..822)
                     /locus_tag="PK224_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15802.1"
                     /translation="MPVRAGGDCFRIGILGPHRILTGSLQLALRQRHKFVDILRATAA
                     ASVIAQAEFSNDLSGGQEALTTGRPHI"
     gene            complement(822..1433)
                     /locus_tag="PK224_08430"
     CDS             complement(822..1433)
                     /locus_tag="PK224_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRB15803.1"
                     /translation="MIQLGRVLLLCVSSLLAWPIIGRAADSPLAINVLVAYHSLSGHT
                     ERMAEAVAEGARSGPQTHVVLKRAGKVTAEELFAADAIVLGSPVYWSNMAGEVKTFID
                     DWQFKFGVFPEFKLKNKVGAAFATGGQISSGKELTMLSILAAMLGNQMIVVSGGGAFG
                     ASATTEGDSPGIDAQELTGARELGRRVADVAGAVKRGFESQGK"
     gene            complement(1576..1761)
                     /locus_tag="PK224_08435"
     CDS             complement(1576..1761)
                     /locus_tag="PK224_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15804.1"
                     /translation="MKETRRVVYMRGVFVCPKCRQSYVQEKWIEEFRIRCHKCDYRGT
                     LTQVSVEEHMDEETVRR"
     gene            1900..2412
                     /locus_tag="PK224_08440"
     CDS             1900..2412
                     /locus_tag="PK224_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRB15805.1"
                     /translation="MTLDTTDRLDNTHCPALLRRAMWCLTGITMLSMTCFSLAADPQA
                     DTLITVKTPTGTLIQAELADTALKRAQGLMFREHLADDRGMLFIFGDAQPWTFWMKNT
                     KIPLDIIWMDAKKTIVHIERNVPICTRQDDGCPQYHSDEGALYVLELGGGRAAALQLQ
                     RGMKLSFKQP"
     gene            complement(2429..3943)
                     /locus_tag="PK224_08445"
     CDS             complement(2429..3943)
                     /locus_tag="PK224_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRB15806.1"
                     /translation="MSLGLLVGTVLAMLAGMAAVLMGLTYLLARRGRSAPPLPFYAGG
                     VALVLVTAALVGRSELVSHSPHFLRSGLDVAAWLGASFFLFTILDALIIGEWLIERGQ
                     RYIPDIVRQLLIGAEVVAAGVLILWLVMGINLVALVALPTVAAAMVGVALKDTLTRFF
                     SGIELGKIVKVGEWITVVDREGMVTHIGMEHVTLLTRAQDLVSLPNDLVIQSGVTNFS
                     RPTTTHFCNVYVDAAYRTPPEQVCATLLEAAVAVPGVLQDPKATALVTAFNESAIQYR
                     IKFPIGDFAQRDMIESRMRTYIWHAFARNGIEIPFPQRVVHQTVEPEVVTNGQAVGRM
                     VAQLAAVDFLGTLDAKQLEALAKEARWESYLPGERVVRQGEPGEVLYVIASGKADVRL
                     EQGGLSSTVTTLEAGKFFGEMSLLTGEPRSATVSAATELLVIAVGKQSLLQVVQEDRR
                     LIERIGEVVARRQAATAAAKAQLSREAAALSAVTHTRSLVERIQNFFWGARKAQ"
     gene            complement(3992..6175)
                     /locus_tag="PK224_08450"
     CDS             complement(3992..6175)
                     /locus_tag="PK224_08450"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRB15807.1"
                     /translation="MPHETVTELSQLIDRVKSYNVEADLDLVRRAYDFSAKAHEGQMR
                     RSGEPYFRHPLAVAGLLTHLKTDVTAIVAGLLHDTLEDTLATPLELEQRFGKDVVHLV
                     DGVTKIGKITFRNYEEKQAENFRKMVLSMADDIRVVLIKLADRLHNMRTLEYLSEGKR
                     QQIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYESLRVRVAKRDEDRQQYI
                     IEVIDLVKKAMAEAGLQGEVSGRPKHLYGIYQKMEKQSISFEEVYDLAALRIITDIKM
                     NCYALLGVIHSLWRPLPGRFKDYIAIPKSNMYQSLHTTVVGPQGEHVEFQIRTEEMHR
                     VSEQGIAAHWKYKEHGRIDEKDGKVFSWLRQFVEWNQDLPDNRQFMDSVKLDLFHDVV
                     YVFTPQGMVKELPKGSTPVDFAYSVHTEIGDHCVGAKVNGKIVPLKHMMESGDMVEIL
                     TAANQTPHRDWLKFVRTSRAKTKIKHWIKAEEQSRSIEIGKRLLEAEFRRHGLAPAQM
                     MRSEQLLAVAKQVGYETPEELAAAVGFGHVPTSQVMSKINPPVATEGQTVAPEVPPPP
                     KPVTGRTDDTGVQVKGARDLLMQLSRCCNPVPGDRILGYITRGRGLTIHTVDCPNLEA
                     LDYDKNRLVEVEWDHSTPSTHSVKVSVIAVDKTGVLAHVSTAISECQANISRAEITTR
                     EDRKAMLDFIIEVLDTAHLARVLKAIERVDGIITVRRVRSWQERS"
     gene            complement(6178..7884)
                     /gene="recJ"
                     /locus_tag="PK224_08455"
     CDS             complement(6178..7884)
                     /gene="recJ"
                     /locus_tag="PK224_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRB15808.1"
                     /translation="MQEKLWVFRPSDIALQNNLSRQLSISPVTASVLLARGVTTPDEA
                     TRWMSGVHGGLHDPFLLPDMEPAVERLHQALARQERVCFYGDYDVDGVSATSLYLSFY
                     QGLGGNACGYIPHRVREGYGLNEGAVRRLAEEGILLLVTSDCGTTSHHEIAVARELGM
                     DVIVTDHHQTDATMPAALAVLNPHRRDAIYPFKGLCSAGLAYKVVSAYQQRYGSGDVE
                     PDSCLDLVALATVADIVPLQDENRILVREGLTHITRGARAGIRALKQVAGIAKACTSE
                     TIGFRLGPRLNAAGRLDHAMLCVRLLTTESDQEAMQIAEQLEQLNRQRQQIEEGITGE
                     AATYLQQDEPAAAIVLGSRDWHLGVVGIVAARLVDRFHRPSIVMAIDGQGIGKGSART
                     VEGFDLYQGLSECKDLLEAYGGHPSAAGLTLKESRSEEFRRRFCEVVAQWAGSARTVP
                     TLHVDAEVKLTDVNFDLIQELESLHPFGAGNPEPTLAVRGLDVVDARVVGEKHLKLRV
                     RQGRSFIFDSIGFRMGSFEELGLRAGRPVDLAFSPERNHWNGYDRVQLRIKALRMSGE
                     VS"
     gene            complement(8093..8290)
                     /locus_tag="PK224_08460"
     CDS             complement(8093..8290)
                     /locus_tag="PK224_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HRB15809.1"
                     /translation="MEKQERRPESRKDSHQKEEVKPNPKVVESGKKMKEDIDKLVDEI
                     DDVLEKNAEEFVKNYVQKGGE"
     gene            complement(8454..10508)
                     /locus_tag="PK224_08465"
     CDS             complement(8454..10508)
                     /locus_tag="PK224_08465"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRB15810.1"
                     /translation="MSDSAIVTFALIAAIAGIAYGLYLAMWVFKLNAGNAKMQEIAKA
                     IQEGAGAYMNRQYKTVGMVAAVLFVVLWGAGAVSDKFGMLTAIGFLIGAGASALAGYV
                     GMIIAVRANVRTAQAAHNGMNAALTVAFRGGAVTGLLLIGLGLLAITSFYTLASQMAG
                     QEKAIHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAFTMFKGVSAPILYPLALGGVTIFAT
                     IIGILFVKVSPGGEIMPALYKGLFVAGGIAAVAFYPITSKIMEGVGGVSGISYYLAAL
                     MGLAVTLALVFITDYYTSKDYAPVKDIAKASETGHATNIIAGLAVGMQSTSAPVIVIG
                     AAILGSYWICGGAAAGGLYGVAVAAVSMLSMAGIVVAIDAFGPITDNAGGIAEMAHLG
                     KEVRDITDPLDAVGNTTKAVTKGYAIGSAALAAVVLFAEFAREVAKGSQASTFDLSNP
                     LVLVGLFLGGMLPFIFGALCMKAVGQAAGLVVEEVRRQFRTIKGIMEGTGKPEYGTCV
                     DIVTQAAIQKMMIPGLIPVVAPLLVGVILGPQALGGVLVGSIVTGLFVAISMTSGGGA
                     WDNAKKYIEEQGLKGTDTHKAAVTGDTVGDPYKDTAGPAINPMIKVINIVALLIVSLI
                     VK"
     gene            10871..11125
                     /locus_tag="PK224_08470"
     CDS             10871..11125
                     /locus_tag="PK224_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15811.1"
                     /translation="MGLAPEYILIDQQKFSKTKLALDNHVYKNCEIDDCDVYYSGGQY
                     ELIDTHITNSRLILNHPAKGIYSAIQIFKMKSPGSSIISE"
     gene            complement(11215..12453)
                     /locus_tag="PK224_08475"
     CDS             complement(11215..12453)
                     /locus_tag="PK224_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRB15812.1"
                     /translation="MKNRRGLLQGFVIGLVLLAVAITSYGVQQGLAQDAKAQATIEKA
                     FPSSSKCKRCHERVFEEWETSPLSRSIHTPTFRAALDAYLTSSAGKDKALCFRCHAPH
                     VREFADQAQLFVTQAQSGEPSLDGVACVQCHLIKQVDRTKQPPEPKYDLGSKTMYGPY
                     KDFAQNLAHQSMELGLFHKSDLCLNCHQVVPAAADLGKSNDLLGNWDQSKAVKSGKEC
                     QTCHMPEQVGESANGEAKRKVANHTFPGRIGQLRQEAAKLEVSTKVEGEKTTVTVAVQ
                     SLVPHNLPTTHPGWASVVLELDIKGKNLKTVFNDKRVYGRTYADAKGQKTVFDFEAIK
                     VLEETVLKPEEKRVETFTFTTPKDTKTFDVEAMLSYAPVTGPSAFLQRIEAESSKGAQ
                     DPVFQSIPIAKFSENIPVAR"
     gene            complement(12522..13829)
                     /locus_tag="PK224_08480"
     CDS             complement(12522..13829)
                     /locus_tag="PK224_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRB15813.1"
                     /translation="MNDKGRSIQKLAMGGVLLFMALLNITFHERAIAQKTDGQVPADW
                     VAEIEKIFIRSEDCKQCHDRHYEEWKGMREQTPDLKSFGRVDAALLHGTSFESPVFRT
                     VLGVWMQTNPTMQERQRCLSCHAPAVTVFPQHVEKISAQILSGKPQVEGIGCASCHLI
                     NAVETTPLPPPTYKVQPGEMLYGPYADPEENLVHPATQLPLFRGANFCTSCHFDKVKD
                     VTQKDLPGEILQGTICQDCHMEPSTGSSTSKRGAMTRAIGRHWFRGVVIPGTLLKNRN
                     LQAEWMPRIEIDATKSGAGVEGTVLVKVGSLPHSFPDGDPVLKQFFLAITIKDAQGNV
                     LGEDTKRFGLPYDKILRGPIPDPFIKGGNTRRVPFSQTLKDGAAPATIEVVLSYALIP
                     EPEAELKAKYLATLANDQERATAKKVIEEYIQPRMLTYRAKSL"
     gene            complement(13854..14327)
                     /locus_tag="PK224_08485"
     CDS             complement(13854..14327)
                     /locus_tag="PK224_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRB15814.1"
                     /translation="MSKKNRTIFTRALSLGRALCVCGALFTVMGWGFQADAGTGMNGE
                     MTAEEAVQLGEEFGIAVGEVDEEVQKELKLQRPEGVAVFEVIGNSRADYAGIKVRSVI
                     KEIDKKEVRNLIDFGKAVKAAMKECNFTVGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            complement(14411..15244)
                     /locus_tag="PK224_08490"
     CDS             complement(14411..15244)
                     /locus_tag="PK224_08490"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRB15815.1"
                     /translation="MTSVHTSRTRLLTLLSSLFVLALILGGLGIPIVSSEGMIIRAQA
                     LSGDIPTAPEDPLWQKISPMTLPLSGQVITRPVWPEPTAHALAVRAVHNGTDIAFLLE
                     WQDNTKNDRLTPGTFRDGVALGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDRRA
                     SRTTEKDKAAIGEPRRFEVIPRRASSVEDLIGGGFSTLTTKEKQGRVQGKATWKDGTW
                     RVVMRRPLSSEEQENEAKLIPGRIQAVSFAVWNGENKERNGQKAIAPWFQLALDPATK
                     T"
     gene            complement(15329..16582)
                     /locus_tag="PK224_08495"
     CDS             complement(15329..16582)
                     /locus_tag="PK224_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15816.1"
                     /translation="METDVQLAVGYIETFAGNGKARSTGDGKRAVKAGIPLPHHVALD
                     RDERWLYFAESGSDRVRRVNLAEGTVHNFAGIGETCYSGDEGPCGEAGLYLPLDVACD
                     SRNDLYVCDSGSNRIRKIDRETGIITTVVGTGEHGFNGDGPALEVNLTWPAAIAFDAD
                     DVMYIADTQAHRIRRYDSRTGLVETIAGSWTAEDEAREQPLVARNLVVLSGDAIGIDF
                     SDDNGWLMPVCSDGLSLSMYLDDGHPAMEARLYDIVGIAVDNAGDVYVVDKGSNRVRK
                     IDHKTGLISTLAGVCRYGYDGDGKPAAKSMLHAPEAIVFDQHNHAYISDTGNHRVRKV
                     DADTGFISTVAGNGDSGYDDKNMGGCGAARFVAKDAAGALKHGDGLLAVEAVVNSPVG
                     LTLDTQGYLYICERGENKIRRAKLW"
     gene            complement(16629..16922)
                     /gene="tatA"
                     /locus_tag="PK224_08500"
     CDS             complement(16629..16922)
                     /gene="tatA"
                     /locus_tag="PK224_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB15817.1"
                     /translation="MFGSLGFTELILILFIVLIIFGAGKLPQLGEGIGKAIKGFKKSV
                     HEADAIEAEAQAQAQAQQAEPVQAQAIQAPPPVAMSTPVVEQPVAAAPPAARA"
     gene            complement(16947..17759)
                     /locus_tag="PK224_08505"
     CDS             complement(16947..17759)
                     /locus_tag="PK224_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB15818.1"
                     /translation="MDEQTQASGAEQGSAAVDPGDQPLSPDEEIAEIEKLLGAEPDDF
                     QARCRLGELYFSKGRLDDALTEVKKSIEMAEGLRTEMNRSLAMYYSNLGTIYATKGMI
                     DEAEAEFKRALDVHAYDVLALFNLGRVQADKRKYMESKNYYERLVEITPDDPMAWYNL
                     AGVYVELDNPQVSDYNTMDMAIQCYLRVLELDPKHLEASFKLMEIALNHRKTDLAIKV
                     MESAVEHSPDEPLAYYNLISVYDKCKMFEQAEQARQRLKERFAKKAKEGAAS"
     gene            complement(17793..18914)
                     /locus_tag="PK224_08510"
     CDS             complement(17793..18914)
                     /locus_tag="PK224_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15819.1"
                     /translation="MSAVATAVERSTGRVEDQGLLFEYTIKLVLFVGFFELVLYRLVS
                     RLGMHISKIAAQHEWVGTIFQLLTSVGFALLNVVAIFVFLALVVLLVNRARAKGITGF
                     NVVTIPSVALLLILTVSFLIVPPAMLGSIAYNVVTLIALTALMLEYLSQQQEWSKRVM
                     GAVYYFGISGWLYYQIFSTAYGWMGVIAAPPFVYEANRLGEALMVLASILVFWAYGRG
                     VSFRTRNRQQRRRAIWFSAVSAVVFFGLLFMDYFLGLYNPVLAHSIRKAGEGISWIFQ
                     MGMGYTFYLPFAFYVAGLLCWSYTVIRLVTMGRLAGYGLALMFIAGYALLFSSLTLMV
                     VLGVMLMTLDRPKGAVTEQAAVTRPPLVGTQESLVGGQI"
     gene            complement(19004..20785)
                     /locus_tag="PK224_08515"
     CDS             complement(19004..20785)
                     /locus_tag="PK224_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB15820.1"
                     /translation="MKVSLLFPPTWHPSQPYLSLPSLTGFLTQAGVKNVSQRDLGIEL
                     LDKVLTQSFAHGLYQQLVDKQQGLERERIGERGPGSAEQLARVIESLDRFPYLFERIE
                     LAKETLRGEGFYDIEAYRNSLFLIDKWLEVLSSLYFPTRMTVVDNQFGDYSIYSSKDL
                     IKAIRDEGQNPYISLFREHVLPSLLTDRPDLVGVSITATSQIIPGLTLCRLIKEHAPE
                     LHVTVGGSIFTRLVDNLRRCPWLFDLVDDFVVFEGETALLELVNQMDGKRDFSKVPNL
                     IYRQNGKITVNQPFYSENINQLTAPNYDGFPLDLYLSPEPVLPVQFSRGCYYKDCAFC
                     ALTLDHQNFRQRDPGKTVDDLVWLKERYGAQSFFFTDECFALSPTKRLCQQMIDRQVN
                     VKWTCELRFEKNLTRELLGQMRDAGCLKIVFGLESFNQRVMDFMKKGIKQESVRRIVD
                     DCVDLGIAVHCYVIVGFPTEKEEEALDTMNFVVGNKKLHESYGFSCQPCLFDLEKEAP
                     IMSDPGSYGIRRIMRPATEDLSLGFFYEVQEGMTPAQAEQLYQQVYEKISEVVCELPF
                     NYSMADGLLYIARAKSQALQVPQPTGS"
     gene            complement(21061..22014)
                     /locus_tag="PK224_08520"
     CDS             complement(21061..22014)
                     /locus_tag="PK224_08520"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRB15821.1"
                     /translation="MRLVQTRNKRLVFGILFSALIVGIMLTIGQVPLAVSQPVTIPVK
                     AIKGVIPMDGANPIWEGVPGVIIPLSGQTITTPMHPNISVKSVFVKAVSNGKDLGLRL
                     DWSDQTKNDTAIGPQDFRDQAAVMFPVNTAGAPPFQCMGQSGGTVNIWRWNAEWQKDL
                     GKDSAGMWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNSGIWSGNIMSDPTL
                     RVSSVEDLNANGFSTLTTQAHQDVIGNGVWEPSGSLKGGGYTGPTWRVVVKRSLETSD
                     ANDTQFKAGASVPIAFAIWDGSNIERNGMKALSTWFTLKMP"
     gene            complement(22114..23091)
                     /locus_tag="PK224_08525"
     CDS             complement(22114..23091)
                     /locus_tag="PK224_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HRB15822.1"
                     /translation="MTSKQVVQPTSPSSTAIAPPQAVAIKDSPAVERALNRSKIYLLF
                     SWSLLYPEDEEFLDYLQCGEFVEDGRAALDGLRLALDGIGGDRASQKIALMKKQFDQI
                     EKLVSAECVNWQIGDLQTEHRRVFTNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFY
                     KAFGVELSKDVHERLDHLSVELEFMHFLTYKESYSRCHDGIDKTEIVVDAQKKFIKNH
                     IGRWVPLFCRMLAKKSDTGLFKLIADCMSEWMDFEVAFLGVTVQPYSEADYRPATFNA
                     PEGQTYECGAQDKGNELSMLLSEVGAESFMDQQTKEKGGEKSEGPVGTA"
     gene            complement(23302..23907)
                     /locus_tag="PK224_08530"
     CDS             complement(23302..23907)
                     /locus_tag="PK224_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRB15823.1"
                     /translation="MAADPKYGRRDFLKDSVVSVAKAAREFAAHKDAPREQPAAPLRT
                     DWLRPPGAVNEAMFLERCTRCSDCIKVCPPGAIVSDVANGTPVIFSNQVACELCDDFP
                     CIAACATEALLPVADCFDVRMGVATVSHRVCTAGQGCHACVSKCPVEALSMDFHALHL
                     VVAPERCVGCGMCEQICKTVNDRIAIKVTPARNLSAGALGY"
     gene            complement(23920..24951)
                     /locus_tag="PK224_08535"
     CDS             complement(23920..24951)
                     /locus_tag="PK224_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15824.1"
                     /translation="MATGVEVSKPSMEIRFQPALLQEVIDSFIEKTEREGDPTYYKEF
                     HELADPIYEKFTLDDREAEFKKLYQYLFGIWGFSDIIRDAFNEFPELKQRVGIVLVKG
                     VLKEDQEGVDVLRKWGSVEHDLAKQFEEKGLKGVGIKLIPRRFYDPALTRYCRHELLH
                     IHDMLDPAFGYDPDTRVGQNPGEETLILHRYRILWNISVDSRLIASGKEPMLQKEDRF
                     KEFRSWYRKIPPAQLKSVFEGLWQTSYFTHSELVEMATDTLRILDRAVDVEGGEIPET
                     TNKVMLMPGFPCPLCRFPTYTWVEDMETKLEKYILDFIRENHPGWDVEYGACDRCVEV
                     YKLRADGVM"
     gene            25150..26259
                     /locus_tag="PK224_08540"
     CDS             25150..26259
                     /locus_tag="PK224_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRB15825.1"
                     /translation="MSNQTTAPLTAPSTGQPSSQSGTAQPPPPVDHLEYWKAGLRELK
                     TFRGHSHGVWSVAYAPDGQTIVSGGVDRYVRVWDIETGRLLRSLRGHTADIRALVFTP
                     DGRTLASGSEDRTIRLWNPKTGEPTKLLFTRYDHNVCSLSLSPDGLMLARGSHNKDIK
                     IWEVTTGTDLMTLLGKDQYDHHWSVCVAFSPDGVHLASGSDIGKIRIWEVLPSGEEKI
                     LHNGHWEETAEDSTETRGFFIEDDGGFQKPMEYWIGAMVFTPDAKMLITGSRDNTIRF
                     FEMPTMNELRVVRGHNGWVRSLVVSPDGKVLISAGDDNTIRFWDIATGRNFRTDKTHG
                     GAVRGIALSPDGLRLASASWDRTVKLWEGGVEPAE"
     gene            complement(26261..27712)
                     /locus_tag="PK224_08545"
     CDS             complement(26261..27712)
                     /locus_tag="PK224_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB15826.1"
                     /translation="MKGLKVLIVDDEPLMRLSMLDALEGIGCEVMAAATGTEGLTILG
                     TRQFDIVITDLRLPGADGLTILKACKERSPTTEVILITAHGSVDTAVGAIKLGAYDYI
                     TKPFQMDELLLIVERVGKILGLRRENLELKEALEDRFSFGGILGCNHQMRALLEKIKL
                     VAATDSPVSIVGERGTGKELVAHAIHLNSPRRDQPLIKVCCADLPEALLEAELFGHEK
                     GAFPGALRQRRGRFELAHKGTLLIDDIDAVSPNVQARVWRVLQNRRFERVGGREHIEV
                     DVRIVCASKDDLKGMAYQGRFHKELSDQLTAVQIVVPPLRERREDVLVIAEHMLEARS
                     AALGKSLDGFAQPSRDLLLHYSFPGNVGELEQMVDRAAALGRDGESLQPWDLCGFQTC
                     PYLGGSPQAGCGFCSEGLTAMGEKPESTAPATLATAREAFERDYIAAVLKQVDGSRTN
                     AATILGVSRKALWDKCKRYGLSSAKGEAEEKDE"
     gene            complement(27709..30885)
                     /locus_tag="PK224_08550"
     CDS             complement(27709..30885)
                     /locus_tag="PK224_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB15827.1"
                     /translation="MLFWSRSHSLQRKIITAIVMVGLLPLCLSLVLTYIEERRALRET
                     IGANFKEVAVEAARRIEMQVTRGVNEAQQLAATPFLRTSVTESNRTYLDKDEKTVQSM
                     IKAWQQRWRQRDKQSEFPLFINRIVTNYLIRWHDIRKADYVGIFVTDNRGALVVSSIP
                     QVEYYHGKAAWWQAVMKRGTGKVFVSDIFFDPAFGTHVVNVSAPIIDDEQHVTIGAVT
                     VLLRRDTLFHSVSEATVGQTGHAMLFNSDGVPLICPVLAPEEHAVKPELVSAISASPA
                     GWTTLANDSHGGQNSIVGFAPVRLGTELTPDSLGGKRWVTFVRQDPAESYAPLSRLVV
                     QVTVYGLGVFGVLLLTGLTVARRIARPIGLLHEGVQRIGSGRLDQQLDLKTGDEIEQL
                     ADAFNKMAANLRTSFEQIEQRMEDVRRLEARYRDLIEHSPEMIYQLNKAGQFVHVNKT
                     GLDKLGYSLEEMLQMRLWDLVPKGREAEVLAYLERLVSQGRSTIETVFVAGDGHPIDV
                     EIHATALFESGGGLVHSRAFVRDVTERRLLEAQIHQYTTRLEQAVNERTRQLVASQER
                     YKALFDLVADSVFMVGEDGVIVAVNKREEQALGYSEQQVVGSSILNVVEPVHHDEMRV
                     LLEKIRAGERQVPTQEITVRDVTGRETPVEMDLILVRGSDHTWVMVQLRDITDRKRLE
                     RQMHTYQQELEAKVSERTREIEETKQYLENLLENANDVIYTLDSEQCFTYVNSKVLAW
                     GYAKEDLLGRPYLGLLSKRHRGRRLKSTLDIGVKQVYEVEVLTKAGEPRSVMVSVSPL
                     HGVEGEILGVLGIARDMTDTKKLEQQIRNSEKLASVGKLAAGVAHEINNPLGGILNCL
                     YNMRKGTLSPSRQEEYLASMEDGLRRVQRIVRQLLDFSQQHNPELALADINQVVNRVL
                     LLTDHLFVPHRVQLETALSPDIPELMIDRHMMEQVLMNLVLNAIQAMRTGGVLTIRTS
                     MDEAHCLVRIQDSGCGISSSVLPRIFDPFFTTKNEGEGTGLGLSVSLGIVERHGGRIL
                     VESEVGKGTTFTVSIPLATERHAIGRFS"
     gene            complement(30961..31878)
                     /gene="secF"
                     /locus_tag="PK224_08555"
     CDS             complement(30961..31878)
                     /gene="secF"
                     /locus_tag="PK224_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRB15828.1"
                     /translation="MFEILGKTNIDFMGKRSITFAISGILAFLGLIAVLQIARGAANL
                     GIDFAGGTAVQLKFEQAMKIDEARRALENNGLGSAELQEFTQDNKLLIRVKASTTIEE
                     KVAERVMAVFSKEFPANKFVVDSSMEIGPTIGKKLQEDAMIAVLISFVGIVLYIAVRF
                     ELRFGVAAALATFHDVLAVLGVFYLLDKEITLLVITALLTLAGYSLTDTVVVFDRIRE
                     NLRTRRREDEETIINQAINQVLSRTIVTSLTVVIVLIPLVLAGAEVLHDFSLALLGGV
                     MFGTYSSVFVASPLLLLWPGSAGGLMKRR"
     gene            complement(32094..33743)
                     /gene="secD"
                     /locus_tag="PK224_08560"
     CDS             complement(32094..33743)
                     /gene="secD"
                     /locus_tag="PK224_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRB15829.1"
                     /translation="MKKVGGRLLALVTMVVASIIFFLPSYQPLYKELPRWVREVLPDK
                     GITLGLDLQGGIHLVLEVEEDRAVEIAVERTAAGLQDLLVEKKVPAESVKRTGSSQIT
                     IGFQNAELKAQVQKLLDDFPTYVEVESAGSANKVVWELRDAEIKRIKDSAINQALETI
                     RNRIDQFGVAEPLIQRQGLKQVVVQLPGIKDAKLAKDLIKQTALLEFKLLDDENQLKL
                     DLPGRIQKGKEREDAFLKQVEGKVPEGDQILFERIVEKDSGQEWLAPYLVKKRVMLAG
                     DVLSDARVSIGQFNEPYVSVTFDAKGAREFDRITGENVKKRMAIVLDNTIYSAPVIQE
                     RISGGRAQITGTFSMEEANNLSIVLRAGALPAPLKIIQDLTVGPSLGQDSIEKGVKAT
                     LFAGLLVIVFMALYYRLSGVIANFALLLNLICLIGALAGLNATLTLPGIAGIILTIGM
                     GVDSNVLIFERIREELRQGKAVRVAIDAGYDKALLTIVDSHVTTLITGFALFLFGTGP
                     IKGFAVTLCLGIAINLFTALVGTKVVFDLFNQRRKIEQLSI"
     gene            complement(33779..34099)
                     /gene="yajC"
                     /locus_tag="PK224_08565"
     CDS             complement(33779..34099)
                     /gene="yajC"
                     /locus_tag="PK224_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRB15830.1"
                     /translation="MWDSVAWAQGTSGSTGAGGGLLSLVPFVLIFVIFYFMLILPQQK
                     RRKQADTMLAALKKGDKVVTASGIWGTITNLGKDTVTLQIADTTKIKIQKEHIARLRG
                     EEED"
     gene            complement(34123..35280)
                     /gene="tgt"
                     /locus_tag="PK224_08570"
     CDS             complement(34123..35280)
                     /gene="tgt"
                     /locus_tag="PK224_08570"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRB15831.1"
                     /translation="MLTFRITHEESGGRARVGVLTTARSEIATPAFMPVGSLGNVRSV
                     DGEELLKMGYGLILNNAYHLYLRPGHKVVEELGGVHGFTAWPGAILTDSGGFQVFSLA
                     KFCKISDEGVMFQSHIDGSSRFISPETSIEIQEALGADIIMAFDHVVALPSSLDQVRD
                     ASKRTSLWARRCVSAKRRTDQSLFGIVQGGLDAGLRVESARDLVSVGFDGYAVGGLSV
                     GEDKADMYGMLDVTVPELPSAKPRYLMGVGMPEDLVEGVARGIDMFDCVVPSRHGRTG
                     WLFTSFGRVVIKQARYARDEQPIDPACRCPVCTRYTRAYLHHLFGVKEMLGARLNTIH
                     NLWFFAKLMEEIRSAVRGGTFQEFRREFHRTYVVDRPADDGRSDTQSTNGS"
     gene            complement(35294..37048)
                     /gene="argS"
                     /locus_tag="PK224_08575"
     CDS             complement(35294..37048)
                     /gene="argS"
                     /locus_tag="PK224_08575"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRB15832.1"
                     /translation="MTQGVVQDKVSQALVGALRSAQQRGVLKLEQMPMVNLEAPKRPE
                     WGDVSCTVAMSLSASERRPPFEIAQIIIDHIEQRDALFSRAEIARPGFLNLTVKRELW
                     MDVLREIEAQGERYGHSQVGQGRRVLVEYVSANPTGPLHVGHGRGAAVGQALVRLLCA
                     TGHDVVSEYYINDAGRQMKLLGLSVLARYREACGQTVSFPEDGYHGGYIRTVAEHVKA
                     EQGAALLSLPAADAEQRSREFAYRELLALIRQDLEMFGIAFESWFSEASLLSSGAVDQ
                     VLSELRARDLLFDQEGAQWFRSSAFGDEKDRVVRKQEGDYTYLASDIAYHRDKLRRGF
                     DLLVDVWGADHHGYIPRMQAVVQAYGHPKERLRVVLVQMVNLLRGGQKVEMSKRSGEF
                     ITLREVMDEVGADAAKFFFLMRDSSTHLDFDLELAKQQSQENPVYYVQYVHARIASLR
                     RVAASRGIACPLPSQADLNVLEDPDELALIRKLSAFPVVLQASAVELEPHRMAYYLRE
                     LAGMVHPFYNKHRILPPAPDSEAAAALSTEQSGSSGRATEVMTPGLTGARLALMWAVQ
                     QVVRNGLNVLGVSAPEQM"
     gene            complement(37133..38014)
                     /locus_tag="PK224_08580"
     CDS             complement(37133..38014)
                     /locus_tag="PK224_08580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRB15833.1"
                     /translation="MGALLCGAGEVDRVFPLESSYLSGLWAGPDSLCSEFRAWLGNCD
                     IAVGWLPDAEGALSATLRASGVQYVCLQSAASTDLCAEHQASRYLDAIQLSGTSQLVG
                     TPLVLSPAIRERSRLVLQALIQENRHTVVVIHPGSGSPHKCLDARLLVPVIEWLLQAD
                     MAPLLLEGPSDREAVERVLTTIKVDVPVIRGLALSTMAGVLSHAKLYVGHDSGVTHLA
                     AALSVPTIACFGPTDSRRWAPLGRAVSIVSGAPCTCRTWSAVAECRERVCLQIAPERI
                     IEVCRLLLSERRTVPAT"
     gene            complement(38164..38784)
                     /locus_tag="PK224_08585"
     CDS             complement(38164..38784)
                     /locus_tag="PK224_08585"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRB15834.1"
                     /translation="MSERTLVDVTGVLIAGGKSLRMGRDKRFLKVNGESVFDRTLSLL
                     MDTFVETVVVLAEPIESLEVRGCRVVYDVIPNAGSLGGLYTGLLAASQPRIFAVACDM
                     PFLHAEVIRYMVSCDETADVVVAELEGQFQPMHAVYSKRCAEFLKAMAERQNLKIQTL
                     YRSEALRVAVLGANALSSLGVGLRSFQNINTPEDLALANAPISDKP"
     gene            complement(38877..40661)
                     /locus_tag="PK224_08590"
     CDS             complement(38877..40661)
                     /locus_tag="PK224_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB15835.1"
                     /translation="MSKLRMNVKATPIIGAALGALILSASAVGLGYAGDLPEGFAKGE
                     LAPAPPAEMIEAGKRVYFTKCVWCHGVDGAGDGPGADRLWPRPRNFNQGTFKIRHTAS
                     GELPLFNYNEKTPDSGKNDLLDTVTHGLPGSAMPPWEGILTEEQRLQVLSFVTTQLVK
                     DRKFTDKQSETQTILQLGTIKPAEPTEESIKKGAQLIVDKKCIECHGVEGRGDGNAFN
                     LKDDWGFSIQPADWHKCWNFRGSRQDPYNVRNIFRTFSTGVNGTPMPSFADSTSVEDR
                     WSIANFVNSLCERDAEGKPLGIDPLTDKPKINFVVPSAPVEGEISNDPENEMWKKQGR
                     RYVAMGGQITHKPRNFVNRIDDIWVKSLYNEKNVVYLIQWDDRTKSVAEGKLPWAPTQ
                     VNVENFGVKEQAPKTGEEGSIAAAQNNYAVYNDGIAFQFPIKWQEIPAPFKPRYLWGD
                     AKFNADILKWEADGSLRSFKGTGWDQDFEERDDFEEKVKLLKSEWKNGQWTVMISRPL
                     KGDKDDYDEYTRFDIGKYIPMVFFAWDGHNGDAGRKMAVSAFYYTILQPPIPQEVYIY
                     PAVIAVGVVFLEGWMLTRRANKKKGKVL"
     gene            complement(40709..41632)
                     /locus_tag="PK224_08595"
     CDS             complement(40709..41632)
                     /locus_tag="PK224_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB15836.1"
                     /translation="MPMQVLSELAIFVAGFTGLSYLANSMGIAFPRFWQGVLFWAFIQ
                     AYLKFRIYPPIPFSVRAMYGTVSFVAVFMWVSANEEDWKKFRQPILNVLDANTGFHKA
                     LRTMYLILLPILIGGFSFMTMKPSVDEPIELRTVHPAPPASTKVHGKTYTLQTSQNPY
                     RVNLEGKYDQAYSNKLIVEQGMGRLMAPNANPWDDKAEGYLKYVREGGEIFFQNCHFC
                     HGDNLNGRGLHAFAFNPIPANFTDPGTIAQLQETFIFWRVAKGGIGLPNEGFPWASVM
                     PPWEQHLTTDEIWKVILFEYWHTGYYPRTWD"
     gene            complement(41795..43111)
                     /locus_tag="PK224_08600"
     CDS             complement(41795..43111)
                     /locus_tag="PK224_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HRB15837.1"
                     /translation="MIELIKAAVAMGWPALGILVALMFYFKASISDPVANKRAVFKTF
                     IGIIGAMLLFMAIANYSMNFFAESRLLPVSLVLITSMTFMMALYFTNISALLKIGGFM
                     FFIAAALSGYGNWLPQVEGGFPPIEEKKDFSNMPQTELADEGEKIIFGGVGQNKVQGA
                     IGKGQCPLCHAFHKGMLGERAPNLDGLPERAGTQIEDPRYHKGNAAARDSDQKEAFPG
                     SGTAENAQEYIAESHACPSCFVVAGYGVKGTNDKVSPMPSIHKPPISLSLPELAAVDT
                     WLYVREGRDAPSFDEIVKSYEKFIPESERPKPPTEGDKAAGGGAALMADGTEPVDQIF
                     AKGQCVACHTIPGIAGATGTIGPKLVEGTNAPLRIKDKDYKGKAKSVPDYIMESIVEP
                     SAYVVKGFPDNTMPKVFGQKLSAGALKKLVDYLSQVQEGKEPPKAS"
     gene            complement(43200..45119)
                     /locus_tag="PK224_08605"
     CDS             complement(43200..45119)
                     /locus_tag="PK224_08605"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB15838.1"
                     /translation="MGSVTRTKLMSIMALGAMVGLLLLPILVAIPALAGDGGAPAAGV
                     KKEGGDAKVEKGKDVYYKTEGIVSGPPAPKTTDSEKDYPRYNFESRVLLWFANQQHLY
                     YGSFVLAVPIFCMVIEFMGVVTKDKAMAKRYDQLAYDFIKISLTAYSLTAILGGILIF
                     TFLTLYPAFFQYLSGIFRPVMHIYALMFVAESGTLYIYYYGWDKMKEGFLKWIHLSMS
                     VVLNVIGTILMFLANSWIGFMMSPAGVDEQGRYLGNIWHVIHTALWNPLNLHRILGNM
                     AFGGGVVAAYAAYRFLSAKTDEERAHYDWMGYIAMALGVAFLIPLPFAGYWLMREVYA
                     YRQQMGITLMGGLLAWLFIIQATMIGILFLSTNYYLWQALGRMRGAEKYQRYIKYLVF
                     LLTCGFLVFITPHTIVMTPAELKAMGGQQHPVLGNYGVMSAKNGGINVIITTTVLSFV
                     WYMRGNKVSTVSWSKFGNIFMGCFFFFAYLNIIMLAVYGYYIPANVRVGLSVPQVATT
                     LSCLFFMFALNSVMMKGAKQMGPIEWGKISARSQYALIMLATAFTWMMGLMGYIRSSV
                     RLFWHVNEIMRDNSPWAYTHTVGFAANMISANVLFFWISIMFVFWLGALAAKKAPVEA
                     KAAVPGRAAAPAVGH"
     gene            complement(45184..45306)
                     /locus_tag="PK224_08610"
     CDS             complement(45184..45306)
                     /locus_tag="PK224_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15839.1"
                     /translation="MGQTIVYVVVSVVICVSFFAAVDHFLMDAQGLDFWYLLRK"
     gene            complement(45634..46587)
                     /locus_tag="PK224_08615"
     CDS             complement(45634..46587)
                     /locus_tag="PK224_08615"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB15840.1"
                     /translation="MLETRFKVIFLLVVLFAASLPIIAILRGTISTPFEAEPQHAEQD
                     QEVSNPESASAEEPLEEELVAIPAGPFIFGTNRGGFDEQPERTIYLDRFLIDRYEVTN
                     AQYAAFVKATGHRKSGPPSRYAKNTSRMRGVNQPAVYVSWEDAKAYCEWRGRRLPSEA
                     EWEKAMRGPDGRLWPWGNVEVPNAANWARVDDGFEVAAPVGRVKSDTSPYGVMDGAGN
                     VMEWVNDWYLEGAYAAASDRNPESPEYGTYKVLRGAAYTSSGSDLRITARSKMMLDFR
                     DETIGFRCAQSSAENGQPSKGMGSDKIIGNRSSRGSETQPK"
     gene            complement(46599..47513)
                     /locus_tag="PK224_08620"
     CDS             complement(46599..47513)
                     /locus_tag="PK224_08620"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRB15841.1"
                     /translation="MENRGVLIGSIIFVFGSFILMIGGLVYESYKAKQLRQQVLSITT
                     EVKPVAVAAAQDFSMYKTLVGDDGRDMVQVSEGPFVMGSRDNDSDPDEKPEHQVYLKA
                     YFLDRHEVTQDAYDRFAKMTKRIKRKIEVFEEDPAKLLKPEYPMIAVTWDDAEAYCKW
                     AGKRLPTEAEWEKAARGEGKRRYAWGDEFVVGYANIDGNEDGFRYLAPPGSFESGRSP
                     YGIYDMTGNVGEWVADFYDENSYRGSPYRDPKGPDQGEQRVIRGGSWRETKRNVRSSK
                     RFQAKPWRHDITVGFRCAKDIEADVVVK"
     gene            complement(47529..47930)
                     /locus_tag="PK224_08625"
     CDS             complement(47529..47930)
                     /locus_tag="PK224_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15842.1"
                     /translation="MAGMNPPSTRVLLEVAFGAVVMGTVGALIGAILGGSSIPLASGL
                     GLVLGAVVGFLGGRRFLVSILVGTVLGGLLAWFIAGIEKVSFGAGAGAAMGGFLGVQI
                     SMLLDVRAARKAAALEASSDEASADSAVIKS"
     gene            complement(47965..48624)
                     /locus_tag="PK224_08630"
     CDS             complement(47965..48624)
                     /locus_tag="PK224_08630"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HRB15843.1"
                     /translation="MATKMFAAIGLLGVVGLCSVMGVGAPALAGEAGVLVLEDFQSAD
                     QDGFPIDWQHENQRSQAKGRDAYKIQSQDGKKFLAAKDAGQRVKKRKIDWDPKVYPVL
                     TWRWRLHKAPSGSEPIAALYASLDTDLMFIPVFTKYIWSAGKPEGTFVEGGMFSGSEL
                     VVQSGVLPVGEWVEERVNVYEDFKRIHKHEPAEKAWGISLFAGPGVEIDFGTVTVSSG
                     K"
     gene            complement(48648..50516)
                     /locus_tag="PK224_08635"
     CDS             complement(48648..50516)
                     /locus_tag="PK224_08635"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB15844.1"
                     /translation="MIKRLPLPSVILLLLSGLLVAASVLLLSPPSLVAADPSADVYFH
                     TPGVPDGPSAPTANENHYPQVGSLDSRLLMWFIIQQHTYFGGFVLALPIFCVLLEFLG
                     LVARNPAMAVRYDGLAQDLLKVALLALSVTAAVGSVMLTMFITLYPSFMQYMGGTFKV
                     MMPLYALVFVGTTFLTIAYYYSWDRMAAPGSKWVHLSIGVLAVVFGTSLLLLANAWSA
                     FMMAPSGVDGQGRYLGNPWHLLRSALWNPLNLHRFLADIMSGGAVVLAYACYRFFSGK
                     SQEERAYYDWVGYVFLFVTVCALLPMPFAGYWLMRSVYAYSQSMGVTMMGGLLTWLFV
                     VQALLIGALFLGVNYYLWQSMGRIRGGERYQPYYQYLLLALTGCLFIWLTPHTILMTG
                     TEVKAMGGAQHPVIGNYGVMSSKNGAVNVMICITALSYIFYRRANRTMTISWAKAGNV
                     ILGALFGFGTAHIIGLSVYGFYLPASLRVGLSGPQAVTTLLVVTLGLLLNRRMLRGAT
                     IHGPVQWGQISVRGMVGLFGLAAAFTWVMGLMGYIRSSGRLAWHVNELMPDTSPWAFT
                     PSLGFAAKMVTINMALFWGAVFFLFWVCQRGQQPVMHEDVSAEKEAGFLPSPSHEG"
     gene            complement(50513..51598)
                     /locus_tag="PK224_08640"
     CDS             complement(50513..51598)
                     /locus_tag="PK224_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15845.1"
                     /translation="MLHRLALRVLPSLTLAVTEDSVRTRKRLVMFVPGLVAFAVYRAA
                     KHGMSLAEPVTLLLLSGLVSLVTAICAYRVGRGVPFAQLVSADGARRIGWILAWIGFV
                     YGVQLSLLVLALLWLVGYDYAKHPDGPAMMAMIIPSTAVARDAFEIGYVRWLEASGRP
                     FVTFPDGAGLRALLARVTPALAGWVAAGVAGCVSLSLLLAALVSGDMAVLAQGAVVTM
                     AAGTVGLLAFFAGQGNGAAWTAGLSSTRWSELVKFWWWPGLAFASTYYLVLVGGCLYV
                     LKQPMSSPGLFAGVAGLVGGVMALYCYYLGHRRDIENRQPGGVPSALLRCPFVMGILG
                     KSREAIAGTALSGSAAVFDKNSQRASS"
     gene            complement(51675..52148)
                     /locus_tag="PK224_08645"
     CDS             complement(51675..52148)
                     /locus_tag="PK224_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HRB15846.1"
                     /translation="MHTEGVEERISREGDPTWFVRSECRPCQWVVGVDVPVGQVDGLV
                     DRLMWTDDARHRLDRVPPYAVPVLRELVEGFARTRRQRVITYELIDQAKTGDMVSWDP
                     DAEQRLANVPAPVRAMARVELERTAVDRGERSVTVALMEEVKARYFGMAAQRDDA"
     gene            complement(52211..52927)
                     /locus_tag="PK224_08650"
     CDS             complement(52211..52927)
                     /locus_tag="PK224_08650"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRB15847.1"
                     /translation="MFEFLRQPGFFGTHATMGADLSQLMATLFTGLFIIGWLQAKQHR
                     GHHHHWLMLGGMVTMVGFFTAYYLFRQLGVLAFEGKEGFGGSQALYDYVFIPVLTIHI
                     ILVIIGLVMAVYMIVLGFRSQQFLSGARVLSAARLLTSWKKVSLIFAALLAVVMLLFG
                     TRVMSAGFSMRKLEVYIGFLTLVAIVFAVEMGIQRIWPDGGQRHRALGRFTMIVYCIL
                     FLTGTFTYAMLYILYPGKIG"
     gene            complement(52936..53925)
                     /locus_tag="PK224_08655"
     CDS             complement(52936..53925)
                     /locus_tag="PK224_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB15848.1"
                     /translation="MPRELSLAEVFQLGYYWETKILLTAVKLDVFSVLDGRGRTASEA
                     AEKLGADVRALELLLNALVAIRLLSKTGDLYANTPVATTHLVKHGPQYIGHLLLLHDA
                     EWGNWGKLEEAVKTGRSPVTQHVFETDPALGANVLSVLHRIGQQSGPDLAKRLALGQA
                     RTMLDLGGGAGTNAIAFCQVYPQLSATVFDLQTTLPLTERTVKDAGLEGRISLKAGDF
                     NRDSLGGPYDVVLMSDILHYQDLATNAALVKKIYSHLNPGGRLIIKDRFLDASGTSPA
                     WTAAFAVHILVNTEQGACYRTAEAMQWMHDGGYVSVEEIERTAVVQGVRPSVG"
     gene            complement(54208..55005)
                     /locus_tag="PK224_08660"
     CDS             complement(54208..55005)
                     /locus_tag="PK224_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB15849.1"
                     /translation="MKKGAMKVVFGVAAAAFLAAPLASFAGGTIAGKVTYAGKAEQKE
                     FSFSKFPNPKFCPKNPNKSLMDGDKRFLKTIEVAKDGGLKGAVVSVVDIEDQAFQDGF
                     KGTDVTAEFCEFLPFSGVVVNNKNFRAENKDADPDDPKSTLGVLHNPHSFVVKGSSSA
                     TGFNIGLAKKGDKLEKPVVFRGGAEKQGYYRLQCDQHEFMQSFFLPVWNPYHAVVKDD
                     GSFELPGVPAGKHKVVVWHPFVGKGKLNEFEVEVAEGGTANLKAEIK"
     gene            complement(55218..56615)
                     /locus_tag="PK224_08665"
     CDS             complement(55218..56615)
                     /locus_tag="PK224_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB15850.1"
                     /translation="MALHNESRSLLTMSGWMAVAMATGVLLCLWPSAASARSLKEAQA
                     AFDKKQYQEALDLVEQVTKEQGPQPEARRLKIHSLILLAKPKDALVEYEHLERDLKQD
                     DRALLKEVALGFVYVLLKDMREQMRGAAYTALKDVDSPEMIPALEDGLSDGSGLVRAL
                     AAEALGKLEAGRRSPRLRNALEDQAGLVKATVLKVLGKSGDRSVIPLLEKALKDEQPA
                     VRLAAAGALYHTGQTAMWDTILKAASAPNPEERATALRMVGELKDPRGLSVLLEAITN
                     TQPSVRGAAASALGDLGKVQGIPALEHALEDKIPAVKTSAAISLGELGVKDSLVPLRK
                     ALADQNPVVKAAVVSALLRVEEPFESVGEELYELAQNHDPGTRSAAGKAAGRAHGANM
                     KAAIEFLSGMLKDPIPRPRIAAARALGQIGGAEILPMLKLALHDEDDAVRATVGGSIA
                     RILNHAKPSPNRAKS"
     gene            complement(56645..57250)
                     /locus_tag="PK224_08670"
     CDS             complement(56645..57250)
                     /locus_tag="PK224_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15851.1"
                     /translation="MGAGFSGVLCLSGFGYASAADGPSAIGPAEFSASLVKRVEGRRF
                     EAQVFAKGDRIRLEYKYAVKTELGYSSIEIIRLDKHESWFLLAQRRQILPVPVKPEEI
                     LPIQPSLPGEKSRTLVGDATTIGRASKLYEVRVDYNGRNERFYEWVDVETGVVLKLVS
                     QDRDWSIEYSRIRLSPQPDYYFEEPTGYRRWAPASATQERG"
     gene            complement(57282..58208)
                     /locus_tag="PK224_08675"
     CDS             complement(57282..58208)
                     /locus_tag="PK224_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRB15852.1"
                     /translation="MRLTRSSLLCCLALVVWLCQVVPSVSAYDIVDVQHGGSLEGRVT
                     LSGAVPDPKAFNLITFPDPTYCGRISNGKGWRLLRDFIVNAESGLKNAVVLLEGVESG
                     KPFDLSVPLIEARDCMFGPWMTIVRNGHAVEVVNMDPVMHDIQGYETSPEAGARVLFN
                     TPLILNQQHQRGNMHAIHNHAPGKSLVGPVYLNKGRRTFYMQCGFHAYMESWAMAVNN
                     PYYAVTDDQGAFRIENVPPGTYQMVVWHPQAGPGVTRTITVGPDGATVEQVALPAPRG
                     TRTAYRVMDNPRFGLESLGHPVEIEPLVEHQH"
     gene            complement(58238..59146)
                     /locus_tag="PK224_08680"
     CDS             complement(58238..59146)
                     /locus_tag="PK224_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB15853.1"
                     /translation="MRLVGLAVLCAVSVCWSPSWAYEEIAVTDGGTIKGTVTMTGGKP
                     TPKGYNLITFPDPVYCGRISTGTGWRILDEFSMASSQGLKDVVVVLTDVTKGKPFKFE
                     PLTIEARDCRFLPFVTVVKDGSEVAVMNMDPVMHDIQAYETSQLGPRVLFNTPLPMNP
                     HHKRFVSAESHEHLAGEPVTEVIHMTKGRRIFLMQCGFHAYMESWGFAVDNPYFALTG
                     DDGTFSLTDVPPGDYTLMVWHPGVGTMLQKKVTVTEKGLSNVDFDFKAPQGRRSVHEI
                     EQNPHYGPESLGKVVDIRPTLQRQVP"
     gene            complement(59305..>61911)
                     /locus_tag="PK224_08685"
     CDS             complement(59305..>61911)
                     /locus_tag="PK224_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15854.1"
                     /translation="KIGKEPPVIDPNGLIYVLHEEEAEIRKNEDLKYPLVVRGNIYDC
                     VDWTLTSEWDDDDYTNFQSSKINTHWHFLQFDNQASDGVITGFSYEQSVRPFTMLEKK
                     NAKGLPAPMNTVVTAAAKKGANTITVKNAKQFHVGTLLLVGADNVKGNEISRIKAING
                     NTITLAKGLKNDHPANDIVTVEFVRQRFWVDADVGTVFWHDHAFGATTWPHGGFGTFI
                     AEPVGSTYHDPKTGKLIRSGPIADIHTNEPVGHGVNNSFRELMVQVHDTVPHTVNIVT
                     AGNPPGQPIEVALEAGKTVSFMMPEKIYMTPMPFLNGGTHTTGSGLNFRAAPVAQRLA
                     TNPDSSQIFSSLVHGDPYTPTLRAYVGDTMVFRLLHTLMNESMVWTLSGHTFWTERYA
                     SDANRKNSIHIGIAERYDLVVPEAGGSRHQAGDYIHFNGRSSKFSEGGWGIIRVLDKE
                     QADLKKLPAGFSIKGEIPKPLPVCPADAPVKQFNVVAMDYAGMKFNAKAPESIEVDFE
                     RRILMTNAEAKIYALEEDTAKVAGGAHPMPLTLRVNSGDCVKVHLKNKMKDSKASFSA
                     IGLAFDPKDSMGANVGNNPGDQTVAPGAEREYTYYADPFNGETTSLVWDWGNVMTNPR
                     NGLFGAVVVGPKGSKYRDPKTGADLSNKNAWAADVIIDRSVPGNEMRANYRDVALFFQ
                     DEDNIIGTSFMPYVQNVAGLTGVNYRSEPYKFREEQGCSLGKVFQPCKADKPEDPATP
                     LIEAHAGDPVRIHVLGVSNEQNGMFSVEKHEWPIEPFMRGADLISVVEFSGSETIDAF
                     LPSAGGMYRLPGDYVYSNQRLPYSQSGQWGYVRVLPSGDTRLLPLAGASAGTKSAEVE
                     QPVAHAIPVAAK"
BASE COUNT        14055 a        19635 c        15637 g        12584 t
ORIGIN      
        1 aagtggcgcg tattcaggtc gaagagccga tctacattcg gccggccgag cgcgtgaact
       61 ggctgtaaga ctcacactga cgagagaggg caacttctct cgtcagtgaa ggaccgcagc
      121 acaacgaagg caggaagttc gaaagaactt cctgccttcg tactttcagg caagaagcag
      181 acattcacag agccggcgac tgcacctatt caggcacttc ccttacccag ctgaacaaag
      241 agcgccgaag cgcccacgat cacaacggct gagctgcata ccccacgaga acccccttct
      301 gcttagtacc cccccaagaa acggcccgat tcctcctcca gtaccagatt tcatcgtcac
      361 ggcgaagcct tggatacatt gcgtttccca atatctggtc gggccatagg aaacacgcag
      421 ctcatcccca tcatggaggg tatctgtgca gatgttgaca gctccccctt tgaccgctat
      481 actcaccata gttgagaaca gttatcagta tcgagaaatt gggtacgtca gacgcccgca
      541 aggacaaccg atcatcagtg ggagatgcca ctcatgtatg tctgcttatg taagggctta
      601 acggaatcag atgtgcgggc ggccggtcgt cagggcttcc tgaccaccag acaaatcgtt
      661 gctgaattcg gcttgcgcga taacggatgc tgcggccgtt gcgcgaagaa tatccacgaa
      721 cttgtgtcgc tggcgaagag ccagctggag actacctgtc aaaatccggt gcggtcctag
      781 aatcccgatt cggaagcaat caccgcccgc tcgaaccggc attattttcc ctgtgactcg
      841 aatcccctct tgaccgcacc ggcaacgtca gccacccgcc gacccaactc gcgggctcca
      901 gtcagctctt gcgcatcaat gccaggactg tcgccttctg ttgtcgcaga agcgccaaac
      961 gcaccaccac cgctgacgac aatcatctga ttccccagca tcgccgcaag gattgagagc
     1021 atcgtcagct ctttcccgct ggaaatctgg ccgccggttg caaatgcagc ccctaccttg
     1081 ttcttcaatt tgaattccgg gaacacgccg aacttgaatt gccagtcgtc aataaacgtc
     1141 ttcacctcac cggccatgtt ggaccaatag acaggagacc cgagcacaat ggcatcggcg
     1201 gcgaataatt cctcagccgt cactttcccc gcacgcttca ggacaacatg cgtctggggc
     1261 cccgatcgag cgccctctgc aaccgcctcg gccattcgct cggtgtgccc ggaaagagag
     1321 tgatacgcca ccagcacatt gatcgccagc gggctatcag ccgctcggcc aatgatcggc
     1381 caggccagaa gactgctgac gcacagaagc agcactcgcc ccagctgaat catgacgcac
     1441 ctctcactgg agaagaaaac aaccctgaca tcggctgggc caggccatga gcggcctggc
     1501 atgaactagt cgcattttcg aatcgaggat tggggactgg atctccggaa aactggccca
     1561 acagaaccgt agcgcttacc ggcgaacggt ttcttcgtcc atgtgctctt cgacggacac
     1621 ttgggtcaac gttccgcgat agtcgcactt gtggcagcga attctgaatt cctcaatcca
     1681 cttttcctgc acatatgatt ggcggcactt cggacagaca aacacgcctc gcatgtacac
     1741 cacacgtcgc gtttctttca tgaacagcct ccatccaagt caaataatag gccgaggatg
     1801 gcataggcgc cgacgaaatt gcaacacagc gtcgcacact cagcccccgg acacgttcag
     1861 tatcttgacg gcccgcgagg agcccgctag gatgcagccg tgacgctcga cacaacagat
     1921 cgccttgata atacacactg tccggcacta ctgagacgtg cgatgtggtg ccttacaggt
     1981 atcaccatgc tgagtatgac ctgcttcagc ttggccgccg atccgcaggc cgatacgctc
     2041 attacggtca aaacgccaac cggtacactg atccaggcgg aattagccga tacggcattg
     2101 aaacgtgccc agggtttgat gtttcgagag catctcgcgg atgaccgcgg catgctcttt
     2161 atcttcggcg acgcccagcc ctggacattc tggatgaaaa ataccaagat cccactcgat
     2221 atcatctgga tggacgcgaa gaaaacgatc gtccatatcg aacgaaacgt ccccatctgc
     2281 accagacagg acgacggctg tccacagtac cactcggatg aaggtgcgct gtacgtcctg
     2341 gaactcggcg ggggccgtgc agcggcactg cagcttcaac gcggtatgaa actgagcttc
     2401 aaacagccct gaccagcctt caacctgatc actgcgcctt gcgcgcaccc cagaaaaagt
     2461 tctgaatgcg ctcgacgagg gaacgtgtgt gcgtgactgc cgaaagggcc gctgcctcac
     2521 gagacagttg cgctttcgct gccgcagtcg ccgcctgtct acgggccacc acttcaccga
     2581 tgcgctcgat cagtcggcga tcctcctgaa caacctgcag caacgactgc ttcccgacgg
     2641 caatgaccaa caattccgtc gcggccgata ccgtcgccga acgcggctcc ccagttaaca
     2701 gagacatttc gccgaaaaac ttccccgctt caagcgttgt caccgtcgaa ctgagccccc
     2761 cctgttccaa ccgcacatca gcttttccgg aagcgatgac gtagaggacc tctccgggct
     2821 ccccctgacg aacaacccgt tcgccaggta agtaggactc ccatcgcgct tctttcgcca
     2881 acgcttcgag ctgcttggca tccagcgtac ccaaaaagtc gaccgccgcg agttgggcaa
     2941 ccatgcgccc gaccgcctgc ccgttcgtca cgacctccgg ttcgaccgtc tgatgcacca
     3001 cacgctgcgg aaagggaatc tcgattccat tgcgcgcaaa ggcatgccaa atgtacgtcc
     3061 gcatccggct ctcgatcata tcccgctgtg cgaaatcacc gatcgggaat ttgattcgat
     3121 actgaatcgc cgattcgtta aatgccgtga ccagagcggt cgccttcgga tcctgcaata
     3181 cccccggcac cgccacagcc gcctcaagca acgtcgcaca gacctgctca ggcggagtgc
     3241 gataggcggc atcaacatac acgttacaga aatgcgtcgt ggtgggcctg ctgaaattcg
     3301 tcaccccgct ctggatcacc agatcgttag gcagcgagac gagatcctga gcgcgagtca
     3361 gcagcgtcac atgctccatg ccgatgtggg tcaccatccc ttcacgatca accaccgtaa
     3421 tccactcccc caccttcaca atctttccca gctcaattcc cgaaaagaag cgcgtcagcg
     3481 tatctttcaa cgcgacaccg accatcgccg ccgcaacggt cggcaacgcc accagggcca
     3541 cgaggttgat ccccatgacc agccagagga tcaacactcc ggcagccacc acttccgcgc
     3601 cgatgagcag ctgccggaca atatcgggga tatagcgctg ccctcgttcg atcagccatt
     3661 cgccgatgat cagggcatca agaatcgtaa agagaaaaaa agaggcgccc agccaggccg
     3721 caacatcgag tcccgaccgg agaaagtgcg gactgtgact cacgagttcg ctgcggccga
     3781 ccagcgcggc ggtgacgagc accagtgcca caccgccggc atagaacggg agcggcggtg
     3841 ccgacctgcc gcgtcgagcc aacagatagg tcaggcccat tagcaccgcc gccatgccgg
     3901 caagcatcgc cagcaccgtg ccaaccaaca gccccaggct catgcggatc tcacctgatg
     3961 cggggaggag aacacacgaa cacctctcgg gctaggaacg ctcttgccag gagcgaacac
     4021 gtcgcacggt gatgatgcca tcgacccttt caatcgcctt gagcacccga gccagatgtg
     4081 cggtgtccag gacctcgatg atgaaatcca gcatcgcctt ccgatcctcg cgggtcgtaa
     4141 tttcagcccg actgatattg gcctgacatt cggagatggc cgtcgagaca tgcgccagca
     4201 ctccggtctt gtctacggcg atgaccgaga ccttgaccga gtgtgtactg ggcgtggaat
     4261 ggtcccactc gacttcgacc agtcggttct tgtcatagtc caacgcttcc aaattgggac
     4321 aatccacggt atgaatcgtg agtccacgtc ccctggtgat ataccccaag atgcggtcgc
     4381 ccggcacagg attgcaacag cgggagagtt gcatcaacag gtcccgcgcg cctttgacct
     4441 gcacacccgt gtcatcggtc cgtccggtga ccggtttcgg cggcggcgga acctcagggg
     4501 cgaccgtctg tccttccgtc gcgacgggcg gattgatttt gctcatcacc tgcgaggtcg
     4561 gcacatggcc gaagccgacg gctgcggcca gttcctccgg cgtttcatac ccgacctgtt
     4621 tggccacggc caggagctgt tccgaccgca tcatctgggc gggcgccagg ccatgccggc
     4681 ggaactcggc ttccagcaac cgcttgccga tctcgatgct gcgggattgc tcttcggcct
     4741 tgatccaatg tttgatcttg gttttcgcgc gcgacgtgcg gacgaatttc aaccaatccc
     4801 ggtgaggcgt ttgattagcg gcggtcagga tctccaccat gtctccgctt tccatcatgt
     4861 gtttcagcgg aacgatcttg ccgttgacct tcgcccccac acagtggtcg ccgatttcgg
     4921 tgtggacgga ataggcaaaa tccaccggag tggagccttt gggcaattcc ttcaccatgc
     4981 cctggggggt aaagacgtag acgacatcgt gaaaaagatc cagcttcacc gaatccatga
     5041 actgacgatt gtccggcaaa tcctgattcc attccacaaa ctggcgtagc caactgaaaa
     5101 cctttccgtc tttctcgtcg atccgcccgt gctctttgta tttccagtgt gcggcgatgc
     5161 cctgttcaga aacgcgatgc atctcctccg tccgaatctg aaactcgaca tgttcaccct
     5221 ggggaccgac gacggtggtg tgcaacgact gatacatgtt ggatttcgga atcgcgatgt
     5281 agtccttgaa gcggcccggc aacgggcgcc acagtgaatg gatgacaccg aggagcgcat
     5341 aacagttcat cttgatgtcg gtaatgatgc gcagcgccgc gagatcgtac acctcttcga
     5401 atgagatcga ttgcttctcc atcttctggt agatgccgta caggtgtttg gggcggccgg
     5461 agacttcacc ctgcaaaccg gcctcggcca tggccttctt caccaggtcg atgacttcaa
     5521 tgatgtactg ctgccggtct tcgtcccgct tcgccacccg aacgcgcaac gactcgtaga
     5581 catccggctt gagatgtttc agacagaggt cttccagctc gttcttaatc caaccgatcc
     5641 ccagccgatt ggccaacgga gcataaattt ccagcgtctc ctgggcgatt tgttgccgct
     5701 tcccctcgct cagatattcc agggtccgca tgttgtggag ccgatccgcc agcttgatga
     5761 ggacgacgcg gatatcgtcc gccatcgaca agaccatctt gcggaaattt tccgcctgct
     5821 tttcctcgta gttccgaaag gtaatcttgc cgatcttggt cacgccgtcg accagatgca
     5881 ccacgtcctt cccgaatcgc tgttccaact caagcggcgt agccagcgta tcttccagcg
     5941 tgtcgtgcag caggcctgcg acgatggccg tgacatccgt ctttaaatgg gtcagcaggc
     6001 ccgcgaccgc cagaggatgc cgaaaatagg gctcaccgga tcggcgcatc tgtccttcgt
     6061 gcgccttggc cgaaaaatca taagcccggc gcacaagatc gaggtccgcc tcgacgttgt
     6121 agctcttcac ccgatcgatc agctgactca gctctgtcac cgtctcgtgc ggcatagtta
     6181 cgacacctca ccgctcatgc gaagggcctt gatacgcaac tgcacccgat cgtagccgtt
     6241 ccaatgattg cgctcggggc tgaaggccaa gtccaccggt cgaccggccc tgagccccag
     6301 ctcttcaaac gagcccatgc gaaagccgat gctatcgaag ataaatgacc gcccttgccg
     6361 aacacgcaac ttcagatgct tctcgccgac gactcgcgcg tccaccacat ccaggccgcg
     6421 aacagccaag gtgggttcgg ggttgccggc cccgaaggga tgcagggatt ccagttcctg
     6481 gatcaggtcg aaattgacat ccgtcagctt cacctcggca tcgacgtgca gggtcggcac
     6541 cgtgcgagca ctcccggccc actgagccac cacctcacag aaccgccggc ggaattcctc
     6601 cgaccgcgat tccttgagcg tcagtccagc ggcactcggg tgaccgccat aggcctccag
     6661 cagatcctta cactcggaca atccctgata cagatcgaac ccctccaccg tcctggccga
     6721 gcccttccct atgccctgcc catcgatcgc catcacgatg ctcggacgat ggaaccggtc
     6781 caccaagcga gcggcaacaa tccccacaac acccagatgc cagtcacgcg agcctaagac
     6841 gatcgccgcg gccggctcat cctgctgcag ataggtcgcc gcctcgccgg taatgccttc
     6901 ctcgatctgt tgccgctgcc ggttcaactg ctccaactgt tcggcaatct gcatggcctc
     6961 ctgatcggac tccgtcgtca acagccgtac acagagcatg gcatgatcca gccgtcctgc
     7021 ggcattcaag cgggggccga gacggaaacc gatcgtctcg ctcgtacagg ccttcgcgat
     7081 accggctacc tgcttaagcg cgcgaattcc agcgcgggct ccgcgcgtga tgtgggtcag
     7141 gccttcccgc accagaatcc gattctcgtc ctgaagcggc acgatgtcgg ccaccgttgc
     7201 caacgcgacc agatccaagc aggagtcagg ctccacgtcg ccggatccat accgttgctg
     7261 gtaagccgac accaccttat acgccaaccc cgccgagcag agccctttaa acggatagat
     7321 cgcatcgcgc cggtgcggat tgagcacggc aagggcggca ggcatcgtgg catccgtttg
     7381 atgatgatcc gtgacgatca catccatgcc gagttccctg gcaaccgcaa tctcatgatg
     7441 cgaggtcgtc ccgcagtcgg aggtgacaag cagcaaaatc ccctcttccg ctaagcgacg
     7501 aaccgccccc tcgttcaacc cgtagccttc gcgaacccta tgcggaatgt agccacaggc
     7561 attcccgccg agaccttgat agaacgacag atagagactc gtcgccgaga ccccatcgac
     7621 atcgtaatcg ccgtaaaaac agacccgctc ctggcgagcg agggcctgat gtaaccgctc
     7681 gacagccggt tccatatcag ggagcaaaaa tggatcgtgc agcccgccat gcacgccgga
     7741 catccaacgg gtcgcttcat cgggagtcgt gacaccgcgc gcaagcagca ccgacgccgt
     7801 caccggcgag atcgagagct gccgcgacaa attattctgc aaggcgatat cagacggtcg
     7861 aaacacccac agtttttcct gcatgaatcc tctgtctttg cctgaaatat tcagaagtta
     7921 ctgagaagta aggggaatgg tatcgactca ccattttttt gtcaaacggg ttctcgcgcc
     7981 ggataggacg agacgcagcg tcgggtcacg atcagccctc tgatgaacgg acgaagcgtc
     8041 gtggcacaaa aaggagggaa ggaccgggag cggataacaa aaagggaggg gactactccc
     8101 ctcccttttg cacatagttc ttcacgaatt cttcggcatt tttttcgagc acgtcgtcga
     8161 tttcatcgac caacttgtcg atgtcctctt tcattttttt cccggactcg acgacttttg
     8221 gattcggctt gacctcttcc ttctgatgag agtctttcct ggattccggt ctcctctcct
     8281 gtttctccat cggagcacct ccagtcaatc agaacctgtt cgaaccaccc actccttgtc
     8341 accttactct agggtcgtcg aacgcgtcaa gccaaggaac acattgcgta cggagtcgct
     8401 cgcataacgc ccggaacgcc tcacgagcga ccacccccac ctcacacccg cagctatttc
     8461 acaatgagcg agacgatcag cagcgccacg atgttgatga ctttgatcat cggattgatc
     8521 gccggcccgg ccgtatcctt gtacgggtcg ccgaccgtgt ccccagtcac cgccgcctta
     8581 tgcgtatcgg tccccttcag gccctgctct tcaatgtact tcttggcatt gtcccaggcg
     8641 ccgccgccgc tcgtcatgga gatggccaca aagagacccg tcacgatgct ccccaccagc
     8701 actccgccaa gcgcctgtgg tccaaggatg acgcccacca gaagcggggc cacgacggga
     8761 atcaaccccg gaatcatcat cttctgaatc gccgcctgcg tcacgatatc cacgcaggtg
     8821 ccgtactccg gctttccggt accttccata atgcctttga tcgtccggaa ctgacgccga
     8881 acctcttcca caaccagccc ggccgcctgg ccgaccgcct tcatacacaa cgcgccgaaa
     8941 atgaacggca acattccccc gagaaagagc ccgaccagta ccaagggatt ggagagatcg
     9001 aaggtgctgg cttggctacc cttggccacc tcacgggcga actccgcaaa caagaccacc
     9061 gccgcgagtg cggcagatcc gatcgcatag cccttggtga ccgcctttgt cgtattccca
     9121 accgcgtcga gcggatccgt gatgtcgcgc acttctttcc ccagatgggc catttcagca
     9181 atcccaccgg cattgtccgt aatcgggccg aacgcatcga tcgccaccac gatccccgcc
     9241 atcgagagca tcgacaccgc agccacagcg acgccataga gcccacccgc cgccgcgccg
     9301 ccgcaaatcc aatagctgcc gagaatcgcc gcaccaatga cgatcaccgg ggcggatgtt
     9361 gactgcatgc ccaccgccag accggcaatg atgttggtcg catgacccgt ttcgctggcc
     9421 ttggcgatat ccttcaccgg cgcatagtcc ttggacgtgt aataatcggt gatgaacacc
     9481 aatgcgagcg tgaccgccag ccccatcaac gccgccaggt aataactgat accgctcacc
     9541 ccgccgacgc cctccataat cttcgaggtg atcgggtaga acgccacggc cgcaatcccg
     9601 ccggccacaa acaacccctt gtacagggcc ggcatgatct cgccgcccgg gctcaccttc
     9661 acaaacaaaa tgccgatgat ggtcgcgaaa atcgtcacac cgcccaacgc gagcggatac
     9721 agaatcggcg cgctgacgcc cttgaacatt gtgaacgcga gaaccatcgc ggccacggtc
     9781 gtcaccgcgt aggtttcgaa aaggtccgcc gccatgcctg cgcaatcgcc gacgttgtcg
     9841 cccacgttgt cggcgatgac cgccggattg cgaggatcat cttcaggaat acctgcttcc
     9901 actttgccaa ccaggtccgc gcccacgtcg gccgctttgg tgtagatgcc accgccgacg
     9961 cgcgcaaaca ccgagatcaa actgccgcca aagccaaggc tgagcaaggc atgaatcgcc
    10021 ttttcctgcc cggccatctg cgaagccagt gtgtagaaac tggtgatcgc taagaggccg
    10081 agcccgatca gcaacagccc cgtcaccgcg ccgccgcgga acgcgacggt caacgccgca
    10141 ttcatgccgt tgtgtgctgc ttgcgccgtc cgcacgttcg cccggaccgc gatgatcata
    10201 cccacataac cagcgagtgc cgaggcccca gcgccgatta agaatccgat ggctgtcagc
    10261 attccgaact tatccgatac ggcgcccgcc ccccagagca ctacgaacag caccgccgcc
    10321 accataccca ccgtcttgta ctggcgattc atataggcgc cggcaccctc ctgaatcgct
    10381 ttcgcaattt cctgcatctt cgcattaccc gcgttgagct tgaacaccca catggcaaga
    10441 tagaggccat aggcaatacc ggcaatcgcc gcaatcagag caaaggtcac aattgctgag
    10501 tcgctcacag tgttctcctc ctgttaacag aatggtgaaa ctgagaatcc agctcaagtg
    10561 tctacgagaa taccccgtcg cgaacatgaa ccagtcacgt acaacggccg acgaatgtca
    10621 cagaatctcc gtggactaaa cccatcgcga gctgagaatg cagcaggaag aaatactgca
    10681 tgcccgacaa cagacacggt gcgaacgagc gggaaagtgg cgccattcta tagcgaggtc
    10741 ggaatcggat caaggcgaaa gatggccggc gtgaagggtt ggaggcacga cacctcgccc
    10801 gaagcctcgc agctcaccgt ttgcggaagg acagcgactt tgttatagtg cgcggattga
    10861 ggagtactcg atgggactgg cgccggaata tatcctgatc gatcagcaaa agttcagtaa
    10921 aaccaagctg gcgctggaca atcacgtgta taagaattgc gagatcgacg actgcgatgt
    10981 gtactacagc ggcggacagt acgaattgat cgatacgcac atcaccaact cacgactcat
    11041 cctcaaccac ccggccaagg gcatctacag cgccattcag atcttcaaga tgaagtcccc
    11101 cggttccagc atcatctccg agtaatccgc acttcgtgac gcgtcgttcg gctctcgtgt
    11161 gaccgtaaga cacgtggtct tggacgagat acgcttcacg aataacgggc aacgctacct
    11221 ggcgaccgga atgttctcgc tgaacttcgc aatggggatg gattgaaaca caggatcctg
    11281 cgcccccttg gaggattccg cctcaatgcg ctgcaaaaag gcagatggcc cggtcacagg
    11341 agcgtaactg agcatggcct cgacgtcgaa ggtcttggtg tccttcggtg tcgtgaaggt
    11401 aaacgtttcc acgcgctttt cttccggctt gagcaccgtt tcttcaagca ccttgatggc
    11461 ctcaaaatcg aacaccgtct tctggccttt ggcgtcggcg taggtccggc cgtagacgcg
    11521 tttgtcgttg aacacggtct tcaggttctt gcccttgata tccaattcca agacgacact
    11581 cgcccaaccg gggtgggtgg tcggcaggtt gtgcgggacc aggctttgca ccgctaccgt
    11641 cacggtagtt ttctcccctt ctactttggt cgagacttcc agtttggccg cttcctgcct
    11701 gagctgaccg atgcggcccg ggaacgtatg attcgccacc ttccgcttgg cttctccatt
    11761 ggcggattcc cctacctgct caggcatatg acaggtctgg cattccttgc ccgacttcac
    11821 ggctttgctc tggtcccaat tccccaacaa gtcgttactc ttgccgaggt ccgccgcagc
    11881 gggcaccacc tggtggcaat tcaagcagag gtcagacttg tggaagagcc ccaattccat
    11941 ggattgatgc gcaaggttct gcgcgaaatc tttgtatggg ccatacatgg tcttgctgcc
    12001 gagatcgtac ttcggctcag gcggttgttt cgtccgatcg acctgcttga tgaggtgaca
    12061 ttgcacgcag gccacaccat ccaatgacgg ctcaccggat tgggcctgag tcacgaacaa
    12121 ttgggcctga tcggcaaact cgcgcacatg cggggcatga cagcgaaagc acagggcttt
    12181 gtctttcccg gcagaggagg tcaggtaggc atcgagcgcc gcccggaagg tcggcgtatg
    12241 tatcgaacgc gaaagcggcg acgtctccca ctcttcaaac acacgctcat gacatcgctt
    12301 acacttgctt gagctgggaa aagccttttc gatcgtggcc tgggccttgg catcttgggc
    12361 taatccctgc tgcacgccgt agctggtgat ggccaccgcc agcaacacca ggccgatcac
    12421 gaatccctgc agcaatcctc gtcgattctt caccgtcggc gtttccttcg cacagtactc
    12481 aatcctcgct gcctgtggtg cacggcccgc tgcaggggag cttagagtga tttggcccga
    12541 taggtcagca tacgaggctg aatgtattcc tcaatgacct ttttagcggt cgcccgctct
    12601 tgatcgttcg ccagagtcgc cagatacttc gccttgagct ccgcttccgg ttccgggatc
    12661 aatgcataac tgagcacgac ctcaatcgtc gccggcgcgg cgccatcctt cagcgtttga
    12721 gagaacggga cacggcgcgt attgccgccc ttgatgaacg ggtccggaat cgggccgcgc
    12781 agaatcttgt cgtacggaag accgaatcgt ttcgtgtctt cacccaacac attcccttgc
    12841 gcatccttga tcgtgatcgc aaggaagaat tgcttcaaca ccggatcgcc gtcggggaaa
    12901 ctatgcggca gactgccgac tttcaccagc acagtgcctt ccactcccgc tccggatttc
    12961 gtggcgtcta tctcgatacg cggcatccat tccgcctgca ggttgcggtt cttcagcaac
    13021 gtccctggaa tgacaacgcc tcgaaaccaa tggcggccga tcgcgcgggt catggcgccg
    13081 cgtttcgacg tggagcttcc cgtggagggc tccatatggc aatcctggca gatggtccct
    13141 tgcagaatct cgccgggtaa atccttctgc gtgacatcct tcaccttgtc gaaatggcag
    13201 gacgtgcaga aattcgcgcc acgaaacagc ggcaactgtg tcgccggatg taccaagttc
    13261 tcttccggat cggcataggg accgtagagc atctcgcccg gctgcacctt gtaggttgga
    13321 gggggcagcg gcgtcgtttc gacggcgttg atgagatgac aggacgcaca gccgattccc
    13381 tcgacctgcg gcttgcctga caggatttgc gcgctgatct tctcgacatg ctgcgggaag
    13441 accgtcacgg ccggggcatg acacgacaaa caacgttgtc gctcctgcat cgtcggattg
    13501 gtctgcatcc atacccccag caccgtccgg aacaccggcg attcgaacga ggtaccatgc
    13561 aacaaggcgg catcgacccg cccgaacgat ttcaggtccg gagtttgctc gcgcatgccc
    13621 ttccattctt cgtaatgacg atcgtggcac tgcttgcaat cttccgaccg gatgaaaatc
    13681 ttttcgatct ccgccaccca atcagccggt acctggccat cggtcttctg cgcgatcgcc
    13741 cgttcatgaa aggtgatgtt gagcagtgcc atgaacagca gcaccccacc catcgccaac
    13801 ttttgaatcg atcgtccctt gtcgttcacg ctgcgctcat gaccggttcg gatttagtcc
    13861 cgcttgcacc cgacaaaatg aaaattcacg ccccagccga ccggatcgcc cggatctgcc
    13921 ggctcatagg tcccgacggt aaaattgcac tctttcatcg ccgccttgac ggccttcccg
    13981 aaatcgatca ggttgcggac ttccttcttg tcgatctcct tgatcaccga ccgcaccttg
    14041 atccccgcgt agtccgctct ggaattgccg atcacttcaa agaccgccac gccttcaggc
    14101 cgctgtaact tgagttcttt ctgtacctct tcatccactt caccgaccgc gatcccgaac
    14161 tcttccccga gttgcacggc ttcctccgcc gtcatctcgc cgttcatacc ggtacccgca
    14221 tcggcctgga agccccatcc catcacggtg aacaaggccc cacacacaca aagagccctt
    14281 ccgagagaaa gggctctggt gaaaatcgtt cgattcttct tggacattcc ctccaacggg
    14341 ttcgcctcat ccgcacaggt tgaaccggaa ccggcacgtc ctgccgacag tcatatccta
    14401 ccaggaagaa ttacgtcttt gtggccggat ccagcgcgag ttgaaaccag ggggcaatgg
    14461 ctttctgtcc gttacgctct ttgttctcgc cgttccacac ggcaaaagac acggcctgaa
    14521 tacgacccgg aatcagcttc gcttcattct cctgctcttc actcgacagc ggccgacgca
    14581 tcacgactcg ccacgtgccg tctttccagg tcgcttttcc ctgcacgcgc ccctgcttct
    14641 ccttggtggt cagggtgctg aacccgccgc cgatcaaatc ttccaccgag gacgcccgtc
    14701 gaggaatgac ctcaaagcgc cggggttcac caatggccgc cttgtctttt tccgtggtcc
    14761 tggaagcacg ccggtcgatg tcgctctgcc aatcggcttt ccaatgccaa atattgatgt
    14821 aatgatcgag ctgacccata cagaaaaatg ccggcgcatc gccgagcggc aatcccagcg
    14881 caacaccgtc acggaaggtg ccaggcgtca agcgatcgtt tttcgtattg tcctgccatt
    14941 ccaataagaa ggcgatgtcg gttccgttgt gcacggcgcg gactgccaac gcatgggccg
    15001 tcggctccgg ccacaccggc cgggtgatca cctggccgct caatggcaag gtcatgggcg
    15061 aaattttctg ccagagggga tcttcgggcg cggtcgggat gtctcccgag agggcctgtg
    15121 cacggatgat catgccttct gaactgacga tggggatacc gagaccgccc agaatgaggg
    15181 cgagaacgaa caggctggag agaagtgtca gtaaccgagt gcgggatgtg tgaaccgatg
    15241 tcattcgcct catgtcccga cgcaatccgc taaccgcgaa aactcccgca cccgctgtgt
    15301 gcgcgggaag ctgaatccgt tccccaaatt accatagctt ggcgcgacga atcttatttt
    15361 caccccgctc acagatatac aagtacccct gcgtgtcgag cgtcagccca accggagaat
    15421 taaccacggc ctcgacggcg agcagaccat caccatgctt aagcgcgccg gcggcatctt
    15481 tggcgacaaa ccgcgccgcg ccacagccac ccatgttctt atcatcgtac ccgctgtcac
    15541 cgttaccggc caccgtgctg atgaagcccg tgtccgcatc caccttccgg acccgatggt
    15601 tgccggtgtc agagatatag gcatgattgt gttgatcgaa gacaatggct tccggcgcgt
    15661 gcaacatcga cttcgcggcc ggcttcccgt ctccatcata tccataccgg cacacaccag
    15721 cgagggtgga aatcagcccg gtcttatgat cgatcttgcg cactcgatta ctccctttgt
    15781 cgacgacata cacatcaccg gcattgtcga cggcgatacc gacgatatcg tacagccgcg
    15841 cctccatggc cggatgtccg tcatcgaggt acatcgacaa actgagccca tccgaacaga
    15901 ccggcatcag ccacccgttg tcgtcactga agtcgatgcc gatcgcatcg ccggacaaca
    15961 ccaccaggtt gcgcgccacc aatggctgct cccgggcttc gtcttccgcg gtccagcttc
    16021 ccgcgatggt ttccaccagg ccggttcgcg agtcgtaccg acgaatccgg tgcgcctgcg
    16081 tgtcggcgat atacatcaca tcgtccgcgt cgaaggcgat cgccgcgggc caggtcaaat
    16141 tcacttccaa cgccgggcca tcaccattaa atccatgttc accggtgccc acgaccgtgg
    16201 tgataattcc ggtttcacga tcgatttttc gaatccggtt actgccggaa tcacagacat
    16261 agagatcgtt acgagagtca caggcgacgt ccagcggcag atacagtccg gcttctccgc
    16321 aaggcccttc gtcgccgctg tagcaggtct caccgatccc ggcaaaatta tggacggtac
    16381 cctccgcaag attgacgcga cgaacccggt cgctgcctga ttcggcgaaa tacagccagc
    16441 gctcgtcccg atccagcgcg acgtggtgcg gcagcggaat gcctgctttc acagcgcgct
    16501 tgccgtcccc cgtgcttcgc gccttcccgt ttccggcaaa ggtttcaatg tatccgaccg
    16561 ctaattgaac gtccgtttcc ataactgtgt caatccgttc tgtgctgggc ccattgtgtc
    16621 gaaggatgct acgcgcgcgc ggccgggggc gctgcggcga ccggctgctc aacgacaggc
    16681 gtggacattg ccaccggcgg cggggcctga atagcctgag cctggaccgg ttcggcctgt
    16741 tgagcctgcg cttgcgcctg ggcttcagcc tcgatcgcat ccgcctcatg aacggatttc
    16801 ttgaagccct tgatcgcttt gccgatcccc tcgccaagct gcggcaactt ccctgccccg
    16861 aaaataatca gcacaataaa caggatcagg atcaactctg taaaaccaag actgccaaac
    16921 atggtgtgtc tcctttgctc acatgttcag gatgcggctc cctctttggc tttcttcgcg
    16981 aatcgttctt tcaagcgctg gcgagcctgt tcggcctgct cgaacatctt gcacttgtcg
    17041 tagacgctga tcaagttgta gtaggcgagg ggctcgtccg ggctgtgctc caccgcgctc
    17101 tccatgacct tgatcgccaa atctgtcttc cgatgattga gggcgatttc catcagtttg
    17161 aagctcgcct ccagatgctt cggatccaac tccagaacac gaagataaca ctggattgcc
    17221 atgtccatcg tgttgtaatc ggagacctgt ggattgtcga gttccacgta cacaccggcg
    17281 agattgtacc atgccatcgg atcatccggg gtgatctcga cgagccgctc gtagtaattc
    17341 ttcgactcca tgtactttcg tttgtccgcc tgcacgcggc ccagattgaa cagggcaagc
    17401 acatcgtagg catgcacatc gagcgcgcgc ttgaattccg cttcagcctc gtcgatcata
    17461 cccttggtgg catagatggt cccgaggttg gagtagtaca tcgcgagcga ccggttcatc
    17521 tcggtccgga gcccctccgc catctcgatc gattttttca cttcggtgag tgcgtcgtcg
    17581 agccggcctt tgctgaaata cagttccccc agcctgcacc gtgcctgaaa atcatcgggc
    17641 tcggcaccga gcaacttttc aatctccgca atttcttcgt cggggctcaa cggttgatcg
    17701 ccgggatcca ccgccgcact cccctgctct gctccgcttg cctgtgtctg ttcatccata
    17761 acaacgtatc ctaatctggg tgactcacaa gattaaattt gtccaccaac gagagattcc
    17821 tgcgtgccga cgaggggtgg cctggtcacc gccgcctgtt ccgtcaccgc acctttggga
    17881 cgatcaagcg tcatcagcat caccccgagc accaccatca gggtcaaact ggaaaagagc
    17941 aatgcatagc ccgcaataaa catcaacgcc aggccgtatc ccgcaagacg ccccatcgtc
    18001 acgagcctga tcacggtgta cgaccaacag agcagccccg cgacataaaa ggcaaagggc
    18061 aggtaaaagg tataccccat gcccatctga aagatccagc tgatcccctc cccggctttc
    18121 cggatggaat gggccaacac cgggttgtag agccccagga aataatccat aaacagtaag
    18181 ccaaagaaca ccaccgccga gacggcgctg aaccagatcg cgcgccggcg ctgctgtcga
    18241 ttcctggtac ggaacgacac cccgcgccca taggcccaga acaccaggat gctggcaagc
    18301 accatcagcg cttcgccgag acgattggct tcatacacaa atggcggtgc ggcaatcacc
    18361 cccatccacc cataggcggt ggagaagatt tgataataga gccatccgga aatgccgaag
    18421 taatagaccg cgcccatcac tcgcttcgac cactcctgct gctgggagag atactccagc
    18481 atcagcgcgg tgagtgcaat gagggtcacg acattgtagg cgatggagcc cagcatggcc
    18541 ggcgggacaa tcagaaagga caccgtgagg atcaacagga gggccacgct gggaatagtg
    18601 acgacattaa accccgtgat gcccttggcg cgagccctgt tcaccagcag aaccaccagg
    18661 gccagaaata caaaaatcgc gacgacattc agcaaggcga agccgacaga cgtcagcaac
    18721 tggaagatcg ttccgaccca ctcatgctgg gccgcaatct tgctgatatg catgccgagt
    18781 cgggatacca ggcgatagag gacgagttcg aaaaatccga cgaacaacac cagcttgatg
    18841 gtgtattcga acaacagtcc ctgatcttcg acccgcccgg ttgaccgttc aacggcggtg
    18901 gctacagcag acatctccag ccccctgtgc aaaacgttga tgataatcct ctgaccacaa
    18961 ccggtcaacg cggcaggagc ctgccctgat gaccgcacga acgctaagag ccggtcggct
    19021 gtggtacctg caacgcttgc gacttggccc gagcgatata gagcagcccg tcggccatag
    19081 aatagttaaa cggcaattcg cacacgactt cgctgatttt ttcgtaaacc tgttgataga
    19141 gttgctcggc ctgagcaggg gtcatgccct cctgcacttc gtaaaagaag cccaggctca
    19201 ggtcttcggt cgccggccgc atgatccgac ggatgccgta actgcccgga tcactcatga
    19261 tcggcgcctc tttttccagg tcgaacaagc agggctgaca ggagaacccg taggactcgt
    19321 ggagcttctt attccccacc acgaagttca tcgtatcgag ggcttcctct tccttttccg
    19381 tggggaaccc gacgatcacg tagcagtgca cggcaatccc aagatcgacg cagtcatcga
    19441 cgatccggcg cacggactcc tgcttgatcc cttttttcat aaagtccatg actcgctgat
    19501 tgaacgactc caggccgaac acaattttca aacagccggc gtccctcatc tggccaagca
    19561 attcacgcgt gaggttcttc tcgaaacgca actcgcaggt ccacttcaca ttcacttgcc
    19621 gatcgatcat ctgctggcac aaacgcttgg tgggagagag ggcgaaacat tcgtcggtga
    19681 agaaaaagga ctgcgctcca tagcgctctt tcaaccagac caaatcgtcc accgtcttcc
    19741 ccggatcacg ctgccggaaa ttctggtgat ccagcgtgag ggcgcagaac gcgcaatctt
    19801 tgtagtagca gcctcgtgaa aattgtaccg gcaggacagg ctcgggggac aaatacagat
    19861 ccagcggaaa gccatcgtag ttcggcgctg tgagctggtt gatattctca gagtaaaagg
    19921 gctgattgac cgtaatctta ccgttctgac gataaatcag attcgggacc ttactgaaat
    19981 cccgcttgcc atccatctga ttcaccaact ccagcaaggc tgtttcacct tcaaacacga
    20041 cgaaatcatc gacgagatcg aagagccacg gacagcggcg cagattatca accaaccggg
    20101 tgaagatact tcctccgacc gtcacgtgta gctccggagc atgctccttg atcaggcgac
    20161 agagcgtgag gcctggaata atttgcgagg tcgctgtaat cgagacaccg accagatccg
    20221 gacgatcagt gaggagcgaa ggcaacacgt gctcgcggaa gagactaata tagggattct
    20281 gcccttcgtc acgaatggcc ttgatcagat ctttcgagga gtagatcgaa tagtcgccga
    20341 attggttatc gaccactgtc atccgcgtcg gaaagtacag cgaggagaga acctcaagcc
    20401 atttatcgat cagaaagaga ctattgcgat aggcctcaat atcgtagaac ccttcacctc
    20461 gcagcgtctc ttttgccagt tcgattcgct caaacaaata gggaaaccgg tcaagcgact
    20521 cgatcacgcg agcaagttgc tctgcgctcc cgggccccct ctcaccgatt cgctctcgct
    20581 ccagaccttg ctgtttgtcc accaactgct ggtagagccc atgcgcaaag gattgcgtca
    20641 aaaccttgtc caacaactca atgccgaggt cacgttgaga gacgtttttg actccggctt
    20701 gggtgaggaa tccggtgagt gatggaaggc tgaggtacgg ttgggatggg tgccacgtag
    20761 gaggaaacag gagtgaaact ttcataggat tatctttata ttaagcagga aaatcagtat
    20821 tttcagaaga gttgcaccgt atcggtatat acactccgct ccagtccgaa tgcaaccctc
    20881 cgccctatgc acgccaagaa atttcaggcc ttcccgaggt tgaggcactc tctgacaaaa
    20941 cgcacgaagg ccccgaatca ctttgccatt tctggcaacg actcggggcc ttcctgttaa
    21001 taatcacaca cgattcctac ccggcacagg agtaggattg gccacttcag gccactaggc
    21061 tcacggcatc ttcagcgtga accaggtgga gagcgctttc ataccgtttc gttcaatgtt
    21121 ggaaccatcc cagattgcga aggcgattgg caccgaagcg cccgccttga actgggtgtc
    21181 gttggcatcg ctggtctcca gcgaacgctt cacgactaca cgccaggtcg ggccggtgta
    21241 tccaccgccc ttgagggacc cggacggctc ccacacacca ttgccgatca catcctgatg
    21301 ggcctgggtg gtcaaggtgc tgaacccgtt ggcattcaga tcctcgacgg agctgacgcg
    21361 cagggtcggg tcggacatga tgttaccgga ccaaatgccg gaattgaatg ggccaaggct
    21421 ccggccaata cgatccggat aggtcactcc accagccggc tcttcgaaat agtaatccca
    21481 gaagatgcca gggtattggt catccacgtc ccacatccca gcgctatcct tgccgagatc
    21541 cttctgccac tccgcgttcc aacgccagat gttgaccgtc ccgcctgact ggcccatgca
    21601 ctggaatggc ggggctcccg cggtgttgac cgggaacatg acggcggcct ggtcccggaa
    21661 atcctgcgga ccgattgccg tgtcgttctt ggtctggtcg ctccaatcaa ggcgcaaccc
    21721 caaatccttc ccattgctga ccgccttcac aaacaccgac ttcaccgaaa tattcgggtg
    21781 catcggggtc gtgatggtct ggccgctcaa tgggatgatg acacctggca ctccttccca
    21841 gatggggttt gcaccatcca tcgggattac ccccttgatc gccttcacgg gaatggtcac
    21901 cggttggctg accgcgagag gcacctgccc aatcgtcaac ataatgccga cgatcagcgc
    21961 agagaacaga atgccaaaca ccaaacgctt attgcgtgtc tgcaccagtc tcattgcgta
    22021 tgccctcctc tgaaagatta ataagaaaaa gaactgggaa cgtataacgt ctgccgaaaa
    22081 ccgaatactg cactgcccga aaaccgagaa ctcctacgca gtcccgactg ggccttcgct
    22141 cttctcgccg cccttctctt tcgtctgttg gtccatgaag gactcagcgc cgacctcact
    22201 cagcaacatg ctgagctcat tccccttgtc ctgcgccccg cactcgtagg tttgaccttc
    22261 cggagcattg aaggtagctg gtctatagtc cgcctcagag tacggttgca cggtcactcc
    22321 gaggaaggcg acctcaaaat ccatccactc cgacatacag tcggcaataa gcttgaataa
    22381 gcccgtatcc gacttcttcg ccaacattct gcagaacaac ggcacccatc ggccgatatg
    22441 attcttgata aatttctttt gcgcatcgac cacgatctcg gtcttgtcga taccatcgtg
    22501 gcagcgcgaa taggattctt tgtaggtcag aaaatgcatg aactcgagct cgacactcag
    22561 gtgatcgaga cgctcgtgca cgtcctttga gagctcgacg ccgaaggctt tgtagaagcc
    22621 tgcaatgtcg cccatcacat gggattgcgc aaaaacgtga tcgttaccga acagtgtttc
    22681 gtagggcgga caatccagcg tgatcacatt ggtgaagacc cgccgatgct ctgtctgaag
    22741 atcgccgatc tgccaattga cacactcagc cgaaaccagt ttctcgatct ggtcgaactg
    22801 ttttttcatc agggcgattt tctggctggc ccgatcaccg ccgatgccat cgagcgcgag
    22861 acgcaatccg tccaatgcgg ctcgtccatc ttcaacaaat tcaccacact ggagataatc
    22921 caggaactcc tcgtcctcag gatacaacaa actccatgaa aacaagagat atattttgct
    22981 gcggttgagc gcgcgttcga cagcgggcga atcttttata gcgaccgcct gcgggggagc
    23041 gattgcggtg gaggacgggg aggtgggctg cacgacctgt ttcgatgtca tacgtatctt
    23101 gctcctcacc tcaacatgtt gcgcattttt ttcgcacact acgcaacatc tgagtcctgc
    23161 ggcacaggag gcgtatgata ggtaaaggag ggagggatgt caagcatgaa ctctctctcc
    23221 tttgcccttg ccgcaagtgc cgatgtttca ctactctgcc tgatcatttc gcattccgga
    23281 acgagtaaac acacacttta ttcagtagcc cagcgcaccg gcagacaggt ttcttgccgg
    23341 tgtcaccttg atcgcaatac gatcattgac cgtcttgcaa atctgctcac acataccaca
    23401 tccgacgcac cgctccggag ccaccaccag atgaagcgcg tgaaaatcca tcgagagggc
    23461 ctcaacaggg cattttgaca cacaggcatg acaaccctgg cccgccgtac acacacggtg
    23521 cgacaccgtt gccacaccca tccgcacgtc aaaacaatcg gcaaccggca ggagcgcctc
    23581 ggtcgcacag gcggcaatac agggaaaatc gtcgcacaat tcgcatgcga cctgattcga
    23641 aaaaatgacc ggcgtaccgt tcgccacatc gctcacgatc gctcccggcg ggcatacttt
    23701 aatgcaatcg ctacagcgcg tgcaccgttc aagaaacatg gcctcattga cagcacccgg
    23761 cggacgcagc cagtctgttc gcagcggcgc ggccggttgc tcgcggggcg cgtccttatg
    23821 agcggcaaat tcccgcgccg ccttcgccac cgacacaacg gagtctttca ggaaatcacg
    23881 acgcccgtat ttaggatcag cggccacgat gcaccccgct tacatcacac cgtcggcccg
    23941 caacttgtac acttctacac aacgatcaca ggccccgtac tcgacatccc agcctggatg
    24001 gttctcgcgg atgaagtcca agatatattt ttccagcttg gtctccatat cttccaccca
    24061 ggtgtacgtc gggaaccggc agagcggaca aggaaacccc ggcatgagca tcaccttatt
    24121 ggtggtctcc ggaatttctc cgccctcgac atccaccgcg cgatccagga tgcgcagggt
    24181 atcagtggcc atttccacca attcggaatg ggtaaaatag ctcgtctgcc acaacccctc
    24241 aaatacggat ttcaactgcg ccggcggaat ctttcgatac cacgaccgga actccttgaa
    24301 tcggtcttcc ttctggagca tcggctcctt cccggaggcg atcaagcggc tatcgacgct
    24361 gatattccaa agaatacgat agcggtgcag gatgagcgtt tcttcgccgg gattctgtcc
    24421 gacccgcgta tcgggatcgt acccgaacgc cgggtcgagc atgtcatgga tgtgcaggag
    24481 ttcgtgacga cagtaccgcg tcaacgccgg atcgtagaac cgccgcggaa tcagcttgat
    24541 accgactccc ttcagtccct tctcctcgaa ttgcttcgcc aggtcgtgct cgacggagcc
    24601 ccatttgcgc aggacgtcca ccccttcctg atcctccttg agcaccccct tgaccaggac
    24661 gattccgact cgctgcttca gctcaggaaa ttcattgaac gcgtcgcgga tgatatcgga
    24721 gaacccccag ataccgaata agtactgata cagcttcttg aactcggcct cgcgatcgtc
    24781 cagcgtgaac ttttcataga tcgggtcggc cagttcatgg aactctttat agtaggtcgg
    24841 atccccttcc cgctccgtct tttcaataaa ggagtcgatg acttcctgca gtaaggccgg
    24901 ctgaaaacgg atctccatcg acggcttgga aacttctact ccggttgcca taagacctca
    24961 catgttctgg ttcggtgaag ggatgaaacc cgcacgggta ctgtgcttga cttgctccca
    25021 gtcgttctct tacgatcatc gaaacgaccg ccgattatac aaacccctta gcgatttttg
    25081 caaaccgttg agggtcccgg ccggggctcg atggcccccg ctcagcacag cgacgaggca
    25141 cagactttta tgagcaatca aacgaccgcc cccctgaccg cgccttcaac cggacaacca
    25201 agcagccaga gcggaaccgc acagcccccc cctcccgtcg accatttaga atattggaaa
    25261 gcagggcttc gagaactcaa gacgtttcgg ggacactccc acggcgtctg gtccgtcgcc
    25321 tacgctcccg atggccagac gattgtcagt ggcggcgtcg atcgatacgt ccgcgtctgg
    25381 gacattgaaa ccgggcgact cctgcgttcg ctgaggggcc acaccgccga cattcgcgcc
    25441 ctggtgttca cgccggacgg ccgcacgctg gcctcaggca gcgaagatcg aaccatccgc
    25501 ctgtggaacc cgaaaaccgg cgagccgacc aaactcttat ttacccgcta cgatcacaat
    25561 gtctgcagcc tgtcgttgtc tcctgacggc ctcatgctgg cccgcggcag tcacaataaa
    25621 gacatcaaga tctgggaagt cacgaccggc accgacctca tgaccctgct gggaaaagac
    25681 caatacgatc accactggtc ggtctgtgtg gcgttctcgc cggacggcgt gcacctcgcg
    25741 agcgggtccg acatcggcaa aatcagaatc tgggaagtgc tgccgagcgg agaggaaaaa
    25801 atcctccata acggccattg ggaagaaact gctgaagact cgaccgagac ccgcggcttt
    25861 ttcatcgaag acgacggagg atttcagaag ccgatggagt actggatcgg cgcgatggtc
    25921 tttacgccgg atgccaaaat gctgatcacc ggcagccgtg acaacacaat ccgctttttc
    25981 gagatgccga cgatgaacga gctgcgcgtc gtgcgcgggc ataatggatg ggttcggtcg
    26041 ttagtggtct cgccagacgg aaaagtgctc atcagtgcag gggatgacaa cacgatccgc
    26101 ttctgggaca tcgccacggg ccgaaatttc cgaaccgaca agacccatgg cggagcggta
    26161 cgcggtatcg ccctctcgcc ggacggacta cgcctggcca gcgcctcctg ggatcgtacg
    26221 gtgaagctct gggaaggtgg cgtggagccc gccgagtaac ctactcatcc ttctcctccg
    26281 cctcaccctt ggcggaggag agaccgtacc gcttacattt gtcccacaag gcttttcggg
    26341 atactcccag gatcgtggcg gcattcgtcc ggctgccgtc tacctgtttc aaaactgcgg
    26401 caatatagtc ccgctcgaat gcctcccgtg ccgtcgcgag cgtggcagga gccgtggact
    26461 ccggcttctc acccatcgcc gtcagtcctt ccgaacaaaa accgcaacct gcttgaggtg
    26521 acccgccgag atagggacag gtctggaagc cacacaggtc ccacggctgt aacgattccc
    26581 cgtcccgccc caaggcggcg gctcggtcca ccatctgctc cagttcaccg acattgccgg
    26641 gaaacgaata gtgcaggagc agatcgcgac tcggctgcgc aaacccatcg agagatttgc
    26701 ccagagccgc cgacctcgcc tccagcatat gctcggcgat caccagcaca tcttcccgcc
    26761 gctcgcgcaa tggcggcaca acgatctgca cggccgtgag ctgatcggag agttctttgt
    26821 ggaaccgtcc ctggtaagcc atccccttca gatcatcctt ggacgcacac acgatccgga
    26881 cgtccacctc gatgtgttcg cgcccgccga cacgctcgaa tcgccgattc tgaagcacgc
    26941 gccacaccct tgcctgcaca tttggagaga ccgcatcgat atcgtcaata agcagggttc
    27001 ccttgtgcgc caattcgaac cgtccacgtc gttggcgcag cgccccggga aacgcgccct
    27061 tttcatgacc gaataactct gcctccagga gcgcctcggg aaggtccgcg caacagacct
    27121 tgatcagggg ctgatcgcgc cggggactat tcaaatgaat ggcatgggct accaactcct
    27181 tgcccgtccc gcgctccccg acaatcgaca ccggcgaatc ggttgcggcc actaacttga
    27241 tcttctccag cagggcccgc atctgatgat tgcacccgag gatcccgccg aaactgaacc
    27301 ggtcttccag cgcctctttc aattcaagat tttcccgtcg caatccaaga attttcccga
    27361 cacgctcgac aatcagcagc agctcgtcca tttgaaacgg cttggtgatg tagtcgtatg
    27421 cacccagctt gatcgcaccg accgccgtat ccaccgaccc atgggccgta atcaggatca
    27481 cttccgtcgt cgggctccgc tccttgcagg ccttcaaaat cgtcagtccg tcggcgccgg
    27541 gaaggcgcag gtcggtaatc acgatatcga actgccgcgt gcccaggatc gtcagccctt
    27601 ctgttccagt ggcggccgcc atgacctcac agccgatgcc ctccaaagca tccagcatgg
    27661 acaaccgcat caacggctca tcatcaacaa tcagtacttt gaggcccttc acgaaaacct
    27721 cccgatggcg tgccgttccg tcgcaagcgg aatagagacg gtaaaggtgg tccctttgcc
    27781 gacttcgctc tcgacaagaa tccttcctcc atgccgctcc acaataccca ggctgacgga
    27841 caggccaagc ccggtgccct ctccttcatt cttcgtggtg aagaagggat cgaaaatccg
    27901 cggcaacacc gaggaggaaa tgccgcagcc tgaatcctga atccgaacca ggcagtgggc
    27961 ctcgtccatc gacgtacgga tggtcaggac tccaccggtc cgcatcgctt gaatggcgtt
    28021 caacacaaga ttcatcaaca cctgctccat catatgccga tcgatcatca actccgggat
    28081 gtcgggcgac aacgccgtct ccagctgcac acggtggggg acaaacagat ggtccgtcaa
    28141 cagcaatacc cgattgacaa cttgattgat atcagccagt gcgagttccg gattgtgttg
    28201 ctgcgagaaa tcgagcagtt gccggacgat tcgttgcacc cgccgcagtc catcctccat
    28261 cgacgccagg tactcctcct gcctggacgg cgacagggtt cccttccgca tattgtacag
    28321 acaattaaga atgcccccga gtgggttgtt gatctcgtgc gcaacacccg cagccagttt
    28381 tcctaccgac gccagcttct cggagtttct gatttgttgc tccagcttct tcgtgtccgt
    28441 catatcgcgg gcaattccca gcacgcctag gatctccccc tccacgccat gaagcgggga
    28501 gacgctgacc atcacggaac gcggctcgcc ggccttcgtc aacacttcga cctcatagac
    28561 ctgttttaca ccgatatcca gcgtcgattt caaccggcgc ccccgatgcc gtttcgacag
    28621 taaccccaga tacggccgcc ccaacagatc ttccttggca taaccccaag ccagcacctt
    28681 gctgttcaca tacgtgaaac actgctcgct atcgagcgtg taaatgacgt cattggcgtt
    28741 ctccaggagg ttctcaagat actgcttggt ttcttcgatc tcgcgggttc gctcgctgac
    28801 cttcgcttcc agctcctgct ggtaggtatg catctgccgc tcaagacgtt tccggtcggt
    28861 gatgtcgcgc aactggacca tgacccaggt atggtcgctg ccgcgcacca gaatcaggtc
    28921 catctccacc ggcgtttccc tgccggtgac atcgcgcacg gtgatttcct gggtcggcac
    28981 ctggcgctcg cccgcgcgga tcttttccaa caacacccgc atctcgtcat ggtgaaccgg
    29041 ttcgaccacg ttgagaatac tgcttccgac gacctgttgc tccgagtagc ccagcgcctg
    29101 ctcttcccgt ttgttgacgg ccacaatcac gccgtcctcc cccaccataa acaccgaatc
    29161 cgccaccagg tcgaacaatg ccttataccg ttcttgcgag gccaccagtt gccgggtgcg
    29221 ctcgttgacc gcctgttcca gtctggtcgt gtactgatgg atttgcgcct ccaacagccg
    29281 ccgctccgtg acatcgcgga caaacgcgcg cgagtgaacc agaccgccac cgctttcgaa
    29341 tagggccgtc gcatgaattt ccacgtcgat cgggtggcca tccccggcca caaacacggt
    29401 ctctatcgta ctgcgcccct gcgagaccaa ccgctccaga taggccaaca cctccgcctc
    29461 acggcctttg ggaacgagat cccagaggcg catctggagc atctcctcca gggaataacc
    29521 gagcttgtcc agaccggtct tattcacgtg cacaaactgg ccggctttgt tcaactgata
    29581 gatcatctcc ggggaatgtt cgatgagatc gcgataccgc gcctccagcc ggcggacatc
    29641 ttccattcgc tgctcgatct gttcgaacga ggtacgcaga tttgccgcca tcttattgaa
    29701 ggcgtccgcc agttgctcga tttcatcgcc ggtcttgaga tccagctgct gatccaaccg
    29761 tccgcttcca atccgctgca ctccttcgtg caacagtccg atcggacggg cgatgcgtcg
    29821 cgcgacggtc agacccgtga gtaacaggac gccgaacacc cccagcccat acaccgtcac
    29881 ttgcacgacg agccgcgaga gcggcgcata ggactcggcc gggtcctgcc gtacaaatgt
    29941 cacccagcgc ttgccaccca ggctatccgg cgtcaattca gtccccagtc gaaccggcgc
    30001 aaaccccacg atcgaattct gtcccccgtg cgaatcattg gcgagcgtgg tccatcccgc
    30061 gggactcgcg ctgatagcgc tcaccagctc aggcttcacc gcatgttctt ccggtgccag
    30121 aaccggacaa atcaatggaa ccccgtcaga attgaacagc atggcatgcc ccgtctgccc
    30181 caccgtggct tccgacacgg aatgaaacag ggtgtcccgc cgcaaaagca cggtgaccgc
    30241 accgatcgtc acatgctgct catcgtcgat gatcggggcc gacacattga ccacgtgggt
    30301 gccgaaggca gggtcgaaga agatatcgct gacaaacacc ttgcccgttc cgcgcttcat
    30361 gaccgcctgc caccacgccg ccttgccatg gtaatactcg acctgcggga tcgaactcac
    30421 caccagggcg ccgcggttgt ccgtcacaaa aattcccacg tagtcggcct tgcggatatc
    30481 gtgccatcga atcaaatagt tggtcacaat ccggttgatg aacagcggaa actcgctctg
    30541 cttgtcccgt tgacgccacc gctgttgcca ggctttgatc atagactgga cggttttttc
    30601 gtccttgtcc aggtaggtgc gattggattc ggtcaccgag gtgcgcagga agggtgtggc
    30661 ggcgagttgc tgcgcctcgt tgacgccgcg cgtcacctgc atttcaattc gccgggcagc
    30721 ctcgaccgcc acctctttaa agttggcccc gatcgtttcc cgaagcgccc ggcgttcttc
    30781 gatgtaggtg aggacgagtg agaggcacaa cggcaggagg ccgaccatca cgatggccgt
    30841 gatgatcttt cgctggagac tgtgagagcg gctccagaac agcatgggtg cggcttaccc
    30901 cgactaacgg cccccttacg ataatagaca ggcctcagcc caggctcgga cgacccgagc
    30961 ttagcgacgc ttcatgagcc ccccggcact gccgggccac agcagcaaca acgggctcgc
    31021 cacgaagacc gatgaatagg ttccgaacat cacgcccccg agcaatgcca gggagaaatc
    31081 gtgcaacact tcggcgcctg caagcaccag cggaatgagc acgatgacca cggtcaaact
    31141 ggtgacgatg gtgcggctca acacctgatt gatggcctgg ttgatgatcg tctcttcatc
    31201 ctcacgccgt cgcgtccgca aattctcacg gatacgatcg aagacgacga cggtatccgt
    31261 caacgaatac cctgccaagg tcagcaacgc cgtgatcacc agcagcgtaa tctctttatc
    31321 cagcagataa aagacaccca gcacagccag cacatcatgg aatgtcgcca acgcggccgc
    31381 cacgccgaat cgcaactcaa accggaccgc aatgtagagt acgattccga cgaacgaaat
    31441 gaggaccgcg atcatcgcat cttcctggag tttcttcccg atcgtcggac cgatttccat
    31501 gctggaatcc accacaaact tgttggcggg gaattctttg ctgaacactg ccatgacccg
    31561 ttcggcgacc ttctcctcga tcgtcgtcga cgccttcaca cggatgagca acttgttgtc
    31621 ttgagtgaac tcctgcaact ccgccgaccc cagaccgtta ttttccagag cacgtctcgc
    31681 ctcgtcgatc ttcatcgctt gctcgaactt caactgcacc gccgtcccgc cggcgaagtc
    31741 gattcccaga ttcgccgcgc cgcgcgcgat ttgaagcacc gcgatgagac ccagaaacgc
    31801 aaggatgccg gagatcgcaa acgtgataga ccgcttcccc atgaaatcga tattcgtctt
    31861 tcccaaaatc tcgaacatgc cggctccttc ctggcaggat gttgaaaacg tccgccagct
    31921 ttgttctcgc atcgttcaga ggctcaacgt acggcgcaga gtacgcgtcg cctcttcact
    31981 cactacggcc tcgctggacg gcctttttga acatcctgtg agtactgttg cttcgcttat
    32041 gatacagagc gattccctat tcgacgttac cggaacgggt tatcaccact ccgctagata
    32101 ctgagctgct caattttcct gcgctgattg aacaggtcga acaccacttt ggtcccgacc
    32161 agcgccgtaa agaggttgat cgcgataccg aggcagagcg tcacggcaaa gcctttgatc
    32221 ggcccggtac cgaagagaaa cagggcgaac ccggtgatca acgtcgtcac gtgcgagtcc
    32281 acaatcgtca gcaacgcctt gtcgtatccc gcatcgatcg ccacccgaac agccttcccc
    32341 tgccgaagtt cttcgcggat gcgctcgaaa atcaacacgt tcgagtccac acccataccg
    32401 atggtcagaa tgataccggc aatgcccggc agggtcagcg tggcgttcag ccccgcgaga
    32461 gccccgatca gacagatcag gttcagcaag agcgcaaagt ttgcgatcac accggatagg
    32521 cggtaataca gggccatgaa cacgatcaca aggaggcccg caaagagggt agccttcaca
    32581 cctttttcga tggaatcctg cccgagagac ggaccgacgg tcaggtcctg gatgatctta
    32641 agcggtgcag gcaaagcccc ggcgcgcaac acgatcgaca ggttgttcgc ttcttccatc
    32701 gaaaacgtcc cggtgatctg agcccgcccg ccgctgatgc gttcctgaat gaccggtgcc
    32761 gaatagatgg tattgtcgag cacgatcgcc atgcgcttct tcacgttctc gcccgtgata
    32821 cggtcgaact cccgcgcgcc cttcgcatcg aacgtcacgg acacgtaggg ttcattgaac
    32881 tggccgatgg agacccgcgc atcgctcaac acgtcgccgg ccagcatcac ccgctttttg
    32941 accagatagg gcgccaacca ttcctgcccg ctgtcctttt cgacgatccg ctcgaacaga
    33001 atctggtcgc cttccggcac tttcccctcg acctgcttca ggaaggcgtc ctcgcgctcc
    33061 ttgcccttct gaattcggcc gggcaaatcc agcttgagct gattctcatc gtccaacaat
    33121 ttgaactcga gcagcgcggt ctgcttgatg aggtccttgg caagcttggc gtccttgatg
    33181 ccgggcaact ggacgaccac ctgcttgagc ccctgtcgct gaatcaacgg ctctgccaca
    33241 ccgaactggt cgatgcgatt ccggattgtc tccaacgcct gattgatggc ggagtccttg
    33301 atgcgcttga tctccgcatc ccgcaactcc cagacgacct tgtttgcaga cccggccgac
    33361 tccacctcca cataggtcgg gaaatcgtcc aacagcttct ggacctgcgc cttcaattcg
    33421 gcattttgaa atccgatggt gatctgcgac gaaccggtac gtttcaccga ctccgccggg
    33481 actttctttt ccactaacag atcctggagc cccgccgccg tccgctcgac ggcaatctcg
    33541 acggcccgat cttcctccac ctccaacacc aggtgaatgc cgccctgcaa atccaacccc
    33601 agggtgatgc ccttgtccgg caggacctca cgcacccacc gcggcagttc cttgtacaga
    33661 ggttggtatg agggcaagaa aaagatgatc gaggcgacca ccatcgtcac tagggctaac
    33721 aaccgtccgc cgactttttt catacgtgcg ccaatccttc ccgtctccgt cgcgtgcgtc
    33781 agtcttcttc ttcaccgcgc agccgagcga tatgctcttt ctgaattttg attttggtcg
    33841 tgtccgcgat ctggagcgtc accgtgtctt tccccaaatt ggtaatggtc ccccagattc
    33901 cggaggccgt gaccaccttg tcgcccttct taagagcagc cagcatagtg tccgcctgct
    33961 tcctgcgttt ttgctgcggc agaatcagca tgaaatagaa aatgacgaaa atgaggacga
    34021 acggaacaag cgagaggaga ccacctcctg cgcctgtgct ccctgatgtt ccctgcgccc
    34081 atgccaccga atcccacata atgtcccccg cctgtgccgc cgttaagaac cgttcgtact
    34141 ctgagtgtcc gatcgaccat catctgccgg ccgatctaca acatacgtcc gatgaaactc
    34201 acggcgaaac tcctgaaagg ttccgcctcg cacggcgctc cggatctcct ccatcaactt
    34261 cgcgaaaaac cataagttgt gaatcgtatt cagccgggcg cccaacattt cttttacgcc
    34321 gaacaagtgg tgcaagtacg ccctggtgta ccgcgtgcac accggacacc ggcaagccgg
    34381 atcgataggc tgctcatcac gcgcataccg agcctgcttg atcaccacac gcccgaacga
    34441 ggtaaacaac cagcctgtgc ggccgtgacg cgacggcacg acgcaatcga acatatcgat
    34501 cccgcgcgcc accccttcaa ccagatcctc gggcatgccc acgcccatca aataacgagg
    34561 cttggccgac ggcaactcgg gcaccgtgac atccaacatg ccatacatgt cggctttgtc
    34621 ttcgcccacc gacaacccac cgacggcata tccgtcaaac cccaccgaga ccagatcacg
    34681 cgccgactcc acccgcaagc cggcatccag gccgccctga acgatcccga acaacgattg
    34741 atccgtccgc cgtttggcac tgacgcaccg tcgcgcccac agcgacgtcc gcttggaggc
    34801 atcacgaacc tgatccaggg aggacggcaa tgccacgaca tgatcgaatg ccatgatgat
    34861 gtccgcgcct aaggcttcct gaatttcaat ggaggtttcc ggactgataa atcgcgacga
    34921 cccgtcgata tgggattgga acatcacgcc ttcatcggaa atcttgcaaa actttgccaa
    34981 actaaagacc tggaacccac cgctatcggt gagaatagcg ccgggccatg ccgtaaaccc
    35041 atgcaccccg cccaactcct ccaccacttt atgtccggga cgaaggtaca ggtgataggc
    35101 gttattcaag atgagtccgt accccatctt gagcaattcc tctccatcga cactgcgcac
    35161 attgcccagc gagccgaccg gcataaacgc cggtgtcgcg atctcactgc gggcggtggt
    35221 caacacgccg acgcgcgcgc gtccgccgga ttcttcatgg gtgatgcgga aggttaacat
    35281 gggatcggca gtcctacatc tgctctgggg ctgacacgcc caacacgttc aacccgtttc
    35341 ggaccacctg ctgcacggcc cacatcagtg ccagccgggc acccgtcagg cccggcgtca
    35401 tcacctcagt tgcccggcct gaggagccgg actgctcagt ggataaggcg gctgctgcct
    35461 cgctgtctgg tgccggaggt aaaatccggt gcttgttata aaaggggtgc accatgccgg
    35521 ccaactcacg aagatagtag gccattcgat gcggctctaa ctccacggca ctcgcctgca
    35581 acaccaccgg gaacgccgac agtttccgaa tgagcgcgag ttcatcagga tcttccaata
    35641 cattgagatc ggcctgactc ggaaggggac aagcaatgcc ccgcgaagcc gccacacgcc
    35701 ggagactggc aatccgcgca tgcacatatt gcacgtagta gaccgggttc tcttgcgatt
    35761 gctgcttggc taattccaaa tcgaaatcca gatgcgtact ggaatcgcgc atgagaaaga
    35821 aaaatttggc ggcatccgcc ccgacctcgt ccatcacctc acgcagcgtg atgaactctc
    35881 cggaccgctt agacatctcg accttctggc ctccacgaag gagattcacc atctgcacca
    35941 gcacgaccct cagtcgctcc ttcgggtggc cataggcctg cacgaccgcc tgcatcctcg
    36001 gaatataccc atgatggtcg gcgccccaca catcgaccag gagatcaaag ccccgtcgca
    36061 acttatctcg atggtaggcg atgtcggacg ccagataggt atagtccccc tcctgcttgc
    36121 ggaccacccg atccttttca tcaccgaacg ccgacgatcg aaaccattgc gcgccctcct
    36181 gatcgaacaa gaggtccctg gctctcagct cgctcagtac ctgatccacg gcgccggacg
    36241 acaacaacga ggcttcactg aaccaggact caaaggcgat cccgaacatc tccagatcct
    36301 gacgaatgag tgccagtaat tcacgatacg cgaactccct actccgttgt tccgcatcag
    36361 cagcgggaag ggagagcagc gccgcgccct gttccgcctt cacatgctcg gcgactgtgc
    36421 gaatgtaccc gccatgatac ccgtcttcag gaaaggacac cgtctgccca caagcctccc
    36481 ggtaccgcgc cagaaccgac aggcctagca acttcatctg ccggccagca tcgttaatgt
    36541 agtactcact gacgacatca tgtccggtag cgcataagag acggactagc gcttgcccca
    36601 cagcagcacc gcggccatgc ccgacatgca acggcccggt cggattggcg ctgacatact
    36661 ccacgagcac tcgccgtccc tgccccactt ggctgtggcc ataccgctcg ccctgcgctt
    36721 caatctcgcg cagaacatcc atccagagct ctcgcttgac cgtcagattc aaaaagcccg
    36781 gtcgtgcgat ttccgcacga ctgaacaatg catcccgctg ctcgatgtga tcgataataa
    36841 tttgggcgat ttcaaacgga gggcggcgct ccgatgcaga taaagacatg gcgacggtgc
    36901 acgacacatc accccactcg ggccgcttcg gggcttcgag gttgaccatc ggcatctgct
    36961 ccagcttgag cacccctcgt tgctgcgccg atcgaagcgc cccgaccaac gcctgactaa
    37021 ccttgtcttg caccacaccc tgagtcacaa aaacccctct aagtctgcgt ggaaacagcg
    37081 aaaatcaaac tgggaatcta acacaccatg acatgagaga caagatcgcg agttaggtgg
    37141 caggaaccgt ccgtcgctct gacaataaca gtcggcagac ctcaatgatg cgctccggag
    37201 cgatctgaag acacacgcgc tcacggcact ctgccacggc actccaagtc cggcaggtac
    37261 agggcgctcc agatacaatc gacacggcgc gaccaagcgg agcccaacga cgtgaatcag
    37321 taggaccaaa acaagcaatc gtaggaaccg acaaggcagc ggcgaggtgc gtcacgccag
    37381 aatcgtgtcc cacatagagt ttcgcatgcg acagcacccc tgccattgtg gacaaagcca
    37441 gcccacggat gaccggcacg tcgaccttta tagtcgtgag gacgcgctcc acagcctcac
    37501 gatcagacgg gccttccagc aataggggag ccatgtcggc ctggagcaac cactcaatca
    37561 cgggcacgag aagccgtgca tctaaacact tatgggggct cccgctcccc ggatgaatca
    37621 ccaccaccgt gtgccgattc tcttgaatga gtgcctgaag aacgagcctg ctgcgttcgc
    37681 gaatagccgg agagagaacg agtggagtac cgactagctg actagtcccg ctcagttgaa
    37741 tggcatcaag ataccgcgac gcttggtgtt cggcacacag atcggtagat gcagcagatt
    37801 gcaggcagac atactgaaca cccgaggccc gaagtgtagc cgagagggcc ccttctgcgt
    37861 ccggaagcca tccgactgca atatcgcaat tgccaagcca tgccctaaat tctgaacaaa
    37921 ggctgtccgg cccagcccat agtccactca aatatgacga ttcaagaggg aaaactcgat
    37981 caacttcccc agctccacac aagagcgctc ccacggactg tccggccaaa aacgcaatct
    38041 catggtgagg aaactggtgc cggactgcgc ataaagcagg gcgggacaag agcacatcac
    38101 ctagggcccc agggtgaatg actagcaccc cacgcttgct gccgccctgt ggaacctggc
    38161 tcgtcacggt ttgtcagata tcggtgcgtt ggcgagtgct aaatcttcgg gcgtattgat
    38221 attctgaaat gaacgaagac caaccccaag agatgacagg gcattcgctc ctaacaccgc
    38281 aactcgtaat gcttcacttc gatacagtgt ctggatcttc aagttttgac gttcagccat
    38341 cgccttgaga aattcggcgc accgcttgga atacactgcg tgcattggtt ggaactgtcc
    38401 ctcgagttcc gcgacaacga catcggccgt ttcatcacag gagaccatgt aacgaatcac
    38461 ctcggcatga agaaatggca tatcacaggc cacggcaaat atcctcggct gtgaggccgc
    38521 taacagcccg gtatacagac cacccaaact acctgcgttc gggataacat catagacaac
    38581 tcgacagcca cgaacctcca gtgactcgat tggttcagcg aggacaacca ccgtctcaac
    38641 gaaagtatcc attaatagag agagcgtccg gtcaaagaca ctctctccgt tcaccttcag
    38701 gaagcgtttg tcccgcccca tgcgcaggct ctttccaccg gcaatgagga ccccggtgac
    38761 atcgactagg gtgcgctcgc tcatcccaat gtagtcccca aatcctagtc acataaaaaa
    38821 cgagggggtg agtttcccca ccccctcgcg acaccacctc tagctcgagt atcgacttag
    38881 aggaccttac ccttcttctt gttggctcgg cgggtcagca tccagccttc caaaaacacc
    38941 acgccaactg cgataaccgc tgggtaaata tacacttcct gtggaatcgg aggctgcaag
    39001 atcgtgtaat agaaggccga tacggccatc ttccgtcccg catctccgtt gtgtccgtcc
    39061 caggcaaaga agaccatcgg aatgtactta ccgatgtcga accgcgtgta ctcatcataa
    39121 tcatccttgt cgcccttcag cggacgggag atcatgacag tccactgtcc gttcttccac
    39181 tctgacttca acaacttaac tttttcttcg aagtcatcac gttcttcaaa gtcctgatcc
    39241 caaccagtcc ccttaaagga ccggagagaa ccatccgcct cccacttcag aatgtcggcg
    39301 ttgaacttcg catcccccca caaatatcgt ggcttgaacg gggcaggaat ctcttgccac
    39361 ttgatcggga attggaacgc gatgccatcg ttatagacgg cataattgtt ctgagcagca
    39421 gcaattgacc cttcttctcc ggtcttaggc gcttgctcct tcacaccaaa gttctcaaca
    39481 ttgacttgag taggcgccca aggaagtttc ccctcggcga cactcttcgt ccggtcatcc
    39541 cactggatca gatacaccac gtttttctcg ttgtacaacg acttgaccca gatatcatcg
    39601 atacgattga cgaagttacg aggcttgtgg gtgatctgac cgcccatcgc aacataacga
    39661 cggccctgct tcttccacat ctcattctcg ggatcgttcg aaatttcacc ctcgactgga
    39721 gcagaaggaa caacgaagtt gatcttcggc ttgtcggtca aaggatcaat gccaagaggc
    39781 ttaccctcgg catcgcgctc acacaacgag tttacgaagt tcgcaatgct ccaacggtct
    39841 tcaacggacg tgctatccgc gaatgacggc attggcgtcc cgtttacacc cgtggagaag
    39901 gtacggaaaa tattccgcac attataagga tcttggcgac tcccacggaa gttccaacac
    39961 ttgtgccaat cggccggttg aattgaaaag ccccagtcat ctttcaaatt gaaagcattt
    40021 ccatccccac gcccttccac accatggcac tcaatacatt tcttatcgac aatcagctgg
    40081 gcgcccttct taatgctctc ttcagtcggc tcagcaggtt tgatggttcc aagttgaaga
    40141 atcgtctgtg tctcagactg cttgtcagta aactttcgat ccttgaccaa ctgagtcgtg
    40201 acaaacgaca aaacctgaag ccgctgctcc tcggtcaaga taccttccca cggtggcatg
    40261 gcggaaccgg ggagaccatg cgtaaccgta tcgagcaggt cattcttccc agaatcagga
    40321 gtcttctcgt tgtaattgaa caaaggcaac tctccgctcg ctgtgtggcg gattttaaat
    40381 gtgccctggt tgaaatttct cggtcgtggc cacagacgat cagcgcctgg cccgtcgccc
    40441 gcaccgtcca caccatgaca ccagacacac ttggtgaaat agacacgctt cccagcttcg
    40501 atcatctcgg cgggaggagc aggtgccaat tccccttttg caaagccttc aggaagatct
    40561 ccagcatagc ccaatccaac cgcactcgcg gagaggataa gggcccctaa ggctgctccg
    40621 ataatcggcg tcgcctttac gttcatacga agtttgctca ttgttaacct caccacctgt
    40681 tctcccaggt tgtttgtaag attcaggatt agtcccatgt ccgaggatag tatcccgtgt
    40741 gccagtattc gaacaggatc accttccaaa tttcatccgt tgtcaggtgc tgttcccatg
    40801 gtggcataac cgaggcccac gggaatcctt cgttcggcaa accaattccg cccttagcta
    40861 cgcgccagaa aatgaaggtt tcctgcaact gtgcaatcgt tcccgggtcg gtgaagttag
    40921 cgggaatcgg attgaacgca aaggcatgga gaccgcggcc gttgagattg tcaccgtgac
    40981 agaagtgaca attctggaag aatatttcgc caccttcccg aacgtacttc agatagcctt
    41041 cggccttgtc atcccatggg ttggcattgg gtgccattag ccgtcccatc ccctgttcga
    41101 cgatcagctt attgctatac gcctgatcat acttaccctc gagattaacc cggtacggat
    41161 tctgtgacgt ttgcagggtg taggtcttgc cgtgcacctt cgtgctagct ggcggtgccg
    41221 gatgtaccgt ccggagctca atcggctcat ccacactcgg cttcatggtc atgaaggaga
    41281 atccgccgat aagaatcggc aaaagaatga gatacatcgt ccggagtgcc ttatgaaatc
    41341 ccgtgttggc atccagaacg ttgagaattg gctgacgaaa cttcttccaa tcctcctcat
    41401 ttgccgagac ccacatgaat acggctacga acgatacggt tccgtacatc gcacgcacgc
    41461 tgaatggaat cggcggatag atacggaact tgagataggc ttggataaag gcccaaaata
    41521 acacaccctg ccaaaaccgg gggaaggcaa ttcccatgga gttggccaga taactcaggc
    41581 cggtgaatcc agccacaaag atggccagct cactcaatac ctgcatgggc atgtagccct
    41641 caacgagcct gacggtgttc gagggaatca ctccgaagat cattccgatc aacatgagca
    41701 gtcccaggac gccaatcaat gccccgactg acattaatgc tttcatgtcc tcatctccct
    41761 ttcttccgca gtagcaacca gatacccatc gcaactagga ggcctttgga ggctctttcc
    41821 cttcctgcac ctgcgagagg taatctacaa gtttcttgag cgcaccggcg ctaagctttt
    41881 gcccgaacac cttaggcata gtgttgtcgg gaaatccctt cacaacataa gcgctcggct
    41941 caacgatgga ttccatgatg tagtcaggaa cgctcttcgc tttcccctta taatccttgt
    42001 ctttaatgcg tagtggagca ttcgttccct cgaccaactt cggaccaatc gtgccggtcg
    42061 ctccagcgat ccctggaatg gtgtgacaag ccacacactg ccccttggcg aaaatctgat
    42121 cgacaggctc agtcccatca gccatcagag ctgcgccacc acctgcagcc ttgtcaccct
    42181 ctgttggtgg tttcggacgc tcggactctg gaataaactt ctcataagac ttcacaattt
    42241 catcaaagct gggagcgtct cttccctcac gcacatacag ccaggtgtcg acggcagcca
    42301 actcgggaag agacaaagag attggtggtt tatggatgga cggcatcgga ctgaccttgt
    42361 cattggtacc cttcacaccg taaccagcca ccacgaaaca gctcggacat gcatgggact
    42421 cagcaatgta ttcctgagca ttctcggctg ttcctgaccc aggaaatgct tccttttgat
    42481 cggaatcgcg agcagcagcg ttacccttat gatagcgagg atcttcgatc tgggttcctg
    42541 cgcgctctgg gagtccatct aagtttggtg cgcgctcgcc caacataccc ttgtggaaag
    42601 catggcagag cggacactgg cccttaccaa ttgcgccctg aaccttattc tgaccgactc
    42661 caccgaaaat aatcttctcg ccctcgtcag caagctccgt ctgtggcatg ttgctgaagt
    42721 ctttcttttc ttcgattggc gggaatccac cctctacctg agggagccag ttgccatagc
    42781 ccgagagagc ggcggcaata aagaacatga atccgccgat tttcagcaaa gcgctgatat
    42841 tcgtaaaata gagcgccatc atgaatgtca tcgacgtgat caagacgagc gaaaccggca
    42901 aaagcctaga ctcggcaaag aaattcatgg agtaattggc gatagccata aacagaagca
    42961 tcgcgccgat aatgccgatg aacgttttga ataccgcacg tttgtttgca acaggatctg
    43021 aaatagaggc cttaaaataa aacattaggg ccacgagaat ccctaatgca ggccagccca
    43081 tcgctacggc cgctttaatg agttcaatca cagctcaaac ctcctgcagg aggggaaggc
    43141 tccatgctgc gcgagcagca cagacacctc tcctcattga ttcatttctt tcgctttgat
    43201 tagtgaccaa ctgccggcgc tgccgccctt ccagggacag ctgccttcgc ctcaaccggc
    43261 gctttcttgg cagcaagggc tcccaaccaa aatacgaaca taattgaaat ccagaagaac
    43321 agtacgttag ctgaaatcat gttggcggca aatcccaccg tgtgcgtata ggcccacggt
    43381 gaattgtcgc gcataatctc attcacatgc cagaagagcc ggacagagga tcggatgtat
    43441 cccatcagac ccatcatcca cgtgaatgcc gtggccaaca tgatgagcgc gtactgagac
    43501 cgggcagaaa tcttgcccca ctcgatcggc cccatctgct tggcaccctt catcatgacg
    43561 ctgttaagtg caaacatgaa gaacaggcac gacaaggtgg tggccacctg cggaaccgac
    43621 aaaccgacac gtacgtttgc cgggatatag tagccgtaca cagccaacat gatgatgttg
    43681 aggtacgcaa agaagaagaa acagcccatg aagatattgc cgaacttcga ccacgacacc
    43741 gttgaaacct tgttgccccg catataccac acgaagctca acacggttgt tgtgataatg
    43801 acattgatgc cgccattctt ggccgacatg acgccgtagt tacccagcac agggtgctgt
    43861 tgaccaccca tggctttcaa ctcggcagga gtcataacga tggtatgcgg cgtaatgaag
    43921 accagaaatc cgcaagtcag cagaaacacg agatacttga tatagcgctg atacttttcg
    43981 gcaccacgca tccggccgag cgcttgccag agatagtagt tggtgctcag gaacagaatt
    44041 ccgatcatgg ttgcctgaat gataaacaac caagccaaca gtccgcccat caaggtgata
    44101 cccatctgct gccgataggc atagacctcg cgcatcaacc aataacctgc gaatggcaat
    44161 gggatcagga acgccacacc gagagccatc gcgatgtaac ccatccagtc atagtgggcg
    44221 cgctcctcat cggtcttagc cgacaagaaa cgataggccg cataggccgc cacaacacca
    44281 ccaccgaagg ccatgttgcc aagaatgcga tggagattga gcgggttcca caatgccgta
    44341 tggatcacgt gccagatatt accaagatac cgaccctgtt catcaacacc ggcaggagac
    44401 atcatgaaac cgatccatga attcgccagg aacatcaaga tcgtaccgat gacgttcagg
    44461 acgacggaca tactgaggtg aatccacttc aggaacccct ccttcatctt gtcccagcca
    44521 taatagtaaa tgtagagggt tccgctttcg gcgacaaaca tcaatgcata gatatgcatg
    44581 accggcctaa agatacccga gagatactgg aagaaggccg gatagagagt caggaaggta
    44641 aagatcagaa ttccacccaa aatagccgtc agagagtagg ccgtcagact gatcttaata
    44701 aaatcgtagg ccagctgatc ataccgcttg gccatcgcct tatccttggt cacgaccccc
    44761 ataaattcga tgaccataca gaaaatagga acagccaaga cgaagctacc ataatagagg
    44821 tgctgctgat tggcaaacca gagcaggacg cgactctcga agttgtatcg cggatagtcc
    44881 ttctcactgt ccgtcgtctt cggggccggc gggcccgaga caatcccttc cgttttgtag
    44941 taaacgtcct tacccttctc aacctttgcg tcaccgccct ccttcttcac gccggcagca
    45001 ggagcgccgc cgtcgccagc aagggcaggg atcgcaacca gaataggcaa gaggaggagg
    45061 ccgaccatcg cgcccagcgc catgatcgac atcaacttcg tgcgggttac agagcccatg
    45121 ggttacctcc ttgatacatc gcgtatgtga acaataactt ctaaagcccc accctccgaa
    45181 tgattacttt cgaaggagat accaaaagtc caaaccctgc gcatccatca gaaaatgatc
    45241 aacagccgca aaaaacgaga cgcaaatcac cacggatacg acgacataca caattgtttg
    45301 gcccatctca aacccccctc aacgtgataa acaagttccc aggccagaat accggatcag
    45361 caagggcact gaatgccgcc gaaccaatag aaaaaccaaa gacccactgc cagggtgatg
    45421 tagaaaatca ttttcccgat cttaaccttg atgtcatcct gagcagcttc catcgtgagt
    45481 ctccccaacg gtttaagtac gtattttaaa ttgacacacc aactacgctg tgttaatcaa
    45541 aatccctcac gcacatgatt tttaaaggga tttttatggc taaaaattca aaagtgttcg
    45601 acattatagt tttggctgga atggttgtca atatcatttt ggctgtgttt ctgatcctct
    45661 actacttcga tttcctatga ttttatccga ccccattccc ttggatggct ggccattctc
    45721 cgcactcgac tgagcgcaac gaaagccgat tgtttcatcc cgaaaatcca gcatcatctt
    45781 gctgcgcgcg gtaatgcgta aatccgatcc cgaactggta taggcagccc cgcgcagcac
    45841 cttataggtc ccgtattcgg gactttcagg attgcgatca gaggccgcgg cataggcccc
    45901 ctcaagatac caatcgttca cccattccat cacattgccc gcaccatcca tgaccccata
    45961 gggactggta tcgctcttca cccgccccac cggtgccgcc acttcaaacc catcgtccac
    46021 gcgagcccaa ttcgccgcgt tcggcacttc aacgtttccc catggccaca ggcgaccatc
    46081 aggcccgcgc atggccttct cccactccgc ctcagaaggc aaccgccttc cacgccactc
    46141 gcaataggct ttcgcatcct cccaggagac atagaccgca ggctggttca cacctcgcat
    46201 acgcgacgta tttttcgcat agcgtgaggg cggtcctgac tttcgatgcc ccgttgcctt
    46261 cacaaacgcg gcatattgcg cattagtcac ctcgtaacga tcaatcagga accggtcgag
    46321 ataaatcgtc cgctcaggct gctcatcaaa cccgccccgg ttggtcccga agataaacgg
    46381 ccccgcaggg atcgcaacga gttcttcttc aagaggctct tcagcggagg ccgactcggg
    46441 attggagact tcctgatcct gctcggcatg ctggggctca gcctcaaatg gagtggatat
    46501 ggtcccgcga agaatcgcaa tgatgggcaa gctggcggca aacaacacca ccagaagaaa
    46561 aatcactttg aatcgcgttt cgagcatgcc gatatagact acttaacgac cacatcggct
    46621 tcgatgtctt tcgcgcagcg aaagccgacc gtaatgtcat gccgccacgg ttttgcctga
    46681 aaccgcttcg atgaacgcac atttcgcttg gtctcccgcc aggagccgcc acgaataaca
    46741 cgctgctcgc cctggtcagg gcccttcggg tcgcgatacg gagagcctcg ataggaattc
    46801 tcatcgtaga aatccgcgac ccactcaccg acattgcccg tcatatcgta gattccatag
    46861 ggactacgac ctgactcgaa cgaacccggg ggagccaggt aacgaaatcc atcctcattt
    46921 ccatcgatgt tcgcatatcc caccacaaat tcatcacccc aggcatagcg acgcttcccc
    46981 tctccgcgag cagccttctc ccactccgcc tccgtcggca accgcttgcc ggcccatttg
    47041 caataggcct ctgcatcgtc ccacgtcacg gcaatcatcg gatattcagg tttcagtaat
    47101 ttagcggggt cctcttcgaa cacttcgatc tttcgcttga ttcgcttcgt catcttggca
    47161 aagcgatcat aggcatcctg cgtgacctcg tgcctatcca gaaaatatgc tttcagatat
    47221 acctggtgct caggcttttc atccggatca ctatcgttgt cacggctacc catcacaaac
    47281 ggcccttcag aaacctgcac catgtcacgg ccgtcatcgc cgacaagcgt cttatacatc
    47341 gagaagtcct gcgcggcagc cacggcgacc ggcttgacct ccgtggtgat cgaaagcacc
    47401 tgctggcgca actgcttcgc cttataggat tcgtagacca gcccgccaat catcaaaata
    47461 aacgacccga acacaaagat gatcgacccg atcagcacac ccctattttc catctcgtta
    47521 ctcgcacctc aggatttaat aaccgccgag tcagccgaag cctcgtcact actcgcttcc
    47581 agtgccgcag cctttctcgc tgcccgcaca tccaacaaca tcgaaatctg cacgccgaga
    47641 aatcccccca tcgccgcgcc ggcccctgcc ccgaacgaga ccttctcaat tccggcaata
    47701 aaccacgcca acaggccacc gagtaccgta ccgaccagga tactgaccag gaagcggcgc
    47761 ccaccgagaa acccaaccac cgcgcccaac accaatccca atcccgaagc caatggaatg
    47821 gacgagccgc caagaattgc tccgatcaac gcaccgaccg tgcccatcac caccgcacca
    47881 aatgccactt caagcaacac cctggtcgat ggtggattca tccctgccat gatgaccttc
    47941 tacgaaaggg ggttccccca acactcactt gcccgaactc accgtaactg tcccaaaatc
    48001 gatttccaca ccgggccccg caaacaacga aataccccag gccttctcag ccggctcgtg
    48061 cttatgaata cgcttaaaat cttcgtacac gttgacccgc tcttccaccc actccccgac
    48121 cggaagcact ccgctctgca ccaccaattc tgatccgcta aacatcccgc cctcaacgaa
    48181 cgtcccttcc ggctttccgg cgctccagat gtatttcgta aacaccggaa taaacatgag
    48241 atccgtatcc agcgaagcat agagtgccgc aatcggctcc gaaccgctgg gagccttatg
    48301 gagacgccac cgccaggtca gcaccggata gaccttcggg tcccagtcga tcttgcgttt
    48361 cttcacacgc tgccctgcat ccttggccgc aagaaatttc ttcccgtctt gcgattggat
    48421 cttgtaggcg tcgcgcccct tggcctgact acgctggttc tcatgctgcc aatcgatcgg
    48481 aaacccgtcc tgatctgccg actgaaaatc ttccagcacc aacacgccgg cttcgcctgc
    48541 aagggccgga gcgccaactc ccatcacact gcacaatccc accacaccca acagccctat
    48601 tgccgcaaac atcttcgtag ccatctcaca ttccctcatc accttgccta tccctcatga
    48661 gaaggagacg gcagaaagcc tgcctccttc tccgcactca catcttcatg catcaccggc
    48721 tgctgcccgc gctgacagac ccaaaacagg aagaataccg ccccccaaaa cagggccatg
    48781 ttgatcgtga ccatttttgc ggcgaatcca agcgatggcg taaacgccca gggggacgta
    48841 tccggcatca actcattgac gtgccaggcc aaccgaccgg acgagcgaat ataccccatc
    48901 aaccccatca cccaggtaaa cgccgccgcc aacccgaaca agccaaccat tccgcgaacc
    48961 gaaatctggc cccactgtac aggaccgtgg attgttgcac cacgcaacat acgtcggttc
    49021 agcagcaaac ccagcgtgac cacgagcaac gtggtcacgg cctgcgggcc agacaaccct
    49081 acgcggagac tggcgggaag ataaaatcca tacaccgata agccgatgat gtgcgccgtt
    49141 ccgaaaccga acagggcccc caggatgaca tttcccgctt tggcccatga gatcgtcatc
    49201 gtccgatttg cgcgtcgata aaagatgtaa ctcaaggcgg tgatgcagat catcacgttg
    49261 actgcgccgt tcttcgacga catgacgccg tagtttccga tgaccggatg ctgcgccccg
    49321 cccatggctt tgacttccgt cccggtcatc aaaatggtgt gaggcgtcaa ccagataaac
    49381 agacaacccg tcagcgcgag caggagatat tgatagtagg gctgatagcg ctcaccgccg
    49441 cgaatccgtc ccatgctttg ccaaagatag taattcacgc caagaaagag ggcgccgatc
    49501 aacagcgcct gcaccacgaa gagccaggtc agcaacccgc ccatcatcgt gacgcccatg
    49561 ctttgactgt acgcatagac ggaacgcatc aaccaataac cggcgaacgg catcggcaac
    49621 aaggcgcaga ccgtcacaaa caagaacaca tagccgaccc aatcgtaata agcccgctcc
    49681 tcctgactct tgccggaaaa gaaccgataa caggcataag ccaggacgac cgcgccaccc
    49741 gacatgatgt ccgcgaggaa tcgatgcaag ttcaggggat tccagagtgc cgagcgaagt
    49801 aaatgccagg gattccccaa ataccgtccc tgcccgtcga cgccggacgg cgccatcatg
    49861 aaggcggacc aggcattggc taataacagc agcgacgtgc cgaacaccac cgcaagcacg
    49921 ccgatcgaca ggtggaccca cttcgacccc ggcgctgcca tccgatccca actgtagtag
    49981 tacgcgatcg tgagaaacgt cgtcccgaca aacaccagcg catagagagg catcatgacc
    50041 ttgaaggtgc ctcccatgta ttgcatgaag ctggggtaca gcgtgatgaa catcgtcagc
    50101 atcacgctcc ccaccgctgc ggtcacggaa agcgcgagca gcgccacctt caacagatct
    50161 tgcgccaacc cgtcataacg cacggccatc gcgggattcc tcgcgaccaa ccccagaaat
    50221 tccaagagga cgcaaaagat cggtagggcc aggacaaaac caccgaagta ggtatgctgc
    50281 tggatgataa accacatcag caaccggcta tcgagtgacc cgacttgcgg atagtgattc
    50341 tcattcgccg tcggcgccga aggaccgtcc ggaactccag gcgtatggaa atacacatct
    50401 gcggaggggt cggcggccac cagcgatggc ggactcagca gcagaacact cgcggccacc
    50461 agcaacccgg acagcaacaa caggatcacg gacggaagcg gaaggcgttt aatcatgacg
    50521 acgctctctg gctgttcttg tcaaagacag ccgcagatcc actcaatgcc gtaccagcaa
    50581 tggcttcgcg ggacttgccg agaatgccca tgacgaatgg gcagcgcaac aaggcgctgg
    50641 gcactcctcc aggctgacga ttttcgatat cccgccgatg tcccaggtaa tagcagtaca
    50701 gcgccatcac gccaccgacg aggccggcga cgccagcgaa gagtccgggt gaactcatcg
    50761 gctgtttcaa cacatacaga caaccgccga ccaacaccag atagtaggtc gaggcgaagg
    50821 ccagcccggg ccaccaccaa aacttcacca attcactcca gcgggtcgag gacaatccgg
    50881 ccgtccaggc tgccccattt ccttgcccgg cgaaaaacgc caagagaccg accgttcccg
    50941 ccgccatcgt cacgacagcg ccttgcgcca acacggccat gtcaccgcta acgagcgccg
    51001 ccagaagcag cgagagactc acacaaccgg caacccctgc cgccacccac cccgccaggg
    51061 ccggcgtcac tcgcgccagc agggcacgaa gccccgcgcc atcaggaaac gtgacgaacg
    51121 gccgcccgct ggcttcaagc catcgcacat acccgatctc aaatgcatcg cgcgcgaccg
    51181 ccgtactggg aataatcatc gccatcatgg ccgggccatc gggatgcttg gcatagtcat
    51241 atccaacgag ccagagtagc gccagcacga gcagcgacaa ctgcactcca tacacaaaac
    51301 cgatccaggc aagaatccat ccgatacgtc tggctccatc cgcgctcact agttgcgcaa
    51361 acggcacccc tcgaccgacg cgataggcac agatcgccgt caccagcgac accaggccac
    51421 tcaggagcag cagcgtcacc ggctctgcca gcgacatgcc atgcttcgcc gcccggtaca
    51481 ccgcaaacgc caccagccct gggacaaaca tgaccaggcg ctttcttgtt cgaaccgaat
    51541 cttcggtgac ggccagcgtc aaacttggaa ggactcgcag tgccaatcgg tgcaacatgt
    51601 tgtcagtcgc caagcacaat gatgtggggc gcgggagcga cgccgtgggc tccgcgcaag
    51661 agccgcctta gcatttacgc gtcatcgcgc tgagccgcca tgccgaaata tcgcgccttc
    51721 acttcttcca tcagcgcgac ggtcacagag cgttcaccac gatccactgc cgttcgttcc
    51781 aactcaacgc gcgccatggc ccgaaccggc gccggcacat tggcgaggcg ttgctctgca
    51841 tccgggtccc atgagaccat atcgcccgtt ttcgcctgat cgatcaattc gtaggtaatc
    51901 actcgctgcc gtctggtccg ggcaaaccct tccaccagtt cacgcaggac tggaacggca
    51961 tagggaggaa cacgatccag ccgatgccgg gcatcatcag tccacatcag acgatcgacc
    52021 aacccatcga cctgtcccac cggcacatcg acccccacta cccactggca gggccgacac
    52081 tccgaacgga caaaccacgt gggatcgccc tcacgggaga tgcgctcctc aacaccctcg
    52141 gtatgcatcc atccgccgca cccgcacaca agcatatcgg ccctcagctc ttcatctccg
    52201 ctcgacgttc ctacccgatt ttcccgggat agaggatgta caacatggca taggtaaagg
    52261 ttccggtcag aaagaggatg cagtagacga tcatggtgaa acggccgagg gcgcggtgcc
    52321 gctgcccgcc atcaggccaa atccgttgaa tgcccatctc cactgcaaac acgatcgcga
    52381 ccagggtgag aaacccgata tagacctcca gctttcgcat ggaaaacccg gcagacatga
    52441 cgcgtgtgcc gaataacagc atcaccaccg ccaggagcgc cgcaaatatc aggctcacct
    52501 ttttccacga tgtcagcagc cgagcggcac tcagcacccg tgcgccgctc agaaattgct
    52561 gcgaacggaa ccccagcaca atcatgtaca cggccatgac caacccgatg atcaccagaa
    52621 tgatatggat tgtcagcaca gggatgaaca cgtagtcgta tagggcctgg gaacccccga
    52681 atccttcttt tccttcgaac gccaacaccc ccaactgccg aaacagatag taggcggtga
    52741 agaatccgac catggtcacc atcccgccca acatgagcca gtgatggtga tgtccacgat
    52801 gctgcttggc ctgcaaccat ccgatgatga agaggccggt gaacagcgtg gccatcaact
    52861 gactgagatc ggcccccatc gtcgcatggg tgccgaagaa tcccggctga cgcaaaaatt
    52921 caaacatggt acatcttacc cgacacttgg ccgaacacct tgcaccaccg ccgtccgctc
    52981 gatttcctca accgagacat atcccccatc gtgcatccac tgcatggctt ctgcggtgcg
    53041 gtagcaggcg ccttgttcag tattgacgag aatatggacg gcaaacgccg ccgtccaggc
    53101 agggctcgta cccgaggcat ccaagaaccg gtctttgatg atcaagcgcc caccgggatt
    53161 cagatggcta tagattttct tcaccagcgc ggcattggtt gccagatcct gatagtgaag
    53221 gatatcggac atcaacacca cgtcataggg gccacccaac gaatcgcggt tgaagtcacc
    53281 ggccttcagg gaaatcctgc cttcaagccc cgcgtccttc acggtccgtt cggtcaatgg
    53341 gagcgtcgtt tgcagatcga agaccgtcgc cgaaagttgc gggtacacct gacaaaatgc
    53401 aatggcgttg gttccggccc cgcccccgag atcgagcatc gtccgtgcct ggccaagcgc
    53461 aaggcgtttc gccaaatctg ggccactttg ctgcccgatc cggtgcaaga cggacaacac
    53521 attggcgcca agcgcgggat ccgtctcgaa gacatgttgc gtaaccggcg atcggcctgt
    53581 tttcacggcc tcctccagct tgccccaatt gccccattcg gcatcatgca acagcagcaa
    53641 gtgcccgata tattgcggcc cgtgcttgac gagatgcgtc gtggctaccg gcgtattggc
    53701 atagagatcg ccggtcttcg acaaaagcct gatcgccacc agcgcattca gaagtaactc
    53761 cagcgcccgc acatcggcgc cgagtttctc ggccgcttca gaggcggtac ggccgcgtcc
    53821 atccagcacc gaaaagacgt ctaacttcac ggctgtcaaa agaatttttg tttcccagta
    53881 gtaccctaac tggaacactt cggcgagaga caattcacga ggcacagatc acccactcag
    53941 ataatgtggt gttgaaattt ggcatcttac ccagttcaga aagggaaacg caaggcgagt
    54001 ggcgggcagg gaacaacccc tgaaaaccaa taacgacgcg gattggccgg ctggatgatg
    54061 ctgatgaaat cgatggactc agaggccgga acacctgatg tgatgaagag gaagatcgcc
    54121 catggtgcga cacaaacaaa agaggcagtg gtccgatgtg aaccactgcc cctcagtcag
    54181 cgagctatcc cgccgttaaa aacgagacta cttgatttca gccttcaagt tagccgttcc
    54241 accctctgca acttcgactt caaactcgtt cagcttgccc ttgccgacaa agggatgcca
    54301 aaccaccact ttgtgcttac cggccggcac gccagggagt tcgaacgatc cgtcatcctt
    54361 caccactgca tggtacggat tccagacagg gaggaagaag gactgcatga actcatgctg
    54421 gtcacactgc aaacgataat agccctgctt ttccgcgcca ccgcggaata ccacgggctt
    54481 ctctaactta tcgcccttct tcgccaaacc gatgttgaag ccggtggcag agctggagcc
    54541 cttcaccaca aagctatgcg ggttgtgcaa cacacccaag gtcgacttgg gatcatcggg
    54601 gtcggcatcc ttattctccg cacggaagtt cttgttgttc acaaccacgc cgctgaaggg
    54661 caagaattcg cagaattccg ccgtcacgtc cgtgcccttg aatccatcct ggaacgcctg
    54721 atcttcgata tcgaccacgg agacgaccgc acccttcaga ccaccatcct tggcaacttc
    54781 aatggtcttg aggaaacgct tgtcgccatc catgaggctc ttgttcgggt tcttcggaca
    54841 aaacttcggg ttcgggaact tggagaagga gaactccttc tgctcagctt tcccggcata
    54901 agtcactttc cctgcaatcg tgcctccggc aaacgaggcg aggggcgcag ccaaaaacgc
    54961 tgcggccgca acgccgaata ctactttcat cgcacccttc ttcatgtcac tgcctcctta
    55021 gtaatgaaca attaaccgaa actggacacc tactccgtca ccactcaatc acaagggctg
    55081 gtgaccctta ccccttgtat aagacagtcg cattaacttg acgtggctcc acgtcgacta
    55141 cgcttaggcc tctgaacatt tctctatact ggaatcatac tcacactgtc aacttgaaat
    55201 ccagccgcca aagcctccta cgatttcgcg cgattaggtg atggcttcgc atgattgaga
    55261 atgcgcgcaa tcgacccacc cacggtagcc cgaaccgcat cgtcttcatc atgcaaggcc
    55321 aacttcagca tcggcaatat ttccgcccct ccaatctgcc cgagtgccct ggctgcagcg
    55381 atccgagggc gcggaatggg atctttcagc atgccggaaa gaaactcaat cgcagctttc
    55441 atattggcgc catgcgccct ccctgctgcc ttaccggctg cggagcgggt tccgggatca
    55501 tgattttgcg ccaattcgta cagttcctct ccaacggatt caaacggttc ctcgacccgt
    55561 aacagcgccg acacaaccgc cgccttgacc accgggttct gatcagccag cgccttcctc
    55621 agagggacta aggaatcttt cacccccaat tcgcccaggc tgatcgccgc cgacgttttc
    55681 acggctggaa ttttatcctc cagcgcatgc tccaacgcgg ggatcccctg caccttccca
    55741 aggtccccca aggcagatgc ggcggcccct cgcactgatg gctgcgtatt ggtgatggcc
    55801 tccagcagta ccgacagtcc tcgcgggtcc ttcaattctc ccaccatccg tagggctgtg
    55861 gcgcgctctt ccggattcgg ggcggaggcg gccttcagta tcgtgtccca catcgccgtc
    55921 tgccccgtat ggtacagggc tcccgccgcc gcaagccgca ccgccggctg ctcgtccttc
    55981 aaagctttct caaggagcgg gatcaccgag cggtcaccgc tcttccccaa caccttcagg
    56041 acagttgcct tgaccagccc ggcctgatct tccaaggcat tacgcaagcg cggcgaacgc
    56101 cgccccgcct ccagcttccc cagagcttct gccgccaagg cgcgcaccag tccggatccg
    56161 tcacttagtc catcctccag cgcgggaatc atctccgggg aatccacatc tttcagcgcc
    56221 gtgtaggccg cgccacgcat ctgctctcgc atgtctttca ggagcacata cacaaacccc
    56281 aacgccactt ctttcagcag cgcccggtcg tcctgcttca agtcccgctc gagatgctcg
    56341 tactcgacta atgcgtcctt gggctttgct agaagaatca atgaatgaat cttgagccgc
    56401 ctggcctccg gttgaggccc ctgctctttg gtcacttgtt cgacgagatc cagggcttcc
    56461 tgatactgct tcttgtcgaa cgcggcctgc gcctccttca acgaacgcgc ggacgcggcc
    56521 gacggccaga ggcacaagag caccccggtc gccatcgcca ccgccatcca tccactcatt
    56581 gtcagaagcg aacgcgactc attatgcaat gccatgaaat cccttcctgg tcctaccacg
    56641 aggcctatcc tctttcttgt gtcgcggatg ccggagccca gcgtcgatat cccgtcggtt
    56701 cctcaaaata gtagtccggc tgaggggaga gacggatccg cgaatactcg atggaccagt
    56761 cccgatcctg gctcaccaat ttcaagacca cgccggtctc aacatccacc cattcgtaaa
    56821 accgttcatt ccggccgtta tagtccaccc gcacctcgta gagcttggac gcacggccga
    56881 tggtcgtagc atcgcccacc aacgtccggc tcttttcccc cggaaggctg ggctgaatcg
    56941 gcaggatttc ttcgggcttg acgggaaccg gaagaatttg ccggcgctga gccagcaaaa
    57001 accacgactc gtgcttatcc aatcggatga tttctatact ggaatagcca agctcggtct
    57061 tgaccgcgta cttgtactcc agtcggatac gatcgccttt tgcaaaaacc tgagcctcga
    57121 aacgacgccc ttcaacccgc ttcaccagcg aggcagaaaa ctctgccgga ccgatggcag
    57181 atggaccgtc cgccgcagag gcatacccga aaccggacaa acagagaacc cctgagaatc
    57241 cggctcccac gcaccaccgt gggaggcccc cccacctgcg cttagtgctg atgctccacc
    57301 aacggctcaa tctccacggg atgaccaagg gactccagcc cgaaacgagg attgtccatg
    57361 accctgtagg cagtccgagt tcccctggga gcaggaagcg ccacttgttc caccgtagcc
    57421 ccgtcaggac cgaccgtaat cgtccgcgtc accccaggcc ctgcctgcgg atgccagacc
    57481 accatctggt acgtccccgg cgggacattc tcgatcctga acgcgccttg gtcatccgtc
    57541 accgcgtaat aggggttgtt cacggccatc gcccagcttt ccatgtaggc gtgaaagccg
    57601 cactgcatgt aaaacgttcg ccgtcccttg ttcagataga ccggtcctac cagagacttt
    57661 cccggtgcat ggttatgaat cgcatgcata tttccccgtt gatgctgttg atttagaatc
    57721 aagggcgtat tgaacaacac tcgtgccccg gcctcgggag aggtttcata gccctgaatg
    57781 tcgtgcatca ccggatccat attcaccact tccaccgcat ggccattgcg cacgatcgtc
    57841 atccaggggc cgaacatgca atcccgcgcc tcgatcaggg gcaccgagag atcaaatggc
    57901 ttccccgact cgaccccttc gagcagcact acggcatttt tcaaaccgct ctcggcgttc
    57961 acgatgaagt cgcgcaggag acgccacccc ttcccgttcg aaatccgccc acaataggtc
    58021 gggtctggga acgtaatgag attgaacgct ttcgggtccg gaacggcgcc cgacagggtc
    58081 actcgtcctt caagtgaccc gccatgctga acgtcgacaa tgtcataggc agagacggag
    58141 ggcacgacct ggcacaacca gacgacgagc gcaagacaac agagcaggct ggaacgagtc
    58201 aatctcatga tctccctctt tcctcttcga acagacctta gggaacctgg cgctgcagtg
    58261 tcggacgaat atctacgacc tttccaagtg actcagggcc ataatgcgga ttctgctcga
    58321 tttcatgcac gcttcgccgt ccttgcggag ccttgaagtc aaaatcgacg ttcgatagtc
    58381 ccttttccgt cacagtcact ttcttttgca gcatcgtacc gaccccggga tgccagacca
    58441 tcagcgtata gtcgccaggc gggacatccg tcaaactgaa ggtgccatca tcacccgtca
    58501 gcgcgaagta ggggttatcg acggcgaatc cccagctctc catataggca tgaaacccgc
    58561 actgcatcag aaaaattctt cgccccttag tcatatgaat cacttccgta accggctcgc
    58621 ccgccagatg ctcgtgactt tccgcactca caaaacgttt atggtgggga ttcatcggca
    58681 aaggcgtgtt gaacaggaca cgaggaccga gttgcgaggt ctcataggcc tggatgtcgt
    58741 gcatgaccgg atccatgttc ataacagcca cttcagaacc atctttcaca accgtgacaa
    58801 aaggaaggaa ccggcaatca cgcgcctcga tggtcaacgg ttcaaacttg aacggtttcc
    58861 ccttcgtcac atcagtcaag acgacgacga catccttcag accttggctg gaagccattg
    58921 aaaactcatc aagaattcgc caccccgttc cggtcgatat gcgaccgcaa tagaccggat
    58981 cgggaaatgt gatcaggtta tatcccttcg gggttggttt tcctccggtc atggtgactg
    59041 tcccttttat ggtaccgcca tccgtgaccg cgatctcctc gtacgcccac gatggagacc
    59101 agcagacact gacagcgcag agcacggcaa ggccgactaa tcgcatgtca tccctccagt
    59161 aataagtatg atatcgatgc aagcagtatc gacagaaagt cagacgtatc cgctcatcat
    59221 acacaatccc cggcggacaa caaaaggggg gagccctttc aggctccccc ctcctcacct
    59281 catctcttga gagatgcgat cagtctactt ggccgcgaca ggaatcgcgt gcgcaaccgg
    59341 ctgctcaacc tcggcactct tggtgccggc gctcgcgcca gccaatggca gcagtcgcgt
    59401 atcaccggac ggcaataccc gcacatagcc ccactgtccg gactgcgagt acggcaagcg
    59461 ctggttgctg tacacataat ccccgggcaa acgatacatt ccacccgccg aggggaggaa
    59521 cgcgtcgatc gtctcggagc cggagaactc cacgacactg atcaggtcag cgccccgcat
    59581 gaacggctcg atcggccatt cgtgcttctc aacgctgaac atgccgttct gctcgttgct
    59641 gacacccaac acatgaatac gcaccggatc gccggcatgc gcttcgatga gcggggtcgc
    59701 cggatcttcc ggcttgtccg ccttacaagg ttggaacacc ttgccgagcg agcaaccctg
    59761 ctcttcacgg aacttgtacg gttccgaccg gtagttgacg ccggtcaaac ccgccacgtt
    59821 ctgcacgtag ggcatgaagc tcgtgccgat gatgttgtct tcatcctgga agaacaacgc
    59881 cacgtcacga taatttgccc gcatttcatt gcccggcacc gagcgatcga tgatgacatc
    59941 ggccgcccag gcattcttat tggacaaatc cgcaccggtc ttgggatcac gatatttcga
    60001 tccctttggc ccgaccacga cggcaccgaa caacccgttc cgcggattgg tcatcacatt
    60061 gccccaatcc cacaccaacg aggtcgtctc gccgttgaac ggatcggcgt agtacgtgta
    60121 ctcacgctcg gctcccggtg caaccgtctg gtcacccgga ttgttcccta cgttcgcgcc
    60181 catggaatcc ttcggatcga aggccaggcc gatcgcagag aaggaggcct tgctgtcctt
    60241 catcttgttc ttcaaatgca ccttgacgca atcaccgctg ttcacacgca gcgtcagcgg
    60301 catcgggtgt gccccgccgg ccactttcgc ggtgtcttct tccaacgcat agattttcgc
    60361 ttcagcgttg gtcatgagga tccgccgctc aaagtccact tcgatggatt ccggtgcctt
    60421 ggcattgaac ttcatgccgg cgtagtccat cgcgacgacg ttgaactgct tcacgggagc
    60481 atcggcagga cagaccggca gaggcttcgg aatctcccct ttgatcgaga agcccgcggg
    60541 caacttcttc aaatcagcct gctccttatc caacacccgg atgatgcccc agccaccctc
    60601 ggagaacttc gaggaccgac cgttgaagtg aatgtaatcg ccggcttgat gtcgtgatcc
    60661 acccgcctca ggaaccacca ggtcataccg ctcggcgata ccgatatgga tggaattctt
    60721 ccgattggca tccgacgcat accgctcggt ccagaacgtg tggccggaca gggtccagac
    60781 catcgattcg ttcatgaggg tgtgcaacag acggaagacc atcgtgtcac cgacataggc
    60841 acgcaaggtc ggcgtgtacg gatcaccgtg gaccaggctg ctgaaaatct gtgacgaatc
    60901 gggattggtc gccaaccgct gcgcaaccgg cgccgccctg aaattcaagc cgctgccggt
    60961 ggtatgcgta ccaccgttca agaacggcat cggcgtcata tagatcttct cgggcatcat
    61021 gaacgagacg gtctttcctg cctcaagcgc tacttcaatc ggctgcccgg gaggattgcc
    61081 cgccgtcacg atgttgacgg tgtgcggcac ggtgtcgtgc acttggacca tcaattcacg
    61141 gaagctgttg ttcacgccat gaccaacggg ctcgttcgtg tgaatgtccg cgatcggtcc
    61201 gctgcggatc aactttccag tctttggatc atgatacgtc gatccaaccg gttcggcgat
    61261 gaaggtgccg aagccgccgt gcggccaggt ggtcgcacca aacgcgtgat cgtgccagaa
    61321 cacggtcccc acgtccgcat cgacccagaa ccgctgacgc acgaactcga ccgtgacgat
    61381 gtcattggcc gggtgatcgt tcttcagccc tttggccaac gtgatcgtat tgccattgat
    61441 ggccttgatg cgggaaattt cattcccttt cacattgtcg gcaccgacga gaaggagcgt
    61501 gccgacatgg aactgcttgg cattcttcac cgtgatcgtg ttcgcgccct tcttagcggc
    61561 ggcggtcacg acagtgttca tcggggcggg aagccccttc gcatttttct tctccagcat
    61621 cgtgaacgga cgcacggatt gctcatagga gaatccggtg atcacaccgt cggaggcttg
    61681 gttgtcgaac tgcaggaaat gccagtgggt gttgatcttc gacgactgga aattggtgta
    61741 gtcatcgtcg tcccactcgc tggtcaaagt ccagtccacg caatcgtaga tgttgccgcg
    61801 gacaaccagc ggatacttca gatcctcgtt cttccgaatc tccgcctctt cttcgtgcaa
    61861 gacatagatc aagccgttcg gatcgatgac cggcggctcc ttgccgattt t
//
LOCUS       DAORRQ010000021        60329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_27_length_60329_cov_52.585476, whole genome shotgun sequence.
ACCESSION   DAORRQ010000021 DAORRQ010000000
VERSION     DAORRQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 60329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27_length_60329_cov_52.585476"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1157)
                     /locus_tag="PK224_08690"
     CDS             complement(69..1157)
                     /locus_tag="PK224_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB15855.1"
                     /translation="MLCIANAESYFTRLSPAFTRTLGWSLEELMSRPFLDFVHPDDRA
                     NTLQEVERQIGRGEPVLEFQNRYQHKDGSWRLLSWKSSPHPDSRLYAIARDVTDRELA
                     AQALRRSHEELERRVQERTAELQQRNRDLETLLYVSSHDLREPLRSISNFSQLVRERY
                     GTCLDAKGLDYIDRVIRAAQRMDQLMEDLLTLSRAQRMEMPYEEVRGEDLVKAALAQL
                     DETIRRTRARIQVATDFPSFRVNKTWVTQALYNLIGNALKFHRAGEAPEIDLAPYSLM
                     EGSDHRVGLVVRDRGIGVEPEQAQRIFHLFYRAVGREVHGTGAGLAIVQQVAHRHGGR
                     AWVQPREGGGSEFVLLFGSHSTIDTKPT"
     gene            1163..1477
                     /locus_tag="PK224_08695"
     CDS             1163..1477
                     /locus_tag="PK224_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15856.1"
                     /translation="MTARKIDQVLPRSAEAHRDGGRVHRESTAVLDGHAGEVGTVASW
                     ADLSETATVVQDVSAQAERQGSRIGAGGALESCLQELAVHNVVMDVEGSVRSHGGRAS
                     MR"
     gene            1636..2031
                     /locus_tag="PK224_08700"
     CDS             1636..2031
                     /locus_tag="PK224_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15857.1"
                     /translation="MDSRHQRGGRTGVGMFAGFLTGFLWFFLSFAAMFNGVFVLLLFL
                     PPVYALSGALLVWLFRLPPIHAAFSLVGSALPWVAWLWPATISSSGWLKSLVWPALLV
                     TAFGMSVLGAHLVAARRRRKPSQADPTPA"
     gene            complement(2141..2263)
                     /locus_tag="PK224_08705"
     CDS             complement(2141..2263)
                     /locus_tag="PK224_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15858.1"
                     /translation="MVQALSTISDVERVRQYQKLIELELLKMNTFVAAEMTPNL"
     gene            complement(2376..2894)
                     /locus_tag="PK224_08710"
     CDS             complement(2376..2894)
                     /locus_tag="PK224_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15859.1"
                     /translation="MDIISHGLWGAFAFGRRNRTSFSLAFAIGMAPDLFSFGILWVAA
                     TLGLSPTPDFSHGTPPESTIPPYVHYLYNVTHSFVLFLAIFALLWLWTKRPVWELGAW
                     GLHVLVDIPTHSFAFFPTPFLWPLSDWKFDGWQWNQSAILIPNFTVLFLLYAWFLLQQ
                     GRPALRPERVRE"
     gene            3087..3389
                     /locus_tag="PK224_08715"
     CDS             3087..3389
                     /locus_tag="PK224_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15860.1"
                     /translation="MAFNQVGDFWLAPGQSTRVHIALGGLVNEAEWGGHDFGAQWIMA
                     DGVGINPVRLMVSQHTKEKKPIRLHPGSPSPIVYSVTVTNIGEELAHFTIQGGGNV"
     gene            3411..3674
                     /locus_tag="PK224_08720"
     CDS             3411..3674
                     /locus_tag="PK224_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15861.1"
                     /translation="MKAKRTMHVLTDKKGAILGGGVLIPGKDHKGKPVHIRIEPMKGQ
                     SLKEVAIPADLARLEGVEFFRRLQCDFHLPRGKKELLRKAGRR"
     gene            3720..4133
                     /locus_tag="PK224_08725"
     CDS             3720..4133
                     /locus_tag="PK224_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15862.1"
                     /translation="MRPVVSILLGPEGLWIAISLVVYFVAAGNEPSTPAGNEFLETLW
                     LAIPLAGIPLTFLTSYLPGGGGWWWLLRVVVVSSVGLLVASFIAANGVDYHDSRNSGL
                     LGAPVYSLSIGLLLLVPLTIVAAVLVWKMNRARTR"
     gene            4231..4647
                     /locus_tag="PK224_08730"
     CDS             4231..4647
                     /locus_tag="PK224_08730"
                     /inference="COORDINATES: protein motif:HMM:NF018826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HRB15863.1"
                     /translation="MNSDLVLQAARASLTGSLPFPEIVRNLIQAGVEYYHVDYILLQF
                     TFYGTNAASVVAPLTFEGLPAVAKDFDVAALRQTILDSQQDGQQFREFSQRAMRAGVQ
                     GYFAFLKGTRVTYLGRQGDQHTEWFPGAERKANLRD"
     gene            complement(4822..5826)
                     /locus_tag="PK224_08735"
     CDS             complement(4822..5826)
                     /locus_tag="PK224_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HRB15864.1"
                     /translation="MWRSLAATAALTVTLTLPALVVAQEAPATQKSLPEQLVDAFNGV
                     FGVHPGMRANHPKGVVLEGTFTPAASASSVSKAAHLQKKKAPVPVTVRFSAGSGQPNV
                     PDTSQMPRGMAVKFSLPDGSKTDLVVLSFNGFPVATAEEFRDFLLAVGASGPDAPKPT
                     AIEQFLGAHPAAKHFVESPKPQPVSYATLPYFGINSFKFTNAKGAVTFGRYQLRPVAG
                     EHFLTQEQIATAEPDYLSAEIRKRVRRGPAKFKLLLQVAEKDDKIDDPTIVWPDTRKQ
                     IELGTITITKAVADSQTAEKKLLFMPGALVPGIEAADPMIAARSASYIVSLSRRSQAQ
                     "
     gene            complement(6080..7888)
                     /locus_tag="PK224_08740"
     CDS             complement(6080..7888)
                     /locus_tag="PK224_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRB15865.1"
                     /translation="MEAWCKYVNSLTKTGKGKRDFRNRQAAQQSLNKCKDAVPAWVMP
                     LHRVWDTVDPTPGMFDVVIVDEASQCGFEALPLLYLGKKILIVGDDKQISPGGEFQDA
                     TPISKLLDEYLYDFSFKEYFDINVSLFEHGKLRYGTRRITLREHFRCMPEIIRFSNDL
                     CYSDTPLIPLRQYGPNRLPPLEHVFVDGGYREGSHNRTINRPEAETIVEKIAQLCEDS
                     RYDGKTIGVVVLQGEAQAALIEHRLLERLGAEEIERRRLVCGNPYNFQGDERDIILLS
                     LVAATNERIGSLSKAPDERRFNVATSRARDQMILFHSVTCNDLSATCLRRRLLEFFEN
                     KQPRQIAGLERNELERRAAQDNRRLVNPPPPFESWFEVDVALALLRKNFTVLPQYEFA
                     GRHIDLVVEGGRARLAVECDGDQWHGADRYEADMQRQRQLERCGWEFFRVREAAFYAN
                     KENALAGLWGALEERGISPNLPDREPAHEENSEKGDWGTSQMDNAANDDCLDDEDNFP
                     SESPSTDFTPSGRKSEDITPGEIQEAIIQALGKCPNQSCTLHSLTARVLKELGVSTRG
                     KPREAFEKRTMRSVSILETRGRIETYKAKNRRLRLL"
     gene            complement(8076..10994)
                     /locus_tag="PK224_08745"
     CDS             complement(8076..10994)
                     /locus_tag="PK224_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRB15866.1"
                     /translation="MSDQQVCSPESQKAIRLLDYLLRLATLRTKLIRDISEYENVLWI
                     SDVPQERGCYTQAWGRDEEHEPDEWIEVQTRREPELPAVPSQCKDWVNPSALRNKHNL
                     PELRPEITRQIHNPDWSEGSDQPETIPHTERLEDQFVVQQAWDRYLQGKWLPWIEEHN
                     AWEKIHKVYSALFAIHQAQLRLGEEYELVLGLGLLTWQTPNGQRVRRHLVVADVILEF
                     EAGLGKFTIRPHTEGANLRPELDMLDIEDQPTRAEETAKGSLTRAAEDPWETDCIEGV
                     LKALVHSINSQGVYDDSLGPKTVRASTKPLAEFAPAVILRKRSAKGLTETLKRIKEQI
                     ENGQDIPGEFADLAECRPKNDRETGDGHEAPNNSFDGEVFFPKPSNDEQRRIVNKIRS
                     ASGVLVQGPPGTGKSHTIANLICHLLATGNRILVTAKTPRALQVLLGEIDEKEGIQSH
                     EPYKERQRDNGLIPKEIKPLCISLLGSGQEERKSLEASVGGILRKNEEWKPADTDHQR
                     EELEKRLRELREEKARVNRRLRDIRESETHTHSIAASAYCGTAARIAEAVNRDRFTHD
                     WFVDSVPFDKSCQVSTSAMRSILAAVRQFTPANRRELELTWPEGLPSSERFVDLVKSE
                     ARAIEDERNSENGADVRIANMLLKSSSVTIESICDALSAFRDTRKRLLTSSHSWMKEA
                     LRDISSGSSSLWRELLRAMRDVIPAIEKHVAVADNTNLEFPDATDMRTLLDDARTLNE
                     HLENGGSLGWGIFRPRLVKERLYVVQSVKIGGRLCSTGEHFSTLADALYVRIEFEKAW
                     GFWKGRSEQIQGPYTLQLATLKALREALESALALEELIAQSRETLRQCPELGEPVWTD
                     EIHLDRAVSSCRLALARIRKRLATEEIQTIETAISRRAATNDAHPASSDLLKAVRGRD
                     GDGFARCANMIQDLNRQRELLQRVDKDLSELRQLLPKLTKSLEHTCTEPHWGGTTPTY
                     "
     gene            complement(11345..11671)
                     /locus_tag="PK224_08750"
     CDS             complement(11345..11671)
                     /locus_tag="PK224_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HRB15867.1"
                     /translation="MLITQSIALFVLAGLFEIGGGYLVWQWWRNGSHWSIGLLGAVIL
                     ILYGIVPTYQPSHFGRVYAAYGGWFIILSILWGWLVDHVEPDRFDVIGAMVCLVGVTV
                     MMYWPR"
     gene            12168..14066
                     /locus_tag="PK224_08755"
     CDS             12168..14066
                     /locus_tag="PK224_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB15868.1"
                     /translation="MRIEYTKGERASKELILLNRQSFEATSGRKMKVMLIFPPDWFPS
                     EPYLSLPSLTAVLRQAGHQVVQKDINLEMWDWYFSEDFLKKVLRRVPQQLDRLRKLAK
                     KRELEEWEQDLQLTLCDLTRQRIDDLIKKAEKAKAIIRGEVFYEIDQLEWAIHVFREV
                     TSVISMVYVPARICMPPMETDLSYKVFVSHEVMDAVNDTQVNIYRDVFEHLVKPAIEA
                     EQPDVVGISIVLQQQMFSSMTFCALIKQHFPNIHVTIGGNTVTRLRDVLPQSPLFQYF
                     DSAVVYEGETAFVQLVSAVGAKRSLADVPNTIYKDATGVHVSATSFAEDMAALPPPDF
                     DGLPLDKYFVPTKILPYLATRGCYWGRCEFCDHGEGYTAGYRTKKIQDILAEVTYLRD
                     KYGARHFHFTDESYPPALFRKLTRGLIDSKMGIAWTTHMRFEKSLLEDQVWQDAKASG
                     CKYLHFGYESGNERVLKLMDKATTTEIMTKHLKYTAEAGIWNHCMGFFGFPGETREEA
                     WSSVEFLEQNKDYVHSLGFGTFDLGRHNPVAKHPEKFGVTAYKNPEWDLALDYYFTVK
                     QGLSIEEAERVFEEFERNHNPGWDLRLFIREYIFLYIARFGLQKLPDLQFRSAKVATV
                     PPSLAGKM"
     gene            14197..16158
                     /locus_tag="PK224_08760"
     CDS             14197..16158
                     /locus_tag="PK224_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB15869.1"
                     /translation="MSSSGLVQIDGLSPIKNEERKTSKVMLLFPPEWVPTAPYLALPS
                     LTAVLREAGHTVVQRDINIEMYDHFFTMEFLIWVKARLGMQLKPLQDKEKAGTLTEQE
                     ASQKAVLEEAYAVDVFDLADRAEDAKLVVRGERFYEAEKLERALNTFREAMQYISAAY
                     YPASLVFYPMESNLGYRPGVSKEVFACLDDEQVNVYRDICNQLVLPSVSKEKPAVIGV
                     SIGTQMQLMAGLTFCKMIKESFPDIHVVVGGNVVTRLQEEWAKHERFFTEVFDAAILY
                     EGEHALLWYIEAVNGQRAIESVPNLMYYESGAVQQSKEVYTEKTAALPLPDFDGLPLD
                     HYFVPERIIPYLATRGCYWGRCTFCDHGQGYFDQYRGMTAQHVVEQVTALRDKYQCKH
                     FLFSDESYPPALFKKVSQLLVDRNVGIKWTTLIRFEETLQDQAVWDLAAKAGCCTLYY
                     GMESANERVLNLMDKHAKKSVIQNNLHQAAKAGIWNHVMAFYGFPGETRDEALETRQF
                     VIENQPVIHSVELFYFVAYRHTPMVRNPDKFGITIHKQEEYDLPLDYYYTLNEPVGIS
                     CLDAMQLCEEFYKNDFSPWAVRVNSREHVFLYISKFGTNKLPQIYAATKDGATTAEGV
                     SGLVTWPMAIAEGDEGKEGMSRVVSHGVG"
     gene            complement(16516..16770)
                     /locus_tag="PK224_08765"
     CDS             complement(16516..16770)
                     /locus_tag="PK224_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15870.1"
                     /translation="MTEELLTKALELFTLTQPYTRDQLNQRREQLLHTWTPRRFANLT
                     NNPKKYMQAYKKAEEMTRLVEASYALLSTLVAPDDDHRLS"
     gene            16853..18094
                     /locus_tag="PK224_08770"
     CDS             16853..18094
                     /locus_tag="PK224_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15871.1"
                     /translation="MATALPIFQPTPIFKDRTDYRQVLIKEMEAGKIPLSLGRDCPVK
                     CEFCYELDHSYRETLDPPKTSDDDWKFILNYISKKPTDPTQFWCLGGNEFMEWTDLFL
                     HPKAMEWVEDFLRETDKSIQFFTVGFVHVPKIHQLVAQYPGRINFELSVITLSDYRQR
                     LMPHAPSVKHLMKVLDGPAVSSANFYAFDKDTMSKDAATISKVNRQCVLWMGTLTPVR
                     GLKEETAALMRQGRTFLPEEASHVYDLALPNLQTIHTEAYTTAFLSRRRIVSLFDSLE
                     LEKKDTVVMAGSVHKILTMYRKHRARFLYVPNALLSGDSDCTVLLTFDDIARRLTKEK
                     VVHVPKCIMQSGRGPYTDITGVTLEQFSKKTGVRVKVLHKIDTRFANQQLYRNGSLQN
                     YVEQYLKHPLRGAYEAMPRPV"
     gene            18323..19252
                     /locus_tag="PK224_08775"
     CDS             18323..19252
                     /locus_tag="PK224_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRB15872.1"
                     /translation="MPGPRRLQFYQADVFTDQPFGGNPVAVFPDADGLTDVELQQIAR
                     EMNLSETVFVFPPTDKAAVVKMRIFTPTQEIPFAGHPVIGTFFVLATLDRLALREPVT
                     RVLQECNLGLFPVDIHTCNGVIERVVMAQPSPQFLDVIDEPEALFAIARALGITKAVI
                     SETRFPVQVVSTGLPVIIVPVRTLTAVRQIIPDVAAIAELSQQYGANGIMVFSTMTVE
                     QSSSVHTRMFAPLIGIVEDPATGSASGALGAYLVQHGVVDIRPSTEITAEQGYEIDRP
                     SRILIQVDSDDDVIQGVTVGGQAMMVVEGTLTF"
     gene            19541..20572
                     /locus_tag="PK224_08780"
     CDS             19541..20572
                     /locus_tag="PK224_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRB15873.1"
                     /translation="MNAVVFHEHGGPGKLQYQEMPMPTIGVDEVLVRVKACALNHLDI
                     WIRQGSPAYPMPLPHILGSDISGVVHQIGSHVEGVAEGERVYVSPGVGCGRCEHCWAG
                     RDNMCRSYGLIGAMCHGGYAEYVKVPARNVFPIPGALSFEQAAAFPLVSVTAWHMLFG
                     LAAVQPGEDVLIMGAGSGVGHMAIQMAKLAGARVLTTVGSDDKIAKAKTLGADEVINH
                     TREQVNQRVRELTHRRGVDVVIEHIGPEVWGQCVDSLAKGGRLVTCGATTGAEVKLDL
                     RYVYSRQLTIRGSYMGSQSELLKAAQLVGQGRLQPVIDRTFPLSEAKAAQEYLLNRKF
                     FGKIVLSVS"
     gene            20623..21057
                     /locus_tag="PK224_08785"
     CDS             20623..21057
                     /locus_tag="PK224_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB15874.1"
                     /translation="MASIAQIMNKKPQSIGPTTSIRGAAKKMRDLRVGSLLIKKGKNF
                     VGIVTDTDLVRKGLATSQDVSKLTVEKIMTTPLCTIESNQAVDDAQDMMGDLGVRHLA
                     VTKGGSIVGVVSVRDLLMHYKRYAQSRLADKQVYSEPKIGQD"
     gene            complement(21233..21745)
                     /locus_tag="PK224_08790"
     CDS             complement(21233..21745)
                     /locus_tag="PK224_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRB15875.1"
                     /translation="MSSPTAHEHKHHAPRSIGCMVITCSDTRTPETDTSGQLIQQLLK
                     DKGHHIAAYHLVKDEPAEIKARIAEAITNESVQAIVINGGTGISRRDSTFEAVDAMLE
                     KRLDGFGEVFRYLTYQDIGSPAIMSRATAGIIKGRILFSTPGSKNAVRLAMEKLILPE
                     LGHLVKELTK"
     gene            complement(21839..22165)
                     /locus_tag="PK224_08795"
     CDS             complement(21839..22165)
                     /locus_tag="PK224_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRB15876.1"
                     /translation="MPKPKYHILVCTNARPPGHPKPSCGGQGSAQLLMSFNMGLMQRG
                     IMPGEVLVTGSSCLGPCEQGPTVVVYPDNTWYSKVTEADVATILDEHIKGGKPAAKLN
                     PDSVWK"
     gene            complement(22209..22652)
                     /locus_tag="PK224_08800"
     CDS             complement(22209..22652)
                     /locus_tag="PK224_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRB15877.1"
                     /translation="MKFFFYADNLNLSQLKRRAPEHKFLFTAYLPDHSIQFCRWSTQW
                     RCGLASVIPSPGEKVWGAVFEITDEDLKILDDFEGDVPQGAYRHLPVTVITEEGEKLL
                     VTTHAANPIGKFKPKEHYIDWVLKGIKQWKLPEECTEQWKAYKPA"
     gene            22854..23309
                     /locus_tag="PK224_08805"
     CDS             22854..23309
                     /locus_tag="PK224_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15878.1"
                     /translation="MSKVIHYTVLALLLAGGLAYWWMKPPGLNPMLDSQAAEALALVQ
                     THRAAGYPTILQAFNERARIQEARKLGLRLGEWKVIKQDGQRYEVRIYMREQGTTQWF
                     ERDFIWHVDLATKRVNAASLPADGLMPEGPESGRPRAPGGEVPPFPPTM"
     gene            complement(23299..23625)
                     /locus_tag="PK224_08810"
     CDS             complement(23299..23625)
                     /locus_tag="PK224_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRB15879.1"
                     /translation="MDSKPDYITVAQVNQVPPGTCRTVEVEGIFLALCNVDGTFHVVD
                     NTCPHAGGPLGEGCMDGELVECPWHGWRFNVRTGERPENPEIRVACVEVRVQGTNVQV
                     KTPLTL"
     gene            23824..24369
                     /locus_tag="PK224_08815"
     CDS             23824..24369
                     /locus_tag="PK224_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRB15880.1"
                     /translation="MEMTLLLNSTYEPLRVLHWQKAITLLWQGKVEVLEVYDRQIHGI
                     SISIKLPSVMRLLKLVKLKDSHRAVKFSRINIFTRDGYCCQYCKHKFRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNRKSGRTPEEANMRLIKRPVKPRWSPTVTITIGIRNT
                     PESWRDYLYWNLELDADPAET"
     gene            complement(24498..24950)
                     /locus_tag="PK224_08820"
     CDS             complement(24498..24950)
                     /locus_tag="PK224_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15881.1"
                     /translation="MAGYTGRSLAMAGGIIGIAAVAAYFGIAHIGEPDQGGGSIAGQV
                     QIAPNLADQVKSTDVLFVIVRRPTGPPRPIAAKRIDAPKFPVQFEITSADVMVQGTEL
                     KGMVDVIARLDRDGQAGQAQPGDMEGRYPKNPTLPGGKDVNITIDKVY"
     gene            25125..25778
                     /locus_tag="PK224_08825"
     CDS             25125..25778
                     /locus_tag="PK224_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRB15882.1"
                     /translation="MAANPGFQISLDVRGWPVLVIGGDEEAADKVQRLLEAGAKVTVI
                     SPTLHEVLRKLAASAKIIHRGRHFRANDLEAVILVLNTIRDDRELSHSLMRLAREQKF
                     LIWSVDQPDVSNVVMPAVVSSGHVRVAISSSGQAPALSGFMKEDLERILDNEFVAFVD
                     WLGQLREQAKANEPDAEKRRALLREALDGFRLLGKVQYPKVWLDQRAAVGAGEKRGS"
     gene            25828..26136
                     /locus_tag="PK224_08830"
     CDS             25828..26136
                     /locus_tag="PK224_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HRB15883.1"
                     /translation="MVLLEFSMSPLGKGESVSKYVSRSLDIIDKSGVDYRLNPMGTVL
                     EGEWDEVMAVVKKCYMRMRKDCNRVSCSIKIDYRKGPKGRLSSKVSSVESQLKRKLKV
                     "
     gene            complement(26345..27766)
                     /locus_tag="PK224_08835"
     CDS             complement(26345..27766)
                     /locus_tag="PK224_08835"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRB15884.1"
                     /translation="MGGRRRFDRNSLVCFSAAILSLCATAPAAAAGTDTARHQQMETS
                     SMTIHVPIPMTALAEQYASIHQSLAAHRHTHRDWVTLGGGTGFLKYRIWPGEQSSTTT
                     GGRLLSQSSFPFGVEYAKQIKGTVTKIAECGRQDATTGTGRLSVTLATTFSQARAYTL
                     LPVSSVTAVEANRPCLLSEQGVDTAPLMAQVYRSELQGMLPTVDRKAATIVTLKPAVT
                     HIWNDLQEPLLLDEAEGLWLQLNPEAIGPAGVEPLSGTPAAGYGVTARPAVVRGIKPL
                     SRHLPLPDLQSQFHDDGFHVTFALQVPIEEANQRLREAVVGQEWSLGVGTIKIVGATL
                     YPLGNQVGVELALRGLLPLTLRLKGTPAYDEAAGRILFRDVDYTIKERTPATDLAEEW
                     LHEPLRDELAGRLVFPIREELDLMRQALEKGLNRNLTGGRLRGTVHKLALEALTVQTA
                     NLSVRFKTEGTLHYDAHADIGAP"
     gene            complement(27868..29031)
                     /gene="alr"
                     /locus_tag="PK224_08840"
     CDS             complement(27868..29031)
                     /gene="alr"
                     /locus_tag="PK224_08840"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRB15885.1"
                     /translation="MYTPSQFPPTSVSVDLNALAQNLAHVRRLAPRSEVLAIVKANAY
                     GHGALELTRTLQQLAVHRFGVATVDEGIALRQAGIHDAILVMGPTVPAQFPDLIAHRL
                     TPVLYRSDMVQVFAATISPNAAPYSVHVKIETGMGRLGILPQEVPDLVAMPAFRATLR
                     LEGLMTHLADADNTETTHTEKQLAEFQQTLDTLRQRGISCPLTHAANSAGIIKYPASL
                     YSLVRPGIMLYGYHTLPNAAAAPELKPILTWKATIAHLHNIQPGDSVSYNRTFVASRR
                     SRIAVLPVGYADGYNRLLSNRGVVLIGGRRVPVVGRVCMDMTMVDVTDVPGVQVGHET
                     ILIGQQGQERITAADLAAWQQTIPYEVLCAIGQRVPRHYLPLLSADKTSSPKK"
     gene            complement(29058..29621)
                     /gene="frr"
                     /locus_tag="PK224_08845"
     CDS             complement(29058..29621)
                     /gene="frr"
                     /locus_tag="PK224_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRB15886.1"
                     /translation="MSTAQEVKQRVTEKMDHAIEHLKRDLAGLRTGRASVALVDGIKV
                     DYYGTMTPLKQVANVATPEARLITIQPWEQNLIREIEKAIQTSDLGLTPSNDGKLIRI
                     PLPPLTEERRKELIKVCKKHGEEVKVQIRGFRRDGNEELKKLQKDTKLTEDELRKSEA
                     EIQKLTDQYVQKIDDVMKKKEGEILEV"
     gene            complement(29633..30358)
                     /gene="pyrH"
                     /locus_tag="PK224_08850"
     CDS             complement(29633..30358)
                     /gene="pyrH"
                     /locus_tag="PK224_08850"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRB15887.1"
                     /translation="MSSAHYRRILLKVSGEMLAGEQGYGVQPSILEGLAEEIADVVAM
                     DVEVAVVIGGGNIFRGLAASARGMERASADYMGMLATVLNALALQNALEGKGVSTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKKRVVIFAGGTGNPYFSTDTAASLRAMEIGAQVIMK
                     GTKVDGIYDSDPVKNPQAKKYAEIPFLSILTQNLKVMDSTAISLCMDNRLPLIVFNLK
                     EKGNFKRVVQGEPIGTLVTVESR"
     gene            complement(30355..30951)
                     /gene="tsf"
                     /locus_tag="PK224_08855"
     CDS             complement(30355..30951)
                     /gene="tsf"
                     /locus_tag="PK224_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRB15888.1"
                     /translation="MASLSELVKELREKTGAGILDCQKALTENGNSIDKAIDYLRQKG
                     LAAAQKKAGRETNQGLIHAYIHAGGKIGVLIEVNCETDFVARNEQFKAFVNDLALQVA
                     AASPSYVRREEIAEDVVAKERSIYEGQAKELGKPPAAWPKIIEGKLEKFYQENCLLEQ
                     AFIKDPSVVIKDLLAQQIAKIGENMNVRRFTRFQLGQA"
     gene            complement(31003..31776)
                     /gene="rpsB"
                     /locus_tag="PK224_08860"
     CDS             complement(31003..31776)
                     /gene="rpsB"
                     /locus_tag="PK224_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRB15889.1"
                     /translation="MGVVAIKELLEAGVHFGHQTNRWNPKMKRFLFGERNGVYIIDLQ
                     QTVERMEQAYAFARDTVAAGDSVLFVGTKRQAAEILEEEAKRANQFYINQRWLGGMLT
                     NFQTIRRSIDKMKKMEATLADPTHQGHTKKELGQMQKEVVKLQKNLSGIRNMRALPGA
                     VFILDTRIEHIAILEASRLEIPVIAIVDSNCDPDHIQYPIPGNDDAIRSIKLIISRIA
                     DACLEGAHLRTQREETEFASAPAGGGAKPSANLAGVAAS"
     gene            complement(32006..33217)
                     /gene="argJ"
                     /locus_tag="PK224_08865"
     CDS             complement(32006..33217)
                     /gene="argJ"
                     /locus_tag="PK224_08865"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRB15890.1"
                     /translation="MKIITGGITAPIGFRAAGMYCGIKKKQKKQLDLALLVSEQEGPV
                     AGVFTTNQVVAAPVVLDRLHLKKGIGRAILVNSGNANACTGPDGMRVANEMAQLVATA
                     LGSAPHTIFIGSTGVIGQPLPIDCIKNAVPSLLARVTRRGGNEAAQAIMTTDLKPKQV
                     AVRARLGGRMVTVGGMAKGSGMIHPNMATMLGYLTTDAVIGRSALQTALSQAVDQSFN
                     CITVDGDTSTNDTVLCLANGMAGNQTLKPGSADFRRFCAMVETVCRTLALKICWDGEG
                     VTKVVRVEVTRARSTMAAKQIAQTIATSNLVKTALFGGDANWGRVMAALGRAGVPIDP
                     SRVSLQFGDVPMVRNGQGLGRVAEQKLTKVFRAKEFTIRVDLAQGQAEAHMWTTDLSY
                     EYVRINASYRS"
     gene            complement(33273..34346)
                     /gene="argC"
                     /locus_tag="PK224_08870"
     CDS             complement(33273..34346)
                     /gene="argC"
                     /locus_tag="PK224_08870"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRB15891.1"
                     /translation="MTSVRVAVAGGSGYTGAELLRLLSQHPNVQLTAVTSEKSAGTPV
                     STVYPHLQGIVPLTFEALAPEALAERADVLFLALPHTKSMGPVASCMKAGKRVIDLSA
                     DFRLKDPRTYETWYQTTHAHPDLIGSAVYGLPELHRSAIAQARLVASPGCYPTAAILQ
                     LAPLIAHGLIVPDTIVIDAKSGVSGAGRSPALPYHFPEAHESLEPYKIGQHRHIPEIE
                     QELSGLAGTRGQAGNGHASAVTIAFTPHLVPMNRGILSTAYARMKGKLDVVELRALYR
                     DFYKGERFVRLLEGAMPNPRHVRGANYCDLAVHTDTRAGWVVTVSAIDNLIKGAAGQA
                     IQAMNLMLGYPEETGLVAPGIYP"
     gene            complement(34460..34852)
                     /gene="rpsI"
                     /locus_tag="PK224_08875"
     CDS             complement(34460..34852)
                     /gene="rpsI"
                     /locus_tag="PK224_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRB15892.1"
                     /translation="MAAITQYATGKRKSAIARAWVTAAAGDIVVNEKPLEKAFPRMTL
                     RNVIQFPFELAGVAGKYSVRATVYGGGPAGQAGALRHAISKALVVMSAAFRSPLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            complement(34895..35326)
                     /gene="rplM"
                     /locus_tag="PK224_08880"
     CDS             complement(34895..35326)
                     /gene="rplM"
                     /locus_tag="PK224_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRB15893.1"
                     /translation="MTKTYLEKPADVKRKWFLVDADGKTLGRLAAKVATLLRGKHKPT
                     FTPHVDTGDHVVIVNAEKIRLTGNKMEDKTYSYHTGYPGGLKTLTAEHIHKKDPTKLL
                     TRAIEGMLPKNPLGNQMAKKLRVYAGSTHPHHAQQLESLSL"
     gene            complement(35377..35865)
                     /locus_tag="PK224_08885"
     CDS             complement(35377..35865)
                     /locus_tag="PK224_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRB15894.1"
                     /translation="MDSSKQQHEPSELVALTVKQVLDDGNTDTRIVVLKNDDASVTLP
                     IWVGSAEGNAIRLAMERVVTPRPMSHDLIRSFADHLGVRIERVVITDVKGSTYYASVA
                     FASKGVHRTLDARPSDAIALALRADCPIYATQDVLNRRTAVHLDAWINKLDTKNIEPQ
                     QA"
     gene            complement(35869..36324)
                     /locus_tag="PK224_08890"
     CDS             complement(35869..36324)
                     /locus_tag="PK224_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRB15895.1"
                     /translation="MITQMRVKGLIFDPYNNAYIVVLRDEENSDMLPIWVGKSEASAI
                     GLALESVTAPRPMTHDLMKSFLETFDAKIISVVITDLNENTYFATIHLMYEDSEYTVD
                     SRPSDAIALALRTSAPIFASEKVIKKQSSEELEQWLENLKPEDFGKLDT"
     gene            complement(36550..36831)
                     /locus_tag="PK224_08895"
     CDS             complement(36550..36831)
                     /locus_tag="PK224_08895"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HRB15896.1"
                     /translation="MADVQCVTCGQAGEAITDMLFLGKLEAEIKAKVCKPCWKKWEGM
                     RVMVINEYQVNLGDESGRELVKKQMKAFLKLGEQTDTSKLDQNFRPAGS"
     gene            37010..37096
                     /locus_tag="PK224_08900"
     tRNA            37010..37096
                     /locus_tag="PK224_08900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37044..37046,aa:Leu,seq:caa)
     gene            37360..38142
                     /locus_tag="PK224_08905"
     CDS             37360..38142
                     /locus_tag="PK224_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15897.1"
                     /translation="MRNVLALGAAVLFISSVGVAGAQERLPQSSSGSGFGVGTGVGDV
                     SGNSGRTAAFDRTAFMDRLAQAETVFGRVLAVDVPGQKLHLETGGSSHDEGRAGTGAM
                     SSLTLYIDGQTNMDQIRVLNAGDDISVQVVESPNVRADQGVYGTGRKLVREVYLFRGN
                     EKLAGFGGLGQRPNPATERGLETNSGTVVGGLVGGVFPGTVTTGVVTTTVGEFTGQAP
                     CWNCEPQPGWGYQTVAPETKVQNKSDYGTDGAKPNLQKGLTN"
     gene            38290..38550
                     /locus_tag="PK224_08910"
     CDS             38290..38550
                     /locus_tag="PK224_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRB15898.1"
                     /translation="MSDVPLNLQAPDEELDLRGVICPYNFVKTKLKLESMAPGQLLLV
                     HLDDGDPIRNVPRSVSDDGHDVVSQERADQSYRVMIRKRFDE"
     gene            complement(38551..39303)
                     /locus_tag="PK224_08915"
     CDS             complement(38551..39303)
                     /locus_tag="PK224_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRB15899.1"
                     /translation="MGRERSQGKRAQSAPPAGTLFIVGVPIGHPDDLTIRGLATLRQV
                     DLIAAKNPQATHALLAHHGIHTTITTYDRNNAADKVPLLLERLRQGCRVALVSDCGMP
                     VVYDPGRLLITAASRAQIPVKVIPGTSAVVAATALAGMDGNAFVFDGRWTGGTKALTG
                     RLQLLQSEPRTMIFFPPARDLPQFLTLLQQMLGNRKIVLAIDMTRSTEKILRGRVQQL
                     LSRKPGPEQASHVAVVVEGAKSKRKISKRQSR"
     gene            39368..40174
                     /gene="ccsA"
                     /locus_tag="PK224_08920"
     CDS             39368..40174
                     /gene="ccsA"
                     /locus_tag="PK224_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRB15900.1"
                     /translation="MSSVFFMLTMGLYFVSTVCYLAYLLRRAETLSKVSLGITAVGFA
                     THTVALVARMTDTAQAQLPTFHEALSFFSWMLILVFLAVEFRRQLHVLGSFIVPLALV
                     SLVTAAAFRSDEASSLQPVFKTLWVHVTLSMLGTVGFAVAFVAGVMYLIQDGLLKSKR
                     FNVLYAKLPALDYLDHLNQQSIVMGFPLLTLGIISGAFSAEIVRGTYVNWNPEQTWAM
                     VTWGFYFAVLVGRLTIGWRAKRAAYLTIIGFAGVILTLIGVVLKSHGSVS"
     gene            40176..41582
                     /gene="hemA"
                     /locus_tag="PK224_08925"
     CDS             40176..41582
                     /gene="hemA"
                     /locus_tag="PK224_08925"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRB15901.1"
                     /translation="MHVIVVGLSHKTAPVEIREKLAVPESRLREALSRLCAYPGVREG
                     LLLSTCNRVEVYAVVEELETGYGRVQEFLADTHLSLSSEHLTPHLYWHAGDRAIGHLF
                     RVASSLDSMIVGESQILGQIKDAFEVALTHKASGLLLNKIVKKAISVAKRVRTETKIA
                     ETAVSVSYAAVELAKKIFSNLTEKTVLLIGAGEMAKLAARHLIANGVRQVRITTRNFQ
                     HGLELAQRFNGTAVPFEDYKTELAGADIVLVSTGASHYLVTADDVQRAMRQRMSRPMF
                     LIDISVPRNIDPAVRPIDDAFLFDIDDLHMRVEQNREDRLREAAKAEQMVVEEVAVLG
                     QWLQSLEVTPTIVALRSRTEELKRREVDKILARLAHLSSEDRAAVEALASAIVNKMVH
                     GTMVTLKAEAKSSSGAAYVDAARRFFNLEETPAVYPPGQADVSELRGENIAGNGVGRL
                     PEASLVQHTSKEQGRRVS"
     gene            41668..42600
                     /gene="hemC"
                     /locus_tag="PK224_08930"
     CDS             41668..42600
                     /gene="hemC"
                     /locus_tag="PK224_08930"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRB15902.1"
                     /translation="MSRSTLILGTRGSKLAVHQSQWVQARLQELAPGLTISLQRIQTS
                     GDKILDVPLAKIGGKGLFVKEIEDALLSKEIDLAVHSMKDVPTALPEGLDILCVPPRE
                     DPRDALITRDGCRLDQLKPGARIGTSSLRRQAQLLHYRPDFTIEMLRGNLDTRLRKLR
                     EGQFDAIVLAAAGLRRLAWDAEITEYLPVHLSLPAIAQGALGIEARSDDTFVRELLSR
                     FEHRPTRITVTAERALLHRLEGGCQVPIAAHAALEGDRLTVDGLVASVDGRRVIRHQI
                     QGPASEAQALGTKLAERLLADGGDVILKEIYGQA"
     gene            42606..44171
                     /gene="cobA"
                     /locus_tag="PK224_08935"
     CDS             42606..44171
                     /gene="cobA"
                     /locus_tag="PK224_08935"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRB15903.1"
                     /translation="MTTRAGTVYLVGAGPGDPKLLTIRGKECLEQADVVLYDYLANEA
                     LLQHVSPTAERLYVGRRGRGQYRDQAEINRLLIDHAQAGKRVVRLKGGDPFVFGRGGE
                     EAEAVAAAGLEFEVVPGVTAAVAAPAYAGIPVTHRTMASTVTFVTGHEDPTKDRSGVE
                     WRRLATVHGTLVFLMGMTNLPKIVQCLRAEGKEGTTPVALIRWGTRASQRTVVGSLDD
                     IVEKAAAAHMEPPTVIVVGEVVRLREQLNWFERRPLFGKRILVTRPKPQAVEFSDLIA
                     AQGGEPVECPTIEIVPPEDWAPLDRALARLSTYNWLIFTSVNGVAPFMTRLLETQRDV
                     RALSGLTICCIGPRTAEALAAYGLRADVIPEQFQAEGILEVLAARQMRGARVLIPRAL
                     VARELLPDQLRAQGADVDVVPVYRTIRPAVATDRLMEQLRTGGIDVISFTSSSTVRNF
                     VELFTTKDELLRSVGKATVACIGPITAATAREAGLTVQVMAGQNTVPALADAIVDFVV
                     NPIDGRTASAVIH"
     gene            44223..44615
                     /locus_tag="PK224_08940"
     CDS             44223..44615
                     /locus_tag="PK224_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB15904.1"
                     /translation="MVPVKSFMVPKDKFITVERDMDVRSAGRVMRDRNIGSLFVTNGK
                     DVIGILTDTDMVRRVVATGADMTKTTVEQIMSAPLVTIEEHKTLLDANDLMAQTHLRH
                     LGVTKDGQLVGMISVRDLVVFLTNLPRK"
     gene            44623..45606
                     /gene="hemB"
                     /locus_tag="PK224_08945"
     CDS             44623..45606
                     /gene="hemB"
                     /locus_tag="PK224_08945"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRB15905.1"
                     /translation="MAFPIQRLRRLRETEPLRRMVRETSLTPNDFIYPVFVTEGQGRR
                     EPIVSMPGQSRLSIDLLVKEAHEVKALGIPAMILFGIPDRKDERGSSGFDPEGIVQRA
                     IRAVKAQVPDLVLITDVCIDEYTSHGHCGIVRDGKILNDETLDCLRTMAKTHAQAGAD
                     MVAPSDMMDGRVAAIRDELDCAGFVDIPIMAYAAKFASCFYAPFRDAANSSPQFGDRQ
                     SYQMDPANKREALREINLDIEEGADIIMVKPAMPYLDIISAARERTLLPIAAYQVSGE
                     YSMIKAAAQAGWLDERRAMLESLLSIKRAGAEIILTYFAKEAARLLQSRPA"
     gene            45603..46568
                     /locus_tag="PK224_08950"
     CDS             45603..46568
                     /locus_tag="PK224_08950"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRB15906.1"
                     /translation="MKISRGLTPSTSRPYSVVTIGNFDGHHHGHRALLDRVVATARRE
                     AGTALVLTFDPHPVKILAPQVNLMFLTTPEEKLARFEAAGIDEVVFLEFTTAFAALSP
                     AQFAKQVLCDGIGTRELYVGEHFAFGKGRAGRIADLLELGAQLGFRVHPMAPVTIDGE
                     VVSSTRIRQLIQAGELQKAVRLLGRPYSIEGTVISGAHRGTELGWPTANLRLPEGRVI
                     PPDGVYAAQVSWKGSGLNAVVYIGTRPTFGAGERLLEVSILDERLELYGEVIRVDLLG
                     FIREDRVFASAEALTRQIALDVDAARAQLRDAGITASLSALQPRS"
     gene            46572..48056
                     /gene="tilS"
                     /locus_tag="PK224_08955"
     CDS             46572..48056
                     /gene="tilS"
                     /locus_tag="PK224_08955"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRB15907.1"
                     /translation="MTGSQSDLHHPLHKQVARAIRARRLLQPGQKVLVAVSGGPDSVA
                     LLSILHQLAPAWNFSLTVVHCNYGLRGAESDGDASFVAALCRRLELPCHIRRLTVDRS
                     GTGESSSLQARARESRYRLFRDVVAELGADRVALGHTADDQAETVLLRMLRGAGLRGL
                     AGMPHIRESLFVRPLLSRSRQEILAYLQAVGLSYRTDSSNAKSIYLRNRVRHELLPVM
                     QSLAPAAARILARQADLLRGDDRLLDALAAHRLERTIRSRDSATIVLDRTVLLKQPPA
                     LQRRILRQAIQAVAPSTGAPRSDVLLSMLASLASHSGGIWNAGAATVACEQDQVRLTA
                     VSPVPPAGGTVLQPIHTAPVPDVVTIAALPSAVSWPLTGAVIRLRAVTRERGLVLLEK
                     PSSSVALLDADRLSWPLTIRPWCHGDWFFPTGMAGRRKKLQDYFTDAKLGRSMRERIP
                     LVLSGEGIAWIVGQRVDARFAATGSTIRFVLARVTQPLRRKGVF"
     gene            48059..48610
                     /gene="hpt"
                     /locus_tag="PK224_08960"
     CDS             48059..48610
                     /gene="hpt"
                     /locus_tag="PK224_08960"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRB15908.1"
                     /translation="MERIFGRPIVTQEQMRTRIRELGRQIASDYAGKDLVLVGVLKGA
                     YAFYADLARAIRIPMRVEFIVVTSYGAGKKSSGKVKLVSDLTEPIAGKDVLLVEDIVD
                     SGLTVQYLMKTLSRRKPRSLKVCTLLSKPDRRTVEVDLEYVGFKIPNKFVVGYGLDYR
                     QEYRNLPYLAALDQGDEQEQESA"
     gene            48699..50513
                     /gene="ftsH"
                     /locus_tag="PK224_08965"
     CDS             48699..50513
                     /gene="ftsH"
                     /locus_tag="PK224_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRB15909.1"
                     /translation="MNSRVKNLLFWVVVGLFMILLFNLFSVPTHAPEDEVIFSDFMAK
                     LDKGEVMKVTIKANHISAILKDQSRIRTYTAEYPELVKHLREKDVQIEARPPDESPWY
                     ITFLVTWGPFILFLGLWFFLMRQMQIGGNKALSFGKSRARMLTEERKKVTFSDVAGIE
                     EAKEEVLEIIEFLKDPRKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTTEGVILIAATNRPDVLDPALLRPGRFDRQVVVNRPDLRGRSEILK
                     VHTKKVPLAADVELEKIARGTPGFSGADLENLVNEAALWAARWNKKEVELIDFEMAKD
                     KVLMGAERKSMVLSDEEKRTTAYHEAGHALMAKLLPGTDPVHKVTIIPRGRALGVTMQ
                     LPTDDRHNYSKDFLYNNLAILMGGRVAEELVLHDVTTGAGNDLERATDLARKMVCEWG
                     MSEKLGPLTFGRKEEEVFLGREMGSKRDFSEQVALEIDHEVRRLVTENYERAKRILTD
                     NMTSLKALAEALLEKEVLDALEIDQILIQGSSSQTVPA"
     gene            50794..51603
                     /gene="folP"
                     /locus_tag="PK224_08970"
     CDS             50794..51603
                     /gene="folP"
                     /locus_tag="PK224_08970"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRB15910.1"
                     /translation="MGVLNVTPDSFSDGGAYLTVEHALQHARQMQAEGADIIDIGAES
                     SRPGAQPIDETEELRRLLPVLEAVHDAVPLPISVDTTKAAVARRAIRAGATIVNDISA
                     LRGDPLMGSLVAETGAAVVLMHMQGTPQSMQQGPRYGHVVEEVLAFLRERVQVAIAHG
                     IKGSQIVLDPGFGFGKLQEHNLQLLAEFDAFTKLGYPVLAGVSRKQFIGNLTQQPVQE
                     RGYGTAGAVAVAVLKGARIIRVHDVRSMRDTVSVVSAISCHARSHVGVSNA"
     gene            51596..52945
                     /gene="glmM"
                     /locus_tag="PK224_08975"
     CDS             51596..52945
                     /gene="glmM"
                     /locus_tag="PK224_08975"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRB15911.1"
                     /translation="MRKLFGTDGVRGVANLEPMTSEIAMQLGRAAAHIFMRRAGRHQV
                     VIGKDTRLSGYMLESALTSGICSMGVDVLLVGPLPTPAIAFLTRSLRADAGVVISASH
                     NPYQDNGIKFFSNEGFKLPDELEARIEQLIVSDEIKHLRPTADAIGKAYRIDDAEGRY
                     IEFVKRSLPRDLDFQGIKLVVDCANGAAYKVAPAVFRELGAEIEVIANTPDGMNINDG
                     CGAVHPERLQEAVRRHGAHIGIALDGDADRAIFVCEQGKIIDGDHVMAALGLDSHAQG
                     QLARATVVGTVMSNFGLEIAMKKAGIQLMRTPVGDRYLMERMLADGYNFGGEQSGHFI
                     FLDHNTTGDGLISALQILSLMKRTGKPLSELAKAMTAVPQILLNVKVKHKPDLNQIPD
                     IQQAIKSAEVTLNGSGRVLVRYSGTESLLRIMVEGERDSTIREVADHLAEIVRARIG"
     gene            52972..55503
                     /locus_tag="PK224_08980"
     CDS             52972..55503
                     /locus_tag="PK224_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRB15912.1"
                     /translation="MLPTLTIIFVLGLVLGSYLTYFPLSIAIVLLACTAGCVWLERQH
                     RLTSQQSNVLLACLFGGCLYWTLCASFTPHVLLQDSARVLPVRLTGTIVEAVRHAPGR
                     VTAMVQVTGSDDPQLPIPFHLRLTWRDPDRDLHRGLQIRTRIHVHAPSGTLNPRGFDY
                     AAYLDALGVDAVGSVSGAGVIEVLDPDREAPLHLFASLIEEWRGRVRAAAETLPQPSR
                     GLFLSLTIGEQGFLAPEVREWFMTTGTVHILSISGSHLGLIALLSFALVRKACLLLPS
                     ILLLSLSRWLTATRLAALLTLVPVLAYTVLAGAETATIRSAIMIAVALWTVWLGSPHY
                     LLHALAAAAGLTLLVHPAALFDISFQLSYVSVLALALALSRETPVDELPLPKESPAGR
                     MVYWFRESLRLTALVTLATVPLVACYFNQVSWMGLFANLLMVPFVGFIFLPTSLLSAV
                     WVIVTHSSTLPGAAFIDSLGQGLIASTHVLAGLPGAEWFVAAPTIPMMLLFYVLGWAL
                     LAGWPAYAAPLLRRSMASGLACILLWWLWSPRPFGGEEMVRVTFLDVGQGDSAVIELP
                     DGTVTLIDGGATYERFDMGRSVVAPFLWSRGIRRIDHVIGTHPQLDHVGGLAWILAHF
                     QVEKFWTNGAERQEEFWRRIERAMVQRKLEATVATEGRLISEGPACRMVVLNPQPRPE
                     SSISAKSESLNNLSVVTELVCREQRVLFTGDIEREALTRLTHSGNSAHIALLKVPHHG
                     AKSSLERRWLETIRPAVAVVSAGRHNPYGHPAGEVLAAYQAVETQVWRTDRDGAIWAD
                     LDLTRQELSMHSTREWILQPALPSADIWSVEQDNLRRLWRRWNWT"
     gene            55938..57119
                     /locus_tag="PK224_08985"
     CDS             55938..57119
                     /locus_tag="PK224_08985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB15913.1"
                     /translation="MTKRLLFLAPAPPSDRQGGGALRMLHLLRFLGSRFQVDLIAPAL
                     DGAEDAQRQLKDVCAETMFVPQQGPGFLDRIGQVSPYVKDRALAAVVRERLASGQYGA
                     VHLEKPAMIPYLPPHVSVPVVLDVWSYSSTSPLRILRGVGGATGRSRQLVRLIKVGIF
                     DRFRWPMTHCVTVVSDEDRIRCERAHPGQRVLVVPNGVDCRTILPKPDHAATSPLLLF
                     TGDMGAEPNVEAALFLATEVFPAIRRDFPKAELRLVGRNPDPRVRRLAGSGIEVTGAV
                     PDMQLHLRAATMYVAPLCTGTGSRTKILEAMAAGLPIITTSVGIEGMKVQPGRDLLVA
                     DQPVDMIESIHTLLMSQPDRERFGHAARHMAEIWYDWSRCLWPLEPLYQNLLIPKAVA
                     C"
     gene            57316..58377
                     /locus_tag="PK224_08990"
     CDS             57316..58377
                     /locus_tag="PK224_08990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB15914.1"
                     /translation="MIPHSKPSLGQDDIRAATEVLRSGLIAGGPVVGQFERGMAAYLG
                     VQGGVAVNSGTVALELALRAMGVGHGDNVILPSYVCPAPWLAVQRVGAQARIVDIDPA
                     TYNLDPYKVRKARTSRTRAVIVPHLFGLPADLTVLESLGIPLIEDCAQTLGAMEQGRA
                     VGSVGLLTVCSFYATKLLCTGEGGMVLSNDEALLERVRALREYDQAPSLNAAAFNCKM
                     TDLQAAIGVTQLNQLGEFLEKRAALAAVYRDSLPAELFTPPSVPAGRTHVYYRFVVRL
                     QKGLQSSDEFTAYLSRMAHRGLQCRKPVFRPLHRYLELADFPASDEADDVAISLPIYP
                     SLSDDDVKLASQILQEELR"
     gene            58559..59590
                     /gene="mtnA"
                     /locus_tag="PK224_08995"
     CDS             58559..59590
                     /gene="mtnA"
                     /locus_tag="PK224_08995"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRB15915.1"
                     /translation="MVPTVEWKDGAVRLLDQSRLPMHVEFLDCRDYRAVAQAIRDLKV
                     RGAPAIGVTAAMGVALGAQAVTATEYRDFATQVGEICDHLAASRPTAVNLFWAIGRMK
                     QKLAALNNQPVAGIKAELITESQTILDEDIALCKAMGQHGAALIQSGQTILTHCNAGA
                     LATAGYGTALGVIRAAWEQGKQIQVIADETRPVLQGARLTAWELMQDHIPVTLITDNM
                     AGALMRQGKIQLCVVGADRIAANGDVANKIGTYSVAVLARAHNIPFYVAAPYSTIDLK
                     TQSGAEIPIEERNPLEVTAIHGSHPIAPPGVAVLNPAFDVTPSGLITGIITERGVFKP
                     GELAAHFRS"
     gene            59777..60196
                     /gene="ndk"
                     /locus_tag="PK224_09000"
     CDS             59777..60196
                     /gene="ndk"
                     /locus_tag="PK224_09000"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRB15916.1"
                     /translation="MSERTLAIVKPDAVKKNAIGDIINRYEKAGLRPVAMRLMHMSKA
                     TAQGFYAVHKARPFFDSLCTFMSSGPCVVLVLQGDNAIKKNRELMGATDPAKAEQGTI
                     RAAHGANIEFNAVHGSDAPETAKFEIGYFFAEMELVG"
BASE COUNT        11709 a        16866 c        18394 g        13360 t
ORIGIN      
        1 cctggcgggt cgcggtcagt cagtcggctt gattttcaag acagttgctg accccgattt
       61 ggtgtgcatt acgtaggctt ggtgtcgatg gtcgagtggc tgccgaacag cagcacaaat
      121 tcagatccgc cgccttcccg tggttgcacc caggcccggc ctccatgccg atgcgcaact
      181 tgttggacaa tggccagtcc cgcgccggtg ccgtgcacct cgcgccctac cgcccggtaa
      241 aacagatgga agatccgctg tgcctgttcc ggttcaaccc cgattccccg gtcccgcacc
      301 acgaggccga ccctgtggtc ggagccctcc atgaggctgt aaggggccag gtcgatctca
      361 ggggcctcgc cggcccgatg aaacttcaac gcattcccaa ttaaattgta gagcgcttgc
      421 gtgacccagg ttttattgac gcggaacgag ggaaagtcgg tggccacctg gatgcgtgct
      481 ctcgtgcgtc gaatcgtctc atcgagttgt gccaatgctg ccttgaccaa gtcctcgcca
      541 cggacctctt cgtagggcat ctccatgcgt tgtgccctcg agagtgtgag cagatcctcc
      601 atgagctgat ccatccgttg tgccgcccga atcacgcgat cgatgtaatc gaggcccttt
      661 gcgtccaggc aggtgccgta tcgttcgcgt accagttgcg agaagttcga gatcgagcgc
      721 aggggctcac gcagatcgtg agagctcacg tacagcaagg tttccaaatc acgattgcgc
      781 tgctggagtt ctgccgtgcg ctcctgcacg cgccgctcta actcttcatg ggaccgtcgt
      841 aacgcctgcg ctgccagctc acggtcggtg acgtctcgcg cgatggcata gagcctgcta
      901 tccgggtgcg gggaggactt ccacgacagc aatcgccagg agccgtcttt gtgctgatag
      961 cggttctgaa attccaggac cggttcgccc ctgccgatct gacgctcgac ctcttggagc
     1021 gtgttggccc ggtcgtcggg atggacgaag tcgaggaacg gtcgactcat caattcctcg
     1081 aggctccagc ccaatgtgcg cgtgaatgcg ggactcaggc gagtgaaata gctctccgcg
     1141 ttcgcaatgc agagcatgtc caatgacagc tcgaaaaatc gatcaagttc tgcctcgctc
     1201 ggccgaggcg catcgggatg gggggcgggt gcaccgtgag tcgacggccg ttctggatgg
     1261 tcatgcggga gaggtaggga cggtggcgtc atgggcggat cttagtgaaa ccgctacggt
     1321 cgtgcaagac gtctctgctc aggcagagag gcaaggttcc cgcattggcg ctggaggggc
     1381 gttggagtcc tgtttgcagg aactcgccgt gcataatgtg gtgatggacg tcgaaggatc
     1441 ggtcaggtcg cacggcggac gtgcttcgat gcggtagcca aagcctccct ccagggcctc
     1501 gccggcagcc gctgcctggc gcgaagtgtc gaaggcacgg gatgaacgaa acagtcgggc
     1561 cgcgcgcatg cgaagaaata ggcgtcgaac gtaaaactcc ttggtgctct tccggtccgg
     1621 agctacactg tgcccatgga cagccggcac cagcggggcg ggcggacagg cgtgggaatg
     1681 ttcgccgggt ttctgacagg cttcctctgg ttttttctca gtttcgcggc catgtttaac
     1741 ggcgtgtttg ttctcctgct tttcctgcct cctgtgtatg cgctatcggg agcgctcctc
     1801 gtgtggctct tccgcctgcc tccgattcat gcggcgttct ccctcgtggg gtcggcgtta
     1861 ccctgggtgg cctggttgtg gcccgcaacc atctcaagct ccggttggct gaagtcctta
     1921 gtatggccgg ccctccttgt gacggctttt gggatgagtg tgctcggtgc gcacctcgtg
     1981 gctgccagac ggcgtcgtaa gccgtcccag gcggacccga ctccggccta gcgcttcatc
     2041 ggtggggccc cgcaatcgtc cgctgcgcgt tctcgggatg tctcgtccgg gcgtccttca
     2101 gcactcgcat catgagtccg ggcatcgtca caccggcagc tcacaggttg ggcgtcattt
     2161 cagcggcgac gaacgtgttc atcttgagca gttccagctc gatcagcttc tgatactgcc
     2221 ttacccgctc tacatctgag atcgtcgaga gcgcttgcac cacgtcattc cagtccggcg
     2281 aaatgagtgc gagcgagagg ttcggtctgc ggatctgtgg tcgatgcgaa ggagggaaat
     2341 ggctggctgg gacgtgggat cggaatcagg tcagttcact cgcgcacccg ttctggcctg
     2401 agggcgggac gtccctgctg caggaggaac caggcataga gcaggaacag caccgtgaag
     2461 ttcgggatga gaatggctga ctgattccac tgccacccgt cgaatttcca atcagagaga
     2521 ggccagagaa agggcgttgg aaagaaggcg aaggaatggg tgggaatgtc gaccaacacg
     2581 tgcaagcccc aggcgccgag ttcccagacc ggccgcttcg tccagagcca gagcaaggca
     2641 aagatggcga gaaacagcac gaaactatgg gtgacattgt agagataatg aacgtagggc
     2701 ggaatggtgg attccggcgg tgtgccgtga ctgaaatcag gcgtcggcga cagacccagt
     2761 gtggccgcca cccacaggat gccgaacgag aacagatccg gcgccatccc aatcgcaaag
     2821 gcgagggaga agcttgtgcg gttgcgacgt ccaaacgcga acgctcccca gagaccatgc
     2881 gagatgatat ccatgcgctg tctttcctca cgggactttc cgcgacctca ttatagtcgg
     2941 aggcctcacg acgaggctac ttcgcgacgt tcccaatgac ggaagatcgc tgcagagtcg
     3001 gaacgatcat gtccgacggg aattcggatt cccctgcgca tacccgttcg ctataatgcg
     3061 cccgaactct tggagggagg tgctttatgg catttaatca ggtcggagat ttctggctgg
     3121 cgcccggtca atccacgcgt gttcatatcg ccctcggggg cctggtgaac gaggcggaat
     3181 ggggcgggca tgatttcggt gcacaatgga ttatggccga cggcgtcggg atcaatccgg
     3241 tgcggctcat ggtcagccag cacaccaaag agaagaagcc cattcgcttg catcccggtt
     3301 cgccaagccc catcgtctat tcggtgaccg tgacgaacat cggggaggaa ttggcccatt
     3361 ttacgattca aggcgggggc aacgtataga cgttccgaag gaggtcgcca gtgaaagcca
     3421 aacggaccat gcatgtgttg acagataaga agggtgcgat cctgggggga ggcgtgctca
     3481 tccccggtaa ggatcacaag ggaaagccgg tgcatatccg gatcgagccg atgaaagggc
     3541 agtcgttgaa agaagtcgcg ataccggcgg acctcgcaag gctggaagga gtggagttct
     3601 tccgccggct gcagtgcgac tttcacctgc cgcgcggcaa gaaagagttg ctccggaagg
     3661 ccggacgacg ctagacgagt gtcctcgtcg cggagccagg gtgccgcccc gaggtggtga
     3721 tgcggcctgt cgtctctata ctgcttgggc ccgaaggact gtggatcgcc atcagccttg
     3781 tggtctattt cgttgcggcg gggaatgaac cttcgacacc tgcaggcaac gagttcctcg
     3841 agacactctg gcttgcgatt ccgttggccg ggattcccct cacatttttg acgtcctatc
     3901 tgccgggagg gggcggctgg tggtggctcc tgcgcgtggt ggtcgtttcg tctgtcggct
     3961 tgctggtcgc ctcgtttatc gctgccaacg gggtcgatta tcacgattcc cgaaactccg
     4021 gcttgctcgg cgcgcccgta tacagcctct caatcgggct cttgctcctg gtgccgctca
     4081 cgatagttgc tgccgttctc gtttggaaaa tgaaccgggc aaggacgaga taacggtgcc
     4141 tggtgtgagt ttctacggcg gtagggagaa tgtgtgattg caagacctga cccgatttgg
     4201 agagtttttt tgctctgaga aggaggccgc gtgaattccg accttgtact tcaagccgct
     4261 cgtgccagcc tcaccggctc gctgccgttt ccggagatcg tgcggaatct tattcaggcc
     4321 ggtgtcgagt actaccatgt ggattacatc ctgctgcagt tcacattcta cgggacgaac
     4381 gccgcaagcg tcgtcgctcc gctgaccttc gaagggttgc cggctgtcgc gaaggacttt
     4441 gatgtggccg cattgcgcca gacgattctg gatagtcagc aggacgggca gcagtttcgt
     4501 gagttctcac aacgggccat gcgagccgga gtgcaaggct actttgcgtt tctgaagggc
     4561 acacgcgtga cctatcttgg caggcaaggg gatcagcaca ccgagtggtt tcccggtgcg
     4621 gagcgcaaag cgaacctccg cgattgaatg aacgtccggc tcgtgacccg taaccgggtg
     4681 gcgactcaat caagccggtt gagcagggga acgcctgagt atctacgaga gcagatctct
     4741 tgagcggacg gcgagagtga tgcggctggt gcgagtcgcg cgagggttgc cctgcgcctc
     4801 aattcagcta tcccgccgag ctcactgcgc ctgagaccgc cgcgaaaggg acacgatata
     4861 ggacgccgaa cgggcggcga tcatcggatc agctgcctcg ataccgggaa ccagcgcccc
     4921 tggcatgaag agcaacttct tttctgcggt ttggctgtcc gccacggctt tggtgatggt
     4981 gatcgtaccc aattcgatct gcttgcgagt atccggccac acgatcgtag gatcgtcgat
     5041 cttgtcgtct ttttcggcca cctgcaggag caatttgaac ttggcgggac cacggcgcac
     5101 gcgtttacga atctcagcgc tcagataatc gggctccgcc gttgcgattt gttcctgggt
     5161 gaggaagtgc tcgccggcta cgggtcggag ttggtagcga ccgaatgtga cggcgccttt
     5221 cgcgttcgtg aacttgaacg agttaatccc gaaatagggc agcgtggcgt aactcaccgg
     5281 ttgtggcttc gggctctcca cgaaatgttt ggcagccgga tgggcgccta gaaactgctc
     5341 aatggctgtg ggtttcggag catcgggtcc gctcgcaccg accgcgagca gaaagtccct
     5401 gaactcttcc gccgtggcca cgggaaatcc gttgaacgaa aggaccacga gatccgtctt
     5461 ggacccgtcg ggcagcgaaa atttgacggc catgccgcgc ggcatctggc tggtgtccgg
     5521 gacgttcggc tgccctgagc cggcagagaa ccgcaccgtc accggaaccg gcgctttctt
     5581 cttttgcagg tgagcggctt tgctcaccga ggacgccgaa gcagcagggg tgaacgtgcc
     5641 ctccaagacg acgcccttcg ggtgattggc gcgcattcca ggatggacac cgaacacgcc
     5701 gttgaaggcg tcgacgagtt gttcaggcag cgatttctga gtggcgggtg cctcttgcgc
     5761 cacgacaaga gcggggagag tcagtgtaac ggtgagcgcg gcggtggctg cgagtgatct
     5821 ccacataggt gtgtccctcc tcgaaggctg tggattcggc gatgcacaac tgcctgcacg
     5881 ctcacccaat cacatctgaa gaccaatggc gaggctggtt gggagcgtgg tgaaaaccct
     5941 actcctccat ggcgaatgag tcaatcgggc aacgagactg aatgaggtgg aaggggaagg
     6001 ggaaggagtg atccgccgcg cattggaaaa tatgtgattg caaacctgac cccatttcat
     6061 tggcgcatgt cgtttcctgt tagagaagcc tcaaccttcg attttttgct ttgtaagtct
     6121 caatgcggcc acgcgtctca aggatgctga cggagcgcat ggttcgcttc tcaaatgctt
     6181 cacggggctt cccacgcgtt gacacgccga gctctttaag gacgcgagcg gtaagcgaat
     6241 gtaaggtgca agactgattg gggcacttac ctagagcctg aataatagct tcttgtatct
     6301 cccctggggt tatgtcttcg gatttccggc cagatggcgt gaagtcagtg cttggactct
     6361 ccgatggaaa gttatcttcg tcgtctaggc aatcgtcatt tgcagcatta tccatttgcg
     6421 atgtgcccca atcccctttt tctgaattct cttcatgggc tggctcccta tcaggtaagt
     6481 tcggggaaat gcctctttct tccaacgctc cccataaccc tgcaagcgcg ttctctttgt
     6541 tggcataaaa agctgcttct cttacacgga agaattccca accgcatcgc tctaactgtc
     6601 gctggcgttg catgtcggcc tcgtagcggt ctgccccgtg ccactggtcg ccatcgcact
     6661 ccacagccaa gcgcgcccgc ccgccttcaa cgacgaggtc aatatgccta cctgcgaatt
     6721 cgtattgggg aagcaccgtg aagtttttgc gcaggagtgc aagggcaacg tcaacttcga
     6781 accaactttc gaatggtggg ggaggattca caaggcgccg attgtcctgt gctgctcgcc
     6841 gttcaagttc gtttctctcc agtccggcaa tttgtcgtgg ctgcttattt tcaaaaaact
     6901 ccagcaatcg ccgccgaagg caagtcgcgc tgaggtcgtt acatgtcact gaatggaaga
     6961 gaatcatctg atcgcgagca cggctggttg caacattgaa gcgtctttca tccggggctt
     7021 tggagagaga gccaattctt tcgttggtgg ccgcgactag cgacaggagt ataatatcgc
     7081 gctcgtcgcc ctggaagttg tagggattgc cgcagaccag gcgccgccgc tctatctcct
     7141 cagcgcccaa tcgctcaagc aatcgatgct caatcaatgc cgcttgtgcc tcaccttgaa
     7201 gcaccactac tcctattgtc ttgccgtcgt accggctgtc ttcgcacagt tgggcgatct
     7261 tttcgacgat cgtctcggct tctgggcggt taatcgtcct gttatgcgac ccttcgcgat
     7321 atccaccgtc cacaaaaacg tgctccaacg gtggtagccg attcgggcca tactgcctta
     7381 agggaatcag tggtgtgtcc gagtagcaaa gatcgttgct gaaacgaatg atttccggca
     7441 tgcagcggaa gtgctcccgc aacgtaatac gtcgagttcc atagcggagt tttccatgtt
     7501 caaaaaggct aacgttgata tcgaagtatt ctttaaagct gaaatcatat aaatattcgt
     7561 ccaacaattt gcttattggc gtagcatcct gaaactctcc accgggactg atctgtttat
     7621 catctccgac gattaagatc tttttcccga gataaagaag tggtaatgct tcaaacccgc
     7681 actgcgaagc ctcatcaact atgactacgt cgaacatgcc tggcgttggg tctaccgtgt
     7741 cccagacgcg gtgcaaaggc atcacccatg cgggaacggc atctttgcac ttattgaggc
     7801 tttgctgagc tgcctgacga ttacgaaagt cgcgtttgcc tttgccggtt ttggtcagcg
     7861 aattgacgta cttgcaccag gcctccatgt gtcggcgatg aggctcttcc attcgcttaa
     7921 aacagaaaga ccacgcatga agtgccgcga gcttggcaac gacgctgttg atttcctcct
     7981 cgatctgttt tgcgcgcttg gcgagcgccg gtgcgtcttc ctgccgaatg tagtcttcta
     8041 tccaatagcg tgcctgcgcc cattgccagg cagcgtcaat atgttggagt cgttcctccc
     8101 cagtggggtt cggtgcaagt gtgttccaag gactttgtaa gcttcggtaa caattggcgt
     8161 agttcagaga gatctttatc cacccgctga agcagttctc gttgcctgtt caaatcctga
     8221 atcatatttg cgcaacgagc aaatccatct ccgtcgcgac cgcgaacggc ctttagcaaa
     8281 tcactgcttg caggatgtgc atcgtttgta gcagccctgc gtgaaatcgc agtctcgata
     8341 gtctgtattt cctctgtggc aagccgtttg cggatacgag ctaaagcgag gcgacatgat
     8401 gagaccgccc tatcaaggtg gatttcgtcg gtccatacgg gctcgcccag ttctggacat
     8461 tgtctcagcg tttcacggga ttgggcaatg agttcttcca gagccaaggc actttcgagc
     8521 gcttcacgta gcgctttgag ggttgctagt tggagagtgt atgggccttg aatttgttca
     8581 cttcgtcctt tccaaaatcc ccatgctttc tcgaattcga tgcgtacata tagcgcgtcg
     8641 gcaagagttg agaaatgttc gcccgtggag caaagacgcc cacctatctt gacagactga
     8701 acgacgtaca accgctcctt cactagcctg ggtcgaaaga tgccccaacc caaactgccg
     8761 ccattctcca agtgttcgtt cagagtacga gcgtcgtcaa gcaacgttcg catgtcagta
     8821 gcatctggga attcgaggtt ggtgttgtcc gcgaccgcaa cgtgtttctc gattgccgga
     8881 ataacgtctc gcatagcgcg cagaagttca cgccacaggg atgaactgcc gctcgagata
     8941 tcgcgtaagg cctctttcat ccaagaatga gagctcgtca acaatcgctt ccgagtatcg
     9001 cgaaaagcgg acagcgcgtc gcaaatcgac tcgatggtca cggaactgct ttttagcaac
     9061 atatttgcga tgcgtacatc ggcgccattt tccgagttcc gttcatcctc tatggcacgg
     9121 gcctcactct ttacaaggtc cacaaaacgt tcacttgatg gcagtccctc tggccatgta
     9181 agctccaatt ctctgcggtt tgccggcgtg aattggcgta cagccgctag gatgcttcgc
     9241 atggcgcttg tggacacttg gcaagatttg tcaaaaggaa cggaatctac aaaccagtca
     9301 tgggtaaatc ggtcgcggtt tacggcttca gcgattctgg ccgctgttcc acaatacgca
     9361 ctcgctgcta tcgagtgcgt atgagtttcg gattcccgga tgtcgcggag gcggcggttg
     9421 acacgagcct tctcttcgcg gagctccctg aggcgcttct caagttcctc tcgttgatgg
     9481 tcggtgtcgg ccggtttcca ctcttcgttt ttgcgaagta tgcctccgac actcgcttct
     9541 agcgacttcc tttcctcctg cccgctgcct agtaggctga tgcacagcgg cttgatttct
     9601 tttgggatca ggccgttatc tctttgacgt tctttatacg gttcgtgact ctggatgcct
     9661 tccttttcat cgatttcacc caaaaggacc tgcaatgctc tcggcgtctt tgcagttaca
     9721 agtatgcgat tcccagtcgc cagcagatgg cagataagat ttgcgatggt atgggatttt
     9781 ccggtaccgg gcggtccttg caccagtaca ccgctcgccg atcgaatttt attcacaatg
     9841 cggcgttgtt catcgttcga tggtttgggg aaaaagacct ctccatcaaa gctattgttt
     9901 ggggcttcgt ggccatcgcc cgtttcgcga tcgttcttgg gacgacattc cgcaaggtct
     9961 gcgaattcac cgggaatgtc ttggccgttt tcaatctgct ccttgatccg ttttagggtt
    10021 tcggtgaggc ctttggcgga acgtttgcgt aaaatcacgg cgggtgcaaa ttcggcgagt
    10081 ggtttcgtcg atgcgcggac ggttttgggt cccaatgaat cgtcgtaaac accttgcgag
    10141 ttgatcgaat gcaccagggc cttaagtacg ccttctatgc aatctgtttc ccaagggtct
    10201 tcagcggctc tggtcaatga acctttcgcg gtttcttccg cgcgagttgg ctggtcttcg
    10261 atgtcgagca tatcgagctc aggccgcagg ttggcacctt cagtgtgggg gcggatggta
    10321 aatttcccca agccagcttc aaattccaga atgacgtcgg caacgactaa gtgccgtcgg
    10381 actcgttgcc cattgggcgt ttgccaggta agtaagccca ggcctagtac gagctcgtat
    10441 tcttcgccaa ggcgaagttg cgcttgatga atagcgaaaa gtgcagaata tactttatgt
    10501 atcttttccc aggcattgtg ctcttcgatc cacggtaacc acttgccttg aaggtagcga
    10561 tcccatgctt gctgaacaac gaactgatct tcaaggcgtt ccgtgtgtgg aatcgtttcc
    10621 ggctggtcag atccttcaga ccagtcagga ttatggattt ggcgtgtgat ctcaggacgg
    10681 agttccggta aattgtgttt gtttcgaagt gctgaagggt tcacccagtc tttgcattga
    10741 gacgggaccg ccggcagttc tggctcgcgc cgagtttgca cctcgatcca ctcgtccggt
    10801 tcatgttcct catcacgtcc ccatgcctgc gtgtagcagc ctcgttcctg cggaacgtct
    10861 gagatccaaa gaacgttctc gtattcggaa atgtcgcgga tcagctttgt ccgaagtgtc
    10921 gccagcctta ataggtaatc gagaaggcga atcgccttct gcgattctgg actgcacacc
    10981 tgctggtcgc tcaagaggtc acctgtgcca gtgggtttat tggtctaagc ggataagtag
    11041 agggcgtcgc tttggctcgt ctcttggggc accctattgg gcgttgtctt ttcttgtgaa
    11101 atcaatgagc tcagcagaac agggcattcc ttcctacgcc ttgcaaggta gtaaatcaat
    11161 cgtaaaatgt tggctgaagt gtctgtggaa ttgtatccat tttttgtagg ccaatggctg
    11221 tcagagagat ggaatgtggc ctggcgggtt ccctacgtgc gcgcgtccaa cgaggggagt
    11281 cgccgaccgc gcgttgcgcg agcaagggga actgccaggc cagcctcttc ctcttcccgt
    11341 ctttctatct cggccaatac atcatcaccg tcacgcccac caagcacacc attgccccga
    11401 tgacatcgaa tcgatccggc tccacgtgat cgacgagcca cccccacaga atcgacagga
    11461 tgatgaacca gccgccgtag gcggcataga cccggccgaa gtgcgacggc tgataggtcg
    11521 gcacgatgcc gtacagaatc aagatgacgg cgcccaggag gccgatgctc cagtggctgc
    11581 cgttccgcca ccattgccag actaaatatc cgccgccgat ttcgaacaga cctgccagca
    11641 cgaataaggc gatcgattgt gtgatgagca tgcgattctc caataaaggg gatcgactcg
    11701 tgctcacata gcacggtgga gcgaaggaga caaatgcagc cgcactgcct gacgcttacg
    11761 tcatatactt cactctccca ttcccagggg gcggcctgtg tggtctccta actgcgcgcg
    11821 tccaacgagg ggagtctgcg accgcgcggt gcgctttcca cgcaccctca ccggaatagc
    11881 cgagttaccc ttcaacttcg cgcatgggac gagcacagtt ttaccgtgcg cgtgttcttt
    11941 ctcacaccat tcccatctgg ggttggcctg gttggtctcc taactgcgcg cgtccaacga
    12001 gggccttcta aggccgcgcg ttgcgcgagc acaggagacc atcaggccag ccccatcctc
    12061 ccactcttgc atctccatct tttcccctcg tacaatgtgc tcctccaagc taaccccttg
    12121 gcattctgac tgtttcggag tgccggaccg atacaggagc cgccttcatg cgcatcgagt
    12181 atacgaaggg cgaacgggct tccaaagagc tgatcctcct caatcgccaa agtttcgaag
    12241 ccaccagcgg ccggaagatg aaggtcatgt tgatcttccc gcccgattgg tttccttccg
    12301 aaccctatct cagtcttccg tccctcaccg ccgtgcttcg ccaggccggt catcaggtcg
    12361 ttcaaaaaga catcaacctt gagatgtggg attggtactt cagcgaggac tttctgaaga
    12421 aggtcttgcg ccgggtgccg cagcagctcg accggctccg caagctggcg aagaaacgcg
    12481 aactcgaaga atgggagcag gatctccagc ttaccctctg cgatctgacg cgccagcgga
    12541 tcgacgacct gatcaagaag gcggagaagg ccaaggcgat cattcgcgga gaagtcttct
    12601 acgaaatcga ccagttagaa tgggccattc atgtattccg tgaggtcacg tcggtgatct
    12661 cgatggtcta tgtcccggcg cggatctgca tgccgccgat ggagaccgac ctctcctata
    12721 aagttttcgt gtcgcatgaa gtgatggacg cggtgaacga cacccaggtg aacatctacc
    12781 gcgacgtgtt cgagcacttg gtgaagccgg cgatcgaggc ggagcagccg gacgtggtcg
    12841 gcatctcgat cgtcttgcag cagcagatgt tttcctccat gaccttctgt gccctcatca
    12901 agcagcactt tcccaacatc catgtgacca tcggcggcaa cacggtgacg cgtctgcgcg
    12961 atgtgctccc gcagtcgccg ctgtttcaat acttcgacag tgccgtggtc tatgagggtg
    13021 agacggcgtt cgtgcagctg gtgtcggcgg tgggggcgaa gcgaagcctg gccgatgtgc
    13081 ccaacaccat ctataaggac gcgaccggtg tgcatgtttc cgcgacgagt ttcgccgagg
    13141 acatggccgc attgccgccg ccggacttcg acggcctgcc gctcgacaag tatttcgtgc
    13201 cgaccaaaat tctgccctat ctggcgacgc gcggttgtta ctggggccgc tgcgagttct
    13261 gcgaccacgg cgaaggctac acggctggct accgcacgaa gaagattcag gacattctgg
    13321 ccgaggtgac ctatctgcgc gataagtacg gcgcgcggca ttttcacttc accgacgagt
    13381 cctatccgcc ggccctcttt cgcaagctga cgcgcgggct gatcgacagc aagatgggca
    13441 tcgcctggac cacgcacatg cggttcgaga agagcttgct tgaggatcag gtttggcagg
    13501 atgcgaaggc ctccggctgc aaatatctcc acttcggcta tgagtcgggg aacgagcgcg
    13561 tgctgaagct gatggacaag gcgacgacga ccgagatcat gacgaaacac cttaagtaca
    13621 ccgccgaggc gggtatctgg aaccactgca tgggcttctt cggttttccc ggtgaaacgc
    13681 gggaggaggc ctggtcgtcg gtggagtttc tggagcagaa caaggactac gtgcattcgt
    13741 tgggattcgg gacgttcgac ctcggccggc acaatccggt ggcaaaacat ccggagaagt
    13801 tcggcgtgac tgcctacaag aatcccgagt gggatctggc gctcgattat tacttcaccg
    13861 tcaagcaggg gctgagtatc gaagaagccg agcgagtgtt cgaagaattc gaacggaatc
    13921 acaaccccgg ctgggacctg cggctcttca tacgggaata catcttcctc tacatcgcgc
    13981 ggttcggatt gcagaaactg ccggacctgc agttccggtc ggcaaaggtc gccacggtac
    14041 cgccgtcctt agccggaaag atgtaacgaa ggcgtgagac gtgaaaggcg aaacgtcaaa
    14101 cgggaaacgt aacatgcgag aatcgaatgt tgaacggcag gtagccctct ttaggccctc
    14161 tcacctttta cgttttgcgt ttcacggaag acgactatga gtagctccgg tctggtccaa
    14221 atcgacggct tgtctccgat caagaacgag gagcggaaga cttccaaggt catgctcctg
    14281 ttcccgcccg agtgggtgcc gacggcaccc tatctcgcct tgccctcgtt gaccgccgtg
    14341 ttgcgggaag cggggcacac ggtggtccag cgcgacatca acatcgagat gtacgatcac
    14401 ttcttcacca tggagttttt gatctgggta aaggcccgcc tgggcatgca gctcaagccg
    14461 ctccaggaca aggaaaaggc cgggacactg acggagcagg aagccagtca gaaagcggtg
    14521 ctggaagagg cctatgcggt agacgtgttt gatctcgcgg accgggcgga agacgcgaag
    14581 ctggtcgtgc gtggcgaacg attctacgag gccgaaaagc tggagcgggc gctgaacacg
    14641 ttccgcgaag ccatgcagta catctccgct gcctactatc cggcgtcgct ggtgttttat
    14701 cccatggaaa gcaacctggg ctaccggccg ggggtctcaa aggaagtctt tgcctgcctc
    14761 gacgacgagc aggtgaacgt ctaccgcgat atctgcaatc aactcgtgtt gcccagtgtg
    14821 agcaaagaaa agccggcggt gatcggcgtc tccatcggca cgcagatgca gctgatggcc
    14881 ggcctcacgt tctgcaagat gatcaaggaa agttttcccg acatccacgt cgtggtcggc
    14941 ggcaacgtcg tgacgcggtt gcaggaagaa tgggcgaagc atgagcgttt cttcaccgag
    15001 gtgttcgacg cagctatcct ctacgagggc gagcatgcgc tgctctggta catcgaggcg
    15061 gtgaacgggc aacgcgcaat cgagtcggtg cccaatctca tgtattacga gtccggcgcg
    15121 gtgcagcaga gcaaggaagt ctatacggag aagacggcgg cgctgccgct accggatttc
    15181 gacggtttgc cgctggatca ctatttcgtt cccgagcgga tcattcccta cctcgcgacg
    15241 cgcggctgtt attggggccg ctgcaccttc tgcgatcatg gccagggcta cttcgatcaa
    15301 taccgcggga tgacagcgca gcatgtggtc gagcaggtca cggcgctgcg ggataagtac
    15361 cagtgcaaac atttcctgtt ctccgacgaa tcctatccgc ccgcgctgtt caagaaggtg
    15421 tcgcagctgc tggtggaccg gaacgtgggc atcaagtgga cgacgctgat ccggttcgaa
    15481 gagacgctcc aggaccaggc ggtgtgggac ttggcggcga aggcgggctg ctgcacgctc
    15541 tattatggaa tggagtcggc gaacgagcgc gtgctgaatc tcatggacaa gcacgcgaag
    15601 aagagcgtga ttcagaacaa tctccaccag gcggcgaagg cgggcatctg gaaccacgta
    15661 atggcgtttt acggattccc cggcgagacg cgcgacgagg cgctggagac gcggcagttc
    15721 gtcatcgaga accaaccggt gattcattcc gtggagctgt tctacttcgt ggcctaccgc
    15781 cacacgccga tggtgcgcaa cccggataaa ttcggcatca cgatccacaa gcaggaggag
    15841 tatgatctgc cgctggatta ctactacacg ctgaacgagc cggtgggtat ttcctgcctc
    15901 gatgcgatgc agctctgcga agagttctat aagaacgact tcagtccctg ggccgtgcgg
    15961 gtgaatagcc gcgagcacgt cttcctctat atctccaagt tcgggacgaa caagttgccg
    16021 cagatttacg cggcgacgaa ggacggcgcg acgacggcag aaggtgtgtc tggtctcgtg
    16081 acctggccga tggccattgc tgaaggggat gaggggaaag aggggatgag tcgcgtcgtc
    16141 tctcacggcg tcggctagca ggcagactgg tgaaaacgcc atcttccggc gttctcggga
    16201 gcgtgtctcc ctgcgacgta ccgcaagggt acgcctcagt cgccacgctc cctgcggcct
    16261 tggcatatga cgttttgacc agcctgcgag atgcggattg taccggatgc tgaaaacgac
    16321 ttcgcacgtc gttctcggtt cgtcaacatc ctcaacgtac ccctgagggt acgcctccgg
    16381 ttgtgactca cctgcggctt agtgggatgc cgtgttgagc atcctgctgg aatgagcgcg
    16441 tggtgcttga aacgtgaagc ctgtctcgcg agatacaaac gacgagaggc gagcgacggt
    16501 tttctccgag acgcttcacg agagacggtg gtcatcgtcg ggagcgacta acgtagagag
    16561 caaggcgtac gaggcttcga cgagccgggt catctcctca gccttcttgt aggcctgcat
    16621 gtatttcttg ggattgttgg tcaggttggc gaagcggcgg ggcgtccagg tgtgcaggag
    16681 ttgctcgcgc ctctgattca gttggtcacg ggtatagggt tgcgtgaggg tgaagagttc
    16741 cagggctttg gtgaggagtt cctcggtcat gtaacggagt ctggcaagtc gataatgacg
    16801 ctgtcaatct agcaggcgtt gcccctcgcg gcaacaggca aggtgttcca ccatggccac
    16861 tgcactcccg atttttcagc ccaccccgat cttcaaggat cgcacggact atcgacaggt
    16921 gctcatcaaa gaaatggagg cggggaaaat tcccctgagc ctgggccggg actgcccggt
    16981 caaatgcgag ttctgctacg aactcgacca ctcctaccgc gaaacgctcg acccgccgaa
    17041 aaccagcgac gacgactgga agttcatcct caactacatc agcaagaaac ccaccgaccc
    17101 cacacaattc tggtgcctgg gcggcaacga gttcatggaa tggaccgacc tgttcctgca
    17161 tcccaaggcg atggaatggg tggaggattt cctccgcgag acggacaaga gcattcagtt
    17221 tttcacggtc gggttcgtcc atgtgccgaa gatccatcag ctggtggcgc agtatcctgg
    17281 ccggatcaac ttcgaactgt cggtcatcac actgagcgac taccggcagc ggttgatgcc
    17341 gcatgcgcct tccgtgaaac atctcatgaa ggtgctggac ggtcctgccg tgtcgtcggc
    17401 gaatttttac gcgttcgaca aggacacgat gtcgaaggat gcggcgacga tctccaaggt
    17461 gaatcggcag tgtgtgctgt ggatggggac gctcactccg gtgcgcgggc tcaaggagga
    17521 gacggcggcg ctgatgcggc aggggagaac attcctgccg gaggaagcca gccacgtcta
    17581 tgatctggcg ctgcccaatc tccaaacgat ccacacggag gcgtacacca ccgccttctt
    17641 gagtcgccgg cgtatcgtca gcctgttcga ctcgctggag ttggagaaga aagacacggt
    17701 cgtgatggcg ggcagcgtcc acaaaatcct gaccatgtac cggaagcacc gcgcccggtt
    17761 tctctatgtg ccaaacgcct tgctgagcgg cgattccgac tgcacggtgc tcctgacctt
    17821 cgacgacatt gcgcgacgtc tgactaagga gaaggtggtc catgtgccca agtgcatcat
    17881 gcagtcgggg cgggggccct ataccgacat caccggcgtg acgctggagc agttcagcaa
    17941 gaagaccggc gtgcgggtga aggtgttgca caagatcgat acccgcttcg cgaaccagca
    18001 gctctaccgc aacggatcgc ttcagaacta tgtcgagcaa tacctcaagc atccgttgag
    18061 gggtgcgtat gaggctatgc cgcgcccggt ttagcagggt agccggctgg tgaaaacgcc
    18121 atctcccgtc gttctcggtc gcatgtctcc ctgcgacgta ccgcaagggt acgcctcagt
    18181 cgccatgctc cctgcggcct aggcacatag cgttttgacc agccgccaaa ttcctactcg
    18241 ctcacctgct tcgctcagtc gccctcttgc aaaaaatacc cgctcggggc aacatgccgc
    18301 caacatcttt cgtgaggaat ctatgcccgg tcccaggcga ttgcagttct atcaagccga
    18361 cgtgttcacg gatcagccgt tcggcgggaa tcccgtcgcc gtgtttcccg atgctgatgg
    18421 cctaaccgac gtggaattac agcagatcgc ccgcgagatg aatctttccg aaaccgtgtt
    18481 cgtgtttccc ccgaccgaca aggccgccgt cgtgaagatg cgcatcttca ccccgacgca
    18541 ggaaattccc tttgccgggc atccggtgat cggaacgttt tttgtgctgg ccaccttgga
    18601 ccgtctcgcg cttcgggagc cggtcacacg ggtgctccaa gaatgtaatc tcggcctctt
    18661 ccctgtggac atccacacct gcaatggcgt gatcgagcgc gtggtcatgg cgcagccgag
    18721 cccgcagttt ctcgatgtca tcgacgagcc ggaggcgttg tttgcgattg cccgcgcctt
    18781 gggcattacc aaggctgtga tttccgagac ccgtttcccg gtgcaggttg tttctacggg
    18841 gttgccggtc atcatcgtgc cggtgcggac gcttacggcg gtgcggcaga tcattcccga
    18901 cgtcgcggcc atcgcggaac tgtcccagca atatggcgcc aacggcatca tggtgttcag
    18961 cacgatgacc gtcgaacagt cctccagcgt gcatacgcgg atgttcgccc cgttgatcgg
    19021 catcgtggaa gatcccgcga ccggaagtgc gagcggggcg ttgggtgcct atctggtcca
    19081 gcatggcgtg gtggacatcc ggccatcgac cgagattacc gccgagcagg ggtacgagat
    19141 cgatcgcccc tcccgcattc tgatccaggt ggactcagac gacgatgtca ttcaaggcgt
    19201 gacggtcggc ggacaggcca tgatggtggt ggaggggacg ctcacctttt agccggatga
    19261 tgaagacgcc atcccactgc gttctcagtc gtacgtctcc ttgtgacgtt agcagtataa
    19321 gagggcgcgc tgaaggggtt tatccgttcg ccaagagcat ctggttggcg aacgggtcaa
    19381 acaagtttgg tatgtacctc ctcagtcgcc gcactccctg cggccttgtt atatggcgtt
    19441 ttgatcatct tgctggaaag ctgtatctcg ctatcctccg cggaaagagc gcattgaggc
    19501 ggaaacgcaa tacagccgcg actcgcgagt gaggagtgat atgaacgcag tggtgttcca
    19561 cgaacatggc gggccgggga agctgcagta tcaagagatg ccgatgccga ccatcggtgt
    19621 cgatgaagtg ctcgtgcggg tcaaagcctg cgcgttgaac catctcgaca tctggatccg
    19681 gcagggcagt cccgcctatc cgatgccgct tccgcatatc ctcgggtccg acatctccgg
    19741 cgtggtgcac cagatcggct cgcacgtgga aggggttgcc gagggcgagc gcgtgtatgt
    19801 gtcgcccggc gtcggctgcg gtcgctgcga gcactgttgg gccggtcgcg ataacatgtg
    19861 ccggtcctac gggttgatcg gggcgatgtg tcatggcggg tacgcggagt atgtgaaggt
    19921 gccggcgcgg aatgtgttcc cgattcccgg cgcgttgagt ttcgagcagg cggcggcgtt
    19981 tccgctggta tctgtgaccg cttggcatat gttgttcggg ttggctgcgg tgcagcccgg
    20041 tgaagacgtc ttgatcatgg gggcgggcag cggggtgggg cacatggcaa tccagatggc
    20101 caagctggct ggtgcccgtg tgctgaccac tgtcgggagc gatgacaaga ttgcgaaggc
    20161 caagaccctc ggcgccgacg aggtcatcaa tcatacccgc gagcaggtga accagcgggt
    20221 gcgcgagttg acgcaccgcc gtggtgtgga tgtcgtgatc gaacacatcg gcccggaggt
    20281 gtggggccag tgtgtggatt cgctggcgaa ggggggccgc ctggtgacct gcggggcgac
    20341 gacgggcgcg gaggtgaagc tcgatctccg gtatgtctat tcacggcagc ttacgatccg
    20401 gggctcgtac atggggtcgc agagtgaatt gctcaaggct gctcagctgg tcggccaggg
    20461 ccggttgcaa cccgtgatcg accggacgtt tccgctctcc gaagcgaaag cggcgcaaga
    20521 atatcttctg aatcgaaaat tttttggtaa gatcgtgctg agcgtttcat aaccggtttc
    20581 attagtggct ctcttgtccc aacgggcaga aaggtatgtg acatggcgag tattgcgcag
    20641 atcatgaaca agaaacccca gagcattggt ccgacgacgt cgattcgcgg tgcggcgaag
    20701 aagatgcgtg acttgcgggt cggttcactc ctgatcaaga aggggaagaa ctttgtgggg
    20761 atcgtgaccg acacggatct cgtccgcaag ggtctggcga ccagccagga cgtatcgaaa
    20821 ctcacggtgg aaaagatcat gaccactccg ctctgcacca tcgagagcaa tcaggcggtc
    20881 gatgacgcgc aggatatgat gggcgatctc ggtgtgcgcc atcttgcggt gaccaagggc
    20941 ggttcgatcg ttggcgtggt gtcggtgcgc gatctcttga tgcactacaa gcggtatgcg
    21001 caatcgagac tggcggataa gcaggtgtac tctgaaccga agatcgggca agactaggcg
    21061 gatgttgaaa atggcctcca gcagcgttct cagtcgcacg gctccctgca gcctcgttgg
    21121 atgaccgttt ggagcactcg tcactggccg gggatagagc gtcaaaagta aaacgtcaaa
    21181 cgtgggatgt gaagaggtga tcgtttcacc tttcacctct cacgtgtcgt tcttatttcg
    21241 tgagttcttt gacgaggtgg ccgagttcgg ggaggatcag tttttccatc gccaggcgaa
    21301 cggcattctt tgatccggga gtcgaaaaga ggatacggcc cttgatgatg ccggcggtgg
    21361 cccggctcat gatggccggt gagccgatgt cctgataggt cagatagcga aacacttcgc
    21421 cgaatccatc cagccgtttt tccagcatcg catccaccgc ctcaaacgtc gagtcccgcc
    21481 gggagatccc tgttccacca ttgatcacga tggcctgcac ggactcattg gtgatggctt
    21541 cggcgatccg cgccttgatc tctgccggtt cgtctttgac gaggtggtag gcggcgatgt
    21601 ggtggccctt atccttgagc aactgctgga tcaattggcc gctggtgtcg gtctcaggcg
    21661 tgcgggtgtc gctgcaggta atgaccatgc atccgataga gcggggtgcg tggtgtttat
    21721 gttcgtgggc tgtggggcta ctcacatcga tatccgctga aggttaaaaa ggccgccagc
    21781 atcgaactcg tttcgctggg cggccttttt gattcatctc cgcgctggcg ttcgcgcgct
    21841 acttccagac cgaatccggg ttcagcttcg ctgccggttt gccgcccttg atgtgctcat
    21901 cgagaatggt agcgacatcg gcttcagtga ctttggaata ccaggtgttg tccgggtaca
    21961 ccacgacggt cggtccctgc tcgcaggggc cgaggcagga ggagccggtg acgaggactt
    22021 cgccaggcat gatgccgcgc tgcatgagac ccatgttgaa ggacatcagc agttgcgcag
    22081 agccctggcc gccgcaggag ggtttcgggt ggcccggcgg acgggcgttc gtgcagacaa
    22141 gaatatgata tttcggtttt ggcatcgtga cctcaatgta tggggtgagc gacgactgcg
    22201 tgagttcgtc aggctggttt atacgctttc cactgctcgg tgcattcctc aggcaacttc
    22261 cactgcttga tgcccttgag tacccaatcg atgtaatgct ctttcggctt gaatttgccg
    22321 atgggattgg ccgcatgggt ggtgaccaat aacttctcgc cttcttccgt gatcacggtc
    22381 accggcagat ggcggtaggc gccctgcggc acatcgcctt cgaagtcgtc gaggatcttg
    22441 agatcttcgt cggtgatttc gaagacggct ccccagactt tttccccagg tgacggaatc
    22501 acgctcgcca ggccgcatcg ccattgcgtc gaccagcggc agaactgaat gctatggtcg
    22561 gggaggtacg ccgtgaaaag aaacttgtgt tccggggcgc gacgcttgag ttgagacagg
    22621 ttcaaattgt ccgcgtaaaa gaagaacttc attgaacagt gagactccga ttatgtcgcg
    22681 aaggttgacc ggtacttgta ccatagcgtt gcgcgacctt gtcaagcgca ggggctcctg
    22741 cgcgtcgccg gtttcaacgc atcccgctgt tgttcgacga ttgacagtga attcacacgc
    22801 cacctatgat gacgagttga tcaaccatcc accgcagttt attgtgacga cctatgagta
    22861 aagtgatcca ttacacggtg cttgcgcttc tcctggccgg agggttggcc tattggtgga
    22921 tgaaaccacc cggcctcaac ccgatgctcg actcgcaggc ggccgaggcc ctcgcactgg
    22981 tgcaaaccca tcgggccgcc ggttatccga cgattcttca ggcgttcaac gagcgtgccc
    23041 gcattcagga agcccgcaag ttgggcttgc gcttgggaga gtggaaagtg atcaagcagg
    23101 acggtcagcg ctatgaggtc cggatctaca tgcgcgagca ggggacgacc cagtggttcg
    23161 aacgggattt catctggcac gtggatctgg cgacgaagcg cgtcaacgcg gcctcgttgc
    23221 cggccgacgg gttgatgccg gaaggcccgg agagtggacg accgcgcgca ccgggcggcg
    23281 aagtccctcc gttcccgcct acaatgtaag gggcgttttc acctgcacgt ttgtgccttg
    23341 caccctcact tccacgcaag ccacgcggat ctccggattt tcaggccgct caccggtacg
    23401 gacgttgaat cgccacccgt gccaggggca ttcgaccagt tcaccatcca tgcatccttc
    23461 gccgagtggt ccccctgcgt gcgggcatgt attatcgacg acatgaaagg ttccgtcgac
    23521 attacagagg gcgagaaaaa ttccctcgac ttcaacggtg cggcaggtgc caggaggaac
    23581 ctggttcacc tgggcgacgg tgatatagtc gggcttcgaa tccataagcg tcctctgcaa
    23641 gttacagcgg ggtacaggtc gtctcaatat tgcgtgacat cgtgagcagt attgtctaag
    23701 cgcttgattt cattggccgc atatggcata gattgagcag gcatttttca gggaagggtc
    23761 acaacagcgc cgtcatttgc tatcctgtaa atagtgcact cataggagat cgccggagcc
    23821 actatggaaa tgaccttgtt gctcaactcc acctatgaac cgctccgtgt cctgcattgg
    23881 caaaaggcca ttactctgct ctggcagggg aaggtcgaag tcctcgaagt ctacgatcgc
    23941 caaatccacg gtatctcgat ctcgatcaag ctcccctcgg tgatgcgcct cctgaagctg
    24001 gtgaagctca aggacagcca tcgcgcggtg aagttttcgc gtatcaacat ttttacgcgc
    24061 gacggctact gttgccagta ctgcaagcac aagttccgca ccgaggaact gacgttcgac
    24121 catgtcgtgc cgatcgcgaa aggtggtcgg aagacctggg agaacatcgt gacggcctgc
    24181 tggcggtgca ataaccggaa gagcggccgg accccggaag aagccaacat gcgtttgatc
    24241 aagaggcccg taaagccgcg ctggagtccc accgtcacga ttaccatcgg gatccggaac
    24301 acgccggaaa gctggcggga ctatctgtat tggaacctcg agctggacgc ggatccggct
    24361 gaaacctagt cggctgttga aacgggttct tggcatcgca ctcgcggtgc tcaccacttc
    24421 aaggtacaag ccaagtgcgc ccggcctctc gctggttgca gctttaccga atcacctgtt
    24481 cagacagcct gccgcagcta atacaccttg tcgatggtaa tgttcacgtc tttcccgccc
    24541 ggtaacgtcg gattctttgg gtagcgtccc tccatgtcac ccggttgcgc ctggccggcc
    24601 tggccatctc gatccagacg cgcaatcaca tccaccatgc ctttcaattc ggtgccctgg
    24661 accatgacat cggcactcgt aatctcaaac tgcaccggaa atttcggcgc gtcgatccgt
    24721 ttggccgcga tgggtctcgg tggtccggtc ggacgccgca cgatgacgaa cagcacatcg
    24781 gtcgatttga cctggtcggc caggttgggc gcgatctgca cttgcccggc aatcgatccg
    24841 ccgccttgat ccggctcccc gatgtgggcg atgccgaaat aggccgcgac ggccgcaatg
    24901 ccgatgatcc ctccggccat cgccagggat cgtcctgtat atccagccac aatagactcc
    24961 tttgttgact cgcggtgtgt gcgacgggct cgcccgtcat ccagcgggcg cattataccc
    25021 agctcctgcc agaaagggga atggcggagc gggtttaccc tgtcaaatcg gctgttgtat
    25081 gatacgcgtt cacgtcgttg tcgatttgtt ggaggatgaa ctcaatggct gcaaatcccg
    25141 ggtttcaaat ttcactcgat gtgcgtgggt ggccggtcct ggtgatcggt ggtgatgagg
    25201 aagcggcaga taaagtgcag cggctcctag aggcaggcgc gaaggtcacg gtgatcagtc
    25261 ccaccctgca tgaagtgctt cgtaaactgg cggcctcggc gaaaatcatt catcgtgggc
    25321 ggcatttccg cgcgaacgat ctggaggcgg tcatcctggt cttgaacacg attcgcgatg
    25381 atcgtgaact ctcccactcg ctcatgcgat tggctcgcga acagaaattt ttgatctggt
    25441 ccgtcgacca gcccgacgtg tcgaacgtgg tgatgccggc cgtcgtcagc tccggtcatg
    25501 ttcgtgtggc gatcagctcg agcgggcagg cgccggcgct ttccggattc atgaaggaag
    25561 atctcgaacg tattttggac aacgagtttg tcgcgttcgt cgattggctc gggcagcttc
    25621 gcgagcaggc caaggccaac gaaccggacg cggagaaacg tcgcgcgctg ttgcgtgagg
    25681 cgctggatgg attccgactt ctgggaaaag tgcagtaccc gaaggtgtgg ctggatcaac
    25741 gggcggccgt tggggcaggg gaaaagcgcg gatcgtagga ccggtcgttc gttatcggcc
    25801 gacagttatc agcaggagat tcccactatg gtgttgctgg aattcagtat gtcgcccctg
    25861 ggtaagggag agagcgtgag caaatacgtg tcacgctcgt tggatattat cgacaagagc
    25921 ggtgtggact atcggctgaa tccgatgggc acggtactcg aaggggaatg ggacgaggtc
    25981 atggcggtgg tcaagaaatg ctacatgcgc atgcgcaaag actgcaatcg cgtgtcctgc
    26041 agcatcaaga tcgactaccg caagggaccg aaggggcgct tgtcgagcaa agtgtcgagt
    26101 gtggaatcac agctgaagcg aaagctgaag gtctaacagg ctgcggaaaa actcgattgt
    26161 tgcgcagtac gctacaatca aattatgtgc cgtgtcggta tcgccataac ccgcaggatg
    26221 tgcaaaaagg ccgtccaggc cgcagcgagc gaagaggcga atcgtactcg tgcggtacgg
    26281 tgagccgcga agcgacgcga gaacgccgct ggcggacttt tttcgcatcc tgctaaggtg
    26341 cgcgttacgg ggctccgatg tccgcatggg cgtcgtagtg aagtgttccc tcggttttga
    26401 atcgcaccga gaggttggcg gtctgcaccg tcagcgcttc gagggccagc ttgtgaaccg
    26461 tgccgcgcaa gcgccctccc gtcaggtttc tgttcagtcc cttctccagc gcctgtcgca
    26521 tcaggtccag ttcctctcga atggggaaga ccaatcttcc ggccaactca tcacgcagcg
    26581 gttcatgtaa ccattcttca gccaggtcgg tggccggcgt gcgctccttg atcgtgtagt
    26641 cgacatcccg aaacagaatc cgccccgccg cttcgtcgta cgccggtgtt ccctttaaac
    26701 ggagtgtgag cggcagcagc cctcgcaggg cgagttccac tcccacttga ttgcccaagg
    26761 ggtacaatgt cgctcccacg atcttgatcg tgccgacgcc cagcgaccat tcctgcccga
    26821 ccaccgcttc acgcagccgt tgattggcct cctcgatggg gacctggagc gcgaaggtga
    26881 cgtgaaagcc gtcgtcgtga aactgagact gcaggtcggg gagggggaga tgtcgtgata
    26941 agggcttgat gccgcgcacg acggccggtc tggctgtgac gccatacccc gcggccggtg
    27001 ttcctgagag cggttcgact ccggcggggc cgatggcttc aggattcagt tggagccaga
    27061 ggccttccgc ttcatccaac agcaagggtt cctgcagatc gttccagatg tgagtgacgg
    27121 cgggcttcag cgttacgatg gtggcagcct tacgatcaac cgtcggcagc atcccttgca
    27181 actcacttcg atagacctgc gccatcaggg gtgccgtatc gacgccttgt tcggacagca
    27241 ggcagggtcg gttcgcttcc accgcggtaa cgctactgac gggcaatagg gtataggcgc
    27301 gggcctggct gaaggtggtt gccagggtca cggacaatcg acctgtgccg gttgtggcat
    27361 cctgccggcc gcattcggcg attttcgtca ccgtgccttt gatctgcttc gcgtattcca
    27421 cgccgaatgg aaagctggat tgggacagca ggcgacctcc ggtcgtcgtc gaactctgct
    27481 ctcccggcca gatccggtac ttcaaaaatc cggtacctcc gcccagcgtt acccagtcgc
    27541 ggtgtgtgtg tcgatgtgcg gcgagtgact ggtgaatgct cgcatactgc tcggccaggg
    27601 cggtcatggg aatcggcaca tgaatcgtca tgctggaggt ttccatctgc tggtgccggg
    27661 ccgtgtcggt tcctgccgct gccgcgggtg ctgtggcgca gagactcaga atggccgcag
    27721 agaagcaaac caggctattt cgatcgaacc ggcgtcgtcc gcccatcgtc ctatcctcat
    27781 gctggtggca cagagggtgg ccgccccccg aacttacttt gaacatatcg gtcgactatg
    27841 ggggaagcaa aaacggaagg caagccgtca cttcttcggt gaggacgttt tgtcggctga
    27901 tagcagcggg agatagtggc gaggcacacg ctggccgatg gcgcagagca cttcataggg
    27961 aatggtctgc tgccaggcgg cgagatcggc tgcggtgatg cgctcttgtc cctgctgtcc
    28021 gatgaggatg gtttcatggc cgacttgcac gccgggcacg tctgtcacat cgaccatcgt
    28081 catgtccatg cagacacgcc cgaccaccgg cacccttcgt ccgccgatca agaccacgcc
    28141 tcggttggac aggaggcgat tgtagccgtc cgcatagccc accgggagga ccgcgatccg
    28201 tgatcgccgc gaggcgacga aggtccggtt gtagctgacg ctgtcgcccg gctggatgtt
    28261 gtgcaggtgt gcgatggtcg ctttccaggt gaggatcggt ttcaattcag gcgcggcggc
    28321 tgcgttaggc agggtatggt agccatacag catgattccg ggtcgcacga gggaatagag
    28381 gcttgcggga tacttgatga tcccggcgct gttggcggcg tgtgtcaggg gacaggagat
    28441 cccacgttgg cgcaacgtgt cgagtgtctg ctgaaactcc gcgagttgtt tctccgtgtg
    28501 tgtggtctcg gtattgtccg catcagccag gtgagtcatg aggccttcga gccgtagcgt
    28561 ggcgcgaaag gccggcatgg cgacgaggtc gggcacctct tgagggagga tgcccaatcg
    28621 acccatgccg gtctcgattt tgacatgcac ggagtagggc gcggcattgg gcgaaatggt
    28681 tgcggcaaaa acttggacca tatcggaccg gtacagcacg ggcgtcagac gatgtgcgat
    28741 caggtctgga aattgtgccg gtaccgtcgg gcccatgacc aaaatggcat catgaatgcc
    28801 cgcctggcga agcgcgatgc cctcatccac cgtagcaaca ccgaaccgat ggacggccag
    28861 ttgttgcagc gtgcgtgtga gttcgagggc gccatggcca taggcattcg ccttgacgat
    28921 agccagtacc tcggaacgcg gcgcaagacg acgcacgtga gcgagattct gcgcgagggc
    28981 attcagatcg actgagacgg aggttggggg gaactgagac ggcgtgtaca cgcagcttgt
    29041 ggcggcagaa tagggcgcta gacttccaga atctcgcctt cttttttctt catcacgtcg
    29101 tcgattttct ggacgtattg atcggtgagt ttttgaattt cggcttccga cttgcgcagt
    29161 tcatcttcgg tcagcttcgt gtctttctga agcttcttga gttcttcgtt gccatcccgg
    29221 cggaagccgc gaatctgcac cttcacttct tcgccgtgtt ttttgcagac cttaataagt
    29281 tctttccggc gctcttcagt gagcggtgga agtgggatgc ggatgagttt gccatcattg
    29341 gagggcgtca agccgaggtc cgaggtttgg atcgccttct cgatttctct gatgaggttc
    29401 tgttcccagg gctggatggt aatgagccgg gcttcggggg tggcgacgtt ggcaacctgc
    29461 ttcagcggtg tcatggttcc gtagtagtcg actttgatgc cgtccaccag tgccacggat
    29521 gcgcgaccgg tacgcagtcc ggccaggtcg cgtttcaaat gttcgatcgc gtggtccatc
    29581 ttttcggtta cgcgttgttt cacttcttgc gccgtcgaca tcagcctctc ccttagcgac
    29641 tttctacggt gaccagggtc ccgataggtt cgccctggac gacacgttta aagtttccct
    29701 tctctttcaa attgaagacg atcaatggca accggttatc catgcacaag ctgatggccg
    29761 tggagtccat caccttaagg ttctgggtga gaatcgacag gaacggaatt tctgcgtact
    29821 tcttggcttg tggatttttg accggatcgc tgtcgtagat accgtcgacc ttggtgccct
    29881 tcatgatgac ctgggcgccg atttccatcg cccgcagtga tgccgcggta tcggtggaga
    29941 aatagggatt tcccgttcct cccgcgaaga tcacgacacg ttttttttcc agatgtcgga
    30001 tggcacggcg gcgaatgtag ccttcggcca gctgccgcat ctcgatcgcc gactggacac
    30061 gggtactgac gcctttacct tccagtgcat tctggagggc gagtgcattc aacaccgtgg
    30121 cgagcatgcc catgtagtcg gcggaggcac gctccatgcc gcgcgcactg gcggccaggc
    30181 cgcggaagat attgccgccg ccgatgacaa cggccacttc gacgtccatc gcgacgacgt
    30241 cggcaatttc ttctgccagc ccttcgagaa tcgagggttg aacgccatag ccctgctcac
    30301 cggccagcat ttctccgctg actttcagca ggatgcggcg gtagtgggca gaactcatgc
    30361 ttggcctagt tggaatcgtg tgaaccggcg gacattcatg ttctcgccga ttttggcgat
    30421 ttgttgcgcc agcagatcct tgatgacgac ggacggatcc ttgataaagg cctgttcgag
    30481 caagcaattt tcctggtaga acttctcgag cttgccctcg atgattttcg gccaggcggc
    30541 cggtggcttg ccgagttcct tggcttgccc ttcgtagatg ctgcgctcct tcgcgacgac
    30601 atcttcggca atctcttccc gtcggacgta ggacgggctg gcggccgcca cctgcagcgc
    30661 cagatcattc acaaacgctt tgaactgctc attccgcgcc acgaagtcgg tttcacaatt
    30721 gacttcgatg agcacaccga tctttccacc ggcatggata taggcatgga tcaagccctg
    30781 gttggtctca cgaccggcct ttttctgggc cgccgctaag cccttctgcc ggagataatc
    30841 gatcgctttg tcgatgctgt ttccgttctc agtcagggcc ttctggcaat ccagaatgcc
    30901 ggcccctgtt ttttcacgta actctttgac gagctcactc aaacttgcca tgggttcctc
    30961 agttggtcga atagaacaat gccatcacac gagacgcggc cgtcaggagg cggccacgcc
    31021 ggccaaattt gccgacggtt tagcgccacc accggctggt gccgatgcaa actctgtttc
    31081 ttctcgctgg gtacggaggt gcgctccttc aaggcacgcg tccgcgatcc gggaaataat
    31141 cagtttgatg gaacggatgg cgtcatcgtt gcccgggatg gggtattgaa tgtggtcggg
    31201 atcgcagttc gaatcgacga tggcaatgac cggaatttcc agccggctgg cctcgaggat
    31261 cgcaatgtgc tcgatgcggg tatccagaat aaacaccgcg ccgggcaggg cgcgcatgtt
    31321 gcggatgccc gacaggttct tctgcagctt gacgacttcc ttctgcatct gtccgagctc
    31381 tttcttcgta tggccctgat gcgtcggatc agcgagggtc gcttccattt ttttcatttt
    31441 gtcgatgcta cggcggatgg tctggaagtt ggtcagcatg ccgcccaacc agcgctggtt
    31501 gatgtagaac tggttggccc gcttggcctc ttcctctaaa atctccgccg cttggcgctt
    31561 ggtaccgacg aacaagacgg agtcaccggc cgccacggtg tcgcgcgcga aggcataggc
    31621 ttgttccatg cgttcgacgg tctgttgaag gtcgataatg tagacgccgt ttcgttcccc
    31681 gaacaggaat cgcttcatct tggggttcca acgattcgtc tggtgtccga agtggacgcc
    31741 cgcttccagt aattccttaa tcgcaactac tcccatacct tcacctccct ccgaagcctg
    31801 cagagcgcct ggcgaacgcc aacgacgagg gggatgtccc ccttattctc aggctgcgca
    31861 gcgtgcgcat cacgctggtg tggatttaaa taagacgtcc ctgattttga atgcgaaaat
    31921 gtgaacgatg ggacggacca ttcactgcac gaaaacgccc gaacgctagc ataatgccca
    31981 gtattttgca acctcgcgag gtggctcacg atcggtaact cgcgttgatc cgcacgtatt
    32041 cgtaggacaa atcggtcgtc cacatgtgcg cctcggcttg gccctgtgcg aggtcgacac
    32101 gaatcgtaaa ctccttcgcg cggaagacct tggtcagctt ctgctcggcg acgcggccca
    32161 gtccttgacc gttccggacc atcgggacat ccccgaattg caggctgacc cggctgggat
    32221 cgatgggtac ccctgcgcgt ccgagggccg ccatcacacg cccccagttg gcatcaccgc
    32281 caaagagcgc ggtcttcacc aggttggagg tggcgatcgt ttgggcgatt tgtttggctg
    32341 ccatcgtgga tcgagcgcgt gtgacttcca cccgaacgac cttagtcacg ccttcgccgt
    32401 cccagcaaat ttttaaggca agggtgcggc agactgtttc gaccatggcg cagaaccgtc
    32461 gaaagtccgc agagcctggc ttgagtgtct gattgcctgc cattccgttc gcaaggcaaa
    32521 ggacggtgtc gttcgtgctc gtatcgccgt cgacggtaat acagttaaac gattgatcca
    32581 ctgcctgact cagtgctgtc tgaagcgccg accggccgat gaccgcatcg gttgtgagat
    32641 agcccagcat ggtggccata ttgggatgga tcatcccgga acccttggcc attcctccca
    32701 ccgtcaccat ccgccctcca aggcgggcgc gcaccgccac ctgtttgggt ttgaggtccg
    32761 tggtcatgat ggcttgggct gcttcgtttc cgccgcggcg cgtgacgcga gcgaggagag
    32821 acgggacggc gttcttgatg cagtcgatcg gaagtggttg accgatcacg ccggtcgatc
    32881 cgatgaagat agtgtggggc gcactgccga gggccgtggc aaccagctgc gccatctcat
    32941 tggccactcg cattccgtcg ggtccggtgc aggcgttggc gttcccgctg ttgacgagga
    33001 tggcccggcc gatgcccttc ttcagatgca ggcgatcgag cacaactgga gcagccacga
    33061 cctgattggt ggtgaacacg ccggcgacgg gtccctcctg ctcggagacc agtagggcca
    33121 agtcgagttg tttcttctgc tttttcttga tgccgcaata catcccggcg gcccgaaagc
    33181 cgatcggtgc agtgatgcct ccagtgatga ttttcatagt gatcctgtgt ggtgagaaat
    33241 ggtccgtagc gcggagtcgc tcaggactca aattatggat agatacccgg cgccaccagg
    33301 cccgtctctt ccggatagcc gagcatgaga ttcatagcct gaatcgcttg tcccgctgct
    33361 cccttgatca ggttgtcgat ggccgacacg gtcacgaccc agccggctcg cgtatcggtg
    33421 tggactgcga gatcgcagta gttggcgcct cgcacatgtc ggggattcgg catcgcgcct
    33481 tcgagcagtc gcacgaatcg ttctcccttg tagaagtccc gatagagcgc ccgcagttcg
    33541 acgacatcga gcttaccctt catgcgcgca taggcggtgc tcaagatgcc tcgattcatc
    33601 ggaaccaggt gcggcgtgaa tgcaatggtg acagccgaag cgtgtccgtt gccggcctgt
    33661 ccacgcgttc ctgccagccc gctcagttcc tgttcgattt ccgggatgtg tcgatgctgg
    33721 ccgatcttat agggctccag tgattcgtgt gcttcgggga agtggtaggg cagggcagga
    33781 cttcttcctg cgcccgacac gccagatttt gcatcaatca caatggtgtc cggcacgatg
    33841 aggccatggg cgatcaacgg cgccagttgc agaatcgcgg cggtcgggta gcagccgggg
    33901 gaggcgacaa gtcgggcctg tgcgatggcg gatcgatgca gttccggcag gccatagacg
    33961 gcgcttccga tcaggtcggg atgcgcgtgt gtggtctgat accaggtttc gtatgtgcga
    34021 ggatctttga gtctgaagtc tgcgctcagg tcgatcacgc gcttgccggc tttcatgcaa
    34081 ctcgctaccg ggcccatgga ttttgtatgg ggaagggcga ggaagagtac atcggcgcgt
    34141 tctgccaggg cctcgggcgc cagggcttcg aaggtcagcg gcacgatgcc ctgcaaatgg
    34201 ggatacacgg tggagacggg ggttcctgct gacttctcgg aagtcaccgc agtcaattga
    34261 acgttggggt gttgcgacag cagccgtagc aactccgctc cggtgtatcc gctgccccct
    34321 gccactgcta ctcgtaccga cgtcatgtca cccctcccgg cctatcgcga attgttgctc
    34381 gatcagttca gcgcacacat aaaaaaaagg gaaggctgcg gagccttccc ttttgacacc
    34441 ttgtcgctcc agtgtttctt tatcgtttcg agtattggaa gcgctttctg gcgcccttct
    34501 ggccgtactt tttgcgctct ttcacgcggg agtcacgggt cagaagacct tccttcttga
    34561 gcgggcttcg gaatgcagcg ctcatgacga ccagcgcctt cgagatggca tgccgcaagg
    34621 ctccagcctg gcccgcaggc ccaccaccat agacggtggc ccggacggaa tacttaccgg
    34681 ccacgccggc tagttcaaac ggaaattgaa tgacattccg caaggtcatg cgaggaaacg
    34741 ctttttccag cggcttctcg ttgacgacga tatcaccagc agcggcggtc acccatgcac
    34801 gagcgatcgc gctctttctc ttcccagttg cgtactgcgt aattgctgcc atatcaggcg
    34861 gcctccttca cagatcgaat ccgaacaata atgatcacag cgaaagtgat tccagttgct
    34921 gcgcgtgatg tggatgggtt gaacctgcgt acacgcgcag tttcttggcc atctgattcc
    34981 caaggggatt cttgggcaac atgccttcga tggcgcgcgt caagagcttc gtcgggtcct
    35041 tcttgtggat atgttcggcg gtgagggtct tgagtccgcc tggatatccc gtgtggtacg
    35101 aataggtctt gtcttccatc ttgttgccgg tcaaccggat cttctccgca ttgacgatga
    35161 cgacatggtc acccgtatcc acgtgaggag taaaagtcgg tttatgcttg cctcgaagca
    35221 aggtggccac ctttgctgca aggcgaccga gtgtcttgcc gtcggcatcc accaggaacc
    35281 atttccgttt cacatcagct ggcttctcaa gatacgtttt tgtcatggtc tccatctact
    35341 cctgcacgaa aggattctaa tgtgaggtat caggcctcac gcctgttggg gttcgatatt
    35401 cttagtgtcg agtttattga tccaagcatc caggtgaacg gctgtccgcc ggttaagcac
    35461 atcttgtgtg gcataaatcg ggcaatctgc gcgaagtgcg agggcgatgg cgtcgcttgg
    35521 cctggcatcc agggtgcgat gcacgccttt cgatgcaaag gccacgctgg catagtacgt
    35581 actgcctttc acatccgtaa tcacaacccg ctcaatccgc acaccaagat gatccgcaaa
    35641 gctccgaatg aggtcatggc tcataggacg gggagtgacc acccgttcca tggcaagacg
    35701 aatggcgtta ccctcggccg atccaaccca gataggcagt gtgacgctcg cgtcgtcatt
    35761 tttcagcacg acgatgcggg tgtcagtatt gccgtcgtcc agcacctgct tgacggtcag
    35821 agcgacgagc tccgagggct catgctgctg cttcgacgag tccatgaact aggtatccag
    35881 tttcccgaaa tcttccggct tcaaattttc aagccactgt tcaagttctt cggacgactg
    35941 tttcttaatg accttctcgc tggcgaaaat cggtgcactg gttcgcagcg ctagcgcgat
    36001 ggcatcgctg gggcgagaat cgaccgtata ttccgagtct tcgtacatca ggtgaatcgt
    36061 ggcgaaatag gtgttctcgt tcaaatctgt aatcaccacg ctgataattt tcgcatcgaa
    36121 cgtttccagg aacgacttca tgaggtcgtg ggtcatggga cgcggtgcgg tgacgctctc
    36181 cagcgcaagg ccgatggcac tggcctctga ttttccgacc cagatcggga gcatgtctga
    36241 attctcttcg tcccgcaaca ccactatata ggcgttgttg taggggtcga agatcagacc
    36301 ctttacccgc atttgtgtga tcacggcggg attatccttc ccgatgtcgg ccaaaaacct
    36361 caagaatagg gcactctagc acttccgaaa tcagagtgtc aatgaattcg atcgggaaac
    36421 ggatgaagag agggccgacg gttcatcact cttgacgcct caagctttga tcggacctca
    36481 ggcgagtgac gaacaggcag atcatgagcc gttctgcctg gcgccacggt ttgattggag
    36541 gtgatgaccc taagagccgg ctggacgaaa gttctgatcc agtttggacg tgtcggtttg
    36601 ttctcccagt ttgaggaacg ccttcatttg ctttttaacg agttcgcggc cgctctcgtc
    36661 gccgagattg acttgatact cgttgatcac catgacccgc atcccttccc attttttcca
    36721 gcagggcttg caaaccttgg ccttgatttc ggcttcgagc tttccgagga acagcatatc
    36781 cgtaatcgct tcgcctgctt gtccgcaggt gacacattgc acatctgcca tgattgcctc
    36841 cgtaagtctg cctgatcttc acttgtccgt cgcgcggcat tctagcattc gcctgcagca
    36901 gaaaaaagag tgtgccgtgc caattgacgc ctactcgggt tcatgttaaa aatgcgtcct
    36961 ttcaaagagc cgagtggcaa ccaaggaatt ggcgtcgcat gacacacgtg cccggatggc
    37021 ggaactggca gacgcgccgg actcaaaatc cggttctcgc aagagagtgg gagttcgacc
    37081 ctccctctgg gcaccatcat ttggacaccc cttccatggt ctcgacaacc agtgcgctca
    37141 tgcagacatt gccgtctggt gttggtgcga tgcgaataaa tagacgatgc ttttcgacac
    37201 aacactatat agtggatgtc gtggccgaaa acatcttgac atgatgtggg tgttagccta
    37261 tagttcgccc gaatttgctt ggaactacta tggcctgttc agtttatcgc cgttcatcat
    37321 tactctctta tcaattccaa ctcactaagg aggcagcaca tgcgtaacgt gttggcactt
    37381 ggagctgcgg tgcttttcat cagctccgtt ggtgttgcag gcgctcaaga gcgtttgcca
    37441 cagtcctcgt cgggatcagg tttcggtgtt ggcaccggag tcggagacgt ctcgggtaac
    37501 tccggacgga ccgcagcatt tgaccggacc gcattcatgg accggcttgc tcaggcagaa
    37561 accgtgtttg gtcgcgtcct cgccgttgat gttcctggcc aaaaattgca cctggaaaca
    37621 ggtggaagct cgcacgacga aggtcgtgct ggtacgggcg cgatgtcctc gttgacgctc
    37681 tatattgacg ggcaaaccaa catggaccag ataagagtgt tgaacgccgg tgacgatatc
    37741 tccgtccagg tcgtagaatc cccaaatgtg agagcagatc agggggtcta cggaaccgga
    37801 cgcaagctcg tccgtgaggt gtatttgttc cgcggtaacg agaaattggc tggttttggc
    37861 ggccttggac agcgtccgaa tccggcaacc gagcgtggac ttgagacgaa ctcgggtact
    37921 gtggtcggcg ggctggtcgg tggtgtgttt cctggaacgg tgaccaccgg tgtggtcacg
    37981 accactgtcg gtgaatttac cggacaggca ccctgctgga actgcgaacc gcagccagga
    38041 tggggctatc agacggtcgc tccggaaacc aaagtgcaga acaagtctga ctacggaacc
    38101 gacggtgcca agccaaactt gcagaaaggc ttgacgaact aagattcatg ccacgacagt
    38161 ggtaggtgtt gagcggggtg cttcggaaga ggcaccccgc ttttcttttg gtcacacgat
    38221 tcctcgcagc ccgttgcaac gtaacagttc gattgatacc atctgctatc acttctatcg
    38281 aggtcttcta tgtctgatgt cccattgaat ctccaggcac ctgacgaaga gctggatctc
    38341 cgcggtgtca tttgccctta caatttcgtg aagacgaaac tcaagcttga gagcatggcc
    38401 ccgggtcaac tgttgctggt ccatcttgac gacggagatc ccattcgaaa tgtccctcgc
    38461 agtgtgagcg atgacgggca tgatgtggta tcgcaggaac gtgccgatca gtcctaccgc
    38521 gtcatgatcc gtaagcgctt cgacgagtag ttatcgcgat tgccttttgc tgatcttcct
    38581 tttcgacttg gcaccttcta ccactacagc gacgtgtgac gcctgttcgg ggccaggctt
    38641 gcgcgagagc agttgctgaa ctcgtcctcg taggatcttc tcagtactcc gggtcatatc
    38701 gatagccagc acgatcttcc ggttaccgag catctgttgc agtagagtca aaaactgcgg
    38761 gaggtcccgt gccggaggga agaaaatcat cgtgcgtggc tcggattgta ataattgaag
    38821 acggcccgtg agtgctttag tgccaccggt ccaacgcccg tcaaagacga aggcattgcc
    38881 gtccattccg gcgagggctg ttgccgccac cacagcggaa gttcccggga tgaccttgac
    38941 gggaatctgc gccctcgatg cggctgtgat gagcaatcga cccggatcat agacaaccgg
    39001 cattccgcaa tccgacacca gcgccactcg acatccctgt cgcaagcgtt ccaacaatag
    39061 tggaactttg tcggcggcat tgttccgatc ataggtcgtg atcgtggtgt ggattccgtg
    39121 gtgggccagg agggcgtgcg tggcctgcgg atttttagcg gcaatgagat caacctgccg
    39181 aagagtcgcg agacctcgga tcgtcagatc gtcaggatgt ccgatcggca cgccgacgat
    39241 gaataacgtt cctgccgggg gggcagattg cgcgcgcttt ccttgactcc gttctcgtcc
    39301 catcgatata atttccagtt accagaaaaa gtggctttgc gctgaaatgt tgcgaggata
    39361 gagcacgatg tcttcggtgt ttttcatgct cacgatgggc ctgtatttcg tgagcacggt
    39421 ttgctatctg gcctatctgc tccggcgggc ggaaaccctg tcaaaggtgt ccctcggaat
    39481 cacggctgtc gggtttgcca cccacacggt ggccttggtc gcccggatga cggacacggc
    39541 ccaggcgcag ctaccgacat ttcacgaagc cctgtcgttt ttctcctgga tgctgattct
    39601 cgtgtttctc gccgtcgaat ttcgccgcca gttgcatgtc cttggttcct tcatcgtacc
    39661 actggcgctc gtgtcgttgg tcaccgctgc ggcgttccgc tccgacgagg cgtccagtct
    39721 tcagccggtc ttcaaaacgt tgtgggttca cgtcaccctc agcatgttgg gcacggtcgg
    39781 ctttgccgtc gcattcgtcg ccggggtgat gtatctcatt caggatggtc ttctcaagtc
    39841 gaagcgcttc aacgtgctct acgcaaaact gcccgcgctg gattatctcg atcacttgaa
    39901 tcagcagtca attgtgatgg ggtttccgct cctgacgctc gggatcatca gcggcgcctt
    39961 ctcggcggag atcgtccgtg gcacctatgt gaactggaac ccggaacaga cgtgggccat
    40021 ggtgacctgg ggcttctact ttgccgtcct ggtcggacgt ttgaccatcg gatggcgtgc
    40081 caaacgagcc gcgtatctga cgatcatcgg gttcgccggc gtgattctga ccctgatcgg
    40141 tgtggtgttg aaaagtcacg ggtcggtgtc ctgacatgca tgtcatcgtt gttgggctca
    40201 gccataaaac tgccccggtc gagattcgcg agaaactcgc cgtgcctgaa agccggctca
    40261 gggaagccct cagtcgtctc tgtgcttatc ccggtgtgcg cgaagggctg ttactttcga
    40321 cgtgcaaccg ggtggaagtc tacgcggtcg tcgaagagtt ggaaaccggg tatggtcgcg
    40381 tgcaggaatt tctggccgac acccatctct ccctttcctc cgaacatctg acgcctcacc
    40441 tctattggca tgccggcgat cgcgccatcg gtcatctgtt ccgtgtggcc tccagcctcg
    40501 attcgatgat tgtcggtgaa tctcagatcc tcgggcaaat caaggatgcg ttcgaagttg
    40561 cgttgacgca taaagcctcc ggcctgttgc tgaataagat cgtcaagaag gccatttcgg
    40621 tggccaagcg cgtgcgcacc gaaaccaaga ttgccgagac agccgtgtcg gtgagttatg
    40681 cggcggtgga gttagccaag aagatctttt caaacctgac cgagaagacc gtgttgctga
    40741 tcggggccgg ggaaatggcc aagctggcgg cgcggcatct gatcgccaac ggcgtgcgcc
    40801 aggtgcgcat taccacccgt aatttccagc acgggttgga attggctcag cggttcaacg
    40861 gtacagccgt gccgtttgag gactacaaga cggaactggc cggggccgac atcgtgctgg
    40921 tgtccaccgg ggcctcgcat tacctggtga cggccgacga tgtgcagcgc gcaatgagac
    40981 agcggatgag tcgtcccatg ttcttgattg atatttccgt gccgcgcaat atcgacccgg
    41041 ctgtccggcc catcgatgat gcgttcctgt tcgatatcga cgacctgcac atgcgagtgg
    41101 agcaaaaccg tgaagaccgt ctgcgggaag cggcgaaggc cgagcaaatg gtcgttgaag
    41161 aagtcgcggt gctcgggcag tggctgcagt cactggaggt gacgccgacg atcgtggcac
    41221 ttcgcagccg gaccgaagaa ctcaaacggc gggaagtcga taagattctg gcgcggttgg
    41281 cgcatctgtc gtccgaggat cgagccgcgg tggaagcctt ggcgtccgcc atcgtcaata
    41341 agatggttca tggcacgatg gtgacgttga aagctgaggc caagtcctcg agcggcgcgg
    41401 cgtatgttga tgcggctcgc cggtttttca atctggaaga gacacccgct gtgtatccgc
    41461 ccggacaagc cgacgtctcg gagcttcggg gcgagaacat cgccgggaac ggagttggtc
    41521 gcctgcccga ggcgtcgctg gtccagcaca cgagcaagga gcaaggccgg cgggtgagct
    41581 aacaccgccg ctgagaaatt tcatggtcag attcgtttcc acatgtgatc gggtatccgt
    41641 ggccgctcga gcgagtgacg gtcactcatg agtcgttcaa cacttatcct tggtacgcgc
    41701 ggcagcaagc tggccgtgca tcaaagccag tgggtgcagg cccgtctcca ggaactcgcg
    41761 cctggcctga ccatctcgct gcagcgcatc caaacatcgg gcgataagat cctcgacgtt
    41821 ccgctggcaa aaatcggcgg caaaggactg tttgtaaagg aaatcgaaga cgcgctgttg
    41881 agcaaagaga tcgacctggc cgtgcacagc atgaaagacg tgccgacggc tctgccggag
    41941 gggttggaca ttctgtgcgt gccgccgcga gaagatccgc gggatgcgtt gattacgcgc
    42001 gacggttgtc ggctggacca attgaagccc ggtgccagaa tcggcaccag cagtctgcgc
    42061 cggcaagccc agttgctgca ttaccgccct gatttcacca tcgagatgtt gcgcggcaat
    42121 ctggatacac gcctgaggaa actccgtgag gggcaattcg acgcgattgt cttggccgcg
    42181 gcaggattgc ggcggctcgc ctgggatgcg gaaattaccg aatatcttcc cgtgcatctt
    42241 agtttgccgg ctattgctca aggtgccctc ggcatcgaag cccgcagcga cgacaccttt
    42301 gtgcgcgaac tgttgagccg gtttgagcat cggcccaccc gcatcaccgt cacggcagaa
    42361 cgtgcgctgt tgcatcgcct cgaaggcgga tgccaagtgc ctattgcggc gcatgccgcg
    42421 cttgagggtg acaggctcac ggttgatggc ctggtagcca gcgtcgacgg ccgtcgggtg
    42481 atccgtcatc aaatccaggg tccggcgagt gaagcccagg ctctggggac gaagcttgcg
    42541 gagcgtttgt tggccgatgg gggtgatgtg atcctcaagg agatttacgg gcaggcctga
    42601 gtggtatgac gacgcgagca ggaacagtat atttggtcgg ggccggtccc ggagatccta
    42661 agttgctcac gatacgcggc aaggaatgtc tggagcaggc cgatgtggtg ctgtacgact
    42721 atctcgcaaa tgaagcgctc ttgcagcatg tgtctccaac ggctgaacgt ctgtacgtcg
    42781 gccggcgcgg gcgcggccag tatcgcgatc aggcggagat caatcggttg ctgatcgatc
    42841 acgctcaagc ggggaagcgc gtggtccgct tgaaaggtgg cgatccgttc gtctttggtc
    42901 ggggcgggga agaggcggaa gccgttgccg ctgccgggct ggaatttgaa gttgttcctg
    42961 gtgtgacggc cgccgtcgcg gcgccggcgt acgcgggtat tccggtgacc catcgcacga
    43021 tggcgtctac cgtgaccttc gtcaccggac acgaagaccc cacgaaagat cgaagtgggg
    43081 tcgaatggcg gcgcttggcg accgtacacg gcacgctggt gtttctcatg ggcatgacca
    43141 acctgccgaa aatcgtccaa tgccttcggg ctgaaggtaa agagggcacc acacccgtcg
    43201 ccctcatccg gtgggggacg cgagcctcgc aacgaaccgt tgtgggaagc ttggacgata
    43261 ttgtggagaa ggcggcggcc gctcatatgg aaccgcccac ggtcatcgtg gtcggcgagg
    43321 tggtgcgtct tcgggagcaa ctcaattggt tcgagcgccg gccgctgttc ggaaaacgga
    43381 ttctagtgac gaggccgaag cctcaagcgg tggagttttc cgatttgatc gcggcccaag
    43441 gtggagaacc ggtggaatgt ccgaccatcg agatcgtgcc ccccgaggac tgggctccgc
    43501 tggatcgggc tctggctcgt ctgtcgacct acaattggtt gatcttcacc agtgtgaacg
    43561 gggtcgctcc attcatgacc agactgttgg agacgcaacg tgacgttcgt gcattgtccg
    43621 gcctcaccat ttgttgcatc gggccgcgca ccgctgaggc tctggccgcc tatggcttgc
    43681 gtgccgacgt gattccggaa cagtttcaag ccgagggaat cctggaggtg ttggccgccc
    43741 ggcagatgcg cggggctcgc gtgttgattc cgcgtgcatt ggtggcccgg gagctgcttc
    43801 cggatcagct acgtgcccag ggggcggacg tggatgtggt gccggtgtat cgcacgatca
    43861 ggccagcagt cgcgacggat cgcctcatgg aacagcttcg taccggaggg attgacgtga
    43921 tttctttcac gagttcctcg acggtgagga acttcgtcga gttgtttacg acgaaggacg
    43981 agttgttgcg ctcggtcggg aaagcgaccg tggcctgcat cggacccatt actgcggcga
    44041 ccgctcgcga ggcggggctc accgttcagg tgatggccgg acagaacacc gttccagcgc
    44101 ttgccgacgc catcgtggat tttgttgtga atccgattga tggccgaacc gcttccgcgg
    44161 tgatccattg accggatggc agtgatcatg aagatgtttc ttaccaagag ggaggatgcg
    44221 gcatggttcc agttaagtca ttcatggttc cgaaggacaa gttcatcacc gttgaacgtg
    44281 acatggatgt gcgctctgca gggcgggtca tgcgcgatcg caacatcggc agtctgttcg
    44341 tcacgaacgg aaaagatgtc atcggcattc tgacagacac ggacatggta cggcgggtgg
    44401 tcgccaccgg ggccgacatg accaagacga ccgttgagca gatcatgtcc gcccccctcg
    44461 tgacgatcga agaacataag acgctgctgg acgcgaacga cttgatggcg caaacccatc
    44521 ttcgccacct cggcgtgacg aaggacgggc agctggtcgg catgatttcg gtgcgcgatc
    44581 tggtggtgtt cctgaccaac ttgccgagga agtgagcgga ccatggcatt tccgattcag
    44641 cgcctgcgtc gtctgcggga gacggagccg cttcggcgga tggttcgaga aacgagcctg
    44701 acgccgaacg atttcatcta tcccgtgttc gtcaccgaag ggcagggacg tcgtgaaccg
    44761 atcgtctcga tgccgggaca atcgcgattg tcgatcgatc tgctggtcaa ggaagcgcat
    44821 gaggtcaagg ccttgggtat tccggccatg atcctcttcg ggattcccga tcgaaaggac
    44881 gagcgcggca gttccgggtt cgaccccgag ggaatcgtgc aacgcgccat tcgtgcagtg
    44941 aaggcgcagg tgccggatct ggtgctgatc accgatgtct gcatcgatga gtacacgagt
    45001 cacggccatt gcggcatcgt gcgggacggc aaaattctca acgacgagac gctggattgt
    45061 ctgaggacca tggcgaagac ccatgcgcag gcgggggctg acatggtcgc gccgtccgac
    45121 atgatggacg gccgcgtggc cgcgattcgc gatgagttgg attgcgccgg atttgtcgat
    45181 atacccatca tggcctatgc ggccaagttc gcgtcctgtt tttacgcacc gtttcgtgat
    45241 gccgccaatt ccagtccgca attcggcgat cgccagtcct atcagatgga tccggcgaac
    45301 aagcgggaag ccttgcggga gattaacctg gatatcgagg agggggccga catcatcatg
    45361 gtgaagcccg ccatgccgta tttggacatc atcagcgccg cgcgcgaacg gacgcttctg
    45421 ccgattgccg cctaccaggt gagcggggaa tacagcatga tcaaagcggc ggcacaagcc
    45481 ggctggttgg atgagcgccg cgcgatgctg gagtcgctgc tctcgatcaa gcgagcgggc
    45541 gccgagatca tcttgaccta ttttgccaaa gaggccgcca ggcttctcca gtcacggccc
    45601 gcatgaagat ttcccgcggt ctcaccccgt ccacgtccag accctattcg gttgtgacca
    45661 tcggaaattt tgacggtcac catcatggtc accgggctct cctcgaccgg gtggtggcga
    45721 cagcccgtcg cgaggccggg acggcgctcg tcctgacatt cgatccccat cccgtcaaga
    45781 tcctggcccc gcaggtcaat ctcatgtttc tgacgacacc ggaagagaag ttggcccggt
    45841 tcgaggccgc cggtatcgat gaggtcgtct ttcttgaatt cacgacggcc tttgccgcgc
    45901 tgagcccggc tcagtttgcg aagcaggtct tgtgcgacgg gatcggtacg cgggagttgt
    45961 atgtcgggga acattttgcc ttcggaaagg gccgtgccgg acgcattgcg gatctgctcg
    46021 agctcggcgc ccagctcggg tttcgcgttc atccgatggc gcctgtgacc attgacggcg
    46081 aggtcgtgag ctctacgcgt attcgtcagc tgatccaggc gggggagctg cagaaggccg
    46141 ttcgcctgct gggacgtccc tacagcatcg aagggaccgt gatttcaggc gcccatcgcg
    46201 gaacggaact cggttggccg acggcgaatc tccgcctccc tgaggggcgt gtcattccgc
    46261 cggatggcgt gtatgcggca caggtctcct ggaaggggag tggcttgaat gcggtggtct
    46321 acatcgggac caggccgacc ttcggcgccg gcgagcgact cctggaagtc tcgatcctcg
    46381 atgagcgact cgaattgtat ggagaagtga ttcgagtaga cctgctcggt ttcattcgtg
    46441 aggatcgcgt ctttgcctca gcggaagccc tgacccggca aattgcgttg gatgtggatg
    46501 cggcccgtgc acagttgcgc gatgcaggga tcaccgcgtc gctctcggcc ctccagcccc
    46561 ggtcatgagc catgacaggt tcccagtcag atctccatca cccgcttcat aagcaggtcg
    46621 cccgggctat tcgtgcgcgg aggctcctgc aaccggggca gaaggtcctg gtggccgtgt
    46681 ccggggggcc ggactcggtg gcactgctct ccattctgca ccagctcgca ccggcctgga
    46741 atttttcgct gaccgtcgtc cattgcaact acgggttgcg gggcgccgaa tccgacgggg
    46801 atgcctcgtt tgtcgcggca ctctgccgtc gcctcgaact tccgtgtcat atcagacggc
    46861 tcacggtaga ccgcagtgga acaggggagt cgagctcatt gcaggcgcga gcccgggagt
    46921 ctcgctatcg gctgtttcgt gacgtggtcg ccgaactcgg cgcagatcgt gtggcgctcg
    46981 ggcatacggc ggatgaccag gccgaaaccg tgctcttgcg gatgttgcgc ggggccggtt
    47041 tgcgaggatt ggccggcatg ccgcatatcc gggagagcct attcgttcgg ccgctcctca
    47101 gtcgttcgcg ccaggagatc ctggcctatc tccaggccgt gggattgtcc tatcggaccg
    47161 actccagcaa cgccaaatcg atctatcttc gcaatcgtgt ccggcacgaa ttgctcccgg
    47221 tgatgcagtc gttagccccg gctgcagccc gcatcctggc gcgccaggct gatctcctac
    47281 ggggcgatga tcggctgctg gatgcgctgg ccgcacaccg tctcgagcgg accattcgga
    47341 gccgcgattc ggctacgatc gtgctcgatc gtacagtgtt gctcaaacag ccgccggccc
    47401 tacagcgccg gattctccgt caggcgatcc aggcggtggc gccgtcaacc ggcgcgccgc
    47461 gaagcgacgt cctgctgtcg atgttggcat cactggcctc acattcggga ggcatctgga
    47521 acgcgggtgc tgcgacggtg gcctgcgaac aggatcaggt gaggttgacg gcggtctccc
    47581 cagtgcctcc ggcgggtggg acagttcttc agccgatcca tacggctcca gtccccgatg
    47641 ttgtgacgat agccgccctc ccttcggccg tttcctggcc attgacggga gcggtgattc
    47701 gtctgcgcgc ggtgacgcgc gagcggggat tggtcctcct ggagaagcca tcgtcttccg
    47761 tggctctgct ggatgccgac cgattgtcgt ggccgctgac gattcgcccc tggtgccacg
    47821 gggattggtt ttttccgacc ggcatggcag ggcgacgaaa gaaattgcaa gattacttca
    47881 cagatgcaaa actgggtcgg tctatgcgag aacggatccc actggtgttg tcgggtgagg
    47941 gcattgcctg gatcgtcggg cagcgggtcg atgcccggtt tgccgccacc ggttcgacca
    48001 tccgatttgt cctggcccgt gtcacgcagc ctctacgccg gaaaggagtc ttctaggaat
    48061 ggaacgcatt tttggtcgcc ccattgtgac gcaagagcag atgcggactc gcatccgtga
    48121 actcggccgg caaattgcgt ctgattatgc cggaaaagat ctcgtgctgg tcggggtgct
    48181 caagggcgca tatgccttct atgccgatct ggcgcgggcc atccggattc cgatgcgggt
    48241 ggagtttatc gtggtgacca gttatggcgc cggcaagaaa agttccggca aggtcaagct
    48301 ggtgtcggac ctgacggagc ccattgcggg caaagacgtg ttgctggtgg aggatatcgt
    48361 cgattccggc ctgacggtgc aatatctcat gaagacatta tcccgtcgaa agccccgatc
    48421 gttgaaggtc tgcacgctgc tgagcaagcc cgatcgtcga accgtcgagg tggatctcga
    48481 gtacgtggga ttcaagatcc cgaataaatt tgtggtcggg tacggcctcg actatcggca
    48541 ggagtatcgc aacctgccgt accttgcggc gctcgatcag ggggatgaac aggagcagga
    48601 gtctgcatga aaggttgttc cccctgcgga acgtatggta acattctgca gggttagcta
    48661 atttttcggt ctgcctaata tcctcaaagg agaggtggat gaattcacgt gtaaagaatt
    48721 tgctgttctg ggtcgtcgtc ggcctcttca tgattttgct gttcaacctg ttcagcgtgc
    48781 cgactcacgc gccggaagat gaagtcatat tcagcgattt catggccaag ctcgacaagg
    48841 gcgaggtcat gaaggtgacg atcaaggcca accacatcag cgcaatttta aaggaccaaa
    48901 gccggatccg gacctacacg gccgagtatc ctgaattggt caagcacttg cgtgagaagg
    48961 atgtccagat cgaagcccgg ccccctgacg agagcccctg gtacatcacc ttcctggtga
    49021 cgtggggacc ctttatcctt ttcctcggtc tctggttctt cctcatgcgc cagatgcaga
    49081 tcgggggaaa taaagcactc tcgttcggaa agagtcgcgc gcgcatgctg accgaagagc
    49141 gcaaaaaggt gacgttctcc gatgtcgccg gtattgaaga agcgaaggaa gaagtccttg
    49201 agatcattga gttcttaaag gatcccagaa aatttcaaaa attgggcggg cgcattccga
    49261 aaggtgtcct gattgtcggt cctccgggaa ctggaaagac gttattggcg aaggccattg
    49321 ccggcgaggc aggcgttccg ttcttcagta tcagcggatc ggactttgtg gaaatgtttg
    49381 tcggagtcgg tgcttccaga gtccgagatc tcttcgagca ggggaagaaa catgctccct
    49441 gcatcatctt catcgacgaa atcgatgcgg tcggtcgttt gcgtggcgcc ggtctcggtg
    49501 gcggccacga tgaacgcgag cagacgctca accagctgct ggtcgagatg gatgggttcg
    49561 acacgacgga aggcgtcatt ctgattgccg ccacgaaccg tcctgacgtg cttgatccgg
    49621 cgttgctgcg cccgggccgt ttcgaccgcc aggtcgtggt caaccggccg gacttgcgag
    49681 gccgctcgga gattctgaaa gtccacacca agaaggtgcc gctggctgcc gacgtggaat
    49741 tggagaagat tgcgcggggc acgcccggat tctccggcgc cgacctggaa aatctggtca
    49801 acgaagcggc gctgtgggcg gcgcgctgga acaagaaaga agtcgagctg atcgacttcg
    49861 aaatggcgaa agacaaggtg ctgatggggg ccgagcgcaa gagcatggtg ctcagcgacg
    49921 aagagaagcg cactacggcc tatcatgaag ccggtcacgc actgatggcc aagttgctgc
    49981 cggggacgga tcccgttcac aaggtcacga tcatcccccg tgggcgggcg ctcggtgtga
    50041 cgatgcagct tcccaccgac gatcgccata attactcgaa ggattttctg tacaacaatc
    50101 tcgcgattct catgggcggt cgtgtggctg aagaattggt gctacacgat gtgacgaccg
    50161 gggcagggaa tgacttggag cgggccacag acctcgccag aaaaatggtg tgtgaatggg
    50221 gcatgagtga aaagttagga cctctgacgt tcggcaggaa agaagaggaa gtattcctcg
    50281 gccgtgagat ggggtcgaag cgtgatttta gcgaacaggt tgcgctcgaa atcgatcacg
    50341 aagtcagacg tctggttacg gaaaattatg aacgtgcgaa gcgcattttg accgataata
    50401 tgacgagcct gaaggcgctt gctgaggcgt tgttggaaaa agaagtgctg gacgcgttgg
    50461 agatcgatca gatcctcatt caaggatcct cgtcgcagac ggttcccgcc taagctctgt
    50521 atcgctgttg tcgcaggggc ttcattcgtg ttcccccctg cgacaacagc gcttcctata
    50581 caattcgtgc cacgggtcgg gaagccgctc ctatacatga ccgccgtcgg tttgatttcc
    50641 ctcccgcagg tgcgttatgt cgcagactga tgtcctgttc ctgatccgga agagagaagg
    50701 acctgcctga ttgtgaccac gacctcatcc tcaagccctc tcctcttaac tgctggctct
    50761 cggacgttca gttttgaatc aggtccactc ctcatggggg tgctcaacgt cacccccgat
    50821 tcattttcag acggcggtgc ctatctcacg gtcgagcatg ccctgcagca cgccagacag
    50881 atgcaggccg aaggtgccga tatcatcgac atcggcgctg agtcttcaag gccaggggct
    50941 caaccgatcg acgaaacaga ggaattacgt cgcctccttc ctgtcctgga agcggtccat
    51001 gacgcagtgc cgcttcctat ttccgttgat acgactaaag ccgcggtggc ccgtcgagcg
    51061 attcgtgccg gtgccaccat cgtgaatgac attagcgcat tgcgcggtga ccccttgatg
    51121 ggctcgctgg tggctgaaac cggcgcggcc gtggtgttga tgcacatgca ggggactccg
    51181 caatctatgc agcaggggcc acggtacggc catgttgtgg aagaggtgct tgcattcctg
    51241 cgcgaacggg tgcaggtcgc gatcgctcac ggcatcaagg gaagtcagat cgtccttgac
    51301 cccggcttcg gttttggtaa gctccaagag cataatcttc aattgctcgc tgagtttgac
    51361 gccttcacga agctcggtta tccggttctg gcaggggtct cccgtaaaca gtttatcggg
    51421 aacctgactc aacagccggt gcaggaacgg gggtacggca ccgccggcgc ggtggcagta
    51481 gccgtcctta aaggggcccg gatcattcgt gtccacgacg tgcgatcgat gcgagatacc
    51541 gtatccgtcg tgtcagccat ttcctgtcac gcgcgttctc atgtaggggt atccaatgcg
    51601 taaattgttc ggcacagacg gtgttcgcgg ggtggccaac ctcgaaccga tgaccagtga
    51661 aatcgccatg caattggggc gagcggccgc ccatattttc atgcggcgcg caggccgaca
    51721 ccaggtggtc atcggaaagg acacgcggtt gtcgggatac atgttggaat cggcgctgac
    51781 gtccgggatc tgttccatgg gagtcgacgt acttctagtc gggcctttgc ccactccggc
    51841 gattgccttc ctcacgcgaa gtctgcgggc ggacgccgga gtcgtaattt ccgcgtcgca
    51901 taatccctat caggacaacg gcatcaagtt tttttccaac gaggggttca agctgcctga
    51961 tgagcttgag gcgcgcatcg aacagttgat cgtttccgac gagatcaaac atcttcgccc
    52021 gacggcggat gcgataggaa aggcctatcg tatcgatgat gccgaaggcc ggtatattga
    52081 attcgtcaaa cgctcgcttc ccagagatct cgattttcag gggatcaagc tggtggtgga
    52141 ctgtgccaac ggtgccgcgt ataaggtcgc tccagccgtt ttccgcgaac tcggcgcaga
    52201 aattgaagtg atcgcaaata ctccggatgg aatgaacatc aatgacggct gcggagccgt
    52261 gcatccggag cgcctgcagg aggccgtgcg tcggcatggc gcccatatcg gtattgcgct
    52321 ggacggggac gccgaccggg ccatttttgt ctgtgaacag ggaaaaatca ttgatggcga
    52381 ccatgtcatg gccgcgctgg gactcgattc gcacgcgcag ggccaacttg cccgcgcgac
    52441 cgtggtggga accgtcatga gtaatttcgg gctggaaatt gccatgaaga aggccggaat
    52501 ccagctgatg cgcacgcccg tgggggaccg gtatctcatg gagcggatgc tggccgacgg
    52561 atacaacttc ggaggagaac aatccggcca ctttattttc ctggaccaca acaccacggg
    52621 cgatggcttg atctcggcgt tgcagattct ctcgctgatg aaacgtaccg ggaagccgct
    52681 gtctgaattg gcgaaggcga tgactgccgt cccgcagatt ctgttgaacg tgaaagtgaa
    52741 gcataagccg gatctcaacc aaatcccgga tatccagcag gccattaagt cagccgaagt
    52801 gaccctgaac gggagcggcc gtgtgcttgt gcggtattcc ggcaccgaat cgctgctccg
    52861 catcatggtc gaaggcgaac gtgattccac catccgtgag gtcgccgatc atcttgcgga
    52921 gatcgtgcgt gcccgcatcg gatagcatcg ggccgatgcc gtaggcgccg gatgttaccg
    52981 actcttacga tcatctttgt gctcggcctc gttctcggtt cctacctcac gtattttcca
    53041 ctcagcatcg cgatcgtcct gctggcttgt actgccggct gcgtgtggct ggagcgtcag
    53101 catcgcctca cgtcacaaca gagcaacgtg ctcctggcct gtctgttcgg cggatgtctg
    53161 tattggaccc tgtgtgcatc gttcactccg cacgtgctgc tgcaggatag tgcccgtgtg
    53221 cttcctgtcc ggttgaccgg aacgattgtg gaggccgttc gccatgcgcc gggccgtgtc
    53281 acggctatgg tgcaggtgac gggcagcgac gacccccagt tgccgatacc gtttcacttg
    53341 cgtcttacgt ggcgagatcc ggaccgcgat ctccaccgcg ggttgcagat tcgtactcgc
    53401 atccatgtcc atgcaccctc gggaactctg aatccgagag gattcgacta tgcggcctat
    53461 ctggatgcgt tgggtgtcga tgccgtcgga tcggtctcag gagccggcgt catcgaggtg
    53521 cttgatccag accgggaggc gccgcttcac ctgttcgcct ccctcatcga agaatggcgc
    53581 ggccgggtca gagccgccgc cgagacgctt ccccagccca gtcgaggcct gtttctcagt
    53641 ctgaccatcg gtgaacaggg gtttcttgca ccggaagtcc gtgaatggtt catgacgacc
    53701 gggacggtgc acattctctc gatctccggc tcgcatctcg gtctcatcgc cttgctctcg
    53761 ttcgcattgg tcaggaaggc gtgcctgttg ctgccgtcca tacttctgct gagcctttcg
    53821 cggtggctca cggcgacgag actggccgcg ttgctgacac tggttccagt gctggcctat
    53881 acggtgctgg ccggggcgga gaccgccacg attcgtagtg ccatcatgat cgccgttgcg
    53941 ctctggaccg tatggctcgg ctcaccgcac tatctccttc acgcactcgc tgccgccgcc
    54001 ggtctgacgc tgttggtcca tcctgcggcg ctgttcgata tttcctttca actgtcttac
    54061 gtctcggtct tggcgctggc cctggcgctg tcgcgcgaga cgcctgtcga tgaattgccg
    54121 cttccgaagg aatcccctgc cggacggatg gtgtactggt ttcgcgagtc gctgcgactg
    54181 acggcgttgg tgacgcttgc caccgtgccc ctggtggcct gttacttcaa tcaagtgtca
    54241 tggatgggtt tattcgcgaa tcttctcatg gtgcccttcg tcggtttcat cttcttgccg
    54301 accagtttgt tgtcggccgt ctgggtgatt gtgacgcaca gcagcacgct ccctggcgct
    54361 gcgttcatcg attcgttggg gcaagggctg attgcttcga cacatgtgct ggccggtttg
    54421 ccgggcgcgg aatggttcgt ggccgcaccg acgattccca tgatgctgct gttctatgtg
    54481 ctggggtggg cgctgttggc gggatggccg gcctatgctg cgccgctgct cagaaggtcc
    54541 atggcctcgg gccttgcttg tatcctgctc tggtggcttt ggtctccacg tcccttcggt
    54601 ggtgaggaga tggtgcgcgt gaccttcctc gacgtggggc agggtgatag tgcggtcatt
    54661 gaattgcccg atgggacggt cacgctgatc gatggaggcg cgacctatga gcggtttgac
    54721 atggggcgga gtgtggtggc gccttttctg tggagtcgag ggattcgacg catcgatcat
    54781 gtcatcggga cacacccgca attggatcac gtcggagggc tggcgtggat cctggctcat
    54841 tttcaggtag agaaattctg gaccaacggg gcggagcggc aggaagaatt ctggcgcagg
    54901 atcgagcgag cgatggtgca gcggaagctc gaagccacgg tggcgacgga aggccgattg
    54961 atttccgaag ggcctgcctg ccgcatggtg gtgctcaatc ctcagcccag gccggagtcg
    55021 tcgatctccg caaagagcga gtcgctgaac aacctgtcgg tcgtcactga attggtctgt
    55081 cgcgagcagc gtgtcctgtt tacgggagat atcgaacggg aagccttgac ccgtctcacc
    55141 cattccggga attccgctca cattgccctc ttgaaggttc cgcatcatgg ggcgaagagt
    55201 tcgctggagc gaaggtggct tgaaacgatc aggccggccg tcgctgtggt gtctgccggt
    55261 cggcacaatc cctatggtca tccggccgga gaagtcttgg cggcctacca ggcggtggaa
    55321 acgcaggtct ggaggacgga ccgggatggt gccatctggg ccgatctcga tctcactcga
    55381 caggaactct ccatgcacag caccagagaa tggatactgc aaccggcgct gccatcggcg
    55441 gacatatggt ccgtggagca ggacaatctg cgccgcttat ggcgccgttg gaattggacg
    55501 tagccccagc ctgctcgttg ccggcccacc gttcatgctc tccgtgccgt caccgaacat
    55561 cttcccatcg ctcaacgatc tccctccggt gcagatttag ccgtctcgga tggcagcagg
    55621 ctgtcaattt tgccgacagg caggatacct ataccgcgta agtcgtcaat attgtgtaag
    55681 agccccccag aacatcggca gggcatagca catgcagacg tgctgcactg tccggtcatg
    55741 ctgtctatca caggccacag caggtcgaat ggcatccgta ttcttgagtc ggataccgga
    55801 agcagcaaag actcgactcg atgggctgaa gcagtcaggc gatacagcca aaagcatggt
    55861 ccgtgcggtc cccggccggc ggtacatcag actgggggat tgatccggat gattctggga
    55921 aaaaaagcag caagatcatg acgaagcgtc tcttgtttct ggctcctgcg ccgccctccg
    55981 accggcaggg tgggggagcc ttgcgcatgt tgcacctgtt gcggttcctg gggagtcgct
    56041 ttcaggtgga tctgattgct ccggcgctcg acggcgcgga ggatgcgcaa cgacagttga
    56101 aggatgtctg cgcggagacg atgtttgttc cgcagcaagg gcccggtttc ctcgatcgaa
    56161 tcggccaggt gagtccctat gtcaaagatc gggcgttggc tgccgtcgtc cgtgaacgcc
    56221 tggcgagcgg ccaatacggg gccgtgcact tagaaaagcc cgcgatgatt ccctatctcc
    56281 cgccccatgt gtcggtgccg gtggtgctgg atgtctggtc ctacagctcg acgagtccgt
    56341 tgcggatact caggggcgta ggcggagcta ccggtcggtc gagacaactg gtccggctca
    56401 tcaaggtggg gatcttcgac cggttccgct ggcccatgac acattgtgtg acggtcgtgt
    56461 cggacgagga tcgaatccgt tgcgaacgtg cccacccggg ccaacgggtg ctggtggttc
    56521 ccaacggcgt cgattgccgg acgatcctgc ccaagccgga tcatgccgct acgtcgcctc
    56581 tgttgctgtt cacaggtgat atgggggcgg aaccgaatgt cgaagccgcg ctcttcctgg
    56641 ccacggaagt gtttcctgcg attcgacggg attttcccaa ggcggaattg cgattggtcg
    56701 ggaggaaccc ggacccccgc gtgcgccgct tggcaggctc cggcatcgag gtgaccggcg
    56761 cggttcccga catgcaactt catctgcgag ccgcgacgat gtatgtcgcg cctctttgta
    56821 ccggcaccgg gtcccggaca aaaatactgg aggccatggc ggcgggcctt ccgatcatca
    56881 ccacgtcagt cgggatcgaa gggatgaagg tacaaccggg gagggatttg ttggtggccg
    56941 atcagcccgt cgacatgatt gagtcgatcc acaccttgct gatgagccaa cccgatcgcg
    57001 agcggttcgg ccatgcggcg cgccatatgg cggagatctg gtatgactgg agccgctgtc
    57061 tctggcccct ggagccgctc tatcagaatc ttctcattcc gaaggccgtg gcctgttgag
    57121 cgctgccacc gggcggctgc gaatcacatc cggtgacagc tctacgatct cgctataacc
    57181 ccagttcggc acggcacgac aggaatctgt tgtgttgtgc cggagcctcc acgtaaaatt
    57241 tcctgccggc acgatttcag gctccgactg ctccctgctg gctcggcacg acaagcggct
    57301 cctgacgcga tgacgatgat tccgcattca aaaccttccc tgggacaaga cgacattcgt
    57361 gcggcgaccg aggtgctccg atccggtctg atcgccgggg gacccgtcgt cgggcaattc
    57421 gagcgtggta tggccgcgta tctcggggtg cagggcgggg tggcggtcaa ctcaggcacg
    57481 gtggccctgg agttggcatt gcgtgccatg ggagtcgggc atggcgacaa tgtcatcctg
    57541 cccagttatg tctgtccggc gccctggttg gcggtccagc gagtcggtgc acaagccagg
    57601 atcgtcgata tcgatccggc tacctacaat ctggatccgt acaaagtccg caaggcccgc
    57661 acgtcacgca ctcgggcggt gatcgtgccg cacctgttcg ggttaccggc tgatctgact
    57721 gtgttggaat ccctcgggat tccactgatc gaagattgtg cgcagaccct cggtgctatg
    57781 gaacagggcc gcgcagtcgg cagcgtcggt ctgctcacgg tctgctcgtt ctacgccacg
    57841 aaactgctct gcaccggcga gggcggcatg gtgctgtcga acgatgaggc gttgctggag
    57901 cgggtgcggg cacttcgcga gtacgatcaa gcgccgtcac tcaatgcggc ggcgttcaac
    57961 tgcaagatga cggatctgca agccgcgatc ggcgtcacgc agctcaacca gctgggagaa
    58021 tttctcgaga agcgagcagc gttggctgcg gtctatcgcg acagcctgcc tgccgaactc
    58081 ttcactccgc cctcggttcc ggcaggacga acccatgtct actatcgatt tgtcgtgcgg
    58141 ttgcagaaag ggctgcagtc gtccgatgag ttcacggcct atctctcgcg tatggcgcat
    58201 cgcggtctgc agtgccgcaa gcccgtcttc cggcccttgc atcggtatct cgagttggct
    58261 gattttcctg ccagtgacga ggccgatgat gtcgccattt cgctgccgat ttatccatcg
    58321 ttgagtgacg atgatgtgaa gctggcatcg cagatcctgc aggaagagct ccggtaacgc
    58381 tgccttcgta ttgatcgctc tctcgaccga cccgcgtggt cccgcaccgc gtatggttgg
    58441 gcaccctgcc atcataccct tgacccgcgc cctgctgatc gtcatgatgg cacgattttt
    58501 cggtcgtcct gagatgacgg caaattcctt cccccttacg caaccggagg acatgcctat
    58561 ggtgcccacc gtcgagtgga aagacggagc ggtccgtctg ctggatcaaa gccgcctccc
    58621 gatgcacgtc gaattcctcg actgtcgcga ttatcgcgcg gtggcgcagg ctatccggga
    58681 cttgaaagtc cgtggcgcgc cggcgatcgg tgtcacggcc gccatgggtg tggcgctggg
    58741 ggcgcaggca gtgacggcca ccgagtaccg cgactttgcc acgcaggtgg gggagatctg
    58801 cgaccatctg gcggcatcgc gcccgacggc cgtcaatctg ttttgggcca tcggccggat
    58861 gaagcagaag ctggcggcgc tcaataatca accggttgca gggatcaagg ccgagctcat
    58921 cacagaatcg cagacgatcc tggatgaaga tatcgccctc tgcaaggcca tggggcagca
    58981 tggggccgcg ttgatccaga gtggtcagac gattctgacg cactgcaacg ccggcgccct
    59041 tgcgaccgcc gggtatggaa cggccctggg tgtcattcgg gcggcgtggg agcaggggaa
    59101 gcagatccag gtcattgccg atgaaaccag gccggtatta caaggtgcgc ggctgacggc
    59161 atgggagttg atgcaggatc acattcctgt cacgctcatc acggacaaca tggccggtgc
    59221 gctcatgcgc caggggaaga ttcagctctg cgtggtcgga gcggatcgga tcgccgccaa
    59281 cggcgacgtg gccaataaga tcggcacgta ttccgtggcc gtcctcgcgc gggcgcacaa
    59341 cattcccttc tatgtggcag cgccgtactc caccatcgat ctcaagacgc aatcgggcgc
    59401 cgagattccc atcgaagagc gcaatccgct ggaagtcact gccattcacg gcagccaccc
    59461 gatcgctccg cccggcgtgg cggtgctcaa ccctgccttc gacgtcaccc cgtccgggtt
    59521 gattaccggt atcatcaccg agcggggcgt gtttaagccg ggagaactgg ccgcgcattt
    59581 ccgatcgtaa gaggcgggct gcgcctgtat cttgcatcgc tgaacgtggt gggtggtcgg
    59641 atctgcgaat ggttatccgg catttctagt agtcctttac cttgccagcc cttcagccgc
    59701 ttctttataa tgccgcgaga cggtgtctga cgcctcaatg aatgtcgatt ttttagctga
    59761 aggagcctga cgatccatga gtgaacgaac gcttgccatt gtgaaaccgg atgccgtgaa
    59821 aaagaacgcg atcggcgaca ttatcaatcg gtatgaaaaa gcgggattgc ggcctgtggc
    59881 gatgcggctg atgcatatgt cgaaagccac ggcgcaggga ttttatgccg tgcacaaggc
    59941 gcgtccgttc ttcgacagcc tctgtacgtt catgtcgtcc ggcccctgtg tggtgctggt
    60001 gctgcaaggc gacaatgcca tcaagaagaa tcgcgagctg atgggcgcaa ccgatccggc
    60061 caaggccgag caggggacaa tccgtgcggc ccatggggcc aatattgaat tcaacgcggt
    60121 gcacggttcg gatgcgccgg agacggccaa gttcgagatc gggtatttct ttgccgaaat
    60181 ggagttggtc gggtaggcgc acgttgagcg cagccaagtc ttatgtgatc cctgctcgac
    60241 ggtccttgcc gggccgtcga gccgggtcgc cacttcttcc gtcgtcagca gttcgtagta
    60301 tcggtgcatc agcgcatcgc tgatcgaca
//
LOCUS       DAORRQ010000022        54550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_32_length_54550_cov_304.366786, whole genome shotgun sequence.
ACCESSION   DAORRQ010000022 DAORRQ010000000
VERSION     DAORRQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 54550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54550
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32_length_54550_cov_304.366786"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="PK224_09005"
     CDS             <1..1556
                     /locus_tag="PK224_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRB15917.1"
                     /translation="TIQELFKTLGLTQEQPGGCLSSSTWSRTTDAGIVESRNPSTGNP
                     LARVYGCSLADYDALVTGSQQVQASWRMVPAPKRGEVIRLIGQALRVHKDALGTLVSL
                     EVGKIKAEGDGEVQEMIDMADFAVGLSRMLYGQTMHSERARHRMYEQWHPLGVVGVIT
                     AFNFPVAVWAWNAFIAAVTGSTVLWKPSPKAPLCALAVQRLCNRVLEEAGYPGVFALF
                     TTDRVELAERMVRDERLPLISFTGSVPVGRHVAEVVGHRLGRSLLELSGNNAVIVDET
                     ADLDLATRAIVFGAVGTAGQRCTSTRRLIVHESQYELLVPRLLSAYAQVKIGDPLEPD
                     MLMGPLIDGEAVTRYLSALEEVKKAGGEVLCGGCVLSRPGYFVEPTIVRAQNHWDIVQ
                     RETFAPILYVMTYRTLDEAIRMHNAVPQGLSSALFTTHLRNSETFLSAIGSDCGIANV
                     NIGTSGAEIGGAFGGEKTTGGGREAGSDAWKAYMRRQTNTINWGTELPLAQGVTFGRA
                     EGGNDGARS"
     gene            1537..2139
                     /locus_tag="PK224_09010"
     CDS             1537..2139
                     /locus_tag="PK224_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15918.1"
                     /translation="MMGQEADLDAVMARMTADFLAQNSAGRMLRRALDDVGVGFIPVI
                     DHVTIRTMDIDRRAEEFLALGYRYDETLHFDDWYAKVFRWAGYPALFVDQAYPDDRGK
                     TSIIPRWVEKFGDQVFHHVAVRVEDIEQAILKLKAKGVVFAGEIVGARGGTLRQIFTA
                     PEMLDGQPFSVLELAERHQGYQGFLPPQADSLMRSTMRGL"
     gene            2310..3380
                     /locus_tag="PK224_09015"
     CDS             2310..3380
                     /locus_tag="PK224_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRB15919.1"
                     /translation="MVQSNATLRLILALLVLCALGAGSGSVQASPSGHAPHGTVTIDG
                     VTVPDVGPLPTVVPIPSSNLNYTAKIELGKQLYFDGRLSKNNAISCAFCHNPGTGFAD
                     PRQTSIGVGGGVGGRQSPTVYNTGLNHVQFWDGRARSLEEQAIGPIHNPVEMAETHEH
                     VVAKLGKIKGYQQQFRAVFGTDVNLQGIAEAIAAYERTVLSTNSAFDKYVLGAQKAMD
                     EAAVRGLALFKGKARCILCHNGPNFTDNQFHNLGVPQVGPMKEDLGRFAVSRAEKDRG
                     AFKTPTLRSITETAPYMHDGAFKTLEEVVEFMDQGGGSNPNLSPLVKPLNLTAEEKSD
                     LVAFLKALAGEPIPFSMPQLPK"
     gene            3494..4000
                     /locus_tag="PK224_09020"
     CDS             3494..4000
                     /locus_tag="PK224_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HRB15920.1"
                     /translation="MFRNLCVLGASTIALALGGPALAADMKPGPADGFDIHVMAPHKM
                     EDGSVAGPFHHYCKAVKPEVLQCLLFESTDPNAVLTDVEYFVAKPVSRSAVPLDVWNK
                     FYHDHEVEIATGRVQVLDMPDAQAKEVAAAAAKTDGIIFHLWPKGAKAPDGSVEHPTS
                     VGHKPRTE"
     gene            4053..4607
                     /locus_tag="PK224_09025"
     CDS             4053..4607
                     /locus_tag="PK224_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HRB15921.1"
                     /translation="MRSAILMFGLCAAALTAAGCAETAREMKSSSAAPAAATAAKAMP
                     TPAQGYTIHVMAPHKFEDGSVHGPYHHYCKPISPEVLQCLLFESTDANALLTDIEYFV
                     AKSVSRAHVPLETWNKYYHDHEVEIATGRVQILDMPEAQAKEVAAIAAKTDGIIFHLW
                     PDGAKAPNGQVGHPQSVGHKHRTE"
     gene            4689..5135
                     /locus_tag="PK224_09030"
     CDS             4689..5135
                     /locus_tag="PK224_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB15922.1"
                     /translation="MDAGRLCLMGWLLLGGVSVGWAANSEVLRPRVPIDQIEAARTVT
                     NPFPVTPEMLQQGKALFEGKAFCRACHGSDGKGLGMDLDYSTFKGPLPRNFTDRLWQQ
                     ARTDGELFWILKNGSPGTDMAPFIPLVLTEEEAWQVLMYVRSFGGR"
     gene            complement(5836..6210)
                     /locus_tag="PK224_09035"
     CDS             complement(5836..6210)
                     /locus_tag="PK224_09035"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB15923.1"
                     /translation="MSHYSSASLGPLDRPPSSEAILIFQRSRDRLRIVCPVMFSGALF
                     IGEGLIHNLSQTGCLVECDRRMLEGSYMAVRLLLPDTTHALIIELAAVRWIREEYFGI
                     EFLKLPTSDQARLAHFLLAHQR"
     gene            8607..8801
                     /locus_tag="PK224_09040"
     CDS             8607..8801
                     /locus_tag="PK224_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB15924.1"
                     /translation="MALVASCALQAKIEGFHFHDLRHTFSTILVQSGIDLYKVQLVAG
                     AQIARHDATVFASLSGEFTK"
     gene            complement(8899..8974)
                     /locus_tag="PK224_09045"
     tRNA            complement(8899..8974)
                     /locus_tag="PK224_09045"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8939..8941),aa:Lys,seq:ctt)
     gene            complement(9022..9098)
                     /locus_tag="PK224_09050"
     tRNA            complement(9022..9098)
                     /locus_tag="PK224_09050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9062..9064),aa:Arg,seq:ccg)
     gene            complement(9146..9775)
                     /locus_tag="PK224_09055"
     CDS             complement(9146..9775)
                     /locus_tag="PK224_09055"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRB15925.1"
                     /translation="MEATASSPHTLPLFQQAEDWFRRARASLLGQIPCGRGCCDCCTG
                     IFPITRLDALEIQRGLDELPSALTHAIVTRARTQITAVETAYPRLKSTPGLDEWADDT
                     IDEIAERFAHLPCPALEADGTCSIYSHRPITCRTMGIPGESDGIVHGACAVQTAVPIV
                     RLSATLRRDADRLAEQEALSLSILRRAQPPGDDEILLPYGFVPNGDPTP"
     gene            complement(9781..10338)
                     /locus_tag="PK224_09060"
     CDS             complement(9781..10338)
                     /locus_tag="PK224_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15926.1"
                     /translation="MAKELPILPSTAQPAEGAAPEAVIYSRVFCKNDNAPPLRLLMEF
                     LKSRGQLPILPPDMSDAMLDEWAWVQVILGYARERKPIHVFCVRDRGSYQDLFEQEQK
                     QFLEHLSSHDSLEASLAQEHVTRARFILTTRMVEADVTDEGYDFNGWILEFFQEHCNG
                     IVQIDQQGFFSPKGELIVDLSLPEE"
     gene            10635..10922
                     /locus_tag="PK224_09065"
     CDS             10635..10922
                     /locus_tag="PK224_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRB15927.1"
                     /translation="MAKSMTKSQIADHLAGKAGITKKTAVQFLDDFAALAYREAKNAF
                     VVPGIGKLVLANRKARMGRNPQTGEPIKIPAKRVVKFRVAKMAKDSILGKK"
     gene            complement(11038..11310)
                     /locus_tag="PK224_09070"
     CDS             complement(11038..11310)
                     /locus_tag="PK224_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRB15928.1"
                     /translation="MITITLMGQLQTTDGERDLACELPTPVTVRQVIQRQGRGLRHML
                     QLLREKKVLVTINKRIASEDSLVQDGDAVRFVGHDGAGGSGLAPSF"
     gene            11445..11816
                     /locus_tag="PK224_09075"
     CDS             11445..11816
                     /locus_tag="PK224_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15929.1"
                     /translation="MPDLQFALVVSALCTSGLETLNVPEELRRRVFDACWALVSTDPP
                     PTNPRERVLDLRFGTELTLDAIVATIRQLFAAAGISMLTWDHAPSDPTRPSSPAAEPL
                     IDRLQKLYPDPPPTADPSDRN"
     gene            11946..12503
                     /gene="rfbC"
                     /locus_tag="PK224_09080"
     CDS             11946..12503
                     /gene="rfbC"
                     /locus_tag="PK224_09080"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRB15930.1"
                     /translation="MQITPTPLKGLFQIEPDVFGDARGKFVEIFRESRYDAAGIDKPF
                     LQDNFSWSVRGTLRGLHYQLSRPQGKLVTVVKGTVYDVAVDIRHGSPTFGQWYGVELS
                     DSNMRQLYIPPGFAHGFCVLSEDAGFLYKCTDVYSPPDERGILWNDPALAIAWPVKTP
                     LLSAKDQAYKCLADMMTELPRYESR"
     gene            12734..13186
                     /locus_tag="PK224_09085"
     CDS             12734..13186
                     /locus_tag="PK224_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRB15931.1"
                     /translation="MKLNSQEIEARFRRHAVRVTRQRAAIYAALAETTSHPTAEDLYR
                     TVKREHPMMSRNTVYYTLGVLRQAGLVQEVNVGHEVARFDGNVTVHHHLICLGCGQIV
                     DVMDDGLNHLAIPSRQAGGFHVLGHRVEFHGYCADCQQAGLGAMERRG"
     gene            13223..13651
                     /locus_tag="PK224_09090"
     CDS             13223..13651
                     /locus_tag="PK224_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRB15932.1"
                     /translation="MGNSLKGTKSHENLKHAFAGESQANRRYLYFARRADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFSELAEWFETLAKAERSHANRFTKGLDSLKQG"
     gene            13761..15047
                     /locus_tag="PK224_09095"
     CDS             13761..15047
                     /locus_tag="PK224_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRB15933.1"
                     /translation="MNQFNLINPIDPKDLEKETLRIYDVCDGCRRCFNLCPSFNTLLD
                     RIDEYESDVAKFTAADHHRVVDECYYCKLCFNHCPYTPPHQYGIDFPLLMAVWKKRLA
                     TERGTRWRDWLLTRTDLLGRVNSAFAPLVNWGLGQGWLRGLLQTVLGVHRDRQVLHYQ
                     RETFVRWWARRSAGRKVGTGKKAALFAGCLVNYQATDVGKATVQVLEKNGVEVALPDQ
                     SCCGMPSFDIGDTAAMVAAAERTIASLKSWVDQGYDVVIPTPSCSLMVKREYVSLVAG
                     DEAKRVAEHTYDISEYLMKMKREGGLAMDFTQKPGRVAYQVPCHLRDQNIGFKSKELM
                     ECAGAKVEVIEKCSGHDGSWSAKVEFFPLSMKIAQKAVRMVEQAPADLVASDCPLAGL
                     QLDQAGAVAHAGGRSSQHPIQIVRDAYGLPRDPRAS"
     gene            15100..15690
                     /locus_tag="PK224_09100"
     CDS             15100..15690
                     /locus_tag="PK224_09100"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HRB15934.1"
                     /translation="MKALTQQDLIPHDEYERRRETYRQAIIELKRRRRIGLGEKITLV
                     FENRETLRFQIQEMIRVERIFDPQKVQEELDVYNALLPASGELSATLLIELTDPDTMK
                     QWLDLFMGLDHGQKVGLRAGGDVVYGEFEGGHSHDTKISAVHFVRFRPTPAMVTAIGD
                     QAARVALTVRHAGYEAEAEVPWVMRQEWLEDLSTVG"
     gene            15764..16516
                     /locus_tag="PK224_09105"
     CDS             15764..16516
                     /locus_tag="PK224_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRB15935.1"
                     /translation="MASVLLNKRIEFCASHRYHKPEWDAAKNRATFGPCNNDPGHGHN
                     YMLEVTVAGEVDQRTGMVVNLFDLKRVLLQVLEEFDHKHLNLDLPYFERQIPTSENVA
                     RVLWDKLQAQPDIGTLQRLSLYEDEDLCADLTAEAGPEVASVTRRYSFTAVHDGHRGH
                     TWDLFVSVHGPIDRETGMVTDIVALDHLLKERVLVPFDGRDLRMVLATPSVTGEYLAK
                     AVWDRIVSAIPAGTLQLVKLVQTRDLFYEYAG"
     gene            16779..18023
                     /locus_tag="PK224_09110"
     CDS             16779..18023
                     /locus_tag="PK224_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15936.1"
                     /translation="MMSIWEFFGQDVALFGQLHSPMLSWLGSVGLIALFLWHAGRLTA
                     AISSVQGCYTRVWPTLTSLTASRKSLQSEWLTVPSLSDVKKPANESGGQSERVDLDDL
                     HTLDKAMRREPRLEQAWLHFRKTFIVERTAWFIEPKVFATRTAAEFFPRDLLNSRLNL
                     AFYHQFPSLITGGGLLLTFLAILIGLSKLHADGSHIVGIQGLINGLAGKFLTSIVGLL
                     CANLFVLLEKSALHRLGTTQQQFVAMVDELFPRRTMEQMLENFTPGAGPSHTPAAVGA
                     VPVDLGDRLVGTLSDRLSPTVTALREAVEVLSRREPGGRFAAPDRLAEELSRVMQQTI
                     AAPIQELNQAIHLLAHSVEELKRDQKMEPGAEPRESEFESTFFDDTLPSELEEEAIPD
                     DSMSGLRWFANWRQGASMKGAA"
     gene            18025..18777
                     /locus_tag="PK224_09115"
     CDS             18025..18777
                     /locus_tag="PK224_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB15937.1"
                     /translation="MRTNFSSDSQEQSSTLTMGITDLMTSLAVIFILLFSAYVTKVSE
                     TESQAKVPVPIPAPERKVARTTTDDIKGLLRDHFQRFDLSLDADPTDPNVVRIVVPEA
                     LLNFEFGRGALSSVADRFLADAMPTYAALLCGAMRDQIDSLVIEGHTDDRGSDIYNLK
                     LSQQRSLNVMVKGLEVIQDSAPWAYRCFHEKTSASGRGRQDLVMDESRGLDRDKSRRV
                     VFKIRLRPTAQQTEMKQAMRTIEEPNFSSRLF"
     gene            complement(18774..19700)
                     /locus_tag="PK224_09120"
     CDS             complement(18774..19700)
                     /locus_tag="PK224_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB15938.1"
                     /translation="MQVRPKPPTVSQSITHETMQTFVPIACVIALLYCLPFTGAAEAK
                     DISPRPSHSVVANPSNSAPVEPSTKFIGSHRGGPMVLIPAGEFAMGSDLGQEDEQPVH
                     RVSVKAFYLDVYETTVSRYAEFLAFQKPDAPFKWNEATAGAHENKPAVGVNWYDARDY
                     CRWVGKRLPTEAEWEIAARGTEGRIYPWGSAHPTRGHANAGETRWRGYNTLSNVGRFE
                     LGKTPEGVYDLSGNLWEWVADWYDPTYYQFSVRDNPKGPSAGPLRALRGGAWNNDSKA
                     IRSSNRAGYAPDARRNDVGFRCAQDGPSPERR"
     gene            complement(19824..20156)
                     /locus_tag="PK224_09125"
     CDS             complement(19824..20156)
                     /locus_tag="PK224_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRB15939.1"
                     /translation="MTGTVQDVRDENYKEFTESVGAVVAYGLATCEPCKQYDPILEET
                     AAKFPTIKIGKAKMHVPGRCREIKKAHTFETYPTTHFFAQGKLLLTREGVVEPAELAA
                     LISDHLLK"
     gene            complement(20239..20709)
                     /locus_tag="PK224_09130"
     CDS             complement(20239..20709)
                     /locus_tag="PK224_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRB15940.1"
                     /translation="MSDVAAEIKVGDTAPDFTLKDQDQKDVKLSDFRGKSNVVLAFYP
                     LDWSPVCQGENKCLTDDFPKFQSAHAELFGISCDSFFSHKAWADSLDLKHRLLSDFNR
                     EVVKKYGLYFEPLNCGKRATVIVDKNGKVAYVKVQEIKVAREDKDILAALAKLS"
     gene            complement(20871..21626)
                     /locus_tag="PK224_09135"
     CDS             complement(20871..21626)
                     /locus_tag="PK224_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15941.1"
                     /translation="MKIRKKSPKPAPAKSPLYLVGYRGAPPSLDELKIWYDLQYGGPL
                     TCLEHEAGGRISASHGPWQAYLLTSLPQSDAAQWQPVLSWDHRQLGTISPAATAPNTI
                     ADTVLVAARLARGLTLLTQGTAFDVLCHEYLNPSDWNDRSLNVFLPRDHVTVQQSEST
                     DESSEWFHTLGLNKFGLDELEVIQPRGLPDAETIALLKSAADTVLRSGQNQKVGSSLD
                     LPALAHTIRFIKHRTAAPTGRMMAFRQITSDIL"
     gene            complement(21663..23546)
                     /gene="typA"
                     /locus_tag="PK224_09140"
     CDS             complement(21663..23546)
                     /gene="typA"
                     /locus_tag="PK224_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRB15942.1"
                     /translation="MNSSAPSSTTSGLHAPQGRRTDIRNIAIIAHVDHGKTTLVDAVL
                     RQTHVHRKIDDMGERIMDSMDQERERGITIRAKNASVTYKGVKINIVDTPGHADFGGE
                     VERTLRMVDGVLILVDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVVN
                     RTFDLFVHLGATDEQLDFPIVYTSAIKGVATLDLKQPGTEISPLLETILDKIPAPAIN
                     ADAPFQLLVLALAQDSYKGKMGIGKIQSGSIARRQNVVTLTKDGGQVPGKISDLAVFS
                     GLERTDVETAEAGEIVALSGLEEVNIGDTIADPTNPVALPRVTIDEPTVQMTFSVNNS
                     PFAGRDGKYLTSRHLRERLFKELETNVSLRVQETDSADRFLVAGRGELHLGVLIEQMR
                     REGYELQISQPEVILHREGDTVTEPFEELTIQVPSEYQGPVIEEIGKRRGELRHMKLV
                     HSEGTASEMHIEYHIPTRGIIGLKNILLAKTRGTIILHHVFSGYAPADEKALLVAPHG
                     SLVAFEAGTSTAYALFMTQERGELFIGASVEVYQGMVVGQNSRDEDLDVNVCKQKQLS
                     NMRAAGTDEALVLTPPREMSLEFAMEYIGQDELVEVTPKYLRLRKRLLNPDDRRKAKK
                     SSK"
     gene            23720..26164
                     /locus_tag="PK224_09145"
     CDS             23720..26164
                     /locus_tag="PK224_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRB15943.1"
                     /translation="MVGLSRVDPFSSQPSNFMSELDHLLDTPEKPRRRWRWWKIAVIG
                     LLLAIVLGGGGAAGILWYFSQDLPSLDPLQNYQPSLVTRVYSDDRQVIGQFFIERRFL
                     KPIQEMPKSLTQAVIATEDTRFFEHPGLDIVGILRAAWTNIRHGGRKVEGASTITQQL
                     ARSLFLSAERTFDRKVRELILAYKMELVLTKEQILEMYLNQIYFGQGAYGVAAASQTY
                     FGKELSKLTLAESAFLAGLPKSPSHYSPFKAYDRAKKRQEHVLSRMAEAGFINAADRE
                     QAVAEKLNFRRPGSEHLGPYFVEYIRQLLVAKFGEAMVYKGGLEVFTTLNADMQKAAE
                     TAVFHGLREVDKRQGWRGPLRTVDPTTLEVPAPDPSVKLAEGDIMEGVVTKIAKDHVL
                     VQIGGSTGRLAFDDMAWATKYLKGKDPTKDATPIKNLKQLLALGDVIEVGVKKLEKET
                     VHLRLEQTPLVEGALVAVDPKTGAIRAMVGGYDFARSEYNRAVLARRQPGSSFKPIIY
                     ATAMNQGMSPATVVLDAPVVYEQEEEDKTWKPENYGKRFHGIVSLREALIHSHNLATV
                     RLLDKVGIRNVIDFSRTVGIVSPLAADLSLALGSSGVGLMELVSVYGVFANQGMRVEP
                     YAVASVQDSGGRTLDQAAIQPRQVVSRETAYLITNMMEDVIQRGTGVAAKAVIDRPVA
                     GKTGTTNDFTDAWFIGSTPNLATGAWVGFDDRRPLGETESGAHAALPIWIAFMKDALK
                     QLPVVPFEIPDGVMFVKVDPTTALLTDQDEQHGTVELFTKGTEPTKSAGSKIDPTEFY
                     KLDQLQDSAPPVSVEP"
     gene            complement(26289..26501)
                     /gene="iscX"
                     /locus_tag="PK224_09150"
     CDS             complement(26289..26501)
                     /gene="iscX"
                     /locus_tag="PK224_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HRB15944.1"
                     /translation="MDLKWSDAEELAIRLVEAHPTTDPLTVRFTDMHTWIVALPEFKD
                     DPKKSNEKILESIQMAWHEEYQDSQS"
     gene            complement(26517..28343)
                     /gene="dnaK"
                     /locus_tag="PK224_09155"
     CDS             complement(26517..28343)
                     /gene="dnaK"
                     /locus_tag="PK224_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRB15945.1"
                     /translation="MARIVGIDLGTTNSLVAYMDHGTPRVISARHERAMVPSVVALTD
                     NGLIVGDPAKEHLTRNPERTVYSVKRFMGKSLADVQNELAYFPYTLTEKGGVIRIKLG
                     EKSYSPPQISAMILKELKLRAEAFLGESITKAVITVPAYFNDSQRQATKDAGMIAGLE
                     VLRIINEPTAASLAYGLQEKTQGTIAVYDLGGGTFDISILKLKNGIFEVLATNGDTHL
                     GGDDFDQLIADLFLADIRTQYGLDLSTYSDHMQAVRLEAERAKIRLSEELKTAVSLDL
                     PESKGRFTRELTRDQLEGLCTALVERTLGPCRLALKDSGLTPGDIDEVVLVGGSTRMP
                     LVRQRVQALFGKRPHCELNPDEVVALGAAVQADILSGGTTDMLLLDVTPLSLGIETMG
                     GVMSSLIRRNTTIPASAKEMFTTYVDGQTGVDIHILQGERELAKDNRSLARFRLKVPP
                     LPAGVPRIEVTFLIDANGILNVMAKDMRTGETQSIEVKPSYGLSDQEVERMIEDSFKF
                     AADDVNARKLIEARLDAGALITTTEKSLADGGHLVATGDVEAIRTALSALATAKDGSD
                     PRAIRARMADIEQAAKGLTVAMLDDSLKQGLQGKKVSDVTQS"
     gene            complement(28349..29065)
                     /gene="hscB"
                     /locus_tag="PK224_09160"
     CDS             complement(28349..29065)
                     /gene="hscB"
                     /locus_tag="PK224_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HRB15946.1"
                     /translation="MSGHIHNTDDRRELQMARSMCWHCQSEMAGEYFCDRCVKVQPVS
                     KDLDYFTCFGLPRRLAIDPQTLETKFYELSRAFHPDFYQNKSDAEQTISLGNSAMLNT
                     AYRTLRDPIQRAEYLLDLEAGSVKDIRTTPPADLFEEILELQDTLDEFRASDRASESA
                     STLRTKLHADRATLEQRQRDMEAQLQQLFSRWDALQDRGEATEQARAERGRILKEMRD
                     ILSNRTYVNNIVNDLVATIA"
     gene            complement(29080..29439)
                     /locus_tag="PK224_09165"
     CDS             complement(29080..29439)
                     /locus_tag="PK224_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRB15947.1"
                     /translation="MDATTNTDAQAPVITLSDAALKEVKRLINVQGIEEGGLRLGVKG
                     GGCSGLSYTINFDEKIGQYDQVYEFDGVKVIVDAKSAIYLQGTQLDFQKDLMGGNFKF
                     VNPNANKTCGCGESFSA"
     gene            complement(29459..29842)
                     /gene="iscU"
                     /locus_tag="PK224_09170"
     CDS             complement(29459..29842)
                     /gene="iscU"
                     /locus_tag="PK224_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRB15948.1"
                     /translation="MAYSEKVVDHFNNPRNVGSFKKEEDGVGTGIVGAPECGDVMKLQ
                     IKVENDTIVDAKFKTFGCGSAIASSSLATEWLKGKTVEEAGKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKADSK"
     gene            complement(29885..31102)
                     /locus_tag="PK224_09175"
     CDS             complement(29885..31102)
                     /locus_tag="PK224_09175"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRB15949.1"
                     /translation="MKFPIYLDNHSTTPMDPRVLESMLPYFTEKFGNAASRNHAFGWD
                     AEEGVEAARKQIAKLIHADAKEIVFTSGATESNNLALKGVVEMYHEKGDHIITSSTEH
                     RAVLDTAKTLEAKRGVKVTYLPVDKFGMVNPEDVRNAITDKTILISVMFANNEIGTIN
                     PVKAIGKIAKEKGILFHCDATQGVGKVPIDVQDMGIDLMSFSAHKIYGPKGVGALYVR
                     KKNPRVRIAAQMDGGGHERGMRSGTLPVPLIVGFGKACELCEQEMSADGARLSVMRDR
                     LHATITKALEEVYLNGHPTERLPHNLNISFAYVEGESLLMGCKEIALSSGSACTSATL
                     EPSYVLRALGVGAELAHSSIRFGLGRFTLDEEVDYAGKRIIEVVTKLREMSPLYEMAK
                     EGIDLKSVSWAAH"
     gene            complement(31099..31578)
                     /locus_tag="PK224_09180"
     CDS             complement(31099..31578)
                     /locus_tag="PK224_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRB15950.1"
                     /translation="MLKLSKKADYGLMALQHIASNQYGDVAQARVVNTKEIAEEYHIP
                     VELLAKVLQTLSKSGIIESHNGPKGGYLLGKNPREITIAHVLESLEGPLGIMDCSHEK
                     DGDACMQREHCNIRTPLLKIQSSIYQLLNNMTLQDMMGGTPLITIQSVATEQQGVER"
     gene            31785..32201
                     /locus_tag="PK224_09185"
     CDS             31785..32201
                     /locus_tag="PK224_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRB15951.1"
                     /translation="MGGTNPYIEKADVELPQKSYIVTFIFPDKTEKKVEVQPDKIPYG
                     PTGLPGSILDIAMGNGIDLEHVCGGVCACSTCHVMVKKGLETCNEGTDDEFDQLDEAP
                     VTTLQSRLGCQCVPNGTVEVVVEIPAINKNLAKEGH"
     gene            complement(32240..32938)
                     /locus_tag="PK224_09190"
     CDS             complement(32240..32938)
                     /locus_tag="PK224_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB15952.1"
                     /translation="MAEPTQIAEIISLTTLTPHTTELVLRPIEHPLSFKPGQWISLQL
                     PVGDHPPLNRAYSLAEPPSTAGHLTLLFDHVPGGKGSGYLASLKPGNRIPLSGPYGHF
                     VLPDSEPQHLLLIGRYSGLVPLRCMLRALASQGSLPSSTLIAQAPTTAEQLYHEEFMA
                     LAASQSNFRYLPFVNEGPESNAETVDVIKQIAGNGRRVTPMIAGVKAFARPLRALLME
                     LGFDRREVKLETYD"
     gene            33055..34470
                     /gene="gltX"
                     /locus_tag="PK224_09195"
     CDS             33055..34470
                     /gene="gltX"
                     /locus_tag="PK224_09195"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRB15953.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLFARQQNGVFVLRIED
                     TDQSRSTDDSIQAILDGMRWVKLDWDEGPFRQTERMDLYREHAMRLFEQGHAYWCVCS
                     SEELEARRKEAETKGGSPKYDGRCRNLGLTKPTGEAALRFRAPQDGQTVVDDLIKGPV
                     VFDNQILDDVIILRSNGYPTYNFSVVVDDALMGITHVVRGDDHLTNTPRQIPIFQALG
                     FPLPRFGHLPMILGPDKARLSKRHGATSIMAYKDMGYLPDAMVNYLVRLGWSHGDQEL
                     FSRAELIEKFSWKNVQTSAAVFNPDKLLWLNAEYLKTSPPAEIAQALVPLLEQAGLKE
                     EVRAVSTEWLAQLVVLVKERTKTLVEMVHWVTPYFGQAVVCDEEAAKKFLTATHAPVL
                     AKLLSRFEAFPTFSKQEWEESFKQLVEEEGIKMGQLAQPVRVALTGRTASPGLFEVME
                     VLGRDRTLLRLRKGIEQASRS"
     gene            34541..34614
                     /locus_tag="PK224_09200"
     tRNA            34541..34614
                     /locus_tag="PK224_09200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34573..34575,aa:Gln,seq:ctg)
     assembly_gap    34616..34715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(34841..36685)
                     /locus_tag="PK224_09205"
     CDS             complement(34841..36685)
                     /locus_tag="PK224_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRB15954.1"
                     /translation="MAPNILLSCESISKSYGVRALFTDLSLALSDGDHVGLIGPNGSG
                     KSTLLKILVGLESPDEGTRTLRRHTRIGYVPQEPLFPPHHSIEQVLQDTLTEDGLDPH
                     EQGGRIARALSIGTFPDPEQAVSTLSGGWRKRLAITQALLLEPDVLLMDEPTNHLDLE
                     GIIWLEQLLKNRAKAFLVISHDRRFLESVTSRMVELNRCYPEGRFESRGCYSDFLEQR
                     DAALQAQADYQASLANRVRREVEWLRRGPKARTTKAKGRIQSAGKLIDELNQAESRSA
                     QSTIGIDFSASGRKSKQLVVAEQVSKSFNGTPVITNLDLLLGPGQRLGLLGPNGSGKS
                     TLLRLLAGTLEPDSGTITRADALRIVSFEQHRESLDQSTSLRRALAPSGDAVVYQDRS
                     IHLASWAKRFLFRPEQLDLPVSKLSGGEQARLLIARLMLQPADLLILDEPTNDLDIPT
                     LEVLEDSLLEFPGALVLVTHDRWLLDRVSTILLALDGTGRVDWFADYAQWESAQERAG
                     GSAPESKSDGRAAVQDETASTSPARKPKKMSYREQKEWGTIEESILKAEEEVTACQAA
                     MQDPAVLSDAAALQARSQALVAAQAEVERLYARWAELDEKRAQTVQSS"
     gene            complement(36835..37170)
                     /locus_tag="PK224_09210"
     CDS             complement(36835..37170)
                     /locus_tag="PK224_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15955.1"
                     /translation="MVTGPCSEEPLEEPGSFRERLSLLEAAIPNAATEAQPLLRHIYR
                     STRRLHDQLQRIDPTPPLPRSVVSEVRCFDCGTTKMESFHTSTQGGYHLCPSCFQQRL
                     RTGRARTAH"
     gene            complement(37324..39027)
                     /locus_tag="PK224_09215"
     CDS             complement(37324..39027)
                     /locus_tag="PK224_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRB15956.1"
                     /translation="MSTTPEPPTRSDFIREIVAADRAAGKHEGRVVTRFPPEPNGYLH
                     IGHAKSICLNFGIANENSGGICHLRMDDTNPTTEDPEYVQAIQDDVQWLGFDWQDKMF
                     FASDYFERLYDYAVGLIQKGLAYVDSLTADDMRKYRGTLTEPGQASPFRTRSIEENLD
                     LFRRMRAGEFPDGTHVLRAKIDMASPNINLRDPVLYRIRHVAHYRTGTAWCIYPAYDF
                     AHPLSDAMEGITHSICTLEFEDHRPLYDWVVEHCGTAQRPQQIEFARLNVTFTVMSKR
                     KLLDLVERKLVNGWDDPRLPTLKGLRRRGFTPEALRAFCEAIGVAKRDAVVEMQLLEH
                     FVREDLNKRAPRVMAVLRPLRLVIENYPEGQVEQLEAVNNPEDPSAGTRQVPFSRTLY
                     IDQEDFREDPPKQFFRLSPGREVRLRYAYIITCVGVVKDPATGEVTELRCTYDPETKS
                     GGAQAQRKVKATIHWVSAAHAIDAEIRLYESLMLMDPGKIPADQDWTQHLNPHSLERL
                     PTCKVEPSLAAIAPGTRVQFERVGYFCADPDSSPDKLVFNRTVTLKDTWAKIEKAQTP
                     R"
     gene            39226..40383
                     /locus_tag="PK224_09220"
     CDS             39226..40383
                     /locus_tag="PK224_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15957.1"
                     /translation="MMNGRWSACGLVVLLGILWAGGSQAVEPDSLEACRAQPFDAKMQ
                     CYQRHLETVLQAEGTEQTLTVLEQITVQDPEALREAHPLVHHVGQRSFARYGTAPLAL
                     AHCRDVFWSGCYHGVLQAFLSSLATVEPQHILPLCPISETVSAYSFQRYNCLHGLGHG
                     LTIQFRYDLLKSLAFCDALPGSWDRESCYGGVFMENIVTFQQAQRAQPAQTGSEHHHH
                     EATSFLNPQDLLYPCSVLNEKYLRACYLMQTSAVLTFLNYDFAQAFMQCARVEGEHQT
                     TCYRSLGRDISGYTLRDAVRVNELCRLGQADQIRQCFIGAVKDFILTDANPDPGLALC
                     RSLDGPFKKDCYATVGEMVIPLFDDKEKRAQACRKGEDEYVDACLAVATAF"
     gene            complement(40619..41032)
                     /locus_tag="PK224_09225"
     CDS             complement(40619..41032)
                     /locus_tag="PK224_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15958.1"
                     /translation="MTDVSPPSVQAFLVCDCVIEDSLTKKKSLIGLFSHLQSVTFPFQ
                     HHQLGLYFCITDAEGTYHVEIDLMFVNTDQLVCRASLPDIAIGDRLQISDFGINIPAL
                     LFPAPGRYEFRLRMNGRMIAQKDFHVVQVSAPPGT"
     gene            complement(41048..41572)
                     /locus_tag="PK224_09230"
     CDS             complement(41048..41572)
                     /locus_tag="PK224_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15959.1"
                     /translation="MELRKPDPAHSSHEAESDRHKHSLEIRLGSNIFRNTNGVIRVQG
                     KEQLVLELAPDQERILLTIDLYDGSGNHVAHLRRNRWAFNDGNRFSLNTSESPPTLFP
                     NLPWLKVTDQETGETVLEAAVAPGEKIHVATGKFYSHRGQLIEITSHFCRIGSTHTLF
                     GDVFEARGGTAVLG"
     gene            complement(41697..42131)
                     /locus_tag="PK224_09235"
     CDS             complement(41697..42131)
                     /locus_tag="PK224_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15960.1"
                     /translation="MVLRSTRLQTVLIAGLLPAFLSLAGCGASFLEGTIAPPYSSAWT
                     KCGATTKVRVKPPHSTVTVAYTEPTTGTDGKPLTNLAYTTIYYNAGDGPVIGKVVPAS
                     GKTGGAAVSQVVMIPLRDQSEQEVTVCVTATDSDEREGPASP"
     gene            42303..42380
                     /locus_tag="PK224_09240"
     tRNA            42303..42380
                     /locus_tag="PK224_09240"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42339..42341,aa:Glu,seq:ctc)
     gene            complement(43574..43855)
                     /locus_tag="PK224_09245"
     CDS             complement(43574..43855)
                     /locus_tag="PK224_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB15961.1"
                     /translation="MTPEAAHTHSVPEIMTLAEAAAYLRLSKSTLYQRKDIPRHRIPG
                     SREVRFLRDELLDWLKSGDVVAASRGPEVLTFPLDRNAGTVYHRNPRYR"
     gene            complement(43852..44166)
                     /locus_tag="PK224_09250"
     CDS             complement(43852..44166)
                     /locus_tag="PK224_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15962.1"
                     /translation="MQLAEDLGRAQARRLAVRRIETALGLITTGLAHRDRQIRDAILI
                     ELAGTTAHGELLKKLDELLDAIASNVNRLGRRLRDGFAYDTLLELQTEARAIFLRLDG
                     AR"
     gene            complement(44168..46876)
                     /locus_tag="PK224_09255"
     CDS             complement(44168..46876)
                     /locus_tag="PK224_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15963.1"
                     /translation="MGDTGSLIMRLKMDASNMEKELNRAARNTDDFGKLVSSAAKIGT
                     LALGALTAVAATTGAALISMTKSSAVVGDELNKTSQKTGVAVETLSGLRFAADLSDVS
                     MEQLSMSMGKLARAQQDAATGSTQARTTFQALGVTYKEADGTLRPLNDVMLDAADQLA
                     RMEDETKRAAMAQEMFGKSGMQMLPLLNQGSAAIRAQMEEAKALGVAWTTDAAQGAEA
                     FNDSLTRLGYVAEGFMNDVAQVLIPILTDVTDGVLNWVKANREWIKDEIQKDVRALVS
                     AATDLVSVMGQIGKTSIEIKNSAFTDHLTLGMKALGYVVVSTFTMMIGMLEQFALNIT
                     KLGALLPEILGGKIFQEHANPLEAMLAKNRHQLNLYTEKIAMDFGLMSKNSIGPIEVV
                     TKKVEQAVQAVKKASHAMKAFPTVQGANQDAAALDFYAAEREQALGNLQLIQDLTAAR
                     EELASGRAEEQLGQFINARYKDEQEMAKQTAALQHSLMLEHLNDRAKQITVIEEEAAT
                     RARFVKENIQDETERAALLEGIERTKSMRLSALKIEDQKRELDLFEELRQAAMTGEEA
                     QRYEIERTAEARRRQVVETIKDAQLQAMAIENIEIAKTNKLNALGVQHKSFFTEQMDA
                     IRQSAAFTWSSIVQSFSQAIVGMLQGTKTFADFLQSVWTTILTSFVNMAVQATLAFIL
                     EQGKQLIFGQTAASAQLTQHTALEAAKTAATATAETSRLAITVLTNKAMLAASTASIV
                     AIGATGNAALATLGIVLEAVVGFMAAVAAAVAAAPVVGQALAGSILSAAFIAQFTGAA
                     ALTAATAALNTALGGAIVASTGALATPFADGGIMTGPTIGKFAEAGSAEAAIPLNDRG
                     AKFMQSAMGFGGTGTQQINLYVDGRLMTKQVVKHMPDVIHTKLGYT"
     gene            complement(46869..47216)
                     /locus_tag="PK224_09260"
     CDS             complement(46869..47216)
                     /locus_tag="PK224_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15964.1"
                     /translation="MTEHLPGRTTPRSLPIQLSGPRQLLIDAGAIRLATDQFGLFKNS
                     RPLEYPVDLPFTQHMIWAGLQRGGPALRLKLVRRLITQDNYERFGFLVIKAICAGFGY
                     PYEDVPFEEVKNG"
     gene            complement(47577..49355)
                     /locus_tag="PK224_09265"
     CDS             complement(47577..49355)
                     /locus_tag="PK224_09265"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRB15965.1"
                     /translation="MVLSSSYCSEPGPWRTSRVPYLREIMDALSPNSPVREIAFMKPA
                     QVGGTAAATNWIGYTIHRAPCPMLLVEPTLDMAAKLSKQRIQPMIETVTCLDGLVRES
                     RSRDSGNTIMSKEFTGGILCLTGANSGPGLRFMSAKNLFLDEVDGYVPDVNGEGSPCE
                     LAEKRTLTYARHKIYRCSTPLLKRSSVIEKAYLESDQRKYFVPCPHCDHGQVLWWKNL
                     AWPDGKPEEAKFLCEHCHQYIGEAHKPRMLADGKWLAEAAGNGGMLKAAGFWINALYA
                     PYGWVNSWAKLASEWTEIIHNHDFRAQQTFTNTNLAETWEEAGERIEESELHNRVEIY
                     PAPVPNGVVVLTAGIDVQKDRIEAELVGWGKGQESWSIDYRKWFGSPTDKTLWDQVDE
                     WLKRTWDHETGGFLTVATACIDTGHHAKEVYEFVLPRQNRRIWAVKGSSQPGAPVYKM
                     GSTVNGIRLFLAGTDTSKDTLFDRLKLEDFGPGYCHFPNLTAYTEDEFEYFKQLTAEE
                     RVNKWERGVLMGSYYRKKRSRNEALDLRIYAMTALAILNPNLDTLTVMRHEPPPAPAP
                     VDPDRSSWVHRNPEARPRTSWVKRDR"
     gene            complement(49542..50030)
                     /locus_tag="PK224_09270"
     CDS             complement(49542..50030)
                     /locus_tag="PK224_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15966.1"
                     /translation="MDLFGTVTWTRQELAGVLGLTPMRITQLVQEGVLPKPVRAHQHE
                     PKAAVTAYLKYVQQNKTSGEREKEETEKLRLGNAMTRLKLDRASGELMSRDAVNQAWF
                     AAGRQVRDTLENLPDRLAGPLAAETNQAAVFSLLKSEFHQVLEMLSRLPDVKAKKILA
                     AV"
     gene            complement(50154..50762)
                     /locus_tag="PK224_09275"
     CDS             complement(50154..50762)
                     /locus_tag="PK224_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15967.1"
                     /translation="MSAGIPSTLHSLDGYGPAGDGFANKENQQMAPTQIIMGGVSAKL
                     DEARKVLRLNAKHFNTVEPLKRAELHLHPEEPLDGIVRGDLVSALDGKCCSFDETLPF
                     AGIAENVGQDNKSRVIVGVFTRGCFHGRVEGLGPESRRGTLVYGEPGERRQPLNVLGK
                     GVLIGALFAVESLERGIGIICFKASNDEEPFLNARNEGGPLR"
     gene            complement(50759..51157)
                     /locus_tag="PK224_09280"
     CDS             complement(50759..51157)
                     /locus_tag="PK224_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15968.1"
                     /translation="MINDQVFTKATQLLEPILNDFSNSLASSIAQILSQRRKHVQPTT
                     TSQDAQRLQTLRATMARDQQETLRAMNETAWSDAALAKIPTLDLLHFAYEHSVTLRQE
                     FGTIERFEAYWRNLCRQKTRRVARDEQGKR"
     gene            complement(51170..51433)
                     /locus_tag="PK224_09285"
     CDS             complement(51170..51433)
                     /locus_tag="PK224_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15969.1"
                     /translation="MSERQNNSSERAIAQFRQQLSAHFSNALQFEAVDLTESDVLLSD
                     FDIRLRAPWLSGLTKPARRGPVDLLVGRSSLENRTTRANHDGF"
     gene            complement(51430..52020)
                     /locus_tag="PK224_09290"
     CDS             complement(51430..52020)
                     /locus_tag="PK224_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15970.1"
                     /translation="MPSPKRWHPVSRDLNDDAELWAFTEEFGDRAIRIWLEVLAILDR
                     TENHWRLNGDWLGTISRKVRLYPKKVSASVLWMTKKGWLSVKEQDSDGSPLVLESPNY
                     WKYHKRRDAKAEMEGADKGTIMAPSYPNLPFPNHPNRKEEKEILRSGSSEQERGRGRN
                     AVGRAMREEDLPPHLREVEEFSPLGSTLKEICRRLH"
     gene            complement(52318..52950)
                     /locus_tag="PK224_09295"
     CDS             complement(52318..52950)
                     /locus_tag="PK224_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15971.1"
                     /translation="MSKYDKTKGKTDTRQLAVWVYLIAVRELYWDSRPSYFQMTEKDI
                     ESRHAELFGSLLRKISTGHKDGGNHLRESVVLQRNWLDWFLHTNGYDNCKNAAQDRWH
                     WLRKNRGHIFEGLRLFPCYCPYSDELKIPSLKEFKKNNEQTIQRADFIAETLSMIHNA
                     ASAVVIKKHYRPSSIKPNDSAPRYKKIVDNEGVVRAVLTDSSPPFWPWFD"
     gene            complement(53196..53432)
                     /locus_tag="PK224_09300"
     CDS             complement(53196..53432)
                     /locus_tag="PK224_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15972.1"
                     /translation="MSEKKYRAVSFQLPEDVVEIYEELQRQTPISRRANAASNLERQL
                     IWAIAKYHAEEGLLNLGVSQKKVSAAVPVLSLVP"
     gene            complement(53778..54008)
                     /locus_tag="PK224_09305"
     CDS             complement(53778..54008)
                     /locus_tag="PK224_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB15973.1"
                     /translation="MSDLMSLSCDTPVEGLQGILGNFESAQIGLRNLRDRLVDSGEQG
                     LVEDASIFLGLYVSYTDELIKKAYREVSSLPK"
     gene            complement(54005..>54550)
                     /locus_tag="PK224_09310"
     CDS             complement(54005..>54550)
                     /locus_tag="PK224_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB15974.1"
                     /translation="QAPLKEWPRLSNQPEPPLSSLAGFFLRGTQPRIGGRYFSQWKDA
                     GSFKHRWIEATERAGVHDLHFHDLRHTAATWLGEARVEYATIEKLLGHRLPGMGEQYI
                     HDWKTKLRAAVTALEQTVMERFREAAQDERVREFKPCPMPAGCTVSNQRPPWWIESAE
                     QQNRAAFTSLSPPLQLKGARQ"
BASE COUNT        10766 a        14670 c        16479 g        12535 t
ORIGIN      
        1 tgacgataca ggaactgttc aagaccttgg ggttgacgca ggaacagccc gggggatgtc
       61 tctcctcttc gacgtggagt cgcaccaccg acgccggtat cgtcgagtcc cgtaatccat
      121 caactggaaa cccgctggcg cgagtgtacg ggtgttccct tgccgattac gatgccctcg
      181 taaccggatc gcagcaggtc caggcctcgt ggcggatggt gccggcgccg aagcgcggcg
      241 aggtgatccg tctgataggt caggcgctac gcgtgcacaa ggatgccttg ggtacgctgg
      301 tgtcgttgga ggtgggcaag attaaggctg agggcgatgg cgaagtgcag gagatgatcg
      361 atatggcgga cttcgccgtc gggctctcac gcatgttgta cgggcagacc atgcattcgg
      421 agcgtgcacg gcatcggatg tatgagcaat ggcatccatt gggtgtggtg ggtgtcatta
      481 cggcgtttaa ctttcctgtg gcggtctggg cctggaatgc ttttattgcc gcggtaaccg
      541 gcagcaccgt gttgtggaag ccgtcaccga aagctccact ttgtgcattg gcggttcagc
      601 ggctgtgcaa tcgtgtgctg gaagaggccg ggtatcccgg tgttttcgcg ctctttacga
      661 cggatcgcgt cgagctggcc gagcggatgg tgcgggacga gcggcttccg ttgatctcct
      721 tcaccggctc ggtgcccgtc gggaggcacg ttgcggaggt ggtcggacat cggctgggcc
      781 ggagtcttct ggaattgagc gggaacaacg ccgtcatcgt ggacgaaaca gccgatctgg
      841 atttagcgac gcgcgccatc gtcttcggcg cggtcggtac ggcgggacag cgctgcacca
      901 gtaccaggcg actgattgtg catgaatctc agtacgagct gctggtcccg cgactgctgt
      961 cggcctatgc gcaggtgaaa atcggtgatc ccctcgagcc ggatatgctg atggggccgc
     1021 ttatcgacgg ggaggcggtg acgcgttacc tgtctgcgct tgaggaagtg aagaaggcgg
     1081 gcggtgaggt gctatgcgga ggatgtgtct tgtctcgccc cggatatttt gttgagccca
     1141 cgatcgtgcg ggcgcagaac cactgggata tcgtccagcg ggaaaccttt gcgccgattc
     1201 tctatgtcat gacctatcga acgctggacg aggccatccg gatgcacaat gcagttccac
     1261 agggactgtc ctccgcgctg tttaccaccc atcttcggaa cagtgagacc ttcttgtccg
     1321 cgatcggcag cgactgtggc attgccaatg tgaacatcgg gacgtccggt gcggaaatcg
     1381 gcggggcatt cggtggagaa aaaaccacgg gtggcggacg ggaagccggt tccgatgcgt
     1441 ggaaggccta catgcgtcga caaaccaaca cgattaattg gggaactgag ttgccgttgg
     1501 cccagggcgt gacgtttgga cgggcggaag gagggaatga tggggcaaga agctgacctc
     1561 gatgcggtga tggctcgtat gacggccgac tttctggcgc agaatagcgc aggacggatg
     1621 cttcgccggg cgctggacga cgtcggggtt gggtttatcc cggtgatcga ccatgtcacc
     1681 atccggacga tggatattga tcggcgcgcc gaggaatttc tcgcactggg ttatcggtac
     1741 gatgaaacgc ttcattttga cgattggtat gccaaagtat tccggtgggc cgggtatccg
     1801 gcgctgtttg tcgatcaggc ctatccggac gaccgtggca agaccagcat catcccccgc
     1861 tgggtcgaga aattcggtga tcaggtgttt caccatgtgg cggtccgtgt cgaagacatc
     1921 gagcaggcca ttctcaaatt gaaggcgaag ggcgtggtct ttgccggcga gatcgtcggt
     1981 gcccggggag gcacgttgcg ccaaattttt acagcccctg agatgctcga tgggcaaccc
     2041 ttttccgtcc ttgaattggc cgagcggcac cagggttatc agggatttct tcctccacaa
     2101 gctgacagtc tcatgcgctc gacaatgcga ggcctgtaag gccgtaggct tcggccgacc
     2161 gctgctctgc tgtcgcgatg tgttggcagc ctcttcccgc cgcccaaaga gtgcctcgac
     2221 cagatgggtt gaattgcgta caatgcgccg tgtcgaccaa ccaggtcgag agtgtctggc
     2281 atgcgcgatc tcaacaggag gttctcttca tggtgcaatc taacgcgacg ttacgcttga
     2341 tcctcgcact gctggtgctc tgtgcacttg gagcaggaag tggttccgtg caagcatcgc
     2401 cgtccggcca tgcccctcat ggcaccgtga cgatcgatgg tgtcacggtc ccggatgtcg
     2461 ggcccctgcc gacggtggtc ccgataccgt cctccaatct caactacacc gctaagatcg
     2521 aactcggcaa gcaactctat tttgacggac gactctccaa aaataacgcg atctcctgcg
     2581 ccttctgcca caaccccggg accggatttg cggatccccg ccagacgtcc atcggagtcg
     2641 gcggaggcgt cggaggacga cagtcaccta cggtctacaa tacgggattg aatcacgttc
     2701 agttttggga tggtcgggca cgttcgcttg aagagcaggc catcgggccg attcataacc
     2761 ccgtggagat ggccgagacg catgaacatg tggtggccaa gctggggaag atcaaagggt
     2821 atcagcaaca attcagggct gtatttggaa cagatgtcaa tcttcagggc attgccgagg
     2881 cgatcgccgc ctatgagcgc acagtgctct ccaccaattc cgcttttgat aaatacgtgt
     2941 taggcgcgca gaaagccatg gatgaggcgg cggttcgagg actggccctt tttaagggca
     3001 aggctcgttg catcctctgc cacaatgggc cgaacttcac cgataatcag tttcacaacc
     3061 tcggcgtgcc tcaggtcggt ccgatgaagg aagaccttgg gcgttttgct gtcagcaggg
     3121 cggagaaaga ccggggcgca tttaaaacgc cgacgctccg tagcatcaca gaaaccgctc
     3181 cgtatatgca cgatggcgcg tttaaaacgc tggaagaggt cgtcgaattt atggatcagg
     3241 gcggcgggag caatcccaat ctgagccccc tcgtcaagcc gttgaatctg acggcggagg
     3301 agaaaagcga tctcgttgca ttcttgaagg cgctggcagg agagccgatc ccgttcagta
     3361 tgccacaatt gccgaaataa aacacgaatc cttttgggcc gcctgtgact ctcacgggca
     3421 gatacgtgtg ccttggtcag actattttgt ccaagcatcg gtacgttcca accccaacca
     3481 taggaggcca tctatgtttc gaaatctttg tgtgctcggt gcgagcacca ttgcgctcgc
     3541 cctcggcggg cctgcacttg cggcggatat gaagccgggt ccggcggacg gattcgacat
     3601 tcacgtcatg gcgccccata aaatggaaga tgggtcggtg gcaggcccgt tccatcacta
     3661 ttgtaaggcc gtcaagccgg aagtcttgca atgtctgctg ttcgaatcca cggatcccaa
     3721 tgccgtgctc accgacgtgg agtattttgt ggccaagcct gtgtcacggt cggcggtgcc
     3781 tctcgatgtc tggaacaagt tctatcacga ccatgaggtc gaaatagcga ccgggcgtgt
     3841 tcaggtgctg gatatgcctg atgctcaggc caaagaagtg gcggcggctg cggccaagac
     3901 ggatggcatt attttccact tgtggccgaa gggtgcgaag gctccggatg gatcggtgga
     3961 gcatccgacg tccgtggggc ataaaccgcg gacggagtaa gtctgcgaat agcgcgtgat
     4021 ccatcctgtg ccctaattgt aaggaggcca ctatgcgaag tgcgattttg atgtttggat
     4081 tgtgtgcggc ggcgttgacg gcagccggtt gtgcggagac tgcccgcgaa atgaaatcct
     4141 cctctgcggc accagctgcc gccacggcgg cgaaagctat gccgacaccg gcgcagggct
     4201 ataccattca tgtcatggct ccccacaaat ttgaagacgg atcggtgcat ggtccgtatc
     4261 atcactactg caagccgatt tccccggaag tgctgcagtg tttgttgttc gagtcgacgg
     4321 acgccaacgc cttgctgacg gacattgaat atttcgtcgc aaagtccgtc tctcgtgcgc
     4381 atgttccgtt ggagacgtgg aataagtatt accacgacca cgaagtagag attgccacag
     4441 ggcgggtgca gattctggac atgccggagg cgcaggcgaa ggaagtcgct gccattgcgg
     4501 ccaaaaccga cgggatcatt tttcatcttt ggccggacgg agccaaggcg ccgaacggtc
     4561 aggtggggca tccccagtct gtaggccaca agcatcgcac tgaatagccg gcgcgacgca
     4621 gatccatcgg gcggaggggg cacagccctc tccgcctgtg ctatttctga tgaaagggag
     4681 acgctgtgat ggatgctgga cgtctatgcc tgatgggatg gctgctgctc ggcggtgtat
     4741 ctgttggttg ggcggcgaat tccgaagtgt taaggccccg tgttccgatc gaccagatcg
     4801 aggcggccag gactgtaacc aatccctttc ctgttacacc ggaaatgctc cagcagggaa
     4861 aggcgctctt cgaaggcaaa gcattctgtc gcgcctgcca cgggtcggat gggaagggac
     4921 taggaatgga tttggactat tccaccttca aaggaccact cccacggaac tttactgata
     4981 ggctatggca gcaggccagg acggacggtg agctgttctg gattctgaag aacgggagtc
     5041 ccgggacgga tatggccccg tttattccac tcgtcctgac cgaggaggag gcctggcaag
     5101 ttctcatgta tgtccgttct ttcggtgggc gatgacgcgt gggcctcgtg ccgcactcac
     5161 ggtttcaatg tcgtcggtag gctcggttcc ttgcgggcgg cttggttgtc gggatgccgc
     5221 aagctcgccg cgctgagaca ggtgaatggt cggcattgac cgaagctgag gcgcggtata
     5281 ccgacaatgt gtctgactgg tccgttacgc ggcgcctgca ctatcaaagg atctcagcca
     5341 gccgacgagc acccccccca cttcacaaga tgttgccgag agtctcttga tgtccggcgt
     5401 gtctcgagga ctgcttttgg ctagaccgaa tgctcgatga acgcaaccgg cgtcatcaag
     5461 accgatcata gttttcttat tcactgaaat tatcgattgc aggcaagaag gcgttggccc
     5521 cagattggat cgctttttgt gcctggtgca gggtttctct tcgtatcttg ggtaggtcta
     5581 tcgacggaag agctttacga ttgagctggt aggagatagc aatcgggtcc tagttgagac
     5641 gacctctgtc ggatccgcgg aactgcattg tcggattggc ccagccgcgt tgggacccag
     5701 gcattgcagg gggatcggcg agccttcaat acggcggtcg tgattttata gtaccaacac
     5761 ctccgtcggg atacagcatc gactttgggt tgtgaggaca ggcgtttgtg aaggtgttgt
     5821 tgagacgtta gaagatcagc gctggtgggc caataggaaa tgagctaagc gggcttgatc
     5881 agaagtaggg agcttgagga attcgatccc gaagtattcc tccctgatcc atcggacggc
     5941 ggcgagttca atgatcagtg cgtgggtggt atcgggcagg agcaggcgca cagccatata
     6001 gctaccctcc aacattctcc ggtcgcattc cactaaacat cccgtttgag acaggttgtg
     6061 tataaggccc tctccgataa agagggcgcc ggagaacatg acaggacaga cgattctaag
     6121 gcggtctcga gaccgctgaa atatgagaat tgcttcggat gaaggtgggc ggtccagagg
     6181 gccaagtgat gctgaggagt agtggctcat ggaagggagt atagttccga gacgcagtgt
     6241 ggcaacatcc cgccttggag gggccagaga ctcgttggat gaagtcgatg cgggaattcg
     6301 aatgacttgt gatgacaggt aattcaggga cagttcatcg gcggcttgcg gcataaaagc
     6361 ggtggctgca ggcccgacat cgccaagggt aacgaccaag cagataccgg acgaggtctc
     6421 gccaacctcg acgttcagaa cgacggatga ttccggtaca ggcggggcag gaataagctg
     6481 tcttggacag ggtttccagt gagcgccgat cgcccaagaa agagatcatt tagtgagccc
     6541 ccgtttgaga gcttcgcact ggtcgcggga gtagtcgtgt agcggttcac aggctgcagg
     6601 tttcgggctt tcccagttcg ccatgcaata aaatcgctct cgatagctga tggtgagtaa
     6661 gtcgaagggg ctgggtcgag ggtgttccgt ctgttcacgt cggcggtcgg tcgctgtctc
     6721 gttgtgagca gcgctcagat gcctggcgtg aggacgcatt actcctgcca ccacaccagg
     6781 cgaatgagat agattcctgc cacataagtt cccgcgatga cgatggccca gagcatgatc
     6841 gtgcctcctc tttttatctt ttctgttttt gccggggtgt cgctgccata acgactctga
     6901 gcagtagcaa ctcgcgtacc actttggtaa tgcgtcccaa tctcggttaa acacattgat
     6961 tcgcttgtga ggagtgccgg tgagatcaat cgcgttaagg cagcaggtct aggcaaatgg
     7021 gaccaaggtg tatccgattc gataatgaca gcatcaattt ggataacgtt gggaagttcg
     7081 acttgaatgt ggccgtgggt tggctactcg tggctgggcg aatttatggt gtgggcggcc
     7141 agaattgccg tgaggaacat ggctctgatg agccttggat ggcacatgat tacttcgccc
     7201 gggagtgaaa gcggtgacca aactggtgcc tgcctcgatc aaggcgcgcc tggattttgg
     7261 tcgccggagc tagttcgagc ccaaaagtga actcgtaatt gcgaccagaa tcatgaatag
     7321 cgatgcgatt aagctgagtg ccgccggcaa gtcactctgt gctctgcgta agaggtcttt
     7381 tcggacggga tttttctcag ccattatgat cgtagttgaa ttacgcaaga ttctgagctg
     7441 gcgaagcgcc ttttcgaggt tgttgttcga cggccaaggc atgggctgtg atagcgtagc
     7501 cgcagtggcc gcaagataaa aatccacccg tgggggcgag gctgtgatgt ttacagcgtg
     7561 gacaatcata gagcttcatg gcggtttcct ggattagtgg cagaagtcat tgtgtagtgt
     7621 cgggattgtg ccggatggtt gtggctgccg taactcctta taccgtgcgt gtaggtgtgc
     7681 cacggcgttg gcgaacgatg tgaactcgtt ggactcaaaa tgtagggtga ctgagccata
     7741 cctgaagtga agttttccgc aggtgcacat gttgagtgcc gtctggccat tgcgcggatt
     7801 cagcgaatgc atgggttgtg agctcctttg ttgtatggga gggcggtttc gatgatcggt
     7861 cgtcagacgg ccgatgattc gcaataaaga ttcatccgga ttaggactag ggatgtcgtg
     7921 tgattcgtag atcgtctacc gttatgttgg atatggtgcg gcgtgatttc gcctgcctct
     7981 cgcctgagga gtatgagcaa agtgggctat gggccccgca attgatacac ctccatcgct
     8041 cctccggatg atagcagtca ttggatggct cgctggcaag atatcctcct caacgagaac
     8101 aactcatacg ttgcccctcc agaataaagt gttctctgtg aagccgctgg gtggagatgg
     8161 taaaggggtg ctcccttcac cgtctccacc cagcgggtcc attcacgcta ctccgtcgac
     8221 ctgatgggct tgatcagtgc cgccttgtct tcaggcgagg gacataaacg ggcagttcct
     8281 ggtcgcgctg aagattggag cagggcgaat gaatattatg atttcaattc tcaatatcag
     8341 tatagtgggt tagtgagatc tcgggaatgc ttattttcct gatcttcaga taatttcttg
     8401 gtgctgagga tggcgcgggc ctgctattcg aactgcgaag gcgaggcctt tctagcaggg
     8461 aacgaaaaag agggagctaa aacgcggttt gggaggagtg cggtggtcgc ggccggctca
     8521 gtcagtgaaa cttcttcatc tcctagccgg gagagttgaa tctacgccac gctcagcatg
     8581 agaaggagga acgagtctgg tcatctgtgg cgctcgttgc gtcttgcgct ctgcaggcga
     8641 aaatcgaggg tttccatttc cacgacctgc gacatacgtt ttcgacgatc ctggtacaga
     8701 gcggaatcga tctctacaag gtgcaactag ttgctggggc acaaatcgcc cgtcatgacg
     8761 caacggtatt cgcatcatta tctggagagt ttacgaaatg aggtagaagt tttagatcgt
     8821 atgcgaggag ctaggacaaa tttagcacag ccggcgggtc ttgaagaggg gttgaactgc
     8881 gtaagtgatt ggaaataatg gtgggccgtg taggggtcga acctacggcc cgctgattaa
     8941 gagtcagctg ctctaccaac tgagctaacg gccccacctg tgtgcactat gtttcgatcg
     9001 taacgtacct gacagagcga atggtgcgcc tgacaggatt tgaacctgtg gccctcagct
     9061 ccggaggctg atgctctatc cagctgagct acaggcgctc ccgtcgatca gatccgacac
     9121 attagcatag cgtttctgtt gctgtctagg gagttggatc accatttggc acgaatccat
     9181 agggtaggag gatttcatcg tcgcccggag gttgagctcg gcgcaggatg gacaaagaga
     9241 gtgcctcctg ttcggccagg cggtcggcat cgcgtcgaag ggtggctgag aggcggacaa
     9301 tgggcacggc ggtttggacg gcacaagcac catggactat cccgtccgat tcgccgggaa
     9361 tgcccatcgt gcggcatgtg atgggtctgt gggagtagat actgcaggtt ccgtcggcct
     9421 ccagggcggg gcagggaagg tgggcgaatc gttcggctat ctcatcgatg gtgtcatctg
     9481 cccactcgtc aaggcccggt gtcgatttca accttgggta ggccgtttcc acagcggtga
     9541 tctgggttct ggcccgcgtg acgatggcgt gcgtgagggc ggaaggtaat tcatcaaggc
     9601 cacgttgtat ttcgagggcg tcgaggcgag tgatgggaaa gatgccggta cagcagtcgc
     9661 agcagccgcg cccgcagggg atctgcccca gcagtgaggc tcgtgcacgt ctgaaccagt
     9721 cctcggcttg ctggaagagg ggcaaggtgt gaggggagga cgccgtggcc tccatggtgc
     9781 ctattcctcg gggagagaga gatcgacgat gagttctcct ttgggggaga agaatccttg
     9841 ctgatcgatc tgcacgatgc cgttgcagtg ttcctggaaa aattccaaaa tccatccgtt
     9901 gaaatcatag ccctcgtcgg tgacgtcggc ttcgaccatg cgcgttgtga ggatgaaccg
     9961 cgcgcgggtg acgtgttctt gggccagact ggcctcgagg ctgtcgtgtg aggacagatg
    10021 ctccaggaat tgtttctgct cctgctcgaa caaatcttga tagctgcccc ggtcgcgcac
    10081 gcaaaacacg tgaatcggct tacgctcgcg cgcataaccc aaaatgacct gcacccatgc
    10141 ccactcgtcc agcatggcat cgctcatgtc gggggggaga atgggaagtt gtccccgcga
    10201 cttgaggaat tccatcaaca ggcgaagcgg tggagcattg tcatttttgc agaagactcg
    10261 tgagtaaatg accgcttcgg gtgcggcgcc ctcggctggt tgggcggtag agggaagaat
    10321 aggaagttct ttagccatgg cgacgcctcg tgatcctgag tgatgcattg aacagcagca
    10381 tgcctttcta ctctactctg tggctcacac ggcgcgcaag agcatcgagc gtgctggtcg
    10441 cctctctgga tgcgcgtgct ccgctttgcg gctcttgaca gcccccaggg ctttggatac
    10501 actctcgccc tgttttcgga ctgattctgc aggacactcg cagatttccg gctctacctc
    10561 tgtacgtacg agtttataga tcgcagaccg cacaagtcag ttcactttca ttcgttgttc
    10621 aaagggagga acgcatggcc aagtcgatga cgaaatcgca gattgctgac catcttgctg
    10681 gaaaggcggg gattaccaaa aagacggctg tccagttcct ggacgatttt gccgctctcg
    10741 cctatcgtga agcgaagaat gccttcgtag taccgggaat cggaaagttg gtgctggcca
    10801 atcggaaggc ccgcatgggt cggaacccgc aaacgggtga gccgatcaag attccggcca
    10861 agcgggtggt gaagttccga gtcgcgaaga tggccaaaga ttcgatcctc ggaaagaagt
    10921 agtcagcctc gttcctctat tagactgaag ggccggtgct ccgtcattga gagcaccggc
    10981 ctttgtgttt ttgcagcggc cataggtagc ggcgtgccaa tgagtacgtg gcggatacta
    11041 aaaagacggg gcgagtccgc ttccacctgc gccgtcatgt cccacgaagc gcaccgcatc
    11101 gccgtcttgc accagagaat cttcgctggc gatgcgtttg ttgatggtga ccaggacttt
    11161 tttctcccgc aacagttgga gcatgtgacg aagcccgcgt ccttgccgct gaataacctg
    11221 ccgaactgtc acaggggtgg ggagttcaca tgcgaggtcc cgctctccat ccgtggtttg
    11281 aagttgtccc atcagcgtaa tcgtaatcat gcgacctgtg tcctcccagt cgatctgccg
    11341 tagtgtttcg ttgtgccgtg cagccgagcg gcccaaattg actcccccag ccggctgcga
    11401 ctaatattat caccatgcaa tccctgaata tccagcgacc aggaatgccg gacctccagt
    11461 ttgccttggt ggtatcggcc ttgtgcacgt ccggcctgga aaccctgaac gtcccggagg
    11521 aacttcggcg gagagtgttc gacgcttgtt gggcgctcgt cagcaccgat cccccaccga
    11581 ccaaccctcg ggagcgtgtg ctggacttgc gattcggcac ggagctcacg ctggacgcca
    11641 tcgtggccac cattcgccag ctcttcgcag cagcggggat ttccatgctg acctgggatc
    11701 atgctcccag cgatccaacc cgaccgagta gtcctgctgc agagccgttg attgatcgat
    11761 tgcagaagct ctaccctgat ccgcctccga ctgctgatcc ctcagaccga aactagctgc
    11821 cattcttggt tctctattgg cttcgttcat tcaacatcag gttggggaag tggagggtcg
    11881 ctttccgcct gagatcgaac gaggtatgat gccatccgtt ctccccctgc gattcaggag
    11941 gtgtcatgca gattacgccc acgccgctta agggcctgtt tcagatcgag cctgatgtgt
    12001 tcggcgatgc ccggggaaaa ttcgtcgaga tctttcgcga atcacgctac gacgcggccg
    12061 gaatcgacaa accttttctc caggacaact tttcctggtc cgtacgtggc acgttgcgcg
    12121 gactccacta tcagctatct cggccgcaag ggaaattggt cacggtcgtc aagggaacgg
    12181 tttacgatgt ggcggtcgat attcggcacg ggtcgccgac gttcgggcag tggtatggag
    12241 tcgagctatc cgatagcaac atgcggcagt tgtatattcc tcccggtttt gcccatggct
    12301 tttgcgtcct cagcgaggac gcagggtttc tgtataagtg tacggatgtc tattccccgc
    12361 cggatgagcg gggcatccta tggaatgatc cggctctcgc cattgcctgg cctgtaaaga
    12421 ccccgctgct gtcggcgaag gatcaagcgt ataagtgcct ggccgacatg atgacggaat
    12481 tgcctcgtta tgaaagccgc tagtcctcgg ccgtttggtc ggccgattcc atggccacac
    12541 aactcttttt cttaggcggg tctccggcaa cgtgttgccc gtgtcttctc ttccccttcc
    12601 tttcgctcgt cgtccggaat catggactca ggtattttgt ggccttcctt ccgatgcatg
    12661 agaccgagcc agtcttgccg cgcgatgggc ctgcatgcta tgtaggaatc attcctgttt
    12721 agtgagcgag atcatgaagt tgaattcgca ggagattgag gcccggtttc gacggcacgc
    12781 ggtgcgtgtg actcgccagc gggcggccat ctatgcggcg ttggcggaga ctaccagcca
    12841 tcccactgcc gaggacctct atcgcacggt caagcgcgag catccgatga tgtcacgcaa
    12901 tacggtgtat tacacgctgg gggtcttgcg gcaagccggt ttggttcagg aagtaaacgt
    12961 cggtcatgag gtggcgcggt tcgacgggaa tgtcacggtg caccatcatc tgatttgtct
    13021 gggctgtggt cagattgtgg atgtgatgga cgacgggttg aatcatctgg ccatcccgag
    13081 tcggcaggcc gggggatttc atgtactcgg gcatcgagtg gagtttcacg gatattgtgc
    13141 agactgtcag caggcaggtc ttggcgcgat ggagcgtcgt gggtaggagt atcgattcgt
    13201 cacttaactg aaggaggaga gtatgggaaa cagtttgaag ggcacgaaga gtcacgagaa
    13261 tcttaagcac gcgtttgccg gggaatcaca agccaacagg cgctatctct attttgcgcg
    13321 ccgtgcagac atcgaagggt atcccgatgt gggcgggctg ttccgtgata cgtctgaagc
    13381 ggagaccggt catgcattcg gccatctgga ttttctgaaa gaagtgggtg atcccgccac
    13441 cggtgtgccg atcggaaaca ccgaagccaa cttgaagtcc gcgatcgaag gggaaaccta
    13501 cgaatacacg cagatgtatc cgggtatggc aaagaccgca cgcgatgaag gcttctctga
    13561 attggcggag tggtttgaga ccctggccaa ggcggaacgg tcccatgcca atcggttcac
    13621 caaaggcctg gatagcttga agcaaggcta agacctgttc gacgtcagtc tttggatatc
    13681 ggcgatcagc gagggatcga tgtgatctcc tccgctgatt gctggttgcc gatggctgcc
    13741 tgcatgatgg caacgcgcct atgaaccaat tcaacctcat caatccgatc gatcccaagg
    13801 acctggaaaa ggaaaccctg cggatctacg atgtgtgcga cggctgccgc cggtgtttca
    13861 atctctgccc ctcattcaat acgttgctgg atcgaatcga tgagtacgag agcgatgtgg
    13921 cgaaatttac cgcggcggac catcatcgcg tcgtcgacga atgctactat tgcaaactct
    13981 gtttcaacca ttgcccgtat acgccgcccc atcaatatgg catcgacttt ccgctcttga
    14041 tggcggtctg gaagaagcgg ctggcaacgg aacggggcac ccgctggcgg gactggctgt
    14101 tgacgcggac cgatctgctg gggagagtga acagcgcctt cgcaccgttg gtcaactggg
    14161 ggttgggtca ggggtggcta cggggtcttc tgcagacggt gctgggtgtc caccgggaca
    14221 ggcaggtgtt gcactatcag cgtgaaacat ttgtgcgctg gtgggctcgt cgatctgcag
    14281 gccgcaaggt aggcactgga aagaaagcgg cattgtttgc cggctgcttg gttaattacc
    14341 aggctaccga tgtgggcaaa gcgaccgtgc aggtgctcga aaagaacggg gttgaagtgg
    14401 cactgccgga ccagtcctgt tgcggaatgc cgtcattcga tatcggcgat acagcggcga
    14461 tggtggcggc ggcagagcgt acgatcgcct cactgaaatc atgggtggat cagggctatg
    14521 atgtggtcat tcctactccc agttgcagtc tcatggtgaa gcgggagtat gtcagtttgg
    14581 tggccggaga cgaggcgaaa cgggtggccg agcatacgta cgacatcagc gagtacctga
    14641 tgaagatgaa gcgtgagggg ggcttggcca tggatttcac gcagaagccg ggtcgtgtgg
    14701 cctatcaggt gccctgtcat ctgcgggatc aaaatatcgg tttcaaatcg aaggaactca
    14761 tggaatgcgc cggggccaag gtcgaggtca tagagaaatg ttccgggcat gacggttcct
    14821 ggtcggccaa ggtcgaattt ttccccctct ccatgaagat cgcgcaaaaa gccgtgcgca
    14881 tggtcgagca ggcgccggct gatctggtgg catctgactg tccgctggcg gggcttcagc
    14941 ttgatcaagc cggggccgtg gcccacgcgg gagggagatc ctcgcagcat cccatccaga
    15001 ttgtgcgaga cgcgtatgga ttacccaggg acccgcgtgc ctcctgaggg ttggcctcct
    15061 gctcgcggca ggagatgtct tctcaccccg gactagatca tgaaagcact cacacagcag
    15121 gacctgattc ctcacgatga gtatgagagg cggcgcgaaa cgtatcgtca ggccatcatc
    15181 gagctcaaac ggcgtaggcg gatcggtctt ggcgagaaga tcacgttggt gttcgagaat
    15241 cgggaaactc tgcgtttcca gatccaggaa atgattcgcg tcgagcggat cttcgacccg
    15301 caaaaggtcc aggaggagct ggatgtgtac aatgcccttc tcccggcctc gggtgaattg
    15361 agtgcgactc tcctgatcga actcacggat cctgacacaa tgaagcagtg gctcgatttg
    15421 tttatgggcc tggatcacgg ccagaaggtc ggcctccggg caggggggga cgtggtctat
    15481 ggcgaattcg agggcggcca tagccatgac acgaagatca gcgcggttca tttcgtccgc
    15541 tttcgtccga cccctgcgat ggtaacggcc atcggtgatc aggctgcgcg ggtggcgctg
    15601 acagttcgtc atgccgggta tgaggccgaa gccgaggtgc cgtgggtgat gcgacaggaa
    15661 tggcttgagg atttgagcac ggtcgggtaa tgttcatgga tgtgtgcgcg atgcgcaccc
    15721 acaataatcg tgcctgaacg ctgtcaaacg agaatcggtt cctatggcct cagttctcct
    15781 caacaaacgc attgagttct gcgcgtccca ccggtaccac aagccggagt gggatgctgc
    15841 gaagaaccgc gccacgttcg gcccctgtaa taacgatcct gggcatggcc ataactacat
    15901 gctggaagtg acggtggcgg gagaggtcga tcagcggacc ggaatggtcg tgaacctctt
    15961 tgatttgaaa cgcgtgttgt tgcaggtgtt ggaggagttc gatcacaagc acctcaatct
    16021 cgacctgccc tattttgagc gccaaattcc aacctccgaa aacgtcgcgc gtgtcctgtg
    16081 ggacaagctt caagcgcagc cggatatcgg taccctccaa cggctgtcgt tgtacgagga
    16141 tgaagatctc tgtgccgacc tcacggcaga ggccgggccg gaggtggcct cggtgacgag
    16201 gaggtattcg tttaccgccg tgcatgacgg ccatcgtgga catacctggg atctgttcgt
    16261 gtcggtgcat ggaccgatcg atcgtgagac cggcatggtt acggatattg ttgcgttgga
    16321 tcacttgctg aaagaacgcg tactggtccc gttcgacggg cgggatctgc gcatggtgtt
    16381 ggcgacgccc tcagtcactg gcgaatatct ggccaaggcg gtgtgggatc gtatcgtttc
    16441 ggccattcca gccggaacgc ttcaactcgt caagctcgtc caaactcgcg atctcttcta
    16501 cgaatacgcc ggctgattcc ggtccgtctc ctctcgtctc cctgcaacgt ggctgagcgt
    16561 tgtgcctgcg agggccagcc agccgacgca cgacagaagc tgcacaaact tccaccgctc
    16621 ggcagttctt gccgggtgtt ctgcgtcgag tgctcctcgc actcgcaaat tcactgggtt
    16681 tcaagccgtt cttcatcccg gtatctgtgg cccgaaagtt gtatctatgg atggatgctg
    16741 ggtcgttctg ctcgggtcga tcgggaggta ctgacaccat gatgtccatc tgggaattct
    16801 tcggccaaga cgtggcgctc ttcgggcagc tccatagtcc catgttgagt tggctcggtt
    16861 ccgtcggatt gatcgcgctg tttctatggc atgccgggcg gctgacggcg gccatctcca
    16921 gcgttcaggg atgctacacc cgcgtatggc cgaccttgac gagtctcact gccagccgca
    16981 agagtctgca atcagagtgg ctgacggtcc ccagcttgag cgatgtgaag aaaccggcca
    17041 acgagtccgg tggtcaatcg gagcgggtcg atttggatga tcttcacacg ctcgacaagg
    17101 ccatgcgtcg ggagcctcgc cttgaacagg cctggctgca ttttcgcaag acctttattg
    17161 tggagcgcac cgcctggttc atcgagccga aagtgtttgc gacgcgcacg gcggcagaat
    17221 ttttccctcg tgatctactg aatagccgat tgaatctcgc cttttaccat cagttcccgt
    17281 cgttgattac gggaggcggt ctgttgctca cgtttcttgc gattctcatc ggcctgagca
    17341 aacttcatgc ggatggatca catatcgtgg gcattcaagg gttgatcaac ggattggccg
    17401 ggaaattttt gacgtcgatt gtcgggttgc tctgtgccaa cctctttgtg cttctggaga
    17461 agtccgcctt gcatcggttg ggcaccactc agcagcagtt tgtcgccatg gtcgatgaac
    17521 tgttcccgcg cagaaccatg gaacagatgt tagagaactt cacccccgga gcggggccat
    17581 cacacacgcc tgccgcagtg ggtgcggtgc ccgttgatct tggcgatcgc ctcgtgggta
    17641 ccctcagtga tcggttgagt cccaccgtga cggcgctgag ggaggccgta gaggtgttga
    17701 gcaggcggga accgggtggc cggttcgctg caccggatcg tttggcggag gagttatcgc
    17761 gggtgatgca gcagacgatc gcggctccga ttcaggaatt gaaccaggcg attcacttgc
    17821 ttgcccactc ggtcgaggaa ctcaaacgcg accaaaaaat ggagccagga gcggagccga
    17881 gagagagcga atttgaatcg acgttcttcg atgacacgct gccgtcagaa ttggaagagg
    17941 aagccatccc tgatgacagc atgagcgggt tacggtggtt tgcgaattgg cgtcagggcg
    18001 cctcaatgaa gggcgcggcc tgatatgcgc accaacttct cgtcggattc gcaggaacag
    18061 tcttcgactc tcaccatggg catcacggat ttgatgacgt cactcgccgt catttttatc
    18121 ctcctgttca gcgcctatgt caccaaagtc tccgaaacgg aatcacaggc gaaggtgccg
    18181 gtccccattc cggcgccgga gcggaaggtg gctcggacga cgacggatga tatcaagggg
    18241 ctgttgcgcg accattttca acgattcgac ctttccctgg atgccgatcc caccgatccc
    18301 aatgtggtgc ggattgtcgt gccggaggcg ttgctgaact ttgagttcgg gagaggcgca
    18361 ctctcgtcgg tggcggaccg atttcttgcg gatgcgatgc cgacctatgc ggcgttgctc
    18421 tgcggtgcca tgcgggatca aatcgattcg ctcgtgatcg aagggcatac cgatgatcgc
    18481 ggctcggata tctacaactt gaagttgagc caacagcgtt cgttgaatgt gatggtgaag
    18541 gggttggaag tcatccagga ctcggcgccc tgggcctatc gctgtttcca cgaaaagacg
    18601 tctgccagcg ggcgaggtcg gcaagatctc gtcatggatg agtcgcgtgg ccttgatcga
    18661 gataagagcc gacgagtggt atttaaaatc cgcctgcggc ctacggctca gcagactgag
    18721 atgaagcaag ccatgcggac gattgaagag ccgaacttca gctcccgcct gttctaacgc
    18781 cgttccgggg acggcccgtc ttgcgcgcaa cgaaatccga catcattccg tcgcgcatct
    18841 ggcgcatatc cggcgcgatt tgacgacctg atggccttgg aatcattgtt ccaggcgcct
    18901 ccgcgcaatg cccgaagggg ccctgcggaa ggtcctttcg gattgtctcg tacactgaat
    18961 tggtagtagg tcggatcata ccagtcggcg acccattccc agaggttccc cgacaagtca
    19021 tacaccccct ccggtgtttt gcccagttca aagcgtccga cattgctcag ggtgttgtat
    19081 ccgcgccatc tggtctcacc tgcattggca tgtcccctgg tcggatgcgc acttccccac
    19141 gggtagatcc gtccctcggt gccgcgagca gcaatctccc actccgcttc tgtcggcaat
    19201 cgtttcccca cccatcggca atagtcacgc gcatcgtacc aattgacgcc gaccgccggt
    19261 ttgttctcgt gagcgccggc ggtggcctca ttccacttga acggcgcatc cggtttctgg
    19321 aaggccagga actcggcata acgagagacg gtggtttcgt acacatcaag ataaaaggct
    19381 ttgaccgaga cgcgatggac cggctgttca tcctcctgac cgaggtcgct gcccatggcg
    19441 aattcaccgg caggaatgag caccattggc ccaccacgat gcgagccgat gaacttggtg
    19501 ctaggctcga cgggggcgga gttggatgga ttagctacga ctgaatgtga agggcgtggt
    19561 gaaatgtcct tcgcttcggc agctcctgtg aatgggaggc aatagagcag cgcgatgaca
    19621 caggcgatcg gtacgaatgt ctgcattgtt tcgtgggtga tcgactgcga gacggtcggc
    19681 ggttttggtc tcacctgcac attgtatccc gttcatgcga aaaagtgtgg gggagggacc
    19741 gtgtagggtg cgcgtcctta gcgacggaca agttgcagca gggcttcagt gggtgcggtg
    19801 agggctatcg gagatggagg ctgttacttc agcagatggt cggagattaa tgccgccagt
    19861 tcggccggtt cgaccacccc ttcacgagtc agcagcagtt tgccctgtgc gaagaaatgc
    19921 gtggtggggt aggtctcaaa cgtatgcgct ttcttgatct ctcggcatcg gcctggcacg
    19981 tgcattttgg ccttgccgat cttgatcgtg gggaactttg cagcggtttc ttccaggatg
    20041 ggatcgtatt gcttgcacgg ttcgcaggtg gccaagccgt aggcgacgac tgcgccgaca
    20101 ctctcggtaa actctttgta attttcgtcg cgaacgtctt ggactgtgcc ggtcatactc
    20161 tctcctcggc tgtgagggat gaccgagggg tgggcgtcgt ggtgacctga ccccttcccc
    20221 ctcggtccct cgtggcgctt agctcagttt cgcgagggcc gcgagaatgt ccttatcttc
    20281 ccgtgccacc ttgatttcct gaactttcac gtaggcgact ttgccgttct tatcgacgat
    20341 gaccgttgcg cgcttgccgc aattcaacgg ttcaaaatac agtccatact tcttcacgac
    20401 ttcgcgattg aagtcagaga gcaggcgatg cttcaagtcg agggaatccg cccaggcctt
    20461 gtgcgagaag aagctgtcgc agctgatgcc gaacagttcg gcgtgggcgc tctggaactt
    20521 cgggaagtca tcggtcaggc acttgttttc accctgacag accgggctcc agtcgagcgg
    20581 gtagaacgcg agcacgacat tgctcttccc tctgaaatcg ctcaacttca cgtccttctg
    20641 gtcctgatct tttaatgtga aatccggcgc ggtatcgccg accttaattt ctgcagccac
    20701 atcactcatt gagaatcctc ctaggttaga cctacagcac tacgcaaaac ctttttgtgg
    20761 taccacgcct ggaaaaacct tgtcaacggg gccagaaggc ctagggggaa gcgggtgcag
    20821 tcacaatcac ttgatttcat gatgggttgc ggactttctg aggtggcggg tcagaggatg
    20881 tcagaagtga tttgtcggaa tgccatcatg cggccggtcg gtgctgcggt gcgatgtttg
    20941 atgaatcgga tggtgtgagc gagggccgga aggtccaagg agcttcctac tttttgattc
    21001 tgtccggacc gtaagactgt gtcggccgcg gactttagga gggcgatggt ctcggcgtcc
    21061 ggaaggcctc ggggctgaat cacttccagc tcgtccagtc cgaacttgtt taatcccaat
    21121 gtatggaacc attcggatga ttcatcggta ctctcgctct gctggacggt gacatggtcg
    21181 cggggtaaaa agacattcaa tgatctgtca ttccaatccg acggatttag gtactcgtgg
    21241 cacaggacat cgaaggccgt gccctgggtg agcagtgtca ggccgcgggc gagccgggca
    21301 gcgacgagca ccgtatctgc aatggtgttt ggggcggtgg cggctggtga gatggttccg
    21361 agctgccggt gatcccagga taggacgggc tgccattgtg cggcgtcgga ttgagggagg
    21421 ctggtcagga gatatgcctg ccatggaccg tggctggcgc tgatgcggcc tccggcttca
    21481 tgttcgaggc aggtcagcgg cccgccgtac tgaaggtcgt accagatctt cagttcgtcg
    21541 agagacggcg gcgccccgcg atagccgacc aagtagagtg gagacttcgc gggcgcaggt
    21601 ttcggggatt ttttcctgat cttcatgccg cccgctcagc aaggcgccga gcgcctgcgg
    21661 agttacttgg agctcttctt cgctttccga cggtcatccg ggttcaggag ccgcttgcgg
    21721 aggcggagat atttcggcgt cacctcgacc aattcatcct ggccgatgta ttccatcgcg
    21781 aattccaatg acatttcacg cggtggtgtg agcactaagg cctcgtccgt gccggctgcg
    21841 cgcatgttgc tcaattgttt ctgcttgcac acgttgacgt ccaggtcctc gtcgcggctg
    21901 ttctggccga cgaccatgcc ctggtagact tcgaccgagg cgccgataaa caattcacca
    21961 cgttcttgag tcatgaagag cgcataggcc gtgctggttc cggcttcgaa ggctaccagc
    22021 gacccatgcg gtgccacgag aagcgccttt tcatccgccg gggcataacc ggaaaacacg
    22081 tggtgcagaa tgatggtacc gcgggtcttc gccagcagga tgtttttcag gccgataatg
    22141 ccgcgcgtcg ggatgtgata ctcaatgtgc atttcgctgg cggtgccttc ggaatgcacg
    22201 agcttcatgt gacggagttc gccgcgtcgt ttgccgattt cttcaatcac cggcccctga
    22261 tattcagaag gaacctgaat ggtcagctct tcaaagggtt ccgtgactgt atcgccttct
    22321 cggtgcagga tgacctcggg ctgagagatc tgcagctcat atccttcacg acgcatctgt
    22381 tcgatcagca cgccgagatg aagttcgccg cggccggcca cgagaaaccg gtcggcgctg
    22441 tctgtttcct gcacgcgcag ggagacgttg gtttccagct ccttgaacaa tcgttcacgc
    22501 aggtggcgcg aggtcaggta ttttccgtca cggccggcga acgggctgtt gttgaccgaa
    22561 aacgtcatct gcacggtcgg ttcgtcgatc gtcacgcgcg ggagcgcgac cggattggtc
    22621 ggatcggcaa tcgtgtcacc gatgttcact tcttccaggc cggagagagc gacaatttca
    22681 cccgcttcag ccgtttcgac atcggtgcgc tcaaggcctg aaaacaccgc aagatcggaa
    22741 atcttgccgg ggacctggcc gccgtccttg gtcagagtga ccacgttttg gcggcgggcg
    22801 atggagccgg attggatctt tccgattccc attttccctt tataggaatc ctgggccagg
    22861 gccagcacga ggagctggaa cggggcgtcc gcgttgatgg ctggtgccgg aatcttgtcg
    22921 agaatcgtct ccaggagcgg ggagatctcc gtccctggct gcttgagatc gagggtcgcc
    22981 acccccttga tggcggaggt atagacgata ggaaagtcca gctgttcgtc ggtggctccc
    23041 agatgcacga agagatcgaa ggtgcgattg acgacgtcat cgatgaccgc atcggggcga
    23101 tcgattttgt tgatgacgac gatggcttta tgtccgaggg ccaacgcttt gcgcaagacg
    23161 aacgtggtct gcggcatggg gccttctttg gcatcgacga ggatcagcac gccatccacc
    23221 atgcggagtg tccgctccac ttcgccgccg aaatctgcgt ggcccggcgt gtcgacgatg
    23281 ttaatcttca cacctttata ggtcacgctg gcgttttttg cgcggatcgt gatgccacgc
    23341 tcgcgctcct gatccattga gtccatgatg cgctcaccca tgtcatcgat cttgcgatgc
    23401 acgtgggtct ggcgcagcac ggcgtcgacg agagtcgtct ttccatggtc gacgtgggcg
    23461 atgatggcaa tgttgcggat gtcggtccga cgaccttgcg gcgcgtgcag acccgatgtg
    23521 gtggatgagg gcgctgagct gttcataatc tccaatgccg tgaaagtgcc aaaaaaaaga
    23581 cgccttccta tgaaggcgtc taagcctatg gactataccc gattcaaatg tcttcccgca
    23641 agctgattgt cgaatgttcc gcggaaactt cttctgacga tgattcgaac cttgcttgag
    23701 tttgtcgggt gtgcattgta tggtggggtt gtcgcgggtt gaccccttct cctcgcaacc
    23761 atccaacttc atgtccgaac tcgatcactt actcgatacg ccggagaagc ctcgccgtcg
    23821 ctggcgatgg tggaagattg ccgtcatcgg cctgctgctg gccattgtgc tcggtggcgg
    23881 cggagcggcc ggcattctct ggtatttttc tcaagacctg ccctcgctgg atcccttgca
    23941 gaactatcag ccgagcctgg tgacgcgcgt ctattccgac gaccggcaag tgattggcca
    24001 gtttttcatc gaacgacggt tcctcaagcc tattcaggag atgccgaaga gcctgaccca
    24061 agccgtcatc gccacggagg acacgagatt ttttgagcac ccgggcttgg acatcgtcgg
    24121 gattctccga gcggcctgga ccaatattcg ccatggcggc cggaaagtcg aaggcgccag
    24181 taccattacc cagcagctgg cgcggtccct gtttctttcg gcagagcgga cgttcgatcg
    24241 caaagtgcgg gagctcattc tggcctacaa gatggagcta gtgctgacga aagaacagat
    24301 tctggagatg tatttgaacc agatctattt cggccagggc gcctatggcg tggcggcggc
    24361 cagccagacc tatttcggga aagaactgtc aaaacttacg ctggctgaat cagcgttcct
    24421 cgccggcctc cccaagtccc cgagtcacta ttcgcccttc aaggcctatg accgggctaa
    24481 gaagcggcag gagcatgtgt tgagccgcat ggcggaggca ggtttcatca atgccgccga
    24541 tcgcgaacaa gccgtagccg aaaagctgaa cttccgccgt cctggcagcg aacatctcgg
    24601 gccctatttc gtggagtata tccggcaatt gctggtggcc aagttcggtg aagcgatggt
    24661 gtacaagggc ggattggaag tgttcaccac gttgaatgcc gatatgcaga aagcggctga
    24721 aaccgctgtg tttcatggat tgcgcgaggt cgataagcgt cagggctggc gcggtccgct
    24781 ccgcaccgtc gatccgacga ctttggaagt gccggcaccc gatccctcgg tgaagcttgc
    24841 ggagggggat atcatggaag gggtggtgac gaagatcgcc aaagaccacg tgctggtcca
    24901 aatcggcggt agcaccggtc ggctggcctt cgacgatatg gcctgggcaa ccaagtatct
    24961 caagggcaag gatcccacga aggatgcgac tccgatcaaa aacctcaagc aactgttggc
    25021 actcggtgat gtgatcgagg tcggagtgaa gaagcttgag aaggagacgg tgcatctgcg
    25081 attggaacag actccgttgg tcgaaggcgc gttggtggcc gtcgatccca aaacaggcgc
    25141 gatccgcgcc atggtcggcg ggtatgattt tgcgcggagc gaatacaatc gtgcggtgct
    25201 cgcgcgccgt cagcccgggt cctcgttcaa gccgatcatc tacgccacgg cgatgaacca
    25261 aggtatgagt ccggctacgg tggtgctgga tgccccggtg gtgtacgaac aggaggaaga
    25321 agataagacg tggaagcctg aaaactacgg caagcggttc cacggcattg tgagtttgcg
    25381 ggaagcgctg attcattctc acaatctcgc gaccgtgcgt ctcctggata aagtgggcat
    25441 tcgcaacgtg atcgatttct cgcggacggt cggtattgtg agtccgctgg ctgccgatct
    25501 ttcgctggcg ttgggttcgt ccggcgtggg gctcatggag ttagtgtcag tgtacggtgt
    25561 gtttgccaac cagggcatgc gggtggagcc ttatgcggtg gcaagcgtgc aggacagtgg
    25621 cggtcgcacg ttggatcagg cggccattca gccgcgtcag gtcgtgtccc gcgaaacggc
    25681 atacctcatt acgaatatga tggaagatgt tattcaacgt ggaaccggtg tggcggccaa
    25741 ggctgtcatt gaccggccgg tggcgggaaa aaccgggacg accaatgact tcaccgatgc
    25801 ctggttcatc ggttcgacgc ccaatctggc gaccggggcc tgggtgggtt tcgatgatcg
    25861 tcgcccctta ggtgaaacgg aatccggcgc gcatgcggcc ttaccgatct ggatcgcgtt
    25921 catgaaagat gcgctcaagc aactgccggt tgtgccgttt gaaattccag acggggtgat
    25981 gttcgtgaag gtggatccca cgacggctct cctcactgat caggacgagc agcacggcac
    26041 cgtggagctc tttaccaaag ggactgagcc aaccaagagt gcgggatcca agatcgatcc
    26101 caccgagttc tataagctgg accaattgca ggacagtgcc ccaccagttt ctgtcgagcc
    26161 gtagcccgac gccgggcaag tttcctgccc cagtcttctt ctctcattct ggcacagcgc
    26221 tgtgacggtc gtcgctctgg cgccggcctt gctgtgagat gtgtggtgaa ggacgttcgg
    26281 ttggccgatc aggactggga atcctgatat tcttcgtgcc aggccatttg aatggattcg
    26341 agaatttttt cgttcgactt cttcgggtcg tccttaaatt ctgggagggc gacgatccat
    26401 gtatgcatgt cggtgaagcg gacggtgagg ggatcggtgg tcgggtgggc ctctaccaaa
    26461 cgaatggcga gttcttcagc gtcactccac ttcaaatcca ttgcagttcc tcgtgttcag
    26521 gattgcgtca cgtcagagac ttttttgcct tggagccctt gcttcaggga atcgtccagc
    26581 atggccacgg tgaggccttt ggctgcctgc tcaatgtcag ccatgcgtgc gcggatggca
    26641 cggggatcgc ttccgtcctt ggcggtcgcc agggccgaga gggcggtgcg aatggcctcg
    26701 acgtcccctg tggcgacgag atgcccgcca tcggccaacg atttttcggt cgtggtgatc
    26761 agggctcccg catcgagtcg ggcttcgatc aattttcgcg cgttgacatc gtctgcggcg
    26821 aacttgaacg aatcttcgat catccgttcg acttcttgat ccgataatcc gtatgacggt
    26881 ttgacctcga tcgattgggt ctctccggtc cgcatgtctt tggccatgac attcaggatg
    26941 ccattggcat cgatcaagaa ggtcacttca atgcgtggaa ctcccgcggg caatggcggc
    27001 accttgagac ggaaacgggc caggctgcgg ttatcctttg ccagctctcg ttcgccctgg
    27061 agaatatgga tgtccacccc ggtttggccg tccacatagg tggtgaacat ttccttggcg
    27121 ctggcgggga tggtggtgtt tcgtctgatc agactgctca tgaccccgcc cattgtctcg
    27181 atacccaatg aaaggggggt cacatccaac agcaacatat cggtcgtgcc gccgctcaga
    27241 atatcggcct gcaccgcagc gccgagcgca accacttcgt ccggattcag ctcgcagtgc
    27301 ggtcgtttcc cgaagagggc ctgcactcgt tggcgaacca gcggcatgcg tgtcgagccg
    27361 ccgacgagga cgacttcatc gatgtcgccc ggagttaacc ccgaatcctt caaggccagg
    27421 cggcagggac ccagcgtacg ctcaaccaat gcggtacaga gcccttcaag ttgatcgcgt
    27481 gtgagttcgc gtgtaaaccg gcccttactt tccgggaggt cgagactcac ggcggtcttg
    27541 agttcttctg aaaggcgaat ctttgcgcgc tcggcttcca ggcggactgc ctgcatatgg
    27601 tcggaatagg tgctgagatc gagtccatac tgcgtgcgga tgtcagcgag gaacaggtcg
    27661 gcaatcaact ggtcgaagtc gtcgccgccg agatgcgtat cgccatttgt ggcgagcact
    27721 tcaaagatcc cgttttttaa tttcaggatc gagatatcga acgttccacc gcccaggtcg
    27781 tagacggcga tggtgccctg cgtcttttct tgcaacccgt aggccagtga ggcagcggtt
    27841 ggttcattga tgatgcgcaa gacttcaagt ccggcaatca tgcctgcgtc tttggtcgcc
    27901 tgccgctggc tatcgttaaa ataggcaggc acggtaatga cggctttggt gatgctttct
    27961 cccagaaagg cttcagcacg gagcttgagt tctttcagga tcatggcaga gatctggggt
    28021 ggggagtagg atttctctcc cagcttgatc cggatgacgc cacctttttc tgtgagcgtg
    28081 tagggaaagt aggccagttc gttttggacg tcggccaggc tcttgcccat gaaccgtttg
    28141 acggagtaga ccgtgcgctc gggatttctg gtgagatgtt ctttggccgg gtcgccgaca
    28201 atgagtccgt tgtcggtcag cgcgacgaca gaggggacca tcgcgcgttc gtggcgtgcc
    28261 gagatgacgc gtggcgtgcc atgatccatg taagcgacaa gggaattggt cgtgccgagg
    28321 tcgatgccga caatgcgtgc cataatgatt aggcgatcgt tgcgacgaga tcgttgacga
    28381 tgttgttcac gtaggtgcgg ttggacagga tgtcccgcat ttccttgaga atgcggccgc
    28441 gctcagcgcg tgcctgctcc gtggcctcgc cacgatcctg cagtgcatcc caccggctga
    28501 aaagctgttg gagttgggct tccatatccc gttgccgttg ctcaagtgtg gcgcgatcag
    28561 cgtggagttt cgtgcggagg gtagacgcac tttcggaggc gcgatcactg gcgcggaact
    28621 catcgagggt atcctgcaac tcgagaattt cttcgaagag atcggccggc ggtgtcgtcc
    28681 ggatatcttt cacggagccc gcttcgaggt ccaataggta ttcggctcgt tgaatcgggt
    28741 ctcgtagggt gcgataggcg gtgttcagca tggccgagtt gccgaggctg atggtctgct
    28801 ccgcatcgct tttattctgg tagaagtcag gatggaatgc ccggctcaac tcgtagaact
    28861 tggtttccag tgtctgcgga tcaatcgcga ggcgccgtgg cagcccaaag caggtgaaat
    28921 agtccagatc cttggagacc ggttggactt tcacgcaacg gtcgcaaaag tactctccag
    28981 ccatttcgga ctggcaatgc cagcacatgc tgcgagccat ctgcagctcg cgtcgatcat
    29041 cggtgttatg aatatgtccg ctcatgggct gtcgctcgct caggccgaaa aggattcccc
    29101 gcagccgcac gtcttgttgg cgttggggtt cacgaatttg aagtttccgc ccatcaggtc
    29161 cttctgaaaa tcaagctggg tgccctgcag gtaaatggcg ctcttggcat ccacaatgac
    29221 cttgacgccg tcgaattcgt acacttgatc gtactggccg atcttttcgt cgaaattgat
    29281 ggtgtagctc aggccggagc aaccgcctcc cttgacgcct aagcggaggc cgccttcttc
    29341 gatgccttgg acattgatga gacgtttgac ctctttcagg gctgcgtcgc tcagagtgat
    29401 gaccggggct tgagcatcgg tattcgtggt ggcgtccata gtgcgctcct tcctgtcgtt
    29461 acttcgagtc ggctttcttt tgatagtctg cgagggccgc tttgatggca tcttcagcca
    29521 gcacggagca gtgaatcttg acggggggca gattcaactc ctgcacgata tcggtgttct
    29581 tgatcttgcc ggcttcctcc accgttttcc ccttgagcca ttcggtggcc agactggagc
    29641 tggcaatggc ggagccgcaa ccgaaggtct tgaacttggc gtccacgatc gtgtcgtttt
    29701 caactttgat ctggagtttc atgacatcgc cgcattccgg ggcgccgacg ataccggtgc
    29761 cgactccatc ttcttctttt ttgaaactcc ccacgttacg ggggttgttg aagtggtcga
    29821 cgactttttc gctataggcc atggtgtcct ccgaatgtat cgctcacagt agtgaagttt
    29881 caggttaatg tgctgcccat gacacggatt tcaggtcaat gccttccttt gccatttcgt
    29941 acagcggtga catttcccgg agcttggtca cgacttcgat aattctcttt ccggcatagt
    30001 ccacttcttc gtcgagcgtg aaccgtccca ggccgaatct gatggaggaa tgcgcgagtt
    30061 ccgctcccac acccaatgca cgcaacacat aagagggctc cagggttgct gacgtgcagg
    30121 ccgagccgga cgagagagca atttccttgc agcccatcag cagggactcg ccttcgacgt
    30181 aggcaaacga aatattcaga ttgtgcggga ggcgttcggt cggatgaccg ttcaagtaga
    30241 cctcttccag cgccttcgta atggtcgcat ggagccggtc acgcatgacg gagagtcgcg
    30301 cgccatctgc ggacatttcc tgctcgcaca gttcgcaggc ttttccgaac cccacaatca
    30361 agggtaccgg aagagtgccg gatcgcatgc cccgttcatg gccgccccca tccatttgtg
    30421 ctgcaatgcg gacacgagga tttttcttcc tgacatagag ggcgccaacg cccttggggc
    30481 catagatctt atgggcgctg aacgacatca ggtcgatacc catgtcctga acatcgatcg
    30541 ggacttttcc gacgccctga gtcgcgtcgc aatgaaatag aatgcccttt tccttcgcga
    30601 tcttcccgat tgctttgacc gggttgatgg tgccgatctc gttattggcg aacatgacgg
    30661 agatcaagat ggtcttgtcg gtgatggcgt tccgcacgtc ttccggattc accatgccga
    30721 acttatcgac cggcagatag gtgaccttga ccccgcgttt ggcttcaagg gttttggccg
    30781 tgtccaacac cgcgcgatgt tcggtggacg acgtaataat gtggtcgccc ttttcgtgat
    30841 acatctcgac gacgcctttg agcgcgagat tgttcgattc ggtggcgccg ctggtgaaga
    30901 caatttcctt ggcatccgca tggatgagct tggcgatctg ttttcgcgcg gcttcgacac
    30961 cttcttcggc atcccatccg aaggcgtggt tgcgactggc ggcgttcccg aatttttccg
    31021 taaaataggg cagcatcgat tccagcaccc gtggatccat cggggtggtc gaatggttat
    31081 ccaggtagat cgggaacttc atcgctcgac tccttgttgt tccgtcgcga cggattgaat
    31141 ggtgatgagt ggggtgcccc ccatcatatc ttgcaaggtc atattgttga gaagctgata
    31201 aatactgctc tggattttca gcaacggggt ccgaatgtta cagtgctcgc gctgcatgca
    31261 ggcatcgccg tctttttcgt gtgaacagtc catgataccg agtggacctt ccaggctttc
    31321 gagtacgtgg gcaatcgtga tctcacgcgg attcttgccg agcagatagc cgcccttggg
    31381 gccgttatga ctctcaatga ttccgctctt ggagagggtc tggagaactt tggcaagtaa
    31441 ttcgaccgga atgtgatatt cctcggcgat ttcctttgtg tttactacgc gagcttgggc
    31501 gacatcgccg tattggttag aggcgatgtg ctggagtgcc atcaacccgt agtcagcttt
    31561 tttcgataat ttcagcatgg gcctattgcc gatccttcaa gtcggagact ataatgatct
    31621 ccgatcaaat cagtcgtaca aaaggtatca tgtggtcttt gggcctgtca agcgagcgga
    31681 ggggcggaat aagtaagaat gtcgctctct ggaggcttgc gacgagtgat acggaagcag
    31741 cacgatacat tgcaccacat gcatgacaaa aggggctaag caacatgggt gggacaaatc
    31801 cctacattga aaaggcagac gtggagctgc cgcaaaaatc ttatatcgtg accttcattt
    31861 ttcccgacaa aacggaaaag aaggttgagg tacaaccgga caagattccg tacggtccta
    31921 cgggcctgcc gggaagtatt ctcgacattg cgatgggcaa tgggatcgac ctggaacatg
    31981 tgtgcggtgg cgtctgcgcc tgctccacct gccatgtcat ggtgaagaaa ggcttggaga
    32041 cgtgcaatga ggggacggat gacgagttcg accagttgga cgaagcgccc gtcacgacgt
    32101 tgcagtcccg actcggctgc caatgtgtgc cgaatggaac ggtggaagtc gtggtagaaa
    32161 ttcctgccat caacaagaat ctggcgaaag aagggcactg aaggcaaaga gatggagtgc
    32221 aggtcgtata gcggtcgtat cagtcgtacg tttccagctt cacttcccgc cgatcgaacc
    32281 ccagttccat caacagtgcc cgcaatgggc gtgcaaatgc tttgaccccg gcaatcatgg
    32341 gggtcacccg ccgaccgttt cccgcgattt gtttgatcac atccacagtc tcggcattgc
    32401 tctctgggcc ttcgttgaca aacggcagat accggaaatt ggattggctt gccgccaggg
    32461 ccatgaattc ctcatggtag agctgttcgg cagtggtggg tgcttgggcg atcagcgtgc
    32521 ttgaaggtag cgagccctgg cttgccagtg cgcggagcat acagcgcagt gggacgagcc
    32581 cagaatagcg tccgatcaac agcaagtgct gaggttccga gtcgggcagc acaaagtggc
    32641 cataggggcc tgacagggga atgcgattcc ccggcttgag cgacgcgaga tagccggatc
    32701 ctttcccgcc tggaacgtgg tcgaagagca gtgtgaggtg gccggcggta gacggcggtt
    32761 ccgccaacga gtaggcacga ttcaatggcg gatggtctcc gaccggcaat tgcagtgaga
    32821 tccactgccc gggcttgaag gacagcgggt gctcgatcgg gcggagcacg agttccgtcg
    32881 tatgcggagt cagggtcgtg agcgagatga tttcggcaat ctgtgttggt tcggccatgg
    32941 tctgttctta acaaaaacgg attcagaatg gaacaggcca gttctgtaca tcattccaac
    33001 gccatgttat agatagcgcc ccgtttacta ttccacacga actacggtga tgcgatgagt
    33061 caggtcaggg ttcgatttgc gcccagtccg acaggttttc tgcatatcgg cggggtacgg
    33121 acggccttgt tcaactggct gtttgcgcgg cagcagaacg gggtctttgt ccttcgcatt
    33181 gaagacaccg atcaaagccg ttctaccgat gactccattc aagccattct tgacggcatg
    33241 cgctgggtca aactggattg ggacgaaggg ccctttcggc agaccgagcg aatggatctc
    33301 tatcgtgaac acgcgatgcg attattcgag caaggccatg cctattggtg cgtctgctcc
    33361 tcggaagagc tggaggcgcg tcgaaaagag gccgaaacca aaggcggatc gcccaagtat
    33421 gacggccggt gtcggaatct cggtctgaca aaaccgaccg gggaagcggc gctccgcttt
    33481 agggctccgc aggacgggca gacggttgtc gatgatttga tcaagggccc tgtggtgttc
    33541 gacaaccaga ttctggatga tgtcatcatt ctcagatcca atgggtatcc tacctataat
    33601 ttctccgtcg tcgtggacga tgccttgatg gggattaccc atgtggtgcg gggcgatgac
    33661 catctcacga atacgccgcg ccagattcca atttttcagg cgctgggatt tccgttgccg
    33721 cgattcgggc atttaccgat gattcttgga ccggacaagg caaggttgtc gaaacggcac
    33781 ggcgccacct cgatcatggc ctacaaggac atggggtatc tccccgacgc gatggtcaat
    33841 tacctggttc gcttaggctg gtcgcatggc gatcaggagc tgttctcgcg cgcagaactg
    33901 atcgagaagt tctcctggaa gaatgtgcag acctcggcgg ctgtgtttaa tccggacaag
    33961 ttgctgtggc tcaatgctga atacctcaag accagccctc cggctgagat tgctcaggcg
    34021 ctcgtgccgc ttcttgagca ggccggactc aaagaggagg ttcgtgccgt gtcgaccgaa
    34081 tggctcgcgc agctggtcgt cttggtgaag gaacgaacga agacattggt ggagatggtc
    34141 cactgggtga caccctactt cggccaggcg gtcgtgtgtg acgaagaggc ggccaagaaa
    34201 ttcctcacgg ccactcatgc gccggtgtta gccaaactgc tgagccgatt cgaagccttt
    34261 cccacgtttt ccaagcagga gtgggaagag tcatttaagc aactggtcga ggaagagggg
    34321 atcaagatgg ggcaactggc ccagccggtg cgggtggccc tgacgggacg gacggcaagc
    34381 cccggtctct tcgaggtgat ggaggtgcta ggccgcgatc gcaccttgct ccgacttcgc
    34441 aaggggatcg agcaggcctc cagatcctga gaatcggccg gtcaagaatc agcatcgatc
    34501 ttgacgcagt agagaagctt atattactgt gtggaccggt tgggggatcg tctagcggta
    34561 ggacgtcagt ctctggatct gactacctag gttcgattcc tagtccccca gccaannnnn
    34621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctacc taggttcgat tcctagtccc
    34741 ccagccaatt tccctcccag acgatcctct gagaacgtac gcgactcagg tcgagatgtg
    34801 aggctgtcag cgccacaact ttgaacgggc ggtcgttcct tcacgaactc tgcacggtct
    34861 gtgcacgttt ttcatccagt tcggcccaac gggcatagag ccgctccacc tcagcttgtg
    34921 ccgccaccaa tgcctggctt cgtgcctgga gcgctgcggc atccgacagc acggccggat
    34981 cctgcatcgc ggcttggcag gctgtgacct cttcctcggc tttgaggata ctctcttcga
    35041 tcgtgcccca ttccttctgt tcgcgataac tcatcttttt gggtttgcga gcgggacttg
    35101 tcgaggcagt ctcgtcctgg accgcagcac gcccgtcact cttcgactcc ggggcggatc
    35161 caccggcccg ctcctgcgca ctttcccact gggcatagtc ggcaaaccag tccacgcgtc
    35221 ccgtgccatc cagtgccagc aggatggtcg agacgcgatc cagcagccac cgatcatggg
    35281 tcaccagcac gagcgcaccg gggaattcca gcaggctatc ctccagcact tcgagggttg
    35341 gaatatccaa gtcattggtc ggctcgtcca ggataagcag gtccgcaggc tggagcatga
    35401 gtcgggcgat gaggagccgt gcctgttcgc cgccggacag tttcgagacc gggagatcca
    35461 gttgttcggg acggaagaga aagcgtttcg cccatgaagc caggtgaatc gagcgatcct
    35521 gatagacgac ggcatcgccc gacggggcca gtgcgcgtcg caagctcgtc gattggtcca
    35581 gggactcgcg atgttgctca aaggagacga tacgcagcgc gtcggctcgc gtgatggtgc
    35641 cggaatcggg ctccagcgtc ccggccagta accgcagcag ggtggattta ccgctgccgt
    35701 tcggaccaag gagtcccagc cgttggccgg gcccgaggag caggtcgaga ttggtgatga
    35761 cgggcgtccc gttgaagctc ttgctgacct gctccgcgac cacgagttgt ttggactttc
    35821 ggcccgaggc ggagaagtcg atgccgatgg tcgactgtgc cgaacgggat tcggcctggt
    35881 tcaattcgtc gatgagcttg cccgctgatt ggatccgacc tttggctttg gtcgtgcggg
    35941 ccttggggcc gcgccgcagc cactccactt cacggcggac gcgattggct aatgaggcct
    36001 ggtagtcggc ttgtgcttga agggcggcat cgcgctgttc gaggaaatca ctatagcagc
    36061 cgcgggattc aaaccggcct tcgggataac agcggttgag ttccaccatg cgagatgtca
    36121 ccgattcgag gaaccggcga tcgtggctga tcacgagaaa ggctttggcg cgattcttca
    36181 gcagttgctc cagccagata atgccttcca agtccaaatg gttcgtcggc tcgtccatga
    36241 gtagcacatc tggctcgagt agcaaggcct gggtgatggc caatcgtttg cgccatcctc
    36301 cggagagtgt ggaaacggcc tgttccgggt ctggaaaggt tccgatgctg agggccctgg
    36361 cgatccggcc accctgttcg tgaggatcca gcccgtcttc cgtcaaggtg tcttgcagca
    36421 cctgttcaat gctgtgatgc ggaggaaaca ggggttcctg cggaacatag ccgatgcgtg
    36481 tgtggcgccg cagggtgcgt gtgccttcat ccggggattc gagccctaca aggatcttaa
    36541 gcagggtcga tttgccggat ccgttcggtc cgattaatcc cacatgatcg ccgtcggaga
    36601 gtgccaggga gagatctgta aacagggctc tgaccccata gcttttgctg atcgattcgc
    36661 aactcaacag aatgttcggg gccataccct gggtacctct ttcgtcacgt tgaaagcatg
    36721 cagtatacgg tgtggtgggg gctcggggct agcggctagc ggcgcgggag ggagtgctct
    36781 accgtgccgc cgagtgaaac gatgtgaggt atcttgcggg tgggaatgta aaaatcagtg
    36841 cgccgttcga gcgcgtcccg tgcggagacg ttgctgaaag cagctcgggc agagatggta
    36901 accaccctgt gtggaggtat gaaatgattc cattttggtg gtcccgcaat caaaacagcg
    36961 cacctcgctc accacagacc gtggcaaggg gggcgtggga tcaatgcgct gcagttggtc
    37021 gtgcagacgg cgtgtggagc ggtagatgtg ccggaggagg ggttgtgctt cggttgccgc
    37081 attggggatc gcggcttcga ggagggagag gcgttcgcga aacgagcccg gttcttcgag
    37141 aggctcttca ctgcaaggtc ccgtcaccat cgttgaggct cctttgtcgt ggagtgatgc
    37201 ccgatcagac gagtggcgat tctcgttcat cggcgtgaca gggttcaagg gtgccgaagg
    37261 ctggagtccc cgtactttgt gagggacgat ggaatgggag gattcgatgg gacttggtgc
    37321 tggtcagcga ggggtttgag ctttctcgat cttggcccaa gtgtctttga gtgtgacggt
    37381 tcgattaaac accaacttgt cgggtgacga atcggggtcg gcgcaaaagt atcccacgcg
    37441 ttcaaattgc actctggttc cgggggcgat cgcagccagg ctgggctcta ccttgcaagt
    37501 cggcagtcgc tccagcgaat ggggattcag gtgctgggtc cagtcctggt cagcgggaat
    37561 cttgcctgga tccatgagca tgaggctctc gtatagccga atttctgcat cgatggcatg
    37621 agcggccgat acccaatgga tcgtcgcctt caccttccgt tgcgcctgcg cgccgccgct
    37681 cttggtttca gggtcatagg tgcagcgcag ctccgtaacc tcgcccgtcg ccggatcttt
    37741 caccacgccg acacaggtga tgatgtaggc atacctcagg cgcacctcgc gacccggaga
    37801 aaggcgaaag aactgcttcg gcggatcttc tcggaaatct tcctgatcga tatagagggt
    37861 gcgtgagaag ggcacctgac gcgtcccggc tgaagggtct tccgggttgt tgacggcttc
    37921 gagttgctcg acctgtccct ccgggtaatt ctcaatgacc agccgcaagg gccgaagcac
    37981 cgccatcaca cggggcgcac gtttgtttaa gtcttcccgg acgaagtgtt caagcagttg
    38041 catctcgacc acggcatccc gtttcgcgac gccgatagcc tcgcaaaagg cccgcagggc
    38101 ctcgggtgtg aaaccgcggc ggcggaggcc tttcagtgtg ggaaggcgag gatcatccca
    38161 accgttcacc agcttgcgtt ccaccagatc gagcaatttt cgtttgctca tgacggtgaa
    38221 ggtgacgttc agtcgggcga attcgatctg ctgcggacgc tgtgcagtgc cgcagtgctc
    38281 gaccacccag tcgtacagcg gccggtggtc ctcaaattcc agcgtgcaga tcgaatgggt
    38341 gatgccttcc atcgcgtcgg agagggggtg cgcaaagtcg taggccgggt agatacacca
    38401 ggctgtgccg gtgcggtaat gggccacgtg tctgatccgg tagagcacgg gatcgcggag
    38461 attgatgttc ggcgaagcca tgtcgatctt cgcccgcagc acgtgggtcc cgtcgggaaa
    38521 ttcgccggca cgcatccgcc gaaacagatc taggttttct tcgatgctcc gggtgcggaa
    38581 cggactggct tgtcccggct cggtcagggt tccgcggtat ttgcgcatgt cgtcggccgt
    38641 gaggctgtcg acgtacgcga gacctttctg aatcaggccc acggcatagt cgtagagccg
    38701 ctcgaagtaa tcggatgcga aaaacatctt gtcctgccag tcgaagccga gccattggac
    38761 atcgtcttga atggcctgca catattcagg atcttccgtg gtcgggttgg tatcatccat
    38821 gcggagatgg cagatgccgc cggaattttc gttggcgatg ccgaagttga gacaaatcga
    38881 tttggcatgg ccgatatgga gatacccgtt cggctccggc ggaaagcggg tgaccactcg
    38941 gccttcatgt ttgccggcag cacgatcggc tgcgacgatc tcgcggatga agtctgaacg
    39001 tgtcggtggt tctggcgtgg tggacataat ggttgcgagt ccgtcgagtt gcaggtgatg
    39061 aaacgcgcat ggttctatca cagaacggcg gggtgggaga agagggcccg gcgtctcagg
    39121 gcgtggcgag tcgattttcc ctggatcgat cggaaggact attttagaat caggctgctg
    39181 tggaaggcgg atgtctgacg ggcgccgagt aatggagaag aagagatgat gaatggtcga
    39241 tggagcgcat gcggactggt ggtgctgctc ggcatcctct gggcgggcgg aagccaggct
    39301 gtggagccgg actcactcga ggcgtgccgg gcccaaccgt tcgacgccaa gatgcagtgt
    39361 tatcaacggc acttggaaac agtccttcag gcagagggca cagaacaaac gctcacggtc
    39421 ttggagcaga tcacggttca ggatcctgag gcattgcgtg aggcgcatcc gctggttcat
    39481 cacgtcggcc agcggtcgtt tgcgcgctat ggtacggcac cactcgcgtt ggcccattgt
    39541 cgagacgtgt tttggtctgg ctgctaccac ggggtcttgc aggccttcct cagcagcctt
    39601 gcgactgtcg agccacagca tattctgccc ctgtgcccga tcagcgagac ggtctccgcc
    39661 tattcgtttc agcggtacaa ctgtctccat gggttgggcc atggcctgac cattcaattt
    39721 cgctatgacc tactcaagag cctggccttt tgtgatgcgt tgccgggcag ttgggatcgg
    39781 gagtcctgct atggcggcgt cttcatggaa aacatcgtga cgttccagca ggcacaacgg
    39841 gcgcagccgg cgcaaacggg cagcgaacat catcaccatg aggccacgag ttttctcaac
    39901 cctcaagacc tcctctatcc ctgctccgtc ttgaatgaga aatacctgcg agcctgttat
    39961 ctgatgcaga cctccgccgt gctgaccttt ctgaattatg attttgccca ggccttcatg
    40021 cagtgcgcca gagtggaggg ggagcatcag acgacctgtt accgcagttt gggccgtgac
    40081 atcagcggct acacgttgcg tgatgcggtg cgggtcaacg aactgtgccg gctggggcag
    40141 gccgatcaaa tccggcaatg tttcatcggc gccgtgaaag acttcattct caccgacgcc
    40201 aatccggacc cagggctcgc gctgtgccga agcctggatg ggccgttcaa aaaagactgt
    40261 tatgccaccg tgggtgaaat ggtgatccct ctcttcgacg acaaggagaa gcgggcgcaa
    40321 gcctgccgca aaggcgaaga cgagtatgtt gatgcctgcc ttgcagtcgc cacggccttt
    40381 tagcgagggt ctttcggaac tcccggtcca gtcacgccgc tcggatcctg cccctgatca
    40441 gaaccgtatc tcgtaaagcg cgaagcgcat ctcggtgagg ataagcgcgt atggctcatg
    40501 gcttatcgct ggtagctacg cacctcactt tccagttcag accacacgct cttttcttcg
    40561 acgagatacg aacgacgctt cacggtcgcc gaaggagtcg cggggaatcc gttcgaggtc
    40621 aggtgccggg cggggcagac acctggacta catgaaaatc cttttgcgcg atcatacggc
    40681 cgttcatgcg gagacgaaac tcataacgac cgggcgcggg gaagagcagg gccgggatgt
    40741 tgataccgaa gtcggaaatc tgcagacgat ctccaatggc gatatcgggg agcgatgcgc
    40801 ggcagaccaa ctggtctgta ttgacgaaca tcagatcgat ctcgacgtga tacgtgcctt
    40861 cggcgtcggt gatgcagaag tacaggccga gctgatggtg ctgaaacggg aacgtcaccg
    40921 actggaggtg ggagaacagg ccgatcagag attttttctt agtcaggcta tcttcgatga
    40981 cgcagtcgca gacgaggaac gcctgcacgc tgggcggcga gacgtcagtc atgcgatccg
    41041 gccattgtca gccgagcacc gcggtgccgc cgcgggcctc gaagacatcc ccgaacagcg
    41101 tatgggtgga tccgatgcga cagaagtgcg acgtgatctc gatgagctgg ccgcggtgcg
    41161 aatagaattt cccggtcgcc acgtggattt tttcacctgg tgcgacagcg gcttcaagga
    41221 ccgtttcgcc ggtttcctgg tccgtgactt tgagccatgg cagattggga aagagggtcg
    41281 gcggggactc gctggtgttg agtgaaaagc gattgccgtc gttgaacgcc cagcgattgc
    41341 gacgtagatg ggccacatga ttgccgctgc cgtcatagag gtcgatggtc agcaggattc
    41401 gctcctgatc cggcgcaagc tccagcacga gctgctcttt gccttgaacc cggatgactc
    41461 cattggtatt ccggaaaatg ttggaaccga gccggatttc caggctgtgc ttgtgccgat
    41521 ccgattcggc ttcgtgggag gagtgtgctg ggtcaggttt gcgtagttcc atgcgtagag
    41581 atctagggcg acccgaggac ctgttgagcg acgcactcga ccatacgttc ttgcctgagc
    41641 aagtcgggag caaggtaggc ccttggcggg gcgctgtcaa gtccgttctg gtcgggctag
    41701 ggagaggcgg ggccttcgcg ctcgtcggaa tcggtggcgg tcacacagac ggtgacttcc
    41761 tgttccgatt ggtcacgcag cggaatcatg acgacttggg acacagccgc tccgccggtc
    41821 tttccagacg cggggacgac cttgccgatg accgggccat ccccggcgtt atagtaaatg
    41881 gtggtatagg caaggttggt caagggtttg ccgtcggtgc cggtcgtggg ttcggtgtag
    41941 gcgaccgtga cggtcgaatg tggcggcttc acgcggacct tggtggtggc cccgcatttg
    42001 gtccaagcgc ttgaataagg gggcgcgatg gttccttcca gaaagctcgc cccgcagccg
    42061 gccagtgaga ggaaggccgg aagcagaccc gcgatcaaaa ccgtttggag gcgagtggag
    42121 cgtagaacca tagtcctact ttaggccaat tcaataggct ccgcaagcag actgagccca
    42181 attcggtctc gattctctgt tcagcaagcc tcggcagcgt gtagtttgac ttgctgtttg
    42241 aaaagccaaa acccgctatg gtacagtcct gctctttcaa ctgcctcgca gattgcctct
    42301 ctggtcccat cgtctagctt ggcctaggac ggagccctct caaggctcag acacgggttc
    42361 aaatcccgtt gggaccacca acctttcccc tgatcttcca atgagttaac gaccttcttc
    42421 tgatcagcca tatccgtggc cagccctcca taggacaacc tataggacaa attcgagctt
    42481 attgggccga tcttatacgt attttcaagg agtgtcacag cctccctcag tttgagattc
    42541 cagccctgcc cgccgtgcga atattgactg gtcatgaccg ccttggtgct gtgccctaag
    42601 agggccgaac gtacttctag gctcacccca agattctgaa gcctggtcgt gaaggtgtgg
    42661 cggaggtcgt ggaagtgcag gtcctgcacc ttggctgccc tacaggtccg gtgccactga
    42721 tggccgaacg catcggcgga ccaatttgcg aaaagaggtc cttcaatcgc tgccacctca
    42781 gggcgtagcg cacgatgggc cacagagttc aacggtatct ccctcggcgt gcccttcagg
    42841 cgcgtcctgg aaggcggcag gatgagccac cacccatcat cccgctggcg gatccaggat
    42901 cggtcgattt cgaggatctt ggcctctctg aggcctgtat ggagggccac ctgggcaata
    42961 cgccagaact cgcccggatc atagacacgc cctagaatct tgtacggggt tcgtttggcc
    43021 gcttcggcct ggatcgccga cagttcctca ttcgtggcta cccgttcccg gcgggccacg
    43081 tcgggaagtt gcacacgctt gagacggttt cgatccagct tttcaaagtc gacggcaagg
    43141 ttcaagatgc gcatgagcac gccccattcc ttctgaatgg tccctggcgc tgctttcgcg
    43201 tcgagcctgg cggcgatgta ctgctgacca tcctctgggg tcagtgaatc cagcaaccgg
    43261 tctccgaaac ggggaaggag atgcgtctcg aggatgatct ccggccggcg aagatctaca
    43321 cggtttttca ctcgtagcgc ctgccaatag gttggaagaa atgccctgag tgtagaggcg
    43381 ggagtcctcg acggaggagc ctgttctcca cggtggatgg tgctgatcat ggcgatcgcc
    43441 agtcgctcag cctcatccgg tgtgagatcg tatcccaaga gcggccgata ccgctgccct
    43501 ttccagcggt agtagaggtc aaaggcaagg cccttctggg tccgtcgttt ggtgatggtg
    43561 taggacatgc gtcttatcga tagcgtgggt tccgatggta aacagtgccg gcgtttctgt
    43621 ccagggggaa cgtcagcact tcaggtcctc ttgaagcagc gaccacatca ccgcttttca
    43681 gccaatccaa cagctcatct ctcaggaatc gcacctcgcg tgatccggga atccgatgcc
    43741 ggggaatatc tttccgttgg tacagcgtgg atttcgacaa ccgaaggtat gcggccgcct
    43801 cggcgagcgt catgatttct gggacgctgt gagtatgtgc ggcctcgggt gtcatcgtgc
    43861 tccgtccagc ctcaaaaaaa tcgccctggc ctcggtttgc aattccagca gggtgtcgta
    43921 ggcaaaccca tcccggaggc gccgccccaa ccgattgacg ttcgaggcaa tcgcatccag
    43981 cagttcatcc agtttcttga gcagttcgcc atgtgcggta gtgccggcga gctcgatcag
    44041 aatggcgtct ctgatttggc ggtcgcgatg agccaggccg gttgtaatca ggcccagtgc
    44101 cgtttcgatt cgacgcacag ccaggcgacg cgcttgggct ctcccgaggt cttcggccag
    44161 ttgcatctca ggtgtacccc agcttggtgt ggatcacgtc gggcatgtgc ttcacgacct
    44221 gcttggtcat cagccgccca tcgacgtaga ggttgatctg ctgcgtgccg gtgccgccga
    44281 agcccatcgc ggattgcatg aacttggcgc cgcgatcgtt cagcggaatg gcggcttctg
    44341 cggaaccggc ctcggcaaat ttgccgatgg tcgggccggt catgatgccg ccgtcggcga
    44401 atggtgtagc gagggcaccg gttgaggcca cgatggcgcc cccaagcgcg gtattgagcg
    44461 cggccgtcgc ggccgtgagt gccgcggcac ctgtgaactg tgcgatgaat gccgctgaca
    44521 agatgctgcc ggcgagggct tgtcccacga cgggcgctgc ggcgacagcc gcggcgacgg
    44581 cggccatgaa ccctacgact gcttccagga caatcccaag cgttgctaat gccgcatttc
    44641 ccgtggcccc gatcgccacg atgctcgctg tcgaggccgc tagcatggcc ttattggtga
    44701 gcactgtgat cgcgagccgt gaggtctctg cggtagcggt ggccgccgtt ttggccgctt
    44761 ccagggccgt gtgttgagtg agctgggcac tggccgctgt ttggccaaag atgagttgtt
    44821 tgccttgttc gaggataaag gcgagcgtgg cttgcacggc catgttcaca aacgatgtca
    44881 gaatggtcgt ccacactgat tggaggaagt ccgcgaaggt tttggtaccc tgcagcatcc
    44941 ccacaatcgc ctgcgagaag ctctggacga ttgacgacca agtgaaggcc gcactctgtc
    45001 gaatggcgtc catttgctca gtgaaaaagc ttttatgttg gacacccagc gcgttgagct
    45061 tgttcgtctt ggcgatctcg atattttcaa tcgccatcgc ctgcagttga gcatccttga
    45121 tggtctcaac gacctgccgg cggcgggcct cggccgtccg ctcgatctcg tagcgttgcg
    45181 cttcttcacc cgtcatggcg gcttgccgta actcttcgaa cagatcgagc tctcgctttt
    45241 ggtcctcgat cttgagcgca ctgagcctca tcgacttggt gcgttcgatg ccctccaaga
    45301 gggctgcccg ttcagtctcg tcctggatgt tctccttcac aaacctggct ctggtcgcgg
    45361 cctcttcttc gatgaccgtg atctgcttcg cccgatcgtt cagatgttca agcatgaggc
    45421 tgtgctggag ggctgccgtt tgcttcgcca tctcctgctc atccttatat cgagcgttaa
    45481 tgaattggcc gagctgctct tcggctcgac cgctggccaa ttcctcacgc gcagccgtga
    45541 gatcttggat cagctgcaaa ttccccaacg cttgctcacg ctcggccgca taaaaatcga
    45601 gcgccgcggc gtcctgattc gcgccctgga ctgttggaaa cgccttcatg gcgtggctgg
    45661 ccttcttgac ggcctggacc gcctgctcaa cctttttggt caccacttcg atcgggccga
    45721 tcgaattctt cgacatcagc ccgaaatcca tcgcaatctt ttcggtgtag agattcagct
    45781 gatgacgatt tttggccagc atcgcctcca gcgggttggc gtgttcctgg aagatctttc
    45841 cccccaagat ctcaggcagc agagccccca gcttggtgat gttcagggcg aactgctcca
    45901 acatcccgat catcatggta aaggtcgata cgaccacgta acccagtgcc ttcatgccga
    45961 gggtgaggtg atcggtaaag gcactgttct tgatctcaat cgacgttttt ccgatctggc
    46021 ccatgactga aacgagatca gtcgcggcgc ttacgagtgc gcgcacatct ttctggatct
    46081 cgtctttgat ccactctcga ttcgccttga cccaattcag gaccccgtca gtgacatcgg
    46141 tgagaattgg gatgagcact tgtgcgacat cattcataaa gccttctgcg acatacccca
    46201 ggcgggtgag gctgtcgtta aacgcttcgg cgccctgcgc ggcgtcggtc gtccaggcca
    46261 ccccaagcgc tttcgcttct tccatctgcg cgcggatggc cgcagagccc tggttgagca
    46321 gcggcagcat ctgcatgccg cttttgccga acatctcctg ggccatggcc gcgcgtttgg
    46381 tttcatcttc catgcgggcc agctggtcag ccgcatcgag catcacgtcg tttaatggcc
    46441 gcagcgtgcc gtcggcctcc ttgtacgtga cgccgagcgc ctgaaaggtc gttctggcct
    46501 gcgtggaccc cgtcgcagcg tcttgttgtg ctctggcgag cttgcccatg ctcatgctga
    46561 gctgctccat cgagacgtcg gacaggtccg ccgcaaaccg gagcccggag agcgtttcta
    46621 ccgccacgcc cgtcttttgg gaggtcttat tgagctcgtc tcccacgacg gcggaagact
    46681 tggtcatcga aatcaatgcg gctccggtcg tcgccgcgac cgcagtcaat gctccgagcg
    46741 cgagcgtgcc aatcttggcc gcgctggaaa cgagctttcc gaaatcgtcg gtgttgcggg
    46801 cagcgcggtt cagttccttc tccatgttgg aggcatccat ttttagcctc ataatgaggc
    46861 tgcctgtgtc acccattctt cacctcctca aaaggcacat cttcataggg gtacccaaac
    46921 ccggcgcaga tggccttgat gactaaaaat ccaaatcgtt cgtagttgtc ttgtgtgatc
    46981 aatcgtcgca cgagcttgag ccggagagct gggcctcctc gttgtaatcc tgcccagatc
    47041 atgtgctgag tgaaggggag atcaaccggg tattccagtg ggcgtgaatt tttaaacagt
    47101 ccaaattgat ccgtcgctaa gcgaatggct cccgcgtcga tcaggagttg acgtgggcca
    47161 ctgagctgga tcggtagact tcgcggagtg gttcggcctg gtaaatgttc cgtcatgcgg
    47221 acagctcctt cttccgacgc cgacttgtct gatctgtgct cataggcctg tcagttcggt
    47281 catcgaccgc tgcaaatatt cccggatgaa ctggagcagc tcggcgcgta agacgtaggc
    47341 ctcgccgatg ttgtctcggt agcactcgag ctttccggca gtcatccaat actgaacagt
    47401 cgatcgatac actccgagca gctgagctat ggacgccggg ctgaagcagg gaaaagtcgg
    47461 tatcacgatg gggttgggtt gaacaggaat cgcagtcacg ggcgccggcg gacctgtcga
    47521 acgctgtttt ccggacttcc gtggaaccca tccctctcga cggcctcctg tccatgtcat
    47581 cgatcacgtt tcacccagct cgttcgtggt cgcgcttccg ggttccgatg gacccagctg
    47641 gatcgatccg gatcgaccgg tgcaggcgcc ggcggtggct catgtcgcat caccgttaag
    47701 gtgtcgaggt tcggattgag gatggcgagc gccgtcatgg cataaatccg cagatcgagt
    47761 gcttcatttc ggcttcgctt cttccggtaa tacgatccca tcaatacgcc gcgttcccac
    47821 ttgttgacgc gttcttctgc tgtgagttgc ttgaagtatt cgaattcgtc ttcggtgtag
    47881 gccgtgaggt ttgggaaatg gcagtaccct gggccgaagt cttcgagctt caagcggtcg
    47941 aacagcgtgt ctttggaggt atccgttccg gccaaaaaca ggcgtattcc gttgacggtg
    48001 ctgcccatct tgtagacagg tgcaccgggc tgactggagc ccttcacggc ccaaatgcga
    48061 cgattctggc ggggcaacac gaactcatag acttctttcg cgtggtgtcc ggtgtcgata
    48121 caggcggttg cgacggtgag aaaccctccg gtctcatgat cccaggtgcg cttcaaccat
    48181 tcgtccacct gatcccagag ggtcttgtcc gttggcgatc cgaaccattt ccgatagtcg
    48241 atcgaccagc tttcttgtcc tttgccccaa ccgacgagct ccgcctcaat gcggtccttc
    48301 tgcacgtcga taccggctgt gagcaccacg accccgtttg gtactggcgc cggataaatt
    48361 tctactcggt tgtgtaattc ggactcctca atccgttcgc cggcttcctc ccacgtctcc
    48421 gccaggttcg tgttggtaaa ggtctgctgg gcacggaaat catggttgtg aatgatctcc
    48481 gtccactcag aggctaactt agcccagctg ttgacccagc cataaggcgc atagagcgca
    48541 ttgatccaga agccggcggc cttcagcatt ccgccgttgc cagcggcctc agctagccat
    48601 ttaccatctg cgagcatccg tggcttatga gcttcgccga tgtattggtg acagtgctcg
    48661 cagagaaact tggcttcctc tggctttcca tcaggccagg caagattttt ccaccagagc
    48721 acttggccat ggtcacaatg gggacagggc acaaagtatt tccgctgatc actttcgagg
    48781 taagcctttt caattacact gcttcgcttc aggagaggcg tggagcagcg atatattttg
    48841 tgtcgcgcat aggtcaaggt ccgcttttcc gcaagctcgc aagggctgcc ttcgccgttc
    48901 acgtcgggca cgtaaccgtc aacctcgtct aagaataaat ttttggcact catgaacctg
    48961 aggcctggtc cggagttggc tccggtcaga catagaatgc cgccggtgaa ttccttgctc
    49021 atgatcgtgt tgcctgaatc tcgcgagcgc gactcgcgaa cgagtccgtc gaggcacgta
    49081 accgtctcga tcattggttg aatacgctgt ttggaaagct tcgctgccat atccaaggta
    49141 ggttcgacca ggagcattgg gcatggggct cgatggatcg tgtatccgat ccaatttgtt
    49201 gcggccgccg ttccaccgac ttgcgcgggt ttcatgaacg caatttcacg gactggcgaa
    49261 ttgggagaaa gcgcatccat gatttctcgt aagtaaggca cccggctggt tcgccagggg
    49321 cccggttctg agcaatatga tgaagatagg accatgtgac ggtccgccca ttctgaaacc
    49381 gtctcgatcg gatctggtgt caggcccttt aggaaatgtt cgacggcttg ggatgggtca
    49441 gcgagttcaa tcatgtggtc tatgccggtc ctttcgtgcg gtgtggttgc cgaaaacctt
    49501 tcttcaacac atcccgcaaa agctttctca cgacttcaat ctcagaccgc cgccaggatt
    49561 ttcttagctt taacatccgg taatctgctc agcatttcga gcacctgatg aaattcactc
    49621 ttgagcaaag aaaagacggc cgcctggttt gtctcggcag cgagcggccc agccaaccgg
    49681 tcgggcaggt tctctagggt gtcacgaacc tgccgacctg ccgcgaacca ggcttggttg
    49741 accgcatctc tcgacatgag ttcgccggag gctctgtcca atttcaaccg cgtcatggcg
    49801 ttgccgagcc gcagcttttc cgtttcttcc ttctctcgct ctccgctggt cttattctgc
    49861 tggacgtatt tgaggtaggc ggtgacagct gctttaggct cgtgctgatg tgcgcgaacc
    49921 ggcttaggta gaaccccttc ctgaaccagt tgggtgatac gcatcggcgt gaggccaaga
    49981 actcccgcga gttcctgtct cgtccatgtc accgtcccga agagatccac cgcttacctc
    50041 aagaaagaaa gtgccgattt atttccctgt gcctgcccat gtatcgcgcc gcaggccacc
    50101 cgcatggcgc atttgccggg aaggacccgc gccggggggt ggttggtaat caatcaacgc
    50161 agaggcccgc cttcgttgcg agcattgagg aacggctcct catcattgct ggccttgaag
    50221 cagatgatcc cgatgccgcg ctccaagctt tccacggcga agagcgcacc aatgagcacg
    50281 cctttgccga gcacgttcag cggctgtctg cgttcacccg gctcgccata gaccagcgtg
    50341 ccgcgtcgag actcggggcc gagcccttct actcgaccat ggaagcagcc tcgtgtgaac
    50401 acccccacga tcactcgact cttgttgtcc tgtcccacgt tctctgcgat gccggcgaat
    50461 ggcagggttt catcgaaaga gcagcatttc ccatctagcg cagagaccag gtcccctcgc
    50521 acgatgccat ccaagggctc ttcggggtgc agatgtagct cagctcgttt caatggctca
    50581 acagtgttga agtgcttcgc gttcagtcga agcaccttcc gggcttcatc gagttttgcg
    50641 ctaaccccac ccatgatgat ttgtgtcggt gccatctgct gattctcctt gttggcgaaa
    50701 ccgtcgccag cggggccgta gccgtctaag ctgtgcaggg ttgacgggat gccagcgctc
    50761 accgttttcc ttgctcatcg cgtgcgacgc gtcgagtctt ctgtcggcag aggtttctcc
    50821 agtaggcttc gaagcgttcg atcgtcccga actcttgccg taacgtgacg ctgtgctcat
    50881 aggcaaaatg gagcagatca agagtcggta tcttcgcgag ggctgcatca gaccacgcag
    50941 tctcgttcat agctcgcagg gtctcttgtt gatcgcgcgc catggtcgct cggagtgtct
    51001 gaagccgctg cgcgtcctga ctggtggtcg tcggttggac atgcttgcgc cgctgagaca
    51061 gaatctgcgc aattgaagag gcgagagagt tcgagaaatc gttcagaatg ggctccagca
    51121 gttgcgtcgc cttcgtaaac acttggtcgt tgatcatgcg tgatgcctcc taaaatccat
    51181 cgtggttggc acgggtcgtc ctgttttcca gtgaggaccg gccgacaagg agatcgaccg
    51241 gccctcgccg tgccggcttc gtgagtccgc tcaaccacgg tgcacggagc cggatgtcaa
    51301 agtctgagag cagcacgtca ctctcggtga gatcgaccgc ttcgaactgc agagcgttcg
    51361 agaaatgggc cgagagttgt tgacggaact gagcgatggc ccgctctgaa gagttattct
    51421 gccgttcgct caatgaagcc tccgacagat ttctttaagc gttgatccca gcggggagaa
    51481 ctcttccacc tcgcgcagat gtggaggtaa atcttcttct cgcatcgccc taccaaccgc
    51541 atttcttccc cggcctcttt cttgctctga agatccggat cttaagattt ctttttcttc
    51601 ttttcggttc ggatggttcg gaaagggaag gttaggatag gagggggcca tgattgttcc
    51661 tttgtcggct ccttccatct cggcttttgc gtcccgccgt ttgtgatatt tccagtagtt
    51721 aggtgattca agaaccaacg gagatccatc cgagtcttgt tctttaactg ataaccaacc
    51781 tttcttggtc atccaaagaa ccgacgcaga tactttcttc gggtaaagtc ggacctttct
    51841 agaaatggtt ccgagccaat ctccgttgag tctccagtgg ttctccgttc gatctaggat
    51901 ggcaagcacc tccagccaaa tccgaatggc gcgatcacca aactcctcgg tgaacgccca
    51961 cagctccgcg tcgtcgttta gatctcttga gaccggatgc caacgtttcg gagagggcat
    52021 tatttcctgc ctgcctgtaa acgcattcgg tcctctgcga cggtttctcg ttctacgcgc
    52081 agccgttcca cgtcgcagcg cttaaatagc cgcaagcggc cacagcgaat ggcaggcaac
    52141 acgcctttct tctcaagtgt tctgatgtgt tccgcgctta tgcagagaag gcgagataca
    52201 ggcccgattg tgaggaaatc atcttgttcc atgcttcaga agcatagggc aagattgtgt
    52261 gataaaaacc gtcctatttc agtcggtgga ctggtcggtt ttcatgtggc gatggtgtta
    52321 gtcaaaccaa ggccagaatg gagggctgga atcagttaag acggcacgaa ccactccttc
    52381 attatcgacg atttttttat acctaggcgc agagtcgttc ggcttgatgg aggatggcct
    52441 gtagtgcttc ttaatcacca cggcacttgc agcgttatgg atcattgaga gagtctcagc
    52501 gatgaaatcc gctcgctgga tcgtttgttc attattcttt ttaaattctt tcagacttgg
    52561 gattttcagc tcgtcgctat aagggcaata gcatggaaac aggcgaaggc cctcgaaaat
    52621 atggccacga tttttgcgca gccaatgcca tcgatcctgt gcagcatttt tgcagttgtc
    52681 atatccattt gtgtggagga accagtcaag ccagttacgt tgcagtacca cgctctctcg
    52741 aagatgatta cccccatcct tgtgtcctgt agagatcttg cgcaggaggg acccaaacaa
    52801 ctcagcgtgt cgggattcaa tatctttctc agtcatttga aaatatgagg gacggctatc
    52861 ccagtagagt tccctgacag caattaaata aacccacacg gctaattgcc tggtgtcagt
    52921 cttgcccttg gttttatcgt atttactcaa gcgatgcagg agggcctcga taacctcctg
    52981 tgctaaatga agccttcgtg gaacgtatct cccactcttg ctcattcgat tgaaagctcg
    53041 atagatccca gacggaacgc ctaaagctag gacgcacgcg ggagcgctat aattcctgat
    53101 ccactcctct gacaaagtgt ctcttttctt gggtctagcc attgtctctc ctccacttca
    53161 gtggatcact acgcacgcca catctgcacc cagacttacg gtacgagact taatactggg
    53221 acggctgcgg aaactttctt ctggctaacc ccgagattta acaggccttc ctctgcgtgg
    53281 tatttagcaa tcgcccagat cagctgccgt tctaaattgc tggccgcgtt agcccgtcga
    53341 gaaattggag tctggcgctg aagctcttcg tatatttcga ccacatcttc tggcaattga
    53401 aaggacacgg cgcggtattt cttctcactc atttggatgt ctcctttgtg gtctcctgca
    53461 gatgaggaga ggtgatttgc tgaagctaat tttgttgaag gcatggactc aggtacggtc
    53521 actaagcaag acgaaacgtt atcttccagc cgggaaaaga tcaacactcg ccaggggagt
    53581 tgtgctgcag tggagatact taaactcacg aaagattttc cgtatctccg gttttcaaga
    53641 ccggcacgtt tggggaattt ctcatgctcc aaggggcttc gatatgggag ggagaggcca
    53701 cggttcaagg aagccgcgtc acgctggtga tcagccggcg cctagccctt ccgattcgcg
    53761 ttgaacattc cgctcagcta cttggggagc gaagaaacct cccggtaagc cttcttgatc
    53821 agttcatcgg tgtagctcac ataaagtccc agaaatattg aggcatcctc cacgaggccc
    53881 tgttcgccgc tgtcgaccag tcgatcacga aggtttctta gtccaatttg cgcagattcg
    53941 aagtttccca gaatgccctg caatccttca acgggggtat cgcatgagag gctcatcagg
    54001 tcgctcattg tcgggctcct ttcagttgta gtggagggga cagtgatgtg aaggctgctc
    54061 gattctgctg ctcggcactc tcaatccacc acggcggccg ttgattgctc accgtgcagc
    54121 ctgcgggcat cgggcaaggt ttgaactcgc gcactcgctc gtcttgcgct gcctctctga
    54181 atcgctccat gaccgtctgt tccagcgccg tcacggccgc gcggagcttg gttttccaat
    54241 cgtgaatgta ctgctcgccc atgccgggca ggcgatgccc gaggagcttt tcgatcgtcg
    54301 catactcgac tctcgcctcg ccgagccagg tggccgccgt atggcggaga tcatgaaaat
    54361 gcagatcatg cacgccggcg cgttccgtcg cctcgatcca gcgatgcttg aagctccccg
    54421 cgtctttcca ttgggagaaa taccggccac cgatgcgtgg ttgtgttcca cgtagaaaaa
    54481 agccggccag ggaggagaga ggtggctccg gttggttgga cagcctaggc cattccttaa
    54541 gtggcgcctg
//
LOCUS       DAORRQ010000023        54339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_33_length_54339_cov_50.748489, whole genome shotgun sequence.
ACCESSION   DAORRQ010000023 DAORRQ010000000
VERSION     DAORRQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 54339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54339
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33_length_54339_cov_50.748489"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PK224_09315"
     CDS             <1..1883
                     /locus_tag="PK224_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRB15975.1"
                     /translation="LRRLTQPFLLSFRSGLHVGCVLTGLLLFVLLCVDMGYYGFNRQH
                     MDFVFLEYVGDLFAPAATSDSTNVQAMQQTSAELGAAGKWAGRLALFLGIQAVAMGLW
                     WWSFSCAIVPALARWRPGSGFQANTFLLVALVAGGAGFHHSGPYGIRIAPIGSMVYYT
                     LAQNPVLFAAEALRIAVVSRGSMEQRASAEAMPYEVAVQTVQRLLGPGEAFVDQRYPL
                     VRTMAASSDGVRLPGQANILLVFLEGLDRRYLEQKYGEIQGTPFLDRLKQESVYFENF
                     FSNGVQTSRGLFATLCSAFPRQGAAAMKTRYAHDYLCLPSLLQRQGYRTEMVIGQHRD
                     LNRLQSFVARNGLQQLIDEGDFPKDAERMGLGIVDGALFDLCYERIKQRQAEDKPFFL
                     TTLTLSTHHPFAAPQNHPDVRLLQEQVQDKYVGALRYTDLEFERVFTRLVREGLLRNT
                     VVVVLGDHGRHEPVGSTDMERKAGHFASPLFIWMDESLRTPATYRPRTVSAIASQVDV
                     APTLLALNGILPAVGAFAGHDLTCLLVRDCLPNQVAYLTSVYDNLVGLASPDGLLLYS
                     FSTESFQEATLNFDAVSDSESGRRRRFATHDLELMALYEVANVALDSNRLWSWREFGT
                     RL"
     gene            1937..2734
                     /locus_tag="PK224_09320"
     CDS             1937..2734
                     /locus_tag="PK224_09320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB15976.1"
                     /translation="MNHIPVSAVVIAYNDEPNMRACLESITWADEIIVVDSHSTDATE
                     RISREFTDQVYQHDFHGFGRLRNEALTHATHDWVFSLDTDERATPELRDEIRRVLAAG
                     PQADAYFVPRKNYFLGRWIKHCGWFPDYRQPQLFRKSRLRYREELVHESFDLDGKIGY
                     LTAAALQYPFRDIDHYLAKQERYSDLMARRMVEQGRAFSPHQLISHPCFTFLKMYVGR
                     KGCLDGVPGLILSGLYAYYTFIKYARFWELRSRVLTEGSQTAPVKQG"
     gene            2737..3534
                     /locus_tag="PK224_09325"
     CDS             2737..3534
                     /locus_tag="PK224_09325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB15977.1"
                     /translation="MTVTDGGAERRTMTIGAVVITKDEEKNIADCLESLRWADELIVV
                     DAESRDRTVELAKQYTPNVFVRPWPGYGPQKNFGIDQAAAEWILVVDADERVTGGLRR
                     EIEALLATAPAADIGGYEIPRRNFFYGKWIQGGGLYPDYQLRLFRNTAGRYDDVKLHE
                     NFTLSGRRERLQEPFDHYSMPTVNHHIRKMMRYTTLGADEKLKRVTHISGWVIATHHL
                     GTILKTLFSRGGYRDGVHGLVVAMFAGLHTFVKYAKAWERVNVRRDA"
     gene            3584..4687
                     /locus_tag="PK224_09330"
     CDS             3584..4687
                     /locus_tag="PK224_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRB15978.1"
                     /translation="MRIGIDAASIVGDKGGVGWHTYHLLRSLLALDEQVEYVGYLRPG
                     SLQAGRLEGWPVHDRLRWVETPRWLMPCRGAWDRLDLYHGPNFKMHTTGRSGGIVTIH
                     DLWLARHPEYSRKLLGQAGSSRRAIATANRARRVVTVSEFSAREIEALYGIPRERVVV
                     IHNGVTEEFSPVQDEQGMAVLRARWAIPSAGFILFVGGADPRKNHRVFLQAVAQSRSQ
                     LGGRAILLVGDAEHPQGSYLATARSLGLEQDVRCTGRLNREDLCRLYSYAEVFVFPSL
                     YEGFGMPVLEAMACGAPTITSSTSSLPEVAGDAALLVNPEDAEALGAAMVKVLSEQAL
                     RQQLRDRGFERARLFTWQRAALRTSALYRELCA"
     gene            4832..5950
                     /gene="rfaQ"
                     /locus_tag="PK224_09335"
     CDS             4832..5950
                     /gene="rfaQ"
                     /locus_tag="PK224_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HRB15979.1"
                     /translation="MRFSVVLNSKGVGIVLQRAAKSDGVMDLQNILIIKLRHIGDVLL
                     STPVLRGLRTAFPRARLTMLVNRGTEGVLANNPDVNEVLCLEKGAWDAQLKFVQMLRR
                     RGFDGVVDLTDGDRSAVISLATGAPVRIGFNAEHRWRGLLYSTVAKPRPTDQHRVDYD
                     LCALRALGLDTKPGTPALYASPADEQAVEAWLREAGLLSAQSSQLLILLQPGARYSLK
                     VWPHERFAQLADRLADRFACRILLGGDQREREVAEQVARKTRCAPIVVAGKFSLLQFA
                     ALVKRCALFVGNDGGAMHIAATMGTPVVAIFGPTYPQRWGPRGGPAQVIYKGLDCRAC
                     YHPTCLRGDDSCMQQIAVDEVFTAASRMLERTPARTET"
     gene            5947..6846
                     /locus_tag="PK224_09340"
     CDS             5947..6846
                     /locus_tag="PK224_09340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB15980.1"
                     /translation="MSDELVSVVIPVFNGAPFVAKAVASVRAQGHGAVEILVVDDGST
                     DGTQEVLNRLEQSDGIRWFQRSHGGPARSRNYGIAAAQGQFIALLDCDDIWLPGKLAA
                     QLAIMRARPDVGLVHTDFEVRFEDGTLEERVSARSSREPMVQAFAGGHVALPSTLLIR
                     KSVLDQIGSLDPELYGSEDSDLTIRLFRVTRFECIDEVLVRKLQRGHGYRDMAFDEQT
                     HRERVLASRDRFLMRLESFAPLTAVQRAALDREWANYFLLKGAAAERAGRRSDARRHY
                     WEAICKAPARVRCYTRWLRTLKP"
     gene            6914..7930
                     /locus_tag="PK224_09345"
     CDS             6914..7930
                     /locus_tag="PK224_09345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB15981.1"
                     /translation="METISCDVCGEASNTALFCQYDLTHHVTDDLFTVVRCQGCRLLF
                     LNPRPTREEIGGYYPSTYYPEAAPRQAGDLRRTAKRWSGKIRRWIAQDFYGYPAPESA
                     RRWQWARRLLLWPEFLWRRWRGRGLLPWVGQGRVLDVGCGSGGNLAVLQEQGWNVSGV
                     DASAVAVAQAQARFGERVRQGDLASMAYPERTFDTVLFSHVLEHLHDPLSLLKEVWRI
                     LDWEGRVVILCPNAGSLEARMFGRWWFPWELPRHLFHYERATLTRLLEAAGFQIESVS
                     TGLGSLYFMASLDRLCEERFKRAVPWRRLIEKVLIRPFCFCVGHLGHGTELKVYARKG
                     RSAG"
     gene            complement(7927..8802)
                     /locus_tag="PK224_09350"
     CDS             complement(7927..8802)
                     /locus_tag="PK224_09350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRB15982.1"
                     /translation="MAQISVVIPAYNGTSRYLKEAIDSVRAQTLAAGEIIVVDDASTD
                     GTGALVQAIPQVRYVRHPQNSGQAAARNTGARLAQTPYLAFLDQDDLWEPTFLEATLG
                     ILESAPQATLVHCDGYQVNERNEILEYDAAMKQQRSITQLLRGGHDAATSGSLFRKTC
                     FDAVGGYDAALSIWEDIDLIIRLYGPGRFIHLPKPLYRHRLYGHNASRNIPSLRALDG
                     RRRFLDKHAASCRPGTPEGDALSHDWAIYYADLGKHHLRANTPTAARQAFQSALRHDP
                     YNVKTWSRLVRSYFV"
     gene            9056..10135
                     /locus_tag="PK224_09355"
     CDS             9056..10135
                     /locus_tag="PK224_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRB15983.1"
                     /translation="MKIVIAAWHLKNLNVGIGRYARELIEALGRVDQTNHYEILIPHA
                     EHPFTARPNMRYRVIRFPLFRRRFWEQVAPLLLGPYDVLHFPYDSCVAWKRGKFVATI
                     HDVKPLLFPVLRARTNLNSRIEQWLIGDRWKKIDHVITISENSRRDLLAHVPLRPEQV
                     TVTSLGLDTERFRPAEHRGEGKPYVFCVAGADPTKNVGVLIDAFAKLPETLRSRFDLL
                     LAGDVCKRPDIRAAVEHHGLGAHTKLVGMVSDRELVAYYQQATVFVFPSLYEGFGLPV
                     LEAMGCGCPVICSNASSLPEVAGDAAVLFDPRDGGRLALELSRVLASPALRDELRARG
                     VARARAFSWDRTAIETVAVYRRLAG"
     gene            10205..11461
                     /gene="lhgO"
                     /locus_tag="PK224_09360"
     CDS             10205..11461
                     /gene="lhgO"
                     /locus_tag="PK224_09360"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HRB15984.1"
                     /translation="MLSWVSDRFLPDVEGMHDMAEETCDVLIAGAGVIGVAIARELRR
                     RYGVKVVVLEKESTAGRHASGRNSGVLHAGVYYKAGTLKARLCVEGNRRLRAYCRQQA
                     IPLNEHGKVIVARTEAELPALRELYARSQANGVRASLLDQQTLKEVEPCAKTVQQALY
                     VHDTAVVNPRLVMAAMVADAEREGVEFRFGCAWQGRDGDGVARTDRGRVTYGHFVNCG
                     GLFADRIAHAHEVGLHFKILPFRGQFYQLRPGSAMQVRGNIYPVPDLRNPFLGVHFTR
                     RPEGDVTIGPSALPLLGREQYRGLTGATLGGTVTMLSYLMRLFGRNRDHFRSIAWTEM
                     IKLTRTGFFREAEDLGRGFEKADLLPGQAPGIRAQLVDTRTAELVSDFVIEPGARSTH
                     VLNAVSPAFTSSLPFAEHVVDTMRLA"
     gene            11760..12494
                     /locus_tag="PK224_09365"
     CDS             11760..12494
                     /locus_tag="PK224_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB15985.1"
                     /translation="MKGVVLAGGLGSRLLPLTKVTNKHLLPVYDKPMIYYPIRTLVNA
                     GITEVMLVTGGNSAGDFLKLLGNGRDFGLKHLSYTYQQGEGGIADALRLAEHFADEGP
                     ICVVLGDNLIQGNIAKAAEAFRVQKRGAKILLKEVKDPQRFGVPHLEGDRVLSIEEKP
                     AHPRSSYAVTGIYFYDARVFEITRTLKPSGRGELEITDVNNAYIASGELTWNVLDGWW
                     TDAGTIESLLAANQLVAQTGANRMEF"
     gene            12495..13523
                     /gene="rfbB"
                     /locus_tag="PK224_09370"
     CDS             12495..13523
                     /gene="rfbB"
                     /locus_tag="PK224_09370"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRB15986.1"
                     /translation="MRILVTGGAGFIGSHLVRGLIQSGHHSVVNLDALKYSGNLENLA
                     DLAGHPQYTFVHADIGDRQAVHAALQTHRIEGIINCAAETHVDRSILDPGAFARTDVV
                     GTGILLEEARQAGVQRFLQVSTDEVYGSVEQGSSTEGDRLEPRSPYSASKAGGDLLVL
                     SYWTTYRFPVVVTRGSNTYGPNQYPEKFIPLFATNAIDGEPLPLYGDGRQCRDWLSVY
                     DHAAGIQHVFEQGEPGTVYNVGGGNERENITVAEQIVATLGKSRSLIRFVQDRPGHDR
                     RYSIDCRRLRALGWSPQVPFEEGLKQTVAWYRTHESWWRTIKSGEFKDYYQQQYAKRL
                     QQGTACGL"
     gene            13511..14362
                     /gene="rfbD"
                     /locus_tag="PK224_09375"
     CDS             13511..14362
                     /gene="rfbD"
                     /locus_tag="PK224_09375"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRB15987.1"
                     /translation="MRIVITGAQGQLGTDLRQVLQGHQLTLLDLPTFDLTHQDCGRVI
                     VEAVPEVVIHAGAHTDVDGAERHPALALAVNAEGTERVARAAALVGARLIYISTDYVF
                     DGRGTRPYVETDPTNPVSAYGESKRAGEQRALACCENTLVVRTAWLYGLHGRNFVKTM
                     LQLASERPCLKVVADQRGCPTFSGDLAGMIGKLLAHPAQGVLHVTNAGNCTWHEFATE
                     IVRLSGRPVPVEAITTADMPRPAKRPAYSVLSADRLHHLGFSMPSWQDGLQRFMKALP
                     AVSSAVS"
     gene            14893..16230
                     /locus_tag="PK224_09380"
     CDS             14893..16230
                     /locus_tag="PK224_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB15988.1"
                     /translation="MTSPPADSALRSVHTDARSVSGHALFWAFQGYVMAAVACSSFFP
                     MLFRYQEHAVIVLVVLSLGMCAIERVSPWIKNPLDLPLWLFMIWVLCTVPFATDSAYS
                     FAEWKKFVAQAGVFYWSLLVLDRCRRENLPQQILWALALTGAALAVYSLVDFVDRGGT
                     WRDRYIRANAFGSDYNWLSTYMVMTIPVVGSLIVLGRLVWFRAAQALALALMGGAQLF
                     SYTRGGWLGHAAQGIAFAAMVGGRRLALWVLGLFAMAGAGLLVASQAGFQRDTVAAST
                     VDTRLTVWAIGLRQVATHPVVGIGYGNNSFVKKFPEYSSEAQAQVSEGTRIIPAMHNT
                     FLMVALGSGLPALLSFVWIFVALLRRLIPIPWRAAWNDRWSVLAAGIGLAVIGFAVRN
                     LFDYMFMGSLAHIFWLLAAVGITVTGSAWRGGGSGRQPGSPQDEGTSQQQPSA"
     gene            16588..17595
                     /gene="gmd"
                     /locus_tag="PK224_09385"
     CDS             16588..17595
                     /gene="gmd"
                     /locus_tag="PK224_09385"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRB15989.1"
                     /translation="MNALITGITGQDGSYLAEFLLAKGYDVYGIIRRSSSFNTARIDG
                     IYQDPHTSGAKLHLVYGDLNDASSLNKILRTVQPDEIYNLGAQSHVRVSFDIPEYTAE
                     ITGLGTVRLLEAIRESGLRPKFYQASSSEMYGKVQEVPQRETTPFYPRSPYGAAKVYA
                     HWITVNYREAYGLFACNGILFNHESPRRGETFVTRKITKAAARIKLGLQQELFLGNLD
                     AKRDWGYAGDYVEAMWLMLQQDEPDDYVVATGETHTVREFLDVAFGQLGLDWQRYVKI
                     DPRYYRPTEVDLLIGDPAKAQQKLGWKPTVDFHRLAIMMVEADVEAERLKLQGTASKG
                     A"
     gene            17596..18546
                     /locus_tag="PK224_09390"
     CDS             17596..18546
                     /locus_tag="PK224_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRB15990.1"
                     /translation="MIDTHARIYVAGHRGMVGSAIVRALEARGCDNLLFRTSKELDLR
                     DNRRVAEFFAETKPDYVYLAAAKVGGILANSTFPAEFIYDNLAIQTNVIHHAYVHGVK
                     KLLLLGSSCIYPRDSPQPMKEEYLLTGPLEPTNEWYAVAKIAGIKMCQAYRRQYGCDF
                     IAAMPTNLYGPNDNFDLQTAHVLAALLRRFHEAREQGTPPTLWGTGAPRREFLHVDDC
                     ADACLFLMDCYSDAMIMNVGAGQDIAIAELAALVAEVVGYRGDMQWDRAKPDGMPRKL
                     MDSSRIAALGWKPATSLADGLRRTYGWYRQQLGESRSGVS"
     gene            18705..20603
                     /gene="asnB"
                     /locus_tag="PK224_09395"
     CDS             18705..20603
                     /gene="asnB"
                     /locus_tag="PK224_09395"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB15991.1"
                     /translation="MCGVVGALVLSPGSFRISEPYLMRMRDSMAHRGPDGAGLWIDSH
                     ARIGLGHRRLSIIDLSEAARQPMANEDETLWVAFNGEIYNHIEIRRELEALGGHRWRT
                     DHSDTEVILHAFETWGIDCVDKFRGMFAIALWDVRQQELWLIRDRIGVKPLYYSLHHG
                     RLVFASEIKALLQDPEQPRALHEESLFHYLSFLTTPAPQTLFDGIKKLPGGTWLRVRA
                     DGTMEERRYWDVWDHTSPLLGETDEAIAARVLEKLRESVALRKVSDVPVGVFLSGGVD
                     SSTNAALFSQGDTQRVRTFTIGYDGDYPSYRNELSHARSMAEQVGADHHERLLSLDDL
                     IEFLPRMVALQDEPIADPVCVPVFYVSELARRHGVIVCQVGEGADELFWGYPSWKTSK
                     MLEEWNHWPVPAVLKRLGLHGLQWCGYGESIHYERLRRGILHQPVFWGGAEAFPEALK
                     QRLLSPRLQRQFRHLTSWEAIRPIHDRFRAKAWEQGPLQWMSYLDLNFRLPELLLMRV
                     DKMSMGASLEARVPFLDHEFVQLAMSVPEAVKTRGGVVKTLLKQAVRGVIPDAIIDRP
                     KQGFGVPVQEWMQGRLGTLMQDTLADFCDRTDILNKDAVLDLVCRQRDPRSWYLFNLA
                     LWWKAYLV"
     gene            20699..22822
                     /locus_tag="PK224_09400"
     CDS             20699..22822
                     /locus_tag="PK224_09400"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRB15992.1"
                     /translation="MANAEDLDFATRLLSKAQHIGWLATARMGFRKVLRPWLSRRHRA
                     HVLRQPYRVDRLDLAAAFGVTPEDLTAAVERVRLALPQRLPVSPESVAEIRALYKKQA
                     PGVLEATVESADRICGHLFDLLGSGPVALGATIDWHRDFKSGYRWNPDQCFLDVAHGH
                     AVGVDIKVPWELSRGHHLVLLAQTALLSGDEKYARECLAQMTSWIDANPTAHGVNWAC
                     PMDVAFRAVNWLWSLGLLAESPLMTEAWLTEVLASLVAHGRFLMSNLEVRDDGVTTNH
                     YLADLVGLLYLGLCLKEVRDAEGWKAFAVRELVREMDRQVLADGAHYESSLSYHRLVT
                     EMFLSSAMLCRRHGVELPPSFYDRLRKMCEFVQGYTKPNGLAPQIGDADNGRLHILTG
                     YGQADVRDHRHLLAVGAVLFDRPDWWAAAGLTWVEGLWFGGLRCAAWGRAQGSAQSSA
                     QSGSRAFPEAGLYILRRQADYALLNCNPIGSRGVGTHKHNDLLAVEMHLGGEDILVDS
                     GCFLYTSDPQSYNRFRSTLYHSTVRVDQAEQNRLIPGKFFCLHPDSRLHRFQWDIGGP
                     VEQAAADHDGYERFSHPVRHRRELWADWLNGSWRVTDHVFSPVSGSHPHRLEWTLTFA
                     PHCSLQPAEHGWSVVTPRQRFWLDGPRLSANGTPVAISPYLDEGTVAEQYGRARRAPF
                     LRWSWEGSLPVEGVFTIASLESRSA"
     gene            22850..23392
                     /gene="cysC"
                     /locus_tag="PK224_09405"
     CDS             22850..23392
                     /gene="cysC"
                     /locus_tag="PK224_09405"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRB15993.1"
                     /translation="MKKALTIWLTGLPCAGKTTLANMLFRSLLEQGLTNVEVLDGDVV
                     RTYLSKGLGFSRADRDTNILRIGWVCQVLTKHGVCSIVAAVSPFRAVRNEVRAMIEKV
                     GGPGSFIEVWVRCGVEECIRRDVKGMYAKALRGEIQQFTGVSDPYEEPLQADIVVDTS
                     RESPSVSVGRILEVVNRGNL"
     gene            23437..24858
                     /locus_tag="PK224_09410"
     CDS             23437..24858
                     /locus_tag="PK224_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRB15994.1"
                     /translation="MDRSFLWFLLPTLLQTLIGVGVMVPITTYYLDPADLGTVAILTA
                     LAMLVTPLASTGDNWVLSTHWHATPDSGRKELLFNLLLANLGMKTLWVAVFWLLAPVV
                     LPQLIRDYRPEYQQYFGLVLLGLLAGTTWGTLSPLMVIERAPVSHAMNESLQWAAGAT
                     MTLVGLSVLKLGVLAMFLAPIAAGLASVLHGFGYAARRVTLRPSLHWSKEIVRSGLPA
                     IPFSLMDVLANSMDRFVIQRWLDLSTLGIYAHSQSYRGMFVTVTKAYSRTMTPTFLEL
                     FAGNPRQSQRQVEETVSLWYLCVTAGGILVTLFSPEVIHLLTHGKFDQAAALVPIWFL
                     LVCAHSMAIPFTQYLLVVRQNVLLSWSSIAMSLGTIGLVCLATWQFGVIGATGAAVLG
                     AMALQLMRFVLARRFGCPYGIESGMLWGMGLLLVLYGVTTRIELSLPVKALLALTVIG
                     VTIRQIVRRISVRDLLTALVPSK"
     gene            24907..25983
                     /locus_tag="PK224_09415"
     CDS             24907..25983
                     /locus_tag="PK224_09415"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB15995.1"
                     /translation="MGKRVLITGGAGFVGSSLALMFREAYPDWEIVAFDNLRRRGSEL
                     NLDRLRRCGIRFCHGDVRNRSDLDGVGKVDTIIECSAEPSALAGFDGEAAYVVDTNLA
                     GTVNCLELARRYQAELVFLSTSRVYPYEALNTLPFLETPTRFDWQLDRACTGVSAQGV
                     AEDFPLEGVRTLYGATKLCAELLIREYVSMYAVRAVLNRCGVLTGPWQMGKVDQGFAV
                     LWAARHHFGGALSYIGFGGQGKQVRDLLHVEDLFELLRLQLRDLAVHSGQVYNVGGGR
                     DVSVSLLELTGLCRAATGKTIPIHSEASTRPGDVRVYLTDSSKVRAAAGWKPRWTVER
                     IVESIVAWIRERENELRPVLGENR"
     gene            26028..27341
                     /locus_tag="PK224_09420"
     CDS             26028..27341
                     /locus_tag="PK224_09420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB15996.1"
                     /translation="MRVGFIVQGLPGEGYHGGALTCWSVVRELIRSGHQVCVISLFDV
                     TEGNPYLKSRGQQEAALRREGAEVVVIEYAMEALTGARSHDPLAKFGRWIGRICSAPD
                     GLAYLMPWVKLAEEVRRALAGREFDGFLCYHFDALAAVHPLCLSPMLAVVGDLWHLPG
                     YFRWREGSFSLSKYAVRGIRVALESMIAERAMSTLLASTTSCGAFAGHYAEWLKAHGF
                     PKTLYFRTPVPDAAGPKWDLLRSEARAGRTGGKHRVLVIGDLATTSTSSGLRAVAHAV
                     LPRLVDEFGEDGFELRLVGGGAPPNDIAPALRKPYVCFAGRVVPADPEFLAADVMIVP
                     TNIPLGIRVRVITAWSFGCPVVAHRANASGIPEMRHGENSLLADTDAQLVDAVLRVLT
                     DHTLRSGLSAGGRGTFERSFSESVAGQQIVKEMERLAGAQATNRS"
     gene            27400..28182
                     /locus_tag="PK224_09425"
     CDS             27400..28182
                     /locus_tag="PK224_09425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB15997.1"
                     /translation="MKNGLSARIGNGLAAALPENICAAFAKRLADRNPQLLLEALGPR
                     LNRTPSLDTMYFDLAPTGTLQFEDLAGLFASTSLDHGVIAMTVRQTAYLFGLVRRMKA
                     RRVIEIGRYKGGSTLTIAAAMSGEGTFWSIDLGEKEARLHRRGTSRTFDDEIRDICAR
                     FGLSVTLLVGDSRTIEVETGEVDLVFIDGDHSYEGVKNDFERFGRRVKVGGAVLFDDA
                     CDEAVFHTHSESVGKLLDEIVAQQSFRLVKSVNRLAHIERVR"
     gene            28193..29320
                     /locus_tag="PK224_09430"
     CDS             28193..29320
                     /locus_tag="PK224_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRB15998.1"
                     /translation="MLVGIFGAGRNGSSLLMRLLDGSPGLWIYPIELNYLRSFAPRSL
                     KSAVKRTVASCASHLSGQAGETFEQRQRQLVRAWAADQLDELKQTYLDKLVEPISPDG
                     VPLERIMAHITGDATHDLESYLDAIRLCYDRRTLPTPPLLMFKSIEVSDLFRYQAMFP
                     GMKCLHIMRHPYSNYSSLKRTDMVLKQKPFWFQGGDILRLQLESRWIPHAEFTLQGLT
                     TDPTRHYLVRYEDICDSPTRTVTDICSWLGVAPPDEPTVQTVLGGRHTKSLPINSSLK
                     GVETPAEVVSDMAKAYGYDDILTERERNLILLRTYQLGRRLGYFSEQDAAQVPARLPL
                     FLQWLAPDQWEYMNASSKVRLARALIQRRLYLCRVLLSPLA"
     gene            29339..30385
                     /locus_tag="PK224_09435"
     CDS             29339..30385
                     /locus_tag="PK224_09435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar O-methyltransferase"
                     /protein_id="HRB15999.1"
                     /translation="MKFAVRDKTMFQTAFSPTREEVRTLLDYVRFLWEEEVGARIAAH
                     PLYRPSDLWKHWAGQTFTGFSLDTILLQGGHAFPSSVEVGLGGEGGETTSRWLRCLGA
                     LFGGKEAARIRQGRDPVQLQRRFREFYPHVAVCPEHERWYAWHPNPYFRCLEAYVLRE
                     KSPLPEQMLEVGAGACVNVAFYRSLNPSLRTTIIDLPETIMFGYCFLKSVFPDIRIAL
                     PHEVGSRQADERADVRFLLPTQVDWVPDDSMDFCFNMSSFQEMTIDTVNHYLRFMART
                     LKPGGTLLSVNLETSRYLEGNALNHFDFSLYQATPRVQPAPFGTDLVGHIPGLRMAHA
                     EVVNSARTPEAVRR"
     gene            30385..31077
                     /locus_tag="PK224_09440"
     CDS             30385..31077
                     /locus_tag="PK224_09440"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HRB16000.1"
                     /translation="MHVLAVIPARGGSKSIPLKNIRPLNGTPLLVFTIQAAKRSAYLD
                     RCVVSTDHVEIARVARDYGAEVIERPADLATDQAPTEWALLHALEVLAKDGYRPEYVV
                     TLEPTSPLRTTGLIDRCIAEAIRHPELDCVMTVAETRKCYGRLQDGRFEYLFPDQPRR
                     RQERQPLYEESSTVYVTRTEVLERDRSVLGRTQYGVVVEDPREAIDINEPLDFIVAEA
                     VLAQRLVEEQHG"
     gene            31070..32116
                     /locus_tag="PK224_09445"
     CDS             31070..32116
                     /locus_tag="PK224_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRB16001.1"
                     /translation="MADIESIPIGPYRIGPDHSPLVIAEAAVNHQGDFETAKRMVYIA
                     HAMGAHIIKFQIHVLDNEMLRETPQSANFAEPLYVTLDKTNLSIDQHRELKRLCESLG
                     ILYLCTPFSRIGADMLEELGVAAYKTGSGELTNLPLIEHIGRKGKPMIVSTGMCTIEE
                     VGETVALLRQLKTPFALTHCVSAYPTPYDRVNLGMIARYRELFSVPVGLSDHSRGIYT
                     GIGAAALGACLIEKHFTLDRQQPGPDHPVSIEPDELSELVKGVDAVFRGRGAERKIFP
                     EEREIVAWARESVVSEVAIPRGTVITPTMVWVKRPSPGPGAVAAKDLKKVIGKVAKTD
                     IPKDSQIRWEQLNA"
     gene            32113..33264
                     /gene="neuC"
                     /locus_tag="PK224_09450"
     CDS             32113..33264
                     /gene="neuC"
                     /locus_tag="PK224_09450"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRB16002.1"
                     /translation="MKKRRIAVVSEARATYGYVKGVMHLIEQSERLELQLIVTGMHLL
                     KEYGSSINEIVRDGFTPAARVDMYVGGDTPTAWSKSLGVEMQGLAQVFDMLKPDLLLV
                     AGDRAEILAATVTAAYMNIPVAHIQSGDLSGHIDGSARHAITKLAHIHLPACEDSAER
                     VRKMGEEPWRIFNVGAPQLDDVVHGKKLDRNELAKVFGVDFDKPVLLVIQHAVLAEVH
                     LARKQMEETLAAVKESGHQALVVYPNVDAAGQEIIGVIRQYEQVPTIKTFRNLDREVF
                     LSLLTAVSVLIGNSSVGILEAPSFKLPALDIGSRQTGRMRACNVITVPEFDRRLIGQA
                     IERALHDSQFRATLKTCENPYGDGDSSERICKVLEDVDLGKLLNKQMTY"
     gene            33278..34276
                     /locus_tag="PK224_09455"
     CDS             33278..34276
                     /locus_tag="PK224_09455"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRB16003.1"
                     /translation="MNAPVLVTGGAGCIGIQVCRELDRRGIEVHLLDLGEQIARVRKA
                     LPEKAKVFYGSILDVSSIREAMAGCGAVIHLAALLGVRRTEVNRLRCLEINVDGTKRV
                     LDCAIQHRIKRLVFASSSEVYGEPIENPITEETITQGKTVYAVSKLAGEELCIGYAQR
                     YPEFEHVILRFFNAYGPYQAAQFVLPKFIQNAMTRKPIVINGSGDQIRSYCYSEDTAR
                     GVVEALLRPEAVGQVINLGNSDRPTSLKELADLVVKASGNPSVEIKYAADFQGTDRHA
                     SREIHRRYCSGEKAKRLLGFESRVTLEDGIRRIIEMNSIFEKWESTELPYLIDEMT"
     gene            34307..35995
                     /locus_tag="PK224_09460"
     CDS             34307..35995
                     /locus_tag="PK224_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16004.1"
                     /translation="MTHLNWKTSLKRALSRAHVLSAVRSLHYQFRRTVDRDFRNQESV
                     REQEFREFSSEYGAAFRHLLPEQHGLARQVLIVSSGSLGLLVEIALVKAFQAAGYHPV
                     VLTDYDRWVDAFYREAGIQDLLCWDEFVRLSPDAEAVRIMADVASFEQLLALDYGGIR
                     VGKYAASTALRHLRVGRLDLTNQAVRDALLPFLQRSLSYAKAAQNIVKTVRPQLVLSV
                     DLGYSPRGELFDVCLAAGLETITWNAAHKNNQLMLKRYSRANRDVHPASLSVRTWHDL
                     QAQPWTHAERERLRRELVGSYLSGEWYGEVGTQFHTTLQEAAEIRRALDLDDRKKTAV
                     IFSHIFWDGTFFYGTDLFGSYEEWFVETMKVACANPHLNWIVKIHPANIVKNVRDGVG
                     SEPSELTAIRSLGETLPAHVRVVQPDSHMSTLSLYSVIDYCVTVRGTVGIEAACFGIP
                     VLTAGTGRYDRLGFTIDSDSRAEYLNRLAHIQEIPSQSAAQRVLAERFAHGVFVLRPF
                     SLHTVTLEYQRDAKASLRTRVCLPAGGDLRAATDLRTLADWINSGQEDFLAPSL"
     gene            36043..37998
                     /gene="asnB"
                     /locus_tag="PK224_09465"
     CDS             36043..37998
                     /gene="asnB"
                     /locus_tag="PK224_09465"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB16005.1"
                     /translation="MCGIAGMVGTGASERVRSMTDALVHRGPDDGGYYDDPPSHLCLG
                     MRRLSILDLEHGHQPMANRDQSVWIVYNGEIFNSPELRRQLEQRQHRFVTANSDTETL
                     LNLYDEKQENMLADLNGMFAFVLHDRKRNLLFGARDRLGIKPLYYARTPAAFGFASEL
                     KSLLRMSGLTRALDENSFSHYLTLRFVPGTSSMVKGVSRLAPGHWFRYELETGRLETQ
                     CYWRPSFAPQEGRSAAEWSELVRAELRAAVQRWSLSDVPIGCSLSGGLDSSSIVGLLA
                     ESGHRQLKTYSLGFVGEGAAALDELPLARTVAERWGTDHHELVLQPDDLLRDLLKMVW
                     YLDEPYGGGLPSWYVFQFMQRDVKVGMTGTGGDELFGDYGRYIALERRTVGRPAGAST
                     VPAWMERCWPLVSSLIDRLPRRVGGVRSRERWRNWPMVGRDPFKWDYVHAFYYFSDAM
                     KRTDVLADLAEGKPDQDTAEMLRDYYRQSDSAEPRNALMQLAMRTQLSEEFLFMTDRF
                     SMAHSLEARVPFLDHTFVEAVMRIPSHIRTKPGDPKYLLRQAMKDLLPSELLHAPKRG
                     FVIPTAQWLRGPLRPLAERLLAPKRLADQGLFRPEFYARFVQPHLEGQADYHPQVWTA
                     LMFQVWHSLYLELALTDCPNFSWQELC"
     gene            38087..39088
                     /locus_tag="PK224_09470"
     CDS             38087..39088
                     /locus_tag="PK224_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB16006.1"
                     /translation="MRREHISELVCAGGQHRLNLQEGAFEEQGDIKEGALTCVATCPT
                     VPIRGSVPRFVPDSGYSQNFGEQWNHFRRTQIDKFNGTDLSKERFYSGTGWSPAELKG
                     AKILEAGCGAGRFTQVMLDAGAQVYSVDLSSAVDACFANNGPRENLCVVQADLCRIPF
                     RHHSFDKVFCYGVLQHTPDPHASFMSLIPFLKPGGDLAVDVYLKGWALEPYKSKYLYR
                     PLTTRMPRHLLFRFLQWYIPKWLPVDTFIKRLPLVGRVLGMLIPCWNYHYLPLSQQQK
                     TEWGILDTFDALAPAYDYPQTPETVTEWFTSAGLMDIRVRLGGNGVLGNGRTRPFPV"
     gene            39098..40333
                     /locus_tag="PK224_09475"
     CDS             39098..40333
                     /locus_tag="PK224_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB16007.1"
                     /translation="MNILHLSTEYNLGGSGRAAYRIHSSLLRRGHTSRMLVSGLVHGV
                     PEAGPIWGNMPWRAADWLARRATDALSLQYLFLPSSWRLLRHPWFQAADVVQLYNTHG
                     GYFSHSVLGVASRRKPFVWRLSDMWPLTGHCCYSYNCDRWKTGCGACPLLHDEPALKA
                     DRTALLWRTKQRIYAGANVTLVAPSRWIAGLTEQSPLVGHWPVQVIPNGLDLRVFRPI
                     EQAAAREVLGLPQGEDLVLFSSVETQAYRKGGTFVAEAVNAIKGKTRKPFRLLVVGNR
                     ASEWETAVSVPVTAMETVKDDYLLAVLYSAADLFVHPALADNLPNGVLESLACGTPVV
                     AFDVGGVGEAVRPMETGYLARDKDTGDLSEGIKRLLDDPDLRRRLRTRCREVAETEYD
                     MALQTRRFEELYETVIHGR"
     gene            40323..41168
                     /locus_tag="PK224_09480"
     CDS             40323..41168
                     /locus_tag="PK224_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB16008.1"
                     /translation="MADSRAENFSETSYGIVKRLQTIEAWTDRVAQRLGKPSLSILDF
                     GCGTGDHVTYPLACLGHRVLGVDVHAASIQEASGKYRLPTLRFRRAEIDDLLKEDVRF
                     DLIVCSEVLEHLREPQPFLEALGRLLHDRGALILTTPNGYGAFEWLTSLQKALSRVGL
                     HEFFRQVARLLLGKQALSPAGRGADAGHVPVCSAGYLNMDSTHVQFFRIAGLEAILAQ
                     SGFRIVERRARTSFCGPYVDVLFRVLPFEQALCRMNNRLADLLPFRWAADWMFLVEPQ
                     GKSPA"
     gene            41165..42466
                     /locus_tag="PK224_09485"
     CDS             41165..42466
                     /locus_tag="PK224_09485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16009.1"
                     /translation="MSVELPRVLVVTANNFNLVSGGGITLTNLFRGWPHDRLANVHED
                     AVPEDHSVCRTFYRLTEEEIRWNWPFSLLQGWYGRKKQQAISTGGSAPGPAGQGWLSA
                     VKRLLGDGAPKQARLTPALLDWVEKFHPTLLYGFLGSLEQITLIRSLAARFNIPMVVH
                     MMDDWPAVLHRRGLLSPIMGPLIRSELACALKEARGRLAICDDMCTEYRDRYGYEFTA
                     FQNALDPAGWLPHARHQWQAGVPFVVRYVGSILADGQQESLKDIALAVTGLRRAGRPI
                     QLWIHAPGPDADYLRRLGMPADAVVIEGPPDPVLVPRLLAESDLLLLPYNFDVRSARY
                     IRLSLPTKAPAYMISATPVLVYAPSDVATVRYAAREGWGYVVSSQGEQAVAEALTLLM
                     DDQTLRERLGRKAQQVALLRHDAGRVRQAFWETLTAAARVS"
     gene            42551..43372
                     /locus_tag="PK224_09490"
     CDS             42551..43372
                     /locus_tag="PK224_09490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB16010.1"
                     /translation="MRKGRQVPVMTEYGADSQTVSSQNEDVRQYWDQEACGTYKSVVG
                     ALPELSREWFASVEQHRYAMEPFIHTIAEFPTHRGKTVLEVGVGAGTDHLQWARAGAV
                     CFGVDLTQRAIDTTRAHLTLHGFSSTLQRIDAETLPFPDASFDVVYSWGVIHHSARPE
                     QIVREIRRVLKPEGMFIGMLYARHSLVVFKLWVKHALLKGRPWRSWADLVAHHMESPG
                     TKAYTVPELNALWSEFSSCETTQILTPYDYNWFPGWMRRWFRDRWGFFIAVRARK"
     gene            43414..44571
                     /locus_tag="PK224_09495"
     CDS             43414..44571
                     /locus_tag="PK224_09495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16011.1"
                     /translation="MRPKRLLMASANYWRSPFQVGSHHLARGFVRAGWEVAFVSDPIS
                     PWHLFGGQLKDLRERYELYRGNGVTDCDGHLWAYVPGALLTPHNKPLLRSRWTGERWA
                     SLSLPSLMDRLWVRGFDEVDLLYCDSVAHVGWIKDIVRRKSVYRVTDCLAGFQKTTPV
                     ALELEKELAGSVDLVVYAARSLEDYVKQLMPKSMAYLPNAVNYAHFGQQSCARPPEYD
                     AIPGPIALYVGAMDVWFDYRLLDEAAARLPEISFVLIGPDALAKQRLRARPNLHLLGK
                     REYRELPRYLQHADVGLIPFDVANHSELVRSIHPLKLYEYFACGLPVVAVEWEELTYL
                     RSPAHLCRGTGEFIAAIEQAIANPAETSTLQSYAAGHDWSERVKAILSHLG"
     gene            44730..47357
                     /locus_tag="PK224_09500"
     CDS             44730..47357
                     /locus_tag="PK224_09500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16012.1"
                     /translation="MTSTETHLSGPDRVGLGLLALFLFSPVMFKIQVSQGVAIHPCAP
                     LLAAAWGWIAWMIGRTQVTRSEKMWLIERQAWNIPMILSFLVIGGLALSLAINGLRLG
                     SFSSAGWLLLFKWALYLAPLPLAALLVMRTGRRVLQLAAWLIPAVAFLTLLYTAARFL
                     QAAGGEYFNAYFDGKAMFLAMGMMGEVLSAEGLTVRSDTVSHGAYGMYLVLVLAFSSS
                     LAWLRGWNGILPASYAVAQGIILGPFLVLGVLLTGSRSSLVLLAGMLLMFLTLLIANP
                     GHAQPPQRRIAAAALVLTVSACLFFLPRWFGPIFPTLERMHQTVDQPLELQATLLGRQ
                     TPDFDPGTQANVAVRNAQSRVWLWGQTFRYMTEHPLTLITGIGYDRRRFVEQVVGLPY
                     VGTNIELRTAHNLYLDVAIKGGIAPFVSLVLLCLWLLWTAWTSVWITTSAGPGAELYG
                     AGWVILTLWPPLLLVSGLGEDLVTDNLLLHWTTLFGLVLGLRGAILRSAMAGRIVHLS
                     ATAGLGGGPAYITAVARHQVHHATEVRVVCSDEKPYVDIWREMGVRVTVLPMRRPNAR
                     TIWTIVGLMLRDPAPVHAHGRGAAFFAFWIKMCVRVPVVYTPHGPHYAYMRGWRFVPA
                     WCGEYLFRIVFDAVLYVSSGERDLAESLRLPMRSSRVVLSGMISDPGHELEQPARREA
                     VRREVGVAPSQFVIGWIGRFHHQKGLDIFLASIPEVAARVPDAVWLVIGEGEPGAVER
                     HRETMRRLGLGEKVTFLGGRPDADQVAGVCDLYISTSRWEGLPLVLLEVMARDVPIVA
                     SDVVGNGDVLDGWGVLFPPNDAAAAAEAQVALATDPMKRQALARRGRAVLHERFLLTR
                     MLRDLDHVYRDVWGSEVLS"
     gene            47354..48706
                     /locus_tag="PK224_09505"
     CDS             47354..48706
                     /locus_tag="PK224_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16013.1"
                     /translation="MRAVADWYSTAGFLAKLGKTALPLVASDAVLQGCRYGLVAYLGI
                     LSLSLLGSYLFGSAIGALAAVAIDFGINQHWLRLDSVAGGLTRQAFTRVLAVKLVLSL
                     ILVAGFVLLMEGGFWSRPFPAMVVTGLIMANLHALVETCESVGFLFRRHHLVALVRVV
                     LGLMMYAMPAAAALFMGRPSGEVMIQPLLSAGLLAGLLILSGYVWKTSRMLVDGPESE
                     QGYLRTWRASRWLGLNQLAIVVDVRAPLVLLGLLLGETAVGLYGFVQRTTAVVELAWA
                     SISKLLLKSYAEDASLKGGRVVQQHVRRASWVTGTAMIVGIAGVWIGVHYIERLDRWS
                     EDVRTGLALLRWAAVAIGLSSLKRPLVLGLIALHHEREVCTVNVLSAAAGLTFIPFLI
                     WSLSIWGPLVAAVGCETGAIVLLAIYFRAALRRLEAETDHVRNSRWVSGEPARYDRSI
                     "
     gene            48703..49878
                     /locus_tag="PK224_09510"
     CDS             48703..49878
                     /locus_tag="PK224_09510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16014.1"
                     /translation="MKVAIVHDWLTGMRGGERCLEAFCELFPDADLYTLLHIKGTVSP
                     TIERHRITTSFVQSFPLAARYYRYYLPVFPFAIERFRLPSYDLVLSSSHCVAKGIRPP
                     AGGKHLCYIHSPMRYVWDQFDNYARGGRSGVVARLGMELFRKRLQAWDVASAARVDQF
                     VANSHNIAGKVQRYYNRPAAVLHPPVDWESFQAAEQSEDFYLMVTAFAPYKRVDLAIQ
                     ACNVMKRRLKIIGKGQEEARLRKLAGPTVEFLGWQPDDVVRDHYARCRALLFPGEEDF
                     GIVPLEAMACGKPVLAFGRGGALETVVPLPGAGSAEGGPGQGGSGSVTSAGKVATGVF
                     FFTQAVESVVDAMECFERRRTEFDPHAIRDHVAAFDRRLFKERVKAFAMGALTGTAS"
     gene            49886..51286
                     /locus_tag="PK224_09515"
     CDS             49886..51286
                     /locus_tag="PK224_09515"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRB16015.1"
                     /translation="MLKRHSQFLKSLLFCIDLGLICACWVGAYYLRFSEIVEPATKGI
                     PPLRIYLLLLVPIIAVWGMSFQAFDLYRPRRMGTRLSEFLDVAKANTLSVLILVALTF
                     FFRQYEYSRLVLLYFWLLNLITLGFSRVLFREALRFLRRHGYNQRHALVIGTSRLGRR
                     VVDALAQHPELGVKVHGFLSADSRKVGERINGVPVIGRYDDLEERVQSGVDIVFVCLP
                     PEDEQWAEKMFACLATTMVEVKALPAMCEFVSLRAEAEMFEGLPLITLQGSPLYGWNL
                     VIKRVLDVVGASAALVVFSPVLCAIAVLVKLTSPGPVFFRQLRMGLDGQAFEMLKFRS
                     MKLNAESETGPVWTQPNDDRRTPIGAFLRRTSLDELPQFWNVLRGEMSIVGPRPERPE
                     FIARFRETLPQYMLRHKMKAGITGWAQINGWRGNTSLERRIEHDLYYIEHWSIWFDIK
                     IMLLTAWRGFIHRHAY"
     gene            51423..51962
                     /locus_tag="PK224_09520"
     CDS             51423..51962
                     /locus_tag="PK224_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRB16016.1"
                     /translation="MPRTTRRTKSPAKPRFSLQGTLRGIRLFATDVDGVLTDAGMYYS
                     ESGDEWKKFNTRDGMGIKLLQKAGIVTAIITQESTKIVMRRAQKLTIPEVHQGVYDKL
                     AVLKELIARHGFSMDQVAYIGDDVNDLQALGAVGFSAAPSDGMPPVLKTVRYVCKKKG
                     GEGAVREVADLILAARQPR"
     gene            52004..53455
                     /locus_tag="PK224_09525"
     CDS             52004..53455
                     /locus_tag="PK224_09525"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HRB16017.1"
                     /translation="MDMTEHLYPVILAGGSGTRFWPLSRHLYPKQLLRIIGDETLIQQ
                     TMRRVLSCAKPEQVIISTNPGQADSIRVQLGEWKSALKNNYVVEPVGRNTAPAIALVA
                     AELLLRDPHAMMLVLPADHVVTGEKAFKAAVALGVQLAEQGHLVTFGIKPTRPETGYG
                     YIQPNRRKSLAKKGRLTGHPVARFVEKPNRTKAAQYLKNGHFYWNSGMFLWKAATILE
                     EIQQHQPQLAKAIQRVHALMASGAEPGLVEAAYKKVPSVSIDNGVMEQSANAAMIPVG
                     FGWSDVGNWSSLEEVAPRDKAGNVVSGRVIDMDSTNSVLYADRRVVATIGLSDMVVVD
                     TPDATLICPKSRSQDVKQMVEILKQQGAPEHLEHLTVFRPWGSYTVLEEGPGYKVKRV
                     TVNPGGRLSLQLHHKRSEHWVVIAGTARVTRGDELLDLRVGQSTAIPVETPHRLENLG
                     SETLHIIEVQNGPYLGEDDIVRFQDAYGRTSVR"
     gene            53537..>54339
                     /locus_tag="PK224_09530"
     CDS             53537..>54339
                     /locus_tag="PK224_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HRB16018.1"
                     /translation="MALFREYDLRGVVGEELTEAIAEQVGRAYATMAREQGASRISLG
                     RDGRLSSPGLQQALLRGLLAGGLDVVDLGLCASPLLYFSLFTLPVDGGIMITGSHNAA
                     EYNGFKICIGKEAIHGEEIQRLRRVMEAGQFTTGSGRLSSHPIIPDYLEHLKTNFSGV
                     RADHLHVVIDCGNGAASLVAKQALEQMGCRVTGLYDELDGRFPNHHPDPTVVENLQDL
                     IATVREKKADVGIGYDGDADRIGAIDERGEILWGDRLMIVYARDILTQRP"
BASE COUNT         9762 a        14909 c        17812 g        11856 t
ORIGIN      
        1 gattgagacg tctgacccag cccttcctcc tgtcgtttcg atctgggctg cacgtggggt
       61 gtgtgctgac cggcttgttg ttgtttgtcc tgctgtgtgt ggacatgggg tactacgggt
      121 tcaaccggca gcatatggac ttcgtgtttc tcgaatatgt cggcgacctc tttgcgcctg
      181 cggcgacatc ggacagcacc aatgtgcagg cgatgcagca aaccagcgca gagttaggcg
      241 cggcaggtaa atgggcggga aggctggcgc tctttctggg catccaggct gtggccatgg
      301 ggctgtggtg gtggagtttt tcctgcgcca ttgtgccggc gttggctcgc tggcggccgg
      361 gctcggggtt tcaggcgaac acgttcctcc ttgttgcgct ggtggcgggc ggcgccgggt
      421 tccatcattc cggcccctac gggattcgga tcgcgccgat cggaagcatg gtctattaca
      481 cgctggctca gaatcccgtc ctctttgcgg ctgaagccct gcgaatcgcg gtggtatcgc
      541 gagggtcgat ggagcaacgg gcaagtgccg aggcgatgcc ctatgaggtc gccgttcaga
      601 ccgtgcagcg attgctcggc ccgggtgaag catttgtgga tcagcggtat cccctggtca
      661 gaaccatggc cgcctcttct gatggtgttc ggttacccgg gcaggcgaac atcctgttgg
      721 tatttctcga agggctggat cgccggtatc tcgagcagaa gtacggcgag attcagggga
      781 cacctttttt ggatcgcctg aagcaggaga gcgtctactt cgagaacttt ttctccaacg
      841 gcgttcaaac gtcacggggg ctgtttgcga ccctctgcag cgcctttccg cggcaagggg
      901 ccgccgcgat gaaaacgcgc tatgcccacg attacctctg tctgccgtct ttgttacagc
      961 gtcagggcta ccgaacggag atggtgatcg ggcagcatcg ggacttgaac cggcttcagt
     1021 cgttcgtggc gcgcaatggt ttgcagcagc tgatcgacga gggagatttt cccaaggatg
     1081 cggagcggat gggcctcggc atcgtggacg gcgccctgtt cgatctctgt tacgaacgca
     1141 tcaagcaacg ccaggcggag gataaaccgt tttttctgac gaccctcacg ttgtcgaccc
     1201 accatccctt cgcggccccg cagaaccatc ccgacgtgcg tctgctgcag gagcaggtgc
     1261 aagacaagta tgtcggcgct ctgcgatata cggatctcga atttgagcgg gtgtttacca
     1321 gactcgttcg cgagggactc ttgcggaata cggttgtcgt cgtcctgggt gaccatggcc
     1381 ggcacgaacc ggtcgggagc acagatatgg agcgaaaggc gggtcatttt gcatcgccgc
     1441 tgtttatctg gatggacgag tcgttgcgca ctccggccac ctatcgcccg cggaccgtgt
     1501 cggccattgc cagccaggtc gatgtggctc cgaccctgct ggcgctcaac ggcattctac
     1561 ctgccgtggg ggcgttcgcc ggacatgatt tgacctgtct gttggtgcgg gattgtttgc
     1621 cgaaccaggt ggcctatctc accagtgtct acgacaatct ggtcggcctg gccagtccgg
     1681 acgggctgtt attgtattcg ttctcgaccg agtcgtttca agaggccacg ctcaatttcg
     1741 acgcggtatc cgacagcgag tcgggccggc gacgacggtt cgctactcac gacctggagt
     1801 tgatggccct ctacgaggtg gcgaatgtcg cgctcgattc gaatcgcctc tggtcctggc
     1861 gcgagttcgg gacacgatta tgatacgtgc ggggcacaat gtcttgttcg tcgttgttcg
     1921 ataaggacct gctgctgtga atcacattcc ggtgtcggcc gtggtcatcg cctacaatga
     1981 cgaacccaat atgcgggctt gtctggagtc catcacctgg gcggatgaaa tcatcgtagt
     2041 ggattctcac agtaccgacg ccaccgagcg tatcagccgg gaatttaccg accaggtcta
     2101 ccagcatgac tttcacggat tcggacgcct gcggaacgag gccctgaccc acgccacgca
     2161 cgattgggtg ttcagtctgg ataccgatga acgggcgacg ccggagttgc gcgacgagat
     2221 ccggcgcgtg ctggctgcag gcccgcaggc cgatgcctac ttcgtccccc gcaagaatta
     2281 ttttctcggg cgatggatca aacattgcgg ctggtttccc gactaccggc agccgcagtt
     2341 gttccggaag agccggctgc gttatcggga ggaactcgtc catgaaagtt tcgacctgga
     2401 cgggaaaatc ggctatttga cggccgctgc gctgcagtac ccgttccggg atatcgacca
     2461 ttacttagcg aagcaggagc gctattccga tctcatggca cggcgcatgg tcgaacaggg
     2521 gcgtgcattc tctccgcatc agctgatctc ccatccctgt ttcacctttc tcaaaatgta
     2581 cgtcgggcga aaaggttgtc tggacggtgt gccggggctt atcttgtccg gactctatgc
     2641 ctattatacg ttcatcaagt acgcgcgttt ctgggagttg cggtcccggg tgctgactga
     2701 gggcagccag actgcgccgg tgaagcaggg ctaagcatga cggttaccga cggtggagcg
     2761 gaacgcagaa ccatgacgat tggggcggta gtcatcacca aagatgaaga aaagaatatt
     2821 gccgactgtc tcgagtccct gcgctgggcc gacgaactga tcgtcgtgga tgcggaaagc
     2881 cgggaccgga cggtggagct cgcaaagcag tacaccccga acgtgtttgt ccgcccctgg
     2941 cccgggtacg gcccgcaaaa aaacttcggc atcgatcagg ccgccgcgga gtggattctg
     3001 gtggtggatg ccgacgagcg ggtcaccggg ggattgcgcc gggaaatcga agcattactg
     3061 gcgacggccc cggcggccga catcggaggg tatgagattc cccggcggaa ttttttctac
     3121 gggaaatgga ttcaaggtgg cgggctctat ccggactatc agctgcggct cttccgaaac
     3181 actgccggcc ggtacgacga tgtgaagctg catgaaaact tcacgctcag cgggcgtcgg
     3241 gagcggcttc aggagccctt cgatcactac agcatgccca ccgtcaatca tcacatccgc
     3301 aagatgatgc ggtatacgac tctcggtgcc gatgagaaac tcaagcgggt cacccatatc
     3361 agcggctggg tcattgccac gcaccatctt ggtacgattc tcaaaaccct gttctcacgc
     3421 ggcgggtatc gcgacggggt gcacggattg gtggtggcga tgtttgccgg cctccacaca
     3481 ttcgtcaaat atgccaaggc ctgggagcgt gtgaacgtga ggcgggacgc atgatcgatc
     3541 caaccttgcc cggcatgaac ctggcggagg tcccgttccc ctgatgcgta tcgggatcga
     3601 cgcagcgtca atcgtggggg acaagggcgg cgtcggctgg catacctatc atctgctccg
     3661 gtcgctgctg gccttggatg agcaagtcga gtatgtcgga tatctccggc ccgggtctct
     3721 gcaagcgggc cggctcgaag gctggccggt ccacgatcgt ctgcggtggg tggaaacgcc
     3781 gcggtggttg atgccatgcc ggggcgcgtg ggacaggctc gatctctatc acggccccaa
     3841 cttcaagatg catacgacgg gacgatcggg cggcatcgtc accatccatg acctgtggct
     3901 ggcccggcat ccggaatatt cgaggaagct gttggggcag gccggttcct cccggcgggc
     3961 catcgccacg gccaaccggg cgcgacgggt cgtgacggtg tcggagtttt cagcccgtga
     4021 aatagaagcc ttgtatggaa ttccgcgtga acgggtggtc gtcatccata acggcgtgac
     4081 cgaggaattc tctccggttc aggatgagca ggggatggca gtgttgaggg cacggtgggc
     4141 gattccatct gccggattta tcctgttcgt cggcggagca gatccacgaa aaaaccaccg
     4201 tgtttttctg caggccgtgg cacagtcgcg ttcgcagctt ggagggcggg ccattctgct
     4261 cgtgggagat gcggagcatc cgcagggaag ttatctcgcg acggctcggt cgctcgggtt
     4321 ggagcaggac gtgcgttgca ccggtcgcct caatcgggag gatctgtgcc ggctctactc
     4381 ttacgccgag gtgtttgtct ttccgtcgct ctatgagggc ttcggcatgc cggtgctgga
     4441 ggcgatggct tgtggagcgc cgacgattac ctcctcgacg tcgtctcttc cggaagtggc
     4501 aggggatgcg gcgttgctgg tgaacccaga ggatgccgag gcgttaggag cggcgatggt
     4561 gaaggtgctc tccgagcagg cgctgcgaca gcagcttcgg gatcgtggct ttgagcgggc
     4621 gcgactcttt acctggcaac gtgcggcgtt gcgcaccagc gccttgtatc gtgaactctg
     4681 tgcctgaggc aggctgttga aaaaaaccgc cagcatcgtt ccacttcgct cagaggttca
     4741 gcgtacggcc gagagtacgc tgaacctctg agcttgctgc ggccttgctg ggcggtcttt
     4801 ttgaacagcc tgcaggctat gagtgcccca tatgcgattc tcagtagttc tgaattccaa
     4861 aggtgttggg atagtgttgc agcgagctgc taagagcgac ggcgtgatgg atcttcagaa
     4921 tattctgatc atcaaattgc ggcatatcgg cgatgtgctc ctatctacgc cggtgttgcg
     4981 cggactgcgg actgcctttc cacgggcgcg cctgacgatg ctggtcaatc gtggaacgga
     5041 aggggtgttg gccaacaatc ccgacgtgaa tgaggtgttg tgtctcgaaa agggagcctg
     5101 ggacgcccag ctgaagtttg tgcagatgct gcgtcggcga ggctttgacg gcgtcgtcga
     5161 tctgaccgat ggcgatcgct cggccgtcat cagtctggcg accggtgcgc ccgtccgtat
     5221 cggatttaac gccgaacatc gctggcgcgg gctcttgtac agcaccgtgg ccaaaccacg
     5281 cccgacggat cagcatcgag tggactacga tctctgcgct ctgcgggccc tggggctgga
     5341 cacgaagccg ggcacaccgg cattgtatgc gtcgccggca gatgagcagg cggtggaggc
     5401 ctggctgcgg gaggccgggc tcttgtccgc gcaatcctct cagttgttga tattgttgca
     5461 accgggggcg cgctactcgc tgaaagtctg gccgcatgaa cggtttgccc agttggccga
     5521 tcgtctcgcc gacaggtttg cctgccgcat tctcctgggc ggcgaccagc gtgaacgcga
     5581 ggtggcggag caggtcgccc gcaagacgcg ctgtgcgcct atcgtggtcg cagggaagtt
     5641 ttcgctctta cagtttgccg cgttggtcaa acgttgtgcg ctgttcgtcg gaaacgacgg
     5701 tggcgccatg cacattgccg caacgatggg aacgccggtt gtggctattt tcggtccgac
     5761 ctatccgcaa cggtgggggc cgcgcggcgg accggcgcag gtgatttaca aaggcctgga
     5821 ttgccgggcc tgctatcatc cgacctgctt gcgcggggac gatagctgta tgcaacagat
     5881 tgcggtggat gaggtgttta cggcggccag ccggatgttg gaacgcacgc cggcgaggac
     5941 agagacatga gcgacgaact ggtcagcgtg gtcattccgg tgttcaacgg tgcgccattc
     6001 gtcgccaagg ccgtggccag cgtgcgggcg caggggcacg gtgccgtgga aatcctagta
     6061 gtggatgacg gatcgacaga cggaacgcag gaggtgttga accgtcttga gcaaagcgac
     6121 ggcattcgat ggtttcagcg cagccacggc ggaccggccc gttcccggaa ttacggcatc
     6181 gccgcagcgc agggacagtt catcgcgctg ttggattgtg acgatatctg gttgccgggc
     6241 aaactcgccg cccagttggc tatcatgcgt gcgcgaccgg acgtggggct ggttcatacg
     6301 gacttcgagg tacgatttga ggacggcacc ctggaggagc gcgtgtcggc acgatcgagc
     6361 cgtgagccga tggtgcaggc gtttgccggc ggtcatgttg cgctgccgtc gacactgctg
     6421 attcggaaat cagtgctcga tcagatcggg agtctggatc ctgaactgta cgggtcggaa
     6481 gactccgatc tcacgatccg gctgtttcgt gtgaccaggt tcgagtgcat cgatgaggtg
     6541 ttggtgcgca agctccagcg ggggcatggg tatcgagaca tggccttcga tgagcagacc
     6601 caccgggagc gggtgttggc cagccgtgac cgctttctga tgcgcctcga aagcttcgcg
     6661 ccgctcacgg cggtgcaacg cgccgcattg gaccgggaat gggcgaacta ctttctatta
     6721 aaaggggcgg cggcagaacg ggccggccgg cggagtgacg cgcggcggca ctattgggag
     6781 gcgatctgca aggccccggc ccgcgtgcga tgttatacgc gctggcttcg caccctgaag
     6841 ccctagcgac agcgccgggt atgccccaca ggccttgcgc cgagcacctc gcaaagagat
     6901 cgccaaagga tctatggaaa ccatcagttg cgacgtatgc ggagaggcct cgaacacagc
     6961 cctcttttgc caatatgatc tcacacatca cgtcaccgac gacctattta cggtcgttcg
     7021 ttgtcagggc tgccgcctgt tgttcctgaa tcctcgtccc actcgcgagg agatcggcgg
     7081 ctactatcct tccacctact atcccgaggc agcgcctcgt caggccggcg atctccgccg
     7141 cactgccaaa cgatggtcgg gaaaaatccg gcgatggatc gcccaggatt tttacggtta
     7201 cccggcgccg gaatcggccc gtcgctggca atgggcgcgc cggctcctgt tatggcctga
     7261 gttcctgtgg cgccgttggc gggggcgcgg gctgctcccc tgggttgggc aggggcgggt
     7321 gttggatgtc gggtgcggtt cgggaggcaa tctggcggtc ctgcaagagc aaggatggaa
     7381 cgtgtcgggg gtggatgcca gtgccgtggc agtggcacag gcgcaagctc gtttcggtga
     7441 gcgagtccgg cagggtgatc tggcgtcgat ggcctatccg gaacggacgt tcgatacggt
     7501 cctgtttagt catgtcctgg agcatttaca cgatccgctg tccctactca aagaggtctg
     7561 gcgaatattg gattgggagg ggcgggtcgt gatcctctgt ccgaatgccg gcagcctgga
     7621 agcgagaatg tttggacgat ggtggtttcc atgggaactg ccacgccatc tgtttcacta
     7681 tgaacgcgcc acgctgacgc ggctgttgga ggctgccggg ttccagattg agtcggtcag
     7741 caccggtttg ggatcgctct actttatggc gagcctggac cggttgtgtg aagagcggtt
     7801 caagcgagcg gtgccctggc gccggctgat cgagaaagtg ctcatccgcc cgttttgttt
     7861 ctgcgtcggc catctagggc atgggactga gttgaaagtg tatgccagga aaggtcggag
     7921 cgcgggctag acaaaatacg atctgaccag acgcgaccat gttttgacat tgtagggatc
     7981 gtggcggagg gctgactgga acgcctgacg agcggcggtg ggcgtgttgg cccgcaggtg
     8041 gtgtttgccg aggtcggcat aatagatcgc ccaatcgtgc gagagggcat cgccttcggg
     8101 ggtgccgggt cggcaagagg cggcgtgttt gtcgaggaac cggcgccgac cgtccagcgc
     8161 gcgcaacgag gggatattcc gcgacgcatt gtgtccgtag agccggtgcc gatagagcgg
     8221 ttttggaaga tgaatgaatc ggcccggacc gtagagccgg atgatcagat cgatgtcctc
     8281 ccaaatcgaa agtgccgcat cgtagccgcc cacggcatcg aaacaggtct tgcggaaaag
     8341 cgagcccgat gtggcggcgt cgtgtccgcc ccgcagcaat tgcgtgatgc tgcgctgctg
     8401 tttcatcgcg gcatcgtatt cgagaatctc gttccgttca ttgacctggt acccgtcgca
     8461 gtgcacgagg gttgcctgcg gagcgctctc gaggatgccg agcgtcgctt ccaggaaggt
     8521 gggctcccac agatcgtcct gatcgagaaa tgccagatag ggggtttggg ccaggcgcgc
     8581 gccggtattt ctggcggcgg cttgtccgct gttctgtggg tgacgcacgt agcggacctg
     8641 cgggatggct tgcaccaacg cgcctgtgcc gtccgtcgag gcatcgtcta cgacgatgat
     8701 ctcaccggcg gccagggttt gtgcgcgcac ggaatcgatg gcctccttca gatatctgga
     8761 ggtgccgttg taggcgggaa taacgacgct gatctgtgcc acgggcctgg tttccttgga
     8821 ggcgtcaatc ggtaacatag gcagtgaacc cagtcaaccg gcgcggctgc cgacccatcc
     8881 agacgacatc gattcgactg tcttgacttg ccgggtaggc tgtgattcca tgcgcgccga
     8941 ttgcgtgcgg gtggttgttc gtgatgccat cgcgcccaat cgttcgtgtg atggcccctg
     9001 tgcgtgatcg cgagccggtc ctgtcctcca ccataggtct cttccgcgca atattgtgaa
     9061 gatcgtcata gccgcatggc atctcaagaa tctcaacgtc gggatcgggc gatatgcccg
     9121 ggaactgatt gaggcgctgg ggcgcgttga tcagacgaat cactacgaga ttctgattcc
     9181 tcacgcggag catccgttca ccgcgcgtcc gaatatgcga tatcgcgtga tccggttccc
     9241 gctgtttcgt cgccgtttct gggagcaggt ggctccgctc ctgctcggcc cgtacgacgt
     9301 actccacttt ccgtacgatt cctgcgtggc ttggaaacga ggaaagttcg tggccaccat
     9361 ccatgacgtc aagccgttgc tgtttccggt gctccgtgcc cgtaccaatc tcaatagtcg
     9421 catcgaacag tggctgatcg gagatcgctg gaagaagatc gaccacgtca tcacgatctc
     9481 cgaaaattcg cggcgcgatc ttcttgcgca cgttccgctc cgccccgagc aggtgaccgt
     9541 gacgtcactg ggcctcgata ccgaacgatt tcgacccgcc gagcatcggg gggagggcaa
     9601 accctatgtc ttctgtgtgg ccggagccga cccgaccaag aacgtcggcg tcctcatcga
     9661 cgcgttcgcg aaacttcctg agacgctgcg cagccggttc gatctgctcc tggctggtga
     9721 tgtctgcaaa cgcccggata ttcgcgcggc cgtcgagcac catggcctcg gcgcccacac
     9781 gaaactggtc ggcatggtgt ctgaccggga acttgtcgcc tattatcagc aggccacggt
     9841 gtttgtgttt ccttcgctgt acgaaggctt cgggctgccg gtgttggagg ccatggggtg
     9901 cggctgtccg gtgatctgct cgaacgcgtc ctccctgcct gaagtggccg gcgatgcggc
     9961 ggtgctattc gatccgcgtg acggcggtcg attggccctt gaactctccc gtgtcctggc
    10021 gtcaccagcg ctgcgagatg aattgagggc ccgcggagtg gctcgggcgc gcgcattctc
    10081 ctgggatcgc accgcgattg agacggtggc ggtctatcga cgtctggcag gctgagtcag
    10141 tgaccctcga tgggcgagcc gtatcggcag caacacggat ggtgctcgac aggttgcagc
    10201 tcctgtgttg tcatgggtct cagaccggtt cttgcccgat gtcgagggga tgcacgatat
    10261 ggcggaagag acgtgcgatg ttttaatcgc cggggccgga gtgatcggcg tggccatcgc
    10321 gagggagctc aggcgtcgat atggcgtcaa ggtggtcgtg ctggagaagg aatccacggc
    10381 cggccggcat gcgagcggtc gcaacagcgg ggtgctgcac gcgggcgtgt attacaaggc
    10441 ggggacactc aaggctcggt tgtgtgtgga ggggaaccgg cggttgcggg cctattgtcg
    10501 gcagcaggcc attccgctca acgagcatgg caaggtgatt gtggcgcgga cggaagcgga
    10561 gttgccggcc ctgcgtgaat tgtatgcacg atcccaggcc aatggagttc gtgcgagcct
    10621 gctcgatcaa cagacgctca aagaggtgga accttgcgcg aagaccgtac agcaggccct
    10681 gtatgtgcac gataccgcgg tggtcaaccc tcggctggtc atggccgcca tggtggcgga
    10741 tgccgagcgc gagggcgtag agtttcgttt cggctgcgcc tggcaagggc gggacggcga
    10801 tggggtggcg cgcaccgatc gaggccgtgt gacctacgga catttcgtga actgcggcgg
    10861 actctttgcc gaccgaattg cgcatgccca cgaagtcggt ctgcacttca aaatcttacc
    10921 gtttcgcgga cagttctatc agctgcgtcc cgggtccgca atgcaggtcc gtggcaacat
    10981 ctatccggtc ccggacctgc ggaacccttt cctgggcgtg cactttacgc gacggcctga
    11041 aggcgatgtg acgatcggac cttcggccct cccgctgctg ggtcgtgagc agtatcgagg
    11101 cctgaccggg gccacgctcg gcggtactgt cacgatgctg tcctatttga tgcgactctt
    11161 cggtcgcaac cgggaccatt ttcgctcgat tgcctggacc gagatgatca agctgacgcg
    11221 cacggggttt tttcgtgagg cggaagatct cggacgaggg tttgagaaag ccgatttgct
    11281 gccggggcaa gcgcccggta ttcgggcgca gttggtcgat acgagaacgg cggaactggt
    11341 cagcgatttt gtgattgagc cgggggcgcg ctccacgcat gtgttgaatg ccgtctcgcc
    11401 cgcatttacc tcgtcactgc cgtttgccga gcacgtcgtc gacacgatga ggctcgccta
    11461 gccaggactc ttcatgaatg cctgctcagg cgtccgatcc tcgcgcccgg tgaggccttt
    11521 ctcgttcggc ttcctccatg gactgcgcgt gcacctgcgt cggtatgcgg tctcgaacgc
    11581 ctcgcgacgg cattcatgag gagtccgggt tggcgtttcc ccggctgtat gagcgaattt
    11641 tctggtctga acccgtcgcg agacgttacg attcgattcg tagatgatcg gggcacgtgc
    11701 cttctcctgg gagggccggc ccgctgaatt cgatgatcct gttaccgtgg gggtgatgga
    11761 tgaaaggtgt cgtacttgcc ggagggctcg gctcgcgatt gctgccgctc acgaaggtga
    11821 cgaacaaaca tctcctgcct gtctatgaca agccgatgat ttattatccg atccggacgc
    11881 tggtcaacgc gggcattacg gaagtgatgt tggtgacggg cgggaacagc gccggggatt
    11941 tcctgaagtt gttggggaac gggcgcgatt tcggcctcaa gcatctcagc tatacgtatc
    12001 agcaggggga aggcggtatc gccgatgcgc tacggttggc ggagcatttt gccgacgaag
    12061 gcccgatctg cgtggtgctg ggcgacaatt tgatccaggg caatattgcg aaggcggctg
    12121 aagcgtttcg agtgcagaaa cgcggcgcaa agattcttct gaaggaagta aaagatccac
    12181 aacggttcgg cgtgccgcat ctcgaagggg accgggtgct gagcattgaa gaaaagccgg
    12241 cgcatccccg ctcgtcctat gcggtgaccg ggatctactt ttacgatgcg cgcgtgttcg
    12301 agatcacccg gacgttgaag ccttccggcc gtggtgaact cgaaattacg gatgttaaca
    12361 atgcgtacat tgcctcaggt gaactgacct ggaatgtgct ggacggctgg tggacggatg
    12421 ccggcacgat cgaatcgttg ttggctgcca atcaactcgt cgcgcagacc ggggcgaaca
    12481 ggatggagtt ctaaatgcgt attctcgtga cgggcggggc cggctttatc ggatcgcatc
    12541 tggtgcgtgg cttgattcag agcggccatc attcggtcgt gaacctggat gcgctgaagt
    12601 attccggcaa tcttgaaaat ctcgccgatc tggccggcca tccgcagtac acctttgtgc
    12661 atgccgacat cggtgatcgg caggcggtac atgccgcgtt gcagacgcac cgcattgagg
    12721 gcatcatcaa ttgcgcggcg gaaacgcacg tcgatcggtc gattctcgat cccggggcct
    12781 ttgcccgcac ggatgtcgtg ggtaccggta ttctgttgga ggaagcgcgt caggccgggg
    12841 tgcaacgatt tctccaggtc agcaccgacg aggtctacgg cagtgtcgaa cagggcagtt
    12901 ccacggaggg agatcggctg gaaccgcgga gtccctattc ggcgagcaag gccggaggag
    12961 atctgctggt gctgagttat tggaccactt accggtttcc ggtcgtggtc acgcgcggca
    13021 gcaatactta cggccccaac cagtatccgg aaaaattcat tccgctgttt gcgaccaacg
    13081 cgatcgatgg ggagccgctt ccgttgtacg gtgacggcag gcaatgccgg gactggttgt
    13141 cggtctacga ccatgcggca gggattcagc atgtgttcga acagggggag ccggggaccg
    13201 tctacaatgt cgggggcggc aatgagcggg agaatatcac ggtggccgaa cagatcgtgg
    13261 cgacgctggg gaagtcccgc tcgctgattc gttttgtgca ggaccgcccg gggcacgatc
    13321 gtcgctattc gatcgattgt cgccgcctgc gcgccttggg ttggtcgccg caagtgccct
    13381 tcgaggaggg gctgaagcag accgtcgcgt ggtatcgcac gcatgagagc tggtggcgga
    13441 cgatcaagtc cggagagttc aaagactatt atcaacagca gtatgcgaag cggttgcagc
    13501 aggggacggc gtgcggattg taatcaccgg ggcgcaggga cagttgggca cggatctccg
    13561 gcaggtcttg caggggcatc aactgacgtt gctcgatctc ccgacgttcg acctgacgca
    13621 tcaggactgc ggccgtgtga ttgtcgaagc cgtgcccgag gtcgtgattc acgccggagc
    13681 gcatacggat gtggatgggg cagaacggca tccggccctg gcgctggccg tgaatgccga
    13741 ggggaccgag cgagtggccc gtgcggccgc tctggtcgga gcgaggctca tctatatttc
    13801 cactgattat gtgttcgacg gccggggcac gcgtccctat gtcgaaaccg atccgaccaa
    13861 tcccgtcagc gcctatggcg agtcgaaacg ggcgggtgag cagcgggcct tggcctgttg
    13921 tgagaatacc ctggtggtgc gtaccgcctg gctctatggc ctgcacggcc ggaatttcgt
    13981 caagaccatg ttgcagctgg cttccgaacg accctgtttg aaagttgtag ccgatcagcg
    14041 gggttgcccc actttttccg gagatcttgc cggcatgatc ggaaagctcc tggcgcatcc
    14101 ggcacagggc gtattgcacg tgacaaatgc cgggaactgt acctggcatg aattcgcgac
    14161 ggagattgtc cgcttgtccg ggcgccccgt gccggttgag gcgatcacga cggccgacat
    14221 gccgagaccg gcgaagcgac cggcctattc ggtcttatcg gcagaccgtc tccatcatct
    14281 cgggttcagc atgccatcct ggcaggatgg attgcagcga ttcatgaagg ccctgccggc
    14341 agtgtcctct gcagtttctt agaagtctcg cttcgtattc tcgtttgtat cgggattcat
    14401 ccctctggtc ggacgggtcg tgaagctcgc tcattccgct cgccgcattc ggttcctcgt
    14461 tcccacaggg cgcttagtca gggcgcatcg cgatccacgg tgcattattt tgcgatacct
    14521 cctacgctga gtgtgtcagt aagacacatt tcttgtgcaa cccccgcgat ttctctatac
    14581 tgccgtcctg cagagccctc gtagcgtttc tcgtgacgat gcccggtgaa cgtagccggt
    14641 gagttcgagc ctgtgtgcgc gcctgctccg tcagaccggt aaacgggtga tccacttcac
    14701 gcatgaatac cgacatgccg atccgatccc accgggggcc gagagcgggg tgttcttgtg
    14761 catgaggggc ggttgtgcct tctgcgcgtc gttcgttgcg gtgtatgagc gtccggcctg
    14821 agagggtgaa gtgtatggat gtagttcgtt gtgcctcttt ctgtgacacg cgactctgat
    14881 ggaacgaatg ttgtgacgtc gccgcccgcc gattctgctc tccggtccgt tcacacggac
    14941 gcgcgatccg tttccggcca tgcactcttc tgggcattcc aagggtatgt gatggctgcg
    15001 gtcgcctgtt cgagtttttt cccgatgctg ttccgctatc aggaacatgc cgtcatcgtg
    15061 ctcgtcgtac tttcgctagg gatgtgcgcg atcgagcggg tgagtccctg gatcaaaaat
    15121 ccgctggatc ttcccctgtg gctcttcatg atctgggtgc tctgcaccgt tcccttcgcg
    15181 accgactccg catacagttt tgccgaatgg aaaaaattcg tagcgcaggc cggggtcttt
    15241 tattggtcct tgctcgtgct ggatcgctgt cgacgggaga acttgccgca gcagattctc
    15301 tgggcgctgg cactgaccgg agcggcgctt gcagtctatt cgttggttga ttttgtcgac
    15361 aggggcggca cgtggagaga ccggtatatc cgggccaatg cgttcgggtc ggattataac
    15421 tggctcagta cttacatggt gatgacgatt cccgtggtcg gcagcctcat cgtcctgggt
    15481 cgcctggtct ggtttcgggc ggcacaggcc ttggcgcttg cgttgatggg aggcgcgcag
    15541 cttttttcct atacacgtgg cggatggctt ggtcatgcgg ctcaaggcat cgcatttgcg
    15601 gcgatggtgg gtggccggcg tctggctttg tgggtgctgg gactcttcgc gatggcggga
    15661 gcggggctgc tggtcgcgtc gcaggccgga tttcagcgcg atacggtggc cgccagcact
    15721 gtggacacca ggttgacggt ctgggccatc ggattgcggc aggtggcgac gcaccccgtg
    15781 gtcgggattg gatacgggaa taattcgttc gtgaaaaaat tccctgaata ctcgtcggag
    15841 gcacaggcac aggtgtctga agggactcgc atcattccgg ccatgcacaa cacttttctc
    15901 atggttgcgc tggggagcgg ccttcccgcg ttactcagct ttgtctggat ctttgtcgct
    15961 ctcctgcgcc ggctcattcc gattccctgg cgggctgcat ggaacgatag gtggtcggtg
    16021 ctggctgcgg gtatcgggct ggccgtcatc ggcttcgccg tacgcaatct cttcgactat
    16081 atgtttatgg gcagcctggc gcacatattt tggctgctgg cggcggtcgg catcaccgtg
    16141 acggggtcgg cctggcgagg gggcggatcc ggtcggcagc cggggagccc tcaggatgag
    16201 ggcacctcgc agcagcagcc gtctgcatag gcggcgggtc ggctcacgtc tctagcccgc
    16261 attattcatg agcgcttctg ctgcaatcgc cgatccgctg ctgcgacgag ccttcggccg
    16321 acgggctcgc ccctcggccc ctcgacgtac tgtacgagta cgcctcggga cctcacggct
    16381 ccgcgcgccg gtctcgcgac gtggctcgac gatttcgcga cgaaccgtca tgaataatgc
    16441 gggctagtgt cggtgcggac ctgtactgtc gcttgacacc gcgtcgcaga tctatcacaa
    16501 gtggggtcat tgtggtgtgt cgcctggggg atggatagtg atgtgccggt tccttaggcc
    16561 gggaagaccg atcggagtgc ctgctcaatg aatgcattga tcaccggtat cacgggccag
    16621 gatggttcgt atttggccga atttcttctg gccaagggat acgatgtgta tgggatcatt
    16681 cgccggtcca gttccttcaa cacggcgcgg atcgacggta tttatcagga tccgcatacc
    16741 tcgggcgcca agctgcatct ggtctatgga gatctcaacg atgcgagctc attgaataaa
    16801 atcctccgca ccgtgcaacc cgacgaaatc tacaatctcg gggcgcagag tcatgtcagg
    16861 gtcagcttcg atattcccga atacaccgcg gaaatcacgg gcctgggaac ggttcgattg
    16921 ttggaagcga ttcgtgagtc ggggctgcgt cccaagtttt atcaggcctc atcgagtgaa
    16981 atgtacggca aggtgcagga agtgccgcag cgggaaacca cgccctttta cccccgtagt
    17041 ccctacggcg cggcgaaggt ctatgcccac tggatcacgg tcaattatcg tgaggcttat
    17101 ggcctgttcg cctgtaacgg catccttttc aatcatgaat cgccgcgccg tggcgaaaca
    17161 ttcgtgacgc ggaaaatcac gaaggcggca gcgcggatca aactgggact gcagcaggaa
    17221 ttgtttctcg gcaacttgga cgcgaaacgg gattggggat atgccggaga ttatgtcgag
    17281 gcgatgtggc tcatgctgca gcaggatgag ccggacgact atgtcgtggc gaccggtgaa
    17341 acccacaccg tccgcgagtt tctcgatgtg gcgttcggcc aactggggtt ggactggcag
    17401 cggtatgtga agatcgatcc gcggtattac cgccccaccg aagtcgatct cctcatcggc
    17461 gatccggcga aagcgcagca gaagctcggg tggaaaccga cggtcgattt tcaccgactg
    17521 gcgatcatga tggtggaggc ggatgtggaa gccgaacggc tgaagttgca gggcaccgcg
    17581 tcgaaggggg cctaggtgat cgacacacac gcgcgcattt atgtggccgg gcatcggggg
    17641 atggtgggat cggccatcgt gcgggcgctc gaggcccggg gatgtgacaa tctgctcttc
    17701 cggaccagca aagaattgga tttgcgtgac aataggcgcg ttgccgaatt ttttgcagag
    17761 acgaagcccg actacgtgta tcttgccgcg gcaaaggtgg ggggtattct ggcgaacagc
    17821 acgttccctg ccgaattcat ctatgacaat ctggcgattc agaccaatgt catccatcat
    17881 gcctatgttc acggcgttaa gaaattgctg ctgctgggct catcctgtat ctacccgaga
    17941 gattctcctc agccgatgaa ggaggagtac ctcctgaccg gtccgctgga gccgaccaac
    18001 gagtggtatg ctgtggccaa gatcgcaggc atcaagatgt gccaggcgta tcgccggcaa
    18061 tatggatgcg atttcattgc agccatgccg acgaacctgt atggcccgaa cgacaatttc
    18121 gatcttcaga cggcgcacgt cctggcggca ttgctgcgac gcttccatga ggcgcgtgag
    18181 cagggcaccc ctcccaccct gtggggcacc ggggctccgc ggcgtgagtt tctgcacgtg
    18241 gacgactgcg cggatgcctg cctgttcctg atggattgct attccgatgc gatgatcatg
    18301 aatgtcgggg cagggcagga cattgcgatc gcagaactgg ccgcgctggt cgccgaggtg
    18361 gtgggatatc gaggggacat gcagtgggac cgtgccaagc cggacggcat gccgcggaag
    18421 ttgatggatt ccagccgtat cgccgcactg ggatggaagc cggcgacgtc attggcagac
    18481 ggcttgcgcc ggacctatgg ctggtaccgt cagcagctgg gcgagtctcg atcaggtgtg
    18541 tcatgacggg cggcagcatc agccggtctg tcggtgcgta ggtcggcagg agtgatcatg
    18601 cgggcagccc cgttgagtgc cggtgttggc ggcacggttt cttcctgtcc gttcctcaac
    18661 caagaacccc cctccacagt cgttgatcgt aggaacctct cctcatgtgt ggtgtagtcg
    18721 gagcgctggt cctctcccct ggttcattcc gtatttcaga accctatttg atgcggatga
    18781 gggacagtat ggcccatcgg gggcccgacg gggccgggct gtggatcgac tcgcatgcac
    18841 ggatcggtct ggggcatcgc cggctctcga tcatcgactt gagcgaggcg gcccgtcagc
    18901 cgatggctaa tgaagatgaa accctctggg tggcgttcaa tggggagatc tacaaccata
    18961 tagagatccg gcgcgaattg gaggcgctgg gggggcatcg ctggcggacg gaccattcgg
    19021 ataccgaagt cattctgcat gcgttcgaga cgtggggcat cgactgcgta gacaaattcc
    19081 ggggcatgtt tgcgatcgcg ctctgggatg tgcggcagca ggaattgtgg ttgattcgcg
    19141 accgtatcgg cgtgaagccg ctgtattaca gcctgcacca cggccggctg gtcttcgcct
    19201 ccgagatcaa ggccttgttg caggatccgg agcagccgcg ggcgctgcac gaggaatccc
    19261 tctttcacta tctgtcattt ctcacgacgc cggctccgca aaccctgttt gacggcatca
    19321 agaagctccc cggcgggacc tggcttcgcg tgcgcgccga tggaacgatg gaagaacgtc
    19381 ggtattggga tgtgtgggat cacacgtccc ccctgctcgg ggagacggat gaggccatcg
    19441 ccgctcgagt cctggagaaa ttgcgggagt cggttgcgct tcgcaaggtg agcgatgtgc
    19501 cggtcggcgt gtttctgtcc ggaggtgtcg attctagcac caacgcggcc ctgttctcgc
    19561 agggagacac tcaacgcgtg agaacattta cgatcggtta tgacggtgat tatccgagct
    19621 atcgcaatga actgagtcat gcccggtcca tggcggagca ggtcggtgcg gaccatcacg
    19681 aacggttgct ctccctcgat gatctcatcg agttcttgcc gcgcatggtc gcactgcagg
    19741 atgaaccgat tgccgatccc gtctgtgtcc cggtttttta tgtgtctgaa ctggcccgcc
    19801 ggcatggcgt gatcgtctgc caggtcgggg agggcgccga tgagctgttc tggggctatc
    19861 cctcctggaa aacatccaag atgctcgagg aatggaacca ttggccggtg ccggcggtgc
    19921 ttaaacgtct cggccttcac ggattgcaat ggtgcgggta tggtgagagc attcattacg
    19981 aacggctgcg gcgggggatc cttcatcaac ccgtgttctg gggcggcgcg gaagcctttc
    20041 cggaagcctt gaaacaacgc ctgctctcgc cgcgcctgca gcggcagttt cggcacctga
    20101 cctcatggga agccatccga ccgattcatg accggttccg ggccaaggcc tgggagcagg
    20161 gcccgctgca atggatgagt tatctcgatc tcaatttcag actgcccgaa ttactgctga
    20221 tgcgcgtgga caaaatgagc atgggcgcca gtctggaagc cagggtaccg tttctcgacc
    20281 acgagttcgt gcagttggcg atgagtgttc cagaggcggt gaagacccgt ggcggtgtcg
    20341 tcaaaacgtt gctcaagcag gcagtgcgcg gcgtcattcc ggacgccatc atcgaccggc
    20401 cgaagcaggg atttggcgtc ccggtgcagg aatggatgca gggccgattg ggaacattga
    20461 tgcaggacac ccttgcagat ttttgcgacc ggaccgatat tctcaacaaa gacgcggtgc
    20521 tggatctggt atgtcggcag cgtgatcctc gcagctggta tctctttaac ctggcgctgt
    20581 ggtggaaggc gtatcttgtg tagcgcgttc aatcagagga tcgggtggca tgccagcccc
    20641 tcctgttctc tgtcccgtcg cattcttccg atccagagct tctcccggag cggattccat
    20701 ggctaacgcg gaggatctcg attttgccac tcgtctcctc tcaaaggcgc aacacatcgg
    20761 gtggttagcc acagcccgta tgggcttccg caaggttctt cgtccctggc tctcaaggcg
    20821 gcatcgcgcg cacgtcttgc gccagcctta ccgcgtagat aggctggatc tggccgccgc
    20881 cttcggcgtc actcccgagg acctgaccgc tgccgtagag agagtacgtc tcgcactgcc
    20941 gcagcgtctt ccggtttcac cggagagtgt ggcggagatt cgcgcgctct ataaaaagca
    21001 ggcgccgggt gtgctggagg caaccgtcga atcggccgac cggatatgcg ggcatctgtt
    21061 cgacctcctg ggttccggcc ccgtcgcatt gggagcaacg atcgactggc atcgggattt
    21121 taagtccggc tatcgctgga atcccgatca gtgctttctt gacgtcgccc atggccatgc
    21181 agtcggcgtc gatatcaagg tgccgtggga attgtcacgt ggtcaccacc ttgtgctgct
    21241 ggcccagacc gctctcctga gcggagatga gaagtacgca cgggaatgtt tggcacaaat
    21301 gaccagctgg atcgatgcga atccgaccgc gcatggggtc aactgggcct gtcccatgga
    21361 cgtcgcgttc cgcgcagtca actggttgtg gtcgctgggg ttgttggcag agtcgccgct
    21421 catgacagag gcgtggctga cggaggtact cgcgtcgctg gtggcgcacg gccggttcct
    21481 tatgagcaat ctcgaagtcc gcgacgatgg agtcacgacc aatcactacc tggctgatct
    21541 cgtgggatta ctctatctcg gactctgttt gaaggaagtc cgtgatgcag agggttggaa
    21601 ggcgtttgcg gttcgggaat tggtgcgtga gatggatcgg caggtgctcg ccgacggcgc
    21661 gcactatgag tcgagtctgt cctatcaccg cctggtcacg gaaatgtttc tgtcgtcggc
    21721 catgctgtgc cgtcgccatg gggtggagtt gccgccgtcg ttttatgatc gtttgcggaa
    21781 gatgtgtgag ttcgttcagg gctataccaa gcccaatggt ctggcaccgc agattggaga
    21841 cgccgataac ggtcgcctgc atattctgac cgggtacggg caggccgatg tccgggatca
    21901 ccgccacctg ctcgccgtcg gcgcggtgtt gttcgatcgt cccgactggt gggcggcggc
    21961 cggccttacg tgggtcgaag ggttgtggtt cggcggtctc cgctgtgccg cctgggggag
    22021 ggcgcagggc tcggcgcaga gctcggcgca gagcgggagc cgggcctttc cggaggcagg
    22081 gctctatata ctgcgtcggc aggccgatta tgccttgctc aattgcaatc cgattggatc
    22141 gaggggagtc ggaacccaca agcacaatga cctgctcgcg gtggagatgc acctcggcgg
    22201 ggaagatatt cttgtggatt ccggttgttt tctctatact tccgacccgc aatcctataa
    22261 tcgatttcgc agtaccctct accactcaac cgttagggtg gatcaagcgg agcagaaccg
    22321 tctcattccc gggaagttct tttgtttgca cccggatagc cggctgcatc gtttccaatg
    22381 ggacatcggt ggaccggtgg aacaggccgc ggcggaccac gatggatatg aacgattctc
    22441 ccacccggtg aggcaccggc gcgagttgtg ggccgactgg ctgaacgggt cgtggcgcgt
    22501 cacggatcat gtgttcagtc ccgtgagcgg ttctcatccg catcgtctcg aatggacgtt
    22561 gacctttgcg cctcattgtt cgttgcagcc tgcggaacac ggatggtcgg tggtgacccc
    22621 caggcagcgg ttctggctgg acggtccgcg gttgtccgcg aatggaacgc ctgtggccat
    22681 ctccccgtat ctcgacgagg gaactgtggc ggagcaatat ggcagggcgc ggcgggctcc
    22741 ttttcttcgc tggagttggg agggctcgct tccggtcgaa ggcgtcttca cgattgcctc
    22801 cctggagtca cgttcggcat gagattgcac taggaggccg ggaccgaagt tgaagaaggc
    22861 gttgaccatc tggctgacgg ggttgccctg tgcgggcaag acgaccctgg ccaacatgct
    22921 ctttcgaagc ctgctggagc agggcctgac caacgtggaa gtgttggatg gcgatgtcgt
    22981 ccgcacctat ctttcaaaag ggctcggatt ctcccgtgcc gatcgggaca ccaacatcct
    23041 gcggatcggg tgggtttgcc aggtgttgac caagcacgga gtctgcagca tcgtggctgc
    23101 agtgtcaccc tttcgagccg tccggaacga agtgcgcgcg atgattgaaa aggttggagg
    23161 gccgggatcg tttatcgagg tgtgggtgcg ctgcggggtt gaggaatgta tccggcgcga
    23221 cgtcaaaggc atgtacgcga aggcgttgcg gggagagatt cagcagttca ccggcgtgtc
    23281 cgatccttat gaagagccgc tccaggccga catcgtcgtg gataccagtc gtgaatctcc
    23341 gtctgtatcg gtcgggcgga tcctggaggt ggtcaatcgt ggcaatctct gaccgtggcg
    23401 gtgctcggcg actcagccca aaagggtcgg ccggtggtgg ataggagctt cctgtggttt
    23461 ctgcttccga cgctgctcca aaccctgata ggggtcggcg tcatggttcc gatcacgacg
    23521 tattacctcg acccggccga tcttggcacc gtcgccattc tcactgccct tgccatgctg
    23581 gtgacgccgc ttgcctcgac cggtgacaat tgggtgctct cgacccattg gcacgcgacg
    23641 ccggattccg gccgaaaaga actgctgttc aacctgctgt tggccaatct ggggatgaaa
    23701 accctgtggg tggcggtgtt ctggttgctc gctccggtgg tgttgccgca gctgattcga
    23761 gactatcgcc ccgaatacca gcagtatttc gggttggtgc tgctggggtt gctggccggt
    23821 accacgtggg gaacgctcag tccgttgatg gtcatcgaac gggcgcctgt cagccatgcg
    23881 atgaatgaat cgttgcagtg ggcagccggg gcgaccatga ccttggtggg gctctcggtg
    23941 ttgaagctcg gagtcctggc catgtttctc gcgccgattg ccgcgggcct ggcctccgtg
    24001 ctgcacgggt ttggttatgc ggctcgccga gtaactctgc ggcccagcct gcattggagc
    24061 aaggagatcg ttcgaagcgg cctgccggcg attccattca gtctgatgga tgtgctggcc
    24121 aatagcatgg atcgctttgt cattcagcga tggttggatc tctcgacatt gggcatctat
    24181 gcccattcac aaagttatcg agggatgttc gtcacggtga ccaaggccta ttcgaggacc
    24241 atgacgccca cgttcttgga gttgtttgcc gggaatcctc gccagtcgca gcggcaagtt
    24301 gaagagaccg tgtcgctgtg gtacctctgt gtcacagcag gagggattct ggtcaccctg
    24361 ttctctccgg aggtgatcca cctgttgacg cacgggaagt tcgatcaagc agcggctcta
    24421 gtgccgatct ggtttttgct cgtgtgtgcg cacagcatgg cgataccgtt tacgcagtat
    24481 ctgctcgtgg tgcgtcagaa cgtgctcctg tcctggtcgt ctatcgccat gagtctgggg
    24541 accatagggc tggtttgtct tgccacgtgg cagttcggtg tcatcggagc gacgggcgcg
    24601 gcggtgttag gggccatggc gctacagctg atgcgattcg ttttggcgcg ccggttcggc
    24661 tgtccctacg gcattgaatc cgggatgctg tggggtatgg ggctgctcct ggtgttgtat
    24721 ggggtgacga ctcggatcga gttgtcgctg ccggtgaaag cgttgctggc tcttacagtc
    24781 atcggcgtga ccatcaggca gattgtgcgg cggatttcgg ttcgtgacct gctcaccgcc
    24841 ctcgttccat cgaagtaagt tccgttgtgc aggttacagg caggcttatg aaggtaacgg
    24901 gtcaacatgg gtaaacgggt gctgatcacc ggcggtgccg gtttcgtcgg ctccagtctc
    24961 gcgctgatgt ttcgcgaggc gtatccggat tgggagattg tggccttcga caatctccgg
    25021 cggcgaggat ccgagctgaa cttggatcgc ttacggcgtt gcgggatcag attctgtcat
    25081 ggcgatgtgc ggaaccgctc tgatctggac ggcgtgggga aggtggatac catcatcgaa
    25141 tgttccgccg agccttcggc gctggcaggc ttcgacggcg aggcggccta cgtggtggac
    25201 acgaacctcg ccgggacagt caattgtctg gaactggcca ggcgttatca ggcagagctg
    25261 gtgtttcttt cgacgagccg ggtgtatccc tatgaggcgc tgaacacgct cccgttcctg
    25321 gaaaccccga cgcgattcga ctggcagctg gatcgcgcat gcacgggcgt gtcggcgcaa
    25381 ggagtcgccg aggactttcc gcttgaaggg gtgcgcacgc tgtacggggc gacgaaactg
    25441 tgcgccgagt tgttgattcg tgagtatgtg tcgatgtatg ccgtccgcgc ggtgttgaat
    25501 cgctgcgggg tgctgacggg accctggcag atgggcaagg tcgatcaagg attcgcggtg
    25561 ctctgggcgg cgcggcatca tttcggcggg gccttgtcct atatcggatt cggtggccag
    25621 ggaaaacagg tgcgggacct gctgcatgtg gaggatcttt tcgaattgct ccggctccag
    25681 ctccgtgatc tggcggtcca tagcgggcag gtctacaacg tgggtggggg cagggacgtc
    25741 agcgtgtcgc tgctcgaatt gaccgggtta tgccgggcgg ccaccggaaa gacgatccct
    25801 atccactcgg aggcctcgac gaggccggga gatgtgcgcg tctatctcac cgactcgtca
    25861 aaagtgcggg cggcggcagg gtggaaaccg cggtggaccg tggagcggat cgtggagagc
    25921 attgtcgcct ggatccggga gcgcgagaac gaactcagac cggtcctggg tgagaaccgg
    25981 tgagcgggcg gtttggtcgg ccaacagaat aacggagcgg acatttcgtg cgggttggat
    26041 ttatcgtcca gggattgccg ggagagggat atcacggggg ggccctcacc tgctggtccg
    26101 tcgtgcggga gttgatacgg tccgggcacc aggtctgcgt gatcagtctg ttcgacgtca
    26161 cggagggaaa tccttatctg aagtcccgcg gccaacagga ggccgccttg cgtcgggagg
    26221 gggcggaggt cgtggtgatc gaatatgcca tggaggcctt gaccggcgcg cgcagtcacg
    26281 acccattggc caaattcggc cggtggatcg ggcgtatctg tagcgccccg gacggcttgg
    26341 cctatctgat gccctgggtc aaactggcgg aggaggttcg tcgtgccttg gccggccgtg
    26401 agttcgacgg gtttctgtgt tatcacttcg atgcgctggc ggccgttcat ccgctctgcc
    26461 tctctccgat gctggcggtc gtcggcgacc tctggcactt gcccggttac ttccggtggc
    26521 gcgaaggttc gttttctctg tccaagtatg ccgttcgagg gataagggtc gcgttggaat
    26581 cgatgatcgc cgaacgggcc atgagtacgt tgttggcgtc gaccacctct tgcggcgcgt
    26641 ttgcagggca ctatgccgag tggctgaaag cgcatggctt tccgaagacc ctgtatttca
    26701 gaacgcccgt tcccgatgcg gcgggtccga agtgggacct gttacgaagc gaggcgcgtg
    26761 caggccggac aggagggaag catcgtgtgc tcgtgatcgg agatctcgcg accacctcga
    26821 cttcgtcggg gttgcgggcc gtggcccatg cagtacttcc gcgactggta gacgaattcg
    26881 gtgaggatgg atttgagctg cggctggtcg gcggcggtgc cccgccgaac gacatcgcgc
    26941 ctgccttacg gaagccctat gtgtgctttg ccggacgagt ggtgcccgcc gatccggagt
    27001 ttctcgccgc agacgtcatg atcgtgccga ccaacattcc cctgggcatt cgcgtcagag
    27061 ttatcacggc ctggtcgttc ggctgccccg tggtggctca ccgggcgaac gcgtccggca
    27121 ttcccgaaat gcggcacggt gaaaattcgt tattggcgga tacagatgca caattggtgg
    27181 acgccgtgtt gcgggtcttg accgatcaca ctctgcgaag cgggctctcc gccggtggcc
    27241 ggggaacctt cgaacgatcc ttttctgaat ccgtggcggg acaacagatc gtgaaagaga
    27301 tggagcgact ggccggcgct caggcaacga accggtcatg aaccgtcgat tccggcggta
    27361 gagatcaggc tcatagtttg accgagtgag gggaacaggg tgaagaacgg actgagtgca
    27421 cgaattggca atgggctggc ggcggcgttg ccggaaaaca tctgtgcggc gtttgcaaaa
    27481 cgtctggccg atcgaaatcc gcaactcctg ctggaggcgc tggggcctag actgaaccgg
    27541 acgccgtctc tggataccat gtattttgat ttggctccca ccggcaccct tcaattcgaa
    27601 gacctcgccg ggttgttcgc gagtacctcg ctcgatcatg gggtcattgc catgaccgtc
    27661 cggcagacgg cctacctgtt cggactggtg cgcagaatga aggcgcgcag ggtgatcgag
    27721 atcggccggt acaagggggg ctccacgctc accatcgctg ccgccatgag cggggagggg
    27781 acattctggt cgattgatct cggcgaaaag gaagcgcgtt tgcaccgacg tggcaccagc
    27841 cgcacgttcg acgatgagat tcgcgatatc tgcgcgagat tcggcctgtc cgtcaccttg
    27901 ctggtgggcg attcacggac gattgaagtc gagaccggcg aggtcgatct cgtgttcatc
    27961 gacggcgacc acagttacga gggtgtgaag aatgatttcg aacgcttcgg ccggcgcgtg
    28021 aaagtcggcg gcgcggtcct cttcgacgat gcctgcgatg aagccgtctt tcacacgcat
    28081 tcggaatcgg tcggaaagtt gctggacgaa atcgtagcgc aacaatcgtt ccgcctcgtc
    28141 aagtcggtca accggctggc tcacatcgag cgcgtccgtt aggaggggat ccgtgctggt
    28201 tggtatcttc ggcgccggtc gcaacgggtc gtcgctgctc atgcggctgc tggacggcag
    28261 tcccggcctg tggatctacc cgatcgagct gaactacttg cgcagtttcg cgccgcgttc
    28321 gctgaagagc gccgtgaagc ggacggtagc ctcctgcgcg tcgcatctgt ccggtcaagc
    28381 gggagagacg ttcgagcaac ggcagcggca gttggttcga gcctgggcgg ccgatcagct
    28441 ggatgagctc aagcagacct atctggataa gctggtggag cccatttctc ccgacggcgt
    28501 tccgctcgag cgcatcatgg cgcatatcac cggcgatgcg acgcacgact tggaatccta
    28561 tctggacgcc atccgtctct gttacgaccg gcggacgttg ccgacgccgc cgctgctgat
    28621 gttcaagtcg atcgaagtct cggatctttt ccgctaccag gccatgtttc ccggcatgaa
    28681 atgtcttcac atcatgcggc atccgtattc caattacagc tcgctcaagc gcacggacat
    28741 ggtcttgaag caaaagccgt tctggtttca gggcggcgac attctccgct tacaactgga
    28801 gtctcgttgg attccacatg cggagttcac gctgcagggt cttacgacgg atccgactcg
    28861 gcactatctc gttcgctatg aggatatctg cgattcgccg acccgcacgg tcacggatat
    28921 ctgttcctgg ctgggcgtcg cgcctcccga tgagccgacg gtccaaacgg tcctgggagg
    28981 tcggcatacc aaatcccttc cgatcaattc gagcctgaaa ggcgtggaga cacccgcgga
    29041 ggtcgtgtcc gatatggcga aagcctatgg gtacgatgac atcctgaccg agcgcgagcg
    29101 gaacctgatt ttgctccgga cctatcaact cggtcgccgg ctgggatatt tttccgaaca
    29161 ggatgccgcg caggtcccgg ccaggctgcc gctgttcctg caatggttgg cgccggacca
    29221 atgggagtat atgaacgcct cctcgaaggt ccggttggcg cgcgccctga ttcagcgccg
    29281 gctctatctg tgccgggttc tgctttcgcc gcttgcgtag ccggcgggat ggcgcgatat
    29341 gaaatttgcc gttcgcgata agacgatgtt tcagacggcg ttctcgccta cccgcgagga
    29401 agtgcgcaca ttgctggatt atgtgcggtt tctgtgggag gaggaggtcg gtgcccgcat
    29461 cgcggcgcat ccgctgtatc gaccgtcgga cctctggaag cattgggcgg gacagacatt
    29521 cacagggttc tcgctggaca ccattctgtt gcaaggcggt catgcatttc cgtcctccgt
    29581 cgaagtcggt ttgggcggag agggggggga gaccacatca cgctggctgc gctgtctcgg
    29641 cgcgttattc ggcggcaagg aagccgcccg catccggcaa ggccgggatc cggtgcaatt
    29701 gcagcggcgg ttccgggagt tctatccgca tgtggcggtg tgcccggagc atgaacgctg
    29761 gtatgcgtgg catccgaatc cctatttccg atgcctggaa gcctatgtgt tgcgggagaa
    29821 aagtccgctt ccggaacaga tgctggaagt cggcgcgggg gcctgcgtca acgtggcctt
    29881 ctatcggagc ctgaatccgt ccctgcgcac gacgatcatc gaccttccgg agacgatcat
    29941 gttcggatat tgttttctga aaagtgtgtt tcccgacatc cggattgcat tgccccacga
    30001 ggttggatcg cggcaggccg atgagcgcgc ggatgtccgg tttctgttgc cgacgcaagt
    30061 cgactgggtg ccggacgaca gcatggattt ttgtttcaac atgtcctcgt tccaggaaat
    30121 gaccatcgac acggtgaacc attatctccg tttcatggcg cggacgctca agccgggcgg
    30181 gacactcttg tcggtgaatc tagaaacatc gcgctatctg gaggggaacg cgttgaacca
    30241 cttcgacttt tccctgtacc aggcgacacc tcgggtgcag ccggctccgt tcgggacgga
    30301 cttggtcggg catatccccg ggttgcgcat ggcgcacgcg gaagtggtga attcagcgcg
    30361 gacgccggag gcagtgcggc gctgatgcat gtcctggctg tcattccggc ccgcgggggg
    30421 tcgaaatcga ttcccttgaa aaacatccgt ccgttgaacg gcacaccgct gctggtattt
    30481 acgatccagg ccgccaagcg ctcggcctat cttgaccgct gcgtggtctc gacggatcat
    30541 gtggaaatcg cgagggtcgc gcgagactat ggtgcggagg tcatcgaacg gccggcagat
    30601 ctggcgaccg accaagcccc gaccgaatgg gccctgttgc atgccctgga ggtgttggcc
    30661 aaggacgggt accggccgga gtatgtcgtc accctggagc cgacctctcc gctcaggacc
    30721 acggggctga tcgatcgctg catcgccgag gcgatccggc atccggaact ggattgtgta
    30781 atgacggtgg cggagacccg caagtgttac ggtcgtttgc aggacggccg tttcgaatac
    30841 ctgtttccgg atcagccgag acggcggcag gaacgccagc ccttgtatga ggagagcagc
    30901 acggtgtatg tgaccagaac cgaggtgctg gaacgggacc gatcggtgct gggccgaacg
    30961 caatacgggg tggtcgtgga ggatccgcgc gaagccatcg atatcaatga gcccctggat
    31021 tttattgtgg cggaggcggt gctggcgcag cgtctcgtgg aggaacagca tggctgacat
    31081 cgaatcgatt cccatcggcc cctatcgcat cggaccggac cattcccctc tggtgatcgc
    31141 cgaggcggcg gtcaatcatc agggcgactt cgaaacggcc aaacgcatgg tctatatcgc
    31201 gcatgcgatg ggcgcgcaca tcatcaagtt tcagatccat gtcctggata acgagatgct
    31261 tcgggagacg ccgcaatcgg cgaactttgc cgagccgctc tatgtgacgc tggataaaac
    31321 caacctgtcg atcgatcaac atcgtgagct gaagcgactc tgcgagtcct tgggcatcct
    31381 gtatctctgc acgccgttca gccggatcgg cgcggatatg ctcgaagaac ttggggtggc
    31441 tgcgtacaag accgggtccg gtgaattgac caatctgccg ctgatcgaac atatcggccg
    31501 gaagggcaaa ccgatgatcg tctcgaccgg aatgtgcacg atcgaagagg tcggagagac
    31561 ggtcgcgctg ttgcggcaac tgaagacccc attcgccttg acgcattgtg tctccgcgta
    31621 cccgacgccc tacgaccggg tgaacctggg tatgatcgcc aggtatcgtg agcttttctc
    31681 cgtgccggtc ggcttgtcgg accattcacg aggcatctat accggcatcg gggctgccgc
    31741 tttgggagcc tgcctgatcg aaaagcattt cacgctcgac cggcaacagc cggggcccga
    31801 ccatcccgtg tcgatcgaac cggatgaatt gagtgaactg gtgaaaggcg tggatgccgt
    31861 gttccgcggg cgaggggccg aacggaagat tttcccggag gagcgcgaaa tcgtcgcctg
    31921 ggcgcgcgag agcgtcgtgt cggaagtcgc gatcccgcgc ggcacggtca ttacgccgac
    31981 aatggtttgg gtgaagcggc cgagtccggg gcccggtgcg gtggcggcga aagatttgaa
    32041 gaaagtgatc gggaaggtcg ccaagacaga tattccgaag gacagccaga ttcgctggga
    32101 gcagctgaac gcatgaagaa gcgcaggatt gccgtcgttt ccgaagcccg ggccacctat
    32161 gggtatgtca agggggtgat gcacttgatc gaacagtccg agcggctgga attgcagctc
    32221 atcgtgaccg gcatgcatct cttgaaggag tatggatcgt cgatcaatga aatcgtacgg
    32281 gacgggttta cacctgccgc ccgcgtggac atgtatgtgg gcggggatac accgacggcc
    32341 tggtcgaaat cgctgggcgt cgagatgcag ggtctcgcgc aagtgttcga tatgctgaag
    32401 ccggatcttc tgctggtggc aggggatcgt gccgaaatcc tggccgcgac ggtcacggcg
    32461 gcctatatga atattccggt tgcgcacatc cagtccggcg acctgtccgg ccatatcgac
    32521 gggtctgccc gtcacgccat taccaagctg gctcacatcc accttccggc ttgtgaagat
    32581 tctgccgaac gggtgcggaa aatgggtgaa gagccctggc ggatttttaa cgtcggcgct
    32641 ccgcaactgg acgacgtcgt gcacggcaag aagcttgatc ggaacgagtt ggcgaaggtg
    32701 ttcggggtgg acttcgacaa gccggtcttg ctggtcattc agcatgccgt gttggctgaa
    32761 gtgcatctgg ccagaaagca aatggaagag actctggctg cggtaaagga aagcgggcat
    32821 caggcgctgg tggtgtatcc taacgtggat gcggccggac aggaaatcat cggcgttatc
    32881 cggcagtacg agcaggtccc gacgatcaag acgttcagaa atctggaccg cgaggtgttc
    32941 ctcagtctgt tgactgccgt gtcggtgttg atcggcaact ccagcgtcgg cattctggag
    33001 gcgccttcgt tcaagttgcc ggccctggac atcgggagtc gccagaccgg ccgtatgcgc
    33061 gcctgcaatg tgattacggt gccggaattc gaccgccgtc tgatcggaca ggcgatcgag
    33121 cgcgcgctcc atgacagcca attcagagcg acgctcaaga cctgtgaaaa cccctacggt
    33181 gacggcgaca gttctgaacg gatctgcaaa gtgttggaag atgttgacct cggcaagctc
    33241 ctcaacaaac agatgaccta ttagaggctt ggaattcatg aatgctcctg tattggtcac
    33301 cggcggcgcc ggttgtatcg ggattcaggt ttgccgtgag ctggatcggc gcggtatcga
    33361 ggtgcatttg ctggacttgg gcgagcagat cgcccgtgtc aggaaagctc ttccggagaa
    33421 ggcgaaggtg ttttatggtt cgattctgga tgtctcctcg attcgtgagg ccatggcagg
    33481 ctgtggagca gtcatccatc tggccgcact gctcggtgtg cgacggacgg aggtcaatcg
    33541 cctgcgctgc ctcgaaatca acgtggacgg gacgaagcgg gtgctggact gcgcgatcca
    33601 gcatcgcatc aagcggctgg tgttcgcctc ctcgtcggag gtctacggcg agcccatcga
    33661 gaaccccatc actgaagaaa ccatcacgca ggggaagact gtctacgcgg taagcaaact
    33721 cgccggcgag gaactctgca tcgggtatgc acaacggtat cctgaattcg agcacgtgat
    33781 tctgcgcttc ttcaatgcct acggccccta tcaggccgcc cagttcgtcc tgcccaagtt
    33841 cattcagaat gccatgacga ggaagccgat cgtgatcaac ggcagcggcg atcaaatccg
    33901 ctcctactgt tacagtgagg atacggcccg gggcgtcgtg gaggccctgc tcaggcctga
    33961 agccgtcggt caggtcatca acctcggcaa cagcgaccgg cccacctcat tgaaggagct
    34021 ggcggatctc gtggtcaagg cgagcgggaa tccctcggtt gaaatcaaat atgccgccga
    34081 ctttcaaggc accgaccgtc atgcgagccg tgaaatccat cgccgttact gttccggcga
    34141 gaaggcgaaa cggttattgg ggtttgaatc gcgtgtcaca ctggaagacg gcatccgtcg
    34201 tatcattgaa atgaacagca tttttgaaaa atgggaaagc accgagttgc cgtacctgat
    34261 cgatgaaatg acctaacagg agaccgcacc tcgtccgcgc gccttcatga cccatctcaa
    34321 ctggaaaacg tctctcaagc gcgccctttc tcgcgcgcat gtgctctccg cggtccgcag
    34381 ccttcactat cagttccgcc ggacggtcga tcgtgacttt cgcaaccagg aatcggtacg
    34441 agaacaggag ttccgggaat tttccagtga atacggtgcc gcgttccgcc atctgcttcc
    34501 ggagcagcac gggcttgcgc gacaagtgtt gatcgtgagc tcaggatccc tcgggctgct
    34561 cgttgagatc gcgttggtga aggcgtttca agcggcaggc tatcatccag tggtcttgac
    34621 ggattatgac cgatgggtcg acgcctttta tcgggaagcg ggcatccagg atctgttgtg
    34681 ctgggatgag ttcgtgcggt tgtcgccgga tgcagaagca gttcggatta tggccgatgt
    34741 tgcgtccttc gagcaactcc ttgcattgga ttacggcggc atccgcgtgg ggaaatatgc
    34801 ggcgtccacc gcgttgcgcc atctgcgcgt cggccgcctc gatctcacga accaggcggt
    34861 tcgcgatgcg ctcctgccgt ttcttcaacg atccctatcg tacgcaaaag cggcacaaaa
    34921 cattgtgaag acggtgcgtc cgcagctggt gctgtcggtc gatctcgggt atagcccgag
    34981 aggagaactg ttcgatgtat gcttggcggc cggcctggag accattacgt ggaatgccgc
    35041 ccacaaaaac aaccagctca tgctcaagcg ttacagtcgg gcgaaccggg acgtgcatcc
    35101 ggcctctctt tcggtgcgga catggcacga cctacaagcc cagccctgga ctcacgcaga
    35161 gcgggagcgt ctccgtcgcg aattggtcgg cagttatctg agcggcgaat ggtacggcga
    35221 agtcggcacc cagtttcata cgacgctgca agaggccgcc gagattcggc gagcgttgga
    35281 tcttgacgat cggaaaaaga ccgccgtcat cttctcgcac atcttctggg acgggacgtt
    35341 cttctatgga acggatcttt tcgggagcta tgaggaatgg tttgtcgaga cgatgaaggt
    35401 tgcctgtgcc aatcctcacc tcaattggat cgtcaagatc catcctgcca atatcgtcaa
    35461 gaacgtgcgg gacggagtcg ggtctgagcc gtcggaacta acggcgattc gatcgcttgg
    35521 cgagaccttg ccggcgcatg tccgggtcgt tcaaccggat agccatatga gcacgttgtc
    35581 gctgtattcg gtgatcgact actgtgtgac cgtgcgaggc accgtcggga tcgaggccgc
    35641 ctgtttcggc attcctgtct tgacggccgg cacggggcga tacgatcggc ttggctttac
    35701 catcgactcc gactcgagag cggaatattt gaatcgtctc gcgcacattc aggagattcc
    35761 ctctcagtcg gctgcgcaac gggtgttagc tgaacggttt gctcatggtg tgttcgtctt
    35821 gcggcccttc agtctgcaca cggtgacgtt ggaatatcag cgtgatgcca aggcaagcct
    35881 gcgaaccagg gtctgcctgc cggcgggcgg ggacctgcgg gccgcgacgg atcttcggac
    35941 cctggcggat tggatcaaca gtgggcagga agattttctc gcgccgtctc tttaaggcca
    36001 acaactcaat ggcccccaca atcattccgt gaggacgtcg ttatgtgtgg catagccggg
    36061 atggtgggga caggcgcctc tgaacgggtg cggtccatga ccgacgcgct cgttcatcgc
    36121 gggccggatg acggagggta ctatgacgat cccccgtctc acctgtgtct gggtatgcgg
    36181 cgactcagta ttctcgatct tgagcatgga catcagccga tggcgaaccg ggatcagtcc
    36241 gtctggatcg tttacaacgg cgagatcttc aattcgccgg agttgcgacg acaattggag
    36301 caacgtcagc accggttcgt gacggcgaat tccgataccg aaaccctgct caacctgtac
    36361 gatgagaagc aggagaatat gctggcagat ctgaacggta tgttcgcttt cgtgctgcat
    36421 gaccggaagc gaaacctcct gttcggggcg cgtgatcggc tgggtatcaa gccgttgtac
    36481 tatgccagga cgccggcggc ttttggattt gcttcggagc tgaagagtct gctccggatg
    36541 tccgggttga cacgcgcact cgatgagaac agcttttctc actatctgac gctgcggttc
    36601 gtgcccggga cgtcttccat ggtgaagggg gtttctcgcc ttgcgccggg gcattggttt
    36661 cggtatgagt tggaaaccgg ccggttggag acccaatgtt actggcgtcc ctcctttgct
    36721 ccgcaggaag gtcgttcggc tgcggaatgg agcgagcttg tgcgagcgga gttgcgagcc
    36781 gccgtgcagc gctggagcct cagcgatgtg cccatcggct gttcgttgtc cggtgggttg
    36841 gactcttcca gcatcgtcgg gttgctcgcg gagagcggtc atcgacagct taaaacctac
    36901 tcgcttggct tcgtcggaga aggcgccgcc gcactcgatg aattaccgct ggcgagaacg
    36961 gtggccgagc gctgggggac cgaccatcat gagctggtcc tgcagcccga tgatctgctc
    37021 cgtgatttgc tcaaaatggt gtggtacttg gatgagccgt acggtggagg gttaccgtcc
    37081 tggtatgtct ttcagttcat gcagcgggat gtcaaagtcg gcatgacggg aaccggtggc
    37141 gacgaattat tcggagacta cgggcgctac atcgcattgg agcgtcggac cgtcggccga
    37201 ccggccggcg cgtccacggt cccggcttgg atggaacgtt gctggccgct cgtctcgtcg
    37261 ttgatcgatc gtcttccacg cagagtcgga ggggtgcgct cgagagagcg gtggcgtaac
    37321 tggcccatgg tcggacgcga tccgtttaag tgggactatg tgcatgcgtt ttattacttc
    37381 tccgacgcca tgaagcggac cgacgtgttg gcggacttgg ccgagggaaa gccggaccaa
    37441 gacacggctg agatgttgcg ggactactat cgtcaatcag acagcgcgga gccgaggaac
    37501 gcgttgatgc agttggcgat gcgcactcaa ttgtcggaag agtttctctt catgaccgat
    37561 cgattttcga tggcacattc gctcgaagcg cgggtgccgt ttttggacca tacgttcgtt
    37621 gaggcggtga tgcggattcc gtcacacatt cgcaccaagc cgggggatcc gaaatatttg
    37681 ctgcgacagg cgatgaaaga cttgctgccg agcgagctgc ttcacgcgcc caaacggggg
    37741 ttcgtgattc cgacggcaca atggctccgc ggcccgctgc gtcctttggc tgaacggtta
    37801 ttggcgccga aacggctggc tgatcaagga ctcttccgac ctgagttcta tgcacggttt
    37861 gtccagcctc atctggaagg gcaggcagat tatcatcctc aagtctggac cgctctgatg
    37921 tttcaggtct ggcatagtct gtatctcgaa ctcgccctta cggattgccc gaatttttcc
    37981 tggcaggagt tgtgttaagc catgctggtg agaccctctc gtgactatta aatctccggt
    38041 atcgatctga atgggtgtgc ggtagaaggg agcgtggaga agtacgatgc gtcgggagca
    38101 tatctctgag ttagtctgcg ccggtggaca acaccgactg aatttgcagg aaggtgcatt
    38161 tgaggaacag ggggatatca aagagggagc gttgacttgc gtggccacct gcccgacggt
    38221 accgatccgt gggtctgtgc cgcggtttgt gccggacagc ggctactcac aaaatttcgg
    38281 ggagcagtgg aatcattttc gccggacaca aatcgataag ttcaatggga cggatttgtc
    38341 gaaggagcga ttctacagcg ggaccggatg gtcacctgcc gaactcaaag gcgccaagat
    38401 tctggaggcc ggttgtggtg cgggacgctt cactcaagtc atgttggatg ccggtgctca
    38461 ggtatattcc gtcgacctca gctccgccgt agatgcctgt tttgccaata acggccctcg
    38521 tgaaaacctg tgcgtcgttc aggccgatct ctgtcggatt ccctttcggc atcattcgtt
    38581 cgacaaggtc ttctgttatg gagtgctgca gcacacgcct gatccgcacg cgtccttcat
    38641 gagtctcatc ccttttctca aaccgggtgg cgacctggcc gtggatgtgt atctgaaggg
    38701 gtgggcgttg gagccctaca agagtaaata tctttatcgg ccgctcacca cgagaatgcc
    38761 gcgtcacctg ctgtttcgat ttctccagtg gtacatcccc aaatggcttc ccgtcgatac
    38821 gttcatcaaa cgccttcctc ttgtaggccg ggtgctgggc atgctgattc cttgctggaa
    38881 ctatcattat ctgcccttgt ctcagcaaca aaaaaccgaa tggggaatcc tcgacacgtt
    38941 tgacgccttg gcccccgcct atgattaccc gcagactccc gagacggtca ccgaatggtt
    39001 cacaagtgct ggattaatgg atatccgggt acggctgggc ggcaatggcg tgctcgggaa
    39061 cgggagaacg cggcccttcc cggtgtaacg gaccgacatg aatattctgc acctcagcac
    39121 ggaatacaat ctcggcgggt ccggccgggc cgcctatcga atccattcga gcctcctgcg
    39181 acgaggccat acctcgcgta tgcttgtttc gggactggtg cacggcgtgc cggaggcggg
    39241 accaatttgg ggcaacatgc catggcgggc agcggattgg ctggcgcgcc gggcgaccga
    39301 tgccttgagt ctgcagtatc tgtttttgcc ctcgtcctgg cgattgctgc ggcatccctg
    39361 gttccaggct gccgatgtcg tgcagctcta caatacgcat ggcggctatt tcagccattc
    39421 ggtcttgggc gtggccagtc ggcgcaagcc gttcgtctgg cgtttgtcgg acatgtggcc
    39481 cttgaccggc cattgctgtt attcctataa ctgcgaccgg tggaaaacag gatgcggggc
    39541 ctgcccgctg ttgcacgacg agcctgcctt gaaggcggac cggacggcgc tgctgtggag
    39601 aacgaagcag cgcatttatg ccggagcgaa cgtcacactg gtggcgccgt cgcggtggat
    39661 cgccggactt accgaacaaa gtccgctggt cgggcattgg cctgtgcagg tgattccgaa
    39721 cggtctggac ttgagggtgt ttcgaccgat cgagcaagcg gcagcccggg aagtgttggg
    39781 gctcccacaa ggtgaagatc tggtgctgtt cagttccgtg gagactcaag cctatcgcaa
    39841 gggcggcaca ttcgtcgcgg aagccgtgaa cgccatcaag gggaagactc ggaagccctt
    39901 tcgcctgttg gtggtgggca accgcgctag tgagtgggag accgcagtca gtgtccctgt
    39961 gacagccatg gagacggtga aagacgatta tctgttagcc gtcctctatt cggctgcgga
    40021 tctgttcgtg cacccggccc tggcggataa cctgccgaac ggcgtgctgg aaagtctggc
    40081 ctgtgggact ccggtggtgg cgttcgacgt gggcggtgtg ggagaggcgg tgcggccgat
    40141 ggagaccggt tacttggcgc gcgacaagga taccggcgac ctgtcggagg gcatcaagcg
    40201 tctcctggac gatcctgatc tgcggcgacg actgcgcaca cggtgtcgtg aggttgcgga
    40261 aaccgagtac gatatggcgt tacaaacccg acggttcgag gagttgtatg agacggtgat
    40321 ccatggccga tagccgggcc gagaatttct ccgaaacttc ttacggcatc gtgaagcggt
    40381 tgcagaccat cgaggcctgg acggaccggg tggcgcaacg gctgggaaaa ccatcgctgt
    40441 ccatcctcga cttcggctgc ggcacaggcg atcatgtgac ctatccattg gcctgtctgg
    40501 gtcatcgcgt gttgggggtg gatgttcatg ccgcctctat ccaagaggcc tcgggaaagt
    40561 atcgcctgcc gacccttcga ttccgcaggg cggagatcga cgatctactc aaggaggatg
    40621 tgcggttcga cctgatcgtc tgttccgagg tgctcgaaca tctgcgcgag ccgcagccgt
    40681 ttcttgaagc gctcggccgg ttgttgcatg accggggcgc gctgatcctc acgaccccga
    40741 acggatacgg ggcgttcgaa tggctgacga gtcttcagaa ggcgctcagt cgggtaggat
    40801 tgcatgagtt ctttcgacag gtcgcccgtc tcttgctggg gaaacaggcc ctgtcccccg
    40861 ccgggagagg ggcggatgcg gggcacgtgc cggtctgttc tgccgggtat ttgaacatgg
    40921 actccacgca tgtgcagttc ttccgaatcg ccgggctcga agccattctt gcgcagagcg
    40981 gattccgcat cgtcgaacgc cgggcgcgca catcgttttg tggaccctat gtggatgtgc
    41041 tgtttcgcgt gctccccttc gagcaggcgc tctgtcgcat gaacaatcga ctggcggatc
    41101 tcctcccctt cagatgggcc gccgattgga tgtttctggt tgagcctcaa ggcaagagcc
    41161 ctgcatgagt gtcgaattgc cgagggtatt ggtcgtgacc gcgaacaatt tcaatctggt
    41221 cagcggcggc gggattacct taacgaatct ctttcgcggt tggccgcatg accggttggc
    41281 aaacgtgcat gaagatgcgg tgccggaaga tcacagcgtc tgccggacct tctatcgcct
    41341 gaccgaggag gagattcgct ggaactggcc gttttcactg ttacaaggct ggtacggccg
    41401 gaaaaaacag caggcgattt cgacaggggg ttccgcgcca ggaccagccg ggcaagggtg
    41461 gctgagcgcc gtgaaacgcc ttctgggaga cggcgctcca aaacaggcga ggctcactcc
    41521 ggcactcctc gactgggtgg agaaatttca cccgacccta ctgtacgggt ttctgggttc
    41581 attggagcaa atcaccttga ttcgatcgct ggcggcccgc ttcaacattc cgatggtggt
    41641 gcacatgatg gacgattggc ctgcggtgtt gcaccgccgc ggattgctca gtccgatcat
    41701 gggccccctc atccgctccg aacttgcctg tgccttgaag gaggcgcggg gacggctggc
    41761 gatctgcgac gacatgtgca cggaatatcg cgatcgatac gggtatgagt tcaccgcctt
    41821 tcagaatgcg ctcgatccag ccggatggtt gccccatgcg cggcatcagt ggcaggccgg
    41881 agtgccgttc gttgtccggt atgtgggttc gattttggct gatggtcagc aggaaagctt
    41941 gaaggatatc gccttggcgg tgacaggctt gcggcgcgcg ggacggccga tacaattatg
    42001 gatccatgcg cccggacctg acgccgacta tcttcgccgt ctcgggatgc ctgcggatgc
    42061 ggtggtgatc gaaggaccgc ctgatccggt gctcgtgccg cgattgctgg ccgagtcgga
    42121 tctcttgctg ttgccgtaca atttcgatgt gcgtagcgcc aggtatattc gactctcgct
    42181 tccgaccaag gcgccggcgt acatgatatc cgcgacaccg gtgttagtgt atgctccgtc
    42241 cgatgtcgca accgtccgtt atgccgcgcg tgaaggctgg gggtatgtag tgtcttcgca
    42301 gggtgaacag gcggtggcgg aggcgttgac gcttctcatg gacgatcaga cgttacggga
    42361 acggctcggg aggaaggcgc aacaggtggc tttgctccgg catgatgccg gccgggtgcg
    42421 gcaggccttc tgggaaaccc tcacggcggc ggctcgagtg tcgtagccgg gagccccgtc
    42481 gcggggcctg ttcccgacgt aggcaatggt ttgcacggat gaggcctggt gtggagaaga
    42541 gagagacggg atgcgaaaag ggcggcaggt gccggtgatg acagagtatg gggccgattc
    42601 acagactgtg tcgtcgcaga acgaggatgt tcgacagtat tgggatcagg aagcctgcgg
    42661 gacgtataag tctgtggtgg gcgctctgcc ggaattgagc cgcgaatggt tcgcgtcggt
    42721 ggagcagcat cggtatgcca tggagccatt catccatacg atcgcggagt tccccacgca
    42781 tcgcggaaaa accgtgctgg aggtcggggt gggggccggg acggaccacc ttcaatgggc
    42841 ccgcgccggg gcggtctgtt tcggtgtgga tcttacgcaa cgcgcgatcg acacgacgcg
    42901 cgctcatctg acgctgcatg gattcagttc gaccctgcag cggatcgatg ccgaaacatt
    42961 gccgtttcca gatgcgtcat tcgatgtcgt gtattcctgg ggtgtgatac accactcggc
    43021 ccgtcctgaa cagatcgtcc gggaaatcag gcgggtcctc aagccggagg gcatgttcat
    43081 cggcatgctg tatgcccggc attccctcgt agtattcaaa ctgtgggtga agcatgcctt
    43141 actcaaggga cggccttggc gatcctgggc cgatctggtc gcgcatcaca tggaaagccc
    43201 gggaaccaag gcttataccg ttccggaact gaacgcattg tggtccgagt tttcgtcttg
    43261 cgagaccacc cagattctga ctccctacga ctacaattgg tttccgggtt ggatgcgacg
    43321 atggttcagg gatcgatggg gtttctttat tgccgttcgt gctcggaaat agaatggcgc
    43381 gagtgagacg aagggattcg cgcgggaact ggtatgaggc ctaaacggtt gctcatggcc
    43441 agcgccaact attggcgctc tcccttccag gttgggagcc accatctggc ccgcggcttc
    43501 gtgcgtgccg gctgggaggt ggcgttcgtg tccgatccga tttcgccatg gcatctcttc
    43561 ggcgggcagc tcaaggatct gcgggaacgg tacgaactct accgcggtaa cggggtcacg
    43621 gattgcgatg gtcatctctg ggcctatgtt cccggcgccc tgctcacccc ccacaataaa
    43681 cctctgctgc ggagccgatg gaccggggag cgatgggcct cgttgagtct gccctcgctg
    43741 atggacaggc tctgggtaag aggatttgac gaagtcgatc tcctgtattg cgatagcgtg
    43801 gcgcatgtcg gctggatcaa ggacatcgtc aggcgcaagt cggtctaccg cgtcaccgat
    43861 tgcctggctg gatttcagaa aaccacacct gttgcgttgg agctggagaa ggagctggcc
    43921 gggtcggtgg atctggtggt gtatgccgcc cggagtttag aggactatgt gaagcaactg
    43981 atgccgaagt ccatggccta tctgccgaat gcggtgaact atgcgcactt cggccaacag
    44041 tcttgcgcac gcccgccgga atatgatgcg attccgggtc cgatcgcgtt gtatgtcggg
    44101 gcgatggatg tctggttcga ttaccggttg ctggatgagg cggcggcccg gttgccggag
    44161 atctcattcg tcttgatcgg acctgatgcg ctggccaagc aacggctgcg cgcgcggccg
    44221 aatctgcatc tgctcggcaa acgtgaatac cgcgagttgc cccgttatct gcagcatgcg
    44281 gatgtcgggc tgattccatt cgatgtggcc aatcattcgg agttggtgag aagcattcac
    44341 ccgctgaagt tgtatgagta ttttgcctgc gggctgccgg tggtcgccgt ggagtgggag
    44401 gagttgacct atctccggag ccccgcccat ttatgccgtg gaaccggaga gttcatcgcc
    44461 gcgatcgaac aggccattgc gaatccggcg gagacatcca cgttgcagag ctatgccgcg
    44521 gggcacgatt ggagtgagcg ggtgaaggcc attctttccc atctcggata ggcgttggac
    44581 gcacatgatc gtcgacgttt gcctccggcc cggccgtgaa tcctgctggg cgggtctcac
    44641 acgtcggccg ttgatgaacg acggcagcca gccgccatca ggaagcagca agtgccgatg
    44701 tttcattccg caggtcgtgc cggttatcga tgacttccac tgaaacacat ctgtcgggtc
    44761 ctgatcgggt cggattgggt ctgctcgcgc tgttcctctt ctcgccggtg atgttcaaaa
    44821 ttcaagtgag tcagggggtg gccatccatc cctgcgcgcc gttgctggct gccgcatggg
    44881 ggtggatcgc ctggatgatc gggcgcacgc aggtgacgag gtctgagaag atgtggctga
    44941 tcgagcggca ggcctggaat atcccgatga tcctgtcgtt tctggtgatc ggcgggttgg
    45001 cgctcagtct ggcgatcaac gggttgcggc tcggatcgtt ctcgtccgca ggatggttgc
    45061 tcctgttcaa gtgggccttg tacctggcgc cgcttcccct tgcggccttg ctcgtgatga
    45121 ggacggggcg ccgtgtgttg caattggcgg cgtggctgat tcctgccgtg gcgtttctca
    45181 cgctcctcta tacggccgcg cgttttctcc aggctgccgg gggggagtac ttcaatgcct
    45241 acttcgacgg caaggcgatg tttctggcga tgggaatgat gggagaagtt ctttctgccg
    45301 aggggcttac cgtccggtcc gatacggtga gccatggcgc ctacggcatg tatttggtcc
    45361 tggtgctggc attcagtagc agcctcgcct ggttgcgggg atggaacggc atccttccgg
    45421 cctcgtatgc ggtggcgcag ggcatcattc ttggtccgtt cttggtgctc ggggtgctat
    45481 tgaccgggtc tcgatccagc cttgttctgc tggccggtat gctgctgatg ttcttaacgt
    45541 tgctgatcgc caatcccggt cacgcgcagc cgccacagcg acgtatcgcc gccgcggcgt
    45601 tggtgctcac cgtatcggcg tgcctattct tccttccccg ctggttcggc ccgatcttcc
    45661 cgacgcttga gcgcatgcat caaaccgtcg atcaaccgct tgagcttcag gcgacgctgt
    45721 tgggccggca gaccccggat ttcgaccccg gcacgcaagc gaacgtcgcc gtgcgaaatg
    45781 cgcagagccg ggtgtggttg tgggggcaaa cctttcgcta tatgaccgag cacccgctga
    45841 cgctcatcac cggtatcgga tacgatcgtc gacgattcgt tgaacaggtc gtcggcttgc
    45901 cgtacgtcgg cacgaatatc gaattgcgaa cggcgcacaa tctgtacctc gatgtggcca
    45961 tcaagggggg aatcgcgccg tttgtgagtc tggttctcct ctgtctatgg ttgctgtgga
    46021 cggcctggac aagcgtgtgg atcacgacgt cggcagggcc aggcgcggag ttgtacggag
    46081 cgggatgggt gatcctgaca ctctggcccc cgctgctgct ggtcagcggc ctgggcgaag
    46141 atctggtgac cgacaatctg ctgttgcatt ggacgacgct gttcggattg gtgttgggcc
    46201 tgcgcggggc catcctgcga tctgcgatgg ccgggcggat cgtgcatctg tcagccacgg
    46261 cggggctcgg cgggggaccg gcctatatca cggcagtggc gcgtcaccag gtacaccatg
    46321 caacagaggt gcgcgtggtg tgttcggacg aaaaacccta tgtggatatc tggagagaaa
    46381 tgggcgtgcg ggtgaccgta ttgccgatgc gtcgcccgaa cgctcggacg atatggacta
    46441 tcgtggggct catgctgcgc gatccggccc ctgttcatgc tcatgggcgc ggcgccgcgt
    46501 ttttcgcgtt ctggatcaag atgtgcgtca gggttccggt ggtctacacc ccccacggcc
    46561 cgcactatgc ctatatgcga ggatggcgat tcgtaccggc ctggtgcgga gaatacctgt
    46621 ttcgaatcgt cttcgatgcg gtcctctatg tgtcctccgg cgagcgggat cttgccgagt
    46681 cgttgcggct tcccatgcga agctcgcggg tggtgctctc cggtatgatt tctgatccgg
    46741 gccacgaatt ggaacaacct gcccggcgtg aggcggtgcg ccgggaggtc ggcgtcgcac
    46801 catcccaatt cgtgatcgga tggatcggtc gctttcatca tcagaagggg ctggacattt
    46861 tccttgcctc gattcctgag gttgcggctc gtgtgccgga cgctgtctgg ctcgtcatcg
    46921 gggaagggga accgggagcg gtcgaacggc atcgtgagac gatgcgacgg ctgggactcg
    46981 gcgaaaaggt aacgttcttg ggcggccgac ccgacgccga tcaggtggct ggtgtctgtg
    47041 atctgtatat ctccacctcg cgttgggagg gactccccct ggtccttctc gaggtgatgg
    47101 ctcgcgatgt gccgatcgtt gcgagcgacg tggtcggtaa tggcgatgtc cttgacgggt
    47161 ggggtgtctt gttccccccg aatgatgcgg cggcggccgc ggaagcccag gtggcgttgg
    47221 cgacggaccc gatgaagcgg caggccttgg cgcgtcgcgg ccgtgcagtg ctgcacgagc
    47281 ggtttctgct gacgcgaatg cttcgggatc tggatcacgt ctatcgggat gtgtggggtt
    47341 cggaggtgtt gtcgtgaggg ccgtcgccga ttggtattca acagcaggat ttctggccaa
    47401 gctgggtaag acggctctcc cgttagtggc ctcggatgcc gtgctgcagg ggtgccggta
    47461 tggcttggtg gcctatctgg gaatcctgtc gctttctctc ctgggctcgt atctgttcgg
    47521 ctctgcgatc ggggccctgg ctgccgtggc cattgatttc ggcatcaatc aacattggct
    47581 ccggctggac tccgtcgcgg gggggcttac gcggcaggcc tttacgcggg ttctcgcggt
    47641 gaaactggtg ttgtcgctga tcctcgtcgc cggattcgtc ctgttgatgg agggcggctt
    47701 ctggagcaga ccatttccag ccatggtcgt aaccggtctg atcatggcca acctccatgc
    47761 gctggtggag acctgcgagt ccgtcgggtt cctgttccga cgtcatcatc tggtcgccct
    47821 ggtgcgggtg gtgctcgggc tcatgatgta tgccatgccg gctgcagcgg ctctattcat
    47881 gggccgcccg tccggagagg tcatgattca acccctgctg tcggcaggtc tgctcgccgg
    47941 cctgctgatt ttgtcagggt acgtctggaa gacctcccgg atgctggtgg acgggccaga
    48001 atcggaacag gggtacctcc gcacctggcg ggcgtcccgg tggttgggcc tcaatcagct
    48061 cgcgatcgtg gtggatgtcc gggctcctct ggtgctcttg ggtttgttgc tcggggaaac
    48121 ggccgtcggc ctctacgggt tcgtgcaacg cacgaccgcg gtggtcgagt tggcctgggc
    48181 gtcgatctcc aaattgctgc tcaagtctta tgcggaggac gcctccctca agggaggacg
    48241 cgtggtgcag caacatgtgc ggcgcgccag ttgggtgacc gggacggcca tgatcgtcgg
    48301 tattgccggc gtctggatcg gagtccacta catcgagcga ctggaccgat ggtcggagga
    48361 cgtgcgaacc ggactggccc tgttgcggtg ggctgcagtg gccatcggcc tcagttccct
    48421 caagcgacca ctggtgctgg gcttgattgc cttgcatcat gaacgagagg tctgcaccgt
    48481 caacgtcctc tcggccgcgg ccgggttaac cttcattccg tttctgattt ggtcgctgag
    48541 tatctggggg cctctcgtag cggcggtggg atgcgaaacg ggggccatcg tcctgctcgc
    48601 catctatttt cgcgccgcgc tgcgcagact tgaagctgag accgatcacg tccgaaacag
    48661 tcggtgggtg tcgggagaac ccgcccgcta cgatcgatcc atatgaaggt ggcgatcgtc
    48721 catgattggc tcaccgggat gcggggcggc gagcgctgtc tggaggcgtt ctgcgagctg
    48781 ttccccgatg ccgatctcta caccctgctg cacatcaagg ggacggtgtc gcccacgatc
    48841 gagcgtcacc ggattacaac tagtttcgtg caatccttcc cattggccgc gcgatactat
    48901 cggtattatt tgcccgtctt tccttttgcg atcgagcgct ttcggctgcc gtcgtacgat
    48961 ctggttttaa gctcgagcca ttgtgtggcg aagggcatcc gcccgccggc gggagggaaa
    49021 catctttgct acatccattc gcccatgcgg tacgtgtggg atcagttcga caactatgcc
    49081 cgcggcgggc gatcgggcgt cgtggcgaga ttgggcatgg agctgttccg aaagcgtttg
    49141 caggcctggg atgtggcctc cgccgcgcga gtggatcagt ttgtggccaa ttcccacaat
    49201 atcgccggga aggtgcaacg gtattacaac cggccggcgg ctgtgctgca tccaccggtc
    49261 gattgggagt catttcaagc cgcggaacag tcagaagact tctatttgat ggtcacggcc
    49321 tttgctccgt acaaacgggt tgatctggct atccaggcgt gtaatgttat gaaacgacgc
    49381 ctgaagatca tcggcaaggg gcaagaggag gcgcggttgc gcaagctcgc cggcccgacg
    49441 gttgaatttc tgggctggca gccggacgat gtggtgaggg atcactatgc ccgctgtcgg
    49501 gcgttgctct ttccaggcga ggaagatttt ggtatcgtcc cattggaagc gatggcctgc
    49561 ggtaagcctg tgcttgcctt cggccgcgga ggagcgttgg agaccgtggt gccgttgccg
    49621 ggtgccggat ccgccgaggg cggacctggg caagggggct ccggttctgt gaccagtgcg
    49681 gggaaggttg ccacgggagt gtttttcttc actcaggcgg tggaatccgt tgtcgacgcg
    49741 atggagtgtt tcgagcgtcg ccggacagaa ttcgatccgc atgccattcg tgaccacgtg
    49801 gcggcattcg accggcggct tttcaaggag cgcgtgaaag ccttcgcgat gggggcgctg
    49861 accggtacgg cttcatagag ggagcatgct caaacgccac tcccaatttc tcaagagtct
    49921 gctgttctgt atcgatctcg ggctgatctg cgcctgctgg gtgggagcct actaccttcg
    49981 tttttcagag atcgtggagc cggccaccaa gggcattcct ccgctccgga tctatctgct
    50041 gttgctggtg ccgatcatcg cagtctgggg catgtcgttc caggccttcg atctctaccg
    50101 cccgcgacgg atgggcacgc ggctgtcgga gtttctcgac gtggcgaaag ccaataccct
    50161 gtccgtgctg atcctggtcg cgttgacgtt tttcttccgg cagtacgaat attcccgact
    50221 ggtgttgctc tatttctggc tgttgaacct catcaccctg ggcttttcgc gggtgctgtt
    50281 tcgggaggcg cttcggttcc tgcgtcggca cggatacaat caacgccatg cgctggtcat
    50341 cggcaccagc cggctgggac gccgggtggt ggatgcgctc gctcagcatc cggaactggg
    50401 ggtgaaagtg catgggttct tgagcgccga ttcgcggaag gtgggggagc ggatcaacgg
    50461 ggtgccggtg atcgggcggt atgacgacct ggaggaacgt gttcagtcag gtgtcgatat
    50521 cgtctttgtc tgcctgcctc cggaggacga gcagtgggct gaaaaaatgt ttgcttgttt
    50581 ggccacgacg atggtcgaag tgaaggcctt gcctgcgatg tgcgaatttg tgagcctgcg
    50641 cgcggaggcc gagatgttcg agggccttcc gctcatcacg ttgcagggat cgccgttgta
    50701 cggttggaat ctggtcatca aacgggtgtt ggatgtggtc ggcgcatcgg ccgcattggt
    50761 tgtattctcc ccggtgttat gcgcaatcgc ggtcttggtg aaactgactt cgcccggtcc
    50821 ggtctttttc cgtcaactcc gcatgggatt ggacgggcag gcgttcgaaa tgctgaaatt
    50881 ccgttccatg aagctcaatg cggagtcgga aaccggcccg gtctggacgc aacccaatga
    50941 cgatcgccgg acgccgatcg gcgcgttttt acgccgaacc agcttggatg agttgccgca
    51001 attctggaac gtgctgcgag gggaaatgag catcgtcggt cccaggcccg aacggcccga
    51061 gttcatcgcc cggtttcggg aaacgctgcc gcagtacatg ttgcggcaca agatgaaagc
    51121 cgggatcacc gggtgggcgc agatcaacgg ctggcggggt aacacgtcgc tggaacggcg
    51181 gatcgagcac gacctctact acattgaaca ctggtcgatc tggttcgaca tcaagatcat
    51241 gctcctcaca gcttggcgag ggtttatcca ccggcatgcc tattgatgtt tcgtgccgcc
    51301 gtcccgaacc ggctgcttcc gttcggacct gtcttgtagc gcgtcggata ctcgacatgc
    51361 ccgagttttc ttgagtttca ggcgatggct tgctaacatc gccctcagct gaaagccatt
    51421 gcatgcctag gacaacacgc cgcacaaaat ctccagcgaa gcctcgtttc tccttacaag
    51481 ggactctccg ggggatccgg ttattcgcga cggatgtgga tggagtcttg accgatgcgg
    51541 gcatgtacta ttccgagtcc ggcgacgaat ggaaaaaatt caacacgcgt gacgggatgg
    51601 gaatcaaact cctgcagaag gcgggcattg tgacggcgat catcacgcaa gagtcgacca
    51661 agatcgtgat gcgccgcgcg caaaagctga cgattcccga agtgcaccag ggcgtgtatg
    51721 acaagctcgc cgtgctcaag gagctgattg cacggcatgg cttctcgatg gatcaggtgg
    51781 cctacatcgg tgatgacgtg aacgatctcc aggccctggg cgcagtcggg ttttcagccg
    51841 ccccgtccga cggaatgcct ccggtgctga agacggttcg atatgtgtgc aagaaaaagg
    51901 gcggagaggg cgccgtgcgg gaagtggccg atctcattct ggcggctcgg cagccacgct
    51961 gagctattca ccatcacgct atgtcgaaga tgaagggcgc tccatggata tgacggaaca
    52021 tctctatccg gttattctag ccggcggcag cggaacgcgg ttctggccgc tcagccgtca
    52081 tctctatccg aagcaattgc tacggatcat cggcgatgaa acgcttattc agcagacgat
    52141 gcggcgcgtc ctctcctgtg cgaagcccga gcaggtcatc atctcgacca atccgggcca
    52201 ggcggattcg attcgggtgc aattgggaga gtggaagtcc gcgttgaaaa ataactacgt
    52261 ggtcgagccg gtcggtcgca atacggcccc cgccattgcg ctggtggcgg ccgagcttct
    52321 gctgcgcgat cctcatgcga tgatgctggt cttgcctgcg gatcatgtcg tgaccggtga
    52381 gaaggcgttc aaggcggcgg tggcgctggg tgtccagctg gccgagcaag gacatctggt
    52441 gacgttcggg atcaagccga ccagaccgga aacggggtat gggtatattc agcccaaccg
    52501 gcggaagtct ctggccaaga agggacggct gacgggccat ccggtcgcgc gatttgtcga
    52561 gaagccgaac cggaccaagg cggcacagta cttgaagaac ggtcacttct attggaacag
    52621 cgggatgttt ctctggaagg ccgccactat tctcgaagaa attcagcagc accagccgca
    52681 actggccaag gcgattcagc gggtccatgc tctcatggcc tcgggtgctg agccggggct
    52741 ggtcgaggca gcctataaga aagtgccctc ggtgtcgatc gataacgggg tcatggaaca
    52801 gtcggccaat gcagcgatga ttcccgtcgg attcggatgg tcggacgtgg gcaattggag
    52861 cagcctcgaa gaggtcgcgc cacgcgacaa ggccgggaac gtcgtcagcg ggcgcgtcat
    52921 cgatatggac agcaccaatt ccgtgctgta cgccgatcgc cgggtcgtcg cgaccatcgg
    52981 gctttccgac atggtggtgg tcgatacgcc ggatgcgacg ttgatctgtc cgaagtcgcg
    53041 ctcacaagat gtgaagcaga tggttgagat ccttaaacag cagggagctc ctgaacactt
    53101 ggagcacttg accgtgtttc gtccgtgggg atcctacacg gtcttggaag aaggacctgg
    53161 atataaggtc aagcgggtga cggtgaatcc cggagggcgc ctgtcgctgc aactccatca
    53221 taagcgcagt gagcactggg tcgtcatcgc cgggactgcg cgggtcacga ggggagacga
    53281 gctcctggat ctgcgggtag ggcagagcac agccatcccg gtcgagacgc ctcatcgcct
    53341 tgaaaatctc ggctcggaga cgttgcatat catcgaagtt cagaacggcc cctacctcgg
    53401 ggaggatgac atcgtgcgtt ttcaggacgc ctacggccga acctccgtac ggtgacgggt
    53461 gactggaaaa ttggtgacgt acgtgccgtg aagaatcgtc agatcaccac atcgaacctc
    53521 ggactcggag taatgcatgg cactgtttcg tgaatatgat ttgaggggcg ttgtcggaga
    53581 agaactcacg gaagccatcg ccgagcaggt ggggcgtgcc tacgccacca tggcacggga
    53641 acagggcgcc tcgcgaatca gcctcggccg ggacgggcga ttgagttcgc ccggcttgca
    53701 gcaggccctg ctgcgtggcc tgctggcagg cggccttgat gtggtcgact tggggctgtg
    53761 cgcctcgccg ctcctgtact tctcattgtt caccttgccg gtggacggcg gcatcatgat
    53821 caccggcagc cacaatgccg ctgaatacaa cggctttaag atctgcatcg ggaaagaagc
    53881 gattcacggg gaagagattc agcggttgcg gcgggtcatg gaggccggtc aatttaccac
    53941 cggttccgga cgactctcct ctcatcccat catcccggac tacttggagc atctcaaaac
    54001 gaatttttcc ggcgtgcggg cggaccatct ccatgtcgtg atcgattgcg gtaatggcgc
    54061 ggcttcgttg gtggccaagc aggcccttga gcagatgggg tgtcgcgtga ccggcctcta
    54121 tgacgagttg gacgggcgat tccccaacca ccatcctgat cccaccgtcg tggaaaatct
    54181 gcaggatctg atcgcgacgg tgcgggagaa aaaggcggat gtcggcatcg gctacgacgg
    54241 cgatgccgat cggatcggcg cgatcgacga gcggggagag attctttggg gtgatcgcct
    54301 catgattgtc tatgcacggg atatcctgac ccagcgccc
//
LOCUS       DAORRQ010000024        54187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_34_length_54187_cov_133.519803, whole genome shotgun sequence.
ACCESSION   DAORRQ010000024 DAORRQ010000000
VERSION     DAORRQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 54187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54187
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34_length_54187_cov_133.519803"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="PK224_09535"
     CDS             <1..1554
                     /locus_tag="PK224_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRB16019.1"
                     /translation="EGVATAEVLKVGASSDAGAGGRVRLLLGAAALGGGFKFAEGGLK
                     LWSESLEGAVQLGRSTLYGGLNLSPALVAVGYIIGLNTAVVVFFGGAIGWLVLLPLYQ
                     AIIGSPEGLIGVAAAKATWSGQIRYIGIGAMLVGGVWTLFQVRGPIWQSLHQLLALYG
                     ARNQSDGQSELLRTERDAGVVWLIGLTVAALVPMVLLYQGLLNHNLWGGIGLTLLMVV
                     TAFLFSAVAGYMAGLVGSSSNPVSGVTIATIMLASLLLLGILGKGNPAGPAAALLVGA
                     VVCCAAAMGGDNLQDLKTGHVVGATPWKQQVMQVIGVATGAVVIVPVLSLLQAKYGIG
                     EVTAVHPHPLSAPQATLMANLANGVFGGSLPWHLVGVGMVLGMLVIGLDMRQARRNAA
                     LRFPVLAVALGMYLPLKLSATILFGGLLAEYVRAAGKFSDVPTEDRGLLCAAGLVTGE
                     ALVGILLALPIALSSIWPSLSGDPFQLFADPPLGGWPGLAALIVVGLLLVRAARSAGQ
                     KDSATVHRH"
     gene            complement(1593..2063)
                     /locus_tag="PK224_09540"
     CDS             complement(1593..2063)
                     /locus_tag="PK224_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB16020.1"
                     /translation="MPNVGTLIRAWRISQKCSEQALAERAGITASVLEALESEQADPP
                     ASTLEALAGALKIPLPWLFIHPTDLDLLCKDDNDEPAPLATLTGADPVLERILVAAGH
                     DRSLYVLLTALMQSSDPKLLRAAEVSLRSLVKQSKQATVPWQSRPPGHFEPPSD"
     gene            2239..2580
                     /locus_tag="PK224_09545"
     CDS             2239..2580
                     /locus_tag="PK224_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16021.1"
                     /translation="MLGTDIRGIMAEEEEVQRRQEALQSLMSMREKLLRESLEARIKR
                     ARGTGDWTNLSPAECANIYKEERVHLRAQLERLKAERDRTRGKLSALKRAKVRAQRIR
                     AAEAASGKKRK"
     gene            2649..3467
                     /locus_tag="PK224_09550"
     CDS             2649..3467
                     /locus_tag="PK224_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRB16022.1"
                     /translation="MTSSPRTVSRNFLSHIRDVVKTKRTRDRERVFVLEGTKPILELL
                     QSAPQHIVCLVVTPTFLERQPPEVAQQIVSSGCTVHSCPEHQLAQLSDVETSSGVLAI
                     VHQPTWNQSAILAQPKIFGLYGDMLQDPTNMGTIIRTAAGLNVSALWLAPHSVDVFNP
                     KVVRATAGALFQLPIFPHTSLEELQNLDCVIMAADAGTSEGCVPIRAIRSIPARTVLA
                     IGSESRGLSEPVLDAATVRFTIPLKPGVESLNAAAATAIALFQLSGLPVEGLKA"
     gene            3671..5032
                     /gene="radA"
                     /locus_tag="PK224_09555"
     CDS             3671..5032
                     /gene="radA"
                     /locus_tag="PK224_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRB16023.1"
                     /translation="MKAKTTFHCQACGHQALRWLGRCPDCGGWNTLKEERLPAAPKGR
                     QGMPKTAMAVATPISDIEIVGEPRHSTGMGEFDRVLGGGVVPGSVMLIGGDPGIGKTT
                     LLLQALPLLAAPGEQVLYVSGEESPRQIKMRGQRLGIDGKHLLILGETSLEQILKAIQ
                     EIQPAAVVVDSIQTVYTEQLTSAPGSISQVQEVAGQLMWFAKRSNVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKSHSFRILRAVKNRFGSTNEIGVFEMKDGGLEEVSNP
                     SELFLAERPQRSTGSVVVSSLEGTRPILVELQALVSGTNYPMPKRMANGVEPNRLSLL
                     LAVMEKRLGMHLSGQDVYVNVVGGIHIDEPAIDLGIVAAVTSSLRESPIDFTTLVMGE
                     VGLGGEVRAISQAELRIREAAKMGFKRCLLPERNVAKMEPVEGIELIGIHEVGDALDA
                     VLA"
     gene            5016..5900
                     /locus_tag="PK224_09560"
     CDS             5016..5900
                     /locus_tag="PK224_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16024.1"
                     /translation="MQYWREAKGETVEVTRKTASLGTVVVLLCLSVLSGCALFGPAET
                     IRLKKATAEQLTALLQEQAGEVRTIKGLFSAKITGGILPIGQRVQGTVFYQRPNAIRL
                     RGFSAVGSEIFEFVQIEDQFKLRLPTMGREVSGRPSEADQLGQLMRPFQLSVWAMSGI
                     IGTQVVGPDESVRLTEDGSRYRLDVVTAPGLNGTAPFVARRIWFDRRNLLVVQEERLS
                     PSGDVEATMQFDDYRSVGGVVEAVSAVNGQAPTADKRIMRPFAIRMTDGQGSGSLQVT
                     FHELVPNEPIKPSELGRV"
     gene            5897..6721
                     /gene="tsaB"
                     /locus_tag="PK224_09565"
     CDS             5897..6721
                     /gene="tsaB"
                     /locus_tag="PK224_09565"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRB16025.1"
                     /translation="MKAKPARHLLAIDTATAWQSVALLRDDQVLALIEQDAEGSHARS
                     LMGAIDRLLRGAGLSLKDLQALAVSIGPGSFTGLRVGLATMLGFRAVLGTPIVTVPTL
                     EAMAWNLRDVKGVLVPVLKSRHNEVYWAAYEWLPGTGLRTHIAEQVGPPASVARALQG
                     AKACTLFGDGWQAYEKDIREAVEAAGGQVREVRPEQQRPSAVSVGLAGRQRLGAGQVA
                     GPELVPCYVQRTEAEVKFDEQQGVSALERRRQRVASKLAQGRGKRGRTDADPQPRK"
     gene            6749..7249
                     /gene="rimI"
                     /locus_tag="PK224_09570"
     CDS             6749..7249
                     /gene="rimI"
                     /locus_tag="PK224_09570"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRB16026.1"
                     /translation="MASDSVLIEPATAEVLDEVFAIEQACFSAPWTRKMLAAELSGNQ
                     FAQFLIAKCPDPGSGAFVVVGYFCFWIVFEEVRLMNLAVLAPFRRQGVARRLVCAALR
                     AGLERGASRAMLEVRASNQEALTLYHRLGFRQTATRTRYYVNPDEDAVLMEMAPLQLG
                     PLCERS"
     gene            7318..7533
                     /locus_tag="PK224_09575"
     CDS             7318..7533
                     /locus_tag="PK224_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HRB16027.1"
                     /translation="MQTETAITERLRRSNTEFRALEESHHRLDAELADLQKRHVLTPA
                     EEVLKKQLQKEKLATKDKIAEFIRSSR"
     gene            7639..8409
                     /gene="tatC"
                     /locus_tag="PK224_09580"
     CDS             7639..8409
                     /gene="tatC"
                     /locus_tag="PK224_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRB16028.1"
                     /translation="MLAPLAAHIQSLKKRLLIIVVTLAVAFATAFAYSSEMVAWLNRP
                     FPNQLVFYGPTEALFASIKVSFLAGIILSLPMVFYQCWKFIEPALLPKEQRWAIPLFL
                     LAAFLFALGLVFCNLVILPLVIDFFVSFGMDRDITPALGVGTYIDFNVKFLLIFGCAF
                     ELPLVLTLLSRVGVVSAAVLAHYRKHAIMAALIISAIVTPDATLFTMLLMAVPLMVLY
                     EIGIIGAKIFGRAAGPAPDMNLPLDPDIPIGTAGHRVR"
     gene            8445..9521
                     /locus_tag="PK224_09585"
     CDS             8445..9521
                     /locus_tag="PK224_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16029.1"
                     /translation="MLRHMLVLTAFTLGGLQGFAHAASFPGGSEGGAPLNLEQGHPGQ
                     EPGASADIGNTSIQALTAGSGGVIYAGSFGMGIFRSADRGGSWTSANQGLTDPFVLCL
                     TTGKDGAIYAGTFRGGVFRSRDGGKIWQPVNKGLKRLEIKALMMDAKGLYAGTGDGVY
                     LLNAAQDQWKVVSTGLDEILVHALARTDDGTLYAGTSGKGVHSYKERSTGWTRLRQGL
                     RDHEGLVENFIRVLAVDKEQGVYAGTFDGGVFRSGDAGKTWSPISRALPNDSIRGLIS
                     NERGVYVATGRGIFKTVDKGRQWVPLNKGLSNLSVQVLIEGEKGGFYAGTSSGVFRSD
                     DDGLTWTAVSQGLEGESVAPFKFN"
     gene            9539..10048
                     /locus_tag="PK224_09590"
     CDS             9539..10048
                     /locus_tag="PK224_09590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRB16030.1"
                     /translation="MTAQAGSPVATISVTSKNEPWGEIVLRLFPDVAPGHVKNFVDLA
                     KKGFYNGTTFHRVIPGFMIQGGDPNSKNPDRASHGMGGPGHNVKAEFNSKPHKRGTLS
                     MARANDPDSAGSQFFICVNDANFLDWQYTVFGEVQSGLEVVDKVVGSKRDGRDNPLER
                     VEMTVTITE"
     gene            10148..10804
                     /locus_tag="PK224_09595"
     CDS             10148..10804
                     /locus_tag="PK224_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16031.1"
                     /translation="MTVFAAAALAAAGCAIPQVPSRTVYEDPVNFVRLELDANVLPEW
                     PPGHFTHPAQFSHDQVRRVLMGLTVQEHQASIQRWISGDSIRLPMFRDGEIAILVPQL
                     VEALRLARENERVTYYLSQPQTSVKRIITSGGLYVRGTELHFILGNWQTVYGIPAYGM
                     IYDRRYPMNPIVSKGFDLFFDLDQAMVIQSTSIWDWLLANSKDELVIDLARVFPGQPV
                     "
     gene            complement(11074..12507)
                     /locus_tag="PK224_09600"
     CDS             complement(11074..12507)
                     /locus_tag="PK224_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB16032.1"
                     /translation="MRRLLPHTTLLTLTTLALTALLASASRAADAEQPLTLPHGSFLG
                     VQRAVDIAVKNHPMLLEGAANLKASEARTEQARSLYYPQVYANANTVAGAGVSNPRFL
                     VGGGLLRENQTTFTGGVIANQRIYDFGYTSNLVESSKLAERAQGQDVSARRALVLLYV
                     QRAYLNSLKRRRLVQIAEETVRERGIIAGQIETLYRQQLKSKLDFDLAHVELVNAQSL
                     LVRSRNDLKASYADLNRTMGIAGADDYVLEDISVEVRPQKTLESLINESLSHPEVKRA
                     KEQTASADARLTATKRQYLPTVSAIASGGTFDPFDARQNQQTGGWWMAGAMVSMPLFT
                     GFLIENQVVEANANRQAASAATSSIEQALTQQVTNAYLDTLTFAQQITLAEEQVKTAQ
                     EALQLSKQRYKLGLGTVVEVTQSEVAVTAAQTRLAETQFDYKIAEVTLAYTSQGDDTG
                     RILLLSRATQPAPDTVSRLEGPRTPAN"
     gene            complement(12504..13802)
                     /locus_tag="PK224_09605"
     CDS             complement(12504..13802)
                     /locus_tag="PK224_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB16033.1"
                     /translation="MTEQVVPPPLVKRPNRTPLWIGTAVLLILAIGGYVYWQGDEPAT
                     PSAPARPAATAQSEPPAGPTPSELDSTPVDVQVTKPTRRDLVYTVKLPANVSPLYQTT
                     LYAKVAGYLKWIGPDKGDAVKKNEVVAVIDAPEVEEQYQQAVSDYKIKKLTYERLAKV
                     WKESPDVIAKQDVDVAEAAYQGARHLMEQRVVMRDYTKVRAPYDGIVTARFADPGSLI
                     QIATSSATSAIPLFTIMDLNTVRVYANVPQDDSPWVVPGKTSATVKVTELPNRSFSGV
                     ITRSTLALDPATRSLLVEIDLPNTDHALRPGTFAEVALGLREIPQALVIPPQAVISTP
                     KGKSVFIVEEGKAKSVAVQTGISDGRWIEITSGLQGHEEVVAVGKRRLLDGMPVQGSP
                     FNLPDAKLSQQKFERRVPGGTPLPPTSAPNGTSQKQGERP"
     gene            complement(13802..17071)
                     /locus_tag="PK224_09610"
     CDS             complement(13802..17071)
                     /locus_tag="PK224_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB16034.1"
                     /translation="MLTRAALKNPYAVFAICMIALILGAVSYQKMRVDIFPEIKLPSI
                     LVTTFYRGLSPSEMEGAITLKMEQRFVEASYVEHIESQSLSGMSYIKVFFQPDYGIDS
                     AQSELTSLAYSIIRLLPPGVYPPSVYKFGVSSLPVGYLSISSDTLGPKEIRDLAYFNV
                     RQQIATIPGISFGPPLGGKVRQITVFLDQQRLLARGVSPSEVVNALNAQSAIIPAGDI
                     KIGDLDYYVYSNSLVDVVDKINDIPIKIVNGTPVLIRDIGTAADSAAVQTSIVRVNGR
                     EATYIPITRQEGANTLEVTDGIRAKLSKLTEIPAGTTVKFLYDQSLYIRQAIANLQKE
                     GLLGAGLAGLMIFIFLASVKAALVVGLAIPLSLTAALVALYLTGQSVNLMTLGGLALV
                     IGTLLDNNIVVQENLHRHLEMGKDGRSAAEDSAMELTLPILVATICILIVYLPIMFFT
                     GIVKYLFVPLAMTVAFAMLADYVVSMSVTPVVLAWLYQAGHAKSSEHEASADEGWFRY
                     VLAIYEPLLTAGVRFKPVVIGLAALALVATAVFLLPRLHTEFFPKVDAGNFTMLVMAP
                     EGSRIEKTTAIVGQIEQLVHDTIPKGDLEEVISNTGLYFGDAARFAPNTGNHTAFVLV
                     NLVTGHQGHTEDYIADLRKKLKTSLPGVEIAFQTGGIISDVLNFGLKAPIDIQVKGPS
                     LEIIRPVAEQIQQRIAQVPNTVDVRIKQGKSYPELHIDVDRTKAAYYGINQNRVVVDV
                     ITGISSNLALSPNYWLDPKTANGYFLLAQYPEQSLTTTEDLLNIPIIGARTPLLPTAS
                     LTGGGMQGSTLALQNTPFAGRQMDMASGYYASGDDRRGPPVMLRDVATLNFKTGPDSV
                     DHYDLSRLINVLVTPVGNDLGHVAKDIEQALAGVPLPKDVTVQLRGEVANMRGAIQNF
                     ALALPLAVVLIYLVMVALFRSFIDPLIILVAVPLGWIGTVLTLHLTNTSVNVESMIGT
                     LMMMGIVVSNSILLVDFANRMVRHGATAEHAVLEAGRRRIRPILMTALATILGLLPLA
                     LGFGEGNETMVPLARAVVGGLAVSTIMTLLVVPIMHSLVLIRRERPPVSSTAPATLEE
                     I"
     gene            complement(17065..17493)
                     /locus_tag="PK224_09615"
     CDS             complement(17065..17493)
                     /locus_tag="PK224_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB16035.1"
                     /translation="MPSSILLIDDSPGECELFRQALTQSGYKGRLEITDGSRAAMTYL
                     NDHVPSDEPGLILLDLKLQGERGVDVLKRLKQDARLAPIPVVILTSSDDAMDVRACYQ
                     AGANGYVVKPGQFDDLVSLSLHLWKFWLEHNCTRRMATSC"
     gene            complement(17707..18081)
                     /locus_tag="PK224_09620"
     CDS             complement(17707..18081)
                     /locus_tag="PK224_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HRB16036.1"
                     /translation="MSNIIEQIDKAIGAHGAWKVKLRQNIDGTLALVPAEVGVDNRCE
                     FGKWLYSLASTPAATDPYYKEILELHKAFHKVAATVVTKVQSGDKTGAEASIGLKGDY
                     TAASSKLTAKMMEWKKTAPKAA"
     gene            18424..19686
                     /locus_tag="PK224_09625"
     CDS             18424..19686
                     /locus_tag="PK224_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB16037.1"
                     /translation="MSEDGSVSIPSRGWRLIQTRDFGLLWWGQTTSQIGEGLNKVALL
                     WFVYELTGSVMKMTMVGLLQTIPPLLFGPLIGVYLDRLPKKAVMIWVDLLRAVLTVLI
                     PALYAMDMLSIEGLYGLIFLTSVVSTVFGPALVSAVPLLVRPSELMSANALIQGTNNI
                     GMLLGPAISGVMIALINAENVLFVNSATFLLSALCLMPIRFSIPHVAARDASSSVWDE
                     LKVGFRFVFGQQPMVLTLVIISSLYNLGVSAFVFILPVYAKEFLQVGPVQLGWLWSAL
                     GVGMLAASTWLAWKKQSDMQGRLRIVVSGMTIGGLAVCSLSLLETPLVAAAIVIIVGG
                     STAVLNPIVWAVLQEVTPEHLIGRVLTTFSVGSMASAMAGMTGFGWVADAIGPAASLV
                     GLGLVLLLTAAVAVSCVRRARPVLVAQA"
     gene            19848..21095
                     /locus_tag="PK224_09630"
     CDS             19848..21095
                     /locus_tag="PK224_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB16038.1"
                     /translation="MAQAHRWLLTRDFSLMWWSQVLSQVAEGISKLALLWFVYAVTGS
                     PLKTTVIGLLQTLPPILFGPFIGVIVDRFPKKTILIGSDVARGVLIGLIPCLVSVEHF
                     TVGSLYLLTFLYGVATAVFVPTLSSAVPFMVARPQFTAANALLQSTTSLGIIIGPAVS
                     GLGIAFAGSQDVLCLNAMTYFASAACLLPIRLRVTEATTADGDGWRPFIRHLLEGIRY
                     AFLTHRTVLILIVLASVYTFGSGAFTTLFPVFGRHLLGLGPVEVGYLWSWLGVGLLLS
                     SIGLIRMSGWDLSQRLSVITLSCALAGLALLGLTWTDDLRLAAVLVGCIGIGFGAWTP
                     IAWGIIQEVAPAGMVGRVMAVYTASATATSMLGMSAFGWAAESFGSVASLIGIGGVMV
                     ILAGGSAWFGKRIGVAVIGKADV"
     gene            21259..21417
                     /locus_tag="PK224_09635"
     CDS             21259..21417
                     /locus_tag="PK224_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16039.1"
                     /translation="MKSPGGKARASKFSKQVGAALRRAAKVARKTARMYGTPIYVWEN
                     GKVVAKKP"
     gene            complement(21593..21850)
                     /locus_tag="PK224_09640"
     CDS             complement(21593..21850)
                     /locus_tag="PK224_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16040.1"
                     /translation="MRQDIMKDESHRDTAESKHSDSTRQRSLTHDETKAAEAAFKGEP
                     FNPAWSAAAATVYAGITQAIEKRLMAVVVESEVTEECLAGR"
     gene            complement(22239..22475)
                     /locus_tag="PK224_09645"
     CDS             complement(22239..22475)
                     /locus_tag="PK224_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB16041.1"
                     /translation="MRSEEGMTTKTRRQYTDEFKAEAVRLVRDSARPVTHVARDLGIA
                     DHLLYRWRAEQQQAEGQGQTRQSARAEQAELAGP"
     gene            23661..24848
                     /locus_tag="PK224_09650"
     CDS             23661..24848
                     /locus_tag="PK224_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16042.1"
                     /translation="MEFGDQSMRPSLLSAIFAFFILTGICAFTQSGFADEQPVSASHQ
                     PFQDWLIEKIDADPSEWDRLRDLILPISKRLAYDAHQKEVVILPYDIPDVVLRPMSAD
                     AREDPQLRPFIGAAGEPPRLRPFLSSGGMWNWVTLNPFLPGHYDVTLGVLNEEGMPWS
                     FSSEAAEVVADASRDPDLFEWNCPAAHAQNPIASTVTSRFDFTRGKEGFFTWIKGRVN
                     EIREAQVAGDDRLALYHLGRVLHSIQDLASHRGMDNAEHSWLDHQGEGPDEDTLINIP
                     LAKTLTRDFLEHLSEISPSINYALLRNPRNALSTWGYKEVKDRLGFSRQLTKKEYDAF
                     KKLGSQLPASLNGLTGVEFEVAVKQLKNRWFTPADWRKMISEIKEHSLKLPLSESVLT
                     TCH"
     gene            25024..25674
                     /locus_tag="PK224_09655"
     CDS             25024..25674
                     /locus_tag="PK224_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRB16043.1"
                     /translation="MLVSHDGYILTAKHVIEDYYKSPESTSILVRILDGNLTLRPEQI
                     AEAVSRDPNVDMALIRVTGEKFPFLRIGNSSMVKIDEQLVAFGFERSGGKWLTKSGRR
                     NGLNWEVDIPLFPGSSGGPVVKSGCDMLVGIVVGGLEDPITKALIPGRSQILPSQFGA
                     FVLGYGASTQSAKLCDSMVESPLHSPTPNRPPTSPCKEITVSETVNGVTTWAKKCL"
     gene            25713..26159
                     /locus_tag="PK224_09660"
     CDS             25713..26159
                     /locus_tag="PK224_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HRB16044.1"
                     /translation="MLISRLVIVGLCICLLLTAAVSLADQTDEARAVAMTILQKLEQK
                     KNSEVWESHVSNWFKERTTKPAFLANMTLIQSQLGGIGSHRELVQQNLANSSPQVGYR
                     GEVYSFMFATTFPSANVYEMIVMIKEDSTYRLSGIQYTPNPNVSSP"
     gene            26440..27189
                     /locus_tag="PK224_09665"
     CDS             26440..27189
                     /locus_tag="PK224_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16045.1"
                     /translation="MTLFRSVAMLVSLVLVPLTVGAAPPPEPTVEYSADSTMETEGGM
                     TMKSRVYHTPTKQRMEMGGADGSVTIIRKDKKVVWQVMGDMYMEMPLDQSNAPDMGGF
                     DIQEQTEVGEETVNGLKTTKTKIIATKPDGSKFGGFFWTTKEGVTVKMDLLSKDGDKK
                     MRMASELTNVKIEKQNPALFEVPAGATKNDMGAMMGQGGMPNMEEMMKNAGQERAREK
                     RSSRSNSGSKSQESETPAAEVNKMLKGLFGR"
     gene            27202..28179
                     /locus_tag="PK224_09670"
     CDS             27202..28179
                     /locus_tag="PK224_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16046.1"
                     /translation="MRTVRILSCGLLLLAVTVSPVRADDPCLGDEEEKSAKAAVAALT
                     KAEQAGRPAELFVTYRSILGNDCLDRYDRTALSRAKSALPKLGRELAKAAEVKGLWYS
                     PDPVRGDGKTSAFRYFETIGDYADANRVMMKALQAKPEDLALFTTAWGVDEGRWVVPD
                     PKTAERQPYVSPQAYRQELQKIASSTADRLMKAEEQDAKGLSGSAVEVATSAMKSLQK
                     LRTAAGWMKFLSVGDKTARERAEQRGDTITARSDSTFTQANAVMYYEFAGSPKSKDKL
                     TQVKKKMDESSRALEKSGEKVKGAFAEQSQAEQKQFDKKKADLEKELGF"
     gene            complement(28238..28684)
                     /locus_tag="PK224_09675"
     CDS             complement(28238..28684)
                     /locus_tag="PK224_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRB16047.1"
                     /translation="MDAIVRGLVVYLFLMILFRIAGRRTLGSITNFDFVLLLIISEAT
                     QNAMIGNDYSVTNGFLVILTLVGLDIVLSYMKQRFPAMERYLDGLPLILVDQGRPLKD
                     LMYRVRVDEQDILAAAREKHGLERMDQIKYAILETNGMISIVPKTG"
     gene            complement(28697..29131)
                     /locus_tag="PK224_09680"
     CDS             complement(28697..29131)
                     /locus_tag="PK224_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16048.1"
                     /translation="MERFGQVLIACCLIAALLGLFGHHPLASVTQQTADGGLSIHYDR
                     FARLEKSGDIGVTVEPLKDRDGVVRVWLDQEYLDASKLTSISPTPIRVEARNGGRVFV
                     FQSDGSRFTATLSVQLDAVGTVHGSVRVDDGEPLSVTQFVWP"
     gene            29450..29950
                     /locus_tag="PK224_09685"
     CDS             29450..29950
                     /locus_tag="PK224_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16049.1"
                     /translation="MSKLFIYLCMVVAVLGMTSQVGRADEGSPNSHHQELQGVVVEKG
                     GALAVKVPNGATYQLNENRAERHGHALPKAGDEVTVVIDENNMVLEVHPKGTEGKHRF
                     VTGEVVSIGTMQNEITLKTAKGEQKFPLEKQVLSASIKDGARVRAELNEAGHIIDVHP
                     EKSGAH"
     gene            complement(30026..30529)
                     /locus_tag="PK224_09690"
     CDS             complement(30026..30529)
                     /locus_tag="PK224_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16050.1"
                     /translation="MRRYRQVVMWMAGALLLQGCVGVGAWTLGSRTESSDHPKMDRTR
                     GVLDMRNGEPKGTQKTVAELRAQWGEPDRVESLEGGKEEWIYKTDGLRWSGLVLYVVI
                     VPLPAMLPVGSQYVSFLIHDGRIENVTRADWAFKAGAYCGFFGMMYGGLGCGTGTFEE
                     QQSVATH"
     gene            30709..31089
                     /locus_tag="PK224_09695"
     CDS             30709..31089
                     /locus_tag="PK224_09695"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB16051.1"
                     /translation="MAPRRYVRTYYRFPLNYPVIFGGAPFVGEGVLTNLSLRGCSVMC
                     DREVLCGSDVRVSVLLDHQPPALPIDLGTIKWVKGHQFGVEFVRLPLEAQQRLNRTLR
                     TELIEWLKSRQNSSDLPETSNPDN"
     gene            31307..32386
                     /locus_tag="PK224_09700"
     CDS             31307..32386
                     /locus_tag="PK224_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16052.1"
                     /translation="MNILHLGNPFFLQDFRQLGHDVKWAAYDRTADFVLSPYVESLHS
                     LSAQFPARWFPDLVVVGDDSGPPKLLGLEALDVPLVWYAIDSHLHASWHQQYAAVFDV
                     ICVAQRDWVSRYRVDPDRQIVSWQPLFCHVPDDGDSGRLRDTPLSFVGTLNRQWNPER
                     VALIEGLQRRYPIAVGSGAYREPFNRSLMVLNQSAANDVNFRTFQAMACGALLIGERI
                     GNGFNDLFQDRTHCALYDRGNVEQVIEIVAHYCGRPAERDAVARQGYEAVMASHTSLH
                     RAQAILQVVATAPLQGQIRVRRSRQLQIQSSLVLVYESAERTHLQAAERTPEGPRKQY
                     LLTVAEQYQFLATTIRTQLGLQVAC"
     gene            32894..34006
                     /gene="recA"
                     /locus_tag="PK224_09705"
     CDS             32894..34006
                     /gene="recA"
                     /locus_tag="PK224_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRB16053.1"
                     /translation="MTEKDEKKRALDLALAQIEKQYGKGAVMKLGADDRPADVPAISS
                     GSLGLDIALGVGGFPRGRVIEIFGPESSGKTTLTLHAIAEAQKAGGVAAFIDAEHALD
                     LTYAKKLGVQTDDLLVSQPDTGEQALEIAETLVRSGAIDVIVVDSVAALVPRAEIEGE
                     MGDSHMGLQARLMSQALRKLTAAISKSQTTLIFINQIRMKIGVMFGNPETTTGGNALK
                     FYSSVRLDIRRIESIKDGQDVTGSRVRVKVVKNKMAPPFKQAEFDIMFAEGISKSGEL
                     VDIGVEKRVVEKAGAWYSYKGERLGQGREAVRDFLKANPAIAKEIEGKVRDLAGLPSR
                     GTEKKVEGKEAKDEKPERKPEGRQDDKRGHSARVTT"
     gene            34046..34504
                     /locus_tag="PK224_09710"
     CDS             34046..34504
                     /locus_tag="PK224_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRB16054.1"
                     /translation="MKPTPDYLTLAIKYLARTDRTVAQVERYVLEKGASRAEERLVVR
                     ELERRGYLNDQAYAIRWAETRLSRHPMGRERLKAELLSRGFEDSVAERALRQAYRSIS
                     EQELACQALEGRASRTRPAQWVRFLRQRGFDDDTIQQVTQVDLETGLDEL"
     gene            34513..37131
                     /gene="alaS"
                     /locus_tag="PK224_09715"
     CDS             34513..37131
                     /gene="alaS"
                     /locus_tag="PK224_09715"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRB16055.1"
                     /translation="MNDLRRAFIQYFEQQGHRAVPSAPLIPQADPTLLFTNAGMNQFK
                     RVFLGEETRAYNRAVTVQKCLRAGGKHNDLENVGYTRRHHTFFEMLGNFSFGDYFKED
                     AIRFGWEFLTSVVGLSKDRMWITIFREDDEADRLWRKIGVSPSRIVRCGEKDNFWQMA
                     DTGPCGPCSELHFDQGPSVPGDATPNGEGDRVIEIWNLVFMQFNRDSAGTLNPLPKPS
                     IDTGMGLERLTAVAQGRLSNYDSDLFAPLLAAIGSRAGAQYGVLEQADRSMRVIADHL
                     RAITFLMADGVLPSNEGRGYVLRRILRRAARHGRLLGITEPFLHELTATVVEQMGEAY
                     HELRPAAGTVAEATRGEEERFIATLDQGLPILNDMLTKVRASGQNMLSGTEIFKLYDT
                     YGFPMDLIAEACREQDIRLDETGFEAAIEEQRTRARKTGGFEGETARPALSEVAARVG
                     TTAFVGYEHLNSEGVVQALLKGDRLVKDAREGDDIEVVLDVTPFYAEGGGQAGDQGLL
                     TGPDGRVEIRETTRPVPTLIVHKGVVTSGSIREGERLQLSVNRRTRHDAARNHTATHL
                     VHAALRDLLGPHVKQYGSLVAPNRLRFDFAHFRPLASRDIDEIESIVNEQVRQDQPVQ
                     TDVMGVQEAVAGGALAFFGDKYGDQVRVVHIDTFSKELCGGTHCRRTGEIGLFRIVSE
                     SGVAAGVRRIECLTGSGALDSLKRLEADVRELSDLLKVAPGEVVARTRKLSEQLKEKE
                     RELAEVKLKMASTSSGDAQAREIKGVQVHAQRTDGLDVNGMRALADQLRDKLRSGVVA
                     LGAANDGKVSLLVVVTKDLVGRLKAGELIKEMATEVGGTGGGRPEMAQAGGKNPEGLG
                     TALEKVFGLVQKALEG"
     gene            37136..37747
                     /gene="ruvX"
                     /locus_tag="PK224_09720"
     CDS             37136..37747
                     /gene="ruvX"
                     /locus_tag="PK224_09720"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRB16056.1"
                     /translation="MKGQRILAIDHGSKRIGFALSDELGWTAQPLETFYRRNPDADIR
                     HIQDLVREHEVGQVLVGMPLRLDGEIGPAAKVVAEFIQLLEPALSVPVITWDERMTTC
                     AAEDLLIAADVGRRKRKGIVDRIAAAILLQSYLASLEQPATSQANDLNAPVETDPWVF
                     DEPRVDDAEESDHGSGPGGSDVRGSRGISGAALGSKPRRERTS"
     gene            37632..38684
                     /gene="mltG"
                     /locus_tag="PK224_09725"
     CDS             37632..38684
                     /gene="mltG"
                     /locus_tag="PK224_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRB16057.1"
                     /translation="MMQKKAIMGLALAAVMFAALAGYLVLRWAQSPVASGPPKPPSHI
                     VLIPEGSTFQQVAALLKNEQLIRSRSAFLLLGKTRDIDRKIRPGEYELDASMSPQDIL
                     TKLLAGRVVLHPVTIPEGYSLTQIAEVLAAQQVTDTKEFTKLVRDRTFISTLGIEADS
                     LEGYLFPETYSFAKGTKAREVIKAMVDGLHRVWGTELQEQAARMKMSLHQVLTLASVI
                     EKETGAKHERELIAAVFHNRLRKKIPLQSDPTVIYGLPAFDGNIHKRDLSVMSPYNTY
                     RVQGLPPGPIASPGAHSLRAALFPAQASYLYFVSRNDGTHQFSSTLAEHNQAVEKYQK
                     QYFRKRARGNLVAHGA"
     gene            38759..39448
                     /gene="deoC"
                     /locus_tag="PK224_09730"
     CDS             38759..39448
                     /gene="deoC"
                     /locus_tag="PK224_09730"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRB16058.1"
                     /translation="MSVKSWNESLPRYLDHTVLRPEATKADVLRLCAEAREQGFVVIF
                     VPPCYVDEAVAAVAGTDVQVGIPIGFPLGGHSTHAKVTEAMEAVAHGARVLDMVINIS
                     RLKSGDYDVVRNDMAAVVQATPGVNHKVILETCLLTREEKITACRLAVEAGMDYVKTS
                     TGFNQAGATVEDVRLMKEAVAGRAKVKASGGIRDWKTTRELLEAGADRIGTSASLKIL
                     SEWRAALVAVR"
     gene            39504..40658
                     /locus_tag="PK224_09735"
     CDS             39504..40658
                     /locus_tag="PK224_09735"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRB16059.1"
                     /translation="MMTRIVLLVLDGFGIGALPDADVYGDAGCNTLQRLAAISKGLAL
                     PNFEQLGLGHLGQFQGIRPMVQPEGCYGTLGFSTKGKNSLSGHWEIAGYVIEEGERPC
                     ETFTTELANALEAALGQKTLGNCRAVTQEPIAEFGGQHQKSGMPIAWIDTAGTIFLAA
                     HEQVVPPEELYRLAREARKLLKGMVPIVRVVACPFVGQSGKFTATERRRDFAVEPPGL
                     TLLDHLSRASQLVIGVGKVGDLFSGRGVTRSVPLFQAEAVMDEVVGMFSKVPRGLIYA
                     SLPVINPDLHTTVSTLQQIDRRLRDLQESLKVGDVLIITGDHGFDCARPNPGHSREYV
                     PCLVTGPRLARGVNLGTRSTAADLGQTIGEALGATRLPWGDSFLDALQSR"
     gene            40704..41165
                     /locus_tag="PK224_09740"
     CDS             40704..41165
                     /locus_tag="PK224_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRB16060.1"
                     /translation="MSIDATLKSLGIELPAPPKPVASYVPAVLAGDLLFLSGMLPFRD
                     GQVAITGKLGQDVTVERGAEAARLALLNALAVVKQELGSLDRVRRIVRVVGHVASAAG
                     FVQQPAVINGASDLLVQVFGEAGRHARVALGAAELPLHAAIELELLVQVRP"
     gene            41409..43085
                     /locus_tag="PK224_09745"
     CDS             41409..43085
                     /locus_tag="PK224_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRB16061.1"
                     /translation="MQYIARLVLGVALFAGPVVSGEVRAEGPVLHPNAAVPGSTLSIT
                     GKGFGPYKSTKFNQVTFQGVPALIQRWEADLIEVRVPSQAANGPVDVIIGKKHVKAGS
                     FTRLQPAIHSLSPAEAEPGTILEITGEHFGNTAGPRDPNTIFGVNTVAVGDVTVRVRK
                     WRDDKIEVELPGNVQSGDVVIRMASSDPLPDGSCCAPVKQVVSNSMPMKVLASVRVDP
                     TSGPVGTKVVLFGKGFGTSRNPEDGVLFGGHLATVSQWTDTTIVVHVPLDAQTGPVVM
                     KRNGQERAIGTYTVQTPQATGLTPAEAPIGTLLKITGENFGFYSEAGSTPFNYIDFSL
                     SENTVEIGGVQAIVYRWGHDRIDVWVPFSAKSGPVVVKRAANAPKPDGTCCADKKVLE
                     TQVGNFTLVTPKIDSYSPTTGGLDEVVTIKGSGFGKFLKTAEPSKLITDSVYARVAPV
                     LGENVSRTEVLFNGVGAIVQSWTDTEITVKIPHRHSYGVGKLGEFNPDLTSGPLVVRR
                     GSWDLLPDGSCCTPKKWLTLEAGTFTIQPTGLPDASYWRNQSSENTHHQQ"
     gene            43097..44065
                     /locus_tag="PK224_09750"
     CDS             43097..44065
                     /locus_tag="PK224_09750"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRB16062.1"
                     /translation="MHILAVVSWLFVALLCAGTAEAASPESRATVAMQKSGSEATLLG
                     MFFHDPQLGWAVGSGGTILKTTDGGRKWKKLSSGTTSLLTAVYFQDARRGWVVGANGT
                     VRTSRDGGETWSVVFVSTQAPLYGIAFVTPQKGWLVGGNGTILQTSDGGENWTDQASG
                     TSAALHSIVLTNQQHGAIVGALGTILTTSDGGASWTPQVSQSSATFFDVAFADEVNGW
                     AVGNAGALFQTTDGGTHWIDRTLPCGRTCNKLTDLIRVRFTDAQQGWIVGEHGQILRT
                     TDAGFNWVEEASPVKRSLMALSFPHTTAGWAAGEGGTIISINPGRR"
     gene            complement(44085..44822)
                     /locus_tag="PK224_09755"
     CDS             complement(44085..44822)
                     /locus_tag="PK224_09755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRB16063.1"
                     /translation="MPAFFVSSLDIRETQITLTGDLCHHVRASLRVKPGEDLWLTDEQ
                     RRRYRVRVTHVSQQAVMTEILEQRLGPVETGPPLLLAQALLKGDHMDWVVQKASELGV
                     RTILPLVSRHGVVRPQPDRIAAQVARWQRIATEAAQQSEQWQPPEVLEPLESRRFFST
                     HKATCSLLLAERHEAAALARIPLPSLPSEQLTVMIGPEGGWAEEEITLALAQQCQAVS
                     LGSCILRADTAAVTALSIVQSRLGRLG"
     gene            complement(44857..45969)
                     /gene="dnaJ"
                     /locus_tag="PK224_09760"
     CDS             complement(44857..45969)
                     /gene="dnaJ"
                     /locus_tag="PK224_09760"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRB16064.1"
                     /translation="MAKRDYYETLGIERTASDDEIKKAFRKLARQHHPDLHTSPEQKK
                     SAEEKFKELNEAYEVISDQEKRRRYDAFGHAGGPQGAEGFDFGGQGGFGDIFNDIFED
                     FFGQPRGRARAERGNDLQYNLEVTFEESVFGKEAKLKIPRWEICADCKGTGARSATAI
                     KMCPACKGQGQLRFQQGFFSVSRPCGQCEGVGQIITEPCQACGGRQRIRKERMLSVQI
                     PGGIETGMRLRLANEGEHGVQGGPQGDLYVAITVKPHPHFRREGQDIVSELPVNLVTA
                     ILGGRVEVPTLKGNTFMKIPAGTQPDKILRIKGLGFPNLKGGHTGDQLFNVKVEIPTK
                     LNSKQKELLEEFAKESGYTKDTEGEGFLDKMKTFFE"
     gene            complement(46051..47982)
                     /gene="dnaK"
                     /locus_tag="PK224_09765"
     CDS             complement(46051..47982)
                     /gene="dnaK"
                     /locus_tag="PK224_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRB16065.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVGITD
                     KNERLVGQIAKRQAITNPENTIFSVKRLMGRKFRSKEVQEAMKRLPYKVVEADNGDAH
                     VELRGKRYSPPEVSAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDVSVLEIGDGVFEVKSTNG
                     DTYLGGDDFDERVMDWLVEEFKKDQGIDLRKDRMALQRLKEAAERAKIELSSSQESEI
                     NLPFITADASGPKHMVTKLTRAKLEQLVGDLIQRTIEPCRKALADAGVSAKDIQEVVL
                     VGGMTRMPKVIQVVKEFFGKEPHRGVNPDEVVAIGAGVQGGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTIPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPPAPRGMPQVEVSFDIDANGIVHVAAKDLATQKEQSIKITASSGLSKDEVD
                     KLVKDAQSHTEDDKKRRRVAEARNQADSLLYSTEKNLSEHGDKIGEDDKTKITEAIAG
                     LRKAMEGDDPAAIETATQTLTTASHKLAEEMYKKASAAAGAGAGADAAASSGDGGAHA
                     KTDEKVVDAEFEEVDKDKK"
     gene            complement(48072..48626)
                     /gene="grpE"
                     /locus_tag="PK224_09770"
     CDS             complement(48072..48626)
                     /gene="grpE"
                     /locus_tag="PK224_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRB16066.1"
                     /translation="MSQDDKNIHSIDNLDGSSEASSGTMEGVNELQQVLDAKSDECKA
                     LNEKYLRLAAEFDNYKRLAQRDQREQIKFGNEQILKELLPVVDNLERAIKSSKGSGSV
                     DALTEGVELTLKQLVGALTKFGVKAVESVGLAFDPATQQAVAQVPSDTIPENHVVEEY
                     QKGYLLQDRILRAAMVTVSTGAAN"
     gene            complement(48761..49531)
                     /locus_tag="PK224_09775"
     CDS             complement(48761..49531)
                     /locus_tag="PK224_09775"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRB16067.1"
                     /translation="MLLTIDIGNTNVVWGLFEDSTLRGHWRLATESRRTDNEYGILLL
                     NLLNNAGFTPEQITGCILSSVVPALTGTFDSLVQTYFHRTPVIVGPDTDSGLTLRYAN
                     PKEIGSDRIVNAAAAYARYRSDLIIVDFGTATTFCAVTKSAEYLGGVIAPGLGISADA
                     LFSRTAKLPKVEIIRPKTVIGTDTIGGIQSGLLFGYVGLVDGIVRRMERELGRTSTVI
                     ATGGLATVIAQETETIQEVRPFLTLEGLELLYHRNRLT"
     gene            complement(49567..50439)
                     /locus_tag="PK224_09780"
     CDS             complement(49567..50439)
                     /locus_tag="PK224_09780"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRB16068.1"
                     /translation="MGGPANDPHPADRLGIDRLQAGLHTHTFGQTLRYIPSTASTNAD
                     ALAYLQQPTGPAAAHGTVILADCQTAGRGRRGRAWHSPAQGNIYMSVIVVPEPGSTRI
                     GPWLSWIPLFSALAVSDCLSGCTGLAVSVKWPNDLLIGDKKIGGILCEQTTTADRTLA
                     IVIGIGLNINAAPDSFPEELRAGATTMAAEAGHRLDRAMILADLLLRLEQRMDRLFHD
                     GPSGMIDEFTRRCSTLGKTVRVALEENGIVQGIAESIGPDGCLCLRVTSDPSPTLPHR
                     LLEVRSAEVIHVRG"
     gene            complement(50423..51304)
                     /gene="nadC"
                     /locus_tag="PK224_09785"
     CDS             complement(50423..51304)
                     /gene="nadC"
                     /locus_tag="PK224_09785"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRB16069.1"
                     /translation="MNAIPHTQLRAVVQAALAEDLAHGDVTTSALFPCSIQARAAIVA
                     HQPITVAGVVVAREVFLAVDPSLRIVKSINDGATVKPDAEVIVIRGDVRSLLMAERVA
                     VNFLQRLSGIATLTAKFCAAIRHTETAILDTRKTTPGLRALEKWAVHLGGGRNHRFSL
                     GDGVLIKDNHLAVLRSTGVDVAGACRLARAGAPHGLRIEVEAKSLQEVKEALAGHADI
                     ILLDNMSPALVQKAVALIKKRALVEVSGGITLQTVADMAQTGVDFISVGALTHSAPAA
                     DLSMDLTGYRGSRGRTS"
     gene            complement(51301..54042)
                     /locus_tag="PK224_09790"
     CDS             complement(51301..54042)
                     /locus_tag="PK224_09790"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRB16070.1"
                     /translation="MSTPQLDKTYSPHDVEDRWYQRWIDAGLFHADSAHPGQPYSMVI
                     PPPNVTGSLHVGHALNNTLQDILIRWRRMQGRNVLWMPGTDHAGIATQNVVERQLQAE
                     GTTREALGREEFLKRVWQWKEKSGGTIISQLKRLGASCDWERERFTMDEGLSEAVREV
                     FVRLHADGLLYRGERLINWCPRCLTALSDIEVEHEEITGKLYTIRYALADDPAQVLLV
                     ATTRPETLLGDTAVAVHPEDERYRHLIGKQVRLPLTTRAIPIVGDSILVDREFGTGAV
                     KITPAHDFNDFEAGERHGLRRIKILDIHANLRLEGALVESEVAHIVEGLPVAKARPKI
                     EQLLTDQGLLEKSEPHKMALGKCYRCKTVVEPLLSDQWFVKIKPLAEPAIQAVEDGRV
                     RIIPEAWKNNYLGWMRDIKDWCVSRQIWWGHQIPAWYCETCYGSPFLRRSSDGAPLIP
                     SDATVIVAKTTPATCPKGHSDALVQDPDVLDTWFSSALWPFSTLGWPKQTPDLKTFYP
                     TSTLVTGLDILFFWVARMIMMGLKFMGDVPFRDVYIHALVRDAEGQKMSKSKGNVIDP
                     LHVMEQFGTDALRFTMAAMASPGRDVKLAEERIEGYRNFTNKIWNAARFLLMHLDGER
                     LEMPAAQRSFPDRWILSRLNATIGTVTQELEQYRFDRASSAIYQFIWHEYCDWYLELI
                     KPALKDQASEQAKSTRQTLAETFETMMRLLHPFMPFISEEIWQTLPHEGDSIVRQPFP
                     VAQPEWSSHDAEGLFALLDRSVSTMRMGRSLLNYPPAKVLEFHVFGPDMSNPAPLDYL
                     RDHIAHLVRGSVQVHAAWPTQRTLRLVTDGLSVGVNVEGDVELQKVLARIKKQLEDGK
                     KEAERLEGKLGNKEFVAKAPPEVVVEQRQRLSTLYKDQAILESSDAQIRSMLAELGP"
BASE COUNT        10487 a        14959 c        17031 g        11710 t
ORIGIN      
        1 gaaggggttg cgacggcgga agtcctgaag gtcggagcct cgtcggatgc cggtgccggt
       61 ggccgggttc gcctattgtt gggcgctgcg gcgctgggtg gcgggttcaa gtttgccgag
      121 ggaggattga agctgtggag cgagtcgctg gaaggggccg ttcaacttgg gcgttcgacg
      181 ttgtacggag ggctgaacct gtcaccggcc ttggtggcgg tcgggtacat catcgggctc
      241 aacacggccg tcgtcgtctt tttcggcgga gcgatcggct ggctggtcct gctgccgttg
      301 taccaggcga tcatcggttc gccggaggga ttgatcggcg tggcggcggc gaaagcgacg
      361 tggagcgggc agattcgcta catcggcatc ggggctatgc tggtgggtgg ggtctggacc
      421 ctgtttcagg tgcgtggccc gatttggcag agtctgcatc aactcctggc gctgtacggc
      481 gcccgcaacc aatctgatgg gcaatcggaa cttctgcgca ctgagcggga tgcgggagtc
      541 gtctggctga tcgggctgac ggtggcggca ctggttccca tggtgctgtt gtatcaaggg
      601 ctgttgaatc acaatctgtg gggcggcatc ggattgaccc tgctcatggt tgtgacggcg
      661 tttctctttt ctgccgtggc tgggtacatg gctggtctgg tcggcagctc gagcaatccg
      721 gtttcaggtg tgacgattgc caccatcatg ctcgcatcac tgctcctgct ggggattctt
      781 gggaagggca atccggccgg tccggctgcg gctttgctgg taggggcggt cgtctgttgc
      841 gccgcggcga tgggcggcga caatttgcag gatctgaaaa ccgggcacgt cgtcggtgcg
      901 accccgtgga agcaacaggt gatgcaggtc atcggcgtgg cgaccggcgc ggttgtcatt
      961 gtgccggtgc tgtccctgct gcaggcgaaa tatggaatcg gtgaagtaac ggccgtccat
     1021 ccccatccgc tctcggcacc acaggccact ctcatggcca accttgccaa tggagtgttc
     1081 ggcggttctt taccctggca cctggtgggc gtggggatgg tgctggggat gctcgtgatc
     1141 ggtctcgaca tgcggcaggc acgacggaat gccgcccttc gtttccctgt cctggcggtg
     1201 gcgcttggta tgtacctgcc gctcaagttg tcggccacga tcttgttcgg tggtttgttg
     1261 gccgagtatg tacgggccgc cgggaaattt tccgatgttc cgacggagga tcgaggtctg
     1321 ctgtgcgccg ctggtttagt cacaggtgaa gcgctggtcg ggatcctgtt ggcgcttccc
     1381 attgccctca gttcgatctg gccgtcactc tcgggagatc cgtttcaatt gtttgccgat
     1441 ccgccgcttg gtggatggcc ggggctcgct gcactgattg tggtggggtt gctgctggtg
     1501 cgtgccgccc ggtcggccgg acaaaaagat tcagcgactg ttcatcgaca ctgatggtgt
     1561 gggacggtgt agaggtggtc ggcgaggggg cgctaatcgc tgggtggttc gaaatgtccc
     1621 ggtggtcgtg attgccaggg gaccgtcgcc tgcttggatt gtttcacgag gcttcgcagg
     1681 ctcacctctg cggcgcgtag gagtttaggg tcgctgcttt gcatgagcgc cgtcaggagg
     1741 acgtatagcg accggtcatg tccggccgcc acgagaattc tctccaggac gggatcggct
     1801 ccggtcaatg tggcgagggg ggccggctca tcgttatcgt ctttacacag caggtctaaa
     1861 tccgtggggt ggataaagag ccaggggagg ggaatcttga gtgcgccggc caacgcttcc
     1921 agggtggagg ccggaggatc ggcctgttcc gattccagcg cttccagcac cgaggctgtg
     1981 atcccggctc gttctgcgag cgcctgctcg gagcactttt gcgagatccg ccaggcgcga
     2041 atgagagtgc caacgttcgg catggacgga tcttactgta tccctttccc ggtctgcaac
     2101 ggcgcatttt cgaggctcat ccgatacttg cctgtaacat caacgtgtta cgatccattc
     2161 tcctaggtag aacaaagccg ctcaaatcgg gtagaatgag gcgttctaat tgttgtctcg
     2221 agcgaggagg atcagccgat gttaggaacc gacattcgcg gcattatggc cgaagaggaa
     2281 gaagtccagc ggcgacagga agccttgcag tcattgatgt cgatgcgcga gaagctgctg
     2341 cgtgaatcgt tggaagcgcg aatcaagcgg gcgcgtggaa cgggagactg gacgaacctg
     2401 tcgcctgcgg aatgtgccaa tatttacaag gaagagcgcg ttcaccttcg tgctcagctg
     2461 gagcgattga aggccgagcg ggatcggacc cgcggcaagc tgtcggcgct caagcgagcg
     2521 aaggttcgtg cgcaacgcat tcgcgcggcg gaagcggctt ccggtaagaa acgcaaatag
     2581 gtcgatgtcg gggaggcctt gcctgttcag taaggcctcc tcgcagtgtc gcagactttg
     2641 ggtaactgtt gacctcctca cctcgcaccg tttctcgcaa ctttctctcc cacatacggg
     2701 atgtcgtcaa gacgaagcgt acgcgtgacc gcgaacgggt cttcgtgctt gaaggcacca
     2761 aacccatcct cgaattgttg cagtcggcac ctcaacacat cgtctgcctc gtcgtcaccc
     2821 ccacgtttct cgaacgacaa ccgccggaag tcgcgcagca gattgtcagc agcgggtgta
     2881 ctgtccacag ctgtccggaa caccagttgg cccaattgtc cgatgtcgaa acctccagcg
     2941 gcgtgctggc catcgttcat cagccgacct ggaaccagtc ggccatactg gcgcaaccga
     3001 aaattttcgg cctctacggg gacatgctgc aggacccgac gaatatgggg acgatcattc
     3061 gaactgcggc gggactcaat gtgagtgcct tgtggctggc accccactcc gtcgatgtgt
     3121 tcaatccaaa agtggtgcgg gcgaccgcag gcgcgttgtt tcagctgccc atctttcccc
     3181 acaccagcct cgaagaactg cagaatctgg attgcgtgat catggccgcc gacgccggaa
     3241 ccagtgaggg ctgcgtgccc atccgggcga ttcggagtat tcctgcgcga acggtgctgg
     3301 cgatcggcag cgagagtcgg ggcctgtctg aaccggttct ggacgccgcc accgtgcgat
     3361 ttaccatccc gttgaaaccg ggggtcgaat ctctcaatgc cgcggcggcg acggccatcg
     3421 cactgtttca attgtcggga ttgccggttg agggcttgaa ggcctgacgg tcgccacctg
     3481 actccgtttc gccgcttccc gtttcgtctt cctaatccac agccccacag cgcattcaac
     3541 cgtcaggtgt catttcaatc ggactgcagg gagtttcccg tggtttgctg cctcgcgcct
     3601 ccttcctttt tctctgctgt ttcatttacg atgccttgag atctgtgacc ttgaggtgga
     3661 gcgaataggg atgaaggcga agacgacatt tcattgccag gcctgcggcc atcaggcgct
     3721 gcgatggctt gggcgctgcc ccgattgtgg agggtggaac acgctcaagg aagagcgatt
     3781 gccggctgcc cccaaaggcc gccagggtat gcccaaaaca gccatggccg tggccacgcc
     3841 gatctccgat atcgaaatcg tcggcgaacc caggcacagc acgggcatgg gggagttcga
     3901 ccgtgtgttg ggcggtggtg tggtgccagg ctcggtgatg ctgatcgggg gcgatcccgg
     3961 catcggcaag accacgttac tcttgcaggc actgccgctg cttgcggcgc cgggcgagca
     4021 ggttctgtac gtctccggcg aggagtctcc ccggcagatc aagatgcggg ggcaacgtct
     4081 gggcatcgat ggcaaacatc tgctgattct cggggagacc agtctcgaac agatcttaaa
     4141 ggccatccag gaaatccagc cggcggccgt ggtggtggat tcgattcaga cggtctacac
     4201 cgagcagctc acctcggcgc cgggcagcat cagccaggtg caggaagtgg ccgggcagct
     4261 gatgtggttc gccaagcgca gcaacgtgcc ggtgttcatc atcgggcatg tcacgaagga
     4321 aggcgccatc gccggaccgc gcttgctgga acacatcgtc gatacggtac tgtatttcga
     4381 aggggataag agccacagtt tccggattct gcgagcggta aaaaatcgct tcggatcgac
     4441 gaacgagatc ggcgtctttg aaatgaagga tggcggcttg gaggaagtca gtaacccctc
     4501 cgaattgttt ctcgccgagc ggccgcaacg cagcaccggc tcggtggtgg tgtccagcct
     4561 ggaagggacg cggccgatcc tggtggaact gcaggcgttg gtctcgggga ccaattatcc
     4621 catgcccaag cgtatggcca atggtgtgga gccgaatcgc ctgtcgttgc tgttggccgt
     4681 gatggagaaa cgattgggca tgcacctctc agggcaggac gtgtatgtga atgtggtcgg
     4741 cgggatccac atcgacgaac cggccattga tctgggcatt gtcgccgcgg tcacgtcgag
     4801 tttgcgggag agtccgatcg attttacgac gctcgtcatg ggcgaagtcg gactgggtgg
     4861 tgaagtccgg gcgatcagcc aggccgagct gcggattcgc gaagccgcca aaatgggatt
     4921 caaacgctgt cttttaccgg agcgaaacgt cgccaagatg gaacctgtcg aaggcattga
     4981 attgatcggc attcatgaag tcggagatgc gttggatgca gtactggcgt gaagcaaaag
     5041 gtgaaacggt agaggtgaca cgcaagactg cctcgctcgg tacggttgtc gttctgttgt
     5101 gtctctcggt actctccggc tgtgcgctct tcggtccggc tgagaccatc cggctcaaaa
     5161 aggccacggc ggagcagttg acggcactcc ttcaggaaca ggcaggcgag gttcgtacga
     5221 tcaaagggct cttcagcgca aagattaccg gcggcatcct gccgatcggc cagcgcgtgc
     5281 aaggcacggt gttctatcaa cgtcccaacg ccatccggtt gcgcggcttc tctgcggtgg
     5341 gcagcgaaat tttcgagttt gtgcagatcg aagatcaatt caagctccgg ttaccgacca
     5401 tgggacgtga ggtgagcggc cgtccctctg aggcggatca actcggccaa ctgatgcgcc
     5461 cctttcaact gagtgtctgg gcgatgagcg ggatcatcgg gacccaggtg gtcgggcctg
     5521 acgagtcggt acgattgacg gaagacggca gccggtatcg actcgatgtg gtgaccgcgc
     5581 cgggcctgaa tggcacggct ccgttcgtgg ccaggcggat ctggttcgat cgtcggaatt
     5641 tgctggtcgt gcaggaagag cgcctgtcgc cgtccggtga cgtcgaagcc acgatgcagt
     5701 tcgacgacta ccgctcggtg ggcggtgtgg tcgaagcggt atcggcggtg aacggccagg
     5761 cgcctacagc cgataagcgg atcatgcgcc ccttcgccat ccggatgacc gacggacagg
     5821 gctcgggcag tttgcaggtg acgtttcacg aattggtgcc gaacgaaccg atcaagccca
     5881 gcgagcttgg gcgggtatga aggcgaaacc ggcccggcat ttgctggcga tcgatacggc
     5941 cacggcctgg caaagtgtcg cgctgcttcg cgatgatcag gtgctggccc tgatcgaaca
     6001 ggatgccgaa ggatcgcacg cgcgctcgtt gatgggcgcc atcgatcgtc tgttgcgcgg
     6061 ggccggactt tcgttgaagg atcttcaagc attggcggtg tccatcggcc ccggttcgtt
     6121 caccggcctg cgtgtggggc ttgccaccat gttgggattt cgtgccgtgt tgggcactcc
     6181 cattgtgacg gtgccgacct tggaagcgat ggcctggaat ctgcgcgatg tgaagggtgt
     6241 attagtgccg gtcctgaaga gtcggcacaa tgaagtgtat tgggccgcct acgaatggtt
     6301 gcctggaacg ggtttgcgaa ctcacatcgc cgagcaggtc ggtcctcctg cttcggtggc
     6361 ccgggcgtta caaggggcca aagcctgtac gttgttcggc gacggctggc aagcctatga
     6421 aaaggacatc cgggaggccg ttgaagcggc gggcgggcag gtgcgagaag tcaggccgga
     6481 gcaacaacgc ccttcagccg tgagtgtcgg tctcgccggg cgacagcgtc tgggggcagg
     6541 gcaggttgcg ggaccggagc tggttccctg ctatgttcag cgcacggaag cggaagtgaa
     6601 attcgacgag cagcaggggg tgtcggctct cgaacggcgc cggcagcggg tcgccagcaa
     6661 attagcgcag ggccgcggga aacgcggtcg aaccgacgcc gatccgcagc cgcggaagta
     6721 ggcaatggca ttgggaattg agtcggatat ggcgtcagat tcggtgctga ttgaaccggc
     6781 gaccgctgag gttctcgacg aggtattcgc catcgaacag gcttgcttct ctgccccctg
     6841 gacgcgcaaa atgttggcag ccgagttgtc cggtaatcaa tttgcgcagt ttctcatcgc
     6901 gaagtgtcct gacccggggt caggggcctt cgtggtcgtc gggtattttt gcttttggat
     6961 cgtgttcgag gaagtgcggt tgatgaacct ggctgtgctc gccccgtttc gacgacaggg
     7021 tgtggcgagg cggctggtct gtgcggcatt gcgggccggg ttggagcgtg gtgccagccg
     7081 ggcgatgctg gaggtgcggg cctcgaatca ggaggcgttg accctctacc atcggttggg
     7141 atttcgtcag accgccacgc ggacgcggta ttatgtgaac cctgacgaag atgcggtgct
     7201 gatggaaatg gcgccgctcc agttaggtcc cttgtgcgaa cggtcgtagg gcgattccgg
     7261 gagccgggca acaggacagt catccacgaa cgtatgtagt caaccaggag gtgcgacatg
     7321 cagacggaga cggcgatcac tgaacggtta cggcggtcca atacagaatt ccgtgcgctt
     7381 gaagaatctc accaccgcct cgatgccgag ttagccgatt tacaaaagcg gcatgtgctc
     7441 acgccggcgg aagaagtgct gaagaagcag ttgcaaaaag agaagctggc caccaaagac
     7501 aaaatagccg aattcattcg atcgtcgcgt tagcgaagat ctccggcttt ccgtcccggt
     7561 cgcatcccgc cgctgctcac atcgagccgg tgtgagagcc gaaggcgcgg aaggccgggt
     7621 cgttgattca gacaggcaat gctcgcacca ctggccgccc atattcagtc gctcaagaag
     7681 cggctgctga tcatcgtggt gaccttggcg gtcgcgttcg ccacggcgtt tgcctactcc
     7741 tccgagatgg tcgcctggct caatcgcccg tttcccaatc aactggtgtt ttacgggccg
     7801 acggaagcgc tgtttgcgtc catcaaagtg tcgtttctgg cgggcatcat cctcagcctg
     7861 cccatggtgt tttaccagtg ctggaagttt atcgaaccgg cgttgctccc caaggagcaa
     7921 cgctgggcga ttcccttgtt cctgctggcg gcgtttctgt ttgcgctcgg cctggtcttt
     7981 tgtaacctcg tcattctgcc gctcgtcatc gacttcttcg tcagcttcgg tatggatcgc
     8041 gacatcaccc ccgcgctcgg tgtagggacc tatatcgact tcaacgtcaa atttctcctg
     8101 atcttcggct gcgcctttga actgccgctg gtgctgactc tgttgtcacg ggtgggggtc
     8161 gtctcggccg cggtgctggc gcactatcgc aaacatgcca tcatggcggc gctgatcatt
     8221 tcggccatcg tgaccccgga cgcgacgctg tttaccatgt tgctgatggc cgtcccgctc
     8281 atggtactct atgagatcgg gatcatcggg gccaagatct tcgggcgggc cgcagggccg
     8341 gctcccgaca tgaatttgcc gctcgatcct gatataccca tcggcacggc cggtcatcgc
     8401 gtccggtaat ccggcgccca cacttttgga aggatgatca ttctatgctg cgtcacatgc
     8461 tggtgttgac ggcgtttaca ctcggcggtc tgcaggggtt cgcgcatgcg gcatctttcc
     8521 ctggcgggag cgagggcggt gctccgttga atctggagca ggggcatccg gggcaggaac
     8581 cgggcgcctc cgctgatatc ggcaatacga gtattcaggc cctcacggcc ggctccggag
     8641 gagtgattta tgccggttcc ttcggcatgg gcatttttcg cagcgccgat cgtggcgggt
     8701 cctggacgtc ggccaaccag ggattgaccg atcccttcgt gctctgtctc accaccggta
     8761 aggacggcgc gatctacgcg ggtacgtttc gcggcggcgt gttccggtcg cgtgacgggg
     8821 gcaagatctg gcagccggtc aacaagggcc tcaagcgtct ggagatcaaa gccttgatga
     8881 tggatgccaa ggggctctat gccggaaccg gggacggcgt ctatctgttg aatgccgcgc
     8941 aggatcaatg gaaggtcgtg tccaccggtt tggatgagat tctggtgcac gcgctggcca
     9001 ggaccgacga tgggaccctc tacgcaggca cctccgggaa gggcgtgcat agttataagg
     9061 agcggtcgac cggatggacg cggttgcggc aagggctgcg cgaccacgag ggattggtcg
     9121 aaaattttat ccgggtgctg gccgtggata aggaacaggg tgtctatgcc ggcacgttcg
     9181 acggcggtgt cttccgcagc ggcgatgcgg gcaaaacctg gtcgccgatc agccgggcct
     9241 tgccgaacga ttcgatccgc ggtttgatca gcaatgagcg cggcgtctat gtcgccacgg
     9301 gccggggaat cttcaagacc gtcgacaagg ggcggcaatg ggtccccttg aacaaggggc
     9361 tcagcaatct gtcggtgcaa gtgttgatcg aaggtgaaaa agggggcttc tacgccggca
     9421 ccagttcggg ggtctttcgc agcgatgacg atggccttac ctggacggcg gtcagccagg
     9481 gattggaagg ggagagtgtt gcgcccttca agtttaactg agtaggaaag gatacgagat
     9541 gacggcacaa gcgggttcac cggtagcgac catttctgtc acatccaaga acgagccatg
     9601 gggcgagatc gtgctgcgtt tgtttcccga cgtggcgccc ggtcatgtga agaacttcgt
     9661 agatctggcg aagaagggct tctacaacgg cacgacgttt caccgggtga tccccgggtt
     9721 catgattcag ggcggcgatc ccaatagcaa gaatccggac cgcgcatcgc atggaatggg
     9781 tggtccaggg cacaacgtga aggcggagtt caacagcaag ccgcacaagc gggggaccct
     9841 gtccatggcg cgcgccaacg atccggacag tgcgggctcg cagtttttta tctgcgtcaa
     9901 cgacgccaac tttctggact ggcagtatac ggtcttcggt gaagtgcaaa gcggactgga
     9961 agtggtcgac aaagtggtcg gctccaagcg cgacgggcgg gataacccgc tggagcgcgt
    10021 cgaaatgacc gtcactatta ccgagtgatg gccgtgaaac gtaaaaagta acacgtacga
    10081 cgtgaagagg gaaaactcaa agaaagaggg gcgggtcggc cgacgtttcg cgtttcacgt
    10141 gtcaaggatg actgtgtttg ccgcagcggc cttggccgct gcggggtgcg ccattcccca
    10201 ggtcccctcg cggaccgtct acgaagatcc cgtcaatttt gtccgcttgg agttggatgc
    10261 caatgtgctg ccggagtggc cgcctggtca tttcacgcat ccggcccagt tctctcacga
    10321 ccaggtgcgg cgggtgctca tgggccttac ggtgcaggag caccaggctt cgatccagcg
    10381 ctggatttca ggggattcga tccggttgcc gatgtttcgg gatggggaga tcgcgattct
    10441 ggtcccgcag ttggtcgaag cgttacggtt ggcgcgggag aatgagcggg tcacctatta
    10501 cctgagtcag ccgcagacgt cggtcaagcg gatcatcacg tcaggcgggc tctacgtgag
    10561 ggggactgaa ctgcacttta ttttgggaaa ttggcaaacc gtgtacggta ttccggccta
    10621 cggcatgatt tacgatcgcc gctaccccat gaatcccatc gtctccaaag gcttcgacct
    10681 gtttttcgat ctcgaccagg ccatggtgat tcagagtacc agcatctggg actggctcct
    10741 cgccaactcc aaagacgaac tggtgatcga ccttgccaga gtcttccccg gccaaccggt
    10801 ctagcccgga ctattcatga ccgcccgctc cgtcgtccga tcctctcgcc cggcggggct
    10861 tttctcgctc ggcctcctcg acggactgcc agtacgcctg cgtcggcctc acgtgcgaga
    10921 agccccggcg gacttcggtc tcacacgcct cgcgaaggca ttcatgaata atccgggcta
    10981 ggagcctgaa acacgcgtcc atcgtgaatc gggaagaaaa gtcaccactc atcacgcttc
    11041 acgaacgacg agcatggctc gggggaacgg ccgctaattg gctggtgtgc ggggaccttc
    11101 caaacggctg acggtgtcgg gcgcgggctg agtcgcgcgg ctcagcagga gaatgcgccc
    11161 ggtgtcgtcg ccctgagacg tgtaggccag tgtcacttcc gcgatcttgt agtcgaattg
    11221 ggtttctgcc aaccgggtct gagccgcggt caccgccact tccgattgcg tcacttccac
    11281 caccgtgccg agtcccagct tgtagcgttg tttcgaaagc tgcaacgctt cttgcgcagt
    11341 tttgacctgt tcttcggcca gggtgatttg ttgggcgaag gtcagggtgt cgagataggc
    11401 gttggtcacc tgttgcgtca gcgcctgttc gatactgctg gttgcggcgg aggcggcctg
    11461 acgattggcg ttcgcttcca cgacttgatt ctcgatcaga aatccggtga acaggggcat
    11521 cgacaccatg gcgccggcca tccaccagcc gccggtctgc tggttctgtc gggcatcgaa
    11581 gggatcgaag gttccgccgc tggcgatcgc cgagaccgtc ggcaggtact ggcgtttggt
    11641 cgcggtgagt ctcgcatcgg ctgacgcggt ctgctccttc gcgcgtttca cttccggatg
    11701 cgacaggctt tcgttgatca ggctttccag cgtcttctgc ggacgcacct ccacggaaat
    11761 gtcttcgagg acgtagtcgt cggctccggc aatgcccatg gtccggttca agtcggcata
    11821 gctggctttc aaatcgttgc ggctgcgcac cagaagcgat tgcgcattga ccagctccac
    11881 atgcgccaga tcgaagtcga gcttcgattt cagttgctgg cggtagagag tttcgatctg
    11941 cccggcgatg atcccgcgct cccggacggt ttcttcggca atctggacca gccgccgccg
    12001 cttgagacta ttcagatagg cgcgttgcac atacagcagc accagggcac gacgggcgct
    12061 gacatcctgt ccctgcgcgc gttcggccag tttgctcgac tccaccagat tgctcgtgta
    12121 gccgaagtcg tagatgcgtt gattggcgat cacgccgccg gtgaaggtgg tctggttttc
    12181 tcgaagtaat ccaccgccga ccaggaaacg cgggttgctc accccggcgc cggccaccgt
    12241 gttggcgttc gcatagacct gcgggtagta gagggagcgg gcctgttcgg tacgcgcctc
    12301 cgaggctttc aagttcgccg cgccttcgag cagcatgggg tggtttttca ccgcaatatc
    12361 gacggcgcgc tgtacgccca gaaaactgcc gtggggcagc gtcaggggct gctctgcgtc
    12421 cgcggcgcgc gaagcggagg ccaacagcgc ggtcaatgcc aacgtcgtca gcgtcaggag
    12481 ggtggtatgg ggcaacaagc gtctcatggg cgttctccct gtttctgcga ggtcccgttc
    12541 ggtgcggagg tcggcggcag gggagtgcca ccaggaacgc gacgctcgaa tttctgttgg
    12601 gacagtttgg cgtcaggcag attgaacggc gagccttgaa ccggcatacc gtccagcaga
    12661 cggcgtttcc cgaccgcgac cacttcttcg tggccctgca agccggaggt gatttcgatc
    12721 cagcggccgt cgctgatgcc ggtctgtacc gccaccgact tggcctttcc ctcttcgaca
    12781 atgaagaccg attttccttt gggtgtgctg atgaccgcct gtggcggaat cactagggcc
    12841 tgaggaattt cccgcaaccc gagcgccact tcggcaaagg tgccgggccg gagcgcatgg
    12901 tctgtgttgg gcaaatcgat ctcgacgagc aggcttcgcg tcgcaggatc aagcgccagg
    12961 gtggagcggg tgatgacgcc gctgaacgac cggttgggca gctccgtaac cttcacggtc
    13021 gcgctggtct tgcctggaac gacccagggg ctgtcgtctt gcgggacgtt cgcatagacg
    13081 cgaaccgtgt tgaggtccat gatcgtaaac agcggaatcg cgctggtggc tgaggaggtg
    13141 gcgatctgaa tcaatgagcc gggatcggcg aagcgcgcgg tgacgatgcc gtcgtagggt
    13201 gcgcggactt tggtgtagtc ccgcatgacg acccgctgct ccatcaggtg tcgggcgcct
    13261 tgataagccg cttcggccac gtccacgtcc tgtttggcga tcacgtccgg cgattccttc
    13321 cagaccttgg cgagccgttc gtaggtcaat tttttgattt tgtaatcgga caccgcctgc
    13381 tggtactgct cttccacctc gggcgcatcg atcacggcga ccacttcgtt tttcttcacc
    13441 gcgtcgccct tgtccgggcc gatccatttc agatagccgg cgactttggc gtagagcgtg
    13501 gtttgataaa gcggcgacac attggccggt agcttaaccg tataaaccag gtctcgtcgg
    13561 gtcggcttgg tgacctgcac atcgaccggt gtggagtcca gttccgaggg agtcgggccg
    13621 gccggcggtt ccgattgcgc cgtcgcagcc ggtcttgctg gagccgaggg ggtagccggt
    13681 tcgtctccct gccaatagac ataaccgccg atcgccagga tgagcagtac ggccgttcca
    13741 atccagagtg gcgtacggtt gggacgtttg acgagcgggg gcgggactac ctgttcggtc
    13801 atcaaatttc ctccagagtc gcgggcgcgg tcgaagacac agggggacgt tcccggcgaa
    13861 tcaacacgag tgagtgcatg atggggacca ccaacaacgt catgatcgtg ctgaccgcca
    13921 acccgccgac caccgcgcgg gccaggggca ccatcgtttc attgccctcg ccgaagccga
    13981 gggccagcgg cagcaggccg agaatcgtcg caagggccgt catcaggatc gggcgaatgc
    14041 gtcgccgtcc cgcttcgagc acggcatgtt cggccgtggc tccatggcgg accatgcgat
    14101 tggcaaaatc aaccagcaga atgctgttcg acaccacgat gcccatcatc atcagcgtcc
    14161 cgatcatcga ctcgacgttc acggaggtgt tggtgagatg caatgtcagc acggtgccga
    14221 tccagcccag cggtacggcg accagaataa tgaggggatc gatgaacgac cggaacagcg
    14281 cgaccatcac caggtagatc agcacgacgg cgaggggcag ggccagcgcg aagttctgga
    14341 ttgcgccgcg catattggcc acttcgccgc gcagttgcac cgtcacgtcc ttgggcaggg
    14401 gcacgccggc cagcgcttgt tcgatgtcct tggctacatg gccgagatcg tttccgaccg
    14461 gcgtcaccag cacgttgatc aatcgcgaga ggtcatagtg gtcgaccgaa tcggggccgg
    14521 ttttgaagtt gagcgtcgcc acatcccgca acatcaccgg cggtccgcgc cggtcgtctc
    14581 ccgaggcgta atatccgctc gccatgtcca tttgccgtcc ggcaaacggc gtgttctgga
    14641 gcgccaacgt ggagccctgc ataccgccgc ccgtgaggct ggcagtcggc agcagcggtg
    14701 tgcgggcgcc gatgattgga atgttgagca ggtcttccgt cgtagtgagc gattgttccg
    14761 ggtactgcgc cagcagaaaa tagccgttgg cggtcttggg atccagccag tagttggggg
    14821 agagggccag gttggagctg atgccggtga tgacatcgac gaccacgcga ttctggttga
    14881 tgccgtagta cgcggctttg gtgcgatcca catcgatatg cagctcggga taactcttgc
    14941 cctgcttgat ccgcacatcg accgtgttgg gcacctgcgc gatccgctgc tggatctgct
    15001 cggcgaccgg ccggataatc tcaaggctgg ggcccttgac ctggatgtcg atcggtgctt
    15061 tgaggccgaa gttcaacaca tcgctgatga tgccaccggt ttggaaggcg atttcgacac
    15121 cgggcagtga agtcttgagc ttctttcgaa ggtctgcgat gtagtcttcc gtgtgcccct
    15181 gatgtcccgt caccaggttg accagcacga aggccgtatg gttcccggtg ttcggggcga
    15241 agcgcgccgc atcgccgaag taaagacccg tgttcgagat cacttcttcc agatcgccct
    15301 tgggaatcgt atcgtgcacg agttgctcga tttgacccac gatggccgtg gtcttttcga
    15361 tccgtgatcc ttccggcgcc atcaccagca tggtgaaatt gcccgcatcg accttgggga
    15421 agaactcggt gtggagacgg ggcagcagga agaccgcggt cgcaaccagg gcgagtgcag
    15481 ccaggccgat tacgacgggc ttgaaccgca caccggcggt gagcagcggt tcgtagatcg
    15541 caagcacata ccggaaccac ccctcgtcgg ccgatgcctc atgttcggaa gacttggcat
    15601 gccccgcttg gtagagccag gccaacacga ccggcgtcac cgacatggag acgacatagt
    15661 cggccaacat ggcgaaggcg acggtcatcg ccagcggcac gaagagatac ttcacgatgc
    15721 cggtaaaaaa catgatcggc aggtagacga tgaggatgca gatcgtggcc accaggatcg
    15781 gcagcgtcag ctccatggcg ctgtcctcgg ctgccgaacg tccgtctttg cccatttcca
    15841 aatgccgatg caggttctcc tgcaccacga tgttgttgtc cagcagggtg ccgatgacca
    15901 gggcgagtcc gcccagcgtc atcaagttca cgctttggcc ggtcaggtag agcgcgacta
    15961 gcgcggcggt cagagagagg ggaatggcca gtccgaccac gagcgctgcc ttcacgctgg
    16021 ccaggaaaat gaaaatcatg aggccggcca gtccggcacc gagaagtccc tccttctgca
    16081 agttggcgat ggcctggcgg atgtaaagcg actggtcgta gagaaacttg acggtcgtgc
    16141 cggctggaat ctccgtgagc ttcgaaagtt tcgcgcgaat gccgtcggtg acttcaagcg
    16201 tgttggcgcc ttcctgcctg gtgatcggaa tataggtcgc ttcgcggccg ttcacccgca
    16261 cgatcgaggt ctgcaccgcg gcgctgtcgg cggcggtgcc gatgtcgcgg atcaacaccg
    16321 gcgtgccgtt cacgatcttg atggggatgt cgttgatctt gtccaccacg tccacgagac
    16381 tgttggagta gacgtagtag tcgaggtcac cgatcttgat gtcgccggcg ggaataatgg
    16441 cgctctgcgc attcagcgca ttcaccacct cggagggaga gacgccgcgt gcgagcagcc
    16501 gttgctggtc gaggaacacg gtgatctgtc ggaccttacc gcccaatggt gggccgaagg
    16561 aaatgcccgg gatggtggcg atctgctggc gcacgttgaa gtaggctagg tcgcggattt
    16621 ccttggggcc gagcgtgtcg ctggagatgg agaggtagcc caccggcaga ctcgacacac
    16681 cgaacttata taccgagggc ggatagacgc cgggcggcag caggcggatg atgctgtagg
    16741 ccaggctggt cagttctgac tgggccgaat cgatcccgta gtccggctga aagaagactt
    16801 tgatgtaact catccccgag agcgattggg attcgatgtg ctcgacgtaa ctcgcctcga
    16861 cgaagcgctg ctccatcttg agcgtgatcg ctccctccat ttcgctgggg ctcaagccgc
    16921 gatagaaggt ggtgacgaga atcgacggca gcttgatctc gggaaagatg tccacgcgca
    16981 ttttctgata ggacacggcg cccaggatca gcgcgatcat gcagatcgca aagaccgcgt
    17041 agggattttt gagggcggcg cgcgttagca tgaggtagcc atccgtcttg tacagttgtg
    17101 ttccagccag aatttccaga ggtgcaggga gaggctcacc agatcatcga attgtcccgg
    17161 cttgaccacg tagccgttgg ctccggcctg gtagcaggct cgcacatcca tggcgtcgtc
    17221 ggagctggtg agaatcacca ctggaatggg cgccaatcgc gcatcctgct tcaggcgttt
    17281 cagcacatcc accccgcgtt ctccctgtaa tttgaggtcc agcaggatca agcccggttc
    17341 atcgctgggc acgtgatcgt tgagataggt cattgccgca cggctgccat cggtgatctc
    17401 caagcgtccc ttgtatcccg actgcgtcag ggcctggcga aagagttcac attcgccggg
    17461 gctgtcgtcg atgagcagga tggatgaggg catcgtgtat agatccagtg acttctgacg
    17521 cagcgtagag cagggactgt gccggagggg ctttctgcgc agtcatgcta gaaagtgtgc
    17581 gagaaaacga cggcttagtc ctgcgcgacc gggtctgaag gaggggataa gaccttgtca
    17641 gccgcgcgga ggctggagaa actccctaga cgctagggag tttctccaga gcatcgccct
    17701 gctcgatcag gctgccttcg gagctgtctt tttccactcc atcatcttgg ccgtcaactt
    17761 ggaggatgcc gctgtgtagt cacccttcaa gccgatcgac gcttccgcgc cggtcttatc
    17821 ccccgattgc accttggtga cgaccgtcgc cgcgaccttg tggaaggcct tgtgcagttc
    17881 gaggatctct ttgtaatagg ggtctgtggc ggcgggggta ctggccagac tatacagcca
    17941 cttgccgaat tcgcagcggt tgtccacacc gacttctgcc gggaccagtg ccagcgtgcc
    18001 gtcgatgttc tgccgcagtt tcaccttcca cgcgccatgc gcaccgattg ccttgtcgat
    18061 ttgttcgatg atgttggaca tgcgacctcc cgggaaataa agaaaagggt ttcctgttcc
    18121 tgtatcggag ggaggagggg aaggcttgag ggggaggagc gtggcctggt cgccctcttg
    18181 gtcgcggaac gcgcggccta tgaaggcctt cgttggatac gcacaattag aacgatgccc
    18241 tgaggccagc tcactggaca aaaccgtgat gcctcccagg catttgtcga tctgcctcca
    18301 gggattttcc tagcctcccc ggctggcaga aatgagtagc atggttcagg acgacttggc
    18361 cgtaggtacg ggaggttgtg gctgcgacca cagtgcgtcg gtcattccag gaggaacccg
    18421 atcatgtcag aggatggttc agtctcgatt ccaagccgcg ggtggcgtct cattcaaacc
    18481 agagatttcg ggctgctgtg gtgggggcag accacctccc agatcggaga gggcttaaac
    18541 aaggttgcgc ttctctggtt cgtctatgaa ctgaccggct cggtcatgaa gatgaccatg
    18601 gtcggacttc tccagaccat ccccccgctc ttgttcggtc cgctgatcgg cgtatacctg
    18661 gaccggctgc cgaaaaaagc cgtcatgatc tgggtcgatc tgcttcgcgc agtgctcacc
    18721 gttctcatcc cagcgctata tgccatggac atgctgtcga tcgagggact ctatggactg
    18781 atttttctca cgtcggtggt ctccaccgta ttcggtcccg cgctggtgtc ggccgtgccg
    18841 ttattggttc gaccttcgga gctgatgtcg gcgaatgcgc tcattcaagg cacgaacaat
    18901 atcggcatgt tgctcgggcc ggcgatcagc ggcgtgatga ttgcactcat caacgcggag
    18961 aatgtgttgt tcgtcaactc cgccaccttc cttctttcgg ccctgtgcct gatgccgatt
    19021 cgcttcagca tccctcatgt tgcagcgagg gatgcttcct cctccgtgtg ggacgagctc
    19081 aaggtcggat ttcgtttcgt gttcgggcag caacccatgg tcttgaccct ggtgatcatt
    19141 tcatcgctct acaatctggg tgtgagcgcc ttcgtcttca tcttgccggt ctatgccaag
    19201 gagtttctcc aagtcgggcc cgtgcaactg ggctggttgt ggtccgcact gggtgtgggg
    19261 atgttggcgg cctcgacctg gctggcgtgg aaaaaacaga gtgacatgca ggggcgctta
    19321 cgtatcgtcg tcagcggcat gaccatcggc ggccttgcgg tctgtagttt gagcctgctg
    19381 gagacgcctc tggtcgcggc ggcgatcgtc atcatcgtgg gcggcagtac ggcggtgctc
    19441 aatcccatcg tatgggcggt gcttcaggaa gtgacaccgg aacatttgat cggccgcgtg
    19501 ctcacgacgt ttagtgtggg ctccatggcc tccgccatgg cggggatgac gggattcggg
    19561 tgggtggccg acgcgatcgg tcccgctgcg agcctggtgg ggctggggct tgtgctcctg
    19621 ctcactgcag cagtggcggt gtcatgtgtt cgcagagccc gtcccgttct tgtcgcgcag
    19681 gcatgatgat cctcacagat ggcgaacacc tgactgtctg catggaacga acgcgatgat
    19741 acgtggcaag cgagccctcg tgagcggaag gaccatgcgt gagacatttc tcagcgccac
    19801 ccattcctac tgattccact cctgcggtaa acgtttatac tggcggtatg gcccaagccc
    19861 atcggtggct gctcacacgt gatttcagtc tcatgtggtg gagccaggta ctgtcccagg
    19921 tggctgaagg catcagcaag ctggccttgc tctggttcgt ctatgcggtg accggctcgc
    19981 cgctcaagac gaccgtcatc gggcttctac agacgctccc gccgattctc ttcgggccgt
    20041 tcatcggtgt gatcgtcgat cgcttcccca agaaaaccat cctcatcggg agcgatgtcg
    20101 ccagaggagt actcatcgga ctcatcccct gcctggtctc ggtggaacac tttaccgtcg
    20161 ggtcgctcta tctcctcacc tttctgtatg gcgtcgccac ggcggtgttt gtgcccacgc
    20221 tgtcatcggc tgttcccttc atggtcgctc gccctcaatt cacggcggcg aatgcgctcc
    20281 tccaaagtac gaccagtctc ggtatcatca tcggacccgc ggtcagcgga ctcggtattg
    20341 cgtttgccgg ttctcaggac gtgctctgcc tgaacgccat gacctatttc gcgtcagccg
    20401 catgcctgct accgatccgg ctgcgagtga cagaggctac aacggccgat ggagatggct
    20461 ggagaccatt tatccggcac ctgctcgaag gcatccgcta tgcgtttctg acccaccgca
    20521 ccgtactgat tctgattgtc ctcgcgtcgg tctatacgtt cggcagcggc gcattcacga
    20581 cgttgttccc ggtgttcggt cgccatctgc tcgggctcgg gccggtcgag gtcgggtatc
    20641 tctggtcctg gcttggagtg gggttgctgc tctcttcaat cgggctgatc cggatgagcg
    20701 ggtgggacct ttcgcagcgg ctctcggtaa tcactctctc ctgcgcactc gcggggttgg
    20761 ccttgttggg cctgacgtgg acggatgatt tgcgcctggc tgctgtactc gtgggctgca
    20821 tcggcatcgg cttcggcgcg tggaccccca tcgcctgggg catcattcag gaagtggctc
    20881 cggcgggaat ggtcggccgc gtgatggcgg tctatacggc gagtgccacc gcgacatcca
    20941 tgctcggcat gagtgcgttc ggctgggcgg cggagtcgtt cggatctgtc gccagtctca
    21001 ttgggatcgg cggagtgatg gttattttgg caggcgggag tgcctggttt gggaagagga
    21061 ttggtgtcgc cgtaatcggc aaggcggatg tgtgacgtgc agttgccggc ttgtcactcc
    21121 ctgccttctg tgcgctggcc ctccagcgga tcgctgtgcg ggcctgaaga attttcagaa
    21181 gctctctcag gatctggaag acaagtggtc catctgtgat aattcgggac cggtgccaag
    21241 actggtggag gggaagtcat gaaatcgcca ggaggaaaag ccagggctag caagttttct
    21301 aagcaggtcg gcgccgcgtt gcgccgagcg gccaaggttg cccggaagac cgctcgcatg
    21361 tacggcacac caatttatgt gtgggagaac gggaaggtgg tggcgaagaa accgtgatgc
    21421 ctgtatcgaa cgagatgcca agaccaacca actaacctat ttccacgctc acctaacatt
    21481 atacttcctg cttcgaggct gccgggtatc cggcaagtcc ccttactacc tcatcggcgt
    21541 tccaaacgtt gatcgctcaa ccactgcatc atacgatcaa ggcttgccgg tcttatcgcc
    21601 cggcaaggca ctcctcagtt acctcggact caacaaccac cgccatcagc cgcttctcta
    21661 tggcttgcgt gattccggcg tacacggtgg ctgcagcggc agaccaagcc gggttgaagg
    21721 gttcaccttt aaacgccgct tcggcagcct tcgtttcatc atgggtcagt gaccgttgtc
    21781 gggtactgtc tgagtgtttc gactccgcgg tgtctcgatg gctttcgtct ttcatgatgt
    21841 cctgtcgcat aagatcctcc ctctgtgaga cgaccgttga atggtcgctt gtccgataaa
    21901 tgagcaaact tagtgccagc tcagcacttc tgttctctat gtcacgaagg tttcttcggc
    21961 ggaatgctca acggagaatt cagggctcat gattcatcct accatgaaca tattttcata
    22021 cagttagcgc ggggatgtgg ctcgccatga ccctaggaac ggcaggtttc taccctggtg
    22081 gttcacagga cttccccacc ggagatgccc ttgcgggccg atccgtatga cgaaaattac
    22141 agggatatga cagttcggtc catatgggca ttggcaaagt gctaaggata gtatctcttt
    22201 ggatgcagtg ggggattgat tcagtgggat ggtcagagtc aagggcccgc gagttccgcc
    22261 tgttcggctc gtgccgattg ccgtgtctgg ccctgcccct ccgcctgctg ctgttcggcc
    22321 ctccagcggt agagcagatg gtcagcgata cccaggtccc gggccacgtg tgtgacaggc
    22381 cgtgccgagt ctcggaccaa ccggaccgct tctgccttaa actcgtccgt gtactgccgt
    22441 ctggttttcg tggtcattcc ttcctccgat ctcatcacat tcctccttat acaggtgtct
    22501 gtgaaatcgg gggaaggtca ggtagatggt tctgcttatc cagcgtagga cagacctgca
    22561 ctagataatg gcgagaattt cagcaatacc tcaataagaa tacccgtttt tacaattgaa
    22621 atcacccaca ttgcttcccg agcaagatgg atattgcctt aatatttgga ggtgaagtga
    22681 gcacttggat gagcacttct tcgaccgtca ggcgagagtt cacgctgcgg aaacagactc
    22741 tggtggcaaa ttatgatgag tggtgccgta aatgccttgt tctataacgc ccgtgtcgtg
    22801 gactatcaaa attttttcct gattctcgcg ctgtctagct cttgctcaac ctgctgcctg
    22861 ccgaggagat actagggcga agttgcccgc ttatatctac aatgaaaatg cttgtattcg
    22921 tgtcatggta gggccatttg gccatattgc gattgcgtag taattgattt acaagagccc
    22981 aattggaatt gagtcgcaga caggctaggt gcctgccgcc gagcaatgga ggtacaaaat
    23041 aaagcagcta ggcgttgatg aaatgaagat cgagtggcgg aaaatatcgt gcgttttcct
    23101 aatccgggag ggattatgaa aatatggact gttttgtttt ttctcacctt gctttacgct
    23161 ccagcgtatt cgcagatagg ctcgccatgc ggagttccag tactattgca gcccactccg
    23221 cttaatgccc aggctaccct aaaccctttt aatggctcgc cgataatcct cctcaatccc
    23281 cagtttgtca tgatggctgg ccaggcaggt gttcgctttg cgttggccca tgaatgcgcg
    23341 catcaccagc taaaccacgt agccatctcc cttcaggttc tccagagtaa cccatatgta
    23401 atgccatgga taactccaca aatagagctt gctgcagatt gtcaagcagg agcattccta
    23461 ggctccgtag gtgatatgca agcgattcag gccggattgc aatttgtggg aggggcaggg
    23521 ccatttccga gtggtcctaa ttacccaacg gggtttcaaa gaatgcaagc catcagaacg
    23581 ggggcgcaga tgggacattg ctagacgtat agttgaggtc attacggggt aaacaccatg
    23641 tccgtgagga aggcgcctcc atggaattcg gggatcagtc aatgagaccg agtctattgt
    23701 ctgcaatttt cgcatttttc attctgactg gcatctgtgc atttacacaa tctggctttg
    23761 ctgatgagca gccggtctct gcctctcatc aaccttttca ggattggttg attgagaaga
    23821 ttgatgcgga tccatcagag tgggatcggc ttagggatct aattctgcct ataagtaagc
    23881 gccttgcgta tgatgctcac caaaaagaag tcgttatcct tccatatgac atacctgatg
    23941 tagttttgag accgatgtcg gccgatgcta gggaagatcc tcagcttagg ccgtttatcg
    24001 gcgctgcggg tgagcctccg cgactccgtc catttttgtc tagtggtggc atgtggaatt
    24061 gggtgactct taaccctttc ctgcctggac attacgatgt cacattaggg gtgctcaatg
    24121 aagaggggat gccttggtcc ttcagttctg aagcggctga ggttgtggca gatgcgagtc
    24181 gagatccaga tctcttcgaa tggaactgtc cggctgctca tgcgcaaaat cctatagcat
    24241 ccacggtgac atcaagattt gatttcacaa gagggaaaga gggctttttc acatggatta
    24301 agggacgagt gaatgaaata cgggaagctc aagtagctgg cgatgacaga ttagcgcttt
    24361 atcatttggg aagggtgttg cattcgatac aagatttagc ctcacatcgt ggaatggaca
    24421 atgctgagca ttcctggttg gatcaccagg gagaaggccc ggatgaggat accttgataa
    24481 atatacccct agccaaaacg ctaaccagag acttcttgga acatttatca gagatctcgc
    24541 catcaataaa ttatgcactt ctcaggaacc cgagaaatgc cctatcaact tggggatata
    24601 aggaagtaaa ggatcgttta gggttttcac gacagctaac taagaaagaa tacgacgctt
    24661 ttaagaagct tggtagccag ttgccagcga gccttaatgg attgactggg gtagaatttg
    24721 aggtggcagt gaaacaacta aagaaccgat ggttcactcc agctgactgg cgaaaaatga
    24781 tttcggagat caaggagcac tcactcaagc tccccttgtc tgagtcagtg ctaaccacat
    24841 gccattgaat tcggagaccc tatgactaat tttttatggt gcactgttac attgttgctg
    24901 cttgtatcta gtgcccagaa ggtcatcgcg gaggatgagg atcctctgag gcgtgccctt
    24961 cgagcaacgg ttcaaatagt gatcagggac agtgccaaca ctgtccgagg tacaggaagt
    25021 ggcgtgctgg tatctcatga cggatacata cttacggcga agcatgttat tgaggactat
    25081 tacaaatcac cggaaagtac ttcgatactt gtgagaatat tagatggcaa cctcacgtta
    25141 cggccggagc aaattgctga ggctgtatcg cgtgatccaa atgtcgacat ggcattgata
    25201 cgcgtaacag gtgaaaagtt tcccttcctg aggattggaa actcctcaat ggtcaaaatt
    25261 gatgaacaac tggttgcttt tgggtttgag cgaagcggcg gcaaatggct gacaaagtca
    25321 ggtcgccgta atggacttaa ttgggaagta gatattccgc tgttcccagg ctctagtggg
    25381 ggaccggttg ttaagagtgg ttgcgatatg cttgttggta tagttgtggg aggtttagaa
    25441 gatcctatca ctaaggcctt aattcctggt cggtcgcaga tacttccctc gcaatttggt
    25501 gcttttgttc tcggatacgg tgcctcaaca cagtcagcga agttgtgcga tagtatggtg
    25561 gaatcacctc tgcatagtcc tactcctaac cgtccaccca cttctccctg caaggaaatt
    25621 acggtgtctg aaacggtaaa cggagttacc acatgggcca agaagtgcct ataggaatgt
    25681 ggtaagaacc tttcctgtaa taaggaggga aaatgttgat ctcaaggcta gtcatcgttg
    25741 gcctatgtat ttgtttgctg cttacagctg cggtttcact tgcagatcag acagatgaag
    25801 ctagagccgt tgccatgact attctgcaga agctggagca gaagaagaat tcagaagtgt
    25861 gggaatcaca tgtgtctaac tggtttaagg aaaggaccac caagccagcg tttcttgcga
    25921 atatgaccct gatccaatct caactgggag ggatagggtc gcaccgagaa cttgtgcagc
    25981 agaatcttgc gaatagcagc ccgcaggttg gttacagagg cgaagtgtat tcgttcatgt
    26041 ttgcgactac gtttccttct gccaatgttt acgaaatgat cgtgatgatc aaagaggata
    26101 gcacctacag actttccggt attcaataca cgcctaatcc aaacgtgagt tccccataag
    26161 acagagacag tcaaaacgga gaacattctg gaaggtgacg ttggccccgt tcttagacgg
    26221 tgcttaacca gagcagacga atttgggggt gatctgttta gtctgacatt gtgcgataaa
    26281 ctcattctgg ttccggtgcc ctcgcagtgt gtgtattatt ccatctcgac gcaaggccca
    26341 tcctccgttg cctgcccctc cccccatatt gactcccccc tgaatccccg ctagcctctg
    26401 ccccgccaga caatcacccg gttaccagga ggttaggcca tgacgctgtt ccgatctgtc
    26461 gcaatgttgg tctcgttggt ccttgtgcct ctgacggtcg gggctgcgcc gccaccggag
    26521 ccgacggtgg aatattcggc ggacagcacg atggaaaccg agggtggtat gacaatgaag
    26581 tcccgcgtct atcacacgcc cacgaaacag cggatggaaa tgggtggggc ggacggcagc
    26641 gtcaccatta tccgcaaaga caagaaggtg gtctggcagg tgatgggcga tatgtatatg
    26701 gagatgccgt tggatcaatc gaacgcgccc gacatgggcg gcttcgacat tcaggaacag
    26761 accgaggtcg gtgaagagac ggtcaacggc ttgaagacca caaaaacgaa aatcatcgcg
    26821 accaagccgg acgggtcgaa attcggcggc ttcttctgga ccaccaaaga gggtgtcacg
    26881 gtgaaaatgg atctgctgtc gaaggacggc gataagaaga tgcgtatggc cagcgagttg
    26941 acgaatgtga aaatcgagaa gcagaaccct gcgctgttcg aagtccccgc aggcgcgacg
    27001 aagaacgata tgggcgccat gatggggcag ggcggcatgc cgaacatgga agagatgatg
    27061 aagaacgccg ggcaggagcg ggcgagggag aaacgctcgt cgaggagcaa cagcgggagc
    27121 aagagccagg agagcgagac gcctgctgcc gaggtgaaca agatgctgaa aggtctcttc
    27181 ggccgatagg ggaggtgcgc gatgcgtacg gtcagaatcc tgagctgtgg cctgctgctg
    27241 cttgccgtga cggtgtcccc ggttcgcgcc gacgatccct gcttggggga tgaggaggag
    27301 aagagcgcca aggcggcggt cgccgctctg acgaaggccg aacaggccgg ccggccggct
    27361 gagctgttcg tgacctatcg atccatcctc ggcaacgact gtctcgatcg ttacgatagg
    27421 accgccttgt cccgcgccaa gagcgccttg ccgaagttgg gccgtgaatt ggccaaggct
    27481 gccgaggtga agggcctctg gtactcgccc gatccggtgc ggggcgatgg gaagacctcg
    27541 gcgtttcgct atttcgaaac catcggggac tatgccgacg cgaatcgtgt gatgatgaaa
    27601 gcgctgcagg cgaaaccgga ggatctcgcg ctcttcacga cggcctgggg tgtggatgaa
    27661 ggtcgttggg tggtgccgga tccgaaaacg gccgagcgtc agccctacgt ctcgccgcag
    27721 gcctatcgtc aggagttgca gaagatagcc tcctccactg ccgatcggct gatgaaagcg
    27781 gaggagcagg atgcgaaggg actctcgggc agcgcggttg aggtggccac ctccgcgatg
    27841 aaatctctcc agaaactgcg aaccgcagcc gggtggatga agttcttgtc ggtcggcgac
    27901 aagacggcgc gggagcgggc cgagcagcgg ggcgatacga tcacggcgcg ttcggattcg
    27961 acgttcaccc aggcgaatgc cgtgatgtat tacgagtttg ccggctcgcc gaagtcgaaa
    28021 gacaaactga cgcaggtgaa gaagaagatg gatgaatcca gtcgtgcgtt ggagaagtcc
    28081 ggtgagaaag tgaagggagc cttcgcggag cagagtcagg cggagcagaa gcaattcgac
    28141 aaaaagaaag cggatctcga aaaagaactc ggattctagc catggcccca ggctttcccc
    28201 gccgttcatt gtcgcttgaa cggagggttc acctcggcta tcccgtcttc ggtacgatgg
    28261 aaatcatgcc gttcgtttca aggatcgcat acttgatttg gtccatccgt tccaaaccat
    28321 gtttctccct ggcggccgcg aggatgtcct gttcatccac gcggacccgg tacatgaggt
    28381 ccttcagcgg acggccttga tcgaccagga tcagcggcag tccatccagg tatcgctcca
    28441 tcgcaggaaa tcgctgcttc atataggaca agacaatatc gagcccgacc agtgtcagga
    28501 tgacgaggaa gccgttcgtg acagagtaat catttccgat catggcattt tgcgtcgcct
    28561 cgctgatgat caagaggagc acaaagtcga agttcgtgat actgccgagg gtacgacgac
    28621 cggcaatgcg aaaaagaatc atgaggaaca gatacaccac gagacctcgt acaatggcgt
    28681 ccatgggtgt ctccttctat ggccatacga actgcgtcac ggaaagtggc tcgccatcat
    28741 cgaccctgac gctgccatgt accgtcccca cggcatcgag ttgaaccgac agcgtggccg
    28801 tgaaccggct gccgtcggat tgaaagacga acactcggcc cccgttgcgc gcctcgactc
    28861 gaatcggcgt cggcgatatg gaggttagct ttgacgcgtc cagatattcc tgatccaacc
    28921 agacacggac caccccatcc cgatccttga gtggttcaac ggtcactcct atgtcgccgc
    28981 tcttctcaag gcgcgcgaac cggtcgtaat gaattgaaag tcctccgtcc gcagtctgct
    29041 gagtcacaga ggcgagcggg tgatgcccga agagcccgag cagcgcggct atgaggcagc
    29101 aggcgatcaa gacttgcccg aaccgttcca ctcgccactg gcgatattgg aatggcaggt
    29161 cctgttctat ttgaatatgc ttgtacgaaa tcgtgatgat cctcccgtct catcatgaat
    29221 gaggtcgccg ggtattgaac ataggagtaa ccctagcgct catgacggct gatcctgctc
    29281 atttgccggt agggtgcagc taacctactc cgccaacgat cttccgcatc acctgatttt
    29341 cccaagggct tctcctagtc cttcaatagg cagaaaacgg ctattctcta ctctactgag
    29401 tatgtcgtag ggagatccta cagacgactc gttaacagga gggtaggaga tgtcgaaatt
    29461 gttcatatat ctgtgcatgg tagtggctgt gctcggaatg acgagtcagg ttggaagggc
    29521 cgacgaaggc tcccccaata gtcaccacca ggagctgcaa ggggtggtgg tggagaaggg
    29581 cggagcgctt gcggtgaagg ttcccaacgg cgcaacgtat cagctcaatg agaaccgcgc
    29641 cgagcgtcat ggtcatgcat tgccgaaagc cggcgacgaa gtcaccgtgg tgatcgacga
    29701 gaacaacatg gtgctggaag tgcatcccaa gggcacggaa ggcaaacatc ggtttgtcac
    29761 cggtgaagtc gtgtcaatcg gcaccatgca gaatgagatc accttaaaga ccgcgaaggg
    29821 cgagcagaag tttccgctgg agaaacaagt gctctcagcc tcgatcaagg atggcgcgcg
    29881 ggtacgggcg gagttgaacg aagccggcca catcatcgat gtgcatccgg aaaagtccgg
    29941 cgcgcattga tgcgagggtg gcagaaatag tgaatgggaa accgggctgc ggagggccgt
    30001 tgcccggttt ttctgtgaaa actgctcagt gggtggcgac actctgctgc tcttcaaacg
    30061 tccccgttcc gcatcccaat cctccgtaca tcatcccgaa gaagccgcaa taggcaccgg
    30121 ctttgaacgc ccaatcggcg cgtgtgacgt tctcgatccg gccgtcatgg atgaggaacg
    30181 atacgtattg tgaaccgacc ggaagcatgg cggggagggg aacaatgacg acatagagaa
    30241 cgagtccgga ccatcgcaag ccatcggtct tatagatcca ttcttccttg ccgccttcaa
    30301 gcgactctac ccgatctggc tcgccccact gtgcgcggag ctcggctacg gtcttctgtg
    30361 tccctttggg ttcgccgttc cgcatgtcga gcacacctct cgttcgatcc attttagggt
    30421 ggtcgctgga ttctgtgcga ctacccagcg tccaggcccc gacaccgacg cacccctgca
    30481 gcagcaaagc cccggccatc cacatcacaa cctgtcgata cctccgcata cagccctttc
    30541 tttctcgata gaacctcggg ggtcgccgtt tcattttccg ccgcagccta ctcagatctc
    30601 gccggcaaca caaggagact cacggatcgg attgtcgtct caccctccca aggtaggata
    30661 tcggtcgcag ggccggcctg agtaccatcc gcccacaggg aggatctgat ggcacctcgt
    30721 cggtacgttc gaacctacta ccggtttccg ctcaactatc cggtcatctt cggcggggca
    30781 ccatttgttg gagaaggtgt tctgacgaac ctgtcgctga ggggttgttc cgtcatgtgc
    30841 gacagagaag tcctctgcgg aagtgacgtg cgcgtgagtg tgctcctcga ccatcagccg
    30901 ccggcgctcc ccatcgacct cggcacgatc aaatgggtga aggggcatca gtttggcgtg
    30961 gagtttgtcc gtcttcccct tgaagcccag caacgtctca atcgcacgct ccggactgaa
    31021 ctcatcgaat ggttgaagag ccgtcagaac agcagtgacc tgccggaaac ttccaatcca
    31081 gacaactgat ctgcgatcgt ttcctctctt ctcgcggtgc cccctagttt ctcgtcaacg
    31141 aaatcacgca ctcttgcccc tccgcagcgg ccttgctctg atggccgctc gtgccctgcc
    31201 cagacaaacg gtcttcgcgt ttccagtctg atcgacccgt tgcttcaagt tatcagaccg
    31261 atgcgccgat gtaagaaatg gactggcggc atagttcgac acgcgtatga acatccttca
    31321 cctcggcaat ccgttctttc tgcaggactt caggcagctc ggccatgatg tcaaatgggc
    31381 ggcctacgat cgcacggccg atttcgtcct gagtccgtac gtggaaagcc tgcatagtct
    31441 ttcggcgcag tttccggcgc gatggtttcc cgacttggtt gtggtggggg acgatagtgg
    31501 gccgccgaag ctgttagggc tggaggcgct cgacgtgccg ttggtgtggt atgcgatcga
    31561 ctcccatctc catgcaagtt ggcatcaaca gtatgcggcg gtgttcgacg tcatttgtgt
    31621 ggcgcagaga gactgggtct cccggtatcg ggtcgatcct gaccggcaaa tcgtctcgtg
    31681 gcagcctctg ttctgtcatg tgcccgacga tggggattcg ggccgcttac gggatacgcc
    31741 cctctcgttc gtcggaacac tcaaccggca gtggaatccg gagcgtgtcg cgttgatcga
    31801 aggcctgcaa cgacggtatc ccattgcggt ggggagcggc gcgtaccgcg aacccttcaa
    31861 tcgttccctg atggtgctga atcaatcggc cgccaatgac gtgaatttcc gtaccttcca
    31921 ggccatggct tgcggggcgc tcctgatcgg cgagcggatc ggaaacggtt tcaacgactt
    31981 gttccaggat cggacccatt gtgccctcta tgacaggggc aatgtcgagc aggtgatcga
    32041 gatcgtcgca cactactgcg gccgtccggc cgagcgggat gcggtggcaa ggcagggata
    32101 tgaggcggtg atggcctccc acaccagttt gcaccgggcg caggccattc ttcaggtggt
    32161 ggcgacggcg ccgcttcaag gccagattcg ggttcgccgt tcgcggcaac tccagattca
    32221 atcgtcgctc gtcctggtat atgaaagcgc cgaacgcacg catctccagg cagccgaacg
    32281 gacgccggag ggacccagga agcagtacct gctcacggtt gctgagcagt atcagtttct
    32341 cgccacgacc attcgcacgc aactcggcct ccaggtcgcc tgctagcccg gactattcat
    32401 gagtgcctat tacgtcgtcc gatcctcccg ccccggcggg gttttctcgt tcggcctcct
    32461 cggcggaatg ccagtacgcc tgcgtcggcc ttacttgcga gaagccccgg cgggcttcgg
    32521 tctcgaacga cttacgacgg cattcatgaa taatcctggc tagaggcgct tgaagcctcc
    32581 cgccacgatc gctactcatc ttgcgcagcg atgcatcgtg acgcgtggca tagaccacgt
    32641 tcacccctgt tttgaggagg gcaacgccgc cgcggcctgc catttccggc cggtcaggcc
    32701 caaccgttat agaggcttcg gcgggctagg tttctccctt ctaactccgg tataatccgc
    32761 aattcattcc gttctaaaag actggccccg gtccagttca cgttcggtca actcagcgcc
    32821 ggctggtgcg ttgtctccca cggcgttcga tacaagaaac gcttcagagt cacgcttcga
    32881 taaggagttt ggaatgactg aaaaagacga aaaaaagcgc gcgttggacc tggccctggc
    32941 ccagattgaa aagcaatatg ggaaaggtgc ggtgatgaag ctcggcgcgg acgaccgtcc
    33001 cgcggatgtc ccggcgatct ccagcggctc gttggggttg gatattgccc tcggtgtggg
    33061 gggcttccct cgcgggcgcg tgatcgaaat cttcggcccg gagtcttccg gcaaaacgac
    33121 gttgacgctg catgccattg ccgaggcaca aaaggccggc ggcgtggcgg cgttcatcga
    33181 tgcggaacat gcgctggatc tgacctatgc caaaaagctc ggcgtgcaga ccgacgatct
    33241 cctcgtctcg cagccggata ccggcgagca ggcgcttgag atcgcggaaa ccctggtgcg
    33301 cagcggcgcg atcgatgtca ttgtggtcga ttccgtggcc gcgttggtgc cgcgagcgga
    33361 aatcgaaggc gagatgggcg attcccacat gggactgcag gcacgactga tgtcgcaggc
    33421 gctccgtaaa ctgacggcag cgatttccaa gtcgcagacc accctgattt tcatcaacca
    33481 gatccgtatg aagatcggcg tgatgttcgg caatcccgaa accacgaccg gcggcaacgc
    33541 gctgaagttt tattcatcgg tgcgtctcga catccgccgc attgaatcga tcaaggacgg
    33601 tcaggatgtg accggcagcc gcgtgcgggt caaggtggtc aagaacaaga tggcgccgcc
    33661 gtttaagcag gcggagttcg acatcatgtt tgccgaaggc atttcgaaat ccggcgagct
    33721 cgtcgatatc ggcgtggaga agcgcgtggt ggaaaaagcc ggggcctggt actcctacaa
    33781 aggtgaacga ttggggcagg ggcgtgaagc cgtccgcgat tttctcaagg cgaatcccgc
    33841 cattgcgaaa gagatcgagg gcaaggtgcg cgatctcgcc ggtctgcctt cccgcggcac
    33901 tgaaaagaag gtcgagggga aagaagccaa ggacgagaag cccgaacgga agccagaggg
    33961 acgccaggac gataaacgcg gtcacagtgc cagagtcacg acctaggtgg tggcagtgcg
    34021 gacagcgcgc agcacagagc ggcgtatgaa accgacgccg gattatctca cgctggcgat
    34081 caagtatctc gcgcggaccg atcggaccgt ggcccaggtt gagcggtatg ttctggaaaa
    34141 aggtgcgagc cgggcggaag agcgcctcgt cgttcgtgaa ctggagcgca gaggttatct
    34201 gaacgatcag gcctatgcga tccgatgggc ggaaacgaga ctgtcgcgac atccgatggg
    34261 ccgtgaacgg ctcaaggcgg aactgctcag ccgtggcttt gaggacagtg tcgcagaacg
    34321 ggcgttgcgg caggcatacc gttcgatttc cgaacaggag ttagcctgcc aggcgctcga
    34381 agggcgtgcg agccggacgc gtccggctca atgggtgcgg tttctgcggc aacgcgggtt
    34441 cgatgacgac acgattcagc aagtcactca agtagatttg gagacggggt tggacgagtt
    34501 atgagccaaa gtgtgaacga tctgcgacga gccttcatcc agtactttga acagcaggga
    34561 catcgggcgg ttccgagcgc tcccttgatt ccccaggcag acccgacgct gctgtttacg
    34621 aacgccggca tgaatcaatt caagcgggtg ttcctgggcg aggagacgcg tgcgtataac
    34681 cgggcggtga ccgtgcagaa gtgcctgcgc gccggcggca agcacaacga tctcgaaaac
    34741 gtggggtaca cgaggcgtca ccacaccttt ttcgaaatgc tcggcaactt ctccttcggc
    34801 gactatttca aggaagacgc cattcggttc ggttgggagt ttctgacctc agtcgtcggg
    34861 ctctcgaaag accggatgtg gatcacgatc ttccgcgagg acgatgaagc cgaccgtctc
    34921 tggagaaaga tcggcgtctc cccgagtcgg attgtacgtt gcggcgagaa ggataatttc
    34981 tggcagatgg cggatacggg cccttgcggt ccctgctctg aactccattt cgatcaaggc
    35041 ccttcggtgc cgggcgacgc gacgccgaac ggagaaggcg accgggtcat cgagatctgg
    35101 aacctggtgt ttatgcagtt caatcgcgac agtgcgggca ccctgaatcc gctgccgaaa
    35161 ccgagcatcg ataccggcat gggcctggaa cggttgacgg cggtggcgca agggcggctc
    35221 agcaactacg acagcgattt atttgcgccc ttgttggcgg cgatcggaag cagggccgga
    35281 gcgcagtacg gtgtgctgga acaagcggat cgttccatgc gcgtcattgc ggatcacttg
    35341 cgggccatca cgttcctcat ggccgacggc gtcttgccgt cgaatgaagg acgtggatat
    35401 gtgctccgcc ggattctgcg acgcgcggct cgccatggcc ggttgctggg tattaccgaa
    35461 ccgtttctgc atgaactgac ggcgacggtc gtggagcaaa tgggcgaggc ctatcacgaa
    35521 ttgcgtccgg cagccggtac ggtggcggag gcgacacggg gcgaagaaga acggtttatc
    35581 gccaccctcg accaggggtt gccgattctc aacgacatgc tgaccaaggt gcgagcctcc
    35641 ggtcaaaaca tgctctcggg taccgaaatt tttaagttgt atgacaccta tggctttccg
    35701 atggacctca ttgcggaagc ctgccgtgag caggacatcc gactcgatga gacgggattt
    35761 gaagcggcca tcgaagagca gcgcacgcgc gccaggaaaa cggggggctt cgagggtgag
    35821 acggcccgcc ccgcattgag tgaagtggcc gctcgtgtcg gcactacggc attcgtcggc
    35881 tacgagcacc tgaattcgga aggtgtcgtg caggcgttgc tcaagggcga tcggctggtg
    35941 aaggacgcgc gtgaaggtga cgacattgaa gtggtgttgg atgtcacgcc gttctacgca
    36001 gaaggcggcg ggcaggcagg cgatcaagga ctcctgaccg gtcccgatgg ccgtgtggag
    36061 attcgtgaaa ccactaggcc ggtgccgacc ttgatcgtgc acaagggcgt agttacatcc
    36121 ggttcgatcc gtgaggggga gcggttgcag ctctccgtga atcgtcgcac ccggcacgat
    36181 gcggcccgca atcatacggc gacgcatttg gtgcatgccg ccctgcggga tctgctgggg
    36241 ccgcatgtga aacagtatgg ttcgctggtg gcgcccaatc ggctgcggtt tgactttgcg
    36301 catttccgcc cattggcctc acgcgacatc gacgagatcg agtcgatcgt caatgagcag
    36361 gtgcgtcagg atcagccggt gcaaacggat gtgatggggg tgcaggaggc cgtggccggc
    36421 ggggcgctgg cgtttttcgg cgacaagtat ggcgatcagg tgcgagtggt tcatatcgac
    36481 accttcagca aggaattgtg cggcggtacg cattgccggc gcacgggcga aatcggcctg
    36541 ttccggatcg tgtctgaatc gggagtggcc gccggtgtgc gccgcatcga atgtctcacc
    36601 ggtagcggcg cgttggattc gctcaagcga ttggaagcgg atgttcgtga gttgtccgac
    36661 ctcctgaaag tggcgccggg ggaagtcgtc gcccgtaccc gcaaactgag cgagcaactg
    36721 aaagagaagg aacgggaact cgcggaagtg aagctcaaga tggcgagtac ctcctcaggc
    36781 gacgcgcaag ctcgcgagat caaaggggtg caggtccacg cgcaacgcac ggacggcctc
    36841 gatgtgaatg gcatgcgggc gctggccgat caattgcgcg acaaactgcg cagcggcgtc
    36901 gtggcgctcg gggcggcgaa tgacggcaaa gtatcgctgc tcgtcgtggt aacgaaggat
    36961 ctggtgggcc gtctgaaagc gggcgaactc attaaggaga tggcaacgga agtgggcggg
    37021 accgggggag gacgtcctga gatggcgcag gccggaggga aaaatcccga gggactcggc
    37081 accgcgttag aaaaagtttt tgggttggtc cagaaggccc tcgaagggta gactgatgaa
    37141 aggccaacgg attctcgcca tcgatcacgg ctccaagcgg atcgggtttg ccctgagtga
    37201 tgagctcggg tggacagccc agccgctgga aacgttttac cggcgaaatc ccgatgccga
    37261 tatccggcat atccaggacc tggttcgtga acacgaagtc gggcaggttt tggtcggcat
    37321 gccgttgcgg ttggatggcg agatcggtcc cgcggccaaa gtcgtcgcgg agttcatcca
    37381 gttactggag ccggcactgt ctgtcccggt gatcacctgg gatgaacgga tgaccacctg
    37441 cgcggcggag gacctgctga ttgcggctga cgtcggccgc cggaagcgga aggggatcgt
    37501 cgaccgtatt gccgcggcga ttctcctgca gagttatctg gcgagtttgg aacagccggc
    37561 caccagccag gcgaacgatc tgaacgcacc cgtggagacc gatccctggg tctttgacga
    37621 accccgagtc gatgatgcag aagaaagcga tcatgggtct ggccctggcg gcagtgatgt
    37681 tcgcggctct cgcgggatat ctggtgctgc gttgggctca aagccccgtc gcgagcggac
    37741 ctcctaaacc tccctcccac attgtcctca ttcctgaagg cagcaccttt cagcaggtcg
    37801 ccgctcttct caaaaacgaa cagttgatcc gcagccggtc ggctttttta ttgctgggca
    37861 agacgcggga catcgatcga aagatccgcc ccggtgagta tgagttggat gccagtatgt
    37921 cgccgcagga cattctgacg aagctgctgg ccggtcgggt ggtgctgcat cccgtcacga
    37981 tcccggaagg gtatagcctg acgcagattg ccgaggtgct ggcggcgcag caggtgacgg
    38041 acacgaaaga gttcacgaag ctggtgcgcg atcgcacgtt tatttcgacc ctcggcatcg
    38101 aggccgattc cctcgaaggg tatctctttc ccgaaaccta ttcctttgcg aagggtacga
    38161 aggcgcggga ggtgattaag gcgatggtcg atggtctcca tcgtgtctgg ggtaccgagt
    38221 tgcaggagca ggcggcccgc atgaaaatgt ccctgcatca ggtgctgacc ctcgcctctg
    38281 tcatcgaaaa agaaaccgga gcgaagcacg agcgggaact gattgcggcg gtgtttcata
    38341 accggttgcg gaaaaagatc ccgcttcaga gcgacccgac ggtgatctac gggttaccgg
    38401 cgttcgacgg gaacattcac aagcgcgatc tctccgtcat gagcccctac aatacctatc
    38461 gtgtgcaggg tctcccgccg ggaccgatcg ccagccccgg ggcccattca ttgcgtgcgg
    38521 cgctgtttcc cgcccaggcg tcgtatttgt atttcgtgtc gcggaacgac gggacccatc
    38581 aattttcttc cacgctggcc gagcacaatc aagcggtgga gaaatatcag aaacagtact
    38641 tccgcaaacg tgccaggggc aaccttgtcg cccacggtgc gtgatcgtat tgagaaccgg
    38701 tcacgacacg tcctgtctca tttcctcatc gatcgtagaa tgtgaaaggg acattcgtat
    38761 gagtgtgaag tcgtggaatg aatcgttgcc gcgttacctg gaccatacgg tgctgcgtcc
    38821 ggaggctact aaggccgatg tgttacgcct ctgcgcggaa gccagggagc aggggttcgt
    38881 cgtgattttt gtgcctccct gttatgtgga tgaggcggtt gctgcggttg cagggaccga
    38941 tgttcaggtg ggcatcccca tcgggtttcc gctcggtggg cattcgactc acgctaaggt
    39001 aacggaggcc atggaggcgg tggcgcatgg cgcgcgggtg ctcgatatgg tgatcaacat
    39061 cagccgtttg aagtccggcg attacgatgt ggtgcgaaac gatatggccg ccgtcgttca
    39121 agcgacgccg ggtgtgaatc ataaggtgat tctggagact tgcctgctga cgcgagaaga
    39181 aaaaatcacc gcctgtcggc tggccgtcga ggccgggatg gactatgtga agacctcgac
    39241 cggattcaat caggccggag cgacagtgga agacgttcgg ctgatgaaag aagccgtggc
    39301 cggacgggcc aaggtgaagg cgtccggtgg aatcagagat tggaagacca cgcgggaatt
    39361 gctggaggca ggggccgacc ggatcggcac tagcgcgagt ctgaagattc tgagtgaatg
    39421 gcgcgccgcg ctggttgcgg tgcgatgaac cgccatccgt gtgaaagcct tgctcgttcc
    39481 ggtggatttg gtatagtgcg cccatgatga ctcgaatcgt attgctggtg ctcgatggtt
    39541 tcgggatcgg tgctttgccc gatgccgacg tgtatggcga cgccggctgc aacaccctgc
    39601 agcggctggc tgccatttcg aaggggctcg ccctgccgaa ttttgagcaa ctcggtctcg
    39661 ggcatctggg ccagtttcaa ggtatccgcc cgatggtgca gccggaaggc tgctacggga
    39721 ctctcgggtt ttcgaccaag ggaaagaatt cgctctccgg gcattgggaa atcgccggat
    39781 acgtgatcga agaaggcgag cgcccctgtg aaaccttcac gaccgagctg gcgaatgcgc
    39841 tcgaggccgc gctgggacag aagaccctgg gcaattgccg tgcggtcacg caggaaccga
    39901 tcgccgagtt cggcggacag catcagaaat cggggatgcc catcgcctgg atcgatacgg
    39961 cggggactat ttttctggcg gcgcacgagc aggtggtgcc gcctgaagaa ctctatcgcc
    40021 tggcccgtga agcccgaaag ttgctcaagg gcatggtgcc gattgtgcgt gtggtcgcct
    40081 gccccttcgt cgggcaatca ggcaagttta ctgcgacgga gcggcggcgg gattttgccg
    40141 tggagccacc ggggctgacg ttactggatc acttgagtcg ggccagccag ctcgtcatcg
    40201 gggtaggaaa ggtcggcgac ctgttcagcg ggcgcggggt gacgagatcg gtgccgctgt
    40261 tccaggcgga agccgtcatg gatgaagtgg tgggtatgtt cagcaaggtg ccgcgtggtc
    40321 tgatttacgc aagcctcccg gtcatcaatc ccgatcttca taccacagtg tctacattgc
    40381 agcagatcga tcgtcggctg cgggatttgc aggaatccct caaggtcgga gacgtgttga
    40441 tcatcaccgg cgatcacggc ttcgactgtg cgaggccgaa tcccggtcat tcacgcgaat
    40501 atgtgccctg tctcgtgacc ggtccgcgtc tcgcgcgcgg cgtgaacctc ggaaccagat
    40561 cgaccgcggc ggaccttggc caaaccatcg gtgaagcgtt gggcgcgacc agactgccct
    40621 ggggcgacag tttcctggat gcgctgcagt ctcgttagcg gacgcgtctt catcagtagc
    40681 agcgacgagt gaaaggattg cctgtgtcga tcgatgcaac attgaaatct ctcgggatcg
    40741 aattgcccgc tccgccgaag ccggtggcga gttatgttcc agccgtgctg gccggtgatc
    40801 tgctctttct gagcggcatg ttgccgtttc gtgacgggca ggtcgcaatc acgggcaagc
    40861 tggggcagga cgtgacggtt gagcgcgggg ccgaagcggc caggctggcg ctgctgaatg
    40921 ccttggcggt cgtgaagcag gagctcggtt cattggatcg tgtgcggcgt atcgtgcgtg
    40981 tcgtggggca tgtggcatct gccgcagggt ttgtgcagca gccggctgtc atcaacggcg
    41041 cgtccgatct gttggtgcag gtgttcggcg aggccggacg ccatgctcgc gtggcgctgg
    41101 gtgcggcaga gttgcccttg cacgccgcaa tcgaactcga attactggtc caggttcgac
    41161 cataaaacac tttgctccgt ctgttccctt cctgtcgcgc ccgcttgcct tgacatcaaa
    41221 aaaatccgct tgttatagtc cgtacgcccc ttcgttcatt gccgtacccc ccatccggcc
    41281 tcgctgcctc agttgcgtgg ggttttcgtg ccacgtgcgg ggcgttcgtc tgatgtgtca
    41341 tcatttcagc ctcgcccgag cctgctggtc aggcctgccc cgaggctttc ggtagggagt
    41401 gtcatcccat gcaatacatc gctcgtctcg tgctcggtgt cgcgctgttc gcaggcccgg
    41461 tcgtttccgg agaagttcgg gcagaggggc cggttcttca tcccaatgct gccgtgccgg
    41521 gatcgaccct ttcgatcacc gggaaggggt tcggccccta taaatcgacg aagtttaatc
    41581 aggtgacctt ccagggcgtg ccggccttga tccaacgctg ggaggccgat ctcatcgaag
    41641 tgcgcgtccc ttcgcaggcg gccaatggac cagtcgacgt gatcatcggg aagaagcacg
    41701 taaaggccgg atcgtttacg cgattgcagc ccgccattca ttcgttgtcg ccggctgagg
    41761 ccgaaccggg aacgattctg gagatcacgg gcgaacattt cggcaacact gccggcccgc
    41821 gcgatccgaa cacgattttt ggcgtcaata ctgtggccgt tggcgatgtg acggtgcgtg
    41881 tccgtaagtg gcgtgacgac aagattgaag tggagctgcc gggtaatgtg cagtcgggtg
    41941 atgtggtgat tcgcatggct tcgtccgatc cgttgccgga cgggtcttgc tgtgcgccgg
    42001 tcaagcaggt ggtgagcaat tcgatgccga tgaaggtgct ggcgtcggtg agggtcgatc
    42061 ctacgagtgg gccggtcgga acgaaggtgg tgctgttcgg caaagggttc gggacgtcca
    42121 ggaaccctga ggatggcgtg ctctttggcg ggcatctggc caccgtgtcg cagtggacgg
    42181 atacgaccat tgtggtgcat gtgccgctcg atgcccaaac cggcccggtt gtgatgaagc
    42241 gcaatgggca ggaacgggcc atcgggacgt acactgtaca gacgccccag gccaccgggc
    42301 tgactccggc cgaggcacct atcggcacct tgttgaaaat cacgggagag aattttggat
    42361 tttattcgga ggccgggtct acgcccttta actatatcga tttttcgttg agtgaaaaca
    42421 ctgtcgagat cggtggggtg caggcgatcg tgtaccgatg gggccacgac cgcattgatg
    42481 tctgggtgcc cttcagcgcg aaaagtggtc cggtggtggt caaacgtgct gccaatgctc
    42541 ccaagccgga tggcacgtgc tgcgccgata agaaagtgct tgaaactcag gtcggcaatt
    42601 ttacgctcgt cacgccgaaa atcgattcct acagtccgac caccggggga ttggatgaag
    42661 tggtgacgat caaggggagc gggttcggga agtttctcaa gacggccgaa ccaagcaagc
    42721 taatcacgga tagcgtctat gcccgggttg ctcccgtgct gggagagaac gtatcccgca
    42781 ccgaagtgtt gttcaacgga gtcggggcga tcgtgcagtc ctggacggac acggaaatca
    42841 cagtcaagat tcctcatcgc cattcctatg gcgtgggcaa actcggcgag tttaatccgg
    42901 acctgacgtc cgggccgctc gtggtccgtc gtggatcgtg ggatctcctg ccggacggct
    42961 cctgttgcac cccaaagaag tggcttacgc tcgaggccgg cacgtttacg atccagccta
    43021 ccggactgcc tgatgcgagt tactggcgga atcaaagttc agagaatact caccaccaac
    43081 aatgaggcgt cgtacgttgc atattctggc agttgtatcg tggttgttcg tcgccctcct
    43141 gtgcgcaggc acggccgagg cggcttcccc ggagagccgg gctaccgtag ctatgcagaa
    43201 aagtgggtct gaagccacac tgctgggcat gttctttcac gatcctcaac ttggctgggc
    43261 cgtggggtcc ggcggaacga ttctcaagac aaccgacggc ggccgcaaat ggaagaagct
    43321 ctccagcggc accacgtcgc tgctgacggc cgtgtatttc caggatgcgc gtcgtggatg
    43381 ggttgtgggc gccaacggca ccgtgcggac cagtcgggat ggtggagaaa cctggagcgt
    43441 cgtgtttgtg tccacgcagg ccccgctcta tgggatcgcg ttcgtgacgc cccagaaggg
    43501 ctggttggtc ggcggcaatg gtacgattct gcagacgtcc gacggcggag agaattggac
    43561 ggatcaagcc agtggaacga gcgccgcgct gcactccatc gtgttgacga accagcagca
    43621 cggagccatc gtcggcgcgt tgggcacgat tctcacgacc tccgacggcg gtgcgagctg
    43681 gacgccccag gtcagccaga gttcggccac gtttttcgat gtggctttcg cggatgaggt
    43741 gaacggctgg gcggtcggca atgccggggc gttgtttcaa acgaccgacg ggggcaccca
    43801 ttggattgat cgaacgttac cctgcggcag gacttgtaac aagctgaccg acctgattcg
    43861 ggtccgtttt accgatgccc aacaaggctg gatcgtggga gagcatggtc agattttgcg
    43921 aaccaccgat gcaggattta attgggtgga agaagccagc cccgtgaaac gctcgctcat
    43981 ggccttgagt tttcctcaca ccaccgccgg gtgggcggca ggcgaggggg gcaccattat
    44041 ttcgatcaac cccggccgtc ggtagcgctc gccagatccc gatcctaccc cagtcggccc
    44101 aatcgactct gcacaatact cagtgcagta actgctgcgg tgtccgcacg gaggatacac
    44161 gagccgagac tcacggcctg gcactgctga gccagggcca gagtgatctc ttcttctgcc
    44221 catcctcctt caggtccgat catcacggtg agctgttccg aggggagact cggcaggggt
    44281 atcctggcca atgcggcagc ctcatggcgt tctgccagga gaagcgagca ggtcgctttg
    44341 tgagtggaga agaagcggcg tgactccagc ggttccaaca cttccggggg ctgccactgc
    44401 tccgattgtt gcgccgcttc ggtggcgatg cgctgccagc gggccacttg ggcggcgatc
    44461 ctgtccggct gcggtcgtac gaccccatgc cgtgagacga ggggaaggat cgtccgcacg
    44521 cccaactcgc tcgccttttg caccacccaa tccatatggt cgcctttgag cagggcttgt
    44581 gcgagcagca ggggggggcc ggtctcgacg ggtcccagcc gttgttcgag aatctccgtc
    44641 atcaccgcct gctgggatac gtgcgtcacc cgcacacgat atcgtcgccg ttgttcatcg
    44701 gtgagccaga gatcttcgcc gggtttgacg cgtaggctgg cccgcacatg atggcagaga
    44761 tcgcccgtga gggtgatctg ggtttcacga atatctaatg aggagacgaa gaaagccggc
    44821 atggtggtcg gcggaacagg tggggcggtg atcggtctat tcgaagaacg ttttcatctt
    44881 gtcgagaaag ccttcgccct cggtgtcttt cgtgtagccg ctttccttgg caaattcttc
    44941 aagcagctct ttttgctttg aattgagctt ggtcggaatc tcgactttca cgttgaacag
    45001 ctgatcgccg gtatggccgc ccttgaggtt tggaaagccg aggcctttga tgcggaggat
    45061 tttgtccggc tgcgtgccgg cagggatctt catgaaggtg ttgcctttga gcgtcggcac
    45121 ctccactcgc ccacccagga tggcggtgac gagattgacg gggagttcgg acacgatatc
    45181 ctggccttcc cgcctaaaat gaggatgggg cttcacggtg atggcaacat acaggtcccc
    45241 ctgagggccg ccctggacgc cgtgttcgcc ctcgttcgcg agacgcaggc gcatgccggt
    45301 ttcgatcccg ccgggaattt gcacggagag catgcgttcc ttgcggatac gctgtcgccc
    45361 gccgcaggcc tggcagggtt cggtgatgat ctgtccgacc ccttcgcatt gaccgcaggg
    45421 ccggctgacg ctgaaaaacc cctgttggaa ccggagttgg ccctgtccct tgcaggccgg
    45481 gcacatttta atggccgtgg ctgagcgcgc tccggtgcct ttgcaatcgg cacagatttc
    45541 ccagcggggg attttgagtt tcgcttcctt tccaaatacc gactcttcaa acgtcacctc
    45601 aaggttgtat tgcaggtcgt tgccgcgctc ggcgcgggct cgtccgcgag gctggccgaa
    45661 gaagtcttcg aaaatatcgt tgaagatgtc gccgaatccg ccctgtccgc cgaaatcgaa
    45721 tccctccgct ccctgcggtc caccggcatg gccgaaggca tcgtatcgcc gccgtttttc
    45781 ctggtcgctg atgacctcgt aggcttcgtt cagctctttg aatttttctt ccgcggattt
    45841 tttctgttcc ggcgatgtgt ggagatcggg gtggtgctgg cgggcaagtt tccggaatgc
    45901 cttcttgatc tcgtcgtcgg aagcggttcg ctcaatgccg agagtttcgt agtagtcgcg
    45961 cttagccacg gaagattctt acccggttaa atgtgcaaca cgaccgagct tcacgcatgg
    46021 tgaaactcgg tcgtgcaatc ggtgagggtc ttactttttg tctttgtcga cttcttcgaa
    46081 ctccgcgtcc acgacctttt catcggtctt ggcgtgcgcg ccgccgtccc cggaggaagc
    46141 agctgcatca gccccggcgc cggcaccggc tgcggcagag gctttcttgt acatctcctc
    46201 agccagttta tgggatgccg tggtcagcgt ttgcgtggcc gtttcgatgg ctgcgggatc
    46261 gtcgccttcc atcgccttac ggagtccggc gatggcttcg gtgatcttgg tcttatcgtc
    46321 ttcgccgatc ttatcgccat gctcgctcag gttcttttcg gtgctgtaca gcaacgagtc
    46381 ggcctggtta cgcgcttcag ccacccgacg acgtttcttg tcatcctcgg tgtgggactg
    46441 ggcgtccttg acgagcttat cgacttcgtc cttgctgagt ccgctcgagg ccgtaatctt
    46501 gatggactgt tccttctgtg tggccaggtc tttggccgcc acatgcacga taccgttggc
    46561 atcgatgtcg aaggagactt cgacctgcgg catgccgcgc ggagcgggag gaatgccgac
    46621 aagatcgaat tgtcccaaca gcttattgtc gttcgccatt tcacgttcgc cctggaatac
    46681 gcgaatggtg acggctgtct ggttgtcggc agcggtcgag aatacctggc tcttcttcgt
    46741 gggaatcgtg gtgtttcgct cgatcaactt cgtaaagacg ccgccgaggg tctcaatgcc
    46801 gagcgacaag ggcgtgacat ccagcaacag cacgtccttg acttcgccct taagcacgcc
    46861 gccctgaaca ccggctccga tggcgacgac ttcgtcgggg ttcacgccgc gatgcggttc
    46921 tttgccgaag aattccttca cgacctggat gactttcggc atgcgggtca tgccgccgac
    46981 gagcacgact tcttggatgt ctttcgcgct cacaccggca tccgccaaag ccttccggca
    47041 gggctcgatg gtccgttgga tgaggtcgcc gacgagttgc tccagctttg cgcgggtcaa
    47101 cttcgtgacc atgtgcttcg ggccgctggc gtccgccgta atgaacggca ggttgatctc
    47161 gctttcctga gacgaggaga gttcgatctt ggcccgctcc gccgcttcct tgaggcgttg
    47221 caacgccatc cggtctttgc gcaggtcgat accttgatct ttcttaaact cctcgacgag
    47281 ccagtccatc acacgctcgt cgaagtcgtc gccaccgaga taggtgtcgc cgttcgtgga
    47341 cttgacctca aacacgccgt cgccgatttc caggacggac acgtcgaacg tcccgccgcc
    47401 gagatcgtag acggcaatgc gctcgtcttt tttcttgtcg agaccgtagg ccagtgaggc
    47461 cgccgtgggt tcgttgatga tgcgcaggac gttcaatccg gcgatctgcc ccgcatcctt
    47521 ggtggcctgg cgctggctat catcgaaata ggccgggacc gtcaccactg cttcagtgac
    47581 cttctcaccc aggtaatctt cggcggtctg ccgcatcttc tgcagaatca tggcggagac
    47641 ttccggcggg ctgtaacgct ttccgcgcaa ctcgacgtgt gcatcgccgt tgtccgcctc
    47701 caccaccttg taggggagcc gcttcatggc ttcctgcact tctttgctgc ggaacttgcg
    47761 tcccatgaga cgcttgactg agaaaatggt gttttcagga ttggtgatgg cttgccgctt
    47821 ggcgatttgt ccgaccaggc gctcgttctt atccgtgatg cccaccaccg acggagttgt
    47881 gcgacttcct tccgcattgg cgatgacgac cgggtctcca ccgctcatga tagcgacgca
    47941 ggagttcgtc gtgccgaggt cgattccgat aactttgccc atgggttggc tccttcgcaa
    48001 aaaatatcaa tacatgaacg gtcgtgtcag ttgtgcttgc gaaacctgtt cttcacaccg
    48061 atgattcggc cctagtttgc ggctccggtc gacacggtca ccatggcggc gcggaggatg
    48121 cggtcctgga ggagatatcc cttttgatac tcttccacga catggttttc agggatcgtg
    48181 tctgacggaa cctgggcgac tgcctgttgc gtggccggat caaaggccag gccgacgctc
    48241 tccactgctt tgacaccgaa cttcgtcagc gcgccaacga gttgcttcag cgtcaattcc
    48301 acaccctctg tcaacgcatc gacggagccg cttcccttcg acgacttaat ggcgcgctca
    48361 agattgtcca ccacgggtag aagttccttg aggatctgtt cgttcccgaa cttgatttgc
    48421 tcacgttgat cgcgttgagc cagcctcttg tagttgtcga actctgcggc gagccgcaaa
    48481 tacttttcgt tgagggcttt gcattcgtcg gacttggcat ccagcacctg ctggagttca
    48541 ttgacaccct ccatcgtgcc ggaagatgcc tcgctggaac cgtctaagtt gtcgatactg
    48601 tgaatattct tgtcgtcttg agacattgat tacacctttg ctggggagtg agatagccac
    48661 cggagaacgg aagtcaacgg ggaatagaaa gaaatcatcg ttttttcagc tctctaaggg
    48721 tttgaggcga ggggtaacgt cagtctgcgg tgagccaggc ctaggtcagg cggtttcgat
    48781 ggtacaggag ttccaagcct tcaagcgtca ggaaggggcg gacttcttgg atcgtttcgg
    48841 tctcctgggc gatgaccgtc gcgagccctc cggtggcgat gacggtcgat gtccgtccga
    48901 gttctcgctc catgcgtcgg acgataccgt ccaccagccc aacatagccg aacagcagac
    48961 cgctttgaat gccgccgatc gtatcggtgc cgatgacagt tttgggccgg atgatttcca
    49021 ccttcggcaa cttggcggta cgggaaaaca gagcgtcggc tgaaattccg agacccggtg
    49081 cgatgacacc gccgagatac tcggcggatt tggtcacggc gcagaaggtg gtggcggttc
    49141 cgaagtcgac gataatgaga tcggaccggt accgcgcata agccgcggcg gcgttgacga
    49201 tgcggtcgct gccgatttcc ttcgggttgg cataccgcaa ggtgagcccg gaatcggtat
    49261 cgggtccgac gatcaccggt gttcggtgaa agtaggtttg caccagggag tcgaaggtgc
    49321 cggtcagcgc gggcacgacg ctcgagagaa tgcagcccgt aatctgctcc ggggtgaagc
    49381 cggcgttgtt gagcagattg agcaacagaa tgccgtattc attgtcggtt cgccgtgatt
    49441 cggtggcgag gcgccaatgc ccgcgcaggg tggaatcttc gaacaggccc cacaccacgt
    49501 tggtatttcc gatgtcgatg gtcagcagca tcgtgtctcc actctacccc aacaggttgt
    49561 ccgatttcac ccccgcacgt ggatgacttc cgcgcttcga acttccagca gccggtgagg
    49621 cagagtgggg gagggatcgg aggtcacacg caaacagagg catccatccg ggccgatcga
    49681 ctcggcgatc ccctggacga tcccgttttc ttccagagcc acacggacgg tcttgccgag
    49741 tgtggagcag cgtcgtgtga attcatcgat catgccggac ggtccgtcgt ggaacaaacg
    49801 atccatgcgt tgttcaagcc ggagcaagag atctgcgagg atcatggcgc gatcgagccg
    49861 atggcctgcc tcggcagcca tggtggttgc gccggctctg agctcctccg ggaaactgtc
    49921 gggtgcagcg ttgatgttca atccgatgcc gatgactatc gccagggttc ggtcagcggt
    49981 ggtggtttgt tcgcagagga tgccgccgat tttcttgtcg ccgatcaaca gatcgttcgg
    50041 ccacttcacc gagactgcca gtcccgtgca gccggagagg caatcggaca ccgccagagc
    50101 ggaaaacaac ggaatccagg agagccaggg ccctatgcgt gtggagccgg gttcgggcac
    50161 cacaatcacg gacatgtaga tgttgccctg ggccggagag tgccaggcgc gtccgcggcg
    50221 tccccgacca gctgtttggc agtccgcgag gatcaccgtt ccgtgcgcag ccgctggtcc
    50281 ggtcggttgt tggaggtatg ccaacgcgtc tgcattggtt gaagccgtgg aaggaatgta
    50341 ccgcagcgtc tgtccgaagg tgtgggtgtg caggccggct tgaaggcgat caatgcccag
    50401 ccgatcggct ggatgcggat cgttagctgg tccgcccacg gctccctcga tagccggtga
    50461 ggtccatgct gaggtcggct gcgggtgcgg agtgagtcag ggcaccgacg gaaatgaagt
    50521 ccacacccgt ttgggccatg tcggcgacgg tctgcagggt gatgccgccc gagacttcta
    50581 ccaaggcgcg ctttttgatg agggccaccg ccttttgcac gagggccgga gacatattgt
    50641 cgagcaggat gatgtcggcg tgaccagcca acgcttcctt cacttcttgc aaactcttgg
    50701 cttctacctc gatgcgaagg ccatgcggcg ccccggcgcg tgctaaccgg caggctccgg
    50761 ccacatcgac gccggttgag cgcaagactg cgagatgatt gtccttgatc aggacgccgt
    50821 cgcccagtga aaagcgatgg tttctcccgc ctcccaggtg aaccgcccat ttttcgagtg
    50881 cccgcaagcc cggtgtggtc ttgcgtgtat cgagaatggc ggtctccgta tgtcggattg
    50941 cggcacagaa ttttgcggtc aacgtggcga tgccggagag gcgttgaagg aaattgacgg
    51001 cgacgcgttc cgccatcaat aacgaccgca catcgccccg gatgacgatg acctccgcgt
    51061 cgggtttgac cgtggctccg tcgttgatgc tcttgacgat gcgtaacgaa gggtccacag
    51121 cgaggaagac ttcgcgcgcg acgaccacgc ctgccacggt gatgggttga tgggcgacga
    51181 tggcggccct ggcctgaatg gaacagggaa acagcgcact cgtggtgacg tcgccgtgag
    51241 caagatcctc tgctagggcc gcctgcacga cggcgcggag ctgggtatgc ggaatagcat
    51301 tcatgggcca agctcggcca gcatgctacg gatttgggcg tcgctgcttt ccaggatggc
    51361 ttggtccttg taaagggttg acagacgctg gcgctgctca actacgactt ctggcggggc
    51421 cttggccacg aactctttgt tgccgagttt tccctccaat cgctcagctt ccttcttccc
    51481 gtcctcaagt tgtttcttga ttcgggcgag cactttctgc agttcgacat ctccctcgac
    51541 gttgacaccg acggacagcc catcggtcac aagccttagt gtacgctgag ttggccaagc
    51601 cgcatggacc tgcacgctcc cgcgaacgag atgggcaatg tggtctcgca ggtaatcaag
    51661 gggggctgga ttggacatat ctgggccgaa aacatggaac tcaagcactt tcgcaggcgg
    51721 ataattcaag agcgaacgcc ccattcgcat ggtgctgacg ctcctgtcga gaagtgcaaa
    51781 caaaccctcg gcgtcatgtg agctccattc tggttgcgcg actgggaagg gctgtcggac
    51841 aatgctgtcg ccttcgtgcg gcagggtctg ccagatttct tctgaaataa acggcatgaa
    51901 gggatgcagc agacgcatca tggtttcgaa ggtctcggcg agggtctgtc gtgtcgattt
    51961 ggcctgttcc gatgcctgat ccttgagcgc gggcttgatc aattccaagt accagtcgca
    52021 gtactcatgc cagatgaatt ggtagatcgc gctcgacgcg cggtcgaagc gatactgctc
    52081 caactcctgc gtgacggtgc cgattgtggc attcaggcgg ctcagaatcc aacggtcggg
    52141 gaacgaccgc tgcgcggcgg gcatctcaag ccgttcgccg tccagatgca tcaggagaaa
    52201 ccgggcggcg ttccatattt tgttcgtaaa gttgcggtag ccttcgatcc gttcttccgc
    52261 cagcttgacg tcgcggcccg gtgaagccat tgcggccatg gtgaaacgca gggcgtcggt
    52321 cccgaactgc tccatcacat gcaagggatc gatcacattg cccttcgact tactcatctt
    52381 ctggccctcg gcatcgcgaa ccagggcgtg gatatagacg tcgcggaagg ggacgtcgcc
    52441 catgaacttc aggcccatca tgatcatgcg cgcgacccag aaaaacagaa tgtcgagccc
    52501 ggtgaccagg gtcgaggtgg ggtagaaggt cttcaaatcc ggcgtctgct tcggccagcc
    52561 cagggtagaa aatggccaga gggcggaaga gaaccaggtg tcgagcacgt cggggtcctg
    52621 caccaacgca tctgagtggc ccttgggaca ggttgccggt gtggtcttcg ccacgatgac
    52681 ggttgcgtcg gaaggaatga gcggcgcccc gtctgacgag cggcgcagaa acgggcttcc
    52741 gtaacaggtt tcacagtacc aggcaggaat ctgatgaccc caccagattt gccgggagac
    52801 gcaccagtcc ttaatgtctc gcatccagcc gagatagttg ttcttccagg cctcgggaat
    52861 gatcctgacc cgaccatctt cgaccgcttg aatcgccggt tccgcgagcg gcttgatttt
    52921 gacgaaccat tggtccgaca acagaggttc gacaacagtc ttgcagcggt agcatttgcc
    52981 gagcgccatc ttatggggct cggatttttc aagcaggcct tgatccgtca gcagttgttc
    53041 gatcttcggt ctggccttgg cgacaggaag gccttcgacg atgtgcgcca cctcggactc
    53101 taccagtgcg ccttccagcc gaaggttggc gtggatgtcc aaaatcttga tgcgccggag
    53161 cccatgccgc tctcccgcct caaagtcatt aaagtcatga gcgggggtaa tcttgacggc
    53221 gccggtgccg aattcgcgat cgaccagaat cgagtctcct acgattggaa tcgcgcgggt
    53281 cgtgaggggg agccggacct gtttgccgat gaggtgacgg taccgttcgt cttccgggtg
    53341 caccgccacg gcggtatcgc cgagcagggt ttcgggacgc gtggtcgcca ccagcagcac
    53401 ctgcgcggga tcgtcggcca gggcataacg aatggtgtac agttttccgg taatctcttc
    53461 atgttcgact tcgatgtcgg acaaggccgt caggcagcgc gggcaccaat tgatcaatcg
    53521 ttcgccgcgg tagagcagtc cgtctgcatg cagccgaacg aagacctcgc ggacggcttc
    53581 cgaaagcccc tcgtccatgg tgaagcgttc acgttcccag tcgcaggagg cgccaagccg
    53641 tttcagctga gagataatcg tgccgccgga cttttctttc cactgccaca cccgcttgag
    53701 gaactcttcc cgaccgagtg cctcccgcgt ggtgccttcg gcctgaagct gccgctccac
    53761 cacgttctgg gtcgcgatgc ccgcgtggtc cgtgccgggc atccaaagca catttcggcc
    53821 ttgcatgcgg cgccaacgga tcagaatgtc ctgcagggta ttgttcagcg catggccaac
    53881 gtggagcgat cctgtgacgt ttggcggagg gatcaccatc gaatagggct gcccgggatg
    53941 ggccgagtcg gcatgaaaca gaccggcgtc gatccagcgc tggtaccagc ggtcttcgac
    54001 gtcgtgggga ctgtaggttt tgtcgagttg cggtgtggac atggttcttg gaggcgcccg
    54061 gggcgcacgc gggcgcatct taacatgcgc cgtgtgggct cacaaggtaa tggacgcgcg
    54121 tctgtgatcg ttctagcccg gattattcat gaatgtcgtc gcgaggcgtt cgagaccgaa
    54181 gcccgcc
//
LOCUS       DAORRQ010000025        53346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_36_length_53346_cov_125.580867, whole genome shotgun sequence.
ACCESSION   DAORRQ010000025 DAORRQ010000000
VERSION     DAORRQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 53346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53346
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36_length_53346_cov_125.580867"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK224_09795"
     CDS             complement(<1..350)
                     /locus_tag="PK224_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16071.1"
                     /translation="MNLQLSPLIELQKLDLRIADLKEQRRKIPERLEENEAPLREAKR
                     LFREASASVESLVKDRRGHEKDLEAHEDRIGKMKDRAAQLKTNQEYQAHLFEVELANK
                     KRGEIEEKILLAME"
     gene            complement(428..504)
                     /locus_tag="PK224_09800"
     tRNA            complement(428..504)
                     /locus_tag="PK224_09800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(468..470),aa:Ile,seq:cat)
     gene            complement(659..2464)
                     /gene="rpoD"
                     /locus_tag="PK224_09805"
     CDS             complement(659..2464)
                     /gene="rpoD"
                     /locus_tag="PK224_09805"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRB16072.1"
                     /translation="MPKQELLGEVKKLINLGKEKGFLTYDDLNSTLPADVVSSDQFST
                     IMTMFGEMDIEIVESAETERAPKAAEGEEAIEESEEAEASEENEKEIDLTPGALSRTD
                     DPVRLYLKEMGSVALLSREGEIEIAKRIEEGKKDIATVVYGLPMTLEFVLNLRDQLKN
                     GKIDVREIVPVVETEEDFEEEAVEKDYEELRVKTLDSLNAVRKVSASLKDLYDKARHV
                     GADPEKQKKLRKQIDTVRGQVVDKMESVNLHGVLKDRMTQRVRDLNLLFRQAEREVTS
                     CQRRLGVGGEAGAELLRRLCRTHKDLLAVKRRTSLSEEVLQDIKKHYQAAKGRIRQLE
                     TEEALISAEEIKDAVKHLDVAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFLDLI
                     QEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKL
                     IRTSRHLVQKLGREPTPEEIAERMDLPLDKVRKILKIAREPISLETPIGEEEDSHLGD
                     FIEDKKAVSPLEAAIRYDLQRQINGALETLTPREEKVLRKRFGIGEATDHTLEEVGQD
                     FEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESL"
     gene            complement(2467..4311)
                     /gene="dnaG"
                     /locus_tag="PK224_09810"
     CDS             complement(2467..4311)
                     /gene="dnaG"
                     /locus_tag="PK224_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRB16073.1"
                     /translation="MGQGLISDDVINQIRDRVDIAEIVGQHVALTRAGQNLKGLCPFH
                     QEKSPSFTVSSSRQIFHCFGCGAGGNVYTFLMKLTGAGFPEIVRDLGRKVGVDVPESA
                     GGYSSEARTQLGRLERLNAAAGAYYQRMLLDNTAGAPARAYLDGRGIQHNTMAQFQIG
                     YALPEWDGLTKAMLKEGFAPADLVAAGLSIPRDQSGQQKAAVSGHYDRFRARVMFPIM
                     DLRKRVVAFGGRILGEGMPKYLNSPETPLFKKGQTLFALEAAREAAGQQHTLIIVEGY
                     FDAVALHQAGIRHVAATLGTALTAEHITVIRRFASNVVLLFDPDQAGVRAALRTLDLF
                     VNSGLSVKVVSLPEGDDPDTYVRKYGAEGFTALHEAAPSLLDFAVEHSLRTAESGTVE
                     DRIRAVDAVLRILQKSAHPIEREERIRLVAERLGLSQQRLIDRYPTLASEETRRPSSR
                     PTALPAAAASKPKGNPEERDLAHLLVQGSLSAEDLRKLSPDAFSAPAYRRLIECAMQH
                     LEQDGRVSVRGLLDALINDENCGSLVSELSMLEQHYDDVPAHIAGCLETLERRGRERT
                     MGALIQELKAAERERREADVHRLNGLINEMRIRKAGVMPAALTSAVKE"
     gene            complement(4443..4787)
                     /locus_tag="PK224_09815"
     CDS             complement(4443..4787)
                     /locus_tag="PK224_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRB16074.1"
                     /translation="MDNCIFCRIVEGGIPAKIVYQDDQVLAFDDINAQAPVHILVIPK
                     RHVAAVQDCREGDQALLGHLLLTCSKIAGMKNLAESGYRIVTNTGAESGQTVFHLHLH
                     VLGGRHMAWPPG"
     gene            complement(4780..5463)
                     /gene="hisIE"
                     /locus_tag="PK224_09820"
     CDS             complement(4780..5463)
                     /gene="hisIE"
                     /locus_tag="PK224_09820"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRB16075.1"
                     /translation="MSKESRTMTFDGQGLIPAVIQDWLDGTVLMVGYMNQEAIARTLQ
                     TRSVHFWSRSRRKLWEKGETSGHFLKVKDLFVDCDRDTILVKAEPVGPTCHTGERACF
                     FARMTEEGQASEEKTQDAGGGILDRVYQTILTRKASPQPDSYVSKLLQGGADRILKKV
                     AEEAGEVIIAAKNQKREEIIYETADLLFHTLLVLGYHDVTLNEVYRELGTRFGKSGIR
                     PSPEGGSRG"
     gene            complement(5479..6249)
                     /gene="hisF"
                     /locus_tag="PK224_09825"
     CDS             complement(5479..6249)
                     /gene="hisF"
                     /locus_tag="PK224_09825"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRB16076.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEVAAIYDREGA
                     DELCFLDITASHENRKTIIDVVEQTAARVFMPLTVGGGVRTLDDIRTLLNAGADKVSI
                     NTTAVQQPEFVREAAQRFGTQCIVVAIDAKQSGQAGRWEVFTHGGRKPTGLDAVEWAQ
                     RMEGYGAGEILLTSMDQDGRQNGYDLALTAAVSERLSIPVIASGGVGTLEHLYDGLVK
                     GKADAVLAASIFHYRTHTIQQAKAYLRDRGVPVRLGPA"
     gene            complement(6317..7042)
                     /gene="hisA"
                     /locus_tag="PK224_09830"
     CDS             complement(6317..7042)
                     /gene="hisA"
                     /locus_tag="PK224_09830"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRB16077.1"
                     /translation="MRVIPAIDLKDGRCVRLRQGDMAAETVYSEDVPSVAGRWQQQGA
                     DLIHVVDLNGAVDGEPKNLPQIEAVMKTVSVKVQVGGGIRTIETVRRYLHAGVARVVL
                     GTAALMDRTFLAQACQEFPRRILLGLDARDGKVAVKGWTAVSETKAIDLLKELAGLEL
                     GAVIYTDIARDGMLNGPNLTALREVVDCSSFPVIASGGITRVEDLQAVHALGPRVEGA
                     IVGKALYDGKLDYAAAVAAIGKR"
     gene            complement(7065..7784)
                     /locus_tag="PK224_09835"
     CDS             complement(7065..7784)
                     /locus_tag="PK224_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16078.1"
                     /translation="MRALLIPMTLLLLAGCSGTKVTSKASSQVGKYPVRTIALIPFET
                     MATPQAIEASGPAFPVPSGARRSDMAVGVPPTTDQAVRQTHRVPASAGEKVTDLMWAK
                     LKARSGLQLLSPNESARVAREVSGGTATETMPAHKIAQRLGADAALSGKVLVYQERVG
                     SRLGADPPAAVGFEVKLVAPDGIVLWEGNYYEKQRPMTEDFLGFVQRYGVFVTAEELA
                     AYGAEELADAFPYGGAAGGGK"
     gene            complement(7796..8464)
                     /gene="hisH"
                     /locus_tag="PK224_09840"
     CDS             complement(7796..8464)
                     /gene="hisH"
                     /locus_tag="PK224_09840"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRB16079.1"
                     /translation="MSAVLSVVHQVPTGRLRTQYMIAIIDYGMGNLRSVSKAFEAMGH
                     QAVVTREARVIADASHVVLPGVGAFGDCMANLERYELIAPIHAAVQSGKPFLGICLGL
                     QLLFTESEEFGVHAGLGIIPGRVKKFVLEPPLKVPHMGWNEIQIVQSAPPFAGLATGS
                     YCYFVHSYYVEPVESSVIATVTEYGRSFASAVWKDNVVACQFHPEKSQAVGLQVIKNF
                     GAWT"
     gene            complement(8492..9097)
                     /gene="hisB"
                     /locus_tag="PK224_09845"
     CDS             complement(8492..9097)
                     /gene="hisB"
                     /locus_tag="PK224_09845"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRB16080.1"
                     /translation="MKKNGAPRQASLHRATKETDIRVDWSLDGTGQGTVDTSIRFFDH
                     MLDLLAKHGFFDLTVQAKGDIEIDEHHTVEDVGIVMGKVLHQALGEKAGIKRFGFASV
                     PLDETLAQVTVDLSGRPYLVYNVNLPDRKIKTFDLGLFEDFFQAFVTHGGLNLHVNLQ
                     YGRNPHHIMEAIFKALARALDQATTLEERLSGTVLSTKGSL"
     gene            complement(9094..10377)
                     /gene="hisD"
                     /locus_tag="PK224_09850"
     CDS             complement(9094..10377)
                     /gene="hisD"
                     /locus_tag="PK224_09850"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRB16081.1"
                     /translation="MTILASTDRAYDKALRKIVTRARTQGAGVEKSVRTILKAVERGG
                     DRAVLRYTKKFDRLSLKLDHMRVTPEEIKEAYYHIRKDEGDSLRYAAERVRQFHERQR
                     TKTWMYQEQNATLGQMVTPLDAVGVYVPGGKAVYPSSVLMCAIPAKVAGVRRIVMCTP
                     TPKGEINPYLLVAADIAGVDEIYRVGGVQAVGAMAFGTKTIAKVDKIVGPGNIFVATA
                     KRLLYGTVGIDMVAGPSELLVVADDDAKPAHVAADLLCEAEHDEDAQVYLVTTSASLA
                     KEVVRLIQVQLKGLQREKIAAKSIARHFVAFVVPTMDEAIEVANAIAPEHLTLSVDRP
                     FDYLEQVRHAGALFLGRYTPPAVADYVAGPNHVLPTGATARFFSPLSVSDYVKVSNIV
                     HYTKEELTKAKDHIVRLAHIEGFDAHAKSAQSRFA"
     gene            complement(10428..11123)
                     /gene="hisG"
                     /locus_tag="PK224_09855"
     CDS             complement(10428..11123)
                     /gene="hisG"
                     /locus_tag="PK224_09855"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRB16082.1"
                     /translation="MGATKSHSKKSRGLTIALSKGKLLGPVLHLLKQAGYYSPGLSTE
                     SRKLVFACSSNDATFLIVRPTDVPTYVEHGAADVGVVGKDVLLEQGSDVYEPLDLKVG
                     ACRISVAALQGQPSPDRWSSKIRVATKYPNVTERYFNQRGVPVEIVKLYGSIELAPIV
                     GLADRIVDLVETGSTLKAHDLAEVEVITHSTARLIVNRASLKLKHEPLTVLIERLRAA
                     VAVEDSNGAASRA"
     gene            complement(11126..12382)
                     /gene="murA"
                     /locus_tag="PK224_09860"
     CDS             complement(11126..12382)
                     /gene="murA"
                     /locus_tag="PK224_09860"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRB16083.1"
                     /translation="MDQIIIQGGRPLRGEVRTSGAKNAALPILASTILGGGECVLSNM
                     PRVVDVLTMGKLLGMLGISVAQEVNHTVVQAQAIASTEAPYDLVRTMRASVLVLGPLV
                     ARLGEAKVSLPGGCAIGSRPVNFHLAGLEKMGATVEIEHGYIKATARRLRGARIYFDT
                     PSVTGTENLMMAAVLAEGTTVLENAAKEPEITDLAAFLIKRGARITGAGTDMITIEGV
                     TELHGADHAVIPDRIEAGTYLIAGAITGGEVVIDCCRPEHMEPLLMKLRESGVELIEE
                     KERIRLKVTGRLKGTNVRTSPHPGFPTDMQAQFVALMTVAEGTSVVTETIFESRFMHV
                     EELRRMGADIRVEGNRVVITGKERLTGAPVMASDLRASAGLIVAGLAAEGTTEVSRVY
                     HLDRGYERLEEKFRALGASVERRKGK"
     gene            complement(12388..13353)
                     /gene="prmC"
                     /locus_tag="PK224_09865"
     CDS             complement(12388..13353)
                     /gene="prmC"
                     /locus_tag="PK224_09865"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRB16084.1"
                     /translation="MSTEQLGIAATSAVTIRSMLAEAERSLHAAGIEQPALEAAWLLE
                     HVLHLSPLMQRVKAERPVPALDYARVLALVARRANREPLQYLLGTQEFCGREFRVTSA
                     VLIPRPESVLLVREAIRRCGQNPAAIVVDVGTGSGCLAVSVASALPDARVLAIDASAD
                     ALAVAQANMEQHGLDARIECMQGDLLAPLAECGFVSQIDVIVSNPPYIPDLDLVTLQP
                     EVRCFEPHLALAGGPDGMDVHRRLLQQAPVYLKSDGVLLLEVGLGQAAAVCREAEESG
                     WFRTYDVLRDEGGIDRVVCFEKKEEGRNPQSGQPSIQTATSRSSS"
     gene            complement(13350..14432)
                     /gene="prfA"
                     /locus_tag="PK224_09870"
     CDS             complement(13350..14432)
                     /gene="prfA"
                     /locus_tag="PK224_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRB16085.1"
                     /translation="MEDGLIKKWEGMAARFQELTNQLMDPSVLNQPGMIHKLNKERTE
                     LEEPALQFVAYCEAKKQLDEVSAMLQDASTGADMKQLFAEESDHLQQQVLDLEHRAKD
                     FLTPKDPRDEKNMFLEIRAGTGGDEAALFAGDLFRMYLKYAERNRLKLEVVDASETGI
                     GGYREVIALLEGKGAYGRLRYESGVHRVQRVPTTEASGRIHTSTVTVAVMPEVDEVEV
                     HIDPKDLRIDTFCSSGAGGQSVNTTYSAVRITHIPTGVVVSCQDERSQLKNRTKAMRN
                     LRARIVEAEREKQDAEIAQNRKSQVGTGDRSEKIRTYNFPQNRVTDHRIGLTVHKLDQ
                     VLAGDLEEIVQALRAHYEHVASLEVK"
     gene            complement(14528..14734)
                     /gene="rpmE"
                     /locus_tag="PK224_09875"
     CDS             complement(14528..14734)
                     /gene="rpmE"
                     /locus_tag="PK224_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRB16086.1"
                     /translation="MQKGIHPVYREATVHCACGNTFKTRSTIGNIKVDICAGCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKDK"
     gene            complement(14835..16082)
                     /gene="rho"
                     /locus_tag="PK224_09880"
     CDS             complement(14835..16082)
                     /gene="rho"
                     /locus_tag="PK224_09880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRB16087.1"
                     /translation="MHLAELKQKSIADLNEVARDLKIEGAANLRKQELIFAILQAQTE
                     KNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNYEDPEVARDKILFDNLTPLYPEERLNLEFDREEYCTRVMD
                     LTTPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALIDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRLFPAIDISQSGTRKEELLVDRDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKIGGTKTNQEFLQSMNR"
     gene            16595..17008
                     /locus_tag="PK224_09885"
     CDS             16595..17008
                     /locus_tag="PK224_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HRB16088.1"
                     /translation="MAKSDNDTPDRIFTLAEANELLPQLNQRFASVRRAKTVIASTKE
                     EVSKASAQAASGGGSPVGALYIRALHEISGSLQAIHELGVVIKDVDLGLCDFPYKLEG
                     RVVYLCWQFGEDEIRWWHETSCGYKDRHPLDETSS"
     gene            17027..17314
                     /locus_tag="PK224_09890"
     CDS             17027..17314
                     /locus_tag="PK224_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRB16089.1"
                     /translation="MTFVILGFDGPDGQAKRKIHRTAHLAGLEPLNRAGRVLLAGPFT
                     DQAGSLIIIEADSLEEAKRIAQEDPYTVHGIFERVEVHPFQQVLPASQSHT"
     gene            17648..18283
                     /locus_tag="PK224_09895"
     CDS             17648..18283
                     /locus_tag="PK224_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HRB16090.1"
                     /translation="MTQYRVLPGPEHFLPPAAASMGIYLPNPGEAHINGVIVPEEKAY
                     EEAARQFLMAQVPTIFPGPLVLWAWNEKAAKKAAAVRKLYEILKECVQPGQKPMLIPM
                     PDYRPKYPKINPEVEINPNHPNLTIWHNKIDCCMFIGVHCHQANLSLKIIRGGTSCYT
                     VAMCAQAGHEDAMLSFRDASVEKILKLGEWIRKLKGTVKPRLTTAKTSASN"
     gene            18326..19537
                     /locus_tag="PK224_09900"
     CDS             18326..19537
                     /locus_tag="PK224_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB16091.1"
                     /translation="MAEIKSHIGTQNKKGQTYTDAWEMMHEAPRNPSFYTGSEVIKEA
                     LRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTIEISYLLNTGML
                     VWHAETAQDFFDWILKGFMVSEEPEVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFIMKSNYISYATHASWQQEIWAAVERSRKHTIKWI
                     NGLIDTENTDAEVVIVASGTAVSQGREAIRLLEDEGVRCGLVKVKSLRPWPGEEIREA
                     TKNAKHIFVPEFNVTGWLAKEIKATIPNSERVHAGPHVCGGMTMPPEIIASEIKAALG
                     LRSESLAGRGS"
     gene            19607..20509
                     /locus_tag="PK224_09905"
     CDS             19607..20509
                     /locus_tag="PK224_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB16092.1"
                     /translation="MSKERIKISPDLYDIMPSDYQDLVQSATYGKEDRGWKDIGTSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPDRVKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVNCTVSKPSLVEKV
                     IRNSVLIAREIGPTYIQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAYKEYVSEPAK
                     QFLAELAAKDKERKAAAKQLAGKA"
     gene            20527..21234
                     /locus_tag="PK224_09910"
     CDS             20527..21234
                     /locus_tag="PK224_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRB16093.1"
                     /translation="MIKKRLNIRMSGLGGQGAVTAAHVMAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPDVIEVFHPQVITMGKSYTMPFYSGVKEGGVV
                     IINSAQPLLSEEDINRLKDLNVALFYIAGTELAIEVAGTELSTNMSMIGSVAGITKCV
                     SMEALDGALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKRAYEIASEW
                     ADKNKVELRVGNPAVAA"
     gene            21258..21536
                     /locus_tag="PK224_09915"
     CDS             21258..21536
                     /locus_tag="PK224_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRB16094.1"
                     /translation="MYNVAQVIDEKCVAKKGCRLCIMYCPEANCLDLNSTKMVAEVTI
                     DRCKGCELCVVVCNAAKHEAIEMQAVSATGQLMSHKGESAGLGQAYQG"
     gene            21614..21883
                     /locus_tag="PK224_09920"
     CDS             21614..21883
                     /locus_tag="PK224_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16095.1"
                     /translation="MEREDNVLDELLRDIAGLLNEYPKAIERQAAAIHAGGKDPELAE
                     KLIKAADTMRDSGNLYLTWAKHFAALAEGNTDASSDEDETDDFGI"
     gene            21948..22023
                     /locus_tag="PK224_09925"
     tRNA            21948..22023
                     /locus_tag="PK224_09925"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21981..21983,aa:Asn,seq:gtt)
     gene            complement(22096..22731)
                     /locus_tag="PK224_09930"
     CDS             complement(22096..22731)
                     /locus_tag="PK224_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRB16096.1"
                     /translation="MLTAIGDVMRRVYERGWITTRDGNISMRKRAGKYLYITPSGWRK
                     TIVHPEHIVRLEVLTDPVTGQLVPKVRDGQQPSGELWMHWNLQRDTKKTRTVVHVHAT
                     HIVAAIYAGIDLQAMSAEFPEISRYTRVGRTVPALPALSRDLADVTAECLGLKQDGTL
                     EFDIVGQMNHGVCAVATDPWGAYEHIERLDHICEIVLKSGVVSKPLPKQSS"
     gene            22979..23857
                     /locus_tag="PK224_09935"
     CDS             22979..23857
                     /locus_tag="PK224_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRB16097.1"
                     /translation="MTRRFTTEPRTQLQGFCHWQSHPRSSPTLILLHGLEGSADSHYM
                     RGMTTKAYRAGFNVIRMNQRTCGGSDHLTPTLYNSGLSNDYRAIIQELRERDHLSRMW
                     LVGYSMGGNLALKAAGEMGQSDPTLAGVVAVSPNIDPTLCVAALEQPRNWLYHWHFLS
                     SLKARMRRKAALFPDTWDLSPLGSMKTITQFDEAYTARDGGYRDVVDYYDRAGARHVL
                     HQIAVPTLIITAQDDPFIPATMFDTPAIRHNSNITLMMLRYGGHCGFFQKRCPQEDRF
                     WAENRILEWVGRMVTV"
     gene            complement(23959..26967)
                     /locus_tag="PK224_09940"
     CDS             complement(23959..26967)
                     /locus_tag="PK224_09940"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16098.1"
                     /translation="MKVLAFSALVNALAATGLGMFVYFRDPAAARNRIYGLYCLSIAF
                     WSVFYCGWQLTESKDLALSLLRLVMAGASLIPILYFHHTVTFLDITARHAKALKIGYG
                     LAALFLLADTTPWFVAGVHPAMSFSFWPVPGLFFHPFLLYFLWYVVYATSLVGVALRD
                     ANGIRRHQYAYMLAASIIGYAGGATNFPLWYGVPLLPYGTVLITMYTALMAYTIVRYR
                     LMNISVVLNKGLGYAIVLAIIVVATSIGAVLSNRATGHSTPPLLAGTLFLICALWVLS
                     NNPRSTSNTIFSAVCGAACLWLFGCFMLFSASREDEALFWVQVIYTGVVFLPALTYHF
                     AQSLSGGAAHDRLIVWNYAVGALFWSLLFTPYLVDGQYVYYWGRYPKAGVLHPWLVAY
                     VVAGGGLTVYRLYQGYRLHLSSSPLLGAQLKYTVVALALGFLASLDFLQNYGVGFYPV
                     GYLLAGLSVTVVAYAIARYELMDVSFVPSRPKVMLSIKLMGLIPAYMMILLVIRIFTG
                     TFHYLLAGVLFGLFVIVSSGLANLQKGVERAIGQTLFRERYDAYDTLVQFSKSLVAIL
                     ELKSLTKEIVQTLARVMNIKTASVYVLDKEQGIYSLTASYGFVTREAVVPPIKMEGEF
                     PKTLLRTETALVREEIEYDKADTEHLRLLDTLKALESEVCIPLISKDRLVGFCNLGRR
                     ANHGMYSTEELALLKTLAQHAAIAIDNALLYEDLRRSQLLMRRTDRLRSLETMAGGFA
                     HEIRNPLTSIKTFVQLAPERRDDVEFMEQFSQVVCEDVERIERLVHEILDYARYMTPK
                     LTQESLNDVVSSCLYFIEVKASSKAITIQKELASDLPYVKLDRQQIKQVLLNLFINAM
                     EAIGGDQGGRLSVRTRKLVKPVNEPWVQIEVEDSGPGIDPQDLDHIFDPFYTTKHESG
                     EREGTGLGLTIAHQIVQEHGGYVEVASEVGHGTRFMVNLPVNPPIAEWQAAQPAFDDG
                     RKLAGAFLARPHLGLDEQFGKPPAPVKAST"
     gene            complement(27001..29733)
                     /locus_tag="PK224_09945"
     CDS             complement(27001..29733)
                     /locus_tag="PK224_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB16099.1"
                     /translation="MSKHIAARNESVSVIGLLGQESRRQSSVPDRKKTSDAWSLSVAE
                     RRASRRLESRISTLLHFQDHTWNGQTKSLSLGGLSMEFSEDIPAMLNQQILLSVTPGA
                     TRPESVGVVCGIRVSESVLGSGQTKRGVTLAVQFARLSPGDEQVVAMWMSEGRARSKG
                     LHLVAALIAQESEDALIDTESRQPVVAQAARARLARVPERPRQERRRQQRITVGLSTE
                     VSLRLRAGDRILSGALTTDLTSNGACLCLSAEEDLLGSELELKWVSSTVAQRGSSVST
                     PSVGCSVVGEVVWTRPGEAGAPSGYRQVGGRTVLAGVRFLPVAKEAEDIIEHLLGQQQ
                     GEGVVDRAEGPSVISEFSECVRPSGHRIALCHDRPRTDLSDDAPIVVLAPGYGESKRD
                     YVPLAYYLAGNGFHVVRFDNVNHVGESDGLVTQFRLEDMETDLETVLDYVAAQWPGRS
                     IGLVATSLAGRVALKVAGRVPHLGLLAMINGIMDVQHTLQAVHQEDLIGEHLAGVRKG
                     VVNILGLTIDADRWLEHAVQGGYADLHTTQQDAEHLHAPVVLFHAEQDAWVDPASIEA
                     VGAAMGANLRHSYVVPGALHRLQESPRKARTVYRQIALCCQQELWPTRSMARMVEPSH
                     REIGVQNRAERERSKKRRPVGKSDHVAFWQEYLQNFQTIPNVADFWRLMDHVYRLMGD
                     CHHGERILDAGCGNGNLGVFLQLNQAFRQRYARRGDFRSPDYVGVDFVPTALAQAMTN
                     FEQVGAALQGQFYEGLRAYVPMSMRVCRADLEAPLPFPDHSFDRVVCNLVIGYVRDPL
                     FTLREYLRVLAPNGRLVISNLKPYADLSAIYRSFVETAQTPDQIEEGRRLLDNSGRIK
                     EREGEGIFHFHYQAELESLLRAAGVTHPRVYSTFGNQALIAVADKGLANLTVAA"
     gene            30431..30829
                     /locus_tag="PK224_09950"
     CDS             30431..30829
                     /locus_tag="PK224_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16100.1"
                     /translation="MRLPHIRAHSGMLIPVLAGIMLGCHSTPPITEGYPHQRALQGKS
                     KEQVLACAGTPLQEREDGAVTMLRYYKEAPLLEESMVSSKSSRPTVHHGCWATVVIQH
                     QRVDQVQYRFVPSSVDASNDCEEIFANCPE"
     gene            30826..31746
                     /locus_tag="PK224_09955"
     CDS             30826..31746
                     /locus_tag="PK224_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16101.1"
                     /translation="MTARRHRSVGPRWLLFFMGLLSAMMPWTEPALGLEKYGRPLPSM
                     DEPEHEAREEEETLFGGYLLTGAFVKNPTFAARPDNTGLVGMRHMLHLETDLYKQYLT
                     FYTDQNFFSDRTDGWIKLTEWDGTFAFTGVLDRFNWRLQYERDAPLDRRGLKQAYADA
                     LVTARFQAIQDSTWWRRTFPNQNLTAYAGAGWLFHNSQYFARPNNTGRALFRYVAHAD
                     LDVYKNKVVLYGDMNLFTDREASNTLNPSELDWIIGIAVRWKDTELAFYREEDRPLDQ
                     SGLVQKYYAVQLRFAFDVSKGALERMGLKR"
     gene            complement(31959..33449)
                     /locus_tag="PK224_09960"
     CDS             complement(31959..33449)
                     /locus_tag="PK224_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HRB16102.1"
                     /translation="MLNRIFGLETEYGLLVNQDRPDHSPSWVAQRIRDHIFHVERRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYIDMGHLEYASPECTTLADLVASDRAGDRIIQDA
                     VRALGLDETVSLIKNNIDHETDATFGSHENYLVTRQFPFSRRGLGPLVTFLVTRQVFT
                     GAGRIGCASDPNEWVQVGGLILHRPGLRDAQDRSIVPFQISQRADYIVNDFFEWVQHN
                     RAIVNTRDEPLADPNQYRRIHLLCGDSNMAEYATALKMGTTGLVLQLIEAGQAPRGLG
                     IHEPVEALQDISRDPDRRWIVRLESGQSMSAIDIQEQFLAAAQRHCKGQDEETDWVLE
                     QWESVLTDLRGGYEKLVGRIDWASKLWLLETYREAEQCAWDDPMLKSLDLEYHNLHPE
                     RGLCYGLEEEGRGPRLTTDKIVHLAQDHPPRNTRAFGRGELVRHLLASGGGGIVSDAP
                     LDFEEQMACEYIINWSNFKLRGTAPFFMADPFKTYVQDVRSHLAAR"
     gene            complement(33451..34290)
                     /locus_tag="PK224_09965"
     CDS             complement(33451..34290)
                     /locus_tag="PK224_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16103.1"
                     /translation="MYEEPYRWIEAVGNRRQYLDEQFAKGSPVVALAYADGILMLTVS
                     RGTPKLYEIYDRIALGGMGHPADLEKLRFSLLEMAHVEGFNRSPSDVTGARLMKYGLA
                     PTIKQAFEEIYKAPFIAKILLAELGVKPERNLFLSINYDGNFEESQGWAALGATASVQ
                     GRMRRYVESQPAFAQASLAQAVELALCTWAVGSLAQAASESTAAESSSTQASEEKTEG
                     DGSSLPDKAALLAHVRQTVAEKSLECVLLERRGPGSAKYRALRPEELGRLLPSAVSAP
                     ATT"
     gene            complement(34337..35110)
                     /locus_tag="PK224_09970"
     CDS             complement(34337..35110)
                     /locus_tag="PK224_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HRB16104.1"
                     /translation="MGMQGDFFQLLKEQGYQFGNPVAAAAGMDVPTATTILALKYRDG
                     VLVAGDRRATAGNIVMYDRTDKVLEIDRHSVMAIAGVPATAYEMVRVLEHSFKYYRRT
                     QLQELSFEGKLRAVSKLLKENVPAALAGTGAVVPVFAGYDHEQESAKIYFYDILGAEF
                     EAVEYAVSGSGSPTIRGILHYVNTWGPQPLATLPEEQATIQALRLLSSAAEFDSATGG
                     VNRELNLYPVVKLIKASGVQTIPDADLKRLHESEVLRGR"
     gene            complement(35215..35409)
                     /locus_tag="PK224_09975"
     CDS             complement(35215..35409)
                     /locus_tag="PK224_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HRB16105.1"
                     /translation="MRYMLMPERREAPGDPMPKPSGPSEEGGGPRRPETGSPDKDNLL
                     KRMRKVDPKQAERYRQRTGQ"
     gene            complement(35412..36932)
                     /locus_tag="PK224_09980"
     CDS             complement(35412..36932)
                     /locus_tag="PK224_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HRB16106.1"
                     /translation="MHLFGIETEYGITREDLDAVDPVEESMELVRAHLPGKFERRWDY
                     RGEDPHEDARGFRVSGLQQDKEEDDFVKVDAHRPFSFHEMKSDLVLPNGARFYNDHTH
                     PEYSTPECRTLKDLLAHDRAGERIVQRAADRRNQQLGGAHVQLYKNNTDFHGHSYGCH
                     DNYLVSRSLPFAQLVSGLLPFLVSRQLVAGAGKVGMEAQESGFVPGPFQISQRADFME
                     AELGVDTMHNRPILNTRDEPHADRTKYRRLHLILGDANMCEYATALKVGTTRLVLDML
                     QRGEAPSLELEQPVAAIKQLSRDPDLKTTVRLKDGRTISGLAIQEEYWNVASRCCAGS
                     DPDADWVLREWQETLCALRQDRTQLVGKIDWVTKQWLLETFMREERLAWEDPWLASLD
                     LEYHNVNPDRGLFLGLEAEGKTWRMTSERDIAQALVAGPSDTRGGLRGLCVRRFPEQI
                     TGMQWERVQFSGGLRARTLEMGDLFEPDAVQACAALLEAASSPADALAAWSRRKDG"
     gene            complement(36941..38686)
                     /locus_tag="PK224_09985"
     CDS             complement(36941..38686)
                     /locus_tag="PK224_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB16107.1"
                     /translation="MSDHRNPDQPESGGQQPPRREASASSDPIELIEECLAAFPDGDP
                     RQKLLYKLRHVITAQSVAQDRRDTELKKLNEVVAKLTAPANRVGLLLEVPAEAVARIV
                     VGGAEYYANIDPRLPVEDLKIGTQILVNEAYTVIKALGYDRNGPVLKVAEVLPDGRIR
                     FEQDMGRQALILQRSSDLLGADLKAGDEVRIEPTHRIAIEKFENRQARTHLLDEVPSV
                     TWAQIGGQHQAIEAIRKAIEYPLLHADTFTKYQFTQPKGFLLYGPPGCGKTLIGQAAA
                     ASLAQLVRESQGQAAADGTSKNPPVTSGAFLHIKGPEILNMWLGESERIVRDLFAKAR
                     ARRKEGALPFIFIDEAESVLGTRRSMRSFNINNTLVPMFCAEMDGIESLHDVVIILAS
                     NRPDLIDPAVLRPGRIDRKIKVARPSREAAVEILAVYLTPSLPLDRELLEQSGQDHEA
                     ARRAVIEQVVGSLFTRTDQNRVLSIRLRNGQNKVLYRGDLVSGAILSSIVQRAKEKAI
                     ERAVAGAPAGDGIRAQDLLDAVHEEYREGEMLPPDDAAEEWLKLLDHHPEQVVGVSSF
                     RRGRPTEERLVNQII"
     gene            complement(38784..39905)
                     /locus_tag="PK224_09990"
     CDS             complement(38784..39905)
                     /locus_tag="PK224_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRB16108.1"
                     /translation="MRLSGAIRKRWRGKRLRVGLVAALALSSPAWLQAAEGTKGLLIA
                     QSAAPTELSPEEQATIAVFERATRSVVFIANTAMQRDPWSFNLFEVPQGSGTGFVWSR
                     QGHIVTNYHVIYGADAITVTLADRTEYKAKVVGADPDHDLAVLQIQAPETALQPVIIG
                     NSQSLRVGQKVLAIGNPFGLDHTLTTGVVSALGRTIKSMSNRTIEGVIQTDAAINPGN
                     SGGPLLDSGGRLIGVNTQIMSPSGAFAGIGFAVPVDTVSRIVPELIKHGKLIRPGLGI
                     SLVPDAMARRWGVKGVIIGKVGRGSIAERIGLHGARETVGGRIELGDIIVAVDGQPVE
                     TIDDLMDLMEKHKVGDEVTIDYVRGKRRLQVMAPLQAVN"
     gene            complement(39907..40329)
                     /locus_tag="PK224_09995"
     CDS             complement(39907..40329)
                     /locus_tag="PK224_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRB16109.1"
                     /translation="MPVRSMSMQVKMQGDCRVENITGQVQAALSHTQLHAGILTIFIK
                     HTTASVLIIEDEPGIRADTRALWERLIPADPGWQHNVRNAGEDNGHSHLRGQLQGQSL
                     TIPFNDGVMTLGTWQQIVVLDFDTRARTRDLVLQVIGE"
     gene            complement(40319..41206)
                     /locus_tag="PK224_10000"
     CDS             complement(40319..41206)
                     /locus_tag="PK224_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16110.1"
                     /translation="MATTRMTSPPLLVPRTLVSQLLHLYDYPDPRRPGRLIKGYDCPH
                     ALRTARMCAAVAQRMGHQPARIKQYQIACILHDLGRAGLDRRLFGRIWSWARAQGIPT
                     RPREWRVIHPETRYGRETEAFVSRYRGAMEAAGIELNPWACEQVEMRLGYARRLAARL
                     RAVKPRLRELAVTWSPWMSRIMLYYYYPEKLKGAQPWVRQLAEILVACEQFEAYSNQR
                     RGRDYYVRQREDVSEAFAYLDALQGEGIISRPVVQALRELAAEGVFDRVLEEARGRSL
                     SSRERTFLRRAGQESLHAG"
     gene            41365..41988
                     /locus_tag="PK224_10005"
     CDS             41365..41988
                     /locus_tag="PK224_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16111.1"
                     /translation="MAKTRSMHIVVGSACLLLAQAFTPLLSAAAELIDLSGKAAAFVT
                     LRSRDNFSSEYRYDVTVRNNSADLFIADSLIIVLDRITNIGGQDRENLTGESLLNRME
                     IIGQDGTTEDGRPFFRVPQGSGPDLTPNSESLPVTVRIRNRDYVAVFTPSFRVMGQKR
                     DAPKQKSPAAAAAAAAAPNKATTDKLIQLLIKKGLITPQEAQSLSQP"
     gene            41985..42434
                     /locus_tag="PK224_10010"
     CDS             41985..42434
                     /locus_tag="PK224_10010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRB16112.1"
                     /translation="MSSQSCKACVGTWPDRSHFIADCGLTRAYLHDDQFFPGWTVLVL
                     KRHATELFHLSRDERSRLMEEVSGVAALLAQEWQAVKVNYELLGNQLPHIHWHLIPRL
                     PRDPAPLEPVWRIAHEPVRLEPDALASEIDRLRKIWPFHPNQSPVNP"
     gene            42453..43115
                     /locus_tag="PK224_10015"
     CDS             42453..43115
                     /locus_tag="PK224_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16113.1"
                     /translation="MANQPPPPSANRPRTFLRALLWSLPLHVLVSIAVVGGAAAYYSN
                     TVSSHGTWAAVGSWSLTALYVVGALVGGALAGVLSAAQRTVERLELTLRDWLHALPAM
                     QSASTSVGRSLATARQEYETMVDRCVTQTAGRLRLPRWLERLIRAALQGVVVDRFMTS
                     CTDRGLNLVAPQEFRNWLLAEGVSLGFMPIQDQLSWWRSLILGLLGLLVAIVLALAFL
                     NN"
     gene            43229..44503
                     /locus_tag="PK224_10020"
     CDS             43229..44503
                     /locus_tag="PK224_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16114.1"
                     /translation="MFLHHATKGGWPLILALALLIHPAWARAQEDPSAIPAPEPIREE
                     PLPPESVAQPALPTPIPAPVPLSLLETLTQTRQALHLEPDAQEPRLTLGRTLFQLGDT
                     DGAIDEYRTALRFHPTVAQAHLDLGTALMAKQDWRSAMTELQEAVRLDPTLVQAHYSM
                     GTIHYTRGNVQSAIKAYQEALRLKADFAEAHYRLGLVLKVAGRDKDAAQELEAAALAG
                     LAKAQYFLGNAYRSGQGVEKSQIMAITWWARAFEQGLPEAAQALTQLRRLAAVKGNLQ
                     TKQSKAAAEAFKNYCDQLWLDFPDLDRGDQATETVGTTLLKQGRTGEALPVLLREAYA
                     LNDISHAALVQLYEQGLDGQLPPHGQWILSYLESTAADGAIPSRTALARIYAKGLGLA
                     PDLAKAKSYLKGMPREEVKRILDEITPETPKP"
     gene            44685..47390
                     /locus_tag="PK224_10025"
     CDS             44685..47390
                     /locus_tag="PK224_10025"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB16115.1"
                     /translation="MQIATRLFVRNVWLQAVVMALASLALTEAMWAPAPVTYHALEWA
                     PYDTWMRLRSQPAPDSHLLLVMRDQASEQQFGAGLWDRSLPARLITGLHDAGAAAIGL
                     DIPLDFPSPPNLGGAVSDALLMEAVTSAGTVSYPAFPTAPIDQDRVGFAVPAQGLTPG
                     RSTLQPLLDLDRIARRAGLFYGNGSDELPALGFSLATTFWQIPLRQVERRSGQVRVQN
                     ARWPGGTTGSLTIPLDRQGRLLLNFAGRQVPTAFPGTTVLELSRLLDRKDNEALGTLV
                     NGKVVVLLIQPHSQPERTLPSGDDASDMLLQAHLLHTLLTQDWIHELSPLQRLLVTFA
                     LCLWVAWLVLRWTDWKGLLLGAGSLLLYLTFGLVSLTTAHWVLPFVIPVTAAVTVLVA
                     TSLLGQVVATRRLALLEQDMLQVQQNLGAVREALIFRETAVESLEEDLESARAGMSHS
                     ASREAESTRLAADLQLKIADAQAQEEATRQRMNELERQLQSLRAATISSVPLGNVEQE
                     TLRRECEQVGIVTGAATILTMFRDLKKGARSTVTVLITGEPGTGKELFARAVHRLSPR
                     APKPFIAVNMAAISPELFESELFGHVRGSFTGALMDRKGYFELAHHGTIFLDEIGDLR
                     LDHQSKLLRVLQDRTFYRVGATTPTTVDVRIVAATNKDLQRGVSEGWFREDLYFRLKG
                     LVLHLPPLRDRPDDIPPLAEAFLREAANQPQHANMRLSEEALATLQQQAWRGNVRELR
                     QCLEQAIALSDGPIITTADLRLSGQPSPTVVVTGAEPLLPDPAGDIAVLNQLRQHRFD
                     MQATAKALGWDRSTVTQRLKGLCFQALVESGRDQTKAASALAGDPSLLRAVELKLMDY
                     YGHLMETIEPFTTAEDALLDCKRRFKNLPERHFKSVEVLVRQHFG"
     gene            47612..48619
                     /locus_tag="PK224_10030"
     CDS             47612..48619
                     /locus_tag="PK224_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB16116.1"
                     /translation="MTADAAHRLACEGTALLWRGDFQNARQLLNAMARRADRHPPKPG
                     TSPAESFHFHRQTQSRRARILGMLLVPLEEDYAIPLRRAPDLRLACTEAYGPCEGQAL
                     VSLRELLGLVGAHEWRKNGVVVPALGDRIHPHYGVFSPVRSEYVDLVADAPLPSNALA
                     FDIGTGTGVLAAVLARRGVSHIVATDQDPRALACARENLARLELTDRVEVVQADLFPE
                     GKAPLVLCNPPWVPARPTSPIEQAIYDPESRMLSGFLSGLAAHLEPGGEGWLLLSDLA
                     EHLELRTRAELLAAFDQAGLVVLGKTDIRPHHPRASDQADPLRAARAAEITSLWRLGM
                     R"
     gene            complement(48741..49976)
                     /locus_tag="PK224_10035"
     CDS             complement(48741..49976)
                     /locus_tag="PK224_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16117.1"
                     /translation="MLKSHKKTILQALGRNPIHPFPARMAPGIALEALGKSETPLRVL
                     DPMAGSGTVLAVARAKGHRAFGMDLDPLAVLLAGVWTRTVDGMRVKDKAIEVLDRAKV
                     AFKPLSSGQAYPSGSDDETRKFIRYWFDDYARRQLTSLSMTICQVHDDATRDVLWCGF
                     SRLIITKSAGASLAMDLSHSRPHREFTHAPVKPFNRFISAVNTVVANCPQLGSVAVGP
                     ATVVKRGDARKLDNEDGSIDLVLTSPPYLNAIDYMRCSKFSLVWMGHNVSELRQIRGE
                     SVGAQASSKEALESPCVKSLIRELGLKPALSNHDHALLARYVWDMSQALAEVSRVLRP
                     GGRAVYVVGDSTSRGTFIPNSSIVTAVAQEHGLSLVSRQSRALPANRRYLPPPKRGIS
                     KATMDIRMRREIVLAFKKP"
     gene            complement(50065..52737)
                     /locus_tag="PK224_10040"
     CDS             complement(50065..52737)
                     /locus_tag="PK224_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16118.1"
                     /translation="MDVKKSRQAESSFPETIGCATGDISVELSTRFLEHFSEQLYSSP
                     QKAFEELISNGWDAGADCVDVRISTDLNDPKATMCVLDNGSSMDEEGLRQLWHIAFSP
                     KKDKPFQHGRPIIGKFGIGKLATYVLASKLTYICKASDGIIRRVTMDYGDIDQQKGAV
                     TDRLISDLTIKLYEVAELEVEAALASVYDGQAILKLIRGGIPQPDGKLSDDEFGSPKT
                     ELTRPSANTWTLVVLSGLKPAGRELKIGVLRRMLESALPFGSEMAICLNGELLASSKI
                     NTPSMAEWIIGPELDIDSIEIDERYGVEGQSQNHSADSDEASDSPAKLTKVPIKSGTS
                     PVPHVEIPGIGIITGRVWLFVDRISGGKSEARGTSNGFYVNVLGRLVNQNDPSFGEEN
                     LSHAAWARFRMTARADGLNELLTTDREKFRNLRALKIFRAFLRRVFNKARTHYDSDMN
                     ATIPDGGDVLVRSLGVLSLNPLRNVVSETLRTQPPLPGLFDETGITDREGKRKSWREN
                     TAENIKTALGEVKYENSGDDSFVKFRISDSTIVVNKEHPFVAEHTRTKAEKELLRTMA
                     MVSLLSDVYALDLGISPSVLNNIREYRDKLLRYRAMQRRQSGIYIAKLLLQTQHDSGN
                     SKRLEAVISDALRYIGFEVQDLAKPGEPEGIARAYPIPTSSSPTQENPNPPLYSFSFD
                     AKSSKHETAATGNIKLDGIVEHRKRYEADYALVIAPGFSEGALAVRCEQQQVTPIKAS
                     DLGKLLEYTVEHGAIPLTKFREVFRLYDPEKVSQWVADLETWLNEKRPLTIDIFLKAL
                     KALKGKIPDALPAGTIALICRDSLHAASVKDEDVIAIAKGLSISSPDIVGVDGDKIII
                     NASAEKVAAVIEAQLERLHSDDIIMPEGPGEAGQ"
BASE COUNT        11410 a        16651 c        14200 g        11085 t
ORIGIN      
        1 tgctccatcg ccagcagaat tttttcttca atctcgcccc tcttcttatt ggcaagttcc
       61 acttcgaaga gatgcgcctg atattcctga ttcgttttta actgggcggc gcggtccttc
      121 atcttaccga tacggtcttc gtgcgcctca agatccttct cgtgacccct gcgatcctta
      181 acgagcgatt cgacggaagc ggacgcctcc cgaaacaacc gcttcgcctc gcgaagcggc
      241 gcctcatttt cttccagccg ctcggggatt tttcgccgct gctccttgag atcggcgatc
      301 cgaaggtcga gtttttgcaa ttcaatgagg ggggaaagct gtaggttcaa cgggctcctt
      361 gtgggttatg cgataccgat gtcgaccgga caccgttcgc acggaattca tcgtgaggca
      421 ctcaggttgg tgggcccact aggacttgaa cctaggacca gctgattatg agtcagccgc
      481 tctaaccacc tgagctatgg gcccagccct gcgagccagt acactgaaca tagtccgcct
      541 gatgcgatgg cctatgccaa ccggccctgg gagatgcaca cgccacccac acctggttcg
      601 agcgggctcg attctaggct gccgcttaag cagagtcaaa ccacccgtgg ctgggcgcct
      661 agaggctctc cacaaaactg cgcaacttct tgctgcgact cggatgtcgg agcttgcgca
      721 aggctttcgc ttcgatctgc cggatgcgct cacgtgtcac ttcaaaatcc tgccccactt
      781 cctcaagagt atgatcggtg gcctcgccga tcccgaaccg cttgcgcaag accttttcct
      841 ctcgaggcgt gagggtttcc agcgcgccgt tgatctggcg ttgcaagtca taccgaatgg
      901 ccgcttccaa cggcgagacc gccttcttgt cctcgataaa gtcgcccaag tgactgtctt
      961 cttcctcgcc gatcggggtt tcgagggaaa tcggttcgcg cgcaattttc aggatttttc
     1021 ggaccttgtc gagcggcaag tccatgcgct cggcgatctc ttccggggtc ggttcgcgac
     1081 cgagcttctg caccagatgg cgtgaggtcc ggatcagctt gttgatcgtt tcgatcatat
     1141 ggaccggaat gcggatcgtc cgggcttggt cggcaatggc acgagtgatc gcctgccgga
     1201 tccaccaggt ggcgtaggtg ctgaatttgt acccgcgctt atactcgaac ttgtcgaccg
     1261 ccttcatgag gccgatgttc ccctcttgga tcagatctag aaactgcaga ccccggttcg
     1321 tgtacttctt ggcgatactc acgaccaacc gcaggttggc ttcgacaagc tccgcctttc
     1381 ctcgcttcac tttttcttcg gccacatcga gatgcttgac cgcgtcctta atttcttcgg
     1441 cagaaatcag cgcctcttcc gtctcaagtt ggcgaatccg gcccttggct gcctgatagt
     1501 gcttcttgat gtcctgcagc acctcttcag agaggctggt ccgccgtttg accgccagaa
     1561 gatccttgtg tgtccggcac aaccgccgca agagctccgc accggcttca ccgccgacgc
     1621 ccaaccggcg ttgacagctc gtgacttcac gttctgcttg gcgaaacagc agattgaggt
     1681 cccgaacccg ctgcgtcatc cggtctttca acacaccgtg cagattgacc gactccatct
     1741 tgtcgacgac ctgcccgcgc accgtatcga tctgtttgcg cagtttcttc tgcttctcag
     1801 gatcggcgcc cacatggcgg gccttgtcgt acaagtcttt cagcgaggcc gagaccttac
     1861 ggaccgcatt gagagaatcc agcgttttga cccgcagctc ttcgtagtcc ttctcgacgg
     1921 cttcttcttc aaagtcttct tccgtctcga ccaccggcac gatctcccgc acatcgatct
     1981 tgccgttctt cagctgatcc cgcagattga gcacgaactc cagagtcatc gggaggccgt
     2041 aaacgaccgt ggcgatatct ttcttgccct cttcgatccg cttggcaatc tcgatttcgc
     2101 cctcgcggct cagcaacgca acgcttccca tctctttcaa gtagagcctg acgggatcgt
     2161 cggttcggct gagggcccca ggtgtcagat cgatctcttt ttcattttcc tcggacgcct
     2221 cggcttcttc gctttcctcg atggcctctt cgccttcagc ggccttcggg gcgcgttcgg
     2281 tctcagcaga ctccacaatc tcaatgtcca tttccccgaa catcgtcatg atcgtgctga
     2341 actggtccga tgacacgaca tcggccggca acgtgctgtt gagatcgtcg taggtgagaa
     2401 agcccttctc tttgccgaga ttgatgagct tcttcacctc gccgagtaat tcttgtttcg
     2461 gcatacctac tccttcaccg cagacgtcag cgcagccggc atcacgccgg ccttccgaat
     2521 ccgcatctcg ttaatcaatc cgttcaggcg gtgcacatcc gcttcacgcc gctcccgttc
     2581 ggcggccttc aactcctgaa tcagggcccc catggtccgc tcacggcctc tccgctccag
     2641 cgtttccaaa catccggcaa tatgtgccgg gacatcatca taatgctgct ccagcatcga
     2701 caactcagac actaatgagc cacagttctc gtcattgatc aaggcatcca gcaggccccg
     2761 gacggacacc cgcccatcct gctcaagatg ctgcatcgcg cattcaatca gtcggcgata
     2821 ggccggtgcg gaaaaggcgt ccggcgacaa cttccgaaga tcctcggccg acagacttcc
     2881 ctgcaccaag agatgtgcca gatcacgctc ctccggattg cccttcggtt tcgacgccgc
     2941 cgcagcgggc agggccgtgg gccgggaaga aggccggcgt gtctcctcgg atgccagcgt
     3001 cggatagcga tcaatcaatc gttgctggct gagtcccaac cgttccgcaa ctaaacgaat
     3061 ccgttcctct cgctcgatgg gatgggcgct tttctgcaga atccgtaaca ccgcatccac
     3121 agcgcggatc cggtcttcca cggtcccgga ctcagccgtc cgcagactat gctccacggc
     3181 aaaatccagc agactcgggg ccgcttcatg taaagccgta aacccttcgg cgccgtattt
     3241 cctcacgtac gtatcgggat catctccctc cggaagcgag acaaccttga cgctcaaccc
     3301 gctattaacg aataaatcca acgtccgcag tgcagcccgt accccggcct gatcgggatc
     3361 gaacagcagc acaacattgg aggcaaagcg cctgatcacg gtgatgtgtt cggcggtcag
     3421 ggctgtgccc aatgtggcgg ccacatggcg gatgccggcc tggtgcaacg ccacggcatc
     3481 gaaataccct tccacaatga tcaatgtgtg ttgctgcccc gccgcctcac gagctgcttc
     3541 cagggcgaac aaggtctgcc ccttcttaaa gagcggcgtc tcgggggagt tcaaatattt
     3601 gggcatcccc tcgccgagaa tacgtccgcc gaacgccacg acccgcttgc gcagatccat
     3661 gatcggaaac atgacccggg cgcggaatcg atcgtagtgg cctgacacgg cggctttctg
     3721 ttgtccgctc tgatctcgcg gaatggacag gcccgccgca accagatccg caggggcgaa
     3781 gccctctttc aacatagctt tggtcaatcc atcccactcc ggcagggcat agccgatttg
     3841 aaactgcgcc attgtgttgt gctgaatgcc gcgcccgtcg agataagcac gggcaggagc
     3901 accggcggtg ttatccagta acatgcgctg ataataggcc ccggccgcag cattgagccg
     3961 ctccagccga cccagctgcg tgcgcgcctc gctggaatat ccaccggccg actccggaac
     4021 atccacccct accttgcggc ccagatcacg aacaatctca ggaaagccgg ccccggtcaa
     4081 tttcatcaaa aacgtataca cattgccgcc ggcgccgcag ccgaagcagt gaaaaatctg
     4141 tcgcgaagag ctgaccgtaa aggagggaga cttttcttga tggaacggac agagcccctt
     4201 aagattctgg ccggctcggg tcaatgccac gtgttgaccc acgatctccg cgatgtccac
     4261 tcggtctctg atttggttga tgacgtcgtc cgaaatcagg ccttggccca cggcggtcct
     4321 cgcttgtgcc cgcagggatc caaccaggct gcagccggct ggagtgcatt aagcgcgatt
     4381 gaaagttgac cgttcaccct aacaaacggt ctgcataagt gtcaatcgag gagaggagcg
     4441 gattatcccg gaggccaggc catgtgcctc ccgccgagca catgaagatg gagatgaaac
     4501 acggtctgcc cgctctccgc gcccgtattt gtcacaatcc gatagccgga ttcagccagg
     4561 tttttcatgc ctgcaatttt tgagcaggtt aacaggagat gccctaggag agcctggtct
     4621 ccctctcgac agtcctgcac agcggccaca tggcgtttcg gaatcaccag gatatggacc
     4681 ggagcctggg cattgatgtc atcgaatgcc agaacctgat catcctggta cacaattttg
     4741 gccggaatac cgccttcaac aatgcgacaa aaaatacagt tatccacgcg atcccccctc
     4801 aggcgaaggc cggatcccgg actttccgaa gcgggtcccg agttcacggt acacctcgtt
     4861 cagcgtcaca tcgtggtacc ccagcaccag caaggtgtga aagagcagat cggctgtctc
     4921 gtagatgatc tcttcccgct tttgattctt tgcggcaata atcacttccc ccgcctcttc
     4981 agcaaccttc tttagaatgc gatcggctcc gccctgcaat aatttcgaca cataggagtc
     5041 gggctgcgga ctggccttac gcgtcagaat ggtctgatac acccgatcca agatcccccc
     5101 accggcatcc tgggtcttct cctcactggc ctgcccctct tccgtcatcc gggcgaaaaa
     5161 gcaggcacgc tcaccggtat ggcaggtcgg cccgacaggt tcggccttca cgagaatcgt
     5221 gtcacgatca caatcgacaa agagatcctt caccttgagg aaatgcccgg acgtctcgcc
     5281 cttttcccac aacttccggc gagaccggct ccaaaagtga acggatctcg tctgaagcgt
     5341 cctggcaatg gcctcctgat tcatgtaccc gaccatcaac acggtgccat ccagccaatc
     5401 ttgaatcacg gcgggaatca acccttgtcc atcaaacgtc atggtccgac tctctttcga
     5461 catcacattc tgctccatct aggctggtcc cagtcgcacc ggaaccccgc ggtctcgcag
     5521 atatgccttg gcttgctgaa tggtatgagt tcgatagtga aagatcgagg cggccaggac
     5581 ggcgtccgct ttccccttca ccaacccgtc gtagaggtgc tccagggtcc ccaccccgcc
     5641 cgaggcaatc actggaatcg agagacgttc cgaaaccgcc gcagtcaacg ccagatcata
     5701 cccgttctgg cgaccatcct gatccatgct ggtcaagagg atctctccgg ccccataccc
     5761 ttccatccgt tgcgcccact cgacggcgtc caggccggtt ggttttcttc caccgtgggt
     5821 gaacacttcc cagcgacccg cctgaccgga ctgtttggcg tcgatcgcca ccacgatgca
     5881 ctgcgtaccg aatcgctggg cagcctcgcg aacaaattcc ggctgctgca ccgccgtggt
     5941 gttgatgctg actttgtcgg ccccggcatt gagcaaagtc cgaatgtcat ccagcgtacg
     6001 caccccgcct ccgaccgtga gcggcataaa cacacgagcg gcggtttgct ccactacatc
     6061 gatgatggtc ttgcggtttt cgtgtgaggc ggtgatatcc aggaaacaca attcgtccgc
     6121 gccttcgcga tcatagatgg cagccacctc gaccggatca ccggcatccc gcaaattcac
     6181 gaagctcacg cctttgacga ctcgaccatc cttcacatcc aaacagggaa tgatgcgctt
     6241 agtcagcata gaggttcgtc aagcgtgact cgtgaagcga gccggtttta ttttcgtcca
     6301 gccgcatgca agatcctcaa cgttttccga tggctgcaac cgctgccgca taatccaatt
     6361 tcccgtcgta gagggccttg ccgacgatgg ccccctccac gcgaggacca agcgcatgca
     6421 ccgcctgcag atcttccaca cgcgtgatgc caccggacgc gatgacggga aacgacgaac
     6481 aatcaacgac ttctcgcaac gccgtcaggt tcggcccatt gagcattcca tcccgggcga
     6541 tatcggtgta aatgacggcc cccagttcaa ggccggcaag ttctttgagg agatcgatgg
     6601 ccttcgtttc ggacaccgcc gtccagccct tcaccgcgac cttcccgtct cgcgcgtcga
     6661 gtcccaacaa aatacgccga gggaactcct gacaggcctg cgccagaaac gttcgatcca
     6721 taagggcagc cgtgccgagc accacgcgcg ccacacccgc atggagatat cgccgcacgg
     6781 tctcaatggt ccggattcca cctcccacct gcaccttcac gctgactgtc ttcatgaccg
     6841 cttcgatctg gggaagattc ttcggctccc catcgacggc tccgttcaga tcgacgacat
     6901 gaataagatc ggcgccttgc tgctgccagc ggccggccac cgaaggcacg tcctctgagt
     6961 acaccgtttc agcggccatg tcgccctgcc gcaatcggac acagcggccg tctttgagat
     7021 caatcgccgg aattacacgc atgacaccac ctgtcgcaac acgttcattt tcccccgcct
     7081 gctgcgccgc catatggaaa cgcatctgcc aattcctccg ctccataggc ggccaactcc
     7141 tcggcagtga caaacacgcc atagcgctgg acaaagccta aaaagtcttc cgtcatcggc
     7201 cgctgctttt catagtagtt gccctcccac aggactatgc cgtcgggcgc taccagcttc
     7261 acctcgaatc ccacggcggc cggtgggtcc gcgccaagcc gactgccgac tcgttcctga
     7321 tagacaagca ccttgccgct gagtgccgca tcggctccga gtcgttgagc aatcttgtgg
     7381 gccggcatcg tttccgtcgc agtccccccg gaaacttccc gagcaaccct tgccgattcg
     7441 ttaggggaga gtagttgtaa cccggatcgg gccttgagtt ttgcccacat cagatcggtg
     7501 actttctcac ctgcggaggc cggtacgcga tgagtctggc gcacggcttg atcggtcgtc
     7561 ggagggaccc cgacagccat gtcggaccgt cttgcgccgg acgggacggg aaacgcgggc
     7621 ccagacgcct cgatcgcttg gggagtcgcc atagtttcaa acggaatgag ggcgattgtg
     7681 cggaccggat actttcccac ctgcgatgaa gctttggacg tgaccttcgt cccgctgcag
     7741 cccgccagaa gcagcaacgt catcggaatc aatagagccc gcacagaccg gtctctcacg
     7801 tccaggcccc gaagttcttg atcacttgaa gtccgacagc ttggcttttt tcaggatgaa
     7861 actggcaggc gacgacattg tctttccaca cggcggacgc aaaggaccgc ccgtactcgg
     7921 tcacggtggc gatcaccgaa gactcaaccg gctcaacgta gtaggaatgc acaaaataac
     7981 aataactgcc ggtcgcgagg cctgcaaacg gaggggcgga ttgcacgatt tgaatctcgt
     8041 tccaccccat atgtggcacc ttcagcggag gttccagcac aaatttcttc acccgaccag
     8101 gaatgatgcc gagacctgcg tgcaccccga actcttcact ctccgtgaac agcaattgca
     8161 gccccaggca gatacctaga aaaggttttc cggattgaac ggcggcgtga atcggcgcga
     8221 ttaattcgta ccgttccaga ttcgccatgc aatccccgaa ggcccccaca ccgggcaaca
     8281 ccacgtgact ggcatcagca atgacacgag cctcgcgcgt gaccaccgct tgatgcccca
     8341 tcgcctcaaa ggccttggag acactccgca aattacccat gccgtaatcg atgatggcaa
     8401 tcatatattg cgttcggaga cggcccgtgg ggacttgatg gacgacagac aggacggcag
     8461 acactgcctc ctatcgcgcc accggtttca cttagaggct tcccttcgtg gagaggactg
     8521 tccctgacaa gcgctcttcc aacgtggtgg cttgatccaa cgctcgggcc aacgccttga
     8581 agatagcttc catgatgtgg tgcggattgc gtccgtattg gagattcaca tgcaaattca
     8641 gcccaccgtg ggtcacaaag gcctgaaaaa aatcctcgaa caggccgaga tcgaaggtct
     8701 taatctttcg atccggcaaa ttgacgttgt acacgagata gggacgccca ctcagatcaa
     8761 ccgtcacctg cgcgagggtt tcatccaatg ggaccgaggc gaatccgaat cgcttgatcc
     8821 ccgccttttc acccaacgcc tggtgcagga ccttccccat gacaatcccg acatcctcta
     8881 cggtgtggtg ttcgtcgatt tcgatatcgc ccttggcctg aaccgtcagg tcgaagaaac
     8941 catgcttggc gagaagatcg agcatgtggt cgaagaaccg gatggaggtg tcgacagtcc
     9001 cctgcccggt gccgtccaga ctccagtcaa cgcggatatc cgtttccttc gtggcacggt
     9061 gaagcgaggc ctgcctggga gcgccgttct ttttcatgcg aacctgcttt gggctgattt
     9121 ggcatgcgca tcgaagccct cgatatgcgc cagtcggacg atatgatcct tggctttcgt
     9181 caactcttcc ttggtgtaat gcacgatgtt gctcaccttc acgtaatcgc tgaccgacaa
     9241 cggcgaaaag aagcgggccg tcgccccggt cggcaacacg tggttcggac cggcgacgta
     9301 atccgcgacc gcaggtggcg tgtagcggcc caggaacagg gccccggcgt gccgcacctg
     9361 ctccaaataa tcgaacggcc gatcaaccga gagggtcaag tgctcgggcg caatcgcatt
     9421 cgccacctca atggcttcat ccatggtagg aaccacgaat gccacaaaat gccgggcaat
     9481 cgacttcgcc gcaattttct ctcgttgcaa gcctttcaac tgcacctgaa tcaggcgcac
     9541 cacttctttg gccaacgacg cggaggtcgt cacgagatag acctgcgcat cttcgtcatg
     9601 ctccgcctcg cagagcagat ccgccgccac atgtgccggc ttggcgtcat catccgccac
     9661 cacgagcaat tcactgggac cggccaccat atcgataccg acggtgccat aaagtagtcg
     9721 cttggccgtg gcgacgaaaa tatttccggg acccacaatt ttatcgacct tggcgatggt
     9781 cttcgtcccg aacgccatcg cgccgactgc ctgcacaccg cccacacggt agatttcatc
     9841 gacaccggcg atatccgcgg ccaccaacag atagggattg atctcccctt ttggggtcgg
     9901 ggtacacatg acgatacgcc gaacaccggc gacttttgcg ggaatcgcgc acatcagcac
     9961 cgatgaggga tagacggcct tgccgcccgg cacgtacaca ccgacagcgt ccaggggcgt
    10021 gaccatttga ccgagcgtgg cgttttgttc ctgatacatc caggttttgg tgcgctgacg
    10081 ctcatggaat tgccgtacac gttccgcggc atagcgcaaa gagtctccct catccttgcg
    10141 aatgtggtag taggcctcct tgatctcttc gggggttaca cgcatgtgat cgagcttcaa
    10201 ggacagccga tcgaacttct tcgtgtaccg gaggactgca cgatctccgc cccgctccac
    10261 ggccttcaga atcgtgcgca cgctcttctc cacacctgcg ccttgagtgc gcgcgcgggt
    10321 cacgattttt cgcaacgcct tgtcatacgc ccgatcggta cttgccagaa tggtcatatc
    10381 atctgtctcg ccgcttcgac acccgccacg aactcatata cttacactta ggcgcgcgat
    10441 gctgccccgt tgctatcttc caccgccacc gcggcgcgca accgctcgat caacaccgtg
    10501 agaggttcat gtttcaattt gaggctggct cgattcacaa tcagcctggc ggtcgagtgc
    10561 gtgatcacct caacctctgc caggtcatgc gccttgagcg tgctgcctgt ttcaaccaaa
    10621 tcgacgattc gatcggctaa tccgacgatc ggcgcgagtt ctatagaccc gtacaatttc
    10681 acaatttcga ccggcacccc acgctgattg aaataccgct cggttacgtt ggggtacttc
    10741 gtcgcaaccc gaatcttcga cgaccaccgg tccggcgaag gctgcccctg cagcgcagcg
    10801 accgagattc tacacgctcc gaccttcaaa tccaacggct catagacgtc gctgccctgc
    10861 tccagtaaca catctttgcc caccaccccg acatccgccg ctccgtgctc aacataggtg
    10921 ggcacatcgg ttggcctgac gatcaagaag gtggcatcgt tggacgaaca ggcaaacacc
    10981 aacttacggc tctccgtcga caggccggga ctataatagc cggcttgctt cagcaggtgc
    11041 agcaccggtc ccaacaactt gcccttggac agcgcaatgg tcagcccccg cgacttcttg
    11101 ctgtgcgatt tcgtcgcccc cacggtcact tcccctttct ccgctctaca ctggcgccca
    11161 acgctctgaa cttctcttcg agacgctcgt agcctcgatc gaggtgatag acccgcgaga
    11221 cttccgtcgt cccctcggcg gcgagcccgg ccacgatcag tccggcgctg gcgcgaagat
    11281 ccgaggccat gaccggagca cctgttaacc gttctttgcc cgtaatgacc acccggtttc
    11341 cttcgactcg aatatccgct cccatacggc gcaactcctc cacatgcatg aaccggctct
    11401 cgaaaatcgt ttccgtgacg acgctggtcc cttcggctac cgtcatgagc gcaacaaact
    11461 gcgcctgcat atcagtggga aaaccagggt gcggactggt ccggacattc gtccccttca
    11521 agcgcccggt gaccttgagg cgaatccgtt ccttttcctc gatcaactcc acgccgctct
    11581 cccgcagttt catcaacaac ggctccatgt gctccgggcg acagcaatca atcaccacct
    11641 caccgccggt aatggcgccg gcgataagat acgtacccgc ctcaattcgg tcgggaatca
    11701 cggcatgatc cgcgccatgt aactccgtca caccctcaat cgtaatcatg tccgtgcccg
    11761 ccccggtgat gcgcgcgccg cgcttgatga gaaaggcggc cagatcggtg atttcaggtt
    11821 ctttcgccgc attttcgagc accgtagttc cctcggccag gacggctgcc atcatgaggt
    11881 tctcagtacc ggtcacgctg ggcgtatcga agtaaatacg agctcctctc aaacgacgtg
    11941 cggtcgcctt gatatagccg tgctcgattt cgacggtcgc gcccattttt tccaagcccg
    12001 cgaggtggaa gttgaccggg cgagacccga tcgcacaacc gcctggaagc gacaccttcg
    12061 cctcacctag cctggccacc aggggtccca gcactaagac ggaggcccgc atggttcgaa
    12121 caagatcgta cggcgcttcc gtggaagcaa tcgcctgcgc ctgcacaacc gtatgattca
    12181 cctcctgcgc gacggagatg cccagcatgc cgagcaactt ccccatcgtg agcacatcga
    12241 cgacccgagg catattcgaa agcacacact cgccgccgcc aagaatggtc gacgcgagaa
    12301 tcggcagcgc ggcgtttttc gccccgctgg tgcgcacttc ccctcgtaac ggcctgccac
    12361 cctggataat gatttggtcc atgagaatta agaagacgat ctgctcgttg ccgtttggat
    12421 gcttggttga ccgctctgag gattcctccc ctcttctttt ttctcgaaac agaccacgcg
    12481 gtcaatgcca ccttcgtccc gcagaacatc gtaggtgcga aaccatccgc tttcctcggc
    12541 ctcgcggcag accgccgccg cctgaccaag gcccacttcc agcagcagca caccatccga
    12601 cttcaaatag acgggagctt gctgcagaag ccgacgatgc acatccattc catcgggccc
    12661 gccggctaac gccaaatgcg gctcgaaaca tcgcacttcc ggttggagcg tcaccagatc
    12721 gaggtcgggg atatagggag gattcgacac aatgacgtca atttgactca cgaacccaca
    12781 ctcggccaac ggcgcaagga gatccccctg catacattca attcgtgcat cgagtccgtg
    12841 ttgttccata ttggcctgcg ccacagccaa tgcatcagcc gaggcatcga tcgccagcac
    12901 tcgcgcgtca ggcagggccg atgccacaga cacagccagg caaccggacc cggttcccac
    12961 atccaccact atcgcggcgg gattctgccc gcaccgacgg atcgcctctc gaaccaataa
    13021 gacactctcc ggccgtggaa taaggacagc ggatgtgaca cggaattccc gcccgcaaaa
    13081 ctcctgcgta cccagcagat actgcagcgg ctcacggttg gcccgccgtg cgacgagcgc
    13141 tagcaccctc gcatagtcca gcgccggcac cggacgctcg gccttcaccc gctgcatgag
    13201 cggcgagaga tgcagcacat gctccaacag ccaggcagcc tccaatgccg gctgctcgat
    13261 accggccgca tgcagactcc gttcagcctc agccagcatg gaacggatgg taactgcgga
    13321 agtggcggca atgccgagct gctccgtact catttcactt ccaaggaggc tacatgttca
    13381 tagtgcgcgc gtagcgcctg aacaatctct tccagatcac ccgccaagac ttgatcgagc
    13441 ttgtggaccg tcaggccgat gcgatgatcg gtcacacggt tctgagggaa gttgtaggtg
    13501 cggatctttt cgcttcgatc acccgttccc acctgcgatt tccggttttg tgcgatttcc
    13561 gcatcctgtt tttcacgctc agcttcgaca atgcgcgccc gcaaattccg catggctttg
    13621 gttcgattct tgagctgcga ccgctcatcc tggcagctga caaccacacc ggttggaatg
    13681 tgcgtaatgc ggaccgccga ataggtcgta ttgacactct gcccgcccgc acctgacgaa
    13741 caaaacgtgt cgatacgcaa atccttcgga tcgatatgca cctcgacttc atccacctcc
    13801 ggcatgaccg caaccgtcac cgtcgaggta tgaattcttc cactggcttc cgtggtcggt
    13861 acccgctgca cacggtgcac tccgctttcg taccggagac gcccgtacgc gcccttccct
    13921 tcgagtagag cgatcacctc tcgataacca ccaattccgg tctcagacgc atcgacgact
    13981 tctaacttga gccgattgcg ctcggcatat ttgagataca tccggaacag atctccggca
    14041 aacagggctg cctcatcccc acccgttcca gctcgaattt cgagaaacat attcttctcg
    14101 tcgcgcggat ccttcggagt gagaaagtct ttagcgcgat gctcgagatc cagcacctgc
    14161 tgctggagat ggtcactttc ttccgcaaac agctgcttca tgtccgcccc tgtcgaggcg
    14221 tcctggagca tggcagatac ttcgtccagc tgcttcttcg cctcgcagta ggcgacaaat
    14281 tgaagcgccg gctcttccaa ctctgtccgc tccttgttca gcttgtggat catccccggc
    14341 tgattaagga cagacggatc catgagctga ttggtcaact cttggaagcg cgcagccatg
    14401 ccttcccact tcttgatcag cccgtcttcc atgtcccgcc cattccggcc gtagcccgct
    14461 ttaccccaaa aaacaagaga ccctgcccca aaaggaagca gggtctccat gatgtcctga
    14521 ctgaggccta cttatctttc ttggcgtact tcttcttgaa ccgttcgaca cgcccttcgg
    14581 tatcgacgat tttctgagtt cccgtaaaga agggatgaca cccggcacaa atgtcgacct
    14641 tgatgtttcc gatggtggat cgcgtcttga acgtgttccc gcaggcacaa tgcacagtcg
    14701 cttcacggta gactggatga attccctttt gcatacgagc tcctctactg cccttgattc
    14761 gttgactcgc ccggtcctgc tcgccagggt gcacgacttt acactggagt gccccacact
    14821 ggcaagcgcg acacctaacg attcatggat tgcaaaaatt cctgattggt tttggtgccg
    14881 ccgatcttgt ccattaagaa ctccatagct tccatcgtcc ccaacgggct caacaccttc
    14941 cgcaggatcc acatcttgtt caagcgatcg cggtcgacca gcaactcttc cttgcgggtc
    15001 ccggactgac tgatatcgat cgccgggaac aaacgcttgt cggccaggcg acgatccaag
    15061 tgtacttcca tgttaccagt tcccttgaat tcttcgaaga tcacatcgtc catgcggctg
    15121 ccggtatcga tcagcgcggt cgccatgatc gtcaagctcc cgccgttttc aatgtttctg
    15181 gcagcgccga agaatcgttt cggacgctgc aaagcgttgg aatccaatcc gccggagaga
    15241 acctttccgc tgggaggggc aatcgtattg tatgccctgg ccaaccgagt gatgctatcg
    15301 aggaggatca ccacatcctt cttgtgctcc accagccgct tcgccttttc cagcaccatt
    15361 tccgcaacct gagcatggcg ctgtgccggt tcgtcgaatg tagaactgat gacttcggct
    15421 ttcacttgcc gctgccagtc tgtgacttct tcaggtcgct catcaattaa caggacaata
    15481 agcgtgactt ccttgtgatt tttcaaaatc gcgcgggcga tcgcctgcag cagcatggtt
    15541 tttccggttc gcggcgcggc gacgatcagg ccgcgctgcc ccttgccgat cggcgtcgtg
    15601 agatccatga cacgcgtgca gtactcttcc cggtcaaatt cgagattgag gcgctcttca
    15661 ggatagaggg gggtcaggtt atcgaacagg atcttgtcac gcgcgacttc aggatcctca
    15721 tagttaacct tctccacctt gagcaaagcg aaataacgct cgctctcctt gggcggacgt
    15781 atttgccccg acacaatatc gcccgtccgg aggttgaatc ggcgaatctg tgagggggag
    15841 atatagatat cgtcaggccc agggagatag ttcgagtcag gtgcgcgcag aaacccaaaa
    15901 ccatccggca aggtctccag cactccttcg ccaaacacaa caccgttttt ctccgtctgt
    15961 gcttggagaa tggcaaaaat gagctcctgc ttgcgcaggt ttgccgcccc ctcaatcttc
    16021 agatcccgcg ccacctcatt caggtcggca atcgacttct gcttgagttc cgcgagatgc
    16081 ataacttccc ctttaacctg tgtcatacct cttccctatc gggctctcaa cccgacagct
    16141 ccccaaaatg agaaaaacga tttagtggtc atgaacgaat tcacggaagt gattgtctag
    16201 atgtcgacct gctggttgtc tacggcagga tggttcgatg aaggtgttgt cgatttagat
    16261 tttgcggaaa tgaaaacaac ccactattgc acaactgccg ctgcactgcc cttgggaatt
    16321 tatgcttgag atgggtctcg gaagctgatg cacagaaggt aaagcaattt ctacaaatcg
    16381 cgaagttgag ctaattttag cgccgacccc gaacattgtc aatagattaa cgtctgatcc
    16441 gtcgtccttc gaaccacaac acaccttctt actagcccca caacgctccg accgttacat
    16501 accagtctcc ttaataagta aaccccaagc gtggacgggc atctcggttg caggccgtgg
    16561 agtgatatgt taaagatcgc gcggggaaat tttcatggcc aaatcagaca acgacacacc
    16621 ggatcgcata tttacgttgg ccgaagccaa cgaactcctc cctcaactca atcagcgatt
    16681 tgcctcggta cgccgggcga aaaccgttat cgcgagcacg aaggaggagg tgagcaaagc
    16741 cagcgcgcag gccgcgtcag gcggcggaag ccccgttgga gcgctctata tccgagccct
    16801 ccacgagatc agcggaagcc tccaggcgat tcacgaactc ggcgtggtca tcaaagacgt
    16861 cgatctcggg ctatgtgact ttccatataa gctcgaaggt cgtgtggtct atctgtgctg
    16921 gcagttcggt gaggatgaaa ttcgatggtg gcacgagact tcctgtggat ataaagatcg
    16981 ccacccgctt gacgaaacct ctagctagca aaggactgtg ccctccatga cattcgtcat
    17041 ccttggcttt gatgggcccg acggccaagc aaaacgcaaa attcatcgca ccgctcatct
    17101 ggccggactg gagccattga accgcgcagg gcgagtactc ttagccggcc cgttcactga
    17161 tcaggcaggg agcctgatca tcatagaggc agattctctc gaagaagcca agcggatcgc
    17221 ccaagaagac ccctacacag tgcacggaat tttcgaacgc gtggaagtcc atcccttcca
    17281 acaggtactt cccgcttcac aatcgcacac gtaatcaacg ttcttcaggg acccgaggtc
    17341 agttactcgc agcaagagta agcacctacg tcccactaac actgagtagc gtcccgcata
    17401 ttcctcagat tgacaacccg aaagatcgcc acctataatg cgcacttagc gccgtttgtg
    17461 aaggccgaat cactctagtt caaaagacct aacggccttc tccagcgctg gccctaaatt
    17521 tccccaaagt gggccttctg ccgtgttgca gagaaacatc gagaaagcat agagtgactt
    17581 gaccccacgc tttaaccgag ggtgtccctt ctccaagttg ttaaccccca aaagagagca
    17641 ggttcgaatg actcagtatc gcgtgttacc aggcccagag cacttcctgc caccggcagc
    17701 agcctccatg gggatctatc tccccaatcc aggcgaggca catatcaacg gcgtgatcgt
    17761 tcctgaagaa aaagcctacg aagaagcggc acgccagttc ctgatggcac aggtaccgac
    17821 cattttcccg ggccccttag tcttgtgggc ctggaacgaa aaggccgcta agaaggccgc
    17881 tgccgtcaga aagctgtacg aaattttgaa ggagtgcgtt cagcccgggc agaaaccgat
    17941 gctgattcct atgccggact atcgtcccaa gtatccgaag atcaatcccg aagtggaaat
    18001 caatcccaac cacccgaacc tgaccatctg gcataacaag attgactgct gcatgttcat
    18061 cggggtgcac tgccaccagg cgaatctgtc gcttaagatc atccggggcg gaacgtcctg
    18121 ctataccgtc gcgatgtgcg cccaggcagg acatgaagat gccatgctct cctttagaga
    18181 tgcctctgtg gagaaaatac tcaagctcgg cgaatggatc cgtaaactca agggcacggt
    18241 caaaccgcgg ctgaccacag caaagaccag cgcgtcaaac taagtggctt caagcacttc
    18301 taacgattaa aaggaggccg agtccatggc ggaaataaaa tcccacatcg ggacacagaa
    18361 taaaaagggc cagacgtata cagatgcgtg ggagatgatg cacgaggccc ctcgcaaccc
    18421 gtccttttat accggcagcg aggttattaa agaagccttg cggcgtgcaa gctgcgacgt
    18481 gatgattgcc tatcccatca cccctcaaag cgaagctgct gccctcatcg gcgaattatt
    18541 tgctgaaggc tatatcggcg actatttccg aggagagagc gagtttgccg ttatgtccca
    18601 atgcgccggc gctgcctttg gtggcgcacg ggtgttcact acgaccgcag gacccggcac
    18661 aatgcgcgcg atggagaact ttcctatgtg ggcaggtgcg cgtctgccga ttcaaatgat
    18721 cgtcacgtgt cgcggcatca actccccgtt gtcgattcag ccggatacca ttgagatttc
    18781 ctacctgtta aatacaggca tgctggtttg gcatgcagaa acggcacagg actttttcga
    18841 ttggatcctc aaaggcttca tggtatcgga agagccggaa gtgcatttac ctctggcgct
    18901 atgctgcgac ggcttcttcg tgacccatac gaaagacgtc gtgaacctca ctccagcaga
    18961 tatgtgccta ccaccttacg atccctaccg ctcacccgtg ccttgcatgg acatggagtg
    19021 tccgccggtc agaatgatgc gtgacccgtt cattatgaag agcaactaca tcagttatgc
    19081 cacccacgct tcatggcaac aagaaatttg ggcggcggtt gaacgctcgc gcaaacacac
    19141 catcaagtgg atcaatggcc tgatcgatac ggaaaacacc gatgctgagg tcgtgattgt
    19201 tgcgtcaggc actgcggtct cgcaggggcg agaagctatt cgcctgctcg aagacgaagg
    19261 cgtgcgttgc ggactggtga aggtaaagtc actccgtcct tggcccggag aggaaattcg
    19321 cgaggccacc aagaatgcga aacacatttt tgtgcccgag tttaacgtga cgggctggtt
    19381 ggcgaaagaa atcaaagcca ccatccctaa cagcgagcgt gttcatgctg gcccgcatgt
    19441 gtgtggcgga atgacaatgc cgccagaaat tattgcctca gagatcaaag cggctctagg
    19501 cttgcgctcc gagtcactcg ctggaagagg tagctgatag tagctgagtt gtgacaccgt
    19561 ccattgagcc atcattacgc gatagacaag ccttgaggag gcatacatga gcaaagagcg
    19621 aatcaaaatt tcgccggacc tatatgacat catgccgtcg gactatcagg acctcgttca
    19681 aagcgcgacc tatgggaaag aggaccgtgg ctggaaagat attggaacct ctaaagaact
    19741 gattgagcag cactccttgt gcgccggttg ccctgaatcc atggcctttc gctatatcct
    19801 ggcctcactc ccaaaccctg aggatacggt gatggtcggt tccacaggct gcaccagcct
    19861 cgtgtttccc atggtggctg tgcacaacat tcactccctc tttggcaatc aaaacgccat
    19921 tgcatccggg ctgaagcgag ccctgagcgt gagattccct gaccgcgtga aagacgtagt
    19981 cgtcttggca ggcgatggtg cgacggtcga tatcggcctg gacatgaccc tgcaagcctg
    20041 gttccgccag gaaaagttca cgaccatctg tttcgacaat gaactgtacg ccaataccgg
    20101 tggccaggag agcggcctga tgcaaaaagg cttcgttgcc aaaatggccc cggttggaaa
    20161 actgttcgac aaagtccgtt tgcctgaaat tgcccgagag tccggctgtc actacgtcgt
    20221 taactgcaca gtgagcaagc cgtcacttgt ggaaaaggtg attcggaatt ccgtcctcat
    20281 tgcacgtgaa attggaccta cgtatatcca gctgtacacg ccgtgcatcc ttgaaatcgg
    20341 caagaacagc atggaaggcc tccaggaaat gcgtgactct gaaaagccaa ctgagcgatt
    20401 tgcttataaa gagtatgtca gcgaaccggc gaagcagttc ctggctgaac tagctgctaa
    20461 ggacaaggaa cgcaaagccg ccgcaaaaca gttagcggga aaagcttaag gaatcggagg
    20521 tcgctcatga tcaagaagag actcaatatt cgcatgtccg ggttgggtgg gcaaggcgcc
    20581 gtgacggcag cccacgtaat ggccatggcc gccaaccgag acggaaagtt ttccatttcg
    20641 aatccatttt ttggcgctga aaaacgaatg gctcccgcag aaagttactg tcggatcggg
    20701 attgagcgga tctatgaccg gggtgagttg gtatttcccg atgtgatcga agtatttcat
    20761 cctcaagtta ttacgatggg caagagctac accatgccct tctactccgg tgtcaaagag
    20821 gggggggttg tcatcatcaa ctcggcccag cctctcttgt cagaagaaga tatcaatcgc
    20881 ctcaaggacc tgaacgtggc cttgttctac attgcgggaa ccgagctcgc gatcgaagtc
    20941 gccggtaccg agctctcgac gaatatgtcg atgatcggtt cggtcgcagg tattaccaaa
    21001 tgcgtctcaa tggaagctct cgacggcgcc ttgcaagaac gattcggcaa gaagttcgtg
    21061 gcatccggtg gtaccgcctc cttggatgaa gcgatcaaga agaagttcgc caagaaagaa
    21121 atgctattgg cgaagaacct ggcaacagtc aagcgagcct acgaaatcgc aagcgaatgg
    21181 gctgataaga acaaggtcga gttgcgggtt ggcaatcctg ccgttgcagc ttaacgagag
    21241 agaaggaacc acgtcgcatg tataacgtag cgcaagtgat cgacgagaag tgcgtggcca
    21301 agaagggctg ccgcctctgc atcatgtatt gcccggaagc caactgcttg gaccttaact
    21361 cgaccaaaat ggtggcggaa gtcacaattg atcgttgtaa gggatgcgaa ctttgtgtcg
    21421 ttgtctgcaa cgccgccaaa cacgaagcga ttgagatgca ggcagtcagc gccactgggc
    21481 agctgatgag ccacaagggc gagtccgcag gcctcgggca agcctaccaa gggtagcgag
    21541 tcgcgttgtc gcaaggcccc atagcgttcc acgctatggg gccttttctt gaactgagca
    21601 ctgagaggat cccatggaac gtgaagacaa tgtgctcgat gaactgcttc gtgatatcgc
    21661 cggcctgctg aatgaatatc ccaaggcgat tgagcggcaa gccgcagcca tacacgccgg
    21721 aggaaaagac cctgaattag ccgagaaact catcaaagcc gccgatacga tgcgggatag
    21781 cgggaatctc tacctcacct gggcaaaaca ttttgccgcg ctggcggaag gaaatacgga
    21841 cgcctcatca gacgaagacg agactgatga tttcggcatc taaaaaagtc tcccccttct
    21901 ctctttagtt ttgctagaat gccgacgatt atcttgaatc tctttgttcc ccggtagctc
    21961 agttggtaga gcagccggct gttaaccggc tggtcgcagg ttcgagtcct gcccggggag
    22021 ccacaataca aagggggctg acggaataac accgccagcc ccttctcttt ttgcctgatc
    22081 tttcttacct cttcactagg acgactgttt aggcaagggc ttcgaaacaa ctccactctt
    22141 caaaacaatc tcgcaaatat gatcgagccg ctcgatgtgt tcataggctc cccaagggtc
    22201 cgtcgcgacg gcgcacacac cgtgattcat ctgcccgaca atgtcaaatt ccagcgtccc
    22261 atcctgcttc aaccccaggc actctgccgt cacatccgcc agatcacgtg acaaggccgg
    22321 caatgcaggg accgtgcggc ctacacgcgt ataacgtgag atctcgggaa actccgcact
    22381 catcgcctgc aaatcgattc cggcatagat cgcagctacg atgtgggtcg catggacgtg
    22441 aacgaccgtc ctcgtcttct tcgtgtccct ctgaaggttc cagtgcatcc acagctcccc
    22501 ggatggctgt tgcccatcac gcacctttgg cacaagttgg ccggtcacag gatcagtcag
    22561 aacctccaac cgcacgatgt gttctggatg gacaatcgtc tttcgccatc cggacggtgt
    22621 gatgtacagg tacttgcccg cccgtttccg catgctgatg ttaccgtctc gggtcgtaat
    22681 ccaacctcgt tcatacactc gcctcatcac atcaccgatt gcagtcagca tacctgcctc
    22741 cctggggaac caactttcca acttctgtta tcggttcctc acccgcaacc cttgagggga
    22801 gaaggcaccg gcccgacaga aaatgaaaag tgcgcaggga gcggcatttg tttatgatga
    22861 gccggatatg actgcgctag atggattcat tcccgcactc ctgctccgta atccgcatct
    22921 catgacattg atacctcgct actggtctcg ggaggcgata ggagcacccc tccctcaggt
    22981 gacacgtcga ttcactaccg aaccgagaac ccagctgcag ggcttctgtc actggcagtc
    23041 tcacccccgg agttccccga ccctcatcct gctccacggg cttgagggct ccgcagactc
    23101 ccactacatg cgcgggatga cgaccaaagc ctaccgtgcg ggatttaacg tcatccgcat
    23161 gaaccaacgt acctgcggcg gcagtgacca cctaacaccg acactgtaca acagcgggct
    23221 cagtaacgat tatcgagcga tcatccagga attgcgggaa cgcgatcacc tgtctcggat
    23281 gtggctagtg ggatattcga tgggcggaaa tctcgccttg aaagcggcag gagagatggg
    23341 acagtctgat ccgacgttag caggtgtggt agcggtcagc ccgaatattg atcccacgct
    23401 gtgtgtggca gccctcgagc aacccaggaa ttggctctat cactggcact tcctgtcgag
    23461 tttaaaggcg agaatgcgaa gaaaagcagc gctctttccc gacacatggg atttgtcgcc
    23521 gttgggttcg atgaaaacga tcacccagtt tgacgaagcc tacaccgccc gggacggagg
    23581 gtatcgggat gttgtcgatt actacgaccg agcaggagcc cgtcacgtgc tccaccagat
    23641 cgccgttccc acactgatca tcacggctca agacgaccca ttcattcccg ctacgatgtt
    23701 cgataccccg gcgatccgac acaattccaa tattacactg atgatgctgc gctacggagg
    23761 acactgtgga ttctttcaga aacggtgccc gcaggaggac cgattttggg ctgaaaatag
    23821 aatcctcgaa tgggtaggtc ggatggtcac ggtatagaca tagcagtctt ccgatatccg
    23881 cggcatatct ccatacccat ttgggagaga acagagtcgg tcggatagcg aagccccacc
    23941 gccagttact cttgggcgtc aggtactcgc tttgacgggc gcaggcggct tgccaaactg
    24001 ctcatccaac ccgaggtgag gcctggcgag aaacgctccg gccagctttc ttccgtcatc
    24061 gaaggcaggc tgcgcagcct gccactccgc gataggtgga ttcacgggaa gattgaccat
    24121 gaatctcgta ccatggccca cctcactggc tacctcaaca taccccccat gctcctgaac
    24181 gatctggtgc gcaatggtca agccgagccc ggttccctca cgctcgccgc tctcgtgctt
    24241 ggtggtatag aacggatcga agatatggtc tagatcctgc gggtcgattc ccggcccact
    24301 atcctcgact tcgatctgaa cccaaggctc attcaccggc ttgacaagtt tgcgagtgcg
    24361 aacggaaagg cggcccccct ggtcaccgcc gatggcttcc atcgcattga taaataagtt
    24421 cagcaagacc tgcttgatct gctgccggtc cagtttcacg tacggcagat cgctcgccag
    24481 ttctttctga atcgtgatgg ccttgctgct cgccttcacc tcgataaagt agaggcagga
    24541 cgacaccacg tcattcaagc tttcctgagt aagcttcggc gtcatataac gcgcatagtc
    24601 cagaatctca tgcaccagcc gctcgatccg ctcaacgtct tcacacacga cctggctgaa
    24661 ctgctccata aactctacat cgtcacggcg ttcaggggcc agctgaacga acgtcttgat
    24721 ggaggtcaac gggtttcgaa tctcatgggc aaacccgccg gccatggttt caagcgagcg
    24781 caaccggtcg gtacggcgca tcaacaactg ggaccgacgg aggtcctcat ataagagcgc
    24841 attgtcgatc gcgatcgccg catgctgcgc gagggtcttg agaagggcca actcttcggt
    24901 cgaatacatg ccatgattgg cccggcggcc taagttacaa aaacctacca gccggtcctt
    24961 gctgatcagc ggaatacaga cctcggactc caacgctttc aaggtgtcga gcaagcgcag
    25021 atgctccgta tcggccttgt cgtattcgat ttcttcccga acaagggcgg tctctgtccg
    25081 caacaaggtc ttgggaaatt ctccctccat cttaatcgga ggcacgacgg cctcacgcgt
    25141 cacgaacccg taggaggcgg tcaagctata gatcccttgt tccttatcga gaacatagac
    25201 ggaggccgtt ttaatgttca tcacacgcgc gagcgtttgc acaatttctt ttgtaaggga
    25261 tttcagttca aggatcgcca caagagactt cgaaaactgc accaacgtat cgtaggcatc
    25321 gtagcgctct cgaaagagcg tctgcccgat cgcgcgctcg acccccttct gaaggttcgc
    25381 caagccgctc gatacgatca caaataaacc gaacagcacc ccggccagaa gataatggaa
    25441 cgtcccggta aagatccgga tgaccagcaa gatcatcatg taggccggaa tcaggcccat
    25501 caacttgatc gacaacatca ccttcggccg actcgggacg aacgacacat ccatcaactc
    25561 gtatcgagcg atggcatagg ccacgacggt gaccgagagg ccggccagta aatacccgac
    25621 cggatagaac cccacgccat aattctggag gaaatcgaga gaggcaagga atccaagcgc
    25681 gagggccaca accgtatatt tcagctgagc gccgagcagc ggggatgaac tgaggtggag
    25741 ccgatacccc tgatacagcc gataaaccgt caaacccccg cccgccacca cgtatgcgac
    25801 cagccaggga tggagcaccc cggccttcgg atatcggccc caatagtaga catactgacc
    25861 atctaccaga tacggcgtga acaacaggct ccaaaaaagc gcgccgacgg cataattcca
    25921 cacaatcagg cgatcatgcg cagcccctcc ggagagactc tgcgcaaagt gataggtcag
    25981 cgcaggcaag aagaccactc ccgtgtaaat cacctgcacc cagaagagcg cctcatcctc
    26041 gcgtgacgcg gagaacagca tgaaacagcc gaataaccag agacaggcgg ccccgcacac
    26101 cgcgctgaag atcgtattcg acgtggaacg gggattgttg ctgagcaccc agagcgcaca
    26161 aatcaggaac agcgttccgg ccaacaacgg aggggtggaa tgtccggtcg cgcgattact
    26221 gagcaccgcg ccgatggacg tcgccacgac aataatcgcc agcacgatgg catatccgag
    26281 ccccttgttc agtacgacgc tgatattcat cagccggtac cgaacgatcg tgtacgccat
    26341 cagagccgta tacatcgtga tgagcaccgt gccgtagggc aacagcggta cgccatacca
    26401 gagagggaaa ttcgtggccc cgcccgcata cccgatgatg ctggcggcca gcatgtacgc
    26461 atattgatgg cgccgaatgc cattcgcatc ccgaagggcg accccgacga gcgaggttgc
    26521 gtagacgaca taccagagga aatacagcag gaaaggatgg aagaagaggc cgggaaccgg
    26581 ccaaaaagag aacgacatcg cagggtgcac cccggccacg aaccagggcg tcgtatcggc
    26641 cagcagaaac agtgccgcca gaccatagcc gatcttcagg gctttggcgt ggcgcgcggt
    26701 aatgtcaaga aacgtgacgg tgtggtgaaa atacagaatc gggatgagag atgcgccggc
    26761 catcaccaag cgcagcagac tcagcgcaag gtctttggac tccgtgagtt gccagccaca
    26821 gtagaagacg ctccaaaagg caatgctcag acagtacagc ccgtaaatcc ggttgcgtgc
    26881 agcggcagga tcccggaagt agacgaacat tccgagccct gtcgcagcca acgcattcac
    26941 cagggcgctg aacgcgagca ctttcatctc aggtactctg gtaaagtcga ggccgtgtca
    27001 tcatgccgcc accgtgaggt tcgccaggcc cttatcggcc acggcaatga gcgcttggtt
    27061 tccgaacgtg gaatagaccc gcggatgcgt gacgccggcc gcgcgtaaca aactttccag
    27121 ttccgcctgg taatggaaat gaaaaatacc ttcgccttcc cgctctttga ttctaccaga
    27181 attgtccagg aggcgacgcc cttcttcgat ttggtccggc gtctgagccg tctcaacgaa
    27241 gctgcgataa atcgccgaga gatcggcata cggcttcagg ttggaaatca ccaaccgtcc
    27301 attgggagcc aacacgcgca gatattcccg gagagtgaag agcgggtcgc gcacgtaccc
    27361 gatcacaaga ttgcacacga ctcggtcgaa ggaatgatcg ggaaacggca gcggagcctc
    27421 caggtcggca cggcagaccc gcatcgacat cggcacatag gctcgcagcc cctcataaaa
    27481 ctgcccttgt agcgccgccc ccacctgctc aaagttggtc atggcctgcg ccagagccgt
    27541 cgggacaaaa tccaccccga catagtcagg cgagcgaaaa tctccgcgcc tggcatagcg
    27601 ctgccgaaac gcctgattga gctgcaagaa cacaccgagg ttaccattgc cgcacccggc
    27661 atccaaaatc cgctccccgt gatggcaatc gcccatcagg cgatagacat gatccatgag
    27721 ccgccaaaaa tcggccacgt tcgggatcgt ctggaaattc tgtaagtact cctgccagaa
    27781 cgccacatga tccgacttgc ccaccggccg acgcttcttg ctgcgttcgc gttccgcgcg
    27841 gttctgcacg ccgatttctc gatgcgatgg ttcgaccatg cgcgccatcg accgtgtcgg
    27901 ccacaactcc tgctggcagc atagggcaat ttggcgatag accgtgcggg ctttccgggg
    27961 gctctcttga agtcgatgca acgcgcccgg caccacatag gagtggcgga gattcgcacc
    28021 catcgccgcg ccgactgcct cgatggaggc cggatcgacc cacgcatcct gctcggcatg
    28081 aaacagcacg acgggagcat ggaggtgctc ggcgtcctgc tgagtggtgt gcaggtcggc
    28141 atagcctccc tgcacagcat gctccagcca acgatccgca tcgatggtca ggccaagaat
    28201 gttgaccacc cctttgcgca caccggccag atgttcgccg atcagatcct cttgatggac
    28261 ggcctgcagc gtatgctgaa catccatgat tccattgatc atcgccagca gccccaggtg
    28321 agggactcgc cccgcgacct tgagcgcgac gcgaccggcc aggctggtcg ccacaagacc
    28381 gatggatcga cccggccatt gagcagccac gtagtccagc acggtctcca gatcggtctc
    28441 catatcctcc aggcgaaact gcgtgaccaa cccatcgctc tctccgacat gattcacatt
    28501 gtcgaagcgc acgacatgaa acccattgcc ggcaagatag taggccaacg gcacatagtc
    28561 gcgcttgctt tccccatacc cgggagccaa caccacaatc ggtgcgtcgt ccgacagatc
    28621 ggttcgaggc cgatcgtgac agagcgcgat acggtgaccg gacgggcgga cacactccga
    28681 gaactcactg atgacggaag gcccttccgc cctgtcgacg acgccctcgc cctgctgctg
    28741 cccgagcagg tgctcaatga tgtcctcagc ctcttttgca accgggagaa agcgcacccc
    28801 cgccaacacc gtccgccccc caacctgccg atagccggaa ggagcgccgg cctctcccgg
    28861 tcttgtccat accacttcac ccaccacgga gcagccgaca gacggcgtgg acacagaaga
    28921 tcctcgctgg gcgacagtcg atgacaccca tttcagttcc aattctgaac ccagcaaatc
    28981 ctcttcggcc gacagacaca gacaggcccc gtttgacgtc aaatctgtcg tcaacgcccc
    29041 cgacaaaatt cgatcaccgg ctctaagccg gagactgacc tcggtgctca atccgaccgt
    29101 aatccgctgt tgacgcctcc gctcctgacg tggacgctcg ggtacccggg caaggcgcgc
    29161 gcgcgcggcc tgagcgacga ccggctgccg ggactcggtg tcgataaggg catcctcact
    29221 ctcctgggcg atcaatgcgg cgacgagatg cagccccttc gaccgtgcgc ggccctcgct
    29281 catccacatg gctactacct gctcatcgcc agggctcaac cgcgcaaact gaacggcaag
    29341 tgtcacccct cttttcgtct gacctgaacc aaggacactt tcggaaacgc gaatcccgca
    29401 cacaacccca acactttcgg gccgcgtggc cccgggagtc acactcagca gaatctgctg
    29461 attcagcatg gcaggaatgt cctcggaaaa ttccatcgag agcccgccta agctgaggct
    29521 tttcgtctga ccgttccacg tatgatcctg aaagtgaagg agggtcgaga tgcgactctc
    29581 gaggcgtcgg gaagcccgcc gctctgcaac agagagagac caggcatcgc tggttttctt
    29641 ccgatcgggc accgaagatt gccgcctgct ctcctggcca agcaggccaa tcacagacac
    29701 actctcattg cgcgcagcga tgtgcttcga catacattcc ctcccgccgt attgagtgct
    29761 gcgcgttgca cagcatcgaa ttgaataacg tgtggacgtc gtcgcccgct cacagagctc
    29821 gctccgggcg aggctggact cttgcaagcg cacgaacgat tccaaaaatg cgggaggatg
    29881 tcggctgatc taacaagggc aggattaacg acacgcacgg cctcagaatt ggcacacagc
    29941 gactgtcata caacccaatg ttcgaagctt attctaggga attagacata attgtcaact
    30001 catccgcaga aaaaaaatgt agttacctac aaaaattaac ttagatgata gctacaggga
    30061 agaaagacac cgttggccgg gtcgccacga cgcggaagca gccccagcaa gtcagaacga
    30121 tgacgaccgt catgatcctg acgccgattc ctcacttcgc gtaaaggccc cacacaggtc
    30181 cggcaagacg ctcatcgccg catcacagcc cgagccatta aggaatttca cgcggcagaa
    30241 tttgccctcc cgaccgggca ccggctcacc gatgacggtt aatgccgacc ctcctcagtg
    30301 accgacatcg tggcccggcg caagctggtt cgacgacaag gcaggcatcg gggaatcggc
    30361 ccgggttggt tgctttgacc cggcaggtat gctactcaca atccccgata ggtcagcctg
    30421 agcactcacg atgcgcctac ctcatattcg tgctcacagc ggcatgctga tccccgtcct
    30481 ggccggaatc atgctgggct gccacagcac cccgcctatc accgagggct atcctcatca
    30541 gcgggcgctg caaggaaaaa gtaaagaaca ggtgctggcc tgcgccggca cacccctcca
    30601 ggaacgagag gatggagcgg ttactatgct ccggtactac aaagaggccc cgctgctcga
    30661 agagtccatg gtgtcctcga agagcagccg tcctaccgta catcacggct gctgggcaac
    30721 ggtcgtgatt cagcaccaac gagtcgacca ggtgcagtat cgcttcgtgc cgagttcggt
    30781 cgatgcctca aacgactgcg aagagatctt cgccaattgc ccggaatgac ggcccggcga
    30841 caccgcagtg ttggcccgcg ctggctgctg ttcttcatgg gcttgctgtc ggcaatgatg
    30901 ccctggacgg aaccggcgct cgggctggaa aagtacgggc gcccgctccc ctccatggat
    30961 gaaccggaac atgaagcgcg cgaggaagag gaaacgctgt tcggcggcta tctattaacc
    31021 ggtgcctttg taaagaatcc gacgttcgcc gccaggcctg acaataccgg actcgtcggc
    31081 atgcgccaca tgctgcactt ggaaacggac ctctataagc agtacctcac gttttacacc
    31141 gatcagaatt ttttctcgga ccgcaccgac ggatggatca agttgacgga atgggacggc
    31201 accttcgcct tcaccggcgt gctcgatcgc ttcaattggc ggctgcagta cgaacgagat
    31261 gccccgcttg accgtcgtgg cctgaaacag gcctacgccg atgcgctcgt gacagcccgg
    31321 tttcaagcga ttcaggactc cacctggtgg cggcgcacct tcccgaacca gaatctcacg
    31381 gcctatgccg gcgcaggatg gctcttccac aacagccaat acttcgcccg ccccaacaat
    31441 acgggccgcg cattgttccg gtacgtcgcc catgccgacc tggacgtcta caagaacaag
    31501 gtcgtcctgt acggggatat gaatctcttc accgaccgcg aagcgagcaa caccctgaac
    31561 ccttccgaac tcgattggat catcggcatc gccgtccgat ggaaggatac ggaattggcc
    31621 ttctaccgcg aagaagaccg accgctggac cagtccggct tggtccaaaa gtattacgca
    31681 gtccaattgc gctttgcgtt cgatgtgtcg aaaggcgcgc tggaacggat gggactgaag
    31741 cggtaggcgt tagcaggctg cgaaaaaact cgattgttgc gcaatacacg acgatcaact
    31801 catgcgccct gtcggtattg cactaacccg caggatgcgc aaaaaggccg tccagcaagg
    31861 ccgcagtaag cgaagagacg aatcgtactc gcgccgtacg gtgagcctct gagcgccgcg
    31921 agaatgccgc tggcggactt tttccgcatc ctgccagact atcgagcggc gaggtggcta
    31981 cgcacatcct gcacgtaggt tttgaacggg tcagccataa aaaacggcgc cgttccgcgc
    32041 agtttaaaat tcgaccaatt gatgatgtac tcgcaggcca tctgttcttc gaagtcgagc
    32101 ggggcatccg acacgatccc tccgcctccg ctggccagta gatgcctgac caactcaccg
    32161 cgtccgaacg cccgcgtgtt gcgaggcgga tggtcctgcg cgagatgaac gatcttatcg
    32221 gtcgtcaggc gcggtcctcg cccctcctcc tcgagaccgt agcagagtcc tcgctcagga
    32281 tgcagattgt gatattcgag gtcgagactc ttcaacatgg gatcgtccca cgcacactgc
    32341 tcggcctccc gataggtctc caatagccac agtttcgagg cccaatcgat ccggccgacc
    32401 agcttttcat acccgccacg caaatcggtc agcacggatt cccattgctc cagcacccaa
    32461 tccgtctctt cgtcctgccc cttgcaatgg cgctgggccg cggccagaaa ctgttcctga
    32521 atatcgatgg cggacatcga ctgtcccgac tccaaacgga cgatccaacg gcgatccggg
    32581 tcgcgcgaaa tatcctgcag ggcctcgacc ggctcgtgga tcccaaggcc gcgcggggcc
    32641 tgtccggcct caatcagttg cagcaccagc ccggtggtcc ccatcttgag cgccgtggca
    32701 tattcggcca tgttcgaatc cccgcacagg agatgaatcc ggcggtattg gttcggatcg
    32761 gccagcggct cgtcgcgtgt attgacgatc gcacggttgt gttgaaccca ctcgaaaaag
    32821 tcgttgacga tgtagtcggc gcgttgggag atctggaacg gcacaatcga tcgatcttgc
    32881 gcatcccgca gcccaggccg atgcaggatg agccccccga cttgcaccca ctcgttggga
    32941 tcactcgcgc atccgatgcg ccccgccccg gtgaacacct gacgggtgac caagaacgtc
    33001 accagtggac cgaggccgcg ccgggaaaag ggaaactgtc gcgtgacgag atagttttca
    33061 tgtgagccga acgtcgcatc ggtctcgtga tcgatattgt tcttgatgag cgacaccgtc
    33121 tcgtccagcc ccagcgccct caccgcatct tgaataatcc gatcccctgc acgatccgag
    33181 gccacaagat ccgccagggt cgtgcactcc ggagaggcat attccagatg ccccatatcg
    33241 atgtagatac ggcccgcatt ggtcagaaac cccccgttgc ccggcggctc gtcatgacca
    33301 cgatggtgaa gatcgaggac tcctcgccgt tcgacatgaa aaatgtggtc gcgaatacgc
    33361 tgcgccaccc aggagggcga atggtcggga cgatcctggt tcaccaggag gccgtattcc
    33421 gtttcaagtc cgaagatacg attcaacata ttaggtggtg gctggtgcgc tgacagccga
    33481 aggcaacaga cggcccagtt cctcaggacg aagcgcgcga tatttggccg atccaggccc
    33541 gcggcgctcc agcagaacac attccaatga tttctcggca acggtctgac gcacatgtgc
    33601 cagcaacgct gccttgtcag ggagactcga accgtcccct tccgtctttt cttccgatgc
    33661 ctgggttgag gaagactccg cagccgtgga ctcggacgcc gcttgagcta acgagcctac
    33721 tgcccaggtg cacagggcga gttccacggc ctgcgccagc gaggcctgcg caaacgccgg
    33781 ttgcgattca acatagcgtc gcatccggcc ctgcacggaa gccgtcgcgc ccaatgccgc
    33841 ccagccctgg ctctcctcga aatttccgtc gtagttgatg gacagaaaca ggtttcgctc
    33901 gggtttcacg cccaattcgg cgagcaaaat tttggcgatg aagggggctt tataaatttc
    33961 ttcaaaggcc tgcttgatgg tcggtgccag cccatacttc atgagcctgg cgcccgtgac
    34021 atcggacggc gatcgattga acccctcgac atgggccatc tccaggaggg agaagcgcag
    34081 cttctccaga tccgccgggt gtcccattcc acccagcgca atgcgatcgt aaatctcata
    34141 cagcttgggc gttccgcggc tgacggtcag catcaatatc ccgtcggcat aggccaacgc
    34201 gacgaccggt gagccctttg caaattgttc atcgagatac tggcgtcgat tgccgaccgc
    34261 ctcgatccac cgatagggtt cttcatacat aacgtgccac cttcaccata ggggtcaata
    34321 accagccgtc tatctgttag cgtccgcgca gcacttcgct ctcatgcaat cgtttcaagt
    34381 cggcatccgg aatggtctgc acaccggacg ccttaatgag tttcaccacc ggatagagat
    34441 tcagctcccg attcaccccg ccggtggccg aatcgaattc cgccgcgctg gagagcaacc
    34501 ggagcgcctg gatcgtggcc tgttcctccg ggagggttgc caatggttgc gggccccatg
    34561 tgttcacata atgtaggatg cctcgaatcg tcggtgagcc agaaccggac acggcatact
    34621 ccacggcttc aaactctgca cccagaatat cgtaaaaata aatcttggca gactcctgct
    34681 catggtcata tccggcaaag accggaacca cggcaccggt ccctgccaac gctgccggca
    34741 cgttctcctt gagcaacttg gagaccgccc gcagttttcc ttcgaaactc aactcctgca
    34801 attgcgtgcg ccggtagtat ttgaatgaat gctccagcac ccgtaccatc tcatacgccg
    34861 tcgccggcac ccctgcgatg gccatcacac tgtggcgatc gatctccagc accttgtcgg
    34921 tgcggtcgta catcactatg ttgcccgccg tcgcccgacg atcgcctgcg accaacacgc
    34981 cgtcgcgata cttcaatgcc agaatcgtgg tggccgtcgg aacgtccatc ccggccgccg
    35041 ctgcgacagg attcccgaac tgatatccct gttccttcaa gagctgaaaa aaatcccctt
    35101 gcatccccat tgcttacctc gttagacgtg aaacgtaaac gccaaaggtg gaccaacgat
    35161 cgcttcaatc ctcagccccg cgacctttca cgtttcactt tttacctttc acgtttattg
    35221 tcccgtgcgt tgccgatagc gctccgcttg cttcggatcg actttccgca tccgcttcag
    35281 cagattgtcc ttatccggcg agcccgtttc aggccgccga ggccctccgc cttcttcaga
    35341 cggccccgac ggtttcggca tgggatcgcc cggcgcctca cgacgttccg gcatcaacat
    35401 atatcgcatg gttatccgtc ctttcgtctc gaccacgccg ccagcgcatc ggctggagac
    35461 gacgcagctt ccaacaacgc tgcacaggcc tgcaccgcat ccggctcaaa gaggtcaccc
    35521 atctctagcg tccgcgcacg cagccccccg gaaaactgca cccgctccca ttgcatcccc
    35581 gtgatctgct ccgggaaccg tctcacacac aacccgcgaa gcccgcccct ggtatcggag
    35641 gggccggcca ccagggcctg cgcaatgtca cgctcgctcg tcatgcgcca ggttttccct
    35701 tccgcttcga ggcccagaaa caggcctcgg tcggggttca cattgtgata ttccaagtcc
    35761 aaactcgcga gccatggatc ttcccacgcc agccgctctt cccgcatgaa tgtctcaagc
    35821 agccactgct tggtcaccca gtcgatcttc ccgactaatt gcgtgcgatc ctgacgaagc
    35881 gcacagagag tctcctgcca ttcccgcaac acccagtcgg cgtccggatc actgcccgca
    35941 cagcatcgac tcgccacgtt ccaatattcc tcttggatcg ccagtcccga tatcgtccgg
    36001 ccatccttca atcgcaccgt tgtcttcaaa tcaggatctc tggagagctg cttgatcgca
    36061 gccaccggct gttccaattc caggcttggg gcctctccac gttggagcat gtccagcacc
    36121 agcctggtgg tgccgacctt gagcgccgtc gcgtattcgc acatgttcgc gtcgccgagg
    36181 atcaggtgca gacgtcggta cttagtccgg tcggcatgcg gctcgtcacg ggtattgagg
    36241 atgggccggt tgtgcatcgt atcgacgcct agttcagcct ccataaagtc ggcccgctgc
    36301 gaaatctgga agggaccggg cacaaacccg gactcttgtg cctccatccc caccttcccg
    36361 gctccggcca ccagctgccg gctgaccaaa aacggcaaga gaccgctgac cagctgagca
    36421 aacggcaggg agcgcgacac caggtaattg tcatggcagc cgtaactgtg cccgtgaaag
    36481 tcggtattgt tcttatacag ttggacgtgc gcaccgccga gttgctgatt ccgtcgatcc
    36541 gcggcccgct gcacaatgcg ctctccggcc cgatcatgcg ccagcaaatc tttgagcgtg
    36601 cgacattccg gagtggaata ttcgggatgc gtatggtcgt tgtaaaaccg cgcaccgttc
    36661 ggcaggacca gatcgctctt catctcgtga aacgaaaatg gtcgatgcgc atcaaccttg
    36721 acgaagtcgt cctcttcctt gtcctgttgc agaccggaga ctcggaaacc gcgcgcgtcc
    36781 tcatgcgggt cctcacctcg atagtcccag cgccgttcaa acttgccggg caagtgggca
    36841 cggaccagtt ccatcgattc ctcgacgggg tcgacggcat ctagatcctc gcgggtgatg
    36901 ccatactccg tttcaatgcc gaagagatgc ataggccgtg tcagataatc tggttgacca
    36961 atcgctcttc cgtcggccgc ccgcggcgga aggacgacac accgacaacc tgttcaggat
    37021 ggtgatccag cagtttgagc cattcttcgg cggcatcgtc cggcggcaac atctcacctt
    37081 cgcgatattc ctcatgcacc gcatcgagca gatcttgcgc gcggatcccg tcgccggcag
    37141 gcgcccctgc caccgcccgc tcaatcgcct tctccttcgc ccgctggacg atcgaagaga
    37201 gaatcgcgcc gctgacaaga tcgccgcgat acagcacctt attctgaccg ttccgaaggc
    37261 gaatggataa cacgcgattc tgatcggttc gtgtaaacag actgccgacg acctgctcaa
    37321 tcacggcgcg acgggcagct tcatggtcct gcccgctttg ctccagcaac tcacgatcaa
    37381 gcggcaacga cggcgtgaga tacaccgcaa gaatctccac ggccgcctcc cgactcggcc
    37441 gcgcgacctt gatcttccga tcgattcgac cgggtctgag gaccgccgga tcgatcaaat
    37501 ccggccggtt ggaggccaga atgatcacca catcatgcag cgactcaatg ccatccatct
    37561 cagcgcagaa catcggcacc agcgtattgt tgatgttgaa cgaccgcatc gaccgcctcg
    37621 tgcccaacac cgactcggct tcatcgatga aaataaacgg cagcgccccc tccttccgtc
    37681 tggcgcgcgc cttggcaaaa aggtcccgga caatccgctc ggattcgccc agccacatgt
    37741 tcagaatctc cggccccttg atgtgcagga aggcgccgct tgtcaccggc gggttcttcg
    37801 acgtgccatc cgcggcagcc tgcccctgcg attcacggac gagctgagcc agactggccg
    37861 ctgcggcctg tccgatcaag gtcttgccgc agcccggcgg tccatagagc aggaatccct
    37921 tcggctgcgt gaattgatat ttcgtgaacg tgtcggcatg gagcagagga tattcgatgg
    37981 cttttcgaat ggcttcgatc gcctgatgct ggccgccgat ctgcgcccac gtcacactgg
    38041 ggacttcatc gagaagatgc gtcctggcct gacgattctc gaatttttca atggcgatcc
    38101 ggtgtgtcgg ctcgatacgg acttcgtcgc cggctttgag atcggccccc agcaagtcgc
    38161 tggaccgctg cagaatcaac gcctgccggc ccatatcctg ctcgaaacga atgcgcccat
    38221 ccggcaacac ctcggccacc ttcagcaccg gcccgttgcg gtcatatccc agggccttga
    38281 tcaccgtata ggcttcattc accagaatct gcgtgccgat cttaagatcc tcgaccggga
    38341 gacgcgggtc gatgttggcg tagtactccg cccctccgac gacaatgcgc gccacagctt
    38401 ccgccggcac ttcgagcagc aggccgactc ggttagcagg cgccgtcaac ttggcgacca
    38461 cctcattgag tttcttcaac tcggtgtcgc gccggtcctg ggcaaccgac tgggccgtaa
    38521 tcacatgtcg taatttatag agcagcttct ggcgagggtc gccgtctgga aacgccgcca
    38581 gacattcttc tatcagttcg atcggatcag acgaggcaga ggcctcacgg cgcggcggct
    38641 gttgcccgcc cgactctggc tgatccggat ttcgatggtc gctcatatca attcctccgg
    38701 ctggtccaac tccggtcatc ctaacacaga gcacaactcc actgtcgcgg tcgcacgggc
    38761 cggttcacac ccgcccgatc aattcaattg accgcctgca gcggcgccat cacttggagc
    38821 cgacgtttgc cccggacata gtcgatcgtc acctcatcgc cgactttgtg cttctccatc
    38881 agatccatca aatcgtcgat ggtttcgacc ggctggccgt ccaccgccac gataatatcg
    38941 cccagttcga tccggccgcc gacggtctca cgcgccccat gcagcccgat ccgttcggcg
    39001 atgctccccc ggccgacctt tccgatgatg acacctttga caccccaacg ccgggccatg
    39061 gcatccggca ctagtgagat gcccagtccg ggacggatca gctttccatg tttgatcaat
    39121 tcaggcacga tccggctgac ggtatcgacg ggcaccgcga acccgatgcc ggcgaacgct
    39181 ccgctcgggc tcatgatctg ggtattcacg ccgatcaacc ggccgccgct gtccagcagc
    39241 ggccccccgg aattgcccgg gttgatcgcc gcatcggttt ggatcacgcc ttcaatcgtc
    39301 cggttgctca tcgacttgat cgtgcggccg agagcactca ccactcccgt ggtcagggta
    39361 tggtcgagcc cgaagggatt gccgatggcc agcaccttct gcccgacacg cagcgactgg
    39421 gaattgccga tgatcaccgg ctggagcgcg gtttctggag cctgaatctg caagaccgca
    39481 agatcgtgat ccggatcggc gccgacgact ttcgccttgt attcggtgcg atcggccaag
    39541 gtcaccgtga tcgcatccgc gccgtaaatg acatgatagt tggtgacgat gtggccctgc
    39601 cggctccaca cgaagcccgt ccccgagcct tgcggcactt cgaagaggtt gaacgaccag
    39661 ggatcgcgct gcatcgctgt gttcgcaatg aacaccaccg accgcgtagc gcgttcaaac
    39721 acggcgatgg tcgcctgctc ctccggactg agctcagtcg gcgcggcaga ttgcgcgatg
    39781 agaagcccct tcgtgccctc ggcggcctga agccaggccg gtgacgacag cgccagcgcc
    39841 gccaccaaac caactctcaa ccgtttcccg cgccacctct tccggatcgc cccgctcaga
    39901 cgcatcctat tcaccgatga cctgcaacac caggtccctg gttcgtgcgc gcgtatcgaa
    39961 atccaacacg acgatctgct gccaggtacc cagcgtcatc accccgtcgt tgaaaggaat
    40021 ggtgagcgat tgcccctgca attgaccgcg caagtggctg tggccgttgt cctctcctgc
    40081 gttgcgcaca ttgtgctgcc agcccggatc ggccggaatc aaccgttccc acagcgctct
    40141 cgtatcggcg cggatacccg gctcatcctc gataatcaac acggaagcgg tcgtgtgttt
    40201 gataaaaatc gtgagaatgc cggcatgtaa ttgcgtgtga ctcaacgccg cctggacctg
    40261 tccggtaata ttctccaccc ggcaatcgcc ctgcatcttc acctgcatcg acatgcttct
    40321 aaccggcatg tagtgactcc tgacccgccc ggcgcaggaa cgtacgctcg cggctggaca
    40381 acgaccgccc cctggcctct tccaatacgc gatcgaatac cccttccgcc gcaagctccc
    40441 gcaaggcctg caccacggga cgactgataa tcccttcacc ttgcagagca tccagatagg
    40501 cgaaggcttc cgacacatct tcacgctgcc gcacatagta gtcgcgcccg cgacgctgat
    40561 tgctataggc ctcgaactgt tcgcaggcga ccaggatctc cgccaattgc cgcacccagg
    40621 gctgcgcgcc tttcagcttt tccggatagt agtaatacag catgatccgg ctcatccacg
    40681 gggaccaggt gaccgcaagc tcccgcagac gcggtttcac ggcgcgtaat cgggcggcta
    40741 atctgcgggc gtacccaagc cgcatttcca cctgctcgca ggcccaggga ttcagttcga
    40801 tgccggccgc ttccatcgcg cctcgataac gcgaaacaaa ggcctcggtt tcccgtccat
    40861 aacgggtctc cgggtggata acacgccatt cacgcggcct ggtgggaatt ccctgcgcgc
    40921 gggcccagga ccagatccgg ccgaagagcc gtcgatccag tccggctcga cctaaatcat
    40981 gcagaatgca ggcgatctga tactgtttga ttcgtgccgg ctggtgtccc atgcgctgag
    41041 ccacagcggc gcacatccgg gccgtccgga gggcatgcgg acagtcgtac cccttaatca
    41101 gccgtcctgg acggcgcggg tcgggatagt cgtagagatg cagcagttga gaaacgagtg
    41161 tccgcggaac aagcagcgga ggggacgtca tacgtgtagt tgccatgcag gtgtgatgag
    41221 tcgcagtcga tgatcgaacg gcagaatatc gtccgtgaaa agaacgtgtc aagcaaacca
    41281 cgacccgggc agaggatttc ctcgcactca ctgtggggct ttgttattct gaggctgatg
    41341 ctgaccgaca accgggaggg gatcatggcg aagacgcggt ccatgcacat tgttgtggga
    41401 agcgcctgcc tcctacttgc tcaagccttc acgcccctgc tctccgctgc agccgaactg
    41461 attgatttga gcggcaaagc cgccgcattc gtcaccctgc gtagccgtga taatttttcg
    41521 agcgaatacc ggtacgatgt gacggtccgc aacaattctg ccgatctctt cattgccgac
    41581 tccttgatca tcgtcctgga tcgcataacc aatatcggcg ggcaggatcg tgagaacctg
    41641 acaggcgaat ccctcctgaa cagaatggaa atcatcggcc aggacggcac cactgaggac
    41701 ggaagacctt tttttcgtgt ccctcaagga tcggggccgg acctcacccc aaacagcgag
    41761 agcctccccg tcaccgtccg catcaggaac cgggactatg tcgcggtctt taccccgtct
    41821 ttccgggtga tggggcaaaa acgggacgca cctaaacaga aaagcccggc ggcggccgct
    41881 gccgccgcgg ccgcaccgaa caaggccacc acggacaagc tgattcaact cctgatcaag
    41941 aagggcctga tcacgccgca ggaagcccag tctctgagcc aaccatgagc agccagtcgt
    42001 gcaaggcctg tgtcggcacc tggcccgacc ggagtcattt catcgccgat tgcggactca
    42061 cccgggccta cctgcatgac gaccagttct tccccggctg gaccgtgctc gtcctgaaac
    42121 gccacgccac ggaactcttt cacctctctc gcgacgaacg cagccgcctt atggaagaag
    42181 tgtccggggt cgcagccctg ctcgcgcaag agtggcaggc cgtgaaggtc aactatgagt
    42241 tgctcggcaa ccagcttcct cacatccatt ggcatctgat tccacgcctt cctcgggatc
    42301 ccgcgccgct cgaaccggtg tggcgtattg cgcatgaacc ggtccgactc gaaccggacg
    42361 cactcgcttc ggaaatcgac cgtcttagga agatctggcc gtttcatcca aaccagtccc
    42421 ccgtaaaccc ctgagatcgt cgactggcca cgatggcgaa tcagcctccc ccaccaagcg
    42481 cgaataggcc tcgcaccttc ctccgggctc tgctctggtc tctgccgctg cacgtgctcg
    42541 tctcgatcgc cgtcgtcgga ggggctgccg cctattacag caacacagta tcctcccatg
    42601 gcacatgggc cgcagtgggc agctggtcgc tgacggccct ctacgtcgtc ggtgcgctgg
    42661 tcggcggcgc ccttgcggga gtgttgagcg cagcccaacg aacggtggag cggctggagc
    42721 tcacgctccg ggactggctc catgccttgc ctgccatgca gtcggcgtca acttcggtag
    42781 gacgaagtct tgccacggct cgacaggaat acgagaccat ggtcgaccga tgcgtgactc
    42841 agaccgccgg ccgtctgaga cttccgcgtt ggctggaaag gctgattcgc gcggcgctgc
    42901 agggcgtggt tgtggatcgg ttcatgacct cctgcacgga ccggggactc aacctcgtcg
    42961 ccccgcagga attcagaaac tggttgctgg cggaaggcgt cagcctgggg ttcatgccga
    43021 ttcaggatca attgtcctgg tggcgctccc tcatactggg cctcctgggt ctgctggtcg
    43081 cgattgtcct cgcgctcgca ttcctcaaca attgaatgct ccccgcacat catcccggca
    43141 cacgtttcat gcgagatccc gtggaatttc cgcttcgtct atgtgtataa aggaacgtct
    43201 tttttcatgt ggaggattgc cagcacttat gtttctgcac cacgccacca agggagggtg
    43261 gcctttgatc ctggccctgg cgttgctcat tcacccggct tgggcgcgcg cgcaagaaga
    43321 cccctccgcg atcccggccc cggaaccgat tcgagaagag ccgctccccc ctgaatccgt
    43381 cgctcagccg gcattgccga cgccgattcc ggcgccggtg cccctcagcc tgttggagac
    43441 cctgacccag actcgccaag ccttacacct cgaacccgat gcccaggagc cacgcctcac
    43501 gctgggccga accctcttcc agctgggcga taccgatggc gccatcgacg aataccggac
    43561 cgccctacga ttccatccga ccgtggcgca ggcccatctc gatttgggca ccgcgctcat
    43621 ggccaagcaa gactggcgca gcgccatgac ggagctccag gaagccgtcc gcttggatcc
    43681 gaccctcgtg caggcgcact acagcatggg aacgattcat tacacccgcg gaaatgtaca
    43741 atcggccatc aaggcctatc aggaagccct gcggcttaaa gcggactttg ccgaagccca
    43801 ctatcgcctc gggctggtct tgaaagtcgc gggacgggac aaggatgcgg cgcaggaact
    43861 ggaagccgct gcgctggcgg gattggccaa agctcaatat tttctcggca acgcctatcg
    43921 ctcaggccag ggcgtagaga agagccagat catggccatc acctggtggg cgcgggcctt
    43981 tgagcagggc ctgccggaag cggctcaggc gctgacccaa ctccggcggc tggcagcggt
    44041 caaaggcaat ctgcagacaa aacaatccaa ggcggcggcg gaggccttca aaaattattg
    44101 cgatcagctc tggctggatt tccccgatct ggatcggggg gaccaggcca cggaaaccgt
    44161 cggcaccaca ttactcaagc aaggccgcac aggcgaggcc ctgccggtgc tgctacggga
    44221 agcctatgcg ctcaacgaca tctcgcatgc ggcgctggtc cagctctacg aacaaggcct
    44281 cgacggccag cttccccccc atggccaatg gattctgagt tatctggaat ccaccgcagc
    44341 cgatggcgcg attccttcac gcacggccct ggcccgcatc tacgccaaag ggctcggcct
    44401 ggcaccggac ctcgccaagg ccaaaagtta tctgaagggg atgccgcggg aagaggtcaa
    44461 acgtattctc gacgaaatca cccctgagac tccaaaaccg tagtaagctg ttggacatga
    44521 tttcaatagc ctcgccatgc ccgccggcat ggctcacggg caggagcaga accgctcgca
    44581 ggatgttcgc acagtccgtc cgacacagcg cacagcgcca ggcgcgcgat gtgtcggcac
    44641 atcgacggac ctggttacca tcccgctagt cgtcaccacc acccatgcaa atagccactc
    44701 gcctgttcgt caggaatgtc tggctgcaag ccgtcgtcat ggcattggcg tccctcgccc
    44761 tcaccgaggc catgtgggcg ccggcgcctg tgacctacca cgcgctcgaa tgggcaccct
    44821 acgacacctg gatgcgactc cgctcgcaac cggcccccga ctcacacctg ctcctggtca
    44881 tgagagatca ggccagcgaa caacagttcg gtgccggcct ttgggatcgc agcctcccgg
    44941 ccaggctcat cactggattg cacgatgcgg gagcagcggc tatcgggctc gatattcccc
    45001 tcgacttccc cagccctccc aacctcggcg gcgccgtcag cgacgccttg ctgatggagg
    45061 cagtgacatc cgccggaacc gtctcctacc ccgcatttcc aactgcgccc atcgatcagg
    45121 atagggtagg ctttgctgtg cccgcacagg gcctcacgcc gggccgaagc accctacagc
    45181 cgctcctgga cctcgaccgc attgcgcgcc gggccgggct cttttatgga aacggatccg
    45241 acgagcttcc agccctgggc ttctccttag ccaccacgtt ctggcagatt ccattgcgcc
    45301 aggtggagcg acgctccgga caggttcgag tgcagaacgc acggtggcct ggcggtacga
    45361 caggctccct gacgatcccg ctcgatcgtc agggccgact cctgctcaac ttcgccggac
    45421 gacaagttcc cacggctttc cccggcacca ccgtgttgga actttcccgc ctcctcgacc
    45481 ggaaggataa tgaagcgctc ggcacgctgg tcaacggcaa agtcgttgtc ctgctcatcc
    45541 aaccgcactc gcaaccggaa cgcacgctcc cttcaggcga cgacgcctcg gatatgctcc
    45601 tccaggcaca cctgctccac acgctgctca cgcaagactg gattcacgaa ctctccccac
    45661 ttcagaggct actggtcacc tttgccctct gtctctgggt cgcctggtta gtcctccggt
    45721 ggacggattg gaaggggctt ctcctgggag ccggaagcct cctgctgtat ctgactttcg
    45781 ggctggtctc gctcaccacg gcccattggg ttctgccgtt tgtgattcct gtcacggcag
    45841 ccgtcaccgt gctcgtggcg accagcctgc tagggcaggt cgtggcaacc cgacgcctgg
    45901 ccttgctgga gcaggacatg ctgcaggttc agcaaaacct gggggcggtt cgcgaagcac
    45961 tgatcttcag ggagaccgca gtggaatccc tggaggaaga cctcgaatcg gctcgcgccg
    46021 gcatgtccca ctcagcctcg agagaagccg aatcgacgag actcgccgcc gatcttcagc
    46081 tcaaaatcgc cgatgcccag gcgcaagagg aggcgacgcg gcagcgcatg aacgagctcg
    46141 aacggcagct tcagagcctg cgggccgcaa ccatcagttc agtgcccctg ggaaatgtag
    46201 aacaggaaac actccgccgg gaatgcgagc aagtgggcat cgtcaccgga gccgctacga
    46261 tcctgaccat gtttcgtgat ctcaagaaag gcgcccgttc caccgtcacg gtcctgatca
    46321 ccggcgagcc gggaaccggc aaagagttgt ttgcgcgtgc cgtgcaccgt ctgagccccc
    46381 gggcaccgaa gccgtttatc gccgtgaaca tggcggcgat ctccccggaa ctcttcgaga
    46441 gtgaactctt cggccacgtg cgcggcagct tcaccggcgc gctgatggat cgcaaaggct
    46501 attttgaatt ggcgcatcat ggcaccatct ttctggacga aatcggcgac cttcgactcg
    46561 accatcaaag caaactgttg cgagtgctgc aggaccggac cttttatcga gtcggagcga
    46621 cgactcctac cacggtcgat gtccgtatcg tggccgcgac caacaaagat cttcagcgtg
    46681 gcgtgtcgga aggctggttc cgcgaggacc tgtattttcg gctcaagggt ctcgtcctgc
    46741 acctcccgcc gttgcgggac cggcccgacg atattcctcc cctcgcggaa gcctttctcc
    46801 gggaagccgc gaaccagccg cagcacgcga acatgaggct ctcggaagaa gcgctggcga
    46861 cgcttcagca gcaggcatgg agaggtaacg tgcgagagct gcgtcaatgt ctcgaacagg
    46921 cgatcgcgct ctccgacgga ccgatcatca caacagccga cctccgcctc tcggggcaac
    46981 cttcgcccac cgtcgtcgtg acgggagccg aaccgctcct ccccgatccg gccggagaca
    47041 tcgccgtgct gaaccagctg cgccaacacc ggttcgatat gcaggcgaca gccaaggccc
    47101 tggggtggga tcgcagcacc gtcacacaac ggctgaaggg cctctgtttt caggcactcg
    47161 tggaatcggg aagagatcag acaaaagcag cttcggccct ggcgggagac ccctcgttac
    47221 tacgcgcggt cgaactgaaa ctgatggact attacggcca cttgatggaa acgatcgagc
    47281 ccttcaccac cgcggaggac gccctgctcg attgtaagcg tcgcttcaag aacctccccg
    47341 aacggcattt caaatccgtc gaagtcctgg tccggcaaca tttcgggtag aatacctcga
    47401 ctcaccgaat ctcatcccgg accagaacca catttatgaa caacaccttg tttaaaattc
    47461 gttcagacat acgaagcacc gcaggcaagg tcctttcatg agcaagacac ccttcgtgct
    47521 ttggaccgag tcagggcagc cctgctccgc gcgctggcgc tcggaatggg gagctccacc
    47581 gcccgcgcgt gtaactattg cggatgacag tatgaccgcc gacgctgcgc accgcctggc
    47641 ctgcgaggga accgcgctgc tctggcgggg cgatttccag aatgcccggc agttactcaa
    47701 cgccatggcg cggcgtgccg accgccatcc ccccaaacct ggcacctcgc cggcagagtc
    47761 cttccatttt catcgacaga cccagtctcg tcgcgcgcgc atcttgggta tgctcctggt
    47821 gccacttgaa gaggactatg caattccgct ccgccgcgcc cccgaccttc gactcgcctg
    47881 tacggaagcc tatggcccct gtgaagggca ggccctggtg tcgcttcgag aactgctggg
    47941 actggtcggc gcacacgaat ggcggaagaa cggggttgtc gtccctgccc tcggtgatcg
    48001 tatccatccc cactatggag tattttcgcc ggttcgcagc gagtatgtag acctggtggc
    48061 cgacgcgccg ctaccttcca atgcgctcgc cttcgacatc ggcaccggca ccggcgtgtt
    48121 ggcggctgtg ctcgcccgcc gtggcgtctc acacatcgtt gccaccgacc aggaccctcg
    48181 cgccctggct tgcgcccgtg aaaatcttgc tcggctggaa ctgaccgatc gagtggaagt
    48241 cgtacaggcg gatctttttc ccgagggcaa ggcgccactc gtgctctgca acccgccttg
    48301 ggtcccggca cggccgacct ccccgatcga acaggcgatc tacgacccgg aaagtcgcat
    48361 gttatccggc tttctcagcg gtctggcagc tcacctggaa ccgggaggag aaggctggct
    48421 cctgctgtcc gacctggccg aacatctgga attgcgaacc agggcagagt tgctcgccgc
    48481 gttcgatcag gccgggctcg tcgtcctcgg caagaccgat atccggccgc atcatccccg
    48541 cgcctccgac caagcagacc cgctccgcgc cgcccgcgcg gcggagatca cgtcgctctg
    48601 gaggctgggg atgcgctaag ggaagggatg acgaataacc gtctcacaaa ttcaggtttc
    48661 attgggagct cattgtgata ggaaattttt gttcgcgcac cacagccctg ttcttgcatg
    48721 tcgacttctc aggggcacgg ctatggtttt ttgaatgcaa gaacgatctc tcgtcgcatg
    48781 cggatatcca tggtagcctt cgatatccca cgtttcggcg ggggtaggta tcggcgattg
    48841 gcaggcaaag ctctggattg ccgtgatacg agcgagagac cgtgctcctg cgcaacagcg
    48901 gtaacaatcg aggagttggg aatgaaggtg cccctgctcg tggagtcacc gacaacgtat
    48961 accgcacgtc caccaggccg caggacgcgg gacacctcag ctagtgcttg actcatatcc
    49021 catacatatc tcgctaaaag agcatggtcg tggttagaga gcgctggctt gaggccaagc
    49081 tccctgatga gcgacttcac gcacggggat tccaacgctt ccttcgaaga cgcctgggca
    49141 cccacgcttt ccccacgaat ttggcgaagc tcgctcacgt tgtgtcccat ccacacaagc
    49201 gaaaatttgc tgcaccgcat atagtcgatc gcgttcaggt atggcggcga agtaagcacg
    49261 aggtcaattg aaccatcttc attgtctagc tttcgtgcgt cacccctttt gacgactgtc
    49321 gcaggtccaa cggccactga tcccagttgg ggacagttcg cgacgactgt gttcacagcg
    49381 gaaatgaagc gattgaatgg tttgaccggg gcatgtgtga actcccgatg gggccggctg
    49441 tgggataagt ccatagcaag cgaagccccg gcgctcttgg taatgataag ccgggaaaag
    49501 ccgcaccaaa gtacgtcgcg ggttgcgtcg tcatgaactt ggcagatcgt catggaaaga
    49561 gaggtcagct ggcggcgagc atagtcatcg aaccaatacc gaatgaactt gcgagtctca
    49621 tcgtcgctgc ctgacggata ggcctgtccg gatgacagcg gcttaaatgc caccttcgcg
    49681 cggtccaaca cttctatcgc cttgtccttc actcgcatcc catcgactgt tctcgtccaa
    49741 acgcctgcga gcaggacggc tagtggatca aggtccatgc cgaaagcccg atgacccttg
    49801 gcgcgcgcca cagcgagaac ggtgcccgag cctgccattg gatccagtac acggagcgga
    49861 gtttcactct tccccaaggc ctcgagtgcg attccaggcg ccatacgtgc gggaaatggg
    49921 tgaatagggt tacgtccgag agcttgaagg atggttttct tgtgagattt gagcatgtgg
    49981 caattggctt tctgtatcaa ttggcaaacg agggctacga caacttatgc gccgttcggg
    50041 ctgaaaatgt atggtttgat agcattattg accggcctct ccgggtcctt ccggcataat
    50101 gatgtcatcg ctatgaagcc gttccagctg cgcctcaatc acagccgcaa ccttttcagc
    50161 gcttgcgttt atgatgattt tatcgccatc aacaccgact atgtcagggc tagatatcga
    50221 tagccccttt gctatggcaa ttacgtcctc gtcttttacg ctcgcagcat ggagggaatc
    50281 tcggcagatt aacgctatgg ttccggccgg caatgcgtcc ggaatcttgc ctttgagcgc
    50341 ctttagtgcc tttaggaaga tatctatcgt taatggccgt ttttcgttca gccatgtctc
    50401 caggtccgct acccattgag atactttctc tggatcgtac aacctgaaca cttcgcgaaa
    50461 ctttgtcagt gggatcgctc catgctctac ggtatactca agtagcttcc caaggtcaga
    50521 cgctttaatt ggagttactt gctgctgttc gcaacgcacc gcaagcgccc cttcgctaaa
    50581 ccctggtgct atgacgagcg cataatctgc ttcgtacctc tttcgatgtt ccacaatacc
    50641 atcgagctta atatttccag ttgctgcggt ttcgtgcttt gaagacttag catcgaatga
    50701 aaagctatag aggggcgggt ttgggttttc ttgtgtcggg ctgctggatg tgggaatggg
    50761 gtaggcacgt gctattcctt caggttctcc gggcttagca aggtcttgga cttcaaatcc
    50821 aatgtagcga agcgcatcgg atattaccgc ttcaagccgt ttactgttac cactgtcatg
    50881 ctgggtctga agaagtagct tggcaatgta aattccagat tgtcgccgct gcatcgcacg
    50941 gtaacgtagc agcttgtcgc ggtattcacg aatgttgttc agcaccgagg gagagattcc
    51001 tagatcaagt gcatagacat cggaaagtaa gctcaccatt gccatggttc tcagcaattc
    51061 tttttcagcc ttcgttcgtg tgtgctcggc tacaaatgga tgttccttgt ttacgactat
    51121 cgtgctgtct gagattctga atttaacaaa actgtcgtcg ccggaattct cgtacttaac
    51181 ttcacccaat gcggttttga tattttcagc cgtattctca cgccaagatt tccgtttccc
    51241 ctcacgatct gtaatgccag tctcgtcgaa gagacctgga agaggtggtt gcgttcttag
    51301 agtttccgaa acgacatttc tgagggggtt gagtgagaga actcccaatg accgaacgag
    51361 cacatcacct ccatcaggga tcgttgcatt catgtcacta tcgtagtgag ttctggcctt
    51421 gttaaaaact cgtcgcagaa atgcacgaaa gatctttagc gcacgtagat ttctgaattt
    51481 ttcgcggtcc gtcgtaagaa gttcattgag accatctgct cgggcagtca ttcggaatct
    51541 agcccatgcc gcatgactta gattttcctc cccaaagctt gggtcgttct ggtttacaag
    51601 tcggccgagt acgtttacat aaaaaccatt cgaggttccc cgcgcctcgc tctttccccc
    51661 actaatccga tcaacgaaca accacactcg gcctgtgatt atcccaatgc caggaatttc
    51721 aacgtgtggc actggagacg ttcccgattt aatcggaacc ttggtaagct tcgctggaga
    51781 gtcggaagct tcatctgaat cggcactatg attttgagat tgcccttcca cgccatagcg
    51841 ttcgtcaatt tcgatgctat cgatatcaag ctccgggcca ataatccatt cagccataga
    51901 aggtgtatta atcttcgagg aggctagcaa ctcgccatta agacaaatgg ccatctcgct
    51961 tccgaatggc aatgctgatt caagcatacg acggagtacg ccgatcttca gctcacggcc
    52021 tgcgggcttc aggccagata atacgacaag agtccaggta ttggcggaag gccttgtcaa
    52081 ttctgtttta ggtgacccaa attcatcatc gctgagtttg ccatcaggtt gaggaatgcc
    52141 ccctctaatt agtttgagaa ttgcctgtcc gtcgtagaca gacgcaagag cggcttccac
    52201 ctctagctcg gcaacttcgt atagttttat ggtgaggtca ctgataaggc gatccgtgac
    52261 cgctcccttt tgttgatcaa tatccccata gtccatcgtc acccgacgaa ttattccatc
    52321 tgatgccttg caaatgtagg tcaatttgga tgccagtaca tacgtcgcaa gcttgccgat
    52381 tccgaacttg ccgatgatgg gtcgaccatg ctgaaagggc ttgtcttttt tgggggagaa
    52441 agcgatatgc cacaattgac gtaagccctc ttcatccatt gagctcccat tgtctaggac
    52501 gcacattgtg gctttcggat cgttgagatc ggtagagatc cgaacatcta cacaatcggc
    52561 tccggcgtcc caaccattcg agatcagttc ctcaaatgct ttttgtggtg acgaatatag
    52621 ctgttcgcta aagtgttcta gaaagcgagt gcttagctca actgaaatat ccccggtcgc
    52681 gcaaccgatt gtctcgggaa agctcgactc tgcttggcgg ctctttttca cgtccattga
    52741 cctcagtgga aagctagttg atgcttaaat gaaacacagt gcgcggccat tctactttct
    52801 tgaggtcacc ctggtccatc cctagccatt ccctctaccg accactattg ttcggattgc
    52861 cttatttgcg ctcgtgagat cctcttccga accaacttga ccttcttcca agccggtaaa
    52921 ttgtccgacc ctcgggtacg cgcatcaacc gagcccgtcg ctattccctc tcgatgttaa
    52981 aagtaggatc ccaatcggcc ttaaccgcgc gtatcgatac gaatgttcta tcaaccccgg
    53041 cttgttccct cctgatagga gacggcctgt ttggtctcca actgcgcgcg tccaacgagg
    53101 gtcttctgag accgcgcgtt gcgcgagcaa agagactaaa cagggtacct ccatctcgga
    53161 ctgcccggcg gtgtctttca agaacccaac aggcacctct tctcctattg ttcttagttc
    53221 ttcaagggag tagccgggtc atccttcact gcgcgcatcg gacgagcact gtttcatcgt
    53281 gcgcgttctg cgtgtcaatc atcagccgaa gttgaccctt ttattctgat gtgaaattga
    53341 cccctc
//
LOCUS       DAORRQ010000026        52117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_37_length_52117_cov_30.887711, whole genome shotgun sequence.
ACCESSION   DAORRQ010000026 DAORRQ010000000
VERSION     DAORRQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 52117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52117
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37_length_52117_cov_30.887711"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..591
                     /locus_tag="PK224_10045"
     CDS             82..591
                     /locus_tag="PK224_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16119.1"
                     /translation="MYRARHLFDICILLVGLFASGCSSSATYIRATVPDHPLDRVAQQ
                     SQEQWSVEKVDANTLSMSDGWPAYSFGALGYGASHANLSFDPTTSELDVQYYFKAFPI
                     MTLWIPFSIDAEPGAFGAALKPTMNDQINEIIKWSGGTIISRRAGDRSEPFPPKSKGK
                     VSSLSPATP"
     gene            690..986
                     /locus_tag="PK224_10050"
     CDS             690..986
                     /locus_tag="PK224_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16120.1"
                     /translation="MALLLAFYLIFSTVIPASALEDSPATAALVIKADVLYWEEDELV
                     VKEFSGHEERLKVTPETKIEGVVGGRLKTGDKIVAQITDDRKALSIKLQVPDGR"
     gene            complement(1106..1384)
                     /locus_tag="PK224_10055"
     CDS             complement(1106..1384)
                     /locus_tag="PK224_10055"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB16121.1"
                     /translation="MLELSTTATFDALFSKLPKPIQRKAASKTDLFRENPFYPSLHTE
                     KLRPKHHEVWSFRVDRSYRIIFKFLGPNQVQLRYIGHHHSIYDYDMFK"
     gene            complement(1392..1589)
                     /locus_tag="PK224_10060"
     CDS             complement(1392..1589)
                     /locus_tag="PK224_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16122.1"
                     /translation="MALAAKVVKRTKDSFQFKISKENFESFCDSIGLYRREFLDALDT
                     SEKDHKAGRITKRKSLRELIA"
     gene            complement(1662..2378)
                     /locus_tag="PK224_10065"
     CDS             complement(1662..2378)
                     /locus_tag="PK224_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16123.1"
                     /translation="MKRIPLGTLLLFTTVILGGLLATSTPAEAHDWLGDCVVDFDNTF
                     ALTYIYGQARGNFGYPTGIDNSGELQPCNATHQACWTYRHRCFGNYINVDDMTYGHIH
                     LVFDSPSLLTPCFVDPGDGPGAGFGRNVGDDCIAADWIHEPRTLGTHGKDHWIKIWME
                     DRVTHQPRVFDMPIVRVTGTEPIQFWFRKSNGSWWYWSNLAPNPWLVGGWVFDVTEVR
                     IRGAQAANGPYFIGAFSILD"
     gene            complement(2419..2763)
                     /locus_tag="PK224_10070"
     CDS             complement(2419..2763)
                     /locus_tag="PK224_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16124.1"
                     /translation="MRRITLLMLLLYGCVPPPPHQFVPGGPLGEAGQTVQGPRQASDE
                     EVRAMLAESSLHQQNPPGRVDLNGLLTQITQATNFRQKQRLVDRYHHAALLLPEAERD
                     QAFRRLRDATGK"
     gene            3182..3937
                     /locus_tag="PK224_10075"
     CDS             3182..3937
                     /locus_tag="PK224_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16125.1"
                     /translation="MLASPLRVLVSMAIPVFAGLLVSCVSLEFDRMTGTAFPTSQTVN
                     GQTVTLSSIFDEAGIKLTVEQDDTGIQPLNIAQDECISDAELSTLENGHRHLSLFPTA
                     ACPFDFCNTYHLYGAVVNHYGEVLDVCIPEFILGKMWQGHTRSAFAIFYRMNTIQTDG
                     AAYFRTTAHEIGHAFNLHHSDGDGTSIMTQSDDLTGDPVYRFSEQSREHLANHPGQCK
                     FPGAIGAAPFTWVANDHAAWAHPLEELTITDCQ"
     gene            3954..4946
                     /locus_tag="PK224_10080"
     CDS             3954..4946
                     /locus_tag="PK224_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16126.1"
                     /translation="MHLDPIARRSRKVFLVCVVALLFPAVRANDGLAQEPPGSTTLQL
                     TLDIGQSALSLGEPVYATVRLVNVGTMPVEVFKLLDPQTGAIQIEVSSSNRPRFVFLP
                     LFYADAVHNRIALAPGEAVVAIVPVFYGALGWTFDRPGTYRVTAEYRPQGASQQDRIR
                     SHAVSVTVTDEHGAGASLLTGSSAGEEAAKFLLWQRGDQLQAGQALLTGLLTQHPESP
                     LADYALLAFGRNLSRSFRNYAVGKIREADCGSALTYFQRIRSDRLPVFLQIQQRLDEA
                     RCFIKLSQPAKALDSMERAEQLRDGRPEYTLLFQQAIRLEPTLGLHSAINTPHR"
     gene            complement(4991..6535)
                     /gene="hpnR"
                     /locus_tag="PK224_10085"
     CDS             complement(4991..6535)
                     /gene="hpnR"
                     /locus_tag="PK224_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HRB16127.1"
                     /translation="MNVLLVHPSPLMYTKIYLRLEPLGLELVAAAARRAGHDVTLIDL
                     QVESHASFVTLLQERRPDAIGFSCNYLANVPEIVDLARLTKQQLPHSAVFVGGHSASF
                     VAQAMLEHADGAIDCVLKGEGESAIGPLLTAIEEDRSALAEVPGTVTRYAEGPPARMI
                     TSLNEVHPARDLLRHRRKYFIGVLDPCASIEFTRGCPWDCSFCSAWTFYGRSYRAASP
                     DKIVDDLASIREPGVFIVDDVAFIQAKQGMAIGEAVSRRGIKKQYYLETRGDVLLRNK
                     EVFQLWKRLGLAYMFIGIEAMDEEGLAHHRKRTTISKNLEALEFARSLGITVAINLIA
                     DPAWDRRRFEIVRQWCLEIPEIVNISVNTPYPGTESWHTEARHVTTRDYRLYDIQHAV
                     LPTTLPLPEFYAELVKTQQILNQKHLGWNALKSTARIAAGHLLRGQTNFVKMLWKFNR
                     VYDPKLQLADHARAVAYQMPLPPAQKQDISAGLLYIHRAQGRKGRALDDSTEQFVEAT
                     RTGASA"
     gene            6693..7571
                     /locus_tag="PK224_10090"
     CDS             6693..7571
                     /locus_tag="PK224_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16128.1"
                     /translation="MSAQRLNRMSVRSVTGWWAVACLVFALSGLPVSVGAAVTTFIAG
                     DYAFTGPEQLDSGRQTVRLINRGRDVHQVQFLALPPGKTVVDVERALASRSPSLPNWL
                     RRHGGVNSVAPGDEASVSIQLDPGDYLLLCGIPDVAGRPHAMRGMVRALRVVEAAPSG
                     EPAPRADATLRLNDFAFALSGPLQAGSRTVHLVNDGPQAHEVVLIRLAEGASAKEFIA
                     RYRPGGLPNSAGREAGGLTGLDPGREGYVHLELEPGRYGLLCFLADPVTGAPHFSRGM
                     WMDIEVKQALPAAEGP"
     gene            7568..8539
                     /locus_tag="PK224_10095"
     CDS             7568..8539
                     /locus_tag="PK224_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB16129.1"
                     /translation="MTYRDLMRLANGYAESKVLLVANELGLFTAIGQGGKCADSLATT
                     CGASREGMDLLLQALAGLGLVRLKAGRYWNTTFGLNYLDGRSPTAVTNLLWLLNHHWS
                     DWTGMARAIRRGRPGWVPVTKTAAFRRRFALAMHERSYVLAPLTLDRIRLPRGARRLL
                     DVGGGSGAYSIALAQRHPDVHVLLVDQAVSVARRQIKEAGLADRIAVRQGNVLTAPLD
                     SGFDLVLISNLLHDFSEAENRGLLRRAHGALRPGGKIVIVEYFLNAAGTLPAEAAVFS
                     LLMYAFTPTGRSYAWNDVEAWLRGTGFGRFRRQLVTDSIGTLEAVRL"
     gene            complement(8529..9743)
                     /locus_tag="PK224_10100"
     CDS             complement(8529..9743)
                     /locus_tag="PK224_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16130.1"
                     /translation="MSAFSAAWTRRSGRAISRFRTSLQNLAAAHAPSGISNLIDHVTQ
                     QAASARPGADLWSGIAADCRRDLSGLSVHKGTLAQAVEWETLKLQTRLRSTNGYYPDS
                     VPLFRNRHVHIGTLIQLWRRLAFDTETWLAEQGHETLLDIGPWGGFNFVVDDDGYTRM
                     PFARLTLAVGSLPGTPLDDAGGPFFDHLLPCYRAELQAAGVHFPDQWQWQFPKRDQAG
                     RLAELSGTHYLPEHTYDRRTFIKVRLSRSCETAEEITLQDLLPLLERLHFTTDWDLYR
                     EQTQPVDARFDLQDFLSLNHVVEGLYQRTAKEERLLNEIKDAYRGAVRSPQVLYKYLD
                     TVIRSGWVENLYWALAEAALGVKRYQRAVSFDREVCPHIPPRLLIPVRRHLQRYHAGL
                     SAVAPAPTEVTA"
     gene            complement(10107..10235)
                     /locus_tag="PK224_10105"
     CDS             complement(10107..10235)
                     /locus_tag="PK224_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16131.1"
                     /translation="MHDPALWTSWYVWLLALPALVIGTLIWDWIQRRQPVRVRRRR"
     gene            complement(10506..11420)
                     /locus_tag="PK224_10110"
     CDS             complement(10506..11420)
                     /locus_tag="PK224_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRB16132.1"
                     /translation="MPATLQAFIAIFLLGALLRSSGLLGRIHAERLAFVVFSISLPAT
                     ILVSLDRVVFAPTAWKLPLAACLVTLSMLFCSWPVARLLHLPRATRGGFLLGTGCINS
                     VYFAYPVVLATLGDKGLAQAILFDLGQTTLTLTVIYGLAVWHGAKGTTARSAMRRFVS
                     SPPLWALCLSLLLTLLGLHLPGWLHDALTPIHLTTTPLASLVLGLSISFSAVRRTWAL
                     ALLGVGMRMVGGLLLGFAVAYGLDLAGMERAIVILVSAMPSAVTAVIFATDTGLDEDL
                     VTSIVALSICVGVALLPWLPQLVFLLAR"
     gene            11519..12757
                     /locus_tag="PK224_10115"
     CDS             11519..12757
                     /locus_tag="PK224_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HRB16133.1"
                     /translation="MAHPMTHPESSTQPRQAAVFFILVTVVLDMLSFGIIIPVLPKLV
                     EEFLGGDTAQAAEIYGLMGTSWALMQFVCSPIQGALSDRFGRRPVVLLSNLGLGLDFI
                     LMALAPSLAWLFAGRVISGIASSSFSTAGAYIADVTPPDKRAAAFGMMGASFGLGFVL
                     GPAVGGLLGAVDPRWPFWGAAATSLLNACYGFFVLPESLPMEKRAPFRWKRANPAGAL
                     MLLRSHHELFGLATANFLMNLAHGVLPSVAVLYLGYRYGWGPSAVGFTLAAVGVCAMV
                     VQGTLVRPITARLGERRTLITGLLCGATGFAIYGLAPTPLIYCLGIPVMAFWGLAGPS
                     AQMFMTRRVSASEQGQLQGAIASLTGIAGLIGPTLFTQTFAAFIGPQADWHLPGAPYL
                     LSTTLLLIGAGIAWRATRAV"
     gene            complement(12762..13775)
                     /locus_tag="PK224_10120"
     CDS             complement(12762..13775)
                     /locus_tag="PK224_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16134.1"
                     /translation="MHHRSALHHRSKPGLIRHIVISCSLLLLWGLSGQCADQAFSAEP
                     AAPAASPNTLSGHELLRIGTIHDQQEHFPESLTYYNLALSKFREKRQPQGIATTLVKI
                     ARIDERQGHLQQAHTSLKEAVSLFAKASDRSAHAESLLAIGRVSAQLGLSDESRDALT
                     QAAALFTRVRNARGWNDTMVQLGLLQIAQGENSAGLTTLQQASEEARTRRHVDQQFTA
                     TVALGDAHWLLGHTSDAHSAYLDALSLAEAEHHLPFEGMMQLRLARLDGDQASLKDRV
                     AQGKRALQIAQAVHDTAAEAEAWSLLADLYGQLGQATESQDADTHALAIYRNRELSVH
                     GNR"
     gene            13898..14668
                     /locus_tag="PK224_10125"
     CDS             13898..14668
                     /locus_tag="PK224_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB16135.1"
                     /translation="MSTTRATPKRARISLDRSIERLRKQLADLAAYLGRGLPTRSLEE
                     FDQDTENLIAELLGDTSELLEAYEYAEFGEAAGLVNLTDEAPESAGVESQRQRLLQRS
                     RVLESCIAELEARRAAEPKQSQASRQTLIGPQVAEHMSSEIRSLSQDASLKDAGQAME
                     KWKLGSLLLTDKQAYVGFITDSDLARAVVARGVDPATGVNVYMRRPVVSIAGDRPIIE
                     AVRMMKDQATRHLAVTQDGSIIGVISVSNILRYYSGVV"
     gene            14829..15188
                     /locus_tag="PK224_10130"
     CDS             14829..15188
                     /locus_tag="PK224_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16136.1"
                     /translation="MGPTKAIVQGQSLHEAVSGKLIRDGFTSQQAMEDYVRQHYIVMP
                     VRDNAGQPWQLDGKPVYCLHGVQYETVDDQKVHLSRCPDCGGMGIRADELTVDSDCIR
                     CTACGHEFDARLEMMET"
     gene            15512..16087
                     /locus_tag="PK224_10135"
     CDS             15512..16087
                     /locus_tag="PK224_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16137.1"
                     /translation="MAGATVTRPRKKAPQQNKKAPARKPKKARVQEPSGAVIVLSGAT
                     PLRRQKAAEVLAEELQLDLASVDLSAVVSKHAGETEKNVNRVFDMANRHGSILFFDEA
                     DALFGKRTQVHGAHDRYVNAEVSHLIQCIEDHQGLVILTSNGKSRIEEAFSPEKPVIV
                     LGGASKKKTATKPKPAAKSAPMPKKKVAKKA"
     gene            16111..16560
                     /locus_tag="PK224_10140"
     CDS             16111..16560
                     /locus_tag="PK224_10140"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRB16138.1"
                     /translation="MVLPLRSPREGLGGYVILPRLIDKVRLHALGALPSDYVRNLLKP
                     GLTLDGRFLKFTGLAADALQGAILSSPTDELVLAWVDQHAANHSDAEKQAWSEKAAAF
                     RPDPRVAKFLMASLPALAASVDITAYTLFDLIDMDEGRLPIPPNPAT"
     gene            complement(16598..16903)
                     /locus_tag="PK224_10145"
     CDS             complement(16598..16903)
                     /locus_tag="PK224_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRB16139.1"
                     /translation="MSQPGEGPSLGTETPASHHMIWIVYILECIDGSLYTGITNDLER
                     RMKAHATGKGAKYTKRRGPFTVRYIESLDSKGAALQREAAIKSLDRATKMALIASLP"
     gene            17335..17667
                     /locus_tag="PK224_10150"
     CDS             17335..17667
                     /locus_tag="PK224_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16140.1"
                     /translation="MIDRELAAAEVAWTQGNAGKARVCARRAVALAAAAWWARSGRSL
                     RHGDALEHLRRIQQQEAFPSPVQQAAERLTTAVTKRHDAPFTSDPIGDARLIVGYLAA
                     ATTDRRLA"
     gene            17735..18238
                     /locus_tag="PK224_10155"
     CDS             17735..18238
                     /locus_tag="PK224_10155"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HRB16141.1"
                     /translation="MVILVMGVSGAGKTTIGRRLADELGWQFSDGDDFHPAANLEKMR
                     NGHALTDVDRQPWLERMHAAIVDRISRNQPAVLACSVLKASYRAIVEEGCRAHLRLVY
                     LKGSFDLFHQRLIHRRDHFMPRELLASQFAVLEEPADALVIDAALPPNEIIRQIRLGI
                     PVDAAKH"
     gene            complement(18266..19654)
                     /locus_tag="PK224_10160"
     CDS             complement(18266..19654)
                     /locus_tag="PK224_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRB16142.1"
                     /translation="MEDRLTQLEELWPCFQGIIPAALSTCSEDGTPNITFISQIHYVD
                     PTHVAISHQFFNKTHRNVRENPLACAMMLDPRTLQAHRIYLRYQHSESSGPLFDSMSL
                     QLQAIASHAGMTDVFRLRAADVYEVVRIERVDGFTRLPPAIPADRPSRLFVDQDLESL
                     RLISERIHRAELLGDLLETSLRLLDDLFGFRHSMILLVDERNTKLFTIASRGYPENGV
                     GSEVGIGEGLIGTVARARRALQLSAIDHSLRYARAVRDCAEAGGGVDAVCREIPLPGL
                     PSAAGQIAVPLLTRGRLIGVLAVESAEPLAFMSREDTLLTIVASHLGGCIEQLSRDTE
                     ESPQGALARPQSSRALAAPPRSRSFCFFPQDDCVFVDGEYLIRNVPGRILWRLLTRHR
                     DEGRTEFSNRELRMDSWLGLPEIKDNLECRLILLRKRMEQKCPDIRLVPRGRGRFAIE
                     TDVAIALSERDT"
     gene            complement(19658..20113)
                     /locus_tag="PK224_10165"
     CDS             complement(19658..20113)
                     /locus_tag="PK224_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16143.1"
                     /translation="MIPQPVFDLLQTGVSVIVGTRDSTLMPESTRAWGIRAGKDRRSV
                     TIFLTEAISRKTIENLKENGLVAISCTRPTDHVACQLKGRVRTIRPADQRDRESSRDW
                     HREFVEELVAIGVPADLCEAWITSPALAIDIDVTDVFHQTPGPGAGEKV"
     gene            20292..21146
                     /locus_tag="PK224_10170"
     CDS             20292..21146
                     /locus_tag="PK224_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16144.1"
                     /translation="MLPTSSRWLTPLLVLLLAPAVVMADGPRAFAGSRAAGAPVVFVA
                     NDYGFTGPDRIPSGVTTMQIVNKGQDLHHIQLLRLLQGKTAADFRAALKAEPGRLPKW
                     IAFVGGPNAVLPGSEALATMSLGEGEYLLICLIPNKEGVPHMALGMQKPLSVQGLKST
                     LVSEPNAGLTITQADFRFALSRPVTAGSHMIQVMNHGTQPHEVVVLKLDPGASAKDFA
                     AAFEPGASGPPPGKPVGGVVGLETGAHAFFTARFEPGRYGLICFFPDPATGKPHFAHG
                     MTADFTVE"
     gene            complement(21240..21599)
                     /locus_tag="PK224_10175"
     CDS             complement(21240..21599)
                     /locus_tag="PK224_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRB16145.1"
                     /translation="MNDRQANSLARAWIEAWNRHDLDAIVHHYATDVEFTSPFVPALS
                     GEPSGTIRGREPLKEYFRKGLQAYPDLHFELLRTLTGVDSLLLYYRSVKGLLAAEMMT
                     VNGEGQIQAVRTHYVQE"
     gene            21738..22211
                     /locus_tag="PK224_10180"
     CDS             21738..22211
                     /locus_tag="PK224_10180"
                     /inference="COORDINATES: protein motif:HMM:NF021425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HRB16146.1"
                     /translation="MAPFMTQASPRNGLALIALFTLVKGVVLLLVGAGLLRLVDPEIA
                     TFLAPAVDLLHLHGHSRLLHSLLLKVGVLPSHTLFLMAYASLLYSVFLLIEGCGLWLE
                     ASWAGHMAVISTTVFLPVEFYDVLKQISVVHVTMLLLNVAIVGYLVAQLERRSLR"
     gene            22324..22716
                     /locus_tag="PK224_10185"
     CDS             22324..22716
                     /locus_tag="PK224_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16147.1"
                     /translation="MTEVNSMYRAQTRSDDLKAQIEKLEGLAHHTSVNPTFERFDAET
                     EDILMKIYGANHKYVESYKYATVGEAEALVNLPESAQEPQTRDIPKKGLQQRRQVLQS
                     ILTDLQRLEVQEAELLTGEDREDPPGPS"
     gene            complement(22945..23760)
                     /locus_tag="PK224_10190"
     CDS             complement(22945..23760)
                     /locus_tag="PK224_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB16148.1"
                     /translation="MPTSSHQRPTRRGRPGIALIGIVCLATSLIAASLLWAGSPSQTK
                     PGNGPADAEQGRAIFNGKGLCSTCHGIDGYRDRLPPQLSKNIRDNVEHLSPAPPDLRQ
                     AATLTLTTDKQRFEVIRHGHLRTAMYPLSKEALSDEEILALLPYLASIRGTVPPPAGL
                     PEPDTSLRGDAKSGRQLYHELGGCAICHGIEGHLNRRPPMSADLIKRLDALPAPPANL
                     RDSATLKSQNDEDRYRSIKRGHPGTAMYPKTLLRDEDIRDLVAYLATLRETGR"
     gene            23926..24657
                     /locus_tag="PK224_10195"
     CDS             23926..24657
                     /locus_tag="PK224_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRB16149.1"
                     /translation="MMYTAMNMTDHRQCVALSGCFRGKLCDQLMHRPGRSVGKGELVY
                     GLGDSAQSVFFLRRGFVKLTSLTEDGRELILRLHQAGEVFGELCHCTGERREQAVAME
                     DSEIVELNFDEFVAHLQNNRPAFLLFLSNVAQQLSAAYDQIQTLSFSSTMERLVRTLG
                     RLADEFGEPDGEWVRLTHYFRQDDLAQMIGARREVVSTLLNQLRDRGLINYARKDGLL
                     LHRAGLEGFLVSAEKSPANLSDSGF"
     gene            24791..25444
                     /locus_tag="PK224_10200"
     CDS             24791..25444
                     /locus_tag="PK224_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16150.1"
                     /translation="MQKLVRVLPVLMLTLSPIACAHSADSPAITVSPSATAHPAKVAE
                     TKAVLRDLWLGHILSIRNVAVATMDKNASARSAAEAGVVANAEQIARSIEPFYGKAAS
                     DKLFTLLAGHYGAIRDHLDATVAGAASRQEAAVKALTANAAEISTFLSGANPNLPKDT
                     LMGLLMAHAAHHLTQFQQLKDGDYIHEAETWTGMKQHIYVVSDALAQALAAQFPAKF"
     gene            25828..26412
                     /locus_tag="PK224_10205"
     CDS             25828..26412
                     /locus_tag="PK224_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB16151.1"
                     /translation="MRRNREQFVFGGAMVLILVMSLAPAFSQAAEWVHEGMAEEGKVT
                     MGFRVGPSFTTQSAGVSTVGPALNFQGMYGLNKWFRLGMMLEWESHGIDGPRSGSVDT
                     ITLLPVNLEYRPGHFGNFIPYFTTGLGVNINTKDVKDTFAWRIGGGIDYALTNLVQGA
                     PKGLMLNMEAAWKRNHPDSDGSTMGLLFGVRHTF"
     gene            complement(26689..27090)
                     /locus_tag="PK224_10210"
     CDS             complement(26689..27090)
                     /locus_tag="PK224_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB16152.1"
                     /translation="MSTRAVTVTMDDSLARARDLFNEHHFHHLLVVQGPTLLGIISDR
                     DLLKAVSPNIGTLSETDRDRATLNKRAHQIMSRKLITVAADTTVEAAARLLLEHRVSC
                     LPVVTTTGHLEGIITWRDLLREYLRHTPSGG"
     gene            27647..28330
                     /locus_tag="PK224_10215"
     CDS             27647..28330
                     /locus_tag="PK224_10215"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HRB16153.1"
                     /translation="MSKKEIDAARRLMSLMFKAHPWHGVSMGDHAPDLVTAYIEIVPT
                     DTVKYEVDKASGFLKVDRPQRFSNYCPSYYGLIPQTYCGERVAELFGARAGRPDMIGD
                     GDPLDICVLTEKTIPHSDILLTARPIGGFSMADGGEADDKIIAVMRDDAVYGSFTDLK
                     ECPAALLDRLHHYFLTYKHAPGSTQHKVEITSIYGRDEAMKVIHASHDDYKQKFPELE
                     FMWPASMNS"
     gene            28360..30090
                     /locus_tag="PK224_10220"
     CDS             28360..30090
                     /locus_tag="PK224_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB16154.1"
                     /translation="MKHTTTHSQTEPTAKGFSPGFAALGLEASLLTTLEALGYEEPTP
                     IQREAIPPLLEGRDLLGQAATGTGKTAAFALPLLQRIAHGPRQRPTALVLVPTRELAV
                     QVSEAVQRYGKELRIGVLALYGGQAMGPQLQALRRGVEVIVATPGRALDHLRRKTLKL
                     ADLQVVVLDEADEMLDMGFADDLDAILEQTPASKQTALFSATMPPRIASIARRHLKNP
                     VDVTIAREPVKAGAAPRVQQTAYVVARQHKVSALARVLDIATPKSALVFCRTRLEVDE
                     VTAALNGRGYRAEAIHGGMSQVQRDRVMQAFRSGQTELLVATDVAARGLDIPSVSHVI
                     NYDLPSSLEVYVHRIGRTGRAGREGAAMTIIEPREQRLLRAVEQHTKAKITVAAVPSL
                     GDLLAKRLERTKASIQETLEAGELEDFRRVVEALSASAEPADIAAAAIKLVYRSHGGE
                     RAEEEIPAVSTRAPEPYRPSRSSYGSAPQPGDRARAPRGGPSRGGRAAGTVRVYVGAG
                     RAAGIRPGDLVGAIANEAGVPSRMIGAIEVEERFSLVDVQEEAARQIIEALGRTRIKG
                     QKVAVRLFRD"
     gene            30540..32102
                     /locus_tag="PK224_10225"
     CDS             30540..32102
                     /locus_tag="PK224_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRB16155.1"
                     /translation="MAVTLAPWKAVLRDWQARAVADVLAQPREDYLVTATPAAGKTRF
                     ALRIAHQYLADRAASRVLVICPTNHLRTQWASAAGQVGIQLDPALSNEQACEARDYHG
                     AVVTYQQVCLAPEVFRRACRSRKTLVILDELHHAGDGKDWGKALRESFGEAVFRLALS
                     GTPFRSDNNPIPFVRYEQGESQADFTYGYSHSIRDGVCRPILFPSYEGELTWLSDGRE
                     HRATFEDGLTFERQRERLKTALLQETWLGPVLSDAHAELQRLRKQEQADAGGLIVTMN
                     QDHARQVADLVLKLTGTRALVAVSDDPSASKTIETFAHHKQQQWLVAVNMVSEGVDIP
                     RLRVGVYATNVLTEMYFRQVVGRFVRMQDKVPKPQRAWLYLPKDATLVHYAKRIKVER
                     DHVLEEIMPAVQRTLFGTAATSLKEYIPLNGVARLDALIGADEGDSAGDGKGQPEPAV
                     ALHDQKNTLRDKHRSLVGAVARKCGMDHRQLNAELIKRTGGRVDQATIPQLERRIALL
                     EKWRDSGFDKKR"
     gene            32185..32739
                     /locus_tag="PK224_10230"
     CDS             32185..32739
                     /locus_tag="PK224_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16156.1"
                     /translation="MAGKQVARRTSAVAKKITKQAVKKSGTKKAAPARRAKTPRAKRP
                     SVAVVVLSGSTPLRRNKAAELVAEELQLDLAKVNLASVVSRQVGETEKNINRVFDEAE
                     RSGSILFFDGADALFGTGRAGKAGTSHSTAAGAAHLLQRIEDHNGLVILTTNGKSRID
                     QALSRQAKVSVMVGIKKKPKKKTA"
     gene            complement(32914..33084)
                     /locus_tag="PK224_10235"
     CDS             complement(32914..33084)
                     /locus_tag="PK224_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16157.1"
                     /translation="MPKARTAKNCYYCEAEDRIKMTFMLCGLCHRHFCSAHGVPDLEQ
                     CTKCLEASEETE"
     gene            complement(33567..35189)
                     /locus_tag="PK224_10240"
     CDS             complement(33567..35189)
                     /locus_tag="PK224_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRB16158.1"
                     /translation="MSLPVEKSAADELRREVLRRRTFAIISHPDAGKTTLTEKLLLYA
                     GAVHLAGAVQARSSQRQAKSDWMELEQARGISITSTMLQFDYQGARVNLLDTPGHQDF
                     SEDTYRTLMAVDSAVMVLDGAKGIEPQTKKLFAVCRKRGIPILTFINKMDQPGRHPFD
                     LLDEIERTLGMTAVPFNWPIGEGSGFQGLYDFQHRQVLFFQRTAHNQRRAPMTVEAFP
                     NPKLAETLGDAYGPLQEEIGLLTGAGTSFDRDRFLAGELTPVFFGSALTNFGVGPFLD
                     AFTKLAPPPGPRQTAQGLIQPTDEAFSGFVFKIQANMDPQHRDRMAFLRICSGRFEKD
                     MMVHHARLGRKIRMTRPHRLFGRDRETIDEAYPGDVVGLVNPGLFTIGDTLTSDPALT
                     FDPIPHFAPECFGLLRTQDISKHKQFQKGLKQLEEEGVMQIFYSPDHVRREPVLAAVG
                     ELQFDVVMSRLENEYGVKTSVERLPHALARWIVGDPATISAVYWPSDTIRLVDQQGRG
                     VMLFTTEHLLAYCIKSNPSIKFLLREEVEQAQ"
     gene            complement(35977..37308)
                     /locus_tag="PK224_10245"
     CDS             complement(35977..37308)
                     /locus_tag="PK224_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRB16159.1"
                     /translation="MTKLARIPQFFKADLWTIDPASVPTLQRIGIHAMRLVIAVALEF
                     RHRLLDARAAGLVYTTLLSLVPFLAVMFSVLKAFGVHQQIEPVLAQALEPLGPNGQEI
                     TGKIIGFVDNLKIGVLGIVGVAGLFYTTYSLIDKIEEAFNAIWLVREGRSWGRKFADY
                     LSAVLVGPLLVVTAFGLLASVHSNTLVQRVLEIQPFGYLMLLAGRLLPFVILSGLFTF
                     LYKFIPHTQVRFDSALVGGITAAVLWGIAGEAFAMFVAQSAQYSAIYSGFAVLVLFLL
                     WLYVGWLIVLIGAQFSFFHQYPTAYVSRLLWQQGTHAFRERLALNLLVVLTRQYLKGE
                     GPMPAGDLAIELHLPVSLVAEQVRHLVETGLLGLMANPEGISLTKPPELISIKEVLDA
                     THQGRAADATVSLGTEDTVREVLRRRDTAIEQALAGQTLRSLVEIPMSRKT"
     gene            37577..39469
                     /locus_tag="PK224_10250"
     CDS             37577..39469
                     /locus_tag="PK224_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRB16160.1"
                     /translation="MTTTQEPPEDRFCDLVMKGGITSGVVYPKAIELLSHQYRFKSIG
                     GTSAGAIAAVVTAAAEYQRRWKGSRAGFDLLGTLPTELQRPVAPGTSKLLSLFQPQPA
                     MRRLFSVLIGALNSEKTSSRIAQIITGFLRAYWPATLAALVIALAVGAVGLGWFAACL
                     TLVMALPLLVGLWVYDDVTRRMVAHDLGLCTGLTEDGGNEALTPWLHALIQQVAGRSL
                     SDPPLTFGDLWNAPGFPPRWLNVPADPKPRSIDLQMFSTNLSHGRPYIFPLPERNPQP
                     TRFHDRDRLYFTEREMKRCLPVDVVAWMVEKSKPYTVEPGREEKDPPADTEKGMREVP
                     EPRDFPVLLAARMSLSFPFLFTAVPLHAIDHDPLRAREFRRCWFSDGGISSNFPMHLF
                     DGLVPMWPTFGLNLEPKIKGREMVFLPEQYDQGYGERWNHIPEKKGAGTLGGFISAIV
                     GTMQNWNDNSLARMPGVRDRVARVRLSKEEGGMNLNMEARLIKNIAERGVDATRELLE
                     RFSGPSPDGGAAKGWDEHRFVRLHVLIKMLAARSPGVVGALTSCGHATDFSTLLKNMM
                     NAKDKRGNPLPPPGYEEPMTKEQCQELEAFVTVLRDLATAMTTPARPIPFKPIPNPEL
                     RVRPPL"
     gene            complement(39562..39867)
                     /locus_tag="PK224_10255"
     CDS             complement(39562..39867)
                     /locus_tag="PK224_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16161.1"
                     /translation="MQKSLIVAIAVAAGLGASSVSSFLPSAHAEGLVAEGKEAMKGTG
                     KSLTEATGKLKTDATQTKEDTKKLDIDKTKQGVGQVKEDVKGVKEGAKGALTNPLGK"
     gene            40187..43312
                     /locus_tag="PK224_10260"
     CDS             40187..43312
                     /locus_tag="PK224_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HRB16162.1"
                     /translation="MQLPDGLYDKLVTESVAQLIAGLPDSFGHTCSILPLEEVSERIA
                     DALARQVTRLLDELGGQGAEKAKRQLDLVNALLVHLRQPAAGGVDPLVEPPQILRAIH
                     SIGAAPEAPATGLAIPWLFTAGKGSPSLLTELRREIACCDQVDILVSFITVAGVRKLF
                     DILQTATAVSAVGQPRTRLRILTTTYTGATEMEALDYFGRLPGCEIKVSLDGRRTRLH
                     AKAWIFHRKTRFGSAYVGSANLSGSALLGGLEWTVKFTEQGQAGLFARAKAHFETLWN
                     DGEFQQYDAANQEHRSELSRALKREAGDQICASTTFFDLQPKDYQKDMLEQLTLEREH
                     GRFRNLIVAATGTGKTVVAAFDYRAACQAMGGRPRLLFVAHRKEILMQARRTYREVLR
                     DHSFGEMLGGGSEPQSYDHIFATIDSVCARDLVARCGPDYWHTVVVDECHRLAADRFD
                     MFVKAVFPKVMLGLTATPERSDGKPIFPYFDNRPDGSPAVELRLWHALDLQLLSPFEY
                     YGCDDDTDFSEVPWDKPGEREVIDNLVTGNDTRARLVVNEWRRLTGNPRRSKALVFCV
                     SVAHAEFMARKFNEAGLPSLCIVGSTDADARRQGPERLVRGELCALVTCDLYNEGVDL
                     PSVDTLLLLRPTQSPVLFQQQIGRGLRLHEGKQSCLILDFVGRYREEFRFDRLLSAIT
                     GLSRGQLSEPVEKGFSSLSPGCLIQLQQFTKEQILRNLRTAMNQSWRRLRGELQSYAA
                     LRGRTAVNLGDFLREQAVQLHEIYRSHGRSGWTALRRDAGLVTTPESPADEYFGRRFS
                     DLLHCNDPEQTDLVVRISEPAVQYHTLNSRERCRLQMLAYQVDGQHHQAGTGEAFLAR
                     LSRAPEIYAELGELGGVLRAKGNLRFRPVPGLIDVPLCLHASYGIREILTAVGWLTAE
                     KRIPFQAGVLPLQNRKVELLFVTLDKSEGFHDRIAYRDYAISSERFHWQSQNSAGPDT
                     PAGRRYLESPRNGWSFQLFVRRSKGDSYRACGPVTIEKAEGEKPMSIEWKLGVLLPVH
                     LFQEFSILRGQ"
     gene            43840..44058
                     /locus_tag="PK224_10265"
     CDS             43840..44058
                     /locus_tag="PK224_10265"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HRB16163.1"
                     /translation="MTSPHPRDPLHGITLETIVTTLVERHGWAELGRRLPVRCFLNNP
                     SVKSSLTFLRKTPWARERVEGMYIAELP"
     gene            44455..46602
                     /locus_tag="PK224_10270"
     CDS             44455..46602
                     /locus_tag="PK224_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRB16164.1"
                     /translation="MNELQSKREAAEKELGEINSPPTLRKGAPPGTTDARLMERRSLL
                     QQLVQTYEQHLDALRKFDPMRLRVREVERQNKEWDGFSTPPPYSVLMVDELRDAARSM
                     ALTAQGIQTRLTMTESIADSTRRNLKASQEQARQASERLEGVQDPVKREALTWDRDLA
                     QLRERVESARTGMLEAEKAQIQEELTEAQQRITLLKRQRAVAAQDVEFTKQDYDKIKK
                     RLDADHQALMSELDRALLEQAAQRQAVAASEAKLAAADRKAPPSSKTSSRGLKEEGVS
                     HFTEAVELTRLQFDNANLRVDFLRQMVNGLERERQIWNIRYATAQGALSVVEERDASQ
                     KLTAAAKRIQGWKEYGLQQLGMVGSQISDVENRLEETSSSATGQYLNDMLRAYRHRED
                     LYRRMLQRTDSLLGLIEHRQTEFVQREQARSIVTRLKDWGTSSLAVVGNMWNVELFTA
                     EDTIEVDGKSVTGRRSVTVGKVLTAIALLVIGYWCAVILARFAEKQAISRLQVDPNVA
                     NIIRQWALAFLFLVLVSITLTFVKIPITAFAFLGGALAIGVGFGTQNLLKNVISGLLL
                     LMERPLRVGDVIEVDGIRGMVTTIGLRSSTIRDVNGVETLIPNSNLLERNLTNWTYSS
                     FRKRYSIRIAVATGSDARRVKEMLRDLAGQHGQILKEPEPYVLFEDFSEHALVFALHY
                     WIDIGPSIEPATVASDLRFMIENTFAEAKIVRK"
     gene            46661..47203
                     /locus_tag="PK224_10275"
     CDS             46661..47203
                     /locus_tag="PK224_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HRB16165.1"
                     /translation="MAPNATIFKATLHIADVDRHYYEDHTVTLARHPSETDERMMVRL
                     LAFAFHAHEALLFGRGLSTEDEPALWQKDLTGLIELWIEVGLPDEKALRQACGRAQRV
                     CVYTYGGRAADQWWKENLAVLERLKNLTVMNLSPEGSRALVGLAQRNMELHCTIQDGQ
                     MLIGDGTNAVQIELTTLKEV"
     gene            47437..47829
                     /locus_tag="PK224_10280"
     CDS             47437..47829
                     /locus_tag="PK224_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRB16166.1"
                     /translation="MKEKKRIPVDGEPVQWKSPFAALKSLELPPTPPRDKTERPAMAS
                     SSQAKNRGRVDIIRQTAHRGGKTVTVVTGFTGIGQDEKEQLAKEMQKACGVGGTVKEG
                     RIEIQGDKRDDVARILSKAGFRPVFAGG"
     gene            47836..48951
                     /locus_tag="PK224_10285"
     CDS             47836..48951
                     /locus_tag="PK224_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRB16167.1"
                     /translation="MAEPLKNSFGADVPRTIARMIAAVFPRFDQKAFVRSSLEGYDAL
                     ELMPRGWKIAHQLRRSLPDDYEKAVDILLASLDRKPERTVAQGMGGFLFLPHVFFVAE
                     YGLEHFEISMRAQYVLTQRFTAEFSIRRYLERHQVATLARLMEWSADSSEDVRRLVSE
                     GTRPRLPWASRLRAFQADPRPVLALLERLKDAPSFYVRRSVANNLNDIGKDHPALLVE
                     TAKRWMVGATEERRWIVRHALRSAVKRADADALAVLGYGESAFLSIRKGRVTPPRVTI
                     GSAVMIDCELVNRSAKPQRVLADLRVHYVKANGSKRPKVFKLKAVLLPARASLPLSKR
                     LLLSQLTTRTHYPGTHRVELLINGRSHPLGRFDLMEP"
     gene            49305..51257
                     /locus_tag="PK224_10290"
     CDS             49305..51257
                     /locus_tag="PK224_10290"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB16168.1"
                     /translation="MYTDFYGLIRAPFGTTPDADNLFLSPSHREALGSILYGIKERKG
                     VILVTGEVGVGKTTILRRYLQIAAPPKQSSPHPQKTVHLYNPNLTFDRLLTTILQQLG
                     AQPVSEEQAEMLAQLHDLAKAERNGGGTVVLVIDEAQCMPVETLEGIRMLTNLETAHE
                     KLIQIVLTGQPELEALLERHEVRHIRERIAVRARILPLTDTESVAYIQHRLDQASREQ
                     VSIFSPAALRILAKAAHGIPRRLNILCDNALMTGFRRREPIISRQVAQQVVAEIDEVQ
                     ARPVWKRREAVVLASVLALAFPVWLYLGWFPSGPWEGFGQTALEAPPSPKAIGLSGGL
                     PVSPRNAREVLPVDSVSTVATANASPMTLQHAAPLPVPRPVPVLPDLVRVSGPPKSSP
                     QNEALRVLDDKGIQTAMSGELVSPDDAESGEIARLLVVLLDAGRVVLGKAQATINNPR
                     LEDKGFSGVVFQARLRKEFLARTGHDLQALNAAAMPERAKPLLAKLAGIMQKAVQDVQ
                     SDINKKGIGFKGFIPATFGTQVAASFSKDTGLKLRQIGPPDVDPRNPDNKPDEQETEA
                     LLVVQKSHPRVGDHVISQRLADQSVRVVLPLFYTKACLSCHGKPKGELDISGYEKEGF
                     KEGDLGGAISVILPSAAPTTASQREG"
     gene            complement(51280..51888)
                     /locus_tag="PK224_10295"
     CDS             complement(51280..51888)
                     /locus_tag="PK224_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HRB16169.1"
                     /translation="MRVIVIGGTGTIGSAVVKLLSTRHDVVTVGHKRGTYQIDLASTD
                     SIASLFKTIGTCDAVVSTAGIAKFASLDDLTYDDYFIGLKNKLMGQANLVRIGRPFVT
                     NHGSFTLTSGVLSQEPMKGSCAISMANAGLEGFVRAAAIDLPRSLRVNVVSPPWVTET
                     LIARGMDPSIGLPAETVAQCYLASVEGTMTGQVIDPRKHAGA"
BASE COUNT        10184 a        14820 c        16411 g        10702 t
ORIGIN      
        1 gaagcgaggg cggcagtcgc gtaaccacgt gtccacgaaa tcgggggaag atcacgctgt
       61 attgtagaag aaggagtaag tatgtaccga gctcgacacc tcttcgacat ttgcattctt
      121 ctggtgggtt tgtttgcgag cggctgttcg tcgtctgcga cgtatatcag agcaacggtt
      181 cctgaccatc cgcttgaccg ggtcgcacaa caatcccaag aacagtggtc cgtcgagaag
      241 gtggatgcca atacgttaag tatgagcgac gggtggccgg catatagctt cggagcgctg
      301 ggttatggcg cgtctcatgc caacctgtct ttcgacccta ccacctccga actggatgtg
      361 cagtactact ttaaagcttt tccgatcatg acgctctgga ttccattttc aatcgatgcc
      421 gagccggggg cattcggggc agcgctgaaa ccgaccatga atgatcaaat taatgagatc
      481 atcaaatgga gcggtgggac gatcatctcc aggcgagcgg gggatcggtc agaaccattt
      541 cctcctaagt ccaaagggaa ggtttcttca ttatcgccgg cgacccccta agccgatcac
      601 cgactcagca cagagtggtg tgggaccccg tctcgattga cagcagtctt catcgatcgg
      661 taggctaagg aaaaacggga gggtttgtga tggcacttct tctcgcgttc tacctcatct
      721 tttccacagt cattcccgca tcagcgttag aggattctcc ggccaccgcc gccttggtca
      781 tcaaggccga tgtcttgtat tgggaagagg acgaactggt cgtgaaggaa ttctccggtc
      841 acgaagaacg cctgaaagtg acgcctgaga ccaaaatcga gggcgtggtg ggcggtcgct
      901 tgaagaccgg cgacaagatc gtggcacaga ttaccgatga ccgtaaggcg ctctccatca
      961 agttgcaggt tccggacggc cggtaggggc ctctggccag gctcacgctc actcagtgcc
     1021 gaccgtgagc cgcacctgca tctatattca ggcaaccatt tcggctttgc accgtcacaa
     1081 ttcggagctt catccgctct taccgttact tgaacatgtc gtagtcgtaa atcgagtggt
     1141 gatggccgat gtaccggagc tgcacctggt tcgggccgag gaatttaaag atgatgcgat
     1201 aggatcgatc gacacgaaag ctccacactt cgtgatgttt ggggcggagc ttctctgtat
     1261 ggagcgatgg gtaaaacgga ttctcccgga agagatccgt tttggaagcg gcctttcgtt
     1321 gaattggttt gggaagcttg gagaaaaggg cgtcgaaggt tgcggtagtg gaaagctcga
     1381 gcatgatcga actacgcgat caactctcgt agcgactttc gtttcgtgat tcgccccgct
     1441 ttgtggtcct tttcagaggt gtccagcgca tcgaggaatt ctcggcgata gaggccgatc
     1501 gaatcacaaa aagactcaaa attttctttt gagattttga actgaaaact gtctttggtt
     1561 cgtttcacca cttttgctgc aagcgccatg atgtccctcg ttatgcgcta attttaccgc
     1621 cttcagaggc gagagacaat ctgaaacacc gtggctctcg cttaatcgag gattgagaag
     1681 gcgccgataa aatacggtcc attcgccgcc tgcgcgcctc tgatccgcac ttcggtcaca
     1741 tcgaacaccc atccgccgac caaccaggga ttgggggcca ggttcgacca gtaccaccag
     1801 ctgccgttcg atttcctgaa ccagaattga atcggctcgg tgccggtcac tctgacgatc
     1861 ggcatgtcga acaccctggg ctgatgcgtg actcgatcct ccatccagat cttgatccag
     1921 tgatccttgc catgtgtgcc gagcgttctc ggttcatgaa tccaatcggc ggcgatgcag
     1981 tcgtcgccga cgttccgtcc aaagcccgcg ccggggccgt cgccgggatc gacaaaacag
     2041 ggggtgagca gactcggact gtcgaagacc aggtggatgt ggccataggt catgtcgtcc
     2101 acgttgatgt aattgccgaa gcagcgatgg cggtaggtcc agcaggcctg atgggtggca
     2161 ttacacggtt gcaactcgcc tgaattgtcg atgccggtag ggtatccgaa gttccctctt
     2221 gcctgcccgt agatataggt cagcgcaaac gtattatcga agtcgaccac gcagtcaccc
     2281 agccaatcgt gggcttcggc cggtgtggat gtcgctaaga gtccgcccag gatgacggtt
     2341 gtgaagagca gcagtgtgcc gagtggtatc cgcttcatgg tcctttcctt tctcgctcag
     2401 gtcgggtgcc gttcacggtc actttccggt cgcgtctcgt aaccgccgaa atgcctgatc
     2461 acgctcggct tccggcagaa gcagtgccgc atggtgatac cgatccacca aacgctgctt
     2521 ctgccgaaag tttgtggctt gggtaatctg tgtgagcaag ccgttcaaat cgactcgccc
     2581 ggggggattc tgctggtgca gcgacgattc cgccaacatg gcgcgcactt cctcatcgct
     2641 cgcttgccgt ggtccttgca cggtttgtcc ggcttcgccg agcggccctc ccggcacgaa
     2701 ctggtgagga ggaggcggga cacatccata gagaagcagc atcagcagag tgatccggcg
     2761 catcgttccc tccttgtctt ctgctgtcga tcctggaagc gtggccagaa agctcgctcc
     2821 gcaggaggtc tggtcacgac ttcacatcac gcaaactctg cgccatcgaa ctcagacaga
     2881 gcctctgcgc gtaataacaa tcgcttggaa gcggtgtctg gaactgctgg tatttgtggg
     2941 tggcagagga ggtatcgcat cggatacaga tccgtatccc atccgatacc actacgtagg
     3001 gttgttcacg atgacgtgaa ctaagaacag gaaaagatcc cgtcgattga aggagccggt
     3061 aggggcattc ggtccggagc ggccggcacg gattgacagg cagtcctgaa atacgtagcc
     3121 taggagacca ttctcccctg ccctccgaac cacggcagtc gctgttcgtc aggaggtcgt
     3181 catgctcgct tcgccgcttc gtgtcctggt ctcgatggcg attccggttt tcgcaggctt
     3241 gctggtctcc tgcgtgtcct tggaattcga ccgtatgaca ggaacggcat tccccacttc
     3301 ccagacggtc aatggccaga ccgtgacgtt gagctcgatc ttcgacgagg caggcatcaa
     3361 gttgacggtc gagcaggatg acacggggat tcagccgctc aacatcgccc aggacgagtg
     3421 catcagcgat gccgaactct cgacgttgga gaacggccat cgtcacctct cgctgtttcc
     3481 cacggccgcc tgtccctttg atttctgcaa tacctatcac ctctacggtg cggtggtgaa
     3541 tcactacggc gaggtgctcg atgtctgtat tcctgaattc atcctgggca aaatgtggca
     3601 gggacatacc aggtcggcct tcgccatttt ctatcgcatg aacaccatcc agaccgacgg
     3661 ggctgcctac ttccgcacga cggcccatga gatcggccat gccttcaatc tccaccacag
     3721 tgacggtgac ggcacctcga tcatgacgca atcagacgac ctgacgggcg atccggtgta
     3781 ccgcttttcc gagcaaagcc gggagcatct ggccaaccat ccggggcagt gcaaattccc
     3841 cggcgccatc ggagctgcgc cattcacgtg ggtcgccaac gaccatgcgg cctgggcgca
     3901 tcctctggaa gaattaacga tcaccgattg tcagtgaggc tgaagggcgg cttatgcacc
     3961 tcgatcctat cgcgcgccgg tccaggaagg tgtttctcgt ctgcgtggtc gcgctcctgt
     4021 tcccggcggt gcgggcaaac gacggcttgg cccaggaacc gcccggctcc accacgctcc
     4081 agctcacgct agacattgga caatccgcgc tgagtctcgg agagccggtc tatgccaccg
     4141 tccgcctcgt caatgtgggg acgatgccgg tggaggtgtt caaactgctc gacccgcaaa
     4201 ccggcgcgat ccagattgaa gtgtcgagct cgaataggcc acggttcgtg ttcctgccgt
     4261 tgttttacgc ggatgccgtg cacaacagga tcgctctcgc tccgggcgag gcagtcgtgg
     4321 cgatcgttcc tgtcttttat ggcgcgctgg gatggacgtt cgatcgacct gggacctatc
     4381 gcgtgacggc cgaataccgt cctcagggcg cgtctcaaca ggatcggata cgctcacacg
     4441 cggtcagcgt gacggtgacc gacgagcacg gcgccggggc ctccttactg accggctcgt
     4501 ccgcgggcga ggaagcggca aaatttctgt tatggcagcg gggcgaccaa ttgcaggcgg
     4561 gacaggcgct gttgaccggg ctgctgaccc aacatcccga gtcgccgctg gccgattatg
     4621 cgctgctggc gttcgggaga aatctgagcc ggagtttccg caattatgcc gttgggaaga
     4681 tccgtgaagc ggactgcggg tcggcgttga cctattttca gagaatccga tcagaccggc
     4741 tgccggtctt cttgcagatc caacaacgcc tggatgaagc ccgctgtttt atcaagttgt
     4801 cccagccggc taaggcgctc gattcgatgg agcgggccga acagttacgc gacggtcgtc
     4861 ctgaatacac gctcctcttt caacaagcga tacggttgga accgacgctg ggccttcact
     4921 ctgccatcaa cacaccgcac cgctaaatct gctctgtgaa cctcgatgac ggtcttttcc
     4981 ctgtcgacca ttatgcgctc gctccggttc tcgtcgcttc gacgaattgt tcggtggaat
     5041 cgtccaaggc ccggcctttg cgaccctggg cacgatggat gtacagcagg ccggcgctga
     5101 tgtcttgttt ctgtgcaggc gggagcggca tctggtacgc gacggcccgg gcatgatcgg
     5161 ccagttgaag cttcggatcg tacacgcgat tgaacttcca cagcatcttc acgaaatttg
     5221 tctggccgcg cagcagatgt ccggccgcga tacgcgccgt cgattttaag gcgttccatc
     5281 ccaggtgctt ctgattcagg atctgctgcg tcttgaccaa ttccgcataa aattccggca
     5341 gcggtaaggt ggtcggaagg acggcatgtt ggatgtcgta gaggcggtaa tcccgagtcg
     5401 tcacatgccg ggcttcggta tgccacgatt cggttccggg atagggcgtg ttcacgctga
     5461 tgttcacgat ctcggggatc tccaggcacc actgccggac gatctcgaag cgccgtcgat
     5521 cccaagcggg atcggcgatc aggttgatgg cgaccgttat accgagcgag cgggcgaatt
     5581 cgagcgcttc gaggttcttg ctgatcgtgg tgcgtttgcg atggtgcgcc agcccttctt
     5641 catccatcgc ctcgatgccg atgaacatgt aggcgaggcc cagccgcttc cacagctgaa
     5701 acacctcttt gttgcggagt agcacgtcgc ctctcgtctc caggtaatac tgcttcttga
     5761 ttcctcgtcg agacacggcc tctccgatcg ccatgccctg tttggcttga ataaaggcga
     5821 catcatcgac aatgaatacg ccgggctctc gaatggacgc cagatcgtcc acgatcttgt
     5881 cggggctggc agcgcgataa ctccgtccgt aaaaggtcca ggcgctgcag aaggagcagt
     5941 cccagggaca ccccctggtg aattcgatgg aggcgcaggg atcgagcacg ccgatgaaat
     6001 atttgcgccg gtgccgcagc aggtcgcgcg ccggatgaac ctcgttcagg ctggtgatca
     6061 tacgggcggg cggcccttca gcatagcgcg tgacggtgcc ggggacctct gcgagagccg
     6121 accggtcttc ttcgatggcc gttaacagcg gcccgatggc gctctcgcct tcgcccttga
     6181 ggacacagtc gatcgcgccg tccgcatgct caagcatggc ttgggccacg aacgatgcac
     6241 tgtgcccgcc gacgaagacc gcactgtgcg ggagttgttg tttcgtgagc ctcgcgagat
     6301 cgacgatttc gggcacgttg gccagatagt tacaggaaaa gccgatcgca tcgggacgac
     6361 gctcctgcag cagcgtgacg aacgacgcgt gcgactccac ttgcagatcg atgagcgtga
     6421 cgtcgtgccc ggcccggcgc gcggcggctg cgacgagctc aagccccagc ggttccaggc
     6481 gcagataaat tttcgtatac atcaatgggc ttggatggac gagcagcacg ttcatacctt
     6541 acctccctcg atggtggtgt ggtggctgac tataggatgt ctgtgacatg ttgaggacta
     6601 gggagagtcc tcgttctccc gtggtccgcc cagggggagg acagaagtcc gggctaggaa
     6661 ccggtccggc ttcggagtat tctcacccgg atatgtctgc tcaacgcctc aataggatgt
     6721 cggtccggtc cgtgaccggc tggtgggcgg ttgcctgtct cgtcttcgct ctgtcgggcc
     6781 tgcctgtttc cgtgggggca gccgtcacga cgttcatcgc gggagactat gcctttaccg
     6841 gccctgagca gctcgactcc ggccggcaaa cggtgcgatt gatcaatcgc ggccgggatg
     6901 tgcaccaggt tcaatttttg gcgctgccac cgggaaaaac cgtggtcgat gtcgaacggg
     6961 cattggcgtc ccggtcgcca agcttgccga attggctgcg gcgtcatggg ggagtgaata
     7021 gtgtcgctcc cggcgatgag gcgtccgtca gcattcagct tgatcccgga gactatctgt
     7081 tactctgcgg cattccggat gtagcgggcc gtccccacgc catgcgcggc atggtgcgtg
     7141 cgctgcgagt ggtggaggcc gcgccttccg gcgaaccggc gccgcgggcc gatgcgacgc
     7201 tgcgtctgaa cgattttgcg tttgcgttga gtggcccgct gcaggcgggg tctcgaaccg
     7261 tgcatctcgt gaatgatggc ccgcaggctc atgaggtggt gctgattcgt ctcgcagagg
     7321 gagcgtcggc gaaggagttc atcgcgcggt acaggcctgg aggactgccc aattccgctg
     7381 ggcgggaagc cggcggcctc accggtctcg atccgggacg agagggttat gttcatctcg
     7441 aactggagcc ggggcgatac ggtctgctct gttttcttgc cgacccggtg accggagccc
     7501 ctcacttctc ccgcggcatg tggatggaca ttgaggtgaa gcaggcgcta cccgccgcgg
     7561 agggtccgtg acgtatcggg acttgatgcg gctggcgaac ggctatgcgg agtcgaaagt
     7621 gctgctcgtc gccaatgaac tcggcctgtt cacggcaatc ggccagggcg gcaagtgcgc
     7681 cgactcactg gccaccacct gcggcgcttc gcgcgagggc atggacctcc tgctgcaggc
     7741 gctcgcaggg cttggactgg tgcgtctgaa agcggggcgt tattggaata cgacgtttgg
     7801 gctgaactat ctggatggcc gaagcccgac ggcggtgacc aatctgctct ggctgctgaa
     7861 ccaccattgg tcggattgga cggggatggc gcgcgccatc cgccggggtc gtcccggctg
     7921 ggtaccggtc acaaagaccg cggcgttccg gcgacggttt gccctagcga tgcacgagcg
     7981 tagttatgtg ctggcgccgc tcacgctcga caggatccgg ctgccgcgtg gagctaggcg
     8041 cctgttggat gtaggcggcg ggtccggggc ctacagcatt gctctggcgc aacgtcatcc
     8101 cgatgttcat gtgctgctgg tcgatcaggc cgtgtcggtc gcgcgacggc agatcaaaga
     8161 ggctgggctg gcggaccgta tcgcggtgcg gcaggggaac gtgttgactg ccccgctcga
     8221 ctcgggattc gatctggtat tgatctccaa tctgctgcat gatttcagcg aggcggaaaa
     8281 tcgaggtctg ttgagacggg cgcatggcgc gctacgaccc gggggaaaga ttgtgatcgt
     8341 ggagtatttt ctcaacgctg ccggcaccct ccctgccgag gcggcggtat tttcgttgct
     8401 gatgtacgcg ttcacgccga ccgggcggag ttatgcctgg aacgacgtcg aggcctggct
     8461 gaggggtacc ggattcggac ggtttcgtcg gcagttggtg accgacagta tcggcacgct
     8521 ggaggccgtc aggctgtgac ttccgttggt gccggggcga cggcactcag ccctgcatga
     8581 tacctctgga ggtgccgccg gaccgggatg agcaatcgtg gcgggatgtg agggcagact
     8641 tcgcgatcga atgacacggc tcgttgatat cgcttcactc ccagtgccgc ttcagccagt
     8701 gcccagtaga gattctcaac ccaccctgac cggatcacgg tgtccagata tttgtacagg
     8761 acttgaggcg atcgtacggc gcctcgatag gcatctttga tttcgttcaa cagccgctct
     8821 tccttcgccg tccgctgata caggccttcc accacatggt tgagcgagag gaagtcttgc
     8881 agatcgaagc gggcatcgac aggttgtgtc tgctcacggt agaggtccca atcggtcgtg
     8941 aagtggagcc gctccagcag gggcagcagg tcctgcaacg tgatttcctc tgccgtttcg
     9001 caggagcggg agaggcggac cttgatgaag gtccgccgat cgtacgtgtg ctcgggcaaa
     9061 tagtgtgtgc cggagagttc cgccaatcga cccgcctggt cgcgtttcgg gaattgccat
     9121 tgccattggt ccgggaagtg aaccccggcc gcctgtaatt ccgccctgta gcagggaagc
     9181 aggtggtcga agaaggggcc gcctgcgtcg tccagcggcg ttcccggcag actgcccact
     9241 gccagagtga gtctggcgaa gggcatgcgg gtatagccat cgtcgtccac gacaaagttg
     9301 aatccgcccc acggtccgat gtccagcagg gtttcatggc cctgttccgc cagccaggtt
     9361 tcggtgtcga atgccagacg ccgccagagt tggatcaagg tgccgatgtg gacgtggcga
     9421 ttgcggaaca gcgggacaga gtcgggataa tagccgttcg tgctgcgaag gcgagtctgg
     9481 agtttgaggg tttcccactc gaccgcttga gccaaggttc ccttgtggac ggacagtccg
     9541 gagagatcgc gccggcaatc cgcggcgatt ccggaccaaa ggtccgctcc gggcctggcc
     9601 gaggccgcct gttgcgtgac gtgatcgatc aggttggaga tccccgacgg ggcatgagcc
     9661 gctgccagat tttgaaggga agttcggaag cggctgatgg cccggccgga gcggcgcgtc
     9721 caagcggcgc tgaacgcgct catgtcggag gccctgctcg tacaggcatc acacatcaca
     9781 aggactgagc tcccgttgct ccgccaggat gtgcgaggcc cttcctgcag cggggaatca
     9841 tgtgcccgcc ctttcatacg gtactcgggc tatgaggcgg aactcgtaaa ccccgaaggt
     9901 ctgggcaaga agattacgag gggactgaga atcgtgagga tttgggaagg tagaaggttg
     9961 cgatcaccag acaagcgccg gctctgaatg cggcgaatcc cggtcagcga atggggatga
    10021 gcggtgcgtg aagcgatgcc cgagagggta ggcggtgggc gcctcgactg aaccagcttg
    10081 tgcctggcgg cggtggaagt gcggcgttag cgccgtctcc gcactcgcac gggctgccgc
    10141 cgctggatcc agtcccagat gagggtgccg atgaccagcg cgggaagcgc caacagccag
    10201 acataccagc ttgtccagag cgcggggtcg tgcatgatcg ttcctccagt gtgtccggca
    10261 gggaatagag caagcaccat accgtcgtgc attgttccga gggattgcgc cgcaaaccgt
    10321 gaaacctccg gcgattcgca tagatgcaag gttatggcgg cgatgtttcc tgtcgcaacg
    10381 cgcgccaggc aaattttgcc gtcgcataaa cctcacaata cgtgacgcca ttcccacagg
    10441 tgccggatgg agacaacctg gggaggtcga gggagccggt gcatgggagc agggattcgg
    10501 ctcgtttatc ttgccagcaa gaagacgagt tgcggcagcc agggcagcaa ggccacgccg
    10561 acgcagatcg agagggcgac aatcgaggtg acgaggtctt cgtccaatcc cgtgtcggtt
    10621 gcgaaaatga ccgccgtcac ggcggacggc atggcggaga caaggatgac gatcgcccgt
    10681 tccatgcctg caaggtcgag cccataggca accgcaaacc cgaggagcaa tccgccgacc
    10741 atccgcatgc ccaccccaag caatgccaag gcccaggttc gacgtacggc cgagaaactg
    10801 atcgacaggc ccagcaccag actcgccagg ggtgtcgtgg tgaggtggat gggggtgagt
    10861 gcatcgtgga gccagccggg aagatggagt ccaagcaggg tgagcagcag gctgaggcag
    10921 agggcccaga gcggcggaga cgacacaaac cgccgcatgg ctgatcgggc ggtggtgccc
    10981 ttcgccccgt gccagaccgc cagcccataa atcaccgtga gtgtcagcgt cgtctgcccc
    11041 aggtcgaaca ggatcgcctg cgccagtccc ttgtcaccga gggttgcgag gacgacggga
    11101 taggcgaaat agacggagtt gatacagccg gtgccgagga gaaatccccc tcgtgtggcg
    11161 cggggtagat gcagcaggcg ggcgaccggc caggagcaga acagcatcga gagggtcacc
    11221 aggcaggcgg ccaacggtaa cttccaggct gtgggggcaa agaccactcg atccagtgac
    11281 accaatatcg tggccggcag gctgatcgaa aagacgacaa aggccagacg ttccgcatgg
    11341 attctcccga gcaggccgga tgaccggagc agcgcaccga gcaggaagat ggcgatgaag
    11401 gcttggaggg tggcgggcat cgacgggagg gtagcagaaa acccgttcga agaggaggtg
    11461 cgtcgtccga ctgatccccg cgtctttttc tatgcgcctg aacggcgggc ctgctaccat
    11521 ggcccatccc atgacgcacc cggagagctc aacacaaccg cggcaagcgg cggtcttctt
    11581 tatccttgtc acggtcgtgc tcgacatgtt gtcgttcggg atcatcattc ccgtcctgcc
    11641 gaaactggtc gaagagtttc tcggcggcga taccgcgcag gccgcggaaa tctacgggct
    11701 gatgggcacg tcctgggcct tgatgcagtt cgtctgttcg ccgattcaag gcgcgctgtc
    11761 cgatcgcttc gggcggcgtc cggtcgtgct tctctccaac ctagggctcg gtctcgattt
    11821 cattctcatg gcgctggcgc ccagtctcgc ctggctcttt gcaggccggg tgatctccgg
    11881 catcgcctca tcgagtttca gcacggccgg tgcctacatc gccgatgtga cgccgccgga
    11941 caaacgcgcg gctgcattcg gcatgatggg ggcctcgttc ggtctcgggt ttgtgttggg
    12001 tcctgccgtg ggtgggctgc tgggcgccgt cgatccccgc tggccctttt ggggcgcggc
    12061 ggcgacgagt ctgctcaacg cctgttacgg gttctttgtg ctcccggaat cgctcccgat
    12121 ggagaagcgg gcgccgttcc gctggaaacg ggccaatccg gccggcgcgt tgatgctgtt
    12181 gcgctcacat catgagctgt tcggtctggc gacggcgaac tttctgatga acctggccca
    12241 cggggtgttg cccagtgtag cggtgctgta cctggggtat cggtatggct gggggccatc
    12301 ggccgtgggg ttcacgctcg cagcggtcgg ggtctgcgcc atggttgtgc agggcacgct
    12361 ggtgaggccg atcacggcgc ggctgggaga gcggcgcacg ttgatcaccg ggctgctgtg
    12421 cggggccacc ggtttcgcca tctatggact cgcgcctaca ccgctcatct attgcttagg
    12481 cattccagtg atggcgtttt ggggactcgc cggtccgtcc gcgcaaatgt ttatgacgcg
    12541 ccgcgtgagt gcgtccgagc agggacaatt acaaggcgcc attgccagct tgaccggcat
    12601 cgccggcttg atcggcccca cgctcttcac gcagaccttc gccgcgttca tcggcccgca
    12661 ggccgactgg cacctgccgg gggcgcccta tctgttatcc acgacgctgt tgctgatcgg
    12721 ggcaggtatc gcctggcggg cgaccagagc cgtctgacgg gctatcggtt cccgtgcacg
    12781 gacaactcgc ggttgcggta gattgccagc gcatgggtgt ccgcatcctg agactccgtg
    12841 gcttgcccca gttgcccata gagatcggcc aggagcgacc aggcttccgc ctcggcggcg
    12901 gtgtcgtgga ccgcttgcgc aatctgcagg gcgcgtttcc cttgggcgac gcgatccttc
    12961 agggaggcct ggtctccatc cagccgcgcg agccgcagtt gcatcatgcc ttcgaacggc
    13021 aggtgatgtt ccgcttccgc caggctgagg gcgtcgagat aggcgctgtg cgcgtcactg
    13081 gtgtggccga gcagccaatg ggcatctccg agcgcgaccg tggcggtgaa ctgttggtcc
    13141 acgtgccgtc tcgtacgggc ctcttccgag gcctgctgca aggtggtcag ccccgcgctg
    13201 ttctctccct gtgcgatctg tagcaacccc aactgcacca tcgtgtcgtt ccatccccgg
    13261 gcatttctca cgcgcgtgaa gagggcggcg gcctgggtga gggcgtcgcg cgattcgtcc
    13321 gatagcccca actgcgccga gactcgaccg attgcgagca gcgactcggc atgggcggat
    13381 cgatccgacg ccttggcgaa cagcgacacc gcttccttga gtgaggtgtg ggcctgctgc
    13441 aggtgaccct ggcgttcatc gatccgcgca atcttcacca gcgtggtggc gatgccttgg
    13501 ggctgtctct tctcccggaa tttcgacaag gccaggttgt aatacgtcag cgactccggg
    13561 aaatgttcct gctgatcatg gattgtcccg atgcgaagca gttcatggcc tgacagggta
    13621 ttgggagatg cggccggtgc ggccggttct gcggagaacg cctggtccgc acattggccg
    13681 gacaggcccc agagcaggag aagcgagcag ctgatcacga tgtggcgaat caggcctggt
    13741 ttcgaacggt gatggagtgc tgaccggtga tgcatggcct aaacatgcac gtgccggttg
    13801 ccaaggtcaa gtcggtgcgg ttgcatgggc ccggcgcttg cctcgcctgt tccggagggg
    13861 taagatcggt catcccgcca tacagagagg aggtgccatg tctacgacac gcgcgactcc
    13921 caaacgagcc cggatctccc tcgaccgcag catcgagcgt ttacgcaagc agttggccga
    13981 tctggccgcc tatcttggca ggggccttcc cacccgcagc ctggaagagt tcgatcagga
    14041 caccgaaaac ctcattgccg aattgctcgg ggatacctcc gagctgcttg aggcgtatga
    14101 atatgccgag ttcggcgaag cggccggctt ggtgaatctg acagatgaag caccggaaag
    14161 cgccggtgtg gagagtcagc gacagcgcct cttgcaacgc agtcgggtgc tggaaagttg
    14221 cattgccgaa ctcgaagccc gacgcgctgc cgaaccgaaa cagtcacaag ccagccggca
    14281 gaccctcatc ggtcctcagg tcgccgagca tatgtcgtcc gagatccgta gcctgtccca
    14341 ggatgccagc ctcaaggacg ctggtcaggc catggagaaa tggaaactgg gctcgctgtt
    14401 gctgaccgac aagcaggcct atgtcgggtt catcaccgat tcagacctcg cccgcgcagt
    14461 ggtcgccagg ggggtggatc ctgcgacggg ggtcaacgtg tatatgcgaa ggccggtggt
    14521 gtcgatcgcc ggcgaccgtc cgatcatcga ggcggtgcgc atgatgaagg atcaggccac
    14581 gcggcatctg gcggtcacgc aggatggctc gattatcggc gtgatttctg tgtccaacat
    14641 tctgcggtac tactcgggcg tggtgtagcc ggacagtgtg accatcacgg gatggtcaaa
    14701 aaggctgtca gcaaggccgc agcgagtgag gggccgaggc gtaccctcaa gggtacgttg
    14761 agggtccgag cgatgcgaga acgcagctgg cgggcttttt caacatccca ggcaaaagga
    14821 ggcgggatat gggcccgacg aaagcaatcg ttcaaggaca gtcgctccac gaagccgtga
    14881 gcggcaaact gatacgggac ggatttacga gccagcaggc gatggaagat tacgtgcggc
    14941 agcactatat cgtcatgccg gtacgcgaca atgccggaca gccctggcag ttggacggga
    15001 aaccggtcta ctgtctgcac ggtgtgcaat acgaaacggt cgatgaccag aaggtgcatc
    15061 tttcccgatg cccggattgc ggcggcatgg ggatcagggc ggatgaactc acggtcgatt
    15121 ccgactgtat tcgctgcacc gcctgtggcc atgagtttga tgcgcggttg gagatgatgg
    15181 agacatagga gaggggcctg cctgctcagt gtcttttgct cgcgcaacgc gcggccttag
    15241 aaggccctcg ttggacgcgc gcagtgagac accgagcagg caggccccag acagaaaaga
    15301 gagggctatc agcgatacgc tcttattccc cgcgagatac gtttcacgtc ttacgtgtga
    15361 cgcttcacgc tcgacgcttc gcgattgatc tgccttccgg aggagtaaga agaaattcag
    15421 cgacaggaat gagaaccggt tatactcagc gcctgctcct cataagagga ggccacgtgg
    15481 gtcatactag aatcggaaag ggacgaagga catggcaggt gcaacggtga ctcggccacg
    15541 caagaaggct ccgcagcaga acaagaaggc gcctgcgaga aaacccaaga aggctcgtgt
    15601 gcaggagccg tccggtgcgg tgatcgtgct gtccggagcc acgccgcttc gccggcagaa
    15661 ggccgcggag gtattggccg aagaactgca attggacctg gccagcgtgg atttgtcggc
    15721 ggtggtgagc aaacacgccg gcgaaactga aaaaaatgtc aatcgcgtgt tcgacatggc
    15781 aaaccgccat gggtcgattc tttttttcga tgaggccgac gcgctctttg gcaagcgcac
    15841 ccaggtgcat ggcgcgcacg atcggtacgt aaatgccgag gtatcccatc tgattcagtg
    15901 catcgaagac caccagggcc ttgtcatcct gacgagtaac ggaaagagcc gcattgaaga
    15961 agccttctcg cccgagaagc cggtgattgt gctgggcgga gcctcaaaga aaaagaccgc
    16021 aaccaagcca aagccggcgg ctaagtccgc acccatgccc aagaagaagg tggcaaagaa
    16081 ggcgtgagca ggggcacggt gtctgcaggg atggtactcc cattgcgatc accgcgtgag
    16141 gggcttgggg gttacgtcat cctccctcgg cttatcgata aggtgcggct ccatgcgttg
    16201 ggcgcgttgc catcggacta tgtgcggaat ttattgaagc cagggcttac ccttgatggc
    16261 aggttcctga aattcaccgg actcgctgcc gacgcgctgc aaggcgcaat attgtcatcg
    16321 ccaacagacg aactcgtctt ggcgtgggtc gatcaacatg cggcaaacca ttcagatgcc
    16381 gagaaacagg catggtcaga gaaggcggct gcttttcgtc cggatcctcg cgtggctaaa
    16441 tttttgatgg cgagcctccc tgctctcgca gccagcgtgg atatcactgc ttataccctc
    16501 tttgatttga ttgatatgga cgaagggcgc ctaccgattc cgccgaatcc ggcgacctga
    16561 agtgggcgga ccggtcagag ttggccgcct cgcttcatca cggtagggat gcaatgaggg
    16621 ccattttcgt ggcccgatcc agtgacttga tggcggcttc gcgttgtaag gcggctccct
    16681 tgctgtcgag tgattcgata taccggaccg tgaacggtcc gcggcgtttc gtgtattttg
    16741 cccccttccc ggtcgcatgc gccttcatgc gccgttccaa atcattcgtg atcccggtgt
    16801 agagactgcc gtcgatacac tccaggatgt agacaatcca gatcatgtgg tgtgacgccg
    16861 gggtctccgt gcccagggaa ggtccttcgc ctggctggct catgatagcg aaaaagatcc
    16921 ggcgtggcga ctatttgggg aaagcgtggg ggaagtcgag caccagtctt gaaaggatgg
    16981 ccgccaagac gagggctgag gccagtgcca cgatcaggaa cttgcggtct ttgcgcaccg
    17041 aatcgtggaa tcgatatctg ctggatctgt gtagagactg acttgccacg cggccgtacc
    17101 tctggagtct atgaaatggc ccgtgcacta tgcctggctg atgcataggc tggaataggg
    17161 gttttcctga aaggctctgg aattattact gtctcgaagt tattcggtcc tgttcactgt
    17221 tctgcatact cgtccgatga gtccccggta tcgagctgag ttcctgccga tgtggccctg
    17281 attgacagct cagtcggcgg ccacgtagcg tacccattca tgtctgaggc aacgatgatc
    17341 gatcgagaac tcgccgccgc tgaagttgcg tggacacaag gcaatgcagg caaggcgcgg
    17401 gtctgcgcgc ggcgggctgt ggcgctggcc gccgcagcct ggtgggcgcg ctctggccgc
    17461 tcactccggc acggtgatgc cctggagcat ttgcgccgga ttcagcagca ggaggccttc
    17521 ccatcgcctg tccaacaggc ggcggagcga ttgaccaccg ccgtaaccaa gcggcatgac
    17581 gcgcccttta cgagtgatcc gatcggggac gcccggttga ttgtgggcta ccttgccgcg
    17641 gctacaacgg accggcggct tgcttgacgg ttggccccgg cgggtggaac agcgcctatc
    17701 tcgtccagcc gcaggtcgaa cgaagtggag ggttgtggtc attctggtca tgggcgtatc
    17761 gggagcgggg aagacgacga tcggccgtcg cttggcggac gaattgggct ggcagttttc
    17821 cgacggcgat gacttccatc cggcagcgaa tctcgagaag atgcggaatg gccatgcgct
    17881 gacggatgtt gatcgtcagc cctggctgga gcggatgcat gctgcgatcg tcgatcggat
    17941 cagtcggaac cagccggcgg tgcttgcctg ttccgtcttg aaggcgagtt accgcgcgat
    18001 cgtggaggag ggctgtcgcg cacatttgcg actggtctac ctgaaaggta gcttcgattt
    18061 attccatcaa cgcctgatcc accgccggga tcatttcatg ccccgggaat tgttggccag
    18121 tcagtttgcc gtccttgaag agccggccga tgccctggtg atcgacgcgg ctctcccgcc
    18181 gaacgagatc atccggcaga tccgtctcgg tattccggtt gacgcggcga aacactgagg
    18241 acggatcagg ctgcgaggcc ggaaatcatg tgtcccgttc cgacaacgcg atcgccacgt
    18301 ctgtctcaat ggcaaatcgg ccgcgcccgc gtggaaccag cctgatgtcc ggacatttct
    18361 gttccatccg tttgcgcagc agaatcagcc gacattccag gttgtcttta atctcgggga
    18421 gcccgagcca ggagtccatc cggagttctc ggttcgagaa ttccgttcga ccctcgtcgc
    18481 gatgccgtgt cagcaggcgc cagaggatgc gacccggcac gtttcgaatc aggtactcgc
    18541 cgtccacaaa aacacagtca tcctggggga agaaacagaa gctgcggctc cgcggtggcg
    18601 cggcgagcgc gcgcgaagac tgaggtcttg cgagggcgcc ttgtggagat tcttccgtat
    18661 cccggctgag ttgttcgatg cagcccccca gatgtgaggc gacaatcgtc aggagtgtgt
    18721 cctcccgaga catgaacgca aggggttctg cgctttcgac ggcgagcacg ccgatcaggc
    18781 ggcccctggt caggagcggg acggcgatct ggcctgccgc acttgggagg ccggggagtg
    18841 ggatttcccg gcagaccgcg tccactcccc cgcccgcctc cgcgcagtcc cgcaccgcgc
    18901 gggcgtaccg caggctgtga tcgatcgccg agagttggag cgcccgtctc gctcgagcca
    18961 ccgtgccgat caggccttcg ccgattccga cttcggagcc cactccattt tcagggtagc
    19021 cgcggctggc gatcgtgaac agcttcgtgt tgcgttcgtc caccagcaaa atcatcgagt
    19081 ggcggaaacc aaacaggtcg tcgagcaagc gcaaggaggt ttccagcagg tcccccagta
    19141 gttcggcccg gtgtatgcgc tcggagatga gccgcaatga ttccagatcc tgatccacga
    19201 acaggcgtga tggccgatcg gctgggatgg ccggcggcag ccgggtaaat ccgtccaccc
    19261 gttcgatacg taccacctca tacacgtcgg cggcgcgcag gcggaacacg tcggtcattc
    19321 cggcgtggga tgcgatcgct tgcagttgca gcgacatcga gtcgaagagc ggccctgagg
    19381 attcggagtg ctggtagcgg agataaatgc ggtgggcctg cagcgttctg ggatccagca
    19441 tcatggcgca ggccagcgga ttctcccgca cgttgcgatg ggtcttgttg aaaaattgat
    19501 gggagatcgc cacgtgcgtg ggatcgacgt aatggatctg gctgatgaac gtgatgttcg
    19561 gtgtgccgtc ttccgaacag gtgctcaagg cggcggggat gatgccttga aagcagggcc
    19621 agagttcttc caattgcgtc aggcggtctt ccacggctca gaccttttct ccggcgccgg
    19681 ggcccggcgt ctgatgaaat acgtcggtga cgtcgatgtc gatggccaag gcgggcgagg
    19741 tgatccaggc ttcgcacaag tcggccggta caccgatggc gacgagttct tcgacaaatt
    19801 cgcggtgcca gtcgcggctg ctctcgcgat cacgctgatc ggccggccgg atcgtccgga
    19861 cccgtccttt cagttggcag gccacatgat cggtcggcct agtgcaactg atcgccacga
    19921 gtccgttctc ttttagattt tcgatggttt tgcgggaaat cgcttccgtg agaaaaattg
    19981 tgaccgagcg gcgatccttc ccggcacgga ttccccaggc tctggtggac tccggcatca
    20041 gcgtcgagtc gcgtgtgccc acaatgacgg agaccccggt ttgaaggagg tcgaagaccg
    20101 gctgaggaat catgaggcgt ggccaggtgt cgaacttagg aaataattag tgaacgatag
    20161 catattctta ggaaccgccg ctcggcctgt gatggtatgt ttcgccctgc cgaaagctca
    20221 tggctgcacc gcgtaacgat tcgctggatg attgagatcg gctctgccga tttatatgga
    20281 aggaggttcc catgttgccc acgtcgtctc gatggctcac cccgctgctt gttctgcttc
    20341 tcgcccctgc cgtggttatg gccgatggac ctcgagcctt cgccggatcc cgcgccgccg
    20401 gtgcccccgt cgtgttcgtg gccaacgact atgggtttac cggcccggat cgcattccct
    20461 cgggagtcac cacgatgcag atcgtcaaca aggggcagga tctccaccac attcaactgc
    20521 tccggttgct tcagggcaaa acggcggcag actttcgcgc ggctctcaag gctgagcctg
    20581 ggcgtttgcc gaaatggatc gcgttcgtgg gcggcccgaa cgccgttctg ccgggcagtg
    20641 aggctctggc cacgatgagt ctcggggaag gtgaatacct gctgatttgt ctcatcccca
    20701 acaaggaggg agtaccacat atggctctgg ggatgcagaa gccgctgtcc gtccaaggcc
    20761 tgaagtccac cctggtttcc gagccgaacg ctgggctgac gatcacgcaa gccgactttc
    20821 gttttgccct gtcccgaccg gtgacagccg gctcgcacat gatccaggtc atgaatcacg
    20881 ggacgcaacc gcacgaggtc gtcgtattga agctcgatcc cggagcctcg gcaaaagatt
    20941 ttgcagcagc gtttgaaccc ggcgcctcag ggcctcctcc gggcaaaccg gttggcggag
    21001 tggtcggact cgaaacaggg gcgcatgcat ttttcaccgc gcgattcgag ccgggtcgtt
    21061 atggcctgat ctgctttttc cctgacccag ctacaggcaa accccatttt gctcacggaa
    21121 tgaccgcgga tttcactgtg gagtaacggc gtccgcacgc ttcggcaggc tcggctcccg
    21181 tgacgcggga gccgagctcg cagtccttgc tgaaactgcg gttccaagcc aggccccggt
    21241 cattcctgga cataatgcgt gcgcaccgcc tgaatctgcc cttctccatt gaccgtcatc
    21301 atttccgccg ccagtaaccc tttgacgcta cggtagtaca gcaacaggct gtcgacccct
    21361 gtcagcgtgc gcagcaattc aaagtgcagg tctggatagg cttggagtcc tttccggaaa
    21421 tactccttca atggctcgcg gccacggatg gttccggacg gctcaccgga tagcgccggc
    21481 acaaacggac tggtaaactc cacgtccgtc gcgtaatggt gcacaatggc gtccaggtcg
    21541 tgccggttcc aggcttcgat ccaggcccgg gccaggctgt tggcctgtcg gtcgttcata
    21601 tcgatcgccc ctttcgtaaa aacatgatgc ggctctacct agcctaggcg acatgggtgt
    21661 ggtgggcaag gggttgttga ggcttactgg aagatgatgc ggttcgatcc ggttgtaccg
    21721 gagtggcgga aaacggcatg gctccgttca tgacgcaggc ctcgccccgg aacggcctgg
    21781 cgctgatcgc cctgttcaca ttggtcaagg gcgtggtcct attgcttgtc ggcgccgggc
    21841 tgctccgatt ggtggatcct gagatcgcca ctttcctggc tccggctgtg gatctcttgc
    21901 atctgcacgg gcattcccgc ctgctccatt cgctgctgct caaggtcggt gtgctcccct
    21961 cccataccct gtttctgatg gcctatgcca gcctgctcta ttcagtcttc ctgctcattg
    22021 aaggctgcgg cctgtggctc gaagcttcct gggccggaca catggccgtg atttccacca
    22081 cggtgtttct gcccgtcgaa ttctacgacg tgctgaagca gatctcggtc gtgcatgtga
    22141 ccatgctcct tctcaatgtc gccatcgtgg ggtatctggt cgcgcaactg gagcgacggt
    22201 cgttgcggta agaccgaagc ctcgctccag gttgatgcga gccccggtga ccgtccaggg
    22261 gaatgcctag ctgccgacct gctcgaaggg aggtacaaaa gaatcaaact gggaggagac
    22321 gtcatgactg aagtcaattc catgtatcgt gcgcagacgc ggagtgatga cctgaaggcc
    22381 cagatcgaaa agctcgaagg cctcgcccac catacgtccg tgaaccccac gttcgagcgg
    22441 ttcgatgcgg aaacggaaga catcttgatg aaaatatatg gcgcgaacca taagtacgtg
    22501 gagtcgtaca aatacgccac ggtgggggaa gccgaagcgc tggtgaatct gcccgaatca
    22561 gcccaggaac cgcagacgcg agatattccg aagaaggggc tgcagcaacg ccggcaggtg
    22621 ttgcagagca ttctgaccga tttgcagaga ctcgaagtgc aagaagccga attgctcacc
    22681 ggagaagatc gcgaagatcc ccccggtccc agctagcccc gctgtactcc ggaattgtcc
    22741 aggacatgga ggtggtggcc gccgttccat ttatatccaa tgcggtcgct gcctccgcga
    22801 gcccttccat accccatgtt ctcgtgggcg cttctcttct ggtattccat cttcatatcc
    22861 cgcgattcgc gcgagttaaa ttcataaagg aatagccatg ccagtcccgt cccctctaca
    22921 tcggcgcgat gcagggaagg ggaactaccg gcctgtttca cgaagggtgg cgaggtacgc
    22981 gacgaggtcg cggatatctt cgtcccggag gagtgtcttt ggatacatgg ctgtgccagg
    23041 gtggccgcgc ttgattgagc ggtagcggtc ctcgtcgttc tgcgatttca gcgtggcgga
    23101 atcccggagg ttcgcgggcg gcgccggaag cgcgtcaagc cgtttgatca ggtcggcaga
    23161 catcggcggt cttcgattca aatgtccttc gattccatgg cagatcgcgc atccaccgag
    23221 ttcatgatag agctgccgtc cgcttttggc atcaccccgc agcgaggtat cgggttcggg
    23281 taggccggcg ggtggcggca ccgtgccgcg aattgaggcc aggtagggga gcagcgcaag
    23341 aatctcttcg tcggagaggg cttccttcga gagcgggtac atggcggtgc ggagatgccc
    23401 gtgccggatg acctcgaacc gttgtttgtc ggtggtgagg gtgagcgtgg ccgcctgtcg
    23461 caggtcgggt ggcgcaggcg agagatgttc gacattgtcg cggatgttct tgctcagttg
    23521 cgggggcaac cggtctctgt agccgtcgat gccatggcag gtggagcaga gacctttgcc
    23581 gttgaagatg gcccggcctt gctcagcatc cgccggtccg ttgcccggct tggtctgtga
    23641 cggcgatcct gcccagagaa gtgaagcggc aatcaatgag gtcgcgaggc agacgatccc
    23701 gatgagggca attccggggc ggccccttcg agtcgggcgc tgatgggagg atgtcggcat
    23761 ggtcagtgtc ccggtagcct gcctgtcgga cctgtttcac tccatcataa tcgaggaagg
    23821 aggtgcagtg ccatgcctga ttatggagca gagtcatggt gccccctgcg ggggtctggt
    23881 cacgcgcttc aggatgccgg tacaatgcgc agcagtgagg gacacatgat gtacacggcg
    23941 atgaatatga cagaccaccg tcaatgcgta gccctgtctg gctgttttcg cggcaagctg
    24001 tgcgaccagc tcatgcacag acccggccgt tctgtgggca agggggaact cgtctacggc
    24061 ctcggcgatt ccgcgcagag cgtcttcttt cttcggcggg gattcgtcaa gttgacctct
    24121 ctgaccgagg atggccgtga attgattttg cgcctgcatc aagccgggga agtgttcggc
    24181 gagctctgcc attgtaccgg ggaacgccgc gaacaggccg tggcgatgga agacagcgag
    24241 atcgtcgaac tgaacttcga tgaatttgtg gctcatctgc agaacaatcg tccagccttc
    24301 ctgctgtttc tctccaacgt cgcgcagcag ctgtcggcgg cctacgatca gattcagacc
    24361 ctctcgttca gcagcaccat ggagcgcctg gtgcggacgc tgggccggtt ggccgatgaa
    24421 ttcggcgagc cggacggcga atgggtgcgg ctcacgcatt atttccggca ggacgatctg
    24481 gcgcagatga tcggcgcgcg ccgggaagtg gtctcgaccc tcttgaacca gttgcgcgac
    24541 cggggcctga tcaattacgc gcggaaagac gggctgctgc tgcatcgtgc cgggctcgag
    24601 ggatttctgg tttccgccga aaagtctcct gccaatctca gcgattccgg cttctaagac
    24661 cacgtttctc cccgcaatgt tatcccgcta acttacagaa ctgctcccgt tctctatcct
    24721 tgccgctcga tgctaccgcc ggtgtcggtt gatccggcgc gccattacta ttttctaaag
    24781 gagagttgcc atgcagaagc tggttcgagt gttgcctgtg ttgatgttga ctctatcccc
    24841 gattgcctgt gcccattcgg ctgattcgcc tgcaataacg gtgtccccgt cggcgacggc
    24901 gcacccggcc aaggtggcgg agaccaaggc cgtgcttcgg gatctctggc tgggccatat
    24961 cctctcgatt cgcaatgtcg ctgtcgcgac gatggataag aatgcgtctg cgcggagcgc
    25021 ggccgaagcg ggtgtggttg ccaatgccga gcagattgcg cggtccatcg agccgtttta
    25081 cggcaaggcc gcgtccgata aactctttac cttgttggcc ggtcactacg gcgccattcg
    25141 cgatcatctg gatgcgacag tggcaggggc ggcgagccgg caggaggcgg ccgtgaaggc
    25201 cctcaccgcg aatgcggcag agatttcgac gtttctcagc ggcgccaacc cgaatttgcc
    25261 gaaggatacg ttgatgggac tgctcatggc ccatgccgcg catcacctga cgcagtttca
    25321 gcagttgaaa gacggagact acatacatga ggcggagacc tggacgggta tgaagcaaca
    25381 catctacgtg gtgtccgatg cgctggcgca ggcgttagcg gcgcaattcc ctgcgaagtt
    25441 ctaggaggac tattcgcgca ttgactgtgt ctcgtttctg attattttcg gaggccggat
    25501 tcaggcagcc aggagcggag accccgccac ctggctgccc tcctcgacgt gacttcctca
    25561 cgccggtcga tctcgttcgg ccctctccct tccggccctc cgcttctgct cgctcagggc
    25621 gggagatcgt tccggtacaa ctgctcaata ctacgtacta gtggttttct ctgcgcctcc
    25681 tatttgagcg gtcattccgt ctcagtttgc gacaacaatt ggaaccattc tgtttcgccg
    25741 gaaccgcacg ctttttcttg aaaacgcaac ggccacttgc ctagagtgca tcgcttgaag
    25801 atggtttcat tatcatagga ggacggtatg cgcagaaatc gtgagcaatt tgtgttcggt
    25861 ggggccatgg tgctgatcct ggtgatgagt ttggcccccg ccttctctca ggctgccgag
    25921 tgggtgcatg aggggatggc cgaagagggg aaagtcacga tggggtttcg agtgggaccg
    25981 agctttacca cgcaaagtgc cggtgtgtcg acggtcggtc cggcgctgaa ctttcaaggc
    26041 atgtacggac tgaacaaatg gttccgtctc gggatgatgc tggagtggga aagtcacggg
    26101 atcgatgggc ctcgaagcgg ctccgtcgat accattacgc tgcttccggt gaacctggag
    26161 tatcgcccgg gacatttcgg taatttcatc ccgtacttca ccaccggtct gggggtgaac
    26221 atcaatacga aggatgtgaa agatacgttc gcctggcgga tcggcggcgg tatcgactat
    26281 gccttgacca atctggttca gggcgcgccg aaaggcctga tgttgaatat ggaagcggcc
    26341 tggaagcgta accatcccga cagcgacggg tccaccatgg gcctgctctt cggtgtgcgc
    26401 cataccttct aatcccgact tcaaacgttc caacgagtcc ctgaacggga atgacaccaa
    26461 acgcgggcag tcagtagcgg ttacgccgct gctggctgct ccgtcagccc cttattcgag
    26521 cgatcacctc tgccaggtct gcaggactcc ggtgaccctg ctcactctct gttgaaattg
    26581 ctgctgatca tcttctgagg gggggcaaga agagctcagg aggagtaggc cctgttctgt
    26641 gtgagcgggc gggtcgagcg gtgtgccatg gctgaaatct cctcgcgatc agccgccgga
    26701 gggcgtatgc cgaagatatt cgcggagcag gtcccgccag gtgatgatgc cttcgagatg
    26761 tccggttgtt gtgacgacgg ggaggcagga cacgcgatgc tcaagcagca gtcgtgccgc
    26821 ggcttccacg gtcgtgtcgg ctgcgaccgt gatgagtttc cggctcatga tttgatgggc
    26881 gcgtttgttg agcgtcgccc ggtcgcgatc ggtttcggac agtgtcccga tattgggact
    26941 caccgccttg agcaggtcgc gatctgaaat gatgccgagt agcgtagggc cctggacgac
    27001 cagcaggtgg tgaaaatgat gttcattgaa caggtcccgc gctcgagcga gggaatcgtc
    27061 catggtgacg gtgacggctc gtgtcgacat gatggcacgt actgtcggct gtgcgatggt
    27121 gtcggagggg acgaccgggg gaggcatttt cgcggaatca ttaagacctg attgggacat
    27181 ggacggtcct catgggcagg aattggaacg cgcgtgtcgt tctgccttcc cgctctgctt
    27241 tccttatcgg cgggcgggag accattcttg agagtttggc gcgacttgcg cgcggggctg
    27301 gaaggccgga tcgaatggcc gattgcgaat ggatgttgtg cgggacggtc tcgcccgggc
    27361 gtccagtttc tcgttcggct gaggtgtttc tgctcgtcat gtgtatccgc agggcgggag
    27421 ttcctgatgg ttcggcttga tggcgaagtc ggtctgtcgt cggcgcggga gccgaccgca
    27481 gactccctgc tgtttcattc ggccccgatg tgttataagc tgtctgtttc tctcgccgat
    27541 caatcatggt tcagtgcagt ggttcgtaga atcaatccag accttcgggg atcgcacgat
    27601 cgtcagaccg tcagttccgg gggcttcaca gggggaatga attaggatga gcaagaagga
    27661 aatcgatgca gcgcggcgac tgatgagcct gatgttcaag gcgcatccgt ggcacggagt
    27721 gtccatgggc gaccatgcgc ctgacttggt gacggcctat atcgaaattg tgccgaccga
    27781 tacggtcaaa tatgaggtgg acaaggccag cgggtttctc aaggtcgacc gtccgcagcg
    27841 attttccaac tactgcccgt cctattacgg gctgattccg caaacctatt gcggcgagcg
    27901 ggtagcggaa ctgttcggcg cgcgggcggg ccggccggac atgatcggag acggcgatcc
    27961 gctcgatatc tgcgtgttga ccgagaagac gattccgcac agcgatattc tgttgacggc
    28021 ccggcccatc ggcggattca gcatggcaga cggcggcgag gcagacgaca agatcatcgc
    28081 ggtcatgcgg gacgacgccg tctacgggtc gtttaccgat ctcaaagaat gtccggcggc
    28141 actcctggat cggctccacc attattttct gacctacaag cacgcgccgg gatcgacgca
    28201 gcacaaggtc gagatcacga gcatctacgg ccgcgatgag gccatgaaag tcattcacgc
    28261 cagtcacgac gactacaaac agaaattccc cgagttagaa ttcatgtggc ccgccagcat
    28321 gaacagctag tactaccaag aaaggtttat ccccccctga tgaaacacac cacgacgcat
    28381 tcgcagacag agccgacggc caagggattc tctcccggtt ttgccgcgct cggattagaa
    28441 gcctcgctgt tgaccaccct ggaagccttg ggctacgaag agcccacccc gattcagcgc
    28501 gaagccatcc ctccgcttct ggagggacgc gacctgctgg ggcaggccgc cacgggaacc
    28561 ggaaagacag cggcgtttgc gttgccgctc ctgcagcgga tcgcccatgg gccacggcag
    28621 cggcccacgg cattggtgct ggtaccgacc agggaacttg ccgtgcaggt gagtgaggcg
    28681 gtgcagcggt acgggaagga gctgcggatc ggggtgctcg ccctgtacgg cggtcaggcg
    28741 atgggaccgc aactgcaggc gctccggcgc ggggttgaag tcatcgtggc gacgccgggc
    28801 cgggccttgg atcacctccg ccgcaagacc ttgaagctgg ccgatctcca agtggtggtg
    28861 ctcgatgagg cggacgaaat gctcgacatg ggcttcgccg acgacctcga tgccattctc
    28921 gaacaaactc cggccagcaa gcagaccgcg ttgttctcgg cgacaatgcc gccgcggatt
    28981 gcgtccatcg cccgccggca tttgaagaat ccggtggatg tgacgatcgc ccgtgagccg
    29041 gtgaaggcag gcgccgcgcc gcgcgtgcag cagaccgcct atgtggtggc gcgacagcat
    29101 aaggtgtccg cgctggcccg cgtgttggat attgcgacgc ccaaatcggc actcgtgttt
    29161 tgccggacgc ggttggaagt ggatgaagtg acggcggcgc tgaacggtcg gggttatcgt
    29221 gccgaggcca ttcatggcgg catgagccag gtgcagcggg atcgcgtgat gcaggcgttt
    29281 cgctcaggac aaaccgaact cctcgtggcg accgacgtgg ccgcgcgcgg gttggatatt
    29341 ccatccgtct cccacgtgat taattacgac ctgccctcgt cgttggaagt ctatgtccat
    29401 cggatcgggc gaacgggccg ggcggggcgc gaaggcgcgg cgatgactat catcgagccg
    29461 cgtgaacagc ggttgttgcg cgcggtcgaa cagcatacca aagcgaaaat tacggtcgct
    29521 gctgtgccat ccctgggtga tctgttggcc aaacggctgg agcggaccaa ggcctccatt
    29581 caagaaacct tggaggccgg cgagctcgaa gattttcgtc gtgtcgtgga agcgctgtcg
    29641 gcgtcggcag agccggcgga tatcgcggcg gcggccatca agctggtcta ccggtcccat
    29701 ggcggtgaac gggcggaaga agaaatcccg gcagtctcga ccagggcgcc ggagccctat
    29761 cggccgtcgc gttcttcgta cggttcggct cctcagcccg gggatcgcgc gcgtgcgccc
    29821 cgcggtggtc cgagtcgcgg tggtcgggca gccggaaccg tgcgggtcta tgtcggggcg
    29881 gggcgggcgg caggtatcag gcctggcgat ctcgtcggtg ccatcgccaa tgaagcgggc
    29941 gtgccgtcgc gtatgatcgg ggcgatcgaa gtcgaggaac gattttccct ggtggatgtg
    30001 caggaagagg cggcccggca gattatcgag gccctggggc ggacgcgcat caaggggcaa
    30061 aaagtcgccg tgagattgtt cagagattga gcaacgaggc gggccggccg ttgacggacg
    30121 tcacggccgg ctcaccgcgt gatcctccgg agtgtgtctt ccgtcaaccg ttcccaccgt
    30181 cgagtccggc cggtctatcc ccccgcgcag gctgagccat caccaccgtt tcggcccggc
    30241 atcttccttc ttggcccttg ttgcctgacc ggttttgcga taccatcccg cacagacatg
    30301 atgcgtctca cccataccgt gccggccgcc tcgcgatcgt gagggtgtgc cggcagagtg
    30361 tgcgtcacac ctcattatca gatcagttcc aggcagacag tctctgtggt gcctcgcacc
    30421 ggcgagccat tacggaacgg gactggcggc gtctgacatt tggtggcgat ccagggggat
    30481 ggcgctgggg tgagactgag ttccaatgag tcacggcgac ggagaggatc tgtgttgaga
    30541 tggcggtgac gctcgcacca tggaaagccg tgctgcggga ctggcaggcg agggcggtcg
    30601 ccgatgtgtt ggcgcagccg cgcgaggact acctcgtcac cgcgacgcct gcggccggga
    30661 aaacacgctt cgcgctccgg atcgcccatc agtatctcgc cgatcgggcc gcaagtcgtg
    30721 tcctggtgat ctgcccgacg aatcatcttc gaacgcagtg ggcttccgcg gcggggcagg
    30781 tcggcattca gctcgatccg gcgctctcga atgagcaggc ctgcgaggcg cgtgactatc
    30841 acggcgcggt tgtgacctat cagcaagtct gtctggcgcc ggaagtgttc cggcgcgcct
    30901 gccgcagtcg caaaactctc gtcatcctcg atgagttgca ccatgccggt gacggtaagg
    30961 attggggcaa agcgctgcgc gaatccttcg gcgaagcggt ctttcgtctg gcgctgtccg
    31021 gcactccctt ccgctcggat aataacccga ttcccttcgt gcgatatgag cagggcgaaa
    31081 gccaggcgga tttcacctat gggtattcgc attcgattcg tgacggggtc tgtcgcccga
    31141 ttctgttccc gagttacgaa ggcgaactga cctggttgtc ggacgggcgt gagcatcggg
    31201 ccaccttcga agacgggtta accttcgaac gccagcggga acggctcaag accgcgttgc
    31261 ttcaggagac atggctgggc ccggtcttga gcgacgccca tgccgagttg cagcggttgc
    31321 gcaagcagga acaggcggat gcgggcgggc tgatcgtgac catgaatcag gaccatgcgc
    31381 ggcaggtggc ggacctggtt ctgaagctca cgggcacacg cgcgctcgta gcggtgtccg
    31441 acgatccatc cgcctcgaag accatcgaaa cgtttgcgca tcacaagcag cagcagtggc
    31501 tggtggcggt gaatatggtg agcgaaggcg tcgatattcc tcgcctgcgg gtcggtgtgt
    31561 atgcgaccaa cgtgttgacc gagatgtatt tccgccaggt cgtcgggcgg tttgtgcgga
    31621 tgcaggataa ggtgccgaaa ccgcaacggg cctggctcta cctgccgaag gatgccacgc
    31681 tggtgcatta tgccaagcgc atcaaggtcg agcgcgatca cgtcctcgaa gagatcatgc
    31741 cggcggtgca acggacactc ttcggcacgg cggccacctc gctcaaggag tacattcccc
    31801 tcaacggcgt ggccaggctc gatgccttga tcggtgcgga cgagggtgat tcggcgggtg
    31861 acggcaaggg gcagccggaa ccggcggtcg cgttgcatga ccagaaaaac accttgcggg
    31921 acaaacatcg ctctctggtc ggggcggtgg cgcggaagtg cgggatggat catcggcagc
    31981 tgaatgcgga gttgatcaag cggacaggcg gccgggtcga tcaggcgacc atcccgcaac
    32041 tggagcgacg gatcgccctg ctggaaaagt ggcgtgattc cggcttcgac aagaagcgct
    32101 gagcggctga agtgtggtaa tgatggcagg ctccatcggc ggatgtgcga tttcgttgaa
    32161 tattgatgtg agaggaaggc aaccatggct gggaaacaag tggcgcgccg gacgagtgcg
    32221 gtcgccaaga agatcacgaa gcaggcggtg aaaaaatcag gaacgaaaaa agcggcgcct
    32281 gcgcgccgcg caaagacgcc tcgcgccaag cggccgtccg tggcggtggt ggtgctgtcc
    32341 ggttccactc cgcttcgccg gaacaaggcg gcggagttgg tggcggaaga actgcaattg
    32401 gatctggcaa aggtgaacct ggcttcggtg gtcagccgtc aggtcggtga gacggagaag
    32461 aacatcaaca gggtgttcga tgaggcggaa cggagcggat cgattctctt cttcgacggc
    32521 gcggatgcgc tgttcggcac gggccgggcg ggcaaggcgg gcaccagtca ctcgaccgct
    32581 gccggggcgg cccatctgct acaacgcatc gaggatcata acggcctggt cattctcacg
    32641 accaacggga aaagccggat cgatcaagcc ttgtcgcggc aagcgaaggt gtccgtgatg
    32701 gtgggcatca agaagaagcc aaagaagaag acggcctgac gagtcgttct ctcaatggtt
    32761 gttcgagaag cgatgtcacg agcacaggat gctcaaaaag gctgctcagc aaggccgcag
    32821 cgagcgaggg gccgaggcgt accctcgggg tacgttgagg gtccgagcga tgcgagaacg
    32881 cggctgacag actttttcag catcctgtga ggattattcc gtttcttcgc tggcctccag
    32941 acacttcgtg cactgttcca gatcgggcac gccatgggcg ctgcagaagt gccgatggca
    33001 gaggccgcag agcataaagg tcatcttgat gcggtcttcc gcttcgcagt agtaacagtt
    33061 ttttgcggtt cttgccttcg gcatggtcct tctcctcgaa acatgtgtcg gttcaattac
    33121 cagtcattga gtgagtgcac gagtcgcatt ccgtcggcgt cagtgcgctt cgccgttcca
    33181 tgccgatgat cgggcatgag ggcggcagga cctgaaacca tgacaggggg cgggcaaggc
    33241 acctaggccg agttagcctg aggcgctaag ggcagctaga ggtgtgatcg tgctcgcgtt
    33301 caccattcca cagggtcggc cggcactccc gtggactgat gctccttata acacattttg
    33361 ccgatcgtcg gcttggccct gaccgggcgg ccgccggcgg cgttctggac atctgtgaat
    33421 cgtgaagcgt atctcgtcag ggacgcccac tctcgatttc cggccatgtg cgatgaactc
    33481 tatgccatac gctcttttct gcggacgaga gacgaacgac gtttcacgaa cgacggacta
    33541 ggaggacggc ctttttgcgc atcctgctat tgtgcctgct cgacttcttc cctcaacaga
    33601 aacttgatcg acgggttcga tttgatgcaa taggccagca ggtgttcggt ggtgaacaac
    33661 atgacgccgc ggccttgctg gtcgacgagc cggatggtgt cggacggcca gtagacggcc
    33721 gaaatcgtgg cgggatcacc gacgatccag cgggccagcg catggggcag gcgctccacg
    33781 gatgtcttta ccccatactc attctccagg cgagacatca cgacgtcgaa ctgcaattca
    33841 cccaccgcgg ccagcaccgg ttcgcggcgc acgtggtccg gtgaataaaa gatctgcatg
    33901 accccttctt cttccagctg cttgagcccc ttctgaaact gcttgtgctt cgaaatatcc
    33961 tgtgtgcgca acaggccgaa acattccggc gcaaagtgcg gaatcggatc gaaggtcagc
    34021 gcgggatcgg aggtcagggt atcgccgatc gtgaacaggc cggggtttac caacccgacg
    34081 acatcgccgg ggtaggcttc gtcgatcgtt tcgcggtcgc ggccgaacag gcgatggggg
    34141 cgtgtcatcc ggatcttgcg gcccagcctg gcgtgatgga ccatcatgtc tttttcgaag
    34201 cggccggagc agatgcggag gaaggccatg cggtcgcgat gctgcgggtc catgttcgcc
    34261 tgaatcttga acacgaagcc ggagaacgct tcgtcggtcg gttgtatcaa tccctgggcc
    34321 gtctggcgcg gtccgggagg cggcgcgagt ttggtaaagg catccaggaa cggcccgacg
    34381 ccgaagttgg tcaacgcgct gccgaagaag accggcgtca gttcacccgc gaggaaccgg
    34441 tcgcgatcga aggatgtgcc tgcccccgtc aacagtccga tctcttcctg caggggcccg
    34501 taggcatcgc cgagggtttc cgccagtttc ggattcggaa aggcctcgac cgtcatcggc
    34561 gcgcgccgtt gattgtgtgc cgtacgctgg aagaacagca cctggcggtg ttggaaatcg
    34621 tacaggcctt gaaagcctga cccttcgccg atcggccaat tgaagggcac cgctgtcatg
    34681 cccaaggtcc gttcgatttc atccagcagg tcgaacggat ggcggcccgg ctggtccatc
    34741 ttgttgatga aggtcaggat cgggatgcca cgcttgcggc agacggcgaa gagtttcttg
    34801 gtctgcggct cgataccttt ggcgccgtcc agcaccatga cggcgctgtc cacggccatg
    34861 agtgtgcggt acgtgtcttc gctgaagtcc tggtggccag gcgtgtcgag caggttgacc
    34921 cgcgcgccct gatagtcgaa ctgcagcatg gtggaggtaa tcgaaatgcc gcgcgcctgc
    34981 tccaattcca tccagtcgga cttggcttgg cgctggctgg agcgcgcctg caccgcgccg
    35041 gccaggtgca ccgcgccggc atagagcagc agcttttcgg tcaaggtggt ttttccggcg
    35101 tccggatggg aaatgatcgc aaacgtgcgc cgtcgcagca cttcgcgccg caattcatcg
    35161 gccgcagatt tctcaaccgg cagggacata ggtcttctct ttgtggttag gggaagccaa
    35221 cgggacaggg gatcagggca tcgcctggcg tcgctgggtg ataactcttt cttttatcag
    35281 tcggagtggt gcggcggcaa ggagggggag aggggcgtgc agtcgggcgc tccccgagaa
    35341 gacactgccg gctcaccgcc tcgccggcgt gcgcaaacct gacgctcgtt attcctcgcg
    35401 tcgcgcacct cgcgcaacgc gcggcctcag aaggccctcg ttggacgcgc gcactcagga
    35461 gcacccgact gcacgccctg ggtgaggggt aggaggggat tcagttggac ggcgcgcagt
    35521 gggagatcaa ccagccccca tccctaggga gaattggaaa ttgagaaaat ggggaatgcg
    35581 cgcgatcgga atatgctcgt cccatgcgcg cagtgagagc cgccccgacc cctccccttg
    35641 gattggtgga gcgttggatg cgcacaggtt gggaccttcc tggctgcccg ccctgggtga
    35701 gggatagagg gggattccgg atgatgcgcg cgatcggaat gtgcccgtcc gatgcgcgca
    35761 gtgacggctc cctgcctttt cccttttagc cgaagttgga atagcggaca cgcggactgg
    35821 gagatcaacc agccccatcc ccgggatgag ggataagggg tgatttcgga cgacgcgtac
    35881 gttacagctg tacgtgtaag acgcacacag tccggaccac cgagggtgcg ctaccaggag
    35941 aagtggctga gggagagagg cccaatgggg tacgggtcac gttttgcgag acatggggat
    36001 ctcgacgagg gagcgcaagg tctgacccgc aagcgcctgt tcgatggcgg tatcgcggcg
    36061 gcgtaacacc tcacggacag tgtcctcggt acccagcgag accgtcgcat cggcggcacg
    36121 tccttggtgg gtggcatcca ggacctcttt gatggaaatc aactcaggcg gcttggtgag
    36181 actgatacct tcgggattgg ccatgaggcc gagcagtccg gtttccacca gatgccgtac
    36241 ctgttccgcc acgagcgaga cgggaaggtg caactcgatg gccaggtcgc ctgcaggcat
    36301 cggtccctcg cccttcagat attgtcgagt caggaccacc agcaggttca gggccaaccg
    36361 ctctcggaat gcatgggtgc cttgctgcca gagcaaacgc gacacatagg cggtgggata
    36421 ttgatggaaa aacgagaatt gcgcgccgat gagcacgatc agccatccca catagagcca
    36481 cagcaaaaat agaaccagca ccgcaaagcc ggaatagatg gcgctgtatt gggccgactg
    36541 agccacgaac atcgcgaatg cttctcctgc aatgccccat aggacggccg cggtgatccc
    36601 gccgaccagc gcggagtcga atcgcacctg tgtgtggggg atgaacttat aaagaaaggt
    36661 gaagagtccg ctcaaaatga cgaagggcag cagtcggccg gccaggagca tcaggtaccc
    36721 gaatggttgg atttccaaca cccgctgcac cagggtatta ctgtgaacgg aggcgagcag
    36781 cccgaacgcg gtcacgacca gcaatggacc gactaacacg gcgctcaaat aatcggcaaa
    36841 cttccgtccc cacgatcgtc cttcccgcac cagccaaatg gcattgaacg cttcctcgat
    36901 tttgtcgatg agcgaatagg tggtgtagaa aagccccgca acgccgacga tgcccaacac
    36961 gccgatcttg aggttgtcca cgaagccgat gatcttgccc gtgatttcct gcccattcgg
    37021 gcccagcggt tccaatgcct gggccaggac cggttcaatc tgctggtgga ctccgaaggc
    37081 ttttaatacc gaaaacatga cggcgagaaa ggggacgagg gagaggagtg tggtgtacac
    37141 caatccggcg gcccgggcat caagcagtcg atggcgaaac tccagtgcca cggcgatcac
    37201 caaccgcatg gcatgaatgc cgatgcgctg gagggtgggc acggaggcgg gatcgatcgt
    37261 ccacaggtct gccttgaaga actgtgggat gcgtgccagt ttcgtcatgg tccatcaggc
    37321 ctctgagaga aggtcactgt gccgcgaacc ctcggttcgg tacagcctgc gatcggcagt
    37381 cccttgtatc tcttttgata ccgcgcctga tggtttggat acaagaccgg tttgcccgtg
    37441 agtgttgata gaaatgctgc catttgcatc acagtaaaaa ataaggagag agtgatcctt
    37501 cgatagtgtt ggcacatgca ttgctcggat agaagaatgt accgttgcgc gcaaaaaata
    37561 tcaggagggc atgccgatga cgaccactca agaaccgccg gaggatcgtt tctgcgacct
    37621 ggtgatgaag ggcggcatta ccagcggtgt ggtgtacccg aaggcgatcg aactcctgtc
    37681 tcaccagtac cgcttcaaga gcatcggagg gacctcggcc ggcgccatcg cggcggtggt
    37741 tacagccgct gcggaatatc agcggcggtg gaagggctct cgggccggct tcgacctgct
    37801 tggaacgctc ccgacggagc ttcagcgtcc ggtggcgccg ggcacaagca agttgctcag
    37861 cctcttccag cctcagcctg ccatgcgacg gctcttctcg gtgcttatcg gagccttgaa
    37921 cagcgagaaa acctcatcac gtatcgctca gatcatcacc ggcttcctac gggcctattg
    37981 gcccgcaacg ctggcagccc tggtgatcgc ccttgccgtc ggggctgtag gattgggttg
    38041 gttcgcggca tgcctgaccc tcgttatggc gttgccactg ttggtcggcc tctgggtcta
    38101 tgatgacgtc acgcgacgaa tggtcgctca tgatcttggg ctgtgcaccg gtctgacgga
    38161 agacggaggg aacgaagcgc tcacaccctg gctgcacgcg ctgatccagc aggtggcggg
    38221 acgtagtctc agtgaccctc cactcacttt cggcgacctc tggaacgcgc ccggctttcc
    38281 tccccgctgg ttgaacgtac cggccgaccc caaaccacgc tcgatcgatc tgcagatgtt
    38341 ttccacgaat ctgtcccatg ggaggcctta catctttcca ttgcccgaga ggaatcccca
    38401 gccgaccagg ttccatgacc gcgaccgtct gtacttcacg gaaagggaaa tgaagcggtg
    38461 cctgcctgtc gacgtggtgg cctggatggt agagaagtcc aagccctaca ccgttgagcc
    38521 gggcagagaa gaaaaagatc ctcccgccga tacagaaaag gggatgcggg aggtgcctga
    38581 gccccgcgac tttccggtcc tgttggcggc acgcatgagt ctgtcatttc ccttcctctt
    38641 cacagctgtt ccactccatg ccatcgatca tgacccgctc agagcgcgag agtttcgacg
    38701 ctgttggttt tccgacggcg gtatcagttc caactttccc atgcacctct tcgacgggct
    38761 ggtgccgatg tggccgacct tcggtctcaa cctcgagccg aagatcaagg gcagggagat
    38821 ggtctttctt ccggaacagt atgaccaagg ctatggggaa cgttggaatc acatacctga
    38881 aaaaaagggg gccggcacat tgggcgggtt catcagcgcc atcgtcggca caatgcagaa
    38941 ctggaacgac aactccctcg cacgcatgcc gggagtgcgg gaccgggtgg ccagggtcag
    39001 gttgagcaaa gaagaaggcg gcatgaatct caatatggag gcgcggctca tcaagaacat
    39061 tgccgagcgc ggtgtcgatg ccacccggga acttctggag cgattctccg gaccctcacc
    39121 cgatggcggt gcagcgaagg gatgggatga acatcgattc gtccggctgc acgtgttgat
    39181 aaaaatgctc gcagccagat cgccgggagt cgtcggcgcg ctcacgtcat gcggacatgc
    39241 caccgacttc tcgacgctgc tgaagaatat gatgaacgcc aaggacaagc gtgggaaccc
    39301 cctgccaccg ccgggatatg aggaaccgat gaccaaggaa caatgtcagg agctggaagc
    39361 gttcgtcact gtgctgcggg atcttgcgac cgccatgacg accccggcca ggccgatccc
    39421 gttcaagccc atcccgaatc cggagctgcg ggttcgcccg ccactataag gtgaacgcga
    39481 tggcaggagg gtgaagagcc tcgccttgtt gggagcgggg ctcttccttc atgcacgtcg
    39541 tgcgatcaca cgagaagatg gctacttgcc caacggattg gtcagcgcgc ctttcgcgcc
    39601 ttccttgacc cctttgacgt cttctttgac ctgacccacg ccctgcttgg tcttatcgat
    39661 gtccagcttc ttggtgtctt ctttcgtttg ggtcgcatcg gtcttgagtt tgccggtggc
    39721 ttccgtcagc gacttgcccg tgcccttcat cgcctctttg ccttcggcta ccagaccctc
    39781 ggcatgggct gatggcagaa acgacgaaac cgagcttgca ccgagaccgg cggcgactgc
    39841 gatagcgacg atcaatgact tctgcatgac accctccttg gtgagcggac accactcact
    39901 gcatccagga taatgcactt gcgcacacat ggtcggcctg ctcaggccaa tgccctgttc
    39961 ccgtacggtc actataacag gatagcccaa gttttgtttc gcgaccttat ttaagcaggt
    40021 aaagcatgga cctgccgtct ccgggaaagt agaatgccac cgttcattca tccggcgatg
    40081 gatcaagggg cacttggaaa tggaaaacgc gatcaaatct cttggcagaa acatgttcca
    40141 tcagcccatg ccgcatctgt agggttcagc aagaccgcta tcaccaatgc aactacccga
    40201 tgggctctac gacaagctgg ttactgaatc ggtggcgcag ttaattgctg gccttccaga
    40261 ctcgtttggg cacacatgtt caatactacc tttggaagaa gtatcagagc gaatcgcgga
    40321 cgctctcgcc agacaagtga cacgcttgct agatgaactc ggcgggcagg gggcagagaa
    40381 ggcaaaacga cagctcgatc tcgtgaacgc actgctggtt caccttaggc agccggccgc
    40441 aggaggtgtg gacccgctgg tcgagccacc tcagatactc cgagccattc atagcatcgg
    40501 agctgcaccc gaagctccag cgactggact cgctatccct tggctgttta ctgcgggcaa
    40561 aggttcccca tcccttctga cagaacttcg ccgtgagatc gcctgctgcg accaagtgga
    40621 catcttggtg agcttcatca cagtggcggg ggttcgaaag ttgttcgaca ttctccagac
    40681 ggccacagcc gtgagcgctg tgggccagcc acgaacgcga cttcgaatcc tcacgaccac
    40741 atacactggc gcgactgaga tggaagcgct ggattatttc ggacgcttgc caggctgcga
    40801 aatcaaggtt tcgctcgatg gaagacggac acgtctccac gcaaaggcat ggatatttca
    40861 tcgcaaaacg aggtttggct cggcctatgt cggcagtgcc aatctatcag gctcggcgct
    40921 gctcggagga ctggagtgga cggtcaaatt cacggagcaa gggcaggctg gactatttgc
    40981 tcgcgcgaag gcgcacttcg agacactttg gaacgacggt gaattccagc aatacgatgc
    41041 ggcaaaccag gagcatcgct ctgaactctc gcgcgccctt aagcgggagg cgggtgacca
    41101 gatttgtgcc tcaactacat tcttcgacct tcagccgaag gactatcaga aggacatgct
    41161 ggagcaactc acgctggaga gggagcatgg taggtttcgc aacctcattg ttgccgcgac
    41221 gggcacaggc aagacagtag ttgcggcgtt tgactaccgt gcagcatgcc aagctatggg
    41281 cggacgtcct cggttgttat tcgtcgccca tcgtaaagag attctgatgc aagctcggcg
    41341 aacgtatcgc gaggtgctgc gtgaccactc ctttggtgag atgttggggg gtggttctga
    41401 gccgcagagc tacgaccaca tattcgccac catcgacagt gtgtgcgcac gcgacctggt
    41461 cgcaagatgc ggccccgact attggcacac ggtcgtggtt gatgaatgtc accggttggc
    41521 ggcggaccgc ttcgatatgt tcgtgaaagc agtttttccc aaggtgatgc tcggcctaac
    41581 tgccacaccg gaacgttcag atgggaaacc gatctttccc tacttcgaca atcgccccga
    41641 tggttcgcct gccgttgagt tacgcctctg gcatgcgctg gacctccaac tactcagccc
    41701 gttcgagtat tacggctgcg acgatgacac cgacttttcg gaagtgcctt gggacaaacc
    41761 tggtgagcga gaagtcattg ataacctcgt gactgggaat gacacccgtg cgagactcgt
    41821 cgttaacgaa tggcggcgac tgactggcaa ccccagacgg agcaaggccc ttgtcttctg
    41881 cgtgagcgtg gcacatgccg aattcatggc gcggaagttt aacgaggccg ggctgccttc
    41941 gctctgcatc gttggctcga ccgatgcaga tgcccgccgc caagggccag aacgtttagt
    42001 gcgtggagaa ctctgtgcac tcgtgacgtg cgacctctac aacgagggcg tagacctgcc
    42061 ttcggttgac acactgttgt tgcttcgccc cacacaaagc cccgtgctct ttcagcagca
    42121 gattggacgt ggattgcgtc ttcatgaggg caagcaaagc tgtcttatct tggactttgt
    42181 cggccgatac cgcgaagagt ttcggttcga ccgtttgctt tcggcgatta ccggactatc
    42241 gcgcggccag ttgagtgaac cagtggagaa gggatttagt tcgctttcac ctggctgcct
    42301 cattcagctg caacaattca ctaaagagca aatccttcgg aacctgcgga ccgccatgaa
    42361 ccaatcgtgg cgacgacttc gcggcgagct tcagagttat gctgccctgc gtggacggac
    42421 ggctgtcaat ctcggggatt tcctgcgaga gcaggcagtg cagctccatg aaatttatcg
    42481 cagccatggc cgtagcggct ggactgcctt gcgacgcgac gctgggctag taaccacacc
    42541 tgaaagccca gcggacgaat attttggccg ccgtttctca gacttgttgc actgtaatga
    42601 ccccgaacaa acggaccttg tcgttcgcat cagcgaaccc gccgtgcaat atcacaccct
    42661 caacagccgc gagcgttgcc gacttcaaat gctcgcatat caggttgacg gccagcatca
    42721 ccaggcaggt actggcgaag ccttcctcgc cagactctca cgggcgccag aaatctacgc
    42781 tgaacttggc gaactgggtg gggttcttcg ggcgaaaggc aacctgcgct ttcgcccggt
    42841 accggggcta atcgacgtgc cgttgtgcct tcacgcgagc tacggcattc gtgaaatact
    42901 aaccgcagtc ggttggctga ccgcagaaaa aagaattccc ttccaagctg gtgtacttcc
    42961 actgcaaaac cggaaggtgg aactgctgtt tgttactctc gacaagtcag agggtttcca
    43021 cgatcgaatt gcctaccgtg attacgccat cagcagtgaa cgatttcact ggcaaagcca
    43081 gaattcagcc gggcctgata cgccagccgg tcggcgttat cttgagagcc ccagaaatgg
    43141 ctggtcgttc caactcttcg tccgtcgaag caaaggggat tcctatcggg cttgtggtcc
    43201 ggtgacgatt gagaaggcag aaggagaaaa accgatgtcc atagaatgga aactcggggt
    43261 acttcttccg gtccatctgt ttcaagaatt cagcatcctt aggggacagt aacatgcatt
    43321 gtgattagct ggagagggcg ggagtgtctt ctgctaaatt accttcctcc aggacgcaga
    43381 ttaggcgtaa cccattcatt ttgactgatg aatgcttttc tagagcaggt ggctggtttt
    43441 ctgctgaggt cttttgtgaa attcttcagg aatgtagcgc tttaaaatga taaataaggc
    43501 gaaaatgtac gctttcacgc tgggggaaca tatgccaact aatgacgaaa gggcagaacg
    43561 tgccagggaa gttctcgaaa tgtacgcggt acaatttggt gatccttacg attcacgcgg
    43621 gactttgatc gacgtgttga cagatctcat gcatctggca gcccgagagc ctgaactagg
    43681 cttagaattt gacagtagtt tgaaaatggc tcaatttcac ttcgaggctg aggccgaaga
    43741 atgtctggat acttaacgag atgaaggcag aggcggaaaa tctagacacg cagaagcgct
    43801 atcgaatttt catcttctaa gactgtcggc catacgccca tgaccagccc ccatcctcgc
    43861 gatccactgc atggcataac gttggagacc attgtgacta ccctggtcga gcgccatggg
    43921 tgggctgagc ttggtcgtcg cttgccggtg cgctgttttc tcaataatcc gagcgtcaaa
    43981 tccagtctca catttctccg caagacgccc tgggctcgcg agcgggtgga aggcatgtac
    44041 attgccgaat tgccttgatg gcgctcggcc ggtggcccgt cgatctttcc tcgcttgacg
    44101 tttgactcct cccgcaagcc tgattcatcc gcctcaggga gatcagcgca attgcagcgg
    44161 tcttcccact caaacctgcc gacccgggag atccctcctt ggttcgattc gacaccgctt
    44221 gtacaatcgg gatcagctct atacaaatag agatgaacta tgtctcatca taataagagg
    44281 tgtcggctcg tgcgtatgat gttcgctgtg gcctttgcct gccttctgaa ttgctccccg
    44341 gctccggctt ggctttcgca ggccacagct gcttcatcgg acaaagacac ttcagccggt
    44401 tccaacggac cggctgctca gggtccttcc agtcaatcga catcagactc cctcgtgaac
    44461 gagttgcaaa gcaaacgaga ggctgcggaa aaagagctcg gcgaaatcaa ttcaccgccg
    44521 actctgcgga aaggggcgcc acccgggacc acggacgctc gattgatgga gcgccgctcc
    44581 ttgctgcagc agttggtgca gacgtatgag cagcatctgg atgccttgag gaagttcgat
    44641 ccaatgcgcc tgcgtgtgcg agaggttgaa cgtcagaata aagaatggga cgggttttcc
    44701 accccgccgc cctattcggt gctgatggtc gatgaattgc gcgatgcggc acggtcgatg
    44761 gcgctcacgg cgcagggcat tcaaaccagg ctgaccatga ctgagagtat cgccgacagc
    44821 acgcggcgca atttgaaggc ctcccaagag caggctcgcc aagcctccga acgtctcgaa
    44881 ggcgttcagg acccggtgaa gcgagaggct ctcacctggg atcgtgactt ggctcagtta
    44941 cgcgagcgcg tggagtcggc gcggacgggg atgttagagg ccgaaaaagc gcagatccag
    45001 gaagagctga cggaagcgca gcagcgtatc acgctgctca agcgacaacg tgctgtcgcg
    45061 gcgcaggacg ttgaattcac caagcaggat tacgacaaaa tcaagaaacg gctcgacgcc
    45121 gaccatcagg cgctgatgtc ggagttggac cgggcgctgc tggaacaggc tgcgcaacgg
    45181 caggcagtcg cggccagtga agcgaagctg gccgccgccg atcgcaaggc ccctccttcc
    45241 tccaagacct cttcccgtgg tctcaaggaa gagggggtgt cgcatttcac cgaagccgtc
    45301 gagctgaccc gccttcaatt cgacaatgcc aatttacgcg tggatttcct tcgccaaatg
    45361 gtcaatggcc tggagcgtga acggcagatc tggaatattc gctatgccac cgcccagggg
    45421 gctctgtccg ttgtcgaaga gcgtgatgcg tcgcagaagc tgaccgctgc cgccaagcgg
    45481 attcaggggt ggaaggaata tgggttacag cagctcggca tggtgggaag ccagatcagc
    45541 gatgtggaaa accgactcga agagacgtcc tcgtctgcca ccggtcaata tctcaatgac
    45601 atgctccgcg cctatcgtca ccgggaagac ctttatcggc gcatgcttca acggacggat
    45661 tcgctgttgg ggttgatcga gcatcggcag accgagttcg ttcagcgtga gcaggcccgt
    45721 tccatcgtca cgcgcctgaa agattgggga acatcgtctc tggccgtggt cgggaacatg
    45781 tggaatgtcg agttgttcac cgccgaggat acgatcgagg tggacgggaa gagcgtgacc
    45841 ggacgccgta gcgtcaccgt cggaaaagtg ctgacggcaa tagccctcct ggtcatcggg
    45901 tattggtgcg cggtaatctt ggcacggttc gcagaaaagc aggcgattag ccgactgcag
    45961 gtcgatccga atgtcgccaa catcattcgc cagtgggcct tggcctttct ttttctggtg
    46021 ctcgtgagca ttacgctgac gttcgtcaag attccgatca ccgcctttgc ctttctagga
    46081 ggcgcgctgg cgatcggcgt cggattcggc actcaaaatc tcctgaagaa tgtcatcagc
    46141 gggctgctgc tcctgatgga gcgtccgttg cgtgtcggcg acgtgatcga agtcgatggg
    46201 atcagaggca tggtcaccac catcgggttg cgctcgtcga cgatccggga cgtcaatggt
    46261 gtggaaaccc tcattccgaa cagcaatttg ctggagcgga acctgaccaa ctggacctat
    46321 tccagtttcc gcaaacgtta ttcgatccgg atcgcggtgg cgaccggatc tgatgctcga
    46381 cgcgtgaaag agatgcttcg agacctggcc ggccagcacg gacaaattct caaggaaccc
    46441 gaaccctatg tcctgtttga agacttcagt gagcatgcgc tggtgttcgc cctgcattac
    46501 tggatcgaca tcggtccgag catcgagccg gcgaccgtcg ccagtgatct caggtttatg
    46561 atcgagaaca cgtttgctga agccaagatt gtcaggaaat aacctgccga tcgctcactc
    46621 gacaagctga acctgttctt cacggggaac acacaccaag atggcgccga atgcgacgat
    46681 tttcaaggcc actctccaca tcgccgatgt ggaccgtcac tattacgagg accatacagt
    46741 cacccttgcg cggcatcctt ccgaaaccga cgagcggatg atggtgcgtc tgctggcctt
    46801 tgcgtttcat gcgcatgagg cactgctatt tggccggggg ctgagcaccg aagatgaacc
    46861 ggcgctttgg cagaaggatt tgaccgggct cattgagctg tggatcgagg tgggcctgcc
    46921 ggatgaaaag gctcttcgcc aggcctgcgg ccgcgcccaa cgggtctgcg tctacaccta
    46981 tggcggtcgt gctgccgatc agtggtggaa agagaatctg gctgtgcttg agcggttgaa
    47041 gaatcttacc gtgatgaacc tctcgccgga gggaagccgg gctctggtcg ggcttgcgca
    47101 acgcaacatg gaactgcatt gcaccattca agacggtcag atgttgatcg gtgacgggac
    47161 gaatgccgtg cagattgaat tgacgaccct gaaggaagtg tgaaaggtga aacgtaaaac
    47221 gtgaagcgta tctcgttgcg gacaagagcg tatggcgcat ggcgtggggg agtataagcg
    47281 tatagctgat gacgggagga tgatgtctgg ggccctgcct cctcgctctc agcaatcaac
    47341 cattcgctct tcgcttctga ttcctgctat gagctatacg ctataggctc ttttcttcga
    47401 acgggagacg aacgacaagt tctggagcat gacggcatga aggagaagaa gcgtatcccc
    47461 gtcgacggag agccggttca gtggaaaagt ccgtttgcgg ccttgaagag tcttgaactg
    47521 ccgccgactc caccgcgaga caagacggaa cgccccgcca tggcttcctc aagccaagcg
    47581 aagaaccgtg ggcgggtaga tatcatccgg caaaccgcgc atcgcggggg caagacggtg
    47641 acggtcgtca cgggatttac cggtattggt caggacgaga aagagcaact ggccaaagag
    47701 atgcagaagg cctgcggtgt cgggggcacg gtgaaggaag ggcggattga gattcaaggc
    47761 gataagcgcg acgacgtcgc ccgcattctg agcaaggccg ggttcaggcc ggtatttgcc
    47821 ggcgggtgag atctcatggc tgagccgctc aagaatagtt tcggggccga tgttccgcgc
    47881 accatcgcga ggatgattgc cgccgtcttc ccccgattcg atcagaaagc gttcgtcagg
    47941 tcgtcgttgg aagggtatga cgcgctcgag ctgatgcccc gcggctggaa aatcgcccat
    48001 cagcttcgcc gctccttgcc ggacgactac gagaaagcgg tcgacattct cctggcgtcg
    48061 ctcgaccgga agccggagcg gaccgttgcc cagggaatgg gcggcttcct gtttcttccg
    48121 catgtgttct ttgtggcgga gtacgggctg gagcatttcg aaatctccat gcgggcgcaa
    48181 tatgtgctga cgcaacgctt caccgccgaa ttcagcattc ggcggtatct cgaacggcat
    48241 caggtggcga cgttggcccg cctcatggaa tggtcggccg actccagcga agatgtgcgt
    48301 cgtttggtgt cggaaggcac gcgcccccgg cttccctggg cttcccggtt acgcgccttt
    48361 caggccgatc cgcgccccgt gctcgcgttg ctcgagcgac tcaaggatgc cccgtcgttc
    48421 tacgtacgtc gaagtgtggc caataacctg aacgacatcg gcaaggacca tcccgctctg
    48481 ctggtggaga cggccaagcg gtggatggtg ggcgcgacgg aggagcgtcg gtggattgtt
    48541 cgtcatgcgt tgcgttcggc ggtaaagcgg gctgatgccg atgcgttggc tgtcttgggg
    48601 tatggagagt cggcgttcct gtcgatccgc aaggggcgtg tgacgcctcc acgggttact
    48661 atcggttcgg ccgtcatgat cgactgtgag ctcgtcaacc gatccgccaa gccgcaacgg
    48721 gtgctggcgg acctgcgcgt gcattacgtc aaggccaacg gcagcaagcg cccgaaggtc
    48781 ttcaagctga aggcggttct gctgcctgcc cgggcatcac tgccattgag caaacgcctg
    48841 ttgctgtcac aactcacgac tcgcacgcac tatcccggca cgcacagggt ggaactcctc
    48901 atcaatggac gctcccatcc cttgggtagg ttcgatttga tggagccgtg agcgatgcat
    48961 acagggttga tgtcattatg accgagtcgg cttttcgtga gacaactctg ccgttgacag
    49021 cctcttctta agagtctagc ctgaagcctc ccacgatcct tgatcgtccg catggttctc
    49081 ttccccgagg tcttcccatg ctgcgtgatt gtggaagagc cgccccagat ttcggcttgc
    49141 ctctcacggt gcaagtctcc tcgcccgtcc ggcgccctgc atcctcgact cgaccaacag
    49201 ttcgtgcggc ggttcgatct accgttaccg tttttacgca gcgggtccct ctacctccat
    49261 catgccgatc attcactgtg acctgacaag caggaggtct tgccatgtac actgatttct
    49321 acggtcttat tcgagcaccg ttcggcacga cgcccgacgc ggacaacctg ttcttgagtc
    49381 cgagccatcg agaggcattg ggctcgattt tgtacgggat caaggagcgg aaaggcgtca
    49441 tccttgtcac gggcgaagtc ggcgtcggca aaaccaccat cctgcgtcgt taccttcaaa
    49501 tcgccgcccc cccgaagcag tcgtctccac atccacagaa aaccgtccat ctctacaacc
    49561 cgaatttgac gttcgaccgc ctgctgacca cgatcctcca gcagttgggg gcccaaccgg
    49621 tcagcgagga gcaggctgag atgctggcgc agctgcatga cttggcgaag gcggagcgca
    49681 acggcggcgg caccgttgtc ctcgtcatcg acgaggccca atgtatgccg gtcgagactc
    49741 tggaaggtat ccggatgttg accaacttgg agacggcgca cgaaaaattg atacagatcg
    49801 tgctcaccgg tcagcctgaa ctggaagcgt tgctggagcg tcatgaggtg cgccatatcc
    49861 gtgagcgcat cgcggttcgg gccaggatcc tgcctctgac cgatacggag agtgtcgcct
    49921 acattcagca tcgcctcgat caggccagcc gtgagcaggt ctcgattttc tctccggcgg
    49981 cgctccgtat cctcgccaaa gccgcgcacg ggattccgag acggttgaac atcctgtgcg
    50041 acaatgcgct tatgaccgga tttcgccgac gagaacccat catctcccgc caggtcgcgc
    50101 aacaggtcgt ggcggagatt gatgaggtgc aggcgcgtcc ggtctggaag agacgcgagg
    50161 cggtcgtgct ggcgtccgtg ctggccttgg catttccggt ctggctctat ctgggatggt
    50221 tcccgagcgg gccatgggaa gggttcggtc aaacagctct ggaggcaccg ccgtcaccga
    50281 aagccatcgg tctatcaggt ggcctgccgg tctcgccacg gaacgcacgc gaggttctgc
    50341 cggttgattc agtgtcgacg gtcgcaacgg ccaatgcttc gcccatgact ctccaacatg
    50401 ccgctccgtt gcccgtgccg cgacccgttc cggtcttgcc tgatctcgtg agggtgagcg
    50461 gacctcctaa atcgtcgcct cagaacgagg ccctgcgcgt acttgacgac aagggcatcc
    50521 agactgccat gagcggcgaa ctggtttcac ccgatgatgc ggagtccggg gagattgccc
    50581 gtctgctggt cgtgctgctg gacgcgggcc gcgtcgtgtt gggaaaagcg caggcgacga
    50641 tcaacaatcc gcgcttggag gacaaggggt tttccggagt ggtgtttcag gcccgtctgc
    50701 gcaaggagtt cctggcgagg accgggcatg acctgcaggc cttgaacgcc gccgccatgc
    50761 cggagcgcgc caagccgctg ctggccaagc tcgccgggat catgcagaaa gcagtgcagg
    50821 acgttcagtc ggacatcaat aagaagggca ttgggttcaa ggggttcatc cccgccacgt
    50881 tcgggacgca ggttgccgcg agtttttcca aggacaccgg gcttaagtta cgacagatcg
    50941 ggccgccgga cgtggatccg agaaatcccg acaacaaacc cgacgagcag gaaacggagg
    51001 ccttgctggt cgtccaaaag agccacccgc gggtgggtga ccatgtcatc tcgcaacgac
    51061 tcgcggatca gagcgtccga gtcgtgctgc cgctctttta cacgaaggcc tgcttgagct
    51121 gccacgggaa acccaaggga gagctggata tttccggata cgagaaggaa ggattcaaag
    51181 agggcgatct cggcggggcc atttcggtaa ttcttccctc tgccgccccg acgacggcga
    51241 gccagcggga gggctgagtc caatgggccg gtcggcagtc taggcgcccg cgtgtttcct
    51301 cggatcgatc acctgacccg tcatcgtgcc ttccacactg gcgagataac attgcgcgac
    51361 cgtctcagcc ggcaggccga tcgatgggtc catgcctctg gcaatcagtg tttccgtcac
    51421 ccagggaggg ctgacgacat tcacgcgcag gcttcgcggc aggtcgatgg cggcggcgcg
    51481 gacgaaccct tccagtcccg cattggccat gctgattgcg cagctgcctt tcatgggttc
    51541 ctggctgagc acgccgctgg tgagcgtgaa tgatccatgg ttggtcacga acggtctgcc
    51601 gatccgcacg agattcgcct ggcccatcag tttgttcttc aggccgatga aataatcgtc
    51661 gtaagtcagg tcgtccagac tcgcgaactt ggcgatcccg gcggtgctca cgactgcgtc
    51721 gcaggtaccg atggttttga agaggctggc gatggagtcc gtcgaagcca ggtcaatctg
    51781 atacgttccc ctcttgtgtc ccaccgtcac cacatcgtgc cgcgtcgaga gtagcttcac
    51841 gacggcggat ccgatggtgc ctgttcctcc gatcacaatc acccgcatgc cgaacctccc
    51901 ttgttcatgt atgaccgagt gtagtcatgg ggctctctgc ggcgcaagag tcgttcactc
    51961 gggactgtcg atgacgtatt gcgctctggc cgagggctct gtggccggct gaatgtggcg
    52021 gcaaggggga atgtccgcct gtaggcgtcg caagagcacg gtgatttcca tgcagtcccg
    52081 tacatgaacg acggaatagg gacctgattg taggcag
//
LOCUS       DAORRQ010000027        51526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_38_length_51526_cov_235.214023, whole genome shotgun sequence.
ACCESSION   DAORRQ010000027 DAORRQ010000000
VERSION     DAORRQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 51526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38_length_51526_cov_235.214023"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PK224_10300"
     CDS             <1..1239
                     /locus_tag="PK224_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRB16170.1"
                     /translation="DAMSAIERDNLSLKGVLPKDYARPGLDKQRLGQLINLVSDIGLG
                     SPADRAKDILGRVYEYFLSQFASAEGKKGGQFYTPSRVVRVLVEMLAPYKGRVYDPCC
                     GSGGMFVSSEKFIEAHSGKLGDISIYGQESNYTTWRLAKMNLAIRGIDAQIAHGDTFH
                     NDKHPDLKADYVLANPPFNDSDWRGELLKDDKRWVYGMPPSGNANFAWVQHFIHHLAP
                     TGIAGFVLANGSMSSNQSNEGEIRKNIIEADLVDCMVALPGQLFYSTQIPVCLWFLAR
                     NKRNGRFRDRRGEMLFIDARKLGTLIDRVHRELSDADIGKIAGTYHAWRGDTSAGPYV
                     DLPGFCNAATLDEILKHGHVLTPGRYVGAEAVEDDGEPFEQKMTRLTKTLQEQQAEAE
                     KLDDAIVVNLQALGFWKDTA"
     gene            1236..2933
                     /locus_tag="PK224_10305"
     CDS             1236..2933
                     /locus_tag="PK224_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16171.1"
                     /translation="MTPEALYDVIAAGETLAVELKGEEKAPLNDRELVETVVCLANRT
                     GAEPGYLLVGVEDDGRITGARPRHGATTDSDRVAALIAGRTRPALSVSVGVVPLQNAE
                     ILAIEIPPTCHPVGTSDGVFLRRARGGDGRPACLPMDIPTMQSLQSDRGLLDPSAQVV
                     TAARWEDLDPLEFERFRRSIRERRGRSDESLIDFSDLELAKALGVVEANGSLRAVRLA
                     ALLLFGREDVLRRLVPTHELAFQVLRGLDIEVNDFFRWPLLRVMEEVESRIRARNREQ
                     ELMVGLLRVGVPDYPERALREAVANALIHRDYQRIGAVHFQWHPERIEISNPGGFPEG
                     VRLDNLLVTAPRPRNPLLADACKRAGLVERTARGIDTIFYEQLRNGRLAPSYARSDAV
                     TVVIVIPGGEANLDFVRLLVTEAQAGRALGLDDLLILNAVWQERSVDTAEAARLTQKP
                     ETETRATLHRLVEAGLVEERGEKRGRTWHLSAAVYRALGDRAAYVRQRGFEPLQQEQM
                     VLQYVEKHGRITRKEAAELCRIGPYQATRLLDRLVKDGRLLRHGERKGAWYERGAKV"
     gene            2930..3241
                     /locus_tag="PK224_10310"
     CDS             2930..3241
                     /locus_tag="PK224_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16172.1"
                     /translation="MSALGTIWLSLKYMSAAHNLTVRGSLNAAIYSLRQPTKRRMITW
                     LDALGRPATLASDKNARVHLYARRVYSQTKAEKFEVATSLPKHRHRQVSVSLKELGYG
                     G"
     gene            3231..4565
                     /locus_tag="PK224_10315"
     CDS             3231..4565
                     /locus_tag="PK224_10315"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRB16173.1"
                     /translation="MAGDDFERTTFGDLISEGSLEIGDGYRAKNEELGGDGPIFLRAG
                     HVTDTHIDFSGVERFHSELESRVRSKLAKPGDAVVTTKGNSTGRTTFVTASMPPFVYS
                     PHLSYWRSKDPTRVEGGFLRYWSKGAEFTGQLAGMKASTDMAPYLSLVDQKRLRISLP
                     PIAQQRSIALILGTLDDKIELNRRMNETLEAMARALFSSWFVDFNPVRANAEGRDTGL
                     PKHLDDFFSDSFEDSELGEIPRGWEIKAFADTIEIVGGGTPKTSVAEYWDGDIPWFSV
                     VDSPTDSDVWVVDTEKKVTHAGVENSSTRVLPEGTTIISARGTVGRIALVSVPMAMNQ
                     SCYGLRGRAEAHGFFTYFSTRELVARLQQHAHGSVFDTITRDTLAGVSVVAPAAALIE
                     EFEKRVAPILHRIRVGLLETRTLAALRDALLPKLISGELRVPEGTIKLGGRA"
     gene            4562..5521
                     /locus_tag="PK224_10320"
     CDS             4562..5521
                     /locus_tag="PK224_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16174.1"
                     /translation="MKHLANLGSQFSISIPPDEDGLVGRECPVPECESYFKIQLGTGL
                     KGETLLCHCPYCGYAAEPDKFFTKAQLEYAQSVVMNQVTGAVLKDLKSLEFNHRPRGA
                     FGIGISMKVSGRPTPIRHYREKQLETDVVCEQCTLRYMIYGVFGFCPDCRVHNSFQIL
                     KKNLELVGKMLAVAETQEQAVAQSLIENGLEDCVSAFDGYGREICRLHAKRSTDLEKA
                     EKVSFQNLEGAKQSTKLLFNLDLAAGLSDDEWKEAVRAFQKRHVLSHKMGVVDEEYIR
                     KSDDTHAVVGRKVNIAIDEVRALSQTIGKLGQWLYNSMEGIRP"
     gene            5622..7925
                     /locus_tag="PK224_10325"
     CDS             5622..7925
                     /locus_tag="PK224_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB16175.1"
                     /translation="MINRLQLLRNVGQFDSVSSAANIPLSRLTLIYAENGRGKTTLAA
                     VLRSLSTGDALPIAERRRLAAQHPPHVILDCSGGPPPTIFDNNAWNRTLADLMVFDDV
                     FIDQNVHSGLAVQTHHRQNLHDLILGAQAVALSTRVQQLVVTIEEHNRELRSRESAIP
                     AADRGPFSVDAFCALPSRADVEEAIEETARALAAAQDQDAIRTTQSFDTLNLLEFDIT
                     AVERVLHHDLPAVDAATLVRVQEHLAGLGADGEAWVADGMRRMPQGQGSEPPSNCPFC
                     AQDLGRSPILQHYRAYFSEGYASLRREIADAIRTVEQAHGGDVPAGFERAVRVAIERR
                     QFWSRFCAVGEVTIDTATIVRDWRAAREAIVAQLAAKQAAPLERMEVSDQTRALVVAY
                     ETHRQTIATINQRLQHTNLAIEVAREHAVTANSTVLAQDLVRLKATKARHEPATAACC
                     DQYLQEKTAKEATERQRDQAKAALEHHRTQVFPGYQTAINLYLARFNAGFRLDSVTYA
                     NTRGGPTCTYNVLINNTPVPVGGAEPALGVPSFRNTLSAGDRNTLALAFFFASLDQDT
                     RLTNKVVVIDDPISSLDEHRALTTVQEIRRLAERVAQVIVLSHNKPFLCRLWEGADAT
                     VRSAIEVARDMAGSTLRVWDVVHDAITEHDRRDAKLRAFLDTGAGDMREVARSIRPHL
                     EAFLRVVCPRKFPPGTLLGPFRNLCRQNLGTPNQLLDQNAIRELDEIVEYANRFHHDT
                     NPAWETETVNDHELRGFVDQALRFVGP"
     gene            7930..11106
                     /locus_tag="PK224_10330"
     CDS             7930..11106
                     /locus_tag="PK224_10330"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRB16176.1"
                     /translation="MNQSFTESVVEDAALGWLEALGYQVLHGPDIATGEPAAERSDLN
                     YRDVVLERRLRQALARLNPDLPFEVIEDAFRKLTRSEAPCFIERNRAVHWMLVDGVNV
                     EYRRTDGSIAGAQARVIDFDVPANNDWLAVNQFTVSEGQHTRRPDLVLFVNGLPLAVM
                     ELKNPADEDATIWSAYQQLQTYQAQIPSFFATNAALIISDGVQARIGTLGAGKEWFKP
                     WRTITGAQDAAPTVSELQVVLEGVFEKRRFLDLVRYFVVFEDEGHGKLSKKMAGYHQF
                     HAVNVAVEETLRAAKVVAEHHASDLLGLYQSGHQPGGEAGDRRVGVVWHTQGSGKSLT
                     MAFYAARVILHPTMANPTIVVLTDRNDLDDQLFGTFARCRDLLRQDPVQATDRADLRA
                     KLAVASGGVVFTTIQKFFPEGKGDRHPVLSDRRNIVVIADEAHRSQYDFIDGFARHMR
                     DALPNASFIGFTGTPIEKTDANTRAVFGDYISVYDIQRAVIDKATVPIYYESRLAKLE
                     LNDSERPRIDPTFEEATEGEEVERKEKLKTKWAQLEAVVGSENRLKLIARDLVDHFED
                     RLATMDGKAMVVCMSRRICVELYREIVTLRPHWHAEADDQGSMKVIMTGSASDPIEWQ
                     GHIRNKKRREDMALRFRAPKDPFRLVIVRDMWLTGFDAPSLHTMYLDKPMRGHGLMQT
                     IARVNRVFKDKPGGLVVDYLGLADELKQALATYTESGGTGKTAIDQAKAVAVMLEKYE
                     ICRGLFGSYTTPAGMVRGFDWSPWMTGKPQERLSVLPAAQEHILAQEDGKARLLRAVT
                     ELSQAFALAVPHEETFRIRDDVGFFQAVRAVLAKNIPAEHKTDEELDHAIRQIISKAV
                     VSDEIVDIFAAAGLRKPDLSILSDEFLAEVRGMPQKNLAVELLRKLLAGEIKVRSRKN
                     VVQARSFAELLEQAVRKYQNRAIEAAQVIEEMIGLAKDMRRAHERGERLGLSEEELAF
                     YDALETNDSAVQVLGDETLRTIAREVADAVRKNVTIDWTVRENVRAQLRVIVKRILRK
                     YGYPPDMQEKATQTVLQQAEVLCGEVAA"
     gene            11607..11918
                     /locus_tag="PK224_10335"
     CDS             11607..11918
                     /locus_tag="PK224_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16177.1"
                     /translation="MATSEILPVLHEAKETLESAGLDVSITDLDEETRCLGFYVNDVG
                     LFFTPAEDGLTVCFMARRFTGKEQGYESGSSTSGPRQNRSVVWLRRPYSVSSGPAGPT
                     Q"
     gene            12026..12454
                     /locus_tag="PK224_10340"
     CDS             12026..12454
                     /locus_tag="PK224_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16178.1"
                     /translation="MTNDSEALLGLRVALREGQDFGLLPCETEAGRRFQVMVGELAAP
                     VLRELVTLLCMEGLATHLHMALDEATPYIGLEITEPSTTLWIFPSATTNEITTSVQGG
                     LYPDYASDRHVCYRALMPETLERVLVEQLRLVLCPTFPII"
     gene            12857..13330
                     /locus_tag="PK224_10345"
     CDS             12857..13330
                     /locus_tag="PK224_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16179.1"
                     /translation="MSTSTHPPYQYLRQKIREWQAGDLLQPVDQRLRQFRTITPVALL
                     PLVRDLQQALEAEGLRATVREMSLELSFLSLTVDDFDIEVSFGPCDAPDACQMTTCRM
                     GSPEPAAIRLLAYRDLETDVAGVMGQLEEAVLCALGPRRTTTRNPIGEPPAGPRS"
     gene            13393..13827
                     /locus_tag="PK224_10350"
     CDS             13393..13827
                     /locus_tag="PK224_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16180.1"
                     /translation="MQLCEAYTTLQNKLQQYEETGRLTPRPDAMRSRFLAFAEADLMP
                     LTRRLATILTQAGIPAYTRAQLEEEAMWFGLFLNETWSVGIYVQPLDAASMQLSLQFN
                     VDSEAEEHHILLYRKCTRATFATTLERAVERLLMQTWEERRR"
     gene            13881..14333
                     /locus_tag="PK224_10355"
     CDS             13881..14333
                     /locus_tag="PK224_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16181.1"
                     /translation="MPHAPHTDYAHLKAHLEAWPHRQQTPESASQEAFSRLIEDRLLP
                     ELQRLAGVLRDAGMDCTVFREETETTGVGLRIEDLQATIGLLRGEQDTYIRACVARDQ
                     RPNGQIEWFIPYTRIYQGLLERELQAAVVRLLKSRGAGSAGTHRGPTP"
     gene            14435..14635
                     /locus_tag="PK224_10360"
     CDS             14435..14635
                     /locus_tag="PK224_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16182.1"
                     /translation="MNDSDALWNVRLALRDDQPFGFLPYEQETDFVEHLQLALYSLKW
                     LIPYHHIANSGLEPEILSVLMS"
     gene            complement(15202..16557)
                     /locus_tag="PK224_10365"
     CDS             complement(15202..16557)
                     /locus_tag="PK224_10365"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HRB16183.1"
                     /translation="MPLHRHDPNIALSKTQALVADRTVSAIFEAAFLFDDILIRADIL
                     ERESDDSWHLIECKSTSRAKEAHHWDIALQAYVLQGCGMNVRRYSVMHINRGYRRGLV
                     LNVERFLRVTDVSSKIQAVSKQIPQQLKTMKAVLMAPHPPKVEPGPHCWQPYPCEFWA
                     HCTHKKSSEWVGYLPEGERQIPKLIARGVQTVDQIPDGFYLSTIQWHAVRQEKWRSPH
                     LQTFMKRLRYPIHYLHIEAAWYGMPLFPGLRPYERVPFQWTTLRESKSGTVNREEWVD
                     LSQERSPILAFVESLVGRLGTKGAIVIYSDAVLWTLKSVSQRLSVESPYKQLVKKLLV
                     RCVDVREIIRSKYYDPRIPCGVYEAIPRRDTSIQQIVGVLSDPQRYELLSTLDIEWAS
                     RSYRKITTRGMADVAVRSFRDQVLARSHQAVQALYAVRQLMTAPSASNRSRKARLHGV
                     R"
     gene            complement(16791..18305)
                     /locus_tag="PK224_10370"
     CDS             complement(16791..18305)
                     /locus_tag="PK224_10370"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HRB16184.1"
                     /translation="MSPQDKKAKRFDLDRLQSFTHNHTRYTHLLDLDGIKVWSVSWGE
                     RWKFRAVTEAPPANCPGCQNSMSLLKYGQKEISFDDLPLRGSPVRVKLSRQRYKCDQC
                     KLLFIEPVEVLSPEWRATWRLVMAIAHASLHRPFLQLAKEYGMHERTIRRIAHSFFRV
                     LDSMAQLPTPQWLGIDEVKVHGIPRVVFTNLKKRLTYHMLPDTQEKTIRAFLRSMPGR
                     EAVEVVCMDMCDRYRKIVRELLPDARIVVDKFHLVQLGMKAVDQVRREVKRKRRVKVK
                     YDTRIVRKRRRNLTPHDREALSYWRVAVPQLADVFELREAFCDIWDAPNKTQARALYR
                     AWKKKSLQVLPQAFRPRINQIQKWDTEVFNGLCVKVTNGYAERVNGIVNHLNRTTRRL
                     GFDALRAKLLYVYGYPKSPLSQKPKAEWSDPTYELDEYERYLAEQSVLFESPSVGFSM
                     HDIAEAYLARVELDADLHGQPVRYPTSPDDIEMMIAVGMMRILPESSEDAGLAA"
     gene            18647..19711
                     /locus_tag="PK224_10375"
     CDS             18647..19711
                     /locus_tag="PK224_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16185.1"
                     /translation="MVSRYTRVRKGPFTCATYQDGTQWIYVRVYHAHDEYTLGRYPTQ
                     DDAQLAYDEFSLQQALQRFPELTPEQQQLLQQTITAHPRVAKPVARMVRNWRPIELGP
                     DFRHRKPSSPRRHASAEKPIRGIRQVFLKNGEPRWILALTHNHDVKCRRFSSLQDAED
                     YYREVQVQRALAAFPPLTDDQVKELTALQKEPLLRPVVDLLINCWMLAPRLKPKLNRR
                     QGALPFQSGRPQHQSQKPTGPPPDPGITFADYAYRWFLATADRWSPASQDIVRSLCTA
                     HLVPAFGPTPVRDLRTEMIAEWLQGQLKQYSLQHCCHMRTQLVSILYAAVADGLIASN
                     PAVGPGCMLPFKALHNQRAR"
     gene            19731..20990
                     /locus_tag="PK224_10380"
     CDS             19731..20990
                     /locus_tag="PK224_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB16186.1"
                     /translation="MTTTKHPKDSKDRGICERKLSNGTIIYYIRLYHNGKPVVFGRFH
                     SKAAARKVYAKAKVEQDEGRFDPTKYQVRGPAPKKLQGPLIFKDYAEQWVKEHRATYK
                     PSTLRGYRSILRSRFYPTIGNVPMAALNRQMIKDIILDDMDRRTADGEELKDRTKRGV
                     YQVIKRVLKSAFLEELISRNHADDLHEFLRSPAKFLVTPLTEEEEKQFLGAVQRYAPQ
                     WYVFFFLLLRTGLRIGEALALQPADIHFEKRCISIVRNWTFGGIQTTKSDEGRCVDLA
                     RKLAEVIENHITEQRLEAARHWTQPPIWLFPGKGEHPISQDHVRTNVFRMVLRKAGLR
                     RFRIHDLRHTFATRLLLKGDRNGITLKYVSEQLGHTSIATTADIYTHLLPQGNIGAID
                     ALDELDDRPNEEGSPGTATPADTEEAA"
     gene            22173..22421
                     /locus_tag="PK224_10385"
     CDS             22173..22421
                     /locus_tag="PK224_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB16187.1"
                     /translation="MNSARTHSPTPLVQALLTAGGAGPLPELLTLSEVCAFLKVKPSY
                     IYSLTSTDRIPFRKFGGTLRFVKAELLQWLDRGRRGPR"
     gene            22489..23703
                     /locus_tag="PK224_10390"
     CDS             22489..23703
                     /locus_tag="PK224_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB16188.1"
                     /translation="MATTRTLEKGCLYRRKNRPTYYVKFSPRKGAKPIHLSTGKTNLL
                     AAAASAQVLIAKYDGKSTNQVKGQKLTIADFCRLPDKHDPTDRGGQFWQYLTANRAGN
                     TRTRYRDILKTQIIPTFGRLRFNEVEPEGIEAWKQRRLLQVQRSTVLKELHCLSAIFR
                     VSRKVYRYTTQNPVGDIEKPTVPKRKKQIPTADEIKAFLDAAGLLTPHYYSSFLTIYQ
                     GGLRIDEARHLEPSDVDEEENVLHIRVKTGWSPKDQEDRDIPLVEPMRTVLLTLKHQH
                     PHARWLLPRGNTRTYTCQRCGGPTTHIGNLRTAILSLATAAGISKRMTHHILRHCNST
                     HNRQLGAKDYEVMALLGQQSTKIHTIYTHAEWRNIVDATARLGVSIGQDGLSAWLSAR
                     AREAEESNRSRT"
     gene            24079..24672
                     /locus_tag="PK224_10395"
     CDS             24079..24672
                     /locus_tag="PK224_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16189.1"
                     /translation="MVMVSFLHSHSLVMFFYCVSLLLVGCSTSSRTLTAFDLVVKTPS
                     ISWAEKQIVRQRGVYLYRLIPENEVDPKRHRAAFLQRKNELLGIAVEQFPIASGSYAN
                     GEIRFVLPEKFEPSTHCFAVVAASGYLIATSAFGSDNKRYLIGLSPYSKRYLGSPGDA
                     SISRGGNMFSTTTDLECAGTLEDFTSKNSQLYSVGTP"
     gene            complement(24931..26610)
                     /locus_tag="PK224_10400"
     CDS             complement(24931..26610)
                     /locus_tag="PK224_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16190.1"
                     /translation="MTGAYLRPLLKVISFVPLLSALGCTGFLESFHRVDEMISHRRCE
                     KPEDCRDILRATQNKLSVVRDPMTLWHYDDRVGYQAILGHQRTALDDFEKVIDLNYPD
                     HVGPYTRARREFGIEQWEAARMYAEAWELYASKGFPLAGEKFSILLSQGFEPAGAYLG
                     LYLSLCETSHCPKSSDLLDKGISLAPRSFRLRVARAIHLLASEEMAKAAPDIEQVMAF
                     TPQFAEGYIIQGSVLVRAQRYEEAVAKYAKALEMKHITSDLYMERAHAFAGLNRFDEA
                     ILDLNQAVQLNKENDSAFALRAALHFMREDRSRAFTDWSSALKLNSTLYGAKLIAFYT
                     DLLKVGANDAELYYRRAEALATLGRFDESLRDVHETLSRNPKHAEAYFVRGLCHRALQ
                     QNSQALADFKNAIQLDPLVEGAHLQLGLLYSELFRGSDPKEWSHFREADRYLLSSGVK
                     QDQENYLRALLYASAGTRNYMQQAIILCSAAVRTEPNNKLYVDALGKLQQADAQLRNQ
                     AFEAAFWTFLGFWAIGAYNGAGTLPDVYKPRTLPQPFCTRKGFPHPYPCFP"
     gene            complement(26936..27466)
                     /locus_tag="PK224_10405"
     CDS             complement(26936..27466)
                     /locus_tag="PK224_10405"
                     /inference="COORDINATES: protein motif:HMM:NF020068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRB16191.1"
                     /translation="MAYQQKSMGPVPMAWGLGILLGFHLALCATPLLAQVGVPLPPKP
                     PQLPVEPPPAQPPALEVPPPAPPVPGEPITGAKIFVKEVRLIGNTTFTSQQLTEVTGP
                     YTNRDVTAEELEALRLALTYYYVNHGYVTSGALIPEQTVTDGILTVQVVEGRLAEIKV
                     EDTRWFKSERSFQRGH"
     gene            complement(27863..28801)
                     /locus_tag="PK224_10410"
     CDS             complement(27863..28801)
                     /locus_tag="PK224_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRB16192.1"
                     /translation="MSSGVRLKRWISAGCVVLLMPVFWMGFARASQPPGETGPTESIR
                     GSACYKYGDNETPVQAKRVAIALAQEDAVRLHKVFVQSSSKVKNLQLEEDIVQTASAA
                     MLEQVQVEKEEKHGQEICITIAARMSPVSIDELIKQRINAKEISQQAQAPMVAASAGF
                     GLRVWTNREDGRFSEGEELIIHVQSDRDGYLKLDYFQADGCVVHLVPNLYRGEAFIKG
                     GQTYSFGSATSPEQFTVTGPFGAETIKAIVGVKPFDPSLQGKSTECDDGRAYVQGLQQ
                     GLRGLKVSGVEQALSLTTVSRTVEGYRKDRRPGLSP"
     gene            complement(28881..29633)
                     /locus_tag="PK224_10415"
     CDS             complement(28881..29633)
                     /locus_tag="PK224_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HRB16193.1"
                     /translation="MVDWTAGMVTALGIGYPPSDTINSTQARLLARRAAQAVAYRNLL
                     EAFTAIRVDSTTTVKNYVATSDEIQVKVQGMIEDAKVIQERELEKGGYEVTLQMKLTG
                     QTSQMFAPKDAPAPKRLVRELPPDARPKTAAPYTGLVVDARGMEVRPALVPRILTEDG
                     QEAYSQSYVLAKYRQNQGIAAYVSDPLAAKTHPKVTDNPLYVKALRPAGNSHTDLVIS
                     NAAAQTIHGVKDHFEFLEKGQVIVVVDRSGSQ"
     gene            30170..32755
                     /locus_tag="PK224_10420"
     CDS             30170..32755
                     /locus_tag="PK224_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRB16194.1"
                     /translation="MPDRIFSGKYAVQEEIAKGGMGVLYKALDRTLNRLVAIKLIHEQ
                     FSGDPSFTERFIREARAMARLHHENIVTIFSVEEDRGAHFIVMEYFSGMDLRAKIKAS
                     HSLSVRESVDIALQVAIALAFAHSQGIVHRDIKPANILVDSRGRVKLTDFGIAAALDE
                     ASITSTGQVIGTPEYMSPEQAGGMAVDGRTDLYSLGIVLHEMIVGQTPFRSMPKTSIL
                     TKLLDPEYEIPLIFSSEIPSAVKAIVEDLVRRNPEYRTPRAAILASQLKECLASLPVQ
                     DSDDAPTIVATSPSPGTPRTTRVGATPPPLESPTIPSRQNAGPQIESGPQSPAGQPQL
                     PPISSEARDEATDVLPRTKPASQPPPSAPESKPIRRTYKPVPIAIGVLSLILVLSGLV
                     YVLSNGIRLTTPSADKTEPPASSTTTTTEPANGAGAPHDPASRDSAQLQSKIEQEQRQ
                     LEIDQARVEAKSREAEALQQKEKDQAAQQAKLAEEQRKKTEEAARVTRERQEAEQRKF
                     AQEQKRLEKEKELAAKQAQAAEEQRRKAQEEARVAREQEELEAKRGKEEAETKRLQGE
                     RQRLEKERDAAEEQARLAEAQRKKTEEETRLARERHEAEQQRFRAEQQRMEKERELAA
                     QRALIQEEERRKAEEAARLAKERLEAEQKRLQAESDRLERERELAAQKIRAETEERRK
                     AQEEARVARERDEAEQKQLQADRERLDKERREAASLQAKTEEEERKKRAAQEEERRKK
                     EELSAKLPPDGLLGATASRRQLRDLLDEFKRAYEGQDLEALERLSEMGNDRLGFVRTM
                     FKNYRTIQVSIQSLTVIDDEAAATIIHEVLINNNGETVKPSPILRSARIKIRKTGDRW
                     AKIEW"
     gene            complement(32760..34241)
                     /locus_tag="PK224_10425"
     CDS             complement(32760..34241)
                     /locus_tag="PK224_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRB16195.1"
                     /translation="MSEDLTAPLNTPQSVRVRVCIGTSWSGEQRFVHTFLIGRSADCQ
                     VVVPERVVSRIHAEVRLEAGQWWLVDHHSTHGLYVDGRQVTRVQLKNPVTVELGLGGP
                     ALWLAVGDEAPFVDQSKDAAQSGKKMESVTEIVDRWKRGDTVEESEEARRYREAAKRR
                     ERQQKRWYRGAVAAVVVLLVCALGYALYQRARVDQMTRTAGEMFYTMKQVEIQVSELE
                     EEVVATKRKDLMEKILSKRQRLREMEVQYDRFLGELDIYEGMSEQDRLVFRMARLFGE
                     CEVAMPRDFLSEVKKYIRRWKSTERLHNAIKRARENGFIPTVANEMWTQNLPPQFFYL
                     ALQESGFDSKAVGPPTRMGYAKGIWQFIPQTAEQYGLKPGPLKNVAQYDPQDERFHFH
                     KATKAAAKYIRRIYNTDAQASGLLVMASYNWGEGNVINIIRKMPNNPRERNFWQLLKQ
                     HKVPQETYDYVFYIFSAAVIAENPALFGFDFQNPFAEQVRSRP"
     gene            complement(34322..35086)
                     /locus_tag="PK224_10430"
     CDS             complement(34322..35086)
                     /locus_tag="PK224_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16196.1"
                     /translation="MESSRTGEALTMDQVASLRGKTDAVSHILDSRLRVYIEPLKPLL
                     TPRRLLGRHVGSRDDVNGSDLALTRLKELYREGSHSPFGLPPEFPDTALAQLDSQPAI
                     YRWEYTHRAKSERDFKDATITSPVQWVLSYESGLTLSQVHSMMESRMERKTEALRQFV
                     VNALVMQLLMKSQPNLVQLLAALRYQIDMKPLPGLGALSCVTITAPLASFRPADDVLL
                     MATRFSGVPAFVELVSLESIERFEDPLRTQLQQALA"
     gene            35311..37023
                     /locus_tag="PK224_10435"
     CDS             35311..37023
                     /locus_tag="PK224_10435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRB16197.1"
                     /translation="MTDQVFSGKYIVQEVLAKGGMGVIYKALDRSLNRIVAIKVVHEH
                     LSGDPSFTERFLREARAMARLDHENIVTIHAVEEERGTPYIVMEYLSGSNLRVFMQAR
                     TPVPIRTSVAIALQVAEALSYAHDQGIVHRDIKPANILVDSHGRVKLTDFGIAAALDE
                     VAITTVGQVLGTPEYMSPEQASGDKVDGRADLYSLGIVLYEMVVGHHPFGNLPKTVIQ
                     SQLLDTTQEIRLAFPDTIPSLVKAIIEDLVRRDRDYRTPTARLLVSQLQECLQTLPPP
                     PEPTEEERTIFVTPHPERQTEQRMPPPVSSGSPYRPSTPLPPPPLGTPKPSKGADHVV
                     SVPSGPEDKTDVHMQHPAQRKPESPHVPQPFWQRYNVLPLVAIASVGVLALGGILLYV
                     NVVDQPQPPELPKKEEPLVNPPGPPADRETGDQDRVPIEETSEPDRKVVPSPPPQPTS
                     PKGTSRPSTRPKTIAPSRQQLDQLLSDFQTAYERQDLSSLQRLSDISPDRQMFLDMMS
                     NNYSIIRVSIQNISVKNDQATATLIHEELVDKNGEPVTPDRILRSVRLTVRKDGDRWS
                     KVLW"
     gene            complement(37657..39072)
                     /locus_tag="PK224_10440"
     CDS             complement(37657..39072)
                     /locus_tag="PK224_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRB16198.1"
                     /translation="MKLKKMPLSAALVIPDSLKQAHRVHEVPCAGSYSVPIGLELENA
                     LIGAFSQVFDSVDTITEKKQAAGNYDVVIEVAEPELEIDGHCLTRRFLYMTGPFYLFF
                     DPTDRFEGQGVLRATVTDRNGQILQEEAFKSQRVTKDSMFATSSDRTNAAAAVLRDSL
                     VDTVMQMTRGVVGSPQMVAYARRVMKPAGEKGQRPVAQIVPPSDVDVPPLSASKPQHN
                     RFAVVIGVEQYRQGLASVEFASHDARVMRDYLTKTLGFPEENVAMLVNDQAAKSDIEK
                     YIERWLPNRVDAGSSVLVYYSGHGAPNPITQEGFLVPYDGDPTFLEITGYSLKRLYDQ
                     LGKLPAKDIVVMLDSCFAGTGERSVIAKGMRPAVINVENPLLAGEKTVVLTAGTGAQT
                     SSTYHQKSHGLFTYFLLKGLQGAADTNQDNKIDLAELYSYLKPQVQGVARREFNNDQA
                     PQLLGNPDVLRKGMPLLEFVR"
     gene            complement(39203..39547)
                     /locus_tag="PK224_10445"
     CDS             complement(39203..39547)
                     /locus_tag="PK224_10445"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRB16199.1"
                     /translation="MGQDNSVTVLYPNRFAQDGSISAGKELIFPDDKLRSIGIVLRVV
                     PPPGAAKATERIKLIAVTHKTDFIKDQFKEGIFQVYDGKDTGLITDLLKALSALDDSE
                     WAESTIAYEVNR"
     gene            39690..40856
                     /locus_tag="PK224_10450"
     CDS             39690..40856
                     /locus_tag="PK224_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011363043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HRB16200.1"
                     /translation="MNHGKLVFAQLMQHLPLTTFRRCVTRYRGERKVQLFSCLDQFLC
                     MAFAQLTFRESLRDIEACLRAQRSKLYHLGIRSTIARNTLANANGVRDWRIYADFAQS
                     LIGIARPLYVDEPFGVDLKESVYALDTTTIDLCLSVFPWAPFRSTKAAVKLHTLLDLR
                     GNIPAFIHISDGKMHEVNILDQLLPEPGAFYVMDRGFLDFERLHRFHEAGSFFVTRGK
                     SNLKAQRRYSHAVDRATGLICDQTVVLTGFYSHKGFEAPLRRIRLKDPATNKTLVFLT
                     NNFTLPAFTITELYRCRWQVELFFKWIKQHLRIKAFFGTSENAVKSQIWIAVSVYVLV
                     AIVKKRLKLSTTLYEMLQILSLTMFERIPLDQLLAQAVRDDADHIADKQLILFE"
     gene            41212..42783
                     /locus_tag="PK224_10455"
     CDS             41212..42783
                     /locus_tag="PK224_10455"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HRB16201.1"
                     /translation="MTTHHPTSSVDVVLTAGESRPLGRPAQDIPFRMLLMGNFSGRES
                     SPEGAGRRPPITWRPLRVDRDNLQTMPGKLSATLEGPLLGENTPPITLRFTDLDDFHP
                     DRLVCQIEPLQKLSDLRRRLTNPVTFSAAADEVREWTQPPSPTEARSVGDGAPPHQAG
                     VTGAPMTSLLDQVLEQAPISVADLRLTEWQSYLQSLIQPLLVPKEHPLEKELVAQVDE
                     AMSQILRTLLHHPTFQELRTAWLGLSFLVDRLETDQPVQLYLLDLPKSDLSADLLRSD
                     DLQATQLYRLLVQETVHTFGAHPWAVIGGVYTFDRTTEDVQMLERIAKVSQEAGAPFL
                     AAASPCMVGCSSFGSMPDPDDWQSPTAHQESQQQWERLRRMDQASYLGLSLPRFLLRL
                     PFGPETEAISAFEFEEMAGIPDHEHYCWGNPIFASLLLLGKAFLEESWHLRPGSVQDI
                     DGLPLHVYRDETGDSVTTPCAEAWLTEQAAERLLEAGLMPLLSYKNQDRVRLVRFQSI
                     ALPLKPLLGRWSEAG"
     gene            complement(42805..44814)
                     /locus_tag="PK224_10460"
     CDS             complement(42805..44814)
                     /locus_tag="PK224_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRB16202.1"
                     /translation="MRTSPQHIRAKLRNGLTALLMGMLVGCVSEQLDGTSKLELKDPR
                     SADPFDVVDCLLPGQIRQLGTRVTYLTDRRPLRTTAEDCAIRGGEYVALDRADYGTAL
                     KVWMSAAEGGDRDAQYYVGVLYEKGADGQPNYIKAASWYRQAAERGVSRAAVNLGRLY
                     DQGIGVEKNSVEAGKWFAKASGMREGSLVDVGSGSSSAPAGAPQVQLDIVQQELEQTL
                     SRLAERTSLLDDERRKLQQLKGEGRTPDNRPASTAGDVITNQERLIKARESEVAKLQM
                     QVLSLKEAAQKQASLIRQSPSQDLGLVGPSLQIVDPPVILTRGVRVEQGRIPVVIQPG
                     RPMRLTGRVIAQAGLRSLTANGASVVVDEQGTFSLPVQATQGITGGVHIDLVAIDGQD
                     KQTGLKLIVRSGATALGEEESITSISRAGQYHALVIGNDHYRQWTPLNTAIADANAVA
                     KVLQDQYGFHVRLLKDSSRKEILKALNDYRKTLSERDDLLVYYAGHGFLEPEIDRGYW
                     IPIEGDLLDNTEWIEFPAVTDLLELIPAKQVLVVADSCFAGKLVRSAMARLNPEVQDQ
                     ARQSLLKTIADRRIRTTLTSGGAKPVLDDGGAGHSIFAAAFLGVLSENKSALETERLF
                     WTVRARVAEAAERMKVEQIPTYGPIHMAGHEGLGDFVFVPARTRP"
     gene            44985..46364
                     /locus_tag="PK224_10465"
     CDS             44985..46364
                     /locus_tag="PK224_10465"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HRB16203.1"
                     /translation="MMHQQSQGRKRNFILTISLGIGLLQFVQPMVSTAQVLNQRVNDL
                     LANNCTGLGANVVGGNALLLGPNLNALCQFPNTGAASSSGGGAASVQGSAASILNRVL
                     LQRLDEIDEEEGQEHKRSSSLMFNPIGSLMSGFVGNQSVSSPFYAATTGGGGSAASFN
                     TGRRSRWNGVGFFASGLVESLNRDITTFQDGYKSNILGITAGADYRFSKKLVTGLALS
                     YSNTDGSFRSGGDFSTNSYGGILFASYLPTDKTFVQVTGGYQRNKYLVARTASADSRT
                     AGGTTGGNIVNGIASSNSNGDVFNLGLMTGYDHPIGRFTIGPRLGVNYSHTQIHNYRE
                     TGGTGIELKYDDQSINSMQSVLGVQGSAAFSTGFGVLVPQFNADYIHEFANSQRFIGV
                     QFAEDLRATPTRFTFQNDVPVRNYFNLGTGLVMVLPNGWQPFVNFRAMVGNEQFNNYA
                     GTFGLRIEL"
     gene            complement(46416..47054)
                     /locus_tag="PK224_10470"
     CDS             complement(46416..47054)
                     /locus_tag="PK224_10470"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HRB16204.1"
                     /translation="MGGGRTPGPIGSDTRPLHGPFDPHFPIGGFGTPGPLGLNEGGTT
                     GPRLTGWPRQISWDEFKDVATRPAGETEDAQIDTQTLQPERVGVAREQGQFRLGNFTV
                     KLSVIRDNSWVVANQKSDTLLAHEQGHFDITGLVARDLVKALGALRVSTTDELQREVS
                     RLFEAYDAWAKSLSKQYDDETNHGRNAKSQSDWESRLRTCLQQGTSLGAPPR"
     gene            complement(47223..47756)
                     /locus_tag="PK224_10475"
     CDS             complement(47223..47756)
                     /locus_tag="PK224_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16205.1"
                     /translation="MQRDTAKWINQDLMSQGYHKALEGMSMVREYDLAPDLVLYRFID
                     TTNGSPETGANGPWWLEYEHFQKIKHFALQHGYSLSYAARLFAAILYEYSEVNGYVRA
                     RVLQPLRCWKGRGKQVRSAGKDPRDLPTMTPMQSIHEIYQLYVPGIIRGGSLFGTAFK
                     FLDFTPLPVSKGWPEVR"
     gene            48006..48293
                     /locus_tag="PK224_10480"
     CDS             48006..48293
                     /locus_tag="PK224_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HRB16206.1"
                     /translation="MHVITRRRLIEFAHKHPPARPPLLHWFAIVRKTDYSSFADLRIT
                     FPSADQVGQFTAFNIGGNKYRLIVAIHYNRKKVYIRHVLTHAEYDRGTWRK"
     gene            48297..48752
                     /locus_tag="PK224_10485"
     CDS             48297..48752
                     /locus_tag="PK224_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB16207.1"
                     /translation="MLAKYIHEANAEYRRIRKRVPLGVLHSVEEYDHAVAVLDDILDE
                     IGQDENHSLTDLAEMLALSIESYEEVHVPVPDSSGPQILRYLMEEHGLNQSDLLEIGS
                     QGVVSEILSGTRELNVRQIARLAGRFGVSPALFIPTLKAKNRAMARKRQ"
     gene            complement(49066..49608)
                     /locus_tag="PK224_10490"
     CDS             complement(49066..49608)
                     /locus_tag="PK224_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16208.1"
                     /translation="MAGYFEQHDPLFFRPQLSVAFPRTGLRIEGLVSQGKISAVLSTA
                     ERSKLSFDKKYVVDKYVLNKGTSKTYAGIFQRQAARGSIPWILGPASLIPVVGTAITV
                     ATSTIDGLMRLADSGSVSASQLVVLMADGGAFIKTWFLEKHPEHGELLVTMVFYTITV
                     GTENRIHGIYSDKIALVVRE"
     gene            50068..50298
                     /locus_tag="PK224_10495"
     CDS             50068..50298
                     /locus_tag="PK224_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HRB16209.1"
                     /translation="MERIVNLHIEKLPEGVYLATSDAIQGLVVQGRTIQETIEIARDV
                     AKKILEGKQEREGEPPLPPARDEFDYPLIVNA"
     gene            50298..50522
                     /locus_tag="PK224_10500"
     CDS             50298..50522
                     /locus_tag="PK224_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRB16210.1"
                     /translation="MGRLAGFKYREIVQRLKVLGFQFDRQAAGSHEIWFNPSIHRYTT
                     IPNHPGDMPEGTLRAILKQAGITPADFLDT"
     gene            complement(50589..>51526)
                     /locus_tag="PK224_10505"
     CDS             complement(50589..>51526)
                     /locus_tag="PK224_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02270 family protein"
                     /protein_id="HRB16211.1"
                     /translation="KALVSGLGWIPFQQVAPHIQRLLTAESSQQKGIGLAASAAHRQD
                     PCAALNDALNSIDLLLKARALKSVGELGRKDLLPFLRTNLTEQDTACRFWAAWSGALL
                     GEPAAIQVLQRFAETGDARAGKACATALRRMPLDQAKDWQRHLACSTSRLRMAVQAMG
                     VIGDPANIPWLIQQMADPVLARVAGESFTFITGIDLAYDDLDTDKPEGFEAGPTENPE
                     DENVDMDPDDDLPWPNLQLIEKWWFTHRVLFTNGTRYLCGQPMTIESLNQVLRTGKQR
                     QRIAAAIELAMRQSGQPLFNTSAPGFRQQALLGLQ"
BASE COUNT        11618 a        15142 c        13848 g        10918 t
ORIGIN      
        1 gatgcgatga gcgccatcga gcgggataac ctctcgctca agggtgtctt gccgaaggac
       61 tatgcccgcc cgggtctcga caaacaacgg cttggccagc tcatcaattt ggtcagcgac
      121 attgggcttg gtagtccagc tgatcgagcg aaagatattc tagggcgggt ctacgaatac
      181 tttctctcac agttcgcgag cgccgaaggc aagaagggcg ggcaattcta tacgccctcc
      241 cgcgtggtcc gggtcctggt tgagatgctc gctccctaca agggtcgggt ctacgacccc
      301 tgctgcgggt ccggtggcat gttcgtgagc agcgaaaagt tcatcgaagc ccacagcggc
      361 aagctcggtg acatctccat ctatggccag gagtcgaact acaccacctg gcggctggcc
      421 aagatgaacc tcgccatccg gggcatcgat gcccagattg cccacggcga cacgtttcac
      481 aacgacaagc acccggatct caaggctgat tacgtcttgg cgaatccgcc cttcaacgac
      541 agcgactggc gcggcgagtt gctgaaggac gacaagcgct gggtctatgg catgccgccc
      601 tcgggcaacg ccaactttgc ctgggtccag catttcatcc accacctggc tcccaccggg
      661 atcgcgggat tcgtgttggc caatggctcc atgtcgtcca accaatcgaa cgagggagag
      721 attcgcaaga acatcatcga ggcggatctg gtcgattgta tggtggcgct gccggggcaa
      781 ttgttctact ccacccagat tccggtctgc ctctggttcc tcgcgcgcaa caaaaggaat
      841 ggccgattcc gggaccggcg cggggagatg ctctttatcg atgcgcgcaa gctgggaacc
      901 ttaattgatc gggtccatcg agagctgagc gatgccgaca tcgggaaaat cgccggtacc
      961 tatcacgcat ggcggggcga tacatcagcc ggaccatacg tcgatttgcc agggttctgc
     1021 aacgcggcca cactcgatga gatcctcaag cacggccacg tcctcacccc tggccgctat
     1081 gtcggcgcgg aggctgtcga agacgacggc gagccgttcg agcagaaaat gacgcggctc
     1141 accaagacct tgcaggagca gcaggccgaa gcggagaaat tagacgacgc catcgttgtc
     1201 aacctccaag cactcgggtt ctggaaggac acggcatgac gcctgaggcc ctgtacgacg
     1261 tgatcgccgc cggggaaact ctggcggtgg agttgaaggg agaagagaag gccccgctca
     1321 atgaccgcga gctggtcgaa accgtggtgt gcctcgcgaa ccgcaccgga gctgagccag
     1381 gctacctgtt ggtcggtgtc gaggacgacg gacgcatcac aggcgcccgc ccccgacatg
     1441 gcgcgaccac cgattccgac agagtcgccg cgctcatcgc cggccggacg cggccggccc
     1501 tctccgtcag tgtgggagtt gtcccgcttc agaacgcaga gatcctggct atcgaaatcc
     1561 cgcccacctg tcaccctgtc ggcacgagtg acggggtgtt tctccggcga gcacgtgggg
     1621 gggatggccg ccctgcctgt ctgccgatgg acattccaac catgcaatcc cttcagtccg
     1681 atcggggctt gttggaccct tccgcgcagg tcgtcacggc cgcacgctgg gaagacctgg
     1741 atcccctcga atttgagcgg ttccgtcgca gtatccgaga gcggcgtggc cgcagcgatg
     1801 agtctctcat cgacttttcc gatctggaat tggccaaggc cctcggagtc gttgaggcca
     1861 acgggtcgct ccgtgccgtc cggctggctg ccctcctgct gttcggcaga gaagacgtct
     1921 tgcggcgcct agtccccacg cacgagctcg cctttcaagt cctccgtgga ctcgacatcg
     1981 aggtgaacga cttctttcgc tggccattgc tccgcgtgat ggaagaggtc gagagccgca
     2041 tccgggctcg aaaccgcgaa caagagttga tggtgggtct gttgcgagtg ggcgtgcccg
     2101 attatccgga gcgggcgctc cgcgaagccg tggccaatgc gctcattcat cgcgactatc
     2161 aacggatcgg cgccgtccat ttccagtggc atcctgagcg catcgagatc agcaaccccg
     2221 gcggcttccc ggaaggcgtg cggctcgaca atctgctggt gaccgctccg cgcccccgca
     2281 accccctgct ggcagacgcc tgtaaacggg ccggcctcgt cgaacgcaca gcccgcggca
     2341 tcgacacgat cttctatgaa caacttcgaa atggccgcct ggcaccgtcc tatgctcgaa
     2401 gcgacgccgt caccgtggtc attgtcattc ccggtgggga agccaatctc gacttcgtgc
     2461 gcttgttggt cacagaagca caggcaggcc gagcactggg gctcgatgac ctgctcatcc
     2521 tcaatgcggt gtggcaggaa cggagcgtcg atacggcgga ggcggcccgg ttgacccaaa
     2581 agcccgaaac ggaaacacgt gccaccctac atcggctcgt agaggcggga ctcgtggagg
     2641 agcggggaga gaaaaggggg cggacgtggc atctctcagc cgccgtctat cgggccctcg
     2701 gggatcgggc cgcctacgtg cgccaacgag gcttcgagcc actgcagcag gagcagatgg
     2761 tcctccagta cgtggaaaag cacggccgga tcacgcgcaa agaggcagcc gagttgtgcc
     2821 gaatcggtcc ctatcaggcg acccgcctgt tggatcgcct ggtgaaggac ggccgcctac
     2881 tacgccacgg cgaacgcaaa ggggcctggt atgagcgagg agcaaaagta tgagcgcgct
     2941 tggaacaata tggctgtcgc tcaaatatat gagcgccgcg cataacctca ccgtccgtgg
     3001 cagccttaac gccgccatct atagccttcg acaacctacg aagagacgga tgatcacatg
     3061 gttagacgct ctcggaagac cggcaacttt ggccagcgat aaaaatgcac gcgtgcattt
     3121 atatgcgcgg cgcgtgtatt cgcagaccaa ggcggagaag ttcgaggtgg ccaccagtct
     3181 gcccaagcat cggcatcggc aggtttccgt tagtctgaag gagcttgggt atggcgggtg
     3241 acgactttga gcgaaccacg ttcggcgacc tcatttccga aggctcgctt gagattgggg
     3301 atggctaccg cgccaaaaat gaagagcttg gtggggatgg gccgatcttt cttcgtgccg
     3361 ggcacgtaac cgatacgcac attgatttct ccggggttga gcgtttccac tcggagcttg
     3421 agtcgcgcgt ccgatccaag ctcgcgaaac ctggcgatgc tgtagtcacg acgaagggca
     3481 atagcacggg ccgcaccaca ttcgtaactg cctcaatgcc tccatttgtc tattcgccgc
     3541 acctgagcta ttggcggagc aaagatccga cccgggtcga aggcgggttt cttcgctatt
     3601 ggagcaaagg agcagaattc acgggtcagc tagcaggtat gaaggcgtca actgacatgg
     3661 ccccatacct cagccttgtc gatcagaaac gtctgcgaat ttcgctgccc ccgatcgcac
     3721 agcaacgttc catcgccctc atcctcggca cattagatga caagatcgag ctgaaccggc
     3781 ggatgaatga gacactggag gcgatggctc gtgcgctctt tagttcgtgg tttgtggact
     3841 tcaaccccgt ccgggccaac gccgaaggcc gagataccgg cctgcccaaa catctcgatg
     3901 atttcttctc ggattcattt gaggattcag agctggggga gattccgagg ggatgggaga
     3961 ttaaagcatt cgcggacact atcgagatag tcggtggtgg aacacccaag acctcggtcg
     4021 cggagtattg ggacggcgac atcccatggt tctcggtcgt ggattctcca accgactcgg
     4081 acgtttgggt tgtggatacc gagaagaagg tgacgcacgc tggtgtcgag aactcttcca
     4141 cgcgcgtgct ccctgagggc acgacgatca tatctgcacg cggcacagtc gggcgcatcg
     4201 ccttggtcag cgtacccatg gcgatgaatc aatcttgcta cgggctccgg gggcgtgctg
     4261 aagcgcacgg cttcttcacc tacttttcga ctcgggaact ggtcgctcgc ctccagcagc
     4321 atgcacacgg ttcagtgttc gacacgatca cgcgcgacac actcgctggc gtatctgtgg
     4381 tggcgccggc ggcagcgttg atcgaagagt tcgagaagcg agtcgcccca atcctccatc
     4441 gcatccgcgt ggggttactg gaaacccgca cgctcgccgc tctccgcgac gcgctgttgc
     4501 caaagctgat ctcaggcgaa ctgcgtgtgc cagagggaac aatcaaactg ggaggccgcg
     4561 catgaagcat ctcgcaaatc ttggctccca attcagtatc tctattccac ctgatgagga
     4621 tggcctagtc gggcgggagt gcccagtgcc cgagtgcgaa agctacttca agattcaact
     4681 cgggactgga ctgaagggtg aaactctact ttgccattgt ccctactgtg gctatgcggc
     4741 tgagccagac aagttcttta cgaaggcgca gttggaatat gcccagtctg tggtaatgaa
     4801 ccaagtcaca ggcgcggtat tgaaagattt gaagtcgcta gagttcaacc atcggccacg
     4861 cggggccttc ggtatcggta tcagcatgaa ggtctcgggg cggccgacgc cgattcgcca
     4921 ctaccgtgag aagcaactcg agacggacgt ggtctgcgag cagtgcacgc ttcgctacat
     4981 gatttatggc gtgtttggtt tctgcccgga ctgcagggtc cacaattctt ttcaaattct
     5041 gaagaagaac cttgagcttg tgggaaaaat gcttgccgtc gctgagacgc aggaacaggc
     5101 ggttgcgcag agtcttattg aaaatggact ggaggactgt gtatcggcgt ttgatggtta
     5161 tgggcgggag atctgccgac tccatgccaa gagatcgacc gatctggaaa aggcggaaaa
     5221 ggtttccttt cagaacttag aaggcgcaaa gcaaagcacg aaactgcttt tcaatctaga
     5281 cctcgctgct gggctttccg acgatgaatg gaaggaggcg gttcgagctt ttcagaaacg
     5341 gcatgttctg tctcacaaga tgggcgtcgt ggatgaagag tacatccgca agtcggacga
     5401 cactcatgcg gttgttggcc gcaaagtcaa cattgctatt gatgaagtgc gagcacttag
     5461 ccagaccatc ggcaaacttg ggcaatggct gtataactca atggaaggaa tcaggccgtg
     5521 attacatcga ggtattccct gcgagacacg ctcccaccta agctcatata cggcgaactg
     5581 cacgtgaagg acgccgagcc gttcccccag tgggtcggac catgatcaac cggctgcaac
     5641 tcctccgaaa tgttggtcag ttcgattctg taagcagcgc ggcgaacatc ccgctctcgc
     5701 ggctcaccct catctacgca gagaacggca gaggcaaaac gaccctcgcg gctgtgctgc
     5761 ggtcgctgtc cacgggcgat gccttgccga ttgccgaacg acggcgtctt gctgctcagc
     5821 atccgcccca tgtcattctc gactgcagtg gcggcccgcc gccgacgatc ttcgacaaca
     5881 acgcatggaa ccgcacactc gcagacctca tggtgttcga cgacgtcttc atcgaccaga
     5941 acgtgcactc cggtttggcc gttcaaacgc accatcggca gaatctgcat gacctgattc
     6001 ttggcgccca ggcggtcgca ctaagcacgc gggtccagca actcgttgtg acaattgaag
     6061 agcacaatcg cgagctgcgg tctagggaga gtgcaattcc agctgcggac cgtgggccgt
     6121 tctccgtgga tgccttctgt gcattgcctt cacgagccga cgttgaggag gcgatcgagg
     6181 agacagcgcg ggctctcgcc gcagcacagg atcaagacgc gatccgcacg acgcaaagtt
     6241 tcgatacact gaatctgctc gagttcgaca tcactgcggt cgagcgagtc ttgcaccatg
     6301 acttgcctgc cgtggatgcg gctaccctgg tgcgtgtcca agaacatttg gctggcctgg
     6361 gggctgatgg tgaagcatgg gtagctgacg ggatgcgccg aatgccgcaa gggcaaggca
     6421 gtgagccgcc cagtaattgc ccgttctgcg ctcaggatct cggaaggtcg ccgatcctcc
     6481 agcattaccg cgcgtacttc agcgagggat atgcatctct gaggcgggag atagcagacg
     6541 ccatcaggac agtagagcaa gctcatggcg gcgatgtgcc tgcaggattc gaacgtgcgg
     6601 tgcgggtcgc cattgagcgc cggcagttct ggtcgcgttt ctgtgctgtg ggtgaagtta
     6661 ccatcgacac agccactatc gttcgggatt ggcgagcggc gcgggaagcc atcgttgcgc
     6721 agctcgctgc aaaacaggcc gctccgctgg agcgcatgga ggtctccgac cagactcggg
     6781 cgcttgtcgt ggcctacgaa actcatcggc agacgattgc cacgatcaac caacggcttc
     6841 agcacaccaa cctcgcgatc gaggtcgcga gggagcacgc agtaacagcc aactcaactg
     6901 tgctggctca agatctcgtt cggctcaagg cgacaaaggc tcgacacgag ccggcgacag
     6961 ctgcgtgctg tgaccagtat ctacaggaaa agacggccaa ggaagctact gaacgccagc
     7021 gcgatcaagc caaagctgcg ctggagcatc atcgaacaca agtatttccc gggtaccaga
     7081 ccgccatcaa cctctatctc gcacgcttca acgcgggttt ccgtctcgac agcgtaactt
     7141 atgcgaacac acggggagga ccgacctgca cgtacaacgt gctcatcaat aacaccccag
     7201 tgccggtcgg tggtgcggag ccggcgcttg gcgtgccgtc tttccggaac acccttagcg
     7261 ctggtgatcg caataccttg gccctagcct tcttcttcgc ctcgctcgac caagacacgc
     7321 ggttgacgaa caaggtagtc gtcatcgacg atccgatttc gagcctagat gagcatcgag
     7381 ccctgacgac ggtgcaggaa attcgtcgtc tcgcggagcg ggtcgcccag gtcatcgtcc
     7441 tctcccacaa caagccgttc ctgtgccggc tctgggaagg cgccgacgcg accgtgcgat
     7501 cggctataga ggtagcgcgc gacatggcag gttccactct ccgcgtttgg gacgtcgtcc
     7561 atgatgcgat taccgagcac gatcgccgcg atgccaaact gcgcgccttt ctcgataccg
     7621 gcgcgggaga catgcgagaa gttgctcgat cgatccgtcc gcatctcgaa gcgtttcttc
     7681 gcgttgtatg cccacggaag tttccccctg gaacgctgct cggcccgttt cggaatctct
     7741 gccgacagaa cctcggtact cctaatcaac ttctggatca gaacgcgata agagaactcg
     7801 atgagattgt agaatacgcg aacaggtttc accacgacac caatccggcc tgggagaccg
     7861 agacggtcaa cgaccacgaa ttgcgtggct tcgtagatca ggcgctgcgg ttcgtcgggc
     7921 catgagccta tgaaccagtc ctttacagaa tccgtcgttg aagacgccgc cctcggctgg
     7981 ctcgaagcgc tgggctacca ggtgctccac gggccggaca tagccacggg cgaacctgcc
     8041 gcggagcgca gcgacctgaa ctaccgcgat gtggtgttgg agcggcggct gcgccaggca
     8101 ctcgcccggc tgaatccaga ccttcctttc gaagtgatcg aggatgcatt tcggaaactc
     8161 actcggtccg aggcaccctg ttttattgaa cggaaccgtg cggttcattg gatgcttgtg
     8221 gacggagtga acgtcgagta tcggcgcacg gatggttcca tcgccggggc acaagcgcgg
     8281 gtaatcgact tcgatgtgcc cgcgaacaac gactggctcg cggtcaatca gttcacggta
     8341 tcggaggggc agcacacgag acggccggat ttggtgctgt tcgtgaacgg actgccgctg
     8401 gcggtgatgg agctcaagaa cccggccgac gaagacgcca ccatttggag cgcctaccag
     8461 caactccaga cctaccaagc acagattcct tctttctttg caacaaatgc ggcgttgatt
     8521 atttctgatg gggtccaggc ccgtattggg acgctcggtg caggaaagga atggttcaag
     8581 ccctggcgta cgatcacagg ggctcaggac gcagccccca ctgtctccga attgcaagtg
     8641 gtcctggaag gcgtgtttga aaagcgacgg ttcctcgatc tagtgcgata ttttgtggtc
     8701 ttcgaagacg agggccatgg gaagctcagc aagaagatgg cggggtatca ccagttccat
     8761 gcggtgaatg tcgccgttga ggaaaccctt cgagccgcca aagttgtggc agagcaccac
     8821 gcgtccgacc tcttaggcct gtaccaatca ggccatcaac caggcggtga ggccggcgat
     8881 cgtcgtgtgg gcgtggtgtg gcatacccaa ggatcgggga agagcctgac gatggccttc
     8941 tatgcggcgc gagtcatcct gcatccgacg atggctaacc caaccatcgt cgtactcacg
     9001 gatcgcaacg accttgacga tcaactcttc gggaccttcg cgcgatgccg agacctgctc
     9061 cggcaggacc cggttcaggc aaccgaccga gctgacttgc gggccaagct ggctgtggca
     9121 tccgggggcg tggtcttcac tacgattcag aaattctttc ccgaaggaaa aggcgaccgg
     9181 catcccgtgc tgtctgatcg tcgcaacatc gttgtcattg cggacgaagc ccaccggagt
     9241 cagtacgatt tcatcgatgg gtttgcccgg cacatgcgtg atgccctccc gaatgcctcg
     9301 tttattggat ttaccgggac accgatcgaa aagaccgatg ccaatacccg cgccgtgttc
     9361 ggcgactaca tcagcgtcta tgacattcag cgcgcggtga tcgataaggc aaccgtgccc
     9421 atctactacg agagccggct ggccaaactg gaattgaacg attccgagcg cccgaggatt
     9481 gatcccacgt tcgaagaggc aactgaggga gaagaagttg agcgcaagga gaagctcaaa
     9541 accaagtggg cacagctgga agctgtcgtg gggtcggaaa atcggctcaa gctgattgca
     9601 cgagacctcg tcgatcactt cgaagatcgc cttgccacaa tggatggcaa ggcgatggtg
     9661 gtatgtatga gtcgacggat ctgcgtggag ctgtatcggg aaattgttac cctgcggccg
     9721 cactggcatg ccgaggccga tgatcagggg tcaatgaagg tgattatgac cggctcggcc
     9781 tccgatccga tcgagtggca gggccatatc cggaacaaga aacgccgcga agatatggcg
     9841 ctacgctttc gtgctcccaa ggatccattc aggctcgtca tcgttcgcga catgtggctg
     9901 actgggtttg acgcgccgag tcttcacacg atgtatctcg ataaaccgat gcgcggccat
     9961 gggctgatgc agaccatcgc ccgtgtgaac cgcgtgttca aagataagcc tggtgggtta
    10021 gtcgtggatt acctcgggct ggccgacgaa ctcaagcagg cccttgcgac ctacacagaa
    10081 agtggtggga cggggaagac cgcgatcgat caggcgaaag ccgtggccgt gatgctggaa
    10141 aagtatgaaa tctgccgagg cctcttcggg tcatacacca cgcctgccgg catggtgaga
    10201 ggctttgact ggtcgccctg gatgaccggc aagccgcaag agcgactgtc ggtcttacct
    10261 gcagcccagg agcatatctt ggctcaggag gatgggaaag ctcgtctgct ccgtgcggtg
    10321 acagagctct cacaggcctt cgctttggcc gtgccgcacg aggagacctt ccggattcga
    10381 gatgacgtcg gcttctttca ggcggtgcgg gcggtactcg ctaagaatat tccagccgag
    10441 cacaaaaccg acgaagaatt ggatcatgcc atccgccaaa tcatctcaaa ggcggtggtc
    10501 tcggatgaaa tcgtcgatat cttcgccgcc gctggcctca gaaaaccaga cttgtcgatt
    10561 ctttccgacg agtttctcgc cgaagtgcgc gggatgccgc agaagaacct ggccgtagaa
    10621 ctcttgcgga agttactggc tggggaaatc aaggtacgat cccgcaagaa tgtggtgcag
    10681 gccaggtcct tcgcggagtt gctggaacaa gccgtgcgga agtatcagaa tcgcgcgata
    10741 gaggccgcgc aggtgatcga ggaaatgatc ggcttggcca aggacatgcg ccgagctcat
    10801 gaacggggtg aacgactggg gctgtctgaa gaagaactgg ccttctatga cgcgctggag
    10861 accaacgaca gcgcggtcca ggtcctcgga gatgagacct tacggactat tgctcgcgag
    10921 gtggcggatg ccgtccgcaa gaatgtgacc atcgactgga cggttcgcga aaacgtgcgc
    10981 gctcagctcc gtgtcattgt gaagcgcatc cttcgcaaat acggctaccc gcctgacatg
    11041 caggagaaag cgacccaaac cgtcttacag caggcagaag tgttgtgtgg agaagtggct
    11101 gcctaactaa gccctgttcg gttcggacca acgggcaggc tgtgagtcgt actcttcgcc
    11161 gcgaacgcaa agccccaccc agtcacagcc ccctttgagg gcaccccctc cttcaataac
    11221 gctccctctt tctgacctct cgtgtcgtgc ggcccgggcc atcatgtagc tctcccgttc
    11281 ttccttctaa agcacactcc gcaaaacccc gtggcgtcat tccgccgcct cgcgttcttc
    11341 cgccgtacgg cctcctttct cccccactcc aggcgttact gcagccagcg caagattaag
    11401 atgagccagc cgctgaagca tccttaagcg gtcgggctta aaaacattta ccgcgcactt
    11461 tccgaccgcg gcggcactac cttacgcaag gaggttcccc atggaatcgc ccccctacat
    11521 tcagctacct gcgctccaag ctctcggaat ggcaggccaa cgatctgctt gtacaagccg
    11581 acgtgggttg gaagcggttt caggcagtgg ccacaagcga aattctgcca gtccttcatg
    11641 aggccaaaga gaccttggaa agtgctggcc tggacgtctc gatcaccgac cttgatgaag
    11701 aaacccgctg cctcggcttc tatgtcaacg atgtgggcct cttctttacg ccagcagaag
    11761 atggactgac ggtctgtttc atggctcggc gcttcacggg aaaggaacaa ggctacgaaa
    11821 gcggttcctc tacaagcggg ccaaggcaga accgctccgt cgtctggttg aggaggcctt
    11881 actccgtctc ctcgggcccc gccgggccaa cgcagtaaat cagtaatggc agggtggtgg
    11941 cgcggccgcc ctcaggaccg tcgcgccact gcccagcgtt gcatcaacta acgacagaac
    12001 gacagacaaa gagcggagga tcaccatgac gaacgacagc gaagcattat tggggttaag
    12061 agtggcattg cgcgaaggtc aggacttcgg gctgttgccc tgtgaaaccg aggcaggtcg
    12121 tcgctttcag gtgatggtcg gggaactcgc cgcaccggtg ttgcgagagt tagttactct
    12181 gctatgcatg gaaggcctcg cgactcatct gcacatggct ctggacgagg caacaccgta
    12241 catcggcctc gagatcacgg agccatcgac cacgctctgg atcttcccct cagccacaac
    12301 caacgaaatc acgacgagcg tccagggagg cctctatccc gattacgcct ctgatcgcca
    12361 cgtatgctac cgcgcactca tgcccgaaac cttagaacgc gtactggttg aacagctccg
    12421 actggtgttg tgccccacgt ttcctattat ctaaccgtga tgaccatcgc cgaactgatc
    12481 acaaatttcg gggcactgat ctagcgacga tgttgtatcc agtcatgatc cacacacgcg
    12541 accgaatgac aggccaagct gaaggactcg cgtctcaact ggcctcagcg tggtggcaac
    12601 ctttcccaac atctctaact tcatcctgta tcggtccagg cccgtcctct acatccgtaa
    12661 gcccccggct catatctact tctccctcga tatcgcggct ttcgctcttt cattcgtaac
    12721 cgactgagat ctccgccgcc tctccggaaa aggaaactgc gagccatccg atcttgaacc
    12781 cttgagcacg gtcggcgcac gcgatgggtg ttcccatcca gccgcacctg ccttcaatcc
    12841 aactcaggag cccgtcatgt ctacatcaac acaccctccc taccagtacc tccgccaaaa
    12901 gatccgtgaa tggcaggcag gggatctcct ccagcccgtc gatcagcgcc taaggcagtt
    12961 tcgcaccatc acgccggtcg cccttctgcc acttgtcaga gacctgcagc aggcgcttga
    13021 agccgagggc ctgcgggcca ccgtccgaga gatgtcccta gaactgagtt ttctcagtct
    13081 cacggtcgat gacttcgaca ttgaggtctc gtttgggccc tgcgacgccc cggacgcctg
    13141 tcaaatgacc acctgccgga tggggagtcc ggaaccggcg gccattcggc tcctcgccta
    13201 tcgggatctg gagaccgatg tcgccggggt gatgggccag ctcgaagagg ccgtcctctg
    13261 tgccttggga ccgcgacgga ccaccactcg gaatccaatc ggcgagccac cagccgggcc
    13321 tcggtcatga gccagccgct gcggtaccca acagtcaaca tgctcgtgtt tcatcaccaa
    13381 ggagttcaca ccatgcaact atgtgaggct tacaccaccc tgcaaaacaa actgcagcag
    13441 tacgaagaaa cgggtcggct cacgccccgt cccgacgcca tgcgctcccg ttttctcgcg
    13501 ttcgcggaag ccgatctcat gcccctgacc cgccgcctgg ccaccatcct aacccaagcc
    13561 gggattccag cctataccag ggcgcaactg gaggaggaag ccatgtggtt cggcctgttc
    13621 ctgaatgaaa cctggtctgt aggcatctac gtccaaccgc ttgatgccgc cagtatgcaa
    13681 ttgtccctcc aattcaacgt ggactccgag gctgaagagc atcacatcct gctctaccgc
    13741 aaatgcaccc gagcgacgtt tgccacgacc ttggaacggg ccgtcgaacg gctcctgatg
    13801 cagacctggg aggagcgcag gcgttagctt tccttgaccc aatacatgca aaccacgact
    13861 cacgacaaag gagtacgacc atgccacatg caccccatac ggattatgcc cacctgaaag
    13921 cgcatttgga ggcgtggccc catcgccagc agacgcctga aagcgcttcg caagaagcct
    13981 tctcgcgcct tatagaagac cgtctacttc ccgaattaca gagactagcg ggggtcctgc
    14041 gggacgccgg catggactgt acggtgtttc gggaggagac cgagaccaca ggtgtggggc
    14101 ttcgtatcga agacctgcag gccacgatcg gactcttacg gggtgaacag gacacataca
    14161 tccgagcctg cgtcgcacgg gaccaacgac cgaatggcca gatcgagtgg tttattccct
    14221 acacgcgcat ttaccaaggc ctgctggagc gagagctcca ggcggccgtc gttcgtctac
    14281 tgaagtctcg tggtgcgggg tcagcgggga cgcaccgcgg gccaacacca taacgcggga
    14341 gggtacgagg agggcagctc gtgcacaaca gtcgcttccc cgtcctcctg actcaaccgt
    14401 cacacacaat aaaatcccat ggaggagaac gatcatgaac gacagtgatg ccttgtggaa
    14461 tgtgagactg gcactgcgcg atgatcagcc tttcggattc ttgccctatg aacaggagac
    14521 cgacttcgtc gaacaccttc aactcgccct ctattcattg aaatggttga ttccctatca
    14581 ccacatcgcc aacagtgggc ttgaaccaga gattctatcc gtcctgatga gctgactcct
    14641 caatgatcat ggagcaggaa tataaccatc cctacgaaca acgaaggaag tctgatgagc
    14701 agtacacaat ccaaaccctt ccgataccag gctatccagg ccacaccgac ttcatcctct
    14761 ccatctggat cgccaccacc agtcgatggc gcagccatcg accctattcc gtctgacatc
    14821 gacctcctac gcgcgatcca atgtgtgagc gtatggatta atcggcacct tcggcctgat
    14881 gaactcgttt tcgcgatgaa ggcaatcgag gccaaacttg acgagattat cgaacgcctc
    14941 cctcaagaca aaataaatgg ccaactcatg agcgtgaagg ccgccgcacg atttctgtcg
    15001 ttaagtgagc gcaccattcg agaacgtata gccatccgac agtggcccgc ctaccgaagc
    15061 ggcaacgccg tacgagtaga tccattcgaa ctaaaggctt tgatggagaa ggaatcgcga
    15121 tgaagtggcc acgcgatgct ggcaatccct ggcgcatctc gcgtgtggat gcccgaatgg
    15181 cagcgctatc agacactcat gttatcgaac tccgtgcaat cgcgctttgc ggcttcgatt
    15241 actggcagaa ggcgctgtca tcagctgacg caccgcatag agcgcctgca cagcctgatg
    15301 gctgcgagcc agcacttgat ccctaaagct ccgtacggca acgtcagcca ttccccgtgt
    15361 cgtaatcttg cgatagcttc gcgacgccca ttcaatgtcc aacgtcgaga gaagctcata
    15421 gcgttgcgga tccgacagaa ccccaactat ctgttgaata gacgtgtcgc gacgagggat
    15481 agcctcatac acgccacagg ggattcgggg gtcataatat ttacttctga tgatttcacg
    15541 aacatcgacg caacgcacca atagtttttt cactaactgt ttataaggag actcgacgga
    15601 caatctttgg cttacagact ttagtgtcca aagaacagcg tccgaataaa tgacaatcgc
    15661 gccctttgtt cctaatcgac ccacgagtga ttcaacaaac gccagtatcg gagatcgttc
    15721 ttgactgaga tctacccatt cctctcgatt cacagtccca ctcttggact cgcgaagcgt
    15781 ggtccattga aatgggactc gctcatacgg cctcagccca gggaatagag gcattccata
    15841 ccaggcggct tcaatgtgga gataatgaat gggatagcgt aatcgcttca tgaatgtttg
    15901 aagatgggga ctacgccact tctcctgtcg aacggcatgc cactggattg tggagagata
    15961 aaaaccatca ggaatttgat cgactgtctg gactccacgg gcaataagct tcggaatttg
    16021 ccgttctccc tctggcaaat accccaccca ctccgacgat ttcttgtgtg tacagtgtgc
    16081 ccagaactcg cacgggtatg gctgccaaca gtgggggccg ggctcgacct ttggaggatg
    16141 cggggccatc agcacagcct tcatcgtctt aagttgctgt ggaatctgct tgctcaccgc
    16201 ctgtatttta gacgacacat cggtcactcg taaaaaccgt tcaacgttga gtaccaagcc
    16261 tcgacggtat ccccgattga tgtgcatgac ggaataccgg cgcacgttca tgccgcaccc
    16321 ctgtaacaca tacgcttgca acgcgatatc ccagtgatga gcctccttcg ccctgctcgt
    16381 cgatttacat tcgatgaggt gccagctgtc atccgactcc cgttccagga tatcggcacg
    16441 gatgagaatg tcgtcgaaga ggaaagcggc ttcaaagatg gcagacaccg ttcggtcagc
    16501 cacaagcgcc tgcgtcttgc ttagggcgat gtttgggtca tgccgatgca acggcaccaa
    16561 tactcctcga gggaatcgct ggtgcgtaca ttcaccgaac acatgtccaa aatcaaacag
    16621 cgcttgttgc gcctctgtgg tgggtgttcg cagctctcga tgatgtacat cgagatacat
    16681 cctctttaag cactggcgcc cagccatgaa tttcgacttc gtcaggtggt acagacggga
    16741 aggactgatt ctgggcatct tgctattcgg cagaaggtgt gacaccgtac tcatgccgcc
    16801 aaaccagcgt cttctgacga ctcgggcagg attctcatca tcccaacggc aatcatcatc
    16861 tcaatgtcgt ctggcgaggt aggatacctt accggctgcc cgtgaagatc cgcgtccaat
    16921 tccaccctcg caagataagc ctctgcaata tcatgcatgg aaaaccccac cgatggagat
    16981 tcgaatagca cggattgctc tgccagatat cgctcgtact cgtcgagctc ataagtaggg
    17041 tcactccact cagccttggg cttttgggaa agaggagatt tggggtagcc ataaacatag
    17101 aggagtttcg cccgcagcgc gtcaaatccc aatcgtcgtg tggtccgatt gaggtgattg
    17161 acgatgccat tcacgcgttc cgcatagcca ttggtgacct tcacacagag accgttgaac
    17221 acttctgtgt cccatttctg gatctggttg attcgtggcc gaaacgcctg cgggaggacc
    17281 tgtagtgatt tctttttcca tgctcggtac aaggctcgag cctgtgtttt attcggcgca
    17341 tcccagatgt cgcaaaacgc ttctctgagc tcaaagacgt cggctagctg tggcaccgcg
    17401 acacgccaat aactgagcgc ctcgcgatca tgaggcgtga ggttgcgtcg ccgcttccgg
    17461 acaattcgcg tatcgtattt taccttcacc cgccgctttc gcttcacctc tcggcgaacc
    17521 tgatccaccg ccttcatccc aagctggaca aggtgaaact tatccacgac gatcctcgcg
    17581 tccggcaaca gttcccggac tatcttccga tagcgatcac acatatccat gcagacgact
    17641 tccacggctt cacgccccgg catggaccga agaaatgctc ggattgtctt ctcctgagta
    17701 tccggaagca tgtgatacgt cagccgcttc ttcagattgg tgaacacgac tcgcggaatc
    17761 ccatgcacct tcacttcgtc aatccccagc cactgtggag tgggtaactg cgccatcgaa
    17821 tctaacactc ggaaaaagga atgggcgatt ctccgaatgg ttcgctcatg catgccatac
    17881 tctttcgcca actggagaaa cggtcgatgc aaggatgcgt gggcaatggc cataactaat
    17941 cgccacgtcg cgcgccattc gggagacaac acctccaccg gctcaatgaa cagcagttta
    18001 cattgatcac atttgtatcg ctgccgagag agcttgacac gaaccggaga acctcgcagg
    18061 gggaggtcgt cgaagctgat ctccttttgg ccatacttca ggagcgacat gctgttctga
    18121 caacctgggc agttcgctgg cggtgcctcc gtgacggctc gaaatttcca tcgctctccc
    18181 caagacacag accatacttt gatcccatcg agatctagca ggtgtgtgta gcgggtgtgg
    18241 ttgtgggtga aagactgcag ccgatcaaga tcaaatcgtt ttgctttctt atcttgagga
    18301 gacatcttcc caactgtagc aggttcactg aaagacgccg actgtgacga ttgagaccac
    18361 tgacctcggg catccgttag tcgtcgttct ggttcgtttc tgcggccatc ccactttctg
    18421 ccgaaattcc tgtacctgta tacctaagcg cctcatgtaa gtcccttttc tcagtcgtcg
    18481 acgtcttcgc ctttcctccc agtcctcgtt cacgggtctc ctccttcgta tccgacatca
    18541 cgacttctcc ctctactccg acgacctctc ccggcacacg actttgaaga cgggccaacc
    18601 caggggactg gtgccgtgtt tcactttcac cctggaggtt gttccgatgg tttctcgcta
    18661 tacccgtgtc cgcaaaggcc ccttcacctg tgcgacgtac caagacggta cgcaatggat
    18721 ctatgtgcgt gtgtaccacg cgcatgatga gtacacgcta ggccgctacc ccacacaaga
    18781 tgacgcgcag cttgcttatg atgagttttc tttgcagcag gcgcttcaac ggtttcctga
    18841 actgacccca gaacaacagc agcttctgca acagacgata actgcccacc ctcgcgtcgc
    18901 gaagccggtc gctcggatgg tacggaattg gcgtcccatt gagctaggcc cggacttccg
    18961 tcaccgcaag ccctcctcgc caaggcgtca tgcatcggcg gagaaaccga ttcgtggcat
    19021 acgacaggtt ttcttgaaga atggagaacc tcgatggatt ctggcgctta cccataacca
    19081 tgacgtgaag tgccgccgct tctcatcgtt acaggacgcc gaagactact accgcgaagt
    19141 ccaagtgcaa cgagcgttag cagcctttcc tccattgacc gatgaccaag tgaaggaact
    19201 cacggcgctc cagaaagagc cgttgctcag gcccgttgtg gacctcctca ttaattgttg
    19261 gatgcttgcg ccgagactga agccaaagct caatcgacgt cagggagcct tgcctttcca
    19321 atcaggacgg cctcaacacc agagccaaaa gcctacgggg ccaccaccag atcctggcat
    19381 tacctttgcc gactatgcct atcgctggtt cctcgccaca gctgaccgct ggtcccctgc
    19441 ttctcaggac atcgttcgat cgctctgcac ggcgcatttg gtgcctgcat tcgggccaac
    19501 tcctgtgcgg gacttacgca ccgagatgat cgcagagtgg cttcagggcc aactgaaaca
    19561 gtattcactg cagcactgct gccacatgcg gactcagctc gtgagcattc tctatgccgc
    19621 agtggcggat gggctgatcg ccagcaaccc agccgtgggg ccaggctgca tgctgccctt
    19681 caaggccctt cacaatcaac gtgcacggta acaaagaaag gacacacaac atgacgacga
    19741 cgaaacaccc taaggactcc aaagatcgag gcatctgtga gcgaaaactc agcaacggca
    19801 cgattattta ctacatccgc ctctaccaca atggaaagcc cgtcgtcttt gggcgattcc
    19861 acagcaaggc tgccgcacga aaggtctacg cgaaggcaaa agtggagcaa gacgaggggc
    19921 ggtttgatcc gaccaaatat caagtccggg ggccggctcc caagaagctc cagggtccgc
    19981 tgatctttaa ggactatgcg gaacaatggg tcaaagaaca cagagctacc tataagcctt
    20041 cgacgttgag agggtatcgc tcgattctaa gaagccggtt ctatccgacc attggaaacg
    20101 tccctatggc agctctcaat cgccaaatga tcaaggacat catcctggat gacatggatc
    20161 ggcgcacggc cgatggagag gagctcaagg atcggaccaa gcggggagtc tatcaggtca
    20221 tcaaacgggt gctgaaatcg gcgttccttg aggaattgat ttcacgaaac catgcagacg
    20281 atctccatga gttccttcgg tctccggcca agtttcttgt caccccactc acggaggaag
    20341 aggagaaaca gttcctcgga gccgttcagc gctatgcccc acagtggtat gtcttcttct
    20401 tcctcctact gaggacgggc ctccgcattg gagaagcctt ggccctccaa cctgccgaca
    20461 tacatttcga gaaacgctgt atctctatcg ttcggaattg gacctttggt ggcattcaaa
    20521 ccactaaatc ggacgagggc cgctgcgtgg atctcgcgcg aaagcttgcc gaagtgatcg
    20581 agaaccatat taccgagcaa cgactcgaag ccgcccgtca ttggacacaa cccccgatct
    20641 ggctgtttcc tggaaaaggg gaacacccca tcagccagga ccacgttcga accaacgtct
    20701 tccgcatggt gcttcggaaa gccggtcttc gccggtttcg gattcatgac ctccggcaca
    20761 cctttgctac ccgtttgctg ctgaaaggcg accgcaatgg gatcactctg aaatacgtga
    20821 gtgaacaact cggtcatacc tcaatcgcca caacggctga catctataca cacttacttc
    20881 cgcagggaaa cattggggcc attgatgccc tcgatgaact cgatgaccgc cccaacgaag
    20941 agggttcgcc gggcaccgct actccagcag acactgaaga agcggcctag taggaacgcc
    21001 gtgccaacac agctgtttgg cgcggcgccc ttaacattac tggtcccccg ggagtgggca
    21061 agagcttgtc ctttccttac accgggctcc acgccatgca acaaaatcgc ggctgttcgt
    21121 ttcgcgccca aaagacgcaa tgagccaaca tgcatcgaat tgaatggtgt accgacactc
    21181 aagtgacggt gagtgtcgga aaaacctgac gtcacatatg gagagtttta tgcgaaaaga
    21241 agcatcccac gggatttcca cccgtcaaca cgccaatggc cgaatcgaat tcacggcctt
    21301 cctctaccac gccggccgac ggtgggtggt cggtggcttt tccacgaaac aggacgccct
    21361 tgccggaggc cgccaattaa gacggtctct cctctcttac cactcgagtc gcgaccacga
    21421 tgcttcgtaa gtccagcggt tcgggactgt ccaattccac gcagcacact cctcctggag
    21481 gaacagcgtg ctgcggactt ccgatcgcga aacccatgtc ggtttgggcc aacggaaaca
    21541 gccaaaacca tccacgatct ggaccgtgcc tatcgggagc caccttgtgt cggacagttg
    21601 aatacgtcca cctggaaccc tgaacatcag ctgtcccgtc gcgacggcac cgaccgccag
    21661 cagccgctga tgtgagaaca ccccgccaaa tcgggacgcg gatctcgcca gcctcccacc
    21721 attcccaagg gggcctctac ggaggaaagc acgccatgtg cgctcgccgt cgcgacggcg
    21781 agcggtacac ctctagacga acatagtagg ggaagctttc tggaccaatg gccggagagt
    21841 cgagcccgct ttgtctgcat gctcgcgcat cacagccaaa gacctacctg gaaaccctgc
    21901 cggatccagg gcccatgtga ccgtgcgtgg gcgcccttac agctcttccg ccacctagat
    21961 tgttagtagc agagcggatt gtcccacttt tcagaaaggc gaaggccgtg gccatcaaca
    22021 catcccaccc agatcacagc cagcagtctg ccacacgacg tcgagtcagc gcgaaatcac
    22081 ccgtccctat ggagacagca accctcgcct cgcctccaca cgatcgcgcg gccagtgctg
    22141 tgacgggagg ccccgcgtcg tctgatgcca ccatgaacag cgcgcgtacg cacagcccca
    22201 ccccgctcgt ccaagcactc ctgaccgccg gaggggcagg tccattgccc gaactgctca
    22261 cgctgtcaga agtctgtgcg ttcttgaagg tgaagccgag ctacatctac tcactgacct
    22321 ccaccgaccg cattccgttc cgcaaattcg gtggcacgct ccgttttgtg aaagccgagc
    22381 tgctccagtg gctcgaccgt gggcgccggg gcccaagata agcgggccat ccctgcatgc
    22441 ctcagaacga gagcggctct tcactcttca tcaacaaagg tttgggtcat ggccacgacg
    22501 cgtactctgg aaaaaggctg tctgtatcgg agaaagaatc gaccgacgta ctacgtgaag
    22561 ttttcgcctc ggaaaggggc taagccgatc cacctcagca cgggcaagac aaatttgctg
    22621 gcggccgccg cgtcggcgca ggtgttgatc gcaaaatatg acggcaagtc gacaaaccag
    22681 gtcaaaggcc agaagctgac gatcgcagac ttctgccggc ttcccgacaa gcacgatccc
    22741 accgatcgcg gcgggcaatt ttggcaatat ctcaccgcca atcgtgctgg gaacacgaga
    22801 acacggtatc gcgatattct aaaaacccag atcatcccca ccttcgggcg tctgcgcttc
    22861 aatgaggttg agcctgaagg aatcgaagcc tggaaacaac gtcgcctcct tcaggttcag
    22921 cgctcgaccg tcctcaagga actccattgc ctctccgcga tctttcgcgt gtctcgaaag
    22981 gtgtatcgct atacgacgca gaatcccgtg ggcgacatcg aaaagccgac cgtgcccaag
    23041 cggaaaaaac agattcccac ggccgacgag atcaaggcgt tcttggacgc cgcaggtctg
    23101 ctcacgcccc actactactc gtctttcttg accatttatc aaggcggact ccgtatcgat
    23161 gaggcgcggc acctggaacc gtccgatgtg gatgaagaag aaaacgtctt acacattcga
    23221 gtgaagacgg ggtggagccc caaggaccaa gaggatcggg atattccact ggtcgagccg
    23281 atgcggaccg tcctcctgac cttgaaacac cagcacccgc acgcccgctg gctcctcccc
    23341 cgcggcaaca cgcggaccta tacgtgtcag cgctgcggtg gaccgaccac ccatattggc
    23401 aatctccgga cggcgatcct ctcgctcgcc acggccgccg ggatctccaa acgcatgacg
    23461 catcacatcc tgcggcactg caactccacg cacaaccggc aattgggcgc gaaagactat
    23521 gaggtcatgg cattgctcgg ccagcagtcc acgaagatcc ataccatcta cacccatgcc
    23581 gaatggcgga acattgtcga tgcgacggcc cggctgggcg tgtcgatcgg gcaggatggg
    23641 ttgtccgcgt ggttgtccgc ccgggcgcgg gaggctgagg agtccaatcg gtcaaggact
    23701 taggacgtcg ccatcagccg ggcaagagct gtaagcagca gggccatttt gtgccgcacc
    23761 cgacaagtcg gggagacctc tggtgtgatt tttaggcaat cgtaaggcga gtcgtatatg
    23821 cgtttggatg gccgagcatc ctgcaccttc acaatatcgg catctgtgac agagtcttca
    23881 tggcagccgt agtctccacc gaatgcacag gcagactgtt gacctcgtta ctccggccgg
    23941 gttaagtcaa tgatcagcaa agaaggtgaa atgcctacgc tgccacgcaa ctcaatcgga
    24001 ttcttcgcag gtaatggaat tggctaatcc agcgcgatgg agtctcgttg gtttcacaaa
    24061 ttaggagatt gaacaagtat ggtaatggtc agcttcctcc actctcatag cttggttatg
    24121 tttttctatt gtgtgagtct gctcctcgtt ggatgcagca caagtagccg gacattgacc
    24181 gctttcgatc tcgtggtgaa aacaccctcc atttcctggg cagagaaaca gatcgtgcga
    24241 cagcgagggg tctaccttta ccgactgatt cctgagaatg aggttgatcc aaaacgacat
    24301 cgcgctgcat tcctccaacg gaaaaatgag ctcttaggca ttgcggttga acaatttcct
    24361 atcgcctctg gcagctatgc aaacggcgag atacgtttcg tgcttccaga aaagttcgag
    24421 ccgagcaccc attgttttgc cgtggttgca gcaagtgggt atttgatcgc cacttctgcc
    24481 tttggatccg acaataagcg atacttgata ggcttgtctc cctatagcaa acgatatctt
    24541 ggctctcccg gagacgcttc cataagcagg ggaggaaata tgttttctac aacgaccgac
    24601 cttgaatgtg caggaacatt ggaagatttc acaagtaaga attcgcagct atattccgtc
    24661 ggtacgccct agtaatgtga aggtcctttc gcagaagtaa ctgtttatcg gcgtctcgca
    24721 acaatgcgtt cgtctactct gaggggggct gcacagtgtt ttgacgacgg gcggcgccta
    24781 caggcaggac ctccacgtgg catatcctta aacatcgcag gccctctgag cagagatatc
    24841 gcattcgcga ggtatacatg tactgcatag agatgcattt aactggctaa gagtgccccc
    24901 cgattacgta aaatcaaata tatcaaaggc ctatggaaag caggggtaag ggtgaggaaa
    24961 cccttttctt gtgcaaaagg gttgtggcaa cgtgcgtggt ttataaacgt ccggaagcgt
    25021 gcccgctcca ttgtaagcgc cgatagccca aaatcccaga aatgtccaaa aggcagcttc
    25081 aaaagcctgg tttcggagtt gagcatcggc ctgctgcagc ttcccaagcg catcgacgta
    25141 gagcttgttg ttgggttcgg ttcgaaccgc ggcactgcag agaatgatcg cctgctgcat
    25201 gtaatttctg gttccagcgc tagcataaag cagtgcacgt aaatagttct cctgatcttg
    25261 tttgaccccg ctactgagaa ggtaccgatc agcctcccta aaatgactcc actctttagg
    25321 atccgatcct ctaaataatt cggaatataa caagcccaat tggagatgag ctccttccac
    25381 taagggatcg agttgaatgg catttttaaa atctgctaac gcttgggaat tttgttgaag
    25441 agctcgatga cacagacctc tcacgaaata ggcctcggca tgttttggat ttcgactgag
    25501 cgtttcgtgg acgtctcgaa gagattcatc aaacctaccc agagttgcta atgcttctgc
    25561 acgacggtag taaagttctg cgtcgtttgc accgaccttc aaaagatctg tgtaaaatgc
    25621 tatgagcttt gcgccgtata aagtcgaatt caatttcaac gcactgctcc aatctgtaaa
    25681 tgcgcgagag cgatcttcac gcataaaatg aagcgccgcc cgaagtgcaa atgcactatc
    25741 attctctttg ttcagctgaa ctgcttggtt taagtcgagg atagcctcgt cgaagcggtt
    25801 caagccagca aaagcatgcg cacgttccat atacaagtcc gaggttatgt gcttcatctc
    25861 taacgccttt gcatattttg ccactgcttc ctcgtaccgt tgtgctcgaa ccaaaacaga
    25921 tccctgaatg atgtaccctt cagcaaactg cggggtgaat gccattacct gctcgatgtc
    25981 tggtgctgcc ttcgccatct cctctgaagc gaggagatgt atggcccgcg ctacgcgtaa
    26041 tcggaatgac ctcggagcga gactaatgcc tttgtccaac agatctgaac ttttggggca
    26101 gtgagaggtc tcacacagtg ataaatagag cccgagatat gctccggcag gctcgaagcc
    26161 ctggctaagg aggatgctga acttttcacc agcaagagga aatcccttcg atgcataaag
    26221 ctcccacgcc tctgcgtaca ttctggctgc ttcccattgc tctataccaa actcccgtcg
    26281 agcgcgggta taaggcccca catgatctgg gtaattcaga tcaatgacct tctcaaagtc
    26341 gtcgagtgcc gtacgttggt gccccaggat agcttgatac cctacccgat catcatagtg
    26401 ccagagggtc attggatcgc ggacaacgct gagtttattc tgcgtcgctc gtagaatatc
    26461 tctacagtcc tcgggtttct cgcaacgtcg atgcgagatc atttcatcta ctcgatggaa
    26521 cgattctaaa aatccagtgc agcctagtgc gcttaacagt ggaacaaacg agatcacctt
    26581 gaggagaggg cgaaggtagg caccagtcat aactcaactc cttgggttcg cattgaccga
    26641 gaagctaaat gaggttcgag aacagaacct ctatcccatg ccgagtaacg gtaattttga
    26701 ccgacggatt agcacacaat cacttgttac aggccactag tgtacgatcg agaacaagct
    26761 gacgctctgt cactagcaaa aacattgcgt aatttgcatg aactattcga tggacattcg
    26821 ccagatgtct ccttgcttaa gaaagcgtcg tgtcaaggtt tctccgatcc cttcttgacc
    26881 tcccatcatc gcctgcagga gttggttgat tgcagccgca tgccgatcgc ccgcgctagt
    26941 gccctcgttg gaatgagcgc tcacttttaa accagcgcgt atcttccacc ttgatctcgg
    27001 cgagcctgcc ttcgacaacc tgcacggtga ggatgccatc cgtcacggtc tgctcaggga
    27061 taagggcgcc cgacgtcaca tatccgtgat tgacatagta ataggtgaga gcgagccgaa
    27121 gggcctcgag ctcttctgct gtcacgtcgc gattggtata gggtccggtg acctccgtca
    27181 gttgttgcga ggtgaatgtc gtgttcccga tcaggcgcac ttccttgacg aagatcttgg
    27241 cgccggtaat cggctcacca gggacaggcg gcgccggtgg gggcacttca agcgccggcg
    27301 gctgagccgg cggcggttcg accggcaact ggggtggttt gggcggcagc ggcaccccaa
    27361 cctgcgcaag tagaggtgtc gcacacagcg ctaagtggaa cccgagcaga attcctaagc
    27421 cccatgccat cgggacaggc cccatcgatt tctgctgata ggccatctcc gtaacttgcc
    27481 ctccccggct cagaatagaa ttgacatacc tgcagaacac atgccgtcga atcgcatgga
    27541 aatggttcca atatgcaggt gccgaattac ttgccaggaa tgtgcagagt cagccaaact
    27601 ctacgagtgt gatactgacg accatcttcc cgttgaatca ctttcgatac agtgtacgta
    27661 tctcttttga tactgaccgt gacgggatcg gaggcatggt gtgaggagaa gacgacgtga
    27721 cacttagatc aggccagcac gacggaagct accgaaacag agtggtatgg ttgagcactt
    27781 gagggtcgga gtcaattgcg ggtggaatac taccctcagc tatcgatgac ccgacacaat
    27841 gcctgaggat tacttcacga cattagggac tcaggcccgg gcgacgatcc tttcggtacc
    27901 cctcaactgt tcgactgacc gtggttaagg agagcgcttg ttccaccccg ctcaccttaa
    27961 gaccacgcag cccttgttgg aggccctgca catacgcgcg tccatcatca cactcggtgg
    28021 atttaccctg gagggaagga tcgaagggtt tcacgccgac aatggcctta atggtctccg
    28081 ccccaaaagg acccgtgacg gtgaattgct ccggagacgt cgcactgccg aacgagtagg
    28141 tctgcccgcc cttaatgaag gcctctcctc gataaagatt gggaaccaga tggaccacac
    28201 agccgtcggc ctgaaaatag tccagcttca gatagccgtc gcggtcggac tgcacgtgga
    28261 tgatcaactc ctccccttca ctgaaacgac catcctcccg attggtccag acgcgtaacc
    28321 cgaacccagc ggatgccgca accatgggag cttgtgcttg ctgcgagatc tcttttgcgt
    28381 taattcgttg tttgatgagt tcgtcgatcg acacgggact catccgcgcc gcaatcgtga
    28441 tacagatctc ttggccatgc ttctcctctt tctccacttg aacctgctcg agcatcgctg
    28501 cactggccgt ctgtacgata tcttcttcca gctgaagatt cttcactttt gaggaggact
    28561 gaacaaaaac tttgtgaaga cgaacagcgt cttcttgtgc caaagcgatg gcaacacgtt
    28621 ttgcctgaac aggggtttca ttgtccccat acttatagca agcagaccct cgaattgatt
    28681 ctgttggccc ggtttctccc ggtggctgac tcgcgcgggc aaaccccatc cagaacaccg
    28741 gcatcaacag gactacacat ccagcactga tccaccgctt cagtcgcaca ccgctactca
    28801 taatgcctcg ctacaggcta tcgctttgcc cctacaatat cctatttgcg tcaccggact
    28861 cttcatctcg cttctcacca ttattgggaa ccgctccgat cgacaaccac aatcacctgt
    28921 cccttctcca ggaattcaaa atgatctttc actccgtgaa tcgtctgggc ggcggcattg
    28981 ctgatgacaa gatccgtatg gctgtttcct gccggtcgaa gcgctttcac atacagcgga
    29041 ttgtccgtga ccttcggatg agtttttgcc gccagagggt cggacacata ggcggcgatt
    29101 ccttgattct ggcgatactt ggcgagcacg tagctctggg aataggcctc ttgcccgtcc
    29161 tcggtgagaa ttcttggcac taaagccggt cgcacctcca tcccgcgcgc gtccaccaca
    29221 agtcccgtat agggggcggc cgttttgggc cgagcatctg gcgggagttc ccgcacaaga
    29281 cgctttggtg cgggagcatc ttttggcgca aacatctgcg atgtttgtcc tgtcaatttc
    29341 atttgcagcg tgacttcata tccacccttc tctaattccc gctcctgaat gaccttcgca
    29401 tcctcaatca tcccctgaac cttcacctga atctcgtcac tggtagcgac gtaatttttg
    29461 accgtcgtgg tcgaatccac gcggatcgcc gtgaatgcct ccagcaggtt tcgataggcg
    29521 acggcctggg ccgcgcgtct tgccagtaac cgagcctgcg tgctgttgat tgtgtccgaa
    29581 ggcggatagc cgatccctaa ggcggtcacc atacccgccg tccagtccac cattccttcc
    29641 tgtcctagct tttccgtata agcagttgag cctgcgaaac caccatcact accagatgcg
    29701 ggtgaagttg aaaccggcgc gggtgaagtc tggaccggtg gtgaggctga agatgtcggg
    29761 cctgctgcat gtgctgagct aaccgcggga gtgtttgaac cttctttgcc ccgcacctcc
    29821 ctatccccct gacagcccat gagcaatacc gcgatgatac cgaccaacca gtgtttcgtg
    29881 tgatgcatca tccgcctccc ttcggctctc attcgaggtc cgtcctacct acccacatgc
    29941 aagaaccatc aataatcatg ccttgcccat cgctcttccc cacggcatca acgttctcct
    30001 cattcagtcg gctccatgtc gaagccgtac cacggatttt tcaagctcta tccgaatacg
    30061 ggccttgtga tttccacctt tcggacaggt cgtcttgttc tctcgcgaga ctttcgtata
    30121 ccctattacg caattgcacc tccccggcac cgcccgatga gaggccccga tgccagaccg
    30181 gatcttttcc gggaaatacg ccgtccagga agaaatcgcc aagggcggca tgggggtgct
    30241 ttataaggcc ttagaccgga ctctcaatcg cctcgtcgca atcaagctca tccacgaaca
    30301 gttctccggc gatccctcct tcaccgagcg gttcatccgc gaagcccgcg ccatggctcg
    30361 gctccatcac gagaacatcg tcacgatctt ttccgttgaa gaagatcggg gcgctcactt
    30421 tattgtgatg gagtattttt ccggtatgga ccttcgggcg aaaatcaagg cctctcactc
    30481 gctttccgtc cgtgaaagcg tggacatcgc gcttcaggtc gccattgcgc tggcctttgc
    30541 gcattcccaa ggcatcgttc accgtgacat caaaccagcc aacattctgg ttgatagcag
    30601 agggcgtgtc aaattgaccg actttggcat agcggccgcg ctggatgaag cgtccattac
    30661 cagcacgggg caagtcatag gaacgcccga gtatatgtct ccggaacagg cgggaggcat
    30721 ggcggttgac ggccgcaccg acctttattc gttgggcatc gtgttgcatg aaatgatcgt
    30781 cggccaaact ccgttccgca gcatgccaaa gaccagcatc cttaccaagc tgctcgatcc
    30841 agagtatgaa atcccactga tattttcttc cgagatacca tcggcggtga aagcgatcgt
    30901 cgaagacctg gtccgccgga atccagagta tcggacgccg agagccgcga ttctcgcaag
    30961 ccagctgaag gaatgtctgg catccctccc cgtgcaggat agtgacgatg cacccacgat
    31021 cgtcgccacg tcgccatcgc ctggtacgcc acgtacaacg cgggtcggcg ccacgcctcc
    31081 acctttggaa tcgcctacta tcccatcccg gcagaacgca gggccacaga tcgagtcagg
    31141 gccacaatca ccggccggac aacctcaact ccctcctatc tcctccgagg cgcgtgatga
    31201 agccacagat gttcttccgc ggacaaaacc ggcctcgcag ccacccccgt cagcgccaga
    31261 gagcaaacct attcggcgaa cgtacaagcc ggtaccgatc gcgatcggag tcctgagcct
    31321 gatcctggtc ctcagcggcc ttgtctatgt cttatccaat ggcatccggc tcacgactcc
    31381 ttcggcagac aagaccgaac ctcctgcctc ctccaccacc actaccaccg aaccagccaa
    31441 cggcgcaggt gcgcctcacg acccggcctc cagggatagt gcccaactgc aatccaaaat
    31501 agaacaagag cagcgacaac ttgagataga tcaggccaga gtcgaagcga aaagccgcga
    31561 agcagaagcg cttcaacaaa aggagaagga ccaggcagca caacaggcga aactcgccga
    31621 ggagcaacgc aaaaaaaccg aggaggcggc tcgtgtcacc cgtgaacgac aggaagcaga
    31681 acagcgcaag tttgcccaag agcagaagcg tctcgaaaaa gagaaggagc tcgctgccaa
    31741 gcaagctcag gcggcggaag aacaacgtcg gaaggcgcag gaggaagcgc gtgtcgctcg
    31801 cgagcaggag gaactcgaag ccaaacgggg caaagaggag gccgagacga agcgtttgca
    31861 gggtgaacgg cagcgacttg agaaggaacg ggacgcagct gaagaacagg ctcgcctggc
    31921 ggaagctcag cgcaagaaga cggaagagga gacccggctg gccagagagc ggcatgaagc
    31981 ggagcaacag cgattccgtg ccgaacagca gcgtatggaa aaggaacgcg aactcgccgc
    32041 ccagagagca ctcatacaag aggaggaacg tcgcaaagcg gaggaagcgg cccggctggc
    32101 gaaggaacgg ctagaggcgg aacagaaacg gctacaagct gaaagcgatc gcctggaaag
    32161 agagagagag ctggccgcac agaagattcg tgcagagacg gaggaacgcc gaaaagccca
    32221 ggaagaagcg cgtgtcgctc gcgagcggga tgaagcagaa caaaaacagc tccaggcgga
    32281 ccgtgaacgg ctggacaagg aaaggcgcga ggccgcatcc ctacaggcca agacggaaga
    32341 ggaggaacga aaaaagcggg ctgcacagga ggaagagcgc agaaagaaag aggaactgag
    32401 cgccaagctt cctcccgacg gtctgttggg tgccaccgct tcacggcggc aattgcgtga
    32461 cctccttgat gaatttaagc gagcctatga agggcaagac ctcgaagcgt tggagcgact
    32521 ctccgagatg ggaaacgacc gactgggttt cgtgcgcacg atgttcaaga actatcggac
    32581 gattcaggtg tcgattcaaa gtctcaccgt gatagacgat gaagccgcgg cgaccatcat
    32641 tcacgaggtg ctgattaaca acaacggcga aacggtcaag ccgagcccga ttctcagatc
    32701 ggcgcgaatc aagatccgta agaccgggga tagatgggca aagattgaat ggtagcgcgt
    32761 cacggacggg accgtacctg ctcggcgaac ggattctgaa aatcgaaacc gaacagcgcc
    32821 ggattttcag cgatcaccgc ggcggaaaaa atgtagaaca cataatcata ggtctcctga
    32881 ggaaccttgt gctgcttcaa taactgccaa aaattccgct ctctcggatt gttcggcatc
    32941 tttcggataa tgttgatcac attcccctcg ccccaattat aggacgccat cacgagcagt
    33001 cccgatgcct gggcatcggt gttatagatg cgcctgatat atttcgccgc tgccttcgtc
    33061 gctttgtgaa aatgaaagcg ctcgtcttgc ggatcgtatt gggcaacatt tttcaatggt
    33121 cctggcttca acccatattg ttcagcggtt tgtggaataa actgccaaat tcctttcgca
    33181 tagcccattc gagtgggagg ccccacggct tttgagtcga agccactttc ttggagcgcc
    33241 aaatagaaaa attggggcgg caggttttgg gtccacattt cattggccac cgtcgggatg
    33301 aacccgttct ccctggcgcg cttaatggcg ttgtgcagcc gttccgtcga tttccaacgg
    33361 cggatatatt tcttcacctc agaaagaaaa tcccgcggca tggccacctc gcattcgccg
    33421 aatagccgcg ccatccgaaa cacgagacga tcctgctcac tcatcccctc ataaatatcc
    33481 agttcgccta gaaagcgatc atattgcacc tccatttccc gaagtcgttg gcgcttactc
    33541 agaattttct ccatgaggtc tttacgctta gtcgcgacga cttcctcttc gagttccgag
    33601 acctgaattt cgacctgctt catcgtgtag aacatctctc cagcggttct cgtcatctga
    33661 tcgacccttg cccgttggta caaagcatat cccaaggcgc agaccaaaag gacgacgaca
    33721 gccgcgactg ccccgcgata ccagcgtttc tgttgtcgct cccggcgctt ggccgcctcc
    33781 cggtaccgac gagcctcctc actctcttcc acggtgtctc cccgcttcca gcggtcgaca
    33841 atctcggtca ccgactccat tttcttgccg gactgagccg catcctttga ctgatcgacg
    33901 aaaggagcct cgtcgcccac cgcgagccat agggccggtc cacccaatcc cagttccacg
    33961 gtcactggat ttttcagctg aacccgggtg acctgacgtc cgtcgacata caagccatgc
    34021 gtgctgtgat gatcgacaag ccaccattgg ccggcctcca acctcacctc cgcgtggata
    34081 cggctgacca ctcgttccgg taccaccact tggcaatcgg ccgatcgtcc gatcagaaac
    34141 gtgtgaacaa agcgctgctc gccgctccac gaggtgccta tgcagacccg tacgcgaacc
    34201 gactgaggtg tgttgagcgg ggcagttaag tcttcagaca tgtcgggaag gtaacgggcc
    34261 tgtgatcgac tggcatcgat tcgattgagt agacttcagc agcctgagcc aacagatcga
    34321 cctaggcaag cgcttgctga agttgagtgc gtaagggatc ttcgaagcgc tcgatcgact
    34381 caagcgaaac cagctctacg aaagcaggaa cgccggaaaa gcgcgtcgcc atcagcagca
    34441 catcatccgc gggacggaat gaagcgagag gagcggtgat cgtgacacaa gacaaggccc
    34501 ctaaacccgg caagggtttc atgtcgatct ggtagcgcaa cgctgccaag agctggacca
    34561 gattgggttg gctcttcatg agaagctgca tcaccaatgc attgacgacg aattgccgca
    34621 aggcctcggt cttccgctcc atccggctct ccatcatcga atgcacttgg gacagtgtca
    34681 gtccggactc gtaactcagc acccattgca cgggggaagt aatcgtcgca tccttgaagt
    34741 cacgttctga cttggctctg tgagtatact cccaccggta gatggccggc tggctatcga
    34801 gttgggcgag ggcggtatcg ggaaattcag gaggcagccc gaacggactg tggctcccct
    34861 cgcgatacag ttcctttaaa cgagtgagag caagatctga accgttgacg tcatcgcgtg
    34921 atccgacatg tcggcctaac aaccgtcgag gagtcaacaa aggtttaagg ggttctatat
    34981 aaacgcgtaa ccggctgtcc aagatgtggg acaccgcatc agtctttcca cgaagcgagg
    35041 ctacctggtc catggtgagt gcctctcccg ttcgcgaact ctccatctgg tacacctccc
    35101 aagtgcggcg ccattatcga gtgcggcctg ctgaaaatca agattagcct cacgaacact
    35161 gatacgcgag gctcgcattc cagtcaggct tgctcgtctg tttgtcgccc tcctgtcacg
    35221 agccggcagg gcgctccttg ttcttctcga agcgtttcag ttaggctaga atgaccctgc
    35281 tccctgcgcg cctctccctg gagatgttga atgaccgacc aagtcttctc cggcaagtac
    35341 atcgtccagg aagtacttgc gaagggcggg atgggtgtca tttacaaggc cttggatcgc
    35401 tccctcaatc gaatagtcgc catcaaagtc gtccacgaac atctgtccgg cgatccctcc
    35461 tttaccgagc gtttcctgcg cgaggcgcgc gccatggcac ggctggacca tgagaatatc
    35521 gtgaccatcc atgcagtcga ggaagaacgg ggcactccgt atatcgtcat ggaatatctt
    35581 tccggcagca atctccgtgt cttcatgcaa gcccgcacac cggttcctat tcgaacgagt
    35641 gtcgcaatcg cgctccaggt ggctgaagca ttgtcctacg cccacgatca agggatcgtg
    35701 caccgcgaca tcaagccggc caatattctg gtcgacagcc acggccgggt caaactgacg
    35761 gatttcggaa tcgcggccgc cctcgacgaa gtggctatta caacggtcgg tcaggtgctt
    35821 ggcaccccag aatacatgtc tcccgaacaa gccagtgggg acaaggtcga cggtcgcgcg
    35881 gatctgtatt ccctcggcat cgtgctgtat gagatggtcg tcggacatca tccattcggg
    35941 aatctgccga agacggtgat ccagagccag ttgctcgata caacccagga aatcaggctt
    36001 gcctttcccg acactatccc gtctctggtg aaagccatca tagaggacct cgtgcgacga
    36061 gaccgggact atcgtacccc gacggcccga ttgcttgtga gccagctcca ggaatgcctc
    36121 caaacacttc cacccccacc cgaaccaacc gaagaagaac gcaccatttt cgtgacgcct
    36181 catcccgaga ggcaaactga acagcgaatg ccgccgcccg tctcttcagg aagcccctat
    36241 cggccttcta cgccccttcc tccaccccca ttagggacac caaaaccttc gaagggcgcg
    36301 gaccatgtag tttctgttcc gtctggtccg gaggataaaa cggacgtgca tatgcagcat
    36361 ccggcgcaaa ggaagccgga atctcctcat gtgccgcaac ccttctggca aagatataac
    36421 gtccttccgc tcgtcgccat agcatcggta ggagtcctgg cattaggagg catcctcctc
    36481 tacgtcaatg tcgttgacca accacaacca ccggagctcc ctaagaaaga agagcccctg
    36541 gttaatcccc caggaccgcc tgctgacagg gaaaccggag accaggacag agtacctata
    36601 gaggagacct cagagccgga cagaaaagtt gtgccgtcac caccgccaca acccacaagc
    36661 ccgaaaggca ccagtcggcc gtcaacccgc ccaaagacta tcgctccgtc acgacagcag
    36721 ttggaccaac tcctcagcga ctttcaaaca gcctatgaga gacaagattt gtcctccttg
    36781 cagcgcttgt ccgacatcag cccggaccgt cagatgtttt tggacatgat gagcaataat
    36841 tattcgatta ttagagtctc aattcagaat atttctgtca aaaacgacca agcaacggcg
    36901 acactgatcc atgaggaact cgtcgataaa aatggcgaac cggtcactcc cgatagaatc
    36961 ctgcggagtg tccgcctcac ggttcgcaaa gacggtgatc gctggagcaa ggtcctgtgg
    37021 tagagaaggt ttatctgtga atcgcaagaa cacccggggc tcacccaatc tcagaggcac
    37081 aacccaccta ccggatcgga taacaattct cgctttgtgg aggcttttgc cacgactcta
    37141 gcccggatta ttcacgaatg ccgtcgcgag gcgttcgtga acgaagcccg ccggcgtttc
    37201 tcgcaagtaa ggccgacata ggcgtactgg cagtccgtcg aggagaccga acgagaaaag
    37261 ctccgcctgg cgagaggatc ggacgacgca gcagatattc atgaacagtc cgggctaggg
    37321 ctgcacgatc ggcaatgggt tctcccccta tcccacgggt tggcagttcc cgctttgact
    37381 cgactatcct cggcggagtg tcgaaaaacg agttgctctg tcgcgtgatc ggcaaggaga
    37441 tttgaaccgc gttcggcatc gaccgcttct gatgcaattt cgacgcttga cttccgggca
    37501 aagggctaca aggtcatcac agcttgtgat ggtaaatcag ccattcggca acctagaata
    37561 attcactctt ttgatcgcaa gtccttaatt ctccaaggaa attcgcatcg tttcgatcga
    37621 tgtgcggaat aatagggtgc catcaggcct cactcttcat ctcacgaatt caagcaacgg
    37681 catccctttc cgtaggacat cggggttccc gagtagttgg ggagcctggt cgttattaaa
    37741 ttcacgtctt gccactcctt gcacctgtgg cttgaggtaa gagtaaagct cagccagatc
    37801 aatcttgttg tcctgattcg tgtcggcagc accctgcagg cccttgagga ggaaataggt
    37861 aaataaccca tgactttttt ggtggtacgt gctcgacgtt tgcgccccgg ttcccgccgt
    37921 gagcaccaca gtcttttcgc cggcgagcag gggattttca acattgatca cagccggacg
    37981 catacccttt gcgatgaccg aacgctcacc cgttccagcg aaacaggaat cgagcatcac
    38041 gacgatatct ttcgctggca acttcccgag ttgatcatag agacgcttga gggagtatcc
    38101 ggtgatctcc agaaaagtcg gatccccatc atacggaacc aagaagccct cctgcgtaat
    38161 tgggttgggc gctccatggc cggaataata gacaaggaca ctgctacccg catccactcg
    38221 attcggaagc caacgttcga tatatttttc gatgtcgctc ttcgctgctt ggtcattcac
    38281 caacatcgca acattttctt cgggaaaccc caaggtcttc gtcaagtaat ctctcataac
    38341 acgggcatcg tgagacgcaa attccacgga ggctaacccc tgccggtatt gctccacgcc
    38401 gatgacgact gcaaaccggt tatgctgcgg tttgctagcc gagagtggcg gcacatcgac
    38461 gtcacttgga ggcactatct gagccacagg ccgctgccct ttttcgccag ccggtttcat
    38521 aactctacgc gcatacgcca ccatctgggg cgaaccgacg actccccggg tcatttgcat
    38581 gaccgtatca accaaggaat cacgcagaac ggccgccgct gcattggttc gatctgaact
    38641 tgtcgcgaac atggaatctt tggtgactcg ctgtgactta aacgcctcct cttgaagaat
    38701 ttggccgttg cgatccgtca ccgtcgcacg aagcacaccc tgtccctcaa atcgatctgt
    38761 cggatcaaag aagaggtaga aaggaccggt catgtacaga aagcgtctcg tgagacagtg
    38821 gccatcaatc tcaagctcag gctcagccac ttcaatcacc acatcgtaat tgccagccgc
    38881 ctgcttcttt tcagtgatgg tatccacaga gtcaaatact tgcgagaacg cgcctatcaa
    38941 tgcattttcg agctctaacc caattggaac cgaatagctc cctgcgcagg gaacttcatg
    39001 cacacgatgg gcctgtttga gcgagtccgg aatcaccagc gcagccgata atggcatttt
    39061 tttgagcttc atcggcccgg cggctgcttg caacgacagg tttgtcggcg agcagctaga
    39121 gaaccctgca cacacaagaa tgaggatcat gaggcacaca acacttcggg cacggcgtgt
    39181 tctcataaca gtatcctttc atttaccggt tcacttcgta agctatggtt gattccgccc
    39241 actcagaatc atccaacgcc gatagtgcct tcaataagtc tgtgatgagt cccgtgtctt
    39301 tgccgtcata cacctggaag attccctcct tgaactgatc tttgatgaaa tcggtcttat
    39361 gcgtgaccgc gatcagtttg atgcgctcgg tggcttttgc cgcgccggga ggtggcacga
    39421 cgcggagtac gattccgatg gaccgcagct tgtcatcagg aaatatcaac tccttcccgg
    39481 cactgatgga accgtcttgc gcaaaccgat tgggatacag aacagtcact gagttgtctt
    39541 gaccgatcag tagtgtccca acgtttgggc ttggattact tgtaagttac tgattaggaa
    39601 cggataaaca gtgattgtcc ttggtctcga tttgaactgc gaacgccggt tctgtaaccc
    39661 tgcgaagcac gactttgcag aggtgattga tgaaccacgg caaactggtc tttgcgcagt
    39721 tgatgcagca ccttccgttg acgacgtttc gtcgatgtgt gacgcggtat cgcggggaac
    39781 gcaaggtcca attgttctcc tgtctggatc agtttctgtg catggcgttc gcgcaactga
    39841 cctttcggga gagcctgcgc gacatcgagg cgtgtctgcg cgcccagcgc tcgaagctgt
    39901 atcacctcgg gattcgttcg acgattgccc gcaatacgct cgccaatgcg aatggggtgc
    39961 gcgattggcg catctacgcc gatttcgcgc agagcctcat cggcattgcg cggccgctct
    40021 atgtcgacga accttttggt gtcgatttga aggagtcggt ctacgcgctg gatacgacga
    40081 cgatcgatct gtgcctgtcg gtgtttccgt gggcaccatt ccgctcgacc aaagcagcgg
    40141 tcaagctgca caccctgctc gatctgcgcg gcaacatccc ggccttcatc cacatcagcg
    40201 acggcaagat gcacgaggtc aacatcctcg atcagctgtt gcccgagccg ggcgcgttct
    40261 acgtgatgga tcggggattc ctcgacttcg agcgcctgca ccgctttcac gaggccggca
    40321 gcttctttgt cacccgcggc aaatcgaatc tcaaagcgca acgtcggtac tcgcacgccg
    40381 tcgaccgcgc tacggggctg atctgcgatc agaccgtcgt cctcacgggc ttttattcgc
    40441 acaaaggctt cgaggctccg ctgcgacgca ttcggctcaa ggatcccgcg acgaacaaga
    40501 ccttggtttt tctcaccaac aacttcacgt tgccagcctt cacgatcacc gaactctatc
    40561 gatgccgttg gcaagtcgag ttgttcttca aatggatcaa gcagcatctt cggatcaagg
    40621 cctttttcgg cacatcggaa aacgccgtga agtcgcaaat ctggattgcg gtgtcggtct
    40681 acgtcctggt cgccatcgtc aagaagcggc tgaagctctc gacgaccctc tatgaaatgc
    40741 tacagatctt gagcctgacc atgtttgaac gcatcccctt agatcaacta cttgcacaag
    40801 ccgtgcggga cgacgccgac cacattgcag ataaacagtt gatcttgttc gagtaacgtt
    40861 gggacactac tgtatcccta tagatacgca gtgtatccat attgtgaggg ccgcagccga
    40921 ggactcacat aggtggattg cctgagattg acggtttgta gacttcgtca cacaccttgc
    40981 ggtgggcaga acgaatggcg aggaaaaacg cacgctacgc catgttggca caaaggagtc
    41041 aatgctggac gaatcagcac actccacacg agcggtcgat ggaaccaccc ttccctacct
    41101 gctcgacgag tagaccctcg ttcgatatac tcagggccgt ccatggctca tcttcaatcg
    41161 ggtccgctcg tcacagatta tctcctatgc cccaagtaag gagatcgcgc tatgacaaca
    41221 caccatccca ccagcagcgt cgatgttgtc cttacagccg gagaatcccg cccgttggga
    41281 cgaccggctc aagacattcc atttcgcatg ttgctcatgg gaaatttcag cgggagggag
    41341 agttcgccgg aaggcgccgg ccgaaggccg ccgatcacct ggcgcccgct gcgagtggat
    41401 cgagacaacc tccagaccat gcccggcaag ctcagtgcca cacttgaggg tcccctcctc
    41461 ggagagaaca cgccccccat cacgctgagg ttcaccgatc tcgatgactt tcatcctgat
    41521 cgcctggttt gtcagataga gcccctacaa aagctctccg acctgcgccg gcgcctgacc
    41581 aacccagtca cattctctgc ggctgccgat gaagtccgcg aatggacgca gcccccgtcg
    41641 cccaccgaag cccgctcggt gggcgacggg gctccgcccc accaggcagg cgtgactggt
    41701 gcaccaatga ccagcctact cgaccaagtg ctcgaacaag cgcccatctc ggtggctgat
    41761 cttcgcctca cggaatggca atcgtatctt cagtcactca tccaaccgct cctggtccca
    41821 aaagaacatc ccctggaaaa agagctggtc gcacaggtgg atgaagccat gagtcagatc
    41881 ctgcgaaccc tgctccatca tccaacattc caagaactga gaacggcctg gttggggctc
    41941 tcatttctcg tggatcggct tgagaccgat cagccggttc agctctatct gctggatctc
    42001 cccaagagtg acctgtcagc ggatctactg cgcagcgacg atctgcaggc cacccagctc
    42061 taccggctcc tggtccaaga gactgtccac actttcggcg cgcatccatg ggcagtgatc
    42121 gggggagtct atacgtttga tcgaacgact gaagatgtgc agatgctgga acggattgcc
    42181 aaagtgtccc aggaagcggg tgccccattt ctggctgccg catcaccctg catggtggga
    42241 tgctcttcgt tcggatcaat gccggatccg gatgactggc aaagccctac ggcacatcag
    42301 gaaagccagc aacaatggga acggctgcga cggatggatc aagcgtcgta tctcggtctc
    42361 tctctgccga gattcttact ccgcctaccg ttcgggccgg aaacagaagc gattagcgcc
    42421 tttgagtttg aggaaatggc gggcatcccc gaccacgagc actattgctg gggcaacccg
    42481 atattcgcct ctcttctgct cttgggaaag gcattcttag aggagagctg gcacctccgc
    42541 cctggatcag tccaagacat tgacgggctc cccctgcatg tctatcggga tgagacgggg
    42601 gactccgtga ccacaccctg tgccgaagcc tggctcacgg aacaggcagc agagcggcta
    42661 cttgaagcag gtctgatgcc gctgctctcc tacaaaaacc aggatcgcgt ccgcctggtg
    42721 cggtttcagt cgatcgcatt gcctttgaaa ccattgctag gcaggtggag tgaggccgga
    42781 tagcgtcaca aactgagcga acgatcacgg tctggttcgt gccggcacaa agacaaaatc
    42841 tcccagccct tcatggccgg ccatatggat agggccataa gtcgggatct gctccacctt
    42901 cattcgctcg gcggcttctg cgactcgcgc ccgcaccgtc cagaataacc gctccgtttc
    42961 caacgcagac ttgttctcgg aaagcacacc taagaaggca gcggcaaaaa tcgaatgccc
    43021 cgccccgccg tcatcgagaa ccggtttggc acctccggat gtgagggtgg tgcgaattct
    43081 cctgtcagca atcgtcttca gcagcgattg cctggcctgg tcctgcacct ccgggttgag
    43141 cctggccatg gccgaacgaa cgagtttgcc ggcgaagcac gaatcggcca cgacgagcac
    43201 ttgcttggct ggaatcaact ccagcaagtc agtcacggca ggaaactcga tccactccgt
    43261 attgtccagg aggtctcctt cgatagggat ccaataacct cggtcgattt ccggctcaag
    43321 aaagccatgc cctgcataat agacgagtaa atcgtcccgc tcggagagag tcttccgata
    43381 gtcattgagc gccttcaaaa tttcttttcg actggagtct ttgagcagcc tgacatggaa
    43441 tccatattga tcctgcaaca ccttggcgac agcattggca tccgcaatgg cggtattcag
    43501 aggagtccat tggcgatagt gatcattccc gatgacaaga gcatggtact gcccggccct
    43561 tgagatactg gtgatgctct cttcttctcc cagcgcggtt gcacctgacc tcacgatcag
    43621 cttcagaccc gtctgtttgt cctgcccatc gatggcgaca agatctatat gcacaccccc
    43681 ggtgatcccc tgggttgcct gaacaggcaa cgagaatgtt ccctgttcgt ccacaaccac
    43741 cgacgcccca ttagcagtca gtgaccgaag accggcttga gcgatcacgc gtcccgtgag
    43801 acgcatcgga cgtcccggtt gaatcacgac tggaatacgg ccttgctcaa cgcgtactcc
    43861 cctggtcagg atgaccggcg gatcgacgat ctggagggaa ggcccgacaa gaccaagatc
    43921 ctgggaagga gattgccgga tcagcgaggc ttgtttttgg gccgcctcct tcaatgacag
    43981 cacctgcatc tgcagctttg ccacctcgct ctcacgggct ttgatcagtc gctcctggtt
    44041 ggtaatcaca tcgccggccg ttgacgcagg ccggttatcc ggcgttctcc cctcaccctt
    44101 caactgctgc agcttgcgcc gctcgtcatc gagcaagctg gtccgttcgg ccagccgact
    44161 aagggtctgt tcgagttctt gttgaacgat atccaattga acctgtggag ctcccgccgg
    44221 agcgctgctg ctcccgctcc ccacatcgac cagactcccc tccctcatcc cactcgcctt
    44281 ggcaaaccac tttcccgctt cgacgctatt cttttcgacg ccgatcccct gatcgtacaa
    44341 gcgtcccaaa ttcactgccg ctcggctcac gccgcgctct gcagcctgtc gataccagga
    44401 tgctgctttg atgtagtttg gctgcccatc ggcgcccttc tcatacaaaa cccccacgta
    44461 gtactgcgca tctcgatctc ctccctctgc cgcactcatc cataccttga gagccgttcc
    44521 ataatcggcc cgatctagcg cgacatactc tccaccacga atcgcgcaat cttccgctgt
    44581 cgtccggagc ggccgacggt cggtcagata ggtcacacga gtgccaagtt gcctgatctg
    44641 cccgggaaga agacaatcga ccacatcaaa cggatcggcg gatctcgggt cttttaactc
    44701 gagctttgac gtgccatcga gttgctctga cacacagccc acgagcattc ccatcagaag
    44761 ggcagtcagc ccattacgga gttttgctcg aatatgttgt ggagaggttc tcatgcgagg
    44821 cggcacattt atccctattg aggagactca cgttacccta taatactcag cgcttcaata
    44881 tgatgaagca ggtctatagt cgtgacaggg cacgtcactc cagacaatgt cgaatcggca
    44941 ccgacattcg gttaccacct atccagcagg aaggaggacc gtgcatgatg catcaacagt
    45001 cacaaggcag aaagcggaac ttcattctca ccataagcct tggcatcggt ctcctacagt
    45061 ttgttcagcc catggtctcg accgcgcagg tgttgaatca acgagtcaat gatttattgg
    45121 cgaataactg cacgggtctg ggagcgaatg tggtcggagg aaacgcactc ctgctgggcc
    45181 ccaatcttaa cgcgctctgt cagtttccga acaccggcgc tgcttcatca agtggaggtg
    45241 gagccgcctc agttcaagga tctgccgcat cgattctcaa cagagtcttg ctgcaacgac
    45301 tggatgagat tgacgaagag gaggggcagg agcataagcg gtcctcttca ctcatgttca
    45361 atccgatcgg ctcattgatg tcagggttcg tgggcaacca gtccgtgtcg tctccctttt
    45421 atgcagcgac gacaggggga ggtgggtcag cggcttcgtt caataccggt aggcggagcc
    45481 gctggaacgg ggttgggttt ttcgccagcg gactcgtgga atcactgaac cgggacatca
    45541 ccacctttca ggatggctat aaatccaata tcctcggcat cacggcgggg gccgattatc
    45601 ggttcagcaa aaaactcgtc accggtctcg ccttgagcta ttcaaatacc gatggctcct
    45661 tccgcagcgg cggcgacttc agcacaaact cctatggcgg gattttgttc gcgtcctatc
    45721 tgccaacgga caaaaccttt gtccaggtca cgggcggcta tcagcgcaac aagtacctcg
    45781 ttgcacgtac cgcctcagct gattcccgaa ccgcaggagg aacgaccgga ggaaatattg
    45841 tgaatggcat cgcctccagc aattccaacg gcgatgtctt caacctcggg ctcatgacgg
    45901 gatacgacca tccgatcggc cggttcacca tcggccctcg tctcggcgtt aactacagcc
    45961 atacccaaat tcataattac agagaaaccg gtgggaccgg aatcgaactc aagtacgacg
    46021 accagagtat caattcaatg caaagcgtgc tcggcgtcca gggctcagcc gcgttcagca
    46081 caggttttgg cgtcctggtt ccgcagttca atgcggatta catccatgag tttgccaatt
    46141 cgcagcgttt tatcggcgtc caatttgcag aagacttgcg cgctacgccg accaggttca
    46201 cgttccaaaa tgatgtccct gttcggaact acttcaacct cgggactggc ctcgtgatgg
    46261 tcttgcccaa tggctggcag ccgttcgtga acttccgggc gatggtgggc aacgaacagt
    46321 ttaataatta cgcaggaaca ttcgggctgc ggattgaact ctaaggcagt ggctggcgca
    46381 cgatgaaaat cgaggtagag acagtggagg attcttcagc gtggtggagc cccgaggctc
    46441 gttccctgtt ggaggcaagt cctgaggcgc gattcccaat ctgattgaga tttggcattc
    46501 cgcccgtgat tggtctcgtc gtcatattgc ttggaaagag attttgccca ggcgtcatag
    46561 gcttcaaaaa gtctcgatac ttcccgctgc aattcgtcgg tcgtactcac ccgcaacgct
    46621 ccgagcgcct tcaccaaatc acgcgccacc aggccggtga tgtcgaagtg tccctgctca
    46681 tgagccaaca gcgtatcgct cttttgattt gccacgaccc atgagttgtc ccgaatgacg
    46741 ctgagtttga ccgtgaaatt ccccagccgg aattgtccct gctcgcgcgc gactcccacc
    46801 cgctccggtt gcagggtctg tgtatcaatt tgcgcatcct cggtctcgcc ggccggacga
    46861 gtggccacat ccttaaactc atcccaggaa atctgcctag gccaaccggt cagccgaggc
    46921 cccgtggtcc ctccctcatt caggccgagc ggaccgggag taccgaatcc gccgatcggg
    46981 aaatgcggat caaaaggccc gtgcaacggc ctggtatctg acccgattgg tcctggtgtg
    47041 cgtcctccgc ccattgcgaa acctccctct tgcaattgtg gaagccgggg gccgtgcgat
    47101 tagagatcgg ctatccggtt ggcgcagccg ataaggacaa agccatggga ctatcgcgct
    47161 tgttaaatac gactccatcc tcgcgttaca tttcacaggg aaacctgccg gaggcgatgt
    47221 atttagcgca cctccggcca acccttcgag accggcagcg gggtaaaatc gagaaacttg
    47281 aacgcggttc caaacagcga gcccccgcga ataatgcccg gcacatagag ctggtaaatt
    47341 tcatgaatgc tttgcatagg cgtcatggtg ggcagatcac gcggatcctt gccagcactc
    47401 ctcacctgtt tccctctgcc cttccaacac cggaggggct ggaggaccct agcccgcacg
    47461 tatccgttca cctcactgta ttcatagagg atggcggcaa acagccgggc cgcgtagctc
    47521 agcgaatagc catgctgtaa agcgaaatgc ttgatctttt ggaagtgctc gtattccagc
    47581 caccagggac cattggcgcc ggtttcggga ctaccgttag ttgtatcaat gaaccgatac
    47641 agcacaaggt caggggccaa atcgtattcc cgcaccatac tcatgccctc taacgctttg
    47701 tggtagccct gactcatcaa atcctgattg atccacttgg ccgtgtcgcg ctgcacgatc
    47761 ggacctccac tcgccagagt tggttgttct actctaccag gagctgatgt cgacaaaaac
    47821 tgaatatccc gcgatcatcg ttctcagtcc cctgttccgc cgcgcctgct gccggatgcc
    47881 cgggggcggc gtggttgaag agataccata gaatgagatc gtactcgaaa atatccctta
    47941 tcccgtcctc aaccccagcg tgcttcgacc ttgcataaaa tctcatattg agatatcatc
    48001 atgccgtgca cgtcattacc cgaaggaggc tgatcgaatt cgcgcacaaa catccgcctg
    48061 cgcgtccccc gctgcttcac tggtttgcca ttgtcaggaa aaccgattat tcctccttcg
    48121 ccgacttacg gatcacattt ccctccgcgg accaagtggg gcagttcacg gccttcaaca
    48181 tcggagggaa taaataccgt ctgattgttg ccattcatta caatcgaaag aaggtgtaca
    48241 tccggcacgt gctgacccac gccgagtacg accgtggcac atggaggaaa taattcatgc
    48301 tagcaaaata catccatgag gcgaacgccg aataccggcg gattcgtaaa cgggttcccc
    48361 tcggggtgtt gcactccgtg gaagagtacg accatgcagt tgccgtcctc gacgatattc
    48421 tggacgaaat cggccaagac gagaaccatt ccctcaccga cctggccgag atgttggccc
    48481 tgtccatcga gtcgtatgaa gaagtccatg tccccgttcc tgactcttcc ggacctcaga
    48541 ttctgagata ccttatggag gaacatgggc tcaaccaatc tgatttactc gagattggaa
    48601 gccaaggcgt cgtttcagaa atcctttccg gaactcgtga gctgaacgtt cgccaaatcg
    48661 cccgcctggc tggacggttc ggtgtctcgc ctgccctgtt cattccgacc ctaaaagcaa
    48721 aaaaccgagc gatggctcgc aaaagacaat aagcatgaga caagcttagc tcccacgggc
    48781 aacatcctcg ccgcccggtt tacgaaacgc ggcaacaagg tacgcgtcat cggctcagcc
    48841 gaatggagaa agtattgggt tttaccatga aaccacccaa tctccagtca ccggctcagg
    48901 tggacagctc ctcaacctgc acacaaactc tcagaaacga ctcccgaccc cattttttct
    48961 acccttggca gcatgcggga ccacgggtga gtgttgttca ttggagtgga accttcgaga
    49021 tgggcgacct tttttcgcct cccctccatt cctctcaggc cgatatcact cccgtacaac
    49081 cagcgcgatc ttgtcggaat aaattccatg aattcgattc tcagtgccta ctgtgatggt
    49141 gtagaaaacc atcgttacaa gaagttcgcc gtgctccggg tgcttttcca gaaaccaagt
    49201 ctttatgaat gcacctccgt cagccattag taccactaac tgtgatgcgc tcacggagcc
    49261 tgagtcagcc aatcgcatca gtccgtcgat ggtcgatgtg gcgaccgtga ttgccgtgcc
    49321 tacaactgga ataagacttg ctggcccgag gatccacggg attgatcctc gggccgcttg
    49381 acgttggaat attcccgcat aggttttcga agtaccctta ttcaacacat atttatccac
    49441 tacatacttt ttatcgaaag agagcttgct gcgttccgcc gtcgacaaca ctgcagaaat
    49501 ttttccttgg ctcaccaagc cctctattcg caagccagtc cttggaaacg ccacgctgag
    49561 ctgaggcctg aaaaacaatg gatcgtgttg ctcaaagtag ccagccatct gtagcctcct
    49621 caaggcaccc aaaaccactg cacgcgatac ggtttgactt agggaccgtc ggatcattcg
    49681 aattcgaatt cgaactcgaa ctgaacagcc gctccgctgt ccgccgcacc caagcttcac
    49741 tccaagaagg cgttgcggac ctctcgcaaa aaggcgcctc cctttaatac ggttttgcgc
    49801 ctaacagcgc ctgctgccgg aagcgtgggc gctcgtgttg aagagtggtg tgcctggttg
    49861 gcgcattgcg aggtcgatgg cggcgccacg ttgacattgc cggcctgttc ggagcacctg
    49921 gctaaacgca tcgtgcttcg tgtcatctcc ccgcatcttt tcagaatctc ccaaacagta
    49981 ggtttttatc gacttggctt tgcaccatca cgtaagatgg atgcatagtc gccaggacca
    50041 tcgacgactt ttccgaaagg ggctcacatg gagcgtatcg tcaacctaca tatcgaaaag
    50101 cttccagaag gcgtttacct tgcgacatcc gacgcaatac aggggctcgt ggtacaaggg
    50161 cgaacgatcc aagaaaccat tgagatcgct cgtgatgtcg ccaaaaagat tttagagggc
    50221 aaacaggaac gcgaaggaga accgcctctt ccacctgccc gtgatgaatt cgattacccg
    50281 ctcatcgtca atgcctaatg ggacgactgg ccgggtttaa gtatcgggaa atcgtccaac
    50341 ggctcaaagt tctcggattt cagttcgatc gtcaggctgc aggtagccac gaaatctggt
    50401 tcaacccttc cattcatcgg tacaccacca ttcccaatca tcctggcgac atgcctgagg
    50461 gcaccctccg ggccatcctc aaacaggctg gaatcacacc ggccgatttt ctagacacgt
    50521 aagaggctgt cgccaatcgc tcgctgagcg cagtcgctgg ctcatgaaac atctcaccat
    50581 cattcctgtc actgcaaacc gagcagcgcc tgctggcgga atcctggagc gctggtgttg
    50641 aagagcggct ggcctgattg gcgcatggcg agttcgatcg ctgctgcgat acgttggcgc
    50701 tgcttgccgg ttcggagaac ttggttgagc gattcaattg tcatcggctg gccacagaga
    50761 taacgcgtcc cgttcgtgaa caggacgcgg tgggtgaacc accacttttc aatcaactgg
    50821 agattgggcc aaggtaagtc atcgtccgga tccatgtcca cgttctcgtc ttctggattc
    50881 tccgttggcc cggcctcgaa accctccggc ttgtcggtat ccaagtcgtc ataggcgagg
    50941 tcgattccgg taataaaagt gaacgattca cctgccactc gcgccaagac gggatcagcc
    51001 atctgttgaa tcaaccaagg aatgtttgcg ggatcgccga tgacgcccat cgcttgaaca
    51061 gccatacgaa gcctgctcgt actgcaggcc aaatgcctct gccaatcctt tgcctggtct
    51121 aatggcattc gccgtagcgc cgttgcacag gccttcccag ccctggcatc acctgtctcc
    51181 gcgaagcgtt gtaatacttg aatcgcagcc ggttctccaa gtaaggcgcc ggaccaggca
    51241 gcccagaagc gacaggcggt atcttgctca gtaagattgg ttcgcagaaa tggaaggaga
    51301 tcttttctgc cgagttcccc aactgactta agcgcacgag ccttcagtaa aagatcaatc
    51361 gagttcagag catcgttcag cgcagcacaa ggatcctgac gatgcgccgc agaggcggca
    51421 agaccaatcc ccttctgctg ggatgattca gccgtcagca gtcgctgaat gtgtggtgca
    51481 acttgctgaa agggaatcca ccctagcccc gaaaccaaag ccttgg
//
LOCUS       DAORRQ010000028        49648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_40_length_49648_cov_556.005908, whole genome shotgun sequence.
ACCESSION   DAORRQ010000028 DAORRQ010000000
VERSION     DAORRQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 49648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49648
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40_length_49648_cov_556.005908"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1875
                     /gene="ppk1"
                     /locus_tag="PK224_10510"
     CDS             <1..1875
                     /gene="ppk1"
                     /locus_tag="PK224_10510"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRB16212.1"
                     /translation="VTHGVEQRGADGLTPSELLARIAALTHQLVHEQYVVLNQSLIPQ
                     LADQRIRFLKRADWMPSHIRWMRRFFSRELLPLLSPVGLDPAHPFPKLLNKSLNFLVT
                     LEGTDAFGRPNGTAIVQVPRSLPRVIHLPVRNAAWPHDFAFLSSVIHAFVHQLFPGMH
                     VTGCYQFRVTRNSNLFVDEEDVDDLRRALEGQLPERRFGDEVRLEVADNCPPDLVYFL
                     REQFHLDARDVYQCHGPVNLHRLMAVPDLVDRPDLKFQSFTPGIPTTPVPSEDWFDAI
                     RQGDILLHHPYQSFAPVTEFLRQAATDPHVLTIKQTLYRTGADSAIVQSLVDAARGGK
                     EVTVVIELRARFDEEANIELAHDLEEAGAHVVYGVVGHKTHAKMSLVLRREGRMLRSY
                     THLGTGNYHARNARLYTDFGLLTCDAAIGRDVRTVFQQLTSLGRPGRLKHLLQSPFTL
                     HATLLKWIGRETDRAKQGRPAHIIAKMNALLEPQIIRALYKASQAGVKIDLIVRGPCA
                     LRPGIAGLSEHIRVRSIIGRFLEHSRIFYFLNDGDDRVFLSSADWMDRNFFRRIEVAF
                     PVLDKTLRQRVIDEGLRPYLEDNTHAWILHRDGTYRRQTPGRRAARSSQQWLMNKLVT
                     "
     gene            complement(3327..4079)
                     /locus_tag="PK224_10515"
     CDS             complement(3327..4079)
                     /locus_tag="PK224_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16213.1"
                     /translation="MNRTVIALLTLVVLTTGACASKPPKKQFQRLAGEFVSPVHITKP
                     VDQQSAVLGITLKSPAWVPNTEDRLYLVKVENERDLFEGSKLIPTSILAPPPGAGGGG
                     TVYVQNVPPGRYAAVAFSTSEKGFGKVREVTLFLLPKTVVKQSDTTVVSGSISFMGEY
                     EVGTSTMVLQEKAADPVQEHYFEAFWGKPLAQVIADLQMIGTPAYFAVHTVSVKQGSR
                     DVAAEERFLAAAKRDLEPAWAPVIERRRTAVK"
     gene            complement(4298..5560)
                     /locus_tag="PK224_10520"
     CDS             complement(4298..5560)
                     /locus_tag="PK224_10520"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16214.1"
                     /translation="MNIGLNARALTIGLAFLGVVSGCAVDQRWRSAMQEGNHALRSGD
                     YTHAEESFVAARKEAEVLDPQGKRLAETLSQLGEVNRELGRFPRAEALFQEALAIRER
                     VYGPAHAETAASLTDLGELYRLQGLYTQSEALHQRAREIREQVFGADHSKTAESLNNI
                     AVVYQDQRRFADAEPVLQRALGILEKQLGPEHSTTAITRDNLAKMYQAQGQHARAMPL
                     YQRALTIHEKAFGPNHPIVARNLENLADTYRAQNQYPQAEVLYQRAVSIFKKSLGNDH
                     VDTAEAMSRLGQLYELQGLYSQAEPLFQQAIAIREKQQGAESPHVAGELKNLAGLYQS
                     QNKLEQSEDLYKQSLVIYEQSVGYDREAYVKALKSYASLLRRRQRSGEAERLEERAKA
                     VLKRLSLESQSQGKTAAEVPLAPVSPLP"
     gene            complement(5801..7816)
                     /locus_tag="PK224_10525"
     CDS             complement(5801..7816)
                     /locus_tag="PK224_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB16215.1"
                     /translation="MANSTLTTPDSRPLRIALIGAGRHAQHHARAILRCPGVQLVAVA
                     DPSDAAQAAMRDIVPGIGCFKTPEELFASERLNVVHIITPPASHAPLARMAIKAGCHI
                     YVEKPFTESVEDAQQILDEASAKGLRVCAGHQLLYEPPTRVLTQYLPSIGRVVHVESY
                     FSFRTVRHAPGGRKVLRADHQLLDILPHPVYLLLQVLEQAGEGRTELLSLEVSQAGTV
                     HALVRRGGVTGTLIVTLEGRPVESYLRVVGKNGSLFADYVRSTTQRAIGPGSSGIDKL
                     FAPYRQAWQLLIGTTSAMANRFLKSQRSYPGLAELFTAFYESARTGGPSPLSPESLLE
                     TVRICERVAKALKVGEAKALAAAAPKPVESRGVLVTGGTGFLGKEIVRTLLSRGRPVR
                     VVARREPSPWERIAGAEYVVADVATGAAAQLFKGVDTVIHAAAETAGGWPEHQRNSLD
                     ATEQMVRGADAAGITHFVHVSSLAVLAQGNGRPIGDDHPLEPDSKGSGPYVWGKLESE
                     RLAVQLGKELGLSVKVIRPGALVDYRDFDPPGRLGKRLGNIFVAVGSPGDRLGVVDVG
                     FAGRFLGWMTDAWDNVPSPLNLLDPVSPTKQELLDRLRQANPDLTVVWLPRFVLVPLS
                     WLATLAQKILRPGKPAIDVAKVFSVLPYDTSGIAKLAPQVDHASEKR"
     gene            complement(7963..8949)
                     /locus_tag="PK224_10530"
     CDS             complement(7963..8949)
                     /locus_tag="PK224_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB16216.1"
                     /translation="MEPASILIVEDEPVVAKDIQLSLQRLGYRVPATATSGEEAIRKA
                     IDMHPDLILMDIVLKGKMDGVETALQIQRKQDVPVIYLTAYADNHTLERAKVTSPAGY
                     MLKPYQANELRTTIELALHRAQHDRHMRERLRWIATTVRCIGDGIVTTDRGGRVTYMN
                     PAAEEFTGWSQEEATGMSVSALLGSEAVPPSESPAQQAMAQVCMVTIDGATLISRLGA
                     CRTIRGSVAPVVDDGGIVLGAVLVFHEIASSGQGLQVQGGQREGFWMMDAQLGRPQGI
                     INLCSWCKRVPDESGEWYDLATFIAERSAIQFNGGLCPECMDQCFPCDERHK"
     gene            complement(8955..10787)
                     /locus_tag="PK224_10535"
     CDS             complement(8955..10787)
                     /locus_tag="PK224_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB16217.1"
                     /translation="MTIPASFFLGMSRSSYSRLGVALALIAMIGAIDWWLPLGLTITT
                     LYVVPVLIASRIPHPRLTFWVAALASLVTILDMFDRPLFALTWVSAMNRAFALMVIWV
                     TALLCLRRQRDEAELLRINEDIEQQVQERTADLAAANQELEVLRAEAVSELVAIVKSS
                     DDAIVGMTLNGMIQSWNRGAERVYGYRAEEVLGRPISVLCPPNRLDEVPTMLDRIARG
                     EHVRNVEMVQRRKQGERIDVSLTISPVKDADGCVMGASAIARDVTVKRRIEAALRESE
                     ARFRMMADTAPVMVWMAGPDTHITFINKRWLEFTGRTVQEEIGDNWFTGIHADDLDRC
                     RKSYLQAFKSEQPFFLEYRLRRHDGEYRWIMDTGVPLFDEEGQFGGYIGTCMDLTERK
                     DMEDQLRRMLKEKESLLREVHHRVKNNLQVISSLLNLQSASIKDPVVNQLFRECQVRI
                     TSIALLHETLHRSHDLSRIKMGDYIRTLTGHLFRSYGVDPNLISLELNVDDVEFDIDT
                     GLTCGLIIDELVSNCLKHAFIDDSGGTVHIDLLDHVDGTFTLCVSDNGIGIPKDGVLN
                     NPDSLGLELVTLLAEKLDGSTELRSGAGTEWQIRFQQLQYSERV"
     gene            complement(11321..12439)
                     /locus_tag="PK224_10540"
     CDS             complement(11321..12439)
                     /locus_tag="PK224_10540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB16218.1"
                     /translation="MNIPLLDLKAQYQSMRSEILAAIEATCDEQGFILGPRVVALEQA
                     VATYTGSAHGVGVASGSDALLLALMALGVKAGDEVVTVPFTFFATAGAISRLGAKPVF
                     IDIRPDDFNMDPALLERAITKQTKAIIPVHLFGQCAEMDAINEIARRHKIGVIEDACQ
                     AIGARQNGRHAGVLGDVACFSFFPSKNLGGFGDAGMVTTNDKALSESIAMLRVHGSRV
                     RYVHEAIGINSRLDALQAAVLLVKLKRLDEWAEGRRRNAARYVQLFTEAKLTDRIALP
                     VVGANNFHVFNQFTIRVQKRDELRSYLKDQGVGSEVYYPLPLHLQNCYRDLGYQKGAF
                     PQSERAAEEVLSLPIYAELSDEQLQYVVQAIASFYRKR"
     gene            complement(12462..13652)
                     /locus_tag="PK224_10545"
     CDS             complement(12462..13652)
                     /locus_tag="PK224_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB16219.1"
                     /translation="MQVVCHVITKLELGGAQEVAMQVVAGLDRRRFRPVLLAGPGGLR
                     TDDAHALEGVEVGTIPSLVREIRPLQDLCALWELVGAFRRLRPKIVHTHSSKAGILGR
                     LAAWLAGVPCILHTIHGYGVTPVQPFWQQQALIALEWMVGRITTHWIAVSHADRRQGI
                     EWGLFTASKVSVVRPGIDPTAFAVRIEPQERDRLRALLGVGPDQLLVGTVSCLKPQKS
                     PEDFVRVAALVCQRVPAARCVLVGDGALRPKVEALLLAQGLQDRVTLLGWRRDVAALL
                     KAFDVFVLTSQWEGLPCAILEARASRIPIVATRVGGAAEAIVEGIQGTLCPAGDVRAM
                     AERVCQILEDERFRADLRAGTQELPEEFTIQETLKQYQSLYAYLLHATRPSEDVMKLQ
                     PNRL"
     gene            complement(13666..14976)
                     /locus_tag="PK224_10550"
     CDS             complement(13666..14976)
                     /locus_tag="PK224_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB16220.1"
                     /translation="MILIVGAGLAGLSAAYHLRGMPYKILEREREVGGLCRSYVKDGF
                     TFDYTGHLLHFRQTAIKALVESLLPDQLQRHARKSYIYSHDTYTEYPFQVNTHGLPPE
                     VVRECLLGFIATLSNPSPKPPVEGRSFKQWIVESLGEGIAKHFMVPFNEKLWQVPLDE
                     LTSDWVSWLVPKPDVKDVVNGALGIKDKAFGYNPSFQYPASGGIKVLPEAFLPSVENL
                     SYDSELVEIETGRRRAVFRSAQGEWTQAYDRIVSTIPLPDLVRRCVDLPPSLRELAAS
                     LRWVSVYNVNLAVARERISDKHWIYFPEHRYPFYRAGFPMNFSPAMGQPGCSSLYVEI
                     SHQPTQRESDAVLIDRVRRGLEQAGVLQRTDELVMSDVKDLYYAYVLFDRYRGRAVQE
                     LLAELERRGISSIGRYGLWEHTSMEDAIAQGQQAAARLRMKAAA"
     gene            complement(14973..16031)
                     /locus_tag="PK224_10555"
     CDS             complement(14973..16031)
                     /locus_tag="PK224_10555"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRB16221.1"
                     /translation="MILLTLTFVVAMLLSIYGVPIARRAALKFGIVDNPDGRLKHQRE
                     PVPYLGGLAIYLAFLVSLAFTFEFRQDVLGIVLSGTLIVMLGLIDDFGVLSPGTKLAG
                     QFLAVFVLIKSGIRIEIASLPDWVDIVLTVFWMIGIINAFNLLDIMDGLSAGVGVISA
                     AFLCVVAILNGDQAIAFMLAALMGSLLGFLRYNWRPASIYMGDSGAMFIGLMLGALSM
                     IGKYTSGHSVSLLTPVLILGMPIFDTLFVMYIRFLRGLPVFLGSPDHLAIRLRHWGLS
                     VTQVVLLSYLGAALLGGIGLLVMAMPQDLAVVLSGVTMLCLASAAVALTRVNLSTGTV
                     AVSAEAASVRSTERTDAV"
     gene            complement(16028..17164)
                     /locus_tag="PK224_10560"
     CDS             complement(16028..17164)
                     /locus_tag="PK224_10560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB16222.1"
                     /translation="MTVTYFSKSSSIGPSSRYRVFQFLPHFQAAGIGCRVEALFGETY
                     FSILKVHPRALRTLLKIPYVLICFLRRLWTLLTLGKRDLIVIEGQLFPYAPPLAERLL
                     RWCRYRVAIEMDDAIYLTPGHEKKIPALLSMATGAIVGNDRLAAYAKQFSPRVCVVPT
                     VVDTERFKPDSTRSTGSSAQNSDAITIVWIGLAYNLKYLDVLAPALRALQSRFRLTLR
                     VVCSRPPHMPGVEIEFRPWDFQREVADLQDATIGVMPLEDTEWARGKCGLKLLQYLAV
                     GVPSVASPVGVNRDIIANGENGFLAATEQEWYERLETLCRQPQLRARMGQAGRRTVEE
                     QYSLAVWGPRLGDVYRTLAGNEHVNEIHRAVHSPVSGHRTTGTR"
     gene            complement(17349..18914)
                     /locus_tag="PK224_10565"
     CDS             complement(17349..18914)
                     /locus_tag="PK224_10565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16223.1"
                     /translation="MPTPPHITIVLVVSRIDEKRLRETIESLIAQTYPNWTLSVVGEV
                     SRLRMMEHIINAYSEADSRIQPEEISEKERISDRILKGRLGEFVSFLSLECYLAPEAM
                     SSVITRFQECPDLDFLYADEDMVRENGEFLDPFFKPCWSPDLLIGMNYVGRFNVMRKQ
                     LLLDIGADWGDIANDGFYHLVLRVAEETNKIGRVPQVLSHCHITDESSDITAGQSELD
                     VTAQMKSLQDALNRRGESADVVRDGAGKIKVHYRVSRNPLVSIIIPTKDRWDMLRQCI
                     KSVESVTRYPSYEIVVVDNGSRTAKAKEYLSTLSKKWIIHRFPGPFNFSEINNYGASK
                     ANGEFLLFLNDDTQVIAPEWMSAMVAQAQRTGVGAVGAKLLYPSGMIQHAGVVLGIRG
                     VAGHAFRHLRGDTQHYRGLPHMLRNCSAVTAACMLVSRDVFRQVNGFDERLAVEFNDV
                     DLCLRIQQQGYRIVYTPEAELYHHENATRRGRRAPDDEARFRLKWGEFIMKGDPYYNP
                     NLTQLREDWSLKL"
     gene            complement(19357..20643)
                     /locus_tag="PK224_10570"
     CDS             complement(19357..20643)
                     /locus_tag="PK224_10570"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HRB16224.1"
                     /translation="MAGICFNGDNHPGPRLIAFFLTQFHPIPENDEWWGKGFTEWTNV
                     TKASPLFDGHYQPHLPTDLGFYDLRLRESRHEQIRLAKQYGIDGFCYHYYWFSGTRLL
                     NRPLDDMLVDRESDMPFCLCWANENWTRRWDAAEHQVLISQKYLPDDDLNFIKSLVPF
                     FKDPRYIKLRGEPFLIVYRPQHLPNPRSTVQVWRDYCETVGIGKIHICAALTHGNEQY
                     GQFGFDSGVEFPPHNLNCRNVNDRIEFHDQFYGNAMEYQDIAQTYLTRQYRESNVFKT
                     VFPSWDNTARSRERALVVLNGTPHNYEHWLAKTLDQTVNTYPNEDNLVFINAWNEWAE
                     GCHLEPDRRFKLEFLEATLRAKNNLSTIQGFQSLRAFAGDGVVRRSIYGDLLSIARYH
                     GWRTITTWRYWINQYPRLKDFIKMLISPIRRLIANG"
     gene            complement(20698..21543)
                     /locus_tag="PK224_10575"
     CDS             complement(20698..21543)
                     /locus_tag="PK224_10575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB16225.1"
                     /translation="MSTKPRHKYEFSVDLTAPSAPTYVVRMVGHFKRVLEVGCGPGSV
                     TKLLTQHGQCRVTGLDLDSEAIDRVAPYCEAIVRGDLNSPEWPSLLDKVKPFDVVVAA
                     DVLEHLYDPWSVLAQMAGFIRPDGYLVISLPHVGHAAVASCLINGDFEYREWGLLDRT
                     HIRFFGLKNIQDLFTQAGLKILEAEYVVKTPEETEFAASWCRLSAAVQDALKSSDYAD
                     IYQVVVKAVPISHPGYAVSLVPPEPRHTAVGMRTLWKTRLAHHLSQSTRQRIRQGLSL
                     LGIRL"
     gene            complement(21503..22288)
                     /locus_tag="PK224_10580"
     CDS             complement(21503..22288)
                     /locus_tag="PK224_10580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRB16226.1"
                     /translation="MGGGWSVGVEVIFYAIFPVLLLLVKPHKPSLLLLLLLVVSIIVS
                     GALRSVLHLQGSNVESSSKFDWSYFSFPSNICFFLMGLYVYLVVKFHGRHSTLLRVHA
                     PIATTAIIAGLLFSDVGLLIYSSARWDIVLWGFGLSALCAWQANRPSRVIASGVLQYF
                     GERSYSIYLMHPVIIFALKSYLLKIYTLCFPAIGAYSYFICSSILIVSILPCVEITYR
                     LIEVPGINLGRRLIASKRIAVITPTLDLESHEYQASSQVRILS"
     gene            complement(22731..23366)
                     /locus_tag="PK224_10585"
     CDS             complement(22731..23366)
                     /locus_tag="PK224_10585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB16227.1"
                     /translation="MIEKLMTVLAGQKICNVFDIGIFKGGSVALYDQLFPTEKIVAVE
                     FMSRPVDALTQYIHRHKKADHIKPYYGVNQADRRAMRKILAYEFPNQEIDLIIDDASH
                     LYEETREAFNISFPYLKPGGHYVIEDWAWAHWPGDYWQKDNPHFKDKRALSNLLIELT
                     MLAASRCDLIEDIFVNHNYVIVKRGVSKLSPDGFDIGEHYLLRGKQFGAWL"
     gene            complement(23504..24634)
                     /locus_tag="PK224_10590"
     CDS             complement(23504..24634)
                     /locus_tag="PK224_10590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRB16228.1"
                     /translation="MNAELVGSQDKLMRVRNEYLDLMQAFLTGSIYRDFAQAPFGSNL
                     FDSHRREHGLDWPSTAETMIGVKRLANLRALTESVIADNVPGDLIETGVWRGGACIFM
                     RAILYANSVSDKSVWVADSFEGLPAGNTLQYPADAGSDFHTYSQLAVSLEEVKENFRA
                     HGLLDAQVKFVKGWFKDSLPKAPIDRLALLRLDGDMYESTMDALTYLYPKLSRLGYVI
                     VDDYHVVPACKAAVSDYCEKNAIRPQIVEIDGVGVYWRKSSVPEGQSEIVSTNEVVAS
                     FDVQIARLDQAVLELSKRAIAQLNRCLSERDELIASMTQSLAEQDRQIVNLKQTLVQR
                     DEYIAALLSSASWRVTEPLRVMGALLRKFHPLGHTNLKGREK"
     gene            complement(24696..25961)
                     /locus_tag="PK224_10595"
     CDS             complement(24696..25961)
                     /locus_tag="PK224_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB16229.1"
                     /translation="MPYFREFWALKNCSLEIRKGETVGIVGGNGSGKSTLLQLICGTL
                     MPTNGGVEVHGRIAALLELGAGFHPEFTGRENVYMNASILGLSQTEIDAKYDEIVAFA
                     EIGDFIDQPVKTYSSGMFVRLAFAVIAHVDAEILVIDEALAVGDVFFAQKCMRFLRTF
                     KSYGTIVLVTHDFGTVVGLCDRAIWLEQGEVRSIGQAKQVCEEYLANRYGAVRSTIPR
                     QVPEVSMSNAEQRSLSKHDARIDFINHSNLRNDIEVVDLSAERHGFGNGGASITDVHV
                     TDLEGRQLSWVVGGEMVRLEIEALVTVPCKNTIIGFQFKDRLGQVLFAQNSFTAYCMN
                     PVSASPNDTVKGVFVFRLPILPRGKYSVDVAIADGASPESVTQLQWLHDAFSLESHTS
                     SVVSGLVGLVFDSIELSSQGTPFSKTAVA"
     gene            complement(26077..26868)
                     /locus_tag="PK224_10600"
     CDS             complement(26077..26868)
                     /locus_tag="PK224_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB16230.1"
                     /translation="MVFTSLYSHWNLIRLLTGREIASRYRGSLLGVLWSLAHPIMMLV
                     VYAFVFRVVFKASWSHGGEGNSEFVMTLFTGLIVCAVFNECVNRAPTLILNHVSYVKK
                     IVFPLEILPWVSLGEAFLNMGVSITVLLVFHISSHLPLHWTATMLPLLVIPLALYALG
                     VSWFLASVGVFVRDVGHATTIVTNLMLFLAPVFYPLSSLPDAYRPFMYLNPLTFVVEQ
                     SRNVLIMGRMPSWHWVVIYFLCSAVVAWLGLAWFQRTRKGFADVL"
     gene            complement(27024..27788)
                     /locus_tag="PK224_10605"
     CDS             complement(27024..27788)
                     /locus_tag="PK224_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRB16231.1"
                     /translation="MGAIGVIALNAFRESLRDKILYNLVLFAGLLIGLSVLLADLSIT
                     EHHKVIVDMGLAAINLIGVIIAIFVGISLVNKEIERRTIYTIMARPISRASFIMGKYL
                     GLALTLFVNMAIMWAVFLLTLWLYHVSIQPSIFQAVALIFVEILVVTAIALFFSTFTS
                     TTLSAIFTLGLYVIGHLTADLRAMVANSDSGPIKSLVDLLYYLCPNLEMLNIKGQAAV
                     GVVAAPEYVMLASLYGVMYAGVLLISACVVFQQRDF"
     gene            complement(27788..28759)
                     /locus_tag="PK224_10610"
     CDS             complement(27788..28759)
                     /locus_tag="PK224_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB16232.1"
                     /translation="MDDIVTDNLTKSYASGWPGRPPFVALDGLSLTVRRGEIFGFLGP
                     NGAGKTTTLKILLGLVRATSGQAYLLGQPAGDVAARRRIGFLPESPYFYDYLTAEEFL
                     GFYGQLAGLARSAITQRVTDLLGLVGLTDARTRQLRKFSKGMLQRVGLAQALIHDPEL
                     IILDEPMTGLDPVGRKQVRDLILNLRDRGKTVFFSTHILHDVEMICDRVGIVMKGRLV
                     ASGRVDELVRQDHTQSVEVVCQQLKVEENAFIHSLATRVLQQGQQCLIVLPSPDAVDA
                     LVGEIRRQGGRLLSVTPHKASLEDLFFQEATQRTSPSLPHVTSGRAS"
     gene            complement(28775..29698)
                     /locus_tag="PK224_10615"
     CDS             complement(28775..29698)
                     /locus_tag="PK224_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16233.1"
                     /translation="MTQVVCGLALLAGASGVLHVLDQERTSVALAEQLAYLPKGDYLK
                     LAVLGYRQVVADLIWLQAVQHIGAKRDTQAGYMWTYHAVDVLTDLDPTFVPPYQATGL
                     FLGVLVGRHEEGVAILKKGIQHNPSHWQLPFLAGYISYYERCDPAAGGEFFRIAARVP
                     GAPAYLPQLAARMTVESGDSSAALEFLERFSRSVTDERVREALFRRMKEIVQEQDLRV
                     LEEGISRYRAHYGQAPAKLEDLLLRGIITSLPMDPLGGQYELNSLTGTVSASSTRERL
                     RIHEKVACRLGRATQSEGGAGSMETPLPTVQ"
     gene            complement(29734..31731)
                     /locus_tag="PK224_10620"
     CDS             complement(29734..31731)
                     /locus_tag="PK224_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB16234.1"
                     /translation="MQFLSSIPLVVTSALVIFSPLQEGGTTHVAQMIIRFLILAWAGG
                     ALALSIRQGRLTIPLLSVRYFVLVFLGFAVIATMFSPYAHPSRQWLFTIICYATLFYL
                     LVSFVDRWEHVHTLTIVVVLMGVGEAALTILQGLVWNDMRPSGTFFNPNFLAGYLAVS
                     WAILLSAAVYGYRRTAALHWLPISPTLWWFGVGASLGSLFLAVLLTQSRGGMIVVLVA
                     TVFIVMARYGWKVAGSCLVLLMVGGLLIPTPLRERVQAEHQQNPVSYARLQMWQGALT
                     QMAEHPLGIGLGLYQYTYPRYAFPVEGEIARYGKVAQTPHNDYLQMGIEMGVGALLLF
                     LAGVFMVGRDFARVFQTRLSRRQRSLILGLGAGGIALLVHAALDSSLRETALAILLVL
                     CAGLIVSAARMTRCSTDAVQVIPIRSRFVWGMSAACVLLLVGAEVSRLGMAWLTFDAA
                     SRRAVAGETDAAIQGLQHAIALDPGKSLYHHGLGSVYARAFDASRDKEMFRLAHAEFK
                     EAIELNPLDSRLLGLLGQLYVSAAQAPSLSGGLDEQRKSWIRAALRVYERAVRLAPFS
                     APYRYEQARLHWMLGEKAAAEQQAKEAERLEPNFLPARALLAHVWVDAGRIDEARQEL
                     HEIQARQARYEQWNKNSIDRAFLGVDVAPLRAAVGEKGLAG"
     gene            complement(31951..32481)
                     /locus_tag="PK224_10625"
     CDS             complement(31951..32481)
                     /locus_tag="PK224_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB16235.1"
                     /translation="MLKQIKGQKGFTLIELMIVVAIIGILAAIAIPNFLRYQAKSRQS
                     EAKTNLGAIFVAETAYLSENSRYGSFSEIGYALAGTTNRYTYRSPVAGGTGASAPVAY
                     PAANYDQIVCGAPAPCVAAGDNATFPSNASTVAGSVGFTATAVASIDNDATIDSWHVN
                     DIKAGLTAADIDDVVS"
     gene            complement(32735..33262)
                     /locus_tag="PK224_10630"
     CDS             complement(32735..33262)
                     /locus_tag="PK224_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRB16236.1"
                     /translation="MVTTSKQQGFTLIELMVVVVIVGILAAFAIPNFLRYRAQAMQAE
                     ARSNLAGIFVAETSFFSERKEYGNFTDVGFAIAEGGTNRYTYRSGLGIGSGMGPNGGN
                     LCGSSGSCDTIQTASPMAGAMTYTGAVGSATTSASGFTATAAADLDGDATHDGWYVND
                     AKQGLNGAEPNDVSS"
     gene            complement(33658..35343)
                     /locus_tag="PK224_10635"
     CDS             complement(33658..35343)
                     /locus_tag="PK224_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16237.1"
                     /translation="MPSSSDSRASYHFLLGYQAELDQETELAIKEYQLALQTDPASNY
                     LKARLAVLYFTAGDVPSAVRFADEVADVPGLDAQMLGQIGGMYAAAGKPDKALRLFNR
                     AIEQEPQRSEHFFAKGLLQANQKQYVEAEDTIRAGIKISPDSAVGYYYLGRIGVEARD
                     FDKATTHFEQAVALNPAFEPAYVALGSVYEAKQDRDKAIGIYRRYLQGVNPKNRDIRH
                     HLIRLQVSAKQYDEALRELQEMLSEDPSDLDAQLRMGLVYGEQKDYPKAIQQLKQILT
                     VRPTELKVRDYLGYLYEETKDYANAMEAYRYNLTLEPSYFEGHLHLGVLQYRTKQYAE
                     SIQHLREASRLNPKQPEAHIVLGLSHFQVEQYEPSLQAFQEGIRHNPDNADLHFNAGT
                     AYDKLNRFDEVVKSMETTLALDPHHADAMNYLGYSYAERGVKIEEAIALTKQAVALRP
                     TNGYYVDSLAWAFFKKGLLAEALAEMKRAVALVGDDPVIYEHLGEIYLKQQHLSDGRE
                     ALLHSLELDPSNDKLMQRFRELGLGDPAKEERIRQALRRVSDRKGATPAPAQQ"
     gene            complement(35603..36958)
                     /gene="dnaB"
                     /locus_tag="PK224_10640"
     CDS             complement(35603..36958)
                     /gene="dnaB"
                     /locus_tag="PK224_10640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRB16238.1"
                     /translation="MKSLSAVDLTSPKLPPQNVEAEQSVLGAILLDNTAMAKVMELLT
                     DEEFYRTAHRKIYQAMLELSDRGEVIDQITLTECLKARSELEAVGGSAYLAELVQVVP
                     TAANVRYHSKIVRDKALLRGLIQTSTEVVTRGYDGTVAVDELLDFAERSVFSLAQGKL
                     DRSFTPVNQIIKESLDVVDKLSKRKERVTGVPTGFIDLDDLTAGLQPSDLIVIAGRPS
                     MGKTSLALGMAQHAALHAGTVVGIFSLEMSKPQLVLRMLSSEARVDSHALRTGRLQKE
                     DWWRLAEAAGKLEQAPIYIDDSGAVTVQQMRGKARRLKAERGLDLLIVDYLQLMQGKS
                     DSESRQQEISDISRSLKTLAKELNVPVIALSQLSRAVEARKPPVPMLADLRESGAIEQ
                     DADVVMFIYREEVYEPATERKGIAEILVSKHRNGPIGKRDLFFHDRFAKFENLESREV
                     V"
     gene            complement(36955..38079)
                     /gene="hisZ"
                     /locus_tag="PK224_10645"
     CDS             complement(36955..38079)
                     /gene="hisZ"
                     /locus_tag="PK224_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRB16239.1"
                     /translation="MGALSLKASSSSSPLVAGRERSLIPVGMSTILPDAARRIRQLEQ
                     TLLAVLARAGYEEIILPMFEYLDVLAPGLESELIEKCYQLVDRTTGRLMLLRPDATAQ
                     IARTVAMGMMGERLPLRLCYRTSVFRYEREHAGRDREIFQVGAELIGVDGVTGDAEVL
                     TLLLECLAQVGLSSFKVAVGHVGFFTALLVKAGLSQDGQKRVEQAAARKDMPLLEELL
                     TREGVSRSTGRVILEALELCGGPEVLAHGRKLVGRDRTLLRPLDRLAQVYERLVSPGQ
                     QAVLLDLGEFRGFEYYDGIVFDVFAPGIGAELGGGGRYDHLMGRFGRTAASTGFGLDV
                     DRLFRALDASGVVTPPHSSPSTTAGSKPVAAEPRRRRSRV"
     gene            complement(38098..39690)
                     /gene="serA"
                     /locus_tag="PK224_10650"
     CDS             complement(38098..39690)
                     /gene="serA"
                     /locus_tag="PK224_10650"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRB16240.1"
                     /translation="MKILVSDSLSKQGVEVLEKAGFTVVVKTKLPKEELLKEIKDADG
                     LIVRSGTKVTAEVIAAASQLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNTVTTAE
                     HTMAMIFSMSRRIPQATASTKGGKWEKEKFMGVELYNKVLGIVGVGQIGGYLTKLAQG
                     VGMQVMAYDPYLAPERAEKMGVTIVELDELFRRADVISVHTPLTPETKALINAQAIAK
                     MKTGVMIANCARGGIVHEGDLCEALKSKKVAAAAFDVFEDEPVKPDNPLLALDNFICS
                     PHIGASTTEAQENVAIGIAEQIVEYFTKGIARGAVNIPSVSPELLPKLQPYLSLGERV
                     GLLQAQLLEGGLERLTVEYSGEVAGLNVAPLTIAVLKGLLTPILEDPVNYVNAPVVAK
                     ERGIEVKEVKISDAGDFASVIRVRVEAGKKSHQVAGTLYHRKDPRIIEIDQFKVEVVP
                     DNHLLLIQNEDRPGVIGTVGHILGDHNINIARMQCSREERGGKALQIFGLDAPLPKSV
                     LDQITNSKHILSVKVADLSKGL"
     gene            complement(39719..40864)
                     /locus_tag="PK224_10655"
     CDS             complement(39719..40864)
                     /locus_tag="PK224_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRB16241.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDKLFAEVRED
                     LKWLFQTRNDVLILASSGTGGMEGSVSNFLSPGDKALTINGGKFGERWTKLCKTFGAQ
                     VTELKVEWGRAVDPQAVADALKKDPSIKAVYVQASETSTAVAHDVKALADIVKGYEET
                     ILVVDAITALGVLDLKTDAWGLDVVITGSQKALMLPPGLAFASVSEKAWRLADKAKNA
                     AFYFNFKRERENQQKSTTAYTPAVSLILGLKEVMNMLKAEGLEAVFARHAMLATAMRE
                     GVKAAGLSIFPQERPSDALTAISAPEGVDGQAVYKNLRTQYGMTAAGGQDHLKGKIFR
                     ISHMGYIDSFDVITALAAVEMVIKGLGYPIKLGSGVAKAQEIIMGKS"
     gene            41170..42561
                     /locus_tag="PK224_10660"
     CDS             41170..42561
                     /locus_tag="PK224_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRB16242.1"
                     /translation="MHARFLGTGVRALPVLSFCLVSLAWPALAGAESPTPSHADTPAT
                     TETTPEDIQPSIQEPEYSLDEEDHATAAELEAVEPPAPIASEPSTEVPPAEALLQLKP
                     LNGTTARFTDLLTPPAEVVQEEASAQDQESDEATAYNVPIVLDPSVQGHIRFFNVSIR
                     NRFEQWLIRLSHYRPLVDSIFSEFQLPSDLIYLSLVESGFNPHAYSRARAAGPWQFMK
                     GTAKVYGLRVDSYVDERRDPVKSTVAAARYLRDLYDLFGTWPLAMAAYNAGEGKVMRA
                     LHTAQAESFSDIAKTRLIRRETKEYVPRFMAATIIAKNPDRYGFPQNDVRPHQFEEVV
                     VKRPIHFKAIANVTGISYQELKVLNPELRRDATPPDDAEYHLKVPVGTREKVEQLLDR
                     APTHKFAPLPVPVRVRHVKPEPESGHWYRVRVGDSLERIAKRFNISVKTLKSNNNLTG
                     PTIKVGSRLVIAN"
     gene            complement(42602..43435)
                     /locus_tag="PK224_10665"
     CDS             complement(42602..43435)
                     /locus_tag="PK224_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16243.1"
                     /translation="MSYRIKVPAKVDPLDEAHLLTGVERFLLVLQEQRRAVLVGLGVL
                     LVAAAVVAGVIWYDYQATLKARELDQEATLHYLNRPADDPKKSHEQLAQAINLYKQVV
                     DQYPRSPVAPLALFHLGNAQVLANEVDAGIETYKRFMLLHGSNTSLLGLVQQRMAYAY
                     LVKGDRDQAVKAFTGILEIPGALNKDHVLFELAKLEESQSRPEGALAHYQDLMKNYPN
                     SPFTSEAAVRVKVLEVKKSPESPAAAATPAPAVTAPAPQPEAPAKSSGKSAAASSKKK
                     P"
     gene            43557..44717
                     /gene="bioF"
                     /locus_tag="PK224_10670"
     CDS             43557..44717
                     /gene="bioF"
                     /locus_tag="PK224_10670"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRB16244.1"
                     /translation="MFKEYLQKLQDQHLLRRLRTIASSTGPTVRLDGHTVILLSSNNY
                     LGLATHPAVVEAAVEATRQYGAGSGASRLVCGTLTPHEELETALARFKDTEATLTFAA
                     GYLANISAVPALIGKDGLILADRLCHASLIDGCRLSGATFRIYHHRDMNHLEQLLGRR
                     PPGKPTLIVTDGIFSMDGDIAPMRDIALLAEQYGAAVYVDDAHGTGILGETGRGTLEH
                     CDVETRIPYHMGTLSKAIGSSGGYLTGSAEFVAYLVNTCRAFTYTTAPTPAAAAAATA
                     ALRVIRREPERRARLWRNRHRLAQGLSGLGFRLAASESPILPVIVGDPDRAMNLAHTL
                     LTLGVYAPAIRPPTVPASTSRIRFTITADHTDEHIDQALTALAQAGRTLNMI"
     gene            44896..45957
                     /locus_tag="PK224_10675"
     CDS             44896..45957
                     /locus_tag="PK224_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRB16245.1"
                     /translation="MIDISKLLTFGVQQGASDCHISAGEPPMIRLHGDLKKLDHPPLT
                     QDETHALIYDMMSDAQRKNFEEHRECDFSFDLGDIARFRVNVFVQGRGLGAVFRTIPT
                     EILPLEKLGMPPILRQLCDREKGLILVTGPTGSGKSTTLAGMIDYLNNTFEGHILTIE
                     DPVEFVHKSKKCLVNQRELGVHTLSFANALRAALREDPDIILVGEMRDLDTIQLALTA
                     AETGHLVFATLHTSSAPKTIDRIIDAFPPNQQAQVRAQLSETLEAVLTQTLLKKKSGG
                     RIAAVEIMVGTTAVRNLIREGKLHQIPGIMQASQKDGMQTMDMALVDLVTRGLVTKAE
                     AQSRSMNPNLFSAAAGGAA"
     gene            46014..47177
                     /locus_tag="PK224_10680"
     CDS             46014..47177
                     /locus_tag="PK224_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRB16246.1"
                     /translation="MDVRTLLEVMVKQESSDLYLTVDAPPIYRIHGSTHRTDAPPFTN
                     EQLESLALALMRGQQRGEFEEKMEMNLALYYKDLGRFRVNIFRQKGNVGLVFRHIKAE
                     IMTVEQLDLPPIVKDIAMTKRGLVLVVGATGSGKSTSLAAMIDHRNTVHAGHIISVED
                     PIEFVHHHKKSIVTQREVGFDTHSFGHALKNTLRQAPDVILIGEIRDTETMEAAITFA
                     ETGHLCLGTLHSNNANQSIERIMNFFPVERHAQIYLQLSLNLRAIISQRLIPSLDGRR
                     VPALEIMLDTPRIKDLIKRSEIDTLKEAMEQGIDEGCQTFDHVLLQLYKAGKISIEQA
                     LINADSANNLRLKIKLAGLKGDDAVAALLDKSDRDEKGFQIQGQLGQGGSKKR"
     gene            complement(47259..47780)
                     /locus_tag="PK224_10685"
     CDS             complement(47259..47780)
                     /locus_tag="PK224_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16247.1"
                     /translation="MLDSGAASSVPPPNRKHKAVATFAAGLVFLVALAGIRNISPPSD
                     STPAVQNIDPNMVPLVTGDEPIQELFVRAGCPVCHQIPGIAGAKGQVGPPLWLGKTGA
                     SRLADPQYKGQAQTVREYIVESVVSPGTYVVPGFPPDTMPTWYGRKLSGAALSKIASY
                     LEQAVEEPPSGRP"
     gene            complement(47829..48686)
                     /locus_tag="PK224_10690"
     CDS             complement(47829..48686)
                     /locus_tag="PK224_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB16248.1"
                     /translation="MRLFSMTFVVSVILFVGVWSVRAAEPLSGADGQAGGAEAITGQD
                     GAPMVLVPAGPFTMGSNDGLPAERPEHVVTLDAYVIDRYEVSLQLYRKFLQAARYDAP
                     PTWDDEAAETVSDRPAVGMSWEDAAAYCAWAGKRLPTEAEWEKAARGTDGRRYPWGPM
                     QPFVDIANYNRGVWVSEAITLVSVAGGVEGMSVRHGLKAGGRSPYGLHHMAGNAAEWI
                     ADWYDRTYYGKSPDKNPTGPANGEKRVIRGGSWADLPVALSASARVSAEPGFQDRTIG
                     FRCAMDAKK"
     gene            complement(48953..49447)
                     /locus_tag="PK224_10695"
     CDS             complement(48953..49447)
                     /locus_tag="PK224_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16249.1"
                     /translation="MMSHRTVISFALLAVLVGGIHGCTTMQAGSSNTLPSSAFFPVDA
                     NELKSLQAIAHAQDSRMKNCHKGSACEDAYYTRGLVALFENRADAITVFQELHTAMPN
                     SRYEAATTGWLNLLQDTAPSSKHHQALMVQLKQEVLHNLLERSDLTALRTVKDHDRRV
                     AELK"
BASE COUNT        11379 a        15277 c        12904 g        10088 t
ORIGIN      
        1 gtgacccatg gagtcgaaca acggggcgcc gacggactga ccccgtcgga actcctggct
       61 cgcatcgccg cgctgacgca ccagctggtt catgagcaat acgtcgtcct gaatcaatca
      121 ctcatccctc agctggccga ccaacgtatc cggtttctca aacgcgccga ttggatgccg
      181 tcgcacattc ggtggatgcg ccggtttttt tcgcgcgaac tgctcccgct cctgagtccg
      241 gttggcctgg atcccgcgca tccttttccc aaactgctca acaagagtct caactttctg
      301 gtgacgctcg aagggaccga tgccttcggc cgccccaacg gaaccgcgat cgtgcaggtc
      361 ccgcggtcgc tcccgcgcgt gattcacctg ccggtgcgca acgccgcctg gccccacgat
      421 tttgcgttct tgtcgtcggt gatccatgcc tttgtccatc aactcttccc cggcatgcac
      481 gtcaccggtt gttaccaatt ccgtgtcaca cgaaacagca atcttttcgt ggacgaggaa
      541 gacgtggacg acctccggcg ggcgctcgaa ggtcagcttc ccgaacggcg attcggcgac
      601 gaagtgcggc ttgaagtggc ggacaactgc ccgcccgacc tagtctattt tctgcgggaa
      661 cagtttcatc tggatgcgcg cgatgtgtat cagtgccacg gaccggtcaa tctgcatcga
      721 ctgatggccg tgcccgacct ggtggatcga cccgatctga aatttcagtc ctttaccccc
      781 ggcattccca cgacgcctgt cccgagcgaa gactggttcg acgccatcag gcagggcgac
      841 atcctactgc atcatcccta tcagtccttt gcgccagtga ccgagttcct ccgccaggcg
      901 gccacggacc cgcacgtcct caccatcaag caaaccctgt atcgcacagg ggcggattca
      961 gccatcgtgc aatccctggt ggatgcggcg cgaggcggaa aagaagtgac cgtggtgatc
     1021 gaactacggg cccgtttcga cgaagaggcg aacatcgagc tggcgcatga tctggaagag
     1081 gccggcgccc atgtggtgta cggcgtggtc ggacacaaaa cccacgccaa aatgagtctc
     1141 gtactccggc gggaaggccg gatgctgcgg agctataccc acctcggaac cggcaactat
     1201 cacgcccgca atgcgcggct ctataccgat tttggattgc tcacctgcga tgcggcaatc
     1261 ggacgggacg tccgcacggt gtttcagcaa ctcacctccc tgggacggcc gggacggctc
     1321 aaacatcttc tccaatcgcc ctttaccctg catgccacat tgctgaaatg gatcggccgt
     1381 gaaaccgaca gggctaagca gggccgacca gcccacatca tcgcgaagat gaatgcgctc
     1441 ctggagcctc aaatcattcg ggccctctac aaggcctctc aggccggggt gaaaatcgac
     1501 ttgatcgtcc gaggtccctg cgccttacga cccggcatcg cgggcctctc ggaacatatc
     1561 cgcgtacgtt cgatcatcgg acggtttctc gaacatagcc ggatcttcta ttttctgaac
     1621 gacggcgacg accgggtctt tctctccagc gccgattgga tggatcgcaa tttctttcga
     1681 cgcatcgaag tggcgtttcc tgtgctggat aaaacattgc ggcaacgcgt gatcgacgag
     1741 ggcctccgcc cctacctcga agacaatacg cacgcctgga ttctccatcg ggatggcacc
     1801 taccggcgtc aaacaccagg acgcagagcc gctcgttcgt cccagcaatg gctcatgaac
     1861 aagctcgtca cctaacctcc tggtaggcgc aaccactcgt cattattaac ccctccttaa
     1921 ccggttgctg atccggttgt cacgtcagcc ctctatgctg cgggctcgac aacgaggagg
     1981 ggagtaccat gagcgtcgcg cagggcattc gactccatcc acaccaccaa gcattcgcgt
     2041 cgttacgcac acacctgctt cgaagccgcc gcatgctcga cacttcgtac ggcatctgcc
     2101 atcgctgccg agcggacatc cctctgccgc gactacgcgc acaacctgat gcgacgctct
     2161 gtttcacatg caagcaattg tctgaagaac gggcttcggt acgacgcgcg gcatggaggc
     2221 ccgacacctc cgcagaagcg gtttaccgat cctgaaagcc ggctaccgat tgggagttga
     2281 gctgcgccac ctctccgcac ctctctcccg ggctgttccc ctcttctgag ccggagcgga
     2341 acagcccacc attttgaccc gaaaccgcgc tcataggggg ccgatccccc ggccccttac
     2401 agcccagccc gatcacacca tcgttgtaag ggagaaacaa tcaggaaaag aaagattgga
     2461 atgacccggg agcggtgatt ggccacgcga gattccgtgg cgattgccgc atgcaatccg
     2521 caacctccag tgtccgcggc acaatcggca cgaaccagct cagaagcctc ggcatgaagg
     2581 cctgacgctt cctacccccg gcacgatgcc tctagcaggc tacctgacgg cacgcaacga
     2641 tccagcgtat accggaacag gtacaagccg atattcagac gagcggttgc cctcgcgact
     2701 agcccggact attcatgcat gccgtcgcga ggcgttcgag accgaagtcc cgcggggctt
     2761 ctcgcacgtg aggccgacgc aggcgtactt gcagtccgtc gaggaggccg aacgagaaca
     2821 gccccgccgg gcgagaggat cggacgacgc agtaggtatt catgaatagt ccgggctaga
     2881 gcgcatccgc agacaaacag tcgagacgtt cttgaacgca gcttgtgcac agagtcgcgt
     2941 gtggccggtg tctcagttgt atgaagggga tctccttccg gcactccagg cacagaccag
     3001 tgatcctcag ccgtgtatgc cggagagaca aaatatcctg gcggcgcggg cgagaccgaa
     3061 acatctgaat gccttgcttt acgagtcgca tgctacacct cctgtgctga gagcaacctg
     3121 gtcaccatca gcctacagac ccagaggcac aggcacgtcc ggacaaggta aatttttcgt
     3181 taacaggtga gggtcgctgg agcagagagt tgtattccgc tggaaggcgc ccctgcgttc
     3241 tgtgcgcagg ggcgtcgatt cagaccatca cacgatcggg gcacacaacg gagcggcgct
     3301 gcatcggatg catacctaac cccgccctac ttcaccgcag ttcgacgccg ttcgatcacc
     3361 ggcgcccaag ccggttcaag gtcgcgcttg gcggcagcca ggaaccgctc ttcggcagcg
     3421 acgtcccgac tcccctgttt gacgctgacc gtatgcacgg cgaaataggc cggcgtgccg
     3481 atcatctgga gatcggctat gacctgggcc agcggtttgc cccaaaatgc ttcgaaataa
     3541 tgctcctgca ccggatcggc cgccttctcc tgcaggacca tcgtggacgt gccgacttcg
     3601 tactccccca tgaaactgat gctgccggaa actacggtcg tatcgctttg cttcacgacc
     3661 gtcttgggta ataggaatag agtgacttcg cgtaccttgc cgaatccctt ctccgaggtg
     3721 ctgaatgcca cggccgcata ccgcccgggc gggacatttt gcacatagac cgtcccgcct
     3781 cccccggccc cgggaggcgg agccagaatg ctggttggaa tcagcttgga accttcgaag
     3841 agatcacgct cgttctcgac cttcaccaaa tacaaccgat cttcggtgtt gggtacccag
     3901 gccggactct tgagcgtgat cccgaggacc gcactctgtt gatcgaccgg cttcgtgatg
     3961 tgaaccggcg agacgaattc acccgccagg cgctgaaact gtttcttcgg aggcttactg
     4021 gcacaggccc cggtggtcag aacgaccagg gtcagcagcg caatcaccgt tcgattcatg
     4081 gaggtccctc tgggtggatt aactgagcag ttcgcggcca cagtgtaggg gattaggcgt
     4141 actgcctcaa gcgatacttc cggcgggcat ctgccttgaa ctgactccat gcgagagctg
     4201 ggcgatagat cgagtacagg gcgtacggtc agggggtaat aacggagcag aaatttacag
     4261 caatgggaag gtaggaatgg gcagcacaat ggctacctta tggcaacggc gaaaccggag
     4321 caagtggaac ctccgcagca gttttccctt ggctctgact ctccagtgag agacgcttca
     4381 agaccgcctt ggctcgctcc tccagacgct cggcttcgcc ggaccgctgc cgacgcctca
     4441 acagggacgc ataactcttc aaggccttca cgtaggcctc gcgatcatac ccgacggact
     4501 gttcgtagat gaccagcgac tgtttataga gatcctcgga ctgttccaac ttattctgcg
     4561 attgatagag tcccgccaga ttcttgagtt cacccgcaac gtgagggctc tctgcgccct
     4621 gctgcttttc ccggattgca atcgcctgct gaaagagcgg ctccgcctgg gagtagagtc
     4681 cctgcaactc atagagttgt cccaagcgac tcatcgcctc ggcggtatcg acatggtcgt
     4741 tgccgagaga ctttttaaaa atgctgactg cgcgttgata cagcacttcg gcttgagggt
     4801 attgattttg ggcccggtac gtatctgcaa gattctcaag attcctggcc acgatcggat
     4861 ggttgggacc gaaggccttt tcatgaatcg ttaaggcgcg ctgatacaac ggcatggcgc
     4921 gagcatgctg cccctgcgcc tgatacatct tcgccaggtt gtcgcgcgtg atggccgtcg
     4981 tgctgtgttc cggcccgagc tgtttttcaa gaatcccgag tgcgcgctgc aagacaggct
     5041 cggcgtcggc gaagcgtcgt tgatcctgat agaccacagc aatattgttc aaactctcgg
     5101 ctgtcttgct atggtccgcg ccgaacacct gctcgcggat ttcccgtgcc cgttgatgaa
     5161 gcgcttcgga ctgcgtgtag agtccctgta ggcggtagag ttcgcccaag tcggtcaggc
     5221 tggcagccgt ctcggcatgg gccggcccat atacgcgctc cctgatcgcc agggcctcct
     5281 gaaagagtgc ctcggcgcgt ggaaaccgcc ccaactcacg attcacttcg cccagctgac
     5341 tcaacgtctc ggccaaacgt ttcccttgtg gatcgagcac ctccgcttcc ttgcgagcag
     5401 ccacaaagga ttcctccgca tgcgtgtagt cgccggaacg cagggcgtga ttaccttcct
     5461 gcatggctga acgccagcgc tggtctaccg cgcatcctga gacgacgccc agaaaggcca
     5521 gaccgatggt aagcgcacgc gcattcaatc caatgttcat gatgatcaac tcttcggtgg
     5581 tgagacggtt gccgtttggg gactggcctt gctcgtagaa tactcctaag acttaccccc
     5641 tctgacgcct caaatcaacc ccacgagggc aactttttcg atcccttcgg catcgctata
     5701 tttttcaacc acttccccta atgcacacat ttcgaccaca gaggatgaaa cggccccatg
     5761 ccgatgatta acaaggaacc ggaccatgcg ggagagacag ttatcgtttc tcggaggcgt
     5821 gatcgacctg aggggcaagc ttcgcaatcc cagaggtatc gtaggggagg acgctgaaca
     5881 ccttggcgac atcgatagcc ggcttcccgg gacgcaggat tttctgcgcg agcgtcgcca
     5941 gccaggagag gggaactaac acaaacctcg ggagccagac caccgtgaga tcggggttgg
     6001 cttgccggag acgatccaac agttcctgct tggtcgggga aacaggatcg aggaggttca
     6061 aggggctcgg cacgttgtcc cacgcgtccg tcatccagcc aaggaagcgg ccggcaaatc
     6121 cgacatctac cacccccaga cgatcgcccg gcgaaccgac agccacgaag atattcccca
     6181 accgcttacc aaggcggcca ggcgggtcga aatcgcgata gtcgaccaat gccccgggac
     6241 gaatcacttt cacggataga ccgagttctt tgccgagttg gaccgccaac cgttcggatt
     6301 ccaactttcc ccaaacatag ggaccggacc ccttgctgtc cggctccaac ggatgatcgt
     6361 cgccgatggg ccggccgttg ccctgcgcca atacggcaag actgctgaca tggacgaaat
     6421 gcgtaatccc cgccgcatcg gccccgcgaa ccatctgctc ggtggcatcc aacgagttac
     6481 gctgatgctc cggccagcca ccggcggttt ctgccgcagc atgaatgacg gtatccaccc
     6541 ccttgaagag ctgtgcggcg gctcccgtgg ccacatcggc caccacatat tcggcgccgg
     6601 caatccgctc ccagggggac ggctcacgtc gcgccaccac acgaacaggg cggcctcgcg
     6661 acagcagcgt tcgcacgatt tcttttccca aaaacccggt tccaccggtg accagcacgc
     6721 cccggctctc gaccggcttc ggcgcggcgg cagccagggc cttggcctcg ccgaccttca
     6781 gggccttcgc cacgcgctca cagatgcgca ccgtctccag taaactctcc ggcgacaagg
     6841 gcgacggccc gcccgtccgc gcggactcat agaacgcggt aaacagttcg gccagcccgg
     6901 gataactccg ctgactcttg agaaatcggt tcgccatcgc ggaggtcgtg ccgatcagca
     6961 attgccaggc ctgccggtaa ggcgcgaata acttatcgat gccggacgac ccgggaccga
     7021 tggcgcgctg agtggtactg cgaacatagt cggcgaacaa cgacccgttc ttcccgacga
     7081 cccgcagata actctccacc gggcgccctt cgagcgtcac gatcaacgtg ccggtcacgc
     7141 cgccgcgtcg caccaacgca tgcaccgtcc cggcctgact cacttccaag gacaacagct
     7201 cggtacggcc ctccccggct tgctccaaca cctggagcaa cagataaacc ggatgcggca
     7261 gaatatcgag taactggtgg tcggcgcgta gcaccttgcg gccacccggc gcatgacgca
     7321 cggtccggaa ggaaaagtag ctctcgacgt gcacgacgcg cccaattgac ggcaagtact
     7381 gggttaatac ccgggtgggc ggttcataca acagctgatg gccggcacag acacgaagac
     7441 cttttgcgct cgcctcatcc aggatctgct gggcgtcctc caccgactcg gtgaagggct
     7501 tctctacata gatgtggcat ccggccttga tcgccatcct ggccaacgga gcatgggagg
     7561 ccggcggagt aatgatgtgc accacattga gacgttcgga ggcaaacagc tcctccggcg
     7621 tcttgaaaca gccgatgcct ggaacaatat cgcgcatcgc cgcttgagcc gcgtcggacg
     7681 ggtcggccac cgccaccagt tgcacgccgg ggcagcgcaa aatagcgcgg gcatggtgct
     7741 gggcatgacg gccggcaccg atgagcgcga tccgtaacgg gcgcgagtct ggagttgtca
     7801 gagtagagtt ggccacaagt cctgtgtgag tgtgcagcgc tactatagtc tattctcact
     7861 cattcttgaa gccttctcga aaattagcgc agggaggaag actgcggcgg tgggcgcaac
     7921 gtgcgaggga cctcgtgtga acaggtccac acacatctcg aactacttat gcctttcgtc
     7981 gcagggaaaa cattgatcca tacattccgg gcagagcccg ccgttgaact gtatcgccga
     8041 tcgctcggcg ataaacgtgg ccaggtcata ccattcgccg gactcgtccg gcacacgctt
     8101 gcaccaggaa cacaaattga taattccctg cggacgtccg agctgcgcat ccatcatcca
     8161 gaagccctca cgctgtccgc cctggacctg aagcccctgc ccgcttgagg cgatctcatg
     8221 gaatacaagc acggcgccga gcacgatgcc gccgtcgtcg accaccggcg ccacacttcc
     8281 tcgaatcgtc cggcacgcac ccagcctgga gatgagggtc gctccgtcga tcgtcaccat
     8341 gcagacctgt gccatcgcct gctgagccgg actttccgat ggtggcaccg cttcggagcc
     8401 caggagagcg ctcacagaca tccccgtcgc ttcctcctga ctccaaccag tgaactcctc
     8461 tgcggcggga ttcatatacg tgacccgccc gccacgatca gtcgtcacaa tgccgtcacc
     8521 gatacagcgc accgtggtgg cgatccagcg taaccgctcc cgcatatggc gatcgtgctg
     8581 cgcgcgatgc agcgccagct caatcgtggt ccggagttcg ttcgcttgat agggcttgag
     8641 catatacccg gccggagacg tgaccttcgc gcgctccagc gtgtggttgt cggcataggc
     8701 cgtgaggtag atgaccggca catcctgttt tcgctgaatc tggagggcgg tttccactcc
     8761 gtccattttc cccttcaata caatatccat caaaatcagg tcaggatgca tgtcgatcgc
     8821 cttgcggatc gcctcttcgc cggaggtcgc cgtagccggc acgcgatacc ccagccgttg
     8881 caggctcagc tgaatgtcct tcgccacaac gggttcatct tccacgatca agatgctggc
     8941 tggttccatg gcgttcacac cctttctgaa tattgcagtt gctgaaagcg aatctgccat
     9001 tccgtgcccg ctccactccg gagttccgtg ctcccgtcca gcttctctgc cagcagcgtc
     9061 acaagttcca gccccagtga atcgggattg ttcagcacgc cgtccttggg aatcccgatg
     9121 ccgttgtcgc tcacgcacag ggtaaacgtg ccgtcgacat gatcgagcag atcgatgtgc
     9181 accgttccac cgctgtcgtc gataaacgcg tgcttcaaac aattggagac cagctcatcg
     9241 atgatcaacc cgcaggtcag ccccgtgtcg atatcgaact cgacatcgtc cacgttcagc
     9301 tccagcgaaa tgaggttcgg atcgaccccg tatgaacgaa acagatggcc ggtcagggtg
     9361 cggatatagt cgcccatttt gattcgcgaa agatcgtgcg accggtgcaa cgtttcgtga
     9421 agcagcgcga tcgaagtgat ccgcacctga cattctcgga acagctggtt gacgaccggg
     9481 tccttgatgg aggccgattg taaattcaac aggctggaaa tgacctgcag gttgttcttg
     9541 acccgatggt gcacttcccg cagcagactt tctttttcct tcaacatccg gcgtagctgg
     9601 tcttccatgt ccttgcgctc ggtcaggtcc atgcaggtac cgatgtaccc gccgaactgt
     9661 ccctcctcgt cgaacaaggg cactcccgta tccatgatcc accggtactc gccgtcgtga
     9721 cgccggagcc ggtattccag aaagaacggc tgctccgact tgaacgcctg cagatacgac
     9781 ttccggcagc gatccaggtc gtcggcgtga atgcctgtaa accagttgtc gccgatctct
     9841 tcctgcacag tgcgcccggt aaattccagc cagcgtttat tgatgaaggt gatgtgcgtg
     9901 tccggtccgg ccatccacac catcaccggc gctgtgtccg ccatcatgcg aaaccgggcc
     9961 tcgctttccc gcaaagccgc ttcgatgcgc cgcttcaccg tgacatcgcg cgcgatagcc
    10021 gacgcgccca tgacacaccc atccgcatct ttcaccggcg agatcgtcaa cgacacatcg
    10081 atgcgctccc cctgtttccg gcgctgcacc atctccacat tgcgcacgtg ctctccccgg
    10141 gcgatccggt cgagcatcgt cggcacttca tccaatcgat tcgggggaca cagcacagag
    10201 atgggtctgc cgagcacctc ctcggcgcga tacccgtaga cccgttccgc cccccgattc
    10261 caactttgaa tcatgccgtt caacgtcatg cccacaatgg catcgtccga tgatttgacg
    10321 atcgcgacca gttcagacac cgcttccgct cgcagcactt caagttcctg attggcggca
    10381 gccagatccg ccgtgcgctc ctgcacctgt tgctcgatat cttcattgat acgcaacaat
    10441 tcggcctcgt cgcgctgccg ccgcaaacac aacagcgcgg tcacccaaat caccatgagc
    10501 gcaaaggccc gattcatggc gctgacccag gtcaatgcaa agagcggccg atcgaacata
    10561 tcgagaatcg tcaccagaga cgcgagggcg gccacccaga acgtgagacg ggggtgaggg
    10621 atccgggagg cgatgagaac cggtactacg tagagcgtgg tgatggtcaa tcccaggggc
    10681 aaccaccagt caatcgcgcc gatcatcgca atcaacgcca gggccactcc cagcctggag
    10741 tagctgctcc gcgacatgcc gagaaagaat gatgcaggta ttgtcatacg tacaacgacg
    10801 cggcgtctcc ctcatcatcc atatgagcaa tccctatgcc tccggcgcca catccacccg
    10861 ccaatcgcac ggggaaacag acctcacttg gcaaccaaga tctggtgata ggcggccaag
    10921 ccttgaccgg tgccgcttgc caggattgtc caggctgcga ccggttctca tattgagccc
    10981 aacgtcggcg tcgtaggaat tccattcagc gcaagcccat gccccacagc gggccctggt
    11041 gtacgtcacc gcctcatggt tgttcagact tgaacgccag gctacgggag cgcttgtcgt
    11101 cgattcgatg accggaaggg aggcacacat gcggtcagtc aagccggagg aggcgccgtc
    11161 ggcggcggcg tcacaacgtg caacaggcaa cgaccgtcat agggcgacac gccaggagca
    11221 ctgcttctcc ggcggaggac cgtgacactg tcggctatca ggatgtgaaa aagaaccgtc
    11281 cggcccggaa ggaaaccggc gccggacggc aacgcgaatg ttagcgtttg cggtagaacg
    11341 aggcgatcgc ctgcacgacg tattgaagct gttcgtcgga aagttcagcg tagatgggaa
    11401 gcgagagcac ttcttcggcc gcacgttccg attgcgggaa cgcgcccttc tgatacccca
    11461 gatcacgata acaattttgc aaatgcaggg gcaggggata atagacctct gaacccacgc
    11521 cctggtcttt cagataactc cgcaactcgt cacgcttctg tactcgaatc gtgaattgat
    11581 taaagacgtg aaagttgttg gcccccacga cgggcaaggc gatccggtct gtcagtttcg
    11641 cctccgtgaa caactgcaca tagcgcgcag cattccggcg acggccttcc gcccactcat
    11701 ccagacgttt cagcttcacc aacaagaccg ccgcttgcaa cgcatccaat cggctgttga
    11761 tgccgatcgc ttcgtgcaca tagcgcaccc ggctcccatg aacccgcaac atcgcgatgg
    11821 actccgacag cgccttgtcg ttggtggtca ccatgccggc gtccccgaac cctccgaggt
    11881 tctttgacgg gaaaaaactg aagcaggcca catcgcccaa cacaccggca tgccgtccat
    11941 tctgacgggc accgatggcc tggcaggcat cctcgatcac gccgatcttg tgtcgtcggg
    12001 cgatctcatt gatcgcatcc atctcggcgc attgaccgaa gaggtgcacg ggaatgatcg
    12061 ccttggtctg tttcgtaatg gcccgctcca acagcgccgg atccatattg aaatcatcag
    12121 ggcgaatatc gatgaacacc ggcttggccc ccagcctgga aatcgccccc gccgtcgcaa
    12181 agaaggtaaa gggcaccgtg accacttcat cgccggcttt cacccccaag gccatcagcg
    12241 ccagcaacag cgcatcgctg ccggaggcga ctccgacgcc atgagcactg ccggtatacg
    12301 tcgcaaccgc ctgttccagc gccaccaccc gcgggccgag aataaagccc tgctcgtcac
    12361 aggtggcctc aatcgccgcc aggatttcac tgcgcatcga ttgatattgg gctttgagat
    12421 cgagcagcgg aatgttcatc gtatcccccg gttcccccct tttacagtcg attgggctgc
    12481 aacttcatga catcttcact cggacgcgtg gcatgcagaa gataggcata gagcgactgg
    12541 tactgtttga gcgtctcttg aatcgtaaac tcctccggga gttcctgcgt gccggcccga
    12601 aggtccgcac gaaaacgctc atcttccaaa atctgacaga cccgttcagc catggccctg
    12661 acgtcgccgg ccggacagag cgtaccttga atcccctcca cgatggcctc ggcagcgccg
    12721 cctactcgcg tggccacaat cgggatccgg ctggccctgg cttccaaaat ggcgcagggt
    12781 aacccttccc attgggacgt caggacgaac acatcgaagg ccttcagcaa cgcagccacg
    12841 tcgcggcgcc agccaagcag ggtcacacga tcctgaagcc cctgtgccag caacaacgct
    12901 tcgactttcg gccgcaaggc tccgtcgccg acgagcacac aacgagctgc gggaacacgc
    12961 tgacaaacga gcgcggcgac gcggacaaag tcttcgggag atttttgcgg tttcaagcac
    13021 gagaccgttc ccaccagcag ctgatcaggg cccactccca gcaaagcccg aagacggtca
    13081 cgctcctgcg gctcaatacg aaccgcaaag gctgtcggat cgattcccgg tctcaccacc
    13141 gacaccttgg acgcggtaaa gagcccccac tcaatgcctt gccggcggtc ggcgtgcgac
    13201 accgcaatcc agtgggtggt gatccggccc accatccatt ccagtgcgat caatgcttgt
    13261 tgttgccaga acggctgcac aggcgtcacg ccgtatccgt gtatcgtgtg caggatgcag
    13321 ggaacaccgg cgagccaggc agctaaccgt cccaggatac cagcttttga actgtgggtg
    13381 tgtacaatct ttggccggag tcgtcgaaat gcgccgacca gctcccacaa tgcgcatagg
    13441 tcttggaggg gacggatctc tcgaacgagc gacgggatcg tcccaacctc gacaccttcc
    13501 agcgcatgcg cgtcgtcggt ccgcagtccc cccggccctg cgagaagcac cggacgaaag
    13561 cgtctgcggt ccaaacccgc cacgacctgc atcgccactt cctgcgcgcc gcccaactcc
    13621 agcttcgtaa tcacgtgaca gactacctgc atgccattca cccgctcagg ccgcggcctt
    13681 catccgcaat cgagccgccg cctgttgccc ctgggcaatc gcgtcctcca tcgacgtgtg
    13741 ttcccacaat ccgtacctcc cgatcgacga gatcccgcgt cgctccaact cggccagcag
    13801 ctcttgcacc gcacggccac gataccggtc gaacaagaca tacgcgtaat agagatcctt
    13861 cacatccgac atcacgagtt catcggtccg ctgaagcact cccgcctgct ccaacccccg
    13921 ccggacacga tcgatgagta cagcgtcgga ttcccgctga gtcggctggt gcgagatttc
    13981 cacatagagg gaactgcacc cgggttgccc catggccgga gaaaaattca tcggaaaccc
    14041 ggcgcgatag aacggatacc ggtgctcagg aaaatagatc caatgtttgt cggaaatgcg
    14101 ctcacgggcg accgctagat tcacgttata cacagagacc catcgcagcg acgcagccaa
    14161 ttcccgcagc gaaggcggca gatccacaca acgccgcacc agatcaggca gggggatcgt
    14221 cgacacgatc cggtcatagg cttgcgtcca ttcaccctgt gcgctgcgaa agaccgctcg
    14281 ccgcctgccc gtctcgatct ctaccaactc cgaatcgtac gacaagttct cgaccgacgg
    14341 gagaaaggct tccggcaaga ccttgatgcc gccgctggcg ggatactgaa acgacggatt
    14401 gtagccgaag gccttgtcct tgattcccaa ggcgccattg acaacatcct tcacatccgg
    14461 cttgggcacc agccacgaca cccagtcgga cgtgagttca tccagcggca cctgccagag
    14521 cttctcgtta aagggcacca tgaaatgttt cgcaatcccc tcgcccaggc tctcgacgat
    14581 ccattgcttg aacgaacggc cctctaccgg aggcttggga gacggattcg acaaggtggc
    14641 gatgaatccc agcagacatt cccgcaccac ctccggcggg agaccatgcg tgttcacctg
    14701 aaacggatat tcggtatagg tgtcatgaga atagatgtag gatttacggg catgccgctg
    14761 aagttggtca ggcagcaggc tctcgaccag cgccttgatt gcggtctgcc ggaagtgaag
    14821 cagatgcccc gtatagtcga atgtgaatcc gtctttcacg tacgaacggc agagcccccc
    14881 gacctcgcgt tcccgctcca gaatcttgta gggcatcccg cgcaggtgat aggccgcact
    14941 gagtcctgcc aatccagccc cgacgattaa aatcatactg cgtcggtcct ttctgtcgaa
    15001 cgtaccgagg ccgcctcggc actcacggcg acggttccgg tggaaagatt cacacgcgtc
    15061 aaggccacag cagccgaggc caggcacagc attgtgacac cgcttaacac cacggcaaga
    15121 tcctgaggca tcgccatgac caggagaccg atccctccca acaacgcggc gccgagataa
    15181 ctgagcagga cgacttgggt caccgagagg ccccaatgcc gtaaccgaat cgcgagatga
    15241 tcagggctgc cgagaaacac cggcagcccg cgcaggaacc ggatgtacat gacgaacagg
    15301 gtatcgaaaa tgggcatgcc caggatcaac accggcgtca gcagcgagac ggaatggccg
    15361 ctcgtgtatt tgccgatcat ggacagggcg cccagcatca gaccgatgaa catcgcgcct
    15421 gaatcgccca tataaatcga agccggtcgc caattgtatc gaaggaatcc gagcagactc
    15481 cccatgagcg ccgccagcat gaatgcaatg gcctgatcgc cattcaagat cgccaccaca
    15541 cacagaaacg cggcactgat gacaccgaca ccggccgaca acccatccat gatgtccaat
    15601 aggttgaagg cattgatgat gccgatcatc cagaacaccg tcagcacgat atcgacccaa
    15661 tcgggcaacg acgcgatttc gatgcgaatg ccgctcttga tcaacacgaa caccgccagg
    15721 aactggcccg cgagtttggt tcccggcgac aacaccccga aatcgtcgat cagccccagc
    15781 atgacgatca gtgtcccgga gagcacgatg ccgagcacgt cctgccggaa ctcgaaggta
    15841 aaggccaggc tcacgagaaa cgcgagatag atcgccaatc cacctagata aggaaccggc
    15901 tcacgctgat gcttcagccg gccatccggg ttgtcgacga tgccgaattt cagggccgcg
    15961 cgacgcgcga tcggcacgcc gtagattgac agaagcatcg cgaccacaaa tgtcagcgtg
    16021 agcagaatca tcgagttcct gtcgtacggt gaccggacac agggctgtgg actgcccggt
    16081 gtatctcatt cacatgctca tttccggcca atgtccggta cacgtcgcca agccgggggc
    16141 cccacactgc cagggagtac tgctcctcca cggtccgacg tccggcctgc cccattcgag
    16201 cgcgcaactg gggctgccgg cagagtgtct cgagacgctc ataccactcc tgctcggtgg
    16261 cggcgagaaa tccgttctct ccgttcgcaa tgatgtcgcg attcactccg acgggggaag
    16321 ccaccgaagg cacccctacc gcaagatact gtaggagctt caaaccgcac ttgccccgag
    16381 cccactccgt gtcctcaagc ggcatcaccc cgatcgtggc atcctgcaga tccgccactt
    16441 cgcgttgaaa gtcccagggc ctgaattcaa tctcaacgcc cggcatgtgc ggcggccgcg
    16501 agcagaccac gcggagggtc aggcgaaacc tggattgcaa cgcgcgaagg gccggcgcca
    16561 gtacatccag atatttcagg ttatacgcca ggcctatcca tacgattgtg atggcatccg
    16621 agttctgtgc agatgaaccg gtggatctcg tcgaatccgg cttgaatcgt tccgtatcga
    16681 cgactgtggg aacgacacag acccgcggcg aaaactgctt cgcataggcg gccagccggt
    16741 cgttcccgac gatggcgcct gtggccattg agaggagcgc agggatcttc ttctcatgtc
    16801 caggagtcag gtagatcgca tcatccattt cgatcgccac tcgatatcga caccagcgca
    16861 gtagccgttc agcgagagga ggcgcataag gaaagagctg cccctcgatc acgatcaggt
    16921 cccgcttgcc cagggtcaac aacgtccaga gtcgtcggag gaaacagata agcacatagg
    16981 gaatcttcag caaagtccgt aacgcccgcg gatgaacctt cagaattgag aagtaggtct
    17041 ctccaaacag agcctcgacg cgacagccta ttcctgccgc ttgaaagtga ggcaggaatt
    17101 gaaagacgcg atagcgactg ctggggccga tgctcgaact cttcgagaaa tacgtgaccg
    17161 tcattgcacg tgtttctgcc ttgttgtgtt gaaatatccg ccggttgcgt gcgcacgacc
    17221 gactgctcaa atagtaccgg agttctcagg caaggatagg tttttcgttg tttttgacgg
    17281 gggagagagg cgccagactg ggaggacaca cgggtgccgg agggttcgct tcgcgacgct
    17341 tccggccctc agagcttcaa actccagtct tcccgcaact gggtaagatt tggattgtag
    17401 tatgggtccc ctttcattat aaactccccc cacttcagcc tgaatcttgc ctcgtcatcc
    17461 ggcgcacgcc tcccacgcct tgtcgcgttc tcatggtgat acagctctgc ttccggcgta
    17521 tagacgattc gatacccttg ctgctgaata cgaagacaga gatcaacatc gttgaattcg
    17581 acggccaatc gctcatcaaa cccgttcacc tgtcggaaca cgtctctcga aacgagcatg
    17641 caagcggcag tgacggcgct acagtttcgc agcatatgag gcagtcctct atagtgttga
    17701 gtatctcccc gcaggtgacg gaacgcatgt cccgccacgc ctctgatacc taacacaacc
    17761 cccgcatgtt gaatcattcc acttggatac aatagcttag ctcctacagc accgaccccc
    17821 gtacgttgcg cctgcgccac catggcactc atccactcgg gcgcaatgac ctgggtgtcg
    17881 tcattcaaga acaagagaaa ctctccgttc gctttcgagg ccccgtaatt gttgatctcg
    17941 gaaaaattaa acggtcccgg aaaccgatgg atgatccatt tttttgagag tgtgctcaga
    18001 tattctttcg ccttagccgt gcggctccca ttatcgacca caacgatttc gtaggaagga
    18061 tatcgtgtta cggattcaac gctcttaata cactgccgta gcatatccca gcgatctttt
    18121 gtgggaataa taatagacac cagagggttc cttgaaacac ggtagtgcac cttgatcttc
    18181 ccggcaccgt cacgcaccac atcagccgat tccccgcgac gattcaacgc gtcctgaaga
    18241 gatttcatct gagctgtcac atccaattca gactgaccgg cagtgatatc ggaggactca
    18301 tcagtgatgt ggcaatgaga caacacttga ggaacgcgtc cgatcttatt cgtctcttcc
    18361 gccacccgca atacaagatg atagaagcca tcgttcgcaa tgtctcccca atctgctccg
    18421 atatcgagga gaagttgctt tctcatgaca ttgaaacgac cgacataatt cattccaatg
    18481 aggagatctg gactccaaca aggcttaaag aacggatcta gaaattcacc gttctctcgg
    18541 accatatctt catcagcgta gaggaagtcc agatccggac attcttggaa gcgcgtgatc
    18601 acagaggaca tggcctctgg ggcaagatag cattccagag agagaaaact tacgaactct
    18661 cctaagcgcc ctttgagtat ccgatcagat atgcgctcct tctccgagat ctcttcaggt
    18721 tgaattctgg agtccgcttc ggaataggcg tttatgatat gttccatcat gcgcagtcga
    18781 gaaacctcac cgaccaccga caacgtccaa ttgggatacg tctgggcaat cagtgattcg
    18841 atagtctctc gcaatctctt ctcatcaatt cgactcacta ccagcacgat cgtaatatgc
    18901 ggtggtgtcg gcatttgccc tgccgcaaga cgcatccgct gtaagcattc tcgagatagt
    18961 gtgtggcgct tccgccatac ctcatactgg gctgactggc tttggagtgt aaaaggaagc
    19021 cccgaagaat ctccgaacgc aatgctgcgt actttgaata aacctttccg taggcatgct
    19081 ccaatccctt cattcgcgag cacttcccag aaacgggatc gtagccaata cagatcagac
    19141 aatatattca tctgaaacaa atgatcattc gggcgtaatc atctctctcg actatgagtt
    19201 ttcgcgcacg atctatgggc cagagtcggg accaggaaaa ctctgccgtc ggaaggaccg
    19261 gatcgtctta tcggcagcat catggccgac aagttactgg taaatacact ctctaggcac
    19321 aaagaatgcg tatcggcgcc taccactcat caccatctaa ccgttcgcga tgagtcgtct
    19381 gataggggaa atgagcattt tgataaagtc cttgagtcga ggatattgat tgatccaata
    19441 ccgccatgtc gtgatcgtac gccagccgtg atagcgagcg atcgagagta aatcgccgta
    19501 aatggatcgg cgaacgacgc cgtccccggc aaacgccctc aatgactgaa atccttgtat
    19561 agttgaaaga ttattcttag cccgcaatgt cgcctcaaga aattccagct tgaaacgcct
    19621 gtctggttcc aagtgacacc cttcggccca ttcgttccat gcattaataa agaccaagtt
    19681 gtcctcgttg ggataggtat tcaccgtctg atccaatgtc ttggccagcc aatgctcata
    19741 attatgcggc gttccattga gcacgactaa cgcgcgttcc cgcgaacgag ccgtattgtc
    19801 ccaggatggg aatacggtct taaagacgtt cgattcacgg tattgacgag tcaaatacgt
    19861 ttgagcaatg tcctggtact ccatagcatt gccataaaac tgatcgtgga attcgatgcg
    19921 gtcgttgacg ttccgacagt taagattgtg aggaggaaac tcgacgccac tatcaaaccc
    19981 aaactggcca tactgttcat tgccatgagt cagcgctgcg caaatatgaa tctttccgat
    20041 tcccactgtc tcacaataat ctcgccacac ctggaccgtg ctgcgggggt tcggcaaatg
    20101 ttgcggtcgg tatacaatta agaacggctc tcccctaagc ttgatatacc gtgggtcctt
    20161 aaagaatggg accagacttt taataaaatt caagtcgtca tccggtagat acttctgact
    20221 gataagaact tgatgctcag ccgcgtccca tcgccttgtc caattctcat ttgcccaaca
    20281 cagacaaaac ggcatatcac tttctcgatc aaccaacata tcgtccagcg gtcgattcaa
    20341 taaacgtgtg ccggagaacc agtaatagtg atagcaaaac ccatcaatgc cgtattgctt
    20401 ggcaaggcga atctgttcat gccgactctc tcgcaaacgg agatcataaa atcccaaatc
    20461 ggtcggtaga tgaggttgat agtgaccgtc gaacaatggg gacgctttcg ttacattggt
    20521 ccattccgtg aatccttttc cccaccactc gtcattttca ggtatgggat gaaattgtgt
    20581 taaaaaaaag gcaatcaacc tgggcccagg atgattgtct ccgttgaagc aaatgccggc
    20641 cattccttac tcccaatcgc tgtcaggcaa gctgcagagg cacggcgttt gttgcgatca
    20701 tagtcggata ccaagaagac tcagaccctg ccggatccgc tgtcgagtgc tctggcttag
    20761 atgatgagcg aggcgtgtct tccacaatgt ccgcatgcca actgcagtat gacgtggctc
    20821 cggaggcacc aacgagacgg catatccagg atggctgatg ggaacagcct tgacgaccac
    20881 ttgatagata tcggcgtaat cactactctt caaggcatcc tgcacggcgg cagaaagtcg
    20941 gcaccaactc gcggcgaatt cggtttcttc cggagtcttg acgacatatt cggcctcaag
    21001 gatcttgagg ccggcctgcg tgaacaagtc ttggatattt ttgagaccga agaagcggat
    21061 atgcgtacga tcgagcaacc cccattcacg atattcaaaa tcaccgttga tcaagcacga
    21121 cgcaactgcg gcatggccga catggggcag tgagatgacg agataaccat caggtcggat
    21181 aaagcctgcc atctgtgcca gaacgctcca gggatcgtac agatgttcga gtacatccgc
    21241 tgccaccaca acatcaaatg gtttcacttt atcgagcaag ctcggccact caggggaatt
    21301 caaatcccct cgcactatgg cttcgcaata tggcgcgacg cggtcgattg cttccgaatc
    21361 cagatcaagg cctgtcactc gacattgtcc atgctgcgtc agcagcttgg tgacggaacc
    21421 tggcccacag ccgacctcca ggacacgctt aaaatggcca accatccgta ccacataagt
    21481 cggcgcggat ggtgcagtga gatcaactga gaattcgtac ttgtgacgag gcttggtact
    21541 catggctctc caaatcgaga gtaggtgtga tgacagcaat cctctttgag gcgatcagtc
    21601 gccggccaag atttatccca ggcacttcta tcagcctata agtaatttca acgcaaggca
    21661 aaatagaaac tataagaatg ctcgaacata tgaagtagga gtaggctcca atggcgggaa
    21721 agcacaaagt atatatcttc agcaaatagc tctttagcgc aaaaataatt accggatgca
    21781 tcagatagat gctatagctg cgctcgccaa agtattgaag gaccccgctg gcaatgacac
    21841 gacttggtcg attcgcttgc caagcgcata atgcgctcag tccgaatccc cacagcacaa
    21901 tatcccatct cgccgaactg taaattagca atcccacatc agaaaaaagt aatcctgcga
    21961 taatcgcagt cgttgctatc ggtgcgtgca cacgcagcaa tgtcgaatgc cttccgtgaa
    22021 atttgaccac cagatacacg tagaggccca tgaggaaaaa gcaaatgttg gaagggaagg
    22081 agaagtagct ccagtcaaat ttcgatgatg attccacatt tgagccttga aggtgcagga
    22141 ctgaacgcag tgcgccgctg acaatgatgc tgacgactaa tagtaggaga agtagaagag
    22201 atggcttatg gggtttgacg agcagcagta agaccggaaa tattgcgtaa aatatcactt
    22261 ctacccccac agaccacccc ccccccaaac aatgccggag ggtggcacaa aaccaaatgt
    22321 gaaggttaga ttcagtagaa tttctgcagg gtcttttgat ccggctcctt tgacgacccc
    22381 aacgacaacc cagaagacaa tcatgaggta aaatagggga gcaatcctga aaaatctttt
    22441 tacaaaataa tcacttagcc aacttggcgc ggtcgtccgc gattcggttg aatgcatcaa
    22501 cgagaatgcg ctgagtatga aaaaaagatg tactccatag ccgaaatcgc gacctatgaa
    22561 ttggaatgaa gttggagggt ttaagcctcc cacgtggact aggtgaaaaa acacaacggc
    22621 aatagcggca attcctctga gaccgtggat gccgggtagc agccgtccgt cctgcacaag
    22681 ggttgaattg aattggccca tggttacagc tttataggat caagcggttc ctatagccag
    22741 gctccgaact gcttgcctcg caacaaatag tgttcaccaa tatcaaagcc atccggagac
    22801 agcttgctga ccccccgttt gacgatgacg taattgtgat tgacgaaaat atcttcgatc
    22861 aaatcgcacc tactggcggc gagcatggtt agctcgatta gcagattgct caaggcacgc
    22921 ttgtccttga aatgaggatt gtctttttgc caatagtctc caggccaatg agcccaggcc
    22981 cagtcctcaa tgacatagtg cccacctggc ttcaggtatg gaaagctaat gttaaatgct
    23041 tcacgggttt cttcgtacag atgggacgca tcatcaatta tcaagtcgat ctcttggttg
    23101 ggaaactcgt acgccaaaat ctttcgcatc gcacgccgat ctgcttggtt cacgccataa
    23161 tacggtttga tatggtccgc ttttttatgt cgatgaatat attgcgtgag tgcatccacc
    23221 ggtcttgaca tgaattcgac ggctacgatc ttttccgtcg gaaaaagttg atcatacagc
    23281 gctacacttc cgcccttgaa tatgccaata tcaaaaacgt tgcaaatctt ttgtcccgca
    23341 agaacagtca tcaatttttc gatcatggac cggctcttgc ctaggagaaa tttgtctacc
    23401 gtggaattta ttccatgtag ttcttcaggg ctgtaagcaa cataaaaatc cacaccattt
    23461 actcttaaat tcgagtcatt ctcccaaact aaaattggcg agctcatttt tcccttccct
    23521 taagattagt gtggcctaac gggtggaact ttctaagtaa agcacccatt acccgcagtg
    23581 gctcggtcac acgccaacta gcagatgata aaagtgcagc gatgtactcg tcccgctgaa
    23641 ctagcgtctg cttgagattc acaatctgcc tgtcctgctc cgccaaggat tgggtcattg
    23701 aagcaattag ctcatcccgc tccgacaaac accgattgag ctgagcaatt gctcttttgc
    23761 tcaattcgag caccgcctga tcgagtcgag caatctgcac atcaaaggac gccacgactt
    23821 cgtttgtcga aacaatctct gactgcccct cgggcacaga agacttcctc caatagactc
    23881 cgacaccatc aatttccaca atttgcggcc ttatcgcgtt tttttcacaa tagtcactta
    23941 cagccgcttt acaggctggt accacatggt agtcgtcgac aatcacgtac cctaggcgtg
    24001 agagctttgg atacaggtaa gtcaatgcgt ccatagttga ctcatacatg tcgccgtcta
    24061 atcgcaacag tgccaatcgg tctatcggcg cttttggcaa actatccttg aaccagccct
    24121 tcacaaattt aacctgcgca tctaatagcc cgtgagcacg gaagttttct ttgacttcct
    24181 ccaaagacac cgccagttgg ctataagtat gaaaatcgga cccagcatcc gctgggtatt
    24241 gaagcgtgtt gccggccggc aagccttcaa acgaatcagc aacccacacg gacttatcac
    24301 tcacgctgtt agcatacaga atggctcgca taaagataca cgcaccgccg cgccacaccc
    24361 ctgtttcgat aaggtcgccg ggcacattgt cggcaatcac ggattccgtc agtgcccgca
    24421 ggtttgcaag gcgttttaca ccgatcatcg tctctgcagt tgaaggccaa tccaggccat
    24481 gctctcgacg atgcgaatca aacagatttg atccaaaagg tgcctgagca aaatctctgt
    24541 aaatactgcc tgtcaaaaac gcttgcatga ggtcaagata ctcgtttcgc acacgcatca
    24601 atttatcttg agagcctacc aattccgcgt tcatatgttt actcactggc taataaccac
    24661 tcagatagtt catagacaag tttcgcacgc cagtttcagg ctaccgccgt tttcgagaat
    24721 ggcgtcccct gactgctgag ctcaatcgaa tcaaaaacca aaccgaccaa accactgacc
    24781 acgcttgacg tgtgagattc cagactgaat gcatcatgta gccattgcag ttgggtgaca
    24841 ctttcgggag aagcgccgtc cgcaattgcc acatccaccg aatacttgcc gcgtggcaat
    24901 atcggcaaac gaaaaacaaa aactccctta acagtatcat tcggacttgc tgaaactgga
    24961 ttcatgcaat atgcggtaaa ggaattttgg gcaaacaata cttgaccaag cctatcctta
    25021 aactgaaacc cgataattgt gttcttacaa ggcactgtga ctagagcttc tatttccaac
    25081 cgtaccatct caccaccaac gacccaagag agttgcctgc cttctaaatc tgtcacatgg
    25141 acatctgtga tcgaggcgcc tccatttcca aaaccatgtc tttccgcgga aaggtccacc
    25201 acctcaatgt catttcgtaa atttgagtga ttgatgaaat caatgcgcgc atcatgcttt
    25261 gacaacgagc gttgttctgc atttgacata gacacttccg ggacctgcct gggaatggta
    25321 ctgcgcacag caccataacg attcgcaaga tattcttcgc atacctgctt tgcttgacca
    25381 atcgaccgta cctcaccctg ctccaaccat attgcacgat cacagaggcc gacaaccgtt
    25441 ccgaagtcat gtgtcacaag tacaattgtg ccgtagcttt taaaagtgcg caaaaatcgc
    25501 atgcatttct gcgcaaaaaa aacatctccc accgccaatg cctcatcaat aaccaagatt
    25561 tccgcatcaa catgcgcaat gacggcaaag gccaggcgga caaacatgcc actggagtat
    25621 gtcttaacag gttgatcgat aaagtcaccg atttcagcaa aagcaacaat ctcgtcatac
    25681 ttagcatcaa tttccgtttg agatagtcct agtatcgaag cgttcatata gacattctct
    25741 cgcccggtaa attcggggtg aaatcctgca ccaagctcaa gaagggctgc gatgcgtcca
    25801 tgaacttcaa cacctccatt cgttggcata agtgtcccac agattagttg gagtagagtt
    25861 gattttcctg atccgttgcc tccgacgatt ccgacagttt cgcccttcct aatctctagt
    25921 gagcagttct tgagcgccca aaactctcga aagtagggca ttgaagaaat ccccaacact
    25981 cttcgcaacc tcgggataaa ggactgcttc aaccgatcaa caggcctgtg atagaggtga
    26041 taacatttac tgagatcttc tactctgatc gcgatgttag aggacatccg cgaacccctt
    26101 tcgtgtacgc tgaaaccaag ctagtcccaa ccatgccact acagcgctac acagaaagta
    26161 gatcactacc caatgccacg aaggcattct tcccataatc aatacattcc gcgattgctc
    26221 cacgacgaat gtgagtggat tcagatacat aaacggacga taggcatctg gaagcgaaga
    26281 aagcggataa aacacagggg caagaaatag catgagattg gtgacgatgg tagtggcatg
    26341 tcccacatcg cgcacgaaca ccccgactga ggctagaaac cacgaaacgc cgagcgcata
    26401 caatgccaac ggaatgacta agagaggcaa catagtggcc gtccaatgca gagggaggtg
    26461 gcttgaaata tggaacacga gaagcacggt tatgcttacg cccatgttaa gaaatgcttc
    26521 tcctaacgac acccacggga gaatctccag agggaatacg atcttcttta catagctgac
    26581 gtgattcaga ataagggtgg gagcacgatt gacacattca ttaaataccg cgcacacgat
    26641 caatcccgta aagagggtca tcacaaattc tgaattaccc tcgcccccat ggctccatga
    26701 cgccttaaac acaactcgaa acacaaatgc gtagaccacc agcatcatta ttgggtgcgc
    26761 tagtgaccag agcaccccta ataatgaccc tcgataccga ctcgcaattt cgcgcccagt
    26821 caacagtcga atcagattcc aatgactgta cagactggtg aataccatga ggggggagag
    26881 actataacgc tgagatccca ttggcggcct ttgctacgta aactgcggct cgatcatcaa
    26941 tcgagcgggg atgatcgtat caagtcttct tgcggcgagc ggcacgtccc ttccttggga
    27001 gctagacaca gcacgacgtt ggatcagaaa tcccgctgct gaaacactac gcaagcactg
    27061 atgagtaaaa ccccggcgta catgaccccg tagagagacg ccaacatgac atactccggc
    27121 gcggcaacaa cccccactgc agcctgcccc ttgatgttca gcatttcgag gtttgggcag
    27181 agatagtaga gcaggtctac gagagatttg atcgggccgc tgtcgctatt ggcgaccatt
    27241 gcccgtaggt cggccgtcag atggccgatc acatacaggc ccagcgtgaa gatggcgctc
    27301 aacgtcgtcg atgtgaacgt cgaaaaaaag agggcaatgg ccgtcaccac caaaatttcc
    27361 acgaagatca gtgccaccgc ttgaaaaatg gatggctgaa tcgacacatg gtagagccac
    27421 agggtcagaa gaaacaccgc ccacatgatg gccatgttca cgaacaacgt cagcgccagg
    27481 ccaaggtact tgcccatgat aaaagaggca cgactgatcg gcctcgccat aatcgtgtag
    27541 atcgtacgcc gctcgatttc tttattgacc aggctgatgc cgacgaaaat agcgatgatg
    27601 acgccgatca ggttgatggc cgctaaaccc atatcgacaa tgaccttgtg gtgctcggta
    27661 atcgacagat ccgccagcaa cacggacagc ccgataagga ggcccgcgaa cagcaccaga
    27721 ttgtagagaa tcttgtctcg caaactttca cgaaacgcat ttaaggcgat gacgccgatc
    27781 gcacccatta agaggccctc ccactggtca catgcggcaa tgacggggag gtccgttggg
    27841 tggcctcctg aaaaaacaaa tcctccagcg atgctttatg aggcgttacc gacaacaatc
    27901 gcccaccctg ccgccgaatt tccccgacga gcgcatcgac tgcgtcaggg ctgggcaaga
    27961 cgatcagaca ctgttgcccc tgctggagca cacgggtggc caacgagtga atgaaggcat
    28021 tctcctcgac cttgagctgc tggcagacca cttcaacgga ctgcgtatga tcctgccgga
    28081 ccagctcatc gactcggccg ctggcaacga gacgaccttt catgacaatt ccaacccggt
    28141 cacaaatcat ctccacgtca tgcagaatgt gtgtactgaa aaagacggtc ttcccgcgat
    28201 cgcgcaggtt caagatcaga tcgcggacct gcttgcgtcc gaccgggtcc aggccagtca
    28261 tcggctcgtc gaggatgatc aattcagggt cgtgaatcag cgcctgcgct aagccgacac
    28321 gctgcaacat ccctttggag aatttgcgca actgccgggt acgggcgtct gtgaggccga
    28381 cgagccccaa gaggtccgtg acccgctggg tgatggcact gcgggccaaa cctgccaatt
    28441 gaccataaaa ccccaaaaac tcttcggcgg tgagatagtc gtaaaaatag ggagactccg
    28501 gcaaaaagcc aattcggcgc ctcgccgcca catcaccggc cggctgcccc agcaagtacg
    28561 cctgaccgct cgttgcccga acaagcccca gcaaaatctt cagcgtggtg gtttttcccg
    28621 ctccatttgg tcccaggaag ccgaaaatct ctcctcggcg aacggtgagt gacaatccgt
    28681 ccagcgcgac gaacggcggt cgcccgggcc aaccggaggc gtaggatttg gtcaagttgt
    28741 cggtgacgat atcatccaca gcgcgcctcc attctcactg cacagtcggt aacggagtct
    28801 ccatcgaacc ggcccctccc tccgactgcg tcgcacgtcc caatcggcac gcgactttct
    28861 catgaatccg aagtcgctcg cgcgtggagg aggcgctgac ggttccggtc aaactattga
    28921 gctcatactg cccgcccaac ggatccatcg gcaggctggt gatgataccg cgcaacagaa
    28981 gatcttccag ttttgccggc gcctgcccat agtgcgctcg gtaccgactg atcccctctt
    29041 cgagaacgcg tagatcctgt tcttgcacga tttctttcat ccgccgaaac aatgcctctc
    29101 gcacccgctc atccgtgacg ctccgggaaa aacgctccag gaattccagc gccgcggagg
    29161 agtccccgct ctccaccgtc atacgtgcgg ccaattgcgg aaggtacgca ggcgcaccgg
    29221 gcactcgcgc cgcgatccga aaaaattccc cacccgccgc cggatcacag cgctcgtagt
    29281 acgagatata gccggccagg aacggcagct gccaatgtga cggattgtgc tgaataccct
    29341 tcttcaggat cgccacacct tcttcatgcc gaccgaccaa gacacccaga aagagtccgg
    29401 tggcttggta gggcggaaca aacgtcggat ccaaatccgt caggacatcg acggcgtgat
    29461 atgtccacat ataccccgcc tgcgtatccc gcttcgcgcc gatatgttga accgcctgca
    29521 gccagatcag gtctgcgacg acttgccgat acccgagcac ggccaatttc aaatagtctc
    29581 ccttcgggag ataggccaat tgttcagcca gtgcgacgga agtccgctcc tgatcgagaa
    29641 catggagcac gcccgacgcg ccggccagca atgccagtcc gcataccacc tgggtcatgc
    29701 gggacttcgt ggccctgctg aatctcattg gcctcacccg gcgagcccct tctctccaac
    29761 cgcggcacgg agcggagcca catcaacccc taagaatgcc cgatcgatac tgttcttatt
    29821 ccactgctcg taccgagcct gccgtgcctg aatttcatga agttcctgtc tcgcctcatc
    29881 gattcgaccg gcatcgaccc acacatgtgc cagcaatgca cgagcaggca agaaattcgg
    29941 ctccaggcgt tcggcctctt tcgcctgctg ctcggccgcc gctttctccc ccagcatcca
    30001 atggagtcga gcctgttcat accgataagg agcagaaaac ggcgcgagcc ggacagcccg
    30061 ttcgtacaca cgcaatgcgg cacgtatcca ggatttccgc tgttcgtcga gaccgcctga
    30121 caaggatggc gcctgggccg ccgacacata cagctggccc aagagtccca acaaccggct
    30181 gtccaacgga ttcaactcaa tcgcctcttt gaactccgca tgcgccaacc ggaacatctc
    30241 tttgtcgcgg gatgcgtcga acgctctagc atagaccgac cccagaccat ggtggtagag
    30301 cgacttacca gggtccaacg caatcgcgtg ctgtagtcct tgaatcgcgg catctgtttc
    30361 gccggccacg gcccgtcgcg atgcagcatc gaaggtcagc caagccatcc ccaaccgact
    30421 gacttccgct ccgaccagca gtaacacaca ggccgcactc attccccaca caaaacgcga
    30481 tcgaatcggt atgacctgca cggcatcggt gctgcaacgg gtcatccgag cagctgaaac
    30541 aatcagtccc gcgcagagaa cgagcagaat cgccaacgcc gtttcgcgca agctggaatc
    30601 gagcgcggca tggaccagaa gcgcgatccc tccggctccc aatccaagga tgaggcttct
    30661 ctgccggcga gataatcgcg tctgaaatac gcgtgcgaag tctcgcccca ccatgaatac
    30721 gccggccaaa aacagcagca gcgcaccgac gcccatttcg atccccattt gcaaataatc
    30781 gttgtgcggc gtttgcgcga cttttccata ccgcgcaatc tcaccttcaa ccggaaacgc
    30841 atagcgaggg taggtgtatt gatacagacc gagcccgatg cccagtggat gctcagccat
    30901 ttgcgtcaac gccccttgcc acatttgcaa gcgcgcatag gagacgggat tctgttgatg
    30961 ttcggcctgc acccgttccc gtaacggcgt gggaatgagt agaccaccca ccatcaacaa
    31021 caccaaacaa ctcccggcca ccttccagcc gtatcgcgcc atcacaataa agaccgtcgc
    31081 cacaaggacc actatcatgc caccgcgcga ttgggtcagc agcaccgcaa ggaataggct
    31141 gcctagcgac gcacccacac caaaccacca taacgtcggt gaaatgggga gccagtgcag
    31201 tgccgctgtg cgtcgatatc catacactgc ggcacttagt aggatcgccc aactgacggc
    31261 gagatacccg gcaagaaaat tggggttaaa gaacgtaccg ctgggtctca tgtcattcca
    31321 gacaaggcct tggagaatgg ttagggcggc ttcaccgact cccatcagaa cgaccactat
    31381 cgtcaatgta tgaacatgct cccaccggtc cacaaaagag acaagcaaat agaacaacgt
    31441 cgcataacag atgatcgtga acagccattg tcgactggga tgagcatagg gagaaaacat
    31501 ggtggcgatc accgcgaatc cgagaaatac cagcacgaaa tatctgaccg acagcagggg
    31561 tatcgtcaga cgcccctgcc ggatactcag ggccagcgcg cctccagccc acgcgagaat
    31621 caggaaacgg atgatcatct gcgcgacatg cgtggtgccc ccctcctgga gcggagaaaa
    31681 gatcacgagg gcgctggtca cgaccagcgg aatggatgac aggaattgca taggtgtgct
    31741 tcacaggctt gacgcaatcg atacctcctg ctttatcaga gaccgacgat tcagcaaagt
    31801 gaaagtgaaa ttagttactg cggcgggaga caaacgtggc aggaaactaa acgggccata
    31861 cagcgaacag tccccgtccg gtcatcagaa atgaacacag gggaggaacg ttgttcctcc
    31921 cccgtctctg agcacactca cgagtgatcg ttagctgacc acgtcgtcta tatcagctgc
    31981 agtgagtccc gccttgatgt cattgacgtg ccagctgtcg atcgtcgcgt cattgtcgat
    32041 gctggccacc gccgtcgcgg tgaagccaac cgagcccgcc accgtgcttg catttgacgg
    32101 aaaggtcgca ttatcacccg ccgccacaca gggcgcagga gcaccacaga caatctggtc
    32161 atagttggca gcaggatacg ccacaggtgc agacgcccct gtaccaccag ccacagggct
    32221 gcggtacgta taccggttgg tcgtacccgc aagagcgtag ccgatttccg agaagctgcc
    32281 atagcgcgag ttttcgctga gataggccgt ctctgccacg aaaatcgcgc ccaggttggt
    32341 cttggcttcc gactgtctgg acttggcctg gtagcgcaag aagttcggaa tggcgatcgc
    32401 cgccaagata ccgatgatcg ccacaacgat catcaactcg atcaacgtaa aacctttttg
    32461 acccttgatt tgcttcaaca tacttcctcc ctttcctatt gttgccactt catccgacac
    32521 gttcttctct cttgcctctt cgtacgacac agcttttttc aagcctagca gatcttggga
    32581 atagcagatt ctatgcccaa cacctccttc ctgtagttcc ttgtcatttc ttctattttc
    32641 cagttattta gatccattat gccctcaact tctggtcccg ccgcttgaac cccattccat
    32701 tttccgtcat cttttgacaa gttttgcgct ctcactaact cgacacatca ttgggctccg
    32761 ccccattcaa tccctgcttc gcatcgttca cataccagcc gtcatgcgtc gcatctccat
    32821 cgagatccgc cgcggcggtg gccgtaaatc cagaggcgga tgtcgtcgcg ctaccaaccg
    32881 ctcccgtata agtcattgcc cccgccatcg gagacgccgt ctgaatcgtg tcacagctac
    32941 cggatgaccc gcagagattg cccccgttcg gtcccatccc ggatccaatt cccaggccgg
    33001 atcgataggt gtagcggttc gtcccgcctt cggcaatggc aaagcccaca tctgtaaaat
    33061 tcccatattc tttccgctca ctgaagaacg aggtttcggc cacgaagata cccgccaaat
    33121 tcgaccgggc ctcagcctgc atcgcctgcg cccggtatcg aagaaagttg ggtatggcaa
    33181 aggcagcgag aattcccaca atcaccacca cgaccatcaa ttcgatcagc gtgaaccctt
    33241 gttgcttcga tgtagtcacc atgaggtgta atcacagatc ttgaggacac ccattcatat
    33301 gctcccttgc cacacgcgcc ctgcagtcag gccttccata agcaggcctt atgccacgaa
    33361 accgccgagt gccgggaaga ttgataagat cacgtcaaca ttgatctttt ctctccgatt
    33421 tcacagcgat tgccgtccga cagtcccccg cggacctgaa ttcataggag aaagtgccaa
    33481 aaattgacgg aatcgaacag tcacggctcc cactcttagg gaacggggac agccggtccc
    33541 agcgattctg ggctggtaaa gtgaaacccg acggagacga tctgttcggg tgtggaaagg
    33601 ccgcaggcga tgcagcccgg ttcgcagatg cccgatggag cgaaaaagat ggagggatta
    33661 ctgctgagcg ggagccggag tggcaccctt gcgatctgag acgcgccgaa gcgcctggcg
    33721 aatgcgttct tctttggcgg gatcgcccaa gcccagttca cggaatcgct gcatcagctt
    33781 gtcattggaa ggatccagtt ccagcgaatg caggagcgct tcccgaccat ccgagagatg
    33841 ttgctgcttt aagtagattt cgccgagatg ttcataaatc acgggatcgt cgccgaccag
    33901 tgcgaccgcc cgcttcatct ccgccagcgc ttcagccagc aggcccttct tgaagaacgc
    33961 ccaggccaga ctatcgacgt aatacccgtt ggtcggtcga agtgcgacgg cctgcttggt
    34021 gagcgcaatc gcctcttcga tcttcacacc gcgttctgca tagctatacc ctaggtagtt
    34081 catagcatcg gcgtggtgcg gatccaaggc gagcgtcgtc tccatcgatt tcacgacctc
    34141 gtcgaatcga ttcagcttgt catacgcggt tcccgcatta aaatgcaggt ccgcattgtc
    34201 cggattgtgc cgaatccctt cctgaaacgc ctggagggaa ggctcgtact gttcgacttg
    34261 aaagtgcgac aaccccagga cgatatgggc ctccggctgc ttgggattca accggctggc
    34321 ttcgcgaagg tgctgaatgg attcggcata ctgcttggtc cggtactgca ggacacccaa
    34381 gtgtagatgc ccctcgaaat acgaaggctc cagcgtcaga ttgtaacgat aggcctccat
    34441 cgcattggcg taatccttgg tctcttcgta caaatacccc aggtagtcac ggactttcag
    34501 ttccgtcggg cgcaccgtga ggatttgttt caactgctga atggctttgg ggtagtcttt
    34561 ctgctcccca taaacaagcc ccatccgaag ctgagcatcg aggtctgacg gatcttcaga
    34621 taacatttcc tgcaattcac gcagggcttc gtcgtactgc ttcgccgaca cctgcagacg
    34681 aataagatga tgccggatgt cacgattttt cggattcaca ccctgcaggt agcgacgata
    34741 gatcccgatc gccttgtccc gatcctgctt ggcttcatag acagacccta gggccacata
    34801 agcgggctcg aaggctggat tcagtgccac cgcctgctcg aagtgagtcg tcgccttgtc
    34861 aaaatctctg gcttcgaccc cgatccgacc taaatagtag tacccgactg ccgagtccgg
    34921 gctgatcttg attccagcgc gaatcgtatc ttcggcttca acatattgtt tctgattggc
    34981 ttgcaacagc cccttggcaa agaaatgctc gctacgctga ggttcctgct caatggcacg
    35041 attgaacaat ctcaacgcct tgtcgggctt tcctgctgcc gcatacatgc cgccgatttg
    35101 ccccagcatc tgcgcatcca gccccggcac gtcggccact tcatccgcaa agcgcaccgc
    35161 cgacggcaca tcccccgcgg tgaagtagag cacggccagc ctggctttga gatagtttga
    35221 agcgggatcc gtttgtaggg caagttgata ttccttgatc gccagttccg tctcctgatc
    35281 caactcggcc tggtatccga ggagaaagtg gtaggacgcg cgagaatctg aggatgaggg
    35341 catgggacga ggagccaccg gcacggcggc tgggagcgca gcaggagcgg gacgggcagg
    35401 cggtgtgctg tggcaggcgg cggtgacaca ggccatagcc gcgagtagaa gagctaccgt
    35461 tctgacattc gcagaacccc ttcgggcggg tggctgcggg cactcttcac acaatcgttg
    35521 cacaatcatc cgctcaactt gcagggggaa tattcgaagg acagtgactg acgggaatta
    35581 taccggcccg agaaagaacg cgtcaaacga cctcgcgact ctcgagattc tcgaacttgg
    35641 caaaccggtc gtgaaagaat aagtcccgtt tcccgatcgg tccgttacga tgcttactga
    35701 ccagaatttc ggcgatgcct tttctctccg tcgccggttc gtagacttct tcacgataga
    35761 taaacatcac gacatcggcg tcctgctcga tcgcaccgct ttcccgcaag tcggccaaca
    35821 tgggaaccgg cggcttccgg gcttccactg cgcgactcaa ctgtgaaagc gcgatgaccg
    35881 gcacgttcag ctctttcgcc agggttttta aggaacggga aatgtcggaa atttcctgct
    35941 gccgtgactc ggagtcgctc ttcccttgca tgagctggag atagtcgaca atcaacagat
    36001 cgaggccacg ctcagcctta agccgcctcg ctttcccgcg catctgctgc accgtcacgg
    36061 cgccggagtc gtcgatatag atcggcgcct gctccagctt cccggccgct tccgcgaggc
    36121 gccaccagtc ttccttttgt aaccgaccgg tccggagcgc atgcgaatcg acgcgggcct
    36181 cggaactcaa catgcggagc acgagttgcg gcttcgacat ttcgaggctg aaaatgccga
    36241 cgacggtgcc ggcatgcagc gcggcatgct gcgccatccc aagcgccaga ctggtcttgc
    36301 ccatactcgg acggccggca atgacgatca agtccgacgg ctgtaatccg gcggtcaggt
    36361 catccagatc gataaagccg gtcggtacgc ccgtgacacg ttccttgcgc ttggacagct
    36421 tgtcgacgac atcgagactc tctttgatga tctgattgac cggagtaaag gaacgatcca
    36481 gctttccctg agcaaggctg aagaccgaac gctcggcaaa atccaacagc tcatcgactg
    36541 ccaccgtccc gtcatacccg cgcgtcacga cctctgtcga ggtctgaatg agccctcgca
    36601 acagcgcttt gtcccgaacg atcttgctgt gatagcggac attggccgcc gtcggcacca
    36661 cctgaacgag ctcggctaga taggcggatc ctccgaccgc ttcgagctca gagcgcgcct
    36721 tcaagcattc cgtgagcgta atctgatcga tgacttcgcc gcgatcggac agctccagca
    36781 tggcttgata aatcttgcga tgcgcagtcc ggtaaaactc ctcgtccgtc agcagctcca
    36841 tcaccttggc catcgccgtg ttatccaaca agatcgcgcc gagcaccgac tgttcggcct
    36901 cgacgttctg tggcggcaat ttgggggagg tgagatcaac cgcggagagc gacttcatac
    36961 ccggctcctg cgccgtcgcg gttctgccgc aaccggcttg gagccagcgg ttgtagaggg
    37021 actggagtga ggcggcgtca ccacaccgga agcatcgagg gctcgaaaca gacgatcgac
    37081 atcgagccca aacccggtag aagccgcggt ccgaccgaac cggcccatca aatggtcgta
    37141 tcggcccccg cctcccagct ccgccccgat acccggcgca aagacgtcga agactatccc
    37201 gtcataatat tcaaacccgc ggaactcgcc caaatcgagc aacaccgcct gctgcccggg
    37261 cgacaccaat cgctcataga cctgcgcaag gcgatcgagt ggtctcaaga gggtccggtc
    37321 gcgccccacc aactttctcc cgtgcgccaa gacctcaggc ccaccgcaca gttccagcgc
    37381 ttcaagaatg acccgccccg tcgatcggga cacgccctcc cgcgtcagca gttcctcaag
    37441 cagcggcatg tctttccgcg cggcagcctg ctccacacgc ttctggccgt cctgcgataa
    37501 gccggccttg accagcaggg ccgtaaaaaa tccgacgtgg ccgacagcaa ccttgaatga
    37561 agacaacccg acctgtgcca aacattccag taggagcgtc aacacctcgg catctcccgt
    37621 aaccccgtcc acgccgatga gttccgctcc gacttgaaaa atctcgcggt cgcggcccgc
    37681 atgctcacgc tcataccgga acacagaggt gcgataacac aaccgcagcg gaagccgctc
    37741 gcccatcata cccatggcga ccgttctggc aatctgtgcc gtggcatccg gacgcagcag
    37801 catcaaccgg cccgtcgtcc gatcgaccag ctgataacat ttctcgataa gttcggactc
    37861 caggcctggc gcgaggacgt ccagatactc gaacatcggc aggatgattt cttcgtaacc
    37921 cgcgcgagcg agcacggcca gcagcgtctg ctcgagctgt cggatacgtc gagcggcgtc
    37981 aggaagaatg gtgctcattc caacgggaat gagcgaccgt tcccgacctg ccaccagagg
    38041 cgaggaggaa gaggaagcct tcaacgaaag agcgcccatg gtcgactgcc gggcaatcta
    38101 caaccctttc gacaggtcgg cgaccttcac ggaaaggatg tgcttgctgt tcgtaatctg
    38161 atcgaggact gacttaggaa gcggcgcatc gagcccgaaa atttgcaggg ctttgccgcc
    38221 acgctcttcc cgtgaacact gcatgcgcgc aatattgatg ttgtgatcgc caaggatatg
    38281 ccccaccgtg ccgatcaccc ccggacgatc ctcgttctgg atcagcagga gatggttgtc
    38341 cggcaccact tccaccttga actgatcgat ctcaatgatc cgcgggtcct tgcgatggta
    38401 gagggtcccg gcgacttgat gagatttctt cccggcctca acccgcacac ggatcacact
    38461 ggcaaagtct ccggcatcgc tgatcttcac ctctttgact tcgatgccac gttccttggc
    38521 gacgaccggc gcattcacat agttgaccgg gtcttcaaga atgggggtca ggagtccctt
    38581 cagaaccgcg atcgtcaatg gcgccacgtt caaacccgcc acttctccgc tatactcgac
    38641 ggtcaaccgc tccagcccgc cttccaacaa ttgcgcctgc aagaggccga cacgctcccc
    38701 aagcgaaaga tacggctgga gtttcggtaa gagctccggt gagaccgagg gaatattgac
    38761 cgcaccccgg gcaatccctt tggtgaagta ttccacgatc tgctcggcaa tgccgatcgc
    38821 cacattttcc tgcgcttccg tagtggaggc gccgatgtgc ggcgagcaga taaaattatc
    38881 caacgccagg agcggattgt ccggcttgac cggctcgtct tcgaacacgt cgaatgccgc
    38941 ggcggcaacc ttcttggatt tgagcgcctc gcacaaatct ccctcgtgca cgattccacc
    39001 acgcgcacag ttggcaatca tgacgccggt tttcatcttg gcaatggctt gcgcattgat
    39061 cagggctttg gtttccgggg tcagcggagt atgcacggaa atgacatcgg ctcggcggaa
    39121 cagctcgtcc aactcgacga tggtcacacc cattttttcc gcacgctccg gggccagata
    39181 gggatcgtag gccatcacct gcatccccac tccctgtgca agcttggtga gatacccgcc
    39241 gatttggccg acgccgacaa tgccgagcac tttattgtac aactccacgc ccatgaactt
    39301 ttctttttcc cacttcccac cctttgtcga ggcggtagct tgcgggatgc ggcgcgacat
    39361 cgagaaaatc atggccatcg tatgctcggc agtcgtcacg gtgttgccgc cgggtgtatt
    39421 catgacgacg atgccgcggc gcgtggcggc cggggtatcg acattgtcca agccggaacc
    39481 ggctcgaccg acgaccttca gctggctggc ggccgcgatg acttccgcgg tgactttcgt
    39541 cccggagcgg acgatcagtc cgtccgcgtc cttgatttcc ttcagcagct cttctttcgg
    39601 cagtttggtt ttcaccacca ccgtaaagcc ggccttctcc aatacctcga caccctgctt
    39661 cgagagacta tcgctgacca aaattttcat tgtcgtgacg ccttacctga cggtgagtct
    39721 aagacttgcc cataataatt tcttgagcct ttgccacccc gctaccgagt tttatgggat
    39781 agcccagccc cttgatgacc atctccaccg ccgccagggc cgtgatgacg tcaaaactat
    39841 cgatataccc catgtgagaa atacggaaga ttttgccctt caggtgatcc tggccacccg
    39901 ccgccgtcat tccatattgg gtccgcaaat tcttatagac ggcctgtccg tccacacctt
    39961 caggcgccga aatcgcggtt aacgcgtcgc tcggacgctc ttgagggaag atggagagcc
    40021 ctgcggcctt gacgccttct cgcatcgccg tcgccaacat cgcgtgacgg gcaaagacgg
    40081 cttctagccc ctcggctttc aacatgttca ttacttcttt gaggcccaga atcagggaca
    40141 cagccggcgt ataggccgtc gtgctcttct gctgattctc acgctcgcgc ttaaaattga
    40201 aatagaacgc cgcgttcttc gctttgtcgg ccaatcgcca ggccttttca ctcacgctgg
    40261 caaaggctaa gcccggcggc aacattaagg ctttctgcga cccggtgatg actacgtcga
    40321 ggccccaggc atcggtcttg agatcgagga ctcccagggc ggtaatcgca tccacgacca
    40381 aaatcgtctc ctcgtaccct ttgacgatat cggccaaggc tttgacgtca tgcgccacag
    40441 ccgtggaggt ctcgctcgcc tgcacataga cggccttgat cgacggatcc ttcttcaagg
    40501 catcggcgac ggcctgcgga tccaccgcac ggccccactc gacctttaac tcagtgacct
    40561 gcgctccgaa cgtcttacac agcttcgtcc aacgctcgcc gaacttgccg ccgttgatcg
    40621 tgagggcctt atcccccggg gacaagaaat tcgaaaccga gccctccatc cctccggtgc
    40681 cggaagacgc gagaatcaac acatcgttcc tggtctgaaa cagccacttc aggtcctcac
    40741 ggacctcggc aaacagttta tcgaattcgg gcgcccggtg atgaatcatg ggtctggcca
    40801 tggccagtaa cacctccgga ggaacggggg tgggaccggg agctagcaaa taccgtttga
    40861 gcatgggacg aacctcctca ctgtgcagat cgaacgatgt cagggattta gaaggggcgt
    40921 acgctaccat agcccctctg aggtgtcaag gtaacgaacc gtgaagatgc ttatcttttc
    40981 agcgacttca ctgcatcgcg caagatttct ttcagagacg cagcgcgatc tcctgcgcac
    41041 tgttttctcc ctgtaaacgc gatgacgact caactccctc aatttcttga caagttgtac
    41101 cctcctcgat aggctaccct tcgagagtca gcattctcac atttcctgaa cagtctgatg
    41161 ggtaccctta tgcatgcacg tttcctgggc acaggtgttc gcgctctgcc ggttctctcg
    41221 ttctgcctgg tctctctcgc gtggccggcc ctcgcaggag cggagtcgcc caccccatcc
    41281 cacgccgaca cacccgcgac tacggagact acgccggaag acattcaacc atccattcag
    41341 gaaccggaat actcgttgga cgaagaagac cacgccactg ccgcggaact cgaagccgtt
    41401 gagcccccgg cccccattgc cagcgaaccg tcgaccgaag tcccgccggc cgaagcgctc
    41461 cttcaactca agcccctcaa tggcaccacc gctcgattca cggatctgct gacaccaccc
    41521 gcagaggtgg ttcaggaaga ggcttctgcg caggaccagg aatcggatga agccacggcc
    41581 tacaacgtgc cgattgtgct cgacccatcc gttcaaggcc atattcgttt tttcaacgtc
    41641 tctatccgca atcgatttga acagtggctc atccgcctga gtcactatcg cccgctggtc
    41701 gacagcatct tctctgaatt ccagctcccc agcgatctca tctatctgtc tctggttgaa
    41761 agcggattta acccccacgc ttattcccgc gcccgtgcgg ccggtccatg gcaatttatg
    41821 aaagggaccg ccaaagtcta tgggctgcgt gtggatagtt atgtggatga acggcgagac
    41881 cctgtgaagt cgaccgtcgc ggctgcgcgc tacctgcggg acctgtacga tttgttcggc
    41941 acctggcctt tggccatggc cgcctacaac gccggtgagg gcaaggtgat gcgagccctc
    42001 catacggcgc aagcggagag cttttcagac attgcaaaaa cacggctgat ccggcgggaa
    42061 acgaaagaat acgtcccccg attcatggcc gcaaccatca tcgccaagaa tccggaccgc
    42121 tatggcttcc cgcagaacga cgtgcggcct caccagtttg aagaagtggt cgtgaagcgc
    42181 cccatccact ttaaagcgat tgccaatgtg acgggcattt cgtaccagga actgaaggta
    42241 ctgaacccgg agttacggcg cgatgccacg cccccggacg atgccgaata tcacctgaag
    42301 gttccggtcg gcaccaggga gaaggtcgaa caattgttgg atcgagcgcc gactcacaaa
    42361 ttcgcgccgc tcccggtccc cgtcagggtc cggcacgtca agcctgagcc ggaatccggc
    42421 cactggtatc gggtgcgcgt aggcgactct ctagagagaa tcgccaagcg attcaacatc
    42481 tccgtcaaaa ccctcaagtc caacaataac ctgacaggtc ccaccatcaa ggtaggaagc
    42541 cgcctggtca tcgcgaacta acgcgaccac ccctcaccgc gtcattctac taagggagcc
    42601 gttacggttt tttcttggag gacgccgcgg cagacttgcc tgaagactta gcaggcgcct
    42661 caggctgggg agcgggcgca gtcacggccg gagcaggtgt agcagccgcc gccggactct
    42721 ccggagactt cttcacttcc agcaccttca ctcgcacggc cgcttcactg gtgaagggcg
    42781 agttaggata attcttcatc aaatcctggt agtgcgcgag ggctccctca ggacgggact
    42841 gcgattcttc caattttgcc agctcgaaga gcacgtggtc tttattcagc gcaccgggga
    42901 tctcaaggat gccggtaaac gccttcaccg cctgatcacg atcccccttc accagatagg
    42961 cgtaggccat ccgctgctgg acaagcccca ggagcgaggt attggaacca tgaagcaaca
    43021 tgaatcgttt gtaggtttcg atccctgcat caacctcatt agccagcacc tgagcattcc
    43081 ctaaatggaa cagggccagg ggagcaacgg ggctccgtgg atattgatcc actacctgct
    43141 tgtagagatt gatggcctgc gcaagctgtt cgtgagattt tttcggatcg tcagccggtc
    43201 gattgagata gtgcagcgtc gcttcctgat cgagttcacg tgctttcagc gtggcctggt
    43261 agtcatacca aatgaccccg gcaaccaccg ccgcggcaac gagcaagact cccagcccaa
    43321 cgagaactgc ccgccgctgt tcctgcaaga caagcagaaa ccgctccact ccggtcagca
    43381 gatgagcttc atcgagcgga tcgacctttg cgggaacctt gattcgataa ctcatgctct
    43441 ctcaatcatt ctctaggcgc agtaaaagaa gcggtcgttt ccgagcgcct cgcgccttat
    43501 actagggagc agtcagcctt gtcaaatatt caagcccttc gcgagtacgg gacaccatgt
    43561 tcaaggaata tcttcaaaaa ttgcaggacc agcacctact ccgtcggctg cgcaccatcg
    43621 cctcctccac ggggccgacg gtcaggctcg acgggcacac cgtgatcctg ctttcctcca
    43681 ataattacct gggcctggcc acccacccag ccgtcgtaga agcggccgtc gaagcgacgc
    43741 gtcaatacgg ggcgggatcc ggggcctcgc gtctcgtctg cggcaccctt acgccccacg
    43801 aagaactcga aacggcactc gcccggttta aagacactga agccacactc accttcgcag
    43861 ccggctatct ggccaatatc agcgccgttc cggcgctgat tggaaaagac gggctcatcc
    43921 tcgccgaccg cctctgccac gcaagtctga tcgacggctg ccgcttgagc ggcgccacat
    43981 ttcgcatcta ccaccaccgg gatatgaatc acctagagca actgcttggc cgccggcccc
    44041 ccggcaaacc gaccctcatc gtcaccgatg ggattttcag tatggatggt gatatcgcac
    44101 cgatgaggga catcgcccta ttggcggaac agtatggtgc cgccgtctat gttgacgatg
    44161 cgcacggcac gggaatcctt ggggaaacag ggcgaggcac cctcgaacat tgcgatgtcg
    44221 aaacgcgcat cccctatcac atgggcacct tgagcaaagc gatcgggagc tccggaggtt
    44281 acctgactgg atcagcggaa ttcgtggcct atctcgtaaa tacctgccgc gccttcacct
    44341 atacaaccgc ccccacgccg gccgccgccg cggccgccac ggcggcgctc cgcgtgatcc
    44401 ggcgagagcc tgagcggcga gccagattgt ggcggaatcg ccatcgcctt gcgcaaggac
    44461 tcagcggcct cggcttccgg ctcgccgctt cggagagccc cattcttccg gtcatcgtcg
    44521 gcgatcccga tcgcgcaatg aacctcgccc acacactgct cacactggga gtctatgccc
    44581 ccgccattcg cccaccgacg gtgccggcgt ccacgagccg gattcgcttc acgatcaccg
    44641 ccgatcacac cgatgagcac atcgaccagg cgctgacggc actcgcacag gcaggtcgca
    44701 cgctcaacat gatctaagca ccaatcgccc tttgcagagc ggctgccagc caccgtttct
    44761 ccctcgtctc ttctgggtat tttcttgctt tctcaccttc ctatggcatg atcgaatcaa
    44821 cctgacgagc catgatcctc tactcatagc gacgctcgtt tatccataca ttctctcact
    44881 gcatggagtg atccgatgat tgatatctcc aaactgttga cctttggcgt ccaacagggc
    44941 gcctcggact gccacatcag cgccggtgaa ccaccgatga tccgcctgca tggcgatctc
    45001 aagaaactcg accatcctcc cctgacccaa gatgaaactc acgcgcttat ctacgacatg
    45061 atgagtgacg cgcaacggaa gaatttcgaa gaacaccggg agtgtgactt ctccttcgac
    45121 ctgggcgaca tcgcccgatt ccgcgtcaac gtctttgtgc aagggcgcgg cttaggcgcc
    45181 gtgttccgga ccattccgac ggagattctt cccctggaaa aactcggcat gccgccgatc
    45241 ctccgacagc tctgcgaccg cgaaaaaggc ctgatcctgg tgaccggccc aaccgggtcc
    45301 gggaaatcca ccacactcgc gggcatgatc gactatctca acaacacctt cgaaggacac
    45361 attctcacga tcgaagatcc ggtcgagttt gttcacaaat ccaaaaaatg cctggtcaac
    45421 cagcgggaac tgggcgtcca cacgctgtcg ttcgccaacg ccctccgcgc cgcactccgc
    45481 gaagaccccg acatcattct cgtcggcgaa atgcgagacc tggatacgat ccagctggcg
    45541 ttgaccgctg ccgaaaccgg acacttggtc tttgcgaccc tgcacacgtc cagcgccccg
    45601 aaaaccatcg accgcatcat cgacgccttc cctccgaacc aacaggccca agtccgcgca
    45661 cagctctcgg aaacgctgga ggcagtcttg acccagacgc tcctgaagaa gaagagtggt
    45721 ggccggattg cggcggtgga aatcatggtc ggtaccacag cggtgcggaa cctcatccgc
    45781 gaaggcaaac tccatcaaat tcccggcatc atgcaggcca gccaaaaaga tgggatgcaa
    45841 accatggaca tggccctggt cgatctggtc acacgagggc tggtgacaaa agctgaagct
    45901 caatcgcgca gcatgaatcc caatctcttt agcgccgctg caggaggcgc cgcgtaaacg
    45961 ggcactcggc gcaaacgaaa cgggtttccc acggcattgg aaagaggcat ttcatggatg
    46021 ttcgcacgct tctcgaagtg atggtcaagc aggaatcgtc ggacctgtat ctcaccgtcg
    46081 atgcgccccc catttatcgc atccatggct cgacacatcg gaccgatgcg ccgccgttca
    46141 cgaacgagca actcgaatcg ctggcgctgg ccctcatgcg aggccagcag cgaggtgagt
    46201 tcgaagaaaa aatggagatg aaccttgccc tttattacaa agacctcggc cgctttcgcg
    46261 tcaacatctt ccgacagaag ggcaacgtcg ggttggtgtt tcgtcatatc aaagcggaga
    46321 tcatgacagt cgaacagcta gacctgccgc ccatcgtcaa agacatcgct atgaccaagc
    46381 ggggtctggt gttggtggtc ggcgcgaccg gctccggaaa atccacgtcc ctcgccgcga
    46441 tgatcgacca ccgcaacacg gtgcatgcgg gccacatcat cagcgtggaa gacccgatcg
    46501 aatttgtcca tcaccacaag aaatcgatcg tcacccagcg cgaagtcggc ttcgacaccc
    46561 attccttcgg gcacgcgttg aaaaataccc tgcgtcaggc accggacgtc attctcatcg
    46621 gtgaaatccg ggatacggaa accatggaag ccgcgatcac gttcgctgaa accggacacc
    46681 tctgcctcgg aacgttgcac tcgaataatg cgaatcaatc gatcgagcgc atcatgaact
    46741 tcttcccggt cgaacggcac gcccagatct atctgcagct ctcattgaac ctgcgggcca
    46801 tcatctctca acgattgatc ccctccctcg acggccgccg cgtgcctgca ctcgaaatca
    46861 tgctggatac accgcgcatc aaggacctca tcaaacgttc tgagatcgat accctgaaag
    46921 aggcgatgga acagggcatc gacgaaggct gccagacatt cgaccacgtg ctgttgcaac
    46981 tctataaggc cgggaagatc agcattgaac aagccttgat caacgccgac agcgccaaca
    47041 acctccgtct gaaaattaaa ctggcggggt tgaaaggcga tgacgcagtg gccgccctcc
    47101 tggacaagag cgaccgggat gagaaggggt tccagattca aggacaactg ggtcagggcg
    47161 ggagcaaaaa gcgctgaggg tcagggcatt gctgctccct gatcctcaag gtctattcct
    47221 ccgcgagcgc acaccacgca attgatcggc acacccgctt aagggcgtcc cgaggggggc
    47281 tcttccaccg cctgttcgag atacgacgcg attttactca atgcagcccc actcagcttc
    47341 cgtccgtacc acgtcggcat cgtatccggt ggaaaccctg gcaccacata ggtccccggg
    47401 gataccactg attccacaat gtactcacga accgtctgcg cctgcccttt atactgtgga
    47461 tcagccaggc gactcgcccc ggtcttcccc agccacagtg gtggaccgac ctgccccttc
    47521 gctcctgcaa tgccgggaat ctgatggcaa accggacagc cggcacggac aaagagctcc
    47581 tgaatcggtt catcccccgt caccaacggc accatgttcg ggtcaatatt ctggacagcc
    47641 ggggttgaat cagatggagg cgagatattg cggatccccg ccagcgcaac caggaacaca
    47701 agccctgccg cgaacgtggc aacggccttg tgctttcggt tcggcggtgg aacagacgat
    47761 gcggcacctg aatcaagcat aggcaggaac caggaaactt acgaaggcct cagtgctcac
    47821 ccctgcctct atttcttggc atccatcgcg cagcgaaatc caatggtgcg gtcctgaaaa
    47881 ccaggctccg ccgagacccg cgcagacgca cttaacgcca caggcaaatc ggcccacgac
    47941 ccgccacgaa tcacccgttt ctcgccatta gcaggacccg tcggattttt gtcgggactc
    48001 ttgccgtaat acgtacgatc gtaccaatcg gctatccact cggccgcatt ccccgccatg
    48061 tgatggaggc cgtacggact ccgccccccg gctttcagcc cgtggcggac actcatccct
    48121 tccaccccac cggccacact caccaacgta atggcttcgc tcacccacac gccgcgattg
    48181 tagttggcaa tgtccacgaa cggctgcatg gggccccacg ggtaccgacg gccatcggtt
    48241 ccacgcgcag ccttctccca ttcagcttcc gtcgggagtc gcttccctgc ccaggcacaa
    48301 taggccgccg catcctccca actcatcccg acagccggac gatcgctgac cgtctccgcc
    48361 gcttcatcat cccaggtcgg cggcgcatca tatctcgccg cctgaagaaa ctttcgatag
    48421 agttgcagcg acacttcata tcgatcgatg acatacgcgt cgagggtcac cacatgttcg
    48481 gggcgctcgg cgggaagccc atcgttgctc cccatcgtaa aaggcccggc cggaaccaac
    48541 accatcggcg caccatcctg ccctgtgatc gcctcagccc cgccggcctg gccatccgcc
    48601 ccagacagtg gctcggccgc tcgcaccgac cagactccga caaacagaat gacggatacc
    48661 acgaaggtca tactgaacag acgcatatac acctctcacc gacggataca cctagacaca
    48721 acccgaccct acccagtcaa agtgtagccg cgagtctgtg caaactcaac tacatgtaaa
    48781 ggagcgtgct ctgaggaggg atttactcaa ctggcggaga cgtgcaaccc ctgcaatcta
    48841 tcccgacact tcgtagaaca actatggggg gacagataca gaaacagatt cggccggcat
    48901 gcgacacatg ccggccgcac gagacaccag gctcatagct ataaatggtt cattacttga
    48961 gctctgcaac ccgccggtcg tggtccttca cagtccgcag cgccgtcaaa tcagaacgct
    49021 ccaggagatt atgcagtact tcctgcttca actgaaccat taacgcctga tgatgcttgg
    49081 aggatggcgc cgtatcctgc aagagattta accagcccgt cgtcgccgcc tcatagcggc
    49141 tgttcggcat ggctgtgtgc agctcctgaa acaccgtaat cgcatcggca cgattctcaa
    49201 agagcgcgac caagccacgc gtgtaatagg catcctcaca ggcggaacct ttgtgacaat
    49261 tcttcatgcg ggaatcctgc gcatgggcga tggcttgaag cgacttcaac tcattcgcat
    49321 caacggggaa gaacgcactg ctcggtaacg tatttgaaga accagcctgc atcgtcgtac
    49381 acccgtggat gccaccaacg agaactgcca atagggcgaa agagataacc gttcggtgcg
    49441 acatcattga cctcctagca aacgcggaaa agttggcggc ctgtagtgac tcggggttca
    49501 tgccttacgc tgatgtcctg actctaccac ccaaaccggg tccggccatt ccacggaggc
    49561 tgtacctctt cggtactggc caaccgcaca ccacaactct ccacaatatc gggctattca
    49621 tacacggtcg gcaggaaaaa ggagggtc
//
LOCUS       DAORRQ010000029        48823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_41_length_48823_cov_38.497006, whole genome shotgun sequence.
ACCESSION   DAORRQ010000029 DAORRQ010000000
VERSION     DAORRQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 48823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48823
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41_length_48823_cov_38.497006"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..938
                     /gene="bluB"
                     /locus_tag="PK224_10700"
     CDS             162..938
                     /gene="bluB"
                     /locus_tag="PK224_10700"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HRB16250.1"
                     /translation="MARDKRERLTRTTAESSEPFSKAEREAVYRAIFERRDVRRNFLP
                     TPIPDEVLARLLNAAHHAGSVGFMQPWDFVVVRTDETKRAVKQLFMDTNAAAALRYKT
                     PRADLYRTLKLEGIEESSLNLCVTCSRQRGGPHVLGRSTVRDTDLYSTCCAIQNLWLA
                     ARAEGIGVGWVSILNHAALKHVLGIPRPVKVLAYLCLGYVSEFAKKPDLEAAGWRARL
                     SVQDLVHYEAWGKRVEGKERLPGCESPRSIQERATRAKRG"
     gene            884..1480
                     /locus_tag="PK224_10705"
     CDS             884..1480
                     /locus_tag="PK224_10705"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRB16251.1"
                     /translation="MRITKVYTRTGDAGQTRLAGGQQVWKDCLRVEAYGTVDELNASV
                     GLVRAMNADAENGSAASTQLEADLRWVQNKLFDVGSILATAPGQTFPNMPEVTAKDVT
                     KLEAMIDRCQEELAPLKEFILPGGGKVSATLHQARTICRRAERICIRLSREEPVAAEL
                     NKYLNRLSDALFVLARWVSKTQGEPEFLWQRESGATAE"
     gene            1485..2021
                     /gene="cobU"
                     /locus_tag="PK224_10710"
     CDS             1485..2021
                     /gene="cobU"
                     /locus_tag="PK224_10710"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRB16252.1"
                     /translation="MKKHPATGSHLILVLGGAASGKSQAALDRAGRRGPKAFVATGQA
                     LDDEMKARIARHRETRPTDWITAEVPRDLAAWFRTEGQAYRTVVVDCLTLWLSNVCGR
                     RIAGQEMVPRVEEVLQAIRGTKARVVLVSNELGFGLVPTSRSARAFRDVAGRVNQQIA
                     AAADEIYFVVSGQSLKLK"
     gene            2038..3093
                     /gene="cobT"
                     /locus_tag="PK224_10715"
     CDS             2038..3093
                     /gene="cobT"
                     /locus_tag="PK224_10715"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRB16253.1"
                     /translation="MTLQEVLTRIQPVDQAIAAQTQARLDRLTKPLGSLGRLEETAVR
                     YATITGEVKPAVPRGVVFTFAADHGVATEGVSAYPREVTPQMVLNFLRGGAGVNVLAR
                     HAGVEVRVVDIGVAYDFGSVSGLIHKKVMAGTNNLLVESAMSRDQAVQALQVGIELAT
                     EAAREGIGLIGTGEMGIGNTTPSAAITAVMTGRPVAEVTGRGTGIDEAGHARKVAVIQ
                     QALERHRPNRADAIDVLAKVGGLEIAGLAGLILGAAAGRVPVVLDGFIAGAAALIAVG
                     LQPLCRDYLIASHRSVERGHQAILEHLRLKPLLDLDLRLGEGTGACLGMSLVQASIKI
                     LTEMATFEEAGVSDREA"
     gene            3176..3982
                     /locus_tag="PK224_10720"
     CDS             3176..3982
                     /locus_tag="PK224_10720"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRB16254.1"
                     /translation="MQHKSRDDSRVSHFTHDVFRPFILAWHFLTAIPISRSHHEPSSA
                     ELATSMAWYSTVGLLIGGLLAAADQGLRLFLTAEVVNVLLIVLLVLLTRGLHQDGLAD
                     TLDGLAGGRTAADRLRIMRDPSVGALGATGLFLSLLLRYAGLLALPQPVRLPVLLCMP
                     ALGRWAMVSVAWASPYARSEGGLAAAFLTHLSWQHVLLSSLVLACALMAGMGVIAATA
                     TIASGALVVVVVRRGCRAWFGGVTGDLLGATNELIEILFLLLIPVLVMAR"
     gene            3979..4935
                     /gene="cbiB"
                     /locus_tag="PK224_10725"
     CDS             3979..4935
                     /gene="cbiB"
                     /locus_tag="PK224_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HRB16255.1"
                     /translation="MTGSELVLAAALDVVAGDPRWLPHPVRGMGVVISWFDNQVRTFC
                     RSDQSLQIAGACLALGLPAAVYAAATWLIAQAAAFSPLLGQLVGIGLAYTTLAGRDLF
                     DHVQAVSRELQQGNLAGAREAVARIVGRDSATLEEPEIVRATVETIAESTADGIIAPL
                     VYLSLGGAPLALAYKAVNTLDSMVGHHDERYEHFGWASARLDDVMNWVPARLTGGFIA
                     LASGLATAQWHRVQDSWYILHRDGDKHPSPNSGMPEAAMAGGLGVQLGGRNYYDGVAH
                     EGELIGDAGTALTPGHIDQATRIMVVAAGLGLFFALLSLWVS"
     gene            4932..6041
                     /gene="cobD"
                     /locus_tag="PK224_10730"
     CDS             4932..6041
                     /gene="cobD"
                     /locus_tag="PK224_10730"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HRB16256.1"
                     /translation="MTRIGHGGDVYAAARELGRDLHRLLDFSASINPLGPSPAAVRTL
                     RTAEVLLGHYPDPACWALRQALAAYWKRPSEEFLVGNGSTELIHLLPRTLGIRHLLLI
                     GPTFSEYAEAMTRAGGRVTMLMADRADGYRPPLESVLATLQKGRQSASGAYPIDAVLL
                     CNPNSPTGQACEAAAVRKCARLVARRGLWCIVDESFAEYANHASILSEPLPPRTVVLR
                     SFTKFYGLPGLRVGYATAKPAVIARIAAQQPPWSVNMLAQRTAVAALKDVRHAGRSLR
                     FMERERTRVQKALTQLPGCTVFPSAANFLLIEVPVGYKATAVVAALRRQGLLIRDCSQ
                     VPGLNDHSVRVAVRPRADNDRLLRALSVLLCKGGA"
     gene            6038..6766
                     /locus_tag="PK224_10735"
     CDS             6038..6766
                     /locus_tag="PK224_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HRB16257.1"
                     /translation="MTMWPDGFTTRYRIAKDTLILDLGTRMRVLSSAPRGGGLRTTRY
                     IVNHQVPSNPVRQATHSKWEHPARYLKRIAVDLGVDPDCVALMTAVPMKQVVTCRDEA
                     DGLWVECFATVGVTNAVRAGEPPPREEADSTVRRAGTINLMVVTNACLAVPALVCAVQ
                     VVTESKTGVLRDHAVPSWTGRSGATGTGTDAVVVACALRGSGPRSDYAGTHTTIGSLI
                     GRVVAECVTQGLARATEWAATHRG"
     gene            6783..8354
                     /locus_tag="PK224_10740"
     CDS             6783..8354
                     /locus_tag="PK224_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRB16258.1"
                     /translation="MPPPSRAKAIAILGTGSDVGKSMITSGLCRLLYRAGFRVAPFKA
                     QNMSLNSFVTPEGGEIGRAQALQAEACGIPPHVDMNPILLKPESDSRSQIIVQGKVFA
                     TREAWAYFARSRDSELFRAVERSYNRLALQYEVMVIEGAGSAAEVNLRDRDLVNWPVV
                     EMADAKVVLVGDIDRGGVFAQIIGTLDLIAPEERDRVCGIVINKFRGDAALFADGVRF
                     LTERTGIPVLGVLPFLRDLALDQEDSLDVDIRRQVAFQRDCINLAVILLPHMSNFTDF
                     NALAGEPDVALRYAATLEEAAGADAILLPGSKTTLADLAYLRGKGFESLLHGHVHRGG
                     EVVGICGGYQMLGRRIADPDAVESGGSGEGLGLLQVTTVLMREKITEQVEGRALYLGG
                     PTAPPVRGYLIHMGRTDRHGHRACFELQGKEPSPAGPRDVTERDDAWLDGAMSDDGLV
                     WGTYIHGLFDEPLCRRAWLNRLRIRKGLPAADMALSQQVNRQRSGAMDRWADHVEQHL
                     DLAPILALLRHGGTV"
     gene            8750..9484
                     /locus_tag="PK224_10745"
     CDS             8750..9484
                     /locus_tag="PK224_10745"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB16259.1"
                     /translation="MAKHIIKRIVMAALTVTCMGAAAPASANTEVETAELLIKLLQVG
                     RGVISEYQATINDASKGDKGFSGDFVSNQVIERFRKATKIDLSRPATVPQAPLFLAMV
                     ESEKEVIDEAQPVINKQGVGFKGIIPAVFARKSGERFYRKTGIRVKLTGTDYRFPGNR
                     PDEFESEVLRIFADTRHPKGQPYSKTTMLDGKPVLRMMDPEYAASTCLSCHGDPKGER
                     DITGAKKEGWHEGDLAGAISIVIPMR"
     gene            9549..9911
                     /locus_tag="PK224_10750"
     CDS             9549..9911
                     /locus_tag="PK224_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB16260.1"
                     /translation="MSKIVVVDDSYAELQLIEGYLKSANHTVVSYPNTDKLEDKLALD
                     KPDLIVLDVVMPGRNGFQACRDLKSDDRFKGIPIVLCTSKGQESDKFWGQQQGANGYV
                     VKPFKAEDLVAAVKRALN"
     gene            9915..10460
                     /locus_tag="PK224_10755"
     CDS             9915..10460
                     /locus_tag="PK224_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRB16261.1"
                     /translation="MSDVPTIQAHSSDSTPAHGPSSPSRSSAASIEGTLRMCVLTLGG
                     ELFAIDLRHVSEVFEVEAVTVVPSMPGLLTGVTNLRGTVITLVDLRGSLGLPVTGTVL
                     PFAVVIRHGSRQVGVLVDHVPEIHTVSREHLLPAMQAGPAGARPCVSAVLRLDERLGG
                     VLEVPQVFAQVDGGSAILSIA"
     gene            10556..12430
                     /locus_tag="PK224_10760"
     CDS             10556..12430
                     /locus_tag="PK224_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRB16262.1"
                     /translation="MIGQGVKNQFQDMKTKTKLLVSFGLVSVIIMIMASVGVLTLRQL
                     STQSQTVYADYTVPLAEFAQMGTALTKHHQILLDIASATKQGDFAQEVTKLPPLKAQI
                     DKVVNNYKSTTLRVSRSGRDEAKDLTLFEPALKKYFQDADGALSAMADSFDRNVLSST
                     QAEQMRALGILALTVNLTPSFENAVKRHNEQVTAIEAVAKDLNDDAQSLANNGTLILV
                     AGGLVAVALGLFVGYLLATFLSRSISHIANVATQAAGGNLQARAKIQSHDELGQMATA
                     FNSMLDRITALVSTEEERDLMQKRLMQFLVLVSEVGKGDLTRRGEVTADMFGNLADGF
                     NLMIARFGQLLKQVREAADRVNKSAGTLRDSAGQMSGTARVQAEESVRTLGAVEQLAA
                     GMRQVATTAGASSDSAKQVLSATERGNVAVQETVRDMQSIRSAVQRMSKQVKGLGDRS
                     LEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESSTQATRE
                     IADLVKVIQTETQDAVVAMEHETQAVEAGSASALRTGDVFAEISDIAKRSSELAQNIA
                     SAASEQTASTEKVGRAIKEFTGGAVATQKQTDSTRLTIEDMAKLAEGLNSSVAQFKLA
                     "
     gene            12483..15872
                     /locus_tag="PK224_10765"
     CDS             12483..15872
                     /locus_tag="PK224_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRB16263.1"
                     /translation="MSADFDRDQLLDIFVAEAGDDMGRFWKALHPDGKAHPEPGDVAE
                     FHTVGHKLKGAALLYGFPALGQLGALLEDTLERVQEIPAARWPEAMQLMREIAASFRG
                     QVEQIGRGGGEDASVVEGFFFMIWVFMSSFSLFSSAASVQRSDQPADEYLIPVLDEEV
                     LSYFSPEAEEYLNTIQTLLQRLDSDLANADTIHQLYRVAHTLKGSAYTVGFEVVGDLS
                     HPIESCMMAVREGAVTIAPHWIAVLRQAVDVIRSLMARDAQSLTRLRQEVPRIRTKLR
                     ELEQGVSQGESAAAAAETRRPEEKSVQEAVPAATSGPESAVHSMSEADSQELSEAYLI
                     PALDSEVMSYFAPEAQEYLESLEADLLRVDKDAGNPDTIHQLFRTAHTLKGSAYTVGF
                     QAIGDLTHHIEDFMGAVREGQLVLLPGHTDVLLRSIDVIRALMRRDVSMVGRTRQRFA
                     NSLQELKQLGQAQPAPDQDHPIVSSIAEGASVEEIREADGQDSAKGVDGKTGEEREVI
                     RVSRDRLERLLNLVGELVIDRGRLEQRLRTLDQLATQVLANKNRLTDAVRTFEDKHTF
                     SFQPSPTSSSEAAPQAYPGVSDFGSLEFDKYDDFNILARRISEVTADITESMSQLSGS
                     IHRAQDDMGSLQQLTLGMRDEIARARMVPIGTPFTRFRRAAREMARATGKEVNLVTSG
                     EHTEIDTGVVERLVDPLVHLVRNAVYHGIEPAGIRRSQGKPAAGTIYLHAAHRGNSVL
                     IEVEDDGAGLDIVKIKAKAVKLGLVRADVADSLPESEIIKFIFLPGFSTAEAVGDQAG
                     RGVGMDVVKRVIETMNGHIEVESVYGQGTKFTMHLPLTLLIATALLVRVGKERYAIPL
                     PSVREVTMSTASTIQQMGDRSVLQIGDEAIEVYPLGSLIRRDAGTIEGATPVVVVRTS
                     TGALGCAVDELLGRQEIVIKSLGGLKPYERSVFGGATIDPEGRVVLVLDVSRLTTREY
                     HEAMSVIQNPASSPMLEGPAQPASPQLPDTQLPLLLIDDSLSIRKFVGRMLESAGYIV
                     ETATDGEEGCRKALVQQYQLIITDLEMPKLNGFEVIQALRARPQTQTTPILVMTTRAG
                     EKHRQMAVSVGASGYIAKPVEERALIQEVRKWTGRESVVKK"
     gene            15920..16492
                     /locus_tag="PK224_10770"
     CDS             15920..16492
                     /locus_tag="PK224_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRB16264.1"
                     /translation="MGLRGHKAAVVSSGLAVRFLVALMGPTHVAFPSHWVRGIVTPAE
                     GGQDGHVTWANASYERTDLARRLTIQAKGVTAETRIVLYANEQRSRSFAVDKVVGLID
                     VERTLIQPLPAQFRGGERERLLGLFVESSYIALIANPFWVLELPSRTNVLDVFALRVS
                     ERRPGEFDSRLRLPSAALEEASAMSAGSAK"
     gene            16509..16985
                     /locus_tag="PK224_10775"
     CDS             16509..16985
                     /locus_tag="PK224_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRB16265.1"
                     /translation="MLRTQLGRQPHKGSKGATEARMQHMVVFSVGGQRLAARTEEIGG
                     VMPWTGSTAVPSDTPFVRALVRQEKGCLPVFDLAAKFKRSLQDGEPLCLVVKHVDGPL
                     AICIDSQVPSLYMVARSAVQYRAGNDPDIAGTCIAGEEELPIINLTTLGVSSNRPA"
     gene            17014..18510
                     /locus_tag="PK224_10780"
     CDS             17014..18510
                     /locus_tag="PK224_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB16266.1"
                     /translation="MPKILIADDSIAVRKVAERLLTEAGMGVTLAANGSEALALLSKD
                     RPDLIVSDVIMPDKSGYEVCAYIRGQANLADLPVLLISGIVNDEVSRQAESCKADGVL
                     KKPFQGSSLKDRVLDLLTKRPQKPAPVPELPQQHVAVEPSIVAEVAVAPAPAIHVPEE
                     LEEARVDTQEHRPSLEPITSSLDDPHAIPATMTRDTMVDAAPAFHAAEPATPAHDHEH
                     HHAILAERDTRIGELEAQLDEERRRSLEQTQQIGHIQRALDEQHEQVAALSSHAHTLD
                     QSLADERAQRTALAEQLDGVSRQMHRIGELETALADEQHRAQQLYQQVSEGARHAARI
                     VELEAHLEAEREAANQLVQQITGLEQVETRVHELETTLAAEREQAEMQRRERVELEQV
                     ADKVPALEEACAKAHAQIAELDAALSAEREAAAALLVQVKQLEATAQRASELDLALVS
                     EQERSMQLEKRAMEAEHMAEQSTRRFEDMARKLGEIAGLASQLGNGKR"
     gene            18546..19517
                     /locus_tag="PK224_10785"
     CDS             18546..19517
                     /locus_tag="PK224_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16267.1"
                     /translation="MGQRQGATVSTEPEQTEDDFQKELIELFGQEAQEWLIQIHSALT
                     ELESQPDTDRHAQLVDAVVRGITSLGGSAATVNLPDVERATFALLPFIDTLKDRTTAT
                     KQDFGTVREQFCLVIASVTRATGITLDLGPEQGAPPQPEPAIDFLTLLNALRMLQDEQ
                     VAKEVPSRSLIPQVMHRLEQEARQGESQIQMTAFQQLLGELQSTDARCLDSLRQALPG
                     IAQNLNRLRVEGLTVLEPGNVLGTCMQQIEQLQGIAKQVNATPLVTFLTGLQNFLALI
                     VQRRIVIASQRVQAVEARILAVMTTIEDWITAGQQERDLMSRLLPAA"
     gene            19717..21789
                     /locus_tag="PK224_10790"
     CDS             19717..21789
                     /locus_tag="PK224_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRB16268.1"
                     /translation="MEQNHYRQAEDALMRVAAAVQRQEPVNLTELTHLAGSIVESIQE
                     TDQLVVEALSSPVGPPLVTNLVNVSILATKVGIGLGYYGAELRRLALAGLLHDIGIFA
                     VPQHLLTKTGRLSSDERALVEQHPRLGYGVIERLGADYAWLAEVVLQAHERGKGQGYP
                     NRLKGREINELAQIIGLVDIFDALVSPRPYRRRLLPHEAIRELLNTERTAFPREIMKG
                     LVEQLSVYPLGTKVRLSSGEEGVVVRITPRYPSRPVLRVTGAQETAVTDPPRFLDLSQ
                     LPQVSVAETVDSPALERVTFATAPMAAEPATPTSASDQFAALLESLDAIAGVIQAAVE
                     QPKAAAQPAQVQSTIEAPDQGPHVRREILGLFVLEAREWLNQIQTALERLDTTSEQGR
                     RAKLTTILWQSVSNLAQSAATVGLTAVEDMATRLLPLLQAAAKHERTVAAHHVASLRE
                     GLLEISKTVQALQALERPPQDHLEAAVAVQQPVTEMPEPMVAEAPAPVEADVPCDAPS
                     TSLPAASILDALRQLHQARGRSLQPVRDVLETVIQRAEQEMARGTGVVDARAIGRLLQ
                     ELDELDERFLAHMQAHIPAVIATLRHVALTSEDRVFPPQALEPIFVEIEALSDAADRV
                     AAANISLFLHGLRTFLRVTAQHKPMVIRERLAAVEERLATLIPLAQQWVDVGRVERAA
                     IFDILPMA"
     gene            22278..22802
                     /locus_tag="PK224_10795"
     CDS             22278..22802
                     /locus_tag="PK224_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16269.1"
                     /translation="MAKLISLKQQEGHDARATAYIKAYMLFPAGILGLISMIGGVGGL
                     GYQLIATDSYTWWTFLQSSGLLLLGGVLGWVQTTYHRWILTNRPEVFAARMRQPTVSK
                     SGKPKRESAASQEQASGSPWAPGAYMVGLAVLLAGSMVSMIYGAVHPIAACFLPWAGF
                     FWAKLFFWKSMLKN"
     gene            complement(22821..23903)
                     /gene="lpxD"
                     /locus_tag="PK224_10800"
     CDS             complement(22821..23903)
                     /gene="lpxD"
                     /locus_tag="PK224_10800"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRB16270.1"
                     /translation="MTTAPRHTPLTLLELHSYVGGELVGSPDATVTGVASLEEAGPGD
                     LAFLTSERNLKSPQTATIGALLVGRRLPDCPSPQLVVDNPAYAFARAAQQFFARPTRM
                     RGIAQGITRGEDVTIGADVSIWPGVTLGDRVSIGARVTLYPGVFIGDDSAIGDDALLY
                     PNVVVREGCRLGARVIVHSGTVIGSDGFGYVQYQGRHQKIPQLGGVLIEDDVELGSNV
                     SVDRATFGNTVIKRGTKIDNLVQIAHNVVVGEHNILVAQVGIAGSTTLGKYVMVGGQA
                     GLADHLQIGDQVMIAAKSGVTRSLEPNQIVSGAPVMPHATFLKAQAVIPQLPELRQRV
                     RELEERLAKLEQIRQPPAKSRKPRRK"
     gene            complement(23908..24249)
                     /locus_tag="PK224_10805"
     CDS             complement(23908..24249)
                     /locus_tag="PK224_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRB16271.1"
                     /translation="MAKTSDVSDKIIQALADYLKRDAASIQTTHHLRDDLGLDSMAVI
                     EMLYRIEEVFNLQIPDQDLVGLTTVGQVIAYVQGRVAPPKAPAKAAAKTPANAAAKPP
                     AAKRSTKSKKA"
     gene            complement(24313..25569)
                     /gene="fabF"
                     /locus_tag="PK224_10810"
     CDS             complement(24313..25569)
                     /gene="fabF"
                     /locus_tag="PK224_10810"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRB16272.1"
                     /translation="MSTRVVITGLGVVSPIGIGVPQFWKAALEGRSGISAITSFDPFP
                     LEGYRSRVAGRILGFSPEQYLPAGQGDRVDRYAQFALVAAKEALADADFRMEREEPHR
                     VGVIVGAGMGGMVMGEREITQLYEQKRPHRVHPNFIPVITLNSASGIVAMAYGAKGPN
                     LTISTACSSSAHALGQAMHAIRAGTADAVIVVGADASITPLVFAGFCSLRALSTKYND
                     APERASRPFDRGRDGFVMGEGAGALIVESLAHAKKRKARMYAEVAGYAATSEAHHMVI
                     PREDGEEVAITMRLALKDAGVTPAQVDYINAHATSTTVGDAVEVKAIRLLFKSRADKL
                     AVSATKSLVGHTLGAAGSLAGIVSALTLTSGQIHPTLNLDDPDPACALAGLSAQPQTR
                     KTKVALINAFGFGSNNAAVVLKSLST"
     gene            complement(25724..26815)
                     /gene="lptG"
                     /locus_tag="PK224_10815"
     CDS             complement(25724..26815)
                     /gene="lptG"
                     /locus_tag="PK224_10815"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRB16273.1"
                     /translation="MNILFRYMLREYVKIFGMCFAGLMTIYLVIDFFEKVRRFLRYDA
                     HALDVLAYFVLKMPAISYQIAPLAVLMATLLTIGLLSRSHEITAMRSCGISLYWITSP
                     FLFLGTVLAMVLFLFSSTVIPLALAKAEQIKTTRIEKRELPVSVKAAQPWVSLGGQTL
                     MNIDTIDPGGAVLRKIRLYRLSPTFELEQIAEAQRAVYAPQGWVLEDGIRRSFRQDGT
                     VSVNSFQTEPLPLAQIPDDFTTWLELDSETMTLPEIRAYVDRLHLGGTILPRLLTDYY
                     GRIAFPCVTFIMVVVGIALSLRRTGVRGSGMAMGIGQAMAVGFFYWSTHSVAIALGRG
                     GALAPMLAGWMANLVFLTFGFYLLLKVRY"
     gene            complement(26820..27905)
                     /locus_tag="PK224_10820"
     CDS             complement(26820..27905)
                     /locus_tag="PK224_10820"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRB16274.1"
                     /translation="MLHTILDRYIFRELFSPFGISLAALCFVMLTKELLRLVELLVTK
                     GIGFLSVLKVFAHLLPSFLVLTLPIAGIIATITAFGRLSLDKELVAMRAAGFSLLRLS
                     QSVFIFAGLVCGLTLVMAQYGQPWSNVNLKKVALNLLRDELVLELDRGVFNEAIPKMT
                     IYVPDAQEGQDNRGIFVADERNPADPRIIVAQQYQVMTDPASSQVALRLMNGVIHSRP
                     QNPEEYQKISFTSYDLKLSLSASLYGAEERTPIDVIRAKLESTGWTDTNALRRLMEYY
                     KDLAFPAASLVFCILGVPVGIVSKRSGSIGGFAVGVLVVIAYYVLNVACEFLVTTLWI
                     SPFAGAWLPNVIFTLVTILWFYRVSRQ"
     gene            28216..28434
                     /locus_tag="PK224_10825"
     CDS             28216..28434
                     /locus_tag="PK224_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRB16275.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDGGDDVFVHYSALQGEGFKTLKE
                     GENVEFDIVQGAKGPQAANVLKDLATAS"
     gene            28611..30530
                     /locus_tag="PK224_10830"
     CDS             28611..30530
                     /locus_tag="PK224_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16276.1"
                     /translation="MAVDRRTVLQNAQLFASKGQYEAAIAEWRKLTTDTPADGTIFNS
                     IGDLQLKRNATPDAVAAFLQAAGAFRSEGSVLKAIAAYKKILKVDPTKIDIYRHLGDL
                     NAERGLLSSAVQDYLTLGKHYFKDGRNKEALEIYRKIISQDPSNLDAQLRVAELCEQE
                     QLVDEAIRVYLQLGRERSAAQQYEQANAMYAAVLRLNPENAEAKEIVTMIEAGGGAPL
                     NLARVAKPSAAAAKPGEGSDLMAEATRRIEEGQYAGAEAMLTQLLSREPGNPEICQLL
                     ARLHLLQGNLQVALGEYRFLAGAALRAQDYVMAESLINDYLKVEPASVPMLELLGELY
                     QEKGDAATAAQHFGRAVEILLEHPEPGMPTLPAELFEKVQALAPGSAIARKLAPAFDP
                     TASSSTEPEVTEPMVMTPEPVAIQLEPEPQPKPAPAPVVAAPAVDMPFRLQDSSATPA
                     APAPPAAPVLAQVAPPPPPVPAPAPPVAQAAPVEPPPAPVPAAQPIAQVDAETQYALG
                     MAYKDMGLLEEAKEEFVSSMKDSGFFVDSCLMLGLCLKEQNRFDQAIQLLEKLIADSR
                     CRGGNAQLVRYELAMLYEKTGARDRALAMYQSIPSFHDVPRRVEALRTLGTNGVAHPE
                     LPASSQTSPLPLAGH"
     gene            complement(30586..31557)
                     /locus_tag="PK224_10835"
     CDS             complement(30586..31557)
                     /locus_tag="PK224_10835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRB16277.1"
                     /translation="MSPPILYISRLLPDPVMAAARQQFQLFNEPRDIAPSWETFKAGL
                     READAAICTLTDRVDTALLAEPTRLKILANYAVGYNNIDLAAATAKGIVVTNTPDVLT
                     DSTADLTWALILAVARRVAEGDAYVRAGAWPGWAPTQMLGTDVSGKMLGIVGMGRIGQ
                     AVAKRAAGFNMRICYSSRTPHAPGQGSSQWEQRALPDLLREADFVTLHVPLSPETHHL
                     IGPRQLALMKPTAFLINTSRGPVVDEGALVDALLRRRLAGAGLDVFEQEPVFHPSFRD
                     LRQVVLLPHLGSATLATRVRMGMICLENVAAVCAGRPAPNQVNRTQA"
     gene            31665..34505
                     /locus_tag="PK224_10840"
     CDS             31665..34505
                     /locus_tag="PK224_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HRB16278.1"
                     /translation="MTLILPNTSSVVVSGLRQLLGQAKVRNDVPTLTAYAVDASIYRM
                     QPQAVVLAEHEGDIDTVVRFAVERGIPLTPRAAGTNLTGSAIGSGIILDISKMNRILE
                     VNEEERWARVQPGIVLAELNKQLGRRNLLFGPDPSSGDMCKLGGMLANNSAGPHTLIY
                     GAVKDNVQAMRVCLPSGSWLSASSMNLNDPGLDQTLVAHPSLKPILDLVQRQHSLIDS
                     KKPTVSKNSCGYNLFGLADGLSRGVFDLPKLFVGSEGTLGVVSEATLRLVPKPQGTLT
                     ALIHFRRLEEVGEAVPHLLSLRPSALEVMDANTLNLIGRSAHGIPADAAATLLAELDS
                     SEGEGDLRERADQMAAICGRYQLCGDLTIAYDKEQRDQLWKARKALYPTLYRFDPRKK
                     PINFVDDVVVPATRISELIRYLETFFEGQHVPVAIFGHIGNGNAHITPLLDVNDRQDF
                     DKMVRAYHEIHGAVLSRFDGSICGEHGDGRVRAEYVRKMFGEELYQLFVQVKQTLDPA
                     NVMNPGIKISETPFTEHIDYQRLSKSCATCAKCNSVCPVYDVFQSEDMSSRGWFEIVT
                     AKDYSYLDSKRVVEACLNCKSCRTICPAGVDVSELILQRRAENPNQGSRWLFALQAKL
                     PVFEAILKLLARTQSWWDRPAPRALLERLAAPVMKRIASTAKLPADMVLPKLATTHLR
                     NRYPELTQPSAKKAGPRVAYFHGCAANYFDDGVGDAVISVLRKHHVEPELPPQRCSGT
                     PIETYGHVDLVKDNARFNLQSLAGYDQVVTGCASCTLMLKEYKKFFESGPEQQAADVL
                     AKKVVHITEFVARSGQHPPMGTPEGQGGKVTYHSSCHLRAAGVTKEPRQLLKQLPGSD
                     YVEMTDADRCAGGAGTYIVKDYDTSQKVFDRKRRNIEQSGAETVATSCPACMIQLSNG
                     MRGRVAVKHVAQLLNDAYEAGAATETRPGAGQ"
     gene            34502..34744
                     /locus_tag="PK224_10845"
     CDS             34502..34744
                     /locus_tag="PK224_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16279.1"
                     /translation="MTFRKVDIIFVVLALLVVGGVALLPSPRDRNPKVPANSGHQAVT
                     MEKDCLACHVPTGARPLPVQHPKRQDCLHCHARAQG"
     gene            34902..35180
                     /locus_tag="PK224_10850"
     CDS             34902..35180
                     /locus_tag="PK224_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16280.1"
                     /translation="MVKVLILGPTLQQRVTEPELDVEVEGSVPIKLLIEGNADLMDAL
                     APFVVRGELLVTVNRKVGSLDSVVKDGDVLKISHQSRASYDGTTDIPT"
     gene            35359..36342
                     /locus_tag="PK224_10855"
     CDS             35359..36342
                     /locus_tag="PK224_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRB16281.1"
                     /translation="MARNDQAVRLLVVLKQLEASRRGLTLEQLAESLAPGSTRHPRTL
                     RRDLAALEEAGYPLVTERINGQTCWRLMEGFRNVPGLRFSPSELMALTFSRRLITPLE
                     GTELHTSLQSALGKAAAALPPQGVALVQQLDGTFSVGLGPHKRYRQHRETIDRLTRAI
                     ADATTVQIRYDSASRGRTTRREVDPYRLWYATGGLYLIAYCHLRREPRMFAVERIKSV
                     TPTDHPYQMPLHFDLDAFVQDALTVMRGPRIEVELEFNKATAAWVKDRVWHATQETKR
                     TKGGGLRMTLSVADTRELVGWVLSFGSGVKVLKPESLHQAVVEEARRISQA"
     gene            36521..36754
                     /locus_tag="PK224_10860"
     CDS             36521..36754
                     /locus_tag="PK224_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16282.1"
                     /translation="MATVNFSVPEDVKKRFDRAFAGQNKSRVIADLMAQAVEEQLMLK
                     RRSDAIDSLLSRRRTKRPVAARAIRQARESGRQ"
     gene            36751..37179
                     /locus_tag="PK224_10865"
     CDS             36751..37179
                     /locus_tag="PK224_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRB16283.1"
                     /translation="MTRVVVDASVVVKWLLPQREDEADVEQALMLLDGLRSGRVKIFQ
                     PVHWLAETAAVLVRLSPETAAADVADLYAMSLPIESGPEVYLTACELAHTLDHHVFDT
                     LYHAVALTLPDTVLVTADARYERTARRRGAIMLLRDVVSV"
     gene            37230..37358
                     /locus_tag="PK224_10870"
     CDS             37230..37358
                     /locus_tag="PK224_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRB16284.1"
                     /translation="MADSRKVVGWVLSFGSGVKVLKPDSLREAVSQEAQRIAEQGE"
     gene            complement(37414..37728)
                     /locus_tag="PK224_10875"
     CDS             complement(37414..37728)
                     /locus_tag="PK224_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRB16285.1"
                     /translation="MSIPNKRARKVRPTHPGAMLREDFLPEYGLTVSGCARAIRVSRQ
                     TLNELLQERRAVSPEMALRLSRLFGNSPEFWLNAQRAVDLWEAARNIKAQINHISPLP
                     AA"
     gene            complement(37756..38022)
                     /locus_tag="PK224_10880"
                     /pseudo
     CDS             complement(37756..38022)
                     /locus_tag="PK224_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            38660..39001
                     /locus_tag="PK224_10885"
     CDS             38660..39001
                     /locus_tag="PK224_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB16286.1"
                     /translation="MKNTKRAKLEAAGWAVGSVKEFLGLSEAESALIDMKLALSRSLR
                     DRRNKQGLSQVQLAERLQSSQSRVAKMEAGDPSVSMDLLVSSLLLLGASSADLAKAIS
                     GEGRGKIWRAA"
     gene            39257..39439
                     /locus_tag="PK224_10890"
     CDS             39257..39439
                     /locus_tag="PK224_10890"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRB16287.1"
                     /translation="MSIRELLRSKRAEILEIAARHGARKVRVFGSVARGTARPSSDID
                     FLVEMEEGRSLLTMRH"
     gene            39646..39906
                     /locus_tag="PK224_10895"
     CDS             39646..39906
                     /locus_tag="PK224_10895"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRB16288.1"
                     /translation="MPELCRFLGMIITMYYDDHAPPHFHIRYGDYKAIMGIDSLMLLD
                     GYLPPRALGLVAEWGALHRDELWEDWSLAEQRAPLKKIKPLE"
     gene            39912..40190
                     /locus_tag="PK224_10900"
     CDS             39912..40190
                     /locus_tag="PK224_10900"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRB16289.1"
                     /translation="MLKDLVEAAPLDGYRVRLRFEDGIEGELDLATIIHFEGVFAPLK
                     DLVRFRELAVHPDLGTIYWPNGADLDPVVLYTRITGAPLPKYEAKAIH"
     gene            40418..40786
                     /locus_tag="PK224_10905"
     CDS             40418..40786
                     /locus_tag="PK224_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16290.1"
                     /translation="MMRHAALIGAFLLMAMTGTGRAEPGFDEKYQRDFNIFNPINQYQ
                     PGNPLNPINAYDPANPFNPINRFDPGNPANPINQFNPNNPFNPVNRYRPENPLNPINE
                     FNPNVPFAPLDGGGGMGKRR"
     gene            40975..43668
                     /locus_tag="PK224_10910"
     CDS             40975..43668
                     /locus_tag="PK224_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRB16291.1"
                     /translation="MPKKTKPVTQKNQTAPYKHPEAKSLMRPEVGTQAQFKKKKPAKT
                     YRYDSSLSPGLDWDATNPAREEGEALIKKVLDAKTLEEAKAAASKLKSLSMPFLNWAG
                     KAERLSFDVPTLPLFIHERLSTKAIIETLAGHKTDKQEDMFALFGDPQHSITDQVLKS
                     YEYQDDWTNRMVLGDSLVVMNSLLHYEGLGGQVQMIYMDPPYGVKFGSNFQPFVRKRD
                     VSHNDDEDMTREPEMVQAYRDTWELGLHSYLTYLRDRLLLARDLLTPSGSIFVQISDE
                     NLHHVREVMDEVFGKENGCCVIAFKKTSSVSSPMARVNVLGTVSDYLLWYAKDIERVK
                     YRQLYQTKNLESDVAGVYSRIASPDGKEMRRMSPAELSGEVSIPKGWKVFRIDNMTSQ
                     GHSEVLSQPFIMKGQSYFPGNNRHWATTSENLKRLEAMGRLVPAGKSLGYVRFLNDFP
                     VYPIVNIWTDTGTGSFTEDKLYVVQTATKVVERCLLMTSDPGDLVMDPTCGSGTTAYV
                     AERWGRRWITADTSRVPLALARQRLLTATFPWYELKEEARGPAGGFTYKRKQNKSEEE
                     VGGIVPHITLKSIANNEPPAEEVLVDRPEVENGITRVTGAFCFEATIPTPVDWEGDGI
                     EDSGVVPGEAYGSFVDRMLEVLRKSPVIRLEGNKTVTFKNVRPPAKSLSLSAEALVVN
                     GQEKAVAMVFGPENGAVSEKLVYEAAREAHAKNYTHLYVIGFAIQPNARTLVEKCADV
                     MGVPATYVQATPDLMMGDLLKNMRSSQIFSVCGQPEIKVIRTKDKQYQVELLGLDVFD
                     PITMDVTHRSGNDVPAWLLDTDYNDLCFHVSQAFFPRTNAWDNLKKALKGEYEESVWD
                     HLSGTISAPFEAGEHKQIAVKVIDDRGNELLVVKKLNGTSR"
     gene            43665..44183
                     /locus_tag="PK224_10915"
     CDS             43665..44183
                     /locus_tag="PK224_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB16292.1"
                     /translation="MIERVTIRRFKRFQDVRFDLPGHIVLAGPNNCGKTTMLQAIAAW
                     DLALTRWKQLHDFQRHRGFYTKAPIARQAFTAVPLRAFDLLWNNRSYSGSIEIEIRHS
                     KGWWVTMELMADSTEQVYVRPKPDADPEMVRLAELKTVFVPPMTGLRALPSQSIGDRR
                     WINSSDRASPVK"
     gene            44144..45325
                     /locus_tag="PK224_10920"
     CDS             44144..45325
                     /locus_tag="PK224_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB16293.1"
                     /translation="MDQLLGQGKPGEVIRNLLAEAFQSSAWSKLEDSIRRLFNYEILP
                     PDSTGADIIAEYRFQPDGPRLDIASAGSGFQQVLMLLTFLHARPGATLLLDEPDAHLH
                     VILQDAIYGELRSVAAKTSSQLIISTHSEVIINSVDSRELCMLINHPRLLESTVERSR
                     LTDSLGILTHTDIMIAQDAPGILYVEGYTDVNLLIEWAKTLGHPAYQTLTTKLFWRPS
                     IWEPRPWAHGVKAEDHYQAIQLVRPGLPGLILLDGDDNSRVPETPITGQGLQRLRWKR
                     YEIESYLIHPATLERFVEKEVGTGAQSVEAKKDMNAYLEGTFTPAFLQDPLKPNPLVD
                     AYLQQRKARTDVLPPILAAAGLHAFPYTRFNEIASVMLPEEIHPEVKEKLDAIQRAFG
                     L"
     gene            45322..48429
                     /locus_tag="PK224_10925"
     CDS             45322..48429
                     /locus_tag="PK224_10925"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRB16294.1"
                     /translation="MSAYEVPQPILNAPFEEPKEHWHIVEGEEPEQRPGRRPAMYFYR
                     DPKVKPEKEYGTVAGTAIELKLVNRIRAQVKKWRADGYPGVTRTTLELLQWWRREGRA
                     QRLFFAQLDAVETIIFLCEARADFRQGIEVPQEELSEDKRAQGFIGFRRYASKMATGS
                     GKTTVMGMLAAWSILNKVNDRGDARFSDVVLVVCPNVTIKSRLRELDPEAGEVSLYRT
                     RDLVPSHLMPLLTRGRVLVTNWHVFEPQGIQTGGTGSRVNKAGVELRTKETLIIGPKT
                     TTARGKRYLTLEDFERQVAAGMLTVLGEERENDGSLKKVSVESRRYVESDTALVNRIL
                     GREVGGKQNILVMNDEAHHAYRIVRDVRDEDEEDLFGEDEEAEDFFKEATVWVDGLDR
                     IQKLRGINLCLDLSATPYFLGRVGQDANRPFPWVVSDFGLIDAIESGLVKIPQLAVRD
                     TTGKEIPGYFNIWHWILPQLTPAERGGKKASPKPEAILKYAHHPIAMLGGLWEKERED
                     WIANREDPRPPVFILVCKNTQIARVLYEWLAEDTAPTGIPPVKIEGFRNNGLQHTIRV
                     DSKVVRESDSGESKSDEVRWMRFMLDTVGKQTWPTDRVGRPIFPEGFKELTDKLGRPD
                     HPPGRDVRCIVSVGMLTEGWDCNTVTHIIGLRPFMSQLLCEQVVGRGLRRASYELGPD
                     DRLTEEVAKVFGVPFEVIPFKSSPQGQPKPRVKRFHVHAIPSKAQYQIKFPRVEGYTQ
                     AIRNRVTVDWDSVPSLVLEPGRIPPEVEVKGLHVNTKGKLSLSGPGRIDEVNLDEFRA
                     RRRLQELVYDLAKTLTHSYVSQKNCGVAAHVLFPQVATIIHRYLEKKIDVRSPANIKD
                     LFLAPYYGWVVERLLEAIRPDTSQGESPEVPRYETSRGSGSTGEVDFWTSRDVREVGK
                     SHLNYVVADTAQWEQSAAYYLDKHPKVDAFVKNSGLGFAIPYLDNGQMHDYVPDFIVR
                     LKHAPSHHLILEIKGFDPREDVKRAAAERWVAAVNADGAHGTWGYAIAKKVADIRPLL
                     EEHSR"
BASE COUNT        10067 a        13848 c        15118 g         9790 t
ORIGIN      
        1 gttcgagtgt gtcgggtatc aatgctggtg cgggaagatc ggtattaccg aggagcagat
       61 ggattggatt gcggcacgtt ttaaggactg cctttgcccc gtctgtttgc agcaggtggc
      121 ggatggaata ctcggaccgc aggcttcgaa tggaatgcct catggcacgt gacaaacgag
      181 agcggttgac tcgcacaacg gccgagtctt cagaaccgtt ttccaaggct gaacgagagg
      241 cggtctatcg ggctattttc gagcgccggg acgtgcgcag aaactttctg ccgacgccga
      301 ttcccgacga agtgctcgca cgcttgctga acgccgcgca tcatgccgga tcggtggggt
      361 tcatgcagcc ctgggacttt gtggtggtcc gtacagatga gacaaagcgt gcggtgaagc
      421 agctgtttat ggacacgaat gcggctgcgg cgctccggta caaaacacct cgtgcggatc
      481 tgtatcgaac gctcaagctg gaaggtattg aagaatcgtc gctcaatctc tgtgtgacct
      541 gcagccgcca acgaggcggc ccacatgtgt taggtcgttc gacggtgcga gatacggatc
      601 tctatagcac ctgttgtgcc atccagaatc tgtggctggc tgcacgggcc gaagggatcg
      661 gggtcggatg ggtcagtatt ttgaatcacg cggcgctgaa acatgtgctg ggtattccga
      721 ggccggtcaa ggtgctggcc tacctctgtc tcggatatgt gtcggagttt gcgaagaagc
      781 cggaccttga agccgccggg tggcgagcca ggctttctgt gcaagatttg gtgcactatg
      841 aggcatgggg taagagagtc gaagggaagg agagactacc gggatgcgaa tcaccaaggt
      901 ctatacaaga acgggcgacg cgggccaaac gaggttagcc ggcggacaac aggtctggaa
      961 agactgtctg cgggtcgagg cctacggcac agtcgatgag ttgaatgcgt ccgtcggatt
     1021 ggtgcgggcg atgaacgccg acgccgagaa tggctctgcc gcgtcgacgc aattggaggc
     1081 ggatttgcgc tgggtgcaga acaagctctt cgatgtcggg agtattctgg ccacggcgcc
     1141 gggtcagacc tttcccaaca tgccggaggt gacggccaag gatgtgacga aactcgaagc
     1201 gatgatcgat cgatgccagg aggagcttgc cccgctgaag gagttcattc tacccggtgg
     1261 cggtaaggtc tcggcgacgc tccaccaagc gcgaacgatc tgccgccgcg cggaacgaat
     1321 ctgtatccgg ttgagccgcg aggaaccggt ggcagccgag ctcaataagt atctgaaccg
     1381 gttgagcgac gcgctgttcg tcctggcccg ctgggtgtcg aagacccagg gcgaaccgga
     1441 atttttgtgg caacgcgaat ccggcgcaac cgccgagtaa gccgatgaag aagcacccgg
     1501 ccacaggatc ccatctcatt ctggtgcttg gcggcgccgc ctcgggaaaa agtcaggcag
     1561 ccctcgatcg ggcgggccgt cgaggcccga aggcctttgt cgcgaccggg caggcgctgg
     1621 acgatgaaat gaaggcgcgt attgcgcggc accgcgagac ccgtcccacc gactggatca
     1681 ctgccgaggt gccgcgtgac ctggctgcct ggtttcgaac ggaaggacag gcctatcgta
     1741 ccgtcgtggt ggattgtctg acgctctggt tgagcaacgt gtgcgggcgt cgcattgccg
     1801 ggcaggagat ggtccctcgt gtggaagagg tgctgcaggc tattcgcggc accaaggcgc
     1861 gggtcgtgtt ggtgagcaac gaattgggct tcgggctggt accgacgagt cgcagtgcgc
     1921 gcgcgtttcg cgatgtcgcc ggtcgggtca accaacagat cgcggcagcg gccgacgaaa
     1981 tctactttgt cgtcagcggc cagtcgctca aactcaaatg acaggggaga agacccgatg
     2041 acgctccagg aagtgttgac acgcattcaa ccggtggatc aggcgatcgc agcgcagacg
     2101 caagcgcgcc tggatcggtt gaccaaacct ctcggtagtc tggggcggct ggaagaaacc
     2161 gcggtgcgtt atgccacgat cacgggcgaa gtgaaaccgg ctgtgccgcg cggagtcgtg
     2221 tttacgtttg cggccgacca tggtgtggcg actgaagggg tgagcgccta tccgcgcgaa
     2281 gtcacgccgc aaatggtgct gaatttcctg cgtggcggcg ccggcgtgaa tgtgctcgcg
     2341 cgtcacgccg gagtcgaggt gcgggtggtg gatatcgggg tcgcgtacga ctttgggtcg
     2401 gtctccggcc tgattcacaa gaaggtgatg gcgggaacca acaatctcct ggtggaatcc
     2461 gccatgagtc gtgaccaggc ggtgcaagca ttgcaggtcg ggatcgagtt ggctacggag
     2521 gctgcgcgtg aggggatcgg tctgatcggg accggcgaga tgggcatcgg caacacgacg
     2581 ccgagtgcag cgatcacagc agtcatgacc gggcggccgg tggctgaggt cacgggtcga
     2641 ggaaccggca tcgatgaggc gggccatgcc cgcaaagttg ccgtcatcca acaggcactc
     2701 gaacggcatc gtccaaaccg ggccgatgcc atcgatgtgc tcgcgaaggt cggcggtctg
     2761 gagattgccg gtttggccgg actcattctt ggtgcggcgg caggacgggt ccctgtggtg
     2821 ttggatgggt tcattgccgg cgctgcggcg ttgatcgcgg tggggttgca gccgctctgc
     2881 cgggactatc tgatcgcctc gcaccgttcg gtggaacgtg gtcatcaggc gattctggag
     2941 catctgcgct tgaagccgct gctcgatctc gatctgcggt taggcgaagg gactggagcc
     3001 tgcctcggga tgagcctcgt gcaggcgtcg atcaagattc tcaccgagat ggcgacgttc
     3061 gaagaagcgg gtgtctcgga tcgtgaggcg tgacaggtga aacgtgaagc gtgaaatgcc
     3121 aagcgaggga accgtagaca cggcacgttc gcatccggca gaatccgggc cccgcatgca
     3181 gcataagagt cgcgatgatt ctcgcgtttc acattttacg catgacgttt tccgcccgtt
     3241 catcctggct tggcatttcc tcacggcgat tccgatcagc cggagccacc atgagccgtc
     3301 gtccgcggag ttggctacgt cgatggcctg gtactccacg gtcgggcttc tgatcggcgg
     3361 attactcgcg gcggctgacc agggcctgcg cctgttcttg acggccgagg tggtgaatgt
     3421 cctgctgatt gtcctgctgg tgctcctgac acgcgggttg catcaagacg ggctcgcaga
     3481 caccctggat gggttggccg gcggacgcac ggcggccgat cggcttcgca tcatgcgcga
     3541 tccgagcgtc ggtgcactcg gcgcgaccgg cctttttctc tcgctgctgt tgcggtatgc
     3601 cggattgctc gccctccctc aaccggttcg tcttcccgtg ctgctctgta tgccggcgct
     3661 gggccgttgg gcgatggtct cagtcgcctg ggcgtctccc tatgccagga gcgaaggcgg
     3721 gctagcggcc gcgtttttga ctcatctctc ctggcagcat gtgttgcttt cgagtctcgt
     3781 gttggcctgt gcgctgatgg ccggaatggg agtgattgcg gcaacggcga ctatcgcatc
     3841 gggagcgttg gtggtggtcg tggttcggcg cggttgtcgg gcatggttcg gcggagtgac
     3901 gggagatctg ttgggtgcca cgaatgaatt gatcgaaatt ctcttccttt tgttgatccc
     3961 ggtgttggtg atggcgcgat gaccggcagc gagttggtac tggcggcggc gctggatgtc
     4021 gtggcgggag atccccgttg gttgccccat ccggtgcggg gcatgggcgt ggtcatttcc
     4081 tggttcgata atcaggtcag aacattctgc cggagtgatc aatccttaca aatcgccggg
     4141 gcctgtctcg cgctggggtt accggcggcg gtctatgcgg cggccacctg gctgattgcg
     4201 caggcggctg cattttctcc gctcctcgga caactggtcg gcatcggcct ggcctatacc
     4261 acactggcgg gccgggacct cttcgatcat gtccaggcag tcagtcgtga gctgcagcag
     4321 gggaatcttg ccggagcgcg tgaggccgtg gccaggatcg tcggccggga cagcgccacg
     4381 ttggaggaac cggaaatcgt gcgcgccacg gtggagacga tcgccgaaag caccgccgac
     4441 ggcattattg cgccgctcgt ctacctctcc cttggcggcg cgccgctcgc gctggcctac
     4501 aaggcggtga atacgctcga ttcgatggtg ggacaccacg atgagcggta tgaacatttc
     4561 ggctgggcct ctgcccgatt ggacgatgtg atgaattggg tgcctgcccg gctgactggc
     4621 ggcttcatcg cgctggcttc gggtctggcg accgctcagt ggcatcgtgt gcaggacagt
     4681 tggtacatcc tgcatcggga cggcgacaaa catcccagcc cgaacagcgg catgccggaa
     4741 gcggccatgg ccggtggatt gggcgtgcaa ctcggcgggc ggaattatta cgacggtgtg
     4801 gctcacgagg gtgaattgat cggtgatgcc ggcaccgcgt tgacgcccgg gcacatcgat
     4861 caggccactc gcattatggt ggtcgctgcc ggactggggc tcttctttgc actgctctcc
     4921 ctatgggtat catgacgcgc atcggtcatg gcggggatgt atatgcggcg gcccgtgagt
     4981 tagggcgaga tctccatcga ctcctggatt tcagcgccag catcaatccc ttgggaccgt
     5041 ctcccgccgc agtgcgaacc ctgcggactg ccgaggtgtt gctcggtcat tatccggacc
     5101 cggcttgttg ggcactgcgg caggcgctgg ccgcgtattg gaaacgcccg agtgaggagt
     5161 ttctggtcgg caacggatcg accgagttga tccatctcct tcccaggaca ttggggatca
     5221 gacatctcct gctgatcggc ccgacctttt ccgaatatgc tgaagcgatg acacgcgccg
     5281 ggggacgggt caccatgctg atggcggatc gtgccgacgg atatcgtcct ccgctggagt
     5341 cggtcctcgc aacgttgcag aaggggcggc agtctgcgag cggcgcctat cccattgatg
     5401 cagtactcct ctgcaatccc aatagcccga ccggtcaggc ctgcgaggct gccgcggtaa
     5461 ggaagtgtgc gcgtctggtg gctcgccggg gactctggtg tatcgtggat gaatcctttg
     5521 cggaatatgc gaatcatgcg tcgatcttgt cagagcctct tcccccacgg accgtcgtgc
     5581 tgcgcagctt taccaagttt tatgggttgc ccggactgcg tgtgggctat gccacggcga
     5641 agcctgctgt gattgccaga atcgcggcgc agcagccgcc ctggtcggtg aacatgttgg
     5701 cgcagcgaac tgcggtcgcc gccttgaaag atgtgcgaca cgctgggcga agccttcgtt
     5761 tcatggaacg ggagcgaacc cgcgttcaga aggcactgac ccagctgccc ggttgcacgg
     5821 tctttccctc tgccgccaat tttctgctga tagaggtgcc ggtcgggtac aaagcgaccg
     5881 ccgtggtggc cgcgcttcgc cggcagggac tcctgataag ggattgttcg caagtgcctg
     5941 gattgaatga ccattcggtg cgcgtcgcag tgcgtcccag ggccgacaat gatcgcctcc
     6001 tgcgagccct gtcggttctc ctttgcaagg gtggcgcatg acgatgtggc cggatggttt
     6061 caccactcgc taccgaatcg ccaaggacac gctcatcctc gatctgggaa cccgcatgcg
     6121 agtcttgtcg tccgctccaa gagggggagg gcttcgaacg acgcgctaca tagtgaatca
     6181 tcaggtgccc tccaatcctg ttcggcaggc gactcactcg aagtgggaac atcctgcccg
     6241 ttacctcaag cgaatcgcgg tggatttggg agtggatcct gattgtgtcg ccctgatgac
     6301 ggcagttccg atgaaacagg ttgtgacgtg ccgtgatgag gcagatggcc tctgggtcga
     6361 gtgttttgcc accgtcggtg tgaccaacgc ggtgcgggca ggtgagccgc cgccgcgaga
     6421 agaagctgac agcacggtcc gtcgagccgg gacgatcaat ctgatggtgg tcaccaatgc
     6481 ctgcctcgcc gtgccggccc tggtctgtgc ggtgcaggtg gtgacggaga gcaagaccgg
     6541 agtgctgcgt gaccatgcgg tgcccagctg gaccggtcgt tccggcgcga ccggcacggg
     6601 gacggacgcc gtggttgtcg cctgtgccct gcgggggagc ggtccccggt cggactatgc
     6661 cggaacccac actacgatcg gatccttgat cgggcgggtc gttgccgagt gcgtcacgca
     6721 aggtctggcg cgcgcgactg agtgggcggc gacacatcgc ggctaaacgg ttgtcaattc
     6781 ctatgcctcc tccttctcgt gctaaagcca ttgcgattct cgggaccggc tcagacgtcg
     6841 ggaaaagcat gatcacgtcc gggctctgcc ggctgttgta tcgcgccggg ttccgcgtgg
     6901 cgcccttcaa agcgcagaat atgtcgttga actcctttgt cacgccggag ggcggagaga
     6961 tcgggcgcgc tcaggccttg caagccgaag cctgtggaat tcccccccat gtggatatga
     7021 atcccattct gctcaagccg gaatccgatt ccaggtctca gatcatcgtg cagggaaaag
     7081 tatttgccac gcgcgaggcc tgggcctatt ttgctcggag ccgggactct gaattgtttc
     7141 gggcagtgga acgcagttac aaccgcctcg ccttgcagta tgaggtcatg gtcatcgaag
     7201 gcgccggcag cgctgcggag gtcaatctgc gagatcgcga tctggtgaac tggccggtcg
     7261 tcgagatggc cgatgccaag gtggtgttgg tcggggatat cgatcgcggc ggcgtgttcg
     7321 cacagattat cggcacgctc gatctcatcg cgccggagga gcgtgaccgg gtctgcggga
     7381 tcgtcatcaa caagttccgg ggagacgcgg cgctctttgc cgacggcgtc cgttttttga
     7441 cggagcggac agggattcct gtcctgggcg tcttgccgtt tttgcgcgac ctggcactcg
     7501 atcaagagga cagtctggac gtcgatatcc gacgacaggt cgcgtttcag cgtgactgta
     7561 tcaatctggc ggtgatcctc ctgccccaca tgagcaactt caccgatttc aatgcgctgg
     7621 ccggtgaacc ggatgtggcg ttgcgatatg cggcgaccct cgaagaagcg gccggggctg
     7681 atgcgattct gcttcccggt tcaaaaacca cattggcgga cctcgcctat ctgcgaggca
     7741 aggggttcga atcactccta catggtcatg tccaccgcgg gggagaagtg gtcggcattt
     7801 gcggcgggta tcaaatgttg gggcgccgga tcgccgatcc ggatgcggtc gaatcaggcg
     7861 ggagcggcga ggggctcggt ctgctgcagg tgacgacggt gttgatgcgg gagaaaatca
     7921 ccgagcaggt cgaagggcgt gccttgtatc tcggtgggcc gacggcgcca ccggttcgag
     7981 ggtacttgat tcacatggga cggacggatc gccatggaca tcgagcttgc ttcgaactgc
     8041 agggcaaaga accgtcgccc gcaggaccac gcgacgtcac cgaacgggat gatgcgtggc
     8101 tcgacggcgc gatgagcgat gacggtctgg tctgggggac ctacatccat ggcctgttcg
     8161 acgagccact ctgtcgacgt gcatggttga accggttgcg tatcagaaaa gggcttccag
     8221 ccgccgacat ggccttgtcg caacaggtga accgacagcg atccggtgcg atggatcgat
     8281 gggccgatca cgtcgaacaa catcttgatc tcgccccgat cctggcgctc cttcgtcacg
     8341 gtgggacggt ctaggccagc cgcaatcctc ttttcgtcga agttctgaag ggactttgac
     8401 gcttccctcg aaaagcgacc aaactttggc gctccgactt ccatcgtgaa caattacgca
     8461 cccatgtcag cctagttgac catagtgggt atagtagacc atccacacat aatcatccgc
     8521 aaaatcgccg acttcatcgg ttcgaattct ttccgctccg caatcctaac taattgtttt
     8581 gtttggcacc gatcgtgcac aacgtctgcc tgggctacag ggtcctgacg tggttccatg
     8641 cgttgatgaa attgtcaacg aagaattttg cgctatcttt gacaagggat tggctgactt
     8701 aggggaattg ggttgaccgg gcgctacgag gcaggcagga ggagacggca tggcgaaaca
     8761 tatcatcaag agaatcgtga tggctgcatt gaccgtgacc tgcatgggcg cggctgctcc
     8821 cgcgtcggcc aataccgagg tggaaacggc cgagttgctc atcaagttgt tgcaagtcgg
     8881 tcgtggggtg atttccgaat accaagccac gatcaatgac gccagcaagg gcgacaaagg
     8941 gtttagcggc gactttgtgt ccaatcaggt gatcgaacga tttcgcaaag ccaccaagat
     9001 cgatctgagc cggcccgcca cggttccgca agcgccgctt tttctcgcca tggtggaatc
     9061 cgaaaaagaa gtcatcgacg aggcgcagcc cgtcatcaat aagcagggcg tcggcttcaa
     9121 agggattatc cccgccgtct tcgcccgcaa gtccggcgaa cggttctatc ggaagaccgg
     9181 aattcgtgtg aagttgacgg gcaccgacta tcggtttccg ggcaatcggc cggacgagtt
     9241 tgagtcggaa gtgttgagaa tttttgccga cacgcgccat ccgaaaggcc agccgtacag
     9301 caagaccacg atgttggacg ggaagccggt actgcgcatg atggatccgg aatatgccgc
     9361 gagcacgtgc ctgagttgcc atggcgaccc gaaaggggag cgcgacatca ccggcgccaa
     9421 aaaagaggga tggcatgagg gcgatctggc cggtgcgatc agcatcgtga ttcccatgcg
     9481 gtagccttcg acgaatcaac cgactgtatc gtgcacacat ttagcaggtt tgaagggggg
     9541 acgtactcat gagcaagatc gttgtcgtcg atgattcgta cgccgaactg cagttgatcg
     9601 aaggctattt gaagtccgcc aatcacacag tcgtgtcgta tccgaacacc gacaaactcg
     9661 aggacaaact ggcgttggat aagccggact tgatcgtgct ggatgtcgtg atgcccgggc
     9721 gtaacgggtt ccaggcctgc cgcgacctga aaagcgatga tcgtttcaag ggcatcccga
     9781 tcgtcctctg cacgtccaag gggcaggaaa gcgacaaatt ctggggccaa cagcagggtg
     9841 ccaacgggta cgtggtgaag ccgtttaaag ccgaagatct ggtcgccgca gtcaaacggg
     9901 cgttgaacta acccatgagc gacgtgccaa cgatccaggc tcactcctcg gactcaactc
     9961 cggctcacgg accctccagc ccatcgaggt cttctgccgc gtcgatcgaa ggcacgctgc
    10021 gtatgtgtgt cctcacgctg ggaggcgagt tgttcgccat cgatctccgc cacgtcagtg
    10081 aagtgtttga ggtggaagcg gtcaccgtcg tgcccagcat gccgggcctg ttgaccggag
    10141 tgaccaatct ccggggaacg gtcattaccc tcgtcgattt gcgaggaagc ctgggcctgc
    10201 ccgtgaccgg gaccgtgctg cctttcgcgg tcgtgattcg ccatggcagc cgacaggtgg
    10261 gagtgctcgt cgatcacgtt cctgaaattc acaccgtcag tcgcgagcac ctgttgccgg
    10321 ccatgcaagc gggaccggcg ggggcgcgtc cttgtgtctc ggcagtgcta cgcctggacg
    10381 aacgtctggg gggagtgctg gaggtgccgc aggtctttgc acaggttgac ggagggagtg
    10441 cgatattgtc gatcgcgtaa agcgcaggcg cgcgggtcat tgcgaacctg cgtcatcgaa
    10501 gattgtgcga ccggccaccg gatgttgagg aaagcagaac aaggaggagc gcaccatgat
    10561 cgggcaaggc gtcaagaatc aatttcagga catgaaaaca aagacgaagc tgctggtcag
    10621 cttcggtctc gtgagtgtca tcatcatgat catggccagc gtcggtgtgc tcaccctgcg
    10681 ccaactcagc acccagtcgc aaacggtgta tgcggactat acggtgccgt tggctgagtt
    10741 cgcccagatg ggaaccgcgt tgaccaaaca tcaccagatt ctgctggata tcgcgtccgc
    10801 caccaaacaa ggcgacttcg cccaggaagt gaccaagttg ccgcccctga aggcccagat
    10861 cgacaaggtg gtgaacaact ataagagcac gacgctgcgc gtatcgcgat cgggccggga
    10921 cgaggcgaag gatctgaccc tctttgagcc ggcattgaaa aaatactttc aggatgccga
    10981 cggcgcactg agcgccatgg cggatagctt cgatcgtaac gtcctgtcat cgacgcaagc
    11041 ggagcaaatg cgggccctcg gtatcctggc cttaacggtc aatttgacgc cctcattcga
    11101 aaatgccgtc aaacgccaca atgagcaggt tacggccatc gaggccgtcg ccaaggacct
    11161 caacgatgat gcgcaaagcc tggccaacaa cggtaccctg attctggtgg ccggcggtct
    11221 ggtcgccgtg gccctcggat tgttcgtcgg atatctgctc gccacattcc tctcccggag
    11281 catcagccac atcgccaatg tcgcgacgca ggccgccggc ggcaatctgc aggcccgcgc
    11341 gaagattcag tcgcacgatg agttgggcca gatggccacc gcgttcaact ccatgctcga
    11401 tcgtattacc gccctggtgt cgacggagga agagcgcgac ctgatgcaga aacggctcat
    11461 gcaattcctc gtgctggtct ccgaagtcgg taaaggagac ttgacccggc gaggcgaagt
    11521 gacggccgac atgttcggca accttgcgga cgggttcaac ctcatgatcg cgcggttcgg
    11581 acaattgttg aagcaggtgc gcgaggcggc cgatcgggtg aacaagtcag ccggtaccct
    11641 gcgggattca gccggtcaaa tgtccggaac agcccgggtg caggcggaag aatccgtgcg
    11701 tacgctgggc gcggtcgaac agttggccgc cggtatgcgt caagtggcca ctacggccgg
    11761 cgcatcctcc gactccgcca agcaggtgct gtcggccacg gaacgcggca acgtcgcggt
    11821 gcaggaaacc gtgcgcgaca tgcagagcat ccggtcggcg gtgcaacgta tgtccaagca
    11881 ggtgaagggc ctcggtgacc gttcactcga aatttcacag atcgtgtcga cgatccgcga
    11941 tatcgccaat caaacgaacc tgctcgcgct caacgccgcc attgaggcgg ccggcgcggg
    12001 tgaggccggc gcacgattcg ccgtcgtcgc cgaccaggtc agaaagctgg cggaaagctc
    12061 cacgcaagcc acacgtgaaa tcgccgacct cgtgaaagtg attcaaacgg aaacgcagga
    12121 tgccgtggtg gccatggagc atgaaacgca ggcggtggaa gccggatccg cttcggctct
    12181 ccggaccggc gacgtgttcg ctgaaatttc agacattgcg aaacggtctt ccgagttggc
    12241 ccagaacatc gcgagcgcgg cgtccgaaca gaccgcctcg acggaaaaag tcggtcgagc
    12301 cattaaggag ttcacgggcg gcgccgtggc gacgcagaag cagacggact cgacgcgact
    12361 cacgatcgaa gacatggcaa aactggccga aggcctcaac tcttcggtcg ctcagttcaa
    12421 gctcgcgtaa gagaattctt gaagagcggt gaccgacctc cgctcggatg ctgagtgggt
    12481 gaatgagcgc tgatttcgat cgcgaccagt tgctggacat tttcgtggcc gaggccggcg
    12541 acgatatggg gcgcttctgg aaagcgttgc atcccgatgg gaaggcgcat ccggagccgg
    12601 gcgacgtcgc cgagtttcac acggtcggcc acaagctcaa aggcgcggcg ctcctctacg
    12661 ggttcccggc gctcgggcaa ctcggggccc tactcgaaga tacgctcgaa cgagtgcagg
    12721 agattccggc ggcgcgctgg cccgaagcca tgcaactgat gcgcgagatt gccgcctctt
    12781 ttcgagggca ggtcgagcag atcgggcgtg ggggagggga agatgcctcc gtggttgagg
    12841 gatttttttt tatgatatgg gtatttatga gttctttttc gttgttttcg tcggcagcgt
    12901 ctgtgcagcg ttccgatcag ccggcggatg agtacctcat tcctgtcctg gacgaagagg
    12961 tgctgtccta cttctcgccg gaggcagaag aatacctcaa cacgatccag accctgctcc
    13021 aacggttgga ctccgacttg gcaaatgccg acacgattca tcaattgtat cgggtggcgc
    13081 acacgctgaa agggtcggcc tataccgttg gatttgaggt cgtgggagat ctctctcatc
    13141 cgattgaatc ctgcatgatg gccgttcgcg agggcgctgt cacgatcgcg ccgcattgga
    13201 tcgccgttct gcggcaggcg gtggatgtga tccgttccct gatggcgcgg gacgctcaat
    13261 cactgacgcg cttgcgtcag gaggtgcctc gcatcaggac gaagctcaga gaattggagc
    13321 agggtgtgag ccagggtgag tctgcagcgg ctgccgctga gacgaggcgg cctgaagaga
    13381 agtcggtgca ggaggccgtg cctgccgcca catccggtcc cgaatccgct gtgcactcca
    13441 tgagcgaggc ggactcccag gagctctccg aggcctatct gatcccggcc ctcgattctg
    13501 aggtgatgtc ctatttcgcg ccggaagcgc aggaatattt ggaaagcctc gaagccgact
    13561 tgctgcgagt cgataaggac gcgggcaatc ccgacacgat ccaccaactg ttccgcacag
    13621 cccatacgct gaaaggctcg gcctataccg taggctttca ggcgatcggt gatctgactc
    13681 atcacattga agacttcatg ggcgccgttc gcgaagggca gctggtgctc cttcccggtc
    13741 acacggatgt cctgttgcgc tcaatcgatg tcattcgcgc cttgatgcga cgggatgtat
    13801 ccatggtggg gcgaacgaga caacggtttg cgaactcctt gcaggagttg aagcaactgg
    13861 gacaggctca gccggcaccg gaccaagatc accccatcgt ctcatcgatt gccgaaggcg
    13921 cctcggtgga agaaattcgc gaagccgacg gacaggattc ggccaaaggc gtcgatggga
    13981 agaccggcga agagcgagag gtgatccgag tcagccgtga tcgcttggaa cgcctcctca
    14041 atctggtcgg cgaattggtt attgatcgcg gccggctgga gcagcggctg cgcacgttgg
    14101 atcaattggc cacacaggtc ttggccaaca agaaccgact gaccgatgcc gtacgcacct
    14161 tcgaagataa acataccttt tcctttcagc cctctcccac atcctcgagc gaagccgcgc
    14221 cgcaggcgta tcctggcgtc agtgatttcg gcagcctgga attcgacaag tacgacgact
    14281 tcaacatcct ggcccggcga atcagcgagg tcaccgcgga catcaccgaa tcgatgtcgc
    14341 agctgagcgg atcgatccat cgcgcccagg acgacatggg gtcgctgcaa cagttgacct
    14401 tgggcatgcg cgatgaaatt gcccgcgcgc gcatggtgcc gatcgggacg ccgttcacgc
    14461 gcttccgccg cgcggcccgt gagatggccc gggccaccgg aaaagaagtc aatttggtca
    14521 cgtccggcga acataccgaa atcgacaccg gtgtcgtgga gcggcttgtc gatccgcttg
    14581 tccatctggt cagaaatgcg gtgtaccacg ggatcgagcc tgcgggcatc cgtcgcagcc
    14641 aagggaagcc ggcggccggt acgatctatc tccacgccgc gcatcgaggg aactcggtgc
    14701 ttatcgaagt cgaagacgac ggagcggggc tcgacatcgt caagatcaag gccaaagcgg
    14761 tcaagctggg tctggtacga gcggatgtcg cggactcact gccggagagc gaaatcatca
    14821 agttcatctt cctgccggga ttttccactg ccgaggcggt cggggatcag gccggacgcg
    14881 gcgtcggcat ggatgtggtc aagcgggtca tcgagacgat gaacggccac atcgaggtgg
    14941 aatccgtcta cgggcagggc accaagttca cgatgcattt gccactcacc ctcttgatcg
    15001 caactgcgct gctcgttcgg gtcgggaaag agcgctacgc gattccgctt ccgagcgtgc
    15061 gtgaggtcac aatgtcgacg gcgtcgacga tccagcagat gggggatcga tcggtgctgc
    15121 agatcggcga cgaagccatt gaggtctatc cgctcggcag cctcattcgc cgggatgccg
    15181 gcaccattga gggagcgact cccgtggtcg tcgttcgaac ctcgaccggc gcgcttggtt
    15241 gcgcggtgga tgaattgctg ggccggcagg aaatcgtcat taaatcattg ggtgggctga
    15301 agccgtatga acggtccgtg ttcggtggcg cgactatcga tcccgagggg cgtgtggtgc
    15361 tggttctgga cgtcagccgg ttgacgactc gcgaatacca tgaggccatg tcggtcattc
    15421 aaaaccccgc ctcctcgccg atgcttgagg gaccggcgca gcccgcttcg ccgcagcttc
    15481 cggatacgca gcttccgctc ttgttgatcg atgactcgct cagcatcaga aagtttgtcg
    15541 gacgcatgct ggagtcggcc gggtatatcg tcgaaacggc gaccgacgga gaagaaggct
    15601 gtcgcaaggc attggtgcag cagtaccagc tcatcatcac cgatctcgaa atgccgaagt
    15661 tgaacggctt tgaagtcatt caggcgcttc gcgccaggcc gcagactcag accacaccga
    15721 ttctcgtcat gaccacgcgg gcgggtgaaa agcatcgcca gatggccgtg agtgtggggg
    15781 cctcggggta cattgccaag ccggtggaag aacgcgccct gatccaggaa gtccgcaagt
    15841 ggacaggccg cgaaagcgtc gtgaaaaaat aggaacgttt tgtcgattcg taccgttact
    15901 ggcagaatga aggacaggca tggggctccg gggacataag gcagcagtgg tgtcgagcgg
    15961 gttggcggtc cggtttctcg tggcgctcat gggccccacg catgtggcct ttccctccca
    16021 ctgggttcgc ggcattgtga ctccggctga gggaggacag gacgggcatg tcacctgggc
    16081 caatgcttcc tacgaacgca ccgatctcgc ccgtcgtttg acgatccagg ccaaaggcgt
    16141 cacggctgag acccggatcg tgctctatgc caatgagcaa cgatcgcggt cctttgcggt
    16201 ggacaaggtc gtgggcctca tcgatgtcga acggacgctg atccagccgc tccctgcgca
    16261 gtttcgggga ggggagcggg agcgattact cggattgttt gtcgagtcgt cgtatatcgc
    16321 gctgatcgcc aatccgtttt gggtgctcga gctcccctcc cggaccaatg tcttggatgt
    16381 gtttgccctt cgggtgtcgg agcgccggcc gggcgaattc gattccagac tccgcctgcc
    16441 ttcggcggcc ttggaagagg cgtcggcgat gtcggccgga tcggccaagt aggtcacaag
    16501 gatcgaccat gctacgtact cagctaggca gacaaccgca caagggtagc aaaggcgcga
    16561 ccgaggcgcg gatgcagcac atggtggtgt tttcggtggg cgggcaacgg ttggccgcca
    16621 gaaccgagga gatcggcggg gtgatgccgt ggacgggctc gacggcggtt cccagcgata
    16681 cgccgtttgt gcgcgctctc gtccgacagg agaagggatg tcttccggta ttcgacctgg
    16741 cggcgaagtt caagcgctcc ctgcaggatg gtgaaccgct gtgtctggtt gtcaaacatg
    16801 tggatggacc gctggcaatc tgtatcgatt cccaggttcc ttccttgtac atggtggcac
    16861 gctcggcagt gcagtatcgc gccggcaacg atcccgacat cgcgggaacg tgcatcgccg
    16921 gcgaagaaga acttcctatc atcaacctga caaccttggg ggtctcatcg aaccgccccg
    16981 cgtgatgagc cttgccttgt gtagaggaac gacatgccaa agatcttgat tgccgacgac
    17041 agtatcgcag tccgtaaggt ggccgagcgt ctcctgaccg aggccggcat gggggtgaca
    17101 cttgcggcga acggatctga ggctctggcg ctgttaagta aggatcgccc cgacctcatc
    17161 gtgtccgatg tcatcatgcc ggacaagagc ggctatgagg tctgtgcgta cattcgggga
    17221 caggcaaacc tcgcggatct tcccgtgctg ctgatcagcg gtatcgtgaa cgatgaagtg
    17281 tcccgacaag cggagtcctg taaggccgac ggggtcttga agaagccgtt tcagggctcc
    17341 tcactcaagg accgtgtgct ggacctgttg acgaagcgcc cacaaaagcc tgctccggtt
    17401 ccggaactgc cgcagcagca cgtggccgtg gagccatcta tcgtggcaga ggtcgcggtc
    17461 gcacccgcac ccgccatcca tgtgccggaa gaactcgaag aggcccgcgt agacactcag
    17521 gagcatcggc cgtcgctcga gccgatcacg agcagtcttg acgatcctca cgccataccg
    17581 gcgacgatga cccgcgacac gatggtggat gcggcccctg cattccatgc cgctgagccg
    17641 gctacgccgg cgcatgatca tgagcatcat catgcaatcc tggcggaacg tgacacgcgg
    17701 atcggcgaac tcgaagcgca gttggacgag gagcgtcggc ggagtctgga gcagacccag
    17761 cagatcgggc acatccagcg tgcgttggat gagcagcacg agcaggttgc agccctgtcg
    17821 tcacatgcac acacattgga ccagagtcta gccgacgagc gggcgcagcg aaccgctctc
    17881 gcagaacagt tggatggagt ctcgagacag atgcaccgga tcggcgaact ggagacggct
    17941 ctggccgatg agcagcatcg tgcccagcaa ttgtaccaac aggtttcgga aggggcccgt
    18001 catgccgcgc gcatcgtgga attggaggcc catctggagg cagagcggga agcggcgaat
    18061 caattggtgc agcagattac cggtcttgag caggtcgaaa cgcgcgtgca cgagctcgaa
    18121 acgaccttgg ccgctgaacg tgagcaagcg gaaatgcagc gacgggagcg tgtcgagctg
    18181 gaacaggttg cagacaaggt gccggccctg gaagaggcct gtgcgaaagc ccatgctcag
    18241 atcgccgagc tggacgcggc gctctcggct gaacgggagg ctgcggcggc gctgttggtt
    18301 caggtcaaac agttggaagc gacggcgcaa cgtgccagtg agctggatct cgccctggtc
    18361 agcgagcagg agcgttcgat gcagctcgag aaacgggcga tggaagccga gcacatggcg
    18421 gagcagtcga ctcgccggtt cgaagatatg gcgaggaagc tgggggagat cgcggggctg
    18481 gcctcccaac tcggtaatgg aaaacgctag gaggccgtca ctcgtccgta tgttcaggcg
    18541 agtgcatggg tcagaggcag ggcgccactg tgtcgaccga accagaacag accgaagacg
    18601 attttcaaaa agaactgatt gaactgttcg gtcaagaggc gcaagagtgg ctcatccaaa
    18661 tccattccgc cctgaccgaa ctggaaagcc agccggatac cgatcggcat gcgcaactgg
    18721 tggatgccgt ggtgaggggc atcaccagtc tcggtggttc tgccgccacg gtgaaccttc
    18781 ccgatgtcga acgcgctacc ttcgcactgc ttcccttcat cgacaccctg aaagaccgga
    18841 cgacggcgac caagcaggat ttcggcacgg tccgtgaaca gttctgtctc gtcatcgcgt
    18901 ccgtcaccag ggccactggc attacgctgg atcttggtcc tgagcagggc gcgcctccac
    18961 aaccggaacc ggccatcgat ttcctcacgc tcctgaacgc gcttcgcatg cttcaggacg
    19021 agcaggttgc caaggaagtc ccttcccgca gtttgattcc acaagtcatg caccgattgg
    19081 agcaggaagc gagacaaggg gagagccaga tacagatgac cgcgttccag cagctgctgg
    19141 gtgagctcca aagcacggac gcccgatgcc tggactcact acgacaagca ttgcccggca
    19201 ttgcgcagaa cctgaacagg ctccgggtcg agggattgac ggttttggaa ccaggcaacg
    19261 tgctcggcac ctgcatgcaa cagatcgaac aactccaagg cattgccaag caggtcaatg
    19321 ccaccccgct ggtgaccttt ctcaccggcc ttcagaactt ccttgccttg attgtccagc
    19381 gtcgtatcgt gatcgcgtcc caacgtgtcc aggccgtcga agcacgcatt ctcgccgtga
    19441 tgaccacgat cgaggactgg atcaccgccg gacaacagga acgtgatctg atgagccggc
    19501 tgctgcctgc cgcctgagga cgctcctcac agtgcgcgac cggcctcgga caggacttcc
    19561 cggacaggct tcgttgagtt gaaggcgcac ctccaccatg gggatcattc cggacggctt
    19621 cgctgattcg cctgactcgg agcgttggac catgcagagg tgaaaacaaa ggcgtgccgc
    19681 gtgacgaagc gcgtcgaggc tggaggctaa gaggaaatgg agcagaatca ctaccggcag
    19741 gctgaggatg cgctgatgcg ggtcgctgcc gccgtgcaac ggcaggaacc ggtgaacctg
    19801 acggagttga cccatcttgc cggcagcatc gtcgagtcga ttcaggaaac tgatcagtta
    19861 gtcgttgaag cgctctccag tcctgtcggc cctccgctgg ttaccaatct agtcaatgtg
    19921 agcattctcg cgacgaaggt cgggatcggc ctcggctatt acggcgcgga gttgcggcgt
    19981 ttggcgttgg ccgggctcct gcacgacatc ggcatttttg ccgtgccgca gcatctactg
    20041 acgaagaccg gccgtctgag ctccgatgag cgggctctgg tcgagcaaca tccgcgtttg
    20101 gggtatgggg tgatcgaacg tctcggtgcg gactatgcct ggctggccga agtggtattg
    20161 caggcccatg agcgcgggaa ggggcagggg tacccaaacc gtttaaaggg gcgtgaaatt
    20221 aatgagctgg cgcagattat cggactcgtc gatatcttcg atgcgttggt cagtccgcgc
    20281 ccctaccgcc ggcgcctcct cccgcacgag gcgatccgtg agttattgaa cacggagcgt
    20341 actgcgtttc cccgtgaaat catgaaagga ttggtcgagc aactctcggt ctacccgctg
    20401 ggtaccaaag tgcgcctgag cagcggcgaa gaaggggtgg tggtgcgaat cacgcctcgc
    20461 tacccatccc gtccggtgct tcgtgtgacc ggcgcacagg agacagcggt gaccgatccg
    20521 ccgcggtttc tcgatctcag tcagttgccc caggtgtcgg tcgcagaaac tgtcgattca
    20581 ccggcattgg agcgcgtcac gtttgcaacc gcaccgatgg ctgccgagcc cgccacgccg
    20641 acgagcgcat cggatcagtt tgccgcctta ctcgaaagtc tggatgcgat cgccggcgtg
    20701 attcaggccg cggtggagca acccaaggca gctgcgcagc ctgcgcaggt ccagtcgacg
    20761 atcgaggcgc ctgaccaggg cccgcatgtc cggcgggaaa ttctcggcct gtttgtattg
    20821 gaggcccgtg aatggctcaa tcagatccag acggcgcttg agcggctcga cacgacaagt
    20881 gaacaggggc gccgcgccaa gttgacgacg attttatggc agagcgtcag caacctcgcg
    20941 caatccgccg cgaccgtcgg gttgacggcc gtggaagaca tggcgacccg actgcttccg
    21001 ctccttcagg ccgccgcaaa acatgagcgc accgtggcgg ctcatcatgt ggcgtcgctt
    21061 cgtgaggggc tgctcgaaat cagcaagacg gtgcaggcgc ttcaggcgct cgagcggcca
    21121 ccccaggatc atctcgaagc tgccgtcgct gtgcagcagc ctgtgactga aatgcctgag
    21181 ccgatggtcg ctgaagcgcc ggcccctgtc gaggctgacg ttccgtgcga cgcgccttcc
    21241 acttcgttgc cggccgcatc tattcttgac gccttgcggc agttgcatca ggcgcgaggt
    21301 cgatcgctgc aaccggtgcg cgatgtgttg gaaaccgtga ttcagcgtgc cgagcaggag
    21361 atggcgcgag ggacaggcgt ggtggatgcg cgtgccatcg ggcgactgtt gcaggagctg
    21421 gatgagttgg acgagcgatt tctcgcgcac atgcaggcgc atatcccggc ggtgatcgcg
    21481 acgctcaggc atgtcgctct caccagtgag gatcgtgtct tcccacccca ggcgttggaa
    21541 cccatttttg tcgagatcga ggcgctttcc gatgcggccg accgggtggc cgcggcgaat
    21601 atcagcctgt tcctgcacgg cctgcgtaca ttcctccgcg tcacggccca gcacaagccg
    21661 atggtgatcc gcgagcgatt ggcagccgtg gaagaacgat tggccacact cattccgctg
    21721 gcccagcaat gggtcgatgt cggccgcgtg gaacgggcgg ccatttttga catcctccct
    21781 atggcgtagc ggcttgacga agcgctccca attgataccc gcgttgcctg cctctccgcg
    21841 tcgcctgccc tcaaccgctc aaagatgaac actgttccat cctgctgaga aatctcacgt
    21901 catgccgcct aattattgag cacattgcgg gcattcctac gcagccactg tcggcggagg
    21961 tctgtccaca tggttgcgca cagtccgtcc acagttcttg tggacaggga agaatgatgc
    22021 cactcagttg agatcgccgg ttccccacgt caatcctgaa cgatcgatcc accgcctgct
    22081 gaacacgtgt tgctgtgcga gcttggaggg aatcaactcc gtcggcctgc gccccttgtg
    22141 ctcacgcctc ttgctcgtcg agtgaagagg ggccctgcac ggaaaaggta tgcttgcttc
    22201 gctgctcgct gaaatttgtc ccggtccgac acattttcgc ttgcggttga ggttgcgatt
    22261 gtacgaccat cacgcccatg gcaaaactga tctctctcaa gcagcaggaa gggcacgacg
    22321 cgcgggcgac ggcctacatc aaagcgtaca tgttatttcc ggccggcatc cttggcctga
    22381 tttccatgat cggcggggtc ggcgggctcg ggtatcagct catcgccacc gacagctaca
    22441 cgtggtggac ctttctccaa agttccgggt tgctgctgct gggcggggtg ctcgggtggg
    22501 tgcagacgac gtaccaccgg tggatcttga ccaatcgccc ggaggtgttt gcggcgcgca
    22561 tgcggcagcc gacggtcagt aagagcggca aacccaaacg agagagtgcg gccagtcagg
    22621 aacaggccag cgggtcaccc tgggctccgg gcgcctatat ggtcggcctg gcggtattgc
    22681 tcgcgggctc aatggtcagc atgatctatg gcgcggtcca tccgatcgcg gcctgtttcc
    22741 tgccctgggc gggatttttc tgggccaagc tgttcttttg gaagtcgatg ctgaagaact
    22801 aacgatgcgg ctgacgcggg ctatttgcga cggggcttgc gggacttggc gggaggttgt
    22861 cggatctgtt ccagtttcgc aaggcgctct tccaactcgc ggacgcgctg gcgtagttcc
    22921 ggcaactgcg gaatcaccgc ctgggccttc agaaacgtcg catggggcat caccggcgcg
    22981 ccggagacga tttgattggg ctccagactt cttgtgacgc cggacttagc cgcaatcatc
    23041 acctgatcgc cgatctgcaa atggtcggcg aggccggcct ggccacccac catcacatac
    23101 tttcccagcg tcgtactccc cgcgataccg acttgcgcga cgagaatatt gtgctcaccc
    23161 accaccacgt tgtgggcgat ctgcacgagg ttgtcgattt tcgtcccgcg cttgatgacg
    23221 gtgttgccga acgtcgcgcg atccacgctc acattggagc ccagttccac gtcatcctcg
    23281 atgagcacgc cgcccaattg cggaatcttc tggtgacgtc cctgatactg gacgtagccg
    23341 aatccgtcac tgccgatcac cgtgccgctg tgcacgatca cgcgagctcc gagacggcag
    23401 ccttcacgaa ccaccacgtt ggggtagagg agggcatcat caccgatggc gctatcgtca
    23461 ccgataaaga cccccggata gagggtcacc cgcgcgccga tggagacgcg gtccccgagg
    23521 gtcacgccgg gccagatcga cacatccgcg ccgatggtga cgtcctcgcc gcgggtgatg
    23581 ccctgggcga tgccgcgcat gcgggtcggt cgcgcaaaaa actgctgggc ggcccgtgca
    23641 aaggcatacg caggattgtc caccacgagt tgcggtgacg ggcaatccgg caggcgacgt
    23701 cccacgagca gggccccgat agtggcggtc tgcggagatt tcaaattgcg ttcggaggtc
    23761 aggaaggcga ggtcgcccgg tccggcctct tccaggctcg ccactccggt gacggtcgca
    23821 tccggtgaac cgaccagctc tcctccgaca tagctgtgga gttccaggag tgtcagcggc
    23881 gtgtgtctcg gtgcggtcgt catacggcta ggccttcttg ctcttggtag aacgcttggc
    23941 ggcaggtggc ttcgcggcgg cattcgccgg agttttcgcg gccgccttcg cgggagcctt
    24001 cggaggggcg acgcggccct gcacataggc gatcacctgg ccgacggtgg tcaggccgac
    24061 gaggtcctgg tcggggatct ggagattaaa gacttcttcg atgcggtaca gcatttcgat
    24121 caccgccatc gaatccaaac ccaggtcgtc ccgcaggtga tgggtcgtct gaatcgacgc
    24181 cgcatcccgc ttgagatagt ccgccagcgc ctgaatgatc ttgtcggaaa cgtctgaagt
    24241 cttagccata accggttcct tcgtttcttg ccggcacatc gcaggtgcac gaatgtcgtc
    24301 gcccgacgcg ggtcaggtcg acagagattt taagacgacc gcggcattgt tactgccgaa
    24361 gccgaacgcg ttgatcagcg cgaccttggt tttgcgggtt tgcggttgcg cagacagccc
    24421 cgcgagggcg caggccggat ccggatcgtc aaggttgagc gtcgggtgga tctgcccgct
    24481 ggtaagggtc agggccgaga cgatcccggc cagcgatccc gcggcaccca gcgtatgccc
    24541 cacgagtgac ttcgtggcgc tcaccgccag cttatccgcc cgggatttga acaagagacg
    24601 aatggccttc acctcgaccg catcacccac cgtcgtggaa gtcgcatggg cattgatgta
    24661 atcgacttgc gcaggcgtga cgcccgcatc cttcaaggcc aggcgcatcg tgatggccac
    24721 ctcttcgccg tcctcgcgtg gaatcaccat gtgatgagct tcgctggtgg cggcgtatcc
    24781 ggccacttcc gcgtacatcc gggccttgcg tttcttggcg tgggcgagcg attcgacaat
    24841 cagcgcgccg gcgccttcac ccatgacaaa gccatcccgt ccgcgatcga agggtcgtga
    24901 ggctcgttcc ggtgcatcgt tatacttggt cgaaagtgcg cgcagcgagc aaaatccggc
    24961 aaagaccagg ggcgtaatac tggcatcggc gccgacgacg atcaccgcat ccgccgtgcc
    25021 ggcgcgaatg gcgtgcatgg cctgaccaag ggcatgggcg ctcgatgagc aagccgtgga
    25081 aattgtcagg ttaggtcctt tggccccata ggccatggcc acgatgccgg aagccgaatt
    25141 cagtgtaatg accggaataa agttgggatg tacccggtgc gggcgtttct gttcatacag
    25201 ctgcgtgatt tcccgttcgc ccatcaccat gccgcccatg ccggcgccga cgatgacccc
    25261 gacacggtgc ggctcttccc gctccatgcg gaagtcggcg tccgccagcg cttccttggc
    25321 ggccacaagg gcgaactggg cgtagcggtc cacccggtcg ccttgaccgg ctgggagata
    25381 ctgttcgggg gaaaatccga gaatgcgtcc ggccacgcgc gaccggtacc cttccaacgg
    25441 gaacggatcg aaggaggtaa tggccgagat cccggaccgg ccttccaatg ccgctttcca
    25501 gaactgcggc accccgatgc cgatggggga gacgactccc agacctgtga tgacgactct
    25561 cgtcgacata gaaccctttc actgcttgcc ggtgatgcct tgtgcggcct gtctacttac
    25621 cgtacggtgg cgccacagtc aatgctgtaa gcatcaggca ctcgcgagcg atgctgcgac
    25681 tgtcgaatgg cgaacgccgc tggcggacta tttcgcgatc ctctcagtaa cggaccttca
    25741 acagcagata aaagccaaac gtcagaaata ccagattggc catccagccg gccagcatcg
    25801 gcgcaagtgc cccgccgcgt cccagcgcaa ttgccacgga atgggtggac caataaaaga
    25861 atcctacggc catggcttgt ccgatgccca tggccatgcc gctgccgcgc acgccggtgc
    25921 gccggaggct caaggcaatg ccgacgacca ccataataaa ggtcacgcag gggaaggcga
    25981 tccggccata ataatcagtc agcaggcgcg ggaggatcgt gccgcccaga tgcagccgat
    26041 cgacataggc acgaatctcc ggcagcgtca tcgtttccga atccaactcc agccaggtcg
    26101 tgaagtcgtc gggaatttgc gcgagcggca ggggttccgt ctgaaaactg ttcaccgaga
    26161 ccgtcccatc ctgccggaac gagcgccgga tgccgtcttc caggacccag ccctgtggcg
    26221 catacaccgc gcgctgtgcc tccgcaatct gctccagctc gaacgtgggg ctcaagcgat
    26281 agaggcggat ttttcgcaac acggctccgc cggggtcgat ggtgtcgatg ttcatcaatg
    26341 tttggccgcc caggctgacc cagggctgcg ctgctttcac cgagaccggc aactctcgtt
    26401 tttcgatccg ggtcgttttg atctgctcgg ccttggcgag ggcgagggga atgacggtcg
    26461 aactgaagag gaacaggacc atcgccagca cggtaccgag aaacaggaac ggggaggtaa
    26521 tccagtagag actgataccg caactgcgca tcgcggtgat ttcatggctg cgcgacaaca
    26581 ggccgatcgt gagcagggtc gccatcagga ccgcgagcgg cgcgatctgg tacgaaatag
    26641 cgggcatctt gaggacgaag taggccagca catcgagggc atgggcgtcg taccgcagga
    26701 agcggcgcac cttctcgaag aagtcgatca cgaggtaaat cgtcatgagc ccggcgaagc
    26761 acatgccgaa gatcttcacg tactcgcgca gcatatagcg aaacagaatg ttcataggac
    26821 tattggcggc tcacccgata gaaccacaga atggtcacca gggtgaagat cacgttgggt
    26881 aaccaggcgc cggcgaacgg ggaaatccac agggtggtga ccaggaactc gcaggccaca
    26941 ttgaggacat agtaggcgat gaccaccagc acgcccaccg caaacccgcc gatgctgccc
    27001 gaacgtttgg acacgatgcc gaccggcact cccaggatac agaacaccag cgaggccgca
    27061 gggaaggcca ggtccttata atattccatc aagcgccgca acgcgttggt atccgtccag
    27121 ccggtgctct ccagcttggc gcgaatgacg tcgatcggcg tgcgttcctc cgcgccgtac
    27181 aggctcgcgc tcaagctcag cttcaagtcg taggacgtaa acgaaatctt ctgatactcc
    27241 tccggattct gggggcgact atggatgacg ccgttcatca gccgtagcgc cacctggctg
    27301 ctggccggat cagtcatgac ctgatactgc tgcgcgacga tgatacgtgg atcggccggg
    27361 ttccgttcat cggccacgaa aattccccgg ttgtcctgtc cctcctgggc gtccggtacg
    27421 tagatcgtca tcttgggaat ggcttcatta aagacgccgc gatcgagttc caacacgagt
    27481 tcgtcgcgca acaggttcag cgccaccttc ttgagattga cgttgctcca gggttgcccg
    27541 tactgcgcca tcaccagcgt caggccgcag accagcccgg cgaaaataaa caccgactga
    27601 gacagccgca ggaggctgaa gccggcggcg cgcatcgcca cgagctcctt gtcgagggac
    27661 aagcggccga aggcggtaat ggtggcaatg atgcccgcaa tgggcagggt gaggaccaga
    27721 aacgacggca gcaaatgggc gaacactttc aggaccgaga gaaagccgat gcccttcgtc
    27781 accagcagtt ccaccagccg caacagctct ttcgtgagca tgacgaaaca gagcgcagcc
    27841 aggctgatgc cgaacggaga gaagagttcg cggaaaatat agcgatcgag gatggtgtgc
    27901 agcacgaggg cggtcgaaat cctacatcgg agacttgagt tgccgcacta tagaagagtc
    27961 tacggagctt gtaaacagat ctgagagcca ttctcgaaac ttcccgcttg acaagatttt
    28021 caccccatgg tacatcgctg cgcacttttc agtaggtcac gatggttcgg gttgaatatt
    28081 gtctcaacgc ctcgcaagag ctttcacgct cgtcggtttt atttctcctt ttatcgtcat
    28141 cattaccatg ccatgcgact cagccggcga gctgatgcgc tcattatgtc tgtgcgttga
    28201 gaaagggagg accgtatgaa gaccaagggg acagtgaaat ggttcaacga tcgtaagggg
    28261 ttcgggttta tccggctcga tggcggagac gatgtcttcg tgcattattc cgcgctgcag
    28321 ggcgagggat tcaagacgtt gaaagagggg gagaacgtcg agttcgacat cgtgcaggga
    28381 gccaaggggc cgcaagcggc gaacgtgttg aaggatctcg caacggcctc ctgattgccg
    28441 gggcatcgta atccgcggcc cctccgtgtt ccggaggggc cgccggtttg ccatcgttga
    28501 tttcgtccgc tctggacgcc gcgttcgcgg tgttgctctt ttatccattt gtttgacaaa
    28561 gaaaaggcag actgttagcg ttgactctca acgtcgggtg aggagtcttc ttggctgtcg
    28621 atcgccgaac agttctccaa aacgcgcagc tctttgcctc caaggggcag tacgaagctg
    28681 ccattgccga atggcgaaaa ctcaccaccg ataccccggc cgacgggacg attttcaact
    28741 ccatcggcga cctccagctg aaacgcaatg ccacgcccga cgccgtcgcc gcctttcttc
    28801 aggccgccgg cgccttccgc tccgaagggt ctgtcctcaa ggccattgcc gcctataaaa
    28861 aaattctcaa ggtcgatccc accaagatcg acatctatcg acacctcggc gacttgaatg
    28921 cggagcgagg gttgttgagc agcgccgttc aggattattt gaccctgggc aagcactatt
    28981 ttaaggacgg ccgcaacaaa gaagccctgg agatctaccg caaaatcatt tcacaggatc
    29041 cgtcgaacct ggatgcgcag ctgcgcgtgg cggaactctg tgagcaggaa caattagtcg
    29101 acgaagcgat tcgcgtgtat ctccaactcg ggcgggagcg ttcggccgcc caacagtatg
    29161 aacaggccaa tgccatgtat gcggcggtct tgcgcctgaa tccggagaac gccgaagcca
    29221 aagaaatcgt caccatgatc gaggcgggcg gaggcgcgcc cttgaacctg gcacgcgtcg
    29281 ccaaaccgtc tgcggccgcg gcgaagccgg gtgaaggcag cgatctcatg gctgaagcga
    29341 cgcgccggat cgaagaaggg cagtatgccg gcgccgaagc catgttgacc cagctgctga
    29401 gccgggagcc gggcaatccg gaaatctgcc aactgctggc gcggctgcat ctgttgcagg
    29461 gaaatttgca ggtcgcgttg ggtgaatatc gattcctggc cggcgccgcg ctgcgcgcgc
    29521 aagactatgt catggccgag tcgttgatta acgactatct caaggtcgaa ccggcatccg
    29581 tgccgatgtt ggaactgctg ggcgagctct atcaggaaaa aggggatgcc gccacggcag
    29641 cgcagcattt cggcagggcc gtggaaattt tgctggaaca tccggaaccg ggcatgccca
    29701 cgctgccggc tgagctgttc gagaaggtcc aggccttggc cccgggtagc gccattgccc
    29761 gcaagctcgc gcccgcgttc gacccgactg cctcatcgtc caccgaaccg gaagtcacag
    29821 agccgatggt gatgacaccg gagcctgtcg cgatccagct cgaaccggaa ccacaaccga
    29881 aaccggctcc cgctccggtc gtcgccgcgc ccgcggtaga catgccgttc cgattgcagg
    29941 acagctcggc tactccagcc gctccagcgc ccccggcggc gcctgtgctg gctcaggtcg
    30001 ccccgccgcc tccacccgtt ccggctccgg cgccgcccgt tgcccaggcc gcacccgtcg
    30061 agcctcctcc ggccccagtt cctgctgcgc aacccatcgc ccaggtggac gcggaaacgc
    30121 agtacgcgct gggcatggcc tacaaggaca tggggttgtt ggaagaagcg aaggaagagt
    30181 ttgtctcttc gatgaaggac tccgggtttt tcgtcgattc ctgcctgatg ctgggactct
    30241 gcctcaagga acagaaccgg ttcgatcagg ccattcagtt gttggagaaa ctcatcgccg
    30301 actcgcgctg ccggggaggc aatgcgcagc tcgtgcggta tgaactggcc atgctctacg
    30361 agaaaaccgg cgcccgcgat cgagcgctcg ccatgtatca gtccattccc tcgttccacg
    30421 acgtgccccg ccgggttgaa gcgctgcgca ccctcggcac aaacggggtc gcccatccgg
    30481 aacttccggc ttccagtcag accagtcccc tgccactagc gggccactaa acctcgcttt
    30541 acctagaaat cctgctgctg atgggctgat gcgagggtag aggtcctacg cctgcgtgcg
    30601 attcacctga ttcggagccg gcctcccggc gcagactgcg gcaacattct ccagacagat
    30661 catccccata cgcacacgtg tcgccagggt tgcggagccg aggtgcggga ggagcactac
    30721 ctgccggaga tcgcggaagc tggggtgaaa gaccggctcc tgttcaaaca catccagccc
    30781 cgcgccggcc agccgacgtc gcaggagcgc atcgaccaac gcgccttcat ccaccaccgg
    30841 acctcgcgag gtattgatca gaaacgccgt gggcttcatg agtgccagct gccgcggacc
    30901 gatcaagtga tgagtctccg ggctgagcgg cacatgcaga gtcacgaagt cggcctccct
    30961 cagcaggtcg ggcagggcgc gctgttccca ttgcgatgag ccctgtcccg gcgcatgcgg
    31021 ggtccgtgag ctgtaacaaa tgcgcatgtt gaacccggcg gcccgcttcg ccaccgcctg
    31081 tccgatccgg cccatgccga cgatccccag catttttccc gacacatccg tccccagcat
    31141 ctgagtcggt gcccaaccgg gccaagcgcc ggctcgcacg taggcgtcgc cttctgccac
    31201 ccgccgcgcc accgccagga tcaaggccca ggtgagatcg gcagtggagt cggtcaagac
    31261 gtccggagta ttcgtgacga caatgccctt ggccgtcgcg gcggcgaggt cgatattgtt
    31321 gtaacccacg gcgtagttcg ccaggatctt gagtctggtc ggctcggcca gcagagctgt
    31381 atccacgcga tcggtcaggg tgcagatggc cgcatcggct tcgcgaaggc ctgccttgaa
    31441 agtctcccac gagggtgcga tgtcgcgcgg ctcattgaag agttgaaact gctggcgtgc
    31501 tgccgccatg accggatcgg gcaacagtcg ggagatataa aggatgggag gtgacatggc
    31561 gcctcgttcg aatggcacgt atcttagcga atcgtgtgcg acgcaacaac tcacttgtcc
    31621 tgctctctgc cgagcgatta caatgacgca acgtaatcac aagcatgacc ctcatccttc
    31681 ccaacacctc atccgtcgtc gtcagcggcc ttcggcaact cctcggacag gcgaaggtcc
    31741 ggaacgacgt gcccacgctg accgcctacg ccgtcgatgc cagcatttac cggatgcagc
    31801 cgcaagccgt cgtgctcgcg gagcatgaag gcgacatcga taccgtcgtg cgatttgcgg
    31861 tggagcgggg catccccctc acgccgcgcg cggccggcac caatctgacc ggctctgcga
    31921 tcggctccgg catcatcctg gatatctcca agatgaatcg tatcctcgaa gtgaatgagg
    31981 aggaacgctg ggcccgcgtg cagccgggga tcgtcctggc tgaattgaac aagcagctcg
    32041 ggcgacgcaa tcttctcttc ggtcccgacc cgtccagcgg agacatgtgc aagctcgggg
    32101 gcatgctcgc caacaattcg gccgggcccc acaccctcat ctacggcgcc gttaaggata
    32161 acgtccaggc catgcgtgtc tgcctgccct ccggctcctg gctctccgct tcctcgatga
    32221 acctcaacga tccgggactg gatcagacgc tcgtcgccca tccgtcgctc aagccgatcc
    32281 tcgaccttgt ccagcggcag cacagcctga tcgacagcaa gaagccgacg gtgagcaaga
    32341 atagttgcgg gtataacctg tttggcctcg ccgacggcct aagtcgcggt gtcttcgatc
    32401 tccccaagct gtttgtcggt agcgaaggca cgctgggcgt ggtgagcgaa gcgacgcttc
    32461 gtctcgtgcc gaaaccacag ggcacgctga cggcgttgat tcactttcgc cgccttgaag
    32521 aagtggggga agcggtgccg catttgttga gcctccggcc gagcgcgctg gaagtcatgg
    32581 atgcgaacac gctgaacttg atcgggcggt cggcgcatgg cattcccgcc gatgcggccg
    32641 ccacgctgct ggctgaactc gacagcagcg aaggcgaggg cgacctgcgc gaacgggccg
    32701 atcaaatggc tgccatctgc ggacgctatc aactgtgcgg cgatctcacc atcgcctacg
    32761 acaaggagca gcgcgaccaa ctctggaaag ccagaaaggc cctgtatccc acgctctacc
    32821 ggttcgatcc gcgcaagaag ccgatcaact tcgtggacga cgtggtcgtg cccgccacac
    32881 ggatcagcga gttgatccgc tacctggaaa ccttctttga ggggcagcat gtgccggtgg
    32941 caatcttcgg ccatatcggc aacggcaacg cccacatcac gcccttgctc gacgtgaacg
    33001 accgccagga cttcgacaag atggtgcggg cctatcatga gatccacggc gcggtgctct
    33061 cccggttcga cgggtccatt tgcggcgagc acggcgacgg ccgtgtgcgg gcggaatatg
    33121 tgcgcaagat gttcggggag gagttgtatc aactcttcgt gcaggtgaag cagacgctcg
    33181 atcccgcgaa cgtgatgaac cctggcatca agatcagcga gacgccgttc accgaacaca
    33241 tcgactacca gcggctgtcg aaatcctgtg cgacctgtgc aaagtgcaac tccgtctgtc
    33301 cggtctacga cgtgttccag tcggaagaca tgagctcgcg gggctggttc gaaatcgtca
    33361 cggcgaagga ctacagctac ctcgactcga agcgggtggt ggaagcctgc ctgaattgca
    33421 aatcctgccg cacgatctgt ccggccgggg tggatgtgtc ggagctgatc ctgcagcggc
    33481 gcgccgagaa tcccaatcag ggctcgcgct ggctttttgc cctgcaggcg aagttgccgg
    33541 tatttgaagc gatacttaaa ctattggcca ggacgcagtc atggtgggat cggcccgctc
    33601 cacgcgccct gctcgaacgc ctggccgcgc cggtgatgaa gcgcatcgcc tccacagcca
    33661 aacttccggc ggacatggtc ctgcccaagc tcgcgacgac tcatttgcgc aatcggtatc
    33721 cggagctcac gcaaccgtcg gcgaaaaagg ctggccctcg cgtcgcctat ttccatggct
    33781 gcgcggcgaa ctacttcgac gatggggtgg gggacgcggt catttccgtc ttacgcaaac
    33841 atcacgtaga gccggagctt ccaccgcagc gttgttccgg gacgccgatc gaaacctacg
    33901 ggcatgtaga tttggtgaaa gacaacgccc gcttcaatct gcaatccctg gcgggctatg
    33961 atcaggtcgt cacgggctgc gcctcctgca cgctgatgct caaggaatac aaaaagtttt
    34021 tcgagagcgg gccggaacag caggcggcgg atgtgttggc gaagaaggtg gtccacatca
    34081 cggaattcgt ggcgcgatcc gggcagcatc caccgatggg gacgccggaa ggccagggcg
    34141 ggaaggtcac ctaccattcg tcctgtcacc tgcgagccgc cggggtgacc aaagaaccgc
    34201 ggcagttgct caaacagctg ccgggatcgg actatgtgga gatgaccgac gccgatcgtt
    34261 gtgcgggcgg cgccggcacc tacatcgtga aggactacga cacgtcgcag aaggtcttcg
    34321 accggaagcg gcggaatatc gagcagagcg gggcggagac ggtggctacc agttgcccgg
    34381 cctgcatgat tcagctcagc aacggcatgc gcggccgcgt ggccgtgaaa catgtggcgc
    34441 aattactcaa cgacgcctat gaggcggggg ctgcaacaga gacccgacca ggagccggcc
    34501 aatgaccttt cgcaaagtgg atatcatttt tgtggtgctg gcgctgctcg tggtcggagg
    34561 cgtggccctg ctgccgtcgc cgcgcgaccg caacccgaag gtgccggcca acagcgggca
    34621 tcaggccgtg acgatggaaa aggattgtct ggcctgccat gtgccgaccg gcgctcgtcc
    34681 cttaccggtg cagcatccca agcggcagga ttgcctgcat tgccacgcgc gggcgcaggg
    34741 ataaggcgta aaacgtgaaa cgttagacgt gaaacgggaa acgtaaaagg cgaagaaata
    34801 ggacgtaaga ggcaacgcat aagacggaac aggaccgaaa gtcgtttcac cttttacgtc
    34861 tcacgtttca cgcaccgact gaacaggagg attcatcgac tatggtcaaa gtgttgattc
    34921 ttgggccgac gttacaacag cgggtgacgg aaccggaatt ggatgtggaa gtggaaggat
    34981 cggtgcccat caagctactc atcgaaggca atgccgatct catggatgcc ctggcgccgt
    35041 tcgtggtgcg cggcgaattg ctcgtgaccg tgaaccgcaa ggtcggctca ctcgattccg
    35101 tggtgaaaga cggcgatgtc ttaaaaatct cccaccaatc ccgcgcctcc tacgacggca
    35161 ccaccgatat tcctacttga gtaggcgcct ccgggaagag gcgcaagagc gtatggctga
    35221 tagcatatag tcgggaggag ccacttccta ccatcggcca ttagccattt gctcttctcc
    35281 ctctccgacg ctcttctctc cctcgggcca tcctggtaga ctggcgccgc aatcgaccgg
    35341 gccgggagtg gggagacgat ggcgcgcaac gatcaagcgg tgcgtctgct ggtggtgttg
    35401 aagcagctgg aggcgtcgcg gcggggcctc acgctggagc agctggctga gtcgctcgcg
    35461 ccaggctcca ctcgccatcc tcgcactctc cggcgcgatc ttgctgcgct ggaagaagcc
    35521 ggttaccccc tcgtcaccga acgcatcaac ggtcagacct gctggcgtct gatggaggga
    35581 tttcggaacg tccccggcct gcgcttctct ccctctgagc tgatggcgct gaccttcagc
    35641 cgccggctca ttaccccgct ggaaggtacc gaactccata cctctctgca gtctgcctta
    35701 gggaaagccg ccgccgcgct gccgccgcag ggcgtggccc tggtccaaca gctcgacggc
    35761 accttcagcg tgggcctcgg tccgcacaag cgctatcgac agcaccgcga aaccatcgac
    35821 cgcctgacca gggcgatcgc cgatgccacc accgtgcaga tccgttacga ttccgcctca
    35881 cgcggccgta cgaccaggcg cgaagtggat ccctatcggc tctggtacgc gaccggcggc
    35941 ctctacctca ttgcctactg ccacctgcga cgcgagccgc gcatgttcgc cgtggagcga
    36001 atcaagtccg tcacgcccac cgaccatccc tatcagatgc cgctgcattt cgatctcgac
    36061 gccttcgtgc aggatgcgct gaccgtcatg cgcgggccgc gcatcgaggt ggagttggaa
    36121 ttcaataagg ctaccgccgc ctgggtgaag gatcgagtct ggcacgctac gcaggaaacc
    36181 aaacgaacca aaggcggagg cctgcggatg accctctccg tggccgatac ccgagaactg
    36241 gtcgggtggg tgctgagctt cggcagcggg gtgaaggtcc tcaagccgga gagtctgcac
    36301 caagccgtcg tcgaggaagc ccgccgcatc agtcaggcat agcctgcgta tatcatcttt
    36361 ccaatcttct aggaatctcc ggcctgcact gttctcatga accattcgtc ctcgatcagc
    36421 tgcgcactgg tgattgacgt catgccttga cttctcagtc acctcgccag acaatgcgta
    36481 tgttgaagaa ttcattaact gttgggaata ggaggttgcg gtggcgacgg tgaattttag
    36541 tgtcccggag gatgtgaaaa agagattcga cagggcattt gcgggtcaga ataaaagccg
    36601 ggtgattgcc gatctcatgg ctcaggcggt tgaggaacag ctcatgttga aacgccggag
    36661 tgacgcgatc gactctctat taagtcggcg acgaaccaaa cgaccagtag ccgcacgcgc
    36721 tattcgacag gctcgggaga gcggtcggca atgacacggg tcgtcgttga tgccagtgtg
    36781 gttgtgaaat ggctattgcc tcaacgagag gacgaggccg acgtcgaaca ggcgttgatg
    36841 ctgctcgatg ggttgagaag cggccgggtg aagatctttc aaccagtgca ctggctcgct
    36901 gaaacggcgg cagtattggt tcgactcagc ccggagaccg ccgctgccga tgtcgcagat
    36961 ctctacgcaa tgagtctacc tatagaaagt gggccagagg tttatcttac cgcatgtgag
    37021 ttggcgcaca cgctagacca ccatgttttc gatacgttgt atcacgcagt ggcgttgaca
    37081 ctgccggata cggtcttggt gacggcggat gcgcgctatg aacggaccgc ccggcgaagg
    37141 ggggcgatca tgttgttgcg cgatgtggtg tcggtgtagt caatctgtgc gttccaccgt
    37201 gtgcggtgtg atggtggttt cagggttgca ttgccgacag cagaaaagtt gtcggctggg
    37261 tgctgagctt cggcagcggg gtgaaggtcc taaaaccaga cagtctgcgg gaagcggtga
    37321 gtcaggaagc gcaacgaatc gcagagcaag gggagtgagg gggcgaggaa cccacggacg
    37381 cagagtgaac gcacatgcgt cgggtaggcg gatctaggca gcaggcaacg gggaaatgtg
    37441 attgatttgg gccttgatat tccgcgccgc ttcccagaga tcaaccgcac gctgggcgtt
    37501 gagccagaac tccgggctgt tcccaaagag tcgtgaaagc cgtagcgcca tctcaggact
    37561 gaccgcccgc cgctcctgca gcaattcgtt caaggtctgg cgggaaaccc ggatcgctct
    37621 ggcacagccg gatacggtca agccatactc cggcaggaaa tcttcgcgca gcatcgcccc
    37681 cggatgagta ggccgaactt tgcgtgctcg cttattgggg atgctcatga cacacctctg
    37741 ctcgtggtag tctgtgatct cgacgtcaaa ggcggcgccg gattcaaagc ggaagcatat
    37801 ccgccattga tcgttcaccg aaatcgaata ctgcccagcc cgatcgtgct ccaatctgtg
    37861 cagccgatta ctcggaggaa ccttcaagtc tgacacactc atcgcaagct ctagatactc
    37921 taacttgcgc aatgctcgtt cccacatatc gggtggaaag cgctttgact taccggtgtc
    37981 atacagatcc ttcgtgccct tgtcggcaaa gcttttgatc acgtgtgaag tgtaacgcga
    38041 tgcgtgacac cagtcaacct gctatcaatc ggtcgcatgc ccttgtttcc ctacgagcgc
    38101 tgtctccctt gccgcctctc gttcctccac aagcgacgtt tcacgattcg cgagatacgg
    38161 cctcccggga ctcccgcgag atacgcttca cgcgctatgc aatactcgcg tcgatggggt
    38221 tatgggtcgt cgagtcctgc ggcatgtatt gacgatatgt cagatatgac atatcgttcg
    38281 gttccaggac ggcagagtgt atgactgatg ggcgggatgg acaaactact cgtatggctg
    38341 aggggagagg tcaagactcc gccttttccc caagccgcgc gactcgaagc gggctatctg
    38401 ctgaggtcat tgcaagaggg agagtccctc ggccttccgc attcacgacc catgccggtc
    38461 attggcgtac gatgccatga gttgcggatc attgatgaga gctgcacgtt tcgcatcacc
    38521 taccgagtag atgctgatgc agttgtcatc ctcgatgtgt ttaagaagaa gactcaacag
    38581 acgcggagtc tctcattgac acctgccggc gtcggctcag agagtatgac cgagcgatgg
    38641 gatcgtagga ggacttcaaa tgaagaacac aaaacgagcc aagctggaag cggccggatg
    38701 ggctgtcggg tcggtgaagg agttcctggg cctgtccgag gcagagtctg cgctcatcga
    38761 tatgaagtta gcgctcagtc ggagtctgcg agatcgacgg aataaacagg gtttatcaca
    38821 agtccaactc gccgaacgcc ttcaatccag ccaatccagg gtggcaaaga tggaagccgg
    38881 cgacccctcc gtgtccatgg acctcttggt cagctcgctc ctgctcctcg gcgccagctc
    38941 cgccgatctt gccaaagcga tcagcggcga agggcgcgga aaaatttggc gagcagcgta
    39001 gttctttctt tcgacgggtt gccccatgga gtaaggccat gaagcctgct tctcacgccg
    39061 ttctcctgcc atctgtcata ggctcttaat tcccgcaagc cattggccat cagctcttct
    39121 ctccgataga gatacgcatc aggagtgacg agatacggct ctttaagccc tacaagcgac
    39181 aagcgtcggc atgtgaccgc gggcatgact ggtgttcctg gcgggttttg gctacactat
    39241 caccaggaga gacgtgatga gtatccgcga attgcttcgc tcaaaacgag ctgagattct
    39301 ggagattgcc gcgcggcatg gtgcgcggaa ggtgagagtg tttggatcgg tcgcgcgtgg
    39361 aaccgctcgt ccgagcagtg acatcgactt cctcgttgag atggaggaag gtcgaagcct
    39421 gctgaccatg cggcactgat cctcgatctg gagcgattgc tgaaacggtc ggtcgatgtg
    39481 gcctccgaac gcgggcttcg acgtgtggtt cgaacagagg tcttgaaaga cgcgatcgcg
    39541 ttatgaagga tgatcgaaac cgctctctat gtccctcggc ccgcgtctga catctcacgt
    39601 gccattgctg gcgttctagg caggaattgg ctagactacc tcgcgatgcc tgaactctgc
    39661 cgttttttgg ggatgatcat cacgatgtat tacgacgatc atgcaccgcc tcattttcac
    39721 attcgctatg gggattacaa ggcaatcatg ggcatcgatt ctctcatgct cttggatgga
    39781 tacctgcctc cacgggcatt gggactggtt gcggagtggg gcgccttaca ccgggatgaa
    39841 ttgtgggaag actggtcatt ggccgaacag cgagcgccgc tgaaaaaaat taaaccgctg
    39901 gagtaagagc catgctcaaa gaccttgtcg aagccgcgcc tcttgacggg tatcgagtgc
    39961 ggctgcgatt tgaagacggc atcgagggcg agctggacct agccacaatc attcactttg
    40021 agggtgtctt cgctccactc aaggacctgg ttcggttcag agaactcgca gtccatccag
    40081 atctggggac catttactgg cccaacggtg ccgatctcga tccggttgtg ctctacacgc
    40141 ggatcaccgg ggcgccgctt cccaagtacg aagccaaagc gatccactga ctcccatggc
    40201 ctacctgtcg ctagtctgtg gaggctccta tccgtctccc cgtttcccaa tttagcaccc
    40261 atcactcaac tgcaacacct ttcatgtccc tccgttcctg atccgaacag tgctctgttg
    40321 ctttcatctt tttcccgtct cgccccccat tatgaccccg gacgtcacgg caggggagta
    40381 tgctgtcggc atgacgtaca cagacagaag ggaggcaatg atgcgacacg cagcactgat
    40441 cggcgcattc ctgctgatgg caatgaccgg gacagggcgg gcagagcccg gcttcgacga
    40501 gaagtaccag cgcgacttca acatcttcaa tcccatcaac caataccagc caggcaaccc
    40561 gctgaatcct atcaacgcct acgacccggc gaatccattt aatccaatca accggttcga
    40621 tcccggcaac cccgccaatc cgatcaacca gttcaacccc aacaatccgt tcaatccggt
    40681 gaatcgctat cggccggaga atccgttgaa tccgatcaat gagttcaatc ccaacgtgcc
    40741 gtttgcgccg ttggatggtg ggggagggat ggggaagagg cggtgaggcg acacatgcct
    40801 ttcccgatta cagtgaacga cgattcacga accactgcca tcggctatca gccatcagct
    40861 cctgttccga tcgagatacg cttcacgaga tacgatcttc gaaggtgatg atttcttgag
    40921 gggaacaggg tattctgcgg cggcactgtc ggcgagaagt agaaggacga gagcatgccc
    40981 aagaaaacca agcctgtaac acagaaaaat cagaccgccc cctacaaaca tccagaggcc
    41041 aagagcctca tgcggccaga ggtcggcacg caggcgcagt tcaagaagaa gaaaccggcc
    41101 aagacctacc gctatgattc gtcgctgtcg cccgggctcg attgggacgc cacgaatcca
    41161 gcccgtgagg aaggcgaggc gctcatcaag aaagtcctcg acgccaagac gctcgaagaa
    41221 gccaaagccg ccgcgtctaa actcaagagc ctcagtatgc cgttcttgaa ctgggcgggt
    41281 aaagcagagc ggttgtcctt cgatgtaccg accttaccgc tcttcatcca tgagcggctc
    41341 tccaccaaag ccatcattga aacgctagcc gggcataaga ccgacaagca agaggatatg
    41401 ttcgcactgt tcggcgatcc acagcactct attaccgatc aagtattgaa atcctacgag
    41461 taccaagacg attggacgaa ccgaatggtc ctaggcgatt cgctcgtcgt gatgaactcg
    41521 ctgctgcatt atgaaggttt gggcggtcag gtgcagatga tttacatgga cccgccatat
    41581 ggtgtgaagt ttgggtcgaa ctttcagccc ttcgtccgca agcgcgacgt gagccacaac
    41641 gacgacgagg acatgacccg cgagccggaa atggtccagg cttaccggga tacctgggag
    41701 ctgggtctgc attcttacct gacgtatctg cgagaccgct tgcttcttgc gcgggatctg
    41761 ctcacaccaa gcggcagtat ctttgtccag atcagcgacg agaatctgca tcatgtgcgg
    41821 gaagtaatgg atgaagtgtt tggaaaagaa aatggctgct gcgtcattgc tttcaaaaaa
    41881 acctcttctg taagtagtcc aatggcgcgt gtcaatgtcc ttggcacagt atccgattac
    41941 cttctgtggt acgcaaaaga catagaacga gttaagtata ggcagttata tcagacaaag
    42001 aacttggaat cagatgtggc gggggtttac tcgagaatcg cttctcctga cggtaaggaa
    42061 atgcgaagaa tgtctccggc tgagttgtca ggtgaagttt ccattccaaa aggctggaag
    42121 gtgttcagaa tagataacat gacctctcaa gggcatagcg aggtgttatc tcagcctttt
    42181 attatgaaag gccagtcata tttccctggc aacaaccgtc actgggctac cacctcggag
    42241 aacttaaagc gtcttgaggc aatgggaaga cttgtacctg caggtaagag cctggggtac
    42301 gtgcggtttc taaatgactt ccccgtttat cccatcgtaa atatttggac tgacacaggg
    42361 accggaagtt tcacagagga caagctttac gtagttcaga cagcgacaaa agttgttgag
    42421 cggtgcctgc ttatgactag tgaccctggt gatttagtga tggacccaac ctgtggaagt
    42481 gggactacgg cttatgttgc agaacggtgg ggacggcgct ggatcacggc tgacacaagc
    42541 cgcgtaccgt tagccttggc tcgtcaacga cttctcacgg cgacatttcc ttggtacgaa
    42601 ctcaaggaag aggcaagagg gccagcggga ggattcacat acaagcggaa gcaaaacaag
    42661 tcagaagagg aagttggcgg cattgttccg cacatcacgt tgaagtctat cgccaacaac
    42721 gaaccgccag ccgaagaagt gctggtggat cggccagaag tggagaatgg catcacccgc
    42781 gtgactggcg cgttctgttt cgaagcgacg atcccgacgc cggttgattg ggaaggcgac
    42841 ggtattgaag attctggagt tgtcccaggt gaagcatacg gttcatttgt cgatcgcatg
    42901 ctcgaagtgc ttcgcaagag tccggtcatt cgattggaag gcaacaagac tgtcacgttt
    42961 aagaatgtca ggcctcccgc aaagtcgctc tcactctctg ccgaggcatt ggtcgtcaat
    43021 ggccaggaaa aggccgtagc catggtcttt ggtcctgaga atggtgcagt cagtgagaag
    43081 ctggtctacg aagcagcacg cgaggcgcac gcgaagaact atacccatct ctacgtgatc
    43141 gggtttgcga tccagccgaa tgctcggacg ctcgtggaaa aatgtgcgga tgtgatgggc
    43201 gtccccgcaa cctatgtcca ggccacgccg gacttgatga tgggcgacct actcaagaac
    43261 atgcggtcga gccaaatttt cagcgtctgt gggcaaccag agattaaggt tattcggact
    43321 aaggataaac aataccaagt tgagctactc ggtctggatg tgttcgatcc cattacgatg
    43381 gacgtgacgc accgaagcgg taacgatgta ccggcctggc tccttgatac ggactacaac
    43441 gatctctgtt ttcatgtctc gcaagccttc ttccctcgta ccaacgcctg ggacaatctg
    43501 aagaaagccc tcaagggcga atatgaagag tctgtgtggg atcacttgtc cggcaccatc
    43561 agcgccccat tcgaggcggg cgagcacaag cagattgccg tcaaagtgat cgacgatcgc
    43621 gggaatgaac tgctggtcgt gaagaagctc aatgggacta gccgatgatt gaacgggtca
    43681 ccatccggcg attcaaacga tttcaggatg tgaggtttga tctccccggg cacattgtgc
    43741 tggccgggcc gaacaattgc ggcaagacca caatgcttca ggctattgcc gcctgggatt
    43801 tagccttaac tcgctggaag cagctccatg actttcagcg ccatcgcggc ttctatacca
    43861 aagccccaat cgccaggcag gcgttcactg ccgtcccact ccgtgctttt gacctgctgt
    43921 ggaataaccg gtcctattca ggctccatcg agatcgaaat ccgccactcc aagggttggt
    43981 gggtcacgat ggagttgatg gccgacagta cggagcaagt ctatgtccgt cccaagcccg
    44041 atgctgatcc tgaaatggtg cggctggctg aactgaaaac ggtctttgtg cctcccatga
    44101 cgggtctgag agcactgccg agccagtcta tcggcgaccg aaggtggatc aactcctcgg
    44161 acagggcaag cccggtgaag tgattcgcaa tctgctggcc gaggcgtttc agtcttcagc
    44221 ctggtcgaag ttggaagatt cgattcgccg cctcttcaat tatgaaattc ttcctccgga
    44281 ttctaccggg gccgacatca ttgcggaata tcgatttcag cccgatgggc ctcggctcga
    44341 cattgccagt gctggcagtg gatttcagca ggtcttaatg ctgctgacct ttctgcatgc
    44401 ccgaccgggg gccaccctgt tgcttgatga acccgatgca catttgcatg tgattctcca
    44461 agacgccatc tatggcgaac tccgatcggt ggctgccaaa acaagctctc agttaatcat
    44521 ctctacgcac tctgaggtga tcattaactc tgtggactcc cgagagctgt gcatgctgat
    44581 caaccatccg cgattgctcg aaagtacggt tgagcgaagc cggctgacag attctctcgg
    44641 cattctgacc cacaccgaca tcatgattgc tcaggatgcc ccgggcattc tttatgttga
    44701 gggatacacg gatgtgaatc ttcttattga gtgggccaag accctcggcc acccggcgta
    44761 tcagacgctg acgaccaaac tattctggag gccttcgatc tgggagcctc gtccatgggc
    44821 tcacggcgta aaagcagagg accattatca agctattcag cttgttcgtc ctggactgcc
    44881 gggactgata ttgctcgatg gagatgacaa ctccagggtt ccggagactc caatcactgg
    44941 gcaaggactg caacggctcc gctggaagcg gtatgagatc gaaagctatc ttattcaccc
    45001 agcaacatta gagcgttttg tggaaaagga agttgggact ggggcgcaat ctgttgaggc
    45061 aaagaaggat atgaacgctt acctagaagg aacattcact cccgcgtttc ttcaggaccc
    45121 gctgaagcca aatcccctgg tggatgccta cctgcagcag cgcaaagcga gaaccgatgt
    45181 gctccctccg atccttgccg ccgccggact gcatgccttc ccctatactc gtttcaatga
    45241 gatcgcttct gtcatgctac cggaagagat tcaccctgaa gtcaaagaga agctcgacgc
    45301 aatccagcgc gcgtttggtc tatgagcgcc tacgaagttc cacagcccat tctgaacgcc
    45361 cccttcgaag agccgaaaga gcattggcat atcgttgaag gcgaagagcc cgagcaacgg
    45421 cctggccgtc gccccgcgat gtatttctat cgcgatccca aagtgaagcc tgaaaaggaa
    45481 tatgggacag tcgccgggac tgccattgag ttgaagctgg taaatcgcat ccgggcccaa
    45541 gtgaagaagt ggcgggcgga tggttatccc ggtgtaacga gaacgactct tgagttgctg
    45601 caatggtggc gccgggaggg acgggcacaa cgactctttt tcgcgcaact cgatgccgtg
    45661 gaaacgatta tctttttgtg tgaggctcga gctgattttc gccagggtat cgaggtgccg
    45721 caagaggaac tcagcgagga caagcgcgca cagggtttta ttggatttcg gcggtatgcc
    45781 tccaaaatgg cgacagggtc tggcaagacg accgtcatgg gcatgctggc agcctggagc
    45841 attctcaata aggtgaatga tcgaggtgat gcgagattct ctgatgtggt gctggttgtg
    45901 tgccccaatg tgaccattaa gagccggctt cgtgagttag accccgaagc gggggaagtc
    45961 agtctctacc gcacgcgcga cctggttcca tctcatttga tgccgctcct gacacgaggg
    46021 cgggtacttg ttacaaactg gcacgtgttt gagcctcaag gcatccagac aggtggtacc
    46081 ggttcgagag tcaataaagc gggcgttgag ttgcgcacca aggagacact gatcatcggt
    46141 cccaagacga caacggcgcg tggaaaacgc tatctgaccc ttgaggattt tgaacgtcag
    46201 gttgctgccg ggatgctgac ggtcttagga gaggaacgag agaatgatgg cagccttaag
    46261 aaggtcagcg ttgaatcgag gcgatatgtc gaaagcgaca cggctctcgt caaccgtatt
    46321 ctgggacgag aggtaggcgg caaacagaat attcttgtga tgaatgatga agcgcatcat
    46381 gcctatcgca ttgtgcgtga tgttcgcgat gaggatgaag aagatctttt tggggaagat
    46441 gaagaagccg aagacttctt caaggaagct accgtttggg ttgatggact ggatcgtatt
    46501 cagaagttgc gaggaatcaa tctttgtctg gatctgtccg ctacacccta tttcctcggg
    46561 cgagtcggcc aggatgctaa ccggcctttc ccctgggttg tgagtgattt tggcctgatt
    46621 gacgcgatcg aatcagggct agtgaagatt ccgcagcttg ccgtgcgtga caccaccggg
    46681 aaggaaatcc ccggttattt caatatttgg cattggattc ttcctcaact cacacccgct
    46741 gaacgaggtg gcaagaaggc cagccccaag cctgaggcga ttttgaagta cgcccatcac
    46801 ccgatcgcga tgcttggagg gctctgggaa aaagaacggg aagactggat tgccaatcga
    46861 gaagatccgc ggccaccggt gtttattctc gtgtgcaaga atactcaaat cgccagggtg
    46921 ctttacgagt ggttggctga agatacggcc ccaactggga ttcctcctgt gaagattgag
    46981 ggtttcagaa ataatgggct gcagcacacg attagggtgg attcaaaggt agtgcgggaa
    47041 tcggattctg gtgaatccaa gagtgatgaa gtgcgctgga tgcgcttcat gctggatacg
    47101 gttggtaaac aaacgtggcc gactgatcga gtcggacggc cgatttttcc ggaaggcttc
    47161 aaggagttga cggacaagct agggcgtccc gatcaccctc ccggccgaga tgtgcggtgt
    47221 attgtgagcg tgggcatgct gacggaaggt tgggattgca atactgtcac ccatattatt
    47281 gggcttcgtc cttttatgtc gcagctccta tgcgagcaag tggttggtcg aggcttacgt
    47341 cgagcgagct atgagcttgg ccctgatgac agactgacag aggaagtagc gaaggtgttc
    47401 ggtgtaccgt tcgaggtgat tccgttcaag tcctcgccac aaggccaacc taaaccgagg
    47461 gtcaagcggt tccacgtgca tgccattccc agtaaggccc aatatcaaat caagtttccg
    47521 cgcgttgaag gatacactca ggccattcgc aatagggtga cggtggattg ggacagcgtg
    47581 ccgtcgttgg tcttggaacc agggcgtata ccgcctgagg tcgaggtgaa aggcttgcat
    47641 gtcaatacca agggtaagtt atcgctcagt ggacctggac ggattgatga agtgaatctt
    47701 gatgagttcc gcgcccggcg acgtttgcaa gaactggtat acgatcttgc gaagactctg
    47761 acccacagtt acgtatctca gaagaactgt ggcgtagctg cacatgtcct gttcccgcag
    47821 gtagccacta tcattcaccg gtatcttgaa aagaaaattg acgttcgctc tcccgccaac
    47881 ataaaagatt tgtttcttgc gccgtattat gggtgggtcg ttgagcgcct tcttgaggcc
    47941 attcggcccg acacctccca aggagagagt cctgaggtcc cacgatatga aacaagtcga
    48001 ggttcggggt caacgggcga ggtggatttt tggacaagcc gcgacgtgcg cgaggttgga
    48061 aagagtcact tgaattatgt agtcgcagac acggctcagt gggagcagtc tgctgcgtac
    48121 taccttgaca aacatccgaa ggtggacgcg tttgtgaaaa actccggact tggcttcgcg
    48181 attccatatc tcgacaatgg ccagatgcat gactatgtgc ctgatttcat tgtgcgatta
    48241 aagcatgcgc cgagccatca tctcatactg gagatcaagg ggtttgatcc gagagaggat
    48301 gttaaacgag cggcggcaga gagatgggtg gcggcagtta acgcggatgg agctcatgga
    48361 acatgggggt acgctatagc gaagaaagtc gccgatattc gacccctgct ggaggagcat
    48421 tcacgttgat gaagaaatgt ggaaaagaaa tgatgtcgag aaccattttg cctcgtgtaa
    48481 gttgagttag atgaggcgac gaccaatgta caaggtatgc acattgccgg ttccgtaagg
    48541 ctggatcact tgttgattcc ggcaatggaa atggtgccag gactcatttg ccaaggatgg
    48601 aaatcatttc tggcaccacc tctcgtccca atcctcatct aatttccttc attaaattta
    48661 ccctctcagc gcattctaca ataatgccca tgcatatttg agtttgatgg tctccatgcg
    48721 tagacgcctt cccgaaacct gtttcaagac aagatctgct gtcgtagaat agggtcagca
    48781 actgtcttga aaatcaagcc gactgactga ccgcgacccg cca
//
LOCUS       DAORRQ010000030        47905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_44_length_47905_cov_33.059352, whole genome shotgun sequence.
ACCESSION   DAORRQ010000030 DAORRQ010000000
VERSION     DAORRQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 47905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47905
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44_length_47905_cov_33.059352"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..1450
                     /locus_tag="PK224_10930"
     CDS             416..1450
                     /locus_tag="PK224_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB16295.1"
                     /translation="MPENNHSNAPLPPPHVQLIQMAMAHWVSRIAYVAAKLGLADHLA
                     SGPKTADDLAGPTGTHAPSLYRLMRALTNLNILTEDATHRFALTPLGEAVKTGAPGSA
                     RPSILTLASDWMSRGWEQLLYSVQTGGCGFEQSLGMPLFDWLAKHPEDASLFSETMMG
                     FHGAEPAAITAAYDFSGLSTLIDVGGATGHLLTTILTGNPASRGILYDLPHVVRDAPA
                     LIQARGLADRVTIEAGSFFDRVPGGGDAYLLSHIIHDWTESQCLTILGHCRRAMTPGS
                     RLLIIEMVLPSDNAPHPGKMLDMMMLVGPGGQERTESEYRELLGKAGFRLTRLVPTET
                     AVSVVEAVLA"
     gene            complement(1542..2717)
                     /locus_tag="PK224_10935"
     CDS             complement(1542..2717)
                     /locus_tag="PK224_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRB16296.1"
                     /translation="MKPYRLFAFTLGLLLSCSTLASAEILALLNYESKPDQPVRREGI
                     AIMDIDPESGNFGKILMEIPLPPDLVAHHIFFNRDRSKAYITALGKSILHVVNLRTFP
                     YRLQAIDVPDCQMGEDLAVSEDNRTWYLTCMGSDNVIMGDALLDKPIKTVSAAKPSLA
                     TIQYPHGIAIHNGIDRLLVTSTVKPDMSDAGDSITVLEAASGKVLSTHKVSSKPSPAK
                     AAPVEVMFSPNANPPVVHITNMLEGTLWAGVWDPASRTFSFHQVDDFGSRQQGMPLEM
                     LYNAKGDRLFVTTAKPGFVNLYDNSDPRQPKFLKTIAAAGGAHHSVLSPDERYLFVQN
                     SFLNLDGMNDGSITVIDLKADKVLGNIDTLKAQGFNPNCIMLLPNQPGDLRASRIID"
     gene            complement(2820..3164)
                     /locus_tag="PK224_10940"
     CDS             complement(2820..3164)
                     /locus_tag="PK224_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRB16297.1"
                     /translation="MTNATTTEMDQTTGLYDMTNLEKMKDLWTHAPEAMKAFVAFDKA
                     ALAAGAIPVKYKELMAMAVAFTTQCPYCIELHTNKAREYGASDPEIAESVLVAAALRA
                     GGAITHGTHAMK"
     gene            complement(3196..4017)
                     /locus_tag="PK224_10945"
     CDS             complement(3196..4017)
                     /locus_tag="PK224_10945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB16298.1"
                     /translation="MTTNTVVPEMDGLKTRLNTIWTAGDYDRFSRYMEGGAREFYERL
                     QIAPGCRLLDVGCGSGQLALLAAKDGVEVTGVDIAANWVERAQARARAEGVTARFEVA
                     DAEALPFEAASFDVVVSLIGAMFAPRPDLVAQELLRVCTPGGTLAMANWTAEGFVGQM
                     FKNVAKFIGPSGMPSPVLWGNEATVQERLGHGLSTLSLIRRQYLFSYPFPPSEVVEFF
                     RLYYGPTNRAFASLDAEGQAHLRRDLEALWTAHNQAGPDCTTVYGEYLEVIGIRA"
     gene            4215..5240
                     /locus_tag="PK224_10950"
     CDS             4215..5240
                     /locus_tag="PK224_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRB16299.1"
                     /translation="MDVLSEVLKAVKLDGAVFFHGEFAAPWCAREPDAGTMASYLPTQ
                     AGHIIIFHLLLEGRGYVRLEQEDRAVPLTAGDIIVLTHGDAHFMGNGPPVTPVDTSQQ
                     IRQILSEGRMLSQLAGGGEVTKLICGYLTCDAQLCRVVLAGLPAMLKVNIRDTPSGQW
                     LENTLRYSVDHAEASGPGGAAVIAKLSEALFVETLRRYIAQLPHTQTGWLAGVRDPDV
                     GKALALLHQQPARPWTIAALAAEVGVSRSVLAERFQHFLADTPIGYLTRWRLHLAAQL
                     LTSTSKSVAEVAGDIGYESEPSFNRAFKREFGLPPARFRTQAKSPKQPSVGRESTPAE
                     LKPIQTT"
     gene            complement(5623..6069)
                     /locus_tag="PK224_10955"
     CDS             complement(5623..6069)
                     /locus_tag="PK224_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRB16300.1"
                     /translation="MSKNTVRLHRVFRTTPERLYRAFLDADAMAKWLPPNGFTGKVHQ
                     LDAKVGGTYRMSFTNFTTGQSHSFGGKYLELVPHERLRYTDTFDDPNLPGEIQVSITL
                     KKVSCGTEMTVVQEGIPEVIPVESCYLGWQESLILLAKLVEAEIPE"
     gene            6531..7220
                     /locus_tag="PK224_10960"
     CDS             6531..7220
                     /locus_tag="PK224_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HRB16301.1"
                     /translation="MHCSNPLGSRRLVSFSLACALVLFGLIGSGCQTAYYETMEKLGY
                     HKRDLMVSDVKKARDAQQDAKEQFKSALDRFTKTLNIQGGELQDKYDALNEEYQQSEK
                     RAQAVRDRIASVENVSDALFDEWTAELKQYSNATLRQKSQKQLTQTRSQYAQLIKAMK
                     RAEAKMDPVLAKLKDHVLFLKHNLNAQAIASLKSELTTVEGNIDSLIKDLNASIQEAD
                     SFIASMEKDNA"
     gene            complement(7310..8206)
                     /locus_tag="PK224_10965"
     CDS             complement(7310..8206)
                     /locus_tag="PK224_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRB16302.1"
                     /translation="MSTPDLNLLITLDVLLAEGSVARAATRLRLSPSAMSRALARLRE
                     TTGDPLLVRAGRGLVPTPRAVELRERVSQVVEEGQAVLRPAEKLNLTQLVRTFTLRTS
                     EGFVENFGPDLIALVGEEAPGVRLHFLQKANKDSAPLRDGTVDLETGVIEKTTAQELR
                     VQALFRDRLIGVVRTGHPLSKGKITPSRYAGGKHISVSRQGLEKGPIEEALQRLGLER
                     DIVTIVGGFATAIALARASDLIATVPERHTGILRAGMHSFSLPVSTPEFTVSLLWHPR
                     LDADLAHRWLRGHVRQICAAIR"
     gene            8375..9013
                     /gene="tmpT"
                     /locus_tag="PK224_10970"
     CDS             8375..9013
                     /gene="tmpT"
                     /locus_tag="PK224_10970"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HRB16303.1"
                     /translation="MEPQFWHDRWTKNEIGFHKSDANPLLVKYFTELALPKGSLVLVP
                     LCGKTLDISWLLSNGYRVAGAELSSNAIEQLFAELGVKPTITGNGEIDRYHANDIDIF
                     VGDIFDVTRTMLGPVDAIYDRAALVALPENMRSRYTAHLTAITDHAPQLLVCYEYDQR
                     AAEGPPFSISHEEVRRHYGERYHLTLLTSADVPGGLKGKCSATEHVWLLRRG"
     gene            9016..10482
                     /locus_tag="PK224_10975"
     CDS             9016..10482
                     /locus_tag="PK224_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB16304.1"
                     /translation="MITQFMAGRDEAQAGTTDLAPSIRWILAGLSLSMLLSSIGTSIA
                     NVSLPTLAQAFDASFQQVQWVVIAYLVTITTVIVSVGRLGDMTGRRRLLLVGVFLFTV
                     ASVLCGVAPTLWLMIAARAAQGLGAAIMMALTMAFVGETVPKSQVGSAMGLLGTMSAI
                     GTALGPSLGGMLISEFSWRAIFLINLPLGLLIFLLAYRFLPVDRPRQQVDRLGFDHAG
                     TLLLALTLAAYTLAMTIGRGSFGPRNLALLVATLLGASLFLFAETRAESPLIRLTMFR
                     NPLLSASLIMSALVSTVMMATLVVGPFYLSRTLGLHAALIGLVLSVGPLCAALTGVPA
                     GRLADRLGPQRMTVLGLIGIAAGAFLLSVIPATLGIYGYTAPLIVITVGYALFQTANN
                     TAVMTDTRPEQRGVISGLLNLSRNLGLITGASVMGAVFAAASSTIDITTAPPAAVAIG
                     MQVTFAVAAVLILVALAIAVATYRRELYGWAVAPTAES"
     gene            10721..12160
                     /gene="pyk"
                     /locus_tag="PK224_10980"
     CDS             10721..12160
                     /gene="pyk"
                     /locus_tag="PK224_10980"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRB16305.1"
                     /translation="MQKTRIICTIGPATESYEMLHKLYEAGMSIARLNMSHGDHESHA
                     KVIQHIKSLNRKVKFPIPILLDTQGPEIRTGDLSNELDLRQGDIVSVTTRGPMNVEES
                     SIHINYADLLEAVNVGDRITVDNGLINFEVLEKHERHMRCRVLDGGLLKSKRHVNLPG
                     VRVNLPSITQKDIKDILFGLERDVDFIALSFVREAGDIQQLKDLMGDKVGKVKIVAKI
                     EDQEGVRNLEAIIKESDGIMVARGDLGVEINLEDLPNVQRTIVRLCAEYGKRVIVATH
                     LLESMIHNPHPTRAEVTDVANAIYEEADAVMLSGETTVGKYPVKCVEFLRRIALKSET
                     IPGLQFAKHLRNAENKQQLAAAAVQLAEGVKAKGIVVITRRGLMADLVANCRPFATNI
                     YAFTNMSQPRRTMMLNRGVLPFKIDFSSDPEKTLQTAFRILKDREDFKVGDKVVIISD
                     VLAQQRVDSIQIRDVPSDDILPDLSAVSH"
     gene            complement(12446..12904)
                     /locus_tag="PK224_10985"
     CDS             complement(12446..12904)
                     /locus_tag="PK224_10985"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB16306.1"
                     /translation="MPITMQQVLAEIDKDEPNYEALALLGPEALPHLQLIAEADDPLK
                     SSKAVYAASLIGGAGAVEVLRKAADHHDPQVRIAVAQGLQNLAAVAPTDLVMKSLNDH
                     DSGVRKLALMTAGKLNRTEFKQRITAIAQGDPEEHLRKAASVAAKKLEVR"
     gene            complement(12920..14236)
                     /locus_tag="PK224_10990"
     CDS             complement(12920..14236)
                     /locus_tag="PK224_10990"
                     /inference="COORDINATES: protein motif:HMM:NF019913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16307.1"
                     /translation="MSDIDVKYAALGGPAGWLGPATTAELVAPDGIGHYRHYRSGSIY
                     WSPASGAHEVHGLIRDRWAKLGWERSFLGYPLTDETTTPDRIGRYNHFQGGSIYWTPA
                     TGAHEVHGAIRGRWASLGWERSFLGYPLTDETTTPDRIGRYNHFQGGSVYWTPTTGAH
                     EVHGAIRGKWASLGWERSFLGYPLTDETTTPDGVGRYNHFQGGSVYWTPATSAHEVHG
                     AIRALWASMGWERSFLGYPTSDELSTEDSTGRYSEFQHGSIYWSPGTGALACRETVRL
                     HVKCLTAPTRFTINQMISNMRLTYATAQVGLKYVSFEVLNLPALNDIDVGACTMGTVT
                     AEQTQLFANRNNAAAKDVIAYFVRSTQPPFNGCASHPANRPGAVVASGASAWTLAHEI
                     GHVLGLSHVSDNNRLMTGLGTDNITNPPPDIIASEKTTMLASSFTT"
     gene            complement(14386..14562)
                     /locus_tag="PK224_10995"
     CDS             complement(14386..14562)
                     /locus_tag="PK224_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16308.1"
                     /translation="MNLFRYKPQQKKVMIRAAALLNNWYGAEIVQFEKLSCACSTWAF
                     RLLYDKLRFQRASA"
     gene            complement(14848..16503)
                     /locus_tag="PK224_11000"
     CDS             complement(14848..16503)
                     /locus_tag="PK224_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB16309.1"
                     /translation="MRSLTLLDERGAMPSLQALSEQDATRPVVLIGFQKQGNLGIGYL
                     AATLMRRGHRVKVLDFEDAAEQILATIEAEAPVVVGFSLIFQFYLPRFRALMQYLRNH
                     GVRCHFTIGGHFPSLSHERTLELIPEVDSVVRFEGEVTLLELVESLIHDQDWRRIDGL
                     AYRRDGRFVSNPLRHLLDDLDVLPFPYRQYEPTAILGQRTMPILASRGCSRTCSFCSI
                     HMFYRAAPGRVVRIRKVTEVVREMKDLYQNRGITLFLFQDDDFPLFGPSWRRWALSLV
                     DELYRQDLVGKVIWKISCRADAVEPELFATLRDAGLYLVYMGLESGSDDGLDVLHKEI
                     TVAENLQAVETLKSLGLMWEFGFMLFDPSSTFESIETNVGFLRRIVDDGSVAAVFCKM
                     LPYDGTPIKETLMAQGRFNGDVCNPDYDFLDPRIARCYEAIGRTVGEWVRGSDCVAAM
                     INHAWHEVFVMERLFEPAADLVAYKTALSAVTKFSNNILFSVVEEICDGFREGGGECQ
                     QYSGLGQIREQILATLVQQRNRFVYENQSWLLKGISKQTLVTA"
     gene            complement(16662..16895)
                     /locus_tag="PK224_11005"
     CDS             complement(16662..16895)
                     /locus_tag="PK224_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16310.1"
                     /translation="MEKPIMNPELPLPEVFKWRVPINWDPVPWWFFERANLPKEALSQ
                     LALIQLENQRAIMQQNMKAIEQSMEMVKKFGGK"
     gene            complement(17584..18138)
                     /locus_tag="PK224_11010"
     CDS             complement(17584..18138)
                     /locus_tag="PK224_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRB16311.1"
                     /translation="MTTQYYTASSLDGFIATLDDSLDWLFPLGDINETGYPSFIKEVG
                     ALAMGSATYEWMLRHVIRPDGREPVAWPYAQPTWVFSRRRLPSVEGTDIRFVSGDVLP
                     VHADMKKAARGRNVWLVGGGELVGQFYDAGLLDELIVQVGSVTLGSGKPLLPRRITSP
                     SLILESVRAIGPGFAELRYRVPRG"
     gene            complement(18248..19021)
                     /locus_tag="PK224_11015"
     CDS             complement(18248..19021)
                     /locus_tag="PK224_11015"
                     /inference="COORDINATES: protein motif:HMM:NF018755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HRB16312.1"
                     /translation="MPDIVHAQFTHTNAYLTQLIEQFARIDQQIENGDTITINMSDGD
                     RSMSLTVSRDKVVERATPPQRSATGKAPALADNDSLVAALTKSFSFDIGEGNRKISTR
                     EIQSHISNATAAKPAVGSGANLRRALELPEPAVTYNNAEWVLLDDCRCAALDGFMVTA
                     KRGFKTDLASIPRLLWGFIASFELSLVAPIVHDLIYGSAGTVALPDGEVTPADKRFDR
                     KEADDLFLELMTRSKVAYWKRNVAYLAVRAFGGSSWRQG"
     gene            complement(19135..19731)
                     /locus_tag="PK224_11020"
     CDS             complement(19135..19731)
                     /locus_tag="PK224_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB16313.1"
                     /translation="MKGTGFFTGLVVGIVATVLLSQWTMSAASKEAGTAPCLPAEMVA
                     DYVYSVIQADREFYTTDIVERMQLRGIVFAAENWRETSRLPLPAQFLLESGRLVAQQR
                     NGIRFRLISNWAINKTNRPATDFERAGLTAILINPDRPHTGVTTEGGRPVFQALYPDK
                     ALSQTCVSCHNAHPNSPKKDFKPHDVMGGILLTLPLPH"
     gene            19974..20408
                     /locus_tag="PK224_11025"
     CDS             19974..20408
                     /locus_tag="PK224_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HRB16314.1"
                     /translation="MTGKFDPESHEWARHEHVVVFDGVCNWCNAWVNFLIDHDPHETF
                     KFGTLQSDRGQQILHTLQLSTEDFSTFLLLEQARVYTKSTAALRIVRHLPGFWPLFYL
                     GILIPRPLRDALYDYVARHRYRWMGKTDACRVPSPDQRRRFV"
     gene            20553..21011
                     /locus_tag="PK224_11030"
     CDS             20553..21011
                     /locus_tag="PK224_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB16315.1"
                     /translation="MNHLFTASIFVAIAMALSVPSPAVAQNFPPSVTQKIHAAQKQVK
                     TIGLEEYRKVVESPGEALIIDVRQPQEFAAGHVPGAVNIPRGLVEFRIWKQVGFPANP
                     DTNRPVYLQCQNGNRASLAAQSLAELGFTNTTAVVMSFDEWQQAGHPLVK"
     gene            complement(21210..22526)
                     /locus_tag="PK224_11035"
     CDS             complement(21210..22526)
                     /locus_tag="PK224_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16316.1"
                     /translation="MRLASIIGLLTAGLLLCAPWSLAGTIRVDLGGEQGPMNHRANGY
                     LVSIEPTDPPMELILPLKPTSFRGNTGYVLSNYDRLKQAGVTEFQLTLGLVFEHRALQ
                     IFDINQIGLDGNYEPWLAHVDHVIDQVLQRQLSVIWDIYNEPDLAAVPLNDSERLKEG
                     WRVAYQRIKQRIPGAQIVGPSVSRYRLLKPFLEWSDSQGVFPDVVAYHEYADPSDAIR
                     YVNDLRDYLKAHGLARPISVNEILGQESWTSAGYTAGVIAAYERADILSAMRSCWPDP
                     QDMSRSYVENTCDNPTLDGLLYVDRKQKRPGWHVYKTYAEMEGVRLYTMTDSPKLHAL
                     AALDKAAGTLRLLLGKYEDDSPGDTQVVLKNIGRLFSSQHSGTVHLCAEQIPDVGSGP
                     LEAPVVTLDRALSVENEELSFTLPQFYHSDAYRVVLSLSEPCRASH"
     gene            complement(22625..23818)
                     /locus_tag="PK224_11040"
     CDS             complement(22625..23818)
                     /locus_tag="PK224_11040"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB16317.1"
                     /translation="MRRVIFVDDAPEILKHLQRTLAPMQAEWTMEFYPSATAALAAIR
                     ETPCDVVVSDMTMPGMDGAQFLGEVRKVCPQATRIVLSGDQSPANYLRSASVAHRFLQ
                     KPFDVATLKATIEKAEALRDVLANPALRSLANEIKTLPSLPSIYQELMKEMQAPQASL
                     KKASRIVAKDLGMVTKILQLVNSAFFGLRTHVSDPEQAVALLGFDTIKSLVLSSQVFA
                     QFDQAQLPSFSLDELWRHAMLAGTCARRIAKETGASQAVMDEAFTAALLHDVGVLVLV
                     ANKPDDYARVLELMQSKQMPDWEAEREVFGADHAHVGAYLLGIWGLGDGIVEAVAYHH
                     HPDDYKGSGFCAVTAVHVANAMAEAQARPGENPTEVPGLDQHYLAREQLMPQLPRWRE
                     GCIAA"
     gene            complement(23971..24489)
                     /gene="gspG"
                     /locus_tag="PK224_11045"
     CDS             complement(23971..24489)
                     /gene="gspG"
                     /locus_tag="PK224_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRB16318.1"
                     /translation="MGWRHYLNSSGSRRTGAVTDSWGTQLNRLRSCGGFSFIEVMIVV
                     VILAILATLLIPRVMGRTEDAKRAAAKAQISNIDSALQLYKLDNGNIPTTEQGLKALV
                     ERPASGPAAPNWKAGGYLPKVPVDPWGGPYKYTTPSTQGGEFEIISFGADGVPGGEGK
                     NADIVSWDLDRN"
     gene            complement(24814..26598)
                     /locus_tag="PK224_11050"
     CDS             complement(24814..26598)
                     /locus_tag="PK224_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16319.1"
                     /translation="MPPDSLVHEILRRPGTLTFPSNLTDLPHLTAVAGSVSQQPTGHL
                     VVYGSHGRRLCATDPDGHPLHECEWGPVDGALRLLRARVHLDWGAWVGLTPSGLVNTM
                     TLDLSRKPGWQRLRADDLRGMAAQAMRVPLEEVRFFYNDQDVTIGATGAATIRHRKDA
                     IYFLPDGTFDSKQFMACMGAMHWEEIDFLPVVELFLSLLPGTGSALFELIRGLYDDQN
                     RTKPAPRALRYRGIPTYPSEAAYRLFSNFFSPQIPGGGDPFPLFMDPPRSHMVTWLPV
                     SAPPRRHVDATQKLCVTIQGQRILKATCWDDPAGLSYQPFDRCGAAPCERGVAVEQGQ
                     LLLVDRGTRRVLPLGHSWGTVTGPPMVGAARGGIDWTAVFGGAAPVVSPDEAFGAVLL
                     YPDDEREIGELPTQPFVADYLQDLIEQDRPLAAQVGRAGHQLISGFDAAMTTCIGGDR
                     PRAYTVLYDHPAFAQRQAQMLWNLWARAQRLDWLSHVRLSARVDERGDLNNRTYDLAY
                     EWTPFNHYDKPDAVAARMQQLARQLLPGAVVFIVGPSAVADGCRAAGMHVQAITPVAS
                     LPTFRMHQSVLPRAQLKPGVMLFQIARP"
     gene            complement(26599..27912)
                     /locus_tag="PK224_11055"
     CDS             complement(26599..27912)
                     /locus_tag="PK224_11055"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRB16320.1"
                     /translation="MEQDLTESPVPELDAPVEVLTPEEYVATLVKRAKGAAGRLSSLP
                     TAVKNQALEAMADGLEEHETELLAENEKDLEQFEATPERKALGDRLRLTAERIREMAA
                     GIRDIARLPDPLGDTPKMWTRPNGMQVGRMRVPIGVIGIIYEARPNVTADSAALCLKS
                     GNVCILRGGSEALHSNVAIAKVLSESAEKAGVPAGAITFVDRAEREVVQLLLKQDRSI
                     DLIIPRGGESLMKTIAEHATIPVLKHDKGVCHTYVDVDADPQAAERICLNAKVQRPST
                     CNAMETLLVHHSIARTWLPLLVEKLKAAKVDVRGCAKTCQLCPEVTPAAEDDFGREFL
                     DLILAIKVVKNMEEALEHIATYGSQHTEAIVTKDYARAMRFLREVDAGAVLVNASTRL
                     NDGYQFGLGAEIGISTSRLHARGPMGLEELTCSKFVVLGSGQIRE"
     gene            complement(28037..28621)
                     /locus_tag="PK224_11060"
     CDS             complement(28037..28621)
                     /locus_tag="PK224_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRB16321.1"
                     /translation="MRGHLRNTDGITYLALMFSIVLIGISVSAAARQWTVMVQREKEA
                     DLMAKGIEIQNAIALYSNTMKIGRILPSEIYPQSLAELTRTPKPYLRRVYQDPMSGGD
                     WEYMRAPTGGIMGVRSKSKAKPIKERDFPLAVRHFEGRKTYHDWMFQHPNPSSMSMLM
                     PPMMGLAPGTMPMQPGPQGSAPGAPPTPGPARNP"
     gene            complement(28635..29336)
                     /locus_tag="PK224_11065"
     CDS             complement(28635..29336)
                     /locus_tag="PK224_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16322.1"
                     /translation="MNRVSKNQGLLLMTLVVLWIGLLVWQFGHFSEQERVPLTHVSGQ
                     RTVGKAPAAAVAAGGNLQVHLERLAAIKGQREATFATPRNIFASLLPQEPVVVPPPAS
                     RRGRARGPAKPPSPAGEPVVEAPQNEEEEPTVEEPKGPTPEEIERLRIATELNQFRYV
                     GFMAVGGGSQKKKNMAVVVKNDEMHVVQVGEVIAGEVLVKAVSMSEITLQDVASKITQ
                     VIPVTDAPGAEGQPN"
     gene            complement(29342..29914)
                     /gene="pilO"
                     /locus_tag="PK224_11070"
     CDS             complement(29342..29914)
                     /gene="pilO"
                     /locus_tag="PK224_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRB16323.1"
                     /translation="MMFPGLDRLQLTWRKPYAPILPWVAFVAGLCVLSYGIHALVLGA
                     AEQEQVRLEADWLKARQQLNRHKEAKKAKADLQRVWAVLPLFRDFAPLALGVTEEAKR
                     DQVTLPALSYKTEKTAVPDATKAVLQGTVTGRYEDLRRFLHNLESAEELLFIEDLNLI
                     RSGNVQDQQLTFNIRISTYLRGEHLQSPTP"
     gene            complement(29911..30537)
                     /locus_tag="PK224_11075"
     CDS             complement(29911..30537)
                     /locus_tag="PK224_11075"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRB16324.1"
                     /translation="MSLDNLVSAIRDVAVRVGQPAARHGAFAINMSSRYRWYLAPARL
                     VIMLLAGAIGLAMLWDISQAWLVWQDVESMESALNQVQDRDRDLLKEAQQEGLDLSEA
                     ALQVLPKEIEFANHLIEKRSFSWTRFLTELERAIPQRIAVNSIRLDPANATIMLTGSA
                     RALEDVTTLTVTFQDHPQFKEPVLGQHRVMASGLVEFDLSLRYRNRNP"
     gene            complement(30541..31548)
                     /locus_tag="PK224_11080"
     CDS             complement(30541..31548)
                     /locus_tag="PK224_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16325.1"
                     /translation="MWDWITSRPQYCLKIGARDLAWAEVHRNWRGRPRYQCVVSALPE
                     GIVRLSPLEQNIISPHELETHIRAIAGSATTQAPGRSSTPGVPRAATIILPDLAVRLA
                     VLRLQDLPWSSDERDALVRWRLGQEQLLSLSGAKVFSQVLSDPGGSGDGPCTVLAVVV
                     QEAVLTQYEAVCEAAGLIPQEVDVASLRLFNLWAHGTGRAARSTADFLWVNATDGGVT
                     ALIFHNGTLVFLRSKLQGGIGSGGALAPGSEQVALNRIVQECADSIYACQQQTPELAI
                     SQAVLIADEAMGAGLRQQLEKGLGVPVQELEWDRVQQYGGGTIAGPRTSAALPAIAGV
                     V"
     gene            complement(31552..33285)
                     /locus_tag="PK224_11085"
     CDS             complement(31552..33285)
                     /locus_tag="PK224_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRB16326.1"
                     /translation="MLMQRHLTRPSLADVMVREGILPRRTVDQVVARLGGSTTAFGQT
                     LVEEGTISEAQLAQALAAQYGLPYDPLTGFRVDSEFYHTISVKLMRRHPFVPVKDEAG
                     ALTIAISDPQNLLALDELEILLNRPLHYVVSSRSAIQSALERSEGSSQALRELEAEYR
                     SVLVKEDERGEEVLTVDHFGEDQSPVVKLLDTIMLSAMQRRASDIHIEATDRATTVKF
                     RVDGILVSAMDPLDVKLHPPLVSRLKVMSDLDIAERRVPQDGSFRMRLDRKTVDFRVS
                     ILPSVFGESVVIRILDREAITTGVSTLRLDRLGFNPEDLKRFRRAITRPYGMVLVTGP
                     TGSGKTTTLYAAISEMNIQEDKLITIEDPVEYQLPGVVQIPVNEKKGLTFARGLRSIL
                     RHDPDKIMVGEIRDAETAQIAIQSALTGHLVLTTVHANNVFDVIGRFASMGIDSYNFL
                     AALTCVLAQRLIRVICPDCRHQVVLDQALAEESGLDYDEFKSAPFYEGKGCSECHDTG
                     YRGRKCITEFLDLTDEIKEMILADRALSEIRYRAVTDGMITLRQSAVKKVLAGETTLR
                     EINRVTFSEER"
     gene            complement(33353..34567)
                     /locus_tag="PK224_11090"
     CDS             complement(33353..34567)
                     /locus_tag="PK224_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRB16327.1"
                     /translation="MAVFAYRAARADGTTFEGHIEGEDEHLVRAKLESDGLLVFRLSR
                     RGAGFGVPGLAAGRWGKLPLQDFLVFNQELLALIKAGLPVLRVWDLLIDRTQRAPFRE
                     ALKAVRQDIRGGASASEALGKHSTYFSDLYLATIRAGEQSGNLAEVLQRYIAYLKLMI
                     GLRQKVTKALAYPAFLVVVGVAVVGFLLSYVMPTFISVYGESSANLPTATRMLISVIQ
                     MGEAQLIPVAIVAVVAVVMGRAWYQTSAGRFSVDRNLLKLPLLGMILVEHHTIQLTRT
                     LATVLAGGTPLVEALEIARGAVSNRFVSRGLVSAVNEIREGSTLAAAIERPQILPKLA
                     IEMLSVGEETGSLEPMLRDVAEFYEGDLDVRLSQLTTWIEPVLLLVMGLLVGGIVIIM
                     YLPIFQMAGTIQ"
     gene            complement(34608..35438)
                     /locus_tag="PK224_11095"
     CDS             complement(34608..35438)
                     /locus_tag="PK224_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRB16328.1"
                     /translation="MIQQAGPVWRTVVGLRFGCSIVATMSLLFWGGCARVTPSPESTP
                     TDLQVAADSLKTAVREAQRTAAELRTELEEQRKELADAQVARAQLQGMLRETERRLAD
                     ARQVIELQREELTSAHTERERVAQTVAPLHSRLQQPPNGVRLQRKRASSSPDGAVPAP
                     RDTQEQSAVKPLENEEEEAPQSAPADGEAAPVSDVPQASPIVMPIPSAAEPVRTIVVQ
                     GGDTLWRLARRHKVSLEALKSLNGLPTNLIVVGRTLRLPEPRLQQAAMQPVSLKTLVR
                     "
     gene            complement(35452..35859)
                     /locus_tag="PK224_11100"
     CDS             complement(35452..35859)
                     /locus_tag="PK224_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB16329.1"
                     /translation="MARNTEIGKRSWPFSSPHGFTIIELMIVVTIVGILATLAVPSYH
                     AAIIKAKEGALRQDLFSLRDVIDQHRADKGKYPETIEALVSAGYLRRVPTDPLTGSTT
                     TWQEMVDEGEGGMVDVFSGSDLVGTNGVPYNQW"
     gene            complement(35834..36340)
                     /locus_tag="PK224_11105"
     CDS             complement(35834..36340)
                     /locus_tag="PK224_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB16330.1"
                     /translation="MKQSGVTLLELLVTLTILTILATVALPFTKVSTKRTKEIELRQN
                     LRVIRAAIDTFHLEWSRDGDTLTGPACVKNKLSCKDVTGPYGYPKSLDVLLGVKLTGE
                     QATVRGTTIKRYLRSIPLDPVTGAADWRLRCYRDPASVKDWCGEDVYDVTTHSQELAL
                     DGTKYRDW"
     gene            complement(36337..38715)
                     /locus_tag="PK224_11110"
     CDS             complement(36337..38715)
                     /locus_tag="PK224_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRB16331.1"
                     /translation="MRSSFRTTHVETRDHWCCRVLISTAGVVCGLLITGCGTFVSPDV
                     KRGDQHLAAGNWEEASVAYRQALKDDPFEPSLQNKYSVARERAAAAHDERGRQLLKDH
                     QLDQAAEEFKRALTIEPTSKEHEAGLTEALRLKEARERYREAERLAQLGRVSEAMAVY
                     MRAVELDPTYKEALEGVARLSAEQHAVDRDDRQKQPVTLQFRNAGLKEVVEALGKAAH
                     VNFVFDKDVRNDPVTISLEDKPFDEALSLVLNSNSLFAQKAGPTLFIISPNTKQKQEQ
                     YQDLMIRTFYLSSAKAKEMVALLKSMLDVKHIHGNEALNTIVIRDQPEKVELAEKIIQ
                     ANDREDSEVLFDVEVLEVNRTVDQTYGLSYPKQIGAALIPPGFTGAIAGDIAQQFTYR
                     NLASLGQDSYLFKLPTNVQLDFFKQVTDAKTLAAPKVRVLNNKKAEVNIGDKQPILLS
                     TTNVLPGQAATGAVPTTSTVTSIEFRDTGVKLTVEPNIHLANELSLKMKIEVIRLGDV
                     VLLQQSPPITQFKFGNRSAETMLNVRDGETIVLGGLLQEEDRRTKVTIPWLGDIPFLG
                     NLLSSFKTQRVTTEVILTITPHIVNSLRLPGPQGQAFWSGTESVYSTSPLFAMQPKKI
                     SARMSTLSGSGKLAEKGVSKQSKGTVASADFVPLELQLAIQPADVTAQMNKELRVDVL
                     ATQARGLDTETFTLEFDPKILEFRDATLGEVLGTEAGKAAVTATPSATEGLIELRLHR
                     SASATKDEGRLLRLTFLAKSPGVSSLRLQMAKHDDLDSPEGTGEAVGVVRVR"
     gene            complement(39001..40296)
                     /locus_tag="PK224_11115"
     CDS             complement(39001..40296)
                     /locus_tag="PK224_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRB16332.1"
                     /translation="MAKKMQRQIAVVGLGYVGLPIAVAFGKSAPVIGFDINKAKVDEL
                     RKGIDRTGEVSSKDLKASRVRYTSEPSDLKTADFIIVAVPTPINEALQPDLTALKKAS
                     ELIGSNLAPGAIVVYESTVYPGATEEDCLPILEKASGMKSGIDFKIGYSPERINPGDK
                     EHTLERIIKVVSAQDEESLEIVAKTYGMVVKAGIHRASSIKVAEAAKVIENTQRDLNI
                     ALMNELALIFHRLGIDTRSVLEAAGTKWNFLKFSPGLVGGHCIGVDPYYLTAKAESVG
                     YHPEVILAGRRINNSMGKYVAEQTVKLLSQVERPVSDLRVGVLGLTFKENVPDLRNSR
                     VPDIVNELKEYGIQVVVHDPLAEPEEAVGEYGLRLSPWDQLKQLDGIVLAVAHREYMQ
                     MSVSELLKPLRKQRNNVVVDVKSVLNPDSLPGSVKYWRL"
     gene            40876..41772
                     /locus_tag="PK224_11120"
     CDS             40876..41772
                     /locus_tag="PK224_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16333.1"
                     /translation="MDGNSTSQASKRLATIVAVLTFCAGSVSAQHSHAAGVWTNEPAG
                     ANVVVDCNFSSTPGACGILDAYSSAEVASDSSAPLSPSGVLKSTLRAGNSAGGMQLNF
                     ATPKPNNREMFMGIIWRTNPEFQGRPNSNKLFFLRGPQANGVWIFGNSALVNGSAPLI
                     FGHNTGGGLLDNSHACSADLGLLCFPNVGPGLLTRGVWTKIEAYVKSSTTNTSRDGIV
                     RWWINGVLVGNYTNLNYGGTAGINEWVWSETWDGGVNPLPTVDWNHFIDHLHISIPNG
                     GSGSDIPPGPPAQPTLRSITVP"
     gene            41788..41859
                     /locus_tag="PK224_11125"
                     /pseudo
     CDS             41788..41859
                     /locus_tag="PK224_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(42051..42545)
                     /locus_tag="PK224_11130"
     CDS             complement(42051..42545)
                     /locus_tag="PK224_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HRB16334.1"
                     /translation="MDFVHDTLSEGRPYRILTVVDNWSRSSPVLEAGFRMSGALVSQV
                     LDRVLGEGQRPRSITVDHGTEFQSRALEDWASRRGVQLDFIRPGKPVENAFIDSFNGR
                     LRDECLNVHQFASLAEAQAIIEAWRVDDNTRRPHSSLGHLTPSEFVRQRQEEQDTENV
                     VCSP"
     gene            42775..43044
                     /locus_tag="PK224_11135"
     CDS             42775..43044
                     /locus_tag="PK224_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB16335.1"
                     /translation="MARRRGHTEEQILAALRQAESGPTVAEICRTVGISEQPFYVWKR
                     KYAGLALSELRELRQLREENTKLKRLVADLSLDRHMLQEIVRKKL"
     gene            43128..43691
                     /locus_tag="PK224_11140"
     CDS             43128..43691
                     /locus_tag="PK224_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HRB16336.1"
                     /translation="MARASLRYQGHRDPQEALRMRMRELAAARVRFGERRLTVLLRRE
                     GWSVNAKRIYRLYSEKGLTVRTKHRTKAAGRARVPQPGATAPNQRWSMDFMSERVADG
                     RSFRILTVVDQFTRECLCLLADRSLTGEKVAQALELLVGQRGVPRSITVDNGSEFASR
                     VMDAWAYRHGCNGLSSSGPRMCAWRGR"
     gene            complement(43650..44618)
                     /locus_tag="PK224_11145"
     CDS             complement(43650..44618)
                     /locus_tag="PK224_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HRB16337.1"
                     /translation="MRLITTKELRTLHIATRHHHTINSRLAVLRYAQDYGFKGAARRF
                     GLDRKTVRTWHRRWAASGPAGLVPRHPVKRRRRLSEETVQLIEQARRDLQFGAMRTRI
                     WLNRVHQIRVASATIRRICRDLGYPPIRRTDPRRPRHPILFSKEQPGECVQVDVKEVK
                     VAGQKCFQYTALDDCTRYRVLRLYPRKYHGTSLEFLATIRQVFPFSIRKVQVDNGTEF
                     PLAFALAVQAAGIRLRHIKPRRPEQNGKVERSHRIDEEEFWSRTTFDGFASATQALRT
                     WEHQYNHDRFSMALQGLTPAEKLATFLSPPAPSSPTTPCTHTGAAA"
     gene            44617..45867
                     /gene="istA"
                     /locus_tag="PK224_11150"
     CDS             44617..45867
                     /gene="istA"
                     /locus_tag="PK224_11150"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRB16338.1"
                     /translation="MGSPLVIACDCNPCRGPTLNKIFHTLQRRIRRWRATHGPEREVM
                     FPQIHRPGEYAQSDFTAMGALGITLNGQPFDHLFYHFVLPYSNWETGALCVSESFEAL
                     IAGFQAAVWELGRVPALHRTDNLSAATHDLREGGRTFNERYRQVLDHYGVRPDANTPG
                     RGHENGDVEQAHHRFKRAVEQALLLRGQRDFDSQAHYAQFLRAILAGRNRGRQEALQD
                     ELPHMRPLPALRLDDFRVLRVMVNRFSMIRVGHNVYSVASRLIGETVEVRVYPERLEV
                     RYAGEGVATIDRLRGRGQAAIDYRHLIGSLVRKPGAFARYRYQATLFPTTTFRRAYDA
                     LTACHGSRADVEYVRLLQLAARTSECEVETVLGRLLDGGTRWDAMQVKALVQPDVIAV
                     PPCALPPPNLSVYDACLDVVGGGR"
     gene            45864..46691
                     /gene="istB"
                     /locus_tag="PK224_11155"
     CDS             45864..46691
                     /gene="istB"
                     /locus_tag="PK224_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRB16339.1"
                     /translation="MTTLPVLLRALCLPTFAREYAVVAAHAAREGLSHEAYLQTLATQ
                     EASDRAARRVERLIVEAKLPRGKSLATFDLARLPPKVRTAVATLREGAFLDQATNVLV
                     FVNPGTGKTHLVCGLGLELVRQRRPVLFAPTFQLVQRLLAAKRDLRLAAELKRLDRFD
                     ALILDDLGYVQQDREEMEVLFTLLAERYERRSVLITSNLVFSKWDQIFKDPMTTAAAV
                     DRVVHHAVILELPIPSYRAQAAKARSQASSGAATPDRKSMGDTTVNDERTDGARTAP"
     gene            46809..46961
                     /locus_tag="PK224_11160"
     CDS             46809..46961
                     /locus_tag="PK224_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16340.1"
                     /translation="MVDQERWAEIRRLFDEERVSISEIGRRLDVDRKTVRRSLRQPTW
                     CKLPIN"
     gene            47017..47831
                     /locus_tag="PK224_11165"
                     /pseudo
     CDS             47017..47831
                     /locus_tag="PK224_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611873.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         9826 a        14921 c        13502 g         9656 t
ORIGIN      
        1 gtcccgctcc tgtcgtgcca tgatgcaaaa cctcctctat agatacaagg tcttgtaatc
       61 acacattccc cggcgcaccg cgggccactc ctcccccttc cacctcattc ggtctcgttc
      121 cccgatcgac tcactcggcc gctcggccta tggtttcccc tgctcgcccg cctcatctcc
      181 cggtccatcc gctgcttgaa caacttgtcg gcgcatggtc ccgtcccatt cgatctgctt
      241 gcgactgtcc ggctgccttg tagaacggca acgtagcccg gcacgagttc tgaaattgga
      301 gcatcgtcct ttcttgaaag aaaagaaatt taatgaggcg cccataccgt gtataacgtt
      361 ttcccgagga cagggaacat cggcttcatt cacatcatac ccggaggaac ccggcatgcc
      421 tgagaacaat cacagcaacg ctccgctgcc acccccgcac gtccaattga tacagatggc
      481 catggcccac tgggtctccc gcattgccta cgtcgccgcc aaactgggat tggccgacca
      541 cctcgcgagc gggccgaaga ctgccgacga cctcgccggg cccacgggaa cccatgcccc
      601 ttcgctttat cgcctgatgc gggcactcac gaacctcaat atcctcaccg aggacgccac
      661 gcaccgcttt gccttaacgc cgctcgggga ggcggtcaag accggcgcgc cgggatcggc
      721 acggccttcg atcctgacac tggccagcga ctggatgtcg cgaggctggg agcaattgct
      781 ctattcggtg cagaccgggg gctgtggatt tgagcaatcg ctggggatgc ccctgttcga
      841 ctggctggcc aagcatcccg aggatgcgtc gttgttcagc gaaacgatga tggggtttca
      901 tggcgcggag ccggcagcca tcaccgccgc ctacgatttt tccggcttat cgaccctgat
      961 cgacgtgggc ggggccacgg gacatctcct gacgacgatt ctcaccggca atcccgcatc
     1021 acgcggcatc ctctacgacc tgcctcatgt ggttcgcgac gcaccggcgc tgattcaggc
     1081 caggggcctg gctgaccgcg tgacgatcga ggccggtagt ttttttgatc gtgtgccggg
     1141 cgggggcgat gcctatctgc tgtcgcacat catccacgat tggaccgagt cgcaatgcct
     1201 caccattctc ggccactgcc gccgcgcgat gacacccggc agccgcttgt tgatcatcga
     1261 gatggtgctg ccgtccgaca acgctccgca tccgggtaag atgctcgata tgatgatgct
     1321 cgtgggtcca ggcggacagg aacggactga atcagagtac cgtgagcttc tcgggaaagc
     1381 gggattccgt ctcacccggc tcgtgccgac ggaaacggcc gtgagcgtcg tcgaagcggt
     1441 gctcgcataa agtcacatct gatgtgagca aagagtacgg cgacagacag ggctcccgcc
     1501 gtactcctgg cgctttctcg actcaggacg tccccgccgg ctcagtcgat gatgcgactg
     1561 gcccgcaggt cgcccggctg attgggcagc agcatgatgc agttcgggtt gaacccctgc
     1621 gccttcagcg tgtcgatgtt ccccaaaacc ttatccgcct tcaagtcgat cactgtgatg
     1681 gacccatcgt tcatgccgtc cagattgagg aagctgttct ggacgaacag gtagcgttcg
     1741 tccggcgaca ggacgctgtg gtgcgcaccg cccgcggcgg cgatcgtctt gagaaacttc
     1801 ggttgccggg gatcgctgtt gtcgtacaga ttgacgaaac ccggtttggc cgtcgtgacg
     1861 aacaggcggt cccccttggc gttgtagagc atttcgagcg gcatgccctg ctgccgggag
     1921 ccaaagtcat cgacctgatg gaacgaaaag gttctgcttg ccgggtccca caccccggcc
     1981 cacaacgttc cttcgagcat attggtgata tgcacgacag gaggattcgc attgggactg
     2041 aacatcactt ccacgggagc ggcttttgcc ggagatggtt tcgacgagac cttgtgcgtt
     2101 gagagcacct tgccgctggc ggcctccagc accgtgatcg aatcacccgc atcggacata
     2161 tccggcttga cggtactggt cacgagcagg cgatcgatgc cgttgtgaat cgcaatgccg
     2221 tgcggatact gaatcgtcgc cagggacggc ttcgcggcgc tgaccgtttt gatcggctta
     2281 tcgaggagcg cgtcccccat gatgacgttg tccgagccca tgcaggtcag gtaccaggtc
     2341 cggttatctt ccgacacggc gagatcctcc cccatctgac agtccggcac gtcgatcgcc
     2401 tgaagccgat agggaaacgt cctgagattc acgacatgca gaatgctttt ccccagcgcc
     2461 gtgatatagg ctttgctccg atcgcgattg aagaaaatat ggtgcgccac gagatcgggc
     2521 ggcagtggaa tctccatcag aatcttcccg aagttcccgg actcgggatc gatgtccatg
     2581 atggcaatcc cctcccgtct caccggctgg tccggcttac tctcatagtt gagcagggcc
     2641 aggatttccg ccgatgcgag ggtggagcac gaaagcaaca accccagggt gaacgcgaac
     2701 aatctatatg gtttcatggt ggcctccttt tcgattgtcg aaattggact cactttggct
     2761 gagtacgttg tccaggcgta tgcacggccg gctctctcgg cccgccgccc ccctcacatt
     2821 cacttcatcg catgggtgcc atgcgtgatg gccccgcctg cgcgcaaggc cgctgccacg
     2881 agcaccgatt cggcgatctc cgggtccgac gcgccatact ctcttgcctt gttcgtatgc
     2941 agttcgatgc agtagggaca ttgcgtggtg aacgccacgg ccatggccat caattccttg
     3001 tacttcaccg gaatcgcgcc ggctgccagc gcggccttgt cgaaggccac gaatgccttc
     3061 atggcctcgg gggcgtgcgt ccacagatcc ttcatctttt ccagattcgt catgtcatac
     3121 aggcccgtcg tctgatccat ctcggttgtg gtcgcgttcg tcatgatgca tctcctttgt
     3181 atgggcaatc gaggctcacg cccgaatgcc gataacttct aaatattcgc cgtacaccgt
     3241 cgtgcagtcc ggccccgctt gattgtgcgc tgtccagagg gcttccaaat cccggcgcaa
     3301 gtgcgcttgc ccctcggcgt cgagggacgc aaaggcccgg ttggtcggtc catagtagag
     3361 acggaagaat tccaccacct ctgatggagg aaatggatag ctgaacagat actgacgcct
     3421 gatcaggctg agcgtggaca atccgtgacc aaggcgttct tgaaccgtag cttcattccc
     3481 ccagagcacc ggcgacggca tacctgacgg cccgatgaat ttcgcgacgt tcttgaacat
     3541 ctgtccgacg aacccctccg ccgtccagtt ggccatcgcg agggttcccc ctggtgtaca
     3601 cacccgcaac aattcctgcg ccaccagatc aggacgcgga gcaaacatcg cgccgatcag
     3661 actcaccacg acgtcgaaac tggccgcctc aaacggcagc gcttcggcat cggcgacttc
     3721 gaatcgggca gtcacccctt cggcccgagc ccgcgcttgc gcccgctcga cccaattggc
     3781 ggcgatgtcc acccccgtca cctccacccc atccttggcg gccaacaacg cgagttggcc
     3841 cgagccgcat cccacatcca gcaaccggca tcccggcgcg atctggagac gttcgtaaaa
     3901 ctccctggcg ccgccctcca tatatcgtga aaatcgatcg tagtcccctg ccgtccaaat
     3961 ggtattgaga cgggtcttca gaccgtccat ttccggcacc accgtgttcg tcgtcatggc
     4021 ctcctccttc ccgtgtgtgt tctctgtcct ggcttccgtc gctgcgtctt gtctggtcgc
     4081 actataggac agaggccgtt tcgagtctta tcagggcgtc tggctctctt atcaggagca
     4141 tgctaagctg cagttcgttt atccgggcgt ccggatggct ttgctcaggc tccggccttg
     4201 ccgaggaaat tcctatggac gtgctgtccg aagtgctcaa ggccgtgaaa ctcgacggcg
     4261 cggtgttctt tcacggtgag tttgccgcgc cctggtgcgc tcgagagccg gatgccggga
     4321 ccatggcgtc gtatctgcct acccaagccg gccacatcat tattttccat ctgctgctcg
     4381 aaggacgggg gtatgtccgc ctggagcagg aagaccgtgc cgtgccgctg acggcgggcg
     4441 acatcatcgt tctcacgcac ggggatgcgc actttatggg taacggcccg cccgtcacac
     4501 cggtggacac ctcccagcaa atccggcaga tcctctcgga gggacgcatg ctgtcccaac
     4561 tcgccggagg cggtgaagtc acgaaactga tctgcgggta cttgacctgc gacgcgcagc
     4621 tctgccgggt cgtcctggcg gggctgcctg cgatgctcaa ggtcaatatc cgggataccc
     4681 cgtcgggaca gtggctggaa aatacgctcc gctattcggt cgatcacgca gaggcctcgg
     4741 gccccggagg cgcggcggtc atcgccaagt tatccgaagc gttgttcgta gaaacgttgc
     4801 gacgctacat tgcacaattg ccgcacactc agaccggatg gctggccggt gttcgcgatc
     4861 cggacgtggg caaggcgctg gcgctcctgc atcaacagcc cgctcgcccc tggacgatcg
     4921 ccgcactcgc cgctgaggtc ggggtctccc gctccgtact cgccgaacgg ttccagcact
     4981 tcctggccga cacaccgatc ggctacctca cccgctggcg cttgcacctc gccgcgcaac
     5041 tcctcacctc cacgtccaag agcgtggccg aagtcgcagg cgatatcggg tatgagtccg
     5101 aaccctcctt caaccgtgca ttcaaacgcg agttcgggct tccgcctgcc cgcttccgca
     5161 ctcaggccaa atcgcccaaa cagccatcag tcggtcgtga gtccacaccc gccgagttga
     5221 agcccatcca gacgacatga ccggtaatcg ccggccttcg tccgacgcac acccggccgg
     5281 ctccgttccc actgaaacat tgtgcgacag ttctgagttc gatcgcggat gcaggccgga
     5341 tggcgcgccc gtgagttaag cgcgagatag atcgacgtgt taggcctgga tgtgactgct
     5401 caactaattg gagaagctcg ggaggcaggg ggcagtgact ttagcgtcat ttcttgtgag
     5461 aacatcgctg ccggacgatt gaaggcctcc gtggatcagg ctgtctgcaa cttcgcactg
     5521 ctggggaaag agtctgtcgc taacctgttc ctcgcaattc cctcactgct gaacggccga
     5581 ggagcacgtg tcgtccaagc cctctatccg gtcaagccac ttctactcag gaatctccgc
     5641 ttcgacaagt ttcgcgagga ggatcaacga ttcctgccag cctagataac aagactcaac
     5701 ggggatcacc tcgggaatcc cttcctgcac caccgtcatc tcagtcccgc atgatacttt
     5761 cttcaacgtg atcgacacct gaatttctcc gggaaggttc ggatcgtcga acgtatccgt
     5821 atagcgcagc cgttcgtgtg gcaccagttc gaggtacttc ccgccgaatg aatgactctg
     5881 accggtggtg aagttggtaa acgacattct gtaggtgccg cccaccttgg catccagctg
     5941 atgcactttc ccggtgaatc cgttcggtgg aagccacttc gccatggcat ccgcgtccag
     6001 gaatgcgcgg tacaggcgtt ctggtgttgt ccgaaagacc cggtgcaggc ggacggtatt
     6061 cttggacatc tctcacactc ctttcctctg agcgcttccc cagcgctcag gcgagcccgc
     6121 acattagaca tatttttaag gatgccggat tcatgcccga gtgtctagcc cggattattc
     6181 atgaatgccg tcgcgaggcg tttgagaccg acgcccgccg gggcttctcg cacgtagggc
     6241 cgacgcaggc gtacgtgcag tccgtcgagg aggccgaacg agaacagccc cgccgggcga
     6301 gaggatcgga caccggagca ggtattcgtg aatagtccgg gccaggcgtc aggctcgtca
     6361 ttgacggtcc gcagaacgaa tggcagaagc ctgaccggtc tgctagagta gcgaacgcgc
     6421 gtcggcttca ctcgaccgac tgcttcagga tgatcacatg caccggcggt ggcactcact
     6481 gctcgggcta actgcccggc tgcatcaacc gattagacaa ggatggcacc atgcactgtt
     6541 cgaatcccct cggctcacgc agactggtct ccttctcgct ggcttgtgcg ctggtcctct
     6601 tcggcctgat cgggtccggc tgccagaccg cctattatga aacgatggaa aaactcgggt
     6661 atcacaagcg cgacctgatg gtgagcgacg taaaaaaggc ccgcgacgcc caacaggacg
     6721 cgaaagaaca gttcaaatcc gctctcgatc gcttcaccaa gaccttgaat atccagggcg
     6781 gcgaattaca ggacaagtac gatgcgctga acgaggaata tcaacagagc gaaaagagag
     6841 cgcaagccgt tcgggatcgc atcgcgtcgg tggagaacgt gtccgacgcg ttatttgatg
     6901 aatggaccgc tgaactgaag caatattcca acgccacgtt acgccagaaa agccaaaagc
     6961 agctcaccca gacgcgcagc cagtatgcgc aattgatcaa ggccatgaaa cgggcggaag
     7021 cgaaaatgga tccggtcctg gcgaagctaa aggaccatgt cctctttttg aaacacaact
     7081 tgaacgcgca ggccatcgcc tcgctcaaga gcgaattgac cacggtcgaa gggaacatcg
     7141 actcactgat caaggatttg aacgcgtcca ttcaggaagc ggattccttt attgcgtcca
     7201 tggagaagga taatgcgtga tcgtggcaga tggacgccct gcccttctca ccccggtcaa
     7261 catgactcta tccactgcat cattcgctga cgatgggatc ctgtccccgt cagcggatcg
     7321 ccgcgcagat ctgccgaaca tggccgcgca gccagcgatg cgcaagatcg gcatccaacc
     7381 gagggtgcca gagcaacgac accgtgaact ccggcgtgga gaccggaagc gaaaaactat
     7441 gcatgcccgc gcgcaggatt ccggtatgtc gttcgggaac ggtggcgatc aggtctgagg
     7501 cacgggcgag tgcgatcgcc gtcgcgaagc cgccgacgat cgtgacaatg tcccgttcca
     7561 gcccgagcct ctgcaacgcc tcctcaattg gccccttttc aagcccctgc cgcgagacgg
     7621 agatatgctt tcccccggca taacgggaag gcgtgatctt gcccttagac agcgggtgcc
     7681 ccgttcgcac gacgccaatg agacggtccc ggaacaatgc ttgtacacgc aactcctgag
     7741 cagtggtctt ctcgatcacg ccggtttcca gatcgacggt gccgtcacga aggggtgcgc
     7801 tgtctttatt ggctttctga aggaagtgca gccgcacgcc gggagcttcc tccccgacca
     7861 gcgcaatgag atccggcccg aagttttcta cgaatccctc gctggtccgc aatgtgaacg
     7921 ttcggacgag ctgtgtgaga ttgagcttct ccgcagggcg cagcaccgct tgtccctctt
     7981 ccacgacctg actgacccgt tcacgaagtt caaccgctcg tggtgtgggg acgagacctc
     8041 ggccggccct caccaggagt ggatcaccgg tcgtctcacg caatcgcgcc aacgcccggc
     8101 tcatggccga cggactcagg cgcagtcgtg tggcggcgcg agccacactg ccttccgcca
     8161 ataaaacatc cagggtgatc agcaaattga gatcaggtgt ggacatgggt cgaccatacc
     8221 atagcttgac cgtgatgtgg cgtctcacgc acgaatacag tgcagatgat gcgccttccg
     8281 ccatgtcgtt tggaaggtta tacttccgac agttcgattc gggatacgcg ggaacaagga
     8341 gtgcgccggt cacttaacag agaggtcacg gactatggag ccacaatttt ggcatgacag
     8401 gtggacaaaa aatgagatcg ggtttcacaa gagtgacgcc aacccgctgc tggtgaagta
     8461 tttcaccgag ctcgctctgc cgaaaggcag cctggttctt gtgcccctgt gcgggaagac
     8521 gctggatatt tcgtggctgc tgtcgaacgg ctaccgggtt gccggagccg aattgagctc
     8581 aaacgccatt gagcaactgt tcgcggagct cggggtgaaa ccgacgatca cggggaacgg
     8641 tgagatagat cgctaccatg cgaacgacat cgacatcttt gtcggcgata tcttcgatgt
     8701 gacgagaacg atgctgggtc cggtcgatgc gatctatgac agggctgcgc tggtcgccct
     8761 tccggagaac atgcggagtc gctacaccgc gcacctcaca gcgatcaccg atcacgcgcc
     8821 gcagttgctc gtttgttatg aatacgatca acgcgcggcg gaaggccccc ctttctcaat
     8881 cagccacgag gaagtccgtc gacattatgg agagcgatac cacttaacgc tcctcaccag
     8941 cgcagatgtg cccggtggac tcaagggaaa atgttcggca accgagcatg tgtggctgct
     9001 acgaagaggc tgaggatgat cacgcaattc atggcaggac gagatgaggc acaagcagga
     9061 acgacggatc tggcgccttc gattcggtgg atactcgccg gcctgtcgtt atccatgttg
     9121 ttgtcctcga tcgggacgag catcgccaat gtgagcttgc cgacgttggc acaggcgttc
     9181 gatgcctcgt ttcaacaggt ccagtgggtc gtcatcgcgt atctcgtgac catcacaacc
     9241 gtgatcgtca gcgtgggtcg gctcggcgac atgaccggtc gccgacggct cctcctggtc
     9301 ggtgtcttcc tgttcaccgt cgcctcggtc ttgtgcggtg tcgctcccac gctctggctg
     9361 atgattgccg ctcgcgcggc gcagggtctg ggagccgcca tcatgatggc cctcactatg
     9421 gcattcgtcg gcgagacggt tcccaagtca caggtcggca gcgccatggg actgcttgga
     9481 acgatgtctg cgatcggcac cgcactcggc ccatcgctcg gcggcatgct gatttccgaa
     9541 ttcagctggc gggcaatctt cctgatcaac ctcccgctag ggctcctgat ttttcttctc
     9601 gcgtatcgat tcctacccgt tgatcgtccg cgacagcaag ttgatcggct cgggttcgac
     9661 catgcgggta cgctgctgct tgcgctgacg cttgcagcct atacgctcgc catgacgatt
     9721 ggacgcggca gctttgggcc gcgcaacctg gcgctgctgg tggccaccct acttggagcc
     9781 agcctcttcc tgtttgccga gacaagggct gaatcacctc tgatccgatt gacgatgttc
     9841 cgcaatcctc tgctgagcgc aagccttatc atgagcgcac tcgtctcgac ggtgatgatg
     9901 gcgacgctgg tggtcgggcc gttttatctc tcgcgtacgc tcgggctcca cgcggcactt
     9961 atcggactgg tgttgtcggt gggccctctt tgcgccgcgc tgaccggtgt gccggccggt
    10021 cgccttgcgg accgccttgg tccccaacgc atgaccgtcc ttgggctcat cggaatagcc
    10081 gccggtgcct tcctcctgtc cgtaataccg gcgacgctcg ggatctatgg ctacaccgcc
    10141 cccctcattg tcatcaccgt cggttatgca ctgttccaga cagccaacaa cactgctgtc
    10201 atgacggata ctcgtccgga acagcggggc gtcatttccg gcctgctcaa cctgtcccgc
    10261 aatctcggac tgatcaccgg ggcttccgtc atgggcgctg tgttcgcggc tgcgtcatcg
    10321 acgatcgaca tcacaacggc gcctcctgct gccgttgcca tcggtatgca ggtcacgttc
    10381 gcagtcgcgg cggttctgat cctcgtcgcg ctcgccatcg ccgtggcaac gtaccgccgt
    10441 gagttgtacg ggtgggcggt ggctccgacc gcagagtcat aggcggacag cgggattccg
    10501 gcagcaccgc ttgccgctcg tcatccgcaa gtgcctgtgg cacaggttgc gaaccatgct
    10561 gcacgagaca tggattgcat ccctttgcgc cacgatggac tcgaccggcg agaccaagta
    10621 gaatgacccc gttcttctcg tcttccccgc gcgacatcgt catcatttcg tcgggtgcag
    10681 ctcagcctgc aaggatcgtc cattacccca agggagctac atgcaaaaga ctcgcatcat
    10741 ctgcacgatc ggtcccgcca cagaatccta cgaaatgctg cacaagctct acgaggccgg
    10801 gatgagtatc gcccgtctca acatgtccca cggcgaccac gaatcccacg ccaaggtcat
    10861 ccagcacatc aagtcgctca accggaaggt aaaatttccc attcccattc tcctggacac
    10921 gcaaggtccg gagatccgca ccggcgatct ttcgaacgag ctcgatctgc ggcaaggcga
    10981 cattgtctcc gtcaccaccc gcggtccgat gaacgtcgag gaaagctcca tccacatcaa
    11041 ctatgcggac ctgcttgagg cggtcaacgt gggggaccgg atcaccgtgg acaacgggct
    11101 gatcaatttc gaggtgttgg agaagcacga acgccacatg cggtgccgtg tcttagacgg
    11161 cgggcttctg aagagcaaac gccacgtgaa tctccccggc gtccgtgtca atctcccgtc
    11221 gatcacgcaa aaagatatca aggatattct ttttggtctg gagcgggatg tggacttcat
    11281 cgccctgtcg ttcgtgcgcg aggccggaga tattcagcag ctgaaggatt tgatgggcga
    11341 caaggtcggg aaagtaaaga tcgtcgcgaa gatcgaggac caggaaggag tccggaacct
    11401 ggaagcgatc atcaaggaat cggacggcat tatggtcgcg cgcggagatc tcggcgtgga
    11461 gatcaacctg gaagacctgc cgaatgtcca gcgcaccatc gtgcgactct gtgccgagta
    11521 tggcaaacgc gtgatcgtgg cgacgcatct cctcgaatcc atgatccaca acccgcatcc
    11581 gacccgggcc gaggtcaccg acgtcgccaa cgccatctac gaagaagccg atgccgtcat
    11641 gctgtccggc gaaaccaccg ttgggaagta tccggtgaag tgcgtcgaat tcctccgcag
    11701 gattgcgctg aaatccgaaa cgatacccgg attgcagttt gcgaaacatt tgcgcaacgc
    11761 ggaaaacaag caacaactgg cggcggcggc cgtgcagctg gccgaggggg tcaaggccaa
    11821 aggcatcgtc gtgatcacca gacgagggct catggccgat ctcgtcgcaa actgccgccc
    11881 gttcgccacc aacatctacg cctttaccaa catgagccaa ccgcgacgga ccatgatgct
    11941 caatcgcggc gtcctcccct tcaagatcga tttcagttca gaccccgaaa aaacattaca
    12001 aacggcgttc cggattctga aggaccgcga ggatttcaag gttggggaca aggtcgtcat
    12061 catctcggac gtccttgctc agcaacgggt cgactcgatt cagattcgcg acgtcccctc
    12121 tgacgacatt ctgcccgacc tatccgcggt gagtcattga gcgaactgcc ttcgaaagac
    12181 gtgtgtgaat taacccatcc ggggaatgat cgtggactgg tggcgggcat aaagcggcgc
    12241 cgggcattga actcacgctg cggcccggtc acgcacagct gacagctaag aaaccggcgc
    12301 cggcactcct tcaccccact ctacggggag ttgcgccgcg cacaagagcg ggacagattc
    12361 tcctcctggc caaggccctt tgggatacgc gttgttgcct ctcccccacc ccgcatcacg
    12421 cagggcggag gagaggatgg tctgcctacc gaacctccaa cttctttgcc gccactgacg
    12481 cagcctttcg cagatgctct tcaggatcgc cctgcgcgat ggccgtaatc cgctgcttga
    12541 actcagtccg attcaacttt cctgccgtca tcagcgccag cttccggaca cccgaatcgt
    12601 ggtcattgag cgatttcatg accaagtcgg tgggagcaac cgcggccaga ttctgcagcc
    12661 cttgagcgac cgcaatacga acctgcggat catggtgatc ggcggccttc cttaatacct
    12721 caaccgcgcc tgctccgccg atcaagctcg cagcgtagac ggccttggac gatttcagcg
    12781 gatcgtcggc ctcggcaatc agttgtaagt gcggcagcgc ctccggcccc agcagagcca
    12841 gcgcttcgta attgggctcg tctttgtcga tttcagctaa tacctgctgc attgtgattg
    12901 gcatgttctt gtctcccggc taagtcgtaa aggagctcgc caacatcgtc gtcttctctg
    12961 aggcaataat gtccggcgga gggttagtaa tattgtcggt tcccagtccg gtcatcagcc
    13021 ggttgttatc actgacgtgc gacagcccca agacgtggcc gatttcatgc gctaaggtcc
    13081 atgcgctggc cccgctggcg accacggccc cgggccggtt ggccggatga ctggcgcagc
    13141 cgttgaacgg cggctgcgtg ctgcgaacga agtaggcaat cacatccttc gccgctgcgt
    13201 tattccggtt ggcaaatagc tgtgtttgct ccgcggtcac cgtccccatc gtacaggcac
    13261 cgacatcaat atcgttcagc gccggcagat tcagcacttc aaacgacaca tatttgaggc
    13321 cgacttgcgc ggtcgcatag gtcagacgca tattggaaat catctgatta atcgtaaatc
    13381 gagtgggcgc cgtgagacac ttgacgtgca accgcaccgt ttcacggcag gccagcgccc
    13441 ctgtccccgg gctccagtaa atagatccat gctggaattc gctatatcgc ccggtactgt
    13501 cttccgtact caattcatcg ctggtggggt atcccagaaa actgcgctcc catcccatgc
    13561 ttgcccatag agcccgaatg gcgccatgca cctcatgagc actggtcgcc ggcgtccaat
    13621 agactgaccc gccttgaaaa tgattgtacc gcccgacccc atccggcgtc gtcgtctcat
    13681 ccgtcaacgg gtagcctaga aagctacgtt cccaccccag actcgcccac ttaccccgaa
    13741 tggcgccatg cacctcatgg gcgccggtcg tcggcgtcca atagactgac ccgccttgaa
    13801 aatgattgta ccggccgatg cgatccggcg tcgtcgtttc atccgtcaat ggatagccca
    13861 ggaagctccg ttcccacccc aaactcgccc acctaccccg aatggcgcca tgcacctcat
    13921 gggcaccggt cgccggcgtc caatagatcg acccgccttg aaaatggttg taccggccga
    13981 tgcgatccgg cgtcgtcgtc tcatccgtca acgggtaacc caggaagctc cgttcccacc
    14041 ccaatttcgc ccatctgtca cggatgagtc cgtggacttc atgagctccg gatgccggcg
    14101 accaatagat ggagccgctt cggtaatgcc ggtaatggcc gatcccgtca ggggcgacga
    14161 gttcggcggt cgtcgccggg cccagccaac cagcgggccc tccaagagcc gcatacttca
    14221 catcaatatc tgacatctgc actccctccc tcatcacaat ggttattact aacagtgcac
    14281 ggagcagcga gcgtcgggtg gagtcttaca gtgctggccc tttcaacact tcttgtcccg
    14341 ctcagcctgg attgcatctg atggcagtac ccggtctcta cccgttcagg cgctcgctcg
    14401 ttgaaatctc aacttgtcat agagcaagcg gaatgcccac gtagaacagg cgcaggacag
    14461 tttctcaaac tgaacaattt cagcgccgta ccagttgttg agcagcgccg ccgcgcgaat
    14521 catcaccttc ttctgctgag gtttgtatcg aaagagattc acgacatgta tccgaacagg
    14581 ttcatgattc ggctgacgaa aaatagtggg agaaacacat ccacggttga gagtcccgcc
    14641 ggaacggcga cggctcacaa ccgatgctgg aatgcgtgca gatcacacag gagaggagag
    14701 atgaaggggg tcagagatag ggatggccct gcaaccacag atgggcctac agcgctcgtt
    14761 tctaacaacg atctgcgctg acgcattcct gtccgaggtt cctcagaacc atcgggccgt
    14821 ctgaaccgcg caggtgatgc tgccgactta ggcagtcacg agcgtttgct tcgagattcc
    14881 tttcaacaac caggactggt tctcataaac aaaccggttt cgctgctgta ccaaggtggc
    14941 cagaatctgc tcccggatct gccccaggcc tgagtactgt tgacactcac ccccgccttc
    15001 ccgaaaaccg tcgcaaattt cttctaccac tgagaacaat atgttgttgc tgaatttggt
    15061 cacggcactc aatgcagttt tgtatgcgac aaggtctgca gcgggctcga aaagccgctc
    15121 catcacaaag acctcatgcc acgcatgatt gatcatcgcg gccacacaat ccgatcccct
    15181 gacccattcg ccgactgttc gtcctattgc ctcgtagcaa cgcgcaatcc ggggatcgag
    15241 aaaatcgtag tccgggttac acacgtcgcc gttgaaccgt ccctgggcca tcagcgtctc
    15301 cttgataggc gtgccgtcat agggcaacat tttgcagaac accgccgcga cactcccatc
    15361 gtcgacaatc cggcgcagaa accccacatt cgtttcaatc gattcgaacg tgctggatgg
    15421 atcaaacagc atgaagccga attcccacat gaggcccagc gacttcagcg tctcaacggc
    15481 ctgcagattt tccgccacgg tgatttcctt gtgaagcaca tcgagtccat cgtccgatcc
    15541 agactccagc cccatgtaca ccaggtacaa cccggcatcc cgcaaggtcg caaacaactc
    15601 cggctcaacc gcatcggccc ggcaactgat tttccagatg acttttccga ccaggtcctg
    15661 gcgatacagt tcatcgacca gactgagcgc ccaccgacgc cacgacgggc cgaagagggg
    15721 gaaatcatca tcttggaaca ggaacagggt aatcccccgg ttctgataaa gatccttcat
    15781 ttcgcggacc acttcggtta ctttccgaat cctgacgacc cgtcccggag cggcccgata
    15841 aaacatatga atggaacaaa aggaacaggt ccgggaacag ccgcgtgacg ccaggatggg
    15901 cattgttcgc tgaccgagga tcgccgtcgg ctcatactgc cggtagggaa agggcaacac
    15961 atcgaggtcg tccaggagat gccgaagggg atttgacacg aatcgcccgt cccgacgata
    16021 cgcgagccca tcgatcctgc gccaatcctg gtcatgaatg agcgattcca cgagctcgag
    16081 cagggtcacc tccccttcga atcgaaccac gctgtccacc tctggaatca actcaagcgt
    16141 tcgctcgtga ctcagacttg gaaagtgccc gccgattgtg aaatgacacc ggacgccatg
    16201 gttccgcagg tattgcatca acgcacggaa gcgaggcaga taaaattgaa agatgagcga
    16261 aaacccgacc accaccgggg cttcagcttc aatcgtcgcc aagatctgct ccgccgcatc
    16321 ttcgaaatcg agcaccttca cacgatgccc tcgtcgcatc agcgttgcgg ccagataccc
    16381 gattccaagg ttgccctgct tctgaaatcc gatcaagacc acgggtctgg tcgcgtcctg
    16441 ttcgctcaag gcctgcagag agggcatggc ccctctctcg tccagcagag tgagtgatct
    16501 catagcctcc tcccaaggaa tggccgccac aatgcgtggc tacaccaggc ccgatcattg
    16561 tcatgtcgaa cccggttcct gcacagcaaa atcctctgaa aaggagcggc atagggccgg
    16621 gggtggcgtc cactcccttc cactgaggct cttgcacgta gctatttgcc gccgaacttc
    16681 ttgaccattt ccatcgactg ctcaatcgct ttcatgttct gctgcatgat cgcccgctga
    16741 ttctccagct gaatcaacgc cagctgactc agcgcttcct tcggaaggtt tgcacgttcg
    16801 aagaaccacc aaggcacagg atcccaattg atcggcactc gccacttgaa tacctccggc
    16861 aatggcagtt ccggattcat gatgggcttt tccattcggt cctcctttgg tgagtacgct
    16921 caatattgcg atcgtcgtga tcgcggtgtc cccccggcca cgcagcacac aatgagttac
    16981 gacatgtgtc gtcaccagca gaggaacaag caagatatcg gccacattgc agcggtcatg
    17041 tcatttacct acttccaatg atttggacgg tctgcagtcg gaaaatgatc aggccttcgt
    17101 gtatacgatg cgatactcac gtatccaaat tgatacgtag acgaatcagg aaaatggttg
    17161 tttaagagaa ggcctactgg cagggtgcca caccgacgac ttcgattggg acgttccgga
    17221 ttcgccgacg atgtgcctct cgcaagccgt caaggtgatt ccaggtgagc tgctggttgt
    17281 tgtaggcttc gagtcgatgg ggcggacaga tccaaatcaa tcgggtggag aggcccggga
    17341 ccagtacagt ctggagcgcc agcaggtgtg ccggagattg cccgtcacgt catcacctgt
    17401 tgatggctca cgcccatcac gcgcttcgat tgaaaggacg acacgcatta gcgagggttg
    17461 cactgcagca acgtttgctc aaaccattac aggctgatca actgacacac cgcatcgcac
    17521 atgaaatcgc gacgggtaaa cctcgaaaga cacgctcacg cgtatgcaaa cgatcacgag
    17581 gccctaccca cgcggaacac ggtatcgcaa ctcagcgaag ccgggcccga tcgcgcgaac
    17641 cgactccagg atgagggaag gcgacgtgat ccggcgaggc aggagcggct tgcctgatcc
    17701 gagggtgacc gaaccaacct gcacgatcag ttcgtcgaga agtccggcgt catagaactg
    17761 cccgaccagt tcgcctcccc cgaccagcca cacgttccgg ccacgcgccg ctttcttcat
    17821 gtcagcatga accgggagaa cgtccccgct tacaaatcgg atgtctgtac cttccacgga
    17881 gggaaggcga cgccttgaga acacccaggt gggctgagca tagggccagg cgaccggttc
    17941 gcgcccgtcc ggacggatga cgtggcgaag catccactca taggtggcag accccatcgc
    18001 caaggcgccg acctccttga tgaaggacgg ataccccgtc tcgttgatat caccaagggg
    18061 aaagagccag tcgagagaat cgtcgagcgt tgcgatgaat ccatcgaggc ttgaggcagt
    18121 gtagtactgg gtggtcatag gcgatacatc tccttgccgg cactcaccta ttcagcttgt
    18181 tcggcaacac gaggctggca gaagaattcg tcggacggcg caccttgcga tctcacaatt
    18241 cacacgctca cccttgtcgc caggatgacc cgccgaacgc gcggactgcc agataggcca
    18301 cgttgcgctt ccaataggcg accttgcttc gcgtcatcaa ttccagaaag aggtcatcgg
    18361 cctccttccg atcaaagcgc ttgtcggcgg gcgtgacttc gccatcaggc aatgccacgg
    18421 ttccggcaga gccataaatc aaatcgtgca cgataggcgc aaccagcgag agttcaaatg
    18481 acgcgatgaa gccccataac aaacgtggaa tacttgccaa gtcggtcttg aagccgcgct
    18541 tggccgtcac catgaaccca tccaatgccg cacaccgaca atcgtcgagc aagacccatt
    18601 cggcgttgtt gtacgtcaca gccggttcgg gcaactccag cgcccggcgc agattcgccc
    18661 cggagccgac ggctggtttc gccgctgtcg cgttcgaaat atgtgactga atctcgcggg
    18721 tggaaatctt ccgattccct tcgccgatgt cgaagctgaa cgatttggtg agagctgcca
    18781 cgagactgtc gttgtcggcc aacgccggag ccttgcccgt cgcggaccgc tgaggtggag
    18841 tggcacgctc gacgaccttg tcacgactga ccgtgaggga catcgatcgg tcgccgtcgc
    18901 tcatgttgat ggtgatcgtg tccccattct cgatttgctg gtcgatccgg gcaaattgct
    18961 cgatgagttg cgtgaggtac gcattcgtgt gcgtaaattg cgcgtggacg atgtccggca
    19021 tagagtctct ccttaagata tcctcagcag gctgccggct cgttccgacg cactgccggc
    19081 ccactcctcc cccgttcgca cgaccggaga ggcggcctgt cctcgctgcg cgggctagtg
    19141 gggaagcgga agggtcagca ggattccccc catcacgtca tgcggcttga aatctttctt
    19201 tggactgttc ggatgcgcgt tgtggcaact cacacaggtc tgggacaggg ccttgtccgg
    19261 gtagagagcc tggaagacgg gtctgcctcc ctcggtcgtc acaccggtgt ggggacgatc
    19321 gggattgatc aggatcgcgg tcagaccggc ccgctcgaag tcggtggccg gacgattcgt
    19381 cttattgatc gcccaattgc tgatgagccg gaatcgaatt ccattgcgtt gctgcgcaac
    19441 gagccgaccc gattcgagca agaactgagc aggaagggga agcctggagg tctctctcca
    19501 gttttctgcg gcaaagacga ttccccgcaa ctgcatccgc tccacgatgt cggtggtgta
    19561 gaactcccga tcggcctgga tcacgctata cacataatcc gcgaccatct cggccggcaa
    19621 acagggggcc gtccccgcct ccttgctcgc ggcactcatc gtccactgac tcaacagaac
    19681 ggtggccacg atgccgacta cgaggccggt gaaaaatcct gtacccttca tgctgcaccc
    19741 acctttcttg tccgcgcgtg gttgccttgc tccccaaccg cagccataca cccgcccggc
    19801 cagaacttgc gggtcgcctc ccgcaaaagc atacgccgac acagcagaga aatcgagatt
    19861 atggaccggc ctgtttccga accggtacaa tgccggtggt gatgtcgtct gaacaatgtg
    19921 attccaatcc gtggagagaa gaatcagcag tcgcttcagc gggaatgcag ccaatgacag
    19981 gcaaatttga tccggaatcc catgagtggg ccaggcacga gcacgtcgtt gtattcgatg
    20041 gtgtgtgcaa ttggtgcaat gcctgggtga actttttgat cgatcacgat cctcacgaga
    20101 cgttcaagtt cggcacccta caatccgacc ggggacaaca gattttgcat acactgcagc
    20161 tttcgaccga agacttttcg accttcctcc tgctggagca ggcccgcgtc tacaccaaat
    20221 ccacggccgc gctgagaatt gtgcggcacc tccccggctt ctggcctcta ttctatctcg
    20281 gcatcctgat cccacgcccc ctgcgtgatg cgctgtacga ttatgttgcg cgtcatcggt
    20341 accggtggat gggcaaaacc gatgcatgcc gggtcccctc cccggaccag cgcagacggt
    20401 tcgtgtagtg cctgcgggca gagagtttcg ccgcacgcag cgcacgttcg tgccttgagc
    20461 cattcacggg aacggcgtag actccgcttt gtaacgatcg cgccggcatc gaggccgtgc
    20521 ccattctcat gcctgatgaa gaggaagaac ctatgaacca cttgttcacg gcatccatat
    20581 tcgtcgccat cgctatggcg ctgtctgttc catcaccggc ggtcgcacaa aactttcctc
    20641 cgtctgtcac acaaaagatt cacgcggcgc agaagcaggt caaaacgatc ggcctggagg
    20701 agtatcgaaa agtcgtggag agtccgggcg aggcgttaat tatagatgtg cgccaaccgc
    20761 aggagttcgc ggccggacat gtgccgggcg cggtcaacat tccccgcgga ctcgtcgaat
    20821 ttcgaatctg gaagcaggtc gggtttccag ccaacccgga cacgaacaga cctgtctacc
    20881 tgcaatgcca aaacggcaac cgcgcgtccc tcgcggccca gtcacttgcg gagttggggt
    20941 tcaccaatac caccgccgtc gtgatgagct tcgatgagtg gcaacaggcc ggccatcccc
    21001 tggtgaagta agccatcccc tcgcgcgcga tgagcgcttc gtgacaccac tcacggctcc
    21061 gccgacacat gccctgttgg tttgtttatt caggcaggaa ttattcctct ctgacacccg
    21121 gcaaataata tcaaatgcgt cgcatccgtt gtgaacgggc actgcatcaa tccatcgcca
    21181 acagccggta agtaggtcgg ccgttccgct caatgcgaag cgcggcacgg ttcggagagg
    21241 ctcaggacga cccgataggc atcggaatga tagaactgag gcagtgtgaa cgagagctcc
    21301 tcattctcca ctgagagagc acgatcgagg gtgacaacag gcgcttcgag gggaccggat
    21361 cctacatctg gtatttgctc agcgcagaga tgcaccgtcc cgctgtgctg cgaggaaaac
    21421 agacgcccga tattcttcag cacgacttgt gtgtcgccag gcgaatcgtc ctcgtatttg
    21481 cccaacaaga gtcgtaacgt gccggcggcc ttatccagcg cggcgagggc atggagcttg
    21541 ggactatccg tcatcgtgta cagcctgaca ccctccatct ccgcataggt cttatagacg
    21601 tgccaccccg gccgtttctg cttccgatcg acatacaaca atccgtccag cgtagggtta
    21661 tcacaggtat tttccacgta gctccggctc atgtcctgag gatcaggcca acaggagcgc
    21721 atcgcgctga ggatgtccgc ccgctcatag gcggcaatca cccccgctgt ataaccggca
    21781 ctcgtccagg actcctgccc caggatctcg ttcactgaaa tcggccgcgc cagaccgtga
    21841 gccttcaaat agtcgcgcag atcattcacg tacctgatgg catccgatgg atccgcatat
    21901 tcatgatagg ccacgacgtc gggaaagacc ccctgcgaat cactccactc cagaaagggc
    21961 ttgagtaacc ggtatcgact cacgctcggc ccgacgatct gtgcgccggg aatccgctgc
    22021 ttgatgcgct gataggccac ccgccagcct tctttgagtc gttcgctgtc attgagagga
    22081 accgctgcaa gatcgggttc attgtaaata tcccagatca ccgacagttg ccgctgcagc
    22141 acttgatcga tcacatgatc cacatgcgcc aaccagggct catagttccc gtcgagaccg
    22201 atctgattga tgtcaaaaat ctgcagggcg cgatgctcga agaccagtcc gagggtcagc
    22261 tgaaactccg tcaccccggc ctgtttgagc cggtcatagt tactcaacac atatccggtg
    22321 tttccgcgaa aactggtggg cttcaacgga agaatcagct ccatcggcgg gtctgtcggt
    22381 tcgatgctga ccagataccc gttagctcga tggttcatcg ggccctgctc gccccccaga
    22441 tcgaccctga tagtgccggc cagactccaa ggcgcgcaca acaacagtcc agctgtcagc
    22501 aggccaatga tcgaggcaag tcgcacagac agggctcctc ccatgacgcc ttccaatcag
    22561 aaaatccaac gatgttcggt tgtactgccg gaaaatgccg gcggtcaaga tggagcgggt
    22621 agaatcaggc cgcgatacag ccttcgcgcc atcgtggaag ctggggcatc aactgttcac
    22681 gcgccagata gtgctgatcc agaccgggga cttcggtcgg gttctcaccg ggacgggcct
    22741 gggcctcagc catggcattc gccacatgaa cggcggtcac ggcacagaat ccggatccct
    22801 tgtagtcgtc aggatggtgg tgataggcca ccgcttccac aatgccgtcg ccgagtcccc
    22861 agattccgag cagataggcc ccgacatggg catgatcggc cccgaacacc tcccgctcgg
    22921 cttcccaatc aggcatctgt tttgactgca tcagctccag gactcgcgcg tagtcatcgg
    22981 gtttgttggc caccaatacc aacaccccga cgtcgtggag caaggcagcc gtaaaggctt
    23041 catccatcac ggcttgactc gcaccggtct ccttcgcaat tcgtcgcgcg caggtgccgg
    23101 cgagcatcgc atgccgccac aactcatcca acgaaaacga cgggagttgc gcctggtcga
    23161 actgcgcgaa gacctgactg gagagcacca gcgacttgat tgtatcgaat ccgagcagcg
    23221 ccaccgcctg ctccggatcg gacacatggg tgcgaaggcc gaagaaagcg gaattcacca
    23281 gctgcaggat cttcgtgacc atgccgagat ctttggccac aatgcgggac gccttcttca
    23341 gcgaggcctg cggagcctgc atctccttca tcaactcctg atagatcgac gggagactcg
    23401 gcagcgtttt aatctcgtta gccaggctgc gaagcgcggg attggccagc acgtcccgta
    23461 atgcctccgc cttctcaatg gtcgctttca gcgtggcgac atcgaacggt ttctgaagaa
    23521 atcgatgtgc gaccgacgcg gaccgcagat aattggcagg gctctggtct ccggacagaa
    23581 cgatgcgggt cgcctgtgga cagaccttgc gcacctcgcc gagaaattga gcgccgtcca
    23641 tccccggcat cgtcatgtcc gacaccacca cgtcgcacgg ggtctcacgg atggcggcca
    23701 atgcggccgt ggcggaggga tagaactcca tcgtccattc ggcctgcatc ggagccagtg
    23761 tccgctgcaa atgcttcaaa atctccggcg catcgtccac aaagatcaca cgtcgcatcg
    23821 tcccctcccc cccggcagga tcttcctcct cttctcatcg gtcagccatg ccagaacctt
    23881 gagtcggttc agggaagccc ggaaggaagg cggcgtgcgc gaatgattcg tgacaactac
    23941 tgagtcagag agagggtgac ggcaggagga tcagttgcga tcgagatccc aacttacgat
    24001 gtcggcgttt ttcccttcgc ctcccgggac cccgtcggcc ccgaaggaaa tgatctcgaa
    24061 ctcgccaccc tgcgtggacg gagtcgtata tttgtaggga ccgccccagg gatcgaccgg
    24121 cactttggga agatacccgc cggccttcca gttgggggca gcaggacctg acgcaggtcg
    24181 ctcgaccagg gccttcaagc cctgctcggt cgtgggaata ttgccgttgt cgagcttgta
    24241 gagttgaagg gcactgtcaa tattcgaaat ttgagccttg gccgcggctc gtttggcatc
    24301 ttcggtgcgc cccatcacgc gaggaatcaa cagcgtcgcc aggatcgcga ggatcaccac
    24361 gacgatcatg acctcgatga aggaaaatcc tccgcaggat ctcagtctat tgagttgagt
    24421 gccccaagaa tcggtcaccg ccccggtacg acgagagcct gaactattca gatagtgacg
    24481 ccatcccata tgcacgctgc tccttctatc gacagacctg gaggaccttg taccacgcag
    24541 actcacgccg atcgggaatt gtaacaggaa cttctgtctc actgtagccc aattcattcg
    24601 gaccattgct tcgcgcattc gctagcccgc agtattcatg acggttcgtc gtgaaatcga
    24661 cgagtcacgt tgcgagaccg gcgcgcggag ccgggaggac ccgaggcgta ctcgtgcagt
    24721 acgttgaggg tccgaggggt gagcccgccg gccgaaggcc cgtcgcagca gtgaatcggc
    24781 gattgcagca gaagtgctca tgaataatgc gggctagggc cgcgcgatct gaaagagcat
    24841 gacaccgggc ttcagctgcg cgcggggaag cacgctctga tgcatccgaa acgtcggcag
    24901 cgatgccacc ggcgtgatcg cctggacgtg catcccggcg gctcgacatc cgtccgctac
    24961 cgctgaaggg ccgacgataa acacaacggc gccgggcagg agttgccggg caagttgctg
    25021 catccgcgca gccaccgcat ccggtttgtc atagtggttg aacggtgtcc actcgtaggc
    25081 gagatcgtat gttctgttgt tcagatctcc cctctcatcg acacgggcac tcaggcgcac
    25141 atgggagagc cagtccaatc gttgtgcgcg agcccacagg ttccataaca tctgcgcctg
    25201 ccgctgcgcg aacgccgggt ggtcgtagag cacggtgtag gcgcgggggc gatcgcctcc
    25261 gatacaggtc gtcatcgccg cgtcgaatcc gctgatcaac tgatgccctg cccgccctac
    25321 ctgcgcagcc aacgggcgat cctgctcaat gaggtcctgc agatagtccg ctacgaacgg
    25381 ctgcgtcggc aattcaccga tctcacgttc atcgtccgga tagagcagca ccgcgccaaa
    25441 cgcctcatcc ggactcacca ccggcgcggc ccctccgaat acagcagtcc aatcgatacc
    25501 accccgagca gcgccgacca ttggcggtcc ggtcacagtt ccccagctgt ggcccaacgg
    25561 aagcacccgg cgggtacctc gatccaccaa caggagctgc ccctgttcaa ccgccactcc
    25621 acgctcgcag ggcgcagcgc cgcaccggtc gaacggttga taggagagtc cggccgggtc
    25681 atcccagcag gtggctttca agattcgttg cccttgaatc gtcacacaga gcttctgcgt
    25741 cgcatcgaca tgccgtctgg gtggggccga taccggaagc caggtcacca tgtgcgaccg
    25801 gggaggatcc atgaacaggg gaaagggatc gcccccgccg gggatctgcg gggagaaaaa
    25861 gttgctgaac agccggtagg ccgcctcgga cggataagtg gggatgcctc gataccgcag
    25921 agcccgtggc gcgggtttcg tccggttctg atcgtcatac agtccacgaa tcaattcaaa
    25981 cagcgccgac ccagtgccgg gcagcagcga gagaaacagc tccactaccg gaagaaagtc
    26041 gatctcctcc caatgcatgg ctcccataca agccatgaat tgcttggagt cgaacgtccc
    26101 gtccggcaaa aaatagatcg catccttccg gtgccggatc gtggcggcgc ccgtcgcccc
    26161 gatggtgaca tcctgatcgt tgtagaagaa ccggacttcc tccagcggaa cccgcatcgc
    26221 ttgcgccgcc atgccacgaa gatcgtccgc cctcagtcgt tgccaccccg gcttgcgcga
    26281 caagtccagg gtcatggtat tcaccaggcc gctgggagtc aggccgaccc aggcccccca
    26341 gtcgagatgc acccttgccc gcaggaggcg gagcgcgcca tcgacgggcc cccattcaca
    26401 ttcatgcaag ggatgaccgt ccggatcggt cgcacagaga cggcgaccat ggcttccata
    26461 gacgacgaga tggccggtcg gttgttgaga aacggatccc gccacagccg tcaggtgtgg
    26521 caggtccgtg agattggaag gaaaggtgag cgtgccggga cgcctgagaa tctcgtgtac
    26581 caaggaatca ggcggcattt actcgcggat ttgaccgctc ccgagcacca caaattttga
    26641 acaggtcaat tcttcaagcc ccatcggccc tcgagcgtga agcctcgatg tgctgatgcc
    26701 gatttccgcc cccaggccga actggtagcc gtcgttgagc ctggtcgagg cattcacgag
    26761 caccgcgccg gcatcgacct cgcgcagaaa ccgcattgcg cgcgcatagt ccttggtcac
    26821 aatcgcctcc gtatgttggg atccgtaggt ggcgatgtgc tccaaggctt cttccatgtt
    26881 cttgaccact ttgatcgcga ggatcagatc cagaaattca cggccgaaat catcctcagc
    26941 ggccggcgtc acctcaggac agagctggca ggtcttcgcg caaccacgaa cgtccacctt
    27001 cgccgctttg agtttctcca ccaggagcgg gagccaggtg cgcgcgatac tgtggtggac
    27061 caacagggtc tccatggcat tgcaggtcga cgggcgctgc actttcgcgt tcagacagat
    27121 ccgctcggcc gcctggggat cggcatccac atcgacatag gtgtggcaga ctcccttgtc
    27181 gtgtttgagc accgggatcg tcgcatgttc ggcgatcgtc ttcatcagcg attccccgcc
    27241 tctcggaatg atgagatcga tagaccggtc ctgtttgagc aacagctgga cgacctcacg
    27301 ctccgcacga tcgacgaatg taatcgcgcc cgccggcacg ccggccttct ccgccgattc
    27361 gctcaacacc ttggcaatgg ccacgttcga atgcaaggct tcggatccgc ctcgcagaat
    27421 acagacgttt ccggacttga gacacagggc ggccgaatcc gcggtcacat tcggacgcgc
    27481 ttcataaatg atcccgatga ccccgatcgg cacacgcatg cggcccactt gcatcccgtt
    27541 cggccgcgtc cacatcttcg gcgtatcgcc gagcggatcc ggcaatcgcg caatgtcacg
    27601 aatccctgcc gccatctccc ggatacgttc cgccgtcagc cgtagacgat cccccaaggc
    27661 ttttcgctcc ggcgtggcct cgaattgttc gagatccttc tcgttctcgg ccaacagctc
    27721 cgtctcatgt tcttcaagcc cgtcggccat ggcttccaag gcctgattct tgaccgccgt
    27781 cggaaggctg gagagccggc cagccgcccc cttggccctt ttgaccaggg tcgcgacata
    27841 ttcttccgga gtgagtactt caacgggagc atccagctcc ggaaccgggg attcggtgag
    27901 atcttgttcc atgtcgtgaa ttcctgctgc tggccacacc tgaggcccga tgcctcagca
    27961 tatcggccga tcaagatggt gaacgaaggc gcggcgatct atagccagac aggatactgt
    28021 ggggttcgga gtcgggtcaa gggttgcgag caggacccgg tgtcggcgga gcacccggag
    28081 cagacccctg cggccctggt tgcatcggca ttgtgccagg agccaggccc atcatgggcg
    28141 gcatcaacat ggacatggat gagggattgg gatgttgaaa catccagtcg tgatacgttt
    28201 tccggccttc gaagtgacgg accgcgaggg ggaaatcgcg ctccttgatc ggtttcgctt
    28261 tacttttgct ccggacgccc atgatgccgc cggtcggcgc gcgcatatac tcccaatcac
    28321 cgccgctcat cggatcttga tagaccctcc gcagatacgg cttgggcgtt cgcgtcaact
    28381 ccgccaggga ctgaggatag atttcggagg gcaggatgcg cccgatcttc atcgtatttg
    28441 agtaaagggc aatggcattc tgaatttcga tgcccttggc cataagatcg gcttctttct
    28501 cgcgctgaac catgacggtc cattggcggg cagcggcgga cacggaaatc ccgatcaaca
    28561 cgatcgaaaa catcaaggcg agataggtaa tcccgtcagt atttctgaga tggccacgca
    28621 cccccatcac ctgcctaatt gggttgcccc tcagcccctg gcgcatccgt gaccggaatc
    28681 acctgcgtaa tttttgaggc cacatcctgg agcgtgatct cactcatcga gaccgccttt
    28741 accaacacct caccggcaat cacttcaccg acctgcacca cgtgcatctc atcattcttc
    28801 acgaccacgg ccatgttctt tttcttctgc gagccccctc ccaccgccat gaaccccacg
    28861 tagcggaact ggttcaattc cgtcgcaatc cgcagccgtt caatttcttc cggcgtcggc
    28921 cccttcggct cttcgaccgt cggctcttcc tcctcgttct gcggtgcttc caccaccggc
    28981 tcccctgcag gcgacggagg cttggcagga ccgcgagcac gaccgcgacg agaggcggga
    29041 ggcggtacca caaccggctc ctggggaagg agactggcga aaatattgcg cggagtcgca
    29101 aacgtcgctt cccgctgccc cttaatggcg gccagccgct caagatgcac ttgtagattg
    29161 ccgccggcag cgacggccgc tgccggcgcc ttgcccaccg tccgctgccc gctgacatgc
    29221 gtcaggggga ctcgttcctg ttcgctgaaa tggccgaatt gccagacgag caacccgatc
    29281 cagaggacca cgagggtcat cagcagcaga ccttgatttt tgctgactcg attcatgtgg
    29341 gttacggcgt cggggactgc aaatgctccc cgcgcagata ggtcgaaatt cggatattga
    29401 aggtcaattg ctggtcctgc acattccccg agcgaatcaa gttgaggtct tcaatgaaca
    29461 acagttcctc ggccgattcc aggttgtgca gaaagcggcg caaatcctca taccgaccgg
    29521 tcaccgtccc ctgcaggacg gctttcgtcg cgtcggggac ggcagtcttc tctgtcttat
    29581 aggagagggc gggcaacgtc acctgatcgc gtttcgcctc ttcggtgaca ccgagggcca
    29641 gcggcgcaaa atcacggaac aacggcaaca cggcccacac ccgctgcaga tccgccttcg
    29701 ccttcttggc ctccttgtgc cgattcaact gttggcgggc tttgagccag tcggcctcaa
    29761 gccgcacctg ctcctgctct gccgccccca agaccagggc atgaatgccg taactcagca
    29821 cacacagccc cgccacaaac gcgacccagg gaagaatggg tgcatagggt ttccgccagg
    29881 tcagttgaag acgatcgagt cctggaaaca tcatgggttg cgattccgat agcgcaacga
    29941 gagatcaaac tcgaccaatc cgctcgccat gacccggtgt tgcccgagca caggttcctt
    30001 gaattgggga tgatcctgaa acgtcaccgt caatgtcgtc acatcttcca atgcccgggc
    30061 ggaaccggtg agcatgatcg tggcattggc aggatcgagc cgaatgctgt taaccgcgat
    30121 gcgttgagga atcgcccgct ccagttccgt cagaaaccgg gtccaggaaa agctgcgctt
    30181 ctcgatcagg tgattggcaa actcgatctc cttgggcaga acttgcaagg cggcctccga
    30241 cagatccagg ccttcctgct gcgcctcttt caacaggtcc cggtcccggt cctgcacctg
    30301 gttcagagct gactccatgg attcgacgtc ttgccaaacc aaccaggcct gcgaaatatc
    30361 ccacagcatc gccaggccga tcgcccccgc cagtaacatg atcaccagcc tggctggagc
    30421 caggtaccac cgataccggc tgctcatatt gatcgcgaat gcgccgtgcc tagcagctgg
    30481 ttgcccgaca cgcaccgcga catcccgaat cgccgacacg aggttgtcca acgacatggc
    30541 ttacacgacc cctgcaatcg ccggcaaggc ggcactcgtc cgtggtcccg caatggtgcc
    30601 tccaccgtac tgttgcacgc ggtcccattc cagttcctgc accggcacgc ccaatccctt
    30661 ctccaactgc tgccgtaacc cggcgcccat ggcttcatcg gcgatcagca ccgcttggct
    30721 gatggccaac tccggcgtct gctgctgaca ggcatagata gagtccgcgc actcttgcac
    30781 gatacgattg agcgccacct gctcggaacc cggtgccaag gcgccccccg agccgatccc
    30841 gccctgcagt ttcgaccgga gaaagaccaa cgtcccgttg tgaaaaatca gcgccgtgac
    30901 tccgccgtcc gtggcattca cccataaaaa gtcagcggtc gaacgagccg cacgccccgt
    30961 cccgtgcgcc cataagttga acaatcgcaa acttgcgaca tccacttcct gtggaatcaa
    31021 ccctgccgct tcgcagaccg cttcatactg agtcagcacg gcttcttgca ccacgaccgc
    31081 cagcacggta cagggcccat caccggatcc gcccggatcg gacagcacct gcgaaaacac
    31141 tttggccccg ctaagggaca ggagctgctc ctgccccaat cgccaccgca ccaacgcatc
    31201 gcgctcatcg gagctccagg gaaggtcctg caatcgtaac accgccagtc gtacggccag
    31261 atccggcaga atgatggtcg ctgcgcgcgg gacgcccgga gtggaagagc gaccgggggc
    31321 ctgagtggtg gccgaaccgg caatcgcccg gatatgcgtc tccagttcgt ggggagagat
    31381 gatgttttgt tccaacggag acaggcgcac gatcccctcg ggcaacgcgg acaccacgca
    31441 ctggtaccgc ggcctgccgc gccaattccg atgcacctcc gcccacgcca gatcgcgtgc
    31501 gccgattttc aggcagtact gcggccggct ggtgatccaa tcccacatcg attatcgctc
    31561 ttcgctaaac gtcacgcggt tgatttcacg aagcgtggtc tcgccggcca gcactttctt
    31621 cacggcggat tgccgcaacg tgatcatgcc gtccgtgacc gcgcggtacc ggatctccga
    31681 caacgcacga tcggccagaa tcatttcctt gatctcgtcc gtcaggtcga gaaattcagt
    31741 aatacacttg cggccccgat agccggtgtc gtggcattcc gaacaccctt tcccctcata
    31801 gaacggcgcg ctcttgaact catcgtagtc gaggccggat tcctccgcga gcgcctggtc
    31861 aaggacaacc tgatgccggc aatcgggaca gatcacccga atcagccgtt gcgccagaac
    31921 gcaggtcaac gccgccagaa agttgtagga atcgatcccc atcgaggcga aacgtccgat
    31981 cacatcgaac acgttattgg cgtgcaccgt cgtcaatacc aaatggccgg tcaaggccga
    32041 ttgaatggcg atctgcgccg tttccgcatc acgaatttca ccgaccatga tcttgtccgg
    32101 atcgtgtcgc agaatcgacc gcaacccacg ggcgaacgtc aggcccttct tttcgttcac
    32161 cgggatttgc acgacccccg gcaactgata ctccaccgga tcttcgatgg tgatcagttt
    32221 atcttcctga atgttcattt cgctgatcgc ggcgtagagc gtcgtcgtct ttccgcttcc
    32281 ggtcggaccg gtcacgagca ccatcccgta gggtctcgta atggcgcgac ggaaccgctt
    32341 gaggtcctcc gggttgaaac cgagacgatc cagtcgcagc gtcgatacgc ccgtggtaat
    32401 cgcctcgcgg tccagaattc ggatgaccac ggattccccg aacacgctcg gcaaaatcga
    32461 cacccggaaa tcgaccgtct tccggtcgag ccgcatgcga aaactgccgt cctgcgggac
    32521 gcgccgttcg gcgatatcga ggtcggacat gaccttgagg cgggacacta acggtggatg
    32581 cagcttcacg tcgagcgggt ccatcgccga gaccagaatg ccgtctacgc gaaacttcac
    32641 cgtcgtggct cgatccgtcg cttcaatgtg aatatcgctg gcccgtcgct gcatggcgct
    32701 caacatgatc gtgtcgagga gtttgaccac cgggctttgg tcttccccga agtgatcgac
    32761 ggtcaagacc tcttcgcccc gctcgtcctc cttcaccagg accgagcggt actccgcctc
    32821 taattcacgt agcgcctgac tggacccttc gctccgttcc aatgccgatt ggatggcgct
    32881 tcgcgagctc accacatagt ggagcggacg attgagcaga atctccaatt catccagcgc
    32941 cagcaggttc tgcgggtcgg aaatcgcaat cgtcaacgcg ccggcctcgt ccttcaccgg
    33001 cacgaaagga tgtcgccgca tcaacttgac ggagatcgtg tgataaaact cggagtccac
    33061 gcggaacccg gtgagcggat catagggcaa tccatactgg gctgctaatg cctgcgccaa
    33121 ttgcgcctcg gaaatggtcc cctcttcgac gagcgtctgc ccgaaggcgg tggtgctgcc
    33181 tcccaaacgg gcaacgacct ggtccacggt ccgtctcggc aaaatgccct cgcgcaccat
    33241 cacatcggcg agcgacgggc gagtgagatg gcgttgcatc agcatattca gtccctccga
    33301 cttcggcacg ggtcacgacg cgtcgggcac ggggtcccgc cgtatcgtcg gattattgaa
    33361 tcgtcccggc catctggaaa atcggcaaat acataatgat gacgatgccc ccgaccagga
    33421 gccccatcac cagcaacaac accggctcaa tccaggtggt caactgactg agccgcacgt
    33481 ccagatcccc ttcataaaac tcggccacgt cacgcagcat cggttccagc gagccggttt
    33541 cttcaccgac cgataacatt tcaatcgcga gcttgggcag gatctgcggc cgctcaatgg
    33601 cagccgccag cgtgctgcct tcgcggattt cgttcaccgc cgacaccaac ccgcgcgaca
    33661 caaagcggtt cgagactgca ccgcgcgcaa tctcaagcgc ttccaccagc ggcgtgccgc
    33721 cggctaacac cgtggccagc gtgcgggtca attggatcgt gtggtgctcc acaagaatca
    33781 tcccgagcag cggcaatttc aacaggttcc tatccacgga gaatcgaccg gcggaggttt
    33841 gataccacgc ccgtcccatc accaccgcca caaccgccac gatcgccacc ggaatcaact
    33901 gcgcctcacc catctggatc accgagatga gcatgcgggt tgccgtcggc aaattagcgg
    33961 atgattcacc atacaccgag atgaatgtcg gcatcacgta actcagcaga aaccccacca
    34021 ccgcaacgcc gacaacgacg agaaaggccg gataggccaa cgccttggtg actttctggc
    34081 gaaggccgat catcagcttc agatacgcaa tgtagcgttg caacacctcc gccagattcc
    34141 cggactgctc accggcacga atagtggcga ggtacagatc cgagaaatag gtggaatgct
    34201 tgcccaaggc ttcggaggcc gacgccccgc cgcgaatatc ctgacggacg gcttttaacg
    34261 cttctcgaaa cggggctcgt tgggtgcgat cgatgagcag atcccagacc cgtaagaccg
    34321 gtagccccgc tttgatcagg gccaacaact cctgattgaa cacgaggaaa tcttgaaggg
    34381 gaagctttcc ccaacggcct gccgccagac ccggcacacc gaaccccgct cctcgcctgg
    34441 agagccggaa taccagcaag ccgtccgact caagcttggc tcgaaccagg tgctcatctt
    34501 cgccttcgat gtgtccctcg aaggtcgtgc cgtccgcgcg agccgctctg tatgcgaata
    34561 ccgccatgag ttcattcggg tggacacatc acgcgctgcc ccggtctcta tcgaacgagg
    34621 gttttcagcg acaccggctg catagccgcc tgctgcagcc gaggctccgg caatcggagc
    34681 gtccgtccaa caacaatgag attcgtcggc aaaccattga gtgatttcag cgcttccagg
    34741 ctgactttgt gccggcgcgc aagtcgccac aacgtatcac cgccctgcac gacaattgtc
    34801 ctgaccggct ccgcggcgga cgggatcggc atcacaatcg gcgaagcctg aggaacgtct
    34861 gagaccggtg cagcctcgcc atcggcaggt gcggattgtg gcgcctcctc ctcttcattc
    34921 tcaagcggct tcacggccga ctgttcctgt gtatcccttg gggccggcac cgctccgtcc
    34981 gggctggatg acgcacgctt ccgttgaagg cgaaccccgt tcggtggctg ctggagtcgg
    35041 ctgtgcaacg gtgcaaccgt ctgcgccacc cgctcccgtt cggtgtgagc cgaagtcaac
    35101 tcctcacgct gcaattcgat gacctggcgg gcatccgcca atcgtcgttc ggtctcccgc
    35161 aacatgccct gcagttgcgc ccgcgccact tgggcatccg ccaattcctt ccgctgctcc
    35221 tcaagctccg tgcgcaattc ggccgccgtg cgctgcgctt cacgcaccgc cgtcttgagg
    35281 gagtccgccg caacctgaag atcggtgggg gtcgattccg gacttggcgt gacccgcgcg
    35341 caccctcccc agaacaacag actcatcgtc gccactatcg agcacccgaa tcgtaagccc
    35401 acgaccgtcc gccaaacagg acccgcttgt tggatcatgg tattcattgc cttaccattg
    35461 gttgtaggga actccgttgg tgccgaccag atcggacccg gaaaacacat cgaccatacc
    35521 gccttcccct tcgtcgacca tttcctgcca ggtcgtcgtc gagccggtca acggatcggt
    35581 cggcacccgc cgaagatagc cggccgacac caacgcctca atggtctcgg gatacttccc
    35641 tttatcggca cggtgctggt cgatcacatc gcgcagcgaa aataaatcct ggcgcagcgc
    35701 cccttctttc gccttgataa tcgccgcatg gtaggacggc acggccaacg tggccaaaat
    35761 tccgacgatc gtcaccacaa tcatcaactc gataatcgta aacccatgcg gtgacgagaa
    35821 cggccatgag cgcttaccaa tctcggtatt tcgtgccatc cagtgccagc tcttgactat
    35881 gagtcgtaac atcgtacaca tcttctccgc accagtcctt cacactggcg ggatctcggt
    35941 aacaacgcag gcgccaatcg gcggctccgg tcacgggatc caagggaatc gatcgcaggt
    36001 atcgcttgat cgtcgtcccg cgcaccgtcg cctgctcccc cgtcagcttg actcccaaca
    36061 atacatccaa ggatttcggg tagccatagg gcccggtgac gtctttgcag ctcaacttgt
    36121 tcttaacgca tgccggcccc gtcagggtgt ctccgtcacg cgaccattcc aggtgaaacg
    36181 tatcgatcgc ggcccggatc accctcaagt tctgccgcag ttcgatctct ttcgtccgct
    36241 tggtcgaaac cttggtgaac ggcagggcga ctgtggcgag gatggtgaga atggtcaggg
    36301 tgacgagcag ttcgagcaac gtgacgccgg actgtttcac cgcactctca ccactcccac
    36361 ggcttcaccg gtcccctcgg gactgtcgag atcgtcatgc ttggccatct gcaggcgcag
    36421 ggacgacact cccggagatt tggcgagaaa cgtcaatcgc aacaggcgcc cctcatcttt
    36481 tgtggcgctt gcggaccgat gcaggcgcaa ttcgatcagg ccctcggtag cggatggcgt
    36541 cgccgttacc gcagctttac cggcttcggt ccccaacacc tcgccgaggg tggcatcccg
    36601 aaactcgaga atcttcggat cgaattccag cgtaaaggtc tcggtatcca acccgcgggc
    36661 ctgtgtcgcc aacacgtcca cccgtaattc cttattcatt tgagccgtga cgtctgcagg
    36721 ctggatcgcc agctgaagtt cgagaggaac aaagtcggcg ctcgccaccg tccccttgga
    36781 ttgtttcgag acgccttttt ctgcgagctt gccggaaccg ctcaacgtgg acattcgtgc
    36841 cgaaatcttt ttcggctgca tagcgaacaa gggcgacgtg gagtagacgg attccgttcc
    36901 ggaccagaag gcctgcccct gcggacccgg aagccgcaac gaattcacga tatgcggggt
    36961 aatcgtcaga atcacctccg tcgtcacccg ttgggtcttg aacgaactca acagattgcc
    37021 caggaaaggg atgtcaccga gccagggaat cgtcaccttg gtccgacgat cttcttcctg
    37081 aagaagtcct cccaggacga tggtctcgcc gtcgcgcaca ttcaacatcg tctcggcgga
    37141 ccggttgccg aacttgaatt gcgtgatcgg gggagattgt tgcaacaaga cgacatcgcc
    37201 caggcgaatt acctcgatct tcattttcaa cgagagttca ttcgccaggt ggatgttcgg
    37261 ctcaaccgtc agtttgacgc cggtgtcgcg aaactcgatc gatgtgacgg ttgaagtcgt
    37321 cggaacggcg ccggttgcgg cctgcccggg caacacgttc gtggtggaga gcaggatcgg
    37381 ttgcttgtca ccgatattga cctcggcctt cttgttgttg agcacgcgca ctttcggcgc
    37441 tgccagagtc ttggcgtcgg tgacctgctt gaagaaatcc aattgcacat tggtgggaag
    37501 cttgaacaga taactgtcct gtcccagact ggccaggttc cggtacgtga actgctgtgc
    37561 gatatccccg gcaatcgctc ccgtaaaccc gggcgggatc agagcagcgc cgatttgttt
    37621 gggataagac aatccatacg tctgatccac ggttcgattc acttcgagca cttccacgtc
    37681 gaagagcact tctgaatctt cccgatcgtt cgcctggatg atcttctccg ccagttccac
    37741 cttttccggc tgatcccgaa tcacgatggt attcagcgct tcattgccat gaatatgctt
    37801 cacatccagc atggacttca gcagcgccac catctccttc gctttggcgg atgacaggta
    37861 gaatgtccgg atcatgaggt cctgatactg ctcctgcttc tgcttggtat tggggctgat
    37921 gataaacagt gtggggccgg ccttctgagc aaacaaactg ttgctgttca agaccagcga
    37981 caaggcctca tcgaacggct tgtcctccaa cgagatggtg acgggatcgt tgcgcacatc
    38041 cttgtcgaac acaaaattga catgcgccgc cttccccaag gcctccacca cttccttcag
    38101 cccggcattg cggaattgca acgtcaccgg ctgcttctgc cggtcgtcgc gatccacggc
    38161 atgctgttcc gccgacagcc tggccacgcc ttcgagggct tctttataag tgggatcgag
    38221 ttccacggcc cgcatataca ccgccatggc ctcactcacc cggcccaatt gcgccagacg
    38281 ctcggcctca cgataccgct cgcgcgcctc cttgagtcgc aaggcttcgg tcaatccggc
    38341 ctcatgttcc ttgcttgtcg gctcgatcgt caaggcgcgt ttgaattctt cggcggcctg
    38401 atcgagctgg tgatccttca gcagctgccg gcctcgctca tcatgcgccg cggctgcgcg
    38461 ctcccgggct accgagtatt tgttttgcag tgacggctcg aacggatcat ccttgagcgc
    38521 ctgtcgatag gcgacactgg cctcttccca atttccagcc gcgagatgct gatcgccgcg
    38581 tttgacgtcg ggagagacaa acgttccaca gccggtgatc aggagaccac acacaacccc
    38641 ggccgtcgag atcagaaccc gacagcacca atggtctcta gtctcgacgt gtgtcgtgcg
    38701 gaaggatgaa cgcataggtc ggatcaacaa gatgataacc tctttggtat atcaaactga
    38761 cacagacgcg acaatcagtt tctcgctttg gacggcaagt gccatgccat cgtctcgcac
    38821 ctctttcggc agatctgtga cgaggcatta gtggtcagca catggagcca gcgggagggc
    38881 ctgtgaggcc ggacgatctg tccggcctca gagagagaga gagaaggatc accaataaga
    38941 tttcggcaga cactctggag cgcggttgag gagtctccgt ctgatcatgt cgacggtagc
    39001 ctagagcctc caatacttga ccgatcccgg caaactgtcc gggttcagca cactcttcac
    39061 gtcgaccacc acattgttcc gctgcttgcg caacggcttc agcagttccg agacgctcat
    39121 ctgcatgtac tcccgatggg caacagccaa cacgattccg tccagttgtt tgagctgatc
    39181 ccacggcgag aggcgcaggc catactcccc caccgcctct tccggttccg cgagcggatc
    39241 gtgcaccacg acctgaatgc catattcctt cagttcgttc acaatatccg gcacccggct
    39301 gttccgtaaa tcaggaacat tttccttgaa ggtcagcccc aggacgccga cgcgtaagtc
    39361 actgacgggt cgttccacct ggctcaacag tttcacggtc tgctcggcca catacttgcc
    39421 catgctgttg ttgatgcgcc gaccggccag aatcacttcc ggatgatacc cgacggattc
    39481 ggccttagcg gtcagatagt acggatcgac tccgatgcaa tggcctccga ccaagccggg
    39541 gctaaatttg aggaaattcc acttcgttcc cgccgcctcc aaaacggatc tggtatcgat
    39601 tcccaagcga tggaaaatca acgccagctc gttcatgagc gcgatattca gatctcgttg
    39661 cgtgttctcg atcaccttcg ccgcttcggc caccttgatg ctggacgccc gatgaattcc
    39721 ggccttcacc accatgccat acgtcttcgc cacgatctcc aaggactcct catcctgggc
    39781 agagaccacc ttgatgattc gttccagcgt atgttccttg tctcccggat tgatgcgctc
    39841 gggcgaatat ccgattttga aatcgatacc gctcttcatt ccggatgctt tttcgagtat
    39901 cggaaggcag tcttcctctg tcgcaccagg gtacactgtc gactcataca caacgatggc
    39961 ccccggagcg agattggagc caatcagttc cgacgccttt ttcaaggcag tcaaatcagg
    40021 ctgaagagcc tcgttaatgg gggtaggcac cgccacaata ataaaatcgg cggttttgag
    40081 atcactgggc tcggaggtgt accggacgcg gctggccttt aaatctttgg atgacacctc
    40141 tccggtccga tcaattcctt tgcgcagctc atcgaccttg gccttattga tatcgaaccc
    40201 gatcaccggt gcagatttac cgaacgccac cgcgatcggc aatcccacat accccaaccc
    40261 cacgactgca atctgccgct gcatcttctt cgccatgtca ctcccctcgt cagtcatcgc
    40321 cgccacgtca ccgatgtcgt aaaaaagaat ggcagaatat ctcccatccg tcaacgattt
    40381 cccataagcg tcgggacgtg cggaatgtag acacctcaca caacatctct ggccggaaat
    40441 tcatcaactg ctgttcgaat gggcaggctc aaccattttc tcgttgcctg accgatggac
    40501 ggtttcgcca ccttgccagc agaatttcgt catgtgcttg cctgcacgag cactattttg
    40561 accggtactg acctcttccc cccacagcat tcatgcctag gaagcataag aggccactga
    40621 gtcgccgtaa ccgagctccc atgaattgtg aaagcatttg caaggttatg tgagctattg
    40681 ctatggtagg agccctgaag gctacggcag gcaccgatct tgctcacttg atgaatgtag
    40741 gatacccgta ggacaggaag ccggttcagc cagatcctcc acactcacct catgcgggcc
    40801 gcacagtcga acggatggag tgacatgacc acaagaccaa ctgatacacc gtataccacc
    40861 aacaagagga caatcatgga tggcaattcg acgtctcagg cctctaagag gttggctaca
    40921 attgtggccg tgctgacttt ctgtgcagga tctgtcagcg ctcagcacag tcacgctgca
    40981 ggggtctgga ccaacgaacc ggcgggagcc aacgtggttg ttgattgcaa tttctccagc
    41041 acgccaggcg cttgcggtat cctggatgct tactcatcag ccgaggtggc ttcggactca
    41101 agcgcaccgt tatctccatc aggagtattg aagtccactc tccgcgcagg taattctgcc
    41161 ggcgggatgc agctaaactt tgcgacccca aaaccaaaca accgcgagat gttcatgggg
    41221 atcatatggc ggacaaaccc agaatttcag gggcgaccga atagcaataa actatttttc
    41281 cttcgcggcc cccaggccaa tggcgtgtgg atttttggaa atagtgccct cgtgaatggg
    41341 tcggctccat taatctttgg acacaacaca ggtggaggct tgctcgacaa cagccatgcc
    41401 tgttcagcgg atcttggact gctttgcttt ccaaacgtcg gaccaggatt actcacgcgt
    41461 ggtgtgtgga caaaaatcga agcctatgtc aagtccagca cgaccaatac gtcccgcgac
    41521 ggcatagtcc ggtggtggat caatggcgtg ctcgtgggga attacactaa tttaaactat
    41581 ggcggtaccg ctggaattaa cgaatgggtc tggtcggaaa cgtgggatgg tggcgtaaat
    41641 cctctcccaa cagtggattg gaaccatttt atcgatcacc tccacatcag tatccccaac
    41701 ggaggtagtg gtagtgacat tccaccgggc ccacctgcac aacctacact tcgaagtatt
    41761 acagtaccct gaagcttaca cattcccagc atgagtcgca aagggaattg ctgggacaat
    41821 gcctgtgtcg aaagtttctt cgggacactc aagcgcgagc tggcgtaggg cggtctgatc
    41881 cttggcctga ctcttccgat accacgacgc acgaccaaac tgggccaatc gacaggcccg
    41941 cgcacagctc acctcaaacg tctcgtgaaa ccgtaaactc ccccataaat cacgacactc
    42001 attgaggaga actttctggc actgacgttg accccattcc gagacagttc ttaaggagag
    42061 cagacgacgt tttcggtatc ctgttcttcc tgacgttgcc tgacgaactc gcttggggtc
    42121 aggtgcccaa gtgagctgtg gggacggcgg gtattatcgt ccacgcgcca ggcttcgatg
    42181 atcgcctggg cctcagccag tgaagcgaac tgatggacat tcaaacactc atcccgcagc
    42241 cgtccgttga atgaatcaat gaaggcattt tcgacgggtt tccccggtcg aatgaagtcg
    42301 agctgcacgc cgcggcgaga cgcccaatcc tcgagagcac gggactggaa ttccgtccca
    42361 tgatcgaccg tgatggagcg aggccgctgg ccctctccca ggacgcgatc caggacctga
    42421 ctcaccagcg ctccagacat ccgaaatccg gcctctagga ccggactaga gcgactccag
    42481 ttatccacca cggtcagaat ccgatagggc cgaccttcac tcaaggtatc atgcacgaaa
    42541 tccatactct aacgttccga tgggccgacc ggcacgggag ctggcccacg atgcagggcg
    42601 atgtgctttc gtcggcgcac ccgcatgcgc aactggagcc cctctaagcg ataccagcgc
    42661 cggactcgtt tccggttgac cgtccaactg acccaaaacc cgaaaagtag gccaggggga
    42721 gcttgaggaa atggccggcg gcgtggtctt ctttacccca aggaggaccc gacgatggca
    42781 cgacgacgag ggcatacgga agagcaaatt ctggccgcct tgcgccaggc cgagagtggc
    42841 ccgacggtcg cagaaatctg tcgtacggtg ggcattagcg agcaaccatt ctacgtgtgg
    42901 aaacgtaagt atgcggggct ggccctcagc gagctgcgcg agctccgaca gttgcgggaa
    42961 gaaaacacca agctgaaacg gttagtcgcg gatctctcgc tggatcgtca catgctgcag
    43021 gagattgtcc gaaaaaagct gtaaggcctc gggaccggcg ggcgctggct cgctgggcgc
    43081 agacgaccta ccaggtcagt gagcgccgag tctcgaggct gttgccgatg gcgcgtgcct
    43141 cattgcggta tcaggggcat cgtgatccgc aggaggcgtt gcgaatgcgg atgcgtgagt
    43201 tggctgcggc acgggtgcgg tttggggagc gacgcttgac ggtgttgttg cgacgcgagg
    43261 ggtggtcggt gaatgccaaa cgcatctacc ggctctattc ggaaaaaggg ctgaccgtgc
    43321 gcacgaaaca ccgcacgaaa gccgccggcc gagcccgtgt accgcagcca ggggccaccg
    43381 ccccgaacca gcggtggagc atggacttca tgagcgaacg ggtcgccgat ggtcggtcgt
    43441 ttcgaatcct gacggtcgtc gatcagttca cccgtgaatg cctctgcctg ctggccgatc
    43501 ggtcgctgac aggcgagaaa gtcgcacagg ccttagagct gctcgtgggg cagcgaggtg
    43561 tcccgcgctc gatcaccgtg gacaacggca gcgaatttgc cagtcgtgtc atggatgcct
    43621 gggcctatcg ccacgggtgt aatggcttgt caagcagcgg cccccgtatg tgtgcatggc
    43681 gtggtcggtg atgatggtgc cggcggcgag aggaacgtcg ccaatttctc cgcgggtgtc
    43741 aggccctgca aggccatgga gaagcggtcg tggttgtact gatgctccca ggtacgtagc
    43801 gcctgggtcg ctgacgcaaa cccgtcaaac gtcgtgcgac tccaaaattc ttcctcgtcg
    43861 atgcgatgac tgcgttccac cttcccattc tgttcgggcc ggcgcggctt gatatgccgg
    43921 agacgaatgc cagctgcttg cacggcgagg gcaaaggcca gtgggaattc tgtgccatta
    43981 tccacttgta ccttgcgaat ggaaaacggg aacacttgac ggatcgtggc cagaaactcg
    44041 agactggtgc cgtggtactt gcggggatac aagcgcaaca cgcgataccg cgtgcagtcg
    44101 tcgagagccg tgtactgaaa acatttctgg ccggcgacct tcacctcctt cacatcgacc
    44161 tgcacacact caccgggttg ctccttgctg aagagaatgg ggtgacgtgg acgccgcgga
    44221 tcggtgcgcc ggatgggagg atagccgagg tcccgacaga tccggcgaat cgtcgcgctg
    44281 gcgactcgaa tctgatgcac tcggttgagc cagatgcgcg tccgcatcgc cccaaactgc
    44341 aggtcgcggc gagcctgctc gatgagctgt acggtctctt cggacagccg tcggcggcgc
    44401 ttcaccggat ggcgcggcac caatccggcg ggcccactgg ccgcccaacg acggtgccac
    44461 gttctcaccg tcttgcgatc caatccgaat cgccgggcgg cgcctttgaa gccgtagtct
    44521 tgcgcatagc gtaagaccgc cagccggctg ttgatggtgt ggtgatgtcg tgtggcaatg
    44581 tgcaacgttc tcagttcctt cgtcgtgatc aatcgcatgg gatcccccct cgtcatagcg
    44641 tgtgactgta atccatgcag ggggccaact ctcaacaaga tatttcacac gttgcagcgc
    44701 cggatccggc gctggcgggc cacccacggg cccgagcggg aggtgatgtt cccgcaaatc
    44761 caccgcccgg gcgaatacgc gcagagcgat ttcacggcca tgggggcgct gggcatcacg
    44821 ctcaatggac agcccttcga tcacctcttc tatcacttcg tcctcccgta ctcgaactgg
    44881 gagaccggcg cgctctgtgt gtcagagagt ttcgaggcgc tgatcgccgg gtttcaagcg
    44941 gccgtctggg aactgggacg ggtgcccgcc ctccaccgga cggataattt gtcggccgcc
    45001 acccatgacc tgcgcgaagg cggccggacc ttcaacgagc ggtatcggca ggtgctggat
    45061 cactatgggg tacggccaga cgcgaatacg cccggccgcg gccatgagaa tggcgatgtg
    45121 gaacaagccc atcaccggtt caaacgggcg gtcgagcaag cgctgctgct ccggggacag
    45181 cgcgacttcg acagccaggc ccactatgcg cagtttctac gggcgattct cgctggacgg
    45241 aatcgaggac gtcaggaggc gcttcaggac gaactcccgc acatgcgtcc cctcccagcc
    45301 ctgcgtctgg acgatttccg cgtgctccga gtgatggtca atcgcttctc aatgattcgg
    45361 gtcggccaca acgtctattc cgtcgcctcg cggctgattg gggagacggt ggaggtgcgg
    45421 gtctatcccg aacgcctgga ggtgcggtat gccggcgagg gggtggcgac gatcgaccgg
    45481 ctgcgggggc gcgggcaggc cgccattgac tatcggcatc tgattgggtc gctggtgcgc
    45541 aagcctggcg ccttcgcccg gtatcgctat caagccacgt tgtttccgac cacgaccttc
    45601 cgccgggcct atgacgcgct cacggcctgc catgggagcc gggcagatgt cgaatacgta
    45661 cgcctgctcc agttggccgc ccgcacgagt gaatgcgagg tggagaccgt gttggggcgg
    45721 ctgctcgatg gcggcacacg ctgggatgcg atgcaggtga aggccctcgt gcagcccgac
    45781 gtgatcgccg tgccgccgtg tgcactcccg ccgcccaatc tctccgtcta tgacgcttgt
    45841 ctcgacgtgg tgggaggtgg ccgatgacaa cattgccggt gctcctccgt gcactctgct
    45901 tgcccacctt cgcccgtgag tatgcggtcg tggcggccca cgccgcccgc gaagggctgt
    45961 cgcatgaggc ctatttgcag acattggcga cacaggaggc cagtgaccgc gccgcccgtc
    46021 gggtggaacg cctgatcgtg gaggccaagt tgcctcgtgg taaaagtctc gcgacgttcg
    46081 acctcgcgcg actaccgccc aaagtccgca ccgccgtggc gacgctccga gagggggcgt
    46141 tcctggatca ggccaccaat gtgctcgtgt ttgtcaatcc cggaacgggc aagacccatc
    46201 tcgtctgtgg actcgggctg gagctggtcc ggcaacggcg ccccgtgctc tttgccccga
    46261 ccttccaact cgtccagcgc ttgctcgcgg ccaaacgcga tctccgcctc gcggccgaac
    46321 tgaaacggct cgatcgcttt gacgcgctca ttctcgatga cctcgggtat gtgcaacagg
    46381 accgcgaaga gatggaagtg ctcttcaccc tgctggccga acgctatgag cgccggtcgg
    46441 tgctgatcac cagcaatctc gtcttcagca agtgggacca gatcttcaaa gatcccatga
    46501 ccacggccgc cgccgtggat cgggtggtcc atcatgccgt gattctggaa ctcccgattc
    46561 cgagctaccg ggctcaagcc gcgaaagcga gaagtcaggc gtcatccggc gccgccacgc
    46621 cagacaggaa aagcatggga gacacgaccg tgaacgacga gcgtaccgac ggagcccgga
    46681 cggctcccta aaccggagga cggcccctgc acttctaatt gtcaccagcc ctcaaatcta
    46741 gttgacgcca gacaacgagc gcgtcgacga tcatgcgtgc ccaggtggac caatctgggg
    46801 aggcgtgcat ggtggatcag gaacggtggg cggagatccg acggttgttt gatgaggagc
    46861 gcgtgtccat ttcggagatt gggcggcggt tggacgtgga tcgcaagacg gtgcgccgca
    46921 gtctgcgaca gccgacctgg tgtaagctcc ccataaattg agacagtcat acggagaacg
    46981 ttctggcaga atagccatcc tagccaggag gttcccatgc gccaatcgag gttcaccgaa
    47041 acgcagatcg tcgcgatcct gaaagaggcc gacgccggtc gccccgtcaa tgagatctgg
    47101 cggcactacg gcatcagttc cgccacgtac tacaagtgga aggccaagta cggcgggctg
    47161 gaggcctccg atgtgaagcg gctcaaagat ctggagcacg aaaacagtcg gctcaaacgg
    47221 atgtatgccg acctgtcgtt ggagaatctg gccctgaagg atgtcatcgc aaaaaagctc
    47281 taaggcctgc tgaacggcgg gaggtggtca cgcatctcgt gaccatccac ggcctctcgg
    47341 ttcagcgggc ctgtcgggcg gtggaggtcg gccgggccac ctactatcgg ccgcgcctgg
    47401 actgggcgca gcgcgatgcg ccggtgatcg cggcgctgac ggccgtggtg gcggcgcacc
    47461 cgcgctgggg cttctggaaa tgctacgacc ggctacgact cgatgggcag ccgtggaatg
    47521 cgaaacgcgt ctggcgcttg aatctcccgc gacggacgaa gatacggctg ccgactcggc
    47581 tgcgccagcc gctctcggtg gtcccggcgc ccaacacggt gtgggccatc gactttatga
    47641 gtgacacgct gtatgccggg cggcggtttc ggacgctcaa tattctggat gaaggcgtgc
    47701 gcgaagggct cgcgattgaa gtggatacgt ccttgtcggc cgagcgggtg attcgtgtgt
    47761 tggagcaagt cgtggcctgg cgaggacacc cccaagccct gcgcctagat aatggccctg
    47821 aactcgtcgc tgaccgattt atgagctggt gtgtcagtaa tcatgccaaa ttgacccctg
    47881 tcatcatgtg aaatggaccc ctccc
//
LOCUS       DAORRQ010000031        47756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_45_length_47756_cov_29.952391, whole genome shotgun sequence.
ACCESSION   DAORRQ010000031 DAORRQ010000000
VERSION     DAORRQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 47756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47756
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45_length_47756_cov_29.952391"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PK224_11170"
     CDS             complement(<1..384)
                     /locus_tag="PK224_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16341.1"
                     /translation="MNRLSLLSVATISLVTSAVLPAMAGERAPVRPLFQVQGAVAHQA
                     QGLPQARGKRLIYPEHIRGVIHDFVKRELAGRAVDCQVAIGDPQQPIVVPSGTVDLQV
                     SAGRSDEPLGRRVFQIHLAVNGRFIK"
     gene            complement(418..1209)
                     /gene="flgG"
                     /locus_tag="PK224_11175"
     CDS             complement(418..1209)
                     /gene="flgG"
                     /locus_tag="PK224_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRB16342.1"
                     /translation="MIRAMWTAATGMTAQQLNVDTIANNLANVNTNAFKRSRAEFGDL
                     LYQIQRLPGTNASNVGVFPVGVQVGGGVRPITVAKEWVQGNMRQTGNDLDLAIDGAGF
                     FQVTRPDGTIMYTRNGSFKRDNVGNLVTGDGDQLNPVITIPSGALKIDVGQDGTVSVL
                     LPGVTQASQVGQIQLVRFDNPSGLVAMGGNLFLDSFASGPAQQGTGGFSTGFGTLQQG
                     FLESSNVNLAEEMVNMIIAQRSYEINSKTIQASDEMMQIANNLRR"
     gene            complement(1219..1986)
                     /gene="flgF"
                     /locus_tag="PK224_11180"
     CDS             complement(1219..1986)
                     /gene="flgF"
                     /locus_tag="PK224_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRB16343.1"
                     /translation="MNRAIYPILSGAVAQEQQLTVFANNLANVNTAGFKQDQQGFRGL
                     FARASSTGMGVMSGGLSSAISTRPAGPSERVFAEVHGVRTAFEPGRIRITGNPLDVAI
                     QNDGFFEVNTPDGVRYTRNGIFSLDSQRRLVTNLGHPVMGTKGEIKVPPGNIQINAEG
                     AIQVDGTPIGSIKVVEFPENAMPQKFAEGLFVGGKPTVSTRPQVQSGHIEESNVNSLS
                     EMVKMMQGMRSYESAQKLIQTMDRMTETAIQDLGRVQ"
     gene            complement(2265..3140)
                     /locus_tag="PK224_11185"
     CDS             complement(2265..3140)
                     /locus_tag="PK224_11185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRB16344.1"
                     /translation="MHSSRPELNTSSDRNGSDSTNRHGWPDDEAGTESLQSARLGVVA
                     CILFMAGGLSWTASVGLLTLSALTAYPVMISAGILLTLMVFGATLWTPRGPQSASRMH
                     SLSEESNGSGMNTYDSVTGLPTYRLFTSLLNQALVHAHKYGRPVAVLMIELDHFTPKT
                     DEQAQINLNLMYRVHAARVKSALRTTDTVARIAERTFVVLLDQVTGPEEVLAIARKMQ
                     LTISLPVTLDGHELFLTSRIGISLSSQDTMDIDALLDVATRAVATARAEGYALYGLPG
                     AMVSASVDATSTIAA"
     gene            complement(3300..4061)
                     /locus_tag="PK224_11190"
     CDS             complement(3300..4061)
                     /locus_tag="PK224_11190"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HRB16345.1"
                     /translation="MMDARKMSAVRNAPRRVIAEADRERVIQEFTHVVKAMAHRLAFR
                     LPAYMDAEDLMSVGIMGLMDAMDKYDPTREAKFKTYAEFRIRGAMLDEIRSMDWIPRS
                     VHERITLLQKTYSELLHRLGRPPTDQEVGKELKMSTEELDEFLTRSRGAVVISLDDLG
                     VQDADGHKIISVLTDSNQPDPLSVLVSERARHVLEAAIQDLPEKERLVLSLYYFEELT
                     MKEIGQALKVTESRVCQIHSKAILHLKARLEPVDV"
     gene            complement(4080..4946)
                     /locus_tag="PK224_11195"
     CDS             complement(4080..4946)
                     /locus_tag="PK224_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HRB16346.1"
                     /translation="MAMQPGILDPQMRALDDNVGPPRTQVITVTSGKGGVGKTNVVAN
                     TAIALAQTGKRVLVLDADLGLGNVDILLGLTPKYTLEHVLAKTCRLEDIALTGPHGIT
                     VLPASTGISQLTILTEAQQLILQDELERLASNMDVLLIDTGAGISSTVTYFAAAAQSI
                     IVVVSPEPTSMTDAYALMKVLLRQYRERRFHVLVNMVKSPRDAARIFRKLELAVSRFL
                     HISLDYLGAIPLDDYVPMAVTQQRAVIDLFPHAPSSRAFRELTEAVAQLTPPALPKGT
                     VQFLWQQLLRTH"
     gene            complement(4975..6258)
                     /gene="flhF"
                     /locus_tag="PK224_11200"
     CDS             complement(4975..6258)
                     /gene="flhF"
                     /locus_tag="PK224_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HRB16347.1"
                     /translation="MKVRTFHVKSMHEAIRSIKDTLGPDAVILSTKRVRSWDNGFGLL
                     GGSVLEVMAAIEDDAAVPEAAPLLGEDEQTRAAVTPAPMVPAEESRFQKTLQGAMETG
                     KEQSRPAVRQVPGPSRETRATPASTRHNGENQGATFQSFQRVYEDLLAQGVEHATAEA
                     CLRELRETLVEQGSAQRNSSLQLLRTVLLDRVSTAGPLLSAAGEQKIALFVGPSGVGK
                     TSTIAKLATHYGIVEQRSVALITMDTYRPAAVEHLRLYAEVLGIELSVAASCEEARAA
                     IAQAREAELILIDTTGFNPYEPITTQRWRGLLNGAYPMEVHLVLAAGTRVPDLVVSTS
                     QCVDVPGLRLLFTKLDETAGYGGIFEATHRTGIPLSYWGTGQRVPDDLVQAQVDRLGD
                     LLLGGQVRTPAGGTTQSWDRQTTSAVVPGIKTYAR"
     gene            complement(6255..8363)
                     /gene="flhA"
                     /locus_tag="PK224_11205"
     CDS             complement(6255..8363)
                     /gene="flhA"
                     /locus_tag="PK224_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRB16348.1"
                     /translation="MANENTSVSTTSLVKHPDIMMSVGVVGILMVMLIPLPRFFLDLL
                     LAFNITLSIIILLVGMQVRRPLEFSVFPSILLMVTLLRLALNIASTRLILLHGNEGAA
                     AAGEVIRAFGTFVVGGNYTVGLVVFTILVIINFVVITKGAGRVAEVAARFTLDAMPGK
                     QMSIDADLNAGLINDKEARQRRKEIAQEADFYGAMDGSSKFVRGDAVAAVIITLVNIL
                     GGLTIGVLQQGMTLAAAAQTYTLLTVGEGLVAQVPALIVSTAAGIVITRAASEVNLGF
                     EISRQVLISPKAIGTASGILITLGLVPGLPHLAFLALGGLTAWMAYQINEQQKTAEAV
                     PTPASPQVKAEEAGTQVVPLDLMEVQVGYGLISLVDGGQGGALLDRIKGLRRQFAEQM
                     GFVLPPIHIRDNLQLRPNEYAALLKGVELAKSEVMPAHVLAIDPGTAQRGAIHGLPTK
                     EPAFGLPAMWVPEQQREQAQMAGYTVVDPGSAIATHLSELIKRHAHELLGRQEVQGLL
                     DQLGKNHPKLVEEVIPNLIPLGTLVRVLSHLLREGVPIRDLRTILETIADHAATTKDA
                     DILTEVARQALGRTITKQYLAPDGSLPIIGLDPRLDRTLADQANAAGQGTQWVPDPLV
                     AQKLLTALKAAAERMVGRGHQPVILCSPSLRRHLRKLTDRILHSVPILGLNEVESVTR
                     LQAVETVRLDLEVGETRSLA"
     gene            complement(8386..9468)
                     /gene="flhB"
                     /locus_tag="PK224_11210"
     CDS             complement(8386..9468)
                     /gene="flhB"
                     /locus_tag="PK224_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HRB16349.1"
                     /translation="MADSGQNRTERATPKRKADARAKGQVALSRDAAMAVGLLGSLGA
                     LYWMTPGILNKLRESLQLWLSKSMEESTQRTLSLDHLHLILRQIGIDVFIMLGPVIAG
                     IAVVGVGANLMQTGFLWKRDGLQLEFSRISPMGGFSRLFSVRSFSELVKSWLKIFAIG
                     GVGYLTIKQDMLLFSPLTQFGMETLLPTVGWATFKAALMMGGAALVIGGLDYGFQRFE
                     WERDLRMSRDEIKEESRAAEGDPGLKAKIRSTQREMSRKRMMAEVPKADVIITNPTHL
                     AVALKYDSKAMGAPIVVAKGAGFVAEKIREIGRQHGVMIVENKLVARTLFKLVEVGRE
                     VPEDLYRAVAEILAFVYRVRGKLPSA"
     gene            complement(9461..10249)
                     /gene="fliR"
                     /locus_tag="PK224_11215"
     CDS             complement(9461..10249)
                     /gene="fliR"
                     /locus_tag="PK224_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRB16350.1"
                     /translation="MSAAQTLHLALPQFQAFSILLVRIAGIVSVFPILNTRTIPMPVK
                     TGLVATLGLVLAPIIHLPSLPTDPALVIAGIGSEFLIGLTIGLAVRLLFSGFQVAGDM
                     IGTQMGFSAIQMFDPMSHQNTPIIAQFQLTLGSLVFLSLNAHLLVVHAIGMSYEYVPP
                     FGASLSDGIAQDIIHLAQNMLGVALKLAAPVFATLLIVNTVLAILGRAVPQMNVFVTS
                     FPITIGAGFLAMSLAMPFTLLLFEKEFETLVETILGLLKALGHG"
     gene            complement(10266..10535)
                     /gene="fliQ"
                     /locus_tag="PK224_11220"
     CDS             complement(10266..10535)
                     /gene="fliQ"
                     /locus_tag="PK224_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HRB16351.1"
                     /translation="MTIETVTEIGRQAIETTILVSAPILGLSLIVGLLVSTFQAMTQI
                     NEATLTFVPKVLAVFAATLLFLPWMMGVLIAFMTHIITSIPSLIH"
     gene            complement(10547..11347)
                     /gene="fliP"
                     /locus_tag="PK224_11225"
     CDS             complement(10547..11347)
                     /gene="fliP"
                     /locus_tag="PK224_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248836.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HRB16352.1"
                     /translation="MHTASLLIPPRQASAAQRSGWWAGLLISLILLTCLFSVSFAYAE
                     GPSLTIDLGQGGGKQTAVVLQILALFTVLSLAPALFIMVTSFTRMVIVLSFLRQAMGT
                     QQVPPNQVLISLALFLTMFVMAPVGQAVYKDALQPLLAEQIGYEDAWNRGIQPIRAFM
                     LKQMRDKDLELFMELSHAPKATQVEQVPIHVIIPAFVLSELRIAFQIGFLLYIPFLIV
                     DMVVASVLMSMGMMLLPPVMISLPFKLILFVLADGWYLVVGSMVRSFE"
     gene            complement(11334..11702)
                     /locus_tag="PK224_11230"
     CDS             complement(11334..11702)
                     /locus_tag="PK224_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRB16353.1"
                     /translation="MEIWESVVRMVSALGVVLALILGLLAVARSAIGRRFLPVNGAPL
                     VKILGSGPLGSRKHVMVVAVAGEVLILGTTATDLVPLGKIADPEVAKRLIAEASALHS
                     GAPTGIPGSGNLEETARAHS"
     gene            complement(11760..12134)
                     /gene="fliN"
                     /locus_tag="PK224_11235"
     CDS             complement(11760..12134)
                     /gene="fliN"
                     /locus_tag="PK224_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRB16354.1"
                     /translation="MGNGDTTAPSDASNAGAGDINVQPTSFPPVDNQNAVPASKNIEF
                     VLDIPLQVTVQIGSTRMLIRELLQLGQGSVIELNKLAGEPMEVLVNNKLVARGEVVVV
                     NEKFGIRLTDVVSPNQRIQQLG"
     gene            complement(12164..13135)
                     /gene="fliM"
                     /locus_tag="PK224_11240"
     CDS             complement(12164..13135)
                     /gene="fliM"
                     /locus_tag="PK224_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HRB16355.1"
                     /translation="MDKILSQEEIDALMGGVMSGEVETASKEEVDPGGIKAYSLTSQE
                     RIIRGRMPTMEMINDRFTRQQSISWSAVLREKIEFSVLGAQIMKFGDFIHKIPVPSSF
                     NLFAMEPLRGNGLYVMDAMLVYLLVDFFFGGKVQTHVKPEGREFTTIQLRLIKKLVQQ
                     ALADLEKAWQAVMPVKIGYLRSESNPQFAMVVTTSEIVVVVTLQVHLGDISREMFIAY
                     PYSMLEPIKEKLYSGLFADNVEQDSSWGSRFKDSLQECDVAMTVQLGTARINVQDLLN
                     FNPGDVLMLDQHPGDPMLCLVEGDPKFQGQPGIFKGNHACRVTKILG"
     gene            complement(13160..13702)
                     /locus_tag="PK224_11245"
     CDS             complement(13160..13702)
                     /locus_tag="PK224_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRB16356.1"
                     /translation="MSEAAEEKAPVASAAPAGMPMKMVIILLGGTLIVALGGAFMMFK
                     MMSGSSEKPAAEGAAETSAAHGEAAAKEGGHAAGGETGSIADLDPFIVNLADSPEVRY
                     LKVTIKLEMENLGAVEDVKVRTPQIRDAILVLLSSLDSVTARSPQGKYKLREDVTQRI
                     NGLLPKKSVKMAYFTDFVIQ"
     gene            complement(14035..15258)
                     /gene="flgE"
                     /locus_tag="PK224_11250"
     CDS             complement(14035..15258)
                     /gene="flgE"
                     /locus_tag="PK224_11250"
                     /inference="COORDINATES: protein motif:HMM:NF004238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HRB16357.1"
                     /translation="MGILTSMFTAVTGLSSYGNAMGVIGNNIANVGTAGFKSSRATFS
                     DLISSSLAGGSGGDQVGLGVYMNDVQTNFTQGSITTTGNTFDLAIDGNGFYLLRDSAG
                     ANFYSRAGQFKVDNLGQVVDSSGALLQGYQADTNGNITSTIGSITLSSSAVAPQTTTT
                     AAILGNLNANATAPTTAFSVTDTTSYNFSTGMTFYDSLGNSHQMQLYFRKTAANTWGV
                     YSQIDGGAAAAQTNMTFNASGALTAGGTQTVTAALTNGATTPQAVTVSLAAMTQFGSA
                     SGIISQTQDGFTSGTLDRISVDKQGHVVAQFTNGQTRPLAQVVLNRFLNPDGLVSNGD
                     NHFLETIDSGAPLSGAPTTNGLGRILSGTIEQSNVDLGKEFVDMIITQRAFQANSRAI
                     TTSDEMLQELVNLKR"
     gene            complement(15333..15623)
                     /locus_tag="PK224_11255"
     CDS             complement(15333..15623)
                     /locus_tag="PK224_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HRB16358.1"
                     /translation="MDISKVITQATPPDSSTPTTDGPKPLGKDDFLKLLVTQLQHQDP
                     LKPMENEDFVAQTAQFSQLEQMSKLVSLMEKSVTLQENAQNKTATTGTTAVA"
     gene            complement(15635..16894)
                     /locus_tag="PK224_11260"
     CDS             complement(15635..16894)
                     /locus_tag="PK224_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRB16359.1"
                     /translation="MKADCVSDTEVESPNAEDNTEQAEDQSTQRQEILVAALLLPTPA
                     VAQTEVQISGETDSELDPSADGAGKVAPTAATGPHETPSAQLAALDPSVQTSEEALVG
                     QAATAQAQKSDIKESAEKEQPGEGMIDMSAFPHPDSHGEAREVSADRTDAAVTASTID
                     PEKVTTVAAAVTTRTAEAGGMKQADGDRVDRPTFLEDLAQDSPVSTEQGSANAGKFLE
                     NESEPFSDSSADADRSMPNQAMSNGPIDRPLFLDRMSGLNQPAPPLSESAVGRNEAGQ
                     PAAVLRAAEAERMSELRGASPVSQSVMLDLDPLDMGPLRVRVMMSDQTVHAHIRTEHG
                     ELGQGLLQQGQSLESSLRTTGLEMGMLRVTVDQQQGRNDNAWMFQQQHQDRPTTSSGQ
                     RSTTGEEERSARGETGDCTNERVSIFA"
     gene            complement(17408..17977)
                     /locus_tag="PK224_11265"
     CDS             complement(17408..17977)
                     /locus_tag="PK224_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16360.1"
                     /translation="MALLGGMGTAVVADEGAPAPAPAAAKEEPKADAIKKEVQGPAIS
                     TPREIFQMLEQRKRALDKREAALRGSEMHLLELKAELEQIVTRHEQAVEAEKKRRQAA
                     QNKATSEAEKPKPTAKPGGAANVNQTQLAKIYEAMPAEEAAARLERMPDRKALEVLRL
                     IKGKSAGAILAEVKPERAARLTEQLLTAP"
     gene            complement(18043..18483)
                     /locus_tag="PK224_11270"
     CDS             complement(18043..18483)
                     /locus_tag="PK224_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HRB16361.1"
                     /translation="MNVTVLRTYAIQLEEVAKLELAELASALQQTVDRMTMLDSRARA
                     DADQYLLQVRQGSTVDQVYGHLDAMDQAIAARKSMEQMQRDQQVKMEDKRGELLEAMQ
                     YRKKLDLLDARAALERRRRRERQDQQLLDERAWRRSPLQQGERA"
     gene            complement(18480..19787)
                     /locus_tag="PK224_11275"
     CDS             complement(18480..19787)
                     /locus_tag="PK224_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HRB16362.1"
                     /translation="MKLSERLEQVEPLSVTGRVAQAVGIVIEASGPFTSVGEVCEITR
                     EGGHGAVMAEVVGFREDRVLLMPLGEMQGIGPGSRVVMKGHVASVSVGPQMLGRIFDG
                     LGHPLDGLPPVRTDIQVPLYAPPLNPLHRARITQPVDLGIRAINALLTCGQGQKIGVF
                     AGSGVGKSVLLGMISRYTQADVRVIALVGERGREVKEFLERDLTPEARARSIVVVATS
                     DQPPLVRIRGALVATAIAEYFRDCGKHVLLMMDSLSRLAYSQREVGLAIGEPPTTKGY
                     TPSVFAVLPKLLERVGPAQSGGSITGLYTVLVDGDDLNDPVADAVRSILDGHIVLSRE
                     LAARNHFPAIDILQSTSRVMRDIVTPAHYAAARLVLERTALYRRSEDLITLGAYKPGT
                     SKELDKAVSAHEGITAFLRQETKQSVGLSQSIDALLALAGTIQ"
     gene            complement(19777..20826)
                     /locus_tag="PK224_11280"
     CDS             complement(19777..20826)
                     /locus_tag="PK224_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB16363.1"
                     /translation="MRTVDVGSRLPQMSPADNEKELAPRAILIVDDEPSIGFLLTEML
                     QSTGHPILTAGSVSEALTHLKSRPIAVLIVDVQLAGTDGIAFLQQALEVDSRLLSIVM
                     TGHGNIELAVRAMKSGAADFLTKPFEVELVRQSVSRLLELYRLRQENTVLTRTLIRSG
                     NIQLRTVPLADFSRGNRPFGTDDVTEFERGVTEGEKRVTERVAALRQREQALVASLMA
                     QLEGSWRNLHDTVEEEVASLSFMIAQKVLRELVTEKREVIVTQVRTALAHLHESGLVR
                     IRVHPSDLPLLEASRAALSQTPHGLLTLKFETDPGISPGGCLVQAQSLLIDATLDTQL
                     LRLGEALRKRDTGEA"
     gene            complement(20827..21822)
                     /gene="fliG"
                     /locus_tag="PK224_11285"
     CDS             complement(20827..21822)
                     /gene="fliG"
                     /locus_tag="PK224_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRB16364.1"
                     /translation="MNKELSGAQKAAILLRAIGEEAAAAVMKTLDPKDIRKLGSFMKE
                     TANITKQEEDSVIAEFEQASSSGEVQFEGREFMEAILKKALGPEKAARMMESLNTKTY
                     PGIDALKWVDPRTVAQILKIEHPQTIAVCLGQMEAEQASAVLAFLPVHLHADVSLRLA
                     TMQEVQPEVLSELSDSLQEILSASMGMSSMSVGGAELMADILTRVDKNTEGAIMAKIT
                     ERDQALAESIRALMFVFDDLVELDSRGMQELMKEISKEDLPVALRGATPEIKEKFLKN
                     MSSRAAEMLKEDMETRGPVKVSDVEKAQQNILKVCRKLEEEGRIVIGGGGGEELL"
     gene            complement(21834..23390)
                     /gene="fliF"
                     /locus_tag="PK224_11290"
     CDS             complement(21834..23390)
                     /gene="fliF"
                     /locus_tag="PK224_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRB16365.1"
                     /translation="MFSKFSQFTINQRFIILLALAGSIAGLVAVTLWTQQPDMQVLFA
                     NLAVDDASGIIDKLKDAKVPYETANGGTTVLVPSAQVHDLRLEMAGQGLPHGGGVGYE
                     IFDRTTMGMSDFVQKLNYRRALQGELARTITQMPEVERARVHLAIPERRLFATEQDRA
                     RASVVVSLRASQTLSKAQIQGVVHLVSSSVEGLQARDVTVVDGHGNLLSNTSSDESAG
                     LSGTQMEYQRTLEKDIETRIQTMLERIVGVNKAVVRVSSVLDFRKIETTEERYDPNGQ
                     VVRSEQRGQEKSSGVNGTSGGVPGVESNVPGGTEAEGGQTSSNNNQTKNETVNYEISR
                     TVSRIVEPTGTIKKLSVAVLVDGTYEGGGKAGEGGADQPKKYVPRSEEEMKRIEEIVK
                     KAMGYSTERQDQVEVVSIQFGLGAEEPAGGVEATPDASKAWMPYVRYAVGGVLFFLIF
                     FMVVRPLMVMLVQSAPAAGGGETPALPASVGQVEAAISGKQPGQILDMAKNNPANTAV
                     VVKQWLKSNA"
     gene            complement(23440..23751)
                     /gene="fliE"
                     /locus_tag="PK224_11295"
     CDS             complement(23440..23751)
                     /gene="fliE"
                     /locus_tag="PK224_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRB16366.1"
                     /translation="MSDLRIAGANGFRPIEMPEIHEAGPGGEAGAANFMGSLKEAIGH
                     INDAQTGASQAVDALVTGQSTNIHQTMVALQQADVSFQLMMQVRNKLVTAYEEIQRMQ
                     I"
     gene            complement(23754..24203)
                     /gene="flgC"
                     /locus_tag="PK224_11300"
     CDS             complement(23754..24203)
                     /gene="flgC"
                     /locus_tag="PK224_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRB16367.1"
                     /translation="MELTDSLAVSVSALDAHRHRLNVIASNLANAQSTKTSSGGPYRR
                     RDVVFQAAPVSSAFQKAFKQVTSGPGRQALDGVKVARVIEDKKPGQTVYDPRHPDADK
                     KGFVTMPNVNVMEEMVNMIGASRAYEANVQAINATRTMWNRALEIGR"
     gene            complement(24264..24671)
                     /gene="flgB"
                     /locus_tag="PK224_11305"
     CDS             complement(24264..24671)
                     /gene="flgB"
                     /locus_tag="PK224_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRB16368.1"
                     /translation="MTIFDRTMQLLSRSLDLRGARQQVIAANIANEETPKYRAKDLNF
                     GQALASAQQGKLPISLVSTNAQHFGPQGTGYQRVHGQLEDVPAGDLPLDANSVNIELE
                     MAKMSDNAQQYNTAATIIGMKFKGLLGAIREGR"
     gene            complement(24841..26259)
                     /locus_tag="PK224_11310"
     CDS             complement(24841..26259)
                     /locus_tag="PK224_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB16369.1"
                     /translation="MSVQQDLQGPSATSEEARMVLIVDDEPSMRTALSETVRRLGYQV
                     RGAIDGVDAIEQIERVKPWLVVTDLKMPRLTGLELVKAIKQKSPQTFIVLMTAYGTVE
                     TAVEAMKYGANDYILKPFSTDLLERVILNLQATTAPDEQEGPATLEARAILTQDPGMI
                     RLLTTLEGVAASQATVLISGESGTGKELLARYIHARSPRAHRPFVALNCAALPDSLLE
                     SELFGHERGAFTGAIQKKLGKFEMAHTGTLFLDEISEMNLGLQSKLLRVLQEREVDRI
                     GGREPVPVNIRVIATTNRSLYHEVTQGRFREDLFYRLNVFPVTVPPLRDRSGDIPLLA
                     RHFLRSSAQRNGLTMPTLSERAIADLQQRPWKGNVRELENVMERAILVATGGAVDVDH
                     LMPGDGTVPVREVEVGDAIVTPSAHGSLWEMERDLIFKTLARVKENRTHAAKELGISI
                     RTLRNKLREYRDMGYQVEAEKS"
     gene            complement(26256..27545)
                     /locus_tag="PK224_11315"
     CDS             complement(26256..27545)
                     /locus_tag="PK224_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16370.1"
                     /translation="MTARDAEASNNDLLTRAFHDFDQAATVLQQSYDALTTRLQQMDL
                     ELAQTNASLREHLRETEEMRAHVTAVLESLDTGVIVADSQDSVVRCNHATERLLGVPQ
                     ARLRGRRATEVLEEIRKDHGEYPLVLPTGVTIALSQSDLTDETGSLIGKLVLIHDVTH
                     IRQLEDRLQRRNRLEAMGQMVGNIAHEIRNPLGSVELFASMLRKDLRDQPHLRTYAEH
                     ISVAVQAMDRLLSNLLVYTRPDCSRLAWQDTESLIREVLTLASHAMSPASIVVRCEVD
                     PRVPKIWCDGGKIKQVLLNLVLNAVQAMPEGGSLILSARMAPEEISELPAIQLTVSDT
                     GTGIPLELQSRVFDPFFTTKDHGTGLGLAIVHALVEAHHGRIDVESRPGQGTSFVMRL
                     PQGSIQQPTLSARNAALVGRSVQTRPIENATEEETYE"
     gene            complement(27542..29599)
                     /locus_tag="PK224_11320"
     CDS             complement(27542..29599)
                     /locus_tag="PK224_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16371.1"
                     /translation="MRQQLPAMMPEPSELVRGALPDPGPRLERIPESPEWGEFERGVA
                     LYNQGQYLEARLHLTNMLREHRDIPLRSSIQAFLTESSLKSSAQELRPLEIIDQYKAL
                     IREDPQSTNARRAAWRIGDVYRAEGWYQEAQIAYQHALSLSERDSYDANRAMLGLGYV
                     FRGIKSWKDSVQTFDHVLKRTTDPTLLVSASLGQAHSLYRMGRIKDADALYESISSRW
                     PGALRADPFALLRYADTAGEAHRGPVMREQLLHFYNLYPSRPENPFVLMHLADSYKEA
                     GRWEDASIFYAALLSQYPDAQVVPTAKLRYAEVQEHLAPEGEEVNLRHTIAAHLANVP
                     LKPGEMLSPRQLFESSAKQYEDSPVGSEALFHLGEALERAGKREDALKAYEQVVQRAG
                     KFENDPWPEKGGAQLVRFLRPRLEAALKADDDFELINLFHRNGPFADRLYAGTELLLK
                     VAEAHRRLGFPIESARLFQSLIRDSKAEAFHEVALMGLGDSYLEQKDMRAARAVFERY
                     RLQFPVGRFAGQALLGILTSFEGEGNTPGLVKLGKQWLIHNPRHPDRAFVQLKVADAL
                     RQVKRDAEAAPLYESVLKAGMDLSALDRLRYADVLARLNRQEPALALYKQALIAGLEP
                     EQEAWAQFQIVQLARGVKREEFARNGLRALNENSDSLVRRMAAVLQTELPEPTSAAGG
                     KKR"
     gene            complement(29896..31311)
                     /locus_tag="PK224_11325"
     CDS             complement(29896..31311)
                     /locus_tag="PK224_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB16372.1"
                     /translation="MSQSHILVIDDDPAVRQILAETLAGEGHQVTVMSSGLDGVEAVK
                     DQPVHVVLTDLQMPGIDGLETIDRISKIDSKIIAIVMTGYGTVDYAVRAMKAGAFDFI
                     TKPFEPDTVAVVVRKALDVYKLKQENHLLRKAVRDQYRLEHLVGTSAPMRMVLDFVEK
                     VADSDSTVLIEGESGTGKELIARMLHFNSMRRERPLVPVNCGAIPETLLESELFGHEK
                     GAFTGAAHTRLGRFELAHGGTIFLDEVGEMSLPLQVKLLRVLQERCFERVGGTRTINV
                     DVRIIAATNQDLAQAVQERRFRQDLYYRLHVIPIHIPPLRERRSDIPLLVNHFIAQFN
                     QLRRTEILGMEPDALARMMEEEWPGNIRELENMIERLCVLKKRGMITMGDLPERPVKM
                     NAAKGVESPEQFIRFSEDGINLTKELEHYENRLIGEALRKANGITSRAAQLLQVNRTT
                     LVEKLKRKGFDPKTHGYSVQN"
     gene            31620..31695
                     /locus_tag="PK224_11330"
     tRNA            31620..31695
                     /locus_tag="PK224_11330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31653..31655,aa:Thr,seq:tgt)
     gene            32255..32632
                     /locus_tag="PK224_11335"
     CDS             32255..32632
                     /locus_tag="PK224_11335"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB16373.1"
                     /translation="MKNRYSQRVSVDCSVMFAGENVVAEGRILDLSLPGCLMESSKKM
                     STGEYIQLRLFLSDHQAPLHVALAAIRWAEGSKVGIEFIRTSQSEQHRLEQFVRRSSG
                     CDASSTLSDRGVLAGTAWNEKQS"
     gene            32670..33011
                     /locus_tag="PK224_11340"
     CDS             32670..33011
                     /locus_tag="PK224_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB16374.1"
                     /translation="MPKHFKLRTYQRAMICGTAYYLSEDFLGKGTVWDMSSGGWRIQG
                     DHQVKIGMLLTLRMELPEEKMPLEIEHAVVQWVSGEDFGVRIKKIRRSAAIKLERLMG
                     HHLCILPPVEH"
     gene            complement(33008..34423)
                     /locus_tag="PK224_11345"
     CDS             complement(33008..34423)
                     /locus_tag="PK224_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB16375.1"
                     /translation="MEQERILVVDDDEGLLHLLRMRLSALGFSVTPCTGGRDALTAAR
                     QETFDIAITDLRLRAEDGLALTEELMQIQPGLPVIILTAHGSIPNAVEAMQRGAFGYL
                     TKPFDDKELKATLDKALIQLRMTREIQRLKSLVKELYGLENVIARSPAMQRLFQQVAQ
                     IADSDVTILLTGETGTGKEVLARVLHANSRRSKGPFVALNCAAISETLFESELFGHIR
                     GAFTNAVAAKRGLFQSANGGTLFLDEIAEMSLPMQVKLLRAVQEREVREVGADYATKV
                     DVRIITATNKDLAESVKAGTFRHDLYYRVSVVPLAIPPLRERKDDIPLLAQHFLKQSA
                     KRSNKDVRGFTPAAMHRLMVYPWPGNVRELENAVEKAVVMSRQDMVLPELLPTAGVAS
                     DIGLKPLTEAKEEFERSYLRNVLQMTGGNISRAAQFAGRYRADFYKMLKKYGLHPSML
                     KERADLDLTDLEEATEEVKDV"
     gene            complement(34432..35232)
                     /locus_tag="PK224_11350"
     CDS             complement(34432..35232)
                     /locus_tag="PK224_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16376.1"
                     /translation="MSDKTGDTGIVWHLRNMAVLLGLGLLSACSTVSPPPGQTPYFYG
                     DGSDGKALQAIIRKEIQLVPKCASKNTCDHVFFTRALASLYESRESASQYFEKVIAVA
                     PKSQLAASSRLWLQLLQSEEVPADQSWIRSVWNAPATAHTQRLLAQAIERTVHDLLDR
                     ELTIQQLRALQDSEAQSLEALQRELQDQERKAELFNSRREHPRMSVEPATLQSLQRQV
                     SDRDRKIEELTSQLEALKRIDQEMRGKTRPIKPPSNVFSPQQPESSKP"
     gene            complement(35202..36674)
                     /locus_tag="PK224_11355"
     CDS             complement(35202..36674)
                     /locus_tag="PK224_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRB16377.1"
                     /translation="MRLSIFWRLILTYVVIIAVMTAVNIYALLQIRTLAGLNTEVGSH
                     HHPEIESAKRLLSSFYEQVQSEKKYVAVPAATFLEHFDAESKDFQLILQGLLMRDTSE
                     PEIRLVREVERIQQAHLALFHTQLAEGVGRPAESSAAYDERRDALAGKMASTLQQYIG
                     LHEARIAVGVNESHASSVHAEAVTRQLVLVALMFGLGMAAVASYNILRPLRRLQSTIQ
                     EMGQGNFRATMDGPAPRELRELGDTVKWMGARLQQLDDIKSEFLAHVSHELRTPMASI
                     QEGTHLLLDEIPGPLTQDQRTTLRIMSDSSRRLMYLISTILDLSKMESGMMEYRFAPT
                     DLRRVADISVNKIRLLADAKQVRFLVEAPVERHWVRADSARIEQVLDNLLSNALKYSP
                     EGAAVKLHMEPRRDAGLLFVDVTDCGPGIPTEEVPHIFERFYQGRTKTRHVSVGSGLG
                     LALAKKVVEAHGGRIWVESKVGKGTTVRFILCLTKPGILA"
     gene            complement(36821..37639)
                     /locus_tag="PK224_11360"
     CDS             complement(36821..37639)
                     /locus_tag="PK224_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16378.1"
                     /translation="MLTVVTSTLHLYREALHATSRSLVRGWLAIPAVIVFTLLMLLAH
                     QIARPLGMLGGFLLGAVNACLIGAILSLIEQAIKGMRAITLGDVIESMGHYFWDVISV
                     GFVLWIPTLLLDTSLRANPDSQFLVTACLLLVFILLNPAPEVIYLTRHDSPLDVLKRS
                     YDFVLDNWIEWFLPMALVLAPLGLSLFFKLSSQLGRGAGLDFIQLVFVPFTVLSAWLT
                     DFGISAELTGMIALLLTPPLMVGMLIFRGHLFVALSATSRRQRLYQSQFHDSAR"
     gene            37870..40464
                     /gene="clpB"
                     /locus_tag="PK224_11365"
     CDS             37870..40464
                     /gene="clpB"
                     /locus_tag="PK224_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRB16379.1"
                     /translation="MDMNRMTIKLQEALQSASALAMRRGHQGIDVEHLLLALMEQEGG
                     IAGSLLEQAGVSPAAVRQAAEQALTKVAQVHGPGAAPGQIHLTPRLGSLLTKAEDRMK
                     ELRDEFVSVEHIILAMVEEGGIFRRLNLTHERLLTALQQVRGNQRVTSQDPEGTYQAL
                     EKYGRDLTKLAGQGKLDPVIGRDDEIRRTIQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKGDVPEGLKQKRVVVLDMGALVAGAKFRGEFEERLKAVLKEIQSAQGQVLLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGEMHLIGATTLDEYRKHIEKDAALERRFQT
                     VLVDQPTVEDTISILRGLKERYEVHHGVRIKDAALVAAAKLSNRYIGDRFLPDKAIDL
                     VDESAARLRTEIDSLPAELDEVSRKVLQLEIEREALKKETDAGSKARLQSIEKELTEK
                     NRDLQALKTRWESEKNSVSKLRKIRQQIEEVKQKIEQFERAYDLNKVAELRYGELPRL
                     ERELELEQQHLGKKQNESRLLKEEVDEEDIAAVVSRWTGIPVTRLVEGEMEKLLKLDE
                     LLHRRVVGQEQAVTAVADAVLRARSGIKDPNRPIGSFLFLGPTGVGKTELARALSVTL
                     FDDESNLIRIDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRHPFSVILFDEIE
                     KAHHDVFNILLQVLDDGRLTDSQGRTVDFKNTVLIMTSNIGSQHILEAQQAGASYETM
                     KAQVTGELRAHFRPEFLNRVDEIVVFHALGNEHLTKIVDIQLERLRARLVERRITLTV
                     TPDALQNLGRRGYDPVYGARPLKRLIQQEIETPMARQLIKGELRDGDTAVVDLKDGHI
                     AIVPTVAP"
     gene            complement(40509..40934)
                     /locus_tag="PK224_11370"
     CDS             complement(40509..40934)
                     /locus_tag="PK224_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16380.1"
                     /translation="MDIEVGSNLYRNSNGIIDIEGVPQFEVAIKEPARALLVNFALFD
                     DVGRMMAKVVDSNLTFNERRAYQLAKSPTSVSLKHEESGTVVFTLELKEGNRVVFSRG
                     SFHTIKGHRLDVSQTEWRIDKKRFSGKDTDTKGGAVFIG"
     gene            complement(40976..41206)
                     /locus_tag="PK224_11375"
     CDS             complement(40976..41206)
                     /locus_tag="PK224_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16381.1"
                     /translation="MKESPVIHTARVFAWWFIVGATMALAVILLQGGIREALQAQGSV
                     WELKLVELLTTILGGGLLGGCIALILDRIKKA"
     gene            complement(41203..42732)
                     /locus_tag="PK224_11380"
     CDS             complement(41203..42732)
                     /locus_tag="PK224_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRB16382.1"
                     /translation="MLANVLSAAIVGVDAHLVDVEVDISSGLPQFSIVGLPDATVRES
                     RDRVRAALKNSGFHFPVKKVTVNLAPANIKKEGAGLDLAIALGILAAEDIIPPEAVKD
                     FVFVGELSLDGRLKPIPGALSIGAVCRRRHPVLVSAENAGEAALVEGTEVFPIHTLPQ
                     AVEFLRGALTISKVSVSQDGSASSGLPEDEDFADVKGQAHAKRAIEVAAAGGHNLLMM
                     GPPGAGKTMLARRLPGILPLLAQEEALETSRIHSVVGQLSKEQPLMRRRPFRAPHHSI
                     SEAGLIGGGTIPRPGEVSLAHNGVLFLDEAGEFGRATLDGLRQPLEDGHVTVTRASGS
                     LRFPARFMLVAAMNPCPCGYYGDRTKDCVCSAAQVRRYRGRLSGPLLDRLDLQIEVPA
                     VPIRALGDDVATSDSSAVIRSRVMAARARQAERYRGDGIYTNAQLKPRHLKQYCALDM
                     QSRELLEQAMTRLGFSARAHGRILRVARTIADLAESDSIGPAHLAEAIQYRSFDRRVE
                     L"
     gene            43028..43600
                     /locus_tag="PK224_11385"
     CDS             43028..43600
                     /locus_tag="PK224_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16383.1"
                     /translation="MTALSLALLFPAIALSQPWNDKPGSTMQPVAQPSRTSSVYIGSV
                     MALLATFEDAGILPPEGTPQANGIIKAVIQFQSAFLKSHHPAIRQFFTDAHRVKLGPR
                     AEEVETSFRRSGWSADTFDAVMAVGQTDNAWNTEGLSEGFREFNIGKPDFDILARLYE
                     QSRAAFSTQGKTFQEVYAQRRREMPGAKSE"
     gene            43673..43909
                     /locus_tag="PK224_11390"
     CDS             43673..43909
                     /locus_tag="PK224_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRB16384.1"
                     /translation="MATKAYILIKVKAGKGKSVLSALKSIAGVEQIHACFGQPDIFVF
                     INVADERALSDVVITRIHAIEGVEETDTHIVAET"
     gene            complement(43910..44899)
                     /locus_tag="PK224_11395"
     CDS             complement(43910..44899)
                     /locus_tag="PK224_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB16385.1"
                     /translation="MSHEPGLPLGIGLIGVGHHGSRYAKHLLQDLPEALLAAVSRRRT
                     AGGNGLASAPAVPCYPDYHELIADPRVAAVVVVTPPALNREICLAVVQAQKALLVEKP
                     LAISATDARAIAQAAQRSGQITMTAHTLRFDAAVGVLRERQAQIGALQYLSLASRMEP
                     HGMSEDGRGFGGRGCLLETGIHLLDLARVLTGDEILTVSCEMDVVPPDGAERRIVGRL
                     TTSGGLVCLVDVSRVSSGRVGRVELIGSEGQLSADWCGQRVAQVSARHGAGNWPSIAE
                     PTVLGTLRAFVKSVRNGLPPPVTIEDGKRAVEIAEACYASARQGGRVVPVEYL"
     gene            45122..45844
                     /locus_tag="PK224_11400"
     CDS             45122..45844
                     /locus_tag="PK224_11400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRB16386.1"
                     /translation="MAIPTATDAMVDPYIRTRVSKDVQVECIQFWPQDKTAYPGIVLL
                     HDWWGMNSQITDLGARLACEGYGVIIPKLYGRLGGMVTANAEVAEALMAKCNEKLLLQ
                     DINTCCEYLNTTEHTKRNIHGVVGFGMGASLAIRFACQRKRLRAAVAYYGKVTAPDQL
                     TNMMSPLLYHQAELDTWATQQDVDHLRAAASQGKRIEIKTYPGASHAFCDETRPAGYQ
                     PEAATQAWDTTVAFLKTSFQGT"
     gene            45934..46908
                     /locus_tag="PK224_11405"
     CDS             45934..46908
                     /locus_tag="PK224_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16387.1"
                     /translation="MPNHSVRTLLIACCVTALLTAAVVAGADEPSPQAKPTLANRLPA
                     FHDLVRVDAAPLAALPIQGDSLSLFKTVVGPQLGLNDVAMTLGAKGLTPAMSQELLIP
                     DLTKSANELVRGLAAWQLTQAAKDSSTSAGTEQRESLQRQIESQMTWLTEGTTLETPL
                     RQLLALLKEFPTQEAAAPLQGTAAQIEAWAIDTVHREWFRLYNWKDQVRQQRGLTRLC
                     GTWQWSIHNHQNHREEKTAVIFAPPGIASSTSPAEIIVLGDSVYLRWETRAGVQEDSL
                     LFSGEGQRLEGTFVNTAGGWGSITGKRTATCVTDGGGKPAPSPRRQHH"
     gene            complement(46902..47297)
                     /locus_tag="PK224_11410"
     CDS             complement(46902..47297)
                     /locus_tag="PK224_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRB16388.1"
                     /translation="MAVSARSFRFVFLGGLFAATSVAAGAFGAHALKSILDPSMLAVY
                     ETAARYQMYHALGLFVVAWLVRETEDSLAVKAGWLFCIGILLFSGSLYIVALAGIKWM
                     GAMTPFGGVSFISGWACVAWTAWRAERVQ"
     gene            47409..>47756
                     /locus_tag="PK224_11415"
     CDS             47409..>47756
                     /locus_tag="PK224_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16389.1"
                     /translation="MANPDAAQYCECGYDFTPHFQGFPIKPASDITVRKRLFPDEMGR
                     GSFLLGILLIWLALSVGELYRIRIELPRRPKMELGLSSLMVGMTDLAAALVVGAIGWA
                     CVWIVLAAQSKRDG"
BASE COUNT        10044 a        14564 c        13147 g        10001 t
ORIGIN      
        1 cttgatgaat cgcccgttca cggccagatg aatctggaac actcgacgtc ccagcggttc
       61 gtctgatcga ccggcgctga cttgcagatc gaccgtcccg gagggtacga cgatcggctg
      121 ctgaggatcg ccgatcgcca cctggcagtc gacggcgcgt ccggccagtt cccgtttgac
      181 gaaatcgtgg atcaccccgc ggatgtgttc gggatagatg agccgtttcc cacgggcctg
      241 cggaagaccc tgcgcctgat gcgcaacagc cccctgcacc tggaacagcg gccggaccgg
      301 agcccgctca cctgccatgg ccgggaggac ggcgctcgtg accaggctga tggtcgcaac
      361 agacaggagg ctgagtcgat tcacaggtgt cacccttggt tgaaggtgcg gcgccgatta
      421 ccggcgcagg ttgttggcaa tctgcatcat ttcatcggat gcctgaatgg tcttggagtt
      481 aatttcgtaa ctccgttggg cgatgatcat gttgaccatc tcctccgcca gattgacgtt
      541 tgaactttcc aggaatccct gctgcaaggt gccgaagccg gtcgagaatc cgccggtacc
      601 ctgttgcgcc ggtccggagg caaagctgtc caggaacagg ttgccgccca tcgccaccag
      661 gcccgaaggg ttgtcgaagc ggaccagctg aatctgcccc acctgcgacg cttgtgtgac
      721 gccgggaagc agaacggaga cggtgccgtc ctggccgaca tcgattttca gcgcgcccga
      781 cggaatcgta atcaccgggt tgagctggtc gccgtcgccg gtcacgaggt taccgacgtt
      841 atcgcgcttg aaggatccgt tgcgggtgta catgatggtc ccgtcgggcc gggtcacttg
      901 gaaaaatccg gcgccgtcga tagccaggtc gagatcgttc ccggtctgcc gcatgttccc
      961 ttgcacccat tccttggcca ccgtaatggg acgcacaccg cccccgacct gcacgccgac
     1021 aggaaagacg cccacgttcg acgcgttcgt gcccggtaag cgttgaatct gatacagcag
     1081 atcgccgaat tccgcacggc tgcgtttgaa cgcgttcgtg ttcacgttcg ccagattgtt
     1141 ggcgatggta tccacgttca gttgctgggc cgtcatgccc gtcgcggccg tccacattgc
     1201 gcgaatcata cgctcctcct attgcacccg gcccagatcc tgaatggccg tttcggtcat
     1261 gcgatccatc gtttggatca atttctgcgc cgactcgtaa ctgcgcatgc cttgcatcat
     1321 cttcaccatt tcactcaacg aattcacgtt ggactcttcg atgtgtcccg actgcacctg
     1381 cggccttgtc gacaccgtcg gtttccctcc gacaaacagt ccttcggcaa acttttgcgg
     1441 catcgcgttc tccgggaact cgaccacctt gatgctgccg atcggagtgc cgtccacctg
     1501 gatcgcccct tccgcattga tctgaatgtt gccgggcggc accttgattt cccctttggt
     1561 gcccatgaca gggtgcccga gattggtgac caggcgtcgt tggctgtcga gagaaaagat
     1621 gccgttgcgc gtatagcgaa ccccgtccgg ggtattgacc tcaaaaaacc cgtcgttctg
     1681 tatggcgaca tccagtggat taccggtaat gcggatccgc cccggctcaa aggccgttcg
     1741 gaccccatgc acttcggcaa agacccgttc gctgggaccg gccggtctgg tcgaaatggc
     1801 ggaggagagg cctcctgaca tcactcccat ccccgtcgaa ctggcccggg cgaaaaggcc
     1861 gcggaatccc tgctggtcct gcttgaatcc ggccgtgttc acgttcgcca gattattggc
     1921 gaagaccgtg agttgttgtt cctgggctac ggcgcctgag agtatggggt agatggctcg
     1981 attcatagtt tgtactgtcc agatgaacct gttgtgctac agggggagtt agcaatggac
     2041 atgccatcgc aatacgtcag acggcctggc acagccgaag cgatcgccgg acgaaaatcc
     2101 ttgtgattgt cgagagatgg gaaccggaag gtatttgagg ggccggaggg aaattcttcc
     2161 gccgtcaacg gcgcctgtca aacggcgtgg caaacgggaa gtctttgccg gaagcaggga
     2221 aagtcctgcc tacatggaat ggggcgcaac gacaattctg gagatcacgc agcgatcgtg
     2281 gaggttgcat caacggaggc ggataccatc gcaccgggga gtccatagag agcgtaccct
     2341 tcggcacgag ccgttgcgac ggcccgggtg gccacatcga ggagcgcgtc aatgtccata
     2401 gtgtcctggc tggagaggct gattcctatg cggctggtga ggaacaattc gtgcccgtcg
     2461 agggtgaccg gcagcgatat cgtgagttgc atcttcctgg cgatggccag cacctcctcc
     2521 ggacctgtga cctgatccag caggacgaca aaggtgcgtt cggcaattcg cgctaccgtg
     2581 tcggtggtgc gcagggcgct cttcacacgc gctgcgtgaa cgcgatacat gaggttgagg
     2641 ttgatctgcg cctgttcgtc ggttttcggc gtgaagtgat ccagttcgat catcaggacc
     2701 gccacgggcc gcccatactt gtgagcgtgc actaaggctt gattgaggag agatgtaaag
     2761 aggcgatagg tcggcaatcc ggtgaccgag tcgtaggtgt tcatgccgga cccgttcgat
     2821 tcttccgaca gtgaatgcat cctggaagcc gattgtgggc cccgcggagt ccagagtgtc
     2881 gcgccaaaga ccatcagcgt gaggagaatg ccggcggaga tcatgacggg ataggccgtc
     2941 aacgccgaca gagtcaggag gcccacggag gcagtccaag acaatccgcc ggccatgaac
     3001 aggatacagg ccactacgcc gagtcgagcg ctctgaagcg attcggtacc cgcttcgtcg
     3061 tcgggccacc catgccggtt cgttgaatcc gacccgttgc ggtcgctcga ggtgttgagt
     3121 tcaggacgtg acgaatgcat accctcccca atggagcatc gcagccaacg ggatgttgag
     3181 ctaggaaggt atcggagaag tggaggcgaa actaaaggca gtgtagcccg cgcgacgatg
     3241 catgggcgcc gccgacacga acgaacagca acgactgcta caccgtccgg aggggcacat
     3301 tacacatcga ccggttcgag ccgcgctttc aagtggagga tggctttcga atgaatctgg
     3361 catactcgcg attcggtcac cttgagcgcc tgcccgatct ccttcatcgt gagttcctcg
     3421 aaatagtaga gcgacaatac caaccgttct ttctccggca gatcctggat tgcagcttcg
     3481 agcacgtggc gtgcccgttc gctcactagg accgagagcg gatccggctg attggagtcg
     3541 gtcaacacac tgatgatctt atgcccgtcc gcatcctgca cgccgagatc gtccaggctg
     3601 atcaccacgg cgccacgtga gcgagtcagg aattcatcca gttcctccgt ggacatcttc
     3661 agctctttgc cgacttcctg gtctgtcggc gggcgtccga gtctgtgcag cagctcggaa
     3721 taggtctttt gaaggagcgt aatccgctcg tggaccgagc gcgggatcca atccatcgac
     3781 cggatttcat cgagcatggc gccacggatg cgaaactctg cgtaggtctt gaatttggct
     3841 tcgcgggtcg gatcgtactt atccatggca tccatgagtc ccatgattcc gacggacatc
     3901 aaatcttccg cgtccatata ggccggcaag cgaaacgcca gtcgatgggc catggccttc
     3961 acgacgtggg tgaattcctg aatcacccgt tcacgatccg cttccgcaat cacccgacgc
     4021 ggggcgtttc tgaccgcaga catttttcga gcgtccatca ttcgctacag tcctttcagt
     4081 cagtgcgtcc gcaaaagttg ttgccagagg aactgcactg tgcctttggg aagcgccggt
     4141 ggggttaact gagccaccgc ctcagtcagt tctcgaaacg cacggctgga cggcgcgtgc
     4201 ggaaagagat cgatgacggc ccgctgctgg gtcacggcca tcggcacata gtcgtccaag
     4261 ggaatcgcgc ccaggtaatc cagcgaaata tgcaggaatc gactgacggc caactccagt
     4321 ttccggaaga tccgcgcggc gtcccgcggc gacttcacca tgttgacgag cacatggaag
     4381 cggcgttccc ggtattgccg caacaggact ttcatcaacg cataggcgtc cgtcatggag
     4441 gtcggctcgg gagacaccac gacgataatg gattgggccg ccgccgcaaa gtaggtcacg
     4501 gtcgaagaaa tgcccgcccc ggtatcgatc aacaacacat ccatgttcga tgccaagcgc
     4561 tcaagttcat cttgaaggat gagctgttgc gcttccgtca ggatcgtcaa ctgagaaatg
     4621 ccggtactgg ccgggaggac ggtaatgcca tgcgggccgg tcagcgcgat atcttccagc
     4681 cggcaggtct tcgcgagcac gtgctccaac gtatatttcg gtgttagacc gagcagaata
     4741 tcgacgtttc ccaacccgag atccgcatcc agcacgagca cacgctttcc ggtttgggcc
     4801 agggcgatgg cggtattcgc caccacgttg gtcttcccca ccccgccttt tccgctggtg
     4861 acggtaatca cttgcgtgcg ggggggaccg acgttgtcat caagcgctct catctgcgga
     4921 tccaaaattc ctggttgcat cgccatcgtc actcccctct tgttcgtcag tcgcctatcg
     4981 cgcgtaggtc ttgataccag gcacaactgc tgatgtcgtc tgccgatccc atgactgagt
     5041 ggtcccgccc gccggcgtcc gtacctgccc ccccagcagc aggtcgccca ggcgatcgac
     5101 ttgcgcctga accaagtcat cgggcacccg ttgaccggtt ccccaatagg acagcgggat
     5161 gccggtgcga tgagtggctt cgaagatgcc gccatagccc gccgtctcgt cgagtttggt
     5221 aaacaacaac cggaggcccg gcacgtctac gcattgactg gtgctgacca cgagatccgg
     5281 cactcgggtg cctgccgcga ggacgagatg gacctccatg ggataggccc cgttcagcaa
     5341 ccctctccaa cgttgcgtcg tgatgggttc atagggattg aaccctgtgg tgtcgatcaa
     5401 aatcagttcc gcttcacggg cctgcgcaat cgcggcgcgg gcctcctcac aggatgctgc
     5461 caccgagagc tcgataccca ggacctcggc atacaagcga aggtgttcca ccgccgcagg
     5521 ccgatacgta tccatggtaa tcaaggccac actccgctgc tcgacaatgc catagtgcgt
     5581 cgccagtttg gcgatggtcg aggtcttgcc gactccgctg gggccgacga acagcgcaat
     5641 cttttgttcc cctgccgcgc tcagcaacgg gccggccgtg ctgacccgat ccaacaacac
     5701 tgtccggagc aactgtagtg aggagttccg ctgagcggac ccctgctcca ccaacgtttc
     5761 acgcagttcc cgcagacagg cttccgccgt ggcatgctcg acgccctgcg ccaagaggtc
     5821 ttcatagacg cgctggaagg actggaacgt tgcgccctgg ttctccccat tgtggcgtgt
     5881 ggacgccggt gtcgcgcgag tctctcgcga aggaccggga acctgacgca ccgcaggtcg
     5941 cgactgctcc ttccccgttt ccatcgctcc ctgcagggtt ttttgaaaac gcgattcctc
     6001 cgccggcacc atcggcgcag gagtcacggc cgcacgtgtc tgctcatctt caccgagcag
     6061 cggcgcggcc tcgggaacgg cggcgtcatc ttcaatggcc gccatgacct ccaataccga
     6121 cccgcccaac aacccgaatc cgttatccca ggaacgcacg cgtttcgtgg acagaatcac
     6181 cgcatccggc cctaacgtgt ccttaatgga gcgaatggcc tcatgcatcg acttgacgtg
     6241 gaatgtccgt accttcatgc caagctcctg gtttcaccga cctcgaggtc gagccgtacg
     6301 gtttccactg cctgcaaacg tgtcacgctt tccacctcgt tcaatcctaa aatcggaacc
     6361 gagtgcaaaa ttctgtccgt cagtttccgc agatgtcgtc gcaacgaagg cgagcacaaa
     6421 atgaccggtt gatgcccgcg accgaccatg cgttccgcgg cagccttcaa tgcagtgagc
     6481 agtttctgtg ccaccagcgg atcgggcacc cactgggttc cctgtccggc tgcattcgct
     6541 tgatcggcga gggtacgatc caatcgcgga tccagtccga tgatcggcaa cgagccatct
     6601 ggcgcgagat attgcttcgt gatggttcgt cccagcgctt gcctggcgac ttccgtcaaa
     6661 atatcggcgt cttttgtggt cgccgcatgg tcggcaatag tttcaagaat cgtccgcaag
     6721 tcacgaattg ggacaccctc tcgcaggaga tgcgacaaaa ctctgacgag cgtgcccaac
     6781 gggatgagat ttggaatcac ttcctcaacc agcttggggt gatttttgcc cagttgatcg
     6841 agcaagccct gcacttcttg ccgccccagc agttcatgcg catgccgctt gatcaactca
     6901 gacaagtgcg tcgcgatggc cgatcccgga tccacgacgg tataccccgc catttgcgcc
     6961 tgttcacgct gctgctccgg tacccacatg gccggcagac cgaacgccgg ttctttggtc
     7021 ggcagcccat ggatagctcc tcgctgagcc gtaccaggat cgatcgccag cacatgcgcc
     7081 ggcatcacct ccgacttggc gagttccacg cctttgagca gcgccgcata ttcgttgggc
     7141 cgaagctgca agttgtcgcg aatatggatc ggcggcagga caaagcccat ttgttcggcg
     7201 aactgtcgcc ggagcccctt gatccggtcc agcagggcgc caccctgccc tccgtccacc
     7261 aggctgatga gaccgtatcc gacctgtact tccatgagat ccagggggac cacctgggtg
     7321 ccggcctcct cggccttgac ctgaggcgag gccggtgtcg gaacggcttc cgcggttttc
     7381 tgctgttcgt tgatctgata cgccatccag gcggtcaggc cacccaacgc gaggaatgcg
     7441 agatggggaa ggcccgggac caggcccagc gtgatcagaa tccctgaagc cgtgccgatc
     7501 gccttcgggg agatgaggac ctggcggctg atctcaaatc ctaagttgac ctcggaggcg
     7561 gcccgagtga tgacgatacc ggccgcggtt gaaacaatga gcgccggcac ttgcgccacg
     7621 aggccttcac ccaccgtcaa caacgtatag gtctgcgccg ctgccgccag cgtcatgccc
     7681 tgttgcaaca caccgatcgt caacccgcca agaatgttga cgagcgtgat gatgacggcc
     7741 gccacggcgt cgccgcgcac aaatttgctc gaaccgtcca tggcgccgta gaaatccgct
     7801 tcctgggcga tctccttccg ccgttggcgg gcctctttgt cattgatcag gccggcgttc
     7861 agatcggcgt cgatgctcat ctgtttgccg ggcatcgcgt ccaaggtgaa tcgggctgcc
     7921 acttcggcga cgcgcccggc gcccttcgtg atgaccacga agttgatgat gacgagaatg
     7981 gtgaacacca cgagcccgac ggtatagttg ccgccgacca cgaacgtgcc gaaggcccgg
     8041 atgacctccc ctgcagcggc ggcgccttca ttgccgtgca ggagaatcaa gcgcgtggag
     8101 gcgatattga gggcgagacg caggagtgtc accatcaaca gaatcgaggg gaacaccgag
     8161 aattccaacg gacggcgtac ctgcatgccg accagcagaa tgatgatgga cagcgtgatg
     8221 ttgaaggcca ggagcagatc cagaaaaaac cggggaagag gaatcaacat gaccatcaaa
     8281 atgcccacaa cccccacgga catcatgatg tcggggtgtt tgaccaagga ggtcgtggaa
     8341 accgatgtgt tctcgttcgc catctagact ccaatccaat cggatctacg ccgacggcag
     8401 tttgccgcgc acgcgataca caaacgccag gatttcggcg actgcacgat agagatcctc
     8461 gggcacttcc cggcccacct cgaccagttt gaagagggtt cgtgcgacca gcttgttttc
     8521 gacgatcatg actccgtgtt gccgaccgat ttcccggatc ttttccgcga caaagccggc
     8581 gccttttgcg acgacgatcg gcgctcccat cgccttggag tcatatttca gggccaccgc
     8641 caggtgtgtc gggttggtga taatcacgtc ggccttgggt acctctgcca tcatgcgttt
     8701 ccgggacatc tctctctgag tgctgcggat cttggccttc agccctgggt ctccttctgc
     8761 cgcccggctt tcctctttga tttcgtcgcg tgacatgcgg aggtcacgct cccattcaaa
     8821 ccgttggaag ccatagtcga ggccgccgat caccagggcc gcaccgccca tcatgagcgc
     8881 agctttgaat gtcgcccacc ctaccgtcgg caagagcgtc tccatgccga attgtgtcag
     8941 aggagagaac aacagcatat cctgcttgat cgtgagatag ccgactcctc cgatggcgaa
     9001 gatcttgagc cacgatttca ccagttcgct gaaggaccgg acggagaaga gccgtgagaa
     9061 accgcccatc gggctaatgc gggaaaactc caactgcagt ccgtctcgct tccagaggaa
     9121 accggtttgc atgaggttgg cacccacgcc gaccaccgcg atcccggcaa tgaccgggcc
     9181 gagcatgata aacacatcga tcccgatctg ccttaagatc aagtgcagat ggtcgagact
     9241 cagtgtccgc tgtgtggact cctccatgga tttgctcagc cacaactgca gcgactctcg
     9301 aagcttgttc aggatgcccg gcgtcatcca atagagggcg cccaagctcc ccagcaatcc
     9361 tactgccatg gcggcatccc ggctgagagc gacctggcct ttcgccctcg catccgcttt
     9421 gcgtttcggg gttgcccgtt ctgtccgatt ctgtccgctg tcagccatga ccgagtgcct
     9481 tcaggagtcc gagaatggtt tccaccaggg tttcgaattc tttttcaaac aacaacaagg
     9541 tgaagggcat cgccaggctc atcgcgagga agcccgcccc gatggtgatc ggaaaactcg
     9601 tgacgaacac gttcatctga ggtaccgccc gaccaaggat tgccaggacg gtattgacga
     9661 tcaggagagt cgcgaagacg ggagccgcga gcttgagggc gacccccagc atgttctgcg
     9721 ccaggtgaat gatatcctga gcgatgccgt cggataacga ggcgccgaac ggcggcacgt
     9781 attcgtagct catgccgatc gcatgcacca ccagcagatg cgcgttgaga gagaggaata
     9841 ccaaggagcc gagcgtgagt tgaaattgtg caatgatcgg cgtgttctgg tggctcatgg
     9901 gatcgaacat ttgaatggcg ctgaaaccca tctgcgtgcc gatcatgtca ccggcgacct
     9961 ggaagcccga gaacagcagt cgcaccgcca aaccaatcgt cagtccgatc agaaactcgc
    10021 tgccgatccc agcgatgacc aaggccggat ccgtcggcag gctcggcagg tgaatgattg
    10081 gcgccaagac caggcccagc gtggcgacga gtcctgtttt gaccggcatt ggaatcgtgc
    10141 gcgtattgag gataggaaag acgctgacaa tgccggcaat ccgcaccagc aggatggaaa
    10201 aggcctggaa ctgaggcagt gcgagatgca gggtttgcgc agcgctcatc gtggtaccgg
    10261 gcctatcagt gaatcaggga cgggatactc gtgatgatat gggtcataaa tgcaatgagg
    10321 acacccatca tccacggcag aaacaagagt gtggcggcga ataccgccag caccttgggc
    10381 acaaaggtca acgtcgcttc gttgatctga gtcatcgcct gaaaggtgct gaccagcaac
    10441 cctacgatca ggctcaaacc caagatcggc gccgacacca gtatggtggt ttcaatcgct
    10501 tggcgaccga tttctgtgac tgtttcaatc gtcaagtgtc gcgctcctac tcaaagctcc
    10561 tgaccatcga tccgacgacc agataccagc cgtcagccag cacgaacaaa atcaatttga
    10621 acggcaagga gatcatgacg ggcggcaaca acatcatgcc catagacatc aggacgctgg
    10681 cgacgaccat gtccacgatg aggaacggaa tgtagaggag aaatccgatt tgaaacgcaa
    10741 tccgcagctc actcaggacg aaggcaggaa tgatcacgtg gatcggcacc tgttctactt
    10801 gcgtcgcttt gggcgcatgc gacagctcca tgaacagttc gagatccttg tcgcgcattt
    10861 gtttcaacat gaacgcccgg atcggctgaa tacctcgatt ccaggcatct tcgtacccga
    10921 tttgctcggc gagcagcggt tgcagggcat ccttgtagac ggcctgtccg acgggagcca
    10981 tcacgaacat ggtcagaaac aacgcgaggc tgatcagaac ttgattgggc ggtacctgct
    11041 gcgtccccat ggcctggcga agaaaggaca gcacgatgac catacgcgta aacgacgtca
    11101 ccatgatgaa caaggcgggc gctagggaga ggacggtgaa cagggccaga atctgcagga
    11161 ccacggcagt ctgcttgccg ccgccctggc cgagatcgat cgtcagggaa ggcccttcgg
    11221 cgtatgcgaa ggagacgctg aacaggcagg tgagcaggat cagactgatg aggagaccgg
    11281 cccaccatcc ggaacgttgc gcggccgacg cctgtctcgg cgggatcagg agactagctg
    11341 tgtgcacgtg ctgtctcctc aaggttgccg gacccgggga ttccggtggg tgctccggag
    11401 tgcagcgcgg acgcctcggc aatgaggcgt ttcgccactt caggatcggc gatctttccg
    11461 agcggcacca aatcggtggc ggtagtgccc aggatcaaca cttctcctgc caccgccaca
    11521 accatcacgt gctttctgga tccgagcgga ccacttccca aaatcttgac caacggtgcg
    11581 ccattcacag ggagaaaccg acgcccgatg gcggatcgcg ccacggccag cagacccagg
    11641 atcaatgcca ggacgacccc gagcgccgac accatgcgaa ccacgctttc ccaaatctcc
    11701 atggggatcg ttcattcctc tcgccgccgg agcggcggca ttgccgcatg ttgcgccgac
    11761 tacccgagtt gctgaatgcg ctgattcgga ctcacgacgt ccgtgaggcg aatcccgaac
    11821 ttttcgttga ccacgacgac ttcgccgcgc gcgaccaatt tgttgttgac caacacttcc
    11881 atcggttcgc cggccagctt gttgagttcg atgaccgacc cctggcccaa ctgcagcaac
    11941 tcccggatca gcattcgtgt cgagccgatc tgcacggtca cttgcagcgg aatatcgagc
    12001 acaaactcga tgttcttgct ggcgggcacg gcgttctgat tatcaacagg gggaaacgag
    12061 gtcggttgca cgttgatgtc cccggcgccg gcattggaag cgtccgacgg tgcagttgtg
    12121 tctccgttgc ccatgcgcaa atctccttta gttccgggaa gctctacccc aatatcttgg
    12181 tcacgcggca cgcatgattg cccttaaaga tcccgggctg cccttgaaat ttcggatcgc
    12241 cctcgaccag gcacagcatc ggatcgccgg gatgttggtc gagcatcaac acatcgccgg
    12301 ggttgaaatt gagcaagtcc tgcacattga tacgcgcggt tcccaactgc acggtcatag
    12361 ccacgtcgca ttcctgtaag gaatccttga aacgactgcc ccaactgctg tcctgctcga
    12421 cattgtcggc aaacaaaccg gaatacagtt tttctttgat cggctccaac atggagtagg
    12481 gatatgcgat aaacatttcg cgggagatgt cgcccaggtg cacctgcaac gtcaccacca
    12541 cgacgatttc agacgtcgtc acgaccatgg cgaactgcgg gttgctctcc gatcgtaagt
    12601 acccgatctt caccggcatc acggcctgcc aggctttctc cagatcggcc agcgcctgct
    12661 gaaccagctt cttgatcaga cgaagctgaa tcgtagtgaa ctcccggcct tcgggtttca
    12721 catgcgtttg cacttttccg ccgaagaaga agtccacaag caggtacacc agcatggcgt
    12781 ccataacgta gagtccgttg ccgcgcaacg gctccatggc gaacagattg aaggaggagg
    12841 gaactgggat tttgtggatg aagtctccga acttcatgat ttgagcgccg aggacgctga
    12901 attcgatctt ctcgcgaagc acggcgctcc aggagatgga ttgctgacgc gtgaagcgat
    12961 cgttgatcat ctccatcgtg ggcatacgcc cgcgaatgat gcgttcctga ctggtgaggc
    13021 tgtacgcttt tatcccgccc ggatctacct cctccttcga agccgtctcg acttcgccgg
    13081 acatgacgcc acccatcaag gcatcgattt cttcttgaga gagtatcttg tccatcacgt
    13141 cctcaagaga gtcgttgcgt tactgaatga caaagtccgt gaagtacgcc atcttgaccg
    13201 atttcttggg aaggaggccg ttgatgcgct gcgtcacgtc ttcacgcagc ttgtatttac
    13261 cctgcggaga acgcgcggtc acagaatcaa gactgctcaa caaaaccagg attgcatctc
    13321 gaatctgcgg cgtgcggacc ttgacatctt cgacggcgcc gaggttttcc atttcgagtt
    13381 ttatcgtcac tttgaggtac cgcacttccg gcgaatcagc tagattgacg atgaagggat
    13441 ctaggtcggc gatcgacccc gtctcgcctc ccgcagcatg cccgccttct ttcgccgcag
    13501 cttctccgtg agccgccgat gtttctgccg caccttcagc agccggtttc tcggacgatc
    13561 cggacatcat tttgaacatc ataaatgctc cgccgagcgc gacgatcaac gtccctccca
    13621 ggaggataat aaccattttc atgggcatcc ccgcgggcgc ggcggacgcg acaggcgcct
    13681 tctcttcagc tgcttctgac atacggacct cctgcggaca gtgtttgccg gtgccggttg
    13741 attgtgcaat gcatatgcca cgcaacgacc ggcaacagtc ttaggggaat ttcactctgt
    13801 acaccggcac cttcgataac cccctgattt cagggcacga cgtcttccgg acgcgcgtca
    13861 tcgtcgaccg ttcgtgagac cctgtcagcg gagtgacgcg ggcttcacgg gagccgacag
    13921 aatgttgacg aaggtgggcc aacgtgaggg gctgtatgct ggagaggcgc taggtagaaa
    13981 gtgccgactt gggccctgac tcgccctctt cagacgcatc agggccctcc tcgcttaccg
    14041 tttgagattc acgagttcct gcaacatttc gtcgctggtc gtaatcgcgc gtgagttggc
    14101 ctgaaaagct cgctgggtaa tgatcatgtc gacgaactct ttgccgaggt cgacattcga
    14161 ctgctcgatg gttccggaca agattctccc caaaccattc gtcgtcggcg caccggataa
    14221 cggcgcgcca gagtcaatgg tttcaaggaa atgattgtca ccgttgctga ccaaaccatc
    14281 tggattcaag aaacggttca gaaccacttg cgccagaggt cttgtctgcc cattggtaaa
    14341 ctgagccacc acgtgtccct gcttgtcgac gctgatgcga tcaagtgttc ctgaggtaaa
    14401 tccatcctgc gtttgactga tgataccgga cgcggacccg aattgagtca ttgcggcaag
    14461 actcaccgtg acagcttgag gtgtcgtggc gccgttggtc aaagcagcgg tcacggtctg
    14521 tgttcccccc gccgtcagcg ctccactggc attaaacgtc atgttggttt gcgcggcagc
    14581 cgcaccccca tcgatttggg agtacactcc ccaggtattt gccgccgtct tccggaaata
    14641 caattgcatt tgatgggagt tgccgaggga atcatagaac gtcatcccgg tcgagaaatt
    14701 gtaggaggtc gtatcggtca cgctgaaggc tgtcgtgggc gccgtggcat tggcattcag
    14761 gttgccgaga atcgccgcag tggtggttgt ctgtggcgcg accgcactgg acgacagcgt
    14821 gatgctgccg atcgtgctcg taatgttgcc gttcgtatcg gcctggtagc cctgcaacag
    14881 ggctccgctg ctatcgacga cctgcccaag attgtcgacc ttgaactgac cggcgcggga
    14941 gtagaagtta gcgccagcac tgtctcgcaa gagatagaat ccgttcccat cgatcgccag
    15001 atcgaatgtg ttcccggtgg tggttattga tccctgtgta aagttggtct gaacatcatt
    15061 catatacact ccgaggccga cttgatcgcc accgcttccc ccggccaggc tggaggaaat
    15121 caagtccgaa aaggtcgccc ggctggactt aaatcccgca gtccccacgt tggcgatatt
    15181 gttcccgatc actcccatgg cgttgccgta ggagctcaag ccggtcactg ccgtgaacat
    15241 cgatgttaaa atgcccattg tctcatcctc cttgttacca ctacctgaaa cgttctctcc
    15301 aagcgttcgt ccgcaccgca gggcgatggc cgttatgcga cggccgtcgt gccggtggtc
    15361 gcggttttgt tctgcgcgtt ttcctgaagc gtcacactct tctccatcaa ggacaccagc
    15421 ttggacattt gttccagctg cgagaactgt gcggtttgcg cgacgaagtc ctcattttcc
    15481 atcggcttca gcggatcttg atgctgaagc tgggtgacga gcagcttcag gaaatcgtct
    15541 tttcccagcg gcttcggacc atcggtggtc ggcgtggatg agtctggggg tgtcgcctgt
    15601 gtaatcacct tgctgatatc catgggacct ccaatcatgc aaatatactg acgcgttcat
    15661 tcgtgcagtc gccggtctct cccctcgcac tgcgctcttc ttctcccgtc gtggaccgct
    15721 ggccggacga ggtcgtcggg cgatcctgat gctgctgttg aaacatccag gcattgtcgt
    15781 tccgaccctg ttgttgatcg acggtgaccc gcaacatgcc catttcgagg cctgtcgtgc
    15841 ggagcgatga ttccagtgac tgcccctgtt gcaggaggcc ttgtcccagt tctccatgtt
    15901 ccgtccggat gtgcgcatgg acggtttgat cgctcatcat gacacggacc cgcaagggcc
    15961 ccatgtccag gggatccaag tccagcatga cgctttgtga gacgggcgag gctcctcgaa
    16021 gctcgctcat gcgttcggct tctgctgctc gcaggactgc agcgggctgg ccggcctcgt
    16081 tgcggccgac ggcgctttcg gagagaggcg gggccggttg attcagtcca ctcatccggt
    16141 ccaggaacaa cggacgatcg atcggcccgt tgctcatggc ttgattcggc atcgagcgat
    16201 cggcatcggc agaagagtcg gaaaacggct ctgactcatt ctcgagaaat ttcccggcgt
    16261 tcgcacttcc ctgttcggtg ctcaccggac tgtcctgtgc gagatcttca agaaatgtcg
    16321 gccggtcaac ccgatcgccg tcagcctgtt tcatgcctcc tgcctctgcc gtgcgtgtcg
    16381 tgacagccgc agcgacagtc gtcactttct ccggatcgat cgtgctcgcg gtgacggccg
    16441 cgtccgttcg atccgcgctc acttctctcg cctcaccatg actgtcggga tgagggaacg
    16501 ccgacatatc aatcattccc tcgcctggct gttctttttc agcggattct tttatgtctg
    16561 acttctgcgc ctgggccgtt gcagcctggc cgacgagtgc ctcttcagat gtctgtaccg
    16621 agggatccag cgccgcgagc tgagcggaag gcgtctcgtg agggcctgtg gcggctgtag
    16681 gcgccacttt tccggcgcca tccgcagagg ggtccaattc cgaatccgtt tcaccactga
    16741 tctgtacttc cgtctgtgcg accgcaggcg tgggaagaag caacgcggcc acaaggatct
    16801 cctgccgttg cgtgctctga tcctcagcct gttcagtatt gtcttcagca ttggggctct
    16861 cgacttccgt gtcggacaca cagtcagcct tcacacaagg ctcttctttt aggctggcag
    16921 ctcgttcctg ccgtgcgaac tccctagagc gagcagctcc ttgcttgtct gattccacct
    16981 tggaatgatg cgacgaacga gtcggtgaag ccggtttcga tgcggctcga gctgaggccg
    17041 gttccggtgt catcgccccc cggtgggctt ctgctcgctc aagcaacgaa tcgaatgtgc
    17101 gagatggcgc cgaaccgtga gccgtcgcct ggcgttctcg cgcgactggg gcgccatgag
    17161 tgctgtgact tggaattccg ttcaagaagg gctgcatgtc agtggccata ggtgttcctg
    17221 tccgtttcga accccgtacg ttgtcatgga acagcattac aaaggccgtg ccatccacgt
    17281 cgaacgagaa aagcgcgcat cacacgggat ttttgcaagg aggactaaac gcggagactt
    17341 gaagagggaa gtttctgcag gaggtgacgg caaaacctgc cgaatcaccg ggacacgaag
    17401 ggtctgttta cggggccgtc agcaattgtt cggtcaggcg ggcggcgcgt tccggtttga
    17461 cttcagcgag gatcgcgccg gcagatttgc ccttaatcag gcgtaacact tcgagcgcct
    17521 tcctatccgg catacgctcc agacgggcgg cggcttcctc agccggcatc gcttcgtaga
    17581 ttttcgcaag ctgggtctga ttgacgttgg ccgcccctcc cggtttggcc gtcggcttgg
    17641 gcttctccgc ttcactcgtc gccttgttct gggctgcctg acgacgcttt ttttcagcct
    17701 caactgcctg ttcatgccgc gtcacaatct gttcgagttc cgctttgagt tccaggagat
    17761 gcatctccga tccgcgtaac gccgcctcac gcttatccag ggcccgtttg cgctgctcca
    17821 gcatctgaaa aatttcacgc ggcgtgctga tggcaggacc ttggacctct tttttgatcg
    17881 cgtcggcttt tggttcttcc ttcgctgctg ccggagccgg cgccggagca ccttcatccg
    17941 cgacgactgc cgtgcccatg cctcccagaa gggccacact gaaggcaacc ctgaggatcc
    18001 cccttgtgca gattgttcga acgtcacccc tcgccatcgc gatcatgccc gttctccctg
    18061 ttgtaatgga ctccgtcgcc aggctcgttc atccagcagc tgctgatcct gccgttctcg
    18121 tcgacgacgt cgttccagcg cggcgcgcgc atcaaggagg tccagcttct tgcgatactg
    18181 catggcttcc aggagctctc cgcgtttgtc ttccattttg acctgttgat ccctctgcat
    18241 ctgctccatg ctcttccgtg ctgcgatggc ctgatccatc gcgtcgaggt gaccgtacac
    18301 ctgatccacg gtgctcccct gtcgcacctg gagcaaatat tggtcggcat ccgccctcgc
    18361 ccgcgaatcc aacatcgtca tacggtcgac cgtctgctgc agcgcgctcg cgagttccgc
    18421 caactcgagc ttggcgacct cttcaagttg aatcgcgtag gtgcggagca cggtcacgtt
    18481 cattgaatcg ttcccgccag cgccagcaag gcatcgatgg actgcgacaa cccgacggac
    18541 tgcttcgtct cctgccgcag aaacgccgtg atgccttcat gagcggacac ggctttatcc
    18601 agctccttgc tcgtgcccgg cttatacgcg cccagtgtga tgagatcttc agatcgccga
    18661 tagagcgccg tgcgttccag gaccaatcgt gccgcggcat agtgtgccgg cgtgacaatg
    18721 tcgcgcatga cccggctggt actctgcaga atatcgatgg ccggaaagtg attgcgcgcg
    18781 gcgagttcac gcgagagcac aatgtgtccg tcgagaatgg agcgtacggc atccgcgacc
    18841 ggatcattga ggtcgtcacc atcgaccagc acggtataga gcccggtaat gctgcccccg
    18901 ctttgtgccg gtccgacccg ttccaacaac ttcggcaaca cggcaaagac ggacggtgta
    18961 taccctttgg tggtgggagg ttcaccgatg gcgagcccga cttccctctg gctataggcc
    19021 aggcgggaaa gggaatccat catcagcaac acatgtttgc cgcaatcgcg aaaatattcg
    19081 gcgatagccg tcgccactaa ggcgccgcga atgcgcacca gcggcggttg atccgaagtc
    19141 gcgactacaa cgatggagcg ggcccgtgct tccggtgtca ggtcgcgctc caggaactct
    19201 ttgacctcgc ggccccgttc gccgacgagg gcgatgaccc tgacgtccgc ctgcgtatac
    19261 cggctgatca tgcccaacaa gacacttttt cccaccccgg agccggcgaa cacgccgatt
    19321 ttctggcctt ggccgcaggt caacagggcg ttgatcgccc gaatgccaag atcgaccggc
    19381 tgggtaattc tggcgcgatg caatggattc agcggaggcg catacaatgg aacctggatg
    19441 tcggtgcgca ccggcggcaa tccgtccagc gggtgcccga gtccatcgaa aatgcgcccc
    19501 aacatctgcg gtccgaccga caccgaagcc acgtgccctt tcatcacgac acggctgccc
    19561 ggtccgatgc cctgcatttc acccagcggc atcaacagca cacgatcctc ccggaatccg
    19621 accacttctg ccatcacggc gccatgacca ccttcgcgag tgatctcgca gacctctccg
    19681 acagaggtga acggtcccga ggcctcaatg acgatcccga cggcttgggc cacccggccc
    19741 gtgacggaca agggttcgac ttgttcgaga cgttcgctaa gcttcaccgg tatcccgctt
    19801 tctcaaggct tcaccgagcc gcagcagttg ggtatccagc gtggcgtcga tcaagaggct
    19861 ctgcgcctgc acgagacagc cccccgggct aataccggga tccgtttcaa atttcagggt
    19921 caatagccca tgcggagtct gactcaatgc cgcccgcgac gcttcgagca gcggcagatc
    19981 cgacggatgc acccggatcc ggacaagccc gctttcatgc aaatgggcca gtgccgtccg
    20041 tacctgcgtc acgatcactt cacgcttttc cgtcaccagt tcgcgcagga ccttctgcgc
    20101 gatcatgaac gaaagtgatg ccacttcttc ttccaccgtg tcgtgcaggt ttcgccagct
    20161 tccctccaat tgcgccatca gcgaggccac gagcgcctgc tcccgttgtc gcagcgcggc
    20221 gacccgctcc gtgacccgct tttccccctc cgtcacgccc cgctcgaact cggtcacgtc
    20281 atccgttccg aagggccgat tgccacggct gaaatcggcc agcggcaccg tccgcaactg
    20341 gatgttgccg gaacgaatca aggtgcgggt gaggaccgta ttctcctgcc tgagtcggta
    20401 cagctccagc agtctcgaca cggactgcct caccaattcg acttcgaacg gcttggttaa
    20461 gaagtccgcg gcgccggatt tcatggcacg cacagccagt tcgatattgc cgtgtccggt
    20521 catgacgata ctcagcagac ggctatcgac ctccaaggcc tgttgcaaaa aggcaatccc
    20581 atccgttcca gccaattgaa catcgacgat cagcacggca atcgggcggg acttgaggtg
    20641 cgtcagggcc tcgctgacag acccggccgt gagaatcggg tgccctgtgg actgcagcat
    20701 ttcggtcaag aggaagccga tggatggttc gtcgtcaacg atgagaatgg cccgtggggc
    20761 gagttccttc tcattgtccg cgggagacat ctgtggcagg cgacttccca catcaacggt
    20821 tctcatctac agcaactcct cgcctccccc gcccccgatc acgatgcgac cttcctcctc
    20881 aagcttgcga cagactttga gaatgttctg ctgcgccttc tcgacatcgg ataccttgac
    20941 cggcccgcgc gtttccatgt cttccttgag catttcggcg gcgcgactcg acatattctt
    21001 gagaaacttc tccttgatct ccggcgtggc gccacgaagc gcgaccggga ggtcttcttt
    21061 gctgatttcc ttcatgagtt cctgcatgcc gcgggaatcc agttccacca ggtcatcgaa
    21121 gacgaacatc agcgcccgga tgctttctgc cagggcctga tcgcgttcgg tgatcttggc
    21181 cataatggcc ccttcggtgt tcttatccac acgcgtcaga atatcggcca tcagctcggc
    21241 gccgccgacc gacatgctcg acatgcccat cgaagccgac agaatttcct gcaaactatc
    21301 gctcaattcg gacagcactt ccggctgcac ttcttgcatt gtcgccaatc gcagcgacac
    21361 gtcggcatgc agatgtaccg gcaaaaacgc cagcacggca ctggcctgtt cggcttccat
    21421 ttgtcccagg cagacggcga tcgtctgggg atgttcgatc ttgagaattt gcgcgacggt
    21481 tcggggatcg acccacttca gggcgtcgat acctggatag gtcttcgtat tcaaggactc
    21541 catcattctg gcagcctttt caggacccag cgccttcttc agaatggctt ccatgaattc
    21601 gcgtccctcg aattgcacct cgccggatga gctcgcctgc tcaaattcgg cgatcacgct
    21661 gtcctcttcc tgtttcgtaa tgtttgcggt ctctttcatg aagctcccga gcttgcgaat
    21721 atccttcggg tcgagggtct tcatcaccgc cgccgccgct tcctcaccga tggctcgaag
    21781 taggatcgcc gctttttgtg cgcccgacag ttctttattc atgatctgct tggttaggcg
    21841 ttactcttca accactgttt cacgacgacg gcggtattgg cggggttgtt cttggccata
    21901 tcgaggatct gccccggctg cttgccgctg atggccgctt caacctgtcc caccgacgcg
    21961 ggcagcgccg gcgtctcgcc gccgccggct gcgggcgcgg actgaaccag catcaccatc
    22021 aacgggcgaa ccaccatgaa gaaaatcagg aaaaacagaa caccgccgac cgcgtagcgc
    22081 acgtaaggca tccaggcctt gctcgcatcc ggcgtagcct cgaccccacc ggccggttcc
    22141 tccgctccta gcccgaactg aatgctcacc acttccactt gatcctgccg ttcggtcgaa
    22201 taccccatcg ccttcttgac gatttcttcg atgcgtttca tttcttcctc agaacgaggc
    22261 acatatttct tgggctgatc cgcaccgcct tcgcccgcct ttcccccgcc ctcatacgtg
    22321 ccgtctacca ggactgcgac cgagagcttc ttgatggtgc cggtcggctc aacgatgcgc
    22381 gacacggttc ggctgatttc ataattgacc gtctcatttt tcgtctggtt gttgttcgag
    22441 ctggtttgtc cgccttcggc ttcggtgcca cccggcacgt tcgactcgac gccgggcacg
    22501 ccgcccgagg tgccgttcac cccgctcgat ttttcctgtc cgcgctgctc actgcgaacg
    22561 acctgcccgt tcggatcgta gcgttcttcg gtggtttcga ttttgcggaa atccagcaca
    22621 ctcgacacgc gcaccacggc cttattgacg ccgacaatcc gttccaacat cgtttgaata
    22681 cgggtctcga tgtccttttc cagcgtacgc tgatattcca tctgcgttcc ggagagcccg
    22741 gctgattcat cgctggaggt gttggacaat aaattgccgt ggccatcgac gacggtcaca
    22801 tcgcgagctt gaagcccttc gacgctgctg gacaccaggt gcaccacgcc ctgaatttgc
    22861 gccttcgaca acgtttgact ggcgcgcagg gataccacca cagaggctcg cgcacggtcc
    22921 tgctcggtgg cgaagagccg gcgttcagga atcgccaaat gcacccgtgc tcgttccacc
    22981 tccggcattt gagtgatggt gcgggccaac tcgccctgca aggcccgtcg ataattcaac
    23041 ttctgcacga aatcggacat gcccatggtc gtccgatcga aaatctcata tcccacgccc
    23101 ccgccatgcg ggagaccttg ccccgccatc tccaaacgca agtcatgcac ctgcgcgctg
    23161 ggcaccagca cggtggtccc tccgttggcc gtctcgtagg gcaccttcgc atctttcagt
    23221 ttgtcgatga tgcctgacgc atcatcgacc gccaggttgg caaagagcac ctgcatatcc
    23281 ggctgttggg tccagagggt taccgcgacc agtcctgcaa tggacccggc gagcgcgagc
    23341 aggatgatga agcgttggtt gatcgtgaac tggctgaatt ttgaaaacat acggtgtgga
    23401 atcctttatg cgtgagaccg gcgaccggac aacaaccgcc taaatctgca tccgctgaat
    23461 ttcctcgtag gcggtgacca gcttgttccg cacctgcatc atcaactgaa aagacacatc
    23521 ggcctgttgc aatgccacca tggtttgatg aatgttggtg ctctgcccgg tcaccaacgc
    23581 atcgacggcc tggctggcgc cggtctgtgc atcgttgata tgcccgatgg cctccttgag
    23641 ggagcccatg aagttcgctg cgccggcctc accacctggc cccgcctcgt gaatctcagg
    23701 catctcaatc ggtcggaagc cattcgctcc cgcaatacgc agatcagaca tgactacctc
    23761 ccgatctcca acgcacgatt ccacatggtg cgggtcgcgt taatcgcctg cacgttcgct
    23821 tcataggcgc gcgaggcgcc gatcatgttg accatctctt ccatgacatt cacattgggc
    23881 atcgtcacga atcccttttt atccgcatcg ggatgacgcg ggtcatacac cgtctgtccg
    23941 ggcttcttgt cttcgatgac gcgagcgact ttgacaccgt ccaacgcttg acggcctggt
    24001 ccggacgtca cttgcttgaa tgccttttga aacgccgagg atacgggcgc ggcctgaaac
    24061 acgacatcac ggcggcgata gggtcccccg gagctcgttt tcgtcgactg ggcgttggcc
    24121 aggttgctgg caatgacatt gagtcgatgc cgatgggcat ccagcgcgga gacggagacc
    24181 gcaaggctat ctgtcaattc catcacacac ctctttgtgg ttgcttccgg aagtcatgag
    24241 ttgagtgttt cccacgatcg cgcctaccgt ccttctcgaa tggcacccaa aagacctttg
    24301 aacttcatcc cgatgatggt cgctgcggtg ttgtattgct gagcgttatc cgacattttg
    24361 gccatttcca attcaatgtt gacggaattg gcgtccagcg gcagatcgcc tgccggcacg
    24421 tcttcgagct ggccgtggac ccgttgataa ccagtgcctt gtggcccgaa atgttgagcg
    24481 ttggtggaaa ccagagagat cggcaacttg ccctgctgtg cactggccag tgcctgaccg
    24541 aaattcaaat ccttcgcgcg atacttgggc gtttcttcgt tggcgatatt cgccgcgatc
    24601 acctgctggc gagcaccgcg aagatccagc gaccgactca acaactgcat ggttctgtca
    24661 aaaatagtca tgtccgacgc tccttagaaa agcttcacca tcgcaggatg tgcaatacgt
    24721 ataccgcgct tgcggctcat cgaatcctga ccgcgtcctt ttttggtgcg ccgcttaggt
    24781 cggcacccgg caaaaatgtc cacctcggag aataatctgc ccatctccct gcaagcgaca
    24841 ttaggacttc tcggcctcga cctggtaccc catgtcgcga tactcccgca atttattccg
    24901 tagcgttctg atactgatcc ccaattcctt cgccgcatgc gtgcggtttt ctttcacgcg
    24961 ggccagggtc ttgaaaataa gatcccgttc catctcccat agcgacccat gtgcggaggg
    25021 cgtcacgatc gcgtcgccga cctctacctc ccgtaccggc accgtgccat cgcccggcat
    25081 cagatgatcc acatccaccg cccctccggt ggctaccagg atggcacgct ccatgacatt
    25141 ttcgagttca cgaacgttgc ctttccaggg acgctgctgg agatcggcga tcgcccgctc
    25201 ggacaacgtc ggcatggtga gcccgtttcg ctgtgccgag gagcgaagga aatgtctcgc
    25261 cagaagcgga atatcgcccg accgatcacg aagcggaggc accgtgacgg gaaacacgtt
    25321 cagccgatag aaaagatcct cacggaatcg gccctgggtg acttcgtgat acagggagcg
    25381 attggtcgtg gcgatgacgc gaatattcac tggaacaggc tcgcgcccac cgatacgatc
    25441 gacttcacgc tcttgcaaga cgcgcaaaag tttggactgc aagcctaaat tcatttcgct
    25501 gatttcatcg agaaacagcg tcccggtatg cgccatttcg aacttgccta actttttctg
    25561 aatggctccg gtaaatgcgc cgcgttcgtg gccgaacaac tcactctcga ggagactgtc
    25621 cgggagtgcg gcgcaattca acgccacgaa agggcgatgc gcgcgcggac tgcgcgcgtg
    25681 gatatagcgt gccaggagtt ccttcccggt cccgctctcg ccgctgatca gcacggtcgc
    25741 ctgactcgcc gcgacaccct ccagcgtggt gagcaggcgg atcatgcccg ggtcctgcgt
    25801 caaaatcgcc cgcgcttcca gtgtggcggg cccttcctgc tcgtccggcg ccgtggtggc
    25861 ctgcaggttg agaatcactc gttccaaaag gtccgtcgag aacggcttta agatgtagtc
    25921 attggcgccg tatttcatcg cttccaccgc tgtctccacc gtgccgtagg cggtcatcag
    25981 gacaatgaac gtctgggggg atttttgttt gatggccttg acgagttcca gcccggtcag
    26041 gcgcggcatc tttaaatcgg tcaccaccag ccagggtttc actcgttcaa tctgctcaat
    26101 tgcgtcgacg ccgtcgatcg ccccccggac ttgatagcct aatctgcgga cggtttcaga
    26161 taaggccgtt ctcatcgagg gttcatcatc gacgatcagg accatccgag cttcttcgga
    26221 agtggcggag gggccttgta aatcctgttg cacgctcatt cgtaggtctc ctcttcggtt
    26281 gcattctcga ttgggcgggt ttgcacggac cggccgacca acgcggcgtt tcgcgcagac
    26341 agcgttggct gttgtatgga accctggggt aatctcatga caaatgaggt cccctgcccc
    26401 ggcctgctct ccacatcgat gcggccgtga tgagcctcga ccagcgcatg tacgatggcg
    26461 agccccaatc ccgtcccatg gtctttcgtg gtaaaaaacg gatcgaatac ccgcgactgc
    26521 agctcgagcg gaatccccgt gccggtgtca ctgaccgtga gttggatggc aggcaattct
    26581 gagatttctt ccggcgccat acgagcagac aatatcaggc tgccgccttc gggcatggcc
    26641 tggacggcgt tcagaacaag attcagcagc acctgtttta ttttcccgcc gtcacaccaa
    26701 attttcggaa cgcggggatc gacctcgcaa cggaccacga tcgaagccgg tgacatggca
    26761 tggctcgcca gcgtaagtac ttcccgaatc agcgattccg tatcctgcca tgccaacctc
    26821 gaacagtccg gtctggtata gacgagcagg ttcgacaaca accggtccat ggcctgcacc
    26881 gcgacggaga tatgctccgc gtatgttcgc agatgcggct ggtcacgcag atccttgcgc
    26941 agcatcgacg cgaacaattc aacgctgccc aggggattcc ggatctcatg cgcgatgtta
    27001 ccgaccatct gccccatggc ctccagccgg ttccgccgct gcaaacgatc ttcaagctgt
    27061 cgaatgtggg tcacgtcgtg gatcaacacc agtttgccga tcaaactccc ggtttcatcg
    27121 gtcaagtcgc tctgcgagag ggcaatggtc acgcccgtcg gaagcaccag cgggtactcg
    27181 ccgtggtctt tccggatttc ctccagcacc tctgtcgctc gacgccccct gaggcgggct
    27241 tgcggaaccc ccaacagacg ttccgtggcg tgattgcacc gcaccaccga gtcctgcgag
    27301 tccgcgacga tcacacccgt atccaacgat tccaacaccg ctgtcacatg ggcgcgcatt
    27361 tcttctgtct cacgtagatg ttcgcgcaaa ctggcattgg tttgcgcgag ttcgagatcc
    27421 atctgctgca gccgtgtcgt caacgcgtca tacgactgtt gaaggacggt ggcagcctgg
    27481 tcaaagtcat ggaacgcacg tgtcagcaga tcgttgttcg atgcttcggc atcgcgcgct
    27541 gtcatcgctt tttgcctcct gcggcggaag tgggctcagg cagttcggtc tgcagcacgg
    27601 cggccatacg ccgaaccagg ctatcgctgt tctcgttcag tgcgcgaagc ccgttccttg
    27661 cgaactcttc acgcttcacc ccccgcgcca actgcacgat ttgaaattgg gcccaggctt
    27721 cctgttcagg ctccaacccc gcaatcaatg cctgcttata cagggccagc gccggctcct
    27781 gacgattcag tcttgccaaa acatcggcat atcgcagccg gtccaatgcg gacaggtcca
    27841 tccccgcctt gagtacgctc tcatagaggg gagcggcttc ggcatcccgt tttacctgcc
    27901 tcaacgcatc ggctactttc agctgaacaa atgcgcggtc cggatggcgc gggttgtgta
    27961 tgagccactg ctttccaagc ttcaccaagc cgggcgtatt tccttcgccc tcgaaggacg
    28021 tcaggattcc aagcagagcc tgtccagcga accggccgac gggaaattgc aaccgatacc
    28081 gctcgaagac ggcccgggcc gcgcgcatgt ctttttgttc caagtaacta tcgccgagcc
    28141 ccattaaggc gacttcgtga aaggcctcgg ctttcgagtc tcgaatcaac gactgaaaga
    28201 gccgagccga ctctattgga aatccaagcc gacgatgcgc ctcggccacc ttgagcagca
    28261 gttccgtacc ggcgtacagc cgatcggcaa acgggccgtt acggtggaac aggttgatga
    28321 gctcaaaatc gtcgtctgct ttgagtgccg cttccagccg ggggcgcaga aatctcacga
    28381 gctgcgcccc acccttctcc ggccagggat cgttctcaaa tttccctgcc cgctggacca
    28441 cctgctcgta ggctttcagc gcgtcttccc gctttcctgc ccgctccaac gcctccccga
    28501 gatgaaataa cgcctcgctt ccaaccggtg agtcttcata ttgcttggcg ctgctttcga
    28561 acaactggcg cgggctgagc atttcaccgg gcttgagggg aacattcgcg agatgtgcgg
    28621 caatcgtgtg ccgaagattg acctcctcgc cttcgggagc gagatgctcc tgcacctccg
    28681 catatcggag cttggccgtg ggcacaacct gcgcatccgg atactggctc aacaacgccg
    28741 cataaaaaat gctcgcatct tcccatcgcc cggcctcttt gtaactgtcg gccaggtgca
    28801 tcaacacaaa tggattctcc ggcctggagg gatacaggtt gtagaaatgg agaagctgtt
    28861 cccgcatgac cggtccgcgg tgggcttctc cggctgtatc cgcgtaacgc agcaatgcaa
    28921 acgggtccgc ccgaagagcg ccaggccagc ggctgctgat gctttcatac aacgcatcag
    28981 cgtccttgat ccgtcccatt cgatagaggc tgtgggcctg ccccagcgac gcggacacca
    29041 gcagcgtggg gtccgtggtt cgtttcagga cgtgatcaaa cgtctgtacg ctgtccttcc
    29101 aactcttgat gcctcgaaag acatagccca gaccgagcat agctcgattg gcatcgtagg
    29161 aatctcgctc ggagagactg agcgcatgct ggtaggcgat ctgcgcctcc tggtaccaac
    29221 cttcggcacg atacacatcg ccgattctcc aggcggcccg cctggcattt gtcgactgag
    29281 gatcttctcg aatcagggcc ttgtactgat cgatgatttc cagaggcctc agttcttgtg
    29341 cgctactctt taaggagctt tctgttagaa atgcttgaat agacgagcgg agcggaatat
    29401 cgcgatgctc tctcagcatg ttagtcaggt gaagtcgcgc ctcgagatac tgcccctgat
    29461 tgtagagggc cactccccgt tcaaactcac cccattcagg actctcggga atccgttcca
    29521 agcgaggccc tggatccgga agtgcgcctc gaaccaattc cgacggctca ggcatcatgg
    29581 caggtaattg ctgtcgcatc gtcgaggatc caccggcagc cataacagaa gagcccccag
    29641 cgagcaacag actacccagg aaaaatgcca gacctatacg gatcctgacg gattgttgtg
    29701 gcgaagatcg gtattgcaca gggggcaaat cagcaagtcc tgtgcccttt ctggaatcat
    29761 ccaaatagcg gcatcacttg aggatttcgg tcaacagcga aaactggaca gcaggtgttg
    29821 tcagaaatcc cgacaggcac tttgtggggc cggtcaaaaa aatgacgtgt gagaggggag
    29881 aacgtgagag gaacgttagt tttgaaccga atacccgtga gtcttgggat caaatccttt
    29941 ccgtttgagc ttttccacca gtgtggttcg attcacttga agcaactgtg cggctcggct
    30001 ggtaattcca ttggcctttc gcaacgcttc cccaataagc ctattttcat agtgttcgag
    30061 ttctttggtc aaattgatcc cgtcttccga aaagcgaata aactgctcag gcgactcgac
    30121 tcccttcgcg gcattcattt tgacgggccg ctcaggcaaa tcgcccatag tgatcatgcc
    30181 ccgtttcttc aacacacaga gacgttcgat catgttttcg agttctcgga tattccctgg
    30241 ccattcttct tccatcatgc gggctaacgc atcaggctcc atgcctagaa tctctgtccg
    30301 ccgtaattga ttaaactggg cgatgaaatg gttgaccaaa agcggaatat cgctacggcg
    30361 ctcccgcaat ggcggaatgt gaatgggaat aacatgcagc cggtaataca agtcttgtcg
    30421 gaaccggcgc tcttgaaccg cttgcgccaa atcttgattc gtcgccgcaa tgatgcggac
    30481 atcgacattg attgtccttg tcccgcccac gcgctcaaag caccgctctt gtaacacccg
    30541 cagcagtttg acctgcaacg gcaaactcat ctccccgacc tcgtccaaaa agatggttcc
    30601 gccatgagcc aactcaaatc gcccgaggcg cgtatgggcc gctccggtaa aggccccctt
    30661 ctcatgcccg aacagttcgg actctaacaa ggtttctgga atggccccgc agttgaccgg
    30721 gaccagcgga cgctccctcc gcatgctgtt gaaatgtaac atccgcgcga tcaattcctt
    30781 gccggtccca ctctcacctt caatcagcac tgtgctgtcg ctatcggcca ccttttcgac
    30841 aaaatcaagt accatgcgca tcggtgcgct cgtccccact agatgctcca ggcgatactg
    30901 atctcgcacg gcttttcgaa gcagatggtt ttcctgcttc aacttgtaga cgtccaacgc
    30961 ttttcggacc actaccgcca cggtatcggg ctcaaagggc ttggtaataa aatcaaatgc
    31021 gccggctttc atcgcccgta ccgcgtagtc gactgtccca taacccgtca tgacaatcgc
    31081 aataatcttg gagtcgattt ttgagattcg atcaatggtt tccaacccat cgatccccgg
    31141 catctggaga tccgtcagca ccacatgtac cggctgatcc ttgacagcct ctactccgtc
    31201 cagcccactg gacatgaccg tcacctgatg tccttcacca gcgagcgtct ccgccaagat
    31261 ttgacgcaca gcaggatcat catcaatcac gagaatatgt gactggctca tcaagggcac
    31321 tcctctctag aggggaccaa agaaatcagg ttcggctaaa taaggatgac cagaacgcac
    31381 tacgagaaac aactctctcg ttgaatggtt gaacacagaa tcagcttatt gggaattgaa
    31441 actttattca agtgaaagta aggatggcct gcatagctga gccatcagat cctacacaaa
    31501 gcgagcttct ctctccgatc gcacccagcc tagatcttga caggtgagaa gaagaattgt
    31561 agggttcgaa atcttccttt cagattcact tggtattaat caggtagctg atctcgcgag
    31621 ctggcgtagc tcaattggca gagcagcggt tttgtaaacc gccggttggg ggttcgattc
    31681 ctctcgccag ctccacaaat cagacggacg tgtcaagcac gtggcggctc gcgaactcac
    31741 tcctcgtatc acgcggttct ttaattttac ccactgtagt tcaaattagt ccggaaggta
    31801 cgcctgaaga accttcaaac cctcgttgct tcacaacagt gtggcaaccg tatacacgac
    31861 gagctctgct gctagaatgg tgcgcgacga gcctttccag cactccggct cgcacttggg
    31921 tattatgcag tcactactat tgaaaccagt aggtattttc acttccacaa ttcgatgccc
    31981 ccgtactatg agctattctg cgtagccgga gagcctcaga gtcccataag catgagcttc
    32041 ttgcatcaat gcagaattga catctcaacc ggttccccag gtctacagag actaatagaa
    32101 gggctctgat caagcatgga taactaaaca acgatccccc ctaaagagtt accgattctg
    32161 aggactaggc ctcccaccaa cctcagcata tagttgagaa caagagcgac tcaactcacc
    32221 cgtgctggtc ccagtgagag acaaaggagg cgcgatgaaa aatcgatata gccaacgagt
    32281 atcagtagat tgttccgtca tgtttgccgg ggaaaacgtt gttgccgaag ggcggattct
    32341 cgacctctcc ctcccaggat gcctcatgga aagctcgaaa aagatgtcca ccggtgaata
    32401 tatccaactt cggttatttc tgtcggacca ccaggctccg ctacatgtcg cacttgccgc
    32461 catcagatgg gccgaaggct cgaaggttgg catcgagttc atccgcacat ctcaatctga
    32521 acaacatcga cttgaacaat tcgtgcggcg gagttccgga tgcgatgctt catcgacatt
    32581 gagcgatagg ggtgtccttg ctggaacagc gtggaatgag aaacagtcgt agctgtttcc
    32641 gcagattgat ccccagggaa gtacttgcca tgccgaaaca cttcaaactc cgtacttacc
    32701 agagagccat gatttgcgga acggcatact atctctcgga agattttctg gggaaaggca
    32761 ccgtctggga tatgtcatcg ggaggatggc gtatccaagg agaccatcaa gtcaagatcg
    32821 gcatgctact cacactgcga atggagctgc ctgaggagaa aatgccgttg gaaatcgagc
    32881 atgctgttgt tcagtgggtc agcggagagg actttggagt ccgtatcaag aaaatccgtc
    32941 ggtcggcggc cattaaacta gagcgtctta tgggacacca cctgtgcata cttcctcctg
    33001 tcgagcacta gacatcctta acctcttccg tcgcttcttc cagatcagta agatcaagat
    33061 ctgcgcgttc tttcaacatg gagggatgca acccatactt cttcagcatc ttgtagaaat
    33121 ccgcccgata tctcccggcg aattgtgcag cccgcgaaat attcccgccc gtcatttgca
    33181 gcacattacg caaataggag cgttcaaact cttccttcgc ctcggttagt ggcttcaagc
    33241 cgatatctga cgccacacca gccgtaggga gtaactctgg caataccata tcttgccggg
    33301 acattacgac agccttttcg acagcatttt cgagctctcg cacattgcca ggccatggat
    33361 acaccatgag tcgatgcatg gctgcaggag taaagccacg aacatcctta ttcgaacgtt
    33421 tagcgctctg cttcaaaaaa tgctgcgcaa gaagcggaat atcgtctttc cgctcccgca
    33481 gtggggggat agctagaggg actacggata ctcggtagta gaggtcatga cggaaggttc
    33541 cggcttttac gctttctgcc aggtccttgt tcgtggctgt aatgatgcgc acgtctacct
    33601 tggttgcgta gtccgccccc acctcccgca cttctcgctc ctgtaccgca cgtagaagtt
    33661 tcacttgcat gggtaaggac atttcagcaa tctcgtcgag aaagagagtg cccccattcg
    33721 cactttggaa gagacctcgc ttggccgcga ccgcattggt gaaggcgccg cgaatatgtc
    33781 cgaataattc actttcaaag agggtctcgc tgattgccgc acaatttaag gctacaaatg
    33841 gccctttgga acgacgacta ttggcatgaa gtacgcgcgc gagcacttct ttccctgttc
    33901 cggtctctcc ggtcaacaga atggtcacat ctgaatctgc aatctgcgca acttgctgga
    33961 acaatcgttg cattgcggga cttctggcaa tcacgttctc cagtccgtat agctccttga
    34021 ctaaagattt taggcgctga atctcacgcg tcattcgtaa ctgaatcagc gccttgtcga
    34081 gggtcgcctt tagttcttta tcatcgaacg gcttcgtcag gtagccgaaa gccccgcgtt
    34141 gcatcgcctc gacggcattg ggaatgctcc catgagccgt aagaatgatg accggcaggc
    34201 ccggctggat ctgcatgagt tcttcggtca aggccaaccc atcttcagct cgcaacctca
    34261 agtcagtaat agcgatgtca aacgtttcct gccgagcagc cgtgagcgca tcgcgaccac
    34321 cggtgcacgg cgtcacagaa aacccaagcg ccgacagccg catcctcaac agatgcaaca
    34381 atccttcgtc atcatctaca acgagaatgc gttcctgctc catgactgcc cctagggttt
    34441 ggaggactcc ggctgttgag gtgaaaaaac attcgacgga ggtttgatcg ggcgggtctt
    34501 tcccctcatt tcctgatcaa tgcgcttgag ggcttctaac tgactcgtga gctcctcaat
    34561 ttttctatct cgatcactga cttgtcgttg caagctttgg agcgtagccg gctcgaccga
    34621 catccgcggg tgttctcttc tggaattgaa caattccgcc ttccgctcct ggtcttgaag
    34681 ttcccgttgc aacgcctcga gcgactgagc ctcactatcc tgcagcgccc gtaattgctg
    34741 aattgtgagt tcgcgatcaa gcaagtcgtg tacggtccgc tcaattgcct gggccaaaag
    34801 cctctgtgta tgtgcagtag ccggagcatt ccagacggaa cggatccagg actgatcggc
    34861 aggcacttct tctgactgca acaattgcag ccagaggcga ctcgacgctg ccagctgact
    34921 cttcggcgcg acagcaataa ctttctcaaa atactggctt gctgactccc gactttcata
    34981 cagagacgcc aaggcgcgcg tgaagaatac atgatcgcag gtatttttac tagcgcattt
    35041 tggcactaac tggatttctt tacgtataat ggcctgcaac gcctttccgt cgctgccatc
    35101 accataaaaa tagggtgtct ggccgggggg cggagacaca gtgctgcagg ctgacaagag
    35161 gccgagtccc agcagtactg ccatgtttcg cagatgccac actatgccag tatccccggt
    35221 tttgtcagac acaaaatgaa ccgcacagtc gttccttttc cgactttact ttcgacccag
    35281 atacgcccac catgggcttc cactaccttt ttggccaacg ccagccctaa tccactgccg
    35341 accgagacat gtcgtgtctt ggtgcggccc tgataaaagc gctcgaaaat atgcgggact
    35401 tcctccgtcg gtatacccgg cccacagtcc gtcacatcga cgaacagcaa ccccgcatca
    35461 cgccgcggct ccatgtgcaa cttcacggcc gccccctccg ggctgtactt caacgcattc
    35521 gagagcagat tgtccagcac ttgttcgatg cgagcgctat cagccctcac ccaatggcgt
    35581 tcgaccggag cctcgaccag gaatcgcact tgtttcgcat ccgccaacaa cctgatttta
    35641 ttgactgaaa tgtcagcgac tcgcctcagg tcggtcggag caaaccggta ctccatcatg
    35701 ccggactcca ttttcgacaa gtcgagaatg gtcgaaatga ggtacatcaa ccgccggcta
    35761 ctgtcggaca tgatccgcaa tgtcgtccgc tggtcctgcg tcagcgggcc gggaatttca
    35821 tccaagagaa gatgggtccc ctcctgaatc gaagccatcg gcgtacgcag ttcatgggac
    35881 acatgggcaa gaaactcgct cttgatatcg tcgagttgct gcagcctggc ccccatccac
    35941 ttcaccgtat cacccaattc tcgaagttca cggggtgccg gaccatccat tgtcgcccgg
    36001 aaattcccct gccccatttc ctgaatcgtc gactgcagtc ggcgcaacgg tcgcaggata
    36061 ttgtaacttg ccaccgcagc catacccagc ccgaacatca atgccaccaa caccaattgc
    36121 cgtgtgaccg cttccgcatg aactgagctc gcatgcgact cattcacccc cactgcgata
    36181 cgcgcctcat ggagcccgat gtactgctgt agagtcgacg ccatcttccc ggccagagcg
    36241 tctctccgtt catcataggc tgcgctcgat tccgcgggtc gcccaacgcc ctcagcaagt
    36301 tgggtgtgga aaagagccaa atgcgcctgc tggatccgct cgacttccct caccaaccgg
    36361 atctccggct cggaggtgtc ccgcatgagc aagccttgca agatcagttg aaaatctttg
    36421 ctttcggcgt cgaagtgttc gagaaacgtg gcggccggca cagccacata ctttttctcg
    36481 ctctgaactt gctcgtaaaa cgagctcagc agccgttttg ccgactcaat ttccggatga
    36541 tgatgggagc cgacctcggt attgaggccg gccaatgttc gaatttgcaa caaagcataa
    36601 atgttgacgg ctgtcatcac cgcgatgatg accacatacg tcagaatcag tcgccagaaa
    36661 atcgagaggc gcacgacggc gcatcctccc cggtgaaccg cacgagaacc agagcaatcg
    36721 gcagtgtagg ttaagccata caccacttcg tgcacaccct caacaagttc tccatattgt
    36781 gcaacgcaca gggatggatt ctggatggct ggtacgcctc ctatcgggcg gagtcgtgga
    36841 actgcgattg atacaaacgt tgtcgtctgg aggttgcgga tagggcaaca aacaggtggc
    36901 cgcgaaagat cagcatgccg accatgagcg gtggggtcag cagcagcgct atcatgccgg
    36961 tcagttccgc tgaaatcccg aaatctgtca gccaggcgct gagcacggta aacggcacaa
    37021 acaccagttg aatgaaatcc aacccggccc cccgccccag ctgactggat agtttgaaaa
    37081 agagggagag tccaagaggc gccagtacca acgccatggg caggaaccat tcgatccagt
    37141 tatcgagcac gaaatcgtaa ctccgcttca aaacatcaag gggcgaatcg tgccgggtga
    37201 gataaatgac ttccggggct ggattcagaa ggataaacac gagcagcaag caggccgtga
    37261 ctagaaactg gctgtcaggg ttcgcgcgca ggctcgtatc cagcagcaag gtgggaatcc
    37321 acaacacgaa cccgacgctt atcacgtccc agaaatagtg ccccatactc tcgatcacgt
    37381 cgccgagggt aatggcccgc atacccttaa tcgcctgctc gatcaagctg aggatagccc
    37441 cgatcaagca ggcattgacc gcacccagca gaaacccgcc cagcatcccc agggggcggg
    37501 cgatctggtg agccaacaac atgagcagcg taaatacaat cacggcgggt atcgccagcc
    37561 atccacggac cagggaacga ctggtggcgt gcagggcctc ccgatagaga tgcagggtgg
    37621 aggtcacgac agtcagcatg gatcaatcga aaggaggtag atgttcatct cagaatgcct
    37681 ccgcctggag aaccctgttc catctatcgg aggcggacaa taagccggat acacccgaca
    37741 ggtcaagtcg atcagcactg cccttggatc tggaagtgtc gcgagataca gccgggccac
    37801 gcgctattga cttgcgcgtc gatatgatta tgttctttca ctagatacac ccacccagga
    37861 gtgagccaca tggatatgaa caggatgacc atcaagctac aagaggcctt gcaaagcgca
    37921 tcagccctcg ccatgcgcag ggggcaccag ggcattgatg tcgaacacct gctccttgcc
    37981 ctgatggagc aagaaggagg gattgcggga tccttgcttg aacaggccgg ggtgtctcct
    38041 gccgccgttc gccaagcagc agagcaggcc ttgaccaagg tggctcaagt gcatggcccc
    38101 ggcgcggctc ccgggcagat tcatctcact ccccgcctgg gctcgctctt gactaaagct
    38161 gaagaccgga tgaaggaact ccgtgacgag ttcgtgagtg ttgagcacat tatcctggcc
    38221 atggtggaag aaggtggaat ctttcgccgg ctcaatctca cacacgaacg cctgctgacg
    38281 gccttgcagc aggtacgagg aaatcaacga gtcaccagcc aggacccgga aggcacgtac
    38341 caggcgctcg aaaaatacgg gcgggacctc acgaaactcg ccgggcaagg caagctggat
    38401 ccggtcatcg gccgagatga cgaaatccgg cggaccatcc agattctctc acgtcgcacc
    38461 aagaacaatc ccgtcctcat cggtgagccg ggtgtcggca aaacggccat cgtggaggga
    38521 ctcgcacaac gcatcgtcaa gggcgacgta ccggaggggc tcaagcaaaa gcgcgtggtc
    38581 gtcctcgata tgggcgcgct cgtcgcgggg gccaagtttc gcggcgagtt cgaagaacga
    38641 ttaaaagccg tcctcaagga aattcagtcg gcgcaggggc aagtcttgct gttcatcgac
    38701 gagctccaca cggtggtggg agccggcgct gcggaaggtg ccatggatgc cgcgaatcta
    38761 ctcaagccta tgctggccag aggcgagatg caccttattg gcgccaccac cctcgacgag
    38821 tatcgtaaac acatcgagaa agacgccgcg ttggaacgcc gctttcaaac ggtcctcgtg
    38881 gatcaaccga ccgtcgaaga taccatttca atcctccgcg gcttgaaaga acgctacgaa
    38941 gtgcaccacg gggtgcggat caaagacgcg gcgctggttg cagcagccaa attatcgaac
    39001 cgctatatcg gtgaccggtt cctgcctgat aaggccatcg acctggtcga cgagtctgcc
    39061 gcacgccttc gaacggagat cgacagcttg ccggccgagt tggacgaagt ctcgcgcaag
    39121 gtgcttcagc tggagatcga acgggaagcg ctgaaaaagg aaacggatgc gggcagcaag
    39181 gcacggctgc aatcgattga aaaagaactg accgagaaaa accgtgatct ccaggcgctc
    39241 aagacacgct gggagtccga gaaaaactcg gtcagtaagc tcagaaaaat ccgtcagcag
    39301 atcgaagaag tgaaacaaaa aatcgagcag ttcgaacgag cgtacgacct gaataaggtc
    39361 gctgagctgc gctacggcga gttgccacgg ctcgaacgag agcttgaatt ggaacagcaa
    39421 cacctcggca agaaacaaaa tgaaagccgc ctgctcaaag aggaagtgga tgaggaggat
    39481 attgccgccg tcgtcagtcg gtggaccggc attcccgtta cccgcctggt cgaaggcgaa
    39541 atggaaaaac tccttaagct ggacgaactc ctgcaccggc gcgtggtggg gcaggaacag
    39601 gccgtcactg cagtggcaga cgccgtactc cgcgcacgct ccggcatcaa ggatcccaat
    39661 cgccccatcg gatcgttttt gttcctcggg cccaccggtg tcggtaaaac agaactggcg
    39721 cgggcgcttt cggtcacgct gttcgatgat gagtcgaacc tcatccgcat cgacatgtca
    39781 gagtatatgg agaagcacac ggtcgcgcga ctgatcggcg cccctcccgg ctatgtcggt
    39841 tatgaagaag gcggccagct caccgaagcc gtgcgacgac atccgttctc cgtgattctg
    39901 ttcgacgaaa tcgaaaaggc gcaccatgac gtcttcaata tcctcttaca ggtactcgac
    39961 gatgggcggc tgaccgattc ccagggccga accgtggact ttaagaacac cgtgctgatc
    40021 atgacgtcga acatcgggag ccagcacatc ctggaagcac aacaagccgg ggcctcctat
    40081 gaaacgatga aagctcaagt gaccggcgaa ctacgggcac acttcaggcc cgaattttta
    40141 aatcgggtcg atgaaatcgt ggtcttccac gccttgggga acgaacacct gacgaagatc
    40201 gtggacatcc agctggagcg gttacgtgcc agactggtgg agcggcggat taccctgacg
    40261 gtgacgccgg acgcgctgca gaacctcggg cggcgcggat atgatcccgt gtatggggcg
    40321 aggccgctga agcggctcat tcagcaggaa attgaaaccc ctatggcgcg ccagctgatc
    40381 aaaggcgagc tacgggatgg agatacggcc gtggtggatc tgaaggacgg gcacattgcc
    40441 atcgtaccga cggtcgcgcc atagatgacg accgcacaag tgtagagggg cggaacactc
    40501 ggacggcgct atccgatgaa caccgcccct ccctttgtgt ccgtgtcttt gccactgaag
    40561 cgtttcttat cgatccgcca ttcggtttgc gacacgtcca ggcgatgccc cttgatcgtg
    40621 tggaaggaac cccggctgaa cacaacccgg tttccctctt ttaactccaa cgtaaagacg
    40681 accgtcccgg actcttcgtg cttcagcgac acactcgtcg gactcttggc gagctgatag
    40741 gcgcgccgtt cattgaaggt cagattgcta tcgaccactt tcgccatcat gcggcccacg
    40801 tcgtcgaaca gcgcgaagtt cacgagtaat gcccgcgccg gttccttgat ggccacttca
    40861 aattgcggca ccccttcgat atcgatgatg ccgttggaat tccgatacag attggaaccg
    40921 acttcaatat ccatcgccgc gactccttat cctgcacggc accgagaggt gcccgttatg
    40981 ccttcttgat acggtccaaa atcaacgcga tacagccgcc taacaatcct ccgcccagaa
    41041 tcgtcgtcaa caactcgacc agcttgagct cccacaccga tccttgagcc tggagcgctt
    41101 cgcggatccc gccctgcagc agaatcaccg ccaacgccat ggtcgcaccg acaatgaacc
    41161 accaggcgaa gactcgcgcc gtatggatga caggagattc cttcacaact cgactcgtcg
    41221 atcaaaactc cggtactgaa tcgcttcggc cagatgtgcg ggaccgatac tatcagactc
    41281 ggccaggtcg gcaatagtac gcgccacacg aaggatgcgt ccatgggcac gggcagaaaa
    41341 ccctaaccgg gtcatggcct gctccagcag ctcacggctc tgcatatcca atgcacaata
    41401 ctgcttcagg tggcgcggtt tcaattgggc attggtgtaa atcccatcgc cacgataccg
    41461 ttccgcctgg cgcgcccgcg cagccattac cctcgagcgg atcacagcgg aagaatcgct
    41521 cgtggccacg tcgtcgccaa gcgcgcgaat cggcacagcc ggcacttcga tctgcagatc
    41581 aagacgatcc agcaacgggc cggacaagcg cccccggtac cgccgcactt gcgccgcact
    41641 acagacacag tccttagtcc gatctccata gtagccacag ggacaggggt tcattgcagc
    41701 caccagcatg aaccgggcag gaaatcgcag ggaaccactg gcgcgcgtca cggtcacatg
    41761 cccatcttca agtggttgcc tcaggccgtc cagcgtcgca cgcccgaact cgccggcctc
    41821 atccagaaac agcaccccgt tgtgggccaa cgagacttct cccggtcgtg gtatcgttcc
    41881 gccaccgatc aggccagcct cggaaatact gtgatgtggg gcccgaaacg gccgccgccg
    41941 cataagaggc tgctctttgg acaattgccc gaccacgcta tgaatgcgac tggtctccag
    42001 cgcctcctcc tgagccaaca aaggcaagat tcccggcagg cgacgagcca gcatcgtctt
    42061 gcctgcgccc ggaggtccca tcatgagcag gttatgtccg ccggctgcag ccacctcgat
    42121 ggcccgtttc gcatgtgcct gccccttcac gtcggcgaaa tcttcatctt caggaagtcc
    42181 ggaagaggcc gaaccatcct gcgacaccga caccttcgag atcgtcaacg cgccgcgcaa
    42241 gaattccacg gcctgcggca acgtatgaat cggaaacact tctgtgccct cgactagagc
    42301 cgcctccccg gcgttctcgg cagagaccag caccggatga cgacggcgac agacggcacc
    42361 gatcgaaagc gcgccgggga taggtttcag gcgaccgtcc agcgagagtt cacctacaaa
    42421 cacaaaatcc ttcaccgctt caggcggaat gatatcttct gccgccagaa tgcccagggc
    42481 aatggccaag tccaggccgg caccttcctt cttgatattg gcgggcgcca gattgaccgt
    42541 gacttttttc acaggaaagt gaaagccgct atttttgagc gcggcacgaa cccggtcacg
    42601 actctcacgc accgtggcat cgggcagtcc tacgatagag aactgaggaa gaccggaaga
    42661 gatatcgact tccacgtcga ccagatgcgc gtcgacgccc acaatcgctg cacttaacac
    42721 gttggccagc acagtccccc cgggtatccg tcgttcggat cgaaaagtgc acaacaagca
    42781 atcagccgca aatcggcttc agtggccgaa ttataggcag tcgatcccgc tgaattcaat
    42841 tgccgcctcc ccgtctcttg ctgtccctcc tccctgctgt agcgtgccct gcggctgttc
    42901 tgtataatgc ggcatgccaa cagcacgtca ccctcggcgc agtacagtcc ctccatccag
    42961 aatgatgggg ccgtcgggaa ccagtcaggg gacggtattc agacgctcac accgaccgca
    43021 ccttctcgtg accgcgctgt cactggcgct tcttttccct gccatcgctc tcagccaacc
    43081 ctggaacgac aaaccgggct caacgatgca gccggtcgcc cagccgtcac gaacgtcgtc
    43141 ggtgtatatc gggtccgtca tggccctgct cgccacgttt gaagacgccg gcattctccc
    43201 accggaaggt acgccccagg ccaatggcat catcaaggcc gtcattcagt ttcaatcggc
    43261 ttttttaaag agtcatcatc ccgcgatccg gcaattcttt accgacgccc atcgcgtcaa
    43321 gcttggccct cgcgccgagg aagtcgaaac ctcctttcgt cgaagcggct ggtcggcgga
    43381 tacttttgac gcagtgatgg cggtcggtca aacggacaat gcctggaaca ccgagggact
    43441 gagtgaggga ttccgtgaat tcaatatcgg caagccggac ttcgatattc ttgcgcgatt
    43501 gtatgaacag agccgcgccg cattctccac acagggcaag acctttcagg aagtctatgc
    43561 ccagcggcgc cgggaaatgc ccggcgcaaa gtcggaatga ccgtccccta gcagcgagcg
    43621 ccgacgatag cgatcgcatt ttttctcacg atttgtagcc cgaggaggca tcatggcaac
    43681 gaaagcgtac attctcatca aggtcaaggc aggcaaaggc aagtcggtgc tcagcgccct
    43741 gaagagcatt gccggcgtgg agcagattca cgcctgtttc ggccaacccg acattttcgt
    43801 ctttatcaat gtggcggatg aacgggcgct ctcggatgtc gtgattacgc ggattcatgc
    43861 gatcgaggga gtggaggaaa ccgacactca catcgtggcg gaaacctagc tacaggtatt
    43921 cgaccggcac aacccgtcca ccctgcctgg ctgaagcata gcaggcttcg gcaatttcaa
    43981 ccgcacgctt gccgtcttcg atcgtcaccg gaggcggcaa gccgttgcgc acagatttca
    44041 caaaagcgcg gagcgtcccg agcacagtcg gctccgcaat gctcggccag ttgccggcgc
    44101 catgcctggc agagacctgc gccactcgct gcccgcacca gtccgccgat aactgccctt
    44161 cgcttccaat caactccact cgcccgaccc gtcctgacga cacccgcgac acgtccacaa
    44221 ggcacaccag accgccacta gtggtcagac gtcccacgat ccgacgctct gcgccatccg
    44281 gcggcaccac atccatctcg caggacaccg ttagaatctc gtctccggtc aagacacgag
    44341 ccaggtccaa gagatgaata ccggtctcga gcaaacagcc gcgtccgccg aatccccgcc
    44401 cgtcctcact catcccatga ggctccattc gactggccag gctaagatat tggagggcgc
    44461 cgatctgtgc ctgtcgctcc cgcagcacac ccacggcggc atcaaaccgt aacgtgtgtg
    44521 cagtcatcgt gatctgcccg cttctctgcg cggcctgcgc aatggcacgg gcgtccgttg
    44581 ccgatatcgc caacggcttc tcaaccaaca atgctttctg cgcctgtacc acagccaggc
    44641 agatctcccg attgagtgcc ggcggagtca cgacgacgac ggccgccaca cgaggatccg
    44701 cgatcaactc atgatagtcg ggataacagg gcaccgccgg cgcggacgcc agaccatttc
    44761 ccccggcggt ccgacggcgg ctgaccgccg ccagaagcgc ttcaggcagg tcctgcagaa
    44821 ggtgcttggc gtagcggcta ccatgatggc cgacgccgat cagtccgatt ccaagaggta
    44881 aacccggttc gtgagacatc gttgtcgcgc actctaccga ccgcccggca acgggtcaat
    44941 atgccgcgac cactcagccc tcgcctcgat tgacaacgcc caaatacgct ccctatagta
    45001 ccggaggaat tcgcccgaag ccgacgtcaa tcgagaaagg acctcatcgc ccatgactac
    45061 tcaggcagca cccttttcac tggatcagat cggaaccggt acggctcgct tcccgagtgg
    45121 tatggcaatt cccaccgcga cggatgccat ggtggatccc tatatccgca cgcgcgtgag
    45181 caaagacgtg caggtggaat gtatccagtt ctggccgcaa gacaagaccg cctacccagg
    45241 cattgtgctg ttgcatgact ggtggggcat gaattctcag attacggatc ttggcgccag
    45301 attggcctgc gaaggctacg gagtgattat tcccaagtta tacggacgac tgggcggcat
    45361 ggtcaccgcc aacgccgagg tggcagaggc actcatggcc aagtgcaatg aaaagctcct
    45421 gctccaggac atcaatacct gctgcgaata tctcaacacc acggaacaca ccaagcgcaa
    45481 tatccacggt gtcgtcgggt tcgggatggg tgcgtctctg gcgatccggt tcgcctgcca
    45541 gcgcaagcga ctgcgggccg cagtcgcata ctatggaaag gtcaccgctc ccgaccaact
    45601 gacgaatatg atgagcccct tgctctacca ccaagccgag ctggatacct gggcgacgca
    45661 gcaggatgtc gatcatctcc gggccgccgc gagccaaggg aagcgcatcg agatcaagac
    45721 ctacccgggg gcctctcacg ccttctgtga cgagacacgt ccagccggct accaaccaga
    45781 ggccgcaacc caggcctggg ataccaccgt tgcatttctc aaaacctctt ttcaaggaac
    45841 atagcggaca ccgatgggca aggcagccgg tcatggaggg ccgtggagtg cctctcgccc
    45901 gctgctccgc acgcaaagaa agtattcgca ctcatgccga atcattcagt tcgcaccctg
    45961 ctcatcgcct gttgtgtcac cgccttgctg acggcggcag tcgttgccgg ggccgacgag
    46021 ccgtcgcccc aagccaaacc gactcttgcc aatcgcctgc ccgcctttca cgacctggtg
    46081 cgggtcgatg ccgcaccgct ggcggccctc ccaatccagg gcgattcgct gagcctgttt
    46141 aagacagtcg tcggcccgca gctggggttg aacgacgtcg ccatgaccct cggagccaag
    46201 ggtctcacgc ctgccatgag ccaggaactc ctgatccccg atctcaccaa gtcggccaat
    46261 gaactcgtgc ggggacttgc cgcatggcaa ctcacacaag ccgccaaaga ttccagtacc
    46321 tccgccggaa cagaacaacg ggagagcctt cagcgacaga tcgaatccca gatgacctgg
    46381 ttgaccgaag gcacgaccct ggagacacct ctgcgccagc tgttggcgct gctcaaagaa
    46441 ttcccgacac aggaagcagc cgccccccta cagggaacgg ccgctcaaat tgaagcatgg
    46501 gcaatcgata ccgtacatcg ggaatggttc cgtctctaca attggaagga tcaggtccgt
    46561 cagcagcgtg gcctcacacg attgtgcggc acatggcaat ggtcgattca caaccatcag
    46621 aaccatcgcg aggaaaaaac ggccgtgatc ttcgccccgc cgggaatcgc gtcgtcgacc
    46681 agtcctgcgg aaatcatcgt ccttggcgac agcgtctatt tgcgatggga aaccagggcg
    46741 ggtgtgcagg aagacagtct gttattttcc ggagaaggcc aacgcctgga aggaacattt
    46801 gtgaatacgg caggcggatg ggggtcgatt accggcaaac gtaccgcgac ctgcgtgacg
    46861 gacggagggg gaaaacctgc gccgtcgcca cgtcgccagc atcactgaac acgttccgcg
    46921 cgccaggcgg tccaggccac acaggcccat ccggaaataa aggagacacc gccgaaggga
    46981 gtcatcgccc ccatccactt gattcccgcc agcgccacga tatacaaact gccactgaac
    47041 aagagaatcc cgatgcaaaa gagccaaccg gccttcaccg ccagcgaatc ttccgtctca
    47101 cgaaccagcc aggctacgac aaacagaccg agagcatggt acatttggta gcgcgccgcc
    47161 gtttcataga cggcaagcat ggagggatcc agaatcgatt tgagcgcatg ggcgccgaag
    47221 gcacctgcgg ccacggatgt tgcagcgaac aggccgccga gaaacacaaa ccggaacgag
    47281 cgcgccgaca cagccatagg atcctcctga acagccacgg cccttggcaa aggcacggcg
    47341 cgcgattcta tcacgactct cgctaaaaaa tgggcacacc taccccgctt gaatgtcccc
    47401 gttgcctgat ggcgaatcct gacgccgctc aatattgcga atgcggctac gactttacgc
    47461 cgcacttcca gggattcccc ataaaacccg cctccgacat aacggtccgc aaacgcctgt
    47521 tcccggacga aatggggcgc ggcagttttc tgctcggcat tctgctgatc tggctggcgc
    47581 tctctgtcgg cgaactctat cgcatacgca tcgaactacc acgccgcccg aagatggaat
    47641 taggactcag cagtctgatg gtcggcatga ccgatcttgc cgccgcgctt gtcgtcgggg
    47701 cgatcggctg ggcctgcgtg tggattgtgc tggcagctca gagcaagcgt gacggc
//
LOCUS       DAORRQ010000032        47454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_47_length_47454_cov_191.486403, whole genome shotgun sequence.
ACCESSION   DAORRQ010000032 DAORRQ010000000
VERSION     DAORRQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 47454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47454
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47_length_47454_cov_191.486403"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1448
                     /locus_tag="PK224_11420"
     CDS             210..1448
                     /locus_tag="PK224_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB16390.1"
                     /translation="MTDTAGVFRRNSEGQHRRAGDWRSLAGRCLVIGLVLISAAACDR
                     DAPVAPPAGKPVNRAANGMVQLTDPEIARAGIEVMTVKKEPFTFHREFPATVQANENE
                     LAEVTTLIRGRVVEVLVDVGKDVKKGERLALLDSTDLGMAEGAYLKSAARQHEAQLAF
                     ERAENLHEHRAISLAELQRREAEMKTARADAREASHRLALLGLPAQEIKRLEREQTIR
                     SDVAIRAPFAGRVIMRNITRGEVVETSRNCFTIADLSDVWVVANVPEKDVRFIRPKDR
                     VHVMVPAYPHGLFSGTVTYISDVLDPSTRTMRVRVTVLNPDRTLKPEMFAMVRVFASP
                     QPEALAVPLTAVQQDGTGKLLFVRQADNRFESRRVLLGDELDGKTIVLEGLHEGEDVV
                     VKGSFAIKSEFDIHKIEPTP"
     gene            1445..4573
                     /locus_tag="PK224_11425"
     CDS             1445..4573
                     /locus_tag="PK224_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRB16391.1"
                     /translation="MIASLLEFSLRQSILVIGLACMFSVAGLFAFQSIPIDAYPDVTN
                     VQVQVLTEAPGLSPVEVERFITYPIELQMNGLPGLTEIRSLSKFALSQLTVVFEDDVD
                     VYFARQLVLERIMAVKERLPAGIDPVLAPVTTGLGEIYQYYLEGPQAHATDTALVEAE
                     LTNQRTVQDWVLRPLLKSVPGVIDVNGLGGFVKQFQVLVDPDKLRKYSLTLHDIYEAV
                     QKNNANSGGNVLERHAERAIVRGLGLIKTLPDIERIVVKEAGGTPVFVRDVAEVRIGH
                     AVRHGAAVLNGEREVVAGTVLMLRGGNAREVVQAVKRRVDTIQQDGLLSEGLKIIPFY
                     DRIELVTAAINTVRDALIEGIVLVTIVFFLFLGHVRSAIVVTVSLLVTPLMTFLVMQR
                     VGLSANLMTLGGLAIGIGEIADGSLVVVENVYRHLSENHLHQRSRLEVIFRATSEVGR
                     PIVFGILIISVVFLPLMTLHGMEGKMFAPLAYTLVISLLMSVVVTLTLSPVLASLILR
                     GDHPEETRLTRWMKARYQPVLRWTLDHRVPVLLGSTAVVLASLTLLPFVGREFIPILE
                     EGALTPQIVRLPSVSLPESIAIEKQTQKVMLEFPEVQMSVSKIGRPDIAVGPEEPNES
                     DPIVTLRPRNGWTTAQTQSGLVDAIRKRLAEIPGISVLMSQPIQERVDELISGIRTEC
                     AIKLFGDDLDLLYQNAEIIADLMRTVEGVKDVKVEQVAGQPYLTIDIDREKIARYGIN
                     VSDVHDIVTTAVGGKPATQVYEGERRFQLTLRFPEPYRNSIGAIGDIRVRSASGAPIP
                     LSELATIEMREGPARISREQAKRRIYIGFNVVGRDIGSVVDEGRKLLAERIRLPQGYT
                     VAWGGAFENMERANARLLLVVPITLGLVFFLLFWAFHSLRYAALIILNLPFALIGGVV
                     SLWLSDQYLSVPASIGFIELFGLAVGNGIVLVSYINQLRNEGQQTEAAIMTGCVLRLR
                     PVVMTMMTTLLGLLPLVLAQGIGAEVQRPLATVVVGGLFTSTALTLLVLPALYRTFAE
                     QEAAKEHAPEWV"
     gene            4591..7116
                     /gene="mgtA"
                     /locus_tag="PK224_11430"
     CDS             4591..7116
                     /gene="mgtA"
                     /locus_tag="PK224_11430"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HRB16392.1"
                     /translation="MENRRPFWACSPDEVLRDLRAVPEGLSTVEAEQRQVVSASARLT
                     PQRDSQPFRLLLAQFRSPIILILLFASGVSFFLAEHSDALIILAIILVSALLSFWQEY
                     SAARAVAGLLALVQITAQTWRDGRLREVPADDIVPGDIVELSAGSSLPGDARLLDAKD
                     LFVDEATLTGETYPAEKSTGTLTDAVPLQKRTNSLFLGTHVVSGQARAVIVAVGKDTE
                     FGRIAHRMQVRAPETEFERGVRRFGYLLLEVTLLLVFSIFAVNVYLERPVLESFLFSM
                     ALAVGLTPQLLPAIISVNLSHGAKRMAQQKVVVKRLTSIENFGSMNVLCSDKTGTLTM
                     GSMRLHAALDLQGRPSEKVLFLAQLNAMFETGFSNPLDEALRRHRSVDLTGYRKLEEE
                     PYDFVRKRLSILVATPQTHVLITKGAVDSMLTACLDAEQSGGTVVSIDEVRDSIRQQV
                     RELSAQGFRTLGLACRDMGAADRVSKEHETAMTFLGLVAFADPPKPGMAETIATLKGL
                     GVSLKMVTGDQALVAAYVGRAVGLENPHLLTGTDLRGMSDDALRTRANSIDIFAEIEP
                     NQKERIIRALRASGNVVGYLGDGINDAPALHAADVGISVDSAVDVAKEAADLVLLEHD
                     LGVLVQGVREGRMTFANTLKYVFMATSANFGNMFSMAGASLFLPFLPLLPKQILLTNV
                     LTDLPEMTIATDRVDHELIERPRRWDIPFIRRFMLTFGLVSSLFDYLTFGVLLLLLHS
                     TTGQFRTGWFVESVLSASLIVLVIRTRRPCYTSRPSSALLITTLLTGLGTMLLPVTPV
                     GAFLGFEPLPPIFWAAMLGILLAYVVVAEFAKQLFYRQVQNGG"
     gene            7272..8786
                     /locus_tag="PK224_11435"
     CDS             7272..8786
                     /locus_tag="PK224_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16393.1"
                     /translation="MAVSTPRSNTPSYDPQVLLDSQPVTVNVIDPADHSVQFQNRTSL
                     EIFSDMAGSRCHVKIAGKAAPCEFCRMPEALARGGVVSEEVDMPDGRRLLIHWAKAPT
                     TDGRVHVIETIVDVTKRKQDEQSLRQSQKMEALGRLAAGIAHDFNNLLMVVIGHAQRV
                     AQQLGSHPCHHEVEMIGQAGSRAAALTKKLLTFSRRQVLEQRDVPLNTLIRDMEDILR
                     RLIGEQIQTVIVLDPRAGHVLGDPVQIEQVLLNLALNARDSMAEGGILTIETGNADLD
                     EAYVGAHPGAVPGPYVKLVVEDTGCGIDAETLAHIFEPFFSTKEFGKGTGLGLATVYG
                     IVKESRGYIDVTSQPGRGSRFTVMWPRVLTQTGEAEPVAASRAVPAACATILLVEDDE
                     GIRRLVSAVLRDQGYEVLAATDGVEALQMLQLRKGGCDLLITDVILPRMKAAVLAQGA
                     RTMFPNIKVLYISGYSGDMLVSHGVDAQAAYLQKPFLPQAIIEKVAELLPARPQ"
     gene            complement(8953..9321)
                     /locus_tag="PK224_11440"
     CDS             complement(8953..9321)
                     /locus_tag="PK224_11440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB16394.1"
                     /translation="MSKRKNTRVEMNRPIELQGPRGTSQGVVRDFSPGGCRIEQPDAK
                     VHCGMRLTLRISLPDRREPIEIKPAVVTWTGKESFGVEFLSLSQDIRARVKIVYDLLL
                     DAQSSAESERVISLPSVSWT"
     gene            complement(9467..10456)
                     /locus_tag="PK224_11445"
     CDS             complement(9467..10456)
                     /locus_tag="PK224_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16395.1"
                     /translation="MPSFTTSFLVSLLGPPLLIFGTAMATEPPQPAPIALVEQPWAVS
                     LDMTGYRVQIDGVKPDGRRYVFATNSAAAMHLSVTLEAVSGQATEQGCLAHLERIART
                     SPEQPQQDITRYEIRQVPVLEYLLPETKGTAVDQLHLFACVRKDNVYADIHVAKTGFT
                     TGDDSRLRAVLQSLDIVPAPLAGSLDHFRAGSAPYLQGQFRQAIPHYEQAITLERAHS
                     TLGKAVWRLLVHNLGVAYGMTGDLARAKTTLDYGLSRDPANSLFHYHLARTYAGLNDR
                     EKAMQSLHAAFRNDRQREAGDRIPDPRQDVSFRRFMLDPTFRDLAESLMQPAI"
     gene            10842..11441
                     /locus_tag="PK224_11450"
     CDS             10842..11441
                     /locus_tag="PK224_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16396.1"
                     /translation="MQQRTTGLLGFRRRWRVACLLAASLMLAGPSAHATVQVLPEMTL
                     KREGRTITGAKAIRADQTMQDILTAFDRAEHALERQDLSALMQFYASGYNYHGLHVAD
                     VERIWGEVFEHYRALSSTHLFSEMKVVQTEEGTRVELTCTGGLYGTEAQSGARITLDS
                     WFQEVHYLVRDKNGWRFLGNRGDAPRSAPFSSSPHHPLF"
     gene            complement(11452..11904)
                     /locus_tag="PK224_11455"
     CDS             complement(11452..11904)
                     /locus_tag="PK224_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16397.1"
                     /translation="MTRSSWSAPNPIPDSLRRSLKDLRHGLLGLHKSLIVSEQVTYER
                     IYGRVSSAGQLLQLVMNDPWFAWLHPLSHLVVRIDVVLEDQDDTTLEAAQDLLSEART
                     MLRPSEEGDGFERSYYEALQRTPDVVLAHASVKKLLGTAATAASAAAA"
     gene            12209..14923
                     /locus_tag="PK224_11460"
     CDS             12209..14923
                     /locus_tag="PK224_11460"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRB16398.1"
                     /translation="MVEQGQNIQARWYAQSPDELARALDVSLAEGLREDDAARRLSMH
                     GRNELPEAPPPSPWTILGAQFTSLIVWVLIGAAIVSGLLGEWVDTGAILAIVLLNGFL
                     GFVQEYRAEQSLAALKTLAVTYARVIRGGSRRQLSSTELVPGDIIDVEAGDHIPADAR
                     LIHAVALRTQEAALTGESTPVDKSGGVLPDNDLPLADRRNMLFLGTTVTGGKGRALIV
                     ATGRGTELGRIATLMTSVPVEPTPLQRRLEQFGHVLLLLSLGIVVVVFVLGLWRGEPV
                     FDMFLTAVSLAVAAIPEGLPAIVTTTLALGVMRMVTRHALIRRLPAVETLGAATVICT
                     DKTGTLTKNEMTVTRLALDGLVYEVTGEGYGPEGDIIGGDPREGGLQQLLLSALLCND
                     ATLKEVDGSWKVVGDPTEGALLVAGGKAGWRKEDQERAHPVVGEIPFDSERKMMTVVR
                     RSGADIVAYVKGAPDILLGRCRDYLSVEGEVRPLTDSMRESILSINRQFAGQALRVVA
                     LARRRLDQEPSIYDSETIERQLVFLGLAAMKDPLRPEAKVAVELCRSAGIATVMITGD
                     HKETALAIAREAGFPGGATQALSGLELNGLTDGELSARVRDIAVYARVSAEHKLRIVR
                     AWRAQGAVVAMTGDGVNDAPAVREADIGIAMGLTGTDVTKDASDMVVTDDNFASIAAA
                     VEEGRSIYDNIRKAVHYLLSCNLSEVLVMLGSTLLGWPLPLLPIHILWINLVTDGFPA
                     LALAVDPKDPDVMKRPPRDPQARLLDRERFLAVCLQGTVMAMVTLAVFGISLTIWHDE
                     VPFARTMTFTTLVLVQLLHAFSCRHERYSLFQIGVMTNRALVGAVLLSALLQAWILLS
                     AWGQEIFKVVPLRADEWWLMLGLGVLPFVVMELWKVWRRMREPVAEAR"
     gene            complement(14920..15795)
                     /locus_tag="PK224_11465"
     CDS             complement(14920..15795)
                     /locus_tag="PK224_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRB16399.1"
                     /translation="MCTRVLAILTLWMALLFGIEPVSAQTPLPKPDHIVIVIEENHSF
                     AQIIDSPLAPYLNSLARKGALLTNSYGITHPSQPNYIALFAGSIEGVNGNTCPLALTA
                     PNLHSTLTQAGQTFAGYAEDLPAVGATDCVAGSYARKHNPWVNWQSSPINTVPSGDNR
                     PFTDFPTDFHTLPTVSMVVPNQLNDMHNGKDPERIERGDHWLQTHLDAYVQWADTHNS
                     LLIVTWDEDNGKSDNHIPTILVGPMVRQGRFGEQVDHYGLLRTIEDMYGAKPVGFSRQ
                     ASPLTTIWAVPAPTP"
     gene            complement(15812..17542)
                     /locus_tag="PK224_11470"
     CDS             complement(15812..17542)
                     /locus_tag="PK224_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB16400.1"
                     /translation="MFSFNRFYPFLKPYVPRMMAAAVMVMAVAAINLALLRLGGTLWD
                     VITVQHDAARMTEMILLLLGLVLIQGLCSMGHSYLTAWVSQRVMADFRIHLFAHLETL
                     SVNFFSKRRTGELMSRLMNDVTVIQNVVTDTPIDAAKQIVTFIGGAGFLFAMNWQLCL
                     LILVLLPMLVVVAKLFGRRLRALSTKIQDQTASVSTLVEEVIAGIRVVKSFVQTKREE
                     ERFVAQVQSAMELSLRRATIMAWFVPTIIFVTFAAAALVLWYGGRQVIDGTVSPGDLF
                     AFVLFAGILIGPFGSAARVFAQIKEAQGAMQRVFEILDTHAEIADAPGAVDLPPIRGR
                     VRAEHISFAYDPRQPILSDLSFEAKPGELIAIVGPTGSGKTTIMNLLHRFYDPTAGRL
                     TVDGHDVKDVRLDSLYRQIALVPQDTILFGGPIRDNIRYGREDASEEEMIAASKAAHA
                     HEFIVGFPDGYQTIVGEKGINLSGGQRQRVAIARAILKNPRILLLDEATSALDTESER
                     LVQEALERLMVNRTTFVVAHRLSTIQRADRILVLNKGKIVEEGTHAALLEQRGLYHYL
                     YTLRLSELPV"
     gene            complement(17646..17864)
                     /locus_tag="PK224_11475"
     CDS             complement(17646..17864)
                     /locus_tag="PK224_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16401.1"
                     /translation="MPEGKKVRIRVRTVNCVYVGDFLIPPMRNRVSDAINEEQRLFIS
                     LTDVLINDKDRSDFVAINKNLIESIAQL"
     gene            18233..18682
                     /locus_tag="PK224_11480"
     CDS             18233..18682
                     /locus_tag="PK224_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16402.1"
                     /translation="MTVGCAGKTLQYKDDHDRILRIDQAVELLRKSYVERDRSALEAL
                     LLPSGNLDQVQREIHADFDTFRDIQLEFSIERILIEGDNIDVFVHWQGQWKRDPAEVG
                     TRQRGHGRLAWVGTQSILLREAQGDLPFGMGARASVGEDPARGQAKP"
     gene            18685..20361
                     /gene="nadB"
                     /locus_tag="PK224_11485"
     CDS             18685..20361
                     /gene="nadB"
                     /locus_tag="PK224_11485"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRB16403.1"
                     /translation="MSARLSSSKIETDFLVVGSGVAGLRAAIELSRAGRVLMLTKGHP
                     LESNSMYAQGGVAVALSEEDDVGSHLTDTLKAGHGLCRREAVRVLVEEGPERIQELIA
                     WGAKFDKIGKRFAFTREAAHSRSRILRARGDATGNEMVRALMSYAARQPRIQRLDRRF
                     TVDLAVVDGRCCGAIVLNEMTGERTVLPASAVVLSTGGAGQVYARTTNPGNATGDGMA
                     MALRAGALLMDMEFVQFHPTSLYLPSSPPFLLSEAIRGEGGQLRNIKGELFMHRYHPD
                     GALAPRDVVSRAIWSEMAATRARHVYLDVTHLGAAFVKRRFPTIYATCLRYDIDMTEE
                     WIPVSPSAHYMMGGVWTDVQGATTLPGLCAAGEVACSGVHGANRLASNSLLEGLVFGM
                     RAAQSAVAHAGRFPGPVRMPHLEELEQGRPTDFDDPEKLRSSLRRLMWGKVGLVRSGD
                     SLISASAQLVRWMRLLTKPFASRAALEVKNMVQVARCITDAALWRENSVGAHYRSDFP
                     EPNRPGWRQHSQLLFTDGQTSGSAAKERALVLSPPAPVRGRSGKKKAGRS"
     gene            complement(20252..20968)
                     /locus_tag="PK224_11490"
     CDS             complement(20252..20968)
                     /locus_tag="PK224_11490"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRB16404.1"
                     /translation="MGFGSYTGSTIQMAHFSRTAALFSVAAALLLNALPSQADVYQYI
                     DANGTISLTNVPNDPRYRRVIAELPRSRTVISDGELEPVIARHSRAHRLHPALIRAVI
                     KTESDFDPQAVSRAGAIGLMQLMPQTAVRLDVRDSYNPDENIGGGTKYLRQLLDRFNG
                     NLPLALAAYNAGEHTVERYQGLPPIPETRQYVQKVLRYYRTFLTNDRLSSSRTYRAQA
                     LGAKARALAPSLPTRSSDRP"
     gene            complement(21084..21965)
                     /locus_tag="PK224_11495"
     CDS             complement(21084..21965)
                     /locus_tag="PK224_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRB16405.1"
                     /translation="MWVFLNDRFVRKEEALVSVFDHGFLYGDGVYETLRSYGTRIFMR
                     DQHLARLRRSAEAIGLDIPIPESHWPELLHEAMRRNGVGNEQTDAYLRITVSRGAGEI
                     GLDPGLCPRATVVIMAKPLHPSPPVLSRDGVSLTVANTRRNLPEALSPQIKSTNFLNN
                     ILAKRESIAAGTFDSLFLNWKDELTECTVSNLFFISGKTLHTPALDCGILDGITRTIV
                     MTLAQEEGVPVREGHYHLADLQRAEECFLTNTSMEIMPVNRVDSFAIGDGRPGSLTRR
                     LQTRFADSRTRFLEPTS"
     gene            complement(21965..23449)
                     /locus_tag="PK224_11500"
     CDS             complement(21965..23449)
                     /locus_tag="PK224_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRB16406.1"
                     /translation="MSFSSHQAFLSGPPQPLVVVRPQPDVDAFELYCRLAPAERPSFL
                     LESANGTDTTARYSFFGRDPYLTLTSRAQEYQIETGGQIRRYQGSGIQALQQALAEST
                     ITKPEGLPPFYGGAVGYLSYDLVRSFELLPTLAADDLHLPELHMAFFDVVAAIDHHTR
                     QLYLMYCPPLSRFQAEPREKLYREGCDRLAELEARLTSPVTPLTSSPWPTQTTFVPSQ
                     SREAYKTRVRRCQDYIAAGDIYQANLSHRFTLDLHAAASPTGFEGDSTELYRRLRRIN
                     PAPFAGLVRFADLSLVSSSPERLVRLTGSQASTRPIAGTRPRGTGLQQDQLLRAELLA
                     NPKERAEHVMLVDLERNDLGKVCRYGSVRVDEFMTIEQYSHVSHLVSDVVGTLQPGVS
                     PLDLVKAVFPGGTITGVPKLRCMEIIEELEPVRRGLYTGALGYFSWNGDLDLNILIRT
                     LVLTKNRGYLQVGAGIVADSDPDREYDETLAKAGAFFRILEAGR"
     gene            23625..23843
                     /locus_tag="PK224_11505"
     CDS             23625..23843
                     /locus_tag="PK224_11505"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRB16407.1"
                     /translation="MPPSQDPLYAGLGQAVRIGTELLAALIVGGGLGWVVDTYLLDSN
                     PWGLVVGLGLGAAAGVRSAYRSAQRWQS"
     gene            23885..24634
                     /locus_tag="PK224_11510"
     CDS             23885..24634
                     /locus_tag="PK224_11510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRB16408.1"
                     /translation="MEESPLHAFELHDLIPLVPAGVDISINKAVILMWVIVGLVAFLM
                     ISAAAARKLVPGKLQNMAELIVEFIRGIILDTMGEPGMKYFPLIATLFLFILFANLIG
                     LIPGSYTVTSQIIVTAVFAVGVYGLSIVLGFSLHGMKFLGILVPPGTPGWLLPLMIPI
                     ELISQLARPISLAVRLFANMTAGHVILGVLFGLAISGGLLIGWLPFAFTIAMNGLEVG
                     IAFIQAYIFTVLSCVYLGDAVTLHGHSEHAH"
     gene            24671..24901
                     /gene="atpE"
                     /locus_tag="PK224_11515"
     CDS             24671..24901
                     /gene="atpE"
                     /locus_tag="PK224_11515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRB16409.1"
                     /translation="MDAAAAALVGMGLAAAGFAGAGVGIGYIFGKMIEAVARQPEAEG
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGLRK"
     gene            24976..25488
                     /gene="atpF"
                     /locus_tag="PK224_11520"
     CDS             24976..25488
                     /gene="atpF"
                     /locus_tag="PK224_11520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRB16410.1"
                     /translation="MPQFESHFFSSLIFWEILSFGILFFLLYKYAFPSLLGMLEEREK
                     KIKDSLDQAERHRSEAERRLKEYEAKLATAAKDAEALLAQAKERAQRLMEENEQRMTA
                     DAERIKGDATREIETERRKAIQDIRSQTTDLAMMVAEKVVGRALTEGDHRRLADEALD
                     ALAKSYQSRN"
     gene            complement(25495..26316)
                     /gene="larE"
                     /locus_tag="PK224_11525"
     CDS             complement(25495..26316)
                     /gene="larE"
                     /locus_tag="PK224_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRB16411.1"
                     /translation="MVSTTPLIDKVSQARQILREMGSVIVAFSGGIDSSLVLKLAHDE
                     LGAQAVGITAVSPTLPASELDATQKIAAEIGARHRIVETDQLQIPEFVLNDATRCYHC
                     KTDLYSLLETLRSEYADGYIVDGTNVDDLGDDRPGLKAARERGVRSPLLEAGFSKADI
                     RDVARELHLSNWDKPAAACLSSRIPRGITITRSTLSRVERAEDALAQEGFRQYRVRDH
                     GEIARIELAVEELLGLLQPGRRERLAETLKKLGYRFVTVDLEGYRQGGVSLTPPA"
     gene            complement(26360..27742)
                     /locus_tag="PK224_11530"
     CDS             complement(26360..27742)
                     /locus_tag="PK224_11530"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB16412.1"
                     /translation="MTQQPATLLPRQHRSRRPVGRLLAGLLCMALSLPFALAQAADIV
                     PTRSVTANGMTVLFLEQHFLPTVEIHALVKVGSAQDPPDKAGLANLTASLLDEGTLTR
                     TSRQIAEQIDFVGGSLEAHAAEDFTTASTRVLKKDADLGFALLADMLQHPAFHKQEFE
                     RVRTQILGEIVSDDDDPGNVAMKAFHQLIFHGHPYSWPAHGTEETLNKITVADIQQFH
                     AREYLPNQTILVIVGDLTQDQAAALVQTHFGSWKKGPPSPYQIKKPASIERKMVQLIE
                     KDLTQSTIVLGHTGISRTNPDYYAVTVMNYILGAGGFSSRLMDSIRDKQGLAYGIMSQ
                     FDTRLMPGAFFISLQTRTDVTNQAIAGVLTEIKGMRDAPVTDQELNEAKSFIVGSFPL
                     RVDSSAKLANVLAQVELYNLGLDYFTQYPKAIEKVTKDDVLRVAKQYLDPQHYALVVV
                     GSIAKAKVKQ"
     gene            complement(27755..29146)
                     /locus_tag="PK224_11535"
     CDS             complement(27755..29146)
                     /locus_tag="PK224_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB16413.1"
                     /translation="MPNQSTTSNPAPVRFSILACSLLSFILLASQTSPAHAVEPKEYT
                     LSNDMKVILVEVPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTAKYPKGTF
                     SRLVRKNGGMDNAFTSQDFTAYFENLAADRVTLALELEADRMQGLILDANEFKTEREV
                     VKEERRLRNEDDPQGALVEALFAQAFMSHPYHWPVIGWFSDLDAMNLDDLQRHYDTYY
                     SPNNATLIVVGDIKADTLLPTIAKLFEPIPKGPSPKPLAVTEAPQHGERRFLLKREAQ
                     VPFVMMGYRVPNYSSDDSYALNVLESILSHGKSARLYQSLVYEQKTALAVGADYGLMQ
                     ADPGLFYFYAVVKPGEKVEAVEDAVLKEIQRIQTEPPSELELQRAKNQIEAAHIFEQD
                     SNFRQAMLLGEAETIGAGWRKVSQFVERTRAVTAQDVQRAASHYLAADMRTTGTLIPQ
                     PPQTQAASPAQPH"
     gene            complement(29161..30249)
                     /gene="rlmN"
                     /locus_tag="PK224_11540"
     CDS             complement(29161..30249)
                     /gene="rlmN"
                     /locus_tag="PK224_11540"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRB16414.1"
                     /translation="MLQRTDNRTNLLAVTESGMAAFVASLGWPAYRASQILRWLYQER
                     ARTFAEMSNLSQKDREYLTGSCSIERTSAVQIFSSQDGTKKFVLTLADGNQVECVLIP
                     DEDRLTLCLSTQVGCTLDCGFCLTGTLGLQRNLRAHEILDQVLLAQDHLSEGERLTNL
                     VFMGMGEPLANLDAVADAVTRLTNNTWGLGFSGRRITISTAGLASRIKDVAPLKVNLA
                     ISLNATTDTLRQQLMPAANRLHSLEALLAACRAYPLADRDRLTFEYVLLADVNDRAED
                     AARLVKLLRGLRCKVNLIAFNPFPGNPYRRPSDAAIETFQATLRRGHVDAYLRRSRGR
                     DVLGACGQLGRLDTSEAHVALTQIQTRC"
     gene            30361..32598
                     /locus_tag="PK224_11545"
     CDS             30361..32598
                     /locus_tag="PK224_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRB16415.1"
                     /translation="MTALPWRISLSALAVWSVLSSLFLTATAESPRPLPVAPAPCVSA
                     EDCFRSAVALNERSGSPVQRDQTMMLKIDQLRSVMELYPSTIWAKRAGVVLGVLRIER
                     EPVEAAKLLRVVQPDMPVLDDYLRLWIGESLLKQNEPIQAAELLETIPKIVPDSSLIA
                     KAAYRTGEAWYSANVCFRAVDWLERAVALADKDPAAPLALWHLAECYIRENRLPEART
                     ALKQLWLRYPHSPEAREAKARLDTALGGESWTPTAEDHSIRAQAFLGLAMQAEAVEEL
                     RRFLVMAPGHPRRFDARLKLGVAYVRLKQYDQARETFRSLVADRVQESSEATVWLARV
                     YLRQSQGDKLIALARSVAQGSLGGDQRAMVHLFAGVWLEDQGQFDEAIGMFRQVAKLG
                     DSASQRAEGLWRAGWAQYRTARYRDAAETFRSVVELHVNGFEPQAMYWAARADEREKN
                     TSVADQYTRLCQRHAYSYYCQLAARRVSLPPVMPVAADAERPAGDEASRLPENRRPEI
                     ERHVVYQRGIELKILGFAQDAARELGSLTEQYSRDPEVLLAFSTLLSEVGAYHPALRV
                     AKVHFKEKLERSGLPTAPALWTVAYPTGLLPTITAQGVAAVDPHLAAAIIREESQYDE
                     KAVSMVGAVGLMQLMPVTANAVAQRYGFPAVGREELFDQETNIRLGVRYLGQLLEQYS
                     GNLAHAVAAYNAGPIAVNNWIAVHRGREQDEFVELIPYQETRLYVKRVLRSYGEYRRL
                     HNGTS"
     gene            32693..32947
                     /locus_tag="PK224_11550"
     CDS             32693..32947
                     /locus_tag="PK224_11550"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HRB16416.1"
                     /translation="MTLDHLDALEIRIRDLVKLVQDLKRKNASLEDDLRLARERVAVR
                     DDENRRWEQERIDIRSRIEKVLGEIDLLECLDEPKEVAVD"
     gene            32940..33236
                     /locus_tag="PK224_11555"
     CDS             32940..33236
                     /locus_tag="PK224_11555"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRB16417.1"
                     /translation="MTKTIDVEIYGQRYSINGEADESYVKQLADMVDKQMKQVAAGMR
                     SATPAKLAVLAAFNLAHELMESERKSRQGEADADRRVASLMESIDQQMPSILSR"
     gene            33796..35373
                     /gene="rny"
                     /locus_tag="PK224_11560"
     CDS             33796..35373
                     /gene="rny"
                     /locus_tag="PK224_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRB16418.1"
                     /translation="MVPISLSVVAYIFVGLVGAVLGAGLYEVFRRRSALARRAEAEDQ
                     SAQIVQSAQREAENLVKEAKLEAKDLVFQARIELEKEQKAKLAEVSNSERRVSQREEG
                     LDRKLGLLEKRDQEALKREQDLLKREETLVQKDAACAQALKQHREALERVAGLTAEEA
                     KRQLIQDLDSQARLEAAGLAKRLLEEAKENADREAREIIASSIQRVTRDYVNEATISV
                     VPIANDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIISGFDPLRREIAKVSLE
                     RLMHDGRIHPTRIEEIVEKVKVDIEKLMIEEAEKVIFEVGLSDFHPELVKVLGRLKYR
                     TSYGQNNLYHAREAAYICGIMASELKLDVKLAKRGALLHDIGKAVSHEEEGPHAMLGA
                     EIAKKYGENAKVVNAIAAHHEQVEPICPETVLVAAAEALSAARPGARREALESYVKRL
                     EKLESLATVHKGVQKAYAIQAGREIRVIVKQEDLTDPECFQLSRDLAKKIEQELTYPG
                     QIKVTVIRESRFVDFAK"
     gene            35384..36163
                     /locus_tag="PK224_11565"
     CDS             35384..36163
                     /locus_tag="PK224_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRB16419.1"
                     /translation="MKVLMIGDIMGEPGRRSVARLLPKLIANHSIDVVVGNGENVAGG
                     FGITPDLVDDLFDLGVSVITTGNHAWDKKEILDVFPREPRLLRPANYPTGVPGRGSYV
                     FTTPGGESLGVLHLMGRAFMPTIDCPFQVAKREIERLKTQVSAIVVDMHAEATSEKMA
                     MGHYLDGLVTVVAGTHTHVQTADEQILPKGTAYITDIGMTGPLHSVIGIKKELAIEKF
                     LTGMPRRFEVASGPVVFCAVLMELDATLGKALSIERIRVVD"
     gene            36201..37559
                     /gene="xseA"
                     /locus_tag="PK224_11570"
     CDS             36201..37559
                     /gene="xseA"
                     /locus_tag="PK224_11570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRB16420.1"
                     /translation="MTGQTLPLPLLLSVSDVTRLIRDSLEAQFRDIWIEGEITNLRAP
                     SSGHLYFTLKDEQSQLRGVLFRSGASRLRFTLQEGLVIVARGRISVYEPRGEYQLIVD
                     SLEPKGVGAFQLAFEQLKERLAREGLFDEARKRPLPPFPRTVGVVTSRTGAAVRDIVA
                     VLRRRCPVANILIASVPVQGEGAAEQIAEAITTLSGMPQVEVMIVGRGGGASEDLWAF
                     NEEVVVRAIVQSRVPVVSAVGHEIDVTLADFAADYRAPTPSAAAEAVVPVLDEIVERL
                     GEMSDRLYRVVRTLLEMQRHRFERSVGVMRDMRFRVQAHAQHLDELRDGLTRTLTERL
                     TLLHRGMVVRQHALLAQGPHNRIQTALVVIPQLYKRLEQEARRGLLSRRQAVASYMMA
                     LDALSPLAILSRGYSVIQAIPSGRIVRLASDVAVGDVVQARLAEGRLLCLVSEVLPPS
                     MP"
     gene            37617..37865
                     /gene="xseB"
                     /locus_tag="PK224_11575"
     CDS             37617..37865
                     /gene="xseB"
                     /locus_tag="PK224_11575"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRB16421.1"
                     /translation="MAAVKFEYAMARLETIVAELEKGDLPLDDSLKIFEEGIRLSKTC
                     LKMLEDAERKVEILVQEKDGKKRIQAFSPGEDEPERPQ"
     gene            37933..38688
                     /locus_tag="PK224_11580"
     CDS             37933..38688
                     /locus_tag="PK224_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRB16422.1"
                     /translation="MSQKLRPERERLDRVLVSRGLVASREDAARLILAGLVRVDGVVV
                     DKAAKPTLSDAKVELTGPGSPYVGRGGEKLAGALDQFQVDPKGMMCFDVGCSTGGFTD
                     CLLQRGAARVYAVDVGYGQFEWRLRQDPRVVLMERTNIRYLEPSAIRDPIDLIVIDVS
                     FISLTLVLPCVVPYLAASGFVITLIKPQFEVGKGLVGRGGIVRDDGLREGAADKVVAC
                     AQQLGLELADRMESPIEGRKGNREILAWFRRRA"
     gene            38860..39783
                     /locus_tag="PK224_11585"
     CDS             38860..39783
                     /locus_tag="PK224_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB16423.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLLQEGHDVVVVDNLVTGKRKNVPKA
                     AQFYKLDIENPKLERIFRNERPSIVFHLAAQMNVRRSVEDPMFDAQVNVLGTLNVLEQ
                     ASKHGARKVIFSSSGGAIYGEQLAFPAPETHITQPLSPYGISKLCGEHYLSYYHRLSG
                     IQVVSLRYANVYGPRQDPEGEAGVVAIFIQKMLRGEQAVVNGNGRQTRDFVFVEDVVE
                     SNLMAMGPEVEGVYNVGTGIETSVNDLFKIVVDLTKVEFKEVHGPAKRGEQARSVIDS
                     TKLHRDLGWEPKVDLREGLRRTVEYFRDGLG"
     gene            complement(39793..40113)
                     /locus_tag="PK224_11590"
     CDS             complement(39793..40113)
                     /locus_tag="PK224_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB16424.1"
                     /translation="MSFTITPKDLKSRLDKGDKLVLVDVREPWEYAIAKLEGSILVPL
                     ATLQQSLGKLDRDAEIVAVCHHGMRSADATGFLLQQGFGKVKNLIGGIDAWSTQVDPS
                     VPRY"
     gene            40460..40789
                     /gene="cutA"
                     /locus_tag="PK224_11595"
     CDS             40460..40789
                     /gene="cutA"
                     /locus_tag="PK224_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRB16425.1"
                     /translation="MGANGSEVVVFVTAATAEEAERLGRIIVESRLAACANVLNGIRS
                     IFRWENKINVENECLMLIKTTLERYPELEAVIRRHHSYTIPEIIALPVIAGSAPYLEW
                     VREETRK"
     gene            40907..41926
                     /locus_tag="PK224_11600"
     CDS             40907..41926
                     /locus_tag="PK224_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRB16426.1"
                     /translation="MSQQAAETSDAYTVVVFRGSSSKPLRFSFPRKFVRKLLILAAIL
                     VVADLLVISHYVIRTGEVWQLSAFRAEAMGAREQTAAFSAAIDDLKKRLSTMGEVNQR
                     LRVMLGIDASKPAGDLANGRGGEDGPLPDGKTSVQGNGSATSGLEPRQQVSEARDANQ
                     SEVDFATESIEEVTQQVRESLEALVREAKQQEEALESLTQVAEQRSTQWASTPSIWPV
                     RGWVTSAFGPRVSPFTEKPAWHDGLDIGAQANSPVQAPALGRVVTVAFDSKMGNMVKL
                     DHGYGIETVYGHLAKSLVKEGQRVKRGDVVALVGSTGLSTGPHLHYMVKKNGQALDPT
                     KFILD"
     gene            42124..43806
                     /locus_tag="PK224_11605"
     CDS             42124..43806
                     /locus_tag="PK224_11605"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRB16427.1"
                     /translation="MTDLEIARSVPHKHIFEVAQSLGIHSDDLIPFGRYKAKIAPTLG
                     ERIQNRPLGRYILVTAINPTPLGEGKTTTSIGLSMGLCRLGHRAAVTLRQPSLGPVFG
                     IKGGGTGGGRAQVHPMEDINLHFTGDAHAVSASHNLLSAFVDNHLFHGNTLKVDPKGI
                     RWPRTLGVSDRALRDILLGEETGRRPGKFVITEASEVMAVLALATDHADLRQRLGRIL
                     IGLTEGGQMVRAEEFGCAGSMAVLLKDALLPNLVQTLEGTPAFVHAGPFGNIAHGNCS
                     IVSDRLALRCADYVVTEAGFGSDLGAEKFFNIKCRTSGLRPDVGVVVATLRALKLHGG
                     GGIVKSGAPLPPGVTGPNQAALEQGFANLEQHIANVRAHGVPVVVAVNAFKDDSTDEL
                     NWVCERALEGGASAAAVSTHWSDGGRGAEELARAVVKVAAQGTQFSPLYEVTSPIKKK
                     IETIATTIYGAAGVSFSPQAERDVELATRLGFDRLPICMAKTPLSLSHDPAVKGRPSG
                     FTVPIQELRILAGAGFLTAVCSGIQLMPGLPKKPAGERIDVDPQSGQIVGLQ"
     gene            complement(43814..44674)
                     /gene="hflC"
                     /locus_tag="PK224_11610"
     CDS             complement(43814..44674)
                     /gene="hflC"
                     /locus_tag="PK224_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRB16428.1"
                     /translation="MSKQGFILAFVGIVLGLLVLGASPFYIVDVTQNAIVVQLGKPVR
                     NVTEGGLYLKMPFIEEVTYFDKRLLDYDSNAQDVITQDKKTLLLDNFAKWRITDPLKV
                     YQAFQSQRGALQRLHDIIYSELRVELGRHDLAEIVSSARAQLMAVVTQRANEKASAYG
                     IEIQDVRIKRADLPEQNEKAVFSRMQAERERQAKQYRAEGAEEAQKIKSEAEKDREII
                     LAEAYRESEELRGGGDAKAFRIYADAYRQDPHFFEFTRTMEAYRKTLKDKTTILVSPE
                     SEFFRYLKQR"
     gene            complement(44671..45708)
                     /gene="hflK"
                     /locus_tag="PK224_11615"
     CDS             complement(44671..45708)
                     /gene="hflK"
                     /locus_tag="PK224_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRB16429.1"
                     /translation="MVWDPKDPWSKKGDDLDQAFKQAQGQLRNLLPTGGFRNLLLVAF
                     TVFLIWQSAFIVAPDEEGVVKRFGIPVRVVDPGPHMKIPIIESVLQPKVAKLHRVEIG
                     FRKDRQGRQQMVPQEALMLTGDMNILAIEFIVQYKIKSSREYLFNVADIDETIGKAAE
                     ASMREVIGKSKIDEALTTGKAQIQNDTQELLQHILDDYRTGVQVAAVQLQDVDPPEAV
                     AAAFKDVTNAKEDREKLINQAQGYRNDITPKAKGEAAQLVNQAKGYAQARLNRSQGES
                     NRFLATLKEYNQAKDIISKRIYIETLEDVLPHIDKFVLDGKGADRALPYLPLDRFSKP
                     APSSSTQERTP"
     gene            45850..46155
                     /locus_tag="PK224_11620"
     CDS             45850..46155
                     /locus_tag="PK224_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRB16430.1"
                     /translation="MRNPARAAELFHALADPTRLEILDELKEGECCVCELTDRLQAGQ
                     SRLSFHLKVLKDAGMILDRREGRWMYYSVNADALAELDDLVDSLKQAKRARRATGCC"
     gene            46212..47036
                     /locus_tag="PK224_11625"
     CDS             46212..47036
                     /locus_tag="PK224_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRB16431.1"
                     /translation="MDAQAIRELVKEKYGEAAARAKSGGSSCCGASPALMNVDPITSN
                     LYSDQERQGLPADAVAASLGCGNPTALAELHAGETVLDLGSGGGIDVLLSAKRVGPAG
                     KAYGLDMTEQMLALARENQRAAGVENVEFLKGEIEHIPLPDRSVDVIISNCVVNLSGE
                     KERVLAEAFRVLRPGGRLALSDIVVRGAIPSEIRRNLELWAGCVAGALEETEYRDMLA
                     QAGFVEIGIEPTRIYQADDVKELLVGTDLSSDLLVAQVEGKFMSAFIRAKKPAVTV"
     gene            47074..>47454
                     /locus_tag="PK224_11630"
     CDS             47074..>47454
                     /locus_tag="PK224_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HRB16432.1"
                     /translation="MELALSVDEPQVATKRLSFFERYLTLWVALCMVAGVLIGQAVPG
                     LVSSLRGAEIGQGSHINLPIAVLIWLMIVPMMMKVEFASVRDVGKRPVGLLITLFVNW
                     VVKPFSMAFFAWVFFRYVFSAWISP"
BASE COUNT         9045 a        12755 c        15508 g        10146 t
ORIGIN      
        1 gtcgcagcag tgaatcggcg attgcagcag aagtgctcat gaataatgcg ggctaagacc
       61 gctgtggcat ttcaccccgc tctcagggcc ggtagcgtag caaaacgctg ctaccggcat
      121 cccattcctc tcgaaatccg gactctctca cggcatggtc cctgctgttc tcagaaacag
      181 cagggtcgcg caaggcagat gatcgacgca tgacagacac agcgggggtt ttcaggcgga
      241 attcagaggg acagcatcgg cgagccggtg actggcgttc tcttgcggga cgatgcctgg
      301 tgatcgggct ggtactgata tcggcagcag cctgcgatcg ggatgcgccc gtggcgccgc
      361 cggccggcaa gccggtgaac agagccgcca acgggatggt gcagctgacc gatcctgaaa
      421 ttgccagggc cggcatcgag gtgatgacgg tcaagaaaga gccgtttacc ttccatcgcg
      481 aatttcccgc gaccgtgcaa gccaacgaga atgaactggc agaggtcacg acccttattc
      541 gagggagagt cgtcgaggtg ctggtcgatg tggggaagga tgtgaagaag ggtgagcggc
      601 tggcgttgct cgacagtacc gatctcggga tggctgaagg agcgtacctc aagtcggcgg
      661 cgaggcaaca cgaagcgcag ctggcctttg agcgggcgga aaatctccat gagcatcgtg
      721 cgatcagcct ggccgaactg caacgccggg aagcggaaat gaaaaccgcg cgcgccgatg
      781 cgcgggaggc atcgcatcgg ctggcgctcc ttggcctgcc ggctcaggaa atcaaacggc
      841 ttgaacgcga gcagacgatc cggtccgatg tggcgattcg agcgcccttt gcaggtcgcg
      901 tgatcatgcg taatatcacg cgcggggagg tggtggagac ctcgcggaac tgtttcacca
      961 tcgccgacct gtccgatgtc tgggtggtgg ccaacgtgcc ggaaaaggac gtgcgcttta
     1021 ttcgtccgaa ggaccgggtt cacgtgatgg ttccggccta tccgcacggg ttgttctccg
     1081 gcaccgtgac ctacatcagc gatgtcctcg acccatcgac caggacgatg cgcgtccgtg
     1141 tcaccgtgct caatcccgat cggaccttga aaccggagat gttcgccatg gtccgtgtct
     1201 ttgcgtcgcc tcaacccgag gcgctggcgg tgccgctcac ggctgtgcaa caggacggca
     1261 cgggcaaatt actgttcgtg cggcaagcgg acaatcgatt cgaatcgcgc cgggttctcc
     1321 tgggagacga gctggacggc aagaccatcg tcctggaggg gctgcacgag ggtgaagacg
     1381 tggtggtcaa agggtccttc gcaatcaagt cggagttcga tatccacaaa atcgagccca
     1441 cgccatgatt gcctcactgt tggagttctc gctccgccaa tcgatcctcg tcatcggact
     1501 ggcctgcatg ttttccgtgg ccggcctgtt cgcctttcaa tctatcccca tcgatgccta
     1561 ccccgacgtg accaatgtgc aggtgcaggt gttgacggaa gcgcctggcc tctctcccgt
     1621 tgaagtggaa cgcttcatca cctatccgat cgaactgcag atgaacgggc tgccgggcct
     1681 gacggaaatc cgctcgttgt ccaagttcgc gctctctcag ctgacggtgg tgttcgagga
     1741 tgatgtggat gtgtactttg cccgccagtt ggtgttggag cggatcatgg cggtcaagga
     1801 gcggttgccg gcgggtatcg atccggtcct ggcgccggtc acgactggac tgggagagat
     1861 ctaccaatac tatctcgaag gcccgcaagc ccatgcgacc gatacggcgc tcgtagaggc
     1921 cgagttgacg aatcagcgga cggtgcagga ttgggtactc cgccccttgc tgaagagtgt
     1981 gccgggcgtg atcgatgtga acggcctggg cggattcgtg aagcagttcc aagtgctggt
     2041 ggatcccgac aagttgcgca aatacagcct gacgctgcac gacatctatg aggcggtgca
     2101 gaagaacaat gccaattccg gcggcaatgt gctggaacgg catgcggaac gcgccattgt
     2161 gcggggactg ggactcatca aaaccctgcc cgacatcgag cgtatcgtcg tgaaggaagc
     2221 gggcggcaca cccgtgttcg tccgtgatgt ggcagaggtt cgtatcggtc atgcggtgcg
     2281 ccatggcgcg gccgtgttga acggcgagcg ggaggtggtg gcgggcacgg tgttgatgtt
     2341 gcgcggaggc aatgcacgtg aagtcgtcca agccgtgaaa cgccgggtcg acacgattca
     2401 gcaggacggg ttgttgtcgg aaggcctcaa gatcatcccg ttttatgacc ggatcgagct
     2461 cgtgacagcg gccatcaata ccgtgcgcga tgccttgatc gagggcatcg tcctggtgac
     2521 catcgtattt ttcctatttc tgggccatgt gcggagcgcg atcgtcgtca ctgtgtctct
     2581 gttggtcacg ccactgatga cgttcctggt gatgcaacgc gtggggcttt ccgccaacct
     2641 catgaccctg ggtggcctgg cgatcggtat cggtgaaatt gccgacggat cgttggtcgt
     2701 ggtggagaat gtctatcgcc atctctccga aaatcatctg catcagcggt cccggcttga
     2761 ggtgattttc cgggccacca gcgaggtagg gcggccgatc gtgttcggca tcctgatcat
     2821 cagcgtcgtc ttccttcccc tcatgactct gcacgggatg gaagggaaaa tgtttgcccc
     2881 cctggcctat acgctggtga tttcgctgct gatgtcggtg gtcgtcacct tgaccctctc
     2941 gccggttctg gcgtccctga tcctgcgcgg cgaccatccg gaagagaccc ggctcacccg
     3001 gtggatgaag gcccgctatc agccggtgct ccgttggacg ctcgaccatc gggtgccggt
     3061 gttgctggga tcgacggcgg tggtgctggc gagtctgacc ctgctgccgt tcgtgggacg
     3121 ggaattcatt ccgattctgg aggagggcgc gttgacgccg caaatcgtgc ggctgccgag
     3181 tgtctcgttg ccggagtcga tcgcgattga gaagcaaacg cagaaagtca tgctggagtt
     3241 tcctgaagtg cagatgtcgg tgagcaagat cggacgcccc gatatcgccg tcggtccgga
     3301 ggagccgaat gagagcgatc cgatcgtgac gctgcgtccg cgcaacggtt ggaccacggc
     3361 acagacccaa tcgggattgg tcgatgcgat ccggaagcgt cttgcggaga ttcccggtat
     3421 ctctgtcctg atgagtcagc cgatccagga gcgggtcgat gagttgatct ccggcatccg
     3481 gaccgaatgc gccatcaagt tgttcgggga cgatctcgac ctgctgtatc agaacgccga
     3541 aatcattgcg gacctgatgc ggaccgtgga aggcgtgaaa gacgtcaagg tcgaacaggt
     3601 tgccgggcag ccctatctga cgatcgacat cgatcgggag aaaatcgccc gttacgggat
     3661 caacgtgtcg gatgtgcacg acatcgttac gacggcggtg ggcggcaaac cggccacgca
     3721 ggtctatgag ggggagcgtc gatttcagct gaccctgcgt ttcccggagc cctatcgcaa
     3781 cagtatcggg gccatcggag atatccgggt acgatccgca tccggcgcgc cgattccctt
     3841 gagcgagctc gcgaccatcg agatgcgcga aggcccggcg cgtatcagcc gtgagcaggc
     3901 gaagcggcgg atctacatcg ggttcaatgt ggtggggcgc gacatcggca gtgtcgtgga
     3961 tgaaggccgg aagttattgg ccgagcggat ccggttgccg caggggtata cggtggcgtg
     4021 gggcggggcg ttcgagaata tggagcgcgc gaatgcgcgg ttgctgctgg tggtgccgat
     4081 cacgctcggg ttggtcttct ttctgctctt ctgggcgttt cactcgctgc ggtatgccgc
     4141 cttgatcatt ctgaatctgc cgttcgcctt gatcggcggt gtcgtgtcgc tgtggctcag
     4201 cgatcagtat ctgagtgtgc ccgcctcaat cgggtttatc gaactgttcg ggctggcggt
     4261 gggaaacggc atcgtgctcg tctcctacat caatcagttg cggaatgagg ggcagcagac
     4321 ggaggcggcg atcatgacgg gctgtgtctt acggcttcgc ccggtcgtga tgacgatgat
     4381 gacgacgttg ctcggcctcc tgccgctggt cctggcccag gggatcggcg ccgaagtgca
     4441 gcggccgttg gccaccgtgg ttgtcggcgg cctgttcacc tcgaccgcat tgaccttgct
     4501 ggtcctcccg gccctgtacc ggacctttgc cgaacaggag gccgcgaagg aacatgcccc
     4561 ggagtgggtg tgaagggagt tcacgagacc atggagaata gacgaccctt ttgggcctgt
     4621 tctccggatg aggtgcttcg cgacttgcgt gccgtaccgg aggggttgag caccgtcgag
     4681 gccgaacagc ggcaggtcgt ctccgcgtcc gcccggctca cgccccagcg agatagtcag
     4741 ccgttccggc tcttgctggc ccagttccga agtcccatca tcctcatcct gctcttcgct
     4801 tccggagtgt cgttttttct cgcagaacat agcgatgcgc tcatcatcct ggccatcatt
     4861 ctggtcagcg ccctgttgag tttctggcag gaatacagcg cggcccgcgc cgtggccggc
     4921 ctgttggcgc tcgtgcagat cacggctcaa acatggcggg acggccggtt acgcgaggtg
     4981 cctgccgacg acatcgtgcc gggcgatatc gtggagcttt cggctgggtc gagtttaccc
     5041 ggagacgcgc ggctgctcga tgccaaggat ctgttcgtcg atgaagcgac cttgacgggg
     5101 gagacctatc cggcagagaa atcgacagga acactcacgg acgcggtccc cttgcagaag
     5161 cggacgaaca gcctgtttct gggcacccat gtagtgagcg ggcaggcgcg ggccgtgatc
     5221 gtggcggtgg gcaaggacac ggaattcggc cgcattgccc atcggatgca ggtgcgggcg
     5281 ccggaaacgg aatttgagcg gggcgtgcgc cgattcggat atctgttgct ggaagtcacg
     5341 ctgctgctgg tgttctcgat ttttgccgtc aatgtgtacc tggagcgccc ggtccttgag
     5401 tcctttctgt tttccatggc gttggccgtc ggcctgacgc cgcaactgtt gccggccatc
     5461 atcagcgtca atctctccca cggcgccaag cgaatggcgc agcagaaggt ggtcgtcaaa
     5521 cgcctgacct cgattgaaaa cttcggcagt atgaacgtgt tgtgctccga caaaaccggc
     5581 accctgacca tggggtccat gcgcctgcac gcggcactgg atcttcaggg ccggccgagc
     5641 gagaaggtgt tatttctcgc ccagctgaac gcgatgttcg aaacgggatt ctcgaatccg
     5701 ctggatgaag cgttgcgccg acaccggtcg gtcgatctca ccggttatcg caagctggag
     5761 gaagaaccgt acgacttcgt ccgcaaacgg ctctcgatcc tcgtggcgac cccgcagacg
     5821 cacgtgttga tcacgaaggg ggccgtggac agcatgctga cggcctgtct cgatgctgaa
     5881 cagtcgggcg gcaccgtggt gtcgatcgat gaggtccggg attccattcg acagcaggtt
     5941 cgcgaactga gtgcgcaagg atttcgcacg ctggggctgg cctgccgtga catgggcgct
     6001 gctgatcgtg tctccaagga acatgaaacg gcgatgacct ttctgggtct ggtggccttc
     6061 gccgatcctc cgaagcccgg catggccgag accattgcga cgctcaaagg cctcggggtc
     6121 tcgttgaaaa tggtgaccgg ggatcaggcg ctggtggcgg cgtatgtcgg gcgggcggtt
     6181 gggctggaga atccgcacct gcttactggg actgacctgc gcggcatgag cgacgacgcg
     6241 ctacgcacgc gcgcgaacag catcgatatc ttcgcggaaa tcgagcccaa tcaaaaggaa
     6301 cgcatcattc gtgcgctgcg cgcttccggc aatgtggttg gatatctcgg cgacggcatc
     6361 aacgatgccc cggctctcca cgcggcggat gtcggcattt cggtcgatag cgccgtcgat
     6421 gtcgccaaag aagcggccga tctcgtcctg ttggagcatg atttgggcgt gctggtgcaa
     6481 ggcgttcgcg agggccgcat gaccttcgct aatacgttga aatatgtgtt tatggccacc
     6541 agcgcgaatt tcggcaacat gttcagcatg gccggggcct cgctgttcct gccgtttctc
     6601 ccgctgctgc cgaagcagat cctgctgacg aatgtcctga ccgatcttcc ggaaatgacc
     6661 attgccaccg accgggtcga tcatgaactg atcgagcggc cccgccgatg ggatattccg
     6721 ttcatccggc gattcatgct caccttcggt ctggtgagtt ccctgttcga ctacctcacg
     6781 ttcggagtcc tcttgctgct gttacactca acgaccggcc aatttcgcac cggttggttt
     6841 gtcgagtccg tactgtcggc ctcgttgatc gtgctggtca ttcgcacccg ccggccctgt
     6901 tataccagcc ggccctcttc cgcgctgttg atcacgacgc tcctgacggg actggggacc
     6961 atgttgctgc cggtgacacc ggtgggagcg ttcctgggat tcgaaccgct gccaccgatc
     7021 ttctgggcgg cgatgctcgg aattctgttg gcctatgtgg tggtggcgga gttcgcgaag
     7081 cagttgtttt accggcaggt tcagaatggg ggttagcgtg gcgaggccgt cggaagggag
     7141 gtgagaagca ccgtcacgtt acagctccct gtaatcgatc atctccttga ctgtgcgtgg
     7201 tgatgtagta caaccaaaga aagaagttca gaccctgtgt accccccgct gccgattctc
     7261 gtgaggacgt catggccgtt tccacgccgc gctccaatac tccgtcgtat gatccgcagg
     7321 tgctgctcga ttcacagccg gtgaccgtga atgtgatcga ccctgccgac cattccgtgc
     7381 aattccaaaa tcgaacctcg ctggaaatat tcagtgacat ggccggctcc agatgccatg
     7441 tgaaaatcgc ggggaaggcg gctccctgtg agttttgccg catgcctgag gcgttggcgc
     7501 ggggaggcgt ggtgtccgag gaggtggaca tgcccgatgg ccgccgtctc ctgatccatt
     7561 gggccaaggc cccgacgacg gacggacggg tacacgtgat tgaaacgatc gtggatgtga
     7621 cgaaacgcaa gcaggacgag cagagcctgc ggcagtcgca gaagatggag gcactcgggc
     7681 ggctggcggc cggcatcgcg cacgatttta acaatctgct catggtggtg atcggccatg
     7741 cgcaacgggt ggcacagcag cttggctcgc atccttgcca tcatgaagtg gagatgatcg
     7801 ggcaggcggg gagcagggcg gccgccttga cgaagaagct gctgacgttc agccggcgcc
     7861 aggtgctgga gcaacgggat gtgccgctca atacgttgat tcgcgacatg gaagatattc
     7921 tgcgccgttt aatcggcgag cagattcaga cggtgattgt gctcgatccc agagccgggc
     7981 atgtgttggg cgatcccgtg cagatcgagc aggtgttgct caatctggcc ctcaatgcgc
     8041 gcgattccat ggcggaaggc gggattctca ccatcgagac gggcaatgcc gatctggatg
     8101 aggcatacgt gggtgctcac cccggcgcgg ttcctggtcc ctacgtcaaa ctcgtggtgg
     8161 aggataccgg atgcgggatc gacgccgaga cgctcgccca tatctttgaa ccgttcttca
     8221 gcacgaagga gttcggcaaa ggcaccggct tggggttagc caccgtctat ggcattgtga
     8281 aggagagccg tggctatatc gatgtgacca gtcagccggg acgcggttcc cggtttacgg
     8341 tgatgtggcc tcgggtgctg acgcaaacag gagaagccga gccggtggcg gcgtctcgcg
     8401 cggttccggc cgcttgcgcg accatactgc ttgtggaaga cgatgagggc attcggcgtc
     8461 tcgtctcagc tgtgctccga gaccaggggt atgaggtgct ggcggccacc gacggcgtcg
     8521 aggccctgca aatgctgcag ttgcgaaaag ggggatgcga cctcctgatc acggatgtga
     8581 tcctgccgag aatgaaagcg gccgtgctgg cacagggggc gaggacgatg tttccgaaca
     8641 tcaaggtgct ctatatttcc ggttattccg gcgacatgct ggtctcgcac ggggtcgatg
     8701 cgcaagccgc ctatttgcag aaaccgtttc tcccccaggc cattatcgaa aaggtggccg
     8761 aactcctgcc cgcgcgcccg cagtaggcgg gccgagatac cgattcttcc gtctcattct
     8821 gccaggtgca ggcaacaccc ttcccatcgc gcatcggtga ttttattgcg atctgttcgt
     8881 gtgaccgggg ctgtgccgtg tacgcgccga agtgatgtga catcgacagg gcatgtgggt
     8941 ttcttgtgat gattatgtcc aagagacgga ggggagggag atgactcgtt cgctctctgc
     9001 gcttgactgg gcgtccagga gcagatcgta cacaattttc acacgggccc gtatgtcctg
     9061 agagagcgat aggaattcga ccccgaacga ctccttgccg gtccaggtga caacggcggg
     9121 tttgatttcg atgggttccc ggcgatcggg cagtgagatg cgcagcgtca atcgcattcc
     9181 gcaatgcacc ttggcgtcag gctgttcgat ccggcacccg cccggagaga agtctcgcac
     9241 gacgccctga ctggtcccgc gaggcccctg aagctcaatg ggccggttca tctccactcg
     9301 tgtgttcttc cgtttcgaca tcgatcgccc tttccgcgct gcgcctctgc gtgacaggat
     9361 caagcccgtg ctgttcggaa acaaccctgc aacaggaacg gcgtgtgaga catgcaccga
     9421 ccccgtccga tgcgggcttc agtctgttgg cgctccagac ctccgttcaa atagcgggtt
     9481 gcatgaggga ctccgccaag tccctgaatg tcgggtccag catgaatcgc ctgaatgaaa
     9541 catcctgtcg tggatccggt atgcggtcac ccgcctctcg ctgacgatcg ttgcggaagg
     9601 cggcgtggag ggattgcatg gccttctccc ggtcgttcag ccccgcgtag gtgcgggcga
     9661 ggtggtaatg aaacagtgag ttggcaggat ctcgtgagag accatagtcc aacgtcgtct
     9721 tggcgcgtgc caggtcaccg gtcatgccgt aagccacccc caggttatga acgagcaggc
     9781 gccagacggc cttgccgagg gtcgagtgtg ctcgttccaa cgtgatcgcc tgttcatagt
     9841 gcgggatggc ttgcctgaat tgcccttgaa ggtagggtgc gctgcctgct cgaaagtgat
     9901 cgaggctgcc ggccaggggg gcggggacga tgtcgaggct ctgcagcact gctcgcaatc
     9961 gtgaatcatc tccggtcgtg aaaccggttt tggcaacatg aatgtctgcg tagacgttgt
    10021 ctttacggac gcaggcaaac aggtggagtt gatctaccgc tgttcccttg gtctctggca
    10081 gcagatactc gaggacaggt acctgcctga tttcgtatcg cgtgatgtcc tgctggggtt
    10141 gttccggtga tgtccgcgcg atgcgttcca gatgggcaag acagccttgt tccgtagcct
    10201 gaccggacac cgcttctaac gtgaccgaga gatgcatcgc tgcggcgctg ttggtggcga
    10261 agacatatcg ccgtccatcc ggcttgaccc cgtcgatctg cacacggtaa ccggtcatgt
    10321 cgaggctcac ggcccacggc tgttccacga gggcgatggg tgcgggctgc ggtggctccg
    10381 ttgccatggc ggtcccgaat attaaaaggg gcgggccgag taggctgacc agaaatgatg
    10441 tcgtgaatga tggcatggga tctcctggcc ctctttcgtt cgggtgtcgg tcgtgaggtg
    10501 actgtagtct aacgtttacg ccccgcctgc caaagaaaaa gtgcgaaacc agcgtcacgg
    10561 cgccctgctg ttccggcacg ttcgcggaga gttggtcggc tcagtctgtg gcgtcctgca
    10621 acagtgtgga ggggagtctg gggtatttgc cttcagcggg ggacggcgta gtgtcggctg
    10681 gtcggaggat ctgccccact catgcatgtc gcgcgggcgt gattggcgaa tcgggcctgt
    10741 ccccctcaac ggctgttggg taccggcttt gcattgtgtg aggcaaggat gtatctgtat
    10801 cgtgggccgg tcatgccgac cgcagaacgg aaggagctag aatgcagcag cgaaccacgg
    10861 ggctactcgg gtttcggcga cggtggcgag tcgcctgctt attggcggca agcctcatgc
    10921 tggcggggcc gtcggcgcat gccaccgtgc aggtgttgcc tgagatgacg ctgaagcgtg
    10981 aggggcggac catcaccgga gccaaggcga ttcgcgccga tcagacgatg caggacattc
    11041 tgacggcctt cgatcgtgcg gagcacgcgc tggaacggca ggatctcagc gcgctgatgc
    11101 agttttatgc gtccggctac aactaccacg gcttacatgt ggcggacgtg gagcgcatct
    11161 ggggcgaggt cttcgagcac tatcgcgcgt tatcctcgac gcacctgttt tccgagatga
    11221 aggtggtgca gaccgaagaa gggacccggg tggagttgac gtgcaccggc ggtctttatg
    11281 gtacagaggc gcagtcaggc gcccgcatca cgctggatag ttggttccag gaggttcact
    11341 atctggtgcg ggacaagaac ggatggcggt ttctcgggaa tcgaggggat gcgccgcgtt
    11401 cggcgccctt ctcctcgtcc ccgcaccacc ctctgttctg agcgggagtg attaggctgc
    11461 cgccgcggat gcggctgtgg cagcagtgcc gagcagtttc ttgacgctgg catgggccag
    11521 gaccacgtca ggtgtccgtt gcagcgcttc atagtagctg cgttcaaaac catcgccctc
    11581 ctcagaggga cgcagcatcg tgcgcgcttc cgacaggagg tcctgtgccg cttccagggt
    11641 cgtgtcgtct tgatcttcga ggacgacatc gatgcggacg acgaggtgag acaaggggtg
    11701 aagccaggcg aaccaggggt cgttcatgac cagctgcaac agttgtcccg ccgaggagac
    11761 gcgcccgtag atgcgttcat aggtgacttg ttcggagacg atgagggact tgtgcaggcc
    11821 aagcaggccg tggcggaggt ccttgaggct gcggcgcaaa ctgtccggaa tcggattcgg
    11881 agccgaccag gaagaacgtg tcatatacca ccaatcctaa cagatccaca gcttcaagcg
    11941 acggttgttc ccaccacagg aatgacgccc gatccagggc cgatgctccg tccttcgtgg
    12001 atactctatc ggtgtgtttt cgcaaggatt gagtcttcgg cacggggagg gccacatttt
    12061 tttgtaaggc cgcgtcgtag acgtgatgaa aaccaggggt ttctcctgga agctgaagca
    12121 cttacctgaa tatcggttgc gctcgggatg tgtacgtagg ggatccggat cccaacgcgg
    12181 aaccggtaag gcggagagat tggaggctat ggtcgagcag ggacaaaata tccaagcccg
    12241 gtggtacgca cagtcacccg atgaactcgc gcgggcgctt gatgtgagcc tggccgaggg
    12301 gctgcgcgag gatgacgctg cgcgccgcct gtcgatgcac ggccgtaatg aactgccgga
    12361 agccccgccc ccttcccctt ggacgatcct tggggcgcag ttcaccagcc tgattgtctg
    12421 ggtgctgatc ggcgctgcga tcgtctccgg cctgctcgga gaatgggtcg ataccggcgc
    12481 catcctcgcc attgtcctgc tgaacggatt tctgggtttc gttcaagagt atcgagccga
    12541 acagtcgctg gcggcattaa aaaccctggc ggtgacctac gcgcgggtga ttcgcggcgg
    12601 ctcgcggcgt cagctgtctt ccaccgaatt ggttcccggc gacatcatcg acgtagaggc
    12661 gggggatcac atcccggcgg atgcgcgcct cattcacgcc gtggcgttgc gcacgcagga
    12721 ggccgcgctg accggggaat ccacgccggt cgataagtca ggcggggtgc tgcctgacaa
    12781 cgacctgccc ctggccgatc ggcggaacat gctgtttctg gggaccaccg tcacgggagg
    12841 gaagggccgt gcgctcatcg tcgcaaccgg tcgtggaacc gagctgggac ggatcgccac
    12901 gttgatgacg tcggtgcctg tcgagccgac cccgctccag cggcggttgg agcagttcgg
    12961 tcatgtcctg ctgctgctgt cactcgggat tgtcgtcgtt gtgttcgtgc tgggcttgtg
    13021 gcgaggcgag ccggtcttcg acatgtttct cacggccgtg agtctggcgg ttgcggccat
    13081 cccggaaggt ctgcccgcga ttgtgacgac gactctggcg ctcggcgtca tgcgcatggt
    13141 gacgcgccat gcgttgatcc gtcgattgcc ggccgtggag acgctcgggg cggcgacggt
    13201 gatctgtacg gacaagaccg ggaccctgac gaagaacgaa atgacggtga cgcgcctggc
    13261 cctcgatgga ctggtgtatg aggtgaccgg agaagggtat gggccggagg gcgacatcat
    13321 cggtggcgat ccgcgcgagg gaggcttgca gcagcttctg ttgagcgccc tgttgtgcaa
    13381 cgacgcgacg ctcaaggaag tcgatggcag ttggaaagtg gtgggcgatc ccacggaagg
    13441 ggcgttactc gttgcgggcg gcaaggccgg atggcggaaa gaagaccagg agcgggcgca
    13501 tccggtggtg ggggaaattc ccttcgattc cgaacgcaag atgatgacgg tggttcgacg
    13561 atcgggcgca gacatcgtgg cctatgtgaa gggagctccg gatattctgc tcggccgttg
    13621 tcgcgactat ctgtcggtcg agggagaggt gcggccgctc accgattcga tgagagagtc
    13681 cattctgtcc atcaaccggc agttcgccgg gcaagccctt cgtgtcgtcg ccctggcccg
    13741 gcggcgcctc gatcaagagc cgtccattta cgactccgag accattgagc ggcagttggt
    13801 ctttctcggg ttagccgcca tgaaagatcc cttgcgtccc gaagccaagg tggccgtcga
    13861 gctctgccgg tcggcgggta tcgcgacggt gatgattacc ggtgatcaca aggagacggc
    13921 cttggccatt gcgcgtgagg cggggttccc gggcggcgcg acgcaggcgc tgtccggcct
    13981 ggagttgaat gggctgaccg atggcgagtt gtccgcgcgg gtccgggaca ttgcggtgta
    14041 tgcgcgggta tcggccgagc acaaactgcg catcgtgagg gcctggcgcg cgcaaggggc
    14101 ggtggtggcg atgaccggcg acggggtgaa cgatgccccg gcggtgcggg aagcggatat
    14161 cgggattgcg atgggcctga ccggcacgga tgtcacgaaa gatgcgtcgg acatggtcgt
    14221 caccgacgac aatttcgctt caatcgcggc ggcggtcgag gagggtcgca gtatctacga
    14281 caatattcgc aaggccgtgc attatctctt gtcgtgcaat ttgagcgaag tgctggtgat
    14341 gttggggagt acgttgctgg gctggcccct gccgctgctt ccgattcata ttctgtggat
    14401 caatctggtg accgacggat ttccggctct ggccctcgcc gtcgatccga aagatcccga
    14461 tgtgatgaaa cggccgcctc gcgatccgca agcccgcctg ttggatcgag agcgatttct
    14521 ggctgtctgt ctccagggca cggtgatggc catggtgacg ctggccgtct tcgggatctc
    14581 tctcacgatc tggcacgatg aggtgccctt cgcacgaacc atgaccttta ccacgctggt
    14641 gttggtacag ctgctgcacg ccttcagttg tcgccatgaa cggtattctc tgtttcaaat
    14701 cggggtgatg accaatcggg cgcttgtggg ggccgtgctg ctctctgccc tgttacaggc
    14761 ctggattttg ttgagcgcct gggggcagga gattttcaaa gtcgttccgc ttcgagccga
    14821 cgaatggtgg ctgatgctgg gactcggcgt gctgccgttc gtggtgatgg aactgtggaa
    14881 ggtgtggagg cgaatgcggg aacctgtcgc tgaagcgcgc tagggggtgg gagcaggtac
    14941 ggcccaaatg gtggtgaggg ggctggcttg ccggctgaac ccgaccggct tcgcgccgta
    15001 catgtcttcg atcgtgcgca acaatccgta atgatcgact tgctcgccga accgtccctg
    15061 ccgcaccatc gggcccacca gaatcgtcgg gatatggttg tctgactttc cgttgtcctc
    15121 atcccaggtg acgatcagca ggctgttgtg ggtgtccgcc cattgcacat aggcatccag
    15181 gtgcgtctga agccagtggt ccccgcgctc gatgcgttcg ggatccttgc cgttatgcat
    15241 gtcgttcagt tgattgggta cgaccatgct gacggtgggt agggtatgga aatctgtcgg
    15301 gaagtcggtg aacggacgat tgtcgccgga cggcaccgtg ttgatgggag acgattgcca
    15361 gttgacccat gggttatgct tgcgcgcgta ggatcctgcg acgcaatcgg tggcgccgac
    15421 ggcgggcaga tcttcggcat acccggcgaa ggtctggccg gcttgcgtca gggtgctatg
    15481 aagattcggg gccgtcaacg cgagcgggca ggtgttcccg ttgacccctt cgatggatcc
    15541 ggcaaacaag gcaatatagt tgggttgact gggatgggtg atgccgtaag agttggtcag
    15601 cagcgcgcct ttgcgggcca gggagttgag gtaaggagct aaaggcgagt cgatgatctg
    15661 agcgaacgaa tggttctcct cgatcacgat cacaatatgg tcgggcttgg gcaacggcgt
    15721 ctgggcggaa acgggttcga tcccgaagag gagagccatc cacagtgtga gtatggccag
    15781 gacgcgtgtg cacatgtgaa gcccctccgg gttacacggg cagttcgctg agtcgcaggg
    15841 tatacagata atgatagagg ccccgctgct ccagcagtgc ggcatgggtc ccttcttcca
    15901 cgatttttcc tttattcagg accaggatgc gatcggcgcg ctgaatggtg ctgagccgat
    15961 gcgccacgac gaaggtggtg cggttgacca tcaggcgctc cagcgcttcc tgcaccagcc
    16021 gttcggactc cgtatccagc gcggaggtgg cctcgtcgag caggagaatt ctgggattct
    16081 tcagaatggc tcgcgcaatg gcgacacgtt ggcgttgacc gcctgagaga ttgatgccct
    16141 tttctccgac gatcgtctga tagccgtccg gaaatcccac gatgaactca tgggcatgcg
    16201 cagccttgct ggcggcaatc atctcttctt cgctcgcgtc ttcccgcccg taacgaatgt
    16261 tgtcccggat gggcccgccg aacaggatcg tgtcttgcgg caccagggcg atctggcggt
    16321 acaggctgtc gagccggacg tctttgacat cgtggccgtc caccgtgagc cgtccggcgg
    16381 tcgggtcgta gaatcggtgc aagaggttca tgatcgtcgt cttgcctgac ccggtgggtc
    16441 cgacgatcgc aatcaactcg ccgggcttgg cctcgaagga gagatccgag aggatcggct
    16501 ggcggggatc gtaggcaaag ctgatgtgtt ccgcgcgcac acgaccacgg atgggcggca
    16561 gatcgacggc gcccggggca tcggcgattt cggcatgggt gtcgagaatc tcaaagacgc
    16621 gctgcatggc cccttgggct tccttgatct gcgcgaacac ccgggctgcc gatccgaacg
    16681 gtccgatcag aatgcctgca aatagcacga aggcaaacag gtcgccgggg gacaccgttc
    16741 cgtcgatgac ctggcgcccg ccgtaccaca agacgagggc ggcggcggca aaggtgacga
    16801 agatgatcgt cggcacgaac caggccatga tggttgcacg gcgcagtgac agctccatcg
    16861 cggactggac ctgagccacg aagcgttctt cttcccgttt cgtttggacg aaggatttca
    16921 cgacgcggat ccctgcaatg acttcttcga ccagcgtgct gacggaggcg gtttggtcct
    16981 ggattttggt ggacagcgcc cggagtcgcc gcccgaacag tttggccacg acgaccagca
    17041 tcgggagcag caccagaatg agcaggcaga gttgccagtt catggcgaac aggaagcccg
    17101 cgccgccgat gaaggtgacg atttgtttgg cggcgtcgat gggcgtatcg gtcacgacgt
    17161 tttggatgac ggtgacatcg ttcatgaggc gggacatcaa ttcgccggtt cggcgcttcg
    17221 agaagaagtt gaccgacagc gtctcgagat gtgcgaagag atggatccgg aaatcggcca
    17281 tcacccgttg ggagacccag gcggtgagat aactgtgtcc catcgaacat aacccctgga
    17341 tcaggaccaa gcccaacaag agcaggatca tttctgtcat gcgtgcggca tcgtgctgga
    17401 cggtgatgac gtcccaaagg gtgccgccta agcgaaggag ggcaaggttg atggcggcga
    17461 cagccataac catgacggcc gccgccatca ttcgagggac gtagggcttg aggaaggggt
    17521 agaatcggtt aaacgagaac atgggtgtta ggacaatcgt ttcagagagg gcaggcctgt
    17581 agcgtatcac cgttggtggc ggcgatacgc atacgacatg accttcgtgg tgaggtgaga
    17641 tcgcgctaga gctgagcgat ggactcgatc aagttcttat tgatcgccac aaagtccgac
    17701 cgatccttat cgttgatcaa cacgtcagtc aggctgatga acagccgctg ctcttcgttg
    17761 atcgcgtcgg acacacgatt gcgcatgggc gggatgagga aatccccgac gtacacacag
    17821 ttcaccgttc gaacgcggat gcgaactttt ttgccttcgg gcacggatcc ctccgtccat
    17881 tatgtcgtga tgggctcagc tcttgcgacg caagaatttc tgacgccggc gcaacttttt
    17941 atacttgtgc ttgcgcatct tcttccggcg tttcttgagg acactcgcca tacattcaat
    18001 ctccaactgc taggcgcggg agtctagtgg gtttcagtcc aaaatgcaag ccaagattat
    18061 gacttgttgc atgtcgatag gccgcataat cgtatgcagg cggcgattga acgtggggtg
    18121 agccactcag cttgacctct ttccgccaag attcctatac tgcgccatga ttgaatcttg
    18181 cccgccgatt tctcttcgtc tcggtcgcgt tttccttgcc ctgctgctga tcatgactgt
    18241 gggttgtgca ggaaaaaccc ttcagtataa ggacgatcac gaccgtattc tccgtatcga
    18301 ccaggccgtg gagttgctcc gcaaatccta cgtagagcgg gaccgtagtg cgctggaggc
    18361 gttgctcctc ccgtcgggaa atctggatca ggtgcaacgt gagattcacg ccgacttcga
    18421 tacctttcgc gacattcagt tggagttttc catcgagcgc atcttaatcg aaggggacaa
    18481 tatcgatgtg tttgtccatt ggcagggcca gtggaaacgg gaccctgccg aggtgggcac
    18541 gcgccagcgt ggccacgggc ggttggcctg ggtcggcaca cagtccattc tgcttcggga
    18601 agctcagggc gatctgccct tcggcatggg cgcgcgcgcc agcgtgggcg aagacccggc
    18661 acgcgggcaa gccaagccat agccatgtct gccaggttgt cctcgtccaa aattgaaaca
    18721 gactttctcg tggtcggaag cggcgtcgcg ggtttgcgcg cggcgatcga actcagccgg
    18781 gccgggcgcg tgttgatgtt gaccaagggg catcctctgg aaagtaattc catgtatgcc
    18841 cagggcggcg tcgcggtggc gttgagcgag gaagatgatg tcggcagcca tttgaccgat
    18901 acgctgaagg ccggccacgg gttgtgtcga cgggaagccg tgcgtgtgtt ggtcgaagaa
    18961 gggccggaac ggatccagga actgatcgcc tggggcgcga agttcgacaa gatcggaaag
    19021 cgcttcgcat tcacccgtga ggcggcccac agccggagcc gcattctgcg cgcgcgaggc
    19081 gatgccacgg gcaacgagat ggtgcgcgcc ctcatgagct atgccgcgag gcagccgcgc
    19141 attcaacgat tggatcggcg attcacggtc gacctggcgg tcgtcgatgg acgctgttgc
    19201 ggcgctattg tgctgaatga gatgaccggc gaacgcacgg tgttaccggc cagtgccgtg
    19261 gtgttgtcga ccggtggcgc cgggcaggtc tatgcgcgga cgacgaatcc gggcaacgcg
    19321 acgggagacg gcatggcgat ggccctccgg gccggggcgt tgttgatgga tatggagttt
    19381 gtgcaatttc atcccacgtc cctgtatctg ccgtcgagcc cgccgtttct cctgtcggag
    19441 gccatccgcg gggaaggcgg ccagcttcgg aatatcaaag gcgagttgtt catgcatcgc
    19501 taccatccgg acggcgcgct ggcgccgcgc gatgtggtgt cgcgggcgat ctggtccgaa
    19561 atggcggcga ctcgcgcgcg tcatgtctat ctggatgtga cgcatctggg cgcggccttt
    19621 gtgaagcggc gattccccac catctatgcg acctgcctgc ggtacgacat cgacatgacc
    19681 gaagaatgga tccctgtctc gcccagcgcg cattacatga tgggcggtgt ctggacggat
    19741 gtgcagggag cgacgacgtt gcccggcttg tgtgcggccg gcgaggtcgc ctgcagcggt
    19801 gtgcatggcg cgaatcgtct ggcgagcaat tcgctgttgg aaggtttggt cttcggcatg
    19861 cgggctgcgc aatctgcggt ggcccatgcc ggacgattcc cgggaccggt gcgcatgccc
    19921 cacctggaag aactcgaaca gggccggccc acggacttcg acgaccccga gaagctgcga
    19981 agctcactgc gtcgcttgat gtgggggaag gtgggactcg tgcgctcagg cgattcgttg
    20041 atcagtgcct ccgcgcaact ggtgcgctgg atgcgcctgt tgaccaagcc atttgcctcg
    20101 cgagcggccc tggaggttaa gaacatggtg caggtcgccc gctgtatcac tgacgcggca
    20161 ttatggcggg agaatagtgt cggggctcac taccgttcag attttccgga gcccaatcgg
    20221 cctggatggc ggcaacacag ccagttgctg tttacggacg gtcagacgag cgggtcggca
    20281 gcgaaggagc gagcgctcgt gctttcgccc ccagcgcctg tgcgcggtag gtccgggaag
    20341 aagaaagccg gtcgttcgta aggaaggtgc ggtagtaccg aagcactttt tggacatact
    20401 gcctggtttc cggaataggg ggaagccctt ggtaacgctc cacggtatgt tcgccggcat
    20461 tgtatgccgc taaggccagc ggaagatttc cgttaaaccg gtccagcaac tgtcgcagat
    20521 atttcgtccc accgccgata ttctcatccg gattgtagga atcccgtacg tccaagcgca
    20581 cggccgtttg cggcatcagc tgcatcaggc cgattgcccc ggcgcgtgag accgcctgcg
    20641 ggtcgaagtc ggattccgtc ttgatcactg cgcgaatcag cgccggatgg agacgatggg
    20701 ctctggaatg tcgggcaatc accggctcca attccccgtc cgagatgacc gtgcgcgagc
    20761 gcgggagttc agcaatcact cgtctgtatc gaggatcgtt cggcacgtta gtgagagata
    20821 tggtgccgtt ggcatcgatg tactgataga cgtcagcctg gctggggagg gcattcagga
    20881 gcagtgccgc ggcaactgag aagagggctg ctgtgcggct gaaatgggcc atctggattg
    20941 tggatcccgt gtacgaaccg aatcccattc ggatgtctca accgtagcat cggacccccg
    21001 acctgtcaaa aaactccttc cctggctagc ccgattattc cctcataacc aattgatact
    21061 atggatatgt attgccatat tcgttacgag gtcggctcaa gaaacctcgt ccggctgtcg
    21121 gcgaatcgcg tctgtagccg acgggtcagc gagcccggcc gcccatcgcc gatggcgaac
    21181 gaatcgaccc tattgaccgg catgatttcc atgctggtat tggtcagaaa gcattcctcc
    21241 gcccgctgga ggtcagcgag gtgatagtga ccttcccgga cgggtacccc ttcctcttgc
    21301 gcgagggtca tgacgatggt tctcgtgatg ccgtccaaga tgccgcagtc cagggccggg
    21361 gtatggaggg ttttgccgga gatgaaaaag agattgctga cggtgcattc ggtcagctcg
    21421 tctttccaat tcaagaacag gctgtcgaag gtgccggccg caatcgactc acgcttggcg
    21481 agaatgttgt tgaggaaatt ggtggattta atctgaggag acagcgcttc gggcagattc
    21541 cgcctggtgt tggcgacggt cagcgacaca ccatcgcgtg agagtacggg aggagagggg
    21601 tggagcggct ttgccatgat gaccaccgtg gcccgcgggc acagaccggg atccaggccg
    21661 atttcccctg cgccgcgtga gacggtgatc cggaggtacg catccgtctg ttcgttgccg
    21721 actccgttcc ggcgcatcgc ttcgtggagc aactccggcc agtgcgactc cgggatagga
    21781 atgtccaggc cgatggcttc ggcggagcgc cggagccggg ccaggtgttg gtcgcgcatg
    21841 aagatgcggg ttccatagga gcgaagggtt tcgtagactc catcaccgta gagaaaccca
    21901 tggtcgaaca cggagacgag ggcttcttct ttgcgtacaa aacgatcatt gagaaacacc
    21961 cacatcagcg gccagcctcc agtattctga aaaatgcgcc cgctttggcg agcgtttcat
    22021 catattcgcg gtcgggatcc gagtcggcca caatgccggc accgacttgc agataccctc
    22081 ggttcttggt cagcacgagc gtacgaatga gaatgttcag gtccaggtcg ccgttccaac
    22141 tgaaataccc caacgccccc gtgtagagtc cgcgccgcac cggctccagt tcttcgatga
    22201 tttccatgca acggagttta gggacaccgg tgatggttcc gccagggaac acggctttca
    22261 caaggtcgag gggtgagacg cccggttgca gggtgccgac cacgtctgac accaggtggc
    22321 tgacatgcga atactgttcg atggtcatga actcatcgac ccgcaccgag ccgtagcggc
    22381 agacctttcc aaggtcgttg cgttcgagat ccaccagcat cacatgttcg gcgcgttcct
    22441 taggattggc aagcaactcc gcgcgcagaa gctggtcctg ttgcaaaccg gtgcctcgcg
    22501 gcctggtgcc ggcgatgggt ctggtgctgg cctgcgagcc ggtcagccgc acgagccgct
    22561 caggggagct gctgacgagg ctgaggtcgg cgaatcggac gagccccgcg aacggcgcag
    22621 ggttgatccg gcgaaggcgg cgatagagct cggttgagtc tccctcgaat ccggtcggtg
    22681 aagcggcggc gtgcagatcg agggtgaagc ggtgcgacag attggcctga tagatatcac
    22741 cggcggcgat atagtcctgg caacgccgca cacgagtttt gtaggcctcg cgcgattggc
    22801 tgggcacaaa ggtggtttga gtgggccagg gcgaggaggt aagaggcgtg accgggctcg
    22861 tgaggcgggc ttccagctca gccagccggt cacagccttc gcgatagagt ttctcccgcg
    22921 gctccgcctg aaatcgggac agaggggggc aatacatcag gtagagctgt cgcgtatggt
    22981 gatcgatcgc ggccacgacg tcgaaaaagg ccatgtgcag ttcagggaga tggagatcgt
    23041 cggcggccag ggtcggaagt aattcgaacg aacgaacgag gtcgtagctg aggtatccga
    23101 ctgcgccgcc ataaaacggc gggaggcctt ccggtttggt aatggtcgat tcggcgagcg
    23161 cttgttggag ggcctgaatg ccggaacctt gatagcgccg gatctgtccc ccggtctcga
    23221 tctggtattc ctgcgcgcgg cttgtcaggg tgagataggg gtcgcgtccg aaaaatgaat
    23281 agcgcgcggt ggtgtcggtg ccgttggcgc tttccaggag aaacgaggga cgttcggccg
    23341 gggccagccg acagtacaac tcgaacgcgt cgacatccgg ttgtggacgc acgaccacca
    23401 gcggttgcgg cggtccgctc aggaatgcct ggtgagagga aaagctcatg aacctgcgtt
    23461 tcagaggggt gccatacccg agcaaatgac tataccgcat cgagcggccg ggtcataggt
    23521 ggccaacttc ttgacaagcc cccaacgaat ttgcttgaat ggcgcgcccg ccgatgggtg
    23581 cactctctcc gattgcgctg aggagtgccg cacgtgagca tctgatgccc ccctctcagg
    23641 atccgttata tgcggggctc gggcaggctg tccggatcgg gacggaactg cttgctgcgt
    23701 tgatcgtcgg gggaggactg ggttgggtcg tcgacaccta tctgctcgat tctaatccat
    23761 ggggattggt ggtggggtta gggttggggg ccgcagccgg tgtgcggagc gcctatcggt
    23821 ctgcgcaacg atggcagagt taaccagaat cgtctgactg atatctgact gataaaggat
    23881 catcgtggaa gaaagtccgt tacatgcgtt tgaactgcac gatctgattc ccttggttcc
    23941 ggccggggtg gatatttcca tcaacaaggc cgtgattttg atgtgggtga tcgtcggcct
    24001 ggtggcgttc ctgatgatct cggctgcggc ggcccgtaag ttggttccgg gcaaattgca
    24061 gaacatggcc gaattgatcg tcgagttcat tcgcgggatt attttggaca ccatgggcga
    24121 gccggggatg aaatatttcc ccctgatcgc cacgttgttc ctctttattc tctttgccaa
    24181 tttgatcggg ttgattcccg gttcctatac cgtcaccagt cagatcattg tgacggcagt
    24241 ctttgctgta ggggtttacg ggctgagtat tgtgctcggc ttttcccttc atggcatgaa
    24301 gtttctcggc attctcgtgc cgcccgggac accgggttgg ctcttgccgt tgatgattcc
    24361 gattgaattg atcagtcagt tggcgcgacc catttccctg gccgtccgtc tgttcgcgaa
    24421 tatgactgcc ggacacgtca ttctgggagt cttgttcggt ctggcgatca gcggtgggtt
    24481 gctgatcggg tggctgccat tcgcgttcac catcgccatg aacggactcg aagtcggtat
    24541 cgcgtttatt caagcctata tttttaccgt gctgagctgt gtgtatctgg gtgatgcggt
    24601 taccctgcac ggtcatagcg agcacgcaca ctaggtcgag ttgtttacat aagggaggag
    24661 aggacaaaca atggatgcag cagcagcagc gttggtgggt atgggattgg cggcggcagg
    24721 atttgccggt gccggtgtgg gcatcgggta tatcttcggg aaaatgatcg aggccgtggc
    24781 ccggcagccg gaagcggaag gccgcgtcgg caagtacatg tggatcgggt tcgcgttggt
    24841 cgaagccatt gcactgtacg ggctggtcat tgcgttcatc atcatgggct tgcgtaagta
    24901 gtcgtttcga cggctgagcc ctgatgctgg ggcctattcc cggctcagaa cctcaactca
    24961 tttgggtaat agactatgcc tcaatttgaa tcacatttct tttcgtcctt gatcttctgg
    25021 gagattctct cctttgggat cctgttcttc ctgctctaca agtatgcatt tcccagcctg
    25081 ttgggcatgc tggaagagcg cgagaaaaag attaaggaca gtctcgatca ggcggaacgc
    25141 caccgttccg aagccgagcg caggctgaag gaatacgaag ccaagctggc gacggcggcc
    25201 aaagacgcag aggcgctcct ggcacaggcc aaagaacgcg cccaacgcct gatggaagag
    25261 aacgaacagc gcatgacggc ggatgcggaa cgcatcaagg gtgacgcgac tcgcgaaatc
    25321 gagaccgagc gcaggaaggc catccaggac atccgttcgc aaacgaccga cttggcgatg
    25381 atggtggctg agaaagttgt cgggcgggca ctgacggaag gcgatcatcg tcgtctggcg
    25441 gacgaagccc tcgatgccct cgcaaagagc taccagagcc ggaattagcc tctcttacgc
    25501 cgggggggtc agactcacgc ccccctggcg gtatccttcc aaatcaaccg tcacgaagcg
    25561 atagccgagt ttcttcaggg tttcggccag ccgttcccgg cgtcccggct gcaacaaccc
    25621 cagcagttct tctacggcca gttctattct ggcgatctcc ccatggtctc gcacacgata
    25681 ttggcgaaag ccttcttgtg cgagagcatc ttccgcacgc tccacgcgtg aaagtgtgct
    25741 tctcgtgatc gtgatgccgc gtggtatgcg ggaagagagg caggcggctg ccggcttatc
    25801 ccaattggag aggtgtagct ctctcgctac gtcgcggatg tcggccttcg agaatccggc
    25861 ttcgagcaac gggctacgga cgcctcgctc gcgagcggcc ttgaggccgg gacggtcgtc
    25921 tcccaggtca tccacgtttg tgccatcgac aatgtatcca tccgcatatt cgcttcggag
    25981 cgtttcgagg agtgagtaca agtcagtctt gcagtggtaa cagcgtgtgg catcattgag
    26041 gacgaattca ggaatttgga gttgatccgt ttcaacgatg cgatggcgtg cgccgatctc
    26101 tgcggcgatt ttctgcgtcg cgtccaactc actggccgga agggtgggag agacagccgt
    26161 aatgccgacg gcctgggcac ccagttcatc atgcgccaat ttgaggacga gcgaactgtc
    26221 gatcccgccg gagaaggcca cgatgaccga gcccatctct cgaaggatct gtcgtgcttg
    26281 agagacttta tcgatcagcg gcgtcgtgga aaccatggga ttctaggtct gcgcggcccg
    26341 gctgcgggag agtggccggc tattgtttga ctttggcctt cgcgatcgat ccgacgacga
    26401 ccaaggcgta atgttggggg tcgagatact gcttggcgac gcgtaggaca tcgtccttcg
    26461 tgaccttttc gatggcttta ggatactgcg tgaagtagtc caacccgagg ttgtagagtt
    26521 cgacctgggc gaggacgttt gccagtttgg cgctggaatc gacccgcagc gggaagctgc
    26581 cgacgatgaa ggacttggcc tcattgagtt cctgatccgt gaccggggca tcgcgcatcc
    26641 ctttgatttc cgtgaggacg cccgcgatgg cctggtttgt gacgtcggtt cgggtctgca
    26701 ggctgataaa aaaggctccc ggcatcaagc gggtgtcgaa ctggctcatg attccatagg
    26761 ccagtccctg tttgtcgcga atggaatcca tgaggcggga ggagaaccca ccggcgccca
    26821 ggatatagtt catgactgtg acggcatagt agtcgggatt ggtccggctg atgcccgtgt
    26881 gcccaaggac gatcgtcgac tgcgtcagat ccttctcgat gagctggacc attttacgtt
    26941 cgatcgaagc cggcttcttg atctggtagg gcgagggggg gccctttttc caggagccga
    27001 aatgggtctg gaccagagcg gcggcctgat cctgtgtcag gtctcccacg atgaccagga
    27061 tcgtttgatt cggcaggtat tccctggcgt gaaattgctg gatgtccgcg accgtaattt
    27121 tgttgagcgt ctcttccgtg ccatgggccg gccagctata gggatggccg tgaaaaatca
    27181 attggtggaa ggccttcatg gccacattcc cgggatcatc gtcatcgctg acgatttcgc
    27241 cgaggatctg cgtgcggacc cgttcgaatt cctgtttgtg gaacgcagga tgttggagca
    27301 tgtcggccag aagcgcaaag ccgaggtctg catccttctt gagcacgcgg gtggaggcgg
    27361 tggtgaaatc ctctgccgca tgggcttcca gcgagcctcc cacaaaatcg atctgttcgg
    27421 cgatttgtcg agatgtccgg gtcagggtgc cttcatcgag gaggctggcg gtcagattgg
    27481 ccagacctgc tttgtcgggt ggatcctgcg ccgatcccac cttgaccagg gcgtggattt
    27541 ccacggtcgg aagaaaatgc tgttccagaa acagcacggt catgccgttc gcggtcacgg
    27601 agcgggtggg gacgatgtcc gcagcttgcg ccaaggcgaa cggaaggctg agcgccatac
    27661 agagcaggcc cgctaacagc cggcccacag gccggcggct tcggtgttgg cgtggcagaa
    27721 gggtcgctgg ttgttgcgtc atcgggatcg ctcgctagtg aggttgtgcc ggtgacgcgg
    27781 cctgggtttg cggaggttga ggaatcaggg ttcccgtggt gcgcatatcg gcggccaggt
    27841 agtgcgacgc cgcacgctgc acgtcctgcg cggtgacggc gcgggtacgc tccacaaact
    27901 ggctgacttt gcgccatccg gccccgatcg tttcggcttc tcccagcagc atggcctggc
    27961 ggaaattcga atcctgttca aaaatgtggg ccgcttcgat ctgatttttc gcgcgctgca
    28021 gttcgagttc gctcggcggc tcggtttgga tccgctgaat ctctttgagc accgcgtctt
    28081 caaccgcttc aaccttctcg cctggtttca ccacggcata aaagtagaac aatcccgggt
    28141 cggcttgcat caggccatag tcggcgccga ctgccagggc ggtcttttgt tcgtacacga
    28201 ggctttgata gagccgggca ctcttgccgt gagacaaaat cgattcaagt acgttcaagg
    28261 cataggagtc gtcgctcgaa tagttgggta cacggtagcc catcatgacg aacggcacct
    28321 gcgcttctcg cttgaggaga aatcggcgtt cgccgtgttg cggggcttcc gtcacggcca
    28381 ggggtttggg ggacggacct ttagggatcg gttcgaagag cttggcaata gtcggaagga
    28441 gcgtgtccgc tttgatgtcg ccgaccacga tcagggtggc gttgttcggc gaatagtagg
    28501 tgtcgtagtg acgctggaga tcgtcgaggt tcatcgcgtc gaggtccgag aaccatccga
    28561 tgaccggcca gtggtacggg tgactcatga aggcctgtgc gaagagggct tccaccagcg
    28621 cgccttgcgg atcatcttcg ttgcgcaacc ggcgttcttc tttcaccact tcccgttcgg
    28681 tcttgaattc gttcgcatcg agaatcagcc cttgcatgcg gtcggcctcg agttcgagtg
    28741 cgagcgtcac gcgatcggca gccaggtttt caaagtaggc ggtgaagtcc tgactggtga
    28801 aggcattgtc cattccgccg ttcttgcgaa cgaggcgtga gaaggtgcct ttggggtact
    28861 tagccgtgcc cttgaacatc atatgctcaa gcatgtgcga caacccggcc cggcccatga
    28921 cctcgtttcg tgagcccacc ttgtaccaga cttgcacggt cgccacggga gccttcggga
    28981 cttccacgag gatcactttc atatcgttgg acaaggtgta ttccttgggc tcgacggcat
    29041 gggccgggga ggtctggctc gccaacagga tgaagcttag caatgaacag gcgaggatgg
    29101 agaaccgcac aggtgctggg ttggaagttg tgctctggtt aggcatgtac gaagaacatg
    29161 ctaacaacgg gtctgaatct gtgtcaaggc aacgtgggcc tcggacgtat cgaggcgtcc
    29221 gagctgaccg caagcgccta gcacatcgcg gccgcggctg cggcgaaggt aggcatccac
    29281 atggcctcgg cggagggtag cctgaaacgt ttcaatcgcc gcgtctgagg ggcgccgata
    29341 gggattgccc ggaaacgggt tgaaggcgat caggttgacc ttgcagcgga ggccacgaag
    29401 cagcttcacc aagcgggcgg catcctcggc acgatcattc acgtctgcga ggaggacata
    29461 ttcaaaggtc aggcgatcgc ggtccgccag cggataggcc cgacaggccg cgagcagggc
    29521 ctcgagcgaa tggagccggt tggccgcggg catgagttgc tggcggagcg tatcggtggt
    29581 cgcattcaac gagatggcca ggttcacctt gaggggggcc acgtccttga ttcgcgaggc
    29641 cagtcctgcg gtggagatcg tgatgcggcg acctgaaaac cccaagcccc aggtgttgtt
    29701 ggtgaggcgg gtgacggcat cggccacggc atccaggttg gcgaggggtt cgcccatgcc
    29761 catgaagacc aggttcgtca accgttcgcc ttcgctcaaa tggtcctgag caaggaggac
    29821 ctggtcgagg atctcatgcg cgcgcaggtt gcgttggagt cccagcgtac cggtcaggca
    29881 gaatccacaa tcgagcgtgc agccgacctg cgtggacagg cagagggtga gccggtcctc
    29941 gtcgggaatg agcacacatt cgacttgatt gccgtcggcc agcgtgagca caaatttctt
    30001 ggtgccatcc tgcgacgaaa agatttggac cgccgaagtc cgttcgatac tgcagctgcc
    30061 cgtcaggtac tcccggtcct tctgcgagag attgctcatc tcggcgaacg tgcgggcacg
    30121 ttcctgatag agccagcgca ggatttgcga cgcgcgatag gccggccatc cgagtgatgc
    30181 gacgaatgcg gccatcccgc tttcggtcac ggccagaaga ttggtgcgat tatcggtccg
    30241 ttggagcata ccggagccta gcacaggcaa aaaacttgtg acaagcaagg cgaacgtggt
    30301 cgcgtttata cagcgttcgg ctataatagg gccggttcac cctcttctca ggatgagaat
    30361 gtgaccgcac ttccttggcg gatcagcttg tcggctcttg ccgtgtggtc tgtgttgtcg
    30421 agcctgttct tgacggccac agccgagtca ccgcgccccc tgcctgttgc ccctgcaccc
    30481 tgcgtctccg cggaagattg tttccgttcc gccgtggcgc tcaatgaacg ttcgggatcg
    30541 cccgttcaac gcgatcaaac gatgatgctg aagatcgacc agctgcggtc ggtgatggag
    30601 ctctatcctt ccacgatctg ggccaagcgg gccggggtgg tgctgggcgt cttgcggatc
    30661 gagcgggagc cggtcgaggc ggcgaaactt ctgcgtgttg tccagccgga catgccggtg
    30721 ctcgacgact atctccgcct ctggatcggg gaatcgctgc tgaagcagaa cgaaccgatc
    30781 caggcggccg aattgctgga gacgattccc aagatcgtgc cggattcgag cctgattgcc
    30841 aaggctgcct accgaacagg cgaagcctgg tatagcgcca atgtgtgctt ccgggcggtg
    30901 gactggctgg agcgggctgt ggcgcttgcg gataaggacc cggcggctcc gttggccttg
    30961 tggcacctgg ccgagtgcta cattcgggag aatcgactgc cggaggcgcg aaccgcgttg
    31021 aagcaactct ggcttcgcta tccccactcg cctgaagcgc gggaggcgaa agcccggttg
    31081 gatacggcac tgggcggaga atcctggacc ccaacggccg aagatcattc aattcgcgca
    31141 caggcctttc tggggctggc catgcaagcg gaggcggtcg aagagctgcg ccgctttctg
    31201 gtgatggctc ccgggcaccc ccgccggttt gatgcccgct tgaagttggg cgtggcctat
    31261 gtccggctca agcaatatga ccaggcccgt gagacgtttc gttcgctagt ggcggatcgg
    31321 gtgcaggaat cgtccgaggc cacggtctgg ttggcccggg tctatctgcg gcagagtcag
    31381 ggcgacaagc ttatcgcatt ggcgcgatcg gtcgcgcagg gttcccttgg tggcgaccag
    31441 cgggcgatgg tgcacctctt cgccggagtg tggttggagg atcaggggca gttcgatgag
    31501 gcgatcggca tgttccggca ggtcgcgaaa ctgggggatt cggcgagtca gcgtgcggaa
    31561 ggtctctggc gtgccgggtg ggcgcaatat cggacagccc ggtatcggga cgcagccgaa
    31621 acgtttcgat ccgttgtgga gttgcatgtc aacggctttg agcctcaggc aatgtattgg
    31681 gcggcgaggg ccgacgaacg cgaaaaaaat accagcgttg ccgatcagta cacacgcctc
    31741 tgtcagcggc acgcctacag ttactactgc cagttggctg cacgacgagt ttcgttgccg
    31801 ccggtcatgc cggttgcggc agacgcggag cgtccggcag gcgacgaggc ttcacgattg
    31861 ccggagaatc ggcgtcctga gatcgaacgg catgtggtct atcaacgcgg aatcgagctc
    31921 aagatattgg ggttcgccca ggatgcggct cgcgaacttg gctctctcac agagcagtat
    31981 agtcgtgacc cggaggtgct gctggccttc tcgacgttgc tcagtgaagt gggagcgtac
    32041 catccagcct tacgcgtggc aaaagttcac tttaaggaga aactggaacg cagcggtttg
    32101 ccgaccgcgc ccgccttgtg gaccgtggcc tacccgaccg gcctgctccc gacgatcacc
    32161 gcgcagggtg tcgccgcggt tgacccccac ctggccgccg cgatcattcg cgaggagagc
    32221 cagtacgatg agaaggctgt ctcgatggtg ggagccgtgg ggttgatgca gttgatgccg
    32281 gtcacggcga atgccgtggc acagcggtac gggtttcccg cggtcggacg ggaagaactg
    32341 ttcgaccagg aaaccaacat tcgactgggg gtgcggtatc tgggacaatt gctcgagcaa
    32401 tacagcggga atctggccca tgccgtcgcg gcctacaacg ccggcccgat tgccgtgaac
    32461 aattggatcg ccgtgcatcg aggacgggag caggacgagt tcgtggaact cattccgtat
    32521 caggaaacgc ggttgtacgt gaaacgggtc ttgcgcagct atggagaata tcgccgtctc
    32581 cataacggca cgtcgtagcg gggccgtttt cttgacaagc cggagcgaac tttctatatt
    32641 tcgcccccgt cacccaacgt gttcgaccac cactttgata tgaggaaata ccatgacgtt
    32701 agatcatctc gacgcccttg aaattcggat tcgcgatttg gtgaagctgg tgcaggatct
    32761 caagaggaaa aatgcctcat tagaggatga cttgcgtctg gcgcgggagc gggttgcggt
    32821 tcgtgatgac gaaaaccgac gctgggaaca ggagcggatc gatatccgtt cacggatcga
    32881 gaaagtcctg ggcgagatcg atctgttgga atgcctggat gaacccaagg aggtggctgt
    32941 tgactaagac cattgacgtg gagatctatg gccagcggta cagcatcaac ggggaagcgg
    33001 acgaatcgta cgtgaagcaa ttggccgata tggtcgataa gcagatgaaa caggtggcgg
    33061 ccggcatgcg gtcggccacc ccggccaagt tggccgtcct tgccgcattc aaccttgccc
    33121 acgaattgat ggaatcagag cgaaagtccc ggcagggaga ggccgacgcg gatcgtcggg
    33181 tggcctccct gatggaatcg atcgatcagc agatgccgtc catcctgtca cggtgagttt
    33241 cgccttgctt tcactgtact gctttgttat cgtttctgtg ttgtattgac ctgagggtca
    33301 acaagagcag gagaggaaag gaagcttcga acaaggacgg aaaatctgac gacggttttt
    33361 gagcgctaaa catatggaat cgctggtttt tgtgctgtta ttcacagtgg tcgtgatggt
    33421 gtggatgaga gaaggctccc aggctggcat gtctgttcga caggtgtgtt cgcaacgtgt
    33481 agcaaggggc gaagagcagg tgctggcgga tgggctatgg cctgcgctgt gctcgcaacc
    33541 tgtgacgatc cccgaccggg gggcttggtc tgtgcgcctt cgatgtgaca gacgtattga
    33601 ttcgggtcga agtcgaccac tcctccacgt gaattgacgg cggcgattcc acgcttcgtt
    33661 tctactggct caagaagtca gatgtttccc cctcacgcgt agacattcct ttctcacgct
    33721 ccttgctctc cctttaacac tggctgtgac tattcgcagg gtgacagtgt ttactgtccc
    33781 gctgccgtaa gggaggtggt tcccatttct ctcagcgttg ttgcgtacat ttttgtcgga
    33841 ttagtgggag cggtcctggg tgccgggttg tatgaggtct ttcgccgtcg gtcggcgttg
    33901 gctcgtcgtg ccgaggccga ggatcagtcg gcgcagatcg ttcaatcggc tcagcgtgaa
    33961 gcagagaatc tcgtcaaaga ggccaagctg gaggccaagg atctggtctt tcaggccagg
    34021 atcgaactcg aaaaggaaca aaaggccaag ctggccgaag tttccaattc ggaacggcgt
    34081 gtgtcccaac gggaagaggg actcgatcgc aagctcggct tgcttgagaa gcgggatcag
    34141 gaagcgctca agcgtgagca ggacttattg aagcgtgagg agacactggt acagaaggac
    34201 gcggcctgcg cgcaggcgct gaaacagcat cgcgaggccc ttgagcgggt ggccggcttg
    34261 acggctgagg aagcgaagcg gcaactgatt caggatctgg atagtcaggc gcggttggaa
    34321 gcggccggcc tggccaagcg cctgctcgaa gaggccaagg aaaatgccga ccgggaggcg
    34381 cgggaaatca tcgccagctc gattcaacga gtgacccggg attatgtgaa tgaagccact
    34441 atctccgtgg tgccgattgc caacgatgcc atgaagggcc ggattatcgg tcgagagggg
    34501 cggaatatcc gggcgatcga ggcggcgacg gggatcgacc tcattatcga tgaaacgccg
    34561 gaggcggtta ttatttcagg gttcgatccg cttcgtcgtg agattgccaa ggtctcgctt
    34621 gagcggctca tgcacgacgg ccgaatccat ccgacgcgca ttgaagagat cgtcgaaaag
    34681 gtgaaggtcg atatcgagaa gctgatgatc gaagaggctg aaaaggtcat cttcgaggtt
    34741 ggcttgtctg attttcatcc cgagttggtc aaggtgttgg gacggctcaa gtaccgaacc
    34801 agctatgggc agaataatct gtaccatgcg cgggaagcgg cctacatctg cggcatcatg
    34861 gcctccgagt tgaaactcga cgtgaaactc gccaagcgcg gcgccttgtt acacgatatc
    34921 ggaaaggccg tcagtcacga agaagaaggg ccgcatgcca tgctgggtgc ggagatcgcc
    34981 aagaagtacg gcgaaaacgc caaagtggtg aatgccattg cggcgcatca cgaacaggtt
    35041 gagcccattt gtccggagac cgtactggtg gcggcggcgg aagccttgtc ggctgcgcgg
    35101 ccgggggccc gtcgtgaagc gttggagtcg tacgtaaagc ggctggagaa attggagtcg
    35161 ctggcgaccg ttcacaaggg tgtgcagaag gcctatgcga ttcaagcggg tcgtgaaatt
    35221 cgtgtcattg tgaagcagga agatttgacg gacccggaat gtttccagtt gtcccgggat
    35281 ctggcaaaga agattgagca ggagctgacc tatccggggc aaatcaaggt gacggtgatc
    35341 cgggagagcc ggttcgtcga ttttgcgaaa tgagcgaacg gttatgaagg ttctgatgat
    35401 cggcgatatc atgggcgagc cagggcgtcg gtccgtggct cgcctcctgc cgaaactgat
    35461 tgccaaccat tccatcgatg ttgtggtcgg caacggcgag aacgtggccg gcggattcgg
    35521 tattactccg gacctggtgg acgatctttt cgatctcggg gtgtcggtca tcaccacggg
    35581 gaatcatgcg tgggacaaga aggaaatcct cgatgtgttt cctcgtgaac cgcgtctctt
    35641 gcgtcctgcg aattatccca ccggagtgcc gggccgaggc agttatgtgt ttaccacgcc
    35701 cggcggcgaa tcgttgggtg tcctgcattt gatggggcgg gcgttcatgc cgacgatcga
    35761 ctgtccgttt caggtcgcga agcgggaaat cgaacgtctc aagacgcagg tctccgcgat
    35821 cgtggtcgat atgcatgccg aggcgacgtc tgaaaagatg gcgatggggc attacttgga
    35881 tgggctggtg acggttgtgg cggggacgca cacccatgtg cagacggccg atgagcaaat
    35941 ccttccgaaa ggcacggcgt acatcacgga tatcggcatg accggcccgc ttcattcggt
    36001 gatcggaatt aaaaaagaac tggcgatcga aaagtttctc accggaatgc cgcggcgctt
    36061 tgaagtggcc tcgggtcccg tcgtcttctg cgcggtattg atggaactgg atgccacgct
    36121 cggcaaggct ttgtcgatcg aacgaatccg agtggtggat tgatctgttc gcctagccat
    36181 tgtctcaggc tcggtaagct atgaccggcc aaacccttcc gctccctctg cttctttccg
    36241 tatccgacgt gacgcgactg atccgtgatt ccctggaagc gcaattccgg gatatctgga
    36301 tcgagggcga aattaccaac cttcgcgcac cgtcttccgg acacctgtat ttcacattga
    36361 aagatgagca gagccagctt cgcggggtgc tctttcgatc cggggcctcg cggcttcggt
    36421 tcaccctgca ggagggcctg gtgatcgtcg cgcgcgggcg catctcggtg tatgagccgc
    36481 gtggcgagta tcaactgatc gtcgactcgc tggaaccgaa gggcgtcggg gcgtttcaac
    36541 tggcgttcga gcaactcaag gagcggttgg cgcgagaagg attgttcgac gaggcgcgaa
    36601 agcgtcccct gcctcccttc ccacgcacgg tgggcgtggt gacctctcgc accggggcag
    36661 cggtccgcga tattgtggcg gtcctccgtc gccgctgtcc ggtcgcgaat attctcatcg
    36721 cttccgtgcc ggtccagggc gagggagccg ccgagcagat tgcggaggcg atcacgacgt
    36781 taagcggaat gccccaggtt gaggtgatga tcgtcgggcg tggaggcggc gcctcagagg
    36841 atttgtgggc gttcaacgaa gaagtggtcg ttcgcgccat tgtgcagtcg agggtcccgg
    36901 tcgtgtctgc ggtggggcat gaaatcgatg tgacccttgc cgactttgcc gcggactatc
    36961 gggctccgac accctcggct gcggccgagg cggtggtgcc ggtgttggac gagatcgtgg
    37021 agcgactagg cgagatgtct gatcgtctgt atcgagtcgt gcgcactttg ttggagatgc
    37081 agcgccatcg gttcgagcga tcggttggag tgatgcgcga catgcgcttt cgagtgcagg
    37141 cgcatgccca gcatctcgat gaattgcgag acggattgac gcgcacgctg accgaacgat
    37201 taactctgct ccaccggggg atggtggtgc ggcaacatgc cttgctggct caaggaccgc
    37261 acaaccggat tcagaccgcg ctggtggtga tcccgcaact gtataaacgg ttggagcaag
    37321 aagccagacg gggacttttg tccaggcggc aagcggttgc gtcgtacatg atggctttgg
    37381 acgctctcag tccattggcg atcttaagcc gcgggtatag cgtcatccag gcaattccgt
    37441 ccgggcggat cgtccggctg gcgtcagacg tggcggtggg ggatgtggtg caggcgcggc
    37501 tggccgaggg gcgtctgttg tgtctcgtca gtgaggtgct gccgccttcg atgccttgac
    37561 ctcaccggtg ggacaggcaa taatgttctg tttgatgaat gagcaaggga gttgttgtgg
    37621 ctgccgtgaa gtttgaatat gcaatggcac gattggaaac gattgttgcc gaacttgaaa
    37681 agggcgatct tcctctcgat gactcgctga agattttcga ggaggggatt cggttgtcca
    37741 agacctgcct gaagatgctg gaggatgccg agcggaaggt tgaaatcctg gtgcaagaga
    37801 aggacggcaa gaagcggatt caagcgtttt ctcccggtga ggatgagccg gagcgccctc
    37861 agtaacggtc tgatcgatgc acgggctttc acgttgcccg tgtctgtcgc aattaaactt
    37921 ccatcacagc ctatgagcca gaaattacgc ccagagcggg aacggctgga tcgcgtgctg
    37981 gtcagccgag ggttggttgc cagtcgggaa gacgcggctc gactcatcct ggccggcctc
    38041 gtccgtgtgg atggggtggt ggttgataag gcggcgaaac cgaccttgtc ggatgcgaag
    38101 gtggagctga ccgggccggg gtctccctat gtcggtcgcg ggggcgagaa gttagccggc
    38161 gcgctggatc aatttcaggt cgatcccaaa ggaatgatgt gcttcgacgt cgggtgttcg
    38221 accgggggat ttaccgattg tctgctgcag cgcggcgccg cgcgtgtcta tgccgtcgat
    38281 gtggggtatg gtcagtttga gtggcgccta cggcaggatc cgcgcgtggt gctcatggag
    38341 cggaccaaca tccggtatct tgagccgagt gcgatccggg atccgatcga tctgattgtc
    38401 atcgatgtgt cgtttatctc gctgaccctc gtgctgccct gtgttgtgcc ctacctcgct
    38461 gcgtcggggt tcgtgatcac gttgatcaaa ccgcagtttg aagtggggaa aggtcttgtc
    38521 ggacggggtg ggattgttcg tgatgatggc ttgcgggaag gggcggccga caaagtggtg
    38581 gcgtgcgcgc agcagttggg gctggagttg gcggatcgaa tggaatcgcc catcgagggc
    38641 cgaaagggca atcgggaaat cctggcctgg ttccggcgca gggcgtgagg ggtacgagta
    38701 tggatatcag tgggtctgcg gcgggtttcg tccgggacgt tcacgcgcaa gggcaacacg
    38761 aaaacacttt gcggaagcct gcagaaggtg taggctgacc ctcaattgtt cggtcagggg
    38821 atcggcacat ccgcagacga aggagaggcg aggagacgta tgaaagtctt ggtcacgggg
    38881 ggcgcagggt ttatcgggtc acatgtcgtg gatcgtttgc tccaggaggg gcatgatgtt
    38941 gtggtggtcg ataatcttgt cacgggaaag cggaaaaacg tccccaaggc ggcccaattc
    39001 tacaaactgg atatagaaaa tcccaagctc gaacggattt ttcgcaacga acggccgtcg
    39061 atcgtgttcc acctcgccgc tcagatgaac gtgcggcgtt cggtcgaaga tccgatgttc
    39121 gatgcgcagg tcaatgtgct ggggacgctc aatgtgctgg agcaagcgtc gaaacatggc
    39181 gcgcgcaagg tcatcttctc ctcatccggc ggggcgattt atggcgagca gctggcattt
    39241 cctgccccgg aaactcacat tacccagcca ctgtcgccct acggcatcag caagttatgc
    39301 ggggagcatt acctatccta ctaccaccgg ttgagcggta ttcaggtggt cagtctgcga
    39361 tatgccaatg tgtacggccc gcggcaggat ccggaagggg aggcgggagt cgtcgccatc
    39421 ttcattcaaa agatgctgcg tggcgaacag gcggtggtca acggaaacgg gcgtcagact
    39481 cgcgattttg tgtttgtcga ggacgtggtg gaatcgaatt tgatggcgat gggtcccgag
    39541 gtggagggcg tgtacaacgt gggcacggga atcgagacgt ccgtgaacga cctcttcaaa
    39601 atcgtcgtcg atctaaccaa ggtagagttt aaggaagtgc acggcccggc caagcgaggc
    39661 gagcaagcca gaagcgtcat cgactccaca aagctccacc gtgaccttgg gtgggaaccg
    39721 aaggtcgatc tgcgcgaggg actgcggcgg acggtcgaat attttcgcga cgggctcgga
    39781 tagccgcttg agctaatagc ggggcacgga tgggtctacc tgagtagacc aggcatcaat
    39841 gcctccgatc aggtttttca cctttccgaa gccttgctgc agcagaaacc ctgtcgcatc
    39901 cgcacttctc atcccgtggt ggcacacggc gacaatctcg gcgtcacggt caagtttgcc
    39961 aagagattgc tgcagggtcg ccagcgggac caggatggat ccctccaact tggcgatggc
    40021 atattcccat ggctcccgga cgtctaccag caccagcttg tcgcctttat ccagcctcga
    40081 cttgagatcc ttaggggtaa tcgtgaaact catcagcgca cctccatcta tacggtcgga
    40141 tcataaggag ccgaaaaatg ggtgtcaact ccggtgtggt cggccattcg tcgcgtgggg
    40201 tctgatctca agactttgcg gattcagacc gaagcgtatg gatgagagga cgagggcctg
    40261 ggcctgacag tcggtcggca ggtaacgcct ctggagaatg gtcgtttttt gtcgaattgt
    40321 gcaaatcctg cctagttttt taagcgtaat cggcacggag atggggtaga attctgccct
    40381 aagtgcttga taactgaagc gcaaacagta ctcgccgttc tgtgtcggca cgcaatttgc
    40441 ttagagctgt gggggagacg tgggagcgaa cggttcagag gtagtggtgt ttgtgactgc
    40501 ggcaacggcc gaggaggcag aaagactcgg tcggatcatc gtggagtcca gacttgccgc
    40561 ctgcgcgaat gtcttaaatg gcattcgatc gattttccgt tgggagaata agatcaacgt
    40621 cgaaaatgaa tgcctgatgc tcattaaaac aaccctggag cgatacccgg aactcgaagc
    40681 agtcattcga cggcaccata gctatactat ccctgagatc atcgcactcc cggtcattgc
    40741 gggatcggcg ccctacctgg aatgggttcg ggaagagact cgtaagtagt tgattctaat
    40801 aatgtaattt ttgaaaacaa agggttgacc atcgcgctag agatcagtac actgcaaaag
    40861 tcagtggctg aattcttggt tgaatccgtg aaggggagag cgtcctatga gccaacaagc
    40921 ggccgaaaca agtgatgcct atactgtggt ggtctttcgg gggtcctcct ccaagccgtt
    40981 acggtttagt tttccacgga aattcgtgcg caagctcctg atcttggctg cgatcctggt
    41041 cgtggcagat ctgctcgtta tctctcatta tgtgattcgg accggagaag tctggcaatt
    41101 gtcggcgttc cgggctgaag ccatgggtgc gcgtgagcaa accgctgcct tctccgctgc
    41161 gattgatgat ctgaaaaagc gtctctcgac catgggcgag gtcaatcaac ggcttcgagt
    41221 catgctggga attgacgcca gcaagccggc cggggatttg gcgaatggac ggggtggcga
    41281 ggacggtccg ctgccggatg gaaaaaccag tgtgcagggg aatggatcag ccacttctgg
    41341 gctggaaccg cggcagcagg tctccgaagc gcgtgatgcc aatcagtccg aggttgattt
    41401 tgctacggaa agtattgaag aggtcactca gcaggttcgc gaaagccttg aggccctcgt
    41461 ccgggaggcg aagcaacaag aggaagccct cgaaagcctg acccaagttg cagagcagcg
    41521 ttcgactcag tgggcgtcaa ctccctccat ctggcctgtg cgcggatggg tgacctcggc
    41581 gtttggcccc cgggtttcgc cgttcaccga aaagccggcc tggcatgacg gtctggatat
    41641 cggtgctcag gcaaattccc ccgtccaggc tccggcgctc gggcgtgtcg tcaccgtggc
    41701 ctttgactcg aaaatgggca atatggttaa gctggaccat ggatatggga ttgagacggt
    41761 ctatggacac cttgccaagt cgttggtgaa agaagggcag cgagtgaagc gcggtgatgt
    41821 ggtcgcgctt gttggcagca ccgggctttc cacgggaccg catctccatt acatggtcaa
    41881 gaagaacggc caagcgttgg atccgaccaa gtttattctc gactagtctt ttcgcattcc
    41941 ttccagattt cgaaagaagt ttgcctgttc ccttcctctt ttccgttgta atcctcgact
    42001 acccgcgtcc cagtccagct ctaccagccc tgtgctcccc agtctttgcg gcccattact
    42061 tcggcgaatg ccgtggtata ctccgcactt tcctgaaatt cctgttctca cgaggaacat
    42121 tgcatgactg atctagagat agcccgttcc gtccctcaca agcatatatt cgaagtcgca
    42181 cagtccttag gcattcattc tgacgatctc attccctttg ggcgctacaa ggccaagatc
    42241 gcgcccacgc tgggtgagcg aatccagaac agaccgctgg ggcgatacat cctggttacg
    42301 gccatcaatc caacacccct tggcgagggc aagactacga cctcaattgg gctgagcatg
    42361 gggttgtgcc gcttaggcca tcgggccgcc gtcacgctca ggcagccttc cttgggaccg
    42421 gtcttcggaa tcaagggggg cggtaccgga ggcgggagag cccaggtcca cccgatggaa
    42481 gatatcaatc tgcattttac cggcgatgcg catgccgttt ctgcgagcca caatctcctt
    42541 tccgcatttg tcgataacca tctgttccac ggaaatacgc tcaaggtcga tccgaaaggg
    42601 atcagatggc cacgcacgct tggggtgagt gaccgcgcgc ttcgcgacat tctcctggga
    42661 gaggaaaccg ggcggcggcc ggggaagttt gtgattacgg aggcgtcgga agtcatggcg
    42721 gtgctggccc tggcgaccga ccatgcagat cttcgccaac gcctcgggag aattctgatc
    42781 ggcctgactg agggcggaca gatggtccgt gctgaggaat ttggctgcgc cggctccatg
    42841 gccgtcttgc tgaaggatgc cttgttgccg aatctcgtgc agaccctgga gggaactccg
    42901 gcatttgttc atgccgggcc gttcggtaac atcgcgcatg ggaattgctc gattgtctcc
    42961 gatcggttgg cgttacgttg tgcagactat gtcgtgactg aagctggatt cgggagtgat
    43021 ttaggcgcgg aaaagttctt caatattaag tgccgcacgt ccggtcttcg tccggatgtc
    43081 ggggtggtgg tggcgacctt gcgtgcgctc aagcttcatg gcggtggggg aatcgtgaaa
    43141 tccggtgctc cgctgcctcc cggtgtgacc gggccgaatc aggcggcgct ggagcagggc
    43201 tttgccaacc tcgagcagca tatcgccaat gtccgggccc atggagtgcc ggtggtggtc
    43261 gcggtgaatg cgttcaagga tgattcaacc gatgaactaa actgggtgtg cgaacgcgcc
    43321 ctggaagggg gtgcgtcggc agctgcggtg tcgacccatt ggtccgacgg tggacgggga
    43381 gccgaggagc tggccagggc ggtcgtcaag gtcgctgcgc aaggtacgca attcagtcct
    43441 ctctatgagg tgacctcgcc cattaagaaa aagattgaga ccatcgccac tacgatctat
    43501 ggagcggccg gcgtgtcatt ctcgcctcag gccgagcggg acgtagagct ggcgacacga
    43561 ttgggattcg atcgacttcc catttgtatg gcgaagaccc cgctttcact atcgcacgat
    43621 ccggcggtga aggggcggcc ctccgggttc acggtgccga ttcaggagtt gcggattctg
    43681 gccggggccg ggtttctcac cgcggtctgc tcaggcattc agttgatgcc gggtctgccg
    43741 aagaagcctg ccggggaacg gatcgatgtt gatccacaat cgggtcaaat tgttggcctg
    43801 cagtagtggc gtgttaccgc tgtttcaggt accggaagaa ttctgattcg ggactgacga
    43861 ggatggtggt cttgtccttg agggtcttgc gataggcttc catggtgcgg gtaaactcga
    43921 agaaatgcgg atcctggcga taggcatcgg catagatgcg gaacgctttg gcgtctccgc
    43981 ctccgcgcaa ttcctctgat tctcgatagg cctctgcgag gatgatctcc cgatctttct
    44041 ccgcctccga cttgattttc tgagcctctt ccgcgccttc tgcgcgatac tgcttcgctt
    44101 gtcgctcccg ctcggcttgc atgcgcgaga acactgcctt ttcattctgc tcaggcaggt
    44161 cggctcgttt aatccgcacg tcctggattt ctatgccata ggcagacgct ttttcgttgg
    44221 cgcgttgggt gaccacggcc atgagttgcg cacgggccga tgagacgatt tccgccagat
    44281 cgtgccggcc taattccacc cgcagttccg aataaataat atcgtgcagg cgctggagcg
    44341 cgccgcgctg gctttggaac gcctgataca ccttcagcgg gtcggtgatc cgccacttcg
    44401 caaaattatc gagcagcaag gtcttcttgt cctgggtaat gacgtcctgc gcattggagt
    44461 cgtagtccag aaggcgcttg tcgaagtagg tgacttcttc gatgaacggc attttgaggt
    44521 agagaccccc ttcggtgacg ttccggaccg gtttcccgag ctggactacg atggcattct
    44581 gggtgacatc tacgatgtaa aaaggcgatg caccgaggac gagcagcccg agaacaatgc
    44641 cgacaaatgc caggataaat ccttgcttgc tcatggcgtg cgctcctgtg tcgaactgga
    44701 cggggccggt ttggagaacc gatcgagcgg cagatacggc agcgcacggt ccgccccttt
    44761 gccgtccagg acaaatttat cgatgtgggg gagcacgtct tcaagggtct cgatatagat
    44821 ccgcttgctg ataatgtctt tcgcctggtt gtattctttc agcgtcgcga ggaatcgatt
    44881 ggattcgccc tgggagcggt tgagccgcgc ctgcgcatac cctttggcct ggttcaccaa
    44941 ctgggccgct tcacccttgg ctttgggggt aatatcgttg cgatagccct gagcttggtt
    45001 gatcagcttt tcacggtctt ccttggcgtt ggtgacgtcc ttgaacgcgg cggccacggc
    45061 ttcgggcggg tcgacatcct gcaactggac cgcggcaacc tgcacgccgg tcctatagtc
    45121 gtcgaggatg tgttggagaa gttcctgcgt gtcgttctgg atttgtgctt tgcccgtagt
    45181 gagagcttca tcgattttgc tcttgccgat cacttcccgc atagaggctt cagccgcctt
    45241 cccgatggtc tcgtcgatat cggccacatt gaagaggtac tcgcgcgaac ttttgatttt
    45301 atattggacg ataaattcaa ttgccagaat gttcatgtcg ccggtcagca tcaaggcttc
    45361 ttgcggcacc atctgctggc ggccttggcg atcctttcga aatccgatct ccacccggtg
    45421 aagcttcgcg accttcggtt gcaagacgct ttcgatgatg ggaatcttca tgtgtgggcc
    45481 cggatccaca acgcgcacgg ggataccgaa gcgtttcacc acgccttctt cgtccggtgc
    45541 cacaataaag gcgctttgcc agatgaggaa gaccgtgaag gcgacaagaa gaagattgcg
    45601 gaaaccgccc gtaggtaaca gattgcgaag ttggccctgg gcctgcttga aggcctgatc
    45661 caaatcgtcg cccttcttgc tccaagggtc tttggggtcc cagaccatgt agtggctcaa
    45721 ttcagtgagg gtggcaatgt cgccagcgaa tcaccttagc aaagtgagga ccgtcgagca
    45781 acacataaag gcggccggtt gacttatcaa gattcgttga tatatatttt tgtcatgaag
    45841 ggaagcacgg tgcgaaatcc agcgcgggcg gcggagttgt ttcacgcgct tgcggacccg
    45901 acgcgattgg agattctgga cgagctgaaa gagggcgagt gctgtgtgtg cgaactgacg
    45961 gatcgcctgc aggccgggca gtcgcggctc tcctttcatc tgaaggtact taaggacgcc
    46021 ggaatgatct tggatcgacg agagggccga tggatgtact actccgtgaa cgccgacgcc
    46081 cttgccgagc tcgatgacct cgtcgattcg ctcaagcagg cgaagcgggc gcgccgcgcc
    46141 accggctgtt gttgattttt ttccgcctaa tagatcaatg aatcttgatg cataaaagag
    46201 gaggcgacga aatggacgca caagcgattc gagagctggt caaggagaaa tacggtgaag
    46261 cggccgctag agccaagagc ggaggcagtt cctgctgcgg ggcctccccc gcgctgatga
    46321 atgtcgatcc gatcacgtcc aacctgtatt cagatcagga gcgtcagggg ttgccggcgg
    46381 atgccgtggc ggcctcgctg ggctgcggga atccaacggc tctggcagaa cttcatgccg
    46441 gtgagacggt gttggatctg ggatccggcg gcggcatcga tgtgttgctc tctgccaagc
    46501 gagtcggtcc ggcggggaaa gcctatgggt tggatatgac ggagcagatg ttggcgttgg
    46561 ccagagaaaa tcagcgcgca gccggcgtgg agaacgtcga gtttctgaag ggcgaaatcg
    46621 agcacatccc gctgcctgat cgttccgtgg atgtgatcat cagcaattgc gtcgtgaatt
    46681 tgtccggcga aaaggagcgc gtgctggcgg aagcctttcg cgtcctgcgt ccgggaggcc
    46741 ggctggcgtt gtccgacatt gtagtgcgag gggcgattcc ctcagagatc cgacggaatc
    46801 tcgaattgtg ggctggttgt gtcgcgggcg cgttggaaga gacggagtat cgggacatgc
    46861 tggcccaggc ggggttcgtc gagatcggaa tcgagccgac gcgcatctat caggccgatg
    46921 acgtgaagga attgctggtc ggcacagacc tctccagtga tctgctcgtg gcccaagtcg
    46981 agggcaagtt catgagcgca ttcattcggg ccaagaaacc ggccgtgacg gtctaactcc
    47041 gccgggcagc gcgagcacga gggagcagga gcgatggaac tcgcattatc ggtcgatgag
    47101 ccgcaggtgg ctacgaagcg gctgtcgttc tttgagcggt atctcacgct ctgggtggcg
    47161 ctctgcatgg tggccggtgt gctgatcggg caggcggtgc ccggtttggt gtcgagcctg
    47221 cgaggggctg agatcggaca gggcagccac atcaatctgc cgattgccgt gctgatctgg
    47281 ctgatgattg tgccgatgat gatgaaggtt gagtttgcct cggttcgtga cgtgggcaag
    47341 cggccggtcg gcctgctgat taccctgttt gtgaattggg tggtgaagcc cttctccatg
    47401 gcgttctttg cctgggtgtt tttccggtac gtcttctcag cctggatctc accc
//
LOCUS       DAORRQ010000033        47148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_48_length_47148_cov_43.888370, whole genome shotgun sequence.
ACCESSION   DAORRQ010000033 DAORRQ010000000
VERSION     DAORRQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 47148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47148
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48_length_47148_cov_43.888370"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PK224_11635"
     CDS             complement(<1..433)
                     /locus_tag="PK224_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HRB16433.1"
                     /translation="MGDDLIKYLGPLAALAGVWEGDKGADVAPDDNRGTEQNAYRERL
                     TFEPFGPVNNHEQQLYGLRYATVAWRLSDGAPFHEETGYWLWDAAAKQVLRCFIVPRG
                     VTVLAGGTVEPGAVSFQISADAGSDTYGICSNKFLDREFKTV"
     gene            complement(549..899)
                     /gene="ssrA"
                     /locus_tag="PK224_11640"
     tmRNA           complement(549..899)
                     /gene="ssrA"
                     /locus_tag="PK224_11640"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1093..1707)
                     /locus_tag="PK224_11645"
     CDS             complement(1093..1707)
                     /locus_tag="PK224_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16434.1"
                     /translation="MTRLVVLMIAGTVLMSSGREAGAWPELSTTVSEAMVWLTKGSSR
                     SKPAPLKYKVVGVFQRNKQFVGGSAVADVTKQVEFEFKWPLEDGVSPKVLPDIPTKAE
                     HYRDGSAVCPAPILKGAYEHFELKSILVMGPMFGLVGERRTPDMEEYVGGGGCKGTRL
                     VQGQVREEQVLVTLPPEFLAGQLSKGQVFTQQDQGWTWTFTPVP"
     gene            complement(1954..2904)
                     /locus_tag="PK224_11650"
     CDS             complement(1954..2904)
                     /locus_tag="PK224_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HRB16435.1"
                     /translation="MNTFQFLADHGASVLFWVIFVEQIGFPIPAIPLLIAAGALVGAG
                     KMSLATALLVPVAAALPPDLAWYYLGRIKGGKVLGFLCRLSLEPDSCVRDTENLFHRY
                     GPRGLLLAKFIPGFNTVAPPLAGIVGMAATTFALYDVAGTLIWAAVSAGVGALFSNQL
                     EQLVGLLDQAGGLVFGVLLIGLAGFIGYKFYYRQQFLRHLRMAKISVDELKQRLDAGE
                     AISVVDVRHPLSLQLDPDTIPGAINFTLEEIEHRHQEIPRDRDIVLYCTCPNEVSSAR
                     TAFLLKKKGIHRVRPLEGGLDAWRERQYPVERRVNAGVGG"
     gene            complement(2925..4550)
                     /locus_tag="PK224_11655"
     CDS             complement(2925..4550)
                     /locus_tag="PK224_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16436.1"
                     /translation="MIAAASRWIDRNILELARDFQLSYLPPLMVYMAAGISGLTGIVG
                     TFFVKDYLGLSAAFLAALGFWAGIPWALKMPFGHLVDLLWRWKSLLVYFGAGVIAASL
                     LIMVGLIGHREAMVAVMPAEVWYMLSVLLSPIGYVIQDTVADAMTVEAVPRVDERGVP
                     IDEARLKLMHTTMQTLGRVAIISGGILVALINLYLFNGVESMPKEQVASIYGQVYLLA
                     LVIPAVSVLGVITASILRVRARRRLLSQGMTLEQVATLTDRAAEPTNPNWWILGGSLA
                     FVLFTLTVGVADFAYSEEIVFAGSMAIVSLLMVRLTRELAPDARHVLVGTALVVFVFR
                     AVPGPGEGATWWMIDQLKFDQQFLSVLSLIGSALTLAGMFLFRRFMAERSIAYVVGFL
                     TLAAFFLGLPIVSMYYGLHEWTAALTGGLVDARFIALVNTALESPLGQISMIPMLAWI
                     ANSAPANLKATYFAVMASFTNLALSLGQLGTKYLNELFVVTREVRDPATNLLQTPDDY
                     SQLGILLIVQALLGLALPFAAILFARFSRYRSA"
     gene            complement(4699..5772)
                     /locus_tag="PK224_11660"
     CDS             complement(4699..5772)
                     /locus_tag="PK224_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRB16437.1"
                     /translation="MGKMSIKWMVGTVVVATVGAMGLAATRVMGEPSVAQPGAVPVAQ
                     EVPLGLEDPSGYIPADNPQTARKIELGRWLFFDKRLSKNGTVACATCHMPGLAFTDGQ
                     PVSTGIYRLQGGRSAPTAINRVYSKAQFWDGRADTLEDQSVGPFVSPVEHGFLDHNEL
                     VSKIKSIEGYRMLFREVFGREVTVGDVGRAIAAFQRTLLSGNSPVDRFDMGGDEQALT
                     ASAQRGLELFRGKARCTRCHSGFNFSDEKFHNLGIGWDTNTVDLGRYMVTKNAEDIGA
                     FKTPTLREIAGTAPYMHDGRFATLEDVVRFYNQGGIKNPHLDNTVIPLELTEPEQQDL
                     VAFLRSLNGEGWQQVAAPVEFPK"
     gene            complement(5820..6026)
                     /locus_tag="PK224_11665"
     CDS             complement(5820..6026)
                     /locus_tag="PK224_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16438.1"
                     /translation="MRQIVIAVGMGFAVIMLQACATPSRPTGHQTVAFSEGQQTPLSE
                     EEFLQKVERHMERQDRPGTTLRFR"
     gene            6159..7073
                     /locus_tag="PK224_11670"
     CDS             6159..7073
                     /locus_tag="PK224_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HRB16439.1"
                     /translation="MTLTELQYIVAVSRERHFGRAADRVFVTQPALSLAIKKLEDELG
                     VAIFERRKNHVELTPLGEQIVHQAQRVLEEADQIKLLAAQGKNQLVGALRFGVIATVG
                     PYLLPDLIPLLHKRAPGMPLEIEENLTVNLTAMLKSGKLDVTMIALPFEEPGILTQAL
                     YDEPFKAVVPADHRLAKRSKIDSALLSTERVLLPHAGHCFRQQVLESCPELSRSDTEI
                     LQGNSLETIRQMVASGLGITVMPCSAVTAKHQNKRLAVLEFMKPVPERRIALAWRKGF
                     TRPAVIPVIQEAVGALKIPGLMAIASGQ"
     gene            7099..7449
                     /gene="uraH"
                     /locus_tag="PK224_11675"
     CDS             7099..7449
                     /gene="uraH"
                     /locus_tag="PK224_11675"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRB16440.1"
                     /translation="MAESRISTHVLDIAQGRPAQGIPVTLEGQGLAGIWKLLSKSRTD
                     ADGRSNDLYPAKQRLQPGVYRLTFDVSTYFRSQHVTSFYPEVVVIFTLSDAGQPYHIP
                     LLLSPFGYSTYRGS"
     gene            complement(7477..8157)
                     /locus_tag="PK224_11680"
     CDS             complement(7477..8157)
                     /locus_tag="PK224_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRB16441.1"
                     /translation="MGWMCALSWTGLLFMASVSLAGAEPGHRTGDQALIAAAERNDVE
                     AVRQLLKEGASVRARDPQGRTALLAATYRNHVEIAKLLILTGSDVNTQDAMQNSPLLL
                     AGASGYLDILRMTLAADPDFTIYNRYGGTALIPACERGHVEVVKELLKTKIDVNHVNR
                     LGWTALLEAIILGDGGPTHQTIVGLLVAAGADLNLGDGDGVTPLRHARQKGYRVIADI
                     LLSAGARE"
     gene            complement(8169..9032)
                     /locus_tag="PK224_11685"
     CDS             complement(8169..9032)
                     /locus_tag="PK224_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB16442.1"
                     /translation="MADLRKRLDANRAGNFYVDSTCINCDACRQLAPSSFEEVGEYSA
                     VTHQPATEPERRQAYRALLACPTGSIGAEHTNQTLLSAAKADFPLPIEGNVYYCGFNS
                     EKSFGANSFFVQHPDGNWLIDSPRYLKPLVDVFDRMGGLAQIFLTHEDDVADAARYAR
                     RFGARRIIHRADVHAMPEAEVIIEGPEPRSYSEDFRIIPVPGHTAGSLCLLHRDRFLF
                     TGDHLWWDPEARELASPQQLVWNAEALHRSIEQLTNCSFEWVLAGHGGRVWLPQSEMR
                     AAVRELVERRQ"
     gene            complement(9025..9702)
                     /locus_tag="PK224_11690"
     CDS             complement(9025..9702)
                     /locus_tag="PK224_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRB16443.1"
                     /translation="MNQPSPEIAHLCKIDPVMKRVIGEVGPYALSLRARRSPFESLAR
                     AIAYQQLHDKAAESILKRFIALFPGLRFPRPADLLAMAPEIIRGTGFSHAKIAALHDL
                     AAKAMDGTVPTTTVIQRLEDDAIVERLIAVRGVGRWTVEMLLIFQLGRPDVLPVDDFG
                     VRNGFRIAYKRKAMPTPKELLVYGERWRPYRTAAAWYLWRAADRAKVVRTTLPVVRAA
                     RKRAPNG"
     gene            complement(9886..10269)
                     /locus_tag="PK224_11695"
     CDS             complement(9886..10269)
                     /locus_tag="PK224_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRB16444.1"
                     /translation="MARQNVSTGGPWEAKIGYSRAVRVGQSVQVSGTTAMTATGLVGK
                     SDPYAQTIQALKTIEAALVQAGASMADVVRTRIYMANIDQWQEVGRAHGEVFGSVRPA
                     TTMVEVKRLIDPDMLIEIEADAIVS"
     gene            complement(10449..11180)
                     /locus_tag="PK224_11700"
     CDS             complement(10449..11180)
                     /locus_tag="PK224_11700"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRB16445.1"
                     /translation="MKALRGKVAIVTGSSSGIGRAIAERLAQDGATVVVNYHVHEEKA
                     REVAAGIQASGGAAAVIQADMSRVADAQRLVQETVQQFHRLDILVNNAGRFIPKKLVE
                     TTETEFDAILALNAKGPYFAMQAAAKVLSDGGRIVNISSALTHLKFPGATAHLGSKAA
                     LEQYGKGLAQELAPRGITVNTVLPGFTDTGVLTEPYREMGLQSSPFKRLGLPSDVADV
                     VAFLVSEQARWLTGQVIQAGGGLVM"
     gene            complement(11369..12313)
                     /locus_tag="PK224_11705"
     CDS             complement(11369..12313)
                     /locus_tag="PK224_11705"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XamI family restriction endonuclease"
                     /protein_id="HRB16446.1"
                     /translation="MGWPHGAKYWSDVQIEKDAKVAREIFRQKRFQEPKELYLRAFEN
                     LEQANKKVVALLPKILADPVDPKLIASLIQEEHLLTAFRYLGAPPISEADLITLIDST
                     LAYTHIATSPERAGMIRDVIRPILDPKRFPWIYENRSPTPIEEEAAILASTVAASAQR
                     TQTGRRSEDRDAVEGAVRVILMNLRFRHIPSPRLGIQTLLGGHAPKPGHFMVGCKVGD
                     HNGDIVAGLYDGRILALECKASNSELNSRKRINKEVAVDAASWIRKFGEQNIVPAAAI
                     QGLFKPKYLIEAQETPVVFFWAHRLGDLEEFIKSTKGR"
     gene            complement(12323..13912)
                     /locus_tag="PK224_11710"
     CDS             complement(12323..13912)
                     /locus_tag="PK224_11710"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB16447.1"
                     /translation="MHRPPLIDPAARPEPILDYTALAKLADEFLSQEKVGKGSTHVQH
                     IRECILQHGDPLGETYTRLVSPDVRRQYGATFTPPAIVRAMLAWAKSQHRQFVRVIDP
                     GAGSGRFALAAARAFPKAEIIAVEKEPHIAHLLKATVIAAGLAHQIRVVVQDYRSLEL
                     PNVTGATLFVGNPPYVRHHAIEAQWKRWYTAALKAYDLSGSQLAGLHLHFLLKTRQLA
                     RPGDCGCFITAAEWLDVNYGSSFRTMFTNGMGGQAIHLIDKTVPVFDDALTSAVILCF
                     QVGAMENAMRMRHVTSIKELADLRGGRRISHSRARASDKWSQLGLGTVYEKANTIELG
                     DLFDVHRGQVTGMNAVWIAGEQAQQLPCQLRVPTVTAAKELINAPDHRLDSSAGLKSV
                     VSLPADLNELDASTFERVQDFLSWAKSQGAHRSYIAQHRRPWWRVDMPAPAPILMTYM
                     ARRPPAFVRNLCGARIVNIAHGLYPRVPLTDEDLDRVTDWLNANVRISSGRTYAGGLT
                     KFEPREVMRLRIPPLELMRQR"
     gene            14016..14354
                     /locus_tag="PK224_11715"
     CDS             14016..14354
                     /locus_tag="PK224_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16448.1"
                     /translation="MHDRESAEGDNLRYFPSLPFETDRFDLALSSHFLFLYSDHFDAA
                     FHLAAIRALLRVAREVRIFPLLALRAERSQHLEPVCEQLRQEGHHLSIERVGYELQRG
                     GNEMLRLRRA"
     gene            complement(14365..14970)
                     /locus_tag="PK224_11720"
     CDS             complement(14365..14970)
                     /locus_tag="PK224_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRB16449.1"
                     /translation="MEKPADVTHPIHALLQRRWSPRAFADRMVEPEQLQSLFEAARWA
                     PSSNNEQPWHFIVGTKADPSAHDRLVACLKEGNRKWAFRAPVLILSVARLNFEDEGTP
                     NRHAWHDTGMAALSLCLQATALGLVTHQMAGFEIEKARTDLGIPAGYEPVAMIAVGYP
                     GAPASLPDYLRERELKPRERKAVTEFVSHGRWNGPPEWPTR"
     gene            complement(14997..15560)
                     /locus_tag="PK224_11725"
     CDS             complement(14997..15560)
                     /locus_tag="PK224_11725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRB16450.1"
                     /translation="MAEKTEKNKMLGGELYRASDKELLDERRHAQQRLARYNGLPDSG
                     HDMRTTLLRHFLSAVGEGTVVLPTFTCDYGYNISLGRNVFINYHCIFLDCAPIAIGDD
                     VQIGPAVQLYTAQHPIDAKVRRSGLESASAIRIGNDVWIGGGALVLPGVTIGDRSIVG
                     AGSVVVHNVPPDSLVVGNPARIVRTLV"
     gene            complement(15582..16280)
                     /locus_tag="PK224_11730"
     CDS             complement(15582..16280)
                     /locus_tag="PK224_11730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRB16451.1"
                     /translation="MTVSQDPDSVAFLFDLDGTLVDSVYQHVLAWREATQAAGIELPV
                     WRIHRQIGMSGGLMLHALWRETGRPVSKDEAERIQRVHADAFAKQAPSLRVLPGAQEL
                     LNALTAAHVPYAIATSGRLQSAQHTLKLLKLPPGTPVVTRDQVKRAKPDPDLFLAAAE
                     QLHMPIARCIVVGDSVWDLLAARRASALGVGLLSGGYGREELERAGAYRTYDDPADLL
                     RHLDECGVRPEPPV"
     gene            complement(16286..16711)
                     /locus_tag="PK224_11735"
     CDS             complement(16286..16711)
                     /locus_tag="PK224_11735"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRB16452.1"
                     /translation="MKRNASAKWQGDLKTGKGTVSTDSGVLSETQYSFSTRFENGKGT
                     NPEELIAAAHAGCFTMALSGQLGAANLVAEQLATTATVTMEKVEAGWTVTGVHLSVTG
                     KVPKADAASWEKATAAAKAGCPISRLLNATITMDAKLEN"
     gene            complement(16813..19209)
                     /locus_tag="PK224_11740"
     CDS             complement(16813..19209)
                     /locus_tag="PK224_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HRB16453.1"
                     /translation="MWIPRDKSILSGPDQGEEEAKPAPPLLSANLPFAIDPSWVLVES
                     GFEPGWEHEIESIFTMANGYVGTRGSLAERTSLSSPATFLAGAFEASEAPGGTLELTK
                     LPDWTQLRVLVEGVPLSLETGTTLDHRRILDLQHGLFFRTWRHEDEQGRVTALTCVRA
                     VCHHDRHLLLQSVTITPENYSGTISIESFTEGPQPGTQWSSRVEGEAAVIVRRTDRGT
                     TIAMASHNKLRHENSQEGAGNSPVEGFAEQWTWQAQRGEAARFDRSVVVYSSREVEDP
                     AAAAGEHVRRVSEASLNAHMLQHAEAWEDRWRASGVEVAGDEMAQRALRFALYHLNSA
                     VNPKDEYASIGARALTGSTYKGHVFWDTEIYLLPFYTFTDPPAARALLQYRFHTLPAA
                     RDRARRLGYQGALFAWESADTGEDVTPDTVIAPDGRVVQVLTGIQEHHISADVAYAVW
                     QYWQATADEAFFLAAGADILIETARFWASRGRLEPDGQYHIRTVIGPDEYHESVDDNA
                     YTNVMAQWNLERAADTVDWLRSRRPDDWPIVCERLQVKPDEPDSWRRTAELMTTGFDR
                     PTNLFEQFSGFFQLEEVELQAYRTLPVPIDVTLGRDRTQKSKVVKQADVVALSALLWE
                     RFPSAVHEANFRYYEPRTAHGSSLSPALHALVSARLGDSDLAARYFRDAAAIDLSNHR
                     GNAAGGVHIATLGGLWQAAVFGMGGVRLREDGLVIDPHLPSNWDRLSFPLQWRGRSVS
                     VTIDHEPGQVTVDLRTGEPMTIELSHGPIQEITSNHLYVAHRTGSGWGAWQESRRRSG
                     "
     gene            19313..20089
                     /locus_tag="PK224_11745"
     CDS             19313..20089
                     /locus_tag="PK224_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HRB16454.1"
                     /translation="MTQGATGEQPFHPWSIDLTKFDAVLFDLDGVLTKTAVVHAAAWK
                     RLFDEYLQAKASREQVPFRPFDVMLDYQRYVDGKLRYDGVRAFLESRHIVLPNGTVHD
                     GPEQETVQGLGNKKDGYFQAHLKQHGIELYDDAVRFLRTLRAQGVRTAVVSASKHTAA
                     VLQKTGLADYFDTRVDGIESERLHLAGKPAPDGFLEAARRLQVDPPRAIVVEDAVAGV
                     QAGRNGGFGLVIGVDRTGHAEELTRNGAHLVTSDLAELCV"
     gene            complement(20230..20568)
                     /locus_tag="PK224_11750"
     CDS             complement(20230..20568)
                     /locus_tag="PK224_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16455.1"
                     /translation="MRHMKGFFATLFTAGLIFSAGPLFAEVDDGSHITITSPKDGAQV
                     GETFELTYELTKGSKAAHGHVYLDGEPQKKFPGTFKGLNKGKHEIKVQAATHDHDHLA
                     ATDTITVEVQ"
     gene            complement(20671..21000)
                     /locus_tag="PK224_11755"
     CDS             complement(20671..21000)
                     /locus_tag="PK224_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRB16456.1"
                     /translation="MTGKIAKHLDLDNQQKAKLAVVKDEVIAARAESQQEHRVIMEDV
                     IVQVQSDRLDQSKVAQLFERHQAGQTRLMQRVLPKVAEWHATLRPDQKAEAVEHLRKW
                     MERYGEH"
     gene            complement(21099..21395)
                     /locus_tag="PK224_11760"
     CDS             complement(21099..21395)
                     /locus_tag="PK224_11760"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRB16457.1"
                     /translation="MPVTLINVFSVPHTKEAEFVQWWQGVKEHITKQEGFISGKFHKS
                     IKPDSQFNYINVAIWENEEVYWKAYEKSVTPMKAKLGELGVEMTPALYRVAFEY"
     gene            complement(21524..22441)
                     /locus_tag="PK224_11765"
     CDS             complement(21524..22441)
                     /locus_tag="PK224_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16458.1"
                     /translation="MTRTMVIFGSMVLSLSMAVPYGFAAEGGGKKATHMKVSGVVSNV
                     QSGITTVKTPWGTMKIASTLTPKNLEVGEEVDMQVNENNAVIDVHRKGDKAHSHRFVT
                     GNLAYTSPDKKEIKVWTPEGEKAIDVQTGKSKLSALEEGAPVTIELNEAGKMIDIHRF
                     TVEMSFDEHPRTKPGYVIQVDGTVTKFDASLAYVKTPAGQYTLLKKYAPSDAAVGDHV
                     SLWINEEGMVIDVHGKDKAKAGTHRLIFGKLIRTGPNKNQIKLATPEGEKVFPLERME
                     IKTKPIADGDNIVVELNEEGTVIDLRKAQ"
     gene            complement(22455..22742)
                     /locus_tag="PK224_11770"
     CDS             complement(22455..22742)
                     /locus_tag="PK224_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRB16459.1"
                     /translation="MLKITVSNTNPGTTFVLSGRLAGPWVRELRDCWLKQEFDGPVPC
                     HVDLREVTFIDEAGTTLLSQMYRMGVTIQASGCLTRAIVQNLGKTGVHNHG"
     gene            complement(22804..23304)
                     /locus_tag="PK224_11775"
     CDS             complement(22804..23304)
                     /locus_tag="PK224_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRB16460.1"
                     /translation="MYSFFRTDESWAGLILRVVLGGVIFAHGAQKLLGWFGGNGFDGT
                     MGFFTNVMHLPWIVAFLVIIGESIGSLALIAGLLTRFTAASFIVIMLGAIMTVHLPQG
                     FFMNWFGQQQGEGFEFHLLVIAMSLVLLVVGGGKWSLDGVIARWLGEESPALKAREAS
                     FALRTF"
     gene            complement(23326..23457)
                     /locus_tag="PK224_11780"
     CDS             complement(23326..23457)
                     /locus_tag="PK224_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16461.1"
                     /translation="MKDMTLWGWWYGMLVTLPLFVAGLFLKDRMASAVPQRVRSHRR"
     gene            complement(23783..24826)
                     /locus_tag="PK224_11785"
     CDS             complement(23783..24826)
                     /locus_tag="PK224_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRB16462.1"
                     /translation="MHTLLVMVMVSLLMMVAPAQADTPGAWSKGAPFPEPSEELVGLS
                     TGGKFYVFGGLGPGWIPQGLVYEYDPSANKWTKNKPMALPAHHVAFTELNGKFYAFGG
                     FVPPQSGPPAWVPINNAWEYDPAHDSWKALAAMPSARGSAVAGMVNGKIYVIGGGAMH
                     PGSKEAALYPTRPHRSVGTVEEYDPKTNSWSERSSMPTARNHAAVGAVNNKIYVIGGR
                     LGSAFIFTASNTNVVEEYDPATDQWGLVRARMPTERSGGAWGVYNGRIYVAGGEHQDG
                     HLMAAFRALEAYDPATNSWSELPMMPMPRHGLAGAVVGHRLHLASGDIQSAGITGMHV
                     VTDAHDIFEFSDR"
     gene            complement(25015..26169)
                     /locus_tag="PK224_11790"
     CDS             complement(25015..26169)
                     /locus_tag="PK224_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB16463.1"
                     /translation="MTARQQAVTWGRRLAAMTWKEILQLSRDVPLLLFLLYSFSLSVV
                     VSGAGITMQLTNAELLVHDADHSHSSRELIHRFQPPYFKFAGEISDPREGLRQLDQGK
                     VMVLLEIPPRFHEALMSGERTAVQLLVDTTNAPQGLSAAGYSVRIAGLFGTETGLASA
                     GLSGAEGAIPRVSSAHRVWFNPTQDERWFQSIAHVLRMTTIFAVLLPAAALVREKERG
                     TVEQLLVSPLSPFQIMFSKVLAMSGVILIALSLALYGVLHPVFHVPMKGSTGLFFLLT
                     TLHVFTTAGFGLVAATMAKNQAQVGMMTLFVVGPMLLLSGLTSPYESMPQWVQTVMTF
                     SPLRYYIDITYGVMLKGAGLDLLWKPVVAMLLLGGALFGFGMWRFRRQFQ"
     gene            complement(26180..27310)
                     /locus_tag="PK224_11795"
     CDS             complement(26180..27310)
                     /locus_tag="PK224_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB16464.1"
                     /translation="MALKEWKETTRDRLFLLLAFLLPALWLVVFGYGLNLDVENIPFA
                     VLDRDRSELSRDYLHRFMQSRYFSFQGYADDERALDRLLTETKIRAAIIVPERFQEQL
                     AAGESASVQTLLDGTFPLHTDIAKGYVIAINQAFTEERLIEYLRRTRGLTQEQADALV
                     RPLTVEVRYLYNEEVRSTWSMVPALVMFTLMLASPLLTALGVVREKETGSIYNIYSST
                     VSRAEFLTGKLLPYIVISLVNVCVLWLMAVGLFQVPFKGSFLLFFSASVLFVCCTTGI
                     GLLISLLVQTQMAALIITMVVAMIPTILFSGLLVPVASLTRGAKVQAHLYPAMYYTNI
                     VRGSFLKGVGADVLWTDLLALAMFAVALSGLTYRLFTKRPKT"
     gene            complement(27331..28218)
                     /locus_tag="PK224_11800"
     CDS             complement(27331..28218)
                     /locus_tag="PK224_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB16465.1"
                     /translation="MALKEWKETTRDRLFLLLAFLLPALWLVVFGYGLNLDVENIPFA
                     VLDRDRSELSRDYLHRFMQSRYFSFQGYADDERALDRLLTETKIRAAIIVPERFQEQL
                     AAGESASVQTLLDGTFPLHTDIAKGYVIAINQAFTEERLIEYLRRTRGLTQEQADALV
                     RPLTVEVRYLYNEEVRSTWSMVPALVMFTLMLASPLLTALGVVRDIGQLVEMVVDKPG
                     IALAHVVAAGFAGAALFGTKIHVLSRDPGRDEERLRDVLARSGIAVEAVRTRMLSLED
                     VFVSRVMALEQAAQKEVSA"
     gene            complement(28239..30218)
                     /locus_tag="PK224_11805"
     CDS             complement(28239..30218)
                     /locus_tag="PK224_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB16466.1"
                     /translation="MTKPAASSPEVVVRVSGFVKSYKQHLAVDRLDLTVKRGEIYGLI
                     GPDGAGKSSVMKAIAGVLRYDGGSAEVFGTLVDSERAAERVKPRIGFLPQGLGLNLYP
                     ELSVEENIDFFARLRLVPEPELRARKSRLLAITRLDRFRSRLMKQLSGGMKQKLGLIC
                     TLIHEPELAILDEPTTGVDPVSRRDFWAILAEWQADKGMTALVSTAYMDEAARFHRLA
                     FLSHGRVLASGTPAEVQSLVPGLVVTFESSPQLEAVARLKRRYAQVESMGPLLHVFTP
                     EREPEAAVAGIRQALENLPVEHIHTEEPELEDVVVALLLKKREEQPGVSAGSDYSARP
                     AGRQQLDGLAVEAKELIRDFGSFRAVDRVSFDMKQGEIFGLLGANGAGKTTVIKMLTG
                     IVPPTAGEGRVAGADMRTAGGAIKERIGYMSQAFSLYLDLTVIENIRLFAGIYGLARR
                     EAQQRMEWIVEMAGLTGYEDDRTGRLPMGVRQRLALGCALVHSPRVLFLDEPTSGVDP
                     IGRRRFWELLSRLAREEGVAILITTHYLSEAEHCDRLALMYAGRIVAEGTPAHLKKQV
                     ERDIGQLVEMVVDKPGIALAHVVAAGFAGAALFGTKIHVLSRDPGRDEERLRDVLARS
                     GIAVEAVRTRMLSLEDVFVSRVMALEQAAQKEVSA"
     gene            complement(30215..31318)
                     /locus_tag="PK224_11810"
     CDS             complement(30215..31318)
                     /locus_tag="PK224_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB16467.1"
                     /translation="MTLRTSTIAAAVLASVLIAGYVILDRLVWQDGLPEGLIQANGRI
                     EGDHVTVSSKFPGRIVDLAAREGSQVHKGHVLIRLDDAQTRAKVDQAARLVDSMMAQV
                     EAAHTALAVLNLEVPLSIEAVAAKVASMRAVIRKAQAVEYEARRDTDRIRALLPEQAV
                     SQQQVDQAEARWKVAVTEIAVAKAALDQASKELAQAELGWGRIRSKEAEVAALERQRD
                     QADATFAEARSILDDLTIVAPTNGTVTTRMVDVGEVVATGAPLLELVDLDRLYLQVYV
                     PELQIGKVRLDLPARIHTDAFSDRSFEATVRYIASKAEFTPKEVQTPDERVKLIYAVR
                     LYLTENPDHRLTPGLPADAVIRWKDDVAWSKPR"
     gene            complement(31322..31762)
                     /locus_tag="PK224_11815"
     CDS             complement(31322..31762)
                     /locus_tag="PK224_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRB16468.1"
                     /translation="MKMQSTVRVAIGTLVIAGALVGAGCHRHHTPTERADWMTGKIAK
                     HLDLDEQQKAKLAAVKDEAMAVRAESEKERKATMEEVIAQVQSDRLDQAKLARLFDQH
                     QAGQTRLMQRVLPKLAEWHATLRPEQKAEAVEHLRKWMDRYGDH"
     gene            complement(31856..33946)
                     /locus_tag="PK224_11820"
     CDS             complement(31856..33946)
                     /locus_tag="PK224_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB16469.1"
                     /translation="MNDRPSLPTPQAMQGNSERYGALLQVVEAIASHADLQGLVQDLA
                     RLLPLLVPVNFVGLSLYDAQRDMMRLHVLRANVPADIIGGQESHPADTPAGLVWQTQQ
                     PVILSDLAEEHRWPKLIALMREDGVQSCCLVPLTSAVRQLGALAFSSLKKDAYRVSDL
                     ALMQQIGRQVAVAVENVLNREAAAAARQDVERQRDRFSLLLRMTNTMVSKLDLREVFS
                     AVSLCLREMVPQEYASLILCDGKNGRVRLHALDFPENQGALTEGAVSDVAGSLAGLAL
                     ERRRPAVANNRPDLTAFSHAVPQLLVMKGFHSMCSLPLLSQDRVIGCLNLASRQEQAF
                     GEQDVEFLSQVAGQIALAVDNALAYQEITELKDRLTEEKLYLEEEIRLEHKFDDIIGD
                     SRALKQVLGQVEIVAPTQATVLIQGETGTGKELIARAIHRLSGRSQRTFVKLNCAAIP
                     TGLLESELFGHERGAFTGAIAQKVGRFELAHGGTIFLDEVGEIPLELQSKLLRVLQEQ
                     EFERLGSTRTIHVDIRLIAATNRDLAKMVEDHEFRSDLYYRLNVFPITLPPLRERRED
                     IPVLVRYFTQHYAARMKKHIETIPGPSLEALSRYHWPGNIRELENLVERAVILTQGTH
                     LQVPLLELKIEARQAPAPTPTLQDAEREQVLRVLRDTHWVIGGPDGAAARLGLKRTTL
                     TSKIKKLGLSRPRE"
     gene            complement(34101..34625)
                     /locus_tag="PK224_11825"
     CDS             complement(34101..34625)
                     /locus_tag="PK224_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16470.1"
                     /translation="MNRQTFRCLALATVLAALSIASPVAHAAIGAEHLARGHTEFTSV
                     VTKKAGALVVTTPNGAIHQLNENMARRHGQEPFKAGDDVIVVLDENNYIVDMHLKGKE
                     GAHRQVTGKLIHVGRMKKQIKLQTPAGEQVFPLAEEGQKTKGMEEGELVTVELNEAGT
                     VIDMHRADIGGGGH"
     gene            complement(34664..35794)
                     /locus_tag="PK224_11830"
     CDS             complement(34664..35794)
                     /locus_tag="PK224_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRB16471.1"
                     /translation="MTNIPRIQTREITAAVVRKKGGPFQVETLTLEEPRPDEALIRIV
                     ATGMCHTDMVARDQLYTVPLPVVLGHEGAGIVERVGSSVKKIAPGDHVVLTYMWCGHC
                     KPCLNGDLTYCENFYPLNFGGAREDGSTATQDKQGAVHDHFFGQSSFGTYALAHERNV
                     VKVPAAAPLELLGPLGCGIQTGAGAVMNALKVRPGSSFAAFGGGAVGLTAVMAARVAG
                     ATTIIAADVVPSRLALAKELGATHTVNSRETDPVEAVRKITGGGADFTLESSGRPAVL
                     RQAIDALAIKGVCGIVGAPALGTEASFDVNGVMTTGKRIIGIIEGDSVPDLFIPSLVE
                     LYQQGRFPFDKLVKFYPLDQINQAAEDSEKGITIKPIVRLGS"
     gene            complement(35853..37394)
                     /locus_tag="PK224_11835"
     CDS             complement(35853..37394)
                     /locus_tag="PK224_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRB16472.1"
                     /translation="MASKFIALPKSVSEKSFAAAIAEFRRIVGQDSVLVTAEQLAPYI
                     KTMMPVPDADHTPSAALLATTVEQIQKIVGICNTYKVPIWMISTGRNFGYGSAAPAER
                     GQVVLDLKRMNRILEVDQDLCFALVEPGITYKQLYDYIQEHKYPLWLSCPAPSAIAGP
                     TGNTLDRGVGYTPYGEHFLFACGMEVVLANGEVLRTGMGSLPKSNTWQVFKWGYGPYL
                     DGIFTQSNYGIVTKFGFWLMPAPPAFKPFVIQYENEDDIVEIVETIRPLRISGVIPNA
                     IVIAHALYEAPTKAKRSDYVTGPGAITDEAVRQIMKDHKLGVWNVYAALYGTQEQIDV
                     NWKIVTDAFSKSGKAKILTEEEAGDDPAFQYRAALMRGDMTLKEFSLYNWRGGGSMWF
                     APVSQARGSETLKQMAMTKRILGKYGLDYSGEFIVGMRDMHHIVDVLYDKTDPAMTKA
                     AYQCFDELLTEFSNEGYGTYRVNTAFMDKVAHTYGPVQRHVHKTLKKALDPNGILAPG
                     KSGIR"
     gene            complement(37413..38291)
                     /locus_tag="PK224_11840"
     CDS             complement(37413..38291)
                     /locus_tag="PK224_11840"
                     /inference="COORDINATES: protein motif:HMM:NF021997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRB16473.1"
                     /translation="MDIDRRNLMKGLLAGGALLALGTPAWTFAEEPNRTAKRCLLFLG
                     DNYVDERFANGVRAACQEVGYDGLETRQVDGGLLSDPGKLVSLLEQTKGARWVAVMDD
                     ASAAVFQELARTAGGRLLSVGSHASAKDDACPLRHTWLAASSAQGAGGVLATRLIGAG
                     ESFSITESFLDGSSAASKLTNWSASGFSSYRSAGSDAIHLHCSGLSLLEGCAQLGLAG
                     VEGWTPIPSHVSQREVVSRQSPQWVESVGYAVAASALGIHSIPESCSSRAFVHRAFNP
                     HSLPPTQRFVSFVMDI"
     gene            complement(38306..38647)
                     /locus_tag="PK224_11845"
     CDS             complement(38306..38647)
                     /locus_tag="PK224_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB16474.1"
                     /translation="MQALKAVWTVPALILTAFVLLGNVVRIAGATSQETPESGFRWKD
                     GAEVCAKVCAFCHEAKVGPTIRGRGLAPAYITFIVRHGNRAMPSFRAAEIDDESLAKV
                     AEYVSNAAANQ"
     gene            complement(38957..39697)
                     /locus_tag="PK224_11850"
     CDS             complement(38957..39697)
                     /locus_tag="PK224_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB16475.1"
                     /translation="MGEAASENGYSITLTERRLVAEGTMAFYFDKPTQFAFTPGQFVD
                     LTLPQPSETDAAGNTRAFSIASAPQESTLMVATRLRDTAFKRELQRMPLGSTVRMEGP
                     FGKLVLHADQTRPAVFLAGGIGITPFRSMAVQAAMQRSPHPMVLFYSNRRPEDAPFLD
                     ELQALQDKNPHYRFVGTMTEPTKSSRPWQGETGYLNAALLSKHLVNMEKPIYYVVGPP
                     GMVGALRTMLKGAGIDDSDIRTEKFAGY"
     gene            complement(39743..39979)
                     /locus_tag="PK224_11855"
     CDS             complement(39743..39979)
                     /locus_tag="PK224_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRB16476.1"
                     /translation="MEEPVIAAKQPAVLELEAGTYYWCRCGRSKKQPFCDGSHKGTSF
                     TPMEFTTTEKKTVALCQCKHTKNPPFCDGTHKSL"
     gene            complement(40426..40872)
                     /locus_tag="PK224_11860"
     CDS             complement(40426..40872)
                     /locus_tag="PK224_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB16477.1"
                     /translation="MKAHYLGHVVFYVKDLGRSLTFYRDLLGFQEVGRIFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPSGRRRGLYHIGIKVGDSLDELRAAKRELESAGVSIDGM
                     SDHTVSQSLYLRDPDGNEVELYVDADEALWKNNPSAVLAPIKPLSL"
     gene            complement(40869..41291)
                     /locus_tag="PK224_11865"
     CDS             complement(40869..41291)
                     /locus_tag="PK224_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16478.1"
                     /translation="MAGEDHMRAHLGNLSRSLLRLHKALLDSERVSYERVHGRIETNG
                     AFFQLVLGDAWFAWLRPLSQLMVKIDELSEEEEIGDRTEMTLAIDSVRTMLTPSEEGN
                     GFGRHYYDALQREPDVVLAHAAVRALLRTSSPGKESIT"
     gene            complement(41321..42427)
                     /locus_tag="PK224_11870"
     CDS             complement(41321..42427)
                     /locus_tag="PK224_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRB16479.1"
                     /translation="MPDATWTEIGSVDELKQRPLQQIVCGQTTLALSYKDGRFAAISG
                     VCNHIGGPLGEGRLDGDYVVCPWHYWKFHHRTGRGEPGYEGDQVATYAVKVEDGRVFV
                     DLTPVTKRQKLPKPSHPLARPIVRADGPIRVLGIATTAMTADQPRFSASDALLEEALA
                     YAREHLQLDTQLIKLRDLSFRACEGFYSKSAEACTWPCSITQMDPTDQMDRVYEAVVH
                     WADVILVSTPIRWGGASSLYYKMVERMNCIQNQETIANRHLLKNKVAAFIIMGGQDNV
                     QGVAGQLMTFFAEVGCQFPQFPFIAHSRGWSAEDMERNNSEVQNSRELREGAQSLVAR
                     AAEMARLMVEGQLAMHPLTRGGRKAHQLDSEPTG"
     gene            complement(42465..42923)
                     /locus_tag="PK224_11875"
     CDS             complement(42465..42923)
                     /locus_tag="PK224_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRB16480.1"
                     /translation="MPDLEKVRTLAKDLTKTYPRSPREMLGGYVIGARCADKCRAFLL
                     GINGDYNYWPCSLAGLLFSFTGITPEQFKEVVATGANDEELAAWLKAQSKVQDQQAII
                     QWNNKMRDLRLSEMAPQVQAYMEDYIPKYVPSHRPVYVYFDVYDLEEKRL"
     gene            complement(42928..43365)
                     /locus_tag="PK224_11880"
     CDS             complement(42928..43365)
                     /locus_tag="PK224_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB16481.1"
                     /translation="MPVQVYGCNHIVIEVTDAKKAVKFYSDVFGLKMLRGGEGAAWCK
                     LGEHQFMAIFEVEQLQPDRVKHFGLMVRDAEQIKEVRRKLTRKYKLRMEPHFRCDFRD
                     PWGNRIQVGDLSDESLVWLLPYQEVQKAGITFSKPSRVRQGRE"
     gene            complement(43378..44019)
                     /locus_tag="PK224_11885"
     CDS             complement(43378..44019)
                     /locus_tag="PK224_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRB16482.1"
                     /translation="MTGQALTVDVYSDVVCPWCYIGKRRLEQALESVQVQATARVFWR
                     PFQLNPTMPKAGMDRRVYLEAKFGGPGEMKAIQDRVAAVGTSVGIEFVFDRIARTPNT
                     FDAHRLIWFAQQQGRQDEVVEELFHGYFTEGLNIGQPEILVSLAVRAGLDGEAVVRLL
                     HAQEGVDAVRQEEAHGHQLGIRGVPYFVLNGKAALSGAQPVETFVSAIIQVTG"
     gene            complement(44016..44900)
                     /locus_tag="PK224_11890"
     CDS             complement(44016..44900)
                     /locus_tag="PK224_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRB16483.1"
                     /translation="MRTDTSVTKDLVGVYQAGSHHMVGDGFPVRNMIPGAGVEEQLSP
                     FLMLDYLGPQQFPPTDRQLGVGEHPHRGFETVTIMYHGKVAHRDSTGSGGVIGPGDVQ
                     WMTAASGVVHEELHEKEFAKQGGLLEGIQLWVNLPKAFKMTAPRYQTLVDAEIPAVEL
                     DGGVGRVRVIAGEFRAVKGPAKTFSPVHLYDLRLKAGHRTELNLPEGYNSSLFVLHGE
                     VVMNGSQTVQEVEIALFGQRGERVVVEAKQDATILVLSGEPIAEPIARYGPFVMNTRD
                     EIIQAVHDYQAGKMGHLS"
     gene            complement(44914..45771)
                     /locus_tag="PK224_11895"
     CDS             complement(44914..45771)
                     /locus_tag="PK224_11895"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HRB16484.1"
                     /translation="MFVVLGATGHTGAVVAETLLARKQSVRVVVRSADKGAAWKSQGA
                     EVAVASLDDVPALTKALQGALGVYLLVPPNYGATAWFAEQRQRLDRAAEAVKASGISH
                     VVFLSSVGGQIPEGTGPIRAVRYGEQKLSPVARNLTILRPCYFMENWAPGIGMAKGQG
                     LLPTFMAPQAKVPMIATRDIGRVAAERLMAGGRGHTVVELAGPEEYSPEQVAEALGRI
                     LGRAVATQPAPLSAVVPTLTSFGFSREAAGLFEEMYTAFSRGAIGYEHPASLVRGTIP
                     LAEALRGMA"
     gene            complement(45793..46239)
                     /locus_tag="PK224_11900"
     CDS             complement(45793..46239)
                     /locus_tag="PK224_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRB16485.1"
                     /translation="MKALFNTDDGWSGLILRLTLGLVMLPHGAQKLLGWFGGFGFDGT
                     MGFFTQKMGLPWIIAFLVIMGEFFGSLGLLAGLLTRFTAASFIVIMLGAILTVHLPVG
                     FFMNWFGQQQGEGYEYHLLVIGLAVALLVTGAGRWSIDRIVAERVA"
     gene            complement(46566..47045)
                     /gene="smpB"
                     /locus_tag="PK224_11905"
     CDS             complement(46566..47045)
                     /gene="smpB"
                     /locus_tag="PK224_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRB16486.1"
                     /translation="MTKTHDKEDQYKVVATNRKAYHDYFIEEKFEAGVILRGTEVKSL
                     REGRVNLQDSYASVDDGEVYLQHCHISPYSHGNLMNHDPLRKRKLLLHRKEINKLIGK
                     TQLKGLTLVPLRIYFSKRGHAKVELALAKGKKQYDRRESIKAREAGREVERAIKQRK"
BASE COUNT         9533 a        15065 c        13063 g         9487 t
ORIGIN      
        1 ggaccgtctt gaactcgcga tctagaaact tattcgaaca gatgccatac gtatccgatc
       61 cggcatccgc cgaaatctga aacgagacgg cgcccggctc cacagttccg cccgcgagca
      121 ccgtgacacc gcgcggcacg atgaaacagc gcagcacctg cttcgccgcc gcatcccaca
      181 gccagtatcc ggtttcttca tgaaacggcg ccccatcgct caaccgccag gccacggtcg
      241 cataacgaag tccgtaaagc tgttgttcat gattgttgac cggaccgaac ggctcgaagg
      301 tcaaccgctc gcgataggca ttctgttccg tgccccggtt gtcatcaggc gccacatcgg
      361 cgcccttatc gccttcccac acaccggcca gcgctgccaa cggaccgaga tacttgatca
      421 gatcgtcacc catgtcatcg ttcctttcat gcggttgcct gttccatcgg tgtgcgaatc
      481 tacattcatc ggcccgcaac cggcaacaat gaaaaagccc ctgagtcgta cgactcaggg
      541 gcttagtttg gtggaggcga ggggagttga acccctgtcc gaagatcgtc ggcacatcgc
      601 gtctacatgt gtagccgatt ctttaagctt cgcagcgacc cacgcctatc ggccggctta
      661 gaatcgccgc tagcccagta ttgtctcgcc ccgcgccgct gagcaccggc gtgggaccag
      721 tccgctgcat cgcgccattc cacccccgcg gacctcagat ggaacgacgt ctcagcctaa
      781 attaggctgc gagtgccagt tcttggttgg cagttgcgtt tttcccggaa ttttacgagt
      841 tcccgagatc tcgacatgcg acgatggcgt caatatcccc gtcgaaaccg gtcgccccct
      901 ttcttggtga tcgtgaaacg tcagacgcaa cacgtgagag gccattcgct catgaaagga
      961 ttcggcgact tctccgcttc ctttcgcgtt tcactgttca cgtctcaata cgtcttagat
     1021 gctcatcata cagcacaggt caaggggatg ccaggagaga aacggtacgg tgggaaacca
     1081 gggcagggaa actcacggaa ccggcgtgaa ggtccaggtc catccctgat cctgttgcgt
     1141 gaagacctgt cccttagaca actggcccgc gaggaactcc ggaggaagtg tcaccagcac
     1201 ctgttcctcg cgcacctgcc cctgaaccag gcgtgtgccc ttgcagccgc cacccccgac
     1261 atattcctcc atatccggcg ttctccgttc acccaccagc ccgaacatcg gtcccatcac
     1321 cagaatgctc ttcaactcaa aatgctcata ggcccctttc agaatcggtg ccgggcagac
     1381 cgccgagcca tcacgatagt gctccgcctt cgtcggaata tccggcagca cttttggcga
     1441 caccccatcc tccagcggcc acttgaattc gaactcgacc tgcttcgtca cgtcggcgac
     1501 ggccgaacca ccgacgaact gcttgttcct ttgaaagact cccaccactt tgtacttcaa
     1561 cggtgcgggc ttcgaacggg aggacccttt cgtgagccag accatggctt cgctcacagt
     1621 ggtcgacagc tccggccatg cgcctgcctc acggccgctc gacatgagca cggtccctgc
     1681 gatcatcagc accaccagac gtgtcacgcg ttcactcctc tgcaagctag cccggactat
     1741 tcatgaatac ctgctccgtc gtccgattct ctcacccggc gtggctgttc tcattcggcc
     1801 tccacgacgg actgccagta cgcctgcgtc agcctcacgt gcgagaagcc gcggcgggct
     1861 tcggtctcga acgcctcgcg acggcattca tcaataatcc gggctagaga caccccctag
     1921 cgaccttccc gcatcccgcg ccgcggtacg ccgtcagccg cctacaccgg cgttcacccg
     1981 tcgctccacg ggatactgcc gttctcgcca ggcatccaga ccgccttcga gcgggcgcac
     2041 acgatggatg cctttcttct tcaacagaaa cgccgtcctg gcgctggaga cttcattcgg
     2101 acaggtacaa tacagcacga tgtcgcgatc gcgagggatc tcctggtgcc gatgctcgat
     2161 ctcttccaac gtgaagttga tcgcaccggg gatggtgtcc ggatcgagtt gcagcgatag
     2221 gggatgccgc acatccacca cgctgatcgc ctcgcccgca tccagccgct gtttgagctc
     2281 gtccacggag atcttcgcca tccgcagatg cctgaggaac tgctgccggt aatagaactt
     2341 atagccgatg aatcccgcca ggccgatgag cagcaccccg aacaccagtc cgcccgcttg
     2401 atccaacaac ccgacgagtt gctccagttg attgctgaat aacgcgccga cgccggcgct
     2461 gaccgccgcc cagatcaacg tgcccgcgac atcgtacagt gcgaatgtcg tcgcggccat
     2521 gccgacaatc ccggccagcg gcggcgccac cgtattgaaa ccggggatga acttggccag
     2581 caacagacca cgtggcccgt accggtgaaa cagattttcc gtatccctga cgcaggagtc
     2641 gggctcaagc gacaggcgac agaggaaacc cagcaccttg ccgcccttga tgcgaccaag
     2701 gtagtaccag gcgaggtccg gcgggagcgc agcggccacc ggcaccagca gcgccgtcgc
     2761 aaggctcatt ttcccggctc cgacgagcgc gcccgccgca atgaggagag gaatcgccgg
     2821 aatcgggaat ccgatctgct caacgaagat cacccagaac agcaccgagg cgccgtgatc
     2881 ggcgagaaat tggaaggtgt tcatggcgat ctccttccgt gcgctcaggc gctgcgatac
     2941 cgtgagaatc gcgcaaagag aatggcggcg aaggggaggg ccaggccgag cagggcctgc
     3001 acaatcaaca agattcccag ctgactatag tcgtcaggcg tctgcagcag gttggtggcc
     3061 ggatcgcgca cctcccgggt caccacaaac agctcgttga gatacttcgt ccccagttgg
     3121 ccgagcgaca gcgcaaggtt cgtgaaggac gccatcacgg caaaataggt cgccttgaga
     3181 ttcgccggcg cagagttcgc gatccaggcc agcataggga tcatggaaat ctggccgagc
     3241 ggcgattcca acgccgtgtt cacgagggca atgaaccgcg catcgaccag gcctcctgtc
     3301 aaggccgccg tccactcatg aagcccgtaa tacatactga cgatgggcaa acccaggaag
     3361 aatgccgcca gggtgagaaa gcccaccaca taggcaatgg accgctcagc catgaatcgc
     3421 cggaacagaa acatgcccgc gagcgtgaga gcgctgccga tgagcgacag caccgacagg
     3481 aactgttgat cgaacttcaa ctgatcgatc atccaccagg tcgccccttc gccgggccct
     3541 ggaacggcgc gaaacacgaa aaccaccagc gcggtaccca cgagcacatg cctcgcgtcg
     3601 ggagccagct ccctggtgag gcgcaccatc aacaacgaga cgatggccat cgacccggcg
     3661 aacacgattt cttcactgta ggcaaagtct gcgactccga ccgtcagggt aaagagcacg
     3721 aaggccagac tgccacccag aatccaccaa ttgggattgg tcggttcagc ggcccggtcc
     3781 gtgagcgtcg caacctgttc gagcgtcatg ccctggctga gaagccgccg cctggctcgc
     3841 accctcagga tcgacgcagt gatcaccccg agcaccgata cggccggaat caccaacgcc
     3901 agcagataca cctgtccata gatcgaggcc acctgctcct tcggcatact ttccacgccg
     3961 ttgaacaggt acagattgat gagggcaacc aggatacccc cgctgataat ggcgacacgc
     4021 ccgagcgtct gcatcgtggt gtgcatgagt ttcagcctgg cttcatcgat gggcacccct
     4081 cgctcgtcga ctctgggcac ggcctcgacc gtcatcgcgt ccgccaccgt gtcctgaatc
     4141 acatagccga tcggcgacaa caacacgctg agcatgtacc agacttcggc cggcatcacg
     4201 gcgaccatcg cctcacggtg accgatcagt ccgaccatga tcaacagact ggccgcaatc
     4261 accccggcgc caaaataaac aaggagactt ttccagcgcc acaacagatc gaccagatgt
     4321 ccgaacggca tcttgagcgc ccagggaatg cccgcccaga atcccaacgc ggcaagaaag
     4381 gccgcggaca ggcccagata atctttgacg aagaaggtgc cgacgatgcc ggtcagaccg
     4441 gaaatcccgg cggccatata aaccatgagc ggcggcagat aggagagctg gaagtcgcgc
     4501 gcgagttcca gaatattgcg gtcgatccag cgggaggcag cagcaatcac gaatgcgtat
     4561 ccagggacgg cagtgaactg atcgttatct ccctctctgt tacccgtacg ggaccaccat
     4621 ttgcaagcaa cccggagagc cggcgggaaa ggggcggggc ctcctgaccc cgccccttcc
     4681 actcggcgaa cgtacggctt acttcggaaa ctctacgggc gcagccacct gttgccaccc
     4741 ttcgccgtta agcgaccgta agaacgcgac cagatcctgc tgctccggtt cggtcagttc
     4801 gagcgggatc acggtgttgt cgagatgcgg attcttgatg ccgccctggt tgtagaaccg
     4861 gacgacatct tcgagcgtcg caaaccggcc gtcgtgcatg tacggcgccg tacctgcgat
     4921 ctctcgcaac gtgggcgttt taaacgcgcc gatatcctcg gcattcttcg tcaccatgta
     4981 gcgccccaaa tcgacggtgt tcgtatccca gccgataccg aggttatgaa atttctcgtc
     5041 ggagaagttg aacccggagt ggcatcgtgt gcagcgggct ttaccacgaa acaattcgag
     5101 cccgcgctgg gccgaggcag tcaacgcctg ctcatcgccg cccatatcga accggtccac
     5161 cgggctgttg cccgacaaca gggtccgctg aaaagcagcg atcgcgcggc ccacatcgcc
     5221 gacggtcacc tcgcggccga acacctcccg aaagagcatc cgatagccct cgatgctctt
     5281 gatcttgctc accaactcat tgtggtcgag aaacccgtgc tcaaccgggc tgacgaacgg
     5341 accgaccgat tgatcctcca acgtgtcggc tcgaccgtcc cagaattgcg ccttgctata
     5401 cacccggttg atcgccgtcg gggcgctgcg tccgccctgt aaccgataaa tacccgtcga
     5461 gaccggttgc ccatcggtga atgccaggcc cggcatgtga caggttgcgc acgccactgt
     5521 cccgttcttc gagagtcgct tgtcgaaaaa gagccagcgc cccagctcga tcttccgcgc
     5581 cgtctgcgga ttgtcggccg gaatatatcc gctgggatcc tccaatccca gcggcacctc
     5641 ctgtgccacg ggaacggcgc ctggttgcgc caccgacggc tcacccatga ccctggtggc
     5701 cgccaagccc atcgccccga cggtcgccac caccactgta ccgaccatcc acttgatgct
     5761 catcttgccc atgatctcct cccttgctcc ttcttccttc gatgctcctt ccgtatggat
     5821 catcgaaacc gcagagtcgt gccaggccga tcctggcgct ccatgtgccg ttcgaccttt
     5881 tgaaggaatt cttcttccga caggggcgtt tgctgccctt ccgagaaggc caccgtctga
     5941 tgacccgttg gcctcgacgg cgtcgcgcac gcctgcaaca tgatcacggc gaaccccata
     6001 ccgacggcta tcacaatctg tcgcataccc cacctccttg ttccatggct gcattgttgc
     6061 gaggtcatac agagacgata ccggttgcct gagaatcagt ccaattgata gtctatggca
     6121 tcacgataaa ctgagcttat cgatagagga ggggagtcat gaccctgacg gaactgcagt
     6181 acatcgtggc ggtttcgcgc gagcgtcatt tcggacgggc ggcggatcgg gtcttcgtca
     6241 cccaaccggc cctcagcctg gccattaaaa aactggaaga tgaattgggg gtggcgattt
     6301 tcgagcgacg gaagaatcac gtcgagctca cgccgctcgg ggagcagatc gtccatcagg
     6361 ctcaacgcgt gctggaggag gcggatcaga tcaaactgct cgccgcgcag gggaagaacc
     6421 aactcgtcgg cgccttgcgg ttcggggtga ttgcgacggt ggggccgtat ctcttgcccg
     6481 acctgatccc gctgctgcac aagcgcgccc ccggcatgcc gctcgaaatc gaggaaaatc
     6541 tgaccgtcaa tctgaccgcc atgctgaaga gcggcaaact ggacgtgact atgatcgcgc
     6601 taccgttcga agaaccgggc attctcaccc aagctctcta tgacgaaccg ttcaaggcag
     6661 tcgtgcccgc cgatcaccgg ctggccaaac ggtcgaaaat cgattcggca ctcttgagca
     6721 cggagcgcgt gctcttaccg cacgcaggac attgtttccg gcaacaggtc ctggagagct
     6781 gcccggaact cagccggtcg gacacggaaa ttttgcaggg caattcgctg gagacgatcc
     6841 gtcagatggt ggcctccgga ctgggcatca ccgtcatgcc ctgcagtgcc gtcacggcca
     6901 aacatcagaa caaacggctg gctgtcctgg agttcatgaa gccggtgccg gaacgcagaa
     6961 ttgcgttggc atggaggaag ggctttacga gaccggcggt gattcctgtg attcaggagg
     7021 cggtcggtgc attgaaaatt cccggactga tggccattgc ctcaggacaa taattgtccc
     7081 acgatgaaag gaagaagaat ggccgagtca cgaatcagca cgcatgtgct ggacattgcg
     7141 caaggccgac ctgcacaggg cattcccgtc acgttggaag gccaggggtt ggcgggaatc
     7201 tggaaactac tcagcaagag cagaacggat gcggacggcc ggagcaacga tctctatccg
     7261 gcaaagcagc gacttcagcc cggagtctac cgtctcacat ttgacgtctc gacctatttt
     7321 cgctcacagc acgtgacgag cttttaccct gaggtggtcg tcatcttcac tctgtccgac
     7381 gcgggacagc cgtaccacat tccgctgctc ctgagcccct tcggctacag cacctatcga
     7441 gggagctgag agaagatcga cggtccactc tgccggttat tcccgcgccc ccgccgacaa
     7501 gagaatgtcg gcgatcactc tgtagccttt ctgcctggca tgccggagcg gcgtcacccc
     7561 gtctccgtcg ccaaggttga gatcggcccc tgcagccaca agcaatccga cgatggtctg
     7621 atgtgtcggc cctccgtcac cgagaatgat ggcttccaac aaagccgtcc agcccagccg
     7681 gttcacgtgg ttcacgtcga tcttggtctt cagcagttct ttcacgactt ccacgtggcc
     7741 tcgttcgcac gccgggatca acgcggtgcc tccgtagcga ttgtagatcg taaagtccgg
     7801 atcggcagcc aacgtcattc gcagaatgtc gagatacccg ctggccccgg ccaacagcag
     7861 aggactgttt tgcatggcat cctgcgtgtt cacatcggag ccggtcagga tcagtagctt
     7921 cgcgatctcg acatggttcc gataagttgc agccagcaag gcggtgcgcc cctgcggatc
     7981 acgggcccgg acgctggctc cttctttcaa cagctgtcgc acagcctcga catcgttgcg
     8041 ttctgccgcc gcaatcagag cctggtctcc cgtccgatga cccggctcgg cgccggccag
     8101 gctgacgctc gccatgaaga gcagaccggt ccatgacaga gcgcacatcc aacccatctg
     8161 attccgcctc attgtcgtcg ctcgaccagc tcgcgcaccg ccgcccgcat ttcggattgt
     8221 ggcagccaga cgcggccgcc atgaccggcc aacacccatt cgaatgaaca gttcgtcagt
     8281 tgctcgattg agcggtgcaa ggcctctgca ttccacacca actgctgcgg ggaggccagc
     8341 tccctagcct ccgggtccca ccaaagatga tcgccggtaa acagaaaccg gtcccgatgc
     8401 agcagacaga ggctgcccgc cgtatggccg ggaacgggga tgatgcggaa gtcttcgctg
     8461 tacgagcgag gttccggtcc ttcaatgatc acctccgcct cgggcatcgc atggacatcc
     8521 gcacggtgga tgatccgccg ggctccgaac cggcgcgcat acctggcggc atccgccaca
     8581 tcgtcttcat gggtcaaaaa gatctgtgcg aggcctccca tccgatcgaa gacatcgaca
     8641 agcggtttga gataccgcgg cgaatcgatc agccagttgc cgtccggatg ctgcacgaag
     8701 aaactgttcg ccccgaacga cttttccgaa ttgaaaccgc agtagtagac gttcccttca
     8761 atcgggagag ggaagtcggc cttcgcggca ctcaagaggg tttggttcgt atgctcggcc
     8821 ccgatcgacc cggtcgggca ggcgagcaac gcgcgatagg cctgccttct ctccggttcg
     8881 gtggccggct gatgagtcac cgcagaatac tctcccactt cttcgaagct cgacggagcc
     8941 aattgtcggc aggcatcgca attgatgcag gtggaatcga catagaagtt gccggctcga
     9001 ttcgcatcca gtcttttgcg caggtcagcc attcggagct cgcttcctcg cagccctcac
     9061 cacgggcaga gttgttcgca ccactttcgc ccgatccgcc gcgcgccaga gataccaggc
     9121 tgcggccgtg cgatagggcc tccagcgttc tccatacacc agcagctctt ttggagtcgg
     9181 catcgccttt cgtttgtatg cgatccggaa tccgttccgc acgccaaagt catccaccgg
     9241 caggacatcg ggtcggccaa gctgaaaaat cagcagcatc tccacggtcc accgtccgac
     9301 gccgcgcacc gcgatcaacc gttcgacaat ggcatcatcc tcgagccgtt gaatgacggt
     9361 cgtggtcggc acggttccat ccatcgcctt ggccgccaga tcatgcagcg cggcaatctt
     9421 ggcgtgagaa aatcctgtgc cgcgaataat ctccggcgcc atcgccagca ggtcagccgg
     9481 acggggaaag cgcaggccgg ggaacagcgc gatgaaccgc ttgagaatac tctccgccgc
     9541 cttgtcatgc agttgctgat aggcaatggc gcgggccagc gactcaaacg gggaacgcct
     9601 cgctcgaagc gacaacgcat aagggccgac ttcgccgatg acccgcttca tcaccggatc
     9661 gatcttacac aggtgagcaa tttccggtga cggctggttc atacgacttc acctatccaa
     9721 actgcagatg gtggagaacg gccatccagt aattcgtgag gcgtgacacg caaacaggtc
     9781 gcacgtttcc ggaacaaagg tcgcacgtcg cctgacattt cacctttcac gactcacgtt
     9841 ttccggttcc tgaacatgcc gctggcggac atattccgca tacggttagg acacgatcgc
     9901 gtccgcttcg atttcaatca acatatccgg atcgatcaac cgtttcactt ccaccatggt
     9961 ggtcgccggc cggaccgagc cgaagacctc tccatgcgcg cgtccgacct cctgccattg
    10021 atcgatattc gccatgtaga tgcgcgtgcg cacgacgtcc gccatcgacg cgccggcctg
    10081 tacgagcgcc gcctcaatcg tcttcaaggc ctggatcgtc tgcgcgtagg gatcactctt
    10141 gccgacaagc cccgtagccg tcatcgcggt cgtcccggag acctgcaccg actgaccgac
    10201 tcgcaccgcg cgtgaatacc cgattttcgc ttcccatggt ccaccggtcg aaacgttctg
    10261 ccttgccatg aatcctcctt tgatccgcta cggaaaaatt ccaatttaca cagcacgcag
    10321 gatgcgcaaa agggctctcc agcaaggccg cagcgaatgg agagccgaag cgtacccttt
    10381 gcggtacgtt gaggttctac atgaagcgag aacgccgctg gaggccgttt tccgcatcct
    10441 gttacacgtt acatcaccaa cccgccgccg gcctgaatga cctgaccagt cagccaccgt
    10501 gcctgctcac tgaccaaaaa tgccaccaca tcagccacgt cggacggaag cccgagccgt
    10561 ttgaagggtg acgattgtag acccatctcg cggtatggct ccgtcaacac accggtatcg
    10621 gtaaagcccg gcaacacggt attgacggta atgccgcgtg gcgccagttc ctgcgcgagc
    10681 cccttgccat actgttccag cgccgccttg ctccccagat gcgccgtggc gccgggaaac
    10741 ttcaggtggg tgagcgccga cgaaatattc acaatgcgcc caccgtcgct gagcaccttt
    10801 gccgccgcct gcatcgcaaa gtacggcccc ttcgcattca gcgcaagaat ggcatcgaac
    10861 tccgtctccg tcgtctccac caacttcttt ggaatgaacc ggcccgcatt gttgaccaga
    10921 atatcgaggc gatggaactg ctgtaccgtc tcctgcacca ggcgttgcgc gtccgccacc
    10981 cggctcatgt ccgcctggat gacggcagcg gccccgccac tggcctgaat ccctgcagcc
    11041 acctcccggg ctttttcctc atgcacatgg taattcacca ccaccgtcgc gccgtcctgc
    11101 gccaggcgtt cggcgatggc gcggccgatc ccgctggacg acccggtcac aatggccact
    11161 ttgcctcgca gagctttcat ctttttcttc ttttcacttc caacacatca ccgaccagtt
    11221 ctcgggatgc atcgaaccgc tgatgaattt actgtaccaa ttgactcaac tggagatcca
    11281 accggaagtg ttctagtgga tagacttaca gcatgaacca agaacacaga ttggttggga
    11341 atggtagaga agcagcgatg cttagaactc agcgtccttt ggtactttta ataaactcct
    11401 caagatcccc gagccgatga gcccagaaga acacgacggg agtttcctgc gcttcaatga
    11461 gatacttggg tttaaacaac ccctggatag ctgcagctgg aacgatattc tgctcgccaa
    11521 attttctaat ccaactcgcg gcatcgacgg ccacttcttt attgatacgt ttccggctat
    11581 tcagctctga attggacgct ttacattcca gagcaagaat gcggccgtca taaagtcctg
    11641 ccacaatgtc gccattgtga tcacccacct tacaaccgac catgaaatga cctggtttcg
    11701 gagcatgtcc cccaagcaat gtttgaatgc ccaggcgagg agacggaata tgtcgaaacc
    11761 tgaggttcat gagaatgacc cttaccgctc cttcgacggc gtctcggtcc tcagatcgtc
    11821 ttcctgtttg cgtacgctgt gccgaggccg caacggttga ggccaggatg gcggcctctt
    11881 cctcaatcgg cgttggggaa cgattctcat agatccatgg aaatcgttta ggatcaagaa
    11941 taggacgaat gacatcgcga atcatccccg cacgctccgg actagttgca atatgcgtat
    12001 acgccagggt gctatcaatc agcgtaatca gatcggcttc ggaaataggg ggagcgccaa
    12061 gataccgaaa cgcggtgagc agatgttctt cttgtatcag ggatgcaatc agtttgggat
    12121 cgacagggtc agccagaatt ttcggtagca gtgcgactac tttcttattg gcctgttcaa
    12181 gattctcgaa ggctctaaga taaagctctt tgggttcctg gaatcgtttt tggcggaaaa
    12241 tttccctcgc aacctttgca tccttctcga tttgaacatc agaccagtac tttgctccgt
    12301 gcggccatcc catagaagcc ttctatcgct ggcgcatcag ttcgagtggc ggtatgcgaa
    12361 gacgcatgac ttcgcggggt tcaaactttg tgaggccacc ggcgtaggta cggcctgagg
    12421 atatgcggac gttggcattg agccaatcgg ttactcgatc taagtcttcg tctgtgaggg
    12481 gaacgcgcgg ataaagacca tgggcaatat tgacaatccg agctccgcag agatttcgaa
    12541 cgaaggccgg ggggcgacgt gccatatatg tcatcaaaat aggggcggga gcgggcatgt
    12601 ctacacgcca ccatggacgt cggtgttgag cgatgtagct ccgatgagca ccttgactct
    12661 tggcccaaga caagaaatct tgaacacgtt caaatgtaga agcatccaat tcgttcagat
    12721 cggccggaag actcactaca gacttgagac cagcactcga gtctagccga tgatcgggcg
    12781 cattgataag ttcctttgca gctgtgacgg ttgggacccg cagttgacat ggaagctgtt
    12841 gcgcctgttc ccccgcgatc cataccgcat tcatacccgt aacctgacct cggtgaacat
    12901 cgaacagatc accaagttcg atggtatttg ccttttcata aacggtcccg agccctagtt
    12961 gcgaccattt atcgctggct cgtgcccgtg aatggctaat ccttcttcct ccacgaagat
    13021 ccgcaagttc tttgatagag gtaacatggc gcatccgcat cgcgttttcc attgctccaa
    13081 cttggaagca aagaatgacg gcactggtca atgcatcgtc aaagaccggg acagttttat
    13141 caatgagatg aattgcctgc ccacccatac cgttagtgaa catcgtacga aacgaggaac
    13201 catagttgac gtccaaccat tccgcagcag taatgaaaca gccgcaatcc cctggtcgag
    13261 ccagctgtcg cgtcttgaga agaaaatgca gatgaagccc agccaactga ctgcctgaaa
    13321 gatcgtacgc tttgagcgca gctgtatacc aacgcttcca ttgggcttcg atggcatgat
    13381 gacggacata gggcgggtta ccgacaaaga gcgtagcccc cgtgacattc ggaagttcca
    13441 aagatcgata gtcttgaaca acgacgcgaa tctgatgtgc taggcccgca gcaataaccg
    13501 tggctttgag cagatgtgcg atgtgcggtt ccttctcaac ggcaataatt tctgccttcg
    13561 gaaaagctcg agctgccgca agggcaaacc ggcctgaccc agcgccgggg tcaattactc
    13621 gcacaaactg tcgatgttga gatttggccc aggctagcat cgcccgaaca atcgcaggcg
    13681 gcgtaaaggt cgcgccgtac tgccggcgaa catctgggga cacaagtctg gtgtaggtct
    13741 ctccgagagg gtcgccgtgc tgtaaaatac attcacgtat atgctgaacg tgagtactgc
    13801 ctttccccac cttctcctgt gaaagaaatt cgtcggccag ttttgcgaga gcagtgtagt
    13861 caagaatcgg ctcgggccgt gcagctggat caattagggg tggtcggtgc attgtgtgat
    13921 tgctttgccg tatgtgccat tcctttatgc ttgccagaaa acagaaagtc aataaaattg
    13981 taaccaacgg ttacgcttca aatcgtcagc ttctcatgca cgacagggaa tcggcagagg
    14041 gtgataatct tcgatatttt ccatcgctcc cgttcgaaac cgaccggttc gacctcgcac
    14101 tgtcgtcgca ctttctcttt ctctactcgg accacttcga tgcggcgttt cacctcgctg
    14161 ccattcgagc gctgctgcgc gtcgcgcggg aagtgagaat tttccctctg ctcgcgctcc
    14221 gcgctgagcg gtcacagcac ctggagccgg tgtgtgagca actccgtcaa gagggccatc
    14281 acctctcgat cgaacgggtg gggtatgagc ttcaacgcgg cggcaacgag atgctgcgcc
    14341 tacggcgagc gtaaccgggg accatcacct ggtcggccac tcgggcgggc cattccacct
    14401 tccgtgagac acgaactcgg tcacagcctt ccgctcccgc ggtttcaatt cccgctcgcg
    14461 cagatagtcc ggcaacgatg ccggggcgcc cgggtatccg acggcgatca tcgccaccgg
    14521 ttcatacccg gcgggaatgc cgagatcggt gcgggccttc tctatctcaa atcccgccat
    14581 ttggtgagtc accaatccca gcgccgtcgc ttgcaagcag aggctgagcg cggccatccc
    14641 cgtgtcgtgc caggcatgcc ggtttggtgt tccttcgtcc tcaaaattca gccgtgcgac
    14701 ggacaatatc aagaccgggg cgcgaaacgc ccacttcctg ttgccctcct tgaggcaggc
    14761 cacgagacgg tcgtgcgcgg aaggatccgc tttcgttccg acaatgaagt gccagggctg
    14821 ctcgttattc gacgacggcg cccacctcgc cgcctcgaac aggctctgca gttgttccgg
    14881 ctccaccatc cggtccgcaa acgcgcgcgg actccatcgc cgctgtaaca gagcatgaat
    14941 ggggtgggtc acatcagcag gtttttccat gaacgaatcc ttctccggat cggaatttac
    15001 accaacgttc gcacaatgcg ggctggattg ccgacgacaa ggctatccgg aggcacgttg
    15061 tgcacgacca cgctgcctgc tcccacgatg ctacgatctc cgatcgtgac gcccggcagc
    15121 accagcgccc cgccgccgat ccacacatcg ttgccgatcc gaatcgcgct tgcggattcc
    15181 aggccggagc gacgcacctt ggcatcgatg ggatgttgcg ccgtatagag ttgcacggca
    15241 gggccgatct gtacatcatc tccaatggcg ataggagcac agtcaagaaa gatgcagtga
    15301 taattgatga agacgttccg gccgaggctg atgttgtaac cgtagtcgca cgtgaaggtg
    15361 ggaaggacca cggttccttc gccgacggca cttaggaaat gccgaagcag ggtcgtacgc
    15421 atatcgtgac cactgtccgg caacccgttg tacctggcca gtcgctgctg ggcatggcgt
    15481 cgctcatcga gcaactcttt atcgcttgca cgatagagct cccctcccag catcttgttt
    15541 ttttcggtct tctcggccat caactcgacg ctttcgacaa gttacacggg tggttcaggc
    15601 cgtacgccac attcgtccag gtggcgtaag aggtccgctg gatcatcata ggtgcgatag
    15661 gcgcccgcgc gttccagttc ttccctccca taacctcccg acaacagccc caccccgagc
    15721 gcggaagcac gacgggccgc caagagatcc cacacgctgt caccgacgac gatgcacctg
    15781 gcgatgggca tatggagctg ctccgcggca gccagaaaca aatcaggatc gggtttcgcg
    15841 cgcttcacct gatcacgggt caccacggga gttccgggag gaagcttcag cagtttcaac
    15901 gtatgttgcg cactttgcag tcgcccgctg gtggcgatgg catagggcac atgagccgcc
    15961 gtcaatgcgt tcaataattc ttgcgccccg ggcagcactc ggagtgatgg ggcctgtttc
    16021 gcaaaggcgt cggcatgcac ccgctgaatg cgctccgcct cgtcttttga aacgggccgt
    16081 ccggtttccc gccagagcgc atgtaacatg aggccaccgc tcatgccgat ttgacggtga
    16141 atcctccaaa ccggcagttc aattcctgcc gcttgggtgg cctcacgcca agccagcaca
    16201 tgctggtaaa cactatccac gagcgtgccg tccagatcga agagaaacgc tacagagtca
    16261 ggatcctgcg aaacggtcat gaggtctagt tctccaactt cgcatccatg gtgatcgtgg
    16321 cgttgagcag ccgtgaaata ggacagccgg ccttggccgc cgctgtcgct ttctcccaac
    16381 tggcggcatc ggccttgggc accttgcccg tgacagacaa atgcacgccc gtgacggtcc
    16441 agcccgcctc taccttttcc atcgtgacgg tggcagtggt cgccagttgc tcagcgacca
    16501 gattcgccgc gcccaactgt ccggaaaggg ccatcgtaaa acagccggcg tgggccgccg
    16561 cgatcagctc ttccgggttc gtcccttttc cattctcaaa tctcgtactg aacgaatatt
    16621 gcgtctcgct caagacacca ctgtccgtcg agaccgtccc tttgccggtt ttgagatctc
    16681 cttgccattt tgctgaagca ttccgtttca tgcgatcctc ctatcgtgaa tcgttgatgg
    16741 tcaatgccgc gcgaccacag gcctcgccga tcggatcaat cggccaggca ctttccctgg
    16801 ttgcctttct ccttatccgg atcgacgcct cgattcctgc catgcgcccc agccggatcc
    16861 tgtccgatgg gcgacgtaga ggtggttcga agtgatctct tggatcggcc catgactcaa
    16921 ctcaatggtc attggttcgc cggtccgcag atcgacggtc acctgtcccg gctcgtgatc
    16981 gatcgtcaca gagacgctcc gacctctcca ttgcagcggg aacgacaacc gatcccagtt
    17041 cgatggaagg tgcgggtcga tgacaagccc atcttctcga agcctgacac cccccatgcc
    17101 gaagacagcg gcctgccaga gccccccgag ggtagcgatg tgaactcctc ccgccgcatt
    17161 acccctgtga ttggaaagat cgatcgccgc agcgtcccga aaataacgcg cggccaggtc
    17221 ggaatcgccg agcctggcgc tcactaaggc atgaagggcg ggacttaagg agctgccatg
    17281 tgcggtacgg ggttcataat aacgaaagtt cgcctcatgc actgcagacg ggaatcgttc
    17341 ccagaggagc gcgctcagcg cgaccacgtc cgcctgcttg accactttgg acttttgcgt
    17401 cctgtcgcgc ccgagcgtca catcgatggg aacaggcagc gtgcggtacg cctggagttc
    17461 gacctcctcc agttgaaaga acccggaaaa ttgctcgaac agattggtcg gccggtcgaa
    17521 tccggtggtc atgagctccg cggtccgacg ccaggaatcc ggttcatcgg gcttgacctg
    17581 caagcgctcg catacgatgg gccagtcatc aggacggcgc gagcggagcc agtcgaccgt
    17641 gtccgcggcc cgctccaggt tccattgcgc catgacattg gtatacgcat tgtcatccac
    17701 ggattcatgg tattcatccg gcccgatgac cgttcgaatg tgatactgcc cgtccggctc
    17761 taaccggcca cgactcgccc agaatcgcgc ggtctcgatg agaatgtccg cacctgccgc
    17821 gagaaaaaag gcctcatccg ccgtcgcctg ccagtattgc cagaccgcat aggcgacatc
    17881 ggcgctgata tggtgctcct gaatccctgt gagcacttga accacccggc catccggcgc
    17941 gatgaccgta tccggcgtga catcttctcc ggtatcggcg gactcccagg cgaacaacgc
    18001 tccctgatat cccagccgcc gcgccctgtc gcgggcagcc ggaagagtat ggaaacggta
    18061 ctgcaagaga gcccgcgccg ccggaggatc ggtaaaagtg taaaacggca gcagatagat
    18121 ttccgtatcc cagaagacgt gccctttgta ggtggagccg gtgagagccc tggctccgat
    18181 ggacgcatac tcgtccttcg gattcacggc gctattgaga tgatagaggg caaagcggag
    18241 cgcgcgctgc gccatctcat cgccggctac ctccactccg ctggcgcgcc aacgatcctc
    18301 ccaggcttcc gcatgttgca gcatgtgggc attcagactc gcctcggaga ctcgccgcac
    18361 atgttctccg gctgcggcag cgggatcctc tacctcgcgg gatgaataga cgacaaccga
    18421 gcgatcgaac cgggccgcct cgcccctttg ggcctgccac gtccactgct cagcaaatcc
    18481 ttcgaccggg ctattgcccg ctccctcctg actgttctca tgtcgcagct tgttatgaga
    18541 tgccatggcg atcgtcgttc ccctgtccgt ccgcctcacg atcacggctg cctcgccctc
    18601 caccctggaa gaccattgtg tccccggctg aggcccttca gtgaaagatt cgatcgagat
    18661 ggtaccggaa taattctctg gcgtgatcgt gaccgattgg agcaggaggt gtcgatcgtg
    18721 atgacacacg gcgcgcacgc aggtgagggc cgtcactcgg ccctgctcat cctcgtgccg
    18781 ccaggttcga aaaaacagac catgctgcaa atcaaggatg cgtcggtgat cgagcgtggt
    18841 tccggtttcg agcgacagcg ggaccccttc caccagcacg cgaagctgcg tccaatccgg
    18901 gagcttcgtg agctccaggg tacctcctgg ggcctcagag gcctcaaacg ctccggccag
    18961 gaaggtggca ggggaggaga ggctcgtgcg ttcggcgagc gaaccccgcg tccccacata
    19021 gccgttcgcc atcgtaaaga tcgactcgat ctcatgctcc caccccggct cgaaccctga
    19081 ttcgaccaga acccagcttg ggtcaatggc gaagggcaga ttggccgaca ataatggggg
    19141 agccggtttc gcctcttctt ctccttgatc cggtcctgac aggatcgatt tgtcgcgagg
    19201 tatccacatc ttgacgttcc ttcacatctc cgaggggcag aatggtagca tgcagaggcc
    19261 atcacctatc ccgtatgccg acgcacagac cgacacgcaa gggagatcga ggatgacaca
    19321 aggcgcaacc ggagaacaac cgttccatcc atggtccatt gatctgacca agttcgatgc
    19381 ggtgctcttc gatcttgacg gagtcctcac aaaaaccgcc gtcgtgcatg cagccgcctg
    19441 gaaacgctta ttcgatgagt acctgcaggc gaaagcttcc cgcgaacagg tcccttttcg
    19501 accctttgat gtcatgctcg actatcagcg atacgttgac ggcaagctcc ggtacgacgg
    19561 ggtacgggcc tttctcgaat ctcgccatat cgtcctgccc aatggcaccg tccacgatgg
    19621 cccggagcag gaaacggtcc aggggctcgg caacaagaag gacgggtact tccaagctca
    19681 tctcaaacag catggcatcg agctgtatga cgacgcggtg cgatttcttc ggacccttcg
    19741 agcacagggg gtgcggacag cggtcgtctc agccagcaag cacaccgcgg ccgtcctaca
    19801 aaagaccggc ctcgcggact acttcgatac tcgagttgac ggcatcgaaa gcgaacgact
    19861 ccatcttgcg gggaagccgg caccggatgg tttcctcgaa gcggcacggc gactgcaggt
    19921 ggatccaccg cgagccattg tcgtggaaga cgccgttgcg ggcgtgcagg ccgggcgcaa
    19981 cggcggattt ggattggtca tcggggtcga tcggaccggc catgcggagg agctcacacg
    20041 aaacggtgcg catctcgtga cgtccgactt ggctgaactg tgcgtatagc tcctgacatc
    20101 gccgcgtccg actggatcaa cagatctgat gaccgcacct cgactcgcag gtcccgaaca
    20161 aatccattcc gattcggcat ctgcagaatg ggggcgggga ggccgagacc tccccgctcc
    20221 gagctgccgt tattgcacct ctaccgtgat cgtatcggtc gcggccaaat ggtcgtggtc
    20281 atgggttgcg gcttggacct tgatctcatg tttgcccttg ttgagacctt tgaatgtgcc
    20341 gggaaacttc ttctgcggtt cgccgtccag atacacatgc ccatgagccg ccttggaacc
    20401 tttggtgagt tcataggtga gctcgaaggt ctctcccact tgtgccccgt ctttcggcga
    20461 ggtgattgtg atgtgtgatc cgtcatcgac ctcggcgaac aggggaccgg ccgaaaaaat
    20521 caggccggcc gtgaacaacg tggcgaaaaa tcccttcatg tgtcgcatcg caccctcctt
    20581 ggttgggctc tggcgatcgt ccattgaccg ctcagtgttc atcaccagcg cgcttgtccc
    20641 tcgatggtga tcgtcagttc gactcatcgc ttagtgctct ccgtagcgtt ccatccactt
    20701 gcgaaggtgc tccacggctt ctgccttctg atccggccgc agggtcgcat gccactcggc
    20761 caccttcggc aacacgcgct gcatcaagcg ggtctgtcct gcctggtgcc gctcaaacaa
    20821 ttgggccacc ttggactgat ccagccgatc actctggact tgcacgatca cgtcctccat
    20881 gatgacccgg tgctcttgtt gtgactcggc tcgggcagcg atcacttcgt ctttcacgac
    20941 ggccagcttc gccttctgct ggttatccag gtccaagtgt ttcgcgatct tgcccgtcat
    21001 ccagtcagcc cgctccggcc aggaccagcg ttccgacgac caccctgaat gtgttacgca
    21061 tactcatacg atcctccctt tggcgtgatc tcacacagtc agtactcgaa ggccacgcga
    21121 tagagcgcgg gagtcatttc aacccccagt tctccgagct tggccttcat cggcgtgacg
    21181 ctcttttcgt aggccttcca gtacacctct tcgttctccc agatcgctac gttgatgtag
    21241 ttaaattggc tgtccggctt gatgcttttg tggaatttgc cgctgatgaa cccctcctgc
    21301 ttcgtgatgt gctccttgac cccctgccac cactgcacaa attctgcttc tttggtgtga
    21361 gggactgaaa acacattgat caacgtaacc ggcatggcat gcctccttga atatgttcga
    21421 ttttgacgtc caccttcacc tgacactctg tgagacaggg aatattgatc gcccgcccca
    21481 gtgcggtctg gcgagagcag ggcactcgcc agacctattc aggttactgc gcctttctca
    21541 ggtcgatgac ggttccttct tcgttcagct cgacgacgat attgtctccg tcggcgatcg
    21601 gcttggtctt gatttccatc cgctccagcg ggaacacttt ctccccctcc ggcgtggcga
    21661 gcttgatctg attcttgttg ggccccgtac ggatcaactt gccgaagatc agccgatggg
    21721 tcccggcctt ggccttatct tttccatgca catcgatcac catgccctct tcattgatcc
    21781 agagggacac gtgatcgccg accgccgcat cggacggggc atacttcttc aagagcgtgt
    21841 actgtccggc cggcgtcttc acgtaggcca ggctcgcatc aaacttggtg accgtgccgt
    21901 ccacctgaat gacataaccg ggcttcgtgc gcgggtgctc gtcaaagctc atctctaccg
    21961 tgaagcgatg gatgtcgatc atctttcccg cttcgttcaa ctcgatggtc accggtgccc
    22021 cttcttccaa ggcggacaac ttggactttc cggtttggac atcgatggcc ttctctcctt
    22081 ccggcgtcca cactttgatt tctttcttat ccggcgaggt ataggccagg ttgccggtca
    22141 cgaagcgatg ggaatgggcc ttgtctccct tgcgatggac atcgatcacg gcattgtttt
    22201 cattgacctg catgtccact tcttccccga cctcaaggtt cttgggcgtg agcgtcgacg
    22261 cgattttcat cgtcccccag ggggtcttca cggtggtgat gcctgactgc acattcgaca
    22321 cgaccccgct gaccttcatg tgcgtggcct tcttgccccc gccttcggcg gcgaatccat
    22381 agggcaccgc catcgacaag ctgagaacca ttgaaccgaa aataaccatt gtgcgtgtca
    22441 tcgtttgact cctttcatcc gtgattgtga acgcctgtct tgcctaaatt ctgcacgatg
    22501 gccctggtca ggcagcccga tgcctgaatc gtgaccccca tgcggtacat ctgggacaga
    22561 agcgtcgtgc ccgcctcatc gataaaggtc acttcccgga gatcgacgtg gcatgggacc
    22621 ggcccgtcga actcctgctt caaccaacaa tcccgcagct cccggaccca gggacccgcc
    22681 aaccgcccgg aaagaacgaa ggtggttccc gggttcgtat tcgacaccgt aatcttcagc
    22741 atgatcgtgg accccttcgt cccttgagtc cggccgggcc gatgcccggc cccaccatga
    22801 cgctcagaac gtgcgcagag cgaaactcgc ttcccgcgcc ttgagagccg gggattcctc
    22861 accaagccac cgggcgatta cgccgtccag cgaccacttg ccgccaccga cgaccagcag
    22921 cacaaggctc atggcgatca ccaggagatg gaactcaaaa ccctcacctt gctgctgccc
    22981 gaaccagttc atgaagaacc cctgcggcaa gtgcaccgtc atgatcgcgc cgagcataat
    23041 cacaatgaag ctggccgccg tgaagcgcgt cagcaatccg gcaatcaggg ccagactccc
    23101 gatggattcg ccgatgatga ccaggaacgc cacgatccat ggcaagtgca tgacattggt
    23161 aaagaagccc atcgtgccgt cgaagccatt cccaccgaac cagcccaaca atttttgggc
    23221 accgtgggcg aagatcactc cacccaacac cactcgaaga atcaaacccg cccaggactc
    23281 atccgttctg aaaaagctgt acatgtcaca cctccttgat atgaattacc gtcggtggct
    23341 ccgcacccgt tgcggaaccg cactcgccat tcgatctttc aggaacaacc ctgccacgaa
    23401 gagcggcagg gtcacgagca ttccgtacca ccatccccac agcgtcatgt ctttcatggc
    23461 ctcacctcct aatccctcat gcagcgctac ttcaggtaag ggcaaaggtc attccacctc
    23521 gctgcctgtg ctgtcgatat ctgtaaccaa ttgaaacaac gggcaaacct tgtcgatcac
    23581 caacaggccg catccgcatg agtcattgag tgacgatgcg acggtgacga gggttcgcca
    23641 ggtgacgaag gagatcgtca gggagggcag agaacaggtc gaggattacg cttcgggatt
    23701 tttggggtcg taggttgtcg cgcctctgcg ccatgggacc catggcgcag agacggttgg
    23761 tcggtcggct agaggatggg gctcaccgat cgctgaactc gaagatgtcg tgcgcgtcgg
    23821 tcacgacatg catcccggta atgccggcag attgaatatc cccactcgcc agatgcaacc
    23881 ggtggcccac aaccgcaccg gccaagccgt gcctgggcat cggcatcatc ggaagctcgg
    23941 accagctgtt cgtcgccgga tcgtacgctt ccaatgcgcg gaatgcggcc atgagatgtc
    24001 cgtcctgatg ttctccacca gccacgtaga tgcgcccgtt gtagaccccc caggcacctc
    24061 cgctccgctc ggtgggcatg cgggccctga ccagacccca ctgatcggtc gccggatcgt
    24121 attcctcgac cacattggtg ttgctggcgg tgaaaatgaa ggcgcttccc aggcggccgc
    24181 cgatgacgta gatcttgttg tttacggcac cgacggcggc gtggttgcgg gctgtcggca
    24241 tagacgagcg ttcggaccag ctgttggtct tgggatcata ttcttctacg gtgcccaccg
    24301 accgatgggg tcgcgtcgga tagagagcgg cttccttgga accaggatgc atcgctccgc
    24361 caccgatcac atagatcttc ccgttgacca tccccgcaac cgctgacccg cgcgcggatg
    24421 gcatggccgc caaggccttc caactgtcgt gggccgggtc atactcccag gcgttgttga
    24481 tcggcaccca ggccggaggt cccgactgtg gcggcacaaa gccgccgaag gcgtagaact
    24541 ttccgttgag ttcagtgaac gccacatgat gcgccggcag cgccatcggc ttgttcttgg
    24601 tccatttgtt agccgacgga tcgtattcgt acacaaggcc ttgggggatc cagccgggcc
    24661 cgagtccacc gaacacgtag aattttccgc cggtcgacag gccgaccaac tcttcggacg
    24721 gctccgggaa cggcgcacct ttgctccacg cacccggcgt gtccgcctgg gcaggagcga
    24781 ccatcatgag cagactcacc atgaccatga ccaatagcgt gtgcataaga tgcctccatg
    24841 aagggcagac cgagcgatgg atgaaccaca gctgccctgt tgtcttccag tggttggtga
    24901 ttgtgttgcg ctctcgaccg ttcgccccct ctgtgatcgg tctatgcttc acgtccatcc
    24961 gatccatcgc gcccgactgt cagaaagtgg cacgcgggtg gcgggggtat tggctcattg
    25021 gaattgccgt cggaatcgcc acattccaaa cccgaacaac gctcctccca acaggagcat
    25081 ggcgacgacg ggcttccaga gcaggtccag gcctgctccc ttcagcatca cgccgtaggt
    25141 gatgtcgatg tagtaccgca aaggggagaa cgtcatgacc gtctgcaccc actgaggcat
    25201 cgattcgtac ggcgaggtga ggcccgacaa caacagcatc gggcccacga cgaagagcgt
    25261 catcatgccc acttgcgcct ggttcttcgc catggtcgcc gccactaacc cgaatcccgc
    25321 cgtcgtgaac acatgcaagg tggtcagcag aaaaaagagc ccggtcgaac ctttcatcgg
    25381 cacgtgaaag acggggtgca acacgccata cagggcgaga ctgagcgcga tgaggatgac
    25441 cccggacatc gcgagtacct tcgaaaacat gatttgaaag ggggacagcg gcgacaccag
    25501 caactgttcc accgtgcctc gttctttttc acggaccagc gcagcagccg gcaacaacac
    25561 cgcaaagatg gtcgtcatgc gtaacacatg tgcgatggac tgaaaccatc gttcatcctg
    25621 ggtaggattg aaccagacgc gatgggcgct ggagacccgt ggtatggctc cctctgctcc
    25681 ggaaagaccc gccgaggcca agcccgtctc ggtcccgaac aggccggcga ttcgcacgct
    25741 atacccggct gccgagagac cttggggtgc attggtggta tcgacgagga gttgcaccgc
    25801 cgtccgttcc ccgctcatca aggcctcgtg aaatcgtggc ggaatctcca acagcaccat
    25861 caccttgccc tggtccagtt gccggagccc ctcgcgagga tcgctgatct ccccggcgaa
    25921 tttgaaatag gggggctgaa accgatggat cagctcgcgg gacgagtgac tatgatccgc
    25981 atcgtgcacc agcaactccg cattggtcag ctgcatcgtg atcccggctc cgctgaccac
    26041 caccgagagt gaaaacgaat acaacagaaa caacaacagc ggcacatcgc gcgacagttg
    26101 caggatttcc ttccacgtca tcgccgcgag ccgccgcccc cacgtgaccg cctgctgcct
    26161 ggccgtcatg cctcacccct catgtcttcg gcctcttggt gaagagccga tacgtaagcc
    26221 cgctcaacgc gacggcaaac atcgccagcg ccagcaggtc ggtccacagc acgtccgcgc
    26281 ccacaccctt cagaaaactt ccgcgcacga tgttggtgta atacatggcg gggtacagat
    26341 gcgcctgcac cttcgcgcct cgagttaacg aggccaccgg caccaagaga ccggaaaaga
    26401 gaatggtcgg gatcatcgcc acgaccatcg tgataatcag cgccgccatt tgcgtctgca
    26461 ccagcagaga aatcagcagg ccgatccccg tggtacagca cacgaacagc accgacgccg
    26521 aaaagaacag gaggaagctt cccttaaagg gcacctgaaa gaggccgacg gccatcagcc
    26581 ataacacgca gacgttcacg agtgagatga cgatataggg aagcagcttg ccggtcagga
    26641 attcggcgcg gctcacggtg gagctataga tattgtaaat cgaacccgtt tctttctccc
    26701 gcacgacccc cagcgccgtc agcaggggcg aggcgagcat cagcgtgaac atgaccagcg
    26761 ccggcaccat ggaccaggta ctccggactt cttcgttgta cagatagcgc acctcgacgg
    26821 tcagcggacg caccagcgca tcggcctgct cctgggtgag accacgggtt cggcgcaggt
    26881 actcgatcag tcgctcctcg gtgaacgcct ggttgatggc aatgacatag cctttggcga
    26941 tatccgtatg gagaggaaag gtcccatcga gcaaggtctg cacggaggcc gattcccccg
    27001 ccgccaactg ctcctggaac cgctcgggaa cgatgatggc cgcccggatc ttcgtctcgg
    27061 tcaacaggcg atcaagggca cgttcatcgt cggcataccc ctgaaacgaa aaatatcgcg
    27121 actgcatgaa ccggtggaga taatcccggc tcaactcgct ccgatcccga tccaggaccg
    27181 cgaacggaat gttttcgaca tccagattca acccgtaccc gaataccacc aaccataggg
    27241 ccggcaacaa aaaggccaac agcaagaaca gtcggtctct ggtggtctcc ttccattctt
    27301 tgagcgccac ggcgctgatt cgttgcagat tcaggctgac acctcctttt gcgccgcctg
    27361 ttccagtgcc atcacccgtg acacaaagac gtcctccaag ctgagcatcc gcgtccgcac
    27421 cgcttcgacc gcaatcccgg agcgggccag gacatcccga agacgttcct cgtcgcgacc
    27481 cggatcacgc gagagcacat ggatcttggt gccgaagagc gccgcgccgg cgaacccggc
    27541 tgccactacg tgcgcgagtg cgatccccgg cttgtcgacg accatctcca cgagttgccc
    27601 gatgtcccgc acgaccccca gcgccgtcag caggggcgag gcgagcatca gcgtgaacat
    27661 gaccagcgcc ggcaccatgg accaggtact ccggacttct tcgttgtaca gatagcgcac
    27721 ctcgacggtc agcggacgca ccagcgcatc ggcctgctcc tgggtgagac cacgggttcg
    27781 gcgcaggtac tcgatcagtc gctcctcggt gaacgcctgg ttgatggcaa tgacatagcc
    27841 tttggcgata tccgtatgga gaggaaaggt cccatcgagc aaggtctgca cggaggccga
    27901 ttcccccgcc gccaactgct cctggaaccg ctcgggaacg atgatggccg cccggatctt
    27961 cgtctcggtc aacaggcgat caagggcacg ttcatcgtcg gcatacccct gaaacgaaaa
    28021 atatcgcgac tgcatgaacc ggtggagata atcccggctc aactcgctcc gatcccgatc
    28081 caggaccgcg aacggaatgt tttcgacatc cagattcaac ccgtacccga ataccaccaa
    28141 ccatagggcc ggcaacaaaa aggccaacag caagaacagt cggtctctgg tggtctcctt
    28201 ccattctttg agcgccacgg cgctgattcg ttgcagattc aggctgacac ctccttttgc
    28261 gccgcctgtt ccagtgccat cacccgtgac acaaagacgt cctccaagct gagcatccgc
    28321 gtccgcaccg cttcgaccgc aatcccggag cgggccagga catcccgaag acgttcctcg
    28381 tcgcgacccg gatcacgcga gagcacatgg atcttggtgc cgaagagcgc cgcgccggcg
    28441 aacccggctg ccactacgtg cgcgagtgcg atccccggct tgtcgacgac catctccacg
    28501 agttgcccga tgtcccgctc gacctgtttt ttcaggtgcg ccggcgtccc ctcggccacg
    28561 atgcgtccgg catacatgag ggccagccga tcgcaatgct ctgcttcgct gaggtagtgc
    28621 gtggtaatga gaatcgcgac tccttcctcg cgggccaacc gtgacagcag ctcccagaac
    28681 cgccgacggc cgatggggtc cactcctgac gtcggttcat ccaaaaacag cacgcgtggg
    28741 ctgtgaacga gtgcgcaacc caacgccaga cgttgccgca cacccatggg aagacgaccc
    28801 gtccgatcat cctcgtagcc ggtcaggccg gccatctcca cgatccactc catccgttgc
    28861 tgcgcctctc gccgagccaa cccgtagata ccggcaaaga gtcggatatt ctcgatgacg
    28921 gtcaggtcca gatagaggga gaacgcttgc gacatgtagc cgatgcgttc tttgatcgcg
    28981 ccgcccgccg tgcgcatatc cgcgccggcc acacgtccct ctccggccgt cggaggcaca
    29041 atcccggtca gcatcttgat gacggtcgtc ttgcccgcgc cgttcgcacc cagcaacccg
    29101 aagatttcac cttgcttcat gtcaaagctg acccgatcca ctgctcgaaa cgaaccgaag
    29161 tcccgaatga gctccttggc ctcgacggca agtccgtcga gctgctgcct gcccgcgggg
    29221 cgagcggagt agtccgatcc tgccgacact cccggctgtt cttctcgctt cttcaagagc
    29281 agcgccacca cgacatcttc caactccggc tcctccgtgt ggatgtgctc aaccggtaaa
    29341 ttttccagcg cctgccggat ccccgcgacg gcggcttccg gctcccgttc tggtgtgaac
    29401 acgtgcagca aggggcccat ggattcgact tgggcatacc ggcgtttcag gcgggcaacc
    29461 gcttccagtt gcgggctcga ttcgaaggtg acgacaaggc ccgggaccag tgattggact
    29521 tctgcaggag tgcccgatgc cagcacccgt ccatgagaga gaaacgcgag ccggtgaaaa
    29581 cgagccgctt cgtccatgta ggcggtcgac accagcgcgg tcatgccctt gtccgcctgc
    29641 cattcggcga gaatggccca gaagtcccgc cgggagacag gatcgacacc ggtcgtcggc
    29701 tcgtccagaa tggccagctc cggctcatgg atcagcgtac agatcaagcc gagcttttgc
    29761 ttcattccgc ccgagagctg tttcatcagc cggctgcgga agcgatccag gcgggtgatc
    29821 gccaacagcc gggacttgcg cgcgcgaagc tcaggctccg ggaccagccg cagcctggca
    29881 aagaagtcga tgttttcttc aacggagagc tcggggtaga ggttcagccc cagtccttgc
    29941 gggagaaatc cgatccgcgg tttcacccgt tccgccgccc gctcggaatc cacgagggtc
    30001 ccgaacactt cggcggatcc cccgtcgtat cgcaacaccc cggcgatcgc tttcatcaca
    30061 ctgctcttgc ccgcaccatc cggtccgatg aggccataga tttcacccct cttcacggtg
    30121 aggtccaggc ggtcgaccgc aagatgctgc ttgtaactct tgacgaagcc ggacacacgg
    30181 acaaccacct ccggtgacga tgcagcgggc ttcgtcatcg aggtttcgac caggccacgt
    30241 cgtccttcca gcggatcacc gcgtccgccg gcagacccgg cgtcagccga tggtcaggat
    30301 tctcggtgag atagagccga acggcataga tcagcttgac ccgttcgtcc ggagtctgga
    30361 cttctttcgg cgtgaattcc gctttcgaag cgatgtaccg gaccgtcgcc tcgaacgacc
    30421 ggtcggaaaa cgcgtccgta tggatgcggg cgggcaaatc gaggcggact ttaccgattt
    30481 ggagttccgg aacatagacc tgcaggtaga ggcggtcgag atccaccaat tcgagcaggg
    30541 gcgcgccggt tgccacgacc tctccgacgt ccaccatccg agtggtcacc gtgccgttcg
    30601 tcggagccac gatcgtcaga tcgtcgagta tgctacgggc ctccgcgaac gtcgcatctg
    30661 cttggtcgcg ttgccgttcg agcgccgcga cctccgcttc tttcgatcgt attcgtcccc
    30721 accccaattc ggcctgcgcg agttccttgc ttgcttggtc aagggcggct ttcgccacgg
    30781 caatctccgt gacagccacc ttccagcgcg cctcagcctg atccacctgc tgctgcgaca
    30841 ccgcctgctc gggaagcagg gctcggatgc ggtcggtgtc ccggcgggct tcatattcga
    30901 ccgcctgtgc cttccggatg acggcgcgca tgcttgcgac tttggcggcc actgcttcga
    30961 tgctcagagg cacttcgagg ttcaggaccg caagcgcggt atgggccgct tccacctgcg
    31021 ccatcatgct gtccaccagc cgtgccgcct gatcgacctt cgctctcgtt tgggcgtcgt
    31081 ccagccgaat cagcacgtgg cctttgtgaa cctggctccc ttcgcgcgcc gccaggtcta
    31141 cgatgcgccc gggaaacttg ctggaaacgg tgacgtggtc tccttcaatc cgcccattgg
    31201 cctggatcag tccctccggg aggccgtcct gccacaccag ccgatcgagg atcacgtatc
    31261 cggcgatcag caccgacgca aggacggccg ccgcaatggt cgaggtccga agggtcacga
    31321 ctcagtggtc tccgtaccgg tccatccact tgcgaaggtg ctccacggct tcggccttct
    31381 gttcaggccg caaggtcgca tgccattccg ccaatttggg caacacgcgc tgcatcaggc
    31441 gggtctgtcc cgcctggtgc tgatcaaaca gccgggccag cttggcctga tccagccggt
    31501 cactctggac ctgagcgatg acctcttcca tggtcgcctt gcgctccttc tccgactccg
    31561 cgcgtacggc catcgcttcg tccttcacgg cggccagctt ggccttctgt tgttcatcca
    31621 ggtccagatg tttagcgatc ttgcccgtca tccagtcggc tcgctccgtc ggcgtatggt
    31681 gtcggtgaca accggcaccg acaagcgccc cggcaatgac caacgtcccg atcgccaccc
    31741 tgaccgtgct ctgcatcttc atgctgccct ccttggtgat tggcgtgata tacgagcgac
    31801 tcttcccctc acattgaatc aggagtcgga ggggacatgg tagggacccc caccattatt
    31861 ctctagggcg agagaggccc agcttcttga ttttcgaggt gagtgtcgta cgcttcaggc
    31921 ccaagcgagc ggccgcccca tccggtccgc cgatgaccca atgcgtgtcg cgcaagacgc
    31981 gcaggacctg ctcgcgttcc gcatcctgca gcgtaggggt aggcgccgga gcctgccgcg
    32041 cctcaatttt gagttccaag agcggcacct gcaagtgggt tccctgggtc agaatcaccg
    32101 cgcgctcgac cagattctcc agttcccgga tgtttcccgg ccagtgatag cgggacagag
    32161 cctccaacga cggcccggga atcgtttcga tgtgcttctt catgcgggca gcataatgct
    32221 gcgtgaaata gcgcacgagc accggaatat cctctcgacg ctcgcgaagc ggcggcaggg
    32281 taatcggaaa cacgttcagc cggtagtata aatcgcttcg gaactcatgg tcttccacca
    32341 ttttggccag atcccggttc gtggccgcga tcagcctgat atccacgtgg atggtgcgtg
    32401 tgctgccgag ccgctcgaat tcctgttcct ggaggacacg gagcagcttg gactgcagct
    32461 ccaacgggat ttctcccacc tcatccagaa agatcgtccc gccatgcgcc aattcgaatc
    32521 ggccgacttt ctgggcgatg gccccggtga aggcaccccg ttcatgtccg aacagctcac
    32581 tttccagcaa cccggtcgga atcgcggcac agttgagctt cacgaaggtc cgctggctac
    32641 ggccgctgag ccggtgaatc gccctggcaa tcagttcttt ccctgtaccg gtttcaccct
    32701 gaatcaacac ggtcgcctgg gtcggcgcca cgatctcgac ttgccccaga acctgcttca
    32761 gcgcgcgact gtcgccgatg atgtcgtcga acttgtgctc aaggcgaatt tcttcttcca
    32821 gatacaactt ctcttcggtc agtcgatctt tgagttcggt gatctcctga taggcgaggg
    32881 cattgtccac cgcgagggcg atctgtccgg ccacctgact cagaaactcg acgtcctgtt
    32941 cgccgaacgc ctgctcctgc cgactggcca gattgaggca gccgatcacg cggtcttgcg
    33001 acaacagggg caaggagcac atggagtgga agcctttcat gaccaggagc tgaggcacgg
    33061 cgtgcgaaaa tgccgtcagg tccggccgat tgttggcgac cgcggggcgc ctccgctcca
    33121 acgccaggcc ggccagagaa ccggcaacat cggacaccgc cccctcggtc aacgcgccct
    33181 ggttttcagg aaaatccaag gcgtggaggc ggacgcggcc gttcttgccg tcgcacagaa
    33241 tcaggctggc atactcttgc ggcaccatct cgcgcagaca gagactcact gcgctgaata
    33301 cctcccgcag atcgagtttg gacaccatcg tattggtcat gcgaagcagc agactgaatc
    33361 gatcccgctg ccgttccaca tcttgcctgg ccgccgccgc cgcttcgcgg ttcagcacat
    33421 tttccacggc caccgccacc tgccgaccga tctgttgcat cagtgcaaga tcagagactc
    33481 tgtacgcatc tttcttcaga cttgaaaatg ccaacgcgcc caattggcga acggcggagg
    33541 tcaagggcac cagacagcag gactgcacgc catcctcgcg catcaacgcg atgagtttcg
    33601 gccaccgatg ttcttcggcc agatcgctca ggatcaccgg ctgctgggtc tgccacacca
    33661 gaccggccgg cgtgtcggcg ggatgggatt cctgaccgcc gataatgtcg gccgggacgt
    33721 tcgctcgcaa cacatgaagc cgcatcatgt cgcgttgcgc atcgtagagg gagaggccga
    33781 caaagttgac gggcacgagc agcggcagca ggcgggcgag atcctgcacc aacccctgca
    33841 agtcagcatg gctcgcaatg gcttccacca cctgcagcag agcgccatat cgctcactgt
    33901 ttccttgcat cgcctgtggg gttggtaggg atggccggtc gttcatgagt cgtaacctag
    33961 tatgaccccg accggcacag tacgcaacgg caccggggag tactctcgcc tccgctcttc
    34021 cgagacggaa actcccggag cggcgagcaa tgccatcgtc ctcgtcacct catgctcacc
    34081 atcgaacctc ttgccggtcc ttaatgcccg cctcctccga tgtcggcccg gtgcatgtcg
    34141 atgaccgttc ccgcttcatt cagttcaaca gtgaccaatt cgccttcttc cattcccttt
    34201 gtcttctgtc cttcttccgc cagcggaaac acttgctcgc cggcaggcgt ttgcagcttg
    34261 atctgctttt tcatccgacc gacgtggatg agtttaccgg tcacctgtcg atgcgctcct
    34321 tccttccctt tgaggtgcat gtcaacgatg tagttgttct catccaacac aacgatcacg
    34381 tcatcccccg ctttgaacgg ctcctgcccg tgacgccgcg ccatattttc attgagctga
    34441 tgtatcgcac cgttcggtgt cgtcaccacg agcgcaccgg cttttttcgt cacgacgctg
    34501 gtgaattccg tgtggccgcg cgcaagatgc tccgccccga tcgccgcatg ggcgacaggt
    34561 gaggcaatgg agagcgcggc cagtaccgtc gccaatgcga gacatcgaaa cgtctgacga
    34621 ttcatgatgg tctccctgcg gtctcgaaca tggtgtcttg ctgttatgac ccgagacgca
    34681 cgatcggctt gatcgtgatg cccttctcgc tgtcttccgc cgcctgattg atctgatcga
    34741 gggggtaaaa tttcaccagt ttgtcgaacg ggaaacgccc ctgctggtag agttcgacga
    34801 ggcttggaat aaacagatcc ggcacgctat caccttcgat gatgccgata atccgcttgc
    34861 cggtcgtcat gactccgttc acatcgaaac tggcttcggt gcccaacgcg ggagcgccga
    34921 cgatgccgca cacacctttg atcgccaagg cgtcgatggc ctggcgcagg actgccggcc
    34981 gcccgctcga ttcgagcgtg aaatcggcgc cgccgccggt gatcttgcgc acggcctcga
    35041 caggatcggt ctcgcggctg ttcaccgtat gggtggctcc gagctccttg gccagtgcaa
    35101 gccgcgatgg gacaacatcg gcggcaatga tggtggtcgc accggccaca cgcgcggcca
    35161 tcacagcggt caagccgacg gctccgccgc cgaacgcggc gaagctgctg cctggcctga
    35221 ccttcaaggc gttcatgacg gcgcccgcac cggtttggat gccgcagccg agaggaccga
    35281 gtaattcaag cggcgcagcg gccgggacct tgacgacgtt gcgctcgtgg gcgagcgcat
    35341 aggtgccgaa cgaggactgc ccgaagaaat gatcgtgcac cgctccctgc ttgtcttgcg
    35401 tcgcggtgct gccgtcctca cgggcgcccc cgaagttcaa cgggtaaaaa ttctcgcaat
    35461 aggtcaagtc gccgttcagg cagggcttgc agtgaccgca ccacatgtag gtcaacacga
    35521 catggtcacc cggtgcgatt tttttgacgc tgctgccgac ccgttccacg ataccggccc
    35581 cttcatggcc aaggacaacc ggtaacggca cggtgtagag ctgatcgcgc gcgaccatgt
    35641 cggtgtgaca catgccggtc gcaacgatcc gaatcaaggc ctcgtcggga cgcggctctt
    35701 caagagtgag cgtctcgacc tggaacggcc cgcccttttt gcggaccacc gccgctgtga
    35761 tctctcgggt ctgtatcctg ggtatgttgg tcatctgtta ctccttcatg cgcgatcgtc
    35821 atcgcatcga ctgaacagaa cgagtcgccg ggctatcgaa tgccggattt gcccggcgca
    35881 agaatcccat tgggatcgag cgccttcttg agcgtcttat ggacgtggcg ttgcaccgga
    35941 ccataggtgt gcgcgacctt atccatgaag gccgtgttga cccggtaggt gccgtaacct
    36001 tcatttgaaa actctgtcag caactcatcg aaacactggt aggcggcctt cgtcatggcc
    36061 ggatccgttt tgtcatagag cacatccacg atgtggtgca tgtcccgcat gccgacgatg
    36121 aactcgccgg aataatccag tccgtacttc cccagaatac gtttggtcat ggccatctgc
    36181 ttgagcgtct cgctcccgcg cgcctgcgag accggtgcga accacatgga gccgccgccg
    36241 cgccagttgt agagcgagaa ttccttgagg gtcatgtcgc cgcgcatcaa tgccgcgcga
    36301 tactggaagg ccggatcgtc gcccgcctct tcctccgtga ggattttcgc cttgccggat
    36361 ttgctgaacg cgtccgtcac gatcttccaa ttcacgtcga tctgttcctg cgtgccgtac
    36421 agcgccgcgt agacgttcca gactcccagc ttgtggtcct tcatgatctg ccgcaccgct
    36481 tcgtcagtga tcgccccggg cccggtcaca tagtcgcttc gcttggcctt cgtcggcgct
    36541 tcatagagcg catgggcgat cacgatcgca ttggggatga cgccgctgat gcgaagcggt
    36601 ctgatggtct cgacgatttc cacgatatcg tcttcgttct cgtactgaat gacgaacggc
    36661 ttgaaggccg gaggggcggg catcagccag aatccgaact tagtgacgat gccataattg
    36721 gattgggtga agatcccgtc gaggtacgga ccgtaacccc acttgaagac ctgccaggta
    36781 ttggacttcg gtaacgagcc catcccggtg cgcagcacct caccattggc cagaaccacc
    36841 tccataccgc aggcaaagag gaaatgttct ccgtagggcg tatacccgac gccgcgatcc
    36901 aacgtattgc cggtcggtcc ggcaatggcg gaaggggcgg gacaggagag ccacagcggg
    36961 tacttatgtt cctggatgta atcgtagagc tgtttgtagg tgatgccggg ctcgacgagg
    37021 gcgaaacaaa gatcctgatc gacttcaagg atgcggttca tccgtttcag atcgagcacc
    37081 acctggcccc gctcggccgg tgcggcagaa ccataaccga agttccgacc ggtcgagatc
    37141 atccagatcg gtactttgta tgtattgcaa atgccgacga tcttctggat ttgctcaacc
    37201 gtcgtagcca ggagcgcggc ggacggagta tggtccgcat cgggaaccgg catcatggtc
    37261 ttgatgtaag gggcgagctg ttccgccgtc acgaggacgg agtcttgacc tactatacga
    37321 cggaattccg cgatcgccgc cgcaaaggac ttctcggaga cgcttttcgg taacgcgata
    37381 aatttgctgg ccatgctgtt cctcctgtcc gcctaaatat ccatcacgaa tgagacgaat
    37441 cgttgtgtgg gtggcaacga atggggattg aatgcccgat ggacgaacgc gcggcttgag
    37501 caggactccg gaatcgaatg gatcccgaga gccgacgccg ccacggcata acccacggat
    37561 tccacccact gtggagactg ccgcgagacc acctcgcgtt gggagacgtg cgatgggatg
    37621 ggcgtccatc cctcaactcc ggccaggccc aactgtgcac atccttccaa gagcgatagt
    37681 cccgaacagt ggagatgaat cgcgtcggac ccggcagacc gataggagga aaatcccgat
    37741 gcagaccaat tggtcaactt gctcgcggcc gatgatccgt cgaggaagct ttctgtaatc
    37801 gaaaaacttt cacccgcacc gatcagtcgg gtagccagga caccacccgc gccctgagca
    37861 gatgaggccg caagccacgt atgtcgaagc ggacaggcgt catccttcgc agaggcatga
    37921 gaaccgaccg acagcagccg gcctccggct gtccgagcga gttcctgaaa gaccgccgca
    37981 ctcgcatcgt ccatgacggc gacccagcgc gcgcctttcg tctgttcgag caatgacacc
    38041 agcttgccgg ggtcggacag gagcccgccg tctacctgtc gcgtctccag cccgtcgtat
    38101 cccacttcct gacaggccgc gcggacaccg ttcgcaaacc gttcgtctac gtagttgtct
    38161 cccaggaaga gcagacaccg cttggcggtt ctgttcggtt cctccgcaaa cgtccaggca
    38221 ggtgtcccga gtgccagcag ggctcctccc gccaatagac ctttcatgag gttgcgtcga
    38281 tcaatatcca tggcgtgagc cctccctact ggttggctgc agcgttcgaa acatactcgg
    38341 ccaccttcgc cagcgactcg tcgtcgattt ccgccgcgcg gaatgacggc atggcccggt
    38401 tgccgtgtcg gacgatgaag gtgatgtagg cgggagcaag gccacggccg cgaatggttg
    38461 ggccgacctt ggcctcatga caaaacgcgc agaccttcgc acagacttcg gccccgtctt
    38521 tccaccgaaa cccagactcg ggagtctctt gagatgtggc ccctgcgatg cgtaccacat
    38581 tcccgagcaa cacgaatgct gtcaggatca aggccggcac ggtccatact gcttttaacg
    38641 cttgcatggc atctcctccc tatccaatcc gacctctact tttccgcaag ccacggggtg
    38701 ctcccccgaa cttcttggta gcaatcgtgc tgcatatcct aagccaatca acaggcgacg
    38761 gcgggaagat cgcgcaaagg cccatggccg attcgatgaa tggcccgtgc ctcagcggca
    38821 cctggcgtgc acgtcacagt tcagaccggg acgatccaga acgaaacgct ctttggggcg
    38881 gcggatcggc aggcggggac atcgttgtct gcgtttcaga ggcaggcaga caacgacggg
    38941 atgaaagctt cctgagtcag tagcccgcga atttttcagt gcgaatgtcg ctgtcgtcga
    39001 tgccggcgcc tttcaacatc gtgcgcaacg cgccgaccat acccggcggg ccgaccacgt
    39061 agtagatcgg cttctccatg ttcacgagat gtttggagag cagagccgcg ttgagatacc
    39121 ccgtctcgcc ctgccaggga cgggaagatt tcgttggttc ggtcatggtg cccacaaagc
    39181 gatagtgagg attcttgtcc tgcaaggctt gcagctcatc caggaacggc gcatcctccg
    39241 gtcgccggtt ggagtagaag agcaccatgg ggtgcggcga ccgttgcatc gccgcctgca
    39301 ccgccatgct gcggaagggt gtgatcccga tcccgccggc cagaaacacg gcggggcggg
    39361 tctggtcggc atgcagcacc aattttccga acggcccttc catccggacg gttgagccga
    39421 gcggcatacg ctgcagttcg cgcttgaagg cggtatcgcg gagccgggtg gccaccatca
    39481 gggtcgattc ctgcggtgcg ctcgcgatgg aaaaggccct ggtgttgccc gccgcatcgg
    39541 tctcagaggg ctggggcagc gtcaggtcga caaactgacc cggcgtgaag gcaaactgcg
    39601 tcggcttgtc gaagtagaac gccatcgtcc cttccgccac cagccgccgc tctgtcagcg
    39661 tgatcgaata cccgttctcg ctcgccgcct cacccatgat tcacctcttg tgtcacacgc
    39721 tgcacgccgg tcaaccggga actcagagcg atttgtgcgt cccgtcgcaa aacggaggat
    39781 tcttggtgtg cttacactgg cacaaggcca cggttttctt ttcggttgtc gtgaactcca
    39841 tgggcgtgaa actggttccc ttgtgagacc catcacaaaa cggttgcttc ttcgagcgcc
    39901 cgcaccggca ccaatagtac gtgcctgctt ccaactccaa cacggccggc tgcttcgctg
    39961 caatgactgg ttcttccatg ctctaccctc ctgtttaacc gaatgatttc acttccgcac
    40021 acattcttgc cccgccgggc tccgatcact tacacgcatc agaggcaaat ctcctgccat
    40081 ctccagcagg cgaagggctc gctctgccac ttgccggcac aacgagtatc ggcaccgttg
    40141 agaagaaatg cgccagcttc cacctgcgtc ctgccgcaga aagggacaca ggtgatccag
    40201 cccggcaggt cttggccagg cctagcaagc tgcggaaaca ctcgattgtt gcgcaagacg
    40261 ttatgatcaa ctcgtgcgcc gtttcggtat cagaatcacc cgcaggatgc gcaaaatggc
    40321 cgtccagcaa ggccgcagcg agcgaagagg cgaatcgtac tcgtgcggta cggtgagccg
    40381 cgaagcgatg cgagaacgcc gctggcggtc tttttccgca tcctgctaga gcgacagcgg
    40441 cttgatgggc gcaaggacgg ccgacggatt gtttttccac aaggcttcgt ccgcgtccac
    40501 gtacagctcg acctcattgc cgtccggatc ccgcaagtac aaactctgac tcaccgtgtg
    40561 gtcgctcatc ccgtcgatgg agacgccggc ggattccaac tcccgcttcg ccgccctcag
    40621 ctcatcgagg ctgtcgccga ctttgatgcc gatatgatag aggcctcgac gtcgcccgga
    40681 tggaggaccc ggcgcatcgc ccacttgaat cagcagcagc tcatggtgcg tgcggccgga
    40741 tgtgagcgcc gccgcagcgc cattgaaaat tctccccact tcctggaatc caagcagatc
    40801 gcgataaaat gtcagcgacc gtccaagatc tttcacgtag aacaccacat ggccgaggta
    40861 gtgcgctttc atgtgatcga ctccttcccg ggcgacgatg tccggagcag cgccctgact
    40921 gccgcatgcg ccagcaccac atccggctcg cgctgcaacg cgtcgtaata atgccggccg
    40981 aatccattcc cttcttcgga cggcgtcaac atggtccgca ccgagtcgat tgccagggtc
    41041 atctccgtcc ggtccccgat ctcctcttcc tcggacagct catcgatctt caccatcaat
    41101 tgagacagcg ggcgcaacca ggcgaaccac gcgtcaccca atacaagctg aaagaacgca
    41161 ccattcgtct cgatccgtcc atgcacccgc tcataggaga cccgttcgct gtccagcaac
    41221 gccttgtgca gacgcagcaa tgaccgcgac agattgccca ggtgcgcgcg catgtgatcc
    41281 tcacctgcca tgatcttgcc tccttgcgaa tccgccccgc ttatcccgtc ggttcactat
    41341 ccagttggtg cgccttgcgg ccaccccggg tgagcggatg catcgccagc tgcccctcca
    41401 ccatgagccg cgccatctcc gccgcgcggg cgacgagcga ctgagccccc tcacgcaact
    41461 cccggctgtt ctgcacctcg ctgttgttcc gctccatgtc ctctgcgctc catccgcgcg
    41521 aatgggcgat aaacggaaac tgcgggaact ggcaaccgac ttccgcaaag aaggtcatca
    41581 gttgaccggc gacaccctgg acattgtcct gtccacccat gatgatgaac gcggcgactt
    41641 tatttttgag caggtgccgg ttggcgatcg tctcctgatt ctgaatgcag ttcatccgtt
    41701 cgaccatttt gtaatagaga ctgctggctc cgccccatcg gatcggggtg gataccagaa
    41761 tcacatccgc ccaatgcacc acggcttcgt acacccggtc catttgatcg gtcggatcca
    41821 tctgcgtaat cgaacagggc caggtgcagg cttccgccga cttggaataa aacccttcac
    41881 aggcccggaa cgagaggtcc cgtagcttga tcagttgcgt gtcgagctgc aggtgttcgc
    41941 gcgcatacgc gagtgcctcc tccagcaggg catcggaggc gctgaagcgc ggctggtccg
    42001 ccgtcatcgc cgtcgtggcg atgcccagta cacgaatggg accgtcggct cgaacgatcg
    42061 ggcgcgccag cgggtgcgat ggtttcggga gcttctgtct cttcgtgacc ggagtcagat
    42121 cgacaaagac gcgtccatcc tctaccttca cggcataggt cgcaacctga tcaccttcat
    42181 atccaggctc gccccggccg gtgcgatggt gaaacttcca gtaatgccag ggacagacga
    42241 cgtagtcgcc gtcgagccgt ccctcaccca gcggcccgcc gatatgattg cagacccctg
    42301 aaatcgccgc gaaccggccg tccttataac tcaatgcgag cgttgtctgg ccgcagacga
    42361 tctgctggag ggggcgctgc ttgagttcat cgaccgaacc gatctcagtc catgtcgcat
    42421 ccggcatgtc gagttccttc aggcgtcagc cacacatccg tttgtcacaa gcgcttctct
    42481 tccagatcgt acacatcgaa atacacgtag acggggcggt gactcggcac atatttcggg
    42541 atgtagtctt ccatataggc ttgcacctgc ggggccatct cggaaagacg caggtcccgc
    42601 atcttgttgt tccactgaat gattgcctgc tggtcctgta ccttgctctg agccttgagc
    42661 caggcggcca gttcctcatc gttcgccccg gtggctacga cctccttgaa ttgctccggc
    42721 gtaatgcccg tgaacgagaa caacaggccg gccaacgagc agggccaata gttatagtcc
    42781 ccgttgatgc ccaggagaaa cgcccggcat ttatccgcgc aacgcgcgcc gatgacataa
    42841 cctcccagca tttcccgcgg gctgcgagga taggtcttgg tgagatcttt cgcaagcgtt
    42901 cggaccttct ctagatcagg cattctgtca ctcccttcct tgtcgaacac gcgagggctt
    42961 ggagaatgtg attcccgcct tctggacctc ctgatagggg aggagccaga ccagggactc
    43021 gtccgataga tcaccgacct gaatgcggtt cccccagggg tcacggaaat cgcagcggaa
    43081 atgcggctcc atcctcagct tgtacttcct ggtgagcttg cgccggactt ctttgatctg
    43141 ctccgcatca cggaccatca acccgaaatg cttcacgcga tccggctgca gttgctcgac
    43201 ttcaaaaatc gccatgaatt gatgttcgcc cagtttgcac caggccgcgc cttctccacc
    43261 gcgcaacatc ttgagtccga acacgtctga ataaaacttc acggccttct tggcgtcggt
    43321 gacttctatg acgatgtggt tgcatccata cacttgaacc ggcatcgcag gacctcctta
    43381 ccccgtaacc tgtatgatcg cagagacgaa cgtctccacc ggctgggcac cggagagcgc
    43441 agcctttcca ttcagcacga aatagggaac ccctctgatg cccagctgat ggccgtgggc
    43501 ctcctcctgc cgcacggcat cgacgccctc ctgagcgtgc agcagacgga cgactgcttc
    43561 tccatccaat ccggcccgga cggcgaggga gaccagtatc tcaggttgcc cgatattcag
    43621 tccctccgtg aagtacccgt ggaacagttc ttcaaccacc tcatcctgac ggccctgttg
    43681 ctgagcgaac cagatcagtc gatgggcatc gaacgtgttc ggtgttcgcg caatccggtc
    43741 gaacacgaac tcgatgccca cactcgttcc cactgctgcc acgcgatcct ggatcgcctt
    43801 catttcaccg ggcccgccga acttcgcttc gagatacacc cggcgatcca tgccggcctt
    43861 gggcatggtc ggattgagct gaaacgggcg ccagaatacg cgcgccgtcg cctgcacctg
    43921 gaccgattcc agcgcctgtt ccagccgccg tttgccgatg taacaccagg ggcacaccac
    43981 gtccgaatac acatcgaccg tgagcgcctg accggtcatg acaaatgccc catctttccc
    44041 gcctggtagt catgcacggc ctgaataatt tcgtcgcgcg tgttcatcac gaacggaccg
    44101 taccgggcga tcggctcggc aatcggttcg ccgctcagca cgaggatggt tgcatcctgc
    44161 ttcgcctcca caacgacccg ctcccctcgc tgaccgaaca gcgcgatttc cacttcctgt
    44221 accgtctgcg acccgttcat cacgacctct ccgtgcagca cgaacaggga cgaattatat
    44281 ccctcgggca agttgagttc agtccggtgg cccgccttca accgcaggtc gtagaggtgg
    44341 accggactga aggtctttgc cggacccttc accgcgcgaa actctccggc gatgacacgt
    44401 acccggccga ctcctccatc gagctcgacc gcaggaatct ccgcatcgac cagcgtctga
    44461 tatcgtggcg cggtcatctt gaacgccttc ggcagattga cccacagctg aatcccctcc
    44521 aacaggccgc cctgcttcgc gaattccttc tcatgcaact cttcgtgcac gacacccgag
    44581 gccgccgtca tccactgcac gtcaccgggg ccgataacgc cgccgctgcc tgtcgaatca
    44641 cgatgcgcca ccttgccgtg atacatgatg gtgaccgtct caaagcctcg atggggatgc
    44701 tcgccgaccc cgagctgccg gtcggtcggc gggaactgct ggggccccaa ataatcgagc
    44761 attaaaaagg gcgacagctg ctcctcgact ccggcgccgg gaatcatgtt gcggacggga
    44821 aacccgtccc cgaccatatg atgggaccca gcctgataca ctccgacaag gtctttcgta
    44881 acggatgtgt ctgttcgcat agtgcctcct tccttacgcc attccgcgca atgcctccgc
    44941 gaggggaatg gtccctcgca ccagcgaggc ggggtgttca tacccgatcg cacctcttga
    45001 gaacgcggtg tacatttcct cgaacaagcc ggctgcttct ctggaaaagc cgaacgaggt
    45061 caacgtcggc accactgcac tgagcggcgc cggttgcgtg gcgaccgccc gcccaagaat
    45121 ccggccgagc gcctcggcca cctgttccgg actgtactcc tccgggcccg caagctccac
    45181 caccgtatgg ccccttccgc cggccatcaa tcgctccgct gcgacgcgac cgatatccct
    45241 ggtcgcaatc atcggcacct ttgcctgcgg cgccatgaac gtgggcagca gaccctggcc
    45301 ctttgccatc ccgatacccg gcgcccagtt ttccatgaag taacaggggc gcaagatggt
    45361 cagattacga gccaccggac tcaatttttg ctcaccgtat cggacggcac gtatagggcc
    45421 ggtgccctcc ggtatctggc cgccgacgga cgacaggaac acgacgtgcg aaatcccgct
    45481 ggctttcacg gcttccgccg cgcggtccaa ccgctgccgc tgctcggcga accaggccgt
    45541 cgccccatag ttcggcggaa ccagcaggta gacacccaag gccccttgca aggccttcgt
    45601 caaggcgggc acatcatcca acgaggcgac ggccacctcg gccccctgtg atttccaggc
    45661 tgcccccttg tcggccgaac gaaccaccac acgcaccgac tgtttgcgag cgagcagcgt
    45721 ctctgcgacc acggctcccg tatgtcctgt agctcccaat acgacgaaca tctttcggtc
    45781 tcctttcctg cctcaggcga ctcgttccgc cacgatccga tcaatcgacc atcgcccggc
    45841 tcccgtcacc agcaacgcga cggccagccc gatcaccagg agatggtact catagccctc
    45901 tccctgctgt tgcccgaacc aattcatgaa aaacccgacc ggcagatgca ccgtcaaaat
    45961 ggctcccagc atgatcacaa tgaagctggc ggcggtgaat cgcgtcaaca ggccggcgag
    46021 caaccccagg cttccgaaaa actcccccat gataaccaga aacgcgatga tccagggtag
    46081 ccccattttc tgggtgaaaa aacccatggt cccgtcgaac ccgaatccgc cgaaccaacc
    46141 cagcaacttt tgagccccgt gcggcaacat caccaacccc aaggtcagcc gcaaaatcag
    46201 gccggaccaa ccgtcgtctg tattgaatag tgctttcatt ttcgtaccct cccgtgatcg
    46261 accattaacc gtctgactct gcccaccgct cagatcgccc ggcgaacagc cgcgcgggcc
    46321 gacgccagta taatagttct aattagaact aagtcaagat caaatattcc tcctcagccg
    46381 gaaggacacc tattcttaca gatcagtcag ttgtggttat agggacatac gcgacgggtc
    46441 acaggcctct caacgacctg ccgggacggg ggattgcatc gaaaactctg gggaacaacc
    46501 gagagctcgt ggactctctc gggaaaggag ggagggagga agagaggtgt gagggacgaa
    46561 gctgcttatt tcctttgctt gatcgcgcgc tcgacttcgc ggccggcttc acgggctttg
    46621 atcgattcgc gacgatcgta ttgtttcttg cccttcgcca aggctaactc cactttggcg
    46681 tggccgcgct tcgaaaagta gatgcgcaag gggacgagcg tgaggccctt cagctgggtt
    46741 ttcccgatca gtttgttgat ttctttgcga tgcaacagca gctttcgctt gcggagcggg
    46801 tcgtgattca tcagattgcc gtgcgagtag gggctgatgt gacaatgctg caagtagacc
    46861 tcgccgtcat ccaccgaggc ataactatcc tggaggttca cgcgaccttc ccgcaacgac
    46921 ttcacctcag tccccctcag gatgaccccg gcttcgaact tctcttcgat gaagtagtcg
    46981 tgatacgcct tgcgattggt ggcgacgact ttgtactggt cttctttgtc gtgcgttttc
    47041 gtcatctcat actagcccac atgatgcctg agcgcttctg ctgcaatcgc gggttcgcgg
    47101 ctgcgacagg cctgtcggcg ggcgggctcg ccgctcggtc ggtcgaca
//
LOCUS       DAORRQ010000034        46287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_50_length_46287_cov_3479.936992, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000034 DAORRQ010000000
VERSION     DAORRQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 46287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46287
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50_length_46287_cov_3479.936992"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK224_11910"
     CDS             complement(<1..390)
                     /locus_tag="PK224_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16487.1"
                     /translation="METTKLLHALAWYCVTFCSVGVVSSAVLAAEPPGSQTFIDHPRQ
                     GWFSYQDPSAEAEEDAEESPVPVTMDSFPPEAWLEPSKYRTLFKGLSVEGVKLNALPM
                     TVLRELVSAKKERALDHPSVENVTTYIK"
     gene            complement(360..1448)
                     /locus_tag="PK224_11915"
     CDS             complement(360..1448)
                     /locus_tag="PK224_11915"
                     /inference="COORDINATES: protein motif:HMM:NF018536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HRB16488.1"
                     /translation="MNKLSKRRIQHMLLLVCLSCVLTGVATLWPSGSAEAVCTTGTNV
                     LANSLATTCWECMFPISLSGVTVFNLGEDFTEPAIGPGHAPFPPQLCGCVCLGPICIP
                     GLPLGIWEPKNLVEAVHDPLCFPTLGVSLAGGFSYIGRGMATTQMDSELNYAFFHVHY
                     FVFPLWAIVGIGMDILCLNPTGIADEIDLAYASEIDPLWDDDLISLILFPEALVLANP
                     VALAACAADSVAAAVGFPIDPLFWCAGSFGFMYPPTGHVSGADGDLKPAALSMTRLLA
                     KLARAGTEFWTSANGPLLCEDIPTALIVKSQYKFQLLWPIPNRGLPPPCCQPLGRTLM
                     MWGLGKIVPAVGEDFVWLLWKRQNCCML"
     gene            complement(1441..2517)
                     /locus_tag="PK224_11920"
     CDS             complement(1441..2517)
                     /locus_tag="PK224_11920"
                     /inference="COORDINATES: protein motif:HMM:NF021209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC family F-type conjugative pilus assembly
                     protein"
                     /protein_id="HRB16489.1"
                     /translation="MAQDIARYSRQALRHGRPRESPKEVRRDGVYLLLSFSLPNDTLK
                     DYLREAKLLGAKVLLRGLVQDSFKVTQERIKHLLFTGDHPDDSLLVGIGIDPVIYRTV
                     GAGEVPALVFVKDEKFLVASGGASVAHLLTLLSKEQAGVMPWIEWFERRHRGFLQGGP
                     TEEAPPQVPTIGQSVKVQGDARGADIAERDMIDVMKERVAKADWPDIQRRSGDALKRQ
                     FAKGPGLALPHAEEARVVLIDPTVEYPEDIKDPTTNTVLIKSGTKINPFDKVRWIRTL
                     VFFNGTSSSQVGWVQQYLKEHDPKFVKLIISDGDVQKVMEQLHQRVYWANPLLVSRMG
                     VGAVPSIVSQLGRHLRVEEVAIHE"
     gene            complement(2692..3270)
                     /gene="lepB"
                     /locus_tag="PK224_11925"
     CDS             complement(2692..3270)
                     /gene="lepB"
                     /locus_tag="PK224_11925"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRB16490.1"
                     /translation="MKLFNALSLLNVLKRAPTILDWLDRHRWTHVGLMLFCGAIITAG
                     VIAPWYEVAARMSGSWPHHNFFLIQKGQPVGRGDLVAFIMRRDYVERVQPGHLHRDYA
                     DVGNRWMKRIVGVPGDHIVIKEDEVYINGQSVGRGFGIDRYGQWIDLASLPEVIPEGE
                     YYVALPHPRSFDSRYYGLIRKADILGTVTPLI"
     gene            complement(3200..5713)
                     /gene="traC"
                     /locus_tag="PK224_11930"
     CDS             complement(3200..5713)
                     /gene="traC"
                     /locus_tag="PK224_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HRB16491.1"
                     /translation="MSTILEWKVKQLVDRTAISDWLPYEAWDDTNQVYRLDDGRIGVV
                     FSSKPLLGLSEESIQKLTNLFESDLPIGTTFQFMLHASPIIEPYLDNHVILAQRGQAG
                     ERYVADARKTAEFYLHARDTQLTSNPTLRPRDFTVYVSVAFPTQETTAQGWEDERIHQ
                     AVSGIEGQLNTLGLLPERVGPLQLLSLLHVLLNPGHSWESRPPAYSDQLPIRDQAVRL
                     DTKATLRTKMIELDGKFLKTLTVQSWPVQWHGGNNRELIGSLIRFTDQITCPFFLTLN
                     VLKFDQVKAKGKLRKKHVIINQQASGFLLGLIPSLKFKLDHFNGVQAQLENGRQLIGS
                     YFQVVLYGDTPRHVEEQTGALRALYRAKDINLQEDHFIGWKILMTGLPLGLPSDDKAL
                     IDGLRRMKTYHTEVPAHICPIVADWKGGGAPVVLLTSRSGQLITMDVFANQQTNYNMA
                     IAATSGAGKSFFMNNWIKSYLGIGGQVWVIDAGFSYVKLVELLKGQYIQYGANAQRLC
                     FNPFSKLVHWGSNAGGVDDPSDRADELATLKALHAQMASPSRPLSDVELSFIEEAIMR
                     VLEGEGSEGCPDSVHKVLKSMTDPRARDLARVLASYAKGGMYSHLFNGANNVNFENDF
                     VVLELDGLNEQKQLRSVILLQMQMNIQAVMYKKENRGRRKFVILDEAWDLLSQGGNTA
                     AFFETGARRVRKSGGSMITITQGINDYYDKMRDVGRYLLENAEFVGLLKQKTESIAAF
                     RNNGRIVLSEMEYRLLSSVTKTTEYSEIFMITPFGRGICRLTVPRETQLLFTTNPDEL
                     SMIDRVRKEGPKELSIDEAIQCIIAAERAQAGADNPRLA"
     gene            complement(5738..6469)
                     /locus_tag="PK224_11935"
     CDS             complement(5738..6469)
                     /locus_tag="PK224_11935"
                     /inference="COORDINATES: protein motif:HMM:NF021212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HRB16492.1"
                     /translation="MMSGYIRRYITRGGSWYGLGSGVAMGLSVLLAGCGGYESNFSCK
                     GYPDQATCESVSEAYEKRFSAGQQVTQYTKDQKGGGQDASSSSGPGQVTPPMIFSGKP
                     DSAIGKPVITPVSALRVTVFPWKDTKKRLHDQSRHYLIVDEPDFIFGHMAQGVSAGSS
                     SGHGKDLYPRMGRMVEKRDGKRTPSGNAAANAMTENVPEVGQRSAPMSVNPLGSGGIM
                     PQGFPQLPQTGASNYLTDDGPLPPQ"
     gene            complement(6508..7284)
                     /locus_tag="PK224_11940"
     CDS             complement(6508..7284)
                     /locus_tag="PK224_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HRB16493.1"
                     /translation="MRRSYQQIIAGLVCAVCVSVAGESILRAETDDAVRSIKTVIEQK
                     FPGMKVLRVSKTEIHGWLLVESDSDRVENYMYVHESGRYVLSGALFDIQMGRNVTQEY
                     VRDRQQALLAGMDTSKTILLSPMQSKLERPVLVFDDPDCRFCQQFHPEVQKLVEAGVP
                     VAVVLYPLVRTHPDAYRKSVAIWCAPDQAEALGRALMSKPVMGEVGACSHPIDENIKL
                     GKRLGVNSTPMVFLPNGQSFAGYRPASEVLALLQIEAQQK"
     gene            complement(7314..8600)
                     /locus_tag="PK224_11945"
     CDS             complement(7314..8600)
                     /locus_tag="PK224_11945"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HRB16494.1"
                     /translation="MASIKPTESAILNPGDPIRPEKPAPSKLLAGVVLVGGFIVLSMV
                     LQQFRGNQPGIVRGKSPAKTVSINREETDKEIYMKDAGARIEALEQQRDQMRRLLEQD
                     GRDKTEIKTQLSDLQKRIESVARSGGVVSAGTKSGALPPLPPPLSANPVGGGSATPVS
                     AMPVPKGPIPVQSTPKPAGGTLPAPGAGGGLTDGFQVFRPEIVPGAKKNEKKLWLNTG
                     TIIPVKLLSGMDAPARSTGAQGGGASANSPYPVLMMVRDIMKLPNEARIDAKDCFLVG
                     EGLGDLSSERVMIRGNALSCVKKNGEAVDIELKGFVSGEDGKQGMRGRVVLKEGALLG
                     RTLLAGFVSGISRAFMPYQSGFFVAQSPSQAFNLPPAGPVAMSGMAGGAGRAAELLAR
                     HYSMMAASIFPVIEIDAQREVDFIVGTGRELEGFFQ"
     gene            complement(8621..9394)
                     /locus_tag="PK224_11950"
     CDS             complement(8621..9394)
                     /locus_tag="PK224_11950"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HRB16495.1"
                     /translation="MTSSVYRDSKHRAMAMCVLGLLLFLSWGKSVGAAVVVGDEGATS
                     LTLSGRDLNVLTFPAPITMATSSRADLKAKPEGRNLIVQVKTGPADLVVMAGEQTYVF
                     EVGVSVSLGAQTISIEDRRVGTVVYDTDPARQAADYVDGLLETLQMAARGALPKGYRQ
                     TSIAEERYPKWLELKVVQGIEYRGPKYRVIVYRLVNTAGHKYILREQEFLTGVQKAIA
                     LNREVVESGEEVVVYLLDDAPPEPPKPKPEPKVDPDAKS"
     gene            complement(9405..9986)
                     /locus_tag="PK224_11955"
     CDS             complement(9405..9986)
                     /locus_tag="PK224_11955"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HRB16496.1"
                     /translation="MTIELWKSKLHNAQVLNLVFAALATVLAVALVANGFFRQNVAVH
                     VVPINPTQEYWANAMGGDEYYKKSIAMAMLPWIANVTPASVEFHHRQLLQWVPSSHYG
                     LMSESLGAEAVYVKTNNLSRVFFVTASRVDGDVAVFEGIERRFVGKSEIREEEREYRI
                     SMRWDQGRPWVVGLSAVHLDRSRQDSQSDPAKG"
     gene            complement(9992..10273)
                     /gene="traL"
                     /locus_tag="PK224_11960"
     CDS             complement(9992..10273)
                     /gene="traL"
                     /locus_tag="PK224_11960"
                     /inference="COORDINATES: protein motif:HMM:NF018836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HRB16497.1"
                     /translation="MNGTFIPRYLDRLPQIFWWEIDELAVLFVCIFLGIVTKFLTYLI
                     VVGIVSTLILSKMKNGKSEGFLFHWAYWSGVPTFQLTGSPHGTTREMME"
     gene            complement(10352..10672)
                     /locus_tag="PK224_11965"
     CDS             complement(10352..10672)
                     /locus_tag="PK224_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16498.1"
                     /translation="MGNVLKRVTGEMWRMSLLLGLMVLASFVVLAVPAFAGADATFGP
                     LVTLLTNYMQGSLGTLLALVAGAIGLGSAMAGRWAGMFTGFGVAAAAFYIPNIIPTIT
                     TGLL"
     gene            complement(12005..12322)
                     /locus_tag="PK224_11970"
     CDS             complement(12005..12322)
                     /locus_tag="PK224_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16499.1"
                     /translation="MQAHRTRQQDIGKERSTDLEAEGFVGACTQSAEFQEMAVRTIVR
                     RLDVLCEALEGKAQQRGVELEAPRESQEAIARIASRMATLGTLIHAPVMHAVIPDNNT
                     SVL"
     gene            complement(12502..12792)
                     /locus_tag="PK224_11975"
     CDS             complement(12502..12792)
                     /locus_tag="PK224_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16500.1"
                     /translation="MPSSAVGGLMLEYAADWQESLVQRAEQCARSITYVAGELIRERF
                     RLGGIVVEALERSQHGDAVVVKMAECMSAAVGKCIRPHMLYESARIYNAFGG"
     gene            complement(12782..13594)
                     /locus_tag="PK224_11980"
     CDS             complement(12782..13594)
                     /locus_tag="PK224_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16501.1"
                     /translation="MRGGRKWLLAVAGALGLVCLGGNVVMALSPANISPGYPTSLDDA
                     YPASTDQVVIQSAIRPDITRNNSGSESGRFRATHDIRYGATENMELTVSGTSLRGPLN
                     PGTMDDPRSIQLGILARFRKQAEERELLPSISVRVLGEVPYAGDRTNPGLRGALVTSW
                     NLGNRWWLHGNLGYEVVPSFQPGHWVPHRSSIILVNLGFVKALDESSAIVGGLRFQED
                     PLNSKKQVASPELGYVLALDKHWLFSLSASRDFLGSVGQAAFRGSVGVIYAF"
     gene            13957..15363
                     /locus_tag="PK224_11985"
     CDS             13957..15363
                     /locus_tag="PK224_11985"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRB16502.1"
                     /translation="MKRIQAYKFELMPDGQQQNHMRRFAGSCRFVFNRALALQKERYE
                     QREKNLGYAGLCKLLTEWRNSQETAWLADAPVHPLQQSLKNLERAYANFISQRAACPR
                     FKKKGQSERFRYPDTKQIKLEQGNSRLFLPKLGWLRYRNSREVLGTVKNVTVSQLCGK
                     WFVSIQTEREVEPPLPNGNAVGIDMGIARFATFSNGSYLAPLNSFTQHETRLRKAQRA
                     MSRKTRFSNNWKKAKARIQRIHARIGNVRRDYLHKATTAISHNHALVIVEDLQVINMS
                     ASAGASLEASGRRVRAKSGLNKRILDQGWFEFRRQLQCKLAWNGGWLLAVPPRKTSRT
                     CPDCGHVSRHNRQTQAEFRCVECGFEGHADVVGAINILRAGHAQLACGEDVSPATPAK
                     VKRAASAKQEPTEATLARFDHSPERRKNLRSSGRGGCQNPKSFAHMPDRILHPREIPC
                     DKIARHILHNRITLPLNR"
     gene            complement(15649..17436)
                     /locus_tag="PK224_11990"
     CDS             complement(15649..17436)
                     /locus_tag="PK224_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRB16503.1"
                     /translation="MVCSGSLEVHVQGEVVSAEMAGSPEEMPKVVGVITSRTRARRGS
                     GDWIEIARKHRAFIPDGLSVPSVGEVGECWGRVIANGPDPFLMVSRIRSRRFEACASS
                     SELLGVLGLRRSPPRQVVKVFQLLGPLVEWLCCAGWKAVARKLVKGTLRSVQAIAADP
                     FLLYRRRDLPFQAAVAAAQVLGHDLYSDEHYKAAVMEALAQADEEGIGGISDAHLVAR
                     CAELIGLPADRVAPSITLQAGRAGKCEQGLWFSPGVYFVRQKALAMIRANQQDSGNAS
                     PSVQARALRYRYTVVTGGAGSGKTELARSLSAQVRERGGRVAATAMTGKAATLLGEDA
                     TTLHKLLGYGGGGYSVSSVGADLVLVDEAGMLTWHILYRLLLSCRGQIVLIGDPAQLA
                     PVGATPVMAELLTVLPVVHLGEEGSKGSLLVKVQVIRFASEALLLHQLRKVVCGYQDT
                     GVEWQALSPVYAGGLGVDRLNRLLQEIVNPDGLPCHGGFRTGDRVIVTKTRYDGGQRA
                     VNGEQGRVLGGMGDTIALRLDSGREVALRADELQLSYCITVHTAQGSRYQCVVFIISE
                     RECGAFAVEERMQYVGRTRGREATVCMVY"
     gene            17596..18732
                     /locus_tag="PK224_11995"
     CDS             17596..18732
                     /locus_tag="PK224_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRB16504.1"
                     /translation="MLAELIHPLALMEHQEWEYRPRPSNAGPDHCIRKLTYHARHAPA
                     KSPSGRFLVVLDDSTWHEELVLNWIAKSTFHLHSQQLELTCATTIFQGQPFPIKGHID
                     GVITDLLGIDRLLELKAVEHFTFTRYAEGNYPTNYLVQVVFYLVGLAALNPHIDEALL
                     LIKNKNQSAFLEYRVRYDRDNDRLTVLEITHSTGTRVYPHQDFVGLYQEALRRFEEVE
                     EYRDQDALPPRPYPNADNFHCAYCPYQFCWEGVDQVPIEGIAELRTGLVPAVEEYLDL
                     QTELAALTPKQKRLDALKQQFKLELRTEGITRAEGHGYYIASSPYEQSKLDQTKLSKA
                     TIERATVKETYSRFSAGRLSKVVDATKPKRRSPLKKPKPTTQIA"
     gene            18771..20105
                     /locus_tag="PK224_12000"
     CDS             18771..20105
                     /locus_tag="PK224_12000"
                     /inference="COORDINATES: protein motif:HMM:NF039495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16505.1"
                     /translation="MSDADKPTFIPMNRINGISNRRRMPIIGKIRLGVKVSGPSGFYP
                     KETPHFVVPEEVAKVFGAQPKELEVMFVSNDPTVNCPQALRWYGESAGLQCIGNNQVA
                     RRLNQDTWKTESRSCPCEKLESGECGPRANLIFMIPKVSSGGLFQIDSSSINSMEKIN
                     SYFDFLALTLGRISMIPLKLRRVPVTRRREGRPTTHYPLELRYEGSADETTKHQQETE
                     TVLRKIKMLEIQEPIEINPVLDPGAHVVMEEELEEEPSPVPAESVGEPGSETHNPAIP
                     PMSMPETAAAKAPEATPLPAGPPISLAQTNYLLRLTKDLPLTEDEVRVKVRALTQREA
                     SGLIKKLLGEDFSFFTQSSIAPPATPVNTGSAADTMTDPQFKKIMGLVLSMDIPEPHV
                     KSLTAGYTKRQASTLIDQLVGGDFSAFQEIKEEELPVLTPPKPTPVEERVGF"
     gene            complement(20250..21641)
                     /locus_tag="PK224_12005"
     CDS             complement(20250..21641)
                     /locus_tag="PK224_12005"
                     /inference="COORDINATES: protein motif:HMM:NF022473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2610 domain-containing protein"
                     /protein_id="HRB16506.1"
                     /translation="MRKNSYGRIVWAIMGGLILLPPQVSVAQTAQATSECDRLAASVF
                     DSTKPIEITGVRYETIDFEKAIPACEAAVKTQPNNARILYQYGRALDAGKRESLAIET
                     YKKASDLGSLLAANNVGTLLSRGYSGAKPDNKAALVWYEFAAQGGVDIAKRNAADILE
                     KGEGLAAPNLERAAMYWRSLANAGDPEAAEKVGLAILDKKVVPLDSSEVVNHLTMAAS
                     QNRFLSASRLAHLLKDRAATAEDIQQAAKYALLAYQLAFSAPSDSEEAWLPYQWTSFR
                     LYDGLVQKGAKNVMQMVAYNQALTDFPPKDGVKFTVPIECGTTKDKFDVYIWEWTRKY
                     PQTDSQADWVRQVRGCTIPQETVESFRRIYSIALENKVSFPDLAKYAFDPKNEVAVKQ
                     SSPPTQRKESLAEQSSARNQSDTNITPDETLGLAAVAVGAFACWYWPDQCKELAWEAA
                     KVGARELGKKAVQ"
     gene            complement(22005..22679)
                     /locus_tag="PK224_12010"
     CDS             complement(22005..22679)
                     /locus_tag="PK224_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB16507.1"
                     /translation="MVLVKDLLVRMRIAQNLTQTELAEKLGCTASHISIIEGGKSSGR
                     KKILPSDDMLRRIAQCLGGSPLDVQRRTYQLLLARAREVCSPEVAELLDDQILSGMPA
                     AFLERVRQDVSQYSDEDLSRVTEQLQLGGRLKQMLSGIGRLSQAEVARLAQVLGQSSE
                     TYLVDAGYLSESARLLFGRNPNCVNILQIFGSLRPETQEELLRFGEASGSLSFSDGMP
                     QAAPHE"
     gene            22945..23295
                     /locus_tag="PK224_12015"
     CDS             22945..23295
                     /locus_tag="PK224_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16508.1"
                     /translation="MNVTSPPVPEEAGKLLALIQCELNLSTKAMSNQLGITDVWMRRL
                     LNNDIIPSQHLLKAIVTLYVNHPDPCTLNHRRDLAHRFTQACARTYYERNHHRLQQRC
                     HMLFHFPELTASLI"
     gene            23318..23995
                     /locus_tag="PK224_12020"
     CDS             23318..23995
                     /locus_tag="PK224_12020"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRB16509.1"
                     /translation="MSFRTQAQQLAIDSDDTSPLLKLFQQSKPIIHSIAKRYCNLDRS
                     YEIEDLEQIGFLAVRKATEKWDFSRGNAQFQTFLYRYIQKAYQSEFDGRDKLVEITDK
                     DDQYVKTLPYSTFIKEKRRLHSQGFKSRIFRRLVPLATHEPPVSPDASPESLGLPDLP
                     NTDEVLAFTAIDRFIEFSRKRARTIPQPTALDHNLLTESEQTITRFSHDSGSSVRHAR
                     KRRNYAN"
     gene            24511..25605
                     /locus_tag="PK224_12025"
     CDS             24511..25605
                     /locus_tag="PK224_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRB16510.1"
                     /translation="MQIIMIPISQIRVHEEYATLFPPLTPTERESLKVSIQEAGIHDP
                     LRVERQPTDIASAQPYVVLAGHHRLSIAQELNIPEVPCIVVTTLELKVAALFDNIHRR
                     QLSDTLRQQMQDEEKRYRTKLRERLIPELLDVLPMLPPEVQLQLTQSNHDTQRDFLSK
                     WMAQIQQVSIQRFPEPQNNKFAAEEPDTSETPTPKGHKQLIRQINQLTKDFERQVAQQ
                     KKELEEVRQHNVSLSRQLRAQTDDKEKLHEELQSLQRQLTSDDLDHKAERILKLQRKG
                     RSQSLTIDEAGPLISGLLHTRKTIQLLQAFAQRIATLTTPDQTTILEHIKFTSQALGE
                     LEQVLPSPTASPLHTPRQTLSVHKGLSADT"
     gene            25726..26340
                     /locus_tag="PK224_12030"
     CDS             25726..26340
                     /locus_tag="PK224_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16511.1"
                     /translation="MQCSSPESFASVDHAQPGEDHRSVLRTTLSKITLYNFAAAHSLN
                     TPIELTERLLLCILLANEDRHLSRAELHQITKINPSTIDSALLNLEQRNEVHTLPQHA
                     PEQTSYELTASGHTVAISLESWTTSFYRYAECGSDAGVRDLLHQLQRAIYSLIATNTI
                     PTTRACIACDFFAPESQTGGYCRFLSTPLTIQTTRPTASDTRTP"
     gene            26415..28526
                     /locus_tag="PK224_12035"
     CDS             26415..28526
                     /locus_tag="PK224_12035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRB16512.1"
                     /translation="MSIHDANTTSVVPLPLDPSLPMEDPSEPSSHTLPSQRLPDWLHA
                     RIQTPDNITQERQITVSLDLATISPLIQNAQLNPEQLAVVTAPAEPTLVLAGAGSGKT
                     HTLIHRVAHLVTQGLPPDQILLITFTRKAAEEMIHRTQLLLQQHSLKIQGGTFHAMGL
                     IWLKHYGGALGLPPKFSIMDEDDAQGLVHLLMSKLSFSRRKGFPKKQHILALFSRAVN
                     TLRSIADTVSSDFPWLETHTPQLVVLFRAYTETKWRQHRVDYDDLLFLVYKLLCLHEP
                     TRRRLGTHYQAILVDEYQDTTKLQSEIIRLLGSHHHNVMVVGDDAQSIYGFRGAHTKN
                     MLEFQDAFPDVRLYKLEHNYRSTAPILTLANTILHDAKALYPKRLFTTKQGGQPPELI
                     ACYSEQAQSDLLTERILQCRDEGIPFHRLAVLFRSSAHSNNLELTLARHKLAFVKYGG
                     LQLTETAHFKDVLAYLRAADNPLDSNAWHRLLLLLDRVGPKLAERLIATMQEAPHPLD
                     VLDHVPGKAESTIRLLNNVIRTTQDDTISLADRLTFVIKHYEPILKRDYPEDAKVRLE
                     DLKQLCYLARQSLTLEAFLEDLTLNPSTTPGTKHAHPEHHLVLSTIHSAKGLEWDVVF
                     VIWVLDGQFPPVRSWHDEDALEEERRLLYVAITRPRNQLFLTYPSEHYHNASQQNLDS
                     LCRFLAPIPDDILPGFAMDQV"
     gene            28526..29122
                     /locus_tag="PK224_12040"
     CDS             28526..29122
                     /locus_tag="PK224_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16513.1"
                     /translation="MKSPCIFRFEFRTQCERKDRPFVARVTWDTETKSLARGFYAIQI
                     TPVGRFGAVGECTFDAAVGDIVEVKRGETKDREYRGFYLVVPKGYLILVAIADEVDDA
                     LKVVQYVAGQASPQDLAKGHWRFESAASSWKGRTAPVDQPRMEPHPPRPVAQLPPNEK
                     RQTKVTGEPASTAPRSNSTRSFTRVRRHTPQPSKDEKE"
     gene            29119..30234
                     /locus_tag="PK224_12045"
     CDS             29119..30234
                     /locus_tag="PK224_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRB16514.1"
                     /translation="MTFHIASFHVRLHYTWLIGFGLITWSLAANYFHDTQADLPVSYQ
                     WASGAIATLLILVSVFLHELAHAVTARRLGYAVKTIELHILGGWTIFERELSSPRVEI
                     LVALAGPGCSFLLTILLYALKADPLANFLYKVNLTLGAYNLFIPCLPMDGGRILRAVL
                     WTQTHSFAVATERTAQMSKQISSGMMAIGILGIIFQYQTLWLAIIGGILRMIAETAYR
                     TVQQSEQLTRPLQEVMIPRDRMVTLAHDTPLQELQDQFLRYGYRVYPTAEHGQITGLV
                     YYKDVRTDPKWLAPYGTTITPFIRNLSSDLIVHPVHTLQRALDAMLLSGSDQLLVFAG
                     GTCVGMVTRSMIARVQDAIGDPDSGPTLTRQREGFPI"
     gene            complement(30203..31612)
                     /locus_tag="PK224_12050"
     CDS             complement(30203..31612)
                     /locus_tag="PK224_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HRB16515.1"
                     /translation="MSEQGIEELRIRNRAGQRDPIVDGKVLVGYGACLQDPQRREGYL
                     VIRDEDRSGHVGIFGTTRIGKTRLMESILEQDIRSHKSVVAIDPKGDIDLFSKVVQVA
                     AEAGRLDEVMLVTPIFPDYSNYLDPLANYYMEDELVSHIVSGIKVKDDYYLSIASEVS
                     QVIVSGLIKLATSRGQVPTISFNDVNQRVGYTELINFRDVLKVLNDTEELCGLIDRVC
                     HGPGIAEHFAKVSSSLRTMLSALTFGNIGRIIGKARTNEFITRLEQGKRVILVCHTGS
                     LLSPRASLIFGRVFVSMIQSLVGRLFASGRQLDPPLCVHIDEGHNVLYPGIQEIFNKA
                     GGANVWLHFYEQSGAQMSAEIGADLARSISDNINSWIYFRVNHAETAQSVEDASPLVQ
                     RPRSVVSVQGNVTLHLNEERLVLRDRVLHLPKRCFYMRTHGQWYKGKTLDSSASYVQV
                     KFPTPAVNSPKSETPPSAA"
     gene            31772..32482
                     /locus_tag="PK224_12055"
     CDS             31772..32482
                     /locus_tag="PK224_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16516.1"
                     /translation="MEITTKMLAQPRDNQDADRHALTPEPRQLVTARGTHRNLPNRRT
                     GYTQKATIGGHKLYIRTGEYEDGTLGEIFLDMHKEDAAFRSLMNCFAIAISLGLQYGV
                     PLEEFVESFTLMRFEPNGPVSGHDQIKMASSILDYIMRDLAINYLDRQDLAHVQVTHD
                     DLRGDSVKPYAKSTNHQTRQESSSLNTMASHPTNDIEEARIKGYEGDPCPECHRFTLV
                     RRGTCRTCSTCGSSTGCS"
     gene            32527..32748
                     /locus_tag="PK224_12060"
     CDS             32527..32748
                     /locus_tag="PK224_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16517.1"
                     /translation="MSVPLPTPPHNIWLQRFKRLAQLTASLNSEDPRFPIIMTLLDKC
                     DRDYRTDNHASFIETGKRIRSLMDTPITS"
     gene            32841..34394
                     /locus_tag="PK224_12065"
     CDS             32841..34394
                     /locus_tag="PK224_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB16518.1"
                     /translation="MSEQYPLDIDFRTARNVSVPLIAVNSLDPAATMRALAWSLVNVA
                     SASQADTDNEFGLHAFPILKWDIGHGLIPLNEEGKNWHTQNVGPSKQNTTINPAEALR
                     VLEKVPQRTLTFILNGHRYLSNDFFIQGLWNCRDLYKTNGSTIVLLGIGFKLPPELAN
                     DVVLLNEPLPTATQLESIIKEQLSAASLPLPDPATLAKAVDATLGLGAFTAEQEVARS
                     MQASGLNVDKLWERKRQIIDATPGLSVWRGGSSYAQIGGVATIKHFLKQVLVSKRAPR
                     CIVWLDEIEKSLSGSTGQASDTSGVSQALLGILLSYMQDHQASGLLLVGPNGTSKSAI
                     AKATGAEANIPTIALDISGLKSSLVGSSEQQMRQALNVISAISQDKACFIATSNNISQ
                     LPPELIRRFGYGTWYVDLPSQDEREAIWNIYFAKFGLAADTDRPNDHNWTGAEIERCA
                     RLSWELSMPLSETAKYIVPTAISAKESIKALEAQAHQTYLSANREGVFDQNRDTPHHT
                     RPRTITLAQ"
     gene            34429..34746
                     /locus_tag="PK224_12070"
     CDS             34429..34746
                     /locus_tag="PK224_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16519.1"
                     /translation="MPCYLEQRVSVELNMADVPLLISAGSSLGWEHTLKDDVLVFTTP
                     QNQQVSISKGRATLDQRHEALILELKQAYAQTIIQTAAEQFGWTLERVEGAEQELLLT
                     RFS"
     gene            34768..34971
                     /locus_tag="PK224_12075"
     CDS             34768..34971
                     /locus_tag="PK224_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16520.1"
                     /translation="MQTDSIRIKIETDGTIKLITDEISLANHATAEDVIQQIITLSGG
                     SLQSSRRSTHNHHHHQADEHLHH"
     gene            34983..35894
                     /locus_tag="PK224_12080"
     CDS             34983..35894
                     /locus_tag="PK224_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16521.1"
                     /translation="MLNAQSAFSSLWSNAFCLMLTYRKLGIHRRMDSATVLSIDTEPS
                     RVRVTKSILSCPEYLAITRLYSRVRTKLEKLTLPAGLRSGMYLIPVSLASEVDTIIAN
                     AEDELRQLVDVFLAQYDKRIVEEESRLKAAYHTRDYPDPDTVRAAFGLTYSYITFDLP
                     GTLETISATMLKREERRSMEKVNNMLEDVNTYLREAMRSLVNHLVDRLSPSESGKSKT
                     FKSSTLTNLLEFLNTFDARNLGNDIELSQLVTTAKTVLSGVDSQLLRSDQSIANVVQN
                     GMGTIKAQLDSLLIKKPIRLYSFDEAA"
     gene            35953..37008
                     /locus_tag="PK224_12085"
     CDS             35953..37008
                     /locus_tag="PK224_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRB16522.1"
                     /translation="MSISPDAIAHARSINLIEFLISRGHKPVSRRPHHADFHAPYRED
                     RNPSFSVSQRPDGTWVWYDFGRNEHEGCKQHGDLIDLVQLLDHVPFAEALHRILHNTV
                     RSMVAPSNPSTAPPTDKERIAHAKKLYYAAQANMTPEREEELRSYFHKLALPYNQHLG
                     AVWMRLGDDGIPYVAFPLPTPNIHFMQGLMARALGEAPPALMRVARGLKGPWILKRGN
                     APILITESIVDCLAGDELFGPSFTLCALNGLNTVERLQEYLKRLPSRIIYVALDNDAS
                     CIAVGNALQNSSRKKGPHVQKELVSLLTHMGYHVMEVLLHHNASVKDLHKLWLKHPQR
                     VSLLDLAKTGLHHAPAC"
     gene            37232..38806
                     /locus_tag="PK224_12090"
     CDS             37232..38806
                     /locus_tag="PK224_12090"
                     /inference="COORDINATES: protein motif:HMM:NF025085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HRB16523.1"
                     /translation="MFSEDFYPTPGAVAAKMLQKIDRNAVHFLEPSAGKGDLAKAILG
                     FGRTRSPYDHGSRHRVDVIELQPDLLKILQANEELTVVGYDWLTYDGVSYYDAIVMNP
                     PFSKGALHLLRAWDFLHNGEIVCLLNQETIDNPYTEDRQRLAAIIAAHGSVEPLGPCF
                     LTAERPTDTQVALVYLKKTTEDDRIHLWHSADREQSVNDDIGTPEAIPALRDTLGNMQ
                     HYYTQSLEEMFKAFAHIRKASLFMGALGTELRPSRSTRDESDLKKILGLAQTNTTAAR
                     AEFARALRRSAWMHVFEQMDFRKWLDTKQTEELLQDLERDSTVPFTTQNIKGTLSNIF
                     QQRKRLFEKSVWNVFLTLTRHYKGNTTGDIGSGDGKAGWKSNDSYKVNCRLVFPYGCR
                     FWIGRFDLWSARDAGEIYSDLDRVLAVLDGQSFEEIVTVRDALERSFHNHGVHPHPCE
                     STFFRIRYFKKGTVHLKWKREDLLATFNTTAAAGRQWIGTTCHRDQDSQASYSHPDSN
                     DPHSWPSPVPTCQICA"
     gene            complement(39130..39849)
                     /locus_tag="PK224_12095"
     CDS             complement(39130..39849)
                     /locus_tag="PK224_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB16524.1"
                     /translation="MTTSVNSPINSPVKTISVVFADDHALVADGMTTILHGVNHKQRD
                     YEFAVVGQASSGRGLLELVKRVPADLAFVDISMPDMSGLDAVPKVREIRPDIKIIVVT
                     MHKEPEYMTRALSVGANGYLMKSCSAQEFCHAIDEVMRGNAYVTSLLGSRPTGALPGS
                     DDEEDQLASLGMSQRQREVVTLLASGKTIKETAFQLGLSRKTVEHHKAMFREKFGIET
                     DADLTKFAIKHGLTGTDWIQK"
     gene            complement(39846..41360)
                     /locus_tag="PK224_12100"
     CDS             complement(39846..41360)
                     /locus_tag="PK224_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB16525.1"
                     /translation="MKLNESLVVDQEKKYFEDVQELHQTDLKMRRIADAVPGAVYQYV
                     ITRDGCQRFSYISRGAMDMVGYPSDVIVSDYSAVWKLVLPEDMPGIMASIENAIRSGS
                     RWAHEFRLRLPDGRVKWLRGDSLPERPTVDGTVLFHGLLTDVTERRLAEAKLRFTQFA
                     TDHAADAILWAGPDRRIMYVNVRATELLGYSREELLNLSMPDIAPLQSRIDILNDLTR
                     LKQGEVVQYATTFRRKSGTVFPIEVSMRYLEHDGEGYVCAIVRDVSERKRAESVIQEG
                     AEALRRSQEALRALHSQLLTAQENERRRVARELHDDLGSRLGSVILRAEGKLVLLENR
                     SPEAAVIKNVTDELRGISDRVRTIAHELHPSILDNLGLSVALKKMLKEYGSWSGMKIV
                     SYISMEFTEALDREVATCLYRIAQEALMNVKKHAGAQQVMVSLAREEGWVQLSVKDDG
                     MGFSPETESRRTKGLGIVAMEERIHMVAGELAVQSGKGRGAVVVARAPYREREA"
     gene            42568..43383
                     /gene="traT"
                     /locus_tag="PK224_12105"
     CDS             42568..43383
                     /gene="traT"
                     /locus_tag="PK224_12105"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HRB16526.1"
                     /translation="MSRINRRTHCSVGRILASLSVVIATGSIVGCAGHMTLSHEVNLT
                     SSNAIFLTPSEEKTIYLQNRNTSDNSNINFAELPNKIKAKGYAIVDNPDKAQYILQSQ
                     VVLCNKLKPGQTVDALIAGGFGGAIGVAAGSAAALSGASLSQIPILGAVGAVGGFAAS
                     KFSEDSIVACVVDAMVQERTKEEIQQSVTTNSMPGPGTPQSQPGAFGFLNQQSVSPPQ
                     AGQVTQQISEIRKGTRRFHQARIVASDQKMWLSVPEASKILSDKLEDSLAGLF"
     gene            43473..43781
                     /locus_tag="PK224_12110"
     CDS             43473..43781
                     /locus_tag="PK224_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16527.1"
                     /translation="MLLFTIIMLFLLALITVTSKRYGFRETYLIAAGSAAGMLTSFMM
                     ALIVANIRQTNEWHSYPFEFFMDDPILAYWTSLFTWTTMWIVILYKRTISLEPSDSSS
                     "
     gene            complement(43848..45242)
                     /locus_tag="PK224_12115"
     CDS             complement(43848..45242)
                     /locus_tag="PK224_12115"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HRB16528.1"
                     /translation="MDVDLTPLSELDEETLSPVGISAELVFPGTLTDPGSLSVTTPDH
                     LLSEHILGSIPLWEAKGRPTGAKRASYQKQIYRDVWRNGRLCKVSLVVYGSGDLGLPN
                     SIDLEFFRGFERWTKAAMSRGEPFNQLVKISGRELLEASGKGLGGASYQEMDRFFLRM
                     AGTMIGAGRDWRQEGESGGTEKRPRSNKGIVFHIFQTVVLPGQVNADGFVADKYEVEL
                     ATWYWMSLRSGNCIVIDHELFRDLHGSITKLLHQLLHNLFYLGRGTASQKYSELVRNW
                     QIKRHSALSLVKQQLDEAHRELLEKEFISRWEYVPIRTPEGKEFEIIWEAGPAWWATD
                     KKTREFREEMVGSSQRELPQLGRELDPFLLIEQSDESSPEKDEEREASNARLLSIVLE
                     LSGRRKDPKVWEKWWKRAIASVPHPMIWRRIGEVKERRLSGQNLNVGSYLLSLIRRDA
                     ARLGLPWAISDKVK"
     gene            complement(45668..>46287)
                     /locus_tag="PK224_12120"
     CDS             complement(45668..>46287)
                     /locus_tag="PK224_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRB16529.1"
                     /translation="EVVAGERRWRACALLGKTSIPALVRRKDRDRAAAEALIDNVVRK
                     DLSALEEAKAYQALIERYGFQQSELAERLGCHKSRISKALSILKLPETILNVFFGRES
                     HMTAAHAEALLPLAHDEPRLHAIARRASKESWTRDRIRQEIDRKPRINEGAQAVRFVE
                     RGRGGDRGFMLTILFHSNKPHEIPMIEEALKQASERIQEFRDKLT"
BASE COUNT        11174 a        14456 c        10817 g         9840 t
ORIGIN      
        1 tttgatgtaa gtcgtcacat tctcgaccga aggatgatcc aacgcgcgtt ccttcttggc
       61 cgacaccagt tcacgcaata ccgtcatcgg aagggcgttc aacttcacgc cctcgaccga
      121 aagcccctta aacagagttc ggtacttcga cggttccagc caagcctctg gtgggaagct
      181 atccatcgta acgggcacag gactctcttc tgcgtcctcc tctgcctcgg ctgacgggtc
      241 ctgataagag aaccacccct gcctgggatg gtcgataaaa gtctgagacc caggaggctc
      301 agcggccagc acagcgctgc tcaccactcc caccgagcag aaggtcacgc aataccaagc
      361 tagagcatgc agcagttttg tcgtttccat agcagccaca caaaatcctc tccaacggct
      421 ggcacgattt tcccgagccc ccacatcatg agtgtccgtc ctagaggctg acagcacggg
      481 ggagggagtc ctctgtttgg aatcggccag agtaattgaa acttgtactg gcttttcaca
      541 atgagtgcgg tcgggatgtc ctcacacagc agcggcccat tagcggaggt ccaaaactct
      601 gttcccgcgc gggccagttt cgctaacaat cgcgtcatcg acagcgcggc aggtttgagg
      661 tccccatccg ctcctgagac atgcccagtc ggcggataca tgaatccaaa gctcccggcg
      721 caccagaaca gcggatcaat tgggaaaccg actgctgcag ctaccgaatc ggccgcgcag
      781 gccgcgagtg ccaccggatt ggccaggacc aatgcctccg gaaagagaat gagtgaaatc
      841 aggtcgtcgt cccacaaggg atcaatctcg gaggcatagg ccaaatcgat ttcgtctgcg
      901 atcccggtag gattcagaca cagaatgtcc attccgatac caacgatggc ccatagggga
      961 aaaacgaaat aatgcacatg aaaaaaggca taatttagct cggagtccat ctgtgtcgta
     1021 gccatgccac gcccgatgta actgaacccc ccggccaaag acacaccaag cgtcggaaag
     1081 cacagtggat cgtggaccgc ctcgaccaga ttcttcggct cccagatccc cagaggtagc
     1141 ccaggaatgc agatcggacc aagacacaca cacccacaca actgcggcgg aaacggggcg
     1201 tgtccaggac cgatggccgg ttcggtaaaa tcttctccca ggttaaacac cgtgaccccg
     1261 ctgagagaaa tgggaaacat gcactcccaa cacgtcgtag cgagactatt cgccaacaca
     1321 ttggtccctg tcgtacacac cgcctctgcg gagcccgacg gccacagagt cgccacgccg
     1381 gtcaggacac aactcaggca gaccagcagc agcatatgct gtattcgtct tttcgagagc
     1441 ttattcatga atcgccacct cctccacccg cagatgacga cctagctgag agacgatact
     1501 aggtaccgca ccgaccccca tgcgactgac aagcagaggg ttcgcccaat agacccgctg
     1561 atgaagttgc tccatcactt tctgcacgtc gccgtcagag atgatcagct tcacaaactt
     1621 cggatcatgc tctttcaaat actgctgcac ccaacctacc tgtgaggaac tggtgccatt
     1681 gaaaaacacc agagttcgaa tccaccgtac cttgtcgaag ggattaattt tggtgccgga
     1741 cttgattaag accgtgttcg tcgtggggtc cttaatatcc tcgggatact cgaccgttgg
     1801 atcaatgagc acgaccctcg cttcttcggc atgcggaagg gccaaccctg ggccctttgc
     1861 gaactgacgt ttgagggcgt cgccagaccg ccgttgaata tccggccaat ccgccttcgc
     1921 cacccgctcc ttcatcacat cgatcatgtc ccgctccgcg atgtcagctc cacgtgcgtc
     1981 gccctgcacc ttcacactct gcccaatggt cggaacctgt ggcggagctt cttccgtcgg
     2041 ccccccctga agaaaccctc ggtgccggcg ctcgaaccat tcgatccatg gcatcacacc
     2101 cgcctgttcc ttcgacaaca acgtcaggag atgcgcgacc gaagctcctc cgctcgctac
     2161 taggaatttc tcgtctttca caaacaccag ggccggaact tctcccgcac caacggtccg
     2221 gtaaatgact ggatcaatcc cgattccaac cagcagggag tcatctggat gatccccggt
     2281 gaagagcaga tgcttgatcc gctcctgtgt aaccttgaac gaatcctgca ccagtccacg
     2341 caagagcacc ttggcgccca gtaatttcgc ctctcgcagg tagtctttga gggtgtcgtt
     2401 cggtaacgag aaggagagta gaaggtagac gccgtctcgg cgcacttctt ttggtgactc
     2461 ccgaggacgc ccatgccgca acgcttggcg cgagtacctt gcgatgtcct gcgccatctc
     2521 ctcaggagat gggatactcg gacgtactgc taattgcgag tttcccgcga aaacagaaga
     2581 cccgatgctc agaagggaga gccctgcgac cgctaccaca cagacaatac cgctcgatac
     2641 gttcacggta cctccatttc agaatgtctg tatggagtat taccgaccta gctagatgag
     2701 tggcgtcacc gtgccgagaa tatccgcctt ccgaattagg ccatagtacc gagagtcgaa
     2761 cgaccgcggg tgtggcagcg ccacatagta ttcaccttcc ggaataactt ccggaagtga
     2821 cgctagatcg atccattgcc cgtaacgatc gatcccaaat ccacgcccca cggattgccc
     2881 gttgatatag acctcgtcct ctttgatcac gatatggtca cccggcactc caacgattcg
     2941 cttcatccac cgattcccca cgtccgcata gtcacggtgt aaatggcctg gctgcacgcg
     3001 ttccacgtag tcccgccgca tgatgaacgc cacgagatcc cctcgtccta cgggctgccc
     3061 tttttgaatg aggaaaaaat tatggtgcgg ccaactcccg ctcatgcgag cggcgacctc
     3121 ataccaaggg gcgataacgc cggcagtaat aatcgcacca cagaacaaca tcaaccccac
     3181 atgcgtccag cgatgccgat caagccagtc gaggattgtc ggcgcccgct tgagcacgtt
     3241 cagcagcgat aatgcattga atagcttcat cgatggagag ctcctttggt ccttcttttc
     3301 tcacgcgatc aatcatcgac agttcatccg gattcgtcgt aaacaacagc tgtgtctcac
     3361 gcggcacggt tagcctgcaa atgcctctcc caaatggggt gatcataaaa atctcagagt
     3421 actccgtggt ctttgtgacg gatgacagga gtcggtattc catttccgac aatacaatgc
     3481 gcccattgtt cctaaacgcc gcgatgcttt cggtcttttg cttgagcagt cccacaaatt
     3541 ccgcattttc aagcaaatag cgcccaacat cccgcatctt gtcatagtag tcattgatgc
     3601 cctgcgtgat ggtgatcatt gagccaccgg acttccggac ccggcgcgcg ccggtttcaa
     3661 aaaacgcagc ggtattgcct ccctgagaca acaaatccca cgcctcatct agaatgacga
     3721 atttccgccg gccacggttc tcctttttgt acatcactgc ctggatattc atctgcatct
     3781 gcaggagaat gactgaccgt aactgcttct gttcattcaa tccgtcgagt tcaagcacca
     3841 caaagtcatt ttcaaaattg acgttgttcg ccccgttgaa gagatgggaa tacatacccc
     3901 ccttcgcata ggaagccagc acacgcgcga gatctctggc tcgaggatca gtcatgctct
     3961 tcaacacctt gtgtaccgaa tccggacatc cctcagatcc ctctccttcg agtacgcgca
     4021 tgatcgcctc ttcgatgaac gagagctcga catcggagag agggcgcgaa ggtgacgcca
     4081 tttgcgcgtg caacgctttg agggtcgcca gttcatcggc tcggtcactt ggatcgtcaa
     4141 ccccgccggc attactcccc caatgcacaa gtttgctgaa cggattaaaa caaaggcgtt
     4201 gagcgtttgc gccgtactgg atgtattgcc ccttgagcaa ctccacaagt ttgacgtagc
     4261 tgaagccagc atcgatgacc cacacctggc caccaatacc caagtagctc ttgatccaat
     4321 tgttcataaa gaacgattta cccgcccccg atgtcgctgc gatcgccatg ttgtaattcg
     4381 tctgttgatt ggcgaacaca tccatggtga tcaattgacc tgaacgagac gtcagcagca
     4441 ccactggagc tccgcccccc ttccagtcag ccacaatggg gcaaatatgc gccgggacct
     4501 cggtatgata agtcttcatt cgtcgcagac catcaatgag cgctttgtcg tccgacggta
     4561 gtccgagggg tagacccgtc ataaggattt tccatccaat gaagtgatct tcctgcaagt
     4621 tgatatcctt cgcccgatag agagctcgaa gggcgcctgt ctgctcctcc acatgtcgcg
     4681 gtgtatcccc atacagaacg acctggaaat aggaccctat gagctgtctg ccattttcaa
     4741 gttgcgcttg gacgccattg aaatgatcca atttgaactt gagggaggga ataagcccca
     4801 gaagaaaccc actggcctgc tgattaataa tgacgtgctt ctttcgtaac ttgcctttcg
     4861 ctttgacctg gtcaaatttc aacacattca gcgtcagaaa aaatggacag gtaatttgat
     4921 cggtgaaccg gatcaagctc ccaatcaact cgcgattgtt cccgccatgc cactgcacag
     4981 gccaggactg aacagtcagc gtcttgagaa actttccgtc cagttcaatc atcttggtgc
     5041 gaagcgtcgc cttcgtatcg agacggaccg cctgatctcg tatcggcagt tggtcgctgt
     5101 acgccggcgg ccgactctcc cagctgtggc ccggattgag caacacatgc aagagactca
     5161 ataattgcag cggccctacg cgctcgggca ataatcccaa cgtgttcaat tgcccctcaa
     5221 tgcccgacac cgcttgatga atccgctcgt cttcccatcc ttgcgccgtc gtttcctgcg
     5281 ttgggaacgc cacggataca tacaccgtga aatctcgtgg gcgcagagtt gggttagacg
     5341 tcagttgtgt atctctggca tgcagataga actctgcggt ctttctcgca tcagctacat
     5401 aacgttcccc tgcctgacct cgttgagcca gaatcacgtg attgtccaga tatggctcga
     5461 taatggggga cgcatgaagc atgaattgaa acgtggttcc aattggcagg tccgactcga
     5521 agagattcgt cagcttctga atagactctt ccgacaatcc cagtaggggc ttgctagaaa
     5581 acaccacacc gatgcgccca tcatccaatc gatagacctg attggtgtca tcccaagcct
     5641 catagggcag ccagtcactg atggctgtcc gatcgaccag ctgctttacc ttccactcaa
     5701 ggatcgttga catattcgct cctctgggtt ccttccacta ctggggtggc agcggcccat
     5761 catcggtcag ataattggac gccccggtct gagggagctg aggaaatccc tgcggcatga
     5821 tgccacccga gccaagaggg ttcacggaca tcggcgccga acgttggcca acttcaggaa
     5881 cattctccgt catggcgttc gccgccgcat tcccactggg tgttctcttt ccgtctcgtt
     5941 tctcgaccat ccgccccatt ctcggataca gatcctttcc atgccccgaa ctcgacccag
     6001 cactcacccc ctgcgccatg tgtccgaaaa taaaatctgg ttcgtcgaca atgagatagt
     6061 gccgcgactg atcatggagg cgctttttcg tatccttcca tggaaatacc gtcacacgaa
     6121 gagccgacac cggcgtaatc accggcttgc ctatggcgct gtccggcttg ccgctgaaga
     6181 tcatcggagg agtgacctga ccagggccag agcttgaact ggcgtcttgt ccaccgcctt
     6241 tttggtcttt cgtgtactgc gtcacctgct gcccagcaga gaaccgcttc tcgtaggctt
     6301 ccgatacgga ctcacacgtc gcctgatccg gatacccctt acaactgaaa ttagactcat
     6361 agcccccaca gcccgccaga aggacagaca ggcccatagc taccccagat cccaagccgt
     6421 accatgatcc gcctcgtgtg atgtagcgcc tgatataccc agacatcatg tcgcatgcct
     6481 cctccagaat ccgcccactc gacttctcta cttctgctgg gcctctatct gcagtaaagc
     6541 caatacttca ctggcaggac gatagccggc aaacgattgt ccatttggca aaaacaccat
     6601 tggggtggag ttcactccca aacgcttccc caatttgatg ttctcgtcga tcggatgact
     6661 acaagcccct acctcaccca ttactggttt tgacatcagc gcgcgcccca gcgcctcggc
     6721 ctgatcggga gcacaccaaa tggcgacaga cttgcgatag gcatccggat gcgtccgtac
     6781 cagtggatac agaaccacgg ccaccggaac acctgcctca acaagcttct gcacctccgg
     6841 atgaaactgc tgacagaacc gacagtctgg atcgtcaaag accagcaccg gacgctccaa
     6901 cttcgattgc atgggggaca acagaatggt tttcgaggta tccattccgg ccaagagcgc
     6961 ctgctgacgg tctctcacat actcttgcgt aacatttcgg cccatttgaa tatcgaagag
     7021 tgccccggag agcacgtacc gcccggactc atgcacgtac atatagtttt ccacccggtc
     7081 actgtcgctc tccaccaaga gccatccatg aatttccgtc ttggacactc gcaacacttt
     7141 catgccagga aacttttgct cgatcaccgt tttgatagac ctcacggcat catccgtttc
     7201 ggctctcagg atcgactctc ccgcaaccga cacacacacg gcacacacca gccccgcgat
     7261 tatctgctga taggatcgtc tcatcgccat catgccccct ttcacactac gaattactgg
     7321 aagaacccct ctaactcacg tcctgtccca acgatgaaat ccacttcccg ttgcgcgtcg
     7381 atttcaatca ccgggaaaat ggaggcagcc atcatgctgt agtggcgagc caacaattca
     7441 gccgcccgac cggcaccacc ggccattccc gacatcgcca caggcccggc cggcggtaag
     7501 ttgaaggctt ggctcgggct ctgtgcgacg aaaaatccgg actggtaggg cataaaggct
     7561 cgggagatgc ctgacacaaa accagcgagc agcgtgcgac ccaacaatgc cccctctttc
     7621 aagacaaccc gcccacgcat tccctgtttg ccatcctccc cactcacaaa ccctttcaat
     7681 tcgatatcta ccgcttcccc attcttcttc acgcacgaca aggcattgcc acgaatcatc
     7741 acgcgttccg aggacaaatc tcccaagcct tctcccacaa ggaaacagtc cttcgcatca
     7801 atcctcgcct cattcgggag cttcatgatg tcacgcacca tcatcaacac cgggtaggga
     7861 ctgtttgcgg aggcgccccc accctgcgcc cccgtcgaac gggccggtgc atccatgcca
     7921 gaaagcaatt tgacgggaat aatcgtcccc gtattcagcc acaatttctt ctcgttcttt
     7981 ttggcgccag gcactatctc tggccgaaat acctgaaacc catcggtcaa cccgcccccg
     8041 gccccaggcg ccggtagcgt cccgcctgcc ggtttcgggg tgctttgcac cggaatgggg
     8101 cctttcggaa ccggcatggc cgagacaggc gtcgcacttc ctccacccac gggattggca
     8161 ctgagcggag gcggcagcgg cggcaacgcg ccactcttcg tcccggctga gaccacgcca
     8221 ccagaccgcg ccactgactc aatccgcttc tgcaggtcac tcaactgagt cttaatctcg
     8281 gtcttgtctc gtccgtcctg ctccaacaat ctccgcattt gatcccgctg ctgctccaac
     8341 gcttcgatcc tggccccggc gtctttcata taaatttctt tgtcggtttc ctcgcgattg
     8401 atcgacacgg tcttggctgg agacttccca cggacaatcc ccggttgatt ccctcggaat
     8461 tgctgaagca ccatgctcag cacgatgaac ccacccacca gaaccactcc cgcgagcagt
     8521 ttggatggag cgggtttctc tggacgaatc gggtcgccag ggttgagaat cgcgctctcc
     8581 gtcggtttaa tcgatgccat cctaggtctc cggtctgagg ttagcttttt gcgtctggat
     8641 ccactttcgg ctcgggcttg ggtttgggcg gttcaggcgg tgcgtcatcc agcaggtaca
     8701 ccaccacttc ttcccctgac tcgaccacct cgcgatttaa ggcgattgct ttttgaaccc
     8761 cggtcaggaa ttcttgttcc cgcagtatgt atttgtgtcc cgcggtattc accaatcgat
     8821 acacaatcac ccggtacttc ggccctcgat actcgattcc ctgcaccacc ttgagctcaa
     8881 gccacttcgg ataccgttct tcggcgatcg acgtctgccg ataccctttc ggtaacgctc
     8941 cccgcgcagc catctgcaaa gtctcgagta gcccatccac atagtcagcg gcttggcgag
     9001 caggatccgt gtcatagacc acagtcccca cgcgcctatc ttcaatacta atggtctgcg
     9061 cgcctaacga cacgctcacc ccgacctcaa agacgtaggt ttgctcgccg gccatcacga
     9121 ccagatcagc ggggcccgtc ttcacctgga caatcaagtt gcgcccttcc ggcttcgctt
     9181 tgagatccgc cctagagctc gtcgccatgg taatcggggc aggaaaggtc aggacgttca
     9241 aatctcttcc agacaatgtg agggacgtcg caccctcatc accaaccacc accgcagctc
     9301 ctaccgactt gccccaagac agaaacaaca gcaggccaag gacgcacatc gccatcgccc
     9361 ggtgcttcga gtctcgatac acgctggatg tcatacgtct ccccctaccc ctttgccgga
     9421 tctgattgac tatcctgccg tgaacgatct aaatgaaccg cagagagccc aaccacccac
     9481 ggcctgccct gatcccaccg catcgagata cggtactccc gctcttcttc ccgtatctcg
     9541 ctctttccca caaatcggcg ctcaatgccc tcgaacacgg ccacgtcccc gtccacacga
     9601 ctagccgtca caaaaaacac acgactgaga ttgttggtct tcacgtacac ggcctccgcc
     9661 ccgagagact ctgacataag cccatagtgg cttgacggta cccactgcag aagctgccga
     9721 tggtgaaact caacagaggc cggcgtgaca ttggcaatcc agggcaacat ggccatggca
     9781 atggactttt tgtaatactc gtcgccgccc attgcgttcg cccaatattc ctgcgtcgga
     9841 ttgatcggca ccacatgaac cgcgacgttc tgccgaaaaa atccgttcgc taccaacgcc
     9901 accgctaata ctgttgccag ggcggcgaac accagattca agacctgggc gttatgcaat
     9961 tttgatttcc acagctcgat tgtcatcgtc gttactccat catctctcgg gttgttccgt
    10021 gaggtgatcc cgtcagctga aacgtgggca caccagacca ataggcccaa tggaagagga
    10081 atccctcgga tttcccgttc ttcattttcg acaaaatcaa cgtgctcacg atccccacca
    10141 ctatcaggta ggtcaggaac ttcgtcacaa tgccgagaaa gatgcacaca aagagcacgg
    10201 ccaactcatc aatctcccac caaaagatct ggggcagcct atcgagatat cgtggaataa
    10261 atgtgccgtt catctgtccc tcttctgctt cattgtgacg gcggggccat gatctgtccc
    10321 gccgtcacga caccatgcct gtaccgcccg cttacaacag tcccgtggta atggtcggaa
    10381 tgatgttggg gatatagaag gctgctgccg ccacaccgaa tccggtgaac atgccggccc
    10441 accggcccgc catcgccgat cccaacccga tcgcccccgc caccagggct aacaacgtcc
    10501 ccagcgaccc ctgcatatag ttggtcaaca acgtgaccag cggaccaaac gtcgcatctg
    10561 cacctgcgaa cgcgggcacg gccagaacca caaacgaggc aagcaccatg agccccaaca
    10621 agagactcat tcgccacatt tctccggtca ctctcttcaa gacgttgccc atactccagc
    10681 ccctcctctg gctcgtcgct cactgtttac aagacgtgtg gttgccaaac cgaatgagcg
    10741 cccttagttc attccagcat agccatgctc ctcctcagcc tctccttatg gttttactga
    10801 gtcgcctgtg acaccagtgg acacaacaga tgttactgga ggtatttggg atcgttcctc
    10861 aaccgatgag ggatctccac acctacctcg cagcagcatc gtgatctcca cgcgtcgact
    10921 gtctgcatga ttcggcataa agcagcagcc tccgcggccg accacttcca cctgagtcga
    10981 gaccttcaag ccagcctgca atgctttcgc caccgcgtgc gcccgagccg tcccaagttc
    11041 ttcgtttgtc tcccgagacc ccgtcggatc ggtgtatccc tccacccgca gatagaagac
    11101 ctcctctgtg gacagttccg ttaagtctcg ttgcgcacgc tccggtacag cagccgaacc
    11161 catggcaaac aaaacggttc gatgccgcac ctcaggatcg cgacgacagg cggacgggga
    11221 cgtccttcct gtgaaaccag gaggtgctgg tttgtccacg ctgctcaccg cagcgcgaga
    11281 catgtcacct tgacggcctg ccagcgcacc cgcagaaaac gtcggcaatg tctggagccg
    11341 atgcatcacc gccttaatcg gtttcggtgt gagatccgcc accaacggtt tgtttgggac
    11401 ctgcgtcagg ggtgttggct ctgggcatcc agcgggacga gacagaagcc attgcggacc
    11461 agattggacg gaaaccaccg tactcgggaa ggtgtagcct tgcccgcaga gtcgcgttgc
    11521 ctcggacatt gttggcgacg cagcaggtcg cgtcgtacag cccccgacgg tcagcagtcc
    11581 catacaaacg agaccggccc atggcatgta agtcgtccgt ccccctgtca tactcccccc
    11641 tttcaccacg gcttccgagt cgccaggttc atctttggta ttaccgactg ccacggtgta
    11701 tcgaacgaaa acaggggttg ccggatatcg gaagggacgt cgtcgacgcg acgacggaac
    11761 caagaaaaga cgccagcggg ttaagaagcg atcagattaa cagtagccac ccacccaccc
    11821 caggcccgga aggtcccgtc gggacattgc tggaataaac gcgcgcactg aagacactga
    11881 tgaaggtacc tatccatgaa ttcgtcacca tcgtgtgaac gatacccaaa caactctccg
    11941 ttacaacgag gacacggttt caacaaattt gcatacagtc ccattgtttg ctctcctcgc
    12001 ttcactacaa gacactcgta ttattatctg ggataactgc atgcatcacc ggtgcatgga
    12061 tcagcgtgcc gagggtcgcc attctggagg caatcctcgc gattgcctcc tgcgactcac
    12121 gcggcgcctc caattcgacg cctcgctgct gagcttttcc ttccagtgcc tcgcacaaca
    12181 catcaagccg ccgcacaatg gttctgacgg ccatctcttg aaattctgcg gattgcgtac
    12241 aggcccccac gaacccttcg gcttcgagat ccgtcgatct ttccttgcca atgtcctgct
    12301 gccgcgtacg atgagcctgc acacgcgcct cgatacgcaa gaccgcactc tcaaagcccg
    12361 taattcagag tcgttggaat acgtcacctg ttcggcattc caggcactat ccatagtcaa
    12421 ggccggcaga cagaatcgga cgagataact ataggaaagg ggaaagctca attgggcgcg
    12481 cagggcttca atccgctccc actaccctcc gaaggcgttg tagatcctcg cggactcgta
    12541 cagcatgtgt ggacgaatac acttcccgac cgctgcggac atacattctg ccatcttcac
    12601 gacgacagca tccccatgct ggctccgttc cagggcttca accactatac cgccaagccg
    12661 aaagcgttca cggatcaact ctcccgccac ataggtgatg gacctcgcac actgttcagc
    12721 ccgttgcacc aacgattcct gccaatcagc cgcgtactcc agcatcagcc ctcctactgc
    12781 gctagaaggc atagatcact cccactgacc cacgaaacgc cgcttgccca acagatccga
    12841 ggaaatcacg ggaggcactc aacgagaaca accagtgttt gtccaacgcc aagacatacc
    12901 ccagctccgg actagcgact tgcttcttgg aattgagcgg gtcttcttgg aatcgcaagc
    12961 cacccacaat cgcagaagat tcatccaggg cttttacaaa tcccaagttc acaagaataa
    13021 tgctcgaacg atgcgggacc caatggcctg gctgaaatga cggcacgacc tcatatccca
    13081 gattgccatg gagccaccac cgattcccaa gattccagga cgtgaccaac gccccgcgca
    13141 agccaggatt cgttcggtct cccgcatagg gcacctcacc caatacgcgc acgctaatcg
    13201 atggcagcag ctctcgttct tcggcctgtt tccggaaccg cgctaaaatt cccaactgga
    13261 tcgacctagg atcgtccatc gtcccaggat tgagcggacc tcggagcgac gtcccactga
    13321 ccgtcaattc catattttct gtggcgccat agcgaatatc gtgagttgcc cggaagcgcc
    13381 cactctcaga gcccgaatta ttccgcgtga tgtcgggccg gatcgccgac tggatcacca
    13441 cttgatctgt ggacgccggg taggcgtcat cgagagacgt agggtagccc ggactgatat
    13501 ttgccgggga gagtgccatc accacgttgc cccccaggca tacgagcccc agggcccccg
    13561 caaccgccag taaccacttc ctgcctccac gcattagtcc caagctcctt ataggaattg
    13621 cacaacatag aaggttgtta ccctcgtatt accgaaggac caaggacagg aaaggcagga
    13681 gattttggat tgagtagacg tcaggggcag aggaaagaag atgtcagtgt cacgccccct
    13741 gacagatccc taatggccca ttccagggac catgagccag gcacaattcg caaggagagc
    13801 cgtggatcaa cgcgatccat tggccgtgtg atccggtaat tcctgtatga gtctgcacct
    13861 cagcccagca ggcagctcaa taccccgttg aagcttttca cagagcgtgc ggacatgatc
    13921 gtgctcactt ctcagaggca tcactagcag ttagtcatga agcgcataca agcctacaaa
    13981 tttgaattga tgccagatgg ccagcagcag aaccacatgc gtcgcttcgc tggttcgtgt
    14041 cgattcgtat ttaatagggc tttggcgctg cagaaagagc gctatgagca acgcgaaaag
    14101 aatttgggct acgccggctt gtgcaagcta ctcaccgaat ggcgcaacag ccaggaaacg
    14161 gcatggctgg ccgatgcacc ggtgcacccc ttgcagcaat cgctcaagaa tttggagcga
    14221 gcctatgcca attttatttc ccagcgtgca gcctgtccac gtttcaagaa gaagggccag
    14281 agtgagaggt tccgctatcc ggatactaaa cagatcaagc tggagcaagg taatagccgc
    14341 ctatttctcc caaaacttgg ctggctacga tatcgcaaca gccgcgaagt actcggcacg
    14401 gtcaagaacg tcaccgtgag ccagttgtgc ggaaaatggt ttgtctccat ccagacagag
    14461 cgagaggttg agccgcccct ccccaatggc aacgcagtcg gtatcgacat ggggatcgca
    14521 cggttcgcaa cattctctaa tggcagctat ttggccccac tgaacagctt cacacagcac
    14581 gaaactcgcc tgcgcaaagc gcagcgagcg atgagtcgca aaaccagatt cagcaacaac
    14641 tggaagaagg cgaaagcccg cattcagcgc atccatgccc gcatcggcaa tgtccgccgt
    14701 gactacttgc acaaagccac gaccgcgatc agccacaacc acgctctcgt aatcgtcgag
    14761 gatttgcagg taataaacat gtccgcgtca gcgggagcca gcctcgaagc atcgggtaga
    14821 cgcgttcggg ctaagtcagg cctgaacaaa cgcattctcg accagggctg gttcgaattc
    14881 cgccgccagt tgcagtgtaa gctggcgtgg aatggtggat ggctgctcgc tgtgccacca
    14941 cggaaaacca gccgaacctg cccagactgc ggccacgtct caaggcacaa ccgacagact
    15001 caggcggaat tcaggtgcgt cgaatgcggg ttcgagggcc acgccgatgt ggtcggggcg
    15061 attaatatat taagggcggg acacgcccag ttagcctgtg gagaggatgt cagtcccgcc
    15121 acacctgcaa aggtgaaacg tgcagcctct gcgaagcagg aacccaccga agcgactctg
    15181 gcgcggtttg atcactcgcc tgagcgccgt aagaatctcc ggtcttcagg ccggggagga
    15241 tgtcaaaatc ccaaatcgtt cgcacatatg ccagaccgca tcctgcatcc ccgggagatc
    15301 ccgtgtgata agatcgcccg gcacatactc cacaatcgca taacactccc cctgaatcgg
    15361 taggtacggc tgcatcccct ttagctgagt cctaaactcc cgttccacat tcgtcagcca
    15421 ccgcatgaaa cgcttcgttg gataccggcg aatccggcca tcgacaacca acagacccat
    15481 ctgactcttc cccgacggac tcgtatcgtg atattccaat gtcagagtac ggtccaggct
    15541 ccgtgtcgcc atcggcttta ccactgtggc tccgaaagca cgcaaggtca tgggcctccg
    15601 ctgctgtaaa cctcgccatt gaccgggcct catgcaccag ccctcacgct aatacaccat
    15661 acagactgtc gcctcacgtc cacgtgttct gccaacatat tgcatgcgct cctcgactgc
    15721 aaaggcccca cattcccgct ctgaaatgat aaacaccacg cactggtagc gactcccctg
    15781 cgctgtatgc acggtaatgc aatagcttaa ctgcaactca tccgccctga gtgctacctc
    15841 gcgcccagag tccaggcgca acgctatcgt gtcacccatt cccccaagca cccgcccttg
    15901 ctcaccattg actgctcgct gaccgccatc gtatcgagtc ttcgtgacaa tgacgcgatc
    15961 gcccgttcga aacccaccgt gacatggcag tccgtccgga ttcacaatct cctgcagcaa
    16021 tcggttcaac cgatccacac caaggcctcc tgcgtacaca ggtgacaacg cctgccactc
    16081 aaccccagtg tcttgatagc cacacaccac tttccgcaac tgatgcagca aaagcgcctc
    16141 ggatgcgaag cgaatcacct ggaccttgac cagcaacgac cccttggatc cttcttcgcc
    16201 caagtgaacc acaggcaaga cagtcaacag ctccgccatc acaggagtcg ctcccaccgg
    16261 ggctagttgc gccggatcgc cgatcagtac gatttgcccg cgacagctca acaacaatcg
    16321 atacaggata tgccaggtca gcatgccagc ctcatcgacc aagaccaagt cggcacccac
    16381 actcgacacc gaatagccgc cgcctccata ccccagcaac ttgtgcaacg tcgtggcgtc
    16441 ttccccgagg agggtcgccg ctttcccggt catcgccgtg gccgccaccc tccctcctcg
    16501 ctcacgcacc tgagcactga gagacctcgc tagctcagtc tttccacttc cagccccacc
    16561 ggtaaccacc gtataccgat agcgcaacgc cctggcttgc acgctgggac tcgcgttacc
    16621 tgagtcctgc tgattcgctc gaatcatggc tagggctttc tgccgcacaa aatacacccc
    16681 tggggaaaac cacaagccct gctcgcattt ccccgcacgt cctgcctgca gtgtgattga
    16741 cggcgctacc cgatccgcag gcagtccaat cagctctgcg caccgtgcca caagatgagc
    16801 gtcagaaatc cctccgatcc cttcttcatc ggcttgtgca agggcctcca tcactgcggc
    16861 cttatagtgt tcatcactat acaagtcgtg ccccaacacc tgtgcggccg ccaccgcagc
    16921 ctgaaatggg aggtctcgac gtcgatatag tagaaacgga tctgcagcga tggcctgcac
    16981 cgatcgtagc gttcccttga cgagtttgcg cgccacggct ttccatcctg cacaacacag
    17041 ccattccaca agcgggccca acaattgaaa caccttcacg acctgacgtg ggggcgaacg
    17101 acgcaagccg agcaccccca acaactccga tgagctcgcg caggcttcga atcgtcgtga
    17161 tcgtatcctg gacaccatca gaaatggatc gggcccattg gctatcactc gcccccagca
    17221 ctctcccacc tccccgaccg aagggactga cagtccgtcc ggaataaacg cccgatgctt
    17281 gcgagctatt tcgatccaat caccagaccc acggcgcgca cgggtccgac tcgtgatcac
    17341 cccaaccacc ttaggcattt cctcaggact gcccgccatc tcggctgaga cgacctcccc
    17401 ttgcacgtgc acttctaagg atccagagca gaccatgctt ctccctcccc atgcttcagc
    17461 catcacacac tcatctctgc aactctgtga cataccgttt tggaaccaaa acggtatgtc
    17521 acagctcgtt cacttcagcg aaccgcacga ccttccggtc gatcgacgcg ctgcacgaac
    17581 tcacccggag gccctatgct cgctgaactc attcatccac tcgctctcat ggaacatcag
    17641 gaatgggagt accggcctcg tccctcgaat gccggtccag accactgcat ccgcaaactc
    17701 acgtaccatg cccggcatgc tcccgcaaaa agtccaagcg gtcgattcct cgttgtcctc
    17761 gacgacagta cttggcacga agaactggtg ttaaactgga ttgctaagag cacgtttcat
    17821 ctccacagcc agcagcttga actgacctgc gcgaccacga tcttccaggg tcagcccttc
    17881 ccaattaaag gccacattga cggcgtcatc acggacctct tgggaattga tcgccttcta
    17941 gagttaaagg ctgtcgaaca cttcaccttc actcgctacg cagaagggaa ttacccgact
    18001 aactatctcg tacaagtggt cttctatctg gttggtttgg ccgcactgaa tcctcatatc
    18061 gacgaagctc tcctgctcat caaaaacaag aaccaaagcg cattccttga atatcgagtc
    18121 cgctacgacc gggacaacga ccgcctgacg gtcctggaaa tcacccacag tacaggcaca
    18181 cgcgtatacc cccaccaaga cttcgtgggc ttgtatcaag aggcactccg acgctttgaa
    18241 gaggtggaag agtaccgtga tcaggacgcg ttgcccccac gcccgtaccc caatgccgac
    18301 aacttccatt gcgcctactg tccgtatcag ttttgctggg aaggggtcga ccaggtcccc
    18361 attgagggaa tcgcggaact tcgtactggg ctggtgccgg ccgtggagga gtatctcgac
    18421 ctacaaactg agcttgcagc gctcactccg aaacaaaagc gactggacgc cctaaagcag
    18481 caatttaagt tggaattgcg caccgagggg atcacgcgcg cggaggggca tggctactac
    18541 atcgcgtcat caccctatga acagagcaaa ttggatcaaa ccaaactctc taaagccact
    18601 attgaacgcg cgaccgtgaa ggaaacttac agccgtttct cagcaggccg actgtccaaa
    18661 gtcgtcgacg caaccaaacc gaaacgccgg tcccctctca aaaagcctaa accgacgact
    18721 caaatcgcgt aactgtattc atcagctgat tatcatgact aggaggcatc atgtccgacg
    18781 cagacaagcc cacgttcatt cccatgaacc gcattaacgg catttctaac cgacgccgca
    18841 tgcctatcat cggcaagatc cgtctcggcg taaaagtgag cggccccagc gggttctacc
    18901 ccaaggaaac gcctcatttc gtggtcccag aagaagtagc caaggtcttc ggggcgcaac
    18961 ccaaggagtt ggaggtgatg ttcgtctcca acgatcccac ggtcaactgc ccgcaagctc
    19021 tccgctggta tggagaatcc gccggactac aatgcatcgg gaacaatcag gtggctcgtc
    19081 gcctgaatca agacacctgg aaaactgaat cgcgatcttg tccgtgcgag aaactcgagt
    19141 ccggagaatg cggaccacgc gcaaatctca tcttcatgat cccgaaagtc agcagcggcg
    19201 gcctgtttca gatcgattcc agcagtatca actcgatgga gaagattaac tcctatttcg
    19261 atttcctcgc cctcaccctc ggacgtattt ccatgatccc gctgaaactc cgtcgcgtgc
    19321 ccgtcactag acgccgcgaa ggacgcccga ccactcatta tccactcgaa ttgcgctatg
    19381 aaggctcagc ggacgagacg accaagcacc aacaggaaac ggagacggtc ctgagaaaga
    19441 ttaagatgtt ggaaattcag gagccgattg aaatcaaccc ggttctggat ccaggcgcac
    19501 acgtcgtcat ggaagaggag ttagaagaag aacccagccc cgtaccagca gagtccgtgg
    19561 gagagcccgg gtctgagacg cacaatccgg cgatcccacc gatgtccatg ccggagacag
    19621 ccgccgcgaa agcgccggag gccactcccc taccagcagg tccgcccatc tcactggccc
    19681 agaccaacta cctgcttcgg ctaaccaagg acttgccact cacagaggat gaggtccgcg
    19741 tgaaagtccg cgcactcaca cagcgcgaag cgtcgggact catcaagaag ctactcggtg
    19801 aagacttttc ttttttcaca cagagctcga tcgctcctcc ggccacgcct gtgaacacag
    19861 ggtcagcagc cgataccatg accgatcctc agtttaagaa aatcatgggc ctcgtcctct
    19921 cgatggatat cccagaaccg cacgtgaaaa gtctcaccgc ggggtacacc aaacgacaag
    19981 cctcgacgct cattgaccag ctcgttggtg gagatttttc cgcattccag gagatcaagg
    20041 aggaagaatt gccagtcctc acacctccga aaccaactcc agtggaagaa cgggtaggat
    20101 tctaaactct ttggagatat ctcctcatgc cactatccga acagtgaaac accgcaccag
    20161 caaagttgcg tggcggtttt accgattcag tattggttca gtcccatgca ggtgacctgc
    20221 cccgatgtgt tgaacgacgt ttcgcgggct tattggactg ccttcttgcc gagctctcgt
    20281 gctcccactt ttgcggcttc ccaagctaat tccttgcatt ggtccggcca ataccaacag
    20341 gcaaaggcgc caactgcaac agcagcgagt cctaacgttt catccggagt aatgtttgtg
    20401 tcggattggt ttcttgcaga agattgctct gccaaagatt cttttctctg tgttggggga
    20461 gacgattgtt tgacggccac ctcgtttttt ggatcgaaag catattttgc taaatctggg
    20521 aaggatactt tgttttccaa tgcgatagag tagatcctgc gaaaggactc tacagtttcc
    20581 tgtggaatgg tacacccccg cacttggcgc acccagtctg cttgtgaatc ggtttgagga
    20641 tattttctgg tccactccca aatataaaca tcaaacttgt ctttggttgt accgcactca
    20701 ataggaactg tgaacttcac tccatccttc ggaggaaagt ccgtaagagc ctgattgtat
    20761 gcaaccattt gcatcacatt cttagctccc ttttgaacaa gaccgtcata caacctgaaa
    20821 gaagtccatt ggtatggcag ccacgcctct tcactatccg agggggcgga gaatgcgagc
    20881 tgatatgcta aaagagcata ctttgcagct tgttgaatgt cttcagccgt agcagctctg
    20941 tccttcagca agtgcgctaa acgagaagct gataaaaatc gattttggct ggcagccatc
    21001 gtgaggtgat tgacaacttc gctagaatcc aaagggacaa cttttttgtc taagattgct
    21061 aacccaactt tttcagcagc ttccggatcc cctgcattgg ctagggaccg ccaatacatg
    21121 gcagctcgct ctagatttgg agctgctagc ccttcgccct tttccaaaat gtcagcagca
    21181 tttcgcttgg caatatcaac gccgccctgt gcagcaaatt cataccaaac gagggctgcc
    21241 ttattgtctg gcttagcacc tgaatatcca cgagataaaa gtgtgccgac attattagca
    21301 gctaagagtg acccgagatc agacgccttt ttataggtct caattgccaa ggactctcgc
    21361 ttccctgcgt cgagcgcccg gccgtactga tatagaattc ttgcattgtt aggttgagtc
    21421 ttaactgcag cctcgcacgc agggatagct ttctcaaaat cgatggtttc gtacctgaca
    21481 ccagtaatct ctataggttt ggtggagtca aacactgaag ccgctagacg atcacattcg
    21541 cttgtggctt gagcggtttg cgccacagaa acctgaggag gaagcaggat aagtcctccc
    21601 atgatcgccc acactatcct gccgtacgaa ttcttgcgca tgccgacacc tttcgttgtg
    21661 aatacggatc aggcctgatg ataacgcctg ccaagaattg attgagctat cgtggcggct
    21721 tcgtatacct gattatttaa tttttttcta gcggagatac cgagaggaga gtggcgcaac
    21781 gctgaaggtc aaaaattatc tccgggagaa caccccaaga acacttcacc cgaagaccga
    21841 gagccggtga acctgtaact cgttgaaatt tagcctcgcc ccacaattca atttgataaa
    21901 acagcggggc cataaatggt tggctttaga caacacgcgg gggagactgg agtgaccaac
    21961 ggacggagca ctgccgtcct agagagtccg cagcgcggaa taggttactc atggggcgcg
    22021 gcttgcggca taccatctga aaatgaaagg cttccggacg cctccccaaa acgcaacaat
    22081 tcttcctgcg tttctggacg caaagaccca aatatttgga ggatattcac acagtttgga
    22141 ttgcgtccga acaataatct cgcggattca gataaatacc cggcatccac caggtaggtc
    22201 tccgaagatt gccctaagac ctgggctaag cgtgccactt ccgcttggct caaccgacca
    22261 atacctgaca gcatctgctt cagtcgtcca cccagctgca gctgctccgt aacacgagaa
    22321 agatcctcgt ccgaatattg acttacgtct tgccggaccc gctccaaaaa ggcagcaggc
    22381 atccccgaaa gaatctgatc atccaaaagt tccgcaactt cgggcgaaca cacctcccga
    22441 gctcgggcga gcagaagttg atacgttcgc cgttgcacat cgagcgggga accacctagg
    22501 cactgcgcaa tccgccgcaa catatcatca ctcgggagaa tctttttccg cccgctgctc
    22561 tttccccctt caataatcga tatgtgcgag gcagtacacc ccaatttttc agccaactcc
    22621 gtttgagtta gattctgggc aatccgcatg cggactaaca aatctttgac cagcaccata
    22681 cccgctgcac tctcccgata agacttagac agatgcgcaa ccgtcgcttg cgaagtgatt
    22741 aaacgcttgt tcgcaaatta taggaaaacc ccaatctatt gtccatacgc ctatggggat
    22801 tactctacct tcccaatttc agaccccaaa aaacctgcgt atagtcctgc tgacattccc
    22861 gagggcagta acacagtgat gaacaaacaa acatttgttt atgtttgtca tcaccactta
    22921 caccaaagga gaaaggacag ccgcatgaat gtcacgtccc ctcctgttcc tgaagaagcc
    22981 ggaaagcttc tcgcactcat ccagtgcgaa ctcaacctca gtacgaaggc aatgtccaat
    23041 caattaggca ttaccgacgt ctggatgcgc cggctgctca acaacgacat cattccctca
    23101 cagcacctcc tgaaagccat cgtcaccctc tatgtcaacc atcccgatcc ctgcacactg
    23161 aaccatcgcc gggacctggc gcacagattc acacaagcct gcgcacgcac atattacgaa
    23221 cggaaccatc atcgtctcca gcaacgttgc cacatgctct ttcattttcc ggaactcacg
    23281 gcttcactca tctaacccca tcggaaagga caaccatatg tcgtttcgaa cacaagctca
    23341 acaactcgcc atcgactcag atgatacttc gccactactc aagctgtttc agcagagcaa
    23401 acccatcatc cactcgatcg cgaagcgcta ctgcaattta gatcgctcct atgaaattga
    23461 agatttagag cagataggat ttctggccgt cagaaaggcc actgagaaat gggatttttc
    23521 ccgtggcaac gcccaattcc aaaccttcct ctatcgctat atccagaaag cataccaaag
    23581 cgaatttgat ggccgggaca aattggtcga aatcacagat aaagacgacc aatacgtcaa
    23641 gacgctgccc tattccacat tcatcaaaga aaagcggcgc ttacacagcc agggctttaa
    23701 aagtcgcatc ttccgaaggc ttgttcctct ggcaactcac gaaccgcccg tctcccctga
    23761 cgcctcgcct gaatccctgg gactgcccga tctacccaac accgatgagg tgctggcatt
    23821 cacggccatc gatcggttca tcgagttctc tcgcaaacgc gccaggacga ttccgcagcc
    23881 aaccgccctc gatcacaacc tgttgaccga gtcggagcag accatcacgc gattctctca
    23941 tgacagtggc agctccgttc gtcatgcacg gaaacgtcga aactatgcca actgatacga
    24001 cacgaagcct cgcggaaacc atctgccggg aggtggggct ccacgcaggg cctactccga
    24061 tgcccatgga gccagttctg cccatgagcg ggttcaggga gatttgccgt gcccacaata
    24121 ttccctgcag tgattggaag ctctatcggt ggttaacctt ggaacgcgat agtctgccaa
    24181 ggctcgtgca aaccattctt cagcatgcac cggcccagtg gtacatcgtt accaccccgt
    24241 ctcagggccg ctatcgaggt cggcaatatg tggtccctct gcactatttg tccgcgtttc
    24301 atcgcaactt tgcaatccaa cgtagactga caaaaaacct cgctgagccg gactccagcg
    24361 ctcaacaaat tgccgatgga atcctccgcc gacagcccac gatcaccagt catcagctcc
    24421 aacgggaact tcatcgtcgc gggcatgagc tgtcccgacg tcacatttac agaatcatcg
    24481 ctcgtcacag tcagcaacaa ggagtccggc atgcagatca tcatgattcc catctcgcaa
    24541 atccgagtcc atgaagaata cgccaccctc tttcccccgc tcaccccaac cgagcgagag
    24601 agcctgaaag ttagcatcca agaggctgga attcacgatc cgctgcgagt cgaacgacaa
    24661 cccactgaca tagcctccgc gcaaccctat gtagtactag caggacacca ccgcctgagc
    24721 atcgcgcagg aactcaacat ccccgaagtg ccctgcatcg ttgtgacaac cttggaactg
    24781 aaggtcgccg cgctgttcga taacatccat cgacgccaat tgtcggacac tctccgccaa
    24841 caaatgcagg atgaggaaaa gcgctaccgc accaagctaa gggaacggct gattccagag
    24901 ctcttggatg tgcttcccat gctgcctcct gaagtgcaac tccaactcac acaatccaat
    24961 cacgatactc aacgtgattt cctctcaaag tggatggccc aaatacaaca ggtgagcatt
    25021 cagcgcttcc cggaaccaca gaacaacaaa ttcgccgcag aagaaccgga cacttcggaa
    25081 acaccaaccc ccaaggggca caaacagctg atccgccaga taaatcagct gaccaaggat
    25141 tttgaaagac aggtcgcaca gcagaagaag gaattagagg aagtacgaca acacaacgtt
    25201 tcgctttctc gtcaactgcg ggcacagact gacgacaagg aaaagttaca cgaggagctc
    25261 cagtcccttc aacggcaact gacgagcgac gatctcgacc acaaggccga gcgcattctc
    25321 aaactacagc gcaaaggtcg ctcccagagc ctgacgatcg acgaggcagg ccccctcatt
    25381 tctgggctgc tgcacacacg taaaaccatc caattgcttc aggcctttgc ccagcgcatt
    25441 gccaccttga ccacccctga tcagactacg attctcgaac atatcaagtt cactagccag
    25501 gccctaggag agctcgaaca agtgctcccg agccccacag cctcgccgct ccacacaccg
    25561 cgacaaacgc tgtcagtaca caaggggctc tctgctgata cctagtgcta cgtaagctta
    25621 tggggaaatc cccaagtcag cgccccaatg caacaggcat ttggccactt gtccgagaca
    25681 cctaatactt gtactactga actgtggaaa ctggagaatc tacacatgca gtgttcctct
    25741 cctgaatcgt tcgcatccgt cgatcacgcc caaccgggcg aagatcatcg gtctgtgctc
    25801 cgcacaacac tttccaaaat cactctgtat aattttgccg ccgcccacag tttgaataca
    25861 cccatcgaac tgaccgaacg actcctgttg tgcatcttat tggccaatga agaccgacac
    25921 ctctcgcgcg ccgagctaca ccagattacc aagatcaacc catccacgat cgatagcgct
    25981 ctcctcaacc ttgagcaacg caatgaagtg catacgctac cgcagcacgc ccctgaacag
    26041 accagctacg aactcacggc gtccggacac actgtagcta tctcactgga atcgtggacc
    26101 accagcttct accggtatgc ggaatgtgga tcagatgccg gggtacgaga tctgctccac
    26161 caactccaac gggccatcta tagccttatt gcgaccaaca caatcccaac aactcgggca
    26221 tgcattgctt gcgacttctt cgcgcctgag tcccaaacag gcggatactg tcggttcctg
    26281 agcacgccac tcacaatcca gacgaccagg cctaccgctt ctgacacccg cactccctaa
    26341 cccgaccaac gtccctcgtc tatcaattcc cgaaaaccgc actccgtcca gttctggtaa
    26401 cacataggta cttcatgtct atccatgacg ccaacacgac atccgtggtc ccgctccctc
    26461 tcgatccgtc gttgccgatg gaggatccgt ctgaaccatc ctcccacaca ctcccgtctc
    26521 aaagactccc agactggctg cacgcgcgca ttcagacacc ggataacatc acccaagagc
    26581 gacaaataac tgtctcgctt gatctcgcca cgatctcgcc cctcattcag aatgcgcaac
    26641 tcaatccaga gcaactcgcc gtggtgacag ccccagccga accgacactg gttttggctg
    26701 gcgctggcag cggtaaaacc cacacgctca ttcatcgcgt tgcacacctc gtcacccaag
    26761 ggcttccccc cgatcaaatc cttctcatta cgtttacacg caaagccgca gaagaaatga
    26821 tccacaggac gcaactcctt ctccagcaac actcattgaa gatccaaggc ggcaccttcc
    26881 acgccatggg gttgatatgg ctaaagcact atggaggcgc actgggtctc cctcccaagt
    26941 tcagcatcat ggatgaagac gacgcgcagg gactggtgca tttgttgatg agcaaactgt
    27001 ctttttcccg tcggaaaggg tttcccaaga aacaacacat tctggccctc tttagtcggg
    27061 ccgtgaatac gcttcgctcg attgcggata ccgtctccag cgatttccct tggcttgaaa
    27121 cacacacccc tcagctcgtc gttctgtttc gtgcctatac ggaaacgaaa tggcgccaac
    27181 atcgcgtgga ctacgacgac ttgcttttcc tggtgtacaa gttactctgc ctccatgagc
    27241 ctacccgtcg cagactcggg acacactatc aggccatcct tgtcgacgag taccaggaca
    27301 cgaccaaact ccaatccgag attattaggc tcctgggcag ccaccatcac aacgtgatgg
    27361 tcgtcggtga cgacgcccag agtatctatg gctttcgcgg tgcccacacg aaaaacatgt
    27421 tggagtttca agacgccttc cccgacgtgc ggctctacaa actcgaacac aactatcgca
    27481 gcacagctcc tatcctcacc ctggccaaca ccatcctcca tgatgcaaag gctctctatc
    27541 cgaaacgact cttcaccacg aaacagggcg gccaaccacc ggaactaatc gcctgttatt
    27601 cagaacaggc gcaaagcgat ttactcacgg aacgtatcct gcaatgccgc gacgagggta
    27661 ttcccttcca ccggctggcg gtcttatttc gctcctctgc ccactcgaac aaccttgaac
    27721 tgacgctagc acggcataag cttgccttcg tcaaatatgg tggtctccag ctaaccgaaa
    27781 cagcacactt caaagacgtg ctcgcgtatc tccgcgcggc agacaatcct ctcgacagca
    27841 atgcctggca tcggctactc ctgctcctcg atcgggtcgg gccgaagctc gccgagcggc
    27901 tcatcgccac catgcaggag gccccccacc ctttggacgt gctcgatcat gtccctggca
    27961 aggcggaatc aactatccgg cttttaaata acgttatccg gacgacacag gacgatacga
    28021 tttctttagc cgatcgtctc accttcgtaa tcaaacacta cgagccgatt cttaaacgcg
    28081 attatccaga agatgccaaa gtccggctcg aagatctgaa gcaactctgc tatctcgcgc
    28141 ggcaatcact caccttagaa gcattcttgg aagacctcac gttgaaccct tcgaccacac
    28201 cgggtaccaa gcatgcccac ccagaacacc acctcgttct ctcgacaatc catagcgcca
    28261 aaggtttgga gtgggatgtg gtctttgtta tctgggtcct cgatgggcag tttcctcccg
    28321 tcagaagttg gcatgacgag gacgcccttg aagaagaacg acgcctcctc tacgtagcta
    28381 ttacgcgacc gcggaaccag ctcttcctca catatccgtc agagcactat cacaacgcat
    28441 ctcagcagaa ccttgactcg ctctgtcggt ttctcgcgcc gatcccagac gacattcttc
    28501 ctggattcgc gatggatcaa gtctgatgaa gtctccctgc atattccgtt tcgagttccg
    28561 cacccaatgc gaacggaagg atcgcccctt tgtggcacgc gtgacatggg acaccgagac
    28621 caaatctctg gcacgtggtt tttatgccat tcaaatcacc ccagtaggcc gttttggagc
    28681 cgtcggtgaa tgcaccttcg atgccgcggt cggggacatt gttgaagtga aacgcggtga
    28741 aacaaaagac cgggaatatc gcggcttcta tctcgtcgtc cccaagggat acttgatcct
    28801 cgtcgcgatt gccgatgagg tcgatgacgc cctcaaagtc gtccaatacg tggccgggca
    28861 ggcttcacct caagacctcg caaagggaca ttggcggttc gaatctgccg caagctcgtg
    28921 gaagggcaga acggcgcccg tcgatcagcc acgaatggaa ccacatcccc cgcgacctgt
    28981 ggcgcagttg ccaccgaacg agaagcgaca gactaaagtc acgggagagc cagcttccac
    29041 cgcacctcga agcaacagca ctcgttcctt cacacgggtt cgcagacaca caccccagcc
    29101 aagcaaggat gagaaggaat gacgttccac atcgccagtt tccatgtacg gctccactac
    29161 acatggctca tcggatttgg cctcatcacc tggagtctcg cagctaatta ctttcacgac
    29221 acgcaggctg acctccccgt cagctaccag tgggcgtccg gagcaatcgc caccctcctc
    29281 attctcgtct cagttttttt gcatgaactg gcccatgccg tcaccgcccg gcgactcggg
    29341 tacgccgtca agaccatcga attgcacatt ctcggaggct ggacaatttt tgagcgggaa
    29401 ctctcaagcc ctcgggtgga aattctggtc gccctcgcag gtcctggttg ctcctttctc
    29461 cttaccattc tcctctatgc gctgaaggca gaccctctgg caaacttcct ctacaaagtt
    29521 aatctcacac tcggcgccta caatctcttc attccctgcc tgccaatgga cggcggccgc
    29581 atcttacggg cagtcttgtg gactcaaacc cattcctttg cggtcgccac tgaacgtacc
    29641 gctcaaatga gcaaacaaat cagcagcggc atgatggcca tcggcattct cggcataatc
    29701 tttcagtacc aaacgctatg gcttgcgatt atcggcggca ttctccgcat gatcgccgag
    29761 accgcttatc gcaccgtaca acagagtgaa cagctcacca ggccgctgca ggaagtgatg
    29821 attccgcggg atcgcatggt gacgctggcc cacgatacgc ccctacaaga gctgcaagac
    29881 cagtttcttc gttacgggta ccgagtttac cccaccgccg aacacgggca gatcacaggc
    29941 ttggtgtatt acaaagacgt gcgcaccgac cccaagtggc tcgctccgta cggaacgacc
    30001 atcaccccgt tcattcgcaa cttgtcctca gatctaatcg tgcacccggt tcacacttta
    30061 caacgggcgc tcgatgcgat gctcctcagc ggttcggatc agctgttggt gtttgcgggt
    30121 gggacgtgtg tcggaatggt gacacgctcg atgatcgccc gagtccaaga cgcaatcggc
    30181 gatccggact cgggccctac gcttacgcgg cagagggagg ggtttccgat ttagggctat
    30241 tgaccgcagg tgtaggaaac ttcacctgga cgtaggacgc actgctatcc agcgtcttcc
    30301 ctttgtacca ctggccatga gtacgcatgt aaaaacaccg ctttggcaaa tgcaacacgc
    30361 ggtcccgcaa caccaagcgt tcttcattca aatgcaacgt gacgttgcct tgcacagaca
    30421 cgaccgaccg tggccgttgc accagcggag aagcatcctc aacactttgc gccgtctcag
    30481 catgattgac tcgaaaatag atccagctgt tgatattgtc gctgatactt cgggccagat
    30541 ccgccccaat ctcagccgac atttgcgcac ctgattgctc atagaagtgc agccaaacgt
    30601 ttgccccgcc ggccttattg aaaatttctt gaatccccgg atacagcacg ttatgtcctt
    30661 cgtcgatatg cacgcacaac ggaggatcca actgacggcc ggacgcgaaa agacgtccaa
    30721 cgagactttg aatcatagac acaaacaccc gtccgaagat gagggacgcg cgaggactca
    30781 acaatgaccc agtatgacat accaagatga cccgcttgcc ctgctccaga cgagtgatga
    30841 attcattggt tcgcgccttc ccaataatcc gaccaatatt cccaaaagtc agcgcgctca
    30901 gcatcgtgcg aagggacgac gacaccttgg cgaaatgctc cgcgatgccg ggaccatggc
    30961 acactctatc aatcaagcca cacagctcct ccgtatcatt cagaaccttt aagacatctc
    31021 ggaaattgat caattcagtg tagcccaccc gttgattgac atcgttgaag gagatggtcg
    31081 ggacttgccc tcgacttgtc gcgagtttga ttagtcccga gacgatcacc tgggaaacct
    31141 cagacgcaat ggagaggtag tagtcatcct taaccttgat ccctgagacg atatggctga
    31201 ccaactcatc ttccatatag taattcgcca acggatcgag atagttggag tagtctggaa
    31261 agatcggcgt caccagcatc acttcatcca gcctcccggc ctctgcagcg acctgcacta
    31321 cctttgaaaa gaggtcgatg tcccctttgg gatcgattgc aaccaccgat ttgtgactcc
    31381 ggatgtcctg ctcaaggatg gactccatca atcgcgtttt cccgatacgg gtcgtcccaa
    31441 aaatccccac atgcccagaa cgatcctcat ctcggatcac aaggtatcct tctcttcgct
    31501 gagggtcctg caaacatgcg ccatatccca ccaagacctt cccgtccaca atgggatctc
    31561 gctgccctgc tcgattacgg atcctcaatt cctcaatgcc ttgttccgac atggctcccc
    31621 ccttctcatc gtgtattacc agaccattgt ctcagccata cacaggagta ggtcatatcc
    31681 aagctccttc tgccgcccac accctcactc atttagacat cagtaataca aagatatcga
    31741 ctaagtcgaa atcagaacaa cccggagagc catggagatc acgacaaaaa tgttggccca
    31801 gcccagagat aaccaagatg ccgatagaca cgccctcacc cccgagccgc gccagctcgt
    31861 aacggccaga ggaacacatc ggaacctgcc gaatcgccgg acaggctata cccagaaagc
    31921 cacgataggc gggcacaagc tctatatcag gacaggagag tatgaagacg gaactctggg
    31981 ggaaatcttc ctggacatgc acaaagaaga tgccgcattt cgtagtctca tgaactgctt
    32041 tgcaatcgcc atatccttag gactgcaata cggcgttccg ctcgaagaat tcgttgaatc
    32101 cttcacgtta atgcgcttcg aacctaacgg accggtatcg ggacacgatc aaatcaagat
    32161 ggcctcgtcc atcctcgact acatcatgcg tgatctggcg atcaactacc tcgacagaca
    32221 ggacctcgct catgtccagg ttactcatga cgacctccgg ggagactccg tcaaacccta
    32281 tgccaaatcc acaaaccacc aaacaaggca agaatcttca tcgctcaaca ccatggcctc
    32341 gcacccaaca aacgacattg aagaagcccg aatcaaaggc tacgagggcg acccgtgccc
    32401 agagtgtcat cgattcactc tcgtccgacg gggcacgtgt cgaacatgct caacatgcgg
    32461 gagctccacg ggttgcagct gaacctagct aagacagtcc ctttactcag atcacacgga
    32521 gtgtctatgt cagtcccact gcccacccct ccacacaaca tctggctcca acggttcaaa
    32581 cgccttgctc agttgaccgc ctcgctcaat tccgaagacc cccgcttccc catcatcatg
    32641 acactactcg ataagtgtga tcgcgactac cggacagaca accatgcctc gtttatcgag
    32701 accggcaaac gcattcgttc cctcatggac accccaatca ccagctaata cctaccccag
    32761 aaacgaccgc gccaccagcg tgcctctaac accatttcat ggatcacgac ctcttaccct
    32821 ccgaaaccaa aaggatcccc atgtcagaac aatacccgct ggacatcgat tttcgtactg
    32881 ccagaaacgt cagtgtgccg cttattgccg tcaactcact ggacccagcc gccaccatgc
    32941 gagcccttgc ctggtcattg gtcaatgtcg caagcgcctc ccaagctgat accgacaacg
    33001 aattcggcct ccatgctttc ccaatactaa aatgggatat tgggcatggc ctgatcccgc
    33061 tcaatgagga gggtaagaac tggcataccc aaaatgtcgg gccctccaaa caaaatacga
    33121 ccatcaaccc tgcagaagcc ctccgcgtcc tcgaaaaggt gccgcaacgg acactgacat
    33181 tcatcctgaa cggacaccgc tacctctcaa acgatttctt cattcaaggc ctctggaatt
    33241 gccgcgacct ctacaagacg aacggaagta cgatcgtcct cctgggtatc ggatttaaac
    33301 tgccacccga gctcgccaat gacgtggtgc tcctaaacga gccgctcccg acggccacac
    33361 agctggaatc cattatcaaa gagcagctct ctgcagcatc gctgccatta cccgatcctg
    33421 ccacattggc caaggccgtc gatgccacgc ttggccttgg ggccttcacc gccgaacagg
    33481 aagtcgctcg gtccatgcaa gcgagcggac ttaacgtgga caaattgtgg gaacgcaagc
    33541 gacagatcat cgatgcgaca ccaggactca gcgtctggcg aggagggagc agctacgcgc
    33601 aaatcggggg agtcgctacc atcaaacact tcctcaaaca ggttttggtc tccaaacgcg
    33661 ctccccgctg catcgtctgg ctagacgaga ttgaaaaaag cctctccgga tccactggac
    33721 aagcctcaga tacatcaggc gtctctcagg ccttactcgg cattcttctg agctacatgc
    33781 aggaccacca ggcctcaggt ttactcctcg tcggccccaa cggcacctcg aagagtgcca
    33841 ttgcgaaagc cacgggagcc gaggcgaaca tccccaccat cgccttagac atctctggtc
    33901 tcaagtcctc actggtaggc tcaagtgaac aacagatgcg gcaagcgctg aatgtcatct
    33961 cagctatctc tcaggacaaa gcctgcttta tcgccacgtc caacaacatc agtcagctac
    34021 cgcccgaact catccgtcgg ttcggctatg gcacctggta tgtggatctc cccagtcagg
    34081 atgagcgcga ggcgatctgg aacatctact tcgccaaatt cggtctcgcc gccgacacag
    34141 accgaccgaa tgatcacaac tggacaggcg cagaaatcga gcgctgcgct cggctcagtt
    34201 gggaactctc tatgccactg tccgagacag ccaagtacat cgttcccacg gccatcagtg
    34261 caaaggaaag catcaaggca ctcgaagcac aggcgcatca gacttatctg agtgcgaacc
    34321 gtgagggcgt ttttgaccag aaccgagaca cgccgcatca cacacgtcct cgcaccatta
    34381 cactggcgca ataagccagc gctgtcgcct agagaaaagg aacacgccat gccctgctat
    34441 ctcgaacaaa gagtctcggt ggagttgaat atggccgatg tccccctcct tatctccgcg
    34501 ggatcttctc tcgggtggga acacactctc aaagacgacg tcctggtctt caccactcca
    34561 caaaatcagc aagtctccat ttcgaaagga agagccacgc tggaccagcg acacgaagcc
    34621 ctgattctgg agctcaagca agcctatgca caaaccatca ttcagacggc cgctgaacaa
    34681 ttcgggtgga cgttagaacg cgttgagggg gcagaacaag aattactgct cactcgtttc
    34741 tcctaacccg gctggagtga cctttccatg cagaccgaca gcatacgcat caagattgaa
    34801 acagatggaa ccatcaaact gattaccgac gagatcagcc tagccaacca cgccactgca
    34861 gaagacgtta tacaacagat catcacactc tcaggcggct cactccaaag cagtcggcgc
    34921 tcaacacaca atcaccacca tcatcaggcc gacgaacatc ttcaccacta agcgaggtca
    34981 tcatgctcaa tgctcaatcg gcatttagtt cactgtggtc caacgcattc tgcctcatgc
    35041 tcacgtatcg caaactcggc attcaccgac ggatggacag cgccacggtg ctctccattg
    35101 atacggaacc cagtcgtgtc cgtgtgacta aatctattct gtcctgtccg gaatacctgg
    35161 ccattacaag actgtactcc cgcgtccgca ccaaattgga aaagttgact ctgccggccg
    35221 gccttcgttc aggcatgtac cttattcctg tctcgctcgc ctcagaggtc gacaccatta
    35281 ttgcaaacgc agaagacgag ctacgacaac tcgttgatgt ctttctcgcg caatacgata
    35341 aacgtattgt ggaagaggag tctcgcctca aggcggccta tcacaccaga gattaccccg
    35401 atccagacac tgtgcgggcg gcgttcggac tcacctactc ctatattacg tttgatcttc
    35461 ccggcaccct agagactatc agtgccacga tgcttaaacg agaagaacgc cggtcaatgg
    35521 aaaaggtgaa caatatgctc gaagacgtaa atacctacct ccgagaagcc atgcgctcgc
    35581 tcgttaacca cctggtcgat cggttaagtc cgagtgaatc tggcaagtcc aagaccttca
    35641 agagcagcac gctcaccaat ctcctggaat tcctgaacac cttcgacgcg cgcaatttgg
    35701 gtaatgatat cgagctctct caactcgtca caacggccaa aaccgtgctg agtggagtag
    35761 actcgcaatt gctccgcagt gatcaatcga tcgctaacgt cgtgcaaaac gggatgggta
    35821 cgattaaggc ccaactggac tccctcctca tcaagaagcc gatacgcctc tacagcttcg
    35881 acgaagcggc ataaccttac gaactagacc cctcaccact acacaccctc tttctcggta
    35941 ctgataacgc acatgtccat ttctccagac gccatcgccc acgcccgcag tatcaacctc
    36001 attgagtttc tcatctctcg gggccacaaa ccggtgtcga gaaggccgca ccatgcagat
    36061 tttcacgccc cttatcgtga agatagaaat ccttcgttct ctgtctcgca gcgaccggat
    36121 ggcacctggg tgtggtatga cttcgggcgc aacgaacatg aggggtgcaa acagcacgga
    36181 gacctcatcg acctcgtcca actcctcgac catgtgccgt tcgcagaagc cctgcaccgg
    36241 attctgcaca acaccgtcag gagtatggtg gctccgagca acccctccac ggccccccca
    36301 accgacaaag agcgtattgc tcacgccaag aagctctact atgccgccca agccaacatg
    36361 accccggaac gtgaggaaga gctccggtcg tatttccata aattggcgct cccctacaac
    36421 cagcatcttg gcgctgtatg gatgcggcta ggcgacgacg gcatcccata cgtagccttc
    36481 ccgcttccca cacccaacat ccatttcatg caagggctga tggcacgagc cttgggcgag
    36541 gccccacccg ccctgatgcg ggtcgcccgc ggcctcaagg gcccctggat ccttaaacgc
    36601 gggaacgcgc caattctgat cacggaaagc atcgtggatt gccttgcagg cgatgagcta
    36661 ttcggaccct cgtttaccct ctgtgccctg aatgggctga atacggttga gcgcctccag
    36721 gaatatctca aacggctccc ctcacgaatc atctatgtcg cgttagacaa cgacgcaagc
    36781 tgtatcgccg tgggaaatgc gcttcagaac agctcacgaa agaaggggcc acacgtccag
    36841 aaagaactgg tctcgctgct gactcacatg ggctatcacg ttatggaagt gctactccat
    36901 cacaacgcca gcgtaaagga tcttcacaag ctttggctga agcatccgca gcgagtgtcg
    36961 ctgctcgatt tggcaaaaac gggcctccac catgcaccag cgtgctagcg cagccgcgtc
    37021 actttccatg gcctcgtccg tgacctcgga caccccgtca cactcacggt atgacaccca
    37081 ctactacgac cacaggacct aaatgaccac ggcgtgacct acctggtaga cactgtgtcg
    37141 atgaagccct agatagggcc gcactccgga attcccaaca gcgtcaacct attcaagtca
    37201 cagttgcctg cgtctgtcga aaaggagcgg tatgttcagc gaagatttct accccacgcc
    37261 cggggccgtc gcagcgaaaa tgcttcagaa aattgatcgc aatgctgtcc attttcttga
    37321 gccgagtgcc ggcaaaggcg atctcgctaa agccatattg ggattcggtc ggactcggtc
    37381 tccctacgat cacggttcac gccatcgcgt cgacgtgatc gaactccagc cagatctctt
    37441 gaagattctc caagccaatg aggagctaac cgtcgtcgga tacgattggt tgacctacga
    37501 cggcgtctcc tattacgacg ccattgtgat gaatcctccc ttcagcaaag gtgccctcca
    37561 tctactccgc gcttgggatt ttctgcacaa cggcgaaatc gtctgcctcc tgaatcaaga
    37621 gaccatcgat aatccttaca cggaagaccg tcaacggctg gccgcgatta ttgctgcaca
    37681 tggctcggtc gaacctcttg gtccctgctt cctcacggcg gaacggccca ccgatactca
    37741 ggtcgccctc gtctacctta agaagactac ggaagatgat cgcatccatc tttggcattc
    37801 ggccgaccgc gaacaatctg ttaacgatga catcggcaca cctgaagcga tccccgcgct
    37861 tcgcgatacc cttggaaata tgcagcacta ctacacacaa tccctagaag agatgtttaa
    37921 ggccttcgcc cacatccgca aagccagtct ctttatgggc gcactcggca cggagcttcg
    37981 cccgagccga tcgacgcgag acgagtccga tcttaaaaag atccttggct tggcccaaac
    38041 caacaccacc gccgcgcgcg ccgaattcgc acgggccctt cgccgaagcg cctggatgca
    38101 cgtctttgaa caaatggact tccggaagtg gctagatacg aaacagacag aagaactctt
    38161 acaagacctt gaacgcgata gcaccgtgcc ctttaccaca cagaacatca aaggcaccct
    38221 ctccaatata ttccaacagc gcaaacgctt gtttgagaaa tcggtatgga atgtgttcct
    38281 cacgctcaca cgacactaca agggcaacac caccggagac atcgggagcg gggacggcaa
    38341 ggcgggctgg aagagcaacg actcgtacaa ggtcaattgc cggcttgtgt ttccatacgg
    38401 ctgtcgattt tggatcggaa gattcgatct ctggtctgcc cgtgacgccg gcgagattta
    38461 ctccgatctg gatcgcgtcc tcgccgtgct agatggacaa tcattcgaag agattgtcac
    38521 ggtgcgagac gcgctggaac gctcatttca caatcatggg gtgcaccccc acccctgtga
    38581 gagtaccttt tttcgtatac gctatttcaa aaagggaacc gtgcatctca aatggaaacg
    38641 agaggacctg ctggccacct tcaacacgac cgcagccgca ggacgtcagt ggattgggac
    38701 cacttgccat agagatcagg actcccaagc ttcatattct catccagaca gtaacgaccc
    38761 ccacagctgg ccatcgcccg taccaacatg ccagatttgc gcctagagag tagccccaga
    38821 ctccaaggct aaaggccgat cggccttgga tatgttgcca gcggtctcgt caataacacc
    38881 attcttcaac agcacaacaa gcgctgggca atcaagggca ccacaacaaa gacacgcgtc
    38941 gatctcgaac cgatcgtcga agtcacagac agagcaaatg aacgggagga cagaacaacg
    39001 gagaggacaa tagagaggtt tacacccgta atccagggat gggacctcac cccgggtgag
    39061 agcttcggcc agcacctcat catcgactgt gaagcgccac ctcccaagcc cgaaagcaaa
    39121 gccgggtagt tacttctgaa tccaatcggt cccggtcaga ccatgcttga tcgcgaactt
    39181 ggttaggtcg gcatccgtct caatgccaaa tttttccctg aacatcgcct tgtggtgttc
    39241 tacggtcttt cgactcagac ccaattgaaa cgcggtttct ttgatcgtct tgcctgaggc
    39301 cagcaacgtc acgacttcac gctgccgctg cgacatcccc aaggaggcga gttgatcctc
    39361 ttcgtcatca gaaccaggca gagcccctgt cggacgacta ccaagcaggc tcgtcacata
    39421 cgcgttgcct ctcatcactt catcgatcgc atggcagaac tcctgagcgc tgcaactctt
    39481 catcagatac ccattcgccc ccacggataa cgctcgagtc atgtattcag gttctttgtg
    39541 catggtcacg acgatgatct taatgtcagg cctgatttct cggaccttcg gcactgcatc
    39601 aaggccagac atatccggca tcgagatatc gacaaatgcc aaatccgcag gaacccgctt
    39661 cacgagttcg agcaatccac ggcctgaaga ggcttggccc accacggcga actcatagtc
    39721 acgctgctta tggttaactc catgtaagat ggtagtcatc ccatccgcga ccaacgcatg
    39781 gtcgtcagca aagaccacgc taatcgtttt taccggagag ttgatcggag aattcacact
    39841 ggtcgtcatg cttcacgctc cctataaggt gcccgagcca ctacgacagc tcctcgccct
    39901 ttccctgact gaaccgccaa ttcccctgcc accatgtgga tgcgctcttc catcgcgaca
    39961 atccctagtc ccttggttcg acgcgattct gtctctggac tgaaccccat tccatcatct
    40021 ttcacactca gttgcaccca accctcttct cgagccaagg ataccatgac ctgttgcgca
    40081 ccagcgtgtt tcttcacgtt catcaaggct tcctgagcaa tccgatacag acaggtcgcg
    40141 acctccctat ccaaggcctc agtaaactcc atcgatatat aactgacgat cttcatcccc
    40201 gaccaactgc catactcctt caacattttc ttgagcgcca ccgagagccc aagattatcc
    40261 aagatcgatg ggtgcaactc gtgcgcaatc gttcgcacac gatcactgat tccccgtagc
    40321 tcgtcagtga catttttgat gaccgccgcc tcagggctcc tgttctccaa caacaccaat
    40381 ttcccttcgg ccctcaaaat cacacttccc aatcgtgatc ccagatcatc atgcaactcc
    40441 ctggcaaccc tgcgtcgttc gttctcttgc gcggtcagca actgtgaatg gagggccctc
    40501 aacgcttctt ggctgcgacg cagggcctca gctccctcct gaataacact ttcggcacgc
    40561 tttcgctctg acacgtctcg cacgatggca cacacatacc cttccccatc atgctccaag
    40621 tagcgcatcg acacttcgat gggaaagacc gttccagact ttcttcggaa tgtagtcgca
    40681 tactgtacta cttcgccctg tttcagccgc gtcagatcgt taagaatgtc gattcggctc
    40741 tgtaaaggag caatatccgg catcgacaaa ttcaagagtt cttcgcgtga atagcccaac
    40801 aactcggttg cccgtacatt cacatacatg atgcgacgat caggcccagc ccaaagaatg
    40861 gcatcagccg catggtccgt cgcaaactgc gtaaaccgca gcttggcttc agccaaccgt
    40921 cgctcagtca catcggttaa caaaccgtga aacagtactg ttccgtctac cgtaggcctc
    40981 tcaggcagag aatccccacg caaccatttc acgcgaccat cggggagtcg taacctaaac
    41041 tcatgcgccc accgcgatcc actgcggatt gcgttttcaa tggatgccat gatccccggc
    41101 atatcttctg gtaggaccaa tttccagacc gcactataat ctgacacaat gacgtctgat
    41161 ggatacccca ccatatccat ggctccacga ctgatatagc taaaccgctg acatccatcc
    41221 cgcgtaataa catattgata caccgcaccg ggcacagcat ccgcaatccg tcgcatcttg
    41281 aggtcagtct ggtgtaactc ttggacatcc tcgaaatact tcttctcctg atcgaccacg
    41341 agcgactcat tcagcttcaa gagccggcga taagtctctt cagcaaatgc gaatccaacc
    41401 tctctggagt acggcttccc ttcaccactc ttagccttag cactccaata ctgctcgtcc
    41461 ccagtagaac acgccagaca aatattgcct gataacactt gcaacaacga tctgatccta
    41521 gaatcgactg tcaccctcga gtagaggaag acacagtagc tccctccaga aggcagcagc
    41581 ccacccaggc aaataacctg tttcactcca tgcctccgca gaaagtctgc tctgctttcg
    41641 gcgaggttat aagagcgtgg ggtctcatgc accaggattc caaacaattg attgtgtgaa
    41701 ttagcgacag taagactctt ctcaaaatta ttcaccggac tgctcagccc caaatcctcc
    41761 cacgattgcg tgtaccacgc agtttcctgg gaactgaact tcgtttgaat ggggacacaa
    41821 tgtagtccga acggaggtct gtcgatcgtc cactccgcag aatctccggc cacgcccatc
    41881 aatgcaagga ctcgcgtttg agcgggggcg tggtccccat caaccgaaga cacccatcgg
    41941 tcttggtagc gcgctgggag atcaccaaga ttacgagtgt gaaagacctc gacgagagac
    42001 aattgagact gactactctc ctgggaacag aaagaccggg ccagagacat cgcaatgagt
    42061 tgacagacct cctcggaaga ttttccaact gtctctaaat ctcgtaccca cgagcctaaa
    42121 cgacacgcat caccgaggga gagctctcgg agatcaaacg tcgattcttg gcctttgacg
    42181 ctcaacgaca tactaccctc caactccctc tcaccaactc acaactcagt aggccgctct
    42241 ccgattcact ctgccgtcag agaatcgctc gtggacgtca cgccaactct atgcacatta
    42301 cacgtattac gacaactcgt cccaagcaca ggcggcagta tgacaaaaac acgctctgtg
    42361 actcaatacc cagctttcct gcgcatggtt ttttccatac acacactcct ttcggtctga
    42421 ttgtttcaaa ttggcacatg gcaactagtt cggtctcatc cactctgtga cgaccacgca
    42481 aacgggtatc gccaaggacc atccactgcc atccagtaat acatagcaga agcggttcac
    42541 actcgttcac atacccagga ggaaggcatg tcacgtatta accgtcgcac ccactgttca
    42601 gtcggcagaa ttctcgccag ccttagtgtc gttatcgcca caggatcgat cgttgggtgt
    42661 gctggccaca tgacgctaag tcatgaggtg aatctcacaa gcagtaacgc catctttctc
    42721 accccgtcgg aagagaagac aatctatctc cagaatcgca acaccagcga caactccaat
    42781 atcaactttg ctgaactccc gaacaagatc aaagcaaagg gatatgcaat cgttgataat
    42841 cccgacaaag ctcagtacat cctccagagc caggtagtac tctgcaataa attaaagccc
    42901 gggcaaaccg tcgatgcctt gatcgccggc gggttcggtg gggccatcgg agtcgcagct
    42961 ggctcggccg cagcactcag cggggcgtcg cttagccaga ttccaattct cggggcggta
    43021 ggagcggtcg ggggatttgc cgctagcaaa ttctcagagg actctatcgt cgcctgcgtg
    43081 gtcgacgcga tggtacagga gcgcaccaag gaagaaattc aacaatctgt cacgaccaat
    43141 agtatgccag ggccaggcac accacagtca caacctggag cttttggctt tctgaatcaa
    43201 caaagtgtct ccccgcctca agctggacag gtcacccaac agatcagcga aatccgaaaa
    43261 ggcactcgcc gctttcacca agcgagaatc gtggcgtctg accaaaagat gtggctgtcc
    43321 gttccggaag ccagcaagat actcagtgac aaacttgagg attcactggc aggcctgttc
    43381 taactctacg gtttgctgac aagacttgct ccgtcccagc gaggcgcatc cggcgcctcg
    43441 ctcaacgagc agccacacct gatctggaga ccatgctgct tttcaccatc attatgctct
    43501 ttctactagc tctcattacg gtcacatcca agcggtacgg tttccgagaa acttatctca
    43561 tcgcagctgg ctcagccgct ggcatgctga cgtcgtttat gatggcactg atcgtcgcca
    43621 atattcggca aaccaacgaa tggcacagct acccatttga atttttcatg gatgacccca
    43681 tacttgccta ctggaccagt cttttcacct ggacgacgat gtggattgtc atcctctaca
    43741 aacggaccat ttctctcgaa ccatcggact cttcgtcgtg acgcgtctcg cgcgctcgat
    43801 cccaccttag tccctgcagc cgagatggga ggcaatcaga accatgctta tttcactttg
    43861 tctgagatcg cccagggaag cccgagcctt gcggcatcac gccgaatgag actaaggaga
    43921 taacttccga cgttgagatt ctgacctgac aaacgacgtt cctttacttc tccaatacgt
    43981 cgccagatca tcggatgtgg cacgctggca atcgcgcgct tccaccattt ctcccacacc
    44041 tttgggtcct tccgtcggcc ggacaactct agcacgatgc tcaatagcct cgcgttcgaa
    44101 gcttctcgtt cctcatcctt ctcgggactg ctctcatccg attgctcaat gaggaggaac
    44161 gggtcgagct ctcgtccgag ctgcggcaat tcacgctgac tggatccgac catctcttca
    44221 cggaattctc gcgtcttttt atccgtcgcc caccaggctg gccccgcctc ccaaataatc
    44281 tcaaactcct tcccctcagg cgttcgaatc ggcacatatt cccagcgcga aataaattcc
    44341 ttttcgagca actcacgatg agcctcatct aattgctgct ttaccagcga cagggcactg
    44401 tgtcgcttga tctgccaatt ccggaccagt tcggaatact tttgtgaagc cgttccacgc
    44461 ccaaggtaaa acaggttatg caacagttga tgcagcagtt tggtgattga tccatgaaga
    44521 tctcgaaaga gttcatgatc gatgacgata caattgccag accgcaggct catccaatac
    44581 cacgtggcaa gctcaacctc gtacttatct gccacaaagc catccgcatt gacttgcccc
    44641 ggcaacacta ccgtttgaaa gatatgaaaa acgattccct tattcgatcg aggacgcttc
    44701 tccgttcctc ctgattcgcc ttcctgtcgc caatcccgcc cggcgccaat catcgtcccg
    44761 gccatgcgta ggaagaatcg atccatttcc tgatacgaag ccccgccaag acctttcccg
    44821 ctcgcctcca acaattcgcg ccctgagatc ttgactaact gattaaacgg ctcgccgcgg
    44881 ctcatcgccg ctttcgtcca ccgttcaaac ccccgaaaga actcgagatc gattgaattt
    44941 ggaagcccca aatcgccgct accgtagacc accaacgaga ctttgcacag acggccgttg
    45001 cgccacacat ctctatagat ctgcttctgg taagaggctc gtttcgctcc cgtcggccga
    45061 cctttggctt cccacagcgg aatcgatccc aagatatgct cgctgaggag atgatctgga
    45121 gtggtaacgg acaaggagcc tggatcggta agagtgccgg gaaagactaa ctcagcggag
    45181 atcccgacag gggaaagagt ctcttcatcc agttccgaaa gcggtgttaa atcaacgtcc
    45241 atcagcgtaa cgttatgtat taattctttc gtaagattct aacgtagagg cacgatctgt
    45301 caggcgcttg cttcttgctt tgcatagcac aatcaatgtc ttacaaaaac tgtgtcgttc
    45361 atcgaagtag cgtgacttca ttcaagttac gtcgctcttc actcaactag cgaaggaatt
    45421 gagccgtttt cttcactcaa ctagcgtgag cttcaccgaa ttgacgcgaa atcttcattc
    45481 aagtagcgat ggggctgtgg gtaactcact tctcgtaccc tctcatcgct cgtgctcgat
    45541 ccggtaatac tataaatctt taaaaatgct gaaatatttc gggctgaaaa aaaccattgc
    45601 caacgttgtt cagcatgccg cccgtctgtt cgtaagttga atgaaacttt tcaccgaaga
    45661 tcccaggcta ggtcaacttg tcacgaaact cctggatgcg ctccgacgcc tgttttaagg
    45721 cttcttcaat cattgggatt tcatgcggct tgttggaatg aaagagaatg gtcagcatga
    45781 acccacggtc tccacccctc ccacgctcca cgaaccgcac ggcttgggcg ccttcattaa
    45841 ttcttggttt gcgatcgatt tcctgtcgga tgcgatctcg agtccacgat tcttttgagg
    45901 cacggcgagc gatcgcgtgg aggcgtggct cgtcatgagc cagagggagt aatgcctctg
    45961 cgtgggccgc cgtcatgtga ctttcacgtc caaagaacac gttcaagata gtctctggca
    46021 atttcaggat gctgagcgcc tttgaaatgc gtgatttatg acagcccagg cgttcggcta
    46081 actcggactg ctgaaaacca taacgttcga tcagggcttg ataggccttt gcttcctcta
    46141 gggccgacag gtccttccgg acaacgttgt cgatcaacgc ctcggcggcg gcgcggtctc
    46201 ggtccttccg acgtaccaga gccggaatcg aagtcttgcc gagcaaggca caggcccgcc
    46261 atcgccgctc gccggccacc acttcat
//
LOCUS       DAORRQ010000035        44391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_51_length_44391_cov_56.115527, whole genome shotgun sequence.
ACCESSION   DAORRQ010000035 DAORRQ010000000
VERSION     DAORRQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 44391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51_length_44391_cov_56.115527"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..695)
                     /locus_tag="PK224_12125"
     CDS             complement(66..695)
                     /locus_tag="PK224_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRB16530.1"
                     /translation="MKIVSPSRSGKRFQQIHQLALMVYTIMLLAGCVTPEPTSAQSSL
                     ADPGYQLGPEDVLMISVWKDEHLTKEAVVRPDGMISFPLVGDLPAAGHTVEDIRQDLA
                     KRLSRYVPNPHVSVAIIKLQSYKIYVMGRVNKPGEYLIGHYTDVLQALSLAGGLTPFA
                     AENDIKIIRRQSGDDGDQRVFYFRYGDIQKGRDLKQNILLQRGDVVMVP"
     gene            complement(707..3112)
                     /locus_tag="PK224_12130"
     CDS             complement(707..3112)
                     /locus_tag="PK224_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16531.1"
                     /translation="MGAIFQRCTVLGKLGVATLLILAVGCGGPEQRKAKYRTKAQEYM
                     QAGNFPKARVALRNVLKIDPKDSEAYFLYAQVEEKEKDWRHAFAHYLQVVELNPTHEQ
                     ALVKLGKFYLQGRLDEKARETADQLLRLYPGHVSARAIHGALLAAEGKLTEATAVGEA
                     LVKQFPTEPDAAILLGTLYSLQNAPHRVEAVLKKAVEAHPRDLDLLNSLAMAYMRVSN
                     QTKAEAVFQNILTIEPQVYDHHLKLALFFDQQRQYDKAEATLREALRLEPDSEQRHLA
                     LADYLAGRRNVAASEAALAEARHALPHAVNIQLALGRLYEMHQQPDKARATYQDLRDR
                     NQSKPAGDTAKVKLATLDWVEGKQDAATEQLQEVLRNNPRASEALMLQGRIALQHGAG
                     REAVQAFRTVLKDQPEKSEAYALLGQAHLMLEETNLARENLEKAVALSPTLYQAHLAL
                     AILDASSGRTRDARLRLEELLKQAPTNLGTLGMLLNLQAADRDWDGSDQTLSRIRAAG
                     ANRFVSDLAEGNLYQARLQWDKAIAAFERAAAQNPDAPEPTFALVRIDTRQGKLAQAQ
                     NRLANLIAKNPRHQFAHGLLGEILILKGDPAGAEAEFRLATGLKPEWSTPWVNWVTLK
                     LSQRKGEEANEILQHGLQVNPKNEELRLLYASHLGDRGQFDLAITEYETILSQNPRDL
                     MAANNLAAILTDQKGDTKSLDRALALSRDFEQRAPNPFFLDTLGWVHLKMGHQDDALR
                     VIQRAVAQAPQHPLLNYHLGMAHFKAGHRLEAQAHLKKALNSKQPFPGMDEAKAVLAE
                     VTG"
     gene            complement(3130..4710)
                     /locus_tag="PK224_12135"
     CDS             complement(3130..4710)
                     /locus_tag="PK224_12135"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRB16532.1"
                     /translation="MALIPPLMATASRLHFLDIPKDQRRVHSNPVAKVGGIAFGIGTF
                     AAMLIWAPKDDIVVAGLLGGVTILLFGMWDDRVGLPYSIKFLGQILGALIVIWQGDIH
                     LNALPFVDSELPLWIAYPLTILIIVGVTNAVNLADGLDGLAGGLSLLSFAGMACLAYL
                     SGDHTVTLLMVPVLGGLLGFLRFNTYPARIFMGDAGSQFLGFFMAVSAIVLTDPQRGP
                     YTPPLGLLLLGLPLLDTIGVMLQRLKEGRSPFVADKNHLHHKLLAMGLSHYEAVLMIY
                     FLQAMLVTLAYLLRWQTELAILGTYCFVSALILFPFLRSRAWNIQSNRLLKRADRFLT
                     RTSTITSSPWLTTEPIRLLGIAVPAFLIASVFLPKRVPDDMAYVASILFAVLLFGLWL
                     SPRTSTLVVRGGLYVGSTFIMYLSEQPAASTDILSPIHVFFFGLALLVTLTIRFNSEH
                     RFQTTPLDYLMVLLAVMVPALPEMQIGDIHFSFLTAKLIVLFFSFELLLHVFAQQVTQ
                     LGLVALWLLFGIAIRAWL"
     gene            complement(4782..6332)
                     /gene="xrtD"
                     /locus_tag="PK224_12140"
     CDS             complement(4782..6332)
                     /gene="xrtD"
                     /locus_tag="PK224_12140"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HRB16533.1"
                     /translation="MIAAVLLPLGILAFVYYESLIPLVKEWIGNDTYSHGLLVPLVSA
                     FLIWQQRTELAERANGGSWWGLLIIASGLFLYIVGELATLFILIHLSLWCILIGLLVA
                     ALGMRAVASISFPLGYLLTAIPLPQFLYQGLSGHLQLMSSALGVGCLQLVGVTAFREG
                     NVIDLGPIQLQVVEACSGLRYLFPLTSLALLCAYLLQDRLWKRIVLFASSIPISILLN
                     GFRIAMIGVLVDHFGEAAAEGFFHAFEGWAFFMVSFGILLVEMWILRKIGPCCRTDSR
                     GEGVASKPSLLSTTGTHGFTAKLLPSSSLSPYVCSIGVLLLLPLISPQIIDRHEMVPT
                     RQPFLDFPMLVEGWSGSSYPLESQYIDALRFDDYLLADYRFGQSSPVTLYMAYYRSQR
                     KGQSVHSPQSCIPGGGWEILSVEPVRLGGPNVSLPLSANLVLIGKNDQRQVVLYWFKQ
                     RDRQLTNEYLVKLYLVWDAIVRQRTDGGLIRLASAIRPSETEEVSRQRVLALAEAVGP
                     RLQPYIPD"
     gene            complement(6497..8443)
                     /locus_tag="PK224_12145"
     CDS             complement(6497..8443)
                     /locus_tag="PK224_12145"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRB16534.1"
                     /translation="MISMKVSINMNALPPGLAWYAKRLSAMGPTGVMHRMRDAFVVQS
                     LHMRHRLSGSGTAQRYTAADIPRHYFCATSEPVLPHIPLQFQPTDEDASQLLSGRLPI
                     FGNEWTWHSQASAWHEAPDTKQQWPQVFFHRISCGSDNPYGDIRIAWEPSRLQHLVAL
                     ALYAQQAAPDHRRKAVEVIEAQLLSWIAANPPLVGIHYVSVMECAVRLIAVCHTVDLI
                     REWLTNPQEIWVGVLNLVGQHAELIRKRTTGYSTTAHHTIAEAVGLLYAGLLFPKMDM
                     AERWFAFGLYLLENEATRQIRQDGGGREESFCALRFVSDLYGLAIHLLQQFDRPVPNK
                     LEDAFARSSSFLQAMMRPVDGALPQIGEGDDGYALSPHLHFVSAPKAQSPGLTSFHLS
                     GYSLIHGQNRQRLLFNHGELGMAPRYSQGHADALAVTLEIGDREVLIDPGTYSVTQDT
                     TWRQYFRGTRAHNTVTINGQDQAIQETGFLWSQPYATHLIYREEAPNGTITILARHYG
                     YLERFGVSHWRGIVYQPQGAWLIWDWLTGTGTHHLELNWHLGVTPVPQPASHLFVVGE
                     ERVQCSVEGGQVSLHCGEENPIRGWHASHYGSRTPTTTLRAEYRGTLPHEFITRIWMG
                     EGAPPDSLQAFSLSGWSRGSSLRA"
     gene            complement(8869..9552)
                     /locus_tag="PK224_12150"
     CDS             complement(8869..9552)
                     /locus_tag="PK224_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16535.1"
                     /translation="MTNRFLTLSLSLFILSASSVLSLLHVSKADAALVTYQFSGNISE
                     VSGSLFTPGGQGANGFNAGLKLSGTYTFDSATAGMLPSGLGAQVYNASVTEMNLSIGN
                     YSAPLQGGGTNLTTMTSGSTSLYSVNLGTAGNLVKGLAPSLFSLNLADESGTAFSSVA
                     LPGNPAPNLNSFNVNRWTLVFSNGAQLVGSLDSLKAVPLPATALLFGAGLIGLVGLGA
                     RGRLSNPLS"
     gene            complement(9920..11152)
                     /locus_tag="PK224_12155"
     CDS             complement(9920..11152)
                     /locus_tag="PK224_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB16536.1"
                     /translation="MHILFLSHYFPPEVNAPASRTFEHCRQWVRDGHRVTVVTCAPNH
                     PRGKVYPGYRNRLYTREEKDGITIIRVWTYVTANEGFLKRTLNYVSYMAAAIVAAPFI
                     QKADIVISTSPQFFNGLAGYVVSRMKRAPWVLEIRDLWPESILAVGAIKNRFIIHLLE
                     WLELFAYRQADRIVPVTDAFQRYIEGKGIPKEKITVIKNGADLTLYKPVTGTNRLAES
                     LGVQGKFVAAYLGTHGMAHHLETVLYAAQRLREYPDIVLILAGDGAERQRLAALRNEL
                     GLDNVLMLDQQPKEQMPWLWALSDVSLILLKKSDLFKTVIPSKIFESMAMEKPIILGV
                     EGESADIVRAADSGYCIEPENAEELADRLLQLRESEDLRRRFGHNGRIHVRAHYDRLV
                     LARRFEELLRTTCSSGGH"
     gene            complement(11190..12929)
                     /locus_tag="PK224_12160"
     CDS             complement(11190..12929)
                     /locus_tag="PK224_12160"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRB16537.1"
                     /translation="MNSWIVFDELFHDASLCNGMIHRIVRFAHTLRFLRPRQLGYLII
                     RRLLPVRHRTASHGAVRRRLLVGPTSDWITREAAAGTYEFCFLNIRKAMSRPNVDWAS
                     SDMSKLWRYNLHYFDYLQESGRTQDEKRTLISDWIAHNAPGATDAWEPYTVSLRLVNW
                     IKFFMKEGDRLPEEWLDSVYTHAQWLEQNLEYHLLANHYLKNGVALFFAGVYFEGTDA
                     ARWLAKGRQILADELDEQFLSDGGHYERSPMYHAISLVDYLDVLNLMTGSQRALHFDE
                     QQRFTTRVHEALRFLDDICLPDGDIPLFNDAALGIAPPPGDILTYGNRIMTYKRRSFS
                     AGLTTSEKSQTGYYVIRDASDMMVIDCGPVGPDYQPGHAHADTLSFELAVNGRRIIVD
                     SGVYDYEPGRRRTYARSTRAHNTLCIDQQDQSETWGVFRVARRAYPIYGRITGDGEVR
                     ATFEGAHDGYARLSGRLTHSRVIEYVSPGEWTVRDTVNGEGIHTIESLLHVHPDYRLL
                     LVGRSAEIRDERGNAILVIAPTPNVALTIEPGWYFPEFGLEYPNSTLVMTYRGTVPVT
                     IIYGMTKMDSTPL"
     gene            complement(12904..15057)
                     /locus_tag="PK224_12165"
     CDS             complement(12904..15057)
                     /locus_tag="PK224_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16538.1"
                     /translation="MRIHHAIPAWFVLATIVVAQSSFAGTTTVQVREMSSQPNYATTT
                     DRGDSVQLTDGVVTNFPMWTKREAVGWTATSPVTFSLNLHRGQARSRESHSGSLRIHT
                     AKGLYADVDVPHQIDVYSTDTAGRYLKVGSLSPDTQQFADKAHHWLTVPVSYATDTLL
                     IVLHARGEFLFLFLDEVVWQENEASGSLPKDRPVGSKTQALTDSVEGLRTNLLSRAVT
                     QSAVSPPPSSETELLWWAQDPWGHLPLDIPAKERDTLPKRFMVEGFQGEQESICLGLR
                     TWAGGSPSPVVLNVTGLPAESVRLYNVIPIVAANGQVVYDPLEPLGPEHTLALRTDKS
                     NYAWISFDLRTISVGTHTFTISILGSSGREVATATGELLVRAALPQVRKLAAINWAYI
                     NDNPIWRNPEVAFQDLLAHGINVFVLHPRNIPGVGLDGMWSAEHATAFNKFLTLARGK
                     GTLLLYLGWDMAKNPLGLSTQHPNLTYDAKRRLIDWVEKMSAYLEQKGFSKSEWAVYP
                     VDEPRGANITFLETVAHAIKSVDPAIRLYADPTSGRSGRTTIDDLQRLNNLVDIWQPN
                     LIFLREHAHTFFPQLNKPWWLYGNPRSPAKLGSPLHQYRFLAWWGWMFGASGVGFWSY
                     SDTNGSSAWNDLDGKYPDWAVVYEADNRIVSSRRWEAFREGLEDYSLLSAVDRGSAEV
                     ILGDPIRQNPSAVELEKWDSQKVYDLRHELMDRLR"
     gene            complement(15103..16980)
                     /gene="asnB"
                     /locus_tag="PK224_12170"
     CDS             complement(15103..16980)
                     /gene="asnB"
                     /locus_tag="PK224_12170"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB16539.1"
                     /translation="MCGIAGIVGYAEHCLQAMLAKIHHRGPDFTGSTALAAMHCFLGH
                     VRLSILDLDPRSHQPFVSDCQRYVLVFNGEIYNFSELRAELEQDGHTFRTTSDTEVLL
                     KWLIREGVAGLTRLEGMFAFCFADKQANTLLLARDPIGEKPLYYSFTVTNGTRRFAFA
                     SEIKSLLTLQDLNTSLDREGLFDYLRFLYTAPPHTLYQGIEELPPGHYLTVDLCDRKR
                     QPAQRYYDLESSLDKAPGMTFAEAARRFHTLFSRSVRLRLMSDVPVGVFLSAGIDSNA
                     ILAVAQLASTGSPLATFTLEYPRAQDESRAAKRIAANLGLVNTTIPFTQLEFFPALDR
                     VIHLFDQPFGNSTSIVSDMIAAKAAESCKVCLVGDGGDELLVGYPRYHALEHFAALAS
                     WPPMFHAGLRKLLNVFPETGRIAVPARRAKQFAKSLGKPMEEAFLDWSSYLDTPTLSW
                     ATGTREVQTGFYQDLVATFRRHQADPIRAAVIVDMKSFVPFNLLQNADRTSMAHSLEL
                     RTPFLATPLIHGILGLPTTAKVQSRKKALVTVPFERHLPAEILNAPKRPFNPPINVML
                     KANLPALKDFILGPTARIGTLLDRSFLATEMHDFSRGTRDNSTLLWGLATLERWLQRT
                     V"
     gene            complement(17067..18110)
                     /locus_tag="PK224_12175"
     CDS             complement(17067..18110)
                     /locus_tag="PK224_12175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB16540.1"
                     /translation="MNMVLIGSLPPPVGGTTVLFKQLVDELAACPDITLQVIDTSRTR
                     STPVSNLFTACKSLLSMLRHIPFTDVVSFHTSVGGAWQFGLVVHLLCRAFRRKWIFRG
                     FGGDYDVWHQQASPISRRIFEATVLRADTLLFERQASVHYFRQRTEQPVEWYANSRKC
                     TSTIPAPSSRARDRAERFVYVGHVKHEKGIGEIIEAARLLDEGLTIDIYGPLSADIGP
                     ETLTGKNLCYKGVLAPEAVVPTLGQYDVLLLPTYWKGEGYPGVILEAYCAGLPVITTR
                     WGGIPEIVSERSGILIEPRNSLALADAMRTLTHSSVRMSALRNGAALMAQQFSSRIWT
                     QTFLDLSRRLLNR"
     gene            complement(18367..19566)
                     /locus_tag="PK224_12180"
     CDS             complement(18367..19566)
                     /locus_tag="PK224_12180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16541.1"
                     /translation="MTTPDTRVLFFVPEWPALESGVLASQVFAVAGFLARKGFDCQFV
                     GAERSEEQAHRAEAFIEQRYSIQAKVFDLFPSRPNALSLAATTYNTFARLRSFARTFA
                     PTHVYSRSCIASIFGRRLASDSGAVSIYDLRALLASEVALSRQRGTIAVRLINFIEER
                     EMKRAERLATVSKRLNLQIQRQVSRTCTAVIPSCYDPQRFYLEAGAREQIRDEFRVTA
                     DEKLLCYSGGTAPWQRVSDILTLFDELSRRRDDVRFMILTPDAGHLRSTIASLTYPSR
                     FHLKNCSHDDVRRHLSACDAGVIMRHDTMVNNVASPIKVAEYLACGLPVIMTQGIGDY
                     SEDLVRERVGLLLDERKDLPEQVIQFIFNPDFDGIRDRASRYALGHLTLDANWKQYQD
                     LYRPRGA"
     gene            complement(19609..20703)
                     /locus_tag="PK224_12185"
     CDS             complement(19609..20703)
                     /locus_tag="PK224_12185"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRB16542.1"
                     /translation="MIKPGPVLLAALVFVLYGAVQTILSDNMTLALPYYKKYLEGIGL
                     LTILFLTVQSRNDLGRVLIWWAVVAGLTTIVKAAHIYFGDNTVLYRTMEAMFLNDEFG
                     LEHRIHIRHGGETGRRFLLPGEEPNYTSASLVFPLALALAFYSAGKAIAKLFWAGIAC
                     MTAIACVGTYSRSGFLATALVGGLYVLRGNAVKAIIPVGSMAAAFAVAVVSIPSLHKR
                     IFGIGGAVNEGATGRFDLWRQAIDMWFDSPIFGNGMSAFYDRYHGAAHNTYLQVLAET
                     GVVGFALYGTVIAVAIRVGRRLQKSEAFSKAVADIQLSKSALSGLFAFCFMIATVTYQ
                     DVKLFWLALGVYAAIYLIQRSPRPLNSREC"
     gene            complement(21072..21839)
                     /locus_tag="PK224_12190"
     CDS             complement(21072..21839)
                     /locus_tag="PK224_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB16543.1"
                     /translation="MERPTTHRAFVQQLLEREDGRFNTVDYVGISLPRFTYTITLCKN
                     LKPDQDTKVLDVGRSYLSFLLAQHYVSVCTLGIPLGEHGYAHEYAGLDLERRPQNHIT
                     FNLNDAQEQGIETEQQFDLIVFAEVLEHLYTAPELVLYALRNLLTENGLLVCLTPNAA
                     ALPKRLRLLQGINPYERIRINRNNPGHYRECTKGELEQIGRSAGLEMIHHSFCNHSPA
                     RTHLLPTNKSQVFELLCRINPAFCLGQTAVYRKGPTI"
     gene            complement(22555..23583)
                     /locus_tag="PK224_12195"
     CDS             complement(22555..23583)
                     /locus_tag="PK224_12195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB16544.1"
                     /translation="MRVLFLTYPRIGLNRGGLQIQIEETAKGLTELGVEVVFYDPWRD
                     QIPEVDLCHVFGIDGAVISHVQRSIGLGKPVIVSSVLNLFQTPPFLTRLKTLSSGIVP
                     GMYSDLRRAKMMLHAARQIIALNEPERAQLSKIFSLDSDNIVTIPNGLNKQFGSIDPS
                     LFKQRYGIKGFVLNVASVEERKNQLTLIRAMKGTPYSLVIIGKVPPGQENYMERCRQE
                     ADENVIFAGGFAHNDPILASAYRAAKLFVLPSTSEVMPLTLYEAAVAGCRILVSQNVP
                     IANSINHLIPTFPPGSPTQLRALIAREMERPSTSDLQDAVSDMPTWRDVCEQIVTIYT
                     RARTPQPA"
     gene            complement(23616..25076)
                     /locus_tag="PK224_12200"
     CDS             complement(23616..25076)
                     /locus_tag="PK224_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRB16545.1"
                     /translation="MEKIKAQYLLGVVLSGGANLLGMGASLLTIMVAARLLSQEELGS
                     FFLLLLVAQVTALFGDIGFQNTAIKTLSQLQADSPEFIHTARYLLTIAIATSLLACLC
                     MGLTVPFLTSLWPSPNFVSHVGYIAPVALLTTGMQTVMSLLVGAKQFRVLSSLSVGIE
                     VLRALFSMGGLFAGLGISSLLWGMIGSRIIGIAVIWYMLPLLFSIQLRHPRSADFLKF
                     GGWLYGCSLVSVIMVRTSDTILTTYMGTAALAVYSAAMQVPSVLQRAFESIRPTLLGY
                     ITAQNSTYKNPQITLIRMMTALLAVAATGLIALAQPLMTLLYSEKYEAGIVIMQALSV
                     WVVFTIINYLYSIILIGNGQSKKAFVLTLPQLCTIVIASGVLVPRYEGFGAATALVLT
                     AFLGNLIGARLVAGDNVSTRHLLTTGFLRAAVPLLLFLLAAASLASSFALLCGFAGIT
                     LLLLMAFKAVTLEDCKVLRATLLGMGERMSALPSRA"
     gene            complement(25161..>25341)
                     /locus_tag="PK224_12205"
     CDS             complement(25161..>25341)
                     /locus_tag="PK224_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRB16546.1"
                     /translation="ERPETVTIRTNELIGTDPCKLPPALARVMTGQWKTGAIPPKWGG
                     KTAERIVGHLEHLLS"
     assembly_gap    25342..25441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25749..25919)
                     /locus_tag="PK224_12210"
     CDS             complement(25749..25919)
                     /locus_tag="PK224_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16547.1"
                     /translation="MTIGTDELIGTDPRKLPPALARVMAGQWKTGAIPPKWGGKTAER
                     IVGHLGTLLASG"
     gene            complement(26010..26375)
                     /locus_tag="PK224_12215"
     CDS             complement(26010..26375)
                     /locus_tag="PK224_12215"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRB16548.1"
                     /translation="MKFLLDMNLTPDWLRVFTAAGWDAIHWSTIGDHRATDEVIMKWA
                     RDNAYVIVTHDLDFSALLAATRAVGPSVMQVRTQNVLPDALRVVLINAIEQFRRELEE
                     GALISIDPQRARARILPFD"
     gene            complement(26375..26602)
                     /locus_tag="PK224_12220"
     CDS             complement(26375..26602)
                     /locus_tag="PK224_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRB16549.1"
                     /translation="MKELNRITMDPAVMGGKPCIRGMRVTVGTIVGVMATGKAIEEVL
                     AAYPYIEREDILQALTYAAWRSEEKDLPLVS"
     gene            complement(26672..27772)
                     /gene="wecB"
                     /locus_tag="PK224_12225"
     CDS             complement(26672..27772)
                     /gene="wecB"
                     /locus_tag="PK224_12225"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRB16550.1"
                     /translation="MKRIDLIAGARPNFMKIAPIIDALNAARSNGSELRFRLIHTGQH
                     YDRAMSGSFFEELGIPDPDINLEVGSGTQAEQTAGIMVNYERVLTKEKSDLCLVVGDV
                     TSTMACSIVARKMGIPVAHVEGGIRSNDWTMPEEINRVVTDSITNWFFTTSDTANENL
                     RRAGITDDRIFFVGNTMIDTLLKQLPRLRPPACWSSLALEPNRYFVVTLHRPANVDGE
                     QQLLGLLKAIADGTHGLPVVFPVHPRTAKNLREAGKTLPSMHYVDPLGYLEFNYLVKH
                     ARGVITDSGGITEETTVLGVPCLTLRDNTERPETVTIGTNELIGTDPRKLPPALARVM
                     AGQWKKGAIPPKWDGKAAERIVAHLKDLLAGQ"
     gene            complement(28152..28391)
                     /locus_tag="PK224_12230"
     CDS             complement(28152..28391)
                     /locus_tag="PK224_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16551.1"
                     /translation="MTRCTRLTLSLVFLSLLAGCSTSEWVHPNRPKDQFTLDYNKCQA
                     DTLRDPKLQQGIQLLIIQATERCVQKQGWRLVEKE"
     gene            complement(28496..29395)
                     /locus_tag="PK224_12235"
     CDS             complement(28496..29395)
                     /locus_tag="PK224_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB16552.1"
                     /translation="MTRPAASRTLLVVEPCAETLAMVLEQAKTRDLSIMTAPDPHVAL
                     AMMDMASPDVLMTDLFLPEPNGMMLIREALKRNARTAVIATTRAGNEQALLEAVRAGA
                     GDCLYKPARAEELGMALDRALRRIPQTIDDVPGIEQLEYRLVIGTDPGDVESCVTWLI
                     ESTAMGLPETQRLHLRTTLIELIVNAVEHGSLEIFYREKHEALSTDRFDDLIAERRRN
                     PRFAQRRVIVRALYDKFHRRMRYAITDEGNGFTWNRFLTRSDQPCDSRDANGRGVFLA
                     KAFFPDLTYNERGTEVTFSVPLP"
     gene            complement(29392..29751)
                     /locus_tag="PK224_12240"
     CDS             complement(29392..29751)
                     /locus_tag="PK224_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRB16553.1"
                     /translation="MEDQPVMDLAAAMERFDGDQDLFLTLAEMFLERSPETLAVIQTA
                     LADKNLPVLAKEAHKLKGSAMEFCARPAVAAAAQLEESARQSAVSEMAAIGEQVHAEV
                     QRLTAALRMIVEKGFPA"
     gene            complement(29751..31016)
                     /locus_tag="PK224_12245"
     CDS             complement(29751..31016)
                     /locus_tag="PK224_12245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRB16554.1"
                     /translation="MAEPAHRIVDDPLAGKAAKPMPVILLVDDDQITRISMAGRLKRL
                     GYRVIEAGDGVSGLAAIRAQRPDLVILDWMMPGLDGPSVCEAIRADGELRSSQVVLMT
                     AHDQPEQIAEGLSRGADDFLSKAASKQEVLARVQASLRSNALVREIERTRDDLDRSHR
                     LLSAKQAELESELQSAADFVRAQLPLPGRPAPGISMQWAYQPSLALGGDLFQVSAWGS
                     ETLGLYILDASGHGVSAALRAVALMSFLQEDNLTKAVGSFDPGAIITEANRRFPLTQD
                     GEYFTLWVGRLHLPSQTLSYATAGHCGAFRHSLQGASQWLSSASLPLGFDPTISFDSA
                     TAQLLPRDRLYLLSDGIYEAPSPTGELWGRDRLQATLERHRTHTLAHSIGDTMTTARQ
                     WLGGDIFPDDVALLGIEVLDSSGSAKEKS"
     gene            complement(31040..31390)
                     /locus_tag="PK224_12250"
     CDS             complement(31040..31390)
                     /locus_tag="PK224_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRB16555.1"
                     /translation="MQITERRIGQSIILDLTGELSYANRTTFKAAVERSKQGGCRHLI
                     LNMQGVRFLDSSALGTLALLAKSFLATQGMVSLLNPQSYVKEIVTLANLHQILPVYTS
                     EQDALAGSRLPAAG"
     gene            complement(31423..31761)
                     /locus_tag="PK224_12255"
     CDS             complement(31423..31761)
                     /locus_tag="PK224_12255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRB16556.1"
                     /translation="MKITQEIHNKKATLRLEGNFTYTQRKAFQEALKVVCSDPIEEIV
                     IDLSQVAFLDSAALGLLMISHRQLGAEKRKLSLAYPQPTVRQIIELANLHKTIPMIDA
                     GVSTLAKKSA"
     gene            complement(32081..32542)
                     /locus_tag="PK224_12260"
     CDS             complement(32081..32542)
                     /locus_tag="PK224_12260"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRB16557.1"
                     /translation="MDLKTILWYLVLFSVTAAYLIVLAGVRSAKTHDVSHHSHRMIVG
                     CTITGIWLVAYVLNQLLFGRESFRGSDSQYWSLYVPILAVHMLLAVTTVGLGAYNLYM
                     GLHRLRYGSVGAMVTGMTTHRRMGTALVWTFSGTMVTAYLVYLMLFVWFKR"
     gene            complement(32796..33575)
                     /locus_tag="PK224_12265"
     CDS             complement(32796..33575)
                     /locus_tag="PK224_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRB16558.1"
                     /translation="MAKKKHEEHENHERWLVSYADFITLLFAFFVVMYSVSSVNEGKY
                     RTVSDSIKAALNPVNSIPSSRLPFSIGDTKPKMIPPESDSINDPVIRRMREIVTRINP
                     GLKGDIAEIKIIETGHGAIMISLPESILFQSGEARVRSEALPLLKALANVLIEMGRHI
                     RILGHTDNVPIRTAQFPSNWELSAVRAVMVVRIFSELYEVPITHLSATGFADSKPVAT
                     NDTPEGRTKNRRVEIIVLENHASEETLDALLPKEGASAPLP"
     gene            complement(33653..34423)
                     /locus_tag="PK224_12270"
     CDS             complement(33653..34423)
                     /locus_tag="PK224_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HRB16559.1"
                     /translation="MDIATILGIVVALGSIIGGQALEGGHLSSILQLTAFIIVAGGTF
                     GACCVQNPLSVVIKALTSATLVISNPPHDVKGTITQILDLANVSRKQGLLALEGKLKD
                     LHDPFFKKGIQLIVDGTDPKLLQEILEIEVEHHEEEGVHAAKVWEAAGGYAPTVGILG
                     AVLGLIHVMENLADPSKLGGGIAVAFVATVYGVGLANLFFLPIANKMKFKLKEEAGLR
                     TMVILGLVGLAQGENPRLLQEKLESFLPPHERSKEDKK"
     gene            complement(34636..34866)
                     /locus_tag="PK224_12275"
     CDS             complement(34636..34866)
                     /locus_tag="PK224_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB16560.1"
                     /translation="MNDAITATTKVTLDGASPSDSEILTVLDVARFLRVPKSTVYKLA
                     RLGQLPASKIGKHWRFLRRDIHDWMHHRTKQD"
     gene            35065..35994
                     /locus_tag="PK224_12280"
     CDS             35065..35994
                     /locus_tag="PK224_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRB16561.1"
                     /translation="MAMIVSIGSGKGGVGKSVIAANLSMLLAKQGKRVVLADLDVGGA
                     DAHILFGMLNPPRTLTDFVERRVERLDDVLQQVSAHPFLQLIPGTGDTLATANLPYAK
                     KKRLIRHFRQLQADVIIVDIGAGTSYHALDFFLMADHYLTVATPDPMSVLDLYRFIKL
                     AAIRRVLSAFLSRDAVTETLSDRDFCSIDEVIQAVSETDPNAKETASRTLQGFHPYLI
                     VNRVSGKSRVNVLQLKKLLQEYVGGDLMMLGEIPDDPAMTRAVRSYLPVVEFEPTAPA
                     SLALEKAAQTLLTLLAHPETTQAESAPSPEQAA"
     gene            36162..36293
                     /locus_tag="PK224_12285"
     CDS             36162..36293
                     /locus_tag="PK224_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16562.1"
                     /translation="MMTKERHVCARRRAGEAAGRLNENSLAGRQDRTPEQVFMNSPG"
     gene            complement(36290..36901)
                     /locus_tag="PK224_12290"
     CDS             complement(36290..36901)
                     /locus_tag="PK224_12290"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB16563.1"
                     /translation="MTPSHDRFRQAPHTDSKNEERREWLRIEDRLLLEYRRFDEPAEA
                     MNAHLPPATEDTIATAVSKPTIDLLVRAGETFAGSPLLPWVSKIDWMLETILKSLVKS
                     HPGSVAIARLTDVDISAGGLGFDTPRQFQAEDLLVLKVILPPFSMIEATARIIRVTPA
                     ERDSTGYHVATQFMELGGDEQELIIRHILQVQAERLRARKSAH"
     gene            complement(36959..37303)
                     /locus_tag="PK224_12295"
     CDS             complement(36959..37303)
                     /locus_tag="PK224_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16564.1"
                     /translation="MSAASSLDTGPLHDCRSIELLTIQALEAARAGDWDQVDACYSVR
                     GIRLAACELDRTVAQQLLRMDEEVRAAILVAQAGISGQLADAAQVKRHLRQLRESSGQ
                     LASERVTIDREA"
     gene            complement(37290..37682)
                     /gene="fliS"
                     /locus_tag="PK224_12300"
     CDS             complement(37290..37682)
                     /gene="fliS"
                     /locus_tag="PK224_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HRB16565.1"
                     /translation="MIATAANAYQQTQVMTASRVQLIVLLYDSAIQSMELAREAILTN
                     NYKDKARFLDRSIAIVGELSSVLDFERGGEIAVSLHRLYDYMVQQCIQANLRHNGKHL
                     DGPIRCLTTLREGWHVVARQEAVAHVGS"
     gene            complement(37697..39088)
                     /gene="fliD"
                     /locus_tag="PK224_12305"
     CDS             complement(37697..39088)
                     /gene="fliD"
                     /locus_tag="PK224_12305"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HRB16566.1"
                     /translation="MAISFGGLGNGVDFGQVVSELAKVQRLPIDALTAKKKDLQTKLT
                     DYGALGNKLLALQSAANALRLPSSFNRSTSTVSDDNILTAQAGAGAATGSYTVQVTQL
                     AKANQITNKAVKAVSGTTEVVSSGAGTFTFRVGSGTDQTVTLSAGATLDELRSAINDL
                     GAGVTASVINTGTEASPAYRLTLTATASGAANTITVVTDTTTLDFTNTSGTGGNDTLQ
                     AGQNAIVVIGDPDQTTISIERASNVISDAIPDVTLTLKSKTVTTPDPEPVTVNISNDP
                     ASVKTNIKTLVTAYNDIVKFVNDRTGYDITTKTGGIFFNESTAKNVLSQLRTAISGEV
                     SELSTYKSLGAVGFKTERDGTLTIDDAKLDSAMASNYVATRALFVTQTTSSGIADRIV
                     KAVDFLDSVDSGAFTVRKNSITSQISRLTDQIGRKEDIASQYEERLRLQFASLDGLLQ
                     KLQSQTSALQALR"
     gene            complement(39116..39475)
                     /locus_tag="PK224_12310"
     CDS             complement(39116..39475)
                     /locus_tag="PK224_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HRB16567.1"
                     /translation="MVHQVSNHKDLLAAATHASQTVPQYPPVRSPEKQVSMPEHSGGE
                     VNKKELDQALTRVREVFQMADSRVEFSIDPDLDRVVVKILDGESGTIIRQIPQQEVID
                     LAKRLETPTGLLLHHKV"
     gene            complement(39542..40369)
                     /locus_tag="PK224_12315"
     CDS             complement(39542..40369)
                     /locus_tag="PK224_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRB16568.1"
                     /translation="MALVVNTNVASLAAQRNLGINQAQLGRSVERLSSGLRITRASDD
                     AAGLGVSETLRAQIRSINQANRNASDGISLTQIADGAASSIGSLLARMRELATQSASG
                     TLGSTERSYLDQEFVALRSEIDRISVTTEFNGQALLSGSSNTFDVFIGFKSGSGNSLS
                     VALTDLDVASVGLTGASVSTAAAAQAVLATIDGAISSVATARSNYGSIQSRFEVAIQN
                     LTVTAENFTAAESRIRDADIAHETSVFTKNQILTQSGIAILAQANSLPQQALTLLRG"
     gene            complement(40608..41150)
                     /locus_tag="PK224_12320"
     CDS             complement(40608..41150)
                     /locus_tag="PK224_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16569.1"
                     /translation="MHITLDGEQWQLPDDTSLMNALAFLSDKAHAQHRIVTSLTVGGR
                     AINDRDLDPAFLNQCGPEVGEVAGRSQSLHAIITDAKQAIDRFAAQLRADGLVLLGPL
                     RSGTGQVGTIDAWLGRLADYTEMLEAGQAQGVAGLSSAPLLPWIQELLGARTFADPIR
                     VADLLEYELLPLLTESSQAA"
     gene            complement(41196..42872)
                     /locus_tag="PK224_12325"
     CDS             complement(41196..42872)
                     /locus_tag="PK224_12325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRB16570.1"
                     /translation="MSKQVLLINITRMGDLVQMGTLLQRLQYEWPGAAVDLVVDRRFA
                     PIAKLLPHLRHIIEYDFHRLVDESRAQTKDVVTLYREMTGWAAPLIEARYDRVVNLTF
                     NRRSGLLASYVGAKEIRGIAAPKDGDVAIHNPWMAYLTDVHSQRQFNHFNLVDIYALG
                     GSGTGPFAPLSLDIPPGTAQWARDFFAPYDGQRMPWIAVQVGASDPMKAWRPELFGQT
                     LAALSRQTPVGYVFIGTESERKAIDTAQMAYRQAGGRGPLCDAVGRTTLPQLAAVLAQ
                     CRLLLTNDTGPMHLAVGVGTPVIDLSVGHVDFRETGPYGPGHWIVQPDMGCAPCGFDQ
                     VCLHHACKDRLVPDQIAALCLHVLTGAAFPETVTGIKIYRSRVDEDGLGSADICAGRE
                     DPTVNWYGRFWRRFWFEQFTGRPSLVPMVLEPPPDWKESMALLDRLLPLAAGLVSRAK
                     ELVRLTARRPLPVRALQHMQLEENRERQTIVALSLQSPATASLAVALVRDTHNDDGHE
                     LTGMAEARLATYRRWQNRLHTVAAGFRAFKTPQGFAPRGTRTAIPMVPIG"
     assembly_gap    42951..42986
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<42987..>44391)
                     /locus_tag="PK224_12330"
     CDS             complement(<42987..>44391)
                     /locus_tag="PK224_12330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16571.1"
                     /translation="AVLISDLNEFHDALVARPLGSWTDHILWLSGTPVEIADRLSKTG
                     RALRCLSYAPATHTDSDFHSAFEEALRRAVARQAGGQLTIALVGPIYGGSLPIARYVR
                     RALETLGHKVHWIDHSVHASSYEAMGTLKDARNRQLMQGRMAEVLSQWTLASLAESPP
                     DLVLSLAQAPLTLPVLEHLRKKKLLTAMWFVENYRHLTYWQQMAPGYDYWFVFQRGAC
                     LDAFRQAGARQVSFLPMAADPELHRPMDLSDEERRTYGADVSFVGAGYPNRRRLFPAL
                     LRRPWSFKLWGNEWDGADELQSVLQLNGARIDTDTCMKVFNATAINLNVHSTTGAGLD
                     PQADFVNPRTFELAACGAFQFVDSRSQLPEFFTDRELVSFRNFDDIPGLVGQWLGDPV
                     ARQAMAAAARTRVLGAHTYVHRMRDLLGHIGLSQPDRVGAVLRGDRQQEALLSRCAGD
                     APLESLLKAFPAGQRVE"
BASE COUNT         9697 a        12274 c        12678 g         9606 t
ORIGIN      
        1 gtacttcgat gcgtcacgtc gcattactcc cgaatcaatt gttacagtcc actagctgtt
       61 cgatctcaag gtaccatcac gacgtcgccg cgctggagca gtatgttttg cttcaggtcc
      121 ctccccttct ggatgtcacc gtagcggaag taaaagacac gttggtctcc gtcgtcaccg
      181 ctctggcgcc ggataatttt aatatcgttc tcggcggcga agggcgtgag tcctcctgca
      241 aggctcagtg cctggagtac atccgtgtag tggccgatga ggtattcacc aggtttgttc
      301 actcgcccca tgacgtaaat cttgtaactc tgaagcttga taatcgcgac cgatacatgg
      361 ggattcggca cataccggga aaggcgtttg gcaagatcct gccttatatc ttccacggta
      421 tgtcctgctg caggaagatc gccgaccagg ggaaatgaga tcattccatc cgggcgaacc
      481 acggcttctt tggtgaggtg ttcatccttc cagacggaga tcatgagcac atcctccggg
      541 ccgagctggt aacccggatc tgccaggcta gattgagccg acgtaggttc gggcgtgacg
      601 catcctgcca agagcatgat cgtgtacacc atcaacgcga gctgatggat ctgttgaaat
      661 cttttacctg aacgggacgg actgacaatt ttcaccctct ctcctcctat cctgtgactt
      721 ccgcaagaac ggctttggct tcatccatgc cggggaacgg ctgtttcgag tttaatgcct
      781 tctttaagtg agcttgagct tcgagtcgat ggcccgcttt aaaatgagcc atgcccaggt
      841 gatagttcag cagggggtgt tgtggggcct gggcgacagc acgctgaatg acacggaggg
      901 catcatcctg atgtcccatt tttaggtgta cccaccccaa tgtgtcgagg aaaaatggat
      961 ttggcgcacg ttgctcaaaa tcacggctca aggccaaggc gcggtcgaga ctcttggtat
     1021 cccccttctg atccgtcaaa atcgcggcca aattgttggc cgccatcaga tcgcgaggat
     1081 tttgactgag aatagtttcg tattccgtga tggccagatc aaactgtccc ctatccccca
     1141 ggtgagacgc gtagagcaag cgcaactctt cattcttcgg attgacctgc aatccgtgtt
     1201 gaagaatttc gttggcctcc tcgccttttc gctgcgaaag tttgagtgtc acccagttga
     1261 cccagggagt gctccattcc ggcttcaagc cggtggcgag tcgaaattct gcctctgcgc
     1321 cggcagggtc tcccttgaga ataagaatct caccaaggag tccgtgtgca aattggtgac
     1381 gcgggttctt ggcaatcaaa ttggccagac gattctgggc ctgagccagt tttccctgac
     1441 gggtatcgat acggaccaat gcaaacgtcg gctccggcgc atcgggattc tgggcagcgg
     1501 ctcgttcaaa tgctgctata gccttgtccc attgcaggcg ggcctgatag agattgcctt
     1561 cggctaaatc cgagacgaac cggttggcac cggctgctcg aatgcgggac agcgtctgat
     1621 ccgacccgtc ccaatcacgg tccgccgcct gcaggtttaa gagcatgccc aaggtgccga
     1681 gattagtcgg cgcctgcttc aacaattcct ctaagcgcaa ccgggcatct ctggtccgac
     1741 ccgaggaggc atcaaggatg gcaagagcca gatgggcttg gtacaaagtt gggcttaatg
     1801 ctacggcttt ttccaggttc tcacgagcca ggttcgtttc ctccagcatc aggtgcgcct
     1861 gccccaagag ggcgtaggct tcagacttct cgggttgatc cttgagcacc gttctaaacg
     1921 cttgaactgc ctctcgcccg gctccatgtt gaagggcgat tcgtccctgt agcatgagtg
     1981 cctccgaggc gcgcgggtta ttgcggagga cttcctgtaa ctgttcggtt gccgcatcct
     2041 gcttgccctc cacccaatca agtgtcgcaa gcttgacctt agccgtatct ccagccggtt
     2101 tggactgatt tcggtcacgg aggtcctggt acgtcgcgcg ggccttgtca ggctgttgat
     2161 gcatttcata gaggcgtccc agcgccaatt gaatgttgac ggcgtgagga agtgcgtgac
     2221 gagcctctgc gagcgccgct tcggaggctg ccacgtttcg tcgaccggct agataatcag
     2281 ccagagcgag gtgtctctgt tcgctatcag gctccagtcg aagggcttcg cgcagggtcg
     2341 cttcagcctt gtcgtactgc cgttgttgat caaagaagag agcgagcttg agatggtggt
     2401 cgtagacttg tggttcgatg gtaagaatgt tttggaacac cgcttctgct tttgtctgat
     2461 tgctcacacg catgtaagcc atcgccagtg aattcagcaa atcgaggtct cgtggatggg
     2521 cctccacggc tttctttagg actgcttcga cacggtgggg tgcattttga agggaataca
     2581 gggtgccgag aagaatggcc gcatcgggtt cagtgggaaa ctgtttgacc aatgcctccc
     2641 ccacggctgt ggcttccgtc agtttgccct cagccgccag aagcgcgcca tgtattgctc
     2701 gggcggacac atgcccggga tacagccgga ggagttgatc tgcggtttca cgggctttct
     2761 cgtccaggcg cccttgcaaa tagaatttgc ctagttttac cagtgcttgt tcgtgtgtgg
     2821 ggttgagttc aaccacttgg aggtaatgcg caaacgcatg gcgccaatcc ttttcctttt
     2881 cctcgacctg ggcgtagaga aagtaggctt ccgagtcttt cggatcgatc ttgagcacat
     2941 tccgcagagc gacacgcgcc ttggggaagt ttcccgcttg catgtattcc tgtgcctttg
     3001 tccggtactt tgccttacgt tgctccgggc ctccacaccc cacggcaaga atcaacagag
     3061 ttgccactcc caactttcca agcacggtac atcgctggaa tatcgcaccc atagtggtcc
     3121 ttttccttgt tacagccacg ctcggatggc aattccgaag aggagccata acgcaaccag
     3181 tcctagctgc gtcacttgct gagcgaacac gtggagcagt agttcaaacg aaaaaaacaa
     3241 cacgatcaat ttagccgtca ggaagctgaa atgaatatcg ccgatttgca tttctggcaa
     3301 cgcaggcacc atgacggcca gcaggaccat caaatagtcc agtggagtag tttggaagcg
     3361 atgttcggaa ttgaatcgga tcgtcagcgt caccagcaag gcaagtccga aaaaaaacac
     3421 gtgaatgggg ctgaggatat ccgttgaggc agccggttgc tcgctgagat acatgatgaa
     3481 ggtgctgcca acgtacaatc cgccacggac aaccaacgtc gaggttctag ggctcagcca
     3541 aagtccgaat agcaggactg caaacaagat agaggccaca tacgccatat cgtctgggac
     3601 tcgtttgggc aaaaaaacgc ttgcaatgag gaatgcgggg acagcgatcc ccaagagccg
     3661 tataggctcg gtggtcagcc agggggatga ggtgatcgtg ctcgttcgcg taagaaaacg
     3721 atcggcccgt ttcaacagcc gattcgattg aatgttccag gcacgggatc ggagaaaggg
     3781 aaacagaatc agggccgata cgaagcagta ggttccgagg atggccaact cggtctgcca
     3841 acgcaacaga taggccagcg ttaccagcat cgcctgcaga aaataaatca tcagcacggc
     3901 ttcatagtgc gaaagcccca tcgccaataa tttatggtgc aagtggttct tgtctgcgac
     3961 aaagggtgag cggccttcct tcagccgttg gagcatgacc ccgatcgtat cgagaagcgg
     4021 caggccgagt aataagagac cgaggggtgg agtatagggc cctcgttgag gatcagtcag
     4081 gacgatcgcg gagacggcca tgaaaaaccc caaaaattga ctccccgcat cacccatgaa
     4141 gattcgtgcc ggataggtat tgaaccgcag gaaacctaag agccccccga gtaccggaac
     4201 catgagcagc gtgacggtat ggtcgccgct gagataggcc aggcaggcca tgccggcgaa
     4261 gctgagcagg gacaaacctc ccgccaaccc atccaggcca tcggcgaggt tgaccgcatt
     4321 ggtaacgccg acaatgataa ggatcgtcag cggataggcg atccagagcg ggagttcgct
     4381 atcgacaaac ggcaaagcgt tcagatgaat atcgccctgc catatgacga taagggcgcc
     4441 gaggatttgc cccaggaact tgatcgaata tggcaagccg acgcgatcat cccacatgcc
     4501 gaacaggaga atcgtgacgc cgcccaggag gcctgctacc acaatgtcat ctttcggcgc
     4561 ccagatgagc atggcggcaa atgtgccgat accaaaagcg atccctccca ccttggccac
     4621 ggggttggag tggacgcgtc tttggtcttt gggaatatcc aggaaatgaa gacgactggc
     4681 ggtggccatc agcggcggaa taagcgccat acagatgatt aaagaaccga cgaaactgaa
     4741 aaatagggtc gaaaccatgt gttccgttct cgagtagtgg gtcagtctgg aatgtagggc
     4801 tgtaatcgtg gaccgaccgc ttcagcaagc gcgagtaccc gctgtcggga aacctcctcc
     4861 gtctcgctgg gtcgtatggc cgatgcaaga cgtatgagcc cgccatcggt gcgttgccgg
     4921 acgatggcat cccacaccaa atacagtttc acgagatatt catttgtcag ttgacgatca
     4981 cgctgtttga accaatagag cacgacctga cgctggtcgt tttttcctat taggacgaga
     5041 ttggcggaca gcgggagaga cacgttcggc cctcccaacc ggacgggttc tacggacagg
     5101 atttcccatc ccccgcccgg gatacagctc tgtggcgaat gcacggactg accttttctt
     5161 tgcgaacgat aataggccat gtacagcgtc accggagacg actgaccgaa gcgataatca
     5221 gcgaggagat agtcgtcaaa tcgcagtgcg tcgatgtatt gagattcgag aggataggag
     5281 cttccggacc agccctccac tagcatggga aaatctagaa acggttggcg cgtaggaacc
     5341 atttcatgac ggtcgatgat ctggggcgat atcagtggga gcagtagtaa cactccaatg
     5401 ctacagacat agggagacag tgaggatgac gggagcagct tcgctgtgaa gccgtgagtt
     5461 ccggtcgtag agaggagtga aggcttgctg gcaacgccct cccctcgaga gtccgtccgg
     5521 cagcatgggc cgatcttgcg caggatccac atttcaacaa ggaggatgcc gaagctcacc
     5581 atgaagaagg cccagccttc gaatgcatgg aaaaacccct ccgcggccgc ttctccgaaa
     5641 tggtcgacaa gcacaccgat catggcgatg cgaaacccat tcagcagaat agaaatggga
     5701 atgctggagg cgaagagcac gatccgcttc cacaatcgat cctgcagcag gtacgcgcaa
     5761 aggagcgcga ggctggtgag tggaaacaag tagcggagtc cgctacaggc ttcgactacc
     5821 tgcaattgaa taggccccag gtcgataacg ttgccttccc ggaacgcagt cacgccgacc
     5881 aattgaaggc atcccacgcc gagggctgat gacatcagtt ggaggtgtcc cgagagtcct
     5941 tgatatagaa attgtggaag agggatggct gttaaaaggt atcctagcgg gaaggaaatg
     6001 gatgcgaccg cccgcattcc gagtgcggct accagcaatc cgatgaggat acaccagaga
     6061 gacaagtgga tgagaatgaa cagtgtggca agttcgccta ctatgtacag gaagagtcct
     6121 gaagcgatga tcaacagccc ccaccaggat cccccattcg cccgttctgc caattccgtt
     6181 ctctgttgcc agatcaagaa cgctgaaaca agtggcacga gcaacccatg actataggta
     6241 tcattgccga tccattcctt caccagcggg attaagcttt catagtagac aaacgcaaga
     6301 atccccagtg ggagcaggac agcggcaatc acatgtgagg aagtcctcga ccacagcagc
     6361 cacgatagtt gtggggggga aggcagaatg gtttgagtct tcctggtcat tgtcgggacg
     6421 attcttcagt cagcggcgtt ttgggctagg gtgcgggaat acaacgggca aagtgcggcg
     6481 catacgcatc gtcgcatcag gcgcgcaacg agcttccacg agaccatccc gacaaggaaa
     6541 atgcctgaag agaatccgga ggagcaccct ctcccatcca gatcctggtt atgaattcgt
     6601 gcgggagtgt cccacggtat tccgcacgca gagtcgtcgt cggcgttctt gatccataat
     6661 gcgatgcatg ccatccacgg attggattct cttctccaca gtggagtgac acttggccgc
     6721 cctccacgga gcactgaacc cgttcctcac cgaccacaaa cagatgggat gccggctgcg
     6781 gcacgggagt cacgccgaga tgccaattta actccagatg atgcgtgcct gttcccgtga
     6841 gccaatccca tatcaaccac gcgccctgag gctgataaac gatgcctcgc caatggctga
     6901 ctccgaagcg ctcaaggtaa ccgtaatggc gggcaagaat cgtgatcgtg ccgttgggtg
     6961 cctcttcccg gtagatcaag tgagtagcat agggctgcga ccataagaag ccggtctcct
     7021 gaatcgcctg gtcttgcccg ttgatggtca ccgtgttatg tgcgcgcgtt ccacggaagt
     7081 actgccgcca agtcgtgtcc tgcgtaacgc tataggtccc gggatcgatc aggacttccc
     7141 ggtcgccgat ctccaaggtg actgccaagg catctgcgtg tccctgagaa tagcggggag
     7201 ccatgcctaa ttcaccatga ttgaataaca gtcgctgtct attctgtcca tgaatcagtg
     7261 aataaccgga caaatgaaag gaagtgagcc ctggcgactg cgctttcggt gcggaaacga
     7321 aatggagatg aggcgagagc gcgtatccgt cgtcgccttc gccgatctgc gggagtgctc
     7381 catcgacagg gcgcatcatg gcttggagga atgaactgct ccgggcgaac gcgtcttcaa
     7441 gtttgttcgg aacgggccgg tcaaattgtt ggagcagatg gattgccaat ccatagagat
     7501 cagagacaaa gcgcagcgca caaaagcttt cttcgcggcc tcctccgtcc tggcggatct
     7561 gccgcgtcgc ttcgttttcc aataggtaga gcccaaaggc gaaccatcgc tcggccatgt
     7621 ccattttagg aaagagcagt cctgcataga ggaggcccac tgcctcggcg atcgtatggt
     7681 gtgcggtcgt actgtacccc gtggtgcgtt tccggataag ttccgcatgt tgacccacga
     7741 gattgagcac tcccacccat atctcctgcg ggttggtcag ccattccctg atcagatcca
     7801 ccgtatgaca cactgcgatg aggcggacgg cgcattccat gaccgagacg taatggatgc
     7861 ccaccagggg cggattggcg gcaatccatg agagcaattg agcttcgatt acctctacgg
     7921 ctttacgccg atgatccgga gctgcttgct gggcatagag agccagggcg acgaggtgct
     7981 gaagccggga cggctcccag gctatgcgga tgtctccata gggattgtcg ctgccgcagg
     8041 agatgcgatg aaagaatacc tgcggccatt gctgcttcgt atccggggct tcatgccagg
     8101 cagaggcctg ggagtgccac gtccattcat tgccaaatat gggtagccgg cccgataaaa
     8161 gctgcgaggc atcttcgtcc gttggctgaa actgcaatgg aatgtgaggc aaaaccggtt
     8221 cagaagttgc gcaaaaataa tgtcgcggaa tatccgcagc cgtgtaacgc tgtgcggttc
     8281 ccgatcccga gagccggtgg cgcatatgga gtgattgcac gacaaaggcg tcccgcatgc
     8341 ggtgcatgac gccggttgga cccatcgcgc tgagccgttt cgcgtaccag gcgaggccgg
     8401 gggggagggc gttcatattg atggacacct tcatgctgat catgcgaaat ccctgacgcg
     8461 agcaggccat tcaagattgt gtagcctgtg aagataggtg tggccggggt tcgtatggcc
     8521 tgagacttca cccgattttc ttctgtaggt tagacccgta agttgaatga cttgctgcat
     8581 aagacgcctg gttttaaaag gggctgaacc gcgtgtgatc ggcgaattac tcgccgataa
     8641 ctggcagacg gatggctgtt cagagcctac cgtgatcggg ccagaagatc aataagagga
     8701 cgtgtttgtg gttgtagaag tacgcagttc attcttgtcg cgaaaacaat gacatcggag
     8761 cggggaagag aagcctgccg tatgggttta cccatgcggc aggcttctcg gcgacacaga
     8821 ggtgcgcctt tgagggcctg gtctcatgca aaacgacggg tgttgagttt acgagagcgg
     8881 gttgctgagt cggccacgag cgccaagacc gaccagtccg atcagaccgg cgccgaacag
     8941 aagcgccgtt gcgggaagtg gaacagcttt cagcgaatcg agtgacccca cgagctgggc
     9001 accattcgag aacaccaatg tccagcgatt gacgttaaac gagttcagat ttggcgccgg
     9061 attgccaggt aaggccacgc tgctgaaggc cgttccggat tcatcggcca agttcaacga
     9121 aaacagtgac ggtgcgagcc ccttcaccag gttcccggcg gtgcccaaat tgaccgagta
     9181 gagggaggtg gatccgctgg tcatggttgt caaattcgtg cccccaccct ggagaggcgc
     9241 cgagtagttt ccgatggaga gattcatctc ggtgacggat gcgttataga cctgagctcc
     9301 cagcccggac ggcaacatgc cggctgtcgc cgagtcgaat gtgtaggtcc cggacaactt
     9361 caatcctgcg ttgaagccat tggcgccttg tccacccggc gtaaataacg atcccgagac
     9421 ttcgctgatg tttccgctga actgatacgt cacgagggcc gcatcggcct tgctgacatg
     9481 cagcaaggaa aggaccgagc tggcactcaa gatgaacagt gacagcgaga gagtgagaaa
     9541 acgattcgtc atggtggctc cttgtgtagg gaatcggttt gtcgcgcgaa gacccggtgg
     9601 gtgctgcgcg aagtcttccc ctcctacgca agtactaagc cattttagcg ggcacgcaag
     9661 ccattgataa ttcaggcgtc aaattcgtga cggtgggaaa tgttgtacgg acctggagac
     9721 ggaccgttca tttttgcccg acaaggactt ggtggagcgg gaagaaataa actctgatgc
     9781 cagaccgggt agaagagttc atcagaatcc cacgtgttca acaaggccat gcaggagaag
     9841 gactggtcgc tctgcggata ctgtctgtgg agagaaaccc ggtgccgggt tgttctttgg
     9901 cgtgaggcga tcgagaacct tagtgacctc ctgaggaaca ggtggtcctg agcagttcct
     9961 caaagcgtcg ggcaagtacc agacgatcgt agtgcgctct cacgtggata cgcccgttgt
    10021 ggccaaaacg tcgacggagg tcctcactct cgcgaagttg aagcaggcgg tcggcaagtt
    10081 cctcggcgtt ttccggctca atgcagtagc cggagtccgc agcacggaca atgtcggcgc
    10141 tctcgccttc gactccgagg ataatcggtt tctccatggc catactctca aaaatctttg
    10201 agggaatgac ggtcttgaag agatcggatt tcttgagcaa gatgagactg acatcggaga
    10261 gcgcccagag ccagggcatc tgttctttcg gttgctgatc cagcatgagg acattgtcga
    10321 gaccaagttc atttcgtaag gccgccaggc gctggcgctc ggcaccgtct ccggcgagaa
    10381 tgagaacgat gtccgggtat tcgcgcagtc gctgcgccgc gtagagtacc gtttcgaggt
    10441 ggtgggccat tccgtgcgtg cccaaatagg cggccacgaa tttcccctga actccaagcg
    10501 attcagccag tctgttcgtt ccggtgaccg gtttatacaa cgtgaggtct gctccatttt
    10561 tgatcacggt gatcttttct ttggggatcc cctttccttc gatgtaccgc tggaacgcat
    10621 ccgtcaccgg cacgattcga tcggcctgac gataggcaaa cagttccagc cattccagga
    10681 gatgtatgat gaagcgattc ttgatggcac ccaccgcaag aatggattcc ggccagagat
    10741 cccggatttc cagcacccag ggggcccgct tcatacggct gacgacatac cctgccagtc
    10801 cgttaaagaa ctggggtgaa gtggagatca cgatatcggc tttctgaatg aacggcgccg
    10861 ccacaatcgc cgccgccata taagagacgt agttcagtgt ccgtttgaga aatccttcat
    10921 tggctgtcac ataggtccag acacgaatga tcgtgatccc atctttctct tcacgcgtat
    10981 acaacctatt gcgatagcca ggatagacct tcccgcgagg gtgattcggt gcacaggtga
    11041 cgacggtcac gcgatgcccg tcccgtaccc actgtcggca atgttcaaat gtacgcgagg
    11101 ccggcgcatt cacttctggc gggaaatagt gtgataggaa aaggatatgc acgggttacc
    11161 tcagtgggaa catgggaaca tccggcgaat cagagaggag tcgaatccat tttcgtcatc
    11221 ccgtagatta tggtcacggg tacggtcccc cgataggtca tgacgagcgt actgttggga
    11281 tactccagac cgaactcagg gaaataccat cccggctcga tggtcaacgc cacgttcggt
    11341 gtcggcgcga tgacgaggat ggcgttgccc ctctcatcac gtatctccgc agaccgcccc
    11401 actaacaaaa gccggtagtc ggggtggaca tgaagcaagc tttcgatcgt atggatgccc
    11461 tccccgttca ctgtgtcgcg aaccgtccac tcgcccggtg atacgtactc aatcactcgt
    11521 gaatgcgtca gccggccgga taacctggca tacccatcgt gagcgccctc gaaggttgca
    11581 cgaacttctc catcccctgt gatgcgtcca tagatcggat aggctcgccg agctacccga
    11641 aagacccccc aggtttcaga ttggtcttgc tgatcgatgc agagcgtatt gtgtgctctg
    11701 gtgcttcgtg cataggtcct gcgccgtccc ggttcgtagt catagactcc gctgtccacg
    11761 atgatgcgtc ggccgttcac agcgagttcg aaactcagcg tatccgcatg ggcgtggccg
    11821 ggctgataat cagggcccac gggtccgcag tcaatgacca tcatgtccga ggcatcgcgg
    11881 atcacatagt agccggtctg tgatttttcg ctggtcgtga ggccggcgga aaaagaccgg
    11941 cgcttgtacg tcatgattcg gttgccatag gtcaggatgt cgccaggtgg cggcgcaatg
    12001 ccgagtgcag cgtcgttgaa cagaggaatg tcgccatcag gcaggcaaat atcatccaga
    12061 aagcgtaagg cctcatggac ccgggtcgtg aaccgctgtt gttcgtcgaa gtgcagggcg
    12121 cgctgcgatc cggtcatcag gttcaggacg tccaaataat ccactaggct gatggcgtga
    12181 tacatcggac ttcgctcata atgtccgcca tctgaaagaa attgttcgtc caactcatcg
    12241 gcaagaatct gtcgtccctt cgccagccag cgggctgcgt cggttccctc aaaatacacg
    12301 ccggcaaaaa acagcgccac gccgtttttg aggtagtgat tggccaggag atggtattcg
    12361 agattctgtt ctaaccactg agcgtgagtg tatacactgt ccagccactc ttcgggcaac
    12421 ctgtcccctt ccttcatgaa gaatttgatc cagttgacga gccgtaggga cacggtgtaa
    12481 ggctcccagg cgtctgtcgc accgggggca ttgtgtgcga tccaatccga aatcagtgtc
    12541 ctcttttcat cctgcgtacg gcccgactcc tgtaggtagt caaagtagtg aagattatat
    12601 cgccagagtt tcgacatgtc tgaacttgcc caatcgacgt tggggcgaga catggctttc
    12661 ctgatattca gaaagcaaaa ttcgtaggtg cccgctgcag cctctctggt aatccaatca
    12721 cttgtcggtc cgaccaggag tcgtcgacga acggcgccat gagatgccgt ccggtgccga
    12781 actggtaaca gccggcgaat gatcaagtat cccaactgcc tggggcgaag gaatcggagt
    12841 gtatgtgcaa accggacgat gcgatgaatc atgccgttgc agagtgatgc gtcatggaac
    12901 agctcatcga agacgatcca tgagttcatg tcgtaggtcg tacacttttt gcgagtccca
    12961 tttctcgagt tcgacggcgg atgggttctg cctgatggga tctccgagga tcacctctgc
    13021 gcttcctcga tccacggccg acaagaggga gtagtcttcc agtccctcac gaaacgcttc
    13081 ccatcggcga ctgctcacga tacggttatc ggcttcatag actaccgccc aatccgggta
    13141 tttcccgtcc aggtcattcc aggctgagct gccgttggtg tcgctataag accagaatcc
    13201 tactccgctt gcgccgaaca tccagcccca ccaggcaaga aatcggtatt gatgaagagg
    13261 agagcccagt ttggccggcg aacgcggatt cccgtacaac caccacggtt tgttcagttg
    13321 agggaaaaac gtatgcgcgt gctcccgtaa aaatatgagg ttgggttgcc agatatcgac
    13381 aagattgttc aggcgttgca gatcgtcgat cgtggtgcgg ccgcttctcc cggacgtcgg
    13441 gtcggcatag aggcgaatgg ctgggtctac gctcttaatc gcgtgggcca cagtctccag
    13501 gaatgtgatg ttcgctcccc ggggctcgtc cacgggatag acggcccact cgctcttgct
    13561 gaaccccttc tgttcgaggt aggcggacat cttttccacc cagtcaatca aacgccgttt
    13621 cgcatcatag gtcagattgg gatgttgggt ggaaaggccc aggggatttt tggccatgtc
    13681 ccatcccaga tagagcaaca acgttccctt tccgcgtgca agggtgagga acttattaaa
    13741 agccgtggca tgctcagcgc tccacatgcc gtccaatccg acacccggaa tgttcctcgg
    13801 atgcaggaca aacacattga ttccatgggc cagcaagtcc tgaaaggcaa cctctggatt
    13861 acgccagatc gggttatcgt tgatataggc ccagttgatg gcggccagct ttcgtacctg
    13921 agggagcgcg gccctaacca gaagttcacc ggttgccgtt gcgacttctc gaccgcttga
    13981 accgagaatc gaaattgtga aggtgtgtgt accgactgaa atagtccgga gatcaaatga
    14041 gatccaggca taattggatt tgtcggtccg aagcgcgagt gtatgctcgg ggccgagcgg
    14101 ttcgagaggg tcgtatacga cctgtccatt cgcggccaca attggtatga cgttgtacag
    14161 tcgaacggat tccgcaggaa ggccggtcac attgagcacg accggcgagg ggcttccgcc
    14221 ggcccatgtc cgtaacccga gacaaatcga ttcttgttca ccttgaaatc cctcgaccat
    14281 gaaccgcttc ggcagggtgt cccgttcctt ggccggaatg tcgagtggaa gatgccccca
    14341 agggtcctgc gcccaccaga ggagctcggt ctcggaagag ggcggtggac tcacggcact
    14401 ctgtgtcacg gcacgagaga gaagattggt gcgaagccct tcaacactgt cggtgagagc
    14461 ctgggttttt gaacccaccg gacgatcttt gggtaaggac ccgctcgcct cgttctcttg
    14521 ccagaccacc tcatccagga acaagaacag gaactctcct ctggcatgaa gcacgatcag
    14581 aagggtgtcg gttgcatagg agacgggcac tgtcagccaa tggtgggctt tgtccgcgaa
    14641 ctgttgagtg tcgggagaaa gacttccgac cttgaggtaa cggccggccg tatccgtcga
    14701 gtagacatcg atctggtgcg ggacatcgac gtccgcatag agtccttttg ccgtatgaat
    14761 gcgcaagctt cctgaatgtg actcacgtga ccgagcctgt ccacggtgaa ggttgagtga
    14821 gaacgtgacc ggactggttg cggtccatcc gacggcctca cgttttgtcc acatagggaa
    14881 attcgtgacg acgccatccg tcaattgaac ggagtcacct cgatcggtgg tcgtggcata
    14941 gtttggttga gaggacatct cccgcacttg gaccgtggtc gtgccggcga acgacgattg
    15001 ggcgacgacg atggtggcaa gcacgaacca agcaggaata gcgtgatgaa tgcgcataag
    15061 ccccctcctc tacttagcct ccgatatgat cgacgatcac gctcatacgg ttcgttgaag
    15121 ccagcgctcc agcgtcgcaa gtccccacag caaggtcgaa ttgtcccgcg tcccacggga
    15181 aaagtcgtgc atctccgtgg caagaaagct gcgatctaaa agagttccaa ttcgagccgt
    15241 cggtccgaga atgaaatcct tcaaggcagg aaggttagcc ttcagcatca cgttgatcgg
    15301 gggattaaaa gggcgtttcg gcgcgttcaa aatttctgcg ggaaggtgtc tctcgaaagg
    15361 gacggtcacc agcgctttct ttcgcgattg cactttcgct gtggtcggca gtcccagaat
    15421 cccatggatc agaggtgtag ccagaaacgg agttcgcaat tccagagagt gcgccatgct
    15481 ggttctatcg gcattctgca acaggttgaa gggcacaaac gatttcatgt caacgatcac
    15541 tgccgcgcgg atcgggtcgg cctgatgccg ccgaaaggtg gcgacgagat cttgatagaa
    15601 accggtctgc acttcgcgcg ttcccgtggc ccaggatagc gtaggcgtgt ccaaatagct
    15661 cgaccaatcg agaaatgcct cctccatggg ttttcccaag ctcttcgcaa attgtttggc
    15721 ccgcctcgca ggaacggcga tccgacctgt ctctggaaac acattcagta actttcgcag
    15781 gccggcatga aacatcggcg gccatgaagc gagggcggca aaatgttcca gtgcatggta
    15841 tcgcggatat ccgaccagca gttcatcacc accatctccg acgagacaga ccttacagga
    15901 ctctgcagcc ttcgccgcaa tcatgtcaga cacgatcgag gtggagttcc cgaacggctg
    15961 atcgaacagg tgaatgacac ggtccagggc gggaaagaat tcgagttgcg tgaagggaat
    16021 agtggtattg acaagtccaa gattcgccgc aatgcgcttg gccgccctgc tttcatcttg
    16081 cgccctgggg tattcaagcg taaacgtcgc cagcggcgat ccggttgaag cgagttgtgc
    16141 aaccgccaat atcgcgttcg agtcgatccc tgcgctcaag aagactccga ccggcacatc
    16201 gctcatgagt cgcaaccgga cactccggga gaatagcgtg tgaaaacggc gggccgcctc
    16261 ggcaaatgtc ataccggggg ccttatcgag actcgactct aaatcatagt accgctgcgc
    16321 cggttgccgt tttcgatcgc acaaatcgac ggtgagatag tgtccagggg gaagttcttc
    16381 gatgccttgg tagagcgtat gcggcggtgc cgtgtacaag aaccgaagat agtcgaacaa
    16441 cccctcgcgg tccagtgacg tatttaggtc ttgtaacgtc aacagacttt ttatttctga
    16501 agcaaaggcg aagcgacggg ttccgttcgt gacggtgaat gaataataga gcggtttttc
    16561 cccgatggga tcacgggcaa gtagaagggt attcgcctgt ttatcggcga aacagaatgc
    16621 aaacatgccc tctagtctgg taagccctgc caccccttcc ctgatcagcc atttcagtaa
    16681 gacctccgtg tcggaggtcg tccgaaacgt gtgcccatcc tgttcgagtt cagcccggag
    16741 ttcagaaaag ttatagatct ccccgttaaa gaccagcaca tagcgttggc aatcagatac
    16801 gaatggctga tggctgcgag gatcaaggtc gagaatactg agccgcacgt gcccgagaaa
    16861 acagtgcatc gcggcgagtg ccgtactccc cgtaaaatcg gggcctcgat ggtgaatttt
    16921 cgcaagcatg gcctggaggc aatgctcagc gtatccgacg attcctgcaa tgccgcacat
    16981 agtacctctc ggtgactgct agagagttct gggtcgtttc acgcatggat ccggaagacg
    17041 tctgttctgt cgaagaacgg tgcgctctac ctattcagca atcgtctact taggtcaaga
    17101 aaagtctgcg tccagatgcg ggaggagaat tgttgcgcca tgagcgccgc gccgtttcgt
    17161 agcgcactca tccgtacgga cgagtgcgta agtgtgcgca tggcgtccgc aagtgccagg
    17221 ctgttccgcg gctcgatgag aatgcccgat cgctcggaga caatctcggg tattccgccc
    17281 catcgagtcg tgataaccgg aaggcctgcg caataggctt caagaatgac tccgggatac
    17341 ccttcacctt tccagtaggt cggcagcagg agtacatcat actggccgag tgtcggaaca
    17401 acggcttcgg gggccaggac acctttatag cagaggtttt tcccggtaag tgtctccgga
    17461 ccgatgtcgg cggacaatgg accatagata tcgatggtga gtccttcatc gagaaggcga
    17521 gcggcctcga taatttcccc gatccccttc tcatgcttga cgtgcccgac atacacaaat
    17581 cgctcggcgc gatcacgagc tcgtgatgaa ggcgcgggaa ttgtcgacgt gcatttgcgg
    17641 ctattggcat accactctac cggctgctcg gtacgttgtc tgaaataatg aacggaggcc
    17701 tgccgttcaa aaagcaaggt gtcggctcga agaacggtcg cctcgaagat tcgcctgctt
    17761 ataggagatg cctgctgatg ccatacatcg tagtctccgc cgaacccccg aaaaatccat
    17821 tttcttcgga aggctcgaca gagaagatgt acgaccaggc caaattgcca tgcaccgccg
    17881 acagaggtat ggaatgacac aacgtcggta aaggggatat gccgaagcat gctcaagagc
    17941 gacttacacg cggtgaacaa gttgctcacc ggagtggatc gggttcgcga ggtatcgatc
    18001 acctgaagtg taatgtcagg acaggcagcc aactcgtcca ccagttgctt gaacaagacg
    18061 gttgttccgc caaccggtgg aggcaaagaa ccgataagca ccatgttcat gtttgtgacc
    18121 gttgaaactg gccactctga atgtcccatc gacatgcttg catggggacg ctccgaacac
    18181 atgggtaagg tgcctgagcg aacagtgtga gggcactgac acgaagaaac ggtctctcat
    18241 gcatcgaaat ggtcacgctg atagtcgcct tatttcccct gtccttcgtc gatacggagt
    18301 gcagatggtg agatcccgtt cactcagtca ggcaaaagat tctcgacgag ggaaattgcc
    18361 gcctggttag gccccacgag gtcgatacag atcctggtat tgcttccagt ttgcatcgag
    18421 agtgagatgc cccagcgcat accgagatgc gcgatcgcgt atcccgtcaa aatctgggtt
    18481 gaagatgaac tgaattacct gttcgggcaa atcttttctt tcatccagca agaggcctac
    18541 tcgctcccgc acgaggtctt cgctatagtc gccgatgcct tgcgtcataa tgaccggaag
    18601 gccacaggca aggtactcgg ccactttgat cggactcgcc acgttattca ccatggtgtc
    18661 gtgccgcatg atgacgccgg catcacaggc ggataggtgg cggcgaacgt cgtcatggga
    18721 gcagttcttt aaatgaaagc gcgacgggta tgtcaacgat gcgatggttg atcggaggtg
    18781 accggcatcg ggggtcagaa tcataaatcg cacatcgtcc cggcgacggg acagctcatc
    18841 gaagagtgta aggatgtcgc tcacccgctg ccatggcgcc gtgccacccg aataacagag
    18901 aagcttttcg tcagctgtca ctctgaattc atcgcgaatc tgttctctgg ctcctgcttc
    18961 aaggtaaaac cgctgtggat cgtagcagga aggaataacg gccgtgcacg tgcgggagac
    19021 ctgtcgctgg atctgaagat tcaggcgctt ggatacggtg gctaaccgtt ccgctctttt
    19081 catttcacgc tcttcgataa aattgatgag gcgaacggca atggtaccac gctgccggct
    19141 cagagcaact tccgatgcca gcagtgcccg gagatcgtaa atagatacgg ccccagaatc
    19201 gctggccaat ctgcgaccga atatcgaagc gatgcaggag cgtgaataca catgtgtggg
    19261 ggcgaatgtg cgcgcgaacg atctgaggcg ggcaaaggtg ttgtaagtcg tcgcggctaa
    19321 gctcaatgca ttcgggcgtg acggaaacag gtcgaacact ttcgcctgga tggagtatcg
    19381 ctgttcgatg aatgcctctg ccctgtgtgc ctgctcctcc gaccgttcgg caccgacaaa
    19441 ctggcagtca aaaccttttc gtgcgagaaa cccggcgacg gcaaacactt gagacgccag
    19501 aactccgctc tccaaagcgg gccactccgg gacaaaaaac agcactctcg tatcaggagt
    19561 tgtcatgaga tccaggtcac agtgaggatc atcgacccgc tgagcgggtc aacattcccg
    19621 ggagttcaat ggccgaggcg agcgctgaat cagatagatg gcagcataga cgccgagcgc
    19681 gagccagaat agtttcacat cttgataggt aacggtcgca atcatgaaac aaaaggcaaa
    19741 taagccggaa agggcgcttt tgctcaattg aatgtcggcc actgcctttg agaacgcctc
    19801 gctcttctgc agccgtcttc cgacacgaat ggccaccgca atgacggtgc cgtacagggc
    19861 aaaaccaacc actccggtct cggcaagcac ctgcaaatag gtattgtgcg cggctccatg
    19921 gtagcgatcg taaaacgcgg acataccgtt gccgaagatc ggcgaatcga accacatatc
    19981 gatcgcctgg cgccagaggt caaaccgtcc ggtcgctccc tcgttgaccg ccccgccgat
    20041 gccgaaaatc cgcttatgga gcgacgggat cgatacgacc gccacagcga atgctgcagc
    20101 catggaccca accgggatga tggctttcac agcattaccc cgtaagacat acaaacctcc
    20161 aacgagggcg gtggccagga aaccgctgcg gctgtacgtt cccacgcacg caatggcagt
    20221 catgcaggca ataccggccc aaaaaagctt ggctatcgcc ttgccggcgc tataaaacgc
    20281 aagcgccaag gccaacggga agacgaggct cgcactcgta tagttgggct cctctccggg
    20341 caggagaaaa cgccgcccgg tttcaccgcc atgccggata tgaattcgat gttcgagacc
    20401 gaattcatcg ttgaggaaca tggcctccat cgttcgatac agaacggtat tgtctccgaa
    20461 ataaatgtga gccgctttga cgatcgtcgt caggccggcg accaccgccc accagatgag
    20521 cacccggcca agatcgttcc ggctttgcac ggtgagaaac agaatggtca gcagtccgat
    20581 accttcaaga tattttttgt aatacgggag ggccaaggtc atgttgtcgg acagaatggt
    20641 ttgcacggct ccgtagagga caaaaaccaa agcggcaaga aggaccgggc ctggtttgat
    20701 caaaagtctc ccgattgagg cgggagtcaa cagaatagtt gacgcgagag tgagggccgc
    20761 gacgacggta aagatgttag ggatccctac cgtaacagca ggcgtataaa aaatcagtag
    20821 cagaattagg agtccgttac atgggttgcg aagcgtgaga atgaccgcca ggcttaggaa
    20881 catggcaggc cccgcaatac ctacactcac gatcgacgag gcgaagacca atatccacag
    20941 cgcacacatc agcgccgcgt cggtaaagcc aatcgtagca tccagaattc cttccggcgg
    21001 gcttgcttcc gtacgttcag atgcgcggtg ctcgctcgtt aataatcgga tatctgtcac
    21061 actcgtagac actagatggt tgggcctttt cggtacacgg ccgtttgtcc caggcagaat
    21121 gccgggttga tacgacacaa gagttcaaaa acttgagatt tgtttgtcgg taacaggtgg
    21181 gttcgggcgg ggctatggtt gcagaatgaa tggtgaatca tttcaagccc agcacttcga
    21241 cctatttgtt caagctcgcc ttttgtgcac tccctatagt ggcctggatt gttgcgattg
    21301 attcgaatcc gctcgtacgg attgatccct tgcagcaggc gtagccgctt cggcagcgca
    21361 gcggcgttgg gagtaaggca cacaagcaat ccattctctg tgaggaggtt ccgtagggcg
    21421 tacaaaacaa gttccggtgc cgtgtagaga tgctcgagaa cctccgcaaa aacgatgaga
    21481 tcgaattgtt gttcagtttc aattccttgt tcttgtgcgt cgttcaagtt gaacgtaatg
    21541 tgattttgcg ggcgtcgttc aagatcgagc cctgcgtatt catgggcata gccgtgttcg
    21601 ccaagcggta ttcccagagt gcacacgctc acgtagtgtt gcgcgagcag gaacgataaa
    21661 taactgcgcc caacgtcaag aacttttgtg tcctgatcgg gcttcaaatt tttgcagaga
    21721 gtaattgtat aagtgaagcg aggcaggcta atgcccacgt agtcaactgt gttgaagcga
    21781 ccatcttcac gttctagtaa ttgctgcacg aatgccctat gagttgtagg tcgctccata
    21841 tctctccgac gaaacccgtc aatcatgctg gttatgacgg gttctccaat tgtaattcgt
    21901 tcgcagtact ctcgcgggaa cgccggcaat aagggcgttc tctatcatat gttcaccaag
    21961 aacgacactc ccgagcccga ccaagttgtt gtctccaatc acggacccag gcccaaacct
    22021 cgctgcggat cctatgtagc aatctgcgcc aatagaaatt gtccgtcgct tcaccccgat
    22081 tccgtgagtc cagaattgac tgccttcccc tgctatccac gagcgcgctc caagtgaaat
    22141 tccacctgtt gcgtcgagct tatgggcgga tgtaatgacg caatcatcac caatgtggca
    22201 aaaacgctca tacttctcat cccgaaaccg ttcttctttc acccaacggc cgcacagaaa
    22261 ctcgttggct cgaccgatcg atgtgcccct ccctatcgac aggtggtagg gtcctatgaa
    22321 tttgtttgcc cgtccaatga ttccggcctc tatatccgct ccttcgacgg caatgatggt
    22381 taagaacccg atctttgctc cgggagcaat gcggtacccg aagagtgagg aatagagcag
    22441 tcgtcttgcg attgcaagcg gcacgatact tactgcaatt gccaatgccg tcttcggatt
    22501 taccatacaa taattcggtc gacctcaccg tatcgagttg taactgcaaa tttattacgc
    22561 gggctgaggt gtgcgcgctc tggtgtatat cgtcacgatc tgttcgcaga catctcgcca
    22621 agtaggcata tcactcactg catcctgcag gtccgaggtg gagggacgct ccatttccct
    22681 ggcgataagg gcgcgaagtt gtgtcggtga gccgggcgga aacgttggta tcaagtgatt
    22741 gatcgagttt gcgatcggca cattttggga gacaagaatt cgacagcctg caactgcagc
    22801 ttcatacaga gtcagtggca tgacttccga cgtgcttggc aacacaaaca actttgcggc
    22861 gcgatatgct gaagccagga tgggatcgtt gtgggcgaat cccccggcaa atatcacgtt
    22921 ctcatccgcc tcctgacggc atctttccat gtagttttct tgtccgggag gaactttccc
    22981 gataatgact aaagaatacg gagtcccttt catggctctt atcaatgtca gctgattttt
    23041 tctttcctcg accgatgcca cgttcagaac gaaaccctta atgccatatc tttgcttaaa
    23101 tagagacgga tcgattgatc cgaactgctt atttaggcca tttggaatgg ttacaatgtt
    23161 gtcggaatca agagagaata tttttgataa ttgagctctt tccggttcat tcaaggcgat
    23221 gatctgccgt gcagcgtgca gcatcatttt tgcgcgtctc agatccgagt acattccggg
    23281 gacaattccg gaggatagcg tctttaggcg cgtcagaaag ggcggggtct gaaacagatt
    23341 caacacggaa gagacgatca ccggtttgcc caatccaatc gaacgttgga catgagagat
    23401 cactgcgcca tcgattccaa atacatgaca caggtcaacc tcggggatct ggtcgcgcca
    23461 aggatcatag aagacgacct caacccctag ttcggtcaac ccttttgctg tctcttcgat
    23521 ctgaatctgg agtccgcccc tattcaagcc gatccgtgga tatgtgagga agagcactct
    23581 catgtgcgat atatctcgtc agacccgcat gccggctagg ccctgctcgg gagggcggac
    23641 atacgttcac ccatacccag gagagtggcg cgcagcacct tgcagtcttc cagcgtgacg
    23701 gctttaaatg ccatcagtag caacagggtg ataccggcaa aaccacataa gagtgcaaaa
    23761 gaagatgcca ggctggctgc ggcaaggagg aacagcagca acggaacggc cgcccgcaag
    23821 aagcccgttg tcaacagatg ccttgtcgag acattatctc cggccaccaa ccttgccccg
    23881 ataaggttcc ctaaaaaggc tgtcaggact aatgccgtcg ctgcgccgaa accttcgtat
    23941 cgcgggacca gcactccact cgcaatcaca atcgtacaga gctgcggtaa cgtgaggacg
    24001 aacgcttttt tagactggcc gttgccgatg agaatgatcg agtagaggta attgatgatc
    24061 gtgaacacca cccatacgct cagggcttgc ataatcacaa tccccgcctc gtacttttcc
    24121 gaatacaaca gcgtcatcag tggctgcgcg agcgcaatga gccctgtcgc cgccacagct
    24181 aggagggcgg tcatcatacg aatgagcgtg atctgaggat tcttgtaagt gctgttctgc
    24241 gcggtaatat accccagcaa cgtcggccga atcgattcaa aggcgcgttg tagcacgctt
    24301 ggcacttgca ttgctgctga atacacggcg agtgcggcgg ttcccatgta ggtggttaaa
    24361 atcgtgtcag acgtgcgaac catgatgact gataccagtg aacatccata caaccatccc
    24421 ccgaacttca ggaaatcggc gctgcgtgga tgacgcagtt ggatggaaaa cagcaagggg
    24481 agcatatacc aaatcacagc tattccgatg attcgtgagc cgatcatccc ccacagtaac
    24541 gagctgatac ctaaccccgc aaagagtccc cccatcgaga agagggcccg gagaacttca
    24601 atgcctacgc tgagagacga gaggactcga aattgctttg caccgaccaa gagggacatg
    24661 acggtctgca tgcccgtggt caggagggcc acaggcgcaa tgtacccgac gtgactgaca
    24721 aaattcggag aaggccataa ggacgtcaag aacggcactg tgagacccat acagagacag
    24781 gcgaggagcg atgtggcgat ggctatcgtc aaaagatatc gagcggtgtg tatgaattcc
    24841 ggagaatctg cctgtaactg ggacagcgtc ttaatcgcgg tgttctggaa gccgatatcg
    24901 ccgaagaggg ctgtgacttg agccacgagc agcagcaaga aaaaggagcc aagttcttct
    24961 tgtgacagca ggcgagccgc taccatgatg gtcagcaatg acgcccccat cccgagtaga
    25021 ttggctcccc ccgagagaac cactccaagt agatactgcg cttttatttt ttccatctaa
    25081 gaactagctg aaagcacgta ttaaggcgct caagtaaggc gccctagtac gtcgattctc
    25141 gcgtgacttg cttgtggtgt tcaagacaac aaatgctcca agtgtcccac gattcgctcg
    25201 gccgtcttcc cgccccattt gggcggaatg gcgcccgtct tccattgccc ggtcatcacg
    25261 cgcgcgaggg cgggagggag tttgcacgga tcggttccga tcaactcgtt ggtcctgatc
    25321 gtgacggttt ccggccgttc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 natggtggtt tggcctttgc tcgtcaacgt cgacgtggac attctgcctc cttacactaa
    25501 cagcgattca ttacattagc gtaacgatag aagaagcaca aggtcattgc cttagcccgg
    25561 actattcatg catatctgct ccggggtccg atcctctcgc ccggcgtggc tgttctcgtt
    25621 cggccttctc aacggactgc gagtacgccc acgtcggcct ggcttgcgag aagccacggc
    25681 ggacttcggt ctcaaacccc tcgcgacggc attcatgagt aatccaagct catgtctctt
    25741 cgagcacttt atccagaggc tagcagcgtt cccaggtgcc ccacgattcg ctccgccgtt
    25801 ttcccgcccc atttgggcgg aatggcgccc gtcttccact gtccggccat cacgcgcgca
    25861 agggccggag ggagtttgcg cggatcggtt ccgatcagtt cgtcggtccc gatcgtcacg
    25921 gtctccggcc gttcggtgtt atcatgaaca gcctggcatc attcggaacc acattcaaga
    25981 acgagcggtg catttcttcg gttcgccggc tagtcaaacg gtaagattcg tgctctggcg
    26041 cgttgggggt caatagagat cagcgccccc tcttcgagtt cccggcgaaa ttgctcgatt
    26101 gcattgatga gcacgactcg aagcgcgtcg ggcagcacgt tctgtgtgcg cacctgcatg
    26161 acgcttgggc cgactgcccg tgttgcggcc agcaatgctg agaaatcaag gtcatgcgtg
    26221 acgattacat aggcattgtc tctggcccat ttcataatga cttcgtcggt cgcgcgatga
    26281 tcgccgatgg tcgaccagtg gatggcgtcc caccctgccg cagtgaacac gcgtagccag
    26341 tcaggcgtaa gattcatgtc gagaagaaac ttcatcagga aaccaatgga aggtctttct
    26401 cctctgaacg ccaggctgcg taggttaatg cttgcaaaat gtcctcccgt tcgatgtagg
    26461 gataggccgc caacacttcc tcaatcgctt ttcccgtcgc cattacaccc acgattgtcc
    26521 cgacggtcac tcgcataccc cggatacaag gtttcccccc catgaccgct gggtccatgg
    26581 tgatacgatt caattctttc atggtgtcct ccattctatt ggagagtcgt gtgtcatgtt
    26641 gcaggcccag gtggcgaccg tcaccgctct tttattgccc cgccagtagg tccttcagat
    26701 gtgccacgat gcgctccgcc gctttcccgt cccatttcgg cggaatcgcg cccttcttcc
    26761 actgtccggc catcacgcgc gcaagggccg gagggagttt gcgcggatcg gttccgatca
    26821 actcgttggt cccgatcgtg acggtctccg gccgttcggt gttatcgcgc aaggtcaggc
    26881 agggcactcc cagtacggtc gtttcttcgg tgatgccgcc tgaatcggta atcaccccgc
    26941 gggcgtgttt gacgagatag ttgaattcaa ggtaacccag cggatcgaca tagtgcatgg
    27001 aaggaagcgt cttaccggct tcacgcaaat tcttggcggt acgcgggtgc accggaaaga
    27061 ccaccggcaa cccgtgagta ccgtctgcaa tggccttcag cagtccaagc aattgctgct
    27121 cgccgtccac attcgcggga cggtgcaggg tcacgacgaa gtaccggttc ggttccagcg
    27181 cgagcgaact ccaacaggcc ggcggacgca aacgcgggag ctgctttaat aacgtgtcga
    27241 tcatggtgtt gccgacgaag aaaatccggt catccgtgat accggcccgg cgcaggttct
    27301 cgttggcggt gtcgctggtc gtgaagaacc aattggtaat ggaatcggtc accacgcggt
    27361 tgatctcctc cggcatcgtc cagtcgttcg agcggatacc gccttccaca tgggccacgg
    27421 gaatgcccat tttgcgcgcc acgatcgaac aggccatggt tgaggtgacg tccccgacga
    27481 ccagacaaag gtcgctcttc tctttcgtca gaacccgttc gtaattcacc ataatgccag
    27541 cggtctgctc ggcctgtgtc cccgacccca cctccagatt gatgtccgga tccggtatgc
    27601 cgagctcttc gaaaaagctt cccgacatgg ctcgatcgta gtgttgtccc gtatggatga
    27661 ggcggaagcg caactcgctg ccgttcgagc gagcggcatt caaggcgtcg atgatcggcg
    27721 cgattttcat gaaattcggc cgggcgccgg cgatgagatc gatacgtttc ataaatctaa
    27781 tcctgtctgg gtttgtgcaa tctgcgattc cgcccttgcc ggcgagacag ggcgacgagg
    27841 ttcaatgctc aagagacggt gagctgaaag acggcccgac tccgatgcac ggccccggct
    27901 gatccgggaa gcacgtggtg aaaccaacgg ggctgccatg gcgaaggcca cctcatcatg
    27961 tacatagcgg agcctgaccc gtcagtcaac aaaaacggtt ctgttcacga gtgggagaga
    28021 attgacaccg aggtgcggtc ctgcgagtcg aaccgttatc cggctgaacg ctcagccagg
    28081 cgaagtggca tgtgcagcga atcatccggg atgaggcttg atctgcgtgg aaaggataca
    28141 gacgcgctcg cctactcttt ttcgaccaag cgccatccct gtttttggac gcagcgctcg
    28201 gtggcctgaa tgatgagcaa ctgaatcccc tgttgtagtt tgggatcgcg cagcgtgtcg
    28261 gcttgacact tattgtaatc cagcgtgaat tgatctttcg ggcggttggg atgcacccat
    28321 tcgctggtac tgcacccggc taacagggag aggaatacga gggagagcgt caggcgcgtg
    28381 caacgagtca tgggtgcctc acaagtgagc gtgtcacaag agaatgagat cgacgacggg
    28441 aagataaact gtggttacgt tgcgcgtcaa gccggggtgc cggcgactga gcgccctagg
    28501 gcaacggtac agaaaacgtg acctcggttc cgcgctcgtt gtaggtgaga tcgggaaaga
    28561 aggccttggc gaggaatacg ccgcgcccgt ttgcgtcgcg actgtcgcag ggttgatccg
    28621 atcgcgtcag gaagcggttc caggtgaagc cgtttccttc atccgtgatc gcataccgca
    28681 tgcggcggtg gaacttgtca tacagcgctc ggacgatgac gcgacgttgg gcaaagcggg
    28741 ggttgcggcg tcgttcagcg atcaggtcat caaagcgatc ggtgctcaac gcctcatgtt
    28801 tttcccgata gaagatttca aggctgccat gttccaccgc attgacaatc agctcgatca
    28861 aggttgtgcg aaggtgcagc cgttgggttt cgggaagccc catagccgta ctctcaatca
    28921 gccaggtcac gcaggattca acgtctccgg gatcggttcc gatgaccaga cgatactcca
    28981 actgctcgat acccgggaca tcgtcgatgg tctgagggat tctccggagc gcccgatcca
    29041 gggccatgcc gagttcttct gcccgggccg gtttgtacag acaatctccc gcgccggctc
    29101 gaacagcttc caggagagcc tgttcattcc cagcccgggt ggtcgcaatg accgccgtcc
    29161 gtgcgtttcg cttgagcgct tcgcggatca gcatcatccc gttcggctca gggagaaaca
    29221 ggtcggtcat gagcacatcc ggcgacgcca tgtccatcat cgccaaggcc acatgcggat
    29281 caggagctgt catgatcgag aggtctcgag tcttcgcctg ttccaggacc atggccagcg
    29341 tttcggcaca gggctccacg accaacagcg ttctggatgc ggcgggacgt gtcacgcagg
    29401 aaatcccttc tcgacgatca tccggagggc ggccgttaat cgttgaactt cggcatggac
    29461 ctgctctccg atcgccgcca tctcgctcac ggccgactgt cgagctgatt cttcaagctg
    29521 cgccgcagcg gccacggccg ggcgagcgca gaactccatg gccgatcctt tcaatttgtg
    29581 cgcttccttg gccagcaccg gaagattctt gtcggcgagg gcggtctgaa ttacagcgag
    29641 tgtttcgggc gaacgctcga ggaacatctc ggccaaggtc agaaataagt cctgatcgcc
    29701 gtcgaaccgt tccatggcag ctgccaggtc catgacgggt tgatcttcca ttatgacttc
    29761 tcctttgccg aacctgagct gtccagcact tcgatgccga ggagcgccac atcgtcgggg
    29821 aaaatgtcgc cccctagcca ttgtcgagcc gtggtcatgg tatctccgat gctgtgcgcc
    29881 aacgtgtgtg tacggtgcct ttccagtgtg gcctgcaaac ggtccctgcc ccacagttcc
    29941 cctgtcggag acggggcttc atagatccca tcactcagga gatacagacg atcccgaggc
    30001 agcaattgtg cggtcgcact gtcgaacgag attgtcgggt cgaacccgag cgggaggctc
    30061 gccgaagata accattgcga ggccccttgc agagaatggc gaaatgcgcc gcagtgcccc
    30121 gctgttgcat acgacaaggt ctgcgacgga agatgcagac gcccgaccca cagggtgaaa
    30181 tattctccgt cctgcgtcag cggaaaccga cggttcgctt ctgtgatgat ggccccgggg
    30241 tcgaaacttc cgacggcttt cgtgagatta tcttcctgca agaaactcat caatgcgacg
    30301 gcgcgcaagg ctgcggacac accgtggccc gacgcatcga gaatatagag accgagcgtt
    30361 tcggaacccc aggcgctgac ctgaaacaag tcgccgccta acgcgagaga cggttggtag
    30421 gcccactgca tgctgattcc cggagccggt ctgcccggga gcggaagctg cgcgcgcacg
    30481 aagtctgcgg ccgattgcag ctcgctttcc agttccgctt gcttcgcgga cagcagccga
    30541 tgagaacgat cgagatcgtc gcgtgtgcgt tcgatttcgc ggaccagcgc attggatcgg
    30601 aggctggcct gcacccgtgc caggacttcc tgcttgctcg ccgctttgct gaggaaatcg
    30661 tcggcgccgc gcgagagccc ctccgcaatt tgttcgggct ggtcgtgcgc ggtcatgagc
    30721 acgacttggc tggaccgtaa ctccccatcc gcacggatcg cctcgcagac gctggggccg
    30781 tccaggcccg gcatcatcca atcgagaatc accagatccg gacgttgtgc gcggatggct
    30841 gccaatccgg agactccatc gccggcttcg atgacacggt agcccagccg tttcagccgc
    30901 ccggccatac tgatacgggt aatctgatcg tcgtcgacga ggaggatgac cggcataggc
    30961 ttggcggcct tcccggcaag agggtcatcc acgatgcgat gcgctggctc agccacgtct
    31021 gctgctcctg atagcggagt tatccggccg cgggaagacg gctccccgca agggcatcct
    31081 gttctgaagt ataaaccggc aggatctggt ggagattcgc cagggtgacg atttctttga
    31141 catagctctg ggggttcagg aggctgacca tgccttgcgt ggcgagaaag ctcttggcca
    31201 acagcgccaa tgtgccgaga gccgagctat ccaaaaatcg aacgccttgc atgtttaaaa
    31261 tgagatgccg acagccgcct tgcttactcc gctccaccgc ggctttgaag gtggtgcgat
    31321 tggcgtagct cagctccccc gtcagatcga gaatgatgga ctgcccgata cggcgttcag
    31381 tgatctgcat atacggctcc tctcccgggc tcagtcgcga ccttaggcgc ttttcttggc
    31441 cagggtcgac acgccggcat cgatcatcgg gatcgtcttg tgcaaattcg ccagttcaat
    31501 gatctggcga accgtcggct gaggataggc cagcgaaagc ttgcgttttt cggctcccaa
    31561 ttgccgatgg ctgatcatca gcaaccctaa tgccgcactg tcgagaaacg cgacctggga
    31621 caggtcgatg acgatttctt cgatgggatc gctgcagacc accttcaacg cctcctgaaa
    31681 cgctttgcgc tgcgtgtagg taaagttgcc ttcgagtctc agggtcgcct tcttgttatg
    31741 aatttcctgt gtgattttca tgcttgctcc tctaggcgta aagatgaacg gtcaggcacg
    31801 ggtgacatgg ttcaacatgg tcgcggcaat ctcgttgagc ggccggacga tctcggcggc
    31861 gccgagcttg atggcttctt tcggcatccc gaagacgccg gtaaacaggt cgtaaatcgg
    31921 ggtagagcaa tgcgaaccgg acgtgtgagg cgatcagaag cccgagactg actatgcgtg
    31981 ccctccccag gaggaagctg atttccgccc tgccataact gggagattgt aggcttgaga
    32041 tgtaacggtg attcctgtgg gtgatcgaac attccacgcc ttacctctta aaccatacga
    32101 acaacatgag atagacgagg taggccgtga ccattgtacc gctgaaagtc cagacgagcg
    32161 cagtacccat tcggcggtgg gtggtcatgc cggtcaccat ggcgcccacg ctcccataac
    32221 gcaggcgatg caggcccata taaagattgt acgcgcctaa tccgacggtc gtgacggcga
    32281 gcaacatatg taccgctaat atcggcacat agagcgacca atactgacta tcggatcctc
    32341 gaaaggactc acgcccgaag aggagttggt tgagcacgta ggcaaccagc cagatgccgg
    32401 tgatcgtgca ccccacgatc atacgatgcg aatgatgaga cacatcgtgg gttttggcgg
    32461 atcgaacacc tgccaggacg atcaggtagg ccgccgtcac gctgaagagg acgagatacc
    32521 agaggattgt cttgaggtcc atggtctact ttttccactc atcaaccaaa tcgatcaaat
    32581 ccgctccctt agtccgagag tacaaccact cctgtgtcgg tttagatgca ttcactcttg
    32641 agcggagggc ggcaagaatt tgtcccaaac gtcctgccgg cggccagaga cctaatttct
    32701 cgggaagcaa gcatcggctg aaagcccata gcggcgctgt cgcctttcat gcatcactgc
    32761 tgtgttgtcc gaccttctag ctcgacgcct gtgtatcagg gaagtggagc cgaggcaccc
    32821 tccttaggca gcaatgcatc gagcgtttct tcgcttgcat ggttctcaag tacgattatt
    32881 tctacccgac gattctttgt ccgcccttca ggggtatcgt tcgttgcgac tggcttagaa
    32941 tccgcgaaac cggtagctga gaggtgtgta atcggcacct cgtagagttc agagaatatt
    33001 ctgacgacca tcacggcgcg tacggcggag agttcccagt tcgaaggaaa ctgtgccgtt
    33061 cggatcggca cattgtccgt atgaccgaga attcgaatgt gtcgacccat ttcgatcaga
    33121 acgttggcca gggccttcag taacggcaat gcctccgaac gcacccgtgc ctccccgctt
    33181 tgaaaaagaa tggattccgg cagagaaatc ataatggcgc catggccggt ttctatgatc
    33241 ttgatctctg cgatgtcccc tttgaggcct ggattgatcc tggtcacgat ttcccgcatt
    33301 cgccggatga cgggatcgtt gatactgtcg gattccggcg gaatcatttt cggtttcgtg
    33361 tcgccgatgc tgaagggcag ccggctggac ggaatgctgt tcaccggatt caacgcggct
    33421 ttgatagagt cactcactgt tcgatactta ccctcgttga ccgacgacac cgaatacatc
    33481 accacaaaga aggcaaacag caatgtgatg aagtccgcgt aggacaccag ccagcgctcg
    33541 tgattttcgt gttcttcgtg tttcttcttc gccattgagg acccgtaatt cgtcgctcgt
    33601 tcagcgtgaa gcgttcggat cgcgtcaggc gatctgtgtc accgccggcg catcactttt
    33661 tgtcttcttt gctccgctca tgcggaggaa ggaaactctc gagtttctct tgtaatagcc
    33721 tggggttttc tccctgcgcc aggccgacaa gtcccagaat gaccatcgtg cgtaagccgg
    33781 cctcttcctt cagtttgaat ttcattttgt tcgcaatcgg cagaaagaac agattggcca
    33841 gaccgacgcc gtacacggtg gcgacgaatg ccaccgcaat accaccgccg agctttgagg
    33901 ggtcggccag gttttccatc acgtggatca atccgagcac ggcgccgaga atgccgacgg
    33961 tgggcgcata tccgccggcg gcttcccaga ctttcgccgc atggacccct tcctcttcat
    34021 ggtgctcgac ttcgatttcc aggatctcct gcaggagctt ggggtcggtg ccatcgacga
    34081 tcagttgaat accttttttg aaaaacgggt cgtgcagatc cttcaacttg ccttccaacg
    34141 ccagcaatcc ctgtttgcgc gacacgttgg ccagatcgag gatctgggtg atcgtgccct
    34201 tgacgtcatg gggaggattg ctgatgacca gcgtggcgga cgtgagggcc ttaatcacca
    34261 cggacagggg attctggacg caacacgctc cgaatgtccc gcctgctacg atgatgaagg
    34321 ccgtcagttg aaggatggaa ctgagatgtc cgccttcgag ggcctggccg ccgataatcg
    34381 atccgagggc gacgacaatg cccagtattg ttgcaatatc cacggtattc gcacctcatg
    34441 ccccatgatc ccgcacccgc acgtactctc tgcatatttc acggctcagc gggcgagagc
    34501 atgccccaag tatttccatt aaacgcgtat cagtagtgag ccggaatgac tattgcgttg
    34561 cgtagtccga tatcgtgggc gtcagagtgg ctcacggttg ttccagcctg ttcgctcctc
    34621 tcttcgagga cacgttcagt cttgcttggt tcggtggtgc atccagtcgt ggatatcgcg
    34681 tcggagaaac cgccaatgct ttccgatttt ggacgccggc agttgtccta ggcgcgcgag
    34741 cttatagacc gtggatttcg gcacgcgcag aaagcgcgcg acatccagca cggttagaat
    34801 ttcgctgtcc gaaggcgatg caccgtcgag ggtcactttg gttgttgctg tgatggcatc
    34861 attcatgacg gatacgatat cggtgggcgc cgtctcaaac tttagttgcc cgttcttagg
    34921 tgatggggtt taatagacac ggtaggacga ttcagcggtt acgagggatg tactgcgctg
    34981 ccggtcgatg gtgcttatgt tttgctccag gcagaccgaa agaagacacg gcgacacata
    35041 cacaacggtg agagaccatt ccttatggcg atgatcgttt caatcggctc aggcaagggc
    35101 ggtgtaggaa aaagcgtgat tgctgccaat ctgtccatgt tattggccaa acaaggcaag
    35161 cgggtggttt tggcggatct ggacgtgggt ggagcggatg cccatattct gttcggcatg
    35221 ctcaaccctc cccgcacctt gacggatttt gtagaacggc gggttgaacg tttggatgac
    35281 gtcctccagc aggtctcggc acaccccttt ctccagttga ttcccggcac aggcgatacc
    35341 ctggcgacgg ccaatcttcc ctatgccaaa aaaaagcggt tgattcgcca tttccggcaa
    35401 ctgcaggccg atgtcatcat tgtcgatatc ggcgcaggca ctagttatca tgcgctggac
    35461 ttttttctga tggcggacca ctatctgaca gtggccactc cggatccgat gtcggtgctc
    35521 gacctctacc gatttatcaa gctggcagcg attcgtcgtg tcttgtcggc cttcctgtcg
    35581 cgcgatgccg tcacagagac cctctccgat cgggatttct gcagcatcga tgaggtcatc
    35641 caggcggtca gcgaaaccga tcccaatgcc aaggagaccg ccagccgaac actccaggga
    35701 tttcacccct acctgatcgt gaatcgggtg tccgggaagt cgcgtgtgaa tgtgctgcag
    35761 ttgaagaagc tgttgcagga atatgtcggc ggggatctga tgatgctggg ggagattccg
    35821 gatgatccgg cgatgacacg tgcagtgcgt agttatcttc cggtcgtcga atttgagccc
    35881 acggcgcccg catccctggc tctcgagaaa gccgctcaga ctctccttac tctcctggct
    35941 cacccggaaa cgactcaggc tgaaagcgct ccctcacctg aacaggcggc ataggccaca
    36001 gcattcgccg gatccaggtt cctcgcggag agccgcgatc cgacaggtta gctggattat
    36061 ttatggatgt cgtcgcgagg cgttcgagac ggaagcccgc cgaggctgct tgcacgtaag
    36121 gtcgacgcag gcgtatttgc agttcgtcga gggcgcacag cgtgatgact aaagagcgcc
    36181 acgtctgtgc gcgccgccga gccggggagg cggccgggcg gctgaacgag aacagcctcg
    36241 ccgggcgaca ggatcggacg ccggagcagg tattcatgaa tagtccgggt tagtgcgcgg
    36301 attttctcgc ccgcagacgt tcggcttgca cctgcagaat atgccggata atcagctcct
    36361 gctcgtcccc gcccaactcc ataaactgtg tggcgacatg atagcccgtc gagtccctct
    36421 cggccggagt cacccggatg atgcgcgccg tggcctcgat catgctgaat ggaggcagga
    36481 tcaccttcag caccaacagg tcctctgcct gaaactgacg cggggtatcg aagcccagcc
    36541 cgccggcgct gatatcgaca tcggtcagcc gtgcgatggc gacgctcccg ggatgacttt
    36601 tcacgaggga cttgagaatc gtctccagca tccagtcgat tttggagacc cagggtaata
    36661 acggcgagcc tgcaaacgtc tcccctgccc ggaccagcag atcaatcgtc ggtttcgaca
    36721 cggccgtggc gatcgtatct tctgtcgccg gagggagatg ggcgttcatg gcttcggccg
    36781 gttcgtcgaa acgacggtac tcgagtagca agcgatcttc gatgcgcaac cattcgcgac
    36841 gctcttcatt cttgctatcg gtgtgggggg cctgacgaaa ccggtcatgt gacggagtca
    36901 taccgaacct ctgcggtgaa ggaaaacgac cgaggcatgt cgcccgggct caagcgcgtc
    36961 aggcttcacg atcgatcgtg acacgttcgg aagccagttg tccgctactt tcacgtaatt
    37021 ggcgcaggtg tcgtttcact tgagcggcat cggccaattg gccggatatt cctgcctgcg
    37081 ccaccaggat ggccgcgcga acttcttcat ccatgcgcag cagttgttgc gcgactgtgc
    37141 gatcgagttc gcaggccgcc agcctgattc cgcgcacgct atagcaggcg tcgacttgat
    37201 cccagtcgcc ggcgcgggcg gcctccagag cttgaatggt gagcagttcg atggagcggc
    37261 aatcgtggag aggaccggta tcaagtgaac tagctgccga catgcgccac cgcttcttgc
    37321 ctcgcaacaa cgtgccaacc ttctcgtagt gtcgtcaggc agcgaatcgg gccgtccagg
    37381 tgcttgccgt tgtgccgaag gttggcctgg atacactgct gcaccatgta gtcatacaat
    37441 cggtgcaacg agaccgcgat ctccccgcct cgctcgaagt ccaggacgct ggacagttcc
    37501 ccgacaatcg cgatactgcg atcgagaaaa cgagccttgt ccttgtagtt gttcgtcaga
    37561 atggcttccc gggccagctc catcgattga atcgcggaat cgtacagcag cacgatcagt
    37621 tgcacccggc tggcggtcat gacttgagtc tgttgatagg cgttcgcggc ggtggcgatc
    37681 ataatgacct tataacttat ctgagcgcct gaagggcgct ggtttgtgat tgtagtttct
    37741 gtaacaatcc atccaatgag gcgaattgaa gtcgcagccg ctcctcatat tgcgaggcga
    37801 tatcttcctt gcggccgatt tgatcggtga gtctgctgat ctggctggtg atggagtttt
    37861 ttcgaaccgt gaaggcgccg ctgtcgacgc tgtcgaggaa atcgaccgct ttcacgatac
    37921 ggtctgcaat gccgctcgag gtcgtctgcg tcacaaacaa ggcccgtgtg gcgacatagt
    37981 tgctcgccat cgccgagtct aacttggcgt catcaatagt gagtgtcccg tctcgttccg
    38041 ttttaaatcc gaccgcgcca agcgacttat aggtggagag ttcactgacc tctccggaga
    38101 tggccgtacg caactggctc aagacgtttt tcgccgtgct ctcgttaaag aagatcccgc
    38161 cggtcttcgt cgtgatgtcg tacccggtcc gatcgttgac gaacttcacg atgtcgttat
    38221 aggcggtcac cagggttttg atattggtct tgacgctcgc ggggtcgttg ctgatattca
    38281 ccgtgaccgg ttccggatcg ggagtcgtga cggttttcga tttgagcgtg agggtgacgt
    38341 ccggaatcgc atcggagatc acattgctgg cccgctcaat cgaaatcgtc gtctggtcgg
    38401 gatcaccgat cacgacgatg gcattctgcc ctgcctgcag cgtatcgttg ccgcccgtcc
    38461 cgctcgtgtt cgtgaaatca agagtggtcg tatcggtcac cacggtgatg gtattggccg
    38521 cgcccgatgc ggttgcggtt aacgtcagtc gataggccgg gcttgcttcc gttcccgtat
    38581 tgatcacgga ggcggtcaca ccggctccca gatcgttgat cgcagaacgg agctcgtcga
    38641 gtgtcgcgcc ggcgctgagg gtgaccgttt gatccgttcc gcttccgacc cgaaaggtaa
    38701 aggtgccggc gccgctcgag actacttcag tggtgccgga gaccgccttg acggctttgt
    38761 tcgtaatttg attggctttg gccagctgag tgacttgcac cgtataactg ccggtcgctg
    38821 ctcccgcgcc ggcttgagcc gtgagaatgt tgtcatcgct gacggtgctg gtagatcgat
    38881 tgaagctgct gggaagccgg agggcattcg cggcgctctg aagcgcgagc agcttgttgc
    38941 cgagggcgcc ataatcggtg agtttggtct gcagatcctt tttcttcgcc gtcaatgcgt
    39001 cgatgggcag gcgttggact ttggccaatt cggagacgac ttgaccaaag tccacaccat
    39061 ttcctaaccc gccgaaacta atcgccatga catccctttc atgtcgtgaa gcaggttaca
    39121 ccttgtggtg caggagcagc ccggtcggcg tctccaacct cttcgcaaga tcgatgactt
    39181 cctgctgcgg aatctggcgg attatggtgc cggattcccc atcaaggatc ttcaccacaa
    39241 ctcgatcaag gtccgggtca atcgaaaact caactctgga atcagccatc tggaatacct
    39301 cacgcactcg agtgagcgcc tggtcgagtt ccttcttatt gacctcaccc cctgaatgct
    39361 caggcatgga aacttgtttc tccggactcc gtacaggggg atactgagga acagtctgac
    39421 tcgcatgggt agcggctgcg aggaggtcct tgtgattcga aacctggtgg accatgttac
    39481 cctcctaggc tcagtccgtg gataccccgc tcttacaagc ggggtatcca cgggtgatac
    39541 cttatcctcg cagcagcgtc agcgcttgct gtggcaacga attggcctga gccaaaatcg
    39601 cgatgccgga ttgggtaagg atctggttct tcgtgaacac agatgtttca tgcgcaatgt
    39661 ccgcatcacg aatacgggat tccgccgcgg tgaaattctc agccgtgacc gtcaggttct
    39721 ggatcgcgac ctcgaagcgg ctctgaatcg acccgtaatt cgaacgggcg gttgccactg
    39781 aactgatggc tccgtcgatt gtggcgagca ctgcctgtgc tgccgcagcc gtcgacacgc
    39841 tcgcccccgt caaaccgacc gatgcaacat ccaagtcggt caacgcgacc gacaacgagt
    39901 tgcctgatcc gctcttgaaa ccaatgaaca catcgaacgt attgctgctc ccgctcagga
    39961 gagcctgccc gttaaattcc gtcgttacgg aaatacggtc gatttctgat cgtaatgcca
    40021 caaattcctg atccagatag gaccgctcgg tcgatcccag cgtcccgctg gcagactgag
    40081 tcgccagctc ccgcatacgt gccaacaggc tgccgatcga ggaagctgcg ccgtcggcga
    40141 tctgagtcaa gctgatgcca tcactggcgt ttcggttcgc ctggttgatg ctacggattt
    40201 gcgcccgcaa tgtttccgac acgcccaacc ctgccgcgtc atccgacgca cgggtaatcc
    40261 gtaaccccga cgacaaccgc tcaactgacc gccccaattg ggcctgattg attccgaggt
    40321 ttcgctgtgc cgccaaagac gcaacgttgg tgttgactac taatgccatg acgtacttcc
    40381 tcctgaagct tccgcactac tcttcgagat tcgccagcgt ccgtgccgac gaggtgataa
    40441 ccaggtttgg tgaagatgtg gccacatccc cgctgcgcct tgctaccgta ttgctgtgcc
    40501 ccttatcggt aggctgtcgg gaagacttaa gagcgccatg aaaggcagaa aacgggtgca
    40561 atgggaggca tcatccgttg aggtgtccta tcggagagca atgagcacta agcagcctga
    40621 ctggattcag tgagcagggg taataattca tactccagca gatccgcgac ccggatgggg
    40681 tcggcgaagg ttcgcgcgcc caggagttct tgtatccacg ggaggagcgg cgcggatgac
    40741 agtcctgcga caccctgtgc ctgaccggct tccagcattt cggtataatc tgcaagacgg
    40801 cccagccagg catcgatggt tcccacctgc ccggttcccg agcggagcgg gccgagcagg
    40861 accaggccat cggctcgcaa ctgcgcggca aaccgatcga tggcctgctt cgcatctgta
    40921 atgatggcgt gcagcgattg agatctcccg gccacctcac ccacctccgg accgcattga
    40981 ttcaaaaagg ccggatccaa atcgcgatcg ttgatggccc tcccgccgac cgtcagcgac
    41041 gtcacgatgc gatgttgggc atgggctttg tcgctgagaa acgccagggc gttcatgaga
    41101 gacgtgtcat ccggtagttg ccactgttcg ccgtcgaggg tgatatgcat gatcgggact
    41161 cctttggccg agtgtcaaac gtacatctgc tgtcgctacc cgataggcac catgggaata
    41221 gccgtcctgg ttccacgcgg tgcgaacccc tgcggtgtct tgaaagcacg gaaaccggcc
    41281 gcgaccgtat gcagcctgtt ctgccagcgt cgataggtgg caagacgggc ttcggccatg
    41341 ccggtcagtt cgtggccgtc gtcattgtgg gtatcccgca ccagggccac ggccagggat
    41401 gcggtcgccg gggattggag actgagcgct acaatggtct ggcgctccct gttttcctcg
    41461 agttgcatgt gctgcaaagc cctgacgggc aacggccgtc tggcagtcaa acgcaccagt
    41521 tccttcgctc gtgagaccaa cccggctgcg agagggagaa gccgatccag gagtgccata
    41581 ctctctttcc agtcgggcgg aggctccaga accatgggga ccagactggg acgaccagta
    41641 aattgttcga accagaaccg acgccagaag cggccatacc agttgaccgt cggatcttcc
    41701 cgtcccgcgc aaatatcggc gcttcccagg ccgtcctcat ctacccggga ccgatagatc
    41761 ttgatgccgg ttactgtttc tggaaatgcg gctccagtga ggacatgcag gcacagcgcg
    41821 gcaatctgat cgggtaccag ccgatcctta caggcgtggt gcagacagac ctgatcgaat
    41881 ccacaaggcg cgcaccccat atccggctga acgatccaat gccccggtcc atagggaccc
    41941 gtctcgcgaa agtcgacatg cccgactgag agatcgatca ccggagtgcc cactcctact
    42001 gcgaggtgca tggggccggt gtcgttggtc aacaggagtc ggcactgggc cagcaccgca
    42061 gcgagctgag gcagcgtggt ccgtccgacc gcgtcgcaga gaggcccgcg tccgccggct
    42121 tgccgatagg ccatctgtgc ggtgtcaatc gccttccgct cgctctccgt cccgataaag
    42181 acatatccga cgggtgtctg ccgactcagc gcagccaggg tctgcccgaa gagttccggt
    42241 cgccaggctt tcataggatc gctggccccg acttgcaccg caatccaggg catccgttgt
    42301 ccgtcgtacg gtgcaaaaaa atcacgtgcc cattgcgccg tgccgggagg aatgtcgagc
    42361 gacaagggtg caaagggtcc ggtaccgctg ccgcccaagg cgtagatatc taccagattg
    42421 aagtgattaa actgccgttg actgtgcaca tcggtcaaat aggccatcca cgggttatgg
    42481 atggccacgt ccccgtcttt cggagccgcg atgcctcgaa tctctttggc gccgacgtag
    42541 gaggccagca gtccgctccg gcgattgaag gtcaaattta cgacgcggtc gtaccgggcc
    42601 tcgattaacg gcgccgccca gcctgtcatc tcacggtaga gggtcacgac atccttcgtt
    42661 tgtgcgcgac tctcgtcgac caggcgatga aagtcatact cgatgatgtg tcggagatga
    42721 ggcaggagtt tcgcgatcgg agcgaagcgt cgatccacga ccagatcgac ggcggctcct
    42781 ggccactcat actggaggcg ctgcagcaac gtgcccattt gcacgagatc gcccatccgg
    42841 gtgatattga tgagaagcac ttgcttgctc ataacagatc gcgtgtctcg ctccggtatt
    42901 catccaacat cagcaccatc aattcttccc gcttgagtgt cacggtcgag nnnnnnnnnn
    42961 nnnnnnnnnn nnnnnnnnnn nnnnnnaact caacccgctg tccggcagga aaggccttga
    43021 gcagcgattc cagcggcgca tctccggcgc aacgcgagag cagggcttct tgctgtcggt
    43081 ctcctcgcag cacggccccg actcgatcgg gctgagacaa tccgatgtgt cccaacaagt
    43141 cccgcatccg atgcacatag gtatgcgcgc ccaagacacg tgtgcgggcg gcagcggcca
    43201 tggcttgccg agccaccgga tctccgagcc attgccccac gagtccggga atgtcatcga
    43261 agttccggaa cgagactaac tcccggtcgg tgaaaaattc cggcaactgg gagcgagaat
    43321 cgacgaactg aaaggcgccg caggctgcca gttcaaaggt ccgtggattg acgaaatcag
    43381 cttgcggatc aaggcccgcg ccggtcgtgg aatggacatt caaattgatc gccgtggcgt
    43441 tgaacacctt catgcaggtg tccgtatcga tccgtgcgcc gttgagttgg aggacggatt
    43501 ggagctcgtc ggctccgtcc cactcgttcc cccagagttt gaacgaccag ggtcggcgca
    43561 acagtgccgg gaacagacgc cggcgattcg gataccctgc gccgacgaag gagacatccg
    43621 caccataggt gcgccgttcc tcatccgaca gatccatcgg gcgatgcagc tcggggtcgg
    43681 ccgccatggg cagaaagctg acctgccgag cacccgcctg ccggaaggca tcgagacagg
    43741 ccccccgttg aaacacgaac cagtagtcat accccggcgc catctgttgc caataggtca
    43801 agtggcgata gttctccacg aaccacatgg cggtcagcaa cttcttcttg cggagatgtt
    43861 cgagcaccgg caacgtcaac ggggcctgag ccagcgacaa gacgaggtcg ggaggtgact
    43921 cggccagaga ggccaaggtc cattggctca agacttcggc catgcgacct tgcatcagtt
    43981 ggcgattgcg cgcatctttc agtgtcccca tcgcctcata acttgacgca tgcacactgt
    44041 gatcgatcca gtgcaccttg tgcccgaggg tttccagagc ccgtctcaca taacgcgcaa
    44101 tcggcagcga accgccgtaa atgggcccca ccagcgcaat cgtcagctgt ccacccgcct
    44161 gtcgcgcgac tgcgcgacgt aaggcttcct cgaacgcact gtggaagtct gaatccgtgt
    44221 gtgtggccgg agcataggac agacagcgca gggcgcggcc cgtcttgctc agccggtcag
    44281 cgatctcgac cggcgttccg gagagccaga gaatgtggtc ggtccatgat ccgaggggcc
    44341 gtgcgacgag cgcatcatga aattcgttga gatcggagat cagcaccgcg a
//
LOCUS       DAORRQ010000036        44087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_53_length_44087_cov_107.344545, whole genome shotgun sequence.
ACCESSION   DAORRQ010000036 DAORRQ010000000
VERSION     DAORRQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 44087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44087
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53_length_44087_cov_107.344545"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..307)
                     /locus_tag="PK224_12335"
     CDS             complement(50..307)
                     /locus_tag="PK224_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16572.1"
                     /translation="MDSQMMTGNLAAAWGARLADVDYIPAFPITPQTEIVESLAKWCE
                     GGELAARFVTMDSEHSMMTAAGAAEATGARVFTATSSQGLQ"
     gene            complement(292..1209)
                     /locus_tag="PK224_12340"
     CDS             complement(292..1209)
                     /locus_tag="PK224_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRB16573.1"
                     /translation="MYAVRFHGRGGQGAKTASRILGTAAFLSGYQAQDSPIYGAERRG
                     APVAAFTRFGREPIRERGVIAHPDLVVVADASLLEDPAARVADGITDQTILFVNSPLQ
                     AEQIRTQSGLPGRITTLGVTEIALQHLGQREAISALLGAVAARLSGLQQEPLRQAIAR
                     ELEDLGLAPPIIEGNQTVALTCYGSVQAMARESATEPATIATTFHTPVYESPSRGTAR
                     ISATGNSVLRETGGWRTFRPVLVADKCNGCWLCFVYCPDGVISMNKDDRPVIDYAHCK
                     GCQICVHECPTHALVAEREQEGGVAWTAK"
     gene            complement(1211..1519)
                     /locus_tag="PK224_12345"
     CDS             complement(1211..1519)
                     /locus_tag="PK224_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRB16574.1"
                     /translation="MADHHDSCSGLGVSIAFLSGAMLGAMAAILYAPSSGEETRRAIK
                     GYARRTEDEVLEKAKEIRADLSHTVDEAKRYLKETEATIAAALAAGKEAFKKEKTDRA
                     "
     gene            complement(1841..2275)
                     /gene="trxC"
                     /locus_tag="PK224_12350"
     CDS             complement(1841..2275)
                     /gene="trxC"
                     /locus_tag="PK224_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HRB16575.1"
                     /translation="MTEMLHLVCPHCRSINRVPAVRLAEQPNCGQCHAPLFTGHPIAL
                     TAADFDLHAQRAEIPLVVDFWAPWCGPCRMMAPAYEQAAQMLEPQVRLAKVNTEEEQS
                     LAARFGISSIPTLVLLRGGRELARQPGALGLQDIVRWVRSKI"
     gene            complement(2309..2869)
                     /locus_tag="PK224_12355"
     CDS             complement(2309..2869)
                     /locus_tag="PK224_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB16576.1"
                     /translation="MQRLLSIILTIMAAAVVTVVPAVAQHMQPPRVPIEHLEEARALK
                     SPLPDSPDVVEKGKALYHGKGTCANCHGPEGAGDGPVASQLNPSPRNFQHPGFWRHRT
                     EGEIFWVIKNGSPGTSMVGFAGQLTDEEMWAVIQYERTFAEGHGHGRGTGPPGGMGHR
                     GAREGMGGMGHRGAGSGGCEGEHCDR"
     gene            complement(2869..3939)
                     /gene="ppk2"
                     /locus_tag="PK224_12360"
     CDS             complement(2869..3939)
                     /gene="ppk2"
                     /locus_tag="PK224_12360"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRB16577.1"
                     /translation="MASDEQAGRKAKGKKGRMAAGAAVSIAEHNGRLETDDLEIIPTA
                     VPREGDGYQAPDQATGGVTAGLTGLIGEVDRTLTEDDLRRVNTRRGLLQLIKSKNIDL
                     DDVRKTLLYEQELQQLQVELVRLQRWVQADGQRIAILVEGRDAAGKGGTIRRFTEHLN
                     PRAMRVVALPKPTDDERGQWYFQRYIRQLPNKGEIVFFDRSWYNRAVVEPVMGFCSKK
                     EHQRFLQQVTEFEHMLYEDGVTIIKFWFSISKEEQASRFEARRQNPLKQWKLSPVDEK
                     AQELWDSYTRYKEEMFSKTHTTFSPWIIVKANDKQAARLESLRHVLNLLAYKGKDEAQ
                     IRLTPDPNVITRFHRKMVELDF"
     gene            complement(3975..4334)
                     /locus_tag="PK224_12365"
     CDS             complement(3975..4334)
                     /locus_tag="PK224_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRB16578.1"
                     /translation="MATFIISGSRGTDDPTMATLPLMAAKTAKEQGHDVVLWLWNEAV
                     TSARKGSADHVVGVNLTPLKDLLAAVQAANIPIWVCGACAVARQIGQADLIAGAVIKG
                     MPDYIKAVAERDRTVAF"
     gene            complement(4422..5837)
                     /locus_tag="PK224_12370"
     CDS             complement(4422..5837)
                     /locus_tag="PK224_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB16579.1"
                     /translation="MGGRRTVVVGCALVMLVGLFSFPRLLLGSDQTRVKEMIQNNCAG
                     CHRLEGKADSRFNLKAPDLIWAGSKYQRSWLLRYLTGKEAPLYPKGYRWDLSEGPTRH
                     PVVSEDEALGIAEYFEQHNKDPRVKVGAFDLSKVSKFDATFGGMAYKAHACLGCHLVE
                     ENGKLIGGPQSASLVAAGQRYDKDWLFRFGQNPQDFTVHNGEFLADATEPQLRAVIGY
                     LMVQGVKDFKYYEPWTAPEFGMASADRGKVLYKEYCAQCHGFTGKGDGPAASGLEPKP
                     AIHANIPFDKVPTDYLYNVINHGGAAMGKSPSMPYWGLTIGQQGVADVMAYLKVTFKG
                     QAEVAQAAAGSGEGPSGVCPQPRKTAKAPAEFLSKANPLPQSDATIKAGKTLFLQTAQ
                     PVACAMCHGDKGNGQGFMGAALIPPPRNFTCGSMMKDLPDGQLFWIIKNGSAGTGMMS
                     FAGLPDEQVWQLIAYVRSLAK"
     gene            complement(5874..6548)
                     /locus_tag="PK224_12375"
     CDS             complement(5874..6548)
                     /locus_tag="PK224_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB16580.1"
                     /translation="MERMGSPVRHGGGMTQSTAKKTSGFVLAALLCTLLGWVVITAAQ
                     EDDRGTIQPAQARQALTLIHARCAVCHTTDLIVQQRLPADRWTVTVEKMMHWGADLSK
                     DEAAAVLQFVTARYHSGAPDHLPSIEEELAMIGPAVGPRQGSEGPVSGVASRGAGLYA
                     HNCQACHGEGASGGAGPKLARNTILKNEGAFWETVLHGRGPMPSWGAVLSHQEIADIH
                     AWLATR"
     gene            complement(6506..7687)
                     /locus_tag="PK224_12380"
     CDS             complement(6506..7687)
                     /locus_tag="PK224_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HRB16581.1"
                     /translation="MTVSRREWLRTVLQGIGIGMVVGPGGTSIATAQGSEAGQGTGPL
                     TVRVTRPFDAETPVREFASWLTPNERFFTRSHFGPPSAETVQPDVWRLTVKGLVKEEL
                     TLNLKDLQQLEAVTLTAVLQCSGNGRAHHRPKVPGVQWERGAVGNAQWTGVRLRDVLQ
                     RAGVKPQGVHVQLQGADRPALATVPLFTRSIPLAKALHPDTLLAYEMNGRPLPLLHGA
                     PLRVITPGWMAESCMKWLTEITVRADETPGYYMQQAYRIPETAVRLSSGLPGSAMVPV
                     EQMPVKSLIAAPAEGDTVRIGPVTIQGVAWAGEAPVVKVEVSCDDGKTWEQARLLGEG
                     QPYAWRQWQVVWNARTPGPTAILCRATDAQGEQQPATSPWNPGGFLWNGWDRLSVTVA
                     A"
     gene            complement(7684..8121)
                     /locus_tag="PK224_12385"
     CDS             complement(7684..8121)
                     /locus_tag="PK224_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRB16582.1"
                     /translation="MKLSVAYQGGARYDILSDRHRIVTDQPVEDGGADAGMSPVELFV
                     GSVASCVGYFVGQFCARHDISREGLKVDAEWTIAEAPHRVGQIQLAIRLPHRVTPELR
                     ERLLKVAHGCTVHQSIVVPAAIAIELNPHGHPATASDLMEEKS"
     gene            complement(8221..9066)
                     /locus_tag="PK224_12390"
     CDS             complement(8221..9066)
                     /locus_tag="PK224_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB16583.1"
                     /translation="MKHLLFSAIMIVAFMAGCVDSKETRTGAQSAMSQAEPDRLDALF
                     SHPSPDSIPGGQRGEQIRLGYELVVHTQEFAAPYVGNRLTCANCHLDAGLDPNSASYV
                     GLSRAYPEYRARTGRVVTLADRINECFERSLNGKPIPSDSHKLRAIVDYIEWLSKDVP
                     AGSRMAWRGIPRIQAPKPPDVANGAKVFTSRCAFCHGADGQGTMAAPAVWGPQSYNIG
                     AEMARIPVAASFIKSNMPRTRGWALSDQDAYDVAAYINSKPRPDFPDKRNDWPKGGKP
                     ADAPY"
     gene            complement(9579..10163)
                     /locus_tag="PK224_12395"
     CDS             complement(9579..10163)
                     /locus_tag="PK224_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB16584.1"
                     /translation="MSITQKLAGGLVVAVALLGGWGPSFAGAPSAGSVPVEAVMDYLH
                     SVLEANRTFYTVHVVERMQRRGVIESSEKWRELSALPLPAQFFQEASSLATLTGSAVR
                     YRLISLNPINKQNGPHGEFERKALEAVMAHPEQPYKGSVTEGGVRSFQALYADLAVSS
                     VCVSCHNADPRSPKRDYKMRDVLGAVLISIPISE"
     gene            complement(10334..10705)
                     /locus_tag="PK224_12400"
     CDS             complement(10334..10705)
                     /locus_tag="PK224_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB16585.1"
                     /translation="MDDAGRRATVAGGGLLLMVANIVMGGAAGGAEGKANPEAGKKIY
                     LESCQNCHGPTGKGDSDMAAYLSPPPANLTAKGTQSKTDAQLRKVILEGRPGTAMTGY
                     EGAFEEPQLSDLIAYIRSLKS"
     gene            complement(10727..11410)
                     /locus_tag="PK224_12405"
     CDS             complement(10727..11410)
                     /locus_tag="PK224_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB16586.1"
                     /translation="MMTDVWRSGGLLVALVVGMTTVFAVAVTRVAAKGEQAGEQVVRS
                     TCTGCHRIEGPVAARKTKKAPDLIWAGNKYQPAWLVNWLQNPDFKLYPVGYDFRPDRK
                     KRHLALSLDQAKAAAAFLATRKDPRIKEGVMKAGTAEQVARGRKLYQEHGCQNCHYTP
                     AGTPKGYVGGTSSTSFIKLSERLNADWVYRFNLNPNDFEPDSGAYIPKPPLPDEDIYA
                     ITAYMMTFK"
     gene            complement(11447..12772)
                     /locus_tag="PK224_12410"
     CDS             complement(11447..12772)
                     /locus_tag="PK224_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRB16587.1"
                     /translation="MSSRLYDWFDRRLNLKPVQRTLLDEPIPGGASWIYVFGSVTLFL
                     FVLQAATGMFLALYYAPTPDHAYDSVRFIETEVTFGWFVRGLHHWGASSMVVAIGLHM
                     LQTFLYGAYKKPREVMWMVGVVLFLVVMTFAFTGYLLPWDQTAYWATQIGINMVGTVP
                     LIGDFLSRVLRGGEILGALTLSRFFAIHVLFLPAILIGGIALHLFVLRRVGPAGPWTD
                     DRASLGSETFAPRQVYMDAVVIALVFLVVAALALSIPLPLTDKANPSDTSFVPVPEWY
                     FLFYYELLKYVHGPLEPLATWVLPLSVILIMLCWPFIDRNPVRNPIRRPVALGSGLLF
                     LFVVFGLLGISLKNLYAVPRTDPAVVQGRAIYAQLGCAGCHRIHGEGGAVAPDLSTIG
                     DTRPDRAWHLKHFRDPQSVSPGSFMPKFPLTDQQLNDLTSYMLSLKRES"
     gene            complement(12809..13360)
                     /locus_tag="PK224_12415"
     CDS             complement(12809..13360)
                     /locus_tag="PK224_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRB16588.1"
                     /translation="MSYSDDTQSCEGAGLSTPVGSRRTFFHWVTVAAAAVVGVGLAVP
                     LLGSLVSPAFMRRKREWVDVGPVDTLPAGRPTQLDHVTTVRDGWLEAKSQKAVWAVKQ
                     AQGEVKVFSPICTHLGCGYRWDDTEKKFLCPCHGSAYDINGQVLGGPAPRPLDVLPSK
                     VEGGRLLVMYQDFKSGLPQSVEL"
     gene            complement(13450..13893)
                     /locus_tag="PK224_12420"
     CDS             complement(13450..13893)
                     /locus_tag="PK224_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB16589.1"
                     /translation="MTTMARPGVPVGGFKTVGQIVGTNQLRIHRNQSALAIAVELLTS
                     HTPGAPVVDDAGQYIGFISEFDLLKALRSNQDLNQLTAEQVMVKDRITVTAETSIDDA
                     VRMMEEKRLLNLPVEKDGTIRYTLTRHDLLRAWIGLGLDIENQTV"
     gene            complement(13999..14217)
                     /locus_tag="PK224_12425"
     CDS             complement(13999..14217)
                     /locus_tag="PK224_12425"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRB16590.1"
                     /translation="MACNVGGIERPVRIVLGVLLIGIGVFAGLPPVGMGVALAMGAIA
                     LVTGAIGFCPVWTLLGVNTCPIDAGKKS"
     gene            complement(14415..14885)
                     /locus_tag="PK224_12430"
     CDS             complement(14415..14885)
                     /locus_tag="PK224_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16591.1"
                     /translation="MNWRVLGLGTAVLWMVTVGVIVALFVQGHTRPGADGRTEILLAP
                     AERDLILTEMRQLLKSVHGVVTVLGSPDQNLKAAEAAARSAGMAMAADVNPAVMLKLP
                     LAFKQMGMSIHKDMDHLADGIAQGESSIQILNRLSSMTARCTACHDMYRFATAK"
     gene            complement(14907..15275)
                     /locus_tag="PK224_12435"
     CDS             complement(14907..15275)
                     /locus_tag="PK224_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB16592.1"
                     /translation="MMNARVTGPVLLSMLLIAAVGSGSYAAGGNVAKGKVLYTNLCIR
                     CHGVDGKGDAYMKFNPPVADLSSPAVQSKLDESLMKAIHDGRKDTAMGAWKFVLSEEE
                     IRDVTAYVRVLGSHANRTLP"
     gene            complement(15501..15935)
                     /locus_tag="PK224_12440"
     CDS             complement(15501..15935)
                     /locus_tag="PK224_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRB16593.1"
                     /translation="MDVRSKVYTYRTAVKWTEQKKGTITCEGKPDIQVATPPEFKGHE
                     GIWSPEDLYVASANICLMTTFLAVAERAGLAFTSYHCEAEGRLELVEGKFQVTVITLR
                     PQIALQSGGDAAKAKELIEKAEANCLISNSMKTRIALEPTIS"
     gene            complement(16106..16522)
                     /gene="rnk"
                     /locus_tag="PK224_12445"
     CDS             complement(16106..16522)
                     /gene="rnk"
                     /locus_tag="PK224_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HRB16594.1"
                     /translation="MTPRDIYITEFDLTRLRDVLKARVNAEVRDRDHLDSLENELDRA
                     HVVDPSAVPHDVVTMNSQVRIEDVDTGMENVYTLVFPSDAGIQEKKLSILAPIGTALL
                     GARAGETVDWPVPAGVRTVRIKEVLYQPEAAGDYHL"
     gene            complement(16519..17496)
                     /locus_tag="PK224_12450"
     CDS             complement(16519..17496)
                     /locus_tag="PK224_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB16595.1"
                     /translation="MTTSVTTRILFGTDFSDDAVRAQEYAMYLATAWDAKVDVLHVIE
                     TPAWLNAAAATVAVVEQARNDAAQRLEQVYDHMVRSGISATIRQVVGNPAEQIALAAR
                     DNSSDLLVLGMQGRTNLVYGLIGKTVERVVKDGPCPVLAVPGIRDEVARPMDARPHVP
                     VRNILVAVDFSSPSLNAAEYAIQLANSLGCKLTLMHVLEPLCYDLDCGLGLIEHETRK
                     RDHWNRQLTELKDLITSFGLPAEVDISGGFPSDAILASALRHHSDLIVMGTHGRRGLS
                     MERFGSVAEAVLRRATCPVLTVKQPKFASGHRRVVPHQMEAMETKGAEQ"
     gene            complement(17857..18876)
                     /locus_tag="PK224_12455"
     CDS             complement(17857..18876)
                     /locus_tag="PK224_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB16596.1"
                     /translation="MRESSDPGGRRWSIVLAGGEGVRMQPAIRRWLGRPVPKQYCRFI
                     GKRSMFQHTLDRMMQATSPARTVVVVGRGHQEAWGQIGERHPGMVLVQPRNVDTAPGI
                     FLPLTYIRARDPEGTVVIAPSDHFVFPEERFLREVLYAVRAAEVLTDRVVLLAARPEG
                     PEPEYGWIKPDRELACVGARPVRAVRTFLEKPEPALAQLAYASGALWNTFLMAGKVDR
                     FWSLGKKALPKMMALFERLGEAIDTPKEAEVLREIYERMPRLNFSRDLLAQVPGETAV
                     MEMKGLWWSDWGSPERIVDTLTRLGKSDAPVVEHLKEAAAGYRPKGMVDIVLARDEED
                     EAQVV"
     gene            complement(18942..19073)
                     /locus_tag="PK224_12460"
     CDS             complement(18942..19073)
                     /locus_tag="PK224_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16597.1"
                     /translation="MGMLLALGVITCLVWGAILWLSFFDEKSVGLIGMPVKRESDIV"
     gene            complement(19623..20162)
                     /locus_tag="PK224_12465"
     CDS             complement(19623..20162)
                     /locus_tag="PK224_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16598.1"
                     /translation="MALDQDVLRSDYAHLLTLWTSGVRDYHTMLSDYLTANSIFVAVI
                     GLLVSRESLALPFTLIIVLFSIIGILMSVQMAIVLGRFSGQNALWEWQLRGIETMPEW
                     RERKPVSSLYRLREHRETIVEESNEPRSFEPSWAFRQHRQWWAHRAISFPWFFGTVYG
                     LFLLWGLTQVVRASTLSWG"
     gene            complement(20167..21888)
                     /gene="glgP"
                     /locus_tag="PK224_12470"
     CDS             complement(20167..21888)
                     /gene="glgP"
                     /locus_tag="PK224_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRB16599.1"
                     /translation="MTRQNSQTLVAYFSMEIGLNPLMPTYSGGLGVLAGDTIRSAADL
                     GVPMVGVTLLHRRGYFYQRLDQKGWQSEEPVAWVVNDFAEPMEPRVSVEIEGRTVHVR
                     AWRHHVTGMSGHTVPVYLLDTDLPENSPWDRTITDTLYGGDSHYRLCQEMILGFGGFA
                     LLRALGHGNIFRYHLNEGHAALLVLALIEAAIGTEPRNGDVPPEVIEQVREQCVFTTH
                     TPVPAGHDQFPVELAHRVLGSRRCDLVQACQHQGALNMTLLALRGSRFVNGVAMRHGE
                     VSHSLYPGYPIHSITNGVHAVTWTAPSFQSLYDRHLPDWRYDQLSLRYAISIPGAEIW
                     RAHQDAKLALVDEVNRQTNAGFDRDVLTIGFARRATAYKRGTLVFHDAERLRRLLKEA
                     GPIQFVFAGKAHPQDQAGKDMIHRIHGMRGQLSVAYLANYDMTLARLLCAGVDVWLNT
                     PLPPMEASGTSGMKAAMNGVPSLSVLDGWWVEGHIEGVTGWSIGEKIEACVSPAEDME
                     ACHARALYDKLEQRVMPCFYKDREGFIEIMKHAIAVNGAFFNTQRMVGQYLRNAYRLV
                     GQDVNGA"
     gene            complement(21885..23159)
                     /locus_tag="PK224_12475"
     CDS             complement(21885..23159)
                     /locus_tag="PK224_12475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16600.1"
                     /translation="MELKTQLPKVGIGRRCGSIQANLDGYRKLMSEEAYEELRTLAKE
                     LQGVKVCHVNSTAAGGGVAELLARYVPMLQALGVQADWRLIHGEADFFAITKAFHNAL
                     QGGHYGLGKAAQDLYLKVNERSAKALGNDYDVYIVHDPQPAAIRHFAGPRDAKWIWRC
                     HIDSSAPDKEVSQFLRPLLEEYEAVVFTMSQFVLPQLAAKWVAFIAPAIDPLATKNME
                     LPEDLCRRAIADSGVDPTRPLLLQVSRFDPWKDPLGVVRAYRLVKEDIPAVQLVLIGA
                     MAGDDPEGWEILDRVEEQAANDPDLFVFTNLAGVGSMEVNAFQRGCDVVIQKSLREGF
                     GLVVSEALWKEKPVVAGNTGGIPMQFPEPFQKNLVDSVEACAERLLHLLQRPGERGEF
                     GRAGREHVRRHFLMPRLVRDELRLIKQVLEAS"
     gene            complement(23212..23688)
                     /locus_tag="PK224_12480"
     CDS             complement(23212..23688)
                     /locus_tag="PK224_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16601.1"
                     /translation="MRTALKIGFSFGLTSGVITTLGLMVGLHAGTHSKSVVLGGLLTI
                     AVADAMSDALGIHIAEESKNHGNTNEIWESTLATFAAKFVIAATFAVPVLFMPLDLAV
                     AASLVWGLSLLAVLSYALARAQRVPPWKVIGEHVVIGLCVVAITHYLGDWVRVTFG"
     gene            complement(23953..24603)
                     /locus_tag="PK224_12485"
     CDS             complement(23953..24603)
                     /locus_tag="PK224_12485"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HRB16602.1"
                     /translation="MGVNRRVMKWVGVLLWSCALTACVVPRSGDNPVHTFVLAMDESL
                     SGANPRTGPAGTHGVLVVGVPQAEAGFEQPRMAYLQRPYEVNYYATNVWVDTPPRMLA
                     PLLLQSLERMSFWRVVVPMPTAARGDYRLDISGLVVQQEFLQRPSGTRIRLRAQLTET
                     KEQRIMGARSLESFEPAPSEDAYGGVLAANRAVTRVLESLNDWISSCLRGAGKDAC"
     gene            complement(24611..25573)
                     /locus_tag="PK224_12490"
     CDS             complement(24611..25573)
                     /locus_tag="PK224_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRB16603.1"
                     /translation="MEPKVNYILVGAFVVLLGATILGAIFWLGKTDYRGIYDRYYVYT
                     RESVAGLSVDSTVKYRGVDVGRVKQVILNPENAEEVRVTLDIVVGTPVKTDTQAVLVT
                     QGLTGLVTLNLTGGSREAPLLTPIAGQDYPVIKSVPSLFGRLDGTLAKLLSDQGLSTL
                     VANLNGLAQNASSALDEDNRTALKQILKDLSGVTKVLAERSGQMDRGVQQAVQAADQA
                     ARVTEQLGRQLPALVERLSRSAAGLQQMTEDLSRTSRSVGEMVGASRPGIEQFSRQTL
                     ADTGLLVTELRQLTATLNRVAQQVERQPNVLVLGRSAQTKGPGE"
     gene            complement(25584..26393)
                     /locus_tag="PK224_12495"
     CDS             complement(25584..26393)
                     /locus_tag="PK224_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB16604.1"
                     /translation="MTSTAVRIETPVIEVSHVSTRFGTAVVHRDVSLTVVRGEVFAIA
                     GGNGCGKSTLLREIIGLLAPTTGTIRLLGTDSRQLTHSNGSPLHRRFGVMFQHGALFS
                     SMTLAENVAVPLREHTPLSAEAIRDIVALKIALVGLPPDSAIKFPSELSGGMRRRAAL
                     ARAIVMDPELLFLDEPTAGLDPMIAAGFDDLVLELKRLLGLTVVMVTHDLDSLWRIAD
                     RVAVLGEGQVLGLGTMDALARSDDPVIRSYFHGPRGRAAHLNHSGTGLHRD"
     gene            complement(26490..27611)
                     /locus_tag="PK224_12500"
     CDS             complement(26490..27611)
                     /locus_tag="PK224_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HRB16605.1"
                     /translation="MANQQQPAGVTWLEGRTLRCRGAWTMPDVAPVERALRGMVWPAR
                     SVLVFEASGIEAFDTGGAVVLYRALMEARRQGCEVSIEGLRPDYAQLMNLVSSNWGAI
                     VAEVASRSNGTERFRTSIRNRVAQGARALEFLGESATALVRLVASPGRMRWRSCLNSL
                     RLDGVNALPITGLLTFLIGVVIAYQGAEQLRKFGTNIFIVDLVGISLLREIAPLIAAI
                     LIAGRSGSAYAAQIGTMKVTEELDALQTFGLSPIELLVLPRVLALVVALPLLTAYADV
                     LGVFGGMLIASNQLNVSFTAFLSRFEEAVALRHMLIGLGKAPFFAVIIALVGCYQGFQ
                     IRGGVDDVGRHTTISVVQSIFLVIIFDAICSILLNWWDL"
     gene            complement(27650..28804)
                     /locus_tag="PK224_12505"
     CDS             complement(27650..28804)
                     /locus_tag="PK224_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRB16606.1"
                     /translation="MTTRMVMLLILCMLPATAQADEPLSRAPWKERAVLRAGQIVQGD
                     YFAFGPHVEISGIVNGDLYAAGGEVLIDGVVNGDVIVAGAKVILSGTVAQDARIAGAQ
                     VTVSGTIGRNATLGGADVHLTETAKVRDNLIAGGGHVQLAGSVGRDARVGAWKATLSN
                     QIERDLIVAAGSVRLTSKATVGGRLRYWGEAAPSIDEEATVRGAITQRPLPEGWSIER
                     ARQGLVGIRLMAAFIGFVSTLILGLVLLRVYPVFARRVTATIRERPTAALGWGTVALV
                     ATPVVALSFVVTLFALPIGLLLFALYGATVYLARVYAITYVGQIVIRRQEDSSSLAWP
                     FVAGLVLYSVLSLIPVVGGLLTLLTVLFGLGALLVSKKELIETLREQKQV"
     gene            complement(28855..29280)
                     /locus_tag="PK224_12510"
     CDS             complement(28855..29280)
                     /locus_tag="PK224_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16607.1"
                     /translation="MHPIHPMVVHFPIALLLASTLFDALAFRWRSQQFRETSLSLLIL
                     GILAAGAAVLTGHFAEEAAERSGIPKQAIEIHEELGGSVFWIFLGLLGLRLASFWGWI
                     REQPRLVLIIGLGGGLLLLIASYFGGDLVYRFGAGVLPR"
     gene            complement(29611..31305)
                     /locus_tag="PK224_12515"
     CDS             complement(29611..31305)
                     /locus_tag="PK224_12515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRB16608.1"
                     /translation="MLVEQVMNQDGTDERPQSNAAIRRYVEIALLALVGGWAYFANLH
                     LSLLEGSEGLYAGIAGEMGRRREFFDLTYHDVPYFNKPPLFFWLLNLSTSIWGDHEVA
                     LRLPGSLAAVGTIVLTYVLGARLVSPTAGFWAALVVATSHVFLWYGRRVLFDSTLTFL
                     VTLALFAWIQVQLLGRNSRWYLLAFVSMALGAMLKEMHGFFLPLLVMALYAAIQRDTR
                     MLKDRYFWAGLAVAVAMMGAYAQMLGPGYQHHFRIGKAIESTWNTGFIGKAGPAIDGH
                     PLYWYLGMMWADFFPWCAVLPSALLLLWAQRPFRAHPTELLLLVWVLGLFTAFSLATL
                     KREPYLTTIVPGIGLMIGYYYHRLFSSAEDSAAMAPLLRLLLGVLAVTFAAGMFFGAA
                     PLRRRWLVSTAVVSPMFIVTIVSLAGLLLYAVLRSRVRLALGMVGALAIAYVVLVVNY
                     VFPAIDQAASPRKAVEEIKSLALGTQPALFMYIPGWPKNEDALYYLKRDNVVPDLPSQ
                     DAVLEAVRTHGAIRVVTEEQHAAALQAQAGLVVGTVQQFRQPGRKHLLLLSVQAQT"
     gene            complement(31567..31908)
                     /locus_tag="PK224_12520"
     CDS             complement(31567..31908)
                     /locus_tag="PK224_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB16609.1"
                     /translation="MMEIVNAREADLTQNPHQVKASRLYDTEHAQVVHITLEPGESLK
                     KHVTPVDVFFYVLEGRGIVEIGEDTKEVGPDMLIPSPARIPHRWSNRSDKPFRVLVVK
                     VPRPTEATKLL"
     gene            complement(31983..32903)
                     /locus_tag="PK224_12525"
     CDS             complement(31983..32903)
                     /locus_tag="PK224_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRB16610.1"
                     /translation="MRYDAILAARERYSGVGLTAVLLGTGLFLLGAGTVDRTAAEVEG
                     PPQPTPQWRVSTTVNYSSGSYGADSRTNILYAPMTVRRVFRDGDLSLTIPFVSISGTG
                     AVRLVGGMPTRTGSAAVSPTAALAGGAGRGKGPGAAPLSSATTDSGLGDVILRGRYYL
                     IEESAVMPLVAVTGRVKLPTADADLGLGTGEFDEGAGLELTKSLGDRWLAYLDGGYNL
                     IGDAPGTNFNNQWWYDVGVGYDVTDNLHMSLFYEEYRALVNTVNNARDLLALTNYIVN
                     DTVHLTGSLLVGLSNGAPNYGFGGGIRFRF"
     gene            complement(32921..33205)
                     /locus_tag="PK224_12530"
     CDS             complement(32921..33205)
                     /locus_tag="PK224_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16611.1"
                     /translation="MKLRKMLSIAVAVALSFPAGNVIIASAQDRDRDQLKTQQQIQDK
                     DKLQTKDQLKEKEQLKEQERTRERTREQTRTERPERPQHEKAERTERGGR"
     gene            complement(33374..33850)
                     /locus_tag="PK224_12535"
     CDS             complement(33374..33850)
                     /locus_tag="PK224_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16612.1"
                     /translation="MGSKGHTLCRVMVGLWIVTLAVAGWMFVAGWTKPGTDGRQEIGL
                     APAERDHILAEMRQLFKAVDGVVRGLGEPQPDPKAMEAAARAAGMKAAADTEPAIMAK
                     LPLPFKQMGLSIHKDMDALADAIVQKETPQQILQRLSSMTARCATCHDLYRFGVNQ"
     gene            complement(34129..35079)
                     /locus_tag="PK224_12540"
     CDS             complement(34129..35079)
                     /locus_tag="PK224_12540"
                     /inference="COORDINATES: protein motif:HMM:NF018439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HRB16613.1"
                     /translation="MDMFWKAMIGVIGLTAMTVGEVRAEEAPDMKNGEVIDCRYEQSD
                     SGTSSSAFPGDDVFRPLMADPKQPQFFASYQSVQRREPTSTVKGVGKSVNVGSVGFGE
                     NFGFYTKRQGCNGSQVGLLAGVFSQFNLDAPSSDLINADYIVGIPLSWRHGAWSTRVR
                     LYHQSSHVGDEFLLENPGFNRVNLSFEEVEGIVSYEYRWVRMYAGGGYLIHREPAHLD
                     RNRVQWGMELRGPTVHSPFLGSMMPGLQLTPVLGADFKTFEELRWIVNHNVIGGFEWS
                     RPGAKRRFRFLVNYYRGFYPYGQFFSEKVESIGFGFYLAF"
     gene            complement(35086..36216)
                     /locus_tag="PK224_12545"
     CDS             complement(35086..36216)
                     /locus_tag="PK224_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB16614.1"
                     /translation="MFERITQMLIKEFIQILRDPRMRTVIFVMPLVQTLVFGYAVTTD
                     VTHVPTAIFDLDNSRASRELVARFTGSGYFDVVGYVDREEEARNLVDHGLAKAVLRMN
                     KGFGEDLRAGRTAALQMIVDGTDSNTAGIVLNYAGQIAGRFSEVVLHTRFVRATGQPA
                     APPRVALETRAWFNENLESRNFYVPGVIVLIVTLVTLMLSSMAVVREKELGTIEQIMV
                     TPIRQSEFILGKMLPFALIGFADVVLVTAIAGYWFDVPIRGSLMLLLGATSLYLMSTL
                     GIGLLISTISRTQQQAMMSAFFYYFPAMLLSGFVFPIANMPEAVQWLTYLNPLRYFLV
                     IIRGIFLKGVGPDILWPQMAALLMLGATTLWVATRRFQKTIG"
     gene            complement(36224..37357)
                     /locus_tag="PK224_12550"
     CDS             complement(36224..37357)
                     /locus_tag="PK224_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB16615.1"
                     /translation="MRPRRIWAVARKEGIHIIRDWRSLVMGIAIPILLLILFGYALTL
                     DVDEVPLVVWDQSGTQTSRNFMSRFEGSPYFALRRIVQTYAEVERAIDAGEALVALVI
                     PVDFAMDVGSGRTARAQAIVDGSDSNTATIVLGYLETVARTYNQDLAIEQAARSSGRT
                     VTVPLDLRPRVWFNADMESKNYIIPGLIAVIMMVIAAMLTSLTVAREWETGTMEQIIS
                     TPLKGPELILGKLLPYFGIGMLDVLIAVLMGEFVFEVPLRGNVALLFSMAAIFLAGAL
                     SLGMLISIVTKSQLLASQLAMVVTFLPAFLLSGFMYDISNMPNAIQAITHVVPARYFV
                     SLLKGIYLKGIGLELLMLEAALLAGFGLVVLLAANLLFKKKMA"
     gene            complement(37354..38328)
                     /locus_tag="PK224_12555"
     CDS             complement(37354..38328)
                     /locus_tag="PK224_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB16616.1"
                     /translation="MPEREPYAVVVQDLEKRFGSFLAVNRISLQVKTGEIFGFLGPNG
                     AGKSTTIRMLCGLLTPTAGTGTVDGFDIMREAERIKTHIGYMSQKFSLYEDLTVEENI
                     DFYSGIYRIPKAKKAERKEWVIGMAGLAQHRGSRTAVLSGGWKQRLALGCAILHEPPI
                     IFLDEPTSGVDPISRRSFWDLIYELAGQGVTVFVTTHYMEEAEYCDRLGLIYRGELIA
                     SGTPDELKTRFMQDDIIEVLCEQPQEAILVLGTVEGVKEAALFGKGLHVVVERQAKGV
                     IPAITNRLAERNFAVSRVERIVPSLEDVFVSLIEARDRQERPQAEVKG"
     gene            complement(38315..39271)
                     /locus_tag="PK224_12560"
     CDS             complement(38315..39271)
                     /locus_tag="PK224_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB16617.1"
                     /translation="MEAIKLVHLTRSFGTVRAVEDLNFTVEPGEIFGLVGPDGAGKTT
                     TMRLLTGVMEPNGGEAWVMGKHVVREAEEVKEDIGYMSQRFGLYPDLTVEENLQFYAD
                     LYGVPTKGLEHKIHELLSFSNLTPFKKRQAGRLSGGMKQKLGLACALIHTPKVLFLDE
                     PTNGVDPVSRRDFWRILYSLLKEGVTIFVSTAYLDEAERCHRVALLHQGRLLACDTPD
                     RVKTLMRGTILEVRVGRAREAAALLKRRVKVESVGLFGDRIHVVTMDRVQTAQEVSAV
                     LQAEGFTLHGLRPIEPSLEDVFVSVLTSEEAVPPQEPIDARA"
     gene            complement(39258..40382)
                     /locus_tag="PK224_12565"
     CDS             complement(39258..40382)
                     /locus_tag="PK224_12565"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB16618.1"
                     /translation="MGGDRITIPPRTGDSGPIGHGASPPVAAAPGAGRTIPTAWMRAL
                     LILSLLVLAVAAGLYFQSRPVPDSGVLRVSGNIEITDAEVSFKIAGRVVERSASEGES
                     VKAGQSVARLDTSELAQEVALRSAEVRAAEASLAELVTGSRPEEIAQSEAVVRRMQAD
                     VARARADFKRLKKLYEQDNVSGQDYDAAKSAVEVTTAKLGEAQEQLRLVQKGPRIEKV
                     ERARAQLQQAKEALALAETRLGYATLTSPLTGVVLSHNIEPGEFVAPGTPIVTVGDLA
                     HVWLRAYIDETDLGRVKVGQVVKVTADTYPDKVYEGRVSFIASQAEFTPKSVQTKKER
                     VKLVYRTKITVDNPHMELKAGMPADARIVLDGAEASGGSH"
     gene            complement(40454..41794)
                     /locus_tag="PK224_12570"
     CDS             complement(40454..41794)
                     /locus_tag="PK224_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB16619.1"
                     /translation="MMKRTLGIAILATFWMMSLVRAESWLPPPELRLSLHDAIQAAID
                     NNANVRLLKERIVAAQSAASTSLGALLPNVSGFMSGSNQTVNLAAFGLPPDRLSGLGL
                     QRPVTEPFDVYNARANLVQNVFSLSLIQRWRAAKTGVDVAGLEAEVTKRDVMATAGLL
                     YMEASRAEAAVKARLADLELSQQLLKLAQDRKQAGVATGLDVTREEVQVENTRQRLLV
                     AQNDHESAKLNLIRTLGIDFNVTVTLTDELTMAKVESQTPETALSVARENRAELKAQL
                     TRQKLASLSLSSVTSERIPSLSLNGDYGWIGLKPDEALATRSVGLMLSVPIFDGGQRE
                     GRISESRSRVRQELIRMKDVSDQVTLEVRNALLTLDSSTQQVTVAEKGLSLSMKELTF
                     ARDRFAAGLATNIEITNAQTSVARARDNLIEALFRFNASRINLARAKGEIEQLF"
     gene            complement(41791..42468)
                     /locus_tag="PK224_12575"
     CDS             complement(41791..42468)
                     /locus_tag="PK224_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRB16620.1"
                     /translation="MAHPKHLPAEQRRNDTVRALIALAAEQNPTDITTAAVAKRMGLT
                     QGALFRHFPSKDAILEAVMEWVAERLLARIDKAAQTASSPLTAVEAIFLTHIEFVTEH
                     PGVPRMLIGELQRSEDTVAKGMVRTLLRCYTERLIRLMEEGKKQGQLDASLDSAAAAT
                     LFVGTIQGLVMQSLLTGNVSQIRRQAPGAFAIYRRGIASGKLPTRESGVGHDHRHPVE
                     RKKWSDR"
     gene            complement(42658..>44087)
                     /locus_tag="PK224_12580"
     CDS             complement(42658..>44087)
                     /locus_tag="PK224_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB16621.1"
                     /translation="QAYVGTASPFNFNGLVRHYYLRDQPYEADIQINLVAKEQRKTQS
                     HDIAQRIRSAVQDIGRPFGANVKVLEVPPGPPVQSVLVAEIYGPDYERQRAIAREVRG
                     LFETTAGVVDVDDYLEADQVKYVFSVDRAKAALAGIPSEEIVRTLRLALEGADVGLVH
                     IPKENRPVQVRLRLPVIERTGLEHLGEISLRTTAGHMVPLSELIRVEQTVRDTAIYHK
                     NQKPVVYVIADVGGVGPDKGESPVYGVMGIGKKLEQLVLPEGYAMTQQYAVQPWSEQR
                     FAMKWDGEWHITYETFRDMGIAFAVALLLIYLLIVAQFQSFLTPVVIMAPIPLTLIGI
                     LPGHWLTGSYFTATSMIGFIALAGIIVRNSILLVDFIQHQEAEGVSLLEAVIRAGAIR
                     TRPILLTAAALMVGAFVIILDPIFQGLAVSLLFGVGASTVLTLVVIPVLYYLWMGGPR
                     NSPAGLLPKQEHTELPRPVAGSQPE"
BASE COUNT         9036 a        14625 c        11919 g         8507 t
ORIGIN      
        1 ggtggtcgat gacgcgggac agtacatcgg gttcatcagt gaattcgatc tattgcaagc
       61 cctggctgga tgtggcggtg aagacccggg cccccgtggc ctctgccgca cccgccgccg
      121 tcatcatcga atgctcagag tccatcgtga cgaaccgcgc cgccagttca ccgccctcac
      181 accatttggc cagcgattcc acaatttccg tttgcggcgt gatgggaaac gcaggaatgt
      241 aatccacgtc ggccagcctc gccccccagg ccgccgccag gttgccggtc atcatttggc
      301 tgtccatgcc accccgcctt cctgttcccg ctccgcaacg agggcatgcg tcggacactc
      361 gtgcacacag atctgacagc ctttgcaatg ggcatagtcg atcaccggcc ggtcatcctt
      421 gttcatcgaa atcaccccgt ctgggcaata gacgaaacag agccagcacc cgttgcactt
      481 gtcggcgacc agcacgggcc ggaatgttcg ccacccgccg gtttcgcgca gcacggaatt
      541 cccggtggcg ctgatcctgg ccgtgcccct acttggcgat tcataaaccg gggtgtggaa
      601 cgtcgtcgcg atagtcgccg gctctgtcgc agactcacgc gccatcgctt gtaccgaccc
      661 ataacacgtg agtgccacgg tttgattccc ctcaatgatc ggcggggcta gcccgagatc
      721 ttccaactcc cgcgcaatgg cctgccggag cggctcctgt tgcaagccgg acaaccgggc
      781 agcgacggca ccgagcaacg cgctgatcgc ctcccgctga ccgagatgct gcagggcaat
      841 ctccgtcacc cccaacgtcg tgatgcgacc gggcaacccg ctctgcgtgc gaatctgctc
      901 tgcctgcagc ggcgaattga cgaacaggat ggtctggtcg gtgatgccgt ccgccacacg
      961 ggctgcagga tcttccagca gcgatgcatc ggccaccacc accagatcgg gatgggcaat
     1021 gacccctcgt tcgcggatcg gctcccggcc gaaccgcgtg aatgccgcga ccggcgcccc
     1081 gcggcgctcg gctccataga tcggggaatc ctgagcctgg tacccgctca gaaacgccgc
     1141 cgtgccgaga atccggctcg ccgtcttggc gccctgcccc ccgcgcccgt gaaatcgcac
     1201 agcatacatt tcaggcccga tccgtctttt ctttcttgaa cgcttccttt cccgccgcca
     1261 gagccgcggc aatcgtcgcc tcggtctctt tcagataccg cttggcctca tcaacggtgt
     1321 gggagaggtc agcacggatc tctttggcct tttccaagac ctcgtcctcc gtccgacgcg
     1381 catagccttt gatcgcccga cgggtttcct cgccggaact gggcgcatac agaatcgccg
     1441 ccatcgcacc cagcatcgcc ccgctcaaaa acgcgatcga cacccccagt cccgaacagc
     1501 tgtcatgatg gtccgccatg atgatgtctc ctccctgctc tattgattgc tccggcttca
     1561 gcctatcccc tcgatacccc tcttgggaga aagcagagga cgtgccaggc aaggatgccg
     1621 gcgcagcaac gaaatacccg ctttggcaag ggttttcagg gagcaacgaa ccgcaacaac
     1681 ggcggcggag ttcgattggg gaattccaga accggcccgg actcggcact ggcgcaaaac
     1741 gcgccagcga tcaggatctc tccgaatccg gagggctgaa gaatgcactg gggaaaagga
     1801 acagggacac gaacgaagcg cggacgcaag aagggaccgg tcagatcttg ctgcgaaccc
     1861 accgcacaat atcttgaagc cccagcgccc ccggctgacg cgccaattcg cgccctcctc
     1921 gcaacagcac cagcgtgggg atgctggaga tgccgaacct cgcggccagg gactgttcct
     1981 cttccgtatt tactttcgcc aggcgaacct gaggttccag catctgcgca gcctgttcgt
     2041 aggccggggc catcatgcgg cagggaccgc accagggggc ccagaaatcc accaccaggg
     2101 gaatctccgc tcgctgcgca tgcagatcga aatccgccgc cgtcaaagcg atcgggtgac
     2161 cggtaaacaa gggcgcatgg cactgaccgc aattgggctg ctccgccaga cgaaccgccg
     2221 gaacgcgatt gatactccgg caatgcggac agaccagatg gagcatctcc gtcaccgctt
     2281 cacctggtcc atgcgggatc cagcgccatt accgatcgca atgttctcct tcacagcccc
     2341 cgcttccggc ccctcgatgc cccatgccgc ccatcccttc tcgcgccccc ctgtgtccca
     2401 tccctccagg aggccccgtt cctcgcccgt gaccatggcc ctcagcgaag gtgcgttcat
     2461 actggatcac ggcccacatt tcttcgtccg tcagctgccc cgcaaatccg accatgctgg
     2521 ttccgggcga cccgttctta atcacccaga aaatttcgcc ctccgtgcga tggcgccaaa
     2581 atccagggtg ctgaaaatta cggggggatg gattcagctg cgacgccacc ggcccatcgc
     2641 ccgccccctc cggtccgtga caattggcgc acgtcccttt cccgtgatag agggccttgc
     2701 ccttttcgac cacatcaggc gaatcaggca atggactctt gagcgcacgc gcctcctcga
     2761 ggtgctcgat cgggacgcgg ggcggctgca tatgttgggc gactgcaggg acaaccgtca
     2821 ccacggccgc cgccatgatc gtcaatatga tactgagcag cctctgcatc agaaatccaa
     2881 ttccaccatc ttgcgatgga accgagtgat gacgttgggg tccggtgtca ggcggatctg
     2941 cgcctcatcc ttccccttgt acgccaggag gttgagcaca tggcgcaaac tttccaatcg
     3001 cgcggcctgc ttgtcgttcg ccttgacgat gatccagggg ctgaaggtcg tatgggtctt
     3061 gctgaacatt tcttctttgt atcgcgtgta cgaatcccac agctcctgcg ccttttcatc
     3121 gacggggctg agcttccact gcttgagggg attctgccgg cgcgcttcga atcgactggc
     3181 ctgctcctct ttggaaatcg agaaccagaa cttgatgatc gtcacgccgt cctcgtagag
     3241 catgtgctcg aattccgtca cctgctgcaa gaaccgctga tgttccttct tgctgcaaaa
     3301 tcccatcacc ggctcgacga ccgcacggtt gtaccaactg cggtcgaaaa agacgatctc
     3361 ccccttgttg ggcagttgcc ggatatagcg ctggaaatac cactggcccc gctcatcgtc
     3421 cgtcggcttc ggtaacgcca cgacccgcat ggcacgcgga ttcaaatgct cggtaaaccg
     3481 gcggatcgtt ccgcccttcc cggccgcatc ccggccttcg acaagaatcg caatacgctg
     3541 cccatcggct tgtacccagc gttgaagacg gaccagctcg acctgcagct gctgcaattc
     3601 ctgttcatac agcagcgtct tgcgcacgtc atccagatcg atattcttgc tcttgatcaa
     3661 ctgcagcagc ccgcgccgcg tattcacgcg ccgcagatcg tcttcggtca gggttcggtc
     3721 cacttcaccg attaatccag ttaatccggc cgtcactccc ccggtggctt gatcaggagc
     3781 ttggtacccg tctccctccc tcggcacagc cgtcggaata atctccaggt catcagtctc
     3841 aagtcgtccg ttgtgttccg caatcgagac cgcggctcct gcagccatac gccccttttt
     3901 gcctttagct ttcctgcccg cttgttcgtc ggatgccatg cgaatacccg tccccttccc
     3961 acctatttag aactttagaa cgccacggtc cggtcgcgct cagccacagc cttgatgtag
     4021 tccggcatgc ctttgatcac ggctccggct atgagatcag cctggccgat ctgcctggcg
     4081 acggcacagg ctccgcagac ccagatggga atgttggcgg cctggacggc cgccaacagg
     4141 tctttcagcg gcgtcaaatt cacgccgacg acatgatccg ccgagccctt tcgcgctgac
     4201 gtcaccgcct cgttccacaa ccacaacacc acatcgtgac cctgctcctt ggcggtcttg
     4261 gccgccatga gcggaagagt cgccatggtc ggatcgtctg tgccccgact gccggaaatg
     4321 ataaacgtcg ccactaacaa cctccttcgc ttgccattcg tcgtccgtat ctcgtcgttc
     4381 gtccggaacg tttcacgaga tacgtttcac gcttcccgtt cttacttcgc caaacttctc
     4441 acataggcaa tcaactgcca gacctgctca tccggcaatc cggcgaacga catcatgccg
     4501 gttccagccg agccgttctt gatgatccag aacagttgcc cgtccggaag atctttcatc
     4561 attgagccgc acgtaaagtt tcggggaggc gggatcaagg ccgcgcccat gaagccttgc
     4621 ccgttgcctt tgtcgccatg gcacattgcg caggccacgg gctgggccgt ctggagaaac
     4681 agggttttcc ccgccttgat ggtcgcatcc gactgcggca acggattggc cttggataga
     4741 aactctgccg gcgccttcgc cgttttccga ggctgcgggc agaccccgct cggaccttca
     4801 ccggaccctg cggctgcctg tgcgacttcc gcttggccct tgaaggtcac tttcaaatag
     4861 gccatgacat ccgcgacacc ctgttgaccg atggtcagac cccaataagg catgctcggc
     4921 gattttccca tcgccgcccc gccgtgattg atcacattgt aaagatagtc ggtcggcacc
     4981 ttgtcgaacg ggatgttcgc atggatcgcc ggcttcggtt ccaatccgga cgcagccgga
     5041 ccgtcgccct ttccggtgaa cccatggcac tgagcgcaat attctttata gaggaccttc
     5101 ccgcgatcgg cactcgccat cccgaactcc ggcgccgtcc agggttcgta atacttgaag
     5161 tccttgacgc cctgcaccat caggtacccg atgacggcac ggagttgcgg ttccgtcgcg
     5221 tccgccagga actccccgtt gtgaaccgtg aaatcttgcg gattctgtcc gaaccggaag
     5281 agccaatcct tgtcgtaccg ttgaccggcc gctaccagcg acgcgctctg cggcccgccg
     5341 atcagcttgc cgttctcctc gaccaaatga cagccgagac aggcgtgagc cttgtaggcc
     5401 atgccgccga acgtcgcatc gaacttgctg accttcgaga ggtcgaaggc cccgactttc
     5461 acccgcgggt ctttattgtg ttgttcgaaa tactcggcaa tacccagggc ctcgtcttcg
     5521 ctgaccaccg gatgccgcgt cggcccctca gacaaatccc agcgatatcc ctttggatac
     5581 agcggcgcct ctttccccgt caggtaacga agcaaccacg agcgctgata tttgctcccg
     5641 gcccaaatca gatccggcgc cttcaaattg aaacgcgaat ccgctttccc ttccaagcgg
     5701 tgacaaccgg cgcaattgtt ctgaatcatc tccttgacgc gagtctggtc actgcctaac
     5761 agcaagcggg ggaatgagaa caaccccacc aacatgacca acgcgcatcc gactaccacc
     5821 gtccgtcttc cacccatgaa acaccccttt ctttcgcccc actgccgtac cggtcagcgt
     5881 gtcgccaacc aggcatgaat atccgcgatc tcctgatgac tcagcactgc gccccaggac
     5941 ggcatcggcc cccgcccatg gagcaccgtc tcccaaaaag ccccttcatt tttcagaatc
     6001 gtattgcgcg cgagtttcgg tccggcccca ccggacgctc cttcaccatg gcaggcctgg
     6061 caattgtgcg catagagtcc cgcgcctctc gatgccacac cggacaccgg cccctcagac
     6121 ccctgacgcg ggcctaccgc aggcccgatc atggccaact cttcttcaat cgacggcaga
     6181 tggtccggcg caccggaatg ataccgcgcc gtcacgaatt gcaagaccgc cgccgcctcg
     6241 tccttggaca gatccgcgcc ccaatgcatc atcttctcaa ccgtcactgt ccatcggtcg
     6301 gccggcaacc gttgctgcac gatcaggtcg gtcgtgtggc agacggcgca acgggcatga
     6361 atcaatgtca gtgcttgacg agcctgggcc ggctgaatcg ttccacggtc atcttcctga
     6421 gccgccgtga tcaccaccca gccaagcaga gtacagagca atgcagcgag cacgaaaccg
     6481 gaggtctttt tcgccgtgct ctgggtcatg ccgccaccgt gacggacagg cgatcccatc
     6541 cgttccatag aaaccctccg ggattccaag ggctcgtcgc cggttgctgc tccccctgcg
     6601 catcggtggc gcggcagaga atcgcagtag gcccgggcgt gcgggcgttc cacacaacct
     6661 gccattgtcg ccaggcatag ggctgcccct ctcccaataa ccgggcctgt tcccaggtct
     6721 ttccgtcgtc gcaggacact tcgaccttca ccaccggcgc ctcgccggcc caggcgacac
     6781 cttgaatcgt gaccggcccg atccgtaccg tgtctccctc cgccggagcg gcgatgagcg
     6841 acttgacggg catctgctcg accggaacca tcgcgcttcc cggcaatccc gaagataggc
     6901 gaacggccgt ttccggaatg cgataggcct gctgcatgta gtagccgggc gtttcatccg
     6961 ctcgaaccgt aatctcggtc agccacttca tgcaggactc cgccatccag ccgggagtaa
     7021 tcacccgcag cggcgcgcca tgcagcaggg gcagcggacg gccgttcatt tcataggcca
     7081 ggagggtatc gggatggaga gcctttgcga gggggatact tcgcgtgaac agcggcacgg
     7141 tcgccagcgc cggccggtcc gccccctgca attgcacgtg gactccctgc ggtttcactc
     7201 cggctcgctg caacacatcg cgaagccgca cgccggtcca ttgcgcattg ccgaccgctc
     7261 cacgttccca ctgcacaccg ggcactttgg gacgatggtg cgcgcgtccg tttccgctgc
     7321 attgcaggac ggcggtcagg gtgacggcct cgagttgttg taagtctttg aggttgagcg
     7381 tcagctcctc tttgaccagc cctttgaccg tcagtcgcca gacatcgggc tgtaccgtct
     7441 cggctgaagg agggccgaag tgactgcgcg tgaaaaatcg ctcattgggt gtgagccagg
     7501 aggcaaattc ccggaccggt gtttctgcgt cgaacggcct ggtcactcgt acggtcaacg
     7561 gcccggttcc ctgccccgcc tcactcccct gcgcggtggc gatcgaggtg cccccaggcc
     7621 cgaccaccat accgatgccg attccttgca gtaccgttcg caaccattcc cgtcgggaca
     7681 ccgtcatgac ttttcctcca taagatcaga tgccgtcgcc ggatggccgt gcggattcag
     7741 ctcgatcgcg atagcggcag gcacgacgat cgattgatgg accgtgcacc cgtgcgccac
     7801 cttcagcagc cgctctctca gctcgggtgt cactcgatgg ggaaggcgaa tcgcaagttg
     7861 gatctgcccg actcgatgcg gcgcttcagc tatcgtccat tcagcgtcca cctttaggcc
     7921 ttcgcgcgag atatcgtgtc gcgcgcagaa ctggccgaca aagtacccca cgcagctcgc
     7981 caccgaaccg acaaacagtt ccacgggact cataccggcg tcggcccctc cgtcttcaac
     8041 cggctgatcc gtcacgatcc ggtgccggtc gctcaggatg tcgtaccgcg ctcccccctg
     8101 atacgccacg ctgagtttca tttcgtcctc ctccgctcgc ctctcgtacg tcgcatctcg
     8161 ttgttccttc ccaagagggg tgtgttacga gatacgtgtc acgaagagcg tctcacgcgt
     8221 tcaatagggt gcatccgccg gtttccctcc cttcggccag tcattgcgct tgtcgggaaa
     8281 atccgggcgt ggtttggagt tgatatacgc cgctacgtca taggcatcct gatccgatag
     8341 ggcccatcct ctggtgcgag gcatgttcga tttgatgaag gaagcggcta ccggaatgcg
     8401 cgccatctcc gcgccgatgt tgtaagactg aggtccccac acagcgggag ctgccatcgt
     8461 gccctgcccg tccgctccgt ggcaaaacgc gcaacgggat gtgaagacct tggcaccatt
     8521 cgccacatcc ggcggcttgg gtgcttgaat ccgtgggatg ccgcgccagg ccatccggct
     8581 tcctgccgga acgtccttcg agagccattc gatgtagtcc acgatggccc ggagcttgtg
     8641 gctgtccgac ggaatcggct tgccgttcaa gctgcgttca aaacattcat tgatgcggtc
     8701 ggccaatgtc acgacacgcc cggttcgcgc gcgatattcc ggataggccc gcgagagccc
     8761 gacataggaa gccgaattgg gatcgagccc tgcgtccaaa tgacaattgg cgcaagtgag
     8821 ccgattccct acgtagggtg cggcgaattc ctgcgtatga acgacgagct cataccccag
     8881 ccggatctgc tccccgcgct ggccgccggg gatggaatcg ggggagggat ggctgaagag
     8941 ggcatcaagc ctgtccggct cggcttgact catggcagac tgtgcgccgg tgcgtgtttc
     9001 cttcgagtcg acgcaacctg ccatgaacgc gacgatcatg atggcgctga acaataggtg
     9061 tttcatacgt agatagtctc gcaaactaca taccacgtcg acagaagctc gcctacgaga
     9121 attagctttc gacttgccct aaggggcatg cgtatctgca tacttggcga gaaaagccac
     9181 agtccctccc tcccgcctgc ggcatcgtgc tacagcgaac tcatacacca ccttcttgaa
     9241 tgtggtgatt agggacaggt ttggggaaaa ggattcagaa tgttgatttg atcgttctga
     9301 ggcgttatct gccctgtttc ctcgcgccgg cccgggaggc ttcgaggatg gacgaaggga
     9361 ctaacgtgat caccggccga tataacgtcg gtgccttgca ctcatcattc acgcttccga
     9421 gcatcatgtc tttggtgttc tcgataatcc tacgccgcta cggggattcc ggttccggcc
     9481 acaacatagg cacggcccta cgcagcctat gccgtaagcc gtagccccct tcgcagcgct
     9541 cggatcacgt cgaacagctt gatccatcct gagacgagtc actccgagat cggaatcgag
     9601 atcaggaccg cgccgagcac gtcccgcatc ttgtagtccc gtttcgggct gcggggatcg
     9661 gcgttatggc agctcacgca cacggacgac acggcaagat ccgcatacag ggcctgaaag
     9721 gatcgcacgc ctccctcggt caccgacccc ttatacggct gctctggatg tgccatgacc
     9781 gcttcgagcg ccttgcgctc gaattcgcca tgaggcccgt tttgtttgtt gatcgggttc
     9841 aggctgatca agcgataccg gacggcgctg ccggtcagcg tggccaggct cgatgcctct
     9901 tgaaagaact gggctggaag cggaagggcg ctgagttcgc gccatttctc agaagactcg
     9961 atcacgcccc tccgctgcat ccgctccacg acatggacgg tataaaatgt gcgattggcc
    10021 tccaggacgg aatggagata gtccatcacc gcttcaaccg ggacactgcc ggccgatggt
    10081 gcgccggcaa agctggggcc ccagcctccg agcagagcaa ccgccacaac cagtcccccc
    10141 gcaagcttct gtgtgatcga cataacagcc tcccttcatg atggtcctcc ggccaagaga
    10201 cgaccaccgg gtacagtctt gtgatactct tcttcgaacc accagcaacg cctgcctgaa
    10261 gccgagccgc cacggacgcc acgttcaaac cagcagggga ggtcgaggtc cgctgttgcg
    10321 ccctcctcga acctcatgac ttgagcgagc ggatgtaggc gatcaaatcc gacaattgag
    10381 gttcttcaaa cgctccctca tacccggtca tggccgtacc gggccgcccc tccaggataa
    10441 ccttccgcag ttgcgcatcg gtctttgact gggtcccctt ggcggtaaga ttggcgggcg
    10501 ggggcgacag gtaggccgcc atgtcgctgt cgcccttccc cgtcggtcca tggcagttct
    10561 gacagctctc cagatagatt ttcttgccgg cctcaggatt cgcctttccc tcagctcctc
    10621 ccgcggctcc gcccatgaca atgttcgcaa ccatcaacag gagtcctccg cccgcgactg
    10681 tcgcccggcg acctgcatcg tccattgctt cctcctctca ctcaggctat ttgaatgtca
    10741 tcatgtacgc cgtgatggcg taaatgtcct catccggcag cggcggctta gggatatagg
    10801 ccccgctgtc gggctcgaaa tcgttcgggt tcaggttaaa acggtagacc caatccgcgt
    10861 tcaaccgctc gctcaatttg atgaacgagg tactggatgt tcctccgaca taccccttcg
    10921 gcgtgccggc cggcgtgtaa tgacaattct ggcatccatg ctcctgatag agtttgcgtc
    10981 ccctcgccac ctgctccgcc gtgcctgcct tcatcacacc ttccttgata cggggatcct
    11041 tccgcgtagc cagaaacgcc gccgccgctt tcgcctgatc gagggacaat gcaaggtgtc
    11101 gcttctttcg atcaggacga aaatcgtatc cgaccgggta gagcttgaaa tcgggattct
    11161 ggagccagtt gacgagccac gctggctgat acttattacc ggcccaaatc aggtccggtg
    11221 ccttcttggt tttgcgggct gcgaccggac cttcgattcg atggcagccc gtacaggtgc
    11281 tcctcacaac ttgttcgccc gcttgctcac cctttgccgc aacgcgagtg accgcaaccg
    11341 caaagaccgt cgtcatccca actacgagcg ccacaagaag gccgccacta cgccagacgt
    11401 cggtcatcat cgctcctttc cgttctctgc ccgcattaga cacaccctaa ctctcccgct
    11461 tcaaactcag catgtagctg gtcaagtcat tgagctgttg gtcggtaagc gggaatttcg
    11521 gcataaacga tcccggcgag acggattgcg gatcgcggaa atgcttgaga tgccacgccc
    11581 gatccggtct cgtgtctccg atcgtagaca ggtctggggc gacggcgcct ccctcaccat
    11641 gaatgcgatg acagccggca caaccgagct gggcatagat cgctcgtccc tggacgacgg
    11701 ccggatcggt tctcggcaca gcgtacagat tcttgagcga gatgcctaag agaccgaaga
    11761 cgacgaacag gaagagcagg ccgctgccca aggccaccgg ccgccggatg ggattgcgca
    11821 ccgggttacg atcgatgaac ggccagcaca acatgatcag aatcacagac agaggaagaa
    11881 cccaggtcgc caacggctca agcggcccat gcacgtactt gagcagctcg tagtaaaaca
    11941 gaaagtacca ttcggggacc ggcacgaagc tcgtatcgga aggattagcc ttatccgtca
    12001 ggggcagagg aatgctcaag gccagcgcgg cgaccacgag aaacaccagg gcaatcacca
    12061 ccgcatccat ataaacctgc cggggagcga aggtctcgct gcccagcgac gcgcgatcgt
    12121 cggtccaggg tccggccggc cctacccgcc tcaacacgaa cagatgcaga gcgatcccgc
    12181 cgatcaggat ggcggggaga aataacacat gaatcgcaaa gaaccgtgac agcgtcaatg
    12241 cgccgaggat ttctcctcct cgcaagaccc gggagagaaa atctccgatg agcggaaccg
    12301 tgcccaccat gttgatgccg atctgagtcg cccaataggc ggtttgatcc cagggcagca
    12361 aatagccggt aaacgcgaag gtcatcacca cgaggaacag caccaccccg accatccaca
    12421 tcacttcgcg cggctttttg tacgcgccat agagaaaggt ctggagcata tggagaccga
    12481 tcgccacgac catcgacgac gcgccccaat ggtggagccc gcgcacgaac cacccgaacg
    12541 tgacctcggt ttcaatgaat cggacgctgt cgtaggcgtg atccggcgtc ggagcataat
    12601 aaagcgcgag gaacatgccg gtggcggcct gaagtacgaa caagaacaac gtgacggagc
    12661 cgaagacgta aatccagctg gctccgcccg gaatgggttc gtcgagcagc gtgcgctgca
    12721 ccggtttcag gttcaaccga cggtcgaacc aatcgtatag gcgagaagac attcaactcc
    12781 cagatctcgg caatgaaacc atcgccgctc acaactccac actctgtgga aggcccgatt
    12841 taaagtcttg atacatgacg aggagccggc ctccctccac tttcgacggg agtacgtcca
    12901 gcggacgcgg cgcgggtccg cccaacacct gaccgttgat gtcataggcg ctcccgtgac
    12961 agggacagag gaacttcttt tccgtatcgt cccagcggta cccgcatccc agatgcgtgc
    13021 agatgggcga gaagaccttc acctcgcctt gcgcctgctt caccgcccat accgccttct
    13081 gggacttcgc ttccagccag ccatcgcgga cggtggtcac atgatccaat tgggtagggc
    13141 gccccgcggg caacgtatcc accgggccga catcgaccca ttcacgtttg cgtcgcatga
    13201 aggccggtga caccagcgag ccgagcagtg gcacagccag ccccaccccg accacagcgg
    13261 ccgcggcaac cgtcacccag tgaaaaaatg tccgccgcga cccgaccgga gtcgacagcc
    13321 ctgccccttc acagctctgt gtgtcgtcgg aataactcac gttcgtaatc catcatgtag
    13381 ggtgattccg ggcaggagag gaggcggcag cctattcact cccgccccct ccgccattcg
    13441 caacttgatt cagaccgtct ggttctcaat atccagcccc agaccgatcc aggcccgcag
    13501 caaatcgtgt cgggtcaatg tgtatctgat cgtgccgtcc ttctcgacgg gcagattgag
    13561 caaacgcttc tcttccatca ttcgaaccgc atcgtcgatc gatgtctccg ccgtcacggt
    13621 aatccgatcc ttgaccataa cctgttctgc cgtcagctga ttgagatcct ggttcgacct
    13681 cagggctttc aatagatcga attcactgat gaacccgatg tactgtcccg cgtcatcgac
    13741 caccggcgcc ccgggtgtat gactcgtcag caactccacc gcgatcgcca atgcgctttg
    13801 gttccggtga atgcgcaatt ggttcgtccc tacgatctgt ccaaccgtct taaatccgcc
    13861 taccggcaca cccggtctcg ccatcgtcgt catcctgtcc tccttcgcgc tactccagtg
    13921 acggaattgc caccggcact ctccacactg cccatgatca ccctgcgcac acgccacttc
    13981 accgcaaaac ctgttgggtc aggatttttt acctgcgtcg atcgggcagg tgttgacccc
    14041 caacagagtc cataccggac aaaacccgat ggcgccggtg accagcgcaa tcgctcccat
    14101 ggccagcgca acccccatcc ccacgggagg cagcccggcg aaaacaccga ttccgatcaa
    14161 caagactccc aacacgatcc ggaccggccg ttcgattcca ccaacattgc atgccatcac
    14221 agcacctcct tgcgtgcgat cacgatctca gtcgccagcc ttcgaccatg cgcgttcggg
    14281 ttcaaagact gtcggcgact cgctatctcg atgagtatga gtcaccgcaa aagaaatgga
    14341 ttcaggatgt actcgtccca ccggctctcg attctccgaa catcccctcc gatgcggcac
    14401 ctgacagccg accgctactt cgctgtcgca aaccgataca tgtcatggca ggcggtgcag
    14461 cgcgccgtca tgctcgacag acgattgaga atttgtatgg aggattcccc ctgcgcaatg
    14521 ccgtcagcca gatgatccat atccttgtgg atcgacatgc ccatttgttt aaacgccagg
    14581 ggtagtttca acatgacggc gggattgacg tctgccgcca ttgccatacc cgctgagcgg
    14641 gctgcggctt ctgcggcctt gaggttttgg tccggactcc ccaagaccgt cacgacccca
    14701 tgcaccgact taagcaattg ccgcatctct gtcaggatca gatcccgctc cgccggcgcc
    14761 aggagaattt ccgtgcgccc gtcggccccc ggcctggtat gcccctggac gaacaacgcc
    14821 acaatcacgc ccaccgtgac catccataac accgcggttc ccagcccaag tacacgccag
    14881 ttcatgaggc actcctttcc attcggctac ggcagcgtgc gattggcgtg actcccgagc
    14941 actcgtacgt aggcggtcac gtcacggatc tcttcttcgg agaggacgaa tttccaggct
    15001 cccatggccg tatccttccg tccgtcgtga atcgctttca taagggattc atctaacttg
    15061 ctctgcacag ccggagagct gagatcggcg accggaggat taaacttcat ataggcgtcg
    15121 cccttcccat cgactccatg gcaacggata cagagattgg tatagagcac cttgcctttg
    15181 gccacattcc ccccggccgc atacgaaccg ctgccgaccg ccgcaatgag gagcatcgac
    15241 aggagaaccg gaccggtgac tctggcgttc atcatcttct cctttgtaag gagctgccgc
    15301 acacccgtgg caccccacat acgacgatga gaatgcgatc ctcgtgccag tcggacagga
    15361 agggacaacc aagtgaaatg agagaaacac ggtgagagta accgattcgc ggaaaaactc
    15421 gacgcccagc cgggatggcc gtggagagaa tcaccccacc gctcgatccc ggctggcgca
    15481 agatgcccca atccggagca tcaggagatg gtgggctcta gggcgatacg cgttttcatc
    15541 gaattcgaga tcaggcagtt ggcttccgcc ttctcgatga gctccttggc tttcgccgca
    15601 tcgcctcccg actgaagcgc aatctgcgga cgcaacgtaa tcacggtgac ctgaaacttt
    15661 ccctctacga gttccaaccg tccttccgcc tcacagtggt acgacgtgaa ggccaatccg
    15721 gcccgttcgg cgaccgccaa aaatgtcgtc atcaggcaga tgttggccga cgccacatac
    15781 aagtcctccg gcgaccagat gccttcatga cctttgaact ccggtggtgt cgccacctgg
    15841 atatccggtt ttccttcgca ggtaatcgtt ccttttttct gttccgtcca tttcactgcc
    15901 gtacgatacg tatagacctt gctgcgaacg tccatggatc cctccgattt gacgatgacc
    15961 tgatccgttg cccgttgaat ggagagcggc cacttcaacg attcgacatg cccattttcc
    16021 tcccgtcacc ggatcatgag atcgacagat tacgccgtgc cggtctgata ataaccagca
    16081 ggacgcgcga aaagaccagc aagcgctaaa ggtgataatc ccctgcggct tcaggctgat
    16141 acaggacttc cttgatgcgc acggttcgca ccccggccgg cacgggccaa tccaccgtct
    16201 cacccgcgcg cgcgcccagc agggcggtcc cgatcggcgc aaggatcgac agcttctttt
    16261 cctggatacc ggcatcggaa ggaaatacca gcgtatagac attttccatg ccggtatcga
    16321 cgtcttcgat ccggacttgc gaattcatcg tgaccacatc gtgcggcacg gcggatgggt
    16381 ccacgacatg ggcgcgatcg agctcatttt ccaaactatc cagatggtct cggtcccgga
    16441 cttcggcgtt gacgcgcgcc ttcagcacgt ctctgaggcg ggtcaaatcg aattcggtaa
    16501 tataaatatc gcgaggggtc attgctctgc tcctttggtt tccatggctt ccatctggtg
    16561 cggtactacc cgacggtggc ccgatgcgaa tttcggctgc ttcacggtca agaccgggca
    16621 ggttgcccga cgcaacaccg cttccgccac gcttccgaat cgttccatcg atagaccgcg
    16681 gcggccatgg gtgcccatga cgatgagatc ggagtgatgc cgcaacgcag aagcgagaat
    16741 cgcatcggaa gggaaacctc ccgagatgtc gacttccgcc ggcagcccga aggaggtgat
    16801 gagatccttg agttcagtca gttgcctgtt ccaatggtct cgctttcttg tctcgtgttc
    16861 gatcagacca agaccgcaat ccaagtcgta acacagcggt tccaacacat gcatcaacgt
    16921 gagcttgcac ccaaggctgt tagccaattg aatggcgtat tcggccgcat tcaacgatgg
    16981 gctcgaaaaa tccaccgcca ccaggatatt ccggaccgga acatggggac gcgcatccat
    17041 cggccttgcc acctcgtcgc gtatcccggg cacggcaaga accggacagg gcccatcttt
    17101 cacgactcgt tctaccgtct tgccgatcaa cccatacact aaattggtgc gcccctgcat
    17161 acccagcacc agcagatccg aactgttgtc cctagctgcg agcgcgatct gttcggccgg
    17221 atttcccacc acctggcgga tcgtcgccga gatgccgctg cgcaccatgt ggtcgtacac
    17281 ctgctccaac cgctgcgccg cgtcgttccg cgcctgctct acgacggcga cggtggcggc
    17341 agcggcgttc aaccaggccg gcgtttcgat gacgtgcagc acatccacct tggcatccca
    17401 ggcggtggcc agatacatcg catattcttg cgcccgaacg gcatcatcag aaaagtcggt
    17461 cccgaacagg atcctagtcg tcactgaggt cgtcataccc tgctcctgta aagacggttg
    17521 ggcggctgct gccgtgcgtg cgaccggacc ctcgatcagt cgaagaccac cacgacgagc
    17581 caagagccgg agatcatttt ttgtatcgac gttgcgaaag cccggcggta atcagccgac
    17641 acgggatgga acgagcaaga cggatgccag cctctcaccg acccacgact gcaaggagtt
    17701 tggaccattc ccttgccttc gctccaaaga agaggagacc tccaccgacc gactgttgct
    17761 atatgcggca gaggcttctc tcactgtgag gcaatacacc cctcacccac gacatccgct
    17821 cttgtgatcg cgcggcactt gccgtgcgac ttcgatctat acgacctgcg cttcatcttc
    17881 ttcatcgcgc gccagaacga tgtccaccat tcctttcggc cggtagcctg ccgccgcttc
    17941 cttcagatgt tcgacgaccg gagcgtcact tttacccaac cgggtgagtg tgtcgacgat
    18001 ccgttcggga ctcccccagt cgctccacca caggcctttc atctccatca ccgctgtctc
    18061 gcccggcacc tgcgcgagga ggtcccgtga gaaattcaag cgcggcatgc gctcatagat
    18121 ctcgcgcaac acttcggctt ccttcggcgt gtcgatcgcc tcgccgagac gttcgaagag
    18181 ggccatcatc ttcggcaacg ccttcttgcc caggctccag aaccgatcca ccttccctgc
    18241 catcaggaac gtattccaca aggcccctga agcataggcc aactgcgcca aggccggctc
    18301 cggtttctct agaaatgtcc tgacggcccg caccgggcgg gcgccgacac aggccaactc
    18361 acgatccggc ttgatccacc cgtattccgg ttccggccct tccggccgtg ccgcaagcag
    18421 taccaccctg tctgtcaata cctccgccgc ccgaaccgca tacagcactt ccctgagaaa
    18481 ccgttcctcg ggaaacacaa aatgatcgga gggagcgatc acgaccgtgc cctccggatc
    18541 acgcgcccgg atgtaggtca agggaagaaa aatccccggc gccgtgtcca cgttgcgcgg
    18601 ctgcaccaac accatcccgg gatgccgctc accgatctgt ccccaggcct cctggtgtcc
    18661 acgcccgacc accacgaccg tgcgtgcagg actcgtagcc tgcatcattc gatcgagggt
    18721 atgttgaaac atcgacctct tcccgatgaa ccggcaatat tgcttgggaa ccggccggcc
    18781 aagccagcgc ctgatcgccg gttgcatccg gacgccttct ccccctgcca acacgatcga
    18841 ccaacgccgt ccgcccggat ccgacgattc ccgcatgatg acctccttcc atcgaaagaa
    18901 aacggtttct gtggccatga ggcccttcag cagccggctt cctaaacgat gtcgctttca
    18961 cgcttcaccg gcatcccgat taatccgacc gacttttcat cgaagaacga caaccacagt
    19021 atcgcccccc acacgagaca tgtgatcact cccagcgcca acagcatacc catgttccac
    19081 ctcttcttcc cgatccgtct cagttgagcg tccgacttgc tattcggaga ggtgcaggag
    19141 cgacaagccc cagaaaacca tgacgcccat cgcggcgatg accaacaaca gcagcagcat
    19201 gaccggcacg atggtgttca gtttgttcat ctcgttcacc gttcacgatc tgtccgggac
    19261 cggatccccc tctctccgcc ctgccatggt gacaatgtac ccgctcacca tgaaccgacc
    19321 atgaacggag catgaagaat tccacatgaa tcgtttttca tgatggcttc atctccattt
    19381 catatccgcc tggtatgaag caacggaggg tgagctgctt gaaaggcgtg ataggacggg
    19441 cggcgacagt gtttgttgcc gacgccgctc tcaattcggc gcggctggtg ttcacggcga
    19501 tgccgtcggc taccggcctc agggtccaaa cccaaccgtg gaagggacta tcggttgacg
    19561 accgaagccg gttgcgagga gccccagcgg ccactcagaa accatcaatc aggcagggcc
    19621 tattaccccc acgacaacgt gctcgcacgc acgacctggg tcagccccca cagcagaaag
    19681 agcccataga ccgtaccgaa gaaccagggg aagctgatcg cgcgatgcgc ccaccattga
    19741 cgatgttgcc ggaacgccca ggatggctcg aaggagcgcg gctcattact ttcctcgacg
    19801 atcgtttcac gatgttcgcg gagtcgatag agactgctca ccggctttcg ttcccgccat
    19861 tcaggcatgg tctcgatgcc ccgtagctgc cactcccaga gcgcattttg cccggagaac
    19921 cgcccgagca cgatcgccat ctgcacactc atgagaatcc cgatgatgct gaacaataca
    19981 atgatgaggg taaagggaag cgccagcgat tcccgcgaca ccagcaaccc gatcaccgcg
    20041 acaaaaatcg aattggcggt cagatagtcc gacagcatcg tgtgatagtc ccgcacgccg
    20101 gacgtccaca acgtcaatag atgcgcataa tcggatcgca acacatcttg atccagggcc
    20161 atcactttag gctccattga cgtcttgtcc gaccagccga tacgcattgc gcagatactg
    20221 ccccaccatg cgctgcgtgt tgaagaacgc cccgttcacg gcaatcgcat gcttcatgat
    20281 ctcgatgaac ccctcccggt ccttgtaaaa gcaaggcatg acccgctgct caagcttgtc
    20341 gtacagagcc cgggcatggc aggcctccat gtcctccgcg ggcgacacac aggcctcgat
    20401 cttctcgccg atcgaccagc ccgtgacccc ttcgatatgc ccctcgaccc accaaccgtc
    20461 cagcacgctc aaactcggaa ccccgttcat cgccgccttc atgccgctcg taccggaggc
    20521 ttccatcggc gggagcggcg tattcagcca cacatccacg ccggcgcaga ggagacgtgc
    20581 cagcgtcatg tcgtaattgg cgagataggc caccgacagc tgcccgcgca tgccgtgaat
    20641 gcgatggatc atgtccttac cggcctgatc ctgcgggtgc gccttccccg caaagacgaa
    20701 ttgaatcggg cccgcctctt tcaacagccg ccgcaaccgt tcggcatcgt gaaacaccag
    20761 ggtccctcgc ttatacgcag tcgcgcggcg ggcgaagccg atggtgagga catcgcgatc
    20821 gaatcccgcg ttggtctgac ggttcacttc gtccaccaag gccaacttgg catcctgatg
    20881 ggccctccag atttcagcgc cgggaatact gatggcgtaa cgaagcgata attgatcgta
    20941 tcgccagtcg ggcaaatgac ggtcatagag gctttgaaac gagggcgcgg tccaggtcac
    21001 ggcatgcacc ccgttggtaa tggaatggat cggatagccg ggatacaacg agtgggacac
    21061 ctcgccatgc cgcatggcga caccgttgac gaaccgggat ccccgcaagg ccagcaaggt
    21121 catgttcaac gcgccctggt gctgacaggc ctgcacgaga tcgcatcgcc gactccccaa
    21181 cacccgatgc gcgagttcca ccgggaattg gtcgtggccc gccggcaccg gggtatgcgt
    21241 ggtgaacacg cattgctccc gcacctgctc gatcacctcc ggcggcacat ctccgttccg
    21301 cggttccgtc ccgatggccg cctcgatcaa cgccaggacc agcagcgccg catgcccttc
    21361 gttgaggtgg taacgaaaga tgttcccgtg ccccaacgcg cgcaaaagag cgaatccgcc
    21421 gaaccccagg atcatttcct ggcataagcg ataatgcgag tccccgccgt agagcgtgtc
    21481 cgtgatggtc cgatcccagg gactgttttc gggcagatcg gtatcgagca gatacaccgg
    21541 gacggtatgg cccgacatgc cggtgacgtg atgacgccag gcacgaacgt gcacggtccg
    21601 gccctcgatt tcgacggaga ctcgcggctc catgggttcg gcgaaatcgt tgaccaccca
    21661 ggccaccggc tcctccgact gccacccctt ctgatcgagc cgctgataaa aatagcctcg
    21721 ccggtggagc agcgtgacgc cgaccatcgg cacgcccaga tcggctgcgg aacgaatcgt
    21781 gtcgccggcc agaaccccga gtcctcccga ataggtcggc atcagcggat tcaggccgat
    21841 ctccatggaa aagtacgcca cgagggtctg gctgttctgc cgggtcatga ggcctccagc
    21901 acctgcttga tcagtcgaag ctcatcgcgc accagccgcg gcatgagaaa atgccggcgc
    21961 acatgttcac gcccggcccg gccgaactct ccacgttcgc ccggtcgctg cagcagatgg
    22021 agcagccgct ccgcgcaggc ttccacactg tcgaccagat tcttctgaaa gggctcgggg
    22081 aattgcatgg gaatgcctcc ggtgttcccg gcgaccaccg gcttctcctt ccataacgct
    22141 tcggacacga ccaacccgaa tccctcgcgc agggatttct ggattacgac atcacagccg
    22201 cgttggaacg cattgacctc catgctcccc acaccggcga gattcgtaaa gacgaagaga
    22261 tccgggtcgt tcgcggcctg ttcctcgact cgatccagga tctcccaccc ttccggatcg
    22321 tcgcccgcca tcgcgccgat cagtaccaat tgaacggcgg gaatgtcctc tttgaccagg
    22381 cgataggcgc gcaccacccc gagcggatcc ttccagggat cgaaccgcga aacctgcaac
    22441 agcaacggcc tcgtcggatc caccccggag tccgcgatcg cgcgccgaca caagtcttca
    22501 ggcaattcca tgttcttcgt ggccagggga tcgatggccg gggcgatgaa ggccacccac
    22561 ttcgccgcca actgaggtag gacgaactgg ctcatggtaa agacgaccgc ctcatactct
    22621 tcgagcaacg gccgcaaaaa ctgactgacc tctttatcag gcgccgagct gtcgatatgg
    22681 cagcgccaga tccatttggc atcacggggc ccggcaaaat gtcgaatagc cgccggctgc
    22741 gggtcgtgca cgatatacac atcgtagtca ttgccgagcg ccttggcgct gcgttcattg
    22801 accttgaggt acaggtcctg cgccgctttc cccaagccgt agtgcccgcc ttgcagtgcg
    22861 ttatggaacg ccttggtgat ggcaaaaaaa tccgcttcgc catggatcaa gcgccagtcc
    22921 gcctgcacac cgagcgcctg taacatcggc acataccgcg ccaacagttc cgccacgccc
    22981 ccgccggccg ccgtagagtt cacatgacag actttgacgc cctgcagttc tttcgcgagc
    23041 gtcctcagct cctcataggc ctcctcgctc atcagcttcc ggtacccatc cagattggct
    23101 tgtatcgatc cgcatcgtcg tccgatgccg actttcggca actgcgtctt caattccatt
    23161 gtccctgaat ctccctcatg catccgtctc cgaacttcta tcgctgctct tctacccgaa
    23221 cgtcacgcga acccaatcac caaggtagtg cgtgatcgcg accacacaga gtccgatcac
    23281 gacatgctcc ccgatcacct tccagggcgg gacccgttgc gcgcgcgcca gcgcataact
    23341 gagcaccgcc aacagcgaca acccccagac gaggctggcc gctacagcca gatccaacgg
    23401 catgaacaac accggaaccg caaaggtcgc ggcaatcacg aacttcgccg cgaaggtcgc
    23461 cagggtcgat tcccaaattt cattcgtatt cccatgattc ttggattcct ccgcgatgtg
    23521 gatgcccaac gcatcggaca tggcgtcggc gaccgcaatg gtgagcagcc cgcccaggac
    23581 cacggacttc gaatgggtgc ccgcatgcag tcccaccatc aaccccaacg tggtgatcac
    23641 gccggaggtg agtccgaagc tgaacccgat cttcaacgcg gtgcgcatcc ccctaccttg
    23701 tcatcgcagc agaccgtctc agcaggatga ggaaaaagtc cgccagcggc gttctcgcgg
    23761 cgctcagaag ctcaccgtac agcgcgagta cgattcgcct cttcgctggc tgcggccttt
    23821 ctggacggcc ttttttcgca tcctgctggt tattgtacta ccggcacggc gcaagagttg
    23881 atcgtagcgt attgcgcaac gaccgagttt ttccgcggcc tgctagagtg agcggaccgt
    23941 cgacgtcttg gatcaacagg cgtccttccc cgctcctcgc aggcaggatg agatccagtc
    24001 attcagcgat tcaagcaccc gggtcaccgc ccgattggcc gccagcacgc ctccataggc
    24061 atcctcactc ggagccggct cgaacgattc aagactcctg gcgcccatga tgcgttgctc
    24121 cttcgtttcc gtcagttggg cgcgcagccg tatcctagtg ccgctcggcc gctgcagaaa
    24181 ttcctgttgc accaccaggc cggaaatgtc cagccggtaa tctccccggg ccgccgtcgg
    24241 catcggtacc accacacgcc agaagctcat acgctccagc gactgcagca gcaagggcgc
    24301 caacatccgc ggcggggtat cgacccacac attggttgcg tagtagttca cttcatacgg
    24361 ccgctggagg taggccatac gcggctgttc gaatccggct tccgcctgcg gcactcccac
    24421 gacgaggacg ccatgagtac ccgctggccc cgttcgaggg ttcgccccag acagagactc
    24481 gtccattgcc agcacgaacg tatgaaccgg attgtctccc gatcgcggca cgacacaggc
    24541 cgtcaacgcg caggaccaca gcaggactcc gacccatttc atgacccgcc ggttcacgcc
    24601 cacgtgtccc tcactcgccg ggccccttcg tttgggcgga tcggccaagg accagcacat
    24661 tcggttgccg ttcgacctgc tgcgccacac gattcaaggt ggcggtcagt tgccggagct
    24721 ccgtgacgag caaccccgta tcggccagcg tctgccgcga aaactgttcg atgccgggcc
    24781 gactggcgcc gaccatctct ccgaccgagc ggcttgtccg cgataagtct tccgtcatct
    24841 gctgcaatcc agccgcgctc ctgctcaggc gctccaccaa tgcgggaagc tgtctgccga
    24901 gttgctccgt cacacgcgcc gcctgatcgg ccgcctgcac cgcctgctga actccgcggt
    24961 ccatctggcc gctgcgttcc gccagcactt ttgtgacccc cgacagatct ttcaggatct
    25021 gcttcagggc cgtacgattg tcttcatcca gcgcagaaga agcattttga gccagcccgt
    25081 tcaaattggc cacgagggtc gacaagcctt gatccgacaa cagtttcgcc agcgtcccgt
    25141 ccagacggcc gaatagcgag ggcacgctct tgatgaccgg atagtcttgc ccggcaatcg
    25201 gtgtcaggag aggcgcctcc cgggaaccac cggtcaaatt gagggtgacc aaacccgtga
    25261 gcccttgcgt gaccagcaca gcctgcgtat cggtcttaac gggtgtcccg acgacgatgt
    25321 ccagagtcac ccgcacctct tcggcattct cagggttgag gatgacttgt ttcacacgcc
    25381 cgacatccac cccgcgatac ttcacggtcg aatcgacgct gagtccggcg acggactcac
    25441 gcgtatagac gtaataccga tcgtagatcc cgcgataatc ggttttgccc aaccagaaaa
    25501 tcgcaccgag gatcgtggcg cccagcagga cgacgaacgc gccgaccagg atatagttca
    25561 ccttcggttc catccgccac ctctcaatcc cgatgcagtc ccgttccgga atgattcaga
    25621 tgggccgcac gccctcgggg accgtggaaa tagctccgga taacgggatc gtccgaacgg
    25681 gccagcgcgt ccatcgttcc caacccgagc acctgccctt cacccagcac cgccacccga
    25741 tccgcaatgc gccacaacga atccagatcg tgcgtcacca tcacaacggt cagacccaac
    25801 aatcgtttca actccagaac cagatcgtcg aatccggcgg caatcatcgg atcgagtccg
    25861 gccgtcggtt cgtcgagaaa cagcagctcc ggatccataa cgatcgcacg ggccagcgcc
    25921 gcgcgtcttc tcatcccgcc gctgagctca ctcgggaatt tgatcgcgct gtccggaggt
    25981 aatccgacca gggcgatttt gagcgccacg atatcccgaa tcgcttcagc gctcaacggc
    26041 gtatgctcac gaagcggcac cgccacgttt tccgccaggg tcatggaact gaacagggcg
    26101 ccatgctgga acatcacgcc gaaccgccga tgcagcgggc ttccgttcga atgtgtcagc
    26161 tgacgactgt ctgtgcccaa cagacgaatc gtccccgtcg tcggagccag caatccgatg
    26221 atttctcgca gcaacgtcga tttcccgcaa ccgttgccgc ccgcaatggc gaacacctcg
    26281 ccacgcacga ccgtgagact cacatcccga tgcacgaccg ccgtgccgaa ccgcgtcgag
    26341 acatggctga cttcgatcac gggcgtctca attcgcacgg ccgtagacgt cattcctgtc
    26401 actccccaag attaggtctg gccggagaac ggatttcaaa cattttggta gcccggacta
    26461 ctcatgtgta ccgtcgcgag gcaccctggc tataggtccc accagttgag caagatgctg
    26521 caaatcgcat cgaagatgat gacgagaaaa atactttgca ccacactgat cgtcgtatgc
    26581 cggcccacat catccacccc gccgcgaatc tggaacccct gataacagcc gaccagcgca
    26641 atgatgacag caaagaaggg cgccttgccg agaccgatga gcatatgccg tagcgccacg
    26701 gcctcctcga agcgcgagag aaaggcggtg aaactcacgt tgagctgatt cgacgcgatc
    26761 aacatgccgc cgaagacacc cagcacatcg gcataggcgg tcaggagcgg cagcgccacg
    26821 accaacgcga ggacccgcgg caacaccaac agctcgatcg gcgacaggcc gaaggtttgc
    26881 agcgcatcca attcctcagt caccttcatc gtgccgatct gcgccgcata ggccgacccg
    26941 gaccgcccgg cgatcagaat cgccgcaatc aacggggcga tttcccgcaa cagcgagata
    27001 cccaccagat ccacgataaa aatattcgtc ccgaacttcc gaagctgctc ggcgccctgg
    27061 tacgcaatca ccacgccgat gagaaacgtc agcagcccgg tgatcggcaa cgcattgacc
    27121 ccgtccagcc ggaggctgtt caggcaactg cgccaacgca tgcgaccggg cgacgccacc
    27181 aaacgaacca gcgccgtcgc actctccccg agaaactcca gcgcccgggc accctgggcg
    27241 acacgatttc tgatcgacgt ccgaaaccgc tcagtcccgt tcgaccgcga ggcgacctca
    27301 gccacaatcg cgccccaatt ggacgacacc aggttcatca gctgcgcata gtccggccga
    27361 aggccttcga tcgagacctc gcagccctgg cgtctggctt ccatcaaggc gcgatacaac
    27421 acgaccgcac cgccggtgtc gaacgcctcg atcccgctcg cttcgaagac caacacactt
    27481 cgagcaggcc agaccattcc tctgagcgca cgttcgaccg gcgccacatc gggcatggtc
    27541 caggctcccc gacaacggag ggtccggccc tccagccacg tcacaccggc cggctgctgc
    27601 tggttagcca ttagttgacc gcgcttcatc ttccgcttcg actcgcgcct tacacctgct
    27661 tctgttcgcg caacgtctca atgagttctt tcttactgac cagcagcgca cccagcccga
    27721 acagcaccgt caacaacgtc aacagccctc cgaccaccgg aataagcgac agcaccgaat
    27781 acaggaccag ccccgccacg aagggccagg caagcgacga cgaatcctcc tgccggcgaa
    27841 tcacgatttg tcccacatag gtgatggcat agactctcgc cagatagacc gtcgcgccat
    27901 acaaggcgaa cagcaacagg ccgatcggaa gtgcgaacaa tgtgacgacg aaactcagcg
    27961 ccaccacggg cgtggcaacc agagccaccg tgccccaacc gagagccgct gtcggacgtt
    28021 cacggatcgt cgcggtgacc cgcctggcaa acaccgggta gacccgtaag agcaccagtc
    28081 ccaggatcaa cgtcgagacg aatccgatga atgcagccat gagccggatg ccgacgagcc
    28141 cctggcgcgc ccgttcgatg ctccaccctt cgggcaacgg ccgttgtgta atcgcgcccc
    28201 gaacggtcgc ctcttcgtcg attgaggggg ccgcttcacc ccagtacctc agcctgcccc
    28261 cgaccgtggc cttcgaggtg agacgcaccg accccgccgc cacgatcaaa tctcgctcaa
    28321 tctggttcga gagcgtcgct ttccaggcac cgaccctcgc atcccgaccg acagacccgg
    28381 caagttgcac atggccaccg ccggcgatga ggttgtcccg cacctttgcc gtctcggtca
    28441 gatgcacatc tgcgccaccg agggtggcat tcctcccgat ggttccactc accgtcacct
    28501 gcgcaccggc aattctggca tcctgagcca cggtgccgga cagaatcact ttggctcctg
    28561 ctacgatcac atcgccgttg accactccat cgatcaacac ctcaccaccg gcggcataga
    28621 gatcgccgtt gacgatgccg gaaatttcca catgcgggcc gaaggcgaaa tagtccccct
    28681 gcacaatctg ccccgcgcgt aacacggccc gctccttcca cggcgcgcga gacaacggtt
    28741 catcggcctg agccgtggca ggcagcatgc acaggatcaa caacatcacc atacgtgtcg
    28801 tcatgtgagt tccctccgaa agcgccgcag gccatacccg acctggaacc gcgctcatcg
    28861 tggcaggacg ccggcgccga atcgatagac caggtccccg ccgaaataac tcgcaatcaa
    28921 caacaacagg ccgccgccca ggccgatgat cagcaccagc ctcggctgtt cgcgaatcca
    28981 tccccaaaag gaggccagcc gcagcccgag caggccgaga aatatccaga acaccgagcc
    29041 gcccagctcc tcatgaattt caatggcctg tttcgggatc ccgctgcgtt ccgcggcctc
    29101 ttcggcaaaa tgtccggtca gcactgccgc tccggctgcc agaataccga ggatcaggag
    29161 agataaactg gtctcccgaa actgctggct ccgccatcga aacgccaacg catcgaacag
    29221 ggtgcttgcc aacaacaatg cgatgggaaa atggacgacc atcggatgaa tgggatgcat
    29281 gccctctcct tcgttgcaca cagtaattcg gcacggcctc atctggaatc gtcaaaacgg
    29341 acaggaccgt ccaagattgg cgccttccaa tccagaagtg caaggatgga gccctactcc
    29401 ttgcagacac agtggcatgc aaaagcttac gacaagaccg gagcaggaga ggcgggagtt
    29461 ccagaaggcg gggaaaaatc ggggaggact gctacagagc gactccttgg ctctgtggca
    29521 gaatgcacca gaaccgatgg atcagaacag gagacagaag ctgccttcgc atgaacggct
    29581 cgtcgttcgc tccgctcaca gcacccgagc tcaggtctgc gcctgtacag aaagcaggag
    29641 tagatgtttg cgaccgggtt gccgaaactg ctgaaccgtt ccaacgacca accccgcttg
    29701 tgcctgcagc gcagcggcat gttgctcctc agtcaccacc cggatggccc catgtgtgcg
    29761 cactgcttca agcaccgcat cctgactcgg tagatccggc accacattgt cccgcttcag
    29821 ataatacagg gcatcctcat tcttgggcca cccggggata tacatgaaca gggcgggctg
    29881 agttcccaga gccagggatt tgatttcctc cacagccttc cggggcgacg cggcctgatc
    29941 gatcgcggga aacacgtagt tcaccaccag caccacgtag gcgatcgcca gggctccgac
    30001 catgccgagt gccaggcgta cgcgtgatcg aagcacggca tagaggagca agccggccag
    30061 tgaaacgatc gtcacgatga acatcggaga caccacggca gtggacacca gccaccggcg
    30121 gcgaagcggt gctgcgccaa agaacatccc ggcggcgaac gtcacggcca acacacccag
    30181 caacaggcgg agcaggggcg ccatcgcagc agagtcctcg gccgacgaga acagccggtg
    30241 atagtagtac ccgatcatca agccaatacc gggcacgatc gtcgtcaaat agggctcccg
    30301 tttgagtgtc gccaagctga acgccgtgaa gaggcccagc acccagacga gcagcagcag
    30361 ctccgtggga tgagcacgaa acggtcgttg ggcccagagc aagagcagcg ccgacggaag
    30421 caccgcgcac caggggaaaa agtcggccca catcatcccc agataccaat acagcgggtg
    30481 cccgtcgatg gccgggccgg ctttgccgat gaatccggta ttccaagtcg attcgatggc
    30541 tttgccgatt cgaaaatggt gctgataccc gggccccagc atctgcgcgt atgcccccat
    30601 catcgcgaca gccaccgcca agccggccca gaagtatcta tctttcagca ttcgcgtatc
    30661 gcgctgaatg gcggcataca gcgccatcac gagcaacggc agaaaaaacc cgtgcatctc
    30721 tttcaacatc gcaccgagag ccatgctgac gaaggcgagc agataccagc gtgagtttcg
    30781 ccccagcagt tgcacctgaa tccacgcgaa gagggcgagt gtgaccagaa atgtgagggt
    30841 cgaatcgaac agcacgcgac gcccgtacca gagaaacaca tggctggtgg cgacgaccag
    30901 cgccgcccag aatcccgcag tcggagacac cagcctggct ccaagcacat aggtcagaac
    30961 gatggtgccc acggcggcaa gcgagccggg cagccgcaag gccacttcgt gatcgcccca
    31021 tattgatgtc gagagattga gcaaccaaaa gaacagcggc ggcttattga agtacggcac
    31081 atcgtgatag gtcaggtcga aaaattcacg ccggcgcccc atttctcccg caatgccggc
    31141 atacagtcct tcgctgcctt ccaataacga cagatggaga ttggcaaaat aggcccagcc
    31201 gccgaccagc gccagcagcg caatttcaac gtaccggcga atggcggcat tcgactgcgg
    31261 acgttcatcc gttccgtcct gattcatcac ctgttcgacc aacacctcaa cactcccccc
    31321 gtttccggat tatgcccacg cacgtcacgg ctgttccatc acaagcgcct cccgaagaag
    31381 acgatcctgt cgaacgacgt ataggacggg tttcttcgcc tgcgcagaat gatgcaccag
    31441 gcatagaacc cggcgcttcg atccggcgcg aaagcatgcc agaccgacgg gtaaatagcc
    31501 agtgcgccgg cggcgctctg atgcaaaatt cgtcacgcgc ctgtcactgc tgacccaatc
    31561 ggtctctcac agcaacttcg tggcttcggt cgggcgcgga accttgacca ccagcacgcg
    31621 aaacggcttg tcgctccggt tggaccacct gtgcggaatg cgagccgggc tgggaatgag
    31681 catgtccggc ccgacttctt tcgtgtcctc tccaatctcg acgatccctc gtccttccag
    31741 gacatagaaa aagacatcga cgggtgtcac gtgcttcttg agcgattcac ccggttccaa
    31801 cgtgatatga acgacctggg catgctccgt gtcgtacaac cgactcgcct tgacctgatg
    31861 aggattctga gtgagatcgg cctctcgtgc gttgacaatt tccatcattc gctcctttga
    31921 ctcaagaatg tccgacgttc gtgaggaggc gctcggctgc cgagcggcca cagtgccgtc
    31981 tactaaaacc ggaatctgat ccctccgccg aacccgtaat tcggcgcgcc gttcgacaat
    32041 ccgaccagca acgaacctgt taggtgaacg gtgtcgttca caatgtagtt cgtcagggcc
    32101 aagaggtcac gggcattgtt cacggtattg accaatgcgc ggtattcttc ataaaagaga
    32161 ctcatgtgga gattgtccgt aacgtcgtat ccgacgccga cgtcatacca ccactgattg
    32221 ttgaagttgg tgcccggagc atcgccgatc aggttatagc ccccgtcaag ataggccagc
    32281 catcgatcac cgagactttt ggtcaattcc aatcccgccc cttcatcgaa ctcgcccgtg
    32341 cccagcccga gatcggcatc cgcagtcggt aatttgaccc gccccgtcac cgccacaagc
    32401 ggcatgacgg cgctctcctc gatcaggtaa taacgaccac gcagaatcac gtcgcctaga
    32461 ccgctgtcgg ttgtggcgct ggataacggg gcggcgccgg gccctttgcc cctgcctgcc
    32521 ccgccggcga gcgccgccgt gggactgacc gcggcactgc ctgtgcgggt cggcattccc
    32581 cccacgagtc gaaccgctcc ggtcccggag atgctgacaa agggaatcgt caggctgaga
    32641 tctccgtctc gaaacacccg ccgaaccgtc atgggcgcat agagaatgtt cgtcctggaa
    32701 tccgcgccgt acgaaccgct cgaataattg acggtcgtgc tgacccgcca ttgaggggtt
    32761 ggttggggcg gcccttcgac ttcagccgcc gtccggtcaa ccgtccctgc gccgaggaga
    32821 aagagacccg tccccaacaa aaccgctgtc aggcccacac cgctgtaacg ctcccttgcc
    32881 gcaaggatcg cgtcataccg catcagattc cccgccgcag tcaacggccg ccgcgttcgg
    32941 tccgctcagc cttctcatgc tgcggtctct ccggacgctc cgtacgagtc tgttcccgcg
    33001 tccgctcgcg cgttcgctcc tgctccttga gttgctcctt ttctttcagc tgatccttcg
    33061 tctgcagctt gtccttatcc tgaatttgtt gctgcgtttt gagctggtcg cgatcgcgat
    33121 cctgcgccga cgcaataatc acatttcctg ccgggaatga cagggcaacc gccaccgcga
    33181 tggaaagcat ttttctgagc ttcatggggc acctccttgt gcatatagcc gcgagcgata
    33241 ttgctcacgg cagagagggg cgatgagcac gctccgttgc gctccacatc cggccatcac
    33301 cgtcacattc gacagcctgt catcgtccat caaatgggct ctgccttctc cacgcgcact
    33361 ctcacattac atgctattgg ttgactccaa atcgatagag atcgtgacag gtcgcacacc
    33421 gcgccgtcat gctcgataac cgctgaagaa tttgctgcgg agtttccttc tgcacgatcg
    33481 catcggccaa cgcatccatg tccttatgga tcgacaggcc catctgtttg aacggcagcg
    33541 gcagcttcgc catgatagcc ggttccgtgt ctgccgccgc cttcatcccg gccgctcttg
    33601 cggctgcttc catcgcctta ggatccggct gcggttcgcc gagccctctg acgactccat
    33661 ccaccgcttt gaaaagttgt cgcatttcgg ccaggatatg gtcccgctcc gccggcgcca
    33721 gcccgatctc ctgccgtccg tccgtgcccg gttttgtcca tccagctaca aacatccacc
    33781 cggcgacggc cagcgtgacg atccagagcc caaccattac ccggcacagt gtgtgtccct
    33841 tgctgcccat cggtgacctc ctgtcacgca cacaatccct ctctcctcga acgacgttca
    33901 atcatcaatg aaactctctc tttgctgtaa gagcaggatc cgtccggaag gattcaccgt
    33961 tcggcggcct gtccggcgtt tcattctaga tcggccggcc tgaaccgaga gtcctggaca
    34021 atgctgagtt atggcagaac gtgtctagcc ttgtgaggcg atcttagggg aggccccggg
    34081 aagatgggtc cacgcgcacg aaacccgtcg gccccttctg tgagtggcct agaatgccaa
    34141 atagaatcca aatccgatac tctcgacctt ctcgctaaag aactgtccat agggataaaa
    34201 gccgcgataa taattcacga ggaaccgaaa ccgacgcttc gcgccgggcc tggaccactc
    34261 gaacccgcca atgacattgt ggttgacgat ccatcgcagc tcttcgaagg tcttgaagtc
    34321 ggcccccagc accggcgtga gctggagccc cggcatcatg ctcccgagga acggcgagtg
    34381 aaccgtcggc ccccgcagct ccatgcccca ctgcactcga tttcgatcca gatgcgccgg
    34441 ttcgcgatga atcaaatacc ctccgcccgc atacatcctg acccatcggt actcatagga
    34501 aacgatcccc tcaacttcct cgaaactgag attcacccga ttgaatcccg gattctcgag
    34561 aagaaattca tctccgacat ggctgctctg gtggtagagg cgcactcgcg tggaccatgc
    34621 cccatgccgc caggagagcg gaattcccac gatataatcg gcattgatca ggtccgacga
    34681 tggagcatcc agattgaatt gcgaaaagac tcccgccagc aagcccacct gcgacccatt
    34741 acagccttgc cgcttggtgt agaagccaaa attttctccg aaccccaccg agccaacgtt
    34801 cacggacttc ccgacccctt ttacagtact cgtgggttct ctgcgttgaa cggactgata
    34861 gctggcgaag aactgcggct gcttcggatc cgccatcaac ggccggaata catcatcgcc
    34921 tgggaacgcc gaactactcg ttccactgtc gctctgctcg taccggcagt cgatcacctc
    34981 gccgttcttc atgtccggcg cttcttcggc ccggacttcg cccaccgtca tggccgtcaa
    35041 accgatcacc ccgatcatcg ccttccagaa catatccatt ccagcctagc ctatggtttt
    35101 ttgaaaacgc ctcgtcgcta cccagagcgt ggtcgcccct aacatcaaca gcgctgccat
    35161 ttgaggccag agaatgtcag gccccacacc tttcaggaaa atcccgcgga tgatcacgag
    35221 gaagtagcgc agcgggttga ggtaggtcag ccattgcacg gcttccggca tgttcgcgat
    35281 cggaaagacg aagccggaca ataacatggc ggggaaatag tagaaaaagg cgctcatcat
    35341 tgcctgctgt tgcgtccggc tgatggtaga gatcagcaag ccgatcccga gggtgctcat
    35401 cagatagaga ctggttgccc ctaagagcag catgagacta cctctgatcg ggacgtcgaa
    35461 ccaataaccg gcgatcgcgg taaccagcac gacatcggca aaaccgatca gtgcaaacgg
    35521 caacatcttg cccagaatga actccgactg cctgataggg gtgaccatga tctgttcgat
    35581 cgtgccgagt tccttttcac gcactaccgc catgctggag agcatcagcg tgaccagcgt
    35641 cacgatcaac acgatgacgc cagggacgta gaagttgcgg ctttccagat tctcgttgaa
    35701 ccaggcacgc gtctccagcg ccacgcgggg cggggcggcc ggctgacccg tcgctcgcac
    35761 gaagcgagtg tgcagcacga cttcactgaa acgaccggcg atctgcccgg catagttgag
    35821 cacgatgccc gccgtgttgg agtccgttcc gtccacgatc atttggagcg ccgcggtccg
    35881 gccggcgcgg agatcctccc cgaacccctt gttcatccgc aggacggctt tggccagccc
    35941 atgatcgacg aggttccggg cctcttcttc ccgatcgacg tatcccacca catcgaagta
    36001 gccggaaccc gtgaatcgcg caactaactc gcggctggcc cggctgttgt cgaggtcgaa
    36061 tatcgcggtc ggaacgtgcg tgacgtccgt ggtgacggca taaccgaaca ccaacgtctg
    36121 cacgagcggc atcacgaaaa tcacggtccg cattcgggga tcacggagga tctggatgaa
    36181 ttccttgatc agcatctgcg tgatccgctc gaacatcact gcctcacgcc atcttcttct
    36241 tgaagagcaa attcgcggcc aggagtacga ctaggccgaa gccagccagc agcgccgcct
    36301 ccagcatgag taactccagg ccgatccctt tgagatagat cccttttaac aacgagacga
    36361 aataccgggc cggcaccaca tgggtgatgg cctggatcgc gttcggcatg ttgctgatgt
    36421 cgtacatgaa cccggacagc aggaacgccg gcaagaaggt gacgaccatg gcgagttggc
    36481 tggccaataa ctgggacttg gtcacgatgc tgatcagcat gccgagcgaa agcgcgccag
    36541 ccagaaagat ggcggccatg ctgaagagga gtgcaacatt gccgcgcagc ggcacctcga
    36601 agacgaactc acccatcagc acggcgatca gcacatccag catcccgatg ccgaagtacg
    36661 gcagcagttt gccgaggatc agctcggggc ctttcagcgg cgtggagatg atctgctcca
    36721 tcgtgccggt ttcccattcc cgcgccacgg ttaacgaggt cagcatcgcg gcgatgacca
    36781 tcatgatcac cgcgatcagc cccggaatga tgtaattctt ggattccatg tcggcgttga
    36841 accagacgcg cgggcgcaga tccagcggga ccgtgacggt ccggccgctg ctgcgggcgg
    36901 cttgctcgat cgccaaatcc tggttgtagg tccgcgcgac ggtctcgaga taccccaata
    36961 cgatcgtcgc cgtgttggag tcgctaccgt ccacaatcgc ctgagctcgc gcagtccgac
    37021 cagaccctac atccatcgcg aaatcgactg ggatgaccaa tgccaccagc gcctcccccg
    37081 catcgatggc ccgctccacc tctgcatagg tctgcacaat acgacggagg gcaaaatagg
    37141 gcgatccctc aaagcggctc atgaagttgc ggctggtctg cgtgccgctc tgatcccaga
    37201 ccacgagcgg cacctcgtcc acgtccaagg tcagcgcata gccgaacaga atcaacaaga
    37261 ggatcgggat cgcgattccc atgacgaggc tacgccaatc ccgaatgata tggatgccct
    37321 ccttacgggc gaccgcccag atgcgccgag gcctcatccc ttcacctccg cctgtggccg
    37381 ctcctgtcgg tcccgcgcct cgattaacga gacgaacaca tcctccagtg acggcacgat
    37441 ccgttccacc cgcgacacgg caaagttccg ctccgccaac cggttcgtga tcgccggaat
    37501 cacgcctttc gcctgacgct cgaccaccac atgcagaccc ttgccgaaca aagcggcttc
    37561 cttgaccccc tcgactgttc ccagcactag gatcgcctcc tgcggctgct cgcagagcac
    37621 ctcgatgatg tcgtcctgca tgaagcgcgt cttcagctcg tccggcgtcc cggaggcgat
    37681 caattccccg cgatagatca accccagccg gtcacagtac tccgcctctt ccatgtagtg
    37741 cgtcgtgacg aagaccgtca cgccttgtcc ggccaactcg taaatcagat cccagaagct
    37801 gcggcggctg atgggatcca cgccggacgt cggctcgtcg agaaagatga tcggcggctc
    37861 atgcaggatg gcgcagccca gcgcgagccg ttgcttccag cccccggaga gcacggcggt
    37921 gcgtgagccg cgatgctgcg ccaggcctgc catcccgatg acccactctt tccgctcggc
    37981 tttcttcgcc ttgggaatcc ggtaaatgcc gctgtagaag tcgatgttct cctcgaccgt
    38041 caggtcctcg taaagcgaga acttctggct catgtagccg atgtgggtct tgatccgctc
    38101 cgcctcccgc ataatatcga agccgtcgac ggtcccggtg ccggccgtcg gcgtcagcag
    38161 cccgcacaac atccggatcg tcgtcgattt gccggcgccg ttcggcccga gaaaaccgaa
    38221 gatctcgccg gttttgactt gcaggctgat ccggttgacg gccaggaacg agccgaaccg
    38281 tttttctaaa tcctggacaa cgaccgcgta gggctcacgc tctggcatca atcggttcct
    38341 gcggagggac ggcctcttcg ctcgtcaata ccgaaacaaa gacatcttcc aaggacggct
    38401 caatcggccg caggccgtgc agcgtaaatc cttcggcctg cagcacggcg gacacttcct
    38461 gcgcggtctg cacccggtcc atcgtgacca catgaatgcg gtcgccgaaa agcccgaccg
    38521 actcgacctt cacccgccgc ttgagcaggg ctgcggcctc acgcgctcga ccgacacgaa
    38581 cttccaggat cgtgccccgc atgagcgtct tgacccggtc cggtgtatcg caggccagga
    38641 gacggccttg gtgcagcagc gccacccgat ggcatcgttc ggcctcgtcc agataggcag
    38701 tggagacaaa gatggtcaca ccttctttga ggagggaata gagaatccgc cagaagtccc
    38761 gccgcgagac ggggtctacc ccgttcgtcg gctcgtccag gaacaagact ttcggcgtat
    38821 ggatcagcgc acaggccaga cccaatttct gtttcatgcc gccggacaat cgcccggcct
    38881 gccgcttctt gaacggggtg aggttgctga acgagagcag ttcgtggatc ttgtgctcga
    38941 ggcctttcgt gggcactcca tagagatccg cgtagaattg gagattctcc tccaccgtga
    39001 gatccggata gagcccgaat cgttggctca tgtacccgat atcctccttg acctcctccg
    39061 cttctcgaac gacatgcttc cccatgaccc aggcctctcc gccgttcggc tccatcacgc
    39121 cggtcaagag ccgcatcgtg gtcgtcttgc cggcgccgtc cgggcccacc aagccgaaaa
    39181 tttctcccgg ctccaccgtg aaatttagat cctcgaccgc gcgaaccgtg ccgaatgatc
    39241 gcgtcagatg gacgagctta atggcttcca ccggatgcct ccgcgccgtc cagaacgatc
    39301 cgggcatcgg ccggcatccc ggctttgagc tccatgtgag gattgtcgac ggtgatcttg
    39361 gtgcgataga ccagcttgac ccgctccttc ttggtctgga cgctcttcgg tgtgaactcg
    39421 gcctgggaag caatgaaaga cacgcggcct tcgtagacct tgtccggata ggtgtcggct
    39481 gtgaccttca ccacctgtcc caccttgacc cggcccaagt ccgtctcatc gatgtaggcc
    39541 cgcagccaga cgtgcgccag atcgccgacg gtcacgatcg gcgtgccggg agcgacgaac
    39601 tctcccggct caatgttgtg ggacaacacg acacccgtca gcggagaggt cagcgtggca
    39661 taacccagcc gggtctcggc cagggccaac gcttccttgg cctgctgcag ctgcgcccgc
    39721 gcccgctcga ccttttcgat gcgcggacct ttctggacaa ggcgcaattg ctcctgcgcc
    39781 tcgcctagct tcgcggtcgt cacctccacg gcgctcttgg ccgcgtcgta gtcctgcccc
    39841 gagacattgt cctgctcata gagtttcttg aggcgcttaa aatccgctcg ggcgcgtgct
    39901 acatccgcct gcattcgccg gacgaccgct tccgattgcg cgatttcctc cggccgagat
    39961 ccggtcacta actcggcgag cgaggcctca gcggcccgaa cttccgcgct gcgaagcgcc
    40021 acttcctgcg ccagctcact ggtatccaga cgagccaccg actgccccgc cttgaccgat
    40081 tccccttccg acgccgaacg ttccactacc cggcccgcta tcttgaagct cacctcggcg
    40141 tcggttatct cgatattgcc cgatactcgc agcactcccg agtccggcac cggtcgtgat
    40201 tggaaataca ggccagcggc gacggcaagc accagcaggc tgaggatcag cagcgcccgc
    40261 atccacgcag taggaatagt gcgtcctgcg ccgggagctg cggcaaccgg cggcgaagct
    40321 ccatggccaa tcggcccgga gtcacccgtg cggggaggaa tcgtaatcct gtctccaccc
    40381 atgatcgtcc tcatccctca aatccgtgcc atcacagact ccgttcacgt cgaggcgtct
    40441 gccgtgcgcg gattcagaac aattgttcga tttcgccttt cgctctggcc agattgatgc
    40501 gagacgcatt gaagcggaac agcgcttcga tcagattgtc acgcgcccgc gccaccgagg
    40561 tctgggcgtt cgtaatttca atattggtcg cgagtccggc agcgaaacgg tcccgtgcaa
    40621 aggtcagttc cttcatcgac agggacaatc ctttctccgc caccgtcact tgctgcgtag
    40681 atgagtcgag cgtgagcagc gcatttcgga cttcgagtgt cacctgatcg gatacgtcct
    40741 tcatgcggat cagttcctgc cgcacacggc tccggctttc ggaaatccgt ccctcccgtt
    40801 gtccgccgtc gaagatgggc accgagagca tcagcccgac ggatcgcgtc gccaaggctt
    40861 catccggctt gagcccgatc cacccatagt cgccgttcaa tgacaacgag gggattcgct
    40921 cactggtcac ggaactgaga ctgagcgacg ccagcttttg gcgcgtgagc tgcgccttca
    40981 gttcggcgcg attctcgcgc gctaccgaca gggccgtttc gggggtttga gattcgactt
    41041 tcgccatcgt gagttcgtcg gtcaacgtca cggtgacatt gaaatcgatg cccaacgtgc
    41101 gaatgagatt caacttcgcg ctttcgtgat cgttttgagc gacgagcagt cgctggcggg
    41161 tgttctctac ctgaacttct tcgcgggtga catccagccc ggtagcgaca cccgcctgct
    41221 tacgatcctg cgccagtttt aacaattgct gactgagttc gagatccgcc agcctggcct
    41281 ttaccgcagc ctcagcacga gaagcttcca tatacagaag gcccgccgtg gccatcacgt
    41341 cgcgcttggt cacttcggcc tcaagccccg ccacatcgac tccggttttg gccgcccgcc
    41401 agcgttggat caagctcagg ctgaagacat tctgcacgag gttggcccgt gcattgtaca
    41461 catcgaacgg ttcggttacg ggtctctgca gtcccaagcc ggagagtcta tcgggaggaa
    41521 gtccgaacgc agcgagattg acggtttgat tgctcccgct catgaacccg gacacgttcg
    41581 gcaataacgc acccaagctg gtgctggccg ccgattgggc cgccacgatt cgctccttca
    41641 acagccgcac attggcgtta ttgtcgatgg cggcctgaat cgcgtcgtga aggctcaggc
    41701 gcaactcagg gggaggcaac cacgactcag ccctcaccaa actcatcatc cagaatgtgg
    41761 cgagtattgc gatgcccagt gttcgcttca tcatcggtcg ctccattttt tacgttctac
    41821 aggatgacgg tgatcgtggc caacgccgct ttcccgtgtc ggcaattttc ccgatgcaat
    41881 gccccgtcga tagatggcaa acgcacctgg cgcttggcgg cgaatctgtg acacatttcc
    41941 agtgagcagc gactgcatga ccagcccctg aatggtcccg acaaacagcg ttgcagcagc
    42001 ggctgaatcg aggctcgcat cgagctggcc ctgcttcttg ccttcctcca tgagtcgaat
    42061 caatcgctcg gtgtaacaac ggagcaaggt ccgcaccatt cccttggcga cagtgtcttc
    42121 ggatcgctgc agttcaccga tcagcatccg aggcacccca ggatgctcgg tcacaaactc
    42181 gatatgggtg agaaagatgg cttcgacagc cgtgaggggc gaggaagcag tttgcgcggc
    42241 tttgtcgatt cgtgcgagca agcgttccgc cacccattcc atgaccgcct ccaagatagc
    42301 gtctttgctg ggaaagtgcc tgaacagagc gccctgggtc agtcccatcc gcttggcaac
    42361 ggcggcggtg gtgatatcag tcggattctg ttccgccgcc aatgcaatca atgcccgcac
    42421 cgtgtcattt cggcgttgtt ccgccggaag gtgcttagga tgagccatac cgcctccatc
    42481 aagatagata gtaattaatt actatcttcc ggggcgtcat gttgtcaatg tgagcaccta
    42541 cggcatatcc aagacggaat taggcggctg cgtgcgaagc aggggtatct tgctacaacc
    42601 ccggccaaga gtctgtagcg gaaggcggca gaccggcagg acagaatcag ctccggctca
    42661 ttccggttgg cttcccgcaa ccggccgcgg cagctcggtg tgttcctgtt tcggtaacag
    42721 gccggcaggt gaatttcgag ggccacccat ccacaggtag tagagcacag gaatgacgac
    42781 cagcgtcaga accgtcgagg cgccgacgcc gaacagcagc gagacggcca gtccttggaa
    42841 aatcgggtcg aggataatca cgaaggcccc gaccatcaag gccgcggccg tcaacagaat
    42901 gggacgcgtc cggatcgcgc ccgcgcgaat cacggcctcc aacagcgaga ccccctccgc
    42961 ctcctgatgt tgaatgaaat cgaccagcag aatcgaattg cgcacgatga taccggcgag
    43021 ggcgataaac ccgatcatcg acgtggccgt gaaataggac ccggtgagcc agtggccggg
    43081 aagaatgccg ataagcgtga gcggaatggg cgccatgatc accaccggcg tgagaaacga
    43141 ttggaactgc gccacgatca gcaaatagat caacagcagc gccaccgcaa aggcgatccc
    43201 catatctcga aatgtttcat aggtgatgtg ccattcgccg tcccacttca tcgcaaaccg
    43261 ctgttccgac cagggctgca cggcatactg ctgggtcatc gcataccctt caggcaacac
    43321 cagctgctcc agcttcttcc cgatgcccat cacaccatag accggactct cgcctttgtc
    43381 cgggcccacg cctcccacat ccgcaatgac atagaccacc ggcttttggt tcttgtgata
    43441 gatggctgta tcccgcacgg tctgttccac gcgaatcagc tcagacaacg gcaccatgtg
    43501 gccggcagtg gttcgcaacg aaatctcccc gagatgctcc aaaccggtcc gttcgatgac
    43561 cggcaggcgc agtcgcacct gcaccggccg attctctttg ggaatgtgca ccagccccac
    43621 atcggcgcct tccaatgcca gtcgtaacgt ccgcacgatt tcctccgacg gaatcccggc
    43681 cagcgccgcc ttcgcgcgat ccacgctgaa tacatacttc acctggtcgg cttcgaggta
    43741 gtcatcgaca tcgacgacgc cggccgtcgt ctcgaacagt ccgcggacct ctcgcgcaat
    43801 cgccctctgc cgctcgtagt cgggcccata gatctccgcc accagcacag attgcaccgg
    43861 cgggcctggt ggcacctcca gcactttcac attcgctccg aagggccgcc cgatgtcctg
    43921 caccgcggaa cgaatccgtt gcgcgatgtc atgactttgc gtcttccgct gctccttcgc
    43981 gaccagattg atttgaatgt cggcctcgta cggttggtcg cgcagatagt agtgccgcac
    44041 cagtccgttg aaattgaacg gcgaggccgt gccgacatag gcctggt
//
LOCUS       DAORRQ010000037        43341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_55_length_43341_cov_31.932472, whole genome shotgun sequence.
ACCESSION   DAORRQ010000037 DAORRQ010000000
VERSION     DAORRQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 43341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43341
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55_length_43341_cov_31.932472"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(543..1178)
                     /locus_tag="PK224_12585"
     CDS             complement(543..1178)
                     /locus_tag="PK224_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16622.1"
                     /translation="MSRIKALRASIESTIDALEQQAQALEAQLTQSKEQAIQRVEQGK
                     QQLRDVVTSVQADLRRSKDVADHIRAEVQAKLDHLQVQLALGKADARDAFDEQREKIF
                     KALNEFETIADQKLTGMAFDSGRLWEQLVGRSNSLDAEFDALRHRLLAEGRQQLTMVE
                     ATKQELLQKLGVYRDDLKVKRQMVRARADTFELDLREGLEQIKTAFRRLFE"
     gene            complement(1238..1825)
                     /locus_tag="PK224_12590"
     CDS             complement(1238..1825)
                     /locus_tag="PK224_12590"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRB16623.1"
                     /translation="MGKFITIIQGHPDAQTRHFCHALADEYAKGCEDGGHEVRRIEVA
                     RLEFPWLRTKDEFEKGTPPDSIKQAQDAIRWADHLVIFYPLWLGSMPALLKAFLEQVF
                     RPGVAFEYRAQGKMAKKLFTGKSARIVVTMGMPALVYRWFFLAHSLKSLERNILAFCG
                     IKPTRATLIGQVEGMNERQRASWLDEMRGLGDVGK"
     gene            complement(1853..2029)
                     /locus_tag="PK224_12595"
     CDS             complement(1853..2029)
                     /locus_tag="PK224_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16624.1"
                     /translation="MVSVARNIVVLVVFMGGWSVAACAPPTPKVAPSVAPPMIEREPV
                     APVRQQDRSFQPMV"
     gene            2481..2918
                     /locus_tag="PK224_12600"
     CDS             2481..2918
                     /locus_tag="PK224_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB16625.1"
                     /translation="MRATEYFVHACDPKTLIVRQIMEDAVVTVSPASSAMAVAELLSE
                     HNFGSVPVTETDGTLRGLVTEFDLLKAVEQGRDLREVSVSEIMTREVITTTDETPLMN
                     LIHVLQERHLIRLPVVKDQKLIGMVARRDIVFGYVKARANYWP"
     gene            complement(3027..3200)
                     /locus_tag="PK224_12605"
     CDS             complement(3027..3200)
                     /locus_tag="PK224_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16626.1"
                     /translation="MNTPRLDLPPRTPASTSHCLHSRLVDRVLTDKGEETGTLRCLEC
                     DAIIPDPIPASAR"
     gene            complement(3459..4070)
                     /locus_tag="PK224_12610"
     CDS             complement(3459..4070)
                     /locus_tag="PK224_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRB16627.1"
                     /translation="MRPNDRPIVTGLLITYGLFWIGLAIAPVDRQDWFLENLLAVALV
                     AVLVLTYRRFAFSLPAYYLILAFLLLHAIGAHYTYSEVPFGFWLKDALALSRNPFDRL
                     VHFAYGLLLVYPLREVLMRLAGVRGMWVSYLAISGILAQSGFFEVIEAVVAMIVSPEL
                     GSLYLGTQGDEWDAQKDMAAAFLGALLTITGTMFLRRDERLST"
     gene            complement(4111..4749)
                     /locus_tag="PK224_12615"
     CDS             complement(4111..4749)
                     /locus_tag="PK224_12615"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRB16628.1"
                     /translation="MGCNKLISIGLLLWYLAVSVWMAQAPVDPQFWLLASILPALFVV
                     LLIATHRYLPLSHTSHALITAFLTLHTIGVHYTYAEVPIGVWMDQVLHLGRNHFDRIV
                     HFSFGFLLAYPMEELFRLSASIHGWVVYYLPVMTVLGLSGLWEIIESWVARAVHPELG
                     VTYLGSQGDIWDAQKDMAAALYGSLLCMTLLLVVRALRGAESALESEAALSE"
     gene            5137..5865
                     /locus_tag="PK224_12620"
     CDS             5137..5865
                     /locus_tag="PK224_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB16629.1"
                     /translation="MPIPLLDIQGLTFEVDRHSIRDRLDLAVQAGEIHALLGANGSGK
                     TTLAYVLMGCEGYRPSAGRVRLDGTELLPLKMHERARLGLTLAWQEPARFEGVTVREY
                     LSLGKPECDPEPALRQVGLDPDRYLTRRLDKALSGGERHRIELASVLSMKPRLAILDE
                     PAAGIDMLSINHIIDIIRALKEAGGSVLLITHQEEVALIADQASQLCAGRIVFSGGPR
                     EAVDHFRGRTCVRCDGEVCGYVRP"
     gene            5852..6787
                     /locus_tag="PK224_12625"
     CDS             5852..6787
                     /locus_tag="PK224_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRB16630.1"
                     /translation="MSDLEELIRVLPMVGAESSILDDTNIAHVVAHGHHILSHRTVPG
                     LRVNMEETPDAIIGKMIIDAGVTIAQPIHMCFGLAHPTGVQQIKIDVQVNEGAQARVL
                     SHCLFPFAKAAEHRMQAVMTIGPGASLTYTEGHYHGPHGGMQVLPHATIKIGKGARYF
                     SDFSLLSGSVGNLDIDYLVEVEENGLAELNAKIFAHKTDRISLKEAVILRGERSRSLI
                     KTRVVLEGDASAEITGITEAHAKGARGHVDCMEIVQGRAQASAIPIVKVFHPEAKVTH
                     EAAIGSVDKKELETLMARGLTPEQAVELIVSGILR"
     gene            complement(7254..8312)
                     /locus_tag="PK224_12630"
     CDS             complement(7254..8312)
                     /locus_tag="PK224_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB16631.1"
                     /translation="MKLVVTVDVEEDQWGIVPHCHATARNVYRLPTLQKLLNEFGIVP
                     TYLLTYPVVRDPHAVGILREIFAAGECEVGTHCHPWNTPPYEEPLNAYNSMLCNLPAT
                     LQFEKLQRLHEAIQSNFETAPVAFRSGRWGFDAEVARNIIRLGYRIDTSVTPYTSWAQ
                     ASGPDFSRFSPRPSMFTEHLRAERDSNHMLAEIPATIGYLHGDFQACAELVERLNRAP
                     LCGFKLGSLLSRLHLLRKVWLSPEMETPAMMMHLVRQMRSQGYELLNLVFHSSALLGG
                     CGPFVRSQADEHAFMRKLHTFLRLALKDGIGFVALSEAASRSFPLTADSVTMRDSQSF
                     VTSCPAGMAESGSRALAQ"
     gene            complement(8357..9481)
                     /locus_tag="PK224_12635"
     CDS             complement(8357..9481)
                     /locus_tag="PK224_12635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB16632.1"
                     /translation="MKMSAPLTIRAVTSLEEFKGLAQEWEQLQRTVPGHSLFLTWEWL
                     YYWAKHYLGQNQLRILLAFDGHERLVGIAPFYLHATRKLGLIPVRELRFLGSETVCSA
                     YLDIIASERDKRSVCRSLYRYLFNDARREWDILTLSEVPAESSTLDLWNELFDEAGKV
                     GEVIGTTCCPVIHLPGDVDAYRTGLGRSRRYTLQRKTKSLQRAGRIQYSSATSPAEVE
                     AAFESVVALHQQRWSPRPGGGVFSNERAKRFHRDIVGVLSERGRVSIDLLSLDGRPIA
                     GIYGFVYQGVYYFYLPGFAPDVIPKASPGLLLLHHRIEQAICGGERMVDLLQGAQPYK
                     LEWSDDIQRSITLRYYNRSVRTVALKLLESGKQAAKILLR"
     gene            complement(9478..11064)
                     /gene="xrtW"
                     /locus_tag="PK224_12640"
     CDS             complement(9478..11064)
                     /gene="xrtW"
                     /locus_tag="PK224_12640"
                     /inference="COORDINATES: protein motif:HMM:NF038142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase W"
                     /protein_id="HRB16633.1"
                     /translation="MENSLVNPSQTVETANHSRFSAGATVASLSHASILLPAVGLGVL
                     LLLLSAPILGDLWNLWWNRYGFSHGFLVPLVSLYLGWLQWPMLRQIPVEPGIAPGLLW
                     LVVATVLLLASEAAGIMTTGSLAFILVLAGLVLLLCGFAYLKALAFPLGYLIFMTPVL
                     DGLTEPLVWPFQLLTANMAASMLQALGIPVLLENSIHIILPTVTLEVVRECSGAGLLI
                     AVLAVGLPLAYLTLQAWWSRITLVLSSVIIAIVANWVRVAVMGLYAQAGGKDLHGPYH
                     ILQGLFVDWVAFGFLFLGAWLLGKMEHVASMPSLHEEKESSSRCISLGPASNRAWWLA
                     CCTLAAATLALYTLDVGTTELKKDLATFPTVIGDWVIDHQQNSESLVGLPDADESFVR
                     SYRASDGRRVALSVAYTRTQRQGKELVGMGTAPLHEKADATVLRVEEGSVPANRTIID
                     RGHRSIPVTFWYHINGTGYADRNLAKLATIKQAFVRGRTDGALVMVSTEPRKDDSEES
                     WKRQEAFAAAVFPLLQEYLP"
     gene            complement(11385..13556)
                     /locus_tag="PK224_12645"
     CDS             complement(11385..13556)
                     /locus_tag="PK224_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16634.1"
                     /translation="MARCLSCLCFIGFSAFLVSCSEAPSERAAKHESRGDGYVQQEKF
                     LEAVIEYKNAARVAPDNPAIHWKLAKAASQVGDPSTIFTSLSRVVQLDPTDFEAQWSL
                     GDFYLAAGKTDEVAKLAESLLAARPQHPAAYLLRAGLALGGDRVADAIGLLKQASELD
                     PTMVRPLLALANIYFAQQELRQAAEWYDRALKVAPSSADVRIARGHFLFATGIPEEGR
                     KEFQKAVELSPDQEQVRLVLADRYVALGRRDEAEHELAGLIADMNSHKARKALAELKL
                     TAGQVAETKPLVNAILEAEERDPVGIYLKGRIALAENDVPQAVGLFEEAIGKNAALSG
                     PHLYLGLARLAQGRVDSAQEALREAIRLQPDNEAAHLALAKLNLAQQKQAEAEKEAWQ
                     ALRLNPANLEAAVLYGDAFVLGKNWAKAEEVYGAIVRQLPGHPIGYLKMAALRKLQAR
                     PDEAAQLFSQALSRAPDDLIVLQDYLVSLVESKQAQQADRVLDQYLTKAPRDPNLWRL
                     AGRMYVSQGKTDQAEKAFRKAVELAPDVALVHYELGQLYLLERKLPAAESAFQTALKK
                     DENNSEVHTALGVVLASQGRVDAANVHYKRAFQLNPQNVVAANNLAAGLSEQQDLDDA
                     LGFARDALALAPSNPAIKDTLGWVYYKQGRFDKAYPFLAEASAALPQHPVVRYHHAVA
                     LAKIGKQEEALSELKTALSLPGGFPEADRAARMLAANKVEE"
     gene            complement(13608..15896)
                     /locus_tag="PK224_12650"
     CDS             complement(13608..15896)
                     /locus_tag="PK224_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRB16635.1"
                     /translation="MTHVGDGLAVQIEPNPNPDGNTITITPSTPRENLVPFTNVGTIN
                     IEPLASFQNSNQFNNGILLPFTPGGAVINSGTIINSDQFNNYRRVSILEGSRFINLPS
                     AFYYNGGGVTQINGIFINEGAAEHNSNGFAVSETGQYIQRQAVGSSVVPTTVNRDMVF
                     RNAGQVSITAGEFINRPFGIYAQTGTTQIDAVFLNDGRVFNDGRIAVGATGLYEHQSA
                     SPAPPSTQGSLTVNNGTFTNAGTTNINTGVFQNKGGFLSSGIVHIGEGATFTNTSGVY
                     HPFPGGTTKIDGNFINNFAVQNGGAITVTTTGSFSQVAGLSPIGTGTGQGGTFTNAGR
                     VYIGEGTEFRSGVLADRPTSSGQYVQLASGTTRIDGSFDTRGYVTNEGAMTVGATGQY
                     LQSRGDSTGLPAVSTNNIGTFTNAGLVRIGEGTSFSNFPRLEHGSPPPVGGHYIQQSG
                     GTTQIDGSFSSFGGRVTNAGAITVGATGTYGQTRSGAGVGATPATVNTGAFTNAGNTL
                     VNAGTFTNQGSVVNSGTFQVAVNGVATLINHGTVVNSGTFEVGAGVGAVGGVSGTGTY
                     LQNAAAAHTIVNGTFAHNITLQSGALIGTGTIQGTVNNSGGLLLPGNNGLGTLTIKGQ
                     YAQGFGGTLGINLAGLNSYGLLAVHGTGTAVALNGFLTIRLLNGFSPSLGNTFGVLTC
                     TGCTISGGLRGLSLPTLASGLDWSVGFVSGLGTLQLAVVSAPTASAPEPGTLLLVVAG
                     FVALVGWRRKQKKACSAQRAAL"
     gene            complement(16223..16606)
                     /locus_tag="PK224_12655"
     CDS             complement(16223..16606)
                     /locus_tag="PK224_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16636.1"
                     /translation="MKSVRGESRTSRSEKNGATPDPVARKAAAGRREQHKDAVTAAME
                     ESQRRGSAGAAPQVSAALMRAVEAVKRQQKKQGITQEWDLAQRRTETEDPTEFARALG
                     RAVAAAERARKKQVITRAIDKSQRG"
     gene            complement(16728..17396)
                     /locus_tag="PK224_12660"
     CDS             complement(16728..17396)
                     /locus_tag="PK224_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB16637.1"
                     /translation="MVCTDHRYDRCRVLLADDQLIVAAGVRALIEPSCDVVGIVKDGR
                     SLLVEAERLRPDVIVMEVLLPLLNGLDAARQLTKLAPESGLMFLTVQESSRMVADAFK
                     VGASAYLLKRSPASELSQAISAAQRGDYFLTSLLIKDMMVTNQSDATGVSVCTSVSSL
                     TPRQREVLQLIAEGRGTKEVATMLNIAVKTVEFHKFRIMDHLNLHSTVALTRHAIAEG
                     LVSP"
     gene            17753..18190
                     /locus_tag="PK224_12665"
     CDS             17753..18190
                     /locus_tag="PK224_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB16638.1"
                     /translation="MHARRLFYALMLIVMLLSTASGRDAEAADTGTVMKGNAGQGQAL
                     FNGKGICHYCHGVDGVLDKKPSLKPDTAAAIAKLSAGAPDLRNRAGLTLKDNKARFRA
                     IREGHPGSGMLPDTTLSDQDITDLLAYLASLRQSQTTPGKSPY"
     gene            complement(18233..19222)
                     /locus_tag="PK224_12670"
     CDS             complement(18233..19222)
                     /locus_tag="PK224_12670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRB16639.1"
                     /translation="MQIALTGDVMLGRMVDQEVIRNQSLRPEAVWGDVLPILLAADLR
                     VINLECVISARGREWRPQSKAFHFRAHPRAIDCLKSASIDAVTLANNHVLDYGAEALL
                     DCLGLLDRAGITHAGAGRNLATAMEPAFLSSTGSRVAVLALTDNEPDWEAADVKPGVV
                     YVAYARSGLKEPYRARVVEAIRRARRQAGLVLVSAHVGPNWGVPSVEMRALARELLDL
                     GADFYWGHSNHSPQGIELYHGKAILYSSGDFIDDYMVDENERNDLSFLFVLDVEPGRV
                     AHIRLHPVRIEHCRVRLARHQDALFLQKTMQAKCAALGTPIAFHDGVGSVTIG"
     gene            complement(19242..19697)
                     /locus_tag="PK224_12675"
     CDS             complement(19242..19697)
                     /locus_tag="PK224_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRB16640.1"
                     /translation="MDENATGEKEPILWEGGAAWSQFIWLYLIVGIMMLRVALLVRSS
                     LSGWSAWLVGAITLLGTAAALRRWGRYVVTSRRVVLRNGWTGRDIQSIAYREIREISI
                     KQGPLADLMGIGTVVVRGRQDDATIRFPGVFDPEDVKQRIQAALAGRSA"
     gene            19969..20964
                     /locus_tag="PK224_12680"
     CDS             19969..20964
                     /locus_tag="PK224_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB16641.1"
                     /translation="MDQQHPSPSRWAIVLAGGEGTRMQPLVHRWLGFNRPKQYCAFVG
                     SRSMFQHTVDRAALVTAPERIVAVVGRDHRDEILTQLQGRTIGQVIYQPRNCETAAGL
                     FLPLTYIHARAPGATVVIFPSDHFIHPEDRFLAAVRRITDLAETMPDRLVLLGIRPDR
                     LEMEYGWILPGTALSSPEEAPICEVHSFMEKPSRIQAVKAFRHGALWNTFVFAARAEL
                     LWQVGTRCFPDVMRRFERLRSVIGQSQETAVLDAIYEDMPAYNVSTTLLQQAAESAVV
                     VELQDVLWSDWGQPARVAETLRRIGRTPAFPMECLDPPFSTKPLTGADPAPVVQP"
     gene            complement(20982..21443)
                     /locus_tag="PK224_12685"
     CDS             complement(20982..21443)
                     /locus_tag="PK224_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRB16642.1"
                     /translation="MPFELACSAFKEGELIPKKHTCEGEDLSPPLRWNHPPAGTRSFA
                     LIADDPDAPGRTWVHWVLYNIPLDLCGLAEGTPTQETLPNGAQQGLNDSQEIGYGGPC
                     PPPGKPHRYYFKLYALDCELALKSRATKVQVVDAMKGHVLAEASLMGRFSR"
     gene            complement(21701..22636)
                     /locus_tag="PK224_12690"
     CDS             complement(21701..22636)
                     /locus_tag="PK224_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRB16643.1"
                     /translation="MVIPKGRNEKNTIRIGVVGFGKVGRACAELLLTSKDVDLAAIVR
                     RLDSLAQPLPEVFSKIPVVSHTAQVHEMDAALLCVPIDQVEGVAHDCLQHGLPIIECA
                     LLHGEAFQAHREAIDRFATRFDVPAIVGAGWDPGALSVMRSLFGLLAPEGESEMRHRV
                     AASLHHTAMARRVAGVKDALCTEQVAANGTRQRYVYVELEKGGDADRVAAEIRADPLF
                     LGEETLVFPVDSVAALEQEGRGVALERRSAPGRAGHQRFLFEARFDEALLTAHMMLAA
                     ARALPGLSPGVHSLLDLPVSALWGVQAQTAERIWL"
     gene            complement(22630..22857)
                     /locus_tag="PK224_12695"
     CDS             complement(22630..22857)
                     /locus_tag="PK224_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16644.1"
                     /translation="MTLTMPDALRLLELFHQRDYAQARPHLYVKSLKSDSWVRPKGMR
                     VIPSNLDIEILGLESEQWETIPFKDVSFGLW"
     gene            23205..23876
                     /locus_tag="PK224_12700"
     CDS             23205..23876
                     /locus_tag="PK224_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16645.1"
                     /translation="MGPSVVWVLLLAGFLLSAGCIGPLQQWGEETTFRPKATSFAPAT
                     LKHEQVAVLNAVVGFGLEGFAHQVSRSLSSALDQRPTLTTALPVHEALNRINRGELGE
                     EYAAMVADYVRTGILNRAGLQKIGQAIHTNYVFQPSLASFNQSMSGRFSFFGLRVLQT
                     RVTMLRMSLQLWDTRTGEIVWESSGEATLAGEDVREFRIPFDEIARRLWAHMLDDLFK
                     DVPVE"
     gene            24214..24843
                     /locus_tag="PK224_12705"
     CDS             24214..24843
                     /locus_tag="PK224_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB16646.1"
                     /translation="MRLSHFLQVISAGLAFALLLVSPADATLEGAPPTTVVRATLDTV
                     FHILDDQQLKVPGQLSHRRRLLEEVIAERFDYEEMSKRTLAAHWKPLSHTERQEFVQL
                     FKTFLSDQYAARIEGYAGERVVYLSERTVDGYAEVRTRLVSDKLDFPMDYRLTNKSGT
                     WYAYDVIVDGVSLVMNYRSQFGKIIGESSFQELLRRLKERRVSEEKTKT"
     gene            complement(24870..25292)
                     /locus_tag="PK224_12710"
     CDS             complement(24870..25292)
                     /locus_tag="PK224_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16647.1"
                     /translation="MRISYDITRTMARGLLLTCVILLLAGGWSTAAAIENDWLDAMIR
                     AVLVEQAKEGASGRLFALYLGQLTMVRAHLISGESEAVYRAMNRFMEMLQTREQGISA
                     EAADRLFDYCYLVTPARYHDVSRHLNRLSGYQLPTSIG"
     gene            complement(25344..25838)
                     /locus_tag="PK224_12715"
     CDS             complement(25344..25838)
                     /locus_tag="PK224_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HRB16648.1"
                     /translation="MMAVTLKAVPIEKPEYVNLILGQAHFIKTVEDLHEVLVTAVPGI
                     KFGLAFCEASGPCLVRWSGTDEALVAAARTNASAIGAGHSFLVLLKGCFPINVLPAVR
                     QVPEVCTIFCATANDVEVIVAETERGRGILGVVDGASPKGVEGDAEIADRKALLRQFG
                     YKLS"
     gene            complement(25884..26765)
                     /locus_tag="PK224_12720"
     CDS             complement(25884..26765)
                     /locus_tag="PK224_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB16649.1"
                     /translation="MRWLVALDESECSEWIIGWMRAFPHSKQTVVTVVHVLAPLEVPE
                     SIGVSGQQLLLQQQSAMVEALLDRVRRLLEESFADVEVIVREGVPSSEILHVIQERRP
                     DLVVSGMQGLYRSPGFTIGGVAQRLLSYAPCSLMLVPGKVQAGGGLRIMLATDGSEDA
                     QRAACVVAGLPGIREVTIVSVVRPLGAEKAVLERFQPDESRKMEAAFLRQRRAEARKA
                     IAGCERLLLDASITVRARVIAGHPAEAIVRAARRDAVDLLVVGSRGLTGVKAAALGSV
                     SQAVAQLAPCPVLIVKP"
     gene            27871..28368
                     /locus_tag="PK224_12725"
     CDS             27871..28368
                     /locus_tag="PK224_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16650.1"
                     /translation="MLTPVEIAALITATKGAVDIFDKVAGQVKSVLTKRSKEVAGDDD
                     QWRYKIGSDAAATSIIVKQDNRVVQTLKGDELAKMLSPSDLRLIKTYESKMSEYYDLW
                     EKVYEEKDASNDALANAKTEKQLNKLIAKMRDELLGILRFLEQIGVHLDDHYMQIRHL
                     VQDVK"
     gene            28549..31542
                     /locus_tag="PK224_12730"
     CDS             28549..31542
                     /locus_tag="PK224_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16651.1"
                     /translation="MAAKKRIAKMHGEKPTQLARALYIPQKMSHRSLSASVPTDVLAS
                     TAPRVPLPSVLCCPAHLVRRDEFPVVHESPRQISQRSIRVTTSSVTTGEATAPIGEIP
                     SAEVFQQRVQDLLDDGHGTTHTPGGDIAASDAFYDFDEYLFLRNTDDPYVDPRVAGST
                     QVIPADPRMSRFTAAARQGIEFFVDFAVINSSVPYNQISQASIAALEQLLFSWWSSVC
                     KVRAYNELMQYSPTAIRTKLFQQQTNLTPEQYYGSALYARLADLKLAEGKGYFDGNGD
                     SSRLGDLVSADFWERLLTLVFKEHFAGIPTTDSGERGAEYSFDPYPANEVLFGLQVIH
                     RQSWRSLGNGRGELVKSIPLGPRETQKVSVKVTTRTKLTRGSEEASSFETSSESSTST
                     KDTAEVVSEASEKMNKHAEAEVSGGYGPFVQAKVSGGISQDLASSSRQTKNRLNEVMQ
                     KTAGRMKRDTKVTVSTESEESYEVSRSSELVNPNDEVAVTYLYHRLQQRYWVSTRIAE
                     VHSVVFVPERLPEPTEIDENWIARHADTIAGALLDQGFAGILNTIRKEPSTLTSTFST
                     AFQRAADTAISATGNFRNYTGQGTMPDFLASGQRFHERDMERRNAFNMDRARRQHQAN
                     ALIAHICRHILHYMRAIWRAEDYDQRMQRYSRLRVPTRWYFIPRTPQSAGAPSATPLQ
                     VDGFYVADDSSARPLTEVIDPIGPIGYLFNCAIYRLRDDDRLANTHQALAYLRSHYVR
                     FSVQCTPSVGAGVTVRQAVARAPQSFSSDYVLTYRQQRVKWLLPIPNRSEGDWFEASK
                     LEDGSIEILGIRIWIDGTPADRAELKIAVRVTSELEDPQVRHTMLQYPLPLHSNEPGF
                     FTASILATMADLLPPVATALAGTHDWAQLTEAQRTVVRAYYHEWIVLRDSGRLVPIET
                     SNVLLDLEVGPSPILEPFKRLHRYIDVMREYEIMRKNHLENGRREALLQAGRLGDPEI
                     ERVSVVSSDPSLAKLVTVTDDTPGN"
     gene            31561..31866
                     /locus_tag="PK224_12735"
     CDS             31561..31866
                     /locus_tag="PK224_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16652.1"
                     /translation="MATKRTTAKPSASRKLDSARLRDLLTTVPRFSKAKLNDEVARAL
                     RFLETRPELKARLDSGALTAVQLLAMVRAQAEVRMGESSDQHTRREVDRYLGSDRTG"
     gene            31901..34375
                     /locus_tag="PK224_12740"
     CDS             31901..34375
                     /locus_tag="PK224_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16653.1"
                     /translation="MAEPTLEEAATRLGSVYAELVYAGHALDEQTLPNWIEPERLRPV
                     GGFDIGTKLLLALRKSPLYFTQLGDAVRREAGYPNLQFDNLRASRFQYERDLRVFHNR
                     LKPALAKYTQGAMIRQEQELRKLRGHFGKPGIEDELHTSFQRIWFAVEEVLPGGVRPR
                     SGENESTAAGADNAPASSDAGETSSENLPGGVAGPVDPEAGQLNTNPDDMPTDFVERY
                     QMLRAIKVERERLQSETKEGGNALNNEIADLLATIAVHPEVVNIARHYVWSPIKGTEY
                     TQTAVDQAIAAVADLRERLTQREPDDIWNYAPLVVGTLTQLKLHEFPAVPEFSVALGA
                     MLGRTKTNVYLGAATILIVGLSLVFTGPVSAVVVGSLDLALAGTTLGITRLRAHEQEI
                     AATASDFSPDDQKLAAHTTSTEGALDAAGALLSAITLVGSVRQLTKVRELLRIESKGL
                     KVIRSSKRELDTADRAGGFGNKVKDASGRDATSYELEDQINSTLGNPNALTEEVMQRR
                     AERTGTRTLVGEKWEYTPDKMRRQGAKPGPNDPEFGLGPQPDFELDLGDGIELELADW
                     RKTGAAESPVHKRPDFHEIEPKSLESLPAVTHKQNQLSGVREITEAEVTAAQTASRQP
                     PDLEALYVRERVTTREQITGLYERDPFYPKFEEQKAMLIQSGAKDPIIPNYKFSKKNP
                     PHVDHIIPENQVRKFEGFTELDIEFQREVLHMDKNLELVSSSVNLSRGDMPYSLWDGT
                     MEFKIPPQIRKAKIAQEKALAIEIQNKIYNLLERQRQLRGTTRPSAMPIKAGARLTGD
                     VSRGLRLVNERETKQHKSGSLDPKKW"
     gene            complement(34625..35971)
                     /locus_tag="PK224_12745"
     CDS             complement(34625..35971)
                     /locus_tag="PK224_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRB16654.1"
                     /translation="MMTDKAELLAKASKPAEEALRLHAYYKGKMQTLPKCAIRSLEDF
                     SVWYTPGVAAPCKAIQADHELVYDYTNKGNTIAIVSDGTRVLGLGDIGPEAGLPVMEG
                     KALLFKYLGGVDAVPICLDTNDPDELIRTVRLLTPSFGAINLEDIAMPKCFRILRELQ
                     ADCPIPVWHDDQQGTGTVLLAGLINALKVVGKEIGQVRIAMIGMGAANVPTYRFLKVF
                     GADPARIVACDAGGILGTHRREYEQDPAFSEQWKVCLHTNPTGLRGGIAEALQGADVC
                     VAFSAGGIIKPEWVKGMARDAIVFACANPVPEIWPWDAKEVGARIVATGRSDFPNQVN
                     NSLVFPGMFRGLLDVRARAITDEMAIAAAHELALCARERGIHEGSILPTMDEWHVPMR
                     LAVATAIKAQEQGLARVTRTRDQVHILAESKIRSAHEAMRVLLREGLIVAPAAARE"
     gene            complement(36036..37766)
                     /gene="polX"
                     /locus_tag="PK224_12750"
     CDS             complement(36036..37766)
                     /gene="polX"
                     /locus_tag="PK224_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HRB16655.1"
                     /translation="MTVHNAEVATAFEEMADLLEIEGANPFRVRAYRFAARTLRDLPA
                     EVGEMVAKGDDLTSLPGIGDDLAGKIKEILATGTAAAIEAQRKRVPATLTELLRIPGL
                     GPKRVKRLAHELKIRSLSELQTAAQAGRVRTLAGFGEKTEQHILDALATRLAEAPRVQ
                     RAVAIPSAEALVAYLEQSSGVSRVIAAGSYRRGLETIGDLDILVTAPAGRAVMDRFVA
                     YQEVRDVLARGETKSSVRLQSGLQVDLRVVPQDSYGAALLYFTGSKAHNVVLRQLAQQ
                     RGLKLNEYGVFRGDKPIAGDTEESVYASLGLPWIPPELREGRGEIDAAKAGRLPHLVD
                     LQDLKGDLHAHTKATDGRHSLQEMAEAARLRGLRYFAITDHSRRLTMAKGLDSARLLQ
                     QTEAIDRLNATLSGITILKGIEVDILEDGNLDLPDEVLGRLDLVVGAVHSRFNLSNRR
                     QTERIMKAMDHPHFSILAHPSGRLIGRREPYDVDMLRIIRKARERRCFLEINAHPERL
                     DLTDIHCRMAKEEGVLLAVNTDAHSMLDLENARFGIGQARRGWLEKTDVLNTRPYAEL
                     RKLLKPTMEG"
     gene            38179..39069
                     /locus_tag="PK224_12755"
     CDS             38179..39069
                     /locus_tag="PK224_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRB16656.1"
                     /translation="MAGWIDRWRAATVAIGTIQDADVRSRTGRVSTKKFFAVVGTGVL
                     FSLHGPRRQDCWLVTARHVFLDPTENWKPSTLGIVFPHIHRRGVKQGVEIPIQLTKAG
                     RRCWYPHPDKAVDLACLPLPLTMRQPKPGGPSSIAWMDIATTADLYEGVPIMVLGYPA
                     AFDIPDSPRAIVRQGIVSWVSPARPGSEVFLIDSHVFPGNSGGPVFRLPTAMDREGHL
                     TAGGAVTLLGIVTQARIQSLPLLAGGKQVDLYVEGKKNSEPLLTPSFLGLGLVEPAYR
                     IKQLLVSATRRHRRRKRPVS"
     gene            complement(39083..39301)
                     /locus_tag="PK224_12760"
     CDS             complement(39083..39301)
                     /locus_tag="PK224_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16657.1"
                     /translation="MVTHPTQIAQDRCHRCGGLMVREEIREVNQIGMRCVMCGEHIDP
                     LILEHRRKMRTPEGVRQLLAKGAKAGMN"
     gene            complement(39382..40728)
                     /locus_tag="PK224_12765"
     CDS             complement(39382..40728)
                     /locus_tag="PK224_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HRB16658.1"
                     /translation="MDNRAKPHEIRVLIMGAAGRDFHNFNVLFRDNPDYRVVGFTAAQ
                     IPNIDDRLYPPALAGALYPSGIPIHAEQDLDRLIRTQHVERVVFSYSDVSHEALMHQA
                     SLVMAAGADFWLAGPQSTMLPAKKPVVSICATRTGAGKSPVARRVVSILKGEGLRVAA
                     VRHPMPYGNLEQQAVQRFAALEDLDAAACTIEEREEYEPHLTQGGTVYAGVDYERILR
                     EVEEQADVIVWDGGNNDWPFFMPDLELVLVDPHRAGEPPYFPGEVNLIRADVVVLTKL
                     DTATPEQVAAARRSIETVNPRAALVETAMPPKVDHPDRITGRRVLVVEDGPTLTHGGM
                     AFGAGCLVARRYEAGCLVDPRPYAAGSLNRTFQQYPHLGPLLPAMGYGPEQIRELEET
                     IERVECDLVVIATPMDLRRLIRITKPTVRVSYDVEDRGRPTLADVLQGIIGRAKQS"
     gene            40905..42665
                     /gene="acsA"
                     /locus_tag="PK224_12770"
     CDS             40905..42665
                     /gene="acsA"
                     /locus_tag="PK224_12770"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRB16659.1"
                     /translation="MPWNPIIKSRRDWETIPNLHDYESVRKDFSWEQARAELDGLPDG
                     QGLNIAHEAVVRHATGPLGQQTAIRWLGKSGQVEDYSYARLDDLTNRFANTLQGLGVV
                     KGDRVFVLAGRIPELYITALGTLKHRALFCPLFSAFGPEPIRARLTIGQAKVLVTTES
                     LYQRKVAGIRETLPHLEHVLLIGEEGRPTAIPGTQNFHQLLEQHPGIYRIGPTDPEDA
                     ALLHFTSGTTGTPKGALHVHGAVVAHHITGKLALDFHPADIFWCTADPGWVTGTSYGI
                     IAPLTNGLTCIVDEADFEAERWYGILQDQRVSVWYTAPTAIRMMMKVGVDLVRKYDLR
                     QLRFLASVGEPLNPEAVVWGEKAFGRPFHDNWWQTETGGIMIANYAAMDVRPGSMGRP
                     LPGIEAGIVKKTESGGLEVLNEPDVQGELALRPGWPSMFRGYWNEPERYKKCFAGGWY
                     LTGDVAKKDADGYFWFVGRADDVIKTSGHLIGPFEVESVLIEHKAVAEAAVIGKPDPV
                     AMEIVKAFVSLKDGHEPSEALRRELLGFARARLGAAVAPKEIAFLPTLPKTRSGKIMR
                     RLLKARELGLPEGDTSTLEA"
     gene            42753..>43341
                     /locus_tag="PK224_12775"
     CDS             42753..>43341
                     /locus_tag="PK224_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRB16660.1"
                     /translation="MEFKDYYKTLGVERTATADEIKSAYRKLARKHHPDVNKETGAEA
                     RFKEIGEAYEVLKDTEKRAAYDELGNRWREGQEFTPPPEWGAGFEFTQGGASATEAAD
                     FSDFFSSLFGNFSRQAESTHARGEDHHAKIFIPLEEAFHGGTQTLTLRAPQVNAQGRP
                     ELRERSLTVHIPKGVREGQHIRLAGQGAPGIKGTSP"
BASE COUNT         9299 a        13557 c        12147 g         8338 t
ORIGIN      
        1 ggccatgatg tcgtcctttc gatgattgaa aaaatgaaaa gaggctacgc ggctgaagaa
       61 gccgtgtcaa ctgaactcgg cccagcgcag gaggcgtatc tcttcttcac agaccgtatc
      121 gagagagaga cagatccgcc agggggtcat gcttcgtacg tctcgcgaac ataacaataa
      181 ccgtcactct ccatccccga gcatgaggca agatctttgc ggacgccgtc tcccacctgg
      241 gcatcatgga gatccaacac ggtatgcacg ttgaacgacc cgaccgtccg acagcctggc
      301 agcagcccga cgcacagcac gagtatcaac aatccgagca ccaagccgtg ccgcgcgtcc
      361 gtccggcaca atcaggatga acaatagtca cctcagccac ctctcaagat cgttcgcttc
      421 acagggcagg agcggccgca ccgcgacggc cgcctgatac atcacgcgcc acggtatctt
      481 gatcggaagg cggacaggtt caaacccgga aggaaggaga gaagggtcat aaagagtgtg
      541 gattactcga acaacctgcg aaatgctgtc ttgatctgct ccagcccttc ccgcaagtcg
      601 agctcaaacg tatcggccct ggctcgtacc atctgacgct tgaccttgag atcgtctcga
      661 tagacgccca atttctgtag cagctcctgt ttggttgcct caaccatcgt gagctgctgc
      721 ctgccctcag caagcaagcg gtgcctgagg gcatcgaact ccgcgtcgag gctgttcgac
      781 cgtcccacaa gctgttccca caaccgcccc gagtcgaatg ccattcccgt caatttctga
      841 tcggcaatgg tctcaaactc gttcagggct ttgaaaatct tctctcgctg ttcgtcaaac
      901 gcatcacgag catcggcctt gcccagcgcc aactgcacct gaaggtggtc cagcttggcc
      961 tgtacctccg cccttatatg atcggccacg tccttcgacc gcctcagatc ggcctgcacc
     1021 gacgtgacta cgtcgcgcaa ttgctgtttg ccctgctcca cccgctgaat cgcctgttcc
     1081 ttgctctgcg tgagctgcgc ttccagcgcc tgcgcctgct gttccaacgc atcgattgtg
     1141 ctctcgatcg acgctctgag tgctttgatc cgcgacatgc ggcttcctcc ctcccgccta
     1201 gacacagatc cgaacataga agatcaacgg ggtaagctca cttccctaca tcgcccaagc
     1261 ccctcatctc gtccaaccag ctcgcccgct gccgctcatt catgccctcc acctgaccga
     1321 tcaaggtggc cctcgtcggc ttgatcccgc agaaagcgag gatgtttcgt tcgagacttt
     1381 tcaggctgtg ggccagaaag aaccatcgat acaccaacgc cggcatgccc atagtcacca
     1441 cgatgcgagc cgacttcccc gtgaagagtt tcttcgccat cttcccctgc gctcgatact
     1501 caaaggccac gccgggacga aagacctgtt caagaaacgc tttgaggagc gccggcatcg
     1561 accccagcca gaggggatag aagatgacga gatgatccgc ccaccggatg gcatcctgag
     1621 cctgcttgat cgaatcaggc ggcgtaccct tttcaaactc gtctttcgtc cgtaaccaag
     1681 ggaactccag ccgggccacc tcgatacgtc gcacttcatg cccgccgtct tcgcagccct
     1741 tggcatactc gtccgccaag gcatggcaaa aatgtcgagt ctgcgcatcg ggatggccct
     1801 gaatgatggt gataaattta cccatagcac ctcccccatg ccctacgcct ccttacacca
     1861 tcggctggaa tgaccggtcc tgctggcgaa ccggtgcgac aggttcccgc tcgatcattg
     1921 gtggcgcaac cgatggggcg actttcggag tcggcggggc acaggcagcc accgaccaac
     1981 ctcccataaa cacgactaaa acaacgatgt tccgtgcgac actcaccatg tcctccaaca
     2041 tgcgtcggtg tgctgtcggg acccggcctc catcaggcgc aacaggcgtg ccaacattcc
     2101 tctagtcacg atcggcacaa cacaggggct tcagaggtcg acggaggctt cagggccgaa
     2161 taggactgcg gagaatttcc gcagcaccgg tgccgggccc tgtcgcaaat atccacaatg
     2221 cctgcctgca aacaaagacg ggaacgagtg ccctcccgcc ctggtatcgc gcaccatccg
     2281 cgcatttccg acaggagcgc catgctttca aacggttcgc ttagagcccc caagatccaa
     2341 gcggttgact cctctcccac cttgatggtt ttgaagagac cggcagtggc accaagcttg
     2401 ctcctcttcg aaaggggaaa tttcacctga aacatagagt cctgtcacct cactccacgg
     2461 tggaacggaa ggagggtcct atgagagcca cagaatattt cgtccacgcc tgcgacccca
     2521 aaacactgat cgtccgtcag atcatggagg atgccgtcgt tacggtcagc ccggcctcca
     2581 gcgccatggc cgttgcagaa ctcttgagcg agcacaattt cgggagtgtc ccggtcaccg
     2641 agacagatgg caccttgcga ggcctggtca cggagttcga tctgctgaaa gccgtcgagc
     2701 aggggaggga tcttcgcgaa gtatcagtct cggagatcat gacccgcgag gtcatcacca
     2761 ccaccgacga gacgccgctg atgaacctga ttcatgtcct ccaggaacgg cacctgatcc
     2821 gtctccccgt cgtaaaggac cagaagctca tcggcatggt cgcgcggcgg gatatcgttt
     2881 tcggctacgt caaagcacgc gccaattatt ggccgtaatg agagacagac cggcggatat
     2941 ccttgagcag ggccgggaca cggccggaag tcgcgcagaa tgagagggag tgccgattcc
     3001 ctgtcgatga agccagcgag gggaaattac cgcgccgatg caggaatcgg atcgggaatg
     3061 atcgcgtcgc actccaaaca gcggagggta ccggtttctt cgcctttgtc cgtgaggacg
     3121 cgatcaacga ggcggctatg gaggcagtgg gaggtggatg cgggagtcct gggcggcaga
     3181 tcgagtcgtg gagtattcat ggcgccctcc gtcgtaagcc tgagcatcat ctctgaccca
     3241 aatccgctcc ggatcatagc agaatccttc gagtgattcc gttaacctgg actattcatg
     3301 aataccgtcg cgagacgttc gagacagaag cccgccgagg ctgctcgcac gtgaggccga
     3361 cgcaggcgta ctggtagtcc gtcgaggagg ccgaacgaga acagcctcgc cgggcgacag
     3421 aatcgaacgc cggagcaggt attcatgaat agtccgggtt aggtagagag ccgttcatcc
     3481 cgacggagga acatcgtgcc ggtgatggtc aggagcgcac ccaaaaaagc ggccgccata
     3541 tccttctgcg catcccattc gtcgccttga gtccccagat acagacttcc gagttcaggg
     3601 ctgacaatca tcgcgacgac cgcttcaatc acttcgaaaa aaccgctctg cgccagaata
     3661 ccgctgatcg ccagataact cacccacatc ccccgcacgc ccgctagacg catgagcact
     3721 tcgcgcagag gatagaccag gagcaaccca taggcaaaat gcacgaggcg atcgaacggg
     3781 tttcgactca acgccagcgc atctttcagc caaaacccga agggaacttc agaataggtg
     3841 taatgggcgc cgattgcatg gagcagcaga aacgccagga tcagataata ggcagggagg
     3901 gaaaacgcga accgccgata ggtcaggacg agcaccgcca cgagcgcgac tgcgagcaga
     3961 ttctccagga accaatcctg ccgatccacc ggcgcaatcg ccagaccgat ccagaacagc
     4021 ccataggtga tgagcaaccc cgtcacgatc gggcgatcgt tcggtctcac tccgctctgg
     4081 tccctcgccc tcacacccat gccatgccgt ctactcggaa agcgccgctt cggattccag
     4141 cgccgactcc gcccctcgca aggcgcgcac caccaggagc agcgtcatgc acaacaacga
     4201 gccgtagagt gcagccgcca tgtccttctg cgcatcccag atatccccct gcgagcctaa
     4261 atacgtcacc cccaattccg gatgcacggc gcgcgccacc cacgattcaa tgatttccca
     4321 gagtccgctc agtccaagga ccgtcatcac gggaaggtag tacaccaccc acccatggat
     4381 actcgcgctc agacgaaaca gctcctccat cggataggcc agcaaaaacc cgaagctgaa
     4441 gtgcacgatc cggtcgaagt gattccgccc gagatggagc acctggtcca tccacacccc
     4501 gatcggcacc tcggcatagg tataatgaac gccgatcgtg tggagcgtga gaaacgccgt
     4561 aatgagcgca tgggaggtat gggacaaggg aaggtagcga tgggtcgcaa tcaatagcac
     4621 gacgaacaac gccggcagaa tactcgccag caaccagaat tgcggatcca ccggcgcctg
     4681 cgccatccag acagacacag ccagatacca cagaagcagc ccgatcgaaa tcagtttgtt
     4741 acaacccacc gttgtttctc ccgataagtg gtgtgagaag agggggctcc ggccacaggc
     4801 atggcggagc agaccctttc actctactgg atgactcccc ttcctgcaac cgccctgccc
     4861 agccgtgagt ccaccggacg tgatccgtga acctcgcctc gccgccggcg aaaagctgga
     4921 accggcgcgc accagccact cgccccatcc gttccatgac acacacgagc gcgatccatg
     4981 tgctcacgcg gtgaggagta actccccagc agacctggcc gccctgccgc aaaaggcaac
     5041 gttcaacagc ctgacgacaa ccgggaggcc atcgtttcgc agccacacca cgggcacagc
     5101 tgatgcactt cgatcccgct caatggctgc gctgctatgc ccattccctt gctagacatt
     5161 caggggttga cctttgaggt cgatcgtcat tcgattcgcg atcggctcga cctcgcggtc
     5221 caagccgggg aaatccatgc cctgctgggc gccaacggtt ccggcaagac caccctcgcc
     5281 tatgtcctga tgggttgcga agggtataga ccaagtgccg ggcgggtacg attggacggg
     5341 acggagcttc tgcccctcaa gatgcatgaa cgcgcccggt tgggactgac gctcgcctgg
     5401 caggagccgg cccgattcga aggcgtgacg gtgcgcgaat atctcagcct gggcaaaccg
     5461 gaatgcgatc ccgaaccggc actgaggcag gtggggctcg atccggaccg ttacctgaca
     5521 cgtcgcctgg acaaggcact gagcggaggc gaacgccata ggatcgagct cgcgtccgtc
     5581 ctctctatga aacccagact ggccatcctc gatgagccgg cggcgggcat cgatatgctc
     5641 tcgatcaatc acatcatcga catcatccgc gcgctgaagg aagccggcgg atcggtcctc
     5701 ttgatcacgc atcaggagga ggtggccctc attgccgatc aagcgtctca actctgcgcc
     5761 ggacgcattg tcttttccgg cggtccgcgc gaggccgtcg atcatttccg cgggcgaacc
     5821 tgtgtgcgat gcgacgggga ggtctgtggc tatgtccgac cttgaggaac tgatccgcgt
     5881 cctgccgatg gtgggagccg agtcatcgat cctcgacgat accaacattg cgcatgtcgt
     5941 ggcccacggg catcacatcc tcagtcatcg gacggtccca ggcctccgcg tcaacatgga
     6001 ggaaacaccg gacgccatca ttggaaagat gatcattgac gcgggcgtca ccatcgctca
     6061 accgatccac atgtgtttcg gcctggccca ccccaccgga gtgcaacaga tcaagatcga
     6121 tgtgcaggtg aacgaagggg cgcaggcgcg agtgctctcg cattgcctct ttcccttcgc
     6181 aaaagcggcc gaacatcgca tgcaagctgt gatgacgatc ggccccggcg cgtcgctgac
     6241 ctataccgaa ggccactacc acggccccca cggcggcatg caggtcctgc cccatgccac
     6301 gatcaagatc ggcaaaggcg cccgctattt ttcagacttc tcgttgttgt ccggctcagt
     6361 ggggaatctc gacatcgatt atctcgtcga agtggaggaa aacggccttg ccgaactcaa
     6421 cgcgaaaatc ttcgcgcaca agaccgatcg catctcgctg aaggaagcgg tgatccttcg
     6481 cggcgaacga tcacggagcc tcatcaaaac acgcgtggtg ctggagggcg acgcctcggc
     6541 cgagattacc ggcatcacgg aagcccatgc caaaggggcg cgcggccacg tggattgcat
     6601 ggaaatcgtg cagggccggg cccaagccag cgcgatcccg atcgtgaagg tctttcaccc
     6661 ggaggcaaaa gtcacgcacg aagcagccat tgggagcgtg gacaagaagg aattggaaac
     6721 gctgatggcc cgcgggctga ctcctgagca ggcagtcgaa ctgatcgtga gcggaattct
     6781 tcgttaggcg gaggcctgcg caaactgcct gaccggcagg ttcacgagga cgccttcgcg
     6841 gcagtatggg gatcgtgaat gacggctccg cattcgaggc atcgatagag aggacgttct
     6901 gtttcatcct cgcagcgaat acgatcgatg agtcgcatgt ggagacaggt gagtgtccgt
     6961 gtcgtggtgg gctcgagttg aggactacgc atagtgacct ccccgaatcg cagcggacaa
     7021 tagctgggct atttctaacc gaatgcggcc caaccctatc agatctgtgc agacttcaca
     7081 ctaggaattt gtcagatacg atcaggggaa aggtccgaca caacacttgg ggctcaccca
     7141 gtccgtaccg ctacatatag ccgtcatcac cggataaaga atcctccgtt cagcctgact
     7201 cgaaccacag caaacttttg ttggaacctg ttgagatagg cccgctcagg cgatcattgt
     7261 gccagcgctc gcgaaccaga ctcggccatg cccgcaggac agctggtcac aaacgattgc
     7321 gagtcccgca tggtcaccga atcagccgtg agcgggaagg agcgactggc ggcttcggaa
     7381 agagcgacaa acccgatgcc gtccttcaat gcgaggcgaa gaaacgtgtg cagctttctc
     7441 atgaatgcat gctcgtctgc ttgagagcga acgaacggtc cacatcctcc cagcagcgcc
     7501 gaggaatgga acacaagatt cagcaactca tagccttgac tgcgcatctg acggacaagg
     7561 tgcatcatca tggcaggagt ctccatttcc ggagagagcc acactttccg caagaggtgc
     7621 aggcgcgaca agagactgcc gagtttgaac ccgcacagcg gggcacgatt gagccgctct
     7681 accaattcgg cgcaggcttg gaagtcacca tggaggtaac cgatcgtggc cggaatttca
     7741 gcaagcatgt ggttcgagtc tcgctcagcg cgaagatgct ccgtgaacat ggaaggcctt
     7801 ggggagaagc gcgaaaagtc aggaccggac gcctgtgccc aggatgtata tggggtaacg
     7861 gatgtgtcga tacgatatcc aagccggatg atgttcctgg ccacttccgc atcgaatccc
     7921 caccgtccgc ttcgaaaggc aacgggagcc gtctcaaagt tgctctgaat ggcttcatgc
     7981 aaccgttgca gcttttcgaa ttgaagagtc gccggaaggt tgcagagcat gctgttgtac
     8041 gcattgagag gctcctcata cggcggggta ttccagggat gacaatgggt cccgacctcg
     8101 cactcgccgg cagcaaagat ttcccggagg ataccgaccg cgtgtgggtc ccgcaccacc
     8161 ggataggtca gaagatacgt cgggacaatg ccgaactcat tgagcagctt ctgcaacgtc
     8221 ggaaggcggt acacattccg ggcggtcgca tggcaatgcg gaacaatccc ccattgatcc
     8281 tcttctacat ccaccgtgac aaccagcttc acgaccgacg cctccttgcg actcgctcgt
     8341 acgaccgacc ctcgttctac ctcagcaata tcttggcagc ctgctttccg ctctccagca
     8401 gcttgagagc cacagttcga acggaccggt tgtaatagcg cagcgtgatt gacctttgga
     8461 tatcgtcaga ccattcgagt ttgtacggtt gtgcgccctg caagagatct accatccgct
     8521 caccaccaca gattgcctgc tcgatacgat gatgcaacag gagcaggccc ggactggctt
     8581 tcggaatcac gtccggagcg aaccccggta aataaaaata atagacgcct tgatagacaa
     8641 atccgtagat acctgcgatg ggccgaccat ccagcgacaa cagatcgata ctgactcgac
     8701 cgcgctcact caagaccccc acgatgtccc ggtgaaaccg tttcgcacgc tcattagaaa
     8761 atacgccacc gcccggtcga ggactccatc gttgttggtg gagggcaacc accgactcga
     8821 acgccgcttc aacctcggca ggactcgtgg cgctcgagta ctgtattcgt ccagcacgct
     8881 gaagggactt ggtctttcgt tgcagcgtat accgcctgct tcgccccagg ccggttcgat
     8941 aggcatcgac atctcccggc agatggatca cggggcagca ggtcgtgccg atgacctctc
     9001 cgaccttgcc cgcctcgtcg aataactcgt tccacagatc gagcgtggaa gactcagcag
     9061 ggacctcgga gagggtgagg atgtcccact cgcgacgcgc gtcgttaaag aggtatcggt
     9121 acaggctccg gcacacagac cgcttatctc gctcgcttgc aatgatgtcg aggtaggcgg
     9181 aacagacggt ttcgctcccg aggaagcgca attcccggac tgggatcaag ccgagtttcc
     9241 tcgttgcatg gaggtagaac ggcgcaatgc cgaccagacg ttcatggccg tcgaacgcca
     9301 ggagaatcct cagctggttc tgtcccagat aatgcttcgc ccagtagtac aaccactccc
     9361 aggtgagaaa gagactatgc cctggcacgg tgcgctgcag ctgctcccat tcctgggcca
     9421 accccttgaa ttcctctagg gaagtgactg ctcggatggt gagcggggcg ctcatcttca
     9481 tgggagatac tcttgcagga gagggaacac agcggcggca aacgcctcct gcctcttcca
     9541 ggactcttcg ctatcatctt ttcgaggctc agtagacacc ataacgaggg cgccatcggt
     9601 acgtccgcgt acaaaggcct gtttgatggt cgccagcttg gccagattcc ggtcggcgta
     9661 gcccgtcccg ttgatgtgat accagaacgt aaccgggatg gatcggtggc cccggtctat
     9721 gatggtacga ttcgccggga ccgatccttc ctcgacccgc aaaaccgtgg cgtcggcctt
     9781 ctcatgaagc ggggcagttc ccattcccac gagttctttt ccctggcgtt gggtccttgt
     9841 atacgcaaca gagagcgcca ctcgacggcc gtcagatgca cgatagctcc ggacaaatga
     9901 ttcatccgca tccggcaagc caacgagtga ttcgctattc tgctgatggt caatgaccca
     9961 atcgccgatg acggtcggga acgtggccag atctttcttc agttccgtcg ttccgacgtc
    10021 aagcgtatac agagcaagcg tcgctgccgc aagcgtgcag cacgccagcc accaggcgcg
    10081 attcgacgct gggccaagag agatgcatcg gctggaggat tccttctcct catgtaagga
    10141 gggcattgac gcaacatgct ccatcttgcc cagcagccag gcccccagga atagaaaccc
    10201 gaatgcaacc caatcgacaa acagtccctg cagaatatgg tagggcccgt gaagatcctt
    10261 acctcccgct tgagcgtaaa ggcccatgac cgccacccgg acccaattgg ccacaatcgc
    10321 gattatgacc gacgacagca ccagggtgat ccggctccac caagcctgca gggtgagata
    10381 ggccagcggt aacccaacgg ccagaacggc aatgagcaat ccggccccgc tacattcgcg
    10441 caccacctcc agcgtcacgg tgggcaggat gatgtggatg ctgttctcca gcagaacggg
    10501 aatgccgagc gcctgcaaca tcgaggcagc catgttcgcg gtcaggagct gaaagggcca
    10561 gaccagtggt tcagtcagac catcgaggac gggcgtcatg aagatcagat agccgagcgg
    10621 aaacgccagc gctttcagat aggcaaaccc gcagagaagc agcacaagcc cagcgaggac
    10681 tagtatgaag gccaggctcc cggtggtcat gatgccggct gcttcgctcg ccaagagcaa
    10741 caccgtcgcg accaccagcc agagcaatcc cggcgctatc cccggttcaa cggggatctg
    10801 cctcaacatg ggccattgca gccagccgag atacagactg accaggggta ccagaaaccc
    10861 atgtgaaaat ccgtatcggt tccaccacag gttccagagg tcgcctaaaa taggcgcgga
    10921 gagcaggagg agaagaacgc cgagcccaac cgccgggagc aggatggaag catggctcag
    10981 actggccact gtcgcaccag cggagaagcg cgagtgattt gcggtctcca ccgtttgaga
    11041 cggattaacg agagagtttt ccaagcgcca acctcaccga acagcactgc tgcacagttc
    11101 acggaccagt aggtcattga cactcgctga tgctctccga ccaatccgca ggatgttcaa
    11161 aaaggccgtt cagcgaggcc gcagcgaggt gcacggcgcg atgaataaag agcgccacgt
    11221 ctgtgcacgc cggcgagtca gtgagccggc cgtgtcctgg aggcgacgcc acatcattct
    11281 taccgtccac ccctagcctg ccgagacagg cttctttccc gtggccgtac gttgaggagc
    11341 tgaacgatgc gagaacaaag ctggcggact ttttcaacat cctgctactc ttcgaccttg
    11401 ttcgccgcca gcatccgcgc cgcacgatcg gcttccggga aaccgcccgg gagtgacagg
    11461 gcagttttca gctcggacag ggcctcctcc tgcttcccga tcttagcaag ggccaccgcg
    11521 tggtggtaac gaacgaccgg atgttgtgga agagccgccg aagcttctgc gaggaacgga
    11581 taggccttgt cgaaccggcc ttgcttgtaa tacacccatc cgagcgtatc tttgatcgcc
    11641 gggttagaag gagccaacgc taacgcatcg cgcgcaaacc cgagcgcgtc gtccaagtcc
    11701 tgttgttcgc tgaggccggc cgcaagatta ttggcagcga caacattttg agggttgagc
    11761 tggaaggccc gcttatagtg cacattcgcc gcatcaaccc gcccctgcga agccaacacc
    11821 acgcccaacg ccgtatggac ctccgaattg ttctcgtcct tcttgagagc agtctgaaac
    11881 gccgactcag ccgcaggcag cttacgctcc aagagataca attggccaag ctcatagtgg
    11941 accagcgcaa catccggcgc gagttcgacc gccttccgga aggccttctc tgcttgatcg
    12001 gtcttcccct gagagacgta catccgaccc gccagtcgcc agagattcgg atcacgcggg
    12061 gctttggtta gatactgatc gagcacgcga tctgcctgtt gagcctgctt cgattccaca
    12121 agtgagacca ggtagtcttg cagaacaatc agatcatcag gcgcgcgcga aagagcttga
    12181 gagaagagct gcgcggcctc gtctggcctt gcttgcagct tgcggagcgc tgccatcttc
    12241 agataaccaa tcggatggcc aggaagctgc cgaacgatcg cgccatacac ctcttcggcc
    12301 ttggcccagt ttttccccaa gacaaacgca tcgccgtata gcacggcagc ttccaggttg
    12361 gcggggttca atcgcaacgc ctgccacgcc tctttttccg cctcagcctg cttctgttgc
    12421 gcaagattca gttttgcgag agcgagatgt gcggcctcgt tatcgggttg aagccggatt
    12481 gcctcgcgga gtgcttcttg tgcagaatcc acccggccct gcgccaaacg ggccagcccc
    12541 aaatagagat gagggccgga aagagccgca ttcttgccaa tggcctcttc gaacaggccg
    12601 accgcttggg gcacatcatt ctccgctagc gcgatacgcc ccttgagata gatgccgact
    12661 gggtcacgct cctcggcttc gagaatggcg ttcaccaacg gtttcgtctc ggccacctga
    12721 ccggcggtga gtttcagctc cgcgagcgcc ttgcgcgcct tgtgggagtt catgtcggca
    12781 atgagaccgg ccaattcgtg ctcggcctcg tctcgccggc cgagcgcgac ataccgatcc
    12841 gcgagaacga gccgaacctg ttcctgatcc ggactcagct cgaccgcctt ttggaactcc
    12901 ttgcgcccct cctcaggaat tcctgtcgca aagaggaaat gtccgcgtgc aatgcgcaca
    12961 tccgcagaac taggggccac tttcaacgct cggtcatacc actcagctgc ctgccttagc
    13021 tcctgctgcg caaagtagat gttcgcgaga gccaggagtg gccgcaccat cgtgggatca
    13081 agctcggatg cttgtttcag cagcccgatc gcatccgcga cccgatcccc ccccagagcg
    13141 agaccggcac ggaggagata ggcggcagga tgttgcgggc gtgcggcgag taagctctca
    13201 gcgagcttgg cgacttcgtc ggttttccct gcggccagat agaaatcgcc gagggaccat
    13261 tgagcctcga aatcagtcgg atcaagttgc accacccgac tgagggaggt gaagatcgtg
    13321 gaaggatctc caacctgcga agccgccttc gcgagcttcc agtggatggc aggattatcc
    13381 ggagcaaccc gcgcggcgtt cttatattcg atcaccgcct caaggaactt ctcctgctgg
    13441 acgtacccat cgccacggct ctcgtgcttg gcggcccgtt ctgacggagc ctcggagcac
    13501 gagaccagga aggcagaaaa gccaatgaaa cataggcacg acaggcatct tgccatggcg
    13561 attgattcag gcccttcccc ctcagttgca ctgcgacagg gtgacagtta cagggcagct
    13621 cgctgagcag aacaagcttt tttctgcttc cgccgccacc ctaccagcgc gacgaatcca
    13681 gcaaccacta gcaggagagt accaggctcg ggcgcagagg ccgtgggtgc cgacacgaca
    13741 gcaagctgca acgtgcccag cccgctcaca aatcccacgg accaatccaa ccccgatgcc
    13801 agcgtcggca acgatagacc tcgtaggcct ccgctaattg tgcagccggt gcaagtcagg
    13861 acgccaaagg tattgcccaa tgacggcgaa aacccgttca gcaaccttat cgtgaggaac
    13921 ccattcaacg cgactgcagt tcctgtcccg tgcaccgcga gaagtccata gctattcaac
    13981 cctgccagat tgatacccaa tgtcccgcca aacccctggg cgtattgccc tttaatagtg
    14041 agcgttccca gcccattgtt gccgggcaga aggagtcccc cactattatt gaccgttccc
    14101 tgaatcgtcc ccgtcccgat caacgcgccc gactgaagag tgatattgtg cgcaaaagtc
    14161 ccgttgacga tggtgtgggc cgctgcggca ttttgcagat aagtgccggt acccgacact
    14221 cctccaacgg caccgacacc agctccgacc tcgaacgtcc cagaattcac caccgtgccg
    14281 tgattgatca gcgtagccac gccattgacc gcaacctgaa acgttccgga gttcaccaca
    14341 ctgccttggt tcgtaaaagt acccgcgttg acgagcgtgt tgcccgcatt cgtgaaggct
    14401 ccggtgttga cggtcgcggg ggtcgcaccg acccctgcgc cagagcgcgt ctggccatag
    14461 gtgcccgtcg ccccgacagt gatcgccccc gcgttggtca cgcgaccacc aaagctggag
    14521 aacgagccat cgatctgcgt cgtacctccg ctctgctgaa tataatgccc tccaactgga
    14581 ggtggtgaac catgttccag cctcgggaag ttgctgaacg acgtcccctc gccgatgcgc
    14641 accaggccag cgttggtaaa ggtgccgatg ttgttggtcg atacagctgg cagaccggtg
    14701 ctatccccgc gtgactggag atactgtccc gtcgccccca ccgtcatcgc gccttcattc
    14761 gtgacataac cacgggtgtc aaaactgccg tcaatccggg tagttccgct cgccagttgc
    14821 acatactggc ccgacgaggt tgggcggtcc gccagcactc cgctgcggaa ttctgtgccc
    14881 tccccgatgt acacccggcc cgcgttcgtg aaagttccgc cctggccggt acccgtgcct
    14941 ataggtgata gaccggccac ctgactgaac gaccccgtgg tggtcacggt aatcgctccc
    15001 ccgttctgca ctgcgaagtt attaataaaa ttcccgtcaa ttttcgtcgt cccaccgggg
    15061 aacggatgat agaccccgct tgtattggtg aaagtggcgc cttcaccgat gtgtacgata
    15121 cctgagctga ggaaaccgcc tttgttctgg aagacgcccg tgttgatatt cgtcgtcccg
    15181 gcattagtaa aggtgccgtt attgaccgta agactacctt gagtcgacgg aggagctggt
    15241 gatgcactct ggtgctcata gagaccagtc gcaccgacgg cgatccggcc atcgttaaat
    15301 acccgtccgt cgtttaggaa caccgcatca atctgagtgg tgcctgtttg agcataaatc
    15361 ccgaagggtc tatttatgaa ctcgccggct gtgatactga cctgccctgc attcctaaac
    15421 accatgtcac ggtttaccgt tgtcggcacc acggacgaac caacagcctg ccgctgaata
    15481 tattggcccg tctccgaaac tgcgaagccg ttactgttgt gctcggcagc accttcgttg
    15541 ataaagatgc cattgatctg ggtaacacca ccgccgttgt aataaaaggc ggatggcagg
    15601 ttgataaatc gtgatccttc caggatagaa acccgcctat agttgttaaa ctggtccgaa
    15661 ttgatgatgg tcccggaatt gatcacggca ccgcccggcg taaacggaag aaggatccca
    15721 ttgttgaact ggttggaatt ctgaaatgac gccaaaggtt cgatattgat cgtgcccaca
    15781 ttggtgaacg ggacaagatt ttcgcgaggt gtggacggag tgatagtaat cgtgttgccg
    15841 tcgggattgg gattcggttc gatttgaacg gcgaggccat ccccgacgtg ggtcaccagg
    15901 aggttcgcca ggagacagag cgcgacaggc caatgcacat gtggccgatg catatccctc
    15961 ccccacagac cgggcagctg gaagtcaaag tggcatgagc ctatacccaa ggctggaata
    16021 ttggaatctg ggaaatttac ttgagctcac cccacacgga cagacaggca gggccccgtg
    16081 tcttccggat ccttgcgcgg agacgtgaag cgtcaatcgt gaaaggcttt cggggactca
    16141 gagcccatga ctgagggcct tcgcgagcgg ccctctcttt cggagcatac gcgacatgct
    16201 agccgcaata cgccgagtca tctcagcctc gctgggactt gtcgattgcc cgcgtgatga
    16261 cttgcttttt tctggcgcgc tcagccgctg caaccgcccg gcccaaggcg cgtgcaaact
    16321 ccgtcgggtc ttccgtctcc gtccggcgct gggccaaatc ccattcctgg gtaatgccct
    16381 gcttcttctg ttgacgcttc actgcttcta ccgcacgcat caacgcggca gatacctgcg
    16441 gcgcagcgcc agcggatcct cggcgctgcg actcttccat ggcggcagtg acggcgtctt
    16501 tatgctgttc ccgccggccc gccgcggctt tccgcgcgac tgggtcggga gtggctccat
    16561 tcttctcgct cctggatgtt cttgactcac ctcgtactga tttcatgatc gatctccttc
    16621 acaccggatg tgattcaccc gccgccctcg gagagcaagg ctaccagatg accgcatgat
    16681 tatatcaagg catccggcag aagggagagc ccgggtcacc gttctcgcta tggactgacc
    16741 agaccctccg caatggcatg cctggtcagc gcgacggtgg aatggagatt caagtgatcc
    16801 atgatgcgaa atttatggaa ctcgacggtc ttcaccgcga tgttgagcat cgtggccacc
    16861 tctttggtcc ctcgcccctc cgcgatgagt tgcagcacct ctcgctgacg cggcgtgagg
    16921 gaggagactg aggtgcagac agacacacct gtcgcgtcgc tctggttggt caccatcatg
    16981 tccttaatga ggagtgacgt cagaaagtag tctccccgtt gtgcggcgct gatggcttga
    17041 ctcaattcag atgcaggcga ccgtttaagc aggtaggctg acgcgccgac cttaaacgca
    17101 tcggccacca tcctgctact ctcctgaacc gtcagaaaca tcaggccact ctccggggcc
    17161 agtttggtga gctgccgtgc cgcatccaga ccattgagca acggcagcag cacttccatc
    17221 acaatcacgt ccggacgtaa cctctctgcc tccaccagaa gcgaccggcc atctttcact
    17281 atcccaacga catcacaaga cggctcgatc aatgcccgaa cccctgctgc aacaatcagc
    17341 tggtcatcag caaggaggac tcgacatcga tcatatctgt gatcggtaca caccatcaca
    17401 ccactcccca catttcacca cgcacggagg ccaccatgta tggatcccga tggagcagat
    17461 tccgcttcgc tgaacaggtc ctgacccaag caagtgtttt gccatctttc aggcggtacc
    17521 tggagagtcc gacgacgctt taatatcgaa gccgtaaggg cggttgaggc tgcgggggat
    17581 cgggaaagca gggcctagcc tgtggcacgc gtccccaaca catcgccagc atacaggggc
    17641 aatagggggc agtgtggccg gtgcacctcc agcgttcccc atccacgcca ttgtgttcca
    17701 gaccccatcg cggcatactg cctgggggcc ttccaccttg aggacagaca ccatgcacgc
    17761 acgccggctg ttctatgcat tgatgctgat cgtcatgctg ctctccactg cttccggcag
    17821 ggatgcagag gcggccgaca caggcaccgt catgaagggg aatgccggac aggggcaggc
    17881 actcttcaac ggcaagggca tctgtcacta ctgccacggc gtggacggag tgctcgacaa
    17941 gaagccttcg ctcaagcctg acactgcggc cgccatcgcg aagctgtccg ctggggctcc
    18001 cgatcttcgc aatcgcgccg ggctgaccct gaaagacaac aaagcccgct tccgggcgat
    18061 tcgtgaggga catccgggaa gtggcatgct gccggacacc acgctaagcg accaggacat
    18121 cacagacctc ctagcctatc tcgccagcct ccggcagagc cagaccactc ccggcaagag
    18181 cccctactaa gcggcccgtc cccttacgcg ccgccgggaa cgactcgctg attcatccga
    18241 tggtcactga accgacgccg tcgtgaaacg cgatgggcgt gcccagcgcg gcacacttgg
    18301 cctgcatggt tttctggaga aacagggcgt cctgatgccg ggcgagcctc acgcggcaat
    18361 gctcaatgcg aacgggatga agccgaatgt gcgcgacacg gcctggctcc acgtcaagga
    18421 cgaacaggaa cgatagatcg ttgcgttcat tctcatccac catgtagtcg tcgatgaaat
    18481 cgccgctcga atacagaatc gccttcccat ggtagagttc gatcccttga ggactgtgat
    18541 tggaatgccc ccaatagaaa tcggccccca gatcaagcag ctcccgagca agcgcccgca
    18601 tctcaactga cggcacaccc cagttaggtc cgacgtgggc gctgaccagg acgaggccgg
    18661 cttgacgccg ggctcgtctg atcgcctcca ccacacgcgc tcgatagggc tccttgagcc
    18721 cactacgggc gtaggccaca tagacgactc ccggcttgac atcggcggct tcccagtccg
    18781 gctcattatc ggtcagcgcc agcactgcaa cccggctccc ggtcgacgac aagaatgccg
    18841 gctccatcgc tgtcgccaga ttccggccgg cccccgcatg cgtgatgccg gcccgatcga
    18901 gtagtccaag gcaatcgagc aaggcctccg ccccgtaatc gagaacatgg ttgttggcca
    18961 acgtcaccgc atcgatgctt gccgacttga gacagtcgat cgctctcgga tgggcacgga
    19021 aatggaacgc tttcgattgc ggacgccatt ctcgcccgcg tgcgctaatg acgcattcca
    19081 ggttgatgac tcgaaggtcc gccgcaagca ggatcggcaa cacatcgccc cagacggcct
    19141 caggccggag ggactggttc cggatcacct cttgatccac catccgtccc agcatcacat
    19201 cgccggtcaa tgcaatctgc atcggtctta ccgatgagtc ctcaggccga tcgccccgcc
    19261 agcgcggcct gaatccgttg cttcacatcc tccggatcaa acactccggg gaaacgaatc
    19321 gtcgcgtcgt cctgccgacc tcgaacgacc acagtcccga tccccatcag atcagccagc
    19381 ggcccctgct tgatggagat ctcgcggatc tcccgatacg cgatcgattg aatgtcgcgt
    19441 cccgtccagc cgttccgcag cacgactcgt cggcttgtca cgacataccg cccccaccgg
    19501 cgcaatgcgg cggctgttcc caacaacgtg atcgcgccga ccagccaagc actccatcct
    19561 gacagggaag acctcacaag gagtgcaacc cgcagcatca tgatccccac gatcagatag
    19621 agccagatga attgactcca ggccgcaccg ccttcccaga ggatcggttc tttctctcct
    19681 gtggcattct catccatggc agtgacctgg gacttttctc tacagcccga ctatctggtt
    19741 ctgtcgcgga gttccccaca cctccaccgg atcacagaaa caggtacaca aagagccttg
    19801 tgcgtttatg cgcagatacc gcctacaagc ctcttcactc ttactggcct cgctcttgct
    19861 tttcaccgac tatgagtcgc cttgcactgt gctgcaagtt tcggacaatc gattgttgcc
    19921 tgagtgcccc tccgctccct gaccaccaaa ctcagtcaaa ggaacgtcat ggatcagcaa
    19981 cacccatccc cttcccgctg ggccatcgtc cttgcgggag gcgaaggcac caggatgcag
    20041 ccactcgttc atcgctggct cggattcaac cggcccaaac agtattgcgc cttcgtcggc
    20101 tcccgctcca tgtttcaaca taccgtggac cgcgcggcct tggtgacagc cccggaacgc
    20161 atcgtcgccg tggtggggcg tgatcatcgc gacgaaattc tgacacagtt acaggggcga
    20221 accatcggac aggtcatcta ccagccacgc aactgcgaaa cggcggcggg actctttctc
    20281 cccttgacct acattcatgc ccgggccccc ggcgcgacgg tcgtcatctt tccctccgac
    20341 cacttcatcc atccggaaga ccgttttcta gcggccgtac gccgcatcac cgacctggcg
    20401 gagaccatgc ccgatcgact ggtgctgctg gggatccgac cggatcgact cgaaatggaa
    20461 tatggatgga tcctccccgg caccgcgctt tcttcgccgg aggaggcacc gatatgcgag
    20521 gtccattcat ttatggaaaa gcccagccgg atccaggccg tcaaggcgtt tcgccacggc
    20581 gcattgtgga atacctttgt gtttgccgcg cgagccgaat tgttgtggca ggtggggacg
    20641 cgttgcttcc cagacgtgat gcgccgcttt gaacggctca gaagcgtgat cggccagtcg
    20701 caggaaacag cggtgctcga cgccatttac gaggacatgc ccgcgtacaa tgtgtcgacc
    20761 accctgctcc agcaggccgc cgagtccgcg gtcgtcgttg agctgcagga tgtcctctgg
    20821 agtgattggg gccaaccagc cagggttgcg gagaccctcc gccgcatcgg ccgcacgccc
    20881 gcatttccga tggaatgtct cgaccctcct ttttccacga agcctctgac cggcgccgat
    20941 cccgccccgg tcgtacagcc ataaaaacga gaggcacgct cctatcgcga aaaccggccc
    21001 atcaaggatg cttccgccag tacgtgcccc ttcatcgcat cgaccacctg aactttggtg
    21061 gcccgcgatt taagcgccaa ctcgcaatcc aatgcataca acttgaagta ataccggtgc
    21121 ggcttcccgg gcggtggaca cggcccgcca taaccgattt cctggctgtc gttcagaccc
    21181 tgctgcgccc cgttcggcaa ggtctcctga gtcggagtcc cttcggccag gccacagaga
    21241 tccaacggga tgttgtacaa cacccaatgc acccatgtgc gacccggcgc atcgggatcg
    21301 tcggcaatca gcgcaaaact tcgcgtgccc gccgggggat ggttccagcg caacgggggc
    21361 gacagatcct cgccttcaca ggtatgtttc ttcggaatga gttccccctc cttgaacgca
    21421 ctacaggcga gttcgaacgg catcacagac tccttcgtgt ttcttcggga tgatgacgta
    21481 gccacacctg cttgggtcta tcaggttgcg gaaaacctcg attgtggcgc aatacgttat
    21541 gatcaactcg tgcgccgtgt cggtatcagg atcacccgca ggatgcgcaa aaaggctgtc
    21601 cagcaaggcc gcagcgagcg aggggctcat cctactcctg tggtacgatg agccgcgaag
    21661 cgatgcgaga acgccgctgg cggacttttt ccgcatcctg ctataaccag attcgctcgg
    21721 cggtttgtgc ctgcactccc cacaaggcgc tcacgggcag atcgagaagg gaatgcacgc
    21781 cggggctcaa ccccggtaac gcacgggcgg cagccagcat catgtgcgcc gtgagaagag
    21841 cttcatcgaa gcgggcttca aagagaaacc gctgatgccc cgcacgcccc ggcgcactcc
    21901 gccgctcaag cgcgactccg cgcccttcct gttcgagcgc ggccacactg tcgaccggaa
    21961 ataccagcgt ctcctctccg aggaagagcg gatcggcgcg aatctcagcg gccacgcgat
    22021 ccgcatcgcc ccccttctcc aactcgacat agacataccg ttgccgcgtc ccgtttgccg
    22081 caacctgttc cgtgcaaagc gcgtccttga cgccggccac ccggcgggcc atcgcggtat
    22141 gatgaaggct cgcggccacc cgatgcctca tctcgctctc gccctccggg gcgaggagcc
    22201 cgaacaggga gcgcatgacg gacagtgccc ctggatccca gccggcccct acgatggctg
    22261 gaacatcgaa acgagtggcg aaccggtcga tcgcttcccg atgtgcttga aacgcctctc
    22321 cgtgcaagag cgcgcattcg atgatcggga gaccatgctg gaggcagtcg tgggcgaccc
    22381 cttcgacctg gtcgatcggc acgcacaaca gcgccgcatc catctcgtgc acctgcgccg
    22441 tgtgcgacac gaccggaatc ttgctgaaca cctccggcaa cggctgagcg agactgtcga
    22501 ggcgtcgaac gatggccgcc aagtccacat ccttgctcgt gagcagcaat tctgcgcaag
    22561 cccgtccgac ttttccgaat cccaccaccc cgatgcggat agtgttcttt tcattccgac
    22621 cctttgggat taccacagtc caaaactcac atccttgaag ggaatcgttt cccattgctc
    22681 gctttccagg ccgaggattt cgatgtcgag gtttgacgga atgactcgca ttcctttggg
    22741 ccggacccag ctatccgact tcaacgactt cacgtacaga tgaggtctgg cttgggcata
    22801 atcccgttga tggaagagct cgagtagccg caatgcatcc ggcatggtca acgtcatact
    22861 gtcctcctca caagtttcaa tcacccgtac catgcagtcg agtgagcaat cgcgatgcct
    22921 gctgagtctc gcctgtccag ctatcggatc gacggcccgt ttgttcagca aactcgccgc
    22981 tatggagtgt ccgcaagagt agcgccgccg atacagggta ctccagcatc cctgctatcg
    23041 agttcatgcc tgtgcccttt cgcggcacct gcgcagaaat gcttgtagcg gatttcctca
    23101 catggcccca tcaggaccgg gagcgatcat tgcatctgtg tagtgcggca atcacggtga
    23161 tgaactcgcc atcgcaagag gggggaaccg tccgggtgag acgcatgggg ccatcagtcg
    23221 tatgggtgct cctgctcgcg ggattcttgc tgtcagccgg ctgtatcggg cctctgcaac
    23281 agtggggaga agaaacgaca ttccgcccca aggccacctc ctttgccccg gccacgctga
    23341 agcacgagca ggttgccgtc ctgaacgcgg tggtgggatt cggattggaa gggtttgccc
    23401 atcaggtctc ccgctcattg tcgagtgcct tggatcagcg gccgactctg accacggctc
    23461 tgccggtcca cgaagcgttg aaccgcatca accggggaga gttgggagaa gagtatgctg
    23521 ctatggtggc cgactatgtc agaaccggta tcctgaatcg ggcggggctc cagaaaatcg
    23581 gacaggccat tcacaccaac tatgtctttc aacccagcct cgcctcgttc aaccaatcca
    23641 tgtccgggcg cttttccttc tttggcctgc gtgtcctgca aacccgcgtc accatgctcc
    23701 gcatgagcct gcaactgtgg gacaccagaa cgggcgagat cgtctgggaa tcctccggtg
    23761 aagccaccct cgcgggggaa gacgttcgcg agttccgcat tcccttcgat gagatcgccc
    23821 gccgcttgtg ggctcacatg ctcgacgatc tcttcaagga tgtaccggtc gaataacaga
    23881 tctcgacacg gctgcctgct cgcacttgcc ctggtctcgc attcgtctgc cgcaacacca
    23941 gccgacacca tctgtcgcca ttcgcaccag ccgccttgta gccagtgggc aattcacccc
    24001 caggccctgt cgcccatcac aagcccgtca gattgcggca tcgagtaacc gtttgttctt
    24061 tcgtcatatc gaggacgcgt cgcacagggc atgcgacttg ctgataactg atcgtgggca
    24121 cgaacagaac acgtaacaga tcccgtgtaa ggccggcatg agtagacgat gtgtatctga
    24181 aggtggccct tgcaacactt cggaactccc atcatgcgcc tatctcattt cctccaagta
    24241 atctccgcag gactggcctt cgcgctgctg ctcgtcagcc cggccgacgc gacattagag
    24301 ggcgcccctc cgaccaccgt ggtccgcgcg acactcgaca cggtctttca tatcctcgac
    24361 gaccagcagc tgaaagtgcc cggccaactg tcccatcggc gacgcctgct ggaggaggtc
    24421 atcgccgagc ggttcgacta cgaagaaatg tcgaagcgaa ccctggccgc ccattggaaa
    24481 ccgctcagtc acaccgaacg acaggaattt gtgcagctgt tcaagacgtt tctctcggac
    24541 caatatgcgg cgaggatcga gggctacgcg ggcgagcggg tggtctatct ctctgagcgg
    24601 acggtcgacg gctatgccga agtccgaacc agactggtgt ccgacaagct cgactttccg
    24661 atggattatc ggctgacgaa taagagcggt acctggtatg cctatgacgt gattgtcgat
    24721 ggagtgagcc tggtcatgaa ctatcgcagt caattcggca agatcatcgg agagtcgtcg
    24781 ttccaggaac tgttgcgccg actgaaagag cggagagtga gcgaagagaa aacgaagacg
    24841 taagactccg gaaagccgat gcggcgacct cagccgatcg acgtggggag ctgatacccg
    24901 ctgagccggt tgaggtgacg agagacatcg tggtaccgag ccggcgtcac gaggtaacaa
    24961 tagtcgaaaa gtcgatccgc cgcctcggcg gaaatcccct gttcgcgtgt ttggagcatc
    25021 tccatgaatc gattcatggc ccggtacacc gcttcgctct cgccgctgat gaggtgcgcg
    25081 cgcaccatcg tcagttgtcc aaggtagagg gcgaacaggc gaccgctcgc tccttccttc
    25141 gcctgttcca cgaggaccgc ccggatcatc gcatcaagcc agtcattctc gatcgcagcg
    25201 gccgtactcc atcccccggc caataacagg atcacgcagg tcagcaagag cccgcgtgcc
    25261 atcgttcgcg tgatgtcata agagatccgc atcttgcccc tccttccttg tgagggttcg
    25321 tcgtcgcgcc tgtctcctct gggtcatgac aatttgtagc cgaactgccg cagcagagcc
    25381 ttgcggtcgg cgatctcggc gtcgccttcc acgcccttcg gactcgcccc atccacaaca
    25441 cccagaatac cgcggcctcg ttccgtttcg gccacaatca cctccacatc gtttgccgtc
    25501 gcacaaaaaa tcgtacagac ttccggtacc tggcgaacgg ccggcagcac attgatggga
    25561 aagcagccct tcagcagcac gagaaaacta tggccagcgc cgatcgccga cgcattcgtc
    25621 cgcgctgccg ccaccaaggc ctcgtctgtt ccgctccatc gaaccaggca gggtcccgac
    25681 gcctcgcaaa aggccagccc gaacttgatc cccggtaccg ccgtcaccag cacttcgtgc
    25741 aggtcttcca cggtcttgat gaaatgggcc tggccgagaa tgagattcac gtactcaggt
    25801 ttttcgatcg gaaccgcctt cagggtgacc gccatcatgc ttccttccct gttcccaacc
    25861 aacgtccgcg cacgtcctgg ctgctacggc ttcacgatca agaccggaca gggtgccagc
    25921 tgcgccaccg cctgggacac gctcccgagc gcggccgcct tcaccccggt caggccacga
    25981 gatccgacca ccagcaggtc cacagcatcg cgccgggcag ctcgaacgat ggcctcggcc
    26041 ggatggccgg caatcaccct ggcgcgcacc gtgatcgacg cgtccagcaa cagacgctcg
    26101 catccggcga tggccttgcg cgcctcagca cgccgttgac gcaggaacgc cgcttccatc
    26161 ttacgactct catcaggctg aaaccgttcg aggaccgcct tctccgcacc cagcggacgc
    26221 acgacgctca cgatcgtcac ctcgcgtatc ccgggaagac ccgccacaac acaggccgcc
    26281 cgctgcgcat cctccgatcc atccgtggcc agcatgattc gcaacccgcc gccggcctgc
    26341 accttgccag gcaccaacat caaactgcag ggagcatagg agaggagccg ttgagcgact
    26401 ccaccgatcg tgaaaccagg ggaccggtag agcccctgca tgcctgagac gaccaggtcc
    26461 ggtcggcgct cctgaatgac gtgcagaatc tcgctgctcg ggacgccttc ccgcacaatc
    26521 acttccacgt cggcaaagga ctcctccagg agtcggcgca cgcgatcaag cagggcctca
    26581 accatcgcgc tctgctgctg cagcagcaac tgttgcccgc tgacgccgat gctctcaggc
    26641 acctccagcg gagccagcac atgcaccacg gtcaccaccg tctgcttcga atgtggaaaa
    26701 gctcgcatcc atccgatgat ccactcggaa cactccgact catcgagtgc aaccaaccat
    26761 cgcatcgtca cccctcagct ccctcgtcgc cggcgcaata gcgcgcctcc tgtgttacct
    26821 agcagcatag gatatgccac gcacaaaccc ctgcgatggt tgaatttccg tttcacccct
    26881 gccctaaatt gccacaggcg cccggcaggc cactggggca cgagccccca ttgacctcgt
    26941 ccaaaacagc attatttaag aagtgagcgg aggtttcggt gagaggaaca gagacgggaa
    27001 ctgaggtcga gcgaagaggt ggtgagcagg cccgcaggtg ccaagcggga agcctgaaac
    27061 ctctccaaga tctccactgt tgctgacaag ctctctgaac ctgcctccgc cctgcttttt
    27121 acccttctca ggttctcaat tccgtaccaa aatgatgagg cgtgacgctt ttgagattcc
    27181 gtcgtggatc ttcgtggaat ggctgcacag cgctgagtag agggtgatca aggaactgga
    27241 agtattcagc aggtgagaca aaaaaacggc ccgcccttgc gtggaacacc ggggctgccg
    27301 accagccggg aggagttcga caatccttca gtcgccgtag tggctgattg tcccgcgcag
    27361 agacttcata tgcgccgcac cggcggccgt gcctaatggc accgcgaaac actgcgcgtc
    27421 ccctgaaccc tctcccgcca ctgaagcaga agcggtgccc tttgcacgct ggctgcacaa
    27481 ggcgagtcac attcgaacaa ggctgccgac ctaaagaagc tttgcgcctc ctttccttca
    27541 cttttaagat agccatgaag ggcgaggaat caactgtagt gcttcagact cgacctggca
    27601 gtcagcgtca gctatcgacc caaagcagaa gtacacgacc gaccgctacc agaagggggg
    27661 gggcttacgc cgatccgcat aagaacccgt gatacatggt cgatgggccg tcactcattg
    27721 catcaccgtc aatggtggga tattgttcac gatcattgaa gctgtgtgac gcggatagaa
    27781 catggcagat ctggttctta tatggatacg cctaaacatt gataggccac acgaacccat
    27841 cgtctctacc cgacggagaa cggaatagca atgcttacac cagttgagat tgccgcactg
    27901 attaccgcga ccaaaggtgc cgtcgacatc ttcgataagg tagcaggcca agtgaaaagc
    27961 gtgctgacga agcgttctaa ggaagtcgct ggcgacgatg atcagtggcg ctacaagatc
    28021 ggatccgatg cggcagctac aagtatcatc gtcaagcagg acaacagagt ggttcaaacc
    28081 cttaaggggg acgagcttgc gaagatgctc agtccaagcg acctcaggct catcaagacc
    28141 tacgaatcga agatgagcga gtactacgat ctttgggaga aggtatacga ggaaaaagac
    28201 gcatccaacg atgccctcgc gaacgcaaag acggagaagc aactgaataa gctcatcgcc
    28261 aagatgaggg atgagctact tgggatactt cgtttccttg agcaaatcgg cgttcatctt
    28321 gacgatcact acatgcagat tcgtcattta gtgcaagatg taaagtagta gcttgtgtgc
    28381 ctacgtgtgg ccatatccag attggtgcag cctaatctct catcccagcc gaccgccttt
    28441 ggcggcggca aaacatgaaa gttgggcggt aggcaccaga cggttgcttg gcgaacgggg
    28501 cgcaaggcac tcagagcagg gcgacacttg cagcaaagga ggcgactcat ggcggcaaaa
    28561 aaacgcatag cgaagatgca cggagagaaa cccacccaac tcgctcgcgc gctatatatt
    28621 ccccaaaaga tgtcccatcg ttcgctcagc gcatcagtgc cgaccgatgt tctggcgagc
    28681 acggcccccc gagttccact gccatcagtc ctgtgctgtc cggcgcatct agtgcgtcgg
    28741 gatgagtttc ctgtcgttca tgaatctccg aggcaaattt cgcagcggtc aatcagagtt
    28801 accacgtcat cagtaacaac tggagaagcc acagctccaa taggtgaaat cccgtcggca
    28861 gaggtctttc agcagcgtgt gcaggacctg ttggatgacg gccatggcac cacgcatacg
    28921 cccggtgggg atattgcggc ttcggatgcc ttttatgatt tcgacgagta tctctttctg
    28981 cgcaatacgg acgaccccta tgtcgacccg cgtgtggcgg gttcgacgca ggtcattcca
    29041 gccgatcctc gcatgagccg gttcacagcg gcagcacggc agggtattga gttctttgta
    29101 gactttgcgg tgatcaattc gtcagttccg tataaccaga tcagccaggc atccatcgcg
    29161 gcactcgagc agctcctatt tagctggtgg tcgtcggtct gcaaggtgag ggcgtataac
    29221 gaactcatgc agtacagccc aactgcgatt cgtacgaaac tcttccagca acagaccaat
    29281 ctgacccccg agcaatatta cggctccgca ctttatgcac ggctcgctga cctcaagttg
    29341 gctgaaggca aggggtattt tgacggaaac ggcgattcct cccggcttgg ggatctggtc
    29401 agcgccgatt tctgggaacg gctgctgacg ttggtattca aggagcactt tgccgggatc
    29461 ccgacaaccg attccggcga gcgcggcgct gagtattcgt tcgatccgta tccagctaat
    29521 gaggtgctgt tcggtcttca agtcattcac cgacaaagct ggcgctcttt gggcaacggg
    29581 cgcggcgagc tcgtcaagag catccccctg ggaccgcgcg agacccagaa ggtgagcgtc
    29641 aaggtcacga cgcgcacaaa gctgacgcgc ggatcggagg aggcatcatc gttcgagacc
    29701 agttctgaat ccagtacttc gactaaagac accgcagaag ttgtgtctga agcctctgag
    29761 aagatgaaca agcatgccga ggcggaggtc agcggcggtt atggcccctt tgtgcaggcc
    29821 aaggtctcgg gtggcatctc ccaggatctg gcgtcgtcca gtcgccagac caagaaccga
    29881 ctgaacgagg tgatgcaaaa aactgctggg cgcatgaaac gcgacacgaa ggtgactgtc
    29941 agtacggaga gcgaggagag ctacgaggtt tcgcgatctt cggaactggt aaatcccaac
    30001 gacgaggtgg ccgttaccta cctgtaccat cggcttcagc agcgctactg ggtaagcacc
    30061 cgcatcgcag aagtgcattc cgtcgtattt gtgcctgaac ggctgcccga accgacggaa
    30121 atcgatgaaa actggatcgc gcggcacgcc gacacaatag cgggtgcctt gctggatcag
    30181 ggatttgcag gaattctcaa caccatccgc aaggaaccct cgactctcac ttcaaccttc
    30241 agtactgcat ttcagcgtgc cgctgacacg gccatcagtg caacgggtaa tttcaggaat
    30301 tataccggcc agggcacgat gccggatttc ctggcttccg gtcaacggtt ccatgagcga
    30361 gacatggaac ggcgcaatgc gttcaacatg gatcgggcgc gccgacaaca ccaagccaat
    30421 gcgctaattg cccacatttg ccggcacatt ttgcactata tgcgggcaat ctggcgtgcc
    30481 gaggattatg accagcgcat gcaacgctac agtcgcctgc gcgtgccaac ccgctggtat
    30541 ttcataccac gcacacccca gtcggcaggt gctccatccg ccacaccgct tcaagttgat
    30601 ggtttctatg tggcggacga ttccagcgct aggcccctta cggaagtcat tgacccgatt
    30661 ggaccgattg gctatctgtt caactgtgcg atttaccgtc tacgcgatga cgacaggctg
    30721 gccaacaccc atcaggcgct agcctatctg cggtcgcact atgtccgatt cagtgtgcaa
    30781 tgcacaccgt cggtcggggc cggggtgacg gtgcggcagg ccgtggctcg agcgccgcag
    30841 agcttttcct cggattacgt cctgacctat cgtcagcagc gcgtcaagtg gctcctgccc
    30901 atccctaaca ggtctgaggg cgactggttt gaggcatcga agctcgaaga cgggtcaatc
    30961 gagattctgg gtatccgcat ctggattgat ggtacgccag cggaccgtgc cgagttgaag
    31021 atcgcagtgc gtgtcacgag cgagttggaa gatccgcagg tccgtcatac catgctgcag
    31081 tacccccttc ccctccatag caatgagccc ggattcttta ccgcaagcat cctagccaca
    31141 atggccgacc tgctgccgcc ggttgccaca gcactagcgg gcacgcacga ctgggcgcaa
    31201 ttgacggagg cgcaacgtac ggtggtacgt gcctactatc acgaatggat cgtattgcgc
    31261 gacagcggtc gtctggtgcc gattgagaca agcaatgtgt tgctggatct ggaagtaggg
    31321 ccctcgccga tcctggaacc gttcaagcgc ctgcatcgtt acatcgatgt catgcgtgag
    31381 tatgagatca tgcgtaaaaa ccatcttgag aacggccgcc gcgaggcact attgcaggcc
    31441 gggcggctcg gagaccctga gatagaacgg gtatcagtag tgtccagcga tccctcgctg
    31501 gcgaagttgg tcaccgtgac ggacgacacc cctggcaact gattccacaa ggagaacatt
    31561 atggcgacaa agagaacaac cgcaaagccc tcggcttcgc gcaagcttga ctcggccaga
    31621 ttgcgcgact tgctgaccac ggtgcctcgc ttctcgaaag cgaagctgaa cgacgaggtg
    31681 gcgcgagccc ttcgtttcct ggaaacgcgg ccggagctga aggccaggct tgattcaggt
    31741 gcattgacgg ctgtgcagct gctggccatg gtgcgcgccc aggccgaggt taggatgggt
    31801 gaatcgagcg accagcacac acgacgcgag gtggacaggt atctaggcag cgaccgcacg
    31861 ggctgacagc cacgaccgac atcgatgcag gaagttatca atggctgagc cgacattgga
    31921 agaagcggcc acccgactcg gctcggtcta tgccgagctg gtatatgcgg gtcacgcgct
    31981 cgacgaacaa acattgccca actggatcga gccggaacga ctgcgcccgg tcggggggtt
    32041 tgatatcggc acaaagttgc tgttggctct tcgcaaatcc ccgctgtact tcacgcaact
    32101 aggggatgcg gttcgtcggg aggcggggta tccgaacctc cagttcgaca acctgcgggc
    32161 cagccgtttc cagtatgaaa gggacctgcg ggtatttcac aaccggctga agccggcact
    32221 ggcgaaatat acacagggag ccatgatccg gcaggaacag gagctgcgca agctgcgcgg
    32281 ccacttcggg aagccgggaa tcgaagatga actgcacacg agctttcagc gtatatggtt
    32341 tgcggtggag gaagtcctac cgggcggggt acgtccccga tcgggagaaa acgagagcac
    32401 tgccgcaggc gctgacaatg cgccagcttc gtccgatgcg ggagagacgt cgtccgagaa
    32461 tctgcccggt ggcgtggccg gacccgttga tcccgaggcc ggccagctca acactaaccc
    32521 ggatgacatg cccaccgact ttgtcgaacg ataccaaatg ctacgcgcca tcaaggtcga
    32581 gcgagaacgc ctgcaatccg aaacaaagga gggcggtaat gcgctgaaca atgagatcgc
    32641 cgacctgctg gccacgatcg ccgtgcatcc ggaggtcgtc aacatcgccc gccactacgt
    32701 ctggtcgccg atcaagggaa cggaatacac ccagaccgcg gtggatcagg ccattgcagc
    32761 cgtcgccgac cttcgcgaac gcctgactca gcgcgaaccc gacgacattt ggaattacgc
    32821 accgcttgtg gtcggaacgc tcacacaatt aaaactgcat gaattcccgg cagttcctga
    32881 attcagcgtg gcgcttggtg ccatgctggg ccggacaaag acgaatgttt atctcggtgc
    32941 agccacgatt ctgatcgtgg ggttatcgct ggtctttacc ggcccggtca gcgcggtggt
    33001 ggtcggttcg ctcgatctgg cgctcgctgg caccaccctg ggtatcacac gactgcgtgc
    33061 acacgagcag gaaatcgcgg ctacggcatc ggatttttcc cccgatgatc agaaactggc
    33121 cgcccacacc accagcaccg aaggtgcact ggatgcggcc ggggcgctgc tgtcggctat
    33181 aaccctggtg ggatcagttc gacagctgac gaaagtgcgc gagctgttga ggatcgaatc
    33241 gaagggccta aaggtaatcc gatcatcgaa gcgtgaactc gacacggctg accgtgccgg
    33301 aggttttggc aacaaggtta aagacgcatc tggacgagac gctacatcct atgagcttga
    33361 agaccagatc aattccaccc ttgggaatcc aaatgctctt actgaagagg taatgcagcg
    33421 gagggcagaa agaactggga cacggacact ggttggcgag aaatgggaat acacgcccga
    33481 taagatgcga cgccagggtg ccaagcccgg tcccaatgat cccgaatttg gcttgggacc
    33541 acaaccggac tttgaattag atctgggcga tggtatcgag ctcgagttgg cagattggcg
    33601 caaaaccggc gcggccgaaa gtcctgtgca caaaagacca gatttccatg agatcgagcc
    33661 caaatctcta gagagtttac cagccgttac ccacaagcag aatcagttgt caggagtgcg
    33721 agagattacc gaagccgagg taaccgccgc acaaactgca tcgcgccagc cgccggactt
    33781 agaagccctg tatgtgcgcg aacgtgtgac aacgcgcgag cagattacgg ggttgtatga
    33841 gcgagacccg ttctatccga agttcgaaga gcaaaaagca atgctgattc agtcgggcgc
    33901 aaaagatccg ataatcccga attacaaatt ctcaaagaag aacccgcccc acgtagacca
    33961 tattattccc gagaatcaag tgcgcaagtt tgagggcttt acggaactcg atatcgagtt
    34021 ccagcgtgag gtattgcaca tggacaagaa tctagagttg gtgagttctt ctgtcaacct
    34081 ttctcgcgga gacatgccat atagcctttg ggatggcaca atggaattca agattccacc
    34141 ccagatacgt aaggccaaga ttgcacaaga aaaggcgctt gccatcgaga tccagaacaa
    34201 gatctataat ctacttgagc ggcagcgaca gcttcgaggt acgacacgac cgtcggcaat
    34261 gccaatcaaa gctggagctc ggttaacagg cgacgtttcg cgtggtttga ggctcgtcaa
    34321 tgagcgcgag actaagcagc acaaatccgg gagtttagat ccaaagaaat ggtaatcgac
    34381 ctacaagtta ctcgggacct ctggcttgag gtcctattcg agccgctcgt gcgcgtggtc
    34441 aagttgctgc gatgagcggt tatcgggact gcgacctgcc gtccaacaac atcgaggggc
    34501 ccgctttatg gcgttgcgat ctcggtgccg aacttccgct tctggacgag atcggccttt
    34561 cgaggcccta gttcctgcgc ctagtcctgc acggccattg cgattccccc ctccaccgct
    34621 cttatcactc tcttgccgcc gccggcgcaa cgatcagccc ctccctcagc aacacgcgca
    34681 tcgcttcgtg ggcggaacgg atcttcgatt ccgccagaat gtgcacctga tcgcgtgttc
    34741 tggtcaccct cgccagaccc tgctcctgcg ccttaattgc cgtggccacc gccagacgca
    34801 tcggcacgtg ccattcgtcc attgtcggca gaatgctgcc ctcatggatc ccgcgctctc
    34861 tggcgcacag cgccagttcg tgcgccgcgg cgatcgccat ctcatcggta atcgccctcg
    34921 cccggacgtc gagcaggcct cgaaacatgc cggggaacac gagggagtta ttcacctgat
    34981 tggggaaatc gctgcggcct gtcgccacga tccgcgcgcc tacctctttc gcgtcccagg
    35041 gccagatctc gggaacggga ttggcacagg cgaacacgat ggcatccctg gccatgccct
    35101 tcacccattc cggcttgatg atcccgccgg ctgagaacgc cacacagaca tccgcaccct
    35161 gcaaggcttc ggcaatgccg cctcgcaaac cggtcgggtt ggtgtgaaga cagaccttcc
    35221 attgttcgct gaaggcaggg tcctgctcat attcccgccg atgcgttccg agaatgcctc
    35281 ccgcatcgca ggccacaatc cgggcaggat ccgccccgaa gaccttgagg aatcgatagg
    35341 tcggcacgtt cgccgcgccc atgccgatca tggcaatccg aacctgccct atttcttttc
    35401 cgacgacctt caacgcattg atcagacctg ccaggagcac cgttccggtt ccttgctgat
    35461 catcgtgcca gaccgggatc gggcaatcgg cctgcaattc gcggagaatc ctgaaacact
    35521 tcggcatcgc gatatcttcg agattgatcg cgccgaagga tggggtcagc agccgaaccg
    35581 ttcgaatcaa ttcgtccgga tcgttcgtat cgagacagat cgggaccgca tccaccccac
    35641 cgagatattt aaagagcagc gccttccctt ccatcacggg aagccccgct tccggtccga
    35701 tgtcgcccag gcccaacact ctcgtgccgt ccgacacgat ggcgatcgtg ttccccttgt
    35761 tcgtatagtc gtacacgagt tcatgatcgg cctggatcgc cttgcacgga gccgccacgc
    35821 ccggcgtgta ccacaccgaa aaatcctcca gactccggat cgcgcacttc ggcaacgtct
    35881 gcatctttcc cttgtagtac gcatgcagcc gcaaggcctc ttccgccggt ttcgaagcct
    35941 tcgccaacaa ctcggctttg tccgtcatca tagccctcca tccgacatcg aacgcatcac
    36001 aggatcggca ccggccaacc cgtctccctg cccgctcaac cttccatggt cggcttcaga
    36061 agcttcctca attccgcgta cggccttgtg ttcagtacat ccgttttctc aagccatccc
    36121 cgtctggcct gcccgatccc gaagcgcgca ttctccaagt cgagcatgct gtgcgcatcc
    36181 gtattcaccg ccagcaacac accctcctcc ttcgccatcc ggcaatggat gtccgtcaga
    36241 tccagtcgtt cgggatgagc gttaatttcg agaaaacacc ggcgctcgcg cgccttccga
    36301 ataatgcgaa gcatgtccac atcatagggc tcacgccggc cgatcagccg cccgctggga
    36361 tgggccagga tagagaagtg cggatggtcc atcgccttca tgatgcgctc ggtctgccgg
    36421 cggttcgaga ggttgaagcg gctgtgcacc gcgccgacca ccagatccaa ccggccgagc
    36481 acctcatccg gcaaatccag attcccgtct tctaaaatat cgacctcgat acccttgaga
    36541 atcgtgatcc ctgacagggt ggcattcaat cgatcgatgg cttccgtttg ctgcagcaac
    36601 ctggcggagt cgaggccctt ggccatggtc aaccggcggg aatggtcggt gatggcgaaa
    36661 tatcgcaatc cgcgcaagcg ggcggcctcc gccatttcct ggagactgtg ccgtccgtcg
    36721 gtcgccttcg tatgggcatg gagatcgccc ttcagatcct gcaggtccac gaggtgcggc
    36781 agtcgcccgg ccttcgccgc gtcgatctct ccccgtcctt ctctcaactc cggtggaatc
    36841 cagggcaggc cgagggaggc atagaccgat tcctccgtgt ccccggcgat aggcttgtct
    36901 ccacgaaaca cgccatattc attcagcttc aaaccgcgct gttgagccag ttgccggagc
    36961 acgacgttgt gcgctttact gccggtgaaa tagagcagcg ccgctccgta actgtcttgc
    37021 ggcacgactc tgagatccac ctgcaaaccg ctctgtaggc gaacactgga ctttgtctcg
    37081 ccacgcgcca gcacgtcgcg aacttcctga taggccacaa accgatccat gacggcgcgc
    37141 cccgcaggag ccgtgaccag aatgtccaga tcgccgatgg tttccagtcc acgacgataa
    37201 ctccctgccg ctatgacccg gctgactcct gacgattgtt ccaggtacgc caccagagcc
    37261 tcagcagagg gaatcgccac ggcccgctgc acccgtgggg cttctgcaag acgcgtggcc
    37321 aatgcatcga gaatgtgttg ctcggttttc tccccgaatc ccgccaaggt ccggacccgg
    37381 cctgcctgcg ccgctgtctg caattcggac aggctacgga tcttcaactc gtgcgcgagc
    37441 cgcttcaccc gcttgggccc gagcccagga atgcggagca actccgtgag agtcgccggg
    37501 acccttttgc gctgagcctc tatggcggcc gcggttcctg ttgcgagaat ttctttgatc
    37561 tttcccgcca gatcgtcgcc gatacccgga aggctggtca gatcgtcgcc cttcgccacc
    37621 atctccccca cctcggcagg aagatcgcga agcgttctgg cagcgaaccg ataggcccgg
    37681 acccggaagg gattggcgcc ttcgatctcc agcaagtcag ccatttcctc gaaagctgtc
    37741 gcaacctcgg cgttatgcac tgtcataaac gctccgctgc tcgtgcagga ctgcctgcat
    37801 ccatagactc ctcactgacg acccgctgct tccggccatc gccataacca atgattctta
    37861 agcaatgggc gggccaccca ccgtcggcat ataggagcga gcctcgcaag agcaacgtcg
    37921 cgttcttccc cagtcacaaa atcagggctg tcgcagaacg cctccacggg ggtgtcctcc
    37981 ccagcgccca acgaaccgaa caatcgcccg gccgtctcct cccacacgct tgaatccctg
    38041 tagcgtctct tgtgaaaagt ctcgcaggtt tcaaccggga gacgagcggc gcgagacgaa
    38101 cgacgaatgc gcggcatcag gcttgctgat cacaggagag ggtattgtga aacgctggac
    38161 caatggaggc aaggcaggat ggctggatgg atcgatcgct ggcgggctgc aacggtggca
    38221 atcgggacaa ttcaagacgc cgacgtacgg tctcgcaccg gccgggtttc aaccaagaaa
    38281 ttcttcgccg tggtcggtac cggtgtgctc ttctcgttgc acggcccccg cagacaggat
    38341 tgctggttgg tcaccgccag acatgtgttt ctcgacccga ctgagaactg gaaaccctcc
    38401 acgctgggca tcgtcttccc gcacattcat cgccggggcg tgaaacaggg ggtggagatt
    38461 ccgattcaac tcaccaaggc cggacggcgc tgctggtatc ctcatccgga caaggccgtc
    38521 gatctcgcct gccttccgct gcccttgacc atgcgacaac cgaaaccggg cggcccctcc
    38581 tccatcgcct ggatggacat tgccacgaca gcggacctct atgagggcgt ccccattatg
    38641 gtgctcggct atccggcggc gttcgatatt cccgattcac cgcgcgcaat tgtccgccag
    38701 ggcatcgtct cctgggtctc gcccgcccgc ccgggctcag aggtctttct catcgatagc
    38761 cacgtgtttc ccggcaacag cgggggaccg gtcttccggc ttcccaccgc catggatcgg
    38821 gaggggcatc tcaccgcggg aggggcggtg accctgctcg gcatcgtgac ccaggccagg
    38881 attcagagct tgcccctgct ggccgggggc aaacaggtcg acttgtacgt cgaaggcaag
    38941 aaaaactccg aacccctgct cacgcccagt tttctcggtc tcgggctggt tgagccggcc
    39001 tatcgcatca agcaactcct ggtctccgcg actcgccgcc atcgacgacg aaagcggccg
    39061 gtctcataac ggtgcggatc gactagttca tcccggcttt tgcgcccttg gccaaaagct
    39121 gccgcacccc ttcaggcgta cgcatcttcc ggcgatgttc gaggatgagc ggatcgatgt
    39181 gttccccgca cattacgcaa cgcataccga tctggttcac ctcacgaatc tcctcccgaa
    39241 ccatcaagcc tccgcagcga tggcatcgat cctgagcaat ctgtgtcggg tgcgtcacca
    39301 tatatctctc cttctcgttt gttcgttgaa gcgtccttcc atctcctgtt gtctattgga
    39361 gtgtggcgcc tcgaaaagga ttcatgattg tttcgccctg ccgatgatgc cctgcaagac
    39421 atccgccagc gtcgggcgcc cccggtcctc cacgtcataa ctcacccgca ccgtcggctt
    39481 cgtaatccga atgagtcgcc gtaaatccat cggtgtggcg atgaccacga ggtcgcactc
    39541 cacccgttca atcgtttcct ccagttcgcg gatctgctca ggcccgtacc ccatcgcggg
    39601 aaggagcggc cccagatgcg gatactgctg gaaggtgcga ttgagactcc ccgctgcata
    39661 gggcctcgga tccaccaggc aaccggcctc ataccgccgg gccacgagac aaccggcacc
    39721 gaaggccatg ccaccatgcg tcaatgtagg gccgtcttcg acgacgagca cacgcctgcc
    39781 ggtaatcctg tccggatgat cgactttagg cggcatggcg gtctccacga gcgccgcccg
    39841 gggattcacg gtttcaatgg atcgacgcgc cgcagccacc tgttcgggcg tcgcggtatc
    39901 cagtttggtc agcaccacca catccgcccg gatgagattc acctcccccg ggaaatacgg
    39961 cggctccccg gcccgatgcg gatcgacaag caccagttcg aggtcgggca tgaagaacgg
    40021 ccagtcgttg ttgcccccgt cccatacaat gacatcggcc tgctcttcga cctcccggag
    40081 aatccgttcg taatccactc ccgcatagac cgtcccaccc tgcgtgagat ggggttcgta
    40141 ctcctcccgc tcttcaatgg tgcaggccgc cgcgtccaag tcctccagag cagcaaaccg
    40201 ctgtaccgcc tgttgctcaa gattgccgta gggcatcgga tgtcgcaccg cggcaacccg
    40261 cagcccttcc cctttcagaa tcgagacgac ccggcgggcc acaggactct tgccggcccc
    40321 cgtgcgagtc gcgcagatcg acaccaccgg cttcttggcc gggagcatcg tggactgcgg
    40381 gcctgccagc cagaagtccg ctcctgccgc catcaccagg gaggcttggt gcatgagcgc
    40441 ttcatgggag acatcactgt acgaaaagac gacccgctca acatgctggg tcctgatcaa
    40501 gcgatcgaga tcctgttccg catggatcgg gatcccggaa ggatacaagg cgccggccaa
    40561 cgccggggga tagagacggt catcaatgtt tggaatctgc gcggcggtga accccacgac
    40621 ccgatagtca ggattatcgc gaaacagcac gttgaagtta tggaaatctc gcccggcggc
    40681 tcccatgatc aataccctga tctcgtgtgg cttggcgcga ttatccacga ccctcctctc
    40741 accagtggtg aataatgcta caggtggagc atcggctgtc ctcgcctgcg gcgcactaac
    40801 caccgtgatt tccctcgctt tccgctccgg ccttcacggg tttcaatggt ctacgccttg
    40861 cagaacgcat gctcggagtc tgtccgagga gtgtgaaccg cacaatgccg tggaacccca
    40921 tcatcaaatc gaggcgagac tgggagacga tcccgaatct ccacgattac gagtcggtgc
    40981 gaaaagactt ttcatgggag caggcgcgcg ccgaactgga tggcctgccc gatgggcagg
    41041 gactgaacat cgcccatgaa gcggtggtgc ggcatgcaac cggcccgctc ggacaacaga
    41101 ccgccatccg ttggctgggg aaaagcgggc aggtcgagga ctattcttat gcgcgcctcg
    41161 acgacctcac caatcgtttt gccaatacac tgcaggggct cggcgtcgtg aaaggcgacc
    41221 gggtgttcgt cctcgccggt cgcattccag aactctacat cacggcactc ggcacgttga
    41281 aacatcgcgc cctcttctgc ccgctcttct cagccttcgg gccggaaccc attcgcgccc
    41341 ggcttacgat cggccaggcc aaggtgctgg tcaccaccga atcgctctat cagcgcaagg
    41401 tggccggcat ccgggagacg ctgccccatc ttgagcatgt cctgctcatc ggcgaggaag
    41461 gacgcccgac cgccatcccc gggacgcaga acttccatca gttgctcgaa cagcacccgg
    41521 gtatctatcg aatcggcccc accgatccgg aagatgcggc cctcctgcat ttcaccagcg
    41581 gcaccaccgg gacacccaag ggagcgctcc acgtacatgg cgccgtggtg gcccaccaca
    41641 tcacgggcaa actcgcgctc gatttccatc cggcggacat cttctggtgc accgccgacc
    41701 ctggctgggt gaccggcacg tcctacggca tcatcgcgcc gctgacgaat gggctcacct
    41761 gcattgtgga cgaagcggat ttcgaggccg aacgttggta tgggatcctg caggaccagc
    41821 gggtctccgt ctggtataca gcgccgacgg ccatccgcat gatgatgaaa gtcggcgtcg
    41881 atctcgtgcg gaagtacgac ctgcgccaat tgcgttttct cgccagcgtg ggggaacccc
    41941 tcaatcctga agccgtcgtc tggggagaaa aggccttcgg ccgcccgttc cacgacaact
    42001 ggtggcagac cgaaaccggc ggcatcatga tcgccaacta tgcggcgatg gacgtccggc
    42061 ccggttcgat gggacgaccg ctgccgggga tcgaagccgg tatcgtgaaa aagaccgagt
    42121 ccggcggtct ggaggtactg aacgaaccgg atgtgcaggg agaattggcg ctacgccccg
    42181 gctggccctc gatgtttcgc ggctattgga atgagccgga acggtacaag aagtgcttcg
    42241 ccggcggctg gtacctgacc ggcgatgtgg ccaagaaaga tgccgacggg tatttctggt
    42301 tcgtgggccg ggcggatgac gtcatcaaga cctcaggcca tctcatcggt ccgttcgaag
    42361 tcgaaagcgt cttgatcgaa cataaggcgg tggcagaagc cgcggtgatc ggtaagccgg
    42421 accccgtcgc catggagatc gtgaaggcgt ttgtgtcgtt gaaagacggg catgagccaa
    42481 gtgaagcgtt gcggcgtgaa ttgcttggct tcgcgcgggc tcgcctgggc gcagccgtgg
    42541 cgccgaaaga gatcgccttt ctcccgaccc tgccgaaaac ccgaagcgga aaaatcatgc
    42601 gacgcctgct gaaggcgcgc gaacttgggt tgccggaagg cgatacctca acgttggaag
    42661 cgtagcaacg atggcggaag gcctctcagt agcagagcga ctgtcgggac acagataggc
    42721 gtgcgtggct gagcaaccag gaacgaggcg gcatggaatt taaggattac tacaaaaccc
    42781 tgggcgttga acgcacggcg acagccgacg agatcaaaag cgcctatcgc aagcttgcgc
    42841 gcaagcacca cccggatgtg aacaaagaga ccggagcgga agcacgcttc aaggaaatcg
    42901 gcgaggcgta cgaggtgctc aaggacacgg aaaaacgcgc cgcctatgac gaactcggca
    42961 atcgctggcg ggaggggcag gaattcacgc cgccgcccga atggggtgca ggattcgaat
    43021 tcacccaggg cggagcttcc gcaacagaag cggccgactt cagcgacttc ttctcgtccc
    43081 tattcggcaa cttctcccgc caagccgaat ccacgcatgc tcgtggtgaa gaccatcatg
    43141 ccaaaatctt cattccgctc gaggaggcgt ttcacggcgg gacgcagaca ctcaccctgc
    43201 gtgctccgca ggtgaatgcg caaggtcggc cggagttgcg ggagcgatcg ctcaccgtac
    43261 atatccccaa aggggttcga gaaggccagc acatacggct ggccgggcaa ggtgctccgg
    43321 ggatcaaggg gacctctccc g
//
LOCUS       DAORRQ010000038        42863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_59_length_42863_cov_112.613694, whole genome shotgun sequence.
ACCESSION   DAORRQ010000038 DAORRQ010000000
VERSION     DAORRQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 42863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42863
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59_length_42863_cov_112.613694"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PK224_12780"
     CDS             <1..463
                     /locus_tag="PK224_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB16661.1"
                     /translation="DIEMPEIDGLEACQRIRMEDQLQNLPIIVITAHTEAKDIQAAYT
                     CGATDYIRKPVIPAELIARTANALSLKQEIDARKLREHELLERTKELDHAFEQITTLH
                     GTVHICAKCKRVKSDGPHWQRVEDYLRRQARAKVAETVCDTCLHQAYPHLK"
     gene            complement(475..1164)
                     /locus_tag="PK224_12785"
     CDS             complement(475..1164)
                     /locus_tag="PK224_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16662.1"
                     /translation="MIRFDRGLRLLMTLLLLGSLSPVSTRAEESAGVRPLRNGLAVAL
                     ASLPAQTHLLLDQQSIEQFLVELDGAPPDWATVYGQGHHDPGHDERLFALNRERDARR
                     EGKAPLEWLIAFVWLGELSGFDEQEGGFRVVLGPKFNRTAWGEVRFKHEDLPSTLIAL
                     AGQGTARLRAWIKRGKPVPVAVLITGRLIPEESLIYDFSHEAEGLGLIMPVVRVEAVD
                     FVLEGGGETQP"
     gene            complement(1169..1486)
                     /locus_tag="PK224_12790"
     CDS             complement(1169..1486)
                     /locus_tag="PK224_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16663.1"
                     /translation="MDEQEEQVRRAIGTLLQSDPLIKLLQEVRLGRMKATDPGLRAVT
                     ESWIGVYAQVLKSQPVPAASLPRLDPAPRLQVLVDMGVLSWDHPGTKDLRDLFQRVSV
                     PAA"
     gene            complement(1518..1745)
                     /locus_tag="PK224_12795"
     CDS             complement(1518..1745)
                     /locus_tag="PK224_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRB16664.1"
                     /translation="MPIFEYVCKECNHRFELLVRGDVVPACPACKGTGLDKQFSAFGV
                     GATGGWPVTSSSGGGCGSCGDPRGPGSCSMN"
     gene            1827..2081
                     /locus_tag="PK224_12800"
     CDS             1827..2081
                     /locus_tag="PK224_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16665.1"
                     /translation="MWSEDTSFVFRISLEAQFPDDYEGPHDEHAWLREWELQIKPVLI
                     KNLFETLREYPAWKAHVRNRGKSPTEEIEVAMTRDFSPAP"
     gene            2096..3247
                     /gene="hemW"
                     /locus_tag="PK224_12805"
     CDS             2096..3247
                     /gene="hemW"
                     /locus_tag="PK224_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRB16666.1"
                     /translation="MRQATGRGLYIHVPFCHQRCHFCAFYLEIHHARAAEEFLGSLTT
                     ELRLYGEQRLFGSEPLQSVYFGGGTPTTLSAPQLATVLSAARETFGLQDEAEITVEAH
                     PGSVTRDSLSHLRRAGFTRVSFGAESMNQLELDRVGRPGSPMSTVQMATAARKAGFTD
                     LNLDLMYGLPGQTIESWEASLRQIVALAPTHLSCYALTVEEGTALQAAIRRGTVPAPD
                     ETLQNEMEDLAEDVLSRAGFTRYEISNYCRPGFASRHNQLHWTGGDYLGLGPSAQSYV
                     AGRRFGNVSDLSAYNASLRTGTLPVTASEQLTTADDACERLVFGLRLTEGITLGETGA
                     LAFSGLTGKVNELIGDDILERRGERVRLTARGRRYADTVAVALLASLES"
     gene            3317..3514
                     /locus_tag="PK224_12810"
     CDS             3317..3514
                     /locus_tag="PK224_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16667.1"
                     /translation="MPVNMDPTKRRRRQSAEQAAAAADQDSSQIKRFGEDTEADREKA
                     IAEAELKNLWDATEVIDMDTW"
     gene            3614..4330
                     /locus_tag="PK224_12815"
     CDS             3614..4330
                     /locus_tag="PK224_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16668.1"
                     /translation="MNALSPRNALFHPFHLCAPHTLEALLARYDAVHFRDYMALRLTP
                     LMGTTAYQDRMGDDHPMLVTSGRLVQGYPVSGPLDDTAVTAIDRDLSDSRWRTLFHDG
                     LRNDRRFQRGLFDLTHAMRIGSSLVPGPAALLRLLEPNRAAALYNVALVQRLAKPTLT
                     LDEAYQFEYGLALLKTAAAQVYTIRLSRAHNLVPVTDSHTHHVLLSRTLAREGIDLAH
                     ESIGASVGAPLSQHTSGLHE"
     gene            4445..4738
                     /locus_tag="PK224_12820"
     CDS             4445..4738
                     /locus_tag="PK224_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRB16669.1"
                     /translation="MSTLTPAMTPEALDTTDVGTGDDLEARVIVFNCDCHTYQQVIAL
                     FCTCIPGMNSSRAFELAWRIDHEGQATVYSGERKAAEEIGKKLAAGGLRVGVQ"
     gene            complement(4788..5135)
                     /locus_tag="PK224_12825"
     CDS             complement(4788..5135)
                     /locus_tag="PK224_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16670.1"
                     /translation="MERRAASHTEEEEMNQRRRLLGLARKGDPKAISKLFELYQVRVL
                     NGDMLSKLNKSYYKAAAAQEQKASQASSKPAKMAKGAHGSAKSSPKPSTSQPVAASKP
                     KTTKATGAKKPAK"
     gene            5383..6060
                     /locus_tag="PK224_12830"
     CDS             5383..6060
                     /locus_tag="PK224_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 domain-containing protein"
                     /protein_id="HRB16671.1"
                     /translation="MEHAAAIDTQTTVADDPQARALLQRAFESTARWQPDFRGFSADL
                     TVNTNGQQVRGTVVVKGPREVTVQLPDETIQKWAQEQIGMIAVHRAPRKFEESDGKHR
                     LTMEAGEDHPLGRRLDIHGDGMQSFYRIKDNRITQINRKMPHVAFTINVEESSTTQDQ
                     KQLTTKYTVYYFAPQDAKLRNVESFTDTHVRVGNSDLPATRRIISYENGAVLVRTLTF
                     TNHKLLA"
     gene            6060..6491
                     /locus_tag="PK224_12835"
     CDS             6060..6491
                     /locus_tag="PK224_12835"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRB16672.1"
                     /translation="MDLRTTFPRSMRVRLEGYVHLARMIDKCRAVLAGTEGEYIYPCP
                     MDDRLMAFAGITAAQFTAAVKQHLSDEGVIQWFRGIATPHAQAELEEWNRMMLSRGPT
                     TPEKRASFNKYRDAVDPSRTDLTSWAELQDLEEGRTVPRRA"
     gene            6566..7123
                     /locus_tag="PK224_12840"
     CDS             6566..7123
                     /locus_tag="PK224_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16673.1"
                     /translation="MALLQQDLVPASGDSLITFDTDTNLEWLSLTKTVNLSISDVRNG
                     AGGYATTYGFRYANGAELQALWNHAGITRFAPTQPVPVPDSNSAGIQKLIDWMGGTTS
                     YPTTGTIQTQGIFMVPPAPGYPGVGQLWFFTNNPGGSYATTDIFPNVPQGVTPEDYRS
                     SALASYLVRNHVSPAPPRNLRVGEK"
     gene            7575..8171
                     /locus_tag="PK224_12845"
     CDS             7575..8171
                     /locus_tag="PK224_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16674.1"
                     /translation="MALVQQNLVPATGDHLVTLDTETNLEWLSLNATANLSYLEVLGG
                     AGGYTTTYGFRYATGQEIGLLWQHAGITKYGVTHVVPFPQSNHVAMETLIELMGGATL
                     YPSVTSGSVLVQTQGMMKFRGAGVTTPITPMSVGQLWLFKNNPGGSYADTNPAGNAGK
                     RTPEIASYLVRDRIMPAGSISRGKSAKAVKARSAKKQG"
     gene            8498..9055
                     /locus_tag="PK224_12850"
     CDS             8498..9055
                     /locus_tag="PK224_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB16675.1"
                     /translation="MTTKTTGDQPLFTPEQTFSSHTSAKNILIAVDDSDESARALHYV
                     GVLLRDIQDVNVTLFHVLNPMPRELMEHGGSENPETEHHLGEQLRKDQEEWIRTEGAI
                     EYPILVTAMERLGQTGFPLDHVTLKLGHERDIADSIMDEARAGGYGTVVVTRHGPTGT
                     KRLFSSRITDRLVRDLSGVALWVLG"
     gene            9118..9864
                     /locus_tag="PK224_12855"
     CDS             9118..9864
                     /locus_tag="PK224_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRB16676.1"
                     /translation="MNCTSCETALPLRARFCPACGVRVTLPEDPAFDEAALQAAGCGD
                     ELNQRTAPAPLDHEALKARLIDCYVNFSMARELSGWLQALGRPSTGTMQEKLTRLRQQ
                     ADSLVLPAESLPRQTIWYLNRYDEKILTEICEELGIDNSGPKERQITRIYHAVGLREG
                     WLQPLSEDARNIITETFLPILRAVDHRNYADLDQWGELGDLLDRESRTRLDHQGHGSA
                     FMAVLIPSLLQEAHRDLLQHELDARAGKPF"
     gene            complement(9946..10368)
                     /locus_tag="PK224_12860"
     CDS             complement(9946..10368)
                     /locus_tag="PK224_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB16677.1"
                     /translation="MAWNIASKIVHRVVTIDENHTVLDAATLMAEEFVGSALITSSSK
                     ITGVFTERDLMMRVVGRKRDPENVKIKDVMTKNMVTVSPKDTANHCLNLMKEHRCRHL
                     LVFDNEEFIGIVSLRDMVALLMEEKEALIAYLHQYISS"
     gene            complement(10475..11332)
                     /locus_tag="PK224_12865"
     CDS             complement(10475..11332)
                     /locus_tag="PK224_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB16678.1"
                     /translation="MRTVVAVDWSEQSFNAVKVVCRLFTHESLTLIHAVDLRPFENPL
                     FAQPLGSKSAGALREGMVAAGERLLDQTGALVPVTIPSIQRVYNIGNPAPVVLEAIRS
                     AHADLVAVGSRGRGRLAELVLGSVSHRVVLHAPCSALLVRDDPGSIRRVLLTVEGYED
                     SVRLRSWLSTHRFSRPIELSLLTVVPTIPIGDSTMEFRYDKWTAEMDQYARQLVDETA
                     AALRTMYPAVTAQVEHGDPAHCITKAAGHADLVIVGSHGWRGLEHFLLGSVSHAVLHK
                     VTCPILVIR"
     gene            11424..11546
                     /locus_tag="PK224_12870"
     CDS             11424..11546
                     /locus_tag="PK224_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16679.1"
                     /translation="MRHHTPYPAMEVKRSENIWLVGLGTELANGEKPIEYRSVQ"
     gene            complement(11624..11980)
                     /locus_tag="PK224_12875"
     CDS             complement(11624..11980)
                     /locus_tag="PK224_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16680.1"
                     /translation="MIVEVESNDNCDTSFFARFMEVGPARPVVQVRLYERNPTGEWCW
                     VTGWSDNEQAPTCPAYVQLIEDSGSGLAYLVYGGLYGLRFRPIQFDEPWSLQSSHQWG
                     EAYLSLADVRDVRYAG"
     gene            12277..12663
                     /locus_tag="PK224_12880"
     CDS             12277..12663
                     /locus_tag="PK224_12880"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRB16681.1"
                     /translation="MTSENVPAVVARYFSAIGEMDPDAWAACFAEQGTSYEPGSPTPL
                     QGHAALRQFLAGVLGAFEKITMTQDHVFLSGNRVAVKFTGRGTGKNGRQVTFEGIDVF
                     EINQEGKVQTMWGYWNPAALMAQLQG"
     gene            complement(13090..13716)
                     /locus_tag="PK224_12885"
     CDS             complement(13090..13716)
                     /locus_tag="PK224_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16682.1"
                     /translation="MTAGEASVRLQRIYAALDAVIDNDINKALPVLISSTQGRGVFQD
                     FRGSLSDAELENLAHSVIHNIANLRDHTRSWIVKSAKGVNKQQVDEFLKANESVAVIQ
                     DLSNNDKHGYPPRNGGFSGKAPRLTNLRRVMRLTTRAGPEGSVAFSIAPSGEQRVAGT
                     GSANLIVTADVLNSDGTSIGDLYTIQLKAIEAWEQFLRELGVFSCGER"
     gene            complement(13759..14619)
                     /locus_tag="PK224_12890"
     CDS             complement(13759..14619)
                     /locus_tag="PK224_12890"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRB16683.1"
                     /translation="MFVQKVLVATLFTLLFLLASHQEYLRSEGFNPELTVSAQFNFPA
                     HEPTIEINGKDGGAMVLVPAGEFIWGGSDQIPRPPSRIYLDAFYLDKFEVTVSRYSKF
                     LANSDRRHPNRWSESNLGIDADRPVIGVTWYDAHEYCSWADKRLPTDAEWEKAARGTD
                     GREYPWGQEVPTQKHANFDKRDDWWKGYSTLAKVGSYALDISPYGIYDLAGNAEEWVA
                     DWIDIEGQYYKTDISRNPAGPVLGQFKVLRGGSWFTPATGLPIRIPSWSDPSLTNFSI
                     GFRCAQDFTK"
     gene            complement(14991..15728)
                     /locus_tag="PK224_12895"
     CDS             complement(14991..15728)
                     /locus_tag="PK224_12895"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRB16684.1"
                     /translation="MNRRFSITICSIVGLGLLLLLVAVPVGFAREPVPSYDPQGYVSD
                     HAGVIDAEWRARIRSVCQDLERKTGVEMVVVTVPTLKPYGSANDYAVGLYQKWGIGSA
                     QNEHGVLVLLSVQERQAAVTMGRRMLPVMGGNVVGKVGHEYLDPAVKNGHFGEGLYRT
                     VVGLASVSQEIRVGSIKKTHFRGLGFWITIFTVGGALWFLWWLSRPDLRHPYGRLRRG
                     EYWGSGQGGFGGNFGGHGGGMSGEGWK"
     gene            complement(15725..16483)
                     /locus_tag="PK224_12900"
     CDS             complement(15725..16483)
                     /locus_tag="PK224_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16685.1"
                     /translation="MTQPTALDIPSEVQELLKRYVKETTTLLGSQLEGILLYGSAVGG
                     EFLPGRSNLNLLLMLTGYEREGLKRYAKAHKRWSREQFVVPLLVTEAELTRPGTVFPL
                     EYLEIQEQHRVLWGRDPFIGMHIDRTHLAYQVQQGIQGNLVRLRQRYIEGGGTEEAMT
                     MLLPLSLTALLPCLRGLQRIAGQPALFQSDALLAGVRTLTGLDLAGLNDVLELKRGII
                     SPGPVEVPRLFERYAANLDALIALIRMDGTVTPR"
     gene            complement(16670..17947)
                     /locus_tag="PK224_12905"
     CDS             complement(16670..17947)
                     /locus_tag="PK224_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRB16686.1"
                     /translation="MGRAMIETDVAIVGAGGGGAVLALMLAQQGVRSLVLERAAGPPQ
                     GLRGEILQPNGQRVLDRLGLLDKLPAHAVRSVRRFNFCRAGGERLCTVDYCELPAPYN
                     RALVTLPNVAHHAILDALEQQNPGALWYDATFTGLRFDGSRVIGLQATRHGEPVDISA
                     RLVVGADGALSKVRESLGITAQLHRYPQSYLIAILKAPPGFDEARYLVGKREILGLFP
                     AAGQQVYAFYMIKAGSYEAVKAQGLEALRRAWVRIDPGMEGMVQELVDWSQTGYMPTG
                     RVKTDRWVADGALLIGDAAHAMNPHASQGRMQAMVDAVVVADLIPGWLKKNDFSAESL
                     RAFEVARRPQVSMLQRLADEQCRFWNTGNPVVAYLRDRVFRTLDRNARLRYRVLSTTA
                     GLRSRPPFSLLDRVMAAGLLPDPRAQVRSSDEA"
     gene            complement(17960..18505)
                     /locus_tag="PK224_12910"
     CDS             complement(17960..18505)
                     /locus_tag="PK224_12910"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRB16687.1"
                     /translation="MSRLISSQLVVWTVVALICLTHVPVYASATIDVYYAPEDQPIDR
                     VVGLYAHATRYIYVSVYALTAPSVVKALVEAKRRGVDVRVITDRERLNDPKQHSAVNT
                     LRLAGVPIRINRHDALMHLKQVVIDDAINTSGSANHTTSGNRYNDERLDVITDARLTA
                     KAREKFLTMWNDHERFMTWTQ"
     gene            complement(18720..19097)
                     /locus_tag="PK224_12915"
     CDS             complement(18720..19097)
                     /locus_tag="PK224_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16688.1"
                     /translation="MWKKNFLFRAHEAAPLKESENELFHDAEPALDSAGLQMEKFLSV
                     WVQGEGEDDSPSMYTNIYVRTATLDFRTRAGFLQPLQGRSHQIKQMLTPEQKGFLREW
                     LSKASPQAWEESDDHFRTLFDLE"
     gene            19489..19689
                     /gene="thiS"
                     /locus_tag="PK224_12920"
     CDS             19489..19689
                     /gene="thiS"
                     /locus_tag="PK224_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRB16689.1"
                     /translation="MQVMINGKSEEIAGGSVLDLLKAKSIEPQMVAVEVNDTMLERTH
                     LETTQLKDGDHVEFLFYMGGGR"
     gene            19686..20615
                     /gene="cysK"
                     /locus_tag="PK224_12925"
     CDS             19686..20615
                     /gene="cysK"
                     /locus_tag="PK224_12925"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRB16690.1"
                     /translation="MTVKAHADITELIGGTPLVRLNRLSKPGGATIYAKVESFNPGGS
                     IKDRICLNMINEAERLGKLKPGGTIVEPTSGNTGIGLALVAAVRGYKLILVMPESMSM
                     ERASLLSSYGAQLVLTAAWEGMKGSIKEAESIVAQNPSYYMPDQFSNPANPDMHRKTT
                     GPEIVNALDGRVDAFVAAVGTGGTITGCGEVIRERNPAAKIIAVEPAGSPVLSGGDPG
                     PHKIQGIGAGFVPKVLNRTLLDRVVTVTDDEAYQTAKLLAKKEGLLVGISAGANVFAA
                     QKIAEELGPGKNVVTILCDTGERYISIEKYFNI"
     gene            20931..21758
                     /gene="moeB"
                     /locus_tag="PK224_12930"
     CDS             20931..21758
                     /gene="moeB"
                     /locus_tag="PK224_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRB16691.1"
                     /translation="MEFTETQINRYSRHILLPEVGGKGQKKIVQAKVLIVGAGGLGSP
                     AALYLAAAGIGTIGLIDSDVVDLSNLQRQVIHQTPDVGRPKVVSGKEKIQALNPDVNV
                     VMYEERLTAGNALKIMSGYDVVIDGVDNFPTKFLINDACFFAGKPLVHGGILRFDGRV
                     TTIIPKKSACYRCVFKKPPPEGLVASCQEAGVIGVLAGIIGTIQATEALKLILGIGRP
                     LTDRLLDFDARRTQFREIRIKRNPDCPLCGERPTITELIEDGDVAGPTCALPGQRNL"
     gene            21772..23022
                     /gene="thrC"
                     /locus_tag="PK224_12935"
     CDS             21772..23022
                     /gene="thrC"
                     /locus_tag="PK224_12935"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRB16692.1"
                     /translation="MSKMKALVCRECGKEYPPKAIHVCEMCFGPLEVKYNYDEIKSTI
                     SRKKIEQGPNSMWRYIDLLPVESTAIIGPHAGLTPLVRAKNLGAYLGIDELYIKNDTV
                     NHPTLSFKDRVVSVALTRARELGFETVACASTGNLANSVAAHAAAAGMKCYVFIPADL
                     EAAKVLGNLIYKPNVVEVEGNYDDVNRLCSEIAGEHGWAFVNINIRPYYAEGSKTLAF
                     ETVEQLGWRTPDQAVIPMASGSLLTKIWKGLHEMHALGLVDSVRTKINGAQAEGCSPI
                     ATAFKAGRDFFKPVKPKTIAKSLAIGNPADGYYALKATAESKGAMDAVTDEEVVEGIK
                     LLAQTEGIFAETAGGVTIGVLCKLVKQGLIKKSDVTVAYITGNGLKTQEAVVDAVGRP
                     FRIQPSLVNFQKTFKMGKNSGGDS"
     gene            23019..23291
                     /locus_tag="PK224_12940"
     CDS             23019..23291
                     /locus_tag="PK224_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRB16693.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVESAAANIVDMIGSLDATYPGLKNR
                     LCDEKGDLRRFVNIYVNEEDIRFLNGKETSLKDGDEVSIVPAIAGG"
     gene            23294..23533
                     /locus_tag="PK224_12945"
     CDS             23294..23533
                     /locus_tag="PK224_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HRB16694.1"
                     /translation="MTSLRFHIRFPENKIKEPIIYQIGHEFKVVTNVRRADVRETTGW
                     MDLELTGETEEIERAIDGIRTKGCVVDPIELNVVE"
     gene            23619..24422
                     /gene="moeB"
                     /locus_tag="PK224_12950"
     CDS             23619..24422
                     /gene="moeB"
                     /locus_tag="PK224_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRB16695.1"
                     /translation="MEFTEQQIQRYSRHIILNEVGGKGQVKLSKAKVLLIGAGGLGSP
                     AALYLAAAGIGTIGLVDGDVVDLSNLQRQILHTTATIGVPKVESGRRMLSAINPDITI
                     KTYQLNVDTENILGLVSDYDIVLDGSDNFTTRFLVNDACFFARKTLISASMFRFEGQL
                     TTIKPHAGYPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TIADKLVIYDALEMKFRKVSRPKDPRCPLCSATPTIKDLGGDYSVACTI"
     gene            24424..24936
                     /locus_tag="PK224_12955"
     CDS             24424..24936
                     /locus_tag="PK224_12955"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRB16696.1"
                     /translation="MPDLSIPQHILDQIVAHARELAPFECCGLLAGTNKTVTHLYRIT
                     NIVAVEGAEKLSTFDDDKVAHLERLSPEERAEIAFVMDMGDFSAAKKDIRKNGLDLQV
                     VYHSHPKDPARPSHTDIKIATDYEEIWERINLPVPAYLLVSLMHSPAADIRTYWIADG
                     KVRPADVHIM"
     gene            25037..25366
                     /locus_tag="PK224_12960"
     CDS             25037..25366
                     /locus_tag="PK224_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16697.1"
                     /translation="MGFGLLIVALILCLTGVTNPEHTSAAEKSYYSPIINVDVDNSRI
                     LISTLGAVFWVEVPEEAKAHIEKLPQSGLVDIVVETREGQPPLLKTWKVKSGESTCLH
                     FDGKVCK"
     gene            complement(25469..25678)
                     /locus_tag="PK224_12965"
     CDS             complement(25469..25678)
                     /locus_tag="PK224_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16698.1"
                     /translation="MAGSTGRKTVSVSRGLMMLCDVCYEQVFAENLVDQRNFVSDHES
                     LFDTICMGCTDINRPLVDDMLGSSE"
     gene            complement(25724..25918)
                     /locus_tag="PK224_12970"
     CDS             complement(25724..25918)
                     /locus_tag="PK224_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16699.1"
                     /translation="MVLIYESDPLDNEDARGHFYHVCRGRIDRTEIRLPEGESIYECA
                     VCGVDLEPEDFWIARQKGSV"
     gene            complement(26148..27872)
                     /locus_tag="PK224_12975"
     CDS             complement(26148..27872)
                     /locus_tag="PK224_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB16700.1"
                     /translation="MSSLLRVLSYLKPFRMLAVVTFVCAGLATALELVPPYLVKIVID
                     DVIQSKRPEMLPWVLGALLGSYVLRNLMSSMRVRFNNLLEQKAVHALRMQVFGALQRL
                     SLSYFENRSTGEIMTRVTSDTEHVERIFVDGLEGLLTASLTLVGITIMMFFLNWKLAL
                     LSLLPIPILIVCAAWFTRRVHGYYAEIRKSVADLNAYLQDALSGIKETIGFNQHEYER
                     ERFETLSQTYSQNSLRAMFLWSWYWPGMVFVGSTGTVLILWYGAGEVLAGELTVGQLV
                     MFLSYLGLFYTPINEIHSLNHMLQHALAASERVFEILDTKPEVEDRPGAVRPVERLRG
                     AVEYRHVGFGYRKDGTVLSDVNLTVRPGERIALVGPSGAGKTSLLKLLMRFYDVRSGA
                     VLVDGHDVRELPLDFLRGQIGLVQQEPFLFNGTVRQNIVYSDLSAGHERVEAAARAAR
                     AHDFITRLPDGYDSWIGERGVKLSVGQKQRVSIARVLLKDPPIVIFDEATSNIDTETE
                     VKIREALNDLTKGRTTFIIAHRLATLHDVDRIIVVERGTIVEEGDHEALMKRGGVYAG
                     LYEAQFKI"
     gene            complement(27897..28427)
                     /gene="def"
                     /locus_tag="PK224_12980"
     CDS             complement(27897..28427)
                     /gene="def"
                     /locus_tag="PK224_12980"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRB16701.1"
                     /translation="MAILAISKLGNPILRQKALPLTQAEIKKAAFQQLIDDMFETMYD
                     EPGIGLAAPQVGRSQQLVVMDCPGEGGFPKTVLINPSIQFYGPEQVEGWEGCLSVDGL
                     RGKVTRPSTVRVTGLDRNGKPFDFEASGLYAVCIQHELDHLIGKLFIDRMTDFSTLTQ
                     MDEFQKYWQKEPASVI"
     gene            complement(28538..29341)
                     /locus_tag="PK224_12985"
     CDS             complement(28538..29341)
                     /locus_tag="PK224_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB16702.1"
                     /translation="MMGARQQMVGRLLVRGLVCLGLVGITVDQAWSDATVWPVQMPYE
                     APPKSEPVPDVSVAPNTKALTPEELQRAEALLPLLEGKQEFWAMGEFVHLGEPVLPVV
                     TKALAMPGPRIRYNAIETLSMIKSPAAVPALLDTAKLGTELPRIREHALRVAVRLDPM
                     QAAPAIEAMAKDANASIRKAAAVESRYVRRKEVIQPLIDLLGDDERFVALSAVQSLWT
                     LTRHETEFHDWDASNKQDRQAWAQEWIEWWNQSKETFEMPEPKSRKRAG"
     gene            complement(29393..32476)
                     /locus_tag="PK224_12990"
     CDS             complement(29393..32476)
                     /locus_tag="PK224_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRB16703.1"
                     /translation="MIVRIVELSLVQRFLICALGFSLLFGGLYAFQQLDIVAYPDPSP
                     PMVEVITQFPGWSAEEIERQITIPIEVALNGMPGLTDIRSLSIFGLSDVKIYFDFGTQ
                     YFFDRQEVINRLATVSLPQNVQPALSPWWAIAEIYRYELAGDGKTSLTDLKTIQDWQV
                     RREFRRVPGIIDVTAFGGTTQEYHVDIDPGKLISYQVSLAQVMEALTNSNANVGGNYL
                     TVGAQNYNIRGLGLINGLADIENVMVAEKEGTPIFVKTLGTVSVGHRVRLGKVGIDDN
                     DDVVEGVILLQRGYKALSVLERVREKVEELNKWKLPEGVKVKTFYDRTALIHTTVETV
                     TDILISGMVLVFVILFVFLGHFRASLIVALTIPMSLLFTFTMMVMIGQSANLISLGSI
                     DFGIIVDATLVMVESIFFHLGHDRRLGLTVPQQIVRAARQVGQPIFFSTAIIVVAFIP
                     LFTMTGVPGKIFAPMSITYGFALFGALLMAFTLAPVLCSLLLTGVVKEEDTRFVGAIR
                     RTYLAVLQWALRHNVKVIGAALLLLVGAFGALQFIGGEFMPALEEGNLWVRATMPVDI
                     SYDEAARLTGEIRQMFRQSPEVVTIVSQLGRPDDGTDPTSFFNAEFLANLKPQKEWRP
                     GLSKDQLVEEIEARLKVIPGIVFNFSQVIQDNVEEAMSGVKGENSIKLFGNDLKTMEA
                     KAVEVEAVMKGVRGVKDLGIFRLVGQPNLLIQVDREASARYGLRVSDVNAVVQAAVGG
                     QGVTQVYEGERLFDLVVRFLPEFRRDAETIGNILVNTPDGARIPLKQVATIRTQTGAF
                     IIYRENNERYIPIKFSVRDRDLESTVKEAQTKMAQAVSLPERYRLEWAGQYDQLTAEQ
                     KRLTMIVPVSLVIILFLLYTTFNSLTNALLVLVTVPFALIGGIFSLVFTGTHFSISAA
                     VGVISTLGVAILGGVLLISRIEDFRRHGLDLREAVLKGADVQMRPILMATLAAAIGLL
                     PAALATGIGSQAQQPLARVVVGGMLTAAVLILVVLPVLYEVVHRRTASTRAVEAE"
     gene            complement(32529..33674)
                     /locus_tag="PK224_12995"
     CDS             complement(32529..33674)
                     /locus_tag="PK224_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB16704.1"
                     /translation="MIIGVRSVAGVAVALSLWACSQTQEPAASKPPVTPEHVAVQPPP
                     QSIGQIETAVVDFKPIQPELVLVGKVAYGEDRYSKISSPLQGRVVEVRAKLGDRVEAG
                     ATLLVIDSSDITAAYSEFVKEASEMEYSTRAQELAKELYATKALALKDLKQAENDLIK
                     ARAEFRRAKERLLSLRIPAQELEKPLAQQRITSRFEMKSPLTGTVVERAVTPGQSVGS
                     DAGQVLFTVADLDRLQVVADVYERDLALVKVSQVGRINVEAYPGTDFASVVASIGDVV
                     DPNTRTIKVRAWVDNREQRLKPEMFARLRLDVGDATPFLTIPKEAVVEIDGKHFVYLV
                     DAPNHYEKREVVVSNVSSGQVRILEGLTSGQRIVIKGAVLVKGQEAK"
     gene            complement(33671..35017)
                     /locus_tag="PK224_13000"
     CDS             complement(33671..35017)
                     /locus_tag="PK224_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB16705.1"
                     /translation="MLHYRDCRRVGPCLSALVCRAWLAIGLLGFFVIGGAIAQAAEST
                     PPPTVPFSPPVVRLNLSEAMALFLRQNLDLLIAKYGIESSKGQQITARLFPNPVAQLG
                     NVASFTQGNTLSKTGALTMQVQQLFELAGKRGYRIESAGFGVQSAEADFEDAVRQLGF
                     TIKDAYYRVLVAQRRLSLAEENRDRFARILDVNTIRFKKGYIAEVDLIRIRLQVVDFQ
                     AQVIEAIQEGESARADLRQLLRLSPASRLELTTEMDYRRVDPDMGRLRSVALDVRPDI
                     KSRRAALLQREADLKLAKAFRVPDVTIGAGYAVQGSRGPDNQQMGILNLGVPLPLFNR
                     NQGGIVQAEVGVQSAQAELDRTVNQVENQVDVAYQNLLQSRRLVEAYLAGVLEDARST
                     FTIVERAYERGGATILDLLDAARTSRTIQQNFIEALFSYQRNLFQLESSVGQEITS"
     gene            complement(35115..36185)
                     /locus_tag="PK224_13005"
     CDS             complement(35115..36185)
                     /locus_tag="PK224_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRB16706.1"
                     /translation="MGTPLRRMSPWGLCLLWLAGALFSCGQGAGSDPAESRPVLHYFT
                     WSDYVGPDIIQEFERRERVKVVIDTFSSNEELLAKLQSGATGYDVAVPSDFMVAIMIQ
                     QGLLSELDQQALPNASFLEPHLQALPFDPERRYSIPYLWGSVGIGYDSAVIPTPPDSW
                     AILWDPRYKGRISMLNDQREVFGAVLRSMGQSMNSTDPQVIEAAKERLLRQKPLVKTY
                     TSDHYDQLLASGEVVLAHAWGGPVARAMAERPSIRYVVPKEGATLWADCLVVLRTAPQ
                     KQLAMRFINYLMEPQVAARTSERLRFASASRPARELVNASTRDNPAVYPPLDQLDRLE
                     WMKDVGRGIRLYDRAWTELKMQ"
     gene            complement(36186..36959)
                     /locus_tag="PK224_13010"
     CDS             complement(36186..36959)
                     /locus_tag="PK224_13010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB16707.1"
                     /translation="MRRGSTGLSLVSGLAMLFLYGPILVLVLYSFNAAHLSMAWRGTT
                     LKWYAALWHDEALLAATANSLLIAVISTIGATLLGGLMALGMERMPLRRQQIIEGGLV
                     LPLVIPEVMMGVALMLLFVMLKMPLGLTTVILGHIVFNIPLVTIMIRTRLRKLDPALR
                     EAAADLGADSWQVFRHVTLPLLRPAIWGAVLVAFTVSLDDFLVTFFTAGPGATTLPLK
                     VYSMIKSGVTPEINALSALLLLISMACVALSLHLQRREV"
     gene            complement(36956..37846)
                     /locus_tag="PK224_13015"
     CDS             complement(36956..37846)
                     /locus_tag="PK224_13015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB16708.1"
                     /translation="MPSHDLSSPPAPGQSTCRSCWLAAPGLLWMGLFFLVPLALVFAI
                     SFASRGTYGGIVWEFTAANYLDLWHPLYGKILGQSLWYASLTTAICFVLGFPLAYMIA
                     RSPARWQPVLLLLVMLPFWTNFLVRTYAWMIVLRQDGLVNGLLRALGIVDAPLELLYT
                     PTAVVIGLVYGYLPFMVLPLYVAVERLDPRLVEAAWDLYASRWAIFTRVVVPLTMPGI
                     VGGCVLVFIPSIGSFITPDLLGGARSMMIGNLIQHEYLVVRDWPLGSAVSFVLMAIVM
                     AAVALYYRHAARTAGPLEGR"
     gene            complement(37849..38988)
                     /locus_tag="PK224_13020"
     CDS             complement(37849..38988)
                     /locus_tag="PK224_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB16709.1"
                     /translation="MATSSIPPFTASEQSLSSTLPTIDIRGIVRRHGAVTAVDRVSFQ
                     VRRGEFFSILGPSGAGKTSVLRMLAGFDDPDEGDLLIDGQSMLGVPPNRRPVNLVFQS
                     YALFPHLTVFENVAFGLKMRRVSAALIAEQVRQALAMVKLLGKEGRMPNQLSGGEQQR
                     VALARALVNKPAVVLLDEPLAALDQQLRQAMQVELKSIQEQAGLTCVCVTHHQEEAMM
                     MSDRIAVMHQGRMWQVGSPREVYERPCNLFVSRFLGVSNELTGTIGASVGEDRVFRPD
                     DAEQAPLTVRTASTEQAGRSATLSLRPEHIRMAHERPSTSDPVLSGRIEKVLFAGSEM
                     KYLVRVAPGRFWETRMTSGTSLPAFAAGDPVFLHWSLADARLFFE"
     gene            complement(39028..39306)
                     /locus_tag="PK224_13025"
     CDS             complement(39028..39306)
                     /locus_tag="PK224_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16710.1"
                     /translation="MKKQNARQAVVKPDIEAANPMMEMVWRLERLERQVQRLSELVRN
                     HAEDNDRLVRIVAEDRDVIRRELLRKHEHRVRVRKHLRDVSSKVDLEH"
     gene            39442..40680
                     /gene="glgC"
                     /locus_tag="PK224_13030"
     CDS             39442..40680
                     /gene="glgC"
                     /locus_tag="PK224_13030"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRB16711.1"
                     /translation="MAKIFTMVLAGGKGERLNPLTEQRAKPAVPFGGKYRIIDFTLSN
                     CLNSGLRQMAVLIQYKSHSLDRHIRTGWNILNPELGEYIVSVPPQQRISEEWYRGTAD
                     AVYQNLFLLDNDHPEFLLVLAGDHIYKMNYADMYNLLIEKHADAVVGAIETPLADANR
                     FGVIAVDKDRRILSFDEKPEKPMHIPGDPTQAFVSMGIYLFRTDVVREQLIRDAKEGT
                     KHDFGKNIIPRMIKEQRVYAFKFQDENKKAVKYWRDIGTLDAYWEANMDLVAVDPLFN
                     LYDQQWPIRTYQGQFPPAKFVFAQDFQGGRMGVALDSIVCGGCIISGGRVQNSVLSPN
                     VRVHDHADVRESVVMENVVIGEQARIRRAIIDKDVIIPPKTVIGFDPVADRQRFKVTD
                     SGLVVISKGMKLHAAVDSSG"
     gene            40655..42517
                     /locus_tag="PK224_13035"
     CDS             40655..42517
                     /locus_tag="PK224_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRB16712.1"
                     /translation="MPPSIHPVDLVATLRQRNLTPAIIFLTSRRACDEAMQSFERSQV
                     TLPKTQQEQIARVLAQVTEQYPSIAEHPLIDTVQRIGVAAHHAGHLPSWKIAVEELMR
                     TGSLDAVFATTTLAAGVDFPARTVVVTQSSIRKARDFTDLTASEIQQLAGRAGRRGKD
                     LVGFAVITPSPYIDLTVLTKGLTGQPEAIHSQFVISYPMVLNLLKAHPREQIQSILAK
                     SFAQFQLNQRTAVLETKLDGLRVEMEPFGPRVCSDWITQWQVFDQARKRKKPRGLSAR
                     QESPDVAARLHFLTPGRVVGLIRGRGIILRQYRSKGQHAPMVTLLRDGGSLNECPASI
                     VTQVFDRTFDCEETSAYPWCTPQTLEQLTHHLLDLPPRLPILPILTHQHEEIPLDSSI
                     IQTLGDFACPTCPSRQACQRDFPRASRVRQEIQRHMKSIQALQTSLWHRFQERIDVLE
                     RFGYLNATAQLTADGEWARLIRIDHSLLITELIRAEAFGAIDPPLLAAVMASIAHDDD
                     RPGSFPRGSAGLVTLLFQVRKLAESLVPHEAPPMLRADVAALTERWVADQTLTWIGLA
                     RLTTMAEGDMFRLFARTIEFLSQLKTLKATHPSLAESADRAIAGMRRGVLEELP"
     gene            42600..>42863
                     /locus_tag="PK224_13040"
     CDS             42600..>42863
                     /locus_tag="PK224_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16713.1"
                     /translation="MTTHELEQLLLQQPVSLLHRLARGRISRHFRSGKRKLIDLLLQA
                     SAENRPGLESDLAALIEEQSHRQQAVPVEQAPKPKVHPRPAAAS"
BASE COUNT         9459 a        13488 c        11785 g         8131 t
ORIGIN      
        1 ggatatcgaa atgcccgaga tcgatggatt ggaggcttgc caacgcatcc ggatggagga
       61 ccagctgcaa aacctgccca tcatcgtcat cacggcacac acggaggcca aagatattca
      121 ggctgcctat acgtgcggcg ccacggacta tattcggaaa ccggtgatcc cggctgagct
      181 gatcgcccgg acggccaatg cgctcagcct gaaacaggag atcgatgcca ggaaacttcg
      241 tgagcacgaa ctcctggaac ggaccaagga actcgaccat gcgttcgagc agatcaccac
      301 gctccatggt acggtccaca tctgcgccaa gtgcaaacgc gtcaaaagcg acggcccgca
      361 ttggcaacgc gtggaagatt atctacgccg gcaggcccgc gcgaaggtcg ccgaaaccgt
      421 atgcgacacc tgtctgcatc aggcctatcc acacctgaaa taagtacgtc ccggtcaggg
      481 ctgcgtttcc cctccgccct ccagcacaaa gtcgaccgcc tctacacgca ctaccggcat
      541 gatgagcccc agcccctctg cttcatggga gaagtcgtaa atcaacgact cctccggaat
      601 cagccgaccg gtgatcagta ccgcaacagg gacaggcttt cctcgcttga tccacgctct
      661 gagcctggcg gtcccctgcc cggccaacgc aatgagcgtg gacggcaggt cctcatgctt
      721 gaaccgcacc tcaccccatg cggttcggtt gaacttcggc cccagtacga cgcggaatcc
      781 gccctcctgc tcatcgaacc cggacagttc gccgagccag acaaacgcaa tcagccactc
      841 tagcggcgcc ttcccttcac gccgggcatc gcgctcgcgg ttgagcgcaa agagccgttc
      901 atcatggccc gggtcatggt gcccctgccc atagacagtg gcccaatcgg gcggcgcgcc
      961 gtccagctcc acaagaaact gttcaatcga ttgctgatcg agaaggagat gggtctgagc
     1021 gggaaggctc gccagggcga cagccaggcc gttgcgtaac ggcctgacac cggccgactc
     1081 ctctgctcgc gtcgagacag gactcaggga gcccaagagc aggagggtca tgaggagtcg
     1141 caaccctctg tcgaagcgaa tcatgggctc aggcggcggg taccgacacc cgctgaaaga
     1201 ggtcgcgaag gtcttttgtt ccaggatggt cccacgacaa cacacccata tcgactagca
     1261 cctgaagccg gggcgctggg tccagccgag gcaatgaggc ggcagggacc ggctgggact
     1321 tcaatacctg cgcgtagact ccgatccagg attccgtcac cgcccgcaat ccggggtcgg
     1381 tggctttcat tcttcccagc ctgacttcct gcagcaactt gatcaacggg tccgactgta
     1441 agagcgtccc gatcgcacga cgcacctgct cttcctgctc gtccatgatg aggatgatcc
     1501 agtgaaataa aaaataatta gttcatcgaa caggaaccgg ggccgcgagg atctccgcag
     1561 gagccacacc cgccgccgga actggaggtg acgggccatc cgccggttgc gccgactccg
     1621 aaggccgaaa actgcttgtc gaggcccgtg cccttgcacg cgggacaggc cggcacgacg
     1681 tcgccccgga ccaacagttc aaaccgatgg ttgcattcct tacagacgta ctcgaatatg
     1741 ggcatggtgc tgtactcctc gagatttaaa atattataag taaggtcggt tccacttcgc
     1801 aatcatcagc cgatgggagt ccacagatgt ggtcagaaga tacatcgttt gtctttcgaa
     1861 tcagtctcga agcgcaattc cccgacgact atgaaggccc tcacgatgaa catgcgtggc
     1921 ttcgtgagtg ggaactccag atcaaaccgg tcctcatcaa gaacctgttc gagacgctgc
     1981 gagaatatcc cgcgtggaag gcgcatgtcc gcaatcgcgg gaaatcgccg acggaagaaa
     2041 ttgaagtcgc gatgacccgg gatttttcac cggcacccta accggacgaa cagccatgcg
     2101 tcaggcgacc ggccgcggcc tctacattca cgtgcccttc tgtcaccagc gctgccattt
     2161 ctgcgccttc tatctcgaaa ttcatcacgc gcgtgccgcg gaggaatttc tggggtccct
     2221 gacgaccgaa ctgcgcctct atggtgagca gcggctcttt ggatcggaac cgttgcaatc
     2281 ggtctacttc ggcggcggga cgcccacgac cctgtccgct ccgcaactcg ccaccgtcct
     2341 gtcggcggcg agggagacgt tcggactcca ggatgaggct gaaatcaccg tcgaggccca
     2401 cccgggatcg gtcacgcggg acagcttgtc gcacctccgc agagccggat tcacgcgggt
     2461 gagcttcggc gccgaatcca tgaatcaact cgaattggat cgagtcggac gccccggcag
     2521 tcctatgagc accgtgcaga tggcgaccgc tgcgcggaaa gccggcttca ccgatctgaa
     2581 cctcgacctc atgtacggtt taccgggaca aaccatcgag agttgggagg cctcgctgcg
     2641 ccagatcgtc gctctcgccc cgacgcacct ctcctgttat gccctgacgg tcgaagaagg
     2701 caccgccttg caggcggcga tccggcgtgg gacggttccc gcccctgacg agacgctaca
     2761 aaatgagatg gaagatctgg cggaggacgt tctgagcagg gcaggcttca cgcgctacga
     2821 aatctccaac tactgccgtc ccggatttgc cagccgacac aatcagttgc actggaccgg
     2881 cggagactat ctcggcctgg gccccagcgc ccaatcctat gtcgcgggcc ggcggtttgg
     2941 aaatgtgagt gatctgagcg cctacaacgc ctctctccga accggcaccc tccccgtgac
     3001 agcatccgag caactcacga cagccgacga cgcctgcgaa cgactggtgt tcggactccg
     3061 gctgaccgag ggcatcactc tcggggaaac gggagccctg gcattctcag gtttgaccgg
     3121 caaagtaaac gaattgatcg gcgacgacat cctcgaacgc aggggtgagc gggtacgact
     3181 caccgcccga ggccggcgct atgcggatac tgtcgcagta gccctgctgg caagcctgga
     3241 gtcctaacta tcctcctccc attgacttcg ggacccgaca gaggggagac tgacgaactg
     3301 aaaggaggcc tccgtcatgc cagtgaacat ggaccccacc aagcggcgcc gacgtcagtc
     3361 cgccgaacag gctgcggccg cggccgacca ggacagcagc cagatcaagc gattcggaga
     3421 agataccgaa gccgatcggg aaaaggccat tgcagaagcg gaactgaaaa acctctggga
     3481 cgcgaccgaa gtcatcgata tggatacctg gtgattgagc gccgtaacca gcagagatcg
     3541 atacaaccag tcccgcgcac cactcgagca cacaccgatc cgatctcacc ttcgatcgac
     3601 agccatacga tccatgaacg ccttgtcccc ccgcaacgct ctctttcacc cgttccacct
     3661 ctgtgccccg cacacccttg aggctctgct cgcacggtac gatgccgtcc atttccgcga
     3721 ctacatggcg ctccgtctca ctccgctgat gggcaccacg gcctatcagg accgcatggg
     3781 ggacgatcat ccgatgctcg tcacgagcgg ccggctggtg caaggctatc cggtcagcgg
     3841 cccgctggac gacaccgccg tgacggccat tgatcgtgac ctgagcgatt cccgttggcg
     3901 caccctgttc catgacgggc tcagaaacga ccggcgtttt cagcgcgggc tcttcgacct
     3961 gacgcatgca atgaggatcg gatcgtcgct ggtgcccggc ccggccgcgt tgctccgact
     4021 gcttgagccg aaccgtgcag ccgcgctcta caacgtcgcc ctcgtgcaac gcctcgcaaa
     4081 accgaccctg acgctggacg aggcctatca atttgaatat ggtctggcct tgttgaagac
     4141 cgccgccgcg caggtctata ccattcgcct gtcccgagcg cacaacctgg tgccggtcac
     4201 cgactcccac acccaccatg tcctgctgag ccggaccctg gcccgtgagg ggatcgacct
     4261 cgcccacgaa tctatcggcg cgtccgtggg agcaccattg tcgcagcaca catcagggct
     4321 gcatgaataa accagagttg ggatactcaa ggagcttgat gccactgatc taccgctgtc
     4381 atgagttccg gttgcattca gccctatctc acctgctaca ctccgcgcct ggcgagacga
     4441 ccgcatgagc acactgaccc ctgccatgac ccctgaggcc ctcgacacga ccgacgtggg
     4501 caccggcgac gacctggaag cacgggtcat cgtgttcaac tgcgactgcc acacctatca
     4561 gcaggtcatc gcgctcttct gcacatgcat tcctggaatg aactcttccc gggccttcga
     4621 actggcgtgg cgcatcgacc acgagggcca ggcgacggtc tattcgggag agcgaaaagc
     4681 ggcggaggag atcgggaaga aactggcggc cggagggtta cgggtcggag tgcagtagag
     4741 aagggaacgg ggctggtctg cgccgaggat ctcgacgcag accgacttta cttggctggt
     4801 ttctttgccc ctgttgcctt ggtggttttg ggcttgctgg ctgctacagg ctgggatgtg
     4861 gaaggcttcg gactgctctt ggcgctgcca tgcgcaccct tggccatctt cgccggtttg
     4921 ctgctcgcct gactggcctt ttgttcctgc gcagccgccg ctttatagta ggatttatta
     4981 agtttgctga gcatgtcgcc gttcagaaca cgcacctggt acaactcgaa cagcttgctg
     5041 atggccttgg ggtcgccctt gcgcgccagt cccagaagcc gccgccgttg attcatttct
     5101 tcttcttcag tatgagacgc tgctcgccgc tccatagcgc tcctttcaaa ggatgcccga
     5161 aactcactgc ccaaatacgc caaattgccg gatgaaatgc cccgcagtat aacagattcg
     5221 gcctagccag tactacacaa acatcggtca tccgggagaa aatatcgcgc cctggcctgt
     5281 tcgatccgat ccggtccacc gatcgggcct cgggttgtag aggggggatt gaggtctcta
     5341 taatcagctt ttagccttca ccacgacaca aaggagcacc tgatggaaca tgctgcagcg
     5401 atcgatacac aaaccaccgt tgccgacgac ccccaagccc gcgccctgct ccaacgggcc
     5461 ttcgagagca cggccagatg gcaacctgat tttcgaggat tttcggccga cctgacggtg
     5521 aataccaacg gccagcaggt gcgcggaacc gtggtggtca agggaccgcg tgaggtcacg
     5581 gtgcagttgc cggacgaaac cattcagaag tgggcccagg aacagatcgg catgatcgcc
     5641 gtgcaccgcg cgccccgcaa gttcgaggaa tcggacggca aacaccgcct caccatggaa
     5701 gccggcgaag accacccgct cggtcgtcgg ttagacatcc acggcgacgg catgcagtct
     5761 ttctaccgaa tcaaagataa ccgcatcacg cagatcaacc ggaagatgcc ccatgtggcg
     5821 ttcaccatca acgtggaaga aagcagcacc acgcaggacc agaagcagct gaccaccaaa
     5881 tacacggtct actatttcgc accgcaggac gccaagctgc gcaacgtcga gagttttacc
     5941 gacacccatg tgcgggtcgg caactccgat ctgccggcca cacgccggat catttcgtac
     6001 gaaaacggcg cggtcctggt tcgaaccctg acgttcacca accacaagtt gctggcctga
     6061 tggatttgcg cacgacgttc ccgcgcagca tgcgcgttag attggaagga tatgtgcacc
     6121 tcgcgcgcat gatcgacaag tgccgcgctg tgctcgcggg aaccgaaggc gagtatatct
     6181 atccctgccc catggatgac cggctgatgg cattcgccgg tattaccgcg gcgcagttca
     6241 ccgcggccgt gaagcaacac ctgagtgacg aaggcgtcat ccaatggttt cgcgggatag
     6301 ccacgccaca cgcccaagcc gagctggaag agtggaaccg gatgatgttg agccgagggc
     6361 cgaccacgcc ggagaagcgg gcttcattta ataagtaccg ggacgccgtg gatccctccc
     6421 gcaccgacct caccagctgg gccgagctcc aggacctcga agaagggcgg acggtcccac
     6481 gaagagccta gggggcaatg gttgacatct gctccgcctg cgcgtcgaga gcagactcgt
     6541 cctacatttt cataggaggc acaacatggc cctgttacaa caggatcttg ttcctgcaag
     6601 cggtgactcg ctcattacgt tcgacaccga taccaacctc gaatggttga gcctcacgaa
     6661 gaccgtaaat ctttccattt cggacgtgcg gaacggcgcc gggggctacg ccaccaccta
     6721 cggttttcgt tacgccaacg gcgctgagct ccaggcgctg tggaaccatg ccggaatcac
     6781 aaggtttgcc cccacccagc ctgtcccagt gcccgatagc aactccgcgg gaatacaaaa
     6841 gctcatcgac tggatgggcg gcacgacgag ttatccgaca accggcacga tccagacgca
     6901 aggaatcttc atggttccac cagcacccgg ctacccgggg gtgggacaac tctggttctt
     6961 caccaacaac cctggtgggt cgtacgcaac cacggatatt tttcccaatg tacctcaagg
     7021 agtgacgccg gaagactacc ggagttctgc gctggcatcg tatctggtac gcaatcacgt
     7081 gtcccctgct cctccccgta accttcgggt gggcgaaaag taaggcgtgg ccatcgactc
     7141 gttttttcac ggcaactctg cgcatcaggg atgaagccca tgacgcggag aaggccgttt
     7201 catcgacagc aacctgccag ctccatgaag ccggacggaa tcggcttcat ggagcgtggg
     7261 cttcgaggca ggacagttca ccgcagcagt gaagagccgg cacgacgcag cgggaatccg
     7321 acggatgggt cgaatcgtat ccatacagat tccggctgta tctcccccga tacgtgttgt
     7381 tcacgattat ttgtacttac gacgcgcttt attgctgccc cgtgatcttc cgccagggct
     7441 acgcacccca ctcggaatgg tcacacaatc aggaaatgat ggcattgaga cgtactggca
     7501 tatgtatcgc atagaccctg acgatagacc tcgcgcaatc gcccactcca ccaccatccg
     7561 caggaggtac cgacatggct cttgttcaac agaatcttgt tcccgcaacc ggcgaccatc
     7621 tggtgacgct cgacaccgaa accaaccttg aatggttgag cctgaatgcg acggcgaatc
     7681 tgtcctacct ggaggtgctg ggcggcgccg gagggtacac caccacctat ggctttcgtt
     7741 atgccaccgg ccaggaaatc ggactgctct ggcaacatgc cggcatcaca aagtatggcg
     7801 tcacccatgt ggtgccgttc cctcaaagca atcacgtggc gatggaaacc ctcatcgaac
     7861 tcatgggcgg cgcaacgctg tatccatcgg tcaccagcgg ttccgtccta gtccagacac
     7921 agggcatgat gaagtttcga ggagcgggcg tcacgactcc catcaccccc atgagcgtcg
     7981 ggcaactctg gcttttcaag aacaatccag gcgggtccta tgcagacacc aacccggctg
     8041 gaaacgccgg aaaacgaact cctgaaatcg cctcctatct ggtgcgcgat cgaatcatgc
     8101 ccgccggctc catctctcgt ggaaaatccg ccaaggcggt gaaagcccgt agcgcgaaaa
     8161 agcagggctg atctcaagca gctccatgaa gccgaagatc atcggcttca tggagcacag
     8221 gcttccattt actccagccc tttggcgaac ccgtctgcgc ccccccacat tgatttgtcc
     8281 tccgtagagg gataggctac cacgtgtcct ggtcgcggga gatggtagct cctcacgggc
     8341 atactcgctc cgccaccggc gaccatcacc gtatcaccgc aagaggggtc gtatggaaga
     8401 cctcagtccg cctgatcacc atcatcgccc agacaactaa cctgccccct cgggtacaca
     8461 gtgaggcagt ctactcctgc acacgcatgg tgatactatg acgaccaaga caacgggtga
     8521 ccaacccctt ttcacaccgg aacagacgtt cagcagccac acctctgcca agaacattct
     8581 tatcgccgtc gatgactcgg acgagtccgc ccgcgccctg cactatgtcg gcgtgctgct
     8641 ccgggacatt caggacgtga atgtaacatt gtttcatgtg ctgaacccca tgcctcggga
     8701 actgatggag cacggcggct cggagaaccc tgagacggaa caccatctcg gcgaacaact
     8761 ccgcaaggac caggaagaat ggatacgcac tgaaggcgcc atcgaatatc ccatcctggt
     8821 gacggcgatg gaacgactcg ggcagaccgg atttcctctc gaccatgtca cgctcaagtt
     8881 gggacatgaa cgcgacatcg ccgacagcat catggatgaa gccagggccg gaggatatgg
     8941 caccgtcgtg gtgacccgac atgggccgac gggaaccaag cgactcttca gcagccgcat
     9001 caccgaccgc ctggtgcggg acctctccgg agtcgcgctc tgggtcctgg gataatcttg
     9061 tgacaggcgc aactatcgtc ctgaagcatg agcgttaggc gcgcctcgga ggctaccatg
     9121 aactgcacca gttgtgagac tgcgctcccg ttgcgggccc gcttttgtcc cgcctgcggg
     9181 gtgcgcgtga cattgccgga ggatccggcg ttcgatgagg cagccttgca agcggccggg
     9241 tgcggggacg agttgaacca acgaacggct ccggcgcccc tcgaccatga agccttgaag
     9301 gcacggctga tcgattgtta tgtgaatttt tccatggccc gggaactatc cggatggcta
     9361 caagcactcg ggcgtccatc aacgggaacg atgcaggaga aactcactcg tttgcgacaa
     9421 caagcggatt cgctggtact gccggccgaa agtcttcctc gccagaccat ctggtacctg
     9481 aaccgatatg acgagaagat tctcacggag atttgcgaag agcttggtat cgacaacagc
     9541 ggacccaaag agcggcagat caccaggatc tatcatgccg tgggtctgcg tgagggctgg
     9601 ctccaaccgt tgtcggaaga tgctcgaaat atcatcaccg agacgtttct gcctatcctg
     9661 agagcggtcg atcacagaaa ttatgctgac ctcgaccagt ggggtgaact cggagacctg
     9721 ctcgacaggg aaagtaggac tcggctcgat catcaaggcc atgggagcgc cttcatggcc
     9781 gtcctcattc ccagcttact ccaagaggcg catcgggatc tgcttcaaca tgaactcgat
     9841 gccagagccg ggaaaccctt ctgacgacac aacaccggtc ccctcgggtc tctgacagca
     9901 gaggcccgaa agccctcctc tctgccgaac cgaccgtcgc agtcttcatg aactgatgta
     9961 ttggtggaga taggcgatga gcgcctcctt ttcctccatc aacagcgcaa ccatatcccg
    10021 gagcgagaca atcccgataa actcctcgtt gtcgaacacc aagaggtggc ggcagcgatg
    10081 ttccttcatc agattcaaac aatgattcgc cgtatccttc ggactcacgg tgaccatatt
    10141 tttcgtcatc acatccttga tctttacgtt ctccggatct cgcttccgac cgacgacgcg
    10201 catcatcaag tctcgctccg tgaaaacgcc ggtgatcttg gaggaactgg tgatgagcgc
    10261 cgatccgacg aattcctcgg ccatcagcgt cgcggcatcg agcacggtat gattctcgtc
    10321 gatcgtcacc accctgtgaa caattttcga ggcaatattc cacgccatcg ccgcctcctt
    10381 tctattggat gcaggtggac ggagcctgtg ctttatgcca agtacagggg ccgggtaacg
    10441 ccgacgccca tcgaaaccgg agtcagagcc ccctttatcg aatgaccagg atgggacagg
    10501 tcactttatg caagaccgca tgtgacacgc tgccgagcag gaagtgttca aggcctctcc
    10561 aaccatgcga gccgacaatc acaagatcgg catggcctgc cgctttggta atgcaatgag
    10621 ccggatcacc atgctcgact tgcgcggtga cagcaggata catggtccgc aaggctgcag
    10681 ccgtttcgtc cacaagttgc cgggcatact gatccatttc agccgtccat ttgtcgtatc
    10741 gaaattccat cgtcgaatcg ccgatgggaa tggtcggcac gacggtcaag agggacagtt
    10801 ctatcggccg gctgaaccgg tgagtgctca accagctgcg cagcctgact gaatcctcat
    10861 acccttccac ggtgagcaac acccgtcgaa tgctccccgg gtcgtccctg accagcaggg
    10921 ccgagcaggg agcatgcagc actactcgat gcgagacgct gcctaagacc aattcagcaa
    10981 ggcgtccacg cccccgtgat ccgaccgcaa ccaagtcggc atgagccgaa cgaatcgcct
    11041 ccagcacaac cggcgccggg ttcccgatgt tatagactcg ctggatggaa ggaatcgtga
    11101 ccggcaccaa tgcgccggtt tgatcaagca ggcgctctcc tgctgcaacc atcccctcgc
    11161 gcagtgctcc agcacttttg ctgccgagcg gttgcgcaaa gagcggattt tcgaacggcc
    11221 ggagatccac cgcgtggatg agagtcaatg attcatgcgt gaagagacgg cagacgacct
    11281 taacggcgtt gaaagactgc tcggaccagt cgacggcaac gacggtgcgc atcgcagcct
    11341 cctgacgcga aggcgtgtcg gcaatcgctc ttctaggagg cagagaaccc tcattcagta
    11401 gagcgacatc cgcagattgc aggatgcgcc atcatactcc ctatccggcg atggaggtca
    11461 agcgcagcga aaatatttgg ctggtagggc tcgggacgga gctggccaat ggagagaaac
    11521 ccatcgaata tcgcagcgtg caatagaata agcacgcccg agtcgtgaat gggaccagga
    11581 gtatgccgcc gggaccggat cagctccctg tgtgcaaaga ccgctagcca gcgtaacgca
    11641 catcacggac atcggcgagg gacagatacg cctctcccca ttgatgagag ctctggagac
    11701 tccaaggctc atcgaattgg atgggcctga accgtagacc atagagccct ccatacacga
    11761 ggtacgccaa ccccgatccc gaatcttcaa tcagctggac gtaggcggga catgtgggag
    11821 cctgttcatt gtcgctccag cccgtcaccc agcaccactc accggtaggg tttcgctcat
    11881 acagccggac ttgaaccacc ggtcgagccg gcccgacttc catgaagcgc gcgaagaagc
    11941 tggtgtcgca attgtcgttc gattcaactt ccacgatcat gaggtcctca tgcaactgct
    12001 ggtaaattca tcgcggagga tggccgactg aaacattgcc cttaccgagg ggccgcacaa
    12061 ttcaatcata ggttgatctg ttgccaccag cataggcatc atcttcaggg ctacgcctga
    12121 ggatgctcct caggctaagt cgtcgctgtg ccggcgaaga aattttcagg aataagaaat
    12181 ccacaggatg ctacgcctcc gtaattcatc tgaaggccag agtttccagc atgtttattc
    12241 acacaacatt ccatccgatg caaaggagaa tcaagcatga cctctgagaa cgttccggca
    12301 gttgtggccc ggtattttag cgccatagga gagatggacc cggacgcctg ggccgcctgc
    12361 ttcgcagagc agggaaccag ttacgagcca ggctccccca cacccctcca aggccatgcg
    12421 gcactgcgac aatttctagc gggggttctc ggggcctttg aaaagatcac tatgacccag
    12481 gaccatgtct tcctctccgg caaccgggtc gcggtgaagt tcacagggcg tggcaccggc
    12541 aagaacggca ggcaagtcac cttcgaaggg atcgatgtat tcgaaatcaa ccaggaaggg
    12601 aaagtccaaa ccatgtgggg ttactggaac ccggccgcat tgatggcgca gctgcaggga
    12661 tgaagcagta tttccatctc ccgcatagcc aatttctggt atgttcttta gaaatgtgac
    12721 cgtcctagag cccgatattg tcgaagcgtt gtgggactcg gcgtcggtca actcagccct
    12781 ccgttcgctc ctagagatat ggcaaacaac gagacgcctc accacccggc caaagcgagc
    12841 atcacgcaaa aagccccctg tgtaaacggc ctgagacaca gtcgatcccc gcaaaccaaa
    12901 ctcatctgct tcgggatctg tcgtatctcc aagaaagtaa gtaagtgcag aaacgtcaat
    12961 agccgccact tctagctggc gtgcacgcac tctaaatcat tcccgacacc atttcttcat
    13021 cccaactcat gccacccctg gcgaccaatc gtccgactga caggcgaaat ttaaggggtc
    13081 cgaccccttt tatctctcgc cacacgagaa cactccgagt tctcgcaaga attgctccca
    13141 tgcctcaatg gctttcagct gaatggtgta aagatctccg atcgaagtac catcggaatt
    13201 cagaacgtct gccgtgacaa ttagattggc agagcctgtc ccggccactc gttgctctcc
    13261 ggaaggagcg attgagaaag caacacttcc ctcgggccct gctcttgtcg taagacgcat
    13321 gacgcggcga aggttggtca acctaggcgc tttgccagaa aacccaccgt ttcgaggcgg
    13381 ataaccatgc ttgtcgttat tggataggtc ttggatgaca gcaaccgatt cattcgcctt
    13441 aagaaactcg tccacctgtt gcttgtttac ccccttggca gacttcacta tccatgaacg
    13501 cgtgtgatct cgaagattgg caatgttgtg aatgactgaa tgcgcgaggt tctccaactc
    13561 tgcatcagac agactgccgc gaaaatcttg aaatacgcct cgaccttgtg tgcttgagat
    13621 aagaacagga agagctttgt taatatcatt gtcaattacc gcatccagag cggcatagat
    13681 acgctgcaat cggacgctag cttcgcctgc agtcatactg cctgaaagtg ataatcactg
    13741 actagcctat tgcgctgatt acttcgtaaa atcctgagca caacggaagc caatggagaa
    13801 gttagtaagc gacgggtcag accaactcgg tatccggatg gggaggcccg tcgcaggagt
    13861 gaaccacgat cctccacgca gtactttgaa ctggcctagc accggccctg ctggattgcg
    13921 acttatatcg gttttgtagt actggccttc aatatcgatc cagtcagcta cccattcttc
    13981 agcatttcct gccaagtcat aaattccata gggactgatg tccaacgcat agcttccaac
    14041 cttggcgaga gtgctataac ctttccacca atcgtctcgc ttatcgaagt ttgcatgctt
    14101 ttgagttggc acttcctgcc cccatggata ttctcggcca tcagtccctc gagcggcttt
    14161 ttcccactcc gcatccgttg gcagacgttt atctgcccaa ctgcaatact cgtgggcgtc
    14221 ataccaagtt actccgatca ccggccggtc agcatcaatg ccaagatttg attcgctcca
    14281 cctattcgga tgcctccgat cagaattcgc taggaatttc gagtaccgag agacggtgac
    14341 ttcaaactta tccaggtaaa acgcatccag ataaatccga cttggcggcc gcggaatctg
    14401 atcgctaccg ccccaaatga attcaccggc cggaaccagt accattgcgc ccccgtcctt
    14461 tccgttgatt tcaatggttg gttcatgagc cggaaaattg aactgcgctg aaactgtcag
    14521 ttcgggattg aacccctctg atcttaagta ctcttgatga ctggcaagca aaaacagcag
    14581 tgtgaaaagt gttgcgacaa gaactttctg aacaaacata tagggtcctt tagccctaac
    14641 agcacggctc tcaattagct cgttcccggt attccaatgt gcgccacgcc cgtcgggtta
    14701 ggtccttcgg atggcgcggg atccgggagc acatccctta atgctccttc aaaacttgtt
    14761 cgttttccat ctccgggagg ggccgtcgag ctggttccaa ctgcgcccgt cgagcgaggt
    14821 ccctactgag ggcgcgcgct ccgagagcac aattcgatgc tccttccaag cttgctcgcc
    14881 ttgcttcacc agcgggtggc ccgcttggtc tcctactgcg cgcgtccaac gagggccttc
    14941 ttaggccgcg cgttgcgcga gcacaggaga ctaacgggcc accctccctc tcacttccac
    15001 ccctccccgc tcatcccgcc gccgtggccg ccgaagttcc cgccgaaccc cccttggccg
    15061 ctcccccaat attcgccgcg ccgcaggcgc ccatagggat ggcggagatc cgggcggctg
    15121 agccaccaga gaaaccagag agctcccccg acggtaaaga tcgtgatcca gaacccgagt
    15181 ccgcgaaagt gcgtcttctt aatggagccg acgcgaatct cctgcgagac cgacgccaac
    15241 cccacgacgg ttcgatagag tccttcgccg aagtggccgt tcttgacggc ggggtcgaga
    15301 tattcatgcc cgaccttccc gaccacattc ccccccatca ccggcaacat ccgtcgtccc
    15361 atggtgacgg ccgcctgccg ctcctgcacc gacagaagca ccaatacccc atgctcattc
    15421 tgggccgaac cgatgcccca tttctgatag agcccgaccg cgtagtcatt ggccgaacca
    15481 tagggtttca gcgtgggcac ggtgacgacc accatttcca ccccggtctt gcgttcaaga
    15541 tcctggcaga ccgaacggat tcgcgcgcgc cactcggcat cgatcacccc tgcatggtcg
    15601 ctcacatacc cctgcgggtc ataggaaggc accggctcgc gggcgaaccc gaccggcact
    15661 gcgaccaaca gcagcagaag gcccaacccc acaatggaac agatggtgat actgaatcga
    15721 cgattcatcg cggcgtcacc gtcccgtcca tgcggatcag cgcgatcaat gcgtcgagat
    15781 ttgcagcata ccgttcaaac aaccgtggaa cttccaccgg accgggagaa ataatgcctc
    15841 gcttcaactc aagcacatcg ttcaatccgg cgagatcgag tcccgtcaac gtgcgaaccc
    15901 cggcgagcaa cgcatccgac tgaaacaagg ccggctgtcc ggcaatccgt tgcaaaccgc
    15961 gcaagcaggg cagcaaggcg gtcaatgaga gcggcaacaa catggtcatc gcttcttccg
    16021 tgccgccgcc ttcgatatat cgctgccgca gacgcaccag gttcccttga atgccctgct
    16081 gcacttgata cgccaaatgc gtccggtcga tgtgcatgcc gatgaacgga tcccgccccc
    16141 agagcacgcg atgttgctcc tgaatctcca ggtattcgag ggggaagaca gtgccgggcc
    16201 gcgtgagttc agcctccgtc accaggagcg gcaccacgaa ctgctcacgg ctccaacgct
    16261 tatgcgcctt ggcataccgc ttcaggccct cgcgctcgta cccagtcagc atgagcaaca
    16321 gattcagatt cgaccggccg ggcaggaatt cgcctcccac ggcgctgccg tagagcagga
    16381 tgccttccaa ctgcgaaccg agcaacgtcg tggtttcctt cacgtatcgc ttgagcagtt
    16441 cttgaacctc tgagggaata tcgagtgcag tcggttgagt cacaaacacc tctgtcgtcg
    16501 tgtaggcagg atgctgaaaa atcccgccag cggcgttctc gcttcgtgca gaccctcaac
    16561 gtaccgcaag ggtacgcctc ggccctgcac tcgctgcggc cttgctggac ggcctttttt
    16621 gagcatcctg tgttgggttt attgttgccc gaactgaaac atctccctgt cacgcctcgt
    16681 ccgatgatcg cacctgggcg cgcggatctg gcaagagtcc ggcggccatt acgcggtcca
    16741 acaatgaaaa cggcggtcgg cttcgcaatc cggccgtggt cgagagcaca cggtagcgca
    16801 gcctggcatt ccggtcgagc gtccggaaca cccgatctcg caaataggcc accaccggat
    16861 taccggtatt ccagaatcga cattgctcat cggccaaccg ctgcaacatg gacacctgtg
    16921 gccgccgggc cacctcgaat gcgcgcaacg attcagcaga gaaatcgttc ttcttcaacc
    16981 aaccgggaat aaggtccgcc acgacgaccg cgtccaccat cgcctgcatc cgcccctggg
    17041 atgcatgcgg attcatggcg tgggccgcat ccccgatgag caaggcgccg tccgccaccc
    17101 atcgatccgt tttcacacgt ccggtgggca tgtaacccgt ctggctccaa tcgaccagtt
    17161 cttggaccat cccttccatg ccgggatcga tccggaccca ggccctccgc aacgcctcca
    17221 acccctgagc tttgaccgcc tcatacgatc cggccttgat catatagaag gcatagacct
    17281 gttgcccggc ggcaggaaag aggccaagga tttcacgctt gcccaccaga taccgtgcct
    17341 cgtcgaagcc cggcggagcc ttgagaatgg caatgagata actttgggga taccgatgga
    17401 gttgggccgt gataccgagc gactcgcgta ctttggacaa cgcaccgtcg gcgccgacga
    17461 ccaaccgcgc agagatatcg accggttccc cgtgacgtgt tgcctgcaag ccgatcaccc
    17521 gcgagccgtc gaaccggagg ccggtaaagg tcgcgtcgta ccagagcgcg ccgggattct
    17581 gttgttccaa cgcatcgagg atggcatggt gcgccacatt cggcagcgtc accagggctc
    17641 gattgtaagg agccggcaac tcgcaataat ccaccgtaca gagccgttca ccgcccgccc
    17701 ggcaaaagtt gaatcgacgg accgatcgca ccgcgtgggc aggaagcttg tccaataatc
    17761 cgagacgatc gagcacgcgc tgaccgttcg gttgcaggat ctcgccccgc aatccctgcg
    17821 gcggcccggc agcccgctcc aacaccagcg accgcacgcc ctgctgcgcc aacatcaacg
    17881 ccaacacggc accgccgccg cccgcgccga caatcgccac atctgtctcg atcattgcac
    17941 ggcccatgcg cggctcgtcc tattgggtcc aggtcataaa tcgttcgtgg tcgttccaca
    18001 tggtcagaaa cttctcgcgg gccttcgccg tcaatcgcgc atcggtgatg acatcgaggc
    18061 gttcgtcatt gtaccggttc ccgctcgtcg tgtggttggc tgaaccggag gtattgatgg
    18121 catcgtcgat cacgacctgc ttcaggtgca tcaatgcatc atgccggttg atcctgatgg
    18181 gcacgcccgc cagtcgtaat gtattcacgg cgctatgctg cttcggatcg ttgagacgtt
    18241 cacgatcggt aatcacccgg acatccacgc cgcggcgttt cgcctccacc agcgccttca
    18301 ccaccgaagg cgccgtcaac gcatagacgg acacatagat gtagcgcgtc gcatgcgcat
    18361 agagaccgac cacccgatcg atgggctgat cctccggcgc atagtagaca tcaatcgtgg
    18421 cggaggcata gacaggaacg tgcgtcaggc aaattaaagc aacgacagtc cagacgacca
    18481 gctgagagga gatcagacga ctcatgcgga gactagctgg cagcagaggt ttgatgagcg
    18541 catgtgaatt tcgatgagtt acacatgaaa tacggatgga ttcccgcagg atgctcaaaa
    18601 tgttcgtcca gcaaggccgc agcgaatgaa gatccgaggc gtacccttgc ggtacgttga
    18661 ggatctgaat gacgcgagaa cgccgctggc gtacattttc agcatcctgt tgaagcggat
    18721 cactcgaggt cgaacaaagt tcgaaaatgg tcgtccgatt cctcccaggc ttgcggagac
    18781 gctttggaca accactcgcg caaaaagccc ttctgctccg gcgtcaacat ctgcttgatc
    18841 tgatgcgacc ggccctgcaa gggttggaga aaaccggcgc gcgtacgaaa atcgagcgtc
    18901 gcggtccgga catagatgtt cgtgtacatg gaggggctgt cgtcctcgcc ttccccctgc
    18961 acccacaccg acaaaaattt ttccatctgc aacccggcgc tgtccaaggc cggttccgca
    19021 tcatgaaaca gctcgttttc cgactctttc agcggagccg cctcatgcgc gcgaaataag
    19081 aaatttttct tccacatgcc ctaccctcca gaccggcgca tactgtaagc taggcatggt
    19141 gaaagtcaag aaagcggccg cctgcctcgt gcgactccgc agcagcctcc gccacatcga
    19201 cccatcgggg tttgttgcct tgacaaaaca agaatgcgtt tgctaacttc ttgaaattat
    19261 ttagcagttt cagacaaaca cgtcgaatca aaaactgtcg agggtctgcg cttactgcgc
    19321 aacaagaatc ccttttgaca ggatgcgcaa aatggccgtc cagcaaggcc gtagcgaacg
    19381 aagggacgag gcgtaccctt gcggtacgtt gaggacctga gagatgcgag aacgccgcgg
    19441 cggacatttt cagtatcctg tcagactctt accttctcag gagcagatat gcaggtcatg
    19501 atcaacggca agtccgaaga gatcgcaggg ggcagcgtgc tggatctctt gaaagccaag
    19561 agcatcgaac cccaaatggt cgcggttgaa gtgaatgaca ccatgctcga acggacacac
    19621 ctcgagacca cacagctgaa agacggcgat catgtggaat ttctgttcta catgggcggt
    19681 ggccggtgac cgtcaaggcc catgcagata tcaccgagtt gatcggcgga accccgctgg
    19741 ttcgcctcaa ccgcctgagc aagccaggcg gcgccacgat ctacgccaag gtggaatcgt
    19801 tcaatccggg cggcagtatc aaggaccgca tctgcctgaa catgatcaac gaggccgagc
    19861 gcctgggtaa actgaagccg ggcggcacca tcgtcgaacc gaccagcggc aataccggca
    19921 tcggcctggc actggtggcg gcagtgcggg gatataaact cattttggtc atgcccgaaa
    19981 gcatgagtat ggaacgcgcc agcctcctgt cgtcttatgg agcacagctt gtgctcacag
    20041 cggcgtggga aggtatgaag ggctcgatta aggaagcgga gagcatcgtc gcgcagaatc
    20101 cgtcgtatta catgcccgac cagttctcca acccggctaa tccggatatg caccggaaaa
    20161 cgaccggccc ggaaattgtg aatgcgctgg acggacgcgt ggacgccttc gtggccgcgg
    20221 tcggcaccgg cggcaccatc acaggctgcg gcgaggtgat cagagaacgg aatccggcag
    20281 ccaagatcat cgccgttgaa ccggccggtt cgcccgtgtt gtcgggcgga gacccaggtc
    20341 cccacaagat ccagggcatc ggcgccgggt tcgtcccgaa ggtgttgaac cggacgctgc
    20401 tcgaccgcgt ggtgaccgtg accgacgatg aagcctatca aacggcgaaa ctgctcgcga
    20461 agaaagaagg actgctggtc ggcatctctg ccggcgccaa cgtctttgcc gcgcaaaaga
    20521 ttgctgaaga gcttggaccg ggaaaaaacg tcgtcaccat tctctgtgac accggcgagc
    20581 gatatatcag catcgaaaag tatttcaaca tttgaccatt tacaacggat gattgagggg
    20641 gatgctcaaa atggtctctc agcaaggccg caggtgagag gaaaccggag gcgtaccctc
    20701 tgggtacgtt gaggatttcc tcgaatcgag aacgaagctg aaggccattt gcagcatccg
    20761 aaagattttc aacatctgag cttgggacag accacttagg gagaggaggc tcaaaagcgt
    20821 catccagcaa ggccgcagcg agtgaaggtc cgaggcgtac ccttgcggta cgttgagggc
    20881 gtgaacgatg cgagaacgcc gctggtgacg gttttcagcc tccgtcggat atggaattta
    20941 cagaaacaca aatcaaccgc tacagccggc acattcttct tcccgaagtc ggaggcaaag
    21001 gccagaagaa gatcgtgcaa gcgaaggtcc tcatcgtcgg cgccggaggg ctcggctccc
    21061 ctgccgcgct ttatctcgcg gccgccggaa tcggcacgat cggcctcatc gacagcgatg
    21121 tcgtggatct gagcaacctg caacggcagg ttattcacca gacgccggac gtgggccgcc
    21181 cgaaggtcgt ctccggcaag gaaaagattc aggcgctgaa tcccgacgtg aacgtggtga
    21241 tgtacgaaga gcgactcacc gccggcaatg cgctgaaaat catgagcggc tacgatgtgg
    21301 tcatcgacgg cgtcgataac tttccgacga aattcctcat caacgatgcc tgcttcttcg
    21361 ccggcaaacc gctggtgcat ggcgggattc ttcgattcga tggtcgcgtc accaccatca
    21421 tcccgaagaa gtcggcttgt tatcgttgcg tctttaagaa gccgccgccc gaggggctcg
    21481 ttgcctcctg ccaggaagcc ggtgtcatcg gtgtgctggc cggcatcatc gggaccatcc
    21541 aagccaccga ggcgctgaag ctgatcctcg gcatcggtcg cccgttgacc gatcgcttgc
    21601 tggacttcga tgcacgacgc actcagttca gagaaatccg catcaagcgg aaccccgatt
    21661 gtccgctgtg cggcgaacgg cccacgatca cggaattgat cgaagacggc gacgtcgccg
    21721 ggccgacctg tgcgctcccg ggtcaacgta acttgtagcg aaggtgtctc catgtcgaaa
    21781 atgaaagcgc tcgtgtgccg ggaatgtggg aaggaatatc cacccaaagc catccacgtc
    21841 tgtgagatgt gcttcggccc gctcgaagtc aaatacaact acgacgagat caaaagcacg
    21901 atctcccgga aaaagatcga acagggcccg aacagcatgt ggcgctatat cgacctgctg
    21961 ccggtcgaaa gcaccgcgat catcgggccc catgcgggac tgacgccgtt ggtgcgggcg
    22021 aaaaatctcg gcgcctatct gggcatcgac gagctctata tcaagaacga tacggtgaac
    22081 catccgaccc tgtcgttcaa agatcgggtc gtctcggtcg ccctcacacg tgcgcgggaa
    22141 ctcggctttg aaacggtggc ctgcgcctcg accggcaacc tggccaactc cgtcgcggct
    22201 cacgcggcgg cggcaggcat gaagtgctac gtgttcatcc cggccgacct tgaagcggcg
    22261 aaggtgctcg gcaacctgat ttacaagccg aacgtggtcg aggtcgaggg caactacgac
    22321 gatgtgaacc ggctctgcag cgaaatcgca ggtgaacatg gctgggcctt cgtgaatatc
    22381 aacattcgtc cctactatgc cgagggctcg aagacccttg cgtttgaaac ggtggaacaa
    22441 ctcggctggc gcacgccgga ccaggccgtc attcccatgg cctctggttc cttattgacg
    22501 aagatctgga agggcctgca cgaaatgcat gcgctggggc tggtcgattc cgtccgcacc
    22561 aagatcaacg gtgcgcaggc tgaaggctgc tccccgatcg cgaccgcctt caaggcaggc
    22621 cgggactttt tcaagccggt caagccgaag accatcgcca agtccctcgc catcggcaac
    22681 ccggccgacg gctactacgc cttaaaagcc accgcagaaa gcaaaggcgc gatggacgcg
    22741 gtgacggacg aggaagtcgt ggaaggcatc aagctgctgg cccagaccga aggcattttc
    22801 gccgagaccg ccggcggcgt gaccatcggg gtcttgtgca agctcgtgaa gcaggggctg
    22861 atcaagaaga gcgacgtcac ggtcgcctat atcaccggca acgggctcaa gacccaggaa
    22921 gcggttgtgg acgccgtcgg ccgtccgttc cgcatccagc ccagcctggt gaacttccaa
    22981 aaaacattca aaatgggaaa gaacagtggt ggtgactcat gattaaggtt cgtattccaa
    23041 cgcccctgcg tcccctcaca aagggtcagg gcgaagttga atccgcggca gccaacattg
    23101 tggacatgat cggatcgctc gacgcgacct atccggggct gaagaaccgg ctctgtgacg
    23161 aaaagggcga cttgcgccgg ttcgtcaaca tctacgtcaa cgaagaggac atccgtttcc
    23221 tgaacgggaa agaaacctct ttgaaggacg gtgatgaagt gtcgatcgtt cctgcgattg
    23281 ccggaggata gccatgacca gcctacgctt tcatattcgt tttcccgaga acaagatcaa
    23341 ggagccgatc atttatcaga tcggccatga gttcaaggtc gtgaccaacg tgcgtcgggc
    23401 cgatgtgcgg gagacgaccg ggtggatgga tctcgaactg accggcgaga ccgaagaaat
    23461 cgaacgcgcc atcgacggca tccgcaccaa aggctgcgtc gtcgatccca ttgaattgaa
    23521 cgtagtggaa taacaatggg tgaagcgcac ctcgtgaagt gtatctcgtt ttagggccag
    23581 accgcttcac gtttcacgaa cgacgagaga cgagtcgaat ggaatttacc gaacagcaaa
    23641 tacagcgcta tagccggcac atcatcctca acgaggtcgg cggcaaggga caggtcaaac
    23701 tgtccaaggc caaggtcctc ctgatcggag ccgggggact cggctcgccc gcagccctgt
    23761 atctggcggc tgccggcatc ggcaccatcg gcctggtgga cggcgacgtg gtggatctct
    23821 cgaacctcca gcggcagatt ctccatacga ctgccaccat cggcgtgccc aaggtggaat
    23881 ccgggagaag gatgctgtcg gccatcaacc ccgacatcac gatcaagacc tatcagctaa
    23941 acgtcgatac cgagaatatt cttggactcg tgtccgacta tgacatcgtg ctggacggct
    24001 ccgacaattt caccacgcgc ttcctcgtga acgatgcctg tttcttcgcc agaaagacac
    24061 tcatctcggc cagcatgttt cgcttcgagg gacagctcac aaccatcaag ccgcatgccg
    24121 gctacccttg ctaccggtgt ctctacccgg aaccgccgcc ggccggattg gtcccgaact
    24181 gtcaggaagc cggtgtattg ggggtcctgg ccggaaccat gggcatcctg caagcctctg
    24241 aagccatcaa ggaaattctg ggcatcggcg agacaatcgc ggacaagctc gtgatctacg
    24301 atgcgctgga gatgaagttc cggaaagtgt cgcgcccgaa agacccgcgt tgccctctct
    24361 gcagcgccac gccgacgatc aaagacttgg gcggggacta cagcgtcgcc tgcaccatct
    24421 gatatgcccg atctatcgat tccgcaacac atcctggatc agatcgtcgc gcatgcgcgc
    24481 gagttggcgc cgttcgaatg ctgcggcctg ctcgccggca cgaacaagac cgtcacacac
    24541 ctctaccgca tcaccaacat cgtggccgtc gaaggcgcgg aaaaactctc cacctttgat
    24601 gacgacaagg tcgcccatct tgaacggctg tccccggaag agcgagctga gatcgccttc
    24661 gtgatggaca tgggggattt ttcggccgct aagaaagaca tccgcaagaa tggcttggac
    24721 cttcaagtgg tctaccattc ccatccgaaa gacccggcgc gcccctcgca caccgacatc
    24781 aagatcgcca ccgattacga agaaatctgg gaacgcatca atctccccgt ccccgcctat
    24841 ctcctcgtgt cactcatgca ctcccccgca gcggatattc gcacctactg gatcgccgac
    24901 ggcaaggttc gccctgccga cgtgcatatc atgtagtcat cgcgccgcta gaacaaaacc
    24961 atctcgctcc ctctaccaat cgaccggcaa cgatgccata attgtgcttt gaccttgtca
    25021 tcgaagggag cattcgatgg gatttggcct gctcattgtc gcactcattc tgtgcctgac
    25081 cggggtgacg aaccctgaac acacctccgc cgctgaaaag agctactaca gcccgatcat
    25141 caacgtagac gtcgataaca gccgcattct gatctcaacg ctcggcgcgg tgttttgggt
    25201 ggaggtcccg gaggaggcca aagctcacat cgagaagcta ccgcaatcag ggctcgtaga
    25261 tatcgtggtg gaaacacgcg aagggcaacc gccgctgctt aaaacttgga aggtcaagtc
    25321 cggcgaatcg acctgcctgc atttcgacgg aaaagtctgc aagtaaagtt gagccgtcgt
    25381 cgctgttgcc tgtcggtggc ccaagttccg acaggcacac atgtagagac agtcagttat
    25441 gacccggaag tcatgcggta gcgctacgct actccgaact ccccaacatg tcgtcgacta
    25501 acggtcgatt aatatccgta caccccatac aaatggtatc gaacaacgat tcgtgatcgg
    25561 acacgaagtt gcgctgatcg accagattct cagcaaagac ctgttcataa cacacgtcac
    25621 acagcatcat gaggccgcgt gacacggaca ctgttttccg gcctgtactc cccgccataa
    25681 cacactccct ttcgtatata tgctccggtg cgcgcagcct gcgctacacc gatccctttt
    25741 gcctcgcgat ccagaaatct tcaggctcta gatcaacacc acacaccgcg cactcataaa
    25801 tcgactctcc ctccggcaac cggatttccg tccggtcgat cctgccccga cagacatgat
    25861 aaaaatgccc ccgggcgtcc tcattgtcga gcggatcact ctcgtaaatc agcaccatat
    25921 cccttactcc ctttgatcat cattcatgaa gtctgcgcaa ccgtaacact caatattcaa
    25981 tcgactcttg gatattgacc acgtatttct tatacatatt cgtaaggatg ctcaaaatgg
    26041 ccgtccagca aggccgcagc gacgtgaatg agcgaggcgt acccttgtgg tacgttgagc
    26101 tctttcacga tgcgagaacg atgctggcgg actttttcag catcctgtta aattttaaac
    26161 tgggcctcat acagccccgc atacaccccg ccccgtttca tgagggcctc gtggtcgcct
    26221 tcctcgacaa tcgtcccgcg ctctaccacg atgatgcgat ccacgtcatg gagcgtggcc
    26281 aggcggtgag caatgatgaa ggtcgttcgc cccttggtca gatcgttcag ggcttcacgg
    26341 attttcactt cggtttcagt gtcgatattg gaggtggcct cgtcgaagat cacgatgggc
    26401 ggatctttca gcagcacgcg cgcgatcgag acccgctgct tctggccaac cgacaatttc
    26461 accccgcgct ccccgatcca gctatcatac ccgtccggca gtcgggtgat aaagtcatgg
    26521 gcacgcgccg cacgggcggc cgcttccaca cgctcatggc cggccgagag gtcgctatag
    26581 acgatattct ggcgcacggt gccgttgaag agaaagggct cctgctgcac caggccgatc
    26641 tgccctctca aaaagtctag cggcagctct cgcacatcat gcccatcgac cagcaccgcc
    26701 ccgctacgta cgtcgtaaaa ccgcatcaac agcttcaaga gcgatgtttt gcccgctccg
    26761 ctcggcccga ccagggcgat ccgttcgccc ggcctgaccg tgagattcac atcggacaag
    26821 acggtgccgt ccttccggta gccgaacccc acatgccgat actccaccgc gcctctcagc
    26881 cgctcgacgg gccgcaccgc gccgggccgg tcttcaacct ccggtttggt atccaggatc
    26941 tcgaacacgc gctcactggc cgccaacgca tgctgcagca tgtgattcaa cgagtggatt
    27001 tcgttgatcg gcgtataaaa gagcccgagg taggagagga acatcaccaa ctgaccgacc
    27061 gtcagttctc cggccagcac ttcccccgcg ccgtaccaca ggatcaacac cgtgccggtg
    27121 ctgcccacaa acaccatacc cggccagtac caggaccaca ggaacatggc ccgcaggctg
    27181 ttctgactgt atgtctggct caatgtctcg aaccgttcgc gttcatactc gtgctgattg
    27241 aagccgatgg tctccttgat gcccgacagg gcatcctgaa gataggcatt gaggtccgcc
    27301 acacttttgc ggatctcggc gtagtacccg tgcacccgtc gggtaaacca ggcggcacag
    27361 acgatcagga tcggaatggg cagcagcgaa agcagcgcga gcttccagtt caggaaaaac
    27421 atcatgatcg tgatgccgac cagggtcagc gaggccgtca gcaatccctc gagcccgtcg
    27481 acgaaaatcc gttcgacatg ttcggtatcg ctggtcacgc gggtcatgat ctcgcccgtc
    27541 gagcggttct cgaaatagct caacgacaag cgttgcagcg ccccgaacac ctgcatgcgc
    27601 aacgcgtgca cggccttctg ttcgagcaga ttattgaacc ggacccgcat cgagctcatc
    27661 aggttccgca ggacatagga gccgaggagc gctcccaaca cccagggcaa catctcagga
    27721 cgcttcgact ggatcacatc gtcgatgacg attttgacga gatacggcgg aaccagttcc
    27781 agcgccgtgg cgagccccgc gcacacgaag gtcaccaccg ccagcatccg gaacggcttg
    27841 aggtaagaca gaacgcgtag aagcgagctc acggcgactc gacgatccgg gacggatcag
    27901 atgacactgg ccggttcctt ctgccaatac ttctgaaatt catccatctg ggtgagggtg
    27961 gaaaagtcgg tcatgcggtc gatgaacagc ttaccgatga gatggtccag ctcatgttga
    28021 atacagacgg cgtacaagcc gctggcttcg aaatcgaacg gcttcccatt ccgatccagc
    28081 ccggtcactc gaacggtcga gggacgcgtc actttgcctc gcaacccgtc gacgctcaag
    28141 cagccttccc agccttcaac ctgctcgggc ccgtaaaact ggatcgacgg attgatgagc
    28201 accgtcttgg gaaaaccgcc ttcaccgggg caatccatca cgacaagttg ctgcgatcgt
    28261 cccacctgcg gagcggctaa accgatcccc ggttcgtcgt acatggtttc aaacatgtcg
    28321 tcgataagtt gctggaacgc ggcttttttt atctccgcct gggtcagagg cagggccttc
    28381 tgccggagaa tcgggttacc gagtttactg attgccaata tcgccatcct gcaccttttc
    28441 tatcaatacc atcgcgcgag cgactcacgc cgcgcaaccg gtctcttcat tacctaccac
    28501 ggctttttga ctctccgcaa ccgccgaaat ccagtcgtca accggcccgt ttccggctct
    28561 tcggttccgg catttcaaac gtctctttcg actggttcca ccattcaatc cattcctgcg
    28621 cccaagcctg gcgatcctgc ttgttggagg catcccaatc gtgaaactcc gtctcatgcc
    28681 gggtcaaggt ccagagcgac tgcacggcag acagggccac gaaccgttcg tcgtccccca
    28741 gcagatcgat gaggggctga atcacctctt tccgacgcac ataccgcgat tcaaccgccg
    28801 ccgccttccg aatcgacgca ttggcatctt tcgccatcgc ttcaatggcg ggcgcggcct
    28861 gcatcggatc gagccgcact gcgacccgga gcgcatgctc acgaatgcga ggcagttcgg
    28921 tccccagctt cgccgtatcc agcagagccg gcacggcagc cggagacttg atcatggaga
    28981 gggtctcaat ggcattgtac cgtatccgcg ggccgggcat ggccagcgcc ttggtcacca
    29041 ccggcaggac cggctctccc agatgcacga attctcccat cgcccagaat tcttgttttc
    29101 cctccaacaa tggcaacagc gcctcggccc gctgcagttc ctcaggcgtc agggccttgg
    29161 tattaggggc caccgagaca tccgggaccg gctcggactt gggcggcgcc tcatacggca
    29221 tctgcaccgg ccacacggta gcgtcggacc aggcttgatc cacggtgatt cccaccaagc
    29281 ccaggcacac caggccccga accaacaatc gaccgaccat ctgctgcctc gctcccatca
    29341 taatagtgac ctacccccta cagcatgacc attaatgtgg aaccaccggt tcctactctg
    29401 cttcgaccgc gcgcgtgctc gccgtgcgac gatgcacgac ttcatacaag accggcagca
    29461 caaccaggat caacaccgcc gcagtcagca taccacccac caccacccgg gccagcggtt
    29521 gttgtgcctg cgagccgatc cctgtggcca acgctgcagg aagcaacccg atggccgcgg
    29581 cgagcgtggc catgagaatg ggacgcatct gcacatccgc ccccttcaag acggcctctc
    29641 gcaaatcgag cccatgccga cgaaaatcct caatgcgtga gattagcagg actcctccga
    29701 gaatcgctac tcccagcgtc gaaatcaccc ccaccgctgc agagatgctg aagtgggttc
    29761 cggtgaagac gagcgagaaa ataccgccga tcaacgcaaa cggcactgtg accaacacca
    29821 gcagcgcgtt cgtcagagag ttgaaggtcg tgtagaggag aaacaggatg atcaccagac
    29881 tcaccggcac gatcatcgtc aggcgttttt gttcggccgt gagttgatcg tactgaccgg
    29941 cccattcaag ccgataccgt tccgggagcg aaaccgcttg agccatcttg gtctgggcct
    30001 ccttcaccgt gctctccagg tcgcggtcgc ggacgctgaa cttgatcgga atataccgtt
    30061 cgttgttttc ccgataaatg atgaatgcgc cggtctgagt tctgatcgtc gccacctgtt
    30121 tcaggggaat ccgcgcaccg tccggcgtat tcaccagaat attgccgatg gtctcggcat
    30181 cccgtcgaaa ctcgggaaga aaccgaacca ccagatcgaa caggcgctcg ccctcgtaga
    30241 cctgcgtcac cccctgaccg cccaccgccg cctgcaccac cgcgttgacg tcggacacgc
    30301 gcaacccata tcgggcgctg gcttcccgat ccacctggat cagcagattg ggctgtccta
    30361 ctaaccggaa aattcccaaa tccttaacgc ctcggacccc cttcatcacc gcttcgactt
    30421 cgacggcctt cgcctccatg gtcttcaggt cgttgccgaa taacttgatg gaattctccc
    30481 ccttcacacc cgacatggcc tcttccacat tgtcttgaat gacctgggag aagttgaaga
    30541 cgatcccggg aatgaccttc agccgcgcct cgatctcttc caccaactgg tccttgctga
    30601 gtcccggccg ccactccttc tgcggcttca gatttgcgag gaactccgcg ttgaagaaac
    30661 tcgtggggtc ggtcccgtcg tcgggtctcc cgagctgaga cacgatcgtg acgacctcgg
    30721 gcgactgccg gaacatctgc cggatttctc cggtcaagcg ggcggcctcg tcgtaagaaa
    30781 tatcgaccgg catggtcgcg cggacccaga ggttcccctc ctccaacgcc ggcatgaatt
    30841 ctccaccgat gaattgcagc gccccgaatg ctccgaccag cagcaacagc gccgcgccga
    30901 tgactttcac gttgtggcgc aaggcccatt gcaacacggc cagataggtc cggcgaatcg
    30961 cgccgacaaa ccgcgtatct tcttccttga ccactccggt caagagcagc gagcacagca
    31021 ccggcgccag cgtaaaggcc atgagcagcg cgccgaagag ggcaaagcca taggtaatgg
    31081 acatcggcgc aaaaatcttc ccgggcacgc cggtcatcgt gaacagggga atgaacgcca
    31141 ccacaatgat cgccgtcgaa aagaaaatcg gctgcccaac ctggcgagcc gcgcgcacga
    31201 tctgctgcgg cacagtcagg cctagacggc gatcgtggcc gagatggaaa aagatacttt
    31261 ccaccatcac cagggtcgca tcgacgatga taccgaaatc gatcgagccg agcgaaatga
    31321 ggttggcgga ctgtccgatc atcaccatca tcgtgaaggt gaacagcagc gacatgggaa
    31381 tggtcaacgc cacgatgagc gacgcacgga aatgaccgag aaacacgaac agaatcacga
    31441 agaccaggac catgccgctg atcaaaatat cggtgaccgt ttcaaccgtg gtatggatca
    31501 gggccgtccg gtcgtagaag gtcttgacct tcaccccttc cggcaatttc cacttgttca
    31561 gttcctcgac cttttcccgg acccgctcca gcacggacag cgccttgtat ccgcgctgca
    31621 agaggatcac cccttccacg acgtcgtcgt tgtcgtcgat gccgaccttg cccaaccgca
    31681 cccgatgacc caccgacacg gttcccagcg tcttgacgaa aatgggggtg ccttcctttt
    31741 cggccaccat cacattttcg atatccgcca aaccgttgat caacccgagg ccgcgaatgt
    31801 tgtagttctg ggcgccgacg gtgaggtagt tgccgccgac gttggcgttg ctgttggtca
    31861 aggcctccat gacctgcgcg aggctgactt gataactgat caacttcccg ggatcgatat
    31921 cgacgtgata ttcctgggtc gtgcctccga aggccgtcac atcgatgatc cccggcacac
    31981 gccggaactc tcgccggacc tgccaatcct ggatcgtctt gaggtcggtc agactcgtct
    32041 tcccgtctcc ggccagttca tagcggtaga tctctgcaat cgcccaccag ggcgacaagg
    32101 ccggctgcac attttgagga agactgaccg tggccaggcg attgatgact tcctgccggt
    32161 cgaagaagta ttgcgtcccg aagtcgaaat agatcttcac gtcgctcaac ccgaaaatcg
    32221 acaaggagcg aatatcggtg agacccggca tgccgtttaa ggcgacctca atgggaatcg
    32281 tgatttgccg ctcgatctct tctgcggacc agccgggaaa ttgcgtgatc acctccacca
    32341 tcggaggcga cggatccgga taggcgacga tgtcgagttg ctggaaggca tagagcccgc
    32401 cgaagaggag actgaatccc agcgcacaga tcagaaaccg ctgaacgagg gagagctcga
    32461 cgatacgaac aatcatgagg cgggatacct ctgggagagg cgagagcaca tgatcatgac
    32521 ctcgatcatt acttcgcctc ctgtcccttc accagcaccg cgcccttgat gacgatgcgc
    32581 tggccggatg tcagtccctc cagaatgcgg acctgcccgc tggacacgtt ggagaccacc
    32641 acttcgcgtt tctcatagtg attgggggcg tcgaccaggt agacgaaatg tttgccgtcg
    32701 atctcgacga ccgcttcctt gggaatcgtg aggaatggcg tggcgtcgcc gacgtccaac
    32761 ctcagccgcg cgaacatctc cggtttcagg cgctgttcac gattatcgac ccaggctcga
    32821 actttgatgg tgcgggtgtt cgggtcgacg acatctccga tggaggccac gacggaggcg
    32881 aagtcggtcc ccggataggc ttccacgttg atgcgtccga cctggctgac tttcaccaag
    32941 gcgaggtccc gttcgtagac atcggccacg acctgcaacc gatccagatc ggccaccgtg
    33001 aacaacacct ggccggcgtc gcttccgaca gactgtcccg gagtcacggc ccgttcgacg
    33061 acggtgccgg tcaagggact cttcatctcg aagcgcgagg taatacgctg ttgggcaagc
    33121 ggtttctcca gctcttgagc aggaatgcgc aacgacaaca agcgctcttt tgcccgccga
    33181 aattccgcgc gcgccttgat cagatcgttt tcagcctgct tgagatcttt caaggccaag
    33241 gccttggtgg catacagttc cttcgcaagt tcctgcgccc gcgtggaata ttccatctcc
    33301 gaggcttctt tcacaaactc cgaataggcg gcggtaatgt cggaactgtc gatcaccagc
    33361 aaggtcgccc ctgcctccac gcgatcgccc aacttggccc gcacctccac cacccgtcct
    33421 tgcaatggag aggaaatttt cgaataccgg tcttccccgt aggccacctt tccgaccagg
    33481 accagctcgg gctggatcgg tttaaaatcc accacggccg tctcaatctg tccgatggac
    33541 tgcggcggag gctggacggc aacatgttcc ggggtcacgg gaggcttcga agcggctggt
    33601 tcttgcgttt gactgcaggc ccacaacgac agcgctacgg ctacccctgc gaccgacctc
    33661 acgccaatga tcatgacgta atctcctgcc cgaccgaact ctccagttgg aacaggttcc
    33721 gctgatagga aaagagggcc tcgatgaaat tctgctgaat cgtacgggac gtccgcgcgg
    33781 catcgagaag atccaggatg gtggctcctc cccgttcata ggcgcgctcg acgatggtga
    33841 aggtggagcg ggcatcttcc agcactcccg ccagataggc ttccaccagc cggcgactct
    33901 ggagcagatt ctggtaggcc acatccacct ggttttccac ctggttgacc gtgcgatcca
    33961 attccgcctg ggccgactgt acgccgactt cagcctggac gatcccgccc tggttacgat
    34021 tgaacaacgg caggggcaca ccgagattca ggatccccat ctgctggtta tcgggaccac
    34081 gcgagccttg caccgcatag ccggcgccga tcgtcacatc gggaacccga aaggccttgg
    34141 ccagtttgag atccgcctct cgttgcaaca aggccgcccg ccggctcttg atatcgggac
    34201 ggacatccag ggccaccgac ctgagccgtc ccatgtcggg atccacccgc cggtagtcca
    34261 tttcggtcgt caactccagc ctcgacgcag gagagagacg cagcagttgc cggagatcgg
    34321 ctctggcgga ctcaccctcc tgaatcgcct cgatcacctg cgcttgaaaa tcgaccacct
    34381 gcagacgaat acgaatcaga tcgacttcgg cgatataccc ttttttaaaa cggatcgtgt
    34441 tgacgtctaa aatacgcgca aaccggtccc ggttttcttc ggccagagac aaccggcgct
    34501 gcgccaccag cactcgatag taggcgtcct tgatcgtgaa tcccaactgc cggacggcat
    34561 cttcaaaatc cgcttccgcc gactgcaccc cgaagccggc actttcgatg cggtatccgc
    34621 gcttgccggc caattcaaac aattgctgca cctgcatggt gagcgcgccg gttttggaca
    34681 gcgtattgcc ctgcgtaaac gaggccacat ttcccagctg cgccaccgga ttcggaaaga
    34741 gccgtgccgt aatctgctgc cccttgctgg actcgatccc gtacttggcg atcagcaaat
    34801 ccaggttctg cctcagaaac agggccatcg cctcggacaa gttcaggcgg accaccggcg
    34861 gagaaaacgg aaccgtcggg ggtggagttg attcagccgc ctgcgcaatc gcgccgccga
    34921 tcacgaagaa tcccagcagg ccgatcgcca gccaggcacg acagaccagg gccgataggc
    34981 acgggcctac ccgcctgcaa tctctgtagt ggagcatgtc gtcacgcatt gtgcaggagt
    35041 atcgaaccat cgaggacagc gcgcgcatta tactgattct tcagtgtttt tgaaacaaga
    35101 cccgcggcac tcgctcattg cattttcagt tccgtccagg cccggtcata gagacggatg
    35161 cccctgccga catccttcat ccactccaat cgatcgagtt gatcgagcgg cggatacacc
    35221 gcgggattgt ccctcgtcga ggcattcacc aattcacggg ccggccggga agcggaggca
    35281 aaacgcagcc gctccgacgt gcgcgccgcg acctgaggct ccattaaata gttgataaac
    35341 cgcatggcta actgtttctg aggcgccgtc cggagcacca ccagacagtc ggcccacaag
    35401 gttgcccctt ccttcggaac gacatagcga atggacggcc gctcggccat ggcccgcgcc
    35461 acgggtccac cccaggcatg cgccaggacc acctcccctg aagccaacaa ctgatcgtaa
    35521 tggtcgctcg tataggtctt caccaaaggt ttctgccgca gcaaccgctc ctttgccgcc
    35581 tcgatcacct gcggatccgt gctgttcatc gattgcccca tcgaacgcaa caccgcaccg
    35641 aacacttccc gctggtcgtt caacatgctg atgcggcctt tgtaccgagg gtcccatagg
    35701 atcgcccaac tgtccggcgg cgtcgggatc accgcagaat cgtacccgat ccccacgctg
    35761 ccccacagat acgggatcga gtaccgccgc tccggatcga aaggcagcgc ttgtaaatgc
    35821 ggttccaaga acgacgcgtt gggcagcgcc tgctggtcca attcactcag cagcccttgc
    35881 tggatcatga tggccaccat gaaatccgac ggcaccgcca cgtcataccc cgtggccccg
    35941 ctctgaagct tggccagcaa ctcttcattg ctgctgaagg tatcgatcac caccttcacc
    36001 cgctcgcgcc gttcgaactc ctggataatg tccggcccca catagtcgga ccaggtgaag
    36061 taatgcagga ccggtcgaga ctcggcagga tcactccctg caccttggcc gcaggaaaac
    36121 aacgcccctg ccagccaaag cagacacagc ccccaggggc tcattcgtcg gagaggagtc
    36181 cccactcaga cctcccggcg ttgaagatga agggagaggg cgacacaggc catggaaatg
    36241 agcagcagca gcgcggagag tgcattgatc tccggcgtga caccggactt gatcatcgaa
    36301 tacaccttca acggcagcgt cgtcgcaccg ggaccggccg tgaagaacgt caccagaaaa
    36361 tcatcgagcg agaccgtaaa tgccaccagc accgcgcccc agatcgccgg gcgcaagagc
    36421 ggcaacgtga catgccggaa gacctgccag gagtcggcgc ccagatcagc cgccgcctcg
    36481 cgcaacgccg gatccagctt gcgcaaccgc gtgcggatca taatggtcac cagcggaata
    36541 ttgaacacga tgtgtcccag aatgaccgta gtgagaccga gcggcatctt gagcatcacg
    36601 aaaagcagca tcagcgccac gcccatcatc acctcaggaa tgacgagcgg cagcaccagc
    36661 ccgccctcga tgatctgctg ccggcgaagc ggcatacgct ccatgccgag tgccatcagc
    36721 ccgcccagca aagtcgcgcc gatggtcgag atcacggcaa tgagcaggct gttcgcggtc
    36781 gcagccagca gcgcctcgtc atgccagagg gcggcatacc acttgagggt cgtcccccgc
    36841 caggccatcg acaggtgggc ggcattgaac gaatagagca cgagcacaag aatggggccg
    36901 tagagaaaca gcatcgccag accgctcacg aggctcaggc cggtcgatcc tcgcctcatc
    36961 gtccctccaa cggcccggca gtccgggcgg catggcggta atacagcgcc accgcggcca
    37021 tgacgatcgc catcagcaca aaggacaccg ccgatcccaa cggccagtcc cgcacgacca
    37081 gatactcgtg ttgaatgaga ttgccgatca tcatgcttct cgccccgcct agcagatccg
    37141 gcgtgataaa cgagccgatc gacgggatga agaccaggac acagccgccg acgatgcccg
    37201 gcatcgtgag aggaaccacc accctcgtga atatcgccca gcgcgaggcg tacagatccc
    37261 aggccgcctc caccaggcgc ggatccagcc gttccacggc gacatagaga ggcagtacca
    37321 taaagggaag gtacccgtag accagtccga tcacgacggc cgtcggcgtg tagagcaatt
    37381 caagcggggc gtccactatt ccgagagccc gcaacaggcc gttcacaagg ccgtcctggc
    37441 gcagcacaat catccaggcg taggttcgca cgagaaagtt cgtccagaac ggtaacatca
    37501 ccagaagcaa caacaccggc tgccaccggg ccggacttct ggcgatcatg taggcgagcg
    37561 gaaatccgag cacgaaacag atcgccgtgg tgagactcgc gtaccagagc gactgcccga
    37621 ggatcttgcc gtacagcgga tgccacaggt ccagatagtt cgccgccgtg aactcccaca
    37681 caatgccgcc ataggttccg cgtgaagcga agctgatcgc aaacaccaag gccagcggca
    37741 cgagaaaaaa caggcccatc cacaacagac cgggagcggc cagccaacag gaacggcagg
    37801 tggattgacc aggcgcagga ggtgaagaca ggtcgtgaga gggcatggtc actcgaagaa
    37861 caaccgcgca tcagccaggg accagtgaag aaacaccggg tctcccgccg cgaacgccgg
    37921 gagcgatgtg cccgaggtca tcctcgtttc ccagaagcga ccgggagcga ctcgaaccaa
    37981 atacttcatc tcgctcccgg caaacaggac tttctcaatc cggccggaga gcaccgggtc
    38041 ggatgtcgac ggccgttcat gcgccatgcg gatgtgctcc ggccggagcg agagcgtggc
    38101 tgaacggccg gcctgttccg tggaagcggt tcgcaccgtg agcggcgcct gttcggcatc
    38161 gtccggccga aacacgcgat cctccccgac cgaggccccg atcgtacccg ttagctcatt
    38221 cgacacgcca agaaatcggg acacaaacag attacagggt cgctcataca cctcgcgcgg
    38281 gcttccgacc tgccacatgc gcccctgatg catgacggca atgcggtccg acatcatcat
    38341 cgcctcttcc tgatgatgcg tcacacagac acaggtcagc ccggcctgct cctgaatgga
    38401 tttcagctca acctgcatgg cctgccgcag ttgttgatcc aacgcggcta acggttcatc
    38461 gagcagcacc acagccggct tgttcaccag cgcacgggcc agggccacac gctgttgttc
    38521 gccacccgat aattgattcg gcatccgtcc ttctttgccg agcagcttca ccatcgccaa
    38581 ggcctggcgt acctgctcag caatgagtgc tgcggacacc cgccgcatct tcaggccgaa
    38641 ggccacattc tcgaagaccg tcaggtgggg aaacagcgcg taggattgaa agaccagatt
    38701 caccggccgg cgattcggcg gcacgccgag catcgactgc ccgtcgatca acagatcgcc
    38761 ttcgtccgga tcgtcgaagc ccgccagcat gcgcaagacc gaagttttcc ccgcgccgct
    38821 cggaccaaga atggaaaaaa actcgccccg gcggacctgg aaactcaccc gatccacggc
    38881 cgtgaccgca ccgtggcggc gcacgatccc gcgtatatcg atcgttggaa gtgttgagga
    38941 aagagactgt tcagaagcgg tgaacggagg aatggacgac gtggccatcg aaggctgaat
    39001 tcaaaccgcc gcgccgacag ccacggctca atgctccaaa tcgactttgg aactgacatc
    39061 gcgcaggtgt ttgcgcaccc gcacccgatg ctcatgtttc cgcaagagtt cacggcgaat
    39121 gacgtcgcga tcctccgcca caatgcgcac aagccggtcg ttatcttcgg catggttccg
    39181 cacgagctcg gagaggcgct gcacctgccg ctccagccgt tccagccgcc acaccatttc
    39241 catcatcgga ttcgcggctt cgatatcggg cttgacgacc gcctgacggg cattctgttt
    39301 cttcatgtcc gctccttcgc attgagcccg accggatgtc ggagccgcac gttgagtcgt
    39361 agtatcgtcc gagatcgagt tgaagtcaat cattctgcat cctttctatt catccgtcgt
    39421 cctgctacaa tcggcccgtc tatggctaag atcttcacca tggtgctggc gggcggcaag
    39481 ggcgaacgcc tgaatccgct taccgaacaa cgtgcaaaac cggcggtgcc gttcggcggg
    39541 aaatatcgca tcatcgattt cacgctgagc aactgcctga actccggcct gcggcagatg
    39601 gccgtcctga tccaatacaa atcgcattct ctcgaccgtc atatccgcac ggggtggaac
    39661 attctcaatc ccgagctggg cgaatacatc gtgtctgttc ctcctcagca gcgtatcagc
    39721 gaagaatggt atcgcggcac cgcggatgcc gtgtatcaaa acctcttcct gctcgataac
    39781 gaccaccccg agttcctact cgtgctggcc ggtgaccaca tctacaagat gaactatgcc
    39841 gacatgtaca acctgctgat cgaaaagcac gcggatgcgg tggtcggcgc gattgaaacg
    39901 ccgctggcgg atgccaaccg gttcggcgtc attgcggtgg ataaagaccg gcggatcctg
    39961 agcttcgacg aaaagccgga gaagccgatg cacatccccg gcgacccgac ccaggcgttc
    40021 gtctcgatgg ggatctacct gtttcgcacg gacgtcgtac gcgagcaact gatccgggac
    40081 gccaaagagg gcaccaagca cgacttcggc aagaacatca ttccgcgcat gatcaaagag
    40141 cagcgcgtct atgcgttcaa gttccaggac gaaaacaaaa aagccgtgaa gtactggcgc
    40201 gacatcggga cgctcgatgc ctactgggaa gccaacatgg atctcgtggc cgtcgatccg
    40261 ctgttcaacc tgtacgatca gcaatggccg atccgcacct accagggaca attcccgccc
    40321 gccaagttcg tcttcgccca ggattttcag ggaggccgca tgggcgtggc gctggactcc
    40381 atcgtctgcg gcggctgcat catttccggc ggccgcgtgc agaactcggt gctttcgccg
    40441 aacgtccggg tccacgacca tgccgacgtg cgcgagtccg tcgtcatgga gaacgtggtc
    40501 atcggcgaac aggcccgcat caggcgggcg atcatcgaca aagacgtgat catcccgccc
    40561 aaaaccgtga tcggcttcga cccggttgcg gatcgtcaac gtttcaaagt caccgattcc
    40621 ggattggtgg tcatctcaaa gggaatgaaa ctgcatgccg ccgtcgattc atccggttga
    40681 tctggtcgca acgctccgcc aaagaaatct cactccggcg attatctttc tcacatcgag
    40741 acgggcctgc gacgaagcga tgcagtcgtt cgagcgcagc caggtcaccc tgcccaagac
    40801 acagcaggaa cagatcgccc gcgtcctggc ccaggtcacc gaacaatatc ccagcatcgc
    40861 agaacacccg ttgatcgaca cggttcaacg gatcggtgtg gcggcgcatc acgcgggcca
    40921 tctcccctcc tggaagatcg ccgtcgaaga actcatgcgc accgggtctc tggatgccgt
    40981 cttcgccacc accaccctcg ccgccggcgt cgactttccc gcccgcacgg tggtcgtgac
    41041 acaatccagc atcaggaagg cgcgcgactt taccgacttg accgccagcg aaatccagca
    41101 gctggccggc cgggccggcc gccgcggcaa agacctggtc ggattcgccg tcatcacccc
    41161 gtcgccctat atcgatctca ccgtcctgac caaagggctc acgggacaac cggaagcgat
    41221 tcatagccaa ttcgtcatca gctacccgat ggtgctgaac ctgctcaaag cgcacccgcg
    41281 cgaacagatc caatccattc ttgcgaaaag cttcgcccag tttcagttga atcagcgcac
    41341 ggccgtgctc gaaaccaaac tcgatggact gcgcgtcgag atggaaccct tcggtccgcg
    41401 cgtctgctcg gattggatca cgcagtggca ggtattcgat caggccagga aacggaagaa
    41461 accgcgtggc ctgtcggcca ggcaggaatc gcccgatgtc gccgcgcgcc tgcattttct
    41521 cacgccggga cgcgtcgtgg ggctgatccg gggacgcggc atcatcctgc gccagtatcg
    41581 aagcaagggc cagcatgcgc cgatggtgac gttgctgcgg gacggcggtt ccctcaatga
    41641 atgccccgcc tcgatcgtga cgcaggtgtt cgatcgcacg ttcgactgcg aggaaacctc
    41701 cgcctatccc tggtgtactc cgcagacact ggagcagctg acacaccacc tgctggatct
    41761 gccgccgcgt ctcccgattc tgccgattct gactcatcag cacgaagaga ttccgctcga
    41821 cagttcgatc atccaaaccc ttggcgattt cgcctgcccc acctgtccct cgcgtcaggc
    41881 ctgtcaacgc gatttcccca gggcctcacg ggtccgccag gaaatccagc ggcacatgaa
    41941 atcgattcaa gcgctccaga ccagcttgtg gcaccggttt caagagcgca tcgatgtcct
    42001 cgagcgcttc ggctatctca acgccacggc acagctcacc gccgacggcg agtgggcgcg
    42061 cttgattcgc atcgaccatt ccctcctgat tacggaactg atccgggccg aggccttcgg
    42121 cgccatcgat ccgccgctgc tcgcggccgt catggccagt atcgcgcatg acgacgatcg
    42181 tcccggctca tttccacgcg gcagcgcggg gctcgtcacg ctcctgttcc aggtccggaa
    42241 gctggcagaa agtcttgtgc cgcacgaggc gccgccgatg cttcgtgccg atgtcgccgc
    42301 cttgaccgaa cgctgggtcg cagaccagac gctgacctgg atcggcctgg cccgcctgac
    42361 cacgatggcc gagggcgaca tgttccggct gttcgcgcgc acgatcgagt ttctctctca
    42421 attgaagacc ctcaaagcga cccacccctc gttggccgaa tccgccgatc gggccatcgc
    42481 aggcatgcgc cgtggcgtcc tggaggaact accgtgagcg tgtcgttcgc aagaggattt
    42541 ccgacgggcc tcgtattcac acgtttcacg tttcacgttt cacgcatcac gaggcctcta
    42601 tgacgacgca cgaactcgaa cagctactgc tgcagcaacc ggtctctctg ctgcaccgat
    42661 tggcgcgtgg acggatcagc cggcattttc gttccggcaa acgcaagctc atcgatctgt
    42721 tgctccaggc ttccgctgaa aatcgccccg ggctggagtc cgacctggct gccctgatcg
    42781 aggaacaatc acacagacag caggctgtgc cggttgagca agctcccaaa ccgaaggtcc
    42841 acccgagacc ggctgcagcg tct
//
LOCUS       DAORRQ010000039        42301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_60_length_42301_cov_29.080268, whole genome shotgun sequence.
ACCESSION   DAORRQ010000039 DAORRQ010000000
VERSION     DAORRQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 42301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60_length_42301_cov_29.080268"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PK224_13045"
     CDS             <1..940
                     /locus_tag="PK224_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB16714.1"
                     /translation="DLLSTMMSPIGSFGDKLKVLRLRRDALRRQLCGQAGGGERTTLE
                     ALQVYGFSPAMQQRFFQPFLNGVFLDQALSTPCRLFELVWAAFSRGDIALPQQGMGAI
                     ASQLAATLPTESIRLNAAVSHLDGTTAVLASAERLAADVLVLATDDATAATLRGDSRA
                     AAPGRDAICLYFDAPAPPVRGPWLILNGDGQGPVGTACALSEVASGYAPLGRSLISVS
                     LSGQPSLAGGDLQQAVRRQLREWFGEEVHVWRHLRTDHIKRALPPIEVLTAQASGASP
                     RVRPGLYQCGDYCETGTLDGALLSGRKAAEALLADH"
     gene            966..1433
                     /locus_tag="PK224_13050"
     CDS             966..1433
                     /locus_tag="PK224_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB16715.1"
                     /translation="MNGRGARVTNHFGWRWLLAATMVLLSCPLPSALADPSSQHQLQA
                     LAGQGDANAQWMLGQALLTGSLGETDEAEAVRWLQLAADQGHALAQRDMGMLYEQGQG
                     VTQDVLEAFFWYSLASRQDSSRARLRRDALAAMLTAEQHEAIAARLKGWRPRK"
     gene            complement(1451..1942)
                     /gene="def"
                     /locus_tag="PK224_13055"
     CDS             complement(1451..1942)
                     /gene="def"
                     /locus_tag="PK224_13055"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRB16716.1"
                     /translation="MAIRPILQYPHQALKSTNAAVAPSDPAVQAVVQDMLDTLAASPG
                     VALAAPQIGHAVQVIVVDVSRKKGEGGHGLVVLLNPVILSLEGKKSVREGCLSVPDYT
                     GHVLRYEQALVEGMAPDGRVVTVSASGFEALAFQHEVDHLNGMLFLDRIQSLSTDLFR
                     RKK"
     gene            complement(1945..2493)
                     /locus_tag="PK224_13060"
     CDS             complement(1945..2493)
                     /locus_tag="PK224_13060"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRB16717.1"
                     /translation="MNHFAIVTAFGQDRPGIVALMADSLYRLGCNIEDTCMTRLRGEF
                     TMMLMVRLPEGIAAEELGRRLTPYTTPLDLAVLCRAMPDQAATRQTALEVPTFMLSVY
                     GADHPGIVAQVARTVAQHNGNITDMNTRVVGSGERPIYVMVLEIQLSEAQQADQLRQA
                     LEQLKPLLGVDLTFRLLESVTF"
     gene            complement(2609..5107)
                     /gene="hrpB"
                     /locus_tag="PK224_13065"
     CDS             complement(2609..5107)
                     /gene="hrpB"
                     /locus_tag="PK224_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HRB16718.1"
                     /translation="MVRLPIEEAIPALRRTLATGCAALLTAQPGAGKTTRVPLALLHE
                     PWLAGQKLVLLEPRRLAARAAAAYMAAMIGEPVGKTVGYRIRHDTRVGKDTRIEVVTE
                     GILTRLLQHDPSLAGYGLVVFDEFHERSLQADLGLAFARESQRLFRPDLRLLVMSATL
                     DCAAVTRLLQDADTISCEGRLFPVTTQYLDRPIEGHLEPAVVRSIRQALARDEGSLLV
                     FLPGMAEIRRVERQLVEASLGPNILIAPLHGELPQSEQEQAILPAPTGRRKIVLATSI
                     AETSLTIEGVRVVIDAGLMRVPRFDPRSGLTRLDTIRVTQDAADQRRGRAGRLEAGTC
                     YRLWTTAEQQALLPRRPPEILEADLAPLALDLAEWGVIETAELSFLDPPPTGTLAQAR
                     DLLRQLGALDAQGALTAHGRRLAHVTVHPRLAHMMVTAVPIGLGAHACDLAALLSERD
                     ILQGGPGWRNADLRIRVEALRGTRDHLAGATINRAGCERVQRASEQWRRQLQLTPSSV
                     DGTDHTGTLLALAYPDRIAQRIAGSEGRYRLANGRGAAFHTVQGLSQEEYLVVAQLDG
                     TGDWARILLAAPVGLVDLQHHCAEQIQAVDLLEWDDRSESVRARRQRRLGQLILDDRA
                     THDPDPVQVTAALLFGIRREGLTCLPWAKELQQWRARVGFLHRIDPTWPDLSDGALIN
                     KLDAWLGPFVSGLTSLAQVRRIDLQPPLESLLTWQQRQELPRLVPTHLTVPSGSHVRL
                     DYDQSETPVLAVRLQEMFGCQETPRIAGGRVPVMVHLLSPAGRPVQVTKDLASFWRST
                     YQEVKKELRGRYPRHHWPDDPLTAQPTNRTKRRT"
     gene            5142..6278
                     /locus_tag="PK224_13070"
     CDS             5142..6278
                     /locus_tag="PK224_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16719.1"
                     /translation="MKSDGELLQSIPKVNMQKGASAIVAYTSPFRLIFRDAKDSWAPS
                     LEQINQNGYDSIKLHRLSTSIDIGLPQPFCCHVTFDGALLLPRVKQFWPIEKAVAAFN
                     RVLGEFMLGGVYFESIGPTDIDQAILYPTGYYRPLGQAKGLPGQLRYGLQAKMASPLH
                     SILLYQPEHLLAKDLHVARKRGAALSSKFETLSLEFLVQGISAFATHDWAGALSHLWI
                     SLEQVVSCLWKDQVIVGGVQPKFPIRNRKEFLQDHRTWVTSARLEVLFQRGILSEIAY
                     RKLNNARKARNELAHLGRAPSRESASAALEGLFEVIESSDKSTSLAEIINTIKSRDPL
                     ERYYSMSGMGESGEGGLWLGPLPPIPGEPEWGVKEYERVYDKKN"
     gene            6389..6973
                     /locus_tag="PK224_13075"
     CDS             6389..6973
                     /locus_tag="PK224_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRB16720.1"
                     /translation="MMVLPQTCERALLERFLRAEAMALWAVRAAQTQNLPRHVQTFLQ
                     RHETDEQDHLRQFEGMLGTTSQRAPTLPTVPSQWEMLAVLLFGYEALGLEFATLLATV
                     RPDLADILEDEQVHVGFFEKELRAILAGGESGAQQAREAARTWWKKLPRTVDRYLGDP
                     SLAPYRTELRHHILSIIQERFIALGLLPAGQAGR"
     gene            complement(7006..8322)
                     /locus_tag="PK224_13080"
     CDS             complement(7006..8322)
                     /locus_tag="PK224_13080"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB16721.1"
                     /translation="MSKSIVNASVATMLWSWGVWTGGAFGQSLLTGTPLGEAPTPVFS
                     AQTPGLEGRSSSDPLWTDALWHADEKGINRFFPQDGFLRVRGWVDGGYTYNASNPRSN
                     FSGPYNAIDRDIPVLNQLYMIVEKPLTATGSNWGIGGRLDFMYGYDYFLTQSNGVERR
                     ENGAQRWNAQGQHYGLAMPQAYAEIGNQTFSVKVGHFYTIIGYEGVPSINNFFYSKSF
                     AYQFAGPFTHWGGLATWHLNDRITLQGGLVNGWDSLDRTKDSTTGLASIKYTAPENLW
                     SLSFSMVTGNEPSAVATQFETRTRYSLIFTAHPAERWEYVFHHHYAYQNQGKVDGGTA
                     RWYGIDNYLYYALTDQWRAGLRFEWMRDEAGTRVGGNPVRDNPNLGPFAGSFYSLSAG
                     LNYRPHPNVLIRPEVRADWFDGQRSPYNDGLNKNQVLAAINGTLQF"
     gene            complement(8602..9570)
                     /locus_tag="PK224_13085"
     CDS             complement(8602..9570)
                     /locus_tag="PK224_13085"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRB16722.1"
                     /translation="MRDYLDFEKPIRELEEKIEKLASAESPKSGTQDEIRKLRTKLAQ
                     VEHQLYTTLTPWQRTQLARHPQRPTTLDYINELSREFLELHGDRSFGDDRAIVGGFAR
                     FNDRSVMIIGHQKGKTLKERMQRNFGMPNPEGYRKALRLMRLAEKFGRPIITLIDTPG
                     AYPGIGAEERGQAEAIARNLFVMSRLSVPIISVVIGEGGSGGALALGVSDRILMLEHS
                     VYSVISPEGCAAILYDDPSKVPDAAASLKMTAQDLVGLGIVDEVIPEPLGGAHRDPRA
                     MCDRVAKTLANQLYALVELPTDQLIAQRDQKFRKIGVVGGLVPVGA"
     gene            complement(9608..13060)
                     /locus_tag="PK224_13090"
     CDS             complement(9608..13060)
                     /locus_tag="PK224_13090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRB16723.1"
                     /translation="MASQFVHLHLHTQYSLLDGANQIDPLMQQVKSFGQPAVAMTDHG
                     NMFGAVEFYRKAKEAGVKPIIGCEAYMAPGSRLEKNSHLAHNDYYHLILLATNLKGYQ
                     NLIKLVSKAYLEGFYYKPRMDKEILQQHHEGLIGLSGCLSGEVAYLIGQKDLEGATKA
                     AGEYREIFGKDNYYLELQANGLEHQRIANDGLLEIHKKLGIPLAGTNDCHYLKKEDSR
                     PHDLMLCLQTGKTINDPNRMKFDTDQLYVKSTEQALSEFKEMPTAVSNTVKIAEACTL
                     DLALNKTYLPQFKVPEGYTRESYVEQLALEGLAARLKERPSSIPEIAYQVRLKEEVAV
                     ICSMGFAGYFLIVWDIIKFARSRGIPVGPGRGSAAGSLVAYALRITDLDPLVYSLLFE
                     RFLNPERVSLPDIDMDFCMDRRDEVINYVIHKYGEDHVAQIITFGTMKAKAAIRDVGR
                     VLEMPYAEVDRIAKLVPDDLKMTLDKALEQEPRLKELVDKDVKVKELMSVAQSLEGLA
                     RHASTHAAGIVISDQPLTEHVPLYKGPKDEIVTQYSMGDVEKIGLVKFDFLGLKTLTM
                     IHRAETLVNEVRPGQPPLLVEQLAFDDPETFALLSSGKTTGVFQLESSGMRDLLMGLK
                     PDRFEDIIAIIALYRPGPMDLIPDFIKRKQGKIPIAYEMPELEPILKDTYGVIVYQEQ
                     VMAIANKVAGFSLGQADILRRAMGKKKPEEMEKLRVQFLEGAKQKKISEKKADKLYEL
                     IQKFAGYGFNKSHAAAYAVVCYQTAYLKAHYPTEFMAALMTTDMGNADKIVGYFTECR
                     GLGIPVLPPDVNQSQKNFAVVDKSIRFGLAAIKNVGEGAVEAIIEVRNETGPFRSFFD
                     LFRRVDLRKLNKRMMEGLIKAGAFDSMGGRRSQYLAVLDQAMDEGMSIQKERALGQTS
                     IFGDDTIGMPQQLDEPALPDVPEWSQGELLKYERELTGFYISAHPLARYEAAIQLFAN
                     TTTMQIPDVPDGREVKLCGIITAVKSMLTKKGDRMAYITLEDLHGLVEVIAFPDLYRD
                     HADMIVPERVVRLTGTVDRGDKGTKLRGTKIEPLADLQNTGISRVMIRLHDGPTASTR
                     LPMLRQVFQKYPGASSVSLVMALGGTMEARTSPLPNVKITPSEHFVADIEEVLGKGAI
                     TLVT"
     gene            13477..16701
                     /locus_tag="PK224_13095"
     CDS             13477..16701
                     /locus_tag="PK224_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16724.1"
                     /translation="MPGASREAKAPLTQFSGRRIVSMAEQKEEKSKLPLTGIVALLLA
                     VVSSLIIYQVPLKTSRPIDKEAEKAVSIGADRVQARLWQDPFEAVSTHRQKEAAVEKL
                     PDSDAHHTFLKLLTAVTQAGAPSDFLLLPVFVDGSPYASGVESRLRDRYALVSALGAA
                     GYQPESGESIRYFVRPGNGHLVVPVEAFLGRPKPAEPKQSTQVLVLWLKEQDFSPKPL
                     LSLSELVTEVQQAFKQSTVSYRVVGPRSSGSLGAMVGELTALYGADQSSGGSSAASLA
                     ASRRQLDSLKGVQFYSSWATAADPVLLGELQGPDAARGVEQWFQAGGLRLTRTIGTDA
                     VLAEQLVEELKRRRIDLTIPRGEAGPCKEADSDSAAVSSRHSDGKCRIPHIALISEWD
                     TLYGRSLPRTFVAMARSLAETGPDRAMRGFEYHFNRLRSEQYPDWVHRYSYLAGLDGE
                     LPPKNTEKDSGGAQTKGKIGDGGLRVEQSLAETPAGRSQLDYLRRLVETLKREEASND
                     GEFKAIGVLGSDVYDKLMILQALRSDFPHALFFTTDLDARLTHPSQLQWTRNLVIASH
                     FGLELHPLIQTPIPPFRDSYQTALFYSVLRAVNDIVPGPGPGELQIPATVTFPKTVPP
                     RLYEVGRHGPVDISLDGPYDAGRRPEALPNLHLPRPDLDPDTGELRVLRPQTIALFLS
                     ALSLMALCALLINSELWQWAQSRWVQGAVVGLVLMVCLLLGLLCWAMSDRTAGEPFSL
                     TDGISVWPTTVLRLLAFLLCLLFLGHSWRRLRDNETQLSRRFRFFRPRAPLSIQAGSL
                     VGIHHWRPQPRGDAEAALLWREYRALGSWKERLVRIVPQALCYLGFGALLLVISGFPV
                     MPCRGSACFTISYAVLGFSVLAMTLLMFYVVDATRLCRRLIKIMVGTTIWWSDRLLVR
                     EAAKRGVDQAYVHEWIAVEFIAKRTAVISAMIYYPFVVVFLMAVARHSYFDRWDFPLG
                     LMAIFGVNAAYAFGNGVFLRRSAEQAKRAAVAQLKSRLDGLSGEVVFKKEKREQVERI
                     VALIEDYQEGAFLPFTRHPLFGAIALPTGGTGLVFLLEYLATIF"
     gene            16813..17754
                     /locus_tag="PK224_13100"
     CDS             16813..17754
                     /locus_tag="PK224_13100"
                     /inference="COORDINATES: protein motif:HMM:NF016100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16725.1"
                     /translation="MTKKSTGKRAQVGKSPASPGRNRTGTDAFMADLNRIMREQVFQS
                     LDDAQAFMTRLMEEGGGRLPLPRAQAPAERAQELIYQAWEARTQRQAVALAREALEIF
                     SDCADAYNVLAEAEARSPQEACTLYQQGVDAGRRSLGKAFFDEHRGYFWGMIETRPYM
                     RARRGLADCLWALGRKRESLTHCEALLELNPDDNQGIRHGLLSRYLTLGNDTGAARLF
                     RDYAHDASAAFLWSRVLLDLRRGDQVAAKEHLVLAMDGNPHVAGFFAGKRKPPARLPE
                     HYSPGDRNEAVLYIANFAEAWLASPDAMDWLTDQLAN"
     gene            18037..19029
                     /locus_tag="PK224_13105"
     CDS             18037..19029
                     /locus_tag="PK224_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16726.1"
                     /translation="MFHESCFVLRAFFRHAAGSLYHIVIVAMSAGIALLLPAGAGRFL
                     SFWSHVEHDKFSLIAVEMTAAVLLMGCFNYVHRSLRDRALAEAALGAGLVSFFPRRGP
                     QARRRIQALKDEQGTGRSVMVIGSSGAGTLVDQVGDLSSVLDKCLEAKILLVNPFSQD
                     ARARMQALAHPACTYPAFREEVRQSIALLKRLKAIGKVVKLKLYADAPLVKLVILGDY
                     LWLQHYHADLDIQNMPEYVLQRNRKEHGLYTFYAHYFMQRWESPELPEYDLDTDELVY
                     RSRTGQEIRRESVEIGAAPVAAHSAETARVDTREAFQLLHAPGPYLPARGYDVE"
     gene            19185..20369
                     /locus_tag="PK224_13110"
     CDS             19185..20369
                     /locus_tag="PK224_13110"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRB16727.1"
                     /translation="MGNGVERRAVSPMTTFFSYGFRPFFLGASLFAGVAIPVWILILA
                     GTGNPVLLSASRDWHVHEMLFGFLPAVIAGFLLTAVPNWTDRPAIQGRELMLLFTLWL
                     AGRAAMAIPLVVPIFAAIVDAAFLVALVGLLWRELAAGNSWNHAPVAVAISLYAAANV
                     TFHVLTVRGAETDLALRLALALDLFLLTFIGGRLTPNFTREFLAGQGKKERPAPFSRF
                     DALSIALVLMAALAWTVLPLASATGWIFLAAGTVNLVRLLRWYGWLTWREPLVLSLHV
                     GYGWLALSMLVLGCALLGIGLAKEDAVHALTTGAVGSMTLAVMTRASLGHTGRPKHAG
                     PATVFIYGLVILGAMLRVFGPGTGLPTNLVLGSAGAAWSGAYLLFALVYGPYLVRPSL
                     DE"
     gene            complement(20471..21721)
                     /locus_tag="PK224_13115"
     CDS             complement(20471..21721)
                     /locus_tag="PK224_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16728.1"
                     /translation="MGAMILQMVGCLLVAATIGLMMGWLLRSFATSEKRQQLSEIATR
                     LRGREHELDTLNHELKVRTSAVQMLEGKMITSEAALKDLTADLATKVEQLTTLQTEVR
                     EKGVRLHAAERERDTLRREVEEAEARIKAQSTGFAEMQAQMENAEDVLIARDQEIATL
                     KTWVEQLAPKDAEIARLRTRAEELEPFLEKSRRLEREQEQDRARASAILQTKEQQLAQ
                     TQARLSAIELDLANLRTQSQEDLARFGETLQERDAEIQRLRATVEELDMFRGEVEKKD
                     LALREAEERRVMDVSEREEEIGALRKRLVEYRVAQRFSAQAKSTGATESGSTGSATTK
                     RSAGKGHPAQKDDLKQIHGIGPVMERVLNRMGMFTFRQIAEWKEQDVEHMASELNTFP
                     DRIRHDNWIDGAKEQHFLKYGEEL"
     gene            complement(21726..22631)
                     /locus_tag="PK224_13120"
     CDS             complement(21726..22631)
                     /locus_tag="PK224_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB16729.1"
                     /translation="MSRGIIFGVGLFALTLLAFLCIPRHLPVTSSATGHSAFSAHIEN
                     GQLTLSGTIASEEAKGAAVARAQDLAKSLRLRVTDNLNIIEDGQAAGWEAALPALLTQ
                     AVTLQHHQATLSLSGQTVTVKGAVPSADAKTKLLHEVASLLGTSIHVQDQVTVASSGS
                     ASQTNPAPASQPSAAPHASRAQVQAGLDEILRGEHIAFESNSAVLTSKGRAVVDKLVP
                     ALKRAPEAVIEIGGHTDSYGDPDYNLQLSRTRAEAVRQYLVEHQVPNRLTAVGYGSTR
                     PLSQDRTRAASKKNRRIEFRVKEER"
     gene            22984..24537
                     /gene="purH"
                     /locus_tag="PK224_13125"
     CDS             22984..24537
                     /gene="purH"
                     /locus_tag="PK224_13125"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRB16730.1"
                     /translation="MASIARALISVSDKTGVVEMAKGLAALGAEVLSTGGTAKALREA
                     GVAVTDVAAYTGSPEILDGRVKTLHPKIHGGLLGRRRVPDHVAQMQQHGIGNIDVVVV
                     NLYPFESTIAKPNCPFEEAIENIDIGGPSMLRSAAKNHEDVLVLVDPADYGRVLEALK
                     SGSVTPALRRELAMKVFQHTARYDSLIAGYLEKQVQGSEVKFPAILSLQFERVETLRY
                     GENPHQQGAFYREMNAKEPAVSRGKILHGKAMSYNNFLDSNSALELVKEFEQTAVAII
                     KHNNPCGCALGATPVDAYVKARATDPVSAFGGVIAFNRPVDLAAAKEITSTFVEVVIA
                     PGFAEDALAELKRKKDIRLLDVGPLSKATAEGYDLKKLVGGLIVQDRDLGTIKDIKAL
                     AVPTSRKPTEEEYAACAFAWVVCKHVKSNAIIYGRPGEIVGIGAGQMSRVDSVKLAVM
                     KAQSPVKGCVMASDAFFPFRDGIDAAAEAGITCVIQPGGSIRDPEVTKAVDEHGMAMI
                     LTGMRHFRH"
     gene            24783..26060
                     /gene="purD"
                     /locus_tag="PK224_13130"
     CDS             24783..26060
                     /gene="purD"
                     /locus_tag="PK224_13130"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRB16731.1"
                     /translation="MKILVVGGGGREHAMVWKIAQSPRKPQIFCAPGNAGIEGLATCV
                     PIKADDIEGLKAFALKEQIDLTVVGPEAPLALGIADEFRKARLKIFGPTKAAARLESS
                     KSFSKDIMAANRIPTAAARSFEHMDQALAYLDEQPVPIVVKADGLAQGKGVVVATTRE
                     EAKQAVRDAMEKSLFGQAGHRVLIEEFLDGEELTLMAFTDGKTVVPMVPAQDHKRVGD
                     GDSGPNTGGMGAYAPAPIATAALREQVTRQVLQPTVDALARLGCPFQGVLYAGLMVVK
                     GTPYVLEFNARMGDPETEVVLPLLKTDLVEVMEAVVEHRLDELTVEWHPDTAVCVVMT
                     SPGYPGSYPTGLPIQGLPTPFGESRVAVFHAGTKRESGRVVTAGGRVLAVTAWGPSLL
                     DARAQVYQAVPSILFEGRHYRTDIAHRALPHKI"
     gene            26087..26734
                     /locus_tag="PK224_13135"
     CDS             26087..26734
                     /locus_tag="PK224_13135"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRB16732.1"
                     /translation="MALILPFTDTTCDRVLPDARQVLDAHGVLALPTETYYGLAVRPT
                     DETALRRLIELKGRPSDKPILVLIGSLDQLPQLVQAIPPAAALLMEQFWPGPLTIVFP
                     ASPDLPALLTAGTGTIGVRQAPLLQLQHLLRHTGPLTGTSANRSSEPPLDNAVAVQRA
                     LGAGVDLILDGGHTPGGLPSTMVDARDRPHLLRAGALSTDLIRAALARQGYELSS"
     gene            26783..27226
                     /locus_tag="PK224_13140"
     CDS             26783..27226
                     /locus_tag="PK224_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16733.1"
                     /translation="MLIRSRRAVMGLSTLGVGAMLLLSGCDFWPPALQAQIEQLHSEA
                     QQAAADKALLQNQLNAANKAKEELQIRVEDLTRVNKEKSAMIANLEHTIAAAKERAVK
                     ATKSSAVKPVAKSAVKKSVKPTHKPAAKSSVRSHSTKKTKPAPVR"
     gene            27549..27782
                     /locus_tag="PK224_13145"
     CDS             27549..27782
                     /locus_tag="PK224_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16734.1"
                     /translation="MLEDDEELEPDVLVPDVDVLVLVDVVELGAAAGAGAGVVVELVP
                     PPSEEAGAFVLSALDAAGFSEVSLPEPGFILSE"
     gene            complement(28084..29277)
                     /locus_tag="PK224_13150"
     CDS             complement(28084..29277)
                     /locus_tag="PK224_13150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRB16735.1"
                     /translation="MKLAARVGRIVPSPTLSITATAKSMAAQGIDVIDFASGEPDFDT
                     PEPVKAAAEAAIRAGFTKYTPSSGIDELRVAIADKLKEEQGLQYDKSQILVSCGAKHS
                     LYNLAEALLEAGDELIIPVPFWVSYQDQTLLNDATPVLLQTREEDGYTISQEALEAVI
                     TPRTKAIIVNSPCNPTGATYDRTTLEGIAAAALRHDLVIISDEIYEKVLYDGTQHISI
                     ATLSPEVAARTVVINGVSKAYAMTGWRIGYAAGPKPLLTAMANIQSQSTSNPCSISQK
                     AAVAALRLGNPFTKTMVTEFDRRRRVMVDRLNKMPGVTCRMPTGAFYAFPNVSGLLGK
                     RWKDQPIGSAANLATYLLNEAQVAVVPGEPFGSDIHIRLSYATAMEAIERGLTRIDAA
                     LRRLS"
     gene            complement(29274..29762)
                     /gene="coaD"
                     /locus_tag="PK224_13155"
     CDS             complement(29274..29762)
                     /gene="coaD"
                     /locus_tag="PK224_13155"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRB16736.1"
                     /translation="MKTAVYPGTFDPITHGHSDIIRRGFRMFEKVIVAIAPNPGKHPF
                     FSVKERLEMVGLVTKDLPNLEVTTFEGLLVDFVRECGAHAILRGLRAISDFEHEFQMA
                     LINRKLAETVETVFLMPSEEYSYLSSTIIKDVASHGGSLQDFVHPEVARRLQERIRSF
                     KG"
     gene            complement(29759..30331)
                     /gene="rsmD"
                     /locus_tag="PK224_13160"
     CDS             complement(29759..30331)
                     /gene="rsmD"
                     /locus_tag="PK224_13160"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRB16737.1"
                     /translation="MRVIAGLHRGRRLLGPRGQAIRPTSDRVKEALFSILGERTTGAR
                     VLDLYAGTGSIGIEALSRGAAHATFVEADRQALRLVNSNLEQCGLQQSANVCACQVSQ
                     FFRRATQWSGPYDIVFCDPPYQLTPELIAMAQEWNAGWLADDAVVIIEHGKKAEIPQT
                     LGLLSQVKRYDYGDTALTRFHVTAKEAQPR"
     gene            complement(30642..30815)
                     /locus_tag="PK224_13165"
     CDS             complement(30642..30815)
                     /locus_tag="PK224_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16738.1"
                     /translation="MECPKCKGMMMLERFSDFFLIFYAWKCINCGAIIDRTISANRRK
                     SLAAREAQPVATR"
     gene            31001..31717
                     /gene="radC"
                     /locus_tag="PK224_13170"
     CDS             31001..31717
                     /gene="radC"
                     /locus_tag="PK224_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRB16739.1"
                     /translation="MTPKKTGRGIGKWPETERPRERLLREGAESLSDAQLLAILLRVG
                     RQEASAVKVGMEVLDRVGGIFGLLHCSAEELCAIPGVGPAKAAQLKAAVEVGKRAVSA
                     PLTTGTRISSSADLFKHYHARLRDLRHEIFAVVLLDAKNQVIRDVTISEGSLTLSIVH
                     PREVFIPAMRASAAGVIFLHNHPSGDPTPSQEDRVLTARLVSAGSLLGIQVLDHLIVG
                     DGRYVSFADQGWLNGEGARV"
     gene            31851..33956
                     /gene="fusA"
                     /locus_tag="PK224_13175"
     CDS             31851..33956
                     /gene="fusA"
                     /locus_tag="PK224_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRB16740.1"
                     /translation="MNVPLTESIRNVAVVSHAGSGKTSLVEALAYCAGSLSAPGSIFA
                     GTTVSDFEPEEIHRRTSLNTTVLRCSYQDRVLTLLDTPGSPSFIGETRSALRAADGVV
                     LVVSAASGVRSALQRIWSMVRDSGLPCVVFVNDLDKDGTVFDNTVEDLTKELEMTAVP
                     LILPMGGGAQLGDVVDVLQNHVVTSQPDRPKSQQGPVPPEWRDRVDQARRRVMELVAE
                     GDEALLERYLTDGALTEEALLEGLQAGVQRGSLVPIVGGSALRHIGVHSLLRTLVELL
                     PSPVSRARVAPLQGTGSTEDGGPVNRHPLPTDPFSAVVFKTLIDPFIGRLSYVRVYSG
                     TLEADTAMYNSTRQVRERGGHLFSIFGKKYTPITRATAGEIVAIGKLKDTVTGDTLCD
                     EHAPIRYPGIPFPRPVVSFAIEPKSKTDIEKVSLGLHKVIEEDPSLEFVRHIETKEMV
                     LSGMGQLHIDVALEKLHRKYGADVIVHAPKIPYRETIRSVAQAQGKYKKQTGGHGQYG
                     DCWLEVGPLPRGQGFEFENKIVGGAIPRNFVPAVEKGVVEALHEGPLAGYPAVDVRVT
                     VYDGSYHVVDSSELAFKIAGSMGVKKALEAAHPILLEPLMTVEVEVPAECVGTVLGDL
                     NARRGRIVMVEANGHIEIVKALVPQAEMLNYAASLNSMTGGQGSYAMEYAQYEEVPRE
                     LAGRIIEEHKMERHGAVAH"
     gene            complement(34000..35406)
                     /locus_tag="PK224_13180"
     CDS             complement(34000..35406)
                     /locus_tag="PK224_13180"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRB16741.1"
                     /translation="MKTQTSFATALILALGFSGWLTGTSAITLAATDIASPPRKESPG
                     LRMLEELQTVITDLAEEAKPSVVSIFPIQTLGKSRDGSGERVPNSTGSGSGVIVDPNG
                     HIITNNHVVGDATEVEVRLSDKTKLFAQVVGKDPDTDLAVLKVTTDHPLPAARFGDST
                     GVKVGQWVLAVGNPFGLDRTVTLGVVSGIGRENINLSRYENFIQTDASINPGNSGGPL
                     FNLRGDVIGINTAIINFAQGIGFAIPSNMAKQVMNQLISKGKVVRAWLGVGLQPLTPD
                     LANKFGVDENEGVLVNEVFERDPAALAGIKPGDVITKVDGALVDTPNKLSRLVAALDP
                     GSTTHVEVVRDGKRLTLNVALSERRDAVVTASLPQSRTDLKLGIDVQDLTAGLAEKFR
                     LNESRGVLISKVEAGSLAQAEGLREGDLVKEVNRVDTGTVGEFTVAVSKVKRGDTILL
                     RVLREGRAFYVVLKPADR"
     gene            complement(35494..36132)
                     /locus_tag="PK224_13185"
     CDS             complement(35494..36132)
                     /locus_tag="PK224_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRB16742.1"
                     /translation="MLSFELLARVRKELFLTGSALYETIVAIAERVNRKVHVLRLHGQ
                     ATTILNQIHAIHRQVGRRIAELGTKPSGAIPSVAPGTLPSLENAIRTASDRIKGQQTA
                     LQQIERHIRDLKIELAHEDLLTMQRELGLRDAAIERVLVARGAPVIGHPIGEFDLPST
                     THIVAVFRGPFLLPLSDSMTLRPDDIVILVGLRHDLAHWLPHFQRPAASKSA"
     gene            complement(36162..36764)
                     /gene="plsY"
                     /locus_tag="PK224_13190"
     CDS             complement(36162..36764)
                     /gene="plsY"
                     /locus_tag="PK224_13190"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRB16743.1"
                     /translation="MDSPWLIGFLILSSYLLGSIPFGVVVSRLLGAVDPRSAGSRNVG
                     FTNVLRVSGKKAGILTLLGDIGKGWLAGWVGTMLLHQESAILWVALASVIGHLHSAFL
                     GFKGGKGVATALGAALGVAPWIGLTLMGLWVGAVFLWKYSSGGALFAFAMFPIVALLF
                     HRSWTFIGFACVVSLLIWVRHKDNLVRLWSGTESRIGQAG"
     gene            complement(36813..37427)
                     /gene="pgsA"
                     /locus_tag="PK224_13195"
     CDS             complement(36813..37427)
                     /gene="pgsA"
                     /locus_tag="PK224_13195"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRB16744.1"
                     /translation="MAENWKQAGLVLMRSAGQESNINLPNVLTLVRILLIPVFVMLLI
                     DPTPDRALAAAIVFVVAAVTDLLDGYVARKTGQITKLGRLLDPIADKLLVLSALILLV
                     QVDRVSALVAILIIAREVAVTGLRAIAASEGLIMSAEVTGKYKMALQVIAIVLLVLEG
                     TVVEVIGNLHLAGIVTLYLSLILGYVSGAQYVWSFWRQVGAKGL"
     gene            complement(37473..38426)
                     /locus_tag="PK224_13200"
     CDS             complement(37473..38426)
                     /locus_tag="PK224_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRB16745.1"
                     /translation="MQEGRRVLEIEARAVQELMARLDDRFASAVNFLYECQGKVVISG
                     MGKSGLIGQKIAATLASTGTPSFFLHPAEGVHGDLGMLARRDVLIAISNSGETQEVLQ
                     LLPFVKRMNIPVVGMTGKMASTLAKNSDVTLDVSVDEEACPLGLAPTASTTATLAMGD
                     ALAVALLQKRGFKQDDFAQFHPGGTLGRRLLVKVRDLMQHGDHLPRVRDTVSGADTIL
                     EMTSKKLGMTTVVNAKGALYGIVTDGDLRRFIQAGGDFSKVTAGDLASRQPKTIGPDE
                     LATTAVALMERFSITALVVLERPHRLAGVIHLHDLLKHGIV"
     gene            complement(38489..39310)
                     /gene="kdsA"
                     /locus_tag="PK224_13205"
     CDS             complement(38489..39310)
                     /gene="kdsA"
                     /locus_tag="PK224_13205"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRB16746.1"
                     /translation="MAYEVDLGQFKIGAGNRPFLIAGPCVIENEQLVIDTAGRIADIA
                     KAIGMPYIFKSSFDKANRTSITSFRGPGLEKGLAVLARVKQHIGVPVLTDVHSEEQAT
                     EAGRVVDILQIPAFLCRQTDLLIAAAKTGKVVNIKKGQFLSPPEMGNAVKKVEDSGNR
                     RIVLTERGSSFGYNNLVVDMRSFPIMRRFGYPVVFDATHSVQLPGGGGTKSSGQREFV
                     EPLACAAAGAGCDGFFMEVHPDPDSALSDGPNMVPLHTLQSLLERVLRIWDAAAK"
     gene            complement(39618..41219)
                     /locus_tag="PK224_13210"
     CDS             complement(39618..41219)
                     /locus_tag="PK224_13210"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRB16747.1"
                     /translation="MSKLIFVTGGVVSSLGKGLASASIGNLLESRGLKITFLKLDPYI
                     NVDPGTMNPYQHGEVYVTEDGAETDLDLGHYERYTSLTLTRENNYTTGRIYHAVITKE
                     RRGDYLGGTVQVVPHVTDEIKQCIMRISQGMDVTIVEIGGTVGDIESLPFLEAIRQIP
                     YDVGRDNVLYVHLTLVPYIGAAGELKTKPTQHSVNKLREIGIQPNILLCRTDRYLPPE
                     LKAKIAMFCNVEKDAVITAKDVETIYEVPIVFRKEGLDELIVRLLKLETGPPNLREWD
                     AMVQKIKHPKHEVSIALVGKYAGLKECYKSLAEALVHGGIDHETRVNVNWIESEEVER
                     QGTERILREADGILIPGGFGARGIEGKIVTIQYAREHQIPFLGLCLGMQCATIEFARN
                     VAGLVGANSAEFDEKTPHPVIHLMSDQQSVNDKGGTMRLGAYACKLGEGTLAQKMYGV
                     SEVRERHRHRYEFNNAYREQLTAKGLILSGLSPDGRLVEIVELQNHPWFLATQFHPEY
                     KSRPHHPHPLFSGFVGAALRRKCGH"
     gene            complement(41237..42007)
                     /gene="kdsB"
                     /locus_tag="PK224_13215"
     CDS             complement(41237..42007)
                     /gene="kdsB"
                     /locus_tag="PK224_13215"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRB16748.1"
                     /translation="MKRSVTVVIPARYGSSRFPGKPLVELLGKPMIQHVYEQAQACRA
                     VDDVLVATDDERIKTAVQRFGGQVVMMTEPYRTGTDRVAAVADSRTGDCFVDLQGDEI
                     LLHPDLITDLVEPFLASEATMGTLKRQIDSDQDLHNPGVVKVTTDREGYALYFSRAPI
                     PLVRDDPKRAAVPGLHFIHLGLYIYTRETLQRLTALPTGTLEEAEKLEQLRALENGMR
                     IRVWETTHGSLRIDSPEDVPGALKQLHSRATGLGITHS"
     gene            complement(42009..>42301)
                     /locus_tag="PK224_13220"
     CDS             complement(42009..>42301)
                     /locus_tag="PK224_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRB16749.1"
                     /translation="PRLSKKSPAVIIRARRGMSVYQAHGDHWHIPTRARQVYDVTGAG
                     DTVVGTLALALSTGASMRDAAVLANQAAGVVVGMIGTATVTAAQLTDALGQN"
BASE COUNT         7833 a        12070 c        13355 g         9043 t
ORIGIN      
        1 ggatcttctc tccacgatga tgagcccgat cggttcgttc ggtgacaagc tcaaggtgct
       61 ccggctccgt cgggatgcgt tgcgccggca gctctgtggg caggcgggcg gaggcgagcg
      121 caccacgctg gaggccctgc aggtctatgg gttttctccc gccatgcagc agcgcttttt
      181 ccagccgttt ctgaatggcg tgtttctgga tcaggcgttg tccacgccct gccggctgtt
      241 tgaattggtc tgggcggcgt tctctcgcgg cgatattgcg ctgccgcaac agggcatggg
      301 cgcgattgcg agccagctgg ccgcgactct tccaacggag tcgatccgat tgaacgcagc
      361 ggtcagtcac ttggacggca ccaccgcggt gctggcgtcc gctgaacgac tcgccgccga
      421 tgttctggtc ctggccacgg atgatgccac cgctgccacg ttacgaggcg atagccgggc
      481 ggccgcgccc gggcgtgacg cgatctgtct gtatttcgac gcgcccgctc caccggtccg
      541 tggaccctgg ttgattctga acggagacgg tcagggaccg gtaggcaccg cctgtgcctt
      601 gagcgaggtc gcctccggtt atgcgccctt gggacggtcg ctgatttcag tcagcttgtc
      661 ggggcaacca tctctcgccg gcggtgatct ccagcaagcg gtgcgccggc agttgcgcga
      721 gtggtttggt gaggaggtgc atgtctggcg acacctccgg accgatcaca tcaagagagc
      781 gctgccgccg attgaggtcc tcacggcgca ggcatcaggc gcatcaccgc gcgtgaggcc
      841 ggggttgtat caatgcggtg attattgcga gaccgggacg ttggatgggg cgctgctgtc
      901 cgggcgaaaa gccgccgagg cgttgttggc tgaccattga gtagagtcgc agacgggggg
      961 cggtcttgaa cggacgggga gcgcgggtca cgaatcattt cggatggcga tggctgttgg
     1021 cggcgacgat ggtgctgttg tcctgtccgc tgccgtcggc cttggccgat ccgtcctccc
     1081 agcatcagct gcaggcgctt gccgggcagg gcgatgcgaa cgcgcaatgg atgttgggac
     1141 aggccttgct cacgggcagt ctcggggaaa ccgatgaggc cgaagcggtg aggtggctgc
     1201 agttggcggc cgaccagggc cacgccttgg cgcaacgcga catgggcatg ttgtatgagc
     1261 aggggcaggg ggtgacgcag gatgtgctgg aggcgttctt ctggtattcc cttgcgagtc
     1321 gtcaggacag cagtcgggcc agactccgcc gtgatgcgtt ggcggcgatg ctcaccgcgg
     1381 aacaacatga ggccattgcg gcacgtctca agggatggcg gccgagaaaa taattggtcg
     1441 ttgtcggggg ttattttttt cgtctgaaga gatcggtact gagtgactga atacggtcga
     1501 ggaagagcat gccgttcaga tggtccactt catgctgaaa ggcgagcgcc tcgaatcctg
     1561 acgcactcac cgtgacgacc cggccgtccg gcgccatccc ctccacgagc gcctgttcgt
     1621 agcgcagcac atgacccgta tagtccggca cgctcaggca gccttcccgc acgctttttt
     1681 tcccttccag cgagagaatg accggattca acagcacgac caatccatgg cccccctcgc
     1741 ctttcttgcg cgacacatcc acgacgatca cctgcacggc atgcccgatc tggggcgccg
     1801 ccagtgccac gccgggtgac gcggcgagcg tatccagcat atcttgaacc accgcttgca
     1861 cggccggatc ggagggggcg acggcggcgt tcgtggattt gagtgcctga tggggatact
     1921 gcagaatcgg acggatagcc acgatcagaa ggtgacactt tcgagcaggc ggaacgtcag
     1981 atcgacgccc agcagcggct tgagttgttc aagagcctgt ctgagctggt cggcttgctg
     2041 agcctccgac aattgaatct ccaggaccat gacatagatg ggacgctctc ccgatccaac
     2101 cacgcgggta ttcatgtcgg tgatgttgcc gttgtgctgg gcgacggtgc gggccacctg
     2161 tgccacgatg cccggatgat cggccccgta aaccgacaac atgaacgtcg gtacctccag
     2221 tgccgtctgc ctggttgctg cctgatccgg catcgctctg cacaagacgg caaggtccag
     2281 cggagtcgta tagggcgtca ggcgtcggcc aagctcctcg gcggcgattc cctctggaag
     2341 gcgcaccatg agcatcatgg tgaactcgcc acgaagccgg gtcatgcagg tgtcttcgat
     2401 attacagccg agtcggtaga ggctatccgc catcagggcg acgataccgg gacggtcttg
     2461 tccgaaggcg gtgacaatcg cgaaatggtt cattggcatc tcgtgggtcg caagagtggg
     2521 tgcaggctgc gagtagaatg ctgcaattgt ggaagattag caagcgccgg tcgcacgtgc
     2581 gtggttggac aagcggaagg gcccggtcct acgttcgtcg ttttgtccga ttcgtgggtt
     2641 gagcggtgag tggatcatcc ggccagtggt ggcgtgggta gcggcctcgc agttctttct
     2701 tcacctcttg ataagtcgag cgccaaaagc ttgccaggtc tttcgtgacc tggacaggcc
     2761 gtcccgccgg cgagagcaga tgcaccatca ccggcaccct tccacccgcg atgcgcggtg
     2821 tctcctgaca gccgaacatt tcctgtaagc gcacggccag caccggcgtc tcgctctgat
     2881 cgtagtccag tcgcacatgg gagccactcg gcacggtgag gtgtgtgggc acgagcctgg
     2941 gaagttcctg ccgttgctgc catgtgagca ggctttcgag cggcggttgg agatcgatgc
     3001 gacgaacctg cgcgaggctt gtgaggccgc tgacaaatgg ccccagccat gcgtcgagct
     3061 tgtttatgag cgcgccgtct gacaggtccg gccaagtggg gtcgatgcga tgtagaaacc
     3121 cgacgcgtgc gcgccactgt tgcagctctt tggcccaggg gagacaggtg agtccctccc
     3181 gccggatgcc gaagagcaac gctgcggtga cttgaacagg gtccgggtcg tgggtcgcgc
     3241 gatcgtcgag gatgagttgg cccaaccggc gctgacgtct ggcgcgcacg gattccgacc
     3301 ggtcatccca ttccaacagg tcgacggctt ggatctgctc ggcgcaatgg tgctgcagat
     3361 ctaccaatcc aaccggcgcc gccaggagga tacgcgccca gtcgccggtg ccgtcgagtt
     3421 gagccaccac cagatactct tcttgagaga gaccttgtac ggtgtgaaat gccgcgcctc
     3481 ggccgttggc cagccgataa cgaccctcgc tgcctgcgat tcgctgcgcg atgcggtcgg
     3541 gataggcgag ggcgagtagt gtgccggtat ggtcggtgcc gtcgacggaa gatggtgtga
     3601 gctgcagttg gcgtcgccac tgttcggagg cgcgttgcac ccgttcacaa ccggcgcggt
     3661 tgatggtcgc tcctgcgaga tggtctctgg tgccgcgcaa cgcttcaact cggatgcgca
     3721 ggtcggcgtt tcgccaaccc ggtccgccct gcagaatgtc ccgttcactc agcagtgccg
     3781 ccaggtcgca ggcgtgggca ccgaggccga tcggcacagc ggtgaccatc atgtgggcca
     3841 gtcgtggatg aacagtgacg tgggcgagcc ggcggccgtg cgcggtgagg gctccttgcg
     3901 catcgagcgc tccgagctgt cgcagcagat cacgagcctg ggccagcgtg cctgtcggcg
     3961 gcggatcgag gaaggagagt tcggccgttt caatgacgcc ccattccgcg agatccagtg
     4021 ccagcggagc caggtccgcc tccaggattt ccggagggcg gcgagggagg agcgcttgct
     4081 gctcggccgt cgtccagagg cgataacagg tgcccgcttc caatcggccg gctcgaccac
     4141 gtcgttgatc ggctgcatcc tgggtcacac gaatggtatc gagccgcgtc agccctgagc
     4201 ggggatcgaa gcgcggaacc cgcatcagtc ctgcatcgat gacgacccgc acaccctcga
     4261 tggtcaaact ggtttctgcg attgaggtcg cgagtacgat ctttcgtcgc cctgtcggcg
     4321 cggggagaat cgcctgttcc tgttcactct gcgggagttc accatgcagc ggtgcgatga
     4381 gaatattcgg gccgagggag gcctcgacaa gttgccgctc gacgcgccgg atctccgcca
     4441 tgccgggaag aaacaccaac agactgccct cgtcacgagc cagggcctga cgaatgctcc
     4501 ggaccactgc cggttccaga tgcccttcga tcggccggtc gagatattgc gtggtgacgg
     4561 ggaacaggcg gccttcgcag gagatggtgt ccgcatcctg cagcaggcgg gtgaccgccg
     4621 cgcagtcgag ggtcgccgac atcacaagca ggcgaaggtc cggtctgaag aggcgttgcg
     4681 attccctggc gaaggccaag ccaagatccg cctgcaggct gcgttcgtga aattcgtcga
     4741 agacgacgag gccgtatccg gcgagtgacg ggtcgtgctg cagcagcctt gtgagaatgc
     4801 cttcggtgac gacttcgatc cgcgtgtcct tcccgaccct ggtatcgtgg cggatgcggt
     4861 acccgaccgt cttgccgacg ggctcaccta tcattgcagc catgtaggct gcggcggcgc
     4921 gggcggccag tcgcctgggt tccagcaaca ccagtttctg tccggccagc cagggttcat
     4981 gcagcagggc cagcgggaca cgcgtggttt ttcccgcgcc cggttgcgcc gtcagcagtg
     5041 ccgcgcatcc ggttgccagg gtccggcgca gcgccgggat cgcctcttct attggtaatc
     5101 ggaccatggt agggtgcgcg ttcaagacat tacttcaatt aatgaagagc gatggcgaac
     5161 tcctgcaatc tatcccaaag gtgaatatgc aaaagggggc ttccgcaatt gttgcttaca
     5221 ccagcccatt tcgattgata ttccgtgatg ccaaagactc atgggcccct tctctcgaac
     5281 agatcaatca gaacggctac gactccataa aattgcacag gctttcaaca tcaattgaca
     5341 ttggattgcc gcaaccattc tgttgccacg tgaccttcga cggagcactg cttttgccaa
     5401 gagttaagca gttttggccc atagaaaaag cagttgccgc cttcaataga gtattaggcg
     5461 aattcatgct tggaggtgtc tatttcgaat cgattgggcc gaccgacatc gatcaagcga
     5521 ttctgtatcc aacgggttac tatcggccac tagggcaagc aaagggtttg ccaggtcagc
     5581 tccgttatgg tttacaagcc aagatggcta gcccgttaca ttcaattctg ctctaccaac
     5641 ctgagcatct tttagcgaaa gacctgcacg ttgcccggaa aaggggcgca gcactctcgt
     5701 caaaatttga aacgctttcc ctagaattcc tggttcaagg aatttcggct ttcgccactc
     5761 acgactgggc aggtgcttta tcgcatcttt ggatttctct tgagcaagtc gtgtcctgcc
     5821 tttggaagga ccaagtaata gttggtggcg tacagcccaa atttcccatt cggaatagga
     5881 aggaattttt gcaggatcac aggacttggg tcacatccgc cagacttgaa gtcctctttc
     5941 agcgtggcat tctttccgag atagcgtatc gcaaactaaa caatgccaga aaagcacgaa
     6001 atgaacttgc acatttgggg agggcacctt caagggaatc cgcttccgct gccttggaag
     6061 gtctattcga agtaatcgag tcttccgaca aatctacctc attggccgag atcattaaca
     6121 ccatcaaatc acgtgatccc ctagaaagat actacagtat gtctggaatg ggagaaagtg
     6181 gtgaaggtgg actttggctg ggaccgcttc ctccaattcc cggggagcca gagtggggag
     6241 tgaaagaata cgaacgtgta tatgataaga agaattagga agcaaatgtc ttatttccaa
     6301 tggcccgatg gggcctcgca ggaagtcgga ctgacgaagg gcgagtgggc cggtccgatc
     6361 gtcccgccga gtgaggattg acggcgcgat gatggttctg cctcagacct gtgaaagggc
     6421 cttgctcgaa cggtttttgc gcgccgaagc catggcgctc tgggcggtgc gggccgctca
     6481 gacgcagaat cttccccgcc acgtccagac gtttctccag cgccacgaga ccgacgagca
     6541 ggatcacctg cggcaattcg aagggatgct gggtaccact tcgcagcggg cgccgactct
     6601 gccgaccgtg ccctcgcagt gggaaatgct ggcggtgttg ttgttcgggt atgaggcgct
     6661 cggactggaa ttcgcgacgc tcctcgcgac ggtgcggccg gacctggcgg acatcctcga
     6721 agacgaacag gtgcatgtcg gattcttcga gaaggaattg agggcgattc tggcgggcgg
     6781 ggagagcgga gctcaacagg cgcgggaggc ggcacgcacc tggtggaaga agctgcctcg
     6841 cacggtggac cggtatctcg gtgatccctc cctcgcgccg taccggactg aacttcggca
     6901 ccacatcctg tcgatcattc aggagcgatt cattgcgttg ggcctgctgc ccgccgggca
     6961 ggccggccgg tagtttccga cccgcctgcc ccgtgctacc tcgtgctaaa actgcagcgt
     7021 gccgttgatg gcggccaaga cctgattctt gttcaggccg tcgttgtatg gtgagcgctg
     7081 cccgtcgaac caatcggcgc gcacttccgg acggatcaac acattggggt gcggccggta
     7141 gttgaggccg gcgctgagcg aatagaagga tccggcgaac ggcccgaggt tcgggttgtc
     7201 gcgcaccggg ttgccgccga ccctggtgcc ggcttcgtcg cgcatccact cgaagcgcag
     7261 accggcccgc cattgatcgg tgagcgcata gtacagatag ttgtcgatgc cgtaccaacg
     7321 cgcggtgccg ccgtcgacct tgccctggtt ttgataggca tagtgatggt ggaagacgta
     7381 ttcccatcgc tcggccggat gtgccgtgaa gatcaggctg tatcgcgtgc gggtttcaaa
     7441 ttgtgtggcc acggcgctgg gttcgttgcc cgtgaccatg gagaatgaga gcgaccagag
     7501 attctccggc gcggtgtact tgatgctggc cagacccgtg gtgctgtcct tcgtccggtc
     7561 gagcgaatcc cagccgttca ccaggccgcc ttgcaacgtg atgcggtcgt tcaggtgcca
     7621 ggtggccagt ccgccccagt gggtgaaggg gccggcgaat tggtaggcaa aggatttgga
     7681 gtagaaaaag ttgttgatgg acggcacacc ttcgtaaccg atgatcgtat agaagtggcc
     7741 gaccttcacc gagaaggtct ggttcccgat ttcggcatag gcctgcggca tcgccagccc
     7801 gtagtgctga ccctgggcgt tccagcgctg cgcgccgttc tcacgccgct cgactccgtt
     7861 gctctgtgtc aggaagtagt cgtagccata catgaaatcc aagcgtccgc cgatgcccca
     7921 attgctgccc gtggcggtca gcggtttctc gacaatcatg tacaactggt tgaggaccgg
     7981 aatgtcgcgg tcgatggcgt tgtagggtcc gctgaagttg ctgcgcgggt tgcttgcatt
     8041 atacgtatac ccgccgtcca cccagccgcg cacgcggagg aatccgtctt gtggaaaaaa
     8101 gcggttgatg cccttctcat ccgcatgcca gagggcgtca gtccacaatg gatccgacga
     8161 ggagcgacct tccaggcccg gcgtttgtgc cgagaagacc ggggtgggcg cttcacccag
     8221 cggggtgccg gtgagcagcg attgcccgaa ggcgccgccc gtccagactc cccaactcca
     8281 taacatggtt gccacggatg cgttcacgat cgacttcgac atacccgtac ctacctcctg
     8341 taaaaaaatc cctgtcacgt gatgaatggc gatcctgcga acgaggatga aaacggggcg
     8401 acgcgtatcg aggtattctt gtcgagacgg gacccattca gcgcacgcag gcgggcggca
     8461 gtgtaacaag tcgtcgggca atgaaaaatc aggacgagta tgtaggtggt ttcagcgggt
     8521 cttgatggcc agggccggat gcaggggata gagcaacgtt aagtcaggcg tgcaatggtg
     8581 gagaggaaga attccagggg gttaggctcc taccggtacg agcccgccga ccaccccgat
     8641 cttgcggaat ttctgatcgc gctgggcgat gagctggtcg gtcggcagtt caaccagggc
     8701 atacaactga ttggccaagg tcttggcgac ccgatcgcac atggcccggg ggtcacgatg
     8761 cgcgccgccc aacggttccg gaatcacttc atcgacgatg ccgagtccga ccagatcctg
     8821 cgcggtcatt ttcagcgagg ctgcggcatc cggaaccttt gacgggtcat cgtacagaat
     8881 cgccgcgcag ccttcagggg aaatgacgga gtagacggaa tgttccagca tgaggatgcg
     8941 gtcgctcacg cccaacgcca gggcgccgcc gctgccgcct tcgccgatga cgacggatat
     9001 gatgggcact gacagtcggg acatgacgaa caggtttctg gcgatggctt cggcctgtcc
     9061 gcgttcttcc gcgccgatac cgggataggc gccgggggtg tcgatcagcg taatgatcgg
     9121 acggccgaac ttttccgcca gccgcatgag ccgcaatgcc ttccggtagc cttccggatt
     9181 gggcatgccg aagttccgct gcatacgctc tttgagcgtc tttccttttt ggtgcccgat
     9241 aatcatgact gagcgatcgt tgaaacgggc aaagccgccc acgattgcgc ggtcgtcgcc
     9301 gaagctgcgg tcgccgtgca gttcgaggaa ttcgcgcgag agttcgttga tatagtcgag
     9361 ggtcgtgggc cgctgaggat ggcgggccag ttgggtgcgc tgccagggcg tcagggttgt
     9421 atagagctgg tgctcgacct gggcgagttt tgtccggagc ttgcggattt cgtcctgcgt
     9481 gccggacttg ggagactcgg cggaggccag cttctcgatt ttttcttcga gttcgcggat
     9541 cggtttttca aagtcgaggt agtctcgcat actgctgaac ccccttaagg gtgttgccta
     9601 ggttcgccta ggttaccaaa gtgatggcgc ccttgcctag cacttcctca atatcggcga
     9661 caaaatgctc gctgggagtg atcttgacgt tggggagggg agaggttcgc gcttccattg
     9721 taccgccgag ggccatgacc agagagacgc tcgaagcacc gggatatttc tggaacacct
     9781 gtcggagcat cggcagtctg gtggaggccg tcggtccgtc gtgcaggcga atcatcaccc
     9841 gcgaaatccc tgtgttctgc aaatcagcca gcggttcgat cttggtcccg cgcaacttgg
     9901 tgcccttgtc tccgcgatcc acggtgcccg tcaatcgcac gacccgttcc ggcacgatca
     9961 tgtcggcatg gtctcgatac aggtcgggga aggctatcac ttctaccagc ccatgtaaat
    10021 cctctagtgt gatgtaggcc atgcggtcgc cttttttggt gagcatggat ttcaccgccg
    10081 tgatgatacc gcagagtttg acctcgcgtc cgtccggcac atcggggatc tgcatggtcg
    10141 tggtgttggc gaagagttgg atggccgctt cataccgggc cagcggatgg gcgctgatgt
    10201 agaagccagt caactcccgc tcgtatttca gcagctcgcc ctggctccat tctggcacgt
    10261 cgggcaaggc cggctcatcc agttgctgcg gcataccgat ggtgtcgtcg ccaaagatgc
    10321 tggtctggcc gagcgcgcgc tctttctgga tgctcatccc ttcgtccatg gcctggtcga
    10381 gcacggccag gtattgcgag cgccgtccgc ccatggaatc gaaggccccg gctttgatca
    10441 acccttccat catgcgtttg ttcaacttgc gcagatcgac ccgacggaag aggtcgaaga
    10501 aggagcgaaa cggtccggtc tcgttgcgca cttcaatgat ggcttcaacc gcgccttcgc
    10561 cgacattctt gatcgccgcg agcccgaagc ggatgctctt gtccacgacg gcgaaattct
    10621 tctggctctg gttcacgtcc ggtggcagca ccggaatgcc gagcccgcga cattcggtga
    10681 agtagccgac gatcttgtcg gcgttgccca tgtcggtggt catcagcgcc gccatgaact
    10741 cggtggggta atgcgccttg agatacgccg tctggtaaca gaccaccgca taggcggcgg
    10801 cgtgcgattt gttgaagccg tatccggcaa acttctggat gagttcgtag agtttgtcgg
    10861 ccttcttctc ggatattttt ttctgtttcg cgccttccag gaattggacg cggagcttct
    10921 ccatctcctc gggcttcttc ttgcccatgg cgcgacggag aatatccgct tgtcccagcg
    10981 agaatccggc caccttgttg gcgatggcca tgacctgttc ctggtacacg atgacgccgt
    11041 acgtgtcctt caggatcggc tcgagctcgg gcatctcata ggcgatcggg atcttgccct
    11101 gtttgcgctt gatgaagtcc ggaatgaggt ccatcgggcc ggggcgatag agcgcgatga
    11161 tggcgatgat gtcttcgaac cggtccggtt tcaggcccat taagaggtcg cgcatgccgg
    11221 aactttcgag ctggaagacg ccggtggttt ttccggacga cagcagcgca aatgtttcgg
    11281 gatcgtcgaa cgccagttgc tcgacgagca agggcggctg accggggcgc acttcgttca
    11341 ccaacgtctc tgcgcggtga atcatggtga gcgttttcag tccaaggaag tcgaacttca
    11401 ccaggccgat tttttcgaca tcacccatcg aatattgggt gacgatttcg tccttggggc
    11461 ccttgtaaag cgggacgtgt tcggtgagcg gctgatcgga gatcacgata ccggccgcgt
    11521 gggtggatgc atggcgggcg aggccttcga gcgactgcgc cacggacatg agctccttca
    11581 ccttcacatc tttatcgacg agttccttca ggcgcggctc ctgttcgagc gccttgtcga
    11641 gggtcatctt taaatcatcc gggacgagct tcgcgattcg atccacttcg gcgtagggca
    11701 tttcgagcac gcggcccacg tctcgaatgg cggccttggc cttcatcgtc ccgaaggtga
    11761 tgatctgcgc gacatggtct tcgccgtatt tatggatgac gtagttgatg acttcatccc
    11821 ggcgatccat gcagaagtcc atgtcgatgt cggggaggga cacacgctca ggattcaaga
    11881 atcgctcgaa gagcagtgaa tagacgagcg gatcgaggtc ggtaatgcgc agggcatagg
    11941 cgacgaggct gcctgcggcg gagccgcgtc ctggcccgac cggaatgccg cgcgatcgcg
    12001 cgaacttgat gatgtcccac acgatgagga agtacccggc gaatcccatc gagcagatca
    12061 ccgcgacttc ttctttcaac cggacctgat aggcgatctc gggaatggag ctcggccgtt
    12121 ccttgagacg cgccgccagc ccttccaggg ccagttgctc cacataggat tcgcgggtgt
    12181 agccttccgg cactttgaac tggggcaggt aggtcttatt gagcgccagg tcgagggtgc
    12241 aggcttcggc gatcttgacg gtgttcgaga cggcggtcgg catctccttg aactcggaca
    12301 gcgcctgctc ggtcgatttc acataaagct gatccgtgtc gaacttcatg cggttgggat
    12361 cgttgatcgt cttgccggtc tgcaagcaca gcatgaggtc gtgcggacgg gagtcttcct
    12421 tcttcaggta gtggcagtcg ttggtgccgg cgagcgggat gccgagcttc ttatggatct
    12481 ccagcaggcc gtcgttggca atccgttggt gctctaaccc gttggcttgc agttcgaggt
    12541 aatagttgtc cttgccgaaa atctcccgat attcgccggc tgccttggtg gcgccctcca
    12601 ggtccttctg tccgatgagg tacgcaacct cgccgctgag acagccagag aggccgatca
    12661 atccttcatg gtgctgctga agaatttcct tatccatccg cggcttatag tagaaacctt
    12721 caagatacgc tttgctcaca agcttgatga ggttctggta tcccttgagg tttgtcgcga
    12781 gcagaatcag gtggtagtag tcgttgtgcg cgaggtgaga attcttctcg agcctactgc
    12841 ccggcgccat gtaggcttcg cacccgatga tgggtttgac ccccgcttcc ttcgccttcc
    12901 ggtagaactc caccgccccg aacatgttgc catgatcggt catcgcgacg gccggttgcc
    12961 cgaacgactt cacctgttgc atcagggggt cgatctggtt ggcgccgtcg aggaggctgt
    13021 attgggtatg gagatgcaga tgaacgaatt gtgatgccac gggcgaattg tagtgagagg
    13081 gaaagttgaa gtcaaacgga aggagggggg agtgcggcct ggtagtctcc tttgttcgcg
    13141 caacgcgcgg cctcagaagg ccctcgttgg acgcgcacag tcggatacca ccaagccacc
    13201 ctccctggat agaagaggag ctggctcgga aggccctcgc ctgggtaaac ggcgcggcgc
    13261 accggagggt tgggtgggta agaaggctgc gcgcagctgg aggctaccct gactaccctc
    13321 ctgggaaata agggaagcga gctggaggga gcattgtgat gatgcgtatc caaggatgca
    13381 gtgcgggtct tatcgagtca ccaccatttc ctcattctct ccattgacag ttttgttgcc
    13441 caagactagc ctgtcgctgt ttttaacgtg aagaagatgc ctggcgcgag ccgtgaggca
    13501 aaggcgccgc tgacgcaatt ttccggaaga cggatcgtct cgatggccga acagaaggaa
    13561 gagaaatcca agctgccgct gacgggcatc gtggccttgt tgttggcggt ggtcagcagc
    13621 ctgatcatct atcaggtgcc gctcaagacg tcgcggccaa tcgacaagga agcggagaag
    13681 gctgtgtcta tcggggcgga tcgggtgcag gcgcgactct ggcaggatcc cttcgaggct
    13741 gtctcgaccc accgacagaa agaggctgct gtcgaaaaac tgcctgattc ggacgcacac
    13801 cacacctttc tcaaactgct gacggcggtg actcaagccg gagcaccttc cgatttctta
    13861 ttgttgccgg tctttgtgga cggaagtccc tatgccagcg gagtcgagtc gaggttgcgg
    13921 gatcgctacg ccctcgtgtc ggccttaggg gcggcgggat atcagccgga gtccggcgaa
    13981 tccatcagat attttgtccg accgggaaac gggcatctcg tcgtccccgt cgaggcgttt
    14041 ctcgggaggc ccaaaccggc tgagccgaag cagtctacgc aggtattggt gctttggctg
    14101 aaggaacagg actttagtcc gaagccgctt ctgtcgctca gtgagctggt gacggaagtt
    14161 caacaggcgt tcaagcagag tacggtgtcc tatcgtgtgg tggggccacg ctcctccggt
    14221 tcgctgggcg ccatggttgg cgaactgacg gctttgtacg gtgccgatca atcctcgggc
    14281 ggatcctccg cggcttctct tgctgcctcc cggcggcagt tggattcgct gaaaggggtt
    14341 caattctatt catcctgggc gacggcggca gatccggttc tgttgggtga acttcaaggg
    14401 ccggatgcgg cacgcggcgt ggagcagtgg ttccaggcgg gcggattgcg cttgacccgg
    14461 accatcggca cggatgccgt gctggcggaa caactggtgg aggagctcaa acgtcgccgg
    14521 atcgacctga cgattccgcg gggggaggct gggccgtgca aggaggcaga ctccgattct
    14581 gccgcggtgt catcccggca ctcggacggg aagtgccgga ttcctcatat cgctctgatc
    14641 tcggaatggg acacgctcta cgggaggtcc ctgccgcgta cattcgtcgc gatggcacgt
    14701 agcctggcgg agacagggcc tgatcgcgcg atgagaggct tcgagtatca ctttaatcgc
    14761 ttgcgcagtg aacagtatcc ggactgggtg caccgctatt cctacctggc cggcctcgac
    14821 ggcgagctgc cgcccaagaa taccgagaag gattccggtg gcgctcagac gaaaggaaaa
    14881 atcggagatg ggggactgcg tgtggagcag agtctggcgg agactccggc cggccgcagt
    14941 cagttggact atcttcgacg cctggttgaa accctcaagc gcgaagaagc gagtaacgac
    15001 ggcgagttca aggccattgg cgtgttgggc agcgacgtgt atgacaagct gatgatcctc
    15061 caggctttgc ggagcgactt tccccacgcc ctgtttttca cgaccgatct cgatgcacga
    15121 ttgacccatc cgagccagct ccagtggacc aggaatctgg tgatcgcgtc ccatttcggt
    15181 ctggaacttc atccgttgat tcagacaccc attccgccgt tccgcgacag ttatcagacc
    15241 gcgttgttct attcggtgct gcgggccgtg aatgatatcg tgccgggccc gggccctggc
    15301 gaactgcaga ttccggcgac ggtgacgttc ccgaagacgg tgcccccgcg gttgtatgaa
    15361 gtcgggcgtc atggtcccgt cgatatcagc ctcgatggac cttacgatgc cggtcggcga
    15421 ccggaggcgc ttccgaacct gcatcttccg cgtcccgatc tggacccgga taccggtgaa
    15481 ttgcgcgtgc tccgcccgca gacgattgcg ttgttcttat ctgcactcag tctcatggcc
    15541 ctctgtgcgc tgttgatcaa cagcgagttg tggcaatggg cgcaatctcg ttgggtccag
    15601 ggagctgtgg tcggccttgt cttgatggtg tgcctgttgc tcggcctgct ctgctgggcg
    15661 atgagcgatc gaacggcggg tgaacccttc tcgctgaccg acgggatcag tgtctggccg
    15721 accacggtgc tcaggcttct cgcattcctg ctctgtctcc tctttctcgg acattcgtgg
    15781 agacgcctgc gggacaacga aacgcagctg agccgccgtt tccggttctt ccgtccacgg
    15841 gcgccgctct cgattcaggc cggctcactc gtcggcattc accactggcg tccgcaaccg
    15901 aggggcgatg cggaggcggc tctgctgtgg cgggaatatc gggcgttggg gagctggaag
    15961 gagcgacttg tgcgcatcgt gccgcaggcg ctgtgttatc tggggttcgg ggccctgttg
    16021 ctggtgatct ccggatttcc ggtgatgcct tgccgcgggt cggcctgctt tacgatcagc
    16081 tatgccgtac tggggttcag tgtcctggct atgacgctgt tgatgtttta tgtcgtcgat
    16141 gcgacgcggc tctgccggcg tttgatcaaa atcatggtcg gcacgactat ttggtggtcg
    16201 gaccgcctgc tcgtccggga ggctgcgaag cggggagtgg accaggccta tgtacatgag
    16261 tggatcgcgg tggagttcat tgccaagcgc acggcggtca tcagcgccat gatttattat
    16321 ccgttcgtgg tggtgttcct catggcggtt gcccggcaca gttatttcga ccggtgggac
    16381 tttccgctcg gtctcatggc gatctttggg gtgaacgccg cctatgcgtt cggcaacggg
    16441 gtcttcctgc gccgctctgc tgaacaggcc aagcgcgcgg ccgtcgccca attgaagtcc
    16501 cggctcgatg gattgtccgg cgaagtcgtc ttcaagaagg aaaaacggga gcaggtcgag
    16561 cgaatagtgg cgctgatcga ggactatcag gagggcgctt tccttccgtt cacccgtcat
    16621 ccgttgttcg gggcgattgc gttgcccacg ggcggcaccg ggcttgtatt tctgctggag
    16681 tacctggcca ccattttttg atatgaacgt ctccggccgg tatgagcgca gagcacagat
    16741 gccggcgacg acgagggctg aatcgatgat aggtgcgatc tccaggagag gcatgatcag
    16801 gagcgtgcgg tcatgacgaa aaaatccaca ggcaaacgcg cacaggtggg aaagtctccc
    16861 gcatcgccgg ggcggaaccg gaccggcacg gatgcgttca tggcggatct taaccgaatc
    16921 atgcgggaac aagtgtttca aagtctggac gatgcgcagg ccttcatgac tcgtctgatg
    16981 gaagaggggg gtggccggct tcccctgcct cgggcgcagg ctccggcaga acgggctcaa
    17041 gaattgatct atcaggcctg ggaggcgcgg actcaacgac aagctgttgc ccttgcccgc
    17101 gaggctctcg aaattttctc tgattgtgcg gatgcctaca atgtgctggc ggaggcagag
    17161 gcacgatccc cgcaggaagc ctgtaccttg tatcagcaag gggtcgatgc cgggcggcgg
    17221 agcctgggca aagccttttt tgacgagcac aggggatact tctggggcat gatcgaaacc
    17281 cgaccgtaca tgcgcgcgcg ccgggggctg gccgattgtt tgtgggctct aggccggaag
    17341 cgagaatcgc tcacgcattg cgaagcgtta ttagagctga atcctgacga caaccagggg
    17401 attcgccatg gattgttgag tcgctatctt acgctgggga acgatactgg agcagctcga
    17461 ctctttcgtg actatgcgca cgacgcctct gccgcgtttc tgtggagccg tgtcttgctc
    17521 gaccttcgac ggggagacca ggtcgcagca aaggagcact tggtactggc gatggacggc
    17581 aatccgcacg tggctgggtt ctttgccggc aagcggaaac cgccggctcg attgccggaa
    17641 cactatagtc ccggagaccg gaacgaagcg gttttgtata ttgcgaactt tgccgaagcc
    17701 tggttggcgt cgcctgatgc gatggattgg ctgacggatc agttggcgaa ctgagcggag
    17761 cgactggtgt cgaatcaccg tgtggatacg cctctctcag cattcccgtc cttgcgtcgc
    17821 gtcatcagac ctggtacagt gcaggtgttc gcgtaagacc ttctttagcc gggggcggca
    17881 gcccttgaat ctttgaagtc ttacgcgatt gttgtttgtg cccgcagatg tgtcgtctcc
    17941 gctgagccta ccgccgaagg ttgttcctcc gtcgttcgca ctgccagttc gtcatcgtcg
    18001 tgggaatctt tctttacctg ttctagaggt gcatcgttgt ttcacgaatc ctgtttcgtt
    18061 ctccgggcat tcttccgcca tgcggcaggc agcctgtatc acatcgtcat cgtcgccatg
    18121 agcgcaggca ttgcgctact cctgccggcc ggagccggcc ggttcctgtc gttctggtcg
    18181 catgtcgaac atgacaaatt ttcactgatt gccgtcgaga tgaccgccgc cgttctgttg
    18241 atgggctgct tcaactatgt ccaccgcagt ctgcgggatc gggcgcttgc cgaagctgcg
    18301 cttggcgccg gcctcgtctc tttctttccg cgacgcgggc cacaggcgcg ccggcgtatt
    18361 caagcgttga aagatgagca gggtaccggt cgttccgtga tggtgatcgg atccagcgga
    18421 gccgggaccc tggtggatca agtcggtgat ctgtcgtcgg tcctcgacaa atgcctggag
    18481 gccaagatcc ttctggtgaa tccgttcagt caggatgcgc gcgcccgcat gcaggcattg
    18541 gcccatcctg cttgcacgta cccggccttc cgtgaggagg ttcggcagag catcgcgttg
    18601 ctcaagcgtc tgaaggcgat cggcaaggtc gtgaagctga agctctatgc cgatgctccg
    18661 ctggtcaaac tggtgatcct cggtgactat ctgtggctgc agcattacca tgccgatctg
    18721 gatattcaga atatgccgga gtatgtcttg cagcggaacc gcaaggagca cggtttgtat
    18781 acgttctacg cccactactt catgcagcga tgggagagcc ccgagttgcc tgaatacgat
    18841 ctggataccg acgaactggt gtaccgcagt cgaaccggtc aggagatccg gcgggagtcg
    18901 gtggagatcg gggccgctcc ggttgccgca cattccgcgg aaactgcgcg ggtcgatacg
    18961 cgcgaggcgt ttcaattgct gcacgcgccg ggtccgtacc tgccggctcg cgggtacgac
    19021 gtcgagtagg gcagttgttc cttcattcct caccacctgt tcggaacatc ggttgttccc
    19081 tgccgagaca tcgcggtcgc tgtggtatta ctatggcgct atggcggtca cgtcgatgca
    19141 tcgatgtatc ggcccggata gacgaggggg aggagtgagg gaccatgggt aatggagtgg
    19201 aacgacgcgc agtctcgccg atgacgacgt tcttttcata cggattccgg ccgtttttcc
    19261 tgggggcttc gctgttcgcc ggtgtggcga ttcccgtctg gattctgatc ctggctggaa
    19321 ccgggaatcc ggtcctgctc tccgcctctc gcgattggca tgtgcacgag atgctcttcg
    19381 gttttctccc tgcggtgatt gccggtttcc tcctcacggc ggtgcccaac tggacggatc
    19441 gtccggcgat ccagggccgc gagctgatgc tgcttttcac cctgtggctg gccggacgtg
    19501 cggccatggc gattcccctg gtggtaccga ttttcgccgc gattgtggat gcggcgtttc
    19561 tcgtggcatt ggtgggtctg ttatggcggg aacttgccgc cgggaacagt tggaaccatg
    19621 cgccggtggc cgtggcgatc agtctgtacg ccgccgcgaa tgtgaccttc catgtcctca
    19681 cagtgagggg tgcggagacg gaccttgcgc tgcggctcgc gcttgccctg gacctctttc
    19741 tgctgacctt catcggcgga cggctcacgc cgaactttac gcgagaattt ctggcgggtc
    19801 aggggaagaa agaacgacct gcgccgtttt cccgcttcga cgcactctcg atcgcgctgg
    19861 tcctcatggc cgcgctggcc tggacggtgc tgccgttggc gtcggcgacg ggctggatct
    19921 ttctcgctgc cgggaccgtg aatctcgttc gtctcttgcg ctggtacggc tggctcacat
    19981 ggcgggaacc gctcgtgctg agtctgcatg tggggtacgg ttggctcgcc ttgtcgatgc
    20041 tggttctcgg gtgcgctctg ctgggaatcg ggctcgcgaa agaagatgcg gtgcatgcat
    20101 tgaccaccgg cgccgtcgga tcgatgaccc tggcggtgat gacgcgtgca agcctcggcc
    20161 acacggggcg gcccaaacat gccggtcccg caacggtctt catctatggg ttggtgatcc
    20221 tcggcgcgat gttgcgtgtc ttcggaccag gcaccggcct tccgacgaat ctggtgcttg
    20281 gctccgccgg agccgcgtgg agcggggcct atctgctgtt tgctctggtc tatgggccgt
    20341 atcttgtccg acccagcctg gatgagtgag gggagggggg aggtgccggg ggcctgccga
    20401 tcggcaacgt gttgaggtgg ttgacgcggt gcaggtggtc gggaacccgg agggaggacc
    20461 gtgctgccgg ttagagttcc tcgccgtact tcaggaaatg ctgctccttg gcgccgtcga
    20521 tccaattgtc gtgtcggatg cgatcgggga acgtgttgag ttccgatgcc atgtgttcga
    20581 cgtcttgttc cttccactcg gcgatctgcc ggaacgtgaa catccccatg cgattgagga
    20641 cgcgctccat caccgggccg atcccgtgaa tctgtttgag atcgtctttt tgggccgggt
    20701 gaccttttcc tgcgctccgt tttgtggtgg cgctaccggt tgatcccgac tcggtggctc
    20761 ccgtcgactt ggcttgcgcg ctgaaccgct gggctacacg atactcgacg agtcgtttgc
    20821 ggagcgctcc gatctcttcc tcccgctcgc tcacatccat cacacgacgc tcttcggcct
    20881 cgcggagcgc aaggtctttt ttctccacct cgccccggaa catgtccaac tcttcgaccg
    20941 ttgcccgcag acgctgaatc tccgcgtccc gttcctgcaa tgtctctccg aatcgcgcga
    21001 ggtcttcctg gctctgcgtt cgcaggttgg cgaggtccag ttcgattgcg ctgaggcgtg
    21061 cctgggtctg tgcgagttgc tgttctttgg tctgtagtat ggctgacgcg cgggcgcgat
    21121 cctgctcctg ttcgcgctcc aatcggcggc ttttttccag gaacggttcc aactcttccg
    21181 cacgggtgcg cagccgggcg atctcggcat ctttcggcgc cagttgctcc acccaggttt
    21241 ttaaggtggc aatctcctgg tcgcgagcga tcaggacatc ttcggcgttt tccatctgcg
    21301 cctgcatctc ggcaaatcca gtcgattgtg ccttgatcct ggcttcggct tcttcgacct
    21361 cgcgtcggag ggtatcccgt tcacgttcgg cggcatgcag gcgtacaccc ttttcccgga
    21421 cttccgtctg caaggtggtg agttgttcga ccttggtggc gagatcggcg gtcaggtctt
    21481 tgagcgccgc ttccgaggtg atcatcttgc cttccagcat ctggacggca gaggtgcgca
    21541 ccttcaactc atggttgagg gtatccagtt catgttcgcg cccgcggagt cgggtggcga
    21601 tttcggacag ctgctgacgt ttttcagagg tggcaaagct gcggagcagc cagcccatca
    21661 tcagtccgat ggtcgctgcc accagcaagc accccaccat ttgcagaatc atggctccca
    21721 tagattcacc gttcctcttt cacacgaaat tcgatgcgcc ggtttttctt cgacgcggcg
    21781 cgcgtccggt cctggctcag ggggcgcgtc gatccataac cgacggcggt cagccgattc
    21841 ggcacctgat gttccacgag gtattgccgg accgcctcgg ctctggtgcg gctcagttgg
    21901 aggttgtagt cgggatcgcc gtaggagtcg gtgtggccgc cgatttcgat gactgcctcg
    21961 ggtgcccgct taagcgccgg caccagtttg tcgaccacgg ctcgtccctt cgaagtcagg
    22021 accgcgctgt tactttcgaa tgcgatatgt tcgccccgca atatctcatc cagcccggct
    22081 tggacttggg ctcgggaggc atgcggtgcg gcggatggct ggctggcggg agcagggttg
    22141 gtctgactcg cggagcctga cgacgcgacg gtcacctggt cttgcacgtg tatagaggtt
    22201 ccgagcagtg aagccacttc atggagcagt tttgtttttg cgtccgcgct tgggactgcg
    22261 ccttttaccg tcaccgtctg gccggagagg gagagggtag cctgatggtg ctggagtgtg
    22321 accgcctgtg tgaggagcgc ggggagggcg gcttcccatc cagcggcctg gccgtcttcg
    22381 atgatgttca aattgtcggt gacacgcagc ctgaggctct tggcgagatc ctgtgcgcgc
    22441 gccaccgcgg cgcctttggc ctcctcgctg gctatcgtgc cggacagtgt cagctggccg
    22501 ttttcaatgt gggcactgaa cgcggagtga ccggtcgccg acgacgtcac cggcaggtgt
    22561 cgagggatac agaggaacgc caataatgtg agggcgaaga gtcctacgcc gaaaataatt
    22621 ccgcgcgaca tgcgcaagcc tttatcgtct caggcacgag gccccgctgc ggcatcgagt
    22681 cgccgtgggc tggtgtcgtt tcaagacttc gagtgttact tgattaagct gatgcgtcac
    22741 ctcatgttgg cgcatctcta tcggcggaag ggtatcacat cgccgatact catgctagcc
    22801 tggattcttc agcagaggcc ggcgtctgcc gggtgatttc gcgtcgcgca gccgtgccgt
    22861 tcggggtcga gttcaggtgt gtccggcttg cagtggccgt gagccatctg gtattgtcac
    22921 ggcgcttttc gtctagctca tggtttccca cccacacgat atcaatagtt gaaggagttc
    22981 atcatggcca gcattgcgcg ggcactgatc agtgtttctg ataagaccgg agtcgtcgag
    23041 atggcgaagg ggctggcggc cctgggcgcc gaagtgttgt ccaccggtgg aaccgcgaaa
    23101 gccttgcgtg aggccggcgt ggctgtcacc gacgtggccg cctatacggg atcgccggag
    23161 atcctggacg ggcgcgtgaa aacgttgcac ccgaaaattc atggcggact cctcggccgc
    23221 cgtcgtgtgc ccgatcatgt ggcgcagatg cagcagcacg gcatcggcaa catcgatgtg
    23281 gtggtggtca atctctatcc cttcgagtcg acgatcgcga agcccaattg tccgttcgaa
    23341 gaggccattg aaaatatcga catcggcggt ccgtcgatgt tgcgatccgc cgcgaagaat
    23401 catgaagacg tgctggtcct ggtcgatccc gccgactacg gccgggtgct ggaggcgctg
    23461 aagtccggtt ccgtgacccc cgcgttgcgc cgcgagttgg ccatgaaggt gtttcagcat
    23521 accgcccggt atgacagtct gattgccggg tatctcgaaa agcaggtgca ggggagcgag
    23581 gtgaagttcc ccgccatcct ctcgctgcaa tttgaacgtg tggagacgct gcgctacgga
    23641 gaaaatcccc atcagcaggg ggcgttctat cgtgaaatga atgccaagga accggcggtg
    23701 tcgcgcggga aaattctgca cggcaaggcc atgtcctaca ataatttcct cgactccaac
    23761 tcggcgctcg aactcgtgaa ggagttcgag cagaccgcgg tggctatcat caagcacaat
    23821 aacccctgcg gctgcgcact cggtgcgaca ccggtggacg cctatgtgaa ggcgcgcgcg
    23881 accgaccccg tgtcggcgtt cggcggtgtc attgccttca atcgtccggt ggatttggct
    23941 gcggccaagg aaatcacctc gaccttcgtc gaggtcgtca tcgcgccggg cttcgccgag
    24001 gacgcgctgg ctgaactcaa gcgcaagaag gacatccgtc tcctggacgt gggaccgctg
    24061 agcaaggcga ctgcggaggg gtacgatctc aagaagctgg tcggcggatt gatcgtgcag
    24121 gatcgcgatt tgggcacgat caaggacatc aaggcattgg cggttccgac ctcgcgcaag
    24181 ccgaccgagg aagaatatgc cgcctgcgcg tttgcctggg tggtctgtaa acatgtgaag
    24241 tcgaacgcca tcatctacgg gcgtccgggc gaaatcgtcg gaatcggggc cggacaaatg
    24301 agccgggtgg actcggtgaa actggccgtc atgaaagccc agtcgcctgt gaagggctgc
    24361 gtgatggcat ccgatgcctt ttttccgttc cgtgacggta tcgacgcggc ggcggaagcc
    24421 ggcattacct gtgtgattca gccgggcggt tcgatccgcg accctgaggt gacgaaggct
    24481 gtggacgaac acgggatggc gatgatactc acgggcatgc gccacttccg ccattgaggg
    24541 acgttgaaac gagcctccaa cttcgttctc ggcgagaaaa aatcctcaac gtagccctga
    24601 ggctacgcct ccggtttttt cttgcctgcg gccttgttgg ccatcccgtt tgaacgccct
    24661 caggggatgt gtcttctttc gcttatctgg acgagcagga aaacgtctcg acatcggcaa
    24721 ttctgacgag cctgccactt acaaaacgct gcttcgtttt tatttaaagt aaaggttgag
    24781 ttgtgaagat tcttgttgtc ggtggcggtg ggcgggaaca tgccatggtg tggaagatcg
    24841 cccaaagtcc gcggaagccg cagatattct gtgcgccggg gaatgccggc atcgaaggtc
    24901 tcgccacctg tgtcccgatc aaggccgatg atatcgaggg gctcaaggcg tttgcgctca
    24961 aggagcaaat cgatctcact gtagtcggcc cggaagcgcc tttggcgttg ggcatcgcgg
    25021 atgagtttcg caaggcgcga ctaaaaatct tcggaccgac caaggccgcc gctcgcctgg
    25081 aatccagcaa gagcttctcc aaagacatca tggccgccaa tcgaatcccg accgcggccg
    25141 cgcgcagctt cgaacacatg gaccaggcgc tggcctatct ggacgagcag ccggttccga
    25201 ttgtggtcaa ggccgacggt ctcgcgcagg gcaaaggggt ggtcgtggct acgacacgcg
    25261 aggaagccaa gcaggccgtc cgcgatgcca tggaaaaatc cctgttcggt caggccggac
    25321 atcgcgtgct gatcgaagag tttctggacg gcgaagagct gacgctcatg gccttcaccg
    25381 acgggaagac cgtggtccct atggtgccgg ctcaggacca caaacgagtg ggagacggcg
    25441 acagcggacc caataccggc ggcatgggcg cctatgctcc ggcgccgatc gccacggctg
    25501 cgctgcgtga acaggtgacg cgtcaggtgc tgcagccgac ggtggatgcg ctggcccgtc
    25561 tgggttgccc ctttcaaggt gtgctctacg cgggcctcat ggtcgtgaag gggaccccct
    25621 atgtgctgga gttcaacgcg cgtatgggag atcctgaaac tgaagtcgtg ctgccgctct
    25681 tgaaaaccga tctggtcgag gtgatggagg cggtggtcga gcaccgtctg gatgaattga
    25741 cggtggaatg gcatccggat acggccgtgt gcgtcgtgat gacgtcgccg ggttatcccg
    25801 gttcctatcc gaccggcctt cccattcagg ggctgcctac tccattcggt gaatcgcgcg
    25861 tcgcggtgtt ccacgccggt acgaaacggg agtcggggcg cgtggtgact gcgggaggac
    25921 gagttctggc ggtcaccgcc tgggggccgt ctttgctcga tgcgcgggcg caggtgtatc
    25981 aagcggtgcc ttcgattttg tttgaagggc gccattatcg aaccgatatc gcgcatcggg
    26041 ccttgccgca caagatctga tccgagcagt tttcggccat tattccatgg ctctgatcct
    26101 tccctttacc gacacaacct gcgatcgcgt tctgcctgat gcccgacagg tgttggatgc
    26161 gcatggcgtt ctcgcgctgc ccaccgaaac gtactacggt ttggcggtcc gtccgacgga
    26221 tgagacggcg ctccgacgtc tcatcgagtt gaaagggcgg ccatccgata agccgattct
    26281 ggtgcttatc ggaagcctgg accaattgcc tcagttggtc caggcgattc ctcccgccgc
    26341 agccctcctg atggaacagt tctggcccgg gcctctgacc atcgtgtttc ctgcgtcgcc
    26401 tgacttgccg gcgctgttga cggcggggac cggaacgatc ggggtgcggc aggctccctt
    26461 gcttcagctc cagcatttgt tgcggcacac cggccccctg accgggacca gtgccaatcg
    26521 ctcctcggaa ccgccgctcg acaacgccgt ggcagtgcag cgcgcgttgg gggcaggggt
    26581 ggatctcatt ttggatggcg ggcacacgcc gggaggtctc ccttctacca tggtggatgc
    26641 gcgcgaccgc ccgcacctgc ttcgagccgg ggccctctcc acggacctga tccgcgcggc
    26701 gcttgcacgg cagggttacg aactttcatc atgatttgat tctcggttgt cggtataatt
    26761 caccctcatg ggaggatcac ttatgctcat tcgttcacgt cgcgcggtga tgggactgtc
    26821 tacgctcggg gtcggtgcga tgcttctgct gtcggggtgc gatttctggc cgccggctct
    26881 ccaggctcag atcgaacagt tgcactcgga agcccaacag gcggccgcag ataaggcgct
    26941 gctgcagaat cagctcaacg ccgcgaacaa ggcgaaagag gaactgcaga ttcgggtgga
    27001 agatttgacg cgggtgaata aagaaaagtc cgcgatgatc gcgaacctgg agcacacgat
    27061 cgccgccgca aaagaacgcg cggtgaaagc cacgaagagc agtgcggtca agccggtcgc
    27121 aaaatcagcc gtgaagaagt ccgtcaagcc gacgcacaag cccgccgcga agtcctcggt
    27181 caggtcccac agcaccaaga aaacaaaacc tgccccggtg aggtaaacac cccgtcgctc
    27241 ctgttgccgg tggagtcctt cccttctgca cgccggactg attcacgttg aaaaaatcgt
    27301 caacggcgtg ctgcctggtt cagggactcc acgtactgca gggggacgcg tcgggctaga
    27361 acgctgtcag cgtaccgtct cgccggcgtc cagcgacctg gcgctcctct atcgtcgcgc
    27421 cgcaggcttc gctgcggctc cattgggcag cgagcttgaa cggcctgagg aggatgtgca
    27481 gggaatgctt gaccgccgcc ggcgctgaag tgcacagggg gggcaaccat ctgtcaagaa
    27541 cctgctaggt gcttgaggac gatgaggagc ttgagcctga tgtgctcgtg ccggatgtgg
    27601 atgtacttgt actcgtcgat gtcgtcgagc tcggagctgc cgcaggggca ggagcaggcg
    27661 ttgtcgtaga actcgtgcct ccgccttcag aggaagccgg tgcctttgtc ttatccgcgc
    27721 tggacgccgc cggtttctcg gaggtatcgc ttcccgagcc gggtttcatc ttgtccgaat
    27781 agtcggtgat ataccacccg ctgcctttga acatgatggc aggggaagag atcagcttcg
    27841 tcacttcttt tccgcaacgc acacattcct taatgggctc gtccttgatg ctttgcttca
    27901 cctcgaagcg gtgggcacag ctcgtacatt gatattcata tatgggcacg ggtcattcct
    27961 cctctggtca gctagcgtcg cgcgctgtcc tgatgctcga tttgccggga tccgcggcgc
    28021 catcttgtag tagctttgat tgagccaaaa tcaagtgctt cgaggtattc aggtcctctg
    28081 agcttaggac aaccggcgaa gcgccgcatc gatacgggtc agtcctcgct cgatcgcttc
    28141 catggcggtc gcataggata gccgaatgtg aatgtcgctt ccaaacggtt cgccgggcac
    28201 caccgccacc tgggcctcgt tgagtagata ggtcgccagg ttcgccgcgg agccgatggg
    28261 ctgatccttc cagcgcttgc ccaacaggcc gcttacattc gggaacgcat aaaacgcccc
    28321 cgtgggcatg cggcaggtca cgccgggcat cttgttgagg cggtcgacca tgacgcgtcg
    28381 gcgccggtca aattccgtca ccatggtttt ggtaaaggga ttcccgaggc gaagcgcggc
    28441 caccgcagct ttctgtgaaa tcgaacaggg gttcgaggtg ctctggcttt gaatattggc
    28501 catggccgtc agcagcggct ttggtcccgc agcgtacccg atgcgccagc cggtcatggc
    28561 gtaggctttc gagactccgt tgatgacgac ggttcgagcc gccacttccg ggcttaaggt
    28621 cgcgatgctg atgtgctggg tgccatcgta gagcactttc tcgtagatct cgtccgagat
    28681 gatcaccagg tcgtggcgca gggctgcggc cgcaatacct tcgagggttg tccggtcgta
    28741 ggtggcgccg gtcgggttac agggactgtt gacgatgatg gctttggttc gtggcgtgat
    28801 gacggcttcc agcgcttcct ggctgatcgt atacccgtct tcttcccgcg tctggagcag
    28861 gacgggcgtg gcgtcgttga gcagggtctg gtcctgatag gagacccaga agggaaccgg
    28921 gataatcagt tcatcgcccg cttcaagcag ggcctccgcc aggttgtaga gcgaatgttt
    28981 ggcgccgcag gagacgagga tctgggactt gtcgtactgg aggccttgct cctccttcaa
    29041 tttatccgca atcgccacgc gcaattcgtc gatccccgac gacggcgtat acttcgtgaa
    29101 gcccgcgcga atggctgctt ctgccgcggc cttcaccggt tcgggggtat cgaagtccgg
    29161 ttctccggac gcgaaatcga tgacatcgat cccttgtgcc gccatcgact tggcggtggc
    29221 ggtaatactg agggtcgggg aggggacaat tcgtccgaca cgggctgcaa gtttcatcct
    29281 ttgaaactcc gaattcgttc ttggagcctc cgagccactt cgggatgcac aaagtcctgg
    29341 agcgatccgc cgtggctggc gacatcctta atgatggtgg aagacaaata ggaatattcc
    29401 tcactgggca tcaggaagac cgtttcgacg gtttccgcca gcttgcgatt gatgagcgcc
    29461 atctgaaact catgctcgaa atcggaaatt gcccgtaggc cgcgcagaat agcatgggca
    29521 ccgcattccc gcacaaaatc caccagcagc ccttcgaagg tggtgacttc caggttcggc
    29581 agatctttgg tcaccagtcc caccatctcc agtcgttcct tcacgctgaa gaacgggtgt
    29641 ttgcccgggt tgggcgcgat cgccacaatc accttctcga acatccgaaa tccgcgccgg
    29701 atgatatcgc tgtgtccatg cgtgatcggg tcgaatgtgc cgggatagac ggcggtcttc
    29761 atcggggttg ggcttctttc gctgtgacgt gaaatcgtgt gagcgcggtg tcgccgtagt
    29821 cgtaccgttt gacctgcgag aggaggccca gggtctgcgg gatctccgct ttctttccgt
    29881 gctcgatgat caccacagca tcgtcggcca gccatccggc gttccattcc tgcgccattg
    29941 cgatgagctc cggggtcaat tggtagggcg gatcgcagaa cacaatgtcg taggggcccg
    30001 accactgggt tgcccgacgg aaaaactggc tgacctggca ggcgcagaca ttggcggatt
    30061 gttgcaggcc gcattgctca aggttcgagt tgactaggcg cagggcctga cgatcagctt
    30121 ccacaaaggt cgcatgtgcc gcgccgcggc tcagcgcttc aattccgatt gagccggtac
    30181 cggcatagag gtcgagcaca cgagctcccg tggtccgttc gccaaggatc gaaaagaggg
    30241 cctctttcac tcggtcggac gtcggtcgaa tcgcctgtcc tctgggacca agcaaccgtc
    30301 ggccccgatg aagccctgcg atgacgcgca tgatcgtatt cgggtgagag ccattcgtca
    30361 ctggctaaga agtgagagac tctaacacag cgtttttgaa ggggtcaagg tgacgaggtg
    30421 tgatcacgga gatgggagcg ccgaaaaggt cttctccccg ccgcggggcg gggagcgctg
    30481 cagacgtcgg cgaggagtgg gcgtgctttg acgcgtagga agcggctcgc tataattggg
    30541 cgacaccaag tctcggttca cgcgggctga ggaggtggcc aaaattcaaa ggtcgtcaca
    30601 ccaccacatc acacagagaa cagagcagcg aacgcgccgg actagcgggt cgcgacgggc
    30661 tgagcttcgc gagcggcgag gctctttctt cgattcgccg aaatcgtccg atcgatgatg
    30721 gctccacagt taatgcactt ccaggcgtag aaaatgagga agaagtctga gaaccgttcc
    30781 aacatcatca tgcctttgca cttcggacat tccatttgct cctcctgggt ggtgacgttt
    30841 acagctgtgt cacctctcaa gcaagagctg caccaaaatg tcaagtctat gtttttcaac
    30901 tacttatgta tgtcaaccgc catacatgag atgcgtcagg cgtcaagtct ccggcacaaa
    30961 aaggtgcaag ccgacatttt tttgtcgtag gaagtaggca atgacaccca aaaaaacagg
    31021 gcgcgggatt ggaaagtggc ccgagactga acgtccgcgc gaacgtttgc tccgtgaggg
    31081 tgccgaaagc ctctccgacg cgcaattatt ggccattctg ttgcgcgtgg ggcggcagga
    31141 agcctccgcc gtcaaagtcg ggatggaagt actcgatcgt gtcggaggta tcttcggcct
    31201 gctgcattgc agcgccgagg agctctgcgc cattcccgga gtgggccctg ccaaggctgc
    31261 gcagttgaag gcggcggtag aagtcgggaa acgcgcggtg tctgcgccgt tgacgaccgg
    31321 gacgcgcatt agctcgagcg ccgacctgtt taagcattac cacgcacggt tgcgggactt
    31381 gcggcacgag attttcgctg tggtgctgct ggacgccaag aaccaagtca ttcgcgacgt
    31441 gacgatttct gaaggcagtc tgacgttgag catcgtacat ccaagggaag tctttattcc
    31501 cgccatgcgt gcgtctgccg ccggagtcat tttcctccac aatcatccca gcggggaccc
    31561 cacgccaagc caggaagacc gggtgttgac ggcccggctg gtgtctgccg gttctctcct
    31621 gggaattcag gtcctggatc atctcatcgt gggggacggt cgctatgtca gttttgccga
    31681 ccaggggtgg ttgaatgggg aaggcgcgag ggtatgaact gcggcgacag atcgcgctcg
    31741 ctcctttccc ggcggaattc gatcgaatgt tttgagaaag gatggcatca ccggcacgtg
    31801 tctctctaac caggcccaca gcggccgccg actcatcgga ggaggtcatc atgaacgttc
    31861 ccctcaccga atccatcagg aatgtggctg tggtatcgca tgcgggatcg ggcaaaacct
    31921 cgttagtcga agcgctggcg tactgcgcgg gcagtctctc cgctcccggc tccatcttcg
    31981 ccggaaccac ggtgtcggat tttgagccgg aagaaatcca tcgccgaacc tcactcaaca
    32041 ccaccgtcct tcgttgctct tatcaggatc gagtgctcac gcttctggat accccgggct
    32101 cgcccagctt catcggtgag acgcgatcgg cattgcgtgc ggcagacggt gtggtattgg
    32161 tcgtgagtgc ggcatcgggc gtgcgcagcg ccctgcagag gatctggtcg atggtccgtg
    32221 actccgggtt accctgtgtc gtgttcgtca atgatctgga caaggacggc acggtattcg
    32281 acaacacggt ggaggatctg accaaggaat tggagatgac tgccgttccc ttgatcctgc
    32341 cgatgggcgg cggtgcgcaa ttgggcgacg tcgtggatgt gttgcagaat catgtggtga
    32401 cgtcgcagcc ggaccgtccg aaatcgcagc aggggccggt gccgcctgag tggcgtgacc
    32461 gggttgatca agccaggcgt cgcgtcatgg aactcgtagc cgagggagac gaggcgctgc
    32521 tcgaacggta tttgaccgac ggcgcattga cggaggaggc gttgctggag ggactgcagg
    32581 ccggagttca acgcgggtcg ctggtgccca tcgtcggagg atccgccttg cgacacatcg
    32641 gtgtgcactc gctcctgcgc actctcgtcg agctgttgcc ctcaccagtc agtcgcgccc
    32701 gggttgcacc gctgcagggc acagggtcga cggaagacgg gggaccggta aacaggcacc
    32761 cgctgccgac cgatccattc tctgccgtcg tcttcaagac cctcatcgat ccattcatcg
    32821 gccgtctttc ttacgtgcgc gtctattcgg gaacgctgga agcggatacc gcaatgtata
    32881 attccacgcg gcaggtcagg gagcgaggcg ggcatctgtt ctcaattttc ggcaaaaaat
    32941 atacgccgat cacgcgtgcg acggccggag agatcgtggc gatcggcaag ctgaaggata
    33001 cggtgacggg agatacgctc tgtgacgagc atgcgccgat ccgatatccc gggattccat
    33061 ttcccaggcc ggtggtgtcg tttgcgatcg agccgaaatc gaagacagat atcgagaagg
    33121 tgagcctggg gctgcataag gtgatcgagg aagatccgag cctggagttc gtgcgtcata
    33181 tcgagacgaa ggagatggtc ctgagcggca tgggccaatt acacatcgat gtggcgttgg
    33241 aaaaactgca tcgtaaatac ggtgcggacg tgattgtgca cgcgccgaaa attccgtatc
    33301 gggaaacgat tcgtagcgtg gcccaggcac aggggaagta taagaagcag accggcggcc
    33361 atggccaata tggtgattgc tggttggaag tcggtccgtt gccgcgcggc cagggcttcg
    33421 agtttgagaa taaaatcgtc ggcggcgcca ttccgcgcaa cttcgtgccg gcggtcgaaa
    33481 agggtgtcgt ggaggcgctg catgaagggc cgcttgccgg gtatcctgcc gtcgatgtgc
    33541 gggtgaccgt ctacgatggc tcctaccatg tggtggattc atccgagttg gccttcaaaa
    33601 tcgccgggtc gatgggagtg aaaaaagcgc tggaagcggc ccacccgatc ttgcttgagc
    33661 cgctgatgac ggttgaagtc gaggtaccgg ccgaatgtgt ggggacggtc cttggcgacc
    33721 tgaatgcgag gcgaggccgg atcgtcatgg tcgaggcgaa cggccatatt gaaatcgtca
    33781 aggccctggt gccacaggcc gagatgctca actatgcggc gtcactaaat tcaatgacgg
    33841 gaggacaggg gagctacgca atggaatatg ctcaatacga ggaagtgccg cgcgagttgg
    33901 ccggccgcat cattgaggaa cacaagatgg agcgccacgg ggccgtggcg cattgagcgg
    33961 cgggatccgc cagaaggaag gattggcggg ctatccctct taccgatcgg cgggcttcag
    34021 gacgacgtag aatgcccgcc cttcgcgcag gacgcgcaag agaatcgtgt cgccgcgttt
    34081 gaccttggag acggccaccg tgaattctcc gactgtgcct gtgtcgacgc ggttgacttc
    34141 cttgaccaga tcgccttccc gcaacccttc ggcctgggcc agacttcctg cttctacttt
    34201 cgagatcagc acgcctctgc tctcgttcag tcgaaacttc tccgccagac ccgcagtcag
    34261 gtcttgtacg tcgattccca gcttcaagtc cgtcctgctc tgaggaagag aggccgtaac
    34321 caccgcatca cggcgttcgc tcagggccac gttcagcgtg aggcgtttgc cgtcccgcac
    34381 gacttcaaca tgcgtcgtgg agccggggtc taacgcggcg accaggcggg agagtttgtt
    34441 cggggtatcg acgagcgcgc catcgacttt ggtgatgaca tcgcccggct tgatgccggc
    34501 cagagcggcc gggtcccgtt cgaatacttc gttcaccagg accccttcgt tttcatccac
    34561 gccgaatttg ttggccagat ccggtgtcag cggttgcagc ccgacgccca gccaggcccg
    34621 cacgaccttt cccttcgaga tgagctggtt catcacctgc ttggccatgt tggagggaat
    34681 cgcaaatccg atcccctggg cgaaattgat gatggccgtg ttgatgccga tgacatctcc
    34741 ccgcagattg aagagcggtc cgccggagtt ccctggattg atggaggcgt cggtctggat
    34801 gaagttctcg taccgtgaga gattgatgtt ttcccgtccg atgccgctga ccaccccgag
    34861 cgtcaccgtt cgatccagac cgaaggggtt tccgaccgcc agcacccact gaccgacctt
    34921 cactccggtg gagtcaccga accgggcggc ggggaggggg tggtcggtcg tgactttcag
    34981 aacagccagg tcggtgtccg gatccttgcc gacgacttgt gcgaacagtt tggtcttgtc
    35041 ggagaggcga acctcaactt ccgtggcatc acccaccaca tggttattcg taataatgtg
    35101 accgtttgga tcgacgatga cgcctgagcc ggaacctgtg gagttcggaa cccgctcgcc
    35161 ggatccgtcg cgcgacttgc ccagggtctg aatcgggaaa atgctcacga ccgacggttt
    35221 agcctcctcg gccaagtccg tgatcacggt ttggagttct tcgagcatgc gcagcccggg
    35281 cgactctttc cgagggggtg atgcgatgtc ggtagcggcc agtgtgatcg ctgaggtgcc
    35341 cgtcagccac ccggagaaac cgagagcgag aatgagtgcg gtcgcgaagg aagtctgtgt
    35401 cttcatgctg ggcaagctcc ggaggccgaa tctcacgaac ctggttcagt ttgagaataa
    35461 cagattctcg ggcggcaacg gaagcgagga ggcttaggcc gatttcgatg cagccggtcg
    35521 ttggaaatgc ggaagccagt gagccaggtc gtggcgaagg ccgacaagaa tcacaatgtc
    35581 gtcgggccgg agtgtcatgg aatcggagag cgggagcagg aatgggcctc gaaagaccgc
    35641 tacgatgtgg gtcgtcgacg gcaggtcgaa ttcaccgatc ggatggccga tgaccggtgc
    35701 gccacgggcg acgagcacgc gttcgatggc cgcgtcacgc agacccagtt cgcgttgcat
    35761 ggtgagcagg tcctcgtggg ccaattcgat cttgaggtca cggatatgcc gttcgatctg
    35821 ctgcaaggcg gtctgttgcc ccttgatgcg gtcggaggct gtgcggatgg cattttccag
    35881 cgacgggagc gtgccgggag cgaccgacgg tatggcgccg gagggtttgg ttcccaactc
    35941 tgcgatgcgg cgaccgacct gtcgatgaat ggcgtgaatc tggttcagaa tcgtcgtggc
    36001 ctgaccatgg aggcgcagga catggacctt gcggttgacg cgttccgcga tggcgacaat
    36061 cgtctcgtag agtgcgctgc cggtgaggaa cagttctttg cggacgcgag ccaacagctc
    36121 aaaagacagc atcggataaa tgtccgataa gatatattat gtcaacctgc ttgtccgatt
    36181 cgagattccg tcccactcca caaccggacc agattgtctt tgtgccttac ccagattaat
    36241 aggctgacga cacaggcaaa cccgatgaaa gtccaagacc ggtgaaacag caacgccaca
    36301 attggaaaca tggcaaaggc gaacagtgcg ccgcctgacg aatatttcca caggaatacg
    36361 gcgccgaccc acaaacccat cagggtcagt ccgatccacg gagcgactcc cagggcagcg
    36421 cctaatgcgg tagccacacc ctttccaccc ttgaatccca gaaacgccga gtgcagatga
    36481 ccgatgaccg aggccagcgc gacccagaga atggccgact cctgatgcaa caacatcgtg
    36541 ccgacccagc cggccaacca gccttttccg atatcgccca gcagcgtcag gattcctgcc
    36601 ttttttccgc tcacgcgcag gacgttggtg aagcccacgt tcctgctgcc ggcgctgcgt
    36661 gggtccacag cgcccagaag gcgtgacacc acgactccaa aaggaatcga tccaaggaga
    36721 tagctggaaa ggatgagaaa cccgatgagc cagggactgt ccatcaatcg ttgcccggat
    36781 agccggatac gagcgtcggc cgaatcgtcc ggttagagac ctttcgctcc gacctgacgc
    36841 cagaagctcc agacatattg tgcgcctgac acatagccca ggatgagtga cagatacaag
    36901 gtgacaatgc cggccagatg cagattaccg atcacttcca ccacggtccc ttcgagcacc
    36961 aggaggacga tggcaatgac ctgcagggcc attttgtatt tgccggtgac ctcggcggac
    37021 atgatcaaac cttcggaggc cgcgatggcc cggaggccgg tgacggcgac ttcacgcgca
    37081 atgatcagaa tcgccaccag cgcgctgacc cgatcgacct gtaccagcag gatcaatgca
    37141 gacaacacga gcagtttgtc ggcaatcggg tctaacagtc gtccgagttt ggtgatctgg
    37201 ccggtcttgc gcgcgacgta tccgtccagt aggtccgtca cggccgcgac gacgaatacg
    37261 atggccgcgg ccaaggctcg atccggcgtc gggtcgatga gcagcatgac gaagaccggg
    37321 atcaagagaa tccggacgag cgtcagcacg ttgggcagat tgatgttgct ttcctgccct
    37381 gccgaccgca tcagaaccag cccggcttgt ttccagtttt ctgccataaa ttcttcgaca
    37441 aacccttcga cgcgcagcgc gtgatggctc ccctacacaa tgccgtgttt tagcaggtca
    37501 tgcaagtgaa tgacacctgc caaacgatgc ggcctctcca gaactaccag cgccgtgatg
    37561 gaaaaccgtt ccatcagcgc gacggccgtc gtggccagtt cgtccggtcc gatggttttc
    37621 ggttggcggc tggcgagatc cccggcggtc actttgctga agtccccgcc tgcttgaata
    37681 aagcgtcgca aatctccgtc ggtcacgatt ccgtacagtg cccccttggc gttgaccacc
    37741 gtcgtcatcc cgagtttctt cgacgtcatc tccaggatcg tatccgcgcc ggacacagtg
    37801 tcgcgcacac gcggcaggtg atcgccatgc tgcatgaggt cccgcacttt caccagtagc
    37861 cgtcgcccta aggtgccgcc ggggtgaaac tgcgcgaaat cgtcctgttt gaaaccgcgc
    37921 ttttgaagca gcgcgactgc cagggcatca cccatggcca gggtcgcggt ggtgctggcc
    37981 gtcggggcca atcccagtgg gcaggcttcc tcatcgaccg atacatccaa cgtgacatcg
    38041 ctgtttttcg ccagcgtcga ggccattttc cccgtcattc ccacgaccgg aatgttcatg
    38101 cgtttcacga agggtagcaa ttggaggact tcctgtgtct cgccgctatt ggagatggcg
    38161 atcaacacat cccgtcgagc caacatgccg aggtcgccat ggacgccttc cgccggatgc
    38221 aggaagaagg agggggtgcc ggtgctggcc aatgtcgccg cgattttctg gccgatcagt
    38281 ccggacttac ccataccgga aatgaccacc ttgccctggc attcatagag aaagttgacg
    38341 gccgaggcga accggtcgtc gaggcgagcc atcaattcct gaacggctcg cgcttcgatc
    38401 tccaagacac gtctcccctc ctgcacgctg gcatcctgtt gagggcgtcg agcggcgcgc
    38461 ttgggtgtgg gtgatgtggc ggacccggtt attttgcggc tgcgtcccat atacgtagta
    38521 cccgttcgag aagtgattgg agcgtatgca gcgggaccat gttcggtcca tcagacaacg
    38581 cggaatccgg gtcggggtgc acttccatga agaacccgtc gcagccggcg cctgccgccg
    38641 cacaggccaa tggttccacg aattcgcgct gaccgctgga tttcgtgccg cctccgccgg
    38701 ggagttgcac gctgtgggtc gcgtcgaaga ccaccggata gccgaaccgg cgcatgatgg
    38761 gaaaggagcg catgtccacg accagattat tgtagccgaa ggaggaacca cgttcggtca
    38821 acacgatgcg tcggttcccg ctgtcttcca ctttcttcac cgcgttgccc atttccggcg
    38881 gggacagaaa ctgtcccttc ttgatattga cgaccttgcc ggtcttggca gcggcgatca
    38941 agaggtcggt ctgacgacag agaaaggccg gaatctgcag gatatctacg acgcgaccgg
    39001 cctctgtggc ctgctcttcc gaatggacat cggtcaacac cggcacgccg atgtgttgct
    39061 taaccctggc gagcacggcc aagcccttct ccaagcccgg tccacgaaac gaggtgatgg
    39121 aggtgcgatt ggccttgtcg aaggatgatt tgaagatgta gggcatgccg atcgctttgg
    39181 cgatgtcggc aatacgccct gccgtgtcta tgacgagctg ttcgttctca atcacgcagg
    39241 gtcctgcgat gagaaacggt cgatttcccg ccccgatctt aaattgtcct aagtctactt
    39301 cgtacgccat agtctcctag caggatgcgg aacaatcccg ccagctgcgc gctctcgcat
    39361 cgcttagcgg ccttgctgga cggccatttt acgcatcctg cacgttactt tgataccagc
    39421 ccgccacctg ggctgttctc accgtagggt ggtgaatcat gcgtttcccc agtctgctga
    39481 atgtccgcca atcacgggat gctcaaactg atctttcagc aaggccgcag acgaggcaga
    39541 accggaggcg tagcggagct acgtcgagga ttgtgccgag tcgagaacgc cgccgaaagg
    39601 cagtttcagc atcccattta gtggccgcac ttgcgccgca gcgccgcccc cacaaacccg
    39661 ctgaataacg gatgcgggtg atgcggccgt gacttgtatt ccggatgaaa ctgcgtagcc
    39721 aggaaccagg ggtggttctg caactcgacg atttccacca ggcgaccgtc cggagacagg
    39781 ccgctcaaaa tgagtccctt ggccgtcaat tgctcgcgat aggcattatt gaattcgtat
    39841 cgatggcgat gccgttcgcg tacctcgctg accccgtaca tcttctgcgc cagcgtcccc
    39901 tcgcccagct tgcaggcata ggcgccgagc cgcatggtgc cgcccttgtc gttgaccgac
    39961 tgttggtcgg acatcaggtg aatcaccgga tgcggggtct tttcatcgaa ttccgcgctg
    40021 ttggcaccga caagccccgc gacgttacgc gcaaattcga tggtggcgca ttgcatgccc
    40081 aggcagaggc cgaggaacgg aatctgatgt tctcgcgcat attggatcgt gacgatcttg
    40141 ccttcgatcc cccgtgcgcc gaaaccgcct ggaatgagaa tcccgtcggc ttcacggaga
    40201 atacgctcgg tcccctgacg ctccacctct tcggattcga tccagttcac gttgacgcgg
    40261 gtttcatgat cgatgccgcc gtgcaccagc gcttccgcca ggctcttata gcattccttc
    40321 aagccggcat attttccgac caacgcgatc gacacctcgt gtttgggatg cttgatcttc
    40381 tgcaccatcg catcccactc gcggagattc ggcgggccgg tttccagctt gagcagtcgg
    40441 acgatcagtt catccagtcc ttctttgcga aacacgatgg gcacttcgta aatggtctcg
    40501 acatccttag ctgtgatgac cgcgtctttt tccacgttgc agaacatggc gattttcgcc
    40561 ttgagctccg gcggaagata gcggtcggtg cggcacagga ggatgttcgg ttgaataccg
    40621 atttcacgca gcttgttcac ggaatgctgc gtcggcttgg ttttcaattc gccggcggct
    40681 ccgatgtagg gcaccagcgt aaggtgcaca tagagaacgt tgtcgcgtcc cacgtcgtag
    40741 gggatttgcc ggatggcttc caggaacggt aggctctcga tgtcaccgac ggtcccgccg
    40801 atctcgacga tcgtgacgtc catgccttgg gagattcgca tgatgcattg cttaatctcg
    40861 tccgtgacat ggggcacgac ttgcaccgta ccgcccaggt aatcgccgcg ccgttccttg
    40921 gtgatgacgg cgtggtaaat gcggccggtg gtgtagttgt tctcgcgcgt cagagtcagg
    40981 gaggtatacc gctcatagtg acccagatcg aggtcggttt ccgccccgtc ttccgtgacg
    41041 tagacctctc catgctggta cggattcatg gtgcccggat cgacgttgat gtagggatcg
    41101 agcttgagga acgtgatctt cagcccgcgg ctttccagca gattgccgat ggatgcggaa
    41161 gccagcccct ttccgagcga cgatacgaca ccgccggtca caaaaatcag tttgctcatc
    41221 gtggcacctt ctctcgtcat gagtgagtga tgccgagtcc ggttgcacgg ctgtgaagtt
    41281 gtttcagtgc gccgggcacg tcctcggggc tatcgatgcg caaggaaccg tgggtggttt
    41341 cccacacgcg gatacgcatt ccgttttcca aggcgcgcag ttgttcgagt ttttcggcct
    41401 cttccaaagt ccctgtcggc aaggcagtca gacgttgcaa tgtttcccgg gtatagatgt
    41461 acaagcccag gtgaatgaaa tgcagtcccg gcaccgcagc ccgcttcgga tcgtcccgca
    41521 ccaacggaat cggcgcgcgg gaaaaataca aggcatagcc ctcgcgatcg gtagtgactt
    41581 tgaccacgcc cgggttgtgg agatcctgat cggaatcaat ctgtcgtttc aaggtgccca
    41641 tcgtggcctc gctggccaga aagggctcga ccaggtcggt gatgagatcg ggatggagca
    41701 ggatctcgtc tccctgcagg tcgacgaaac agtcgccggt gcggctgtcg gcgacggcgg
    41761 cgacgcggtc ggtgccggtg cgatagggct ccgtcatcat cacgacctgt ccgccgaagc
    41821 gctgaaccgc cgttttgatg cgctcgtcat ccgtggcgac cagaacgtca tcgaccgcgc
    41881 gacaagcctg tgcctgctcg tagacatgtt ggatcatcgg tttgcccagc aactcgacca
    41941 gcggtttgcc cgggaatcgt gaagagccgt accgggctgg aatcaccacc gtcaccgagc
    42001 gtttcacgtc agttctgtcc cagggcatcc gtgagttgcg cggccgtaac cgtagcggta
    42061 ccgatcatgc cgacgacgac cccggcggct tggttggcaa gcaccgctgc gtcacgcatg
    42121 cttgcgcccg tcgagagcgc cagcgcgagt gttccgacga cggtatcacc ggcaccggtg
    42181 acatcataga cctgtcgggc gcgtgtgggg atatgccaat ggtcgccatg agcctggtac
    42241 acactcatcc cgcgccgcgc gcggattata acggcgggcg atttctttga caaccttggg
    42301 a
//
LOCUS       DAORRQ010000040        41839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_62_length_41839_cov_67.124545, whole genome shotgun sequence.
ACCESSION   DAORRQ010000040 DAORRQ010000000
VERSION     DAORRQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 41839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41839
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62_length_41839_cov_67.124545"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..762)
                     /locus_tag="PK224_13225"
     CDS             complement(25..762)
                     /locus_tag="PK224_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16750.1"
                     /translation="MKINRTMTAQGMRSHLDFRYRGVRYRPVLGYDLMSDQEHAAMVQ
                     ILASIQSHVGGVAGDQGPTFAEFTPKYLAKLQSKGLRALDRPSTILHRHLVPFFGVRP
                     LSSLKLQDGVGYIAHRRGQDAADGTIKRECGVLLGLLNYAVANEDLDKNRLLSLDVPS
                     GAKRERVAEAWELFRIYHAAAPSIQRMMMLALLTTLREAKIIALHHEWLIQRGDGEWL
                     IPAPGSRLKGVPKELPLSEGAPQIRTV"
     gene            complement(974..1255)
                     /locus_tag="PK224_13230"
     CDS             complement(974..1255)
                     /locus_tag="PK224_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB16751.1"
                     /translation="MTLLTLIETAELLRVSPRTVRRLPIPRAYVGGQLRYDLSDLEAY
                     VASRKQGGQDRAPSRPPASLVIKGTLSAQTTSGRIVQHRNPLFAIRGTR"
     gene            complement(1303..1881)
                     /locus_tag="PK224_13235"
     CDS             complement(1303..1881)
                     /locus_tag="PK224_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16752.1"
                     /translation="MGTLGALQNISGAVEGPSLTSLKTAQEVAEQVRLSVGRIRELAE
                     AMLLPHFRIDGGEPLFCPTTLKRYVRQHLTVMCEGAPLPLNLRPIVQRPLTQPVPVAL
                     AAVQDRLCDCPALDIPPCVYFLIQAGAVAYVGQSRHLPSRLAQHSQSGKRWERVLFIP
                     VPESELLRVEAQWIAALQPPLNKTSLTRNPEE"
     gene            complement(1904..2053)
                     /locus_tag="PK224_13240"
     CDS             complement(1904..2053)
                     /locus_tag="PK224_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16753.1"
                     /translation="MAMVKLLIQIPQAIKAKLDALRKQGTTSSGFIRSLLEREFQKRD
                     EQNRS"
     gene            2161..2337
                     /locus_tag="PK224_13245"
     CDS             2161..2337
                     /locus_tag="PK224_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16754.1"
                     /translation="MMKSQEKAEKPRKTFGLSLNRELMLEIQHLALDQDRYVNELLEE
                     AAKDLLKKYKEKRH"
     gene            2359..2604
                     /locus_tag="PK224_13250"
     CDS             2359..2604
                     /locus_tag="PK224_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16755.1"
                     /translation="MTSNELKEHIRLMIQPVDLEGLIAQGVLAKAGAWYRIMNMKKLP
                     KSVSMHISEISRDSQGRAKIKFAKVSDRQAVSLRKFL"
     gene            2679..3845
                     /locus_tag="PK224_13255"
     CDS             2679..3845
                     /locus_tag="PK224_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16756.1"
                     /translation="MQTLTDEVFRRLLIAKGLLGKIRFSNSAQPDRVMLASHILAAHD
                     AAELAAAAIAHHLQAGADKGKTFLMDYLPLIEKKAHEGRQIEGRDYFSQLNDVRVALK
                     HKGILPDAKDWRRVGERTWGYISVWCDEYFGLSLDGLDESGLISDPNVKAHYTTARAT
                     FEKGLYKECFEHLALACQKLFDENDALRNLSAGEAKAEDAIKLAAYGVHANDYLALQQ
                     FLPKVIISSQDPPACHWEQNLYGHPANWTERNAEFGLRAFVDLTLRIQDADWIPGPYA
                     FHLIYDYKITAIDETVAVVRERTPRSLLDAVIEDKDEVRILKKGNSIRCRVIVQNPIS
                     LKLSDFAPDTLIIVDDENITHRLKRSGVQITCVPREDPIIGKYFPDLSEIPYES"
     gene            4203..4802
                     /locus_tag="PK224_13260"
     CDS             4203..4802
                     /locus_tag="PK224_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16757.1"
                     /translation="MAAEYTSHDYSFHGIVSRLAENKTYLFVYYSGVVLTSIGAGWIA
                     RRIVWRDELDVKWPWLRYRSEWLYKIMGRGGLKDVPWKDTESWIDILSEQDALEPGKA
                     VLYRGLAAGYTTEDNGALRDVILTDVKRTIGKKNSNGDVVWTHVGGRFFVMSYSKVRN
                     MNITYEQYSRKLLEQISDLPAGPVPFGPEEEGESPSSSQ"
     gene            complement(5319..6395)
                     /locus_tag="PK224_13265"
     CDS             complement(5319..6395)
                     /locus_tag="PK224_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB16758.1"
                     /translation="MGLFKRNKVWWMTFVYQGQQIRRSTECTDRRLAEAVLGKIKVKL
                     VEGRYFDRLEEQERTFEEMVERYVAERVVGASRHGERRARGILAHLLPTFGKLTLVRV
                     TPKEIAAYKWKRQQAGAAPATIVKELALMKTAFNVAIREWEWCRDNPVCRVSMGKVNN
                     ARVRYCDDETLAKIYQACPMWLRPIVMLARYTGLRRENVVCLQWEQVDLVRGLIILEQ
                     TKNGDRLGIPLCDPARQTLEVLKPCRPLASGSVFLQHTPDREAVTPDMVTTAFRRACE
                     SVGVTNFRFHDLRHTFASALVQRGVDLYRVQRLLGHRDGRMTQRYAHLAPENLREAVQ
                     VFKDDYHKFSTMAGCDRPRLVLSV"
     gene            complement(6382..6696)
                     /locus_tag="PK224_13270"
     CDS             complement(6382..6696)
                     /locus_tag="PK224_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB16759.1"
                     /translation="MLTVKELSAWLNIKQSTLYLWVSQNKIPCHRIHGLIRFDPDAIQ
                     AWLKGFGAIPVKPFPLPIHDDSRDLNHLIEAAKSEVYTPRHGETRPKSGLIQKEDADG
                     AV"
     gene            complement(6740..7750)
                     /locus_tag="PK224_13275"
     CDS             complement(6740..7750)
                     /locus_tag="PK224_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB16760.1"
                     /translation="MDSTFTLSIDWLAFTVLASNSQETMKVLGGEWNRAKGGFRGYPL
                     SWIKTDGLRGVGKLGTNAPRRPNEIHLDLSGGLVSALTLDQIRVLLKWVHAQQGHVTR
                     IDCALDDRGATVSVSAIREAVAAGQCVTRAAQVRHIVSNLTHGNGETTGETLYFGSPQ
                     SQTLLRIYDKRLELKSKEQENWEEYGTRWELELKKDRAEQCARALASLDEADWKELVV
                     GLLRSYVDFRQIPKDAEDEERYRAPVLEWYALLTEGFQKGRLAQEKQVQTLQNVKRWV
                     SDTLTPMLAVICATPGGEEWLQHEIVRGISRWKDRHRNLLKQPPTRFHTSSAGGNAGS
                     PC"
     gene            8391..8627
                     /locus_tag="PK224_13280"
     CDS             8391..8627
                     /locus_tag="PK224_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16761.1"
                     /translation="MIARLIFLLVWASQGLLALSQVHAEQMEPGEFAQGVEYKLLMSK
                     YEMVCKHMLTLFNQDLAKFGTEKYDEHEEFRSIG"
     gene            9234..10268
                     /locus_tag="PK224_13285"
     CDS             9234..10268
                     /locus_tag="PK224_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035718769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HRB16762.1"
                     /translation="MTTEQKIIKTKVGVLELAKHLGNVSKACQVMGYSRDSFYRFKEL
                     YDTGGEAALQEISRQKPLLKNRVAPEVEQAIVHMAIEQPAWGQLRVANELRKRALTIS
                     PAGVRCIWLRHDLETMRKRLKALEAKVAQEGLVLTEAQVVALEKAKADKEAHGEFESE
                     CPGYCGAQDTFYVGTLKGVGRIYQQTFIDTYSKVGFAKLYDRKTPLTAAELLNDRVLP
                     FFEHHEIPLNRVLTDRGTEYCGAPERHEYELYLAVENIDHTRTKTRHPQTNGICERFH
                     KTMLNELYRVAFRKKIYRTLEELQTDLDAWLADYNNQRPHQGRWCFGKTPMQTFIDSV
                     PLAKEKILAA"
     gene            10273..10560
                     /locus_tag="PK224_13290"
     CDS             10273..10560
                     /locus_tag="PK224_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16763.1"
                     /translation="MKTRWLSDEVLSYTDENRIAHNLFARLLRAVLMTLNKLTGIAGS
                     KLADLSENYEARADYQVAFFFYSFAALLLFLFLAAAILFIMQKIVSIKSRN"
     gene            10726..11562
                     /locus_tag="PK224_13295"
     CDS             10726..11562
                     /locus_tag="PK224_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16764.1"
                     /translation="MGTSVGSSPVSQDGRKWTSNDLNGPVHVMRQQTTNFRQVGDEWI
                     EEEPRDEYVSTYDRTKLVLEKEWLQPILPPLPQKTFTCFGRDPMGKIVQAFQCDASHS
                     PIDGFREVFLYDSKGYLAEQQMIYLDEIPSRASREVYQYDETGLKVSEREYDAHGALT
                     RVVTLERDPTKHALSVSARTGDGMLWSKSRHICDDKGRPIESVNLDPDDQILSTHRMT
                     YDSYGNWIERTSSGQGLDLREVVVYEYDRWGNWTKKTSRVIGTGGESRWTIRRTIEYF
                     PD"
     gene            complement(11965..12381)
                     /locus_tag="PK224_13300"
     CDS             complement(11965..12381)
                     /locus_tag="PK224_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRB16765.1"
                     /translation="MPQPAVLLDTDILSELLKQHPLVLQRVRTYLAEHERLAFSIITR
                     YELLRGLKAKQARTQEAAFTLLCQASLILPITDQVVERAATLYGDLHRQGALLPDADL
                     LIAATALDAQRPLITNNLAHFQRIAGLSIESWKRSD"
     gene            complement(12381..12617)
                     /locus_tag="PK224_13305"
     CDS             complement(12381..12617)
                     /locus_tag="PK224_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HRB16766.1"
                     /translation="MRQTIKARYHDGVLQPLEPLALTDDAEVQVTVDTEMAVNAEEIL
                     RRAALVYQGLTADDIAQVETIALNRQQFFREPAA"
     gene            complement(12766..13185)
                     /locus_tag="PK224_13310"
     CDS             complement(12766..13185)
                     /locus_tag="PK224_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRB16767.1"
                     /translation="MRVVLDTNIFVSGLLSAAGPPARIIQAVLQRRLIPIMSPATFAE
                     LEAVLRRPKLQRAFTRAGVNITSFLATIQAEVQFVDPHPTNTPLRDAQDRPFLDLMAT
                     TPPPQYFVTGDKDFEASHYSGVPVISAAEFARLLAHR"
     gene            complement(13169..13423)
                     /locus_tag="PK224_13315"
     CDS             complement(13169..13423)
                     /locus_tag="PK224_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16768.1"
                     /translation="MKAVKYTKEGVVIPSSWMKGWGKAVVAHRDSQVLILESPARAAS
                     RKKLARMVSKLRRATRELGVTPEQIAAEVAAVRRERARRS"
     gene            complement(14243..14974)
                     /locus_tag="PK224_13320"
     CDS             complement(14243..14974)
                     /locus_tag="PK224_13320"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB16769.1"
                     /translation="MALATLLLGNPVPLATAEDEAQVQESARLIAVLLDSGRVAIGRN
                     QDLINDPARGDKGFTPEVFAEQMIALFKERTGHDLRNLSTAQVPALAKPLLERLLEES
                     KKTVATYQTAINVPGLKYKGLIPATFGTETGARFQLWSGIYLKQTAPEGLLRNPKNKP
                     DAFESAALHKMEDPSFPRNGEQIVSEVVDGGKSVRVLLPLFYGKACLSCHGTPKGERD
                     VTGYAREGAQEGTLGGAISVKLPLP"
     gene            15571..16083
                     /locus_tag="PK224_13325"
     CDS             15571..16083
                     /locus_tag="PK224_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HRB16770.1"
                     /translation="MNKGLLIALTGVLMGVQAGVAHAVNPDNGPGCGLGKLAWADYKT
                     PKNIAPQVMMATTNGTFGSQTFGISFGTSGCTNDGKVMAGQQTNVFVAGAFESLSEDM
                     ARGGGEHLATLATLMGVPSEQQSTFFALAQQQYGALVGAGELSSVAVVKALQEAMKEQ
                     PVLAQASGIR"
     gene            16298..17464
                     /locus_tag="PK224_13330"
     CDS             16298..17464
                     /locus_tag="PK224_13330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRB16771.1"
                     /translation="MTQPSHRLLLLTIVILGIGLYSLDLYLPLGIGNGVLYGGLVVLS
                     LASPDRKIPLLVAGACSVLAISDVFLGVRFPNVPLWMGISNRLFSLVAIWFPVVYAFQ
                     RRKIEEALRDAHGALEVRVEERTHELALVNQALLGEIAERMETERSLRESERSLKVSQ
                     QELQQSREELRALAGRLLTAQEGERRRIARDLHDDVNQRLAMLAMDLRRIEKGDQTDP
                     AAVASMVRSITGRLTAVSDDVRQMAYRFHPSILDDLGLTKAVRRLVDDFSASTGIQAV
                     YVHHDPVSPVPTDLATCVYRIAQESLNNVARHARASEVEVELICDEGRVTLSIRDNGV
                     GFDPLQVSQMRGRLGVLSMKERVRLVRGTLDVTTAPGRGTHIEVDVPLAGNAHV"
     gene            17457..18101
                     /locus_tag="PK224_13335"
     CDS             17457..18101
                     /locus_tag="PK224_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB16772.1"
                     /translation="MSKPRVLLADDHSLVLEGFRRILDDQCDLVGMVEDGRALLEAAQ
                     RVQPDIVILDVSMPLLNGIDAATQLKKSHPSIKVIFVTMHADADYVRSAFEAGASGYL
                     LKRSAVDELEQAIRAVWAGHTYITPLIAKGLLDVLMTIGPGQSQQKKTLTFRQREVLQ
                     LLAEGRTVKDIAIRLKISTRTVEFHKAQIMEQLNLRTTAELIKYALTHGIVAPS"
     gene            18257..18502
                     /locus_tag="PK224_13340"
     CDS             18257..18502
                     /locus_tag="PK224_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16773.1"
                     /translation="MRTGDREEVGEGSPQFFGRPVLFRCRSCERVRLAGLLEMSLATW
                     QDECAMLRGQEFSREDVWWFHICCERCQPTEHTSDSV"
     gene            18567..19319
                     /locus_tag="PK224_13345"
     CDS             18567..19319
                     /locus_tag="PK224_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRB16774.1"
                     /translation="MVNVIGLGAIAGLIPVYLGILAALFLGKVLPRTWEGGLIGVANG
                     VLVYLFFDLMHEATEQTGARDVTSWAVFLGSLGVSFVGLVVLESSQVFGGRSGNRMLS
                     LPYMIAVGMGLHNLGEGLAIGASYASGEYTLSALLVAGFGLHNGTEGFGIVGAAGKTP
                     MSWRDVFLLGLIAGLPTCVGTFLSGQGVSSYFSICFYALAAGSLLYVVLSLTVMSYTA
                     TRRVQVAAGIFAGISLMYVTAMVLALVSGVRS"
     gene            19529..20632
                     /locus_tag="PK224_13350"
     CDS             19529..20632
                     /locus_tag="PK224_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRB16775.1"
                     /translation="MAGREFHDGATDGLEALEPLDPEKIHSFSELLEAMRKTAFGGRR
                     LGEAYETLAAMIEDADCKVVLTLSGAMTIAKMGKIISTMIDRGMVHAVVSTGALVAHG
                     LSESVGKLHYRHDASHSDEELFQKGYNRVYDTLEMESNLNYVEHVVSLTLKRINTEQP
                     LSSHLLTRELGKTLAEEFEGPGILKSAYLKNVPVYIPAFTDSEMGLDVGTWAMGKRMD
                     QARSQVKDGGDTAVLRALHQTCPDFNPYLDLNHYAEEVLGSKRLGIFTIGGGVPRNWA
                     QQVAPYIEISNTRLGLNVQPPRFHYGVRICPEPDYWGGLSGCTYQEGISWGKFVPPAE
                     GGRFAEVLSDATVVWPLLMVGLLERIRAKSVTA"
     gene            20629..21282
                     /locus_tag="PK224_13355"
     CDS             20629..21282
                     /locus_tag="PK224_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRB16776.1"
                     /translation="MTPVTAWLLRVGASCFLFLLVAGAALAASAPAVDHRMRGKADAP
                     VTLIEYSDFTCGYCLKFFKETWPKIQARYVDTGKVRFLYKDYPRADQGPGVTAALAAR
                     CAGDQGTYWPMHDRLFAADGRLDVDSYSQHAKAIGLDQSQFRQCLRDAPHMQAIFHDR
                     DEANSWGFHGTPGFVLMRTAQQPTTKNPAIAIPGAFPFEAFEEEIEKLLAPAGAKQK"
     gene            21355..21633
                     /locus_tag="PK224_13360"
     CDS             21355..21633
                     /locus_tag="PK224_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16777.1"
                     /translation="MASKQAFWSVPAKDGEPDLWVCMSCLSEAFCRKVPMPACPTCHG
                     VSTYEAFTLAAVQDWGTEELIAKAVAAQRDAEAISAAAPATPPLESVQ"
     gene            21662..23092
                     /locus_tag="PK224_13365"
     CDS             21662..23092
                     /locus_tag="PK224_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRB16778.1"
                     /translation="MEDGRPFLIGGRWSHTATTAPVRNPYTGETIAHVCQAGPAEAEA
                     AVQSSVEGAVAMRRLSGYARSTLLQNAAHALQSRQEEFARTMMAEAGKPLTDARREVG
                     RAIQTFSIAGEEAKRIGGEVVPLDWSPGMESYWGVTRRFPIGPILGITPFNFPLNLVV
                     HKVAPALAAGNSILIKPAPQTPLTSLLLAELLLEAGVPPGGLNVLPCDNRVAEQLVVD
                     PRFKLLSFTGSAPVGWMLKAKCGKKKVVLELGGNAAVIVEPDADLDYVAQRCVTGGFS
                     YAGQTCISVQRIFVHESIVAAFTENLVARVERLETGDPGNERTAVGPLIDPGAAQRIE
                     GWIGDAVAQGARVLSGGTRVGSVVRPTVLSQVTPAMKVSCQEVFGPLVTITPYREFDD
                     VLKAVNESEYGLQAGVFTSNIGRIFQAFEQLEVGAVLANEIPTFRADHMPYGGVKDSG
                     IGREGLRYAIEDMTEPKLLVMNLRRP"
     gene            23267..23842
                     /locus_tag="PK224_13370"
     CDS             23267..23842
                     /locus_tag="PK224_13370"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HRB16779.1"
                     /translation="MVPTHMFLTRGVGVHKEKLAAFEQALRSAGVAYCNLVSVSSILP
                     PNCKILPRKRGEKLLNPGEITFCVMARSETNERNRLISASIGLAIPTDRDTYGYLSEH
                     HAYGETDEESGEYTEDLAAQMLATTQGIEFDPDVAWKEREQVFKMGGKIVRTLNITQS
                     AVGRPNRWTCVIALAVFIPTENIPKSLRDKA"
     gene            23854..24786
                     /gene="speB"
                     /locus_tag="PK224_13375"
     CDS             23854..24786
                     /gene="speB"
                     /locus_tag="PK224_13375"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRB16780.1"
                     /translation="MALPSGWLGQADNFLGIDEPWCHPDQAGVYVLPAPYEHTSSYIL
                     GSDRGPSAIIEASQQVELYDETLRYEPYREWGGVATAATLNLDGRVDGPAVQAIQDFV
                     QPHVGRGKFLVTLTGEHTGALGAIRAHARNYPGMCVVQIDAHGDLRQAYQDNPYSHAS
                     VMARVVEDGLPLVQIGVRSISPEEIDLIQKTPRIHTFFAASILDPSGPYEGRAARWIP
                     EVLAACTGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDTLALVTALANGPGIVGMDI
                     SEIAPIEGFVAPQFSIARLIYRMLGRIRAGRRAH"
     gene            24977..25687
                     /locus_tag="PK224_13380"
     CDS             24977..25687
                     /locus_tag="PK224_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB16781.1"
                     /translation="MRINKFFTEQGLCSRREADRLIAEGRVTINGQVAKLGDQVSPQD
                     LVARDGVVLQRGNRSVYLKYHKPVGVTTTTEPHVRRNIIAEIGHTARIFPIGRLDKDS
                     SGLILLTNDGDIVNEILRVEHGHEREYRVEVDREFDETFLVRMAQGVTILGVPTRPCI
                     VARLGPRRFRIILTEGRNRQIRRMCQALGYRVVSLHRVRIMHITIEGLHAGQWMDLTH
                     DERSRLFQALGRSADSSM"
     gene            complement(25732..26490)
                     /locus_tag="PK224_13385"
     CDS             complement(25732..26490)
                     /locus_tag="PK224_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HRB16782.1"
                     /translation="MSLASRLKLSFPTTNQLIISLLIAGLGWVSGQALSRIDQDLRIM
                     YTEYTLGAADLAHISADVIRYRNTIIRSLEAENQTTFERITESLPTQRARIQHAVDRY
                     AAAGLRVSRSGRSEEKDIQAVRESLDQYFHVASKTIDMLSQEWSAGSATEAAELRRKA
                     EIHAADNGGPKVMQVSLALDRLLETVAEVAKDMRDEGTKTIVTTGYWIVGGSFLIALL
                     NLFLTRPVRIQELPSSRPEATPHPGSPRSLPSEG"
     gene            complement(26591..28267)
                     /locus_tag="PK224_13390"
     CDS             complement(26591..28267)
                     /locus_tag="PK224_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB16783.1"
                     /translation="MNYTATETPISTPSAWTILARLNLLIGLERRILTIVVSYAVVIG
                     LFALIVPLTVQELVNTFAFAIQPIMIVTLVATMFGALLAMGAFRVLQARAVEILVQRL
                     YTRLAVAFTEALPRFRENVFLPQHTNTFIEAELLPRALVAMLVDVINVSVSGAIGMAI
                     LIMYHPYFLGYNTLLITGFAFLLTFFGRGGLRITQRVSRLHYQTFHWLQDIGINRLHF
                     KSTDSLPLLLKKTDALVKAYVMARKTRSDILSGAQYKSTVIFQAVAHSGMIGLGGWLL
                     SVGDITLGQFVAAEVIVGTLLLNLDTVARRMYAAIYVATSLHELSTLFDMPKEDVSGP
                     VAAWLPDPSLQGVRLTCKDVSFAAPDGPLLFERFNLEVMPGEKISVLSGTSRSKTSLA
                     LVLAGLYHPTGGVIRYNDIDLRDVSLNYVNRCRGLMLDSHPTLFDGSLEENITLQRPS
                     IQFEDLSWALRFVELEEEIDAMPQGLETLVHGNGANLTRSQVLRVLLARMIVTRPELL
                     IFNGSLHNIEPALRLTLLRRLCSKEEPWSVVFVSNDPEVSQLVERRVVLD"
     gene            complement(28417..30084)
                     /locus_tag="PK224_13395"
     CDS             complement(28417..30084)
                     /locus_tag="PK224_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRB16784.1"
                     /translation="MAQDHSDNQSNLFQAVIGHLGLLFRLERRVLGLIVSYSIAIGLF
                     SLIVPLTVQELVNTFAFALQPITIVTLAAVMVAALLFVGAFRALQYYAVEVLERRIFA
                     RVAIAMAQQLPHLRYQGFKPRFANFFVETILMQRAVSVLLVDLINVIVGGAVGMTILV
                     FYHPYFLLYNAILLVGFNVVFFLMSHGGLKADMNLSHAKYDTLHWFQEIAYNLLHFKS
                     TDSQALLVKKTDQLLDTYIGIRRTRFGILIRQYLGSLGWQAIAHSGLIATAGWLLGIG
                     QLTLGQFVAAEVVVSGILSSFDGVVKRMGHIYYFLTALTELSFLFSLPKDQDTATLSI
                     PLPDPTVHGIRVTCKDLTFAPAGGPAVFERFNLEVTPGEKIGIYADSTMAKTALARVL
                     GGLESPTSGVIRYNGVDLRHLNLDSVNRFRGFILDSQLSLFEGTLEENIVLGREYIPY
                     SDVRWALRFTELEEEVDALPHGLKTHVRAAGKIFAPTHIVRILVARAILGRPQLLIFE
                     GILHNMHPAMRETILRRLCSKEEPWSVIFISNDPNLTPHVDRRIMLN"
     gene            complement(30219..31706)
                     /locus_tag="PK224_13400"
     CDS             complement(30219..31706)
                     /locus_tag="PK224_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB16785.1"
                     /translation="MKIHAWVLALAACGLCSLPDTGLSADETAKPRTLPPIPLSLNEV
                     HAWIDRSHPLLKGAGTEKTSARGKMLKALGAFEPVLVNDTEIERFIDKGTTKTQSVGF
                     NDTLVEARTPWGFRGSAGVRQSIGDAKIPDLGFGNGQQVILGGFLPLLKGLMINPENA
                     ELQRSELADPRADVKISQTRQDLFLAAATQFWDWVSAAKYVDVQRRALGVAEERLRQV
                     EGRAKAGAVAPLDVVEAGQEVQRRREIAIGAQRAVEQEQYKMSMFLWENQTPTAPQVE
                     RAPDFPPPTAVPTPEIVRADKLQAKTDRPEIREVDIEAKVNNIDLELAKNNLLPSLDL
                     EAAPARAPEKFVLGLGYRFGVELRMPILQRRSRGEVMEAQAQADRLVMVQKYREQQVV
                     VDVDNALSAIERAKERVTAAAESLRMAKTLEEGERFRFSLGATSVLFVNLRERNSVDS
                     EMQLVRAKADYQKALALYQWATGIWARSQPSATPVNYRVGASFSK"
     gene            complement(31796..33232)
                     /locus_tag="PK224_13405"
     CDS             complement(31796..33232)
                     /locus_tag="PK224_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRB16786.1"
                     /translation="MQRIREGLKSRIRSLVTQRTGLDEMAVDDLAISTQLQSWVAVQI
                     PERLRFSSRLAILLVGFFILIVAFVPWTQTITVTGQLSAYTPFERPQDVEAQITGRIR
                     KWHVFEGVRVKAGDVILELDDYDPNFMAPDLLSLLDQRKKALEDTRKAALSRADQLDK
                     RIKEMQNLVKAAVPSAGARVLEAESKVREARQKIEASKIAVATAELNVERHQQLAQQG
                     LVSQRELELTIQSALASKADLQGAQANLSAAEQSMKALSFGREQVSAEVLQRLLDAEA
                     ARDASIGEAARAADQVADVSLRLSNANQRRVAGRILSPIDGTVVKMAQAGAGETVRPG
                     DKLVRISPSSTDKAIEMVADGIDAPLLNVGRKVKILFYGIPAIPLPAWPELMAGTYNG
                     VVKVIDQVDDGKGNFRFWVVPDLEERPWPPQEHVRQGTKAMGWVILNRVPLWYELWRR
                     FNLFPPDYQERPPSLIDTLLPKAGRGAK"
     gene            33409..33963
                     /gene="purE"
                     /locus_tag="PK224_13410"
     CDS             33409..33963
                     /gene="purE"
                     /locus_tag="PK224_13410"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRB16787.1"
                     /translation="MSKNNTAVRASVGVLGGSKSDFPILEKAVAMLNELGIPNELLVV
                     SAHRTPDRLFAYAEQAIDRGIQVIIAGAGGAAHLPGMLAAKTSLPVIGVPIPTENLRG
                     LDSLLSIVQMPRGIPVATVAIGGAENAAILAAQILGLRSAAIRQRVEQFRANQTQSVL
                     DSQDDARLSPPLRMGRSSRASRRS"
     gene            33960..35126
                     /locus_tag="PK224_13415"
     CDS             33960..35126
                     /locus_tag="PK224_13415"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRB16788.1"
                     /translation="MNVTVIDPGATLGVLGGGQLGAMFATAARRLGYAVVVWDPDPEA
                     PAHRLADSSLIRSFTDSAARQDFIRRVCAVTYEWENVPADLCEQLERDVPVRPSSRVL
                     RVIQDRIEQKTFLRTHGLPVPAFHAISSPEELSRQRALEYPLVCKTATAGYDGKGQWK
                     IARAEDCAAVEEDLARSLRPGSRWILEAWLPFERELSILVVRGMDGAVRAYPLVENVH
                     EEGILRQSTVPADVPATVADQATVMARSAVEALDGVGVFCVELFLMPGGRLLINEVAP
                     RPHNSGHYSLDACTVSQFEQQVRALCGLPLGEVRLLSPTVMLNLIGDEVLLATVSPAA
                     QDLLREPGAVVHLYGKRVVRPKRKMGHISFLAATREAALSNASVFRSRLARYHS"
     gene            35374..37236
                     /locus_tag="PK224_13420"
     CDS             35374..37236
                     /locus_tag="PK224_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16789.1"
                     /translation="MSVPLSSSDLDQTGRHPNIRGLGRLLDHSRTRTIFLQSLVAGIL
                     SYELLVSNETIFGHRVSLMVAAGLILVSCGIMLLPRNLLESAWFPGALISINTLFVTG
                     TIYLSGTASSELYLTYFLLLLIASSAPSLRQLLGLSTVICAGYGVLVYEHAMQSGGLE
                     VGHLLGIPVLLIMSVFYGLTLETVATERRRNRLLRENVQGLKQSEQVLEERRTQLETR
                     VQGLKQGLSRANQEIRQGMVERTGLERQLREAQKFEAVGRLASKLANEFNQVLAVIGS
                     QTGVIVSKLKPDDPLQRPVEEIFRSGERAATLTAQLLALRVHETAIRDTLSLSPAIES
                     MRDTFEALLPGRIDLRLANESIPVLVEIGHDRLEQLLLHLVANARDAMPKTGRVEIAL
                     EIVTGELLPAEQLEKNPRKRMARIAVSDNGGGMNLDVQSQMFEPFFSTKDTHAGLGLT
                     LVYGIVRQYGGTVEVQSQPGQGTTVRVYFPLVERNGVVRDTKVLSGALSKGAETVLLV
                     EEDEITRKLASSTLQNHRYQVLEAGSAVEALLVAQQHAGPIHLTVSHLSMPEISGREL
                     AKRLVLQHPKMKALFVSGFSDDTIASHRVNKKYFLQQPYRQHDLAGKIRELLDV"
     gene            complement(37340..38044)
                     /locus_tag="PK224_13425"
     CDS             complement(37340..38044)
                     /locus_tag="PK224_13425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRB16790.1"
                     /translation="MLPLNDDNPTEHTPVVTITFIVACCLVFLYQSSLPPAPGEAFVY
                     QYGAIPSVVFGHAMLPPEVTAVPAFATMLTSMFLHGSWMHLIGNMLYLWVFGNNIEDI
                     MGHARFILFYVVCGVLAALSHALTDPTSTVPMVGASGAISGVLGAYLLLFPHARVLLL
                     APIVGTTYVPAGIVLGFWFVMQLLNGGASIGSQGGGVAFFAHIGGFITGMILIGFFKR
                     PEVRFFTPGRTRSWRY"
     gene            complement(38069..38923)
                     /locus_tag="PK224_13430"
     CDS             complement(38069..38923)
                     /locus_tag="PK224_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRB16791.1"
                     /translation="MSAAVMRSRAASPPTLILYHAECADGFGAAWAIWRRYPNAEYRP
                     VKHGEGPPANLAGHHIVIVDFSYARPTLEAIAKDAASLVVLDHHITAEQTLADLPYAY
                     FDQKKSGAVLGWEWAHDEPAPWLLRYIQDKDLWNWALPNSREISAALASYAFDFQLWT
                     SFEQQELEREGRAILRYENELVTKLASHAALVQFEGATVPAVHSAVLTSQIGERLSAA
                     HPFCLIWHDRNGRRYYSMRSREEGTDVGSIAASFGGGGHTHAAGFSIPMQADGSLPSN
                     PRLPRPAP"
     gene            complement(39024..40079)
                     /locus_tag="PK224_13435"
     CDS             complement(39024..40079)
                     /locus_tag="PK224_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRB16792.1"
                     /translation="MKSPAARSKPTPTKEEILAQITTLLDRPDDDIQAALMKEILAGV
                     IRLSESQLDVLDLKIVNRALKELRHAFRVFQGYRQRLKISVFGSARTPADDPNYQLAF
                     QFARRMVEEGYMTITGGADGIMRASQEGAGREHSFGVNIMLPFEQGANAVIADDPKLV
                     TFKYFFTRKLMFQKESHAIALFPGGFGTHDEGFEIMTLVQTGKSDPKPIVCLQAPGCD
                     YWDHWNSFITDQLLKRRLINAEDLALFTIVDNVEDAVTEIRTFYRRYHSLRFVGRQLA
                     IRLKTPLSAAQVEEVQQQFSDMLTEGTFEQRPSLPEEIDEPGLKDLARLTFSFNRRNA
                     GRLRQLINHLNQLPSTA"
     gene            complement(40190..40900)
                     /locus_tag="PK224_13440"
     CDS             complement(40190..40900)
                     /locus_tag="PK224_13440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB16793.1"
                     /translation="MMTPGVVQVPGDVSVSEAALLLDRERIPCLLVKDSETTFGIMTS
                     ADIVMKVVAQGLEPHDVEVRTIMSKPVHSIECDQILDDATSVMASTGASLLIVTKQGQ
                     PVGILTARDLALAPKRCETCLPASIRVTNGEGEGAKHLATIRQLSHVGAFIESRTLLL
                     PGTSIVLSFMLPGTDLSFSLRGTILNSSYEPEFHEDRDALGTYPGVDIQFAPLPSSDE
                     SKIRAWVLQNLPRASGLS"
     gene            41128..>41839
                     /locus_tag="PK224_13445"
     CDS             41128..>41839
                     /locus_tag="PK224_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16794.1"
                     /translation="MVASKYAKRDLMQSEFTERAIGLATHGVGLSVDVYSPDLLHLVH
                     SLRDAGLQPGYLEVFKATTSAMQWARRHLPDMKLPYHGEGLWVTQPDFPRSSSGTQGV
                     AEACAQILALGSAWLNHECAMKQMVGYSFGTYLPPLYTELSARMTAENLAFLQEQVDE
                     QARRHGTDPALVLLEMPPLTYFGCGSLAIPAFFRAVTDRVACGLVLDIGHLWTVYRYT
                     GAWLRQTLEAFAAEFLDAF"
BASE COUNT         8565 a        11531 c        12673 g         9070 t
ORIGIN      
        1 ttcgtgaagc caggctacca cttatcacac tgtccgaatt tgcggagccc cctctgagag
       61 cggcagttcc ttcggcaccc ctttgagcct gcttcctggt gccggaatca accactcgcc
      121 gtcaccccgt tgaatcagcc actcgtgatg aagggcgata atcttcgcct cacgcaacgt
      181 cgtcagcagt gccagcatca tcatccgttg aatcgacggc gctgccgcgt gatagatgcg
      241 gaacagctcc caggcctcgg ccacccgctc acgcttcgcc cctgatggca catccaacga
      301 gagcagccgg ttcttatcga gatcctcgtt cgccacggca taattgagca acccgagcaa
      361 gacgccgcat tcccgcttga tcgtgccgtc tgccgcgtcc tgaccgcgac gatgggcgat
      421 gtagccgacg ccgtcttgca gcttcagcga gctcaaggga cggacgccga agaacggcac
      481 cagatggcga tgcagaatcg tgctcgggcg atccagtgcc cgcaggccct tgctctgcag
      541 tttcgcgaga tatttcggtg tgaactcggc aaacgtgggg ccctgatccc cggcaacgcc
      601 ccctacatgt gattggatcg aggccaggat ctgcaccatc gccgcatgtt cctgatcgct
      661 catgaggtca tatcccaaca ccggccggta gcgcaccccg cgataccgga aatccaggtg
      721 ggaacgcatc ccctgtgccg tcatggtacg gttgattttc attggggccc ctccagtgcc
      781 cccaaccatt ccccagggca atcccgcacc ttgcgcgtgc ggcaaaacgt ggcgggctcg
      841 tgcaccgcgc acagttccgt gtagaagcac acctcgctcc caaagaccca ggctggcatc
      901 gggcgattga ggttcaggcc ctcctctcga taccagtacc agccagactt cgtcggtggc
      961 tccatgctcc gcttcatcgc gtccccctaa tcgcaaagag cgggtttcgg tgctgcacga
     1021 tgcgtccgct cgtcgtctgg gcagacaaag tccccttaat cacgagtgac gcgggtgggc
     1081 ggcttggcgc tctgtcctgt cctccctgtt ttcggctcgc cacataggcc tccagatcgc
     1141 tcaggtcgta ccggagctgg cctcccacat acgccctcgg gatagggagc cgacgcacgg
     1201 tacgtgggct cactctcagc agttccgcag tttcgatcag cgtcagcagt gtcatcttgc
     1261 tctccttgcc ggccgatccg ccgggttgct tgcagcggtg aactactcct ctgggtttct
     1321 tgtaagactg gtcttattga gcggtggttg gagtgcggcg atccactgcg cctcaacgcg
     1381 aagcagttcg gattccggga cgggtatgaa caagacgcgc tcccatcgtt ttccgctctg
     1441 ggaatgctga gccagtcgtg atgggagatg ccgggattgg ccgacatacg ccacggcgcc
     1501 ggcttggatc aagaaataca cacagggcgg aatgtcgagg gccgggcaat cgcagagccg
     1561 gtcctggact gctgccaatg ccactggaac aggctgggtg agcggcctct gaacgatcgg
     1621 tcggaggttc agtggtaggg gcgcaccttc gcacattacc gttagatgct gccgaacgta
     1681 tcgcttgagc gtggtcgggc agaacagcgg ttctccgcca tcgatccgga agtgggggag
     1741 gagcatggct tcggcgagct cgcgaattct gccaacagac agccggactt gttccgcgac
     1801 ttcctgcgct gttttgagac tggtcagaga aggaccctcg accgcgccgc tgatgttctg
     1861 caacgccccc aaagttccca ttcgacgcac tcctttgtgc tggttatgag cgattctgct
     1921 cgtctcgttt ctgaaactct cgctccagca agctgcgaat gaacccgctg gaggtcgtgc
     1981 cttgttttct caaggcgtcg agctttgcct taatcgcctg ggggatctga atcaggagtt
     2041 tcaccatggc catagaggag ttcctacctt tcgccataaa tatagatctc aatatatcta
     2101 tatgtcaatg catattgttg actaggttta ggagcacagt cacaatgccg cccgaggtgc
     2161 atgatgaaga gccaggaaaa ggcagaaaaa ccgcggaaga cctttgggtt gagcttgaat
     2221 cgagagctga tgctggaaat tcagcacctc gccttggatc aggacaggta cgtgaatgag
     2281 ttgttggaag aggctgcgaa ggatctactg aagaagtaca aagaaaagcg gcattgaatt
     2341 taatgagagg agatacagat gacctctaat gaactgaaag agcacattcg tcttatgata
     2401 cagcctgtcg atctcgaggg gcttatcgct caaggcgtac ttgcgaaggc gggcgcctgg
     2461 tatcgaatta tgaacatgaa gaagctaccc aagagcgttt caatgcatat aagtgaaata
     2521 tctcgagatt cacaggggcg agccaagata aagtttgcaa aagtaagcga tcgtcaagca
     2581 gtgagtcttc gcaaattcct ttaagcaggt caggaggaac agtgcgactg gcacgatgat
     2641 cccgacctac aaatcaatat ttaagaccct gcaaagctat gcagactctc acagatgaag
     2701 tatttagacg actgctgatt gccaaaggct tgctcggcaa gattcgattt tcgaattcgg
     2761 cgcagccaga tcgggtaatg ttggctagcc atattcttgc tgcccatgac gcagctgaat
     2821 tggcggctgc agcgattgca catcaccttc aagccggcgc tgataaggga aaaaccttcc
     2881 taatggatta cctgccgttg attgaaaaga aggcccatga gggtaggcaa atcgagggaa
     2941 gagattattt ctcgcaatta aatgacgtgc gagttgcgct caagcacaaa gggatactcc
     3001 cggacgcaaa agactggaga cgggttggcg aacggacttg gggttatata tcggtctggt
     3061 gtgacgaata ctttggtttg tcgctcgatg gtctcgatga atcagggctt atttcagatc
     3121 caaatgtgaa ggcgcattac actaccgcta gagcaacttt tgaaaagggc ctgtacaaag
     3181 agtgttttga acatcttgct ctcgcatgtc aaaagttgtt tgatgaaaat gatgccctgc
     3241 gcaacttgtc cgcgggtgaa gcaaaggctg aggacgctat aaagcttgct gcctatgggg
     3301 tacatgccaa tgactatctc gctctacagc agttcttgcc gaaggtaatt ataagttccc
     3361 aggatccccc ggcatgtcac tgggagcaaa atctatacgg tcatcccgca aattggaccg
     3421 aaaggaacgc agaatttggc ctccgagcct ttgttgatct tactcttcga attcaagacg
     3481 ctgattggat tccaggtccc tacgctttcc atctgatcta tgattacaaa attacggcaa
     3541 tcgatgaaac cgttgctgta gttcgtgaac gaactccacg ctctcttcta gatgctgtta
     3601 ttgaggataa ggatgaggtt cgaatcctaa agaagggaaa ttcgattcgc tgtcgtgtca
     3661 tcgtgcaaaa tccgatctcc ctcaaactgt ccgatttcgc tccagatacc ctaataattg
     3721 tcgacgatga aaatattacg catcggttga aacgcagcgg ggtccaaata acttgtgttc
     3781 cgagagaaga tccaattatt gggaaatatt tcccggacct aagtgagata ccctacgaat
     3841 cgtgaatggg agagtttgaa ggcggatatt aggcatcgct ccagaggtgt aatttcgact
     3901 cagcccatgc tgggcactct taagtgtctg atcgcccaga atagcctggt gttttaatgc
     3961 caaatatcgc attcctcagt ctcatctacc tcctgctcgc ggtgcctggt ttccttgttc
     4021 gatcggccta cttttcggga gagttcacgc gaaatcttct gccgagaagc tggaccgacg
     4081 agatcggtaa agccatactt tattccgttc ctttccatct cctgtgggtc ggccttttcg
     4141 agctgacaca atgtcttggc attacccatc acaccttgac cacggagact gtgctccggc
     4201 tcatggctgc tgaatatacc tcccatgact atagcttcca tggaatcgtt tcacggctcg
     4261 ccgagaacaa gacctacctc ttcgtctact attccggagt ggttctgacg tccatcggcg
     4321 ctggatggat cgctcgaagg attgtctgga gggacgaact ggatgtgaaa tggccctggc
     4381 ttcgctacag aagcgaatgg ctttataaga tcatggggcg aggtggattg aaggatgttc
     4441 cctggaaaga cacggaatcc tggattgata ttctttcgga gcaggacgcc ctagagcctg
     4501 gaaaagcggt cctatatcgt ggcttggctg cagggtatac gacggaagat aatggagcac
     4561 ttcgagatgt tattttgacg gatgtgaagc gaacgatagg gaagaagaat tcgaatggtg
     4621 acgtcgtatg gacccacgtc ggtggcaggt ttttcgtgat gagttatagc aaggtcagaa
     4681 acatgaacat tacttatgag caatattcgc gtaagttgct agagcagatt tcagatcttc
     4741 cagccggacc agtgccgttt gggcctgagg aggaggggga gagtccttca tcatctcagt
     4801 aacgcgttct tctagtctat cgagatattt gagcaccttg gacgcttctt ctggagttgc
     4861 acggcctttc tggacgttca gcagaacctc ttttatcttg cctaggtctt gatagtcttc
     4921 accccgctca gctgcttttt ccatcagtgc gaagtatgta cccatgggtc accttataac
     4981 ttggtcgaaa atcagcgaac cagaagaaag atttgagccc gtcctgtacc accatggtgg
     5041 taccaagctg atactactac aacgtatgac ataaggccac aaccggtgac aaatgatgac
     5101 atctatgccc atcatattcc gcgatgaagt ggcttcggag ctccgaatat ttacaattac
     5161 cgggctgtcc tatatcggac agcctatctg taagccattg taaaagctcg ctactcatcg
     5221 gactcttcag aggaatcatc ttgttgtttt tcctcagttt tgtccgtcct ctcaaggctc
     5281 agacacgggt tcaaatcccg ttgggaccac caaccttttc acacacttag caccaaccgt
     5341 ggtcggtcac acccggccat tgtgctgaat ttgtgatagt catccttaaa cacctgcacg
     5401 gcttcccgca aattctctgg cgccagatgc gcatagcgct gcgtcatccg tccatcgcga
     5461 tgcccgagca accgttgcac ccgatagaga tcaacccctc gctgcacaag tgcggaggcg
     5521 aaggtatgcc gcaggtcgtg gaagcgaaag ttcgtcacgc ctacggactc acaggctcgc
     5581 cgaaaggccg tcgtgaccat atccggcgtg accgcttctc tgtctggagt gtgttgcaga
     5641 aacaccgacc cgctcgctaa cggtcggcag ggtttcagga cttccaaggt ttgccttgca
     5701 ggatcgcaca ggggaatgcc caagcgatcg ccgttcttgg tctgttccag aataatgaga
     5761 ccccgaacca aatcgacttg ctcccactgc aagcacacca cgttttcccg gcgcagcccg
     5821 gtgtaccgtg ccagcatcac gatcggtcgc aaccacatcg gacaggcctg gtaaattttc
     5881 gctagcgtct cgtcatcgca gtaccggacg cgtgcattgt ttaccttgcc catcgacacc
     5941 cgacacaccg gattatctcg acaccattcc cattctcgaa tggcgacgtt gaaggcggtc
     6001 ttcatcaacg ccaattcctt cacgatggtg gccggcgctg ctcccgcctg ttgccgtttc
     6061 cacttgtacg cggcaatctc cttcggtgtg acccgaacca aggtcagttt gccaaacgtc
     6121 ggcagcaggt gagccaagat gccccgcgct cgccgttcac cgtgacggct ggctcccacg
     6181 actcgctcag ccacataacg ctcgaccatt tcttcaaagg tccgttcttg ttcttcgagt
     6241 cggtcgaaat agcgaccttc gaccagtttg accttgatct ttccgagcac cgcttctgcg
     6301 agtcggcggt ctgtacattc agtactccga cggatctgct gtccctgata cacaaacgtc
     6361 atccaccata ctttattccg cttaaacagc cccatctgca tcctccttct ggatgaggcc
     6421 tgatttcggt ctagtttccc cgtggcgggg agtatagacc tcggatttgg cggcttcaat
     6481 gaggtggttc agatcgcggg aatcgtcgtg aatgggcagt gggaaaggct tgacgggaat
     6541 tgccccaaag cccttcagcc acgcctggat ggcgtcaggg tcgaaccgga tgaggccatg
     6601 aatgcgatgg caaggaatct tgttctgtga gacccagaga taaagcgtgg attgtttgat
     6661 attgagccac gcggagagtt ctttgacggt aagcatgatg cagatacacc gggggagtct
     6721 cgcgacaccc ccagtccccc taacatgggc tgcccgcgtt cccgccggca gacgacgtgt
     6781 gaaaccgagt gggtggttgc ttgagcaagt tccggtgccg gtctttccac ctggagatgc
     6841 ccctgactat ttcgtgctgt agccattctt ccccaccagg ggtggcacaa atgaccgcca
     6901 acattggcgt cagggtgtcg ctcacccatc gtttcacgtt ctgcagggtc tgcacctgct
     6961 tctcctgggc taatcgccct ttttgaaatc cctccgtcag gagggcgtac cactccagca
     7021 ctggagcccg gtatcgttct tcatcctcgg catccttcgg aatctgccgg aaatccacat
     7081 aggatcgaag caatccaacc accaattctt tccaatcggc ttcgtctagc gaggctaagg
     7141 ctcgggcaca ttgttcggcg cgatccttct tcagttccaa ttcccacctg gtaccatact
     7201 cctcccaatt ctcctgttcc ttactcttga gttcgagacg cttgtcgtaa atgcgaagta
     7261 gggtctgact ctgcggactg ccaaaatata gggtctcgcc cgtcgtctct ccgttcccgt
     7321 gggtcagatt ggaaacaatg tgtcggacct gtgctgcacg agtcacgcac tgcccggcag
     7381 cgacggcctc tcgaatcgcc gagactgaga cggtcgcccc tcgatcatcg agggcacaat
     7441 caatccgagt cacatgcccc tgctgggcat ggacccactt caggagcact cgaatctgat
     7501 caagcgtcag cgcggagacc aaaccaccag agagatcgag atgaatttca ttcggacgac
     7561 gaggcgcgtt cgtgcccagt ttgcccactc ctcgcagtcc atctgtcttg atccacgaga
     7621 ggggataccc tcgaaacccc cctttggctc gattccactc tccaccgagg accttcatgg
     7681 tctcttgaga attgctggcc aggaccgtaa aggccaacca atcaatggag agagtgaatg
     7741 tagaatccat ggaatctatc gacctcctgt aaggagcgct gtaggtagcg cccccgtgtt
     7801 accaagacgg gggccattcc ggtccgcccc gcagcctgcc ggcatgcggc gcggaccggc
     7861 tttgcctccc ggccacggac gacggtgttg ctctaaacag gactccattc ggagtgtctt
     7921 cgcgtgcttg tgatcgttcc ggtgcatgct tagaccttat gcaaacgcca ccacgcagta
     7981 aagaaccctc tagaggacag gaggggtcac gaggggacaa atcgaggaca gattatcgaa
     8041 cggcagttgg gggaaaggaa gtgagcataa ggaggcgtat gtgtgctttg ctgcgctagt
     8101 ggcggcaatt gtgcacatcg ttcggagccg ggacatcgag caaacagaaa tcggctcgtt
     8161 tttgcatggt gcgcgaaaaa aagtttgagc aggaattcga agccttgtgg ggtacgatca
     8221 aggacgggtt ctcctatgac agccatgcca atctcgcgag acctcctgga atgccaggaa
     8281 cagactggct gaaatgaatt gcatcagctt gacggagact agttggacaa atcacacgcg
     8341 aagcactctt attcacgagt gagaaggtaa agaaaagcag gatgaatagg atgatcgcac
     8401 gacttatctt tctccttgta tgggctagcc aaggtctcct ggctttatct caagtccacg
     8461 cagagcagat ggagccaggc gaatttgctc agggcgttga gtacaagttg ttgatgagca
     8521 agtacgagat ggtgtgcaaa cacatgctca cgctgttcaa tcaggatctc gccaagtttg
     8581 gaactgaaaa atacgacgaa catgaagagt ttcgctcgat tgggtagaaa aagaaacgat
     8641 cacccgtatg gatggcgacc gagaagttac ggattttgtc gaggtcgcgc acttcgatat
     8701 cgataatgac gggaagatag atctcgtttt caggctaaga ggccccatga ggggatacga
     8761 tagagataca ctctatattt tcccaggcct cggactgagt gagaaacgat ggacactgat
     8821 ggaaattgcc catagcccag gcaggatttc tcatgatggc tacttcttgc tcactcagag
     8881 tggtgcagag aagaaaaaaa ggagaggggc taggcgagta tttctcgact ggagcctttt
     8941 gtgtacgaag gaactgtcta tgttgggatg cgacccttgt atgaactcgc cagcgggact
     9001 gatccggtta cagagttttc aaaggtggtg gtgattacaa aatatcttga agggaaatat
     9061 ggcggtggcc cagatgatcc gaacgaaaag gagacgggca agagagacga cgtgtgctat
     9121 ttgaaaatac gaacaaagcc atacgttcct ggaggtcagc gatgagatgt gaaagctaga
     9181 acttaatctg acagtccggt agagtcctcg ccttcacgga aggagtgagg accatgacga
     9241 cggaacagaa gatcatcaaa accaaggtgg gcgtgttgga attggcgaaa cacctgggca
     9301 atgtctccaa ggcctgccag gtgatgggct atagccggga tagcttctac cgtttcaagg
     9361 aactctacga cacgggcggc gaagccgcgc tgcaggagat ctcccggcag aagccgctcc
     9421 tgaagaatcg ggtcgctccc gaggtggagc aggccatcgt gcacatggcg attgagcaac
     9481 ccgcctgggg ccaactacgc gtggcgaacg aactccgcaa acgggccctg acgatctccc
     9541 ccgcaggcgt ccgatgcatt tggctccggc atgatctgga gaccatgcgg aaacggctca
     9601 aggccttgga ggccaaggtc gctcaagagg gcctcgtctt aacggaggcg caggtggtgg
     9661 ccttggagaa ggcgaaggcc gacaaagaag cgcatggcga gtttgagagt gaatgccccg
     9721 gatattgtgg tgcgcaggac accttctacg tcggaacgct caagggcgtc gggcggatct
     9781 atcagcagac cttcatcgat acctacagca aagtcggatt tgccaagctg tatgaccgga
     9841 aaacgccgct gactgccgcc gagttgctga atgaccgagt gctgccgttc ttcgagcatc
     9901 acgaaatccc cctgaatcga gtgctcacgg atcgcggcac ggagtactgc ggggcacccg
     9961 aacgacacga gtatgaactg tatctcgcgg tcgagaacat tgatcatacc cgtacgaaga
    10021 cccggcatcc ccagacgaac gggatctgcg agcgattcca caagaccatg ctgaacgagt
    10081 tgtatcgcgt ggccttccgg aagaagatct atcggaccct ggaggagctg cagactgatc
    10141 tcgacgcgtg gttggcggac tacaacaacc aacggcccca tcaaggccgg tggtgttttg
    10201 ggaagacacc gatgcagaca tttattgata gtgtgccgtt ggcgaaggaa aaaatcttgg
    10261 cggcctaacc cggtgaagac tcgctggctg tcagatgaag tcttgagcta tacagatgag
    10321 aaccggattg cacacaacct attcgcaagg ctcttacgcg ctgtgttgat gactttgaac
    10381 aagttaactg gcatagctgg ttcaaagcta gccgatttgt cggaaaacta tgaagcacga
    10441 gccgattacc aggtagcctt tttcttctac agctttgcag cattgttatt attcttattt
    10501 cttgctgcag ccattctctt tatcatgcag aaaattgttt cgatcaaaag tcggaattag
    10561 tagcgggtag acaggcgcga gtgttcattc agatgacaga tgcttagtgc gctaaaaaag
    10621 cttggaaggc attccggatt tctggggata tgatcagagc caggtggacc tatgcgctcc
    10681 gtaattagga caacagggac aattgcgatt ctgtggatct tgtctatggg cacatcggtc
    10741 ggctcttccc ctgtgtcgca ggatggacgt aagtggacga gtaacgatct gaatgggcca
    10801 gttcatgtaa tgcggcaaca gacaacgaac ttccgtcaag ttggggacga gtggatagag
    10861 gaagagcctc gtgatgaata cgtgtcaaca tatgatcgga caaaattggt tctagaaaag
    10921 gaatggcttc aaccgattct gcccccgctt ccacaaaaaa ccttcacatg cttcggtcgt
    10981 gatccgatgg ggaagattgt gcaggcgttc cagtgcgatg caagccattc tcccatcgac
    11041 gggtttaggg aagtgttcct atatgacagc aagggatatc tcgccgagca gcagatgatc
    11101 tacctcgacg aaatcccgtc gcgcgccagt cgcgaggtat accagtatga cgagacaggg
    11161 ctgaaagttt ccgagcggga atacgatgcc catggcgccc tcacacgagt tgtcactcta
    11221 gaacgagatc cgactaagca cgctctctcc gtctccgctc ggactgggga tgggatgctt
    11281 tggtccaaat cccgtcacat ctgtgacgat aaaggacgtc caattgaaag cgtaaatctt
    11341 gatcccgatg accaaattct ttcaacccat cgcatgacgt atgacagtta tggaaattgg
    11401 attgaacgga cgagttccgg acaagggctc gatcttcgag aggtggtcgt ctatgagtat
    11461 gatcgctggg ggaattggac caaaaagacg agccgcgtaa taggaacggg aggcgaaagt
    11521 cgatggacca tccgccgaac catcgagtat tttcccgatt gatggcatgc gatgttctgg
    11581 tttcccatgc tttgtgagga aaccaatgca acagtggagc aattaagaaa tctaactttt
    11641 aaccttcaga aacggatgaa attcttgcct gttctcttcg cactagcctt tttgtccttg
    11701 gagtcgtgct tgtgtacggc acctatagta ggtggcctgg tttagtagat cctcccgata
    11761 aatgtgctct attttactct caggctttgc taaagaagtg gtttggtcgc atctttgtaa
    11821 tcgcgtatac ctacatcctt ggtctgctgt ttattgtatt tgcatgcata ggactctgga
    11881 acggattcac gaaatgactc cacctatccc ggcagtctag caggtcaaag gtcaggtcgt
    11941 cgtgaggcag cggtctcgcc ctggctagtc ggagcgtttc cagctttcga tgctgagacc
    12001 agcgatgcgc tggaagtgcg cgaggttgtt cgtaatcaac ggacgctgcg cgtccaaggc
    12061 ggtagcggca atgagcaggt ctgcatcggg cagcaaggcc ccctgtcgat ggagatcgcc
    12121 gtataacgtc gccgcgcgct ctacgacttg gtcggtaatc gggagaatga gactggcttg
    12181 gcagagcaac gtgaaggccg cctcttgagt ccgcgcctgt ttagccttca gcccgcgcag
    12241 gagttcgtag cgggtgatga tggagaaggc gagccgttcg tgttcggcga gataggtccg
    12301 cacccgttgc aggacgagcg gatgttgttt gaggagttca gagagaatat cggtgtcgag
    12361 taggactgcg ggttgaggca ttacgcggcc ggttcacgaa agaactgctg gcggttcaag
    12421 gcgatggtct ctacctgtgc aatgtcgtct gcagtcagcc cctggtagac cagtgcggca
    12481 cgtcgcaaaa tctcctcggc attgaccgcc atctcggtgt ccaccgtgac ctgcacttct
    12541 gcatcgtccg tgagcgctaa gggctccaac ggttgtaaga ctccgtcatg gtatcgggct
    12601 ttgatcgttt gtcgcatcgt gaagcctccc ccccgctctc tccctgtcca cttcacttga
    12661 gccgtctcgg tcgatcccat tctacccaaa ccgccctaaa atctcactac ttgtttacag
    12721 aaccatccct gtcgcgcggt atgcacaagg gcaatccgtt caccatcacc ggtgcgcgag
    12781 caggcgggca aactcggcgg ctgaaatgac cggcacaccg ctgtagtgtg aggcttcgaa
    12841 atccttatcc ccggtgacga agtattgggg agggggtgta gtggccatga gatcgaggaa
    12901 cggtcgatcc tgcgcatcac gtagcggagt gttggtggga tggggatcga cgaactgaac
    12961 ttcggcttgg atagtggcca gaaagctcgt gatattgacc ccggctcggg taaacgctcg
    13021 ttggagtttg gggcgccgca ggacggcttc cagttccgcg aaggtggcag ggctcatgat
    13081 agggatgagg cggcgctgta agacggcctg gatgatccgt gcaggaggcc ctgcggcgga
    13141 caacaggccg gacacgaaaa tattcgtatc aagaacgacg cgcacgctcg cgccgaaccg
    13201 ctgccacctc agcggcaatc tgttcaggag tgacgcccag ctcgcgggtg gcgcggcgca
    13261 gcttgctgac catacgggcc agcttcttac gacttgccgc tctggctgga gactccaaga
    13321 tcaggacttg ggagtcccga tgcgccacga cggctttccc ccatcccttc atccaggagg
    13381 agggaatcac cacgccttct ttggtgtact tcacggcttt catcagcagt gcatccagta
    13441 tatccgcagg cagtatacca tggcgctgcg gctatttcgt acatcagtcg gcgatcttgc
    13501 gagccgcccg cccgttgttc gtcgctgcat attcggacag ctcgattgcg accgtccctg
    13561 ccgagtcact tccttttaac ctcgcccgct gggtacgtcg ctcctttcgt ccgagaccat
    13621 agaatgcgtg tgctaaattt gtgctaaaaa ttttcaaaac cgctcatatt ggttcgaagt
    13681 agtagaatcc atggaacacc ggtaactgtt gaattccaaa tgaaaaacgg ggaaaccttc
    13741 gcaaaatcaa gccactgtga attctgctct gaatcgactc tcaaggctca gacacgggtt
    13801 caaatcccgt tgggaccacc aaccttttcc ctgatcctcc gcagggttag caactttcct
    13861 gagttccggc ctgacgaggg ctgacccctc ataggacaaa ataggcgtgg tgtacgtagt
    13921 ttctagctgc gtcacggcct cacggagctt cagattccac cccatctcgc agtgtgaata
    13981 ttgactcgtc atgaccgtct tggtgctgtg ccctaagagg gccgaacgta cccccagctc
    14041 accccgaggt tctgaagccg ggtcgtgagg gggtggcgga ggtcgttgaa atggaggtct
    14101 tggactttag ctgccttaca ggtccgatgc cacggatggg ttagcgctac agctggtggg
    14161 ccggcatgta ctattgaaaa gtgctccctg ttcgaggcgc gcaccttgtg ggacaaacgg
    14221 gcccgcgtga gtgcgtattc tttcacggca agggaagttt gacgctaatg gcgcctccca
    14281 gggtgccttc ctgtgcgcct tctcgcgcat atcccgtcac gtctcgctca cctttggggg
    14341 tgccatggca actcaggcag gccttgccat agaacagtgg aagaagcacc cgtacactct
    14401 tccctccgtc caccacttcg ctcacgattt gttctccatt gcgggggaac gacggatctt
    14461 ccatcttgtg cagcgcagca ctctcaaagg catccggctt gttcttggga ttacgaagga
    14521 ggccttcggg cgcggtttgc ttcagataaa tgccggacca tagttggaat cgagcccctg
    14581 tctccgtgcc gaacgtggct gggattaagc ctttgtactt gagccctggg acgttgatgg
    14641 cggtctgata cgtcgcgaca gttttcttgc tttcctccag gagccgttcg agaagtggct
    14701 ttgccagggc gggcacttga gccgtggaga gatttcgtaa gtcgtgtcca gtccgctctt
    14761 tgaacaaggc gatcatctgt tctgcaaaga cctcgggagt gaaacccttg tctcctctgg
    14821 cgggatcgtt gatcaggtcc tgattccgcc cgatcgccac acgaccggaa tccagcagta
    14881 ccgctatgag cctggctgac tcctgcacct gggcctcatc ctctgccgtg gccagtggaa
    14941 ccggatttcc caggaggaga gtagcgagcg ccataataag tccggttccc caacgacccg
    15001 caattgaaca cctcgatgat ttgcggtgat cgagtttcat cctgacaacc tcagctccgc
    15061 tcatattctt ccccctttgg tcgattggct cctatgacct caccatacct ctaaggtccc
    15121 agtagtatgg tgagtcattg ccgttgtcgt taaacctagg acagtgccta gtagcgcgca
    15181 ggtctcgagc cctagaccgt gcaatgtagc tatgaattcc atagggggtg cactacacgt
    15241 ctattaccga accgttcatc taaccaactt tgtcatctct ccaccagcgc ctgcactgtc
    15301 agcggggcct tcagtcgcag gtccttcgat ctgatggctc aagttcaatg ttttccatcg
    15361 aaggatggcg tgctcgagtg ccacgcctct tactacttgt gctgcggcac catgtgggga
    15421 gttcagtcac gagtttggac tcttgcgagg aaacgggcgc ttccattgac actataaaaa
    15481 acacggctaa ggtaagggtg gccgcacaga gatgtgggca cgatgtgcct tgcagtgtgc
    15541 ggtgacgcgc aagtacagaa aggagatggc atgaacaaag gattgttaat cgcgttgaca
    15601 ggggtgttga tgggtgttca ggccggcgtg gcccacgctg tcaatccgga caacggaccc
    15661 ggatgcggat tgggtaaatt ggcctgggcg gattacaaga cgccgaagaa cattgcccct
    15721 caagtcatga tggcgacaac gaacggtacg ttcgggagtc aaacgttcgg gatcagtttt
    15781 ggtacatccg gctgtaccaa tgacgggaag gtgatggccg ggcagcagac gaacgtgttt
    15841 gtcgccggcg cgttcgaatc cctctctgag gatatggcca gagggggcgg agaacatctg
    15901 gcgaccctgg cgacactcat gggcgtgccg tccgaacaac agtccacgtt ttttgcactg
    15961 gctcagcagc agtatggtgc gcttgttggg gccggtgaac tttcttcagt cgccgtcgtc
    16021 aaggcgctgc aagaggcgat gaaagaacag ccggtactcg cgcaggcatc aggcatccgc
    16081 tgagcgggtc tccctcttca gtttctcttg gagaaggtcg ccgggatgtg tgcgtctggc
    16141 ggtggatggg cgtgctctgc ctccgctgcc agccgcgccc cggcaagcgg ctcacgtctt
    16201 cgggaggcgg agtgcgcctt ggggctcctc cttcccacct ccggcgagac gcaccggctt
    16261 gaagcgctca gcctgacagg aggataattg tcctccgatg acccagccat cccacagact
    16321 gctgcttctg acgatcgtca tcctcggaat cggcctctac agccttgatc tctatcttcc
    16381 attaggcatc ggcaacgggg ttctgtacgg aggcctggtg gtgctgtcgc tggcatctcc
    16441 ggatcgcaag attcccctgc tcgtggccgg cgcctgctcc gtcctggcca tctcggatgt
    16501 ttttctcggg gtgaggttcc ccaatgtccc cctctggatg ggaatcagca accggctatt
    16561 tagtctggtg gcgatctggt ttcccgtcgt ctacgccttc cagcgcagaa aaattgaaga
    16621 agcgcttcgt gacgcgcatg gagcactgga ggttcgcgtg gaggaacgca cgcacgagtt
    16681 ggcgctggtg aatcaggcgc tgctcgggga gattgccgaa cgaatggaga cggagcggtc
    16741 actcagggag agcgagcgct cgctgaaggt gagtcagcag gagctgcagc agagtcgcga
    16801 agaactgagg gcgctggccg gtcgactgtt gacggctcaa gagggggaac gccgccggat
    16861 cgcgcgggat ctgcatgatg acgtgaacca gcgattggcg atgttggcga tggacctgcg
    16921 ccggattgaa aagggtgacc aaacagaccc tgcggccgtc gcgagcatgg tccgatcgat
    16981 caccggccgg ctgacggcgg tgtcggacga tgtccgccag atggcctacc gatttcatcc
    17041 ttcgatcctg gacgaccttg gactgacgaa agccgtgcgc cggttggtgg acgatttttc
    17101 cgccagcacc gggattcagg cggtgtatgt ccatcatgac cccgtgagcc ctgtccctac
    17161 cgacctggct acttgtgtgt accggatcgc acaggaaagc ctcaataacg tggcgcgtca
    17221 cgccagggcc tccgaggtcg aggtcgaatt gatttgcgat gaagggaggg ttactctttc
    17281 tattcgcgac aacggcgtcg ggtttgatcc gctgcaggtg tcacagatgc ggggtcgatt
    17341 gggtgtgctc agcatgaaag agcgggtgcg tttggtgcgg ggaactttgg atgtgacgac
    17401 ggcgcccggg cgcgggacgc acattgaggt ggatgtgccg ctggcaggga atgcgcatgt
    17461 ctaaaccgcg tgtgttgctg gcggacgatc attcgctggt gctcgaaggg tttcgccgca
    17521 ttctcgacga tcaatgcgac ctggtgggga tggtcgagga cggacgggcg cttcttgagg
    17581 cggcacaacg ggtccagccc gacattgtga ttctcgatgt gtcgatgccg ctcctgaacg
    17641 gcatcgatgc ggccacacag ctaaaaaaat cgcatccgtc catcaaggtg atctttgtca
    17701 ccatgcatgc cgatgcggac tatgtccggt cggcatttga ggccggggcc tcaggctatc
    17761 tcctgaaacg atcggcggtc gatgaactgg agcaggcgat tcgagcggtg tgggccgggc
    17821 atacctacat caccccgttg atcgcgaagg ggctgctcga cgtgctgatg acgatcggcc
    17881 cggggcagtc acagcagaaa aaaacactga cgttccgtca gcgtgaagta ttgcagctgc
    17941 tggctgaggg ccgaacggta aaggatattg cgattcgcct caagatttcg acccgaaccg
    18001 ttgagtttca caaggcgcag atcatggaac aactcaacct ccgtaccacg gcggagttga
    18061 ttaagtatgc gctcacgcat gggattgtag ccccttccta acgctccctt cggtatccac
    18121 gagagatcca cccgagggtt cctgcatcag gacctcgtaa tcttccgctt aagactgtca
    18181 ggccttacga gttccacatt cttcggggct gttctataag gaatggcttg atgtgccgat
    18241 ccaagcagga gggtgtatgc gcaccggaga ccgagaggag gtaggcgaag gatcgccaca
    18301 attcttcggt cgcccggtgt tattccgctg tcgctcctgt gagcgggtcc ggcttgccgg
    18361 actgctggag atgtccctgg ctacctggca ggatgaatgc gcgatgctgc ggggccagga
    18421 gttcagtcgg gaagatgtct ggtggttcca tatctgctgc gaacgttgtc agcctaccga
    18481 gcacacctcc gactccgtct agccccgcgc cgatcatcct cacaacgatc tttcttcccc
    18541 gccgccggtt ctggtagaag tctgacatgg tgaatgtgat tggactgggc gcgatcgccg
    18601 gtctcattcc ggtctatctc ggcatccttg cggccctgtt tttaggcaag gtgttgccgc
    18661 ggacctggga gggtgggctg atcggcgtgg ccaacggtgt cctggtatac cttttcttcg
    18721 atctcatgca cgaggcgacc gagcagaccg gcgcacgtga tgtgacgtcg tgggcggtgt
    18781 ttctcgggag tctgggggtg agtttcgtcg gattggtggt gttggaatcg agccaggtct
    18841 tcggcgggcg ctcaggaaat cgcatgctgt ctctgcccta catgattgcg gtggggatgg
    18901 gcttacataa tctcggcgag gggctggcca tcggcgccag ctacgccagc ggtgaatata
    18961 cgctgagcgc gttattggtg gcgggtttcg gtctgcacaa cggcaccgag ggtttcggta
    19021 tcgtgggcgc ggccggcaaa acgccgatgt cctggcgcga tgtcttcttg ttggggttaa
    19081 tcgccgggct cccgacctgt gtcgggacat ttctcagcgg acaaggggta tcttcctatt
    19141 tctccatctg cttctacgcg ctggcagccg gttcgttgct gtatgtggtg ctctcgctga
    19201 cggtcatgtc ctatacggcg acgcgtcggg tgcaggttgc agcgggcatt tttgcaggta
    19261 tcagtctcat gtatgtcacg gcgatggtgt tagcattggt cagcggcgtt cgcagctgac
    19321 ggcccgcgcc atacacaacg tctttcccct cccgttttat tcgcgcagcc ccagcgtaat
    19381 gttcgtgttg ttcacggtgc atctccgcgg tcagacagct gggcaaccgc ggacgccccg
    19441 ccggtgcttc aattgacccc gcgacgctcg gttgtgtata aaatgacctg ctcgatcgga
    19501 agactgattt tttcgggagg ctctcatcat ggcgggacgg gaatttcacg acggtgccac
    19561 ggatggcttg gaggcgctcg agcctctcga ccccgagaag atccattctt tttcggaact
    19621 gctggaggcg atgcggaaga cggccttcgg tggccggcgt ctgggcgaag catacgaaac
    19681 actcgccgca atgatcgagg atgccgattg caaggtggtg ttgacgcttt cgggcgccat
    19741 gaccattgcg aagatgggca agatcatcag caccatgatc gaccgcggca tggtgcatgc
    19801 cgtggtctcg accggcgcgc tcgttgcaca tggcctgagc gaatcggtgg gcaagcttca
    19861 ctaccgtcac gacgcgtctc atagcgacga ggagttattc cagaagggat acaaccgcgt
    19921 ctatgatacc ctcgaaatgg agtcgaacct caattacgtc gagcatgtcg tttcattgac
    19981 cctgaaacgt atcaacaccg aacaacccct ctcttcacac ctcctcaccc gggaactcgg
    20041 caagacgctg gccgaagaat ttgagggccc cggcatcctc aagagtgcct atttgaaaaa
    20101 tgtgccggtc tatattcctg cctttaccga ttcagaaatg gggctggatg tcggtacctg
    20161 ggcgatgggc aagcggatgg atcaggctcg cagccaagtg aaggacggtg gagacacggc
    20221 ggtcttgcgc gcgctgcacc agacctgtcc ggacttcaac ccctacctcg atctcaatca
    20281 ttacgcggag gaagtcctgg ggtcgaagcg cctcggcatt ttcaccatcg gcggcggggt
    20341 gccgcgcaat tgggcgcaac aggtggcgcc gtatatcgag atcagcaaca ctcgtttggg
    20401 actcaacgtg caaccgccac gctttcatta tggcgtgaga atctgtcccg agccggatta
    20461 ttggggtggc ttgagcgggt gcacctatca agaaggcatc tcctggggca agttcgtgcc
    20521 gccggctgaa gggggacgtt tcgccgaagt gctcagcgat gccaccgtgg tctggccgct
    20581 gctcatggtc gggctactgg agcgaatccg cgccaagagt gtcacggcat gacccccgtg
    20641 acggcgtggc tgcttcgggt cggggcgtcg tgtttcctgt tcttgcttgt cgcgggagcg
    20701 gctctggctg cgagtgcgcc ggcggtcgat catcgtatgc ggggcaaggc ggatgcacca
    20761 gtgacgctga tcgagtattc agacttcacg tgcgggtact gcctcaagtt tttcaaagaa
    20821 acctggccca agattcaggc gcggtatgtc gacaccggca aggtgcggtt tctctacaag
    20881 gattatcccc gcgcagatca ggggccaggg gtcaccgctg cgctcgccgc tcgttgcgcc
    20941 ggcgatcagg gaacctattg gccgatgcat gaccgattgt tcgcggcaga cgggcggttg
    21001 gatgtggaca gttactccca acatgccaag gcgatcggac tcgaccagtc gcaattccgg
    21061 caatgtttgc gcgatgctcc gcatatgcag gcgatctttc acgaccggga tgaagcgaat
    21121 tcctggggat ttcacggcac acccggcttc gtcctgatgc gcacggccca gcagccgacg
    21181 accaagaatc cggctattgc gattcccggc gcgttcccct ttgaagcctt cgaggaagaa
    21241 attgagaagc tgctcgcgcc ggcgggggcg aaacagaaat aatgtttctc atgcggaaga
    21301 aagccaggac tgtgacgaga ctcatcgttt agccaggaaa gaggaaactg cactatggcg
    21361 tcgaaacaag ccttttggtc tgtgcctgcg aaggatggag agcccgattt atgggtgtgc
    21421 atgtcctgtt tgagcgaggc gttttgccgg aaggtgccca tgccggcctg tccgacgtgc
    21481 catggtgtgt cgacctatga agcgtttacc ttggcggcgg ttcaggattg gggaaccgag
    21541 gaattaatcg ccaaggcggt ggcggctcag cgcgatgcgg aggcgatcag cgccgcagcc
    21601 cccgcaacac caccactgga atccgtgcag tagcgattct ccctctcctg gagtaagtcc
    21661 gttggaagat ggacgtccat ttctgatcgg tggtcggtgg tctcacaccg ctacgactgc
    21721 tcccgtacgc aatccctaca caggcgagac gattgctcat gtctgccagg ccggcccggc
    21781 cgaagcggaa gcggctgtgc agtcgtcggt cgagggcgcg gtcgcgatgc gccggttgtc
    21841 cggctatgcg cgctcgacgc tgcttcagaa cgccgctcac gcccttcagt ccaggcagga
    21901 agagtttgcg cgcaccatga tggccgaggc cggcaagcct ctgaccgatg cgcgtcgcga
    21961 ggtcggccgt gccattcaga cgttctcgat cgctggggaa gaagccaagc gcatcggggg
    22021 tgaagtggtc ccgctcgatt ggtctcccgg gatggagtcg tactgggggg tgacgcggcg
    22081 atttcccatc ggacccattc tcgggattac cccgttcaat ttcccgctga atcttgtcgt
    22141 ccataaggtc gctccggcct tggcggctgg taactcgatt ctcatcaaac cggctccgca
    22201 gacccccctg acatcgctcc tcctggcgga gctgcttctc gaagcgggcg ttccaccggg
    22261 tggtctcaac gtgttgccct gcgataaccg ggtggcggag caattagtcg tcgatccccg
    22321 gttcaagttg ctgagcttta ccggaagcgc gccggtgggc tggatgttga aggccaagtg
    22381 cggcaagaaa aaggtggtgc tcgaattggg cgggaatgct gccgtgatcg tcgagccgga
    22441 cgcggatctg gattatgtgg cgcaacgttg tgtgaccggg ggattcagct atgcggggca
    22501 gacgtgcatt tcggtccaac ggatttttgt gcacgagtcg atcgttgcag cctttaccga
    22561 gaacctcgtg gcccgtgtgg agcggttgga aaccggagat cccggcaatg aacgcacggc
    22621 cgtcggcccg ctcattgatc cgggggccgc gcagcgcatc gaaggctgga tcggggacgc
    22681 cgtggcccaa ggtgcccggg tgttgtcggg cggaacgcgg gtggggtcgg tcgtgcggcc
    22741 gacggtcttg tcccaggtga cgcccgccat gaaggtgtcc tgtcaggaag tgttcggtcc
    22801 gttggtgacg attacgccct atcgcgagtt cgatgacgta ttgaaggctg tcaatgagtc
    22861 ggagtacggc ttgcaggcgg gcgtgttcac cagcaacatc gggcgaattt ttcaagcatt
    22921 tgagcagctg gaggtcggcg ccgtcctggc gaacgaaatt ccgacgttcc gcgcggacca
    22981 tatgccctac ggcggggtga aagattccgg gatcggacgt gaagggcttc ggtatgcgat
    23041 cgaagacatg accgagccca agttgctggt catgaacctc cgcagaccct gaaaggccct
    23101 ggggcgaggc aaaaaaaccc tgccgccgta ttgcggatgc cccacaaact tgatacaact
    23161 ttcgcgcgtg tcgccgcatg atcaattgcg tggcacaagg cttgtgacaa ttcttcgaac
    23221 actcagggtg gagtgatttc gacgagggat gaagagggag acctacatgg taccaacaca
    23281 catgtttttg acgaggggcg tcggggtgca caaggaaaag ctggctgcgt tcgagcaggc
    23341 cttgcgtagc gccggcgtgg cctattgcaa cctcgtcagc gtctcgtcga ttctcccgcc
    23401 caattgcaaa attctccctc gcaagcgtgg cgagaaattg ttgaatcccg gtgaaatcac
    23461 gttttgcgtc atggccagat cggagaccaa cgagcgcaac cggctgattt ccgcgtcgat
    23521 cggtttggcg attccgacgg atcgggatac gtacggctat ctctctgagc accatgcgta
    23581 cggtgaaacg gacgaagagt ccggagaata tacggaagac ttggcggcac agatgcttgc
    23641 gaccacgcag ggcatcgagt tcgatcccga tgtggcctgg aaagagcgtg agcaggtgtt
    23701 caagatgggt ggaaaaattg tccggaccct gaatatcacg caatcggcgg tcggtcgtcc
    23761 gaatcgatgg acgtgcgtca tcgccttggc ggtgtttatc cccacggaaa acatccccaa
    23821 gagtcttcga gacaaggcct agcggcgggc ggcatggcgc tcccgtccgg atggcttggg
    23881 caagccgaca actttctggg gatcgacgaa ccctggtgtc atcccgatca ggccggagtg
    23941 tacgtactcc cggctcccta cgaacatacc tccagttata tcctcgggtc cgatcggggc
    24001 ccctccgcga tcatcgaagc ctcccaacag gtcgagttgt acgatgagac gctacgctac
    24061 gagccctacc gcgaatgggg cggcgtggcg acggcagcca cattgaacct cgatgggcgg
    24121 gtcgatggtc cggccgtcca ggctattcag gattttgtgc agccgcatgt cggtcggggg
    24181 aagtttttgg tcaccctgac cggtgaacat accggcgcct taggggcgat tcgggcgcat
    24241 gcccggaatt atccgggtat gtgtgtggtg cagatcgacg cccatggcga tctccgccag
    24301 gcctaccagg acaatccata cagccatgcg agtgtgatgg cccgtgtggt ggaggatggg
    24361 ctgcccctgg tacaaatcgg tgtccgatcg atcagtccgg aggaaatcga tctcattcag
    24421 aagaccccac ggatccatac attttttgcc gcctcgattc tcgatccatc cggtccctat
    24481 gaggggcggg ccgctcgttg gattcccgag gttctggccg cctgcaccgg gccggtgtac
    24541 ctcaccttcg attgcgacgg gctcgatgcg tcgctcgtgc cggcattggg aacgcccgag
    24601 ccgggcgggt tgggttggta cgacaccctc gcgctggtga ctgcgttggc aaacggtccc
    24661 ggcatcgtcg gcatggacat cagcgagatc gcgcccatcg aagggtttgt cgcgccccag
    24721 ttcagcatcg cccgcttgat ctatcggatg ctgggtcgga ttcgcgccgg ccgccgcgcg
    24781 cattgactct cacattgtga aacgtcgctc ctgaagcgtc tctcgttgga gagcggaatg
    24841 gatcgctcat gacttagaac acaggcaatg agagagctct gcctgtgttt ttcggtcgag
    24901 gcttttgttc atacgctctt tgcgagatac gacagacgct tcaggaggga cggctcgcgc
    24961 gagttcattc cccgccatgc gcatcaataa atttttcacc gagcagggcc tgtgttcacg
    25021 gcgggaggcc gatcgcctca ttgccgaagg gcgggtgacc atcaatggcc aggtggccaa
    25081 gctcggcgac caggtctcgc cacaggattt ggtcgcgcgc gacggcgttg tgctgcagcg
    25141 cggcaatcgc tcggtgtatc tcaagtatca caaaccggtc ggggtgacga cgaccaccga
    25201 accgcatgtg cggcggaaca tcatcgccga aatcggccat acggccagaa ttttcccgat
    25261 cggacggctc gacaaagatt cttccggact cattctgctc acgaacgacg gcgacatcgt
    25321 caatgagatc ttgcgggtcg agcacggaca tgagcgggaa tatcgcgtgg aagtggatcg
    25381 ggaattcgac gaaacgtttc tcgtgcggat ggcccagggc gtgaccattc tgggggtgcc
    25441 caccaggcct tgcatcgtgg cgcggcttgg cccccgtcgc ttccgcatca tcctgacgga
    25501 agggcgcaat cggcagatcc ggcgcatgtg tcaggcgctc ggttatcgcg tcgtgtcatt
    25561 acatcgcgtg cggatcatgc acattaccat tgagggtttg catgccgggc agtggatgga
    25621 tttgacccac gatgagcgta gccgactttt tcaagctctc ggccggtcgg ctgattcgtc
    25681 gatgtaggcg ggggcgggag tggtggtagc tcgctctgaa gcgccgggtg gttatccctc
    25741 gctcggcagg ctccgtggtg atcccggatg gggcgtagct tccggccttg aagagggcag
    25801 ttcctgtatg cgcaccggac gggtgagaaa caggttcaac agcgcgatga gaaaactccc
    25861 gcccacgatc cagtagccgg tcgtcacaat cgttttggtt ccttcgtcgc gcatgtcctt
    25921 ggcgacctct gccaccgtct ccagcaggcg gtccagcgcg agactcacct gcatgacttt
    25981 cggcccgcca ttgtcggccg catgaatttc agctttgcga cggagttccg ctgcttcggt
    26041 cgcggagccg gcactccact cctgggacag catatcaatg gtcttgctgg cgacatggaa
    26101 gtactgatcg aggctttcgc gaaccgcttg aatgtctttt tcttcgctcc gtccgctccg
    26161 tgacacacgt aggccggctg cggcgtatcg atccacggca tgttgaatgc gtgcgcgttg
    26221 cgtcggcaac gattcggtaa ttcgttcgaa ggtggtctgg ttctcggcct ccaacgaccg
    26281 gatgatcgta tttcgatagc ggatgacgtc cgctgaaata tgggcgaggt ccgctgcgcc
    26341 cagggtgtat tcggtgtaca tgatgcgcag gtcctgatcg atacggctga gagcctggcc
    26401 gctgacccat cccaatcccg cgatcagaag gctgatgatc agttggttcg tagtgggaaa
    26461 ggacaatttc aggcgtgatg ccaatgacac aggcgttccc ttcgtcaggc tgcagtcgaa
    26521 aactggcgtt cagtctagca gatcgctcgc gaagaaagaa gtcgagccgg ttttgatgca
    26581 gaacgggccg tcagtccagc accacccgtc gctcgaccag ctggctgacc tccggatcgt
    26641 tggagacgaa caccaccgac cagggttctt ctttcgaaca gagtcggcgt agcagggtga
    26701 gtcgcagcgc cggctcgatg ttgtggaggc tgccgttgaa gatcaacagt tccggacggg
    26761 tgacgatcat gcgggccagg agcacgcgga gcacctggct acgagtcaag ttggcgccat
    26821 ttccatgcac cagagtttcc agcccctgcg gcatggcgtc gatttcctct tcaagttcca
    26881 cgaaccggag ggcccagctc agatcttcaa actgaatcga agggcgctgc agagtgatat
    26941 tttcctccag ggatccgtcg aacagggtgg gatgggagtc cagcatcagc ccccggcagc
    27001 ggttcacgta attcagcgac acatcgcgca ggtcaatgtc gttgtagcgg atcaccccgc
    27061 cggttggatg gtacagtccg gctaacacaa gcgcgagcga ggttttgctt ctgctggttc
    27121 cggagaggac gctgatcttt tcccccggca tgacttccag gttgaatcgc tcgaagagca
    27181 gcggtccgtc cggcgcggcg aaggacacgt ctttgcacgt caggcgcacc ccctgcagcg
    27241 agggatcggg gagccaggcg gccacggggc cggagacatc ctccttcggc atatcaaaca
    27301 gagtggagag ctcatggagc gaggtggcga catagatggc ggcatacatg cgccgggcga
    27361 cggtgtcgag attgagcaac agggttccga cgatcacctc cgccgcgacg aactgtccga
    27421 gggtgatgtc gccgaccgac agcaaccagc cgccgaggcc gatcatgccg ctatgcgcca
    27481 cggcctgaaa aatgaccgta ctcttatatt gcgcaccgct cagaatatcg gaacgcgtct
    27541 tccgcgccat cacataagcc ttgaccagcg catcggtttt cttgaggagc aacggcaggc
    27601 tgtccgtgga cttgaaatgg agccgattga tgccgatgtc ctggagccag tgaaaggtct
    27661 ggtagtgcag ccgggacacc cgttgtgtga tgcggagccc gcctcgaccg aaaaacgtca
    27721 gcagaaaggc aaaccctgtg atcaggaggg tgttatatcc caggaagtag ggatggtaca
    27781 tgatcaggat cgccatgccg atggcgcccg acaccgacac attgatcaca tcgaccagca
    27841 tggcgaccag ggcccggggc aacagttccg cctcgataaa cgtgttggta tgttgaggaa
    27901 gaaagacatt ctcgcggaag cggggcaggg cttcggtaaa ggcgacggcg agccgcgtgt
    27961 agaggcgttg gaccaggatt tccaccgccc gcgcttgcag gacccggaat gcgcccatag
    28021 cgagcagcgc gccgaacatc gtggccacca gcgtcacgat catgatcggt tgaatcgcga
    28081 aggcgaaggt gttgaccagt tcttgcactg tgagcgggac gatcaatgca aacaggccga
    28141 tcacgactgc ataggacacg acgatggtga ggatgcggcg ttcaagcccg atcaggagat
    28201 tcaggcgggc gaggatggtc caggcggagg gcgtcgaaat gggcgtttca gtggcggtgt
    28261 agttcacaca agggctcgta tcggtcggct gtgggccgca gtgtgggcga ttgcctgatt
    28321 cctgtcaagg ttgaccaggc tctccggtcc ggcggtgcga agggctggga cttggagcag
    28381 ggcgggcgca ggcagatgaa tggaggcgtc atccggctag ttcaacatga tacggcgatc
    28441 gacgtgcggg gtcaggttgg gatcgttcga gatgaaaatg accgaccacg gctcttcctt
    28501 gctgcagagg cgccggagaa tcgtctcgcg catcgcggga tgcatgttgt ggaggatccc
    28561 ctcgaaaatg agcagttgcg gccgccccag gatcgcccga gcgaccagga tgcgcacaat
    28621 gtgcgtcggc gcgaaaattt tcccggcagc ccggacgtgg gttttgaggc cgtgcggcag
    28681 agcatcgact tcctcttcca gctcggtgaa gcgtaacgcc catcgcacat cactgtaggg
    28741 gatatactcc cgcccgagca cgatgttctc ttcgagcgtg ccttcgaaca acgacaactg
    28801 cgaatccagg atgaatcctc tgaaccggtt gacggagtcc aggttgaggt gccggaggtc
    28861 cacgccgtta tagcggatca caccggaggt gggggattcc aggccgccca gtacccgggc
    28921 cagcgcagtt ttggccatcg tgctgtccgc ataaatgccg attttttcgc cgggcgtgac
    28981 ttccaggttg aagcgctcga agacggctgg gccgccggcg ggggcaaacg tcagatcttt
    29041 gcaggtgacg cgaatgccgt gcacggtcgg gtccgggagc gggatggaga gggtcgccgt
    29101 atcctggtct ttcgggagcg agaataagaa gctcagctcc gtcaacgccg tcaggaagta
    29161 atagatgtgt cccatccgtt tcacgacccc gtcgaagctc gacaggatgc cgcttacgac
    29221 gacttcggcg gcgacaaatt gtccgagcgt caattgcccg atgcccagga gccagccggc
    29281 cgtcgcaatg agaccgctat gcgcaatggc ctgccatccc agtgagccca gatactgccg
    29341 gatcaaaatt ccgaatctgg tccgacggat gccgatgtag gtatccagga gctggtccgt
    29401 cttcttgacc agcagggcct ggctgtcggt cgacttgaaa tggagcaggt tgtaggcaat
    29461 ttcctgaaac cagtgcaggg tgtcgtactt ggcatgagag aggttcatgt cggccttgag
    29521 gccgccatgg ctcatgagga agaagaccac gttgaatccg accagcagga tcgcgttata
    29581 cagcaggaaa tacgggtgat aaaacaccag gatggtcatg ccaactgcgc cgccgacgat
    29641 gacgttgatc agatccacca gcaggaccga gaccgcccgc tgcatgagaa tggtttcgac
    29701 gaagaaattt gcaaagcgcg gtttgaagcc ctggtagcgc aggtgcggga gttgctgagc
    29761 catggcaatg gcgacccggg cgaaaatgcg acgctccaac acttcgaccg cgtaatactg
    29821 cagggcccgg aaggcgccga cgaagagcag ggcagccacc atgacggcgg ccagggtgac
    29881 gatggtaatg ggttgaagcg cgaacgcgaa ggtattgacg agttcctgaa ccgtgagggg
    29941 cacgatcagg gagaagagtc cgatggcgat cgaataggaa acgatgagtc cgaggacgcg
    30001 ccgttccagg cggaagagga ggccgagatg gccgatgacg gcctggaaca gattgctctg
    30061 attatcggaa tggtcctggg ccatcgtgtt actccagcaa aacctgaccg ggccccttgc
    30121 cgtgtctctt cgcgagcagt ctgaagccgt ggaggtgaca cgcctgtgcc ggaccgcctc
    30181 gtcggctaaa acgtatcaga gcagaccgta aaataccact atttggagaa gctcgccccg
    30241 acgcggtagt tcaccggtgt cgcggatggc tgactccgtg cccagatgcc ggtagcccat
    30301 tgatacaggg ccagcgcctt ctgatagtct gccttcgctc gcaccagctg catttccgaa
    30361 tcgacggagt ttcgttcgcg caggttcaca aacaggacgc tggtggcgcc caggctgaac
    30421 cggaaccgct cgccttcctc aagcgtcttg gccatgcgga gcgattcggc cgctgcggta
    30481 acccgctcct tcgcgcgttc gatggccgag agcgcattgt cgacatcgac gaccacttgc
    30541 tgttcccggt atttctgcac catcaccagg cgatcggcct gtgcctgcgc ttccatcact
    30601 tcaccgcggc ttctccgctg caggatcggc atgcgcaact ccacaccaaa ccggtagccg
    30661 aggccgagga cgaatttctc cggcgctcgc gccggcgccg cttcaagatc gaggctgggc
    30721 agcaggttgt ttttggccag ttccaggtcg atattgttga ccttggcttc gatatcgact
    30781 tcgcgaattt ccggccgatc ggtttttgcc tgcagtttgt cggctcgaac gatttcaggg
    30841 gtcggcacgg cggtcggggg aggaaaatcc ggcgctcgct cgacttgcgg cgccgttggc
    30901 gtctggtttt cccagaggaa catcgacatc ttatattgct cctgctctac ggctcgttgt
    30961 gcgccgatgg cgatctcgcg ccggcgctgg acttcctgcc ctgcttcaac gacgtcgagc
    31021 ggggcgacgg cgccggcttt ggctctgcct tcaacctgcc gcagccgttc ttccgcgact
    31081 cccagggccc ggcgctgcac gtccacgtac tttgccgcag agacccaatc ccagaactgg
    31141 gtggcggctg cgaggaacag gtcctgcctg gtttgggaga ttttgacatc ggcgcgtgga
    31201 tcagccaatt ccgaccgctg gagttcggca ttctccgggt tgatcatgag tcctttgagg
    31261 aggggcagga agccaccgag aatgacctgt tggccgttgc cgaaaccgag gtcgggaatc
    31321 ttggcgtcac caatggactg ccgaacgccc gcgctgccac ggaaccccca gggcgtgcgc
    31381 gcttcgacca gggtatcgtt gaagccgacg ctttgcgttt tagtggtccc tttatcgata
    31441 aatcgttcga tttctgtatc gttcacgagg acgggctcga aggcgcccag ggctttgagc
    31501 attttccccc gcgccgaagt tttttcggtg ccggctccct tcaggagcgg atgggaccga
    31561 tcgatccagg cgtggacttc gttcaacgag agcggaatgg gcgggagcgt gcgtggtttc
    31621 gccgtctcgt ccgccgacag accggtgtca ggcagcgagc agagtccaca ggcggcgagg
    31681 gcaagtaccc aggcatggat cttcatgaaa cgcactcctt tctgagtata aacaagagag
    31741 aacaaggtcg aaggaaatga gcgcttcggc ctggcggccg gtctgaaggg gcctattact
    31801 tggccccgcg accggctttc ggcaagagcg tatcgatcaa gctcgggggc cgttcctggt
    31861 agtcgggcgg aaacagattg aagcggcgcc acagctcgta ccagagcggg acacgattga
    31921 gaatgaccca ccccatggct ttggtgccct ggcggacatg ttcttgcgga ggccagggac
    31981 gttcctccag gtcagggacg acccagaagc gaaagttgcc ttttccgtcg tccacctggt
    32041 cgatgacctt gactacgccg ttataggtac cggccatcag ttccggccaa gccggcaatg
    32101 ggatggcggg aataccgtag aagagaatct tgaccttccg gccgacattc aacagcggcg
    32161 cgtcgatgcc gtcggcgacc atttcgatgg ccttgtccgt gctgcttggt gaaattctga
    32221 cgagtttgtc cccgggacgc acggtttcgc ccgcgccggc ctgcgccatt ttcaccacgg
    32281 taccgtcgat gggagaaaga atgcggccgg ccacgcgccg ctggttcgcg ttggagaggc
    32341 gtaacgatac gtccgcgact tgatcggcgg ctcgcgctgc ttctccgatc gacgcatcgc
    32401 gggctgcttc ggcgtcgagg agacgttgga ggacttctgc gcttacttgt tcgcgtccga
    32461 aactcaaggc tttcatgctc tgttcggccg cactcaaatt ggcctgtgcg ccttgcaagt
    32521 cggctttact ggccagggca gattgaatag tgagttccag ttcgcgttgg gagaccaggc
    32581 cttgttgcgc caattgctgg tgccgttcga cgttgagttc ggcagtggcg acggcaatct
    32641 tggaggcttc gattttctgt ctcgcttcgc gcaccttgct ttccgcctcg aggacgcggg
    32701 cacctgccga agggacggct gcctttacaa ggttttgcat ttctttgatc cgcttatcga
    32761 gctgatcggc tcggctcaac gccgcctttc gggtgtcctc cagtgctttc ttgcgttggt
    32821 cgagcaggct caagagatcc ggggccatga agttcggatc gtagtcatcg agttcgagaa
    32881 tcacatcgcc ggccttgact cgcacgcctt cgaagacgtg ccattttctg atgcggccgg
    32941 tgatctgtgc ttcgacgtcc tgcggccgtt cgaatggtgt gtaggccgaa agctgtccgg
    33001 tcacggtgat ggtttgcgtc cagggtacga atgcgacgat gagaatgaag aagccgacca
    33061 ggagaatggc cagccgggag gagaagcgaa gccgctcggg aatctggacc gccacccacg
    33121 actggagctg ggtcgatatc gcaagatcat cgaccgccat ttcatccagg cccgtgcgct
    33181 gggtcaccag ggagcgaatt ctgcttttga gcccttcacg gatccgctgc acgtgtcccc
    33241 tccagggcac aatcagtgac acaaaaattc acaacctatc atagtggatt gcggaggact
    33301 tggtcaattc aatgaggggt tccgccgggg tgcggcttga cccttcgacc ggagaacggc
    33361 taaggttcgc cgcatcgttc tttccttcag ccaaaggtgg cctctcacat gtcaaagaac
    33421 aatacagcgg tccgggcttc tgtcggggtg ctgggtggaa gcaagtcgga tttcccgatc
    33481 ctggaaaagg ccgtagccat gctgaacgaa ctcgggattc ccaacgagct cttggtggtc
    33541 tccgcgcacc gcactcccga tcgtctcttc gcctacgcgg aacaggcgat cgaccggggc
    33601 atccaggtca ttattgcggg cgccggcggt gccgcccatc ttcccggaat gttggcggcg
    33661 aagacttcct tgccggtgat cggggttccc atccccacag agaatctcag aggattggac
    33721 tcgctgttgt ccatcgtgca gatgcccaga ggcattccgg tggcgacggt cgccatcggg
    33781 ggcgcggaga atgccgccat cctggccgcg caaatactcg gactccggtc ggccgccatc
    33841 agacaacggg tcgaacagtt tcgcgccaat caaacacagt cggtgctcga ctcacaggac
    33901 gacgcccgat tgtctcctcc cttgcggatg ggacgaagct cccgggcgag ccgccggtcg
    33961 tgaacgtgac ggtcatcgat cccggcgcga cgctgggtgt gctgggaggc ggacaactcg
    34021 gcgccatgtt cgcaaccgcg gcccggcgtc tcgggtatgc ggtggtcgtg tgggatcccg
    34081 atccagaagc gccggcccac cggttggccg actcctccct tatccgatcg tttacagact
    34141 ctgccgcgcg tcaggacttt atccgacgcg tgtgtgctgt cacgtatgaa tgggaaaatg
    34201 tgcccgccga cctctgcgag cagttggaac gcgacgtgcc tgtccggccc tcgagccgag
    34261 tgcttcgtgt gattcaggat cgaatcgagc agaaaacctt tcttcggact catggattgc
    34321 ctgttccggc gtttcacgcg atctcgtcgc cggaggaact cagtcggcag agagcgctgg
    34381 aatatcctct tgtctgtaag actgcgacgg ccgggtacga tggcaaaggc caatggaaaa
    34441 ttgcgcgcgc agaggattgc gccgccgtag aggaggatct ggctcgaagc ctgcgccccg
    34501 gttcacgctg gattctggaa gcctggttgc ccttcgaacg tgagctttcg attctggtgg
    34561 tgcgaggcat ggatggggcg gtgcgagcct acccgctcgt tgaaaatgtg catgaagaag
    34621 ggatccttcg tcagtccact gttccggccg atgtgccggc cacggtggcc gaccaggcta
    34681 cggtgatggc acggagcgca gtggaggctt tggacggcgt gggggtgttc tgcgtggagt
    34741 tatttctcat gcccggtggc cggttactca tcaacgaggt cgcgcccagg ccgcataact
    34801 ccgggcacta ttcgttggac gcctgtacgg tgtcgcaatt cgaacaacag gtccgcgccc
    34861 tctgtgggct cccgcttggc gaagtgcgcc tgctgagccc gaccgttatg ctcaatctca
    34921 tcggtgacga ggttctgctc gcaaccgtgt cgcctgctgc gcaagatcta cttcgtgagc
    34981 cgggcgcggt ggttcatctc tatgggaagc gtgtggtgcg cccgaaacgg aaaatgggcc
    35041 acatttcgtt cctggccgcg acccgtgaag cagcactttc aaatgcatcg gtcttccgat
    35101 cccgcctcgc ccggtatcac tcttaagcac cctgcgccac gcgaggcgca tcggtcgcag
    35161 ccgacgcgct gctcattcgc atccgttagc gtagcccgtc taacccgggt ccgttcgttt
    35221 ttgtgatcag gtgagaacga tccggccctt cctgagcacc tgtccgcttg cgcctcctga
    35281 taatgcgacg acttgccgtt cggtgatgca gggtacagga cttgctgatc ttccggcagg
    35341 cttcacactc ttccaccaaa ggcaggcgcg gtcgtgagcg ttccactttc ctcttccgat
    35401 ctcgatcaaa ccggacgcca tcccaacata cgagggttgg ggcggcttct cgatcattcc
    35461 cgaacccgca cgatttttct gcaaagtctg gtggccggta ttctctcgta cgaattgctg
    35521 gtcagcaatg agacgatctt cggacatcgg gtgagcctca tggtggccgc cggcctgatc
    35581 ttggtcagct gcggcatcat gctgttgccg cggaacctcc ttgagagcgc ctggtttcca
    35641 ggcgcgctga tcagcatcaa tacgctgttc gtgacaggga cgatttatct ctccgggacc
    35701 gccagttcgg agctgtatct cacgtatttt ctcctcctct tgatcgcctc gtcggccccg
    35761 tcgctcaggc aactactcgg tctttcgacg gtgatttgcg ccgggtacgg cgtgttggtc
    35821 tacgaacatg cgatgcagtc gggtgggctc gaggtggggc atctcttagg cattcccgtg
    35881 ttgctgatca tgtcggtctt ttatggattg acgcttgaaa cggtcgccac cgaacgccgg
    35941 cgcaatcgcc tgttgcggga aaacgtacag ggattaaagc agtcggagca ggtgttggag
    36001 gagcgacgga cgcaactcga aacccgcgtg cagggattga agcaggggtt gtcacgcgcg
    36061 aatcaggaaa ttcgtcaggg catggtggag cggaccggct tggagcgaca gcttcgcgaa
    36121 gcccaaaagt tcgaggcggt gggccgcctg gcctccaaat tggccaatga gttcaatcag
    36181 gttctggcgg tgatcgggag tcaaaccgga gtcattgtct caaagttgaa accggatgac
    36241 ccgctgcagc ggccggtgga agagattttc cgaagcgggg aacgggctgc cactctgacg
    36301 gcgcaattgt tggcgctacg cgttcatgag acggcgatcc gggataccct gtcgctgagt
    36361 ccggcaatcg agtcgatgcg ggacacgttt gaggcattgc tgccgggtcg catcgatttg
    36421 cggctggcga acgagtcgat tccggtcctg gtggagatcg ggcatgaccg cttggagcag
    36481 ctgttattgc acctggtcgc caatgcgcgc gacgcgatgc cgaagacggg acgggtggag
    36541 atcgcgctgg aaatcgtcac cggcgaactc cttcccgccg agcaactgga aaagaatccc
    36601 cggaagcgca tggcccggat tgctgtgagc gacaacggcg gcggaatgaa cctggatgtg
    36661 caatcacaaa tgttcgagcc gtttttctcc acgaaggaca cccacgccgg cctgggactt
    36721 acattggtat acgggatcgt ccggcaatac ggggggacgg tcgaggtgca gagtcagccc
    36781 gggcaaggga cgacggtacg ggtctacttt cccctggtcg aacggaacgg ggtggtgcgg
    36841 gatacgaagg tcctcagcgg ggcgctctca aaaggggccg agaccgtgtt gctggtggag
    36901 gaagacgaaa ttacccgaaa actggcctcg tcgacgctcc agaaccatcg gtaccaggtg
    36961 ttagaggcgg ggtctgctgt cgaggcgttg ttggtggctc agcagcatgc tgggccgatc
    37021 cacctcacgg tcagccatct ctcgatgccg gagatcagtg gccgggaatt ggccaagcgt
    37081 ctggtccttc aacatcccaa gatgaaggcg ttgttcgtgt cggggttctc cgacgacacg
    37141 atcgcaagcc atcgggtcaa caagaagtat ttcctgcagc agccgtatcg gcaacatgat
    37201 ctggcaggaa agattcgtga actgctggac gtgtgagtgg acgagtgcgc cttagtaggt
    37261 ggcggaaaaa ctcgattgtt gcgcaatacg cgatgatcga ctcattcgcg gtgtcggtat
    37321 tgcaatgacc cgcatcctgc tagtagcgcc aggatctggt gcgtccaggc gtgaagaagc
    37381 gcacctccgg tcgtttgaag aaaccgatca agatcatccc ggtaatgaat ccgccgatgt
    37441 gggcaaagaa cgcgacccct cctccctgcg agccgatgct cgcgcctccg ttcaacagct
    37501 gcatcacaaa ccagaatccc agcacgatgc cggccggcac gtaggtcgtg cccacgatcg
    37561 gcgcaagtag caagacgcgg gcatggggaa acagcagcag gtaggcgccc aagaccccgg
    37621 aaattgctcc gctggctccc accatcggca cggtggatgt cgggtcggtc agggcgtgac
    37681 tcaatgccgc caggacgccg cacaccacgt agaacaggat aaatctggca tggcccatga
    37741 tgtcttcaat gttgttgccg aagacccaga ggtacaacat gttgccgatc aggtgcatcc
    37801 aactgccgtg cagaaacatg ctggtcagca tcgtcgcgaa cgcaggcact gccgtgacct
    37861 cgggcgggag catggcgtgg ccgaagacga cggaggggat ggcgccatat tgatacacaa
    37921 aggcttcgcc gggagccggg ggcagcgaac tttggtagag gaacacgagg caacaggcga
    37981 cgatgaaggt gatggtgacg accggtgtgt gttcggtcgg gttatcgtca ttgaggggca
    38041 gcatggcgat ccctatcggg gcggcgcgtt acggcgccgg tcgaggaagt cggggattgg
    38101 aagggagcga gccgtcggct tgcatcggga tggagaaacc ggccgcatgc gtatggcctc
    38161 cgccgccgaa tgatgcggcg atcgagccca catcggttcc ctcctcgcgg gagcgcatgc
    38221 tgtagtagcg tcgtccgttg cggtcatgcc agatgagaca gaatggatgc gcggcggaga
    38281 gccgttcgcc gatctgactg gtaaggactg cgctgtgaac cgcgggcacg gtcgctcctt
    38341 caaactgcac cagggccgcg tgcgaggcca atttcgtgac cagttcgttt tcgtaacgga
    38401 gaatcgcccg gccttcccgc tctaactcct gctgctcgaa gcttgtccag agctggaaat
    38461 cgaacgcata tgaggccagt gcggcgctga tctcccggct gttcgggagc gcccagttcc
    38521 agagatcctt gtcctgaatg tagcgtaaca gccagggggc cggttcatca tgggcccatt
    38581 cccacccgag taccgcaccg gacttctttt gatcgaagta ggcatagggc agatcggcca
    38641 gcgtttgctc ggcggtgatg tgatggtcca ggaccaccag gctcgctgca tctttggcga
    38701 tggcttcgag ggtcggccgg gcgtaactga aatcgacgat gacgatatga tgaccggcca
    38761 ggttggccgg cgggccttcg ccatgtttca cggggcgata ttctgcgttg gggtagcgtc
    38821 gccaaatggc ccaggcggca ccgaacccat ccgcacattc cgcgtgatag agaatcagtg
    38881 taggaggaga agcggcacgg cttcgcatga cggcggcgct cataggctta ccaccttagc
    38941 atgtccgttc atggagacta aagcgcaatc tctggagggg acgcgatgcc ggttggtgcg
    39001 gccggcatcg cgcgggattc cggttatgcc gtcgagggaa gctggttcag gtggttgatc
    39061 agttgccgga ggcgtccggc attccgtcgg ttgaacgaga aggtcaatcg cgcgagatcc
    39121 ttcaaccccg gttcatcgat ttcctcggga agcgacggac gctgctcgaa cgtcccctcc
    39181 gtcagcatat cgctgaattg ctgctgcact tcttcaacct gcgccgcact caacggggtc
    39241 ttcagcctga tggcgagctg gcggcccaca aaccggagcg agtgatagcg ccggtagaac
    39301 gtgcggattt ccgtgacggc atcttcgaca ttgtcgacga tggtgaagag agcgagatct
    39361 tccgcgttga tcaggcgtcg cttcagcaat tggtccgtga tgaaggaatt ccagtgatcc
    39421 cagtagtcgc acccgggcgc ctgaaggcat acgatcggtt tcggatcgct cttgccggtt
    39481 tgcacgaggg tcatgatctc gaacccctcg tcatgggtgc cgaatccgcc gggaaacagc
    39541 gcgatcgcgt gcgactcttt ttggaacatg agcttgcggg tgaagaagta tttgaaggtg
    39601 acgagcttag ggtcatcggc aatgaccgcg ttggcgcctt gctcgaacgg cagcatgata
    39661 ttcacgccga agctatgctc acggccggcg ccttcctgcg acgctcgcat gatgccgtct
    39721 gcgccgccgg tgatggtcat gtagccttct tccaccatcc gtcgggcgaa ttgaaaggcc
    39781 agctggtagt tggggtcgtc cgccggtgtg cgggccgatc cgaacacgct gatcttcagg
    39841 cgctgccggt agccctggaa cactctgaag gcatgccgca gttccttcag cgcccgattc
    39901 acgattttga ggtccaggac atccagctgc gattccgaga gccggatcac acccgcgaga
    39961 atttccttca tgagcgcggc ctggatgtcg tcgtcaggac ggtcgagcag ggtggtgatt
    40021 tgcgcgagaa tctcctcttt ggtcggggtc ggcttggagc gggcggcggg gcttttcatg
    40081 gattgtcacc taacgcacgg taaactcctg acgcgtatcg tcggagagag cactcataaa
    40141 aatcataaca agctgatgcg gatgaagcaa aagattcatg gaacggagct taggagaggc
    40201 cggaggcccg cggcagattt tgcaagaccc aggcgcgaat cttcgactca tcggaggagg
    40261 gaaggggcgc aaattggatg tcgaccccgg gataggtccc gagcgcatcc cggtcttcgt
    40321 ggaattcggg ctcgtaactg ctgttgagaa tggtgccacg cagggagaag ctcaagtctg
    40381 ttcctggaag cataaacgaa aggacgatac tcgtgccggg cagcagcagc gtgcggctct
    40441 cgatgaacgc gccgacgtgg ctcagctgcc ggatcgtggc gaggtgtttc gctccctcgc
    40501 cctcaccatt cgtcacgcgg atggaggcag ggaggcaggt ctcgcatcgt ttgggtgcca
    40561 gggccaggtc tcgcgccgtc aggatgccga ccggctgacc ttgtttcgtc acgatcaata
    40621 gagaggcgcc ggtggaggcc atcacgctgg tggcgtcatc gaggatctga tcgcattcga
    40681 tggaatgcac aggcttcgac atgatcgtgc ggacttccac gtcgtgcggc tccagtccct
    40741 gggcgacgac cttcatcacg atatcggcgg aggtcatgat gccgaacgtg gtctcactat
    40801 ctttcaccag caggcaggga atccgctccc ggtcgaggag caaggcggct tcgctgacag
    40861 agacatcccc ggggacctgt accacgccgg gtgtcatcat gtgggccacc ataggggttg
    40921 agtaatgcgt cggtttgtcg cggagcatgg agccggtttt tatcatcggt taagcctgtt
    40981 tctcgccgtg agaggggctg acgtgtcggc ccggcgacca ccacgtcgaa aggaagtata
    41041 ccagatcgat ttgccgggtt aggttcccca tcttcccttg tcaaccaaga ggttaggcgc
    41101 ttagactgca catgaactat agtcagtatg gttgcttcaa aatatgccaa acgcgatctg
    41161 atgcaaagcg aattcacaga acgtgcgatc ggccttgcca cgcacggtgt agggctgtcg
    41221 gtggatgtct attcgcctga cctgctgcac ctcgtccatt cactgcggga cgcaggcctg
    41281 cagcccgggt atctggaggt cttcaaggcg accacgtcag ccatgcaatg ggcgcggcgg
    41341 cacctgccgg acatgaagct tccttaccat ggcgagggcc tatgggtcac gcagccggat
    41401 tttcctcgga gttcctccgg gacgcaaggg gttgcggagg cctgcgcgca aatcctcgcc
    41461 ctggggagtg cctggctcaa tcacgaatgt gcgatgaagc agatggtggg gtattccttc
    41521 gggacctacc tgccgccgtt gtatacggaa ttgtccgcca ggatgacagc cgagaacctg
    41581 gcctttctac aggaacaggt ggatgagcag gcgcgtcgtc atgggacaga tcctgcgctc
    41641 gtcctcttgg aaatgccgcc gctcacgtat tttggttgcg gctctctcgc gattcccgcc
    41701 ttcttccggg ccgtgaccga ccgcgtggcc tgcggtctgg tgttggacat cgggcatctg
    41761 tggacggtgt atcgttacac gggggcctgg ctgcgtcaga cgttggaggc ctttgccgct
    41821 gaatttttag acgccttcc
//
LOCUS       DAORRQ010000041        39452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_66_length_39452_cov_187.371373, whole genome shotgun sequence.
ACCESSION   DAORRQ010000041 DAORRQ010000000
VERSION     DAORRQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 39452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39452
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66_length_39452_cov_187.371373"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..2296)
                     /gene="tssI"
                     /locus_tag="PK224_13450"
     CDS             complement(71..2296)
                     /gene="tssI"
                     /locus_tag="PK224_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HRB16795.1"
                     /translation="MPHTQENRLIGIETPLGKDILLLRGFSGQEGISRLSNFDLDLLS
                     HDPDIKFEDIIGKRVTLRVTLGSEKKRYFNGFISRFSQTGSDRGLANYRATMVPWLWF
                     LTRTSDCRIFQKKTIPDIIEQIFKDLGFTDYKLQLQGSFEPRDYCVQYRETDFNFVSR
                     LMEQYGLFYFFEHEQDKHTLVIANDPSAHQPCPEQNAAKWVAQGSGALAEDVITSMQW
                     EQTLRPGKYAVTDYNFETPDTSLHAEMKTVIEVGGNSKFEIYDFPGEYSKKNQGDTIA
                     KIRMEEEEAQYLVVTGTSSCRAFTSGYRFDLKDYVRKDMNQAYVLTNMQHVATVGSTY
                     TTATVGKDESAYSNSFTCIPHKVPFRPPQMTPKPIIQGTQTAVVTGKAGEEIWTDNYG
                     RVKVQFHWDREGQFDEFSSPWIRVSQVHAGKGFGGVDIPRIGEEVIVGFLEGDADQPI
                     IVGRVYNAKNMPPNGLPGAGMVSGLKSNSTPGGGGNNSIMMNDTKGNELYSMNAQFNC
                     TENVGNDRTTTIKVNDSLTVNNNQTIGIAVDRKETVGGTETITVTGHRTETVNGGETV
                     TVTGGRSHTVNGVETTTISLAEVHSVGAGRMHNVGAAEAITVGGAQMVNVGAAQMVNV
                     GGLQSLNVGASKSTKAGGNISEKAGGNISETAAGKISNTAGGAMTLTATGDFAAKTSA
                     KGSVDAASELVLKCGGASIVLKSGGEITIKGTDITINGSKIGVKGSGPVTLKGSKVTN
                     N"
     gene            complement(2528..5233)
                     /gene="tssH"
                     /locus_tag="PK224_13455"
     CDS             complement(2528..5233)
                     /gene="tssH"
                     /locus_tag="PK224_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HRB16796.1"
                     /translation="MNINLKGLIGKLDDTCRRALEGAAGLCLSRTHYDVDIEHLLTKV
                     LEMSNTDVHKICRHYGIDQSRLARDITRTLDRFKTGNARTPTLAPRVPRLINEAWSLA
                     SIEYGVNRVRSGHVMLALLAEDDFARMMREMSPEVQKISAEDLHAQLPKIVSGSAEDL
                     EEVQAGGVETPTAGGAPHASRTPAIDQFTIDLTARAKQGQVDPVLGRDAEIRQVIDIL
                     TRRRQNNPILTGEAGVGKTAVVEGLALRIVAGDVPPPLQNVTVRVLDLALLQAGAGVK
                     GEFESRLKSVINEVKGSPQPIITFIDEAHTLIGAGGAAGQGDAANILKPALARGELRT
                     IAATTWAEYKKYFEKDPALTRRFQVVKVEEPTESVAISMMRGLAGTLEEHHKVRLLDE
                     AIEAAVRLSHRYISGRQLPDKAVSVLDTACARVALGQVAEPPALEDARRRISLINTEI
                     DILEREDITGGNHQDRVAELGREKAAEEKRQAELKIRWEQEKKLVGEIAEIRQKLEQQ
                     AAPPKAPEKGTDKSADKATEKPPAKLTAQEVETLQADLTRLKKELTALQGENPLLQPS
                     VDGQAIAQVISAWTGIPIGRMVKDEINTVLSLKEHLEKRVVGQSHALDALSQRLRTAR
                     AKLEDPRRPIGVFMFVGPSGVGKTETGLALAELLFGSDQNITTLNMSEFKEEHKVSLL
                     MGSPPGYVGYGEGGVLTEAVRRRPYSVILLDEMEKAHPGVQDIFYQVFDKGMMKDGEG
                     RDIDFKNTVIIMTSNAGTDTVMKLCADPETRPEPEALADAIRPDLLKYFKPAFLGRLI
                     VVPFFPISPDIMRRIIGLQLSRIMRRIKENHRAAMSYDESLVTAIADRCTEVESGARN
                     VDHILTRTLLPELSTEFLSRMAAGESVNKVHISVEPAGNFRYEVA"
     gene            complement(5257..6321)
                     /gene="tssG"
                     /locus_tag="PK224_13460"
     CDS             complement(5257..6321)
                     /gene="tssG"
                     /locus_tag="PK224_13460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HRB16797.1"
                     /translation="MATQSRGTDPGLTQDLTQRGFRFDFFQAVRLLARVYPDRQPVGE
                     SATPSKEIVRFRAHQSLAFPPSAIHQINQARDERRPAEMTVAFMGLTGPQGVLPRYYT
                     ELMLERLQAKDQTLRDFFDVFNHRMISLFFRAWEKHHCTAGFEQWLLKGSDDRFARCL
                     FAVAGLGTTGLRDRLTIDDRSVLRYVGLLGQRPRSADALERCLADYFQVPVRVNQFIG
                     AWLPLEQADWTTIGVTGNNNILGRSALAGTKIWDQQAAFKVELGPLKFEQFDRLLPSG
                     RAYPTLVQLTKLFAGPELDFDVQLRLRAEEVPPTRLRSTDTYAPRLGWTTWLKTKEFD
                     HHADDVQFSGSTMMARAGRA"
     gene            complement(6285..8099)
                     /gene="tssF"
                     /locus_tag="PK224_13465"
     CDS             complement(6285..8099)
                     /gene="tssF"
                     /locus_tag="PK224_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HRB16798.1"
                     /translation="MQETLLEYYERELSTLRQLGQEFAQAYPKVAGRLLLEPNKCEDP
                     HVERLIQAFAFLAARVHLKIDDEFPEITDALLGMLYPHYLAPIPSMSIVEFVLDPEQG
                     KLTSGYVMPVGTQLFSRPIDGTQCRFRTCYSTTLWPIMLASVKVQPAERALPGGKPGA
                     VIKLELHAQGGLTFKDLQVEQLRFYLNGEAPLAYSLYELLLNHTHEVRCRAGGGKPGE
                     KPVLLPSGSLRPVGFGMDEGLLPYPSQALPGYRLLHEYFTFPQKFLFIEIANLQHVVK
                     AGIGSKVEIEIVLDQPPRADYMPTTDTFRLGCAPVVNLFTQTAEPIRLDQTQHEYRVI
                     PEVRRPLANEIYSIDAVSLLSGDKQTVRPVQPIYSLKHTRLDREQSAFWYQSRRPSDR
                     KGDVGSEVYLSLVDLEMDPTVPAGDTLMISTTCTNRGLTRRMAVDDIRGDFELEKAAP
                     VERIRALVKPTEPMQPPLGGETQWRLISHLSLNYLSLTEGSPQALQEILRLYDFSDSS
                     VVHQQIEGITAVSSRRVTRRPTSLAWNGFCRGMEVTLEFDENKYVGSSVYLFASVLEK
                     FFGLYASLNSFTQLVARTQQRERPLKQWPPRAGEQILV"
     gene            complement(8099..8911)
                     /gene="tssE"
                     /locus_tag="PK224_13470"
     CDS             complement(8099..8911)
                     /gene="tssE"
                     /locus_tag="PK224_13470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HRB16799.1"
                     /translation="MPSVLDRLLDDDPRAGDPHLDTLDIADPAKLTGRLRSPQDSVTR
                     FLREQFRPEERELVDREGSGAGTNDLLLAITEGLNRIIRGPLLYERKRFEGIKLSPDT
                     VRLMEDSRKTVHVALLNRMLLDEIFADELHKMRKPAPSSANIRELKKSISRDIEALLN
                     TRRELLEGAPVEYTEVNNSLLMFGLPDFTSYSLLNPEQRKLIRRCVEEALTKFEPRLK
                     SVRVTLEPPRKFDAALHFRIDALLRLDPVPEAVSFDAALQLGTSSYSVRGEA"
     gene            complement(9027..9482)
                     /locus_tag="PK224_13475"
     CDS             complement(9027..9482)
                     /locus_tag="PK224_13475"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HRB16800.1"
                     /translation="MAEVRQENAFLKLGDIKGKATTDKFKDQIVFQSMSYGVNQAGEW
                     EEGDRLSGNITTFADLVIVKEMDAASPSLAKACAVKEQIPKAEISLVSGKDVYCKVTL
                     DDVIVSNVSVGFHSAEARPTETIALRYRAAMWEWGTSKGGYDLRKGVKK"
     gene            complement(9963..11456)
                     /gene="tssC"
                     /locus_tag="PK224_13480"
     CDS             complement(9963..11456)
                     /gene="tssC"
                     /locus_tag="PK224_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HRB16801.1"
                     /translation="MAEEAKQAQPQGAAAAGTEESGVLDQIINETRIGKDDWERDQSR
                     RQIATLVEEVMKGTLRVSKDLEATINARIADIDKVLSAQLNAIMHSPEFQKLEGAWRG
                     LHYFVMQSETSTMLKIRVLNISKDELRKDLEKAVEFDQSALFKKVYEEEYGTFGGAPY
                     GALIGDFEFGRHPQDLALLEKVSNVAAAAHAPFISAAAPGLLGLEGFTDLPNPRDLAK
                     KFETPDYAKWKSFRESEDSRYVGLTLPHVLMRLPYGPDTVPVEAFNFKEDVDGTDHSK
                     YLWGNAAYAMGARLTDAFAKYGWTAAIRGVEGGGRVDGLPTHTFRTDEGEIALKCPTE
                     IAITDRREKELSDLGFIPLVHCKGTDFAAFFGAQSCQKAKKYDTDAANANARLSTQLQ
                     YLLAMSRFAHYLKSIMRDKIGSFMTRKDCEDFLNRWISKYVVSTEDAGQEIKAKFPLK
                     EARVEVAEVAGKPGVYKAVAFLKPHFQLDELTVSLRLVAELPPPAKR"
     gene            complement(11459..11992)
                     /gene="tssB"
                     /locus_tag="PK224_13485"
     CDS             complement(11459..11992)
                     /gene="tssB"
                     /locus_tag="PK224_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HRB16802.1"
                     /translation="MARESTQHKLDRVRPPRVQITYDVETGGAIEMKELPFVVGVMAD
                     LAGKPEQPPALLKDPKRKFVETDRDNFNDVMKGIGPRLAYKVDNKLTNDDTKIGVELR
                     FNNIDDFSPEQVAEQVEPLKKLVEVRKQLSALLAKTDGNDKLAEKLQEIIANSELQQK
                     IGKEAGLGTEGGSQKEG"
     gene            complement(12016..13134)
                     /gene="tssA"
                     /locus_tag="PK224_13490"
     CDS             complement(12016..13134)
                     /gene="tssA"
                     /locus_tag="PK224_13490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HRB16803.1"
                     /translation="MASVPTVDIDAILKPISGDKPCGVDPRDGVSFELLKEARREEDA
                     ASQGDWKREVKVADWPKAIQLATKILSTEGKDLQVAAWLTEGLVRKHGSAGLRDGLKI
                     LRGLHEQYWDSFYPSIEDGDLEFRGGRLEALNKILPVAILNMPLVHPPGGPAYSCWQY
                     KESQEVENLRRGAATDGERKRQLAEALEEGKLEGEKFDKAVAATPLSHCSTILENLNQ
                     SWDEFEQFERILDEKYRPEAPSLRLLKEALSECRSLMNSIVRKKGGVGAPTVNQPDIA
                     PREADMAPVVQMTASGSGIMPVDRTDALRRLGAVAEFFLRTEPHSPVAYLVQRAARWG
                     EMPLEEWLQEVIKSEDVLGNVRETLGLTQSRPPSDQKS"
     gene            complement(13147..13950)
                     /locus_tag="PK224_13495"
     CDS             complement(13147..13950)
                     /locus_tag="PK224_13495"
                     /inference="COORDINATES: protein motif:HMM:NF018699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HRB16804.1"
                     /translation="MGANELLKQHQLSAAISELNEDIKRHPIDARLRTFLFELLCFTG
                     DYERAQRQLDVIGHQNEKVGIGVEVYKNMLRAEVTRRRFFKEGIKPSFLFEPPEHVAL
                     YVDAHNQLREAHPVEAKALYERAQRARPEIRGRMKGQPFTSFRDSDDRLRGILEVIVR
                     DSYIWIPFERIHKVVIPQPKQLRDLLWVPATVEIEDGPVGEAFLPALYAGSELESDDK
                     VRLGRMTEWLDLGAGLVGGVGQKTFLVDDREVSILEVEELEFEVLAAVQ"
     gene            complement(14092..15048)
                     /gene="tagF"
                     /locus_tag="PK224_13500"
     CDS             complement(14092..15048)
                     /gene="tagF"
                     /locus_tag="PK224_13500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HRB16805.1"
                     /translation="MYSSTLGYYGKLPLSAEFIRCQASGAEIDELDQWIREGMFHAKS
                     TLGPAWSMDFLQGDQWAFLYLPPDRSRFLAGLLKPSQDKAGREFPFLIYLLLERKEFH
                     ELPWCAPMHFREFFGQSHRLLEDVSTETDLNRLQFRLQALSPVEVPESTSIETRYHVE
                     LLHRRMRDHWADVFGGFDCTQKYALLRSLLHSRSGSDSASRGHWQAQFPLLIESKEET
                     YDLPFWMDLASHASGHRPEAGILFWNRWPSKVKPLLLVSLERPLPQLLLSLIRPEGRP
                     NELLENDDAGHLAEAGRALLDDGDVTLEAFLHRVSGLRAEGS"
     gene            complement(15054..18686)
                     /gene="tssM"
                     /locus_tag="PK224_13505"
     CDS             complement(15054..18686)
                     /gene="tssM"
                     /locus_tag="PK224_13505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HRB16806.1"
                     /translation="MGLFARIKSLLVAMLGHRRLVVEFGLVCLICLVWLAGPTIGLES
                     VESRVQVIIAIVLLRFIFSVLQHILSQRRAVQLEASLQQQAQVQVAAARPDHKEELEA
                     LRRQFDKGLAALKESTIGKGVKGKAALYALPWYMFIGPPASGKSTALRHSGLQFPYLG
                     ESGQGLQGMGGTRNCDWWFTNDAVLLDTAGRYVTHEEDQKEWLGFLDLLKKCRKEKPL
                     NGILVAISIADLMQASEEELEAHAKKIRSRIDELMTHLGITFPIYLIFTKCDLVRGFV
                     EFFDELTKAERERIWGCTFPKSAAKEPAQVRFKTEFDQLLTAVKSRRLARLVNARGPY
                     KLNIFTFPLQLASATDKLAHFVEVLFQSNPYQDNPMFRGFYLTSGTQEGAPIDRILGA
                     ISRASGLGDMVSSMFSPTETKSYFLKDLFTDVIFPDQFLAGPSTAIHKQRGFLRVAAF
                     AAGTLFVAISVIALAFSYIGNRALVSNTLSVALNAPDIAVTDAASLEKNVEYLDRVGD
                     RLGELVSYSENGVPFRLWGLYRGERLLGDLEEVYARQFEKILLIPTKRAMEVELDQFA
                     SGESRGEAIGASDYYYSMLKAYIMLGEPKRLSSPYLERWLTAHWNDRLKGLYATYAVP
                     PLVQASIQRHMALYTGVLARAQQGRVELNKHLVAGAQERLRDIPLVDRLYGLSLREID
                     ESLRPAPFSVETALQGSHHGTVVSDHLIPGAFTYEGWKGPFQIAMGRVLEGLGSEAWV
                     VGEPDTKQIDLERGIKTLYFQDYRLHWRAFLKSLSLGPAVTPTDMEKLLSTLSQTDSP
                     FLRILEAVDRNTVPEPEGIAKLQESAVGLLGKVKEKLGLETVGKKLEKPPRAPDAAEF
                     PGGVTVHFLALHDLIAARKDAKEEAPIIQYLTELRKGHEAFRPILLEETINPNTKILA
                     RTIVAGGSNELKLGMEKTGFLLKKLDAELRESMGALLSEPWLMTMRGVLERTRLDIDQ
                     RWGTDVFQACQRAIERKYPFRSSGEDAVVEDVVNFLHPDKGALWRFYQADLSPFLEER
                     GDHLVRRAWSGVALTVSDAFIESLEKARFVSESLFTKASPDLGSVIEVYPYPPGGAGG
                     RSVSEIRLDIGGEPLRYRMEPQDWWEMKWPGPTPSLGAALVVKVGDNSVTRDFKDVWG
                     LFKLIRAGVIEATDHSDVKVKVQWELQTPDFKPLQIRYDVRAKSAKNPFRPGFFEQFT
                     CVDHVT"
     gene            complement(18686..19384)
                     /gene="icmH"
                     /locus_tag="PK224_13510"
     CDS             complement(18686..19384)
                     /gene="icmH"
                     /locus_tag="PK224_13510"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HRB16807.1"
                     /translation="MVTVQAEKTNSLSEVFNDLLVLGVQLRDATNPGGVESLRTRLQQ
                     LFQSAEERGRTSGFPQEALTQARYAVAAFLDEMLINSRWPQKDEWAARPLQYELFGEF
                     TAGEGFFKRLENIRGGIPMNADLLELYTYCLMLGFEGQYKLQDREKLRGLVEDLSRQI
                     QGKRGEIPPLSPRGRRPEELLEMVKSELPVWVIIATSTSLVLLFYLGLSLLIGQDAGY
                     VGHELKRLLQEVRG"
     gene            complement(19391..20731)
                     /gene="tssK"
                     /locus_tag="PK224_13515"
     CDS             complement(19391..20731)
                     /gene="tssK"
                     /locus_tag="PK224_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HRB16808.1"
                     /translation="MENNQRVVWSEGMLLTPHLFQQWDQYYERHLNERLRALVAFGYG
                     VLRLDFDHDGLSNGRVTLLGFHGLLPDGVVVRIPEEDVAPQTRLIGDLFPASVDHLDV
                     FLALPVEQMDGVNCVLGDESGSRAARYSSAYVERKDSTTGGNPQEILVARKNLRILFS
                     GEPTSDMVVLKIGELLRTQSGAVALKETYIPACLWISASPYLMRLLRGLIELLTAQIG
                     SYGNAQRGILEAIGMDLGKYHLLSALMAHLPAMQHVNLVEKVHPEFLYLILARLTGAL
                     SMLAGPLEVLALPPYRHDNLAATFEDLDHTIRSIVERFQPTHYLTIALEASGENVWVA
                     RVPESRLFASSQFFLVVAGDLSEDQIRQQVPQFIRIGSPAKLKEIVAAAMPGVRLYHT
                     PRPPATLPVRVGQQYFRLDDRGVFWEEIKKTQIVALHVPQSLQSLRLQLLATKE"
     gene            complement(20752..21246)
                     /gene="tssJ"
                     /locus_tag="PK224_13520"
     CDS             complement(20752..21246)
                     /gene="tssJ"
                     /locus_tag="PK224_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HRB16809.1"
                     /translation="MSKRLNVVGNLCVFLIILMVGGITAGCGSGIKDVRVTLLPTYRL
                     NPDESGAPLSVVVRVYQLRNRERFDRADFRALWRNDEKELAGDVLERREVTVYPETKT
                     SIELQVDQKKGVQFLGVMALFRKPEGELWRRVVSSDQSTWIPFTTPTVKLILDEHKLK
                     LKDE"
     gene            complement(21218..22474)
                     /gene="tagH"
                     /locus_tag="PK224_13525"
     CDS             complement(21218..22474)
                     /gene="tagH"
                     /locus_tag="PK224_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HRB16810.1"
                     /translation="MAIRLEIHPRKDLGDGKSWEHIFDQGEVTIGRASSNDVTLQDPQ
                     RVVSSRHAEIRRKANACVLVDMGSTNGTRMNEQSLVPQREYPLQEGDRIVIGDFTILF
                     SVQTPQTNGMAHNVVEEPQTAVQGVDADALVYELCRRYAELQKCTTQEREAELLEMLR
                     RALAQQVGSVCESLMGKIRAGLCLRLGQPAAAPLPAEAAPAQPVRTDQAAGEAAYRGL
                     LAIAQKYCLRLPSPTSAEFIGQFARRIDQVLQITFGNLVESLRGRRQFARELEVEATR
                     ILNWAPNQIKQAENEQQVGAYLFDALANPKDAEAVMADLQRVFRDLALHHIGVIKGFE
                     ESLRAVLREFDPATIEAEFEVSPVEIGPLRLPPSFRLLKQWGAWKHFKRKHRRFTEEE
                     VRIFEQILAPHFAKGYLDVQKTQRGR"
     gene            complement(22483..23547)
                     /locus_tag="PK224_13530"
     CDS             complement(22483..23547)
                     /locus_tag="PK224_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16811.1"
                     /translation="MAEFYRLYQRPYLSLDPAHISGRVSDHSFTWLPARWLFAVELDV
                     AKGAQSLVHETMPKEMARDIFVPSGDLDADYAAPVMRQWPMGRELPVTVLLRSIERIP
                     DDTDEQLSRGLLRAHVFSETVSFVDCSEQDTFHLAIRLPGSGTAPVPLWARRVEKAER
                     GLVVSGMTDPIPNATWTQLERAKQQVFSDTGASIYRAFHHASDLYSLGMLLFRGLLVH
                     QTQSLEQVQQHIAGVLERLEPLVQGLNPDDHWTLFTRVSGRLKERSEVFLPPSISIPE
                     SLWYEALICGLRLVPGIRGFSFCGSEGSTDHTPLPEALQGAMCAAECVAEQARVELFD
                     AAARHRDLMRVCDRALAERM"
     gene            24156..24944
                     /locus_tag="PK224_13535"
     CDS             24156..24944
                     /locus_tag="PK224_13535"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRB16812.1"
                     /translation="MLPALESSTPRWHGAGLSDVGLLRSSNQDAFVVDNNLGLWVVAD
                     GMGGRAGGNIASSLTVKALLDYFHHSKDSGESAIFKRGDGEASVQLRQAIRAANEAVR
                     QRALEEPALDGMGTTIVAVLVSSLSPLQLVIGHVGDSRAYLYRGQELRLLTRDHSLVE
                     DLLTRGQISPEEVGTHPQRHILVRALGIEGQTDPDISNQAFQSGDILLLCTDGITTML
                     SDRHIIAQLTRSADSPDEACRGLISEANARGGKDNSTAVVVRFS"
     gene            25021..25998
                     /locus_tag="PK224_13540"
     CDS             25021..25998
                     /locus_tag="PK224_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRB16813.1"
                     /translation="MSLDFESLSLTEIIRLQNQLSALLARRFEKTHAPAFSDVTGSTA
                     YFARFGNEAGRRLQQRHVDLIAKSLQGSGGRIVDTAGDGVFLCFPNVEAAVEVLERFQ
                     TLRLQENAHLPPEHQMTTRIGIHWGTVLTDGVIVTGDPVNLCAKLAATAQPGEIRITK
                     PAFLELTGQRRLRCRSIGPVKLAGVQEPMDMFVFAWADPVRFPSAVLIAETGEQIPLP
                     PQPVITFGRLREMNGTHANDIVLTHKDPTRTQHISRWHFEVRRAPEGLILRSVSTQPT
                     EVNGRVLCKGEETSISAGTIVRLADVLTLRFIAGASDQASEDNAVTTVS"
     gene            complement(26055..26999)
                     /locus_tag="PK224_13545"
     CDS             complement(26055..26999)
                     /locus_tag="PK224_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRB16814.1"
                     /translation="MNKLRPWFYALLTLCVLLSLFNPQHVIAETKAGSHELETVRGTG
                     CYSYGDNETPAQAKRAAMVIAQEQAVRSHRVFVESTSTVRKFQLEDDLIQTVSAGMLE
                     QIRVENEEKKGQEICLTVTAKLSPVSFEDLIKQRANAKHVAQAAQTPLLPQSQGFGLR
                     VWVDKADGHYIEGDRLVVYVQSERDAYLKLDYFMADGCVVHLVPNVYRGQALIHGGQT
                     YSFGGDADPEHILITSPFGAETIKAMLSAVPIEESGYDAGRGCDESRDYLQRLESGIK
                     KGSRGAKLTGVDRSVPLVTTSKAVDTYTKEKPGPPRRK"
     gene            27290..29842
                     /locus_tag="PK224_13550"
     CDS             27290..29842
                     /locus_tag="PK224_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRB16815.1"
                     /translation="MAKVLEPPTTVQNRLLPPPFHSQASYVIPEMKTMTLLAVLFVCI
                     AVAGQAWTTDTLAQEPVTPEKLMEQGQSAYQQGAFTQAMQHWTEAGRRYERDGKTREQ
                     IKAQVNLTQALYQTGHYKEAGAILVDSGKLADKIGDRLLKAIVQGRLGSILFALGDNE
                     PALRAIEEGLASARALDNSALAATMLNDQGNILTADGRYSSAVAAYTESSILAKASHQ
                     PALTTIALINAGRATIQEGSLDTAKARLDIAAEEISSMPDSFDKANAWLSLGDAYEEI
                     VQPRIGNVSSRNVVQRTLLAAKSSRGVELQPGTPSTKEPAATTPPSDTQPSQKKPSPL
                     PAPPILRQAAEAYWNAIQVATKIGDSRNESYGWGYLGHLYEMQHRTDEALDLTRRALL
                     SAQQVSSPESQYRWHWQTARLLRTKGQINDAIAAYQRAIETLQPIRSEFMVGGQNRRF
                     SFRETTGNLFFELSDLLLQRASSTPDLAARQHLLGQAQDTVELYKAAELQDYFRDECV
                     ATARSTSTAVAQESKSTAIVYPIILADRMELLVSMPAGLKQFIVPVTGEQLTEEVRAF
                     RLGLEDRSNNAYRPHAQKLYQWLIRPMEADLTTAHITTLVFVPDGPLRTIPMGPLHDG
                     TKFLIERYAVATTPGLTLTDPRPLNRKQIRLFSMGLTEAVQGFPALPYVGNELKAVQA
                     IYGGKQLLNEEFRAGKVERDLNEQPYNMIHIASHGKVESDVTKSFVLTFDDRITMDRL
                     SHLVGLFEMRTVPLELLTLSACETAAGDDRAALGLAGMAIKAGAKSALATLWFIDDEA
                     TAELIAEFYKNLQDPATSKAMALQQAQLTLLKNPDRAHPSLWAPFLLINNWL"
     gene            30041..30865
                     /locus_tag="PK224_13555"
     CDS             30041..30865
                     /locus_tag="PK224_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16816.1"
                     /translation="MTSPFPIAMPAFRITILIGLFVLSNSISEARFSLGAETLTGLSS
                     TTTSSTWPSLTYHSPRERPHANVKGEEFGYFPRVEAEPLVATVEDSPITGARAGPVTL
                     ARTQPPLHSGTRTTVLGDLYLADDHPIVAAEAAELLASTALFLDRDPIAHLTLEAYCD
                     ERGTEAYSFLIGREWVLAVERRLQEMRVEDRRVTTVSYGLQQPACQEASTTCREDNLR
                     MKWSIRLLSPVTSQQGCLIRLKLATNTLLDQNALLVANQPYLRRIQQGEPYSAKAP"
     gene            complement(31140..32993)
                     /locus_tag="PK224_13560"
     CDS             complement(31140..32993)
                     /locus_tag="PK224_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HRB16817.1"
                     /translation="MRCRIDHGEENGPGRHVAESRRIAPSGSNFAPTWLSSSWRGVPF
                     SLLLVVACGLSLPDSGYPQVGLPPVIDPSGRSGLPPPVEQKKPLRPQEPPADILPPVQ
                     PSPRELGEKGPVLRVFVRQIQVTGSTVLSPEELAQLTAPYENREVTTEDLEELRRQIT
                     LAYIDKGYPNSGAVLPDQAVVDGMITMQVVEGRLSEVRIQGAKWFRPSYLRDRIELSA
                     GPPFNMNPLRDRLQILLQDDRLERLNAELKPGAVPGEAVLDVAVQEANPVRAFVEYNN
                     YINPTVGENQLRGTVAHRNLTGRGDVLSMSFGASGQASPVKIGVFPAIDAGYAIPLNR
                     YDTTFFAGYRFFKFKVVEDPFRPLDIKSETQIFTLSLRQPVYRTLNDEVTIALVGEYE
                     QNANSLLGTPFDFVNGMKNGFGNVAALRFIQEWTHRTTESVFSVRSRFSAGVDVLGAT
                     VNGAPGAADGQFFSWLGQAEWLKRFESRIEILNFLNMQLANDRLFPLEQMAVGGRYSV
                     RGYRENTLLRDNAIVYQFETRFPVWSSNEGFPYVQFCPFADVGHSWSAKGVSGDPQTL
                     ASVGAGLRFNITTVANLNVYWGRRLVTNNVSNPHNGLQDEGVHIQFVLNVL"
     gene            complement(33003..36950)
                     /locus_tag="PK224_13565"
     CDS             complement(33003..36950)
                     /locus_tag="PK224_13565"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HRB16818.1"
                     /translation="MAQNHSSWRAVCLLSFLLGLAPLLWATVSEGQTTSITSSGLGTT
                     ISPASIDPAGRPNYIITGGTRPGDGPNLFHSFGDFSVGTNNVARFFNEAGRSTTNILS
                     RVTGGQTSNIYGTIQTAGFGTAALWLINPAGIVFGPSASLNVGGSVHFSTADYLRLGS
                     GNDRFYADLGKTSQLTSAAVTAFGFLGERPAGPIIVQAGTSLSVSDGKTISLVGGDIS
                     ITGRTVSAPGGQINLASVAGAGEVIPNQLGETPSLGLANVSKLGTIQLTEGAILRTSS
                     NTGDAGPVLIRSGRFVMENAGIEAITTAPSPGPFVPQLSGGIVDILSNTIVIKGGAVQ
                     TELGFGTTFPVFARDPGTSKASGLSILTNNQGDITLASSDVTLDNAKIVSYGQQVVSS
                     LAGSATGQIQIRGMQGAQSLADQVTISNKSLLHTAGTASDCDCIVLGRDVSIQTNNLD
                     IADSRLSTTQGIVSVFAENELFSRGGNTFEKDGSVQNLRANQNDIELTAGKRISLGQG
                     DFIAGKLGGGLFALPIKGDVVIKAPVVSMSGTTVEANGDTIVRGGDIVIRADETEITG
                     SRFSTNGLGGTPVPGSGTAGSIKITGMQDGVKAETITLVKSTVESLVTQAGSNLGVFG
                     NSGSIILEGNNVLLDGSTVSVTHGGAGQSGSIIVNVGSLTLTNGSNLTSAASGKDFSS
                     GSTLIAAAPGGPISIQGNEVQLIKGSGINASSTGDGSAGTVTIRGTNGPGSAENLVTL
                     DNSTISTTISGGSPVTVPATIDLAGEVIRLANGSRITADTSGEASAGNIHLDTADFAA
                     SNGAMITSSSTGTGTSAGSAGQILIGGILSGPATNISLDNSSISTTISGGTLTTSRAN
                     IDLSAQTIRLVNGARITADTSGDASAGDITLHAANVATSNGAKISSSSTSTGTSAGPA
                     GGIVIEGPGESFAESVSLDNSSLSIILRGGDSEPIRGRGHISILARSISLENGTHIEA
                     DTSGGAFAGAILFQGSGSFAVSKGARITSTSTGTGHAGDILVGSGNVVIENSTFSTSA
                     TQASAGSIRLVAPSMVGLTNAQLTSTAESGKGGTIAIQSGDIAINNGSLVSTITTGAN
                     DAGNITLTSGTNIFIANSTVSTSAAKASGGSIDLKAPNLVRLVGANLTSSVAGLAGSS
                     GGNITIDTAHPQFVIMQGNSQILAKANEGQGGAITIIGGVVLQEPGSVLDATAGPAGI
                     SGSINIQAPFQQLAGAIAPLPQAFAVATNLYGQRCATEKGGQFSSFVQGARDGVPPQP
                     GDLIPSPLLLEPDEAASNIRSQAASSLSAIRLGLPGFDRSSRSSLTVFAGCRS"
     gene            38400..39452
                     /locus_tag="PK224_13570"
     CDS             38400..39452
                     /locus_tag="PK224_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HRB16819.1"
                     /translation="MKLTKSERLELQRQARASNGRADSARHARLILLLADGLTWATIR
                     AKLDCNDSYISRWSQRFAADRLSGLFARYAGRARYKVTDRIEARVLAWTTKHAPDDGS
                     THWSSRKLAAELGGISHMTVTRIWAKHALRPHRLEGYLASNDPDFEAKAADVIGLYLH
                     PPQHAAVFCVDEKTAIQALDRKDPVLPLSPGRAERHGFEYVRHGTLSLYAAFNTKTGE
                     VLGKTAPRHTSAEFVAFLTDIVTNQPNGKEIHVIADNLSAHKTARVEAFLAQYPRVHL
                     HFTPTYSSWLNQVELWFAKIERDVIARGVFTSVPDLKRKLMRYIRHYNTQPKPVKWKY
                     FDASRRITPESIVTVH"
BASE COUNT         8463 a        11913 c        10412 g         8664 t
ORIGIN      
        1 ccacagaaaa cccaggtaag tcatctctcc cggttctttt tagcatcgtt ctctgtccta
       61 ccattcgaag ctagttgttg gtgacctttg atcccttgag cgtgaccggc cctgaaccct
      121 ttacaccgat tttgcttccg ttaattgtga tatctgttcc cttgattgtg atttcccctc
      181 cacttttcaa gacaatcgac gcccctccac atttcagaac caactcactc gccgcatcga
      241 ctgaaccttt agcgctagtc tttgccgcaa agtctcccgt tgctgtcagg gtcattgctc
      301 cgcccgctgt attggaaatt tttccagccg ctgtttccga tatattccct ccagccttct
      361 cagatatgtt gccacctgct ttcgtggact tgctcgcccc gacatttaag ctttgcaatc
      421 cacccacatt gaccatttgg gcagcaccta cattgaccat ttgggcacca ccgacggtga
      481 tcgcctccgc cgctccaaca ttatgcatac gacctgcgcc gaccgaatgg acttccgcaa
      541 gtgaaatggt cgtggtttct accccattca ctgtgtggct tcgcccacca gtcactgtga
      601 ctgtctcgcc accattaact gtttcagtgc gatgtccggt gacggtaatg gtttcggttc
      661 ctccgacggt ttctttccga tcgacagcta tgccaatcgt ctggttattg ttcaccgtca
      721 gcgagtcgtt aaccttgatg gtggtcgttc tgtcgtttcc tacattctcc gtacagttaa
      781 attgggcgtt catgctgtac agttcgttgc ccttggtatc attcatcatg atactgttgt
      841 tcccaccacc ccccggtgtg ctgttactct taagcccgct aaccatgccc gcgccgggca
      901 atccgttcgg aggcatattc ttggcgttgt agacacgccc gacaataata ggttgatcgg
      961 cgtctccttc cagaaagccc actatcacct cttcgccgat ccttgggatg tcgacgcccc
     1021 cgaaaccttt tcccgcatgc acctgcgaga cgcgaatcca gggagagcta aactcatcga
     1081 attgtccttc ccgatcccag tggaactgga ccttcacccg cccatagtta tcggtccaga
     1141 tctcctcacc cgcttttcca gtcacaacag ccgtctgcgt tccttgaata atcggcttgg
     1201 gtgtcatctg aggtggtcga aacgggacct tgtggggaat gcaggtaaaa gaattgctgt
     1261 aggccgattc atccttacca acggtggccg tcgtataggt gctgccgacc gtggcgacat
     1321 gctgcatgtt ggtcaggaca taggcctgat tcatgtcttt gcgtacatag tctttcaagt
     1381 cgaagcgata gccggacgta aacgcccggc aggagctggt gccggtcacg acgagatact
     1441 gggcttcctc ttcctccatt cggatcttcg cgatcgtatc accctgattt ttcttactgt
     1501 attctccagg aaaatcgtag atctcaaact tgctgtttcc ccccacctcg atgacggttt
     1561 tcatctcagc atgaaggctg gtgtcgggtg tctcaaagtt ataatcggta acggcatact
     1621 tgccgggccg caaggtctgc tcccattgca tactcgtaat gacatcttcc gccagcgcac
     1681 cgctgccctg cgcgacccat ttggctgcgt tctgctccgg acagggctga tgcgccgacg
     1741 ggtcgttggc aatgactaat gtgtgcttat cctgttcatg ttcaaagaag tagaacaggc
     1801 cgtactgttc catcagacgc gacacgaaat taaaatcggt ttcccgatac tggacgcagt
     1861 agtcgcgcgg ttcgaagctg ccttgcaact gcagcttata atccgtgaat cctagatctt
     1921 taaaaatttg ctcgatgata tccggaatgg ttttcttctg gaagattcga caatcggacg
     1981 tccgcgtgag gaaccagagc cagggcacca tggtggcccg atagttagcc aggccccggt
     2041 cgctcccggt ctggctgaag cggctgataa agccgttgaa gtatcgtttc ttctccgagc
     2101 cgagtgtgac gcgaagcgtg acgcgcttgc cgatgatgtc ctcgaatttg atatcggggt
     2161 cgtgcgagag caggtccaaa tcgaaattcg agagacgcga aatcccctcc tgcccggaaa
     2221 agcccctgag gagcaggatg tccttcccaa gcggcgtctc gatcccgatc agccggtttt
     2281 cttgtgtgtg tggcataact tcagccctaa ccaggcagga tgttgaaaaa ggccgccagc
     2341 tttgttctca cggcgctcag cggctcaacg tacagaaaaa agtacgcctc gccacctcgc
     2401 ttgttgcggc cgcgctgggc agcctttttg aacatcctgg aatcacttcg agtgccttcc
     2461 tgcatattcg ccctcggtca catctctgat tattcgccta gcagactcga cacagcgctc
     2521 tcaggcctca cgccacctcg tagcggaaat tccctgccgg ttcgactgag atatggacct
     2581 tgttgaccga ttcccctgcc gccatccgcg aaagaaactc tgtcgagagc tccggcaaca
     2641 aggtgcgggt cagaatgtga tccacatttc gcgcgccgct ctccacctcg gtgcagcggt
     2701 cggcgatcgc cgtgaccaag gattcgtcgt aggacatcgc cgcgcgatgg ttctctttaa
     2761 tccggcgcat gatccggctg agctgcaatc cgatgatgcg ccgcatgatg tcgggagaaa
     2821 taggaaagaa cggcacaacg atgagtcgtc ccagaaatgc cggtttgaaa tatttcaaca
     2881 gatccggtct gatggcatcg gccaacgcct ccggctcggg cctggtctcc ggatcggcac
     2941 agagtttcat tacggtatcc gtgccggcat tggaggtcat gatgatcacg gtgttcttga
     3001 aatcgatgtc acgcccctcg ccgtctttca tcatgccctt gtcgaaaacc tgatagaaaa
     3061 tgtcctgcac gccgggatga gctttttcca tctcatccaa gagaatcacg ctgtatggtc
     3121 ttcggcgcac ggcttccgtc aacacccctc cctcgccata cccgacataa ccaggcggcg
     3181 accccatgag cagagagacc ttatgctctt ccttgaactc tgacatattc agcgtggtga
     3241 tattttgatc gctgccgaac aataactcgg cgagcgccag tcctgtttcg gtcttgccca
     3301 cgccgcttgg tccgacaaac atgaagacgc cgatcggccg gcgagggtct tccagcttgg
     3361 ctcgcgccgt acgcaaacgt tgactgagcg catcaagcgc atgggattgt cccaccacgc
     3421 gcttctccaa atgctctttg agactcaaga ccgtgttgat ctcgtctttg accatccgac
     3481 cgatcgggat gccggtccag gcggagatca cctgggcaat ggcctgtccg tctacactag
     3541 gctgcaagag ggggttctct ccctgaaggg cggtcaattc ctttttcaac cttgtcagat
     3601 cggcctggag cgtctcgacc tcttgagcgg tcaacttggc aggcggtttt tccgttgcct
     3661 tgtctgcact cttgtctgta cccttttccg gcgcctttgg cggcgctgcc tgctgctcca
     3721 atttctgacg aatctccgca atctcgccga ccagcttctt ttcctgttcc catcggatct
     3781 tgagttcagc ctgacgcttc tcttccgcgg ccttctcccg ccccaattca gccacacggt
     3841 cttgatggtt gccgcctgtg atgtcttccc gttccagaat gtcgatttcc gtattaatga
     3901 gtgagattcg ccggcgcgcg tcttccaatg cgggaggttc cgcgacctgc ccaagggcca
     3961 ctctggcgca ggccgtatcc aggacactca cggccttatc cggcagctgc cggcctgaaa
     4021 tataccgatg ggagagcctg accgccgcct cgatcgcctc atccaacaac cgcaccttgt
     4081 gatgttcttc cagcgtcccg gctaatcctc gcatcatact gatcgcgacg gattcagtcg
     4141 gctcttccac cttcaccacc tgaaaccgcc tggtcagcgc cggatccttc tcgaaatatt
     4201 tcttgtattc ggcccaggtc gtggccgcaa tcgtcctcaa ctctccacgg gccagcgcgg
     4261 gcttgaggat attggccgca tcgccctgcc ccgccgctcc gccggcgccg atcaacgtgt
     4321 gggcctcgtc gatgaaggtg ataatgggtt gcggcgaccc tttgacttcg ttgatcaccg
     4381 acttgagccg gctttcgaat tcacccttca cccctgctcc tgcctggagc agcgcgaggt
     4441 cgagcacccg caccgtcacg ttctgcaacg gcggcgggac atcgccggcg acgattctca
     4501 gcgccaagcc ttccaccacg gcagtttttc ccactccggc ctctccggtg aggatgggat
     4561 tgttctgtct gcgtctggtc agaatatcga tgacctgcct gatttccgca tcgcgtccca
     4621 agaccggatc gacctgcccc tgtttggccc tcgccgtcag atcgatggtg aactgatcaa
     4681 tggccggggt tcgggaggca tgaggagctc ctcctgccgt cggcgtttcg accccgcccg
     4741 cctggacctc ttccagatct tccgctgatc cggagacgat tttcggcagt tgggcatgca
     4801 ggtcctcagc cgaaatcttt tgcacctcgg gggacatctc gcgcatcatg cgggcaaaat
     4861 cgtcttcggc gagtaaggcc agcatcacat gaccggatcg cacgcgattg actccatatt
     4921 caatggacgc caatgaccag gcctcattga taaggcgcgg gacccgtggc gccagcgtcg
     4981 gggtcctcgc attgcccgtc ttaaaccgat cgagcgtgcg cgtgatatcc cgggccaatc
     5041 gcgattgatc aatgccgtag tggcgacaaa tcttgtggac atccgtattc gacatttcca
     5101 gcaccttcgt caggagatgc tcgatatcca cgtcgtaatg tgtgcgactg agacacaatc
     5161 ctgcggcccc ttccaatgcg cggcgacagg tatcgtcgag cttaccgatg agtcccttga
     5221 gattgatgtt catgtctgtc ctctccgcga gccctgttac gccctaccgg ccctggccat
     5281 catggtggag ccggagaact gtacgtcgtc tgcgtggtga tcaaactctt tggtcttcag
     5341 ccatgtcgtc cagccgagcc tcggggcata ggtgtccgtc gaccggagtc tcgtgggcgg
     5401 cacttcctcg gcccgtaaac gcaattgcac atcgaaatcc aattccggcc cggcaaacaa
     5461 cttcgtcagt tgcacgaggg tcggataggc ccggccggaa ggcaacaacc ggtcgaattg
     5521 ctcaaatttc aacggaccca gctcaacctt aaacgccgcc tgttgatccc aaatcttggt
     5581 tccggccagc gcggaccggc ccaaaatatt gttgtttccc gtcacgccga tggttgtcca
     5641 atctgcctgt tccaagggga gccaggctcc gataaattga ttcacacgta ccggcacctg
     5701 aaagtaatcc gccaggcagc gctccagcgc atccgctgat cgcgggcgtt gtcccagcag
     5761 tccgacataa cggaggaccg atcggtcatc gatggtcaac cgatcacgca gtcccgtcgt
     5821 gcccagaccg gccacggcga acagacagcg ggcgaaccgg tcgtcgctcc ccttcaacag
     5881 ccattgttca aaccccgccg tgcaatggtg tttttcccag gcccgaaaaa agagggagat
     5941 catgcgatgg ttgaagacat cgaaaaaatc acgcagcgtc tggtccttgg cttgcagccg
     6001 ctccagcatg agttcggtgt aatagcgggg aaggacacct tggggaccgg tgagccccat
     6061 aaacgccacg gtcatttcag ccggccgtct ctcgtcgcgg gcctgattga tctgatgaat
     6121 ggcgctcggg gggaaggcaa gggattgatg ggcacggaag cgaacgattt ctttcgaggg
     6181 ggtggcgctc tcgccgaccg gctgccgatc cggatagacg cgggcaagca gccggaccgc
     6241 ttgaaaaaaa tcgaagcgaa agccccgctg cgtgaggtcc tgtgttaaac caggatctgt
     6301 tcccctgctc tgggtggcca ttgtttaagc ggcctttctc gttgttgagt gcgggcgact
     6361 aactgggtaa aggagttcag cgaggcatag agaccgaaaa acttctccaa cacgctcgcg
     6421 aacagataga cgctgctccc cacatatttg ttctcatcaa attccagggt tacttccatt
     6481 ccgcggcaga acccgttcca tgccagcgat gtcggccggc gggtcacccg gcgagaggac
     6541 acagccgtga taccctcgat ttgctgatga accacgctgg aatccgagaa atcatagagc
     6601 cgcagaattt cctgcaacgc ctgcggactg ccctcggtta aggagaggta gttcaacgac
     6661 agatgggaga tcaggcgcca ttgggtttcc cctcccaggg gcggctgcat cggttctgtc
     6721 ggtttgacga gcgcccgtat gcgctccacc ggcgcagcct tttcaagctc gaaatcaccc
     6781 cggatatcgt ccaccgccat ccgtctcgtg agaccacggt tcgtacaggt cgtggagatc
     6841 atgagcgtgt ccccggccgg cacggtagga tccatttcga gatcgaccag cgagagatac
     6901 acctcggagc ctacatcacc tttacggtca gaaggcctgc ggctctggta ccaaaaagcg
     6961 ctctgctcgc ggtccagccg cgtgtgcttg agcgaataga taggctgcac cggcctcacg
     7021 gtctgcttat cgccggacaa aagagacacg gcgtcgatgg aataaatctc gttcgccagg
     7081 ggccgcctca cttcaggaat cacccggtac tcgtgctgtg tctggtcgag acggattggt
     7141 tccgccgtct gagtgaacaa attcaccacc ggtgcacaac cgagccggaa ggtatcggtg
     7201 gtcggcatgt agtcagcccg cggcggctga tcgaggacga tctcgatttc gaccttgctg
     7261 cctatccctg ccttcaccac atgttgcaag ttagcaatct cgatgaacag aaacttctga
     7321 gggaacgtaa aatactcgtg caagagtctg tagcccggca gcgcctgtga cggataggga
     7381 agtaagcctt catccatgcc gaatcccacc ggacgcaggg agcccgaggg cagcaggacc
     7441 ggtttctctc ccggctttcc cccacccgca cgacaacgaa cttcatgcgt atggttcaac
     7501 agcagttcgt acagcgaata ggcaagcggc gcctcgccgt tgaggtagaa gcgcagctgc
     7561 tcgacctgga gatccttaaa ggtgaggccg ccctgggcgt gaagctccag tttaatcacc
     7621 gcccccggct ttccccccgg cagggcccgc tcagccggtt gcactttgac agaggccagc
     7681 atgatcggcc agagggtggt ggaatagcag gtccgaaatc gacattgcgt gccgtcgatc
     7741 ggtcgggaaa ataattgcgt ccccactggc atgacgtacc cgctcgtcag ttttccctgc
     7801 tccggatcaa ggacgaactc gacaatcgac atggaaggga tgggagccag gtaatgaggg
     7861 tacaacatcc cgagcaaggc atcggtaatc tccggaaact catcatcgat cttgagatga
     7921 acccttgcgg caaggaacgc gaaggcctgg atcaaccgct cgacatgcgg gtcctcgcat
     7981 ttattgggtt ccagcaggag gcggcccgct accttgggat aggcctgggc gaattcctgc
     8041 cccaactggc gaagggttga tagttcccgc tcgtaatatt cgagcaacgt ctcttgcact
     8101 atgcttctcc tcgcacggag taggacgagg tccccagttg cagggcggca tcgaagctca
     8161 ccgcttccgg caccggatcc agacgcagca gcgcatcaat gcgaaaatga agcgccgcat
     8221 cgaacttgcg cggcggttcc agcgtgaccc gcactgattt cagccgcggc tcgaacttgg
     8281 tcaaggcctc ttccacgcat cggcggatca gcttgcgctg ttcggggttc aagagactgt
     8341 aactggtgaa atccggcagc ccgaacatca agagagagtt attgacctct gtgtattcca
     8401 cgggcgcgcc ctccaggagt tcacgcctgg tgttcagcaa tgcctcgata tcccgcgaaa
     8461 tcgatttctt gagctcgcgg atattcgctg aggagggggc cggtttccgc atcttgtgca
     8521 gttcgtcggc aaagatctca tccagcaaca tacgattcag cagcgccaca tgaactgtct
     8581 tccggctatc ttccatcagt cgcaccgtat ccggagaaag tttgattcct tcgaatctct
     8641 tacgctcgta cagaagcggc cctctgatga tgcggttgag accttcggtg atggcaagta
     8701 ggaggtcatt ggttccggct ccactccctt cgcggtccac gagttctcgc tcttccggcc
     8761 gaaattgctc cctcaggaat cgcgtcacgg aatcctgggg actcctcagt cgtcccgtca
     8821 gtttcgccgg atccgctatg tctagcgtgt ccagatgcgg atcgcctgcc cgagggtcgt
     8881 catcgagcaa gcggtccaat acagagggca tcactccgct gttttccgag acattcacac
     8941 caatccaccc ctctcacaat cccgtcggcc ccgattcatg accgagcagg tgatgcggaa
     9001 caatcaggag agcgggcaat tcccgcttat ttcttcacac ccttgcgcag atcgtacccg
     9061 cccttcgacg tgccccattc ccacattgcg gcgcgatatc gcaaggcaat cgtctccgtg
     9121 ggcctggcct ctgcgctatg aaatccgacc gacacgttgc tcacgatcac gtcatccaag
     9181 gtgaccttgc agtacacatc cttccctgat accagcgaaa tttccgcctt ggggatttgt
     9241 tcttttaccg cacaagcctt ggcgagcgag ggactcgccg cgtccatttc cttcacgatc
     9301 acgaggtcgg caaaggtcgt gatattgccg gacaaccggt cgccttcttc ccattcgccg
     9361 gcttgattca ccccatagga catcgattga aagacgatct ggtctttgaa cttgtcggtc
     9421 gtcgccttac ctttgatatc acctaacttt aaaaatgcgt tctcctgcct cacctctgcc
     9481 atgatgacct cctccttgtg agtgcggttg gccgtttcac gccttgttct ttttcaatgc
     9541 atctattgtg ttcattcatt cacaaacgca acatatgtgc ccactggaac actcactcat
     9601 cggcgtacaa aaatcgcgtc gacgtgtatg aatgatcggg aatgttggtt ttctctccac
     9661 gcggtcatgt ttggaacttt caggtttcag ttgcgacggt atccaataag atacgccatc
     9721 aatacaatcg gatacgcact gcaactgagg gctgtgagag aaggagcggg attgagggaa
     9781 ccgcttagca ttgaacagat ggattgattg gcgcaagaga agacaatcaa ctcccatact
     9841 gacccttgcc gtttctggct tcgtccgatt cactgacacc gggaccgacg catcgatccc
     9901 ggcttgctcg gcccgaccgg tatctctcat tccccttctt cgaagcggag cctcccgctc
     9961 ttctaccgct tcgccggtgg cggcagctca gccactaacc gcagcgagac cgtgagctca
    10021 tccagttgga agtgcggctt caaaaaagcg acggccttat agaccccggg cttcccagcc
    10081 acttctgcga cttccacccg ggcttccttc aggggaaact tggctttgat ttcctgtccg
    10141 gcgtcttcgg tgctgacgac atacttgctg atccagcgat tcaggaaatc ttcgcaatct
    10201 ttgcgcgtca tgaagcttcc gattttgtcc cgcataatgg acttgagata atgggcgaag
    10261 cgtgacatgg ccagaagata ctgaagctgc gtggagaggc gcgcattggc gtttgccgcg
    10321 tccgtgtcat atttcttggc tttctggcag gattgcgccc cgaaaaacgc cgcaaagtca
    10381 gtccccttac agtgcacgag gggaataaac ccgagatccg acagctcctt ctcgcgacga
    10441 tcggtgatgg cgatttccgt cggacatttg agcgcgattt ctccttcatc ggttcggaac
    10501 gtatgggtcg gaagtccatc cacacgcccc cctccctcga cgccgcggat ggctgcggtc
    10561 cacccgtact ttgcaaacgc atccgtgagc cgggccccca tcgcataggc ggcatttccc
    10621 cagagatact tgctgtgatc cgtgccatcc acgtcttctt taaaattaaa cgcttccacc
    10681 ggcaccgtat ccgggccgta cggcagccgc atgaggacat gaggcaaggt cagccctaca
    10741 tagcgagagt cctctgactc gcggaaggac ttccacttgg cgtaatccgg agtctcgaat
    10801 ttcttggcca gatcccgagg atttggaagg tccgtgaaac cttcaagccc gagcagaccg
    10861 ggcgcagccg ccgaaatgaa cggcgcgtgg gccgcagccg ccacattcga caccttctcc
    10921 agcaaggcca aatcctgagg atgtcgcccg aattcgaagt ctccgatcaa ggccccatag
    10981 ggcgctccgc cgaaggtccc atactcctct tcgtatactt tcttgaacaa cgcgctctga
    11041 tcgaattcga cggccttctc gagatccttc cgcagttcgt ccttgctgat attcagcaca
    11101 cgaatcttca gcatggtgct ggtttcgctt tgcatgacga aatagtgcag cccgcgccag
    11161 gccccttcca gcttttgaaa ctccggcgaa tgcataatgg cattaagctg ggcggataag
    11221 accttgtcga tgtccgctat ccgcgcattg atcgtcgcct cgaggtcttt ggatacccgg
    11281 agggtacctt tcatcacctc ttccacgagg gtggcaatct gccggcgcga ctggtcccgc
    11341 tcccaatcgt cttttccgat gcgggtctca ttgataattt gatcaagaac cccgctctcc
    11401 tctgttcccg ccgccgccgc accctgaggc tgcgcttgtt tcgcttcttc cgccatggtt
    11461 agccctcctt ctgggaaccg ccctcagttc ccagtcctgc ttcttttcca atcttctgct
    11521 gcaactcaga attggcgata atctcctgga gcttttcagc cagcttgtcg ttcccgtcgg
    11581 ttttcgccag aagggcggat agctgtttcc tgacctcgac cagcttcttc aacggctcga
    11641 cctgttcggc gacctgctcg gggctgaagt catcaatgtt attgaagcgc aattccaccc
    11701 cgattttagt gtcgtcattg gtcagcttgt tgtccacctt gtacgcgagg cgagggccga
    11761 tccccttcat cacatcattg aagttatccc gatcggtctc cacgaatttg cgtttgggat
    11821 ccttcaagag cgccgggggc tgctccggct tcccggccaa atctgccatg acgccgacga
    11881 cgaagggcaa ctccttcatc tcaatggcgc cgcccgtctc cacgtcatag gtgatctgca
    11941 cacgcggcgg acgaacccga tccaacttgt gttgagtgct ttcacgcgcc atgagcatcc
    12001 tccatcggta ggttactaag acttctggtc actcggcggc cggctctggg tcagccccag
    12061 cgtttcccgg acattgccga gcacatcctc gcttttgatg acctcctgca gccattcttc
    12121 caacggcatt tctccccatc gcgctgcgcg ctggacgaga taggccacag gactatgcgg
    12181 ttccgtccga aggaaaaatt cagccacggc tcccagtcgc cgcaacgcat ctgtgcggtc
    12241 gaccggcatg atgccggacc cgcttgccgt catttgtacc accggtgcca tgtcggcctc
    12301 ccgaggagca atatctggtt gattcacggt cggagcgccg acaccgcctt tttttcgaac
    12361 aatgctattc atcagagacc ggcattcgct caaggcctcc ttgaggagtc gcaaactcgg
    12421 cgcctccgga cggtattttt catccaggat tcgttcgaac tgttcaaatt cgtcccaact
    12481 ctggttcaga ttctctagga ttgtcgagca gtggctcaaa ggtgtcgccg cgaccgcctt
    12541 gtcgaatttc tccccttcga gcttgccctc ttccaaggct tcggcaagct gccgctttct
    12601 ttccccatct gtcgccgctc cccggcgcag attctcgact tcttgcgatt ccttgtattg
    12661 ccagcagctg taggccggcc cgccgggtgg atgaaccaac ggcatgttga ggatggcgac
    12721 cggaaggatt ttgttcagtg cttcgagccg tccaccgcga aactccaggt cgccgtcctc
    12781 aatcgacgga taaaacgaat cccaatactg ttcatgcaat ccacgcagaa tcttgaggcc
    12841 atcccggagt cccgctgagc catgtttcct gaccaatcct tcggtcagcc aggccgccac
    12901 ctgaaggtcc ttgccctcag tcgacagaat ttttgtcgcg agttgaatag ccttcggcca
    12961 atccgccacc ttcacttcac gcttccagtc gccctggctc gcggcatcct cttcccgacg
    13021 cgcttccttg agcagttcaa aggagacgcc gtctcgcgga tcgaccccac agggcttgtc
    13081 gcccgagatc ggcttgagaa tcgcatcaat atccactgtc ggcacggaag ccatgatgtc
    13141 ctttgcctat tgcactgccg ccaacacctc gaactccagc tcctcgactt ccaggatcga
    13201 aacctcacgg tcatccacga gaaaggtctt ctgccccact cctccgacca atcctgcgcc
    13261 cagatccagc cattcggtca tccggccaag acgcacctta tcatcggact ccagctccga
    13321 tcccgcgtac aacgcgggaa gaaaggcttc acccacgggg ccatcttcta tttcaaccgt
    13381 cgcgggtacc cagagcaaat cccgtagctg tttcggctgt ggaatgacca ctttgtgaat
    13441 gcgttcgaag ggtatccaga tgtaggagtc gcgcacaatc acctccagga tgccccggag
    13501 gcgatcgtcg gaatcacgga aggaggtgaa gggctgccct ttcatccgac ctcggatctc
    13561 cggcctggca cgctgagctc gttcatacag cgctttggcc tcaaccggat gcgcctcgcg
    13621 cagctggtta tgggcgtcta cgtagagtgc gacatgttcg ggaggctcaa acagaaaact
    13681 cggcttgatg ccctccttga aaaatcgccg ccgagtcact tcggcacgga gcatgttttt
    13741 atagacctcc acgccgatac cgactttttc gttttgatgt ccgatgacgt caagctgacg
    13801 ttgcgcccgc tcatagtcac cggtgaagca gaggagttcg aacaggaacg tccgcaacct
    13861 ggcgtcgatc ggatgtcgct tgatgtcctc gtttaactcg gaaatggcag cggagagctg
    13921 atgctgtttg agtaactcat ttgctcccat acaagggacc tctgctgact ggtggaaggg
    13981 attccatcct agtgaaatca caggaaaaga acaaggctat gtctgcggga aatacttgca
    14041 ttccagatct ttccttctac tcgtcgaacc tcttcgaacc gacatcgttt tttaggatcc
    14101 ctcggccctc agtcctgaca cacgatgcag gaacgcctcc aaggtcacgt ctccatcgtc
    14161 gagaagcgca cgtccagcct ctgccaaatg tccggcatcg tcgttctcta acagctcgtt
    14221 tggccggcct tccggacgga tgagagaaag cagaagttga gggagcggtc gttccaggga
    14281 gaccaacagc aacggcttca ccttggaagg ccatcgattc cagaagagga tccctgcttc
    14341 cggtctgtgg cccgaggcgt gagatgcgag atccatccaa aatgggaggt cgtaggtctc
    14401 ctccttgctt tctatgagca aggggaattg ggcctgccag tggcctcggc tcgcggaatc
    14461 cgatcctgat cgtgaatgga gcaaagaccg aagcagggcg tatttttggg tacagtcgaa
    14521 cccgccaaat acatcggccc agtgatcacg catccggcgg tgcagcagct ccacatggta
    14581 tcgcgtctct atagaagtgg actcaggcac ctcaaccggc gacagcgcct gtagccggaa
    14641 ttgcaggcgg ttgagatcgg tttcggtgct gacatcctcc agcaaccgat gactctgccc
    14701 aaaaaactcc ctgaagtgca tcggtgcgca ccaggggagc tcatgaaact ccttcctctc
    14761 caagagcagg taaatcaaaa aaggaaactc tcgccccgcc ttgtcctgac tcggtttcag
    14821 gaggcccgcc aggaatcgac tccgatcggg aggcaagtat aaaaacgccc actgatcccc
    14881 ttgcaggaaa tccatggacc aggcaggccc cagggtggat tttgcatgga acatcccctc
    14941 ccggatccat tgatccaatt catcgatctc cgctcctgat gcctggcatc ggataaattc
    15001 cgcagacaaa ggaagttttc cgtaatagcc gagggtagag ctatacattg agcttatgtc
    15061 acatgatcta cacaggtgaa ttgctcgaag aatccgggac ggaacggatt cttcgcgctc
    15121 ttggccctca cgtcatatcg aatttgtaac ggcttgaaat ccggggtttg cagttcccat
    15181 tggaccttca ccttgacatc gctatgatcg gtcgcctcaa tcacaccagc cctgataagt
    15241 ttgaacagtc cccagacatc tttgaagtcc ctcgtgaccg agttgtcgcc aaccttgaca
    15301 accagggcgg ctccaagaga aggtgtcggg ccaggccatt tcatctccca ccagtcttgc
    15361 ggctccatac gataccggag cggctcgcca ccgatgtcca gtcgtatctc actaacgctc
    15421 cgacctccag ccccccctgg cggataggga tagacttcga tcaccgaccc caaatccgga
    15481 gatgctttcg taaagaggct ctctgaaaca aacctggcct tctccaacga ctcgatgaag
    15541 gcatcagaga cagtgagcgc gaccccgctc catgctctgc gcactaagtg gtcacccctc
    15601 tcttccagaa atggacttaa gtctgcctgg tagaaacgcc agagcgcccc cttatccgga
    15661 tgcaaaaaat tgacgacatc ctccaccaca gcatcctcgc cggatgaccg gaagggatat
    15721 ttacgttcaa tcgcgcgctg acaggcttga aacacatcgg tgccccagcg ttggtcgata
    15781 tccaaccgcg tccgctccaa caccccacgc atggtcatca accatggctc cgacagcagc
    15841 gcccccatgg attctcgcaa ttcggcatcc aacttcttta gcaagaaacc tgtcttctcc
    15901 atccccagct tgagctcgtt cgatcctcca gccacgatgg tcctcgcaag gattttcgtg
    15961 ttcgggttga tggtctcctc aagtagaatg ggccgaaacg cctcgtgccc cttccgcaat
    16021 tccgtcaggt actggatgat aggcgcctct tcctttgcat ccttccgggc agcgatcaga
    16081 tcatgcaatg caagaaaatg aacggtgacc ccgccgggaa actcagccgc gtccggcgca
    16141 cgtggtggtt tctcgagctt cttgccgact gtttccagcc ctaacttttc cttcaccttt
    16201 cccagcaagc cgactgccga ttcctgaagt ttcgcaatgc cttccggctc cggcacggta
    16261 ttgcgatcga ccgcttccaa tattcgcagg aagggcgaat ccgtctggct caaggttgag
    16321 agcaatttct ccatatccgt cggagtaacg gcgggaccaa gtgagaggga tttcaagaag
    16381 gctcgccaat gaaggcggta gtcttgaaag tagagtgtct tgatcccccg ctccagatcg
    16441 atctgttttg tatcaggttc gcccacgacc caggcttcgc ttccaagacc ttcgagaacg
    16501 cggcccatgg caatctggaa cggtcctttc caaccctcat aggtgaaggc accgggaatc
    16561 agatggtcgc tgacgaccgt cccatggtga cttccttgaa gggcagtctc gacagaaaat
    16621 ggtgcgggcc gaagggattc gtcgatttca cgaaggctga ggccgtagag ccggtccaca
    16681 agggggatat cgcgcaagcg ctcttgcgcg cctgccacca ggtgtttgtt taactcaacc
    16741 cggccctgtt gtgcgcgagc cagcacaccg gtgtagagag ccatatgtcg ttggatactg
    16801 gcttgcacca agggcggaac cgcataggtc gcgtacagac ccttcagccg atcgttccag
    16861 tgggcggtca gccatcgctc caaatagggg ctgctcaggc gcttgggttc accgagcatg
    16921 atgtaggctt tgagcatgga ataatagtaa tcacttgcgc ctatcgcctc cccgcgcgac
    16981 tctcccgacg caaattgatc gagttccacc tccatggcac gcttggtcgg aatgagaaga
    17041 atcttctcaa actgccgcgc gtagacttcc tcaagatcgc cgagcagccg ctctcctcga
    17101 tacaaacccc agagacggaa aggcacaccg ttctcgctat aggagaccag ttcgcccaaa
    17161 cgatcaccca ccctatcgag atactccaca tttttttcga ggctcgccgc gtccgtcact
    17221 gcgatgtctg gcgcattcaa cgccaccgac agggtattgc tgaccagcgc tctattcccg
    17281 atataggaga aagcaagcgc gatcactgag attgcgacaa acagcgttcc cgccgcaaag
    17341 gcggccacgc gcaggaaacc ccgttgttta tgaatggcgg tcgagggccc cgcgaggaat
    17401 tgatcgggaa agatgacatc ggtgaacaga tccttcagaa aatagctctt ggtctccgtg
    17461 ggcgaaaaca ttgaggagac catatctccc agcccagaag cgcgactgat tgcccctagg
    17521 atgcgatcga tcggcgcccc ctcttgcgtc ccactggtca aatagaaccc gcggaacatc
    17581 ggattgtcct ggtacgggtt cgattgaaac aacacttcga cgaaatgggc caacttgtcg
    17641 gtcgccgaag cgagttggag aggaaaggtg aaaatattca acttataggg tccgcgcgca
    17701 ttcaccaggc gggcgagccg gcgggatttt acggcggtga gcaactgatc gaactcggtc
    17761 ttgaaacgca cctgcgccgg ctctttcgca gcgctctttg gaaacgtgca tccccagatt
    17821 cgttcccgtt cggccttggt gagttcatca aagaattcaa caaagcccct caccaagtcg
    17881 catttcgtga agatgagata gatgggaaag gtaattccca gatgcgtcat cagctcatcg
    17941 atgcggctgc gaattttctt ggcatgagcc tccagctctt cttcactggc ctgcatcaga
    18001 tcggcgatgc tgatcgccac gaggatgccg ttgaggggtt tttctttccg gcacttcttg
    18061 agcagatcca aaaagcccag ccattctttt tgatcttcct catgggtgac atagcgcccg
    18121 gccgtgtcga gcagcacagc atcattcgtg aaccaccagt cgcagtttct ggttcctccc
    18181 attccctgca gcccctgccc cgattcaccg agatagggaa actgcaaccc ggaatgtcga
    18241 agggcggtgc tttttccaga cgcgggcggc ccgatgaaca tgtaccaggg caacgcatag
    18301 agagctgctt tccccttgac ccccttcccg atcgtagact ctttcagagc cgccaggccc
    18361 ttatcgaact ggcggcgcaa cgcttccagt tcttccttat ggtcgggcct ggccgccgcc
    18421 acttgcacct gggcctgctg ttgcagcgat gcctccaatt ggacggcacg gcgttgggag
    18481 aggatatgtt ggaggacaga gaaaatgaac cgtaataaga cgatggcgat gatgacctgc
    18541 acccggctct cgaccgattc cagaccgatc gtcggaccgg ccaaccagac caagcagatc
    18601 aaacagacca gtccgaactc gaccaccaga cggcgatgcc ccagcatggc gaccaacagc
    18661 gattttatcc tcgcgaacaa gcccattatc cccgcacctc ctggaggagc cgtttcaatt
    18721 catggccgac atagccggca tcctgtccga tcaagagaga cagtccgaga tagaacagga
    18781 ggaccaggga ggtgctggtc gcaatgatga cccagacggg aagctcgctc ttgaccatct
    18841 ccagcaactc ttccggacgt cgcccgcgcg gcgaaagcgg tgggatctcc ccccgcttac
    18901 cttgaatctg gcgcgaaagg tcttcgacca gccctcgtaa tttttcacga tcctgcagct
    18961 tgtattggcc ctcgaacccg agcatgagac agtaggtgta gagctccagc aagtcggcgt
    19021 tcatcggaat gccgccgcga atattctcca gtcgtttaaa gaacccttcc cctgccgtaa
    19081 attcgccgaa taattcgtat tgcagcggcc gagcggccca ttcgtccttc tgcggccacc
    19141 gcgagttgat gagcatctcg tcaagaaatg cggccacagc gtaacgagct tgggtcaacg
    19201 cctcctgagg gaagccggag gtgcggcctc tttcctcagc cgattgaaac aactgctgga
    19261 ggcgggtacg gaggctctcg acccctcccg gattggtggc atcgcggagt tggacgccga
    19321 gcaccaacag atcgttgaac acttcactca gggagttcgt cttttcggcc tggacggtga
    19381 ccatagctga ttactccttc gttgcgagaa gctgcaaccg aagagattgg aggctttgag
    19441 gaacatggag cgcaacaatt tgcgttttct tgatttcttc ccagaacact cctcgatcat
    19501 ccagacgaaa gtattgctgg cccacccgca ccggcaacgt cgcaggaggg cgcggggtat
    19561 ggtacagcct gacgccgggc atcgcggctg ccacaatttc cttcagcttg gcgggcgagc
    19621 cgatcctgat gaattgcggc acttgctggc ggatctggtc ctcgctgaga tcgccggcga
    19681 ccactaagaa aaactgagag gacgcaaaaa ggcggctctc cggaacccgc gccacccaga
    19741 cattctcccc gctggcctcc agcgcgatgg tcaggtaatg cgtgggctgg aaccgttcca
    19801 cgatggagcg gatggtatgg tccaggtcct cgaaggtggc agcgaggttg tcgtgtcgat
    19861 agggcgggag cgccaggact tccaggggtc cggccagcat cgagagcgct ccggtcaaac
    19921 gggcaaggat gagatacaga aactccggat gcactttttc aacaaggttc acatgttgca
    19981 tggccggcaa atgggccatg agcgcggaga ggagatgata cttccccaga tccattccga
    20041 tggcttccag aatgccacgc tgagcattac cgtaggatcc gatttgagcc gtgaggagtt
    20101 cgatgaggcc gcggagcagc cgcatgaggt acggcgaggc ggaaatccac agacaagccg
    20161 ggatgtaggt ttccttcaat gcgacggctc ccgactgggt cctgagcaac tcgccgatct
    20221 tcaagaccac catgtcgctc gtgggttcgc ccgaaaataa gatgcggagg ttctttcgtg
    20281 cgaccaggat ctcctgagga ttaccgcccg tcgtggaatc cttccgttcg acgtaggctg
    20341 aggaataccg agcggctcga gagccagatt cgtcgcccaa cacgcagttc acgccgtcca
    20401 tctgctccac cggcaaggcc agaaacacat cgaggtgatc gaccgaggcc gggaacaggt
    20461 caccgatgag ccgcgtttgc ggagccacgt cctcttccgg aatcctgacg accacaccat
    20521 ccggcaacag cccatgaaaa ccaagcaacg tgactcggcc gttggacaga ccatcatgat
    20581 cgaagtccag cctcaagacc ccatacccga acgcaaccaa cgcacgaagc cgttcattga
    20641 gatgacgctc gtaatactgg tcccactgtt gaaataagtg cggagtgagg agcatcccct
    20701 cgctccagac gactctttgg ttgttctcca tggtgatgac cgacgccttc cttattcgtc
    20761 cttcaatttg agcttgtgtt catccagaat caatttgacg gtcggagttg taaagggaat
    20821 ccaggtggac tggtccgacg acacgacccg ccgccagagc tctccttccg gcttacgaaa
    20881 caacgccatg actcccagaa attgaacccc cttcttctga tccacctgca attctatgga
    20941 ggtcttcgtt tcaggataga ccgtgacctc cctccgttcg agcacatctc ccgccaactc
    21001 tttctcgtca ttcctccaga gtgcccggaa atccgctcgg tcaaaccgct ccctgttcct
    21061 caactgatac acgcgcacga caacggacaa gggggcaccc gattcatcgg gattcaaccg
    21121 ataggtcggc aggagtgtga cgcggacatc tttgatgccg ctcccgcacc cggcggtgat
    21181 tcctcccacc atgaggatga tcagaaatac acacaggtta ccgaccacgt tgagtctttt
    21241 ggacatccaa gtagcctttc gcgaaatgtg gcgccagtat ttgttcaaag atcctgactt
    21301 cctcctctgt aaaccgccgg tgctttcgtt tgaagtgctt ccaggcgccc cattgtttca
    21361 ggagacggaa ggaaggaggg agacgcaacg gcccgatttc taccggactg acctcaaatt
    21421 ctgcttcgat tgtcgccgga tcgaactctc tcagtacggc gcgaagactc tcttcaaatc
    21481 ctttgatcac cccgatatga tggagcgcca aatcgcggaa gacccgttgc aggtccgcca
    21541 tgacggcttc tgcgtccttc ggattagcca gcgcatcgaa caggtaggcc ccgacctgtt
    21601 gctcgttttc cgcctgcttg atctgattag gcgcccaatt gagaatacgg gtcgcctcca
    21661 cctctaattc acgggcgaac tgccgcctgc ctcgtaagga ctccaccaga ttgccaaacg
    21721 taatctggag aacctgatca atccgccgcg cgaactgacc gatgaattca gcgctcgtcg
    21781 gcgagggaag tcttaagcag tacttctgcg caatagcgag aaggccccga taggcggcct
    21841 cgcctgcagc ctgatccgta cgcactggtt gggcaggagc cgcttcggca ggaagaggag
    21901 cggccgccgg ctggccgagg cgcaagcaca agccggcgcg gatttttccc atcagggatt
    21961 cgcagaccga cccaacctgt tgtgccaagg cccggcgcag catttccaat aattccgctt
    22021 cccgctcctg ggttgtacat ttctgtaatt cggcgtaccg acgacacagt tcgtacacca
    22081 gtgcgtctgc gtcgacgccc tgaacggcag tctgtggctc ctcaaccaca ttatgcgcca
    22141 tgccattggt ctgcggggtc tgtactgaaa acagaatggt gaaatcaccg atgacgattc
    22201 ggtcgccctc ctgcaaggga tactcccgct gaggcaccag cgactgctca ttcatcctgg
    22261 tgccgttggt gctccccata tcaaccagca cgcaggcatt ggctttacga cggatctccg
    22321 catgccgaga agagacgacg cgctgaggat cctgtaatgt cacatcgttc gaggaagccc
    22381 gtccgatcgt cacctctccc tggtcaaaga tatgttccca tgacttgccg tcgccaagat
    22441 ccttgcgcgg atgaatttcc aatcgaatag ccatcgtcca cactacatcc gctcagcaag
    22501 cgcacgatca cagacccgca tgagatcacg atgacgggca gcggcgtcga acaactcgac
    22561 tctcgcctgt tccgccacac attccgctgc acacatggcg ccctgcaacg cttcgggaag
    22621 cggtgtatgg tccgttgatc cctcactgcc acagaagctg aagccgcgaa tccctggtac
    22681 caaccgcaac ccacagatca gtgcctcgta ccacagcgat tccggaatgc tgatcgaggg
    22741 tggcaggaag acttcactcc gttctttgag ccgcccgctg acacgcgtaa agagcgtcca
    22801 gtgatcatcc ggattcaagc cctggacgag aggctcgagc ctctcaagca ctcccgcaat
    22861 gtgttgttgc acctgctcca atgactgagt ctgatggacc agtagcccgc gaaagagcaa
    22921 catcccaaga ctgtacagat cggaggcatg gtgaaacgcg cgatagatcg atgcccctgt
    22981 atcggaaaat acttgctgtt tcgcccgttc caattgggtc caggttgcgt tgggaatcgg
    23041 atccgtcata ccactgacga cgagcccgcg ctcagccttc tccacccttc tcgcccacag
    23101 aggaaccgga gccgtcccgc ttcctggcag cctgatggca aggtggaatg tgtcctgttc
    23161 ggagcagtcc acgaatgaca cggtctctga aaatacatgg gcgcgtaaga gtccgcgtga
    23221 gagctgttcg tctgtgtcat cgggaatccg ctcaattgaa cgaagcagga ccgtgaccgg
    23281 aagttcacgg cccatgggcc attgcctcat cacaggcgca gcatagtcgg catcgaggtc
    23341 tccagaggga acgaagatat ccctggccat ttccttcggc atggtttcgt ggaccaaaga
    23401 ttgcgccccc ttcgcaacgt ccaattccac cgcaaaaagc caccgtgccg ggagccatgt
    23461 gaacgaatga tcactgacac gcccactgat gtgtgcggga tccagagaca aatagggacg
    23521 ctggtagagt cgatagaatt cagccacgcg acgacagagg gaggcgaacc cgctgagctt
    23581 cagccataac tgttccagag ggagccgtcc tggctcatcc tgaaatagaa actgccgacc
    23641 ggaggagaat gtggctcgaa atagcgggag tgagtgaaca ggctcaggga tcgttgacca
    23701 atcggctcct cctagcaggt cacagacctt gtcccaatgt aagcaaggag ccgcgagttc
    23761 agaatcggga gcctgtgaga gaggactcgg catagggtag tcacgtcacg tgagcggaca
    23821 gaaccccact gtactggtgc agaggactcg gtgcatcttc gaaggacgaa actattcctt
    23881 tcgtcgaagt aaatcaagca catgcaccgg gaatggaagt atttcccctc atgttccatc
    23941 agggggcttc tcacgcccag gatgccaatc attatttgtt cgtcaggtcc ttggccagcc
    24001 tactgcctga atatcaatca tcggagactc gttgagaaag cgccgcggtt gcggtagctt
    24061 atgcacacca atcctgatag caggcgagtt ccccatcccg tcactaatag gatgccggtg
    24121 ctgaaaagtt gccctcttca ccttgggtga acgccatgct ccctgctctt gagagctcaa
    24181 ctccgcgatg gcacggtgca ggactgagtg atgtcggcct gctccgctcg tccaaccagg
    24241 acgcattcgt cgtggacaat aacctagggt tatgggtggt cgccgacggc atgggagggc
    24301 gagcaggcgg caatatcgcc agcagcctga ccgtcaaggc tcttcttgac tacttccacc
    24361 attccaaaga cagcggcgag tccgctatct tcaaacgggg cgatggtgag gcatcagttc
    24421 aattgcgcca ggccattcga gcggccaacg aggctgttcg gcaaagggcc cttgaagaac
    24481 ctgcattaga cggcatgggc accactatcg ttgcggtgct ggtgtcatcc ttgtctccac
    24541 tacaactcgt gatcggccat gtcggggaca gccgggctta tctgtatcgt ggccaggaac
    24601 ttcgactctt gactcgtgat cactcgctgg tcgaggatct gctcacacga ggtcaaatct
    24661 cgccggagga agtcggcacg catcctcaac gccatattct ggtccgtgcc ttgggcatag
    24721 aggggcagac agaccctgat atttccaacc aggcgttcca atcaggggat atcctgctgc
    24781 tgtgtaccga cggcattacc accatgttga gcgaccggca catcatcgcg cagctcaccc
    24841 gctcggctga ttcacctgat gaggcctgca gggggctcat ctctgaagcc aatgcgcgag
    24901 gagggaagga caattcgaca gcggtggtcg tgcgcttctc ctgaaacagc gtaaccactg
    24961 ccatgctcct gacccgacga gacgttctta attggagatg ccaggactcc cctctaccct
    25021 atgtcacttg atttcgaatc cctcagctta acggaaatca ttcggcttca aaatcaactg
    25081 tcggcgttgc ttgcccggcg gtttgaaaag actcacgcac ctgccttcag cgacgtgaca
    25141 ggctcgacgg cgtattttgc tcgttttgga aatgaggcag gtcgcaggct tcaacagcgc
    25201 catgtcgatc tcatagccaa gagcctgcag ggatcaggcg gccgtattgt ggatacagct
    25261 ggagacggag tgtttctctg cttcccgaac gtcgaggcgg cggttgaagt attggaacgc
    25321 ttccagaccc tgcgcttgca ggagaatgcc catctccctc ctgagcatca gatgacaacg
    25381 agaatcggca ttcattgggg cacagtcttg acggatggag tcatcgtcac gggcgatccg
    25441 gtaaatctct gcgcgaagct cgccgctacc gcgcaaccag gcgagatccg catcacgaaa
    25501 ccggcctttc tcgaactgac tggccaacgg cgacttcgct gccggtctat tggaccagtg
    25561 aaactcgccg gggtacagga gccgatggac atgttcgttt ttgcctgggc tgatcctgtc
    25621 cgttttccct ctgctgtgct cattgcggaa acaggagaac agatccccct gccgccgcag
    25681 ccggtcatta ccttcggccg gttacgcgaa atgaacggca cccacgcgaa tgacattgtg
    25741 ctcacccaca aagatccgac tcggacgcag catatcagtc gatggcattt cgaagtacgg
    25801 cgcgcaccgg aaggactgat tcttcgctca gtatcgactc aaccaacaga ggtgaatgga
    25861 agggtccttt gcaaaggaga ggaaacgagc atctcggccg ggaccatcgt tcggttagca
    25921 gatgtcctga ccctgcgatt cattgcaggc gcatcagacc aggcatctga agacaacgcc
    25981 gtgaccaccg tttcttagat tcccacccag ttctgtgaag attgtgcatt cgccggtgct
    26041 gcacaggagg agcgctactt ccggcgtgga ggtcccggct tttccttggt ataggtatcg
    26101 actgctttgc tggtcgtgac caagggtacg gaacgatcca cgccggttaa cttcgcccct
    26161 cgactgccct tcttgatgcc ggactccagg cgttggaggt agtctcggct ttcgtcgcac
    26221 cctcgtccgg catcataacc gctctcctca atcggcacag cgctcaacat cgccttaatg
    26281 gtttcagcgc caaacgggga tgtgatgagg atatgttccg gatctgcatc gcccccgaac
    26341 gaataggtct gtccaccatg aatcagcgcc tggccgcgat acacattcgg caccaagtgc
    26401 accacgcagc catcagccat gaaatagtcg agtttgagat aggcatcgcg ctcagactgc
    26461 acatatacga ccaagcgatc tccctcgatg taatgaccat cggccttatc gacccatacc
    26521 ctcaacccga acccttgcga ttgcggcagc agaggagttt gcgccgcttg tgccacgtgt
    26581 ttggcgttcg ctcgttgctt gatcaaatct tcgaatgaca ccggactcag tttcgcagtc
    26641 accgtcagac aaatttcctg ccccttcttt tcctcatttt ccaccctgat ctgttccagc
    26701 atcccggcgc ttaccgtttg aatcagatcg tcttccagct gaaacttcct cacggtggag
    26761 gtggattcca cgaacacacg atgactgcga acggcttgct cctgtgcaat gaccattgcg
    26821 gctcttttgg cttgagcagg cgtttcgttg tcgccatagg agtaacagcc cgtgccacga
    26881 acagtctcca attcatggct cccggccttt gtctctgcga tgacgtgctg agggttgaag
    26941 agcgacaata ggacgcagag cgtaagcagt gcatagaacc atggacgcag cttattcata
    27001 tgacaccctt accccatcaa tcagcaaaga ggactgttgt gctcacgcca ccaagagagt
    27061 cgggaaaata tagacaatac gaccctcatt gtcgaggact gccgcgtcag gaatacacgt
    27121 attgtggcca gggtttcttc ctatcctccg gcaatctcca ttgcagcaat cctggcttcc
    27181 cgatacaatt gtgccgggca tccaagtggc gttcttccta tgatgtcatg ccatagcgag
    27241 cagggacgca tgatgccgtc gatcagccgg gaaagaagga ggttgcgtca tggccaaggt
    27301 gcttgagcct ccaacaaccg ttcagaacag actcctgccg ccgccctttc acagccaagc
    27361 atcgtacgtg atacccgaaa tgaaaactat gacgcttctt gcagttctct ttgtctgcat
    27421 cgcagttgcc ggccaggcct ggacaaccga cacgctcgca caagaaccgg tcactcctga
    27481 gaagctgatg gaacaagggc aatccgccta tcaacaaggg gccttcaccc aggccatgca
    27541 gcattggacc gaggccggcc ggcgttatga acgggatggc aaaacacggg agcaaatcaa
    27601 ggcgcaagtg aatctgacgc aagcgctcta tcaaaccgga cattataaag aagcgggagc
    27661 gatcttagtc gattccggca aactcgcgga taaaattgga gaccggctcc tcaaagccat
    27721 tgtgcaggga cggcttggtt ctatcctatt tgccctgggc gacaatgagc cagcgttgcg
    27781 cgcaattgaa gaggggctcg cgtcagccag ggcgctcgac aattcggctc ttgccgccac
    27841 catgctgaac gatcagggca acattctcac ggccgacggg cgatattcct ctgccgtggc
    27901 ggcctacacc gaatcatcca tcctggcaaa agcctcccat caaccggcgt tgacgaccat
    27961 cgccctgatc aatgccggcc gagccacgat tcaagagggg tcgctcgaca cagccaaggc
    28021 gcgcttggac atcgccgcgg aggaaatctc ctccatgccg gactccttcg acaaggccaa
    28081 tgcctggctc agccttggag acgcctatga agaaatcgtc caacctcgca tcggcaacgt
    28141 ctcttcgaga aatgtggtgc aacggacatt gctcgcggcc aagagttcac ggggtgtcga
    28201 actacagccg ggaacgcctt cgacgaagga gccggctgca accacacctc catctgatac
    28261 acagccctct caaaaaaagc ctagccctct gcccgcccca cccatccttc gccaggcggc
    28321 ggaggcctat tggaatgcga tccaagtcgc gaccaagatc ggggattcac ggaatgaatc
    28381 ctacggctgg ggctacttgg ggcatctgta cgagatgcag catcgcacgg atgaagcgct
    28441 ggatctgacc agacgcgcgc tgctctcggc tcaacaggtc agctccccgg agtcccaata
    28501 tcgatggcat tggcagaccg ctcgtctcct gcggaccaaa ggccagatca acgacgcgat
    28561 tgctgcctac cagagagcca tcgagacgtt gcaacccatc cggtcggagt tcatggtggg
    28621 cggccaaaac cgccgctttt ccttccgcga gacaaccggc aatctgttct ttgagctgtc
    28681 ggatctgctc ctgcaacgag cctcgtccac acctgaccta gccgcccgcc agcatctgct
    28741 cgggcaggcg caggacacgg ttgagctcta taaggcggcg gagctgcaag actatttcag
    28801 agacgagtgt gtcgcgacgg cccggtcgac gagtacggcg gtcgcccaag aatccaaatc
    28861 aacggctatc gtgtatccga ttatcctggc agaccgcatg gagctgctcg tgagcatgcc
    28921 ggccggactg aaacaattca tcgtgccggt gaccggcgag cagctgacgg aggaagtacg
    28981 agcattccga ctcggcctcg aagaccgttc caacaacgcc tatcgtcccc atgctcaaaa
    29041 gctctatcag tggttgattc gccccatgga agcagacctg acgactgcgc acatcaccac
    29101 actggtcttt gtgccggacg gacccctccg gacgattccg atggggccct tgcatgacgg
    29161 cacgaagttc ttgattgaac gttatgcggt ggcgaccaca ccggggttga cactcacgga
    29221 tccacggccg ctcaatcgga aacagatccg cttgttctcc atgggactga ccgaagcagt
    29281 gcagggattc ccggccttgc cctatgtcgg caatgaatta aaggcggttc aggctattta
    29341 cggcgggaaa cagttactga atgaggagtt tcgcgccggg aaggtcgaac gtgatctcaa
    29401 tgaacagcca tacaacatga tccatattgc ctcgcatggc aaggtcgaaa gcgacgtcac
    29461 gaagagtttt gtgctcacgt tcgacgaccg catcacgatg gatcgcctga gccacctagt
    29521 gggattgttc gaaatgcgca cggtgcctct cgagttgttg acgctgagcg cctgcgagac
    29581 cgcggcaggg gacgatcgag cggccttagg attggcaggg atggcgatca aagccggcgc
    29641 gaaaagcgcc ctggcgaccc tctggtttat cgatgacgaa gccacggccg agctcatcgc
    29701 cgagttctac aagaatctcc aggatccagc cacatccaag gcgatggcac tgcaacaggc
    29761 gcagctcacg ctgttgaaga atcccgaccg agctcatccc agcttgtggg cacctttctt
    29821 gttgattaac aattggttgt aaatcgactg caccaccgta ccttctgcag gcactttctg
    29881 tctcagtctg atgctcctcc acgagcataa cggagccctt ccctctccgc tccccatcct
    29941 agccagctga aaaccgcctg ctatagttga tggagagcga tgctgcgtac gtggcacttg
    30001 atctcccccg ccgacggagc actgcgcttc atggattctg atgacaagcc cctttcccat
    30061 tgccatgcct gcgttccgca tcactatctt gattggtctg ttcgttcttt cgaattcgat
    30121 cagtgaggcc cgtttttctc tcggagccga aaccctcacg ggattgtcgt cgacaaccac
    30181 ctcctccaca tggccttccc tcacatatca ttcgccaaga gaacggcctc acgccaatgt
    30241 caaaggggaa gaattcggat acttcccacg ggtcgaggcc gagccgctcg tggcgacggt
    30301 ggaggatagt cccataaccg gcgcgagagc aggccccgtc acgcttgccc gcacgcagcc
    30361 cccccttcat tcaggaacca ggaccaccgt gctcggcgac ctctacctcg ccgacgacca
    30421 tcccatcgtt gcggcagaag cggcagagct cctggcttcg accgccctgt tccttgatcg
    30481 agatccgata gcgcatctca cgctggaggc ctattgcgat gagcgcggga cggaagcgta
    30541 cagctttctg ataggccggg aatgggtctt ggctgtcgag cgtcgattgc aggagatgcg
    30601 agtagaggac aggcgggtga cgacggtcag ttacggtcta caacaaccag cttgccagga
    30661 agcatcgacg acctgtcggg aagacaatct gcgtatgaaa tggtcgattc gcctgctctc
    30721 tcccgtcaca tcgcagcagg gctgtctgat ccggctcaaa ctcgcgacca atactctgct
    30781 cgatcagaac gctttgctcg tagccaatca accgtacctt cgtcgcatcc aacagggaga
    30841 accctattcc gccaaggctc cctgacctcc ccgcttgtcc ggtaaaaagc ccaacccaac
    30901 agccctaatc tcgtcgtaga gaccgcctca cccaaaagcg caagcgagtc cagcacagac
    30961 tgctagcccg gattattcac gaatgccgtc gcgaggcgtc cgagaccgaa gcccgccggg
    31021 gcttctcgca agcgaggccg acgtaggcgt acttgcagtc cgtcgaggag gccgagcgag
    31081 aaaagctccg ccgggcgaga ggatcggacg acggagcagg tattcatgaa tagtccgggc
    31141 tagaggacgt tgagcacgaa ctgtatatgc accccctcat cctgcagacc gttatggggg
    31201 ttcgagacgt tattcgtcac gagtcgcctt ccccaataca cattgaggtt tgccaccgtg
    31261 gtgatattga accgaagtcc ggcccccacg ctcgccaacg tttgcggatc accgctcact
    31321 cccttggccg accaggaatg gccgacatcc gcgaacggac agaactgcac atagggaaac
    31381 ccttcgttgg aactccacac aggaaagcgg gtctcgaact gatacacgat ggcgttatcc
    31441 cgaagcaggg tattttcgcg atagccacgc acactatacc ggccgcccac ggccatttgc
    31501 tccagaggga acagacgatc attcgccaac tgcatattca gaaagttcaa tatttcgata
    31561 cgcgattcaa accgcttcaa ccattccgcc tgcccgagcc aggaaaaaaa ctgaccgtcc
    31621 gccgctcccg gcgctccgtt cacggtcgcc cccagcacat cgactcctgc cgaaaaccga
    31681 gagcggacgg agaacaccga ctccgtcgtc cgatgggtcc attcctgaat gaagcggagc
    31741 gcggcaacat tgccgaagcc gttcttcatg ccgttcacaa aatcgaacgg cgtgccaagg
    31801 aggctgttgg cattctgctc gtactcaccg acaagcgcga tcgtgacctc atcgttcagc
    31861 gtgcgataga cgggctggcg caacgacagc gtgaaaatct gggtctcgct cttaatatcc
    31921 agaggccgga acggatcttc caccactttg aacttaaaaa accggtaacc ggcaaagaag
    31981 gtggtgtcat atcggttgag gggaatcgca tagcccgcat caatcgccgg aaacactccg
    32041 atcttaaccg gtgaagcctg accggaagcc ccgaaactca tactcaacac atcgccacgg
    32101 cccgtgaggt tgcgatgggc gaccgtcccg cgcagctgat tctcgccgac cgtggggttg
    32161 atgtaattgt tgtactcgac gaaggccctg acgggattcg cctcctgcac cgctacatcc
    32221 aaaacggctt caccagggac tgcgcccggt ttgagttccg cattcagccg ctccaaccgg
    32281 tcgtcctgca agaggatctg caatctgtcg cggagcgggt tcatattgaa cggaggtccg
    32341 gcactgagct caatgcggtc gcggaggtac gacgggcgaa accatttcgc accttgaatt
    32401 cgaacctcgc taagcctccc ttcaaccacc tgcatcgtga tcatcccatc gaccaccgct
    32461 tgatcgggta gcacggcccc ggagttcgga tagcccttgt cgatataggc cagcgtgatc
    32521 tgccgtcgca gttcctccag gtcttcagtc gtcacctccc gattttcgta aggagcggtc
    32581 agttgggcca actcctccgg cgacaacacc gtactgcccg tcacctggat ctgtctgacg
    32641 aacacccgca acaccgggcc cttctcaccg agttctctcg gcgaaggttg gacaggggga
    32701 agaatatctg cgggaggttc ctgaggccgc aggggtttct tctgttccac cggcggcggg
    32761 aggccggatc gcccggaagg atcgatcacg ggaggcaggc cgacttgcgg ataacctgag
    32821 tcaggcagcg acaggccaca agcgactacc aggagcaagg agaagggcac ccctcgccag
    32881 gaactgctca gccaggttgg tgcgaagttc gaaccggatg gcgcgatgcg ccgggactcc
    32941 gcgacgtggc ggccaggccc attttcttct ccgtgatcta tccggcacct catgtcggca
    33001 ccttatgatc gacagccggc aaaaaccgtc aggctgctac gagacgaccg gtcgaacccg
    33061 ggaagtccga gacggatggc tgacaggctg gaggctgcct gcgacctgat gttggacgct
    33121 gcctcatcag gctccagcag gagcgggctc ggaatgagat cccccggctg cggcggcacc
    33181 ccgtcccgtg ctccttgcac gaaactgctg aattgtccgc ctttctctgt ggcacagcgc
    33241 tggccgtaga ggttggtcgc taccgcaaag gcttggggca agggagcgat ggcaccggcc
    33301 agctgttgga atggcgcttg aatgttaatc gaaccgctga ttccagcagg ccctgctgtc
    33361 gcgtcgagca cgctccctgg ctcttgaagc accactccgc cgataatggt gatggcacct
    33421 ccctgccctt cattagcctt ggctagtatc tgggagttgc cttgcatgat gacaaattga
    33481 gggtgcgccg tgtcgatggt aatgttgcca ccgcttgatc ctgccaggcc agctacggag
    33541 ctcgtgagat tggcgccgac gagccggacc agattcggag ccttgagatc gatgcttcca
    33601 cctgacgcct tggcggcaga tgtcgatacc gtactattcg cgatgaagat gttagtccct
    33661 gaggtgagag tgatattccc agcatcattt gctccggtgg tgatggttga gacgagggac
    33721 ccattgttga tcgcgatatc gcccgattgg attgcgatgg tcccaccctt tccgctctca
    33781 gcggtgctcg tgagctgcgc gttcgtaagc cccaccatac ttggcgccac gagtctgata
    33841 cttccggcgg acgcctgagt agcagaagtc gaaaaggtac tgttctcgat gacgacattc
    33901 cccgatccca caaggatatc gccggcatgc ccagtcccag tgcttgtact agttatcctg
    33961 gcacctttgg atacagcaaa actgccgctt ccttggaaga gaatagcacc ggcgaatgcc
    34021 cccccactcg tgtcagcctc aatgtgcgtc ccattttcca ggctgatcga tcgagccaaa
    34081 atggaaatgt gcccgcgccc tcgaataggt tcagaatctc ctcccctgag gatgatactg
    34141 aggctgctat tgtccagact gacgctctcc gcaaaacttt cccccggccc ctcaatcaca
    34201 attcccccgg caggtccagc cgacgtgcct gtgcttgtac tactgctact gatcttcgca
    34261 ccatttgacg tggcaacatt ggccgcgtgt agggtaatgt ctccggctga tgcatccccg
    34321 ctcgtgtcag ccgtaatgcg cgcaccattt actaaacgta tcgtttgagc tgagaggtca
    34381 atattcgcgc gggatgtcgt aagggtgccc ccactgatcg tggtactgat gctgctattg
    34441 tcgaggctga tgttcgtcgc aggaccgctt aaaattcctc caatcaggat ctgtcccgcc
    34501 gatccggccg acgtgcccgt acctgtactg ctactagtga tcatcgcacc attggatgct
    34561 gcgaaatcag ccgtatcgag atgaatgttt ccggctgacg cctccccact cgtgtcggca
    34621 gtaatgcgcg agccattcgc cagacgtatc acttcacccg caaggtcaat ggtcgcaggg
    34681 acagttacag ggctcccacc gctgatcgtg gtgctgatgg tgctgttgtc gagagtcaca
    34741 agattctcag cactacctgg cccatttgtt cctcgtatcg tgaccgtacc ggcactgccg
    34801 tctcccgtgg aggaggcatt aatgccggag ccctttatga gttgaacctc gttgccttga
    34861 atacttatag gtccgcctgg tgcagctgca atgagggtgg atccggaaga gaaatccttt
    34921 ccactcgctg cactcgtgag gttggaaccg tttgtcagag ttagacttcc gacatttaca
    34981 ataatcgaac cgctctgtcc tgcgcctcca tgagtaactg aaacggtgct tccatcaagc
    35041 agcacattgt ttccctcaag gataatggat ccactatttc cgaaaacacc tagattgctg
    35101 cccgcctgcg tcacaaggct ctcaacggtg ctcttaacga gcgtgattgt ctctgctttc
    35161 acgccatctt gcattccagt tatcttgata ctgcctgcag taccagaacc aggcactgga
    35221 gtcccgccta gtccattcgt gctgaaacga gagcctgtaa tctctgtttc atctgcacga
    35281 atgacgatat cgccacctcg cacgatagtg tcaccgttgg cttccactgt tgtgccgctc
    35341 attgatacaa ccggggcctt aatcaccaca tctcccttta tgggcagcgc aaagagacct
    35401 ccaccaagct taccggcaat gaagtcaccc tggcccagcg aaattcgttt cccagctgta
    35461 agctcaatat cgttctgatt cgcacgaagg ttttggacac taccgtcctt ttcaaaagtg
    35521 tttccaccgc gactaaagag ctcattctca gcaaaaacgc tgacgatccc ctgagtcgta
    35581 gacagacgac tatctgcaat atcaaggttg ttagtttgaa tgctgacatc cctcccaagt
    35641 acgatgcagt cacaatcgct cgccgtgccg gcggtgtgaa gcaagctctt attgctaatg
    35701 gtgacttgat ccgcgagcga ctgggcgccc tgcattccgc gaatctgaat ctgtccggtt
    35761 gcactacctg caagactgga aaccacttgc tggccgtaac tcactatctt tgcgttgtcg
    35821 agagtgacat ccgacgaggc aagtgtaata tcgccttggt tattggttaa aatcgaaaga
    35881 ccggacgctt tcgatgtgcc gggatcgcgg gcaaacactg gaaatgtagt accaaatccg
    35941 agttccgtct gaaccgcacc gcccttgatg acaatagtat tggagagaat gtcgacaatt
    36001 cctcccgata attgaggcac gaatgggccc ggagaaggtg cagtggttat cgcttcaata
    36061 cctgcattct ccatgacgaa ccggccactt cgaatcaaaa ctggaccagc atcgcctgtg
    36121 ttggaactcg ttctcaaaat cgccccttct gtgagttgaa tcgtccctag tttggacaca
    36181 ttggcgagac caagagatgg cgtctcacct agctgattgg gaattacctc gccggctcct
    36241 gccacactcg ccaggtttat ctgtccaccc ggtgcggaaa cagttcgtcc agttatcgaa
    36301 atatccccgc ctacgaggga aatcgtcttt ccatcgctga cggaaagtga agttcctgct
    36361 tgtacgataa taggacctgc tgggcgctcg cctaaaaatc caaatgccgt gacagctgcg
    36421 ctggtcagtt ggctcgtttt gcctaaatcc gcatagaagc gatcattgcc gctgccgagc
    36481 cgcaaatagt ccgccgtgct gaaatgcacc gaacccccga cattcagcga ggccgagggg
    36541 ccgaagacga tgccggccgg gttaatcagc cacaacgccg cagttccaaa ccccgccgtc
    36601 tgaatcgttc cataaatatt tgaggtctgc cctccagtga cccggctgag aatgttcgtg
    36661 gtagagcggc cggcctcgtt aaaaaatcga gccacgttgt tcgtcccgac actaaagtcc
    36721 ccaaagctat ggaacagatt gggtccgtca cctggcctag tcccgcctgt aatgatgtaa
    36781 tttggcctac cggcagggtc gatgctagcg ggtgagatcg tcgtgcccaa cccagacgac
    36841 gtaatcgagg tggtctgccc ctcgctgacg gtcgcccata ggagaggcgc taggcctagt
    36901 aggaagctca gaaggcacac ggctcgccac gaactatggt tctgggccat ctgaatcctc
    36961 ttcctcgcag atgagccatg cagcatagta cttttgctgc ctttgacacc agcgaccttg
    37021 aatcccgtaa acgagccaac cgttggcgca ccaaatcctg agaaacttag atcacggttt
    37081 cttcctatac caactcccac accgggcctc ctgcatcttt gtatttcaat ccctgtgcct
    37141 aacaagcgag gttcagccaa ccatccaagt gatgcgcaaa tagacggtta caacaattct
    37201 tcagagagca gaacgacgac accaagggca agatgctaca gatgtggagt caggctctga
    37261 ggcaggaagg ggcacatgat ctgaattgaa ctctgactaa agaagagtaa ccttcactca
    37321 tcacctcgcc ggcggttaac aggccgcacg ctcgtgaaat gcacgtattc tccacagtcg
    37381 gcctatcact gctgccgact cttcctgaac ctgggccaaa ttcggcagac ggccagcggg
    37441 ctctgcttca cattactctg gcttactttt ctggttttgg gtcatcggcc accacccggc
    37501 ggcatataca tcatgcagtt tgggatccag gagcatagct ttccattaaa ccactctaat
    37561 gacttttgac gagcaagccc tccattgggg tcattgaagt atacgatctc cccctcaaca
    37621 ccggtaagaa caattatatg ttcggggcca tcccacactc ctgcacacca aatcgggcca
    37681 tgctggcgca atagcatctc tagatctgat gccgtaaaat ccgcgagttg aggaggatta
    37741 ctacacgatt tcatgccttc ggcttccgcg agtttaataa aatcattcga ctcgagccca
    37801 ttgtttttta tccatacgtc gggaagtcct attcgcggcc ctgctgtgcg ataccatgcg
    37861 atcatgcaaa cgcacgcata ccaacattcc cctgtagcga actgtttcac taatggaaca
    37921 ttcaatctta ttcccattgc gctcctccga acctccctga acacagctgc cgtctgcgaa
    37981 tgatgacctc gtatgtatac ttatgatccc aacggattca ttttgacctg ccccttggtt
    38041 tctgccaaac catcttctcc atggaatgtc accgcggagc aagtatcagt tcagtttatc
    38101 taaagaaaca gccttcggag aggaggacgg acttgatggt ttgcatcgca cacaggacgt
    38161 agacatcgag agatgattcg aacgtacctc aacgcgatga gttcttggtc gatcttattc
    38221 aataccttgc agctcataaa cgggcctacc tttcgtgaac catagtcggc gttcatggct
    38281 gcctccagct tctgatcaca tgcccgcaac tcgtagggtt catcctgcag tgcacgcccc
    38341 tgacactagt ggactgtaac ggttaaaatg gtagtatcat gacgcctatg gaggacgtga
    38401 tgaaactgac caagagtgaa cgcctggaac ttcagcgcca agcgagagcc agcaacggtc
    38461 gagccgattc cgctcgacac gcccgtctga ttctgctgct ggccgatggc ctgacgtggg
    38521 ccacgattcg ggccaagctg gactgcaatg acagttacat cagccgttgg agccagcgct
    38581 ttgccgcgga tcggctgtcg gggttgttcg cccgctacgc cgggcgggcg cgctacaaag
    38641 tgacggatcg gattgaggca cgcgtcctgg cctggacgac gaaacacgca ccggatgacg
    38701 gatcgacgca ctggtcctcg cgcaagctgg cggcggagtt ggggggcatt tcccacatga
    38761 cggtgacgcg catctgggcc aagcacgcgc tgaggccgca tcggctggaa gggtacctgg
    38821 cctccaacga tccggacttc gaggccaagg cggccgatgt gattggcctg tatctgcatc
    38881 cgccgcagca cgccgccgta ttctgcgtcg acgaaaagac ggcgattcag gcgctcgatc
    38941 gcaaagaccc ggtgttgccc ctgtcgccgg ggcgggccga acgccatggc tttgagtacg
    39001 tccgtcacgg caccctctcc ctgtacgccg cgttcaacac caagacgggc gaggtgttgg
    39061 gcaagaccgc accgcgccac acctcggccg agttcgtcgc atttctgacc gacatcgtga
    39121 ccaaccagcc caacggcaaa gagattcatg tgatcgcgga taacctctcg gcgcataaga
    39181 ccgcgcgggt cgaggcgttc ctcgcccagt atccaagggt gcatctacac ttcaccccga
    39241 cgtactcctc ctggctcaac caggtcgaac tgtggttcgc caagatcgaa cgcgacgtga
    39301 tcgcccgtgg cgtctttacc tcggtgcctg atctcaaacg aaagctcatg cgctatatcc
    39361 ggcactacaa tacgcagccc aaacccgtga agtggaagta cttcgatgcg tcacgtcgca
    39421 ttactcccga atcaattgtt acagtccact ag
//
LOCUS       DAORRQ010000042        37472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_69_length_37472_cov_20.426731, whole genome shotgun sequence.
ACCESSION   DAORRQ010000042 DAORRQ010000000
VERSION     DAORRQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 37472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69_length_37472_cov_20.426731"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK224_13575"
     CDS             complement(<1..556)
                     /locus_tag="PK224_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16820.1"
                     /translation="MSIFIHVPAGLTHHETSGYSVVMAFWCKVVLLVSLALCQPATAL
                     VFAAATPEQPESVPADEYPFYDLAVEAKFLTSRTRLVIIERMTSTRLHPEEPQLPTMA
                     WFAEQGFFDGRLPQDLIRDFVAKAQRPARLDARFTFGVRYRFVSGEGVPETEAAVPVV
                     PAAWPIEENGDEDALETLDRLAFSR"
     gene            745..1356
                     /locus_tag="PK224_13580"
     CDS             745..1356
                     /locus_tag="PK224_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16821.1"
                     /translation="MNSLQDGNKVMQQQIDLLNQELRDAKKQAEHAQAEAKALQAEAK
                     ALGTKLDSQLAESKKLSGEIQRTAAKAAQATDNIRVEEKGSQVEDLPRPLAAVGKAAE
                     EALARNGYRVKVSVKTDQKTIYVTERKVTTPTSLETSGSRNQYVVSLQALPSNVTRLS
                     VKADFERLAQGGRILAVDAEETAEIERRLIAEIGKAVASPSKL"
     gene            1356..2300
                     /locus_tag="PK224_13585"
     CDS             1356..2300
                     /locus_tag="PK224_13585"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB16822.1"
                     /translation="MPARSVLPGMASIPRYVGLAILFLASAWSSPSFPATNRAPSPPP
                     SAELAKHLTSIAKLTAGSPTIHIPEGPFLLGSVRVDDDPYGMGTQFDDTELPQHRVWM
                     DAYEIDRDEISLGEYLSYLFAHKLHPSKDLQHLIWHVITVHSVTDETLAQWPALYVTW
                     KEADSLCRSLGKRLPTEAEWEKAARGPDGNRFPWGDTLPDSTLAMFGQHHVHEIPILA
                     PVNSGEAGKSYYGIHHMAGNVAEWVNDWFGFDYYAYMPERNPPGPTSGRYKSLRGGSW
                     KSRPIMLRTATRSGAPADQRSATVGFRCAKSVPPSAAR"
     gene            complement(2237..3421)
                     /locus_tag="PK224_13590"
     CDS             complement(2237..3421)
                     /locus_tag="PK224_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16823.1"
                     /translation="MLQRDAISGSPDIHGATIAWVVAAIVTLPLLGVILGGRAIHPYL
                     EFPPITRSVTHEPFSGIVFIQVAVLIVVILVPFLVRVMRTTRQESRPPMERFPWWGWL
                     AVLWTLIAWLLAWTRFEWMQSLQAHTFTPLWLGYIVLVNALTFRRTGRCMLLHRPRYF
                     LSLFPLSAAFWWFFEYLNRFVQNWHYVGGGELSAWEYLVRATLPFSTVLPAVLGTAEW
                     LSSFPRLSAGLDRFVTFEFKNHTTWGWGLLIGSAVGLFGIGLWPDYLFSLVWVAPLLL
                     ITSLQLIRDEPTIFNDAANGDWRTLWAAALAALICGCFWEMWNFYSLARWEYAVPFVQ
                     RFKLFEMPLLGYAGYLPFGLECLAVADLCLSRKFSGGVAYYRAAEGGTDLAQRKPTVA
                     ER"
     gene            3654..3947
                     /locus_tag="PK224_13595"
     CDS             3654..3947
                     /locus_tag="PK224_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16824.1"
                     /translation="MMKSLILAGLLILPGIAGPLMVPTESLASLAYTRVRIEAVDIAT
                     PSLTFKTMEGQVWTLQATSAEIRQGLHKGDTCSVEIDLENRVTKIVKVDSTSP"
     gene            4011..4505
                     /locus_tag="PK224_13600"
     CDS             4011..4505
                     /locus_tag="PK224_13600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRB16825.1"
                     /translation="MSKKLPVMFFAALAAMILLNRPVQAQTGSPSIEEGAKVTLQMTI
                     SVPNDHLIIPPHTSEYTHGAKQLIPGLEQALVGLHAGEKKHVELDADHAFGQYDASKK
                     STIPRAQLPQTITAGEITTTPEGRPFTVVSLAEDSAVIDFNHPLAGKQIVLDVVVLKV
                     KPRT"
     gene            4681..5322
                     /locus_tag="PK224_13605"
     CDS             4681..5322
                     /locus_tag="PK224_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16826.1"
                     /translation="MRRSLTHPSILVMAIIAVQCWLTPLVHSQGRSAQVTVTPTADKV
                     IAWSAAGGGVEESLQPGESVVASGARGHTGFVQTSTRLLGFSAGLRQWREVSLGVEER
                     IGRHQLLPFLILAHSNQQVYGFQETRGHWTTLSLGPSEKVVQLRGHGHVAVAITNERA
                     LGFSTYTGGFFNIAWTPEERVLSVDGSQNGMVVRTSSRTVIFKSQSTGWTEVR"
     gene            complement(5350..5787)
                     /locus_tag="PK224_13610"
     CDS             complement(5350..5787)
                     /locus_tag="PK224_13610"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRB16827.1"
                     /translation="MTQQTYPRSPKVLLGGIAHLGRFIDKVRLRHAGKIQDYNYITVG
                     FDKYLIDFLQIDPKAFERQVLAGESDEKLLNWVKTNGRKHSDQEIAEWSKGILTGGPK
                     DEAAKQRYQGRLQDIATKRGVPVTALPPATTWVDAIELDEGRM"
     gene            complement(5790..5981)
                     /locus_tag="PK224_13615"
     CDS             complement(5790..5981)
                     /locus_tag="PK224_13615"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRB16828.1"
                     /translation="MRTTIVIDDSLMRQAMCATGLLPKKTVVEEGLRLLVKVHRHRSI
                     RRLRGKIVFDGVLNKKGMK"
     gene            complement(6120..8057)
                     /locus_tag="PK224_13620"
     CDS             complement(6120..8057)
                     /locus_tag="PK224_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB16829.1"
                     /translation="MNIRLTHLVIRTRQTTEKIHFSETVTFLYGPVGTGKSTVARLVD
                     YCFGGDLERTPAIQQEFVSVILGAKLGEYECTFERGAEDTQAVRVTWTSGEDDFGSVE
                     APLSAGEAPLLDAEVYSLSDLMFHLCDVEPIKVLKRSRDPDSPMVRLSFRDIWWYCYL
                     DQTHLDSSFFRLEDPFRGRKSQDAMRYFTGLHSERLSQLQADLYRTVTEQHAARESVA
                     QIRRFMARFDLGSELELTAKIAATEREMQAAEERKQTLEADRVTTIHPTDKLRSRLRE
                     LSRTVSDLREAISASQSTLAEQEALRAELITAKVKAGRAEQAGRILDGIDYSYCPQCG
                     SDVSGRPPAVEQCRLCGTPSTAINDRPSVDAESVRRELNDRIDQIADSMIRRRQALER
                     SERGLQKAESTKRQLDRQLQEELRRYDSAFVESIRVSDREIATLEERLRSLVQLQEMP
                     RAISELEEQAGASQGRIDRLRSTLDEERGRLRSADSNARAIAERFKAIMLEVGFPGVS
                     DEDDVVLDPRNWEPIVVHSRQEWGFWDTGSGGKKTLFNVCYALAVHEVARERGMPVPN
                     ILIIDSPTKNISDEENPELVQSLYREIYRFAADGNGAGTQFLLIDSDLVEPEVELTGF
                     SQRRMAAEPEAPSLISYYLGP"
     gene            complement(8054..8617)
                     /locus_tag="PK224_13625"
     CDS             complement(8054..8617)
                     /locus_tag="PK224_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16830.1"
                     /translation="MGVAVMDLVAMIAALDAEPSLHAARLLVLISAFSGTGQGDAVEG
                     LTKLAKLDFLLRYPVMLERALAAKGRSTRDVQLEDHERLSVESQMVRYRFGPWDHRYR
                     EFLNILVGKGLVTVSMEGRKVVISLTERGRALAFELSASPAFQDYARRSSLLKRHFDV
                     QATTLMQFIYETFPEVVSLRSNERIPA"
     gene            complement(8602..9843)
                     /locus_tag="PK224_13630"
     CDS             complement(8602..9843)
                     /locus_tag="PK224_13630"
                     /inference="COORDINATES: protein motif:HMM:NF025495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dsDNA nuclease domain-containing protein"
                     /protein_id="HRB16831.1"
                     /translation="MAAAPIPSPENTVSGGDPGDETANRYRYQWTWAAVVCCMLLDDT
                     EDVEEIFCEHHEDVLLKHRDGWFTGHQVKTRRDDQPPWRAKDEAVLAAFVRFAKLESK
                     YPGKFRRFCFLTNHPLYSAGNGQDLPHILQSIKDAKSIANLPSIVAPWVRRVARDADV
                     PETVAFAALSKAVAKADLPKLRDAVMRLIDAIAQCWASAADCSHDAVKRAAHALIDEC
                     GRASSLDHEQLLPAYMIAALDDDAAMAARIDGKRMTLARVQSILEHGRNFTATLAGDP
                     ILCVEPGEGSTELLHKKLDAGGFSAVSRNSAEDLRDKADYLGIRWTKKYGREKGLQRY
                     DHVRSAVLSDASRAFDASQTKADGFGPAMREDLRRRFRERRRSGDQLFDSTDDHLEGI
                     AFSLTAQCKVVWSHARPWESQ"
     gene            complement(9954..10172)
                     /locus_tag="PK224_13635"
     CDS             complement(9954..10172)
                     /locus_tag="PK224_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16832.1"
                     /translation="MDSRLSDLSNVTQPEGWNAADQNLLVAALSAMPAQRLAWLEETL
                     HLAYASGALKTRALITQDDWGAASSRSR"
     gene            complement(10448..11881)
                     /locus_tag="PK224_13640"
     CDS             complement(10448..11881)
                     /locus_tag="PK224_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRB16833.1"
                     /translation="MGDPKGFLKYAREGPKRKPVELRVLDWKEMYEPIAEDKLKVQGA
                     RCMDCGVPFCQGNTGCPVVNLIPEWNDLVYRGRWKDALKALHTTNNFPEFTGRLCPAP
                     CEGACVLGINSDPVSIRVLEWNIIDRGFNEGLVEPAMPVRKTGKTIAIIGSGPAGLAA
                     AQQLARAGHTVTVFEKSDRIGGLLRYGIPDFKMEKWVIDRRLEQMKAEGVEFKTGVTV
                     GKDITGEQLRQQFDAVGLTMGAEMARDLPVPGRELKGIHFAMEYLTQQNKRTAGISVS
                     EEPITAKGKRVIIIGGGDTGSDCLGTAHRQGCSEAHQFEVLPEPPPTRASSTPWPLWP
                     MQLRTSHAHEEGCDRQWSVSTTKFTGHNGHVTKLHANRVKFEGGKFVPMPNTDFELDA
                     DLVLLAMGFTGPVRNGFLDSLGVTYDARGCVTVDDNFMTNLDGVFAGGDTKRGASLIV
                     WAIAEGRKMAAGVDKYLQANKSAKTGR"
     gene            complement(11968..16488)
                     /gene="gltB"
                     /locus_tag="PK224_13645"
     CDS             complement(11968..16488)
                     /gene="gltB"
                     /locus_tag="PK224_13645"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRB16834.1"
                     /translation="MNVPGLPPKQGLYDPQHEKDACGVGFVVDIKGQRSHQIVQQGLQ
                     VLESLTHRGAQGCDPCTGDGAGILLQVPHEFFKRAAKDCGIKLPGAGEYGVGMVFLPP
                     DADARVQCETVFNRVIKDANAKLLGWRDVPVKSDAIGELARTTEPFMRQVFIARGIFT
                     DDEFERRLYVIRKCAERAVRESAIEGREYFYIPSLSSSTIVYKGLLLPHQIPQYYQDL
                     TDSSVTSGLALVHSRFSTNTFPTWPLAHPYRYICHNGEINTLKGNVNWMRARQGRLNT
                     ELFGEDMQKLFPIVYENQSDSACLDNALEFLVLGGRSLPHAMMMLIPEPWVANPQMDL
                     DRRGFYEYHAAMQEPWDGPAAVCFTDGKLIGATLDRNGLRPCRYQVTTDGLVVLASEA
                     GVLPMDPQRIRQKGRLMPGRMFLVDTVQGRIIDDEEVKADIVRRKPYRSWVAQYRISL
                     DELPDPINVPQPDHPTIRQRQQAFGYTVEELKMVITPMVVEGQEAISSMGTDTPLAVL
                     SDRPQLLFKYFKQLFAQVTNPPIDPIREELVMSLTTSIGPKPNLMDEHPESCRRIRVK
                     QPILTNADLQKIREINDPNFKSKTLKMLFRVAEGPEGLGAAVDDLCRQASQAIREGYK
                     FLILSDRGVNADYAPIPSLLGVAAVHHHLVRECTRTEVGLTVETGEPRDVHHFACLIG
                     FGAGTVNPYLVFESLVDLERDGYFPEGLDAPTAEGKFIKAINKGLLKIFSKMGISTVQ
                     SYCGAQIFEAIGLNHELIDRYFTGTPSRIEGISIREIGEETLRRHRVAYEPAPIRQLD
                     FGGEIHYRIQGEHHNWNPDTIYKLQHATRNNDPKTFAEFSQLVNDESRRRSNLRGLLE
                     FKFQPEAIPLDEVEPAKDIVKRFTTGAMSFGSISKEAHETLAIAMNRLGAKSNTGEGG
                     EDPERFAPLPNGDSRNSYIKQVASARFGVTSHYLVNAKELQIKMAQGAKPGEGGQLPG
                     HKVDENIARLRYSTPGVQLISPPPHHDIYSIEDLAQLIFDLKNANSDAAVSVKLVSEV
                     GVGTVAAGVAKAHADKVLISGDSGGTGASPLSSIKYAGVPWELGLAETHQTLVLNDLR
                     GRIRVETDGQMKTGRDVAIAALLGAEEYGFATAPLIIEGCIMMRKCHLNTCPVGIATQ
                     DPVLRKKFTGQPEHVVNFFFFIAEELRQIMAKLGFRTINEMVGRVDKLKIHKAVEHWK
                     AKGLDLAPLLKMPEVGPEVSRYCVQKQDHGIADILDRKLIEQCRPAIDRGEKVTLELP
                     IRNLNRTVGTMLSSQIAKKYGLEGLPADTITIKFNGSAGQSFGAFLSRGITLVLEGES
                     NDYIGKGLSGGKIIVFPPKNAIYTPEETILVGNTSLYGGTQGEAYFYGMAGERFAVRN
                     SGVRAVVEGTGDHGCEYMTGGVVAVLGRTGRNFAAGMSGGVAFVLNELDKFQSRCNLG
                     MVELEQVTSDDDKKLLHDMITSHFMYTGSRNAKRILDGWEVILPKFVKVMPIDYKRVL
                     AERKAAAAKVAK"
     gene            16726..17730
                     /locus_tag="PK224_13650"
     CDS             16726..17730
                     /locus_tag="PK224_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRB16835.1"
                     /translation="MSNGPTTLTLHSMADVWDVYREELEGVEEQIRKNLDSSVALVNT
                     VAAHILNSGGKRVRPLFVLLSAHLCGYAGQDHQALGSLVEFIHTATLLHDDVVDDADL
                     RRGRRTASKVWGNQISILVGDYLYSRAICQIVDFRNQGINEALSEACRKMAEGEVLQL
                     YYNGNPLMPESEYLRIIEHKTAGLIAASCKIGAIVGGATEELQEALFRFGQRLGIAFQ
                     LADDTLDYTANGEHLGKTLGQDLRQGKATLPLLHLLQHCSEPDRQLIKDRMETRTLTD
                     EELRRIVGLMQEYGSIAYAMERARAFVAAAKRDLDLFHDNTAKRALAIAADYMVTRDR
                     "
     gene            17860..19182
                     /locus_tag="PK224_13655"
     CDS             17860..19182
                     /locus_tag="PK224_13655"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRB16836.1"
                     /translation="MAHVIPFHGTLYNPATVGDVRQVVAPPYDIIDATLQKTLHDRHP
                     NNVIRLELGYEQPGDTTGDNKYLRAAGALKEWLKSGALRRDDKPAIYYHTIEYQPPYS
                     APGTPTKVFKGFLSTVELEEFGSGKIYPHENTRAAAKTDRFNLLEACRANFSAIISLY
                     SDPQNDVLTLIEQSIASDKPRIDFQDDVGFRQRLWSVTDPAVLAKVVEIMHTKQLFIA
                     DGHHRYETALNYRRARRQQAGAPSGPQPYDNVLMLFASLEDKGLTVLPTHRVLTTGVP
                     APKDLLRMLDPVFEVTTLPFQAGNEAQVRGQFIETLRSRGQSVPMFGLALKNDPQYYL
                     LTLREAHRPTASASPRDRLDVSLLQQHVVATLCPTQQEQEAMLYSKDDHEALNWVRQG
                     TGTAALLLNPTKVAEVKAVASAGERMPHKSTYFFPKPLTGLVMNVMEG"
     gene            19229..20605
                     /locus_tag="PK224_13660"
     CDS             19229..20605
                     /locus_tag="PK224_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB16837.1"
                     /translation="MKAKVLIVDDDQDIVTMLQDRLDAGGYKTLTASDGLRGIELIEQ
                     ESPNLVLLDLYLPRLKGMDVLKRMAQNKQSEDIPVIVMTAAGTIPDAVEAMRHGAYDF
                     LTKPLEKDHLLIVIQKALERDSLKRQVAALQSDVQNRYATIVGDSPKIQTIIESARRA
                     ANSDASILLLGESGTGKELFARSIHQWSPRQSMPMVVINCVALTETLLENELFGHERG
                     AFTGADRLQKGKLEMAEGGTVFLDEIGDMPLPLQAKLLRVLQDKEFQRVGGTRSVSVN
                     IRFVAATNKDLKQAVKAGQFREDLFFRLNVVSFTLPPLRERSEDIVRLAEFFLKRHAY
                     EAKRPGVSLSPAARAALTHYPWPGNIRELDNVLSRAVVLSPSDVIEPEFLALSPDDAA
                     GKGGSEHGLPYLNLTYHESMEAHSAYIIERALEKAAGNQTKAAEFLDLQRTYLARLIK
                     QRKSTPDE"
     gene            complement(20888..21622)
                     /locus_tag="PK224_13665"
     CDS             complement(20888..21622)
                     /locus_tag="PK224_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRB16838.1"
                     /translation="MNAMILAAGLGTRLRPLTDVTPKPLLPVAGTPMIVWNLLLLKRH
                     GIREVVINLHHLGAMIPQALGDGSAFGMRLIYSNEPVILGTGGGIKQAEPHFHGEPVL
                     ILNGDTLFELDLGALIAFHVAQQAAATLVLRQDPEAARWGLVEVTDRGEVVRITGRGR
                     TETIPTTGRMFAGIHILHPRLLRAVPVGKESSIIDAYVRGIQEGERILGYDFSGYWSD
                     VGTPERYAQAEQDAAAGLLRLSARKS"
     gene            complement(21763..22863)
                     /locus_tag="PK224_13670"
     CDS             complement(21763..22863)
                     /locus_tag="PK224_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRB16839.1"
                     /translation="MTTTNPAAPAAPLAPPDLARIAKTVATKLPFRGDVTGLTPLAGD
                     ASNRRYFRIALKGTPSALILMQLADPEGFKKSEEAVSGASVQIAELPFTNVLAHLQRT
                     GVTVPQLHYYDREAGLLYLEDFGDLTLAEACRDADRARLVSLYRQAIDQLVLLQVKGT
                     QPPAPGCIAFHRGFDVPLLMWEFDHFLEYGIVARLGQPMKPADDKAVRAEFQRIAELL
                     AGQPQVFVHRDYHSRNLMVDGSRIGVIDFQDALMGPATYDLASLLKDAYIELDDAVVD
                     GLVDHFLDGLAAQGQGWADRAAFRRLFDFTSIQRNLKAAGRFVYIDRVKHNPKFLADI
                     PRVLGYVKRNLSKYPELATLRQHLAPYVSELQ"
     gene            22998..23969
                     /locus_tag="PK224_13675"
     CDS             22998..23969
                     /locus_tag="PK224_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRB16840.1"
                     /translation="MHYVHLGRSGVRVSRLCLGTMNFGPQTSETDSFAIMDQALERGI
                     NFFDSANVYGWKVGEGVTEQIVGRWLSQGSGRREKIVLATKVYGRMGDWPNQSRLSAL
                     HIKRACEESLRRLQTDHLDLYQMHHIDRECPWEEIWQAMEQLCREGKVLYVGSSNFAG
                     WHIAQAQEMAKSRHFLGLISEQSLYNLLERTVELEVIPACQAYGIGIIPWSPLARGLL
                     GGALSTHTTGRRTDEDVRKYIETHRTKLEAYEQFCKTVNATPAAVALAWLLHQPAVTA
                     PIIGPRTMEQLTSALAALELMLTPEHLKTLDTIFPGPGGAAPEAYAW"
     gene            complement(24095..24538)
                     /locus_tag="PK224_13680"
     CDS             complement(24095..24538)
                     /locus_tag="PK224_13680"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRB16841.1"
                     /translation="MESNQMVSREIRTAPLVSPFSVLRYWGPVCVYAGLIFFGSSVSN
                     PPESVSSLMEKISDKILHLCEYGVFGALAYRACRHGAGAWVARHAVVVAVAGCALYGL
                     SDEIHQLFVPLRQGDPLDLVADSVGAALGAWTWRLMERRAVQSSL"
     gene            24696..26900
                     /locus_tag="PK224_13685"
     CDS             24696..26900
                     /locus_tag="PK224_13685"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRB16842.1"
                     /translation="MKTIQLCFLWHMHQPYYTDPLTGSASMPWVRLHATKAYFDMAFL
                     LERFPEARSTFNFTPSLLLQLEEFSTGRVRDLFLEYAQRPAAELTPTEKAFLIRHFFS
                     ANWATMVRPFPRYQELLVKRGVDVHGQDLDRLARQFSTQEFLDLQVWHNLAWFGYGSL
                     QRFPRLAELRKKNRGFTEEDKQEVLALQQTAIRQIVPMYTALQERGQIELTTTPFFHP
                     ILPLVIDSEFTRRARPDLPLPARFHAPADAEAQVRRAIDYHTHTFGRAPAGLWPSEGS
                     VCPELLPILGKTGIRWLATDEGILYRSLQMADQTWNRHYHLYQPYAVGTAEQPLTMVF
                     RDRDISDAFGFVYHKTTPESAADDVLRRIRGLAYDIPLENGLLAVILDGENPWEHYHD
                     GGERFLSLLFRAFEQDGLHIGHGIRVRLNTVSQALESVPPPQRLDQLHSGSWINQDFK
                     IWIGHQEDNRGWDLLQHTRARLVDLTPSLAQDKARAAWDELYAAEGSDWFWWYGDDFD
                     TDYKQEFDRLFRTHLRNVWTYAGVTPPDILNQPLVEARTPQGLDLVQLPLALITPTLD
                     GMVSNFFEWRGAGTINPTPPLGAMWKSEGLFTSIFFGFDREHLYLRLDLDERTQTRQE
                     HCTADLFIGSGIQQYKLSFALTAEGADTFLLSRADESGLYRDRGSYRTICRRKILELG
                     IPFKELGIEIDTELRLTLTVSEHGMEIARYPHHSPATFNRPGDDFEATMWRV"
     gene            27004..28005
                     /gene="galT"
                     /locus_tag="PK224_13690"
     CDS             27004..28005
                     /gene="galT"
                     /locus_tag="PK224_13690"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRB16843.1"
                     /translation="MPELRRDPIVGRWVIISTERGGRPQDVSMPSAPLPSASMCPFCP
                     GQERLTPKEILAYRPHASEPDSPNWTVRVIPNKFPALHVEGDMGREGLGLYDRMNGIG
                     AHEVIIETPNHKENLADLPAKRVEDVLWAYRDRILDLKKDLRLRYILIFKNQGATAGA
                     TLEHSHSQLIALPVVPTSVLEEIDGCRQHFQQKERCIYCDILRQESSEGTRVVLENPE
                     FVCLTPYAPRFPFEMWILPKRHAGYFEECQRAQFEFLAPILGEALRRMDAVLARPAYN
                     FILHSSPLHEKTGDYYHWHIEIIPKLTQVAGFEWGTGFYINPVAPEEAAKALRDAEI"
     gene            28313..28465
                     /locus_tag="PK224_13695"
     CDS             28313..28465
                     /locus_tag="PK224_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16844.1"
                     /translation="MKAHDLEWKDETFSELDLQNQKFKRLAIGSALLFLSVVGLLIYQ
                     IIAGIK"
     gene            28631..28825
                     /locus_tag="PK224_13700"
     CDS             28631..28825
                     /locus_tag="PK224_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRB16845.1"
                     /translation="MLVHLSHPQRQVEIKGPKRTKELLRELNLVIEAHLVIRGDELVT
                     EDEMLADADQIEIRPVISGG"
     gene            28870..29808
                     /locus_tag="PK224_13705"
     CDS             28870..29808
                     /locus_tag="PK224_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB16846.1"
                     /translation="MNCGKCKTKAVISLPRHNAAFCKGCFTTFVHEQVARAIKSFKMF
                     TPEDRILVAVSGGKDSLALWHILLTLGYRADALYVNLGIGAYSEESHRKVRHYADTVA
                     AAHGAKLIVHVVEQEAGAGIRELATVLHRPTCSTCGTIKRYQFNRAAVEQDYDVMATG
                     HNLDDEAARLLGNVLHWQEEYLDKQSPTLPASVEGFAKKVKPLCRLSEREIAAYAVVN
                     RIDYIVEECPMAKGSKMILYKEVLNRLETESPGTKQRFYWGFLEKQTKPEPATESMAE
                     KDQRTLHPCTTCGQPTTAEICTYCKMMAKAKTATPR"
     gene            complement(29851..30519)
                     /locus_tag="PK224_13710"
     CDS             complement(29851..30519)
                     /locus_tag="PK224_13710"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRB16847.1"
                     /translation="MKRGLRQWFSLPVGVLPRMLVTLAVMLIGSVMMLSEAHALQASP
                     TSLSFQAVQGGPNPSSKTVKVYKSSSRKVSWSGKDNAGWLSLSPTSGTLGSSTQVTVS
                     VNISGLAAGTYSGTVTITTSRGSTVAIPVTLKLSAATSSSTTGTTAALAWNANAESDL
                     AGYKVYSGTSSGQYGASVDVGKATSYQFSNLKLGTTYYFAVTAYDVNGNESLRSSEVS
                     KSIY"
     gene            31139..32533
                     /locus_tag="PK224_13715"
     CDS             31139..32533
                     /locus_tag="PK224_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRB16848.1"
                     /translation="MRRIVRAFFTCSLLQSVPRHPLRANHQRRAALLLLWLFLFGPPL
                     NDSIADGPAAPSKGQTTPITLRFVSWKPDHPRVWDDALDEFTKAHPHISIVRELAPHS
                     STAYHDLLTQKLKNRDATVDVFFMDVIWVPEFAEAGWARRLDERFTPAMREEFLPATI
                     EVGRYSDHLYGVPSRIDAGLLYYRSDLLAKYGFSPPTTWDELARQADAIVAGERRNNP
                     TLRGYTAQFKQYEGLVCNLLEFIDGHGGSLLTSDGTHSTLGRPETLAAVQFVRDRVIG
                     RLASRAALTYQEPESLSVFLQGHAVFHRNWPYAWELANNRTRSTIAGQVAVMPLPGFT
                     PGRTAAALGGWLYGISAYSQHPDEAWALIEFLSSQAMQKKFTQEAGIAPSRQALFSDP
                     DLLAAAPQLRNHFAVLRSATARPRSPLYPAISHLLQRYFSRALAIDGLDLAQEAAVTD
                     AHIDRLLALTRIAP"
     gene            32530..33441
                     /locus_tag="PK224_13720"
     CDS             32530..33441
                     /locus_tag="PK224_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRB16849.1"
                     /translation="MIRPSPILRHRDSLAAWTMVAPALLVTMVFALYPVLDSLWLSLH
                     NIFIGLPQLGSPFVGLDNYVALLRDPVAQQALVVTLAFVGLSTILELACGLIIALVIH
                     ERFRGRGLVRAAILIPWAIPTVVASQLWRYIFNDQYGFANLLLFGERVTDYVPWLAYP
                     GVAFGIVVLADVWKTSSFAALLILAGLQVIPDDLYDAARVDGATVWQRFGHITLPLLK
                     PALLLALLFRTMDAFRVFDLVFVMTQGGPGDATQVLQFYGYQTLFTEGRIGYGSAVSV
                     VVFLMILALSLTYLRAIGSSLLERRRT"
     gene            33438..34253
                     /locus_tag="PK224_13725"
     CDS             33438..34253
                     /locus_tag="PK224_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRB16850.1"
                     /translation="MNRRILLALGILGTVGLSLLPFLWFVLTSFKSQGEIEVAPPAWW
                     PSGNLGFYRSALFDHHLFDYVLNSVVVAGSTTVLALLFAIPAAYALARLTIPGKQGIL
                     AVLLCVSMFPQMAIAGPVWRLLDTIGGLNHRWGVVLPYVALTLPLAIWILASFFKELP
                     PELEDAARVDGCGPWTTLFRITLPLATPGIFTAAILILIYAWNEFFFALLILTQPEQQ
                     TLPVGIALFQGEFTMPWGELAAASVVATLPLIVVVLLCQRWIVSGLSAGAVKG"
     gene            34413..35513
                     /gene="ugpC"
                     /locus_tag="PK224_13730"
     CDS             34413..35513
                     /gene="ugpC"
                     /locus_tag="PK224_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRB16851.1"
                     /translation="MAELELRGVSKSFGDQPVLRDLSLQVPDGSFTILLGPSGCGKST
                     LLRIIAGLDSQTAGQVCIDGTVVDHLPPAERDIAMVFQQYALYPHLSVRENLAFALTI
                     RRTPRRVIDERIAEAAQLLDIEPLLDRKPKDLSGGQRQRVAMGRAIVRKPKLFLFDEP
                     LSNLDAQLRTTMRIELKKLQQRLQATMIYVTHDQVEAMTLGDRIVVIEGGRIRQADEP
                     HRTYAAPADPFVASFIGTPPMNLWEGALMTEGGETVFRSHDLTLPMPARLIPPDSTQN
                     SAVTLGIRPEDIQLRETQTAVPLSGLVELVEDLGADLLLHCRAGELRLVVRTARRTDK
                     IQRQTVKLFFPVDKLHLFIDHRRFDPPTTTPT"
     gene            35672..36700
                     /locus_tag="PK224_13735"
     CDS             35672..36700
                     /locus_tag="PK224_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRB16852.1"
                     /translation="MATSQRGYYDILGVPRNATADDIKKAFRRRAREFHPDLHTGTKK
                     TEMEKKFKELNEAHEVLSDPDKRKKYDQYGSNWEHAEAYEQARQQAGAQAGRGGQAGG
                     FSGDFGDIFETFFGGRTRGGTSPGFAVDGEDLETDVHLTIRDVLTGVSRRIDLTERVV
                     CKACGGSAIVRGRPCVVCGGAGTQAEKRTIEVRIPAGVQNETRVRLAGKGQPGINGGK
                     PGDLYLRVHIQANGVFRQKGFDIQVTLPVWPWEAALGAEVMAPTLTEPVKVKIPPGSK
                     ADSKLRLKGKGLPTSTGEQGDLFLKLKIVMPTAISDEERALYEQLGRARHSDPRAEII
                     AASRRSSS"
     gene            36729..37376
                     /locus_tag="PK224_13740"
     CDS             36729..37376
                     /locus_tag="PK224_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRB16853.1"
                     /translation="MTIAEYALLAFSSLFVIVDPIAVVPAFLAMTPRDSVAQRLRTAR
                     VACLVSVALLTGFALFGQTVLKLLGITLPAVQIAGGLILLLVALDMLRAQRSTVQETA
                     AETAAGTNKDDIAITPLAIPMLAGPAAISTVILLETQATTFMLHVVLLGCLVLIGLAS
                     YIILAIDARSAKWLNPIAEKIISRLMGLLLAALAVQFMVNALTGDQGLLRGLTGH"
BASE COUNT         7847 a        11793 c        10456 g         7376 t
ORIGIN      
        1 cacgggagaa ggccagccgg tcgagggtct cgagagcatc ctcgtcacca ttctcctcaa
       61 taggccaagc agcgggaaca accgggacag cagcttcagt ctccggcacc ccttcgccgg
      121 acacgaaccg gtagcgtacc ccgaaggtaa aacgggcatc caatcgagcc ggccgctgag
      181 ccttcgccac gaaatcccga atgagatcct gaggcaaccg gccatcgaaa aatccctgct
      241 cggcaaacca ggccatcgtc ggcaactgcg gctcctccgg atgcagacgg gtggacgtca
      301 tgcgttcgat gatgaccagt ctggtacgag aggtcaggaa ctttgcctcg acggcgaggt
      361 cataaaacgg atactcgtcg gcaggcacgg actccggctg ctcgggcgtt gccgcggcaa
      421 acaccaaggc tgtcgctggt tgacacagcg ccagactgac gagcaacacc accttgcacc
      481 agaacgccat gaccacagaa tagccgcttg tttcgtgatg ggtcaaaccg gcgggaacat
      541 gaatgaagat ggacaagggg ggccaaactc tggtattggt ctgatggtct tacccatact
      601 atgatcaagc ctccccagcg atcgctcgca gctctcttca gcctcgcagt actatcgtgg
      661 tgtgtcggat gcgcaccgga cgagtcgccg tttcgccaag aaaacgaaag cctccgcaag
      721 caactggcga agcaggagtc ggtcatgaat tcgctgcagg acggcaacaa ggtcatgcag
      781 cagcagatcg atctgctgaa ccaggaattg cgtgacgcca agaagcaggc ggagcatgct
      841 caagccgagg caaaggcgct gcaggccgaa gccaaggcac tcgggaccaa actcgactcc
      901 caactggccg agtcgaaaaa gctgtcggga gagattcagc gaacggctgc aaaagcggcc
      961 caggccacgg acaatatccg cgtcgaagag aagggctccc aggtcgagga cctcccgcgc
     1021 ccgctggctg cagtcggaaa agccgccgaa gaagccctgg cgcgcaacgg ctaccgcgtg
     1081 aaagtcagcg tcaagaccga ccagaaaacg atctacgtca ccgaacgcaa agtcaccacc
     1141 ccgacctcgc tggaaacctc aggctcgcgc aatcagtatg tggtgtcgct ccaagccctg
     1201 ccgtccaacg tcacccggtt gagcgtcaaa gccgactttg aacgcctggc gcagggcggt
     1261 cgcatcctgg cggtggatgc ggaggaaacc gcggagatcg aacgccggct gattgccgaa
     1321 atcggaaaag ccgtcgcctc gccgagtaaa ctgtaatgcc tgcccgttcc gtcttaccgg
     1381 gaatggcttc cattccacgc tacgtaggcc tcgcaatcct gttccttgcc tcagcctggt
     1441 ccagcccgag tttccccgct accaaccgag cccccagtcc tccaccttct gctgaactgg
     1501 ctaaacacct gacatcaatc gccaaactca cggccggttc gccgacgatt catatccccg
     1561 aaggaccgtt cctgctgggt agcgtccggg tggatgacga tccctatggg atgggcacac
     1621 agttcgacga tacggaactc ccgcagcacc gggtctggat ggatgcctac gaaatcgacc
     1681 gcgacgaaat cagcctcggg gaatatctct cctatctatt cgcgcacaag ctgcatccat
     1741 caaaggatct ccagcacctg atctggcatg tgatcacagt acactccgtc accgatgaga
     1801 cgctggccca atggccggcg ttatacgtga cctggaaaga agccgacagc ctctgccgct
     1861 cattgggcaa acgcctgccg accgaagcgg aatgggagaa agccgcgcgc gggccggacg
     1921 gaaatcgctt cccctggggg gacaccctcc ccgacagtac actcgccatg ttcgggcaac
     1981 atcacgtgca tgagatcccg atcctggcgc ccgtgaacag cggcgaggcg gggaaaagtt
     2041 actatggaat acatcacatg gcgggcaatg tcgccgaatg ggtcaacgac tggttcggct
     2101 tcgactatta cgcctacatg ccggaacgaa atccgcccgg acccaccagc ggccgctaca
     2161 agagcctgcg cggcgggtcc tggaaaagtc gtccgatcat gctcaggacc gccacccgca
     2221 gcggcgcgcc ggccgatcag cgctctgcca ccgtgggctt tcgttgcgcc aaatcggtgc
     2281 cgccttcagc cgcacggtaa taggccacgc ctcccgaaaa ctttctcgac aagcacaagt
     2341 ccgccacagc aagacactcc aggccgaacg gcagataacc ggcatatccg agcagaggca
     2401 tctcgaacag tttgaaccgt tgcacgaacg gcaccgcata ttcccagcgg gccaggctgt
     2461 aaaagttcca catctcccag aagcatccac agatcagtgc cgcaagagcc gctgcccaca
     2521 gggttcgcca atcaccgtta gccgcgtcgt tgaagatcgt cggctcatcc cgaatcaact
     2581 gcagcgacgt gatcagcaac agcggcgcga cccacaccag cgagaagaga tagtcaggcc
     2641 acagaccgat tccgaaaagc ccgacggccg aaccgatcaa gagcccccac ccccacgtcg
     2701 tatggttctt gaattcgaac gtcacaaacc gatccagccc tgccgatagc ctcggaaatg
     2761 acgacaacca ttctgccgta ccaagtaccg cgggcagcac cgtggagaag ggtagcgtcg
     2821 cgcgcaccag atactcccac gcgcttaact ctcctccacc gacgtagtgc cagttctgaa
     2881 cgaagcgatt cagatattcg aagaaccacc agaaggcggc gctgagcgga aagagcgaca
     2941 ggaaatagcg tggacgatgc agcagcatgc aacggccggt gcggcgaaac gtcaacgcat
     3001 tcaccagcac gatgtagccc aaccagagcg gagtaaaggt gtgcgcttgc agagattgca
     3061 tccactcgaa cctggtccag gccaacaacc aagcaatcaa cgtccacagg accgcaagcc
     3121 aaccccacca agggaatcgc tccatcggtg gtcggctttc ctgacgtgtc gttcgcatca
     3181 ccctcacgag aaacgggacg aggatcacga cgatgagcac agccacctgg atgaagacga
     3241 ttccggaaaa tggttcatgg gtgactgagc gggtaatcgg cggaaattcc aaataggggt
     3301 gaatggctcg accgccgagg atcacaccga gtagcggcag cgtgacaatt gccgccacaa
     3361 cccacgcgat ggtcgccccg tgaatgtcag gacttccaga gattgcgtca cgctgcaaca
     3421 tcggccgttc cagaatgagc cattgactta cgtttcaatg gacaaaatgt cccgtacgga
     3481 caagatgcgc gtaagaaacg atattttgtt cgcattaaac tcttccaaga agaatgagtg
     3541 taactatgga aaaatttgca aatcatctga caagaacggt cgattgcgga tcaggctcgt
     3601 tgcttgaata actcaaccag tggcttttca ttctgcatca gaaggaggat gacatgatga
     3661 agtctctcat cctggccggc ttgctcatcc tgccggggat agccgggcca ctcatggtcc
     3721 ctaccgaaag cttggcctct ctagcttata ctcgcgtacg catcgaagcg gttgacatag
     3781 ccactccaag tctgacattt aaaacgatgg aaggccaagt ctggactctc caggccacat
     3841 cggctgagat tcgacaggga ttacataagg gagatacctg cagcgtggag attgatctgg
     3901 aaaaccgagt caccaaaatc gtcaaagtgg attccacatc tccataggga ataggagagg
     3961 gggttcgctc tatcaaccac ctgaatgact gattctttat gaggtgtcgg atgtctaaaa
     4021 aactacctgt catgtttttc gcagcattag cggccatgat tctgctaaac agacccgtgc
     4081 aagcgcaaac aggctcgccc tctatagaag agggcgcaaa ggtcacgctt caaatgacga
     4141 tcagtgtgcc caacgaccat ctcatcattc ctccccacac cagcgaatac acgcatgggg
     4201 cgaaacaact gataccaggc ttggaacagg ctctcgtcgg actgcatgcc ggtgaaaaga
     4261 aacatgtgga actcgatgca gaccatgcct tcggccaata tgatgccagc aagaaatcca
     4321 ccatccctcg tgctcaactg ccccaaacga ttacagcagg agaaatcacg acgaccccgg
     4381 agggccggcc ctttacggtt gtctccttgg ccgaagattc agccgtgatc gacttcaatc
     4441 atcctctcgc ggggaaacag atcgtgttgg acgtcgtggt gctcaaagta aaaccacgaa
     4501 cataggccaa ttttgtgaac gggcagaacc gtgacaggtg gttctgcccg gacgcctctc
     4561 catagcgctc gccttcccgt cattgtctac agttggtaca actaattcac ccggacgagc
     4621 agagccttcg catgtatggg agggctgtat taggatggaa gatattgtcg gagagacttc
     4681 atgcgccgct cgctcacgca tccatcgatc ttggtcatgg caattattgc cgttcaatgt
     4741 tggctgacgc ctctggtgca cagtcagggc agaagtgcgc aagtcactgt cacgccgact
     4801 gccgataagg tcatcgcctg gtcggccgcc ggtggtggtg tcgaagaatc gctgcaaccc
     4861 ggcgagtccg tcgtggcctc cggagcgcgc ggacacacgg gattcgtcca gacctcgacc
     4921 aggttgttag gattttctgc cggccttcgg cagtggcggg aggtgtcgct gggagtcgaa
     4981 gagcggatcg ggcggcatca actgctaccg tttctgattc tcgcgcatag caatcaacag
     5041 gtgtatggat ttcaagaaac gcgcggacac tggacgacgc tgtcgctggg gccaagcgag
     5101 aaggtcgtcc agctgcgagg tcacgggcat gtcgccgtcg ccatcaccaa cgaacgcgcg
     5161 ctgggatttt cgacctacac tggcggcttc ttcaatatcg cctggacgcc tgaagagcga
     5221 gtgctgtccg tcgatgggag tcagaacggc atggtcgtcc gcacgtcatc ccgcaccgtg
     5281 atttttaagt cgcaatccac gggatggacc gaggtgcgat agcgactcgc cacccacccc
     5341 gatcacaact tacatacggc cttcgtccag ttcgatcgca tcgacccagg tggtcgcggg
     5401 aggcagcgcc gtcaccggta cgccccgctt cgtggcgatg tcctgtagac gaccctgata
     5461 ccgctgcttc gccgcctcat ccttcgggcc tccggtgaga atgcccttcg accactcggc
     5521 aatctcttga tcggaatgct tacggccgtt ggttttcacc cagttcaaca gcttctcatc
     5581 gctctcaccg gctaatacct gccgctcgaa ggctttcggg tcgatctgca ggaaatcgat
     5641 caggtactta tcgaagccga ccgtgatgta gttgtagtcc tgaatcttgc ctgcatgccg
     5701 caggcgaacc ttgtcgataa accggcccaa gtgggcgatg cctccgagga gcacctttgg
     5761 actgcgggga taggtttgct gagtcatagt tacttcattc cttttttatt gagaacacca
     5821 tcaaatacaa tcttcccgcg aaggcgacga atgcttctat gccgatgcac tttcacaagc
     5881 aaacgaagcc cttcttcaac aaccgtcttc ttaggtaaaa gtccggtcgc acacatcgcc
     5941 tgccgcatga ggctatcatc aatcacaata gtagtgcgca tgatgccccc gataatgcca
     6001 ggtgttccaa gaacgaagta ctctgcacac ccagttagaa acaattggaa agaaagtcgc
     6061 cttggtggca ctgaaaagcc aaggtttcag ttcatctagc agagtgggca ctgaatccgt
     6121 catggtccaa gatagtagga gatcaggctt ggtgcctctg gttcggcggc catcctcctt
     6181 tgcgaaaagc ctgtgagctc gacttctggc tcaacaagat cggaatcaat tagtaggaac
     6241 tgagtgcctg cgccgtttcc gtcggccgcg aaccggtaaa tttctcgata cagagattgc
     6301 acgagttcgg ggttctcctc atcgctgatg ttcttcgttg gactatcaat gatcaggatg
     6361 tttggaacag gcattccgcg ctcgcgggcg acttcgtgaa cggccaatgc gtagcagacg
     6421 ttgaacagcg tcttcttccc cccgctcccc gtgtcccaga agccccactc ctgtctggaa
     6481 tggacaacga tgggctccca gttgcgcggg tccagcacca catcgtcttc atctgagaca
     6541 ccggggaaac caacctcgag catgatcgct ttgaatctct ctgcaattgc acgagcgtta
     6601 ctgtcggcgg agcgaagtcg tccccgctct tcatcgagcg tcgaacgaag ccgatcgatg
     6661 cgtccctgtg atgctccggc ctgttcctcc agctcactga tggcccgtgg catctcctgg
     6721 agctgcacga gcgaccgcag gcgctcctca agcgtcgcga tctcgcggtc actcacacga
     6781 atactctcaa caaatgccga gtcgtagcgg cgcagttctt cctggagttg acgatctagc
     6841 tgcctctttg tgctttcggc tttctggagc cctcgctcgg accgctccag cgcttgccgc
     6901 ctccggatca ttgagtctgc aatctgatca atacgatcat tgagttctcg ccgaactgac
     6961 tcagcgtcga cgcttggacg atcgtttatg gctgtcgagg gtgtgccaca aaggcgacac
     7021 tgctcgacag cgggtggacg ccctgatacg tcagagccgc attggggaca gtaggaataa
     7081 tcgatgccgt cgaggatgcg gcccgcttgc tcagcgcgcc ccgccttcac cttcgcagta
     7141 atcagctcag ctcgaagtgc ttcttgctca gcgagcgtgc tttgagatgc gctgatggct
     7201 tcacgtaaat cggagacagt tcggctcagc tcgcgcagtc tgctccttag cttgtcggtt
     7261 ggatgaatag tggtcacacg gtctgcttca agcgtctgct tccgttcctc ggcagcttgc
     7321 atttcgcgct cagttgccgc gatcttggct gtgagttcca actcagagcc aagatcgaac
     7381 cgcgccatga accgacggat ctgtgcgaca gactcccggg cggcgtgctg ctccgtgact
     7441 gttcggtaca gatccgcttg cagttgactc aatcgctctg agtgcaaacc ggtgaagtac
     7501 cgcatcgcgt cctggctttt tcggcctcgg aatggatcct ccaagcggaa aaaggacgag
     7561 tccaaatggg tctggtccaa gtagcagtac caccagatgt ctcggaagct gagcctgacc
     7621 atcggagagt ctggatctcg actccgctta agaaccttga tgggttctac gtcgcagagg
     7681 tggaacatca gatcgctgag gctgtagacc tccgcatcca acagcggcgc ttcgcccgcg
     7741 gacagtggcg cttcgactga accgaagtcg tcttctccac tagtccatgt gacgcgaacc
     7801 gcctgcgtgt cttcagcccc gcgctcaaat gtgcactcat actcgccgag tttcgcccca
     7861 aggatgaccg agacgaattc ctgttgaatt gccggcgttc gctccagatc accgccgaag
     7921 cagtagtcaa cgagacgtgc gacggtcgat ttgcctgtac caacgggacc gtacaggaag
     7981 gtgacggtct ctgagaaatg gatcttttcg gtcgtctgcc gagttctgat tacgagatga
     8041 gtcagccgga tattcatgct gggattctct cattcgaccg gagcgacacg acttcaggga
     8101 aagtctcata gataaattgc attagcgtcg ttgcttgcac gtcaaaatgt cgcttcaaca
     8161 gggaagatcg cctcgcgtaa tcttgaaatg ccgggctcgc gctcagctcg aacgccagtg
     8221 cccgcccacg ctctgtgagg gaaatcacga ccttgcgtcc ctccatgctc accgtgacga
     8281 gccccttgcc gacaaggatg ttgaggaact cgcgataccg atggtcccaa gggccaaatc
     8341 gatagcgcac catctgggac tcaacactca gccgctcatg atcctcaagc tgcacatcgc
     8401 gcgtggagcg acccttggcg gccagggcac gctctagcat gacggggtaa cgaaggagga
     8461 agtcgagttt ggcgagcttt gtgagcccct ccactgcgtc tccctgccct gtgcctgaga
     8521 aggccgagat cagcacaagg agacgagcag catgcaggct cggttcagca tcgagcgcag
     8581 cgatcatcgc aaccagatcc atcactgcga ctcccatggc ctggcgtggc tccagacgac
     8641 cttgcactga gcggtcagtg agaaggcgat tccttcaagg tggtcatccg tggagtcgaa
     8701 cagttgatca ccggacctgc gccgctcgcg aaaccggcga cgcaggtcct ctctcattgc
     8761 gggcccgaag ccgtcggcct tggtctgaga ggcgtcgaac gcccgcgaag cgtcactcag
     8821 cacagccgaa cggacgtgat cgtagcgctg gagtcccttt tcgcgtccgt acttcttcgt
     8881 ccatctgatt ccgagataat cggccttatc ccgcaaatcc tccgcagagt tcctcgacac
     8941 cgcggagaat ccgcccgcat cgagcttctt gtgtagcagt tcggtgctcc cctctcctgg
     9001 ctcaacacag agaatgggat caccagccaa ggttgcagta aagttgcggc catgctccaa
     9061 gatgctttga acacgagcaa gcgtcatgcg tttcccatct atccgtgccg ccatagcagc
     9121 atcatcatct aaggccgcaa tcatgtatgc gggcagaagt tgctcgtgat ccagcgaaga
     9181 ggcccggccg cattcgtcaa tcagcgcgtg cgccgctcgc ttcaccgcat cgtgcgagca
     9241 gtcggccgct gaagcccagc attgggcgat ggcatcgatt agccgcatga ctgcgtcgcg
     9301 gagtttgggc aagtccgcct ttgccacggc cttcgacaac gcggcaaatg caaccgtctc
     9361 aggcacatcg gcatcccgag cgactcgcct gacccatggg gcaacgattg atgggaggtt
     9421 tgcgattgac ttggcatcct tgattgactg caggatgtgg ggaaggtctt gtccgtttcc
     9481 agccgagtag agcggatggt tcgtcaagaa gcagaagcga cggaacttcc cgggatactt
     9541 agattctagc ttggcaaacc tgacgaatgc cgcgagaacc gcttcgtcct tggccctcca
     9601 cggcggttga tcgtcgcggc gtgtcttgac ctggtgccca gtgaaccagc cgtctcggtg
     9661 cttgagcagg acatcctcat ggtgttcaca gaatatctct tcaacgtcct cagtgtcgtc
     9721 gagaagcatg cagcacacga ccgcagccca cgtccactga tagcgatagc gattggcggt
     9781 ctcatcgccc gggtcgccgc ccgagactgt gttctcaggg ctagggattg gagcagctgc
     9841 catctgtcgt cacctcgcaa tcggtcagca atcgtgttac cccatagact acttttgcag
     9901 atacttctcc acgcccgcag ccatctcgcg cctttcggcg atggcccaga cgattagcga
     9961 gaccgggagg atgccgcgcc ccagtcgtct tgggtaatca gtgctctggt cttcaatgca
    10021 ccacttgcat aggctagatg aagcgtctct tccagccatg caagccgttg agcgggcata
    10081 gcggagagtg ccgccacgag cagattttgg tcggcagcat tccaaccttc cggttgcgtc
    10141 acattcgaca aatcagacaa cctactgtcc atagcgagcg cctttactca ccgtcgattt
    10201 gaaatatggg ttcgaagacc gacacggcgc tattctagcg gatcagcaga caattagcag
    10261 ccgatctgac aatggcatgg aatgaaagcg ggacagagct ctggcgggga taccgacgaa
    10321 ggatttttat gcagcccagg ccgctcaaaa agcctatcca ggtaggccgc agcgagcgag
    10381 aatccgaggc gtctcgttgc ggtacgttga gaaattgagg gaagcgagac gacgctggga
    10441 ggttttttca gcgacctgtt ttcgcagact tgttggcttg cagatactta tccacgcctg
    10501 ctgccatctt gcgcccttcg gcgatggccc agacgatgag ggaagcgccg cgtttcgtgt
    10561 cgccgccggc aaagacaccg tcgaggttgg tcatgaagtt gtcgtccacg gtgacacagc
    10621 cacgcgcatc gtaggtgacg ccgagactgt cgaggaagcc gtttctgacc gggccggtga
    10681 agcccatggc gagcaacacg aggtccgcat ccaattcaaa gtcggtgttc ggcatcggca
    10741 cgaacttgcc gccctcgaac ttgacgcgat tggcatgcag cttggtgaca tggccgttat
    10801 ggccggtaaa cttggtggtg gagacgctcc actggcgatc gcaaccttct tcgtgcgcat
    10861 gcgacgtgcg aagttgcatg ggccagagcg gccaaggcgt ggaactcgcg cgcgtgggag
    10921 gcggttcggg caatacttca aactggtgcg cctcgctgca gccctgacga tgcgcggttc
    10981 ccagacaatc ggacccggta tcgccgccgc cgatgatgat gacccgcttc cccttggcgg
    11041 tgatcggttc ctcggacacg gaaatgcctg ccgttcgctt attttgttgg gtcaggtact
    11101 ccatggcgaa atggataccc ttgagttccc gaccgggaac cggcaggtca cgcgccatct
    11161 cggcgcccat ggtcagcccg actgcgtcga actgttggcg caactgctca ccggtgatat
    11221 ctttccccac ggtgacgccg gtcttgaact ccaccccttc tgccttcatc tgctccagcc
    11281 gccggtcgat cacccacttt tccattttga aatcgggaat cccgtagcgc aacaacccgc
    11341 cgatgcgatc ggacttctcg aacaccgtca ccgtgtgtcc cgcccgcgcc agctgttgcg
    11401 ccgccgccaa accggccggt cctgaaccga tgatcgcgat ggtcttgccg gtttttctga
    11461 ccggcatggc cggttcgacc aggccttcgt tgaagccgcg gtcgatgatg ttccattcca
    11521 gcacacggat cgacacggga tcgctattga tccccaatac gcaggctcct tcgcaggggg
    11581 cggggcagag ccgaccggtg aactcgggaa aattattcgt ggtgtgcagg gctttgagcg
    11641 catctttcca gcgcccgcgg tagacgagat cgttccactc cgggatcagg ttcacgaccg
    11701 gacaaccggt attgccctga caaaagggca caccgcaatc catgcaacgg gcgccctgga
    11761 ccttgagctt gtcctcagcg atcggctcgt acatttcctt ccaatcgagc acacgcaact
    11821 cgaccggttt ccgcttcggt ccctcacgcg cgtatttcag gaagcccttt ggatcaccca
    11881 tcgcttaatc gtctttcgtc gttcgtatct cgttcaagac ttttcaatca cgcacgcgct
    11941 tcacgaacac cgcatcacgt ttcacggtta cttggctact ttagccgccg ccgctttccg
    12001 ttcggccagc acacgcttgt agtcaatcgg catcaccttc acgaacttcg gcaggatcac
    12061 ttcccagcca tccaggatac gcttagcgtt ccggctgccg gtatacatga agtgggaggt
    12121 gatcatgtcg tgcaacaact tcttatcatc gtcgctcgtg acctgctcca actccaccat
    12181 gcccaggttg cagcgcgact ggaatttatc caactcgttc agcacgaacg cgacgccacc
    12241 cgacataccc gcggcgaaat tcctgccggt gcggcccaac accgccacaa ccccgccggt
    12301 catgtattcg caaccatggt cgccggtccc ttcgacgacg gcgcgcacgc cgctgttccg
    12361 caccgcgaac cgttccccgg ccatgccgta gaagtaggct tcgccctgcg ttccaccgta
    12421 cagggaggta ttgccgacca ggatggtttc ttccggcgta taaatcgcgt tcttcggcgg
    12481 gaagacgatg atcttgccgc ccgagaggcc cttgccgatg taatcgttcg actcaccctc
    12541 caacacgagc gtgatgcctc gcgagagaaa cgctccgaac gattggccgg ctgatccatt
    12601 aaacttgatc gtaatcgtat cggcaggcaa cccttccaag ccgtatttct ttgcgatctg
    12661 gctggagagc atcgtcccga cggtgcgatt caaattcctg atcggcagtt cgagcgtaac
    12721 cttttcgccg cgatcgatcg ccggtcggca ttgctcaatg agcttccgat ccaaaatgtc
    12781 cgcaatcccg tgatcctgct tctgcacgca gtagcgagac acctccggac cgacttccgg
    12841 catcttgagc agtggcgcca gatccaggcc cttggccttc caatgttcga cggccttgtg
    12901 aatcttgagc ttgtccacgc ggccgaccat ctcgttgatg gtccggaatc ccagcttggc
    12961 catgatctgc cgcaactctt cggcgatgaa gaagaagaaa ttgacgacat gttccggctg
    13021 gccggtgaat tttttgcgca acaccgggtc ctgcgtggca atgccgactg ggcaggtatt
    13081 gaggtggcac ttccgcatca tgatgcagcc ttcgatgatg agcggggccg tcgcaaaccc
    13141 atactcttcg gcgcccagga gcgcggcaat ggccacatcg cgaccggtct tcatctggcc
    13201 gtcggtttcg acccggatcc ggccgcgcag gtcgttgagg acgagcgtct gatgcgtttc
    13261 cgccaatccc agttcccagg gcacaccggc atacttgatg gacgagagcg gagaagcccc
    13321 ggttccgccg gagtcgccgc tgatcaggac cttgtccgca tgggccttcg ccacaccggc
    13381 ggccaccgtg cccactccga cttcagagac cagcttgacc gagacggctg catcggagtt
    13441 cgcattcttc aagtcaaaga tcagctgcgc caggtcttcg atcgaataaa tgtcatggtg
    13501 cggcggcggg gaaatcagct gcacgcccgg tgtcgaatac cgcagtcgcg caatgttctc
    13561 atccacctta tgaccgggaa gctgtccgcc ttcacccggc ttggccccct gggccatctt
    13621 gatctggagt tccttggcat tcacgaggta gtggctcgtg actccgaacc gtgccgacgc
    13681 cacctgtttg atatagctgt ttctggaatc gccgttcggc agcggcgcaa accgctcggg
    13741 atcttcgccg ccttcgccgg tgttgctctt cgcgcccagc cggttcatcg cgatcgccag
    13801 cgtctcgtgg gcctctttgc tgatcgaccc gaacgacatc gcgccggtcg tgaagcgctt
    13861 cacgatgtcc ttcgccggct ccacctcgtc gaggggaatc gcctcaggct ggaacttgaa
    13921 ctccagcaga ccgcgcagat tcgaccggcg cctgctctca tcgttcacca gctgtgaaaa
    13981 ctcggcgaac gtcttgggat cgttgttcct ggtcgcgtgc tgcagcttat agatggtgtc
    14041 gggattccag ttatggtgct cgccctggat gcgatagtga atctcgccgc cgaaatccaa
    14101 ctgccggatc ggggccggct cgtaggccac gcggtgacgg cgcaatgtct cttcaccgat
    14161 ttcgcggatg ctgatgcctt cgatccgcga cggcgtgccg gtgaaatagc ggtcgatcaa
    14221 ttcgtgattg agtccgatcg cttcgaaaat ctgcgcgccg caataggatt ggaccgtgga
    14281 gatgcccatc ttcgagaaaa tcttgagcaa gcctttgtta atagctttga tgaacttgcc
    14341 ttccgcggtc ggtgcgtcga gcccttcggg gaaatagccg tcacgctcca agtccaccag
    14401 cgactcgaac accaggtagg gattcaccgt cccggctccg aaaccgatca gacaagcgaa
    14461 gtgatggaca tcgcgcggct cgccggtttc caccgtcagg ccgacttcgg ttctcgtgca
    14521 ttcccgcacc aggtgatggt gcaccgccgc gacgccgagc agacttggaa tcggggcgta
    14581 gtccgcattg accccgcggt cgctgaggat gaggaacttg tatccttcac gaatcgcctg
    14641 cgaggcctgg cggcagagat cgtccaccgc cgcgcccaac ccttccgggc cttcggcgac
    14701 gcggaacaac atcttcagcg tcttgctctt gaaattgggg tcgttaatct cgcggatctt
    14761 ctgcagatcg gcgttcgtca ggatcggctg cttcacccgg atgcgccggc aggactccgg
    14821 atgttcatcc atcagattag gcttgggacc gatgctggtc gtgagcgaca tcacgagttc
    14881 ttcgcggatc ggatcgatcg gcggattcgt gacctgcgca aagagttgct tgaagtattt
    14941 gaacagcagt tgcggtcgat ccgacaacac cgccagcggc gtgtccgtgc ccatcgacga
    15001 gatcgcttcc tgcccttcca ccaccatagg ggtgatgacc atcttcagtt cttcgacggt
    15061 gtagccgaat gcctgctggc gctgccggat cgtgggatga tcaggctgcg gcacgttgat
    15121 cgggtccggt aactcatcga gcgaaatccg atattgagcc acccaggagc gatagggctt
    15181 gcgacgcaca atatcggctt tgacttcttc gtcgtcgatg atgcggcctt gcacggtgtc
    15241 cacgaggaac atccgtcccg gcatcaaacg gcccttctgg cgaatccgct gcgggtccat
    15301 cggaagcacg cccgcctcag acgccagcac caccagaccg tcggtggtca cctgatagcg
    15361 gcacggacgg agtccgttcc gatccagcgt ggcgccgatc agcttgccgt cggtaaaaca
    15421 gacggccgcc ggtccgtccc agggttcctg catggccgcg tggtactcgt agaatccacg
    15481 gcgatccagg tccatctgcg gattggcgac ccagggttcg ggaatcaaca tcatcatcgc
    15541 gtgcggcaac gaccgcccgc cgagcaccag gaattcgagc gcgttgtcca aacaggccga
    15601 atcgctctgg ttctcgtaca cgatgggaaa cagtttctgc atgtcctcgc cgaacagttc
    15661 ggtattcagc cgcccttgcc gtgcgcgcat ccaattcaca ttgcccttca acgtgttgat
    15721 ttcgccgttg tggcaaatgt agcgataggg atgggccagc ggccaggtcg ggaaggtatt
    15781 cgtgctgaat cgcgagtgca cgagggccag gccgctcgtc acgctgctgt cggtcaggtc
    15841 ttgatagtac tggggaatct gatgcggaag taacagcccc ttatacacga tggtgctgct
    15901 ggacaggctc gggatataga agtactcacg cccttcgatg gcggattccc gcaccgcccg
    15961 ctctgcgcac ttccggatca catacaaacg gcgctcgaat tcgtcgtccg tgaaaattcc
    16021 ccgggcgatg aacacctgcc gcatgaatgg ttcggtcgtg cgagccagct caccgattgc
    16081 gtcgctcttg accggcacat cgcgccagcc gagcaacttg gcgttggcgt ctttgatgac
    16141 gcggttaaac accgtctcgc actgcacacg ggcatcggca tcgggcggca ggaacaccat
    16201 acccacgccg tattctcccg cgccgggcag cttgatcccg cagtcttttg ccgcgcgctt
    16261 aaaaaactca tgcggcactt gaaggagaat gcctgcgccg tcgccggtgc agggatcgca
    16321 cccttgcgcc cctcgatgag tcagactctc caatacctgc aggccctgct gcacgatctg
    16381 gtgcgagcgc tggcccttga tatcgacaac aaacccgacc ccgcacgcat ctttttcgtg
    16441 ctgaggatca tataaccctt gtttgggagg aagccctggt acattcatga cacgtgttct
    16501 cgtcaagctg gaaggcaatt acctggggaa cagactcccc tcacggggac gttgaagctg
    16561 tcgaacgacc accggtgact gctatcattc attcggggaa ttcagcgagg agtcacctta
    16621 ctggatgggg ctggaaactg tcaagagaac gaccacgcga tcgacgcgag aaacgcatcg
    16681 gcaggagctt gacttagtgg actttcctgc cctactatcc gctccatgtc gaacggaccg
    16741 accaccctca cgctccacag catggccgat gtgtgggacg tgtatcgcga agaactcgag
    16801 ggggtcgagg aacagatccg gaaaaatctc gactccagcg tcgctctcgt caacaccgtc
    16861 gccgcccaca tcctgaacag cggcggcaaa cgcgtccgtc ccctcttcgt gctcctcagc
    16921 gcccatctct gcggatatgc cggccaggac catcaagcac tcgggagcct ggttgaattc
    16981 atccataccg ccaccctgct gcacgacgac gtcgtcgatg acgccgacct gcgccggggt
    17041 cgccggaccg cgagcaaagt ctggggcaac caaatcagta ttctcgtggg cgactatctc
    17101 tattcgcgcg cgatctgtca gatcgtcgac ttccgcaatc aagggatcaa cgaggcactc
    17161 tcggaagcct gccgcaagat ggccgaaggc gaagtgctgc aactgtatta caacggcaat
    17221 ccgctgatgc cggagtcgga atatctccgc atcatcgaac ataagaccgc cggattgatt
    17281 gcggcgtctt gcaaaatcgg cgcgatcgtc ggtggcgcca cggaagagtt acaagaggcg
    17341 ctgttccggt tcgggcagcg gctgggcatt gcattccaac tggcggacga tacgctggac
    17401 tataccgcca acggtgaaca cctgggcaaa acactcgggc aggacctgcg gcaagggaaa
    17461 gcgaccctgc cgctactgca cctcctgcag cactgctcgg agccggatcg gcaactgatc
    17521 aaggatcgca tggagactcg caccctgacg gacgaggaac tccgccgcat cgtcggcttg
    17581 atgcaggagt atgggtccat cgcctacgcc atggagcggg cacgcgcgtt cgtcgccgcc
    17641 gccaaacgcg atctcgacct gttccacgac aacaccgcca agcgcgccct ggccatcgcc
    17701 gccgattaca tggtgactcg cgaccgctga gttgccgccg cccggcttca caacgcccac
    17761 gctctgctga cagcgcgtac tcgttcaacg acacacccgg ctcaccgccg ggcgaacagg
    17821 accacaccgc acccacgaga gaaaaggata ggaacccgca tggcacacgt cattcccttt
    17881 cacggtacac tctataatcc tgcgaccgtc ggcgacgtcc gccaggtggt ggcaccccca
    17941 tacgatatta tcgacgcgac gttgcagaag accttgcacg atcgccatcc gaacaacgtc
    18001 attcgcctgg aactcggcta tgaacagccg ggcgatacca ccggcgacaa caagtacctc
    18061 cgcgccgcgg gtgccctcaa ggagtggctc aagtccggcg ccttgcgtcg ggacgacaag
    18121 ccggccatct actaccacac catcgaatac cagccgccct attcggcgcc gggcaccccc
    18181 accaaagtct tcaaaggttt tctctcaacc gtggagctgg aagagttcgg gtccggcaag
    18241 atctacccgc acgagaatac acgcgccgca gccaaaaccg accggttcaa tctgctcgaa
    18301 gcctgccgcg caaacttcag tgcgatcatt tcgctctatt ctgatccgca gaacgacgtg
    18361 ctcacgttga tcgagcagtc catcgcctcc gacaaaccac gcatcgactt tcaggacgat
    18421 gtcggcttcc gccagcggct gtggagcgtg accgatcccg ctgtgctcgc caaggtcgtc
    18481 gagatcatgc acacgaaaca gctcttcatc gccgacggcc atcatcgcta cgaaacggcg
    18541 ctcaactatc gccgcgcgcg acgccagcaa gccggtgccc cctctggccc ccagccttac
    18601 gacaatgtcc tgatgctgtt cgccagcctt gaggacaagg gcctgaccgt gctgccgacg
    18661 caccgtgtgc tcacgacggg cgtcccggcc ccgaaagatt tgctgcgcat gcttgatcct
    18721 gtattcgaag tgacgacgct gccttttcag gccggcaatg aggcgcaggt acggggacaa
    18781 tttatcgaaa ccctgcgcag ccgcggacag tccgtcccga tgttcgggct ggcactgaaa
    18841 aacgatccgc agtactacct gttgaccttg cgagaagcac atcgcccgac ggcctccgcc
    18901 tcaccgcgcg accggctcga tgtgtccctg ctgcagcagc atgtcgtcgc tacgctctgc
    18961 cccacccagc aggaacagga agcgatgctg tattcgaaag acgatcacga agctctgaat
    19021 tgggtgcgcc aggggaccgg caccgctgca ttgctgctga acccgaccaa agtcgccgaa
    19081 gtgaaggccg tcgcctccgc aggcgagcgg atgccgcaca agtcgaccta cttcttccct
    19141 aagccgctca ccggtttggt catgaatgtg atggaaggat gacggcgaac agcgcacagc
    19201 tgatcgtctc tgacgggtcg cgataccgat gaaggccaag gttctcatag tcgacgacga
    19261 ccaggacatc gtcacgatgt tgcaggatcg tctcgacgcg ggcggctata aaacgctgac
    19321 ggcctccgac ggcctgcgcg gcatcgagct catcgagcag gaatccccga acctggtgct
    19381 gctggacctc tacctgcccc gtctcaaggg catggacgtg ctcaagcgca tggcccaaaa
    19441 caagcagtcc gaggacatcc ccgtcattgt catgaccgcc gccggcacga tccctgacgc
    19501 cgtggaagcc atgcgccacg gggcctacga cttcctgacg aagccgctcg aaaaagacca
    19561 tctcctgatc gtgattcaaa aagcgctgga gcgggactcc ctgaagcggc aggtggccgc
    19621 ccttcaatcg gacgtgcaga accggtatgc caccatcgtc ggcgacagcc cgaagatcca
    19681 gaccatcatc gaatccgcgc gtcgcgccgc caactcggac gccagcattc tcctcctcgg
    19741 ggaaagcggc accggcaagg aactcttcgc gcgctccatc caccaatgga gtccgcgcca
    19801 gagcatgccc atggtcgtca tcaattgtgt ggcgctcacg gaaacgctgc tcgaaaacga
    19861 actgttcggc cacgagcgcg gcgccttcac cggcgcagat cggcttcaaa agggcaagct
    19921 cgaaatggcg gaggggggaa cggtgtttct cgacgaaatc ggggacatgc cgttgccgct
    19981 gcaggctaag ctcctgcgcg tgctgcaaga caaggaattt caacgggtcg gcggcacccg
    20041 ctcggtctcc gtcaacattc gcttcgtcgc cgcgaccaac aaggatctca agcaggccgt
    20101 caaggcgggg cagttccggg aagatctctt tttccggctc aacgtcgtca gctttacgct
    20161 cccgccgtta cgggaacgga gcgaagatat cgtgcggctg gccgagttct tcctcaagcg
    20221 ccatgcctat gaagccaaac gtcccggagt ctcgctaagc ccggcggcgc gcgccgcgct
    20281 gacccactat ccctggcccg gcaacatccg cgaactcgat aacgtcctct cacgcgccgt
    20341 ggtgctcagc cccagcgacg tcatcgaacc tgaatttctg gccctgtctc ccgacgacgc
    20401 agccgggaaa ggaggctcag aacacgggct tccctacctg aacctcacgt accatgagtc
    20461 catggaagcc cacagtgcct acatcattga gcgggcattg gaaaaagcgg cggggaacca
    20521 gacgaaagcg gcggagtttc tagacctgca gcgaacctat cttgcgcgac tgattaagca
    20581 gcggaaatcc actcccgacg agtaaccagg caggatggtg aaaacgaccg tcagcttcgt
    20641 tctcgcaaga ctctgccgcc tcaccgtctc ggcggcgttc acaaacgtga cgcgctttat
    20701 tcagcgcgtc gtgaacctcc gaagctcaac gtaccgcaag ggtacgcctc gcctcctcgc
    20761 attgctgcgg ccttgctgaa cggccgtttt gaccatcctg caagagcctg ctcagcctga
    20821 atccgaccgg ctgccgacca agtcaagtga ccaccaacgc gtagttctcc agccaattag
    20881 gcctggctta cgactttcgt gctgacaatc tgagcaaccc ggctgccgca tcctgctctg
    20941 cttgcgcata ccgctctggt gtaccgacat cgctccagta gccactgaaa tcgtacccga
    21001 ggattcgttc cccttcttga atgccgcgca cataggcgtc gatgatcgag gattctttcc
    21061 ccaccggtac cgcacgcaac aaacgcggat gcagaatatg gatgccggcg aacatgcgac
    21121 cggtcgtcgg tatcgtctcc gtacggccgc gcccggtgat ccgcaccact tcaccccggt
    21181 ccgtcacctc gaccaacccc caccgcgcgg cctcggggtc ctgccgcagc accagggtcg
    21241 ccgcagcctg ctgcgccaca tgaaacgcga tcaatgcgcc gagatccagt tcaaacagcg
    21301 tgtcgccgtt cagaatcaac acgggctcgc cgtgaaaatg tggctccgcc tgcttgatcc
    21361 cgccaccggt accgagaatc acgggctcat tcgaatagat gagtcgcatc ccgaaggctg
    21421 agccgtcgcc cagcgcctgc gggatcatcg cacccagatg atgcaggttg atcaccacct
    21481 cgcgaatacc gtgccgtttc aagaggagca gattccagac gatcatcggc gtgccggcaa
    21541 ccgggaggag cggcttcggc gtcacgtcgg tgaggggcct caacctggtt ccaaggcccg
    21601 cggcgaggat catggcgttc atgtgcagca cacgtgaaag gtgaaccgtg aaagatgaag
    21661 cgtgaaaggt gaaccgtgaa aagtaaaacg tgaaagagac atgatcgtgc agaattcttc
    21721 tctcacggcc cgtttcacgt ttgtcgtttc tcgttttact gcttattgca gttcagaaac
    21781 gtagggcgcc aagtgctgac gtaatgtggc cagctcgggg tatttggata aattccgctt
    21841 cacgtagcct agaacgcgag ggatgtcggc gagaaatttc gggttgtgtt tgacccggtc
    21901 gatatagaca aaccggccgg cggccttgag gttgcgttga atactggtga agtcgaacag
    21961 gcggcggaac gccgcccgat ccgcccagcc ctgcccctgg gcggcaaggc catcgaggaa
    22021 atgatcgacc agcccatcca ccaccgcatc atccaactcg atgtaggcat ctttcagcaa
    22081 cgaggccaga tcgtaggttg ccggacccat gagggcatcc tgaaaatcga tgaccccgat
    22141 gcgcgatccg tcgaccatga gattgcgtga atggtaatcg cgatggacga agacctgcgg
    22201 ctgcccggcc agcaattcgg cgatacgttg aaactcggcg cggaccgcct tgtcgtcggc
    22261 cggtttcatg ggctgcccga ggcgggcgac aatcccgtat tcgaggaagt ggtcgaactc
    22321 ccacatcaac agcggcacat cgaatccccg gtgaaacgcg atacagccgg gggcgggcgg
    22381 ttgcgtcccc ttcacttgaa gcagcaccaa ttgatcgatc gcctgacgat agagcgaaac
    22441 caaccgggca cgatccgcat cgcggcaggc ttctgccaac gtcagatccc cgaaatcctc
    22501 caagtacagg aggccggcct cccgatcgta atagtgcaac tgcgggacgg tgacaccggt
    22561 ccgctgcaga tgcgcgagca cgttggtaaa gggcagttct gcaatttgca cggacgcgcc
    22621 gctgacggct tcttcagact ttttgaaacc ctccggatcg gccaactgca tgaggatcaa
    22681 tgccgatggc gtccccttca aggcgatccg gaagtatcgg cgattcgatg cgtcaccggc
    22741 gagcggggtc aaccccgtca catcgccgcg aaacgggagc ttggtcgcaa ctgtcttggc
    22801 gattcgtgca agatccggcg gggcaagcgg tgccgcggga gcggcaggat ttgtcgttgt
    22861 catacgatcg gcattatagc caaaccttgc tgaactgcca gcagaaagtc acctgctgca
    22921 tgcccctcgt ctgccgattg gttgccagcc ctcaccacga gccggtatcc tatctgctct
    22981 accccaagga gacgcacatg cactacgttc atctcgggcg ctcaggcgtg cgggtcagcc
    23041 gcctctgtct cggcactatg aacttcgggc cgcagacctc tgaaaccgac agctttgcga
    23101 tcatggacca agcgctcgaa cggggcatca actttttcga cagcgccaac gtctatggct
    23161 ggaaagtcgg cgaaggggtg acggagcaga ttgtcggccg ttggctttcc caaggcagcg
    23221 ggcgacgaga gaaaattgtg ctggccacga aggtctatgg ccgcatgggc gactggccca
    23281 accagtcgcg cctgtctgcc ttgcatatca aacgcgcgtg cgaagagagt ctccggcgtc
    23341 tgcagacaga ccatctcgac ctctaccaga tgcatcacat cgatcgtgaa tgcccctggg
    23401 aggaaatctg gcaggcgatg gaacaactct gccgcgaagg gaaggtgctg tacgtcggca
    23461 gcagcaattt cgccggctgg cacatcgcgc aggcgcaaga gatggcgaag tcacgccact
    23521 ttctcgggct catctcggaa caaagtctct acaatctcct ggagcgaacc gtcgaactgg
    23581 aggtcatccc agcgtgccag gcctatggga tcggcatcat tccctggagt cccctggcgc
    23641 gcggcctgct tggaggagcg ctgagcacgc acacaaccgg gcggcggaca gacgaagacg
    23701 tccggaaata tatcgagacc catcgcacaa aactggaagc ctatgaacag ttctgcaaga
    23761 ccgtgaatgc caccccggcc gccgtcgccc tcgcctggct gcttcatcaa ccggccgtga
    23821 ccgctcccat catcggccca cgcaccatgg aacagctgac atccgcactc gcggcgttgg
    23881 agttgatgct gacgccggag cacctcaaga cattggacac aatattcccc ggcccgggcg
    23941 gcgccgcccc agaggcctac gcctggtaga agttaacaac gaaaggcaag ctcagcggac
    24001 catctgcatc gtcatcacgc agggaaagga gcgaggatcg gagaaacacc gcggcaagaa
    24061 tcgatgggac aggaccgggc gaaacgcgtc gaggctacag agatgactga acagcgcgcc
    24121 gttccatgag acgccaggtc caggccccga gcgcggcacc cacggaatcg gccaccagat
    24181 ccagcgggtc cccctgacga agcggcacga acaattgatg gatctcatca ctcaggccat
    24241 agagcgcaca accggccacc gccacgacga cggcatgccg ggccacccag gcacccgctc
    24301 cgtgccggca ggcccgatag gctaaggcgc caaacactcc atattcgcag agatgcaaga
    24361 tcttgtcgga gatcttttcc attagcgacg aaacggactc cggcggattg gatacagaag
    24421 acccgaagaa aatgagtccg gcatacacac agaccgggcc ccaataccgc aggaccgaaa
    24481 aaggggatac cagcggcgct gtacggatct cgcgcgaaac catctgattc gattccattc
    24541 tgccaggctc cccgtttcac accaaagtcg atcccgtacc gcatgatcgc tctcacccgg
    24601 gcggaaagaa aacaggcgct cctcaatcct gccctttcat tgcaacactc ctacccctat
    24661 gacatactgc cctggagtct gcaagaaact tcccgatgaa aaccattcaa ctctgcttcc
    24721 tctggcatat gcatcagccg tattacacgg acccgctcac ggggtccgcg agtatgccct
    24781 gggtgcgtct gcacgccacc aaggcctact tcgacatggc cttcctgctg gagcgatttc
    24841 cggaggctcg ctcgaccttt aacttcacgc cgtcgctgct gctgcaactg gaagagttct
    24901 ccaccggccg cgtccgcgat ctcttcctgg aatatgcgca gcgcccggcg gctgagctga
    24961 ccccgactga aaaagccttc ctgatccgtc atttcttttc cgccaactgg gccaccatgg
    25021 tacggccctt tccccgctat caggaactgc tggtgaaacg tggcgtcgac gtccatgggc
    25081 aagacctcga tcgtctggcc agacagtttt cgacgcagga atttctggac ctccaggtct
    25141 ggcacaatct cgcctggttc ggatacggca gcctgcagcg ctttccacgt ctggcggagt
    25201 tgcgcaagaa gaatcgggga tttacggaag aggacaagca ggaagtgctg gcgctgcaac
    25261 agaccgcaat ccggcagatt gtcccgatgt atacggcgct ccaggagcgt gggcagatcg
    25321 aattgaccac cacgccgttt ttccacccga ttcttccgct cgtgatcgac tcggaattca
    25381 ctcgccgcgc cagaccggac ctgccgctgc ccgctcgatt ccatgctccg gccgatgccg
    25441 aagcccaggt gcggcgggca atcgactacc acacgcacac gttcggccgt gcaccggccg
    25501 ggctctggcc ctctgaagga tcggtctgtc ctgagttgtt gcccatcctg ggcaaaaccg
    25561 ggatccgctg gctggcgacg gacgaaggta tcctctaccg atcgcttcag atggccgacc
    25621 agacctggaa tcgtcattat cacctctatc agccctacgc agtcggcacc gcggagcagc
    25681 cgctcaccat ggtgttccgc gaccgggata tttccgatgc cttcggcttc gtctatcaca
    25741 agacgacccc ggagtcggcc gccgacgatg tgctcaggcg aatccgcggc ctcgcgtacg
    25801 acattccgct ggagaacggg ctcctggccg tgattctcga cggcgagaat ccctgggagc
    25861 attaccacga cggcggtgaa cgcttcctct ccctgttgtt tcgcgccttc gaacaggacg
    25921 ggctccatat cggccacggc atccgcgtgc gattgaacac cgtgagccaa gccttggagt
    25981 cggttccgcc ccctcaacgc ctcgatcagt tacattccgg ctcctggatc aaccaggact
    26041 ttaaaatctg gatcggccac caggaagaca atcgcggctg ggacctcctg cagcacacgc
    26101 gggcccgtct cgtggacctc acgccctcgt tggcgcagga caaggcccgc gcagcctggg
    26161 acgaactcta tgccgctgaa ggcagcgact ggttctggtg gtacggcgac gacttcgata
    26221 cggactacaa acaggaattc gaccgcctgt tccgcacgca cctgcgcaac gtctggacct
    26281 atgccggggt gacgccgccc gacatcttga atcagcctct ggtcgaagct cgcacgccgc
    26341 aaggcctgga cctggtgcaa ctcccgctgg cgctcatcac cccgaccctc gacggcatgg
    26401 tctccaattt tttcgaatgg cgcggggccg gcaccatcaa cccgacgccc ccgctgggag
    26461 ccatgtggaa gtccgaagga ctcttcacct ccatcttctt cggattcgac cgcgaacatc
    26521 tctacctccg gctggatctc gatgaacgaa ctcaaacgcg ccaggagcac tgcaccgcag
    26581 acctattcat cggctcgggc atccagcaat ataagttgtc gtttgccctc acggccgaag
    26641 gcgcggacac cttcctgctc tcccgggcgg acgagtccgg cctctatcgc gacaggggct
    26701 cctaccgcac gatctgccgg cgaaaaattc tggagctggg cataccgttc aaagaactgg
    26761 gcatcgagat cgacacagaa ctacggttga ccctgacggt gtcggaacat gggatggaaa
    26821 tcgcgcggta tcctcaccac agcccggcca ccttcaaccg gccgggagac gactttgaag
    26881 ccacgatgtg gcgcgtctga acgacaatgc tcaaggctcc ctgttcaatg aggtcgtcac
    26941 catccgcccc gtcattggac atcgagcatt gcgcagtgac cattgacaac tagaggagtc
    27001 gagatgcctg aactgagaag agacccgatc gtcggccgct gggtcatcat ttcaaccgaa
    27061 cggggcggac gcccccagga cgtgtctatg ccgtcggcgc cgcttccctc tgcgtccatg
    27121 tgtccgtttt gcccgggcca ggaacgtctg acgccgaaag agatcctcgc ctatcgtccc
    27181 catgcctcgg aaccggacag cccgaattgg accgtgcggg tcatccccaa taagtttccc
    27241 gcgctgcatg tcgaaggcga tatgggacgc gaaggcctcg gcctctacga tcgcatgaac
    27301 gggatcggcg cccacgaagt catcatcgaa actcctaatc acaaggaaaa cctcgccgac
    27361 ctgccggcaa agcgggtgga ggatgtgctc tgggcctatc gcgaccggat tctcgacctc
    27421 aagaaggatc ttcgcctgcg gtatattttg atcttcaaga atcagggcgc caccgcgggc
    27481 gccacgttgg agcatagcca ctctcagctc atcgccctgc cggtcgtgcc caccagcgtc
    27541 ctcgaagaaa tcgacggatg ccggcagcat ttccagcaga aggaacgctg catctattgc
    27601 gacatcctgc ggcaggaatc atcggaaggc acccgcgtgg tgctggaaaa cccggaattt
    27661 gtctgcctga ccccctacgc gccgcgcttt ccgtttgaaa tgtggattct ccccaagcgt
    27721 cacgccggat acttcgaaga atgccaacgc gctcagttcg aattcttggc gcctatcctg
    27781 ggcgaagcgt tacgccgaat ggatgccgtg ctggctcgac cggcctacaa cttcatcctg
    27841 cacagctcgc cgctgcacga aaaaaccggg gactattatc attggcacat cgagatcatc
    27901 cccaaactca cccaagtcgc tggcttcgaa tggggcaccg ggttttacat caaccccgtt
    27961 gctccggaag aagcggccaa ggcgctgcga gacgccgaga tctaacggcg ccgacacgtc
    28021 gttttctctc gaccaccgtg cgggcgcacc attcccgcgt gatcataggc tcatccttcc
    28081 ctgaagcaac gatgctgcac gacaggtatt ggataaattt ccgttgctac gtgaattttg
    28141 tcctgtacct ttccttgcca tttacagata cgccaagtat aaaatccacg caatattttc
    28201 agggcattga gaaccgccgg acggccaacg cccacgctca ctcaattcag acattcatcc
    28261 acgtgtaatg ggatggggag gcagcgacca ccaggaaagg taggagggta ccgtgaaagc
    28321 gcacgacctc gaatggaaag acgaaacgtt cagcgaactg gatctccaga atcaaaaatt
    28381 caagcgtctc gcaatcggat ccgccctcct cttcctcagc gtggtcggct tgctgattta
    28441 ccaaatcatc gcgggcatca agtagccaac ggcagatcgt cgcaccctat gcgcgaaggt
    28501 tcgtactgct gcggctccat cggtgcgtgc ccttccttcc atccgaaatc gcctaccgca
    28561 ccagcgcaca tccaggcaac agcatcccga ccggattgca atcccgcaca actcagccgt
    28621 atcattgcgc atgcttgttc atctcagtca cccgcaacgc caggttgaaa tcaaagggcc
    28681 caaacgcacc aaggagttgc tacgcgaact caatctggtg attgaggcgc atctcgtcat
    28741 ccggggcgat gaactcgtca cggaagatga aatgcttgcc gacgccgacc agattgaaat
    28801 ccggccggtc atctccggcg gctgaactgt gcgatcctcc tctcaccatt cacgattgac
    28861 ggaacagtta tgaattgcgg caagtgcaag accaaagccg ttatcagcct tccgcgacac
    28921 aatgccgcct tctgcaaagg ctgcttcacg acctttgtgc acgagcaggt cgcgcgcgcg
    28981 ataaagtcgt tcaagatgtt tacgcccgag gaccgcatcc ttgtggccgt ctcgggcggt
    29041 aaagacagcc tcgccctgtg gcatatcctg ctgaccctcg gctaccgcgc cgacgcgctg
    29101 tatgtgaatc tcggcatcgg cgcgtactcc gaggaatcgc accggaaggt gcgccactat
    29161 gccgacaccg tggccgccgc ccatggcgcg aaactgatcg tgcatgtcgt cgagcaggaa
    29221 gccggcgccg gcattcgtga attggccacc gtccttcatc gcccgacctg ttcgacgtgc
    29281 ggaaccatca agcggtatca gttcaatcgc gctgcggtgg aacaggacta tgacgtgatg
    29341 gccacgggcc ataatctcga tgatgaggcg gcgcgtctct tgggcaatgt attacactgg
    29401 caggaagaat atctggacaa gcaaagcccc accttgccgg cctccgtcga aggattcgcc
    29461 aagaaggtca aaccgctttg ccgtctttcc gaacgcgaga tcgccgccta cgcggtcgtc
    29521 aatcgcatcg attacatcgt cgaagaatgc cccatggcca agggatccaa gatgatcctc
    29581 tataaagagg tcctcaaccg gctggagacg gaatcacccg gcacgaagca acgattttac
    29641 tgggggtttc tcgagaagca gaccaagccg gagcccgcca cggaatccat ggctgagaag
    29701 gatcagcgaa cgctccaccc ctgcacgact tgcggccaac cgaccactgc ggaaatctgt
    29761 acctactgca agatgatggc gaaagccaag accgcgaccc cgcgttaggc tcggcctgaa
    29821 cgcctggtca tagtccggct gacgctcccg tcaatagatg ctcttgctca cttcactcga
    29881 acggaggctc tcattcccgt tcacatcata cgccgtgacg gcaaagtagt aggtcgttcc
    29941 gagtttcagg ttcgaaaact gataggatgt ggccttcccc acgtcaaccg atgccccata
    30001 ctggccggaa gaggttccgg aatagacttt gtatccggct aagtcgcttt cggcattggc
    30061 attccacgcc agagcagccg tcgtgccggt ggtgcttgaa gaagtcgcag ctgagagctt
    30121 aagtgtcacg ggaatcgcca cggtgcttcc cctgctggtc gtgatcgtca ccgttccgga
    30181 ataggttccg gccgccagcc cgctgatatt gacggacacc gtcacctgcg tggaacttcc
    30241 cagcgtgccg gacgtaggag agagactcag ccaaccggca ttgtccttcc cgctccaact
    30301 gacctttcga ctgctgctct tgtagacctt caccgtctta ctggatggat tgggaccacc
    30361 ctgcacggcc tgaaagctca aactggtcgg actggcttgt aaggcatggg cttcggaaag
    30421 catcatcacg cttccgatca gcatcacggc aagggtcacc agcatccgag gtaggacacc
    30481 cacaggcaaa gagaaccact ggcgcagacc tcgtttcata tgacctccca cgtgcgacat
    30541 tagggaatac catcttcgac aatcgcaaag cggctcaccg cgcgtctcgt gacacgaacc
    30601 actgccccag ccagacactc taagagcagt gatcatgcca agctgaagtg cgtgagaaaa
    30661 tcgtgctatc tcgtgaggat ttacgtgtat gtccgccgcg gcttgggcgg ccggtgtatg
    30721 gttgaaactt caggacgggt agagaatgtg cacgcagccc cctcgtctcc ggtgcggaga
    30781 aagaggaagc ggtggtgact cctatcgatg tgttgccgct caaggaactt ggtacattcc
    30841 gtcgaccaca tgttccacga gaagccaact tgccgggaga gacgcttgtg ctcgccgtcg
    30901 cgcgagaaaa aatgatgaga tccgcaagtg ccgagccgtc gacaagacgg tgccggcgca
    30961 aaggttcgag ggtcccgcgt caggacgggc ccgacccgtg ccgtgtctac ggttcggatc
    31021 tgagggccat gcgacctgca gacactgccc gtaagaggca ggccgccgca tctccactgc
    31081 agctgaggcc ctgtgccttt gcacagaccg gatggactgt gtataagcta gagctgtcat
    31141 gcgtcgcatt gtgcgcgcct ttttcacatg ctcacttctt cagtccgtac cgcgccatcc
    31201 gttgcgtgcc aatcaccaac gtcgtgcggc cctactcctg ctctggttgt ttctcttcgg
    31261 tccaccgctc aacgattcga tcgccgacgg acctgcagcg ccatccaagg gacagacgac
    31321 tcccatcacg ttgcgtttcg tctcctggaa acccgatcac ccgcgcgtgt gggacgacgc
    31381 cctcgacgaa ttcacgaaag ctcatcctca tatttcgatc gtgcgcgaac tggccccgca
    31441 cagctcgacg gcctatcacg atctcctgac gcagaagctg aagaatcgtg atgccacggt
    31501 cgatgtcttt ttcatggatg tcatctgggt gccggagttt gccgaagctg ggtgggcccg
    31561 acggctcgac gagcgattta ctccagccat gcgtgaggag tttctccccg ccaccatcga
    31621 ggtgggtcga tactccgacc atctctacgg cgtcccgagt cgcatcgatg ccgggctgct
    31681 ctactaccgc tcggatttgc tggccaagta cggcttctca ccaccgacga cctgggacga
    31741 gctcgcccga caagctgacg ccatcgtcgc cggggaacga cggaacaatc cgaccctgcg
    31801 cggctacaca gcacaattca aacagtatga aggcctcgtc tgtaacctgc tggagttcat
    31861 cgacggccac ggcgggagtc tgctgacgtc ggatggaact cattccactc tgggcagacc
    31921 cgaaacgctg gccgccgtgc agttcgttcg tgaccgggtg atcggccggc ttgcctcacg
    31981 cgcggcgttg acctatcaag aaccggaatc cctctccgtc tttcttcaag gccatgcagt
    32041 ctttcaccgc aactggccct atgcctggga actggccaac aaccggactc gatcgaccat
    32101 cgccggacag gtcgcggtga tgccccttcc cgggttcact ccggggcgca cggccgcagc
    32161 actcggcggc tggctctacg gcatcagcgc ctactcgcag catccagacg aggcctgggc
    32221 gctcatcgag tttctctcca gccaggccat gcaaaagaaa ttcacccagg aggccggcat
    32281 cgcgccctcc cgtcaggcgc tgttttccga tcccgacctg ctggcagcag cgccgcaact
    32341 ccgtaaccac ttcgccgtcc tccggtccgc aaccgctcgc ccgcgttccc ccctgtaccc
    32401 cgccatctcg catctactgc aacggtattt cagccgcgcc ctcgccatcg acggcctcga
    32461 cctcgcgcag gaagcggccg tgaccgacgc ccacatcgat cggctactcg ccctcacgag
    32521 gattgcgcca tgatccgccc ctcaccgatc ctgcgacatc gcgacagcct ggcggcctgg
    32581 acgatggtgg caccggcact gctcgtaaca atggtcttcg cactgtaccc cgtgttggat
    32641 tcgctctggc tcagcctgca caatattttc atcgggctgc cgcagctcgg cagtccgttc
    32701 gtcgggctgg acaattatgt ggcgctgctt cgtgatccgg tggcgcaaca ggcactagtc
    32761 gtcacccttg cctttgtggg cctctccacg atactcgaat tggcctgcgg gctgatcatc
    32821 gcgctggtga ttcatgagcg atttcgcggg cgcggtctcg tgcgggccgc tatcttgatt
    32881 ccctgggcca ttcccacagt cgtcgcgtcg caactgtggc gctatatctt caacgaccag
    32941 tatggcttcg caaacttatt gttgttcggc gaacgcgtca ccgactacgt cccctggctg
    33001 gcctatccgg gcgtggcctt cggcatcgtg gtgctggccg acgtctggaa aacctcctcc
    33061 ttcgccgccc tgctgatcct ggccggcctc caggtcattc ccgacgatct ctatgacgca
    33121 gcgcgtgtgg atggggccac cgtctggcag cggtttgggc acatcaccct gccgctgctg
    33181 aaaccggcac tgttgctcgc gctcttattt cggaccatgg atgcgtttcg tgtcttcgat
    33241 ctggtgttcg tgatgaccca aggcggcccg ggcgacgcca cgcaggtcct gcagttctat
    33301 ggctaccaaa ccctcttcac ggaaggccgc atcggatacg gctctgccgt ctccgtggtc
    33361 gtgttcctga tgatcctggc gctgtcgttg acgtacctgc gcgccatcgg ctcgagtctc
    33421 ttggagcgca ggcgaacatg aatcgtcgga tccttctcgc actcggcatc ctcggtacgg
    33481 tgggactcag tctgctgccg ttcctctggt ttgtcctcac gtcgttcaag tcgcaaggtg
    33541 agatcgaagt cgcgccgccc gcctggtggc cctcaggcaa cctgggattc tatcgctcag
    33601 ccctcttcga ccatcatctc ttcgactatg tgctgaacag cgtcgtcgtg gccggctcca
    33661 caactgtgct ggctctcctg ttcgccatcc cggcagccta tgcattagcc cggctgacca
    33721 tccccggcaa acaaggtatt ctggccgtgc tgctctgcgt ctcgatgttc cctcaaatgg
    33781 ccatcgccgg gcccgtctgg cgactgctcg acacaatcgg cgggctcaac caccgctggg
    33841 gcgtcgtgct gccctatgtg gccctcaccc taccgttggc gatctggatt ctggccagct
    33901 tctttaagga attgccgccg gagttggaag atgcggcacg agtggatggt tgcgggccct
    33961 ggacaactct gttccggatc acgcttccgc tggcaacccc ggggattttc accgccgcga
    34021 ttctgatctt gatctatgcc tggaacgagt ttttcttcgc cctcctgatc ctgacccagc
    34081 cggagcaaca gacccttcct gtcggcatcg ctctcttcca gggagaattc accatgccct
    34141 ggggagagct ggccgcagcc tccgtggtgg cgacccttcc actaatcgtg gtcgtgctgc
    34201 tatgtcaacg atggattgtg agcgggctat ccgccggggc ggtaaaaggc tagcaggcta
    34261 tccagaacga ccgccaactt cgttctcggt cgcacgtctc cctgcaacgt acccggaggg
    34321 tacgcttcag gcgccgtgct tcctgcggcc ttgctggacg gccgttctga atggcctgct
    34381 tgtgaacacc atgcgattcg aagggaacca tcatggctga attggaactg cggggtgttt
    34441 caaaatcgtt tggtgaccag cccgtgctgc gcgatctctc actgcaggtt ccggatggga
    34501 gcttcacgat tctgctcggc ccctccggtt gcggcaaatc gacgctcctg cgcatcatcg
    34561 ccgggctcga tagccagacc gccggccagg tgtgcatcga cggtacggta gtcgatcatc
    34621 tccccccagc cgaacgcgac atcgccatgg tctttcagca gtacgcgctc tacccgcacc
    34681 tttcggtccg cgagaatctc gcctttgccc tgacgatccg acgaactccg cgccgagtga
    34741 tcgacgagcg catcgccgaa gccgcgcaac tcttggacat cgagcctctc ctggaccgca
    34801 aacccaaaga tctgtcggga ggacaacggc aacgggtcgc gatggggcgg gcgatcgtgc
    34861 ggaaaccgaa gctgttcctg ttcgacgaac cgctctccaa tttggacgcc caactccgca
    34921 ccacgatgcg tatcgaactg aagaagttac aacagcgcct gcaggccacc atgatctatg
    34981 tcacccacga tcaagtggaa gccatgacgc tgggcgaccg catcgtggtc atcgagggcg
    35041 ggcgcatccg ccaagctgat gagccccatc gcacgtacgc cgcaccggcc gatcccttcg
    35101 tcgcctcctt catcgggaca ccgccgatga acctctggga aggcgccctc atgacagaag
    35161 gcggcgaaac cgtctttcgg agtcatgacc tgaccctccc gatgccggcc aggctgatcc
    35221 cacctgattc gacacagaat tccgccgtca cgctcggcat ccgcccggaa gacatccagc
    35281 ttcgggagac gcagacagca gtaccactga gcgggttggt ggaactggtg gaggatctcg
    35341 gggcggacct acttctgcat tgccgagccg gtgagcttcg cctcgtggtc cgtacggccc
    35401 gccgcaccga caaaattcag agacaaacag tgaaactctt ttttccagtc gataaactgc
    35461 atctcttcat cgaccaccgc cgattcgatc cgcctaccac cacgcccact tgactgtgcc
    35521 cctggctctc ttatctcttt cacaaggaca gcagcggcgg aagagaaaaa ccgtgagttc
    35581 gcttgcaatg catccggaga cctcgtaagc tccggaaagg attgcactct tcgcctgcgc
    35641 gcatcgaatt atcgtccacg gacagacctt gatggccaca tctcaacgcg gttactacga
    35701 catcctcggc gtgccacgga acgcgacggc cgacgacatc aagaaggcct ttcgtcgccg
    35761 tgcccgtgaa tttcatccgg atctccatac cggcacaaaa aaaacggaga tggagaagaa
    35821 gttcaaagaa ttgaacgaag cgcacgaggt gctgtcggac ccggacaagc gaaaaaaata
    35881 cgaccagtac ggctcgaact gggaacatgc ggaagcctat gagcaggcgc gccaacaggc
    35941 cggcgcccaa gcgggacgtg gcggccaggc cggcggattc agcggcgact tcggcgatat
    36001 cttcgagacc tttttcggcg gacgaacccg cggcggcaca tctccggggt ttgccgtcga
    36061 tggggaagat ctggaaaccg atgtccatct caccatccgc gacgtcctga ccggggtcag
    36121 ccggcgcatc gatctcaccg aacgcgtcgt atgcaaggcc tgcggcggca gcgccatcgt
    36181 acggggccgg ccctgtgtcg tgtgcggtgg tgccggcacc caagctgaaa aacgcacgat
    36241 cgaagtacgc attccggccg gtgtgcagaa tgagacccgt gtccgcctgg cgggcaaggg
    36301 acaacccggc atcaatggag gcaaaccggg agacctctat ctacgcgtcc atatccaggc
    36361 caacggcgtc tttcggcaaa agggattcga tattcaggtc acccttcctg tctggccatg
    36421 ggaggcggcg ctgggcgctg aagtgatggc tccgaccctg acggaaccgg tgaaggtgaa
    36481 aattcccccc ggtagcaaag ccgacagtaa actgcgcctc aagggcaagg ggctcccgac
    36541 ctcgaccggc gagcagggag atctcttcct gaagctgaaa atcgtcatgc ccaccgccat
    36601 ctctgacgaa gaacgggcgc tctacgaaca attgggccgc gcccgccaca gcgatccccg
    36661 ggccgagatc atcgccgcat cgagacgcag ttcatcctga gtagcgccgc cccaggccaa
    36721 tcccttctat gactatcgcc gaatacgcgc tcctcgcgtt cagttcgctt ttcgtgatcg
    36781 tcgatcccat cgccgtggtc ccggctttcc tcgccatgac cccacgggat tccgtggcgc
    36841 aacggctccg gaccgcgcgg gtcgcctgtc tggttagcgt cgccctcctc acgggattcg
    36901 ccctattcgg gcaaaccgtc ctcaaattgt taggcattac cttgccggcg gttcagattg
    36961 ccggcgggct gatcctatta cttgtggcgt tggacatgct gcgggcgcaa cggtccacgg
    37021 tgcaggaaac ggctgcggaa acagcagccg gcaccaacaa ggacgacatc gccatcaccc
    37081 cactcgccat ccccatgctg gcggggcctg ccgcgatctc gacggtgatc ctactggaga
    37141 cgcaggccac cacattcatg ttgcacgtcg tattattggg atgccttgta ctcatcgggt
    37201 tggcgagtta cattatatta gcgatcgacg cacgcagcgc gaagtggctc aatcccatcg
    37261 ccgagaagat catctcgcga ctcatgggct tattgctcgc cgcgctggcc gttcaattta
    37321 tggtgaatgc cctcaccggg gaccaagggc tgttacgcgg gttgaccgga cattaatcgc
    37381 tctcaactga aggaggaacg aatgacgacg aaactgttca catttgccac cgcttcggcc
    37441 atcgcctgcg cgctgacgat ctcccctgcc tg
//
LOCUS       DAORRQ010000043        35933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_72_length_35933_cov_63.454122, whole genome shotgun sequence.
ACCESSION   DAORRQ010000043 DAORRQ010000000
VERSION     DAORRQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 35933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35933
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_35933_cov_63.454122"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /gene="tdh"
                     /locus_tag="PK224_13745"
     CDS             <1..188
                     /gene="tdh"
                     /locus_tag="PK224_13745"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="converts threonine and NAD to
                     1,2-amino-3-oxobutanoate and NADH; functions in threonine
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRB16854.1"
                     /translation="GIRVHGITGRRLFSTWYRLAGLFKAGLNIRPVLTHTFPLKEFAQ
                     GFELIKSGQCGKVVLFP"
     gene            272..1462
                     /locus_tag="PK224_13750"
     CDS             272..1462
                     /locus_tag="PK224_13750"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRB16855.1"
                     /translation="MAYTSLKTAAEQQLAEIRAAGLYKTERQILSPQSTEIQVAQGDV
                     VNLCANNYLGLANHPDVRQAAADGLREFGYGMASVRFICGTQRLHKTLEAAISTFFGT
                     EDTILYSSCFDANGGLFETLLDERDTIISDALNHASLIDGIRLCKAARLRYAHADMAE
                     LETRLVESASSRVRMIVTDGVFSMDGDLAKLDRIVELADGYDAAVVVDDSHATGVLGK
                     GGRGTPDHFGVAGRIDLVTSTLGKALGGATGGFTTGRKELIELLRQRSRPYLFSNSLP
                     PVIAAAALKAIELVERGDDLRHTLMEQARWLRGQLTALGFTLVPGTHPIIPVMLGEAT
                     VATQMADRLLQEGIYVVGFSYPVVPKGQARIRLQLSAAHTRLQLERAVAAFAKVGREL
                     NVIG"
     gene            complement(1467..2447)
                     /locus_tag="PK224_13755"
     CDS             complement(1467..2447)
                     /locus_tag="PK224_13755"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRB16856.1"
                     /translation="MVHRIGVNARRFVRVVCAGVVLTVVGCAGGPSQTTLNTSVGVES
                     AARAESRGEGAETVQVAAAPAVSPNVILAANKSAPSAATEPTVEPAEDPFYDPFAKSD
                     EPVGGEEYDPWEPVNTKVFEFNRQVDRWVLKPAAQGYNAVVPNPVQIGISNLFYNTRF
                     PSRLINNLAQGKFSGAGTEVGRFLLNSTFGLGGLVDVAKYMNITTPEEDTGQTLGYYG
                     MKPGPYVVLPFLPPFTLRDLVGYIGDIALNPINWMVFPIIEVNGIPSLVAHHNRTTSS
                     IAQIGGRVEEILNDRSLNLEKFQGVEEATLDLYTAVKNAYIQKRRNAIRE"
     gene            complement(2671..3432)
                     /locus_tag="PK224_13760"
     CDS             complement(2671..3432)
                     /locus_tag="PK224_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB16857.1"
                     /translation="MSLLDQFFVYHPHPWEEGDWSAVGGVPLEDVWFQAGDGTKLFGW
                     YAESSATTAVLLWCHGNAGNLTHRLDNLRALYRLGLSVFLFDYRGYGKSQGRPSEDGL
                     YQDAIGAYDYLTRVRRIRPERLVIFGRSLGGAVAGELATQRPAMGLLLESCFPSIEAV
                     ARHHYMGLPVHWLLGAAFRLEDRLSQLSLPKLFVHGDRDDIIPIELGQRAFVAAKAPK
                     EFYIVRGADHNDVPSVGGRAYFAKLSAFIAAALGR"
     gene            complement(3447..5372)
                     /locus_tag="PK224_13765"
     CDS             complement(3447..5372)
                     /locus_tag="PK224_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRB16858.1"
                     /translation="MFSSLRSIVHILSLLLAGLFLATSVAAEDAPAPYLTQLLQQARQ
                     ARLADAREWHLLLHYRANLFGGYTSEQDDPGFFLAPDGKTNPEVELDATLRQFFSPDL
                     VGRSKQPAQCAFVARYAWLKERLNVDPSQLPPMPCERFERWIDEFHARSVTLVFPSAF
                     MNNPASMFGHTFLRIDQEGQTAQTRLLAYTINFAADVPPDEGLAYPVRGIFGGYRGQF
                     STVPYYLKVQEYRDIENRDIWEYRLNFNEQQIRRLLMHAWELRHASFDYFFFKENCSY
                     HLLSLLEYADPSLHLTERFRFWTVPADTVRVLAAEPGLVNEIAFRPSRVTLIRRKREH
                     LSDMEQGLVKRLVRDGSIIESEDVRGLPLSRQAVVLDIASDYALYISERDEAQVAISR
                     ERNRQILTARSLLRIPSEDLKILPFAMQPELGHKTSRVSVGGGWRNNDTYEEVSLRTA
                     YHDLLDPEPGYTPGAQIELGSISLRHYNRADQTRVERATLANVLSLSPMDSLFHAPSW
                     KLNMGMQTITHGGCELCSNWHVNGGIGAATETHLLRHEVLFVFAEVEGNYSRAYEERH
                     RVGGGASAGFYADLTERWRMLASTTYLRYALGDKSDDVRWSVAQRYTVAQNLALRMEY
                     NHRDHDNDVLFTVQAFF"
     gene            complement(5405..5911)
                     /locus_tag="PK224_13770"
     CDS             complement(5405..5911)
                     /locus_tag="PK224_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HRB16859.1"
                     /translation="MLRKLFVVLGAVAMVSQAGLVMAANPDTGPGCGLGKLAWADYKN
                     QKNIAPQAFMALTNATFMNTFAISSGTSGCTNDGQVMADQKTTMFALLNFENLTQEMA
                     QGKGEHLASLAALMGIPDEQHPAFFALTQERYSDLVQAGETSSVALVKTLNDAVSRSP
                     VLAQVVSR"
     gene            complement(6039..6524)
                     /locus_tag="PK224_13775"
     CDS             complement(6039..6524)
                     /locus_tag="PK224_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRB16860.1"
                     /translation="MRIGVVSDTHGVFDRAIVRHFAGVDRILHAGDIGRREVIEQLEA
                     IAPVTVVSGNVDGFEASGFPVEQVIELAGHRIALHHRLYEKGKLTRDGAHFLSRTNPA
                     ICLYGHSHQPKAEWRDGVLLFNPGSAGPKRFHLPRAIGLLLLDGEAIDAQHITLADRA
                     E"
     gene            complement(6840..7334)
                     /locus_tag="PK224_13780"
     CDS             complement(6840..7334)
                     /locus_tag="PK224_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16861.1"
                     /translation="MKKLRLIAPLLFSVAALASGCIAQHSNTLSAPMQVRLTANELKA
                     DVAVGEKISGHAKITYFLTIPFGSSNKYADGVLYDGAVVSQLGVYNPYAVQPGDFFSS
                     LFSLFSVGAHEAASQAAYDAISKSGADIIIAPRYVTDVQDFFIFKNVEVQVTGYKGTV
                     KGIH"
     gene            complement(7555..8688)
                     /locus_tag="PK224_13785"
     CDS             complement(7555..8688)
                     /locus_tag="PK224_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRB16862.1"
                     /translation="MNPTPNALCFGGGLPGAGAPCYVTVSADGLTMASVGDSPLLEEA
                     IAFTAMSVEAGGLDHDQLVVSWGVGASVRTLYLKDPALIIAFRRAAPSALMAHLERAA
                     EQVRRARHSHRVLWGSAVGVVVGLGLMVWFGSDLIVEWAVARIPVQWEQKLGETVYQD
                     FLSKETVLKDGPAVSAVQEMTQRMTAKIPNNPYTFQVSVVQSPMVNAFALPGGYVVVF
                     TGLMRKAESGEEVAGVLSHELNHVLQRHGMERLVKMMGVAAVVSILVGDQQGLIGLAR
                     QLGLELATLKFGREQETEADVTGIRLLSDARIAPDGMIRFFERLSEKDKERVELFSTH
                     PMSAARAERLKTELAALPKQTPEPFTFEWKQVQDSLSSSEQRK"
     gene            complement(8826..9509)
                     /locus_tag="PK224_13790"
     CDS             complement(8826..9509)
                     /locus_tag="PK224_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HRB16863.1"
                     /translation="MKSEILYPGAFPMVRVYLNEGETVKAESGAMVAASPTIDIESKM
                     EGGFLGALSRKMLSGEKFFFQTLRASRGAGEVLLAPTVPGEIVLLDLDGVNEYMVQKD
                     GFLAGAEGVKIESKMQSLTRGLLGGEGFFILKIGGTGQLILNSFGAIHKIELKPNEEY
                     IVDNSHLVAWTSTTTYNIEKASSGWIASLTSGEGLVCRFRGPGVVYIQSRNPGSFGNW
                     IRQFIPVSE"
     gene            9768..10103
                     /locus_tag="PK224_13795"
     CDS             9768..10103
                     /locus_tag="PK224_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16864.1"
                     /translation="MKRVVIEELTTGMILAKPVINNAGLVVLPVGAELDEATLSRLQR
                     LGLTSVYVEGEAGDASGKTLDELEAELDHRFRLVAQDPIQCLLREAIRLHLRTTHGIS
                     DNPEATPSA"
     gene            10100..10936
                     /locus_tag="PK224_13800"
     CDS             10100..10936
                     /locus_tag="PK224_13800"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRB16865.1"
                     /translation="MNPSRKIDLHRRIEQVGELPTLPHVVQKLASMIGRPNVSAEEIG
                     TLLEKDQVLSAKVLRLANSPFYGFPSRIASVAHAVVVLGLNVVKGLTLCATAFDMMRN
                     AGMNELWRHSLGVAMTAHILGTKAAMKNPEEVFVAGLLHDIGKVVLYVKWPDVGQQIT
                     TVTRNTSRPLMDTEQELFEVTHADVGGWLASAWHLPTSLREPILHHHTPAAAQDAKLQ
                     TAIVHVADVLVKGLACGNPGDDLVPPLSRQAWDLVGLDAQSLAHCLTQATEEFQTIDD
                     YL"
     gene            10933..11907
                     /locus_tag="PK224_13805"
     CDS             10933..11907
                     /locus_tag="PK224_13805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRB16866.1"
                     /translation="MSGSPQNRRILLLHSDPLEIERLTAGLNRSGFTVVAADAGRLAM
                     HELVHDPPCLVLAAEGTNGRSADTLARDLRADPFLGRLPLIILVRDIRVNDLDWATLG
                     VDDYIAVPYRPDEVPQRVRLCLSRLERSLDANPLTRLPGNSTILHETTARIESRAPFA
                     LAYLDIDNFKSFNDRYGYARGDEVLVVTCRILTTVVSELAGTDGFVGHVGGDDFVFMS
                     APSAIDAICQTLIKRFDLVIPDFYDPEDRQAGFIDSVDRRGNHERFPIMSLSIAVVTN
                     EHRDISHPGDVSKIASELKKVAKSRPGSVYVKDNRTNPPDSAPESTPR"
     gene            complement(11959..12465)
                     /locus_tag="PK224_13810"
     CDS             complement(11959..12465)
                     /locus_tag="PK224_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HRB16867.1"
                     /translation="MRALLVCVIVLACGVASAGAQGDSAGVPWSQLSPGEQQLLQRFS
                     DNWDQLPPRKQQRLRNGAQQWGTMTPEERQEAKQRFKQWRTLPPEQQQQLRERYQQFR
                     QLPPEQRESVRDARRWFRSLPPEQRKELREKFKHMSPEERRAYRRELRRQYGGNVGGQ
                     APGGSSAP"
     gene            complement(12766..13125)
                     /locus_tag="PK224_13815"
     CDS             complement(12766..13125)
                     /locus_tag="PK224_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16868.1"
                     /translation="MNERSDEQRDPLAVAAKHLLDRSVQDLDQKTVLSVQRARLAALD
                     AASRRRQPWLRWAGGVALASMATLMVTIWLWQSNGANHSHLLLEDLELMQSPENIELS
                     EDLEFYNWLADGTATTG"
     gene            complement(13122..13739)
                     /locus_tag="PK224_13820"
     CDS             complement(13122..13739)
                     /locus_tag="PK224_13820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB16869.1"
                     /translation="MNGCHSQIAVDGQPGSLCLGERVGSLDRTQALDRFLAGIERRAF
                     RMAHIATGNKDEALDLVQDAMLKLAHKYGERAEEEWGPLFHCILQSRIRDWYRRERVR
                     NRLREFFRSPHDEEEGEDPLEQIADSTAVAPDEDVQRKRACAELDVALRALPLRQQQA
                     FLLRIWEELDVAQTAQAMGCSQGSVKTHLFRALQVLRTRLGAHWP"
     gene            complement(13736..13993)
                     /locus_tag="PK224_13825"
     CDS             complement(13736..13993)
                     /locus_tag="PK224_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16870.1"
                     /translation="MTIQRREEGMDERMVGDAMVHCLPFLDFTVVRVENRTDLRFPGA
                     TRICVRRSAQYGTCPQQAVLVSAGIGDCGLAGCRVEVDRRE"
     gene            complement(14118..19016)
                     /locus_tag="PK224_13830"
     CDS             complement(14118..19016)
                     /locus_tag="PK224_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16871.1"
                     /translation="MRSDKKSPLKSDKKVPPKVQKKGSIGFNKKSALLEDAITQMNAG
                     KYGRASSALKDLLALDPLNAEARRLFATLHLRLGSLMSARTAFESLAREAMERQDYWL
                     AESLLREYLTAGPRYVPFLEMLGRVYEEKGDVMAAVAEYGKAVEVLLEDPDTDHPNRA
                     SELFARIRSLAPGSPVAFRFAAMFDTVTGQVLQVTPQPPVVDSISETAHAPVLTETSQ
                     GTDSAVVMPWEQVETASSDVPRPQEIQVSAVESPEVIAFTTTPSRLEAVTEGLSQARP
                     EEPLSVTLPVDSPQESEVQLQSESSVSAVTSAVEPVQVAGESDLPVLDVALVSTEPAE
                     LVPASVAEVSLPAIDASVKTGLDSIATPLAQPAPMPWDQVEEAAGIIPSQPEGPAASP
                     AGIAPTHSGETAPTPSVAELAGPTIEVLTQSSAHPIATAPASPAPMPWDQIEEVAVVI
                     ASPPSSPEPVMVEPESLPPTQNEVSTLPLAGVAPFQPVEPEPPAAAALMPFEEMDLQA
                     PVSEPIAPTIEVLTQASSQPIAQAPTSPASMPWDQIEEATDAVDPRTETLVEPGTDEV
                     EKAIESPAPNHAVEVSQAAESVPSSRPSELTSSGLSWEEILAAVAAMQTSPAPAQTLS
                     DGEQKPAVDAAVQEQTAGALTAPPASPEEESPLFEATVPDIPADQAGTATTLSAPMPW
                     EQIEVDDVTIPRQEPEPEFGPAFAEGAVSVQDSTVALPAAPDVTEATVAREACLAETV
                     ADRPLEPIATVTAELRILSPDAPTEAPEDSIRIPVEPYKAVEVPTPDAVPEAEQPAES
                     TIDRAVEPPLRLADSESAVATSSGEPPAASVEPVTEFAIAVSTDVTSMEPEQVTVEEP
                     QVPVGEPVAELPLEPVLLVEQPAEILYAQEVIVELAPEAVAESRVPAATPFLADEKPV
                     AGAEPVTPDAPLSSVVLEVTPQAEPEMALTDQEPSSPSAAEVREVDTVPISEFIVPPV
                     EASLNQVAPPPEAVSLPRGPLPIVVAPEEPVLPPVEPTQAAVVSPSPELVETPAESVA
                     PSEEQAPAPMAQVASPPDAGEGLRILWDDTSSKPTPSASTRNMLTRWLKKRAEPVPAE
                     VAEATPPATVSDEAPAPVPALSVEHQEPAVVAVERPPDEQETVAPMPVDAPVARPKPS
                     QPVGAGAWTRMGELVASLVGAGVSTTQSLVVMVLALVGLTLVLIGGAVGVVALTWLVL
                     EEQPSAAYRTMTSVPQRTLEDSSRNGYVLLLGFGAAASQDPVQAGMDRRVEGADRAYA
                     HTCLTGEGVFSGGDQGVSAESVGKWMKTADPAAQMRADAIGVKGWASRAEVSMGRYRQ
                     WLTKPFEDWGYGQSITPNCGLILYAHRLYIAEGFAQDVEAGVTRLESDLTAWRTVLGQ
                     AKTLPVKMLASAAMNDDIVVMSGLLLRPELDDRFVSRLAKLARPLEQAEQSVRWPMQS
                     QFVLATKTLDEAVSEDRSDARPFYGPVAAALPLPKQRRFNAYAQYYEAAGKAAAEGRY
                     TDLPKQSQFVHAPPYGLGDIVMNPIESLVGVDPLPTWETYAGRVLETDARLRLASLQG
                     WLRRTPPEQDLLTRIAKAGQGVYDPFTGFPMLVNMKKGVLYSVGQDLKDNEAQDRFDL
                     VAPIPSVAWAGGKRPADADKSK"
     gene            19426..20256
                     /locus_tag="PK224_13835"
     CDS             19426..20256
                     /locus_tag="PK224_13835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRB16872.1"
                     /translation="MTKAHILILTDLDGTLLDATTYSYEAATGALSAIQTLGATLILV
                     SSKTRSEMEPLRLRLDNRHPFIVENGGALFIPMGYFPFPLEQAAPRGDYEVVEIGTPY
                     VRLRTALKEIGRDLGCRLRGFGDLSLEEVSDLTGLSPAETLLATQREYDEPVVVESNG
                     MAWNRLSAAAATRGLRWTRGGRFYHLMGGNDKGVASRRLIAWYERRAQQEGRALITVG
                     IGDSLNDLPMLEAVTYPILVRKPDGSYDSDVQFPHLTRAEGVGPVGWNRSLMDLLPTL
                     "
     gene            complement(20318..20539)
                     /locus_tag="PK224_13840"
     CDS             complement(20318..20539)
                     /locus_tag="PK224_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16873.1"
                     /translation="MTDKISDVDAEAKARRWLGGEAAVPRYPHRRQSPFVFNRSLVGL
                     VAFAVGLGFAGGLLAGLQCRRRPPVSAKE"
     gene            complement(20586..21293)
                     /locus_tag="PK224_13845"
     CDS             complement(20586..21293)
                     /locus_tag="PK224_13845"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16874.1"
                     /translation="MKEFFEKGLLDPLELMARQVLAVLPSLLAMSIIFFAGLVVAWTA
                     SQALERLLRVVGLDRLFDRLGVTGALLRGGVKTDPSRLVGQAAYWLVMIFATVAALGA
                     LNLQPINDFAKSFLAYVPHLVTAGLILVAGWLLSNFVSQAVLIAAVNAGLPPARLVAT
                     CSRWGIQLLAVAMALEQLGIAQNIVVVGFGITLGGLVMAGAIAFGLGAKDLAKDYLER
                     GLSIRTRDGAPDDLRHL"
     gene            complement(21351..22652)
                     /locus_tag="PK224_13850"
     CDS             complement(21351..22652)
                     /locus_tag="PK224_13850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB16875.1"
                     /translation="MTPGPQLPDATHTTPLTPETEAQVDQFAHTDILVGIPSFNNVET
                     IGHVVKAVSAGLAKYFPEARAVLVNSDGGSTDGTQQVVSQAVVDLKTLFIGDQQSSLH
                     KIITPYHGIPGKGSAFRTIFEIARRLKAKACAVVDSDLRSITPEWIELLVSPVFEQGF
                     DYVAPYYLRHKYDGTITNSIVYPLTRTLYGHRIRQPIGGDFGFSGQLAQHYLDKHVWE
                     SEVAKFGIDIWMTTEAIASGARVCQSFLGAKIHNPKDPAADLSAMLVQVLGAVFALME
                     DHYAVWGKTDGSNAVPLFGFQYEVGVEPVNVNVDRMISTFRQGLSDLGTIWDQILAPG
                     TRQSLRPLGTCSAQDFRIADNLWARVVYDAAVAYRNRVLPRDHVLKAFTPLYLGRTAS
                     FVLDTQGLTSSEAEGRIEALCQAFEKDKSYLVARWNEPHAG"
     gene            complement(22952..23317)
                     /locus_tag="PK224_13855"
     CDS             complement(22952..23317)
                     /locus_tag="PK224_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16876.1"
                     /translation="MEQQNRPRFIVECTGSRSEDGLLVWNGRMLDLSIPGWSRTGLKG
                     LQAGDLLQLHLHIPGQPRPLSVRLATIRWASESKLGVDPILMDADDQLRLNDFVSAHD
                     TTPPFSTDRKEQIVISDAG"
     gene            complement(23542..23886)
                     /locus_tag="PK224_13860"
     CDS             complement(23542..23886)
                     /locus_tag="PK224_13860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB16877.1"
                     /translation="MAKSQTSRSHSRVPVSCFLYYLGEGLVGTGKVCDLSVKGWRIDG
                     DKPVTVGMKLTLRVFLPDQPKAIDVDGVTVQWVQGRVFGLETVRMSATAQARIHKFVE
                     SVLKESGSSRVA"
     gene            complement(24050..24424)
                     /locus_tag="PK224_13865"
     CDS             complement(24050..24424)
                     /locus_tag="PK224_13865"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB16878.1"
                     /translation="MTGGNKQVAPLAPRSSPAPVATTFQLRSFRRFPVQCSVYYSSDA
                     LQGTGVAWNLSLNGWRIDGTHPVEPGTVVTLCVFLPDQHPTVFIDRAVVRWSRGQEFG
                     IEVNEIKADEHARLEQFVTALV"
     gene            complement(24462..24538)
                     /locus_tag="PK224_13870"
     tRNA            complement(24462..24538)
                     /locus_tag="PK224_13870"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24502..24504),aa:His,seq:gtg)
     gene            complement(24707..25426)
                     /gene="pyrF"
                     /locus_tag="PK224_13875"
     CDS             complement(24707..25426)
                     /gene="pyrF"
                     /locus_tag="PK224_13875"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRB16879.1"
                     /translation="MMTRIDARDRLIVALDVPSAAEADALVARMGDQVRFVKVGLELY
                     TVAGPDIVRKLVDRGKRVFLDLKFLDIEETVRRATARVAAMGATFLTVHANRKALIAA
                     VQGRGSSDLKLLAVTVLTNFDGEDLREMGIQRSVQDLVTARAMLAAEVGCDGVVASGE
                     EPQAIRAKVGPNLVIVTPGVRPAGKGTDDHARVTTPTQTIAAGADYLVIGRPIRDAQD
                     PPAATAAILEEMQAAFDRQGR"
     gene            25541..25816
                     /gene="rpsT"
                     /locus_tag="PK224_13880"
     CDS             25541..25816
                     /gene="rpsT"
                     /locus_tag="PK224_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRB16880.1"
                     /translation="MPVVHKSTIRRARQSEKRRLRNRSTISSVRSILRKVQDAIAAKK
                     PDEAKATLREATSALSKAVTKGVLKPNTASRRVSRLTVRVNAITASA"
     gene            complement(25800..26909)
                     /gene="holA"
                     /locus_tag="PK224_13885"
     CDS             complement(25800..26909)
                     /gene="holA"
                     /locus_tag="PK224_13885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRB16881.1"
                     /translation="MSASVMSLELPQSLARSGVMPLYAVVGEEDYLRDQSVAALRAAA
                     LGPAADTGFNYDIFHGDDCSVEDVLACAAEIPVFAERRVVVYKSVEKLPAREGEKLLS
                     YFSAPNDTTTLIVVGVKLDGRMKWTQALTKGAVTVNCAPLRDAQLSAWIRQEAAALGV
                     RIHEEATQLLKEVGSESLYAVKRELEKLAAYVPPDRTVQPADVEALRGTESGASVFDL
                     MNAIGAHDHGRALRILARNLENGEAPLRILGALVWQYRRLWKMKEQLKAGGREGEAAR
                     TLRMDPSRVRGFLAQFSDVQLTHTFRWFLETDSKLKGGSGSAPVRVMEDLLFRLCGQP
                     SKSASSAARVQPAPPVPRPSGSRPVSNVRTVTRKR"
     gene            complement(26930..27556)
                     /gene="lptE"
                     /locus_tag="PK224_13890"
     CDS             complement(26930..27556)
                     /gene="lptE"
                     /locus_tag="PK224_13890"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRB16882.1"
                     /translation="MVWLIIFFALSVSLPGCGYQFRVQGAGPTVGGAPEAATTRPHAP
                     RLAILPIANSTYEANFEVKLANYLRREFSAGAGAEIVGPSAGADLYLSGQIMQITLPT
                     LSFDQTTTFESRVEMLVSVRIEEAKTKKVVWYQVAKGTSEFFLTQDLQFNRVLQNRAL
                     EQAGRFVAEDLASRFLLFLDTGELDKALKRPVKAEVAPAGGTPPYGAR"
     gene            complement(27624..30059)
                     /gene="leuS"
                     /locus_tag="PK224_13895"
     CDS             complement(27624..30059)
                     /gene="leuS"
                     /locus_tag="PK224_13895"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRB16883.1"
                     /translation="MSKTYDHRALESKWQTYWETHRPFRAVDDHSKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMRGFNVLHPMGWDAFGLPAEQYAVKTGVHPAITT
                     AQNIATFKRQMKRVGLSYDWERELSTTDQDYYRWTQWIFLKLFERGLAYVAEVPVNWC
                     PALGTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYAERLLEDLTLVEWPTSTLE
                     MQKNWIGRSIGAEVDFPLADKTGNLRVFTTRPDTLFGATYMVLAPEHPLVDVVTTPEQ
                     REQVTAYRDAASRKSDLQRQELDKVKTGVFAGGYAVNPVNQKRLPIWIADYVLMSYGT
                     GAIMAVPAHDERDWAFATQYHLPIREVIEGGQVDKAAFVETDRGRVINSTTPDGSCSL
                     DGLLPSEAIPKMTAWLETQGRGKKTINYKLRDWLFARQRYWGEPFPIVWVDGEPRPLP
                     EEQLPLPLPETKNFKPSGSGESPLANLHDWLETTDPVTGKPARRETNTMPQWAGSCWY
                     YLRFIDPKNPVQVVDPAKERYWMPVDLYIGGSEHAVLHLLYSRFWHKVLFDAGVVSTP
                     EPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEMVDQFGADAVRLYEMFMGPLESMKPW
                     STRGVEGITRFLDRVWRLMIGDEGALSPAVTDTEPTPEQLRLLHYTIKKVTDDIDALR
                     FNTAISQMMVFTNEMTKLEQRPRRLLEPFVLLLLPFAPHLSEELWERLGQQPSAGQQP
                     WPQFDPELVVSDRLTIPIQVNGKLRGKLEVDAGASREQLEPLAKKEVAEWLQGKEPKK
                     VIYVEKKLINFVV"
     gene            30316..30930
                     /locus_tag="PK224_13900"
     CDS             30316..30930
                     /locus_tag="PK224_13900"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB16884.1"
                     /translation="MDWVLRGERFQTERRLLLTPVHGVVLEIGFGTGLNLPHYPTTVT
                     ALHTVDPAPLLPDRVAVRVAQASFPVHIQHVSAERLPYDDASFDYAVSTFTLCTIPDP
                     ARALRDIRRVLKPDGHFLFLEHGRSDDPLIARWQDRLNPLQHVLACGCNLNRRIDRLV
                     LDAGLRLEQLDRYCLPGVPRIGGEMYRGTARLNGQSSLHSSLDT"
     gene            31090..35034
                     /locus_tag="PK224_13905"
     CDS             31090..35034
                     /locus_tag="PK224_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB16885.1"
                     /translation="MSSSQSFPDREPPSTSPLFKPIDSARDSSIALQRVTLLYEAMPA
                     ALVAGLGCALALIAVNWHVVPHDRLLVWLVYHLILAAGRYGLARSFTANKPGPANIER
                     WERAFLVGTTLAGLGWGASALLLFPESSPAHQVFLAFVLAGITAGAASTLAARFDAFL
                     SFSLPMLTPLILRLFALDHEQALPMAICTMLFSLLMVYTAYRTSNTVLETLRLKYHNA
                     QLVDQLTGQLQEGEQMELLLTVKTEHHRFIMEYAQDIIYRTDRSGRFTFINPAVIRLL
                     GYHETEMLGHRALDFVHPDYRRPTERFYLRQFLRKTASTYCEFPLVTRNQQTLWVGQN
                     VQLLQRDQEVIGFQAVVRNISARKQTEEALRVSQERYRALFESSPEMLLTVDARGTLL
                     TVNTTTAAELGYEADELIGQPVARIVHPDDHGSMDKHFADSLRRPGEVLRWNFRKMRK
                     DGSLLWVREAARAVRNSDGQFDIIIVCENVTEQKGIEDALTQTRQLLESIVEHIPHMV
                     FLKDAQELRFVQFNKAGEELIGLPREALLGKNDFDFFPLEQAEFFTARDREVLTGGTM
                     MDIPAEPIQTKDHGLRWLHTKKLPLYDQAGMPRYLLGISEDITDRKQREETERLRLGK
                     LETQQAVLHELAEDPAIHSGHPQQAFPAITEHAVNTFAVERAGIWLFGENQSALILQD
                     LFEATPKHHLQGTTLSTGEYPAYLHALETEPYSLAAHDAQTDPRTSELTPLYLTPLGI
                     VAMLDAPIRRHGRVVGVLCLEHIGPARVWTTEEQTFAASLAAMATLTLEASDRRQAQE
                     ALEQHVRERTGELRRTTAHLQTIIEESPLAMIELDQAGLVTTWNAAATALFGWTKDEV
                     LGKELPYVPPGQEEVSEDLWTAVMSGAAPRNLELRRQKKDGTLVDVNMWGSLLQGPAG
                     QAVGSIGFFVDITKHKQLEDQLRQAHKMEGIGRLAGGVAHDFNNLLTVINGCATLVLD
                     QVRAEDPLRRSLTEILTAGQRAAALTKQLLAFSRRQVLTFQVFDLNEALSSISSMIER
                     LIGEDINLIRNLDSRPCTIRADRGQIDQVVLNLAVNAKDAMPTGGTLTMATQALLITP
                     DSPVPHPALLPGAYAHLSIRDSGQGMDRTTLSHIFEPFFTTKEEGKGTGLGLATVYGI
                     VKQSQGFIFADSTPGEGSTFDLYFPLVEAPPLAAAPTLPSRPHCGRETILLVEDQHAV
                     RLLLTQALSDYGYTLLEASSGQEALRLVAAARTPIHILLTDVVMPQMTGPALAEHLRR
                     QWPELRVLFMSGYAEGSVLPTFLAEPGTGFIQKPFLPTELAQKLRELLDPAK"
     gene            complement(35081..35767)
                     /locus_tag="PK224_13910"
     CDS             complement(35081..35767)
                     /locus_tag="PK224_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRB16886.1"
                     /translation="MLSNRSEAPHQSVDWSRIDDVLLDMDGTLLDRHFDNFFFEEELP
                     RRYAAKHALTFEAARDRLMAMYRSVEGELAWTDLHYWSERVDIDVVAMHRELDHMIGF
                     LPDAEEFLSSLRRLGKRVTILTNAHRAGVDVKTAKTGLDRHVDRIVDAFEVGWLKMRS
                     EYWPTCRQLVGFDPARALYIDDDEQCLAAAEDFGIGRIFHRSRSSSQASAVPSARFTS
                     IEHFHAILPG"
BASE COUNT         7586 a        11502 c         9935 g         6910 t
ORIGIN      
        1 aaggcatccg tgtccatggt attaccggtc gtcgcctatt cagcacctgg tatcgcctgg
       61 ccggtctctt caaagccggc ctcaatatcc gtccggtcct cacccatacc ttccccctca
      121 aagaatttgc ccaaggattt gaattgatca aatccggcca atgcggcaaa gtagtcctgt
      181 ttccataagc gacaggggct agagttccgc gaaacggatc ggatatctga gcttccctca
      241 accgtttcac gtttcacctt tcacgaggaa gatggcctac acctcgctca aaacagccgc
      301 cgaacaacaa ctggcggaga tccgtgccgc cggtctctac aagaccgaac ggcagatcct
      361 gagcccgcag agcaccgaga ttcaggtggc acagggggac gtcgtcaatc tctgcgcgaa
      421 caattacctc gggttggcga atcatccgga tgtgaggcag gcggccgccg acggactcag
      481 agaattcggg tatggtatgg cgtccgtgcg attcatctgc ggaacgcaac gactgcacaa
      541 aaccctcgaa gcggcgatca gcaccttctt cggcactgaa gacaccatcc tctatagctc
      601 ctgcttcgac gccaacggcg gactgttcga aaccctgctg gacgagcggg ataccattat
      661 cagcgatgcc ttgaaccacg cgagcctgat cgacggcatt cgcctctgca aagccgcccg
      721 gctgcgctat gcccatgccg acatggccga gttggaaaca cgactggtgg aatcggcctc
      781 aagccgcgtg cgcatgatcg tgaccgacgg ggtgttttca atggacggcg atctggcgaa
      841 gctggaccgc attgtggagc tggccgatgg atacgatgcc gccgtggtcg tcgacgacag
      901 tcatgccacc ggcgtgttgg ggaaaggcgg acgcggcacc cctgaccatt tcggagtcgc
      961 cgggcggatt gacctcgtca cgagcaccct tggcaaggcg ctgggcggcg caaccggcgg
     1021 gtttaccacc ggcaggaaag agctgatcga actgttacga caacgttccc ggccctatct
     1081 cttctcaaat agtctaccgc cggtcatcgc cgcagcggca ctgaaggcca tcgagttggt
     1141 ggaacgaggc gacgacctgc gccatacctt gatggagcag gcccgctggc tccgggggca
     1201 gctgaccgct cttggcttca ccctcgtgcc gggcacccat ccgattattc cggtcatgct
     1261 gggggaggca acggtcgcaa cgcagatggc cgaccgcctg ctacaagaag gaatctacgt
     1321 ggtgggattc agctatccgg tcgtgccgaa aggacaggcc cgcattcgct tgcagctgtc
     1381 tgcagcccac acccgcctgc aacttgagcg ggccgtggcg gccttcgcga aggtaggccg
     1441 cgaactcaac gtgatcgggt aaccggttat tccctgatcg cgtttcgccg tttctgaatg
     1501 tacgcgttct tcactgccgt atacagatca agggtcgctt cctctacccc ctgaaacttc
     1561 tccagattca acgagcgatc gttcagaatt tcttcgacgc ggccgccgat ctgcgcgatg
     1621 gacgaggttg tccgattgtg atgggcgacc aatgaaggga tgccgttgac ctcgataatg
     1681 gggaacacca tccaattgat cggattcagg gcgatatctc caatataccc gacgagatcc
     1741 cgaagggtaa acggaggcag aaacggcaac accacgtagg gccccggctt catgccgtaa
     1801 taacccaatg tctgccccgt atcctcttcc ggcgtcgtga tgttcatata cttcgcgaca
     1861 tcgaccagcc cgcccaatcc gaaggtgctg ttcaggagga accggccgac ctcggtgccg
     1921 gctccggaga atttaccctg ggcgaggttg ttgatcagcc gggaaggaaa gcgggtgtta
     1981 taaaacaggt tgctgatgcc gatctgcacc gggttgggca ccaccgcgtt atacccctgc
     2041 gcagcgggtt taaggaccca gcggtccacc tggcgattaa actcgaagac cttcgtgttg
     2101 accggttccc aggggtcata ctcttctccg cccacgggct cgtcgctctt ggcaaacggg
     2161 tcgtaaaacg gatcttcggc cggttcgaca gtcggttccg tagccgcaga cggggcgctc
     2221 ttattcgccg ccaaaatcac attgggcgag acggctggag cggcagcgac ttgaactgtt
     2281 tccgccccct cgcctcgaga ctccgcccgt gcagcggact ccaccccgac acttgtattc
     2341 aacgtggttt gagacggccc gccggcacat cccaccaccg tcaagaccac gcctgcacac
     2401 accaccctca cgaaccttct cgcattcacc cctatccgat gcaccatgcc tcctcaccga
     2461 gagcccatca tgcccgattc gactgaaccg ccgcacctat ccagtgcggc aagcggacgc
     2521 actctagcag aatatacgga cagctgccag atgatgacga ggcctcatgc cgacctcacg
     2581 gccgagcacg cgatcgctcc atcgcatctt accaagcgag tccatgctga ccagaaaaaa
     2641 cgcagaaggt gcggagcgcc tctcaccacc tcagcgaccg agcgcagcgg caatgaaggc
     2701 ggacagtttg gcaaaatagg cccgcccgcc gaccgacggc acatcgttgt gatcggcccc
     2761 gcgcacgatg tagaactcct tcggcgcttt cgcggcgaca aaggcccgtt ggcctaattc
     2821 gatcgggata atgtcatcac gatctccatg cacaaacaac ttcggcaacg aaagctgcga
     2881 cagccgatcc tccaatcgaa acgcggctcc cagcaaccag tgcaccggca aacccatgta
     2941 atggtggcgg gcaaccgcct cgatcgatgg aaaacaggat tccagcagca gacccatggc
     3001 aggccgttgc gtcgccagtt ctccggccac cgcgccacct agcgaccggc caaagatcac
     3061 cagacgttcc ggcctgatcc gacggacacg cgtaagatag tcataggccc cgatcgcatc
     3121 ctgatagagt ccgtcttccg atggacgacc ctggctcttt ccataccccc gatagtcgaa
     3181 gagaaatacc gagagcccca gccgatacaa ggcacgaaga ttgtccagtc gatgggtcag
     3241 gtttccggca ttgccgtgac accagagcag caccgctgtg gtcgcagaac tctccgcata
     3301 ccacccgaac aacttcgtcc catcgccagc ctgaaaccag acatcttcca acggcacccc
     3361 gccgacggcg gaccaatcgc cctcctccca cggatgcgga tgatagacga agaattgatc
     3421 gagcagactc atggcatccc tgccggtcag aaaaacgcct gcaccgtaaa taatacgtcg
     3481 ttgtcgtgat cgcggtgatt gtattccatc cgtaacgcca aattttgagc gactgtataa
     3541 cgttgcgcca ccgaccaacg gacatcatcg gacttgtcgc ccaacgcata ccgcaaatag
     3601 gtcgtcgagg ctaacatccg ccaccgttca gtgagatcgg catagaagcc tgctgacgcg
     3661 ccgccgccga cgcgatgacg ctcttcatag gcccggctgt agttcccctc cacctcggca
     3721 aacacaaaca atacctcatg acgaagcaga tgtgtttcag tggccgcgcc gatgccaccg
     3781 ttcacatgcc aattgctgca cagctcgcag cccccatgtg tgatggtctg catccccata
     3841 ttgagcttcc aggaaggggc atggaacagg ctatccatcg gtgacaacga cagcacgttt
     3901 gccaacgtcg cccgttccac ccgcgtctga tccgcccgat tatagtggcg caggctgatc
     3961 gacccgagct cgatttgcgc ccccggtgta tagcccggtt cgggatcgag cagatcatgg
     4021 taggccgtcc gcaggctcac ttcttcatac gtgtcattat tgcgccagcc accacccaca
     4081 ctcacgcggg atgtcttatg gcccaattcc ggttgcatgg cgaaggggag aatcttgaga
     4141 tcttccgaag gaattcgcaa gagactccga gcggtcagga tctgccggtt acgttctcga
     4201 gagatcgcga cctgcgcctc gtcgcgttca cttatataca gagcatagtc ggacgcgata
     4261 tccaggacaa ccgcctgccg ggataatggc aaaccccgta catcctccga ttcaatgatc
     4321 gacccatcgc gcaccagtcg tttcaccaac ccctgttcca tgtccgacag gtgttcgcgt
     4381 ttgcgacgaa tgagcgtcac gcgggatgga cggaacgcga tctcgttcac caagcccggc
     4441 tcggccgcga gcacccggac cgtgtcggcg ggcacggtcc agaaacgaaa ccgctcggtc
     4501 agatgcaacg aagggtccgc atattccagc aacgacaaca gatggtacga gcaattttct
     4561 ttgaagaaaa aatagtcaaa cgaggcgtgt ctcagttccc aggcatgcat gagcagacgg
     4621 cggatttgct gttcattaaa attcaggcga tattcccaga tgtcgcggtt ttcgatatcc
     4681 cggtattcct gcaccttcag gtaatagggg acggtggaga actgcccccg gtagccgccg
     4741 aaaataccgc gcaccggata ggcgaggcct tcatccggag ggacatcggc tgcaaagttg
     4801 atcgtatagg ccagcagacg agtctgagcc gtttgtcctt cttgatcgat gcgcagaaag
     4861 gtatggccga acatggaggc tggattgttc atgaaggccg acggaaacac cagcgtcacg
     4921 gagcgggcat gaaactcatc gatccaacgt tcgaaccgtt cgcacggcat gggagggagc
     4981 tgactcggat caacattcag acgttccttg agccaggcgt accgcgcgac aaacgcgcat
     5041 tgagccggtt gcttggagcg accgaccagg tccggtgaaa aaaattgtct cagtgtcgca
     5101 tcgagttcaa cctcaggatt ggttttgccg tcgggagcga gaaagaatcc ggggtcgtcc
     5161 tgctcactcg tgtaaccgcc gaacagattg gcgcgatagt gcaggaggag atgccattcg
     5221 cgcgcatccg ccaatcgagc ctggcgggcc tgttgaagga gttgcgtcaa gtagggcgcg
     5281 ggcgcgtctt ccgccgcaac agaggtcgcc aggaaaagac cggccagcag gagagacagg
     5341 atgtgcacga tcgaacgcag agaagagaac aaggctccgg tcaatgaccg gagccctggc
     5401 ataattagcg gctgacgact tgggcgagga cagggctcct ggaaaccgcg tcattcaacg
     5461 tcttcaccaa ggccaccgac gaggtttcac ctgcctgaac caagtccgaa tagcgctcct
     5521 gggtcaacgc gaaaaacgcc ggatgctgtt catccggaat ccccatgagt gcagccagag
     5581 aggccaaatg ttcacccttg ccctgcgcca tctcctgcgt caaattctcg aagttcagga
     5641 gcgcaaacat ggtggtcttc tgatcagcca tgacctgccc atcgttcgta cagccggagg
     5701 tgcctgagct gattgcaaag gtgttcatga aggtcgcatt cgttagcgcc atgaatgcct
     5761 gcggtgcaat gttcttctga ttcttatagt ctgcccaagc caatttccca agaccgcagc
     5821 ctggtcccgt atcaggattg gccgccatga ccaatccggc ctgtgacacc atcgcgacgg
     5881 cgcccaacac gacgaatagt tttctcagca tatgtacacc tcctttgagt aagtgaatga
     5941 gtccttcacg aacggttctc cgtctgcagc ccgtcgatat tataaccaag ccattttgct
     6001 atgtagagca aaaaatccgg ctcgatcgcg cgagtgcatt actctgcgcg atcggccaag
     6061 gtgatgtgtt gcgcgtcgat cgcctctcca tccaacaaca aaagtccgat cgcgcgcggc
     6121 aggtgaaacc gcttcggccc ggcagaaccg ggattgaaca gcagaacacc gtcacgccat
     6181 tccgccttgg gctggtggct gtgcccgtac agacagattg caggattggt tcgcgatagg
     6241 aaatgggcgc cgtcgcgcgt cagctttccc ttttcgtaca atcggtgatg gagcgcaatg
     6301 cgatggcccg ccaactcaat cacctgctcg acggggaagc cgctcgcttc gaatccgtcg
     6361 acatttcctg atacgactgt gaccggagca atcgcttcaa gctgctcgat gacctctcgc
     6421 ctgccgatgt ctccggcatg caggatacga tcgactccgg caaagtggcg gacaatcgcc
     6481 cgatcgaata ctccatgcgt gtccgatacc accccgatgc gcatcagagg cttgcctcat
     6541 ttcgcggaat ccaccttcgc caatgagttc ccaagaacag cggtacgagt tcctgcaaat
     6601 acccagcaga actaacaagt atcttggaga cgaacgcgaa ccatgtctac cacgattagt
     6661 gtgctgaggg gcaggtgatg atcgttattg ttgccgggga aaagcacttg gccatgactt
     6721 cgcattttgg agggagagga aaaggctcaa tgtgcagtcg gattactggg cgccaatgcc
     6781 cgacattgtc gggcattggc gtggatacca attcaaatag gcactctgta cagcaatcct
     6841 tagtgaatac ctttcactgt gcctttgtaa cccgtcacct gaacttcgac attcttaaaa
     6901 ataaagaagt cctgtacatc cgtcacatac cttggagcaa tgatgatgtc tgctcccgat
     6961 ttgctaatag catcataggc tgcctgagat gccgcctcgt gagcgccaac agaaaataga
     7021 ctaaacaagc tcgagaaaaa atcacccggt tgaactgcat aaggattata cacacctagt
     7081 tgactgacca cggcaccatc atataacact ccgtctgcat acttgtttga gctgccaaat
     7141 gggatggtca ggaaatacgt aattttcgcg tgcccagata ttttttcccc caccgctaca
     7201 tcggctttta actcatttgc ggttagccgt acctgcatcg gagccgataa cgtattggaa
     7261 tgttgcgcaa tgcatccgct cgccaaagcg gcgactgaga acaacaaggg agcaatgagt
     7321 ctcagcttct tcatgggaac tccttttgtg agagttaagg gaagaaaaac caggctgctt
     7381 atacaacaca gcttataaat tggcaacaac agaatactta tggtttcctg ttagagacgg
     7441 agccacagca tggtgatgca agtatggcaa agcccgcaca aggacgagtg atcgcctgat
     7501 cgaacaatcc gtgcgtatcc gacatcaccc caatgcgcat cagaagctcc ggccctattt
     7561 cctttgctcg gatgaactca gcgaatcctg cacctgcttc cactcgaagg tgaacggctc
     7621 aggcgtctgt ttgggtaacg cggccagttc tgttttcaat cgttccgcac gggccgcgct
     7681 catgggatgc gtggagaaga gctccacacg ttccttgtct ttctccgaga gacgttcaaa
     7741 aaaccggatc atgccgtccg gggcgattcg tgcatcggac aagaggcgaa tgccggtgac
     7801 atccgcttcc gtctcctgct cacggccgaa cttcaatgtg gccaactcca agcccagttg
     7861 cctggcaagc ccgatcaggc cttgctgatc tcccacgaga atactcacca ccgcggctac
     7921 ccccatcatc ttcacgaggc gttccatccc atgccgctgc aacacgtggt tcaactcatg
     7981 actcaacacc ccggccactt cttcgccgct ctctgccttc ctcatcagcc cggtgaacac
     8041 cacgacgtag cctcctggca gcgcaaacgc gttcaccatc gggctctgga ccaccgacac
     8101 ctggaacgtg taggggttgt tgggaatctt ggccgtcatg cgctgagtca tttcctgaac
     8161 cgcgctgaca gccgggccat ccttgagcac cgtttctttg gacaagaaat cctggtaaac
     8221 cgtttcgccg agcttctgtt cccactgaac cggtatgcgg gcgaccgccc attcgacgat
     8281 cagatccgac ccgaaccaca ccatcaaccc cagtccgacc accaccccca cggcgctgcc
     8341 ccacaagaca cgatggctgt ggcgcgcgcg acgaacctgc tcggctgccc gctcaagatg
     8401 cgccatcaac gcagagggag cggcacgacg aaacgcgatg atcaatgccg gatccttgag
     8461 atacagcgtc cggaccgatg cgccgactcc ccacgacacc accagctgat catgatccag
     8521 gcccccggcc tcaacggaca tggccgtgaa cgcgatggcc tcttcgagga gcggagagtc
     8581 tccgacagaa gccatggtca ggccatccgc cgacaccgta acatagcaag gcgcacccgc
     8641 tcccggcagg ccaccaccga aacagagcgc gttgggagta gggttcatcg ggttcgatct
     8701 tgcggctgat caaaatggtt gtgaacgagg ccacaggcga ccgcaaaccg gaggcgtacc
     8761 ctcagcggta cgttgaggat ttgtcgtgag ccgagaacga agtgcacggg cattttcatc
     8821 agccgctact cagagaccgg gatgaattgt ctgatccaat tcccgaagct tccgggattg
     8881 cggctttgaa tgtagaccac gccgggacca cggaaccggc acaccagtcc ttcacccgac
     8941 gtcaggctgg caatccagcc ggaggaggcc ttttcgatat tgtacgtggt ggtggaagtc
     9001 caggcgacaa gatggctgtt atcgacgatg tactcttcgt tcggcttcag ctcgattttg
     9061 tggatcgccc cgaagctgtt caggatcagc tgaccggtcc cgccgatctt caggataaaa
     9121 aacccttccc cgcccagcaa cccgcgcgtg aggctctgca tcttgctttc gatcttcaca
     9181 ccctctgcgc cggccagaaa cccatccttc tgcaccatgt attcattcac gccgtcgagg
     9241 tccagcaaca caatttcgcc gggtaccgtc ggagccaaga gcacttcccc cgcgccacga
     9301 ctggcgcgca gcgtctggaa gaaaaatttc tcgccgctca gcatcttccg cgataacgcg
     9361 cccagaaaac cgccttccat cttgctttcg atatcgatcg tcggcgaggc cgctaccatg
     9421 gcgccggatt cggccttcac cgtctcgcct tcgttcagat acacacgcac catcggaaaa
     9481 gccccgggat acaaaatctc gctcttcatc tcgcactccc ttcttgaaac cacagcctct
     9541 gacaaatcac ccgattctgt ggaatgtccc ccatcctgtc aagtcacgcg gcacttccag
     9601 tgagccgccg gtcacccagc cccttcggcc ccctcaagat cggtggagtt tactccgata
     9661 gagaccacac ggacgccctc atgtccggag gggcgaatcg tccccacagg gaatgccatt
     9721 gtgactgacc tcccaggtga acgtatcgaa cacacgcgag cgcacccatg aagcgcgtcg
     9781 tcatcgaaga actcacaacg ggcatgattc tcgccaaacc tgtcatcaac aatgcagggt
     9841 tggtggtgct tcctgtcggc gccgagttgg atgaagcgac cctctcccga ttgcagcgcc
     9901 tcggcctgac gtccgtctat gtggaggggg aggcgggtga cgcgagcggc aagacgctcg
     9961 acgagctgga agccgagctc gaccaccgct ttcgactggt ggcccaggat ccgattcaat
    10021 gtctactgcg cgaggcgatt cgtctccacc ttcgaaccac gcatggaatc agcgacaatc
    10081 cggaggcgac gccatcggca tgaatccttc aaggaaaatt gatctccacc gacgaatcga
    10141 gcaagtcggt gagttaccga ccctcccgca tgtcgtccag aaactggcct ccatgatcgg
    10201 tcgccccaac gtatcggcgg aagagatcgg aaccctactc gaaaaagacc aagtgctgtc
    10261 cgccaaagtt ttgcggcttg ccaattcgcc attttatgga tttccttcgc gtattgcgtc
    10321 ggtcgcccac gccgtagtgg tcctgggctt gaacgtggtg aagggcctca cgctttgcgc
    10381 gactgccttc gacatgatgc gaaacgccgg catgaacgaa ctctggcgac actcactggg
    10441 agtcgcgatg accgcccaca ttctgggcac caaggcggcc atgaaaaatc ccgaggaagt
    10501 ctttgtcgcc gggctcctgc acgacatcgg caaggtcgtg ctgtatgtca aatggcccga
    10561 tgtcggccag cagatcacaa ccgtcacccg caacacgtcc cggcccctca tggacacgga
    10621 gcaggaattg ttcgaggtga cccatgccga tgtcggaggc tggctcgcga gcgcctggca
    10681 tcttccgacc agcctgcgtg aacccattct ccatcaccac acccctgcgg ctgctcagga
    10741 tgcgaagctc caaactgcca tcgtgcatgt cgccgacgtg ctggtgaagg gcctggcctg
    10801 tggcaatccc ggcgatgacc tggtccctcc gctgtcccgg caagcctggg atctggtggg
    10861 cctggatgcg cagagcctcg cccactgcct tacccaggct acggaagagt tccagaccat
    10921 cgacgactac ctatgagtgg atctccacag aaccggcgca ttctgttgct gcacagcgac
    10981 ccgcttgaga tcgaacggtt aacggccggg ctgaaccggt ctggatttac ggtggtggca
    11041 gcggatgcgg gccgcctggc gatgcacgaa ctggtgcatg atccgccctg tctcgtgctc
    11101 gccgccgaag gcaccaacgg ccgctcggca gacacgttag cccgggactt gcgcgccgac
    11161 cccttcctgg gacggttgcc cctgatcatc ctggtgcgtg atatccgggt caacgatttg
    11221 gattgggcga cgctcggggt ggacgactat atcgccgtgc cctaccgtcc cgacgaagta
    11281 ccgcaacggg tgcgcctctg cctgagccgg cttgaacgct cactggacgc caatcccctc
    11341 accagactcc ccggcaacag cacgattctc catgaaacga ccgcgcgcat cgaaagccgg
    11401 gcacccttcg ccttggccta tctggatatc gacaacttca aatcgttcaa cgaccggtat
    11461 ggctatgccc gcggcgacga agtcctggta gtcacctgcc gaatccttac gaccgtggtc
    11521 agcgaactgg ccggtaccga cggattcgtc gggcatgtgg gtggagacga ctttgtgttc
    11581 atgagcgctc cctccgccat cgatgcgatt tgccagactc tcatcaagcg gttcgacctg
    11641 gtcattccgg atttctacga tccggaagat cgacaggcag ggttcatcga ctccgtcgac
    11701 cgccgcggca accacgaacg atttcccatt atgagtttgt ccatcgccgt ggtgacgaac
    11761 gaacatcgcg acatctccca cccgggagat gtcagtaaga ttgcctcgga attgaagaag
    11821 gtcgccaaga gccggcccgg cagcgtctat gtgaaggaca accggacaaa tcccccggat
    11881 tcagcccctg aatctactcc ccgctaggtc ctcatgaaga gcgtcccccg agggagtccg
    11941 agcgaaacac ctcaaccgtt acggcgccga cgatccgccg ggggcctgcc caccgacatt
    12001 cccgccatac tgacgacgaa gttcacgccg gtacgcgcgc cgttcctccg gtgacatgtg
    12061 tttgaatttt tcccgcagtt ctttccgctg ttcgggtggg agggacctga accaccgccg
    12121 ggcatcgcgc accgactcgc gttgctcagg aggaagctgg cggaactgct gataccgctc
    12181 gcgtagctgt tgctgctgct ccggcggaag cgtccgccat tgtttgaacc gttgcttggc
    12241 ttcctgacgc tcttccggcg tcatcgtccc ccactgctgc gccccgttac gcaaccgttg
    12301 ttgtttacgc gggggcagct gatcccagtt gtcactgaac cgttgcagca gttgctgctc
    12361 gccggggctc aactggctcc acggcacgcc cgccgaatcg ccctgcgccc cggcagaggc
    12421 cacgccgcat gccagcacga ttacacacac cagcaacgct ctcatggtcc ggtctgcatc
    12481 ctcatcggat caggtggcac gttcgaatta cgcttctgct cgccgccctt cgacggtctt
    12541 cgaggacttg tcgctgggtc tctcgtatcg tccttcgaag tcggacgttc gtctctggga
    12601 agatcctcac caatttggaa tggatccacc cagcggccgg agtcgttctg ccaggatccc
    12661 agaaagtcga gaacatcgac atcgacctct tccggtcgcg gcgccggctc cggagctgcc
    12721 tgggccagtg aggcacacag atacagaccg agccacagcc aaggactagc cggtcgtggc
    12781 ggtgccatcg gccagccagt tgtaaaattc gagatcttcc gacaattcga tattttcagg
    12841 ggattgcatc agttcgagat cttcgagcag gaggtgggag tgattcgctc cgttggattg
    12901 ccacagccag atggtgacca tcagcgtggc catcgatgcc agcgccacac ccccggccca
    12961 ccgaagccaa ggttgccggc gccgcgaagc cgcgtccagc gcagccagtc tggcccgctg
    13021 caccgacagc actgtcttct gatcgagatc ctgcacactg cgatcgagga ggtgtttcgc
    13081 cgcaaccgcg agcggatccc gttgttcatc cgatcgttcg ttcatggcca atgcgctccc
    13141 aatcgcgttc gcaacacctg caacgcccgg aacaaatgcg ttttgacact gccttgcgaa
    13201 cagcccatag cttgagccgt ttgtgcgaca tccagttctt cccaaatgcg taacagaaac
    13261 gcctgctgct gtcgcagcgg aagggctcgc agcgccacat ccagctccgc acaagcacgt
    13321 ttacgctgca cgtcctcatc cggcgccacg gcggtcgaat cggcaatttg ctccagggga
    13381 tcctccccct cctcctcatc gtgagggctg cggaaaaatt cccgcaggcg gttgcgcacc
    13441 ctctcgcggc ggtaccagtc gcggatgcgg ctctgcagaa tacagtggaa cagcgggccc
    13501 cactcctcct cggcccgttc gccgtacttg tgcgccagct tcaacatggc atcctgcacc
    13561 agatcgagcg cctcatcctt attccccgtg gcaatatgtg ccatgcggaa tgcgcgccgc
    13621 tcaatccctg ccaggaaccg atccaatgcc tgggttcgat ccagtgatcc caccctctca
    13681 cccaggcaca gactgcctgg ctgtccatcg acggcaatct gggaatgaca gccgttcatt
    13741 cccgcctatc cacttccacg cggcaacctg ccagaccgca atctccaata cctgccgaca
    13801 cgaggaccgc ctgctgcggg caagtcccat attgagcact tctacgaacg cagatacggg
    13861 tggccccggg gaatctgagg tcggttcgat tctcgacccg aacgaccgtg aaatcaagga
    13921 aaggcaggca atgcaccatg gcatcgccga ccatccgctc gtccatcccc tcttcccgtc
    13981 tctggatagt cacaccctct cccctagacc gcgtgctagg agaggacaac gcaccaggat
    14041 gcttccggtt gaccgggctg aaagggaggg ttgagggagg gtcaccagca gagcagaatg
    14101 tggttgaggg gaccgggtta cttggattta tcggcgtctg caggccgctt cccacccgcc
    14161 catgcgacgg agggaattgg cgccaccagg tcgaaacggt cctgcgcttc attgtccttc
    14221 aagtcctggc cgacactata gagaacgccc ttcttcatat tcaccagcat ggggaaaccg
    14281 gtgaaggggt cgtagacgcc ttgtcccgcc ttggcaattc tggtgagcag gtcctgttcc
    14341 ggtggtgtcc ggcgtaacca gccctgcagg gatgccagtc gcaagcgcgc atccgtttcc
    14401 agcacacgac ccgcgtaggt ctcccatgtc ggaaggggat ccaccccgac gaggctttca
    14461 atgggattca tcacgatgtc cccgagccca tacgggggcg cgtgcacgaa ctgagactgt
    14521 ttcggcaaat cggtgtaccg cccctctgca gcagccttgc ccgcggcttc ataatattgc
    14581 gcataggcgt tgaaccggcg ctgcttcggc aacggcaacg cggccgcaac ggggccgtag
    14641 aacggtcggg catcggaccg gtcctcgctc accgcttcgt ccagcgtctt ggtggccagc
    14701 acgaattgac tctgcatcgg ccaacggacc gactgctctg cctgttcaag cggccgggcg
    14761 agtttggcca atcgactgac gaatcggtcg tcgagttcag gccggagtaa gagtccgctc
    14821 atgaccacaa tgtcatcatt catcgcagca ctggccagca tttttaccgg cagcgtcttg
    14881 gcctgcccca acaccgtccg ccaggccgta agatcgctct caagccgtgt gactccggct
    14941 tcgacatcct gtgcaaaccc ctcggcgata tagagtcgat gggcatagag aatcagacca
    15001 cagttcgggg tgatcgattg tccatagccc cagtcttcaa agggcttcgt caaccactgc
    15061 cggtatcggc ccatgctgac ctcagcccgg gatgcccatc ccttcacacc aatcgcgtcc
    15121 gcgcgcatct gcgccgccgg atcggcagtc ttcatccact tgcccaccga ctccgccgaa
    15181 accccctgat ccccgccgga gaatactccc tcaccggtca ggcaagtgtg ggcataggca
    15241 cgatccgccc cttcaacgcg ccggtccatc ccggcctgca caggatcctg agacgccgca
    15301 gcaccgaatc caagtaggag gacatatccg ttcctgctgg aatcctccaa tgtacgttgc
    15361 ggcaccgacg tcatggtgcg ataggccgca ctgggctgct cctccaggac cagccatgtc
    15421 agtgcgacca caccgaccgc tccgccgatc agtaccagcg tgagcccgac cagcgccagg
    15481 accatgacga ctaacgattg ggtcgtcgac accccggctc caaccagaga ggccaccagc
    15541 tcgcccattc gagtccacgc cccggctccg acaggctgac tcggcttggg gcgagcaaca
    15601 ggcgcgtcta caggcatagg cgccacagtt tcctgttcgt cgggcgggcg ttccaccgcc
    15661 acaacggcgg gttcctgatg ctcgacagac aaggctggaa caggagccgg cgcttcatcg
    15721 gaaacggtcg ctgggggagt ggcttcagca acttcagccg gaacgggttc tgcgcgcttc
    15781 ttgagccagc gtgtcagcat gttcctggtt gaggcgctcg gagtcggctt agacgacgtg
    15841 tcatcccaca gaatccgcag tccctcaccc gcgtcgggcg gagaagccac ctgagccatt
    15901 ggagccggcg cctgctcctc cgaaggagcc acagactcag caggtgtctc cacgagttct
    15961 ggcgatggag agacgaccgc cgcttgcgtt ggctcgaccg gaggcaaaac cggttcctca
    16021 ggtgcgacaa cgatcggcag aggaccccgc ggaagtgaga ctgcttcagg aggcggcgcg
    16081 acctggttga gcgatgcctc caccggggga acaatgaatt cgcttatggg aaccgtgtca
    16141 acttcacgaa cttcagccgc tgatggagag gatggctcct gatcggtcaa cgccatctcc
    16201 ggctctgctt gaggtgtcac ctccagaaca accgacgaca agggggcatc cggtgtaacc
    16261 ggctcggctc ccgcaactgg cttctcgtcg gctaggaaag gcgtggcggc aggaacacgg
    16321 ctctccgcga ccgcttccgg tgcaagctcg actatgacct cctgtgcgta aagaatctcc
    16381 gcgggctgct ccaccagtag gactggctcc aacggcagtt ccgcaactgg ctcgccgacc
    16441 ggtacctgag gctcttccac ggtgacctgc tccggttcca tcgacgtcac gtcggtgctg
    16501 acggcaatgg caaactccgt caccggttcg actgaagcag caggcggttc ccctgaactt
    16561 gtggcgacag ccgactcact gtcggccaga cgcaacggag gttccaccgc tcgatcgatc
    16621 gtggattcgg ccggttgttc agcttcaggc acggcgtccg gagtcggcac ctcgaccgcc
    16681 ttgtagggct cgactggaat acgaatagaa tcctcaggcg cctcggtcgg agcatccggg
    16741 gagagaatcc gcaattcagc ggtcactgtg gcaataggct ccaacggccg atcagcgacg
    16801 gtctccgcca aacaagcctc tctagcaacc gttgcctcag tcacgtctgg cgcagcaggc
    16861 aatgccaccg tggagtcctg taccgacacg gcaccctcgg caaaggccgg gccaaattct
    16921 ggctctggct cttggcgcgg aatggtgaca tcgtccactt caatctgttc ccatggcatg
    16981 ggcgccgaca aggttgtggc tgtaccggcc tgatcagccg ggatgtccgg gaccgtcgcc
    17041 tcgaacaagg gtgactcttc ctctggcgaa gctggcggag ccgtgagagc gccagccgtc
    17101 tgctcctgta ccgcggcatc gaccgcaggc ttctgctcac catccgaaag cgtctgagcg
    17161 ggcgcaggag acgtttgcat ggccgccacg gccgcaagaa tttcctccca tgagagtccc
    17221 gatgaggtga gttccgaagg acgcgatgaa ggaaccgact ccgcggcctg actgacctcg
    17281 accgcatggt ttggcgcagg agactcgatc gccttctcaa cctcgtccgt tccaggctcc
    17341 accagggtct cagtgcgcgg atccacagca tccgttgctt cttcgatctg atcccagggc
    17401 atcgacgcag gacttgtcgg cgcttgggca ataggttggc tactggcctg cgtgaggact
    17461 tcgatcgtcg gcgcgatggg ctcagacact ggtgcctgga ggtccatctc ttcgaacggc
    17521 atcagagcgg cagcagccgg cggttcaggt tcgacaggtt ggaatggagc caccccggcc
    17581 aacggcagcg ttgagacctc attctgcgtt ggcggcaatg actcgggttc aaccatgacc
    17641 ggttccggac tcgacggcgg cgaggcgatt acgaccgcaa cctcttcgat ctggtcccaa
    17701 ggcatgggcg cggggctcgc cggtgctgtg gcaatcgggt gcgcactgga ctgcgtgagg
    17761 acctcgatcg tggggcctgc caactcggcc actgatggtg tgggagccgt ctctcccgaa
    17821 tgggtaggag caatacccgc cggcgacgcc gccggtcctt caggctgcga aggaatgatc
    17881 ccagcggcct cttctacttg gtcccaaggc atcggagcgg gctgtgccaa cggtgtagcg
    17941 atcgagtcga gaccggtttt caccgacgcg tcaatcgcag ggagggacac ctcagcaacc
    18001 gatgcaggca ctagttcggc cggctctgtc gaaacaagag cgacatctaa gacaggaaga
    18061 tcactttcac cggcaacctg caccggctcc accgccgagg tgacggccga aactgaagac
    18121 tcagattgaa gttgaacctc cgactcctgt ggcgaatcga ccggcagcgt aaccgacagt
    18181 ggttcctctg gcctagcctg cgacagacct tccgtcaccg cctccagcct cgagggggtg
    18241 gtggtgaaag cgatcacctc aggggactcg accgccgaga cctgaatctc ctgtgggcgt
    18301 gggacgtcac tggaggccgt ctccacttgc tcccaaggca tgaccaccgc agagtctgta
    18361 ccctgggaag tctcggtcag taccggggcg tgagcggtct cactgataga atcgaccaca
    18421 ggaggctggg gagtaacttg caggacctgc ccggttaccg tgtcgaacat ggccgcaaaa
    18481 cgaaaggcca ccggacttcc cggggctagg gaacgaatcc gcgcaaagag ttcgctggcc
    18541 cggttcggat ggtcggtgtc cggatcttcc agcaacacct ccaccgcctt gccgtactca
    18601 gccaccgccg ccatgacgtc gcccttttcc tcatacacac ggcccagcat ctccaggaac
    18661 ggaacatagc ggggtcccgc cgtcagatat tcccgcaaga gcgactccgc caaccaatag
    18721 tcctgccgct ccatcgcttc ccgagccagc gattcgaacg ccgtcctggc gctcatgaga
    18781 ctgccgagcc gcagatggag agtggcgaag aggcgccggg cttccgcgtt gagcgggtca
    18841 agcgcgagca ggtctttcaa cgcggaggac gcccggccgt acttgccggc gttcatttgt
    18901 gtaatggcat cttcgagaag cgccgacttt ttgttaaagc cgatgctccc ctttttctgg
    18961 accttcggag ggactttttt gtctgacttg agaggagatt tcttatcaga tcgcactgta
    19021 taaacctata aagttacaga catttctcgc gccgcatgac tccatgacta gaatgccagc
    19081 agggactatc ccaaccgggc aatccaccgc cgagaacaac tgcaccattc gggggaaagc
    19141 aagggaagtg ccttggccac tacatggtcc tcttctgagc catgatgcgg tgatgaccgt
    19201 ggaatgcctg ccgtttacct ggataacgtc acccagctct ccgacacagc catgtgtcta
    19261 tccttttcgg cattaccggg cacgatcttg attagggatt ctcctgtgcc ggttctgaca
    19321 cacagtctgg aagtcgccac cgtcggcaga cgggagcact gactgtccga gaatgatgtg
    19381 ttatccgtct ccgggggttc gtatactgtg atcaaggagg actccttgac caaggcccac
    19441 atcctcatcc ttacggacct cgacggaacc ctgctcgacg ccaccaccta ttcctacgag
    19501 gcagccacgg gcgccctctc tgccattcag accctgggtg caacgcttat cctggtttcg
    19561 agcaagaccc gatcggaaat ggagccgctg cgacttcgcc tggacaatcg ccatcccttt
    19621 atcgtcgaaa acggcggagc gttgttcatc cccatgggct atttcccatt tcctttggag
    19681 caggccgcac cccgcgggga ctacgaggtg gtcgaaatcg gcacccccta tgtccggctc
    19741 aggacggccc tgaaagaaat cgggcgggac ctgggctgtc gcttgcgagg gttcggggat
    19801 ctctctctcg aagaggtgtc agacctcacg gggctgtctc ctgcggagac tctgctggcc
    19861 acccaacggg aatacgatga gccggttgtt gtggaatcga atgggatggc ctggaaccgc
    19921 ttgtcagcag cggcggcaac acgcggcttg cgctggaccc gaggaggccg gttctatcat
    19981 ttgatgggag gaaacgataa aggggttgcg agccggcgac tcattgcctg gtacgagcgg
    20041 cgcgcacagc aggaaggacg cgccctgatc accgtgggca ttggggatag tctgaacgac
    20101 cttcccatgc tggaagcagt gacctacccc atcctcgtcc ggaagccgga cggttcctac
    20161 gattcggacg tgcagtttcc ccacctcacc cgcgccgaag gggtggggcc tgtcggatgg
    20221 aaccgaagcc tcatggatct gttgccgacc ctttgacgat cagcggatcg gcactcccga
    20281 accacccggc accaacgctg caatggtcga ctcatggtta ctccttcgca gagacaggag
    20341 gacgacgccg acactggaga cctgccagaa gcccaccggc aaaccctagt ccgaccgcaa
    20401 acgccacaag gcccacaagc gagcggttga acacgaacgg gctctggcgc cggtgcgggt
    20461 aacggggcac ggccgcttcg ccgcccagcc agcgccgcgc cttggcctca gcatcgacat
    20521 ctgaaatttt atcggtcacg ctagatcctt accagctata tgttaggtgt cgaaacagcg
    20581 gttgcctaga gatggcgcaa gtcatcaggc gccccgtccc gcgtgcggat cgaaagcccg
    20641 cgctcaagat agtctttggc caaatccttg gcgcccaggc cgaatgcaat cgccccggcc
    20701 atgacgagcc caccgagtgt aatgccgaat cccaccacca cgatattctg ggcgatcccg
    20761 agttgctcaa gcgccatggc gaccgccaac agttgaatcc cccatcgcga gcaggtcgcc
    20821 accagtcgcg ccggaggcaa ccctgcattg acggcggcga tcagaaccgc ctgggacaca
    20881 aagttggata ataaccaacc ggcgacgaga atgagacccg ccgtcacgag atgcggcaca
    20941 tacgccagga acgacttggc gaaatcgttg atcggctgga gattgagcgc acccaaggcg
    21001 gcgacggtcg cgaaaatcat aaccaaccaa taggccgctt gccccaccag acgtgacgga
    21061 tcggttttga cccctccgcg cagcaatgcg cccgtcactc ccagtcgatc gaacaatcga
    21121 tccagcccga ccacccgcag caaccgctcc agcgcctgac tggcggtcca cgcgaccacc
    21181 aatcccgcga agaaaatgat cgacatcgcc aacaggctcg gtaacaccgc aagcacctgc
    21241 cgcgccatca actccagggg atcgagtagc cccttctcaa aaaattcttt catacttccc
    21301 tcctccggcc tctactgcag cctgccttca gatggatcga gttgcgcgca ttaccctgca
    21361 tgtggctcat tccagcgcgc caccagatag gacttatcct tttcaaacgc ctggcacaag
    21421 gcttcgatgc ggccctcggc ctcgctcgat gtgagcccct gggtatccaa cacaaacgaa
    21481 gcggttcgcc ccaggtacag gggagtgaag gccttcaaga catggtcgcg cggcaacacg
    21541 cgattgcggt aggccacggc ggcgtcatac acgacccgtg cccacagatt gtcggcaata
    21601 cgaaagtctt gcgccgaaca ggttcccaac ggcctcagac tctgtctggt gccgggagcg
    21661 agaatctgat cccaaatcgt ccccagatca ctcaggccct gtcgaaaggt gctgatcatc
    21721 cgatcgacat tgacgttgac cggctcgacc ccgacctcgt attgaaaccc gaacaacggt
    21781 acggcattcg agccatccgt cttgccccac accgcatagt gatcttccat cagcgcgaac
    21841 acggcgccca gcacctgcac caacatcgcc gacaggtccg ccgccggatc tttggggttg
    21901 tggatcttcg cgcccagaaa gctttgacag acgcgcgctc cgctggcgat cgcttccgtc
    21961 gtcatccaga tgtcgatgcc gaacttggcc acttccgatt cccacacatg cttgtcgaga
    22021 tagtgctggg ccaactggcc ggaaaaaccg aaatcgccgc cgatgggctg tctgatacga
    22081 tgaccataga gggttcgcgt cagcggatac acaatgctgt tggtgatagt cccgtcatac
    22141 ttgtgccgca gataataggg cgcgacgtag tcgaacccct gctcaaacac cgggctcacc
    22201 agcaactcaa tccattccgg ggtgatgctc cgcagatccg agtccacgac ggcacaggcc
    22261 ttggctttca agcgacgcgc aatttcaaaa atcgtgcgaa aggcgctccc cttaccggga
    22321 atgccgtgat acggcgtgat gatcttgtgg agcgagctct gctgatcgcc gatgaagagg
    22381 gtcttgagat cgacgacggc ctgcgaaacc acctgctgcg tcccgtcggt cgagccgccg
    22441 tccgaattca ccaatacggc gcgggcttcc ggaaaatact tggccaggcc ggcgctcacc
    22501 gccttgacga catgcccgat ggtttcgaca ttgttgaagc tgggaatccc gaccaaaata
    22561 tccgtatggg cgaattggtc gacctgcgcc tccgtttcgg gcgtcagtgg ggtggtatgg
    22621 gtcgcatcgg gaagttgggg gccaggcgtc atacgtcaag tctcgcacgc ctccgccctg
    22681 gcaaaccatc tatgacatga ccgagctgga ccggcgaaag gctggtgtgt gtaacaacgt
    22741 gtcttcgcta agagacacca tcttgcccga tgatgtcaag caatcctgcg gccacgccgc
    22801 ttgaccacct tccgcatctc gctcacacat ttcaccaaca cagcagcccg ataggcggca
    22861 gacctcgatg acacaaggcg catgtacgca gaatcacgag ccggcaggta aggaaaaaga
    22921 gcgaggacaa gcggaggaac attcgaatcg gctatccggc gtcggaaatg acgatctgtt
    22981 ctttccgatc cgtactgaac ggaggagtgg tgtcatgggc gctgacgaag tcgttcaaac
    23041 ggagttggtc atcggcatcc ataagaatgg gatcgacccc cagcttcgac tcgctggccc
    23101 agcggatcgt cgccagcctc accgagaggg gcctgggctg ccccggaata tgcaaatgca
    23161 gttggagtaa gtctcccgct tgcaatcctt tgagtccagt ccgtgaccat cccggaatcg
    23221 agagatcgag catccggccg ttccacacca acaaaccatc ttcggagcgt gatccggtac
    23281 actcgacgat aaaacgcggg cgattctgtt gttccatcca ttcctccaag caaacgcctc
    23341 ggcagaatat gaaaaacaac gaatagcctg atagtccccg aaaggtgggg gcgctccggc
    23401 ccgatcgttt ttatcctgct cagagaggtt cgtagtatcg ccggcgaaac ggaaggagga
    23461 gggaacggaa acgagagctg ttgctgcgaa ccggacccag actaggtcgg agagccgaat
    23521 aaccgataaa cgattaagga gtcacgcaac tcgtgaagat cccgactcct tgagcactga
    23581 ctccacaaat ttgtgaatgc gtgcttgggc ggtagcgctc atcctgacgg tttcaagccc
    23641 aaagacacgg ccttgcaccc actgaaccgt caccccatct acatcaatcg ccttcggctg
    23701 gtcaggcaaa aagacgcgca acgtgagttt catgccgacc gtgaccggct tatccccgtc
    23761 gatccgccag cccttgaccg aaagatcgca caccttgccg gttccgacca ggccctcccc
    23821 gagataatag aggaagcagc tgacgggcac acgggaatgg gaacgagatg tctgtgactt
    23881 agccatgatt tccggcgagg gagagggctc gatgccgtct cagcctcgcg acgactcagt
    23941 ctactcaact cgctggggat ggccaagaat attcagtttc tcgtcagggc aggcagtccc
    24001 gcttcgcaat ctcatagatc ggaaaaagat aaatggagaa gggcagcggt cagactaagg
    24061 ccgtgacgaa ctgttcgagg cgagcatgct catcggcctt gatctcattc acctcaatcc
    24121 cgaattcctg cccacgcgac caccgcacga ctgcgcggtc tataaacacg gtgggatgct
    24181 ggtcgggaag aaacacgcag agggtcacga ccgttccggg ctcgaccgga tgtgtcccgt
    24241 cgatacgcca cccattgagg gagaggttcc aggcgacgcc ggttccctgg agcgcatcgc
    24301 tggagtaata cacagaacac tgaacgggaa aacgacgaaa ggaccgcaac tgaaatgtcg
    24361 tggcgaccgg agccggcgag gagcgcgggg ccaatggagc gacctgtttg ttgccaccgg
    24421 tcatttggct acccgctcga gtgatccgta tccgcagacg ttggggtgag tgacgggttt
    24481 cgaacccgcg acctccggat ccacaatccg gcgctctaac cagctgagct acacccacca
    24541 caccaggtca acgaggaacg cgcatcttaa caaaccccga agaacagtcg caaccgcccc
    24601 gagacctcac cctgactcag cttttgctgc gtagaatggc cgggcgaagt cgcgacgcat
    24661 tctgtatccc cggtcgcatg gacgaaatca acttgggact tgaccgtcaa cgcccctggc
    24721 gatcgaaggc cgcttgcatt tcttccagga tggccgccgt ggcggcagga ggatcctgag
    24781 catcgcgaat aggacgaccg atcaccagat agtcagcccc ggctgcaatg gtctgggtcg
    24841 gcgtcgtcac ccgcgcatga tcatccgtcc cttttcctgc cggcctgacc ccgggggtca
    24901 cgataaccag attcggtccc accttggcac gaatcgcctg cggctcttct cccgacgcca
    24961 ccacgccgtc gcagcccact tcggccgcga gcatggcccg cgccgttacc aaatcctgca
    25021 cgcttcgctg aatgcccatc tcccgcaaat cttccccgtc gaaattggtc agcaccgtca
    25081 ccgccagcag tttgagatcg gacgatcccc gtccctgcac cgccgcaatc aacgcttttc
    25141 gattcgcatg gacggtcaga aaggtggcac ccatagcagc gacccgagcc gtcgcacgcc
    25201 ggacggtttc ttcaatatcg aggaacttga gatcaaggaa cacccgcttc ccacggtcca
    25261 ccagcttccg gacgatgtcc ggccccgcca ccgtgtacaa ctccaggccg actttcacaa
    25321 atcgcacctg gtctcccatg cgagccacca gcgcatccgc ttccgccgcg gagggaacat
    25381 ccagcgcgac aatcaaccga tctcgtgcat caatccgtgt catcatgtgt acgccgttac
    25441 cccctggttg cctcggggcc cggctgactc ctttcagtgt ccttgacctc tcttctcatc
    25501 ctatgttaca agctcaagct ttgaacgtag gagaatccgt atgcctgttg tccacaaatc
    25561 cacgattcgc cgtgcccgtc aatctgagaa gcgtcgtttg cgcaaccgct ccaccatcag
    25621 ctcggtccgg agcatcctcc gcaaggttca ggatgcgatc gcggccaaga agccggatga
    25681 agccaaggcc accctgcggg aagccacttc agcgttgagc aaagcggtca ccaaaggtgt
    25741 cttgaagccc aacacggcct cgcgccgagt ctcccggctc accgtgcgtg tgaacgcaat
    25801 caccgcttcc gcgtaacggt tctgacattc gacaccggtc tcgaacccga cgggcgcggg
    25861 accggtggtg ccggctgcac ccgcgctgcg gatgatgctg atttcgacgg ctggccacag
    25921 agtcggaaca agaggtcttc catcacccga acaggggcgc ttccacttcc gcctttcagc
    25981 ttcgagtcgg tttccaaaaa ccagcgaaac gtgtgcgtca gttgcacatc tgagaattgc
    26041 gccagaaacc cgcgcaccct ggatgggtcc atccgtaacg tcctggccgc ttccccttct
    26101 cgaccgccgg ctttgagctg ctctttcatt ttccacagcc gtcggtattg ccagaccaag
    26161 gcaccgagaa tccggagcgg agcctctcca ttctcaagat ttcgcgcaag gatccgtaac
    26221 gcccgtccat gatcgtgcgc accgatggcg ttcatgagat cgaagacgga tgcaccggac
    26281 tccgtccctc tcaacgcctc gacatccgcc ggttgcacgg ttcggtctgg cggcacatag
    26341 gcggcgagct tctctaactc ccgcttcacc gcgtagagcg actcgctccc cacctccttg
    26401 agcaattgcg tcgcctcctc gtggatgcgc acacccagcg ccgccgcttc ctggcgaatc
    26461 cacgccgaca gctgcgcatc gcgcagcggg gcgcaattga ccgtcactgc ccctttcgtc
    26521 aacgcttggg tccatttcat ccgtccatcc agcttgacac ccaccacgat caacgtcgtt
    26581 gtgtcattcg gagcggagaa ataggagagg agtttttccc cttctcgagc cgggagtttt
    26641 tccaccgact tatagaccac cacgcggcgt tcggcgaaca ccggaatttc ggccgcgcag
    26701 gccaacacat cctcaaccga gcagtcgtcg ccgtgaaaaa tatcgtaatt gaatccggta
    26761 tccgccgccg ggcccaaggc tgcggcacgc aacgcggcca cggactggtc ccggagatag
    26821 tcttcttcgc cgaccacggc gtacagcggc atcaccccgc tgcgcgcgag ggattggggc
    26881 aattccagac tcatcactga agcgctcatc cgcagaaact ctccatcaat tatcgggccc
    26941 cataaggcgg tgtcccgccg gccggagcga cctcagcttt caccggacgc ttcagtgcct
    27001 tgtccaactc gcccgtatcc agaaagagca ggaatcgtga ggccaggtct tccgcgacga
    27061 accggcctgc ttgctccaac gcccgatttt gcagcacccg attgaattgc agatcctggg
    27121 tcagaaaaaa ctcagaggtt cccttcgcga cctggtacca gacgaccttt ttcgttttgg
    27181 cctcttcgat cctcacgctc acgagcattt ccacccggct ctcaaatgtc gtggtctggt
    27241 cgaaactcaa ggtcggcagc gtgatctgca tgatctgtcc ggacagatag agatcggccc
    27301 cggccgaagg gcccacgatt tccgccccgg ccccagccga gaactcccga cgcaggtaat
    27361 tggcgagctt gacctcgaag ttcgcctcgt aggtactgtt ggcaatagga aggatggcca
    27421 ggcgtggcgc gtgcggccgc gtcgtcgccg cctccggcgc accgccgacc gtgggaccgg
    27481 cgccctgcac acgaaactga tagccacagc cggggagcga gacactcaac gcaaagaaaa
    27541 taatcagcca caccacgcga ccaggaacct ccgggccggc acgtgctgcg ttgttcctgt
    27601 aacgttcagg ctggcgcacg gcatcacacc acgaagttga tcaatttctt ctcgacgtaa
    27661 atgaccttct tcggctcttt cccctgcagc cattcagcca cttctttctt agccagaggc
    27721 tccaactgct cgcgtgaagc cccggcatcg acctcgagtt ttccccggag ctttccgttc
    27781 acctgaatcg ggatcgtcag ccggtcgctg accaccaact cgggatcgaa ctgcggccat
    27841 ggctgctggc cggcgctcgg ttgttgaccg agacgttccc acaactcctc actcagatgc
    27901 ggcgcaaacg gcaagagcaa caacacaaac ggttccagca ggcggcgagg ccgctgctcc
    27961 agtttcgtca tctcgttcgt gaacaccatc atctgcgaga tcgccgtatt gaagcgtaag
    28021 gcgtcgatat cgtcggtgac tttcttaatg gtgtaatgga gcagccgcag ctgttcaggc
    28081 gtcggctcgg tatccgtaac cgccggactc aatgcgccct cgtcgccgat catcagccgc
    28141 cagacccgat cgagaaagcg cgtgatcccc tccacgccgc gggtactcca aggcttcatc
    28201 gattcgagcg gacccatgaa catttcatag agccgcaccg cgtcggcccc gaactggtcg
    28261 accatctcgt ccgggttcac cacattgccg cgcgacttgg acatcttctg attgtcttcc
    28321 cccaacacaa tgccctggtg caccagtttc ttgaacggtt ccggcgtact gaccaccccg
    28381 gcgtcgaaca agaccttgtg ccagaaccgg ctatacaaca ggtgcagcac cgcatgttca
    28441 ctgccgccga tgtagaggtc caccggcatc caatagcgct ccttcgccgg atcgaccacc
    28501 tgcacgggat tcttcggatc gatgaaccgc aggtaatacc agcaggaccc ggcccactgc
    28561 ggcatagtat tcgtctcccg tcgcgccggt ttaccggtga caggatccgt cgtctccagc
    28621 cagtcgtgga gattcgccaa ggggctttcg ccgctgccgg agggtttgaa attcttggtc
    28681 tcaggcaatg ggagcggcaa ttgctcctcg ggcaagggac ggggctcgcc gtcgacccat
    28741 acaatcggaa agggctcgcc ccaataacgc tgccgggcaa agagccagtc acggagtttg
    28801 taattgatcg tcttcttacc cctgccctga gtttcgagcc aggcggtcat ttttggaatc
    28861 gcttcgctgg gcaatagtcc atcgagagag caggatccat cgggcgtggt cgagttgatg
    28921 acacgtcccc gatccgtctc gacgaacgcc gccttatcga cctgcccacc ctcaatcacc
    28981 tctcggatcg gcaggtggta ttgcgtcgcg aaggcccagt cacgctcgtc atgtgccggc
    29041 accgccataa tcgcgccggt gccgtaactc atgaggacat agtcagcgat ccagatcggc
    29101 aggcgcttct ggttcaccgg attgacggca tacccgccgg caaagacacc ggtcttgacc
    29161 ttgtccagtt cctgacgctg tagatcgctc ttgcgggacg ccgcatcgcg gtaggccgtc
    29221 acctgctcac gctgctccgg cgtcgtcacc acgtcgacga gcggatgttc cggcgccaat
    29281 accatatagg ttgcaccgaa gagcgtgtcg gggcgggtcg taaacacgcg caaattgccc
    29341 gtcttgtcgg ccaagggaaa atcgacttcc gcgccgatcg accggccgat ccaatttttc
    29401 tgcatctcga gcgtgctggt cggccattcg accagggtca ggtcctccag caatcgctcc
    29461 gcataggcgg tgatcttcaa gacccactgc cgcatcggct tgcgaatgac gtcgaacccg
    29521 ccgacctcgc ttttgccgtc cacgatttct tcgttggcca gcacggtccc caaggcagga
    29581 caccagttca ccggcacttc cgccacgtag gcaaggcccc gttcgaacag cttcagaaag
    29641 atccactggg tccaacgata gtagtcctga tcggtcgtgc tgagctcgcg ttcccaatcg
    29701 taggagagcc cgactcgctt catttgccgt ttaaaggtcg caatgttctg cgcagtggtg
    29761 atggcaggat gcacgccggt tttcaccgca tactgttccg cgggcaaccc gaacgcatcc
    29821 caccccatgg ggtgcaacac gttgaagccg cgcatccgtt tatagcggga tacgatatcc
    29881 gtagcggtat acccttccaa gtgcccgacg tgcagacccg acccggacgg gtatgggaac
    29941 atatcgaggc aatagaactt gggcttggaa tgatcgtcca ccgcccgaaa cgggcgatgc
    30001 gtctcccaat aggtctgcca cttcgattcg agggcccgat gatcgtacgt cttgctcatt
    30061 gtggtatcgt catacgcagg gcaaccagtc ctgtagaatg aaagaacgga cgactctagc
    30121 acacgccgca cggagcgaca aggacgaggc ggcacaaaca gggaccgagt cgaccgcgaa
    30181 ggatcccccc tcccttgatc ccggaatcaa gtcctgttca aatgccggga caaccaatcg
    30241 ccgaagatcg accgcgcacg acctcacaga cttttgccaa cgccgatgta cgctacctac
    30301 atctttccgc gactcatgga ctgggtgctc cggggcgaac ggtttcagac cgaacgccgg
    30361 ctcctgctga cgccggtaca tggagtcgtc ctggaaatcg ggttcggaac cggactgaac
    30421 ctgccgcact accccacgac cgtgactgct ctgcacaccg tcgatcccgc cccgctcttg
    30481 cccgatcgag tcgccgtacg ggtggcacag gcttcgttcc ccgttcacat tcagcatgtc
    30541 agtgccgaac gattgcccta tgacgatgcc agcttcgact acgcggtcag caccttcacc
    30601 ctctgcacga ttccggatcc ggcgagggcg ctacgcgaca ttcgccgggt gctgaagccg
    30661 gatggccact tcctgttcct ggagcatggc cgcagcgacg atcccctcat cgcccggtgg
    30721 caggatcgac tcaacccgct gcaacacgtc ctggcctgcg gctgcaatct caatcgccgc
    30781 attgatcgac tggtactcga cgcaggtctc cggctggaac aacttgatcg ttattgtctc
    30841 cccggcgttc cgagaatcgg cggggaaatg tatcgcggca ccgcccgcct caacggacaa
    30901 tcgtccctgc attccagcct cgacacctaa cacactcgtc acgaccttgg cctcgcaccg
    30961 cgttcggaac cagccaaagc agtgacgaca tgaaacagga aaattgtatc cctatcggca
    31021 attttgtaga ctgcaccgta tagtgcacga tcctcgcatt cgtccatgca acagacggga
    31081 gtgacccgga tgtcctcctc acagtcgttc cccgacagag agcctccgtc gacctctcca
    31141 ttattcaaac ccatagattc agcccgggac agcagcatcg ccttgcaacg ggtgacgctc
    31201 ctttatgagg cgatgccggc cgcgctggtg gccggactcg gctgcgcact cgccctgatc
    31261 gcggtcaatt ggcatgtcgt cccgcacgac cggctgctcg tctggctggt gtaccatctg
    31321 atcctcgcgg caggccgata cgggctcgcc cgatcgttca cggccaacaa gccgggcccc
    31381 gccaatatcg aacgctggga gcgggcattt ctggtcggca cgacactcgc gggtctcggc
    31441 tggggcgcct cagctctgtt gctgtttccg gaatcctcac cggcccacca ggtttttctg
    31501 gccttcgtgc tcgcaggcat tacagccggc gccgcttcca ccctagccgc tcgcttcgac
    31561 gcctttctgt cgttctctct tccgatgttg acgccactca tcctgcgact cttcgccctc
    31621 gaccatgagc aagccctgcc gatggcgatc tgcaccatgc tcttctcgct gctcatggtc
    31681 tacacggcgt accggacgtc caacaccgtc ctggaaaccc tccggctgaa atatcacaac
    31741 gcccaactgg tggatcaact caccgggcaa ctgcaggaag gggagcagat ggaactgctc
    31801 ctcaccgtca agaccgaaca ccatcgcttc atcatggagt atgcccagga catcatctat
    31861 cggacagacc ggagcggccg tttcacgttc atcaatcccg cggtcatccg gctcctgggg
    31921 tatcacgaga cggagatgct cggccatcgt gcactggatt ttgttcatcc cgattaccgc
    31981 cgcccgacgg aacgcttcta cctccggcaa tttctccgga agacggccag cacctattgc
    32041 gaatttccgc tcgtcacccg gaaccaacag acgctctggg tcggacaaaa cgtgcagctg
    32101 ctgcaacgcg accaggaggt catcggattt caagccgtgg tacgcaacat ctccgccagg
    32161 aaacagaccg aggaggcgtt gcgcgtcagc caggaacgat accgtgcgct gttcgagagc
    32221 agccctgaaa tgctgctgac cgtcgacgcc cggggcacct tgctgacggt caataccacc
    32281 accgccgctg aattgggcta cgaggccgac gagctgatcg gtcaaccggt agcgcgaatc
    32341 gttcacccgg atgaccacgg cagcatggac aagcactttg ccgactctct ccgccggccg
    32401 ggagaagtcc tccgctggaa tttccggaag atgcgcaagg acgggagcct gctctgggtg
    32461 cgagaagccg cccgggccgt gcgcaactcc gacggacagt tcgatatcat catcgtgtgc
    32521 gagaacgtga ccgaacagaa gggcattgaa gatgccctga cccagacgcg ccaactcctg
    32581 gagtccatcg tcgaacacat tccccacatg gtgtttttaa aagacgctca agaattgcgc
    32641 ttcgtccagt tcaataaagc gggcgaagaa ctgatcggcc tgccgcgaga ggcgctgctg
    32701 gggaagaacg atttcgattt tttccctctt gagcaggcgg aattctttac cgccagagac
    32761 cgcgaggtcc tgaccggtgg cacgatgatg gacattccgg ccgaaccgat ccagaccaag
    32821 gatcatggcc tccggtggct ccacaccaag aagcttcccc tctacgacca ggccggcatg
    32881 ccgcgctatc tattggggat atcggaagac atcacggatc ggaaacaacg cgaagagacc
    32941 gaacggctgc gcttggggaa attagaaaca caacaagccg tgctgcatga attggcggaa
    33001 gatccggcca tccatagcgg acacccgcag caagccttcc cggccattac cgaacatgcg
    33061 gtgaacacgt ttgccgtcga acgagccggc atctggctct ttggcgagaa tcagtccgca
    33121 ttgatcctgc aggatctgtt cgaagccact cccaaacacc atctacaggg aaccacgctc
    33181 tcgaccggtg aatacccggc ctacctgcat gcgctcgaaa ccgaacccta ctcgctcgcg
    33241 gcccatgacg cgcagaccga tccccgcacg agtgaattga ctccgttgta cctcaccccg
    33301 cttggtatcg tggccatgtt ggacgccccg atccgcaggc atggtcgcgt agtcggcgtg
    33361 ttatgcctcg aacacatcgg tccggccaga gtctggacca cagaggaaca gacgttcgcg
    33421 gcctccctgg ccgccatggc gaccctgacc ctggaggcgt cggaccgtcg tcaagcccaa
    33481 gaggcgctgg agcaacatgt ccgtgaacgc accggagagt tgcgccggac gaccgcccat
    33541 cttcagacca tcatcgaaga gtcccccctg gcgatgatcg aattggacca agcgggcctc
    33601 gtcaccacgt ggaatgccgc cgccaccgcc ctcttcggct ggaccaaaga cgaggtgctg
    33661 ggcaaggagc tcccttatgt gcctcccggc caggaggagg tgtcggaaga cttgtggacg
    33721 gcagtgatga gcggcgccgc accacgcaat ctcgaactgc gccgccagaa aaaggacgga
    33781 actctggtgg atgtgaacat gtgggggagc ctgctccagg gtccggcagg ccaggcggtc
    33841 ggctccatcg gattcttcgt cgatatcacg aagcacaaac aactcgaaga ccagctccgg
    33901 caggcccaca agatggaagg gatcggccgc ttagccggcg gcgtggccca cgatttcaac
    33961 aacctgctca ccgtcatcaa cgggtgcgcc acgctcgtgt tggaccaggt gcgggccgag
    34021 gatccgcttc gtcgctcgtt gaccgagatc ctgactgccg gccagcgtgc ggcggccttg
    34081 accaagcagc tcctggcctt cagccggcgc caagtgttga cgttccaggt ctttgacctc
    34141 aacgaggcgc tctcgtcgat cagctccatg atcgaacggt tgatcggcga agacatcaac
    34201 ctgatcagaa atcttgactc gcggccctgt accattcgcg ccgacagagg acagatcgat
    34261 caagtggtac tgaatctggc cgtgaacgcc aaagatgcca tgcctacagg cggaacactc
    34321 acgatggcga cgcaggccct gctgattaca ccggacagcc ccgtgcccca tcccgcatta
    34381 ctcccgggag cctatgcgca cctgtcgatc cgcgactccg ggcaaggcat ggaccgcacg
    34441 accctgtctc atattttcga acccttcttc accaccaaag aagaaggcaa aggaaccgga
    34501 ctcggattgg ccacagtcta cggcatcgtc aaacaaagcc aggggttcat ctttgccgac
    34561 agcacacccg gcgaggggtc aaccttcgac ctctactttc cactggtgga ggcgccgccg
    34621 ctcgctgccg cgccgacact cccatcccgt ccacactgcg ggcgggagac catcctgctg
    34681 gtcgaggatc agcacgcggt gcggttgctg ctcacccagg ccctgtccga ttacggctac
    34741 accctgctgg aagcctccag cggacaggaa gcgctccgcc tggtcgccgc cgcccgcacc
    34801 cccattcata ttctactcac ggacgtcgta atgccgcaga tgacaggacc ggccttggct
    34861 gagcatctgc gtcggcaatg gcccgagcta cgggtgctct ttatgtcggg atatgccgag
    34921 ggaagcgtgt taccgacgtt tctcgcggaa ccgggcaccg gcttcattca gaagcccttt
    34981 ctccccactg agttggcgca gaagctccgg gaattgctcg atcccgcaaa ataggacacg
    35041 tgatggattg tgcagaaaaa ccggatgcgc gacaaacggc ttagccgggc aggatcgcgt
    35101 ggaaatgttc gatggaggta aaccgcgcgg agggaacggc tgaagcctga gagctcgacc
    35161 tggagcggtg aaaaatccga ccgatcccga aatcttccgc cgcagccaga cattgctcat
    35221 cgtcatcgat gtagagcgcc cgcgcaggat cgaatccgac caattgccgg caggtcggcc
    35281 aatactccga tcgcatcttg agccacccca cctcgaaggc atccacgata cgatcgacat
    35341 gccgatccag ccccgtcttg gcggttttca catccactcc tgcccgatgc gcgttcgtca
    35401 ggatcgtcac gcgcttcccc aatcgacgca aggatgagag aaactcctcg gcatcgggaa
    35461 gaaacccgat catgtggtcg agttcgcgat gcatcgccac cacatcgatg tctacccgct
    35521 ccgaccaata atgtaaatcc gtccaggcca attccccttc gacggaacga tacatggcca
    35581 tcaagcgatc ccgggcggcc tcgaaggtca gcgcatgctt cgccgcatac cgcctcggca
    35641 gttcttcctc gaaaaaaaag ttgtcgaaat gacggtcgag taacgtgccg tccatgtcga
    35701 gcaacacatc gtcgatccga ctccaatcga cggactgatg gggggcctct gagcgatttg
    35761 acagcatcct ggcagtatac cggagaccga cgcggcaaga cgagtcattg tgttcggtga
    35821 aatctctatg ctaccgtcgc gcaccatgtc acgcaccaaa caaacgcgcg gtcctgtggc
    35881 cccgctcttc acccttgagg gcggccggtt gcccattatc gccctcatcg ggc
//
LOCUS       DAORRQ010000044        34050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_77_length_34050_cov_276.620554, whole genome shotgun sequence.
ACCESSION   DAORRQ010000044 DAORRQ010000000
VERSION     DAORRQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77_length_34050_cov_276.620554"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..595
                     /locus_tag="PK224_13915"
     CDS             467..595
                     /locus_tag="PK224_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16887.1"
                     /translation="MKLPVCTPEQGMLESELRRAVKHQTLGLLKVMETAEGFYEMV"
     gene            complement(791..1234)
                     /locus_tag="PK224_13920"
     CDS             complement(791..1234)
                     /locus_tag="PK224_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRB16888.1"
                     /translation="MSLFLLDTNMASFAMKRQYPQVRAHLERAAKKHAIGISAVTQGE
                     LLYGLQRKGAPKELGELIRGFLVRVEVLPWDADVADTYGLVRAWCAQKGIVLSALDLM
                     IAAHAIHLNATLITNDQSFQRLAVPMVPQAPFQLRLLDWSEPDTR"
     gene            complement(1231..1500)
                     /locus_tag="PK224_13925"
     CDS             complement(1231..1500)
                     /locus_tag="PK224_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16889.1"
                     /translation="MAAAHRPKEHDTAKLFFSGRSQAVRLPLPYRIEGDEVYIRRDAA
                     TGDIILSRVPKDSWKEFCALVDRVDLPDDFLLDRDTRPAEERDLV"
     gene            2014..2220
                     /locus_tag="PK224_13930"
     CDS             2014..2220
                     /locus_tag="PK224_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16890.1"
                     /translation="MATEKRTRVQTFVSGAVKADIWVQSKKSSITYAVTVGVVDKKNR
                     QSGALKDIATVTAQAESWIKAHTG"
     gene            complement(2235..3215)
                     /locus_tag="PK224_13935"
     CDS             complement(2235..3215)
                     /locus_tag="PK224_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16891.1"
                     /translation="MTPQFAGSTDASLDEWGSRLFNVSAPTAPRKKSGGARLGSNAHA
                     GTGGRLRTTEARAQYVRQKLRDMVRRAPQVMVKLAKATKGMRGISNNLSYISRDGKLD
                     IEDQDGHIIQGKAAVADLKTEWQHGGFPIPEHSTMRDAFHLILSMPKLTDPISVQRAA
                     RDFAAREFSHYQYAMVLHTFETDPDPNPSPHPHVHLTVKTAGLDGTRLNPRKPDLQRW
                     REGFAEALREHGIDATTSSRIHRTTQERWTVRHLVDDATKTKRLDRSKRHTSTPQVQR
                     DVMQNYEQVMKALARSDRGDDRQLAADLVSHLSGKHRTIEKEKGRVKERE"
     gene            complement(3212..3754)
                     /gene="mobC"
                     /locus_tag="PK224_13940"
     CDS             complement(3212..3754)
                     /gene="mobC"
                     /locus_tag="PK224_13940"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HRB16892.1"
                     /translation="MSTRLNTICADLGELKQPWLTWCAKQKAKPSDALRQVLAKVIHG
                     GLPASVSRGTVLKDRPEKPTVRVEIHLTESEHRHVKPLAQAEGFPVTKWIIALIRARL
                     IGQPQLGQSELEVLARSNQQLLAIGRNLNQIAKALNTSPHDRSAFRLDLITELSSLIQ
                     THTNTVSSVMRGNLERWHIR"
     gene            complement(3917..4633)
                     /locus_tag="PK224_13945"
     CDS             complement(3917..4633)
                     /locus_tag="PK224_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16893.1"
                     /translation="MPHFRGSFLNPIEWNPLLWACLVAVPFYLFTVLWAGSPAAGIAV
                     GVGVGLFVALRRNSLIMSLRRRAEVTPSLIDWNVEIDHVHVGSLSDAELAAMRLAVLS
                     DLSTYAKQFLNMGRIALQACWSSFCVLCLMVFLLSMSILAVAPRELTHLLQTALVTHP
                     QHLTYLALLFVTSVCSITSLSLWVGWFGGQCYFGLTNYFSQSLTNLIRSRYHSSSSGS
                     LVLVRTSGSTLQFNTELSRL"
     gene            complement(4606..5628)
                     /locus_tag="PK224_13950"
     CDS             complement(4606..5628)
                     /locus_tag="PK224_13950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRB16894.1"
                     /translation="MIDLSGLEIKTAMAGVATETLGKPLELLLIDIEEDPNQPRRTFD
                     KESMGTMIESIRLNGVRTPVSVRTHPEKPGKWMLNYGARRYRASMAAGKTTIPAFVDQ
                     QHTDYHQVIENLQRDDLKPMELALFIQKKLSEGTSKAEIARTLSIPAATVTEHLSLID
                     PPECIETIYSKGKCTSPKTLYELRGLHKTFPKEVTRWCEKTEDITRGAVADLAVKLKG
                     TTTSDKEKSKQKHTAKTERPIVSESIHDRGEILEIQAGPQEVGVGQGSTARITVDAGA
                     TPIVYLEVKDRGKAVLILDRQSQNPWAVMVQFEKQEPEEVLGRDCTIDHVRFEIEVCR
                     TSEVAS"
     gene            complement(5625..6407)
                     /locus_tag="PK224_13955"
     CDS             complement(5625..6407)
                     /locus_tag="PK224_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRB16895.1"
                     /translation="MKTLVLANQKGGVGKSAIACQLGYFLSDMLKKRVLIIDLDHQGN
                     STKSIRASGLATVSAISSSCLLTDHVTTLEDAAFVLVPSDPELLRMERQADKHNTFAT
                     NLDGFLTAMEDRFDFAIIDTNPNPDIRVVASLVVSDFVLSPIQLNQEAVDGIAALRNQ
                     ILKIQSTLNPALKLIGILPNLVDPTPFQRANMEQLCTHFSSLMIRLANGKMAAISTRT
                     AVAEAQAAGIPIWRIGKTSARDAWGQIRPIFDSVVTSMEVSS"
     gene            complement(6939..7805)
                     /locus_tag="PK224_13960"
     CDS             complement(6939..7805)
                     /locus_tag="PK224_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRB16896.1"
                     /translation="MAIRISIANQKGGCGKTSTAINLADALRRMNYKVLFLDCDAQAN
                     ATKLLQMQPEANPGVTELILNPDIKLDDCLTATKLPNVSLIRGNIRLASVENRLREPS
                     LYPIPIGVLKGKVRKETDFDIVLFDTPPSLSLITMNALAASDFVIVPMESGSGFSFDG
                     LDDLLNIIATVRNSVQPDLAILGVLLTKHDSRRNVCQAVFQMVQDRFKGDCLSTTISM
                     STAAQKAELQRDTVLQYDRKCSASRDYIALANEVVTRLHNPTPTARVKSEVTKVIAET
                     MSPDATIEAVSA"
     gene            complement(7864..8358)
                     /locus_tag="PK224_13965"
     CDS             complement(7864..8358)
                     /locus_tag="PK224_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16897.1"
                     /translation="MDKLILSAPFEELDRRSLLSFVQQMPSLGYLQDFIDKVIAVIEQ
                     KKPSPKPIADPVAFLFGCLKKMEINPPPGWKSRAVRMLEEEERRLKTEAEELRAAGNR
                     VERQRCELYFLRLSPEKQSEITREIEAVGERDIPMIQQAKREQRYLELIRERMRQEAT
                     ASSV"
     gene            complement(10260..10913)
                     /locus_tag="PK224_13970"
     CDS             complement(10260..10913)
                     /locus_tag="PK224_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16898.1"
                     /translation="MTEPNSWDEDKELAAFLPPEAEAEASHLRFLTKLLQTLDHPTYR
                     AMSPGKQQLYLQLLRWSHGQQQEAIEASRVQMSAWTGLAWDTIKKYIPSLIEDKLVQI
                     VRPATSINPAMYEVYWLPPPPRPTLDQTAPLETIRCYVDQFDAEDLAEYQRLTPLLTG
                     AERKQLHSEVSWSLRDLGIPWNYELMKKLVMWQFLIHSPYRRQLEAKHPEWFSTPAV"
     gene            11315..12484
                     /locus_tag="PK224_13975"
     CDS             11315..12484
                     /locus_tag="PK224_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB16899.1"
                     /translation="MPILPAKQAAKPWALHRSPRRIKKDDVPLLRWPDQAPLGSPAAD
                     AHIHTIVESFLACFRTPTTIVSYRKALRGFFDWWGRDDQADPLTVPLLRAYQTYLIHT
                     VEPRTVNFYLATLRSFCGWMVDQGEFPWNPMDEIRSLRIPKGFVRDSLSEDEIRQLLQ
                     LMPRGGATKPAQELALRNYAMAVLWCAVGLRSIELARAQRRHLDFDQGHHLLKIHGKG
                     DDMAGKPVVLEPWVMVPLTAYLEFHDQWHQADAAGHARPAHDHHPLFCWTRGPGAYRS
                     RKHNPALTRSCRPIGVRVIQDMMRDAMGQAGLLNHTVGDTQNVRRITPHSLRHSAATI
                     ALERGAPIHQVQDMLRHADIQTTMLYTHNKERITHAAEHRIPDFTTPPDQAINTD"
     gene            12622..13254
                     /locus_tag="PK224_13980"
     CDS             12622..13254
                     /locus_tag="PK224_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16900.1"
                     /translation="MKKPGWKKWKKAAVVLLFAGAGLFTYSHYLPDPQQPPDPLCEVT
                     ASQIGPECPQATSIEDRIHNIENALIEIVADLQQLSDHLCPPDEHTEEDPIKKQLEAL
                     KHNLITSLYPSDTTHSDEVIIRVIQSRLERLHSYNDKVTHQILESGTIRPASKAEVES
                     LVERLDTRLKQLVDEFTHPPDFSHHHATLETFHETMEGLIEVVELLKNLC"
     gene            complement(13267..14535)
                     /locus_tag="PK224_13985"
     CDS             complement(13267..14535)
                     /locus_tag="PK224_13985"
                     /inference="COORDINATES: protein motif:HMM:NF002955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HRB16901.1"
                     /translation="MPSVFALVDCNNFYASCERLFNPTLAGQPIVVLSNNDGCVVARS
                     NEAKALGITMGVPEFQIRPLLQAHHVQVFSSNYRLYGDLSQRVMETLEQFSPDLEIYS
                     IDEAFLSLAGFERRNLTDYGRTIRKTVKQWTGLPVSVGIAETKTLAKLANRIAKRTAD
                     TDGVFDLLACPDREALLNGVAVGDVWGIGPNSARLLMQRGITTALQLREADEPWIRKH
                     MGVVGMRVVAELRGQSCLALEDCPPPKQGITCSRAFGRSVNTLAEMEEAVSSYVSRAA
                     EKLRGEGLAATVLTVFIMTNAFTEEPQYRNSVTCELPVGTDSTSELIRAALKGLRRIY
                     RDGYRYKKAGVMCTALVPAGQVQPDLFDQQDRPKSKRLMAALDAINDRWGAGTLDYAS
                     NGLTQAWQTQCHHRSPAYTTNWAELPMAKA"
     gene            complement(14543..14974)
                     /gene="umuD"
                     /locus_tag="PK224_13990"
     CDS             complement(14543..14974)
                     /gene="umuD"
                     /locus_tag="PK224_13990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HRB16902.1"
                     /translation="MPMNVDAVYAPDLSTRYALPVFLGRLPAGFPSPADDYIEGKLDL
                     NRHLIKHPAATFFVRVTGDSMIGAGIHSGDLLVVDRSLDAVDGNVIVAALDGELTVKR
                     LFKRGEILRLLPANTDYQPIEILTQQTFEIFGVVTNVIHAL"
     gene            complement(15010..15684)
                     /locus_tag="PK224_13995"
     CDS             complement(15010..15684)
                     /locus_tag="PK224_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRB16903.1"
                     /translation="MCGRFTQTASPAVIAEEFGVAVPPLFTPRYNIAPSQPVAAIRIE
                     PGTTARQLVELRWGLIPSWAKDPKIGHQCINAKAETVAEKPSFRAAFKARRCLVIATG
                     FYEWQVRGKAKQPMWIGLKSSHPFAFAGLWEQWQPPEGAAIESCTILTTEPNELLRPI
                     HNRMPVILSPTSYDQWLDPSFQQAEPLRALLRPYPSEDLQAYAVSTLVNNPRHEAPDC
                     LEPLPA"
     gene            15873..18722
                     /locus_tag="PK224_14000"
     CDS             15873..18722
                     /locus_tag="PK224_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRB16904.1"
                     /translation="MLTAYHAKYFAHELTKRCPSDSIEKLASALVDAQVDLNPHQIEA
                     ALFAFRSPLSKGALLADEVGLGKTIEAGLVLSQKWAERKRRVLVITPSNLRKQWHQEL
                     QEKFFLPCCILETKSYTATVRQGHVRPFECDDIIICSYQFARSKAAEISLMRWDLVVI
                     DEAHRLRNVYKPTNVIANTIKQALKDAPKLLLTATPLQNSLLELYGLVSVVDDRIFGD
                     LKSFREQFANLHNKPTFDILKARLQPICKRTLRRQVLPYVRYTKRLPMMQPFTPAENE
                     DRLYHLVSDYLRRDNLQALPSSQRSLMTLVLRKLLASSSFAIAGALHSMSQRLRERLR
                     LAEGPNPLEEALEEDFEALDEMAEEWEEEDAVPEALTAADRKAIQQEIDDLEGFQKLA
                     VSITHNAKGQALLTALRTAFGKAEELGAAQKAIIFTESRRTQEYLLRVLADSPWKDHV
                     VLFNGSNNDLRSKEIYQEWLQNHQGTDRITGSRSADLRSALVDYFKDQGKIMIATEAG
                     AEGINLQFCSLVVNYDLPWNPQRIEQRIGRCHRYGQKHDVVVVNFLNQNNEADQHVFR
                     LLSEKFKLFEGVFGASDEVLGAIESGVDFEKRIVDIYQRCRTPQEIKTSFEQLQSELG
                     TQIDEAMTRARQLLFEHFDDEVREKLRVSDESSRLYLNRYERILMQLTRFELRDHAEF
                     LSDTSFRLNSCPFRGEIPLGLYELPRRSGEAHLYRLAHPLAEHVLEQGKNRQLDPAEL
                     TFEYGKHDGKVSVVEPLVGKSGVLTLSLFTVEALDQAEDYLISTGVTDNGEVLDEEAV
                     RRLFSLPATVTQPVKSSIEHPALSTRTEERQDAIRRHISRRNAEFFEIEADKLDAWAD
                     DLKVGLEREIKEFDRQIKEARRAAVAALTLEEKLAGQKQIKAIEAERSKRRRALFDAQ
                     DEIDRRREQLISEIEGKLQQKVNFQQLFAVRWQVR"
     gene            18924..19670
                     /locus_tag="PK224_14005"
     CDS             18924..19670
                     /locus_tag="PK224_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HRB16905.1"
                     /translation="MAQTSRIEWTEATWNPTVGCTKISPGCKHCYAEAMAKRLHAMGT
                     PGYENEFRLTVLPDRLNEPLRRKKPTIYFVNSMSDLFHKNVSDGYIEQVFDVIARCTH
                     HTFQVLTKRAERLASYFLRRTVPKNAWIGVSVEDQICGLPRIDALRHVNAHIRFLSVE
                     PLLEDLGEIDLTDMHWVIVGGESGPNARPMKREWVENIKRQCQEQGVQFFFKQWGGWG
                     ADGKRRSKKKNGRLLDGRTWDEMPPLSTRI"
     gene            19680..20948
                     /locus_tag="PK224_14010"
     CDS             19680..20948
                     /locus_tag="PK224_14010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HRB16906.1"
                     /translation="MSRKHYDWKNGPDVIQQHSIAKHRILQAYLAAYFQTLVSSPSQD
                     VLRLTLVDGFAGGGLYVHNDTKELVKGSPFICLNATKEAGFLINRERRKPVNLDVSYF
                     FVEADRHAHLHLEKTLREEGYGPELNNSIYLRHAKFQDEADSIIQFIKQKSPRNGRSI
                     FVLDQYGYKDVPTELVQKIFRNLPSAEVILTFGVDSFLNFASDTGLTARLLDEIGVPD
                     LLQGRTIDEIKGSERNWRLFIQSGLYRRLVTRCGAKHYTPFFIRNKKGHGDYWLIHLS
                     QHHRARDVMTEVHWDNNNYFIHYGGPGLDMFHMVGYDPDHDSAQKGQFELGFEFDDVA
                     RNASIASLMEQIPRLIYANNEGASFGVLFATTCNESPASAKIYRSAIEQLIEHRVVEV
                     ISPDGVSRRSAQQIQSSDQIVAPGQRSLFH"
     gene            21019..21582
                     /locus_tag="PK224_14015"
     CDS             21019..21582
                     /locus_tag="PK224_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16907.1"
                     /translation="MKLRFLSSLLIFVSSYFPLALIFIIKDLDNETFLPMHALTAAII
                     TVLVLIACGIVLHAARKIESGVPVEVTKVSNKSGDMFTYTIPYMISFYNFNLGDWKTL
                     LSLFVFMTLMFALSYRTQNVFINPVLALAGYGLYDCQFRDGSREVQGLLISRYEFQLG
                     DTCIVEQLSKSQYFVSSVQAKEKKDGS"
     gene            21572..22510
                     /locus_tag="PK224_14020"
     CDS             21572..22510
                     /locus_tag="PK224_14020"
                     /inference="COORDINATES: protein motif:HMM:NF027488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HRB16908.1"
                     /translation="MAAKIAKKIADLRQTDFKPWTTTLWLVKRRLDVHKKAHYSVLRV
                     DVDKKLQDKLKKAVTERIHGVDLKLEAYEFLTDDQDGRLFTIESAETDFIKIQAEVDK
                     GLENKKVEKYEDLLDSWACVIKLEHQGSVLYGVRKINKFARATKVKAVSYFIFENKKL
                     VDLEGKQVFTLDTHIDFFAYDGTMFIANKKEFESVLNFRNGMEKNRDAILEEFVTLKV
                     VVDVEVIRRVVGGNLHLLRKMSSIQKSGYYKDKIFLVELTKKNVEKGWNLEVEHGVIV
                     VDESNVELVLKLLNNERVESQINQEVFDAVVKKKVG"
     gene            22524..24158
                     /locus_tag="PK224_14025"
     CDS             22524..24158
                     /locus_tag="PK224_14025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRB16909.1"
                     /translation="MNKKQKLELTWIGKENRPKLEPRILLEDREKSCHAPHRVTDRDL
                     FDNRLIFGDNLLALRALEQEFAGKIKCIFIDPPYNTGSAFSHYDDGIEHSIWLSLMRD
                     RLEIMRQLLTDDGSLWITIDDNEAHYLKVLCDEIFGRSNFVATCVWEKDKGGRGDADI
                     SLSHDNILVFSKTRTVWSNSRNLLSRTETQLGRFKNPDNDPRGEWRQGDDGTAKSGTE
                     KQRFPVTLPSGRVVTPPPGRYWAFSPHTLEQARAEGRAYFGVDGDRLPIIKRYLSEVR
                     EGVAPRTWWSADEVGTNQQAKRDHLNKLLPDIEPFATPKPEGLIQRILHIASNQGDWV
                     LDSFAGSGTTGAVAHKMGRRWIMVELGEHCHTHIIPRLKKVIGGEDKGGITESVGWKG
                     GGGFRYYHLAPSLLEKDKWGNWVINKAYNPAMLAQAACKLEGFVYEPSDAVYWQQGHS
                     TERDFIYVTTQNMSHDQLQALSDEVGEERSLLVMCSAFRGKPDKYPNLTIKKIPKTVL
                     TRCEWGHDDYSLKIENLPKAPIPPGQMELLGEEAKS"
     gene            24155..26833
                     /locus_tag="PK224_14030"
     CDS             24155..26833
                     /locus_tag="PK224_14030"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRB16910.1"
                     /translation="MNRHVNAIAGRLSLRPPQRESLAILDRVTEIVPPKKGADVAAAL
                     AAIKSEFPTVTDFEREFPSLCFALATGVGKTRLMGAFISFLHLAHGINHFFVLAPNLT
                     IYNKLIADFTPNTPKYVFTGIAEFATEPPTIITGDNYEAGVAARLTSLPGFGQEVHIN
                     IFNISKINSEVRGGKAPRIKRLSEYIGQSYFEYLAGLDDLVLLMDESHRYRASAGVRA
                     INELKPVLGLELTATPFVESTQGPVPFKNVIYDYSLAKAMADGFVKEPAVVTQKNFNP
                     SQFNAAQLEQIKLEDGVRLHENTKVELETYARQTGQRVVKPFMLIIARETTHAGQLMK
                     LIQSPEFFEGRYRDKVIQVDSSKTGAEEDEMVQRLLAVESPDEPTEIVIHVNMLKEGW
                     DVTNLYTIVPLRAANARTLIEQSIGRGLRLPYGKRTGVSTVDRLNIVAHDRFQEIIDE
                     ANKPDSMIRLTQVILDPATDLQKMRTVVAQSKIEEQLHGASLTGPADGPPQTTPIFAG
                     EAERGIAQATYKIIQKYENLPSSNDLLKPEIQAKIVEEVKQSITPSQQALPGMATVPS
                     IAAVVAKTAQLVVQQSIDIPRILVVPKGEVTTGFHSFTLDCANIHYQPVERDLLIQIL
                     RTHEQETLSCGARMQPEQRLEDYLVRGLVDLDDISYDEHSDLLYDLAGQLVRHLRSYL
                     SEEEARNVLIYHQKQLAAFVHAQMQAHQWEKATGYDVVVSKGFTDLKPSAYTARDGEP
                     IQDFRQTVEDRSRIGQMLFGGFQRGLYSVQKFDSDTERRLAIILDRNAQKWFRPSSGQ
                     FQISYKQGVSEHDYQPDFVAETADCIYMLEPKAKNEMTTPEVLAKKDAAIIWCTRATT
                     HALSNGGKPWKYVLIPHDMVDQNMTLAGLVKQFATE"
     gene            complement(27324..29228)
                     /locus_tag="PK224_14035"
     CDS             complement(27324..29228)
                     /locus_tag="PK224_14035"
                     /inference="COORDINATES: protein motif:HMM:NF023505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HRB16911.1"
                     /translation="MMTATYSPDDNKLRLYSVSRLDNETYARVKAAGFIWAPKQRLFV
                     APMWTPEREDLLIELAGEIDDEDASLVDRAEERAERFEGYQENRAKDAERARKAVAAI
                     ADNIPLGQPILIGHHSERHARRDATRIENGMRKAVDMWETSQYWKSRAAGAIHHAKYK
                     ELPAVRARRIKGIEADQRKTQRTKDEAEKFLKLWSPEGLTHEQALAVANDSSFHLPRK
                     DGDRPEWQYTPTAYDALTGSSPTLYAPRTLEDVLEAAKTLYPGIIARCDRWLTHYANR
                     LEYETAMLAEAGGLVSDRQKFEIGGQILRRGKWFIITKVNAGSVSVIGHFAGTIPFDE
                     IKEYKLPQAGDTEKVKAATKLPPLCNYPGEGFTHMTKEEMKADRWRMYSDSPKTGVIK
                     ATDTHGAHRVQQTRGKDAWSSVPVYVTDAPRKDPPAKATPPALPEREGTAPTIRQHQP
                     PAPTVYDAMKDSLKAGVQTVSAPQLFPTPPAIVAQMVEKADLQPGQRRLEPEAGTGHL
                     IEAMADVPGEIVAVEINAALVTQLRRRWDDGSVDIRLGDFLTCTGELGTFDRILMNPP
                     FGSADDVKHILHARHFLKPGGKLVALCANGPRQQEQLKPIAETWEILPEGSFQEAGTN
                     VRVALLTIQG"
     gene            complement(29307..29750)
                     /locus_tag="PK224_14040"
     CDS             complement(29307..29750)
                     /locus_tag="PK224_14040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRB16912.1"
                     /translation="MNYRIPVVKLSMIRDGSITTDRRTLSDSHSAAALCRSIIGDNDR
                     EEMLAVILDAKHKINAVHSISVGTLSLSLVHPREVFKAAVLCNAAAMIIAHNHPSGDP
                     TPSQEDRLLTKRLRECGELMGISILDHLVIGDADRYYSFADNGVL"
     gene            30750..31952
                     /locus_tag="PK224_14045"
     CDS             30750..31952
                     /locus_tag="PK224_14045"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRB16913.1"
                     /translation="MAFTLHVVPNTFSTYGIQIPDLLSYSGFSRQPCSFLTRECYVKE
                     VNADVNLNAFGKALGEAYQQLSQAQRHLERCGFILDQPEGWGYFFGQQSGGRSRSDYY
                     GDGHISAIPPQVMKTAEDDVFHFVFTWIKNSESEKGWTIHYRPKHSPLSVELQGVFHF
                     LGIDEFRECPQFEFESCSWRHIEFREGGDHIFENGNASVAHGWFDSHQLNFSPGIKAL
                     LTAQATMEEVGMGFLPSFAQPEMRRTQEITRHITSPSTMAPKPASSPMFDVAISFAGT
                     ERKLAQELATRVKTAGFQVFYDEFFPEELWGKNLGEFFHEIYSKRSRFCVIFVSAEYL
                     EREWTVHERRSAQERMLKDKGNEYILPIKVDDVELPGIPSTIGYLPISTGIDKIADAL
                     ISKLKNKR"
     gene            32022..32876
                     /locus_tag="PK224_14050"
     CDS             32022..32876
                     /locus_tag="PK224_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16914.1"
                     /translation="MSDLPQSPDDKAIIERLRSELAKSEPTRHKRILEKFVLAALGSI
                     PWIGGFLSAAATYKTEEGSLKQDSLQTQWLEEHQQKIGLLREALEEIRQRFEKLGASI
                     EDRIQSEAYLALVRKSFRAWDEADTKEKRRYIANLVTNSAGTRVCSDDVVRLFLDWLE
                     LYHEIHFAVIREIFENPGSTRFGIWSDLYGDTPREDSAEADLFKLLIRDLSTGGVIRQ
                     ERDVNQLGQFVRKTPQRARRGTAPTTIESAFEDSKPYVLTELGKQFVHYTMSEVVSRI
                     DGPNVRPS"
     gene            complement(33299..33877)
                     /locus_tag="PK224_14055"
     CDS             complement(33299..33877)
                     /locus_tag="PK224_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16915.1"
                     /translation="MDAASVALLFDRAAHFSKVVSAFGGSGCAWSYTFPDGETHRYII
                     NGIKSPDQLEHEVLTLAIWLWSLKDYLKGRAATLGRDPQDVEAYASGDPSLALSADIA
                     NAAKHGKLTNSRSGKWPKLGRLRYEIPQEAMRSVTFRAFEVETDVARPDLVTFELPIL
                     DSNGLEIGDALQLMTRALSAWERFNKKLERAD"
BASE COUNT         8078 a         9166 c         9142 g         7664 t
ORIGIN      
        1 gtcggaagca gccgttcaga acaatcgagt gccgatcctt atccgagtcg gggagtctgt
       61 caatattcaa caactcattt aggcttgggc agttcggtcg gctggtttgg agcatttcga
      121 gtggctggtt tccgtcataa tcggtggccg acttgcgtcg gaatcagtgg ccggcttggg
      181 tcggaatacg cactgtgtcc agtttccaac tttaagtggg tcaaaaatgg gtgacgaccc
      241 tcaattttcc agaaaattga ttccgcctag tacacaacat tcccaccact aacagtactc
      301 aagcgatact tcacactccg ggtagtcaag acgagaccag cccgctcgaa agggattgaa
      361 gcccgcaagg gccgaaatca cgtgaggggg ttcggtccac gaaagcccgg ccccaggtac
      421 tggggtgagc agagaacatc atcttttcat acaggaattg tgatcgatga agcttcccgt
      481 atgtacccct gagcaaggca tgttggaaag tgaactcagg cgggccgtca agcatcagac
      541 gcttggactc ttgaaagtga tggaaacagc agaagggttt tatgagatgg tttaactgaa
      601 ctgagcaaaa gaaacagatt ggtttctcac gactcgacga gagccacgtt cgccccgcgt
      661 gttcaaacgc ttgaatcggt tgaacgcgca tgtgcgaaat atttcaccca cggtcacggt
      721 tcatctgcgg cggatgtcac ggtgatggct atcctacgaa gcattcgtaa tcctggaagc
      781 ctccaacagc tcaccgtgta tcgggctcgg accaatcgag aaggcgtaac tggaagggcg
      841 cttgaggcac catgggcacg gccaatcgct gaaagctctg atcgttcgta atcaacgtcg
      901 cattcaaatg aatggcatgg gcggcaatca tcagatccaa cgccgacaac acaatcccct
      961 tctgggcaca ccaggcacgg acgagtccgt acgtgtcagc cacgtccgcg tcccacggta
     1021 acacctcaac acggacgaga aagcctcgta tcagctcccc tagctccttc ggcgcacctt
     1081 tccgttgcaa tccatagagc aattcgccct gtgtcacggc agaaatgccg atcgcgtgct
     1141 tcttcgccgc ccgttcgagg tgggctcgaa cctgcgggta ctgccgtttc atggcaaaac
     1201 tcgccatatt cgtatcgagg aggaacagac tcatacgaga tcccgttcct cggccggccg
     1261 cgtatcacga tcgagtaaaa aatcatcggg caagtccacc cgatcaacca aggcgcaaaa
     1321 ttctttccac gaatctttcg gcacccgtga gagaataata tcgcccgtcg cggcatctcg
     1381 tcggatatag acctcatccc cttcaatgcg atacggcaag ggcagtctca ccgcttggct
     1441 tcggcccgaa aaaaacagtt tcgccgtgtc gtgctccttg gggcgatgtg cagccgccat
     1501 agttgctccc tccctccgtg ctacatatca taagatatac cgccacgata tcaagagttc
     1561 ggcagaagat caagcccaaa accgtgagca tgactcaagc acccaatggc ctgactccct
     1621 catggctcat acgccaacat tccacggtga aaaacatctg ctatggtttt catagcatcg
     1681 atgcgaggta caccatggaa accgctgctc ccgcttccta tcgcctggtc cctctgcctg
     1741 ctctggcccg tcctgccaca atagcatcgc caacacccag ccgtgagagc ggtcgactct
     1801 ggttcattcc tccgtcgcct ggtccgcccg gtaggcctgc gctttcctgc cgctgttgca
     1861 cggaccccgc caactccggg ccgttgtcat ggcccgtgct gacacagcgt cagccgcgtt
     1921 caggcaaccg ggctcctagg acctcctccc gtgtgccagg actctcaagt tttgcgcgag
     1981 agtcccgatc acactaatca aggaggtcca gagatggcga cggagaaacg aaccagagtc
     2041 caaacattcg tatctggggc tgtgaaagcg gatatctggg tccaatcgaa gaaatcgtct
     2101 atcacgtatg cggtgacggt cggcgtcgta gacaaaaaga atcgtcagtc aggagcactc
     2161 aaagacatcg ccaccgtgac cgcgcaagca gaatcctgga tcaaagccca cacgggatga
     2221 tcccgaacgc gggattattc ccgctctttg acccgcccct tttccttctc gatcgtgcgg
     2281 tgctttcctg ataggtgact taccaaatct gcggccagtt gtcggtcatc gcctcgatcg
     2341 gaacgcgcta acgctttcat cacctgttca taattctgca tcacgtcgcg ttgaacctgc
     2401 ggcgtcgagg tgtgtcgctt actccgatcc aggcgcttgg ttttggttgc gtcatcgacg
     2461 agatgccgaa ccgtccaccg ttcttgggtc gtgcgatgaa tacggctgct ggtagtcgcg
     2521 tcaatcccat gctcccggag cgcttccgca aagccttctc gccagcgttg gagatcaggt
     2581 tttctggggt tcaaccgtgt cccgtcaagt cctgcggttt tgaccgtgag atggacatgc
     2641 ggatgaggcg acgggttggg gtccgggtct gtctcgaacg tatgtaagac catcgcgtac
     2701 tgataatggg aaaattcacg tgcggcaaag tcacgggccg cacgttgaac cgagatcgga
     2761 tcggtcagtt ttggcatgga caggatgaga tgaaaggcat cgcgcatggt ggaatgttca
     2821 gggatcggaa agcctccgtg ttgccattcg gtcttgaggt ctgcgacggc cgctttcccc
     2881 tgaatgatgt ggccgtcttg atcttcgata tccagcttcc catcccgtga aatgtagctg
     2941 agattattcg agatcccgcg catgcccttt gtcgctttgg cgagtttgac catcacttgc
     3001 ggggcacgtc gcaccatgtc acggagcttt tgccgcacgt actgcgcccg cgcttctgtg
     3061 gtccgtaatc gtccgcccgt acctgcgtga gcgttggacc ccagtcgtgc cccaccagat
     3121 tttttccggg gcgcggtggg tgcggagaca ttgaagaggc gactacccca ttcatctaag
     3181 ctggcatccg tgctcccggc aaattggggc gtcatcggat gtgccaccgc tcaaggttgc
     3241 ctctcatcac actcgacacc gtgttcgtat gcgtctgaat gaggctggaa agctccgtga
     3301 tcaggtctag tcgaaacgcg ctccgatcat gaggggaggt attcaaggct ttcgcgatct
     3361 gattaagatt ccgcccgatc gccaacagct gttgattgga acgggccagg acttccaatt
     3421 ctgattggcc caattgtggc tgtccaatga gtcgagcccg gatcaacgca ataatccatt
     3481 ttgtgacggg aaacccttcg gcctgtgcga gcggtttgac atggcgatgc tcagactcgg
     3541 tgagatgaat ctcgacccga accgtaggct tctcggggcg atctttcaac acggttccac
     3601 gagaaacaga ggcggggaga cccccgtgta tgaccttggc caagacttgc cgaagcgcat
     3661 cgctcggctt ggccttctgc ttggcgcacc aggtaagcca cggctgtttg agttcgccca
     3721 ggtcggcgca gatggtgttc agtcgtgtac tcatagccgt tccctacgag ggcgttcctt
     3781 tcgtgtgggg gactgatttc aagtgtggag gactataaca aaaccacaga tgtaatacaa
     3841 cacacggcga tagccatatc ctgcaaagta ttttcattgg ccaaactctt agtcatgact
     3901 aagaccatac ggccaactac agccgggata actctgtgtt gaattggagc gtagaaccag
     3961 aagtccgcac gagcaccaag gagccagaac tagaagaatg gtagcgactg cggatcaggt
     4021 tggtaagtga ctgggaaaag tagttggtga gcccaaaata acattgccca ccgaaccacc
     4081 ctacccacaa cgagagcgaa gtaatggaac agacggaggt gacaaacagc agagccaggt
     4141 aggtaaggtg ttgggggtga gtcactaagg cagtttgtaa gagatgcgtg agttcacgag
     4201 gggccacggc caggatgctc atggagagca aaaagaccat gagacagagc acacagaaag
     4261 atgaccagca agcttgcaac gcaatacgtc ccatattcaa aaactgcttg gcataggtgg
     4321 agagatcaga gagcaccgcc aaccgcatgg cagctaactc tgcatcactc agcgacccaa
     4381 catggacatg atcaatttct acattccagt cgatcaggga aggggttact tctgcgcgtc
     4441 ggcgtagtga catgatcagg gaattccggc gcagtgccac aaagagcccc acccctactc
     4501 caaccgcaat gccagcggcg ggactcccag cccataacac cgtgaagaga tagaacggta
     4561 ccgcaacaag gcaggcccac aacagggggt tccactcaat gggattcaag aagctacctc
     4621 tgaagtgcgg catacttcga tctcaaaccg tacatgatcg atcgtacagt ctcggcctaa
     4681 gacttcttcg ggttcctgct tctcaaactg taccatcacc gcccacgggt tctgactctg
     4741 acggtccagt atcaacacgg cttttccgcg gtctttgact tcgagataca cgatgggagt
     4801 tgcgccagca tccacagtga ttcgcgcggt actcccctgc cctactccaa cctcttgcgg
     4861 ccctgcctgg atctcaagga tctcccctct atcgtgtatg ctttcactga cgatcgggcg
     4921 ctctgtcttc gcggtgtgct tttgcttcga tttctctttg tcggaagtag tggtaccctt
     4981 gagttttacc gcaagatcgg ccacggctcc tcgtgtaata tcctctgttt tctcacacca
     5041 gcgggtgact tctttcggaa acgtcttgtg aaggccgcga agttcataca acgtcttcgg
     5101 cgaggtacat ttgcccttgg aataaatcgt ttcgatacac tccgggggat caatcagtga
     5161 cagatgttcc gtcacagtgg cagccggaat actcagcgtc cgtgcgatct cagcctttga
     5221 agtgccctcc gacaatttct tttggatgaa taaggccagt tccataggtt tcaggtcgtc
     5281 acgttgtagg ttctcgatga cttggtgata gtctgtgtgc tgttgatcga caaaggcggg
     5341 aatcgtggtt ttcccagcgg ccattgacgc ccgatagcgc cgggctccgt agttcaacat
     5401 ccatttgcct ggtttctcgg gatgcgtccg caccgaaaca ggggtacgga caccattgag
     5461 acgaatactc tcaatcatgg tgcccatgct ctctttatcg aatgtgcggc gcggctggtt
     5521 cggatcctct tcaatgtcga ttaacaggag ttcgagcggc ttaccaagag tctcagtcgc
     5581 aaccccagcc atagcggttt tgatttcaag tccagaaaga tcgatcatga tgagacctcc
     5641 atgctcgtga caaccgagtc aaaaatcggg cgaatttgtc cccaggcatc ccgcgcactg
     5701 gttttaccaa ttctccaaat agggatgcct gcggcctgtg cttccgccac ggctgttcgt
     5761 gtggaaatgg ccgccatttt cccattggct agtcgaatca tcagactcga aaaatgcgtg
     5821 caaagttgtt ccatattggc acgttgaaag ggcgtggggt cgactagatt agggagaatc
     5881 ccgatcaatt tcaaggccgg gttgagtgtg ctctgaatct tcagaatttg attccgcaag
     5941 gccgcgatgc catcaacagc ctcctggttc agctggatcg gcgatagcac aaaatctgaa
     6001 acgaccagcg aggcgacgac gcggatatct gggttcgggt tcgtgtcgat aatcgcaaaa
     6061 tcaaaccggt cttccatcgc ggtcaaaaac ccatcgaggt ttgtcgcaaa agtgttgtgt
     6121 ttatccgcct gccgctccat ccgtaatagt tccgggtcag agggaacaag tacaaaggcc
     6181 gcgtcttcaa gcgtcgtcac atgatcggtc aggagacaac tcgaagaaat cgcggagacc
     6241 gtcgccaggc cggacgcccg gatgcttttc gtagaattgc cttggtggtc gaggtcaata
     6301 atcaggacac gctttttgag catgtcggag aggaagtagc ccaattgaca ggctatcgcc
     6361 gatttaccaa ctcccccctt ttggttggcg agcaccagtg ttttcatagg cccccttcgt
     6421 gaatcttgag tcgttcaagt tctgtgaatg ccaagcaaca tgtacgaaat cggcgagcga
     6481 ttcggctccc ggcggtacca ggtatttttg gggacaccaa ctttttcgcg actgtggtct
     6541 gccataatcc taatttggac taaccctcca ggatttcctt gtctcaatcc tctcgcctct
     6601 ggataacaaa gagtgtccca aaccagacat gatagtgcct caccaccccc ggcagtggga
     6661 acacgagaag aatcgacgtg ttgattgggt aggccttgag gaggcacaag attaagaccg
     6721 tatctcagtc gagaccaaga aagaccgtca aagtggtacc ggttccaaac tgggaaggtt
     6781 gaagcagacc tatggccctt caatcctctc ttcttgaaga tgtctgatac cgatcgagcg
     6841 ccgtgacact agatggaatc ccctcttgaa cggctaggat ctcgttaacc tgattccgaa
     6901 agagtcggac agtcggttca acatcaatgg cgacggcatc aggcagatac cgcctcaatc
     6961 gtggcatcag gagacatcgt ttccgcaatc accttcgtta cctcagactt cacacgcgct
     7021 gtcggagtag gattatgtaa tcgagtgacc acttcattcg ctaaggcaat ataatcacgg
     7081 ctcgccgaac atttccggtc atattgcaga accgtatctc gctgtaattc agccttttga
     7141 gccgcggtcg acatggagat cgtcgtggac aagcaatcgc ctttaaagcg atcttgcacc
     7201 atctgaaaca ccgcttggca cacgttccga cgagaatcat gtttggtcaa caacacccca
     7261 aggatcgcta aatccggttg cacactgttc ctcaccgtgg cgatgatatt gagtaaatca
     7321 tccaacccgt cgaaggaaaa acctgagccg ctttccatcg gcacaatcac aaaatcactc
     7381 gcggccaacg cattcatggt aatcaacgac aacgacggcg gcgtatcgaa cagcacaatg
     7441 tcaaaatccg tttctttccg aacctttccc ttcaatacgc cgatgggaat cggatacaac
     7501 gagggttcgc gtagccgatt ttccacggac gccaacctaa tattgccccg aatcaaggac
     7561 acattgggca acttcgtggc cgtgaggcag tcatcaagtt taatgtcggg attcaggatg
     7621 agctccgtca cacctgggtt cgcttctggt tgcatctgga gcaatttggt cgcgttcgcc
     7681 tgggcgtcac aatccaaaaa taacacctta taattcatcc tccggagggc gtcggccaga
     7741 ttaatggccg tcgaggtctt gccgcagccg cctttttgat tcgcaatgct gattcgtatc
     7801 gccataaatc ccccttagtc ctgcacccgc aaagccgtta gagaaacatc acacatcaat
     7861 ccgttacaca ctcgatgccg tcgcctcttg gcgcatccgt tcccgaatga gttccagata
     7921 gcgttgttcg cgtttcgcct gttgaatcat cggaatatct ctctccccca ccgcttcaat
     7981 ctctcgcgtg atctcagact gcttctcagg agacaaccgt aaaaagtaca gctcacaccg
     8041 ctgtcgctcc actcgattgc ccgcagcccg taactcctcg gcctcagttt tcagccggcg
     8101 ttcttcttcc tccaacatcc ggacggcccg actcttccat ccagggggcg gattgatttc
     8161 cattttcttt aagcagccaa ataaaaatgc caccggatcc gcaatcggct tcggggacgg
     8221 ttttttctgt tcaatgaccg cgatgacttt atcgataaaa tcttgaagat agcccaaaga
     8281 aggcatctgt tggacaaacg aaagcagtga acgacgatcg agttcttcaa acggtgcaga
     8341 cagtattaac ttatccacag gcacctcttg tagtagttct tgatgagata aagaattact
     8401 actactactg tgcggatgca gagagtcttg cagagagtgc gaggcgaagg tccgagcacg
     8461 tgttttaagc gctggttgcg ccaccaatag cgaggggaag gcttccccga aactataaat
     8521 ggaccctccc gaactatact gcaacagcga ggtccgaact aagagcccgt tatcgatcat
     8581 ttggagcacc acccgccgca ctgaatcctc atgtttgcct aatgttttag ccaattctgg
     8641 ataggacacc ggacgagtcc tggtcggagc agtagtgtct cgatacgcat gcagcgccca
     8701 ggcaacactg acccaagttc ggggagactg aagaagccga ggggtacgtg atccagctcc
     8761 ccggacaacc gactgatccg attcgcaagg aagtgaggcc gccatcccat cggacgggtc
     8821 tttttgcaga gagtcctgcg gactgtgtac agtctgtaga ctgcttgaag cacagtctgt
     8881 acggacggaa tccgctaaaa cacagtctgt attttcccca agactctctg cacatacaga
     8941 ctgtattgtt gattcgtttg cggccacccc cccgtgaggg gtcaaagacg caagactcgc
     9001 tgcttccgat accggaagcg ccttcggcac ttcttctatt aaggtcattg tagagggagt
     9061 agcactgagg accggacttg gagatggagc tgaaccggtt aagcctagca gttcaagggg
     9121 atcaactgga ggactcccag atgtcatttc ttcaaggcgc ttgccgacaa ggcctttcag
     9181 aaaacttctt tcgtggcttt ccacgcgcgg ttttgcgccc accagcgccc ccatcggaaa
     9241 gaaccatccg gatcaatcgt gggttcacga atcgatcatg atggaaagca gaccccaacc
     9301 atcgcggagg atgagtacca caaccatatg actctgtcaa catgagaagc aatgtccctg
     9361 ttaccatggc ccatcctatc atggttcata cgcaatacca ccgcatcaac agaggcgata
     9421 agcagtcagc tatggcgggt gatagagaag aggctaggta agttcacctt ccaacataca
     9481 ggtcagtgtc atggaagcac agagaaagca cgttgggagc taaaatggtg ccattgcgga
     9541 gcttccacca agtactctag gactatctgg tgatgcccct ccgtaagaat gcttgaagag
     9601 tgttcggatc tatcatcgaa gaatcactac gacctgtcga agcgtgacaa gaattcagac
     9661 tgtattgctt agcagactgt accgcgatgc agatagtgtg acgatacgct cataatacag
     9721 actgaatatt catacagact gtattgatag cctcaatcat cgggccagtt cggtctccat
     9781 gttgtgacca tcgatgacgc caccacttct atgacgcatg agtcaccgct agctgtatta
     9841 tgtaacagac tgtaatcatg cacagactgc ataacgttac agtctgtata gtattttgta
     9901 taaatgcaga tagtcttgca ggtagttaac taatttaact atcagaaata ttgagatata
     9961 tataattata tatacagact gtaatgcaga tagtacagac tgaatactag aacagactgt
    10021 atgtaactac agactgtatt atatattgaa gcataaaaat aaaaagagtt gatggccttg
    10081 atcctatgca cacgatggat cttcattgtg tgatgataca ggctgaaatg ttatacagtc
    10141 tgtatgattg tgcagatagt gatattgcct gatgcagaca agttgaaaga atcgtctaga
    10201 cggcgaactg agaatcccga gtaggggagg ggaggagtga aaaatgatgg gccgtcgggt
    10261 tacaccgccg gcgtcgagaa ccattccgga tgtttggctt ccaactgacg gcgatagggg
    10321 gaatggatca aaaattgcca catcacgagc ttcttcatca actcgtagtt ccagggaata
    10381 cccaaatcgc gcagactcca cgacacctca ctgtgtaatt gtttccgctc ggcccccgtc
    10441 aacagcggcg tcaggcgctg atattccgcg agatcctccg catcgaactg atcgacgtag
    10501 cagcgaatcg tctccagggg cgccgtctga tccagcgtcg gacggggagg gggcgggagc
    10561 caatacactt catacatcgc cgggtttatg gaggtcgcag ggcggacgat ttgtaccagc
    10621 ttgtcctcaa tcagcgaggg gatatatttt ttgatcgtat cccacgcgag ccctgtccat
    10681 gcactcatct ggacccggga ggcttcgatc gcctcctgtt gctgtccatg actccaccgg
    10741 agcagttgca gatagagctg ttgttttccg ggagacatcg cgcggtacgt cggatgatcc
    10801 agcgtctgga gcagcttcgt gagaaagcgg aggtggctcg cctcggcctc cgcctcggga
    10861 ggcagaaacg ccgcgagctc cttgtcctcg tcccaggaat tcggttccgt catcacgcgc
    10921 tctctgattt gacccgcctc gcccgacacc tgtccgtgtc acaaacgtga gttccaccaa
    10981 tcacttacgt gcgacccagg accacgcgta gagacgggat aaaacagacc ccagtatgag
    11041 ggctaattta tccccctgtc aagtccggtc aacatgtctg aactacggca aattcaagat
    11101 ttgtggaagt tcagttgacc ggagtcgagc aagggagtaa tctccgtttt catgacacat
    11161 cgacccccaa caacagatcg tctcatggtc cctgcgactc gtctcgtccg acccctcaat
    11221 cgtctccggg cccgccatgt ctgaacgccc ccctgaggct tccctcagcg acgcgtctga
    11281 cgacatcccc tcagcgttgg tagagggagc ccccatgccg atcctcccgg ccaaacaggc
    11341 ggcgaagccc tgggccctcc accggagccc ccgccggatc aagaaagacg atgtcccgct
    11401 cctgcgctgg ccagaccagg ccccgctggg ctcgccggcg gccgatgccc atatccacac
    11461 catcgtcgag agctttctag cctgctttcg caccccgacc accatcgtct cctatcgcaa
    11521 agccttgcgc gggttcttcg actggtgggg tcgtgatgat caggctgatc cactgaccgt
    11581 ccccctcctg cgcgcctatc agacctatct catccacacc gttgaacctc gtacggtgaa
    11641 cttttacctc gccacgctcc gtagcttctg tggctggatg gtcgaccaag gcgagttccc
    11701 ctggaatccc atggacgaaa tccggagcct gcgcatcccc aaaggctttg tccgggatag
    11761 tttgagtgaa gacgagatcc gtcaactcct ccagctcatg cccagaggcg gggccacgaa
    11821 gcctgcacag gaattggcgc tcagaaacta cgccatggcc gtcctctggt gtgccgtcgg
    11881 cctccgcagt atcgagctcg cgcgggcgca gcggcggcac ctcgatttcg atcaagggca
    11941 ccatctgttg aagattcatg gaaagggcga cgatatggcc ggcaaaccgg tcgtgctgga
    12001 accctgggtc atggtcccac tcaccgcgta tctcgagttt catgaccagt ggcaccaagc
    12061 agatgctgcc gggcatgccc ggcccgccca cgaccaccac ccactcttct gttggacacg
    12121 cggccccggc gcctatcgat cccgcaagca caatccggcc ctcacccgga gctgtcggcc
    12181 gattggggtc cgggtcattc aagacatgat gcgggacgca atgggacagg ccggactact
    12241 gaatcacacg gtcggcgaca cccagaacgt ccgacggatt accccccact cccttcggca
    12301 ttcagccgcc acgatcgccc tggagcgcgg cgccccgatc caccaagtcc aggatatgct
    12361 tcggcatgcc gatatccaga ccaccatgct ctacacgcac aataaggaac ggattaccca
    12421 cgcggccgag caccggatcc cggactttac aacaccacca gaccaagcga taaatactga
    12481 ttaattgatc gatggaaaag aggacttggc gacacagaag tggccccatt cattcggaca
    12541 gaatgtttca attcttaatc cggttcatgc ctgttcattc acaagaaaca agaagataaa
    12601 gtcggaacgc agtaggactc gatgaaaaaa cctggctgga aaaaatggaa aaaagcagca
    12661 gtagtacttc tgttcgccgg agcaggtctt ttcacctatt cacactacct tcctgaccct
    12721 caacagcctc ccgacccact atgtgaggtg acagcaagcc aaataggacc tgaatgccct
    12781 caagccacgt ctatagagga tagaatccac aacatagaaa acgcgctgat cgaaatcgtt
    12841 gcagatcttc aacagctcag tgatcatctc tgtccccctg acgaacatac agaagaagat
    12901 ccgatcaaga agcaattgga ggcgctcaaa cacaacctca taacctctct ttatccatca
    12961 gacaccaccc attccgatga agtcattatc cgggtcatcc aatcacggtt ggagaggctg
    13021 cacagttata acgacaaagt cacgcatcag attctagaga gcgggacaat acgacccgca
    13081 tccaaggcag aggtcgagag cctcgtcgag cgtctcgaca cacggctgaa acagctcgtg
    13141 gacgaattca cccacccgcc tgatttctcg caccaccacg cgacactcga aacatttcat
    13201 gaaactatgg aagggctgat agaagtggtc gaactgctga aaaatctctg ttagattgaa
    13261 gcttcgtcat gccttggcca taggcagctc cgcccaattg gtcgtgtagg cgggtgaacg
    13321 gtggtgacac tgcgtctgcc aggcttgcgt caatccattc gatgcgtagt ccagcgtccc
    13381 ggctccccag cgatcattga tggcatccaa ggccgccatc aaccgtttcg acttgggacg
    13441 atcctgctgg tcgaacagat ccggctgcac ctggccggct ggcacgaggg cggtgcacat
    13501 cacccccgct ttcttatagc gatagccgtc ccgatagatg cggcgcagtc ccttcaaggc
    13561 cgctctgatg agttccgagg tcgaatccgt gcccaccggc agttcacagg tcaccgagtt
    13621 gcgatactgc ggctcctccg tgaaggcgtt cgtcatgatg aagacggtca gcaccgtcgc
    13681 cgccaatcct tcccctcgca gcttctctgc cgcacgagag acataggacg acacggcctc
    13741 ttccatctcg gcgagtgtat tgacggatcg gccaaaggcc cgtgagcagg taatcccttg
    13801 cttgggcggg ggacagtcct caagcgcgag gcaggactgt cctctgagtt ccgcgaccac
    13861 ccgcattccg accacgccca tatgcttcct gatccaaggc tcgtccgcct cgcgcaactg
    13921 cagggccgtc gtgatcccgc gctgcatgag caggcgcgca gagttgggcc cgatccccca
    13981 gacgtctccc accgccactc cattcaagag ggcctctcga tccggacagg ccagcaggtc
    14041 aaacaccccg tccgtatccg cggtccgttt cgcaatccgg ttggccagct tggcgagcgt
    14101 ctttgtttcg gcaatgccga ccgagacggg aaggcccgtc cattgcttca ctgtcttgcg
    14161 gatcgtgcgc ccatagtccg tcaggttgcg ccgttcgaac ccagccaggc ttaagaaggc
    14221 ttcatcgatg gaatagattt cgagatccgg gctgaactgt tccaaggtct ccatcacgcg
    14281 ctgcgacaga tcgccataga gccggtagtt cgaggagaag acctgcacat gatgggcctg
    14341 gagcaggggc cgaatctgga actccgggac gcccattgta atcccgagcg ccttcgcttc
    14401 attggaacgg gccaccacac agccgtcatt attggacagc acgacaatgg gttgaccggc
    14461 taatgttgga ttaaagagcc gttcgcagga agcatagaaa ttattgcaat cgacgagggc
    14521 aaagacggaa ggcatcggac cctcagaggg catgaatgac gttggtgacg accccgaaga
    14581 tctcaaaggt ctgttgggtc agaatctcaa tcggttgata gtctgtgttg gccgggagca
    14641 ggcgcaggat ctcgcctcgc ttgaagagcc gcttcaccgt cagctcgcca tcgagcgccg
    14701 ccacgatcac gttcccatcc accgcgtcga gcgatcgatc caccaccagc agatcgccgg
    14761 aatggatgcc agccccgatc atggagtcgc cggtcacgcg cacaaagaaa gtggcagccg
    14821 ggtgcttgat cagatgacgg ttgagatcga gcttgccctc gatatagtcg tcagccgggg
    14881 agggaaagcc agccggcaag cgccccagaa agacggggag cgcatagcgc gtcgagagat
    14941 cgggggcgta gacggcatcc acattcatcg gcatcgaaga cctcacaaca aagaggccgt
    15001 agtgtagtcc tacgccggaa gcggttcaag gcagtccgga gcctcatgcc gcgggttgtt
    15061 cacgagcgta ctgacggcgt aggcctggag atcttcactc ggatagggac gaagcagggc
    15121 tctcagcggt tccgcctgct ggaagctggg gtcgagccac tggtcgtagg aagtggggga
    15181 caggatcacc ggcattcggt tgtgaatggg gcgcagcaac tcgttcggct ccgttgtgag
    15241 aatcgtgcag gattcgatgg ctgcgccttc cggaggttgc cactgttccc acaagccagc
    15301 aaaggcaaag ggatgggaac tcttcaagcc gatccacatc ggctgcttcg cctttccccg
    15361 gacctgccac tcatagaacc ccgtcgcaat caccagacag cgccgggcct tgaaggcagc
    15421 acggaacgaa ggtttctccg ccaccgtctc cgctttggca ttgatgcatt gatgtccgat
    15481 cttcgggtcc ttggcccagg aggggatcag gccccagcgc aactccacta actgacgggc
    15541 tgtcgtccca ggctcgatcc ggatcgccgc caccggctgt gagggggcaa tgttgtagcg
    15601 gggcgtgaac aggggaggga ccgcaacgcc aaactcttcc gcgattaccg ctggcgaggc
    15661 tgtttgggta aagcgtccac acatgggatt caaccttatc atggaagctt ctggatggtt
    15721 tgatgaatca agtagcgagt caggtcaatg gtgagtctgt gtctgtgagt atgttgatca
    15781 gatcgagtca ttgataagtg ggtgaaaaat aagcaattcc cctctcacgg tggggtgctt
    15841 tctttaaggg aaaggggtat tctgcgctcc taatgttgac ggcctaccac gcaaagtact
    15901 tcgcccacga actcaccaaa cgttgtccgt cagacagtat cgaaaagctt gcaagtgcgt
    15961 tagtggatgc gcaggtagac cttaatccac accaaattga agctgcgctc tttgcattcc
    16021 gatcgccgct ctcgaaaggc gctcttcttg ccgatgaggt cggtcttgga aaaactatcg
    16081 aggcagggct cgtcctctcg cagaagtggg ctgagcggaa gcgccgcgtc ctggtcatca
    16141 cgccttcgaa tctccgcaag cagtggcacc aggagcttca ggagaagttt ttcttgcctt
    16201 gctgcatttt ggaaaccaag tcctacactg ccaccgtgcg tcaggggcat gtccgtccgt
    16261 tcgagtgcga cgacattatc atttgctcct accagttcgc ccgttccaag gcggctgaga
    16321 tcagtcttat gcgttgggac ctggttgtta tcgacgaagc tcaccgcttg cggaacgtgt
    16381 ataagccgac caatgttatc gccaatacaa taaagcaggc gctcaaagac gcacccaagc
    16441 ttcttcttac tgccacgccg ttgcagaatt cgttgctcga attgtatggg ctggtcagtg
    16501 tggtggacga ccgcatcttc ggcgatctca aaagctttcg agagcaattc gccaatctgc
    16561 acaacaagcc gaccttcgac atcctgaaag cccgtctgca gccgatctgc aagcggacgc
    16621 ttcgccgtca ggtgttgccc tatgtgcgct ataccaagcg cttgcccatg atgcagcctt
    16681 ttacgccggc ggaaaatgaa gatcggctct atcatctcgt gtccgactat ctccgtcggg
    16741 acaatctgca ggccctcccg tcgagccaac ggtccctgat gacccttgtc ctccggaagc
    16801 ttttggcctc gtcttcgttt gcgatcgccg gagcgcttca ttcaatgagc cagcgacttc
    16861 gcgaacgcct tcgtcttgcc gaagggccga acccccttga agaagccctg gaagaagact
    16921 tcgaagctct ggacgagatg gcggaagagt gggaagaaga ggacgctgtt cctgaagcgc
    16981 tcaccgccgc cgaccgcaaa gcgatccaac aagaaattga cgacctcgaa ggctttcaga
    17041 agctcgccgt gtcgatcacc cacaatgcga aggggcaagc cctcctcacc gctctgcgca
    17101 ctgcctttgg taaggcagaa gaattgggag cggcgcagaa agccattatc ttcacagaat
    17161 ccagacgcac ccaggaatat ctcctccgag tgcttgccga cagtccgtgg aaggaccatg
    17221 tcgtcctctt taacggctcg aacaacgacc tgagatcgaa ggaaatttac caagaatggc
    17281 tccagaacca tcaggggacg gatcgaatca ccggatcgcg ttcggctgac ctgcgctcag
    17341 ctcttgtgga ctacttcaag gaccagggaa aaattatgat cgccacggaa gccggagccg
    17401 aggggatcaa cctccaattc tgctcgctgg tcgtgaatta tgatctgccg tggaatcccc
    17461 agcgcatcga acaacgcatc ggtcgctgcc atcgctacgg acaaaagcac gatgtggtcg
    17521 tggtgaactt cctgaatcag aacaacgaag cggatcagca tgtctttcgc ctgctctccg
    17581 agaaattcaa gttgttcgaa ggcgtcttcg gggccagcga cgaagtgtta ggcgcgattg
    17641 agtccggtgt ggatttcgaa aagcggatcg tcgacattta tcaacggtgc cgcaccccac
    17701 aggaaattaa gacctcgttt gaacagctcc agagcgagct tggcacccaa atcgacgaag
    17761 ccatgactcg tgcccgccag ctcctgttcg aacactttga tgatgaggtg cgggaaaagc
    17821 tgcgagtgag cgacgagagc tcacggctat atctgaatcg atatgaacgt atcctgatgc
    17881 aactcacgcg ctttgaattg cgtgatcatg ccgaatttct ttctgacacc tcgttccgac
    17941 tcaactcctg tcccttccga ggagaaatcc ccctaggact ctatgaactg ccacgtcggt
    18001 ccggggaagc gcatctttat cgattagctc atccactggc ggaacatgtg ctggaacagg
    18061 ggaagaaccg ccagcttgat ccggcagagc tgaccttcga gtatggcaaa catgacggca
    18121 aagtcagcgt ggtcgaacca ctcgtcggta agtccggggt gctgaccctc tcactgttta
    18181 ccgtggaggc gttggatcaa gccgaggact acctcatctc cacgggagtg acggacaacg
    18241 gcgaagtgtt agatgaggaa gccgtccgtc ggctgttctc tctgcccgcc acagttactc
    18301 agcctgtgaa gtcatcgatt gagcatccag cgctcagtac gcgtacagaa gaacgtcagg
    18361 atgccattcg ccggcacatc tcacggcgca atgcggaatt tttcgagatc gaagccgaca
    18421 aactggacgc ctgggccgac gacttgaaag tgggtttaga gcgagagatc aaggaattcg
    18481 accggcaaat caaagaagcc aggagagccg cagtggcagc cctcacgttg gaagagaagc
    18541 tggccggcca aaagcagatc aaggcaatcg aagccgaacg aagcaagcgc cgtagggccc
    18601 tgttcgatgc acaagacgag attgaccgtc ggcgagagca gctgatcagc gagattgaag
    18661 gaaagctgca acaaaaggta aacttccagc aattatttgc cgttcgctgg caagtgcggt
    18721 gaaggctggc cgtgagtcct gacaagtcca ggcatagcaa tacttgggga gatcctaaga
    18781 agaaatctat gagtgaaccg accatcacct gtcccaactg cgagacggaa atcaagctca
    18841 ccaaatcgct gactgcgacg cgggagacca ccggcaagca atatgagctg ctgttggcgt
    18901 gagattgttg aggagcggaa ttgatggcac agacaagccg catcgaatgg accgaggcta
    18961 cgtggaatcc aaccgtgggg tgcacaaaga tttcacctgg ctgtaagcac tgttacgccg
    19021 aagccatggc gaagcggttg cacgccatgg gcacacctgg ttacgaaaat gaattcaggt
    19081 tgacggttct gcctgatagg ctcaacgagc cactgcgtcg caagaaaccg acgatctatt
    19141 ttgtgaattc gatgtcggat ctgttccata agaatgtgtc ggatggttat atcgagcaag
    19201 tttttgatgt aatcgcacgt tgcacacacc acacctttca ggtgttgact aagcgagccg
    19261 aacggttggc ttcgtatttc ctgcgcagaa ccgtaccgaa gaacgcatgg attggggttt
    19321 cagtcgagga tcaaatctgt ggtttgcccc gcattgacgc actaaggcat gttaatgccc
    19381 atattcgttt cttatccgtg gagccactgt tggaagatct tggggaaatc gacttgaccg
    19441 atatgcattg ggtgatagtt gggggcgagt ccggcccaaa cgcgcgtccc atgaagcggg
    19501 aatgggttga gaatatcaag cggcagtgtc aggagcaagg cgtccagttc ttctttaagc
    19561 agtggggagg ctggggtgcc gacggcaagc gtcgctctaa gaaaaaaaat gggcggctac
    19621 tcgatggccg cacttgggat gaaatgccgc ctctctcaac tcgaatataa ggccaaggaa
    19681 tgtctcgcaa gcactacgat tggaaaaacg ggcctgatgt tattcagcag cacagcatcg
    19741 ccaagcatcg cattcttcag gcctacttag ccgcctattt tcagacgttg gtgagttctc
    19801 cgagtcagga tgttcttcga cttactctgg tcgatgggtt cgccggtggt ggtctgtatg
    19861 tacacaatga cacgaaagaa ctagtcaaag gttcgccatt tatttgcctc aacgccacaa
    19921 aggaagcagg ttttctaatc aatagagaac gacggaagcc ggtgaacttg gatgtcagtt
    19981 atttcttcgt ggaggccgat cgtcacgcgc atctgcattt ggagaagaca ttacgcgaag
    20041 aaggttatgg accggagctg aacaatagca tctatctgcg acacgctaag tttcaggacg
    20101 aagcggatag catcatccaa tttatcaagc aaaagagtcc aaggaacgga cggtctattt
    20161 tcgtgctgga ccaatacggg tacaaggatg taccgacgga gttggtccaa aagattttca
    20221 ggaatcttcc aagtgccgaa gtgatcctga catttggtgt ggattctttc ttgaactttg
    20281 ccagcgatac cgggctgaca gccaggttac tggacgagat tggcgtgcca gacctattgc
    20341 aaggaagaac tattgatgag atcaagggct ctgaacggaa ttggcggttg tttattcaaa
    20401 gcggtttata tcggagactg gtgacacgct gcggtgctaa gcactacaca ccatttttca
    20461 ttcgcaacaa aaaggggcat ggcgactact ggctgatcca cctgtcgcag caccatcgcg
    20521 ctcgtgatgt gatgactgaa gtacattggg acaataacaa ttacttcatt cattatggtg
    20581 gtccaggcct agacatgttc catatggttg gttacgaccc ggatcatgac tcggcccaaa
    20641 aagggcagtt tgagctgggg ttcgagtttg atgacgtggc ccgtaacgct agcatcgcgt
    20701 ccctgatgga acaaatcccg cgtcttatct atgccaacaa tgagggtgca agcttcggcg
    20761 tactatttgc gaccacttgt aacgagtcgc ccgcctcggc aaagatttat cgcagcgcta
    20821 tcgagcagtt gatagagcat agggtcgtcg aggtcatcag tcctgatgga gtcagtcggc
    20881 gttccgcaca gcagattcag tcgtcggatc agatcgtcgc gcctggtcaa cgttcgctat
    20941 tccactgata aaccgaatac gaaattgcca aaacagcgaa atgtggttgc ggcaacttat
    21001 gaggggagga gctgattaat gaagctccga tttctctcat cactgctaat ttttgtcagt
    21061 tcctattttc cactggcgct gatcttcata atcaaagacc tggacaacga aacgtttctt
    21121 cccatgcatg ctcttactgc ggcgataatc accgtattgg tgctaatcgc gtgcggcatc
    21181 gtgcttcacg ctgctagaaa gatcgagagt ggcgttcccg tagaggttac aaaggtatct
    21241 aataagtccg gagacatgtt tacgtacaca atcccgtaca tgatttcctt ctacaatttc
    21301 aacctcggcg attggaagac cctacttagc ctcttcgtct tcatgactct gatgttcgcg
    21361 ctgtcctacc gaacacaaaa cgtcttcatt aatccggttc tcgcgttggc tggttacggc
    21421 ttgtacgact gtcagttcag ggatggcagc cgcgaggttc agggcctact gatttccagg
    21481 tacgaatttc agcttggcga tacctgcatt gtcgaacagc tatcgaagtc ccagtacttc
    21541 gtgagcagcg tccaggcaaa ggagaaaaag gatggcagct aagatcgcaa agaagatagc
    21601 ggatctgcgt cagacagact ttaagccttg gacgaccacc ctttggctgg tcaaacggcg
    21661 cctggacgtc cacaaaaaag cacactattc ggtgttgcgt gtcgatgtcg acaagaagct
    21721 acaagacaag ctcaagaagg cggttacaga gcgaatccat ggcgtggatt taaagcttga
    21781 agcgtatgag tttctgacgg acgaccaaga tggcaggcta ttcaccattg aatccgcaga
    21841 aacagacttc atcaagattc aggctgaggt ggacaaggga ctcgaaaaca agaaggtcga
    21901 gaaatacgaa gacctattgg attcgtgggc ctgcgtgatt aagctggaac atcagggcag
    21961 tgttttatac ggtgttcgaa aaataaataa gttcgcacgg gccacgaaag tcaaagcagt
    22021 gtcctatttc atcttcgaaa acaaaaagct agtcgatctc gaaggcaagc aagtgttcac
    22081 gctggacacc catatcgact tctttgctta tgacggcacg atgttcattg ccaacaagaa
    22141 ggagttcgag agcgtgctga acttccgcaa tggcatggaa aagaatagag atgctatttt
    22201 ggaagaattc gtaactttaa aggtggtcgt cgatgtcgaa gtaattcgaa gagttgttgg
    22261 tggaaatctc cacttactaa ggaagatgtc gtcgattcaa aagagcggtt actacaagga
    22321 caagatcttt ttggttgagt tgaccaagaa gaacgtggag aagggctgga atctggaagt
    22381 ggaacatggc gttattgtcg ttgacgaaag caatgtcgag ttggtgttaa aacttctaaa
    22441 caacgaacgt gtcgaatcgc agattaacca ggaagtcttc gatgctgtcg tgaagaagaa
    22501 ggtcggatga tcaaggcgtg ttagtgaaca agaaacaaaa actcgaactt acctggatcg
    22561 ggaaagaaaa ccgaccgaag ctggagccgc gtatcctgct ggaagaccgg gagaagtcct
    22621 gccatgcgcc gcaccgtgtc accgaccgcg acttgttcga taacaggctc atctttggcg
    22681 acaatctgct tgccctcaga gcacttgaac aggagttcgc cggcaagatc aagtgcatct
    22741 tcatcgaccc gccttacaat accggttcag cattcagtca ctatgatgat ggaattgagc
    22801 actcaatatg gctgtcgttg atgcgagatc gtctagagat tatgcggcaa ctgctgaccg
    22861 atgacggttc gctgtggatt accatcgatg acaatgaggc acactatctc aaggtgcttt
    22921 gcgatgagat attcggacga agcaatttcg ttgccacttg cgtttgggaa aaagataagg
    22981 gtggccgcgg tgatgcagat atctcacttt cacacgataa catcctcgtg ttttcaaaaa
    23041 cccgaaccgt ttggtccaac tcacggaatc tcttatcgcg cacagaaact caacttgggc
    23101 gatttaagaa tccagacaac gatccacgtg gtgaatggcg gcagggagat gacggtacgg
    23161 caaaaagtgg aactgaaaaa cagcggtttc ccgtaacgtt gccctctggc agggtagtaa
    23221 cgccgccccc tggaaggtac tgggcttttt cgcctcatac acttgaacaa gctcgtgccg
    23281 aagggcgggc ttatttcggg gttgatgggg atcgattgcc aatcattaag cggtatttaa
    23341 gcgaagtgcg tgaaggtgtt gcgccccgca catggtggtc ggctgatgag gttggcacca
    23401 atcagcaggc gaaacgcgat cacctaaaca agctcttgcc agacattgaa ccgtttgcta
    23461 cgcccaaacc cgaaggactg attcagcgaa tacttcatat tgcatctaat caaggcgact
    23521 gggtgcttga ctcctttgcc ggttcaggca ccaccggagc tgttgcacac aaaatgggac
    23581 gacgttggat catggtggag ctaggcgagc attgtcacac gcacatcatc ccgcggctca
    23641 agaaagtcat tggtggtgag gataagggag gcatcactga gtccgtcggc tggaaaggtg
    23701 gcggcgggtt ccgttactac caccttgcgc catcgttact ggagaaggac aagtggggga
    23761 attgggtcat caacaaggct tacaatcccg ccatgcttgc tcaagcggcc tgcaagctgg
    23821 agggctttgt ctacgagccg agtgatgcgg tctattggca gcagggccat tcaaccgaac
    23881 gagacttcat ctatgtcacg actcagaaca tgagccatga tcagttgcag gcactcagtg
    23941 atgaagttgg cgaagagcgc tcgctcttgg tgatgtgttc tgccttcaga ggcaagcccg
    24001 ataagtaccc caatctcacg atcaagaaga tccccaagac cgtgcttacc cgctgcgaat
    24061 ggggccatga cgattacagc ctgaagatcg agaatctgcc caaggctccg attccgccag
    24121 gtcagatgga gctccttggc gaagaggcga aatcatgaat cgacatgtca atgccatagc
    24181 cggacgcctc agtctgcggc ctccgcaacg tgagtcgctg gcaattctcg accgcgtcac
    24241 ggagattgtg cctcccaaga agggcgcgga cgtcgcagct gcactagctg ctatcaagag
    24301 tgagttccca accgtcaccg actttgagcg ggagtttccc tcactctgct ttgctctcgc
    24361 aaccggtgtc ggtaagacgc ggctcatggg cgctttcatc agctttctgc atctcgcaca
    24421 cggcatcaac cacttctttg tcctggctcc aaacctgacc atttataaca agctgattgc
    24481 cgactttaca ccaaataccc cgaagtacgt ctttacggga atcgccgagt tcgcgaccga
    24541 gccgccgacc attatcacgg gggataatta cgaggccggt gtcgcggctc gcctcacctc
    24601 cttgccgggt tttgggcaag aggtgcacat caacatcttc aatatctcca agatcaactc
    24661 ggaagttcgc ggcgggaaag cgccacggat taagcgcctg tctgaataca tcggccagag
    24721 ctattttgag tatttggcag gccttgatga tcttgtcttg ctcatggacg aatcccaccg
    24781 ctaccgggcg tcagccggtg tgcgggccat caacgaactg aaacctgtgc tcgggctgga
    24841 actcacagct acgccgttcg tggagtccac ccaaggcccg gtgccgttca agaacgtgat
    24901 ctatgactat tcgttggcca aggcgatggc tgatggcttt gtcaaagagc cggcagtcgt
    24961 cacgcagaag aatttcaacc ctagccagtt caatgctgct caactggagc agatcaagct
    25021 ggaagatggg gtgcgccttc acgagaatac caaggtggag ctggaaacct acgcaaggca
    25081 gacagggcaa cgagtggtga agcccttcat gctgattatc gcccgcgaga cgacgcacgc
    25141 gggacagctg atgaaactga ttcagtcacc cgagtttttt gaaggccgat acagggacaa
    25201 ggtgattcag gtggactcca gcaagacggg cgcggaagaa gacgagatgg ttcaacgtct
    25261 tcttgccgtc gaaagcccag atgaaccgac cgagattgtg attcacgtga acatgctgaa
    25321 ggaaggttgg gacgtgacga atctctatac gattgtcccg cttcgcgcag ccaacgcgcg
    25381 aaccctcatt gagcaatcca tcggacgcgg tctccggctt ccctatggca agcgaactgg
    25441 ggtgagcacc gtagatcgcc tgaacattgt ggcacatgac cgtttccaag aaatcatcga
    25501 tgaggccaac aaacccgact ccatgattcg actgacgcag gtgattctcg atccggcgac
    25561 tgatcttcaa aagatgcgaa cggtggtcgc tcaatcaaag atcgaggaac agttgcacgg
    25621 agcctccctg actggtcctg ctgatgggcc gccgcaaact actcccattt ttgcgggaga
    25681 agcggagcga gggattgcac aggcgacgta caagatcatt cagaaatacg agaacctgcc
    25741 ttcctcgaat gacctcttaa agcctgagat tcaggcaaag attgtcgaag aagtaaaaca
    25801 gtccataact ccaagtcagc aggccctacc tggcatggcc actgtcccca gtattgcagc
    25861 ggttgtcgcc aagacggctc aacttgtcgt gcagcagtcg atcgatattc caagaattct
    25921 tgtcgtgccg aagggtgagg tcaccaccgg gtttcattct tttaccttgg attgcgcgaa
    25981 catccactac caaccggtcg aacgggacct gctgatccaa attcttcgca ctcacgaaca
    26041 ggaaacgttg agttgtggtg cgcggatgca gcctgagcaa cggctcgaag attacttggt
    26101 ccgtggactc gttgatcttg atgacatcag ctacgacgaa cactccgacc tgctgtatga
    26161 cctcgccggc caactggtca gacaccttcg ctcatatctc tccgaagagg aggctagaaa
    26221 tgtcctcatc taccatcaga agcagctggc agcgttcgtc catgcacaaa tgcaggcgca
    26281 tcagtgggag aaggccacag gttacgatgt ggtggtcagt aaagggttca cggatctgaa
    26341 gcccagcgcc tatacggcga gagacggtga gccgatccag gactttcgtc aaaccgtcga
    26401 ggatcggagc cggatcggac agatgctctt tggtggattt cagcgaggcc tgtattccgt
    26461 gcaaaagttt gactccgata ccgagcggcg gctggccatt attctagacc gaaacgcaca
    26521 gaaatggttc cgaccgtcct ctgggcaatt ccagatttcc tataagcagg gagtctcgga
    26581 gcatgactat cagcctgatt ttgtagccga aacagcagac tgcatctata tgctcgaacc
    26641 caaagccaag aacgagatga ccacacccga ggttctcgcc aagaaagatg cggctatcat
    26701 ctggtgcacc cgcgccacga cccacgccct cagtaacggc ggcaaaccat ggaaatacgt
    26761 gctcatcccc cacgacatgg tcgatcagaa catgacgctg gcgggattgg tgaagcagtt
    26821 tgcgaccgag tgagagtttc ttatggattt aatttttcgc ctcctgtgcc ggcaataggc
    26881 ttgactcagc cttgtgcttc ttgtggggaa caggttcctt acctgctttc gatctaacga
    26941 tggatcgtct ctccaatcta tgtaaacgca gtacctggtg gggtttgcct ctatcgtcca
    27001 tggaaagcct ccgttcccta ccgtttgtga taccttttca gaagatcaga aatagtctct
    27061 tccaataatt actacccagt ctggttttgc tctgtggcga gaatgcgaaa gagtcggttc
    27121 gtgttcgggt cagagtgtcc gccaagcaat tgcgtacccg tcctctgggg cgggacgcgc
    27181 gaagcgcgta gcggactagc tgagctccgt cctgttcgtg tgccgtccga gcttgttgcg
    27241 ccatctctca ccctccgaca gcggaaagtc gaatagtaga aactgagacc ggagttttcg
    27301 gagactccgg tctcgcgctc ccgttagccc tggatggtca agagggctac gcgtacattg
    27361 gtgccagctt cctgaaagct gccctctggt aaaatctccc aggtctcggc aatcggcttc
    27421 agttgctctt gctgccgggg gccgttggcg cagagggcta ccagtttgcc gccaggcttc
    27481 aagaagtgtc gtgcgtgtag aatgtgtttc acatcgtcgg cagacccaaa aggcggattc
    27541 atcaggatac ggtcgaacgt acccagctcg ccggtacagg taagaaagtc tcccagccga
    27601 atatcaacgg aaccgtcatc ccatcggcgg cgaagttggg tcactaacgc cgcattgatt
    27661 tcaaccgcga cgatctctcc cggcacgtcg gccattgcct caataagatg cccggtgcct
    27721 gcttcaggct ctagccgtcg ttgtcctggt tgaaggtcgg ccttctcgac catctgcgcg
    27781 acaatcgcgg gcggggtcgg gaagagttgc ggggctgaaa cggtctgaac cccggccttc
    27841 aggctgtcct tcatggcgtc atagacggtt ggcgcgggcg gctgatgctg ccggatagtt
    27901 ggcgcggtgc cctcgcgttc tggcaaggct ggcggggtag ccttcgcggg tgggtctttc
    27961 cggggcgcgt cggtcacgta aaccggaacg ctgctccatg cgtccttgcc tcgcgtctgc
    28021 tggactcggt gcgctccgtg cgtgtccgtg gctttaatga ctcccgtttt cggagagtct
    28081 gaatacatgc gccaacggtc ggccttcatt tcctctttcg tcatgtgcgt gaacccttcg
    28141 cctgggtagt tgcacagcgg gggcaatttg gttgccgcct tcactttctc ggtatctccg
    28201 gcttggggta gcttgtattc tttgatctcg tcgaagggga tcgttccggc aaagtgtcca
    28261 ataacggata cgctgcccgc gttgactttc gtaataatga accatttccc acggcggaga
    28321 atctgtcctc cgatctcgaa tttctgacga tcggagacga gccccccggc ctccgcaagc
    28381 atcgccgttt catactcaag ccggttggca tagtgggtca accaacgatc gcaacgggca
    28441 ataatgccag ggtagagtgt cttcgcggcc tcgagtacgt cttccagggt ccgcggggcg
    28501 tagagtgtcg ggctcgatcc ggtcaaggca tcataggccg ttggtgtgta ttgccattcg
    28561 ggccggtcgc cgtcttttcg cgggagatgg aaggaggaat cattcgcaac ggcgagggct
    28621 tgctcatggg ttaagccttc agggctccac aacttcaaga atttttcggc ttcgtccttc
    28681 gtgcgttgcg tcttgcgctg gtccgcttca atccctttga ttcgtcgcgc gcggactgcg
    28741 ggaagttcct tgtatttagc atggtgaatc gcgccagcgg cgcggctttt ccagtattgc
    28801 gacgtttccc acatatctac ggcttttctc ataccgttct caatgcgtgt ggcgtctcgg
    28861 cgggcgtggc gctcgctgtg atggccgatc aggataggct gaccgagcgg gatattgtcg
    28921 gcaatggctg cgaccgcctt ccgtgctcgc tcggcgtcct tggcgcggtt ctcctgatag
    28981 ccttcaaagc gttcggcgcg ttcctccgct cgatcaacga ggctcgcgtc ctcgtcgtcg
    29041 atctccccgg ctaactcgat gagcaagtcc tcgcgctctg gtgtccacat gggcgccaca
    29101 aagaggcgtt gtttcggtgc ccagataaag ccagcggctt tcacacgggc ataggtttcg
    29161 ttatcgagtc gggaaacgct gtagagcctc aatttattgt catcagggga ataggtggcg
    29221 gtcatcatgt gcgtgtctcc tccttggttg tctgaatgtt gcgtggtggt catggtgtcc
    29281 tttctgttgg ctcctctcac gaggagttag agcactccgt tatctgcgaa cgagtagtac
    29341 cgatccgcgt ctccaatcac gagatggtca agaattgaaa tgcccatcag ctccccacat
    29401 tcccgcagac gtttcgtcaa gagacgatcc tcttgactgg gcgttgggtc gccggagggg
    29461 tggttatggg caatgatcat ggcagcggca ttgcagagca cagcggcctt gaagacttcc
    29521 cgtggatgga caagggataa ggaaagggtc ccgactgaga ttgaatgcac cgcgttaatt
    29581 ttgtgtttgg catcgagaat cactgcaagc atttcttcgc ggtcattgtc tccaatgatg
    29641 gaccggcaga gcgcggcggc gctgtgcgaa tcagagagtg tgcggcggtc cgtcgtgata
    29701 gagccgtctc ggatcatgct gagttttacg actgggattc ggtagttcat ggcgtccttt
    29761 ctgttggctc ctctcacacg gctaggagtg gagagcagag cgtggtgagc ggtgtaggcg
    29821 aagtgccctc tattaaaaaa acactcaaaa gcgacccccc caccacacac agggggttgc
    29881 gagaatcttg gttgcggtgg ctgtgagtcc tgcgagcgcc tgactgctaa ggcaaagctt
    29941 gctccgctca cagaacccgc acccacgggc ttatccgatg acgaggtgta ggtgatggat
    30001 ggtgcgaaga gagagacaga cgcggctcgt gtgggcaacc ggcctcctcg gaaggtgtcc
    30061 cgttgtccac aagccccgcg cggagagatg aagaagcccg tggatgtgtg gacggctcgg
    30121 aacccgccca cagatccacc ggcacggcgg cggttttttc ctctctctct atgtgtttcg
    30181 gaaatcgcta gatcgagggg cctgagctac ggagcgtcgg tatgaattct gagggagggg
    30241 gggaacagat taaacccgat gggacacgat cgttctgccg cagtttggcc acaagtcgca
    30301 cgcctcagga ggttggctct gaaacagagg ggaagttacc attctgaaaa gctgccgatt
    30361 atttgagtcg gtgtccacaa aacctgcaca catgtcgtcc cgtaaattct cgaactcatg
    30421 tgaccaagaa ctcgacttcc accgccccta aatcagatat taaaaaatta tctggtgata
    30481 aacgtattgc tgctcaacac ttctgcgggg catgctggct gtgctttgga taacgcgagc
    30541 aagttatgat tgagccacaa aagtcgaact aggatctttg gcctgtttcg gcgtaaaatt
    30601 atagaattag atcggcctaa atctggaaaa ccaagaaatt gtccggagca cgcaacctac
    30661 acaaatcgct caagtctgag attcttggca caagtaccgc ttcgggggct gaacttgctc
    30721 tggttaaact cgcatggggc aaggaggatg tggcgttcac ccttcacgtg gtcccgaaca
    30781 ctttttcaac ctacggcatt cagattcccg acctactcag ctactcaggt ttctcgcggc
    30841 agccgtgctc tttcttaaca cgcgagtgct atgtcaaaga ggtcaatgca gatgttaatt
    30901 tgaatgcctt tggaaaagca cttggagagg cctatcagca actgtctcaa gcgcagcgtc
    30961 acctggagcg ttgcggattt atactcgatc agcccgaggg ttggggctac ttttttgggc
    31021 agcaatcggg tggtcgttca cgcagcgact attatggcga tgggcatatt tcagccatac
    31081 ccccacaagt aatgaaaacg gctgaggacg acgtatttca ctttgtcttt acttggatca
    31141 agaacagcga atctgaaaaa ggctggacca tccattatcg cccgaagcac tcacccttgt
    31201 ctgttgaact acagggcgta ttccatttct tgggaatcga cgagtttcgg gaatgccctc
    31261 agtttgagtt cgagtcgtgt tcttggcggc acatcgagtt tcgcgaaggc ggcgatcaca
    31321 tatttgaaaa cggaaatgca agcgttgctc acggctggtt cgactctcat caattaaatt
    31381 tttcccctgg tatcaaagca ctccttaccg cacaagcgac gatggaagaa gtgggcatgg
    31441 gcttcctgcc ctcctttgcg cagccagaga tgcggcgaac gcaggagatc actcgacata
    31501 tcacctcacc aagcaccatg gctcctaagc cagcgtccag cccaatgttt gacgttgcaa
    31561 tttcattcgc gggtaccgaa aggaaacttg ctcaggagtt ggcaacaagg gtcaagacgg
    31621 cgggtttcca ggttttttac gatgagtttt tcccggagga actctggggt aagaacctcg
    31681 gagagttctt ccatgagatt tacagtaaac gctccagatt ttgcgtaatc ttcgtctcag
    31741 ccgagtactt agagagagaa tggacagttc atgagcggag aagtgcccaa gagcgtatgt
    31801 tgaaagataa gggcaatgag tacattcttc ccatcaaagt agatgatgtg gagttacccg
    31861 gcatcccttc gactattggt tatctgccga taagcaccgg catcgataaa attgccgatg
    31921 cccttattag taaacttaag aataaacgat gaaggggtca gctcaactag atagctgacc
    31981 tggcgtgtgc ggctgatttc gagcgtaaaa cctgaacaac tatgtcagac cttccccaat
    32041 caccagacga caaggccata atcgagagac tgcgctccga gctagcgaag tcagaaccaa
    32101 ctcgacacaa acgcattctt gaaaagtttg ttctggcagc tcttggtagc attccatgga
    32161 ttggcggctt tcttagcgcc gctgcaacct acaaaactga agaaggatca ctgaaacaag
    32221 actcactaca aactcagtgg ctagaggaac atcaacagaa aataggcctg cttcgagagg
    32281 ctttggaaga gatacgtcag cgctttgaaa aacttggagc tagtattgaa gaccggatac
    32341 aaagcgaagc gtacctggcg ctcgttcgaa agagctttcg cgcatgggac gaggcggaca
    32401 ccaaggagaa gcgtcggtac attgcaaact tagtcacaaa ctcagctggt acgcgcgtgt
    32461 gctccgatga tgttgttcga ctattcttag attggctaga gctttaccac gaaatccatt
    32521 ttgcggtaat ccgtgagatc tttgagaacc cgggttccac gcggtttgga atttggtcag
    32581 acctttacgg agatactcct cgcgaggatt ctgcagaggc tgacttgttt aaactcctga
    32641 ttagagacct gagcacaggt ggagtgatca gacaagagcg tgatgtaaat caattaggcc
    32701 aattcgttag gaagacacca cagcgcgctc gtcgtgggac agcacctacg acaatagaat
    32761 ctgctttcga ggattcaaag ccgtatgttc tcaccgaact cggcaagcaa tttgtccact
    32821 atacaatgag tgaagttgta agccgcattg atggcccaaa tgtgcgccct agctgaaacc
    32881 ggtctagcaa ttgctcgcag cggttacctg tctccgccag gtcgtaaatg gtcgtatagc
    32941 agagctattt tctgctcctt cgctatcact caatctcact caacagattt acttccaaag
    33001 aatgaagata attacttcaa cggacaaatc tcgattctat taggctacac ggcgtgtcgg
    33061 tttggccttg atcaccccat tcaagttgat cctctgatag cggtcggtcg tgaacttcgg
    33121 gtttgggtcg aggactgcca tcggccaggt gcggaagttc agtgccgaga tcgcaccgcc
    33181 ataaagcggt cctcagccct gtcgcattaa cagcgtgcgc ctaagctgcg gcgcgctgaa
    33241 gaagaagaaa aaaacagtca ttccttagtt tacgcgcgcc gtcagcttca ggcgcatgtt
    33301 agtccgctcg ctcaagtttt ttgttgaacc gctcccacgc agacagagca cgagtcatga
    33361 gctgtagtgc gtcgccaatc tcaaggccat tcgaatcgag aatcggcagc tcgaacgtga
    33421 cgaggtctgg gcgagctaca tccgtctcca cttcaaaggc gcgaaacgtg acactgcgca
    33481 tagcctcttg agggatctcg taccgcagcc tacccaattt cggccacttg ccagagcggc
    33541 tgttcgtgag tttgccatgc ttcgcagcgt tggcaatgtc cgcgctgagg gcgaggctgg
    33601 gatccccaga tgcgtaagcc tcgacgtctt ggggatccct ccccaaagtc gcagcacgtc
    33661 ccttgaggta gtccttgaga ctccaaagcc agattgccag tgtcaaaact tcgtgttcaa
    33721 gctggtccgg ggattttatg ccattgatga tgtaccggtg ggtttcaccg tctggaaatg
    33781 tgtaggacca cgcgcagcca ctccccccga aagcggagac gactttgctg aagtgagcag
    33841 ctctatcgaa aagtagagca acagaggctg cgtccaattc ggactcctga gaatcgcgag
    33901 cggcctaaca atgtttaggc ggatccgtat aagaaccgga tctgccatgt tctatccgcg
    33961 taacacacct tcaatgatcg tgaacaatat cccatcatcg acggtggagc aaatggttgc
    34021 ttttcaattc ggtgatcgac cggtttgggt
//
LOCUS       DAORRQ010000045        32831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_79_length_32831_cov_21.951794, whole genome shotgun sequence.
ACCESSION   DAORRQ010000045 DAORRQ010000000
VERSION     DAORRQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 32831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32831
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79_length_32831_cov_21.951794"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK224_14060"
     CDS             <1..454
                     /locus_tag="PK224_14060"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRB16916.1"
                     /translation="NIIPGVKHVVAISSGKGGVGKSTVSVNLAVALALTGAKVGLLDA
                     DIYGPNIPMMMGVEKTPEQKDGKIAPAESHGVKLISMGFFVPEDTAVVWRGPMVHTAI
                     QQLFRDVLWGAVAFLLLGRAPGAGGGGGRRGWPGPPGAGRAGATWIIC"
     gene            439..933
                     /locus_tag="PK224_14065"
     CDS             439..933
                     /locus_tag="PK224_14065"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRB16917.1"
                     /translation="MDYLLIDLPPGTGDAQLTLTQLVSLSGAVTVTTPQEVALHDVRK
                     GMMMFQKVNVPLLGIVENMSFFLCGHCGERTEIFSHGGGERAAEKLGIPFLGRVPIDP
                     AIRAGGDTGNPIVVAKPDSPQAQAFREIAAKLAAALQTGEAGAAKSRIASLLDKIKRP
                     ATGN"
     gene            978..1283
                     /locus_tag="PK224_14070"
     CDS             978..1283
                     /locus_tag="PK224_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRB16918.1"
                     /translation="MGELIRIAGTADVKPGAGMVAEVNGKAIAVFNVDGAFYAIDNTC
                     VHRGGPLGEGDVEGDVVSCPWHNWQFNVKTGTCVNNPSAKVTAYEVTVEGTDIKVRL"
     gene            1362..1730
                     /locus_tag="PK224_14075"
     CDS             1362..1730
                     /locus_tag="PK224_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16919.1"
                     /translation="MADNTLTPKHIEIAETFVRLYVFLTQYIDRCEDEAARKEYPEAE
                     LQKHLGETRAKMMEILKVNPVVKGKVEKECERVLALGSKCLMGGTEKVAALDVLGAER
                     VILKNKTIALSDLLAVYRAL"
     gene            1738..2091
                     /locus_tag="PK224_14080"
     CDS             1738..2091
                     /locus_tag="PK224_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16920.1"
                     /translation="MSTPAGEKHQLAGFDARERGFNRPVEFVRTASGYRATLRYETTH
                     IVTPEAPSAGEALQQLITTLQQEGYTQLRSQQSYRDGLYLGSQELWVEYPDRQAAPQV
                     GLLGLLKKWLGRSPG"
     gene            2229..2675
                     /locus_tag="PK224_14085"
     CDS             2229..2675
                     /locus_tag="PK224_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16921.1"
                     /translation="MRWVHVLLLTIIVLLPAGKATAKVYRCQGASGSTVLTDQPKGKT
                     GCVAIDTATPPLPGGFTPPVEPSVPAQVELPANAIPPSISAAPMVPHQPGPGDPALSP
                     AVAKDAPEKAPPEAQRCSPRVNPLNPFAGMNCSPASGEPPAENKNQ"
     gene            2774..3148
                     /locus_tag="PK224_14090"
     CDS             2774..3148
                     /locus_tag="PK224_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16922.1"
                     /translation="MSAFSAGFLSRRAQDFRAGLRHAFAVRPEQAAFTIEDLALLERV
                     ADAVVSRGMAAPATLFLDSMGPMNFLGSQALHFLAPVVECVLTGTEIAQVARLLERRD
                     SIHRLTALIEAKAAAPQGAPAR"
     gene            3193..>4275
                     /locus_tag="PK224_14095"
     CDS             3193..>4275
                     /locus_tag="PK224_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRB16923.1"
                     /translation="MNVIVATDCGSTTTKAILIEKVGEEYRQTYRGEAPTTVEAPFED
                     VTRGVLNAIAEIEELSGRTILDGDRIITPNQAAQGDPKRGVDIYVSTSSAGGGLQMMV
                     TGVVQNMTGESAQRAALGAGAIVIDVLASNDGRLPYEKIERIRTMRPDMILMSGGTDG
                     GAVTHVVEMAEYIAAADPRPRFGSTYRLPLVYAGNTDARPQIKTILGEKTALDFADNI
                     RPVLERENLAPARNKIHDLFLEHVMQQAPGYKKLIEMAGAPIMPTPAAVGVIMETIAK
                     REGINLIGVDIGGATTDVFSVFNGLFNRTVSANLGMSYSISNVLAEAGLDNIMRWVPF
                     TIDEQTLRNRIKNKMVRPTTIPQSLDE"
     assembly_gap    4276..4285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4286..5005
                     /locus_tag="PK224_14100"
     CDS             <4286..5005
                     /locus_tag="PK224_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRB16924.1"
                     /translation="LQIEHAIAREALRLALIHHKSLATGLKGIQQERTISDVFEQRTS
                     GKTLIDLLKLDLIVGSGGILSHAPRRIQSMLMMVDAYEPLGVTTLSVDSIFMMPHLGV
                     LSTVNEQAATDVFVRDCMVYLGTCIAPIGQGKDGERCADYEIALPDGRIERGQLAFGD
                     LKLFALAREQQATVTMQPAKQIDLGNGPGQSFTKTVKGGVVGLMLDGRGRPLQLPGEQ
                     AARVAMLTRWYQAVGLYPVGS"
     gene            5320..>6197
                     /locus_tag="PK224_14105"
     CDS             5320..>6197
                     /locus_tag="PK224_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16925.1"
                     /translation="MAHSYTPGLTVTEQTTVRRRRILPLPGTVLVKVGDVVRADTAVA
                     RAELPGKVVPLNLANQLGIAPDEINDYLIKKEKDSVQKDDVLAENKPFIKWFKTEIRS
                     PITGKVESVSTITGQILLREPPRILELRGYIDGSIVEVHPEQGVTVETSCSLVQGIFG
                     IGGETSGELVMGVTAPDQPLTPELLTPAMKGKIVIGGAFLSAATMTRAKELGVIGLVV
                     GGIHDKDLRALLGYDLGVAITGTEQVGFTLILTEGFGTIPMAPKTFALLSAHAGHKAS
                     VSGATQIRAGVIRPEI"
     assembly_gap    6198..6207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6208..6441
                     /locus_tag="PK224_14110"
     CDS             <6208..6441
                     /locus_tag="PK224_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16926.1"
                     /translation="RTGAARPDRVEREGIQLGDPVRVIRDPLFGKIGAVSGLPSELRA
                     IPTESEVRVLEVRFPDGTTTVVPRANIEVIEGA"
     gene            6438..7685
                     /locus_tag="PK224_14115"
     CDS             6438..7685
                     /locus_tag="PK224_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16927.1"
                     /translation="MSRIQPRLRILSLAIAMLACLPSLLLAQTLPQQLRVIDTPSDGG
                     GSLTVLWAPSASDSAAWKYQILLHEGSVPSDPSAWKVVAEFPANTRYVREGKTAWWTR
                     SGEPGDHIFLLKNGKGIELKANQSYAVTVAAANGQERVIALPIVASPEPNWMNWNQLN
                     NLVLALMFGGVVFYAINLAKRKEIFLRRIPGLDAVDEAIGRATELGKPILYMTGAHDM
                     NDPSTIAAAVILGRVAKKAAAYETELLVPHREPITMAVCQEITKQAYMEAGKPDLFKD
                     DANFFITSDQFSYTAAVDGIMLRRKPAANFFMGSYFAESLLLTETGASTGAIQIAGTD
                     SDHQLPFFVTTCDYTLIGEELYAASAYLSKEPIQIGTLRGQDLGKAFVLSVIGIGTLL
                     ATIAVVTGNNWAQPLLDLFKDLK"
     gene            7682..8332
                     /locus_tag="PK224_14120"
     CDS             7682..8332
                     /locus_tag="PK224_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16928.1"
                     /translation="MMFLRRQLPLLITMITGLVMAAQYYVPHPASEQLLTTVTKWLQI
                     IGGFALVLGITSLFHVHASKIRRKEAGWAYSLVLYVGMLGTIIVGLWNGGKESIDGAL
                     TSFGWVYSYTLVPLQGTMFAILAFFIASAAYRSFRARSREAAVLLVAAVIVMMGRVPL
                     GEYMLPISGDLSSWILNVLNASVRRAILIGVSLGAVALSFKIIFGVERSYLGGGKE"
     gene            8485..9330
                     /locus_tag="PK224_14125"
     CDS             8485..9330
                     /locus_tag="PK224_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16929.1"
                     /translation="MTFAERMLRIDRRIIFLVIGLCTLLPLLFPVGLPIKISSEVRGV
                     YDYIEALPEGSVFLLSLDFDPASKPELYPQAIAILRHAFKKNLRVVGMTLWVSGTGLA
                     DQILSQTAKEAGKENGKDYVFLGWSPGGSAVILNMGQDLYNAFPADYGGKATKGLPVL
                     AGVQNLRDVTYAVSLGAGNPGVEAWYVFGKDKYKFELGGGCTGVIAPGLYPLLRSGQI
                     NGLIGGLRGAAEYETLIGQKGKAVAGMDAQSATHLAIIVLVAICNIFYFTLRRQQRRH
                     DGLGS"
     gene            9335..10090
                     /locus_tag="PK224_14130"
     CDS             9335..10090
                     /locus_tag="PK224_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16930.1"
                     /translation="MDLSFDVILGAWIATGLTLFILSFLYEDNPLFKLAEHLYVGVSL
                     GYTIVKTYDTVVMTLILRPILDKGEWSLLIPVAIGMLMLTRYVPKAAWLSRYAFAFIV
                     GIGAGLAIPRTISSFILKQIEDTVRPLLAVAPGGGITFDYSLLNPASHVNAIIILIGV
                     VSVLFYFFFSVEHSGPGKAVARAGILFLMISFGAAFGYTVMARMSLLIGRLTDLIEFS
                     DASYGRPTLWLVLLTVATLIVLSRRGSAHPPKQ"
     gene            10249..11511
                     /gene="amrB"
                     /locus_tag="PK224_14135"
     CDS             10249..11511
                     /gene="amrB"
                     /locus_tag="PK224_14135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRB16931.1"
                     /translation="MPLIMESTPTIAAKDPAQYPLLRNLQFSPIKEGEEQYIVLWDPT
                     GLSKERLVLPLNYFFIVQHLDGEHSVQDIGAIYLKRFGEFLMPNKVEQLLADLDTKLF
                     LEGTRTEQAKQQALTAYRQAPARLPQFSGRSYEADAAKLRAQINGFFTSKEGPEVKAS
                     EHAGKLIKGLVAPTYELKHAGPIYAWAYKELQEAQQPDLYVLIGTAYSGLEQPVAMTD
                     KDFETPLGLVKVERAVTDRLREQIPSAFTDELAHHNEHALEFQLPFLQESLGKDRPFT
                     IVPILTSFSADSLRDPQIREQVETFLQALKDVLVATGKNYCVVVGAELAHIGMRYGDS
                     APPTDFSFHRCMQTDLEMLKHVENRDPEEFAKFIQKENDQRRISGFSPIYSLLRLIQA
                     ETGQVLRYDRGITDQYNSTVTYASMAFF"
     gene            complement(12402..12701)
                     /locus_tag="PK224_14140"
     CDS             complement(12402..12701)
                     /locus_tag="PK224_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16932.1"
                     /translation="MTDHYPSYPDGVRWAIDRGKILRGMNQDQVYLALGSPVCKKDVE
                     DEGRMVTVWLYPPIGRDACITSAFRVYFEEGVVTTWDRFTTPTRYTDPAGGMPAY"
     gene            complement(12812..13342)
                     /locus_tag="PK224_14145"
     CDS             complement(12812..13342)
                     /locus_tag="PK224_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16933.1"
                     /translation="MPTITAERLILLLEQFVKTTGQPIDLLLIGALALQCYGFRDRYT
                     NDVDGELTGPLHPLVAFLSAEGVPADLTQDISGWSIVAMPPGYRDRATDLVSHPNLRI
                     RLLEPVDFVIAKLRRGIELDLDDALYVVRRFQISRSDIEQAAAAAIAASPEDTALFLF
                     QKTVELFCRQLPEQML"
     gene            complement(13323..13658)
                     /locus_tag="PK224_14150"
     CDS             complement(13323..13658)
                     /locus_tag="PK224_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16934.1"
                     /translation="MDVPPQTPIPSFKLLSAYLNTDSVAEALGTARGRRILWLEILVN
                     DQPDWSQWRQDPEFEAVYLNACRWYAHYRRLITFLFNRTPLPDDSGPIDFRDYRTFAE
                     ALQFAYHHR"
     gene            complement(13803..14324)
                     /locus_tag="PK224_14155"
     CDS             complement(13803..14324)
                     /locus_tag="PK224_14155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRB16935.1"
                     /translation="MIMQFQKKDPRATIITRFGEIRIKFYPDDAPRHVENFINLAKMG
                     FYDDTTFHRVVPGFIIQGGDPLSKTPDRMLHGTGGPGYFLNPEPNDRPHKRGAISMAK
                     MPRDGNSTRDFNDNGSQFFISIQDNGGLDRRYSIFGEIIRGIEIIDMIAKLPCDERDN
                     PLEAIRMKVTVKE"
     gene            14742..16265
                     /locus_tag="PK224_14160"
     CDS             14742..16265
                     /locus_tag="PK224_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB16936.1"
                     /translation="MPDTMVLNVDLADVWEQRGRKKLIRTIAWGDEVTVLKVAATHLE
                     VAITVFREKPDGSILPESITGYIEPPKPIKIANLTRPLADNQVLKVNFVDVQQGDGSV
                     IESPDGKIILVDGGDNQMFARYLAGRFRGTTAEKPQPIDCILVTHGDADHFAGLPEIL
                     KSETNKVKRKRLFMQPKRVYHNGIVKRPSKINNKTVPDTKLLGPTKTVNGQLYLTGLE
                     DNLLDVADTEMNEPFREWKNTLATYNSRSEVAFRRLQFGDNQAFEFFNRGDLRIETLG
                     PMTTTVSGQPALKFLGEPPKGPRIGHDSLSDSDEGFTGHSASHTINGHSIVFRLVYGG
                     FSFLFSGDLNDEASRTLAREHNRGNLNLRSEVFKVPHHGSADFSGAFIQAVSPIVSVV
                     SSGDESARKEYIHPRATLMGALGKWSRVPEPLIFVTELVAFFEEEGLSRLQDEKKAKK
                     RGPFYGFSRTAYGLVKTRTDGTRLFVYTDSGNVQMKEAYAYALDSSGVPQPAEVKRA"
     gene            16423..16749
                     /locus_tag="PK224_14165"
     CDS             16423..16749
                     /locus_tag="PK224_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16937.1"
                     /translation="MRSSRLLAALLLSASASAATAWAEPGFDEKYQRDFNIFNPINQY
                     QPGNPLNPANAYDPANPFNPINQYDPNNPANPINQYRPENPLNPINKYNPNTPFAPLG
                     GPGQKR"
     gene            16967..19030
                     /locus_tag="PK224_14170"
     CDS             16967..19030
                     /locus_tag="PK224_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRB16938.1"
                     /translation="MQWIAPSEKDTATDALEKRLWDAADQLRANSGLNAAQYSTPVLG
                     LIFLRFTDVRFAKIRAGLEKMASSSRRGSRVDDPAAYHAEGVLYLTPNARFDKLLHLP
                     EGSNAGKTINEAMRDIEKHNPQLAGVLPKTYEIFNSTLLKELLKRVSEIPATIDYDAF
                     GRIYEYFLGEFARTEGQKGGEFYTPSGIVRLLVEVLEPYHGRILDPACGSGGMFVQSA
                     RFVAEHKKNPSSELAIHGQEKVAATVALCRMNLAMHGLEGDIKEAITYYDDLHNSTGK
                     FDFVLANPPFNVNAVDKERLEAEIGKGRRYPFGLPRTDNANYLWIQLFYSTLSGNGRA
                     GFVMANSASDARSSEQEIRKQLIEARAVDVMVAVGPNMFYTVTLPCTLWFFDKAKAKL
                     PCKTPSPGSANHPLPKAEGRGGFKFQGLVDQARELRKKQTPAEDVLWQLLRDRQFADL
                     KFRRQHQIGDYIVDFFCPKYALVVELDGAVHETSDRKKKDTKRDTYLRSLGHTVLRFT
                     NAGVFDDTEGVLREIVSSRPSPSGRGEGEGEQRGARYSDTVLFIDARHIYRQIDRAHR
                     DWSVGQIGFLANVVRLYRGEEPDFTLGGPEAEAKLKEVFGKKLKFADVPGLCKGATIK
                     EIEAQGWSLNPGRYVGVAPGEDVSDEDFKEQLETLNEELEALNAQARELEITIARNVA
                     EILEA"
     gene            19048..20232
                     /locus_tag="PK224_14175"
     CDS             19048..20232
                     /locus_tag="PK224_14175"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRB16939.1"
                     /translation="MIRTKLKDVCDRITVGHVGPMAEEYTEEGVPFLRSQNITPFSLH
                     LHDIKYVPPTFHEKLKKSALRPGDVAVVRTGYPGTACVIPESLPEANCADLVVITPSE
                     KLNPYYLAAIFNSAWGKSSVAGNLVGVAQQHFNVGAAREMEIALPSRAMQDQIAGILS
                     AYDDLIENNQRRIKILEEMARSLYREWFVHFRFPGHDKVKMVSSPLGPIPQGWEVKPL
                     EELATVTMGLSPKGDTYNEEENGTPLVNGPVEFGDRFTKRMKWTTEPTKFCKEGDLVV
                     CVRGSTTGKYVKSDGVYSLGRGVCGISSKYQCFIELLFENELPTLLGQTSGSTFPSWT
                     GPQLKSHRVLSPPPEVSARFDALVKPMSATVLRYSRQIQNLRRTRDLLLPRLLSGNLD
                     LN"
     gene            20236..22053
                     /locus_tag="PK224_14180"
     CDS             20236..22053
                     /locus_tag="PK224_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB16940.1"
                     /translation="MRLKEVGIKNFRGYRNETRMPIDPNVTGITGKNDAGKSSLLEAL
                     DIFFEGGEIALEKDDFNVNEPDGMVEIRCIFDNLPSEIVLDETNRTTLQTEHLLNSAG
                     DLEILKRYKRSALKDPSIFLIANHPTATAFDDLHTLKITDLKKRAREKGVADSEVADG
                     RASASWRLAIWGKAPDLQREPRELEISKFAGDSKTIQDKLFKQLPLFALFKSDRESKD
                     NDPQAKNPLQEAVKQAQAELKAQIETIQVEIQNRVLERAQKTLEKLREMDPSLASQLV
                     PRFKKAPSWTFEFALDGEDGIPINKRGSGVRRLILLNFFRAESERKVADKNAPSVIYA
                     FEEPETSQHPSNQEMLVRALVQVGAKDNCQALVTTHVPALAGLLPVSGLRLAEKTPSG
                     TTIEYGSDTVIDRIAQSLGVLIDPRASRAKGLLLVEGPSDVVFLRHTAEQLKAGGYIS
                     ATLEERGLLPVSIGGCDLLKHWIAKRIAEQFNIPWALLLDSDSGTPENGKNLAQVQAL
                     KQLGKKAYVTRKREPENYILPEVVLPHITCGVTVTWTDTSDAKKDIGSATSKSQGEVL
                     EFFWTQMNAEQIRRAEKYIEDGQEKFELTEILSDFLTLS"
     gene            22060..22263
                     /locus_tag="PK224_14185"
     CDS             22060..22263
                     /locus_tag="PK224_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16941.1"
                     /translation="MAKGFSEDFLVEQPAIQLFSEMGWQTLSALEEVFGVGRTLGRET
                     KGEAVLALRLRAALVRLLPLHFE"
     gene            22287..23216
                     /locus_tag="PK224_14190"
     CDS             22287..23216
                     /locus_tag="PK224_14190"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HRB16942.1"
                     /translation="MPPPESYPIIALRKDDVLAPEEMGSKRKGWVQVAGDSERWLFKY
                     ARLSNGQVTGEHWAEKVAAEVAGLLHIPHARVELATLDGSPGCISRRFPELARPGAEL
                     IHGNDLLAGAGFGYDRAKQFRQSDHTVENILAAISHVFSDPAEQATAFQQMAGYLVLD
                     AIILNTDRHHENWALIRVMHQDGRVTHEMAPTFDHASSLGRNEPPDKLVAWLTEPGRP
                     EWYAGRNRCQGGIYLRSDDAHGANPLKLLELVARKWPDYFAPWRPQVENVQEEALCDT
                     VGRVSAGTMVQAQREFAKALLRVTLRRVKDILL"
     gene            23213..23983
                     /locus_tag="PK224_14195"
     CDS             23213..23983
                     /locus_tag="PK224_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HRB16943.1"
                     /translation="MKTLFLAWQDKRLRGDHVDGTRAWFPIGRLDAEPDDAHYRFAYT
                     RGVLQAKEKAGFQPLDAFPKFDALYESSELFPLFQNRLISTKREDYAEYLQRLDLSPE
                     CADPFEILAISGGGRQTDNLEVFPKIQARRDGSFSCRFFLHGWRHVNAPSQDRLNGLR
                     PGEPLQVSIELNNPATGLAVQLETANDYFMLGWAPRYLIQDVLRAVAGGPSNMTARVV
                     QLNPPPAPHNQRVLVELEGRLTAGYEPMSSDEFLPLRS"
     gene            23990..27169
                     /locus_tag="PK224_14200"
     CDS             23990..27169
                     /locus_tag="PK224_14200"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRB16944.1"
                     /translation="MAHAYSEDQLVEQPAITLLAQLGWHTISAVDEVLGPHSTLGRET
                     KAESVLLLRLRAALCRLNPALPPEALSTAIDELARDRSAMSLVAANREVYGLLKDGIP
                     VSVPDHIGGGQKTERVRVIDWENPAANDFLLVSQFSVTGSLYTRRPDLVGFVNGLPLV
                     VIELKKPGVPAQQAFDDNLTCYKADIPQLFWFNGLMIASDGTESHVGSLTADWERFFE
                     WKRIEREDEPRRVSLEVMLRGTCEPSRLLDLLENFSLFSEHKAGLVKVLGQNHQFLGV
                     NNAIAATLAARKQGHGRGGVFWQTQGSGKSLAMVFFAQKILRKIPGNWTFVVVTDRVE
                     LDDQIAKTFKACGAVSETESELCHAQSGAQLRQLLGENHRYIFTLIHKFQTPELLCDR
                     SDVIVLTDEAHRSQYDQLALNMRAALPHALFLAFTGTPLIAGEERTREVFGDYVSIYD
                     FQQSVEDGATVPLFYENRTPELRLENPNLNDDIYNLIEAAGLDDDQEKRLERELGRQY
                     QLLTRDERLETIAQDIVQHFLGRGFQGKAMVISIDKATALRMHDKVRAQWKSEEKRVE
                     QELTRLTTYGDKAADPDRIRELRARLEVIRTTDMALIVSAAQNEISDMKKLGLDIAPH
                     RKRMNDEALDEKFKEAKDPLRLVFVCAMWLTGFDAPSCSTIYLDKPMRNHTLMQTIAR
                     ANRVFPGKHSGLIVDYANVFASLEKALAIYAKGRGGEQPIRDKQKLVESLRQAIAEAE
                     AFCLAQGVSLAEIEQTPAGSLDRLTKIAEAVECLISPDPLRKDFLGHEGWVRTLFRAV
                     KPDPSALECASRVACLTTIAETIRERTGEGPADISAVMADLNKILDASVAADGFHIPQ
                     GKSGHGVIDLTKIDFEALAKRFGKSKTRNIDLEQLKAAIRSQLNTLVRLNRTRADYLT
                     KFEELIESYNAGSRNIEELFKELLALSRSLNDEQERHVRERLSEEELVVFDILTRPAP
                     QLSVEERAEVKKVARDMLQKLKQLLVLNWRQKASARSQIKLTIEDLLDQGLPRAYTPD
                     LYQQKCSAVFEHVYETYGDRGASIYPAGR"
     gene            27778..28257
                     /locus_tag="PK224_14205"
     CDS             27778..28257
                     /locus_tag="PK224_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRB16945.1"
                     /translation="MSHHGKTKAQKGSLPAMTVTPVAAAIGVVLRDDQVLLVRRANQP
                     DAGKWGFPGGKIEPGEPVQAAAAREVAEETGLVVRPLHVFTAVDAFHRNASGQLVTHY
                     VLIAVLCEWISGLPQAAGDALEAEWFPIETLDSVDIVMSVDVARVASMAAKVRDDVQ"
     gene            complement(28590..28919)
                     /locus_tag="PK224_14210"
     CDS             complement(28590..28919)
                     /locus_tag="PK224_14210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRB16946.1"
                     /translation="MDMVVSRFDVFLVRLDPTQGREIRKTRPCLIISPDEMNQHIDTV
                     IIAPMTTKGRPYPTRVSLRFKGKSGQVVLDQIRTVDKSRLVKRVGTITDVTRAQILTL
                     LAELFAP"
     gene            complement(28907..29161)
                     /locus_tag="PK224_14215"
     CDS             complement(28907..29161)
                     /locus_tag="PK224_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRB16947.1"
                     /translation="MKARVVRIGNSQGIRIPKSVIEQCHLHGDVDLEIQHGQLVIRAA
                     SKPRAGWRQAFEQMHRHGDDHLLDEESAAMSTWDRSEWTW"
     gene            29425..29550
                     /locus_tag="PK224_14220"
     CDS             29425..29550
                     /locus_tag="PK224_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16948.1"
                     /translation="MAQEIERVLQPPTEVRLSRFDRAVRLFYERYAKTKDGGKWL"
     gene            29691..30419
                     /locus_tag="PK224_14225"
     CDS             29691..30419
                     /locus_tag="PK224_14225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HRB16949.1"
                     /translation="MPSMVPPFTATQAAVLKNFLASPQRPTGTLTYPQLAGFLFSLAN
                     GPELIPPSEWIPMVFDDHDAGYDTQAEAEQVLQAMMALYNDCARQYFADRPTLPPGCD
                     IRPDPLDNLEPDAPLSHWAQGFGMGHDYLVEYWDEFTPEELDEALGAVLMTLTFFSSA
                     SLARAYHEEGKAGTSLAQLAGTVLDIFHDALGEYAHLGRAIYQGRCEAGDLSPAPTTG
                     RKVGRNDPCPCGSGSKFKKCCGAT"
     gene            complement(30934..31170)
                     /locus_tag="PK224_14230"
     CDS             complement(30934..31170)
                     /locus_tag="PK224_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16950.1"
                     /translation="MSTITVTLPDELLNRCTRYAQALHISRAAYIRQSLERMNLETER
                     QLRVDRLTQASYKVRDQSMRINGEFDAIETDLYA"
     gene            complement(31291..32535)
                     /locus_tag="PK224_14235"
     CDS             complement(31291..32535)
                     /locus_tag="PK224_14235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB16951.1"
                     /translation="MGTGRATTQPRWGILGLLFAISAVTYMDRVNISVTARQMMPAYG
                     LTDQDMGYIFSAFVFGYALCQIPGGWLGDRWGARVVLAGALVWWSLFTALTAVAATLP
                     LAAMVGTVGALIVVRFLLGVGEAVALPNFNRAVADWIPPAQRGLGIGIAIGGIGIGAA
                     ITPPLASWVMVNYHWQTVFYLSALIGLVVALLWVFCSREQKAAEGVASVHPRVVVPWR
                     QFAASSSLRWLVLSYACLGYVAYIYMSWFYLYLVNVRGIDLLRGGWLAAAPFVAILVF
                     CPLGGWTTDRLVSTLGLTKARMIVGMIGMGLAGGLIAVGAWADSQAVAIASLSLGAGW
                     LYFTVGAYWSVTTDLSKTHAGTLSGVMNMGANVGGVISPSLTPWLADHWGWTASLLVA
                     ALIALCGGVMWMKIDASEGLRE"
BASE COUNT         7165 a         9773 c         9478 g         6395 t
ORIGIN      
        1 caatatcatt cccggcgtga aacatgtggt cgccatcagc agcggcaagg gcggcgtcgg
       61 taaatccacc gtgtcggtca atctggccgt cgccctcgcc ctcaccggag ccaaggtcgg
      121 attgctggat gccgatatct acggccccaa catcccgatg atgatgggcg tcgaaaaaac
      181 acctgaacag aaagacggca agatcgcccc ggcggagagc cacggcgtca aactgatctc
      241 catgggattt tttgtgccgg aagataccgc cgtcgtgtgg cgtggtccga tggtccacac
      301 cgccattcaa cagctcttcc gggatgtcct ctggggcgcc gtggcttttc tgttgctggg
      361 ccgggcgccg ggggccgggg gcgggggggg gcgccggggc tggccggggc ccccgggggc
      421 cggccgggct ggggcgactt ggattatctg ttgatcgacc tgccgccggg aaccggcgac
      481 gcgcaactga cgctgaccca gctggtgtcc ctgtcgggcg ccgtcacggt gacgaccccg
      541 caggaagtgg cgttgcacga tgtgcgcaaa ggcatgatga tgttccagaa ggtcaacgtg
      601 cccctgctcg gcatcgtcga gaacatgagc tttttcctct gcggccattg cggcgaacgc
      661 accgagattt tctcccatgg cggcggagaa cgggccgcgg agaaactggg cattccgttt
      721 ctcggacggg tgcccatcga tccggccatt cgcgccggcg gcgataccgg gaacccgatc
      781 gtggtcgcca agccggattc accgcaagct caggcgtttc gcgagatcgc cgcgaaactt
      841 gcggcagcat tacaaacggg cgaggcaggg gcggcaaaga gccggatcgc cagtctgtta
      901 gacaaaatca aacgaccggc gacgggaaat taaccggacg cccgatcaag gaagctggac
      961 gcactcaggg aggatgtatg ggcgagctga tcagaatcgc aggaaccgcc gacgtgaagc
     1021 cgggagccgg catggtggcg gaagtcaacg gtaaggccat cgcggtgttc aatgtggatg
     1081 gcgcctttta tgctatcgac aatacctgtg tccaccgggg cggcccgctg ggcgaagggg
     1141 atgtggaagg ggatgtggtg tcctgcccct ggcacaactg gcagttcaac gtgaaaaccg
     1201 gcacctgcgt caacaacccc tcggcgaaag tgaccgcgta cgaagtcacg gtcgaaggca
     1261 ccgacattaa agtccggctg tgacgcaaga ccggcccgcg ccggtcacca ggtcacggca
     1321 gcgaatgaat gtttccggca ccacagtgaa tggaggctcc gatggcagac aatacgctga
     1381 ccccgaaaca cattgagatc gccgaaacct tcgtgcggct ctacgtcttt ctgacgcagt
     1441 acatcgaccg ttgcgaagat gaagccgcgc gcaaggaata cccggaagca gaactccaaa
     1501 agcacctcgg cgaaacccgc gccaagatga tggagatcct gaaggtgaac cccgtcgtca
     1561 agggcaaggt ggaaaaagag tgtgaacgtg tcctcgcatt gggcagcaag tgtctcatgg
     1621 gtggaaccga aaaggtggcc gcgctggatg tgctgggagc cgagcgggtc attctcaaga
     1681 acaagaccat cgccttgagc gatctgttag ccgtgtatcg cgcgctctaa tcgttcgatg
     1741 tccaccccgg ctggagagaa acaccaactc gccggtttcg acgccaggga acgagggttc
     1801 aatcgtcccg tggaattcgt gcggacagcc tcggggtacc gggcgacgtt gcggtatgaa
     1861 acgacgcaca tcgtcacccc ggaagcgccg agcgcaggcg aggccctgca acaattgatc
     1921 acgaccctcc agcaagaggg ctacacccag ctgcgcagcc agcagagcta ccgcgacggc
     1981 ttgtacctcg gatcgcagga gctgtgggtg gaatatccgg accggcaggc agccccgcag
     2041 gtcggactcc tgggcctgct gaagaagtgg ctcggccgct caccgggctg agggtcgcca
     2101 caaacccctc gccgcaccgc ggcgagattg actctcctcc gcctcacgcc tatagtccat
     2161 ccaggtcagc gactgatcgt tcgtccgtca gccttcaccg gacaggatcc ggcaccggag
     2221 aattgacgat gcgctgggtt cacgtcctgc ttctcaccat catcgtgctg ctcccggcgg
     2281 gcaaggcgac cgcgaaggtc taccgttgcc aaggcgccag tggcagcaca gtcctgacag
     2341 accagccgaa gggcaagacg ggttgcgtcg ccatcgacac cgccaccccc ccgctcccgg
     2401 gaggattcac cccgccggtc gagccgagcg taccggcaca ggtggaactg ccggcgaatg
     2461 cgatcccgcc gtccatttcg gccgcaccga tggttcctca ccaacccggt cccggtgatc
     2521 ctgcgttatc gccggccgtt gcgaaggatg cccccgagaa ggccccacca gaagcgcaac
     2581 gttgctcacc acgcgtcaac ccgctgaatc ccttcgccgg aatgaattgc tcaccggcat
     2641 ccggcgaacc cccggcagag aacaagaacc agtagcccct cgtcgcgcgc gtcgccacag
     2701 gctcgattga ctctcccgat gccccttcat ataatgccaa aacgaaccgt ttaagctgat
     2761 ctcccaagca cttatgtctg ccttctccgc tggtttttta tcccgtcgcg cacaggattt
     2821 tcgagccggt ctccggcacg catttgccgt gcgtcctgaa caagcagcct tcacgatcga
     2881 agacctggcc ctgctcgaac gggtcgccga cgccgtcgtt tcgcgcggca tggccgcacc
     2941 ggccacattg tttctcgact cgatgggccc catgaacttt ctcggcagcc aggcactgca
     3001 ctttctggct cccgtcgtgg aatgcgtgct gaccggtacg gagatcgcgc aggtcgcgcg
     3061 cctgctggaa cggcgcgatt ccattcaccg cctgaccgcc ctcatcgaag ccaaggccgc
     3121 ggctccccaa ggagcgcccg ctcgatgacg gcacagcctc actcgccggc ggcaccagca
     3181 gtgactcatc ccttgaacgt catcgtcgca accgattgcg gcagcaccac caccaaagcc
     3241 attttgatcg aaaaggtcgg ggaggagtat cgccagacct accgtggtga agcaccgacg
     3301 accgtcgaag ccccgttcga agacgtgacc cgcggcgtgt taaacgccat cgcggaaatc
     3361 gaagaactct ccgggcgcac gattctggac ggcgaccgca tcatcacacc caaccaggcg
     3421 gctcagggcg atcccaagcg aggcgtggac atttacgtgt ccaccagcag cgccggcggt
     3481 ggattgcaga tgatggtcac cggcgtggtg cagaacatga ccggtgagag cgctcaacgc
     3541 gccgctctgg gcgccggcgc catcgtcatc gacgtcttgg cctcgaacga cggacggctg
     3601 ccctacgaaa agatcgagcg gattcgaacc atgcgcccgg acatgatcct gatgtccggc
     3661 ggcaccgacg gcggcgccgt gacccatgtg gtggaaatgg cggagtatat cgccgccgcc
     3721 gatccgcgtc cgcgcttcgg cagcacctac cggctgccgc tcgtgtacgc aggcaacaca
     3781 gacgcccggc cgcaaatcaa aaccatttta ggcgaaaaga ccgccttgga ttttgccgac
     3841 aatatccgtc cggtccttga gcgcgaaaac cttgcgccgg cccgcaacaa aatccacgac
     3901 ctgtttctcg aacatgtcat gcagcaggct cccggctaca agaaactcat cgagatggcg
     3961 ggcgcgccca tcatgccgac cccggcggcg gtcggcgtca tcatggagac catcgccaaa
     4021 cgcgaaggca tcaacctgat cggcgtcgat atcggcggcg ccaccaccga cgtcttctcg
     4081 gtgttcaacg gactcttcaa ccgcacggtc agcgccaacc tcggcatgtc ctacagcatt
     4141 tcgaatgtac tggcagaagc ggggctcgac aacatcatgc gctgggtgcc gtttaccatc
     4201 gacgaacaga ccctgcgcaa ccgcatcaag aacaagatgg tgcggcccac gacgattccg
     4261 caatcgctcg acgaannnnn nnnnnctgca gatcgaacat gccatcgcgc gcgaggccct
     4321 gcggctggcg ctgatccacc acaaatcgct tgcgacggga ctcaagggca ttcagcagga
     4381 gcgcaccatt tccgatgtgt tcgagcagcg gacttccggc aaaaccctga tcgatctgtt
     4441 gaaactggac ttgatcgtcg gcagcggcgg catcctgtcc catgcgccgc gccgtattca
     4501 gtccatgctg atgatggtcg atgcctatga accgctcggt gtcacgacac tctcggtcga
     4561 cagcatcttc atgatgccgc atctcggcgt cctctccacc gtgaacgagc aggccgccac
     4621 cgatgtcttt gtccgtgatt gcatggtcta tctcggcacc tgcatcgccc cgatcggaca
     4681 ggggaaagac ggggagcgct gcgccgacta cgaaatcgcg ttacccgatg gacgaatcga
     4741 gaggggccag ctggcgttcg gagatctcaa actcttcgcc ttggcgcggg agcagcaggc
     4801 cacggtgacg atgcaaccgg ccaaacagat cgatctcggg aacggaccgg gacagtcgtt
     4861 cacaaaaacg gtgaagggcg gcgtggtcgg gctcatgctc gatggacgcg gtcgtccgtt
     4921 gcagttgccg ggcgagcagg cagcccgcgt cgccatgctg accagatggt accaggcagt
     4981 agggctatac ccggtgggga gctgagagtc gaatgctggg agcggatggc gtatagctta
     5041 tggtgaagag cggggagcat ataacagatg gcaaggagaa gagaagcctc gcaccgcatg
     5101 ctggatgcaa actgttcaca gaaagcattt gcagttcgct cttgtatgaa gactagctcc
     5161 tatcggctat tagccatcag ctcttatctt gaggaggttg ctcaaaacgc cctgcagcga
     5221 ggccgcaagg cgcgagagcc ccgagtcgta ccggaagtcg tacagtgagg ggagcgagca
     5281 actgagaacg aagcggtagg gcgttttcag caaccgacaa tggcgcacag ttacacacca
     5341 ggcctgaccg tcaccgaaca gaccaccgtt cgccgcaggc gtattctgcc gctgccgggg
     5401 accgtcctgg tcaaggtggg tgacgtcgtg cgcgccgata ctgcggtcgc ccgtgccgag
     5461 ttgccgggca aggtcgttcc gctcaatttg gccaaccagc tgggtattgc gccggatgag
     5521 atcaacgact atttgatcaa gaaggaaaaa gactcggtcc agaaagacga cgtgctggcc
     5581 gagaacaaac ccttcatcaa atggtttaag accgaaattc gctcgcccat caccggcaag
     5641 gtggagtccg tctccaccat caccggacag attctgttgc gggaaccgcc gcgcatcctc
     5701 gaattgcgcg gctatatcga cggctcgatc gtcgaagtgc atcccgaaca gggggtgacg
     5761 gtcgaaacct cctgcagtct ggtgcagggc atcttcggca tcggcgggga gaccagcggt
     5821 gaattggtca tgggtgtcac ggcgccggat caaccgctga cgcctgagct gttgacgccg
     5881 gccatgaagg gcaagatcgt gatcggaggc gcattcctct ccgccgccac catgactcgc
     5941 gcgaaggaac tcggcgtcat cggtctcgtc gtgggcggga ttcacgacaa agatctgcgc
     6001 gcgctcctcg gatatgatct gggcgtagca attaccggca ccgagcaggt gggcttcacc
     6061 ctgatcctga cggaaggctt cggcacgatc ccgatggcgc ccaagacgtt cgcgttgctg
     6121 tccgcccacg cagggcacaa agcctctgtt tcgggtgcga cgcaaattcg cgccggcgtg
     6181 attcgtccgg aaatcatnnn nnnnnnncgc accggagccg cccggccgga ccgggtcgaa
     6241 cgcgaaggga ttcaactcgg cgatcccgtg cgagtgatcc gcgatccgtt gttcggcaag
     6301 atcggggcgg tgtccgggtt gccctcggag ctcagagcga tcccgacgga gagtgaagtc
     6361 cgcgtgctcg aagtccggtt tcccgacggc acgaccaccg tggtgcctcg cgccaatatc
     6421 gaagtgatag agggagcatg agccgcatac agcctcgcct tcgcattctg tccctcgcca
     6481 tcgcgatgct cgcgtgcctg ccgtcgctcc tcttggcgca gaccctcccg cagcaacttc
     6541 gagtcatcga cacacccagc gatgggggag gcagcctcac ggtcctctgg gctccttcgg
     6601 caagcgacag cgccgcgtgg aagtatcaaa tcctgttgca tgaaggcagc gtgccttcag
     6661 acccgtccgc atggaaagtc gtcgcggaat ttccggccaa cacccggtat gttcgcgagg
     6721 gtaagaccgc ctggtggacg agatcaggcg aacctggcga ccatatcttc ctgctcaaga
     6781 acgggaaagg catcgagctc aaagcgaacc agtcctatgc cgtcacggtg gccgccgcga
     6841 atggacagga acgggtgatc gcactgccga tcgtcgcctc gcctgaaccg aactggatga
     6901 actggaacca actgaacaac ctggtgctgg ccttgatgtt cggcggggtc gtgttttacg
     6961 ccatcaacct ggccaagcgg aaagagatct tcttgcggcg tattcccggg ctggatgccg
     7021 tcgatgaagc catcgggcgc gccacggaat tgggcaaacc gatcctctac atgaccgggg
     7081 cgcacgatat gaacgaccct tcgaccattg ccgccgccgt catcctgggg cgcgtggcca
     7141 agaaggccgc cgcgtatgag accgagttgc tggtcccgca ccgcgagccg atcaccatgg
     7201 ccgtctgcca ggaaattacc aaacaagcgt atatggaagc cggaaaaccg gacctcttca
     7261 aggacgacgc gaactttttc attacgagcg accagttcag ttacacggcg gcggtggacg
     7321 gcatcatgct gcgccgaaaa ccggcggcca actttttcat gggttcatac tttgccgaat
     7381 cactgctcct gacggaaacc ggcgccagca ccggagcgat tcaaatcgcc ggcaccgact
     7441 ccgatcacca gctgccgttc ttcgtcacga cctgcgatta caccctgatc ggcgaggagc
     7501 tctatgccgc cagcgcctac ctgtcgaaag agccgatcca aatcggcaca ctgcgcggcc
     7561 aggatctggg caaggcgttc gtgttaagcg tcatcgggat cggaacgctg ctggccacca
     7621 tcgccgtcgt cacgggcaac aactgggcgc aaccgctgct ggacctcttc aaggatctga
     7681 aatgatgttc cttcgccgac agctgccgct gctcatcacc atgatcaccg gtctcgtcat
     7741 ggccgcgcaa tactacgtgc cgcatccggc ctcggagcag ttgctgacca cggtcaccaa
     7801 gtggctgcaa atcatcggcg gcttcgcgct cgtgctcggt attaccagcc tctttcacgt
     7861 gcatgcgtcc aaaatccggc ggaaggaagc cggatgggcc tacagcctcg tcctctacgt
     7921 cgggatgctg ggcaccatca tcgtggggct ctggaacggc ggcaaagaaa gtatcgacgg
     7981 ggcgctgacg tcgtttggat gggtctactc ctacacgctg gtgccgctgc agggcaccat
     8041 gttcgcgatc ctggccttct tcatcgcctc cgcggcctac cggtcgttcc gggcacgcag
     8101 ccgtgaagcg gcggtgttgt tggtagcggc cgtcatcgtc atgatggggc gggtgccgct
     8161 gggcgaatat atgttgccga tcagcggaga cctctccagc tggattctga atgtcttgaa
     8221 cgcgtctgtg cggcgcgcca ttttgatcgg cgtcagcctc ggtgccgtgg ccctctcctt
     8281 caagatcatc ttcggcgtcg aacgctcgta tctgggcgga ggcaaagaat aacgcgtgag
     8341 acgtgaaagg ttagacgtga aacgtaagtg ccctcgtctc gactctctac cgtctgacgt
     8401 tttacgttcg acgtttcacg gggcacggag tgcccgcttt ccctttcacg tttcaccttt
     8461 tacgtttcag gtccgggagc aagcgtgacc ttcgctgaac gcatgttgcg gatcgaccgg
     8521 cggatcatct ttctggtgat cggtctctgc acgctcctgc cgctgctctt tcccgtgggt
     8581 ctgcccatca agatctcctc ggaagtgcgc ggtgtctacg attacatcga ggccttaccc
     8641 gaggggtcgg tcttcctgtt gtcgctggac ttcgatcccg catccaaacc cgaactctat
     8701 ccgcaggcca tcgcgatttt gcgccatgcc ttcaagaaaa atttgcgcgt agtcggcatg
     8761 accctctggg tgtccggcac cggattagcc gatcaaatcc tgtcgcaaac cgcgaaggaa
     8821 gcgggcaagg aaaacgggaa ggactatgtc ttcctgggat ggagtcccgg cggcagcgcc
     8881 gtcatcctga acatggggca ggacctctac aacgccttcc cggcggacta tggcggcaag
     8941 gccaccaagg gtcttccggt gctggccgga gtgcagaacc tgcgcgatgt gacctatgcc
     9001 gtgagtttgg gcgcaggtaa tccaggggtc gaagcctggt acgtcttcgg taaggacaag
     9061 tacaagttcg aactcggcgg cggctgcacc ggcgtcatcg cgcccggcct ctatccgctg
     9121 ctcaggagcg gccagatcaa cggcttgatc ggcggtttgc gcggagcggc ggaatacgaa
     9181 accctgatcg ggcagaaggg gaaagcggtc gccggcatgg atgcgcaatc ggccacgcac
     9241 ctcgccatta tcgtgctcgt cgccatctgt aacatcttct actttacgtt gcggcggcag
     9301 cagcgccggc acgacgggtt aggatcgtag ccgcatggat ctttccttcg acgtcatact
     9361 cggcgcctgg atcgccacag ggttgaccct cttcatcctg tcgttcctgt acgaggacaa
     9421 tccgctgttc aaactcgccg aacatctcta cgtcggcgtg tcgctcggct atacgatcgt
     9481 caagacttac gacacggtgg tcatgaccct catcctgcgc ccgatcctcg acaagggcga
     9541 atggtccctg ctgatccccg tcgccatcgg catgctcatg ctgacccggt atgtgcccaa
     9601 agccgcctgg ctctcgcgct atgccttcgc ctttatcgtg ggcatcggcg ccggtctcgc
     9661 catcccccgc accatttcgt cgtttattct gaagcagatc gaggacacgg ttcgcccgct
     9721 cctggctgtg gccccgggcg gcggcatcac gttcgactat tcgctattaa atcccgcgag
     9781 ccatgtgaac gccatcatca ttttgatcgg agtggtgtcc gtcctgttct atttcttctt
     9841 ctcggtagaa cattcaggac cgggcaaggc tgtcgcgcgc gccggcatcc tctttctgat
     9901 gatctccttc ggcgcagcct tcgggtatac cgtcatggcc cgcatgtcgc tcctgatcgg
     9961 ccgcctgacg gacctgatcg aattctccga cgcctcctac ggacgcccca cactctggct
    10021 cgtcctcttg accgtcgcca ccctcatcgt gctgagccgg cgcgggagcg cccatccgcc
    10081 gaaacagtag gcaccaggct ggcacccggt gccgtcgttc gtcgctcgta tctcgtcgtt
    10141 cgcaattcgt gccaagacac gcatcttctt tccccacgag agacgtttca cgtttcacgt
    10201 ttgacctttc acgactgacc ttgcgcctga gaagttcgct ccgctacaat gccgctcatt
    10261 atggaatcaa cgccgaccat cgccgccaaa gaccccgcgc aatacccgct gttgcgtaac
    10321 ctacaattct cgcccatcaa ggaaggggag gagcagtaca tcgtcctctg ggacccgacc
    10381 gggttgagca aggaacgact ggtcctgccg ttgaattatt tcttcatcgt ccagcatctc
    10441 gacggggagc acagcgtcca ggacatcggc gcgatctacc tgaagcggtt cggcgagttc
    10501 ctgatgccga ataaggtcga gcagctactg gccgatctgg atacgaagtt attcctggaa
    10561 gggacgcgca ccgaacaggc caagcaacag gcgttgaccg cctatcgcca agcgcccgcc
    10621 cgattgccgc agttttcagg ccgcagttac gaagccgacg ccgccaaact gcgcgcccag
    10681 atcaacggct tcttcacctc caaggaagga ccggaggtca aggcatccga acatgcgggc
    10741 aagctcatca aggggctcgt cgcaccgacc tacgaactga aacacgccgg accgatctat
    10801 gcctgggcct ataaggaatt gcaggaagcc cagcaaccgg atctgtacgt gctcatcggc
    10861 accgcctact cggggctcga acaacccgtg gcgatgacgg acaaggattt cgagacacca
    10921 ttggggctcg tgaaggtcga acgcgcggtc accgatcgcc tacgcgagca gattccttca
    10981 gcctttaccg acgaattggc acatcacaat gaacatgcac tggaatttca gctgccgttc
    11041 ctgcaagaga gtctcggcaa ggaccggccg tttacgattg ttccgatcct cacgtcgttt
    11101 tcggcggaca gcctgcgcga tccgcaaata cgggagcagg tcgagacgtt tcttcaggcc
    11161 ctgaaagacg tcctcgtggc caccggtaag aactactgtg tggttgtggg cgcggaactg
    11221 gcccacatcg gcatgcgata cggcgactcg gcgccgccga cggatttctc cttccaccgc
    11281 tgcatgcaga ccgacctcga aatgctcaaa cacgtcgaga accgcgaccc cgaagagttc
    11341 gcgaagttca tccagaaaga gaacgaccag cgccgcattt ccggcttctc gccgatctac
    11401 tcgctgctgc gtttgattca agcggaaacg gggcaggtgt tacggtacga tcgcgggatc
    11461 acggatcagt ataattcgac ggtcacgtat gccagcatgg ccttcttcta aagcaggctg
    11521 ctgaaaaagc cggccacctg cgttctcagc tcgacaaaat cctcaacgta gccagaggct
    11581 acgcctccgg ttttctctcg cctgcggcct tgttggacgg actttttgag cagcctgcga
    11641 agaaaattac gccgtcgttc tcgcgtctct cagatcgctc aacgtagcgc aaggctacgc
    11701 ctcgctcctt cgctagctgc ggcccaggtg gacaatcgtt ttgaccgttc tgcgggctgg
    11761 ctgctgaaaa agcccgccac ctgcgttctc agctcgacaa aatcctcaac gtagccagag
    11821 gctacgcctc cggttttctc tcgtctgcgg ccttgctggg cggacttttt gagcagcctg
    11881 caccggaagt caccccgtca ttctcgaatc gcctcgctcc atgaatggag cccgaagcta
    11941 cgcttcagtc ttcctgcctc gacgagtcac gttttacgtc tgatgtttga cgatactcgg
    12001 ctgaaggctg ttcagaaagg ccgttcagca aagccgcagg aagcacggcg actgaggagg
    12061 tacataccaa acttcgtttg acccgttcgc cgtgacagat actcctggcg aacggatata
    12121 ctccaccagt agtcccgttt tatctcccat acgtcgcagg gagacgagcg accgagaacg
    12181 ccgctgggcg gtctttctga gcagcctgct agcccgcatg attcatgacg gttcgtcgcg
    12241 aaattgcgga gtcgcaaagc gagacggacg cgcggagccg tgagggagga aggcatcctt
    12301 gaacaggatg tcgaccggtc gagcggcgag cccgcccgcc gacaggctag tcgcagctgc
    12361 gagtccgcga ttgcagcaga agcgctcatg aatcatgcgg gctagtaggc cggcatgccg
    12421 ccggccggat cggtatatct ggtgggggta gtgaagcgat cccaggtggt gacgacgcct
    12481 tcttcgaagt acacacgaaa ggcgcttgtg atgcaggcat cccggccgat gggcgggtag
    12541 agccagacgg tgaccatccg gccttcgtcc tccacgtctt tcttacacac cggcgacccg
    12601 agcgcgagat agacctgatc ctggttcatc ccgcgcagga ttttcccgcg atcgatggcc
    12661 cagcgcacgc cgtcgggata agacggatag tggtcggtca tcagtcgatg acgttccgtg
    12721 tcgcatccgg ataagcttat ggcaagcaga agaagactat ggagaaggac ccgtttaagc
    12781 gtcgccaacg aagcgcctct taggagagac ctcatagcat ctgttccggc aattgccggc
    12841 agaacagctc aaccgttttt tgaaacagaa agagcgccgt gtcttctgga gaggccgcaa
    12901 tagcggcggc ggcagcctgt tcaatgtcgg aacgggaaat ctgaaaacgc cggacaacgt
    12961 aaagagcgtc gtccaaatca agctcaatac cgcgacggag tttcgcgatc acgaaatcta
    13021 ccggttccaa aagacggatg cgaagattcg gatgagagac taggtctgtg gcacggtctc
    13081 gatacccagg cggcattgcc acgatagacc atccagaaat gtcctgcgta agatccgccg
    13141 gcacaccttc agcactcaga aacgcgacaa gcggatggag cggaccagtg agttcgccgt
    13201 ccacatcgtt cgtgtagcgg tcacgaaatc cataacattg caacgcaagg gcgccgatga
    13261 ggagcaagtc gatcggttga ccggtggtct tgacgaactg ctcaagtaaa agaatgagac
    13321 gttcagcggt gatggtaggc aaattgtagc gcctcggcaa aagtccgata gtcccgaaaa
    13381 tcgatcgggc ccgaatcatc gggaagcggc gtacggttga acaagaaggt aatcaggcga
    13441 cgatagtgcg cataccagcg gcacgcattc agatagaccg cctcaaactc agggtcttgt
    13501 cgccattggg accaatcggg ctggtcattc acgaggattt cgagccagag aatgcgacgc
    13561 ccacgagcgg tgccgagggc ctctgcgaca gagtcagtat tcagataggc agaaagcagc
    13621 ttaaaggaag gtatcggtgt ctgcggggga acatccatgt cgcgaagtct agcatagaga
    13681 acaacgagtg tcaggaatta taaacgcttc tggctcatcc cttagagctg atggcaggcc
    13741 acacaattct ctgactcccg ttccagcgat cggctataag ccattggcca tcctcgctgc
    13801 cgctactcct tcaccgtcac cttcatgcga atcgcttcga gcgggttgtc gcgctcgtcg
    13861 cagggcagct tcgcaatcat gtcgatgatt tcgatccccc ggatgatttc cccgaagatg
    13921 gaatagcgac gatccaatcc gccgttatcc tggatcgaga taaagaactg cgacccgtta
    13981 tcgttgaagt cgcgggtgct gttgccgtcg cgcggcattt tcgccatgga tatggccccg
    14041 cgtttgtgcg gccggtcgtt cggctcggga ttcaagaagt agcctggtcc gccggtgccg
    14101 tgcagcatcc ggtcgggtgt cttgctcagc ggatcgccgc cctggatgat gaaccccggc
    14161 accacgcgat gaaacgtggt gtcgtcgtag aagcccatct tcgccaggtt gatgaaattt
    14221 tcgacatggc gtggcgcatc gtccgggtag aacttgatcc tgatttctcc gaacctggtg
    14281 atgatcgtgg cacgcggatc ttttttctga aattgcataa tcatgggacc aaatctaaca
    14341 gggcggggat tccaacggca agagggcgga tccggcatgc atcggacggc gagtcacgaa
    14401 cgacggcctc cgttttgaca ggcgtgattt ctcacggtga acggcgtatt ctctgccgaa
    14461 accatcgagc cgccgatgac cgggcttagc ggacggttac gcctaatgga aacacaggcg
    14521 ggaactcacc tgcacccgca ggctgctcag caaagccgtc cagcaaggct gcagcgtgag
    14581 accagggaca ctacaattgt tcgcaggctg ttcagaacgg ctgtccggca aggccgtagg
    14641 aagcacggcg actgaggcgt acccttgcgg tacgtcgcag ggagacgtgc gaccgggaac
    14701 gccgccggca gtcttactca accgcgtgcg agggagggcc tatgcctgac acaatggtgc
    14761 tgaatgtcga tctggcggat gtgtgggaac agcggggacg gaagaagctc atccgaacca
    14821 ttgcctgggg cgacgaagtc acagtgttaa aggtcgccgc cacacacctg gaagtggcca
    14881 tcaccgtctt tcgcgagaaa cccgacggca gcatcctgcc cgaatccatc accggctata
    14941 tcgaaccacc caaacccatc aaaatcgcca acctgaccag gcccctcgca gacaatcagg
    15001 tcctgaaggt caatttcgtc gatgtccagc agggcgacgg ttcggtgatc gaatcaccgg
    15061 acgggaagat catcctggtc gatgggggag acaaccagat gttcgcgcgg tacctggccg
    15121 gacgatttcg agggaccacg gcagagaagc cccaacccat cgactgcatc ctcgtcaccc
    15181 atggggatgc ggatcatttc gccggccttc ccgagattct caagtcggaa acaaacaagg
    15241 tgaagcgcaa acgactcttc atgcagccga agcgggtcta tcacaacggc atcgtgaagc
    15301 gcccgagcaa gatcaacaac aagaccgttc ccgacaccaa gctgctgggg ccgaccaaaa
    15361 cggtcaacgg acaactctac ctcacgggcc tggaagacaa tctgctcgac gtggccgata
    15421 cggagatgaa cgagccgttt cgagaatgga aaaacacctt ggccacctac aacagccgga
    15481 gtgaagtagc gttccggcgt ctccagttcg gagacaacca ggcctttgag tttttcaatc
    15541 gtggcgatct gcgcatcgag accttggggc cgatgaccac caccgtgagc ggacaaccgg
    15601 cgctgaagtt tctcggggaa ccacccaaag gaccgaggat cgggcatgac tcgctgagcg
    15661 acagcgacga aggcttcacc ggccactctg cctcccacac catcaacggc cactcgatcg
    15721 ttttccgtct ggtctatggc ggcttcagct tcctgttttc aggcgatctc aacgatgaag
    15781 ccagccggac tctggcgcgg gaacacaacc ggggcaacct caacttacga tcggaagtgt
    15841 tcaaggtgcc gcatcatggg tcggctgatt tttccggcgc cttcattcaa gccgtctcgc
    15901 cgatcgtgag cgtcgtgtcg agtggggacg agagcgcgcg caaagaatac atccatccgc
    15961 gagccacact catgggagcc ctgggaaaat ggtcgcgagt gcccgaaccg ctcatcttcg
    16021 tgaccgagtt ggtcgcgttc tttgaggagg aaggactgtc ccgattacag gacgagaaga
    16081 aggccaagaa acgggggcca ttctacggat tcagccgcac cgcctatggc ctcgtgaaaa
    16141 ctcgcaccga cggcacacgc ctgttcgtct atacggacag cggaaacgtg cagatgaaag
    16201 aggcctacgc ctatgcgctt gattcatccg gggtgccgca accggctgaa gtcaaacgcg
    16261 cttgatcggc aggagagctc aaggctcccc tgacgagccg ctttctcccc ccacgagata
    16321 cgcttcacga acagggagag acggctattt tttctcctgt gaccccggat gtcacagtca
    16381 ccggttacac tagccggtga acgacaaaag gggagggcgg ccatgcgatc ttcaaggctt
    16441 ctagcggcac tgctgctttc ggcgtcggcc tcggccgcaa cagcctgggc agagccgggg
    16501 ttcgatgaaa agtaccagcg ggacttcaac atctttaacc ccatcaacca gtatcagccc
    16561 ggcaatccgc tcaatcccgc caatgcctac gaccccgcga atccattcaa cccgatcaac
    16621 cagtacgatc ccaacaaccc cgccaatccc atcaatcaat accgcccgga aaatccgctg
    16681 aacccgatca ataaatacaa tcccaatacg ccatttgcgc cgttaggtgg cccaggacag
    16741 aaacggtgag gcgactcatg tctttctcga tcccagcgaa agacgcttca cgaacgacga
    16801 gatacggttc ttggggaata cgtttcacct ttctcgattg acgtttcacg ccccagtccc
    16861 taggcaacaa ctagtcgctt tggccagtaa aaacgcgggt gattaaaaga ggggaaacgg
    16921 gtattctgcg gcggccaaac cagatcccga cgaacggaga gaatgcatgc agtggatcgc
    16981 cccatcggaa aaagacactg cgacagacgc gctggaaaag cgtctctggg atgcggccga
    17041 ccagctgcgg gcgaatagcg gcctcaacgc cgcgcaatat tccaccccgg tcctgggtct
    17101 catctttctc cgctttaccg atgtccgatt cgcgaagatt cgggccggtc tggagaagat
    17161 ggcctcgtcg tccaggcgtg gttcccgcgt ggacgatccg gcggcctacc atgccgaagg
    17221 ggtgctctat ctcaccccga acgcccgttt cgacaagctc ctgcatctgc cggagggcag
    17281 caatgccggc aagacaatca atgaagccat gcgcgacatt gaaaagcaca atccccagct
    17341 cgccggggtg ctgcccaaga catatgagat ctttaacagc acgctcctga aagaactcct
    17401 caagcgcgtg tccgagattc cggccaccat cgactatgac gccttcggcc gcatctacga
    17461 atacttcctc ggcgaattcg cccggacgga ggggcaaaag ggcggcgagt tctatacccc
    17521 ctccggtatc gtgcgtctcc tggtcgaagt cctggagccc taccacggtc gcatcctcga
    17581 tccggcctgc ggctccggcg gcatgttcgt tcagagcgcc cgcttcgtag ctgaacacaa
    17641 gaagaacccg tcttcagaac tcgccattca tggacaggag aaagtcgcgg ctaccgtcgc
    17701 cctctgccgc atgaacctgg ccatgcacgg gctcgaaggg gacatcaaag aagccatcac
    17761 ctactacgac gatctgcaca acagcacagg caagttcgac ttcgtcctcg ccaatccgcc
    17821 gttcaatgtg aacgcggtcg ataaggaacg gctggaagca gagatcggca aaggccgccg
    17881 ctatcccttc ggcctgccgc gcacagacaa cgccaactat ctctggattc agctcttcta
    17941 ttcgacgctc agcgggaacg gtcgggccgg gttcgtcatg gcgaacagcg cctccgatgc
    18001 gcggtcatcc gagcaggaga tccggaaaca gctcatcgaa gcccgcgcgg tggatgtgat
    18061 ggtcgcagtc gggccgaaca tgttttacac ggtaactttg ccatgtaccc tatggttctt
    18121 cgacaaagcc aaggcaaagt taccgtgtaa aacaccctca cccggttctg cgaaccaccc
    18181 tctcccaaag gcagagggtc gaggaggctt caagtttcaa ggcttggtgg atcaagcaag
    18241 ggagctacgc aagaaacaga cacccgccga agacgtgctg tggcagttgt tgcgtgaccg
    18301 acagtttgct gacctcaaat ttcgccgaca acaccagatc ggcgactaca tcgtcgattt
    18361 cttctgtccc aaatacgccc tggttgtaga gcttgatggt gcggttcatg aaacatctga
    18421 taggaagaaa aaagacacga agcgcgatac gtatcttcgt tctcttgggc acacagtact
    18481 ccgttttacc aatgcagggg tcttcgatga caccgaaggc gtgctcagag aaattgtttc
    18541 ctcgcgcccc tctccctctg ggagagggga gggtgagggt gaacaacgcg gtgcgcgtta
    18601 tagcgacacc gtgttgttca tagatgcccg ccatatctat cggcagatcg atcgagccca
    18661 ccgcgactgg agcgtaggcc agatcggctt cttggccaac gtggtgcggc tttatcgagg
    18721 agaggaaccg gactttacgc tcggcgggcc tgaggcagaa gccaagttga aagaagtctt
    18781 cggcaagaag ctgaagttcg ccgatgtgcc ggggctatgc aaaggcgcca cgatcaaaga
    18841 gattgaagcg cagggctgga gcctgaatcc aggccgctac gtcggcgtgg cgccgggcga
    18901 ggatgtgagt gacgaggact tcaaggaaca actcgaaacc ttgaacgagg aactcgaagc
    18961 tctcaacgct caggctcgtg agcttgagat aaccattgcc agaaacgtgg cggagattct
    19021 cgaagcctaa agatgacgtc tgcagtgatg ataagaacga agcttaagga cgtttgtgac
    19081 cggatcacag tcggccatgt tggtcccatg gcagaggaat atacggagga aggagtgccg
    19141 ttcctgcggt ctcaaaacat cacgcctttt tccctacact tgcacgacat caaatacgtt
    19201 ccgccaactt ttcatgaaaa gcttaaaaag tctgcccttc gacctggaga cgttgccgtc
    19261 gtgcgtactg gttatcccgg gacagcgtgc gttattccag aatctctgcc agaggcgaat
    19321 tgtgcagacc tagtagtaat cacgccgtct gaaaagctaa atccgtacta tctcgccgca
    19381 atcttcaatt cggcatgggg gaaatcctct gtcgcaggta atcttgttgg tgttgcacag
    19441 cagcatttta atgttggtgc ggccagagaa atggagattg cgctcccgtc gcgagcgatg
    19501 caggaccaaa tcgcgggcat cctatcggct tatgacgacc tgatcgagaa caatcagcgt
    19561 cgcatcaaga ttctagaaga gatggccaga tccctctacc gagagtggtt cgtccacttc
    19621 cgcttccccg gccatgacaa agtcaaaatg gtttcttccc ccctcggccc catccctcaa
    19681 ggttgggagg tgaaaccgtt ggaagaactt gcgacggtca caatggggct ttcgccaaaa
    19741 ggcgacacat acaatgaaga agaaaacggg acccccttgg tgaatggacc tgtggaattc
    19801 ggcgatcggt tcacgaagcg gatgaagtgg acaaccgaac ccaccaagtt ctgcaaagaa
    19861 ggcgacctcg ttgtctgcgt cagaggttcg acgaccggca aatacgtgaa gagtgacggc
    19921 gtgtatagcc ttggtcgcgg tgtttgcgga atcagtagca agtatcaatg cttcatcgag
    19981 ctgctctttg agaatgaact gccgacactt ctaggccaga ctagcggctc aaccttccca
    20041 agctggactg ggccacagct aaaatcccat cgtgtgctgt ctccaccacc cgaggtgagt
    20101 gcgcggttcg acgcgcttgt taagccgatg agtgccactg tacttaggta ttcacgccaa
    20161 attcaaaatc tccgccgcac ccgcgacctg ctgctcccac ggctactgtc gggaaacctt
    20221 gatcttaatt aagccatgcg cctcaaagaa gtcggcatca agaatttcag gggataccga
    20281 aatgaaacac gaatgccgat cgatcccaat gtaacgggaa ttactgggaa gaatgatgca
    20341 gggaaatcga gtcttttgga ggcgctcgat attttcttcg agggagggga aattgcctta
    20401 gaaaaggatg acttcaatgt caatgagccg gatggcatgg ttgagattcg ctgcatcttt
    20461 gacaacctgc catcagaaat agtgcttgat gaaaccaacc ggacgacgtt gcaaaccgag
    20521 cacttactta atagcgcggg cgatttggaa atactcaagc gttacaagcg aagcgcatta
    20581 aaagaccctt ccatattctt gattgccaat catcctactg ccacggcgtt tgatgatctc
    20641 cataccttga agatcactga tctcaagaag cgcgctagag aaaaaggggt tgctgatagc
    20701 gaggttgcag atggacgggc gagtgcttca tggcgccttg ctatttgggg gaaagcccct
    20761 gatttgcaga gggaacctcg cgaactcgaa attagcaagt ttgctggcga ctcgaaaaca
    20821 atccaagata agctcttcaa gcagttgccc ctattcgcat tgtttaaatc ggatcgcgag
    20881 agcaaggaca acgacccaca ggctaaaaat ccccttcaag aagccgtaaa gcaagctcaa
    20941 gccgaactga aggctcaaat tgagaccatt caggtagaga ttcagaatcg ggtccttgag
    21001 agagctcaaa agacacttga gaagctgagg gaaatggatc cgtcgctcgc gtcacaactt
    21061 gttccacgat tcaaaaaagc tccatcctgg acgtttgaat ttgctctcga tggagaggat
    21121 ggcatcccta ttaataaacg tggaagcggc gtccgacgcc ttatcttact caactttttc
    21181 cgcgcggagt ctgaaaggaa agtggccgat aagaacgccc cttcagtcat ctatgccttc
    21241 gaagaaccag agacgtcaca acatccctca aaccaggaaa tgttggtccg tgcgttggtt
    21301 caagttggtg ccaaagacaa ctgccaggcc cttgtaacga cccatgttcc cgcgctcgct
    21361 ggtcttttgc ccgtttcagg tctacgcctg gcagaaaaaa caccctccgg aacaacaatc
    21421 gaatatggga gtgataccgt catagatcgc atagctcaat cgctcggagt cttgatagac
    21481 ccacgagcga gtagagcgaa aggtcttttg ctcgtagaag ggccgtctga cgttgtgttt
    21541 ctccggcaca ccgccgagca attgaaagcc ggaggctaca tttcagcgac attggaagaa
    21601 cgaggactgt tgccggtttc cataggtgga tgtgatttgt tgaagcactg gattgccaag
    21661 cgaattgctg agcaattcaa cattccttgg gcgcttctcc tagattcaga ttcaggaact
    21721 cctgagaacg gaaagaatct cgcacaggtg caagcgctta aacagttggg caagaaagca
    21781 tacgtcacac gcaaacgaga gccagagaat tatattttgc ccgaagttgt cctcccgcat
    21841 atcacatgtg gtgtcaccgt tacatggact gatacaagcg atgcaaaaaa agatattggc
    21901 tctgcaacct caaagagcca aggagaagtt ttggagttct tttggactca gatgaatgcc
    21961 gaacagattc gtcgtgctga aaaatacatt gaagatggtc aggaaaagtt cgaactcact
    22021 gagattctat cggattttct aaccttgtca taatcgatta tggccaaagg cttctccgaa
    22081 gacttcctcg tcgaacagcc tgcaattcag ctcttctccg agatgggttg gcagacgctg
    22141 tcggctcttg aggaagtctt cggcgttggc cgcacgctgg gccgggagac gaaaggcgag
    22201 gcggtcttgg cccttcgctt gcgtgccgca ttggtgcggt tactaccgct acactttgag
    22261 taaatcctgt tcgcctacta aatctcatgc caccgcctga atcctatccc atcatcgctc
    22321 ttcgcaagga tgatgtgttg gccccggaag agatgggcag caagcggaag gggtgggtgc
    22381 aggtcgccgg tgactccgag cgctggctct tcaagtatgc ccggctcagt aacggccaag
    22441 tcaccggtga acattgggcc gagaaggtgg ccgccgaagt cgccggctta ctccatattc
    22501 cccatgcgag ggttgaactc gccaccctcg acggatctcc aggctgcatc agccggcgct
    22561 tccccgagct cgccaggccc ggcgccgaac tgattcacgg caatgactta ctggctggcg
    22621 cggggttcgg ctacgaccgc gccaagcagt ttcgtcagag cgaccacacc gtcgaaaata
    22681 tcctggctgc gatctcgcat gtcttctccg acccggcaga gcaggcaacg gcctttcaac
    22741 agatggccgg ttatctcgtg ctcgatgcca tcatcttgaa cacggaccgg caccatgaaa
    22801 actgggcgct cattcgtgtg atgcatcagg atggaagggt tacacacgaa atggcgccca
    22861 ccttcgatca tgcctcttcg cttggacgga atgagccacc ggacaaatta gtagcctggc
    22921 tgacggaacc ggggcggcca gagtggtacg cgggcagaaa ccgttgccag ggaggaatct
    22981 accttcgatc cgacgatgcc cacggagcca atcctctcaa gcttctggag ctggttgcgc
    23041 gcaaatggcc tgactatttt gcgccctggc ggccacaggt ggaaaatgtt caggaagagg
    23101 ccctttgtga tacagttgga cgggtgtcgg ctgggaccat ggttcaggcc cagcgtgagt
    23161 ttgcgaaggc gttgcttcga gtgactctcc gacgggtcaa agacattctg ttatgaaaac
    23221 tctattcctg gcctggcaag acaaacggct tcgtggtgac catgtggacg gcacccgagc
    23281 ctggtttcca atcggccggt tggatgccga acctgacgat gcacactacc ggtttgccta
    23341 cacccgtggc gtattgcagg cgaaggagaa ggcagggttc caaccgcttg acgcgttccc
    23401 gaagttcgat gctctgtacg aatcctccga gttgttcccg cttttccaga atcggctcat
    23461 cagcacgaaa cgtgaggatt acgccgagta tctgcagcgg ctcgatttgt ctccggagtg
    23521 cgcggacccg tttgaaattc tggcaatcag cgggggcggg cgacagacgg acaatttaga
    23581 agtgttccct aaaattcagg cccgccgcga cggcagcttt tcctgccgct tcttcttgca
    23641 cggctggcgc catgtgaacg ccccatcgca agatcggttg aatgggcttc ggccaggcga
    23701 accgttacag gtatcgatcg agttgaataa ccccgcgacc ggcctggcag tccagcttga
    23761 gacggccaat gattatttca tgttgggttg ggcgccgcgt tacctgattc aagacgtgct
    23821 gcgtgccgtt gcaggtggac cgagtaacat gaccgcacgc gttgtccagc tcaatccacc
    23881 gcctgctcct cacaatcagc gtgtgctcgt cgagcttgaa ggccgcctca ctgccggcta
    23941 tgaaccgatg agcagcgatg agtttcttcc actgcgttcc tagcggcgca tggcccacgc
    24001 ctactccgaa gaccagctcg tcgaacaacc cgccattact ctgcttgccc aactcggatg
    24061 gcacaccatc tcggcagtgg atgaggtctt aggacctcac agcacattag gccgcgagac
    24121 caaggctgaa tccgtcttac tcttgcgcct tcgtgcggcc ctctgccgtc tcaacccggc
    24181 attacccccc gaagcgctct caacagccat agatgagctg gctcgcgacc ggtcagcgat
    24241 gagcctcgtg gcggcgaatc gagaggtgta tggacttctc aaagatggga ttcctgtttc
    24301 cgttccagat catataggtg gcggacagaa aaccgagcgt gtccgggtga tcgattggga
    24361 gaatcctgct gcgaacgact ttttgctggt gagtcagttc agcgtgacgg ggtcactcta
    24421 cactcgtcgg cctgacctcg tcgggttcgt gaatggcttg cctctcgtcg tcatcgagct
    24481 gaagaagccg ggcgttcccg cccagcaggc gttcgacgac aacttgacct gctacaaagc
    24541 cgacattccc caactcttct ggttcaacgg cctgatgatc gcttccgatg gaacggaaag
    24601 ccatgtgggt tcgctcacgg ctgattggga gagattcttc gagtggaagc ggatcgaacg
    24661 tgaagacgaa ccccgccgcg tgtcgctcga agtgatgcta cgagggacct gcgagccttc
    24721 gcgcctgctc gatctcctcg aaaacttcag cctcttctcc gagcacaagg cggggctggt
    24781 caaagtgctg gggcagaatc accaattcct cggcgtcaac aatgccattg ccgcgacctt
    24841 ggccgctcgg aaacagggcc atgggcgggg aggcgtcttc tggcagacgc aggggtcggg
    24901 caagagcctc gcaatggtgt tcttcgccca gaagatcctc cgcaagattc ccggcaattg
    24961 gacctttgtg gtggtgacgg atcgtgtcga gctggacgac cagatcgcaa agacctttaa
    25021 ggcctgtggc gctgtcagcg aaacggaaag cgaattgtgc cacgcgcaga gtggcgcaca
    25081 actccggcaa ctccttggcg agaatcatcg ctatatcttc acgctcattc acaagttcca
    25141 gactcctgaa ctcctgtgcg accgctccga tgtcatcgtc ctgaccgacg aagcccaccg
    25201 gagccaatac gaccagctgg cgctgaacat gcgtgcggcc ttgcctcatg cgcttttcct
    25261 cgccttcacc ggcacgccct tgattgcagg tgaggagcgg acgcgcgagg tgttcggcga
    25321 ctatgtctcc atctatgact tccagcaatc ggttgaagac ggcgcgacgg tcccgctgtt
    25381 ctatgaaaac cggacgccgg aactccgctt ggagaatccg aacctcaacg acgatattta
    25441 taacctgatc gaagccgccg gcttggatga tgaccaagaa aaacgattgg aacgtgagct
    25501 gggccgacaa tatcagctgc tcacacgcga tgaacggctt gagaccattg cccaagatat
    25561 cgtccagcac ttcctcgggc gcggctttca gggcaaggca atggtcatat ccatcgacaa
    25621 ggcgacggcc ctgcggatgc acgacaaggt ccgggcgcag tggaagtccg aagagaaacg
    25681 agtcgagcag gagttgacca ggctgacaac ctacggggac aaggctgccg accccgatcg
    25741 catccgggaa ctccgcgctc gtttagaggt catccggacg accgacatgg cgctgatcgt
    25801 ctcggctgcc caaaacgaga tttcggatat gaagaagctg ggtctcgaca tcgcgccaca
    25861 ccggaagcgg atgaatgatg aggcgctgga tgagaagttc aaggaggcga aagacccgct
    25921 gcgccttgtt ttcgtctgcg cgatgtggct gaccggtttc gatgcgccaa gctgttcgac
    25981 gatttatctc gataagccca tgcggaacca cacgctgatg caaaccatcg cgcgagccaa
    26041 ccgcgtattt cccggcaaac acagcggcct catcgtggac tacgcgaacg tctttgcctc
    26101 gctggagaag gcgctggcga tctatgccaa aggccgtggt ggggagcagc ccatccggga
    26161 caagcagaag ctcgtggaaa gcttgcggca ggcaatcgcg gaagcagaag ccttctgcct
    26221 ggcgcaggga gtcagtttgg cagagattga gcaaactccg gctgggagtc tcgaccggct
    26281 gacgaagatt gccgaggcgg tcgaatgcct gatctctccc gatcccctcc gcaaggattt
    26341 tctcggtcac gagggttggg tgcggacgct cttccgggcg gtgaagcccg atccctccgc
    26401 gctggagtgt gcctcgcgcg tggcctgcct gacgaccata gccgaaacca tccgtgaacg
    26461 cacgggcgag ggaccggcag acatctcagc cgtgatggcc gacctgaata agattctgga
    26521 tgcttccgtg gctgccgatg gctttcacat tccccaggga aaatccggtc atggcgtgat
    26581 cgacctgaca aagatcgact tcgaagccct ggcaaaacgt tttggaaagt ccaaaaccag
    26641 gaatatcgac ctggagcaac tcaaggcggc gatccgttcc caactgaaca cactcgtccg
    26701 tctgaaccgg accagggccg actatctgac gaagtttgaa gagctaatcg aatcctacaa
    26761 cgcaggcagc cggaacatcg aagaactctt caaggaactc ctcgcgctca gccgtagttt
    26821 gaacgacgaa caggaacggc acgtcagaga gcgtctctcc gaagaggaac tcgtggtgtt
    26881 cgacatcctg acgcgcccgg cgccgcagtt gagtgtagaa gaacgtgctg aggtcaagaa
    26941 agtcgcccgg gacatgctgc agaagttaaa gcagcttctg gtgttgaact ggcggcagaa
    27001 ggcctcggcc cgctcgcaga tcaaactcac tatcgaggat cttttagatc agggccttcc
    27061 gcgtgcgtat acgccggact tgtatcagca gaaatgctcc gcagtgtttg agcacgtcta
    27121 cgaaacctat ggggatcggg gagcaagcat ctatccagca gggcggtgat tcttcagaga
    27181 cttttgggtc cgcactcccg ccccccctca ctcgcatcga tcttcctcca catcacgccg
    27241 ccgcagaagg cgatgaacgc agccacaagc aatgaggtcg ccctccctca gagattgccc
    27301 agccagcagg tcaggctcga agagatgggc gcgccttgac ttgcgcccag attcatcacg
    27361 aacggcaacc tgatcgaatg gcgcttcgag cggccggtca accgcacagc ctactttccg
    27421 tcactcacgc catcgctgcc gcttcgaaga ccggcgccag tcggtggaca tccctgtacg
    27481 gcctgccctg tacggcctgc ttgacagtgt gaggcagact gcctagcctg attgctgccc
    27541 cttgtacttt gcggctgacc aacttcgatt cccaccggag caagtgagac gtcgatgatc
    27601 ccggcgacgt ttttcagtcc ggtatccggg ggaattgatg gcagctggaa ggtccgaaga
    27661 gttttcgaac agggccaggc cctatccctc atcgactgcg tgcacgacgt ggacgcaacc
    27721 cggttacggc atccagcctc cgcggcctca tacacgtcgg attggaggaa tcctcacatg
    27781 agtcaccatg gcaagacgaa ggcgcagaaa ggctcgctgc cggccatgac cgtcacgccg
    27841 gttgctgcgg cgatcggcgt ggtgctgcga gacgaccagg ttctactcgt ccgccgcgcg
    27901 aatcagccgg acgcggggaa gtggggcttt cccggtggca agatcgagcc gggtgaaccg
    27961 gtccaagctg ccgcggcacg ggaggtggcg gaagagaccg gcctcgtcgt gcgcccgctg
    28021 cacgtgttca cggcggtcga cgccttccat cggaacgcga gtggacagtt ggtcacccac
    28081 tatgtgttga tcgccgtatt atgcgagtgg atttcaggcc tcccgcaggc ggccggtgat
    28141 gcgttggagg cggagtggtt cccgatcgag acgttagact cagtcgacat cgtcatgagt
    28201 gttgacgtcg cacgcgtcgc ctcgatggct gcgaaggtcc gtgacgacgt tcaatgaccc
    28261 gctgttgatg aaggatcctg aatcgggcag gcttcacagg cttctgtggt tgcgccgatt
    28321 ccgctagcac ctttggatcg accctttgtc cacggagtga cttccaaaac cagtcagctg
    28381 aaccgctggt ccccggcttc gtccaacttc gagcgttctc cttcatgagc ccgtcacttc
    28441 tacttcttca tcgcctgggg cgaatcaatc ttcacccaca tcacgtcgcc gcaggtggag
    28501 atgagtgcgg ccaccagcaa cgaggcggtt caaccccagc tatcggcgag ccagggcgtg
    28561 agtgtgggac aagtaggact gtcgaaggat tacggggcaa agagttcggc aagcagagtg
    28621 aggatctggg cgcgtgtcac gtcagtgatc gttcctacac gcttgaccaa tcggcttttg
    28681 tccactgtcc ggatttgatc cagcacaacc tgcccggact tacctttgaa tcgaagcgag
    28741 acacgagtcg ggtaggggcg gcctttcgtc gtcatgggag caatgatgac cgtgtcgatg
    28801 tgctgattca tttcatccgg ggaaatgatg agacaggggc gggtcttacg gatctcacgt
    28861 ccttgtgtag gatcgagacg gacgagaaag acatcgaagc gggacactac catgtccatt
    28921 cgctccgatc ccatgtagac atcgctgcag attcttcatc cagcaggtgg tcatccccgt
    28981 gtcgatgcat ctgctcaaac gcttgacgcc agccggcgcg cggtttggat gccgcacgaa
    29041 taaccaattg tccgtgctga atctccagat cgacgtctcc gtgcaggtga cactgttcaa
    29101 tgacagattt cggaatccgg atgccctggg aattgccgat tcgcacaaca cgagctttca
    29161 ttgcgatttc ctcccctgcc ttgctgaact cttgtgtaat tacattggca ctacccgctc
    29221 tgtcaagaat tcataccacc gcctcactcc ctcaacccct cgctcgcatc gatcttcatc
    29281 cacatcacgc cgccaccagc aacgaggcgg tccaacccca gtggtcggcg aaccagggcg
    29341 tgaggggcag ggaaaatgag acgtaaaaat gtacagggag tctcctaggc cccccatgtg
    29401 ctgcaacatc aggaaatggc tggtatggcg caggagatag aacgggtgct ccagccgccg
    29461 acagaagtgc ggctttcacg gttcgaccgg gcggtgcggt tattttatga gcgctacgcc
    29521 aagacgaagg atggtgggaa gtggctctga gtcgtggtaa cctggaaagc ctgggcaagg
    29581 acaacggcga aggggagaca ggaagccata cctcccgctg ctgcccgacg atgagcctac
    29641 ccgcattatt ccgccttcca tcccgagtgc atcggggcta tgatcgccgc atgccttcca
    29701 tggtcccccc atttaccgcc acccaggccg ccgtcctgaa gaactttctc gcatcgcccc
    29761 agcgcccgac cggcacgctg acgtatccac agctggcagg atttctgttc agcctcgcga
    29821 acggtccgga attaatcccg ccatcggagt ggataccgat ggtattcgat gatcatgacg
    29881 ccggctacga cacacaagct gaggcagagc aggtgcttca ggccatgatg gcactctata
    29941 acgactgtgc ccggcagtat tttgctgatc gtcccacgct tccacctggc tgcgacatca
    30001 gaccggaccc cctcgataat ctggagcccg acgcgccgct gagccactgg gcacaggggt
    30061 tcggcatggg ccatgactac ctggtggaat actgggacga attcaccccg gaggaactcg
    30121 atgaagcgct gggagccgtg ctgatgacgt tgaccttttt ttcctctgcc agcctggcca
    30181 gggcctacca tgaagaaggc aaggccggca caagcctcgc gcagttggcc ggaaccgtcc
    30241 tcgacatctt tcacgatgcc ctgggtgagt acgcgcacct cggtcgcgca atctatcagg
    30301 gacggtgcga agcgggcgat ctcagccctg caccgacgac cggccggaaa gtcgggcgca
    30361 acgatccctg tccatgcggc agcggcagca agttcaagaa atgctgcggg gcgacatgac
    30421 tcagtgccgg gacgtatcct cgggtgcgga aacaccaccg gggcatctcg tgggcaggtg
    30481 cctcttcacg gcgatactcc gcaagcttgc gaccggtctc gtctcctttc actccctcac
    30541 ccctccgtcg gatcgacctt catccactag cgacaccctc caaccccagt gatcggcgag
    30601 ccagggcgtg aaagtatgaa cactggcggc gcttcacttc accacagagc acagcctaat
    30661 ggtcggggag agcgagccgg ggaactaacc gagatccatt acttctttga gagcgtgggt
    30721 tatggccctg agttggatcg tggtgaggcg ggtcaacgga ccttcgatca atcgttggtt
    30781 atcgatagca cggagttggt ccaacagcag atcagagtct tttcgaaatt tgctctgggc
    30841 ggtaattatg agacggagcg gttccgatgc ctgatcttgc ctggatccgg cccacggcga
    30901 ggattcaagt cggcaagccg aatctcgccg cgcttacgca taaaggtctg tctcaatggc
    30961 gtcaaactcc ccgttgatgc gcatactttg gtcgcgcacc ttatacgagg cctgggtgag
    31021 acgatcgaca cgaagctgtc gctcggtttc gaggttcatg cgttccaagg attgccggat
    31081 ataagccgcc ctcgatatgt ggagggcctg ggcgtaacgc gtgcaacgat tgagcaattc
    31141 gtccggcaat gtcactgtaa tggtactcat cgatacctcc agtaactatc ggaacccgat
    31201 atcatgtcat actaaatcga ggatgagcga cggcacaaac agtcccgcat ccgcgttcgt
    31261 tatgcgccac gccagtctcg cgccaccgcc tcactccctc aacccctcgc tcgcatcgat
    31321 cttcatccac atcacgccgc cacagagagc gatgagcgcg gccacgagta acgaagccgt
    31381 ccaaccccag tggtcggcga gccagggcgt aaggctcgga gaaatgacgc cgcctacgtt
    31441 cgcgcccata ttcatcacac ccgacagggt gcctgcatgg gtcttggaga gatcggtcgt
    31501 cacgctccag taggcgccga cggtaaaata caaccagccg gcgcccagcg agagggaggc
    31561 gatggccacg gcctgtgagt cggcccaggc gccgacggcg atcagtccac cggccaaccc
    31621 catgccgatc atgccgacga tcattcgagc cttcgtcaga ccgagggtcg agaccaggcg
    31681 atccgtggtc cagccgccga gcgggcagaa gacgaggatc gccacgaaag gtgcagccgc
    31741 aagccagccg ccacgcaaca gatcgatccc gcgcacattt accaggtaga gataaaacca
    31801 ggacatataa atgtaggcga cgtaacccag gcaggcgtaa ctcaacacca gccagcgcag
    31861 ggatgacgaa gcggcgaatt gccgccaggg aacgactacg cgcgggtgta cggacgccac
    31921 gccctccgcc gctttctgct ctcgggagca gaacacccat aacagggcca ccaccagacc
    31981 gatcaacgcg gagaggtaga agaccgtctg ccagtggtaa ttcaccatga cccaggaggc
    32041 gaggggaggg gtgatcgcgg cgccgatgcc gatgccgccg atggcaatgc cgatgccgag
    32101 tccacgttgc gcaggcggaa tccaatcggc cacggcccga ttgaaattcg gcagagccac
    32161 cgcttctccg acaccgagga gaaagcgcac gacgatcagc gcaccgaccg tcccgaccat
    32221 ggcggccaac ggcagggttg cggccaccgc cgtcaaggcc gtgaacaacg accaccagac
    32281 cagcgcaccc gccagcacca cccgggctcc ccagcgatcg cccagccagc cgcctggaat
    32341 ctggcagagc gcgtaaccga acacaaaggc ggaaaagata tagcccatgt cctggtcggt
    32401 gaggccatag gcgggcatca tctgtcgcgc ggtgacggag atgttgaccc ggtccatgta
    32461 ggtcacggcg ctgatcgcga acaggagccc gagaatgccc catcgcggtt gcgtcgtcgc
    32521 tctgcctgtt cccacgcgta cccccggttt ttactcaccc gtcagaaagc gaggcattat
    32581 agaccggcac gcaccagccg acgaaaggta tttgcgatcc ccaacgagat acggtctcct
    32641 accgtttcac gtctcacgtg tgacgcctca cgtttcacct ttctcgtttc acgtttcacg
    32701 cccgatagcg cctctctgcc gtatggattt ccctctcatg catccagtac actcagattt
    32761 tgacctacct gatggaggtg gagtgagggt tcgtccgcgc gttgtcgtgt tatcgctgct
    32821 cggcctggtg g
//
LOCUS       DAORRQ010000046        32253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_81_length_32253_cov_75.277781, whole genome shotgun sequence.
ACCESSION   DAORRQ010000046 DAORRQ010000000
VERSION     DAORRQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 32253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32253
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81_length_32253_cov_75.277781"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /gene="hisC"
                     /locus_tag="PK224_14240"
     CDS             complement(<1..921)
                     /gene="hisC"
                     /locus_tag="PK224_14240"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRB16952.1"
                     /translation="MPLKVHPDIASLVPYVPGKPIEELQRELGLPRAIKLASNENPIG
                     PSPKALAVLGETAPTLHRYPDGGAFRLRGALAERWKVTPDHVILGNGSDEILGLLART
                     FLSPGDEAVMAEHTFVIYKMEVKAAHGVAVEVPQKNWHHDLPAMAAAITDKTRLLFVC
                     NPNNPTGTMATKAEVAALMARVPDHVVVVFDEAYYEYVRHPEFPESLSYVNAGRNVIV
                     LRTFSKIYGLAGLRIGYGMTTPEITNYLNRIRPPFNANSMAQRAALAALDDEAHVGAS
                     RALNHAEMDKVRTGLLTLGFEALPSETNFLY"
     gene            complement(938..2014)
                     /gene="pheA"
                     /locus_tag="PK224_14245"
     CDS             complement(938..2014)
                     /gene="pheA"
                     /locus_tag="PK224_14245"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRB16953.1"
                     /translation="MSDDLQGHRQEIDRIDDQILRLLNERSKSVIEIGKLKKQRDAEA
                     HLHTPAREAAIFERLSKQNTGPFPTDAIRAVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACMQKFGSSAQYIPVNSIKDVFSEVERGRAHFGVVPIENTTEGVVNHTLDMFVDSS
                     LLIYGEVLQEVAHHLMSKSGVAGEIKRIYSHPHAIAQCRNWLETNLPHVPVSEVASTA
                     RAAELSVDDPSAAAIASELASQLYGLKVITARIEDNINNFTRFLVLSQKAPERTGRDK
                     TSLMLSVKDKVGALYDLLRPFASHGLNMTKIESRPSRRKAWEYIFFVDIEGHIDEERV
                     KKAAEEVKSRCLFMKILGSYPAYS"
     gene            complement(2154..3191)
                     /gene="ruvB"
                     /locus_tag="PK224_14250"
     CDS             complement(2154..3191)
                     /gene="ruvB"
                     /locus_tag="PK224_14250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRB16954.1"
                     /translation="MSDRTVSNQLTDDERGLEAALRPHSLQEYVGQSRMKESLEICIE
                     AAKRRGEALDHAIFYGPPGLGKTTIAHIIAREMGSAIRSTSGLVLSHAGDLAAILTNL
                     QERDVLFIDEIHRLPASVEEALYPAMEDYQLDLVVGQGASTRTVKLELPRFTLVGATT
                     RAGALTSPLRDRFGLVHRLEFYSSQELTSIVTRSAGLLNIPIDEAGAAEIARRARGTP
                     RIVNRLIKRIRDYAEIKAGGRITKQVAQDALVWLAVDAAGLDEMDRRILLTVMEKFNG
                     GPVGVDSLAAAVQEDRGTLEDVYEPYLIQAGFLERTGRGRQATRLAFDHFKKQKDLLS
                     LSDDDTSVTTP"
     gene            complement(3222..3728)
                     /locus_tag="PK224_14255"
     CDS             complement(3222..3728)
                     /locus_tag="PK224_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16955.1"
                     /translation="MMKSLSRWWGSRRLVGTVRLVIMAAMVSSGSAGLVGAADGVEEP
                     KSIRKTCGMCPEGYATTGVTHAPELCKDGEPTLVQCVPLGGNMLSVCGTCPEEYVEVG
                     RSNVPSRCGSVDGGLVSQCQLQNMGSTMPDPGQGGVRCPPDCGSTAAPGQGAVSPPPK
                     FRPSPEAR"
     gene            complement(3732..4340)
                     /gene="ruvA"
                     /locus_tag="PK224_14260"
     CDS             complement(3732..4340)
                     /gene="ruvA"
                     /locus_tag="PK224_14260"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRB16956.1"
                     /translation="MIALLTGLMAFKAPSHVTLDVHGVGYEVLIPLSTYYSLPNQHEL
                     VTLSIHTHVREDAIQLYGFLTAAEKEAFLLLTGISGIGPKLGLSVLSTLSVRDLFSAI
                     QVGDIEKLGTVSGIGKKSAARIALELKDKVARLHPAGEPAEPGSGRSANPLYEDALSA
                     LVNLGYRQPDVKEALKRIEKSGAMAQGLEGVIREALKDLAKG"
     gene            complement(4422..5177)
                     /locus_tag="PK224_14265"
     CDS             complement(4422..5177)
                     /locus_tag="PK224_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRB16957.1"
                     /translation="MGGHSHWATIKRHKSAVDAKRGKVFTRIIRELTIAARSGGDPDG
                     NPRLRLAIAKAKEANMPGDNMKKAIQRGTGELPGVTYEEFTLEGYGPGGTAVLMEITS
                     DNRNRTVAEIRNLLTKNGGNMAEAGAVAWQFHKKGVLVVEKGKVEEDALLSLALEAGA
                     EDVKVTDKAFEVLTDTHDFEAVKKALSDAKIECTLAELNFIPQNTIALEEKAAEQMLK
                     LMEILDEHDDVQKVYANFDISDEVMEKVAAATA"
     gene            complement(5317..6159)
                     /locus_tag="PK224_14270"
     CDS             complement(5317..6159)
                     /locus_tag="PK224_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16958.1"
                     /translation="MRETSARPIRQSGWAFQFLPVFFSRPIHCPRFLMPWFPFLGMLC
                     LAALLWSGSFVMAGNADHGHDAAVQEQGHQHAAVRGELWEGSRQGIAYSEFNHHMAGV
                     FLLLIGLSEVRQALGWPALVWTRFLLPGALTVGGIFLLIWSDHDSWPIGSMTFAQTFF
                     GSDEEVLQHKTYGVLAFAVGVIEFLRRTGWLAHAVWTVPLPLFAIVGGLMLFSHSHGD
                     HPAASTIVLHHVIMGMMAITAGSSKLVSGWGTRSVLTERSYWELLWASLVVFIGLQLL
                     IYSE"
     gene            complement(6171..6893)
                     /locus_tag="PK224_14275"
     CDS             complement(6171..6893)
                     /locus_tag="PK224_14275"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HRB16959.1"
                     /translation="MTFQKVEKPGEGSLGVFFGCPSCEAKFSMVTNPGETQMVASLGV
                     KLGGRTETAEPFEMTRGTLKEEAQAGAGQMAAYLNEKIQGGQPVAASAKPSAPDAGGE
                     KTSGGCPFSAMVAEMGLTSGGKPQNGNGTSEFTWTADAKEKLERLPSFVKPMVQSSVE
                     AYARKHGYKSITLQVMDDSKNDSPNGIAWTKDAEQRMDNIPDFIRPMARREIERIAKE
                     RGLVEITAQVMDEAKEKFMKFM"
     gene            complement(6953..7879)
                     /locus_tag="PK224_14280"
     CDS             complement(6953..7879)
                     /locus_tag="PK224_14280"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRB16960.1"
                     /translation="MTMAAGKDLKTILTKLQYSDDAKVVQQITAQMKQVQARMAGIKQ
                     KLVVMSGKGGVGKSMTTVNLALALARQGAKVGLLDVDLNGPCVPRMMGLHGQSLRMTP
                     EGAQPPVGPLGIKVASMDFFLDDASPVRWKGPMDLSPVWLGLMEMNVIREFLADVVWG
                     ELDYLLADLPPGAAADKPPVIAGFIPDLAGAIVVTTPSEVASDVVQKSVTYARDMGIR
                     VLGVVENMSEYRCPSCGAENELFEGNTEAMCEVLDLPLLGRIPFDRKFAKTFDKGQPL
                     LDPEYPTIQKYQEIVGRIQALLDYKKVLAEKL"
     gene            complement(7957..9804)
                     /locus_tag="PK224_14285"
     CDS             complement(7957..9804)
                     /locus_tag="PK224_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRB16961.1"
                     /translation="MTQHDQSKPQESLNIVIVGHVDHGKSTLVGRLYADTGSLPEGKV
                     EKVQAICRQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFIWNGRQYIIIDAPGHKEF
                     LKNMISGAARAEAALLLIDALEGVREQSRKHGYLLSLLGVTQFAVVVNKMDLVGYRQD
                     VFDGIEKEYREFLGQFRAVPQQMIPVSAKMGDNIATRSSHMNWYQGPTVLETLALFKK
                     EQVRSEQPLRFPLQDVYKFDARRILAGRITAGRLKVGDQLVFSPSNKTAIVQSIEGFN
                     VDPPRGEAQAGQSVGITLDEQIFVERGEIASHRESIPLVSTAVRVNLFWMGRRPLEKG
                     RKYVLRLATREVACEVAAIHRIIDTADLAQLQESQAVAKNQVAELTLRVKAPLAFDLS
                     SSFEATGRFVLVDEYDIAGGGIITELVHDEQEGLREEARQREYAWLTGDVRAEDRATQ
                     YGHRAAIVLFTGSAQTGKTFLARRVEALLVADSRHAYLLEGENLLQGLDADLSAADPS
                     FAAERVRRYGEVARLLIDTGLIVVSTSKTFGINYQRMAEMIRTLVQPAPVIAVHMSRA
                     GEEPPPNTDLHFAGPQDFDAAARQILEELKRRGVLIQPSGTKSTIQYSI"
     gene            complement(9888..10685)
                     /gene="cysD"
                     /locus_tag="PK224_14290"
     CDS             complement(9888..10685)
                     /gene="cysD"
                     /locus_tag="PK224_14290"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRB16962.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFNHLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSYKIPAMIEYRDRIAREWRLNLVVGQNKEALAAGMNHTLGRDV
                     CCTALKTTAMKNLVEEKGYTGIILGVRADEDSTRAKERYFSPRDKHGDWDFRDQPPEL
                     WDQFKTTFPPGTHIRIHPLLDWTEINIWEYIKHENIPFMDLYLDRGDGTRYRSLGCAP
                     CTMPIKSTAKTVDEIIAELRGTNVAERAGRAQDAGRGMEMLRKKGYM"
     gene            complement(10703..11770)
                     /locus_tag="PK224_14295"
     CDS             complement(10703..11770)
                     /locus_tag="PK224_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRB16963.1"
                     /translation="MQHLLAKVAKGQKTSKDLTWEEAKQAMRLMIEGTATPAQIGAFL
                     TAMRFKSESVTELAAFTATARQYVPPVPVRSGLGVVDVPVYAGKRETFHAILPAAIVA
                     AAAGAVLLLHGVDGPPDRLGISSVLKLLGIPVDMTAKSVGAELEKKGFAYLDLALYHP
                     PVSRFLEMRQELGVRNFFHPVARMLNPARATSQVIGLSHPPYFEKTIEALRMLSCPRA
                     LVIRGVEGDPELSIGNSTRLLELKDERITPFTFQPKDAGLSMATFREMAGFPVEQRER
                     EADLIKRLLANEIQGGQRDWVLLNAAMLLYAAGKGTSITGNLGAARSALESGQAKAKL
                     AELVAVAGSSAGNAPKVGISA"
     gene            complement(11812..12534)
                     /locus_tag="PK224_14300"
     CDS             complement(11812..12534)
                     /locus_tag="PK224_14300"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRB16964.1"
                     /translation="MKDTQKPAARPSDAELKALSDSFESQQPWELLEYALKTYRQRIV
                     LACSFGAEDVALVDMVHRIDPDAPLFYLDTDFLFPETFDVRDRIIARYGLKPAQVIQM
                     KSLLTPEQQAAQHGTMLWASQPDQCCELRKIEPLTRILGEYSAWITGIRRDQSPARAN
                     AGLIEWDKKFNLVKVNPLARWSSENVWTYLQLHEVPYNTLHDRNYPSIGCTHCTAPVL
                     PGDDPRSGRWKNFGKTECGLHK"
     gene            complement(12540..15035)
                     /locus_tag="PK224_14305"
     CDS             complement(12540..15035)
                     /locus_tag="PK224_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRB16965.1"
                     /translation="MTPDTVALRPAIKTEPIPAHIVEEIETFDAEAQRVLGGDLSTDI
                     FKPFRLQYGIYGQRQPGVQMVRIKIPFGGLTGNQLRQVADLADRYATGVGHVTTRQDI
                     QLHFVELKHVPEMMRLMASVGLTTREACANTVRNVTACHLAGVCQGEVFDVTPYAKTV
                     AYHLLRNPLNQSLPRKFKIALSGCRQDCALTPIHDIGLLAAKRADGTIGFRMVAGGGL
                     GSTPRMAQVLREFTPMDELLPTIEAVIKVFDTLGNRKNRNKARMKFVIEKLGFDEFKR
                     RWEAAYAAMGYAVPTHEPITLLEYADAPPLIMPTKAGVLSNGHGNGSGGGAVSLNGQV
                     SAFQAWRRTNVVPQRQAGFVTAAIKLPMGDLTGDQMWVVADLAERYSNGNIRTTINQN
                     MVIRWIPEGRLEEFYQELVQHSLGDPGAELVEDIIACPGTDTCGLGITSSKGMARALA
                     EVFPAGQVPEDLRDVSVKISGCHNSCAQHHIATIGLHGVGKRLGEHTAPHYELHLGGH
                     VDGTPKIGQLAVKLPAKNVPAAVRHLVDVYRRDRKSGESLQLFIARVGKNVLKDELIP
                     YTIVPPYEQDSTYYYDWEGEAEFVLEDLGPGECAGGALEMIDDRMLEADQELYQAKLL
                     VEKHQYALSVNKSYRAVLAAAKGLLVTEGLDPATDAETFQEFDQRLASKGIVPASYKN
                     LGAQVGDLGSKDATAEAATEKMAFAKRFLAVCRAATEQMGKDLKLAQVKEEAVPTAAP
                     AAVPASPAPVAVAQAPVYDLRGVACPMNYVKTKLKLEMMDNGEQLEVWLDAGEPIRNV
                     PMSLRNDGHRILDEGPLEPEAKHFKILVEKVEG"
     gene            complement(15090..15542)
                     /locus_tag="PK224_14310"
     CDS             complement(15090..15542)
                     /locus_tag="PK224_14310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRB16966.1"
                     /translation="MKVSLRATYGIIAAVDLALHHAEQPVCAKSIAKRQAIPARFLEQ
                     VLHAMKKAGVVTSQRGAQGGYVLSRKPSELSVADILDALEGPLLATNGEAGLKHSSSR
                     GAKQEALLAHIWDRVKRAELSVLSEVTVEELAKRQRALDAQHTLMYHI"
     gene            15883..16473
                     /locus_tag="PK224_14315"
     CDS             15883..16473
                     /locus_tag="PK224_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB16967.1"
                     /translation="MTLRSSTFNILLLSTDGEIQAHYKDLFGEQAVTIGRDGLSLPKE
                     PAKRAYDAVIVESKAQSAADLGKLFGGIDPARTLLVVGSRTVLKRTATSLHAAKAPKA
                     DLSLSNGNAPGQSVCLESFLEHKVGDFVKGMRNGSGRNLHPMLIAAVERPLILLTLRE
                     TKGNQIQAAELLGLNRNTLRKKILDLDIPVKRARAS"
     gene            16531..16842
                     /locus_tag="PK224_14320"
     CDS             16531..16842
                     /locus_tag="PK224_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRB16968.1"
                     /translation="MYPPVANHQRREAMTKEELIAKMANSAGITKVAATVALEAFTGA
                     VTTSLKKGKRVTLVNFGTFTISKRKARMGRNPRTGEALKIPAARIPKFSAGKELKAAV
                     K"
     gene            16977..17051
                     /locus_tag="PK224_14325"
     tRNA            16977..17051
                     /locus_tag="PK224_14325"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17009..17011,aa:Val,seq:gac)
     gene            17138..19093
                     /gene="thrS"
                     /locus_tag="PK224_14330"
     CDS             17138..19093
                     /gene="thrS"
                     /locus_tag="PK224_14330"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRB16969.1"
                     /translation="MASQFIHITLPDGTRKQVPAGCTVREALTPEGGRLDAKVLAAKI
                     NGEPMDLSRPLEQDATIEPLTFESAEGREVYRHSSTHIMAQAVKEVFPTAQLTIGPAL
                     DDGFYYDFAFDRPFTPEDLEKIEARAIEIMKRGLTVTRSELSKQDAIKFFQERGEGYK
                     VELINSFDDASPISLYRQGEFVDLCRGPHLPTTGYVGAFKLLSTGGAYWRGDERNPML
                     QRVYGTSFPTKKELDAHLAKLEEIKRRDHRKLGKELDLITIQDEIGPGLVLWHPKGSL
                     IRLLIENFWREQHIKDGYDLVYSPHVARLDLWKTSGHVDYYRENMFASMKLEGSEYQL
                     KPMNCPFHIMIYKSHMRSYRDLPIRYGELGTVYRYERTGVLHGLLRVRGFTQDDAHLF
                     CRPDQIEAEVSRVLDFTFFVLGTFGFTEFEVYLSTRPEKSVGSEENWTIATNALEAAL
                     KSRGVAYEVDPGEGVFYGPKIDIKIKDVLGRSWQCSTVQVDFNNPERFKLAYTGEDGK
                     AHQPIMIHRALMGSIERFFGILIEHYAGAFPTWLAPVQAVVLTITDNQQEFAAKIVST
                     LKSHGFRVEADIRNEKIGFKIREAEKNKIPYMLVVGDKEVQSGMVSVRGRSGANHGSL
                     PIEAFLELLRTDANQTLRQTATHSQTR"
     gene            19097..19603
                     /gene="infC"
                     /locus_tag="PK224_14335"
     CDS             19097..19603
                     /gene="infC"
                     /locus_tag="PK224_14335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRB16970.1"
                     /translation="MVPKLRVNREIRIREVRVIGPEGEQLGILPTVEAFNKAQEGGYD
                     LVEVAPTSQPPVCRIMDYGKYKFELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIK
                     IRQIKEFLADGNKTKVTLTYRGREMANQEMGRTMMASVIKECTEAGTVEFAPRMEGRS
                     LIMILAPK"
     gene            19643..19834
                     /gene="rpmI"
                     /locus_tag="PK224_14340"
     CDS             19643..19834
                     /gene="rpmI"
                     /locus_tag="PK224_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003851299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRB16971.1"
                     /translation="MKMKTHSGAKKRFRRTGTGKLVRRKAGGRHLLTGKPRDRKRNLK
                     GAVEVSSASTPALNRIIPQ"
     gene            19880..20248
                     /gene="rplT"
                     /locus_tag="PK224_14345"
     CDS             19880..20248
                     /gene="rplT"
                     /locus_tag="PK224_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRB16972.1"
                     /translation="MPRTKGGPKTRQRRKKRLKLAKGQYGAKSRLFRTATESVDKGQA
                     YAYVGRKNRKRDFRQLWIARISAATRLHGIAYSRFMNALKKANILLDRKVLSDMAIRD
                     MAGFEKLVGVAKQQLATVAS"
     gene            complement(20308..22026)
                     /gene="pheT"
                     /locus_tag="PK224_14350"
     CDS             complement(20308..22026)
                     /gene="pheT"
                     /locus_tag="PK224_14350"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRB16973.1"
                     /translation="MPTISLQRDDLEALIAGPDEEPARIPLDQLEQWLMLVKGELKGH
                     NSETGELRIELQDSNRPDLWCCEGIARQIRIKQRGSAISYPFFKKPKGVAGKLVVAQG
                     MDQIRPYVAACTAVGYRVTASGLTQLIQTQEKLADIFGRKRRSVSIGLYRLAPIVFPV
                     SYDLVKPDDVRFTPLGMETMMTLREILMVHPKGVEYGGIVGGHDRLPVLRDAKGQVLS
                     FPPIINSREIGEVQVGDHELFVEVTGTDLSMVALTLNIFAANLADRGAVITPVEIHSP
                     VKTNFGRRWSTPIDFGESRTIPLKAIESALGEALGGKEVASALKSYGYTVKSAGQKVA
                     VQLPPYRNDLLHTVDVVEDVAISQGYARFAPVMPSQFTVGGLSRLEQMADRVRHLMVG
                     LEFQEIISNIMGSHQDFCTRMRLDGTEWAKVVEVDNVMSLSYSCLRQWITPSLLQVET
                     NSSRAFYPHRMFEVGEVAIPDASADVGSRTVTMLGAVIAHAGAHFSEIHSCLDLLLYY
                     LDRPFTLEPVDHPSFLDGRAGKIVSNGRVIGLLGELHPEVLEHWQIGVPAVALEVEID
                     RLLEEV"
     gene            complement(22039..23583)
                     /locus_tag="PK224_14355"
     CDS             complement(22039..23583)
                     /locus_tag="PK224_14355"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRB16974.1"
                     /translation="MDNLHPLESKVLLALTRQPDAPPTLDQLAASTGLEPSQLSMAVE
                     WLLAKSLIAVQAETVAHIASLTPIGELFFEKYAPIERVLSAAREAGQTGKRLTIQDIQ
                     AKEALEPSDVSKAVGTLKKEGAILIVQGGCIESTGRNSATAEAMRSLLQELRSGQREL
                     QSFSEPLRSVLQQHAVKRGNAREPFRIDERVTRSFVLTPAGQEVAEQLSRDGVAEEVS
                     QLTPELLKEGAWRTKRFRKYTISLRAPRIAAGKRHPYREFLDLVKLKLVSMGFQEMRG
                     TLVETEFWNMDALFMPQFHPARDIHDVYFVKNPTHARTIAEPYLTQVTQVHEKGTGAG
                     STGWGYAFDAERAKRLVLRSQGTAVSARTLAAGASVPGKYFSIARCFRYDQVDATHAT
                     DFFQVEGIVLGADINFRTLLGLLNLFAREVAQAKEVKFLPAYFPFTEPSVEMHVRHPK
                     LGWMELGGAGLFRPEVTTPLGVSVPVIAWGLGLDRMAMVALGIHDIRDLFSADLEFIR
                     TMRGSF"
     gene            complement(23611..24315)
                     /gene="rpe"
                     /locus_tag="PK224_14360"
     CDS             complement(23611..24315)
                     /gene="rpe"
                     /locus_tag="PK224_14360"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRB16975.1"
                     /translation="MAGRTVRIAPSILSADFARLAEEVARVEDAGADWLHIDVMDGHF
                     VPNLTVGPPIVEALRKVTTLPLDVHLMMTNPDAFIGEFAEAGADYLTVHVETCPHLHR
                     TVQSIKERGVKAGVTLNPATPAGTLSEIVRDADLILIMSVNPGFGGQKFIASSLRKIA
                     EVRALIDRTGSRALLEVDGGVKPDNVQEILAAGAEVLVAGSAVFSTDDYAAAITALRA
                     GHEPAARTARVATAQR"
     gene            complement(24315..25727)
                     /gene="rsmB"
                     /locus_tag="PK224_14365"
     CDS             complement(24315..25727)
                     /gene="rsmB"
                     /locus_tag="PK224_14365"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRB16976.1"
                     /translation="MASDLPPSKSRGIVSAGRRAAMKALLAIDTAGMLGDDLFDQVST
                     REALDLRDRAFMVELVRGVLRYRTTLDWRLGLLSDRRITKLPTLVQTILRLGAYQLLY
                     LDRVPDSAAVNESVQMTKQQSRRLGRDWSGFVNAVLRALLRSPEPAWPDAGSDPVVAL
                     AVRYSCPAWLVERWCHRLGAERAEVLCRASVEVPPLTLRANTLRTSRAALSADLAAAQ
                     VEAGPTSISEVGIQLARSCSVIDLPGYAEGRFYVEDEAGQLIPLLLNVQPGQRVLDAC
                     AAPGGKATHLAALMENRGEIVAVDRASTRLDLVMANCRRLGVKIVTPLAGDLRALVGA
                     GIVSHPMLSRPFDRILLDAPCSGLGVLRRHPEGKWYKTPESIAQHRQMQVELLAVTSR
                     LLRPGGVLVYSTCSIEPEETESIIDEFCQAHHQFQRESIAPWLPPAGLPFVTPRGDLS
                     TMANSNRMDLFFAARVRRSE"
     gene            complement(25788..26738)
                     /gene="fmt"
                     /locus_tag="PK224_14370"
     CDS             complement(25788..26738)
                     /gene="fmt"
                     /locus_tag="PK224_14370"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRB16977.1"
                     /translation="MRIVFMGTPDFAVPSLEALLKSEHQVVGVVTQPDRPKGRGQEVV
                     FSPVKVVCQRKGIPVLQPLKMKDPAFLDALRRWTPDVIAVTAYGRILPPAILALPPRG
                     CINVHASLLPKYRGAGPIQWAVIRGEQQTGITTMFMAEGMDTGDMLLQETVEIRADDT
                     AGTLAPRLAEVGGRLLVETLRRLEAGTLTPQPQDDAQATMAPLLKKEDGVVDWTLSAA
                     EIANRVRGLSPWPGAYTHVNGERWVLWRVAVGLETSGAVPGTVTKVSKDRVEVATGDG
                     TIQLIEIQPSNSRRMTMAQYLAGHRLAEGLKLQAIPLSQS"
     gene            26826..26902
                     /locus_tag="PK224_14375"
     tRNA            26826..26902
                     /locus_tag="PK224_14375"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26860..26862,aa:Met,seq:cat)
     gene            complement(26920..28020)
                     /locus_tag="PK224_14380"
     CDS             complement(26920..28020)
                     /locus_tag="PK224_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRB16978.1"
                     /translation="MMALFRRNRVWWMGFSYCGKQVRRSTEVTDKKVAEKIYHKVRTQ
                     IAEGKWYEPARGVDKTVKELLERYLRDHSAVNKSSSSQRSDRSVSVHLIRAFGDLTLK
                     ELRPSLIAAHKAKRRAEGAAAKTINIELGILSHAFQLAVKEWEWVTENPVIRVSREKV
                     RNVIERWLTAEEEQRLLAASPQWLQEIIVFAANTGLRQGEILNLQWSRVDLFRRTITL
                     LEQKNGAKDTLPVNAKTFEVLKARARVRSLKSDYVFFNGAGNRMDARDLLRVFYPVMR
                     KADVKSFRFHDLRHTFATRLVQAGVDIYTVQKLGRWKTISMVMRYAHHHPESLRAGVE
                     ILDRVPAGVSTSLAQSVSHTGAEGGVETAVCG"
     gene            complement(28017..28313)
                     /locus_tag="PK224_14385"
     CDS             complement(28017..28313)
                     /locus_tag="PK224_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB16979.1"
                     /translation="MLTVKELSAWLNIKPSTLYLWVSQNKIPYRRIQGLVRFEPEAIH
                     SWLNSFSSTPSNMPLRVPRQTAEDVDHLIAVAKAAHYTSSREKPDKDRATQGGQ"
     gene            complement(28356..29363)
                     /locus_tag="PK224_14390"
     CDS             complement(28356..29363)
                     /locus_tag="PK224_14390"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB16980.1"
                     /translation="MDSGFTLTIDWLAFTVLASNPQETIRVLGGDWSKAKGGFRGYPL
                     SWMRSDGLRGVGKLGTNAPRRPNEIHVDLSGGLASALTLDQIRTLLQWVHKQQGHVTR
                     IDCALDDRAGTVPVRTIREAVAAGQCVTRAAQVRHIVSNLTHGTGATTGETMYFGSPQ
                     SQTLLRIYDKRLELQSKGQENWPEYGTRWELELKKDRAEQCARALATLDETDWKELVI
                     GLLRSYVDFRQTSKDAEDEERYRAPVLEWYALLTEGFQKGRLAQEQQVQTLQNVKRWV
                     SDTLTPMLAVICATPGGEEWLLNEIVRGIARWNDRHRNLLKQPPNRFHRSAGGHAGSP
                     C"
     gene            complement(31129..31545)
                     /locus_tag="PK224_14395"
     CDS             complement(31129..31545)
                     /locus_tag="PK224_14395"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRB16981.1"
                     /translation="MPQPAVLLDTDILSELLKQHPLVVQRVRNYLAEHQRLAFSIITR
                     YELLRGLKAKQARSQEAAFTLLCQASLILPITDQVVERAATLYGDLHRQGALLPDADL
                     LIAATALDAQRPLITNNLAHFQRIPGLIVESWKRSA"
     gene            complement(31545..31781)
                     /locus_tag="PK224_14400"
     CDS             complement(31545..31781)
                     /locus_tag="PK224_14400"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HRB16982.1"
                     /translation="MRQTIKARYHDGVLQPLEPLALANDAEVQVTVDTDLALGMDEIL
                     RRAAQVYQGLSADEITQVENIALDRQHFFREPAA"
BASE COUNT         6897 a         9953 c         8748 g         6655 t
ORIGIN      
        1 atagagaaaa ttcgtttcac tgggcaaggc ctcgaagccc aaggtcaaca agcctgtccg
       61 gaccttgtcc atttccgcat ggttcaaggc ccgactcgcg cccacatgtg cctcatcgtc
      121 caacgccgcc aacgcggcgc gttgtgccat gctattcgca ttgaagggtg gacggattcg
      181 attcaggtag ttcgtgattt ccggcgtggt catcccatac ccgatcctga gcccggccaa
      241 gccgtagatc ttggagaatg tccgcagcac gatgacattg cgacccgcgt ttacatagct
      301 gagcgactcc gggaattccg gatgccggac atattcgtaa taggcctcgt cgaacaccac
      361 caccacatga tcgggcacgc gcgccatcaa cgccgccacc tcagccttgg tcgccatggt
      421 gccggtggga ttgttcggat tgcacacaaa cagcaggcgc gtcttgtcgg tgatggccgc
      481 tgccatggcc ggcaaatcgt ggtgccaatt cttctgcggc acttccaccg ccacaccgtg
      541 cgcggccttc acctccatct tgtaaatgac gaaggtgtgc tcagccatca cggcctcatc
      601 acccggcgac aaaaaggttc gcgcaagcag gccgaggatt tcgtccgagc cgtttccgag
      661 aatgacgtga tccggcgtga ctttccatcg ttcggccaag gctccgcgca aacgaaacgc
      721 cccgccgtcc gggtaccgat gcagagtcgg agcggtttca cccaagacgg cgagcgcttt
      781 gggcgacggg ccgatcgggt tttcattgga tgccaatttg atcgcgcgcg ggagccctaa
      841 ctctcgctgc agctcttcga taggctttcc aggaacgtac gggacaaggg aggcgatatc
      901 gggatgcacc ttcaatggca tggcgtgact cggacctcta ggaataggcg ggatacgacc
      961 cgagaatctt cataaagaga cagcggctct tcacctcttc ggccgccttc ttgacccgtt
     1021 cttcatcgat atgcccctcg atgtcgacaa agaaaatgta ttcccacgcc ttccggcggg
     1081 aggggcgcga ttcgatcttg gtcatgttga ggccatggga ggcaaacgga cgcaacaagt
     1141 cgtatagcgc acccacctta tctttgaccg acaacatcag cgacgtttta tcgcgcccgg
     1201 ttcgctccgg cgccttctgc gacaacacca gaaaacgcgt gaagttgttg atgttgtctt
     1261 cgatccgggc cgtgatgacc ttcagtccgt acaactggga cgccagttcg gacgcgatgg
     1321 ctgcagccga cggatcgtcg acggacagtt cagccgcccg tgccgtactg gccacttcgg
     1381 acaccggcac gtgaggcaaa ttcgtttcca accaattgcg acattgagca atggcatgcg
     1441 ggtgcgaata gatccgcttg atctctcctg cgaccccgct cttggacatc aggtgatgcg
     1501 ccacttcctg caacacctct ccatagatga gcagactgga atcgacgaac atatccagcg
     1561 tgtgattgac tacgccttcg gtggtgtttt cgatgggcac gacgccaaaa tgggctcgac
     1621 cacgctcgac ttcgctgaac acatctttga tgctgttgac aggaatgtat tgggccgacg
     1681 acccgaactt ctgcatgcag gccatgtgcg taaatgtcgc tcgcgggccg agataggcga
     1741 ccttctgagg cccctcaagg gacaacgagg ccgacatgat ctctcgatac accgcccgga
     1801 tggcatcggt gggaaaggga ccggtatttt gcttgctgag gcgttcaaaa atcgccgcct
     1861 ctcgggccgg cgtgtgcaga tgcgcttccg catctctctg tttcttgagt tttccaattt
     1921 cgatcacgga cttcgagcgc tcgttcaaga ggcgcaaaat ctgatcgtcg atccgatcga
     1981 tttcctggcg atgtccttgc aggtcatcag acatgacggg gggactccct cctcgcgaca
     2041 cctgccttta gagagacgag gccgcgaaac gattccaagc caacgggatg aacttggaca
     2101 agtgagtatc ctacaggagc cggcgagggc attgcaagga taggtgtggg gattcaagga
     2161 gttgtgacgg atgtgtcgtc atcggagagc gacagaaggt ctttctgttt tttgaaatgg
     2221 tcgaaggcca gcctggtcgc ctgccggccg cgaccggtgc gttccagaaa cccggcttgg
     2281 atcagatagg gctcatagac gtcctccagc gtgccccggt cctcctgcac cgccgccgct
     2341 aaagaatcca ccccgaccgg tccgccgttg aacttctcca tgacggtcag gagaatgcgc
     2401 cggtccatct catccaatcc ggcggcatcg actgccaacc agaccaacgc gtcctgcgcc
     2461 acctgcttgg tgatacgtcc accggccttg atctcggcat agtccctgat acgtttaatc
     2521 agccgattca cgatacgcgg tgtgccgcga gcccggcgag caatttccgc agcacctgcc
     2581 tcgtcgatcg gaatattcag cagccctgcc gaccgtgtaa cgatcgacgt aagttcctgt
     2641 gacgaataaa actccagcct gtgaaccaaa ccgaaccggt cccgcaacgg cgacgtcaat
     2701 gctccggccc tggtcgtggc tccgaccagc gtaaaccgcg gcaattccag cttcacggtt
     2761 ctcgtggagg ccccttgacc gaccaccaga tccagttggt agtcctccat ggccgggtac
     2821 agggcctcct cgacagaggc cgggaggcgg tggatttcgt cgatgaacaa cacatcccgt
     2881 tcctgcaaat tcgtcagaat cgcagctaga tccccggcat ggctcaggac taagcccgag
     2941 gtcgaccgaa tcgctgagcc catctcccgc gcaatgatat gcgcaatcgt ggtctttccg
     3001 agccccggcg gcccgtaaaa aatggcatgg tccagcgcct caccacggcg tttggccgcc
     3061 tcgatacaga tctccaggga ttccttcatc cgtgattggc cgacatactc ctgcaagctg
     3121 tgcgggcgca gcgcggcttc aaggccacgt tcgtcgtcgg tcaattgatt gcttaccgtg
     3181 cggtcagaca tgggacgatc ctgttcgaca atgcgtcggc gttatctggc ctcgggtgag
     3241 ggcctgaatt tcggcggcgg cgacacggcc ccttgacctg gcgcagccgt actgccgcaa
     3301 tcaggcgggc acctgactcc accctgaccg ggatcgggca tggttgatcc catgttctgt
     3361 aactggcatt gcgacaccag cccaccatct acagagccgc aacgcgaggg gacgttcgag
     3421 cgccccactt ctacatactc ttcgggacag gtgccgcaga cggagagcat gttgcccccg
     3481 agcggcacgc actgcaccag ggtcggctcg ccgtccttgc acagctccgg cgcatgggtc
     3541 acaccggttg tggcatagcc ctccgggcac attccgcaag tcttgcggat gctcttcggt
     3601 tcctccacac catcggccgc gccgactaac ccggcggatc cggacgagac catcgcggcc
     3661 ataatgacca gcctcaccgt accgactaac cggcgactac cccaccacct gctcaacgac
     3721 ttcatcatgc gtcacccctt ggccaggtct ttgagtgcct cacgaatcac gccttccagt
     3781 ccctgcgcca tagcgccgga cttttcgatc ctcttcaacg cttctttcac atccggttgt
     3841 cgatagccca aattcacaag ggccgacagg gcgtcctcat agagcggatt cgcagatcgt
     3901 ccgctccccg gctcggccgg ttcgcccgct ggatgcaacc gcgcgacctt gtccttcaat
     3961 tccagcgcga tcctcgccgc agactttttc ccgatcccgg acactgtgcc taacttctca
     4021 atatcaccga cctggatggc ggaaaagaga tcgcggactg agagggtgga caggacactt
     4081 aggcccaatt ttggtccgat gccggagatc cccgtcagca acagaaaggc ttctttctcc
     4141 gcggcggtca gaaatccata gagttgaatc gcatcctcac gcacatgggt atggatgctg
     4201 agcgtgacaa gctcatgctg attagggaga gaatagtagg tactgagggg aatcaatact
     4261 tcgtagccga cgccatgcac atcgagcgta acgtgggaag gcgccttgaa ggccatgagg
     4321 cccgtcagta aggcgatcat gtgatcaaca ggggggtgtg atgcgaaggg cccgagaggc
     4381 tggctgcgtg cgcgtcgatg ctcggtgcca atccttccga atcaggcggt agcggcagcc
     4441 accttctcca tgacctcgtc ggatatgtca aagttcgcat agaccttctg cacatcgtcg
     4501 tgctcatcca aaatttccat gagcttcaac atttgctcag cagccttctc ctccagcgcg
     4561 atcgtattct gcgggatgaa gttcaattcg gcaagcgtgc actcgatttt cgcatcgctg
     4621 agcgcttttt tgaccgcctc aaaatcgtgg gtgtcggtga ggacttcaaa ggccttgtcg
     4681 gtcactttca catcttcggc gccggcttcc aaggctaagc ttagcagcgc atcctcttct
     4741 accttcccct tctcaacgac caagacccct tttttatgga actgccaggc caccgcgccc
     4801 gcctccgcca tattgccgcc gttcttggtc aacaaattgc ggatctcggc cacggtgcga
     4861 tttcgattat cggaggtgat ctccatcagc accgccgtcc ctcctggacc gtacccttcg
     4921 agcgtgaact cctcatacgt cacaccgggc aattcacctg tgccgcgctg gatggccttc
     4981 ttcatgttat cgccgggcat gttggcttct ttggccttcg caatggccag ccgcaagcgt
     5041 ggattgccat cggggtcccc gcctgatcgg gcggcaatcg tcaactcgcg gataatacgg
     5101 gtaaagactt tcccccgctt ggcgtcgacc gctgatttat gccgcttgat tgttgcccaa
     5161 tgactgtggc cacccatggc gactcctctt ggcctggacc gaccgtctgt cgatcgagtc
     5221 caacgctcat tgatatgctg attaaaaagg tagagctaac acagtcctca cagaagtgtc
     5281 aacgcgaggc cggccccctg tggccagggg aatgtcctac tctgaataga tgagcaactg
     5341 aagcccaatg aagacaacga ggctcgccca gagcaactcc caatacgaac gttcggtgag
     5401 gacggaccgc gttccccagc cggagaccaa cttggatgat cccgcggtga tcgccatcat
     5461 gcccatgatc acgtgatgga gcacaattgt actggcggca ggatggtcac cgtgcgaatg
     5521 gctgaacaac atgagccctc cgacgatcgc aaacagcgga aggggcaccg tccacaccgc
     5581 atgcgccaac catccggtcc gccgcaggaa ttcaatgaca ccgacggcga aagccagcac
     5641 cccataggtc ttgtgctgga gcacctcctc atcgctgccg aaaaacgttt gcgcgaaggt
     5701 catcgacccg atgggccacg agtcatgatc gctccaaatg agaagaaaaa tacctcccac
     5761 cgtcaaggcg ccgggcaata aaaacctcgt ccaaaccaac gccggccatc ccaaggcctg
     5821 acggacttca ctcagcccga tcagcaacag gaagacaccc gccatgtgat gattgaattc
     5881 cgaataggca atgccctgtc gcgacccctc ccacagttct cccctgacag ccgcatgctg
     5941 atgtccctgc tcctgaacgg ccgcgtcatg cccgtgatcg gcattcccag ccatcacaaa
     6001 tgaaccgctc cacagcaggg cggccagaca cagcatcccc aggaacggaa accatggcat
     6061 caggaatctc ggacaatgaa tcggtcgaga gaaaaacacc ggcaggaatt gaaaggccca
     6121 tccggactgc cggatgggcc gcgcactcgt ctcacgcatg cattcgtcgg ctacataaac
     6181 ttcatgaact tctctttggc ttcatccatc acttgcgccg tgatctcgac caagccgcgc
     6241 tccttggcaa tccgctcaat ttcccggcgg gccatcggac gaatgaagtc aggaatgtta
     6301 tccatgcgtt gttcggcatc cttggtccaa gcaataccgt tgggcgaatc gttcttggaa
     6361 tcgtccatga cctgcagcgt aatgctctta tacccatgct tgcgcgcata ggcttccaca
     6421 ctactctgaa ccattggctt cacaaaagac ggcaagcgct ccaacttctc cttggcgtct
     6481 gcagtccatg tgaattcgct cgttccgttt ccattctgcg gcttgccgcc tgaagttaat
     6541 cccatttccg cgaccatcgc cgagaagggg cacccaccgg acgtcttctc tccaccggca
     6601 tccggcgcgc tgggcttcgc agaagccgca accggttgcc cgccctgaat cttctcgttg
     6661 agataggcgg ccatctgccc tgcgccggcc tgtgcctcct ctttcagcgt cccgcgagtc
     6721 atctcgaacg gttctgccgt ctctgtgcgg cctccgagct tcacaccgag cgaggcaacc
     6781 atttgggtct cgccaggatt cgtcaccatg gagaacttcg cctcgcagga tggacagccg
     6841 aagaagacac caagtgagcc ctcaccgggt ttttcgacct tctgaaacgt catgtaggtc
     6901 tcgcagttca ggcaaacgaa cttcatagga tcccccctcc gggtcggtgt gcttacagct
     6961 tttccgccag gacttttttg taatccagta acgcttgaat ccgcccgacg atttcttgat
     7021 atttctggat cgtcggatac tcaggatcga ggagcggctg ccccttatcg aacgttttgg
     7081 caaacttacg atcgaacggg atacggccaa gcaaaggcag gtccagcacc tcgcacattg
     7141 cctccgtgtt cccctcgaac aactcattct cggcgccgca ggatggacac cggtattcac
     7201 tcatgttttc tacgacacct aacacccgga tacccatgtc ccgcgcgtag gtgacggatt
     7261 tctgcaccac gtcggaagcg acttcggacg gtgtcgtgac gacgatcgca ccggccaggt
     7321 cgggaatgaa tcccgcgatg accggcggtt tatcagcagc ggcacccggc gggagatcgg
     7381 cgaggagata atccaactct ccccagacca catcggccag gaactcgcgg atcacgttca
     7441 tttccatgag gccgagccag accgggctca gatccatcgg gcccttccac cgcaccggcg
     7501 aggcatcatc gaggaaaaaa tccatcgacg ccaccttgat gcctaaggga ccgacgggag
     7561 gctgcgcccc ttccggagtc attcttagcg actgaccgtg taaacccatc atccgtggaa
     7621 cgcaggggcc gttcaaatcg acatcgagca gtccgacctt cgccccctgt cgcgcaaggg
     7681 ccaacgccag gttgacggtc gtcatgctct tgccgactcc gcccttgccg ctcatcacca
     7741 ccagcttctg cttgatgccg gccattcgag cctgcacctg cttcatctgg gcagtgatct
     7801 gctgcaccac tttcgcgtcg tcggaatact gaagcttcgt gaggatcgtc ttgagatcct
     7861 tgccggcagc catggtcatg aaaaaccctc ggaattgttg atgaagaacg ctaccacagg
     7921 ggtttcaata gagtcaaacc gccccgcccc catcgactag atggaatatt ggatggtgga
     7981 cttcgttccg gagggctgga tgagaacccc ccgccgcttc aactcctcca ggatctgacg
     8041 ggcagcggcg tcaaaatcct gggggcccgc gaaatgcaga tcggtattcg gtggcggttc
     8101 ttcccccgcc ctgctcatgt gcacggcaat caccggagcc ggctgaacca acgtccgtat
     8161 catctcggcc attcgctgat aattgatacc gaaggttttg ctggtggaca cgacaatgag
     8221 ccccgtatcg atgagcaacc tggcaacctc tccataccgg cgcacgcgct cggcggcaaa
     8281 ggacggatcg gccgctgaca aatccgcatc caatccctgc agcagatttt cgccttccag
     8341 caggtatgcg tggcggctat cggcgaccaa taacgcttcg acccgtcggg cgagaaacgt
     8401 ctttcccgtc tgggctgaac cggtgaacaa cacgatggcg gcccgatgcc cgtattgcgt
     8461 cgcgcggtcc tctgcccgga catcgcctgt aagccacgca tattcccgct gccgtgcttc
     8521 ctcccgcagc ccttcctgct cgtcatgcac caactcggta atgatcccgc ccccggcaat
     8581 gtcgtactca tcgaccagca cgaaccggcc tgtggcttcg aatgaggacg agagatcgaa
     8641 cgcgagcggc gctttcacac gcagggtcag ttcggcgacc tgattcttcg ccaccgcctg
     8701 gctttcctga agctgcgcga gatcggcggt atcgataatc cgatgaatcg cggccacctc
     8761 gcaggcgact tcacgcgtgg cgaggcgcaa gacgtacttc cgccccttct ccagcggccg
     8821 cctgcccatc caaaacaagt tcacacgaac cgccgtggaa acgagcggaa tggattcccg
     8881 atgggacgcg atttcgccac gctccacgaa aatctgttcg tcgagcgtga tgccgaccga
     8941 ttgaccggcc tgtgcctcac cccgcggggg atcgacattg aatccttcaa tcgattgcac
     9001 gatggctgtc ttgttggacg gcgaaaagac cagttgatca ccgactttca agcggcccgc
     9061 cgtaatgcga ccggccagaa tccgccgcgc atcgaactta tagacgtcct gcagtgggaa
     9121 tcggagcggc tgttccgagc gaacctgctc cttcttgaag agggccagcg tctccagcac
     9181 cgtgggcccc tgataccagt tcatatggct gctgcgcgtc gcgatgttgt cacccatctt
     9241 cgcgctgacc ggaatcatct gttgggggac cgcccggaac tgacccaaaa attcccgata
     9301 ttccttctcg atcccgtcga acacatcctg ccgatacccg accagatcca ttttattgac
     9361 caccaccgca aactgcgtca cacccagcag cgacaggagg tagccatgct tcctggactg
     9421 ctcgcgcacg ccttccaacg cgtcgatgag caacaacgcg gcttccgccc gggccgcgcc
     9481 cgagatcatg ttcttcaaaa actctttatg tcccggtgcg tcgataatga tgtactgccg
     9541 cccgttccag ataaagaacg tgcgcgccgt atcgatcgtg atgccctgct cctgctcttc
     9601 aagaaacgcg tcgaacagga acgcatactc aaattccttg ccctgttggc ggcagatcgc
     9661 ctgcaccttt tccactttgc cttccggcaa ggagcccgta tcggcataga gtcgtcccac
     9721 cagcgtggac ttcccgtgat ccacatggcc cacgatgacg atgttgagac tctcctgcgg
     9781 cttgctctgg tcgtgctgtg tcatatgatt cactcgttca atggaacccc ggttcatgat
     9841 cgtgatcgtt cgaatgcccg ccggctatcc aaccggcgtg ggccgcctca catgtacccc
     9901 ttcttccgga gcatctccat gccccgcccc gcgtcctgcg cgcgcccggc ccgctcggcc
     9961 acattagtcc cgcggagttc cgcgataatc tcatccaccg tctttgccgt ggatttgatc
    10021 ggcatggtac agggcgcgca gcccaaactt cgataccgtg tgccatcccc cctgtccagg
    10081 tatagatcca tgaaagggat gttttcatgc ttgatgtatt cccagatgtt gatctcggtc
    10141 caatcaagga gcggatgaat ccggatatgc gtgcccggcg gaaaggtggt cttgaactga
    10201 tcccacagtt ccggcggctg gtcgcgaaaa tcccaatccc cgtgtttgtc gcgcggggaa
    10261 aaatatcgct ccttcgccct ggtactgtct tcatccgccc gaacaccgag aataatgccg
    10321 gtgtagccct tttcttcgac cagattcttc atggccgtcg tctttaaggc ggtacagcag
    10381 acatcacgcc cgagcgtgtg gttcatcccg gcggccaagg cctctttgtt ctgacccacc
    10441 accagattga ggcgccactc gcgggcaatc cggtcgcgat attcgatcat cgcaggaatc
    10501 ttgtaactcg tatccacgtg gagcaaggga aacggcacat gcccgaaaaa ggccttgcgg
    10561 gccagccaca agagcaccgt cgaatccttc cccatcgacc agagcatggc gaggtgattg
    10621 aaatgcttgt aggcctcccg aaggatatag acgctttgat cttcgagttg acgtaaatgt
    10681 ttcatggttc acactcttcc gtttacgctg aaatgccgac cttcggcgcg tttcccgcgc
    10741 ttgatccggc gaccgccacc aattcagcca gtttggcctt ggcctgaccg gactccaacg
    10801 cgcttctggc ggccccaaga tttccagtaa tcgacgtccc cttgccggct gcatagagca
    10861 acatggccgc attgaggagg acccagtccc gttgcccgcc ctgaatctcg ttggcgagga
    10921 gccgcttgat cagatcggcc tcccgctccc gctgctcgac cgggaacccg gccatttccc
    10981 ggaaggtcgc catcgacaag cccgcatcct tcggctggaa ggtaaatgga gtgatgcgtt
    11041 cgtccttgag ttccaagagc cgcgtggaat tgccgatcga gagttccgga tccccttcga
    11101 ctccgcgaat caccaacgca cggggacaac tcaacatacg cagcgcctcg atcgtctttt
    11161 cgaagtaagg cggatgcgac agcccgatca cttgcgaggt cgcgcgggcg gggttgagca
    11221 tacgggccac aggatgaaag aaattgcgca cgcccaactc ctgccgcatt tccagaaacc
    11281 ggctcaccgg ggggtgatac agcgccaggt ccaggtacgc gaatcccttc ttttccaatt
    11341 cggctccgac cgacttggcg gtcatatcga caggaatgcc caagagcttc agcaccgacg
    11401 agataccgag ccggtccgga ggcccatcca ctccgtgcaa cagcaagacg gctccggcgg
    11461 ccgccgcgac gatggccgca ggaaggatgg cgtgaaaggt ttcccgtttc cccgcataga
    11521 cgggcacatc gacgactccg agcccggaac ggaccggcac cggcggcaca tattggcgcg
    11581 cggtcgcagt gaaggccgct aattccgtga ccgattccga cttgaatcgc atggccgtga
    11641 ggaacgcccc tatttgagca ggggtggcgg ttccctcgat catgaggcgc atggcctgct
    11701 tggcttcttc ccacgtgaga tccttggatg ttttttgacc tttagcgact ttggcgagca
    11761 gatgttgcat ggatggtaat gaccttgtct aatttcaggg tgacgatgat gttacttgtg
    11821 cagcccgcac tcggtcttgc cgaaattctt ccaccgaccg gaacgcggat cgtctcccgg
    11881 aaggacaggc gccgtacaat gggtacagcc gatgctgggg taattgcggt cgtgtaacgt
    11941 gttatagggc acctcatgga gctgcagata cgtccagacg ttttcactgc tccatcgggc
    12001 caggggattc actttgacca ggttgaattt cttgtcccac tcgatcagcc cggcgtttgc
    12061 ccttgcggga gactggtctc gccggatccc ggtgatccag gccgagtact cgccgagaat
    12121 acgagtgagc ggttcgattt ttctgagctc acagcactga tccggctggc tggcccacag
    12181 catggtgccg tgctgggcgg cctgctgttc cggcgtcaac agggacttca tttgaatgac
    12241 ctgtgccggc ttcaacccgt accgcgcgat aatgcgatca cgcacgtcga aggtttcagg
    12301 aaacagaaaa tcggtatcca gataaaaaag cggtgcgtcc ggatcgatac gatgcaccat
    12361 gtccaccagg gcgacatctt ccgccccgaa actgcaggcc agcacaatgc gctgccggta
    12421 ggtcttcaac gcgtactcca gcagttccca tggctgctga gattcaaacg aatcactcag
    12481 tgccttcagc tctgcgtcgg agggtcgtgc agccggcttc tgtgtgtctt tcacccttct
    12541 tacccctcga ccttttccac aagaattttg aaatgcttgg cctcaggctc caacggcccc
    12601 tcatcgagga tcctgtgtcc gtcgtttcgc aagctcatcg gcacattgcg aatgggctct
    12661 ccggcgtcga gccagacttc cagctgctcg ccgttgtcca tcatttccaa tttcaatttc
    12721 gtcttcacat aattcatcgg gcaggccacg ccgcgcagat cgtacacagg cgcctgagcc
    12781 acggcaacgg gtgcaggcga agcaggcacc gcagcaggag ctgcagtagg cacggcttct
    12841 tccttcacct gggccaactt cagatctttg cccatttgtt ccgtggccgc ccgacagaca
    12901 gcgagaaatc gcttggcgaa ggccattttc tccgtcgccg cctcggcagt cgcgtcctta
    12961 gagccaaggt cgccgacttg cgcgccaaga ttcttatagc tggccgggac gatgcccttg
    13021 ctggccagtc gttgatcgaa ctcctggaag gtttcggcat cggtggccgg atcgaggcct
    13081 tcggtcacca aaagaccttt cgcagccgcc agtaccgccc gataggactt attcaccgac
    13141 aaggcatact gatgcttctc gaccagcaat ttcgcctgat agagttcctg atcggcctct
    13201 aacatacggt catcgatcat ctccaaggcg ccacccgcgc actcgccggg gcccagatcc
    13261 tccagcacga attccgcctc accttcccag tcgtagtaat aggtcgagtc ctgctcatac
    13321 ggcggcacga tcgtataggg aatgagttcg tccttgagaa cattcttccc gacacgcgcg
    13381 atgaacaact ggaggctctc accggatttc cgatcccgac gatacacgtc caccagatgg
    13441 cgaactgccg ccggaacgtt cttcgcaggc aacttgactg ccaactgtcc gatcttcggc
    13501 gtgccgtcca catgtccgcc gagatgcagt tcataatgcg gcgccgtatg ttctccgaga
    13561 cgtttgccga ctccgtgcaa cccgatggtg gcgatatggt gctgggcgca ggaattatgg
    13621 cacccgctga tcttcacgct cacatcccga agatcttccg gaacctgacc ggcgggaaac
    13681 acttccgcca gcgccctggc catgccctta gacgaggtaa ttcccaatcc gcaggtatcg
    13741 gtgcccgggc aggcgatgat gtcttcgacg agctctgcgc caggatcgcc caagctgtgc
    13801 tgcaccagct cctgatagaa ctcctccagc cgcccctcgg gaatccaccg gatgaccatg
    13861 ttctggttga tggtcgtccg aatattaccg ttcgaatacc gttcggccaa gtctgcgacg
    13921 acccacattt ggtcgcccgt cagatcgccc atcggcaact tgatcgccgc cgtcacaaaa
    13981 cccgcttgcc gctgtggcac gacattcgtg cgcctccagg cctggaacgc ggagacctgg
    14041 ccattgagtg acacggcacc acccccgcta ccgttgccat gaccgttgga caggacgcct
    14101 gccttggtcg gcataatgag cggaggcgca tcggcatatt cgagtaacgt gatcggctcg
    14161 tgcgtcggca cggcatagcc catggccgcg taggccgctt cccagcgacg cttgaattcg
    14221 tcgaagccga gcttctcgat gacgaacttc atgcgggcct tgttgcgatt cttgcggttc
    14281 ccgagcgtgt cgaacacctt gatcaccgcc tcgatggtcg ggagtaattc gtccatcggc
    14341 gtaaattctc gtaacacctg ggccatgcgc ggcgtcgaac ccaatccacc gcccgccacc
    14401 atccggaaac cgatggttcc gtcggcccgc ttcgccgcga gcaacccgat gtcgtggatg
    14461 ggtgtgagcg cgcaatcctg ccgacatccg gacagcgcaa tcttgaactt gcgcgggaga
    14521 ctctgattca gcggattgcg cagcagatga taggccaccg tcttcgcgta tggcgtgaca
    14581 tcgaacactt cgccttggca cacacccgcc agatgacagg ccgtgacgtt ccgtaccgta
    14641 ttcgcgcagg cctcgcgcgt ggtcaacccc acggaggcca tcagacgcat catctcaggg
    14701 acgtgtttga gctccacaaa gtgcagctga atgtcctgcc gtgtggtgac atgacccacg
    14761 cccgtggcat accggtcggc gagatcggcc acttgccgca attgattccc tgtcagccct
    14821 ccaaacggga tcttaatccg caccatctgg acaccaggtt gccgttggcc gtaaatgcca
    14881 tattgcagcc ggaagggctt gaagatgtcg gtggagaggt ctccgcccag gacacgttgg
    14941 gcttccgcat caaacgtttc gatttcttcg actatatggg ccggaatggg ctccgttttg
    15001 atcgctggac ggagagccac tgtatcagga gtcataataa cgacctcgtc ggttaggaag
    15061 taatgtgcgt gcgcatgatt caggccgctt cagatgtggt acatcaacgt gtgctgcgcg
    15121 tcgagggccc gctggcgctt ggccaactcc tcgaccgtga cttccgaaag aacgctcaat
    15181 tccgcccgtt ttacgcgatc ccaaatgtgg gcgagcagcg cctcctgctt ggcgccacgc
    15241 gaagacgaat gtttcagacc ggcctctcca tttgtcgcga gaagcggccc ctccaatgca
    15301 tcgaggatgt ccgccacgga gagctccgag ggcttacggc tcaacacgta cccgccttga
    15361 gccccgcgct gacttgtcac cactccggct tttttcatcg catgaagcac ctgctcgagg
    15421 aaccgggccg gaatcgcctg tcgcttggcg atcgacttgg cgcaaaccgg ctgttcggca
    15481 tggtggagcg caagatctac ggcggcaata attccgtagg tcgctcgaag gctcaccttc
    15541 atttgcgagt attccgatgg ggattcatgg atctctatac cttgcgcgca actttcctgt
    15601 caagcaaggg tagatgcaca tcaaaccatc atgccgttgg gaaccaagcg ctcatgacgt
    15661 gtctgagaca gccacccgtg cgagcgattc atattgaaga accgtggctc gccagtgcag
    15721 tgccttcagc cgcgatgaac tggtggtagg agccagtcac gaccgtgccc gcgcaccatg
    15781 agttcacccc tgtcgtctgc gcgtcattac aatgcatttt tcgtcattga catcatctag
    15841 gggctgagca ataatcccgc cctcttcttc ctcggtacct tcatgacgct gcgttcatca
    15901 acatttaata ttctcttgct cagcacagac ggcgagatcc aggctcacta caaggacctg
    15961 ttcggcgagc aagcggtcac catcggccgt gacggattgt cgcttccgaa ggaaccggca
    16021 aagcgtgcgt acgatgccgt catcgtggaa tcgaaagcac agtctgctgc agaccttggg
    16081 aaattgtttg gggggatcga tcctgcgcgc acgctcctag tcgtaggctc tcgcaccgtg
    16141 ctgaaacgga cggcaacgtc gctccacgcg gccaaagccc ccaaggcaga cctgtcgcta
    16201 tccaatggaa atgcaccggg gcaatcggtc tgtctggaat cttttctgga acataaagtc
    16261 ggcgatttcg tcaaagggat gcgcaacggc tcgggaagaa acctccatcc gatgctgatc
    16321 gccgccgtcg aacgcccgtt gatcttgctg accctgcggg agaccaaggg gaaccagatt
    16381 caagccgcag agctgctcgg actgaatcga aatacgctga gaaaaaagat tctcgatctg
    16441 gatattccgg tcaaacgcgc cagggcgagc tagattcgcg cgcatctcag ctcttcagca
    16501 cctcaccaga ggcgtagaat ctgtacgccc atatatccac cggtcgccaa ccaccaacgg
    16561 agggaagcaa tgaccaagga agaattgatc gcgaaaatgg cgaacagcgc cggtatcacc
    16621 aaggtcgccg cgacagtggc actcgaagcc ttcaccggcg cagtaaccac gtcgctcaag
    16681 aagggaaaac gcgtgacgct ggtgaacttc gggacgttca cgatttcaaa acgcaaggcc
    16741 aggatgggac gcaatccacg aaccggcgaa gccttgaaga ttcctgccgc ccgcattccc
    16801 aagttttcgg ccggcaaaga actcaaagca gcggtcaagt agacccttgc tgataaacca
    16861 gaggacgcct ggccggtgag acggcttccc actctggaag gctgagagaa aagcctgtct
    16921 tgccgccggg tttccttgac actcttggtg aggctgtatt agcatcgccg ggcgataggc
    16981 gcgtagctca gggggagagc gctaccttga cacggtagag gtcgacggtt caagaccgtc
    17041 cgcgcctacc atttttgttc gcccacacag ttcgtgatcc gactaccggc agcatggtgg
    17101 tcggcacggc gttgtatttt ttgtaggatt gaccggagtg gcctcacagt tcattcacat
    17161 tacccttcct gacggaactc gaaaacaagt accagcagga tgcaccgtcc gagaggccct
    17221 cactccggaa ggtggacgcc tcgacgccaa ggtactggca gccaagatca acggcgagcc
    17281 aatggacttg tcgcgtccgt tagagcagga cgccaccatc gagcctctga ccttcgagtc
    17341 tgccgaaggg cgcgaggtct atcgccacag cagcacccac atcatggcgc aggccgtgaa
    17401 ggaagtgttc ccgacggccc agctgacgat cggtccggca ttggacgatg ggttctatta
    17461 cgacttcgcg ttcgaccgtc cgttcacgcc tgaagatctg gaaaagatcg aggcgcgcgc
    17521 catcgagatt atgaaacgtg gtctgacggt tacccgcagc gagctgtcca agcaggacgc
    17581 catcaaattt ttccaggaac gcggggaagg ctacaaagtc gagctaatca acagcttcga
    17641 cgatgcctcg cctatttcgc tctaccgtca gggggaattc gtcgacctct gccgcgggcc
    17701 gcacctcccc acgaccgggt atgtgggtgc cttcaagctg ctctcgaccg gaggcgccta
    17761 ttggcgtggc gacgagcgga acccgatgct gcagcgggtc tacggcacgt ccttcccgac
    17821 caaaaaagaa ctcgatgccc atctcgcgaa gctagaagag atcaagcgcc gcgaccatcg
    17881 gaaactcggc aaggagctcg acctgattac gattcaggac gagatcggtc ccggcttggt
    17941 tctgtggcac cccaagggct cgctgatccg gctgctcatc gaaaacttct ggcgggagca
    18001 acatatcaag gacggctacg acctggtgta ttcacctcat gtcgcccggc tggacctgtg
    18061 gaagaccagc ggccacgtcg attactaccg cgaaaacatg ttcgcctcga tgaagctgga
    18121 aggcagcgag tatcaactca agccgatgaa ctgccccttc catatcatga tctacaaatc
    18181 ccacatgcgg agttatcggg atctgccgat ccgttatggc gaattaggta ccgtctaccg
    18241 ttacgaacgc acgggtgtgc tgcacggcct gctgcgtgtc cgcgggttta cccaggacga
    18301 cgcgcatctg ttttgccggc ccgatcagat cgaggccgaa gtcagccgcg tactggactt
    18361 cacatttttc gtcctcggta ccttcggctt tacagagttt gaagtgtatc tttcaacccg
    18421 cccggagaaa tccgtcggct ccgaagaaaa ctggaccatc gccaccaatg cgctggaagc
    18481 tgcgctcaag agccgcggtg tggcctacga agtggatccc ggcgaagggg tcttctatgg
    18541 cccgaagatc gatatcaaaa ttaaagacgt cctcggccgc tcctggcaat gctcgacggt
    18601 ccaggtcgac ttcaacaatc cggagcggtt caagctggcg tacaccggcg aagacggcaa
    18661 ggcgcatcag ccgatcatga ttcatcgggc gctcatgggc tccatcgaac gcttcttcgg
    18721 catcctgatc gagcactatg cgggcgcctt cccgacctgg ctggcccccg tacaggcggt
    18781 ggtcctcacg atcacggaca accagcaaga atttgcagcg aagatcgtct ccacgctcaa
    18841 gagccacggc tttcgagttg aggcggacat ccgcaacgag aagatcggct ttaagatccg
    18901 cgaggcggaa aagaacaaga ttccttatat gctggtcgtg ggagacaagg aagtgcagag
    18961 cggcatggtg tcggttcgcg gccggagtgg cgcgaatcac gggagcctgc cgattgaagc
    19021 gttcctggaa ctccttcgca cagatgcgaa tcaaacattg cgtcaaacgg caacccactc
    19081 acaaacgagg tgacctatcg tccctaaatt acgcgtaaac cgggaaatca ggattcggga
    19141 agttcgagtc atcggtcctg aaggcgagca actaggaata ctgccgaccg tagaggcctt
    19201 caacaaggcc caggaggggg gctacgacct tgttgaggtg gctccgacct cgcagccgcc
    19261 ggtttgccgc atcatggact atgggaagta caaattcgaa ctcagcaaga aagatcacca
    19321 gagccggcgt caccagaagt ctacgcaggt caaagagatt aagcttcgtc cccgcaccga
    19381 caagcatgac ctcgagatca agatccgcca gatcaaggaa ttcctggccg acggcaacaa
    19441 gaccaaagtg acgttgacct atcgcggacg cgaaatggcc aatcaggaga tgggccgaac
    19501 aatgatggcc agcgtcatta aagaatgtac ggaagcaggg accgtggagt ttgcgccacg
    19561 aatggaaggg cggagcctga tcatgatttt ggctcccaaa tagtgtgccg agtgcacaca
    19621 aggaaggaat gagagcatga aaataaaaat gaaaacccac agtggcgcga agaagcggtt
    19681 tcgacgcaca ggaacgggga agctggttcg ccggaaggcc ggcggtcggc atttgctcac
    19741 cgggaagcca cgcgaccgca agcggaacct gaagggggcc gttgaggtgt cctccgcctc
    19801 gaccccagca ctgaaccgca tcattccgca gtaacgacga ttgtgccgtg aacaggatct
    19861 gaaaaaggag tagctcccga tgcctcgtac aaaaggtggt ccaaaaacac gacagcggcg
    19921 aaagaagcgc ctgaaactgg cgaaaggcca gtatggcgcc aagagccggc tgttccgaac
    19981 agcaaccgaa tcggtcgaca aggggcaggc ctatgcctac gtcggacgaa agaatcgcaa
    20041 gcgcgatttc cgccagctct ggatcgcacg catcagcgct gcgacccgcc tgcatggcat
    20101 cgcgtatagc cgtttcatga acgccctgaa gaaggcgaat atcctgctgg atcggaaagt
    20161 gttgtccgat atggcgatcc gggatatggc gggatttgag aaactcgtcg gggtggccaa
    20221 gcagcaactc gcaactgtcg ctagctaacg gttacgtccg cacgaacgtt ctgtggcggg
    20281 agaatcctga tcgaacggat tttcccgtca cacctcttcc agtagtcgat cgatctccac
    20341 ctccagcgcc accgccggga ccccgatttg ccagtgctcc agcacctcgg gatgcagctc
    20401 gcccaacaac ccgatgaccc gaccattcga tacgatcttc cccgctcgac catcgagaaa
    20461 tgagggatga tccaccggct ccagggtaaa ggggcgatcc aggtaataca gcagcagatc
    20521 cagacaggaa tgaatttccg agaagtgcgc gccggcatgg gcaatcacgg cacccaacat
    20581 cgtcacggtc cgggatccga catcggcact cgcatcagga atagccactt ccccgacctc
    20641 aaacatgcgg tgcggataga aggcccggct tgaattggtt tccacctgca acaacgatgg
    20701 cgtaatccac tggcgcaggc aggagtagct gagagacatc acgttatcca cttccaccac
    20761 cttcgcccac tcggtgccat ccagacgcat gcgtgtgcaa aagtcctgat gagagcccat
    20821 gatgttggaa atgatttcct ggaattccag acccaccatg agatgccgaa cccggtcggc
    20881 catctgctcc aaacgcgaca gcccacccac cgtaaactgc gagggcatga cgggggcaaa
    20941 cctggcgtat ccctgactga tggccacatc ctccaccaca tcgaccgtat gcagcagatc
    21001 gttgcggtag ggcggtagtt gcacagccac cttttgcccg gccgacttta cggtgtaccc
    21061 ataggacttg agcgccgagg cgacttcctt gccacccagg gcctctccca acgccgactc
    21121 gatggccttg agaggaattg tccgggattc cccgaaatcg atcggcgtac tccagcgtcg
    21181 cccgaagttg gttttcaccg gcgagtgaat ttcgaccggc gtgatcaccg cgccgcgatc
    21241 cgcgagattg gcggcaaaaa tattcagggt cagcgcgacc atcgaaagat cggtcccggt
    21301 cacctcgacg aacaattcat gatccccgac ctggacctcg ccgatttccc ggctgttgat
    21361 gatcggggga aaggacaaca cctgtccctt ggcgtcccgc agcacaggga gccggtcgtg
    21421 gccaccgaca atgccgccat actccacgcc tttgggatga accatgagga tctcccgtag
    21481 cgtcatcatg gtttccatgc cgagcggcgt gaatcgcacg tcgtccggct tgaccagatc
    21541 gtaggagacg gggaacacga tgggagccaa acggtataag ccgatcgaga ccgaccgccg
    21601 cttacgccca aaaatatcgg ctaacttttc ctgcgtttgg atgagctgcg tcaggccgga
    21661 tgccgtcacg cgatacccga ccgccgtgca agcggccacg tacggacgaa tctggtccat
    21721 tccctgcgcc acaaccagct tccccgccac tcccttcggc ttcttgaaga agggatagga
    21781 aatcgccgag ccacgctgct tgatccggat ctgcctggcg atgccttcac aacaccacaa
    21841 gtccggacga ttgctgtctt gcaactcgat ccgcagctcg ccggtctctg aattgtgccc
    21901 cttcagctct cccttcacca acatcagcca ttgctcaagc tgatcgagcg gaatccgcgc
    21961 cggctcttcg tccggtccgg caattaaggc ttcgagatcg tctcgttgaa gggaaatcgt
    22021 gggcatcgtg tcgtcgtcct aaaaacttcc ccgcatggtg cgaatgaatt cgaggtcggc
    22081 cgagaacaaa tcgcgaatgt cgtgaatgcc cagcgccacc atcgccatac gatcgaggcc
    22141 aagaccccag gcaatgaccg gcacagagac accgagtggg gtcgtcactt cgggccggaa
    22201 taatccggcg ccgcccaatt ccatccaccc cagcttcgga tggcgcacat gcatttcgac
    22261 cgacggctcg gtgaacggaa aataggccgg tagaaatttg acttccttgg cttgcgccac
    22321 ttcccgggcg aacagattca acaatccgag cagggtccgg aaattgatat ccgcccctaa
    22381 cacgatgcct tccacctgaa agaagtcggt ggcatgggtc gcgtccacct ggtcgtaacg
    22441 aaagcagcgg gcaatcgaaa aatatttccc cggcacggaa gcgccggctg ccaatgtcct
    22501 cgccgacacc gcggtgcctt gactccgcaa caccagccgt ttcgcccgtt cggcatcgaa
    22561 ggcatagccc caccccgtcg atccggcccc cgtccctttt tcatgcacct gcgtcacctg
    22621 cgtcaggtac ggctcggcga ttgtgcgggc atgggtcgga tttttcacga aatagacatc
    22681 atgaatgtca cgggccggat gaaactgcgg catgaacagc gcgtccatat tccagaactc
    22741 ggtctcaacg agcgtcccgc gcatttcctg gaaacccata ctgaccagct tgagtttgac
    22801 gagatccaga aactcgcgat agggatggcg tttcccggcc gcgattctcg gggcccgcaa
    22861 actgatcgta tatttccgaa accgcttcgt ccgccaggca ccttccttca gcaactccgg
    22921 cgtcagctgc gagacttcct ccgccacacc atcccgcgac agctgctccg ccacctcctg
    22981 cccggcaggg gtcagcacaa atgaccgggt tacccgctca tcgatccgga acggctcacg
    23041 cgcattccca cgcttcaccg catgttgctg gagcacgctc cgaagtggtt cggaaaaaga
    23101 ctgcaactcc cgctggccgc tccgcaattc ctgcaacaac gagcgcatgg cctcggccgt
    23161 ggcactattg cggccggtac tttcaatgca cccgccctgg acaatcagga tcgcgccctc
    23221 tttcttgagg gtgccgaccg ccttgctcac atcggacggc tcgagtgctt ccttagcctg
    23281 aatatcctga atcgtgagac gtttccccgt ctggccggct tcccgcgccg cagagagcac
    23341 tcgttcaatc ggggcatatt tctcaaaaaa caattcgccg atcggggtca gagatgcgat
    23401 atgcgcgacg gtttcagcct gcacggcgat cagcgatttg gcgagcagcc attcgacggc
    23461 catgctcaac tgcgacggct ccagcccggt tgatgccgca agttgatcga gcgtaggagg
    23521 cgcgtccggc tgccgggtca acgccagcag gactttgctt tcgaggggat gaagattatc
    23581 catgaacaag ggtgtggtcc gacgcctctt ctatcgctga gcggttgcaa ccctcgcggt
    23641 gcgagcggca ggctcatgtc cggctctgag cgccgtaatg gcagcggcat agtcatccgt
    23701 ggaaaatacg gccgaccccg caaccagcac ctcggcgccg gcggcgagaa tctcttgtac
    23761 gttgtccggc ttcacgcctc cgtctacttc cagaagcgcc cggctcccgg tgcggtcgat
    23821 cagagccctg acctcggcaa tcttgcgtaa cgacgaggca ataaacttct gcccgccgaa
    23881 cccgggattc acggacataa tgaggatgag atcggcatca cggacgattt ccgaaagggt
    23941 accggccggc gtggcaggat tcaacgtcac acctgccttc acgccccgct ctttgatcga
    24001 ctgcactgtc cgatgcaggt gcgggcaggt ttccacatgt acggtcagat agtcggcacc
    24061 cgcttcggca aactccccga tgaacgcatc cggattcgtc atcatcagat gcacatccaa
    24121 gggcagggtg gtgacttttc tcaaggcttc gacgatcggc ggtccgaccg tcaggttcgg
    24181 cacgaagtgc ccgtccatga catcgatgtg caaccagtcg gctccggcgt cctccacccg
    24241 cgccacttct tcggccaggc gcgcgaagtc cgccgacaaa atggaggggg caatacgtac
    24301 cgtccgaccg gccattactc gctcctccgc acacgggcgg cgaaaaacag atccatcctg
    24361 ttactattag ccatcgtaga cagatcccct cgaggggtca cgaacggcag tccggccggg
    24421 ggcaaccagg gcgcgatcga ctcacgctga aattgatgat gagcctgaca aaactcgtcg
    24481 atgatcgact cggtttcttc cggttcgatc gagcaggtac tatagaccaa cacccctcca
    24541 ggccgcaaga gacggctcgt caccgcaagc aattccacct gcatctgccg atgctgcgca
    24601 atggactccg gcgtcttata ccacttccct tccggatgac gccgcaacac accgaggccg
    24661 ctgcacggag catccagcag aatgcggtcg aacggtcggg acaacatcgg atgggacacg
    24721 atccctgcac cgaccagcgc gcgcaaatcg ccggccaggg gcgtcacgat cttcactccg
    24781 agacggcggc aattggccat cacaagatcg agtcgcgtcg aggcccgatc caccgccacg
    24841 atctctcccc gattctccat gagcgccgcc aggtgcgtgg cctttccacc cggcgccgca
    24901 caggcgtcca gcacccgttg ccccggctgc acattcagca acaacggaat cagctgcccg
    24961 gcttcatcct ccacatagaa ccgtccctcc gcgtatcccg gcagatcgat aacggaacag
    25021 gagcgagcca gttgaatgcc gacttcgctg atcgacgtgg ggccggcctc cacctgcgcc
    25081 gcagccaagt cggccgacaa tgccgcgcgg gatgttcgaa gcgtattcgc acgcagcgtc
    25141 agcggaggca cctctaccga cgcacgacac aagacctcag cgcgctcggc acccaagcga
    25201 tgacaccaac gttccacgag ccaggccgga caggaatacc gcacagccaa ggccacaacg
    25261 ggatcgcttc ccgcatcggg ccaggccggt tcgggtgatc gaagcagagc ccttagcacc
    25321 gcattcacaa agccgctcca gtcccgaccc agacgacggc tctgctgctt cgtcatctgc
    25381 acagactcgt tcacggcagc ggaatccgga acccggtcca agtacaggag ttgataggcg
    25441 ccgagacgca gaatggtctg aaccagggtg ggaagtttcg taatacgccg atccgacaac
    25501 agacctaagc gccagtcgag agtggtccga taccgcaaca ccccgcgcac cagctcgacc
    25561 atgaaggccc gatcgcgcag atccaacgcc tcgcgtgtgg acacctgatc gaacagatcg
    25621 tctcccaaca tgcccgctgt gtcgatcgcc agcaacgcct tcatggcggc gcgtcgcccg
    25681 gcagatacaa tcccgcgtga ttttgacggg ggaagatcag aggccatgtt cagaaaggat
    25741 gatcatggcg agaagatagt cgatagaatg cgatgatggg cgcgcgatta gctttgcgat
    25801 agagggatag cttgcagctt gaggccctcg gccagtcgat ggccggccag atactgcgcc
    25861 atcgtcatcc gcctgctatt ggatggttga atctcgatga gctggatcgt cccgtcgccg
    25921 gtcgccacct caacccgatc cttgctcacc ttcgtcacgg ttccgggaac cgcgccggag
    25981 gtctccagcc ccaccgccac gcgccagagc acccaccgtt ccccgttcac atgggtatac
    26041 gcgccgggcc atggagacag gccgcgcaca cgattcgcaa tctccgcggc cgacaaggtc
    26101 caatcgacca caccgtcttc cttcttgagc aatggcgcca tggtcgcctg tgcgtcatcc
    26161 tgcggttgcg gcgtcagcgt gccggcttca aggcggcgca gggtttcaac caacagtcgc
    26221 ccgccgacct cggccaaccg aggggccagc gtgccggccg tatcgtcggc acggatctcc
    26281 accgtttcct gtaacaacat gtcgccggta tccatccctt cagccataaa catggtcgta
    26341 atgccggttt gctgttcccc cctgatgacg gcccattgaa taggccctgc cccacggtac
    26401 ttcgggagga gcgacgcatg tacgttgatg cagccgcgag gcggcaacgc cagaattgcc
    26461 gggggaagaa tgcgtccata ggccgtgacg gcaatgacat ccggcgtcca ccgtcgcaag
    26521 gcgtcgagaa aggcgggatc tttcatcttg aggggctgga gcaccggaat ccctttccgc
    26581 tggcagacaa ctttcacggg cgagaagacc acttcctgcc cgcgaccctt cggccgatca
    26641 ggctgagtga ccacaccgac cacctgatgt tccgacttaa gcaaggcctc cagagaaggc
    26701 acggcaaagt caggagtccc cataaatacg atacgcatgc tcacgcttta tcattctgcc
    26761 ctcgcgaatg caattctccc cggacgttcc ttgactctcg tttcacacgc ctgttagtat
    26821 ccggtcgcgg ggtggagcag cctggtagct cgttgggctc ataacccaaa ggtcgtcggt
    26881 tcaaatccgg cccccgcaac caaccttctc tcttcacttt caaccacata cagcagtttc
    26941 gacaccgccc tccgcacccg tgtgagaaac cgattgtgct aaacttgtgc taactcctgc
    27001 cggaacgcga tccaagatct ccacaccagc acgcaaactc tcaggatgat gatgggcata
    27061 ccgcatcacc atggaaatgg ttttccatcg tccgagtttc tgcacggtgt aaatatcgac
    27121 tccggcctgc acaagacggg tggcgaaggt atggcgcaga tcgtggaaac gaaagctttt
    27181 cacgtctgcc ttgcgcatga caggatagaa cacgcgaagc agatctcgcg catccatccg
    27241 atttcccgca ccgttgaaga acacgtaatc gcttttgagc gaacgaaccc tggctctagc
    27301 tttcagtacc tcaaacgtct ttgcattaac aggaagggta tccttcgctc cgttcttctg
    27361 ctccagcaac gtaatggtcc gccggaagag atcaaccctg gaccattgca ggttgaggat
    27421 ttccccctgc cgaagccccg tatttgcggc aaagacaatg atctcctgaa gccattgcgg
    27481 tgaggccgcc aacaatcgct gctcctcctc cgctgtaagc caccgctcaa tcacgttacg
    27541 cactttctcc ctcgatacgc ggatcaccgg attttccgtc acccactccc attctttgac
    27601 ggcaagctga aaggcatggc tcaagatccc taactcgata ttaatggtct tggccgccgc
    27661 cccttctgct cgtcgcttcg ccttatgtgc tgcaataaga gaagggcgca gttccttgag
    27721 ggtcagatcc ccgaatgctc gaatgagatg caccgacaca gacctatcac tgcgctgaga
    27781 ggaagaagac ttattgaccg ctgagtgatc tcggagatag cgctccaaca attccttcac
    27841 tgtcttatcg actccccttg ctggctcgta ccacttccct tcagcgatct gagtcctcac
    27901 cttgtgataa atcttttcag ccactttctt atccgtcact tcagtggatc ttcgaacctg
    27961 tttgccacag taagaaaacc ccatccacca gactcggttt ctccgaaaca gtgccatcat
    28021 tgacctcctt gtgtggccct gtctttgtct ggtttctcgc gactggaagt atagtgagcg
    28081 gcctttgcga cagcaatcag gtggtctacg tcctcagctg tctgccgagg cacacgaagg
    28141 ggcatgttcg aaggcgtgga agaaaagctg ttcagccagg agtgaatcgc ctcaggttca
    28201 aagcgaacga ggccttgaat gcgacggtaa ggaattttgt tctgtgagac ccagagatac
    28261 agtgtggacg gcttgatgtt gagccaagcc gaaagctctt tgacggtgag catatgcaga
    28321 tacaccgagg gagtcttgcg acacccccaa tccccctaac atgggctgcc cgcgtgaccg
    28381 ccggcagacc ggtgaaaccg attgggtggt tgtttgagca aattgcggtg tcggtcgttc
    28441 caccgagcaa tgcccctgac aatttcgttc agtagccatt cttcccctcc aggggtggcg
    28501 caaatcaccg ccaacatcgg cgtcagggtg tcactcaccc atcgtttcac gttctgcagg
    28561 gtctgcacct gctgctcctg ggccaatcgc cccttctgaa atccctccgt caggagggcg
    28621 taccactcca gcactggagc ccggtagcgt tcttcatcct cggcatcctt tgaagtctgc
    28681 cggaaatcca cataggaccg gagcaaccca atgactaact ccttccagtc ggtttcgtct
    28741 aacgttgcca gtgcccttgc gcactgttcg gcgcgatcct ttttgagctc taattcccac
    28801 cgagtcccgt actccggcca gttctcttgt cctttgctct gaagttccag ccgtttgtcg
    28861 taaatgcgca ataaggtctg actctgagga ctcccgaaat acatcgtctc acccgtcgtc
    28921 gctccggtcc catgcgtcag gtttgagacg atatgccgga cttgagccgc cctcgtgacg
    28981 cactggcctg cagcaaccgc ctctcgaatc gtcctgaccg ggaccgttcc cgcccggtca
    29041 tccagggcgc aatcaatgcg cgtgacgtgt ccttgctgct tatgcaccca ctggaggagg
    29101 gttcggattt gatccagtgt cagggcggac gccaggccgc ccgacagatc cacatggatt
    29161 tcattcggac gacgaggagc attggtgccc agtttgccga ccccgcgcag accgtcgctt
    29221 ctcatccagg acaaaggata ccctcggaac ccacccttcg ccttgctcca atctccaccc
    29281 agtacccgta tagtttcctg gggattactg gccaggaccg taaaggccaa ccaatcaatc
    29341 gtgagcgtga agccagaatc catggaacct atcgaactcc tggacggagc gctgtgggta
    29401 gcgcccccgt gttaccaaga cgggggccat tccggtccgc cccgcagcct ctcggcatgc
    29461 ggcgcggacc ggctttgtct cccggccagg gacgacggtg ttgctgtgtg acgtgctccg
    29521 attcgtacat ccttccctcc ggttgagccc tgttcccgtt cgtcgcaccc taggcgaatc
    29581 accggaccga agcaaaggcc ccggaagggg acgctagggg tcacgagggg acaggagagg
    29641 acagattttg ggacagccaa ggcacgaaag gaggtaggca taaagatgct taagtgcgcg
    29701 ttgctgcgct ggtggcggca attgtgtgta tcgtcctgaa ccggaacatg gaggaaacag
    29761 aaatagactc gtttttggct ggtgcgagaa aaaaaagttt ggaaggcatt accggcgttg
    29821 tgggatagga tcagatggag gctaataaga taaagcgttc ggggaacgat aaggggagag
    29881 tgtggtctcg cctagattcg cgccaccaac caatacacga ttgccagaac attctttaat
    29941 tcaattccag gcactatgag gcgactcacc acaatgattc aatacgctag ctggggagca
    30001 ctcgcaggta tcatcccgcc cattctaatt atggcgatga cgtacgtcca aggcggagtg
    30061 tttcagtggc caacattagc agtggctctt tggcccacgt cactaatgct tatgggaaca
    30121 atggggcaag agctaaccgc ttatggaatt ataatttttg tcctttccgt attgtcgaat
    30181 caaattgtat atgccgtgat gggaatcgtg gcctggctct tgtcgcatcg ctcatagagg
    30241 agacatgaca agaggccgcc atatctgagt cagatttcta tgagtgcaca agagattgtt
    30301 ggtcttgtca ggtgtcctat acaagatgat cggccgttct cctaagagag ataaagaata
    30361 agccacccga ccaagaatag gagccaattc gtgcgccgaa ctattctttg gtgggccatt
    30421 gttggtatag cggttccaat gatgatcttg ctagttggcc atttacaggg gggcgttttt
    30481 gcttggccac gtgctgcatt agtgatgtgg ccgacttgga ttcttaatgc ggcgacgttt
    30541 ggaagagagt ccagtatctt cggtgcctcg gtgctaatta tttcgatctc taccaatggt
    30601 ctcctttacg cactggttgg tctggtgctt tggggattgt taccgaaaca ttttcgtcct
    30661 actaggtagt cggagaagtg attcaattgc gagcatccct gaagaccacc cgggacgtca
    30721 ctacatcggc aatttcggcc ctgacgcgtt tgcgtgacgg gctctagagc agacgcgcca
    30781 ctcgcctacg taacaacttt tcaccttcaa gaacggatga cattcttgcc catcatcttt
    30841 gccctattct tcttttccct tggagccctg cttgtgtacg gcacctacag gagatggcct
    30901 gttttagtag atcctcctga taaatgggca ccattttact ctcaggcttt actgaaaaag
    30961 tattttggtc gcacctttct gatcgcttat aactacatcc tcggtctgct gtttattgta
    31021 ttcacgtgca taggactctg gaacggattt acgaaatgac tccacccatc ccggcaggtc
    31081 ttgtgaggtc gacgaccaga ttgtctagaa gcatccgtct aactggggtt aggctgaccg
    31141 tttccagctt tcgacaataa ggccgggaat gcgctggaag tgcgcgaggt tgttcgtaat
    31201 caacggacgc tgcgcgtcca aggcagttgc ggcaatgagc agatcggcat cgggcagcaa
    31261 ggccccttgt cggtggaggt cgccgtataa cgtcgccgcg cgctctacaa cttggtcggt
    31321 gatcggaaga atgaggctgg cctggcagag caacgtgaac gccgcctctt gactccgtgc
    31381 ctgtttcgcc ttcaagcccc gcaggagttc gtagcgcgtg atgatcgaga aggccagccg
    31441 ctggtgctct gccagatagt tcctcacacg ttgcacgacg aggggatgtt gtttgagaag
    31501 ttcggagaga atatcggtat cgaggaggac agccggttgc ggcattaggc ggccggctca
    31561 cggaagaagt gctgccgatc taatgcgatg ttctcgacct gcgtgatctc gtcggcactc
    31621 agcccttgat agacctgtgc cgcacgccgc agaatttcgt ccataccgag cgcaaggtcg
    31681 gtatcgaccg tgacctgcac ttccgcgtca ttggccaagg caagaggttc caacggctgt
    31741 aacactccgt catgataacg ggctttaatt gtttgtcgca tcgtggagcc ttcctccgca
    31801 cgccctctga atgagttagc cagccgctcc cattctactc aaaccggaac caaatctcat
    31861 cacttgtcga aagatcgtcc ctcagcgtgc aaaataaacc aggtagtcgt ggttcctcac
    31921 aagctgtctc agtgaagggg ttcgccccat atcgctgtgc taaatttgtg ctaaacctca
    31981 gcgaaaagaa acagaaacac tagacgaaac tgcatcaaat agaacgccgt aaactgctga
    32041 ttcagttaga taaagcgaga aaccagtcga aagatcagag cgttagcaaa ttacatatta
    32101 ggcatctcat aacccaaagg tcgtcggttc aaatccggcc cccgcaacca accttctctc
    32161 ttcactttca accacataca gcagtttcag tccagacatg cgtattccga cccaagccgg
    32221 ccgctgattc cgacgcaagt cggccaccca ttc
//
LOCUS       DAORRQ010000047        32166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_83_length_32166_cov_27.943633, whole genome shotgun sequence.
ACCESSION   DAORRQ010000047 DAORRQ010000000
VERSION     DAORRQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 32166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32166
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_32166_cov_27.943633"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1376
                     /locus_tag="PK224_14405"
     CDS             159..1376
                     /locus_tag="PK224_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRB16983.1"
                     /translation="MTTDSQYQDFVPPHRLLMGPGPSMVHPRVLQALSQPLLGHLDPV
                     FLTLMNDIQALLRATFHTQNEFTIALSGTGSAGMEAVIANLIEPGDRAIVGVNGVFGT
                     RLATMIERSGGLPIRIEAPWGATIPIEALEQELARSAPVKALVLVHAETSTGALQPLE
                     DVGTLCRAHDALLIVDAVTSLGGIPVEVDTWGIDACYSGTQKCLSCPPGLAPLTVSPR
                     GMGAIRQRRAPCHSWYLDLSLIADYWNDHSRAYHHTAPISMLYGLREALRLVHEEGLP
                     ARAARHQVNSDALMAGLQTLGLNPLPPPGHRLPMLNCVTLPDGIDEATMRTQLLQQYG
                     IEIGGGLGPLRGRVWRIGLMGESCRQAHVLTLLNALEEIFAGQGRPAQPGRAVRAAAE
                     TYTHSLGSARRGA"
     gene            1373..1975
                     /locus_tag="PK224_14410"
     CDS             1373..1975
                     /locus_tag="PK224_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB16984.1"
                     /translation="MSRNGLWIVAAGVAGFVAAITYWIVALAVAESHKAEMVPPERVT
                     SFIHAVIDANRANYTQNVVDKLHNQGVVEALEHWKEEKGLPLPAQFLLESGRLVAQKD
                     MKLSFRLASLTPIYVWNGPNSEFERRGLEVVMKAPEKPFTGFYQQAGVRYFQGIYADR
                     AVSESCVSCHNAHVNSPRRDYKLNDIMGGVIVTIPISEAP"
     gene            1972..3180
                     /locus_tag="PK224_14415"
     CDS             1972..3180
                     /locus_tag="PK224_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRB16985.1"
                     /translation="MTIAIRHIRILDGLGHRIERGTLLIEGTRIQAVGPEQDVRLPKG
                     AQRIDGRGLTVLPGLIDCHVHFCLGAEADVVAAVEGEPSSLTLLKAAELARRTLHAGF
                     TTVRDVGFRDHAVFALKQAIESALTPGPRILAAGLAICMPGGHARFIGREADGVEAVR
                     AAVLDQLNAGAEVIKVIASGGVLTPGTSPDDAQMTVAELSAAVEVAVAHGRHVAAHAH
                     GATGMKNALRAGVHSIEHATLMDDDAAEMMTQRHVYMVPTLSALATTAACPTGCGIPD
                     SARSKARNMVKQHERSFRAALRRGVPIAMGTDAGTPFNYHGDNAQELERMVALGMTPM
                     EAIMTSTSAAARLLGLIQDIGSIAAGKQADLLVVEGNPLRNIGVLLKRERIAGVMQRG
                     RFVAGPLSRA"
     gene            complement(3206..3844)
                     /locus_tag="PK224_14420"
     CDS             complement(3206..3844)
                     /locus_tag="PK224_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRB16986.1"
                     /translation="MPRLDYYRVLGVSRDISDDDIKKAYRKLVFEHHPDRNPHNKQAE
                     EKIREINSAYEVLGDPESRRTYDRLHWGEEARAETVDPSLILEEMEKKLFDEGRKEVF
                     AVLMKMVPRIKSELALIRERTVAHQGYDTFKESIVEARGAEVLDEFVTSDMEQRKQRL
                     LEVAVQMMVSQAVVAKGDEGGIRALRGRLEEMFRKGRINGFATALELLYERR"
     gene            3953..5233
                     /locus_tag="PK224_14425"
     CDS             3953..5233
                     /locus_tag="PK224_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB16987.1"
                     /translation="MAWAQGVTRYQWLVLFVAWLGWVFDAMDATIYAIVLHPALHELL
                     QAGAASGAVTNERIGWYGGMVFSIFLIGWAIGGIGFGMAADYFGRTKTLIITILIYAV
                     FTGLAAFAQEWWHLAIYRFLTALGIGGEWAAGAAIVAETWPEDKRARAAGILQSAWAA
                     GFFLAAGFNLLLSDYGWRLLFLVGILPAFVALLVRWWVKEPERWVQAHEQEAQSVRNS
                     FMHLAELFQPALRRNTWVGSTLAFVAVFGLWGATNWAPTLIRAMPDLQGQDAATLTQK
                     VSYAIMALNAGSLFGYLGFGPLAERFGRLPVFGLMCLGSFIMLPATYFVPHSYAEVLL
                     LLPLLGFFNNGIFSGFPIYLPELYPTRLRATGSGFCFNAGRVLASAAPFVTGWLVTLL
                     GSFNNAASTIALIYLLGLAVLPFATETNGKPLPE"
     gene            5262..5966
                     /gene="pxpB"
                     /locus_tag="PK224_14430"
     CDS             5262..5966
                     /gene="pxpB"
                     /locus_tag="PK224_14430"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRB16988.1"
                     /translation="MKQPETALPRIVPLSESALTIEFGETIDPHTNESVLAFATAVER
                     AGLPGILEVVPTYRSVTVYFDPFRTDVATMTGHFRALMTATNTTPLRAPTTHTIPVWY
                     GGSAGPDLGDVAKRAALMPEQVAALHASVLYRVYMLGFSPGFPYLGTVPDRIATPRLP
                     TPRKQVAAGSVGIAGTQTGIYPHASPGGWRIIGRTPVPLFSLTRPAPFLLAPGDQVQF
                     VPIRETEFHALFENHT"
     gene            5963..6739
                     /locus_tag="PK224_14435"
     CDS             5963..6739
                     /locus_tag="PK224_14435"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRB16989.1"
                     /translation="MNKERSIDLNCDLGEAATSEQLDVEARIMAYVTSVNIACGVHAG
                     DAALMRSTVQLARQHDLAIGAHPGLPDRDSGGRREQPLSRSLVQDLILSQVGELMAIS
                     QAEGLRLSHVKPHGALYNMSARDPALADAIAEAVAHIDPRLILVGLAGSELLVAGKAR
                     GLPVAAEGFADRAYQADGRLVPRGQEGALIHDESTVVSRAHSLIHDDTIAAIDGSSLH
                     LHIDTLCVHADTPGAVRLAHALRTMFDDGGISTVRIDHAS"
     gene            6699..7694
                     /locus_tag="PK224_14440"
     CDS             6699..7694
                     /locus_tag="PK224_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRB16990.1"
                     /translation="MEGFPQSVSIMPLKQPTIHVVRPGLFTTIQDLGRFGYQRFGVSV
                     SGAMDPWALTVGNRLLGNPDRAAGLEVTIQGPELLFEDALSIAITGADLSPTGNGVAL
                     PMWTVVRMPAGSRLEFGIRRQGTRAYLTVAGGIDGPLMLGSRSTHLRSGLGGVAGRAL
                     RKWDRLDVGPARAAGKPCVGRALPEAQRPRYQSTATIRVIPGPQADRFTDDALERLAS
                     QPYRLASESDRMGYRLQGLALPHRTSADIVSDAVSAGNIQVPADRQPILLMADCQTTG
                     GYAKLATMIAADRALAAQLSPGDRLSFVVVTHETASTLLRSAHAELDRLLPPHRA"
     gene            7828..8028
                     /locus_tag="PK224_14445"
     CDS             7828..8028
                     /locus_tag="PK224_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16991.1"
                     /translation="MDTTLTPRQITHDEKKAAEAAFAGRPFNPKWSDAARIVYDGLLA
                     AMGKDARTVDMHDELEPVEPLA"
     gene            complement(8140..10815)
                     /locus_tag="PK224_14450"
     CDS             complement(8140..10815)
                     /locus_tag="PK224_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB16992.1"
                     /translation="MDLITTHLNADFDGLASMVAARKLYPGAVLVLPGGAQESVRGFL
                     ATHHLDIARLKDVALERVRRLILVDVQEPERLGPLKTLSSRADVEVHVFDHHGADDEP
                     EPQTRPQWPHLAERHIEPVGATTTLLIERLKAQQIILTAAEATVLALGLYEETGSLAY
                     PSTTPRDLEAAAVVLRAGADLNVVAEVLRHPLDPDLIALLNDLLQSGAIYYLEGRKIL
                     VASSTYDRYKGDLAEAVHRLAELQGFDAVIVAIALDEKVEVIGRSRRPEIDVAWIARE
                     FGGGGHAVAAAASVKGRTLIEVQERLTRLLTERYRPTLVARDVMTQPVKTIVADATVA
                     EAERSMTTYGVNVLPVVDQKDRYVGTISREVVQKALFHRLGAQRVEDLARHDQYSADP
                     ETPFHDIEMRMIELNQRFVPVLCGGKTVGVITRTDLLRSLHEDVIASARGKAKSLMDL
                     ESSGAVRRRDVGGLLRDRLPREVHDLLQTAGDLGERLGYSAYVVGGFVRDLLLGIDNL
                     DVDFVVEGDGIAFARALAKERAGRVKVHERFGTAVVLLPDGFKVDVATARTEYYEYPT
                     ALPTVEQSSIKKDLYRRDFTINTLAVRLNPRAFGQLIDFYGGQRDLKERLIRVLHSLS
                     FVEDPTRVFRAIRFELRFDFHLSKETLALIKGAVKMELFHRLSGQRLLDELRYLFSER
                     TPRHAVRRLADLDLLRFIHPTLTWSNRLDRRLSEVEAALDWYKLSCLDRQISGWLVYA
                     MALTEAMPDQAVREMIERFPFTEAEREAITAARFLTQPVCRALSRRVPLRPSETVALL
                     AGLADETLVFLLAKNGSASAKRHLSLYLTTYRDVKPALTGKSLQALGFRPGPLYRTIL
                     ERLTEARLDGDVKTEAEERELVKTFSDQGRGRARD"
     gene            complement(11038..11355)
                     /locus_tag="PK224_14455"
     CDS             complement(11038..11355)
                     /locus_tag="PK224_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16993.1"
                     /translation="MKQVMWGAALLSVCATSLVFAQAETPRIDQRQANQEKRIDQGIA
                     SGQLNEREATRLNNQQEHVNKMEDKAKSDGVVTKKERARISHAQDRTSRHIARQKHDR
                     QQR"
     gene            complement(11499..12242)
                     /locus_tag="PK224_14460"
     CDS             complement(11499..12242)
                     /locus_tag="PK224_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRB16994.1"
                     /translation="MGRRCTYLPAHVVDGMISVVIPAYNEERALPATLRQLFEQAGSY
                     EVIVVDGGSHDRTCEIVRRWPINSRLPAPLTLVTAPKGRASQMNAGAKLARGEWLLFL
                     HADTLLPVDALSRINALERNLTIQAGGFRHRFSGSDWRLRMISWLDNFRCVRSHIIYG
                     DQALFVRRVLFERLGGFPDRPILEDVAFGENLLKVTTPLLLSPPVVTDARKFLKMGIW
                     RSFLRVLLVILHVEFKLPYLPRAFFQDIR"
     gene            complement(12194..12829)
                     /locus_tag="PK224_14465"
     CDS             complement(12194..12829)
                     /locus_tag="PK224_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRB16995.1"
                     /translation="MVVLPGERVPIARLLAFLEHGERMAHGCAQRQATLAPDPKTRRF
                     LTTQARQEAMHAVVFHGAIAWLAPRHLGDAPFLPALEDYRRLLAEALERGDWLESILA
                     EQVILEGLGEAILTRIEGGLEKRQAPFRRLRRVLLHQEEAHHDFGRRVLERAMVDGHR
                     ELESLRLRTQDYLALTDSMVLTLCDLFETIDEDASAWAADVRTFLPMWLTA"
     gene            complement(12841..13614)
                     /locus_tag="PK224_14470"
     CDS             complement(12841..13614)
                     /locus_tag="PK224_14470"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HRB16996.1"
                     /translation="MRKFLIGALMIVLGGCSAVPTSFTPSDPLPPEQLSHRVWHELLG
                     THVRDGVVDYPAIRRQGQLPSYVSLLNRVDPGRLSAGERLAFWINAYNAFAVTGILDG
                     YSPDTLFGRYRYFIAREYSVGGQPVNLYNLEREILIAQFHEPRVHFAIVCASASCPKL
                     QSWAFDGRRLDEQLNRVAIQFVNDPSRNRFDRSRKVASLSMIFKWFARDFEVRSGTLL
                     GFVGEYLQDQSLKQDLLAGGYTVEFLDYDWQLNGLPPHY"
     gene            complement(13628..14629)
                     /locus_tag="PK224_14475"
     CDS             complement(13628..14629)
                     /locus_tag="PK224_14475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB16997.1"
                     /translation="MTRKPSLAMIADCARPLANLVRGRPVLATFQVTLRCNSACGYCD
                     LPLNQGRYELTREEIRGIFGLLYTEGIRFVLVQGGEPLLRPDLVDILEDLSSLGLHVT
                     VITNGTRLTTDLVARFTSLRLPLSVSLDTLDRTRYRQIRGADQLPQVLAGLSLLAQYP
                     YPKFLTCIVSEVNREEVPDVVRFAKARGFIPVVGAYHWNVGLYGKEDTGLMYDRSAAA
                     GVFELLLKEDLIPPGYFRKFVADSAGWLRGQQLEPCDAGRYSIAIDASGEVSPCLAMS
                     SVGNLRELPYREILSRFSGPQIQACSDCSSCNRLDGRVVGTVLRHPLSAWRTPVRFQ"
     gene            14830..15243
                     /locus_tag="PK224_14480"
     CDS             14830..15243
                     /locus_tag="PK224_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB16998.1"
                     /translation="MNTKLQASILTIAAATLATVAFVVAPASAKDKEKAGKHSNHATT
                     TSAAAASSLLRSVPEQPPAPKPGQHDSKPGVAWKTVGGTVKEIHGETYTVEDFDGSQM
                     KVHVGQGTKHLRGNKKVGDTIRAEITHGGFANSIQ"
     gene            complement(15351..16088)
                     /locus_tag="PK224_14485"
     CDS             complement(15351..16088)
                     /locus_tag="PK224_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HRB16999.1"
                     /translation="MMRRGIVLLMCLSGAAMVAHAQGSAKLEVGHFSVAAEGAVLPDG
                     WAPLTFKKIERHTTYEMVKDGPVSVVKAVSEASASGLTKAVAIDPHEYPIVRWRWKVE
                     NLLQKGNVNRKDGDDYPARLYITFAYEPAKVSFGRKLKYQAGRVLFGDIPIGALNYIW
                     DGQSLVGTVVDNAFTDFAKMIVVESGPQRIGTWVEEERNVYEDYRLAFGEEPPAISGV
                     AIMSDTDNTKERAVAYYGDIVFVRALK"
     gene            complement(16097..17650)
                     /locus_tag="PK224_14490"
     CDS             complement(16097..17650)
                     /locus_tag="PK224_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HRB17000.1"
                     /translation="MSASVQSLVLPDDEHNRTLVANVHPPDWLNPEPAGRYNMVVVGA
                     GTAGLITAVVAAGLGAKVALIERHLMGGDCLNVGCVPSKALIRAANAWAQLRDASAFG
                     LHIPPGVTRDFGAVMTRMRKLRAGISHVDSAHRYKSLGVDVYIGQARFTGWDAVSVEG
                     PSGNRTLTFVNAAVCTGARASAPAIPGLAEVGYLTNETVFSLTELPARLAVIGAGPIG
                     CELAQAFARFGSEVSLIEAMHGIMPNEDRDAAEVVQQSMTRDGVRILCCGKDLRVERT
                     ATGKRLLVDSHGQHYDVTVDEILVGVGRSPNVEGLGLETIGVEYDKTGLKVNDRLQTT
                     NPRIYAAGDICSRHKFTHAADAMAQIVIQNALFPHPFGLGYASMESLIMPWCTFTEPE
                     IAHVGLYEADAKKKGLEIETYTYKLGEVDRAILDGEQEGFARIHIQKGTDKILGATIV
                     AAHAGDLISEFSVAMKAGAGAKTIAATIHPYPTRAEVNKKVVNLWRKAHFTARTKMLL
                     TRLFAWLRR"
     gene            complement(17700..18437)
                     /locus_tag="PK224_14495"
     CDS             complement(17700..18437)
                     /locus_tag="PK224_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HRB17001.1"
                     /translation="MIPPQGAMTTSVASPSSSKRAGLGKLMLGIFVGLSLAAFFYFDL
                     GQYLSLDGLKSNRARLLAFTEANYPVAVAMFVLGYCVVVGLSLPGGAIMTLAGGFLFG
                     SLLGTLYVNVGATVGATLAFLVARYLLREWVEQKFGSRLDAIQQGFARNAFSYLMTLR
                     LIPLFPFFLVNMVSGLTRVNVGTYMAATSLGIIPGSFVFAYAGRQLGTISSLKEIASP
                     NVLLAFTLLGLLALVPILYQKFAGKAV"
     gene            19009..19158
                     /locus_tag="PK224_14500"
     CDS             19009..19158
                     /locus_tag="PK224_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17002.1"
                     /translation="MGPLSVHDMKSGFFLFASFLLLATICAIAALGTLMRPQRWSERE
                     TRLHV"
     gene            19407..20051
                     /locus_tag="PK224_14505"
     CDS             19407..20051
                     /locus_tag="PK224_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HRB17003.1"
                     /translation="MDQNDRFIKTKENWAKARRGGEEREVFYEGDDRLPPGQHLVETW
                     PVLDLGQKPDIPLSEWTLTIGGAVTTPATWTWADFLAQPQFKNVSDFHCVTSWSRYDN
                     EWEGVSFKQLMAVVQPLPSAKFILFKSYDDYTTNLPLVACQDDDVLLTYKWNGRPLTK
                     EHGGPVRMIIPKRYAWKGAKWIKEITFSDHDEKGFWEVRGYSNSAFPWQNDRYG"
     gene            20048..21169
                     /locus_tag="PK224_14510"
     CDS             20048..21169
                     /locus_tag="PK224_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRB17004.1"
                     /translation="MNEHNSPVNAPIIQRIEIWPVDIPITDPFVVATGARVTAQNLFM
                     RVTLRNGTQGVGEAAPFPEVGGEDRATCFAAATELAKTMIGQSVGDYESLADRLNEQA
                     TLHPAARCGLETAMLDAYCRSRRIPLWQLWGAADVRERETDITIPICSPEKTLELARG
                     WYARGFRLFKMKVGTDVEQDIRRLQAVHNALPEIGFIGDGNQGFSREDCLRFVHGVKQ
                     FGGRLVLLEQPVVRDDLEGLQAIRHLTGIPVAADESVRSLDDAREVVRMQAADYINIK
                     IMKTGVIDAWRIAAFTRSAGLRLMVGGMLETRIAMGCSFSLVLGLGGFDVLDLDTPLL
                     LSTDPVTGGYRYSGPRLQPWSEGGLDMQIPCPADRITVQ"
     gene            21361..22164
                     /locus_tag="PK224_14515"
     CDS             21361..22164
                     /locus_tag="PK224_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17005.1"
                     /translation="MQVITPRPILATASPRNIRPIALFWMLGVGTLMLGGCVSQQTYE
                     TARHEVKTRATELAQTQADIQSLEQQREETHLANQREERSLATLKGELKKIQLAYEQL
                     HKSNQAKLAALEYSIVALRARHQAMLKEISETKRIEKRLEALTAQHEHTMATTPSGPE
                     ARVTTVDGSPQEPHLVAVITPQTPQIDSTPTASQAPPAATSAATTPAVPQPAPVVATP
                     PSPSTPAKAAQAPAAPQAPRPATAAAPQDESWFSSVTGWFTSLFDWIWA"
     gene            complement(22238..22459)
                     /locus_tag="PK224_14520"
     CDS             complement(22238..22459)
                     /locus_tag="PK224_14520"
                     /inference="COORDINATES: protein motif:HMM:NF023510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HRB17006.1"
                     /translation="MQQAIIGYHQDDEGHWVADLRCGHGQHVRHQPPMTSRPWVLTEE
                     GRRAFLGTELNCKKCEEGGDGFDPTGAQS"
     gene            22600..23499
                     /locus_tag="PK224_14525"
     CDS             22600..23499
                     /locus_tag="PK224_14525"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB17007.1"
                     /translation="MAQSEFRIGIVGIGRMGANMARRLHDLHYAIVAVYDTDTERAGA
                     LAKELACTAVTTPARVAELADTVLTVVSDDAAMRQIYSLGGPDSLLRHAANRLFINCA
                     TLSPELQREVHALVEQQGGRILEACMASSITQARQGTLYLMCGGRPEVFERAKPLLQD
                     LSAHLRYIGPAGEAAKVKALVNMVMNANTAALAEGLGLGSALGLDLTMLREVFSQTGA
                     NSRVLETDGEDMQQRAHDCYFSAAHAAKDSGIACALAQQAGLDLPLAKATYDQYRRLI
                     DHGKGELDKSAVAELTFKDRLNH"
     gene            complement(23609..24073)
                     /locus_tag="PK224_14530"
     CDS             complement(23609..24073)
                     /locus_tag="PK224_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17008.1"
                     /translation="MMRGRGRGPGWALIGLVLLLSACNSDRPEPAEFDLPSAGLRLNL
                     TRLATHPFLSRYRLTLRIEGSRGCSVTAELFPDTGYAGRRNVYQQPSGVITVLGQYDA
                     RVFDPANCSLRLVEFQSLVGQATFLGAFDVDAQKRWRFLDASVRPERSFEKH"
     gene            complement(24070..24621)
                     /locus_tag="PK224_14535"
     CDS             complement(24070..24621)
                     /locus_tag="PK224_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRB17009.1"
                     /translation="MVGTIQLLKRGMGRVGMIAGVLFLAGGWPVVASAADELPKEAVL
                     PAALAGKAAQAALDFCKKDGYRVSASVVDRAGVLRAMMRADGAGPHTVDSSRKKAYTA
                     ASLRRATSDLADMIAKQPALQALREMNESILMVGGGLPIEIAGEVVGAIGVGGAPGTH
                     LDDACAEAGLDAIGAASKMPTAK"
     gene            complement(24765..25751)
                     /locus_tag="PK224_14540"
     CDS             complement(24765..25751)
                     /locus_tag="PK224_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17010.1"
                     /translation="MKRVGGWHTLWRGKTCLQLVRGVMVYVWPLSILGRLFYRLRWSP
                     PLYGAINRRSRRVFRSIPQVLGPHGVETVRALETDGIFRTSVEALAQDPALFRDLARD
                     AGMMLAGQDVQRKIRQRYNPNDAKWYVVRAIGRRAKRPPIPSSFIRFFLHPQLLSIAS
                     TYLGLQVRLNYMDVWHNIPVCEGEPPISAELWHRDHEDKRIIKVFVLLSDVDGTMGPF
                     TYVKRSQVGGEYGSLFPAIPPTGRYPKQDVLDQLINDTPLPSVSCVGPAGSVILCDAS
                     GLHRGGRSLTHPRIVLVGVYTSNAGLDAARYSLPRSVGHEQLSPAAKFALYS"
     gene            complement(26177..26683)
                     /locus_tag="PK224_14545"
     CDS             complement(26177..26683)
                     /locus_tag="PK224_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17011.1"
                     /translation="MRQHTSVTIVALLSAGLFLTGCQLFDSSTRQPAPRSASVMSMWE
                     LYRHCQSSGDVETVLSAAKQLQQSADTHVVPAPDVPKSLDRFVTRQPVRTTVDPKALA
                     ASCTLQAARTSLSAGREQEAEQLLYAVVLSYPESDYTFYVAQARVWIEELHRPGTLDA
                     VIHPISTF"
     gene            complement(26826..28811)
                     /locus_tag="PK224_14550"
     CDS             complement(26826..28811)
                     /locus_tag="PK224_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRB17012.1"
                     /translation="MTIDSRRCQDLDRALSLEWLETNGRGGYASGTVAGANTRRYHAL
                     LLVARRPPVDRVVLVNHLEESVEVGGQSFPLSTNLYSGAVHPEGYRYCDGFSALPWPT
                     WRYASHGIRLERELLCPDGRDMVVVRWRLLGDAPSAVVLRVRPMLSGRDFHALHQENA
                     GVRSQATVHEGRVTWQPYDALPAVLAFHNGEYHHGPDWYRHIHYRIERERGLDHTEDW
                     WSPGECEFTLTPGSVAELLFTTEQLESVDVAQVVAAERRRRAECPVSAPTDDALAQEL
                     WVASGAYLVRRGNGQTVIAGYPWFADWGRDTFIALPGLCLVTGRYDVARQVIEAFASH
                     VSQGMVPNRFPDIGEQPEYNTIDASLWFIHAVDRYLHYSRDLDGVRRIAWPAIKQIVE
                     GYRRGTRFGIHMDDDGMITGGAEGVQLTWMDVKIGDWVVTPRHGKPVEIQALWVRALA
                     VAAELAVQFGEPAYSAQCRQDRARATESFRARFWYRTGGYLFDVVDGPIGDDASLRPN
                     QIYALALDDQLVTEAQAKHILQLVRERLLTPLGLRTLAPEDIRFCASYEGGVSERDGA
                     YHQGTVWPFLLGPFVTAWVNTYGDSPAVRRDARMFLDGLHEHLHEACLGHVSEIFDGQ
                     HPHRARGCVAQAWSVAEPLRALIEDLGAVHERSSSPR"
     gene            complement(28826..31501)
                     /locus_tag="PK224_14555"
     CDS             complement(28826..31501)
                     /locus_tag="PK224_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HRB17013.1"
                     /translation="MSTSPGEEAVRPGSAEQQRLDEDDLRRKHWKRWGPYVSERAWGT
                     VREDYSSYGTAWESVPHDHARSKAYRWNEDGLAGICDRHQFVCLALALWNGRDPILKE
                     RMFGLTGNEGNHGEDPKEYYFYLDSTPTHSYMKFLYKYPQAAFPYARLIEENRRRGRT
                     DPEFELLDTGVFDDDRYFDVGVEYAKASPEDLLMRIQIVNRGPDPAQLTVLPTIWFRN
                     TWSWGLDVRRPRMRKGESTSSVSIVEFDHEYYGPRRLLCEGGPDLLFTENETNTRRLY
                     GDVDGARQVKDGINDYVVQGDKGAVNPDQIGSKASAHYVVTALPDRPVTIRLRFTNGA
                     AEGALDPQAFDAVFAARLKEADEFYAALAPANVSEDARMVQRQAFAGLLWSKQFYHYD
                     VDRWLKGDPAGPEPPKERLHGRNADWAHLYNADVISMPDKWEYPWYAAWDLAFHCLPI
                     ALVDSRFAKDQLVLMLREWYMHPNGQIPAYEWALGDVNPPVHAWAAWRVYKIERKRKG
                     VGDRIFLERVFHKLLLNFTWWVNRKDAEGKNIFQGGFLGLDNIGVFDRSKPLPTGGHI
                     EQSDATSWMGMYCLNMLSIALELAREDRAYEDVASKFFEHFVYICRAMNNIGDAKIEL
                     WNKADGFFYDVLHLPDGKKFPMKVRSMVGLIPLFAVETLDSELIDRLPRFKHRMQWFI
                     ENRPDFSLHIETQSQDGEVRRFLSLVNRHRLPKVLRYMLDEQEFLSPYGVRALSRYHK
                     DHPYVFSMMGTSQSVEYQPAESTNGLFGGNSNWRGPVWFPVNYLLIESLQKFHYYLGD
                     GFTVEYPTGSGQKRTLADVAAELSRRLTHTFLRGPDGRRPVYGGAEKFQQDPHWRDLI
                     LFYEYFHGDNGAGIGASHQTGWTGLVAKLIQQSGE"
     gene            complement(31824..32009)
                     /locus_tag="PK224_14560"
     CDS             complement(31824..32009)
                     /locus_tag="PK224_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17014.1"
                     /translation="MASFRFDLEKRSTDGIPNLAELESGKLLGAILPMSEQAQLQLRD
                     SIEVIDYLMNQEGVVWS"
BASE COUNT         6747 a        10379 c         9056 g         5984 t
ORIGIN      
        1 tccgtcgagg aggccgaacg agaacagccc cgccgggcga gaggatcgga cgacggagca
       61 gacagtcatg aatcgtccgg gctaaggccc ctctgaggga cagaccgtga actacacggt
      121 gataccaccg agaccaacaa cgacaaccgg caccgacgat gactacggat tcccagtatc
      181 aggactttgt tccgccgcat cgcctgctca tgggcccggg cccgagcatg gtccatcccc
      241 gggtgttgca ggccctgtca cagccgttgc tcggacacct cgacccggtc tttctcactc
      301 tgatgaacga cattcaagcc ttgctccgcg ccaccttcca tacccaaaac gagtttacga
      361 ttgccttgtc cggaaccgga tcggcaggca tggaagcggt gatcgccaat ctcatcgaac
      421 ccggcgatcg tgcgattgtg ggagtcaacg gcgtgttcgg caccaggctg gcgaccatga
      481 tcgaacgaag cggcggcctc ccgatccgca tcgaagcgcc ctggggagcg acgattccca
      541 ttgaggcgct ggaacaggaa ctggcgcggt ctgctccggt caaagccctg gtcctggtcc
      601 atgcggaaac ttcaaccggc gccctccaac cccttgagga cgtggggacg ctgtgccgcg
      661 cgcacgatgc attattgatc gtcgacgccg tcacctccct gggaggcatc ccggtcgagg
      721 tcgatacctg gggaatcgac gcctgctaca gcggcacgca gaagtgcttg agctgtcccc
      781 caggcctggc cccgctcacc gtcagcccgc gaggcatggg cgccatccgg cagcgacgtg
      841 ctccctgcca cagctggtac ctcgatctgt cgctcatcgc cgactattgg aacgaccaca
      901 gccgtgccta tcaccacacc gccccgatct cgatgctcta cgggcttcgg gaagccctgc
      961 ggctggtgca cgaagaggga ctcccggcgc gtgccgcgcg acatcaggta aacagtgatg
     1021 cattgatggc cgggctccaa actctgggcc tgaatcccct gcccccgccg ggccatcgac
     1081 tcccgatgct caactgtgtc acgctacccg acggaatcga cgaggcgacg atgaggacgc
     1141 aactgctgca acagtacggc atcgaaatcg gaggcggact gggaccgtta cggggacggg
     1201 tctggcgcat cggcctcatg ggcgaatcgt gccgacaagc gcatgtgctg acgctactca
     1261 acgccctgga agaaatcttc gcaggccagg gacggccggc gcagccgggc cgcgccgtcc
     1321 gggcagcggc agaaacctat acccattcac taggatcggc gaggaggggt gcatgagtcg
     1381 aaacgggtta tggatcgtgg cggcaggagt ggccggcttc gtggcagcca tcacctactg
     1441 gatcgtcgcc ctcgcggtcg cagaatcaca caaggcggag atggtccccc ccgagcgtgt
     1501 gaccagcttc attcatgcgg tcatcgacgc gaatcgagcc aactacacac agaacgtcgt
     1561 ggacaagctc cataaccagg gcgtcgtcga ggcactcgaa cattggaaag aagagaaggg
     1621 cctcccgctc ccggcccaat tcctcctcga atccgggcgc ctggtggcgc aaaaggatat
     1681 gaaactcagt ttccgtctgg ccagcctcac cccgatatat gtgtggaacg gaccgaacag
     1741 cgagttcgaa cgacgcgggc ttgaggtcgt gatgaaggcg cccgaaaaac ccttcaccgg
     1801 attctaccaa caggcgggcg tccgctactt ccaaggcatc tacgcagacc gggcggtgtc
     1861 cgaaagctgt gtgagctgcc acaatgccca cgtaaacagc ccgcgacggg actacaaact
     1921 caacgacatc atgggcggcg tcatcgtcac cattccgatc agtgaggcgc catgaccatc
     1981 gccatccgcc acatccgcat tctggacggc ctcggtcacc ggattgagcg aggtaccctc
     2041 ctcatcgagg gcacccgcat ccaggccgtc gggccggaac aggacgttcg actccccaag
     2101 ggcgcgcaac gaatcgacgg ccgcggcctg accgtccttc ccggcctgat cgattgccat
     2161 gtccactttt gtttgggagc ggaagcggat gtcgtggctg cggtggaggg agaaccttcg
     2221 tccctcaccc tgctcaaagc cgccgagctg gcgcgccgca ctctccacgc cgggttcacc
     2281 accgtccggg atgtgggatt ccgggaccat gcggtgttcg ccctgaaaca ggccatcgag
     2341 tccgcactga cgccggggcc caggattctc gccgccggtc tcgcgatctg catgcctggc
     2401 ggacatgcgc gcttcatcgg ccgcgaagcc gacggagtcg aggccgtccg cgccgccgtc
     2461 ttggatcaac tcaatgccgg cgccgaagtc atcaaagtca ttgcctccgg cggcgtgctc
     2521 acccccggca cctctccgga cgacgcgcaa atgaccgtgg cagaactgtc cgctgccgtc
     2581 gaagtggccg tcgcccacgg gcgacatgtg gccgcccacg cccacggcgc aaccgggatg
     2641 aagaacgcgc tccgcgcagg cgtccactcc atcgaacatg ccaccctcat ggacgacgac
     2701 gcggcagaga tgatgacgca acgccacgtc tacatggtac ctacgctgtc ggccctcgcc
     2761 accacagcgg cctgcccgac cggctgcggc attcccgaca gcgcccgctc caaagcgagg
     2821 aacatggtga agcagcacga gagaagtttc cgcgccgccc tccgccgggg tgttccgatc
     2881 gccatgggca cggatgccgg aacgccgttc aactatcatg gggacaatgc tcaggaattg
     2941 gaacggatgg tcgcgctggg catgacgccg atggaagcga tcatgaccag cacctccgcc
     3001 gccgcgcggt tactgggcct gattcaagac atcggatcca tcgcggcggg aaaacaggcg
     3061 gacctgctgg tcgtggaagg caatcctctc agaaatatcg gagtactgct gaaacgagag
     3121 cggattgcgg gagtgatgca acgcggtcgg ttcgtggcgg gtccgctctc acgagcgtga
     3181 aacgtgacac gaaagacgtg ggtcgttacc gtctttcata cagtaattcc aaggccgtcg
     3241 cgaagccgtt gatccggccc ttgcggaaca tttcctctaa ccggccgcga agcgcacgaa
     3301 tcccgccctc gtctcccttc gcgaccaccg cctgcgacac catcatctgc accgcgacct
     3361 caagcaagcg ctgtttccgt tgctccatat cggaagtcac gaactcatcc aggacttccg
     3421 ccccgcgcgc ttccacgatg gactccttga aggtgtcata cccctgatgc gccaccgtgc
     3481 gttcacgaat gagggccagt tccgatttga tccgtggcac catcttcatc aggacggcga
     3541 agacttcctt ccgcccttcg tcaaagagct ttttctccat ctcctcaagg atcagggacg
     3601 gatccaccgt ctcggcccgc gcctcttctc cccaatgcag gcgatcatag gttctccggc
     3661 tctcgggatc gcccaggacc tcataggcgg agttgatctc gcggatcttc tcctctgcct
     3721 gcttgttgtg gggattgcgg tcgggatggt gctcgaaaac gagcttccgg taggcttttt
     3781 tgatgtcgtc gtcggaaatg tcgcgcgaca caccgaggac acgataataa tccaggcgag
     3841 gcatacaggg cgagactata ccatgccgct tcgccggctt caccttgaca gcgtcgggac
     3901 tcccccgtag cctggcgcgg aggcagcctg ttcgcagggg agggagttcg gcatggcgtg
     3961 ggcacaaggc gtcacgaggt atcaatggct ggtcctgttc gtcgcctggc tgggttgggt
     4021 attcgacgcc atggacgcaa cgatctacgc gatcgtgctg catcctgcct tacatgagct
     4081 tctacaagcc ggggccgcca gcggagccgt cacgaacgaa cggatcgggt ggtacggcgg
     4141 catggtgttt tctattttcc tgatcgggtg ggccatcgga ggaatcggct tcgggatggc
     4201 cgcggactat ttcggccgga ccaagacgtt gatcatcacg attttgatat atgccgtctt
     4261 caccggcctc gccgcgtttg ctcaagaatg gtggcatctg gccatctatc gctttctgac
     4321 cgcgctcggc atcggcggcg aatgggccgc cggcgcggcc attgtggccg agacctggcc
     4381 tgaagacaaa cgtgcccggg cagccgggat tctacagtcc gcatgggccg ccggattttt
     4441 cctggccgcc ggcttcaacc tgctcctgag cgactacgga tggcgactcc tcttcctcgt
     4501 gggcatcctg cccgcctttg tggccttact cgtccgttgg tgggtgaaag aaccggagcg
     4561 atgggtgcag gcccacgaac aggaggcaca atccgttcgc aacagcttca tgcacctcgc
     4621 cgagttgttt caaccggcat tacgccgcaa cacctgggtc ggctcaaccc tggcgttcgt
     4681 ggcggtcttc ggcctgtggg gcgcgaccaa ttgggccccc accttgatcc gggccatgcc
     4741 ggacctccag gggcaggatg ccgccacgct gacacagaag gtcagttacg cgatcatggc
     4801 gctcaatgcg ggctcgttgt tcgggtatct gggattcggg ccgttggccg aacggttcgg
     4861 ccgcctgccc gtattcggct tgatgtgcct cggcagcttc atcatgctgc cggcgaccta
     4921 ttttgtcccg cacagttatg cagaagtgtt gctgttactc ccgctgctgg ggttcttcaa
     4981 caatggcatt ttcagcggct ttccgattta cttgcctgag ctctatccga cacgactccg
     5041 tgcaaccggc tcaggatttt gcttcaacgc aggccgcgtg ctcgcctcgg ccgcgccctt
     5101 cgtcaccggg tggctcgtca ccctgctggg atcgttcaac aacgcggcga gcaccatcgc
     5161 gctgatctat ctgttgggat tggccgtact gcccttcgcg accgagacga atggaaagcc
     5221 gttaccggag tgacatctcg cggaccgact ccccggctat tatgaaacaa cctgagactg
     5281 cgctcccgcg catcgtgcca ctgagtgagt ccgccctgac cattgaattc ggggagacca
     5341 ttgacccaca cacgaatgag tcggtcctgg ccttcgccac cgccgtggaa cgggccgggc
     5401 tcccgggcat tcttgaggtg gtgccgacct accgttccgt cacggtctat ttcgatccgt
     5461 tccgtaccga cgtggccacg atgaccgggc atttccgtgc cttgatgacg gcaacgaaca
     5521 caaccccgct acgtgccccc acgactcata ccattcccgt gtggtatgga ggtagcgcag
     5581 gacccgatct gggcgacgtg gccaaacgag cggcactcat gcccgagcag gtggcggccc
     5641 tgcatgcttc cgtgctgtac cgcgtctaca tgctgggatt cagcccgggc tttccctatc
     5701 tagggaccgt gccggatcgc atcgcgacgc cgcgactccc gacgcctcga aaacaagtcg
     5761 cagcaggatc ggtcggcatc gccggcacac aaaccggcat ctacccgcat gccagcccgg
     5821 gcggctggcg cattatcggt cgcactccgg taccgctgtt cagcctgacc agacccgcgc
     5881 catttttgct ggcaccgggc gaccaggtgc aattcgtgcc gatcagggaa acagaattcc
     5941 atgcgctgtt cgagaatcac acatgaacaa ggaacgtagc atcgacctca attgcgacct
     6001 gggagaagcc gcgacttcgg agcaactgga tgtggaagcc cggatcatgg cctacgtcac
     6061 atcggtgaat atcgcgtgcg gggtgcatgc aggcgatgcg gccttaatgc gaagcaccgt
     6121 gcagctggct cgacagcatg atctggcgat cggagcgcac cccggattgc cggatcggga
     6181 ctctggcggc cgtcgtgaac aaccgctctc ccgttcgctc gtacaggact tgatcctgtc
     6241 ccaagtgggt gagctgatgg ccatcagtca ggccgaaggt cttcggctct cgcatgtgaa
     6301 accgcatggg gcgctctaca acatgtctgc tcgcgacccc gcgctggccg acgccatcgc
     6361 cgaagcggtc gcacacattg atccgcgatt gatcctcgtg ggattggccg gatcggagct
     6421 cctcgtcgcc ggcaaggccc gcgggctccc ggttgccgcc gaaggatttg ccgatcgggc
     6481 ttatcaggcc gacggccgtt tagtacctcg agggcaggaa ggcgcgctca tccacgatga
     6541 atcgaccgtg gtctcgcgcg cgcactcgct gattcacgac gacaccatcg cggcgatcga
     6601 cggttcctcg cttcatctac atatcgacac gctttgtgtc cacgccgaca ctcctggcgc
     6661 ggtccgactg gctcatgcgc tacggacgat gttcgacgat ggagggattt ccacagtccg
     6721 tatcgatcat gcctcttaag cagccgacca tccacgtcgt acgccccgga ctcttcacga
     6781 ccattcagga tctcggtcgt ttcggatacc aacggttcgg cgtgtcggtg agcggcgcga
     6841 tggatccttg ggcgctcacg gtcgggaatc gcctgctcgg caatcccgac agggccgccg
     6901 gcctggaggt caccatccaa ggaccggaac tgttgttcga ggacgcactc tccatcgcta
     6961 tcaccggggc agacctctcg cccaccggca acggcgtcgc actgcccatg tggaccgtcg
     7021 tccgcatgcc cgctgggagt cgccttgagt ttggaatacg ccgacaaggt actcgtgcct
     7081 acctcaccgt ggccggcggc atcgacgggc ctttgatgct cggcagccga tcgacccatt
     7141 tgcgcagtgg attgggaggc gtcgcaggcc gcgcgctgag gaagtgggat cgactcgatg
     7201 tcggaccggc acgagctgca ggcaaaccat gcgtaggacg agcactgcct gaggcacaac
     7261 gcccccggta tcaatccacc gccacgatac gggtgatacc ggggccacag gccgatcgtt
     7321 tcacggacga tgcgctggag agactcgcga gccaaccgta ccgactcgcc tccgaatcag
     7381 accggatggg ctatcggtta caaggattgg cgttgccgca tcgcacctcg gccgacatcg
     7441 tgtccgatgc cgtgagtgcc gggaacattc aagtgccggc ggatcgacag ccgattctac
     7501 tcatggccga ctgccagacg acgggggggt atgccaaact cgcgacgatg atcgcggccg
     7561 atcgcgctct cgcggctcaa ctatcgccgg gagacagact ctcgttcgta gtggtcacgc
     7621 acgagacagc atcgacactg cttcggtcgg ctcacgccga acttgaccgg ctcttgccgc
     7681 cgcaccgtgc ttgaggagat tcccttagtc cggacgcttc aggccatgca tgccgcagca
     7741 ctcgaaagca atgcccgcta aacaacgggg gttctgccat tccgttgagt ccttgacatt
     7801 gccctgaagc gatctattat tattcgtatg gacaccacac tgactcctcg acaaattact
     7861 cacgatgaga aaaaggcggc cgaagccgct ttcgctggtc gcccattcaa tcctaaatgg
     7921 tcggacgcgg ctcgcatcgt ttatgacggc ctcctcgccg caatgggaaa agatgctcgg
     7981 accgtcgaca tgcacgacga actcgagccg gttgagccgt tggcttaacc tccccgcgtc
     8041 gtgtcatcgg gcacgggagg cgttcagcac gacctctcga ggttgtctgc gctctgcggc
     8101 accgccgatg tggcttgata cctccccgca tgcgggaagc tagtctctcg ctctcccccg
     8161 gccttgatca gaaaatgttt tcacgagctc ccgctcttcc gcttcagtct tcacgtcgcc
     8221 atccaacctt gcctcggtca gacgctcgag aatcgtgcga tagagtggac ccggtctgaa
     8281 tccaagcgcc tggagggatt tcccggtcag ggcgggtttc acgtcgcgat acgtcgtcag
     8341 atacagagac aggtgccgct tggccgatgc cgagccgttc tttgccagca gaaacacgag
     8401 cgtttcgtcc gccagaccgg ccagcagagc caccgtttcc gaaggcctga gcggcacgcg
     8461 ccggctcaga gcccggcaca ccggctgcgt aaggaaccga gccgccgtga tagcctctcg
     8521 ttccgcttcc gtgaagggaa atcgttcgat catctcgcgt acggcttgat ccggcatcgc
     8581 ctcggtaagt gccatggcat acaccagcca tccgctgatc tgccgatcca gacaggacag
     8641 tttgtaccaa tccagcgccg cttccacttc gctcagccga cgatccagtc gattcgacca
     8701 cgtcagtgtc ggatgaatga accgcagaag atccaggtcc gccaaccggc gaacggcatg
     8761 gcgtggggtg cgctcagaaa ataagtaccg cagttcatcc aacaaccgct ggccggacag
     8821 tcgatggaac aactccatct tcaccgcgcc tttaatcagc gccagcgtct ccttgctcaa
     8881 atgaaaatcg aaacgcagct cgaagcgaat cgcgcgaaag accctcgtcg gatcctccac
     8941 aaaactcaag ctatgtagta cccggatgag gcgttctttg agatcgcgtt gtccgccgta
     9001 aaaatcgatc agttggccga acgcgcgggg attgaggcgt acagccaacg tgttgatggt
     9061 gaagtcccga cggtagaggt ccttcttaat ggagctctgc tcgaccgtcg gcaacgccgt
     9121 cggatattcg taatactccg tgcgggccgt agccacatct accttgaacc cgtccggcag
     9181 gagcacaaca gccgtcccga atcgctcgtg gaccttcacc cggcctgccc gctccttcgc
     9241 cagggcgcgt gcaaacgcga tcccgtcgcc ctccaccaca aaatcgacat cgaggttgtc
     9301 gatgccgagc agcaggtcac gcacaaatcc gccgaccaca tatgctgagt agccaaggcg
     9361 ctcgcccaag tcgcctgccg tctgcagcag gtcatgcact tcacgcggca agcggtcccg
     9421 caggagtccc ccgacatcgc gtcggcgcac tgctccggag gactccaggt ccatcaacga
     9481 tttggctttc cctcgcgcag acgcaatcac gtcttcatgg agactgcgga gcagatcggt
     9541 tcgggtaatc acgcccaccg ttttgccgcc gcacagcacc ggcacaaacc gctgattcaa
     9601 ttcgatcatc cgcatttcga tgtcgtgaaa gggggtctcc gggtctgcac tgtactgatc
     9661 gtgtctggcc agatcctcga ctcgttgtgc tccgagtcga tgaaacaatg ccttctgaac
     9721 gacctcacgt gaaatcgtcc cgacatagcg gtccttctga tcgacgaccg ggagaacgtt
     9781 gaccccgtaa gtagtcatgg agcgttcggc ctccgccacg gtcgcatccg cgacgatcgt
     9841 tttgaccggc tgtgtcatga catcccgagc caccagggtc gggcgatagc gttccgtcag
     9901 gagacgcgtc agccgttcct gcacttcgat gagcgttcgg cccttcacgc tggccgctgc
     9961 cgccacggca tgtccgccgc cgccgaattc gcgcgcaatc cacgccacgt cgatctcggg
    10021 gcgccggctc cggccaatca cctcaacctt ctcatccagt gcaatggcaa cgatgacggc
    10081 atcgaatccc tgtaattccg ccaaccggtg gacggcctcc gcgagatcgc ccttgtaacg
    10141 gtcatacgtg ctggaggcca caagaatttt gcggccctcc agataataga ttgctccgga
    10201 ctgcaacaga tcgttcagca gggcgatcag atcgggatcc agcggatgcc gcaagacctc
    10261 cgccacgacg ttcaaatccg cgccggcgcg cagcacaact gccgcagcct ccagatcgcg
    10321 cggagtcgtc gacggatagg ccaatgaccc tgtctcctcg tacagaccaa gcgccaacac
    10381 cgtcgcctcg gcagccgtga ggataatctg ctgcgccttc aggcgttcga tcagcagcgt
    10441 ggtcgtggcg ccgaccggtt cgatgtgccg ttcagcgaga tgaggccact gaggtcgagt
    10501 ctgcggctcc ggctcatcgt cggctccatg gtgatcgaaa acatgcacct ccacgtctgc
    10561 gcgtgaactg agggtcttca atggacccaa ccgctccggc tcctggacgt ccacgagaat
    10621 caaccgtcgc acccgttcca gggccacgtc cttgaggcga gcgatatcca gatggtgcgt
    10681 cgccagaaaa cctcgcaccg actcctgcgc accgccgggc aataccagga ccgcccccgg
    10741 atagagcttc cgggcggcca ccatcgaggc cagtccatcg aagtccgcat tcagatgtgt
    10801 cgtgatcaga tccatgccgg cattctaaca ggatcgagcg aatcgattca gtcccgcggt
    10861 ggagacagag gagaaggaag attgtgcaca aagcgagagg attggctgga gaggaaccct
    10921 gccgtccaca cttcttgtgt ggacggcagg gggttactcg acagaatgag cgagcgcgga
    10981 tgcctggctg ggccgaaccg gtcgcccgac cgctcaccac gacatctgtg ataccatcta
    11041 ccgttgctgg cggtcgtgct tctggcgagc aatatgacgc gacgtgcgat cctgtgcgtg
    11101 gctgatccgg gcccgctctt tcttcgtcac cacaccgtcc gatttcgcct tgtcttccat
    11161 cttattgacg tgttcttgct ggttgttcaa gcgagtggct tcacgctcgt tcaactgacc
    11221 gctggcaatc ccctgatcga tccgtttctc ctgattggcc tgccgctgat cgatcctcgg
    11281 cgtctccgcc tgcgcaaaca ccaacgacgt ggcacacacc gacaacaacg ccgcgcccca
    11341 catcacctgc ttcatacatc ctcctttggt tgccccccgt tgcactgtca tccgttccaa
    11401 cgtacaagga cgccggctgg ttgacaatca aaatgggggt ttgaagggaa gtgcggagaa
    11461 ccaatgtcac ggacagacca cgcgagatgg agatgaaatc agcgaatgtc ctgaaaaaag
    11521 gcgcgtggga gatacggcag cttgaactcc acatgcaaaa tgaccagcaa gacgcgaaga
    11581 aaactccgcc agattcccat tttgagaaac ttgcgggcat ccgtgacgac cggtggtgag
    11641 agaagcagcg gcgtggtgac tttcagcaaa ttctcaccga acgccacatc ttccagaatg
    11701 ggccgatccg gaaatccgcc aagccgctcg aacaacaccc tccgcacgaa taaggcttga
    11761 tcgccgtaaa tgatatggct cctgacacat cgaaaattgt cgagccatga gatcatccgg
    11821 agccgccagt ccgaccctga gaatcggtgt cggaatccgc cggcctggat ggtaagattc
    11881 cgctccaacg cattgatccg actcagcgca tcgacgggta acagcgtatc ggcatggaga
    11941 aagagcaacc actcgccccg cgccagcttc gcgccggcat tcatctggga cgctcgtcct
    12001 tttggcgccg tcacgagcgt gagcggggcc ggcaatcgac tattgatcgg ccagcgccga
    12061 acgatttcgc aggttcgatc gtggcttccc ccatcaacca cgatcacttc atacgacccg
    12121 gcttgttcga acaactgtcg gagtgtggct ggcagcgctc gctcttcatt gtaggcggga
    12181 atgacgacgg agatcatgcc gtcaaccaca tgggcaggaa ggtacgtaca tcggcggccc
    12241 atgcagacgc gtcctcgtcg atcgtctcga agagatcgca gagcgtcaac accattgagt
    12301 cggtcaaagc cagatagtcc tgcgtgcgca aacgaagcga ctccaactcc ctgtgcccat
    12361 ccaccattgc tcgctccaac acacgccgcc cgaagtcatg atgagcctct tcctggtgca
    12421 gcagcactcg gcgcaaccgg cggaacggcg cctgccgctt ttcaaggcca ccctcgattc
    12481 gggtcaaaat cgcctccccc agtccttcca ggatcacctg ttcagccagg atactctcca
    12541 gccaatcccc tcgttcgagc gcctccgcga gaaggcggcg atagtcttcg agtgccggca
    12601 gaaagggcgc gtcaccgaga tgacgcggtg caagccacgc aattgcgcca tggaagacca
    12661 cggcatgcat cgcctcctgc ctggcctggg tcgtcaagaa acgacgcgtc ttcgggtcgg
    12721 gagccagtgt ggcttggcgc tgcgcacacc catgcgccat tcgttcgcca tgttcgagga
    12781 atgccagcag ccgggcaatg ggaactcgct caccgggcaa caccaccatg cgcatctcct
    12841 tcaatagtgc ggaggcagac cgttcagctg ccaatcgtag tcaaggaatt ccaccgtgta
    12901 accgccggct aagaggtcct gcttgagcga ctggtcctgt agatattcgc cgacaaagcc
    12961 caggagggtt ccggaccgga cttcaaaatc ccgagcgaac catttgaaaa tcatcgacag
    13021 cgaggccact tttctcgatc ggtcgaaacg attgcgcgaa ggatcgttca caaactggat
    13081 tgcgacccga ttcagctgct catccaatcg acgcccgtcg aacgcccacg attggagttt
    13141 cgggcaggag gctgacgcgc agacaatggc aaaatgcacc cgcggctcat ggaactgcgc
    13201 aataagaatc tcccgctcca gattgtagag attcaccggc tgtccgccga cggagtactc
    13261 ccgcgcaatg aagtaccggt atcggccgaa taacgtgtcc ggagaatagc catccagaat
    13321 acccgtcacg gcaaaggcat tgtacgcatt gatccagaac gccaaacgct cccccgccga
    13381 caagcggccc ggatccactc gattcaacaa cgagacatag gacggaagtt gcccctggcg
    13441 gcgaatggcc gggtagtcaa ctacgccgtc acggacatgc gtacccagta gttcgtgcca
    13501 tacccgatgg gaaagctgct ccggcgggag cgggtctgag ggggtgaacg aggtcggtac
    13561 ggccgaacag cctcccagga caatcatcag cgctccgatc agaaattttc gcatgacaat
    13621 ctttctccta ttggaatcgc accggagtcc gccatgccga gagcggatgc ctcaggaccg
    13681 tcccgaccac gcgcccatcg agacggttgc aggaggagca gtcggaacag gcttgaatct
    13741 ggggcccgct gaaacgactc aggatctctc gataaggcaa ctcccgaagg ttccccaccg
    13801 aagacatcgc caggcaggga gaaacctccc cggaggcatc gatcgcaatg ctataccgtc
    13861 cggcatcgca aggctcaagt tgttgccctc gcaaccatcc ggcgctgtcg gcgacgaatt
    13921 ttcggaagta cccgggaggg atcagatctt ccttgagaag caactcgaac accccggcgg
    13981 cggcggagcg gtcgtacatc aacccggtgt cctctttgcc gtagaggccc acgttccagt
    14041 ggtaggctcc cacgacggga ataaagcccc gagccttggc gaatcgcacg acgtcgggca
    14101 cctcttcgcg attgacctcg ctcacgatac aggtaaggaa cttggggtag ggatactgtg
    14161 ccagaagact aagtccagcc aggacctgtg gtaactgatc agccccgcga atctgtcggt
    14221 agcgcgtacg atcgagcgtg tccaggctga ctgatagagg caggcgcagg gaggtaaacc
    14281 gtgcgacgag gtcggttgtg agcctagtcc cattggtgat caccgtcaca tgcaacccca
    14341 gcgaagacag gtcctccagg atgtcgacaa gatccggacg cagaagcggc tcgcccccct
    14401 gaacaagcac gaacctgata ccttcggtat agagaaggcc aaagatgccc cggatctcct
    14461 ctcgcgtcag ttcatatcgt ccctggttca gaggaagatc gcagtagcca caggcggagt
    14521 tgcaacgaag ggtcacctga aacgtggcca ggaccggccg ccctcgcacg agattagcca
    14581 acggccgggc acagtcggca atcatggcca gcgaaggctt cctcgtcatc gtggattccc
    14641 cggtcggcct gagcatattt gaacacctgt tgccatttcc atccggcccc gccgcccccg
    14701 gttcagcaca acgcgacaca atcggcacca atccaggcga ttctgcactg caggcaaccg
    14761 gtttcctggc atacggcttg ctgaatcact ctctacactg cagcctgact catcccaggg
    14821 aggatcgtta tgaacaccaa gctccaggcc tcgatactca ccatcgccgc ggctaccctt
    14881 gcaaccgtcg cttttgtcgt ggctccggca tcggcaaagg acaaggagaa ggccggcaaa
    14941 cattcgaacc atgcgaccac cacctcggca gccgcggcct cctcacttct gcgatcagtc
    15001 cctgagcaac ccccggcacc gaaacccgga caacatgact ccaaacccgg agtcgcctgg
    15061 aagaccgtgg gcggcaccgt caaagaaatt cacggcgaga cgtacacagt cgaagacttc
    15121 gacggcagcc aaatgaaggt gcatgtcgga cagggaacca agcacctccg gggcaacaaa
    15181 aaagtgggcg acaccattcg ggctgaaatt acccacggtg gcttcgcaaa ttcaattcaa
    15241 taaccccctg actctgcgaa cccacatcag ctcgccggtg tcctggagag gccgaccctg
    15301 cacaggtcgg cctccccgcc tttctcagtt ccggcagttc cccagccccg tcacttcaac
    15361 gccctgacga agacgatgtc accgtaatag gccacggccc gctccttggt gttatccgta
    15421 tcgctcataa tcgcgacgcc gctgatcgcc ggcggctctt cgccgaaggc cagtcgataa
    15481 tcctcataca cattgcgctc ttcctcgacc cacgtgccga tgcgctgcgg cccgctctcc
    15541 accacgatca tcttcgcaaa gtcggtaaag gcattgtcca ccaccgttcc gaccaggctc
    15601 tggccgtccc aaatgtaatt caatgcgccg atcggaatat caccgaacag cacccgaccg
    15661 gcctggtact tcaatttccg tccgaaactc acctttgccg gttcataggc aaaggtaata
    15721 tagaggcgcg cgggatagtc atcaccgtcc ttgcggttca cattgccctt ttgcagcaga
    15781 ttctccacct tccatcgcca gcgcacgatc ggatactcat gcgggtcaat cgccacggcc
    15841 ttggtcaggc ctgaggcgga cgcctcgctc acagctttca ccacggaaac cggcccgtcc
    15901 ttcaccatct cgtatgtcgt gtgccgttcg atcttcttga acgtcagcgg tgcccaccca
    15961 tcgggcaata cggcgccttc agccgcgacc gaaaagtgtc cgacctccag cttcgcagag
    16021 ccctgcgcat gtgcgaccat cgcagcaccc gacaagcaca tgagcaggac aatcccgcgc
    16081 cgcatcatgt gtcccctcat cgcctcaacc aggcaaagag cctcgttaac agcatcttag
    16141 tccgcgccgt aaagtgcgcc ttgcgccaga ggttgacgac cttcttgttg acttccgccc
    16201 gggtgggata aggatggatc gtggcggcga tcgttttcgc accggcaccg gccttcatcg
    16261 ccacagaaaa ctcactgatc aagtcaccgg catgcgccgc cacgatcgtg gccccgagaa
    16321 ttttgtcggt gcctttctga atatggatgc gggcaaaccc ttcttgctca ccatccagga
    16381 tggctcgatc cacctcaccg agtttgtagg tataggtttc gatctccaag cctttctttt
    16441 tggcatccgc ttcgtagagg ccgacatgag cgatctcggg ctcggtaaac gtgcaccagg
    16501 gcatgatcag cgactccatg ctggcatacc ccagcccgaa gggatgcggg aacaatgcat
    16561 tttgaatgac gatctgcgcc atcgcatccg cggcatgggt gaatttatgc cgggaacaaa
    16621 tgtcgccggc ggcgtagata cgcgggttcg ttgtctggag gcgatcgttc actttcaggc
    16681 cggttttgtc gtactcaacc ccgatcgtct ccaaccccag cccctcgaca ttcgggctac
    16741 gcccgacgcc aaccagaatt tcgtccaccg tgacatcgta gtgctggccg tgggaatcaa
    16801 ccagcagccg cttcccggtg gccgttcgtt ctactctcag atccttcccg caacaaagta
    16861 tcctgacacc gtcccgcgtc atggactgct gcaccacctc cgctgcgtcc cgatcctcat
    16921 tcggcatgat gccgtgcatc gcttcaatga gtgacacctc gctcccgaag cgagcgaagg
    16981 cctgcgccag ttcgcacccg atcgggcccg cgccgatcac agcgagacgc gccggcaact
    17041 ccgtcagcga aaacaccgtt tcattggtga gatatcccac ctctgccaac cccgggatgg
    17101 ccggcgccga agcccttgcc ccggtacaga ctgccgcgtt gacaaaggtc agcgtccgat
    17161 ttccagatgg gccctccacc gacactgcat cccagccggt gaagcgagcc tgcccgatat
    17221 agacatcgac ccccagcgac ttgtagcgat gagcggaatc cacatggctg atcccggcac
    17281 gcaattttcg catcctggtc attaccgcac cgaagtcacg agtcaccccc ggcgggatat
    17341 gcagcccgaa agccgaggca tcccgcaact gcgcccaagc attggccgca cgaatcagcg
    17401 ccttggaggg cacgcatccg acgttcagac aatcgcctcc catcagatgg cgttcgatca
    17461 acgcgacctt tgcgcccaat cccgccgcca ccaccgccgt gatcagcccc gcggtgccgg
    17521 caccgaccac aaccatgttg tagcgaccgg caggctcggg attcagccag tccggcggat
    17581 gcacatttgc aaccaacgtc cggttgtgtt cgtcatccgg caggaccaag ctttggacag
    17641 aagcgctcat tgaagtcgga ttctccctct ggtgaataca catggccgcg gccgtgcagt
    17701 tagaccgcct taccggcaaa cttctgatag agaatgggca ccagcgccaa cagaccgagt
    17761 aacgtgaacg cgagcaacac attgggcgag gcgatttcct tgagtgaact gatcgtgccg
    17821 agttggcggc cggcatacgc aaagacaaaa ctgccgggaa tgatgcccaa ggaggtcgcc
    17881 gccatgtagg tccccacatt gaccctggtc agccccgaga ccatattgac cagaaaaaac
    17941 gggaacagcg ggatcaaccg cagcgtcatc agataactga acgcattcct ggcaaatccc
    18001 tgctgaatcg catccaaccg gctgccgaat ttctgctcca cccattccct gagcagatag
    18061 cgagccacca aaaacgccag cgtcgcaccc acggtcgcac cgacattgac gtagagcgtg
    18121 cccaacaggc tgccgaagag aaatcctccg gccaacgtca tgatcgctcc accgggcagc
    18181 gagagcccga ccaccacaca gtagccaagc acgaacatgg ccaccgcgac cggataattg
    18241 gcttctgtaa acgcgagcag acgagcccgg ttggacttga gcccatccag cgagaggtac
    18301 tgtccgagat caaagtagaa aaatgcggcg aggctcagcc cgacaaaaat ccccagcatc
    18361 agtttgccga gcccggcgcg tttcgaagat gagggcgatg ctacagatgt ggtcatggca
    18421 ccttgggggg gtatcatcct gcctcctcac tcatgtgtca atcgtggact cgcctgcaca
    18481 gacaatacac cgctcgtgac tgaaagaatg tcagcaacct tacagaggga ggagcctgct
    18541 tgtcaccaca ctgcaccgta tacggagagc gaatcagaat agattgccca cctacacgat
    18601 gatgaagcac tatggaatgg gcacgtcgat caccccttgc ctttactttc agaccatttg
    18661 agtgacgctc tgataaaacg cttctctctt ccagggccag tggacctcaa gtacccgtct
    18721 tgtccctccg aaagaactct tgaagacagg gtgccagtgg aggtaatgtc tcagatgcat
    18781 gcgctgattg aactcttgga ctcggctatg ctgacaatac actcggtctc acagcagtcc
    18841 cagaactctc accccgtcta tgagacgact tcacatgtgt ggatgcgccc ctgccctcac
    18901 tgctcctcgc gcctgagaaa gaaatcacct gaagaatcat ggcgttgcgg atgcggttgg
    18961 cacgcagagt gaagtggtac aattagatca ttgtaagggg gtgacgtcat gggaccgctc
    19021 tcggtgcacg acatgaaaag tggatttttc ctcttcgcaa gcttcctgct cctggcaacg
    19081 atctgcgcca tcgcggcctt gggcacgttg atgcgtccgc agcgctggtc tgaacgagag
    19141 accagactgc acgtctgatc ctcgccaaga gaaccaacgg ccaggctata cgcaggccgg
    19201 gggtgtacct gtgccgtccg atcactcgca tcactttctc ctgactatcc gcctcgtcac
    19261 gctcactccg aaggccgaac tccccgacaa tactggactt cttcatttct tagcggacag
    19321 gtcacggagc acccgccacc cgatcgaaag taccggcctt gtaagatcct ggcctcttgc
    19381 cctactctgt ctccaggagg ctgggtatgg atcagaatga ccgtttcatc aagaccaagg
    19441 aaaattgggc caaggcccga cgcggcgggg aagaacgcga ggtcttttac gaaggagatg
    19501 atcggctccc tccaggccaa catctcgtgg aaacctggcc ggtactcgac ctgggccaaa
    19561 agcccgatat tcccctgtcg gaatggacgt tgaccatcgg aggtgcggtg accacgcctg
    19621 ccacttggac ctgggcggac ttccttgcgc aaccgcaatt caagaacgta tcggacttcc
    19681 attgcgtgac gtcctggagt cgttacgaca atgagtggga aggggtcagc ttcaaacagc
    19741 tgatggctgt cgtccagccg ctcccgtcgg ccaaattcat cctcttcaaa tcgtacgacg
    19801 actacaccac gaatttaccg ctcgtcgcct gtcaggacga cgacgtgctc cttacgtaca
    19861 aatggaacgg ccggccgctc accaaagaac acggcggtcc ggttcgcatg atcatcccga
    19921 aacggtatgc ctggaaaggc gccaagtgga tcaaggaaat caccttctcc gaccacgacg
    19981 agaaggggtt ctgggaagtg cggggctatt cgaacagcgc cttcccctgg cagaatgacc
    20041 ggtacggatg aacgaacaca attctccggt gaacgcaccc atcatacagc gtatcgaaat
    20101 ctggccggtc gatattccga tcacggatcc gttcgtggtc gccacggggg cacgggtgac
    20161 ggcgcaaaac ctgttcatgc gagtcacctt gcggaatgga actcagggtg tgggagaagc
    20221 cgcgcccttt cccgaggtcg gaggtgaaga tcgggcaacc tgcttcgccg cagccacgga
    20281 actggcgaag actatgatcg gccaatcggt cggggactac gagtccctgg cagaccggct
    20341 caacgaacag gccacactcc accctgccgc tcgttgcggc ttggaaacgg cgatgctcga
    20401 cgcctattgt cgatcacggc gcatcccgct ctggcaattg tggggagccg cggacgtccg
    20461 cgagcgggaa accgacatca ccatcccgat ctgcagtccg gaaaagacgc tggaactcgc
    20521 acgtggctgg tacgccaggg gattccgcct ctttaagatg aaagtcggga cggatgtgga
    20581 acaggacatt cgccgattac aggccgtgca caacgcgctc cccgagatcg gatttatcgg
    20641 cgacgggaat cagggattct cccgtgagga ctgtctccgc tttgtccatg gagtgaagca
    20701 gttcggcggg cggctggtct tgctcgagca accggtcgtg cgcgatgatc tcgaaggact
    20761 acaggcgatt cggcacctga cgggcattcc cgtggcggct gatgagtccg tccggtcgct
    20821 ggacgacgcg cgcgaggtcg tccggatgca ggccgccgac tatatcaaca tcaagatcat
    20881 gaaaaccggc gtgatagacg cgtggcgaat cgccgccttc acccgctccg ccggcctgcg
    20941 tctcatggtc ggcggcatgc tggaaacccg tatcgccatg ggctgctcat tcagtctcgt
    21001 gctgggcctc ggcggcttcg atgtgctgga tctggacaca ccgctcctcc tctcaaccga
    21061 cccggtgacc ggagggtatc gatatagcgg cccgcgtctc cagccctggt ctgaaggcgg
    21121 actcgatatg cagatccctt gtcccgcaga ccgcatcacc gtccagtagc acggatccgg
    21181 acctcctcac gcaacctgtt tgctctctga ttctcacccc cgacaacgca ctgcaggttt
    21241 cccgaaatat tttgccgttt ttccactttt ctgaaaaatt aacctcaggc acagcggctt
    21301 cgcacaggcg gagcaccgat cgcaacgact ggcaccgtac gtacccggga ggaaggtccc
    21361 atgcaagtaa tcactccgcg cccgatactc gcgaccgcgt ctcctcgcaa catccgtccc
    21421 atcgcactct tctggatgct tggagtcggt accttgatgc tgggaggctg tgtttcgcaa
    21481 caaacctatg agacagccag gcatgaggta aaaacgcggg ccactgaact cgcccagact
    21541 caggcggaca tccagagcct cgaacaacaa cgagaagaga cccacctcgc caatcagcgt
    21601 gaggaacgct ctcttgccac tctcaagggc gaactgaaaa agatccagct cgcctacgag
    21661 cagcttcaca agagcaacca ggccaaactc gcggccctcg agtacagcat cgtggcatta
    21721 cgcgcccggc atcaggccat gttgaaggaa atcagcgaga ccaaacggat cgagaaacgg
    21781 ctggaagcgc tcacggccca acacgaacac acgatggcga caacaccatc cggcccggag
    21841 gcccgtgtga ccacggtgga cggaagcccg caggagccgc acctggtcgc ggtcatcacc
    21901 ccgcaaacgc cacagattga ttccacgccg accgcatcgc aggcgccccc agccgccacc
    21961 agtgccgcca cgacaccggc ggtgcctcag cccgcaccgg tcgtcgcaac cccgccttca
    22021 cccagtactc ctgcaaaggc cgcgcaggca cccgctgcgc cacaagcccc gcgacccgcg
    22081 accgcagcag cccctcagga cgaatcctgg ttctcaagtg tgacgggatg gttcacgtcc
    22141 ctcttcgact ggatctgggc ctagagatcg tcctcaacgc ctctctccat caattctgat
    22201 ccaggcgttt catcgcctta tcagcccacc ggagccctca agactgggcc ccggtggggt
    22261 cgaacccgtc gcccccttct tcacactttt tacaattcaa ctccgtcccg aggaacgctc
    22321 gccgcccctc ctccgtcaat acccacggac gactcgtcat aggcggctga tgtcgcacat
    22381 gttggccatg accgcagcga aggtcggcca cccaatggcc ttcgtcgtcc tggtgatacc
    22441 cgataatcgc ctgttgcata ccggactccc cgtgtttcgt gccgctgttt tgaattgagc
    22501 actcacctga cagaaccgga cgcgtggata cctgcatcca aagggactgt ggtagacttc
    22561 cgcgcccggc agagtagcat gttttccagg aggttgcgga tggcacagtc agaatttcga
    22621 atcggcatcg tgggaatagg acggatgggg gccaacatgg cccggcggct acacgacctc
    22681 cactatgcga tcgtggcggt ctatgacacc gacacggaac gcgcgggggc gctcgcgaag
    22741 gaactcgcgt gcacggcggt cacgactccg gcgcgtgtgg ctgagttggc cgatacggtg
    22801 ctgacggtgg tgtcggacga tgccgccatg cgccaaatct attcactcgg cgggccagac
    22861 agtttgctac gccacgccgc caatcgcctg ttcatcaatt gcgccaccct gtcacctgag
    22921 ctccaaagag aagtccatgc gttggtcgag caacagggcg gccgaatcct ggaagcctgc
    22981 atggccagca gcatcacaca agcgcgccag ggcacgctct atttaatgtg cggcggacga
    23041 ccggaggtgt tcgagcgggc gaagccgctc ttgcaggatc tgagcgcaca cctgcggtat
    23101 atcgggcctg cgggcgaagc cgccaaggtg aaggccctgg taaacatggt catgaacgcc
    23161 aacaccgccg cgctggctga aggactcgga ctggggtcgg ccctcggcct ggacctcacg
    23221 atgcttcgag aagtcttttc acagaccggc gccaactccc gcgtgctcga aaccgacggt
    23281 gaggacatgc aacagcgggc tcacgactgc tacttctcag ccgcccacgc ggcaaaagac
    23341 tcggggatcg cctgcgcatt ggcccaacag gcaggcttgg acctgcccct ggccaaagcc
    23401 acctacgatc aatatcgacg tctcatcgac catggtaagg gagaattaga taaatctgcc
    23461 gtcgctgagc tgaccttcaa agaccggctg aatcattgac gatctgacgc cccacacatt
    23521 ctaccgatgg aagcagcgcc ccggtcacag ccgcaccgac caccctcgca gtagttcacg
    23581 aagcccgcgc tcgccggtcc gctccgggct aatgtttttc gaacgaccgc tccggtcgca
    23641 cggacgcatc aagaaatcgc caccgcttct gggcatcgac atcgaacgcg ccaagaaatg
    23701 tggcttgacc gaccagcgat tgaaactcca ccaggcgaag gctgcagttg gccggatcga
    23761 acacccgcgc atcgtactgc cccaagaccg tgatgacgcc cgacggctgt tggtagacat
    23821 tccgccgccc ggcatacccc gtgtcgggaa acaattccgc cgtgactgag cagccgcgcg
    23881 acccttcaat gcgaagggtc aatcgatagc gggagagaaa agggtgcgtg gcgaggcgag
    23941 tcaggttcag ccgaagcccg gccgagggaa gatcgaactc ggccggctca gggcgatcac
    24001 tattgcaggc cgacaggagg aggaccagac cgatcagagc ccagcccggt cctctccctc
    24061 gtcccctcat catttggccg taggcatctt cgaggccgct ccgattgcat caaggcccgc
    24121 ttcggcgcag gcatcgtcca gatgcgtacc aggcgcgccc ccgacaccga tcgccccgac
    24181 cacctcgccg gcgatttcga tgggtaaccc gccaccgacc atcagaatac tctcattcat
    24241 ctctcgcaat gcttgcaatg cgggctgctt ggcaatcatg tcggccaagt cgctcgtcgc
    24301 gcgccgcaag ctggcagcgg tataggcctt ctttctgctg ctatcgacgg tatgtggacc
    24361 agccccgtcg gcccgcatca tggcccgcaa gacccctgcg cgatctacga ccgacgcact
    24421 gacccgatag ccgtccttct tgcagaaatc gagcgccgct tgcgcagctt tacccgctaa
    24481 tgccgccggc aacacagcct ctttgggtaa ttcatccgcc gccgacgcca ccacaggcca
    24541 cccgccggcc aggaacagaa cccccgcaat catgccgaca cgccccatgc cccgtttcaa
    24601 caattgtatc gtccccacca tcacacgcct ccttcgttct atatcccctg cgtagtcaac
    24661 cgaacacccc tatctgtatt ccgacgtgcg agggtacggc catcaaaccg ctctgtcaat
    24721 actcagcgct caagctttca gatatgcgaa caggtaccgg cgtgctagga atacagggcg
    24781 aacttcgcgg caggggacaa ctgttcgtgg ccgaccgatc gagggaggga ataacgggcc
    24841 gcatcgagtc cggcattgga ggtgtagaca cccaccagga cgattcgcgg atgcgtaagc
    24901 gaccgacctc ctcggtgcag cccactcgca tcacacagga tgacactgcc cgccggcccg
    24961 acgcaggaga cagacggcaa cggcgtatcg ttgatgagtt ggtcgagcac atcctgtttc
    25021 gggtaccgac cggtcggggg aatggcagga aagagcgatc cgtactcacc gcccacctga
    25081 gaacgcttca cataagtgaa cggccccatc gtcccatcga catcgctcaa caatacaaag
    25141 accttgatga tgcgcttatc ttcatggtcg cgatgccaga gctccgcaga gatcggcggc
    25201 tcaccctcac acaccgggat gttatgccag acgtccatgt agttgagacg gacctgcagc
    25261 ccgagataag tactggcgat actcagcaac tgcggatgga gaaagaaccg gatgaaagac
    25321 gagggaatgg gcggtcgttt cgcgcgacgt ccgatcgccc gcaccacgta ccatttcgca
    25381 tcgttcggat tgtatcgctg cctgatcttc cgttggacat cctgcccggc gagcatcatc
    25441 ccggcatccc gagctagatc ccggaacaaa gccggatctt gcgccagagc ttcgacggag
    25501 gtgcgaaaga tcccgtcggt ttcgagagcg cgaaccgtct cgacaccatg cggaccaagc
    25561 acctgcggaa tcgagcggaa cacccgccgg gatcggcgat tgatcgcccc ataaagaggc
    25621 ggggaccagc gaagtcgata gaagagccgc ccgaggatcg acaagggcca gacgtagacc
    25681 atcacaccgc gcaccagctg caggcaggtt tttccacgcc acagtgtgtg ccaccctccg
    25741 actcgtttca cgccaccccc gcttgccgct caagcacgga caacaagaag gcttccggtc
    25801 gtctgcgaca ggagaccggt tttccagcat ccccctgcag ctccgggaca tcggggctca
    25861 taagagatcc gagccgcaat ggcctgcgca tcagacaacc actccgtgat gattgaggga
    25921 ctcagttcaa cactgctgac tgtgtgagaa gcgggcgagg tcaacagcca gctagcaggt
    25981 tgtcggttca gcgccgccgc tcttgtattt ttttgaatcg gctgcgatca ggtattccag
    26041 gcgcaccgcc cgcaggcggt tcaaaaaggc cgtccagcaa ggccgtagcg aatgaagggc
    26101 cgaggcgtac cctctgcggt acgttgagac tctgaatgga gcgagaacgc cggcggcgga
    26161 ctttttcaac ggcctgctag aaagtggaga tgggatggat gacagcatcc agcgtgccgg
    26221 gacgatgcaa ctcctcgatc cagacccttg cttgcgcgac gtaaaaggta tagtcgctct
    26281 ccgggtaact cagcaccacg gcgtagagca gctgctcggc ttcctgctca cgtccggcgc
    26341 tcaggctcgt ccgggccgcc tgcaaggtgc acgaagcggc cagcgctttg ggatcaacag
    26401 tcgtgcgaac cggctgtcgc gtgacaaacc ggtcaagact cttgggaaca tccggagcgg
    26461 gaaccacatg cgtatcagcg gactgctgca gctgtttcgc tgccgacaag accgtctcga
    26521 catcgccgct cgattggcaa tgccggtaca attcccacat cgacatcacg gaagccgagc
    26581 gaggtgccgg ctgacgcgtc gaggaatcga agagctgaca gccggtcagg aataacccgg
    26641 cggacaggag tgcgacaatt gtgacggatg tgtgctgacg catcttcctt atccttaccg
    26701 gatcgcttct ttggctgtca agatttccgg agcagaatct ggaaggaacg tgacaagaca
    26761 ggagcactca cgcgctcatg tcagcatgga accggcaatt gatgaaggaa ccgcaacgtc
    26821 acaattcagc gcgggctcga tgatcgctca tgaaccgcgc cgagatcttc aatgagcgcg
    26881 cgcaagggtt cggccacgga ccaggcctgc gccacgcagc cacgcgctcg atgcgggtgt
    26941 tgaccgtcga agatctccga cacgtggccc aggcaggctt cgtgtaaatg ttcgtgcaac
    27001 ccgtcgagaa acatgcgcgc atcccgccgc accgccggac tatcgccata ggtgttcacc
    27061 cacgctgtca cgaacgggcc gagcagaaac ggccatacgg tgccttgatg gtacgcgcca
    27121 tcccgctcgg agaccccgcc ctcgtacgaa gcacagaacc gaatgtcctc cggtgccaac
    27181 gtgcgtaagc ccagcggcgt gagcaatcgc tctctcacga gctgcaagat gtgtttagcc
    27241 tgcgcctccg tcaccagctg atcatccaac gccaaggcat aaatctggtt cggacggagc
    27301 gaggcatcgt ctcctatcgg cccgtccacc acatcgaaga gatagccccc ggtccggtac
    27361 cagaagcggg ctcgaaacga ctcggtggcc ctcgcacgat cctgccggca ttgtgccgag
    27421 tacgccggtt ccccgaattg caccgccagt tcggcagcca ccgccaatgc ccgcacccag
    27481 agcgcttgaa tctcgaccgg tttcccgtgc cggggagtca ccacccaatc gccgattttg
    27541 acatccatcc aggtgagttg aacgccctcc gcgccgccgg taatcatccc gtcgtcatcc
    27601 atgtgaatgc cgaaccgcgt gcctcgacga tacccctcca caatctgttt gatcgcgggc
    27661 caggcgatcc ggcgtactcc gtcgaggtcg cggctatagt gaagatagcg gtccacggca
    27721 tgaataaacc ataacgacgc atcgatcgtg ttgtactcag gctgttcgcc gatgtcggga
    27781 aatcgattcg gcaccatccc ttgcgacaca tgagacgcaa aggcctcgat cacctgccgg
    27841 gccacgtcgt aacgacccgt caccaggcag agcccgggca aggcgataaa tgtgtctcgt
    27901 ccccaatcgg caaaccaagg atacccggcg atcaccgtct gcccgtttcc ccgccggaca
    27961 agatacgcgc cgctcgcaac ccagagttcc tgcgccaacg catcgtcggt cggcgccgat
    28021 accggacact ctgcgcgccg ccgccgctcc gccgctacaa cctgcgcgac atcgacggac
    28081 tcaagctgtt cggtcgtgaa cagaagttcc gccaccgatc ccggagtgag cgtgaactca
    28141 cattcgcccg gcgaccacca gtcctccgta tggtccaggc cgcgttcgcg ctcgatccga
    28201 tagtgaatgt ggcggtacca atccggccca tggtgatact ccccgttgtg gaacgccagc
    28261 acggcaggta gcgcatcgta gggttgccac gtgacacggc cctcgtgaac cgtggcctga
    28321 ctgcgcaccc cggcgttctc ctgatgcagc gcatggaagt cgcgacccga gagcatcggt
    28381 ctgacccgca gcaccacagc agacggtgcg tcgccaagca accgccatcg cacgacgacc
    28441 atatcgcggc catccggaca gagcagttca cgctccaagc ggattccatg ggaggcgtaa
    28501 cgccaggtcg gccatggcag ggcggagaac ccatcgcaat accgataccc ttccggatga
    28561 accgcgccgc tatacagatt tgtcgagagc ggaaaggatt ggccgccgac ttccaccgat
    28621 tcttcgagat gattcaccag gaccacacga tcgaccggcg ggcgtctggc caccagcagc
    28681 aacgcatggt agcgtcgcgt attcgcgccg gcgacggtgc ccgaagcata cccgccccgc
    28741 ccgttcgtct ccagccattc cagactgagt gcccggtcaa ggtcctgaca gcgtctcgaa
    28801 tcgatggtca tcccgtgcct ccggcttact cgcccgactg ctgaatcaac ttggccacca
    28861 gcccggtcca gcctgtttga tggctcgcgc cgatcccggc gccgttgtcg ccatggaaat
    28921 attcatagaa caagatgaga tcgcgccagt gcggatcctg ctgaaacttt tccgcaccgc
    28981 cgtagacggg acgccgacca tcgggcccgc gcagaaaggt atgcgtgagc cgccgcgaca
    29041 gctcagccgc aacgtcggcg agagtgcgct tctgtcccga ccccgtcggg tactcgaccg
    29101 tgaagccgtc gcccaaataa tagtgaaact tctgaagaga ttcgatcagg aggtagttca
    29161 cgggaaacca gaccggcccc cgccaattgg aatttccccc gaagagaccg ttggtggatt
    29221 ccgccggttg atattcgacg ctttggctcg tgcccatcat cgagaacacg tagggatggt
    29281 ccttgtgata acgggacaga gcccgcacgc catacggcga caaaaactcc tgctcatcca
    29341 acatgtaccg caggaccttg ggcagccggt gacgattgac gagcgataag aaccggcgca
    29401 cctccccgtc ctgcgactgg gtttcaatgt gcagactgaa atccggccgg ttttcgatga
    29461 accattgcat ccggtgctta aaacggggca accggtcgat gagctcggaa tccagcgtct
    29521 ccaccgcaaa caacggaatc aagccgacca tcgaccgcac cttcatcgga aacttcttcc
    29581 cgtccggcag gtgcagcacg tcgtagaaga agccgtcggc cttgttccag agttcgatct
    29641 tcgcatcgcc gatgttgttc atcgcccggc agatgtagac aaaatgctcg aagaacttgc
    29701 tggcgacatc ttcataggcc cggtcttccc gggccagttc cagcgcgatg gataacatgt
    29761 tcaggcaata cattcccatc cagctggtcg cgtcggattg ctcgatgtgc ccgccggtcg
    29821 gcagcggctt gctgcggtcg aatacgccga tgttgtccag ccccagaaat ccgccctgaa
    29881 aaatattctt cccctcggca tctttccgat tcacccacca ggtgaaattc agcaggagct
    29941 tgtgaaacac ccgctccaga aaaatgcggt cgccgacgcc tttccgcttc ctctcgatct
    30001 tgtacacccg ccaggccgcc caggcatgca ccggcggatt cacgtcgccc agggcccatt
    30061 cataggcagg aatctggccg ttcggatgca tgtaccattc acgcagcatg aggacgagct
    30121 gatccttggc gaaacgcgaa tcgacgagcg cgatcggcag gcagtgaaac gctaagtccc
    30181 aggccgcata ccagggatat tcccacttat ccggcatgga aatgacgtcc gcattgtaga
    30241 gatgtgccca atccgcattc ctgccatgca gccgctcctt ggggggttcc ggtcccgccg
    30301 ggtcgccctt cagccaacga tcgacgtcgt agtgataaaa ctgcttgctc cacaagagcc
    30361 cggcaaaggc ttgccgctgc accattcgcg catcttctga cacgttcgcg ggggccaacg
    30421 cggcataaaa ctcgtccgct tccttgagac gggcggcaaa gaccgcgtcg aatgcctgcg
    30481 gatcgagagc cccttcggcg gccccgttcg tgaaccgcaa tcgaatcgtt acaggccgat
    30541 caggcaacgc ggtcaccaca tagtgcgcag acgccttcga tccgatctgg tcgggattga
    30601 ccgcgccctt gtcgccctgc acgacgtagt cattgatccc gtctttcacc tgccgtgcgc
    30661 catccacatc gccgtagaga cgccgggtat tggtttcgtt ttcggtgaac aacagatccg
    30721 gcccaccctc gcagagcaac cgtcgcggcc cgtaatactc atggtcgaac tccacaatac
    30781 tgaccgacga cgtcgattcg cctttccgca tccgcgggcg gcgcacatcg agtccccacg
    30841 accaggtgtt gcggaaccag atggtcggca gcactgtcag ctgcgccgga tccggaccgc
    30901 ggttgacgat ctggatccgc atgaggagat cttccggcga ggccttggca tactcgacgc
    30961 ccacgtcgaa gtagcggtcg tcatcgaata ctcccgtatc gagcaactcg aattccggat
    31021 cagtccggcc tcgccgccgg ttttcttcga tgagtcgcgc atacggaaac gccgcctgcg
    31081 gatacttata gagaaacttc atgtaggaat gcgtaggcgt ggagtcgaga tagaaataat
    31141 attccttggg atcctccccg tgattgccct cgttccccgt gaggccgaac atccgttctt
    31201 tgagaatcgg gtcccgtccg ttccagagcg ccagtgcgag acagacgaac tggtgccgat
    31261 cacaaatgcc ggccaatccg tcttcattcc aacggtaggc tttcgagcgg gcatgatcgt
    31321 ggggaaccga ttcccaggcc gtcccgtacg agctatagtc ttcgcggacc gttccccaag
    31381 cccgttcgct gacatagggc ccccagcgtt tccagtgttt cctgcgcaga tcgtcctcat
    31441 ccaggcgctg ctgctcagcg gaccccggac gaacagcctc ctctcccggc gatgtcgaca
    31501 tagcacgagg tctcctacca gtgtgttgac gtactacata ctccgagcag aagtgcggaa
    31561 accagcagag ccatagacgt tcccagaatc cgctacaggc tgttgagaaa ggcggttcag
    31621 caaggtcgca gcaggggcca gcatgcgccg ggctcgaaaa agattcaacc gagattcgag
    31681 ggcgtcagga taggaaataa gtcagcttcc accgcgcacg acaggagtcg agtcagcaaa
    31741 ccgatctttg cgaggtccgg cgaaagaaac tatgggtggt gacaggggac tgggaaggaa
    31801 cactcagcgg gaagacacag gccctagctc caaacaacgc cttcttggtt catgaggtag
    31861 tcgatgactt cgatgctgtc ccgtaactga agttgcgcct gctcggacat cggcagtatc
    31921 gccccaagca gtttgccgga ttccagttcc gccaggttgg gaatcccgtc ggtactccgc
    31981 ttttccaggt cgaatctgaa actggccact tgttacctcc aatcccttgt cggtcgtagt
    32041 gcagcaattc ttgagccaca ccggagaaac ctggatgcca tgcgtacact gaagcatagc
    32101 acaagggctc tttcgactgg atcagagggc taacctctta atttgttatc gatttactga
    32161 cgacag
//
LOCUS       DAORRQ010000048        32093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_85_length_32093_cov_22.065547, whole genome shotgun sequence.
ACCESSION   DAORRQ010000048 DAORRQ010000000
VERSION     DAORRQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 32093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32093
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85_length_32093_cov_22.065547"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2140)
                     /locus_tag="PK224_14565"
     CDS             complement(<1..2140)
                     /locus_tag="PK224_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB17015.1"
                     /translation="MHHLRSFQLRDMTACGAALRRLGTDATSLEQVADRLVRHLYGSL
                     TMGHLREPACSLVRLFKTTPYSRLTPDLRALADARLGDTPPPPSLTCLTLLASAGAVP
                     GWNDPARSSRFRVIPLDTLEAVERLPMFSQLFRQLGVSLPSLTQPGPSVLLDQHEQSF
                     NVFHIPEAEGSPYVPGQEEFVLKYGIRSVLGFGAPLPDGELFSIILFSKDFIPESTAT
                     LFKPLALCAQIALAPYATTTAAFHPHHTSKPEQAGGDPTPVHDAHLQARIADLERLLA
                     VHEQTVDEQADRMELIVQGSQMGTWDWEIPTGRVTFNERWASMLGYRLDELEPHVRTW
                     EQLVHPDDLHQVMATVSSHLRGETPTYSSEHRLRTKSGAWCWVFDSGRVVKRDAKGAP
                     LRAAGIHLDISDRKALEAAQARAQCDLQAKQQALDEAQTLAHLGSWHWNIATGQEAWS
                     EEQCRIFGHTPDQSLPTYATFLSALHPDDVERVQQAVDAALKYDSPYNVDCRIRRPDG
                     EVRHVNCRGVVQRDPDGRPVSMAGTVLDITDYKRAEFAWRDSETRMRSIFESAIEGIV
                     VIDERGRIESANTALLNLLGYQAHELVGQNISLLMPSPYREHHDEYLANYLVTGKRTI
                     IGSGRDLPAVRKDGTRIDVHVSVSEMQIGTEKKYTGMLRDISERKRMEATLRESEERF
                     RQLAEHIDAVFWLTSPDKSEVLYVSPAFETI"
     gene            complement(2245..2901)
                     /locus_tag="PK224_14570"
     CDS             complement(2245..2901)
                     /locus_tag="PK224_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17016.1"
                     /translation="MNEIQNTPARVFIINPQELIRVGMRTLLNSVPDFMVVGEASSKE
                     DALPLVLHHKPDIVVLDLRVQDGTGIETAKDILAHLPETRVLFLADALTDSTLLSAVA
                     TGAHGYVLQDAGVDTLLHAMRSLTKGQSYLDPGVTRHTFTYLRKVAEREPERGRHLLS
                     PQERRLLPLIAQGKTNKEIAAELGLSDKTVKNYLANVYSKLHLSRRSQAAAFYLKTIP
                     "
     gene            complement(3147..4472)
                     /locus_tag="PK224_14575"
     CDS             complement(3147..4472)
                     /locus_tag="PK224_14575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRB17017.1"
                     /translation="MTPQTSRRVVITGMGVISPLGCTVDLFWHLLSKGESAVKPVSSF
                     DTSPFQACLAAEVRDFDPEDFLHRKQARRMGRATQFAVASAMMAARDSGVELEQEDRG
                     SIGISIGTSIGGMKEAFEFHDAAKLNAYERVNPFTMGMTFPNAISSEVAIVLGLHGPC
                     ETYSIGCSSTANAIGRAYEWIKSGQSSLVVAGGTEAPLHPSVYAAMNAGRALAPDERG
                     AIRNLPRPFDKTRCGMVLGEGAGCLILEDYEHARARGAKMYAELEGWGFTCDAHSMVK
                     ADHTGHEQQRAARLALSTAHWFPEEVDYVNACGLGTMELDALETNTVKQVLGDHAYRV
                     PVSSFKAALGHAFAASGAFQVIGTAKAMEHQFIPPTLNLTTPDPTCDLDYVSGQGRAA
                     HLDRALINSFGFGGKNIVLALSRVNAGIATTNPMAASPGYSMTHLVGVS"
     gene            complement(4514..5785)
                     /locus_tag="PK224_14580"
     CDS             complement(4514..5785)
                     /locus_tag="PK224_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17018.1"
                     /translation="MKQLAAQPAAMDATCTLLDLLQQRKTRRFGRGMTLPGGPLQYTS
                     QHDPLPLSREEERYLIYAAIGRSGRNLGDMQFVGRPGASAGQGHALMNFNSRTVPSPC
                     SAQSTQLFYTNDDGVFFVADAAGPDHPWDLEVVQLQSSRLEIPREAPFMLPFNQWYTN
                     RPGTTLFMPVTNIALLYLNLLLTMFSEDTGYFIVDTDNGNAACGLDAFRKSAGGHLHD
                     NLASRKMFSLRELDAGICETAVQEQGIICEHLSLMQQALGLGGGIQSVGSGRHLLGME
                     PHIYPGLGFHFVVPPGKPLRANPVGIPQVWEGPTPPFVPSMKDAVTSLVASKFGPNGT
                     YGKPSEQPWTNPKAAQQVPQHSERAIDATIAFADYVLGTYGRFPAHADACKSIVACQT
                     HHLDEEFYATFYPDSTLPDAHREHMHVWHSH"
     gene            complement(6007..6804)
                     /locus_tag="PK224_14585"
     CDS             complement(6007..6804)
                     /locus_tag="PK224_14585"
                     /inference="COORDINATES: protein motif:HMM:NF015437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRB17019.1"
                     /translation="MSQPAFSAKEFQRLGEIMHDARSVDRSDRVWQLVSAVQKVIPYE
                     FSGCGAVDLLRGIDPSLGHSTYPREFCHLYMGQGLAVDPAVNRLITSGQTVTSSADEP
                     TANEPKEIISLKLDFGIKTCLSAGVRGANGSCSYFAFSNFDAKQADKLRLLLDILTPH
                     FHLSYMRCHSAWNPERSAPPPTLLSKREEEILRWVAAGKTNWEISVILKVSLNTVKFH
                     LKNVFQKIGVENRWSAIAYWQTGEQHRIVPSAPPSDDRPPAGASTPD"
     gene            complement(7058..8038)
                     /locus_tag="PK224_14590"
     CDS             complement(7058..8038)
                     /locus_tag="PK224_14590"
                     /inference="COORDINATES: protein motif:HMM:NF013354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17020.1"
                     /translation="MRRLAVSALLLLCTLALPLPGSADTASEHVEWGAWSFDYEVRDN
                     SGLALRNVTYGGEQVLAKASMPVLRVKYVKERIWWNPFTWFGSRADSGRCGPFQDRLR
                     WQDLVPIVNCGDQKVCVESSTVKGMKWLELGIYALIGEYHIYQSWHLSEDGELRPVVQ
                     SRGLSCNTDHVHHPYWRFDFDINGNGMDQVFVRDDGGPDSGWGPGWKKYTNERNDVKH
                     TATHRAWFIRDQLTGHGVWVLPGDGYAPLKDDGERDSFADLDVAIRRANADEDLSWVF
                     GARGQLGYDEDNQGVQEQDVVFWYVAHLPHMAALGPTKWLTLGPTLKIHR"
     gene            complement(8057..8842)
                     /locus_tag="PK224_14595"
     CDS             complement(8057..8842)
                     /locus_tag="PK224_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17021.1"
                     /translation="MGTRPVMRTSAALMLLLLATGCSLDAASRGPFGPDDPETAVPKK
                     PLPAQLPTRHVLEFSSDESPEHIKPKMAGSALDAGVSRSPNREAVLSAADIAGLRKVA
                     ETDPRVAALLGERWGFIDADRVPPRGKVSFGCCRTSARFTRLTYFSYSHNVAVHVHMK
                     DSTILEASRAEGYLPPEGPRDIQRGIELARADRRLADKVQGMQGHGLLMQPDRGFFRN
                     DPGYGHRVIWITFSPGLGGDPKYWAQVDLTEDRVLDAGNEPPR"
     gene            complement(8848..9369)
                     /locus_tag="PK224_14600"
     CDS             complement(8848..9369)
                     /locus_tag="PK224_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17022.1"
                     /translation="MNRSIEGVVTFLQDNDSLIKQLMETAESATNEIEQLADRVGNAR
                     EEAQVLVDQLGAVEFSVGRQDDTKVRQAALLEAVAALRKKFETYKNDPLRRGIYAAEI
                     SNLYLQLANTFNNETVAQIVPFGADEISGYKDLMKKAVLDAASRKKRAAVIKAGVQIS
                     RLALGVAGKLALV"
     gene            complement(9380..10090)
                     /locus_tag="PK224_14605"
     CDS             complement(9380..10090)
                     /locus_tag="PK224_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17023.1"
                     /translation="MVTRGVRLLSVVILFLMATGCHSIHSRSVRDLIQLEGTKIDAAQ
                     TNIDLFQKETEARIKFLEQARSSLHESFKSLQIQETKHRFALASFRNLTTKKGDSAYA
                     AAYLTSLIYMADAQGLEKAVWNQFEEDFCGLRDTANALNDSWKRTATLHAQVHRFAKQ
                     TAFASVDPEFVTAVLDQAPNRSGQIMEILDHSRTVNDALEEVVGARILRIGGLDRAQL
                     FTADLVELLDRLKKDDGQ"
     gene            complement(10126..10758)
                     /locus_tag="PK224_14610"
     CDS             complement(10126..10758)
                     /locus_tag="PK224_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17024.1"
                     /translation="MRMLRIVHILIFASLAACTTPPEVKQALTAKDQGYTENEQLMQQ
                     YRELVGNINSRHVQWFRYVQTRLKLDLALQWATTNPRLTDIPDATLADDDAEVLGPDV
                     LAVINAMRLKSLPERKGANGQPVFLAGSGDMSHLIQQIPELISRIEQRVASDSKAPQA
                     LDLTAFDQYRNNVEALRRINGIIKQYLDIDVTLSRNDTQSLADALRTVRR"
     gene            complement(11072..12880)
                     /gene="uvrC"
                     /locus_tag="PK224_14615"
     CDS             complement(11072..12880)
                     /gene="uvrC"
                     /locus_tag="PK224_14615"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRB17025.1"
                     /translation="MTTDVLQSKLDHLPGQPGCYLFRNAKREILYVGKAAVLADRVRS
                     YFQRGSDQTPKTALLVNEIADLETIVTRSELEALILESNLIKRHRPRFNIVLRDDKQY
                     PYLRLPIKEHFPRLSIVRRVQKDGALYYGPYTPAGALRETLKVIRKAFPLATCEIEID
                     GRADRACIEFEIKRCMAPCTGNQSRDEYHQIVKQVRQFLEGRDRELLDSLREDMERAA
                     EREAFEEAARLRDRLFNVERTLEKQRITQVSTTDQDVIGLARQGTAADLQLLFVRGGL
                     LIGRKDFFWPQSTDSSDEELVRSAIEQFYNKEGQPPKELLVPTTLDEAPLIEQWLSEK
                     RGDSVRILAPERGTKHQLVLLAEENAAAAIADHLRNEALDRQATAELKRLLRLDTVPR
                     RIEGFDISNILGNQSVASLVVWEDGQAKKSDYRKFRIQTVEGANDFASMQEAVMRRYG
                     ATEDLARPDLILIDGGLGQLSAALEGLKQVGQDQIPIMGLAKARGEKEERIFLPGRKN
                     PIVLRPTSPATHLVQRIRDEAHRFALTYHRNLRGKALLSSELDQIAGIGTIRRKRLLK
                     QFGSLQRVATATDDELRSAGLDVSTVAALRKALTPS"
     gene            complement(12960..13829)
                     /gene="dapF"
                     /locus_tag="PK224_14620"
     CDS             complement(12960..13829)
                     /gene="dapF"
                     /locus_tag="PK224_14620"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRB17026.1"
                     /translation="MKNGFFRGHGLGNDYVVMDPTELTFKLTPGKIRGICDRHWGLGS
                     DGILALVPSKKADFGLRIYNPDGSEAEKSGNGLRIFARYLHATGKTKKRAFTVETKGG
                     LVSIALHLDRHGDAAAATVEMGRATFQPSALPCALTIDELIQQPIEAAGRSLQFTGVS
                     VGNPHCVIFKQPGEAWTREDLLTLGPALENHSLFPKRTNVQLAVPTGPKDIFILIWER
                     GAGETQASGSSSCAAASAAVRLGLVKGPVTVKMPGGTLNIEVAQDFSLTMKGPVAEVA
                     RGALSPSFVRGLK"
     gene            complement(13990..14388)
                     /locus_tag="PK224_14625"
     CDS             complement(13990..14388)
                     /locus_tag="PK224_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB17027.1"
                     /translation="MKVTKLLHTRMRVSDMDQTIRFYQNVLGLEVLERKVSPRGSHLA
                     FLKVPNSEELIELCSFPASGPVTVQEDLVHLAFQVENLDDTIRALESKQIRITDGPTK
                     TSSGSRFIFIDAPDGYEVELIERPPGTALV"
     gene            complement(14478..14969)
                     /locus_tag="PK224_14630"
     CDS             complement(14478..14969)
                     /locus_tag="PK224_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB17028.1"
                     /translation="MQPIDSLTRTIIRDMRAEDRDPVIEILTGSDPWKRLGFTAVDWN
                     RIFAPLPAGRDTFVLEIEGNVLGIAIVRRKFLFGDYLELLGISPSAVGQGLGSRLLTH
                     VESLTFARANNMFACVSDFNDGARAFYRKQGYKEIGPMPNFLIPGYAEILLRKTTGPA
                     RKS"
     gene            complement(14936..15658)
                     /locus_tag="PK224_14635"
     CDS             complement(14936..15658)
                     /locus_tag="PK224_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB17029.1"
                     /translation="MRGKQFWVRLSVIGGLLLILQAPSAEAQVIKAGPASCPAVALTY
                     DLCPVRTASGFDWELIDFLIEHKVPATFFMSGRWMAKHDAEVKKLLAVPFFEIGTHGE
                     VHAHLPMHEVEEQQQEIMGPVRLLKTKYGRPAALFRPPYGEYNDHTVEAVKALGLKFI
                     LWNIESGDPDPTLTAEAIVARVQKRMKPGSVIVMHANGKGKHTREVTETLAATLLPAK
                     GLTPMTVSDLLRCNQSTVLPAP"
     gene            15731..16903
                     /locus_tag="PK224_14640"
     CDS             15731..16903
                     /locus_tag="PK224_14640"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRB17030.1"
                     /translation="MQGPDWKIGRMFGIPIHVHASWLFVFFFVTWSLATGYLPDVLPG
                     LTEPRYWAMGGVAAILLFASVLLHELGHSLVALRYRIPISQITLFIFGGVAQMRKEPP
                     HPRAEFLIAIAGPIVSFLLAGLCIGLVALLEFLPTGSSVHGLVALGTLLGMVNTQLGL
                     FNLLPGFPLDGGRALRAGLWAWSKDYYRATSQAALVGLLFGVSFGLFGAFLLVGALSG
                     TVSGALASSGGWIVLLGAFLFAAARGSRKQAAIRGSLASVPVRELMVSNVVALSPDTT
                     LEEAVNQYFLPYGYGGFPVVQDGRLLGVVGVRDVQTVNNSLWAFRRVADIMQTSSGDM
                     VVSPDLSAMQALEQMLALGAERLVVVQDGQLLGLVTRASIGHFIEQRHPSGMGSSS"
     gene            complement(16959..17819)
                     /locus_tag="PK224_14645"
     CDS             complement(16959..17819)
                     /locus_tag="PK224_14645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRB17031.1"
                     /translation="MTELSFLSNVFDTFRRRGCLSLAASLAFFSLLSLFPMVFLLLYG
                     ISFIVSQDVIGYQFLLGFLKGFLPSMGERLARDIRRVAEQEEVRWAVFLAFGWFSALV
                     FYELDYAMNTVFGTAAKRHPLISTLVAVALIWMLGFLTLISFVATQAIELLTAYAPRF
                     LGMNLVAIAAHDFLIAYSLPFLLAFASVTCLYRFLPHQRPSWREATIGGAVFSLLWVS
                     AKALFVTYLEDAAVYTQLYGSLLEVVLLMLWVYYSSALVLLGAVVTHECQCRRPSYSK
                     EASPDRAEVV"
     gene            complement(17949..19424)
                     /locus_tag="PK224_14650"
     CDS             complement(17949..19424)
                     /locus_tag="PK224_14650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRB17032.1"
                     /translation="MTFPLQDLLTILPELIVIGTACLILALDPILEASKKDVLAWLTL
                     GALAMCIGLTSSQMTGRVSAFSGMVVIDAYAAFWKLLLYIVTGLTVLLSLAYLKAERL
                     SIGEYYGFILLALAGMMVMVSGADLLTIYLGTELMSLSLYVMAGLKRTEARSLEASAK
                     YFVLGAFSSGILLYGISLLFGLAGSTGLSAIAAAITTHGTGDPLLSMALVLLAVGFGF
                     KMAVVPFHMWTPDVYQGAPTSVTAFMAVASKAASFGAFLRVFVEGLGAASSDWSVLFT
                     VICLATLALGNLVAIVQTNIKRMLAYSSIAHAGYALIGVVVAGGHSGEGASTSFASVL
                     LYIAIYSFMTLGAFALIGMLRKEGQESEQIEDYAGLAKREPLAAFFMLVFMVSLAGIP
                     PTAGFIGKFYIFMAAVNGGMTWLAIVAVIFAAISAFYYLRVVMVMYMREAEGSVATHS
                     RLETSPALSLVLACALAGVVLLGLFPNGLWSLASQAAPLLK"
     gene            complement(19421..20989)
                     /locus_tag="PK224_14655"
     CDS             complement(19421..20989)
                     /locus_tag="PK224_14655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRB17033.1"
                     /translation="MTSFPWLSLIVFLPLAGALLCLLVKAESARWLALGVTVADFALS
                     LPLWWLFDSSTAGMQFVERASWISSPPVQYSLGLDGISFPLVLMTTFLMPFCVTVSWT
                     AIEKRVQLFMSMLLVMETAMLGVFVALDFVLFYVFWEAMLIPMYLLIGVWGGPNRLYA
                     AIKFFLYTLAGSVLLLVAILALYFQGGHTFDILALSRGTYGASLQMWLFLAFFAAFAV
                     KVPMFPFHTWLPDAHVEAPTAGSVILASVLLKMGTYGFLRFTLPMLPDATVSFTKPMI
                     ALSIIAIIYGAYMALAQTDIKKLIAYSSVSHMGFVTLGIFVLNIQGIEGAVMQMVNHG
                     ITTGGLFLCVGIIYERTHSRQIQDNTGLAWPMPRYAIFLMIFALSSLGLPGTNSFVGE
                     FLVLAGTFVWSQFATALAALGVILAAAYILWLMQRVVFGTPSAAHRAHLLDLNARETA
                     TLVPLIVLVFALGIFPNPLLSRMHASVTQLLGGPKNPTAAVEQQQAPVTAGKPTAAIA
                     AILSPSSSEQAAAR"
     gene            complement(21017..22915)
                     /gene="nuoL"
                     /locus_tag="PK224_14660"
     CDS             complement(21017..22915)
                     /gene="nuoL"
                     /locus_tag="PK224_14660"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRB17034.1"
                     /translation="MLYALIPFLPLFAFLIVGIGEQWIKDRAHLVAVPAMVGSFLLSL
                     LALHDVATGQAINVPLYTWLTSGNLDIHIGISIDRLTAVMLILVTTVSTLVHIYTIGY
                     MHGEPGYARFFAYIALFTFSMLMLVMADNLLQLFVFWEAVGLCSYLLIGHWYERASAC
                     AAATKAFLVNRVGDFGFILGLFLVWYSFGSLDYATVFAHAQDLASKTTNLLGPFGGTW
                     DVSVMTMICLLLFTGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVAR
                     LSPLYNLSPTAMTVVALVGGLTMMLGATIALTQTDIKRVVAYSTMSQLGYMVMACGLG
                     AYSAGMYHLLTHGAFKALLFLGCGSVIIALHHEQDMRHMGGLKDKLPVTYWTFLVGSL
                     ALAGFPLTAGFFSKDDLLVSSWSSGPLGQVLTICGLLTAGLTAFYSFRLVFVTFWGKS
                     RVDPHHAGHVHEPSTTMTAPLLVLAVLSIVAGYLGIPAFLEPVFHGDGAAAHHEGSAA
                     YGIMAVATLMGLTGIGAAYYLYVLNPGLPDRLAQQWRAAYELSLHKWYIDEAYDRSLV
                     RPTLSAAQGLWKHVDVAIIDGAVNGVARAIAWGGWLIRLTQSGQTQHYALGMTLGAVV
                     ILTVYLLF"
     gene            complement(22932..23237)
                     /gene="nuoK"
                     /locus_tag="PK224_14665"
     CDS             complement(22932..23237)
                     /gene="nuoK"
                     /locus_tag="PK224_14665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRB17035.1"
                     /translation="MAVPLSYYLILSGFVFLTGVVGVLIRRNIIVILLSVELMLNATN
                     INFVAFSEYFHNVAGQVFVFFALTVAAAEVAVGLAIIIALHRSNSSIYVDDLNLLKR"
     gene            complement(23252..23764)
                     /locus_tag="PK224_14670"
     CDS             complement(23252..23764)
                     /locus_tag="PK224_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRB17036.1"
                     /translation="MDHIFFFYFAAVIAATSLLVVALRNPVYSALSLLIMFFHVAGLY
                     ITLHAEFLAAVQIVVYAGAILVLYLFVVMLLSIKSEERYHNQLPVAGLLGVVLCTEVI
                     LLFIKSQTRGMAPAAAADPVAGPGNTEMIGEALYSTYLFPFEVASLILLVAMIGAIIL
                     AKKDINEPVQ"
     gene            complement(23895..24470)
                     /gene="nuoI"
                     /locus_tag="PK224_14675"
     CDS             complement(23895..24470)
                     /gene="nuoI"
                     /locus_tag="PK224_14675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRB17037.1"
                     /translation="MNVAVTTQPKRKPSFSDWLKTLTFYELLVGMKATLTHLLNYKPI
                     TLQYPHEKRLLPDNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSGEVPGEPT
                     KRYSKEYYMDMTRCLFCGLCVDACPVDALGMTREFEWAVYDKRQLHLNKQQLLAIGDR
                     SFPVREKRLELQHPNVAFFNVTFTHLPQKEN"
     gene            complement(24480..25547)
                     /gene="nuoH"
                     /locus_tag="PK224_14680"
     CDS             complement(24480..25547)
                     /gene="nuoH"
                     /locus_tag="PK224_14680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRB17038.1"
                     /translation="MEIGLRLAVSLAQIAAVMGVVMLTVMVLTLAERKVLGWMQDRMG
                     PMEVGPYGVLQPIADGLKLFFKEDIIPAGANKFLFTLAPILALVPAMIGFAVIPFGPS
                     LTIELFGMQIKPFVISDINIGILYILAFASIGAYGIILGGWSSNSKYSLLGGLRSAAQ
                     VISYELCVGLAIVGVILLSGSLSLVKITEAQAGGFWNWFVIAMPFPQIFAFVVYVISA
                     VAETNRVPFDLPEAESELVAGFFTEYSGMRFAFFFIAEYANMILVSCVAAALFLGGWN
                     APYPGTILGYIGLDSLAWIENVVWFAAKVYFFLFLFFWLRATLPRLRYDQLMRFGWKV
                     MLPIALGNIVLTAIAAYFFPR"
     gene            complement(25572..28241)
                     /gene="nuoG"
                     /locus_tag="PK224_14685"
     CDS             complement(25572..28241)
                     /gene="nuoG"
                     /locus_tag="PK224_14685"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRB17039.1"
                     /translation="MPDPKPETVRLTIDGTTVAVPKGTLVIEAARRVGVMIPHFCYHP
                     KLKPDANCRMCLVEVEKMPKLQTACSTPVAEGMAVRTATTTVDDAHKSVLEFILANHP
                     LDCPVCDQGGKCDLQDFSHQYTPTTSRFTETKRIFQKEYFSPLIETQMNRCVQCLRCV
                     RYCDEIMDVKALAPVGRGTMTEIKHFGPHELDCEFCGGCIQICPVGAITSRLSMYEYR
                     PWMLKRADTICTFCGDGCRITVQTKDNELIEVNSAHGAGRNNGDLCARGFFGFHASSH
                     ADRLTHPLIRREGKLVETTWEDALEYVAEQALRLKLAQGADAFGGLISSRCTNEDLYV
                     FQKFMRQVIGTNRIDSSARYGHLNGVQALRRVQGTHRWTIAFEDIVAANALLLVGTNI
                     TETSPITGLKVKEAVKKRQASLVTVETLQPAIDTLSNITNLALQHFPTHPAQFGNTVL
                     GVIKAVIEGNLVDAAVAQQAPAFVQRLTSALQGTSWEALEAATGQNRAQWAEAATVLA
                     KANRLVILVGNGVLRHPGGDATVTNLLDLLILLGKLDKAGCGLGPLAEENNDQGAVEM
                     GAVAEFFPGPAPLDDQPVRDRIAAMWREDLPRTPGASLTEMLAAANKGTLKAMFVVGE
                     NPVGTLPAAAQAKEALGKLDLLVCQELFLTETAAIAHVVLPACSYMEKDGTFTNSEGH
                     VQGVRQAINPVGDSRPDWEMLSALSVLMGSPLEYGDAREILKEIRTVIPGYGLLGPTP
                     TPPKVDPMVLNRYLTEGFAVDAATRYAVSQPRQTNDGPFTLMFVQTLFHSGKLSTRAK
                     GLLQLQQEGFLSINPADAARLGLADGGQVKVSNSRGAITTTVKIRERVPAGLLWFPEH
                     FDGEAKQLAEWTIDPRTQIPYFKLAHVSLTKVS"
     gene            complement(28353..29660)
                     /gene="nuoF"
                     /locus_tag="PK224_14690"
     CDS             complement(28353..29660)
                     /gene="nuoF"
                     /locus_tag="PK224_14690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRB17040.1"
                     /translation="MPKYEPILLKNMLQPGYTGSLTEYERVGGYQAIRKVLGKVSPAD
                     VTAVVMKSGLRGRGGAGFPTGVKWGFLPKDYQGPRYLCCNADESEPGTFKDRQLIERD
                     PHQLLEGMLLACYAIGAASSYIYIRGEFVLGAKILEQAINDARAAGYVGKNIFGSNTT
                     IDIWVHRGAGAYICGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLAN
                     LPHILNRGAEWFASIGSPPKSTGTRVFCVSGHVARPGNYEVPMGITFRELIYEQAGGM
                     RSDKKLKAFIPGGASAPFLTPDHLDVKLDFESVALAGSMLGSGGVTVMEEGTDMVWAA
                     LRLMEFFYHESCGKCSPCREGSSWLVQIMRRIVNKRGQMADLETLTDLCKNIAGRTVC
                     AFGDAEVSPILSTLKHWRHEYVAMIHAAEAANLIRPEPAGAKH"
     gene            complement(29734..30273)
                     /gene="nuoE"
                     /locus_tag="PK224_14695"
     CDS             complement(29734..30273)
                     /gene="nuoE"
                     /locus_tag="PK224_14695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRB17041.1"
                     /translation="MLKETHKAEIEDILSRYPVKRSALLPLLYMAQREQGYVTEAAMQ
                     EIAGILRLTPPQVYETATFYTMLNLKPVGTFHLQVCKSLMCALVGSDTIIGWISAKLG
                     IKPGETTPDKLFTLSIVECLAACGTGPMMQVNDDYYERLTEEKLDRILADLRQTGTCS
                     LKTGPFMWPEPANAVKREA"
     gene            complement(30338..>32093)
                     /gene="nuoD"
                     /locus_tag="PK224_14700"
     CDS             complement(30338..>32093)
                     /gene="nuoD"
                     /locus_tag="PK224_14700"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRB17042.1"
                     /translation="KMQPLLERLMTTFPDAVRGVEVDTARNEITARVAAARIVDVARW
                     LHDTPEASFDHITDICSVDYPNDPERFEVVYQLLSLAHRRRIRLKARVTEDAPQIASV
                     TGIWKGAEFMEREVYDLMGITFVGHPDLRRILLPEDYEEGHPLRKDFPTEGRGWRSSF
                     PFIPRLDEAPEEQTEGEVPEQEKQAYLVAEHQGGTRRREELLLNMGPQHPSTHGVLRV
                     VLELDGERIVKATPDLGYLHRGVEKLAEGLHYMQVIPHTDRLDYVCAMTNNYAYVRAV
                     EKLLDITVPERAEYVRTIVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFREREVLLDM
                     FEKLCGARLTLNYYRIGGVDSDFTPDLVVRLKAFLQTFPEKVNEYNQLLMSNRIWVGR
                     TKDVAVISAEDAINFGLTGPSLRGSGVDYDVRKYEPYGVYDKVEWEVPVGKRGDTYDR
                     YWVRMEEMRQSARIIAQCLDQMPEGPIMADVPHVIPPPKAKVMRDMESLIHHFIIFTQ
                     GFKPPKGETYCGTEAPKGELGFFIVSDGSPRPYRLKIRAPSFIHMGAFDHMARGYLIS
                     DIITIFGTYDIVMGECDR"
BASE COUNT         6224 a         9286 c        10196 g         6387 t
ORIGIN      
        1 agatggtttc aaaggcgggg ctgacataga ggacctcgct cttatccggc gaggtgagcc
       61 agaagacggc atcgatatgc tcagctaatt gccggaatcg ctcttcactc tcccgaaggg
      121 tcgcttccat gcgcttccgc tcggaaatgt cgcgaagcat gcccgtatac tttttctccg
      181 ttccgatctg catctcgctg accgacacat gcacgtcgat ccgagtcccg tctttgcgca
      241 cagccggaag gtcgcggccg ctgccgatga tggtgcgttt cccggtgacc aggtagttcg
      301 ccaggtactc atcgtggtgt tcacgatagg gagagggcat gagtagggaa atgttctgcc
      361 cgaccagttc gtgcgcctgg taccccagca aattcagcag ggcggtgttg gcgctttcga
      421 tccggccgcg ttcgtcgatg acgacaatgc cttcgatcgc gctctcgaag atcgaacgca
      481 ttctggtttc gctgtcccgc caggcaaatt cggcgcgttt gtagtcggtg atgtccagca
      541 cggttccggc catactcacc ggtcgtccgt cggggtcgcg ctggacgact ccgcggcaat
      601 tgacgtgccg gacttcgccg tcggggcggc gaatccggca atcgacgttg tagggcgagt
      661 cgtatttcag cgccgcatcg accgcttgct gtacgcgctc gacgtcgtcc ggatgcagcg
      721 cggagaggaa ggtcgcatag gtcgggaggc tctggtctgg agtatggccg aagatccggc
      781 actgctcttc ggaccatgct tcctgaccgg tcgcaatatt ccagtgccac gaaccgagat
      841 gggccagcgt ctgggcctca tccagcgcct gttgtttggc ctgcaagtcg cattgcgcgc
      901 gtgcctgcgc cgcttcgagt gccttacggt cggaaatgtc gagatggatg ccggcggccc
      961 gcaagggggc acctttagcg tcccgcttga ccacgcgtcc gctgtcgaag acccagcacc
     1021 aggcgccgga cttggttcgc aggcgatgct cgctggaata ggtgggcgtt tccccacgaa
     1081 gatgcgaaga aaccgtcgcc atcacctggt gaaggtcatc cgggtgcacc aattgttccc
     1141 aggtccggac atggggttcg agctcgtcga gacggtagcc gagcatactc gcccatcgtt
     1201 cgttaaaggt cactcgcccc gtggggattt cccagtccca ggtgcccatt tgggaacctt
     1261 gcacgatcag ctccatccgg tcggcctgct cgtccacagt ctgttcatgc acagcgagaa
     1321 ggcgttcgag gtcggcgatc ctggcctgca gatgcgcgtc gtggacgggc gttgggtcgc
     1381 cgccggcctg ctccggcttg gaagtgtggt gcgggtgaaa ggccgccgtc gtagtcgcat
     1441 agggcgccag tgcaatctgc gcgcacagcg cgaggggttt gaagagggtt gccgtgcttt
     1501 cagggataaa gtccttgctg aagagaatga tggaaaacag ttcaccgtcc ggcagcggcg
     1561 cgccgaatcc caggaccgac cggatcccgt acttgagcac gaactcttcc tgtcccggga
     1621 cgtaggggct tccttcggct tcgggaatgt gaaagacgtt gaaggattgt tcgtgctggt
     1681 cgagcaacac gcttggaccc ggctgtgtga gggaggggag cgagaccccc aattgtcgga
     1741 agagctggct gaacatgggc aaccgttcga cggcctccag ggtgtcgagc gggatcacgc
     1801 ggaagcggct cgaacgcgcc gggtcattcc acccggggac cgctccagcg ctggccagca
     1861 gggtgaggca ggtcagagag gggggcgggg gcgtgtctcc gagccgggcg tcagccagtg
     1921 cacgaaggtc cggtgtcagc cggctatagg gtgtcgtctt gaagaggcgg accaagctgc
     1981 aggccggctc ccggagatgg cccatggtca aggatccgta gaggtgacga acgaggcggt
     2041 cggcgacctg ctcaaggctg gtggcatctg tgccgagccg gcgcaacgcc gcgccgcagg
     2101 cggtcatgtc tcggagttgg aacgatcgca gatggtgcat gctacaggcg attccgcaaa
     2161 ggcagagcgt aggcgtcgat actctgtcgg tcggcgggag gaggaacttg agcaccgcac
     2221 catcgtgcgt caggcgatcc ggcgtcaggg aatggttttt aagtaaaagg cggcggcttg
     2281 agacctcctg ctcaggtgaa gtttcgaata cacgttggcc aggtagtttt tgactgtttt
     2341 atcgctcagc ccgagctcgg cggcgatttc cttgttcgtt tttccttgtg caatgagggg
     2401 gaggaggcgt cgctcctgcg gcgacagcag atgccggccc cgttcgggtt cacgctcggc
     2461 aactttgcga agataggtaa aggtatgacg ggtgactccc gggtccaggt aggattgacc
     2521 cttggtcagg ctgcgcatgg cgtgcagcag ggtatccacc ccggcgtcct ggaggacata
     2581 cccgtgggcg cctgtcgcga cggcggagag cagggtggaa tcagtgagtg catcggccag
     2641 aaagagcacg cgcgtctccg gaagatgcgc gaggatgtcc ttggccgttt cgatacccgt
     2701 gccgtcctgc acgcgcagat cgagcacgac gatgtcgggt ttgtggtgga gcaccagggg
     2761 gagcgcgtcc tctttcgacg aggcctctcc gaccaccata aagtccggta cggagttcag
     2821 cagcgttcgc atgccgactc tgatcaattc ttgaggatta atgatgaaca cgcgcgctgg
     2881 agtgttctgt atttcgttca tattcgactc tcaactaaat gtggatggat gactggtgaa
     2941 ccgtttctct gtctgtgccg aggcggcggg tgcccttcga taaggggggc ccgtcggatg
     3001 atgatcggcg tccatcctgc tctaaccgga gagaagccgc gatcgaacgg ccctgcggcg
     3061 ggccggcctg tcagaggccg acccgcgcac acggccgcgc attcgatgcg cgatgtcttc
     3121 tggctcaggt tgctcatggt gcgggcctac gagactccca ccagatgggt catgctgtag
     3181 cccggcgacg cagccatcgg gttggtggtg gcgatgccgg cattcacgcg cgacagcgcg
     3241 agcacgatat tcttgccgcc gaatccaaag ctgttgatca aggcacgatc gagatgcgcc
     3301 gcccgcccct gtcccgagac atagtccaga tcgcatgtcg gatccggcgt cgtcaggttc
     3361 agcgtgggtg ggatgaactg gtgctccatg gccttcgcgg tgccgataac ttggaaggcg
     3421 ccgctggcgg cgaaggcgtg gccgagtgcc gctttgaacg aactgacggg aacccgatac
     3481 gcgtggtccc ccaggacttg tttgaccgtg ttcgtttcga gcgcatctaa ctccatggtg
     3541 ccgagcccgc aggcgttcac gtagtccact tcctccggga accaatgggc cgtcgagagg
     3601 gccagcctgg ccgcccgttg ctgctcgtgt cccgtgtggt ccgccttcac catggaatgg
     3661 gcatcacagg taaagcccca tccttccagc tcggcataca tcttggcgcc gcgggcgcgc
     3721 gcgtgctcgt agtcttccaa gatgaggcag ccggcgcctt cgcccaacac catgccgcat
     3781 cgggttttgt cgaaaggacg gggaaggttg cgaatggccc cgcgttcgtc cggcgccagc
     3841 gcgcgcccgg cgttcatggc ggcgtagacg ctcgggtgga gcggcgcttc ggtcccgccg
     3901 gccacgacga gcgacgattg gcccgacttg atccattcgt aggcgcggcc gatggcattg
     3961 gccgtcgagg agcagccgat cgagtacgtc tcgcaggggc cgtgcagccc gagcacgatg
     4021 gccacttccg acgaaatcgc attcgggaag gtcatcccca tggtgaatgg attgacgcgc
     4081 tcataggcgt tgagtttggc cgcatcgtgg aactcgaacg cttccttcat gccgccgatg
     4141 gaggtgccga tgctgatgcc gatggacccg cgatcttcct gttccagctc gacgccggaa
     4201 tcgcgggctg ccatcatggc ggaggcgacg gcgaactggg tggcgcgccc catacgacgc
     4261 gcctgcttgc gatgcaggaa atcttcgggg tcgaagtccc gcacctccgc cgcgaggcag
     4321 gcttggaacg gtgaggtatc gaatgagctg accggtttga cggcgctctc acccttgctc
     4381 aatagatgcc agaacagatc gacggtacaa cccaacgggg aaatcactcc cattccggta
     4441 atgacgactc gacgagaggt ctgtggtgtc atggtgccct cctgtgctcc ggggagcgtg
     4501 aaaatgtgtg tcgtcagtgg ctgtgccaca catgcatgtg ctcgcgatgc gcgtccggta
     4561 acgtggaatc gggataaaac gtcgcataga actcttcgtc caggtgatgg gtttgacagg
     4621 ccacgatgga cttgcaggca tcggcgtgcg ccgggaaccg tccgtaggtg ccgagcacgt
     4681 agtcggcaaa cgcgatggtc gcgtcgatgg cgcgctcgga atgctgcggc acctgctgcg
     4741 cggctttggg attggtccaa ggctgctcgc tgggctttcc gtaggtgccg ttcggcccga
     4801 acttgctggc gaccaggctg gtcacggcat ccttcatcga cggcacgaac ggcggggtgg
     4861 ggccttccca cacctgcgga atgccgaccg gattggcgcg caggggcttg cccggcggga
     4921 cgacgaagtg gaacccgagg ccggggtaga tgtgcggttc cataccgagg aggtggcggc
     4981 cgctccccac gctctggatg ccgccgccca gtcccagggc ctgctgcatc agcgagagat
     5041 gttcgcagat gatcccttgc tcttgaacgg cggtttcgca aatgccggcg tcgagctcgc
     5101 gtaggctgaa cattttgcgc gaggcgagat tgtcgtgcag atgtccaccc gcgctcttgc
     5161 ggaaggcatc gaggccgcag gcggcgttgc cgttgtccgt atcgacgatg aagtatcccg
     5221 tgtcttcgct gaacatcgtc agcaagagat tcaggtacag cagcgcgatg ttggtgacag
     5281 gcatgaacaa ggtggtgccg ggccgatttg tataccactg attgaacggc agcatgaacg
     5341 gggcttcgcg cgggatctcc agccgggacg attggagttg caccacctcc agatcccagg
     5401 gatggtcggg accggccgcg tcggcgacaa aaaacacacc gtcatcgttg gtgtaaaaga
     5461 gttgcgtgct ctgcgcggag caggggctgg gaaccgtgcg gctgttgaag ttcatcagcg
     5521 cgtgaccctg gccggcactc gcgcccgggc ggccgacaaa ctgcatgtcg cccaggttgc
     5581 gcccggatcg gccgatggcc gcatagatca gatatcgttc ttcttcccgg ctcagcggga
     5641 ggggatcatg ttgactcgtg tattgaagcg ggccgccggg cagggtcatg ccgcggccga
     5701 aacggcgcgt cttgcgttgt tgcaggaggt cgagcagagt acatgtggcg tccattgctg
     5761 caggttgtgc ggcaagctgc ttcatagggc ctctccttcc cggcatccta gggcaggatg
     5821 gccccggccg ggtgtgtgaa tgagggtggt gctggtacgt cacgcgcggc atgacgttgt
     5881 gtgggacgac tgtacgagag gcgtgatctg gcgtcaccta cccatcgggg taggcgatcg
     5941 ccctacccag cgtcggtcaa ccggtgacgg acggggcgga gcgcgagtcg gcatgcatcg
     6001 agcgatttag tcgggtgtgc tggcgccggc gggcggacgg tcgtcactgg gcggtgcgga
     6061 tggaacgatg cgatgttgtt cgccggtttg ccaataggca atggcgctcc atcgattttc
     6121 gaccccgatt ttctgaaaga cgttcttgag atgaaatttg acggtgttca aactgacttt
     6181 cagaatgacg gagatttccc aattggtctt gcctgccgcc acccatcgga gaatttcttc
     6241 ttcccgtttg ctcagtaggg tcggcggcgg ggcagatcgc tccggattcc acgcggagtg
     6301 gcatcgcata taactcaggt gaaaatgcgg ggtcagaatg tcgagcagga gccggagttt
     6361 atcggcttgt ttcgcatcaa aattactgaa ggcgaagtag gaacaggatc cattcgctcc
     6421 acgcactccg gccgacaggc aggtcttgat gccgaagtcg agtttcagcg agataatctc
     6481 cttgggctcg ttggcggtcg gttcatcggc gctggaggtc acggtctgac ccgaggtgat
     6541 gaggcggttg acggctggat cgaccgctaa tccttggccc atatacagat gacagaattc
     6601 ccgcgggtag gtggaatgcc ccagtgaagg atcgatccca cgcaggagat cgacggcgcc
     6661 gcatccggag aattcgtagg gaatcacttt ctgcacggcc gagacgagtt gccacaccct
     6721 gtcgctgcga tcgacggagc gtgcatcatg catgatttcg ccgagccgtt gaaattcctt
     6781 ggcggaaaat gctggctgac tcatgccgat tcttcccgat cagcggggcc gggtgcgagt
     6841 gggagcaccc gaggccatga agattcagaa gcgcggacgt ggttcatagt gagacagctg
     6901 tgccctgatg aaacaggctg attgtactgt ccgcttgagc ccggtatcta gggcaacctg
     6961 cacctagtat agagtgctta aggtttgaac cgcaacgggg aagaggaggg tcttgaaaat
     7021 tgacatggcg ccggcgggag gcccgcaggt cgtcggacta gcggtgaatc ttcagggtcg
     7081 gcccgagcgt cagccacttc gtggggccga gggcggccat gtgcggcaga tgggctacgt
     7141 accagaacac cacatcctgt tcctggacgc cctggttgtc ctcgtcataa ccgagctggc
     7201 cccgggctcc gaagacccat gagagatctt cgtccgcatt ggcccggcga atggccacgt
     7261 cgaggtcggc gaaggaatcg cgttctccgt cgtctttcag gggcgcgtag ccgtctccgg
     7321 gtaacaccca taccccatgt cctgtgagtt ggtcgcgaat aaaccaggcc cggtgagtgg
     7381 ccgtatgttt cacgtcattg cgttcattgg tatatttttt ccatcccggc ccccagccgc
     7441 tgtccgggcc cccatcgtcg cgcacaaata cctgatccat tccgttgccg ttgatgtcga
     7501 aatcgaagcg ccaataggga tggtggacgt gatcggtatt gcaggacaac ccccggctct
     7561 ggacgaccgg ccgcaattca ccgtcttcgg acagatgcca ggattggtaa atgtgatact
     7621 ccccgattag ggcatagatg cccagttcca gccacttcat gcctttcacc gtcgagcttt
     7681 cgacgcagac cttctggtcg ccgcagttca caatcgggac gagatcctgc cagcgcagtc
     7741 gatcttgaaa ggggccgcag cgtccgctgt cggcgcggga gccgaaccag gtaaaggggt
     7801 tccaccagat gcgttccttc acgtatttca cgcgcaggac cggcatgctg gctttggcca
     7861 gcacctgttc tccgccgtag gtgacgttgc gaagggccaa gccggaattg tcccggactt
     7921 cgtagtcgaa actccacgca ccccactcga catgctccga tgctgtgtcg gccgagccgg
     7981 gtagcggcag cgcgagggtg cagagcagta acaaggcact caccgcgagg cgtctcatcc
     8041 cgtatccttt caccgcctaa cggggcggtt catttcctgc atccagcaca cggtcttccg
     8101 tgagatcgac ttgtgcccag tatttggggt cgccgcccag accaggggag aaggtgatcc
     8161 aaatcactcg atgtccatac ccggggtcgt ttctgaagaa accgcgatcc ggctgcatca
     8221 acagcccatg tccttgcatc ccttgcactt tgtcggcgag acggcggtcg gcgcgagcca
     8281 gctcgatgcc ccgttggata tcccgggggc cttcgggggg tagataccct tccgctcgcg
     8341 aggcttccag aatggtcgaa tctttcatgt gcacgtggac ggcgacgtta tggctgtagc
     8401 tgaagtaggt gagtctggtg aatcgcgcgc ttgtccgaca gcagccgaac gacaccttcc
     8461 ccctcggtgg tactcgatcc gcgtcaatga atccccatcg ttcaccgagg agggccgcga
     8521 ctcgaggatc cgtttccgcg acttttcgca gtcccgcaat gtcggcagct gagagcaccg
     8581 cctcccgatt gggtgagcgg gagacaccgg catcgagtgc gcttccggcc atcttgggct
     8641 tgatatgctc gggggattca tcggatgaga attccaggac atgtctggtc gggagctgtg
     8701 cgggcagtgg cttctttggc acggcggttt ccggatcgtc cggaccgaag gggccgcgtg
     8761 aggcggcatc gagactgcag cctgtggcga gaaggagcag catcaacgcg gcggatgtcc
     8821 gcatgaccgg tcgagttccc atacctctca caccaatgcc agtttgccgg ccacgccaag
     8881 tgccaaacgc gaaatctgca cgcccgcttt aatgacggcc gcgcgttttt ttcgcgacgc
     8941 ggcatccagc acggccttct tcatcaagtc tttatagccg ctgatttcgt cggcgccgaa
     9001 ggggacgatt tgggccactg tctcgttatt gaaggtgttg gccagttgca ggtacagatt
     9061 ggagatctcc gcggcataga tgcctcgtcg cagcgggtcg ttcttgtagg tctcaaactt
     9121 cttccgcagg gcggcgacgg cctccagcag ggctgcttgc cggaccttcg tatcgtcctg
     9181 gcggccgaca ctgaactcca ccgcccccaa ctgatcgacc agcacttgcg cttcctcgcg
     9241 ggcgttgccc acgcgatcgg ccagttgttc gatttcattg gtggcgcttt cggcggtttc
     9301 catcagctgc ttgatcagac tgtcattgtc ttggaggaaa gtgaccaccc cctcgatact
     9361 gcggttcata gagcctccgt tactgtccgt cgtccttctt caatcgatcg aggagttcca
     9421 ccaggtcggc ggtgaacagc tgagcacgat ccagtccgcc gatgcgcaga attcgcgcgc
     9481 cgacgacttc ttccagggca tcgttcacgg ttcgcgagtg atccaggatc tccatgatct
     9541 ggccggaccg gttgggggcc tggtcaagga cggccgtgac gaactcagga tcgacggatg
     9601 cgaaggccgt ttgcttggca aatcgatgca cctgggcatg cagcgtcgcc gtccgtttcc
     9661 atgagtcgtt gagggcattg gccgtatcgc ggaggccgca aaagtcctcc tcaaattgat
     9721 tccagaccgc cttctcgagt ccctgggcat cggccatgta gatgaggctg gtgaggtagg
     9781 ctgccgcgta ggcggagtct cccttcttcg tggtcaggtt ccggaaggag gccagggcga
     9841 accggtgttt ggtttcctga atctggagtg atttgaagct ctcgtgcagc gaactgcgcg
     9901 cctgttcgag gaacttgatg cgggcctcgg tttccttctg gaagaggtcg atgttggtct
     9961 gcgcggcgtc gatcttggtg ccttccagtt gaatgaggtc gcgcaccgaa cggctgtgga
    10021 tcgagtggca accggtggcc ataaggaaca gtatgacgac ggacagcagc ctcacgcctc
    10081 gcgtgaccat ggagggctcc ttcttccggc cggctgtgcg gcggatcatc ggcgcaccgt
    10141 gcggagcgcg tcggcaagcg actgggtgtc gttccggctg agagtgacgt cgatgtccag
    10201 gtactgtttg atgatgccgt tgatccggcg tagcgcttcg acattattcc ggtactggtc
    10261 gaacgccgtg agatcgagag cctgcggggc cttggaatcg gaggccacgc gctgctcgat
    10321 ccgcgagatc agttcgggaa tttgctggat gagatgactc atgtccccgg agccggcgag
    10381 aaaaacaggc tgcccgttcg cgcccttgcg ttcggggaga gatttgaggc gcatggcatt
    10441 gatcacagcc aggacatcgg gacccagcac ctcggcatcg tcgtcggcga gggtggcgtc
    10501 cgggatgtcg gtcagccggg gattggtcgt ggcccattgc aaggccagat ccagcttgag
    10561 acgagtctgg acatagcgga accactgcac atggcggctg ttgatattgc cgaccagctc
    10621 ccgatactgc tgcatgagct gttcattttc ggtatagccc tgatccttgg ccgtcagcgc
    10681 ctgcttgacc tccggcgggg tggtgcaggc ggcgagcgag gcgaagatca gtatgtgaac
    10741 gatgcgcaac atcctcatcg ggatgcctcc ccattatcgt cttaagtcgg atgcccggag
    10801 agagacgcaa actacgtacc tggcaagcag caattgctcc gctgtcgaac gagtcgcaaa
    10861 aacccaatat tcgaagagaa agtgtgcctg ttccactgtg aggcatcgcc ggtgcggcgg
    10921 atcgagcgta cctattcaga tacgggtgta tcgaacccaa tacagtttat gcgaagtctc
    10981 tctcggtatt gcgttagctc gcattactca tgacggtgcg tcgccgcagc ggatcggaaa
    11041 ttgcagccga agcgctcatg aataatgtgg gctaggacgg ggtgagggct ttacgtaggg
    11101 cagcgacggt ggagacatcg aggccggcgg accggagttc atcgtcggtg gcggtggcga
    11161 cccgttggag gctgccgaat tgcttcagaa gccgcttgcg gcgaatggtg ccgatgccgg
    11221 cgatctggtc cagttcggat gacagcaagg ccttgccgcg caaattccgg tgataggtca
    11281 gggcgaagcg atgggcttcg tcgcgaatgc gctggaccag gtgggtagcc ggggaggtcg
    11341 gccgcaggac gatggggttt ttccggccgg ggagaaaaat ccgttcttct ttctccccgc
    11401 gggccttggc gaggcccatg atcggaatct ggtcctgtcc gacctgtttc aatccttcga
    11461 gcgcagcgct caactggcct aacccgccat cgatgagaat caggtccgga cgggcgagat
    11521 cttcggtcgc gccgtaccgc cgcatcacgg cttcctgcat gctcgcaaag tcgttggctc
    11581 cctccactgt ctgaatcctg aatttgcgat agtcggattt cttggcctga ccgtcttccc
    11641 agacgaccag cgaggcgacc gattgattgc ccaagatgtt cgaaatgtcg aagccttcaa
    11701 tccggcgagg gaccgtgtcg aggcgaagca ggcgtttgag ttcagccgtc gcctgccggt
    11761 ccagtgcctc attgcggaga tgatcggcaa tggccgccgc ggcattctct tcggcgagga
    11821 gaaccagttg atgcttggtg cctcgctcgg gggccaggat gcgcacgctg tcgccgcgtt
    11881 tctcgctcag ccactgttcg atcagcgggg cctcgtcgag agtcgtgggc accagcagtt
    11941 ccttgggcgg ctgcccttcc ttgttgtaga actgctcgat ggccgagcgc acgagttcct
    12001 cgtcgctcga gtcggtggat tggggccaga agaaatcctt tcgtccgatc aacaggcctc
    12061 cgcgcacgaa caggagctgg agatccgccg cggtgccctg acgggcgagt ccgattacat
    12121 cctggtcggt tgtgctgacc tgagtgatgc gttgtttttc cagcgtacgt tcgacattaa
    12181 acagtcgatc ccgcagccgg gcggcctcct cgaacgcttc ccgttccgcc gcccgctcca
    12241 tgtcctcacg caggctgtcc agcagttccc ggtcccgccc ttcgagaaac tggcgaacct
    12301 gtttgacgat ctgatggtat tcgtcccgcg actggttacc ggtgcaggga gccatgcagc
    12361 gtttgatctc gaactcgatg caggcccggt cggcccggcc gtcgatctcg atttcgcagg
    12421 tggccaaggg aaacgctttg cggatcacct tcagtgtttc gcggagcgcc ccggccgggg
    12481 tgtaaggacc gtagtagagc gctccgtctt tttgcacccg ccggacgatg gagaggcgtg
    12541 gaaagtgttc tttgatcggc aggcgcagat aggggtactg cttgtcgtca cgcaggacga
    12601 tattgaagcg cggccggtgg cgcttgatca gattgctttc gaggatcagg gcttccagct
    12661 cggagcgggt gacgatggtt tcgaggtcgg caatctcgtt gacgagcaga gcggtctttg
    12721 gtgtctggtc gctgcctctc tggaaatagg agcggacccg gtccgccaac acggcggctt
    12781 ttccgacgta gaggatctcc cgcttcgcgt tccgaaagag gtagcagccc ggttgcccgg
    12841 gcaggtgatc gagcttggat tggagcacgt ctgtcgtcat aggtcatcca tcaggcgtaa
    12901 ggataacagg aagggttcct gtatttcctc acgagtgacc attgacggtt gacgagaagt
    12961 tatttgagtc cgcgcacaaa cgacggactc agagcgcctc gggcgacctc tgcgaccggg
    13021 cctttcatcg tgaggctgaa gtcctgggcg acttcgatgt tgagcgtgcc gccgggcatc
    13081 ttcactgtga ccggtccctt caccagtcca agacgaacgg cggcgctggc ggcggcgcag
    13141 gaggacgatc cggaggcctg cgtttcaccg gcgccgcgtt cccagatcag aataaagatg
    13201 tctttcgggc cggtgggcac cgcaagttgg acgttggtcc gcttcgggaa gagggaatgg
    13261 ttttcaagcg caggccccag cgtgaggagg tcctctctcg tccaggcttc gccaggttgt
    13321 ttgaaaatta cgcagtgggg attgccgacg ctgacgccgg tgaattgcaa cgaccgcccg
    13381 gccgcctcga tgggctgctg gatcagttca tcgatcgtga gtgcgcaggg gagggcgctc
    13441 ggttgaaacg tcgcccggcc catttcgacc gtagcggcag cggcatcgcc gtggcggtcg
    13501 agatggaggg caatggagac taggcccccc ttggtttcga cggtgaaggc tcgctttttt
    13561 gttttgccgg tggcatgcag gtagcgggcg aagatgcgca ggccattacc ggatttttcc
    13621 gcctcgctgc cgtccggatt gtagatgcgc aggccaaagt ctgctttctt cgaggggacc
    13681 agcgccagga tcccgtcgct gcccagtccc caatggcgat cgcaaatgcc gcggattttt
    13741 ccgggggtga gtttaaacgt cagttccgtg gggtccatca ccacatagtc gttaccgagt
    13801 ccgtgtccgc ggaaaaaccc gttcttcatc tgcgcgctcc ttctcgtcgt ttgtcgcttg
    13861 tgaaacgtga agcgagaaac aatacgtgga cggattcgga ttcgtcaatg gggtcagccc
    13921 gtgggtgagg aaagggggat gcgtgcactt gtctgtgtcg gacgagcgac gcttcacgaa
    13981 cgacgcgtct tacaccagcg ccgtgcccgg cggcctctcg atgagttcca cttcataacc
    14041 gtccggcgcg tcgataaaga tgaagcgact gccggaggag gttttggtcg ggccgtcggt
    14101 gattctgatc tgtttggatt ccagcgcccg gatcgtgtcg tccaggttct cgacctgaaa
    14161 ggcgaggtgc accaggtctt cctgcacggt caccggtccg ctggcgggaa agctgcacag
    14221 ttcgatgagt tcctcgctgt taggtacttt gaggaacgcg aggtgcgacc cacgcggcga
    14281 gaccttgcgc tccagtacct ccagccccag cacgttctgg taaaaccgga tggtctgatc
    14341 catgtcgctg actcgcatgc gggtatgtag cagctttgtg actttcatat gaggagctta
    14401 tagcagatgg cgtatagcag atggcaagag aagaaatgag aagtgctgct ctgttctctt
    14461 gccagaggct ttggccgtta gctctttcgt gccggccccg tcgtcttgcg cagcagaatt
    14521 tcggcgtagc cggggatgag aaagttcggc atgggaccga tttccttata gccttgcttg
    14581 cggtagaagg cccgggctcc atcgttaaag tccgacacgc aggcaaacat attgttggcc
    14641 cgcgcgaacg tcagggactc gacgtgggtc aagagccggc tgcccaatcc ttgccctacg
    14701 gccgacgggg agattccgag cagctccaga taatccccga acaggaattt cctgcgcacg
    14761 atggcaatgc ccaacacgtt cccttcgatc tccagcacaa atgtgtctcg ccctgcaggc
    14821 agcggcgcaa agatgcggtt ccaatcgacg gccgtgaagc cgagccgttt ccaggggtcg
    14881 gagccggtga ggatctcgat caccggatcg cgatcctccg ctcgcatgtc gcgaattatg
    14941 gtgcgggtaa gactgtcgat tggttgcatc gtaagagatc cgacacggtc atcggggtca
    15001 ggcctttggc cggcaacagg gtagcggcca gggtttcggt cacttcacgg gtatgtttgc
    15061 ctttgccgtt ggcatgcatg acgatgacac tgcctggttt catacgtttc tgaacacgag
    15121 ccacgatcgc ttctgcggtc agtgtcgggt cgggatcgcc ggattcgata ttccacagga
    15181 tgaacttgag cccgagggct ttcacggctt cgaccgtgtg gtcgttatat tctccatagg
    15241 gcggccggaa cagggcggcg gggcgaccgt acttggtctt gagcaggcgc accggtccca
    15301 taatttcctg ttgctgttcc tctacctcgt gcatcggcaa atgggcatgt acctcgccgt
    15361 gggtaccgat ttcgaaaaac ggcacggcga gcagcttctt gacctcggca tcgtgcttgg
    15421 ccatccagcg gccggacatg aaaaacgtcg ccggcacctt atgctcgatg aggaagtcga
    15481 tcagctccca gtcgaacccc gacgccgtgc gcacgggaca caggtcatac gtcagggcca
    15541 cggcggggca ggaggccggt ccggccttga tgacctgcgc ttccgctgaa ggagcctgaa
    15601 ggatcaggag caatccgccg atcaccgaca gtctgaccca gaactgcttg ccacgcatgg
    15661 gtgtagtata cccgaaggca cgaggcggcg atagttgacg ctctgtccgg gtggctgcta
    15721 cggtgaaatt atgcagggcc cagactggaa aatcggccgg atgttcggga ttcccatcca
    15781 cgtgcacgcc tcgtggctct ttgttttttt ctttgtgacc tggtcgctgg cgacgggcta
    15841 tttgcccgac gtgctgcccg gtctcaccga gccgcgatat tgggcgatgg gcggtgtggc
    15901 ggccatcctc ctgtttgcgt ctgtcctgct gcacgagctc ggccattccc tcgtggccct
    15961 tcgttatcgg atcccgatca gtcagatcac cctgtttatc ttcgggggtg tggcgcagat
    16021 gcgaaaggaa ccgccgcacc cccgggcaga gttcctgatt gcgattgccg gcccgatcgt
    16081 gagctttctt ctcgcgggtc tgtgcatcgg tctggtcgcg ttgttggagt tccttccgac
    16141 cggatcatcc gtgcacgggt tggtggcgtt ggggacgctg ctcgggatgg tcaacactca
    16201 gctgggtttg tttaatctgc tcccgggctt tccgctggac ggaggtcgcg ccttgcgagc
    16261 cgggttgtgg gcctggagta aggactacta tcgcgcgaca agccaggccg cattggtcgg
    16321 attgttgttc ggcgtgagtt tcggcctttt cggggccttc ctccttgtcg gggctctttc
    16381 cggtaccgtc tccggcgctc tggcgagcag tgggggctgg atcgtgttgc tgggggcgtt
    16441 tttgttcgcc gccgcgcgtg ggagccggaa gcaggccgcc atccgcgggt cactggcgtc
    16501 ggtgccggtc cgcgagttga tggtcagcaa tgtcgtggcc ttatctcccg acaccacgtt
    16561 agaagaggcc gtgaaccagt attttctccc ctacggctac ggaggattcc ctgtggtgca
    16621 ggacggcagg ttgctgggcg tggtgggggt acgcgatgtg cagacagtga acaattcact
    16681 ctgggcattc cgccgggtgg ccgatatcat gcaaacctcc agcggcgaca tggtggtgtc
    16741 gccggatctc tcggcgatgc aggcgttgga gcagatgttg gcgctggggg ccgaacggct
    16801 cgtggtcgtt caggacgggc aactcctggg cctggtgacg cgcgcttcca tcgggcattt
    16861 catcgagcag cgacatcctt ccgggatggg atcctcctcg taacaggatc agaacgcgtc
    16921 cacacgcggt cttccttgct ccccaattct cctacgcatc agacaacttc tgcccgatca
    16981 ggagaggctt ctttgctgta cgacggccgt ctgcattggc attcatgggt cacgactgcg
    17041 ccgaggagca ccagcgccga ggaatagtag acccagagca tgaggagcac cacttcgagc
    17101 aatgacccgt acagttgagt gtagacggcg gcatcttcca gataggtcac gaacagggct
    17161 ttggcggaca cccataggag gctgaacacc gcccctccga tcgtcgcctc ccgccacgag
    17221 ggccgctgat gcggaagaaa tcgatagaga caggtgacgg aggcgaacgc gagcaggaac
    17281 gggagcgaat aggcgatcag aaaatcatgg gcggcgatgg cgaccaggtt catgcccagg
    17341 aagcggggcg cataggccgt caagagttcg atcgcctggg tggcgacgaa ggagatcaac
    17401 gtcaagaaac ccaacatcca gatcaacgcg accgccacca gcgtggaaat cagcggatgc
    17461 cgcttggccg ccgtgccgaa taccgtgttc atggcgtagt ccaattcata gaataccaac
    17521 gcgctgaacc agccgaacgc gagaaacacc gcccaccgga cttcttcctg ttccgccacg
    17581 cgtcgaatat ccctggccag gcgttctccc atgctgggaa gaaagccttt cagaaacccc
    17641 aggagaaatt gatacccgat cacatcctgg ctgacgatga aactgatgcc gtagagcagc
    17701 aggaacacca tggggaaaag cgacagcagg gagaagaagg ccagcgaggc ggccaagctg
    17761 agacagcctc gccgtcgaaa ggtgtcgaag acattggaga gaaacgacag ttcagtcatg
    17821 ccatcctggc tggtgtggag tcggtgtgct gcgacacatg gcccggtccg gggcgcggta
    17881 tgaggaaggc cggcgcgacg tcgtcgcagg aatgcggacg ccaccgcaat gaggaacggt
    17941 tcgagatgct acttcaagag cggcgcggcc tggctggcca acgaccacag gccattgggg
    18001 aacagaccca gaagcaccac gccggcgagg gcgcaggcca gaaccaatga cagggccgga
    18061 gaggtttcca gtcgtgaatg ggtggcaacg gaaccctcgg cctcgcgcat atacatgacc
    18121 atcacgacgc gcaggtagta gaaggctgaa attgcggcga agatgacggc cacgatggcc
    18181 agccatgtca tgcccccgtt caccgcggcc ataaaaatgt agaacttgcc gatgaagcct
    18241 gcggtcgggg gaatgccggc caatgacacc atgaacacca gcatgaaaaa cgcggcgagc
    18301 ggttcgcgtt tggccagccc ggcataatct tcgatctgct cgctctcctg cccttctttg
    18361 cgcaacatgc cgatcagggc aaatgctccg agcgtcatga acgaatagat ggcgatatac
    18421 agcaacacgc tcgcgaaact agtcgaggct ccttcgccgc tgtggccgcc tgccaccacg
    18481 actccgatca gggcataccc ggcatgggcg atgctggaat aggccagcat gcgtttaatg
    18541 ttggtctgga cgatcgccac gaggttgccc aacgcgaggg tcgccaggca gatcacggta
    18601 aacaagaccg accaatcgga gctggctgct cccaggccct ccacgaacac ccggaggaag
    18661 gcgccgaaac tggcggcttt cgaggcgacg gccataaacg ccgtcacgga ggtcggggcc
    18721 ccctgataga cgtccggggt ccacatgtga aagggcacta cggccatttt gaatccgaat
    18781 ccgaccgcca gcagcaccag cgccatggag aggagcggat ctccggtgcc atgtgtggtg
    18841 atcgcggctg caatggccga caggcctgtg ctgccggcca ggccgaacaa cagcgaaatg
    18901 ccgtacaaca aaattccgga cgagaaggcg cccagtacga aatatttggc cgacgcctcc
    18961 aacgatcgcg cttctgtccg cttgagtccg gccatcacat agagcgagag cgacatcagc
    19021 tcggttccga ggtagatggt caacagatcg gcgccggaca ccatgaccat cattccggcc
    19081 agagcgagca ggatgaagcc gtagtactcc ccgatactca ggcgttcggc tttgagatac
    19141 gccagggaga gcagcacggt gagtccggtg acgatgtaca gcaacaattt ccagaatgcc
    19201 gcataggcat cgatcacgac catgccgctg aacgccgaca cacgacccgt catctgagag
    19261 gaggtgaggc cgatgcacat ggccagcgca cctaatgtca gccaggcgag gacgtctttc
    19321 ttggaggctt caaggatggg atccagcgcc agaatgagac aggccgtccc gatgacgatg
    19381 agttccggaa ggatcgtgag caggtcctgg agtggaaagg tcatcgtgcc gctgcctgtt
    19441 cagaagatga gggagaaagg attgccgcga tcgcggccgt cggcttgcct gcggtgaccg
    19501 gagcctgctg ctgctcgacc gccgccgtag gattcttcgg accgccaagc agctgcgtca
    19561 cgctggcatg catacggctc aacaacgggt ttggaaaaat tcccagcgcg aacacgagta
    19621 cgatcaacgg gaccagtgtc gccgtctcgc gggcgttcag gtccagcagg tgcgcgcgat
    19681 gggcggcaga cggggtcccg aagaccaccc gctgcatcag ccagaggatg taggctgcgg
    19741 ccaggatcac ccccagtgcg gccagggccg tggcaaattg actccagaca aacgtgccgg
    19801 ccaggacgag aaattcgccg acgaaactgt tggtgcccgg aagccccaag gacgacaggg
    19861 cgaaaatcat caggaagatg gcataacgag gcatcggcca ggccaggccc gtattgtcct
    19921 gaatctggcg gctgtgggtg cgttcgtaga tgattccgac gcacaagaag aggcccccgg
    19981 tggtgatgcc gtgattcacc atttgcatga ccgccccttc gatgccttgg atgttcaaga
    20041 cgaagatccc cagtgtaacg aagcccatgt ggctgacgct ggagtaggcg atgagtttct
    20101 tgatgtccgt ctgggccagg gccatgtagg cgccgtaaat gatggcgatg atcgacagcg
    20161 cgatcatcgg cttggtgaag ctcacggtcg cgtccggcaa catcggcagg gtgaaacgaa
    20221 ggaagccgta cgtgcccatt ttcaacaaga cgctggcgag aatcacgctg ccagcggtcg
    20281 gcgcctccac gtgcgcatcg ggcaaccagg tatggaaggg aaacatcggc accttcaccg
    20341 cgaaggcggc gaagaaggcg aggaacaacc acatttggag cgaggcgccg taggtgccgc
    20401 ggctcaacgc caggatgtcg aaggtatgtc cgccctggaa gtacagggcg aggatcgcaa
    20461 ccaggagcaa cacgctgccg gccagggtgt acaggaaaaa cttgatcgcg gcatagaggc
    20521 ggttgggacc gccccagacg ccgatgagca gatacatcgg gatcaacatg gcttcccaga
    20581 acacgtaaaa cagcacgaag tccagggcca cgaacactcc tagcatggcc gtttccatga
    20641 ccagcagcat cgacatgaac agctggacgc gtttttcaat cgccgtccag gacacggtga
    20701 cgcaaaaggg catgagaaac gtcgtcatca acaccagtgg aaaactgatg ccgtcgagac
    20761 cgaggctgta ttgcaccggt ggagagctga tccaggaggc ccgctccacg aactgcatcc
    20821 cggcggttga ggaatcgaag agccaccaga gcggcaggga gagtgcaaag tcggcgacgg
    20881 tcacgccgag cgccagccag cgggcggact ccgccttgac cagcaggcac aacagggctc
    20941 ctgccaacgg caggaagacg atcaaactca gccaggggaa tgaggtcacg aatgcgtcct
    21001 tatagtgttc gtcgcactag aacagcaaat acaccgtcag aatcaccacg gcgcccagcg
    21061 tcatgccgag cgcatagtgc tgcgtttgtc cactctgtgt caggcggatg agccagccgc
    21121 cccaggcaat ggcgcgagcg actccgttga ccgctccgtc aataatcgcg acatcaacat
    21181 gtttccagag cccctgcgcc gccgataggg tcgggcggac cagcgagcgg tcgtaggctt
    21241 catcgatata ccacttgtga agcgacaact cataggctgc gcgccactgc tgcgcgagcc
    21301 ggtccggcaa gccgggattc agcacataca gatagtaggc cgccccgatt cccgtgagcc
    21361 ccatcaacgt ggcgactgcc atgatcccgt aggcggctga tccttcgtga tgcgcggctg
    21421 ccccgtcccc atggaagacc ggttccagga acgctggaat gcccagatac ccggcaacga
    21481 tgctcaggac agccagtacc agaaggggag cggtcatcgt ggtcgagggc tcgtggacat
    21541 ggcctgcgtg atgcggatcg acgcgggact ttccccagaa ggtgacaaag accagacgga
    21601 aactatagaa ggcggtcaat cctgccgtca gcagcccaca gatcgtcagc acctggccca
    21661 agggaccgga tgaccaggaa gagaccagca ggtcgtcctt gctgaagaac ccggcggtga
    21721 gcgggaatcc tgccagcgca agtgagccca ccaggaatgt ccaataggtg actgggagtt
    21781 tatctttcaa accgcccatg tggcgcatgt cctgttcatg gtgcagggcg atgatgaccg
    21841 acccgcaacc gaggaacagg agcgccttga aggcgccgtg ggtgagcagg tggtacatgc
    21901 cggcgctgta ggcgcccaag ccgcaggcca tcaccatgta gcccagctga ctcatcgtcg
    21961 aataggccac cacgcgtttg atgtcggtct gggtcagggc gatggtggcg cccagcatca
    22021 tggtcagccc gccgaccagc gccacgacgg tcatggccgt gggagatagg ttatacagcg
    22081 gcgagaggcg cgcgaccatg aagacaccgg cggtgaccat ggtggcggcg tggatcaagg
    22141 ccgagatcgg cgtcggacct tccatcgcat cggggagcca gacgtgcagc gggacctgcg
    22201 cggatttacc caccgctccg gtaaagagca ggaggcaaat catggtcatg accgagacat
    22261 cccaggtgcc gccgaagggg ccgaggagat tcgtggtctt ggaagccagg tcctgcgcgt
    22321 gcgcgaacac ggtggcgtaa tcgagcgagc cgaaggagta ccagacgagg aacaggccga
    22381 ggatgaagcc gaagtctccc acgcgattga ccaggaaggc cttcgtggcg gcggcacaag
    22441 cgctcgcccg ttcataccaa tggccgatca gcaggtagga gcagagtccc accgcttccc
    22501 agaagacgaa cagctggagc agattgtccg ccatcaccag catgagcatg gagaaggtga
    22561 acagggcgat ataggcaaag aagcgggcgt agcccggttc gccatgcatg tagccgatcg
    22621 tgtagatgtg caccagcgtg ctgacggtgg tgaccagaat caacatgacg gcggtgaggc
    22681 gatcgatgga aatcccgatg tgaatgtcca ggttgccgga ggtcagccag gtatagaggg
    22741 gcacgttgat ggcctggccc gtggccacgt catgcaacgc caggagtgag agcagaaacg
    22801 aacccaccat ggccggaacc gccaccagat gcgcacggtc cttgatccac tgttcgccga
    22861 taccgacgat cagaaaggcg aacaacggca gaaatggaat caacgcatac agcatggtcg
    22921 gcgtagtcgg tctaacgttt caataggttc aggtcgtcga cgtaaatgga tgaattcgac
    22981 cggtgcaggg cgatgatgat ggccaggccc acggccactt ccgccgccgc gacggtcaag
    23041 gcgaaaaaga cgaacacctg ccccgccaca ttgtgaaagt attcggaaaa ggccacgaag
    23101 ttgatgttcg tggcattcag catcaattca accgacagca ggatcacgat gatgttgcgc
    23161 cggatcaaca cgcccaccac acccgtgaga aacacgaacc cgctcaggat cagatagtag
    23221 gataagggta cagccatggg attaccggtt gttactgcac gggttcattg atgtctttct
    23281 tcgccaggat gatggccccg atcatggcca ccagcaggat caacgacgcg acctcgaacg
    23341 ggaacaaata ggtcgagtac aaggcttccc cgatcatttc cgtattgcct ggtcctgcga
    23401 cgggatcggc tgcagctgcc ggagccatcc ccctggtctg cgacttgatg aacagcagga
    23461 tcacttcggt acacagcacc acccctagca atcccgccac cgggagctgg ttgtggtagc
    23521 gctcctccga cttgatgctc agcagcatga ctacgaagag gtagagcacc aggattgcgc
    23581 cggcatacac gacgatctgc accgcggcca gaaactccgc atgcagggtg atatagagcc
    23641 ccgccacgtg aaaaaacatg atcagcaggg acaacgcgct atagaccggg tttcgcaacg
    23701 cgactaccag cagggaggtg gctgcgatca ctgcggcgaa atagaagaag aaaatgtggt
    23761 ccatgcgtcc gtcgtctctt ccgaatcaga tgaaggtcct gccccctgtt gcccttgcgc
    23821 cctgcccggt ggcagcctgt tccgcctcgg tcgtctcggt gtggggggcc agcacggagg
    23881 acgaggcggg tggcctagtt ctccttttgc gggagatgcg tgaaggtgac gttgaaaaac
    23941 gccacgttcg gatgctgcag ctcgaggcgt ttttcccgga cgggaaatga acggtcgccg
    24001 atggcgagca gttgttgctt gttgaggtgc agctggcgct tatcgtagac ggcccattcg
    24061 aactcgcgag tcatgcccag ggcatccacg ggacaggcgt cgacgcagag accgcagaac
    24121 agacagcgcg tcatatccat gtagtattcc ttcgaatacc gtttggtcgg ttccccgggg
    24181 acttctccgc tgacgacccg gatgacccgc gagggacagg ccgcttcgca cagatcgcag
    24241 cccacgcatt tttccgtccc gtcgtcatac cgcagcaagg ccagcatgcc ccggtagttg
    24301 tcgggcaaca ggcgcttctc atgcgggtat tgcagcgtga tcggcttgta gttgagcagg
    24361 tgtgtcagtg tcgccttcat gcccaccagg agttcgtaga aggtcagcgt cttcagccaa
    24421 tcgctgaacg acggtttccg cttgggttgc gttgtgacgg cgacgttcat ttcgctccgt
    24481 taccgcggga agaaataggc ggcaatggcc gtcaggacga tgttgcccag tgcgatcggg
    24541 agcatcactt tccagccgaa ccgcatcaat tggtcatacc gcaggcgggg cagcgtcgcc
    24601 cgcaaccaga agaacaggaa caggaagaag tagaccttcg cggcaaacca gacgacgttt
    24661 tcaatccagg cgaggctgtc caggccgatg tacccgagga tggtgcccgg gtagggcgcg
    24721 ttccatccgc cgaggaacag ggccgccgcg acgcaggaca ccagaatcat gttggcgtat
    24781 tccgcgatga agaagaaggc gaagcgcatg ccgctgtatt cggtgaagaa gcccgcgacg
    24841 agttcgcttt cagcttcggg caggtcgaac ggcacccggt tggtttcagc gaccgctgaa
    24901 atgacgtaga ccacgaaggc gaaaatctgc gggaagggca tggcgatgac gaaccagttc
    24961 cagaacccgc cggcctgcgc ttccgtgatc ttcaccaggc tgagcgagcc ggacaggaga
    25021 atgactccga cgatcgccag gccaacgcac aattcgtagc tgatcacctg tgcggcggag
    25081 cggagtccgc cgaggaggga gtatttactg ttcgaggacc agccacccag aatgattccg
    25141 taagcgccga tcgaggcaaa ggccaggatg tacaggatgc cgatgttgat gtcgctgatc
    25201 acgaacggct tgatctgcat cccgaacaat tcgatggtca ggctcgggcc aaagggaatg
    25261 acggcgaatc cgatcatggc cggcaccagg gccaggatcg gtgcaagggt aaacaggaac
    25321 ttattggccc ccgccgggat gatgtcttct ttgaagaaga gtttgagtcc gtcggcgatc
    25381 ggctgcagca ccccgtaagg gccgacttcc atcgggccca tacgatcctg catccagccc
    25441 agaactttcc gttccgcgag ggtgaggacc atgacggtca gcatcaccac tcccattacc
    25501 gcggcgattt gagctaacga caccgccagt cgtaatccga tttccatgac aacaatactc
    25561 ctcgtcccgt cctacgagac cttcgtgaga gacacgtgcg cgagcttaaa ataggggatt
    25621 tgagtccggg gatcaatcgt ccattcagcg agctgtttgg cctcgccgtc gaaatgttcc
    25681 ggaaaccaca gcaggccggc cggcacccgt tcgcgaatct tcaccgttgt ggtgatggcg
    25741 ccgcgtgagt tcgagacctt cacctgtccg ccgtccgcca ggccgagccg agctgcatcc
    25801 gccggattga tggagaggaa cccttcctgc tgcaattgga ggagcccctt ggcgcgtgtg
    25861 gacagcttcc cggagtgaaa cagcgtttgc acgaacatca gcgtgaacgg gccgtcgtta
    25921 gtctgccgag gctggctcac ggcatagcgt gtggccgcgt cgactgcaaa cccttccgtc
    25981 agatatcggt tcaacaccat tgggtcgact ttgggtggcg taggagtcgg tcctaacagt
    26041 ccataaccgg ggatgaccgt ccgaatctct ttcaggattt cccgcgcgtc gccatattca
    26101 agcggcgagc ccatcagcac ggagagcgcc gataacattt cccagtcggg gcggctgtcg
    26161 cccacgggat tgatcgcttg ccgaaccccc tgaacatggc cttcggaatt cgtgaaggtg
    26221 ccgtcctttt ccatatagga gcaggcgggg agcaccacat gggctatcgc cgccgtctcg
    26281 gtgaggaaca attcctgaca gaccagcagg tccaacttcc ccagggcttc tttggcctgg
    26341 gccgcggcgg gcagggtgcc gaccgggttt tccccgacca cgaacatggc cttgagagtg
    26401 cccttgtttg cggcggcaag catctcggtc agcgaggcgc cgggagtgcg cggtaagtct
    26461 tctcgccaca tggctgcgat gcgatcgcgg acgggctggt cgtcgagcgg agcgggccca
    26521 ggaaagaatt cggcgacggc gcccatttcg acggcacctt gatcgttatt ttcttcggcg
    26581 agcggcccga gtccgcagcc cgccttgtcg agttttccaa gcagaatcag caggtccaga
    26641 agattcgtga ccgtcgcgtc tccgccagga tggcgcagca caccgttgcc gaccaggatg
    26701 accagccggt tggctttcgc cagtactgtg gcggcctctg cccactgggc gcggttctgg
    26761 ccggtcgccg cctccagggc ctcccacgag gtgccctgca gagcggaagt cagacgctgc
    26821 acgaaggccg gcgcctgctg cgcgacggcg gcatcgacca ggttgccttc gatgacggcc
    26881 ttgatgacgc ctaacacggt attgccgaat tgcgcgggat gcgtcgggaa atgctgaagg
    26941 gccaggttcg tgatattgct cagggtatcg atggccggct ggagcgtttc cacggtcacg
    27001 agactggcct gccgtttctt gacggcttct ttgaccttga gcccggtgat cgggctggtc
    27061 tcggtgatgt tcgtaccgac caggagcaat gcattcgcgg cgacgatgtc ttcaaaggcg
    27121 atggtccagc gatgggtacc ctgcacgcgt cggagcgcct gcactccgtt gaggtggcca
    27181 taccgcgcgc tgctgtcgat ccggttggtg ccgatcacct ggcgcatgaa cttttggaac
    27241 acatagaggt cttcgttcgt gcaacgggag ctgataagcc caccgaacgc atccgcgccc
    27301 tgcgccagct tcaggcggag cgcctgttcg gcaacatatt ccagcgcgtc ttcccaggtg
    27361 gtctcgacca acttgccttc gcgacggatc aacggatgtg taaggcggtc ggcgtggctg
    27421 ctggcatgga agccgaagaa gccccgggcg cagaggtcgc cgttattccg tccggctccg
    27481 tgtgcggagt tcacctcgat cagttcattg tccttggtct gcacggtgat ccggcagccg
    27541 tcgccgcaga aggtgcagat cgtgtcggcc cgcttgagca tccaggggcg atactcatac
    27601 atggagagcc ggctcgtaat ggcgccgacc ggacagatct gaatacagcc gccgcagaat
    27661 tcgcagtcca gttcatgcgg gccgaagtgt ttgatctcgg tcatggtgcc gcgccccacc
    27721 ggagccagcg ctttcacgtc catgatctca tcgcaatacc ggacgcagcg caaacactgc
    27781 acacagcggt tcatctgcgt ttcgatgagg gggctgaagt actctttctg gaagatgcgt
    27841 ttggtttccg taaaccggct ggtcgtcggg gtgtattgat gcgagaaatc ctggaggtcg
    27901 catttgccgc cctggtcgca gaccgggcaa tccagcggat ggttggccag gatgaattcc
    27961 aggaccgact tgtgggcgtc gtccaccgtg gtggtggcgg tgcgcacggc catgccttcg
    28021 gcgaccggcg tgctgcaggc ggtctggagc ttcggcatct tctcgacttc gaccaaacac
    28081 atccggcagt tggcgtccgg cttcagcttg gggtggtaac aaaaatgcgg aatcatcacc
    28141 ccgacgcgcc gcgccgcttc gatcaccagg gttcccttgg gaaccgcgac cgtcgtgccg
    28201 tcgatagtga gccggactgt ttctggtttt ggatcaggca tagtggccgt cattcgtgaa
    28261 acgtgaaacg tgaaacgccg tgaacggcgg ggagccgtcg cccgtctcgt tgtcgatctt
    28321 ctcgaacgac gagagacgct tcacgagata cgtcaatgtt tggctcccgc cggttccggc
    28381 cgtattaaat ttgccgcctc ggccgcatgg atcatagcga catactcgtg ccgccaatgt
    28441 ttgagcgtgc tcagaatcgg cgatacctcg gcatcgccga aggcgcagac cgttcgaccg
    28501 gcgatgtttt tacacaggtc ggtcagggtt tcaaggtccg ccatctgccc ccgcttgttg
    28561 acgatccggc gcatgatctg cacgagccac gagctgcctt cgcggcaggg actgcacttg
    28621 ccgcaggact cgtgatagaa aaactccatc agccgcaggg ccgcccatac catgtcggtg
    28681 ccctcttcca tgaccgtcac accgccggac cccagcatgg acccggccaa cgcaacggat
    28741 tcgaaatcca gtttgacgtc caggtgatcg ggcgtcagga agggcgcaga ggcgcctccg
    28801 ggaatgaagg ccttcaattt cttgtccgac cgcataccgc cggcctgttc gtagatcagt
    28861 tcgcgaaagg tgatacccat cgggacttcg taattcccgg gcctcgcgac atgcccgctg
    28921 acgcagaaga cgcgtgtgcc ggtgctcttc ggcggtgacc cgatcgaggc gaaccattcg
    28981 gcgccccggt tcaggatgtg cggcagattg gccagggttt cgacattgtt caccacggtc
    29041 ggcttgttgt agagcccgtg ggtggcgggg aacggcggct tgacacgcgg gaggccgcgc
    29101 ttgccttcga gcgattccaa cagcgccgtc tcttcgccgc aaatataggc gcccgctccg
    29161 cggtgtaccc aaatgtcgat cgtggtgttc gagccgaaaa tgttcttgcc gacatatccc
    29221 gcggcgcgcg cgtcattgat ggcctgttcg agaatcttgg cccccaggac aaattccccg
    29281 cgaatataga tgtaggagct ggccgcgccg atggcgtagc aggcgagcaa catgccttcg
    29341 agcaattggt gcgggtcacg ttcgatgagc tgccgatctt tgaaggtacc cggttcgctt
    29401 tcatcggcgt tgcagcagag gtagcgcgga ccctggtagt ccttgggcag gaacccccat
    29461 ttgactccgg tcgggaagcc ggcgccgccg cgcccgcgca agccggactt catgaccacc
    29521 gcagtcacgt ccgcagggct caccttgccc agcaccttcc ggatcgcctg gtagccgccg
    29581 acgcgttcat actccgtcaa cgaacccgta tagccgggtt gcagcatgtt tttcagcaga
    29641 ataggttcgt acttgggcat agcttcagcg tgaaacgaaa aaagtgaaag gtgaaaggtt
    29701 gaggcccgtt atagtgttgc gtcgtctcac gttttacgcc tcacgtttca cggcattcgc
    29761 cggttccggc cacatgaagg ggccggtctt cagcgaacag gtgccggtct gtcgtaggtc
    29821 ggccagaatg cgatccagct tctcttccgt cagccgctcg taatagtcgt cgttgacctg
    29881 catcatcggg ccggttccgc atgccgccag acattccacg atactcaagg taaagagttt
    29941 gtccggcgtc gtctcgcccg gcttgatgcc gagtttcgcg ctgatccagc cgataatcgt
    30001 gtccgagccg acgagcgcgc acatgagcga cttgcagacc tgcaggtgga atgtccccac
    30061 cggcttcaag ttcagcatcg tataaaacgt ggccgtctcg tacacctgcg gcggagtcag
    30121 cctcaagatc ccggcgattt cctgcatcgc cgcttcggtg acgtagccct gttcccgctg
    30181 cgccatgtac agcagaggca gcaatgccga ccgtttcact ggatagcggg acagaatgtc
    30241 ttcgatctct gccttgtgcg tctctttcag catctatcgt gtccctatgc caacaccaca
    30301 atgaatcgtg aggttgtctt cttcctgcgc gcgcccctca tctatcacat tctcccatca
    30361 cgatgtcgta ggttccgaag atggtgatga tgtccgagat caggtagccg cgggccatgt
    30421 ggtcgaaagc gcccatgtga atgaatgagg gggcgcgaat tttcaagcgg tagggtcgcg
    30481 ggcttccgtc gctgacgatg aagaacccga gttctccttt gggcgcttca gtaccgcagt
    30541 aggtttcgcc tttcggcggc ttgaacccct gcgtgaaaat aataaagtgg tgaatcaggc
    30601 tctccatgtc gcgcatgact ttcgccttgg gcgggggaat cacgtgcggc acgtccgcca
    30661 tgatcgggcc ttccggcatc tggtcgaggc attgggcgat gatccgcgcg ctctgccgca
    30721 tctcctccat gcgcacccag tagcgatcgt acgtgtcccc gcgtttgccg accggcactt
    30781 cccattcgac cttgtcgtag actccgtagg gctcgtattt gcgcacatcg taatcgacac
    30841 ccgatcctcg gagcgacggc ccggtgagcc cgaaattgat cgcgtcttcc gccgaaatca
    30901 cggccacgtc tttggtccgt ccgacccaga tccggttgga catgagcagc tggttgtatt
    30961 cgttgacctt ctcggggaac gtctgcagga acgctttcag gcggaccacg agatccgggg
    31021 tgaaatcgct gtccaccccg ccgatgcgat agtagttcaa ggtcagccgc gcgccgcaga
    31081 gcttttcgaa catgtccagc agcacttcgc gttcgcgaaa cgtccagaag aagacggtca
    31141 tggccccgat gtcgagcgcc tgggtgccga gccagaagag gtggccgatg atgcgttgca
    31201 tctcagcgac gatggtgcgg acgtattccg cccgttccgg cacggtgatg tcgagcagct
    31261 tctccaccgc gcggacgtaa gcatagttgt tcgtcatcgc gcagacataa tcgagccggt
    31321 cggtatgcgg gatgacctgc atatagtgca gaccttccgc cagcttctcg acgccgcgat
    31381 gcaagtagcc gagatccggc gtggccttga cgatccgttc accgtccagt tccagcacca
    31441 ctctgagcac gccgtgggta ctggggtgtt gcggccccat gttcaacaac agttcttcac
    31501 ggcgacgcgt gccgccctgg tgctcagcga cgaggtacgc ctgcttctcc tgctccggaa
    31561 cttccccttc cgtctgttct tcgggagctt cgtccaggcg cggaatgaac gggaacgagc
    31621 tgcgccagcc acgaccttcg gtcgggaaat ccttacgtag cggatggccc tcttcgtaat
    31681 cctccggcag gagaatgcgt cgaagatcgg ggtgaccgac gaaggtgatg cccatcaggt
    31741 cgtagacctc acgctccatg aactcggcgc ccttccagat accggtcacg gaggcgatct
    31801 gtggtgcatc ttcagtcacg cgggctttta atcggatgcg tcggcggtgc gcgagcgaga
    31861 ggagttgata gaccacctca aaccgttccg ggtcattcgg gtagtccacc gaacagatat
    31921 cggtgatatg gtcgaacgag gcctccggcg tgtcgtgcag ccaccgcgca acgtcgacga
    31981 ttcgagcggc ggcgacgcgg gccgtgatct cgtttcgggc ggtatccact tccacgccgc
    32041 ggacggcgtc gggaaaggta gtcatgagtc gttcaagcag cggttgcatc ttc
//
LOCUS       DAORRQ010000049        31553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_88_length_31553_cov_88.135215, whole genome shotgun sequence.
ACCESSION   DAORRQ010000049 DAORRQ010000000
VERSION     DAORRQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88_length_31553_cov_88.135215"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2687)
                     /locus_tag="PK224_14705"
     CDS             complement(<1..2687)
                     /locus_tag="PK224_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRB17043.1"
                     /translation="MMQLSRRQFLKVSAGTVAVAAVADKALALTALQPVVEVDNPLGE
                     YPDRSWERVYHDQYRYDSSFTWCCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDL
                     YGNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKQWMDDGSPELTSDAKRKY
                     KFDSRFLDDMVRVSWDTAFTYVAKGLIVIGTRYSGEAGARRLREQGYAPEMIEMMKGA
                     GVRTFKHRAGMPILGMMGKHANTRFNNCVLPLLDSWIRKVNPDQAQGGRYWNNYTWHG
                     DQDPSQPWWNGTQNCDVDLSDMRFTKLNTSWGKNFVENKMPEAHWKLESMERGARLVV
                     ITPEYNPTASRADYWIPVRPETDGALFLGASKIILDENYQDIDFIKGFTDMPLLVRTD
                     TLQYLDPHEVLKDYRVPDFTKSYSGRVQGLTQDQVQRLGGMMVWDLAKGKAVPLHREQ
                     VGLHLAQSGIDPALTGTYRIKLLNGREVDVMPIYQLYTIHLQDYDLDTVHQVNRSPKD
                     LIVRWARDCGTVKPAAIHNGEGVCHYFHMTSMGRAAALVMMLTGNIGKFGTGCHTWSG
                     NYKVGIWQAAPWSGAGASVYLGEDPWNLNLKEDVHGKEIKYRKYYYGEEPGYWNHGDN
                     ALIVNTPKYGRKVFTGKTHMPSPSKVRWVVNVNILNNAKHHYDMVKNVDPNIEMLITQ
                     DIEMTSDVNHADVAFAVNSWMEFTYPEMTATVSNPWVQIWKGGIRPLYDTRNDLDSFA
                     GVAAKLKEITGEQRMSDTYKFVYHNRVDIYVQRILDASSTFFGYSADVMLKSEKGWMV
                     MCRTYPRHPLWEETNESKPHWTRSGRLETYRVEPEAIEYGENFISHREGPECTPYMPN
                     AIMTSNPYVRPEDYGIPVTAQHHDDKTVRNIKLPWSEIK"
     gene            complement(3140..4660)
                     /locus_tag="PK224_14710"
     CDS             complement(3140..4660)
                     /locus_tag="PK224_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB17044.1"
                     /translation="MHNAALNYRTLLSVTNVLNSQRNRQSLFRAVTDQLAKVVRWERA
                     GITVYDLKSDAFRFYAVETNLPKVVLRSDAMIPRAHSAVGWVYDHHQVHVRPHLQRER
                     LFIEDESYLQEGLGRMINLPMVVQEACLGTLNIGSVEAGHPDPADVDFLQQVATQIGF
                     AIAHVNAYEEINRLKEQLARENVYLAEELKANQDFGIVVGRSQAFEQVLTLARQAAPT
                     GATVMVTGETGTGKEVLARAIHERSLRRDRAFVRLNCAALPSGLIESELFGHERGAFT
                     GADHQRIGRFELADGGTLFLDEVGEMPLEAQAKLLRVLQDGLVDRVGGANAIPVDVRV
                     IAATNADLHRAIQQGRFRSDLYYRLNVFPIHMPPLRERPEDIPILARHFLRVHSQRLR
                     RHCEDFDGPSMERLVQYAWPGNVRELENIVERALILCHERLLRIDPAMVNVGVSPEAG
                     PRRTLHDEERHHILQTLTLLDWRIEGLGGAADQLGLAPSTLRSRMQKLAIRRPSRM"
     gene            complement(4693..4830)
                     /locus_tag="PK224_14715"
     CDS             complement(4693..4830)
                     /locus_tag="PK224_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17045.1"
                     /translation="MGPAYDAHSAQVTTATEAYLQFSAVRAFASPDDVLLQRTGSVSR
                     S"
     gene            complement(4945..6492)
                     /locus_tag="PK224_14720"
     CDS             complement(4945..6492)
                     /locus_tag="PK224_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRB17046.1"
                     /translation="MTTTTADMSQHPQSSGGPPPAGPYRIHPKAIRARRNRRLLVVAG
                     LVLVATVAYLVYWYTHDRFWVRTDNAYVTGNLVPVAAQASGIVTQVLAEETQFVNRGD
                     LMIKLDEHQAYAALGRARGRLGEEVRRIASLFINRKQLAEKLRSRRARLELAEHDMDR
                     YQRAAPSGAISKQILQNTADKIASLEAEVRETQAELDSLDAQIGGTTVMAHPAVDLAK
                     HQLIEAHLEYARQQIRAPISGYVAKRKAQVGDRVQPGVLLMTIVPLDHLWVEANLRET
                     ELQHVRPGQPALVNVSLYGSKQTFHGTVEGLVPGSGSAFALLPPDNSTGNFIHIVERV
                     PVRIALPADELREHPIRPGLSTVTSINITESGQSVWTSLATPSTAEYETDVYADELPM
                     AESLAKEVIATNLVVGDRGASIDSLLDEEEEIQRMTSRNGDMRMSPEARIPVDRDRMR
                     THRDPVPSAFVPRRSPDLGVTTTPLAPSIGPGSGSLGPEAGRSGSRLRPGFETDEGRR
                     ALGAGRH"
     gene            complement(6497..8005)
                     /locus_tag="PK224_14725"
     CDS             complement(6497..8005)
                     /locus_tag="PK224_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRB17047.1"
                     /translation="MILPVTMRRRLQLALGIGCALFFGGCAWIPKGDQPAQYLEAPEM
                     TETLAEVTSRLQNWPEDRWWEQFGNPELNGLIEQSLTDNPGLKHAAARLRQATSLVKV
                     EGARLLPFLEADASLTYERISQHGVFAALNPEVAGIRIMYGIINPLSFRYEFDFWGKN
                     RAMLEAALGHAAAEEAEAAEVRLRLTTGIARAYFRGQALQQQLTIVKTIVGIRRELKT
                     LAETRFRLGLDNDQPVKIAMADYEAAFKRQAAIRDQLDVQRHLLARLVGKGPDEGAHL
                     FAKPKVIVPNQIAAPDHLSLGLLVHRPDLAAALYRAHAASRLVRVATTQFYPTIDLTG
                     FVGFNALTLAKGTDKLANFLFSGQSFSYGLAPGLRMPWFEGGRLRGELGAQRAEYDAA
                     VELYNDTLLEAMREVADSLSAWQTTNEIMASHKRLVASLGDDWRLAKVRLVNGLDDDR
                     EVLRHRYPVLEQEYALRALESDQLVAAVDLIESLGGGYHNPDIEKRPKQDPS"
     gene            complement(8002..9600)
                     /locus_tag="PK224_14730"
     CDS             complement(8002..9600)
                     /locus_tag="PK224_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRB17048.1"
                     /translation="MAAVYLRRLHGWRFVLFNAVLGLSHIVVLFNAGSYIALLPHVSG
                     DLGGVLPSFLTWAQTDFMVGLALGFPLARYLSGRIGDYRLLIGAFMVYSVASYLCGDS
                     QTLAQFVPARIVQGIAGGITLPIAQSMLLNEYPKRLKAVALTVWGLFSITPFTIGMPV
                     GGYIAYMLGWRFLFYLDFGLTLVIVGTLAALLYGRGFRRRYARFDLVGFVLLAVLLLG
                     LQTLLNMGNDFDWLDSPFLQAIAVVLLVACPCFVIWELGERHPILDLRLFLHRNFLIG
                     VISLTLGFFSIQGLLSLLIVQIQYLLGYSSKLAGLALLPMVLLGAPVIAVMHYLCKYV
                     DARWLVCFNSLGFALTFYWIGLYDDPHSYEELFWPMVVEGIFLGSFFTPMTVLLLHGL
                     SGPLVTRAAEIANLLRVAAGAFGITFQGIVLFRRTHFHQLHLADHFGGRQSISFDPMG
                     QLAAKLSAAGLTPAEVQARLAAIIKQAANLLSMSDAFLVASYLFIGLGVLVWFAHPTH
                     ILLSLNREEELREMRAEEMMEEVP"
     gene            complement(9618..10556)
                     /locus_tag="PK224_14735"
     CDS             complement(9618..10556)
                     /locus_tag="PK224_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRB17049.1"
                     /translation="MTVPCIARRVMVGEPKKEDAMDYVKPYGVVDSMLAGGALKLSLP
                     PRQLVLRGMLAGAYLGIGTSMAVTVAVETGYWIIGSLLFPFGLALAILLGAEIITGSF
                     ALLPVAVADGQKNAGLPHVLANMGWVFLGNLLGSVLYAGLFAIALTTAGDAHINAVGT
                     KLIAVAEAKTNYYVSHGSSGLLAAFTKGMLCNWMVSLAVVAAYMSTSFSGKILAIWGP
                     TLLFFSQGFEHAVVNMFLIPVGMMLGGNISISTWWLWNQIPVTLGNLVGGMLFTGLAI
                     YAAHRVVAPAAQPVPAAAPVGHPNSQAKQPGYSPSF"
     gene            10904..12859
                     /locus_tag="PK224_14740"
     CDS             10904..12859
                     /locus_tag="PK224_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB17050.1"
                     /translation="MSDLLSNLPIARKLFLASVIPALTVLLLSVLTYRTVTTFSDDEG
                     QLNDIYYSQRLASEYLRLVVDLETGFRGFVLTSQEHYLFPYRTAQDHVLNIGRTLEAQ
                     VSAYDDQRALITSVQQLVRQFIDEKETLIDAVKAEHPDEARLYIEEGKGRTLMLTIRQ
                     DMGRFEHLAQTALNTKLAKLAQDRDTMLMTILGGGLFALICMVAALHLIAHSITTPLE
                     RLATAVMAADTHPIPHVPVMTRTDEIGNLSRVMHAMSSAIHSHIAALQLSEANLRQLN
                     QDLAGSEAKYRSIVDHAPFGIFTTRGMTLVFSNRYNSLLAGLDPSEEKDPDAVRRAIH
                     PEDRERVIEEFAQAVTEGQPYETVFRFLHPDGTVRKVLSRRIPIRDHSGEVVMYQGFN
                     VDITALDLMQSRLRRAERLATLGQVAAGIAHEIRNPLVGIGSTTSLLLDDTDPADARR
                     PDLAVILQETKRLDRIVNQIIDYARPREIVAFAFDMAQLVQEVIKVLDEPLTRKQATI
                     RLSAPDAPYTIQADRDQLKQVLLNVLQNAIEASPSDGTITVTLVQQDRGVEPGLEVAV
                     ADRGTGISPTHLPHVFEPFFTSGKPQGTGLGLAICRNILEAHGGEIALDSEMGRGTTV
                     RVWAPLRQQPPRMQEEEQHAGHDLRHG"
     gene            12831..14222
                     /locus_tag="PK224_14745"
     CDS             12831..14222
                     /locus_tag="PK224_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB17051.1"
                     /translation="MQATIYVTDDEPAIRSAIVKRLSRRHHTVTGFESGEDVIRAVTQ
                     HAPDLILLDLKMPGIGGVETLRQLRPIAPQTLIIMLTAYGTVEDAVEAMRLGAYDFLI
                     KSVDLSGVDPVVDRAIEFLTLRHRVEFSLEDQNSAYALSNIDARSPAMQLLLGQVRDV
                     AENAKSTVLLQGETGTGKEFLARVIHCNSPRASGPFVAVNCTAIPRELFESELFGHER
                     GAFTGAHQRKRGLLEKAEGGTLFLDEIGDLDLAMQAKLLRVLQERTFRRVGGTEDLSV
                     DFRLMTATNRDLKKDTARGSFREDLFFRLNVVTFELPPLRVRTEDILPLSMQAMLRFG
                     KEFGKEVLDIEPDAQELLQRYSYPGNIRELQNIIERAMILCHDKTLTAGCLPRELREQ
                     APHIAVAMAQGEHPSLRIEMVLGQQTLADIESALIEEVVRLSDHNKTLAAKHLGLTRF
                     ALDRRLKKQMEQD"
     gene            14305..15447
                     /locus_tag="PK224_14750"
     CDS             14305..15447
                     /locus_tag="PK224_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRB17052.1"
                     /translation="MTGKHNSWIAPGIVGLTCGLFALDLFMPLGVAIGALYAGIVLLA
                     SFSPNPRLPLLTAACATGLLIVGAVAGPRLSSIPLWVGVTNASLGLVVVWISAVLLQQ
                     RHHAEIRLRQAKDELEARVAARTRELADVNRTLLHEISEHVETEESLRASEHDLATSR
                     QELRDLAARLLTVQEEERRRISRDLHDDINQRLAMLVVQAESLESSLPPSASDCSKEL
                     RSIQDRLTELSDDVRHLAYQFHPSILDDLGLTVALQRLVDECATRANLQGAFEVSLAP
                     PTVPQTVSTCLYRIAQESLANVMKHARANKVIVSLASTADAITLTVQDDGVGFDTQQL
                     ANSHRGLGLISMAERLRLVRGTVTIDSIPHQGTRLYIKVPHAEVPV"
     gene            15444..16088
                     /locus_tag="PK224_14755"
     CDS             15444..16088
                     /locus_tag="PK224_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17053.1"
                     /translation="MTTPPRVLLADDHTLVLEGFRRIVEQRCEVVGAVEDGRALLDAA
                     TRLRPDLILLDISMPLLNGVDAARQLKKLLPDVNLIFVTMHADPAYVSEAFKAGASAY
                     LLKRSAARELEQAIDSVLKGQYFVTSLLTKDLVTTLSAGQAGLFAQRQELTPRQREVL
                     QLIAEGRTIKEIASLLNISPKTVEFHKAQIIFHLNLRTTAELTKYALAHGLTSA"
     gene            17080..18525
                     /locus_tag="PK224_14760"
     CDS             17080..18525
                     /locus_tag="PK224_14760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB17054.1"
                     /translation="MNYADQRGPMHEPQQQSEAEDGRVYGPQLGSASIPQRSAPWGER
                     LAPALRLDARIAPFALLACLFVVFSSPISSLAGDRATAPPAGPSDPVYSLEAVLNFAL
                     TRNPTVASAEGTIDQNRGQQVAAYTYLNPSVSANSGVGKMRDLGLFDPSVRERVTEFN
                     LTVGQPIEWPSKRAARQRASEAGVAAASAGLAETQLNLVADVKVAFYDLLLAQRALIL
                     AQQNLATVEDVDKAVRTRVRLGESPQFEAIRSGVEVLKANQSLTRAANRVRVNRVMLD
                     TLTAGSLGPTYAIDGQLHRVGPGFGIDILTERALTQHPTIVRLRKSVEQADHSLEFER
                     QARVPNITIGGSYWREIGREAFTGGVTFPTPVWDRRQGEIISAFGSKRKGEAEFLRAR
                     NELIRNVSQHFQDAKTTADMIEVYEKGLLKQADEALRIAKFSFQQGASSLIEVLDAQR
                     VQRQILLDYAQAQFDLSMSLALLERAVGGAI"
     gene            18522..19754
                     /locus_tag="PK224_14765"
     CDS             18522..19754
                     /locus_tag="PK224_14765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17055.1"
                     /translation="MMHHIKHATTRSRSFRWTHNRPFVLMMLAGMFALLALPGCDGPP
                     PNATGANPPPSADKSLVRLTTEEIKSAGIIVQPVTRSEFRTIRDFPGTVEPNEHALAE
                     ITTLVRGRVIDVYADLGREVKGGTLLALLYSSELGMAQSAYLKATAKLNVAERAFRRA
                     ELLLKEKVIGVAELQRREGEMLSLRAELREARDRLLILGLTDEDLRNLDRNHTIRSHV
                     PVVAPFDGRIIARNLTKGEVVETTEKLFVVADLTDVWVTAVIPEKDIPYIRPDQTGTG
                     QSVEVHVAAYPGQAFQGRITYVGDVLDPATRTMRLRLELPNPERKLKPAMYATVRVYS
                     EPEANALLIPESAVQRDRDRQFVFVEREPAIFEARDVKLGSSNGREIKVLDGLLEGES
                     IVTNGAFVLKSELLGEQM"
     gene            19759..22890
                     /locus_tag="PK224_14770"
     CDS             19759..22890
                     /locus_tag="PK224_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRB17056.1"
                     /translation="MLSSLLAFALRQRVLVVVMACILTAGGIYAFRTIAIDAFPDVTT
                     VLVQVVTKVPGMSPAEVERFVTFPMELQLMGAPGLTDIRSFSKVGLSMITVVFKDDID
                     IYLARQVVLERILEIQELLPPGSSSQLVPNTTGLGEVYQFYLEGRHDNDKDFVMSDSD
                     LMERRTIEDWVIRPLLKGLPDVVDVNSLGGFVKQYQVMVEPGLLRKYGLSLHDVFDAV
                     AKNNANAGGNILEKDDEKYVIRGVGLIKTLEDIDHIVVKQVGGTPVYVRDVAEVRIGH
                     AVRHGAAVLNGKHEVITGIVLMLRGGNARDVVQSIKDKISEIHHKRLLPDGLRIIPFY
                     DRIELITAALDTVYKALLEGIVLVVVILFLFLGNVRSALIVTATLIVTPLVTFIIMDQ
                     VGLTANLMSLGGLVIAIGMMVDGSVVVVENVYRHLAEQRDDRLSKTEVILHAVTEVGQ
                     PVIFGILIIILVFLPILTLQGMEGKMFQPLANTIIIALLVSLVLSLTLSPVLCAMALK
                     RGTEEDTFLLRWAKWAYLPTLRWAMGHRVTVLTIALGLLGASLSLFPFLGGEFIPILN
                     EGAISPQTIRYPSIALDRSIEIEKEMQRAVMEFPEVRMVVSKIGRSEMGNDPQEPNAS
                     DPVVSLRPMDEWTTAKTKSALDNAIRKRIEKVPGANYLLSQPIQQRVDELLSGVRSEA
                     TIKILGDDLSVLRSTAEKIQTIMASVRGVGDVRVEQLFGQTYLTIDIDRSKIARHGIN
                     VAHIQEIITTAIGQEPATRVYEGNKRFDLTLRYPEKYRNSVDTIKNILLTTASGALIP
                     LGDLATVELQEGPSLISREGLQRRIYVGFNTLGRDIESVVAEAQAKIGEQIHLPAGYH
                     FVWGGSFENMQRAMARLKIILPITIGLIFILLFASFNSVRHAALIIMNLPFAMIGGIV
                     ALWLTGEYLSVPASVGFINLFGVAVLNGIVLVSYFNKLREDGLQGDEAIIKGCTLRLR
                     PVLMTALVALLGLMPLAFAHGIGSEVQRPLAVVVIGGLVSSTLLTLIVLPVLYRWLDR
                     PDKPAALEEEPAR"
     gene            23082..23396
                     /gene="sugE"
                     /locus_tag="PK224_14775"
     CDS             23082..23396
                     /gene="sugE"
                     /locus_tag="PK224_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HRB17057.1"
                     /translation="MAWTLLCVAGLFEICWAIGLKYTEGFTRPWPTVGTLVAMAASFG
                     CLAQALKTIPVGTGYAVWTGIGAAGTAVLGMVLFAETASTIKVLSLLCIVLGIIGLRS
                     TS"
     gene            23438..24625
                     /locus_tag="PK224_14780"
     CDS             23438..24625
                     /locus_tag="PK224_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HRB17058.1"
                     /translation="MLPLTTPTQHESPLLHFPNRNTPKPTGWTLGEWLLLLPVGTAAL
                     FFHYGHEWLSDLSSPVRLSAILGWLLLVIILSAFAVLRHAEHLAERLGEPMGTVILTL
                     SVTGIEVMMIAAFMYTGNGNASLARDAMFAVVMIVLNGMVGLSLLLGGLRYHEQTYNL
                     QGANAFLAVIVPLAVLGLVLPSYTISSPGPTFSPHQSTFLIVMSLGLYGVFLAIQNLR
                     HRDYFVAPRSQSKRKMALLHQTEQAPKTPVWRHTLLLVAYLLPLVILSEHVATPIDYT
                     LRLWKAPHALGGVLVAGLVLAPESLGAVRAALANQLQRSVNILLGSVLASISLTIPAV
                     LAIGFFTESTIILGIDTVDTILLVLTFVVSMLTFAVRRTNVLLGAVHLLLFLAYLMLI
                     FEK"
     gene            24817..26238
                     /locus_tag="PK224_14785"
     CDS             24817..26238
                     /locus_tag="PK224_14785"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB17059.1"
                     /translation="MSGTKHKVFTILLVEDDAGDVDMFLRTVEHELPRHEDEQVTLVI
                     NATAEGALERLQQQPIDLIIADVRLPGMSGIELLRRVQDLNRRIPVIIVSWVHAIETV
                     IDAMRHGAFDYIVKPYDTMDLSARIHRAMRMSEILLQASPDEPAAPRIPFKNLVGVSS
                     PIRNVMAMIEAIARVPSTTLIIGETGTGKELIAKAIHERSLDSDGPFQVVDCTTFSEG
                     TVESELFGHVRGAFTGAVADRTGLIESGSHGTVFLDEIGDLPANLQAKLLRVLEAGEV
                     RAVGSTQQRKVNVRFIAATNQDLAEKVKRNEFRKDLFFRLNVMVIRVPPLRERTEDIP
                     VLARHFIARYAKEFTKRVEDLHPSAVTELVAYPWPGNVRELRNVMERAVMLAKGDRIG
                     IADVAGMLAVPDERQREAPEEDYLHLPYAKAKEQVLEAFNQRYISTKLTIHQGNVTHA
                     AQDAGLPRSYFHEIMRRYTKDEK"
     gene            26725..30168
                     /locus_tag="PK224_14790"
     CDS             26725..30168
                     /locus_tag="PK224_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRB17060.1"
                     /translation="MQVSVSRRQFLKISAGTVAAVAVADKVLALTALQPVIEVGNPLG
                     EYPDRSWERVYHDQYRYDSSFTWCCSPNDTHGCRVRAFVRNGVVMRVEQNYDHQTYED
                     LYGNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKQWMDDGSPELTPDVKRK
                     YKFDSRFLDDLNRVSWDTAFTYVAKAAVLIATRYSGEAGARRLREQGYAPEMIEMMKG
                     AGVRTFKHRAGMPVLGIVGKMMNTRFNGGCLPLLDSWIRKVDAEKAQGGKYYSNYTWH
                     GDQDPSHPFWNGTQNCDVDLSDMRFSKLNTSWGKNFVENKMPEAHWKLESIERGARIV
                     VITPEYNPTAYRADYWIPLRPETDGATFLGACKIIFDENLQDIDYIKEFSDLPLLVRT
                     DTLQYLDPRDVIADYKFPDFSKSYSGRIQSLKPEQIERLGGMMVWDLAKGKAVPLHRE
                     QVGFHFKESGIDPALTGTFRVKLLNGREIDVMSIYQMYQVHLQDYDLDTTHQITRAPK
                     DLIVRWARDSGTIKPAAMHNGEGVCHYFHMTEMGRAAALVMTITGNIGKFGTGCHTWS
                     GNYKAGIWNAVPWSGAGLAVHTGEDPFNLTLDPNAHGKEIKTRSYYYGEEVGYWNHGD
                     TALIVNTPKYGRKVFTGKTHMPSPSKVRWVTNVNILNNAKHHYDMVKNVDPNIEMIVT
                     QDIEMTSDVNHADVAFACNSWMEFTYPEMTGTVSNPWIQIWKGGIRPLYDTRNDADTF
                     AGVAAKLAEMTGDARFRGVFHFVYMNRVDVYPQRMLDASATCYGYSADVMLKSEKGWM
                     VMGRTYPRHPLWEETNESKPQWTRSGRIETYRIEPEAIEYGENFISHREGPECTPYLP
                     NAIFSNNPFIRPDDYGIPITAQHHDDKHVRNIKLPWAEIKRHPNPLWEKGYQFYCVTP
                     KTRHRVHSQWSVNDWVQIYESNFGDPYRMDKRTPGVGEHQLHINPQAAKDRGINDGDY
                     VFVDGNPVDRPYRGWKPSDPFYKVSRLMIRAKYNPAYPYHVTMAKHAPYVATAKSVKG
                     HETRPDGRAIAVDTGYQSNFRYGAQQSFTRSWLMPMHQTDSLPGKHTIAWKFKWGYAI
                     DHHGINTVPKECLIRITKAEDGGIGARGPWEPVRTGFTPGQENEFMIKWLKGEHIKIK
                     V"
     gene            30277..>31553
                     /locus_tag="PK224_14795"
     CDS             30277..>31553
                     /locus_tag="PK224_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit beta"
                     /protein_id="HRB17061.1"
                     /translation="MPEVYNWQLGRKMLYPYEERHPKWQFAFVFNINRCLACQTCSMA
                     DKSTWLFSKGQEYMWWNNVETKPYGGYPQFYDVKITQLIEQVNPGGQVWNVRVGRKHH
                     APYGVFEGMTIFDAGAKVGQAAIGYIPTDQEWRFVNIYEDTATSMRALVENIDKSGFT
                     RDEPWRLSGSSLPEHETYFFYLQRICNHCTYPGCLAACPRKAIYKRPEDGIVLIDQNR
                     CRGYKKCVEQCPFKKPMYRGTTRVSEKCIACYPRIEGKDPLTGGEPMETRCMAACVGK
                     IRMQSLMRIGEDGLWAEDRWHPLYYTIRVEQVALPLYPQWGTEPNGYYIPPRHSPRGY
                     ARQMFGPGVDNAIEKYLVPSRELLAVLQLWRASQQIVFRYDVIPGPKVFETQIHGKRF
                     DMYNDTVLGFNKSGKEVARIQVEEPIYIRPAERV"
BASE COUNT         6771 a         9706 c         8896 g         6180 t
ORIGIN      
        1 tgcttgattt ccgaccacgg cagcttgata ttccgcaccg tcttatcgtc atggtgctgc
       61 gccgtgacgg ggattccata atcctcaggc cgaacgtagg gattactcgt catgatggca
      121 ttcggcatat acggggtaca ctccgggcct tcccggtgtg agatgaagtt ttccccgtat
      181 tcaatggcct ccggctcgac gcgataggtc tccagacgcc ccgaccgtgt ccagtgcggc
      241 ttggactcgt tggtctcttc ccagagcgga tggcgcggat aggtacgaca catcaccatc
      301 caaccctttt ccgacttcaa catcacatcg gcgctgtacc cgaagaacgt actcgacgca
      361 tcaagaatgc gctgcacgta aatatccacc cgattgtgat acacaaattt atacgtatcc
      421 gacatccgct gttcaccggt gatctctttc agcttcgcgg cgactcccgc gaaggagtcg
      481 agatcgttcc tggtgtcgta cagggggcgg atgccgccct tccagatctg cacccaggga
      541 ttcgacacgg tggccgtcat ttctggatag gtaaactcca tccaagaatt cacggcgaat
      601 gccacatcgg catggttgac gtccgaggtc atctcgatgt cctgcgtgat cagcatctcg
      661 atgttgggat cgacgttttt caccatgtcg tagtggtgct tggcgttatt cagaatgttg
      721 acgttcacca cccagcggac tttgctgggg ctcggcatgt gagtcttgcc ggtgaacacc
      781 ttgcggccgt acttcggcgt gttgacgatc agagcattgt ccccgtggtt ccaatacccc
      841 ggctcctcac cgtaatagta tttgcggtat ttgatttcct tgccgtgcac atcttctttg
      901 agattcaaat tccagggatc ttcgcccaaa tagacgctgg cgcctgcgcc ggaccaaggc
      961 gccgcctgcc aaatgccgac tttgtagtta ccggaccagg tatgacaacc ggtgccgaac
     1021 ttgccgatat tgcccgtcag catcatcacc aacgccgccg ctcgtcccat cgatgtcata
     1081 tggaaatagt ggcagacgcc ctctccattg tggatggccg cgggcttcac cgtgccgcaa
     1141 tctcgcgccc agcgcacaat gagatccttc ggcgagcgat tgacctgatg taccgtatcc
     1201 aggtcatagt cctgaaggtg aatcgtatac aattgataga tgggcatgac gtcgacttct
     1261 cgcccattga gcaacttaat ccggtaggtg cccgtcaagg ccgggtcgat gccgctctgg
     1321 gccaggtgaa ggccgacttg ctcacgatgg agcggcacgg ctttcccttt ggccaggtcc
     1381 cagaccatca tgccgccgag gcgctgcact tgatcctggg tcaatccctg cacgcgtccg
     1441 gagtacgact tggtaaaatc cggcacccga tagtctttca acacttcatg cggatccaga
     1501 tactggaggg tatccgtccg cacgagcaac ggcatgtccg taaagccctt gataaaatcg
     1561 atgtcctggt agttctcatc gagaatgatt ttggaggcgc cgagaaacaa cgcgccatcc
     1621 gtctcaggac gcacgggaat ccagtagtcc gcgcggctcg ccgtggggtt atactcaggg
     1681 gtgatgacga cgaggcgagc cccccgctcc atgctctcca atttccaatg cgcttccggc
     1741 atcttgtttt cgacgaagtt tttgccccaa ctggtattca gcttggtgaa gcgcatgtcg
     1801 gacaaatcga cgtcgcagtt ctgcgtgccg ttccaccagg gttgagacgg gtcttgatcg
     1861 ccgtgccagg tgtagttgtt ccagtagcgg ccgccctgcg cctgatcggg attgaccttc
     1921 cggatccagc tatccaacaa cggcaacacg cagttgttga acctggtatt ggcatgtttc
     1981 cccatcatgc cgaggatcgg cataccggcg cggtgcttga atgtccgcac gcccgcgccc
     2041 ttcatcattt cgatcatttc cggcgcatag ccctgttcgc ggagacggcg ggcaccggcc
     2101 tcaccgctgt aacgagtgcc gatgacgatc aaccccttgg cgacataggt gaacgccgta
     2161 tcccaggaca cgcgaaccat gtcgtcgagg aagcggctgt cgaacttata cttccgcttc
     2221 gcatccgacg tcagctcggg ggagccgtcg tccatccact gtttccagcc cttccgcatc
     2281 aacggcccct tcaagcgata ggggccatac acgcgacggt ggaaggtgaa gcccttcaag
     2341 cacatgcgcg ggttgtgcgc gaacgtgccg cggttgccgt acagatcttc ataggtttgg
     2401 tggtcatagt tctgctccac gcgcatcacg acaccgtttc ggacgaacgc ccgaatgcgg
     2461 caggcgtggg tgtcgttcgg tgagcagcac caggtgaagg atgagtcgta ccgatattga
     2521 tcgtggtaga cgcgctccca cgatcgatcc ggatactcac ccaagggatt gtcgacctcg
     2581 acgaccggtt gcagcgcagt caacgccaga gccttgtcgg ctacagctgc cactgcgacc
     2641 gtccccgccg agaccttcaa gaactgcctg cgggataact gcatcatgaa tacctcctca
     2701 taagaagaca agacacgcta ccgacgccac gattcatggc gtccgcacaa cacttccccc
     2761 ataaccggaa caggccaatg caaatgaacc ctccttccat gccatgcgga tggttttccc
     2821 gccgcacgag cggccggtcg tacggcggcg cggcgattct cagctctctg cccgaggaac
     2881 cgcgcgcatc agtgagaaga tgaaaagaat aaaaaatcac gacgtcccct tgtgcacctc
     2941 acatgccacg aggacgaatc cagacagaat gtctatgtcg ttgaaaaacc gaacggcaga
     3001 aatgaagatt tcatgaaggt cgcgggcggc acaaaacagc cacgaagaca cgtggtggca
     3061 taccacgatt ccaccacgat caatcgtggg ctggaacatt catgccgtga ttagagagag
     3121 acaggcacgg aggaactggt tacatgcggg aggggcggcg aatggccaat ttctgcatcc
     3181 tgctgcggag cgtgctcggg gctaacccca actggtccgc agctccaccg aggccttcga
     3241 tccgccagtc gagcagcgtg agcgtttgaa gaatatggtg tcgctcttcg tcatgcaacg
     3301 ttcgtcgagg acctgcttcg ggcgagacac ctacattcac catcgccgga tcgatacgca
     3361 acaggcgctc gtgacacagg atcaatgcac gctccacgat gttctcaagc tcccgcacat
     3421 ttcccggcca ggcatattgc accaaccgct ccatcgacgg cccatcgaag tcttcacaat
     3481 gtcgcctcag ccgctgggaa tgaacccgca ggaaatgtcg ggcgaggatg ggaatgtcct
     3541 ccgggcgttc tctgagcgga ggcatatgga tgggaaacac gttcaagcgg tagtacaaat
     3601 cgctgcgaaa acgcccctgt tgaatcgctc ggtgtaaatc ggcgttcgtg gcggcaatca
     3661 cccggacatc caccggaatg gcattcgcgc cgccgactcg atcgaccaga ccgtcctgga
     3721 gtacccgcaa gagcttcgcc tgcgcctcaa gcggcatctc gccgacctca tccagaaaca
     3781 acgtaccgcc atcggcaagc tcgaaccggc cgatccgttg atggtcggcg cccgtaaagg
     3841 cgccccgctc gtggccgaac aactcgcttt cgatcaatcc tgaggggagc gccgcgcaat
     3901 tcaatcgcac aaaggcccga tcacgccgca aactcctctc gtggatggcg cgcgccagga
     3961 cttccttgcc ggttccggtc tcccctgtca ccatcaccgt ggcgcccgtc ggagccgcct
     4021 gccgggcgag cgtcagcact tgctcgaacg cctggcttcg ccccacgacg atgccaaaat
     4081 cctgattggc cttcagctcc tccgccaagt acacgttttc ccgcgccaac tgttctttca
     4141 aacggttgat ttcttcgtag gcgttgacgt gagcgatggc gaagccgatc tgcgtggcca
     4201 cctgctggag aaaatccacg tcggccggat ccggatgccc cgcctcgacg ctgccgatgt
     4261 tcagagtacc gagacacgct tcctggacga ccatagggag gttgatcatg cgaccaagcc
     4321 cttcctggag ataactctca tcctcgataa acagtcgctc tcgctgaagg tgaggccgca
     4381 cgtgtacctg atgatgatcg tagacccacc ccaccgcgct gtgcgcgcga gggatcatcg
     4441 cgtcgcttct gagcaccacc ttcggcagat tcgtttccac cgcataaaag cgaaaggcgt
     4501 ccgacttcaa atcgtagacc gtgattccag cccgctccca ccggacgact ttcgccaact
     4561 gatccgtgac agcgcgaaac aggctctgcc ggttgcgctg agaattcagc acattggtga
     4621 cggacaacag ggtgcgatag ttgagcgccg cgttatgcac gacgtgttcc tttttcccgt
     4681 aacatagacc ctctatgaac ggctcactga ccctgtgcgc tggagcaaca cgtcgtcagg
     4741 agaggcgaag gcgcgcacgg cactgaactg caggtaggcc tcggtggcgg tggtgacttg
     4801 tgcgctgtgg gcatcatagg ctgggcccat tgctgattcc agtcgctcgt cgaatcttca
     4861 tcaaacttta atgtaacgcc ggcggcagtc tggcggatca cctggttcgg taaggcttgt
     4921 tttcccgatg ctccaacagg agaactaatg acgtcctgcg cccaaggcac gtctgccctc
     4981 atccgtttca aagcctggtc tgagccgcga gccactgcgc cctgcctcgg gccctaacga
     5041 cccggagcct ggaccgattg aaggcgcgag aggcgtcgtc gtgactccca gatcgggact
     5101 tcttcgcggc acgaacgcac tcggcaccgg atcacgatgc gtgcgcatcc ggtcgcggtc
     5161 caccggaatc cgtgcttcag gcgacatacg catatcgccg tttctggaag tcatccgctg
     5221 aatctcctcc tcctcgtcga gaagggaatc gatcgacgct ccgcgatcgc cgaccaccaa
     5281 attggtcgcg atcacttcct ttgccagaga ttccgccata ggcaattcgt ccgcgtagac
     5341 atcggtctcg tattcggccg tggagggggt cgccaacgag gtccacacgg attgcccgga
     5401 ctcggtgatg ttgatactgg tcacggtcga cagacccgga cggatcggat gttcccgtag
     5461 ttcgtctgcg ggtaaggcga tacgcaccgg gacgcgctcg acgatgtgaa tgaagttccc
     5521 ggtcgaattg tccggaggca acagggcgaa cgcgcttccg ctccccggca ccagcccctc
     5581 gaccgtgcca tggaacgtct gcttcgatcc gtagaggctg acgttcacca gggccggctg
     5641 gccgggacgc acatgttgca gttccgtttc acgcaaattc gcttcgaccc acagatgatc
     5701 cagcggaacg atggtcatga gcagcacccc gggctgaacc cgatctccca cctgggcttt
     5761 gcgcttggcg acatatccgg aaatgggagc ccgaatctgt tggcgcgcat actccaggtg
     5821 cgcttcgatc agctgatgct tggcgagatc cacagccgga tgggccatga cggtggtgcc
     5881 cccgatctgc gcatccagag aatccagctc ggcctgggtc tcccgcacct ccgcttccaa
     5941 gctcgcaatt ttgtccgcgg tattttgtag aatctgtttg gagatcgcgc cgctcggagc
     6001 ggcccgttga taccgatcca tatcgtgctc ggccaactcc agccgggccc ggcgcgagcg
     6061 cagtttttcc gcaagttgct tccgattgat aaacaacgac gcaatacgac ggacttcctc
     6121 gcccaaccgg ccacgggccc tccccagtgc agcgtaggct tgatgctcat ccaacttgat
     6181 catcaagtcc ccccgattga cgaactgcgt ttcctcggcg agcacctgcg tcacgatgcc
     6241 ggacgcctgc gccgccaccg gcaccaggtt ccctgtgacg taggcattat ctgttcgtac
     6301 ccaaaaccgg tcgtgcgtat accagtagac cagatatgcg acggtagcga ccaggaccag
     6361 accggccacg actaatagcc gccgattccg tcgcgcacga atggccttcg gatggatgcg
     6421 gtaggggccg gccggcggcg ggccaccaga agattgcgga tgttgagaca tatctgcggt
     6481 tgtggttgtc atggatttag ctcggatcct gtttcggtcg tttttcaatg tccggattgt
     6541 gatacccgcc gcccaaggat tcgatcagat cgactgccgc caccagttgg tcgctctcaa
     6601 gcgccctgag cgcatactcc tgctccagta ccggataacg atgccgcaac acttcccgat
     6661 catcatcgag cccattcacg agtcgcacct tcgccaaccg ccagtcgtcg cccaacgagg
     6721 ctaccagccg tttgtgcgaa gccataattt cgttcgtggt ctgccaggca ctcaaactgt
     6781 cggcgacttc ccgcatcgcc tccagcagcg tgtcgttgta gagttccacc gccgcatcgt
     6841 attccgcccg ttgcgcaccc aattcaccgc ggagccggcc gccttcaaac cagggcatgc
     6901 gcaatccggg cgccaagccg taggagaagc tttgaccgct gaaaagaaaa ttggccaact
     6961 tgtcggtccc cttggccaac gtcaacgcat tgaatcccac gaaccccgtt aaatcgatgg
     7021 tgggataaaa ctgggtcgtc gccaccctga ctaaacgcga agcggcatga gcgcgataga
     7081 gggcagccgc cagatcgggc cgatggacga gtaatcccag ggacagatga tccggtgccg
     7141 caatctgatt ggggacgatg actttcggct tggcaaagag atgggccccc tcatcaggcc
     7201 ccttgccgac taatcgcgcc agcagatgac gctgcacatc cagctgatcg cggatcgctg
     7261 cctggcgctt gaacgcggct tcatagtcgg ccatcgcgat tttgaccggc tggtcgttat
     7321 caagcccgag ccggaaccga gtctccgcca gtgtcttcag ctcgcggcgg atgccgacaa
     7381 tggtcttgac gatagtaagc tgctgttgaa gcgcctggcc gcggaaatag gcgcgggcaa
     7441 tgccggtggt cagtcgtagt cgaacttcgg ccgcttccgc ttcttcggcc gcagcatggc
     7501 ccaaagcggc ttccagcata gcccggttct ttccccaaaa atcgaactca taccggaagc
     7561 tcaacggatt gataatcccg tacatgatcc tgatgcccgc cacttccgga ttcagggctg
     7621 caaacacgcc atgttgtgaa atgcgctcgt aggtcaacga tgcatctgct tcgagaaacg
     7681 gcaacagccg tgcgccttcg accttcacga gtgaagtggc ctgccgcagc cgtgccgccg
     7741 catgttttaa cccggggtta tctgtgagcg actgctcgat caacccgttc agttccggat
     7801 tgccgaactg ttcccaccag cgatcctccg gccaattctg aagccgggac gtgacctccg
     7861 ccagcgtctc cgtcatttca ggcgcctcga gatattgcgc cggttgatcc cctttcggga
     7921 tccaggcaca accgccgaag aaaagcgcgc agccgatgcc gagggcaagc tgcaatcggc
     7981 gcctcattgt gacaggcaaa atcatggcac ctcctccatc atttcctcag cgcgcatctc
     8041 ccgcaactcc tcttcccgat tcagcgacaa aagtatgtgc gtgggatgcg cgaaccacac
     8101 caacacgccc aagccaatga acagataact tgcgaccaag aacgcgtcgc tcatgctcag
     8161 cagattcgct gcctgcttga ttatggcagc cagcctggct tgcacttccg caggggtcag
     8221 tcccgccgca ctcaacttgg ccgccaactg ccccatcggg tcgaacgaga tcgactgtcg
     8281 tccgccgaag tgatcggcca gatgcagttg atgaaaatgc gtccgacgga agagcacgat
     8341 gccttgaaag gtgatcccga atgcgccggc tgcgacacgc aacagattag cgatctcggc
     8401 agcgcgggtg accaaggggc cggataggcc gtgcagaagt aacaccgtca taggcgtgaa
     8461 gaacgacccc aggaagatcc cctccacgac catcggccag aacagctctt catatgaatg
     8521 gggatcgtcg tacagcccga tccaatagaa ggtcaaggca aagccgaggc tattaaaaca
     8581 aaccagccag cgcgcgtcga cgtacttgca aagatagtgc ataacggcga tgaccggcgc
     8641 ccccaggagc accatcggca acagcgccag gccggccaac ttcgacgaat accccaaaag
     8701 atactggatc tggacgatca gtagggacaa cagcccttgg atagaaaaaa agccgagcgt
     8761 gagactgatg acgccgatca gaaaatttcg gtgcaagaac agacggagat ccaggatcgg
     8821 atgccgctcc ccgagttccc agatgacgaa acagggacac gccacgagca gcaccaccgc
     8881 gatggcctgg aggaacggtg aatccagcca atcgaaatcg ttgcccatgt tcagcaacgt
     8941 ctgaagtcca aggagcaaca ccgccagcag cacaaacccg accaggtcga agcgcgcgta
     9001 ccggcgacga aagcctcgcc cgtacaacag ggcggccagc gtgccgacaa tcacgagtgt
     9061 cagcccgaaa tccaggtaga acagaaaacg ccacccgagc atgtacgcga tatacccccc
     9121 caccggcatc ccgatggtga agggcgtaat gctgaacagg ccccagacgg tcaaggctac
     9181 cgccttcaat cgcttggggt attcgttcag cagcatggac tgggcgatcg gcagcgtgat
     9241 gccaccggca atcccttgca caatgcgagc gggaacaaac tgcgcgagcg tctggctatc
     9301 accgcacaaa tacgacgcca cgctgtacac cataaaggcc ccgatcagca ggcggtaatc
     9361 gccgatacgt ccggacaggt atcgcgcgag aggaaacccc aatgccaatc cgaccataaa
     9421 gtcggtttgc gcccaggtaa gaaaactcgg cagcaccccg cccaaatccc cagacacatg
     9481 cggcaagagg gcgatgtacg acccggcatt gaacagcacc acgatatgcg acaagccgag
     9541 tacggcattg aaaagtacga atcgccatcc gtgtaaccgc ctgagataaa cagccgccat
     9601 cgccgatttc gtcgtcctta aaacgaggga gaataacccg gttgtttcgc ttgtgagtta
     9661 gggtgcccga ccggtgccgc cgccgggaca ggctgtgccg ccggggcaac gacacgatgg
     9721 gccgcataaa tcgctaatcc ggtaaacagc atgccgccga ccaaattccc cagcgtcacc
     9781 ggaatctgat tccacaacca ccaggtggag atgctgatat tgccacccag catcatgccg
     9841 accggaatca gaaacatgtt cacgaccgca tgctcgaatc cttgtgaaaa gaacaacagc
     9901 gtcggccccc agatggccag aattttcccg gaaaaggacg tcgacatgta ggcagcgacg
     9961 acggcgagac tgaccatcca gttacagagc atccctttgg taaaggccgc gagaaggccg
    10021 gaggagccgt gcgacacata atagttggtc ttcgcttcgg caaccgcaat gagcttggta
    10081 ccaaccgcat tgatatgggc atctccggcg gtggtcaggg caatcgcgaa caaccccgcg
    10141 tacaagacgc ttcccaggag attgcccagg aagacccagc ccatgtttgc cagcacatga
    10201 ggaaggccag cgttcttctg accgtcggcc acagccacgg ggagaagcgc gaaacttccg
    10261 gtgatgatct ccgcgccgag taggatggcc agggccaatc cgaacggaaa gaggaggctt
    10321 ccaatgatcc aatagcccgt ttcaacggca accgtcaccg ccatgctggt tccaattccc
    10381 agataggcgc cggccagcat gccgcgaagc accaactgac gcggcggcaa actcaacttc
    10441 agcgcgccgc cggctagcat actgtccacc acgccgtatg gcttcacata atccatcgcg
    10501 tcctccttct tcggttcgcc gaccatcacc cgccgcgcga tgcatggcac ggtcacggag
    10561 atcttcgtga cgcaacacat gagcaaggcg cgtgccgtag tatctttcca tcttccgtac
    10621 cgctctgaga cagatgggga agatcatttc gtgctgatca gaccggatca tccaccccca
    10681 tgggaaactt catgaatagg ggcgagatcg cacaggcgca cggtgcgaaa ccgctcattt
    10741 cgcatcccgc gataagaacc gcatgcggag cggggacccg gcacccgctg aaggcacagg
    10801 gatttctccc gacaggcgct ggtcccgaca gggcggatca ggcggcaaag ttgcacagag
    10861 acaagcgcac agactacgat gttgtcggag atgaagaccg cccttgagtg acctgctcag
    10921 caatctgccc attgcccgca agctctttct tgcgtcggtg atccctgcac tcaccgtgct
    10981 gcttctcagc gtcttgacct accgcacggt caccacgttc tccgacgacg aaggtcaact
    11041 caacgacatt tactactccc agcgattggc ctcggagtac cttcgactcg tcgtggacct
    11101 cgaaacaggg tttcgcggtt tcgtgctcac gagccaggag cattacctgt ttccctatcg
    11161 aaccgctcag gaccatgtgc tgaacatcgg caggacgctg gaagcgcagg tctccgccta
    11221 tgacgatcaa cgcgcgttga tcacctccgt ccaacaactc gtccggcagt tcatcgatga
    11281 aaaggaaacc ttgatcgatg ccgtgaaggc ggagcatccc gacgaggcgc ggctctacat
    11341 cgaggagggc aaagggcgaa cgttgatgtt gacgattcgc caggacatgg ggcgcttcga
    11401 acatctcgcc cagacggcac tgaataccaa actcgcgaaa ctcgcccagg atcgcgacac
    11461 catgctcatg accatcctcg gcggcggcct cttcgcgcta atatgcatgg tggcggcctt
    11521 gcacctcatc gcccactcca tcacaacgcc gctggagcgc ctggccaccg cagtgatggc
    11581 ggccgatacg cacccgatcc cgcacgtccc ggtcatgaca agaacggatg aaatcggcaa
    11641 tctatcccgc gtcatgcacg ccatgagctc ggccatccat tcccacattg cggcgctgca
    11701 attgtccgaa gccaacctgc gccagctcaa ccaggatctg gccggttcgg aggccaagta
    11761 tcgcagtatc gtcgaccatg cgcccttcgg gatcttcacg acccggggga tgaccctcgt
    11821 gttcagtaat cgctacaaca gcctcctcgc aggactcgat ccgagcgaag agaaggaccc
    11881 cgatgcggtc cgtcgcgcga tccacccgga ggaccgggag cgggtcatcg aggaattcgc
    11941 gcaggcggtg acggagggac agccctatga aacggtgttc cggtttctcc atccggacgg
    12001 caccgtcaga aaagtcttga gccgacgcat cccgattcgc gatcattccg gagaggtcgt
    12061 catgtaccag gggttcaacg tggacatcac ggccctcgat cttatgcaat cgcggctgcg
    12121 gcgggccgag cgactggcca cactcgggca ggtggccgca ggcatcgcgc acgaaatcag
    12181 aaaccccctt gtcggcatcg gctcgaccac gtccctcctg ctcgacgaca ccgatccagc
    12241 cgatgcgcgc cgccccgatc tggccgtcat tctgcaggag accaaacgcc tcgatcggat
    12301 cgtgaaccag atcatcgact atgcccgccc ccgtgagatc gtggcgttcg ccttcgacat
    12361 ggcacagctg gtgcaagagg ttataaaggt cttggatgag ccgctcacgc gcaaacaggc
    12421 gacgatccgt ctctcggcgc cggacgctcc ctacaccatt caggccgatc gcgatcagct
    12481 gaaacaggtg ctgctcaacg tcctgcagaa cgcgatcgaa gccagcccct ccgacgggac
    12541 gattaccgtc accctggttc aacaggaccg aggggtagaa ccgggactgg aagtcgctgt
    12601 cgcggatcgg ggcaccggga tcagccccac ccacctcccg cacgtattcg aaccgttttt
    12661 caccagcggc aaaccgcagg ggacaggatt aggactggcc atctgccgta acattctcga
    12721 ggcacacggc ggagaaattg ccctggacag tgaaatggga cgcggcacca ccgtgcgcgt
    12781 ctgggccccc ttacgacaac agccaccacg aatgcaggaa gaggaacagc atgcaggcca
    12841 cgatttacgt cacggatgac gaaccagcca ttcgatccgc catcgtcaaa cgcctttcgc
    12901 ggcgccacca caccgtgacc ggcttcgaat cgggcgagga tgtgatccgg gctgtcaccc
    12961 aacatgcgcc ggacctcatc ctgctggact tgaagatgcc cggcattggg ggcgtcgaaa
    13021 cactccggca gctccgcccg attgcgcccc agacgctgat catcatgctg acggcctatg
    13081 gcacagtgga agatgcggtc gaagcgatgc ggctgggcgc ctacgatttc ctgatcaagt
    13141 cggtcgatct ctccggtgtc gaccctgtcg tcgatcgcgc gatcgaattc ctcaccctgc
    13201 gccaccgggt cgaattttcg cttgaagacc aaaacagcgc gtacgcgctc tccaatatcg
    13261 atgcgcgcag tccggcgatg cagctcctgc tcggccaggt tcgggatgtg gcggagaacg
    13321 ccaaatccac cgtgctgctc caaggcgaaa cgggcaccgg caaggagttc ctcgcgcggg
    13381 tcatccattg caatagcccg cgcgcgtccg gccccttcgt cgcggtgaat tgcacggcca
    13441 ttccgaggga gctctttgaa agcgagctgt tcggacacga gcggggcgcc ttcacgggcg
    13501 cccatcagcg caaacgcggg ctactggaga aagccgaagg cggcacgttg tttctggacg
    13561 aaatcggcga cctagacctg gctatgcaag cgaagctgct ccgcgtgctg caggaacgca
    13621 ccttcaggcg cgtgggcggc acggaagacc tctccgtaga cttccgcttg atgaccgcca
    13681 ccaatcgcga cctcaagaag gacacggcgc gcggcagctt ccgggaagat ctcttctttc
    13741 gcttgaacgt ggtgaccttc gaactcccgc ccctgcgcgt acgcacggaa gatattctcc
    13801 cgctttccat gcaggccatg ctgcgattcg gcaaagagtt cggcaaggag gtcctcgaca
    13861 tcgaacccga cgcgcaagaa ctccttcagc gatacagcta tccgggcaat attcgcgaac
    13921 tccagaacat catcgaacgc gccatgattc tctgccacga caagaccctg accgccggct
    13981 gcctcccgcg cgaattgcgc gagcaggccc cgcacatcgc cgtggccatg gcgcaaggcg
    14041 agcacccctc ccttcgcatt gagatggtac tgggacaaca gacgctggcc gatatcgagt
    14101 cggccctgat cgaggaggtc gtgcgcctct cggatcacaa caaaaccctg gccgccaaac
    14161 atcttggatt gacgcggttc gccctggatc ggcgcctcaa gaagcaaatg gaacaggact
    14221 gagcatacag gcggcaggga gtgcattccg gggttgaacc gcgtcactcg ggaacggata
    14281 atggacacat gaatcaggca gccgatgacc ggcaagcata acagctggat tgccccaggc
    14341 atcgtcggat tgacctgcgg cctctttgcg ctcgatctgt tcatgccgct cggggtcgcc
    14401 atcggcgcgc tctatgccgg aatcgtgcta ctggcctcat tctccccgaa tccgcgactt
    14461 ccgctgctca ccgccgcctg cgccaccggg ctgctcatcg tgggggccgt ggcggggcct
    14521 cgcttgagca gcattccgct gtgggtggga gtcaccaatg cctccctcgg tctcgtcgtc
    14581 gtctggatct cggccgtgct gctgcaacag cggcatcatg cggagattcg actccgtcag
    14641 gcgaaagacg aacttgaagc gcgcgtcgcc gcccgcacca gagaactggc cgacgtgaac
    14701 cgcaccctgc tgcatgaaat ctctgagcac gttgagacgg aagaatctct acgcgccagc
    14761 gaacacgatc tcgccaccag ccggcaggaa ttgcgggacc tggcggcccg cctcctcacg
    14821 gtccaggagg aggaacgccg gcgcatctcc cgggatctcc atgacgacat caatcagcgg
    14881 ctggccatgc tggtcgtcca agccgagtcg ctggagagca gcctccctcc ctcggcgagc
    14941 gactgcagca aagaactgcg ctcgatccag gaccggctca ccgagctctc ggacgatgtg
    15001 cgccatctgg cctatcagtt tcatccgtcc attctggacg acctgggact gaccgtcgcc
    15061 ttgcagcgcc tggtcgatga gtgtgcgaca cgcgcgaacc ttcaaggcgc gttcgaagtg
    15121 tcgctggcgc cgccgaccgt gccgcaaacc gtttccacct gtctctaccg cattgcccag
    15181 gagagtctgg cgaatgtgat gaaacatgcg agggcgaata aggtgatcgt ttccctcgcc
    15241 tcgacggccg atgccatcac gctcaccgtg caggatgacg gagtcggttt tgacacgcag
    15301 caactggcga acagccatcg cggtctcggc ctgatcagca tggcagagcg cctccgtctg
    15361 gtccgcggca ccgtcaccat cgattcgatc cctcaccagg gcacacgcct ctacatcaaa
    15421 gtaccgcacg cggaggttcc cgtatgacca cgccccctcg agtgttgctg gccgacgacc
    15481 atacgctggt gctggaagga ttccggcgaa tcgtggaaca acgttgtgag gtggtggggg
    15541 cggtcgaaga cggccgcgcg ctcctcgacg cggccacgcg gctgcgcccc gacttgatcc
    15601 tgctggatat ctccatgccg ttattaaatg gagtcgatgc cgcgcgccaa ttaaagaagc
    15661 tgctgcccga cgtgaacctc atcttcgtca ccatgcatgc ggacccggcc tatgtgagcg
    15721 aggccttcaa agccggggca tcagcctacc tgcttaaacg atccgcggcc agagaactgg
    15781 agcaagccat cgactccgtc ttgaagggac agtatttcgt cacctccctg ctgaccaaag
    15841 atctggtcac gaccctctcc gcagggcagg ccggcctgtt tgcacaacgg caggaactga
    15901 cgccgcgcca gcgggaagtg ctgcagctca ttgccgaggg acgcaccatc aaggaaatcg
    15961 cctcgctgct gaatatctcg ccgaaaaccg tcgagttcca caaagcccag atcatctttc
    16021 atctcaacct ccgcaccacc gcagaactga cgaagtacgc gcttgcgcat gggctgacat
    16081 cggcctaacc aggactattt atgaatacct gctccgtcgg ccgatcctcc cgccaggcgg
    16141 ggccgttctc gttcgacctc ctcgacggac tgcacgtacg cctgcgtcgg tctccagcgc
    16201 ctcgcgacgg cattcatgaa taatccgggc tcgtcgctca actgaggtca gcctcgattc
    16261 cagccggctc ggattcatcg ctgaaatcgg cctcgggaca tttcacctcg gacctaggat
    16321 atcgcctagt tgtctctcct cttcacgttc gttaccctac gtgcatatgt ttcgccgttc
    16381 cccatgcctg tccgtcatgc tggctcggta cggtcgaagg aggtctggac gatggactcc
    16441 gcagccacac aatgccaggt ttgctggcga gtgcgggata acaggaccga aggaagctcc
    16501 tgtgcggtat ggtcagacat gcagacgttc atggacaacc acgatgtgac atcccgtagc
    16561 cccagctttt ccgaagggta ttgcccccag tgcaccaccc tgtatcatcg actcacgcaa
    16621 gaacatctct acgacgcata acagatccat acctgacacg accaggcgct gagccaacgt
    16681 gggtgagccg tagccgcgtt gtcgtccggt ctttacaaag aatccgacca ttgcatgctc
    16741 caggagagcg acaatggatc ggtcacagag gattgttcgc aaccgcatgc atccgaccat
    16801 ctactcgacg tactcgccag gccggctcca tcgaaccgca cagtgctgga ctcgcttcat
    16861 cgcaagtcat tgggagaccc ctcctacacc acacagactg tactctcact ggaatcaccg
    16921 gcctggcatc tatccctgcc ctctccgcac tgtagctcgc gacacaggct ctggcgcgct
    16981 tgatcgactt cgctccggga aggtgctacg ttgtttgaag caggacaaca ggcatcgcgc
    17041 agtggcgagc acggttctgc ggagggagga agtgcgacga tgaactacgc agatcaacgc
    17101 ggaccgatgc acgagccgca gcagcaatca gaggccgaag acggccgggt gtatggacca
    17161 cagctcggct cggcctccat tcctcaacga tccgcgccat ggggcgaacg gctggcgccg
    17221 gctctcagac tcgacgcaag aatcgcccct ttcgcgctcc tcgcgtgtct attcgtcgtg
    17281 ttctcgagtc cgatctccag cctcgccggc gaccgggcaa ccgcgccacc cgcggggccg
    17341 tctgatccgg tctattcgct ggaggcggtg ctgaattttg cattgacgcg caatcccaca
    17401 gtcgccagcg cggaaggcac catcgaccag aaccgtggac agcaggtggc cgcgtacacc
    17461 tatctcaacc cctccgtcag tgccaacagc ggcgtcggta aaatgcgcga tctgggactc
    17521 ttcgacccgt cagtgcggga gcgggtgacg gaattcaacc tgaccgtcgg acagccgatc
    17581 gagtggccgt caaagcgcgc ggcccgccag cgtgccagcg aagccggcgt agccgccgcc
    17641 tcggccggac tggcggaaac ccagctgaat ttagtcgccg atgtcaaggt cgccttttac
    17701 gatcttctcc tggctcagcg cgcgctgatc ctcgcacaac agaatctggc caccgtggag
    17761 gatgtcgata aggcggtacg gacgagggta cgcctggggg agagtccgca gttcgaagcg
    17821 atccgctccg gagtggaagt gttgaaagcc aatcagtcgc taaccagagc agccaatcga
    17881 gtgcgggtca accgggtgat gctcgacacc ctgacggccg gctcactcgg ccccacgtat
    17941 gctatcgatg ggcaacttca ccgtgtgggc ccgggattcg gcatcgacat tctgaccgaa
    18001 cgcgcgctca cccaacatcc gacgatcgtg cgcctgcgca agtcggtcga acaggccgat
    18061 catagcctgg aattcgagcg tcaggcccgc gtgcccaata tcacgatcgg aggcagctac
    18121 tggcgggaaa tcggtcggga agctttcacc ggtggcgtta ccttccccac ccccgtgtgg
    18181 gatcggcggc agggtgaaat catctcggct ttcgggagta aacggaaggg cgaagctgaa
    18241 tttcttcgtg cgcgaaatga attgatccgg aacgtcagcc agcacttcca ggatgcaaaa
    18301 acgactgcgg atatgatcga ggtctatgaa aaggggctct tgaaacaggc cgatgaagcg
    18361 ctacgaatcg cgaagttcag cttccagcaa ggggcctcca gcctgatcga agtactcgac
    18421 gcccaacggg tgcagcgtca aattttgtta gactatgcac aggcgcagtt cgatctttcc
    18481 atgtcactcg cgctcctgga acgtgccgtg ggaggcgcca tatgatgcac cacattaaac
    18541 acgctacgac ccgatcgaga tcattccgat ggacccataa ccggccgttt gtcctgatga
    18601 tgctggccgg catgtttgcg ttgctcgcct tgcccggatg cgatggccct cctcccaacg
    18661 cgacgggagc caaccccccg ccctctgccg acaagagcct ggtccgattg accaccgagg
    18721 aaattaaatc ggcgggcatc attgtccaac ccgttacacg ctcggagttt cgcacgatcc
    18781 gtgacttccc cggcaccgtc gaaccgaatg aacatgcctt agccgaaatc accacgctcg
    18841 tccgcgggcg ggtcatcgac gtctacgccg acctcggcag agaagtgaag ggagggaccc
    18901 tgctggctct gctctatagc agcgaactgg gtatggcgca atcggcttat ctgaaagcta
    18961 cggccaagct caacgtcgcc gagcgcgcct tccgccgggc ggaattgctc ttgaaagaaa
    19021 aggtcatcgg cgtggccgag ttgcaacgac gcgaaggaga aatgctcagc cttcgggccg
    19081 agctgcgaga ggcccgcgac cgcctcttga tcctgggtct cacggatgaa gacctgcgca
    19141 acttagatcg caatcacacg atccgctcac acgttccggt ggtggctccc ttcgacggcc
    19201 gcatcatcgc tcgtaactta accaaaggcg aggtcgtgga aaccacggaa aaactgttcg
    19261 tggttgccga cctcacggat gtctgggtga cggcagtcat tcccgagaaa gatatcccct
    19321 acatccggcc cgatcagaca ggaacaggtc aatcggtgga ggtccatgtg gccgcctatc
    19381 ccggacaagc cttccaaggg cgcatcacct atgtcggaga cgtgctcgac cccgcgaccc
    19441 ggacgatgcg tctccggctg gaattgccga atcccgagcg taagctcaaa cccgccatgt
    19501 atgccaccgt gcgcgtctat tccgaaccgg aggcgaacgc gcttctcatt cccgagtccg
    19561 ctgttcaacg cgaccgtgac cggcaattcg tctttgtcga acgcgagccc gccatattcg
    19621 aggcgcgcga tgtgaaatta ggcagttcga acggccgtga gattaaggtg ctggacggac
    19681 tgctggaggg agaatccatc gtgacgaacg gcgccttcgt gctaaaatcc gaactgctcg
    19741 gggaacagat gtaacccgat gctgtcctcc cttcttgcct tcgctctccg tcaacgtgtt
    19801 ctggtcgtcg tcatggcgtg catcctgacc gccgggggga tctatgcctt ccgcaccatc
    19861 gccatcgacg ccttcccgga cgtcaccact gtgctggtcc aggtcgtcac gaaagtgccc
    19921 ggcatgtctc cggccgaagt cgaacgtttc gtcaccttcc ccatggaact ccagctcatg
    19981 ggcgcgccgg ggctgacgga tattcgctcg ttttcaaagg tcggcctgtc gatgatcacc
    20041 gtcgtcttca aggacgatat cgatatttat ttggcccggc aggtcgtgct cgaacgaata
    20101 ctggaaatcc aggagctgct gccgcctggc tcctcatccc aattggttcc gaacaccacc
    20161 ggattggggg aagtctatca attctatctt gagggacggc acgacaacga caaagacttc
    20221 gtgatgagcg attcggacct catggagcgg cggacgattg aggactgggt cattcgaccg
    20281 ctgctcaaag ggctacccga cgtggtggat gtgaattcgc tgggagggtt cgtcaaacag
    20341 taccaggtga tggtcgagcc gggtctgctt cgtaaatacg gtctgtcgct tcacgatgtg
    20401 ttcgatgccg tggcgaagaa caacgcgaat gccggcggga acatcctgga gaaagacgac
    20461 gaaaaatatg tgattcgcgg tgtcgggctg atcaagacgc tcgaagacat cgaccacatc
    20521 gtcgtcaaac aggtgggtgg tacccccgtc tacgttcggg atgtggcgga ggtccggatc
    20581 gggcacgccg tccggcacgg cgccgccgtg ctgaatggaa aacatgaagt gatcaccggc
    20641 atcgtgctca tgctgcgtgg cggcaatgcc cgggacgtgg tgcaatccat caaggacaaa
    20701 atctcagaga ttcatcacaa acgcctgctg cctgacggat tgcgcatcat tccgttttac
    20761 gaccgaatcg aactgattac ggcagcgctg gataccgtct acaaagcgct gcttgagggc
    20821 attgtgctcg tggtcgtcat tctctttctg tttcttggca acgtgcgaag cgccctcatc
    20881 gtgaccgcca cactgatcgt gacgccgctg gtcaccttca tcatcatgga ccaggttgga
    20941 ctgacggcca atctcatgtc actcggcgga ctggtcatcg ctatcggcat gatggtcgat
    21001 ggctcggtcg tggtggtaga gaatgtgtat cggcatctcg ccgaacagcg agacgaccgc
    21061 ctcagtaaaa ccgaggtaat tttacatgcc gtcaccgaag tcggacaacc ggtgattttc
    21121 ggcatcctga tcattatcct tgtcttcctt ccgattctga cgctgcaggg catggaagga
    21181 aaaatgtttc agccgctcgc caatacgatc atcatcgcgt tactggtgtc cttggtcttg
    21241 tcgctgacac tctcccctgt tctctgcgcg atggcactga agcgaggaac ggaagaggac
    21301 acgttcctgc tccgctgggc caaatgggcc tatctcccga cattacgctg ggcgatgggg
    21361 catcgcgtca ccgtgctgac catcgcactc gggcttctgg gcgccagcct gtccctgttt
    21421 cccttcctcg gaggagagtt tattcccatc ttgaatgagg gggcgatttc gccccagacc
    21481 attcgttatc cgagcatcgc gctggatcga tctatcgaga ttgaaaaaga gatgcaacgc
    21541 gccgtgatgg agtttcccga agtgcgcatg gtggtgtcca agatcggccg ctcggagatg
    21601 ggaaacgatc ctcaggaacc caatgccagc gatccggtcg tgagtctccg tcctatggac
    21661 gaatggacca ccgccaagac caaatcggcc ttggacaatg ccatccgcaa acgcattgaa
    21721 aaagtgcccg gagccaatta tctgctgagc caaccgatcc aacaacgcgt ggatgagctg
    21781 ctgtccggcg tccgatcgga agccaccata aaaatactcg gagacgatct gtcggtgctg
    21841 agaagtacgg cggaaaagat tcaaaccatc atggcatcgg tcaggggcgt gggcgatgtg
    21901 cgggtcgaac aactcttcgg ccagacctac ctcaccatcg acattgatcg cagcaagatc
    21961 gctcgccacg gtatcaatgt cgcccatatt caggaaatta ttaccacggc gatcgggcaa
    22021 gaaccggcaa cccgcgtcta tgagggaaac aaacgattcg atctgacctt gcggtatcct
    22081 gaaaaatacc gaaacagcgt cgacacgatt aagaacatcc tgctgactac ggcctccggt
    22141 gccctgattc cgctcggaga tctcgccacg gtcgagctgc aagaaggacc ctcattgatc
    22201 agtcgcgagg gcctgcagcg ccggatctat gtcgggttca acaccctcgg ccgagacatc
    22261 gaaagcgtcg ttgcagaagc gcaagccaaa atcggggaac agatccatct accggccggc
    22321 tatcactttg tctggggagg ctcgtttgaa aacatgcagc gagccatggc acggttaaaa
    22381 atcatcttac cgatcacgat cggcttgatc ttcatcctct tgtttgcctc gtttaattcc
    22441 gtccgccatg cggcgctgat catcatgaat ctgccgttcg cgatgatcgg cgggatcgtg
    22501 gccctctggt tgacaggtga atatttgagt gtgccggcct ccgtggggtt catcaattta
    22561 ttcggtgtgg cagtgctcaa cggaattgta ctggtctcct acttcaacaa gctgcgtgag
    22621 gacggccttc agggcgacga ggcgatcatc aaaggttgca cactgcgact caggccggtg
    22681 ctcatgaccg cattagtggc actcctcggg ctcatgccac ttgcgtttgc acatggcatc
    22741 ggttcagaag tgcaacgccc gctcgccgtc gtcgtcatcg gcgggctcgt cagctcgacc
    22801 ctcctcacct tgatcgtcct gccggtgctg taccgatggc tcgaccgtcc ggataaaccg
    22861 gcagcgctcg aagaggaacc cgcccgctaa taacgttccc ctcggatggg gatgcgcact
    22921 ccgcattcca ttctctcgcc cccgcttcat gtccctagtg atatgccgag gttactcgga
    22981 agagatgatc gtgtaccatc tctagcctgg gagtgtttcc atgttgacgt tgatgctccc
    23041 tctctaagtc atacgtcacg aaggtcgatg taaccgaaac tatggcgtgg actttactgt
    23101 gtgtcgccgg actctttgaa atctgttggg ccatcgggtt gaagtacacc gaaggattta
    23161 cccggccgtg gcccacagtc ggaacattgg tcgccatggc agcgagcttc ggctgcctgg
    23221 ctcaagcctt gaaaacaatc cccgtgggaa ccgggtatgc ggtatggacc ggtatcggcg
    23281 cggcaggcac cgctgtcctc ggcatggtcc tctttgcaga aactgcctcg accatcaaag
    23341 ttctctcgtt gctctgcatc gtactcggca tcatcggact cagaagcact tcctgagcca
    23401 ggcggcagcg atgttcattg gcggattcac gtagaccatg ttgccactca ctacacccac
    23461 tcagcacgag tctccgttac tccacttccc gaatcgcaac accccgaagc cgaccggctg
    23521 gacgcttgga gagtggctcc tgctcctgcc tgtcggaact gccgccctgt tctttcacta
    23581 tggccatgag tggctgagcg acctctctag tcccgttcga ttgagcgcca tcctcggctg
    23641 gctcctgctc gtgattattc tctccgcctt tgccgtcctt cgtcatgcgg agcaccttgc
    23701 agagcggctc ggcgaaccga tgggaaccgt gattctgacc ttgtcggtca ccgggatcga
    23761 agtgatgatg atcgccgcct ttatgtatac gggcaacggg aatgcgtcgc tggcgcgtga
    23821 cgccatgttt gcggtggtca tgatcgtact gaacggcatg gtcggcctct cgctcctgct
    23881 cggcggcctg cggtatcacg aacagaccta caacctacaa ggggcgaacg cgttcctcgc
    23941 ggtcattgtc ccgctggcgg tgttggggct cgtgctgccg agttatacga tttcctcacc
    24001 cggtccgaca ttttcccccc atcaatccac ctttctcatc gtgatgtcct tggggttgta
    24061 tggtgtgttt ctcgcgattc agaatctgcg ccaccgcgac tacttcgtgg caccccggag
    24121 ccagagcaag cgaaaaatgg ccttgctcca tcagactgaa caggccccga agacacctgt
    24181 ctggcgccat acactcctcc tggtggccta cctgcttccg ctcgtcattc tctcggaaca
    24241 tgtggcgaca ccgatcgatt acacacttcg cctgtggaag gccccccatg cgttaggcgg
    24301 tgtgttggtc gccgggctgg tgctggcccc cgaatctctt ggcgcagtcc gggccgctct
    24361 agccaatcaa ttgcagcgct ccgtcaatat tctccttgga tcggtgctcg cgagtatcag
    24421 cctcaccatt ccagccgtcc tcgctatcgg cttctttact gaatccacca tcattctcgg
    24481 gatcgacaca gtcgatacca tcctcctggt cctgaccttc gtggtcagca tgctgacctt
    24541 tgccgtcaga cgcaccaatg tcctgctcgg agccgtccac ttgctcctgt tcctggccta
    24601 cctcatgctg atttttgaga aatagccgtc gggatccgac cctgcttgga gtgggccacg
    24661 gagttgattt gcctcgcctt cgcctcctaa gatacgggcc agaggacagc aattcacgca
    24721 acgcactcat ggccgacccc gggccagtct gaaccgcacc agaccaaaaa aaccattgct
    24781 gtgagcggac tgtaggagtt tccgagaaag acccgcatgt caggcaccaa gcacaaagtc
    24841 ttcacgatcc tgctggtcga ggacgacgcg ggagacgtgg acatgttctt gcgcaccgtc
    24901 gagcatgaac tgccccgcca tgaggatgag caggtcacgc tggtcatcaa cgccacggcc
    24961 gaaggcgcgc tggagcggct gcaacagcaa ccgatcgatc tgatcattgc cgacgtgcgg
    25021 ctgccgggca tgagcggaat cgaactcctg cggcgggtac aggacctgaa ccggcgtatc
    25081 cccgtcatca tcgtcagctg ggtccatgcg atcgagacgg tcattgacgc gatgcgccat
    25141 ggagcattcg actacatcgt gaagccctac gacacgatgg atctgtcggc gcgcattcat
    25201 cgggccatgc gcatgtcgga aatcctcctg caggcctcac cggacgagcc tgccgcgcca
    25261 cgcattccct tcaagaacct ggtgggtgtg agctccccga tcagaaacgt gatggccatg
    25321 atcgaagcca tcgcccgggt cccctccacc acactgatca tcggggaaac cggcacgggt
    25381 aaggaattga tcgccaaggc gattcacgag cgcagtctcg atagcgatgg cccgttccag
    25441 gttgtcgact gcaccacctt ctcggaaggc accgtggaga gtgagttgtt cggccatgtc
    25501 cgcggcgcat tcaccggcgc agtggcggac cgaaccggct tgatcgagtc gggcagccac
    25561 ggcaccgtct ttctcgacga aatcggcgac ctccccgcca atctgcaggc gaaactcctg
    25621 cgtgtgctgg aagccgggga agtgcgggcg gtcggcagca cccagcagcg gaaggttaat
    25681 gtccgcttta tcgccgcgac caatcaggac ctggctgaga aggtgaagcg gaacgagttc
    25741 cggaaagacc tgttctttcg cctcaatgtc atggtgatcc gcgtcccgcc tctccgtgaa
    25801 cggacggaag atatccccgt gctcgcccga cacttcattg cacgctatgc caaggagttt
    25861 acgaaacgcg tggaggatct ccatccctcc gccgtcacag agctcgtcgc ttacccatgg
    25921 ccggggaatg tccgggaatt acgaaacgtg atggaacggg cggtcatgct cgccaagggc
    25981 gaccgcatcg gtatcgcgga tgtagccggc atgctcgccg tgccagacga gcgacagcgc
    26041 gaggcccccg aagaagacta tcttcacctg ccgtatgcaa aagccaagga acaggtactg
    26101 gaagccttca accaacggta catctcgacc aaactgacga tccatcaggg gaacgtcacc
    26161 catgccgccc aggacgccgg actgccacgt tcctacttcc atgagatcat gcgccgctat
    26221 acaaaagacg aaaaatagcg gtgtcagcaa agcatgacac ccgcccacgc gaaaacaata
    26281 taagcaattg ttttcattga ataaaacaac aataaacctg cgcgtccgtg tttacagaca
    26341 cagataaatc tttatatttc aacatcttag tctgtacatc tcccatagta gtcagcagtt
    26401 actgacccct gagttttcgt catccattgc ccttcacttc cccatcccaa gacgaatcgc
    26461 aacatactga ttttattaat tttaattcct cattaccaag acgaaacgga gccacatttt
    26521 tggccatctt cggcacgtga attgtaatac tcctacaccg cgagcgaatg tccgctcgac
    26581 aatttcgaat tgcgaggggc gaatgtttcc aattcgacac acggaaagga ggtactccag
    26641 acaagattgc atccacgtga gtgtagtgag gttgtacgaa caggtgcatg agggactcat
    26701 gtgcctcaca aggaggtagc tattatgcag gtttccgttt cacggagaca gtttctcaaa
    26761 atctcggcgg gaaccgtcgc ggccgtggcc gtggcggata aagtcctcgc gttgaccgcg
    26821 ctgcagccgg tcatcgaggt tgggaacccg ctgggtgagt acccggatcg gtcctgggag
    26881 cgtgtgtatc acgaccagta ccggtacgac tcatccttta cctggtgctg ctcaccgaac
    26941 gacacccacg gctgtcgcgt ccgggccttc gtccggaacg gcgtcgtcat gcgcgttgag
    27001 cagaactatg accaccaaac ctatgaagac ctctacggca accgcggaac gttcgcgcac
    27061 aacccgcgca tgtgcttgaa aggcttcacc ttccaccgtc gcgtgtacgg cccctatcgt
    27121 ttgaagggtc cgttgatgcg gaagggttgg aagcagtgga tggacgacgg ctcccccgag
    27181 ctgacgcccg atgtgaagcg caagtacaag tttgacagcc gcttcctgga cgacctcaac
    27241 cgggtatcct gggatacggc cttcacctat gtcgccaaag ccgccgtgct catcgcgacg
    27301 cgctacagcg gtgaagccgg tgcccgtcgt ctccgcgagc agggctatgc tccggaaatg
    27361 atcgaaatga tgaagggtgc gggcgtgcgg acgttcaagc atcgcgccgg tatgccggtg
    27421 ctcggcatcg tcggtaagat gatgaacacc cgcttcaacg gcggatgcct gccgttgctg
    27481 gactcctgga ttcgcaaagt cgatgccgaa aaggcgcagg gcggaaagta ctattccaac
    27541 tacacctggc acggcgacca agatccttcc catccgttct ggaacgggac ccagaactgc
    27601 gacgtcgacc tctccgacat gcgcttctcc aagctgaaca ccagctgggg taagaacttc
    27661 gtcgagaaca agatgccgga agcgcactgg aagctcgagt ccatcgagcg cggcgcgcgg
    27721 atcgtcgtca ttacgccgga atacaaccca acggcctacc gcgcagacta ctggattcct
    27781 ctgcggccgg agaccgatgg cgcgacgttc ctcggtgcct gcaaaatcat tttcgacgaa
    27841 aatctgcagg acatcgatta catcaaggaa ttttccgacc tgccgttgct cgtacggacg
    27901 gatacgctcc agtatctcga tccgcgcgat gtgattgcgg attacaagtt cccggatttc
    27961 tcgaagagct attcggggcg catccaatcc ttgaagcctg agcagattga acggctcggc
    28021 ggcatgatgg tctgggacct ggcgaagggg aaggctgttc ctctgcaccg ggaacaggtc
    28081 ggattccact tcaaggagag cggcattgat ccagcattga ccgggacctt ccgggtcaag
    28141 ctgctcaacg gtcgcgaaat cgacgtgatg tcgatctacc agatgtatca agtccacttg
    28201 caggactacg atctggacac cacgcaccaa atcacccgtg cgccgaagga cctcatcgtc
    28261 cgttgggcgc gtgattcggg cacgatcaag ccggccgcga tgcacaacgg cgaaggcgtc
    28321 tgtcactatt tccacatgac ggaaatgggg cgggcggctg cgttggtgat gaccatcacc
    28381 ggcaacatcg gaaagttcgg tacgggctgc catacctggt ctggtaacta caaggccggt
    28441 atctggaacg ccgttccttg gtccggcgcg ggactcgcgg tgcacaccgg tgaagatccc
    28501 ttcaacctga ccctggatcc gaatgcgcac ggcaaagaga tcaagacccg ttcctactac
    28561 tatggtgaag aagtcggtta ctggaaccat ggtgatacgg ccttgatcgt caacacgccg
    28621 aagtacggac gcaaagtgtt caccggcaag acccacatgc cgagccccag caaagtccgc
    28681 tgggtgacca acgtgaacat tctcaacaac gccaagcacc actatgacat ggtgaaaaac
    28741 gtcgatccga acatcgagat gatcgtcacg caagacatcg agatgacctc ggacgtcaac
    28801 catgccgacg tggcgtttgc ttgtaactcc tggatggagt tcacctatcc ggaaatgact
    28861 ggcacggtct ccaatccgtg gattcagatc tggaagggtg gtatccgtcc gctgtacgac
    28921 acccgtaacg atgcggacac cttcgccggc gtcgcagcca agttggctga aatgaccgga
    28981 gatgcgcggt tccgcggcgt gttccacttc gtgtacatga accgcgtcga tgtctatccg
    29041 cagcggatgc tggatgccag cgccacctgc tacggataca gcgctgacgt catgttgaag
    29101 tcggaaaagg gctggatggt catgggtcgt acctatccgc gccacccgct ctgggaagag
    29161 accaacgagt ccaagcccca gtggacgcgg tcgggtcgta tcgagaccta ccgtattgag
    29221 ccggaagcca tcgaatacgg ggaaaacttc atttcacacc gggaaggccc ggagtgtacc
    29281 ccgtatctgc cgaatgcgat tttctcgaac aatccgttca ttcggccgga tgactacggt
    29341 attccgatca ccgcgcagca ccatgatgac aagcacgtgc gaaacatcaa gctgccgtgg
    29401 gcggaaatca agcggcatcc gaacccgttg tgggaaaagg gctaccagtt ctactgcgtc
    29461 acgcccaaga cccggcaccg ggtgcatagc caatggtcgg tgaacgactg ggtgcagatt
    29521 tatgagtcga acttcggcga tccgtaccgc atggacaaac ggacaccggg tgtcggcgaa
    29581 caccagttgc acatcaaccc gcaagcggcg aaagaccgcg gcatcaacga cggcgactac
    29641 gtctttgtcg acggtaaccc ggtggaccgg ccctatcgtg gctggaagcc gtcggatccg
    29701 ttctacaagg tgtcccgttt gatgattcgt gccaagtaca acccggcgta tccgtaccac
    29761 gtcacgatgg caaagcatgc cccgtatgtg gccacggcga agtcggtgaa aggtcacgag
    29821 acgcggccag acggacgcgc cattgcggtg gacaccggct atcagtccaa cttccggtac
    29881 ggcgctcaac agtcgtttac cagaagctgg ttgatgccga tgcaccagac cgactcactc
    29941 cccggcaaac acacgattgc ctggaagttc aagtggggct atgccatcga tcaccatggc
    30001 atcaacaccg ttccgaagga atgcttgatc cgcatcacca aggcggaaga cggcggtatc
    30061 ggagcgcgtg gtccgtggga accagtccgg accgggttca cgccgggtca ggaaaacgag
    30121 ttcatgatca agtggcttaa gggtgaacat atcaagatca aggtctagga cagaggtaca
    30181 tagtctgaga ggcacgcctc tcggcacttc gtcctcggtg cttgaaacga atgcgaacca
    30241 ttaactacat gatcatgttc agaaggagga ttcacaatgc cagaagtcta taactggcaa
    30301 ctgggacgga agatgctgta tccctacgag gaacggcatc cgaagtggca gtttgccttt
    30361 gtgttcaaca tcaatcgctg tttggcttgt cagacctgtt cgatggccga caagtcgacc
    30421 tggctcttct cgaaggggca ggagtacatg tggtggaaca acgtggaaac caagccgtac
    30481 ggcgggtatc cgcagttcta cgacgtgaag atcacccagc tcatcgagca ggtgaacccg
    30541 ggtggccagg tgtggaacgt ccgcgtggga cgcaagcacc atgcgccgta cggagtgttc
    30601 gagggaatga ccattttcga cgccggcgcc aaagtcgggc aggcggcgat cgggtacatc
    30661 cccacggacc aggaatggcg gttcgtgaac atctatgaag acacggcgac ctcgatgcgg
    30721 gccctggtcg agaacatcga caagtcgggc tttacgcgcg atgaaccgtg gcggctctcc
    30781 ggcagcagct tgccggagca tgagacgtat ttcttctatc tgcagcggat ttgcaaccac
    30841 tgcacctatc cgggctgcct ggcagcctgc ccgcgcaagg cgatctacaa gcggccggaa
    30901 gacggcatcg tgttgatcga ccagaaccgg tgccgcgggt acaagaagtg cgtggaacag
    30961 tgcccgttca agaagccgat gtatcggggc acgacgcgcg tctcggaaaa gtgtatcgcg
    31021 tgttatccgc gcatcgaagg caaagacccg ctgacgggcg gcgaaccgat ggaaacgcgt
    31081 tgtatggcgg cctgcgtggg caaaatccgc atgcagtcgt tgatgcgcat cggggaagac
    31141 ggcctgtggg cggaagaccg ctggcatccc ctgtactaca cgattcgtgt ggagcaggtg
    31201 gcgctcccgc tgtatccgca gtggggcacc gagcccaacg gctactacat cccgccacgg
    31261 catagcccac ggggctacgc gcggcagatg tttggcccgg gcgtggataa cgccatcgag
    31321 aagtacctcg tgcccagccg cgaactgttg gccgtgctcc agctctggag agccagccag
    31381 cagatcgtct tccggtacga tgtcattccg ggtccgaaag tgtttgaaac ccagatccac
    31441 gggaagcggt tcgacatgta caacgatacc gtgttgggct tcaacaagtc gggcaaggaa
    31501 gtggcgcgta ttcaggtcga agagccgatc tacattcggc cggccgagcg cgt
//
LOCUS       DAORRQ010000050        31360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_89_length_31360_cov_845.890148, whole genome shotgun sequence.
ACCESSION   DAORRQ010000050 DAORRQ010000000
VERSION     DAORRQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31360
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89_length_31360_cov_845.890148"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..3403)
                     /locus_tag="PK224_14800"
     CDS             complement(353..3403)
                     /locus_tag="PK224_14800"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17062.1"
                     /translation="MNLNDYETKYFSIYKAFAETVRFILKKAIQAAESLPRPQSIQYR
                     AKEIENLRRRLAEEGRLDTETLERDRRDLAGTRLIFYTNNDVNRFVQSSLIHDNFEIE
                     PDSTKVHHPTPENPRAQYRGIHYTVRLSEDRLRLPEYAKFAGLRCEIQVQTILEHAWS
                     ETSHDILYKNKMGKDFGARALEGIKHRFDRIADKYLVPAGYELQKAQQEYERVLQGKE
                     LFDKNVANILDKAQNNDERYDILSGLKDYAIPIYDDLPAAFEGLKGPLLRAVKGARTT
                     EPIPIEITDGKIDVKTEAVTNLVVEIVENLRYADVTGTLQLLIDIYRDEPNDAIRQQI
                     VNAVKKLSEYKIDVYNKVGPMLQMALVDYIANKNDAEVDSVWPIALTVWTEAIQSDIT
                     GEKWKADSVVLSTGALPASDQLKEVRDKALKALFAAYDRSTLDVQKRAVLSALGAATR
                     TPHQGQYSNQLLAITLRDATRIVEFLTERVKNTSYEVLQHLEHRFLYDYRRANGLTED
                     TDNRFGCQTDAKVLMAAILKFRDIINTDDRFVRYKVLVGFESVYPDHWTDEEFELEGA
                     DQYRRREAIRYIDEINPENENDWFNLIERCAETKSIDLATFPVFGNFISKLAESKPEV
                     ADRLLARASDDLRTFLPGFLNGLAVGSRADIYERILESELESARNLTSIAWHLRKSDV
                     KKPDFANRLLKRAIENGDQGAVTDCLLLALEYYGTEKVPDADTFVRDALTFLNDCKDP
                     RWVSQAWFLQKPTKFYEELTPERTAQLLRNLGYLRKINYQAERILVRLAEHQPAAVWD
                     YFGARLAREVADGDHVNDEEHFEAVPFQFHGLENALSKNPHLAISKGLSWFAQDRTLF
                     QFRGGRLLSNTFPNCTQEFSTALSDLVKAGSDTEAEFALAILQNYDGAISSHVVLKEI
                     VARSPDDQDKLDEVRVSIDNVGVTSGEFGLAEAWQARKESLREWLTDERPVVKAFAEK
                     HIAELDHMIASEQRRAESRREMRKRDYEEDGNESDDSIGNKGDQ"
     gene            4703..5425
                     /locus_tag="PK224_14805"
     CDS             4703..5425
                     /locus_tag="PK224_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17063.1"
                     /translation="MVRNILVIEDDRDIARLVELHLHDLGYTVKITHDGVEGLREALL
                     TSYDLMILDVMLPGMEGLELCRRIRAKSTYTPILMLTAKSSELDRVLGLEVGADDYLT
                     KPFSIRELLARVKALFRRAEAFASESLPKMANTIIAGDLQVNVEKRTVTLRGNAKDLT
                     AKEFDLLVHFAVHPGRVYTRAQLLDSVWGYGHDGYEHTVNSHINRLRAKIEQDPARPD
                     YILTVWGIGYKFRDVEDVERRG"
     gene            5429..6904
                     /locus_tag="PK224_14810"
     CDS             5429..6904
                     /locus_tag="PK224_14810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB17064.1"
                     /translation="MLRTLYGKFAAVLLILFCSIGVLYIFLTLFTTRMYLQEVNQKLN
                     RAIAKNLVADRLLSIQGEVNPYALKELFHVLMVVNPNIEIYLLDSKGTILTFSAPPEK
                     VKRSKISLEPVHRFLRETDAFPIVGDDPRDVTRQKVFSVSPFPLHGDPEGYLYIVLGG
                     EEYDSAVTMLQGSYILRLGLWAALTGLLFALLAGLLLFNWLTRRVGQLASTVESFAKS
                     DFSEQMQATLSIALPSRNRDEIDQLGGTFNLMAQRILQQMTTLKHTDTLRRELVANVS
                     HDLRTPLASLHGYLETLLIKEGTLSAEEQRSFLDIALRQSERLRRLVGELFELATLDS
                     HETQVHCEMFSLAELVQDVAQKFQLIAKQKQVELHTQFHADLPFVWGDIGLIERVLEN
                     LIENALRYTPSGGTVTLALSQQEHQVLVRITDTGCGIPEQDLPYIFDRFYRVGRHQRS
                     EGAGLGLAITKRILELHGSWVEVDSSVKVGTTIAFCLSTSS"
     gene            7151..7933
                     /locus_tag="PK224_14815"
     CDS             7151..7933
                     /locus_tag="PK224_14815"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRB17065.1"
                     /translation="MRNRLFFAILGFMVIFAQVAGAEVDREKLLKDPGVYATFAVFKV
                     GDQWGQLDKQAKAIAVSEVKKVFQQHSDKITVDAHLLRGLSEKADFFLRVHSKEMLHN
                     QNFLLDFMGTTFGKALKNTDTFNGITKALNYVPNFPEDMKAELKTPPPQGSPYVVVVP
                     IRKDADWWLMSQDERTAMMKEHTDPTVGYLKTVKRKLYHSSGLDDLDFITYFETAKLD
                     DFNNLVIGLLKVKENRHNKRFGDPLLLGTIRPLEEILEILAR"
     gene            complement(8174..8434)
                     /locus_tag="PK224_14820"
     CDS             complement(8174..8434)
                     /locus_tag="PK224_14820"
                     /inference="COORDINATES: protein motif:HMM:NF019069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HRB17066.1"
                     /translation="MRTTKAILSASALLLLVQTSMTPQAQAQVLTGDTRLACEAMLCL
                     AAGSNAPSECSAALAKYAALQAKTALQTAVNQKNFLKLCPKQ"
     gene            complement(8519..9184)
                     /locus_tag="PK224_14825"
     CDS             complement(8519..9184)
                     /locus_tag="PK224_14825"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB17067.1"
                     /translation="MSEPYRDLQTGVLVNKLGIMSATDLARAEYNITNLRIAELTVTP
                     VAGTFDLDHLKAIHKHLFSDVYEWAGQTRTMNVVKPDPEDPRWVSTFAPAKTIHDVST
                     SIREGLAEWKNLKELNQKDFTTLLATTYVTLNDMHPFPEGNGRSTQTLLVQLADEANY
                     TVDYAKVRPDEWNRAASRSTPQRQADDPTVTRKGDPTLIQDVFRRIVEPLKERVSPHD
                     RDR"
     gene            complement(9181..9369)
                     /locus_tag="PK224_14830"
     CDS             complement(9181..9369)
                     /locus_tag="PK224_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HRB17068.1"
                     /translation="MNVEDTRREAFRQADAILALEGMEKPPEMDGLQEAVIDGRMSFG
                     VAVSVTILEEQTRSRRRQ"
     gene            9706..10089
                     /locus_tag="PK224_14835"
     CDS             9706..10089
                     /locus_tag="PK224_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17069.1"
                     /translation="MKTPANPFTIHVIDAHDVVPYIAAPRPSIVNRLRPYITNALTRT
                     GRGLVQTSRVAVFVVTELSRMIVGAGIGAVTGLRLLYSFLAAMLEFFFIKLPVFVIGV
                     VFLLVTIAFSLGILDAMYRAVLAHH"
     gene            complement(10203..10592)
                     /locus_tag="PK224_14840"
     CDS             complement(10203..10592)
                     /locus_tag="PK224_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17070.1"
                     /translation="MKQYRIGRDSDTELIQVEVEERGTGYRLQHIPFHSKTGFELDSS
                     RPGSADLAIAIVADWLQEHPTTESLKTQFWECLALSGDFHARFLAPVDGETCLILGNE
                     IQVFMAGRSRYIIEREPSIDLITCDGC"
     gene            complement(10589..11296)
                     /locus_tag="PK224_14845"
     CDS             complement(10589..11296)
                     /locus_tag="PK224_14845"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HRB17071.1"
                     /translation="MMQSLTLPRVELSDGAVEAMKWLGLVLMTADHAQKYGMIPAVSG
                     IYEAGRLALPLFGIVLAYNLARVRSQNLAVYVRVLTRLLFCCAIASVPFIALGGLGFG
                     WWPLNCMGTLAVAVSIMYLIESRRPFWKTKAAVVFLLGGAFVEFWWPGVTLCVGAWLY
                     WKQPTWGALLLWLAGTASLTLINGNWWACASFAVLAGMASFVKIEIPRASRFFYIYYP
                     AHLTVLLLLGFRIQSHS"
     gene            complement(11300..12397)
                     /locus_tag="PK224_14850"
     CDS             complement(11300..12397)
                     /locus_tag="PK224_14850"
                     /inference="COORDINATES: protein motif:HMM:NF037421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17072.1"
                     /translation="MNELLEREGIEKDSTKETVLPREGQRGTLKPEPLLNQSAEAAES
                     LRRRFLEADNKFYFRGGSGAPDQVAFSDHGKRLTTDHDDPTVIHGMVLRAQEKGWTSL
                     RVKGSDEFKAEAWIQATIAGIEVEGYKPREIDRARLEERKGETPTRQPENRFEQVGPR
                     DRSTNRSEKEPDSLTAAQNVAVATLHTILRERGDSPAMIAAAMEEAKARLHGERLVVG
                     KVVEHGIDHYDHDPKSEKSYFVRVATAKGEKEIWGVDLARAIERSDAKPGDAVALIQR
                     AHERVTVQVPIRDEAGEHVGTSFQPAKRNMWEVVNLHTLGIGEREKVAAAARVATHEP
                     VVQVFDRGAARTDLAPEAARTREQERTRDGR"
     gene            complement(12411..13331)
                     /locus_tag="PK224_14855"
     CDS             complement(12411..13331)
                     /locus_tag="PK224_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB17073.1"
                     /translation="MNSDEVISQYRERVKGDQRDRVERNGLARQVDALTKQIQELKAY
                     VTLLQLQQQESNKGRLRQVRNVGAGSPPLLSEVPDKATANQKETPLALAVPPIAAVRS
                     DVPVPPMMPTEEGPTDRADSGQGSRIRTGGSVPRQTTRVVELASLQSVDRAGTPPAAI
                     RRAPEEEFREPMSVKLSEREELELHNSTVLFRVSEGKGLSDFVPTESMKAYLTKAIML
                     GQCIQVRGYTDGEEDVWFERKTARDRAEKVRRYLIDQGFAPMNIDVKVFPIGEHIADN
                     ATAEGRAKNRRVEIEFMDIEPASVWPQWKS"
     gene            complement(13454..15580)
                     /locus_tag="PK224_14860"
     CDS             complement(13454..15580)
                     /locus_tag="PK224_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRB17074.1"
                     /translation="MRLKIVAGVLAALLYIPLGLLGADYIAGAMFYVTNKLVPEEVSL
                     DTWPDSWRAYSEDPTQRKRLQLSAGMGLFALFGLPLVLVLSVMTRARSLHGDARFASP
                     TEIAGAGLYGDKGVIVGKYGRRYLIYGGQEFVLLAAPTRSGKGVSIVLPNLLNFNESV
                     VVLDIKMENFAYTSKFRQMHGQEVFLFNPFGTDNRSHRWNPLDAVHRDPNLRVGEIQA
                     IGHVLYPAENVKDAFWNESARNLFLGLCLYLLESPTLPCSLGELLRQASGKGQPIKEY
                     LQGLMAKRGTGDQRLSDDCTGALQRFCTTSENTMAGILATFTAPLTMFSNPIVDAATS
                     ATEIDLSAVRKRRMSIYVGIPANRLSDAALLVNLFFSQLIHHNTIDLPAMNPALKYQC
                     VLFLDEFPSIGRVDILAKSVGFIAGYNIRLFPIIQSESQLQSIYGEKDARTFITNHSC
                     QVMFAPREQQDANEYSHMLGTYTAKAVSTGMSHPRTWGNGRQASTSSNISDQARPLML
                     PQELKELGPTRQIINLVNTKPILCNKAFFYLDPVFVNRLKSASPSLAALGRKLPTQTE
                     LETAALVRKDLSVHIPILDVDLHKAKVERRIRPLTPNEPIDLARLTIDSGLLPPVVQR
                     DPPNVEDVTQLVDAFFSQLEWTEPDVPAGSGNVPAANGRQLLNRAAQEASERLAETVD
                     PTVESRVAELASPRIAFDLSVLERDS"
     gene            complement(15588..16697)
                     /gene="virB11"
                     /locus_tag="PK224_14865"
     CDS             complement(15588..16697)
                     /gene="virB11"
                     /locus_tag="PK224_14865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HRB17075.1"
                     /translation="MHSTPNNLLAHDTMVRELLRPLLRFLSQPGATEIVINRPGEVYV
                     EVGATWERFDAPDLTCSQLMALATAIATYTEQEIGPQKPILSAMLPDGERVQIVLPPA
                     VELGTVSFSIRRPSAWIKSLDEYEKEEAFNRYVWARSSELDARMGDLDKVERSLVDCL
                     DSKRLGDFIQQAVRAKKNIAVVGDTGSGKTTLMKSICQSIPADERLITIEDVRELFLP
                     NHGNRVHLLYSKGGQGVAKVTPSDLIASNMRMKPDRVLLAELRGGEAFDFLKLLTTGH
                     SGSITSYHAESCALAAERYVFMCKEHEQAAIYDAATLKRLVSLTIDVILHVVAHNHYN
                     AEGQPTHKERYVAEVHYDPIAKLVARFGAATLVRA"
     gene            complement(16678..18102)
                     /gene="virB10"
                     /locus_tag="PK224_14870"
     CDS             complement(16678..18102)
                     /gene="virB10"
                     /locus_tag="PK224_14870"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HRB17076.1"
                     /translation="MMEEKTMTEGKEELGIVSVNNRSGAAGHTAGKIVFVVILGSVVL
                     IGLLMGMNKWKANRKAEASKEEQAAKIENKPAQVGPKRVFDSDPVYQPNKGSSTASKG
                     GSQAASLTPGMIVPGVENGPNEIGRPIPLINGSGKGGGTQPGTQQPSRFGGDAILASS
                     NVPSGPRTQHAQATGAEAAISLVRDLLGNPRGSESMDQAGSTIGGAQAIPVGMSIPAG
                     DVSTTASDSIPAGPVGGSSLPTMGGMRGVGVPLAPGPAPSGAGSIGGLLTPSSTPKVQ
                     AGMLGDRNMILPKGKSIDCALTVRVVNEVAGMATCVLNSDVYSDNGRVVLLDRGSEAV
                     GEYSATMAQGQRRLFLLWTRIKTPNGVVINLNSPAADELGTSGLAGYVDNHWWDRLGA
                     AFLLSIVQDAIGYATAQAAGGGGQGQLSIYQNSATTGNRMAELVLQSTINIKPTLYKN
                     QGDRATIFVARDLDFGTVYALHAE"
     gene            complement(18110..19012)
                     /locus_tag="PK224_14875"
     CDS             complement(18110..19012)
                     /locus_tag="PK224_14875"
                     /inference="COORDINATES: protein motif:HMM:NF015487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HRB17077.1"
                     /translation="MRVVWCLIVVLVLPNVWASAAELPEPGELDQRVRYVTYKQDDVT
                     TVHVRRGVITRIVLGEDEKIVIAATGFLGDCAKSEAEWCIRADVGTNQVWVKPKDNAT
                     HNNLEIRTDKRDYSLEFNVLADNKLGRWRKPSDGHGLEDEAMYRVIFRHPIQLPNPQT
                     LMALNASLDQTKQARDDQQAVADRLAAFTPEPRNWAYSMEVLTGADDIVPALVFDDGR
                     FTYFSFPSNREIPAIFYISPAEEETRLNFHMEKEFAVVQRMGRRFVLRLGQAVVGVWN
                     DAYDMNGVPPANGVTVSGVSRVVR"
     gene            complement(19038..19781)
                     /locus_tag="PK224_14880"
     CDS             complement(19038..19781)
                     /locus_tag="PK224_14880"
                     /inference="COORDINATES: protein motif:HMM:NF016238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HRB17078.1"
                     /translation="MAEPTTVKASDEQTFYDQGRDWETDKGIRLEQSERRAWWVAGIA
                     CVLAFGLGIGLACLAPLKSTIPYVFSVDRATGNVELVSAADDRTVMGYQELLDKHWTQ
                     KYVIARESYYFRLLQADYDTVLSMSADEVGRDYARIYDGPNARDKKLGSGMELKVKVL
                     SVTLNADAVGLKATVRFEKTPKRTEAEASDLPQYFIATLAYEYRMSMKGQEKDLIQNP
                     LGYKVTSYRVDAEIGTASPPVPVAMMERS"
     gene            complement(19802..20524)
                     /locus_tag="PK224_14885"
     CDS             complement(19802..20524)
                     /locus_tag="PK224_14885"
                     /inference="COORDINATES: protein motif:HMM:NF019608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HRB17079.1"
                     /translation="MGAMKRVLVTLALVAMVFVGSVPKAEAGIPVIDAANLVQSIMQA
                     LSWIQQLQQMQQQITQAQSQIDAITGSRNMASLFNNLALAGVVPSDVNAVYGAIRSGG
                     ISGLTTAAQIIRNNRMIYNCEGKIGDALRICQNILNQNPQSQAYYANTFQMLEGRMNQ
                     IRSLTNSIDTTADEKASLDLQSRIAGEQAQVQNDTNRIITMKATAEAEEKAAEQEQRE
                     RVLKMIVPGMPGAFDTMAPWTP"
     gene            complement(20540..21577)
                     /locus_tag="PK224_14890"
     CDS             complement(20540..21577)
                     /locus_tag="PK224_14890"
                     /inference="COORDINATES: protein motif:HMM:NF016491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HRB17080.1"
                     /translation="MGVASYFENSVNNALTTYVVDFSDAVITQLVPIAVTAVTGYVMW
                     TGFSVMRGEAKDSVSTLIWRWFRVALIAGLALSGPEYRNIVKDGLDGIQGAFGVAFGA
                     GPTVGATIDNMIDPFYSLSAMIMTEASTGVLPQFSLFIAGFISAMAGLLMAFVALGIL
                     LVAKVSLALLFAVGPAFIFCAMFPITQRYAESWLSSALGAVFTNVLIMAVVAMLSSIL
                     RTACANIITSYGTTSVLTDVIGLFVLSASAAYVLINIQSLGAGLAGALSLGNPGGDAA
                     AFATGGLRGLVRALRREPDRSSTGELIGTGKGTAVNGTTPARLGLPAPTSSTGADLYQ
                     RGVIERLQRTT"
     gene            complement(21642..21884)
                     /locus_tag="PK224_14895"
     CDS             complement(21642..21884)
                     /locus_tag="PK224_14895"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HRB17081.1"
                     /translation="MGQISRWVLVLVVAIAAGCTNEATEPVQTVDWYLAHLPEMKAMR
                     EKCGNNPGELGKTPNCLNAEQAFIKYRLAPRTGTLQ"
     gene            complement(21905..24346)
                     /locus_tag="PK224_14900"
     CDS             complement(21905..24346)
                     /locus_tag="PK224_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HRB17082.1"
                     /translation="MRTRAEVTKAASAQIPASDYIPLGTPITPHVIKLSGTGDYLAIW
                     RLEGITFETADRADILVRKEGLHNFIRALGGGAYAVWSHKIRRVVKERLSGTSPNAFC
                     EELNDRYYQSFDNHRQMATELYLSVVYRPSTSKVMSFFKRMGKRSLQQIRDRETEHLT
                     ILEDTAKQLEASLKRYEPQRLGTYTKTDIVYSEMLAFLGYLINGVWEEIPFRRAPLSS
                     YLPTSRLHFGDKTGMVEIWHPNERKYAGFLDMQEYPQFSEPGMNNGILYGDDEYIETQ
                     SYSIYNKRDALKALDKQKGHLIASEDAATREIEQMDQAAEDLQSGLIDLGEYHYTVAI
                     FGQTMNAVAKSMAETRAMFQDGSGFKMSPVDVIPECAWYSQIPGNWNLRPREATITSR
                     NFVCLSPFHNFARGKQSGNPWGEALALFKTPSGQPYYFNFHASPEDKDSRDEKYPGNT
                     FICGTTGVGKTALEMSLLSFATKYQGLRCVFFDKDRGAEIGIRAMGGKYFPLKRGVPT
                     GFNPFQFAPTAHNLQFCEKLVAQLVKNQGDETPILSAKEQQDISHAVRTVMSEAATRE
                     VRCLSLVLQNLPATGDNSLKARLKKWTRGQPLGWAFDNPTDTQDFSGARLYGYDYTEF
                     LDDPEIRTPIMAYLLHITESLITGDPFIYFMEEFWKPLQDPMFSEFALNKQKTIRKQS
                     GLGVFVTQSPSDVLGHPIGKTMVEQSVTQIFLPNPRADHDDYVHGFKVTESEYRIIKN
                     LGEVSRMFLVKQGHGSAIVRFDLGSMPDILNIISGTTENVALLDSIRDEVGDAPEAWM
                     PLLHQRIATRRGLIK"
     gene            complement(24414..24746)
                     /locus_tag="PK224_14905"
     CDS             complement(24414..24746)
                     /locus_tag="PK224_14905"
                     /inference="COORDINATES: protein motif:HMM:NF016957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HRB17083.1"
                     /translation="MSDGFRDPIFKGCTRPAMLGGVPTLPLILICGLTLLLSVWSFYF
                     VSGYVSLFMVLMAIPVVVTMREITKKDDQRLRQVMMRARMRLRHLASRATWGAISYGP
                     LKFKMRRV"
     gene            complement(24751..25086)
                     /locus_tag="PK224_14910"
     CDS             complement(24751..25086)
                     /locus_tag="PK224_14910"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HRB17084.1"
                     /translation="MNTSIVDGVEMDGNKASGGSAAMIKAASFVLGFAVMLEANPAMA
                     QITKVNTVMTNVQTVLVGVAVTLFTLAIMWAGFKMAFQQAQWSEVSNVVIGGILVGGA
                     AGIAAWLIN"
     gene            complement(25169..25696)
                     /locus_tag="PK224_14915"
     CDS             complement(25169..25696)
                     /locus_tag="PK224_14915"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRB17085.1"
                     /translation="MLDVYSIVQTCASEIHPQTMLRVIAVESTGNPHAIGVVDGKLEW
                     QPTNRAEALATARMLERHGFNYSVGLGQVNKKNFAKYGVTLEQAFDPCTNLRVASAIL
                     KDCYLRAKGGKATKQDIQRALRDAFSCYYSGNFSAGYRLGYVKKVVSAVPVISSTPER
                     RIGKEGGMLSYLKKP"
     gene            complement(25927..26214)
                     /locus_tag="PK224_14920"
     CDS             complement(25927..26214)
                     /locus_tag="PK224_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17086.1"
                     /translation="MKNTKGTGLLKTTVYCMQQEFLRHLVKIAGLQEITLWRTKKKEW
                     NLSVKVKNRPQVLYLSTRREPQLPRAFKRLDAAVAAGQRLCGSRQFKLVLL"
     gene            complement(26522..26782)
                     /locus_tag="PK224_14925"
     CDS             complement(26522..26782)
                     /locus_tag="PK224_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17087.1"
                     /translation="MVGADARGGIQEDSRFEDFAGMDDAECQGADDGVFGVETANEEL
                     LAVEPAKELQRRSLVGLPCQQIVEACREGIKEPVFRRVVQSV"
     gene            27172..28080
                     /locus_tag="PK224_14930"
     CDS             27172..28080
                     /locus_tag="PK224_14930"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HRB17088.1"
                     /translation="MAKQPTRQDIYTRVTSQISLQLEKGVRPWIKPWSVEHTAGRITR
                     PRRHNAIPYRGVNVLLLWSEALAKGYAAPLWMTYKQSEEMGAQVRKGEHGSLVVFADR
                     ITKTETDSAGQDVEREIPFMKGYTVFNVEQIDGLPAHYYAQPVNPLPLSDRLTQADQF
                     VTATGAAIHLGGNQAYYSPATDRIQLPPFEAFKDKESYYATALHELTHWTRHENRLDR
                     DFGRQRFGDAGYAREELVAELGSAFLCADLGITPEIREDHADYIGHWLKVLAEDKHAI
                     FQAAAHAQRAADYLQQLQPPPVQHAS"
     gene            28657..28896
                     /locus_tag="PK224_14935"
     CDS             28657..28896
                     /locus_tag="PK224_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17089.1"
                     /translation="MATPSNTPKPVQSFTCGTVQAVIWKNTGKKGEFYSVTLGRSYKD
                     AEEKWHTSDSFGVNDLSAVNTVLEHAEAWLRDHSK"
     gene            28979..29398
                     /locus_tag="PK224_14940"
     CDS             28979..29398
                     /locus_tag="PK224_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17090.1"
                     /translation="MTDYQNFATTIATLLGGRQSVRITSGNYMALCIESIGHSAEGNP
                     LISLAHYGVQNGDLMADPEMTFEVYATPSPAAEPLTYRNDYMGMMQEVYEYSPSGKKS
                     HIRPQLKKDLRHFAALWFRNLKTQGFLSESAVREIRS"
     gene            29559..29897
                     /locus_tag="PK224_14945"
     CDS             29559..29897
                     /locus_tag="PK224_14945"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB17091.1"
                     /translation="MPFVLRPYRRFPVQCSVTYNAGPFQGHGTVWNLSCAGWRLSGDL
                     PMRPGETISLTITLPNEQRIEVPEAVVRWSRGEEFAVENIAIEPHTQMRLQHYVKRLV
                     QEPAGAVVRD"
     gene            29955..30257
                     /locus_tag="PK224_14950"
     CDS             29955..30257
                     /locus_tag="PK224_14950"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRB17092.1"
                     /translation="MGLKLVRLALGIGLGTILLFALPLFLSTVTDSSEVYAAEPLDLN
                     TATADQLKALPGIGEAYSEKIIKARPYKRKDELVQKKIIPQATYDKIKDQIVAKQR"
     gene            30809..31303
                     /locus_tag="PK224_14955"
     CDS             30809..31303
                     /locus_tag="PK224_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRB17093.1"
                     /translation="MVLPKRQQLPRLGILTLAFLLIANTAFANFSGPVVSVLDGDTLE
                     VLHNQHPERIRLSGIDCPEKKQAFGQRAKQAASALAFGKEVTVQTHGHDKYRRTIGEV
                     TLPDGMHLNQELVKQGWCWWYRKYAPRDTVLEQLETDAREGRKGLWADPQPVPPWEWR
                     KKSR"
BASE COUNT         6887 a         9003 c         7996 g         7474 t
ORIGIN      
        1 acccttagcc gaagtgcacg accgacggct atcagggggt ccaatcgaat gacgacgcca
       61 tcgggatccc acggggtgta aaagaaagaa cgttcacagc agcacttgcg ccttaaggta
      121 gtgccgaagg cgtgatttga tcggagccaa ttcagtaccc tcaacgctga cgggcgtttc
      181 gcgcgttttg tgctagaaag tcaacgagtt gggcggtgag aaggagtgag attgagtgag
      241 acggaaggag cggaaaatcg ctctgctata cgaccgtttt acgaccggtc gatcaacgaa
      301 ccaggagaac atagcttccg tctcgttcat tatttcactg gcaccgatca ggctattgat
      361 cgcctttatt accaatactg tcgtcagact cgttgccgtc ttcttcatag tccctcttcc
      421 gcatttctct tctagactca gcgcggcgct gttctgatgc gatcatgtgg tcgagttctg
      481 caatatgctt ctcggcaaac gctttgacta ctggccgttc gtctgtcagc cattccctca
      541 gggactcctt cctagcttgc cacgcctccg cgagcccaaa ttccccagag gtcacaccga
      601 cgttatcaat actgacccgg acctcgtcaa gtttgtcctg gtcatctgga gacctcgcca
      661 caatttcctt caatacaacg tggctggata tcgcaccatc atagttctgg agaatcgcca
      721 aagcgaattc tgcttccgta tcactaccag cctttaccaa gtcggaaagt gcagtagaga
      781 actcttgggt gcagttggga aacgtgttgc taaggagtcg tccacctcga aactggaaaa
      841 gggttcggtc ttgagcgaac cacgatagcc ctttgctgat tgcgagatgc gggttcttcg
      901 aaagtgcatt ttcaaggcca tggaactgaa atgggaccgc ctcgaagtgc tcttcgtcgt
      961 tgacgtggtc accgtccgcg acttcccggg caagccgagc tccaaaatag tcccagaccg
     1021 ccgctggctg atgctcggca agccggacca agatacgctc ggcttgatag tttatcttgc
     1081 gaaggtagcc aagattcctc aggagctgcg ccgtcctctc aggggtcaat tcctcataaa
     1141 actttgtcgg cttctgcaga aaccacgctt gcgagaccca gcggggatct ttgcaatcgt
     1201 tcaaaaaggt tagagcatct ctcacaaatg tgtccgcgtc aggaactttt tctgttccgt
     1261 aatactccaa agcgaggagt agacagtcgg tgacagcccc ttgatcgcca ttttcaattg
     1321 cgcgtttcag caggcgattg gcaaagtctg gcttcttaac atcggactta cgaaggtgcc
     1381 aggctatgct cgtgaggttt ctcgccgatt caagttcact ctccaagatc cgctcataga
     1441 tatcagctcg gctacccaca gcgagtccgt tgaggaagcc cggaaggaag gtgcgtaggt
     1501 catccgaggc ccgtgctaaa agcctgtcag ctacttcggg cttactctca gccagcttgc
     1561 tgatgaaatt tccgaaaacc ggaaacgtgg caagatcgat cgacttagtt tcggcgcaac
     1621 gttcaatcag gttgaaccaa tcgttctcat tctccggatt gatctcatcg atgtagcgga
     1681 tagcttctct acgccgatat tggtcagctc cttctagttc gaactcctcg tcggtccagt
     1741 gatctggata aacagattca aaccctacga ggactttata tcggacaaac cgatcatcgg
     1801 tgttgattat gtctcgaaac tttaagatgg cggccatgag taccttggca tcggtctgac
     1861 aaccaaaccg attgtccgtg tcttctgtaa gaccgttcgc cctgcgatag tcgtataaga
     1921 aacggtgttc caggtgctgc aatacctcgt agctcgtgtt cttaacacgt tctgttaaga
     1981 attcgacaat gcgtgtggca tccctgagcg tgatagccag gagttgatta gaatattgcc
     2041 cctgatgggg agtccttgtt gcagcaccca aggcagaaag aaccgcgcgc ttttgcacat
     2101 cgagggtcga cctatcatat gctgcgaaaa gggccttgag cgccttgtcg cgaacctctt
     2161 tgagctgatc ggatgcgggc agggcgcctg tactcaagac cacggagtct gctttccact
     2221 tttctccggt gatgtctgat tgaattgctt cggtccagac ggtaagggcg atgggccaaa
     2281 cactatccac ctcggcatcg ttcttgttgg caatgtaatc taccaatgcc atctggagca
     2341 tcggccctac cttgttgtag acgtcgatct tgtactcgga cagttttttg acagcgttca
     2401 caatctgttg gcgaatagca tcgtttggct cgtcccggta aatgtcgatg agcagttgca
     2461 gcgttccggt tacatcggcg tagcgcaggt tctcaacgat ctctaccaca aggtttgtca
     2521 ctgcttcggt cttgacatct atttttccgt cggtaatttc tatcggaatg ggctccgtgg
     2581 tgcgtgcacc tttgactgct ctcaacaaag ggcctttcaa tccctcaaag gcagctggca
     2641 ggtcgtcgta gatggggatg gcataatctt ttagaccgga cagaatatcg taacgctcgt
     2701 cattattctg tgctttatcc agtatgttgg cgacgttttt gtcgaatagt tctttacctt
     2761 ggagaacccg ctcatactcc tgctgggcct tttgaagctc atatccagct ggaaccagat
     2821 acttatccgc gatccgatca aatctatgct tgataccttc cagagctctc gcgccgaaat
     2881 ccttacccat cttgttcttg tatagaatgt cgtgggaggt ctctgaccaa gcgtgctcca
     2941 gaatggtttg aacttgaatt tcgcaccgta ggccagcaaa cttcgcatat tctggaaggc
     3001 gtagccgatc ctcggaaagc cggactgtgt agtgaattcc acggtattgt gctcgcggat
     3061 tttccggagt cgggtggtga accttagtcg aatccggctc gatctcgaaa ttatcgtgga
     3121 ttagcgatga ctgcacgaag cggttaacat cattatttgt gtagaagatt aggcgagttc
     3181 cagcaaggtc acgccgatcg cgttccagcg tctccgtgtc cagtctgcct tcctcggcca
     3241 ggcgacgccg caggttttca atctctttag cacgatactg gattgactga ggccgaggca
     3301 gactctcagc ggcctgaatg gcctttttca agatgaagcg gaccgtctca gcgaaggctt
     3361 tgtagatcga aaaatatttc gtctcgtagt cgttaaggtt cacgacactc agatctcact
     3421 ttcaatctca ttcagcattc ccaccgaagc gagcggagga gcgtccgtac gcctggaact
     3481 aatgcagcgc tccctgatgg ctagagtgcc gtatgagtca tgtcggcttc tggtcgataa
     3541 gggacatcaa atgaagagtc gccgcgtggc gttcaatgcc gctaactatt gagtgtactg
     3601 accggcatag taccgttgta actgcgagtg tcacaactct ggcacttaag atttgtgcat
     3661 tcatgtcatg ggcttggaca tatactgctg gatacggtgt tgttggggca gacaaacaaa
     3721 tcccatttag catacaacgg aggcctgctg atactccaca gctgtacttc aatgatctga
     3781 tttatggacg acgcaaatca gttcttcagt cacatgcgtt cgagaactta cgggaggaca
     3841 tgtgggcagg ttttgtggac accgacacgc gtcttaggta actgttctac ggccctcccc
     3901 ggcagactca gacccaatcc agccaagcgt agcgcgaagt aggcaccatc tcgaatgaac
     3961 ggccgcttcc cggcgtggag attgccgtag tgactgtcgc caagtggccg atggcagtcc
     4021 tcgacccaaa ccagaagtcc acgaccgacc gctatctggc tgctcccggt ttcattcaca
     4081 cgcctaactc gctgtctttc aactcggcag cagcccagtt gttccagtag ttcgtctggc
     4141 atgactcggt cgctcagcgg cgctttttct tggcgggctt ccaggtcttt ggatcgatgt
     4201 tgtagaaggt gccagccttg gaaccttcat tgaaccgttc gtttgtgagt acctcgccgt
     4261 tggccgaccc acacgagggg cacttctttt cggttgtgcc aatcaggggc attactttgt
     4321 ggcagttgaa gcagttgtag aacacaatag agaagtccct tcaagacgct caactattga
     4381 atatgctgat cggcatagcg ccatgatagc tgcgaggagt tgaaagcctg gatcagcgca
     4441 tcctcggggc tacgcactcg tctccatctc gattcagaag atgcgttgag atcataagca
     4501 gggcgtaggc cgcccgatcc ggcgccagcc ggacgggcgg gaaagttccc cgtctcagta
     4561 acacgggggg ctatggatag ataagagatg gtgaaagtaa cattgggcga catgtttagc
     4621 taatccagac tccgtgatgc tttcgtgaca tgcctcgtgc tacagaatcg aagacccctc
     4681 atcaggaatc atggggacgc gtatggttcg aaacatactt gtgattgagg atgaccgaga
     4741 tatcgcccgt ctcgtagagc tccatcttca tgatctgggc tacacagtaa agatcactca
     4801 tgatggagtc gaaggtctca gggaagcgct cttgacgtcg tacgatctga tgatacttga
     4861 tgtgatgctt ccgggaatgg aaggattgga gttatgccga cgcatacggg ccaagtcgac
     4921 ctataccccc atcctgatgt tgacggcgaa gtcctctgaa ctggataggg tgcttggatt
     4981 agaggtagga gccgacgact acttgacgaa accattcagt attcgggagc tgttggcccg
     5041 ggtcaaggcg ctattccggc gggctgaagc ctttgcgagc gaaagcctcc cgaaaatggc
     5101 aaacactatt atcgcggggg atctccaggt taatgtcgaa aagagaaccg tgaccctgag
     5161 aggtaacgcg aaggacctca ctgcaaaaga attcgatctg cttgtccatt tcgctgtcca
     5221 ccctggccga gtctacacgc gcgcccaact cctcgactca gtgtggggct atgggcatga
     5281 cgggtacgag cataccgtca actcccatat caaccggctc agggcaaaga ttgagcaaga
     5341 tccggcccgc ccggactata ttctgactgt ttggggcatt ggctataagt ttcgcgacgt
     5401 cgaggatgtc gaacgaaggg gctagtcgat gctgaggacg ttatatggaa agtttgcggc
     5461 tgtccttctc atactctttt gttcgatcgg ggtcctatac atcttcctca cactctttac
     5521 gacacggatg tatctgcagg aggtgaacca gaaactcaac cgtgcgattg cgaagaattt
     5581 ggtggcggac aggcttctgt cgattcaagg ggaggtcaac ccgtacgctc tgaaagaatt
     5641 gttccatgta ctcatggtcg tcaatcccaa catcgagatc tatctgctcg attcgaaggg
     5701 aaccattctg acgttctcag ccccgcccga aaaagtaaaa cgctcgaaga tctcgctaga
     5761 acctgtgcat agatttctga gggaaacgga cgcttttccc atcgtcggcg acgatccgcg
     5821 tgatgtcacc aggcaaaagg tgttctccgt ttctcctttt ccgttgcacg gtgatccaga
     5881 aggatatctc tatattgtgc ttggagggga ggaatatgac tcggctgtca ctatgctaca
     5941 aggtagttac atcctgcgat taggcctctg ggcagcactg acgggattgt tgtttgctct
     6001 tctcgcgggg ttgttgctgt ttaactggct gacccgccga gtggggcaac ttgcttccac
     6061 tgtagagtca ttcgcaaaaa gcgatttttc tgagcagatg caggcgaccc tgtccatcgc
     6121 gcttcccagt cggaaccgcg acgagatcga ccagctcggg gggacgttca atttgatggc
     6181 gcagcggatc ctgcagcaaa tgacgaccct taagcatacg gataccctcc gccgtgaatt
     6241 ggtcgccaat gtatcgcacg atctgcgcac gccccttgcg tcgcttcacg gatatcttga
     6301 aaccctcctg atcaaggagg gcacgctgag tgcggaggag cagcggagtt tcttagacat
     6361 tgcccttagg caaagtgagc gattgaggcg actcgtaggt gaactgtttg aattggctac
     6421 gctggattca cacgagaccc aggttcattg cgagatgttc tctctggcag aactcgttca
     6481 ggatgtggct cagaaattcc aactcattgc gaaacaaaag caggtcgaac tacacactca
     6541 gttccatgct gatcttccct tcgtgtgggg agatatcgga ctcatcgagc gcgtgctaga
     6601 aaacctgatc gaaaatgccc tccggtacac cccctcggga ggcacggtca ccctagctct
     6661 gagccagcag gagcaccagg tccttgtgag gattacggat actggctgcg gcatcccaga
     6721 gcaggacctg ccatacatct tcgatcgatt ctatcgtgtt gggcggcatc aacgatcaga
     6781 gggtgcgggc cttggtctcg ccattaccaa gagaattcta gaactacacg ggagctgggt
     6841 cgaagtagac agttcggtga aggtcgggac cacgattgcc ttctgccttt ccacctcttc
     6901 ctaatgaatc tggatcgaac gccgggtcct tcctgagttt ctagcccctc ctcagtctac
     6961 ggtgaggcca accgggacgg caacctgcct attgctgaat gaaggaaagt aacggatcat
     7021 agagcagggt cgtgatcgat tcgtgatctt ttcgtgaacg agacgtgatg agcagggtct
     7081 acaattttac aaagggtggt accgcgacag gtaccatgca gctcttcaca tcgatcagaa
     7141 ggagaaacga atgcgaaaca gattgttctt cgccatctta ggatttatgg tcattttcgc
     7201 acaagttgcc ggtgctgagg tggatcgaga gaagctctta aaggatcctg gagtatatgc
     7261 tacctttgcc gtcttcaagg tcggggatca atgggggcaa ttggataagc aagcgaaagc
     7321 cattgctgta tccgaagtga agaaggtgtt tcagcagcat tcagacaaga ttactgttga
     7381 tgctcacttg ctgcgtggcc tgtccgagaa ggcggacttt ttcctcaggg tccattccaa
     7441 agagatgcta cacaaccaga attttctgct ggactttatg ggtacgactt tcgggaaagc
     7501 tctgaagaac accgacacgt tcaacgggat caccaaggcg ctgaattatg tgccgaactt
     7561 tcctgaagac atgaaggccg agctgaagac ccctccgccc cagggcagtc cgtatgtggt
     7621 ggttgtccca atccgaaaag atgccgactg gtggctcatg tcacaagatg aacgaaccgc
     7681 gatgatgaag gagcatacgg atccaacggt gggttacctt aagaccgtca agcgcaaact
     7741 ctatcattct agcggcttgg acgatctaga ctttatcacc tatttcgaga cagctaagct
     7801 ggacgacttt aataatcttg tgattgggct attgaaggtg aaagagaatc ggcacaacaa
     7861 gcggttcggg gatcccttgc tgttgggcac cattcgcccg ctggaggaga ttctggaaat
     7921 cctggcgcgg tagtgcagga cttccgtttc gatggttagg cggatgatac ccatgtgaaa
     7981 ctgtacggcc tgcatactgt atcgaacagg gtggagtttt cgcaccgtga agattctttg
     8041 cgtaaccttc gccggcgaat acgccgagtt tcaaacatat tcccaagtct catacttctt
     8101 cactttctca gggctacgcc gacgtgcaag gaccacacgt cggcgtagtt ccgtttccca
     8161 tgctccgagc gcactactgc ttggggcata atttcaggaa gttcttttga ttgacagccg
     8221 tttgtagcgc ggtctttgcc tgcaacgccg catactttgc caacgctgca gaacattcac
     8281 tgggggcatt ggagcccgcc gctaggcata acatggcttc gcatgcgagg cgcgtatcgc
     8341 ctgttaagac ttgtgcctgt gcttgcggtg tcatcgatgt ctgcaccaac aacagcagcg
     8401 ccgacgcgga caaaatggcc ttcgtcgttc tcattgtcat ttcctcaccc cctttctgtt
     8461 ctggctcatg ctcctccggt ctggtgtcct ggccttagtc atgactaaga ccaggctatc
     8521 agcggtcccg atcgtgagga gacacccgct ctttgagcgg ttccacaatc cgtcgaaaca
     8581 cgtcttgaat caatgtcggg tcgccttttc tcgtcacagt cggatcatcc gcctgtcgtt
     8641 gtggggtgct tctcgatgcc gcccgattcc actcgtccgg tctgaccttg gcatagtcca
     8701 cggtgtagtt ggcctcatct gccagttgta cgagcagggt ttgcgtgctc cgaccattcc
     8761 cctctggaaa ggggtgcatg tcgttcaacg tcacatacgt tgtggcaagt agggttgtaa
     8821 aatccttctg gttcagctcc ttcaaattct tccattcggc caggccctcg cgaatcgagg
     8881 ttgacacatc gtgaatcgtc ttcgccggag caaacgtgct gacccaccgg ggatcttcag
     8941 ggtccggctt gacgacattc atcgttcgtg tttgtccggc ccactcgtac acgtcggaaa
     9001 ataggtgctt gtgaatcgct ttgagatggt ccaaatcaaa cgtgccggca acgggcgtta
     9061 cggtcaactc cgcgatcctc agattcgtga tgttgtactc cgcccgcgca agatccgtgg
     9121 cactcatgat cccgagctta ttgacgagca cgcctgtttg gaggtcgcgg tagggctcac
     9181 tcactggcgt cttctcgagc gggtctgttc ttcaagaatc gtcacgctca cagccacacc
     9241 aaacgacatt cgaccatcga tgacggcctc ctggagtccg tccatctctg gcggcttctc
     9301 catgccctca agtgcgagaa tggcatcggc ttgtctgaag gcttcgcgcc gcgtgtcctc
     9361 aacattcata ccgtcgtcct ttcttgttcg tccattctgt ctagccgaaa tcgccgacta
     9421 tttgttatac taagtacact gattcttgag gtcagttgta cggagcgatg ctcgattcat
     9481 gatgctgatg cgacgacact cgtatcccgg ttggctggca tctctgcgcc atagcctcac
     9541 gttcgagcag cacgccgtgg cctccgctgt gcccgcacag cccggcacgt gctcgacacg
     9601 gtctgcgctc gacacgtcgg acatcggtgc gtaccttctc atcatcgtag tgttagctct
     9661 ccacgtgtag cgagagagcc aacaccacat gttttcggag gttttatgaa gacgccagcc
     9721 aacccgttta ccattcacgt cattgacgct catgatgtcg tcccatacat cgcggcccca
     9781 cggccctcga tcgtgaatcg tctacggccg tacatcacca atgctttgac cagaacaggg
     9841 cggggcttgg tgcaaaccag ccgagtggca gtcttcgtgg tcactgaact gtcccgtatg
     9901 atcgttggtg ccggaatcgg cgctgtgaca ggccttcgat tgctctattc cttcttggct
     9961 gcgatgctgg aattcttttt tatcaagctc ccagtatttg tcattggggt agtctttttg
    10021 ctcgtcacga ttgctttctc tttgggaatc ctggacgcaa tgtatcgtgc cgttctcgcc
    10081 caccactgat gatcccttct tctcctcatc ccctcttcga ggacctgggc cagcggcagc
    10141 ccctcccgat tgtctacaca cacgttgcat tgttaccttg aatctcatgg ggtctgactt
    10201 cctcagcagc cgtcacacgt aatgaggtct atcgatggct cgcgctcaat gatgtatcgc
    10261 gatcgccccg ccataaatac ttggatctcg ttgccgagaa tcagacaggt ctccccgtct
    10321 accggcgcga gaaatcgagc gtgaaaatcc cctgataacg cgaggcattc ccagaactgg
    10381 gttttcagcg actccgtagt cggatgctcc tgaagccaat cggccacaat ggcaatggca
    10441 agatccgccg aacctggcct agaggaatcc agctcgaatc ccgttttact atgaaacggg
    10501 atatgctgaa gccgatagcc tgtacctcgc tcctcaacct ccacctggat taactccgtg
    10561 tctgaatctc gaccgatacg atactgtttc atgaatggct ttgaatcctg aaccccaaca
    10621 acaggaggac ggtaagatgt gccgggtaat agatatagaa gaaccgtgat gctctcggaa
    10681 tctcgatctt gacgaaagag gccatcccag cgaggacagc aaacgacgcg caggcccacc
    10741 aattgccgtt gatcagcgtg agactggctg tcccggccag ccacagtagc agtgctcccc
    10801 atgtcggttg tttccagtac aaccaagcac ccacgcagag cgtgacaccc ggccaccaaa
    10861 attcaacgaa ggccccaccc aagagaaaca cgaccgccgc tttggtcttc cagaacggtc
    10921 gccgtgactc gatcagatac atgatgctca ccgcgacggc gagcgttccc atgcagttga
    10981 gcggccacca cccaaaccca agccccccta atgcgataaa cgggaccgag gcgatcgcac
    11041 agcagaagag cagacgagtc aggacccgga cgtacaccgc cagattctgg ctccggacgc
    11101 gagccaggtt gtaggccagc acgattccaa agagcggcaa ggccaatcgt cccgcctcat
    11161 agatcccaga cacggcggga atcatgccgt acttctgggc atgatccgca gtcatcagga
    11221 ccagcccgag ccacttcatg gcttccaccg cgccgtctga caactccaca cgaggcaacg
    11281 tcaaggactg catcatcgat taccgtccgt ctctcgtgcg ctcctgttca cgggttcgcg
    11341 ccgcctctgg ggcgagatca gtacgagccg ctccgcgatc gaatacctgc acgacgggct
    11401 catgggtcgc cactcgcgcg gcagcggcga ccttttcgcg ctcgccgatt ccgagtgtgt
    11461 gtaagttgac gacctcccac atattgcgct ttgcgggttg aaacgacgtg cctacgtgtt
    11521 ctccggcctc atccctgatc gggacttgga cggtcacgcg ctcatgcgct cgctggatga
    11581 gcgccactgc gtcacctggt ttcgcatcgc tccgttcaat cgctctggcg agatccaccc
    11641 cccagatttc tttctctccc tttgccgtcg ccactctcac gaaataactt ttctcggact
    11701 ttgggtcatg atcgtaatga tcaatcccat gttctacgac tttgccgaca accagacgct
    11761 cgccgtggag tcgcgctttg gcttcctcca tcgccgccgc gatcatggcg ggcgagtcgc
    11821 cgcgctcgcg caagatcgtg tgaagtgtgg ccaccgccac attctgagca gccgtcaacg
    11881 agtcaggctc tttttcactc cgattggttg aacggtcgcg aggcccgacc tgttcaaagc
    11941 ggttctccgg ttgtcgcgtt ggggtctctc ccttgcgctc ctctagtcgc gctcggtcga
    12001 tctcacgcgg tttgtaccct tcgacctcga tgcccgcaat cgtggcttga atccatgcct
    12061 ctgctttgaa ttcatccgat cctttcacgc ggagagacgt ccaccctttt tcctgagccc
    12121 ggagtaccat gccgtgaatt acggtaggat catcatggtc ggtggtcagg cgtttcccgt
    12181 gatcggaaaa cgccacttgg tctggtgcac cggagccacc acgaaagtaa aacttgttgt
    12241 cggcttcaag gaagcgccgc cgtaatgatt cggcggcttc cgcgctttga ttcaataacg
    12301 gttcaggctt gagagttcct cgttgaccct cccgagggag aaccgtctcc ttcgtgctgt
    12361 ctttctctat cccctcacgc tcaagcaatt cattcatcgg caatcctcct ttaactcttc
    12421 cattggggcc atacactcgc cggttcaata tccataaatt caatttctac acgtcgattc
    12481 tttgcccgtc cttcggctgt cgcattgtcg gcaatgtgtt cgccgatcgg gaacactttg
    12541 acatcgatat tcatgggcgc aaatccctga tcaatgaggt accgtctgac tttttccgcc
    12601 cggtcacggg cggtcttccg ttcaaaccag acgtcttcct ctccatcggt gtacccgcgc
    12661 acttgaatac attggccgag catgattgct ttcgtcagat acgctttcat tgattcggtg
    12721 ggtacaaagt ccgagaggcc tttgccttct gaaacccgga agagcaccgt gctgttgtgg
    12781 agttccaatt cttcccgctc actcagcttg accgacatcg gctcacgaaa ctcctcttct
    12841 ggcgctctgc ggattgctgc cggaggtgtt ccggcacggt ccacgctttg gagggaggcc
    12901 agttctacga ctcgggtagt ctgacgtggg accgatccac ccgtgcggat tcgcgacccc
    12961 tggccgctgt ccgcccggtc ggttggcccc tcttcggtcg gcatcatcgg cgggacagga
    13021 acatcactcc gcacggctgc gatcggaggc acagccaagg ctagcggcgt ctccttttgg
    13081 ttcgccgtgg ccttatcagg gacttcactg agcaatggag gacttccggc gccgacattc
    13141 cgtacctggc gcagacggcc cttgttcgat tcctgttgct gaagttgtaa gagcgtcacg
    13201 taggccttca actcctgaat ctgctttgtc agcgcgtcca cttgacgcgc gagcccgttt
    13261 cgctccactc ggtctcgctg atcgcccttc acccgttcgc gatattggct gatgacttcg
    13321 tctgagttca ccggaactcg tgactcacca gacggaataa caggcgtcgg cgcacacgca
    13381 cagaacaacg cactcgcgag gcatacgatc acgccgtgac ctcgactcct cttcatagaa
    13441 tcctcccttt tcctcaactg tctcgttcca ataccgataa atcaaaggcg atgcgcggcg
    13501 acgctaattc cgccacacgg ctctcaaccg tgggatcgac cgtctctgca agacgttcgc
    13561 tcgcctcttg tgccgcacgg ttgaggagtt gacggccgtt cgcggcaggc acattcccgc
    13621 tgccggccgg cacgtccggc tctgtccact ccaactgaga aaaaaacgca tcgacgagct
    13681 gtgtcacatc ttcgacattc ggtgggtcac gttgcaccac agggggcaac agccctgagt
    13741 cgatagtgag tctggcaaga tcaatcggtt cattcggagt caatggacga atgcgccgct
    13801 cgaccttggc cttgtggaga tccacatcaa ggatgggaat gtgtaccgag aggtccttac
    13861 gcacaagggc ggctgtttct aactcagtct gcgtcgggag cttacggcct aacgctgcca
    13921 gagacggact cgcagatttc aagcgattca caaagacggg atcaaggtag aaaaaggcct
    13981 tgttgcagag aatgggtttc gtattgacga gattgataat ctggcgcgtc ggccccaact
    14041 ccttcaattc ttgcggcaac atgagtggac gcgcttgatc ggagatattg gagctggttg
    14101 aggcctgccg tccattgccc catgttctcg gatggctcat acccgttgaa acggctttcg
    14161 cggtataggt ccctagcatg tgtgaatact cgttcgcgtc ctgctgctca cgaggcgcga
    14221 acatcacttg gcaactatga ttggtgataa atgtccgtgc atccttctcg ccgtagatcg
    14281 attgcaattg cgactcactt tgaatgatcg gaaagagccg gatgttatag cccgcgataa
    14341 atccgacact ttttgccaga atatcgactc gcccgattga cggaaactca tcgagaaaca
    14401 agacgcattg atacttcaac gcgggattca ttgcgggaag atcgatcgtg ttgtgatgga
    14461 tcagttgcga gaaaaagaga ttgaccaaca acgccgcatc gctcagacga ttcgccggaa
    14521 tcccaacata gatcgacatg cggcgcttcc gcactgcgct gaggtcaatc tccgtcgcgc
    14581 tggtcgccgc atcgacaatg gggttgctga acatcgtcag gggagcggta aacgtcgcaa
    14641 ggataccagc catcgtgttc tcgcttgtcg tacagaaccg ttgcaaggct ccggtacaat
    14701 cgtcgctcaa ccgctgatcg cccgtaccac gcttggccat gagcccttgc aaatactcct
    14761 tgatgggttg gcccttcccg gacgcttgcc gcagtaattc ccccaagcta cagggcagcg
    14821 tgggcgattc gaggaggtac agacacagac cgagaaacag atttcttgca gactcattcc
    14881 agaacgcatc tttgacgttt tcggcaggat agagaacatg cccgatcgcc tggatctctc
    14941 ccacgcgcag attgggatct cgatgcaccg catcgagcgg attccatcga tggcttcggt
    15001 tatccgtgcc aaacgggtta aacagaaata cctcctgccc gtgcatctgc cgaaacttgg
    15061 aggtataggc gaagttttcc attttgatat cgagcaccac caccgactca ttaaagttga
    15121 gtaaattcgg cagaacaata ctgacgcctt tccccgatcg tgtcggcgca gccaggagca
    15181 caaattcttg accgccatag atcaagtacc gccgtccgta cttgccaaca atcacccctt
    15241 tgtctccata caatcccgcc ccggcaattt ccgttgggct ggcaaaccgc gcgtcaccgt
    15301 gtaacgacct cgcgcgggtc atcaccgaca gcacgagcac gagcggaaga ccaaatagcg
    15361 cgaacaggcc catcccggct gagagttgca accgctttcg ttgggttgga tcttcgctat
    15421 acgcccgcca ggaatccggc caggtgtcta aggacacttc ttcagggacg agcttgttcg
    15481 tcacatagaa catggccccg gcgatgtaat ccgcacccaa gaggcccagg gggatgtaca
    15541 agagcgctgc cagcactccg gcaacaattt tcagtcgcat acgatcctca agcccgaacc
    15601 agtgtggccg ctccgaagcg ggccacgagt tttgcgatcg ggtcataatg gacttcggcc
    15661 acatagcgct ctttgtgagt cggttgccct tctgcgttat agtggttgtg cgcgacgacg
    15721 tgtaaaatca cgtcgatcgt taaggacacg agtcgcttga gtgtcgccgc atcgtagatg
    15781 gcggcttgct catgctcctt acacatgaag acataccgtt ccgccgccag cgcacaggac
    15841 tccgcatgat aagacgtgat cgagccactg tgacccgtcg tcaacaactt caagaaatcg
    15901 aaggcttccc ctcctcgcaa ctcagccaat aacacccggt ccggcttcat ccgcatattc
    15961 gaggcgatta aatccgatgg cgtcactttc gccacgcctt gaccgccttt tgagtacaac
    16021 aggtgcacac gattcccatg attcggcaaa aacagttcgc gcacatcctc aatcgtaatg
    16081 agccgttcgt cggctgggat ggactgacag atggatttca tcaacgtggt ttttcctgag
    16141 ccggtatctc ccaccacggc aatattcttt ttcgcccgca ccgcctgttg aataaaatcc
    16201 cccaggcgtt tcgagtcgag gcaatcaacc aagctacgtt ctaccttatc gagatccccc
    16261 atccgtgcat ccagctcact cgatcgcgcc cacacatagc gattgaacgc ctcttctttc
    16321 tcgtactcgt ccagtgattt aatccaggcg cttggccgac ggatcgagaa tgaaacggta
    16381 cctaattcga cggcgggagg tagcacaatc tggactcgtt ccccatcggg aagcatggcc
    16441 gacaggattg gtttttgtgg cccgatttct tgttcggtat aggtggcaat ggctgtcgcg
    16501 agggccatga gttggctaca cgtcaggtcc ggcgcgtcaa accgttccca cgtcgctccg
    16561 acttcgacat agacctcacc ggggcgattg atcacaattt ctgtggcccc tggctgcgac
    16621 agaaatctga gcaacggccg caacagttcg cgcaccatcg tatcgtgcgc cagtaaatta
    16681 ttcggcgtgg agtgcataga cagttccaaa gtccaagtca cgggccacga agatcgtcgc
    16741 tcgatctccc tggttcttgt agagcgtcgg cttaatgtta atggtcgatt gcaacacgag
    16801 ttcggccatc cgatttcccg tcgtcgctga attttgatag atggatagtt gtccttgtcc
    16861 tccaccgccc gccgcttgcg ccgtcgcata tccaatggcg tcttgcacga tcgagaggag
    16921 aaatgcggcc cctaaacggt cccaccagtg attatccacg tacccggcaa ggcccgatgt
    16981 gcccaattca tcggcggctg gtgagttgag attgatcact actccgttcg gggtcttaat
    17041 ccgtgtccag aggaggaata gccgacgttg tccctgtgcc atcgtcgccg aatattcgcc
    17101 caccgcttca gacccacgat ctaggagcac cacacggccg ttatcgctat agacatcgga
    17161 attgagcaca caggtcgcca tgccagccac ctcattgacg acacggacgg tcaacgcaca
    17221 atcaatgctt ttccctttcg gaagaatcat gttccgatcc cctaacatgc cagcctggac
    17281 tttcggggtt gaggagggtg tgagtaatcc accaattgaa ccggccccgc ttggtgctgg
    17341 gcctggtgcg agcggcactc caacacccct cattccgccc attgttggca gactagagcc
    17401 tcctactggt ccagccggta tagaatcaga cgccgtagta gacacgtctc cggccggaat
    17461 gctcatgcca acaggaatcg cttgtgctcc cccgatcgtg gaacctgctt gatccatcga
    17521 ctcagagcca cgagggttgc caagcagatc gcggactaat gaaatggcgg cttcggctcc
    17581 cgtggcttgt gcgtgctgtg ttcgtgggcc gcttggcaca tttgaactgg cgagaatcgc
    17641 atctcctcca aatcgactcg gttgctgtgt gcctggttgc gttccccctc cttttccgct
    17701 cccgtttata agtggaattg gacgaccgat ctcattcggt ccattctcca cccctggaac
    17761 gatcatccca ggagtgaggg acgccgcttg agatcctccc ttgctcgctg tggaggaccc
    17821 cttgttaggc tgatacacgg gatctgaatc gaagacacgt ttcggcccga cttgcgccgg
    17881 tttattctcg atcttcgccg cttgctcctc tttcgaggct tcggcctttc gattggcttt
    17941 ccatttgttc atgcccataa gcaggccgat caacaccacc gaacccagta tcacgacaaa
    18001 cacgattttc cctgccgtat gtcccgccgc acccgatcga ttattgaccg agacaatccc
    18061 tagctcttct ttcccttctg tcatcgtctt ctcttccatc atgcctccct caacgcacca
    18121 cgcggctgac acccgaaacg gtcactccgt ttgcgggagg gacgccgttc atatcgtagg
    18181 catcgttcca cacgccaacg accgcctgac ccaacctcag gacaaagcgc cgacccatcc
    18241 gctgcaccac ggcaaactct ttctccatat gaaaattgag gcgggtttct tcttcggccg
    18301 gagaaatgta aaagatggct ggaatttcac gattggacgg aaatgaaaaa taagtaaacc
    18361 ggccatcatc gaagaccagc gcggggacga tatcatccgc gcccgttaag acttccatcg
    18421 aatatgccca attccggggc tctggcgtaa acgctgcgag ccgatcggcc acggcttgtt
    18481 gatcatcacg ggcctgcttt gtttgatcca aactggcatt gagggccatt aaggtttgtg
    18541 ggttcggcag ttggatcgga tgccggaaga tcacccgata catggcttca tcttctaggc
    18601 catgaccatc ggaaggtttc cgccaccggc ccagcttgtt gtctgcaagg acattaaact
    18661 caaggctgta gtcccgctta tccgtgcgga tttcgaggtt gttatgcgtg gcattgtcct
    18721 tcggcttcac ccacacttga ttggttccca cgtcagccct gatgcaccat tccgcttctg
    18781 acttcgcaca atcacccagg aagcccgttg cagcgatgac gatcttttcg tcttcaccga
    18841 gaacgatgcg cgtgatcaca ccccggcgaa catgaaccgt tgtgacgtca tcttgtttgt
    18901 atgtcacgta gcgcactcgt tgatcgagtt cgccgggctc tgggagttcc gcagcggagg
    18961 cccatacgtt cggcaacacc aacacaacta taaggcacca gaccacccgc atcagtattc
    19021 cgttcatgat caccttccta agagcgttcc atcattgcca cagggacggg cgggctggcc
    19081 gtgccaattt ccgcgtccac tctgtaactc gtcactttgt agccgagcgg gttttgaatg
    19141 agatcctttt cctgcccttt catcgacatg cggtattcat aggcgagtgt ggcgatgaaa
    19201 tactggggaa ggtctgaggc ctctgcttca gtgcgttttg gtgtcttctc aaagcgcact
    19261 gtggccttca gcccgactgc atccgcgttt aacgtcacgc tcaatacctt gaccttcaat
    19321 tccatcccgg aaccgagttt cttgtctcgc gcgtttggtc cgtcgtagat ccttgcgtag
    19381 tcacgcccta cctcatcggc actcatcgac aacaccgtgt cgtaatcggc ttggagcaaa
    19441 cggaaatagt aggactcccg cgcgatgaca tatttctgag tccaatgctt atcgagcaat
    19501 tcttgatacc ccatcacggt acgatcatca gccgcgctga ctaactccac gtttccggtc
    19561 gcccgatcga ccgagaagac atagggaatg gttgatttga gtggcgcgag acaggccagc
    19621 ccgattccca acccaaatgc caacacgcag gcaatcccgg ctacccacca ggcccgccgt
    19681 tcggattgtt cgagccgtat acctttgtct gtttcccaat cacggccttg atcgtaaaac
    19741 gtctgttcgt cgcttgcttt cactgttgtt ggttctgcca tgttcacttc ctcctgaacg
    19801 gttacggagt ccagggtgcc atcgtgtcaa atgctccggg catgccagga acaatcatct
    19861 tgaggactcg ttcgcgttgt tcctgttccg ccgccttttc ttctgcttcc gccgtcgcct
    19921 tcatcgtgat gatgcggttg gtatcgtttt gcacttgcgc ttgctctccc gcaattcttg
    19981 actgaaggtc taacgaggct ttctcgtcag cagtggtatc gatactgttc gtgagtgatc
    20041 gaatttgatt catgcggcct tccaacattt gaaacgtgtt ggcgtagtag gcctgggact
    20101 gcggattttg gttcaagatg ttttggcaga ttcggagggc atcgcctatc ttcccctcac
    20161 agttataaat catgcgattg tttcggataa tttgcgctgc cgtcgtcaag ccgctgatcc
    20221 caccactacg aatcgcgccg tagaccgcgt tgacatctga gggaactaca cccgctaatg
    20281 cgaggttgtt aaacagactg gccatgttcc gcgaaccggt gatagcatcg atttgtgact
    20341 gagcctgcgt aatttgctgc tgcatctgct gcaattgttg aatccaagaa agcgcctgca
    20401 taatggactg caccaggttt gccgcatcga tgacgggaat ccctgcctct gccttcggca
    20461 cagagccaac aaataccatc gcaacaagtg cgagggtcac tagtactcgc ttcatcgcgc
    20521 ccataactcc cccctttggt tatgttgtcc gttgcaaccg ttcaatcacg ccacgttgat
    20581 ataagtccgc ccccgtcgaa ctcgttggag ccgggagccc caaccttgcg ggtgttgttc
    20641 cgttcacggc agtacctttc cctgtaccga taagttctcc tgtggaactt ctgtcaggct
    20701 ctcgcctaag agccctcacg agccccctta atccccctgt ggcaaaagcg gccgcatcac
    20761 caccagggtt ccctaaggaa agggctcctg ctaacccagc tcccaatgat tggatattga
    20821 tcaggacata ggcggcactc gcagataaga caaataggcc gatcacatcg gttaagaccg
    20881 aagtcgtgcc ataggatgta atgatattgg cgcaagctgt tcggaggatg cttgagagca
    20941 tggctactac cgccataatc agtacatttg taaataccgc ccctaatgcg ctcgacagcc
    21001 agctctctgc gtatcgttgc gtgatgggaa acatggcaca aaatatgaat gctggaccta
    21061 cggcaaataa aagagccaga gacaccttgg ccactagcaa aattcctagc gcgacaaagg
    21121 ccattaacaa tcctgccatc gcagaaatga acccggcgat gaagaggcta aattgtggga
    21181 gcacgccggt acttgcttca gtcatgatca tggccgacaa agaataaaac ggatcgatca
    21241 tgttatcgat cgtggcccct acggtcggcc ccgctccgaa tgcaacacca aaggcacctt
    21301 gaatcccatc tagcccatct ttgacgatgt tcctatactc aggtccactc agggctaatc
    21361 ccgcaatgag ggccactcga aaccaccgcc agatgagcgt tgaaacgcta tctttggcct
    21421 cgccccgcat caccgaaaac ccagtccaca tcacatagcc tgtcactgcc gtgacagcaa
    21481 tcggtaccaa ttgcgtaatg acagcatcgc taaaatccac aacataggtg gtcagcgcgt
    21541 tattgacgga attctcaaaa tagctggcta cgcccatcta tacgccttgg tttcggtctt
    21601 aaagatcaca gcgtcgatgt ttctgcacga gataaagcat ctcattgcaa tgtgccggtg
    21661 cgcggtgcca ggcgatactt gatgaaggcc tgttccgcgt tgagacaatt cggtgtcttg
    21721 ccaagctcac ctggattatt tccgcacttc tcacgcatag ccttcatctc cggcagatgc
    21781 gctaagtacc aatcgactgt ttgaaccggc tctgttgctt cattcgtgca gccggccgcg
    21841 atggcaacta ccagcacgag cacccatctt gatatctgtc ccatgtgctt tctccttttc
    21901 attgtcattt gatgaggcca cgtcgagtcg cgatacgttg atgcagaagt ggcatccagg
    21961 cttctggggc atcgcctact tcgtcacgga tactatcgag taacgccaca ttttccgtgg
    22021 tgccggagat gatattgaga atgtcgggca tactgcccaa atcaaatctg acaatcgctg
    22081 acccgtgccc ttgcttcacc agaaacattc gactcacctc tccaagattc ttgataatgc
    22141 gatactctga ttcggtgact ttgaagccat ggacataatc gtcgtgatcg gctcggggat
    22201 tcggtaagaa aatctgggtc acgctttgtt ccaccatcgt ctttccgatc ggatgcccca
    22261 gcacgtcgga cggcgattgt gtgacaaaca ctccaagccc agactgtttc cgaatcgttt
    22321 tttgtttgtt gagggcaaac tccgagaaca tcgggtcttg caacggcttc cagaattcct
    22381 ccataaagta aatgaacggg tcgccggtaa tcaggctctc cgtaatgtgg agcagatacg
    22441 ccatgatcgg cgtccggatc tctgggtcgt cgagaaattc tgtatagtcg tagccataca
    22501 acctcgcacc actgaaatct tgtgtatcag tgggattgtc aaaggcccag cccagcggtt
    22561 gtccccgagt ccatttcttc agacgcgctt tcaagctgtt gtcacccgta gccgggagat
    22621 tttgcaggac caatgagaga caccgaacct ctcgggttgc ggcctcgctc atcacggttc
    22681 gcacagcgtg actgatatct tgctgttcct tcgccgagag aatcggggtt tcgtcgccct
    22741 gattcttcac caattgggcc acgagctttt cgcaaaattg caggttgtga gcagtcggtg
    22801 cgaattgaaa gggattaaag cctgtaggca ctcctcgctt cagcggaaaa tacttcccgc
    22861 ccatcgctcg aatcccgatc tccgcgcctc ggtccttatc gaagaacacg caccgaagcc
    22921 cttggtattt cgtcgcgaat gagagcaggc tcatctctaa cgcggttttc cccacgccag
    22981 ttgtgccaca aataaaggtg ttgccaggat acttctcgtc ccgagaatct ttgtcttccg
    23041 gggaagcatg aaagttaaaa tagtacggtt gcccgctcgg cgttttgaat aacgccagcg
    23101 cctcacccca gggattgcca gattgtttgc ctcgcgcaaa attatgaaag gggctcagac
    23161 agacaaagtt ccgactggta atcgtcgcct cgcgaggccg gagattccaa ttccccggaa
    23221 tctgggagta ccacgcgcac tcaggaatca catcgaccgg agacattttg aagcctgatc
    23281 catcttgaaa catggcacgg gtttccgcca tcgacttcgc cactgcattc atcgtctgac
    23341 cgaaaatcgc cacagtgtag tgatattccc caaggtcaat gagtccactt tgtagatctt
    23401 cagccgcctg atccatttgc tcaatttctc gcgtggcggc atcttcggac gcgatcaaat
    23461 ggcctttctg cttgtcgagg gctttaagtg catcccgctt gttgtagatc gaatagcttt
    23521 gcgtttcgat gtactcgtca tctccataga gaatgccgtt attcattcct ggctcggaaa
    23581 actgggggta ctcctgcatg tccagaaacc ccgcatattt ccgttcattc ggatgccaga
    23641 tttccaccat tccggtttta tcgccgaagt ggaggcgcga ggttgggagg taggaggaca
    23701 atggagcacg acgaaagggg atctcctccc acacgccgtt gatgagatac ccaagaaatg
    23761 cgagcatttc tgaatacacg atgtcggttt tcgtataggt acccaggcgc tgaggctcgt
    23821 agcgttttaa gcttgcttct agctgctttg ccgtatcttc aagaatggtg agatgttctg
    23881 tctcccgatc gcgaatctgt tgaaggctgc gcttccccat ccgtttgaag aaactcatca
    23941 ctttcgaggt ggagggacgg tacaccaccg acagatacaa ctccgtcgcc atctgccggt
    24001 gattgtcgaa cgactgataa tatcgatcgt tcaactcttc acaaaatgca ttcggggacg
    24061 tgcccgataa ccgttcttta accacccgcc ggatcttatg ggaccacacc gcataggccc
    24121 caccccctaa tgcacgaatg aaattgtgta acccttcttt ccgcacgaga atatcggccc
    24181 gatccgccgt ttcaaacgtg atcccttcca atcgccagat tgcgagataa tcgcccgtgc
    24241 ccgacaactt gatcacgtgg ggcgtgatgg gggttcccaa cggaatatag tcactggctg
    24301 ggatttgagc cgaggctgct ttggtgactt ctgcgcgagt tctcattgtg tgtttcatgc
    24361 gggatcgggc taactgtgcc ttagcagcac agcagccctt cccgtgatgc tccttagacc
    24421 cgtcgcattt tgaatttcag cgggccgtag gaaatcgcgc cccacgtcgc ccggcttgcc
    24481 aggtgccgca ggcgcatccg cgcccgcatc atcacctggc gaagacgctg atcgtctttt
    24541 tttgtaatct cacgcatcgt caccaccacc ggaatagcca ttaacaccat gaataacgac
    24601 acgtagccac tcacgaaata gaacgaccac accgacagca ggagggtgag accgcaaatt
    24661 aaaatcagcg gtaacgttgg cacaccgccc aacatggcgg gccgtgtgca tcccttgaaa
    24721 attggatcgc gaaagccgtc actcatgtgt ttagttgatg agccaggctg cgatacctgc
    24781 cgcccctccg accaagattc cgccgatcac gacgttgctc acctcggacc attgggcctg
    24841 ttgaaaggcc atcttgaacc cggcccacat aatcgccagg gtgaacagcg tcacggccac
    24901 accgacgagc accgtttgca cgttggtcat caccgtattg accttcgtga tctgcgccat
    24961 tgcaggattg gcctccaaca tcaccgcgaa gcccaacacg aacgatgccg ccttaatcat
    25021 ggccgcactg ccaccgcttg ccttgtttcc gtccatttct acaccatcaa caatgcttgt
    25081 attcatcgct catcctcccc ctggatattg aaaggttaat cacacccgcg aacatccata
    25141 tatgacgtat tggataccat acatgcatct atggcttctt taaataagaa agcatgccgc
    25201 cttctttccc aatcctcctt tccggcgtac tagaaattac gggcaccgcg ctcacaacct
    25261 ttttgacgta gccaagccga tatcccgcag agaaattgcc ggagtagtaa cagctaaacg
    25321 catcgcgcaa cgcccgctgt atgtcctgct tcgtcgcctt cccacccttc gcgcggagat
    25381 aacaatcttt caaaatggcc gacgcgacgc gcagattggt gcaaggatca aaggcttgtt
    25441 ccagagtcac gccatacttt gcgaagttct ttttattcac ttggcccaga ccaacagaat
    25501 agttaaagcc atggcgctcg agcatccgtg ctgtcgcgag ggcctctgcc cgattggtgg
    25561 gttgccattc gagctttcca tcgacaacgc ctatcgcatg aggatttcct gttgattcaa
    25621 cagctatcac ccgaagcatt gtttgcggat ggatttccga tgcacaggtt tgaactattg
    25681 agtagacatc gagcatgaac aggctcccgc atgatcaatc ttggaaccaa accctattac
    25741 tttggtatcc ataggtagga gaatggttta tattcagcct taccaaagat gtcaataggg
    25801 aaacctaaac atcgtggcat tattccaaag tattttatgt ggatgcccaa aaatgaatgt
    25861 ttggtatgcc tgccattgta ggactcgtcc tacaattaat agatgggaca aggtgagaga
    25921 ggctgcttag agcagaacaa gcttgaactg acgtgagccg cacaatcgtt gccctgcagc
    25981 tacggcggca tctaggcgct tgaaggctcg aggcaattgt ggctcacggc gcgtgctgag
    26041 atataagact tgcgggcgat tctttacttt cacgctgaga ttccactctt ttttcttcgt
    26101 tcgccaaagc gtgatctctt ggagccctgc aatcttcaca agatgccgaa gaaactcttg
    26161 ctgcatgcaa tagactgtgg tcttcaacaa accggtacct tttgtgttct tcatttcctc
    26221 atgtcgccta gttggttcct atcggaaggc gtgttcgcga atcgaatcac ttcttggcgt
    26281 gaagcacggt cctcaatctt cgttctcatg ccctaactgc tgaagttagt ttggtatcac
    26341 aagcaataga tttcatctgg tcatatggcc ctagctatca tcagtcctaa cgtcctttat
    26401 gttagattct tctcagttcc ctcgctccgc atagggttct cgattaggaa atcattcggt
    26461 gatttcatct tcgcccaccc tcaccgatcc gagctggttg atcatccatt tatttgtgat
    26521 gttacaccga ttggacaacg cgacgaaaga ccggctcttt gattccttca cggcaggcct
    26581 ccacaatctg ctgacacggc aagcctacta gcgaacgacg ctgcaattct ttggctggct
    26641 cgaccgcgag cagttcctcg tttgctgtct cgacgccaaa cacaccatca tcggctccct
    26701 gacattcagc atcgtccatc cccgcgaagt cttcaaatcg gctatcctcc tgaatacctc
    26761 cacgagcatc tgctcccacc atcacccctc gggagatccc gaacccagtt ctgaggatcg
    26821 tggctcacca cccgtctcta ccaggctggc gacctgctcg gcatcaccgt gctcgatcac
    26881 aatcttctgt gcgacgatcg tatctactgc tttgcagatc gcggctgact gacgtgaggc
    26941 ccaccctctc tgtcctgtct ggtagattgc tttgccattc ctggccgttg cttggccgcg
    27001 ctccggctcc cgagcgcacc gttcgcgcca ggaagacctg tcacgcccgc cgcttgttct
    27061 cgtccgcctg cgctgtttct gcaagcacgg ccccgtcacc tcccgttgtc ctctctggct
    27121 ctccgacttt agcgagagag ccagtgagtc caacaacaag gaggtgccga catggcaaag
    27181 caacccacga gacaggatat ctacacccgc gtcacgagcc agatttcgct ccaacttgaa
    27241 aaaggcgtgc gcccctggat caaaccgtgg agcgtcgaac atactgccgg gcgaatcacc
    27301 cgcccgcgcc gtcacaacgc cattccctac cgtggcgtca atgtcctgct gctctggtcc
    27361 gaggctctcg ctaagggcta cgctgccccg ctctggatga cctacaagca gtccgaagag
    27421 atgggtgcgc aagttcgcaa gggcgagcat ggttctctcg tcgtctttgc cgatcgcatc
    27481 accaagaccg aaaccgacag tgccggccag gatgtggagc gtgaaatccc cttcatgaag
    27541 gggtacaccg tcttcaacgt cgaacaaatc gacggccttc ccgcgcacta ctacgcgcaa
    27601 cccgttaatc ctctgcctct ctccgatcgt ctcacgcaag ctgaccagtt tgtcaccgcc
    27661 accggcgcgg ccatccatct tggcggcaat caggcttatt actcgcccgc caccgaccgc
    27721 atccagcttc cgcctttcga ggcgttcaag gacaaggaaa gctactatgc caccgcgctt
    27781 cacgaactca cgcactggac gcggcatgaa aaccgtctcg accgtgattt cggccgccag
    27841 cgtttcggcg acgcgggcta tgcccgcgag gagctggtcg cagaactcgg ctctgcattc
    27901 ctctgtgccg acctcggcat cactccggag atccgggaag accacgccga ctacatcggc
    27961 cactggctca aagtccttgc cgaagacaag cacgccatct ttcaggcggc cgcccatgcc
    28021 caacgcgccg ccgactacct gcagcagctc cagcctcctc cggtgcagca tgcatcgtga
    28081 cttccaaaga gcctctggct tcaccgccgg aggctctttt cctcctcccc gtcaaaccct
    28141 cccaaaagga ttcttgtatg tatgataaag gacgttatca tgcgaaagaa tgaaggccag
    28201 accctggctc gaagctcgaa catgcacagc gtgtcgccgg gcatgtcgac ccgaagacca
    28261 cccggtgata cgatcgtcgt accgacgcga tcaccctcga cgaaatcgaa cgcatcgaca
    28321 tctaatcccg ccgtccgtgc tgctgccctg catcctgctc ctatcgtgag cgacaacatt
    28381 cctcatcccc gcgtgtggtc ctgaccatgc cgccaagcct gagcctgggt tgcccgctca
    28441 tgtgttggat ggtgtagcca ggtccgccgc cgtcgtccgt gctttccccg ccgcgctgca
    28501 ccgtccccgc caactccgag ccgttgtcat ggcctgtgct ggtcgcgctc tgggccgcac
    28561 gggacgccgg aggctcccag cctccttttg tgtgtgtgtt gccctccacc tcccgcgaga
    28621 gggcgaacac ccacacgaat caaaggaggc tcacacatgg ctacgccatc caacacaccg
    28681 aaacccgttc aatcattcac atgcggcacc gtccaggccg tgatttggaa gaatacggga
    28741 aagaaaggag agttttattc ggtcacgctc ggccgatcgt acaaggatgc agaagaaaag
    28801 tggcatacct cggactcgtt cggggtgaac gacctctcag cggtcaatac cgtgctcgaa
    28861 cacgcggagg cgtggttgcg tgaccattcg aagtaatcat aaccgagagg tcctggcctt
    28921 ctggtctgga cctatgccct caccatccat cacatcattc tcgaggaggc ttgacgccat
    28981 gacagactat cagaatttcg cgacgacgat tgctactctc cttggcggcc gtcaatccgt
    29041 tcgtatcacc agcggaaatt acatggccct gtgtatcgaa tcaatcggcc attctgctga
    29101 aggtaatccc ttgatctcgc tcgcacatta cggggtccaa aatggcgact tgatggcgga
    29161 cccagaaatg acctttgaag tgtatgccac tccctcaccg gcagcggagc cgctgaccta
    29221 tcgcaacgat tatatgggca tgatgcaaga agtctatgag tacagcccta gcgggaagaa
    29281 atcacacatc cgaccacagc tcaagaagga tcttcgccac ttcgccgcgc tctggtttcg
    29341 caatctcaag acgcaaggct ttctctcaga gagcgccgtg cgggagatcc gctcgtgagg
    29401 cctcctgtct tcacctcccg cgtggcctca cgcgggaggt ttcccatcat cgcttcggtg
    29461 ttccagtgcg gtccagctcc cggaccccat gacgaccctt gcgcctgagc gagccgcctc
    29521 ctacaatgcc tccctaaaat cactttacga ggcgctccat gccgttcgtc cttcgcccct
    29581 accgtcgctt tccggtgcaa tgctccgtca cctacaatgc agggccattc caaggccatg
    29641 gcaccgtgtg gaatctctcg tgtgccggct ggcgtctctc tggcgatctg cccatgcgtc
    29701 caggggaaac catctcgctc accatcacac tccccaatga gcaacggatc gaggtgccgg
    29761 aagccgtggt ccggtggtcg agaggagaag agtttgcggt agagaatatc gccatcgagc
    29821 cacacacaca gatgcggttg cagcattatg tcaaacgact ggtgcaggaa ccagctggag
    29881 cggttgtgag agattgagta ttgagatgga acagagggcg gcaaagatga cgacgcacta
    29941 acaaggaggc tgcgatggga ttaaagttag tcagattggc actcggtata ggtctgggaa
    30001 ccattctgct gtttgccctg ccgttgttcc tctctaccgt gaccgactcc tcggaagttt
    30061 acgcagccga acctctcgat ctcaatacag cgaccgctga ccaactcaaa gccttaccag
    30121 ggatcggcga ggcgtactca gaaaagatca tcaaagcccg tccctacaag cggaaggatg
    30181 agttggtgca gaagaagatc attccgcaag cgacgtacga caagatcaag gatcagattg
    30241 tggcgaagca gagatagccg tgtaaggggt acaccatgcc tacaatggac gatgttcgta
    30301 gagagactca ggagtttata agaaggcact gggacataag taagttagac acggaagaaa
    30361 gtccgatatg gtgtgaatat ggccttactg ggacttgtcc gaatgaagac cggcctgggt
    30421 gctatgccct cgtagagggg ggggaagtaa cttatatagg gtcgggagca agccaaggac
    30481 acgggatata cgaaggatgc ggattaggcc ataggctcaa tggctacctc caatatgaca
    30541 ggactcaacc tgctgcaaca ctggcagatc gtcgctacaa acccaaaccc caatacactt
    30601 ttacatctct tcacactatc gggttcccac gcgtctatgg ttacatggct ctcgcgcttg
    30661 agtcattcct tctgaaccga gtgccgacat cggataataa acatcgaccc ggggggagtc
    30721 gaagtggaac tggaaaggct tcaggcaagg ttaaggttaa tggctaaagc cagatcatcg
    30781 gttattacat cctgaaatcc tgagcaagat ggtcctacct aaacgtcaac aactgcctcg
    30841 tttaggaatc ttaacactcg ctttcctcct cattgcgaat actgcattcg ccaacttctc
    30901 cggccccgtc gtctccgtcc tcgacggcga caccctcgaa gtcctgcaca accaacaccc
    30961 cgaacgtatc cgcctcagcg gtatcgactg tccggagaag aaacaggcct tcggccagcg
    31021 agcgaagcag gcggcctctg ccctcgcctt cgggaaggag gtcaccgtcc agacccacgg
    31081 ccacgacaag tacaggcgca ccatcgggga ggtgaccctg cccgatggca tgcatctcaa
    31141 tcaggagctg gtgaagcagg gctggtgctg gtggtatcgg aagtatgcgc caagagatac
    31201 ggtgctggag cagctagaga cagacgcccg agagggccgg aaaggattgt gggccgaccc
    31261 gcaaccggtg ccaccgtggg aatggcggaa gaaaagccgt tgagaccaac gtatttgtaa
    31321 caattcagac tcgacctggc agtcagtgtc agctatcgac
//
LOCUS       DAORRQ010000051        29567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_99_length_29567_cov_24.006370, whole genome shotgun sequence.
ACCESSION   DAORRQ010000051 DAORRQ010000000
VERSION     DAORRQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 29567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29567
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99_length_29567_cov_24.006370"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1281
                     /locus_tag="PK224_14960"
     CDS             94..1281
                     /locus_tag="PK224_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17094.1"
                     /translation="MTASRIVLFCLLFWAYIEPAMESMAAEWSLDPSIGVRGEYNTNL
                     LITHLDHEAVWGTWVSPGIKFAGTTERLEISGKASLDFVRYTGGQDEEFTNVFLPLSA
                     RYKTEHDTFGFDGGYTRDNTLMGELLTTGMLIRFTQRNLWTASPTWTRELTEQLSFQV
                     GYQFTNASYENGRRLGLLDYELHGGTAALIYQASERTKVQVLGNYLRFETKGVPLQAS
                     APGAALSLTHSFSESLTVTAAGGPRVITTSFQSSSVGLKDSETVWTYNASILKQFEHS
                     GIQIGYSRDIFPSGFGLLIRNDQVSAIVSTTLSHSLTAALDARYNWVTGQTTTALGGR
                     LPDATFIHLSPKLTWRINEWWSLESSYTYRTFDAETFSNAGHSHSTQLMLTWHPTKFT
                     ISR"
     gene            1300..3045
                     /locus_tag="PK224_14965"
     CDS             1300..3045
                     /locus_tag="PK224_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRB17095.1"
                     /translation="MQDSSPDFKELFDIARRRKKPILVTTIVIATLGAIVAFILPAVY
                     RSTAMILIEEQEIPQDLVRSAVTSYADQRIETIKQQVMSRATLWKIVEQYDLYHGMRE
                     VSPTEAVLERFVKDIQIEVINAKVVDKRTQNPTQATIAFTLAYDGVSPEVAQRVANEL
                     TSLFLGENMKTRERRAQETTAFFKQEADNLSRHIEDLERQLAKVKQKADGALPELTQL
                     NMQLLNGAERELTDVDRDIRSLEERKTSLQGELATIKPNTPIISASGERILDSGERLK
                     ALRAEYADKAAYLSSEHPDIAKLQQEIHALEKDTGGQAQVDDVVKRLAGERATLIALL
                     DRYGEAHPDVVRARRVISSLESELNQEQGKFLKLPFIKPENPAYINIHAQLSSTSATL
                     DALKDQRTKLKRRAEEYARRLENTHRFEPEYLDLARERDNSTRKYEELRSKLLEAQVS
                     EGLEVQHKGERFSLVDPPDLPSKPSKPNRLAILLLSFLVAAAGGVGSGIVAENFDRSI
                     HSPESLAKLTHAYPLAVIPYMPNNRDLQRLIAKQRTTRLASLGIAAVAILLVHFFWLP
                     LDVVWFAALRKFGLE"
     gene            3064..3900
                     /locus_tag="PK224_14970"
     CDS             3064..3900
                     /locus_tag="PK224_14970"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HRB17096.1"
                     /translation="MEPLHSAMERFKKDEQRRPVSAVPKSAPSRAGKAPVEEIVYTQT
                     RTIDIADEVLRERRVLAGFERGAFLDSYKILRTQVMHRLREHGWNVLGITSPGKQEGK
                     TLTAVNLAISLAMDTTQTVLLVDADLTNPSAHHAFGLEECGGLVDYLLDGIPVEHLLI
                     HPGIGRFVLMPGGRAIQHSAEALTSPKMASLVEEFKHRYPSRIVLFDLPPLLQTADVL
                     AFSPYTDALLLVVEDGKTSREDIERALQLVKGSTPVLGTVLNKAGKAQMTPATMKRML
                     LT"
     gene            4333..6483
                     /locus_tag="PK224_14975"
     CDS             4333..6483
                     /locus_tag="PK224_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HRB17097.1"
                     /translation="MKQILQHKRSGAVKVEEVPPPSLRGCGLLVLNEASLISPGTEKS
                     TIQSTKKSLVGKAMERPEKIKKVLSAIQTDGLALTLSRVFDKLDSPASLGYSCAGTVL
                     EAARDAGSFTVGDRVACAGQNYASHAEMIYVPKHLCVKIPDGVDSEDASFVTLGAIAL
                     QGVRQAEPRLGDRIAVIGLGLLGQLTVQLLKASGCRVLGSDVDQSKLLLASESGADMV
                     ALSSDLADAAAPFSDGHGVDAVIVTASTKDNGPIEAAGQIARKKGKVVVVGAVGMNVP
                     REPYYQKELDLRLSMSYGPGRYDTAYEEHGHDYPFGYVRWTEQRNMQAFLELVATGKV
                     RLKPLITHRFPIEQAESAYALMMEGSTPYIAMVITYPSDRARPLPRTIAVGTAPAARP
                     VMLGIIGAGNHVKDMLLPPLQNMENVGIRGICTASGITAKTLAGKITAAYCTSDSQSI
                     LDDPAINTVLIGTRHDSHAALTIKALLAGKHVFVEKPLCLTEDELKDIRSVYEKKAPD
                     GLQLMVGFNRRFSPHAQEAGRFFASRVNPLVMLYRINAGRIPADHWVQHPDIGGGRLI
                     GEMCHFVDYMQALSASPPTSVYASRIGRHSSGITDDQCSIVVNFADGSIGTIIYTAEG
                     NSELPKERFEAHVDGKSLVMNDFIETQTYKDGKSTVFKTAKRDKGFTEEMSRFIKSVA
                     GGQAPVIPFEQIDAVTRACLLAARSLISGAVYLL"
     gene            6643..8067
                     /locus_tag="PK224_14980"
     CDS             6643..8067
                     /locus_tag="PK224_14980"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB17098.1"
                     /translation="MYERFYNLTLKPFTLVPNPDFLYRGETHRLALTTLEYGILNQAG
                     FVVLTGEPGMGKTTLIQQILAEHRNHFTTGLITNTHAGMETLLPWILLAFGQNEKSLD
                     KLEGFHALQQFLDKEASAKRRVLLIVDEAQNLGANLLEELRLLSNLNKDKAPILQIIL
                     SGQPDLRQLLQRKDLTQFAQRVMVDYSLEPFKEEDSLGYIAHRLRVAGREEPIFTHHA
                     TRMIHRLTGGIPRLINQLCDTALTYGFAEEAPFLSTKIVAQAAKDRSKGGILPLTDTD
                     ALTAMTPEQDAAEQAQLATFASRTASTPSLGDRSIKTVEATTAARAPSHDAGEAYERG
                     LALKKVGLHKDALQQFSLAAQDAALTFRAMAQVGICLKSLGQSEDAATAFRKALQAQR
                     GTSVDTIQVRYLLARTLESLGRIDDTLEHYRWIRREEPNFKDVAERIERLGGRRSPSP
                     RNAEGTGDGSSWVAQLQRILGASK"
     gene            8157..8879
                     /locus_tag="PK224_14985"
     CDS             8157..8879
                     /locus_tag="PK224_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17099.1"
                     /translation="MGLEIIEIVEDDQGQAKLLDQILRQASFRTNVAFDGPSAMQDVW
                     RIKPSLIMADDNLPGLSGREICKRLRQDPSTKYIPIILMSGYSSEERRVEALDGGADD
                     FIVKPYAAGELVARVRALLRRSQQGQGQEEELGEDLALTESLYVAVYRGQKLTLSVHE
                     WKSLRRLTNTVGSVVPREELSTLLWGDDALMHDGEIDRCMEQLNKKLAGEGPAAGHIK
                     MVPGGFRLIIPPSEKPDIVQAQ"
     gene            complement(8847..9710)
                     /locus_tag="PK224_14990"
     CDS             complement(8847..9710)
                     /locus_tag="PK224_14990"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRB17100.1"
                     /translation="MRKSRSPIHTAPPGFGQDVVQAAAAYQATVLRLMRHAMAGDGTA
                     DTIHSIRTHCRRLQALLELCNNRDRAAVMARTVSRLSRLRALQVFRQYLMKIEAPESD
                     ITAVGAWIVERERKLTRAQTYRKIEQAVWKQALPMITPPNLSVKHRLEVMRHEHERVL
                     SRLIEKAVEKPRRKRLHALRLALKTIRYQTEWLPGQGATRQDGLKRIKQVQALLGRYE
                     EMADFRRWGKKLNLTVQARIERDWKRARRRARRVPEELRWLLDALAAGRLWSEGDRKG
                     ALLSLDDVRLL"
     gene            9830..10327
                     /locus_tag="PK224_14995"
     CDS             9830..10327
                     /locus_tag="PK224_14995"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HRB17101.1"
                     /translation="MDCLFFRHGIAIERQEWSGREQDRPLTDKGVARTRESGKGLLNL
                     RIKPTHIFSSPLARAHDTAAILQSLIRSNPKIRICGELNPTAAPRTLFVLLDALPPDA
                     VALCIGHEPHLSLAAGILLTGKPCSALSLKKAGACLIHIEESVRPAAGRLEWWLTAAQ
                     LRALA"
     gene            complement(10600..12153)
                     /locus_tag="PK224_15000"
     CDS             complement(10600..12153)
                     /locus_tag="PK224_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRB17102.1"
                     /translation="MPKLAVLDIGTNSIHMVLAEVQPDYTYKILDRFKDMTRLGDGVF
                     TSRRLSDQAMMRGLEVIRNLVTLARNKGYERIEGVATSAVREARNGGEFLDHVAQQTG
                     LTVRVITGAEEARLIFLGVQNSVALPEPPTLVIDIGGGSVEVIVGNRETIYQARSLKL
                     GAIRLKDLYLTKTPPSKAMLHELEEAVTGQLKQALGTYKTKRVEQIIATSGMAGNLAE
                     VIYLQRTGRPLQQLNLTKISAKEIAAVEKRLADATLKTRLAMPGLDPKRVDTLLPATM
                     VFRILLDLLRKDELTICDKAIREGIIYDFIQRHHEGIQAERDIPDVRKRNILALARRC
                     HVSETHALHVAGLALRLFDQTTSLHGFGQREREWLEFAAILHDIGYLINSRQHHKHAY
                     YLIKNSDLSGFTAEEIDLVANIARYHRRSVPTRKHGEFTALPEGNQRIINVLSALLRI
                     ADGLDRSQFSVVRNVDVKIGKTVMITVDVSGDAELEIWAARGRSDLFEKVFKRPVQFV
                     TTSQEDEVT"
     gene            complement(12154..12822)
                     /gene="phoU"
                     /locus_tag="PK224_15005"
     CDS             complement(12154..12822)
                     /gene="phoU"
                     /locus_tag="PK224_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRB17103.1"
                     /translation="MQRHFDQDLAHLKQTLLRMGGLVESQIQLALQALVDRDSDLAVD
                     VIKQDHDVNALDVEIDELCIQLLALQQPTARDLRFVTTGMKISSELERMGDLADNIAQ
                     RALELNGEPQLKPYIDIPRMANWTMRMVKDCLDAFVNSDPVLARKVCADDDFVDEVNE
                     QLFRELLSFMLEDTRTITRAIRLTFVAKSLERIADHATNIAELVVYMVEGKNIRHVAP
                     SASM"
     gene            complement(13059..13922)
                     /gene="pstB"
                     /locus_tag="PK224_15010"
     CDS             complement(13059..13922)
                     /gene="pstB"
                     /locus_tag="PK224_15010"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRB17104.1"
                     /translation="MDIQTRSLSPTAPALSLRPTVPQPRLTRPHEVHGTSVDAKVLVR
                     NLDFYYGQRQALFRVSLTVRTHSVTSFIGPSGCGKSTLLRCLNRMNDLVEGARAVGRV
                     ELDGVDIYDPAIDVTDLRKRVGMVFQKSNPFPKSIYDNVAYGPRLHGTKDKRSLDELV
                     QHSLQGAGLWDEVKDRLMQSALGLSGGQQQRLCIARALAVQPEVILMDEPCSALDPIA
                     TGKIEELIYTLKDTYTVVIVTHNMQQAARVSDQCGFFLMGELVEFGDTKTIFTTPHDK
                     RTEDYITGRFG"
     gene            complement(13941..15590)
                     /gene="pstA"
                     /locus_tag="PK224_15015"
     CDS             complement(13941..15590)
                     /gene="pstA"
                     /locus_tag="PK224_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRB17105.1"
                     /translation="MGERSTLMKDFWRGGELFVWMTASGVALSLLMVAGMLGLIMING
                     LGQFWPGALQETTLKTQEAVIGQVVGEEVIPNSITPDLPAGQRRFRYRVGNRDVFGSE
                     YRWINAADIVSSAHPEDLTLVERREWGPAFGRITVGVEGAATGSASGSSWTTVKTLVE
                     RANQLRRRIEHLEREEIGSINYRIEKARLARHALTLGGRLAPGDAEKDQRLLDQITAL
                     ERDYLTKTEALERLHKQAGQEVLFLTVSTGRSVRLSLDQVLAVSRPNGMHWGEKLLAY
                     VQSLWLFVSTEPREANTEGGIFPAIFGTVLMVFLMTLAVMPFGVMAAVYLREYANQGL
                     LVRLVRIAVNNLAGVPSIVFGVFGLAFFVYALGGTIDQWLFPEALPNPTFGTGGILWA
                     SLTLALLTVPVVIVATEEGLSAVPRDFREGSLGLGATKFETLRHVILPCALPGILTGL
                     ILAMARAAGEVAPLMLTGVVKLAPALPLDEYLPFLHLDRKFMHLGFHIYDVGFQSPNV
                     EAAKPMVYMTTLILILVVVLLNWAAVRLRNRLRRRFTASAV"
     gene            complement(15583..17787)
                     /locus_tag="PK224_15020"
     CDS             complement(15583..17787)
                     /locus_tag="PK224_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRB17106.1"
                     /translation="MLRPVHLRTLFDRLARAIITVGGLATIVSILGIFFFLFREVTPL
                     FTAPSAKLTQRLSVPALLVDEGPAQVAVDEHREIAQVFTSGAIQFFDLASGQPIPLEM
                     PAQVTSRQITAMASGGGNAPRLAVGTADGEVLFLKVGTTTEFTDQGERRKRPHIRAGQ
                     PIPLTKGPIVRLAYRANDHGSLLALLSKGGRLLVVRIAADDVAGDHPIITELPQPKGA
                     VTALALDALLENLYVGTADGQVVHVALSETESPEMRAAYTVAASSTAVTTLGFLSGDR
                     SLVVMSEDGAVSTWGLVRRADAPSGWTLIQIHTLRAHNAPVTAFAPSQRVKGFVTGDM
                     KGNLFLHHATSSQTLLRLANSEFPIRAVAFAPKGDGLIALDGAGQLSLYQVQNAYPEV
                     TLETLFGKVWYEGYDQPAHVWQSSSGSDDVEPKLGLLPLAFGTLKGTMYALFLAVPIA
                     ILAAMCTSQFMHQDLRAKIKPIIEVMAALPTVVLGFLAGLWLAPLLERMLPAVAGMAL
                     MLPLLVVVASFAWYLCPLSVKRHLPQGQEALLLIPILGLGIAGCLWANPLIEGWWFDG
                     NVKAWLSSRLGIQYDQRNALVVGLVMGFAIVPVIYSIAEEALSNVPKIQIAGSLALGA
                     TRWQTVLHLVLLSASPGIFSAVMIGFGRAIGETMIVLMATGNTPILDWNWANGFRTLS
                     ANIAVEIPEAPHGGSLYRVLFLAALLLFIVTFVINSLAELIRQRLRDKYSHG"
     gene            complement(17811..18830)
                     /locus_tag="PK224_15025"
     CDS             complement(17811..18830)
                     /locus_tag="PK224_15025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRB17107.1"
                     /translation="MRRESSIVRESESWHPLTTVLVFSLLYALIIMPAACAEQSDGAE
                     TTLRPYQKVSGVSGTINSIGSDTLNNLITLWAEGFRKQYPNVKIQVEGKGSSTAPPAL
                     IENTAQLGPMSRTMKSSEIDAFETKFGYPPTAYPVAVDALALFVNKDNPIKGLTVAEA
                     DALFSKSRRQGHAVELSKWGQLGLTGDWAAAPVSLYGRNSASGTYGFFKEHALKNGDF
                     KDQVKEQPGSASVVQGISEDRYGIGYSGIGYTTSGVKMLPLAAKAGQPFIEPTRANTR
                     NGTYPLWRYLYIYVNQAPGKPLSPIVKEFLAYVYSREGQSDVLRDGYFPVDATLAEKQ
                     LSQIR"
     gene            complement(19088..19792)
                     /locus_tag="PK224_15030"
     CDS             complement(19088..19792)
                     /locus_tag="PK224_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17108.1"
                     /translation="MSEREPPDSSFALATVCKSDLVRLGLQTVLTTQPHLRLVGEAIN
                     ASDAEEIVAREKPRALIVVLESEIDVAELVRRVRISAPTIRIIAVSDMEYRHGMSGLL
                     LSGIDSIVLNIQPVEILLATVDYLRRLPGTSRRQERNDPTPSDSREMTVCQDPASPSL
                     HERLDSPLTDREQEIALLVGQGLSNKDIADLLHISTITVRHHLTNLFQKLGISTRLKL
                     LRWVHQKDLAGCRPAV"
     gene            complement(19836..20147)
                     /locus_tag="PK224_15035"
     CDS             complement(19836..20147)
                     /locus_tag="PK224_15035"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB17109.1"
                     /translation="MAQRRIKSSADNKQTGSPPKSVKKTSARPRPVRDRRPRAAGGLA
                     GAEEIRTRIEKLAYELYQRRGRQDGHDRQDWLEAERLTLSQPAATRQDALDRLSGSSY
                     V"
     gene            complement(20364..20534)
                     /locus_tag="PK224_15040"
     CDS             complement(20364..20534)
                     /locus_tag="PK224_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17110.1"
                     /translation="MFSWSNSRTPLPSEQGSANLTLTVLCPFMTIHLVSAGSGASSDL
                     AQDNESARADPV"
     gene            20752..21456
                     /locus_tag="PK224_15045"
     CDS             20752..21456
                     /locus_tag="PK224_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17111.1"
                     /translation="MGSRIVQIIEDEPLHADLLDRALRQAQFATTLAADGESGWRDAQ
                     RLLPSLILLDLMLPGLSGHDICRLVRRTPSTRHIPIIMLTAVGTEADRIAGLEMGADD
                     YVVKPFSPREVVSRVQAVLRRVHETRREDPVLFSDTSITMEGPYFVLSLQERQITVSN
                     TELRLLQYLLPRNGELVRGDELLNLPGEELGSTSREELEHRVRFLRRKLENSGTGSIE
                     ILPGSRYRFLAHPRPT"
     gene            complement(21565..21804)
                     /locus_tag="PK224_15050"
     CDS             complement(21565..21804)
                     /locus_tag="PK224_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17112.1"
                     /translation="MVHPSISPTSEGLILDLVCLRKSLTLEQVVTLLPELSWNQVFKT
                     VDELSRRGEIILLRRGFEYEIERISSRARVLACVG"
     gene            complement(21967..22476)
                     /locus_tag="PK224_15055"
     CDS             complement(21967..22476)
                     /locus_tag="PK224_15055"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HRB17113.1"
                     /translation="MIVTNLEPRAHRFSDFLGRAIDTDLTELWMKGIKAVLSLLILTI
                     LIALTGGVVKTFLDIGLLLHAPVEIGLRQIIVDTLILLAVVEVFKTTLTYFSEGRVKV
                     TFIVDTILVVMLTEIISLWFKDADHMKLLSLGAILLALGAIRVVAVRCSPAQGDGTGR
                     GSFCGREEI"
     gene            22669..22854
                     /locus_tag="PK224_15060"
     CDS             22669..22854
                     /locus_tag="PK224_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17114.1"
                     /translation="MVRIKVLLVINYIEVLFREVHCLVTALLLPAKQNVAAYRSRIEG
                     RLRIVLDSHPEHEGRKS"
     gene            22992..24155
                     /locus_tag="PK224_15065"
     CDS             22992..24155
                     /locus_tag="PK224_15065"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRB17115.1"
                     /translation="MTRQEQTGSRHHLGTRSILTAVATAAVLCLFAGYAGAEHAGLSG
                     TGGPAVDPAIEPYTNHNGLHGKLSIAGSDTMRPLISKLSAQFLSLHPGLQIAVEGTGS
                     STAIREFLLGLSYQRRGDKVQSKGTAGSSTVDLLASSRQLTEDESKAFVSNYGYRPLE
                     VPIAMDAVAVYVNKDNPLQRLTLAEVDAIFGKDRKRGLESITNWSQIGLGNSALAKQP
                     VHLYGRDKRSGTREFFKHVALKDGELLDDVIEQPGSASEIIAIAQDPLGVGYAGAGFV
                     ISAVRQVPIAAQSEGPALLPSVETVTSGAYPLGRSLYLYVKKSPTDKLDPVVEEFLAF
                     VNSRQGQETVARANFYPLTSTQVAKNRQDLGLAKGAMVILPSPDLQVAEQPTR"
     gene            24321..25016
                     /locus_tag="PK224_15070"
     CDS             24321..25016
                     /locus_tag="PK224_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17116.1"
                     /translation="MKPTLRRTVIISVLLAATAHFGCTTPPPPRTSYQDPITAIRLYV
                     DDRARSGHQHPADVSSEQIAKVLGGLRVIPRSGFIGSLITGQAQATPAFSSTEIQALA
                     PRLSRALTEAKPDELVTFYRRFSDSSTGLAITSGGMFVQDGYLVVVLANNRTLPTDGM
                     NQNMVTDFDPVDSPLVPISRTSFRVEFAHPSALAAPDKRPSWPYIDEGRILAIDLRRL
                     STESKSPSVPPAP"
     gene            complement(25384..26895)
                     /locus_tag="PK224_15075"
     CDS             complement(25384..26895)
                     /locus_tag="PK224_15075"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRB17117.1"
                     /translation="MKYGRIHVLKRSAATLGLCGALFYLPLPGSAGANDAAPTVVAQG
                     PAQSIQALSAMETVPQVTPTANGTDRQTSGQRLSIESTSLESLLLEKGVITQEDWIRL
                     KAEEERRVFEQSAELQMAGNPRWYERIRITGYIQYKYNMAATNRLFDNPLGDSFGDQQ
                     GNEFYIRRMRLVFQGQVSERVAFFTQFALEGGQQTPANNEMIDAYADYYLTKDKVHRL
                     RFGLQRVPNSFDTYRSSTNRQEMDRHESIQSGAPGERDLGVAYYWSPKIAQERFAQLA
                     AYHNGPGDYGVFGVMVYNGQGRSRFELNKDKHIGAKVSYPFQFPNGRLLEAGVLGYHG
                     VFSVQGASQPGGAGAARCISPLTSEGGCDIKDQRVTAYVWTPPQPWGLLAEFTVGRGP
                     KRNAQTNFIEESNVVGGYVQGYYTWRYSDVGMLTPYVRYGEYYGGFKSLTGAPDGQSR
                     TWNLGLVWEPDTHFRVVADWMFKDGLNSTLVANRAQSDFDASQLRLMTQWFWN"
     gene            27151..27846
                     /locus_tag="PK224_15080"
     CDS             27151..27846
                     /locus_tag="PK224_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17118.1"
                     /translation="MITSGTKTPLLLVSADKVSSRSLAQLLETNGYSLSVASTLTSAQ
                     GEIRRLSPALILVDRDILAQETGRRDQFSPTIPIIVLQPFDKSCGPDECLTELEAGYD
                     LVFCSPHYRELLAHIRAILRRHKMISASPAVLRVQGLAMDTARHEVTVGGALVALTPK
                     EFRILHQFLLSPGVVLSRQDLLNRVWGEDYALEEHALDVHIHSLRQKIEADPSKPIFI
                     VTIRGIGYKLQTT"
     gene            complement(28083..28466)
                     /locus_tag="PK224_15085"
     CDS             complement(28083..28466)
                     /locus_tag="PK224_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB17119.1"
                     /translation="MVTVSDLMTKKLVTVPAGISESEAALVMNDLDVGSVFIEQNDRV
                     VGIVTESDIVRKVVGENRPVHFVPVESIMSSPVISLDERRSITEAADLMQHHHTRHLG
                     VLKSGAIVGVLSVRDLLQPVSVDEF"
     gene            complement(28679..29254)
                     /locus_tag="PK224_15090"
     CDS             complement(28679..29254)
                     /locus_tag="PK224_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB17120.1"
                     /translation="MIRLRPFVLLGLLAVAGPLFLDGCASKSGTSGGVTATPPKPRAE
                     ERVAAAPVQEIPAPPEPAPVPLRSVEMAARNATGEQNIPFPDVLFDFDQYVLRDDALT
                     AVEANAKRLKDNRITKVLLEGRCDEVGTSEYNLVLGERRALSVKRYLESLGMSQLQVD
                     TTSYGKDRPLCLQHNPVCWQKNRSVHFVVKE"
     gene            complement(29329..>29567)
                     /locus_tag="PK224_15095"
     CDS             complement(29329..>29567)
                     /locus_tag="PK224_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17121.1"
                     /translation="LRRAREDLHRTIDPLLPEERRKASLSQKALWILASTPGVTSVLN
                     GMRTPAYVDDALQILRWEPLSDSRRVYDCCAAKK"
BASE COUNT         6448 a         8786 c         8312 g         6021 t
ORIGIN      
        1 cacgtcctcc ataggcgtca tgatactacc attttaaccg ttacagtcca ctagtttccg
       61 taagtcatcg cataccgcat gaatcaagga cccatgacag cttctcgtat tgtccttttc
      121 tgtctgctat tttgggccta catcgaaccc gcgatggaga gcatggccgc agaatggagc
      181 cttgatccct cgatcggagt ccgcggagaa tacaacacca atcttttgat cactcacctt
      241 gatcacgaag ccgtgtgggg cacctgggta tcacccggca tcaagttcgc gggaacgacg
      301 gagcgattag aaataagcgg aaaagcgagc ttagatttcg tccggtatac gggtggtcag
      361 gacgaggaat ttacgaatgt cttcttgccg ctatcagcgc ggtataagac cgaacacgat
      421 accttcggct ttgacggtgg gtatacacgt gacaatacat taatgggaga attgttgact
      481 accggaatgc ttatccgctt cacccaacga aatctctgga ccgcaagtcc gacctggacc
      541 agggaattga ctgaacagct ctcgttccaa gtcggatacc aatttacgaa tgcctcctac
      601 gagaatggtc gacgcctggg ccttttagat tacgagctac atggcgggac agctgcctta
      661 atctaccagg cgagtgagcg aacaaaggtg caagtgctcg ggaattatct caggtttgaa
      721 acgaagggag tccccctcca ggcatctgct cccggagcag ctctgagcct gacccactcg
      781 ttttctgaat ccctcaccgt cacggcagcg ggaggcccta gggttatcac gacttcgttt
      841 caatcgtcga gtgtgggctt gaaagattcc gaaaccgtgt ggacgtacaa tgcttccatt
      901 ctcaaacaat ttgaacactc ggggatccaa attggctatt cccgagacat atttcccagt
      961 ggattcggtc ttctcatacg aaacgatcaa gtcagcgcca tcgtctcaac aacgttgagt
     1021 cactcgctga ctgccgccct ggatgcgcgt tacaactggg tcaccgggca gaccactacc
     1081 gctctcggag gcagattgcc agatgccacg tttatccatc tcagcccgaa gctgacgtgg
     1141 cggatcaatg agtggtggtc acttgaaagc tcctatacct atagaacctt tgatgcagag
     1201 accttttcaa atgcgggaca ctctcactct acccaattga tgctcacctg gcacccgaca
     1261 aagttcacga tatctcgata gagaatggag cataggagca tgcaggattc atcgccagac
     1321 tttaaagaac tttttgatat tgcccgacgc cgaaagaagc cgattcttgt aacgactatc
     1381 gtgattgcca cccttggggc gatcgtcgcc tttattttgc cggccgtgta tcgatctact
     1441 gcgatgatct tgattgaaga acaggagatc cctcaggatt tggttcgatc ggcagtcact
     1501 tcctatgcag accaacggat tgagacgatc aagcagcaag tcatgagtcg cgctactctc
     1561 tggaaaatcg tcgaacagta tgatttgtac catggcatgc gggaagtcag cccgacagag
     1621 gcagtgctcg agcggtttgt gaaggacatt caaatcgagg tcatcaacgc gaaagtggtg
     1681 gataagcgca ctcagaatcc cactcaggcc acgattgcat tcacactcgc gtatgacggt
     1741 gtaagtccgg aggtcgcaca gcgtgtcgcg aatgaattga ccagcctgtt cctgggtgaa
     1801 aatatgaaaa cccgtgaacg ccgggcacag gagaccacgg catttttcaa gcaggaagcc
     1861 gacaacctgt cccggcacat cgaagatctt gagcgacaac tggccaaagt gaaacagaaa
     1921 gcggacgggg ccttacccga actcacccaa ctgaacatgc agctcttgaa cggggctgag
     1981 cgagaactca cggacgtgga ccgtgacatc cgcagcctcg aagagcgcaa aacctctctg
     2041 cagggggaac tggccacgat caaaccgaat accccgatca tttcggcttc cggcgagaga
     2101 atcctcgatt ccggagaacg attaaaagcg cttcgagccg aatacgcgga caaggcagcc
     2161 tatctatcct ctgaacaccc ggacattgcc aagctgcagc aggaaatcca tgcgctggag
     2221 aaagacactg gaggccaagc ccaagtggat gatgtcgtga aacggctcgc cggggaacgg
     2281 gcgaccctga tcgctctgct cgatcgatac ggagaggctc atccggacgt ggtccgggca
     2341 cgtcgtgtga tcagttcact tgagagcgaa ctgaaccagg aacagggtaa gtttctcaaa
     2401 ctacctttca tcaagccgga gaacccagcc tacatcaata ttcatgcaca actctcctcg
     2461 acgtctgcca cgttggatgc gttgaaagat cagcggacga agctgaaacg acgagcggaa
     2521 gagtatgcgc gccgcttgga gaatacacat cgcttcgaac cggagtatct cgacctagct
     2581 cgcgagcggg acaattcgac ccgtaagtat gaagagttac gttcaaagct gctggaggca
     2641 caggtttcag agggcttgga ggttcagcac aagggagaac gattttctct cgtggatcct
     2701 ccggaccttc cttcaaaacc gtctaagcca aaccggttgg ccatccttct gctcagcttt
     2761 ctcgtcgcgg ctgcgggcgg ggtcggaagc ggaatagtgg ccgagaattt cgatcgctca
     2821 atccacagcc cggaatctct ggcgaagttg acccatgcct atccgcttgc cgtcattccc
     2881 tacatgccca ataacaggga tcttcagcga ctgatcgcca aacagaggac cacgcgcctt
     2941 gccagcctcg gtatcgccgc ggtagccatt ctgctggtac attttttctg gttgccgctt
     3001 gacgtggtct ggtttgccgc actccgcaaa ttcgggctcg aataactgag caggagattt
     3061 catatggaac cgctacacag tgccatggaa cgattcaaga aagatgagca gcggcggccg
     3121 gtctcggccg tgcccaagtc cgcaccgtca cgggcaggga aagcaccggt ggaggagatc
     3181 gtctataccc agacacgcac gattgatatc gcagatgaag tcctgcgtga acggcgagtg
     3241 ctcgcgggct ttgagcgagg cgcgtttttg gactcgtaca agattcttcg cacgcaagtg
     3301 atgcatcgct tacgggaaca cggctggaac gtgctcggga tcaccagccc aggcaaacag
     3361 gaaggcaaga ctctgaccgc cgtcaatcta gcgatcagtc tggccatgga cacgacacaa
     3421 acggtgcttc tggtggatgc cgatctgaca aatcccagcg cccatcatgc attcggcctt
     3481 gaggagtgtg gcggtttggt ggactattta ctggacggaa tccccgtcga acacctcctg
     3541 atccatcccg gcatcgggcg ctttgtcctc atgcccggcg ggcgggccat tcaacattcg
     3601 gctgaggcgt tgacctcgcc gaaaatggcc tccctagtgg aagaatttaa acatcgctac
     3661 ccatcacgaa tcgtactttt cgacttgccc ccgctcctcc agactgcaga tgtgctggcc
     3721 ttctcaccgt acacagatgc actgctcctg gtcgtcgagg atggaaaaac ctcacgcgag
     3781 gacatagagc gagcgctcca gttagtcaaa gggagcactc cggtattggg gacggtcctt
     3841 aataaagccg gtaaggcgca gatgacaccg gcgaccatga aacgaatgtt attgacgtga
     3901 ccatctcaga tacgtaaata agattcacgt gcaaaccttt ccatcgcctt tggtcgatgc
     3961 cgtttacacc attccggaaa cgcgcagctt gccgaagtag gccaaatgtt ttctcagcgg
     4021 ggactcggga tccggacaat cgcaggacgt tatcgccgcc gtcgttcagt tggagcagtc
     4081 cgcgcgaaac atggcctaca catctcggat ttgctcagat cggacgctgg aatcgggcac
     4141 tcggcgagct tcccaccgtc ccggcattta gtgatacggc ggttcgacgg tcccgcctgg
     4201 aaggcaatca gcttcaatgc agagttgcgg cgcgtcaacc cctggggcgt cggaggggcc
     4261 tggaaacacg tccggcagta ttgaagggac acagttgatt gcaggacaat acgggcgcag
     4321 gcagtctctt ccatgaaaca aattcttcaa cataaacggt ccggagcggt gaaggtagag
     4381 gaggtccctc cgccttcact gcgaggttgc gggctgttag tgctcaatga agcctccctc
     4441 atcagcccgg gaaccgagaa gtcgacgatt cagagcacga aaaagtcgct ggtcggtaag
     4501 gccatggaac gcccggagaa gatcaagaag gtcctctccg cgattcagac agacggactc
     4561 gcgctcacgc tctcccgcgt attcgataaa ctggactcgc cggcctcgtt gggctacagc
     4621 tgtgcgggca cggtgctgga ggctgctcgc gacgcgggat cctttacggt cggtgaccgc
     4681 gttgcctgtg ccgggcaaaa ctatgcgtcc catgccgaaa tgatctacgt accgaaacat
     4741 ctctgcgtga aaattccgga cggcgtggat tccgaggatg cgtcttttgt gacgctcgga
     4801 gccatcgctc tgcagggcgt tcgtcaggcg gagcccaggc tgggagaccg gatcgccgtg
     4861 atcggccttg gactgctcgg tcagctgacc gtgcaattgt tgaaggcctc cggttgccgt
     4921 gtgctgggat ccgatgtcga tcaatccaag ttgctgttgg cgagtgagtc gggagcggat
     4981 atggtagcgc tgtcctccga tcttgcggat gcagccgccc ccttttcaga cggccacggc
     5041 gtcgatgcgg tgatcgtcac ggccagcacc aaggataacg gccccattga ggccgccggc
     5101 cagatcgcga ggaaaaaggg gaaggtggtg gtggtcggcg cggtgggaat gaatgtgccc
     5161 cgtgaaccgt actatcagaa ggagttggac ctgcgcctct ccatgtccta cggccccgga
     5221 cgctacgaca cggcctacga agaacatggg catgattacc cgttcggata cgtacgctgg
     5281 acagaacagc gtaacatgca ggcgtttctc gaactcgttg cgacagggaa ggtgcgcctc
     5341 aagccgctga tcacccatcg ctttcccatc gagcaggcgg agtcggccta tgcgctgatg
     5401 atggagggct cgacacccta tatcgcaatg gttatcacat atccctccga tcgggctcgt
     5461 cctttaccgc gaaccattgc cgtcgggaca gccccggccg ctcgcccggt catgctggga
     5521 atcatcggag ctggaaacca cgtcaaggat atgctgctcc cgccgcttca aaacatggaa
     5581 aacgtcggca ttcgcggcat ctgtaccgcc tcgggaatca cagcgaagac cctggccgga
     5641 aaaatcactg ccgcctattg cacgagcgac agccagtcca ttctggatga cccggccatc
     5701 aacacggtgc tgatcggaac ccgccatgac agccatgccg ccctgacgat caaagcacta
     5761 ctcgcgggca agcatgtgtt cgtagagaaa ccgctctgcc tgacggaaga cgaactgaag
     5821 gacatccgct ccgtctatga gaagaaggca ccagacgggt tacaattgat ggtgggcttc
     5881 aacaggcggt tttctccaca tgcacaggaa gccgggaggt ttttcgcatc gcgagtaaat
     5941 ccgctcgtca tgctgtatcg aatcaacgca ggacgcattc cagccgatca ttgggtgcaa
     6001 catccggaca taggaggcgg acgcctcatc ggcgaaatgt gtcatttcgt cgattacatg
     6061 caagcgctct cggcctctcc acccacttcg gtgtatgcgt ccagaatcgg acgtcatagc
     6121 tccggcatca ccgatgacca atgcagtatc gtggtgaatt ttgcagacgg atccatcggc
     6181 accatcatct atacggcaga ggggaacagc gaacttccga aagaacgctt tgaggcccac
     6241 gtggacggca agtcgctcgt catgaacgac ttcatagaaa cgcagaccta taaagacggc
     6301 aagagtaccg tgttcaagac cgcaaaacgg gacaaggggt ttacagaaga aatgtcgcgg
     6361 tttataaaat ccgttgcggg aggacaagcc ccggtcatcc cgtttgagca gatcgacgca
     6421 gtgacacggg cctgccttct ggcagcacga agcctgatca gcggcgctgt ctacctcctc
     6481 tgacaagggc acaatcagtc ggctttccac tccaatgcgt tggcccacta cgggctattg
     6541 ttgcaagact ctgctcggcc tcggcgcctg actcaagctg gaacaaaatt ctgaagacga
     6601 atgtagttga cctctccttc cttatcccgt aagtaggaag ttatgtacga acgcttttac
     6661 aatctgacgc tcaaaccgtt taccctggtt ccgaaccccg atttcctcta tcgaggcgag
     6721 acccatcgtc tcgcgctgac gaccctcgaa tacgggatcc ttaaccaagc cgggttcgtc
     6781 gtactcaccg gagaacccgg gatgggcaag accacgctca tccagcagat ccttgccgag
     6841 catcggaacc atttcacgac cggactgatc accaacaccc atgcaggaat ggaaacactc
     6901 ctgccatgga tcctgctggc cttcggtcag aatgagaaga gtctggataa gctggaaggg
     6961 tttcacgcct tgcagcagtt tctggacaag gaagcttctg ccaagcgacg cgtgttgttg
     7021 atcgtcgatg aagcacagaa tctcggcgcg aatctcttgg aggaactacg cctgctctcc
     7081 aatctcaata aagacaaggc gcccatcctc cagatcattc tctccggaca gccggattta
     7141 cggcagctcc tccaacgcaa ggacctgaca cagttcgccc agcgcgtgat ggtggactac
     7201 agcctcgaac ccttcaaaga agaagacagt ctgggttaca ttgcccaccg cctgcgagtc
     7261 gcgggtcgcg aggaaccgat cttcacgcac catgccactc gtatgattca tcggctgacc
     7321 ggcggcatcc cacgcctaat caaccaattg tgcgataccg cgctgaccta tgggttcgca
     7381 gaggaggccc ccttcctttc aaccaagatc gtggcgcagg cggcgaaaga ccggagcaaa
     7441 ggaggcattc tcccgctcac ggacaccgat gctctcacgg cgatgactcc ggaacaggat
     7501 gcggccgaac aggctcagct cgccaccttc gcatcccgaa ccgcttccac tccaagcctc
     7561 ggcgaccgat caatcaagac cgtggaggct accacagcag cccgtgcgcc aagccatgac
     7621 gcgggggaag cctacgaacg cggcctggcg ctcaagaaag tcgggttgca taaagatgcg
     7681 ctgcaacagt tttccctcgc cgctcaggat gcggctctga cattcagggc catggcgcaa
     7741 gtcggcattt gcctgaaatc cctcggccag tccgaggacg ccgccaccgc atttcgaaaa
     7801 gccctgcaag cgcagcgagg cacttccgtc gacaccatcc aggtgcgata cctgctcgcg
     7861 cgcacactgg aatccttggg acgcatcgac gacacacttg agcactaccg ctggatcagg
     7921 cgggaagagc ccaacttcaa agatgtcgcg gaacgaatcg agcgcctcgg cggccgccgg
     7981 tccccatcgc ctcgcaacgc agaaggcacc ggagacggat cctcctgggt cgcacagctg
     8041 cagcgcattc tcggcgcctc caagtaagcg gccgcttcgc tcacccccac ctcccgatca
     8101 actgcccctc aagtttcacc ctgcgccgac cgatgatggt tcaggggagt gcctgcatgg
     8161 gtttagaaat cattgaaatt gtcgaagacg accaggggca ggccaagttg ttggatcaga
     8221 tccttcggca agcctcgttc cggaccaacg tcgcattcga tggcccgtcc gcgatgcagg
     8281 acgtgtggcg gatcaagccc tctttgatca tggccgacga taacctgcct ggtctgagcg
     8341 gtcgggaaat atgcaaacgc ctccggcagg acccttccac caaatacatt cccatcatcc
     8401 tcatgtccgg ctattcgtct gaagaacgtc gtgtcgaggc actcgatggc ggagccgatg
     8461 acttcatcgt caaaccctat gcagcgggtg aactggtcgc tcgcgtgagg gcgctgctgc
     8521 gccgatctca gcagggacaa ggacaggaag aggaacttgg tgaggatctt gccttgacgg
     8581 agagtctgta cgtggcagtg taccgcgggc agaagttgac gctatccgtt catgaatgga
     8641 aatcactgcg gcgactgacc aatacggtcg gaagcgtggt cccgcgcgaa gaactcagca
     8701 cgctcctctg gggggacgac gccctgatgc atgacgggga aatcgaccgg tgcatggagc
     8761 aattaaataa gaaactcgcc ggggagggcc cggctgcggg acacatcaaa atggttcccg
     8821 gcggctttcg cctgatcatc cctccctcag agaagccgga catcgtccag gctcaataac
     8881 gcgcctttgc gatcgccctc gctccacaga cggcctgcgg ccagtgcatc cagcaaccag
     8941 cgtaactcct cgggcactcg tctcgcccgg cgacgggccc gcttccagtc ccgttcgata
     9001 cgtgcctgca cggtgaggtt cagcttcttg ccccagcgcc ggaaatccgc catttcctca
     9061 taccgcccca agagggcctg aacctgtttg atccgtttca gtccatcctg tctggttgcc
     9121 ccttgtcccg gtagccattc cgtctggtag cggatcgtct tcagggcgag gcgcaacgca
     9181 tggaggcgtt ttcgacgagg cttctccacg gccttctcga tcagccgcga gaggacgcgc
     9241 tcatgttcgt gcctcatgac ttccagtcgg tgcttcacgg aaagattcgg gggagtgatc
     9301 atcggaagcg cctgtttcca gactgcctgt tcaatcttgc gataggtttg cgcgcgagtc
     9361 agtttgcgtt cccgctcgac gatccaggcc ccgacggcag tgatgtccga ctcgggcgct
     9421 tcaatcttca tgagatattg ccggaagact tgcaaggccc ggagccgact caaccgactc
     9481 acagtccggg ccatcaccgc ggcacggtca cgattattac agagttccag caatgcttga
     9541 agtctacggc agtgagtgcg aatcgagtgg atggtatcag cggtgccgtc gcccgccatc
     9601 gcgtggcgca tcaaacgaag caccgtggct tggtaggcgg ctgccgcctg gacgacatct
     9661 tgcccgaaac cggggggtgc ggtatgaata ggggaacgcg acttcctcat ggcagcctcg
     9721 aagcagacat gagaggtgcc ggccggagag cggcggtaag acttcgagta acgattgagc
     9781 ttgtaaccgc cacctgatca ccgattttct tcctgtagga ggatgcccga tggactgcct
     9841 cttctttcga catggtatcg cgatcgagcg tcaggagtgg agcgggcggg aacaggaccg
     9901 gccactgacc gacaaaggcg tagcgcgaac ccgagaatcc ggcaaggggc tgctcaatct
     9961 gcgcatcaag ccgacgcata tcttttctag tcccctggcg cgggcgcacg acaccgccgc
    10021 cattcttcaa tccttgattc gttccaaccc caagatccgg atctgcggcg aactcaatcc
    10081 aacagcggca ccccgcaccc tgtttgtact gctggatgcg ctgccgccgg acgccgtcgc
    10141 gctctgtatc ggccatgaac cccacctgag cctggcagcc ggaatcctgc tcacgggaaa
    10201 accctgcagc gccctctcgc tcaaaaaagc aggagcctgc ctgatccaca tcgaagagtc
    10261 cgtgaggccc gcggcaggac gactcgaatg gtggctgacg gctgcacagc tgcgcgccct
    10321 tgcatgatca ccagccgact cacaggccgg tccgacaagt aaagaacacc ggacgcgcat
    10381 cgtgaaagcg gacctgccgg gatactcgag actgctcatg gaaacaggca gggcatcgca
    10441 catgcgcacc gtgcgaggga gggcggagaa cacagaactc gaaccggccg acgctttagc
    10501 ccgcattatt catgacggtt cgtcgtgagc ccgtcggccg aaggctcgtc gcagcagcgg
    10561 atcggcgatt gcagcagaag cgctcatgaa taatgcgggt taggtgactt cgtcttcctg
    10621 gcttgtggtg acgaactgaa ccggccgctt gaacaccttc tcgaagagat cgctgcgacc
    10681 tcgcgccgcc cagatttcca gctccgcgtc acccgacacg tccaccgtga tcatgacggt
    10741 cttaccgatt ttgacatcga cgtttcggac caccgaaaac tgacttcgat ccaatccgtc
    10801 ggcaatacgg agcagggccg acagcacgtt gatgatgcgc tggtttcctt ccggcaaggc
    10861 ggtgaactca ccgtgcttgc gagtcggcac cgagcggcgg tgataccgcg cgatattcgc
    10921 caccagatcg atttcctcgg cggtgaatcc ggagagatcg ctgttcttga tcaggtagta
    10981 ggcatgtttg tgatgctggc gtgaattgat caaatatccg atatcgtgca ggatggccgc
    11041 gaactccagc cactcacgct cccgctgccc gaatccatgc aaagacgtgg tttgatcgaa
    11101 caggcgtaag gcgagtccgg cgacatggag cgcatgcgtc tcggagacat ggcaccggcg
    11161 agccagggcc aggatgttcc gcttccggac atcgggaatg tcccgctccg cctgaatgcc
    11221 ctcgtggtgg cgctgaatga agtcgtagat gatcccttcc cggatcgcct tgtcgcaaat
    11281 cgtcagctca tctttccgta acaggtcgag cagaattcga aacaccatcg tcgcaggaag
    11341 cagcgtatcc acgcgtttgg gatcaagccc cggcatggcc agccgggttt tcagcgtggc
    11401 gtcggccaga cgtttttcaa cggcggcaat ttctttcgcc gaaattttcg tgaggttcag
    11461 ctgctggaga gggcggcccg tgcgctgcag ataaatgact tcggccaggt tgcccgccat
    11521 cccggaagtg gcgatgatct gttccacgcg tttggtctta taggtaccga gcgcctgctt
    11581 gagttggccg gtcaccgctt cttccaattc gtgcagcatc gccttggagg ggggtgtctt
    11641 cgtcagatac aaatctttga gccgaatcgc tcccaacttg aggctgcgag cctgatagat
    11701 cgtttcccgg ttcccgacga tcacctcgac cgaaccgcct ccgatatcga tcacgagggt
    11761 aggcggctca gggagagcga cgctgttctg cacgccaagg aagattaagc gggcttcctc
    11821 tgcgcctgtg atgacccgga ccgtcaaccc ggtttgttgt gcgacatgat cgagaaattc
    11881 tccgccgttt cgcgcctctc gcaccgcgct ggtcgccacg ccttcgatcc gctcgtagcc
    11941 cttgttcctg gccagcgtga ccaggttccg gatgacttcg aggccgcgca tcatcgcctg
    12001 gtcggagagg cggcgtgacg tgaacacccc gtcaccgaga cgggtcatgt ctttgaagcg
    12061 gtcgagaatc ttataggtgt agtccggctg aacttccgcc aggaccatat gaatggaatt
    12121 ggtgccgata tcgaggacgg ccagtttggg catctacatg ctcgcagagg gagctacgtg
    12181 ccggatgttc ttgccttcaa ccatgtagac gacaagttcc gcaatattgg tggcatgatc
    12241 ggcaatccgt tcgagcgact tcgccacgaa ggtgagccgg atcgcgcggg taatggtccg
    12301 cgtgtcttcc agcataaagg agagcagttc tcgaaacaac tgctcgttca cctcatcgac
    12361 aaagtcgtcg tcggcgcaca ccttgcgcgc cagcaccgga tctgaattca cgaaggcgtc
    12421 cagacagtcc ttgaccatac gcatcgtcca attggccatg cgcggaatgt cgatgtaggg
    12481 cttcagctgc ggttcgccgt tcagctccag ggcacgctga gcgatgttgt cggccagatc
    12541 gcccatccgt tccagttcgg acgaaatttt catccccgtc gtcacgaagc ggagatcgcg
    12601 ggccgtcggc tgttgcaggg ccagcaactg gatgcagagt tcgtcgatct cgacatccaa
    12661 cgcgttgaca tcgtggtcct gcttgatgac atcgactgcc aggtcggaat cccgatccac
    12721 cagcgcctgc agggccaact gaatctgtga ttcgacgagc ccacccatgc ggagcaatgt
    12781 ctgcttgaga tgcgcgagat cttggtcgaa atgtcgttgc atggtcgggc tccttctcta
    12841 gcaggcgttc gtcgttcgtg aagcgtcagt cgtctttcgt ccgacaaaaa gagcctatag
    12901 cgtatagtcc gaaatggaga gagggtgtct gtgctaccgg ctataagcca ttagccatac
    12961 gctcctatcc acaacgagat acgtttcacg attcatgctt cacggatccg cacgtgacga
    13021 gagcgcagca ttgcgttcgt gttcaacatc ctgacaggtc atccaaaccg gccggtgatg
    13081 taatcttcgg ttcgtttatc gtgcggggtt gtaaagatcg tcttcgtatc gccgaattct
    13141 accagttcac ccatcagaaa aaatccgcat tgatccgaca cgcgcgcggc ctgctgcata
    13201 ttgtgcgtca cgatcaccac ggtgtaggta tccttcaagg tatagatgag ttcttcgatc
    13261 ttgccggtcg cgataggatc cagggccgaa cagggctcgt ccatcaggat cacctcgggt
    13321 tggaccgcca gcgcgcgcgc gatgcacaac cgctgttgtt gtccgccgga caatcccagg
    13381 gcgctttgca tcaaccgatc cttcacctca tcccagagtc cggcgccctg cagactatgc
    13441 tgcacgagtt catccagaga ccgcttatcc ttggtgccgt gcagacgcgg accgtacgcg
    13501 acattgtcgt agatcgattt gggaaacggg ttcgatttct gaaagaccat ccccacacgc
    13561 ttcctgagat cggtgacgtc gatggcgggg tcgtagatgt cgacaccgtc cagttccaca
    13621 cgccccacag cacgcgcccc ttccaccagg tcgttcatcc gattcagaca tcgcagcaag
    13681 gtcgattttc cgcaccccga cggaccgata aaggaggtga ccgaatgggt ccggaccgtc
    13741 agtgagaccc gaaaaagggc ctgccgttgg ccataataga agtcgagatt gcgaaccagc
    13801 acctttgcat cgacggaagt gccgtgcacc tcgtggggac gagtgagacg cggttgggga
    13861 acggtcggac gcagtgacaa ggccggcgcc gtcggcgaca gactacgagt ctggatatcc
    13921 atgcgcaatc ctttctgtga tcacactgcc gaggcggtaa atcgtcgccg taaacggttg
    13981 cgtagccgca cggccgccca attcagcaac acgaccacga ggatcagaat cagcgtggtc
    14041 atatacacca tcggcttcgc cgcctcgacg ttcggggact gaaacccgac gtcgtaaata
    14101 tggaatccca agtgcatgaa ctttcgatcg agatgcagaa agggaaggta ttcgtccagc
    14161 gggagggcag gggccaattt caccaccccg gtcagcatca agggcgccac ttccccggcg
    14221 gccctcgcca tggccaggat cagtcccgtc aggattcccg gtaacgcgca ggggaggatc
    14281 acatgccgga gcgtttcaaa tttggtcgca cccagtccga gcgaaccttc ccggaaatcc
    14341 cgtggaaccg cagacagtcc ttcctccgtc gccacgatca cgaccgggac ggtgagcagc
    14401 gccagggtca aagaagccca cagaatgccg cccgtgccga aggtgggatt cgggagcgcc
    14461 tcgggaaaca accattggtc gatcgtgcca cccagcgcat agacaaaaaa ggcgaggccg
    14521 aacaccccga agacaatgga cggcacaccg gccaggttgt tcaccgcgat ccgaaccagc
    14581 ctgaccagca ggccttgatt cgcatactcc cgcaaataga cggcggccat cacaccgaac
    14641 ggcatcacag ccaaggtcat cagaaaaacc atcaacaccg tgccgaagat cgccgggaag
    14701 atgccgccct ccgtgttcgc ttcacggggc tcagtggaga cgaacagcca cagactttgg
    14761 acataggcca ggagtttctc accccaatgc atcccgttcg gccgactgac ggccaacacc
    14821 tgatcgagag agagccgcac cgaccggccg gtagagacgg tcaagaacaa gacctcctgc
    14881 ccagcctgct tgtgtagccg ctcaagcgcc tcagtcttgg tgagatagtc tcgctccaac
    14941 gcggtaatct gatcgaggag gcgctggtct ttttcggcat cgccgggagc gagtcggccg
    15001 cccagcgtca acgcatgtcg ggcaagcctg gccttctcaa tccgatagtt gatggaaccg
    15061 atttcttctc gctccagatg ttcgatccgc cgacgtaact gattggcccg ctccaccagc
    15121 gtcttcaccg tcgtccatga actcccgctt gcgcttcctg tcgcggcccc ctccacgccg
    15181 accgtgatcc gaccgaaggc aggcccccac tcacgccgct cgacaagcgt gagatcctcg
    15241 ggatgcgcac tcgacacaat atccgcggca ttgatccaac gatattcgga cccgaacaca
    15301 tcccgattgc cgacgcgata ccgaaaccgg cgctgtccgg caggcaagtc cggcgtgatc
    15361 gaattgggga tgacttcttc tcccaccact tgaccgatca ctgcctcctg cgtcttcagc
    15421 gtcgtctctt gcaatgcacc gggccagaac tgtcccaggc cgttgatcat aatcagtccc
    15481 agcatgccgg ccaccatcaa caggctgagc gccacaccgg aggccgtcat ccagacgaac
    15541 agttctccgc cccgccagaa atccttcatg agcgtcgacc gttcacccat ggctgtactt
    15601 atcccggagc cgctgacgaa tgagttccgc tagcgagttg atgacaaacg tgacgatgaa
    15661 caagagcaag gcggccagga ataacactcg atagagactg ccgccatgcg gggcttccgg
    15721 aatttccacg gcgatattgg ccgacagcgt ccgaaaaccg ttcgcccagt tccaatccaa
    15781 gatcggagta tttcccgtgg ccatcaggac gatcatggtt tccccgatcg cgcgcccgaa
    15841 cccgatcatc acggcggaaa agattcccgg gctggcggac aacagcacca gatgcagaac
    15901 ggtttgccaa cgggtcgcac ccaacgcgag cgagccggcg atttggatct tcggcacatt
    15961 ggagagcgcc tcttcggcga tactgtagat caccgggacg atggcaaatc ccatcacgag
    16021 accgacgacc agggcgttgc gctgatcgta ttgaatgccg agccggctag agagccaggc
    16081 cttgacgttc ccgtcgaacc accaaccctc gattaaagga ttagcccaga gacacccggc
    16141 aatgccaagt ccaagaatag ggatcaacag caaggcttcc tgaccctgcg ggaggtgtcg
    16201 tttcacagat aacggacaga ggtaccaggc gaacgaggcc acgacgacca gcagtggcaa
    16261 catcagcgcc ataccggcca ccgccggaag cattcgttca aggagggggg ccagccagag
    16321 cccggcaagg aaaccgagca ccacggtcgg caaggccgcc atgacttcaa taataggttt
    16381 aattttcgcg cgcaggtcct gatgcatgaa ctgcgaggtg cacatcgccg ccagaatggc
    16441 aatcggcacc gcgagaaaca gggcgtacat cgtccctttg agcgttccga atgccagcgg
    16501 aagcagaccg agcttcggct ccacatcatc cgacccggag gaggattgcc acacatgcgc
    16561 cggctgatcg tacccctcgt accagacctt gccgaacaat gtctcaagcg tcacttcagg
    16621 gtaggcattc tgcacctgat acagcgacag ttgcccggcc ccgtccaatg cgatcaaccc
    16681 gtcgcctttc ggcgcgaatg ccacggcgcg gatcggaaac tcgctgttgg ccagccgcaa
    16741 caacgtttgc gaagaggtcg catggtgcag gaacagattg cccttcatat cgccggtcac
    16801 aaaccccttg acccgctgcg acggagcaaa cgcggtgacc ggcgcattgt gagcgcggag
    16861 ggtatggatt tggatcagtg tccaaccgga cggggcatcc gcgcgtcgca caagtcccca
    16921 ggtcgagacc gcaccgtcct cagacatgac caccaggcta cgatcgccgc tcagaaaccc
    16981 caacgtcgtg acggccgtcg aggatgcggc gacagtatag gcggccctca tctccggcga
    17041 ctccgtctcg gacaaggcga catgcaccac ctgtccgtcc gcggttccca catacagatt
    17101 ttcaagtaac gcatccagag cgagggccgt caccgcacct ttcggttgcg gcagttccgt
    17161 gatgatcgga tggtcgcccg ccacgtcatc cgccgcgatg cgcacgacga gcagccggcc
    17221 gcccttcgac aggagggcca gcagacttcc gtgatcattg gcccggtaag ccagccggac
    17281 gatggggcct ttcgtcagtg gaataggttg accggcgcga atatgcgggc gcttgcgtcg
    17341 ctcgccttga tcggtaaatt ccgtcgtggt gccgactttc aagaacagga cttcaccatc
    17401 ggctgtgccc acggccaacc gcggagcatt gccgcctccg gacgccatcg ccgtgatctg
    17461 gcggctcgtt acctgtgccg gcatctccaa cgggatgggt tggcccgatg ccagatcgaa
    17521 gaactgaatg gctcctgatg tgaacacctg cgcgatctca cgatgttcat cgaccgccac
    17581 ctgagccgga ccttcgtcca caaggagggc cggcacactc aagcgttgag tcaacttcgc
    17641 gctcggcgcc gtgaacagcg gggtgacttc gcgaaacaga aaaaagaaga tcccgagaat
    17701 gctgacgatg gtcgccagcc caccgacggt aataatggcc cgggcaagcc gatcgaacaa
    17761 tgttcggaga tgcacaggcc tcagcatcag tcggaatcgc tccgtgatca ttaccggatc
    17821 tggctcagct gcttctcagc caaggtggca tcgaccggga agtagccatc cctcagcacg
    17881 tcggattgcc cctccctgct gtacacgtag gcgagaaact ctttcacgat gggcgagagg
    17941 ggttttcccg gagcctggtt cacgtagatg tacaagtatc gccagagcgg atacgtgccg
    18001 ttccgcgtgt tcgcccgggt gggttcgatg aacggttggc cggccttcgc cgccagcgga
    18061 agcattttca ccccggacgt ggtgtagccg atcccgctgt acccgatgcc gtatcgatct
    18121 tcgctgatcc cttgcaccac cgaggcagag ccgggttgtt ccttcacctg atccttgaag
    18181 tccccattct tgagtgcatg ttccttgaaa aatccgtatg tgccggaagc agaattgcgg
    18241 ccatagagac tgacaggcgc cgcggcccag tctccggtca atccgagttg cccccactta
    18301 ctcagctcga cggcatggcc ctgccttcgg gacttggaaa acagcgcatc cgcctccgca
    18361 accgtgagtc ccttgatcgg gttgtctttg ttgacgaaga gcgccaaggc atccaccgcg
    18421 accgggtagg cagtcggcgg atacccgaac ttcgtttcga atgcatcaat ctcgctcgac
    18481 ttcatggtcc gcgacatggg accgagttga gccgtgtttt caatgagcgc aggcggcgcc
    18541 gtactggacc ccttcccctc gacttgaatt ttcacattgg gatattgttt ccggaatcct
    18601 tcggcccaca gcgtgatcaa attgttcaag gtatcggacc cgatgctgtt gatcgtcccg
    18661 gacacaccgc tgaccttctg ataaggtcgt aacgtggtct cagctccgtc ggactgctca
    18721 gcgcaggcag cgggcatgat gatcagcgca tagagcaacg aaaacacgag gacggtcgtc
    18781 aacggatgcc agctttccga ttcacggacg atacttgact cgcgacgcat atggtctccc
    18841 tcctgttggg agctacaata taagaaccgt cggtttcggt gagatcgcta ctctgttaaa
    18901 tagctgttaa ctcttctgac gacgtgggaa tccggctgtt ggttgcgctg agcagccgtc
    18961 tgctgccatg actgagcccc cagacaagaa ccgcaagggc tgcagattcg ctcgccggcg
    19021 acgcaaggaa ggcaaagcca ggccttgatc ggcacatgga ttagttgtct cgtcggcatg
    19081 cagccgttca gacggccggt ctgcatcccg ccaggtcttt ttgatgaacc catcggagca
    19141 gctttaaccg ggtgctgatg cccagcttct ggaacagatt ggtcaaatga tggcggacgg
    19201 tgatggtcga gatgtgaagg aggtcggcaa tgtctttgtt ggacaggcct tgtccgacca
    19261 acagggcgat ctcctgttca cgatcggtca agggagaatc aaggcgttca tgcaaggagg
    19321 gactcgcagg gtcctgacag acggtcattt ccctgctatc cgacggggtt gggtcattgc
    19381 gctcctgccg ccgggaggtt ccgggaagcc tgcggaggta gtcgacggtt gcgagcagga
    19441 tctctaccgg ttgaatgttc aatacaatgc tgtcgatgcc ggacaacaag agcccgctca
    19501 taccgtgtct gtattccatg tcgctgacgg cgatgattct gatcgtcgga gcggaaatcc
    19561 gcaccctccg caccagttct gcgacgtcaa tttctgactc aagcaccaca atgagcgccc
    19621 ggggcttctc gcgcgcgacg atctcctccg cgtccgatgc gttgatcgct tccccaacca
    19681 gcctgagatg cggctgagtc gtcaggacag tctgcaggcc gaggcgaacg agatcactct
    19741 tgcagacggt cgccagggca aacgaagaat cgggtggttc gcgttcgctc actgcttcca
    19801 tcattcctgc ccgagatcta ccgccctctg ccccgttaca cataggatga gcccgaaagg
    19861 cgatcgaggg catcctgcct cgtggctgcg ggttgtgaaa gcgtcaaccg ttcggcctcc
    19921 agccaatctt ggcgatcgtg cccatcctgc cgtccccggc gctgatacaa ctcataggcc
    19981 agtttctcaa tgcgtgttct gatttcctcc gcccctgcca aaccgccggc tgctcgcggc
    20041 ctccggtcgc ggaccggccg tgggcgggca gaagtcttct tgaccgactt gggtgggctc
    20101 cctgtctgct tattgtctgc gcttgacttg atccgtcgtt gtgccatcgc tgaatctcct
    20161 ggttgcatcg aactgaacct tccttcttcc gacagagagg catcacgtgg acaatctgca
    20221 cggcacgggt ctgtcaagat tttcggaaaa ccattccgcg atacgatggt gaggacgcgg
    20281 agtcgctggc cactcgcggc gtctatgggc aagagcctca cccaacactc tgtgttgcag
    20341 tcttgccggt cctccggctg tccttacacg ggatcggctc gtgcggactc gttgtcctgc
    20401 gcaaggtcag aagacgcgcc tgatcctgcg ctcacgaggt gaatcgtcat gaacggacac
    20461 aagaccgtaa gggtgaggtt ggcgctgcct tgctccgagg gcaggggcgt acgactattg
    20521 ctccatgaaa acatctcacc ctcctccatg ttgggaggcc agcataggag cgtgacattt
    20581 catgagactc accgtttctt taacatccgg ttaaaccgga ccattcctgg attattttaa
    20641 cgaagcctta tgcgctgcgg cgaaagtcag cggggctgtg gctgtcgaag taacctcttc
    20701 gcaatgcagc cttgaggaag atgaaacagg tcaacggtag ggtccgttgc tatgggttct
    20761 aggatcgttc aaatcattga agacgagccg ctccacgccg acctgttgga ccgcgcgttg
    20821 cgccaggccc aattcgcgac gacgcttgcc gcggacggtg agagtggatg gcgtgacgcg
    20881 cagcgactgc tgccttcgtt gattctgctg gacctcatgc tgccgggcct gagcggccat
    20941 gacatttgcc gactagtccg acgcacaccg tcgacgcgcc atatcccgat catcatgctg
    21001 acggccgtcg gaacagaagc agaccgtatt gccggccttg agatgggagc agacgactac
    21061 gtggtgaagc cgttcagccc gagggaggtc gtgtcccgcg ttcaggccgt gctgcgcaga
    21121 gtccatgaga cacgtcgtga ggaccctgtt ctgttttccg atacctccat cacaatggag
    21181 gggccttact tcgtcctgtc cttgcaagaa cggcaaatca ccgtctcgaa cacagaactg
    21241 aggctgcttc agtacctgct gcctcggaat ggggaattag tgcgcggcga tgaattgttg
    21301 aatcttccag gcgaggagct cgggagcacg agcagagaag aacttgagca ccgtgtgcgc
    21361 tttcttcgac gaaagctgga gaacagcggg acgggatcga ttgagatttt gccggggtct
    21421 cgttatcgtt tcctggccca tccgaggcca acctgacaca accggccgga ataccgcctc
    21481 attcggaacg ctgcggcact caaccacgcc ccccgacagg gacaggcttt ctctaggtgt
    21541 cacctgagac ccgccacgca gggatcagcc gacgcaggct aagaccctcg ccctcgacga
    21601 aatccgttcg atctcgtact caaagcctct tcgaagcaga atgatttctc cgcgtcggct
    21661 cagctcgtcg acggtcttaa agacctggtt ccagctgagc tccggcagca gggtcacgac
    21721 ctgctccagc gtcagggatt ttcgcaggca tacgagatcg aggatcagac cttcgctggt
    21781 gggggaaatg gatggatgca ccatggcacg cctccttgtt gggaatcctt cactcaccct
    21841 tgtcgcagac ccgggtaact ttggagtcgg cgatcacgtg cagacagtaa catggggaaa
    21901 ccgtatgtct atgcaatcaa cgccgcatca attctgagtt aactcgttcc agggatcgcc
    21961 tcgccgctaa atctcttcgc ggccgcagaa cgatcctctc ccggttccgt cgccttgcgc
    22021 cggcgaacaa cgcaccgcca ccacccgaat cgctccgagt gccaggagaa tggctccaag
    22081 cgagagcaac ttcatgtggt ccgcatcctt gaaccagagg gaaatgattt ccgtcaacat
    22141 gaccacgagg atcgtatcga cgatgaaggt gactttgacg cggccttctg agaaataggt
    22201 cagcgtggtc ttgaacacct ccaccacggc gagaaggatc aacgtatcga cgatgatctg
    22261 ccggagcccg atctcaaccg gagcatgcag cagcagtccg atatccagaa acgttttgac
    22321 gaccccgccg gtcagggcaa tgaggatcgt cagaatgagc aggctgagga ctgccttaat
    22381 ccctttcatc cagagttcgg tgagatcggt gtcgatggcg cgaccgagaa aatcggaaaa
    22441 gcgatgtgca cgtggttcta aattcgtcac aatcatcctg agaactcctt gttgtgaggg
    22501 atactttctg tcgaaaccca agcggctact catatttaat cagtatggac cacaggccaa
    22561 gaaagaggcc gacgagcgag accatggcga agtgaaacgc gtcatttgag gatgatgaca
    22621 tcaagaaatc ggcggccgac atacgcaatc ctcctggtta tgaaaagcat ggtacgcatc
    22681 aaagtcttac tcgtgatcaa ctatatcgag gtgttgttcc gggaagttca ctgcttggtt
    22741 acggcgttgt tacttccagc gaagcagaat gtcgcggcct atcggagcag aatcgaaggc
    22801 cgactccgga tagtgctcga ttctcaccct gagcacgagg ggcgtaagag ttaacaagga
    22861 gttaacatct cgctaatggg ccagcaattc ctacccggta tcctggcgcc agtgataaac
    22921 gacattcctt tggtattcga aaacagagca acttggtcgg ggaattcatc tctattcaac
    22981 ggagggctca gatgacgcga caggagcaga caggttccag acatcacctt ggcactcgtt
    23041 cgattctcac cgcagtggcc accgccgccg tgctgtgctt attcgcaggg tatgccggag
    23101 cggaacatgc aggcctcagc ggtaccggag gaccggcggt cgaccccgca atcgaaccct
    23161 atacgaatca taatggtctg cacggcaagc tcagcattgc tggatcggac accatgaggc
    23221 cattgatctc aaagttgtcc gcgcaatttt tgagcctcca cccagggctg cagattgctg
    23281 tcgagggtac cgggtcgagc acagccatcc gtgagttctt gctgggcctc tcctatcaac
    23341 gacgggggga caaggttcaa agtaaaggca cggccggctc gagcacggtc gatttgctcg
    23401 cttcttcccg gcaattgacg gaagacgagt ccaaagcctt tgtatccaac tatggctatc
    23461 gccccttgga ggtgcccatc gccatggacg cagtcgccgt ctatgtgaac aaggacaatc
    23521 cgcttcagcg gctgaccttg gcggaggtgg acgctatttt cgggaaagat cgtaaacggg
    23581 gcctggaatc catcaccaat tggagccaaa tcggattggg caactccgcg ttggccaaac
    23641 agccggttca cctctacggg cgagacaagc gatccggcac cagggagttt ttcaaacatg
    23701 tcgccctgaa ggacggggag ctgctggacg acgtgatcga acaaccaggt tcggcgtcgg
    23761 agatcattgc gatcgcccaa gatccgctgg gagtaggata tgccggcgcg ggcttcgtca
    23821 tctcggccgt gcgccaagtc cctatcgccg cccagtccga aggaccggcc ctattgccgt
    23881 cggtcgagac cgtcacttcg ggtgcctatc cgttgggccg ctcgttgtac ctctacgtga
    23941 agaaaagccc gacagataaa ctggatccgg tcgttgaaga gtttctggcc ttcgtgaaca
    24001 gccgccaggg acaggaaacc gtggcgcgag cgaacttcta tccgttgacc tcgacgcagg
    24061 tggcgaagaa tcgccaagac ctggggctgg ccaaaggggc gatggtcatc ctgccgtctc
    24121 cggatctgca agtcgccgaa caaccaacac ggtgaccctc tttttcatcg cgtctgcgac
    24181 gagggatcgg atagggagcg cttgactcaa aaggcgctcc cttttctgtt tattttgcta
    24241 tgatgcgcgt gattcggttc tcaactccca gggaccgccg atcgtctttg ccccacgaat
    24301 ctgagactgg agcacatgga atgaagccca cattgcgtcg aacagtcatc atctccgtac
    24361 ttcttgccgc cacggcgcac ttcggctgca ccactccgcc gccgccgcgc acctcatatc
    24421 aggatcctat caccgccatt cggctctacg tggatgaccg tgcccggtcc ggccaccagc
    24481 atccggccga tgtctcatca gaacagatcg ccaaggtgct gggcggccta cgtgtcatcc
    24541 cgcgatccgg attcatcggc tccttgatca caggacaggc tcaagccacg cccgcctttt
    24601 catccactga gatccaggcc ttggccccaa gactcagccg cgcgttgact gaggccaagc
    24661 ccgatgaact ggtgacgttt taccgtcgct tttccgactc cagcaccgga ctggccatca
    24721 cgtccggcgg gatgttcgtc caggacgggt atctggtggt tgtcctcgcc aacaaccgca
    24781 cgctcccgac tgatgggatg aatcaaaaca tggtgacgga tttcgatccg gtcgacagtc
    24841 cgctcgttcc tatctcacgt acgagcttcc gagttgaatt cgctcacccg tcggccctgg
    24901 ctgcgcctga caaacggccc tcgtggccct atatcgacga gggccgcatt ctcgccatcg
    24961 acctcagacg tctgtcgact gagtccaaat cgccgtcagt acctccggcc ccgtgatgcc
    25021 tttgaaatct taaaatcgtg ccggctcttc gattagcggt gagcctggtc ggtttcgtca
    25081 cgaaccttcc tgacgaaacc gacgggacgc caagcgtgat aggcgcatga agccccgaga
    25141 ggccgaagca tgcgtgagac cgtctgttga ggctgacagg ggagagccca ttcgggggca
    25201 tgccgctgag ccgtaggccg gcttatccca gcgaccggtc ggcggctgta ggagaagctt
    25261 gcgtgaagaa tgaggaccag gttgatctga ataaagcgag actgccaatt ggcaggaaaa
    25321 agaacggaag aggcaggcgt ccggaacttg aagtccgaac gcccgccaac ggctacttgc
    25381 tcgttagttc cagaaccatt gtgtcatcaa tcgcagctgc gatgcgtcga agtcactttg
    25441 ggcacggttc gcgaccaagg tactgttcaa accatccttg aacatccaat ccgccactac
    25501 tctgaaatgg gtatcgggct cccacaccaa acccagattc catgtgcgtg attgcccgtc
    25561 cggcgcacca gtgagtgatt tgaagcctcc atagtactcg ccgtaacgaa cgtacggagt
    25621 caacatacca acatcggagt agcgccaggt gtagtacccc tggacatagc cacccaccac
    25681 attgctttcc tcaatgaagt tggtctgggc gtttcgttta gggccgcgac cgacggtgaa
    25741 ttcagccagg aggccccaag gttgaggcgg agtccacacg taagcggtga cgcgttgatc
    25801 cttgatatcg caaccgcctt ccgaagtcag ggggctgata cacctcgctg cgccggcacc
    25861 tccgggttga ctggctccct gcacggaaaa cactccgtgg tacccgagca cgccggcctc
    25921 gagcaaccgc ccgttcggaa attggaaggg ataggagacc ttggcgccga tgtgtttatc
    25981 cttattcaac tcgaaccggc tcctgccttg tccgttgtag accatgaccc caaacacgcc
    26041 atagtcgccg ggaccgttgt gataggcagc caattgagca aaccgctctt gtgcgatctt
    26101 gggactccag tagtaggcca cgcccaaatc gcgttcgccc ggcgcgccgc tctggatgga
    26161 ttcgtgacgg tccatttcct gccggttcgt gctcgaccgg tacgtatcga aggaattggg
    26221 aacgcgttgc aaaccaaagc gtaagcggtg caccttgtcc ttggtgaggt agtaatccgc
    26281 ataggcgtcg atcatctcgt tatttgccgg cgtctgttgc cctccttcga gcgcaaactg
    26341 ggtgaagaaa gctacccgtt ccgacacttg cccttgaaac accaaccgca tccggcggat
    26401 gtaaaattca ttcccctgct ggtcgccgaa gctgtcgccc aaaggattgt cgaacaggcg
    26461 attcgtggcc gccatgttgt acttgtattg aatgtagccg gtgatgcgaa tgcgctcgta
    26521 ccaacgagga ttgccggcca tctgcaattc cgcggactgt tcgaacacgc ggcgttcctc
    26581 ctcggccttg agacggatcc agtcttcctg ggtgatcacc cctttctcga gtaacaggct
    26641 ttcgagggag gtactctcga tcgagaggcg ctgccccgat gtttgtcggt cggtaccgtt
    26701 cgcggtgggt gtcacctgcg gtaccgtctc catggcggag agcgcctgga tcgattgagc
    26761 aggcccctgg gcgacgactg tcggcgcggc atcgtttgcg ccggccgatc ccggcagcgg
    26821 caagtagaac agcgctccgc agaggccgag cgtcgctgca gaccgtttca gaacatggat
    26881 tctcccgtac ttcattcatc cctcctcata gtgtgatgct gttacccatt cgggccatgg
    26941 tctgtgctta cagtgaaaat gatgaaggca aacgatttga cgcggcgagt atccctcgat
    27001 ttcgtgacac ctctgtcacc gacgtattaa gactttgtaa acacactatg aggatgtgcg
    27061 gcggggagaa aatttacaca catgtaacag gctgattact ccgcaacaat cgagcgccgt
    27121 tatggtgcgg catccgtgcg tggagggttc atgattacaa gcggcacaaa gacgcctctc
    27181 cttctcgtaa gcgccgacaa agtttcgtcc cgatctctcg cacaactgct ggagaccaac
    27241 ggctacagtc tgagcgtggc gtcgacactg acgtccgctc agggagaaat ccggcgactc
    27301 tccccggcac tgattcttgt ggatcgagac atcctcgccc aagaaaccgg ccgccgagac
    27361 cagttctcac ctaccatccc catcattgtc cttcagccgt tcgacaagtc atgcggcccg
    27421 gatgaatgcc tcaccgaact ggaggctgga tacgaccttg tgttctgttc tcctcactac
    27481 cgtgaattgc tcgcgcacat ccgggcgata cttcgtcggc acaagatgat atcggcatcg
    27541 ccggccgtac tccgcgtcca aggcctcgcg atggatacgg ctcgccatga ggtgaccgtt
    27601 gggggagccc tcgtcgcact cacgccgaaa gaatttcgca tcctccatca atttctcctc
    27661 tccccgggtg tcgtgttgtc gaggcaggat ctgctcaatc gtgtctgggg cgaggactat
    27721 gctctcgaag aacatgcgct ggatgttcac atccattcgt tgcgccagaa gatcgaggcg
    27781 gatccttcca agccgatatt catcgtgacc attcgcggga tcggatacaa acttcagacc
    27841 acctgaccgt tttcttccgg ctttctcttt cccataaacc actcatgttg ccgcggaccg
    27901 ctgctgagtt caccccgatg tgagagtgaa cgggtagagc cacatttcct cgaatgagtg
    27961 gaggggtggc aagtgcgccg tcaaggcagg taacagaaga ggtgatgggt gaggaagagg
    28021 ggagagttgg cctgctccta cctggcaggc cgtcgatgga caagtaccga tgcggtgacg
    28081 agttaaaact cgtccaccga caccggctgc aacagatccc gtactgaaag gacccccacg
    28141 atcgcgccgc tcttcaggac gcccagatgg cgcgtatggt gatgttgcat gagatcggcc
    28201 gcttccgtga tcgaacgccg ttcatccaaa ctgatcaccg ggctgctcat gatcgattcc
    28261 acgggaacaa aatgcaccgg cctgttttca cccacgacct ttcgcacaat atcggactcg
    28321 gtcacgatgc cgactacacg gtcgttctgc tcgataaaca cgctgccgac gtcgagatcg
    28381 ttcatcacta aggctgcctc cgactcggaa ataccagccg gcaccgtcac cagtttcttc
    28441 gtcatgagat cactgactgt caccatactg tatcctcctt gttgaatgtg tgtcatcggc
    28501 ggctgtctct caccttgccg atgctctaac agggaagata ctcctgttac gttccaggat
    28561 ggttaggcga ccgtaaattc tatgttacga atgggctcga agtggaatgc agggaggaca
    28621 ggcgcggcac aggcgaggat cgcccatgcc gcaccgtgag aggaatgccg aggaacgact
    28681 actctttcac tacgaagtgg acactgcgat tcttctgcca gcagaccgga ttgtgctgaa
    28741 gacacaacgg ccggtctttt ccgtaactgg tcgtatcgac ctggagctga ctcatgccca
    28801 acgattcgag gtagcgtttc acggagaggg ctcgccgttc acctaacacc agattgtatt
    28861 cggaagtccc gacttcgtcg caacgccctt ccagcaacac cttggtgatc cggttgtctt
    28921 tcaatcgctt ggcattggcc tcgaccgccg tgagcgcatc gtcccgcaag acgtattggt
    28981 caaaatcaaa caacacatcg gggaacggaa tgttctgctc cccggtcgca tttcgcgcgg
    29041 ccatctccac cgatcgcaac ggcaccgggg ccggttccgg cggggcgggg atttcctgta
    29101 ccggagccgc agccacacgc tcctccgcgc ggggcttcgg tggagtcgcc gtcactcctc
    29161 cgctggttcc cgatttgctc gcgcagccgt caagaaacag ggggccggcg acggccagca
    29221 atccgagcag gacaaacggg cgcaaacgta tcatgaaaac ctccggcgct agatgggttc
    29281 gtggagtgga cgaggcacag aataatcaaa tcgaacgaac agggcaagtt acttcttcgc
    29341 ggcacagcaa tcgtagacgc gtcgcgaatc cgacaacggc tcccaccgta agatctgcaa
    29401 cgcatcgtcc acataggccg gcgtgcgcat tccgttgagc acggacgtga cacccggtgt
    29461 gctggcgaga atccacagcg ccttctgcga caagctcgcc tttcgtctct cttcgggcaa
    29521 cagcggatcg atggttcgat gcaggtcttc gcgcgctctc cgaagcg
//
LOCUS       DAORRQ010000052        29522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_101_length_29522_cov_24.737639, whole genome shotgun sequence.
ACCESSION   DAORRQ010000052 DAORRQ010000000
VERSION     DAORRQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 29522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101_length_29522_cov_24.737639"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PK224_15100"
     CDS             <1..933
                     /locus_tag="PK224_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17122.1"
                     /translation="QAPLKEWPRLSNQPEPPLCEALGYADSIFIPGRDELLNRTANYS
                     GVEATIVANILCHLTYGSEPGPMPDPALQPLISLNPDVLAIMPNLWLNNSAERNLTVL
                     LNRLPQESPIYSSLVDEKEALMRSRFEASLALDALRLVKGEISGASNLPDIDLAIIED
                     ASKTCLLFELKWFIEPAEVREVIEKTEELEKGVRQLLTLKRAFEENHLQFLDKLQIDS
                     TYALYLVLASENWIGHSNVQNPMVSIIKSSHVLEKLKTTKDLRETGEWLRKRQYLPTR
                     AIHYEAMETEFTIKNWTLNWCGIKPLLTDIFFPA"
     gene            complement(1030..1725)
                     /locus_tag="PK224_15105"
     CDS             complement(1030..1725)
                     /locus_tag="PK224_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRB17123.1"
                     /translation="MPVTPHIEKHFTATATVRDIVIGMADGLTVPFALAAGLSGAVAS
                     SGLVVTAGLAEIAAGSIAMGLGGYLAAKTDLEHYASERLRELRETQHIPEREAEEVSE
                     IFRGYGLRDEQIAPLIETLQANPTQWVDFMMRFELGLEEPDPTRARISAWTIALSYVV
                     GGLIPLLPYMVFSDIHTALWWSVAVTPVALFVFGYVKSGFTGVPPWRGGFQTVLVGGL
                     AAAAAFGIARLLG"
     gene            complement(1744..2001)
                     /locus_tag="PK224_15110"
     CDS             complement(1744..2001)
                     /locus_tag="PK224_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17124.1"
                     /translation="MPPKRKVPRQSGRNRSVGQKRKGAATKSGEAGMTRRLQDLVERI
                     DVVLPEVAARVAALEHLLLELKLCTQEDLRSAREFVRIQEA"
     gene            complement(2323..3567)
                     /locus_tag="PK224_15115"
     CDS             complement(2323..3567)
                     /locus_tag="PK224_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17125.1"
                     /translation="MPIDHSGATTNGGLVLSATGSQWRQSARSLLGRLCPMFVSMLLL
                     VVVPGTSAADDRWHPGIYVKIEDWQLRSPAQMEAIYEELRNTPQLRGIKVILLWGRYE
                     SRDSRGGASRYDFSQLDEILARLGALENKHLIVALAWREFKSAKGASEILPNDLQGRR
                     AWNVDPNRAHTQYEYLWAYRMSNQPGKYAYNLKLWHPTILGRLDAFLAALAAHIDQHP
                     NLTQISTTESAIGEPVTSFVEEGGSAQRQYDGQLAVIRAMKKYFVQSDVVPDFNYSRE
                     HVAAMVPILQAEGIGLGSSNSNKSKGLILQPPSGAPGVLTYYPRLSGTVILAPEIQGD
                     DYRSTYGHDTPPDHPRYESIYLRVRDELKANYTVMQRNTPYWLGDASTPSMLKFIQSY
                     PAIVNDRTGAGGLNHVKPKSVR"
     gene            complement(3860..4717)
                     /locus_tag="PK224_15120"
     CDS             complement(3860..4717)
                     /locus_tag="PK224_15120"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRB17126.1"
                     /translation="MASAQELVQSCSNVFTLPEIYYRVRDVVDNPDSTMDDLAKVLKM
                     DPAISARVLKIVNSPLYGVPKQVDTVTRAVNLLGMQAIRDLVTATTIGRSFSGMTVQI
                     MDLSAYWRKSVLCALMAGKIAKSCGIDDSERFFIEGLLRDIGHLVLYQTIPERAQSAL
                     IEAGNLGSPLAEVEQSNFGCDFTEVGAELIHSWGMPSQIEQAIRHQLCPDEAGDYALH
                     ASIVHLAGVVADHNELHPSVAPKELSFHPAALQSTRFDVSERPALLNEAQEQLQETVK
                     LFSPVAMAA"
     gene            complement(4925..5500)
                     /locus_tag="PK224_15125"
     CDS             complement(4925..5500)
                     /locus_tag="PK224_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRB17127.1"
                     /translation="MAVESFMLSLGTSAPAFTLRDVVSGEVYQLDSFAGSSALLVMFI
                     CRHCPYVVHVEQELAGIGRDYQGTGLGMIAISSNDPKSYPDDAPPRLKEMAERLAFTF
                     PFCCDDTQEVAKAYRAACTPDFFLFDRYRRLVYRGQLDDSRPGNNKPVTGRDLRAAIN
                     AVLGGKPVNPSQKASVGCSIKWRPGNAPVYA"
     gene            5712..6134
                     /locus_tag="PK224_15130"
     CDS             5712..6134
                     /locus_tag="PK224_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17128.1"
                     /translation="MQFDAALAAQDTLQESETELGADWDRAVELESTFSSNAGCTARE
                     AYEQLLALADRHPDAHRFQAFCIYITWQQVTEETIARHFQTGMTLSETYLASPEGKDP
                     RHLAYVTELLESFRAGLGLDEEDDIVVEFRKDTPKGGD"
     gene            6272..7039
                     /locus_tag="PK224_15135"
     CDS             6272..7039
                     /locus_tag="PK224_15135"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17129.1"
                     /translation="MADNDKHRARIFLHRGDLLQARAAWEAAVKDDRLTGTQRELSNS
                     LGNLGNTYALASELEKADACYKEVLAIQRGEQDPHAIAHTLVNLGNLYIGADRPEKAR
                     PYYLEALDLLKPLNDHRALGILYHNLAMEEARQHQWDESIRLFTQALDSHRVVGNEEG
                     LAGTYSQLGKTFLDSGRTVEAERCFNNASEHFIKLGNPAGEAAVLRELADLYEQRQDR
                     VAAIRCVERLHHLALGTGRAPAAADRERLARLRATRS"
     gene            7106..7423
                     /locus_tag="PK224_15140"
     CDS             7106..7423
                     /locus_tag="PK224_15140"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17130.1"
                     /translation="MDISAFRQMVEKNPKGFLGRYGLGNKILQEGGSVEEAAKHLTVA
                     TQLDPTHVASHLALGRALVSLGRKEEAKPVLKAGIDAAVSGRSNGGVDLVPELQQLLR
                     TLG"
     gene            7440..7640
                     /locus_tag="PK224_15145"
     CDS             7440..7640
                     /locus_tag="PK224_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17131.1"
                     /translation="MTLDAARQRIESAIAQYGQHAAMAIELVINEVRSDMGRDAVNEL
                     IDEFDLELMYNIAPMESDYSNS"
     gene            7799..8878
                     /locus_tag="PK224_15150"
     CDS             7799..8878
                     /locus_tag="PK224_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17132.1"
                     /translation="MPLIWCSISGHGYGHAAQVVPVLNALGRLVPDLSAILRTTVPAG
                     FFEPRLDIPWQLKVGQQDIGCIQDGPLKIDVSRTWAAHSVLHSDWQQKIEAEAALIRS
                     AGPACVLSDISHLGIAAGAATGIPTIGLCSLSWDLVLEPFADPARREHATILCQIREA
                     YAKADCFLRVAPALAITAFHPVREIGPIAEPAVPQAAALRKAIGAGQSERVVLVGFGG
                     IALRAFPFEHMEAMDGFRFVVDGAVPDGLTRVLSLAALGMTFKAVMASVDMILTKPGY
                     GTIVEAVALGIPVVYVRRYNFPDEPPLVDHLHRYGRGAELSLVDFLQGRWNAAIEQAF
                     ARPLSSTPPPLSGAQDAAHILKDYV"
     gene            9063..9668
                     /locus_tag="PK224_15155"
     CDS             9063..9668
                     /locus_tag="PK224_15155"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB17133.1"
                     /translation="MEAASRQAASTIDPKLLARVAKGDQQAFGQLYDQSSTLLFTLAY
                     RILNDRDEAAELLQEVYLEVWRKIAKYDVGRGSPIAWLVTLTRSRAIDRLRARASRGQ
                     HVVADSFEHPLVSRTPDVSPNPYEAREDTELRQLMAKAILELPLPQQQAIEMAFYQGL
                     THTEIAAKLNQPLGTVKTRIKLAMAKLRASLQQTLPQGEGV"
     gene            9665..10681
                     /locus_tag="PK224_15160"
     CDS             9665..10681
                     /locus_tag="PK224_15160"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRB17134.1"
                     /translation="MTHEELEDAVPLYAIGALERSERQAIEAHLLSGCAACHAALKDY
                     QTVASLLPFGLTPATPPNTLKAKIMMAPTPIAGAVEAEQAGPKSSLEPGEWMNHLFPP
                     IAPARSLPFRLAMGFAAAVLIVGGGYLAWSSYTQTSQRSGEIQQLHAAVQQETARVAT
                     LQSELQQREQTIGTLKSEIEQRTTEVAELRDQILQREAELDDAHTQLTQNESSLQRLA
                     RQNEEFAGLFKNPSSKVVSLAGSDMAKSAGAFLLFDPSTKKAWLYAYNLPALPSGKVY
                     QLWAIDDKPVSAGVFGLDAGLKARMLIRNMTEFSRMKKFAVTVEPDGGRPEPTGAIYL
                     IGQI"
     gene            10814..11302
                     /gene="tadA"
                     /locus_tag="PK224_15165"
     CDS             10814..11302
                     /gene="tadA"
                     /locus_tag="PK224_15165"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRB17135.1"
                     /translation="MLPLDDLDTRFMQQALTLARSAPLIGEVPIAALLVHEGIVIAQA
                     HNLRETRQDPTAHAEVIVIQDAARHMGSWRLIDTTLYVTLEPCTMCIGAIVLARIARL
                     VFAATDPKAGACGSIMNIPPEPRLNHLVEVVGGVCAEESQTLLQDFFRQLRKDAARRE
                     IT"
     gene            complement(11299..12063)
                     /locus_tag="PK224_15170"
     CDS             complement(11299..12063)
                     /locus_tag="PK224_15170"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRB17136.1"
                     /translation="MSGLLIPSRDSSPSPALSREDVHVWFCRLARHDGRRAALAALLS
                     RDEQTRAARFAFERDRHRFILSHGLLRAILARYVGREATQIEFATGAHGKPAVSGQSC
                     AVQDIQFSLSHSGAYAVVAVAAGRAVGVDVEVRRPDVDALKLAERFFAAGEAQQIIQA
                     QGDAQQRLFYRSWTAKEAYLKGRGVGLSLGLDRFEILFDDGSPLAHVRMTDSGALDTN
                     WQVQSLSLADDVSGALAVEGQAWQVRMCDSSTDLFR"
     gene            complement(12095..13792)
                     /locus_tag="PK224_15175"
     CDS             complement(12095..13792)
                     /locus_tag="PK224_15175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HRB17137.1"
                     /translation="MMKMYRFLLFLLRDARSMMVLMVLTGLLAGLSSVGLLAVINKLI
                     NGAGATSELFAVAFIGLAVLKVVSNYLSQLLLVTFAQKTILNLGMDLCWKVVRAPYRT
                     LERRGAHEILATLTDDTNAMAWAVNGLPGLAINVAILAGCSLYLAWLSWQAFLGVVIL
                     AVFGLVGYRQLYNRVLRSSLAVRDSKGVLFEHFRSLTEGMKELMLHRGRRETFVDSDI
                     RQAAEALRHHNLVTTKQYLTTDSWTQVLFYGLIGVILLLFPRLLSLSGEALTGYAFAM
                     LYMIGPMWGLLGMVPTLSRGQVALEKIESLGLALEEGGREGGAERAVIHGTQSLEFSE
                     ACFSYEPKGEDERSFSLGPLNVTVRTGELVFIIGGNGSGKSTFVKVLTGLYLPQQGEV
                     RLNGEAITPATQDWYRQHFAAVFSDFYLFKKLLGLDPALIESQAEGWLKTLRIQHKVT
                     IRDGEYSTINLSQGQRKRLALVTAMLEDRPFYVFDEWAADQDPQYKEVFYGELLPELR
                     ARGKGVIVVTHDDRYFHVGDRVLKLDEGKILEIPNSTASTGSHRATPVFKPVIRSSA"
     gene            complement(13997..23359)
                     /locus_tag="PK224_15180"
     CDS             complement(13997..23359)
                     /locus_tag="PK224_15180"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRB17138.1"
                     /translation="MVRHPSLSIPPGETLLDVLRWRAEHQPDQSAYIFLRDGISPDTV
                     LTYRQLDAKARSIAGHLQSRYAAGDRLLLVYPPGLEFVQAFWGALYAGLVAVPVPPPD
                     AFRARVGVTRVQRIAEDAGAAGALSTNQILELVRAQGTTIPLEQWVICDASRPDSTAQ
                     WRSIHPLPSTLAYLQYTSGSTSAPKGVMVSHGNMTAQSRCITEAGCYDADSVTLSWMP
                     HFHDYGLVKGIVQPAWIGRPAYLMSPLTFLKRPLRWLEAIQRYHVTHSGAPNFAYRRC
                     VETIAAEDRARLDLSGWQVASCGAEPIAHDTIDRFAEAFAPAGFRREAFFPAYGMAEY
                     TLLISLKREGVAPTVLSLDPATLERGVVSEAKAGIAPVLQVVGCGAPVGDTRVVIAHP
                     ETLSRCAVQQVGEIWLAGASTTQGYWNNPEETARTFGATLRDTGEGPFLRTGDLGFVR
                     DGEVFVTGRLKDLLIVRGRNHYPQDIERTVEQSHRLFRGGGAAAFSVQDAGEEVVVVV
                     QEVERQATALAIDELAAAIRSAVSEQHDLHVFSIIFIKAGTLPKTSSGKLQRRACRDQ
                     FLTGQLSIIGKSVLPAPLSQAQTTVVNLEDLRALSSDARRRQIEQAVQGMIADRLGCS
                     RETIVHDGPIHLLGLDSLMAAEVSHRVEESFQVPLSLQHLLGGATLGDLAATIEAGML
                     REGSELIAAGGQNLTTENVAPLSENQAALWFLSQLAPDSAAANVSVILPLPFDVNQAA
                     LKQALDRLGERHATLRTTYETQDEVPVQRIHARLPLIWTVIDASRWDWARLRREAMEA
                     AAIPFNLAHGPLWRASLFKGKQQAWLLLVAHHIAVDGWSMIHLVEDLKQDYASDGSLP
                     QDGSNGLGSQPAPYGAFIGWHRALLESAEGGRLAQYWQARLAGELPNYDMLYDRPQPT
                     VEPSRYAWQTIHIDRALTERLKAFAQAEGTTLYAVCLTALHALLYRYTNVEDTAVVTP
                     VFGRSRSRFARTVGDFVNMLVLRETVQVDSTGRELLALTKRTLLEALDHQDYPYARLV
                     SDLRPARDRQRAPLGQILFVLQQFRLLSELDGRMSGSSSSVSESCLPPWEAYVIPQQS
                     GQFDLCLELAESEQGVTGYFEYKDDLFTPDRMARMQQHFVRVLEGLVADPAVQIGSLP
                     LMSEAECRETVLTWGQSPELQAPAQCLHRQIEAQVLRTPDAIAVRQGDQELTYRELNA
                     RANRVAHYLRRRGVAPGVVVGLCLERSLNLIVGMLGILKSGGAYLPLDADYPTDRLEY
                     MLRDSQVRVLVTQQDQLARLPATNPHTICLDSEWDQIARFPDDAIEGTDAPENLAYVI
                     YTSGSTGQPKGVMIEHRSIANYVRAITEIAGVHSRDRVLQFASMSFDTAAEEIFPCLT
                     TGATLVLRTQMMIDSVSGFLDRCRDWQLTLLDLPTAYWHEVVTRMELEQLAFPESVKT
                     VIIGGERVLPQIVQRWAKLVGRTVRLLNTYGPTETTVAVTWSDLTGLGLQEELREDVP
                     IGRVIAQTSVYVLDRHRRPVPVGVPGELYVGGVGVARGYRGRPELTDTKFIPDPFSSR
                     PRARLYRTGDLVRWRPDGQLDYRGRVDRQVKIRGYRIELEEIEAVLNRHPDLERAVVE
                     VREDQPGDKRIVAFMVPRPQNRLGLVQLREQLRSQLPAHMIPSAFVEMETLPLTVNGK
                     VDRAALRVATDSRASKVDLTSEFLAPRTPTEQVLADIWGEILHVKDVGVHDNFFELGG
                     HSLLATQLVSRVQALFRVVLPLRQVFERPTIATLAEVIKRSQEGVTQQAARDGRMMTK
                     AAGGAPLPLSFAQERMWFLYQLSPTGAAYNIPASVRLHGPLNRAALRWGVAELVRRHD
                     ALRTTFATVDGQARQIVHTSLDPPWVEEDLRGLPREMREARALELATAEARRPFDLGQ
                     GPLLRILLVQLGEEDHVLVVSTHHIISDQWSYGVIARELVKCYNAFCAGKPVAIQPDL
                     AIQYADFAQWQRTWLTGSVLAEQLAHWKSKLTDLPVLALSADRPRLPIHSFKGDQVSL
                     DLSWALVNRLKQLSVREGVTLYMVFLAGFFGLLHRLTQQRDLVIGTPIANRNRLEIED
                     LVGTFVNTLVLRTDVTGELTFRELLQKVRDLSLDAYAHQDIPFEKLVEELRPDRSQGG
                     LPLVQVLFNFANTPFARTEFQHLSWTPYEVSRGAAQLDLGLSIDPLASRKAYLEFNTD
                     LFDRTSAERWLAEYRQFMEVIAEHPEEKVGRVAILTEQEQHRILHEWNATDRPVERET
                     CFPQLFEAQVGRTPDAIAVVCEGAELSYGELNRRANRLAHRLREQGVGPDVVVPVFLE
                     RSPELLISLLAVMKAGGAYLPLVPGLPIRRLAAMIEASHAAVLVTDSTLLGGLPQHQL
                     RVVCLDGEAESLTRYPEKDPAPLAKPEHLVYVLFTSGSTGQPKGVEIEHGALVNFLRS
                     MQQEPGISSRDVLLAITPLSFDIAGLELYLPLLVGARIILAGRLQAMEGAWLQRELDQ
                     GAVTMMQATPATWRMVLQSGWQGGRQVKVLCGGEGLPRELAQELLARAGSVWNVYGPT
                     ETTIWSTLERVRTAERTISLGRPIANTQVYVLDLNREPVPVGIPGELYIGGMGLARGY
                     RGAPQLTAERFVSSPFRAGERLYRTGDQVKWLPDGRLEYIGRIDYQVKLRGFRIELGE
                     IEAVLADDPTVKQAVVIVREDAPGDKRLVAYVVAREGRRCDPQALRRALRDMVPDYMV
                     PAAIVPLNEFPLTPNGKVDRGALPAPVTEPVHDSAQPIEPRNRVELQLVAIWEQVLGI
                     TPIGVRDNFFALGGYSLLALRMFSAIEQTFSIRLPMAVLFQAPTIEQLADVLAGEGCT
                     VRWRSLVAIQPEGKNPPFFAVPGVGGNVLVFARLAKLLGDAQPFYGLQARGLDGKEKP
                     FMRVEEMAAHYIEEIRSIQPHGPYLIGGTCTGGLAAYEIAQQLMAQGEQVILAVMESW
                     HPRSYLTHWSRPPYLLWPILFVWMKITTYLRLMRRLPIQEWPSFWRGKLKRIWNLMHH
                     TEAAEHQDEFLYKDQVTYATFHAVARYDLKPFRGQVLNVIASKHPLTNSSDDTRLVFG
                     ESVMGMSRTIYLPAEDSGRLFVAPHVQELAHHLKTFWQEAWATLRNKPDDQGKGPSSR
                     AA"
     gene            23639..23726
                     /locus_tag="PK224_15185"
     tRNA            23639..23726
                     /locus_tag="PK224_15185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23673..23675,aa:Ser,seq:cga)
     gene            24170..24304
                     /locus_tag="PK224_15190"
     CDS             24170..24304
                     /locus_tag="PK224_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17139.1"
                     /translation="MFEQTEAALVMTLLALGGIALMADESIGTTCMQMVKWLRTRGRR
                     "
     gene            complement(24301..25806)
                     /locus_tag="PK224_15195"
     CDS             complement(24301..25806)
                     /locus_tag="PK224_15195"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HRB17140.1"
                     /translation="MLITPEPAARSSYRLSKSRYLSGLQCHKRLYLEIHAPALATKPD
                     AATQAILDMGTDLGELARRRFPGGRLVTAGYRQSQEALAQTAELLQDSTVSAIFEGAF
                     QFDQVLIRVDVLERVGVNESGQPTWRLVEVKSSTKLKPIHVEDLTIQSYVLEGAGLVL
                     DDICLMHVNTQYVFDGVELDLGQLFSLRSLNETIRPRLPDVPSRLTSMHAALSEMSAP
                     PIAPDEHCHSPYECPFWAHCTQEKPPRWIYHLPGSSKTVIQLRELGVETIDEIPDHVT
                     LTQVQRRVRDNREWIGEGLRSALEKIVYPVHHLDFETFMPAVPKFGDTRPYQVIPTQW
                     SNHIEHPEGRLDHAEYLCRDGRDPREELAVTLLDSLGGEGSICVYSSYERSVLERLAE
                     DFPSLRKDLKRVIARLWDLHIVIRDHYYHPAFEGSYSIKAVLPAVVPSLSYADLAIRD
                     GGVAACEYARMIFTVSDWIEKAQIEEALLRYCERDTLAMVELRKELKKRAG"
     gene            25902..27239
                     /locus_tag="PK224_15200"
     CDS             25902..27239
                     /locus_tag="PK224_15200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRB17141.1"
                     /translation="MANGVAQIRRSVMTLALPVTVSSLLQRTEGIVAVFLVGGLGAIP
                     IAAVGLGQLLAFIATTLVSGLSVGTNVIIAQQWGARRYEEAGQASRHFLGLSIFVSFA
                     LALLGLSANGLIMQLLGAQPEVIGLALPYSNLIFLVIPFTVLLAVLSSILQGTGDTKT
                     PMYAMIMVNVLHIAIAYPLIYGQWGLPAFGVKGAAVAVGIAEATGSVYLLLRCRAILK
                     PSKRLRRDLLRTIWQVGAPVSGERIVQQAGILLYTKIVLIYGTVSYAAHQVGLSIESL
                     SFLPGYGFAIAAATMVGQSIGAGKYTRAKLENWEANRLATFIMSAMGIVFFFFPYALL
                     RAFTSDEAVIELGTTFLKIVALLQVPLALTMVLAGSLRGAGDTRFIMIATMIGMWGVR
                     IPIAFVAGYWLTMGVFYVWLAMIADWTLRMALMLWRYRSERWKSIRVLRSSTT"
     gene            complement(27245..28612)
                     /locus_tag="PK224_15205"
     CDS             complement(27245..28612)
                     /locus_tag="PK224_15205"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRB17142.1"
                     /translation="MKQIMVVGAGSVGGFFGAHLAKNNPNVSFLLRPRTLEAVKRHGL
                     MIKSAKGNFTVHPPAASDPRELAPPDLIILAVKAYDLDEVMAQLEPVLTERTVMLTLQ
                     NGIDTEDRIIARVHRDSVVGGVAFIYSKIVAPGVIEHYKRGGVAIGELMGHKSERVLQ
                     IAEIFKQAGISCQLSDDIRKSKWEKMCWNCVFNPLTVLIDDKVAKALDHPEMAGVIRQ
                     IVGEVMAVSAAVKVPLAPDMAEKVVKWTQELRDIHTSMYDDWKAKRPTEIDYLNGYIV
                     RMGRELGIPTPVNEALTAMVKAITEREPAGPGVVRIDGAVVQPVSLTRAALAQLPQAQ
                     HVEDISQLMPSMRGRAIRVNGLLDIPALAVDADHVTFHSVDGKYAATLTLQQARDFGL
                     LLYELDGQPLPDGKGGPYRLVTPGLGDLCANVKAVGRIEVRAGSGKDTRPSVRPPECS
                     TEGSR"
     gene            28703..29434
                     /locus_tag="PK224_15210"
     CDS             28703..29434
                     /locus_tag="PK224_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRB17143.1"
                     /translation="MGTGSTLGLIRQKLASARSVTVLTGAGISADSGVPTFRGVDGLW
                     RNFRAEDLATPEAFARDPRLVWEWYNWRRELIASKRPNPAHDAVAQMEQRFERFWLIT
                     QNVDGLHRDAGSRRLSEIHGNIWMVRCTQCRRVTENRDVPIAILPLCSNCNGLLRPHI
                     VWFGEALAEQDLDASEAALQSSDLCLIIGTSGLVYPAAGFGSIAKQAGAFVVEINLDA
                     TAQSGIVDAALQGRAKDIVPLLLQA"
BASE COUNT         6187 a         9383 c         8340 g         5612 t
ORIGIN      
        1 caggcgccac ttaaggaatg gcctaggctg tccaaccaac cggagccacc tctctgtgag
       61 gctttaggct atgctgatag catcttcatc ccaggtcgtg acgaactcct caacagaacg
      121 gccaattact cgggcgtgga ggccacaatt gtggcaaata tcttgtgcca tctcacttat
      181 ggaagtgagc caggaccaat gccagaccct gcattgcagc ctctcatctc actaaatccc
      241 gatgttcttg cgatcatgcc aaatctctgg ctcaacaact cagctgagcg aaatttgacg
      301 gtactactga acagattgcc acaagaaagc cccatatact catcactggt ggacgaaaaa
      361 gaagcgctga tgaggagtag attcgaagct tcactggctt tagatgcctt aagattagtc
      421 aagggtgaaa tatccggagc ctccaatctc ccagacattg acctagcgat aatcgaagac
      481 gcaagcaaga cgtgcctgct ctttgagctt aaatggttca tagagccagc ggaagtacga
      541 gaggtaatcg aaaaaacgga agaactcgaa aagggagtgc gacaacttct tactcttaag
      601 cgtgcatttg aggaaaacca tcttcaattc ctggacaaac tacaaataga ctccacctac
      661 gccttatacc tggttcttgc ctcagagaac tggataggcc atagcaatgt acagaacccg
      721 atggtttcga taattaaatc ctcccacgta ctggagaaac taaaaactac caaagatttg
      781 cgcgaaacgg gggaatggct caggaagcga caatatttac cgactagagc aatccactac
      841 gaagccatgg agacagagtt cactatcaaa aactggaccc tgaattggtg tggtataaag
      901 cctctgctaa ccgatatctt tttcccagcc tgaggaaata cagagttgtt tctaaaagcc
      961 agtccaaccc cgtgccagga gcaacctagc tccttttgac cccacggcta ttagagagaa
     1021 cttgccacct caaccgagca ggcgggcgat tccgaaagcg gccgccgcag caagaccccc
     1081 gaccagcacg gtttggaacc cgccgcgcca aggcggtacg ccggtaaacc cgcttttcac
     1141 gtagccgaat acaaacagag ccacgggagt caccgccacc gaccaccaga gcgcggtgtg
     1201 aatatcggag aaaaccatgt acggcaggag cggaataagt ccaccgacga cataggacag
     1261 cgcgatcgtc caggcgctga tccgcgcgcg cgtcggatca ggctcctcga gccccagctc
     1321 aaaccgcatc atgaaatcga cccactgcgt cggattggcc tgaagtgtct caatcaacgg
     1381 ggcgatctgt tcatcgcgca aaccatagcc gcgaaaaatc tcggacactt cttcggcctc
     1441 ccgttccgga atatgctgcg tctcccgcaa ctcccgcagg cgctcagacg cgtaatgttc
     1501 cagatccgtc tttgccgcga gatacccacc caagcccatg gcaatcgagc cggccgcgat
     1561 ctcggctaac ccggctgtga ccaccaggcc ggatgaagcc accgcgccgg agaggccggc
     1621 cgccaacgca aagggcaccg tcaacccgtc ggccataccg atgacgatat cgcggaccgt
     1681 cgcggtggcg gtgaagtgtt tttcaatatg aggagtcacg ggcatgtgag cactccgagc
     1741 tgcctatgcc tcttgaatgc gcacgaactc ccgcgcgctg cgcaaatctt cctgcgtgca
     1801 gagtttcagt tccagcaaga gatgctccaa ggccgccacc cgggcggcta cctccggcag
     1861 caccacatcg atccgctcca cgagatcttg caaccggcgg gtcatcccgg cctcgccgct
     1921 tttcgtcgcc gcgcccttcc gtttctgtcc gaccgaacga ttgcggcccg actgccgtgg
     1981 cactttcctt ttgggtggca taggtccctc ctggttaggc tgcttgtgta cgcgccttcg
     2041 tggcgccggt caagcggagc aggtcgagaa cggcatgtcg atgcgagaaa taaaacctca
     2101 agcgatagtt aaagctctca ggcaggcagg tccaacagcc ggaattgtca agggatggct
     2161 gataagccga tcaacgccgc caagactctc caatcttcca taaatcgaac ttcgaacagg
     2221 ctagagcagc atgggaactt gcatggcaca taaattatta cttcaagttg tattgtgccg
     2281 atgagggcgc ggaaacagcc ccctagttca tggctggggc aactagcgga cggacttcgg
     2341 cttgacgtga ttcaaccctc ccgcccccgt cctgtcgttg acgatggcgg ggtaggattg
     2401 aatgaatttc agcatgcttg gcgtggaggc atcccccaac caatagggag tgttccgctg
     2461 catgacggta tagttggctt tgagttcatc gcgcacccgc aggtaaatcg actcgtaccg
     2521 tggatggtca gggggagtgt cgtgaccgta ggtacttcga tagtcatcac cctggatctc
     2581 aggcgccagg attacggtgc cactcaatcg cggatagtac gtgagcactc ccggtgcacc
     2641 tgacggcggc tgcaggatga gacctttgct cttgttgctg ttcgaactcc cgagcccgat
     2701 cccttctgct tgcagaatcg gcaccatcgc agcgacatgt tcccggctgt agttgaaatc
     2761 cggcaccacg tcactctgca caaagtattt cttcatggcg cgaatcaccg caagttgtcc
     2821 atcgtactgc ctctgcgcac tccccccctc ctccacgaaa gaagtcacgg gctcgccgat
     2881 cgcggattcg gtcgtggaga tctgtgtcag gttcgggtgc tgatcgatgt gcgcggccag
     2941 ggcggccagg aaggcatcca atcggccgag aatcgtcgga tgccacaact tgagattgta
     3001 ggcatacttg cccggctgat tactcattcg ataggcccac agatactcgt actgggtatg
     3061 ggcgcggttt ggatcgacat tccaggcccg tctcccctgc aggtcgttcg gcaggatctc
     3121 tgacgctccc ttcgcacttt taaattcacg ccatgccagc gccacaatga gatgcttgtt
     3181 ctccagtgca cccaaccggg ccaggatctc atccagctgg ctgaaatcgt accggctggc
     3241 tccgccgcga ctatccctgg attcatatcg cccccacaac agaatcactt tgatacctcg
     3301 cagctgaggc gtgtttctta attcctcgta aattgcctcc atctgcgcag gtgaacgtaa
     3361 ttgccagtcc tcaattttga cgtaaatgcc tggatgccat ctgtcgtccg cagccgatgt
     3421 gccgggaacg accaccagaa gcaacatcga gacgaacatc gggcagagcc ggccgagaag
     3481 actcctggct gattgacgcc attgtgagcc cgtggcgctc agcacaaggc caccgttggt
     3541 ggttgctcct gaatgatcga taggcataag tcgatgttcc ctgtgtcccc gcccgtcagt
     3601 cgatggaact ctcgctggac gacgaacctt gtcggtccct cgccattctg aagggagaac
     3661 cgaccattgt ctgtagtcac ccctgggaca gggatgatcg cacaggagat cggcttgact
     3721 gggggcagat gagaagggag cggtcagcgc ggactcgacg agttggaggg gaaaatcaag
     3781 aacaggatcc gcctccgccg gcgatgcact cgaggttcga ggcagctcgc cggcggtgag
     3841 atcgcgactc tgcctgaagt caggcggcca tcgctacggg actgaacagt ttcaccgtct
     3901 cctgcaattg ctcctgcgcc tcgttcagta aggccggccg ctcgctgaca tcgaatcggg
     3961 tggactgcaa tgcggccgga tgaaacgaca actccttcgg agcaacggag ggatgcaact
     4021 cattgtggtc ggcaacgacc ccggcgagat gtacgatcga ggcatggagc gcatagtcgc
     4081 ccgcctcgtc gggacacagc tgatgtctga tggcctgctc gatttgagac ggcatccccc
     4141 aggaatgaat cagttccgcg cccacttccg taaaatcgca cccgaagttg gattgctcga
     4201 cttcggccag cgggctgccc aggttgccgg cttcaatgag cgccgattga gcgcgctcgg
     4261 gaatggtctg atacaacacc aggtgcccga tatctcgcag caacccctcg ataaagaacc
     4321 gctcactgtc atcgatgccg caggatttgg cgatcttgcc ggccatcagg gcgcaaagca
     4381 cgctcttgcg ccaataggca gacagatcca tgatctgcac cgtcatgccg ctgaaactgc
     4441 ggccgatggt tgtcgcggtc accaggtcac ggatcgcttg catcccgagc aaattcaccg
     4501 cgcgggtcac cgtatcgacc tgcttgggaa ccccatacag cgggctattc acaatcttca
     4561 ggacgcgggc ggaaattgcc gggtccattt tgaggacctt cgccaaatca tccatcgtcg
     4621 agtccgggtt gtccacgaca tcgcgaactc gatagtagat ctcagggagg gtgaaaacgt
     4681 ttgagcagga ttggacgagt tcttgcgccg atgccatgat agacgatcct ccttggtgcg
     4741 cgttgcgcgt gcgccctgca cagggggccc ccggctatct ttatcggttg cggcctgtct
     4801 tttctgaagc ggagcgaagc aagcggcgtc ccgccgtgag cattcgcccc ctgccgaccc
     4861 attcggccca acacccgtgg caacagaagc ctcaactgcc ctctggggat cggctgtata
     4921 ccgttcaggc gtaaaccggc gcgttgccgg gcctccactt gatgctgcag ccgacgctgg
     4981 ctttttggct gggattcaca ggctttccgc ccagtacggc attgatcgcg gcacggaggt
     5041 ctcgaccggt gaccggcttg ttgttgccgg ggcgtgagtc gtccagttgg ccgcgataca
     5101 ccagccgccg ataccgatcg aacaggaaaa agtccggcgt gcaggcagcc cggtaggcct
     5161 tcgccacctc ctgcgtgtca tcgcaacaga acggaaaggt gaacgcgagc cgctcggcca
     5221 tctccttcag tcgcgggggc gcgtcgtccg gatacgactt cggatcgttg ctgctgatcg
     5281 caatcatccc gaggcccgtg ccctgatagt cccgcccgat cccggcgagt tcctgctcca
     5341 catgcaccac ataggggcaa tggcggcaga tgaacatcac caacaaggcg gatgagccgg
     5401 cgaaggagtc gagttgatac acctcaccgc tcaccacatc gcgtaacgta aacgcaggcg
     5461 cactcgtgcc cagcgacagc atgaacgact cgacggccat atccacctca ccatattctg
     5521 tctcacacgg gtttgacccc gtcgcctcac cgcccattca ctctacaagt ctcgcgccga
     5581 cgactcaagc gcgagacctc attgacctgc ttttcactcc ccgcgtatgc tgagacctga
     5641 ggtgcctgca tcttcacgac gggcaccacg caaagacggt ccactgctca cgtttcaaga
     5701 aagaatcatc tatgcaattc gatgccgctc tggccgcaca agataccctc caggaatcgg
     5761 aaaccgagct cggtgccgat tgggaccgcg ccgttgaact tgaaagtacg ttctcttcca
     5821 acgccggttg cacagcccgc gaggcctacg agcaattgct cgcgctggcc gatcgacacc
     5881 ccgacgcaca tcgtttccag gccttctgta tctacatcac ctggcaacag gtcacagaag
     5941 agacgatcgc tcgccatttt caaaccggaa tgacgctctc cgagacctat ctggcctcgc
     6001 ctgaggggaa ggacccacgg cacctggcct acgtgaccga gctgcttgag tcatttcgcg
     6061 ccggcctcgg cctggatgaa gaagacgaca tcgtggtgga atttcgcaaa gacacgccga
     6121 agggcggcga ctaacccaag ccgtgaaacg tgactcgtga aaagtaaaac gtttaccgct
     6181 tcaaagctaa agagaacgca tggccactac tgcccatatt tccttgtaca ttccacgttt
     6241 cacctttcac gtataacgtt ttactcaagc catggctgac aacgacaaac accgcgcacg
     6301 catttttctc catcgtggcg atctcctcca ggcacgcgcc gcctgggagg cggccgtcaa
     6361 ggacgaccgg ctgaccggga cccagcgtga actctcgaac agcctcggca acctcggcaa
     6421 cacctatgcc ctggccagcg aactggagaa ggccgacgcc tgctacaaag aagtgctggc
     6481 gatccaacgt ggtgaacagg atccccacgc catcgcccat acactggtca atctcggcaa
     6541 cctgtatatc ggcgccgacc gacccgagaa ggcccgtcct tactatctgg aagccctcga
     6601 tcttctcaaa ccgctcaacg accatcgcgc cctcggcatc ctctaccaca acctggccat
     6661 ggaagaggcc cggcagcacc aatgggatga gtccatccgg ttgttcacgc aagcgctgga
     6721 ctcccaccgg gtggtcggca acgaagaagg cctggccggc acctatagtc agctgggaaa
     6781 gactttcctc gacagcggcc gtacggttga ggcggaacgt tgtttcaaca atgcctccga
     6841 acatttcatc aagctcggca atccggccgg cgaagcggcg gtcctccgtg aactggccga
     6901 tctctacgaa caacgacagg acagggtcgc ggccatccgt tgcgtggaac ggctgcacca
     6961 cctggcgctg ggaaccggac gcgcgcccgc ggccgccgac cgcgaacgcc tcgcgcgact
     7021 ccgtgccacc cgtagctgaa ccagcgaggg tgatggtaga atcccgctcc atcacaacag
     7081 acaatcacgc ttcacaagga gcctcatgga tatcagcgcg tttcgccaaa tggtcgagaa
     7141 gaatcccaaa ggattcctgg gtcgctacgg cctcggcaac aagattttgc aggaaggcgg
     7201 cagcgtcgag gaagccgcca agcacctcac cgtcgccacc cagttagatc cgacgcatgt
     7261 ggcgtcgcac ctcgccctcg ggcgggcact ggtgtcactc ggcaggaagg aagaggccaa
     7321 acctgtcttg aaggccggga tcgacgcagc cgtctccggg cgatccaacg gcggtgtgga
     7381 tctggtgccg gaactgcaac aactcttgag gacgctcggg taggacggtt ggaggatgta
     7441 tgactcttga cgcagccaga caacggatcg aatctgcgat cgcacaatac ggccagcatg
     7501 cggccatggc gatcgaactg gtcatcaatg aagtacggtc cgacatggga cgggacgccg
     7561 tgaacgaatt gatcgacgag ttcgacctcg aactgatgta caacatcgcc ccgatggaat
     7621 ccgactactc gaacagctga gcccggatgg ggatgttacg cacagccctc gcatggtgct
     7681 caaaaagacc atccaacgag gccgcagagg cggaagaccc ggagacgtac gttcagaggt
     7741 acgttgagga ggattccgag tcgagaacga cgttgggggt ctttttcagc agcctgccat
     7801 gccgctgatc tggtgttcca tctcaggcca cggctacggg cacgcggcgc aagtagtgcc
     7861 ggtgctcaac gcgttgggac gattggtgcc ggatctcagc gccattttgc ggacaacagt
     7921 ccccgccggc ttcttcgaac ctcgcctcga cattccctgg caactcaagg tcgggcagca
     7981 ggatatcggc tgcatccagg acggccccct gaagatcgat gtctctcgca cctgggccgc
     8041 ccactctgtg ctgcacagcg attggcagca gaaaattgag gctgaagccg cactcatccg
     8101 cagcgccgga cccgcctgtg tcctttccga tatctcccat ctcggcattg ccgccggcgc
     8161 ggctacgggc attccgacca tcggcctctg ctccttgtcc tgggatctgg tcctggagcc
     8221 gttcgcagat cccgcccggc gcgaacatgc gacgattctt tgccagatcc gcgaggccta
     8281 tgccaaggcc gactgcttcc tgcgtgtggc gccggctctt gcgatcaccg cgtttcatcc
     8341 tgttcgcgaa atcggcccta tcgcggaacc ggccgtccct caagctgcgg cgctccgtaa
     8401 ggccatcggc gccgggcagt ctgagcgggt cgtgctggtc gggttcggcg gcattgccct
     8461 gcgagcgttt cccttcgaac acatggaggc gatggacggc tttcgcttcg ttgtggatgg
     8521 cgctgtcccg gatggactga ctcgcgtcct gtcgctggcc gccctgggca tgacatttaa
     8581 agcggtcatg gcgtccgtag atatgatcct cacgaaacca gggtatggca cgatcgtcga
     8641 agccgtggcg ctcggcatcc ccgtcgtcta tgtgcgacga tacaatttcc ccgacgagcc
     8701 gcccctggtc gatcacctgc atcgatatgg tcgcggggca gagctctcac tcgtcgattt
     8761 tctccaggga cgatggaacg cagccataga acaagccttc gcccgccccc tttcatcgac
     8821 gccgcctccg ctgtccggcg cacaggacgc cgcgcacatt ctgaaagact acgtatgagg
     8881 tcgtgccggc tgatccttcg tgacactgcc ctcgatcccc gctgaagacc atcacgctgc
     8941 cgtgaacggc tgtgctacaa ctgccgacgg attctcacat ccgattagtc gtttttcacg
     9001 tagagtggta tagtgtgccc tattccgatc cggagaattg cgcccgcgcc caggccgact
     9061 gcatggaagc tgcctcccga caagctgcat cgacgatcga ccccaaactc ctggcccgtg
     9121 tggccaaggg cgatcaacag gcattcggcc aactgtacga tcaatcgagc accctgctct
     9181 ttaccctcgc gtaccgcatc ttgaatgatc gcgatgaggc tgcagagtta cttcaggagg
     9241 tctacctgga ggtatggcga aaaatcgcca aatacgatgt cggccgtgga agcccgattg
     9301 cctggctggt gacactcacc cgcagtcgag ccatcgatcg gctccgtgca cgagcatcgc
     9361 gcgggcaaca tgtcgtcgcc gattcgttcg aacatcccct ggtttcgcgc acacccgatg
     9421 tcagcccgaa tccgtacgaa gcccgcgagg ataccgagct acgccagctg atggcaaaag
     9481 ccatcctcga gctgccgctg ccgcaacaac aggccatcga gatggcattt taccagggcc
     9541 tgactcatac ggagattgcc gccaagctga accagccgct ggggacggtg aagacgcgca
     9601 tcaaactggc gatggccaag ctccgcgcat cgttgcagca gacattgccg cagggtgaag
     9661 gggtatgaca cacgaggaac tggaagacgc ggtccccctt tacgcgatcg gcgccctcga
     9721 acgctcggag cgacaggcca tcgaagccca cctgttatcg ggatgcgcgg cctgccatgc
     9781 cgctctcaag gactatcaaa ccgtggcgtc cctgctgccg ttcgggctca ccccggcgac
     9841 acccccgaat acgctcaaag ccaaaatcat gatggcgccg acaccgatcg cgggtgcggt
     9901 ggaagcggaa caggccggcc cgaagtcgag cctggaaccg ggcgaatgga tgaaccacct
     9961 gttcccgccc atcgcgccgg cccgctcgct gccgttccgt ctggcaatgg gatttgccgc
    10021 ggctgtcctg attgtgggag gcgggtatct cgcgtggtcg tcctacacgc aaacctctca
    10081 gcgttcgggc gagattcaac aactgcacgc cgccgtgcag caggaaaccg cccgtgtggc
    10141 caccctgcag tcggagctgc aacagcgtga acagaccatc ggcacgctca agtcggagat
    10201 cgagcaacgg accacggagg tcgcggaact tcgcgaccaa atactgcagc gggaagcgga
    10261 gttggacgat gcgcacaccc agctgaccca gaacgaatcc tcccttcaac gcctggccag
    10321 gcagaatgag gaatttgccg gactgttcaa gaacccctcg tcaaaagtcg tgtcgctggc
    10381 cgggtccgac atggccaaat cggcaggcgc cttcctgcta ttcgacccgt caaccaagaa
    10441 agcctggctc tatgcgtaca atctgccggc cttaccgagc ggcaaggtgt accaactctg
    10501 ggccatcgac gacaaaccgg tgagcgcggg cgtcttcgga ctggatgcgg gactgaaagc
    10561 tcgtatgctc atcaggaata tgacggagtt ttcgcggatg aagaaattcg ccgtgaccgt
    10621 ggaaccggac ggcggacgcc cggaacccac gggcgcgatt tatttgatcg ggcagatctg
    10681 accggccggt gactcgacct tccgctcacg cctcctctcc cggtgccatt ccacacacag
    10741 aacgtgctag catgcaggcg ccttttcgac tgccgtagcg ttcagcccct gacaggattc
    10801 atccgacgac acgatgctcc ccctcgacga tctcgatacc cgcttcatgc aacaagccct
    10861 gacactcgcg cgttcggcgc cactgatcgg tgaagtgccg atcgccgcgt tattggtaca
    10921 tgagggcatc gtgattgctc aagcccacaa tctcagggaa accaggcagg acccgacggc
    10981 tcatgcggaa gtgatcgtga ttcaagacgc agcccggcat atggggagct ggcgactcat
    11041 cgacacgacc ctctatgtca cgctggaacc ctgcacgatg tgcatcggcg ccatcgtgct
    11101 cgctcgcata gcgcggctgg tcttcgcggc caccgatccc aaagccggcg cctgcggatc
    11161 catcatgaat attccccctg agccgcgact gaaccacctc gtggaggtcg tcggaggagt
    11221 ctgtgctgaa gagagtcaga cgttgttaca ggacttcttt cggcagctga gaaaagacgc
    11281 agcccggcgg gagatcacct aacgaaacag atctgtactc gaatcacaca ttcgaacctg
    11341 ccaggcttgg ccttcaacgg cgagcgcccc ggagacgtca tcggccaatg agagggactg
    11401 cacctgccag ttcgtatcga gggctccgga gtcggtcatc cgcacatgag cgagcggcga
    11461 cccgtcatcg aataggatct cgaatcgatc cagcccgaga gacaacccca ccccacgccc
    11521 tttgagatag gcctccttcg ccgtccaaga gcgatagaac aatcgttgtt gcgcatcccc
    11581 ctgtgcctga ataatctgct gcgcttcccc tgccgcaaaa aaccgctccg cgagcttgag
    11641 cgcatccacg tccggcctgc gcacctctac atccacgccg acggcccggc cggctgccac
    11701 cgccaccacg gcatacgccc ctgaatggga aagactgaat tggatatcct gcacggcaca
    11761 ggactgaccg ctcacagccg gcttgccatg ggctccggtt gcaaactcaa tctgcgtggc
    11821 ttctctgccg acgtagcggg ccagaatggc gcgcagcaaa ccgtgcgaca ggataaaccg
    11881 gtgacgatcc cgctcgaagg caaaccgggc cgcgcgggtc tgttcatcac gcgagagcag
    11941 ggcggcaagg gcggcgcgtc ggccgtcatg ccgggcaaga cggcagaacc agacatgcac
    12001 atcctcccgg ctcagagcgg gcgacggcga agaatctctg gagggaatca gcaatccgga
    12061 catgggttga gatacacgag gcgcagaccc tgcgttacgc cgaggagcgg atcaccggct
    12121 tgaacaccgg ggtcgctcgg tgcgagccgg ttgaagcggt cgagttcgga atttccagga
    12181 tcttgccttc gtccagtttt agcacgcggt cacccacatg gaaataccgg tcgtcatggg
    12241 tgaccacgat gacacctttt cctcgcgccc gcaactccgg caagagctca ccgtaaaata
    12301 cttccttgta ctgtggatcc tgatcggccg cccattcgtc gaacacataa aacggtcgat
    12361 cttccagcat ggccgtcacg agagcgaggc gcttccgttg gccctgtgac aggttgatcg
    12421 tcgaatactc gccgtcgcgg atcgtgacct tgtgttgaat ccggagcgtc ttcaaccagc
    12481 cctccgcctg actctcaatc agcgccggat cgagccccaa cagtttcttg aacaggtaga
    12541 aatcggagaa caccgccgca aaatgctgcc ggtaccagtc ctgggtcgcg ggcgtgatcg
    12601 cctcgccgtt cagcctgacc tccccctgtt gcggaaggta cagtccggtg agcactttca
    12661 caaaggtcga cttcccgctg ccgtttcccc cgatgataaa aaccaactcg ccggtccgga
    12721 ccgtcacatt caacggcccg aggctgaacg accgctcatc ctcgcctttc ggttcatacg
    12781 aaaaacacgc ctctgagaac tctagggatt gcgtgccgtg aatcaccgcg cgctccgctc
    12841 cgccctctcg accgccttct tccaatgcca gccccaaaga ttcgatcttc tccaacgcca
    12901 cctggccgcg gctcagggtg ggcaccatcc cgagcagacc ccacatcggg ccgatcatgt
    12961 agagcatcgc aaacgcatat cctgtcagag cctcccccga caacgacaac aatctgggaa
    13021 agagcaggag aatgaccccg atcaatccgt agaaaagcac ctgtgtccag gagtcggtgg
    13081 tgaggtattg cttcgtcgtc accaggttgt gatggcggag cgcttcggca gcctgccgga
    13141 tatccgaatc gacgaacgtt tctcgacgcc cgcgatgcaa catcagctcc ttcatgcctt
    13201 ccgtcaggct gcgaaaatgc tcgaaaagga cccctttcga atcgcgcacc gccagcgacg
    13261 accggagcac ccggttatac agctgccggt atccgaccag tccgaacacg gccaggatga
    13321 ccacgccgag aaaagcctgc cacgagagcc acgcgagata gagtgagcag ccggccagga
    13381 tcgcgacatt gatcgccaat cccggtaatc cgttgacagc ccaggccatg gcgttcgtgt
    13441 catccgtcag cgtcgccaga atttcatgcg cgccgcgacg ttccaacgtg cgataggggg
    13501 cccgcaccac tttccaacag agatccatcc cgagattcag gatggttttc tgcgcgaagg
    13561 taacgagcaa cagttgggag aggtagttcg acacgacctt cagcaccgcc aggccgatga
    13621 aggccacggc aaacagctcc gaggtcgccc cggcgccgtt gatgagtttg ttgatgacgg
    13681 cgagcaggcc cacgctggat aacccggcca acagaccggt caggaccatc agcaccatca
    13741 tgcttcgggc gtcgcggagc aagaacagga gaaaccgata cattttcatc atggtgaggt
    13801 gactgctgtc ccgttggcct taacccggac tattcatgaa tacctgctcc ggcgtccgat
    13861 cctctcgccc ggcgaggctg ttctcgttcg gcctcctcga cggactgcaa gtacgcctgc
    13921 gtcggcctca cgtgcgagca gcctcggcgg gcttccgtca tgaacgcctt gcgacggcat
    13981 tcatgaataa tccgggttaa gcggctcttg aggaaggccc ttttccctga tcatccggtt
    14041 tgttccggag cgtcgcccag gcttcctgcc aaaacgtctt gagatggtgc gccaactcct
    14101 gcacatgcgg cgcgacaaat aaccgccccg aatcttcggc cggcagatag atcgtacggc
    14161 tcatgcccat cacggattcg ccgaagacca accgcgtatc atcgctggaa ttcgtcagcg
    14221 gatgtttgga ggcaatcacg ttcaagacct gtccgcggaa cggtttcaaa tcgtaacgcg
    14281 ccaccgcatg gaaggtggca taggtcacct gatccttgta cagaaactcg tcctgatgct
    14341 cggccgcttc ggtatggtgc atgagattcc aaatccgttt caacttgcct ctccagaacg
    14401 acggccactc ctggatcggc agtctccgca tgagacgaag gtacgtcgtg atcttcatcc
    14461 acacaaatag gatcggccag agcaggtagg gaggtcgact ccaatgcgtc aggtaggatc
    14521 gcgggtgcca ggattccatg acagccagaa tcacctgctc tccctgcgcc atgagctgtt
    14581 gggcgatctc ataggccgcc agcccgcccg tgcaagttcc accgatgagg tacggtccat
    14641 gcggttgaat cgaccgtatt tcctcgatgt aatgcgcggc catctcctcc acgcgcatga
    14701 acggtttttc ttttccgtcg agtccgcggg cctgcagtcc ataaaacggt tgggcatccc
    14761 cgagcaactt ggccaggcgg gcaaatacga gcacattgcc gccgaccccc ggaaccgcaa
    14821 aaaagggagg atttttcccc tccggttgaa tcgcgaccaa cgaccgccag cgcacggtgc
    14881 acccttcgcc ggccaggaca tccgccaatt gttcgatcgt gggcgcctga aacaataccg
    14941 ccatcggcag gcggatgctg aacgtctgct cgatggcgct gaacatccgg agcgccagca
    15001 gggaatagcc gcccagcgcg aagaagttgt cgcgcacgcc gatgggtgtg attcccagga
    15061 cctgctccca gatggccacc aactgcaatt caactcgatt tctgggctcg atcggctggg
    15121 cgctgtcatg caccggctcc gtcaccggcg ctggcaaggc gccacgatcc acctttccat
    15181 tcggcgtaag aggaaattcg ttgagcggca caatcgccgc cggcaccata taatccggca
    15241 ccatgtctcg cagggcccgt cgaagcgcct gcggatcgca acggcgccct tctctcgcca
    15301 ccacataggc caccagccgc ttgtctcccg gtgcatcttc ccgcacgatg accaccgcct
    15361 gcttcaccgt cggatcgtcc gccaggaccg cctcgatttc tccgagttcg atccggaacc
    15421 cgcgtagttt gacctgataa tcgatacgcc cgatatactc caatcgtccg tcaggcagcc
    15481 atttgacctg gtcacccgtg cggtagagcc gctcgcccgc ccggaacgga ctggagacga
    15541 atcgctccgc cgtcagctga ggagcgcccc ggtatccgcg cgcaagcccc atcccgccga
    15601 tatacagttc acccgggatt cccaccggga ccggttcccg gttcagatcc aacacataga
    15661 cctgagtatt cgcaatcggc cgcccgagag aaatcgtgcg ttcagcagta cgcacgcgct
    15721 ccaacgtcga ccaaatcgtc gtctccgtcg gtccatacac attccagacc gatcccgctc
    15781 gggcaagcag ctcttgggct aattcccgtg gcaagccttc cccgccgcac agcactttga
    15841 cctgtcgccc cccctgccat cctgactgca acaccatccg ccaggtggca ggcgtcgcct
    15901 gcatcatggt cacagcgccc tgatcaagtt cgcgttgcag ccacgctccc tccatcgcct
    15961 gaagacgacc ggccagaata atgcgggccc cgaccagcag cggaagatag agctccaaac
    16021 cggcgatatc gaacgacagc ggcgtgatgg cgagcaggac gtcccgactg ctgatgcccg
    16081 gctcctgttg catggagcgg agaaaattca ccagggcacc atgttcgatc tcgacgccct
    16141 tcggctgacc ggtcgatcct gaggtgaaca ggacatagac gagatgctcg ggtttcgcga
    16201 ggggtgcagg atccttctcc ggataccgcg tcagtgactc cgcctccccg tccaggcaga
    16261 ccacccgaag ctggtgctgg ggaagcccgc caaggagcgt cgagtctgtc acgagcaccg
    16321 ccgcgtgact cgcttcgatc atggccgcca acctgcggat cggtaatcca ggaaccaacg
    16381 gcaggtacgc gccaccggcc ttcatgaccg ccaggagact gatgagcaat tcgggagatc
    16441 gttcgaggaa caccggcacc accacatccg ggcccacgcc ctgttcccgc aagcgatgcg
    16501 ccagccgatt cgcgcgccga ttcagttccc catacgacaa ttcagcgcct tcacacacca
    16561 cggcgatcgc atccggagtc cggccgacct gcgcttcaaa tagttgcggg aaacaggtct
    16621 ctcgctccac cggcctgtcg gtcgcgttcc attcatgaag gatccgatgt tgttcctgtt
    16681 ccgtgaggat cgccacacgt ccgaccttct cttccggatg ctccgcgatg acctccatga
    16741 attgacggta ctccgccagc caacgttcgg cggaggtgcg atcgaacagg tccgtgttga
    16801 actccagata ggcttttcgc gaagccaacg ggtcgatcga caaaccgaga tcgagctgtg
    16861 ccgcaccccg actgacttca taaggggtcc acgacagatg ctgaaactcc gtccgggcga
    16921 agggggtatt ggcgaagttg aacaggactt gtaccagggg caacccgccc tggctgcgat
    16981 ccgggcgtag ctcttccacg agcttttcaa acggaatgtc ctggtgcgca taggcatcga
    17041 gggacagatc tctcaccttc tgcagtaatt cgcggaaggt cagttccccc gtgacatccg
    17101 tccgcaacac cagcgtattc acgaacgtgc cgactaaatc ctcgatctcc agccgattac
    17161 ggttcgcgat cggcgtcccg atcaccagat cacgctgctg cgtgaggcga tgcaggaggc
    17221 cgaagaatcc ggccaggaac accatgtaca acgtgacgcc ttcgcgcacg ctgagctgct
    17281 tgaggcgatt cacgagcgcc caggacaggt cgagagagac ctgatcgccc ttgaacgaat
    17341 ggatgggcaa ccgcggccga tcggccgaca aggcgagcac cggcagatcc gtgagtttgg
    17401 atttccagtg cgcgagttgc tcagccaaca ccgaaccggt cagccatgtc cgttgccact
    17461 gggcgaaatc ggcatactgg atcgcgaggt ccggttgaat cgcgacgggt ttccccgcac
    17521 agaacgcgtt gtagcacttc accagttcac gggcgatcac gccgtacgac cattgatcgg
    17581 agatgatgtg gtgcgtgctg accaccaaca catgatcctc ttcgcccaat tgaaccagca
    17641 aaatacgcag caacggaccc tgcccgaggt cgaaaggacg gcgcgcctcg gcggtcgcca
    17701 gttctaacgc ccgagcctcg cgcatctcac ggggcaaccc gcgcagatct tcttccaccc
    17761 agggcggatc caacgaggta tgaacaatct ggcgcgcctg accatccacc gtggcaaagg
    17821 tcgtccggag tgcgtcgtgg cggcgcacca gctccgccac accccaacgc aacgccgccc
    17881 gattgagcgg accgtgcaag cgcacgctcg ccggaatgtt gtaggcggcg ccggtcggcg
    17941 acaactgata gagaaaccac atccgttcct gcgcaaacga cagcggcaag ggcgctcctc
    18001 ccgccgcttt ggtcatcatt cgtccgtccc gggccgcctg ctgcgttacg ccctcctgag
    18061 atcgcttgat cacctcagcc agtgtcgcga tggtcggccg ttcgaacact tgtcgcagag
    18121 gcaggacgac ccggaagagg gcttggactc tggacaccag ctgtgtcgcc agcagcgaat
    18181 ggccccccag ttcgaagaag ttgtcatgca cgccgacatc cttcacatgg aggatttccc
    18241 cccagatgtc ggccagcact tgctctgtgg gcgtccgcgg ggcgagaaac tcagacgtga
    18301 gatcgacctt gctcgcgcga ctgtccgtag cgacacggag cgcagcgcga tccacctttc
    18361 cattgacggt caggggaagc gtctccatct cgacgaaggc ggaaggaatc atgtgcgcgg
    18421 gcaactggct ccgcagctgt tcgcgtagct gcaccagtcc cagccggttc tgcggacgag
    18481 ggaccatgaa ggcgacgatc cgcttatcac ccggctgatc ttcccgcacc tcaaccaccg
    18541 cgcgctcgag gtccgggtgt cgattcagca ccgcctcgat ctcttccagt tcgatccgat
    18601 agccgcgaat cttgacctgg cgatccacac ggccacgata gtcgagctgc ccgtccggtc
    18661 gccagcgcac cagatccccg gtccgataca gacgggccct cggacggctt gagaacggat
    18721 cgggaataaa cttcgtgtca gtgagctccg gtcggccacg atagcctcgc gccaccccca
    18781 caccaccgac atagagttct ccgggcacgc ctaccggaac cggacggcga tgccgatcca
    18841 acacatagac ggaagtctga gcaatcacgc gcccgatcgg cacgtcctcg cgcaactcct
    18901 cctgcagccc gaggcccgtg agatccgacc aggtgacggc gaccgtcgtt tccgtcggcc
    18961 cgtaggtgtt caacaatcga accgtccgac cgaccagttt cgcccaccgc tgcacgatct
    19021 ggggaaggac acgttctcca ccgatgatca ccgtcttcac cgactccggg aaggccagtt
    19081 gctccaactc cattcgagtg acgacttcat gccagtaggc cgtcggcaag tccaacagtg
    19141 tcagctgcca gtcgcggcat cgatccaaga aacccgacac cgaatcgatc atcatctgag
    19201 tgcgcagcac caacgtcgcg cccgtcgtga ggcagggaaa aatttcttcg gcggcggtgt
    19261 cgaaactcat cgacgcgaat tgcagcaccc ggtcgcgcga gtggacaccc gcgatctccg
    19321 tgatcgcccg cacatagttg gcgatcgatc ggtgctcgat catcaccccc ttgggctggc
    19381 cggtcgaacc ggaggtgtaa atcacataag ccagattctc cggcgcgtcc gtgccttcga
    19441 tggcatcgtc cgggaaacgg gcgatttggt cccattcgga atcgagacaa atcgtgtggg
    19501 ggttcgtcgc aggcagccgg gcgagttgat cctgttgcgt caccaacacc cggacctggc
    19561 tatctctcag catgtattcc aagcggtcgg tcggataatc ggcatccagg ggcaggtagg
    19621 ccccgcccga tttgagaatg ccgagcatac cgacgatcaa gttgagcgat cgttcaaggc
    19681 agaggccgac aaccacaccg ggtgcgacac ccctccggcg gaggtagtgg gccacacggt
    19741 tcgcgcgcgc attcaactcc cgataggtca attcctggtc gccctgtcga accgcgatcg
    19801 catcaggggt gcgcaacacc tgtgcttcga tctggcgatg gaggcactgt gccggcgcct
    19861 gcagttccgg agactgtccc cacgtcagga ctgtctcgcg acactccgcc tcggacatga
    19921 gaggcagtga gccgatctgc acggcggggt cggcgacaag cccctccagc acgcggacaa
    19981 aatgctgctg catccgtgcc atccggtcgg gcgtaaagag gtcgtctttg tattcgaagt
    20041 agccggttac cccctgctcc gattcggcga gctcaaggca gagatcaaac tgcccgctct
    20101 gctggggaat cacataggcc tcccacggag gcaggcacga ttcagaaaca gatgacgacg
    20161 acccactcat ccggccatcg agttccgaga gcaaccggaa ctgttgcaac acgaacagga
    20221 tttgccccaa cggggcgcgt tgacgatcac gggccggtcg caagtccgac acgagccgcg
    20281 catagggata gtcctgatga tcgagggctt ccagcagagt acgcttagtc agcgccagca
    20341 attcgcggcc ggtcgaatcg acctgaaccg tctcacgcaa cacgagcata ttcacaaaat
    20401 caccgacggt ccgggcgaat cgagaccggc ttcgaccgaa taccggagtg accacggctg
    20461 tgtcttccac attcgtgtag cgatacagca gggcgtgaag cgcagtcaga cagacggcat
    20521 acagtgtcgt gccttccgcc tgcgcgaatg ccttgagccg ctcggtcaat gcccgatcga
    20581 tatggatcgt ctgccacgca tatcggcttg gctcaaccgt cggctgcggc cgatcgtaga
    20641 gcatgtcata gttcgggagt tcacctgcca gtcgcgcctg ccaatactga gccagccgac
    20701 cgccctcagc actctccagc aacgcgcgat gccaaccgat gaacgcaccg tagggggccg
    20761 gctggctgcc gagtccgttc gatccatcct gaggcaggga tccatcggag gcgtagtcct
    20821 gcttgaggtc ctcgaccagg tggatcatcg accaaccgtc cacggcgata tgatgcgcga
    20881 ccagcagcaa ccacgcctgt tgtttcccct tgaataacga cgccctccac aaagggccgt
    20941 gcgccagatt gaacggaatc gccgccgctt ccatggcttc ccgccgcaag cgcgcccaat
    21001 cccatctgga ggcatcaatg accgtccaga tcaaaggcaa ccgggcatga attcgttgaa
    21061 ccggcacctc gtcctgcgtt tcataggtcg tccgaagcgt cgcatggcgc tcaccgagtc
    21121 gatcaagcgc ctgtttcagc gcggcctgat tcacgtcgaa cggcagaggc agaatgacgg
    21181 agacgttggc ggcggcgcta tccggagcca actggctgag gaaccacaga gcagcctgat
    21241 tctcggacag tggggcgaca ttctccgttg tgagattctg gccaccggca gcaatgagtt
    21301 cactgccttc ccggagcatt cccgcttcga tggtcgcagc cagatcgccg agtgtggcac
    21361 ctcccaggag atgctgcaac gaaagcggaa cctggaatga ctcttccacg cgatgcgaga
    21421 cctcagcggc catcaacgaa tcgagcccca ggagatgtat cggcccgtca tgaacgatgg
    21481 tctctcggct gcacccgagg cgatcggcaa tcatcccctg cacagcctgc tcaatctgcc
    21541 gccgccgcgc atcggacgag agcgcgcgca gatcttccag gttcacgacg gttgtttgcg
    21601 cctgtgacag gggtgccggc aagacactct tcccgatgat ggaaagttgg ccggtcagaa
    21661 attgatcgcg gcaggcccgt cgctggagtt ttccactcga cgtcttcggc agggtcccgg
    21721 ccttgatgaa gatgatggag aagacatgga ggtcatgctg ttcggagaca gcagaacgaa
    21781 tcgcggcagc cagctcatcg atggccaagg ccgttgcctg acgctcgacc tcctgcacaa
    21841 ccactaccac ttcctccccc gcatcctgga cggagaaagc cgccgctccc ccgccccgaa
    21901 acagcctgtg gctttgctct acggttcgct caatatcctg cgggtagtga tttctgcctc
    21961 gaacgatgag caggtccttc aaacgcccgg tgacaaagac ttcaccgtct ctgacgaacc
    22021 cgaggtcacc ggtccgcagg aacggccctt ctcccgtatc gcgaagtgtc gcgccaaacg
    22081 tgcgcgcagt ttcctccgga ttgttccaat atccctgggt ggtgctggct ccggctaacc
    22141 agatttcacc gacctgttgc acggcacagc gggacagggt ttccggatgg gcgatgacca
    22201 cccgggtatc acccaccgga gcaccgcatc caacgacctg caggacggga gcgataccgg
    22261 ccttggcttc cgacaccacc ccccgttcca acgtcgccgg atccagggac agcaccgtcg
    22321 gtgcgacacc ttcccgcttc aaggagatca acaaggtgta ttccgccatc ccgtaggcgg
    22381 ggaaaaacgc ttcacgccgg aatccggccg gcgcaaacgc ttcggcaaat cggtcaatcg
    22441 tatcgtgggc gatcggttca gcgccgcaac tcgcaacctg ccaaccggag agatccagcc
    22501 ttgcgcgatc ttcggctgcg atggtttcca cacagcgacg gtacgcaaag ttgggcgccc
    22561 cactgtgcgt gacgtgatac cgctgaatcg cttcgagcca gcgtaacggc cgcttcagaa
    22621 acgtgagcgg cgacatgagg taggccgggc ggccgatcca agcaggctgg acgattccct
    22681 tcaccaatcc atagtcatgg aaatgcggca tccaagacag ggtgacggaa tcggcatcgt
    22741 aacagccggc ctcggtgatg caccggctct gtgcggtcat attcccgtga ctgaccatca
    22801 cacctttggg ggcggaggtc gagccggacg tatattgcaa gtaagccaga gtggaaggga
    22861 gcggatggat cgacctccac tgagccgtcg agtccggcct ggacgcatca caaatcaccc
    22921 actgttcgag cggaatcgtg gttccttgcg cccgaaccag ctccagaatc tggttggtgc
    22981 tcaaggctcc ggccgcgccg gcatcctcgg caatacgctg gacgcgcgtg acaccgaccc
    23041 ttgcccgaaa ggcatccggc ggggggaccg gcacggcgac taacccggca tacaacgccc
    23101 cccaaaaggc ttggacgaat tcaagaccgg gcggatacac caggagcaag cggtctcccg
    23161 ctgcgtacct ggattgcaga tgaccggcaa tcgagcgagc tttggcatcg agctgtctgt
    23221 acgtcaggac ggtatcgggg ctgatcccat cgcgcaggaa gatgtacgcc gactggtcgg
    23281 gttgatgctc agcccgccaa cgtaggacat caagaagagt ctcgccgggt ggaatgctaa
    23341 gggatggatg tctgaccaca gaatgctcta ccccagtggg accgagaagc ggacaagaac
    23401 tatgcttcgt agctatatcg gatggatgac tggaaaagat cagtcacgaa ctgtctccaa
    23461 ccggtctcac cacgccaggt gagtctacgg tggaaccgat tcatcgcaaa acggaatcac
    23521 gttttcctca cgtcgtgact tccccctaag gatgaaagaa atattctcca agcacatgat
    23581 ttcatttgaa agttacgctc gaccacgcta agctgcacat ctttgccggc ctggtcacgg
    23641 agaggtgcga gagcggccga atcggacggt ctcgaaaacc gttgtcctga aagggaccgt
    23701 gggttcgaat cccaccctct ccgccacacc tgcggcaacg cctcaaaact ctgccggtgg
    23761 gattcgaacg ggggttctga agggggcata cccccttcac ggggtgaccg gagcgaagcg
    23821 gaggcgcgag ggggcctggc cccttagcgg gagcgtgtgc gaagcaccta agcgcgactt
    23881 cgaattccca ccctctccgc catacaaagc ttgcttgctc cccgagccca ggtaatggtc
    23941 ttggcgacgg gataatcacc aacagtgccc cacctcccct gtcgactgct cgaccggagc
    24001 cgtccgttgc tcaatagccc ctcggtttcc cccaactccc cgtagttcct ttgggtttca
    24061 gcgacgtgtc ggcccagcac ctaccgaagt tttgggcgct ccccctcctc cagtggtctg
    24121 ttcgtcacca cagttccagg ctactctctg tttcacacag gagggccgca tgtttgaaca
    24181 aactgaagct gcgctggtga tgacgctgtt ggccttgggg ggaatcgcat tgatggcgga
    24241 tgagtcgatt ggaacgacgt gcatgcagat ggtgaaatgg cttcgaacca gaggcagacg
    24301 ttaaccggct cttttcttca actccttccg caattcgacc atggccaggg tgtcgcgttc
    24361 gcagtaccgc agtaacgcct cttcgatctg cgccttttcg atccagtcac tgaccgtaaa
    24421 gatcatacgg gcatattcgc aggctgccac gcccccgtca cggatagcca gatccgcata
    24481 actcaacgag ggcaccacag ccggcaggac ggccttgatc gaatagctcc cttcgaaggc
    24541 cggatggtag tagtgatccc tgatgacaat gtgaaggtcc cacagtctcg cgatcacccg
    24601 cttcaggtcc tttcgcagtg acggaaaatc ttcagccaga cgctccaaca ccgaacgttc
    24661 ataactcgaa tacacgcaga tactgccttc cccacccaac gaatccagca acgtgacggc
    24721 caactcttca cgcggatcac gcccgtcgcg gcagaggtac tcggcgtggt cgagcctccc
    24781 ctcagggtgt tcgatgtggt tggaccattg agtgggaatc acctgatagg gcctagtatc
    24841 cccgaatttc ggcacggccg gcatgaaggt ctcgaaatcc aaatgatgca cgggatacac
    24901 gatcttctcc agcgccgacc gcaagccttc cccgatccat tcccgattgt ccctcacccg
    24961 tcgctgtacc tgcgtcaacg tcacgtgatc gggaatttcg tcgatggtct ccacccccaa
    25021 ctcgcgcagc tgaatgaccg tcttgctgct cccgggcaga tgataaatcc agcgaggcgg
    25081 cttctcctgc gtgcaatgcg cccagaaggg acattcgtag gggctgtgac aatgttcatc
    25141 cggggcaatg ggcggcgccg acatctcgct caacgcggcg tgcatcgacg tcagacgtga
    25201 gggcacatcc ggcaaccgtg ggcgtatcgt ttcatttaag gagcggagac taaacaattg
    25261 gccaaggtcg agttccacgc cgtcgaacac atactgggtg ttcacgtgca tgaggcagat
    25321 gtcgtcgaga accaagccgg ccccttcgag gacgtaactc tggatcgtca aatcctccac
    25381 atggatcggc ttcaacttgg tagaggattt cacttccacc agccgccagg tgggttgccc
    25441 tgactcgttc accccgacac gctccaggac atcgacacgg atcaagacct ggtcgaactg
    25501 aaatgcgcct tcgaagatgg ccgacaccgt cgaatcctgg agaagctccg ccgtctgcgc
    25561 cagggcctcc tgagattggc ggtacccggc cgtgacgagg cgaccgccgg gaaatcgccg
    25621 acgggcgagt tcgccgagat ccgtgcccat gtcgagaatt gcttgggtgg ccgcatccgg
    25681 cttcgtggcc agcgccggag catgaatttc caggtacaga cgcttatggc actgcaggcc
    25741 cgacagatat cgcgattttg acagccgata ggaggaacga gcggcaggct cgggcgtgat
    25801 caacatggat aggttcaggc taccgaagcc gtgcgcctct tgtccagtcc caggagaagg
    25861 acatcctcgt ccgcaaccga gttctggtag gatgacgccc catggcaaac ggcgtcgcac
    25921 aaatcagacg atcggtcatg accctcgcgc tcccggtgac ggtgagcagc ctgctgcagc
    25981 gaaccgaagg catcgtcgcg gtctttctcg tcggaggatt gggcgcaatc cccattgccg
    26041 ctgtggggtt gggccaactg cttgcgttta tcgcaacgac actcgtgtcg ggtctctcgg
    26101 tcgggaccaa cgtcatcatc gctcaacagt ggggtgcccg ccggtatgaa gaagcaggcc
    26161 aggcctcccg gcattttcta ggcctgtcga tattcgtgtc cttcgccctg gccctgctcg
    26221 gcctttcggc gaacggactc atcatgcagc tgctcggagc gcagccggag gtcatcggcc
    26281 tcgcactccc ctactcaaac ctgatctttc tggtcatccc cttcaccgtg cttctggcag
    26341 tactgtcatc gattctccag gggaccgggg acaccaagac accgatgtac gccatgatca
    26401 tggtcaatgt gctccacatc gccattgcct atccgctgat ctatggccaa tggggattgc
    26461 cggcgttcgg cgtgaagggc gcggccgtcg ccgttgggat tgcggaggcg accggatcag
    26521 tctatctgtt gttgcgctgc cgggcgatcc tcaaaccgtc gaagcggctc aggcgagacc
    26581 tgctccgcac catctggcaa gtgggtgctc cggtctccgg agaacggatc gtgcaacagg
    26641 ccggaattct gctctacacc aaaatcgtat tgatctacgg cacggtctcc tacgccgcgc
    26701 atcaagtcgg actctcaatt gaatcgctct cgttcctgcc tggctacggc tttgccatcg
    26761 ccgccgccac catggtcggg caaagtatcg gcgccggcaa atacaccagg gccaaactcg
    26821 aaaactggga agcgaaccgg ctggcgacgt tcatcatgtc cgccatgggc atcgtgtttt
    26881 tcttttttcc ctatgccctg ctgcgcgcgt tcaccagcga tgaagcggtg atcgagctgg
    26941 gaacgacttt cttgaagatc gtcgcgcttc tgcaggtgcc gttggccctg accatggtgc
    27001 tggccggctc actgcgcggc gcgggcgata cgcgattcat catgatcgcg acgatgatcg
    27061 gcatgtgggg cgtgcgtatc cccatcgcgt tcgtggcagg ctactggctg acaatgggag
    27121 tgttttacgt ctggctggcc atgatcgcgg actggacgct acgaatggcg ctcatgctct
    27181 ggcgctatcg atcggagcgg tggaaatcga tccgagtgct ccgttcctcg acgacgtgaa
    27241 ccgcttagcg tgagccctca gtcgaacact ccggaggcct gacagacggc cgcgtatcct
    27301 tccccgatcc ggcccgtacc tcgatgcgtc ccaccgcctt cacgttggcg cagaggtcac
    27361 ccaggcccgg cgtcaccagg cggtagggac cacccttgcc gtccggtagc ggctggccgt
    27421 cgagttcgta gagcaacaac ccgaaatccc gcgcctgttg caaggtgagc gtcgccgcgt
    27481 acttgccatc cacggaatgg aatgtgacat ggtcggcatc gaccgcaagg gctggaatat
    27541 ccaacaggcc attgacgcga atggcccggc cgcgcatcga cggcatcagc tgactgatat
    27601 cttcgacatg ttgtgcttgc ggcagttgcg ccagcgccgc tcgcgtaaga gaaaccggtt
    27661 ggacgaccgc gccatctatc cggacgacac cgggaccagc gggctcccgc tccgtgatgg
    27721 ctttcaccat cgccgttaac gcctcgttca ccggtgtggg aatccccaac tcccgcccca
    27781 tacgaacaat gtaaccgttc agatagtcga tctccgtcgg gcgtttcgct ttccagtcat
    27841 cgtacatcga ggtatggatg tcacggagtt cctgtgtcca cttgacgacc ttctcggcca
    27901 tatccggcgc cagaggcacc ttcaccgccg ccgacaccgc catcacttcg cccacgatct
    27961 gccggatcac accggccatc tccggatggt ccagcgcctt ggccaccttg tcgtcgatca
    28021 agaccgtgag ggggttaaag acgcagttcc agcacatctt ttcccatttg ctcttcctga
    28081 tatcgtcgct gagctggcag gaaatgccag cctgcttgaa gatctccgcg atctgcaata
    28141 cccgttcgct cttgtgaccc atgagttcgc cgatggccac gccgccccgc ttgtaatgtt
    28201 caatgacgcc gggcgccacg atcttcgagt agatgaacgc tacgccgccg accacagagt
    28261 cgcgatgcac ccgcgcgatg atgcggtctt cggtgtcgat gccgttttga agcgtgagca
    28321 tgaccgtacg ctcggtgagc accggctcca actgcgccat gacttcatcg aggtcgtagg
    28381 ccttcaccgc cagaatgatg agatccggtg gcgccagctc tcgcggatcg gaagccgccg
    28441 gtggatgcac ggtgaagttg cccttggcac ttttgatcat caagccatgg cgcttcacgg
    28501 cctccagggt gcggggtcgc aggagaaacg agacgttcgg attatttttt gcgagatggg
    28561 ccccgaagaa accgccgacg gaaccagccc caaccaccat gatctgcttc atgagactcc
    28621 tcactcgttg cgttcgaaag gacggtacta tagcatgcag ccgcgcacct gaaacagtga
    28681 tcgctccctc gcgttgagac ctatgggaac cggatcaact ctcggtctga ttcgacagaa
    28741 gctggcctcg gcacgatcgg tgaccgtgct caccggcgcc gggatttccg ccgacagcgg
    28801 cgtgccgacc tttcgcgggg tcgatggact ctggcggaac tttcgcgcgg aggatctcgc
    28861 cacacccgag gccttcgcgc gtgatccccg cctcgtgtgg gaatggtaca actggcggcg
    28921 tgaactcatc gcctccaaac gacccaaccc cgcgcacgat gccgtggctc agatggagca
    28981 acgcttcgag cggttttggc tcatcacgca gaatgtggac ggactgcacc gcgacgccgg
    29041 ctctcgcagg ttatccgaga ttcacggcaa catctggatg gtgcgctgca cgcagtgccg
    29101 gcgcgtaact gaaaatcgcg acgtgccgat tgcaatcctg cccctctgca gcaactgcaa
    29161 cgggctgctc cgcccgcata tcgtctggtt tggagaagca ctcgccgagc aggatcttga
    29221 cgccagcgag gccgccctgc agtccagcga cctgtgcctg attatcggca cctccggcct
    29281 ggtgtatcct gcggctggtt ttggttcgat cgcgaaacaa gcaggcgcgt ttgtcgtaga
    29341 aatcaatctc gatgccaccg cacagtcagg catcgtcgat gctgcgcttc aagggcgcgc
    29401 caaggacatc gttcccttgt tattgcaggc gtgagagcag cggcaacaat tcgttgaggg
    29461 taccgattgt cgtcccgccc gaaggacgcg ccccctcccc tctcgcgagc aacaccccgg
    29521 tc
//
LOCUS       DAORRQ010000053        28529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_103_length_28529_cov_48.188874, whole genome shotgun sequence.
ACCESSION   DAORRQ010000053 DAORRQ010000000
VERSION     DAORRQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28529
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103_length_28529_cov_48.188874"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="PK224_15215"
     CDS             complement(<1..1436)
                     /locus_tag="PK224_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB17144.1"
                     /translation="MFGTITTLCTPRDASPRPGTCITIHVLLCSLLLMLANHGSFVHA
                     QEPSSTVSRSFAIAPQPLSSALLQFSDATQIEVLFDATIARDLQTQGVSGDYAPEEAL
                     RMLLQDTGLSYRTTSAGSVTLEQAAGLPIVPAPTVQSPPTEPTPQAVSRPAAQKSVKV
                     PEIVVKDVRERDDDAQTYIAEESTTATRTDTPIRDVPQSIQVITRKVIEEQRTFRLQN
                     TLENVSGINATESAASLYDSLIIRGFSATDHSYFRNGLLDPFAQFTASDTYNIRRLEV
                     LKGPAAVLYGQGDPGGVINIVTNKPLPNAGYSANVTLGNFNFYRSELDATGPLNADKT
                     VLYRLNVAGQKAGSFIDYANRDLAAIAPSITWLMSSRTTLTVEADYLRRWSNDPYGLP
                     AQGTILPNFNGPIPRNRSVTLGDFSTFNRTSYRFGYDLTHQFNDKWSIRNAFRHTIVE
                     DDKNNLYAGGFFLEPDERTFQRFQVLQPA"
     gene            complement(1542..2531)
                     /locus_tag="PK224_15220"
     CDS             complement(1542..2531)
                     /locus_tag="PK224_15220"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRB17145.1"
                     /translation="MDTPERDRLASTASEWLVRMESGTITATERLRFAEWLSSDPSHR
                     DAYREAERFWHGLDRLNPDDIREFDRYLTEDTITDPRGTSRSWRRVAALAASLVLVAG
                     IGLWITTAWLPFGDYRTAVGEERTVTLADGSTVQLNTDTALSVSMTEQGRRLTLHRGE
                     AFFTVAPDAARPFEVEAGRGTIRALGTAFNVRTGSDRTTVTVTEHSIRIRLGKETSAE
                     VHAGERLRYRPDGWIDPVETADMDRVLAWRHHRLIFENQPLPEVLAELARYRSGRLVI
                     LRDQSLKTLPVTGSFDTERLDHLLPAIEESLPVRIVSFADRVILLYRGLPKKS"
     gene            complement(2643..3161)
                     /locus_tag="PK224_15225"
     CDS             complement(2643..3161)
                     /locus_tag="PK224_15225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB17146.1"
                     /translation="MEARTHLEMSTLIREHGHDLQQFLTRRLGCTETAKDLVQDTFLR
                     LLQNRSGEVLTNPRAFLFRIATNLVIDHHRRRQYRETVTLDDPERPIDQADPAPSIET
                     VVWSKQQVARLKQAIEELPPKCRQVFVLIKFHHLSHAEVAVKLGISQSTVVKHMVKAV
                     DFCRSRLQDLSS"
     gene            3381..3875
                     /locus_tag="PK224_15230"
     CDS             3381..3875
                     /locus_tag="PK224_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRB17147.1"
                     /translation="MKSMRPDQVSGKRASASEQAIDEIRSSLGCSGLRWSPQREQIVM
                     AFLKQDHISIRSLYGLLNRNGRRAPLNTIYRTMRVLCDNGFAQARRFGEETQYDNISA
                     KRDHDHLICTGCGYIVEFEEPAIEGLRHQVASANGFHLTGRNLELYGLCAVCRVDEHP
                     VVRT"
     gene            complement(3910..5136)
                     /locus_tag="PK224_15235"
     CDS             complement(3910..5136)
                     /locus_tag="PK224_15235"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRB17148.1"
                     /translation="MIVSQLQPVRVPSNTSRPQTSAPPAVAALPQPARKVWRKVWLNL
                     HLYVGLLGGALFVLTSLTGSLLVFYKTIDEWMNPEQLIRTAGSDQSLSDIVAGARTVH
                     PDWSPPDTLIFPLHERDTFHAWFKDPAATPPEERWHVVAVDPSTARPLSDRQWGSFFV
                     SFIYELHQELLLGKPGEVFVGIMAVFLLVSIGTGLYLWWPALGKLRRALSFQSGGSPI
                     RRHYDVHKLTGLAGTLLLTLLAVTGFYLEFPDAVTSVVRWFSPVRDTSPELQPQSEPQ
                     TGLPKLPPDQAIAIARTVFPDAKPMWLGLPQHDRDSYSVGLRQPGEVRQAGGQTEVWI
                     DQYSGAVRRVQDWRQFTRGETLLSWLFPLHNGEAFGLTGRWIIFFAGLTPLLLYLTAL
                     RMWWLKRVAHQRRRDP"
     gene            complement(5713..8289)
                     /locus_tag="PK224_15240"
     CDS             complement(5713..8289)
                     /locus_tag="PK224_15240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB17149.1"
                     /translation="MMHLSWQVVVTLSVSAVLVHTAGNLAEARSERLTTSNNSPLLLA
                     QATAAAETSDTVDFNIPPQALGSAITAFGDQANYRLLLPSDLAEGRSTSGVSGRHTPQ
                     EALALMLAGTGLSYRMTEARTMTLEPVAATPLPLAPLSQEPAPPNPNTQIQAVPKPVK
                     VPEIVVKEVEDRGYAVDESSSATRIPAPIHDTPRSVEVVTRQVLDDQKVIRFSEALRN
                     VSGVSQSSTQGGQGGTFMIRGFASDLNVFKNGFRDDSTFSARAQRDIINIESVEVVKG
                     PPSYLYGRSDPGGVINQVTKAPLRNPYYSAEMIVGSYGLYRPTIDIGGPLNESKTLTY
                     RFNGMYESAESYRDGVKTERIFLAPTFGWEMSSRTTLRFEAEYMYDRAPIDRGLVAIG
                     NGVAPIPVSRFLGDPSRKLETHHGKATITLWHDISNMFRWRTAFRTAVTSSRYSSLES
                     NFLVGAESDGILNLARYEIPTTVQSHYLQNELHGNFTTGSIKHKTIIGIELGRENSSA
                     TASGDFGGDTSTPGAFSYINIFNTNDRLFLNPALTKFSDASTQNNILGAYFGDQVDLL
                     DNLHVHFGGRFDLFDQTITNHPDDFTATSSQNNKTDSAFSPSVGVAYQPWKPITLFAN
                     YTESFAPQSAGSRSINGNLFNPERGKSYEGGIKYQAFGGRLRSTVALFDTRKKNVLTA
                     DPLNGFFFSVATGEQRSKGVEFDIAGQILPGWDIIANYAYIDARVTKDLLFAEGSRAP
                     NSALHQGSLWTTYFFQEGVVQGFGAGIGMYAQGKRNGIFQCQDPANCQAPFEMAGYVR
                     MDAALYYRKQEVFNKTNLLAAINFTNVLDHRYFSGAQNFREIVYTGAPFTAIGSLKFE
                     FH"
     gene            complement(8501..9490)
                     /locus_tag="PK224_15245"
     CDS             complement(8501..9490)
                     /locus_tag="PK224_15245"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRB17150.1"
                     /translation="MPDSTPPAASSPLIDEALAWLVRIHSGHASDEERLACTAWRTQS
                     SAHQLAYTEAEILWRDIGLLPNGDCTGSADLTASMPHGTLPHARRTIKWGLAACVLLA
                     AGWLSWGIVQERLDLASADYRTAVGEQRHVKLEDGSRIHLNTDTAVAVAFTPQQRTIR
                     LLTGEASFTVTPETVRPFTVQSGDVSTRALGTSFLVQRHQNAITVTVTEHAVQVSATD
                     GRQVPAAVVRQSEQISYSAEAGLGRAHAVDSNAIAAWQRGKMIFEAQPLAAVVEELNR
                     YRAGRIMILNPALRSLNVTGVFDIGDPDAALRMIEQTLRIHDTTLTPYLVLLH"
     gene            complement(9583..10110)
                     /locus_tag="PK224_15250"
     CDS             complement(9583..10110)
                     /locus_tag="PK224_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB17151.1"
                     /translation="MQNEHTLPCLTAFEEHHNDLLRFFTHKLGCRDLAADCTQDTYMH
                     LVRMRPSIDVQNPRAFLFRVAANLAVDNLRKLRTRRGALSIEPPPEETASPAPSAEDT
                     LDAKQRVAQLEVAMRELSPKCRRALLLNRLEGKTHREIADSLKVSESMVAKYIVQALR
                     HCRARLHPEESTRSS"
     gene            complement(10119..11042)
                     /locus_tag="PK224_15255"
     CDS             complement(10119..11042)
                     /locus_tag="PK224_15255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRB17152.1"
                     /translation="MNDKRESLARMGGWLVSLTLHGLSLGTAFVLAAEFSILPEPRPF
                     RWEVTMVSAPALQPVAADVPTPFSASSVSPSVMPRDKSPRQVRSRPNAANTEAARPLE
                     THSGIIAPAQDGFVRPLATENPSLVVDSVSPGPHTPSADLTSDELVHEAPQVTRVDLP
                     VTTTEPSEIPPPSVDAAEVSHTTSMPSIRESIRLVNRPTPRAREAIVSRILHADYGWL
                     AEMLFAKVEQSKRYPTFAKSRRWQGNVMLQAVIHEDGSISDITIVTPSGHSILDLDAV
                     ALLEQASPVQLKYPLGQSHVVVHIPIGYRLE"
     gene            complement(11039..11542)
                     /locus_tag="PK224_15260"
     CDS             complement(11039..11542)
                     /locus_tag="PK224_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRB17153.1"
                     /translation="MHQDRRARTIDTSIIGIVSSGSPPLSIEASRQRPLHSLRTKPQP
                     FWPTPQITPLNSVTPAARLSALLSKRSGDRLTLKELSTLLGYSEKYTSEIFRKYMGLS
                     FSEYLKQLHLAKATKLLSDQSLTIAQIAESVGFSDAFAFSHFFKRAIGCSPSEFRKQQ
                     TPEAGLR"
     gene            complement(11762..12514)
                     /locus_tag="PK224_15265"
     CDS             complement(11762..12514)
                     /locus_tag="PK224_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRB17154.1"
                     /translation="MLDLVGLGILAGVVPVYLGIAAALVLRRALPRTWEAGLIGLSTG
                     VLTYLFFDLMHEAVELMGVRDPFSWLLFLGSLFLSFVGLVHLEKHQFLKGRHADQALT
                     LPFVIAAGMGLHNLGEGLAIGTSYAGGEWALSGLLVAGFALHNGTEGFGIVGAAGPSP
                     IPPRTVVLLGLTGGAPTCIGTVLSGLTVSPYLSLVWYALAAGSLLYVVFALVAMTYTS
                     SRKVHMAYGVWTGIALMAMTAMFLTLVGGHRA"
     gene            complement(12882..12971)
                     /locus_tag="PK224_15270"
     tRNA            complement(12882..12971)
                     /locus_tag="PK224_15270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12935..12937),aa:Ser,seq:tga)
     gene            complement(12997..14283)
                     /gene="serS"
                     /locus_tag="PK224_15275"
     CDS             complement(12997..14283)
                     /gene="serS"
                     /locus_tag="PK224_15275"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRB17155.1"
                     /translation="MYDLRVLRDNLDKLRDQLGARGKDVAWEDLRTLTEDRRTRTIQV
                     EELRHQLKKGSDDIARLKREKQPADEAMAAMTALGETIKGHEEQLRVVEEQLAHIALR
                     IPNLPHASVPDGRDEADNIEVRRWGTPPQLSAPAQSHWDLGEALGILDFDRAVRMAKA
                     RFAVLTGSGARLERALINYMLDMHTTQHGYREVLPPLLVNRTAMTGTGQLPKFEDDLF
                     QLQDEELFLIPTAEVPVTNLHREEILAEESLPIRYTAYTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFAKPDQSYDELERLTSHAEAILQGLGLHYRVITLCKGDMGFSAAKTY
                     DIEVWLPSQQTFREISSCSNFEAFQARRANIRCRAKAGKKDNKPEFVHTLNGSGLAVG
                     RTLVAILENYQQPDGTVTVPTVLQPYMGGIQTIERS"
     gene            14405..15421
                     /locus_tag="PK224_15280"
     CDS             14405..15421
                     /locus_tag="PK224_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRB17156.1"
                     /translation="MAVKLVSNPPNRFEPTQREALEPSSRVEIQLFEDDTHQILSRND
                     SPDLPFRWSVNPYRGCFHACAYCYARPSHEYWGFGAGTDFDSKIVLKRRAADLLKQAF
                     EKRSWDGELIVFSGNTDCYQPLESTLGLTRACLEICAAYRNPVGIITKGALPVRDLDL
                     FRQLQEQAWIRVYFSIAFADDETARQVEPQAPTVSKRFETMRILSEAGIPTGISVAPI
                     IPGLNEEAIPELLSRAKSAGATSATCSLLRLPGHVETVFLDRMREAFPDRISKIIHRL
                     QEVRGGQLSEGGFFSRHHGNGTYWRCVEQLFDVGRRRAGFVEEDRPVPRTFRRPTAQQ
                     SLFD"
     gene            15432..15812
                     /locus_tag="PK224_15285"
     CDS             15432..15812
                     /locus_tag="PK224_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRB17157.1"
                     /translation="MSHNPGFLKVVEEARGRVRECTVADVKARLDRAERFHFVDVRED
                     DEYAQDHAAGAIHLGKGVIERDIETVLPNKQEPIVLYCGGGYRSVLAADRIRLMGYTN
                     VISMDGGIKAWRAAGYPIEKGRRP"
     gene            15817..16887
                     /locus_tag="PK224_15290"
     CDS             15817..16887
                     /locus_tag="PK224_15290"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRB17158.1"
                     /translation="MAFSRRELFNNAGFGLLLLPALMRSPRTILGHLLFDPDGPLVPL
                     KPIPENPYRRNGRSIVAIVYGKDTRRMLPRAVDLLGGLDLLGMRGKRVLLKPNVLNDQ
                     PPPSTTNPLVVAAMADMAKANGAAEVVVADGSGIIRLPTSANLTSTGMRAVAEAAGAR
                     VLALEDEPWVKIEVPQAQTLRQFYFSRPVYESDVVINMPVIKTHRFAEYSCSLKNFVG
                     AVHPRYRPSVRFWTGDWHERIAEINLAVHPALTVADGTTTMIEGGPTAGTPSKTDLVI
                     CSGDRVAVDLVAIALLRSFGTWPKLQGKRIGDQRQIKRAAALGLGVGSGQDIELVTEA
                     VDPAPPAFERLVEHIRRELRSV"
     gene            complement(16891..17448)
                     /locus_tag="PK224_15295"
     CDS             complement(16891..17448)
                     /locus_tag="PK224_15295"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRB17159.1"
                     /translation="MGVKWQGAGRRAAVVGVLVAATLGLFSTSPAAEFKMGVIDPQVV
                     LEKSKAGKRALDGLREYVATRQKLLSRDEEELRNTEKQIKEQASKLTDNEKKEKETSF
                     RAKIQDYQKRAQEFNQELQGKQKELVDEYMKKIAVATQAVADKAGYQLVLDKGSEQTV
                     KIVIFNKDTIDLTEQVIKEFDRTNK"
     gene            17650..18054
                     /locus_tag="PK224_15300"
     CDS             17650..18054
                     /locus_tag="PK224_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17160.1"
                     /translation="MRPSEQQQTHRLFVHALQHEAISCEHAGCPGPVETVDQSQTRDR
                     VKTFDLRCERCGWHTSLTGQEQLTPPWDNAALELMADEHLMHQQPVCPFDETPVVFIS
                     MPNPRRKAKYRVACFYCGRQIVMDWPPPESKR"
     gene            complement(18281..19879)
                     /locus_tag="PK224_15305"
     CDS             complement(18281..19879)
                     /locus_tag="PK224_15305"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRB17161.1"
                     /translation="MSETPSPANPVPTQDNAHPILTHERSLANKIAKGSEAGDILDQQ
                     LAMLGIQLVTQLNVLIKTSRIHGRTNAALDKPVDSMLTLVKTLAADHPVVLRLQNDFL
                     FLGDSHLKMSSQQMAVFASIIEALNKWHIGGVSFASTADSKDFREFAYLFVSLDPDKH
                     SLTDLQEAMQAAGIKGIELEDPRSLQLQHLNDAELAGGNTGARTSGSSNGSEGLKEKR
                     KALAKGGYLKASAALGDLVKNTRGGGTVSFKQAKRAIQNIVELMMQDESTLLGLTNLR
                     CHDQYTHNHSVNVSLLAMALGNRAGYPKVELADLGLSALFHDVGKCAISLDVLNKPGE
                     FTQEEWAVMRSHPIEGVFTLVKSRGINNVPARMAAASFEHHMNYDYSGYPKLKVSWTQ
                     TVASRIITIADCYDAMTSSRVYRREPMSPANVLKFMFGKSGQSFDPILLKLFVNCVGI
                     IPIGSLVRLDSGPLAVVIKPAQDKTNAERPLVRVITDESGTSIEDGPELDLTEQDEAG
                     HYTHSILQLVDNAEYHFDTGRYFV"
     gene            complement(19897..21753)
                     /locus_tag="PK224_15310"
     CDS             complement(19897..21753)
                     /locus_tag="PK224_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB17162.1"
                     /translation="MEPTAPLEEGAPLVPFVKAGVDPEVAAIKQLLKLLDKAAKSART
                     YGTKNPVAQRFFQQFYDDLTSHLATHSRLPLLVQRNQLFFKDEVVYQPERDATGDSFA
                     FKMYSDGIRELSFHQDLTQEDLAYFLDALWGTPTDEAGSAEQPADVEEDDDIVTRLWA
                     RNLDTITLVTAEELVRSSGFGPDELELQTQGYMSMSVTSLRELLDRERAAAAPEPAGQ
                     PGTADQPGGGTSGGGTSRNRRFQANVVGYDVSQEEHDALTQEIQRETERDATAYILDI
                     LTAVLASEPSPALLSKLFDVWDSVLDVLIRGGHWTQIETVLTLLQDADAVRPDLSETH
                     KQQLAGLFEGLSRPERLKAIGLHLNHALHAKTEGLLTLLLMMKQDSIPGLCSLLANLD
                     APSHQAVVMEALQTLARDHSDAIVRGLTDKRPTYVRNLLTLISRWNDIRFADCIEKTL
                     RHPESTVRREALRILATLRPSGNGTKLVGLLSDSDETVRLTAMKVLSSGQYSAGFTVW
                     APFVSADDFHDRSPAEKRAVYLALKQTAADEAVPYWQGLLTEWSWTNRKKKGELALLA
                     ADILGKLATPAAVAALEIGQKKGGAAVRQACTSALSVANRQHRQSIPSAANS"
     gene            21905..22576
                     /locus_tag="PK224_15315"
     CDS             21905..22576
                     /locus_tag="PK224_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB17163.1"
                     /translation="MTASTQLPLIVCFGDSLTAGYQTPGPANAHEQETPYGHVLQECL
                     GHRGRVEISGICGEVTGEMVLRFRGTVLDRKPQMVIILGGTNDLGWNADPAEIMRNLV
                     KMYESARAASIVPVPVTVPSIRVDAGADHPDAAAWLAGHIQRRQQLNRLIADYAGRKG
                     LSYFDLFTATADPDSLMLAEPYSNDGLHLTTSGYRLFGRLLYEQLFAPTAAGSLLTPS
                     RPSGP"
     gene            22573..22887
                     /locus_tag="PK224_15320"
     CDS             22573..22887
                     /locus_tag="PK224_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRB17164.1"
                     /translation="MIRDVTDRDFPREVEQASTPVLVEFWQPGCGHCLALLKQLEQLQ
                     EEVAPVVKIVKMNVQENFQIPAELDISSLPALALFERGEFVRFIGGVGRKDEIRKELG
                     LA"
     gene            complement(23061..24605)
                     /gene="zwf"
                     /locus_tag="PK224_15325"
     CDS             complement(23061..24605)
                     /gene="zwf"
                     /locus_tag="PK224_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRB17165.1"
                     /translation="MSSPTTPVDIKPLPEAITPVEACTLVIFGGSGDLARRRLIPAVY
                     NLLLDGLLPDKYAVIGLGRKPMTDAEFRNLVREGVIAHSRQALVEDTWQAFERHLFYI
                     QGENEDAQTYSALRAKAEQIEQTMQLPGNRIFYLSIPPSSFASVCEGLAKSGLATPPA
                     GSAPYSRIIVEKPVGRDLASAQQINEVTGKVFDESQIFRIDHYLGKETVQNLMVVRFA
                     NSIFEPIWNHKYIDHVQITVSEAEGVGTRASYYEEAGALRDMIQNHLLQLLCLVAMEP
                     PYSLDPNVVRNAKMEVLRCLRPIVGKDVEQYTVRAQYAQGTAHGQPVPGYRREKGVSP
                     DSITETYVAVKAFVENWRWSGVPFYLRTGKALPKRASEVAVQFKDIPQILFNANPANP
                     QPANVLTLRIQPDEGLSLRIISRVPGTRAQTHPVEMDFQYSDVFGCPSPEAYERLLLD
                     VMAGDASRFMRRDAVEASWDWVTKILDGWAQQKLRWLPEYSAGTWGPVEGERMIQNDG
                     RAWRIL"
     gene            complement(24633..25526)
                     /gene="gnd"
                     /locus_tag="PK224_15330"
     CDS             complement(24633..25526)
                     /gene="gnd"
                     /locus_tag="PK224_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRB17166.1"
                     /translation="MELGFVGLGKMGMNMVTRLQQGRHRVVAYDRTPEVVKQAEGKGC
                     IGAASLSDLVAKLAAPRAVWIMVPSGAPTEETIQAIAALLQPGDTIIDGGNTRFHDDT
                     RRAVELKQRGLHYVDVGTSGGIWGLTVGYCLMIGGENEPVRRLTPIFQTLAPEQGWAH
                     MGTHGAGHYVKMVHNGIEYSMMQGYAEGFELMAKSDYRLDLGKIADVWMHGSVVRSWL
                     LELAVGALKQDPKLEQLKGYVQDSGEGRWMIQDAIDKDVPVPTLTAALFTRFRSRQEE
                     SFAEKMLAALRNAFGGHSVRR"
     gene            complement(25683..26489)
                     /locus_tag="PK224_15335"
     CDS             complement(25683..26489)
                     /locus_tag="PK224_15335"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRB17167.1"
                     /translation="MEPQLATSSPQSTPALPSSEELQAELLDIPEPPDMPPAVTPPGY
                     EDALAGAVKYVRAKRGGDLVGIILVGSGARRAVTAHSDIDLIALVKGPADGQEMIRVG
                     DRLVDIRYGEHKTVAEDLAYSPRLAPLLRKGRILFDHEETAAQLIAKAAQRFRQGPPP
                     AGIHERIRLKAECLHWLGKVEDLRDKPNTAQYLLQLFFEDCVTAFFRTRGLWFTAPVD
                     TLRFLASRDPALGELASQFLSAGTLHDRLTAGRRFADLLFRDIPLPPRVD"
     gene            complement(26507..26734)
                     /locus_tag="PK224_15340"
     CDS             complement(26507..26734)
                     /locus_tag="PK224_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17168.1"
                     /translation="MPLEVVSFQLISRILDVTDGLGLNREWVEIPLSPESPGQVRTLP
                     NGKLEIIVDADQPFEEWLGTLEQQIRRAQTT"
     gene            complement(26808..27860)
                     /locus_tag="PK224_15345"
     CDS             complement(26808..27860)
                     /locus_tag="PK224_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRB17169.1"
                     /translation="MSNTPQPIIDLRSDTVTKPSAAMRDAMARAEVGDDVYGEDPTIN
                     RLQEAGAAMVGKRAALFVPSGTAGNQLCLRAQTEPGQEVIVEGQSHIVRYELGAAAAL
                     AGVQLHWVAGRRGLMTAEQVEAAIRPKDPYSIQSALICLENTHNAGGGTVYPLATIRA
                     IHEVASTHRLPMHLDGARLFNAVVASGVSAAEYAQHFDTVTFCLSKGLGAPAGSLIAT
                     DDRLLLERIRRFRRMYGGAMRQAGILAAAGLYALEHNIQRLADDHAHARQLAARLQQI
                     PGISIDPETVDTNILFFDVVGAKWSAKEFVAALKPHGLLLNAVGARSCRAVTHLDVPA
                     EAIEQAAEKIARVLDR"
     gene            27965..>28529
                     /locus_tag="PK224_15350"
     CDS             27965..>28529
                     /locus_tag="PK224_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17170.1"
                     /translation="MERLRRSVARFVQAEPQEIWPLAWSFGYFFCLLCGYYILRPVRD
                     EMAIQGGVHNLPWMMTGTFLTLLAVTPLFGWLSARYSRYRLLLAVYLFFIANLVGLYL
                     LMTGQQYIEWVARGFFVWLSVFNLFVVSVFWSFMADLFTPAQGARLFGMIAAGGSTGA
                     LFGPLFTTGLTYLFPVPVLMLVSALFLL"
BASE COUNT         5427 a         7999 c         9039 g         6064 t
ORIGIN      
        1 gccggttgca ggacctgaaa tctctggaag gtgcgctcgt ccggttccag aaagaatccg
       61 cctgcgtata aattattttt gtcgtcctcc acgatggtat gtctgaacgc attgcgaatg
      121 gaccatttgt cgttgaactg atgggtgagg tcatatccga agcgatacga cgtgcggttg
      181 aacgtactga agtcgccgag ggtcacggaa cggttgcgtg gaatgggccc gttgaagttg
      241 gggagaatgg tcccttgagc cggcaggccg taggggtcgt tgctccaccg tctgagataa
      301 tcggcttcga cggtcagcgt ggttcgtgag ctcatcagcc aggtgatgct cggcgcgatg
      361 gccgcgagat cgcgattggc atagtcgata aaactgccgg ctttctgccc ggctacgttc
      421 aatcgataca agacggtctt atcggcatta agcgggccgg tggcatcaag ctctgatcgg
      481 tagaagttga agtttcccaa cgtgacattg gctgaatagc ctgcattcgg caggggtttg
      541 ttcgtcacga tgttgatcac accgccagga tctccctgcc cgtagaggac ggcggccgga
      601 cccttcagga cttcaagccg gcggatattg tacgtgtcgg aggcggtgaa ttgggcgaag
      661 ggatccagca agccgttgcg aaagtaacta tgatccgtcg cgctgaagcc ccgaatgatg
      721 agcgagtcgt agagggaggc cgcggactcc gtggcgttga tgccggacac gttctccaac
      781 gtgttctgga gccggaaggt gcgctgttct tcgatgacct tgcgcgtgat gacctgaatc
      841 gactgcggca cgtctcgaat cggcgtatcg gtcctggtgg ccgtggtgga ttcctcggcg
      901 atataggtct gggcatcgtc gtcgcgctca cggacgtcct tcaccacgat ctccgggacc
      961 ttcaccgact tctgagcggc ggggcgcgac acagcctgag gcgtgggttc tgtcggcgga
     1021 ctttgaaccg tcggagccgg cacgatcggg aggccggcgg cctgctcgag tgtgacgctc
     1081 ccggcgctcg tggtgcgata ggagaggcct gtgtcctgta acagcatgcg aagggcctcc
     1141 tccggggcat agtctcccga gacgccttgc gtctggaggt cgcgggcgat ggtcgcatcg
     1201 aacagcactt cgatctgcgt cgcgtccgaa aactgaagca gggcggaaga gagaggctgc
     1261 ggggcgatcg caaaggatcg cgagaccgtg gaagacggct cttgcgcgtg gacgaaggag
     1321 ccatggtttg caagcatcag gagaaggctg cagagcagta cgtgaatcgt gatgcaggtg
     1381 cctggtcgag gtgaagcgtc ccgaggggtg cacagcgtcg tgatggttcc gaacattgct
     1441 tggtcctttc cgtcgtggtg cgatgggtga tgcggccgtc ttcacggccc attcactacg
     1501 acggacggga gggaggaaag gtctagtgct cgaagaaaat ttcaggattt cttggggagg
     1561 ccccgataca ggaggatgac gcgatcggcg aacgagacga tccggacggg aaggctttct
     1621 tcgatggcgg gcaggaggtg atccaatcgc tcggtgtcga acgagccggt gacgggcagg
     1681 gttttcaggg attgatcccg gaggatgacg agccggcctg aacggtatcg tgccaattcg
     1741 gcgaggactt ccggtaaggg ttggttctcg aagatcaggc gatggtggcg ccaggccagt
     1801 actcggtcca tgtccgccgt ttcgaccgga tcgatccatc catccggccg ataccgaagg
     1861 cgttctcccg cgtgaacctc ggcggacgtc tctttcccca agcggatccg gatgctgtgt
     1921 tcggtgaccg tgacggtggt gcggtcggag cctgttctca cgttgaacgc cgttcccaac
     1981 gcccggattg tgcccctgcc ggcttcgacc tcaaaggggc gagccgcatc cggcgcgacg
     2041 gtgaagaacg cttcgccccg atggagcgtc agccgccgtc cctgttccgt catcgacacg
     2101 gacaatgccg tatccgtatt cagttgcacg gtcgatccat cggccagggt gaccgttcgc
     2161 tcttctccca ccgccgtgcg gtaatcgccg aacggcaacc aggcagtcgt gatccagaga
     2221 ccgatgcctg cgacgagcac gaggcttgcc gccagcgccg cgacacgccg ccaagagcgg
     2281 gatgttcccc gcggatccgt gatcgtgtcc tctgtaaggt accgatcgaa ctcgcggatg
     2341 tcgtcgggat tcaaacggtc cagcccgtgc cagaatcgct cggcctcccg ataggcatct
     2401 cgatgggaag gatcggagga gagccattca gcgaagcgca gccgctcggt ggccgtgatc
     2461 gtgccggact ccatccgtac cagccactcg gatgcggtcg aggcgagcct gtcgcgttct
     2521 ggtgtgtcca ttggggtgat catggtagcg cgtcgcccgt tgccctgtca tcttccggtt
     2581 cgacaggttc ccactaacag tagacgggcg aggcggggag aacatccatt ctggcgggac
     2641 gttcaggatg acaaatcctg gagtcggctt ctgcaaaagt ccaccgcctt gaccatgtgc
     2701 ttgacgacgg tgctttggga aatgcccagc ttgacggcca cctcggcatg gctcaagtgg
     2761 tgaaacttga tgagcacaaa cacctgccgg cacttgggag gcagttcctc gatcgcctgt
     2821 ttcaggcgcg cgacctgctg tttcgaccag acgacggttt caatcgaggg cgcgggatct
     2881 gcctggtcga tcgggcgttc aggatcgtcg agcgtcacgg tctcgcggta ttgtcgccgg
     2941 cgatggtggt cgatgacgag attcgtggcg atgcgaaaga gaaaggcgcg cggattggtg
     3001 aggacctcgc cggacctgtt ctgcagcagg cgaagaaacg tatcctggac caggtccttg
     3061 gcggtttccg tgcagcccaa gcggcgagtc aggaattgct gcaggtcgtg gccgtgttct
     3121 cggatcagcg tcgacatttc caggtgggta cgtgcttcca ttcagtaggg ttccgtcgca
     3181 gaacagggtt gtgcgatcat gattcgcggc ggttggatag tagtggcggg cagacgcaaa
     3241 agggaatgtt cgatttccag gtgcatttgg atggagccga atatcgaggt gtggttcgag
     3301 cccttgcata cgtcttattc agcagggacc ggactcacaa aggaggcaag tgacatttga
     3361 atcgtggaac tggcgcagtc atgaaatcaa tgcgtccgga tcaggtctcc ggaaagcgag
     3421 cttccgcctc cgaacaagcg atcgatgaga ttcgatcgag tctgggctgt tccggtctga
     3481 ggtggagtcc ccagcgggaa cagatcgtga tggccttcct caagcaggat cacatctcca
     3541 tccgcagctt gtatggactg ctcaatagaa acggtcgtcg agcgccgctc aatacgattt
     3601 atcggacgat gcgagtgctg tgtgacaatg gctttgcaca ggcccgacgg ttcggcgagg
     3661 aaacgcagta tgacaacatt tcagcgaaga gggatcatga tcatctgatc tgcacaggct
     3721 gtggctacat tgtcgaattt gaggagccgg ccatcgaagg gttacggcat caggtcgctt
     3781 cggccaatgg gttccatctc accggccgga atctcgaatt gtacggactg tgtgctgtct
     3841 gccgcgtcga cgaacatccg gtcgttcgga cataatcgct atgaggcgga ttcaatcgct
     3901 gcctgctcgc tagggatcgc gccggcgttg gtgcgcaaca cgcttcaacc accacatgcg
     3961 cagggccgtc agataaagta gcagcggggt caaaccggcg aagaagatga tccagcggcc
     4021 ggtcaagccg aacgcctcac cgttgtgcag ggggaagagc catgacaaca gggtttcccc
     4081 gcgggtgaac tgtctccagt cctggacccg ccgtaccgcg ccgctgtatt gatcgatcca
     4141 cacctcggtt tgccctcccg cctgtcggac ctctcccggt tgacggagcc ccaccgaata
     4201 actgtccctg tcatgctgag gcaagccgag ccacatcggt ttcgcgtcag ggaagacggt
     4261 tcgagctatt gcgatggcct gatcgggcgg aagcttgggc aatccggttt gcggttcgga
     4321 ttgaggttgt agttccggcg acgtgtcccg cacgggtgag aaccagcgca cgactgaggt
     4381 gacggcgtcg gggaattcca aatagaaccc ggtcacggcg aggagagtca gcaagagggt
     4441 accggccagg ccggtgagct tgtgtacatc gtaatgtctt ctgatgggac tcccgccgga
     4501 ttgaaacgac aaggcccggc ggagttttcc cagagcgggc caccagagat acagcccggt
     4561 cccgatcgaa acgaggagaa aaacggccat gatcccgaca aagacctcgc ctggcttccc
     4621 gagcaagagc tcctggtgca attcgtaaat gaacgagacg aaaaaactgc cccactgccg
     4681 gtcactcagg gggcgggcgg tcgatgggtc gacggcaacg acgtgccagc gctcttcggg
     4741 aggtgtggca gcaggatcct tgaaccaggc atggaacgtg tcacgctcgt ggagcggaaa
     4801 gatgagggtg tcggggggag accagtccgg atgcacggtc cgggcgcccg ctacgatgtc
     4861 gctaagggat tgatcgctgc cggcagtccg gatcaactgc tccggattca tccattcgtc
     4921 gatcgtcttg tagaacacca acaggctgcc ggtgaggctg gtgagcacga agagcgcgcc
     4981 gcccaacagg ccgacataga ggtgcaggtt tagccacacc tttcgccaaa ccttgcgggc
     5041 aggctggggc aaggccgcga ccgctggggg cgcggaagtc tgcggacggg acgtgtttga
     5101 aggcacgcgc acgggttgga gctggctgac gatcattgtc tgtctccggt ctccctcggc
     5161 ggccggtgca ggagattcgc gcctgtggcg aacaccatga gccccttttt aataagacgc
     5221 gtgaccagcc tgaaaccaca ccttggagcc acaaggaggg caagggcgga tcggtctgga
     5281 gacagggacc cagagggtaa ggttttgtgt cgctcatgcg tcctaataag aagggacagt
     5341 gaccgcctgc gtgggtagaa accaaccatg gtgaataaag tggcagtctc tcagacgggg
     5401 aaagacatac ggtgtctctg tggcagactc acggctcggc tcgagaagcg aggagtggtc
     5461 gtgaagtgcc atcgttgcgg agagctggtg gtctttcccc tttcaaagtt caagaaatac
     5521 ctcacctgtc cgtcccctga ttccgtgaaa taaccagact gatgacgtgc ttcgtcgcgg
     5581 cggctctggg gtgagctgcg gcccgaggat tgcctgtgtc tgtgagccgg cccgtgggat
     5641 catgaaagga ggaggggagt ctcgatgtga gactcccctg ttgcggttgc ggcagtcgga
     5701 agggcgacga ggtcagtgaa actcgaactt gagggatccg atggctgtaa acggcgcgcc
     5761 cgtgtacacg atctcgcgga agttttgcgc accgctgaaa tagcgatggt cgagaacgtt
     5821 ggtgaaattg atggctgcca acaggttggt cttgttgaag acttcctgct tccgatagta
     5881 gagcgcggcg tccatgcgga catacccggc catctcgaat ggtgcttggc aattggcggg
     5941 atcctgacac tggaaaatgc cgtttcgctt tccctgtgcg tacatcccga taccggcgcc
     6001 gaaaccctgc acgacacctt cctggaaaaa ataggtcgtc cacaaacttc cctggtgcaa
     6061 ggcgctgttc ggcgcccgac tcccctcggc gaacaggaga tccttggtca cacgcgcgtc
     6121 gatgtaggca tagtttgcaa tgatgtccca tccgggcagg atctggcccg cgatgtcgaa
     6181 ctccacgcct ttgctgcgtt gttctcccgt cgcaacggag aagaaaaagc cgttgagcgg
     6241 atcggccgtg agcacattct tcttcctggt atcgaagagc gcgaccgtcg aacgtaaccg
     6301 tcctccgaac gcctgatatt tgatgccgcc ctcataggac ttgccgcgtt ccggattgaa
     6361 taggttgccg ttgatgctcc tggaacctgc gctttgcggc gcgaaagact ccgtatagtt
     6421 ggcaaagagg gtaatcggct tccagggttg ataggccaca ccgactgagg gactaaaggc
     6481 cgaatcggtt ttgttgtttt ggctgctggt ggcggtaaaa tcgtcgggat gattcgtgat
     6541 ggtttgatcg aagaggtcga accgtccgcc gaagtgcaca tgcaggttat ccaacaggtc
     6601 cacttggtcg ccgaagtacg cacccaggat gttgttctgc gtgctggcgt cactgaattt
     6661 cgtgagggcc ggattcaaga ataaccggtc gttggtattg aagatgttga tatagctgaa
     6721 cgcgcccggc gtgctcgtgt cgccgccgaa atcccccgag gccgtggccg atgagttttc
     6781 tcgaccgagt tcgattccga tgatcgtttt atgtttgatc gagccggtcg tgaagttgcc
     6841 atggagttca ttctgcaagt agtggctctg gaccgtcgtc ggaatctcat accttgccag
     6901 attcaagatg ccgtcgctct cggctccgac caggaagttg gattcaaggc tggaatagcg
     6961 gctgctggtg acggcggttc tgaacgcggt tcgccaccgg aacatattgc tgatgtcatg
     7021 ccacagagta atggtcgcct tcccatgatg ggtctccagc ttgcgggagg gatcacccaa
     7081 gaagcgcgag accggtatcg gagcgacccc gttgccgatg gcgacgagcc ctcgatcgat
     7141 aggcgcgcgg tcgtacatgt attcggcttc gaaccgcaac gtcgtccgtg aactcatttc
     7201 ccaaccgaag gtcggggcga ggaagatacg ttcggtcttc acgccatctc gataactctc
     7261 cgccgattcg tacatgccgt tgaagcggta ggtgagcgtc ttgctttcgt tcaggggacc
     7321 accgatgtcg atcgtggggc ggtacagccc gtaactgccg acgatcatct ccgccgaata
     7381 gtagggattt ctcaacggag ctttggtgac ctggttgatg actccaccgg gatcagagcg
     7441 gccgtacagg taggacggcg gaccttttac gacttcgacg ctctcgatgt tgatgatatc
     7501 gcgttgcgca cgggcactga aggtactgtc gtctcggaag ccgtttttga agacattcag
     7561 atcggaggca aagcctcgaa tcatgaaggt accgccttgt ccgccttgcg tgctggactg
     7621 cgagacgccg ctgacgttcc ggagggcttc actgaagcga atcacttttt ggtcgtccag
     7681 gacctgtcgg gtcacgacct ccacggagcg tggtgtgtca tggatcggag cgggaatacg
     7741 cgtggccgaa gaggattcgt cgacggcata cccgcgatct tcgacttcct tcacgacgat
     7801 ttccggcact ttcaccggct tgggtaccgc ttgaatctgg gtgttggggt tgggcggcgc
     7861 tggctcttgg ctcagcggag cgagaggcaa gggcgttgcc gccacgggtt ccaacgtcat
     7921 cgttcgggcc tcggtcattc gatagctaag gccggttcca gcgagcatca gcgccaaggc
     7981 ctcctgcggc gtgtgacgac cgctgacgcc gctggtgctc cttccttcag ccaggtcgga
     8041 tggcagcaag agacgatagt tggcttgatc gccgaatgcg gtgatggcgg accccaaggc
     8101 ctgcggtgga atattgaagt ccaccgtgtc tgatgtctca gcagctgctg tggcctgcgc
     8161 aaggagaagg ggactgttgt tcgatgtggt gagacgctcg cttcgcgctt cagccaggtt
     8221 tccggccgta tgcaccagaa cggccgatac ggagagtgtg acgacgactt gccatgagag
     8281 atgcatcatt ccccctctat tagtccggta tgaataagcg cacgtatccg ggcacgggcc
     8341 gagcgacgcg cactcggcgc ttcattaggt aaagacggac cgggccgaaa aacttgtaat
     8401 cgaaatcgtg tgcgttgaaa aaaaacttga aggggatgac ggaggacagc gtctaattgt
     8461 gaagaggatg gatgacgtct caggaaaact gcggtgtctg ttagtgcaag aggacgaggt
     8521 agggcgtcag ggtggtgtcg tgaattcgca acgtctgttc aatcattcgg agcgcggcat
     8581 ccgggtcgcc aatgtcgaac acacctgtca cgttcagtga gcgaagcgcc gggttcagaa
     8641 tcataatgcg cccggctcgg tatcggttga gctcttcgac gactgcggcc agcggctggg
     8701 cttcgaagat cattttccca cgttgccagg cggcgatggc attggagtcg acggcgtggg
     8761 ccctacccag tccggcctcg gcactgtagg agatctgctc gctttgccgg accaccgctg
     8821 cgggaacctg gcggccgtcc gttgccgata cctgaacggc atgctccgtg acggtcacgg
     8881 tgatggcatt ttggtggcgc tgaaccagaa agctcgttcc gagcgcgcgg gtgctcacat
     8941 ctccgctctg taccgtgaac ggtcgtactg tttcgggagt gacggtgaaa ctggcttctc
     9001 cggtgagcag ccggatcgtt cgctgctgcg gagtgaaggc gacggccact gcggtatcgg
     9061 tgttgagatg gatacgactc ccgtcctcaa gcttgacgtg tcgttgttcc cccactgcgg
     9121 tgcggtaatc ggctgacgcg aggtcgagtc gttcttgaac gattccccat gagagccaac
     9181 cggcggcgag taaaacacaa gcggccagtc cccacttgat cgtgcgtcgt gcatgcggga
     9241 gcgtgccgtg cggcatcgat gccgtcaggt cggccgaacc ggtgcaatcg ccattcggca
     9301 gcaagccgat atcccgccat aggatttccg cctctgtgta ggcgagttgg tgggcggaac
     9361 tttgcgtccg ccaggcagtg cacgccaggc gctcttcatc ggaggcgtga ccggaatgga
     9421 tgcggaccaa ccaagcgagg gcttcgtcga tcagtggaga ggaggcagct ggaggtgtcg
     9481 agtcaggcat ccatcatacc attgtgcaca cgactgcccc tgaacgggat actggtcgat
     9541 gtacgctact ctataagacg gatggcgctc ggctttcaac agttaggatg atctggtgga
     9601 ttcctccgga tgcaagcggg cgcggcagtg ccgcaaggct tggacgatat atttcgcgac
     9661 catactctcg gacaccttca gactgtcggc gatttcccgg tgtgttttcc cttccaacct
     9721 gttgagcagc aaggcccgcc ggcatttggg cgacagttcg cgcatggcga cttccaattg
     9781 ggcgacccgc tgcttggcgt ccaaggtatc ctcggccgac ggggccggac tggctgtttc
     9841 ttcagggggc ggttcgatgc tgagcgctcc ccgacgggtg cgaagttttc gcagattgtc
     9901 tactgcgaga ttggccgcga cccgaaaaag gaaggctcgc gggttttgca catcgattga
     9961 aggcctcatg cgcacgaggt gcatgtaggt atcctgagtg caatcggcgg cgagatcacg
    10021 gcagccgagc ttgtgggtga agaacctcaa gagatcgttg tgatgttctt cgaaggcagt
    10081 gagacaggga agtgtatgtt cattctgcat gatggatcct attccaggcg atagccgatg
    10141 gggatgtgga cgacaacatg ggattgaccg agtggatact tcaactggac cggagacgct
    10201 tgttcgagca gagcgactgc gtcgagatcg agaatcgagt ggcccgatgg cgtgacgatc
    10261 gtaatgtcgc tgatgctgcc gtcttcatgg atcacggctt gaagcatgac attgccctgc
    10321 cagcgacggc ttttggcgaa tgttgggtaa cgtttgctct gttcgacctt tgcgaacaac
    10381 atttcggcga gccatccgta atcggcatgg agtatccgtg agacgatggc ctcgcgggcg
    10441 cgaggtgtcg gacggttgac gagccggatc gattcacgaa tggaaggcat ggaggtcgtg
    10501 tgggatactt cggctgcatc gacgctcgga ggcggtattt cggacggctc cgtcgtggtg
    10561 accggaagat ctacacgtgt gacttgaggc gcctcgtgca ctagttcgtc ggatgtcagg
    10621 tccgctgatg gtgtatgagg gccaggcgag acactgtcga cgaccaggga ggggttctcg
    10681 gtggctagtg ggcggacgaa tccatcttgc gcaggtgcga tgattccgga atgagtctcc
    10741 agcgggcggg ccgcttcggt attggcggcg ttcggacggg accggacctg ccgtggagac
    10801 ttgtccctcg gcatgacgga aggggagacg gacgatgcgg aaaagggggt gggtacgtcc
    10861 gctgcgacag gttgcaacgc cggcgcgctc accattgtaa cttcccagcg gaaggggcgc
    10921 ggctccggga gaatggagaa ttcagccgcg aggacaaacg ccgtcccgag actcaggcca
    10981 tgcagggtca gcgaaaccag ccatcctccc attcgcgcca gagactcgcg tttgtcgttc
    11041 atctcaaacc tgcttccggc gtctgttgtt ttcgaaattc gctcggcgaa catccgatgg
    11101 ctcgtttgaa gaagtggctg aacgcgaacg cgtcgctgaa tcccaccgat tccgcaatct
    11161 gggcgatggt caggctctgg tcgctgagga gtttcgttgc tttggcgaga tgcagttgct
    11221 tgaggtattc ggaaaaggag agccccatat acttgcgaaa gatctcggag gtgtatttct
    11281 ccgagtaccc caggagagtg gagagttcct tcaacgtcag gcgatccccg ctgcgcttgg
    11341 acagcagggc cgacaagcgt gccgccgggg taacggaatt gagaggcgtg atctgtggcg
    11401 tcggccagaa aggttgtggt ttggtccgca ggctgtgcaa tggccgttgc cgtgacgctt
    11461 cgattgaaag cggagggctc ccggatgaga cgatgccgat gatggatgta tcgatagttc
    11521 gtgcccggcg gtcttggtgc ataatttccc ccacaaaaaa taaaaagccc gcgatcgtat
    11581 gaccgtgggc tctgggaatt aaattcctcg ggcctctgct gagagtactg gcagagacta
    11641 tctcgttatt gagtacgatt ctcaatatca caatttagaa aaacatgtca agcgagctgt
    11701 ggagcaaagc gtcaggccac cgagtgatcg tttgaaaatt tgtccggaac ggagcggagg
    11761 attaggcgcg atgtccgccg actaatgtca gaaacatggc cgtcatcgcc ataagtgcaa
    11821 tacccgtcca gacaccatag gccatatgga ccttgcggga gctcgtgtat gtcatcgcca
    11881 ccaaggcaaa cacgacatat aagagcgatc cggcggctaa cgcataccag acgagagaaa
    11941 ggtagggcga cacggtgagt ccacttagga ccgttccgat acatgtaggc gcgccaccgg
    12001 tcaagccgag taacaccacc gtgcgcggcg gaatcggaga tggccccgcc gccccgacaa
    12061 tgccgaatcc ttcggttcca ttgtggaggg cgaaaccagc cacgaggagt ccgctcaagg
    12121 cccattctcc gccggcgtaa ctggttccga tcgcgagtcc ttcacccagg ttgtgaagac
    12181 ccatgcccgc cgcgatgaca aacggaagtg tcagtgcctg gtcggcgtgc cgtcctttga
    12241 ggaattgatg tttttcgagg tggacaaggc cgacaaagct gagaaacagg ctgcccagaa
    12301 acagcagcca cgagaaggga tcgcgcactc ccatcaattc caccgcttcg tgcatcaagt
    12361 cgaagaagag ataggtcagt accccggtgg agagcccgat caggccggct tcccacgtcc
    12421 ggggaagggc acgacgaagc accagggccg ccgcgatgcc caggtaaacc ggaacgactc
    12481 ctgcgaggat acccaatccg acgaggtcga gcatggggcg atcctagcca atatctgtcg
    12541 tgcgtggaat gttcagtatc cggaaactgc tcagtgggaa aagataggta tgaaagtgtg
    12601 agaggtttgt tcgagtgagg cgcttgtcat tcggtggatg caaggcgacg cagtaccgat
    12661 gcatggaggt tggccgactc gcagatgcac cggaacgaat tcccgtgact ctcaaagacc
    12721 cgcagcctgc aatttgcggt caggggccct cgtgctatgc gatgaagatc agaggtcact
    12781 aaccgtgcgg aagtggggtc gcttgtcaaa aaatctggcc catgggatcc gtgaccgact
    12841 ttgctcgcaa atcggcttgc gttgcagcca ctattgaaat atggcggagg gggcgagatt
    12901 tgaactcgcg gataggttac cccatctccg gttttcaaga ccggcacgtt cggccactcc
    12961 gtcacccctc cacaccaggc cgtagcacac tcagcgtcag gatcgttcga ttgtctgaat
    13021 tccacccatg tagggctgta gcactgtcgg gacggtcacc gtcccatccg gttgctggta
    13081 attttcaaga atggcgacaa gggttcttcc caccgccaac ccggatccgt tcagtgtatg
    13141 aacgaattca ggcttgttat ctttctttcc ggcctttgcc cggcagcgaa tgtttgcccg
    13201 ccgcgcttgg aacgcttcga aattgctgca ggaggaaatt tcgcgaaacg tctgctgcga
    13261 cggcaaccat acctcaatgt cgtaggtctt cgctgcagaa aaccccatgt cccctttgca
    13321 cagtgtgatc actcgatagt gcaatcccaa cccctgcaga atggcctcgg cgtgcgaagt
    13381 caatctctcc agctcatcat aggattggtc cggttttgcg aacgcgacga gctcgacctt
    13441 gttgaattgg tggagccgga tgaggccgcg cgtgtccttc ccgtaggatc ccgcttctcg
    13501 tcgaaagcaa ggggtatagg cggtataccg gatcggtaac gattcctcgg ccaggatctc
    13561 ttcccggtgc agattggtga ccggcacctc cgcggtcgga atcaggaaaa gttcttcatc
    13621 ttgtagttga aacagatcgt cctcgaactt gggcaattgc ccggtcccgg tcatggccgt
    13681 gcgattgacc agcagcggcg gcagcacttc ccggtagcca tgctgtgtgg tgtgcatatc
    13741 cagcatgtaa ttgatgagcg cccgctcgag tcgggctccc gatccggtca gtacggcaaa
    13801 gcgggctttg gccatgcgca ccgcacggtc gaaatccaga atgccgagcg cttcgccaag
    13861 atcccagtgg gattgggccg gagccgagag ctgcggcggc gttccccatc ggcgcacttc
    13921 aatattgtca gcctcatccc ggccgtccgg gacagaggcg tgagggaggt tcggaatgcg
    13981 cagcgcgata tgggccaact gttcttccac gacacgaagc tgttcctcat ggcctttgat
    14041 cgtctctccc aaggccgtca tcgccgccat ggcctcatcg gccggctgct tctctcgctt
    14101 cagccgggcg atgtcgtccg atcccttttt cagctggtgc cgtagttctt cgacctgtat
    14161 ggtgcgggtt cgacgatctt ccgtgagcgt tctcagatct tcccacgcga catccttccc
    14221 gcgtgccccc aattggtcac ggagcttgtc caggttgtcc cgcaatacac gaaggtcgta
    14281 catagactct cccctctgaa gcggcgagaa atctaacact ggatgtccgc gtagtcaatg
    14341 aaaccggtcg ccaagcgagg ccggggaaag gtaattgaca acgggtgggg agggcggcac
    14401 catagtggcc gtgaagcttg tatccaaccc acccaatcgc tttgaaccca cgcagcgtga
    14461 ggcgcttgaa ccctcgtcgc gtgtggagat tcagcttttt gaagacgaca cccaccagat
    14521 actgagccgg aatgacagcc cggaccttcc gtttcgatgg agcgtgaatc cctatcgtgg
    14581 ctgtttccat gcctgcgcct actgctatgc caggccatcc catgaatact ggggattcgg
    14641 cgcaggcacc gacttcgact ctaagatcgt actgaagcgc cgtgccgcgg atctcctcaa
    14701 gcaggcgttt gagaagcgat cctgggacgg tgagttgatt gtgttttccg gcaacactga
    14761 ttgctatcaa cccctcgagt ccacccttgg cctgaccagg gcttgtcttg aaatctgcgc
    14821 cgcataccgg aatcctgtcg ggatcatcac caagggtgcg cttccggttc gcgatcttga
    14881 tctgttccgg caactgcagg aacaggcttg gatccgtgtg tatttcagca tcgcctttgc
    14941 cgacgacgag acggcccgtc aggtcgaacc gcaggcgccg accgtcagta aacgctttga
    15001 gacgatgcgc attctctcag aggccggtat tcccaccggc atatctgtgg cgccgatcat
    15061 tccaggcttg aatgaagagg ccatccctga gctgttgtcg cgtgccaagt cggcgggagc
    15121 gacctcggcg acctgcagtt tgctgcgttt gccggggcat gtagaaacgg tatttctcga
    15181 tcgcatgcgc gaggcgtttc ccgaccggat ctcgaaaatc atccaccgac ttcaagaggt
    15241 gcgcgggggg cagctgagcg agggcgggtt cttcagccgg catcatggca acgggaccta
    15301 ctggcgttgt gtcgaacagt tgtttgacgt gggccgccgt cgggccggat tcgtcgagga
    15361 ggaccgtccg gttcccagaa ccttccgccg accgacagcg cagcaatcat tgttcgatta
    15421 ggaggagagt cgtgagtcat aacccggggt ttttgaaggt cgtggaggag gcgcgggggc
    15481 gtgtgcgcga atgtaccgtc gccgatgtga aggccaggct ggatcgcgcg gaacggtttc
    15541 attttgtgga cgtccgcgaa gatgatgaat atgcgcagga ccatgcggcg ggggcgattc
    15601 acctcggcaa gggtgtcatc gagcgggaca ttgaaacggt gctgccgaat aagcaggagc
    15661 cgatcgtgtt gtattgcggg ggcgggtacc ggtcggtgct ggctgcggac aggatacgcc
    15721 tcatgggcta caccaatgtc atctcgatgg acgggggaat caaggcctgg cgcgcggccg
    15781 ggtaccccat cgagaagggc cggcgtccgt aatcccatgg cgttttccag acgcgaattg
    15841 ttcaataacg cggggttcgg tctcctgttg ctaccggccc tcatgcgctc ccccaggacc
    15901 attctcggcc atctgctgtt cgatccggac ggccctctcg tccccctcaa accaatcccc
    15961 gaaaatccgt atcggcggaa cggccggtcg atcgtcgcga tcgtgtacgg caaggatacc
    16021 cgtcgtatgc ttccacgcgc cgtcgatctg ctgggaggac ttgatttatt ggggatgcgc
    16081 gggaaacgcg tgctgctgaa gcccaatgtg ttgaacgacc agccgcctcc ttcgaccact
    16141 aatccgctgg tggtcgcagc catggcggac atggccaaag cgaacggagc cgccgaagtg
    16201 gtggtggccg atggatcggg aatcatccgg cttcccacgt ccgccaacct gacgagtacg
    16261 ggcatgcgag ccgtggcgga agcggccggg gcccgcgtcc tggcgctgga agatgaaccc
    16321 tgggtgaaga tcgaggtccc tcaggctcag accctccggc agttctattt ttcccggccg
    16381 gtctacgagt ccgatgtggt gatcaatatg ccggtcatca agacccatcg gtttgcagag
    16441 tattcctgta gcctcaagaa ttttgtcggg gcggttcatc cgcggtatcg cccatctgtg
    16501 aggttctgga ccggtgactg gcacgagcgc attgccgaga tcaatttggc cgtgcacccc
    16561 gcgctgaccg tggccgacgg gacgacgacc atgatcgaag gcggtccgac agccggcacc
    16621 ccgtcgaaaa cggatctggt gatctgcagc ggtgaccggg tcgcggtaga cctggtggcc
    16681 atcgccctgt tgcggtcctt cggtacctgg ccgaagttgc agggcaagcg aatcggggat
    16741 cagcggcaaa tcaagcgcgc ggcggcgttg ggactggggg tcggatcggg gcaggacatt
    16801 gagcttgtta cggaggcggt ggacccggct ccaccagcgt ttgaacggct ggtggagcat
    16861 attcggcggg agctgcgtag cgtgtaagta ttatttgttc gtacggtcga attccttgat
    16921 gacctgctcg gtcaaatcga tggtgtcttt gttgaagatc acgatcttca cggtctgctc
    16981 actgcccttg tccagcacca gctggtaccc agctttatcg gcgacggcct gggtggcgac
    17041 ggcaatcttc ttcatatatt cgtcgacgag ttccttctgc ttgccctgca gttcctgatt
    17101 aaactcctgg gcgcgcttct ggtaatcctg aattttggcg cggaaggagg tttccttctc
    17161 tttcttttcg ttgtcggtca gcttggaggc ctgctccttg atctgctttt cagtattacg
    17221 tagctcttcc tcatcccggg acaggagctt ctggcgtgtc gccacatact cccgcaaccc
    17281 gtccagcgcc cgcttaccgg ctttgctttt ttccagaacc acttgaggat cgatgacccc
    17341 cattttaaat tccgccgccg gactcgtcga gaacaggccg agggtcgctg cgacgagaac
    17401 tccgaccact gccgcgcgcc ggcctgctcc ttgccacttc acacccatct cgggactcct
    17461 ttcgccgaaa aaaatgtctg ctacaaacct cgggagaata gccgcccgtc gtgatcgtgt
    17521 caaggagggc gccgtcgaag ggacaggcga attgacagga gggatgccgg tggctataag
    17581 cctggcaacg cccgccatcg ttccgacatc cgggtaggtg ttcccaccga gcgggtgaga
    17641 aaggatctca tgcgaccttc tgagcaacag cagacgcatc gactcttcgt gcacgcactc
    17701 caacacgaag ccatcagttg tgagcatgcc ggatgtccgg gaccggtcga aacggtcgac
    17761 caatcccaga cgcgcgatcg ggtgaaaacc ttcgacctgc gctgtgagcg gtgcggctgg
    17821 cacacgagtc tcacggggca ggagcaactc acccctccct gggacaacgc cgcccttgag
    17881 ttgatggccg acgaacatct catgcatcag cagccggtct gtccgttcga cgagacgccg
    17941 gttgtgttca tctccatgcc gaatccgcgc cgcaaggcta aatatcgggt ggcctgtttt
    18001 tattgcggca ggcagatcgt catggactgg cctccgcctg agagtaagcg gtaacgattc
    18061 agggggccga cgagggtgaa gaggcggggg aggcgcggac gcgctagccc ggattattca
    18121 tgaatgccgt cgcgaggcgt tcgagacgga agcctgccga ggctgctcgc acgtgagccc
    18181 gacgcaggcg tactcgcagc ccgtcgagga gaacgaacga gaacagcctc gccgggcgag
    18241 agaatcggac accggagcag gtattcttgg atagtccggg ctaaacgaag taccggccgg
    18301 tgtcgaagtg atattcggcg ttatcgacga gctgcaggat actgtgtgtg tagtgtccgg
    18361 cctcatcctg ctcggtcagg tcgagttcag gaccgtcctc gatggatgtg ccgctctcat
    18421 ccgtaatcac tcgaaccaac gggcgttcgg cattggtctt gtcctgcgcc ggcttgatga
    18481 cgacggccag agggccggag tcgagtcgga cgagactgcc gatcgggatg atccccacgc
    18541 agttgacgaa gagcttgagc aggatcggat cgaacgactg gccgctcttt ccgaacataa
    18601 acttcaacac gttcgcgggg gacatgggtt cccgtcggta cacccgggag gatgtcatcg
    18661 cgtcgtaaca atcggcgatc gtgatgatcc ggctggcgac cgtttgagtc caggaaacct
    18721 tgagtttggg gtatcctgag tagtcgtagt tcatatggtg ctcgaacgag gcggccgcca
    18781 tccgcgccgg cacattgttg atgccgcgtg atttcacgag ggtgaacacc ccttcaatcg
    18841 ggtgcgaccg catcacggcc cattcttcct gtgtgaattc tccgggtttg ttcaacacgt
    18901 ccagggagat ggcgcatttc cctacgtcgt ggaacagcgc cgacagcccg aggtcagcca
    18961 gctcgacttt cggataaccg gcccggttgc ccagggccat ggcgagcagt gagacgttga
    19021 cggagtgatt gtgcgtgtat tgatcgtggc agcgcagatt ggtcagtccg aggagggtcg
    19081 attcatcctg catcatcagc tcgacgatgt tctgaatcgc gcgtttggcc tgtttgaagc
    19141 ttacggtccc gccgccgcgc gtatttttga ccaggtcgcc gagcgcagcc gatgctttca
    19201 gatatccgcc tttggccagg gccttccgtt tctctttcaa cccctccgag ccgttcgatg
    19261 agccacttgt acgtgccccg gtattgcctc cagccaactc cgcgtcgttc agatgctgaa
    19321 gttgcagcga gcggggatct tccagttcga tgcccttgat gcccgcggcc tgcatggcct
    19381 cctggagatc ggtaagggaa tgcttgtccg gatccaggct gacgaagaga taagcaaact
    19441 cccgaaaatc ctttgagtcg gccgtcgacg cgaacgacac cccgccgatg tgccacttgt
    19501 tcaaggcttc gataatgctc gcaaacacgg ccatctgttg cgagctcatt ttcaggtgac
    19561 tgtctcccag aaacaaaaaa tcgttttgca gccggaggac gaccggatga tctgccgcga
    19621 gggttttcac cagcgtgagc atggagtcca cgggtttgtc gagcgcggca ttcgttcgtc
    19681 catggatccg cgatgtcttg atgaggacgt tcaactgggt gacgagctga atccccagca
    19741 tggccagttg ctggtcgagg atgtccccgg cttctgagcc tttggcgatc ttattggcca
    19801 gggaccgctc atgagtgaga atcggatggg cgttgtcctg agtcgggacc ggattggcag
    19861 gggaaggcgt ctcgctcaca ggtcgtctcc tcgcaatcag gaattggctg ccgatgggat
    19921 ggactgccgg tgttgtcgat tggcgacgga gagcgcggag gtgcaggctt ggcggacggc
    19981 tgcgccgccc tttttctggc cgatttcgag cgccgcaacg gctgcgggtg tcgccagctt
    20041 gccgagaata tccgccgcca gcaaggccag ttctcctttc ttcttccggt tggtccagga
    20101 ccactccgtc aacagtccct gccaataggg cacggcttca tcggcggcag tctgtttgag
    20161 ggccaggtag accgcccgct tttcggccgg tgatcggtca tgaaagtcat cggcggagac
    20221 gaacggggcc cagacggtga aaccggcact gtactgtccg ctggaaagca ccttcatggc
    20281 cgtgaggcgg acggtttcat cactgtcgct cagcaggccg actaacttgg ttccgttgcc
    20341 ggaggggcgg agcgtggcca ggatccggag cgcctcacgc cgcacggtgg attccgggtg
    20401 ccggagcgtt ttctcgatgc agtcggcaaa gcggatgtcg ttccatctgg agatgagcgt
    20461 cagcagattt ctcacgtagg tcggccgttt gtcggtcaag ccgcgcacga tcgcatcgga
    20521 atggtctcgc gcgagcgtct gcagcgcttc catgacgacg gcttgatgcg aaggcgcatc
    20581 taggttggcg agcaacgagc agagtcccgg gatcgaatcc tgtttcatca tcaggaggag
    20641 ggtcagcagg ccttctgtct tggcgtggag ggcgtgattc aggtgaagtc cgatcgcctt
    20701 cagccgttcg ggtcggctca atccctcgaa caggccggcg agctgttgct tgtgagtctc
    20761 cgacagatcg gggcgcaccg catcggcatc ctgcaacagg gtcaatacgg tttcgatctg
    20821 tgtccagtgt ccgccgcgaa tcagcacatc cagcacgcta tcccagacgt cgaacagttt
    20881 gctgagcaag gcgggggagg gttcggacgc cagcacggct gtcaggatgt cgaggatgta
    20941 agccgtggcg tcacgctcgg tttcacgctg gatttcctgc gtgagcgcgt catgctcttc
    21001 ctgagagacg tcatacccga cgacgttggc ctggaatcgc cgattcctgc ttgtgccgcc
    21061 gccgctggtt cccccgcccg gttgatcggc tgttcccggt tgtcccgctg gctcgggggc
    21121 cgctgccgcg cgctcacggt cgagtagttc gcgcagcgag gtgaccgaca tgctcatata
    21181 gccctgtgtc tgcagttcga gctcgtccgg tccgaaaccg gacgaccgaa cgagttcctc
    21241 tgcggtgacc agggtgatgg tgtccagatt cctggcccac agccgcgtca cgatatcgtc
    21301 gtcttcttcc acgtcggccg gctgctctgc cgatcctgcc tcgtcagtgg gcgtccccca
    21361 gagcgcgtcg aggaaataag ccagatcctc ctgcgtgagg tcttgatgga agctcaattc
    21421 gcggattccg tccgagtaca tcttgaacgc gaagctatcg ccggtcgcat cgcgctccgg
    21481 ttgatagacg acttcgtctt tgaagaacag ttgattgcgt tgtaccagga gagggagtcg
    21541 gctgtgggtt gcgagatggc tcgtcaggtc gtcgtaaaac tgctgaaaga agcgctgtgc
    21601 gacggggttc tttgtgccgt aggtccgtgc cgatttggcc gctttgtcga gcagtttgag
    21661 caactgcttg atcgccgcta cctccggatc gaccccggcc ttcacaaagg ggacgagcgg
    21721 agcgccttct tcgagcggtg ctgtcggttc catatgtagt cagtcgtacc gtgaggtctc
    21781 tttttcgtca agaaaatagc cggtccgcgc aggtgttccg gaaacctgcc gggcgtcgag
    21841 acgcgccggt cgatcgccgg acgcacgcat tgcgtcgcac tgtccgggac agtagaatcg
    21901 cttcgtgact gcatcaaccc aactgccgct cattgtttgt ttcggtgata gcctcacggc
    21961 agggtatcaa acccccggcc cggccaacgc tcatgagcag gagactccct atggtcatgt
    22021 cttgcaggaa tgcctcggcc accgggggcg ggtcgagatc agcgggatct gcggcgaagt
    22081 caccggcgaa atggtgctgc ggtttcgcgg cacggtgctc gaccgcaagc ctcagatggt
    22141 catcattctc ggaggaacga atgatttggg ctggaacgcc gatccggcag aaatcatgcg
    22201 gaatctcgtg aagatgtatg aatcggcgag ggccgcgtcg atcgttccgg ttcctgtgac
    22261 cgtgccgtcg attcgtgtcg atgcgggagc ggaccatccc gatgccgccg cctggctcgc
    22321 cgggcatatt caacgacggc aacagttgaa taggttgatc gcggactatg ccggtcgaaa
    22381 aggcttgtcc tatttcgatc tgttcaccgc gacggccgat ccggactcgc tcatgctagc
    22441 cgaaccctac tcgaacgatg gcctccatct cacgaccagc ggctatcggc tgttcggccg
    22501 gttgctctac gagcagctct ttgctcctac ggcggccggc tctctcctca caccttccag
    22561 gccgtctggc ccatgatccg ggatgtcacg gaccgcgact tccctcgtga agtggagcag
    22621 gcgtcgacgc cggtgctggt ggaattctgg caaccgggct gcggtcattg cctggccctg
    22681 ttgaagcagt tggagcaatt gcaggaagag gtcgcgccgg tggtgaagat cgtgaagatg
    22741 aatgtgcagg agaattttca gattcccgcg gaactggaca tcagctcact gccggcactc
    22801 gcgctgttcg aacgaggcga gttcgtgcgg tttatcggag gagtcggcag gaaagacgag
    22861 atccgcaagg agctcggatt ggcttaaccc agactattca tgaatacctg ctccggcgtc
    22921 cgatcctctc gcccggcggg gctgttcttg ttcggcctcc tcgacggact gcacgtacgc
    22981 ctgcgtcggc cttacgggcg agcagccccg gcgggcgtcg gtctcaaacg cctcgcgacg
    23041 gcattcatga ataatccggg ttaaagaata cgccaggcgc gcccgtcgtt ttgaatcatt
    23101 cgttcgcctt ccacaggccc ccatgttccc gcggaatatt ccggcaacca gcggagtttc
    23161 tgctgagccc aaccatccag gattttcgtc acccagtccc atgaggcttc cacggcatcg
    23221 cggcgcatga accgtgaggc gtcgccggcc atgacatcca gcagcaggcg ctcgtaggcc
    23281 tccggagacg ggcacccgaa gacgtcgctg tactggaagt ccatttccac cggatgcgtc
    23341 tgcgcgcgcg tgccggggac tcgtgagatg atgcgcaggg agagaccttc atccggctgg
    23401 atccgcagcg tcaggacatt cgccggctga ggattcgcag ggttcgcgtt gaagagaatc
    23461 tgcggaatgt ctttgaattg gacggcgact tcgctcgcgc gcttcggcaa ggcttttccg
    23521 gtgcgaaggt aaaacggcac gccggaccag cgccagtttt ccacgaaggc tttcaccgcc
    23581 acatacgttt cggtgatgga gtcggggctc acacccttct cacgacggta tcccggcacg
    23641 ggctggccat gcgcggttcc ctgcgcgtac tgcgcccgga cggtatactg ttcgacatcc
    23701 ttgccgacga tgggccggag gcagcgcagg acttccattt tggcattgcg caccacgttg
    23761 ggatcgagcg aatagggtgg ttccatcgcg acgaggcaga gcagttgcag gaggtgattt
    23821 tgaatcatgt cccgcagggc gccggcctct tcataataac tggccctggt cccgacgcct
    23881 tccgcttcgc tgacggtaat ttggacgtgg tcgatatatt tgtggttcca gatcggctcg
    23941 aagatgctgt tggcgaaccg gacgaccatg aggttctgga cggtctcttt gcccagataa
    24001 tggtcgatgc ggaaaatttg cgactcgtcg aagaccttgc cggtgacttc gttgatctgt
    24061 tgagcggaag cgaggtctcg accgacgggc ttttcaacga tgatccggga gtagggagcc
    24121 gagccggcgg gcggggtcgc caggccagac ttggctaacc cctcacagac ggaagcaaaa
    24181 gagctcggag ggatactcag ataaaaaata cggtttccgg gcaactgcat cgtctgttcg
    24241 atctgctcag ctttggcacg gagcgcgctg taggtctgcg cgtcttcgtt ttccccttgg
    24301 atataaaaca ggtggcgctc gaatgcctgc caggtgtctt ccaccagggc ttgccgtgaa
    24361 tgggcgatga ccccctcccg caccagattg cgaaactccg catcagtcat cggtttgcgg
    24421 cccagcccga tcaccgcata tttgtccggc aacaaaccat cgaggagcag attgtagacg
    24481 gccgggatga gccgtcgccg ggccaggtct ccggaaccgc cgaaaatgac gagcgtgcag
    24541 gcttcaacgg gcgtgatggc ttcggggagt ggcttgatgt ccacgggagt cgttggagaa
    24601 gacatggtgt ggtggtccgt tgggttaagg cctcagcggc ggacgctgtg tccgccgaag
    24661 gcgttgcgca aagccgccag cattttctcc gcgaaggact cttcctgtcg cgagcggaac
    24721 cgggtgaaca gcgccgcggt gagggtcggc accggcacgt ccttatcgat cgcatcctga
    24781 atcatccacc gtccctcgcc ggaatcctgc acgtagcctt tcaattgttc gagcttcgga
    24841 tcctgtttca gggcgccgac ggccagctcc aacagccagg atcgcacgac gctgccgtgc
    24901 atccacacat cggcaatttt ccccaggtcg agccgatagt cgctttttgc catgagttcg
    24961 aagccctcgg cgtagccctg catcatgctg tactcgattc cgttgtgcac cattttcacg
    25021 tagtggcccg cgccatgtgt tcccatatga gcccagccct gttcaggcgc caacgtctgg
    25081 aagatcggtg tcaatcgtcg gaccggctcg ttctcgcctc cgatcatgag gcaatagccg
    25141 accgtcagcc cccagattcc gccgctggtg ccgacatcga catagtggag gcctcgctgt
    25201 ttcagttcga cggcgcgccg ggtatcgtcg tggaaacggg tgttgcctcc gtcgatgatg
    25261 gtatcgcccg gttggagcag ggcggcgatg gcttgaatgg tctcctcggt cggagcgccc
    25321 gacgggacca tgatccagac ggcgcgagga gcggcgagtt ttgccacgag gtcgctcagc
    25381 gatgcagcgc cgatacaacc tttgccctcc gcctgtttca caacctcagg cgtgcgatcg
    25441 taagccacga cgcgatgccg gccctgttgc aggcgcgtga ccatgttcat gcccatcttc
    25501 ccgagtccga caaatccgag ttccataggt catcctctct ggtggttgcc ggtgtgtggt
    25561 atttggggct gcgcgagtct caaacggtca gtcgccgtcc atacactctg gagtaccgat
    25621 ggtagcacgc ccggacgccg atgcaagcgg atggcggcat tcgagcgcct cgacgggccc
    25681 cgtcaatcca cgcgaggcgg aagggggatg tcgcgaaaca ggagatcggc aaagcgtcgg
    25741 ccggccgtca gtcggtcatg tagcgtgcct gcgctgagga actggctggc cagttccccg
    25801 agtgccgggt cgcgggaggc caggaaccgg agcgtatcga ccggtgccgt gaaccagagt
    25861 ccccgggtgc ggaagaaggc cgtcacgcag tcttcaaaga acagttgcag gagatattgc
    25921 gccgtgttgg gtttatcgcg gaggtcctcg actttgccca gccagtgcag acactccgcc
    25981 ttgaggcgga tgcgttcatg aatgccggcc ggaggcggcc cctgccggaa ccgttgcgcg
    26041 gccttggcga tgagttgtgc cgcagtctct tcgtggtcga acaggatccg ccccttgcgc
    26101 aataagggcg cgaggcgggg agaataggcc agatcctccg cgacggtctt gtgctcgcca
    26161 tagcggatgt ccacgagccg gtctcccacg cgaatcattt cctgtccgtc ggccgggcct
    26221 ttgaccaggg cgatcaggtc gatatcgcta tgcgcggtca ccgcccgtcg ggcgcccgat
    26281 ccgacgagaa taatgcccac caggtcgccg ccgcgtttcg cgcggacata tttgacggca
    26341 ccggcgaggg catcctcgta gccgggcggc gtcacggccg ggggcatgtc gggtggttca
    26401 ggaatatcca ataactcagc ctgcagctct tcagaggacg gaagcgccgg ggtggactga
    26461 ggggaggagg tcgcgagttg tggttccatg tctatccttc gacgtgtcag gtggtctgcg
    26521 cgcgtcgtat ctgttgctcg agggtgccga gccattcttc aaacggctgg tcggcatcga
    26581 cgatgatttc aagttttcca ttcgggagcg tgcggacctg gcctggggat tccggcgaaa
    26641 ggggaatttc cacccactcg cgattgagtc ccaagccgtc tgtcacatcg agaatccggc
    26701 tgatcagttg aaacgagacg acttcgagcg gcacgcatcc ccctctggtg tatttacggt
    26761 ggaggcattt tatgaggggg caggcggact gtcaatgtga ggaggggcta gcgatcgagt
    26821 acacgtgcga ttttctcagc ggcctgttcg atcgcttcgg ccggtacgtc gaggtgcgtg
    26881 acggcgcgac agctgcgggc accgacggcg ttgagcaaca ggccgtgcgg tttgagtgcc
    26941 gcaacaaatt ccttcgccga ccacttcgca ccgaccacgt cgaagaacag aatgttggta
    27001 tcgacggttt ccggatcgat cgagatcccg ggaatctgtt gcagccgcgc tgcgagttgt
    27061 cgcgcatggg catgatcgtc cgcgagccgc tgaatattgt gttccaatgc atagagtcct
    27121 gctgccgcca ggatcccggc ctggcgcatg gcccctccat acattctgcg gaaccgacgg
    27181 attcgctcca gtagcaggcg atcgtcggtt gcgatcaatg agccggccgg cgcgccgagt
    27241 cccttcgaga ggcaaaaggt caccgtgtca aagtgctgcg catactcggc tgccgacact
    27301 cccgaggcca cgaccgcatt gaacaaccgg gccccgtcca ggtgcatggg aagcctgtgg
    27361 gtggaagcca cttcatggat ggcccggatc gtcgcgagtg ggtacactgt gccgcctccg
    27421 gcattgtggg tattctcgag acagatgagt gcgctctgaa tactgtatgg atctttgggg
    27481 cgaatagcgg cctcgacctg ttccgccgtc atgagtcctc gcctgccggc gacccaatgg
    27541 agctgcaccc cggccagtgc cgccgccgca cccagttcgt agcggacgat gtgagactgg
    27601 ccttcaacaa tgacttcctg tcccggttcc gtctgggcgc gaaggcagag ttggtttccg
    27661 gcggtgcctg atggaacgaa gagcgcggct cgtttgccga ccatcgcagc tccggcttcc
    27721 tgcagccggt taatcgtcgg gtcttccccg tacacatcgt caccgacttc cgcacgtgcc
    27781 atggcgtcgc gcatggcggc agatggcttc gtgaccgtat cgctccggag atcgatgatg
    27841 ggctgaggtg tgtttgacac ggaggtgctt gctctcgact gatggtggtc gcgcggcatt
    27901 ctagcaaatt ttctgcttgc cggggatggc cggcctgcta cactggggtc ctccccggag
    27961 atgtatggag agactgcgcc gttcagttgc ccggttcgtt caggcggagc cgcaggaaat
    28021 atggccgctg gcctggtcgt tcgggtattt cttctgtctt ctctgcgggt actacattct
    28081 tcggcccgtg cgcgatgaaa tggccattca gggcggtgtg cacaatctgc cctggatgat
    28141 gaccgggacc ttcctgacgc tgttggccgt cacgccgctg ttcggctggt tgtcggcccg
    28201 ctattcccgc tatcgattgc tgctcgccgt ctatctgttc ttcatcgcca atctggtcgg
    28261 cctgtatctg ctgatgaccg gccagcagta catcgagtgg gtggcccgcg ggtttttcgt
    28321 gtggctctcg gtgttcaatc tgtttgtggt gtcggtgttc tggagtttca tggccgacct
    28381 gtttacgccg gcccagggcg cgcgcctctt tggcatgatt gctgcaggag ggagcacggg
    28441 tgccttgttc ggtcccctgt tcacgacggg actgacctat ctcttcccgg ttccggtgtt
    28501 gatgttggtc tcggcgctct ttctcttgg
//
LOCUS       DAORRQ010000054        28446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_105_length_28446_cov_44.900250, whole genome shotgun sequence.
ACCESSION   DAORRQ010000054 DAORRQ010000000
VERSION     DAORRQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28446
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105_length_28446_cov_44.900250"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..862)
                     /locus_tag="PK224_15355"
     CDS             complement(149..862)
                     /locus_tag="PK224_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRB17171.1"
                     /translation="MVKRKPCLIENCDTCGLREKVVLCDISGSDLAEFQKIKRTLEYA
                     PHQTVFYEGHLCLGLYLLCSGKVKLTRSSTRGRRQIVRILGPGELIEKHVFGESPFHE
                     VTCETLEPSQVCVIEKERYLAVIHRNPQLAIKLIQLLSNEVGVNMDHLDQFTFKTARE
                     RLASLLLELGDRFGKKNDDHVRVGLTLKREEVAEMAGITVETAIRLLGVFRDEGVLTI
                     DGRTITLLNPDRLSRIAER"
     gene            complement(958..1191)
                     /locus_tag="PK224_15360"
     CDS             complement(958..1191)
                     /locus_tag="PK224_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17172.1"
                     /translation="MGEGEEGRYSAPQAYGPVRLWLDRGRPAPPSLRLLALMLCRSMA
                     DRSRSEDSPHSLRGQSEEILAHRTGRPEVDGRE"
     gene            complement(1193..2155)
                     /locus_tag="PK224_15365"
     CDS             complement(1193..2155)
                     /locus_tag="PK224_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17173.1"
                     /translation="MKRPRTGITRKRTRLAQALVIGAALFALAPAARALDTQDIVVEW
                     TEEGKKIAQERVAKWKTKEEMVLIPAGEFLMGSDKKTDRLAYRSEIPQRSVYLDAFMI
                     GKYEVTALEYLKFVLATDRLPQLDWRYDGGNFQDTMAHHPIMHVNWYDADAYCKWAGK
                     RLPTEAEWEKAARGVDGRLFPWGSEYAGPTRANFGRTGLSGPVRDRPERLLLYPPIIS
                     VDKYENALSPYGLYQTIGNVSEWVSDWYDQDYYKTAPGRNPKGPETGTQKAFRGGGWM
                     DSTTTMRAAMRNGTDPKTKINWMGFRCAQDAKEDTGAKVSMAKE"
     gene            complement(2188..3120)
                     /locus_tag="PK224_15370"
     CDS             complement(2188..3120)
                     /locus_tag="PK224_15370"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRB17174.1"
                     /translation="MSIWLPGLIPAFVLGIAAGVVFGSVAPSRADHELPKPPPLWSPL
                     DDIERLALSEVPDGMVPVPAGSFLMGSDPKFDRAAGPQELPQHQVYVDAFSIDRYEVS
                     NVNYLRFVLATGAAWPHYWRAQPFPDKMAKHPVIGVSWREADGYCRWRGARLPTEAEW
                     EKAARGEDGRMFPWGNEPAGWIKSNIAHSGSKRGAKYPPLANVDRYDNGVSPYGVHQL
                     AGNVSEWVSDWFDPEYYRRQENRNPQGPASGQDKVFRGGSWNEDPEVARSAGRNAGGL
                     DHWSYLTGFRCATSGESGRQLSSIDPGILRNADH"
     gene            complement(3204..4193)
                     /locus_tag="PK224_15375"
     CDS             complement(3204..4193)
                     /locus_tag="PK224_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRB17175.1"
                     /translation="MTLGTTIQSEQQAMKESSFGWVLALATVALASSLASSPVSAYEE
                     VTVSDGGTLTGVVKLEGTVPKPKGYNLTTLPDPFYCGRISDGEGWRILQPFNVGPGGE
                     FREVVVYLEGIEKGKAFAEKEVPQIEAKDCLFLPFTTVVRDDQSVTVVNMDPVMHDIQ
                     AYETSNLGARVLFNVPLPMNPQHPRNFKDRTEAGMYHKHMAGPPMKELVKLSKNRRIF
                     VMQCGFHAYMESWGVAITNPYFAKTDEQGRFTMTDVPPGTYKLVVWHPYIRTATEQTI
                     TIGPKGTVEATIGVPAPTGRLYANEVLDHAYTRYNVTEEAKKEIDPLLQKQAH"
     gene            complement(4356..9293)
                     /locus_tag="PK224_15380"
     CDS             complement(4356..9293)
                     /locus_tag="PK224_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17176.1"
                     /translation="MRSSVQSKRRRISRKAMLSLQAALLSGSLLAAPWVFAAGPGDQS
                     HEGHATPVAMPGWTQTLKGQTVVENAMEGRAGNAEKMEMQHHRLMEKLEQQAQTDAKA
                     QQTSGAFNEMSMMHQYMGQDGSSFLLASDSSKGEPVMTSGGKCPANAPVKKYDVSMIN
                     IEISLNRWLDFYPGYMYVHTGEIDKVRAEETKNKEAREKDGFDPGAVTTGNQGDAIQP
                     LVLRANQGDCVKMTLRNQMEGEDGSLVIQASSMIVSATGKPATTTNPDTVVSPGKTQE
                     FEWYIHPNMQEGVRQFHSWSHDRELTVLGLFGAFIVEPKGSKYLDPLGTGAAPDTNSG
                     WQVMIDNGSGPDFREFVLFYHEIGDEAFRPLNKKGDFLPQRDPLTDAYRPGGRAINYR
                     SEPFGIDQMHLQHEYFGFEDESLAYSAYTFGDPPTTVARGYLGDPVKWRLVHGGSEVF
                     HSHHPHSGTIRWQRSPRADQEEHWFKGQDGPVKYPVVRTKSDRVDVEVVGPSEALDLE
                     PECGGGGCQHLAGEFLYHCHVAHHYVAGMWGYGRFYNTLQVGNAHTDTMPDMRELPDR
                     LGRMKQGVSSDKLIGTTVDWFGKTFKIVDKSQKTNWKSDPVIVNIKDWVEMFVPAQGQ
                     PGHTNDEKGQIMAYDSTVWDWKWDGNIARGERESTAPNPKYAWAAKWDESTRPAILFD
                     PTTGKMAWPLFKPHFGKRVPFSANHSGAPWLEPIHQDANGERTSEPAMPGEQGRWSLC
                     PDNANRKHYNIHFVRMPITLAKKQGKEPAMVDKDGLIYVLHEEERLTRANDDLKLPAV
                     IRANVYDCVDLLLTSEWDDDDYTNFQSSKINIHPHFFQFDTGNSDGVISGMEYEMSVR
                     PFTMWGKKTKHGLPAPMVAKLVGEVKAGASSVKIQMAPGATAFHVNTELMVGMDCLEK
                     GNDPAASLPRDKSCQEVARVKEIKGDMVTFFKPLKHHHPAGDLVSPEFVRYRWWVDVD
                     MGTVFWHDHAFGATTWPHGGFGVTIVEPFGSTYHDPKNGKLVYSGPIADIHSNEPIGA
                     GVSGSFRELMVSIHDTVPHTVNVIEAGNPPGQPIEVALEAGKTVSFQMPDKILNAPNK
                     YINGGTHTTGSGFNFRAAPFAQRLSNNPDTSKLFSSAIHGDPGTPLLRAYTGDTMVFR
                     LLHQLMNESHVWTIAGHTFLTERYAPEANRKNSIHVGIAERYDLVTKAGGFQGMPGDY
                     IHFNGRTSHFAEGGWGIIRVLDKEVADLKPLPRGTNPLGIPATPNSVCPSDAPVKSFN
                     VVALDRPMKLNPKAPDAIEVDFERKIEMTMPEGKIFALEEEAATVAGNVMPNPLTLRA
                     NLGDCIKVNLKNKMKASRASFFAPGLAFDPKDSQGLNVGNNAGDQTVAPGESRTYTYY
                     AHPANKETTSLVWDGGNIVVNPRNGLYGAIVIGPKGSQYRDPVTGADVSQKNTWRADV
                     IVDTTLSENAGKRNYRDVALFFQDEDNIIGTAFMPYVQNVAGLTSVNYRAEPYKFREE
                     QGCSLGKIFQPCAVDKPEDPVTPLIEAHAGDALRIHVIGANSEQNGMFSVEGHEWPIE
                     PYMAGADMISVVEYAGSEILDVFIRGGAGGPYRQVGDFVWSNARLPYTQSGQWGYLRV
                     LPTGDARIQPLSASGAGARQAQVLPEPQAIPTAMK"
     gene            complement(9868..11271)
                     /locus_tag="PK224_15385"
     CDS             complement(9868..11271)
                     /locus_tag="PK224_15385"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB17177.1"
                     /translation="MAGSHSQGTPFSPFTDPILAARLEALKLLADGLTDRVAVMDRDL
                     NVVYANDAAWAKSGNPSAVAKPAKCYQAFVHMTDPCGTCPAESVFKTGEVHTVSCTTS
                     GDGTACGMRQAFPLVSSAGEVASILVLFHKRPEPLSIPASLPQQRLGRSRPERTESRL
                     GDLVGASPAMNQLFEMIRLVADSSATVLIQGESGTGKELVARTIHQTSYRRDKPFVVV
                     DCGALPETLLESELFGHVKGAFTGAAGAKSGLFEEADGGTIFLDEIADTSPTFQAKLL
                     RVLQEGEIKRVGGTQPVKINVRVISATNKDLTDLVKAKAFRQDLYYRLAVLPVHLPPL
                     RDRRVDIPLLVVKFIADSCQRHRQDLRQVNEEAMRALTAAPWPGNVRELQHYIERAVV
                     TTTHSELTCADLVELGSHPQDTDLRTVGRGAARQAERVRILQALQQTSGNRVQAARVL
                     KISRASLYNKLHELGIQ"
     gene            complement(11415..11846)
                     /locus_tag="PK224_15390"
     CDS             complement(11415..11846)
                     /locus_tag="PK224_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17178.1"
                     /translation="MVTYSEGSNVESRTPAVLLVVEDDKDMRSLLCDELWGEGYQLRE
                     ASNGEEGLAAVMRAAPDLIVTDLKMPAGGFDYVHRLRSCVPGCPIIVMTAFGDAQTKD
                     EAMKNGATAYFDKPVRLSELKATVKQLLQHPGSRSEDGLLH"
     gene            complement(12137..14827)
                     /locus_tag="PK224_15395"
     CDS             complement(12137..14827)
                     /locus_tag="PK224_15395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB17179.1"
                     /translation="MQPLHQKSPLFEYGVSLLLTVGIFVLDLSAPAGIDLWLLYAIPF
                     TLIAVSSLGQVPRYLMGLVALLALIGPLASSAGSLPLSSWLNRLLGIGIMGAVAALVA
                     GRRSSPASTNASPGLSPQAFTTARAERHQPEAGEVSDAEARARAEAAVVGAVAGQRRA
                     EAELHQDKLRFEGIVHSAMDAIITVDEAHKIVLFNQAAEKMFQWGAEEVVGRPLDRLL
                     PERFRSTHDEHIREFGRSGITTRQMGALGMVMGVRANGEEFPIEAAISQIGVEGTRYY
                     TVILRDITERKRLEQELAEREALLRAIIETEPECVKVLDLDGSVRTINAAGLAMIEAT
                     SSADIVGRDVCHLATAEFRSVYRDLIGKAGRGEAGRLEFQMVGLRGTPRWLDTHMVPL
                     RSADGIVTAVLGVTRDITEWKKSETLLRQSEERYRRLLAVLPDAILVNRENRIFFANE
                     QGVRLFGARSAEEILGKSLYDLAHPDYHEMIDARIRHLLGPGNTVPEVEEKIVRLDGM
                     SVDVAVRAARFQDEEGFGILVVLRDISLRKAAEQKLRESEERLQSLLGAMEDVIWSSS
                     LDLSTMFYVSPSVMEIYGRPPDAFLARPSLWLEAVHPDDCVIAEQARQALSTMGEFDV
                     EYRIVRLDGDLRWLHDRGRVIKDADGRPLRIDGIASDITERKRLQAQLRRTERVAELG
                     TVASGMAHEIGTPMNVILGRAEYLMERTKEEPVRKGLQTIVSQVERITRVMNQLLAFA
                     RRRPVEHRALDLRQTIEDNLEIFQERLSQSNISVETSFADACPLVHADSDQMSQVLIN
                     LVMNAIHAMPGGGVMKLSLAPDRGMVRLTVADTGHGMPRDVIAKVFDPFFTTKEFGKG
                     TGLGLTVVKGIIEEHSGTIEVESEPERGTTFTICLPIDKS"
     gene            complement(14864..16243)
                     /locus_tag="PK224_15400"
     CDS             complement(14864..16243)
                     /locus_tag="PK224_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB17180.1"
                     /translation="MTEEWGAVLVVDDDADMRSLLCDVLQGRGHQCTGVGSGDDALQR
                     LSEEDYAVVLTDLRMKGMLGTELLAEVKHRHPDIGVILMTAFGSVETAVEAIRHGASD
                     YLTKPVKTEELVRVVERAIREAALRREVSRLRKEVHKEYSFHQILGKSKPMQAVFDLI
                     RRVADSPTNVLITGESGTGKELVAKAIHYNSDRRDAPFVPVNCAAIPEQLLESELFGH
                     MRGSFTDAKMDKRGLFEEAQKGTLFLDEISELPLMLQAKLLRAIQEREIRRVGATKSI
                     PVDVRIIAATNLNLSEEVKNKRFREDFYYRLNVIELRLPPLRERREDIPILVDAFLKK
                     CADSRGKQVKGLSESALAMLADYAWPGNVRELENVIERAVTLSHGDMIGADDLPVQVQ
                     GSRGDRRILDEAAERTLPLHEVEKEYIVKILEKTGGNKYQAAHVLGIDRKTLYRKLAE
                     IEGKPHSDA"
     gene            16505..17824
                     /locus_tag="PK224_15405"
     CDS             16505..17824
                     /locus_tag="PK224_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17181.1"
                     /translation="MESDHSKQDPRPTTRPAPVVLAPVPVVQGQGHAQVPVRVGACPL
                     PGRKRRTPLLLAAVGLLAILALGLWHWWSNDPVAGHYKTLPVDRGPITSLVTATGAVN
                     PVISVQVGSQVSGKIAKIYADFNSIVREGQVLASIDQKPYQAKVSQAKAALKSAKATL
                     AKARNMLAQRTLELNRMAALREQQFVAQSDLDLSRTNYRDAEAQVDLSQAQVDQAKAT
                     LSSAELDLGYTTIISPVNGTVVSRNVEEGQTVVASFQTPTLFVIAQDLTRMQVIANVS
                     ESDIGGVSEGTSADFRVDAYPREFFHGVVTQVRNAPVSIQNVVTYDVIISVENPELKL
                     KPGMTANITIVTARNEDALRAPNAALRFRMPGVPVDRKTAQLWVLEPTGRVRAAPIST
                     GIADSLFTEITTGEAHEGDAAIVGITSAEDSAQEKLPPGFEFGPKMR"
     gene            17835..19067
                     /locus_tag="PK224_15410"
     CDS             17835..19067
                     /locus_tag="PK224_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB17182.1"
                     /translation="MSFLWLTIQSALRVLRRNPLRAGLTMLGIVIGVGAVVGMVSLGQ
                     GATASVQREIASLGTNVLIIIPGATTTGGVRGGLGSVSTLTVDDARDIEKRIGDISLV
                     TYASRSVLQVIHEHKNWNTIALGTTTAFPDLRNWPVADGNFFTQADEDAAAKVVVLGK
                     TVADNLFERGEEVIGAQIRVKNVPLRVIGILSPKGQSLSGQDQDDIVVLPFTTAERKV
                     LGTKFLGTVGIIMVATQHRYSIPAAVEEIKELLRARHRIHPAEENDFTIRTMEDVAKT
                     VAGASRTMMVMLLSIASISLIVGGIGIMNILLVSVTERTREIGIRMAVGAKRAHILLQ
                     FLVEAIILTAIGGVAGVIFGIAGARLLTRLIGWPTIISSQAVAVAFLFSLAVGIFFGL
                     YPANKASRMNPIDALHWE"
     gene            19219..20322
                     /locus_tag="PK224_15415"
     CDS             19219..20322
                     /locus_tag="PK224_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17183.1"
                     /translation="MKKTKEYPHTRFPPEVLRKAREKIESWTEEQSGNSSALQASIQI
                     DEQEKWQFDNEDDFFPHYRKEHSYSYYDVTVGNMHLTIEVYRHTTRIAVQAGARSKIE
                     SVFSIFEDASSTSRCLDEKQPQSIFSRQPGFSTDRKLSSLNVDKNLLLRLEDYFLEEI
                     PRIAKLKPEEARRNYKITSADHAGVQQFRSIREYSGDVFPDTISRVVLNSGFIASDDA
                     SVNVKLSLDTEPHWSSVQVDVTSANNPRELCHAVLQGLLDRLEPNKNLHRLLHPSFPL
                     TGILYGLSAPSGMAALTLAGKGLYLPACLAAVPPTILFLLWIGGKANPYTAFKTQAND
                     RWSDLWKWFCLGLLGFLLFGTGAFYLRKALLGY"
     gene            complement(20553..21461)
                     /gene="lipA"
                     /locus_tag="PK224_15420"
     CDS             complement(20553..21461)
                     /gene="lipA"
                     /locus_tag="PK224_15420"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRB17184.1"
                     /translation="MSFIHIDPRPTVDGNQPSAPQSRRLPSWFKVKLQTGPDYHDIRK
                     TMDRLNLHTICEEARCPNMWECWNARTATFLILGDICTRRCHYCSVATGRPHEVDREE
                     PLRVAEAVQALNLRHAVITSVNRDELDDGGASVFAETIRHIRRLIPSCTIEVLIPDFE
                     GNEGALALVAAEKPDILNHNIETVRRLFPSIRPQGKYQRSIELLGRAKQMGMTTKSGL
                     IVGMGETTDEAREVMRDLRSVECDIMTIGQYLQPTKEHLSVARFYHPDEFAGLKEEGL
                     AIGFRHVESGPLVRSSYHAEQQVSGR"
     gene            complement(21458..22660)
                     /locus_tag="PK224_15425"
     CDS             complement(21458..22660)
                     /locus_tag="PK224_15425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRB17185.1"
                     /translation="MASRVVMPKLTDTMEEGVLLAWKKREGDQVHAGEVIAEIETDKA
                     VMDLEAFAPGILRKILVRDGETVQSGTLIAVIAEADEDIASALSDGVTAAPSIGNGAK
                     TGAAPGVAPAPTAAARPEGARPFASPRAKALAAERGIDLSALTGSGPGGRIVEDDVMQ
                     ATAQPAQALLAGTDQPLSQMRKAIARATVQSKAPVPHFYLTVEIDMEQAERVRDQFKQ
                     SRQTHPSITDLLIKASALALRRHPEINISFAGDAIRRFEQIDIGVAVGMEDGLITPVV
                     RDCGAKTLAEISVEAKALIERARQKRLQPNEYTGATFAISNLGMFDVDNFIALLMPPQ
                     AASIAVGAIRDVPIVTKGTVTAGRRMKVTLSCDHRALDGLMGAQFLKEFKRVLEHPQE
                     LVAPVVKP"
     gene            complement(22724..23701)
                     /locus_tag="PK224_15430"
     CDS             complement(22724..23701)
                     /locus_tag="PK224_15430"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HRB17186.1"
                     /translation="MLLSYREALNQAMREEMRRDPRIFLIGEEVGYYQGAFKVTKGFV
                     EEFGPQRVVDTPITEAGFTGLAIGAAMAGLQPIVELMTMNFGIVALDQIVNNAAKIRY
                     MSGGQLSVPIVIRGPGSAAHQLGAQHSQSLEAWFCHVPGLKVVAPSTPRDAKGLLKSA
                     IRDQNPVIFIEAQLLYGTKGEVTEGEYTIPLGQAEVKREGADVTVVAYSKMVLVAMEA
                     ADQLSRDGLDVEVIDPRTLKPLDLDTIVTSVKKTGRLVIVEEGWRFCGLGAQIADSIY
                     TAAFDYLDGPIVRVTGEEVPMPYSRPLEDAAIPDVPRVVAAVKSVCGAA"
     gene            complement(23709..24677)
                     /gene="pdhA"
                     /locus_tag="PK224_15435"
     CDS             complement(23709..24677)
                     /gene="pdhA"
                     /locus_tag="PK224_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRB17187.1"
                     /translation="MDRDDLLSLYRQMLLIRRFEEKSAEMYALAKIAGFLHLYIGEEA
                     IAVGAIAALRPDDYAISAYRDHGHCLARGSDPGKVMAELFGKATGLCQGKGGSMHLVD
                     LAHRFMGGYAIVGGHIPLATGLAFATKYQKQDLVTVCFFGEGAVPSGQAHEAFNLAAL
                     WKLPVIFICENNRYGMGTPVHRAVALYENVAEAARSYGIMAERVDGMDVLAVRALMRT
                     VVDQVRAGHGPFFIEAMTYRFMGHSMADPSHGHYRSKDEVEEHRKRDPLVLLKQQILD
                     EALCVETDFKPLEQEVGDIVAAAVKFADESPFPEPSALHRDVMVED"
     gene            complement(24833..25507)
                     /locus_tag="PK224_15440"
     CDS             complement(24833..25507)
                     /locus_tag="PK224_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17188.1"
                     /translation="MRANYCSEGRRRRSHGVIGLVVGAMVWAVGGPAWAIDIKLSPEE
                     AKRALEAGRAPMEKANSPEDVKKVLQQASMVTRVGADPEKDQCGASAILRTKRYRLEA
                     FGRQEAAESKKQKKDVRMPEEFIQKVVDMPNMEVEVQLCGDDEYFAEKADIVFQQKGK
                     NIRPVDISPADRGRKNEGAGPAYRSRFTARFGYESFDPNAKTTVIVTLQDGQTMQFDA
                     DFSKVK"
     gene            complement(25778..26410)
                     /locus_tag="PK224_15445"
     CDS             complement(25778..26410)
                     /locus_tag="PK224_15445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRB17189.1"
                     /translation="MRLILASTSPRRHELLTLLGLRFDVVAPPFVEQVMPNISAEQQA
                     VEFATGKVRSCIELCPDALILGSDTLISLGSEVLGKPADLADAESMLRRTAGKTHKIF
                     TAVALMDVEPESCQVQTATVLVTMKPLNEEALAAYLRTGDSLGKAGAYSIQGGGAALI
                     ERIDGDYTAAVGLPLRMVAEMLRRCGIACPVDVDQLYARKPYPNWARFNP"
     gene            complement(26414..27598)
                     /locus_tag="PK224_15450"
     CDS             complement(26414..27598)
                     /locus_tag="PK224_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRB17190.1"
                     /translation="MSFWATLPQPILGLAPMDGVTDAAFRRVVASQGRPDITFTEFTN
                     VNEICRGPDHLLDSLIYSEAERPIVAQLYGKDPDQFYRAAQVVCELGFDGLDINMGCP
                     SKSVAASGSGAALIKTPDLAHAIVRAARQGLEDWAGGQSIHSQGFKPSRIEFIHTLNH
                     SRSGVPVVPRRLLPLSIKTRLGFDCVVVERWVEHLLAARPAAITLHGRTLQQMYRGAA
                     DWSAIAEAAKLAHGTGTLMLGNGDLNTLVDAIRRAAESKVQGVLVGRGTLGSPWFFRE
                     KEQARRAFSERDDLSTLPATAWEPPVSVSHRMQVMLDHARQYEAIAGLERFRSVRKHL
                     GWYCKGFPHAAAMRGKMFGASNVQDVERIVAEFCQELMQAERDASAPTPLAAQTSSVS
                     VA"
     gene            complement(27595..>28446)
                     /locus_tag="PK224_15455"
     CDS             complement(27595..>28446)
                     /locus_tag="PK224_15455"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HRB17191.1"
                     /translation="AWLRQTLEAFAAEFLDAFPMERVIEIHVAGLAEFTAQGGAQYMV
                     DAEALPYWIDAHGAPIPEVLFDLLAQVLAHPRLTCLKGVALEVDTKPVATIVTEFRRF
                     CDRFESMVTSVVQAGAAQSLTRPAVEQRTGTRQPLTMDEQASLRNQYQGYVRFVTTGQ
                     ALPPSEHPSLLGGSLDNLDRYRKTYLPHELLHWGGELKDMFPRTCSLLIAAHLPLERF
                     VSFWFSRPRTDEQDYDFFLLKIDRFVEFVAHDCPSAAVTVMEEAEEMRMAYRTANEPT
                     ESAEVRA"
BASE COUNT         5772 a         9085 c         7767 g         5822 t
ORIGIN      
        1 gcaaggggcg agatgagcga gtgctaccgg gagagcggga tggtggagag cccgaggcgc
       61 ggaatgtcga acctggaacc gtgaacatcg catcgcgcct gtcgcgcttc acgaacgaca
      121 tttcacgaat accgtgtaag gaatccggtc agcgctcggc aattctggag aggcggtctg
      181 gattcagcaa ggtgatggtt cttccatcga tggtgagtac gccctcgtcg cgaaagacgc
      241 ccagcaggcg gatggcggtt tcaacggtaa taccggccat ctcggccact tcttcccgtt
      301 tgagcgtgag tcccacccgg acatgatcgt catttttttt gccgaatcgg tcgccgagtt
      361 ccagcagcag gctagctaat cgctcgcggg cagttttgaa cgtgaattga tcaaggtgat
      421 ccatattgac cccgacttcg ttactgagga gctggatcag cttgatcgcc agttgaggat
      481 ttctgtggat gacagcgagg tagcgttcct tctcgatgac gcagacttga gagggctcca
      541 atgtctcgca cgtcacttca tgaaaggggc tctcaccgaa gacgtgtttc tcgatcaact
      601 caccagggcc caagatgcgg acgatctgcc gccggcctcg cgtcgaagag cgagtgagct
      661 tgaccttacc cgaacataac aggtagagac ccaagcacag atgaccttca tagaagacgg
      721 tctggtgagg cgcgtactcg agcgtgcgtt taattttttg aaattcggcg agatcgcttc
      781 ctgaaatatc gcacaggacg accttctcgc gaagcccgca ggtgtcgcaa ttttcgataa
      841 ggcagggctt ccgtttcacc acttgtcgat tcccggcgct agtccaacac cgtgccttcg
      901 aagactgctc gaaacacggt caaaccggac ctttcactcc gaattcagat gaacccttca
      961 ttcccggcca tccacctccg gtcggcctgt cctgtgtgcc agaatttctt cggactgccc
     1021 gcgcaaggag tgggggctgt cttcgcttct tgaacgatcc gccatcgacc gacacaacat
     1081 cagggcgagc aagcggagcg acgggggagc cggtcgtccc cgatccagcc agagacgaac
     1141 cggcccatag gcttgggggg cggaatacct tccttcctcc ccctctccca tactactcct
     1201 tcgccattga gactttcgct cccgtgtcct ccttggcgtc ctgcgcgcaa cggaatccca
     1261 tccaattgat tttggtcttg ggatcggtgc cgttccgcat tgccgcccgc atcgtcgtgg
     1321 tgctatccat ccacccaccg ccgcggaacg ctttctgcgt gccggtttca ggccccttcg
     1381 ggttccggcc cggcgcggtc ttgtaatagt cctggtcgta ccaatcggag acccactcgg
     1441 acacgttccc gatggtttga tacagaccgt aggggctcaa agcattctcg tacttatcga
     1501 cggaaatgat cggcggatag agcagcaagc gctcaggacg atcccgcacg ggcccggaca
     1561 accccgttct accgaaattg gccctggtcg gaccggcata ttcgctgccc catgggaaca
     1621 gtctgccatc cactccacgc gccgcctttt cccactcagc ttcggtcggc agccgcttcc
     1681 ccgcccattt gcaataggca tcggcgtcgt accagttcac atgcatgatc ggatgatgcg
     1741 ccatcgtgtc ctggaagttt cctccatcgt accgccaatc cagctgcggc agacgatccg
     1801 tcgccagaac aaatttgagg tactcgagcg ccgtgacttc gtacttcccg atcatgaacg
     1861 catccagata caccgaccgc tgcggaattt cactccggta ggccagtcga tccgttttct
     1921 tatcgctgcc catgaggaat tcgccggccg ggatgaggac catctcttcc ttggtcttcc
     1981 acttcgcgac ccgctcctgc gcgatcttct tcccttcctc ggtccactca acgacaatat
     2041 cctgtgtatc caacgctcgt gcggcaggag ccagcgcaaa cagcgccgct ccgatcacga
     2101 gcgcctgtgc caacctggtt cgtttccttg tgatgcccgt tcgtggtctt ttcatgtttg
     2161 gcctcctcgt tgtcctgccg tcaaaactca gtgatcggca ttccgcagaa tgcccggatc
     2221 gatggacgaa agctgacgac cactctcgcc cgatgttgcg caacgaaacc ccgtcaaata
     2281 actccagtga tccagccctc cggcattacg cccggcggaa cgagccacct cgggatcttc
     2341 attccatgag cctccccgaa acactttgtc ctgacccgac gcaggcccct ggggattgcg
     2401 gttctcctgg cgccggtaat attccggatc gaaccagtct gagacccact cgctcacatt
     2461 gccggccaat tgatgcacgc cgtaagggct cacgccgttg tcatagcgat cgacattcgc
     2521 cagcggcgga tacttggccc cgcgcttgga acccgaatgg gcgatgttgc tcttgatcca
     2581 gccggccggc tcattgcccc agggaaacat ccgtccgtct tcaccgcgcg ccgccttttc
     2641 ccattcggct tcggtcggca aacgtgcccc gcgccaccgg caatagccat cggcttcccg
     2701 ccaggacacg ccgatcaccg gatgtttcgc catcttgtcc ggaaacggct gggctcgcca
     2761 gtagtggggc caggccgcgc cggtcgccag cacgaagcgc agatagttca cattgctgac
     2821 ctcgtaccgg tcgatactga acgcgtcgac atagacctgg tgctgaggca gttcctgcgg
     2881 tccggcggct cgatcaaact tcgggtcgct tcccatcagg aacgaaccgg ccggcaccgg
     2941 caccatcccg tccggcacct cactcaacgc cagccgctct atatcgtcca ggggggacca
     3001 gagcggcgga ggcttcggca attcatggtc agcgcggctc ggcgcaacgg agccgaatac
     3061 caccccggcg gcgatgccca gcacaaaagc cggaattaaa ccaggtaacc agatgctcat
     3121 tacgcgatca ctccggactc actgatcgga tggatcagtg agtccactga tccacgaaca
     3181 cttcggcact cgcgcgcccc cacttagtgt gcttgctttt gcagcagggg atcaatttcc
     3241 ttcttcgctt cctcggtcac gttgtacctg gtataggcat ggtccaacac ctcattggca
     3301 tagagccgtc cggtcggcgc cgggactcca atcgtggcct caacggtgcc cttcggaccg
     3361 atggtgatgg tttgttccgt cgccgtcctg atatagggat gccagacgac caacttatag
     3421 gtgcccggag gcacatcggt catggtgaac cggccctgtt cgtccgtctt ggcaaagtag
     3481 ggattcgtga tggccacccc ccaactctcc atgtaggcat ggaaaccgca ttgcatgaca
     3541 aagatccgac gatttttgct gagtttcacc aactctttca tcggcggacc ggccatgtgt
     3601 ttgtggtaca tgccggcctc ggtgcgatcc ttgaaattgc gtggatgctg cggattcatc
     3661 gggagcggca cgttgaacag gacacgcgcg cctaaattcg acgtttcgta ggcctgaatg
     3721 tcgtgcatca ccgggtccat gttgacaacg gtgaccgact gatcgtcccg cacgacggtg
     3781 gtaaacggca ggaagaggca atccttcgct tctatttgcg gcacctcttt ttcagcgaac
     3841 gcttttcctt tctcgatccc ctccagatac acgacgactt ctcgaaactc gcctcccgga
     3901 ccgacgttga acggttgcag aatgcgccag ccttctccgt cggaaatgcg cccgcaatag
     3961 aacgggtccg ggagcgtggt gaggttgtac cccttcggct tcggaaccgt cccctccagc
     4021 ttcacaaccc cggtcaatgt cccgccatcc gatacggtga cttcttcata ggctgataca
     4081 gggctgctcg cgagggacga tgcgagggcc accgtagcca atgccaagac ccacccgaaa
     4141 gaactctcct tcatcgcctg ctgctcactc tgaattgtgg tacccagtgt cacgtccggc
     4201 ctcctctgtg attggttcat gatgcgatcg acagatatta tcgatcatac agtgaaccca
     4261 tcacctaatt cctctctcga gactcaaaac acagatgggg gagctgcgtg atacaggcag
     4321 ctcccccatc cggtgttacg ccatgactga cccgcttact tcatggcagt cggaatcgcc
     4381 tgcggctcgg gcagcacttg agcttgacgt gcacccgctc cacttgcgct gagaggctga
     4441 atccgcgcat cgccggtcgg caacacgcgc agatagcccc actgtccgga ctgcgtgtag
     4501 ggcagccgtg cattcgacca cacgaagtca cccacctgac gatacggtcc gcccgcaccg
     4561 ccacggatga agacatcgag gatctcagat ccggcatact ccaccacgct gatcatgtcg
     4621 gcacccgcca tgtacggctc gatcggccac tcgtggccct cgacgctgaa catgccgttc
     4681 tgctcgctgt tcgctccgat cacatggata cggagcgcat cacccgcgtg cgcctcgatc
     4741 aaaggcgtca cgggatcttc gggcttatcg accgcgcaag gctggaagat tttgcccaag
     4801 gagcaccctt gttcttcacg gaacttgtac ggttcagcgc ggtagttcac cgacgtcaaa
     4861 ccggccacgt tctgcacata gggcatgaag gcggttccga tgatgttgtc ctcgtcttgg
     4921 aagaagaggg ccacgtcacg atagttccgc tttccggcat tttcagacaa ggtggtgtcg
     4981 acgatgacgt cggcccgcca tgtgttcttc tgtgacacat cggcaccggt gactggatca
     5041 cgatactgcg atcccttcgg cccgatgacg atcgcgccgt acaatccgtt gcgcggattg
     5101 acgacgatgt taccaccgtc ccacaccaac gacgtcgttt ccttgtttgc aggatgcgcg
     5161 tagtaggtat aggtgcggct ttcgcccgga gccacggtct gatcgccggc attgttgccg
     5221 acgttcaagc cctggctgtc cttcggatcg aaggccagac ccggcgcaaa gaacgacgcc
     5281 cggctcgcct tcatcttgtt cttcagattc accttgatgc aatcgcccag attggcgcgc
     5341 agcgtgagcg gattcggcat cacgttgccc gcaacggtcg cggcttcttc ctccagcgcg
     5401 aagatcttgc cttccggcat ggtcatttcg atcttccgct cgaagtccac ctcgatcgca
     5461 tccggggcct tcggattaag cttcatcggg cgatccaacg ccacgacgtt gaagctcttc
     5521 accggagcat ccgacggaca gacggaattg ggagtcgccg gaatcccgag aggattggtc
     5581 cccctgggca gcggcttcaa gtccgccact tccttatcga gcacgcggat gatgccccat
     5641 ccaccttcgg cgaagtgcga cgttctaccg ttgaagtgga tgtagtcacc cggcataccc
     5701 tggaatcctc ccgccttggt gacgaggtcg taccgctcgg cgatcccgac gtgaatcgag
     5761 ttcttccggt tggcttccgg cgcataccgc tccgtaagga aggtatgtcc ggcgatcgtc
     5821 cagacgtggg attcgttcat cagctggtgc aacagacgga acaccatggt gtcgcccgtg
     5881 tacgcccgga gaagcggcgt gcccggatca ccgtggatcg cgctgctgaa cagcttggac
     5941 gtgtcaggat tgttggacag gcgttgtgca aacggagccg cgcggaagtt gaaaccgctg
     6001 ccggtcgtgt gcgtgccgcc gttgatgtac ttgttcggcg cattcaggat cttgtccggc
     6061 atctggaagg acaccgtctt tcctgcttcc aacgccactt cgatcggctg tccgggagga
     6121 ttgccggctt cgatgacgtt gacggtgtgc ggtaccgtgt cgtggatgga gaccatcaac
     6181 tcacggaagc tgccgctgac accggcaccg atcggctcgt tgctgtgaat gtccgcgatc
     6241 ggaccgctat acaccaactt gccgttcttc ggatcatgat aggtggatcc gaacggctcc
     6301 acgatcgtca cgccgaaccc gccatggggc caggtcgtcg caccgaacgc gtggtcatgc
     6361 cagaacacgg ttcccatatc cacgtccacc caccaccgat agcgcacaaa ctccggcgac
     6421 acgagatcgc ccgccgggtg atggtgcttc agtggcttga agaacgtgac catgtcgccc
     6481 ttgatttcct tgacacgggc gacttcctga cagctcttat cccgcggcag tgacgccgcc
     6541 ggatcgttgc ccttctccaa acaatccatg cccaccatca actcggtgtt gacgtggaac
     6601 gcggtggcgc cgggcgccat ctggatcttc acggaggaag cgccggcctt gacctcaccg
     6661 actagcttcg caaccatcgg agccggcaac ccatgcttgg tcttcttccc ccacatggtg
     6721 aacggccgga ccgacatttc gtactccatc ccggagatca caccgtccga attcccggtg
     6781 tcgaactgga agaagtgggg atggatgttg atcttggacg actggaagtt cgtgtaatcg
     6841 tcgtcgtccc attcgctggt caacaacaag tccacgcagt catacacgtt ggcgcggatg
     6901 acggcgggca gtttcagatc gtcattggct ctggtcaaac gctcttcttc gtgaaggacg
     6961 tagatcagac catccttgtc caccatggcc ggctctttcc cctgcttctt ggcaagggtg
     7021 atcggcatgc ggacaaagtg aatgttgtaa tgcttccgat tggcgttgtc agggcacagg
     7081 ctccaacggc cctgttcgcc tggcatcgcc ggctcggagg tccgctcacc gtttgcatcc
     7141 tggtgaatcg gctccaacca tggcgcaccg ctgtggttcg ccgagaacgg cacgcgcttg
     7201 ccgaaatgcg gcttgaacaa cggccaggcc attttccctg tcgtcggatc gaacaggatc
     7261 gccggtctgg tgctttcatc ccacttcgcg gcccacgcat acttcggatt cggagcggtg
     7321 ctttcccgct caccacgggc aatgttaccg tcccacttcc agtcccacac cgtcgaatcg
     7381 taggccatga tctggccctt ctcatcgttg gtgtggcccg gttgaccctg ggccggcacg
     7441 aacatctcga cccaatcctt gatgttcacg atcacaggat cggacttcca gttcgtcttc
     7501 tggctcttgt cgacgatctt gaaggtcttg ccgaaccagt cgacggtggt accgatcaac
     7561 ttatccgacg agacaccctg cttcatacgg cccaggcgat ccggcaactc gcgcatatcc
     7621 ggcatcgtgt cggtgtgcgc attgccgacc tgcagcgtgt tgtagaaccg gccgtacccc
     7681 cacatgcccg ccacatagtg gtgcgcaacg tggcagtgat agaggaactc gccggccagg
     7741 tgctgacagc caccgccgcc gcattccggt tccaggtcga gcgcttcgga cggaccgacc
     7801 acttccacgt ccacccgatc ggacttcgtc cggaccacgg gatacttcac cgggccgtcc
     7861 tgtcccttaa accaatgctc ttcctgatcg gcgcgcgggc tgcgctgcca acggatggtt
     7921 ccgctgtgcg gatggtgaga gtggaacact tccgatccac catgcaccaa acgccacttg
     7981 accggatcac cgaggtagcc acgggccacc gtggtgggcg ggtcaccgaa ggtgtaggcg
     8041 ctgtaggcca gcgactcatc ttcgaagccg aaatattcat gctgtaagtg catctgatcg
     8101 atgccgaacg gctcgctgcg atagttgatc gcacggccac ccggacgata ggcgtcggtc
     8161 aatggatcgc gctgcggcaa gaagtcaccc ttcttgttca gcggccggaa ggcttcgtcg
     8221 ccgatctcgt gatagaacaa cacgaactcg cggaagtccg gtccggaccc gttgtcgatc
     8281 atgacctgcc atccgctgtt cgtatcgggc gcggcgcccg ttccgagcgg atcgaggtac
     8341 ttggaaccct tcggttcgac gatgaaggcg ccgaagaggc ccaacaccgt caactcgcga
     8401 tcatggctcc atgagtggaa ctgccgcaca ccttcctgca tgttcggatg aatgtaccat
     8461 tcgaactcct gcgtcttgcc cggcgaaacc accgtgtccg ggttcgtcgt cgtcgcaggc
     8521 ttgcccgtgg cgctcacgat catgctggag gcctggatga ccaagctgcc atcctcaccc
     8581 tccatctgat tccgcaacgt catcttgacg caatcgccct ggttggcacg gagcaccagc
     8641 ggctgaatcg catcgccctg gtttccggtc gtcaccgcac caggatcgaa tccgtctttt
     8701 tcccgcgctt ctttgttctt ggtttcctcg gcgcgaacct tgtcgatctc cccggtatgg
     8761 acgtacatat acccgggata gaagtcgagc caccggttca aactgatttc gatgttgatc
     8821 atcgaaacgt cgtacttctt caccggcgca ttggcgggac atttcccccc tgacgtcatc
     8881 accggttcgc ccttcgacga atcggacgcc aacaggaagc tgctcccgtc ctgccccata
     8941 tactggtgca tcatcgacat ttcgttgaat gcaccggagg tctgctgcgc cttcgcgtcc
     9001 gtctgcgcct gctgttccaa cttctccatc aggcggtggt gctgcatctc catcttctct
     9061 gcattgccgg cgcgaccttc catggcgttt tccaccacgg tctgaccctt cagggtctgt
     9121 gtccagcccg gcatggcgac cggcgtcgca tgtccctcat gcgactgatc gcccggacca
     9181 gccgcaaaca cccacggcgc cgcgagcaaa ctgcccgaga gcagcgccgc ctgcaggctc
     9241 agcatcgcct tacgactgat gcgcctgcgc tttgactgca ctgaagacct catatgtcgg
     9301 cctccctcct ttataatccc gcgcccttcc gcgggagtgg ttaaaaaacg tggtcgtgtg
     9361 accggcctcc gtcaggcagg accccggctc ctccggcctc tcctttttcc ctccacgaaa
     9421 cgctcccaaa aggaaaaagg gctggtgaag gatgtcctca aagatcgagg gagcaggtca
     9481 ttcggaggcc ggtcctaatc acccgcacct tcaccagcac tgcctaacaa cgcaattgat
     9541 gtgcccacta ggggtagtcc tagccgtact gaaatgttct atatttttca aggagttgaa
     9601 gatttagccg ggaaattttg aaccggcgta aaatgccaca gttttccaga cgattagaca
     9661 gcacacctcg gcaaggataa gaacatataa aatacaatta cttagactgt ccaagccgct
     9721 ggacattaga tcgtgaattg tggggcgata cgggacagct tcctcgctta gcggttacga
     9781 aaggaagagc cattggactg attctgaaga agattaggat agagacaaga agcggggagg
     9841 gcctgaaaac aggctctctg ggctgagcta ctgaatgccg agttcgtgca gcttgttgta
     9901 gagactggcc cggctgattt taaggacacg agccgcttgc accctattcc cggaggtctg
     9961 ctgcaatgcc tgaaggatac gcacccgctc ggcctgtcgc gccgcgcccc ggccgaccgt
    10021 cctgaggtcg gtatcctgag ggtgactccc cagttcgacc agatccgcac aggtcagttc
    10081 cgaatgggtc gtggtcacca cggctcgctc gatataatgc tggagctcac ggacattccc
    10141 gggccaaggc gcagccgtca aggcacgcat ggcctcttca ttcacctgac gcaaatcctg
    10201 ccgatgccgc tggcaggagt cggcaataaa ttttacgacc aacaagggaa tgtccaccct
    10261 ccggtcacgg agcggcggca aatgaacggg caacacagct aaccgatagt agaggtcctg
    10321 acgaaatgcc ttggccttga ccaggtcagt cagatcctta ttggtggcgg agatgacacg
    10381 gacattgatc ttgaccggct gcgtcccgcc gactcgcttg atctcgcctt cttgcaagac
    10441 tcgaagaagc ttagcctgga aggttggaga cgtatcggct atctcatcca gaaagatcgt
    10501 gcccccgtcg gcctcttcaa acagcccgga cttggccccg gctgctccgg taaacgctcc
    10561 tttgacatgg ccgaacagtt cactctccag caacgtttcc gggagcgccc cgcaatcgac
    10621 caccacaaaa ggcttgtcgc gacgatagct cgtctggtga atggtccggg ccacgagttc
    10681 ctttcccgtg ccgctttcac cctggatcaa caccgtggcc gaactgtcgg ccaccaaccg
    10741 gatcatttca aacagctgat tcatggccgg actcgcgccg accagatcgc ccaatctcga
    10801 ttcggtccgt tccgggcgcg atcgaccgag cctttgctgc ggcagcgacg ctggaatgct
    10861 gagcggctcc ggccgcttgt gaaagagcac gaggatcgag gccacttctc ctgccgacga
    10921 gacgagcggg aaggcctgac gcatgccgca agccgtcccg tcgccggacg tggtgcagga
    10981 gaccgtgtgc acttcccccg tcttaaagac cgactcagca ggacaagtcc cgcaagggtc
    11041 tgtcatgtga acgaaagcct gataacactt ggccggcttg gcgaccgcag agggattccc
    11101 cgacttcgcc caagcggcgt cattggcata gaccacattg agatcccgat ccatcaccgc
    11161 cacgcggtcg gtcagcccgt cggccaagag cttcagagct tccaaccgag cggccagaat
    11221 cggatcggta aagggggaaa agggtgttcc ctgtgagtga gatccggcca tctcatcact
    11281 cctcgtttct atacgaacca agcggggcct ttctgatttt tatgaaggcc ctattagtac
    11341 tacagccacg atcacgaaga caaccctccc cccttggggg agacctcgcg aaaacacgta
    11401 tccactgcca atgttcagtg cagcagtcca tcctcggacc ggcttcctgg atgttgcagg
    11461 agttgcttga ccgtcgcctt cagttccgac agcctgaccg gcttgtcgaa gtaggccgtc
    11521 gcgccgtttt tcatcgcctc gtccttggtc tgggcatcgc caaaggcggt catgacgata
    11581 atcggacaac cgggcacaca gctcctcaat cgatggacat agtcgaaacc accagccggc
    11641 attttcaagt cggtcacgat cagatcggga gccgctcgca tgaccgccgc cagcccctct
    11701 tccccgttgc tggcctcgcg caattggtat ccctctcccc acaactcgtc gcagagcaag
    11761 ctccgcatat ccttgtcgtc ttccaccacc agtaataccg ctggtgttcg actttccacg
    11821 ttcgagccct cggaataggt caccatgggg acaacacaac gcaaaccatg tgccactcac
    11881 gtcggcgcgt gaaagcgagc aactgattgt attcaatgga taactagccc cgaaggtttt
    11941 tccaaacagt aggtgcatga accggcctct ggcgagaaag accccaccct ctgaaaaagc
    12001 cctgtcccga agcgccacaa cgggacgagt agagcttatg gcgaattgcc gatggcgtat
    12061 ggcacgagcg aagaaaagag gccgaccatt cccaaagact gttcaatccc tgccataagc
    12121 taaatgcaat acgctatcag ctcttgtcta tcggtaggca gatggtgaag gtcgtccccc
    12181 gctcaggctc actctccacc tcaatcgttc cgctgtgttc ctcaatgatg cccttcacca
    12241 cagtcaaacc taacccggtt cccttcccga actccttggt ggtgaaaaac gggtcgaaca
    12301 ccttggcgat cacgtcccgg ggcatcccgt ggccggtatc cgccaccgtc aaccggacca
    12361 tgccacggtc cggcgccagt gacagcttca taacccctcc cccgggcatc gcatggatcg
    12421 cattcatcac caggttgatc aagacctgac tcatctgatc ggaatcggca tgcaccaagg
    12481 ggcaggcgtc ggcaaaggag gtttccacgc tgatgttact ttgcgacagg cgttcctgga
    12541 aaatttccag gttgtcctcg atggtctggc gcaagtccaa ggcacggtgt tcgaccggac
    12601 gtcgccgggc aaacgccagc aactgattca tcacccgcgt aattcgttcg acctgactga
    12661 cgatggtttg gagccccttc ctgaccggct cctccttcgt gcgctccatc agatattcgg
    12721 cccggcccag aatgacgttc atgggtgtgc cgatctcatg cgccatgccg gatgccaccg
    12781 ttcccagttc ggccacgcgc tccgtccggc gaagttgcgc ctgcaggcgc ttccgctccg
    12841 tgatatcgct ggcaatgccg tcgatgcgca acggacggcc gtcggcatcc ttgatgaccc
    12901 gcccgcggtc gtgtagccaa cgcagatcgc cgtcgagcct gacgatgcga tactccacat
    12961 cgaactcccc cattgtcgag agggcctgtc gcgcctgttc cgcaatcacg caatcgtcgg
    13021 gatgaacggc ctccagccat aacgaaggcc tggctaaaaa cgcgtcgggc ggccggccat
    13081 agatctccat cacggacgga ctgacataaa acatcgtgga caggtccagc gacgacgacc
    13141 agatgacatc ctccatcgcc cccaataaac tttgaagccg ctcttcgctc tcccgcaact
    13201 tctgctcggc ggcttttcga agcgaaatgt cccgcaggac gaccaggatg ccgaacccct
    13261 cctcatcctg aaaccgcgca gccctgacag ccacgtccac actcatcccg tccaagcgca
    13321 cgatcttttc ctcgacctcg ggcaccgtgt tcccgggccc cagaagatgc cggatccgcg
    13381 catcgatcat ctcgtgatag tcgggatgcg ccaggtcata cagcgacttg ccgagaatct
    13441 cctcggccga tcgggccccg aacaaacgca ccccttgttc attggcgaaa aagatccggt
    13501 tctcgcgatt gaccaggatg gcatcgggca atacggccaa aagccgtcga taccgctcct
    13561 cgctttggcg caagagcgtt tcggacttct tccactcggt gatgtcgcga gtcactccca
    13621 agaccgccgt cacgatgccg tcggctgaac gcagaggcac catatgcgta tccagccacc
    13681 gcggcgttcc ccggagaccg accatctgaa actccagtcg ccccgcttcc ccccgcccgg
    13741 ccttgccgat caggtcacgg tagaccgacc gaaactcggc cgtggccagg tggcacacat
    13801 cccgcccgac gatgtctgca ctgctcgttg cttcgatcat ggccagccct gcggcgttga
    13861 tggttcgaac gctgccgtcc agatcaagca cctttacaca ttcgggctcg gtctcaataa
    13921 tcgcccgtaa cagggcctcg cgttccgcca actcctgctc gagccgtttg cgttcggtaa
    13981 tgtcgcggag gatgacggtg taataccgcg tcccttccac accgatctgc gagatcgcgg
    14041 cttcgatcgg aaattcctct ccgttggccc ggacccccat caccatgccc agcgcaccca
    14101 tttggcgcgt cgtgataccg gaacggccga actcccggat atgctcatca tgggtgctac
    14161 ggaaacgctc gggaagcagg cgatccagcg gccgccccac tacttcctcg gcgccccact
    14221 gaaacatctt ttccgccgcc tggttgaaca acacaatctt gtgcgcttcg tcgacagtaa
    14281 tgatcgcgtc catcgccgag tgcacgatgc cttcgaaccg aagtttgtcc tgatgcagtt
    14341 ctgcttcggc acgacgttgt ccggccaccg caccgaccac cgccgcttca gcccgggcac
    14401 gcgcttccgc gtccgaaacc tcgccggcct ctggttgatg ccgctcagcc cgcgccgtcg
    14461 tgaacgcctg gggcgacagg ccggggctcg cattcgtgct cgcaggagaa gagcgacggc
    14521 cggcgaccag cgcggccacg gcccccatga tcccgatgcc cagcagacga ttgagccatg
    14581 aggaaagcgg cagagacccg gcggaggacg caagagggcc gatgagcgcc agcaacgcca
    14641 ccagccccat caaatagcgg ggaacctgcc cgagtgaaga aaccgcgatc agagtgaacg
    14701 gaatcgcata gagcagccac aggtcgatcc cagcgggcgc cgacagatcc aggacgaaga
    14761 tcccgaccgt cagcagcaat gacacgccgt attcaaacag cggagatttc tgatgaaggg
    14821 gctgcacgcc gatggctccc agcgcgaggg tgagacggtg aatctacgcg tctgagtgtg
    14881 gtttgccttc gatttcggca agcttccgat acagggtctt ccgatcgata cccaggacgt
    14941 gcgcggcctg atacttgttg ccgcccgtct tttccaaaat tttcacgatg tactcttttt
    15001 ccacttcgtg cagtggcaga gtgcgttccg cggcttcatc gagaatccgg cgatcgcctc
    15061 tggagccttg cacctgcacc ggcaaatcgt ccgcgccgat catgtcccca tgactcagcg
    15121 tgacagcccg ctcaatgaca ttctccaact cccgcacatt ccccggccag gcatagtcgg
    15181 ccagcatggc aagggcggat tcgctgaggc ccttgacctg cttgcccctg ctgtcggcgc
    15241 acttcttcag gaaggcatcc accagaatcg gaatatcctc ccggcgttcc cgcagcgggg
    15301 gaaggcgcaa ctcgatcaca ttgaggcgat agtaaaaatc ttcgcggaat cgcttattct
    15361 tcacttcttc ggaaaggttc aagtttgtgg cggcgatgat ccgcacgtcc acgggaatag
    15421 atttggtcgc ccccacgcga cggatttccc gttcctgaat tgcgcgcagg agttttgcct
    15481 ggagcatgag cggaagttcg ctgatttcat ccagaaacaa cgtgcctttt tgcgcctctt
    15541 caaagagtcc gcgcttgtcc attttggcat ctgtaaacga gccgcgcatg tgcccgaaga
    15601 gttcactttc cagcaattgt tccgggatcg cggcacagtt caccgggaca aacggcgcat
    15661 cccgccggtc gctgttgtaa tgaatggcct tggcgaccag ttcttttccc gttccgctct
    15721 ctcccgtgat caacacgttg gtgggactgt cggccacgcg ccgaatcaga tcgaagaccg
    15781 cctgcatcgg tttgctcttg ccgagaatct gatgaaagct gtactccttg tgcacctctt
    15841 tccgtagccg gctgacttcg cgccggagcg ccgcttcccg aatcgcgcgc tcgaccaccc
    15901 gcaccagttc ctcggttttc accggtttgg tcagatagtc gctggcaccg tgccggatcg
    15961 cctcgaccgc cgtctccaca gatccgaagg ccgtcatcag gatcacgccg atatcgggat
    16021 gccggtgttt cacctccgcc aacaactccg tgcccaacat gcctttcatg cgaaggtcgg
    16081 tcaggaccac ggcatagtct tcctcactca gccgctgaag cgcgtcgtca ccactgccga
    16141 cacccgtgca ttgatgcccg cgaccctgca agacatcgca cagcaggctg cgcatgtcgg
    16201 catcatcgtc gaccaccagg accgcgcccc attcctcagt catacagctc cctcacgtgc
    16261 tcctctcatc ggaccagttc tctagcgagt gcgggcacag cctaatcggc ttactcccgg
    16321 gcggtcaagg ggcgtcctgc cgcaggccat tgccgcctgc tgtcgcgaaa tgccccaacg
    16381 tcactcacgg accattcttc gagccgccaa gcctatgagg tgagcacgcc acttcatcgg
    16441 gttgtacact catcccgcaa cggcacttct cttgcgtctt ccctggcgag acgggaggtc
    16501 gtctatggag tcagatcact cgaaacagga tccgcggccg accacccgtc cggcaccggt
    16561 cgtgctggct cccgttccgg tagtgcaagg tcagggccat gctcaggtgc ctgtcagagt
    16621 cggcgcctgc ccccttcctg gcagaaagcg acggacaccg cttctactcg cggcggtggg
    16681 gcttctggcg atcctggctc tcggattgtg gcattggtgg tccaatgacc cggttgccgg
    16741 tcactataag accctccccg tcgatcgcgg cccgatcacc tcactggtca cggccacggg
    16801 agccgtgaac cccgtcatct ccgtgcaggt cggcagtcag gtctccggaa agatcgcgaa
    16861 aatctacgcc gactttaact ccatcgtacg ggaaggtcag gtcctcgcct ccatcgacca
    16921 gaaaccctat caagccaagg tcagccaggc caaagccgca ctcaaaagcg ccaaagccac
    16981 attggccaag gcccgcaaca tgctcgcgca gaggacactg gagctcaatc gcatggctgc
    17041 gttgcgggag caacaattcg tcgcccagtc ggatctggat ctgtcgagaa ccaactatcg
    17101 cgatgccgaa gcacaggtgg atctgtctca ggcacaggtc gaccaagcca aggccaccct
    17161 gtcgtctgcg gaattggatt tgggttacac caccattatt tcgccggtca acggcaccgt
    17221 cgtctcccgt aacgtggagg aagggcagac ggtcgtcgcc agctttcaaa ccccgaccct
    17281 cttcgtgatc gcccaggacc tcacccgcat gcaggtcatc gccaatgtca gcgaatcgga
    17341 tatcggcggc gtcagcgagg gcacctccgc cgactttcgt gtggatgcgt acccgcgtga
    17401 gttttttcac ggggtcgtga cgcaggtccg gaacgctccg gtcagcatcc agaatgtggt
    17461 cacctacgac gtgatcatct ccgtcgaaaa ccccgaattg aagctcaaac ccggcatgac
    17521 ggccaacatc accatcgtga cggcgcgcaa cgaagatgcg ctccgcgccc ccaacgcggc
    17581 cttacgattt cgtatgcccg gcgtgccggt ggatcgcaag acggcgcagc tttgggtgct
    17641 ggagccaaca ggtcgtgtgc gggcggcacc gatcagcaca ggtattgccg actcgctgtt
    17701 cacagaaatt acgaccggcg aggcgcacga gggtgatgcc gccatcgtgg ggattacctc
    17761 agcggaagac agcgcccagg aaaaactccc gcccggcttt gagttcgggc cgaaaatgcg
    17821 gtgacgagcc tttcatgagt tttctctggc tgaccattca atccgccctg cgggtgctcc
    17881 ggcgtaatcc gctacgcgca ggattgacca tgctcggcat cgtcatcggc gtcggcgccg
    17941 tcgtcggcat ggtgagtctc ggacagggcg cgaccgcctc ggtccaacgc gagatcgcca
    18001 gcctcggcac caatgtgctc attatcatcc ccggcgccac cacgacgggc ggcgtacgcg
    18061 gtggactcgg ctcggtctcg acgcttaccg tggacgatgc acgcgacatc gaaaaacgca
    18121 tcggcgacat cagcctcgtc acctatgcct ctcgatcggt cctccaggtc attcacgaac
    18181 ataagaactg gaacaccatc gcgctcggca ccaccaccgc ctttcccgat cttcgcaact
    18241 ggcctgtagc cgacggcaat ttcttcacgc aggcggatga agacgccgcc gccaaagtcg
    18301 tggtgctcgg aaaaaccgtc gcggacaacc tgttcgagcg cggagaggaa gtcatcgggg
    18361 cgcagatccg ggtcaagaat gtgccgctgc gtgtgatcgg cattctttcc cccaaaggcc
    18421 aatccctctc cggacaggat caggacgaca tcgtcgtcct gccgttcacc accgctgagc
    18481 gaaaagtcct cggaacgaag ttcctcggaa ccgtcggcat catcatggtc gccacccagc
    18541 accgctacag catcccggct gccgtggagg agattaaaga actcttgcgt gcacgacacc
    18601 ggattcaccc ggccgaagag aacgacttta cgatccgcac catggaggat gtcgccaaga
    18661 ccgtggccgg agccagccgg accatgatgg tgatgctgct gagcattgcc tccatctcgc
    18721 tgatcgtcgg cggaatcggg atcatgaaca tcttattggt ctcggtgacg gagcgaacca
    18781 gggagatcgg catccgcatg gccgtcggcg ccaaacgcgc ccacatcctg ctgcagtttc
    18841 tggtggaagc gatcatcctg acggcgatcg gcggagtggc cggggtaata ttcgggatcg
    18901 ccggggcgcg gctcctgacc cgccttatcg gctggcccac aatcatctcg tcacaagccg
    18961 tcgctgtcgc cttcctcttc tctctcgccg tgggcatctt cttcggcctc tacccggcca
    19021 acaaagcctc gcggatgaat cccatcgacg cgctgcactg ggaataacgg aataggcggc
    19081 acaaatctag gaattcatca atgctcacgg tcactacgcc cgaatcttgc tggcctccgc
    19141 aagtatattt caaaccgagc aaattgtttc gggttttagc gagtggtaat attcgtcgtc
    19201 atttcattca gaccttgcat gaaaaaaact aaagaatacc ctcacacccg ctttcctcct
    19261 gaagttctaa ggaaggcccg tgagaagatt gagagctgga ctgaagaaca gtcaggcaac
    19321 agctccgcac tacaggcatc gattcagata gacgagcagg aaaaatggca gtttgacaac
    19381 gaagatgatt tcttcccgca ctatcgtaaa gaacatagtt actcctacta tgatgttacg
    19441 gtggggaaca tgcatctcac cattgaggtt tacagacata ctacgcgtat cgcggtacaa
    19501 gcaggagcac gatccaagat tgagtcagtc ttcagcattt ttgaagatgc cagttctacc
    19561 tccagatgtt tggatgagaa acagccccaa tctatttttt caaggcaacc aggattctct
    19621 accgatcgaa aactatcatc tcttaatgtc gacaagaatt tactcttgag acttgaggat
    19681 tacttcctgg aagagatccc aaggatcgca aagcttaaac cagaagaggc aagacgaaac
    19741 tacaagataa ctagcgctga ccatgctgga gtgcagcaat ttcgctcgat acgggaatat
    19801 tccggagatg tttttcctga taccatatcg agggtagtct taaacagcgg gttcattgca
    19861 tcagacgatg catcggtcaa cgtgaagctc tccctggaca ccgaacctca ctggtcttca
    19921 gttcaagtcg atgtcacatc cgctaataat ccgcgcgaac tttgccatgc agtcctacaa
    19981 ggattattgg atcgcctcga acctaataag aaccttcacc ggttattaca cccttcgttc
    20041 cccctaacag gaatcctata cgggttatca gcaccttccg gtatggcagc actcacattg
    20101 gcagggaaag gcttgtattt gccagcctgt ctggccgcag taccaccaac gattctgttt
    20161 ctactctgga tcggaggaaa agcaaaccca tacacagcct tcaaaacaca agccaatgac
    20221 cggtggtctg atctctggaa atggttctgc cttggacttc taggcttcct attatttgga
    20281 actggggcct tttatttgag gaaagcattg ttgggatatt gaccaaacca aggacggtct
    20341 gcctggaaat attcgcctgg catattcgaa gggcgaactg gtgggtcata agaacttctt
    20401 tcaggatgtt caaaaaggcc gtccggctcg gccgcagcgt ttcaatctac aacccggcgg
    20461 ctggggtaca ccgaactgcg cgcgtccaac gagggcctgc tgaggccgcg cgttgcgcga
    20521 gccacggagc accccagtcg cccaagccct tttcaacgtc ctgacacctg ttgttccgcg
    20581 tggtaagagc tgcggacgag cgggcccgat tcgacgtgac ggaatccgat ggcgaggcct
    20641 tcctctttca ggcctgcgaa ttcgtcgggg tggtagaacc gcgccacgga gagatgttcc
    20701 ttcgtcggtt gcagatactg gccgatggtc atgatgtcgc attcaaccga acgcagatca
    20761 cgcatgactt cgcgcgcctc atccgtcgtt tcgcccatgc cgacgattaa tcccgacttg
    20821 gttgtcatgc ccatctgctt ggcccggccc agcaattcaa tcgatcgctg atatttcccc
    20881 tgcggccgga tcgaggggaa caagcgccgc accgtttcga tgttgtgatt cagaatgtcc
    20941 ggcttttcgg ctgcgaccag ggcaagtgcg ccttcattgc cttcaaagtc cggaatgagc
    21001 acttcgatcg tgcaactggg aatcaatcgc cggatgtggc ggatggtttc ggcaaacacc
    21061 gacgcgccgc catcgtccag ttcatcccgg ttcaccgacg taatcacggc atgtctcaag
    21121 ttgagggcct ggaccgcttc cgccacgcgt aacggctctt cgcgatccac ctcatgcggc
    21181 cgaccggtgg cgaccgagca gtagtgacag cgcctcgtgc agatgtcgcc gaggatcaga
    21241 aaggtcgcgg tgcgggcgtt ccaacattcc cacatgttcg gacaacgcgc ctcttcacaa
    21301 atcgtgtgga ggttgagccg gtccatggtt ttgcgaatgt cgtgataatc cggaccggtc
    21361 tgaagcttca ccttgaacca ggacggcagt cggcgggatt gtggagctga cggctgattt
    21421 ccatcaacag tcggccgggg gtcgatgtga atgaagctca tggttttacc accggagcca
    21481 ccagttcctg cggatgttcc aacacacgtt tgaactcttt tagaaactgc gcacccatca
    21541 atccatccaa cgcccgatga tcgcacgaca aggtgacctt catccgccgc ccggcggtca
    21601 ctgttccctt cgtgacaatc ggtacatccc gaatcgcacc cacggcgatc gaagcagcct
    21661 gaggcggcat gagcagggca atgaagttgt ctacgtcgaa catgcccaga ttggagatgg
    21721 caaaggtcgc tcccgtatat tcgttcggct gcaggcgctt ctgcctggcc cgttcgatca
    21781 gcgccttggc ttctaccgaa atctcagcca gcgttttcgc gccgcagtct cgaaccaccg
    21841 gcgtaattag accatcctcc atgccgacgg ccaccccgat atcgatctgc tcgaaccgtc
    21901 tgatcgcgtc gccggcaaag gagatgttga tctccggatg ccgacgcaac gccagcgccg
    21961 atgctttgat gagcagatcg gtgatggacg gatgggtctg acggctctgc ttgaattgat
    22021 cgcggacccg ctccgcctgc tccatgtcga tttcgacggt cagataaaaa tgggggaccg
    22081 gcgccttgct ctgcaccgtc gccctggcga tggcttttcg catctggctc aacggctgat
    22141 ccgttccggc aagcaatgcc tgcgcaggct gagcggtcgc ctgcatcaca tcgtcttcga
    22201 caattcgccc gcccggaccg ctgccggtca gggcgctcag gtcgataccc cgttcagcag
    22261 ccagggcttt cgctcgcgga gaggcgaatg gacgcgcacc ctcaggacgc gccgctgcag
    22321 ttggggcagg ggccactccc ggcgcggcgc cggttttggc gccattgccg atcgacggcg
    22381 cggccgtcac accgtctgac aaggccgacg caatgtcttc atcagcctcg gcgatgaccg
    22441 cgatcaacgt gccggactgt acggtttcac catctcgcac gagaatcttg cgcaggatgc
    22501 cgggagcaaa ggcttccaga tccatgacgg ccttgtcggt ttcaatctcc gcgatcactt
    22561 cgcccgcgtg cacctgatca ccttcgcgct tcttccaggc cagcagcaca ccctcttcca
    22621 tcgtatccgt cagtttgggc atgaccacgc gactagccat ggatcctcct ccaggatgcg
    22681 gaccatcttc gtacaccggc tcgaatcaga cggcgaccgc ttctcatgct gcaccacaga
    22741 ctgatttgac ggcagcgacc acacgcggca catccggaat ggcggcgtct tccagcggac
    22801 ggctgtaggg catcggcacc tcttcgccgg tcacgcgaac gatggggccg tccaggtaat
    22861 cgaacgccgc cgtgtagatg ctgtcggcaa tctgcgcccc cagtccgcaa aagcgccagc
    22921 cttcttcgac gatcaccagg cgcccggtct ttttgaccga ggtgacgatc gtgtcgaggt
    22981 ccaaaggctt gagcgtgcgc gggtcgatca cctcgacatc gagcccgtca cgactcagct
    23041 gatccgctgc ctccatcgcc acgagcacca tcttggaata ggccacgacg gtcacatccg
    23101 ccccctcgcg cttcacctcg gcctgaccga gcggaatcgt gtactcccct tccgtcactt
    23161 cacccttcgt gccgtaaagc aattgcgcct cgataaagat cacgggattc tggtcgcgga
    23221 tcgcgctctt cagcaacccc ttcgcatccc gaggcgtcga cggcgcgact accttcaaac
    23281 ccggcacatg gcaaaaccag gcttccaggc tttgcgaatg ttgcgccccc agttgatgcg
    23341 ctgcgctgcc ggggccacga atcacgatgg gaacagatag ttggccaccc gacatgtagc
    23401 ggattttggc ggcgttgttc acgatctgat ccagcgccac gatgccgaaa ttcatggtca
    23461 tcagctcgac gatcggctgc aatcccgcca tagccgcgcc gatggccaag ccggtaaagc
    23521 ccgcttcggt aatcggcgta tcgaccaccc gctgcggccc gaactcctcg acgaatccct
    23581 tggtcacctt gaaggcgccc tggtaatagc ccacctcttc accgatcaaa aagatgcgcg
    23641 ggtcgcggcg catttcttcc cgcatggcct ggttgagggc ttcccggtag gacaacaaca
    23701 tcgtggtgct agtcctccac catcacgtcg cgatgcaagg ccgagggctc cgggaacggg
    23761 ctctcatcgg caaatttcac cgccgcggcc acaatgtccc cgacctcctg ctcaagcggt
    23821 ttgaagtcgg tttccacaca aagcgcctca tccaggatct gctgcttcag caacaccagc
    23881 ggatctcgct tgcgatgttc ctcgacttca tccttactcc ggtaatggcc atgcgacgga
    23941 tcggccatcg aatggcccat gaatcgatag gtcatcgcct cgatgaaaaa cgggccatgc
    24001 ccggcgcgaa cctgatccac caccgtacgc atcaatgccc gtaccgcgag cacatccatg
    24061 ccgtccacac gctcggccat aatgccgtag gaacgcgccg cctccgccac gttctcgtac
    24121 agcgccaccg cccgatgcac cggcgtgccc atcccatatc gattattttc acaaatgaag
    24181 atcacaggga gtttccacaa ggccgccaga ttgaaggctt catgcgcctg gccgctcgga
    24241 acggcgcctt cgccgaagaa acagacggtc accagatcct gcttctgata cttggtcgcg
    24301 aaagccaacc ctgtggccaa cggaatgtgt ccgccgacga tggcgtaacc gcccatgaac
    24361 cggtgggcga ggtctaccag atgcatagaa ccgcccttgc cctggcacaa cccggtcgct
    24421 ttgccgaaga gttcggccat caccttaccg ggatcagagc cgcgtgccaa acaatgtcca
    24481 tggtcgcgat aggcactgat cgcataatcg tccggcctca gcgccgcgat cgcaccgacg
    24541 gcaatcgcct cttcgccgat gtagagatgg agaaacccgg cgatcttggc gagcgcatac
    24601 atctccgccg atttttcttc aaaccggcgg atcagcaaca tctggcgata gagggaaagc
    24661 agatcgtccc tgtccatgtc ggtcattatg catgcccacg gcaagacgac tcaaccgtga
    24721 tgttcggagg tcgggactga gcaggatatg gagtgagacc gcggcggaga aacacaggtg
    24781 agagcacagt cgattccggt gcgggcgcga tgcccgcacc ggagcgagcc atctatttca
    24841 ctttggagaa gtcggcatca aactgcatgg tctgcccgtc ctgcaacgtg acgatcaccg
    24901 tcgtcttggc gttgggatca aacgactcgt atccgaaccg ggccgtgaag cgcgaccgat
    24961 aggccgggcc tgcgccctcg ttttttctgc cccgatcggc cgggctgatg tccaccggac
    25021 ggatattctt tcccttttgt tggaacacga tgtcggcctt ctctgcaaag tactcatcgt
    25081 cgccgcagag ctgcacttcc acttccatgt tcggcatgtc cacgactttc tgaatgaact
    25141 cctcgggcat gcgcacgtct ttcttctgct tcttcgattc cgctgcttcc tgccggccga
    25201 acgcttccag ccgataccgc ttggtgcgca agatggcgct cgcgccgcat tgatccttct
    25261 cgggatccgc tcccacccgc gtgaccatcg aggcctgctg caacaccttc ttcacgtcct
    25321 ccggggaatt ggccttttcc atcggtgccc gcccggcttc cagagccctc ttcgcctctt
    25381 cgggagacaa cttgatgtcg atcgcccacg ccggcccgcc gactgcccac accatcgctc
    25441 cgacgacaag cccgatgact ccatgggacc gccgacgccg accttcactg caataattcg
    25501 cgcgcatgag tacctcccta acgcgtcact gaacaagggc gcattgtaaa aggattgcga
    25561 agaccttttc aatctcacag ctgaacgggc aggaagcctg ctagcccgga ttattcacga
    25621 ataccgtcgc gaggcgttcg agacggaagc ccgccgaggc tgctcgcacg tgaggccgac
    25681 gcaggcgtac tagcagtccg tcgaggaggc tgaacgagaa cagcctcgcc gggcgagagg
    25741 atcggacgcc ggagcaggta ttcatgaata gtccgggcta gggattgaat cgagcccagt
    25801 tggggtaggg cttgcgggca tagagctgat cgacatcgac cgggcaggct atcccacatc
    25861 ggcgcagcat ctcggcgacc atgcgaaggg gcaatcccac ggccgccgtg taatcgccgt
    25921 cgatccgctc aatcagcgcg gcaccaccac cctgaataga ataagcgccc gccttgccca
    25981 gactgtcccc ggtccgcagg taggcagcca acgcctcttc attcaagggt ttcatcgtga
    26041 ccagcaccgt ggcggtctga acctggcacg actcaggctc aacgtccatc agcgcgacgg
    26101 cagtaaaaat cttgtgcgtc ttccccgccg tacgcctgag catggattcg gcatcggcca
    26161 gatcggctgg ctttcctagc acctccgaac caaggctgat cagggtgtcg ctgccaagaa
    26221 tgagcgcatc ggggcacagc tcgatgcaag accgaacctt gcccgtggca aattcaacgg
    26281 cctgctgctc ggcagaaata ttcggcatga cctgctcgac aaacgggggg gccacgacgt
    26341 cgaagcgcaa ccccaggagg gtcaacaatt cgtgccgtct cggcgaggtg gaggcgagaa
    26401 tcaatcgcat gggttacgcg acggagacgg aagaggtctg tgccgcaagc ggagtgggag
    26461 cagacgcgtc tcgctctgcc tgcatgagtt cctggcaaaa ctcggccacg atgcgttcga
    26521 catcctgcac gttggacgct ccgaacatct tgccccgcat cgccgcagca tggggaaatc
    26581 ctttgcagta ccagcccaga tgctttcgga cagaccggaa ccgttccagc cccgcaatcg
    26641 cttcatactg cctggcgtgg tcgagcatga cctgcatgcg atggctcact gagaccggcg
    26701 gctcccaagc cgtggcgggc aatgtggaca agtcatcccg ctcgctgaac gcccgccgcg
    26761 cctgctcttt ctcgcggaag aaccagggcg agcccaacgt accgcgcccg accaacacgc
    26821 cctgcacctt gctttcagcc gcgcgtcgaa tcgcatcgac caaggtattg agatcgccgt
    26881 ttcccagcat cagcgtgccg gtaccatggg ccagcttggc ggcttcggca atggcggacc
    26941 agtcagccgc cccccggtac atctgctgca acgtccggcc gtgcagggtg atggccgccg
    27001 gacgggccgc cagcaaatgt tcgacccaac gttcgaccac cacacaatca aaccccagtc
    27061 gcgttttgat ggacagcggt aggagccgac gcggaacgac cggcacaccg gaacggctat
    27121 ggttcagcgt atgaataaac tcaatgcggg agggtttaaa tccctgactg tgaatcgatt
    27181 gaccgccggc ccaatcttcc aaaccctgcc gggcggcgcg cacgatggca tgggccagat
    27241 cgggcgtctt gatcaacgct gcgccagatc ctgacgccgc gacactcttc gaaggacagc
    27301 ccatattgat atcgagcccg tcgaacccca gttcgcagac tacctgcgcc gcgcgataaa
    27361 actgatccgg gtccttgccg tagagctgcg caacaatcgg ccgttctgcc tcgctgtaaa
    27421 tcaacgagtc cagcagatgg tccggtccgc gacaaatctc attgacgttg gtgaactcag
    27481 tgaaggtgat gtccggtcgc ccctgcgagg ccaccacgcg ccggaaggcg gcatcggtta
    27541 ctccatccat cggcgccaat ccgagaatag gctgtggcag cgtcgcccag aaactcatgc
    27601 acgcacctca gccgattccg tcggttcgtt tgccgtgcga taggccatcc gcatttcctc
    27661 agcctcttcc atcacggtca cggccgccga gggacagtca tgcgcgacga attcgacgaa
    27721 ccgatcgatc ttcagcagga aaaaatcata atcttgctcg tcagtcctcg gccggctgaa
    27781 ccagaacgaa acgaaccgct ccaacgggag atgggctgct ataagtaagc tacaggtccg
    27841 cggaaacatg tccttcagct caccgcccca atgcagtagt tcgtggggca gataagtctt
    27901 tcgataccgg tcgaggttat cgagcgatcc accgagcaga gacggatgtt ccgatggcgg
    27961 caaggcctgg ccggttgtga cgaaccggac atagccctga tactgattgc gtagcgacgc
    28021 ctgctcatcc attgtcagag gctgccgcgt tccagtgcgt tgttccaccg ccggtcgcgt
    28081 gagcgattga gccgcgcccg cctgcaccac gctggtgacc attgattcaa atcggtcaca
    28141 aaaccggcgg aactctgtaa cgatggtagc gaccggcttc gtatcgacct ccagcgccac
    28201 acctttcaag caggtcaggc ggggatgtgc caagacctga gccagcagat cgaacaggac
    28261 ttccggaatc ggagcaccat gggcatcgat ccagtagggc aaggcctcag catcgaccat
    28321 gtactgggca ccgccctgcg ccgtgaattc tgccaagccg gcgacatgaa tttcgatcac
    28381 ccgttccatc gggaaggcgt ctaaaaattc agcggcaaag gcctccaacg tctgacgcag
    28441 ccaggc
//
LOCUS       DAORRQ010000055        26956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_111_length_26956_cov_78.889670, whole genome shotgun sequence.
ACCESSION   DAORRQ010000055 DAORRQ010000000
VERSION     DAORRQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 26956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26956
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111_length_26956_cov_78.889670"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1120..2472
                     /locus_tag="PK224_15460"
     CDS             1120..2472
                     /locus_tag="PK224_15460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRB17192.1"
                     /translation="MGLSYDPASQVTSILHQLTANSTQINKADYLYNGVGNRTSLTDR
                     RGSQTFGYDNLDRLTSASHPMLATPQSFAYDPVGNRTTNNSTVNAGNQLTADATHNYA
                     YDDNGNLTRKTLLATGNYTQYTYDAENKLTKVEEFAAGNPTAITTSSYRYDGLGRRIE
                     KVANGQTKRYVYDGEDILFEYDGSNLLEARYTHGPGIDEPIAVTKGSSTYFYHQDGLG
                     SVTDLTDSAGATAKSYAYDAYGNVLESPGTIDQPYTYTGRELDQETGLYYYRARYYDS
                     TTGRFLQKDPVGLKAGPNGYWYANANPVFFVDPFGLMSRAQCDELRARIFRKVGDLID
                     DLIRYDPVADGIGGHPTRGGKKTSPGGHHNEIQQRQRGIGKDLAKYAKECTKRCNDGP
                     PPPPLPEWVEEVATRPIPDVVYPVPQGPMLMGPVDSVPFPELPEFMVRPTFPRPLIIP
                     "
     gene            2492..3001
                     /locus_tag="PK224_15465"
     CDS             2492..3001
                     /locus_tag="PK224_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17193.1"
                     /translation="MSVDNEKESVRSLLRRAFLSWRGDSNSPALDHDGCLEMRAKLFT
                     VRPEDIEYFLPQVLEDLLDTHTNNAGDSQDAETVVDFLDVPTLELDAEFIREKWGRET
                     LAKFRSDAKNLRSAKQAALAGVTRDQAQAICQWLKYARTWGDLEWNMDSVESALSYWS
                     KRVASHLSV"
     gene            2998..3906
                     /locus_tag="PK224_15470"
     CDS             2998..3906
                     /locus_tag="PK224_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17194.1"
                     /translation="MNPRLSTTFAYDGRNRLLSTTDPLGKIETYTYDGNDNLLTRITP
                     KNETISFAYDAVNQLLSKTLPGSQVTSYLYDQVGNLTTVTDPDSVLAMTYDQANRLTT
                     VKTDGSPNQPAVTLAYSYDPTGNRLSLTDPVQVAQYHYDALNRLQSLTQPSTPATQLP
                     NLLAAWPGDGSAADPVGGQSGTLQLGTTFRPGVRQQAFAFDGVDDYVNIPDSPVLDNL
                     STTATLEAWIKPEVPVGAEAWLFARRDPFVSEGFSVALLQDGKIQLTVRTTTSPTGSV
                     SRPPVRRLVLYHVVRIFLVNESRARA"
     gene            5514..5822
                     /locus_tag="PK224_15475"
     CDS             5514..5822
                     /locus_tag="PK224_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB17195.1"
                     /translation="MKKTWLTIDELALELQVSVKSIRRAYRKGQIPVERICRFVRFDL
                     DRVKAAMQGNEQGQSPFSTGSQGQRSATGGGSRRRAQRPRPRLGKTGASIAQAPRRKK
                     "
     gene            5819..6823
                     /locus_tag="PK224_15480"
     CDS             5819..6823
                     /locus_tag="PK224_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HRB17196.1"
                     /translation="MTVSGGFTQTIDWLAFTLPKAEVADVITLIGGDWFQSETGFRGY
                     PVAQLMTEGKTGVGKLGTGAPRNPKEVHVDLSAGIVSQWDETKLKTVLAWIFAQKGHV
                     TRIDVALDDREATVAVETVRQAVEAGQVVSRSKQFKVIQASNHRQGVRTGETLYFGSR
                     ESQSMLRVYDKRLELKSRGREDAESYGVRWEMEFKQDRAQACAKALLTLDAEDWRAFL
                     VGVLRSYVDFRETTREAESYEKYRAPLLAWWEGLTEGFRRCRLVVERIQQRLDDVVAW
                     FANALSPMLAVVVACRGDQFLTEMIYAGTKRWNQKHYALLKQRKKVVTPYVLHLKPRT
                     "
     gene            7030..7293
                     /locus_tag="PK224_15485"
     CDS             7030..7293
                     /locus_tag="PK224_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17197.1"
                     /translation="MQVKAEGAVQGYVERRSREGKVYRSVDFYVKGKDPGVLRLGIPE
                     DQMPLIDACKQAEGKQARASIEVRKFEQTGRTFFDLTGLDVQK"
     gene            7385..8542
                     /locus_tag="PK224_15490"
     CDS             7385..8542
                     /locus_tag="PK224_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17198.1"
                     /translation="MKLFTLTSYLSLIWGLLVSFCVAPGESLAQTATQYSRVVAQAER
                     IAYLAAQRSALASQVATAALAPSATSLAVRMVAGPVGWAALGVSAGLVLAQMYYSQPD
                     LAAIKQAASPPTGNWTYSYNGQTYIIPNHNIAGSPTPAANCPGGLMFGYLGSPTPGMT
                     SAGPTNWWCPPSPAQQTTGTPTASDIQQYLLTQSPTDPNAIEQHTSPVGTTGTTQPAD
                     NTVSQPVSPTEMPTTVKPKPVPAGDIVVADNVPPPASTPQQNTQQQTTTTTTTTTQNP
                     DGSTTQEEETQATTSCSVGSHESRTFGTVLQGHQARWGTSGLLGTLNLLKSLTWPSTL
                     PVIALPSAFFGSQQVDFNQWAWFFTVLRTLVIATASIVAYRIIFVGGGQTA"
     gene            8539..8814
                     /locus_tag="PK224_15495"
     CDS             8539..8814
                     /locus_tag="PK224_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17199.1"
                     /translation="MTAILTLIYCWLQEFFFSLTDWGLGIWDSLLSVADSTLAAIGTA
                     GLTLPVIPDQYAWVLGATGMSQALAILASAMGTRFILQTIPFVRWGS"
     gene            8894..9901
                     /locus_tag="PK224_15500"
     CDS             8894..9901
                     /locus_tag="PK224_15500"
                     /inference="COORDINATES: protein motif:HMM:NF017518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HRB17200.1"
                     /translation="MIELYEGVPGSGKSYHAICEKFLPWVKQGRRLYIAVDGIYLDRL
                     SLFTGIDLETLEQQITIWKDSVEVLQAFPHVEPGSAVIIDEAQTVFRSMQKVEPGLLR
                     WLETHRHYGVDILLMSQDFRQMSQGVTRLIEATVKFRKLAFVGLSKKYQGKVRGNPED
                     NEVIRAFVGSYSPAIYAYYSSYASAAIREEKRSHAVFKSARVAIGIAAGLFAIGLIFW
                     RPWSSLSSTKGTPTSAVSGTSSLPPLPSASAPGSLLNPFGASVPSTASPQFPKRTVRI
                     LGGAGSSANRQGWRYLLDSGEILTAAQITGRYGLLVSEVYEDGSMRLIGEGVFYGPAG
                     D"
     gene            10062..10274
                     /locus_tag="PK224_15505"
     CDS             10062..10274
                     /locus_tag="PK224_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17201.1"
                     /translation="MKGWSWVKGLGGVLMAFVFALGVPALSQAQLFPVSADVATVRAD
                     LLLWATALIGVALAIYAFKRVRAIVG"
     gene            10341..10469
                     /locus_tag="PK224_15510"
     CDS             10341..10469
                     /locus_tag="PK224_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17202.1"
                     /translation="MTAQQIQTIGNALAADLVAWGTAAIGLALVAAGAAWVLRLLR"
     gene            complement(10466..10969)
                     /locus_tag="PK224_15515"
     CDS             complement(10466..10969)
                     /locus_tag="PK224_15515"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRB17203.1"
                     /translation="MSVDSSLGSSNGGRPLKPVRKYGIPRYRVTLVREGRAIPAAESV
                     HTSEGAAAILRPLFAGLDREQFLICGLDAKHGLIGINVVSTGSLNLTIVHPREVFKPL
                     ILMNAGAWICAHNHPSNDITPSLEDRALTKRLREAADLFGIPLLDHLILAEERYYSFA
                     DQGWPGA"
     gene            11558..13135
                     /locus_tag="PK224_15520"
     CDS             11558..13135
                     /locus_tag="PK224_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB17204.1"
                     /translation="MNIRRILASRKIRSVLTYWYRGVRYRPVLGYNLSIDQEREAALQ
                     IITAIHANTAQPVRSASQPIQFADQSDTTFSAFIPIYLQYLKAKRPTNDGRNEVILTK
                     HIIPYFGQKRLAEIRLEDGLAYLEKRRSDVTGPRDNRRPVAAGTIERECAVLMAVLNL
                     AVDMDRLDKNRLKRLPVPEYVKRERVLEGWELLKLKAAASPQVWRLILAALQIGLREN
                     KLIETHEEWLMQRGDGWWMAPAPGRTKIKGVPKTIPLNRLAYEALFGGLSRIGGRFFG
                     QWKDGNSFKHSWMRTCERAGIHGLHFHDLRHTFATWLMQCGVDYAVIQTLKGEPLPGS
                     AKYYIHNWDGRLRDAVTRLESFTEALLNGENASEVPLTATQVPPSYVEQSLSPRFMVP
                     RDRIELSTPAFSGHALCLQRIETISDLSSRCTWTTRWTTDHFRLHSEDPPLTPIRDVG
                     RGVHFDTFSRGEHNFYLFDVAEPCYWPMSLIVGQAVRECPCSSAQIVKIRLDHELVGE
                     SNGTVQSLSRSDLEPGS"
     gene            13156..14220
                     /locus_tag="PK224_15525"
     CDS             13156..14220
                     /locus_tag="PK224_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17205.1"
                     /translation="MMICLFRNQLGITLLTLVLLFSGCASKPPQELSAACPDVAKTGQ
                     LNVLTLNTLYDAPAAVRTKSWADIAQFAVANNVHVLLLQEALLTDVDQIQQLLGTSDS
                     ARDLQRILNARSPEPYELRVAWETGVPLVLTTANAILSRCDVTRHFSTFLPIESEEAF
                     EGIELKITRNVQVAQMSIPGYGNLHIYNTHLCAACSIEGLQQQVAALLAFVQRVEAKV
                     QGNHVVLGGDLNLDLAKGVADQAVYETVTRAGFRDVYAEYRRTQFGETRQTLCWNGVP
                     DIHCTDGVSPVQGMIDKQTGAGFPKPTRLDYLFLRGTGTVQTSRVIFNPGNATTGPIN
                     PAEPAVSDHSGVFARIMLGP"
     gene            14826..15863
                     /locus_tag="PK224_15530"
     CDS             14826..15863
                     /locus_tag="PK224_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRB17206.1"
                     /translation="MLTNLSIKISIGLFLSALIFVLPTVQPSQAGVEGGTRGVEVVLK
                     EPEVILRSLYDKSWAIVIGINHYPGGDRNTPNLNYAVSDAKRVAQKLAGLGFEVRTLI
                     DEQATRQNIIHLLADDIGPQTKENDRIVFYFAGHGATKEKADKTYMGYILPHDYNPSK
                     HSATAISLQQLREASSEIKAKHMLYLMDSCFSGGLLAGRGRMPTAGTAGYQYLLNITK
                     GRSHVVIAAGGKDQTVKEEGGGGVFTRVVLDALSQRSNMPWAKDGYVTAMDLASYVMK
                     RVPDLAPQQTPQYGHLEGEGDVVVDIFQPLDVSGGDERAHKIFEAEQALKAEEALQRA
                     KRKRNIVAPGF"
     gene            15920..16813
                     /locus_tag="PK224_15535"
     CDS             15920..16813
                     /locus_tag="PK224_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17207.1"
                     /translation="MKGIVFSFVVILTTFTFNPVFARDPGGPEEPEASEKPLFSLKTD
                     DREPLFGDRFTLKAGYKIWVAKWQAQFSATTTAQNQVNTDHPMALNGPTVTGLLKLRD
                     SSWFNAAFFNFVWLHDGADFAEQRDDSSHIFANRRDYSFTAGLAVWEGFGVFGGYYNS
                     RQEFTNQPNLPGLGVSRHPRLVEGPIVGVFGNAQASEHIGLYGNLALAMLSFSGFCPS
                     PNSCARTDSAQGYMNEVGVTIQGPRVWRIGTELQLGFRAQIIQKNFGINATGAGTPNA
                     SNQQPLLDVTYGPVFTIHAVF"
     gene            16844..19423
                     /locus_tag="PK224_15540"
     CDS             16844..19423
                     /locus_tag="PK224_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17208.1"
                     /translation="MRAIRMMVVLVALSSLLSGCQRLCHVLCDSPLNPEGQLAVFSGQ
                     VLSTNNVPLTEASVTVNGRAMTTDDKGFFTLTVDSSSRYLVTIRKRGYSLFSRIYTKG
                     ILNKNWRLTRATLTTLDPAAGGIVQDTLAANCLANRSRSGWTHLEVKGNPVSPALRTA
                     LDGAYQPAGCSAGVSVDIPADALVDANGNRPPGRVEVEVATVDVLSPEAMPGDWGAQF
                     SMPREGGERLGFMQTFGAGSVSVTAGGKSYQLKPKTTARLTIPVDPTAAQIYRRMEQP
                     LPSSIPLLLYDEKQGIWKQMSIAQLNESRDAYVAELPHFSEWNMDVVFSDVACTQIDS
                     RLISGGYYLKAVPSVIPAGGLQPHEFDIPNTSGCLGNTTGDPFYCYIHAAWRLPSSPA
                     HGSGQVSFMPGTRTGPNSGDIVYNRANFVVPFGPAASNQQQMPPSYSQHPTQPALRPG
                     SYPNCTTAVTFADKPTLQVTSTATALTFTPSGYWGPNSTIVPNHPDDRYEFQWCNIAA
                     NPSCSSNPDDSGWINFASLPSISITGAAVRPARAVTSSPIQKSSLPAGTYKFRARAHM
                     GDSSFLIGGSETPWSDPSITITIGGNSPPTLTSIANQTVNVGTLVALTANGTDPDGNA
                     LTYSLTTAPSGAVINASTGGFTWTPLGAQGALTHKVTVLVTDNGTPNLSASTSFTVTV
                     NPQVLIENRLGGGPGTGCTSPPIDTSVIRLQVEGVPKLTPLSDDAVAGGNDPSYPNEG
                     IYITAGSDSLTFNPTATTYTVVIELGSWTGGGSGAGGPFVQRRYTNHGAAPETCFGLP
                     SYRYMDVTVNQGPGPLRLRIEPTGNQYQVIVQSGTATVNTPAAGQKSITDFTRTAGPY
                     TTP"
     gene            19575..20519
                     /locus_tag="PK224_15545"
     CDS             19575..20519
                     /locus_tag="PK224_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HRB17209.1"
                     /translation="MADWIVKLSMTGFLLWVMTSGIAAGAEVKIENRVGSGSTNGCNT
                     RRDTSIIRLRVEGVHKLLPLHDDVGAGGNDPSYPAEGVYIAAGSDSTVFNSSATTYRV
                     VIELGSWTGGGSGANGQFTQARFTHDNATIGGCDNPRYRYLDFTVTGQTPGLLTLRLQ
                     PMGNQYQAVVQGGMATLSTAATGQKTTTDFERTTEYIPFNEKPVMNAQRFSVAENSTA
                     GTVVGTLVATDPDGGPLTYSVTGGTGQGVFAVNPTSGQITVANSSRLDFETEPSFALQ
                     VQVSDKGTPQLSASATITIELTNVDEGAPAAPKNLRIR"
     gene            complement(20600..21607)
                     /locus_tag="PK224_15550"
     CDS             complement(20600..21607)
                     /locus_tag="PK224_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRB17210.1"
                     /translation="MRNCVETKDDESMAVRRPALPAMTPRWSRILLAVATTVMGWALP
                     GQSVLATPPLETVSGTGCYSYGDYQTPALAKRAALALAQEQAVRTHRVFVQSTSTVKN
                     LRLEEDIVTTASSGMLEQIHIDKQEQKGQEICISITAKISPVKFDDVIQQKANAKQIA
                     DAAQIPVLTEGSSFDLQLWTNKPAGEVYTEGEELEISVRSNRDAFLKIDYYQADGTVV
                     HLVPNMFVDDAFVRAGKTYTFGGRHARSGFRITGPFGAETIKAVASTRPLDSLLASEK
                     PVEESQSYLKNFHAQMRGVKVVANPDQPAQWAEAAFGLTTVSKSALEQSRFLSGIRGN
                     Q"
     gene            complement(21929..22243)
                     /locus_tag="PK224_15555"
     CDS             complement(21929..22243)
                     /locus_tag="PK224_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17211.1"
                     /translation="MSVASYTNMTEAIQELRKRGFTANFEFLDQEFRDVDSEKIFTAD
                     ELSIVEHYRFEGASDPEDMSVVYAIESHDGTRGIIADAFGTYANPELGGFLNNVLLRE
                     NQ"
     gene            complement(22396..22902)
                     /locus_tag="PK224_15560"
     CDS             complement(22396..22902)
                     /locus_tag="PK224_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17212.1"
                     /translation="MKPTLSLFLTIAMMALVVAPAGAEQATPFVQSAHYGFSGVITKI
                     ESGMLFVKTDTSLQARVISPTKAERVGLYDAKIGETVNLLVDSGNVLMDVSRTDQFFP
                     EHHFVAGRLHYTDPLWSAIKLSTPEGPASFDVDPLAGSKLSTLQEGTPVTIELDADNV
                     MVDIHWGR"
     gene            23550..26504
                     /locus_tag="PK224_15565"
     CDS             23550..26504
                     /locus_tag="PK224_15565"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HRB17213.1"
                     /translation="MSAGCGLPEISPFIGWEVLEIKGNKGVMLNGSSARKKIPIVPNH
                     SSWRTLSLSFTLSLLPLLWVPVSEGQTTSITSSGLGTTISPASIDPAGRPNYIITGGT
                     RPGDGPNLFHSFGDFSVGTNNVARFFNEAGRSTTNILSRVTGGQTSNIYGTIQTAGFG
                     TAALWLINPAGIVFGPSASLNVGGSVHFSTADYLRLGSGNDRFYADLGKTSQLTSAPV
                     TAFGFLGERPAGPVTVQAGSPFAVGEGKTISLIGGDISVTGRTLSAPGGQINLASVAG
                     GGEVVLNQVGQTPSLDLVNVSNQGTIQLTEGAILRTSSATSDAGAIFIRSGQFFMENA
                     GLEAISTAPFDAFNPTPTGGAVDILSEVMSIKNGALDLPGAGSLPQGQLGFAGHIPGV
                     SVLTNNSGDITLGTSTLSLNNAKILSYGQHIDSQFIAPDRFTGYIHIQGTGGAGSFAD
                     RVAIENSSLLHTAGFASDCNCTVFARDVRIQSNTVDMANSRISTNEGIIAISASNQIL
                     SHGNNTIAKEFGQRDLRGGENAIELTAGDVIVLGKGDTISTGAQGSSFTGGGSRAGDI
                     VLNAPSITMNGTTLKADGNFTVNGGSIILRSGAIELSESQLSTNGLDGGIQPFSGIAG
                     DILLSGRQAGVQTNTIHLTNSSLESAVLKSSQSSTAFGDSGNITLEGQHVTLDHSSVS
                     VAHEGVGKSGFIMLSGGQVDFVNNSELISAASGKSIPFSSEFSRSAAAPGGDIAIQAM
                     DISLAQGSRTKASSSGDGNAGAINLISIDSISIANSTVSTSATQASGGNIDLTAPNLV
                     RLVSANVTSSVNGPANSNGGNITIDTAHPQFVIMQGNSQILAKANEGQGGAITIIGGV
                     VLQEPGSVLDATAGPAGISGSINIQAPFQQLAGAIAPLPQAFAVAASLYGQRCATEKG
                     GQFSSFVQGARDGVPPQPGDLIPSPLMLELDEAAFSRGSQSIPSLAAIRLGLPEFEQT
                     SHSLTVFAGCRS"
BASE COUNT         5817 a         7366 c         7808 g         5965 t
ORIGIN      
        1 ctcctgtcgt gccatgatgc aaaacctcct ctatagatac aaggtcttgc aatcacacat
       61 acgcgctcgc gtcgggacac tgagtcaatg gtcccgcaag tctacctcgt tgtgaaccct
      121 gccgtgattt cttcttgcgt tcactgccgc aatcggtaga attcgccatc ctgtcactcg
      181 gcatcagtct cggccaggaa ggggaatgtg tgattgcaag acctgaccct ctttgccttg
      241 cgcgtgagta ttgtgagaat cctaagcaaa acccgtgcat acggtctttg gcctcagcag
      301 gaacaatcag cgttattcgt caagagctca tcattgaaga tcaaaaagcg cgcatcgatc
      361 gctcaacgga ggcatgtgac aggccgggct gtgcaacagc tgaggccgtt actgcgtatc
      421 acaagaaagt gtttgccgcg catggtatcc ctgagtggtg cttcccgtct tggtggttgt
      481 cagcaagagg gacgggagat cggtgagaaa gccgacaacg actcatctcg tggcatgggt
      541 gtggctactc cttgctgtcg ccttcgcagc acaggcaggg cgttatacat acctgacatt
      601 ttatcaggga ggtcccgatg aactcttgcg cagcaagatt ataggtttgg cagacggaat
      661 tcctcttgcg ctgctctgcc tcagcaattt ctttttgcag agaaagacgg cacttgaaag
      721 tctagttata cagattctca tgactgtaac ggttggtctc ggactgtgct ttttgggggt
      781 aggcctcttt gacatttttg attcgtggcc agatcctgaa aaatcattcc agcctagctc
      841 tcttctactg ataacactcg gttttgtcgg ctacccgctc ggggttgtgc tagttgccca
      901 tcgctactgg agacgtagcg ccacaatgac gcttcctgga gtgacgcttc tgccattttc
      961 gatctgcatc ggtctccata tcttggaatt tcttgtgtgg tggttttggc cattcgggtc
     1021 ggcttctcta tatggatcag cctcactcgc tctctttttt gctgcgacaa atttctcgct
     1081 cctaacaagt cctactgacc tacgaccatg agatccaaag tgggcttatc atacgatccc
     1141 gcttcgcagg tcaccagcat cctgcatcaa ttgaccgcga attcgactca aatcaacaag
     1201 gccgactatc tctacaacgg ggtggggaat cgcacgagtc tgacggaccg gaggggaagt
     1261 cagacgttcg gctacgacaa cctcgatcgc ttgacgagcg cctcccatcc catgttagcg
     1321 acgccgcaga gctttgcata cgatccggtg ggcaatcgca cgacgaacaa cagcaccgtc
     1381 aacgccggga accagctcac ggcggacgcc acgcataact atgcgtatga tgacaatggc
     1441 aacctcacgc ggaagaccct gctggccacc ggcaattaca cacaatacac gtacgacgcc
     1501 gaaaacaaat tgaccaaggt cgaggagttt gcggcgggga atcccacggc gatcacgacg
     1561 agcagctatc gctatgacgg cttgggtcgt cgaattgaaa aggtcgccaa tggccagacc
     1621 aaacggtatg tctacgacgg ggaggatatt ctcttcgaat atgacggcag caatctgctc
     1681 gaagcgcgat atacgcacgg gcctgggatt gacgaaccga ttgccgtcac caaaggcagc
     1741 tccacgtact tctatcatca agatggtctc gggagcgtca ccgatctgac cgacagtgct
     1801 ggcgcgacgg ctaaaagcta tgcctacgat gcgtacggaa atgtcctcga atcgccgggc
     1861 acgattgacc agccgtatac gtatacgggg cgagagctcg atcaagagac ggggctgtat
     1921 tactatcggg cgagatacta cgattcgacg acggggaggt ttttgcagaa ggatccagtg
     1981 ggattgaaag ctggccccaa tgggtattgg tatgccaatg cgaatccagt gttctttgta
     2041 gacccatttg ggctgatgag cagggctcaa tgtgatgagc ttcgggctcg aatttttcgg
     2101 aaggttggag atctcatcga tgacttaatc aggtatgacc cagtcgcgga tgggattggc
     2161 ggtcatccaa cccgcggcgg taagaaaacg tcaccaggtg gtcatcacaa cgagatccag
     2221 cagagacaaa gagggatcgg taaagacctt gcgaagtacg caaaggaatg caccaaacgt
     2281 tgcaatgatg ggcctccgcc tccgcctctt ccggagtggg ttgaagaggt tgccaccaga
     2341 cccattcccg atgtggtgta tccggtccca cagggcccga tgctcatggg gccggttgat
     2401 tccgtaccat ttcctgagct gcctgagttc atggtgcgac caacgttccc gcggcctcta
     2461 ataatacctt gatgcgcgag ggagctgaag gatgagcgtt gataacgaga aggagtctgt
     2521 aagaagcttg ctacgacgtg cttttttgtc ttggcgtggt gattcgaact ctccggcact
     2581 tgatcatgac ggatgccttg aaatgagggc aaagcttttt acagttaggc ccgaagatat
     2641 tgagtacttt cttccgcaag tcttagaaga tctgttggac actcatacaa acaatgcggg
     2701 cgattcgcaa gatgctgaga ctgtcgtaga ttttcttgat gttcctactc tagaacttga
     2761 tgcagaattt attcgagaga aatggggcag agagaccctc gccaaatttc gaagtgatgc
     2821 gaagaatctt cgctctgcaa agcaggctgc gcttgccggt gtgactcgtg atcaagctca
     2881 ggccatctgc caatggctca agtatgctcg aacttggggc gatctggagt ggaacatgga
     2941 ctcggtcgag tccgctctgt cctattggtc aaaacgcgtt gcgtcacatt tgtccgtgtg
     3001 aatccacggc tcagcaccac ctttgcctac gatggccgca accggctgct gagcaccacc
     3061 gatcccctcg gcaagatcga aacctatacc tatgacggca acgacaatct gctcacgaga
     3121 atcacgccca agaacgagac catcagcttc gcctacgatg ccgtgaacca attgctcagt
     3181 aagaccctgc cgggcagcca ggtcacgagc tacctctatg accaagtcgg caacttgacc
     3241 actgtgacgg atccggatag cgtgctggcc atgacgtatg accaggccaa tcgtctgacc
     3301 acggtcaaga ccgacggctc cccgaatcaa cccgccgtca ccctggccta cagctatgac
     3361 cccaccggaa accggctgag tttgaccgat ccggtccagg tggcgcagta ccactacgat
     3421 gccttgaatc gtctgcagtc cttgacacaa cccagcacgc ccgcgaccca gttgccgaat
     3481 ctgctggccg cttggcctgg tgacggcagc gcggccgatc cggttggtgg gcagagcggg
     3541 acgctgcaac tcggcacgac cttcaggccg ggggtgcgac aacaggcctt tgccttcgat
     3601 ggcgtggatg actatgtgaa cattccggat tcgcccgtcc tcgataacct cagcaccact
     3661 gccacgctgg aggcctggat caagccggag gtccccgtgg gagccgaagc ctggctcttc
     3721 gcaagacgag atccgtttgt cagcgaaggg ttctcggtgg cgcttttgca ggatggcaaa
     3781 atccaactga ccgtgcggac gaccaccagt cccaccggct cggtgtcccg tccgcctgtc
     3841 cgccgtctgg ttttgtacca cgtggtgcgg attttccttg tcaacgagtc tcgtgcccgt
     3901 gcgtgaacga gattattctc tcgtttgctg acagattgtt cgtggcttcg acatgcttgg
     3961 cggctcgagg cgcctctgcc gacagacagc aggcacactc caccggacac tctcagttca
     4021 ctccattgtg ttctgctctg ttctcgtcgc ctagctcgaa cgtgctttca acacatcgat
     4081 tcccgcccag acttcagctg gcgttcggcc ctgcagatgg tcgtgcgggc ggtcgtggtt
     4141 gtaccacagc cgtatgctgg tgagtttcgc ctcgaagtcc ttgtcgcttg tcagcggttc
     4201 ttccttcagc cctcgcttga ccgtcccaat aaaccgttcg accgcggccg ttctgccacg
     4261 ggcagccggg ttccgttcgc tgatgacgga tacccagcag gcgtagtccc cctgtgaacc
     4321 accctgatgt gaacaccgcc tcgttgtccg tgcggaggta ttgaggcctc ccgtactgct
     4381 tcacagcctg actcagttcc tgcagcagcc tccacgatga cttctccgag agccgctgca
     4441 atcgtaggca ggcgcgggtg gcatggtcca cgatggccaa tccaagatgc atggtaccct
     4501 gctgatctgc cctcgtcagc aaatcgcagg cccagacacg attgcgcggc atcggttgcg
     4561 ggacacgatg tttgagcttc cggcgtgctt cgaggacgag gtactggtgc ttccgcagcg
     4621 tcatcgccac atagctttta cctaccgtta cctgtcttct gcttgcccag cggcgattga
     4681 agtgatgggc tacggtgcgg cagccggcgt ggggcatgag ggctttgaat cggatcactt
     4741 cattgcgcac ccatgtcggc ttcggtggcg aaaaaacctg gcctttcgcc ttgggttgga
     4801 ctcgggctcg ctggtgggat cgtgcgggag accataccca ccatagtagc cagcggaatc
     4861 tgtgaaggca gcggtacagc attcgaatca gccagtgtcg catggggggc ctcctcaagc
     4921 gtgaacgtgc gcccgtgtta tcgaggactg aggaggggcg gggcacatcg tgtttggcga
     4981 tggtgaaact tccgtgttcg tgtgcgatcc ggtgctgtgg tttcttggag cgtcgtgcga
     5041 tgccccggct acacagtggc cgaaagatca tcgtctctgc tggtatcctg tcttatctgc
     5101 tttatggtgc tatctgtagg gccaactggc cctggtggta gttttttggc cctagcacaa
     5161 tttttggggg aaaatggcca tatttttgtt atcgggcgca gaggcattcg actcagtgtc
     5221 atggccgttc gccctagtgc ggtctcgtag tccgttctca tagacctgcg attctctgca
     5281 tccagagcgg tgtttgccga cattgacgat ccgcgtgcag ggctgtcaac gtttttgcat
     5341 atccctcgtt tcatttccat ctctcctcac acgcatcctg tcctgataca caagttttgt
     5401 cccttactgt cccctcctgt cccctagtgt ccccgaacag gctctttcac cactcacgcg
     5461 ccgttgcgta tggtccgctc atacgtcgtg cgctgaaccg aagaggagac gcgatgaaga
     5521 aaacatggtt aacgatcgat gaactcgccc tggaacttca agtcagcgtg aagtccatcc
     5581 gccgtgccta tcgaaagggt cagattcccg tggaacgaat ctgccggttt gtccgctttg
     5641 atctcgatcg agtgaaagcc gccatgcagg gaaacgagca aggacagtct cctttctcca
     5701 cagggagcca gggacagcgc agcgcgaccg gcggcggcag ccggcggcgc gcgcagcggc
     5761 cccgcccccg acttggtaag acgggggcgt ctatcgcaca ggccccaagg aggaagaaat
     5821 gacggtctcc ggcggattca cgcagaccat cgattggctc gccttcacgc ttccaaaagc
     5881 tgaggtggcg gacgtgatta cgctgattgg tggcgactgg ttccagagtg agaccggctt
     5941 tcgcggctat cccgtggctc agcttatgac agaaggcaaa acgggcgtcg ggaaactggg
     6001 gacgggtgct cctcgcaatc cgaaggaagt gcatgtcgat ctctcggcag gcatcgtctc
     6061 ccagtgggac gaaacgaagc tgaagacggt cctcgcctgg atcttcgctc aaaaaggcca
     6121 tgtgacccgc attgatgtgg ccctagatga ccgggaggcg acggtcgcag ttgaaaccgt
     6181 tcgccaagcc gtggaggccg gacaagtggt gagtcgatcc aaacagttca aggtcatcca
     6241 ggcttcgaat catcgccagg gagtccgaac cggagagacc ctctatttcg gcagtcggga
     6301 aagccaaagc atgctgcgag tctatgacaa gcggttggaa ctgaaaagtc gtggccggga
     6361 agacgcggaa tcctacggcg tccgctggga aatggaattc aaacaggatc gggcgcaagc
     6421 ctgcgccaaa gcgctgctta ccctcgatgc cgaagattgg cgggcctttt tggtcggagt
     6481 gctgcgttcc tatgtcgatt tccgagagac gacgcgagag gccgaatcgt acgagaagta
     6541 tcgcgcgccg ctcttggctt ggtgggaagg cttaaccgaa ggcttcaggc gctgtcggct
     6601 cgtggtcgaa cgcattcaac agcggctgga cgatgtcgtc gcgtggttcg ccaacgctct
     6661 cagccccatg ctcgccgtgg tcgtggcctg tcgcggtgat cagtttctga cggagatgat
     6721 ttatgcgggc acgaaacgat ggaaccagaa gcactatgcc ctgttgaagc aacgcaagaa
     6781 ggtggtgacg ccctatgtgc ttcatctcaa acctcggaca taacgtggtg tacgtccgtg
     6841 cctgtccaga tccggattgt aaggccgccg gagcctgggg tgagtttcgg aagggaaagt
     6901 tttttgtggt ctgtaaggcg tgtcgcttcg aatttcaatg tctgccgaaa gtccggcttg
     6961 cgggccgggg cgtgtgggtc aggcgcagag ctgatccgag ggtcttttca caatcaggag
     7021 ggtcgaacga tgcaagtcaa agcagagggg gcggtgcagg ggtatgtgga gcggaggagc
     7081 cgggagggca aggtttacag atcggtggat ttttatgtga agggcaaaga tccgggggtg
     7141 ctgcgattgg ggattccgga ggatcagatg ccgttgattg acgcctgcaa gcaggccgaa
     7201 gggaaacagg ctcgcgcgtc gatcgaagtg cggaagttcg agcagaccgg acgcacgttt
     7261 ttcgatctca ctggcttgga cgtgcagaag taacggcaga gggaggaagg ccggtggatc
     7321 tgacgattat tctcgtggcg gtcctgttac tggccttcct caccggcctc ggagtcggac
     7381 ggttgtgaaa ctcttcacgt taaccagtta tctctcgctc atctggggcc ttctggtgtc
     7441 gttctgtgta gcgccgggcg aatcccttgc gcaaaccgcc acgcagtatt cgagggtggt
     7501 cgcccaagct gaacgcattg cctatctggc tgcgcaacgg tcggctctgg cctctcaagt
     7561 cgccacggcg gccctggccc cctctgctac ctcgttggcc gttcgtatgg tcgcaggtcc
     7621 ggtcggttgg gccgccctcg gggtcagcgc gggcttggtc ttggcccaga tgtactattc
     7681 acagccggat cttgccgcca tcaaacaggc tgcgtcccca ccaacgggca actggaccta
     7741 ttcctacaat ggacagacct acatcattcc gaatcacaac atcgcgggca gtccgacgcc
     7801 cgcagcgaac tgtcctggcg ggctcatgtt cggctatctg ggcagtccca cgccgggcat
     7861 gacctccgcc gggcccacga actggtggtg tccgcctagt cctgcgcaac aaaccaccgg
     7921 cacacccacc gcatccgata ttcagcagta tctattgacg caatcgccca ccgatccgaa
     7981 tgccatcgaa caacatacaa gcccggtcgg taccaccggc acgacgcaac cggcagacaa
     8041 taccgtctcg cagccagtca gtccgactga aatgccgacg acggtcaagc ccaagccggt
     8101 ccctgccggg gatatcgtgg tggccgacaa cgtcccgcct ccggcgagca cgccgcagca
     8161 gaatactcag caacagacca ccaccacgac gacgacaacc acgcagaacc cggacggttc
     8221 gacgacgcaa gaagaagaga cccaggcgac gacatcctgc agcgttggat ctcatgagag
     8281 tcgaaccttc gggacggtcc tccagggaca tcaggcacgc tggggcacga gtggcctgct
     8341 tggcacgctg aatctcttga agtcgctgac ctggccctca accttgccgg tgattgcgct
     8401 gccttcggcc ttctttggga gtcagcaggt ggatttcaat caatgggcct ggttcttcac
     8461 cgtgttgagg actctcgtca ttgcgacggc ctctattgtc gcataccgca tcatcttcgt
     8521 cggtggaggc caaacggcat gactgcgatt ctcacgctca tctattgctg gctgcaggag
     8581 ttcttcttct cgctcacgga ttggggactt ggcatctggg attcgctgct ctccgtggcg
     8641 gacagcacgc tcgccgccat tgggacggca ggcctcaccc tgccggtgat tcctgatcaa
     8701 tatgcgtggg tgctgggcgc gacgggcatg agtcaggcgc tggccatcct ggcgagcgcg
     8761 atgggcaccc ggtttattct tcaaaccatt ccgttcgtcc ggtggggctc atgaaccaac
     8821 tcgtgatcgg gtgggcctat gcctggaccg tcatcttagc cgtttggatt tgggtgtatc
     8881 tgaggagtct ccgatgatcg aactgtacga aggggtgcca gggtcgggga agtcctatca
     8941 cgcgatctgc gagaagtttc tgccttgggt gaaacagggc cgacgactct atattgcggt
     9001 ggatggcatc tatctggacc gactttcatt gttcacgggt atcgaccttg aaacactcga
     9061 acaacagatc acgatttgga aagactcggt cgaagtcctc caggcctttc cccacgtcga
     9121 gccgggctct gcggtgatta tcgacgaagc acaaaccgtg ttccggtcca tgcaaaaggt
     9181 cgagccgggc ctcctgcgct ggctcgaaac ccatcgacac tatggcgtcg atattctgct
     9241 catgagccag gacttccggc agatgtccca aggtgtcacg cgactgattg aagcgacagt
     9301 gaagtttcgc aagttggcct tcgtgggttt gtcgaaaaag tatcagggca aggtgcgggg
     9361 taatccggaa gataacgaag tcattcgggc cttcgttggg agctactcgc cagcgatcta
     9421 cgcctactat tcgagctacg cctcggcggc catccgagaa gaaaaacgca gccatgcggt
     9481 cttcaagtcg gcacgggtcg ccatcggcat tgcggcgggc ctgtttgcca ttggccttat
     9541 attctggcgg ccttggtcgt cgttgagcag cacgaaagga actcccacaa gtgcagtgag
     9601 tggcacttcg agcttgcctc cgcttccatc tgcatcggcg ccgggcagtc tgctgaatcc
     9661 gttcggtgcg tctgtcccat cgacagcgag cccgcaattt cccaagcgaa cggtgcgcat
     9721 cctcggcggg gccggttcct cggcgaatcg tcaaggttgg cgctatctct tggacagcgg
     9781 cgagatcctg acagcggcac aaattaccgg acggtatggc ctgctggtct cggaagttta
     9841 tgaggatgga tcgatgcggc tcattggcga aggagtcttc tatggacctg ccggcgattg
     9901 aggcgatgac ctatttcacg gcgatcttct ggttgggtgg cttcgccgtg ggactgatta
     9961 tcaaactgat cctgcctcag actcactgag gcgctcgtcc gccggcgagt catccggtgg
    10021 cgtgggtgga cggaaccacc accagcacaa ggaggtgccg tatgaagggt tggagttggg
    10081 tgaaagggct tgggggggtg ctcatggcgt tcgtgttcgc cttgggtgtg ccggcgctgt
    10141 cgcaggcgca attgtttcca gtctcggcag atgtggccac ggtacgggcc gatctgctct
    10201 tgtgggccac ggccttgatt ggcgtggcct tggcgatcta cgcctttaag cgcgtgcgcg
    10261 cgattgtggg ctgagtgagc ccatcgaggc aggctgacag cagcctgcct ccttttccac
    10321 catgacgaag gagagggagc atgacggcgc aacaaattca aaccattggc aatgcactgg
    10381 ctgccgacct ggtcgcctgg ggcacggcgg cgattgggct cgccctcgta gccgctggcg
    10441 cagcctgggt gttgcggctt ctgcgctaag cgccaggcca gccctgatcg gcgaagctgt
    10501 agtagcgttc ttcggcgagg atgagatggt ccagcagcgg aatgccgaac agatcagccg
    10561 cctcgcgcag ccgtttggtc agggcgcggt cttcgaggct cggcgtgatg tcattggagg
    10621 ggtgattgtg ggcgcagatc caggcaccgg cattcatcag gatcaggggc ttgaagacct
    10681 cgcgaggatg gacgatggtc agattgagcg agccggtgga gacgacattg atcccgatca
    10741 gaccatgttt cgcatcgaga ccacagatga ggaactgttc ccggtccaac ccggcgaaca
    10801 aaggccggag gatcgccgca gcgccttccg acgtatgcac cgattccgca gccggaatgg
    10861 cacggccctc acggaccagc gtgacacgat accgggggat gccgtatttc cgaaccggtt
    10921 tcagtggccg tccaccgttc gaggaaccga gagagctatc aacggacatg tccgcctcct
    10981 tctgaaatta tgttctagcc ccacccttca ccaaccaagg gtggggcgta acgaaggagg
    11041 cggcacgggg ctctcgcagc gggcaggcct ggaggggcag agaccggcac ggaggggacc
    11101 gggaaggcct gtcccgctgc tccccagacc cgagtcgtag ccacaggggg cttactcttt
    11161 gactcaagcc gacggccatg tgggcgcttc agtgagggag taggggtgtc gagggactcc
    11221 ccggttaaat cagatcatcg ggactcaaat gcttctgctc aattgtgcgc gtagatggga
    11281 gaaagtatga cgcttcttac gatcgaagaa gctgctcggt tcttgcggct aacaaagcgg
    11341 acattgtacc aacgtgctga tattccccgt gttcgctatg gacatcgtct gatgttcgtg
    11401 aaggaagact tggagaagtg gattcgtatg cgctccgagg gacgggaggt gagtagcgac
    11461 aagcacgagg ttctgtctcc tgccccagtt gacgccggtt ccaccaacgt ctaccatcgg
    11521 aatccacttt ttgtattgtc tcgtcacagg taacgccatg aacattcggc gcatcctagc
    11581 ttctaggaag attcgttcgg ttctcaccta ttggtatcgc ggtgttcgtt ataggcctgt
    11641 ccttggatac aacctctcca ttgaccagga gcgtgaagct gctcttcaaa tcatcaccgc
    11701 tatacacgca aacacggcgc agccggtacg ttcagcctct cagccgattc agttcgctga
    11761 ccagtcggac accacctttt cggccttcat cccaatctac ctgcagtatt tgaaagcgaa
    11821 gcggccaaca aacgacggcc gaaatgaagt cattctgact aagcacatca ttccctattt
    11881 cggacagaaa agactcgctg agatccggct tgaagacgga ttggcctatt tggagaaacg
    11941 tcgctcggat gttacgggcc cacgtgataa ccgtcgacca gttgcggcag ggacgatcga
    12001 gcgagaatgt gccgtgttaa tggcggtgct caacctggcc gtggatatgg atcggctcga
    12061 taagaatcgg cttaaacggc ttccggtgcc ggagtatgtg aaacgtgagc gtgtgttgga
    12121 aggatgggaa ttgttgaaac ttaaggcggc tgcttctcca caagtatggc gtctcatcct
    12181 ggcagcgctc caaattggct tgcgcgagaa caagctcatt gaaactcatg aagaatggct
    12241 gatgcagcgt ggagacgggt ggtggatggc gccggcgcct ggtcgaacga agatcaaggg
    12301 tgttcccaaa acaatcccac ttaaccgatt ggcatatgaa gcactgttcg gagggctatc
    12361 acggattggc ggacgattct ttggccaatg gaaggatggg aactccttca agcatagttg
    12421 gatgagaacg tgtgagcgag ccgggattca tggcctgcac ttccacgatc tgcgacacac
    12481 ctttgcaact tggttgatgc agtgtggagt agattatgcg gtgattcaga ccctgaaagg
    12541 ggagccgctt cctggttcag cgaagtacta cattcacaat tgggatggtc gattgcgcga
    12601 tgcggtcact cggttggagt cgttcactga ggctcttctg aatggcgaaa atgcctctga
    12661 ggtgccactt actgccactc aggtgccacc atcctacgta gaacaatctc taagtccgcg
    12721 atttatggtg ccgagggaca gaatcgaact gtcgacacca gccttttcag gacatgctct
    12781 ttgtctgcaa cgaattgaaa ctatttctga tttgtcgtcg cggtgtacct ggaccaccag
    12841 atggacaacc gatcacttca ggttacattc tgaggatcca ccgcttactc caattcggga
    12901 tgttgggagg ggagtgcact ttgacacctt ctctagaggt gagcacaact tttacttgtt
    12961 cgatgtggcc gaaccctgct attggccaat gtctctcatt gtgggacagg ccgtgagaga
    13021 atgcccatgt tcgtccgctc agatcgtcaa gatacgactt gatcatgaac tcgttggcga
    13081 gtctaatggc actgtgcaga gcttatcccg gtctgacctc gagccaggtt catgaatagg
    13141 cagaaggata cacacatgat gatttgcttg ttccgaaacc agctcgggat cactctgtta
    13201 actctggtgt tgctcttctc cggctgcgcc tcgaagccgc cgcaggagct ttccgcagcc
    13261 tgtcccgacg ttgccaagac cggacaactc aacgttctga ccctgaatac attgtacgac
    13321 gcaccggccg ctgtacgaac gaagagctgg gccgacatcg cgcaattcgc cgtggcgaac
    13381 aatgttcatg tgttgctgct gcaggaagcc ctgttgaccg atgtcgatca gatccaacag
    13441 ctgctgggca cgtccgacag tgccagggac cttcagcgga tcctgaacgc gcgcagcccc
    13501 gagccgtacg agctgcgtgt agcctgggaa accggggttc ctctggtgct gaccaccgcc
    13561 aatgcgattc tgagtcgctg cgacgtcacg cggcattttt cgacgttctt gcccatcgaa
    13621 tccgaagagg ccttcgaagg aatcgaactg aagattacac gcaatgtcca agtggctcag
    13681 atgagtattc cgggttatgg gaatcttcac atctataaca cgcacctctg cgcggcctgt
    13741 tcgatcgaag ggctccagca gcaagtggct gcactcctgg cgttcgtgca acgggtcgaa
    13801 gcgaaggttc aaggcaatca tgtagtcctc ggcggagact tgaatctcga cctcgcgaaa
    13861 ggagtggcag accaggctgt gtatgagacc gtgacgcggg ccggttttcg ggatgtatac
    13921 gcagagtatc gaagaactca atttggtgag acgcgtcaga ctctgtgctg gaatggagtg
    13981 ccggatatcc actgcacgga cggggtgagc cccgtacaag gaatgatcga caagcaaacg
    14041 ggagcagggt tcccaaaacc gacaaggctt gattacttat tccttcgagg caccggcacc
    14101 gtgcagacaa gccgagtcat cttcaaccct ggcaatgcta cgaccggtcc catcaatcct
    14161 gccgagccgg cggtgtcgga tcatagcggt gtatttgctc ggattatgct gggcccctaa
    14221 taggcggatg aatcgagatc ctgacccgct cggcgaaact aaaccgattg ggatatgcgt
    14281 ggtcgaccac cagcaaggtg tcagagactc tgcgtcaggc ggcctcatgc tttatagact
    14341 ctcgaccatc ctcagttttt caggtcgatg agcattcaat gtgcgcaacg tataggtatc
    14401 tcgttagata cggcctgtat cattcttgat acttcgtgaa tggtaggata gcgacacctg
    14461 gcataccgca cctcgctgat tctcgccacg taagtctgta gagcgcatgc cacccacaca
    14521 gggcttctca ttgagagggg acagtatatc tactggttga agagtgggca gcgaaaacca
    14581 aaccgggtga agcatttcac cacaactgct ctcatatcgc tggccccaaa cgcccccacg
    14641 ccgacgtctt tccacacaca tatgaggtct ccagtgggca cagggttcgc tcgtagccct
    14701 caactcattg gggactcgta tccagtgaac cacatgcccg gccaggtatt ttccttgcag
    14761 gactgtcggg gtagccacgt ggagaacagg gcaagtccat cgcaaacttc acggaggtgt
    14821 cgtgcgtgtt gacgaatctc tcgatcaaga tatcaatcgg cctctttctt tcagcgctca
    14881 tctttgttct tcctacggtc cagccttccc aggcaggagt ggaggggggg acgcgtgggg
    14941 ttgaggtcgt cctgaaagag ccggaggtca tcctccggtc gctctacgat aagtcctggg
    15001 cgatcgtgat cggcatcaat cattatccag ggggagaccg caacacgccc aatctcaact
    15061 atgcagtgtc cgacgccaag cgggtggctc aaaagttggc ggggctgggc tttgaagtca
    15121 gaacgcttat cgatgaacag gcgacccgtc agaacatcat tcacctgctg gcggacgata
    15181 tcggccccca aaccaaggaa aacgaccgga tcgtgttcta tttcgccggg catggggcga
    15241 ccaaggagaa ggcggataag acctacatgg ggtatatcct cccccatgac tacaatccga
    15301 gcaaacatag tgcgacagcc atctccttgc agcaactgcg agaggcctcc tctgaaatca
    15361 aagccaaaca tatgttgtac ctgatggact cgtgcttctc aggagggctg ttggcaggcc
    15421 gtgggcggat gccgacggcg ggaacggcag gctatcaata cctgttgaac attaccaagg
    15481 gccgaagcca cgtggtgatt gccgctggag gcaaggatca gacggtgaag gaagaggggg
    15541 ggggcggggt gttcaccaga gtggttcttg acgcactgtc acaacgttcc aacatgccat
    15601 gggccaagga tggctatgtt accgccatgg atcttgcttc ctatgtcatg aagcgagttc
    15661 cggaccttgc gccgcagcaa acgccgcagt acggacattt ggaaggggaa ggcgatgtcg
    15721 tggtcgacat ttttcagcca ctcgatgtct cgggaggcga cgagcgggca cataagatct
    15781 tcgaagccga acaggccttg aaggctgagg aggcccttca acgggccaag cgcaaacgca
    15841 atatcgtggc gccgggtttc taagaccagt tcgtgatctg caccgttctc cgcaacgcac
    15901 ctattaggga gaccgaatca tgaaagggat cgtgttctct ttcgtggtga ttctcaccac
    15961 gttcactttc aacccggtat tcgcgaggga tcccggaggt cctgaggagc cggaggcatc
    16021 cgagaagccg ctcttctcac tgaagaccga cgaccgcgag ccgctcttcg gcgatcggtt
    16081 cacgctcaaa gccggctaca agatttgggt ggcgaaatgg caagcacagt tttccgcgac
    16141 gacaacagcc caaaaccagg tgaacacaga ccatcccatg gccttgaatg ggccgactgt
    16201 tactggcctt ctgaaactgc gagacagcag ttggttcaat gccgcatttt tcaattttgt
    16261 ctggctgcac gacggagccg acttcgctga acagcgtgat gattccagcc acatcttcgc
    16321 gaatagacga gactattcat tcactgctgg tttggccgtc tgggaagggt ttggcgtatt
    16381 cggcgggtat tacaacagcc gtcaagagtt cacaaaccaa ccaaacctgc cggggctcgg
    16441 cgtgtcgagg catcctcgcc tggtcgaggg tcccattgtc ggagtcttcg ggaatgcgca
    16501 ggcgtccgag catataggac tgtacggaaa tttagcgctt gcgatgctga gtttcagtgg
    16561 gttctgtccg tctcccaatt cctgtgcccg aactgactcc gcgcaaggct acatgaatga
    16621 ggtgggggtc acgattcagg gaccacgggt ctggaggatt ggaaccgagt tgcagctggg
    16681 cttccgcgcc caaattatcc aaaagaattt tggaatcaat gctaccggcg cggggacacc
    16741 gaatgcctca aatcagcagc ccttgctgga cgtgacgtac ggaccggtgt ttacgataca
    16801 tgcggtgttc tagctgaagt gtcgagatac caggagggct tccatgagag cgatacggat
    16861 gatggtggtg ttggttgcac tgagcagtct gctctccggc tgccagcggt tgtgccatgt
    16921 gctgtgcgat agtccgctca atcccgaggg ccagcttgcc gttttctcgg ggcaagtcct
    16981 ttccaccaac aatgtgcctc tgacggaagc gagcgttacc gtcaatggac gcgccatgac
    17041 aaccgacgat aagggcttct tcacgctgac ggtggattcc tcgtcccgct acctcgtgac
    17101 cattcggaag cgtggctata gtttgttctc acgcatatat accaaaggga ttctgaacaa
    17161 gaattggagg ttgacgcgag caacgctgac gaccttggat cctgccgccg gtggaattgt
    17221 ccaagacact cttgcagcca actgcctcgc gaaccgttcc cgctcaggct ggacccatct
    17281 tgaagtgaag ggaaaccctg tgtctccggc cctgagaact gcactggatg gtgcgtacca
    17341 accggccggt tgcagtgcag gcgtgtcggt ggacattccc gccgacgccc tggtggacgc
    17401 caatggcaat aggccgccgg ggcgcgtgga ggtggaggtg gcgacggttg acgtgttgtc
    17461 gccggaggcc atgccgggag actggggggc tcagttttcc atgcccagag aaggtggcga
    17521 gcgcctcgga ttcatgcaaa cattcggtgc cggttccgtc tcggtcacag caggagggaa
    17581 gtcttatcaa ctcaaaccga agaccacggc aagacttacc attcccgtcg atcccaccgc
    17641 ggctcagatt tacaggcgaa tggaacaacc cttgccgtcg agcataccgc tcctcctcta
    17701 cgacgaaaaa caaggcatct ggaaacagat gagtatcgct cagttgaacg agtcgagaga
    17761 tgcatacgtg gccgagctcc cccacttctc cgaatggaac atggacgtgg tgttttctga
    17821 tgtggcctgc acccagatcg acagccgtct gatcagcgga gggtattact tgaaagcagt
    17881 tccgtcagtt attcctgcgg gaggattgca gccgcacgag ttcgacattc ccaacacgtc
    17941 tggctgcttg ggaaacacaa caggagatcc gttctactgt tatatccatg cggcctggag
    18001 attgccgagc agtcctgccc atggttccgg ccaggtgtcg tttatgccgg gcaccaggac
    18061 tgggccgaat tcgggcgaca tcgtctataa tcgggcgaat tttgtagtcc cctttggtcc
    18121 cgccgcatcg aaccagcagc aaatgccacc gtcctacagt caacacccga cacaaccggc
    18181 cctccggcca ggaagttatc caaattgtac gacagccgtg accttcgctg ataagcctac
    18241 ccttcaagta acgtcaaccg cgacggcctt gaccttcacg cccagtggtt attgggggcc
    18301 caacagcacg atcgttccta accacccgga cgaccgctat gagtttcagt ggtgcaatat
    18361 cgcagcgaat ccttcctgct catccaaccc tgatgacagc ggatggatca atttcgcatc
    18421 ccttccgtcc atctccataa cgggcgcggc agtcaggccc gcgcgtgctg tcacgtcatc
    18481 gccgattcaa aaaagctccc ttccagccgg cacatataag tttcgagccc gggctcacat
    18541 gggggacagc tcgttcttga ttggcgggtc tgaaacgcca tggagcgatc catcgatcac
    18601 aatcaccatc ggaggcaact ccccacccac actcacctcg attgcgaatc agaccgtcaa
    18661 tgtaggtacg ttggtggcgc tgacggcaaa cggaaccgat ccagacggca atgcgttgac
    18721 gtacagcctg acgacggctc catcaggagc ggtcattaat gcgagtaccg gtgggtttac
    18781 ctggacgcct ttgggtgcgc aaggggccct gacccacaaa gtgacggtgc tggtgaccga
    18841 taacggaacc ccgaacctca gcgcgagtac gtcgttcacc gtcaccgtca atccacaggt
    18901 gctcattgaa aatcgtctgg gcggggggcc gggcactgga tgtaccagtc caccgatcga
    18961 caccagcgtc atccgtctcc aagtcgaagg tgttcccaaa ctaaccccgc tttctgatga
    19021 tgccgttgcg ggcgggaatg atccttcgta tcctaatgag ggaatataca tcactgccgg
    19081 gtcggattcg ctgacgttta acccgacggc cactacctat acagtcgtga ttgagctggg
    19141 ttcctggact ggtggcggga gcggggcggg gggacctttt gtccaacgcc ggtatactaa
    19201 ccatggtgct gctcccgaga catgctttgg ccttccatcg taccgatata tggatgttac
    19261 cgttaatcaa ggtcccggcc cgctcagatt acgaatcgag cctaccggca atcaatatca
    19321 ggtgatcgta cagagtggaa cggcaaccgt gaatactcca gcggccggcc agaaatcgat
    19381 cacagacttc acacgaacgg caggacccta caccaccccg tagaagcctt tgagctatgt
    19441 ctgactcttg ctgtagcatg caacgcggtc ttggcctata cacggaggca agcgcccagt
    19501 gtagccactc ggatcatccg accggtaagg cattaacccg atcggtcatt ggtgtttcaa
    19561 gaaggggaac caacatggca gattggatag tgaaactatc catgacaggc ttcttactgt
    19621 gggtgatgac tagcggcatt gccgcgggtg ctgaggttaa gattgaaaac cgcgtgggaa
    19681 gcggatcaac caatgggtgc aacacgcggc gcgacaccag catcatccgg ctacgagtcg
    19741 aaggtgttca caaattattg ccgcttcatg atgatgtcgg tgcaggcggc aacgatcctt
    19801 cctaccctgc agagggggtg tacatagctg ccgggtcgga ttcgacggtg tttaacagca
    19861 gtgccactac ctacagggta gtgattgagc tgggctcttg gaccggtggc gggagtggag
    19921 cgaatgggca attcacccaa gctcgtttta cccacgataa tgccaccatt ggtggttgtg
    19981 acaacccgcg ctatcgatat cttgatttta ccgtcactgg ccaaacccct ggcttactga
    20041 cattgcgact ccagcccatg ggcaatcagt atcaggcggt cgtccagggc gggatggcaa
    20101 cgctgagtac ggcggcgaca ggccagaaaa cgaccaccga ttttgaacgt acgactgagt
    20161 atatcccctt caatgaaaag ccggtgatga acgcgcagcg cttcagcgtg gcagagaata
    20221 gtaccgccgg caccgtggtg ggtaccctcg tagccacgga tccggatggg ggaccgctga
    20281 cctacagcgt cacgggtggg acggggcagg gggtctttgc ggtgaatccc acgagcgggc
    20341 agatcaccgt ggcaaacagc agccggctcg attttgagac cgagccgagc ttcgcgctgc
    20401 aggtgcaagt gagcgacaag gggacgccac agctaagcgc cagcgcgacg atcacgatcg
    20461 aattgactaa cgtcgatgaa ggagctcctg ccgcccctaa gaatttgagg atacgctgag
    20521 ccacaacctg agggctccca tcgctggtga cgaatacggg atggtcctct tggagtttaa
    20581 agtggtgagc cgttcggtat tattggttcc ctcggatacc ggagagaaat cggctctgct
    20641 ccaaagcgct cttactgaca gtggtcaggc caaaagcggc ttccgcccac tgggctggct
    20701 ggtcagggtt ggccacgacc ttgacgcccc gcatctgggc gtgaaagttc ttgagataac
    20761 tctggctttc ttcgacaggc ttctctgaag ctaagaggga atcgagtggg cgagtactag
    20821 ccaccgcctt gatggtttcg gcaccgaacg gaccagtgat gcggaacccc gagcgtgcat
    20881 gcctgccgcc gaaggtgtag gtcttgcccg cccggacaaa ggcgtcatcc acaaacatat
    20941 tgggaaccag gtgtacaacc gtgccatcag cctgatagta gtcaattttt aggaacgcat
    21001 cgcggttcga gcgaacggaa atttccaact cctcgccctc tgtataaact tcacctgccg
    21061 gtttgttcgt ccagagctgc aagtcgaagg aagagccttc cgtcagtacg ggaatctgtg
    21121 ccgcatcagc gatctgcttg gcgttcgctt tctgctgaat cacgtcatcg aatttgacgg
    21181 ggctgatctt ggcggtaatg gagatgcaga tttcctgtcc cttctgctcc tgtttgtcga
    21241 tgtggatttg ctcgagcatg cctgaactgg ccgtggtgac gatgtcttcc tccaaccgca
    21301 aattcttcac cgtggacgtg gattgcacaa aaacccgatg agttcggacc gcctgttctt
    21361 gggccagggc cagggcggcc cgcttggcga gggcaggagt ctgatagtcc ccataactgt
    21421 agcagccggt tccggaaacg gtctccagcg gtggagtcgc caagacagat tggcccggca
    21481 gggcccagcc catgactgtc gttgcgacag caagcaggat tcggctccat cgaggcgtca
    21541 tggcggggag ggctggtctt ctcacggcca tggattcatc gtcctttgtt tcgacacagt
    21601 tgcgcatgtg atgttccaga ggcgtaggaa gcagagcagc tctgagtccc gctgtgatag
    21661 agggcgccca ttccaggcat ggaataggat tgatgatctt cttgtcaagg gcaagaggcg
    21721 aaggggggca atgagagccc caatggcggg gcttcacaaa gaggaaagga aacgggggag
    21781 gggatcggag ggcagattcc gaacctcctg cgcgtcagtt cgatggacct gctttcttaa
    21841 aggggttggt ggtgcttggt ggcagatccg gggaacgctc atggggcatg acgatgtggg
    21901 cacggttctc ttgcgcattc tgcgcggctc attgattttc tcgaaggagt acattgttga
    21961 gaaacccgcc gagttcggga ttggcgtagg ttccaaaggc atccgcgatg atccctctgg
    22021 taccatcgtg actctcgatg gcatagacga cggacatgtc ctccggatcg ctggctcctt
    22081 cgaaccgata gtgttccacg atgctcagtt cgtcagccgt aaagattttc tcgctgtcga
    22141 cgtcccggaa ctcctgatcg agaaattcga aattcgcagt aaatccccgc tttcttaatt
    22201 cctgaatggc ttcggtcata ttcgtatacg atgcgacact catcgtgtac ctcccgctct
    22261 cggacatcct tctcctcgga gaatctcagc ggggcttatc tgcctccatg cagggagacc
    22321 agtctaagac cgttctcccc gcatcgggcc ctgcgctgcg tcaaggagtc gcctgacagg
    22381 atacgagctg aactcttacc ggccccaatg gatgtcgacc atgacgttat ccgcgtcgag
    22441 ttcaatggtg acaggcgtgc cttcctgcaa cgtggagagt ttgctcccgg ccagtggatc
    22501 tacgtcaaaa ctggctggcc cttcgggggt cgaaagctta atggcgctcc aaaggggatc
    22561 cgtatagtga agccggccgg caacaaagtg atgctcagga aagaactggt ctgttcgtga
    22621 gacatccatc agcacgttgc ccgaatcgac gagcaggttc acggtttctc cgatcttggc
    22681 gtcgtacaga ccgacacgct ccgccttggt gggactgatc acacgcgcct gcaagcttgt
    22741 gtcggtcttg acaaagagca tgccggattc gatcttggtg atcaccccgc tgaaaccgta
    22801 atgcgcgctc tgcacaaagg gcgttgcctg ctcagcgccg gctggggcaa caaccagcgc
    22861 catcattgct atcgtcagaa agagggaaag tgtaggtttc atagggtcac cctcctttca
    22921 cgcagccatt ggagaacaat cactgaatca ctggaaccat agacagcttg atgccccctg
    22981 accatcgccc gaaggattga ttttactcgt gaggggccaa ttccctcgat gctacagtcc
    23041 cccttgctgc acggcagcca agcccggatg agccatgtgt gcatcacgat cagccgccaa
    23101 tgagactcct tgcaccgagc cggatgagtc gagtgcctga atctacccat gttcggcaca
    23161 gactcccgac gaccttgcga ttctcgtgcg tgcacgacct aaaaatggag tgaagagtaa
    23221 aagttccatt cttttctacg aggttcaatt cagatcagtt gtcccttcct gcgtcagagc
    23281 ctgactccac agctgtagca tcttgtcctt gatatcgccg tcctgcttgc tgaaaaatta
    23341 ctgcaacctt ctgtttatat ttcattttca gcgactctcg accttgctca ggggcggcac
    23401 agggattgat agataaagtt ccgagaagcc catgtgggag ttcgtatcgg acagggtggc
    23461 aatcggatat cggaagcaaa tgtcagtggc ttgaacggta agaaatactc gactgacggc
    23521 gaacttgtcg ccagaagctg gaggcagcta tgagtgcagg ctgtggttta ccagagataa
    23581 gcccgtttat cggctgggaa gttcttgaga tcaaaggaaa caagggcgtc atgctgaatg
    23641 gctcatctgc gagaaagaag atccccatag ttccaaacca tagttcctgg cggaccctgt
    23701 ccctgagctt cactcttagc ctgcttcccc tgctgtgggt gccggtcagc gaggggcaga
    23761 ccacctcgat tacgtcgtct gggttgggca cgacgatctc acccgctagc atcgaccctg
    23821 ccggtaggcc aaattacatc attacaggcg ggactaggcc aggtgacgga cccaatctgt
    23881 tccatagctt tggggacttt agtgtcggga cgaacaacgt ggctcgattt tttaacgagg
    23941 ccggccgctc taccacgaac attctcagcc gggtcactgg agggcagacc tcaaatattt
    24001 atggaacgat tcagacggcg gggtttggaa ctgcggcgtt gtggctgatt aacccggccg
    24061 gcattgtctt cggcccctcg gcttcgctga atgtcggggg ttcggtgcat ttcagcacgg
    24121 cggactatct gcggctcggc agcggcaacg atcgattcta cgcggattta ggcaagacga
    24181 gccaactaac cagcgcacca gtgacagcgt ttggattttt gggtgagcgc ccggcaggtc
    24241 ctgtcaccgt gcaagccgga tcgccgtttg ccgttggtga aggaaagacg atttctctga
    24301 taggcgggga tatttcggtc accggacgga cgctgtccgc cccaggtggg cagatcaact
    24361 tagccagtgt ggcaggaggt ggcgaagttg ttctgaatca ggtaggtcag acgccttctc
    24421 tcgacctcgt gaacgtttcc aatcaaggca cgattcaact cacagaagga gcgattttaa
    24481 gaacgagttc tgccacgagt gatgcaggtg ccatctttat ccggagcggt caatttttca
    24541 tggagaatgc ggggcttgag gcaatttcga ccgcaccatt tgacgctttc aaccccacac
    24601 caacgggtgg ggcggtagac atcctttcag aagtcatgag cataaagaac ggtgcactgg
    24661 acctgcctgg ggctggaagc ttgcctcagg gacaactggg atttgcagga catattccag
    24721 gagtgagcgt tctaaccaac aatagcggcg acattacgct gggtacgtca actctcagtc
    24781 tgaataatgc gaagattctg agttatggtc agcacattga ttcgcaattt attgcgccgg
    24841 accgttttac ggggtacatc catattcaag ggactggggg agcaggatcg tttgcggatc
    24901 gcgtggccat tgaaaatagt agtcttcttc acaccgcagg ctttgccagt gactgcaatt
    24961 gtactgtatt tgctcgagac gtgcgcattc agtccaacac tgtagacatg gcgaatagta
    25021 ggatatcgac aaatgagggt atcatcgcca tctcagcctc gaatcaaata cttagccacg
    25081 gcaataatac gatcgccaag gagttcgggc aacgggacct tcgaggaggc gagaatgcta
    25141 ttgagcttac agcgggagat gtgattgttc tcggcaaggg cgatacaatt tctactggcg
    25201 cacagggctc gagttttaca ggggggggta gcagggctgg cgacattgtc ctgaatgctc
    25261 ccagcattac aatgaatggc acaactctta aagccgacgg taacttcact gttaacggcg
    25321 gttcgataat cctccgctca ggcgcaatag aactatccga atcacagctc agcacgaacg
    25381 gtcttgacgg ggggattcaa cccttttctg gaattgcagg tgacattctc ttgagcggcc
    25441 gccaagccgg tgtacaaacc aacaccattc acctgacgaa tagttcgttg gaaagcgcag
    25501 tcctaaaaag ttcccaatcc tctaccgcct tcggcgacag cggtaatatc acgttagaag
    25561 gtcaacatgt aacgttggat cactcgtcgg tttctgtcgc tcatgaagga gtcgggaaga
    25621 gtggctttat aatgttaagt ggagggcagg tcgattttgt caataattca gaactcataa
    25681 gtgccgcgag tgggaagagt atcccgtttt cttctgagtt cagcagatct gcggctgccc
    25741 caggtggcga cattgcgatt caagcgatgg acattagttt agcccaggga tccaggacaa
    25801 aagcgtcttc ttccggtgat ggcaacgccg gcgctattaa tctcatctcg attgacagta
    25861 tctccattgc aaacagtacg gtatcgactt ctgccactca agcgtcaggt ggaaacattg
    25921 atctcacggc tccgaatctg gtgcgactcg ttagcgcgaa tgtcacgagc tcagtgaacg
    25981 gtccagccaa ctccaatggt ggcaacatta ccatcgacac ggcgcaccct caatttgtca
    26041 tcatgcaagg caactcccag atactagcca aggctaatga agggcaggga ggtgccatca
    26101 ccattatcgg cggagtggtg cttcaagagc cagggagcgt gctcgacgcg acagcagggc
    26161 ctgctggaat cagcggctcg attaacattc aagcgccatt ccaacagctc gccggtgcca
    26221 ttgcgccctt accgcaagcc tttgcggtgg cagccagcct ctacggtcag cgctgtgcga
    26281 ctgagaaagg cgggcaattc agcagtttcg tgcaaggagc gcgagacgga gtccctccgc
    26341 aaccgggaga tctcattccg agcccgctca tgctggagct tgatgaggcc gcgttcagca
    26401 ggggctcgca gtcaatccca agtcttgccg caatccgtct cgggcttcct gagttcgagc
    26461 agacgtctca cagcctgaca gtctttgccg gctgtcgatc ctgagctgtt gatcccttcc
    26521 tgtcttcgca ctgatggtcg gacgcaagga ctttcgcagg caggctgaat gtggagaata
    26581 cgtgcatttc acgaaggtga tgcctgtgta acaccggcaa agtgatgaga aaaggttcca
    26641 ttcttttctg ctgagttcga ttcggatcat atgtcccttc ctgcctcgga gcttgacccc
    26701 acatctgtag cgtcttgccc ttgctgtcgt cgttctgctc tctgaagcat tgttataacc
    26761 gtctatttac gcgtcacttg gagggttggc tgaacctcgc tcgttaggca caggtattga
    26821 tgtataaaga ataggagggg cccgtatgtg gggggtgcgt atcggagaag gtggcgcagg
    26881 gtggcaattc ggtatcggaa actagtggac tgtaacaatt gattcgggag taatgcgacg
    26941 tgacgcatcg aagtac
//
LOCUS       DAORRQ010000056        24426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_119_length_24426_cov_49.195889, whole genome shotgun sequence.
ACCESSION   DAORRQ010000056 DAORRQ010000000
VERSION     DAORRQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119_length_24426_cov_49.195889"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PK224_15570"
     CDS             <1..1308
                     /locus_tag="PK224_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17214.1"
                     /translation="RYLTNYPKSRRALEAHWDLGQAYEQMGEVTAAIKEYRTLAGPEG
                     APLSAQNSYAERAQHRIDALRNQPASPAGARSGHTALYVSFSNLPPMSQVEAWIRQLS
                     AQGISAILMDASLESSFTRPLIPTSPPTSSPLPTLPGGALFATSRGPVLTDWYGVMVP
                     QAHELGISVYAVLDLYHAPLSDHRPESKMLLYDPTRRKVHPWTQFDLLAPAVQQQMAQ
                     LLADLLKTGVDGLVFRSRAENSFAYEVSDGVLRQFETQVHQPSSEVAQALRDRMALRP
                     NEPAPNSDKTLWRWVGWKARQELDTLARFKKYLQKAVPRFRMVIEIHPEALSNPTSAL
                     VNYGEDAAEARRRGFDLLLGGASREASDVRSFGGAFKGWSRDVIQSEKGEPQTLPQGW
                     VLLRTPAEHGGGMFTGLAQQADELRTPDIRHLVFVPDAPVVIP"
     gene            1404..1790
                     /gene="gcvH"
                     /locus_tag="PK224_15575"
     CDS             1404..1790
                     /gene="gcvH"
                     /locus_tag="PK224_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRB17215.1"
                     /translation="MIPSNLRYHQEHEWVRAEGAQATVGISHFAQDALGDIVFIDLPK
                     VGAKVTAGQQIGEVESTKTTSTIYTPVSGTVAKINGDLKDHPEVVNSDPYGKGWMAVI
                     DLTAPAEVDQLMTAAQYETFLSTQKH"
     gene            1798..3219
                     /gene="lpdA"
                     /locus_tag="PK224_15580"
     CDS             1798..3219
                     /gene="lpdA"
                     /locus_tag="PK224_15580"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRB17216.1"
                     /translation="MNHLAILGAGPGGYVAAIRAAQLGARVTVIENQALGGVCLNWGC
                     IPSKALLSVVELGDKAKKAKDFGIQLSGPVTYDPAVMVARKNKVVSTLVKGIATLFKT
                     WNIEHLEGTGELLDARTIRVTKPDGTETRVVADGVIIATGSSWPNLPLFPIDGTQIIT
                     SKQALDLSRIPVSLLIVGGGVEGCEFASLYSGLGTQVTLVELVPRLLPLEDEEISQMM
                     ERELKKRGVDIRTGVTVDQIVRQPDLVTAHLRDGLSLNVEQVLVSVGRGFNSRGIGLE
                     KAGVQVGTRGEIVVNERMETNVPGVYAIGDVVGKAMLAHVASAQGKVAVENFMGRPRT
                     IDYDVIPTGIFTLPEIGRVGLTEQQARDRCVAAGKDPQQSVKVGRFRYGGLGKAQATG
                     DIQGLLKVVADAESDRILGVHILGAHAADLVHEAALAMHLGATVSRVAEMIHAHPTLA
                     EGLMEAMEDVHGHAIHLARKPSS"
     gene            3219..3761
                     /locus_tag="PK224_15585"
     CDS             3219..3761
                     /locus_tag="PK224_15585"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRB17217.1"
                     /translation="MLRSLLIKVAMLAVTLGSLIWIGSVTPVRQIPSHPVRPAAEVPA
                     PATVRQVSIAPPQAVSSADGSSLVAKVPDVTVAPTVEESETPVRQEQPARGLRPVHRE
                     AARQVDLNHATASDLQGLPGIGPKLSQRVIDHRTARGPFGKIEDLQQVKGIGRKKFDR
                     LRPHVLVTNTRSLARHKGTL"
     gene            3758..4975
                     /gene="tyrS"
                     /locus_tag="PK224_15590"
     CDS             3758..4975
                     /gene="tyrS"
                     /locus_tag="PK224_15590"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRB17218.1"
                     /translation="MTSVAQQLDLILRGIVEVINPNELESKLARAIKEQRPLRVKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHQVIFLIGDFTGMIGDPTGRSETRVALSKEKVL
                     ENAKTYERQIFKILDPQKTQVEFNSRWMSAMTADGLIELSAHYTVARMLEREDFHKRY
                     TEGKPISIHEFMYPLIQGYDSVALKADVELGGTDQKFNLLMGRDLQRDYGQEAQVVIT
                     MPLLEGTDGVRKMSKSLGNYIALEDKPGEMFGKVMSISDALMHRYYELLTTEDLERVK
                     TAHPMDAKQSLAELIVARYHGAEAGREARAMFQQKFQSREFPEQPDARVILTPSDVKD
                     AADPMIGIVDLVMKTGLVPSKSEARRLIVQGGVEINEKKLMNVNASVALSAGKPLYMR
                     VGRRKFAVAEYTV"
     gene            5045..5119
                     /locus_tag="PK224_15595"
     tRNA            5045..5119
                     /locus_tag="PK224_15595"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5077..5079,aa:Gly,seq:ccc)
     gene            6269..7735
                     /locus_tag="PK224_15600"
     CDS             6269..7735
                     /locus_tag="PK224_15600"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRB17219.1"
                     /translation="MTDFNKQNVAEGAPVLSPSPLESESSADLHTLPLESLKAVLDIT
                     ALALEPERFTGSTSAVVTALASRLACDRVSLGIATRNQLRVQALSHSAQFNHKTDLLN
                     AITASMEEAVDQQQTVLVPAPAGWPAQSTRAHDELRRRYGAASVCSLPLWAHGRIVGV
                     LTCERGGEQPFDRFTVELCEAMAALVGPTLDLKRRHDRALPFKIWESVRRQIGTVIGT
                     GHMGVKLSVLLIAALGSWFLTSAGEFRVTSKAVLEGEVQRVAAAPFQGYIRTAPARAG
                     DVVKAGQVLATLQDRDLQLERLKRLNEREQLAKEQRQALAERNAPKAEILSAQLRQVQ
                     AQLDLATDKLSRTQIVAPFAGVVVSGDLSQQLDAPVEEGKVLFEVAPLDTYRIVLQVD
                     ERDIGHVAVGQSGELRLSALPQELLPFEVAKITPVSTAQEGRNFFRVEGRFLTKPSQL
                     RPAMEGVGKITIDRRLLAWIWTHDAMEWVRLKLWAWLP"
     gene            7748..9895
                     /locus_tag="PK224_15605"
     CDS             7748..9895
                     /locus_tag="PK224_15605"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17220.1"
                     /translation="MAESFFSPSWYRVEGLQPRLRSHLRVHRHQYRGQTWYVLQDLAT
                     DQFHRFSPEAYGLIGLMDGRRTVQEIWEQASERLGDEAPTQNDVIGLISQLHTSDALF
                     FEAPPDMVELSKRSATQVRRKRYTRLANIFSWRMPLCDPDRFLARFLPLVRPLISLPG
                     LLLWTALVGMGLLWGGTYWTALFHDFSDQALAPQNLVLLWLLFPLLKLCHELGHGFMA
                     KAFGAEVHELGVMLLVFTPVPYVETTGAWGFRSKWQRILVSAAGMMVELALASLALIV
                     WINAEPGLVRVLAYNTILIAGISTVLFNANPLLRFDGYYMLMDYLEIPNMKHRAGRYF
                     SYLAERYLLGQPEAEMPPATAGERVWFVLYGTASSLYRILVVVGILLFLGDQFPPVAI
                     LFAVFTGVMMVVVPVVKGATYLFTSPRLHLVRTRAVVTVVCLVAALVGVVGFVPVPFR
                     TVTEGIVWLSDDAMVRAETEGFVTQVVATPGSQVQAGDVLFICRNADLRAQLNVLNSR
                     LQELKARHTEQEPNDRTKAAIIEEETKYVTQERDRIRERVERLVVRSKQSGTFVTPRA
                     EDFPGKYVRQGDLMGQVLDLRTVTVRTVVPQGEIDLVRYQLESVDVRLAERLPATQPA
                     ALVRLVPAATKQLPSAALGSQGGGQVPLEPSDEKGLTAMGRFFQADLTLANSMGLMDA
                     GGRAYVRFNHGWMPLSAQWTRQLRQLFLARFNV"
     gene            9888..11879
                     /locus_tag="PK224_15610"
     CDS             9888..11879
                     /locus_tag="PK224_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRB17221.1"
                     /translation="MSRSLELSVIQGLAYPERSAPRAGKLDRWAQAAVAPVLRRLRAS
                     VVRRERWVQQVAAAGVECARLREEGVKSMAQDLRASLTKAGFRPALAAKAFALVREVA
                     SRTIRMRHFDVQLVGGWILLTGRVAEMETGEGKTLTATLPACTAALAGLPVHIVTVND
                     YLAARDADLMRPVYESLGLSVGTIVQGMTPSQRQAAYACDITYCTGKELVFDYLKDRI
                     LVGTASNRLQVQLDRLHRRDAQSGGFLLRGLQYAIVDEADSVLIDEAKTPLIIARCTD
                     NTAEQTTYAQALHMARQLLEGRDFVVDRSERTVRMMPCGEARAKELAVGLGGLWRGRR
                     RREALVHQALIALHLFHRDQQYLVQESKVQIIDEYTGRLMPDRSWEHGLHQMIQTKEA
                     CPLDNQQNTLARISYQRFFRRYLRLAGMTGTAHEVARELWSVYRLGVVRLPSNRPLRR
                     RRITDQVYVGADDKWQAVVRSVAEMTQRGRPVLVGTRTVAASEHLSRLLESAGLEHQV
                     LNARQDRDEAAVISRAAEPGRITVATNMAGRGTDIRLDPDVVARGGLHVIATERHDSG
                     RIDRQLFGRCGRQGDPGTYQTIISLDDQLFVDHGGSVGRRLRGMLGCLSTRIPRWLGN
                     FFCRRAQQRAERLQARMRRAVMKMDEQLDSTLAFTGRSE"
     gene            11889..12764
                     /locus_tag="PK224_15615"
     CDS             11889..12764
                     /locus_tag="PK224_15615"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17222.1"
                     /translation="MRLTGCGKIRLLRNTLRSTDAPCRYHNNPKDVQKGRPARPQQAK
                     RRIVLAPYGELLSAARTPLADFFRILLRTFASYSVSAASIGSLLLLSAPGVLQAGELS
                     CLIQPYVVITITSPVGGLLETVAVDRGDLVKEGQILAVLDTSLERATGAVAHAQAELT
                     NRRLADLELQRTSAEVGLRTIRSPINGVVVERYMSPGEFPKQERIMRLAQINPLRVEA
                     YVPVSLLGKITVGMEMQVKPEAPVSGTYKAVVTVVDRVVDAASGTFGVRLELPNPDLK
                     LAAGLKCAMVVPGSK"
     gene            12827..14899
                     /locus_tag="PK224_15620"
     CDS             12827..14899
                     /locus_tag="PK224_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRB17223.1"
                     /translation="MRDGGYFSKLREFLSNERRGRTRSLSKRRRVKRASPFRIEQLEA
                     RVLLAGDLASVTLQAAVVQEQYVEGAVNLQNGTQDSGYRYRVRQDFGTQADSSAAPTV
                     SKLRIYENGVELGPAHSRHDDIATKGQGRFSHWVDNLYFSASDNSNPLTNGRSYTFRV
                     YTDGSTSATTTPTTTVPTTTTSPTTTTSTYVQGAVNIQQASQDSGYRYRVRQDFGTQA
                     DSSAAPTVSKLRIYENGVELGPAHSRHDDIATKGQGRFSHWVDNLYFSASDNSNPLTN
                     GRSYTFRVYTDGSTSTPTPPPSTTPTTPPTTPTTGGSVKTDFGVYKEPALPSLPSSGS
                     KIVDPTFGTTILRLTDGRDGSTDAMVGYSNLPSFNKDNTYVMAVEQLGQKRAKFYTFD
                     PVSFSSGSGFVLSKPPSGLQEYGLIWSGVNRQVTFGVGQNKIWQVDVSTQQSTLVKDL
                     TSYGAGGYLTQMSKSLNDDVFGASVVNGSGSTVGYIVYKRSTDTVLLRNTVSNLDEVQ
                     VDKSGRYFIAVYKDGHDEIWDLQAGPKLTATLSGSAGFKHRDTGFGSAFTSYSGNSLG
                     FRQLSSPTTVKALVPGYWGYSANNQQDHFSLLADNEGWGLASRYSTTGSGVAKTFDNE
                     IVLVATNGSNQVRRLAHHRSVVNDYYDQPKANISRDGQFIAFTSNWGNASGRRDVYIV
                     KVPPAPMI"
     gene            14983..15828
                     /locus_tag="PK224_15625"
     CDS             14983..15828
                     /locus_tag="PK224_15625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17224.1"
                     /translation="MIFIGNKRATSRLAVAMVRFLCVVLLGFSAGLVEAKSELSCLVV
                     PQVTLTIGTPVVGVLNSVAVNQGDVVKEGDILATLESSLEQAEVALAKAKAEMDAAEK
                     STQMKAEFSLRKSVRAKDLGRTSAMAHHEVDEAETDERLAQVAHIEALENKRVAQLEL
                     ERAKAALELRTVRSPITGMVVERFLSPGELVKQAPIMKLARLDPLIVEVLVPVSWFGK
                     ITAGMEGDVRLEWSSPDLHRARVTVVNPVVNSASGTFEVLLELPNPGYKIPAGLSCTV
                     RFAVH"
     gene            15972..17309
                     /locus_tag="PK224_15630"
     CDS             15972..17309
                     /locus_tag="PK224_15630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB17225.1"
                     /translation="MKMYEKRRDVMAMIFAILLIGGTSAQAQPGGAVPDSLPELRLSL
                     REAMEAAVDNNPNVRLLKERIETARAASKTQLGALLPNLSTNVRQSRQNIFLGTIGLA
                     PVRTDPFSIFDARANVSQSLFSLSLIQRWRASREALKVAELESETTKFDTMSAVGLLY
                     VETLKAEATVKTRNANMQLFEELLELARSRRGGGMGTGLDTARLEAQLENERQQLAVA
                     RAEVERLKVNILHGLGFAFDVRLTLTDALKLDVLEPPTIEEAVTAAMNQRMEVKAQQQ
                     RIKTASLTLNSTETERVPSLAAQGDYGLIGNRMHNTLDTYNIGLFLTVPLFDGAREGR
                     ISESRSQVNQETIRMRVVSNQVVLDVREALVTLSSAKEQLAISQVGLQAALKELALAK
                     ERFTVLTAGSNFEVTNALYSLSRARDNTVDAMFRLNGARVNLARALGELDKLS"
     gene            complement(17631..18338)
                     /locus_tag="PK224_15635"
     CDS             complement(17631..18338)
                     /locus_tag="PK224_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17226.1"
                     /translation="MSRIVEVQTTTVVAIVTNNYLLRLGLQKIVDDEKWIRLVGQAAH
                     AGNLDEILDNEQPHIVILDTEIGGTAPDLIQKIKRVAPGVKTILLSGFDDTECTRQAL
                     ASGVDGIVLKMQPSAVLIATINYLADSGREVVLPIGHSPAPLKLSHIPAIPTTPPPPP
                     APTKWSDGLTEREREVVRLISEGLSNKDIADRLCISSITVRHHLTSIFDKLGVSNRQK
                     LLIRAHQQGLVGSPALA"
     gene            18871..19434
                     /locus_tag="PK224_15640"
     CDS             18871..19434
                     /locus_tag="PK224_15640"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HRB17227.1"
                     /translation="MLKTIQAGSRNAHSLTTDAAVGLQWYAISTRSRHEKLVRDRLAG
                     IGIEPFLPLVKKLSQWTDRKVWTESPLFSGYCFARFSLMNSHAVLQTPGTVRIVGSLM
                     PEAIPDEEVAAIQKLAESRRAFERHDYLTEGSWVEVVRGPLTGLRGQLLRKANQDCLV
                     IRVHLIQQAATVHIDMSEVQPVVQSLA"
     gene            20086..21360
                     /locus_tag="PK224_15645"
     CDS             20086..21360
                     /locus_tag="PK224_15645"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB17228.1"
                     /translation="MGWAWFSQFANPALVGALYMTPDWPKTGYYPVYPGAFSRPITED
                     GIAVGPARLHPILGVAEIYTDNVFRTNSNRRSDFATTLAPGIQAELPFAGRHTFVADY
                     RSNIQLFHQNPSNNVQDQTASGRFNFDFPGGLRLDVQGEHKLGHDPRGSALDTQNVEV
                     NKWTANSVLGRLSYDGATAGIRLNLSSTRWTYLNNEQSIFRNRLSNYAGVTVLGNVTS
                     KTALVANVAANQEVYDDSKTLNSTTYSASTGLQWNVSEMTTGEILAGYQHLKFANAES
                     GQSVAGLQFPRSSDSYGNFFFVSNVSWKPTPFLTLYLQGYRSFQQSVVLNTLFFTSTG
                     ANFSAVHTLTNSTALTLNVGVERDKFENPAPTPDNPNRVDLIKNVAFGVRYRTVKWAG
                     VALQYIFEDRSSNVDRFNYYANSVMLSLQATF"
     gene            21526..23721
                     /locus_tag="PK224_15650"
     CDS             21526..23721
                     /locus_tag="PK224_15650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRB17229.1"
                     /translation="MNAKAVVLDEAEQTLSEYAVSCWRHRWLIVLVAGIFGFGAAGWS
                     FLQVPVYQAKATVVIENPTPGGLDKDKSFYPDNSPEYFQTHFELMKSHFVLQRTARLL
                     ELSDRPEYQPKPSAIRQFLKSLLPDSVLTFLRPAQKEKADSVDDAEEALIKQFAQDIE
                     IMPIRGARLAHVTANAIDPELAAQIANTLVSVYIERNQELSTNSKEQAAQWFTGHLEE
                     LRQKVQNSQQALYLFRAKHGLLTGEERKSVAAHTLAELNSQLVKTEMAKAEAHSRFLQ
                     IRSVLNPQGAGAAGVDWSKLDSQIQVLNSPLIQTLRAQEITVSAQVAELSEKYGPLHP
                     KLAHMKAELQDLRQRIQQEVQKIYDSVKHQYDMALAQDKAVKEAITRYSTDKIRLEQN
                     EIEHSMLERDAETSQHLYDVFLKATKETDLSSGLRANNVYLADPAAPSSVPSKPRTKL
                     NIMLGLLAGLMSGVGLAIFVDGRSKKLMAPADVERYLPNVSLLGVVPLLSKAAAQEKA
                     LAPPNALTPIAESFRIIRTSLLLSNPDMLPSRILITSPGENEGKTTLAVNLAMAMAQL
                     EDTQVVLIDVDFRNQHIHPLYDVGSPEQPPKGLGHLLRGEAVLAEVVHPTFLSNLSVI
                     PHGGRPANPTELLHSKSMAQLLNWGATNHYHIILDCPPVLPIADAAVLASKVDGVLLV
                     VSAGETTRDACHLAIQRIKQAGGRLLGVVMQKAPVADSSYYYGAYQQQR"
     gene            23780..>24426
                     /locus_tag="PK224_15655"
     CDS             23780..>24426
                     /locus_tag="PK224_15655"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRB17230.1"
                     /translation="MNHSLRIGLKRWGTWQRQASNRRVLAALFTVGGYSLLGKVSAFA
                     KDAVVAYQFGRGDELDAFLIALVIPQFTITLLGGSLNAALIPTYIRVREQEGPEAAQR
                     VFSTVTLLTSGFLLLTCFILMLSAPWLMPILAAGYGPEKLVLAKVLYGVLLSTVLFSG
                     IGTTWGAVLNAGNRFAWAAAVPMVTSLTTMVAVLCLARSWSVYALAAAAVTGAFV"
BASE COUNT         5018 a         6597 c         7583 g         5228 t
ORIGIN      
        1 cgatatctca cgaactatcc gaaatcccgt cgcgcgctgg aggcccattg ggatctgggg
       61 caggcgtatg aacaaatggg tgaagtgacg gcggcgatca aagagtaccg gacgttggcc
      121 ggtccggagg gcgcgcccct ttccgcgcaa aattcctacg ccgaacgcgc ccagcatcga
      181 atcgatgctc tacgcaacca accggcttcg ccggccggcg cgagatccgg tcataccgcg
      241 ttgtatgtct ccttcagcaa tctgccgccg atgtcccagg ttgaagcctg gatcaggcag
      301 ctgagcgcgc agggcatcag tgccatcctg atggatgcca gtctggagtc gtcctttacc
      361 aggccgctga tccccacgag cccacccacg agcagcccgc tgccgactct gcccggtgga
      421 gcgttgtttg ccacgtcgcg tgggcccgtg ctcacggact ggtatggggt tatggtgccg
      481 caggcccatg agctggggat ttccgtctat gccgtgctcg atctgtacca tgcgccgctg
      541 tccgaccatc gaccggagtc caagatgctg ctctacgatc cgacgcggcg caaggtgcat
      601 ccctggacgc agttcgatct cctggctccg gccgttcagc aacaaatggc gcagctgctg
      661 gcggatctgc tgaagaccgg tgtcgatggc ctggtgttcc gcagccgcgc cgagaatagt
      721 tttgcctatg aggtgagcga cggcgtgttg cgccagttcg aaacgcaggt ccatcagcct
      781 tcctccgagg tggcccaggc ccttcgtgat cgcatggcac ttcgtccgaa cgagccggct
      841 ccgaattccg acaagaccct ctggcgctgg gtcgggtgga aggcgcgtca ggaactcgat
      901 acgctggccc gcttcaagaa atacttgcag aaagccgtcc cgcgcttccg gatggtgatc
      961 gaaattcatc cggaggcctt gtcgaatccg acgtccgccc tggtgaacta cggggaagat
     1021 gccgcggagg cgcgtcgtcg tgggtttgat ttgctgttag gcggggcgtc ccgcgaggct
     1081 tcggacgtcc gatcgtttgg cggtgcgttc aagggatgga gtcgcgatgt gattcagtcg
     1141 gagaagggcg agccgcaaac ccttccgcag ggatgggtgt tgctccgcac cccggcggag
     1201 cacgggggtg gcatgttcac ggggctcgcg cagcaggcgg atgaactccg cacgcccgac
     1261 attcgtcacc ttgtgtttgt tccggacgcg cccgtggtga ttccttgaca aagtttttcg
     1321 cagcttttac gatcgcgcaa cccggttccc gtgcgctttt attcacgtag gccgctcgtg
     1381 cagcggccgt cacgaggaga gtcatgatac cgtccaatct tcgttaccac caggaacatg
     1441 aatgggtccg tgctgaagga gcgcaggcga cagtcggcat cagtcatttt gcccaggatg
     1501 cgctgggcga cattgtgttt atcgaccttc cgaaggtagg cgccaaagtc accgccggcc
     1561 aacagatcgg cgaagtggaa tcgaccaaaa ccacgtccac gatctatacc ccggtcagcg
     1621 gaaccgttgc gaagatcaat ggcgatctca aagatcatcc tgaggtggtc aattccgatc
     1681 cgtacggcaa gggatggatg gcggtcatcg acctcacagc gccggctgaa gtggatcagt
     1741 tgatgacggc cgcccagtac gaaacgtttc tgagcacaca gaaacattag gcgcgtcgtg
     1801 aaccacctgg ccattcttgg tgccggtccc ggcgggtatg tggcggcgat tcgcgcggct
     1861 caactcggcg cgcgtgtcac cgtcatcgaa aatcaggctc tgggcggagt ctgcctgaat
     1921 tggggctgta tcccgagcaa ggcgttgctg tcggtcgtgg agctcggcga taaggcgaag
     1981 aaggccaagg acttcggtat ccagctgagc gggcctgtca cctacgatcc cgccgtcatg
     2041 gtggccagaa aaaataaagt tgtctccacg ctggtgaaag gcatcgccac gttattcaaa
     2101 acgtggaaca tcgaacacct ggaaggcacg ggtgagttac tggatgcgcg tacgatccgc
     2161 gtgacgaaac cggatggcac cgaaacccgg gtcgtggccg acggcgtgat cattgcgacc
     2221 ggctcgtcct ggcccaatct cccgttgttt cccatcgacg ggactcaaat catcaccagc
     2281 aagcaggcgt tggacctcag caggattccc gtgagcctgt tgatcgtcgg cgggggcgtg
     2341 gagggttgtg agtttgcctc cctgtacagc gggctgggta cgcaggtgac gctggtcgaa
     2401 ctcgtgccgc gcctcttgcc gctggaggat gaagagatca gtcagatgat ggagcgcgaa
     2461 ctgaagaagc gcggcgtgga catccggacc ggtgtcacgg tcgaccagat cgttcgtcag
     2521 ccggatctcg tcaccgccca tctgcgcgat ggcctgtcgc tcaatgtaga gcaggtgctg
     2581 gtgtcggtag ggcggggatt caattcgcgt ggcattggat tggagaaagc cggagtacag
     2641 gtcgggacgc gcggcgaaat cgtggtcaat gagcggatgg agacgaatgt gcccggcgtc
     2701 tacgcgatcg gcgatgtggt cggcaaggcg atgctggcgc atgtcgcgtc ggcgcaggga
     2761 aaggtggccg tcgagaattt catgggccgt ccccggacga tcgactacga tgtcatcccg
     2821 acgggcatct ttacgttacc tgaaatcggg cgggtggggc tcacggagca acaggcccgt
     2881 gaccggtgcg tggcagccgg gaaggatccg caacagtcgg tgaaggtcgg gcgcttccgg
     2941 tatggcgggt tgggaaaagc gcaggccacc ggggacattc aaggattgct gaaggtggtg
     3001 gccgatgcgg agagcgaccg tattctgggt gtacacattc tcggggcgca cgcagccgat
     3061 ctcgttcatg aagccgcgct ggccatgcat cttggcgcca cagtctcgcg agtggcggag
     3121 atgattcacg cgcacccgac cctggctgag ggcctcatgg aggccatgga ggacgtccat
     3181 ggccacgcaa tccatcttgc acggaaacca tcgtcctaat gctgcgctca cttctcatca
     3241 aggttgcgat gttggcggtg accttgggat ccttgatctg gatcggttca gtcacgccgg
     3301 tcaggcagat accgtcgcat cctgttcggc ctgcggctga ggttccggct ccagcaaccg
     3361 tacgacaggt ttcgattgcg cccccgcagg ctgtgtcttc ggccgatggg tcgagccttg
     3421 ttgccaaggt ccccgatgtc acggtcgctc caacagtcga ggaatccgaa acccctgtgc
     3481 gacaggagca gccggcgcgc ggcctgcgtc cggttcatcg cgaggcggcg cggcaagtcg
     3541 atctcaacca cgccaccgca tcggatttgc agggcctgcc cggtatcggc cccaagttgt
     3601 cgcagcgggt gatcgatcac cggacggcac gtggaccatt tggaaaaatc gaagaccttc
     3661 agcaggtgaa aggcatcggt cgaaagaagt tcgaccggct tcgtccccac gtgctcgtca
     3721 ccaatacccg atcactggct cgacacaaag gaactctgtg acttccgtag cgcaacagct
     3781 ggaccttata cttcgaggga ttgttgaggt tattaatccg aacgagctgg aatcaaagct
     3841 ggctcgcgcg atcaaggagc agcgaccgct gcgggtcaag gcgggcttcg atcccacggc
     3901 tccggacctg catctcggtc acaccgtcct gatccacaag ttgaaacact ttcaagacct
     3961 cgggcatcag gtcatctttc tgatcggtga cttcaccggt atgattggcg atccgacggg
     4021 gcggtcggag acgcgtgttg cgctgtcgaa ggaaaaggtg ttggagaacg ccaagacgta
     4081 cgagcgccag atcttcaaga ttctggaccc tcaaaaaaca caggtggagt tcaacagtcg
     4141 ttggatgagt gcgatgacgg ccgatgggtt gatcgaattg agcgcgcact acaccgtggc
     4201 tcgcatgctg gagcgggaag actttcataa gcggtacacg gaggggaagc cgatcagcat
     4261 ccacgagttc atgtatcctt tgatccaggg gtatgattcc gtcgcgctca aggccgacgt
     4321 cgaattgggc ggtaccgatc aaaaattcaa ccttctgatg ggacgggacc tgcagcgtga
     4381 ctatgggcaa gaagcgcagg tcgtcatcac catgccgctc ttggagggaa ccgacggcgt
     4441 tcgtaagatg agcaagagcc tcggcaatta tattgcgctg gaggacaagc ctggcgagat
     4501 gttcggcaag gtcatgtcga tcagcgatgc gctgatgcac cgatactacg aactgctgac
     4561 gacggaagat ttggaacgcg tgaagacggc tcaccccatg gatgccaaac agtcgttggc
     4621 agagctgatc gtagcccgtt accacggggc tgaagccggt cgcgaagcca gggcaatgtt
     4681 tcagcagaag tttcagtcgc gggaatttcc ggagcagccg gacgcccgtg tgatcctcac
     4741 gccctccgat gtgaaagatg ccgcggatcc aatgatcggt atcgtcgatc tggttatgaa
     4801 gaccggccta gtgccgagta aaagcgaagc gcggcggtta atagtgcagg gtggcgtcga
     4861 aattaatgaa aagaaactaa tgaatgtgaa cgcgagtgtt gctctgtcgg ccggtaagcc
     4921 gctttatatg cgtgtcggcc gccgtaagtt tgcggtggct gagtatacgg tgtaagtggc
     4981 cctcgccggt tgccttgact tagcagcggc tccggcgtag gattcgtttc ctcttccgca
     5041 aggggcgggc gtagctcagt ggtagagtcc ttgcttccca agcaagttgt cgtgggttca
     5101 aatcccatcg cccgctccaa aaaaccaatc cacattcagc agatttgaat ccacgacaga
     5161 gggtttcgaa gggagtatgc tcccttcgtg gggagacacg cgggggtacg cgacgcgagg
     5221 aataacgagc gtcaggtctg cggacgccgg cgagatagtg agccggccgg aacaggcccc
     5281 ctcagtggga gcagggaggc aagtttcagg gccgacccgg cgacggagtg tggttcaaat
     5341 cccatcgccc gctccaaaca aatctttccc gaccacgagc ctctttcatt attgttggcg
     5401 gaggagtaaa cccttcagtc gtcctttcgt gcgtcctcgt gcctacgggt ggcttttcga
     5461 acgcagcctt tctcttgttg ccgccggaga aacaccttcg gtggtcttcg atcctcttgt
     5521 ttgttccggc cgtaagatct tcatcactgc cccgggcgac cggatagtgc tccactgatt
     5581 ctcctttgac gatcctatgt ctactcgacc caaacgttgc atcaggtcga gcagcaatgg
     5641 tgagttggtg ccgggatggc cgcgggacca tggttcggga gcgtgcggaa ttggcgagtg
     5701 tcgaacggca gggatttcgc gaagcgtgtg tggcagtatg cggacggcga gcatcatctt
     5761 caaaacgtgt gcaagtaaag tctctcttcg ccgctttcga aagccgctcg gcgatcaagc
     5821 gacgagcgcg gttctcacaa catcctgcga gcgcccgctt ttggtgtgcg agtattcggc
     5881 ggtgccgggt tcgtcacgtc tcgattcacg ccgcccccaa caagagcctc ctgtacacgt
     5941 ttcgcgcact tcgctcgttt ccgctcagtg cccgtggccg ttgatcggcg gactgggcca
     6001 tgcgcggatg gtcgcgccgc actacggtgc attggggtcg gtcaattcgt agaggttgaa
     6061 tacgtaggat atgtggtgac tgggagtcgt acctaggtaa gccttcgctc ctaggccatt
     6121 tttacatctt gcaactattt gtcaagatag ttatgctgca cgtggcagtt gcgcttcggc
     6181 agctcatctc gccacttctt tcacggccca cgcggcactc aacaacaacc attcacgagg
     6241 gagctattaa ggcccacgcg tatacgtaat gactgacttt aataagcaga acgtcgcgga
     6301 gggggctcct gtattgagtc cgtcgcccct ggagagcgag tcctccgccg acctacatac
     6361 gttgcccctt gaatcactca aggccgtgct tgacattacg gccctggccc tggagcctga
     6421 acgattcacc ggatctacct cggcagtggt gactgccttg gccagccgat tagcgtgcga
     6481 tcgagtcagt ctcggaatcg cgactcgtaa ccaacttcgt gtgcaagcgc tgtcgcacag
     6541 cgctcagttc aaccacaaga cggatcttct gaatgcgatc actgcttcca tggaggaagc
     6601 cgtcgatcag caacagacgg tccttgtgcc cgccccagcc ggatggccgg cgcagagcac
     6661 gcgtgctcat gacgagcttc gccggcgcta cggcgcggcg tccgtctgtt cacttcccct
     6721 ttgggcccac ggcaggattg tcggcgtctt gacctgcgag cgcggtggcg aacaaccctt
     6781 cgaccgcttc actgtagagt tgtgtgaagc gatggcggca ttggtcggtc ccaccctcga
     6841 cttaaagcgc cggcatgatc gtgcgttgcc gttcaagatc tgggagagcg tccgacggca
     6901 aatcggtacg gtgatcggca ctggacatat gggagtgaaa ctctccgtgc tgctgatcgc
     6961 ggccctgggc tcatggtttc tcacaagtgc gggtgaattt cgcgtgacgt cgaaagccgt
     7021 gttggagggc gaggtgcagc gggtggctgc agcgcccttc cagggttata tacgcaccgc
     7081 gccggcccgg gcgggtgatg tcgtgaaagc cggacaggtg ttggccacgt tgcaggatcg
     7141 ggacctgcag ttggagcgtc tgaagcgact gaatgagcgc gagcaactgg ccaaggagca
     7201 gcggcaggca ctcgcagagc gaaatgcgcc gaaagccgaa atcctcagcg cccaattgcg
     7261 gcaggtgcag gcacagctcg acctcgccac ggataagctc tcgcggaccc agatcgtcgc
     7321 gcctttcgcg ggcgtggtgg tctccggcga tttgagtcag caattggatg cgccggtgga
     7381 agagggcaag gtgctttttg aggtggctcc gctggatacc tatcgcatcg tgctgcaggt
     7441 ggatgagcgg gacatcgggc atgtcgcagt cggtcagtcc ggggagcttc ggttatcggc
     7501 actgccgcag gagttactcc cgttcgaggt cgcgaagatc acgccggtat cgaccgcgca
     7561 ggagggaaga aatttctttc gtgtcgaagg ccgattcctg acgaagcctt cgcaactcag
     7621 gcccgccatg gaaggcgtcg gcaagatcac catcgaccgt cggctcttgg cctggatctg
     7681 gacacacgac gcgatggaat gggtgcggtt gaaattgtgg gcgtggctgc cctgacaagg
     7741 agatgcggtg gcggaatcgt ttttcagccc atcgtggtat cgggtcgaag gcttgcaacc
     7801 gagacttcgc agccacctgc gcgtgcatcg gcatcagtat cgcggacaga cctggtacgt
     7861 tctgcaggat ctcgcgaccg atcagttcca ccgcttttcg cccgaagcct acggtctcat
     7921 cggtttgatg gacggacgcc gcacggtcca ggaaatctgg gagcaggcga gcgaacggtt
     7981 gggcgacgag gcgccgaccc agaacgatgt catcggcctg atcagtcaac tccatacgag
     8041 cgatgccctg tttttcgagg caccgcctga catggtcgag ttatcgaagc ggtcggccac
     8101 gcaggtacgc cgaaaacgat acacccggtt ggcgaacata ttctcctggc gaatgcccct
     8161 gtgtgacccg gaccggtttc tggcgcgatt ccttcccctc gtgcgtcctc ttatcagtct
     8221 gcctgggctg ttgttgtgga cggcactcgt cgggatggga ctgttgtggg gcgggactta
     8281 ctggacggct ctgttccacg atttctcgga tcaggcgctg gcgccgcaga atctggtgtt
     8341 gctgtggctt ctgtttccgc tgttaaagct gtgccatgag ttggggcacg gcttcatggc
     8401 gaaggcgttc ggtgcggaag tgcatgaact cggggtgatg ctgctggtct ttacccccgt
     8461 accctatgtt gaaaccaccg gggcgtgggg attccggagc aagtggcaac gcattcttgt
     8521 gagcgcggcc ggcatgatgg tcgaactggc cctggcttcg ctggcgctga tcgtgtggat
     8581 caatgcggag cccggcctcg tgcgggtgct ggcgtataac acgatcctga ttgccggcat
     8641 ctccaccgtg ttgttcaacg ccaatccctt gctgcggttt gatggctact acatgctcat
     8701 ggactatttg gaaattccga atatgaagca tcgggcaggc cggtattttt cctacctggc
     8761 cgagcgttat ctcctggggc agccagaggc ggaaatgccg cctgcgaccg ccggtgaacg
     8821 ggtctggttc gtgctgtacg ggaccgcctc gtccctctat cgcatcctgg tggtcgtcgg
     8881 gattctcctg ttcttgggcg atcagtttcc gcctgtggcc attctcttcg cagtatttac
     8941 cggagtcatg atggtggtgg tgccggtggt caagggcgcg acctatctgt tcacgagtcc
     9001 gcgactgcat cttgttcgta cacgggccgt ggtcacggta gtctgtctgg tggcggcgtt
     9061 ggtgggagtg gtcgggtttg tacccgtgcc gttccgtacc gtgacggagg gcatcgtctg
     9121 gctgtccgac gatgcgatgg tccgggcgga aaccgaggga ttcgtgacgc aagtggtggc
     9181 gacaccgggt tcccaggtgc aggccggaga cgtgttgttc atctgccgga acgccgacct
     9241 gcgtgcgcag ctgaacgtat taaactcccg gcttcaagaa ctcaaggccc gtcacacgga
     9301 gcaggaaccg aacgatagga cgaaggcggc catcattgaa gaagagacga agtacgtgac
     9361 ccaagaacgt gaccgcatcc gtgaacgcgt ggaacgcctg gtcgtacgga gtaaacaatc
     9421 cgggaccttc gtcacgccgc gtgctgagga tttccccggc aaatatgtcc gccaaggtga
     9481 cctcatggga caggtgttgg atctacgcac cgtcaccgtc aggaccgtgg tgccacaggg
     9541 agagatcgat ctggtccggt accagctcga atcagtcgat gtgcgtttgg ccgaacggtt
     9601 gccggcgacc caaccggcgg ctcttgtccg cctcgtgccg gcggcgacga agcaactgcc
     9661 tagtgcggcg ctgggaagtc agggcggcgg acaagttccg ctggagccgt cggacgagaa
     9721 ggggctcacg gcaatgggcc ggttttttca ggctgatctc accctggcca actccatggg
     9781 actgatggat gccggcggtc gagcctatgt gcggttcaat cacggatgga tgcctctgtc
     9841 tgcgcagtgg acccggcagc tccggcaact ctttttggcg cggttcaatg tctagatcat
     9901 tggaattgtc ggtcatacag ggactggcct atcccgagcg ctcggcacct cgggcaggaa
     9961 agttggatcg ttgggcgcaa gccgccgtgg ctccggtcct gcgccgtctt cgtgcatcgg
    10021 tcgtccggcg ggagcgctgg gtgcagcagg ttgcggcagc cggcgtcgaa tgtgcccggt
    10081 tgcgcgagga gggcgtgaag tccatggcgc aggacctgcg cgcctccttg accaaggcgg
    10141 gatttcgccc cgcgctggcc gcgaaagcgt tcgcattagt gcgcgaagtg gcctcgcgaa
    10201 cgatcaggat gcgccatttc gatgtccaac tcgtcggagg atggatcttg ttgaccggtc
    10261 gggtggcgga gatggaaacc ggcgagggca agacgttgac cgcgacgctc ccggcctgca
    10321 ccgcggcact cgccggtctg ccggttcata tcgtcaccgt caacgactat ctggcggctc
    10381 gcgatgccga cctgatgaga cccgtctacg aatcattggg actttcagtg gggacgattg
    10441 tgcaggggat gacaccgtcg caacgacagg ccgcgtacgc ctgcgacatc acctactgta
    10501 ccggcaaaga gttggtgttc gattatttga aagatcggat tctcgtcggg acagcgtcga
    10561 atcgactcca ggttcagctg gaccgtctgc accggcgtga tgcgcaatcg ggcggctttt
    10621 tgttgcgggg cctgcagtac gcgatcgtcg atgaggccga cagcgtgctg atcgacgaag
    10681 ccaagactcc gttgatcatt gcgcgttgca ccgacaatac cgccgaacag acgacctatg
    10741 cgcaggcgtt gcacatggct cggcagctgt tggagggacg tgattttgtc gtcgaccgca
    10801 gtgaacgcac cgtgcgaatg atgccgtgcg gcgaggcgcg tgcgaaggaa cttgccgtcg
    10861 gcctgggcgg attatggcgc gggcgtcgtc gtcgtgaggc cctcgtgcac caggccttga
    10921 tcgcactcca tctgtttcat cgtgaccagc agtatctggt acaggagtct aaggtgcaga
    10981 tcatcgatga gtacaccggc cgtctgatgc ctgatcggtc ttgggaacac ggtctccacc
    11041 agatgattca gaccaaagaa gcatgcccgc tggataatca gcagaatact ctggcccgaa
    11101 tcagctatca gcgattcttt cgccgctatt tgcgtctggc cgggatgacc ggaaccgcgc
    11161 atgaagtggc gcgtgaactc tggtctgtct atcgtctcgg ggtggttcgc cttccatcga
    11221 atcgtcctct gaggcggcga aggattactg atcaggtcta tgtcggggcc gacgataagt
    11281 ggcaggcggt cgtccgatcc gtcgcggaga tgacgcagcg gggcagaccg gtgctcgtcg
    11341 gcacgcggac ggtcgcggcc tcagagcatc tcagccggtt gctggagagc gcaggactcg
    11401 agcatcaggt cctcaatgcc cggcaggatc gggacgaggc cgccgtcatc agtcgggccg
    11461 ccgagccggg gcgtattacc gtggcgacca atatggcagg gcgaggaacc gacatccgtc
    11521 tcgatccgga tgtggttgcg cggggcggac tccatgtcat cgcgacggaa cgccatgatt
    11581 ccggtcgtat cgatcgccaa ttgttcggcc gttgtggacg tcagggggat ccgggaacct
    11641 atcagacgat tatctccctg gacgatcaat tgttcgtcga ccatggtggg tcggtcgggc
    11701 ggaggctcag ggggatgctc ggttgtctca gcacgcgcat tccccggtgg ctcgggaact
    11761 tcttctgtcg gcgagctcag cagcgtgcgg agcggctgca ggctcggatg cggcgagcgg
    11821 tcatgaaaat ggatgagcag ttggattcta cactcgcatt caccggtcgt tcggaatagg
    11881 aacacgccat gcgtctaaca ggctgcggaa aaattcgatt gttgcgcaac acgctacgat
    11941 caactgatgc gccatgtcgg tatcacaata acccgaagga tgtgcaaaaa ggccgtccag
    12001 caaggccgca gcaagcgaag aggcgaatcg tactcgcgcc gtacggtgag cttctgagcg
    12061 ccgcgagaac gccgctggcg gactttttcc gcatcctgct aagaacattc gcgtcataca
    12121 gtgtatctgc cgcctcgatc ggcagccttc ttctgctgtc ggcccccggc gttttgcagg
    12181 cgggagagct gagctgtctg attcagccgt acgtcgtcat taccatcacc agtccggtcg
    12241 gggggttgct ggaaacggtg gccgtggatc gcggggatct ggtgaaggaa gggcaaattc
    12301 tcgcagtcct cgatacgagt ttagagcggg caaccggtgc cgtcgcccat gctcaggccg
    12361 agttgacgaa tcgccggctg gcggatcttg aattgcaacg gacttcggct gaagtcgggc
    12421 tacggacgat ccgtagcccg atcaacgggg tcgtagtcga acgctacatg tcgccggggg
    12481 aattcccgaa gcaggagcgc atcatgcgac tggctcagat taacccgctt cgggtcgagg
    12541 cctatgtgcc cgtgtcctta cttggaaaga ttaccgttgg catggaaatg caagtgaagc
    12601 cggaagcgcc ggtcagcggg acgtataagg ctgtcgtgac ggttgtcgat cgtgtcgtgg
    12661 atgccgccag cgggacattt ggtgtccgcc tggaattgcc caatccggac ttgaagctgg
    12721 cggctgggct gaagtgtgcg atggttgtgc cgggtagcaa gtagtgccgc gtattgttgt
    12781 caatgcggaa gaaccacgtt ttcttaacta ggaggggagg tcagtgatga gggatggcgg
    12841 atatttctcg aagttgcggg aatttttatc gaacgagaga agaggacgca cgcggtccct
    12901 ctccaaacgg cggcgtgtga agcgcgcctc tcccttcagg atagagcaac ttgaagcgcg
    12961 ggttctgttg gccggtgacc tggcaagtgt gacactgcag gcggcggtgg tgcaggaaca
    13021 atatgtggag ggcgccgtca accttcaaaa cggtacccag gattcgggat atcgctaccg
    13081 ggtacggcag gatttcggga cccaggccga ctccagtgcg gcgccgacgg tctccaagtt
    13141 gcggatctat gaaaacgggg tggaattggg gccggcccat tcgcgacatg acgatatcgc
    13201 gacaaaaggc caggggcgtt tcagccattg ggtagacaac ctctactttt cggcctcgga
    13261 caattccaat ccgttgacga acgggcggtc gtataccttt cgcgtatata cggacgggag
    13321 tacttcagcg acgacgaccc ctacaacgac ggtcccgacg accaccacat ctcctaccac
    13381 aacgacttca acgtatgtgc aaggagccgt caatattcag caggcgtccc aggattcggg
    13441 atatcgctac cgggtacggc aggatttcgg gacccaggcc gactccagtg cggcgccgac
    13501 ggtctccaag ttgcggatct atgaaaacgg ggtggaattg gggccggccc attcgcgaca
    13561 tgacgatatc gcgacaaaag gccaggggcg tttcagccat tgggtagaca acctctactt
    13621 ttcggcctcg gacaattcca atccgttgac gaacgggcgg tcgtatacct ttcgcgtata
    13681 tacggacggg agcacgtcga cgcccacgcc tcccccctcg actacgccca ccacaccgcc
    13741 cacaaccccc accacaggcg gcagcgtcaa aaccgatttc ggagtgtata aggaaccggc
    13801 gttgccttct ttgccttctt ctggttcaaa aatcgttgat ccgacgttcg gcacgacgat
    13861 tctccgtcta accgacggac gggacggaag caccgatgct atggtgggct acagcaatct
    13921 gccttccttt aataaggaca atacctatgt catggcagtg gagcagctgg gccagaagcg
    13981 cgccaagttc tacacttttg acccggtcag cttttcatcg ggtagcggat tcgttttgag
    14041 caagcccccg tcagggcttc aggagtatgg cttgatatgg agcggcgtca atcgtcaagt
    14101 gacttttggt gtcgggcaaa ataagatctg gcaagttgat gtgtcgaccc aacagtcgac
    14161 gctggtgaag gacctgactt cttatggagc cggaggctac ctcacccaaa tgtcgaagag
    14221 cctgaacgac gatgtattcg gtgcttctgt tgtgaacggc agcggatcta cggtcgggta
    14281 tatcgtgtac aagcggagta ccgacacggt actgcttcgg aacacggtga gtaatctgga
    14341 cgaagtgcag gtcgacaaat ccgggcgcta tttcattgcg gtgtataaag atggccacga
    14401 tgaaatttgg gatttacagg ctggtccgaa attgacggcc accttatcgg gcagcgccgg
    14461 gttcaagcat cgggatacag ggttcggaag cgcttttaca tcctattccg gaaattcact
    14521 gggattccgg caattgtcgt cgccaacgac ggtcaaagcg ttggtgcctg gctattgggg
    14581 gtatagcgcg aataaccagc aggaccactt ctcgctgctg gccgataacg aagggtgggg
    14641 actcgccagc cgctattcca cgaccgggag tggagttgcc aaaacgtttg acaatgaaat
    14701 cgtgctggtg gccaccaacg gcagcaatca ggtgagacgg ctcgcgcatc accgaagtgt
    14761 ggtaaacgat tattacgatc agcccaaggc gaatatcagt cgcgacgggc agtttatcgc
    14821 ttttaccagc aactggggta atgccagtgg acgacgtgat gtgtatatcg tcaaagttcc
    14881 gccggctccg atgatctaac gggcaggggc ggcaagcggc cggagtgccg gcttgccgct
    14941 cctcaagcgg atccaaggca cgaatacggc aacaaagaac agatgatctt tattggaaac
    15001 aagcgcgcga ctagtcgatt ggccgtggcc atggttcgat tcctctgcgt cgtactactc
    15061 gggttttctg caggattggt tgaagctaag tccgagctat cgtgcctcgt ggtgccgcag
    15121 gtcacgctca ccattggtac accggtcgtc ggtgtactaa atagcgtggc ggtgaaccag
    15181 ggtgatgtgg tcaaggaagg cgatatcctt gccacgcttg agtcaagttt ggagcaggcc
    15241 gaagtcgcct tggcaaaggc gaaggccgag atggatgcgg cagagaaaag cacccaaatg
    15301 aaggccgagt tcagtctgag gaaatctgtg cgcgcaaaag atttgggccg gacatccgcc
    15361 atggcacacc acgaagtgga tgaagccgaa acggatgaac gattggcgca ggtcgcccat
    15421 atcgaagcgt tggaaaacaa gcgagtcgcg caacttgagt tagaacgtgc caaggcggcg
    15481 ctggagttgc ggaccgttcg tagtcccatt accggcatgg tggtggagcg gtttctttct
    15541 cccggggagt tggtcaagca ggcgccgatt atgaagctgg cccggctcga tccgttgatc
    15601 gtggaggtgc tggtccccgt atcttggttt ggaaaaatta ctgccgggat ggagggggac
    15661 gtgcgattgg agtggtcctc accggacctg catcgggctc gcgtgacggt ggtcaatccg
    15721 gtcgtcaaca gtgccagcgg tacattcgaa gtgctgcttg aacttccaaa tccgggctat
    15781 aagataccgg ctgggctttc ctgcacagtc cggttcgccg tgcattagtt ccccgcctta
    15841 tcagtcactt ttccgcttcc tcaagcaaga caacaccaac ggcggcggag taggctgtcc
    15901 gaatgaggct tatacctgta ctcgtatgtc agggctccgt gtttcatgag gcaaggagaa
    15961 aggtggatcg tatgaagatg tatgagaagc ggcgagacgt gatggcgatg attttcgcga
    16021 ttctgttgat cggtggaact tctgcacagg cacaaccggg gggggcggtg ccggattcct
    16081 tgccggaatt gcgcctgagt cttcgggagg cgatggaggc tgccgtcgac aacaatccga
    16141 atgtgcgatt gctcaaggag cggatcgaaa ccgcgcgcgc ggcgagcaag acacaattgg
    16201 gcgcgctctt gcctaatctc tcgaccaacg tgcgccagtc gaggcagaac atatttctcg
    16261 ggacgattgg tttagcgccg gttcgcacag atcccttcag tatttttgat gctcgggcga
    16321 atgtgtctca gtcgctgttc agtctgagtt tgatccagcg atggcgcgcc tcccgggaag
    16381 ccctcaaggt ggctgagctg gagtcggaaa cgaccaaatt tgacaccatg tccgcagtcg
    16441 gcctgctgta tgtcgagact ctcaaggccg aagcgaccgt gaagaccaga aatgccaata
    16501 tgcagctctt tgaagagcta ctggagctgg cgcggagcag acgcgggggc ggcatgggga
    16561 cgggacttga taccgccaga ctcgaggcgc agttggagaa tgagcggcaa caactggctg
    16621 tggcacgggc cgaagtagaa cggctcaagg tgaacattct gcatgggttg gggtttgcat
    16681 tcgatgtccg attgacactc actgatgcac tgaagctaga cgtgttggag ccaccgacca
    16741 ttgaagaagc cgtgaccgcc gcaatgaatc agcgtatgga ggtcaaggcg caacagcaac
    16801 gtatcaagac ggcctccttg accttgaatt caacggaaac cgaacgcgtc ccttccctcg
    16861 cggcccaagg ggactacggt ctcatcggga atcggatgca caatacgctg gatacctata
    16921 acatcgggtt gttcctcaca gttcccctct tcgacggcgc acgggaaggc cgaatcagcg
    16981 agtcgcgcag tcaagtcaac caggaaacca ttcgcatgcg ggtcgtcagt aaccaggtcg
    17041 tgttggacgt ccgggaggcc cttgtgacct tgtcgtctgc gaaggaacag ttggcgattt
    17101 ctcaggtcgg tctccaggct gcactcaaag agttggccct tgcaaaagag cggtttaccg
    17161 tccttaccgc tggcagcaat ttcgaagtga caaacgcttt gtatagcctc tcccgagcaa
    17221 gggacaatac ggtagatgca atgtttcggc tcaatggggc ccgtgtgaac ctcgcgcggg
    17281 cattaggaga acttgataaa ctcagctgac cttgcgggga cagtgtccat ccagcgacac
    17341 agtcctttga ttcaatgtcg gtccgtcgtt ctgagcatat cgtctgtgac accactgttg
    17401 tgggaaggtc agcaccgctc ctacaagggc ggcagcacaa aaattgaagc ctgggaaagt
    17461 caggaagaga gagcactggt gcagctcaaa ccgtagggca gagataaatc actgcgagcc
    17521 atgccggaga aggaatgccg agtgtgccct gcgtcactgc aaatctctgg cattgaccgg
    17581 acagggaaga acttccgtcc gggggactat ggcgcggagg ggtagggctg ctacgccaga
    17641 gcaggcgatc ctaccagccc ctgctggtgc gctctgataa ggagcttctg gcggttggag
    17701 acgccgagtt tatcgaaaat gctggtgagg tgatgccgga ctgtaatgct ggagatgcac
    17761 agccgatcgg caatgtcttt attagaaagg ccttcactga ttaatcgcac cacctcgcgt
    17821 tcccgttccg tcaagccgtc cgaccattta gtgggagcag gaggtggggg aggcgtggtt
    17881 gggatagccg ggatgtggct taattttaac ggagccgggc tgtggccgat cggcaatacg
    17941 acttccctgc ctgagtcggc aagatagttg atggtcgcaa tgagtacggc ggatggctgc
    18001 attttgagga cgatgccatc gaccccggag gcaagcgcct gccgggtgca ttcagtatca
    18061 tcgaacccgc tgagcaggat ggtcttgacc cccggtgcca ctcgcttgat cttctggatc
    18121 aagtccggcg ccgtcccgcc gatttcggta tcgagaatga caatgtgcgg ctgctcgttg
    18181 tcgagtatct cgtcaagatt gcctgcgtga gcagcctgcc caaccaaccg gatccatttc
    18241 tcgtcatcca cgatcttctg caggccgagc cggagaagat agttgttcgt gacgatggcg
    18301 accacggtgg tggtttgtac ttctacgatt ctgctcatcg atcctcccga gtgaaacgcg
    18361 ccccagcgag tcgcagggta cacatccaaa aagtctctac gtagttcatg aaacagctac
    18421 gcaacgagta ctaggtcgaa tgtgtgatgg tgtgtgccta catggcgcat attcgcacac
    18481 ctcttcctct tcaggcaatg agcaaatctc attagattgt tcgccgcaaa gcattgcagg
    18541 gattcggttt gcagggattg ctcattcttc atttgtggaa cgatcgaaat gttcaagatt
    18601 gtgcacgcgt gagtctacga gtgtagcagc tagtgatttt tctaggttct catgccgagg
    18661 gtcactagac agggatgcga aaacggagcg gaataaatac cagtattcgt tgtgctccgt
    18721 ttgtggccga acctcgttgc atcgatccgg cacaaaggtg ctgagcgcaa aaattggtag
    18781 ctctagaaga aaagcaccat tgtccgatgt caacacagtc agaaatcttt agaaggatga
    18841 acgcactgat gggactcaga gggcgaagct atgttgaaga caattcaggc cggttctcgc
    18901 aatgcacatt cgctgacgac agatgccgca gtcggactcc agtggtacgc catcagcacc
    18961 cgctccaggc acgagaagct ggtccgggat cgcctggccg gcatcggtat tgagccgttt
    19021 cttccgttgg tgaaaaaact gagtcagtgg acggatcgaa aggtctggac cgaaagcccg
    19081 ctcttttcag ggtattgttt cgctcgcttt tcgctgatga acagtcatgc cgtgcttcaa
    19141 acgcctggga ctgttcgaat cgtgggatcg ctcatgcctg aagccattcc tgatgaggag
    19201 gtggcggcga ttcagaagtt agcggagagc cggcgcgcat tcgagcgcca cgactatctg
    19261 accgaagggt catgggtaga agtagtgcga ggcccgttga cagggttgcg cggacaatta
    19321 cttaggaaag ctaatcagga ctgtcttgtt atccgtgtgc atctgattca gcaagcggcc
    19381 actgtccata tcgatatgag tgaagtgcaa ccagttgtcc aaagtctcgc ctagcgtcgc
    19441 actccgcccg actatctctc cgttgaggcc tcgtctggat tttctgaatg caactcaaac
    19501 cccgaaagcg ttcccggttt accaacgatg agagacagcg gatccttgct gcggggacca
    19561 actccgcggt caaggcggtt tgtaaggagt atgggatttc tcccagtacc ttctaccggt
    19621 ggagagccgg atcggccgca cataagagtg aggacggcgc ccgcttagat catcttgagt
    19681 cggagaatcg acggctgaag aggcaggtgg ccgagttgtg gctggactac actagtctca
    19741 gaaatgcgct tatcaacggt aaggggcgtg aatgttgagg gtcgaaaccg gtggtgtcgc
    19801 aaagagtcgt cgccgcctca gacggtggtc tgcagtcgcg tcgctgagga tattgacgga
    19861 cgtttgctct gggtgatgag aattgttctg gcgtgcaggt attaggtttt atccgtgaac
    19921 gtgtctttcc acagtagggt atctcggata gccgcgaagg cgattccgta cggcacgatc
    19981 tgcgttgcgc tcgtggtggc tctgaccgtt tttccttcat ctggtttcag tcagcaggtg
    20041 tcttcgccgt taccgacagg cactcgcagc ctttcgtttg gagggatggg atgggcgtgg
    20101 ttctctcagt ttgcaaaccc ggcgttggtg ggtgctctct acatgacgcc ggattggcct
    20161 aagaccggct actatcctgt ttacccggga gcgttttcca gacccatcac ggaggatggc
    20221 atcgcggtcg ggcccgcgag gcttcacccg attttgggag tggccgaaat atacacggac
    20281 aatgtgttcc ggaccaattc gaaccgacgc agtgattttg cgacgacact ggcacctgga
    20341 attcaagccg aactgccttt tgcgggccgt cacacctttg tggccgacta tcgcagcaac
    20401 attcaattgt tccatcagaa tccctcgaac aatgtgcagg atcaaaccgc gtctggacga
    20461 ttcaatttcg attttcccgg cggtttgcga cttgatgtgc agggcgagca caagttgggg
    20521 catgatccgc gaggctcggc cctagatacc cagaacgtcg aggtgaacaa atggaccgcg
    20581 aacagtgttc ttggtcgact ttcttacgat ggggcgactg ccgggatcag gctgaatctc
    20641 tcttcaacgc gctggacgta tctcaataat gagcagagta tctttcgaaa ccgattgagc
    20701 aattacgcgg gtgtgaccgt actgggcaac gtcacgagca agacggcgtt ggttgccaat
    20761 gtcgcggcca accaagaggt ctacgacgac agtaaaacgc tcaatagcac tacctacagt
    20821 gcgagtacgg ggcttcaatg gaacgtgtct gaaatgacca ccggagagat tttggccggg
    20881 tatcagcatc tgaagtttgc gaatgccgaa agcggacaga gtgtcgccgg gctccaattc
    20941 ccgcgaagca gcgactccta tggtaacttt tttttcgtga gcaacgtgtc ctggaagccg
    21001 acaccgtttc tgactctgta ccttcaagga tatcggagtt ttcagcaatc ggtggtcttg
    21061 aacacgttgt tctttacttc gaccggagcc aacttttccg ccgtccatac gttgacgaat
    21121 agcaccgcgc tgaccttgaa tgtcggggtc gaacgcgaca aattcgaaaa tccggcaccg
    21181 actcctgaca atccgaaccg ggttgatctg atcaagaatg tggcgtttgg ggttcggtat
    21241 cgcacggtca agtgggccgg ggttgcgttg cagtatattt ttgaagatcg aagctcgaac
    21301 gtggatcgct tcaattatta tgcaaattcg gtcatgctgt cgcttcaagc cacgttctag
    21361 cgccgtgagg tcttgccgcc ctacgtttca cgagacggtg gatgtagacg ctcttggtcc
    21421 ttacggacct gttccgccga cgtgctgagg tcgtacgccc ttgggaacgt atggtgacaa
    21481 agcgatgggt gcctcaaatg ggacggtatt gtcaaaggag agactgtgaa cgccaaagcc
    21541 gttgtattgg atgaagccga gcagacgctc agtgagtacg ctgtatcctg ttggcggcat
    21601 cgctggttga ttgtgcttgt tgccggaatt tttggattcg gggcggcggg ttggtctttt
    21661 cttcaagtcc cggtttatca ggcgaaggcg actgtagtga ttgaaaatcc aacccccggc
    21721 ggattggaca aggataagtc attttatccg gacaatagtc ctgagtactt tcaaacccat
    21781 ttcgaattga tgaagagtca ttttgtgttg caacggacgg ccaggctgct ggaattgtcg
    21841 gaccgtcctg agtatcaacc caagccgtct gcgatcaggc aatttcttaa gtccctgctg
    21901 ccggactcgg tgctcacctt tctgcgccca gcccaaaaag aaaaagccga ttccgtcgac
    21961 gacgcagagg aagccttaat caagcagttt gctcaggaca tcgaaatcat gccgattcgg
    22021 ggggcgcggc tagcgcatgt cactgcgaat gccattgatc cggagttggc tgctcagatc
    22081 gccaacacgc tcgtgtcggt gtacatcgag cgcaatcagg agctcagcac caattccaag
    22141 gaacaggccg cgcagtggtt caccggccac ctcgaagagt tgcggcaaaa ggtgcaaaac
    22201 tcccagcagg cactgtatct gtttcgcgcg aagcatgggt tgttgacggg ggaagaacga
    22261 aaaagtgtcg cggcccatac cctggcggag ttgaattcac aactggtcaa gacagaaatg
    22321 gccaaggcgg aagcacattc gcggttcctg cagattcgat ccgtgctgaa tccgcaaggc
    22381 gcgggagccg caggggtaga ttggtcgaag ttggactccc agattcaagt cctcaattcg
    22441 cccttgattc agacgctccg ggcgcaggaa atcaccgtct cggcgcaggt ggcggagctg
    22501 tcggaaaagt atgggccgct acatcctaaa ttggctcaca tgaaagcgga gttgcaggac
    22561 ctccggcagc gcatccagca ggaagtccaa aaaatatacg attccgtcaa gcatcagtat
    22621 gacatggcct tggcgcagga caaagccgtg aaggaggcca tcacccggta cagcaccgat
    22681 aagatccgtc tggagcagaa cgaaatcgag cacagcatgt tggaacgcga tgcagaaacg
    22741 agccaacatc tgtacgatgt gttcctcaag gcgaccaagg aaacggatct gtcgtcgggg
    22801 ctgcgggcca acaacgtgta cctggccgat ccggccgccc caagttcggt accttccaag
    22861 ccgaggacca agctcaacat catgttggga ctcctggccg gattgatgag tggcgttggt
    22921 ttggcgattt tcgtcgacgg ccgaagcaag aaactcatgg cgcctgccga tgtcgaacgc
    22981 tatcttccga acgtgtcctt actcggggtt gtgccgttgc tctccaaagc tgcagctcag
    23041 gagaaggcct tggcgccgcc caatgcgctg acacccatcg ccgaaagctt ccgcattatt
    23101 cgaactagtc tcctcttgtc taaccccgac atgttgccct cgcgcatcct catcacgagt
    23161 ccgggggaga atgaaggcaa gaccacgctc gcggtcaatc tggcaatggc gatggctcag
    23221 ttggaggata cgcaggtggt cctgatcgat gtggactttc gcaaccagca catccatcct
    23281 ctgtacgatg tcgggagtcc ggagcaaccg ccaaagggac tggggcatct gttgcgaggc
    23341 gaggcggtct tggccgaggt agtgcacccg acgttcctca gcaacctctc cgtcattccg
    23401 cacggaggac gtcctgcgaa tccgacggag ctgctacact ccaaatccat ggcccagttg
    23461 ctgaattggg gcgccacaaa tcactaccac atcatcctgg actgtccccc ggtccttccc
    23521 atcgccgatg ctgcggtgtt ggcatctaaa gtagatggtg tcctgttggt tgtcagcgcg
    23581 ggtgaaacca cgcgtgatgc ctgtcacctt gccatacagc gaattaagca agccggcggg
    23641 cggctgctgg gagtggtgat gcaaaaagca cccgtggcgg actcatccta ttactacggg
    23701 gcctaccagc aacagcgttg aatattgcgg gagtgttatt cggcgtcagg cttcgtccgc
    23761 cgcggaccat cacaaaaata tgaatcacag tcttcggatc gggttgaagc ggtggggcac
    23821 atggcagcgt caagcatcca atcgacgggt gttagccgct ttgttcacag taggggggta
    23881 ctcgcttctc ggcaaggtgt cggccttcgc caaggacgcg gtcgttgcgt atcaatttgg
    23941 gcgtggcgac gagttggacg cgttcctgat tgcgctggtc attccccaat tcactattac
    24001 attgctcggc ggatcgctga atgcagcttt gattccgacc tacattcgag tccgagagca
    24061 ggaggggccg gaagctgcgc aacgagtctt ctcgacggtc acactgttga ccagcggctt
    24121 tcttctcctg acctgcttca tactcatgtt atccgcccca tggctgatgc ccattttggc
    24181 cgcaggatac ggtcccgaga agcttgtctt ggcgaaggtc ttgtatggag tcctcttgag
    24241 tacggtgcta ttcagcggga ttgggactac ctggggtgcg gtattgaacg cgggaaatcg
    24301 gtttgcttgg gccgcggccg ttcccatggt gacgtccctc acgacgatgg tggccgtgct
    24361 gtgtttggct cgctcctggt cggtgtatgc cctggcggct gcagccgtga cgggagcgtt
    24421 cgtcga
//
LOCUS       DAORRQ010000057        24277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_120_length_24277_cov_142.425605, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000057 DAORRQ010000000
VERSION     DAORRQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24277
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120_length_24277_cov_142.425605"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PK224_15660"
     CDS             complement(<1..583)
                     /locus_tag="PK224_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB17231.1"
                     /translation="MRIVALFLSSVLLGACAIGPDYSRPDLAVPDRFRMAATQQETES
                     FANLPWWDLLQDEELRRLIRVALAENKDLKRAVASVEDFQARMLIANMDFAPKADLTS
                     NAPLFGRKANFLFPGFPNPFNYYLQGNLSWEIDIWGRIRRSNEAARGDLLSREEARRA
                     VVLQLVSGVAEAYFDMLQFDMQLAVAQRTLKSWD"
     gene            complement(596..907)
                     /locus_tag="PK224_15665"
     CDS             complement(596..907)
                     /locus_tag="PK224_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17232.1"
                     /translation="MLKITSEQTSDSARLTLEGSLSGPWVTELERVWQQVKQAGTFAP
                     VVELTGITFIAEEGRALLTSMWREGAVLVANGCCTRHIVQEITGAGGGQAASSVRCET
                     T"
     gene            complement(949..2790)
                     /locus_tag="PK224_15670"
     CDS             complement(949..2790)
                     /locus_tag="PK224_15670"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB17233.1"
                     /translation="MREGKSRIQAALIGSRELVGPIIAMTITLAAVYAPIGFQGGLTG
                     SLFLEFAITLAAAVVLSGVVAITLSPVMSSRFVHPEGKEGKLTRLVNRGFDATRAVYG
                     RLLDGALEMRWAIVVASLLIMVAAVPLYLFSRQELAPVEDQSHISLFLEASPDSTVAA
                     TNSDSLKVVEAIRAFPEARFMWSLTSSWGGFGGLVAKDWRERTRSTELMYGEVFGAVS
                     QIPGLRVFPRLDPPLPTPGQYDVELILASEVPAEQLLEMTAAVLGAGWQSGKFLYVDT
                     DLKIDLPEARVVLDRERLADLGFDLAGVGQELGTLLGGAYVNRFNFYDRSYKVIPQIG
                     DKDRATLDPLLDLKIKTPSGQLVPVSTFTHIETSTAPRTLNRFQQRNAVRIFGGVKPG
                     VTKEEGLRVLETAAASAVGPRVALDFAGESRQIRHEGSALTVTLGFAVVLIYLVLAAQ
                     FKSFRDPLIVLLGSVPLAISGALVFSFLDLTTINIYSQVGLITLVGLIAKNGILIVEF
                     ANTLQERGLSKTAALREASLTRLRPVLMTSAATVFGHLPLVLVSGPGAAARNSIGMVL
                     VTGMTVGTIFTLFVVPVFYSLIAARHQPALELDAAEEGELQLAAAKG"
     gene            2803..3708
                     /locus_tag="PK224_15675"
     CDS             2803..3708
                     /locus_tag="PK224_15675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRB17234.1"
                     /translation="MQLAVAQRTLKSWDESVRIAEARLREGMISKIDADQFAAERANA
                     AARAAELSRQMVQKENQLSVLLGRVPGQIPRGRSLTDQVMPPEVPAGLPSELLQRRPD
                     LVQAEQELAAATARIGVAKADRFPKLSITGILGVASPQLSRLVANETAFGTAGPGLAG
                     PLLNAQILGFQQDAAEAQSREALARYEQALLVAFREVEDSLVAVRTVREQRNAQVEQV
                     DALRSALRLANLRYKGGLANYLDVLVAQRNLFEAELALTSTHRLQLVSVVQLYKALGG
                     GWSPLDTAQQQPGQAPAEPGKAGRG"
     gene            3754..4311
                     /locus_tag="PK224_15680"
     CDS             3754..4311
                     /locus_tag="PK224_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB17235.1"
                     /translation="MSQEDREAGPLAQFLAEDHRRLDALLQSAATQPAQVNREAYDQF
                     RAGLLRHIGMEEKILFPAVQRWRGGTPLPIAAKLRLDHGALATLLTPTPTPAILATIR
                     RLLTDHNAVEEGPGGVYDLCDQLAGFDAESLLKDLRAAPSVAVMPHSDSPAVMTTLRR
                     TLERAGYRMGAEIGRDVPVTPPQGS"
     gene            4330..5430
                     /locus_tag="PK224_15685"
     CDS             4330..5430
                     /locus_tag="PK224_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17236.1"
                     /translation="MVVRHPHRLPLLALGMLALLTGLWAGLARLGWDVPLPRPGFSSL
                     HGPLMVSGFLGTLISLERAVALGRPWAYAAPLLTGLGGVSLIVGAPLLVAQWLMLAGS
                     LGLVAIFAAIIRRHPALYTFTMGGGSLVWALGNLLWCLGWPVFLVVFWWAAFLVVTIA
                     GERLELARLQQVTGAAQATFLLLLGILLTGLLLLGWSFDGGVRLFGLGLAGLALWLGR
                     HDIARRTVKQAGLTRFIAICLLTGYIWLGISGLSAMWFGGVPVGPQYDATLHAFFLGF
                     VFAMIFAHAPIIFPAVLGARIAYRPLFYAHVALLQVTLVARVIGDVSGWGAGRQVGGL
                     LNAVTLLLFLVNTVSALQSPPERAGTTQARPS"
     gene            5478..5807
                     /locus_tag="PK224_15690"
     CDS             5478..5807
                     /locus_tag="PK224_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRB17237.1"
                     /translation="MTAENQRAGSDPRVLDALRQVVDPELGINIVDLGLVYGSEVRDG
                     QVHVAMTMTTPACPMEELLMEMVHAAILRELPEARSVEVDLVWEPAWKPEMMSPSAKA
                     QLGRTEG"
     gene            5838..7121
                     /locus_tag="PK224_15695"
     CDS             5838..7121
                     /locus_tag="PK224_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB17238.1"
                     /translation="MRLKGVPAVSEDQSRQVRELVESLYRSDSRQVLATLIRLLGDFD
                     TAEEALHDAFAVAVEQWAREGAPANPRAWLVSTGRFKAIDGIRRRARFDASLSELAKH
                     LELSTSNPEDWSDEAVEDDRLRLIFTCCHPALSPETQVAMTLREVCGLTTDEIARAFL
                     SKPATIAQRIVRAKGKIRDARIPYEVPSAADLPDRLDAVLRVVYLVFNEGYSASSGDS
                     LTRHDLSGEAIRLGRLLIGLLPEPEALGLLAVMLLHDSRRAARTSPTGDLILLEQQDR
                     SLWNRAQIAEGTALVERALASRQVGPYTIQAAIAAVHAEAPDAASTDWAQIVGLYDLL
                     VQAESSAVVELNRAVAVAMRDGPAAGLVLVDAILSRGELVNYHLAHAARADLCRRLGR
                     TAEACASYEKALSLTQQEPERRFLARRLAELSEVV"
     gene            complement(7179..7544)
                     /locus_tag="PK224_15700"
     CDS             complement(7179..7544)
                     /locus_tag="PK224_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB17239.1"
                     /translation="MNVTGIAFTCYPVTDLQRARRFYEGVLGLKESRFFGEGDKGFVE
                     YDIGANTLGIGNGAPDWKPSPGGGSVGLEVDDFAAAIARLKANGCPFRLEPLETPVCH
                     MAIVSDPDGNSIIIHRRKS"
     gene            7783..8244
                     /locus_tag="PK224_15705"
     CDS             7783..8244
                     /locus_tag="PK224_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17240.1"
                     /translation="MAEIDTTRWPGYSLCEPWLVASFSCLAVEGEHFVPANLILIGVV
                     LFLMPSAAKAEFSLEQYRSLIQSAEVKDKLSDYVTGVGRGIFWANVMLGADNKERLFC
                     LPAKLALDAGIIQSVLAQELRMSSPDATYEDDTPIELILTKAFMRRFPCDR"
     gene            8327..9259
                     /locus_tag="PK224_15710"
     CDS             8327..9259
                     /locus_tag="PK224_15710"
                     /inference="COORDINATES: protein motif:HMM:NF017953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRB17241.1"
                     /translation="MPFPIDKKLVVAVSSTALFDLASEHDLYLELGVDEFRKYQREHR
                     CTIPRRGAAFPFIKRLLNLNKIYSEEHPIEVVILSRNHADAGLRVMDAVSHYGLEISR
                     SFFLAGQLPYPYMRSIGAVLYLSTNREEVKTAVESGYAAGYVLPCDTIPSDEDQQLRI
                     AFDFDGVIASDEAEAVYKESGNLDLFHEHERKLKDKPLHHGPLMPLLLKISEFQKLER
                     KKAQSMAEYTQMLRIAIVTARNAPSHERLIHTLSEAGIDADELFLLGGIEKKLVLDVL
                     KPHIFFDDQIGHLEPASVSTPSVHVPFGAANFGG"
     gene            complement(9552..10247)
                     /locus_tag="PK224_15715"
     CDS             complement(9552..10247)
                     /locus_tag="PK224_15715"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HRB17242.1"
                     /translation="MVSRTYTDFKAFLDVFEKRQKDNYFQLFRGQPCNKSLLPKIARK
                     DPTKDTAQKEREMLAELRRRGTLFIGKELTDDWDLMVYAQHFGMATRLLDWTSNPLTA
                     LWFACQDGDTSNSGYVYLLNVGTDLLLDRGTQKDPFAIARTRVFKPNLNNNRIVAQSG
                     WFTAHRYSKSATRFVALERNPDVTRELIQIEIPGGLKYSVIKTLDVLGVNNQSLFADV
                     EGVCKHINWLHKA"
     gene            10480..10977
                     /locus_tag="PK224_15720"
     CDS             10480..10977
                     /locus_tag="PK224_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB17243.1"
                     /translation="MTEKNSPSILPDSDDYLVSRDGEQKEVMVATRKKKRAAKKPRAA
                     ASIVWFEVPADDLERAKKFYGSLFGWNFAKIPAAIDDYWHIDTGGKDATPDGGLMPRM
                     HPQQSITNYVGVPSVTMAMKKVEKLGGTICTPKTAVPRMGFFAICQDTEGNTFALWEM
                     NERAK"
     gene            11153..11509
                     /locus_tag="PK224_15725"
     CDS             11153..11509
                     /locus_tag="PK224_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRB17244.1"
                     /translation="MKYLCLVYVEEKTLNALSRDERVALSDESMAYCDGLQKNGQLLG
                     ASPLHPVEAATTVRVRDGKVSTTDGPFAETKEQLGGYLLIDVRDLNDAVRIASKFPAA
                     QYGSIEVRPIKEGGCA"
     gene            11561..11920
                     /locus_tag="PK224_15730"
     CDS             11561..11920
                     /locus_tag="PK224_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRB17245.1"
                     /translation="MKFILLVHHDETAFEKMSDETKKGLLTESIGLCHQLDAKGEYVH
                     ASPVHPAATATLVRVRDGRSLVTDGPFIETREQMAGYFLIEAKDRDEAVAIAAKVPGA
                     RIGTVEVRPVREITGLP"
     gene            12012..13193
                     /locus_tag="PK224_15735"
     CDS             12012..13193
                     /locus_tag="PK224_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRB17246.1"
                     /translation="MSRVRVLVGTKKGAFILTSDGTRKQWDVQGPYFGGWEMYHLKAS
                     PADPDRLYASQTSSWFGQVIQRSDDGGKTWNPPGTKPEDLMGTDGMPNGASNMFVYDA
                     SAETGKPLTTHQHYDGTQRPWEFKRIWHLEPSLTDPDTVYAGAEDAAIFKSSDGGKTW
                     NEMPGLRSAKGHLWQPGAGGMCLHTILLDQSQPDRMYIAISAAGAFRTDDGGKTWKPI
                     NRGLKSQYELPDPDAEVGHCVHRIAMHPSRPNVLFMQKHWDVLRSDDAGDSWHEVSGN
                     LPSDFGFPIAVHAHEPNTVYVVPIKSDSEHYPPEGKLRVYRSKTGGHEWEALTKGLPQ
                     QDCYVNILRDAMAVDQLEPCGLYFGTTGGQVYASRDGGDSWTAIVRDLPGVLSVEVQA
                     L"
     gene            13433..13729
                     /locus_tag="PK224_15740"
     CDS             13433..13729
                     /locus_tag="PK224_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRB17247.1"
                     /translation="MIRVVLPAHLRTLARVTGEVALDVASPVTQRAVLDALEARYPML
                     RGTIRDHVTHKRRPFIRFFACEQDLSHESADVPLPDTVASGTEPLLIVGAMAGG"
     gene            13858..14343
                     /locus_tag="PK224_15745"
     CDS             13858..14343
                     /locus_tag="PK224_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB17248.1"
                     /translation="MQKIAPCLWFDDQAEEAAKFYVATFRKAKLGPITYYGEAGATVS
                     GRPKGSVMTVAFEIDGQEFLALNGGPIFQFTEAVSLMVKCETQEEIDHLWEALSQGGE
                     KGQCGWLKDKYGLSWQIVVPQWDEMLRDKDAARSERVMAEILSMTKPDLQCVQRAYEG
                     R"
     gene            14371..14565
                     /locus_tag="PK224_15750"
     CDS             14371..14565
                     /locus_tag="PK224_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17249.1"
                     /translation="MGVKLIWGGRVMSGLLAGLLTMSAAMKLIGGRKVIEGVARMGLP
                     ESLRPPENLAPCRFWAGAVD"
     gene            14636..15064
                     /locus_tag="PK224_15755"
     CDS             14636..15064
                     /locus_tag="PK224_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRB17250.1"
                     /translation="MRFIVFVKATKNSEAGVMPSQELLSAMMKYNEELVQAGVMLGGE
                     GLHPSSKGVRVKFSGSKRIVTDGPFVETNEIVAGYWLWQCKSKEEAIEWVKRCPSPMP
                     GEESEIEIRPLFEADDFGAELTPELREREEQLRAQAAGKQ"
     gene            15259..15858
                     /locus_tag="PK224_15760"
     CDS             15259..15858
                     /locus_tag="PK224_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HRB17251.1"
                     /translation="MRYITVTLVALALVMTASFGEAKEKKHDKQMDPQAMMELWQKLA
                     QPGEPHKAFAGLAGTWTTTTKEWMEPGKPPTEASGTADMKMLLGGRFLYQEFNSQMMG
                     QPFSGIGIDAYDNVRKKYVTAWMDTMGTGIFIMEGTASADGKTITLKGSHPEPGGGQM
                     HHRAVWKLVDSNTQTFEMYGNHGHGKEEKMMEITYTRKP"
     gene            15995..16429
                     /locus_tag="PK224_15765"
     CDS             15995..16429
                     /locus_tag="PK224_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17252.1"
                     /translation="MQRTIGFLAMALILTSLSGCSYLFYPRAGDYATQAKGANGLETM
                     TNLTSMMESTASKAKGGKGVDMAFDDLHNQFHALSDSFCGVTDAQSKTPAYALAVTHK
                     KELSAIFKRLWKFKDDQPQRDQHLELLATELKELRETLRTIK"
     gene            16878..18074
                     /locus_tag="PK224_15770"
     CDS             16878..18074
                     /locus_tag="PK224_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17253.1"
                     /translation="MARRSRFSTVAPMIYGCLALALLSSCKQEVGSSQAPPVPEVGIV
                     VATAQDVPDEPEFIGQSESSRPVEIRSQVTGILKEWFFKEGRDVKKGDRLYQIDPVPF
                     HAAMLSAKAKVAQAEARLVQAKQNLARVKPLLAEQAVSTKDVDDAVAEEMAAKANLEG
                     AKAELVKAKFDLDNTLIVAPISGMIERTRVYEGRLVSAQTDLLTIIQQVDPMYVIVSA
                     PESFLLKRQRDSTAKRIQHPGVYQLRGVLTFADGTTYGQEGVLDLLDVGLKTDTGSRQ
                     ARVVFPNHDRVLLPGQFVRVRFKGTVKTGAILVPQRAVQQGAKGSIVFVVGNEDKVEM
                     REVQATSWQGNQWIVEQGLHVGDRIIVEGLHKIAPGASVKPVPLPAPAATTVPTAAKP
                     QPERAS"
     gene            18071..21250
                     /locus_tag="PK224_15775"
     CDS             18071..21250
                     /locus_tag="PK224_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRB17254.1"
                     /translation="MISHFFIDRPIFASVLSIVIMVVGLVALKALPVAQFPEITPPVV
                     QIEADYPGANAEIVADSVARPIEVQLPGIDNLLYYDSTSTNDGHMSIKLTFEIGTDVD
                     IAQVQTQNRVKLAEPQLPPEVVRQGISINKVSPDLLAVVALSSIDPTHDTVYLSNYAI
                     LRVLDNVKRLRGVGNAVVFGSQNYSMRLILDPIRMAQLSLTPTDIANVVREQNRDFPA
                     GTIGREPALKGTELTIPVITQGRLTEVKEFEELIVRAMPNGSMIHLKDVARVELGAQS
                     YTLEGRWNGKPNVFLLTFLSPGANALETVKRVRAELAEVSKSFPTGVSYDIPYDTTRF
                     IDVSIKEVVKTLAEAMVLVILVVYLFLQSWRATLIPGVAVPVSLVGTFAGMQALGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENCERHMTQGRLSAKNAAKRAMEEVTGPVIAIVLV
                     LCAVFVPVGFLGGITGELYKQFAITISIAVIISGFVALTLSPALCALVLKPGEEQHHG
                     IFGLFNRFFSWMQLRYISTVGLALTRRVLSVAVFAVLLIGVFMLFRVIPSSFLPEEDQ
                     GYFITIVQLPDGASKQRTDAVLSKIESYFLASPVIHSTDALSGQNFVFNTRGPNAATM
                     FVPLKHWDERSAPQHHVKALIGAAYGEFAKIPEALILAFNAPSIRGLGATGGFTLQLQ
                     DPSSGDFNKFSAAAQEFVGKARQNPAIGAIGTSFRVSAPRILATVNRERAKALGVPIS
                     EVFDTMQAYFGNFYINDFIKFGRVFRVQTEADAQYRSTPEDISKIYVRASGPQGTTMI
                     PLDTVVSTEFTSGPDPVTHFNGYNTALVLGAAAPGFSSGQVLDALEQVAKEVLVPQGY
                     GIDWSGISYQERMVGSQSLYAFAFGLLMVFLVLAAQYESWVVPFAVILAVPIGLFGAL
                     SAVWLKGMTNDIYFQIGLVTLIGLSAKNAILIVEFANKRFEEGHPLLEATIEAAKLRF
                     RPIVMTSMAFILGVVPLVIATGAGAASRNSIGTGVFGGMLAATFLAIFFVPLFFVLIR
                     SLSRRLKKETPPAQDAPDNPEKERDYQHA"
     gene            21243..22679
                     /locus_tag="PK224_15780"
     CDS             21243..22679
                     /locus_tag="PK224_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRB17255.1"
                     /translation="MRRLALVLSSTLLTACAVGPDFTRPDATTPDAFRMAEAGGNAAS
                     IANTPWWELLKDQELQKLIRTALEENKDLQRAAAAVEEFQARLFIAKTDFAPQMNVTS
                     NAPLFGRKTNFLFPGFPNPFNYYLQGNLSWEIDIWGRIRRSNEAARGDLLSREESRRA
                     IVLQLVSGVAEAYFELLQFDMQLDIARRTLKSWEESVRIAQARLRQGMISKLDADQFE
                     AERANAAAKAAEFERQKVQKENQLSVLLGRNPGRIARGHSLTEQVMPPDVPPGLPSEL
                     LQRRPDILQAEQDLAAATARIGMAKADRFPKLSITGILGVASPHLSRLVANETAFGVA
                     GPGFAGPLLNAQILGFQQKAAEAQARQAVAQYEQSVLVAFKEVEDSLVAVSTVREQRT
                     AQLQQVDALRSALSLANLRYKGGLANYLDVLIAQRNLFEAELALMGTHRLHLVSIVQL
                     YKALGGGWTPEGRPPVQALPLVSTQETR"
     gene            complement(22849..23220)
                     /locus_tag="PK224_15785"
     CDS             complement(22849..23220)
                     /locus_tag="PK224_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17256.1"
                     /translation="MTSTAKTVVRDAAIVYGLTFAAGLCMAAAGITLENNSSTAYLCN
                     LLSGVLGFTLVGTRLSANRAEHLAWVAATLWTFNLTNIVLGLQTSSAWIHSGLTILLM
                     ASLGGSLAMILTLTSAADRRT"
     gene            complement(23811..24056)
                     /locus_tag="PK224_15790"
     CDS             complement(23811..24056)
                     /locus_tag="PK224_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17257.1"
                     /translation="MTCSRCNGCMVEDFLLDMEDSSGPMWLQAKRCMNCGNVAEKVLE
                     HNRQTQGAALHSSKSAPTIPNSEHAHFGSGGMAQLAA"
     gene            complement(24082..>24277)
                     /locus_tag="PK224_15795"
     CDS             complement(24082..>24277)
                     /locus_tag="PK224_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17258.1"
                     /translation="AGIVLLKKVGMMPSGFHLRASRRAKTRCTYQYLATSKNIPPGGT
                     TKEVLHSGLSEMTIRFYAYT"
BASE COUNT         4957 a         6615 c         7556 g         5149 t
ORIGIN      
        1 cgtcccagga tttcagcgtt cgctgcgcga cggccaactg catgtcgaac tgcagcatgt
       61 cgaagtaggc ctccgccacg ccgctcacca gttgcaagac aaccgcgcgg cgagcctcct
      121 cccgcgacag gagatctccc cgcgccgctt cgttcgatcg acgaatccgt ccccagatat
      181 cgatctccca ggagagattc ccctggagat agtagttgaa cggattggga aagcccggaa
      241 acagaaagtt tgccttgcgg ccgaacaagg gcgcgttgga cgtgagatcg gccttcggcg
      301 cgaaatccat atttgcgatc aacatgcggg cctgaaaatc ttcgaccgac gcaaccgccc
      361 gtttcagatc tttattctca gccagcgcca ctcgaataag acgccgcagt tcttcatcct
      421 gcaacagatc ccaccagggc aggttggcaa acgactcggt ttcctgctgg gtcgccgcca
      481 tgcggaaccg gtccggcaca gcaaggtcgg gacgggagta gtccggcccg atggcacagg
      541 cgcctaggag aacagagctt aggaagagag ctacaatgcg catgcgatga ccgtcctatg
      601 tcgtctcaca gcggacggat gaggcagcct ggccgcctcc cgccccggtg atttcttgca
      661 cgatatgtct ggtgcagcac ccgttcgcaa ccaggacagc accctcccgc cacatgctgg
      721 tcagcagagc cctgccctcc tcggcaatga acgtgattcc tgtcagttcc accacggggg
      781 caaacgtgcc ggcttgtttg acctgctgcc acacccgttc gagttcagtc acccagggtc
      841 cgctcagact cccttccaag gtcagacgcg cggaatcgga agtctgctcg gacgtaatct
      901 tcagcatcgt gcatgtcctc ctgttgcgct caacgagccg ggatcagttc atccctttgc
      961 cgccgccaac tgcaactcac cctcttcggc cgcgtccagt tctagcgccg gctgatgtcg
     1021 ggccgcgatc agcgagtaaa acaccggcac gacgaacagc gtgaagatgg ttccgaccgt
     1081 catccccgtg accaacacca tgccgatgct gttgcgggcc gcggcgccgg gtcccgaaac
     1141 gagcaccagc gggaggtgcc cgaagaccgt ggcggccgag gtcatcagga ccgggcgcaa
     1201 tcgcgtcagg gatgcttcgc gcagggcggc cgtcttcgac agccctcgtt cctgcagagt
     1261 attcgcgaac tccacgatca aaatgccgtt cttcgcaatc agccccacca gtgtaatcaa
     1321 cccgacctgc gaatagatgt tgatcgtcgt caggtcgagg aaactgaaca ccagcgcacc
     1381 tgagatcgcc agcggcaccg agcccagcag gacgatcagc gggtcccgaa aacttttgaa
     1441 ctgagccgcg agcacgaggt aaatcagcac caccgcaaag cccagtgtca ccgtcaaggc
     1501 cgaaccttcg tggcggatct ggcgcgattc tcccgcaaaa tcgagcgcca cccgtggacc
     1561 gacggccgag gccgccgcag tttccagaac gcgcagccct tcctccttcg tcaccccggg
     1621 cttcaccccg ccgaagatcc gcaccgcatt acgttgttgg aaacggttca gcgtccgcgg
     1681 cgcagtactc gtctcaatat gcgtaaacgt cgagaccggc accagctggc cgcttggtgt
     1741 cttgatcttg agatcgagca gcgggtccaa cgtcgcgcga tccttgtcgc cgatttgagg
     1801 aatgaccttg taactgcggt cgtaaaaatt gaaccgattc acataggccc cgccgagcag
     1861 cgtccccagt tcctgaccga ccccggccag atcgaagccc aggtcggcga gtcgctcgcg
     1921 atcgagcacg acccgcgctt caggcaaatc aatcttcagg tcggtgtcga catacaggaa
     1981 cttgccgctc tgccaaccgg cacccaggac cgcggcggtc atttccaaca gctgctcggc
     2041 aggcacttca ctcgccagga tcagttccac atcatattgc ccgggcgtcg gcaatggcgg
     2101 atcaaggcgc gggaacaccc gcagaccggg gatctgcgac acggctccga agacttcgcc
     2161 gtacatcagc tcggtcgagc gcgtccgctc ccgccagtcc ttggccacca ggccgccgaa
     2221 gccgccccac gacgaggtca acgaccacat gaagcgggct tccggaaacg cccggatcgc
     2281 ttcgacaacc ttcaaggaat cactgttcgt agcagccacc gtcgagtccg gcgacgcctc
     2341 cagaaagaga ctgatatgac tctggtcctc cacaggcgcc aattcctgcc gtgaaaagag
     2401 atacagcgga acggccgcaa ccatgatcag caacgaggcc acgacaatcg cccagcgcat
     2461 ctccaacgcg ccgtcgagca accgcccgta gaccgcccgc gttgcatcga atccacggtt
     2521 gaccagacgg gtcaacttgc cctctttccc ctccgggtgg acgaaccgcg agctcatgac
     2581 cggcgagagc gtgatcgcca cgacgccgga cagcacgact gcggcggcca gggtgatggc
     2641 aaactccaga aacagcgagc cggtcagacc tccctgaaaa ccgatcggag cataaactgc
     2701 ggccagggta atggtcatgg cgatgatggg cccgaccaat tcgcgcgagc cgatcaacgc
     2761 cgcttgaatc cgcgactttc cttcgcgcac atgcagttcg acatgcagtt ggccgtcgcg
     2821 cagcgaacgc tgaaatcctg ggacgaatcg gtcaggattg ccgaggcccg gctgcgggag
     2881 ggcatgatct ccaaaatcga cgcggaccag tttgcggcgg aacgcgccaa cgctgcggca
     2941 cgggcggcag agttgagccg acagatggtg cagaaagaaa accagctcag tgtgttgctg
     3001 gggcgtgtcc cgggtcagat tccgagagga cgttctctca ccgatcaggt gatgccaccg
     3061 gaggtgccgg ccggccttcc ttcggaattg ctgcagcgcc gcccggatct cgtgcaggca
     3121 gaacaagaat tggcggcggc cacggcgcga atcggtgtgg cgaaggcgga ccggtttccg
     3181 aaactcagca ttacgggaat tcttggtgtc gccagtccgc agttgtcccg cctggtggcg
     3241 aatgagacgg cgttcggtac cgcaggtccc gggcttgccg gtcccttgct gaatgctcag
     3301 atcctcgggt ttcagcagga cgcggctgaa gcgcagagcc gtgaagcgct tgcacggtat
     3361 gagcaggcgt tgctggtggc ctttcgtgaa gtggaggatt cgctggtcgc cgtgcgaacg
     3421 gtgcgggagc agcggaatgc gcaggttgag caggtggacg cgttgcgttc ggctctgcgt
     3481 ctggccaatc tgcggtataa aggaggactc gcgaactatc tcgacgtcct cgtggcgcaa
     3541 cgcaatctgt tcgaggcgga actggcgtta accagcaccc accggttaca gctggtgtcg
     3601 gttgtgcaat tgtataaagc gctagggggc ggatggtcgc cgctcgatac ggcccagcaa
     3661 cagccgggcc aagctccggc agaacccggg aaggcggggc gcggctgacc cacggacccg
     3721 ggggaaagag ggcgacatga cgggagccgg ggcatgagcc aggaggacag ggaggcaggc
     3781 cctctcgcgc aattcctcgc cgaagaccat cggcggctcg acgcattgtt gcagtcggcg
     3841 gcgacccaac cggcccaggt caatcgcgag gcctacgatc aatttcgtgc cggactgctc
     3901 cggcacatcg gcatggagga gaaaattctg ttccctgccg ttcagcgatg gcgtggcggc
     3961 acaccgcttc cgatcgctgc caagcttcga ctcgatcacg gcgcgctggc cacgttgctc
     4021 acgcccactc ccacccctgc gatcctggcg accatccggc gtctgcttac ggaccacaat
     4081 gctgtcgagg aaggaccggg cggcgtctac gatctctgtg atcaactggc cggcttcgat
     4141 gccgagtcac tgctgaagga cctgcgcgct gctccgtcgg tcgccgtgat gccccattcc
     4201 gacagccctg cggtgatgac tacgctacgc cgcacattag aacgggccgg ttatcgcatg
     4261 ggcgcggaga tcggtagaga tgtgccggtg actccgcctc agggatcctg acagaaagtt
     4321 cacccgtcta tggttgtgcg acatcctcac cgtcttccgc tcctggcgtt ggggatgctg
     4381 gcgttgctca ccggtctctg ggcggggctt gcgcggctcg ggtgggatgt gccgcttccg
     4441 cgacctggat tttcctcctt gcatgggccg ctcatggtgt ctggctttct gggtacgctg
     4501 atcagcttgg agcgtgcggt ggctctgggc cggccctggg cctatgccgc gccgctgctg
     4561 accggactcg gcggtgtgag cttgattgtc ggggccccgc ttcttgtggc gcaatggttg
     4621 atgcttgccg gcagcctcgg gctggttgcg atttttgcgg cgatcattcg ccggcacccg
     4681 gcgctgtaca cgttcaccat ggggggcgga tcgctagtct gggcgttggg caatctgctc
     4741 tggtgtctcg ggtggcccgt ctttctggtc gtgttctggt gggcagcgtt tctcgtggtg
     4801 accattgccg gtgagcgcct ggaactggcc cggctgcagc aagtgaccgg agccgcacag
     4861 gcgacgtttc tgctgttgct cggcattctg ctcacggggc tgttgctgct gggctggtcg
     4921 tttgacgggg gtgttcgtct gttcgggttg gggctggccg gtctagccct gtggttgggg
     4981 cggcatgaca ttgccaggcg aaccgttaag caagcagggc tgacgcggtt tatcgcgatc
     5041 tgtctgctga ccggctatat ctggctgggc atcagcgggt tgtccgccat gtggttcggc
     5101 ggtgttcccg tcggcccgca gtatgacgcg accctgcatg ccttttttct cgggttcgtg
     5161 tttgcgatga tctttgccca tgcgccgatc atctttccgg cggtgctcgg cgcaaggata
     5221 gcctatcggc cgctgttcta tgcgcatgtg gccctgttgc aggtgaccct tgttgcacgt
     5281 gttatcggcg atgtgagcgg gtggggcgcg ggacgccagg tcggtgggtt gctcaatgcg
     5341 gtgacgctgc tccttttcct cgtgaacacg gtgtctgcgc tgcagagtcc gcccgagcga
     5401 gcgggcacca cacaagccag gccgtcgtga cgtggttgga aaaatagaca gtgtttcgcc
     5461 ggggaaagga agttcgaatg acggcagaga atcaacgtgc tggttccgat ccgcgcgtgc
     5521 tcgatgccct gcggcaggtg gttgatcccg agttagggat caacatcgtg gatctcggat
     5581 tggtgtacgg cagcgaggtg cgggacggtc aggttcacgt ggccatgacg atgacgacgc
     5641 ctgcctgtcc gatggaagaa ctgttgatgg agatggtgca tgcggcgatt ctgcgcgagt
     5701 tgcccgaggc gcgttcagtg gaagtggatc tcgtgtggga gcccgcctgg aagccggaga
     5761 tgatgagtcc gtcagccaag gcgcagctgg gtcggacgga aggctgagat ggcgaatgca
     5821 ggaggctgca cgagcctgtg cggctgaagg gtgttccggc cgtgagcgaa gaccaatcca
     5881 gacaggtgcg cgagttggtg gagtcgctat atcgttcgga ctcccgccag gtgctcgcca
     5941 ccttgattcg tctgctgggg gacttcgata cggcggaaga ggccctgcac gatgcctttg
     6001 ccgtcgcggt ggagcaatgg gcgcgggagg gggcgccggc caatccaagg gcttggctcg
     6061 tctcgaccgg ccgcttcaag gccatcgacg ggatacgacg gcgcgcccgt ttcgacgctt
     6121 ccctgtcgga actcgccaaa cacctggaac tcagcaccag caatcccgaa gattggagcg
     6181 acgaggcggt cgaggatgac cggctgcggc tgatcttcac ctgctgccat ccggccttgt
     6241 cgcccgagac gcaggttgcg atgacgttac gcgaagtctg tggcctcacg acagatgaga
     6301 tcgcgcgcgc atttctcagc aagcctgcga ccatcgcgca acgcattgtg cgggccaaag
     6361 gcaagatccg tgacgcccgc attccctacg aggtgccgtc ggcggccgac ttgcctgacc
     6421 ggctggatgc ggtgttgcgg gtcgtgtacc tcgtctttaa tgaggggtac agcgcttcgt
     6481 cgggggactc gctcacgcgg cacgatctgt ccggggaggc gattcgcttg gggcggctgc
     6541 tgatcgggtt actgccggag cccgaagccc tgggccttct ggcggtgatg ctgctgcacg
     6601 actcgcgtcg agcggcacgc acctcgccga ccggcgatct gattctcctg gagcagcagg
     6661 atcgctcgct gtggaaccgg gcgcagatag ccgaaggaac ggccttagtc gagcgggcgc
     6721 tcgcgtcgcg tcaggtcggt ccctatacca ttcaggccgc gattgcggcc gtgcatgcgg
     6781 aggcccccga cgccgcatcg accgactggg cgcagatcgt cggtctctac gatttattgg
     6841 tgcaggctga gtcgtcagcc gtggtcgagc tgaaccgggc ggtcgctgtg gcgatgcgcg
     6901 acggcccggc ggcggggctg gtcttggtcg atgcgattct ctcccgcggc gaactcgtga
     6961 actatcacct agcgcatgcc gcccgtgccg acctgtgccg gcggctcggg cgaaccgcag
     7021 aagcctgtgc ctcatacgag aaggccttga gcctcaccca gcaggaaccg gagcggaggt
     7081 ttctggcgcg acgcctggcc gaattaagcg aagtcgtcta gtagagcact ggacattatg
     7141 gtggttacca gggtgttctc cggtcgccga ctctctcgtc aggatttccg ccgatggata
     7201 atgatggaat tcccgtccgg gtcggagacg atcgccatgt gacaaacggg tgtctccagc
     7261 ggttcaagcc ggaacgggca tccgtttgcc ttgagccgcg cgatcgcggc agcgaaatca
     7321 tccacttcga gcccgaccga tccgccgccc ggggacggtt tccaatccgg tgcgccgttc
     7381 ccgataccga gcgtgttcgc cccgatgtcg tattcgacga accccttgtc tccctcgccg
     7441 aaaaaccgcg attctttaag ccccagcacg ccttcataaa accggcgcgc ccgctgaagg
     7501 tctgtgacgg ggtaacaagt aaatgcgatg ccggtgacat tcatgtcgat cctttcttcg
     7561 ttcgatcgtg tgtgcttatg ctgcacttca gttatgttgg ttgcaagggg ctgttcttgc
     7621 cgtggagggc tgtggtcgtt cgcactgctc tctgcggaga ttgaagcgaa tggcagaagc
     7681 tggaacggag gtttctaaag cggaggacga aggcgttaca gcatgactga gcctaaagat
     7741 ctatgacctg tgcactactg tgtcgcattc tcggaatatg ctttggcaga gattgacact
     7801 acaagatggc ctggctacag tttgtgcgaa ccatggctgg tggccagctt ttcctgtttg
     7861 gcggtggaag gggaacattt tgtgcctgca aatctaatcc tgataggtgt cgttctgttc
     7921 ttgatgcctt ccgcagctaa agcagagttc tctctggagc agtatcgttc ccttatacag
     7981 tctgccgagg tcaaggataa gctgagcgac tatgtgacag gggtggggcg cggtattttc
     8041 tgggcgaatg taatgctggg cgccgataat aaggaacgtc ttttttgtct gcccgccaag
     8101 cttgccctgg atgcaggtat tatccaatca gttcttgccc aagaactgag aatgtcatct
     8161 ccagatgcaa cctatgagga cgatacaccc atcgaattaa ttctgacgaa ggcgtttatg
     8221 cgtcgtttcc catgcgacag atagacgcca gccggagtag cccaccggtc ggcaatttcg
     8281 ctgggctaca ccgcgtttgt gcatcttggc ggtagccact aaatcaatgc cattcccaat
     8341 cgacaagaag ctagtggttg ccgtatcgtc cactgcgcta ttcgacttag cgtccgagca
     8401 tgacctatat ctggagctgg gcgtagatga gtttcgcaag tatcagcgtg agcatcgctg
     8461 tactattcca cgtcgcggcg cagcgttccc attcattaag cgcttgttga atttaaacaa
     8521 gatctacagt gaggagcatc caatagaggt cgtaatctta tctcgaaatc atgctgatgc
     8581 agggctgcgc gtaatggatg ccgtttccca ttacggtctt gagatctcac gttccttctt
     8641 cttggctggg cagctcccat atccatacat gaggtcgata ggggctgttt tatacctcag
     8701 caccaacaga gaggaagtga aaacggcggt tgagagcgga tatgctgcag ggtacgtgct
     8761 cccttgcgac acaataccgt ctgacgaaga tcaacaattg aggatcgctt tcgattttga
     8821 cggggtaatc gctagcgatg aggctgaggc cgtgtataag gagtcaggaa acctagatct
     8881 gtttcatgaa catgaaagaa agttaaagga caagccactg catcatgggc cactcatgcc
     8941 actgctcctt aagatttcgg aattccaaaa gttagagagg aagaaggctc agagcatggc
     9001 ggaatacacg cagatgctaa gaatcgcaat cgttaccgcg cggaatgccc cctctcatga
     9061 gcggctgatc catacgcttt cagaggcggg gattgacgcg gatgaactgt tcttgctcgg
     9121 tggtatagaa aagaagttag tgctagatgt attgaaaccg catatcttct ttgacgacca
     9181 gatagggcat ttagagcctg cctcagtatc aacgccatcg gttcatgttc cgtttggagc
     9241 cgccaacttc ggtgggtaac cacatggtcc gtccagggtt ccggcccggg caactttgat
     9301 gcccctggca gacgggccaa agtctgagtt cttaggagat caccgtagga aattgagtct
     9361 gggttgtgat aatggcggca gccgcaattt tgtttgcggc catcaagtag ttttccaggt
     9421 gcacattccg tgatccgata atgggaatcc accagttgaa aagctcgaag ccaaaaggat
     9481 ctggaaaatt cattcagtcc tgtgaaaccg agagattgag cgaggtagtc tgcgaggcac
     9541 tttgtgcgtg ctcacgcttt atgcagccag ttgatgtgtt tgcagacgcc ctcaacatcg
     9601 gcgaatagag actggttgtt cactcctaac acatcaaggg tcttgatgac gctgtattta
     9661 agtccaccgg gaatttctat ctgaattagc tccctagtta catcggggtt gcgttcgagg
     9721 gcaacgaagc gagttgcgct tttagaatat cggtgcgctg tgaaccatcc actctgagcg
     9781 acgattcggt tgttgttaag gtttggtttg aatacgcgcg ttcttgcaat agcgaaggga
     9841 tccttctgtg tgccgcggtc aagcaagagg tcagttccaa cgttgagaag atagacatat
     9901 cctgagttgc tggtatctcc gtcttgacag gcaaaccaga gagccgtcag aggattcgaa
     9961 gtccaatcca aaaggcgggt agccatgccg aagtgctgtg cgtaaaccat taggtcccag
    10021 tcatctgtaa gctccttacc gatgaacagg gtccctcgtc ggcggagttc cgcgagcatc
    10081 tcgcgttcct tctgcgctgt gtcctttgtg gggtcttttc tggcaatctt tggcagaagc
    10141 gacttgttac aaggctgacc tcgaaataac tgaaaatagt tgtctttttg gcgtttctca
    10201 aagacgtcca ggaacgcctt gaaatccgta taagtgcggc taaccataaa tctctacata
    10261 gtcacatgaa actaagggac ctctgtctgt gcgggatctt actcccatcc caaagctttt
    10321 caaactgcag aattcattga aatcatgacg ctttagtaac ctgtctcgat ttgacatgcg
    10381 gccactgatt ttttttcata aacccatgtc gatttcgtcc gttcatggcg actatctaat
    10441 gtcacgggct cgttaatcgc accttgcaga cgggtcgtcg tgaccgaaaa gaactccccg
    10501 tcgattttgc ccgactccga cgactatctt gtgtcacgag atggcgaaca gaaggaggtc
    10561 atggtggcga cgaggaagaa gaagcgggcg gcgaagaagc cacgggcggc ggctagtatc
    10621 gtctggtttg aggtgcctgc tgacgatctc gaacgggcga agaaatttta tggctcgttg
    10681 ttcgggtgga acttcgcgaa gattccagcg gctatcgacg actattggca cattgatacc
    10741 ggtggcaaag atgcgacccc cgacggcggg ctgatgccgc gcatgcatcc gcaacaatcc
    10801 atcaccaact atgtcggcgt gccgtcggtg acgatggcga tgaagaaggt tgagaaactc
    10861 ggtggaacga tctgcacgcc caagacggcg gtgccgcgca tgggtttttt cgcgatctgt
    10921 caggatacag agggcaatac ctttgcgcta tgggagatga acgagcgcgc caagtgagag
    10981 tgctgcaaaa gccctccagc gtcgttctca tatcgctcaa ggtctcgacg tacgaaacaa
    11041 cgtacgcctc ggcctttcgc tcactgcggc cttgctgggc tgactttttc agcattctca
    11101 atgcaagtcg taccccttta gcaccgtgag cccatttgag agaggagtgg tcatgaaata
    11161 tctctgcctg gtgtatgtcg aagaaaagac gttgaatgct ctgtcccgag atgagcgagt
    11221 cgcgttgtcg gacgaatcca tggcctactg tgatgggctg caaaaaaacg gtcaactgct
    11281 tggggcctca ccccttcatc cggtagaggc cgcgacgacg gtgcgggtgc gcgacgggaa
    11341 agtctccacg accgacgggc ccttcgcaga aacgaaggaa cagttgggcg ggtacctgtt
    11401 gatcgatgtt cgggacctca acgacgccgt acggatcgcc tcaaagtttc ccgccgctca
    11461 gtacggcagt atcgaagtgc ggccgattaa agagggcggt tgtgcctgac tgttcggtcg
    11521 acagaggtaa cggatcgacg agccttggtg gaggtgctgc atgaaattta tcttgctggt
    11581 gcatcacgac gaaacggcat tcgagaagat gagcgacgag acaaagaagg gcctactgac
    11641 ggagtcgatc gggctctgcc accaattgga tgcgaagggg gagtacgtgc atgcctctcc
    11701 ggttcacccg gctgccacgg cgaccctcgt gcgggtgcgg gacggtcgtt cgctggtgac
    11761 cgatggcccc ttcatcgaaa cgcgggagca gatggcgggc tattttctga tcgaggcgaa
    11821 agatcgcgat gaagccgttg cgatcgctgc gaaagttccc ggtgcgcgaa tcggaacggt
    11881 ggaagtgcgg cctgtgcggg agatcaccgg attgccgtga aacgtgagga gtgagcgctt
    11941 cagacgtttc acgagatacg aacgacgaga tacgcttcac gaaacactca tggttgagaa
    12001 aggagtcgga tatgagccgg gtacgggtac ttgtcgggac gaagaagggt gcgttcattc
    12061 tcacatcgga tggcacgcgg aaacagtggg atgtgcaggg gccgtacttc ggcggctggg
    12121 aaatgtacca tctgaaagcg tcgcctgccg atccggatcg gttgtatgcc tcgcagacca
    12181 gcagctggtt tgggcaggtg attcaacgtt cggatgatgg cggcaagacc tggaacccgc
    12241 cgggaaccaa accggaagat ttgatgggga cggacggaat gcccaacggc gcaagtaaca
    12301 tgttcgtcta tgacgcgtcg gcggaaacgg gcaaaccgtt gaccacccac cagcattacg
    12361 acggcacgca gcggccgtgg gaattcaaac gcatctggca cctggaaccc tcgctgaccg
    12421 atccggacac ggtctatgcc ggcgcggaag acgcggcaat tttcaaatcg tcggatgggg
    12481 gaaagacctg gaatgagatg ccgggcctgc gcagcgcgaa gggccatctc tggcagcccg
    12541 gcgccggggg gatgtgcctg cacaccattc tgttggatca gagccagccc gatcgcatgt
    12601 acatcgccat ctctgcggcg ggcgcattcc gcaccgatga tggcggcaag acctggaaac
    12661 cgattaatcg cggtctcaag tcgcaatatg agctgccgga tccggacgct gaagtcggcc
    12721 actgtgtgca tcgcattgcc atgcatccgt cgcgtccgaa cgtgctgttc atgcagaaac
    12781 attgggacgt ccttcgcagc gacgatgccg gcgactcgtg gcacgaggtc agcggcaacc
    12841 tgccgagcga tttcgggttt ccgatcgcgg tgcatgccca tgaaccgaat acggtgtatg
    12901 tggtgccgat caagagcgat tcagaacatt atccgccgga ggggaaactc cgggtgtatc
    12961 gcagcaagac cggcggccat gaatgggagg cactcacgaa gggtctgccg cagcaggatt
    13021 gttatgtgaa cattctgcgc gacgcgatgg cggtggatca gcttgagccc tgcggcctct
    13081 atttcgggac gaccggcgga caggtctatg cgtcccgcga cgggggtgac agttggacgg
    13141 cgattgtgcg ggatttgccg ggggtcttat ccgtagaggt gcaagcgctc taacgtctgc
    13201 tgaaaatggc cgccagcggc gttctcagtc gcgaccctca ctgcgacgta ccccgagggt
    13261 acgcctcatt cgcctcgctc cctgcggtct cgctggacga ccattttgag cagcgttatg
    13321 gagagagaag ccaggtacgg ctgttaaaca gccctgtgac tgccacgacg gttgagagtt
    13381 gagagaggat tgacggcccg tttccccgcg agaaacgaac gatggaggat acatgattcg
    13441 tgtcgtattg cccgcacatt tgcggacgtt ggcgcgtgtg acgggtgaag tggcgctgga
    13501 tgtggcaagt ccggtaacgc agcgggcggt gctcgatgcg cttgaggcgc ggtaccccat
    13561 gttgcgggga accattcgcg accatgtcac gcacaagcgg cggccgttca tccggttctt
    13621 tgcctgcgag caggatctgt cgcacgagtc ggctgatgtc cccctcccgg acacagtggc
    13681 atccgggacc gagccgttat tgatcgtcgg ggcgatggcg ggcgggtaac aggatggaga
    13741 gcatatggct aatagcctat ggcaaacggc gaaggcctga aggcacggct gaatcttgct
    13801 ctcctgtcat gggccatacg ccataagccg ttggctctta tcgaggagtg aactgtcatg
    13861 caaaagatag cgccatgttt gtggttcgac gatcaggccg aagaggcggc gaaattttat
    13921 gtggcgacgt tcagaaaggc caagctcggt cccatcacct actatggaga ggccggtgcg
    13981 acggtctcgg gaagaccgaa gggttcggtg atgaccgtgg cattcgaaat cgatgggcag
    14041 gaatttctgg ccttgaacgg cggtcccatc tttcaattta ctgaagcggt ttcgctgatg
    14101 gtgaagtgcg agacgcagga agagatcgat cacctgtggg aggccctgtc ccaaggcgga
    14161 gaaaagggac aatgcgggtg gctcaaagac aagtacggtc tctcgtggca gatcgtggtg
    14221 ccgcagtggg atgagatgtt gcgcgataag gatgccgcga ggtcggaacg ggtcatggcg
    14281 gagattctca gcatgaccaa gccggatctg cagtgcgttc aacgcgccta tgagggccga
    14341 tgacggcaag ccggcacagg agacgcaacc atgggtgtga agctgatctg gggtggacgg
    14401 gtcatgtccg gtctcctggc cggtttgctg acgatgagcg cagcgatgaa gttgatcggt
    14461 ggccggaagg tgatcgaagg cgttgcccgg atgggacttc ccgagtctct gcgcccccct
    14521 gagaacctag cgccctgtcg attttgggct ggtgcggtcg actaatagct agaaggcggc
    14581 cggtgcggga atgaggccgt cgcagaacca tgtcaccaat gaaggagtgt gtcccatgcg
    14641 atttatcgta tttgtcaaag ccaccaagaa ttcagaggcg ggtgtgatgc cgagccagga
    14701 gttgctgtcc gccatgatga aatataacga ggaactggta caggctggag tgatgcttgg
    14761 aggggagggg cttcacccga gttcgaaggg cgtacgcgtg aagttttcgg gtagcaagcg
    14821 gatcgtcacc gacggaccgt tcgttgagac gaacgagatc gtcgccggct attggttgtg
    14881 gcaatgcaaa tccaaagaag aggccatcga gtgggtcaaa cgctgtccca gtccgatgcc
    14941 aggcgaagaa tctgaaatcg agattcgtcc cttgttcgag gccgacgatt ttggagccga
    15001 gttgactcct gagcttagag agcgggaaga gcagctacgg gcgcaagcag ccggcaagca
    15061 gtaggacagt catggggggc ttttgcagca tggataagat ggtcgacaag gatttcgagg
    15121 atggcctggc ctccctcaag gcgttggccg ggaaataaca gtgccagggg tcttgtcaga
    15181 cttgatctga tcgtatgacg cgagcatagt tctagaagaa gagactcaac acatctcagt
    15241 cagactcagg aggccattat gcgatacatc acggtgacac tggttgcgct tgcgttggtc
    15301 atgacagcct cgtttggtga ggccaaagaa aagaagcatg acaaacaaat ggacccgcag
    15361 gcgatgatgg aactctggca gaagctggcc caacccggtg agccgcacaa ggcgtttgcc
    15421 ggcttagccg gcacctggac gacgacgacg aaggaatgga tggaacccgg gaaacctcct
    15481 acggaagcga gtgggacggc tgacatgaag atgctcttgg gcggacgttt tctctatcag
    15541 gagttcaaca gccagatgat ggggcagccg ttttccggaa tcgggatcga tgcctatgac
    15601 aacgtgcgca aaaaatatgt gactgcctgg atggacacca tggggaccgg catcttcatc
    15661 atggaaggaa cggcgagtgc ggacgggaag acgatcacgc tgaaggggtc tcatcccgaa
    15721 ccgggcggcg ggcagatgca tcatcgcgca gtctggaagt tggtggacag caatacgcag
    15781 acgttcgaga tgtacggaaa ccacgggcac ggaaaggaag aaaaaatgat ggagatcacc
    15841 tatacccgta agccataggt gcactctcct caaccctgtg acgtgacgtg tgagtatcga
    15901 gcggcgtgga ctgtgtggag gcatgaacac agtgccctgg gcgtggccgc catcagtggc
    15961 cggtgagtca tcaatttttc gaagggaggc cgttatgcaa cggacgatcg gattcctcgc
    16021 gatggcgctc attctgacaa gcttgagcgg ctgcagttat ctcttttacc cgcgagccgg
    16081 agactacgcc acgcaggcga aaggggcgaa cggtctcgag accatgacga acctcaccag
    16141 tatgatggag tcgaccgcct ccaaggcgaa gggtgggaag ggcgtcgaca tggccttcga
    16201 cgatctccac aatcagttcc atgccttgag tgattcgttc tgcggtgtga cggatgcgca
    16261 aagcaaaaca ccggcctatg cgttggcggt gacgcataag aaagaactta gtgcgatttt
    16321 caaacggctg tggaagttca aagatgatca gcctcagcga gaccaacacc ttgagttgtt
    16381 ggccactgag ttgaaagaac tccgtgagac gttgcggacg atcaagtagg gccagtcgta
    16441 gaggatgatg gcagcatggt ctgtcgggcg gaggctagtt gaatcacctt tgatacgtgc
    16501 tgcgcgctct ggcatggtca agtgaggaag atgttggaat ttacctagcc taggacgtca
    16561 gagttaagca tcttcagtca gggaacatga gtgggaagag ggtggcgaat ccaggcgtgg
    16621 gagactggga tctcgcgccc ccttcccatg atggcgtccc caccgaacca cgtgtcgtct
    16681 ccccgctcgg cgcaaccttc aagtctcgtg cagtccattc ttgaactaag ggaagagtac
    16741 tgtgccctga ttgcctacga gtgtctgcca cgcagatgag cgccgaggct ccgttgctta
    16801 cgcaggagcc aaccagtaag ataggcttcc agcaatatcc catgaactgc gtaatcagat
    16861 tttatcaggg agcgagcatg gccaggcgat cacgattttc aactgtagcc ccgatgatat
    16921 acgggtgcct ggcgttggcg ctgctctcaa gctgtaagca ggaagtcggt tcttcccagg
    16981 cccctccggt tccagaggtc ggcattgtcg tggcgacggc gcaggacgtg cccgatgagc
    17041 cggagttcat cggacagtcg gagtcatcgc ggccggtaga aatcagatcg caggtgacag
    17101 ggattctcaa agaatggttc tttaaggaag gccgtgacgt caagaaaggc gaccgcctct
    17161 atcagatcga cccggtgccc tttcatgccg caatgttgag cgcgaaggcg aaagtcgctc
    17221 aggcagaggc gcgactggtg caggccaagc agaatctagc ccgggtgaag cccttgttgg
    17281 ccgagcaggc cgtgagtaca aaagatgtcg atgatgcggt ggccgaggaa atggcggcga
    17341 aagcgaacct cgaaggcgcc aaggcggaac tggtcaaagc caagttcgat ctcgacaaca
    17401 cgctgatcgt ggcgcccatc agcggcatga tcgagcggac gcgtgtatat gaaggacgac
    17461 ttgtgtcggc ccagaccgat ctcctgacga tcattcagca ggtcgatccc atgtatgtga
    17521 tcgtcagcgc accggaaagt tttttattga agcggcagcg cgacagtacg gccaaacgga
    17581 ttcagcaccc cggcgtctat caattgcgcg gcgtcctgac ctttgcagac gggaccacgt
    17641 acgggcagga gggggtgttg gacctcctgg atgtcggact caaaaccgat acgggatcgc
    17701 ggcaggcccg ggtagtcttt cccaatcatg atcgcgtgct gctgcccggg cagttcgtgc
    17761 gggtccgctt caaggggacg gtcaagaccg gcgcgattct ggtgccgcag cgtgctgtgc
    17821 agcaaggcgc caagggctcc atcgtgtttg tcgtcgggaa tgaggacaag gttgagatgc
    17881 gggaggttca ggcgaccagc tggcaaggca accaatggat tgtcgagcaa gggttgcatg
    17941 tcggggaccg catcatcgtg gaaggtctgc ataagattgc gcccggcgcg tcggtgaagc
    18001 ctgtgccgct tcctgctcct gccgcgacga ccgttccgac tgccgccaag ccgcagccgg
    18061 agcgcgcctc gtgatctccc atttttttat cgaccgcccg atctttgcgt cggttctgtc
    18121 catcgtgatc atggtggtcg gcctggtagc gctgaaggcc ctgccggtcg ctcaatttcc
    18181 tgaaatcacc cctcccgtcg tccagatcga ggccgattac ccgggtgcca acgcggagat
    18241 cgtggccgac tccgtggcgc gtcccattga ggttcagctt ccgggcatcg ataatctgct
    18301 gtactacgac tccaccagca ccaacgacgg gcacatgtcg atcaagctga cgtttgagat
    18361 cggcactgac gtggacatcg cccaagtgca aacgcagaat cgggtgaaac tcgcggaacc
    18421 gcagctgccg cctgaggtcg tgcgccaggg gatcagcatc aataaggttt ctcccgattt
    18481 gctggcagtg gtggcgctaa gctccatcga tccgacgcat gacacggtgt atttgtcgaa
    18541 ctatgcgatt ctccgtgtgc tcgacaatgt gaaacggttg cgcggagtgg gcaatgcggt
    18601 ggtgttcggc tcccaaaact attcgatgcg gctgattctg gatcccatcc ggatggccca
    18661 gctcagcttg acgccgacgg acatcgccaa cgttgtgcgt gagcagaacc gggattttcc
    18721 ggccggaacg atcggccgtg agccggcgtt gaagggcacc gagctcacca ttcccgtcat
    18781 cacgcagggc cgattgacgg aggtgaagga gttcgaggag ctgatcgtcc gtgccatgcc
    18841 gaatggatcg atgatccacc tcaaggacgt agcccgcgtt gaactgggtg cacaatccta
    18901 tacgctggaa gggcgttgga acggcaaacc gaacgtgttt ttgctgacgt tcctgtcgcc
    18961 cggtgccaat gcgctcgaaa cggtgaagcg ggttcgggct gaactggccg aggtgtccaa
    19021 gagttttccc acgggcgtgt cctacgatat tccttacgac acgacacgct tcatcgatgt
    19081 ctccatcaaa gaagtggtga agacgctggc tgaagcgatg gtgctcgtca ttctggtggt
    19141 ctatctcttc ctccagagtt ggcgcgcgac cttgattccc ggcgtggcgg tgccggtctc
    19201 cctcgtcggc acctttgccg ggatgcaggc cttgggtttc tcgatcaata ccttgaccct
    19261 gttcggcatg gtgctggcga tcggtatcgt ggtggatgat gcgattgtcg tcgtggaaaa
    19321 ttgcgaacgg cacatgacgc aggggcggct ttctgcgaag aatgccgcca agcgggcgat
    19381 ggaagaggtg acggggccgg tcattgcgat tgtgttggtg ttgtgcgcgg tgtttgtgcc
    19441 ggtcggattc ttgggtggta ttaccggcga gttgtataag cagttcgcga ttacgatttc
    19501 gatcgcagtc attatctccg ggtttgtcgc gttgaccctc agtcccgcgc tctgtgctct
    19561 cgttctgaag cccggtgagg agcagcacca tggaattttt ggcctcttca atcgattctt
    19621 ttcgtggatg cagttgcggt atatctcgac ggtcggcctt gcgctgactc gacgtgtctt
    19681 gtcggtggcg gtgttcgcag tcctgttgat cggtgtgttc atgttgttca gggtcattcc
    19741 gagcagtttc ctgccggagg aagaccaggg ctacttcatc accatcgtgc agctgcccga
    19801 tggcgcctcc aagcagcgga cggatgcagt actgagcaag atcgagagct atttcctggc
    19861 cagcccggtc atccattcaa ccgatgccct gtccggtcag aacttcgtct tcaatacgcg
    19921 ggggccgaat gccgccacga tgttcgtgcc gttgaaacat tgggacgagc gctcggctcc
    19981 gcagcatcat gtaaaagcgc ttatcggcgc agcctatgga gagttcgcca agatcccgga
    20041 agcactcatt ctggctttca atgcgccttc tattcgggga cttggggcta cgggtgggtt
    20101 taccctgcag cttcaggatc cgagcagcgg ggatttcaat aagttctccg ccgccgctca
    20161 ggagttcgtc ggcaaggcgc ggcagaatcc ggcgatcggc gcgatcggca cgagttttcg
    20221 cgtcagtgcg ccgcgcattc ttgcgacggt gaatcgcgag cgggcgaagg cgctgggtgt
    20281 gccgatctca gaagtgttcg atacgatgca ggcgtatttc ggtaatttct acatcaacga
    20341 cttcatcaag ttcggtcgcg tcttccgggt gcagaccgag gccgatgcgc agtatcgatc
    20401 cacgccggag gatatttcga agatttatgt gcgggcgagt ggccctcagg gtaccaccat
    20461 gattccattg gacaccgtgg tgagcacgga gttcacgagc ggtcccgatc ctgtgacgca
    20521 cttcaatggc tacaataccg cgctggtgtt gggggcggca gcccctggat tcagttcagg
    20581 ccaggtgctg gatgcgctcg aacaagtggc gaaagaggtg ctggttccgc agggatacgg
    20641 gatcgattgg agcggcattt cctatcagga gcgcatggtc ggcagtcagt cgctttatgc
    20701 attcgcattc gggttgctga tggtgttcct ggtgctggct gcccagtatg aaagctgggt
    20761 ggtgccgttt gcggtgattc tggcggtgcc gatcggtctc ttcggcgcgc tcagcgcagt
    20821 ttggctcaag ggaatgacca acgatatcta cttccagatc gggctcgtga cactgatcgg
    20881 actctcggcg aaaaacgcga ttctcatcgt ggaatttgcg aacaaacggt tcgaggaagg
    20941 ccatccgttg ctggaagcga cgatcgaggc ggcgaagctc cggtttcgtc cgattgtcat
    21001 gacgtcgatg gcattcattc tcggcgtggt acctctggtc attgccacgg gcgccggcgc
    21061 tgccagccga aactccatcg gcaccggggt gttcggcggg atgctggccg ccacctttct
    21121 ggctatcttt ttcgtgccat tattctttgt gctgatccgc tcgttgagtc gccgcttgaa
    21181 aaaggagact cctccggcac aggatgcacc ggacaatccc gaaaaggagc gcgactatca
    21241 acatgcgtag actggccctg gttctttcct cgacccttct gacggcttgt gctgttggcc
    21301 cagactttac gagacctgac gcgacgaccc cggatgcctt ccgcatggcg gaggcgggtg
    21361 ggaatgctgc atcaatcgcg aatacaccct ggtgggagct gctcaaggat caagagctcc
    21421 aaaagctgat tcgtacggca ctggaagaaa ataaggacct ccagcgcgcg gcggcggcgg
    21481 tggaggagtt ccaagcccgc ctgtttatcg ctaaaacaga ttttgccccg cagatgaatg
    21541 tcacgtcgaa tgcgccgctg ttcggtcgga agaccaattt cttgtttccc ggcttcccca
    21601 atcccttcaa ctactacctc cagggaaatt tgtcctggga gatcgacatc tggggacgta
    21661 ttcgtcgatc caatgaggcc gcgcgtggcg atctgttgtc acgggaggag agtcggcgcg
    21721 cgattgtgtt gcagttggta agtggtgtcg cggaggccta ctttgagctg ttgcagttcg
    21781 atatgcaact cgacattgcg cggcgtacgc tcaagtcctg ggaggagtcc gtcaggatcg
    21841 ctcaggcgcg attgcggcag ggaatgattt ccaagctcga tgccgatcaa ttcgaggctg
    21901 aacgggctaa tgctgcggct aaggcggcgg agttcgaacg tcagaaggta cagaaggaaa
    21961 atcaactcag tgtgctgctg ggccggaatc caggccggat tgcccgcggg cattccttga
    22021 cggagcaagt gatgccgccg gacgtgcctc ccggccttcc ttctgaattg ttgcagcggc
    22081 gtcctgacat tctgcaggcg gagcaggatc ttgccgccgc cacggctcgc atcgggatgg
    22141 caaaagctga tcgctttccg aagctcagta tcaccggcat cctgggtgtg gcgagtccgc
    22201 atttgtcccg tttagtggcc aatgaaaccg cgttcggtgt cgcggggcca ggcttcgccg
    22261 gaccattgtt gaatgctcag atcctcggtt tccaacagaa agctgccgag gcgcaagcgc
    22321 gacaagccgt ggctcaatat gagcagtcgg tgttggtcgc gttcaaggag gtggaggatt
    22381 cgctggtcgc agtcagcacg gttcgtgaac agcgcacggc acaattgcag caggtcgacg
    22441 cgttgcggtc tgcgctgagt cttgccaatc ttcgttacaa gggtggcctg gcaaattacc
    22501 tggatgtgtt gattgcgcag cgcaatcttt ttgaggctga gttggcgctg atgggaaccc
    22561 atcggctaca tctcgtctcc attgtgcaat tatataaggc gcttggcggt ggctggaccc
    22621 cggaagggcg gccgccggtt caggccctgc cgctggtctc tacacaggaa acgcgatagt
    22681 gacgttgcct cttgaggcgt aggtctagtc tgttgctatg ccctgatact tgttcgctcg
    22741 cctgctttct cgtcgatcgc ccataagcta agccgcagcc ccttcacctc gcttcaacct
    22801 gagcgctccc ggatttttcg ggttgtgctt ccggtacctg aaagacattc acgttcgacg
    22861 atcggctgcc gatgtcaggg tgaggatcat ggcgaggctt ccacctaagg aggccatgag
    22921 caggatggtg aggccgctgt gtatccatgc tgagctggtt tggagtccca gtacgatatt
    22981 cgtaaggttg aaggtccaga gggttgccgc cacccatgcc aagtgttccg ctcggttggc
    23041 ggagaggcga gtgcccacca gggtgaagcc gagcactcct gacaacagat tacagagata
    23101 tgcggttgaa gaattgttct caagggttat tccggctgca gccatgcaca gccccgcggc
    23161 aaatgtcagc ccataaacga tcgcggcatc gcgtaccaca gttttggcgg tggaggtcac
    23221 tgcagcctcc cctgttgatg accgtctcct agccgactga cttctaattg cttcatgatc
    23281 aaaaagagaa gagtttgaga gatgccggta gaacgagtgc tcggtagtga cgcacatttc
    23341 atgctctcgg ctgggcgacg tttcacctgt gcctcctttt gaatctcggc ggaacgtgca
    23401 atcggaaacg aatgcagagg aggaacagac caacagtaac cggtctcctg ttccttgcgg
    23461 gtgcgaggtt gaatgacggg gcgagccgac atgctgtcat agcctttttt tctgggtcga
    23521 actgagaggc gggggagaag aaagtgattt tcggttgcgc agccaacggc tcaaacgtcg
    23581 agacatcgca tacaccaggt tatcaattgc gagtacgacc gcgccgatta aaaacagccc
    23641 cagaatgaga gcgagctcta catcaatgga cattgtttat tccaccggtc tagacatgag
    23701 tgagtagaga agagggatgc tcgttatctg catatatttc actgcaagac aatcgtgacc
    23761 aggatgcctc cggccgtgat ttccctgaca tcctggtctt tgcattgaga ctaggcagcc
    23821 agctgtgcca tgcctcctga tccgaagtgg gcatgctcac tgtttgggat cgtgggagcg
    23881 gatttggagg agtgcagtgc ggcgccctga gtctgtcggt tgtgctccaa caccttctcg
    23941 gccacatttc cgcagttcat gcatcgtttg gcttggagcc acatgggccc cgacgagtcc
    24001 tccatatcca gcaggaaatc ttcaaccatg catccgttgc agcgagaaca cgtcatagtg
    24061 gcctccttca ttgttcagtg cctatgtgta agcgtagaac cggatggtca tctccgacag
    24121 acctgaatga agtacctcct tggtggtacc gccaggtggg atgtttttac tggtggccaa
    24181 atattgatag gtgcatcgag tcttcgctcg ccgggaggcg cggagatgga agccactcgg
    24241 catcatgccc acctttttca agagaactat tcctgcc
//
LOCUS       DAORRQ010000058        24048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_122_length_24048_cov_59.610970, whole genome shotgun sequence.
ACCESSION   DAORRQ010000058 DAORRQ010000000
VERSION     DAORRQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24048
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_24048_cov_59.610970"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..124)
                     /locus_tag="PK224_15800"
                     /pseudo
     CDS             complement(1..124)
                     /locus_tag="PK224_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(236..535)
                     /locus_tag="PK224_15805"
                     /pseudo
     CDS             complement(236..535)
                     /locus_tag="PK224_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(846..1790)
                     /locus_tag="PK224_15810"
     CDS             complement(846..1790)
                     /locus_tag="PK224_15810"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRB17259.1"
                     /translation="MRAQPQFPNEQTDTGEFKRQSDAFRHWVTEDGRSGYPAAAGRYH
                     LYVSLACPWAHRTIIVRALKGLTEVIGMTVVDPIRDDQGWAFHNGPGHSLDTTNGFHF
                     LSEAYRATDPNYHGRVTVPVLWDRVTKRIVTNSDDDLMRIFNGEFNRFTTSRLDLYPA
                     ALRPQIDEMNDFVYERVNDGVYRAGFATSQQVYERAARSVFVALDQLDTRLRDRRYLF
                     GAQFVESDWRLFVTLLRFDAVYHGHFKCNLRRIVDYPHLYGYLRDLYQVKGIAETVDF
                     DHIKRHYYVTHDDINPTRIVPIGPQLDLESPHGRDTLS"
     gene            complement(1889..3916)
                     /locus_tag="PK224_15815"
     CDS             complement(1889..3916)
                     /locus_tag="PK224_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRB17260.1"
                     /translation="MFRLHHSTGVTCAFLLLIFGLPSVSHARPDKPQPKPNASLIHSD
                     RSIHRALTEGTAAQAKRLLDQGANIEARDAQGATPLITAAGRGNLALVTLLLNRHAEV
                     ETTDRAGNTALHQASFYGQVPCVEALLATGAQTSARNALAFTPLHQAVRRFWELSGES
                     RADRLTRQANVIDILLRYGADPELRDASGRTPITLATESSNGSLRHALNRTPVRAIPA
                     AEIPMRPQSSAATAESPQAAPGMAAPDLGTPSSAVQSNPPAPSRSAAPLPAVPSLNPP
                     TDMSAQDDRQPDIAVIPTPSTPATESSTGPTAAESLSPAPEGTSAATSPADATSRPHT
                     GPVPAPPIATGPTRDPEASKAIALQPLLTESQPPADTVTPLSPQVSDIPPTKSLTPQN
                     TIHPPTAPSPTPTPLIAAVDAQVETKPAASPSADQQRRTWTPVIESAEERRRPRQPPQ
                     LTPSTDSTRPHSAPLVASADSSSAAALAPQAGATGPSTDRTPLPTLAPATDKKENPPD
                     TDAERPWMFQRLGFGLGLGWTHNLGPRRVDSVTVVNRIVRIDNERNDLVRFMPEMHLW
                     IDRWDEQRWSWGPFLTVAPGSRIIDAVGFGLMMGYRPHRQDQYSFNVGIGGTLDLDAR
                     VLGDGLIANEPLPARETTARTKQTTAAGLLVLFSVGWDLAAPHHPAHTERQ"
     gene            complement(4214..4708)
                     /locus_tag="PK224_15820"
     CDS             complement(4214..4708)
                     /locus_tag="PK224_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17261.1"
                     /translation="MVRLTTDRMKSPRQWVTSLAVGFCTVLFTLPLHAADSSGPWECS
                     SYTGDAHTRCLQAFIEIQRDKISKLEAEVHVQQNAVGQLKEQADRQSAMTADLQRQMA
                     EQSSSVSTYNYVAPGLLYGYPSVGFGLYLGRPWLYGSPFYGRPYAWGPRYYRPYYGRW
                     HRRW"
     gene            4874..5824
                     /locus_tag="PK224_15825"
     CDS             4874..5824
                     /locus_tag="PK224_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HRB17262.1"
                     /translation="MGQKQVIAVVGATGMQGGGLVRAILADPSSGMTVRALTRDVNSD
                     KARELAQLGAEVVAADVHDGESLKRAFAGVAGVFCVTFFWAHCSPEKEFAEAEAMALA
                     AKSAGVPHVIWSTLEDTRRWVPLSDDRMPTLLGKYKVPHFDAKGEADQVFKQLGVPTT
                     FLLTSFYWDNLIHFGMGPKKGPDGTLAFTLPMGEARLPGIAADDIGKCALGLLKKRDA
                     YVGKTVGIAGEHLTGSQMAAALTKALGREVRYNAVPPEVYRTFGFPGADDLGNMFQFK
                     RDFNAMFCGAREPAIARALNPGLQTFAQWLEANKGRIPLS"
     gene            complement(5846..6076)
                     /locus_tag="PK224_15830"
     CDS             complement(5846..6076)
                     /locus_tag="PK224_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17263.1"
                     /translation="MRYSLYFAWVVAVLSICVLTTLASFPLLGSGERPAEPRIATGTE
                     KLRTIKLHRLRCNPFTPSCQLSKHKPSHKVPA"
     gene            6281..6556
                     /locus_tag="PK224_15835"
     CDS             6281..6556
                     /locus_tag="PK224_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17264.1"
                     /translation="MNRMSISTATIVLALFGLVSVGWAGEMKAKMEEMKGDTKAKVEE
                     MKGEAKATVEEAKGNKVQAEVERAKGKGNAAMEKAKGKVKELKAKTE"
     gene            6746..8209
                     /locus_tag="PK224_15840"
     CDS             6746..8209
                     /locus_tag="PK224_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRB17265.1"
                     /translation="MLLSFVILYLACSVGIGLYAATRVHNSKDFAVAGRCLPLPVVTA
                     TVFATWFGAETVLGISATFVKDGLRAVVADPFGSSLCLILAGLLFARRLYRLNLLTIG
                     DFYRLRYNRTVEVLCTLCIVASYLGWVSAQIKALGLVFNVVTDGAMSQSVGMVLGAVI
                     VLTYTTFGGMFSVAILDFVQITIIMGGMLYIGSVISGLAGGVERVVTHAAMAGKLDFF
                     PPARMEAWIPFLGAWVTMMFGSIPQQDVFQRITSARDERTAVRGSVLGGTLYFFFAFV
                     PMFLAYSATLVDPVQVAQLLERDSQLILPNLIVQHTPIVAQIIFFGALLSAIMSCSSA
                     TLLAPSVAFSENIVKGWVPTISDQGLLRVMRIVLVGFAATVLLFALNSEASIFKMVES
                     AYKVTLVAAFVPLLAGLYWPRATTQGAVLAITVGLGTWGGSELTVTLDQVWPPQLLGL
                     LAAAVGMVAGSLLPQWIEHPAGLSASGNRPSLSSPVE"
     gene            complement(8396..10756)
                     /locus_tag="PK224_15845"
     CDS             complement(8396..10756)
                     /locus_tag="PK224_15845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17266.1"
                     /translation="MDRPSHTDPAPDLHTLQVQVLYRNMPTGVVASAINAAVLAAVEW
                     SVVPHTPLLLWVVGLWMVAAARALLIVNYRRALPVSWSSADWHRWMLVTTSMAGFGWG
                     SSVYFLTLDIPLPYELVQLFVLGGMTAGAVSVLSVSFPLFLWYAVPLTLPILGHLFLS
                     GHEFHLIMGGMGVLFLLATIHSAWNFNRVLLSSMRLQLEKVALARSLTTRTEAVERLN
                     QDITKEIHDRRVIEEQLRTAQTDLEQRITERTADLAQANARLQNEVREHRRTEGARLS
                     SEKRFNYLTDNLNQGVWFAQANPPQVLYVNPAFERIWGLPAARFYENPKLWRDFIHPD
                     HQQVVSEVYDAEIANPTGRDLHLLYRIVRPDGQVRWIHDRMVIHHTATGEVDSLSGIT
                     EDITEARELEDQLRQAQKMEAVGRLAGGVAHDFNNVMTVILGYSAVLLQELSQNSSAR
                     YFVQEIQRAGERCAALTGQLLAFSRKQMLHPVSLDLHRVIRDLMALLKSLIGEHISIV
                     LQLDPLPLWVKADAVQLEQVLLNLAVNARDAMPHGGTLTIDTRQVSREAVWGSDHDTR
                     TARTYVRLRVHDTGTGIDPATKAKIFEPFFTTKPPGRGTGLGLSTVYGIVHQSGGTIT
                     VESAVGQGTTMTVFLPEVVPPLTALPPVQSEPAHTTGTEIILLVEDEPAVRLLTQHIL
                     RTHGYTVHEAEDGFQALDLIRRNALHIDLLLTDLVMPGMNGKELAMRLRSHFAELKVL
                     YMSGYSDNPPVTGDEAQGHTTFLQKPFSPEDLIRMVREILHSVSPA"
     gene            complement(10873..12309)
                     /locus_tag="PK224_15850"
     CDS             complement(10873..12309)
                     /locus_tag="PK224_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRB17267.1"
                     /translation="MPATRPSHHERTRRQRRWKRHDDAFPRLLIACCALIAMTAFLPP
                     AARAGSFRIYDHSASATGQASAFTAQADDASAGYYNPAGMTQLRGVQFSAGTTLIAGG
                     FSFQNGAGTQANGDLRGSVAVPPPSNVYITANLKDLGIGSSDSTTIGLAVFSPFGTLT
                     RWPDNSPFSTATTKAAFELIDIRPSIAFRPLPDLAIGLGADIYTFSGAFGEGQVERQF
                     RWPGGLGIPAGTGMELNGRDTAAGFNASLLYTPLRNGDGRPLVNIGLIYRSQATLHLD
                     GQLLAGGTRVADARTTFVVPQVLTGGIALWPVRDKEREWKLELDVDYTGWKSVRNLDT
                     TLSNGLTIATPANWTSGYTVMVGTEYKWLNPAPLPDWDVALRAGYWHSQKAIPDQTFN
                     PAIPDGDNHAVSTGFGFMCREHGKFLGVIPCGGSDKALSPKGLGIDLAYQAILYEGRT
                     VAGNVNPTVNGTYRTTLHVGSINLRVNF"
     gene            12636..14939
                     /locus_tag="PK224_15855"
     CDS             12636..14939
                     /locus_tag="PK224_15855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRB17268.1"
                     /translation="MVVIFVRRWIVRGLLVLAGVVVLAAGSAVAYGLYLSASLALPAG
                     DEHPPRLIYGAPFLLKPDLDIASAHLIERLNRLGYRSVETAVRTPGEYRVTPAEIDIY
                     LHEFADFHLRPVPARLALDQGRVTKVLSIPGGDELFPAYLEPQLISGLRGASRQVREW
                     VSYDDLPARFLDVLLAIEDRRFFSHPGIDPIAVGRAVWTNVTRGTVIQGGSTITQQLA
                     KNLFYSPQRTFGRKLKESIAALVLEAKYRKQAILESYANEIYLGQVGSVSIYGVGEAA
                     HRYFGKRVDALSLEETALLVGMIKGPNTYSPLRNPALAKQRRDVVLGRLHEQGLVSDD
                     AWKQAVMTPVRVMPPQDTLADAPFFVDHLLRQVEETTGAPLPDGVRAYTTLDPVLQRL
                     ATQTLESELGKLEAAYPALTGHSSPVQAALVALDPATGGILAMVGSRDYRTSQFNRAV
                     QSKRQPGSLFKPVVYLAALEARRELSGGQPITAATSIVDEPVTFESAAGVWAPQNYDH
                     RFHGTVSVRTAIEQSLNIPAVKIAQAVGTKRILQMAEKLGIRSPLADNLSIALGTSGV
                     SLLEITSAYGALAQGGLYVAPSALQAVMTPEGDPAWHRTPVRHQAVSPQAAYVMTSLL
                     RGVVERGTAAKAKAMGLRGTVAGKTGTTDGYRDAWFVGYTPDVVVGVWVGFDDEEALH
                     LTGAQAALPMWVEFVRRISPAATREFALPPAVVTRDIDPQSAQLATSKCPQRSAELFI
                     EGTEPSIYCEVHGSGFWERVKRSFGFS"
     gene            15032..15472
                     /locus_tag="PK224_15860"
     CDS             15032..15472
                     /locus_tag="PK224_15860"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRB17269.1"
                     /translation="MMKKSGFVENDNITLLAKGVLLRGEIRVEGTVRIDGRLEGDLHT
                     TGTVVIGEDGIVQGTITAGIIISSGKIKATVRATERLQLLKTGLLVGEVHAPAFSMED
                     GAKFQGMSDMGVASWGDEAQKLPGNVRDISSQRPKAVAMLGENA"
     gene            15647..16729
                     /locus_tag="PK224_15865"
     CDS             15647..16729
                     /locus_tag="PK224_15865"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRB17270.1"
                     /translation="MNRQQIFALCFFGVLLALLYQMGLMFRPFVFPVLWAVILAHLCF
                     PLHIRLTAWLGGRESHSAVLLTLAALALVVVPLVVLSVMLVKEAGSADRAVREWIASG
                     GVQQLPDRLSGLPGGPVVREWLERFTGRESDLEQFVLSSAKTFSRFIVGELSDLVRDV
                     FMLVADFLVMMFTLFFFFKDGRHWLASLYALIPLEASHKDKILARLDQTIRAVVKGIV
                     LTAIAQGLLAGAAYAVLGVPFPMVLMALTILLAPLPFGGTALIWGPVALYLLWAGTVG
                     KGLVMLAWGIGVVSTIDQILKPLFIGQGAEIPVLLLTFSVLGGLAVYGILGLFLGPIL
                     VGLFLTALQIYRDDYQQHLPAPPAAS"
     gene            complement(16753..17346)
                     /locus_tag="PK224_15870"
     CDS             complement(16753..17346)
                     /locus_tag="PK224_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB17271.1"
                     /translation="MRLILTVIRARPLWLALALACVSSYSLQAKDVAPPPGIPPEKVA
                     DFVHAVLDADRTIYTNQVVNRMQAKGIVSAAEHWEHENALPLPAQFLQHSGKLVAESG
                     RGIRYRLISLWPIYQRNAPATDLERRALESFSQTPDRPFTGIVTSGRKQYFQAIYSDR
                     AISAACVKCHNSHPLSPKRDFALNDVMGGMTITIPLD"
     gene            complement(17331..19811)
                     /locus_tag="PK224_15875"
     CDS             complement(17331..19811)
                     /locus_tag="PK224_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB17272.1"
                     /translation="MTSTSRSFSKALGWGLKRKLIVSMLMVGVVPLLLGLSLAFLQGS
                     KEIQVVSGESFQALATEAARKLDLLVAEEVARTSRIAVHPDIVRALEHRRDALHASDQ
                     NATLPTLVQQRARWAARDPAAIKAITGNSTALLLQGFYAGSQSEPDQLLPQVVRAATK
                     KLFITDVQGNLFAALTTKPGFSHADSPWWKGSFNRGVGQLYIEDLHFDEQANAYVFSI
                     SLPVMDSLRYEVVGVLHRVIDAKEFFSPSTHPIRFGKTGHVMLIDSRGMVLSCPILPT
                     GVQLSGASLIPQVTVRQPGWVTADSDGHGGQGTAIIGFAPLPETSRATNGSLENGAWH
                     TFVWQSSDELFAPIRHLMMWMMILAGIALGLLATLGYFAASRIVTPVRRLQEAARLIG
                     RGELQEPIQIHTGDELEELAVEFNRMNTQLEAAFAGLSTQVEEKTQEVQYLQKSTDQV
                     LDAVPTPIIMIDQQGFVQYMNRASRDAIQPQEQSWSGRLLFDLLPLDDDHLQALRRDL
                     RLVEENKPTAPSRQDQEPVLKEARDPLNQTLSQASPSARRELRIGAHLYHYEWFPLES
                     RSGAGKRFGLVLRDTTDESRLQDQLIQAEKSGSLDVLTAGIGHELNNPLFGILGLGEA
                     IQEEADLARAKGYAQDIVGHGRKMAAIIRDFTGVAARESKSQRVPVDVTAQLEQALAI
                     VQTSHDCLGLNVQKHYVAVSPVTALPDQLRLAFINVLTNAIQAMNGRGDLWLTTEEQD
                     DLITITIRDNGPGISKHHLGKVFDPFYTTKGQGEGSGLGLTVAQRLIKKFGGEIRLES
                     PEGQGTTCVITLPADKAGVRKEEPCVSS"
     gene            complement(19808..20857)
                     /locus_tag="PK224_15880"
     CDS             complement(19808..20857)
                     /locus_tag="PK224_15880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB17273.1"
                     /translation="MKLCATRIAIIGAGKGGTAILDLLHQIPDVEIIGIADKDPTAPG
                     LKRARDLNVLVAERVQDLIQNHGVNLIMDVTGDPSMEPLIHTMKRPGSEVLGGAATRL
                     LWKLVQHQSKLEAELFQADKLAGLGSFAAGIAHDINNPLQLILGLAENLEEEQDLAVV
                     HEQARDITEAVKRTSAICRDLTRYARRNGSREEVMVNLNTKLDEALRIARYAVTLQDV
                     TVVKRYAEEASVMGNPDELLHVFVNLITNAVQAIKDHGTLTLQTAVGPDGLVSASVSD
                     TGCGIPKEAFSKIFEPLYTTKPPGKGTGLGLYNVMSVISKMEGHICVESDVGVGTTFR
                     IEFPQVQPTISCAPL"
     gene            complement(20989..21423)
                     /locus_tag="PK224_15885"
     CDS             complement(20989..21423)
                     /locus_tag="PK224_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17274.1"
                     /translation="MGEFKSGFFMSESACNGRVLVVDDEPDIRKVVRMTLQKAGYEVI
                     EAENGEKAIEAINTGENRLLLDVLICDIRMPKINGVEAIAYFQQEWPRVPIIVLTGFP
                     DTDMATSFLRSGVVDYLVKPVEGEKLRTAVARAMEQRELAQL"
     gene            complement(21802..21978)
                     /locus_tag="PK224_15890"
     CDS             complement(21802..21978)
                     /locus_tag="PK224_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17275.1"
                     /translation="MRPTAAAGFGSEAIQGLGSYFLTLPYGEIKMAVAVLGTVAGGMG
                     VTFTDGEKVTSGKI"
     gene            22200..22496
                     /locus_tag="PK224_15895"
     CDS             22200..22496
                     /locus_tag="PK224_15895"
                     /inference="COORDINATES: protein motif:HMM:NF013851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRB17276.1"
                     /translation="MVVQIAFGFPYALFSRKMITAEAVTAFIRQVFPDAAVTVIDKTG
                     TQDHLIVRVTSGGFLGKNLLDRHRMVYQALAAPMKDGRIHALEITAKTPEEVNT"
     gene            22517..22843
                     /locus_tag="PK224_15900"
     CDS             22517..22843
                     /locus_tag="PK224_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRB17277.1"
                     /translation="MADPIEEEIQQEIKANKILIYGKGTKAMPMCGFTRETMHFFEKY
                     GYPYELIDVLSQPAKREALTKLTNWPTLPKVFIDGQFYGDTDILDPMEAKGEVVPLLK
                     KAFGAE"
     gene            23004..>24048
                     /locus_tag="PK224_15905"
     CDS             23004..>24048
                     /locus_tag="PK224_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRB17278.1"
                     /translation="MSEDFHPKSPLCVDLDGTLIRTDLLWESLLALLKQSPLSVFQLP
                     FWLLKGKASFKHEIARRVTLDASMLPYDQALVEFLSNERRAGRELVLATASHESFARA
                     VAAHLGLFDERVFGSDASTNLKGARKVALLVERYGARRFAYAGNSTADLPVWAEANEA
                     IIVNASAGLVSRAQTLTPVSRVFSEPVKWLKQVAKALRVHQWAKNVLVFIPVVASHQI
                     TNRALMLQATLAFLSFSFSASAVYIVNDCLDLASDRRHPRKKYRPFASGSLSIPFGLL
                     LAAGCLVGGILLALALPSPFLLVLAGYLVLTTGYSFYLKQFVLLDVIVLAQLYTVRVY
                     GGGAATGVAPSHWL"
BASE COUNT         4333 a         6751 c         7866 g         5098 t
ORIGIN      
        1 ctgcttcggt ccagcatttg atgacctggt tcagcgggaa ggtggccggt tcgccgcgtt
       61 tgaccgagct gtcgaacacc gttccgtcga tcagcgtccc gtggtagtgg actttcaccg
      121 tatcggtggc cttcggggtc gcgcccttgc cttccttgat cgtcgtgacg atcgcacccg
      181 attccgtttt cttcgcgccc gactccgccg cagccttggc caggaatcct gctccggctt
      241 tcttttcgac ctcggccagc gccgtggcgc gtacctgctg catctgttga atcttcggac
      301 cgtacacttg caaatcgacc ttttggggat gtttcaagac accatcgacc aaaccggctt
      361 tcacgaattc caattcggat tcgttcaaag taaacgggcc gagcgattgg ctgatggccg
      421 caccgagggc atagagcgtt ttctgctcgt cgttggccgg ctcaggggcg gccgcccagc
      481 tgagcgagga tatgaggagc accactgcgg agacggcacc ggtcacatga cgcatagacg
      541 atcctttctg atgaacaaca tgactctgct caccatatgc cagggagccc ttgcggctca
      601 agaaaattct tgggcaggct gctgaaaacg gcctcccgcg tcgttctcgg ctcgtcaaaa
      661 tcctcgacgt acccctgcgg gtacgcctcc ggttttgact cgcctgcggc ctagcgggac
      721 gtcgttttga gcagcctgcg aagattacgc agacttgtct gtagccttgc cgaatggaag
      781 gcctgtctgg cgtccaggat gggtaagtat gtcgtctgtt tcagcagtct tgccgtgagg
      841 tgcggtcagg agagcgtatc acgtccgtgc gggctctcca gatccagttg tgggccgatg
      901 gggacgatgc gggttgggtt gatgtcgtca tgggtgacgt agtagtgccg cttgatgtga
      961 tcgaaatcca cggtctccgc gatacccttc acctggtaga ggtcccgaag gtagccgtag
     1021 agatgggggt agtcgacgat gcgccggagg ttgcatttga aatgcccgtg gtagacggca
     1081 tcgaaccgga gcagcgtgac aaacaaccgc caatcggact cgacgaattg ggcaccgaag
     1141 aggtagcgcc ggtcgcggag cctcgtgtcg agttgatcca gcgcaacgaa gaccgagcgc
     1201 gcggcgcgtt catacacctg ttgggaggtc gcaaaaccgg cgcggtagac cccgtcgttc
     1261 acgcgctcgt atacaaagtc gttcatctcg tcgatctgcg gacggagagc tgccgggtac
     1321 aggtccaacc ggctggtggt gaagcgattg aactcaccgt tgaagattcg catgaggtca
     1381 tcgtcggaat tggtcacgat tcgttttgtg accctgtccc acaacaccgg aaccgtcacg
     1441 cggccgtggt aattggggtc ggtggcacga taggcctcgc tgaggaagtg aaacccattg
     1501 gtcgtatcca gggagtgacc ggggccgttg tgaaaggccc atccttggtc gtcccggatc
     1561 gggtcgacca cggtcatgcc gatcacctcg gtgagccctt tgagcgcgcg gacgatgatg
     1621 gtgcgatgcg cccaggggca ggcgagggag acgtagagat gataccgccc ggctgcggcc
     1681 ggatatccgg agcggccgtc ctcggtgacc cagtggcgaa acgcgtcgga ctgccgtttg
     1741 aattcgcccg tgtcggtctg ttcattcgga aactggggtt gtgccctcat tgcacgattc
     1801 tccttgggag ttctacctta gtcagaatca agggggctcg tcaattgtcg atagggccgc
     1861 gcgatgtcgt ggtgcctcga atgtaaggtc attgtcgttc ggtgtgtgct gggtggtggg
     1921 gcgctgcgag gtcccatccg acggaaaaga gtacgaggag accggcggcg gtggtctgct
     1981 tcgtgcgggc cgtggtttcc cgagccggca ggggctcgtt cgcgatgagg ccgtcgccca
     2041 ggactcgggc atcgagatcg agcgtgccgc cgataccgac attgaaactg tattggtcct
     2101 gtcgatgcgg tcggtatccc atcatcaggc cgaaccccac ggcgtcgatg atccgcgacc
     2161 ccggcgcgac cgtgagaaac ggcccccaac tccaacgttg ctcgtcccac cggtcgatcc
     2221 agaggtgcat ttcaggcatg aagcgcacca aatcgtttcg ttcattgtcg atgcggacga
     2281 tacggttgac gactgtgacg gaatccaccc gtctcgggcc aaggttatgt gtccatccga
     2341 ggccgagccc gaagccgagg cgctgaaaca tccatggacg ttcagcgtcc gtgtccggcg
     2401 gattctcctt cttatccgtg gcgggagcca gggtcggcag cggtgtgcgg tcagtcgagg
     2461 gacctgtcgc gcctgcctgc ggagcaagag ctgccgcaga ggaggagtcg gctgaagcga
     2521 caagcggagc ggagtgaggg cgcgtcgaat cagtggatgg agtgagctgg ggcggttgcc
     2581 gcggtcttcg tctttcctcc gcagactcga tcactggtgt ccaggtacga cgctgttgat
     2641 ccgccgacgg tgaagccgca ggtttggtct ccacctgggc gtcgacggcg gcgatcagtg
     2701 gtgtcggcgt tggtgaaggc gcagttggag gatgtatcgt gttctgcggt gtgagcgact
     2761 tggtcggcgg tatatcggat acctgcgggg acaacggtgt caccgtgtca gcgggtggtt
     2821 gggattcagt gagcagaggt tgcaaggcga ttgccttact ggcttcggga tctcttgtgg
     2881 gaccggtcgc gatcggcggc gccggtaccg gacctgtgtg cggcctggaa gtggcatcag
     2941 cgggcgaagt ggccgccgaa gtcccctcag gtgcgggaga gagggattcc gcagcggtcg
     3001 gaccggtgga ggattccgtc gctggagtgg atggggtggg gatgactgcg atatccggtt
     3061 gcctgtcgtc ctgcgccgac atgtctgtcg gcggattgag cgacggtacg gccggaagcg
     3121 gggccgcgct tcgagacggc gccggagggt tactctggac cgcgcttgag ggtgtcccca
     3181 gatccggagc cgccataccg ggagctgcct gtggactttc tgccgtcgcg gcggacgatt
     3241 ggggcctcat cggtatctcg gcggctggta tggcccgcac cggtgttctg ttaagtgcgt
     3301 gccggagtga accattgcta ctttcggttg ccagagtgat cggagttctt ccgctcgcat
     3361 ctctgagctc aggatcggct ccatagcgaa ggagtatgtc gatcacgttg gcttgccggg
     3421 tcagccggtc cgcccgggat tcaccggaga gttcccaaaa tcgcctgacg gcctgatgga
     3481 gcggcgtgaa tgcgagggcg ttccgtgccg aggtctgcgc ccctgtggcc aggagtgcct
     3541 ccacgcaggg tacctgccca taaaaactcg cctgatgtaa cgcggtattg ccggctcgat
     3601 ccgttgtctc aacctccgca tgccggttga ggagtagagt gaccagcgcc agattgcccc
     3661 tgcctgcggc tgtgatcagt ggcgtcgcgc cctgggcgtc tcgtgcctcg atattcgctc
     3721 cctggtcaag cagccgtttg gcttgggccg cggttccttc ggtgagcgcc ctgtggatcg
     3781 agcggtcgga atggatgagc gaggcgttcg gtttgggttg cggcttatcg ggacgcgcgt
     3841 gactgacgga cgggagtccg aaaatgagaa gcaggaaggc gcaggtaacg ccggtgctgt
     3901 ggtgcaagcg aaacacgggt ccccaacaag gcaattcaag aggttctcgg ccggtgtatg
     3961 gtttagcata cagtccgcat tggcgtcacc tgagtgtgcg accggggtgg aacaatgtga
     4021 ttagcgaagc cgccgctcgg tcttgcatgg gccgttgggt gggcaatgaa ttgatggtcg
     4081 ggtcagtctt caggaagagg tgggagcggc agagaaggga taacgcggtg aggccttgcc
     4141 aggggcggac cctccggttg gaagagcact gagaggatca cgtttcagca gctgtggcgc
     4201 ctatggtccg ggattaccaa cgtctatgcc agcgtccata gtagggccga tagtaccgtg
     4261 gtccccaggc gtagggccgg ccatagaacg gtgagccata gagccatgga cgtccgaggt
     4321 acaggccaaa acccacagac ggatatccgt agagcaatcc gggcgcaaca taattgtagg
     4381 tggataccga agaggattgc tcggccatct gacgttgaag gtcggcggtc atggcacttt
     4441 gccgatcagc ctgctctttc agctggccga cggcgttttg ctggacatgg acctctgctt
     4501 ccagcttcga aattttgtcg cgctggatct cgataaacgc ctgcaggcat cgcgtgtgcg
     4561 catcacccgt gtagcttgaa cattcccagg ggccggaaga gtcggcggca tgaaggggaa
     4621 gcgtgaagag gacggtgcag aaaccgactg cgagagacgt gacccattgc cgaggagatt
     4681 tcattcgatc agtcgtgaga cgtaccatga cgttcctccc tggctagcta gctacagcct
     4741 accattcccc tggcgatgaa gaccaggtct ggggctaacc ttccgaacga accacgcgct
     4801 gatcggttga caactggctg cagggaaagg atgcttggcg gtggcaaccg gttgtggaaa
     4861 ggagagggga gaaatgggac agaagcaggt gattgcggtg gtcggggcga cgggtatgca
     4921 ggggggcggg ttggtgcggg cgattctggc cgatccaagc agcggcatga cggtgcgcgc
     4981 gttgaccagg gatgtgaatt cggacaaggc cagggagctg gcgcaacttg gcgcagaggt
     5041 ggtcgcggcc gacgtgcacg atggcgagag tttgaagcgt gcgtttgcgg gcgttgccgg
     5101 ggtgttttgc gtgacctttt tctgggcgca ctgttctccc gaaaaggaat ttgccgaagc
     5161 cgaggcaatg gccctggccg ccaagtcggc aggggttccg catgtgatct ggtcgacact
     5221 cgaagacacg cgtcgatggg ttccgctctc ggacgaccgg atgcccacac tattgggcaa
     5281 gtataaggtg ccgcatttcg acgccaaggg cgaagccgat caggtgttca agcagctcgg
     5341 cgtgccgacg acttttctgc tcacctcgtt ttattgggat aacctcatcc atttcggtat
     5401 ggggccgaag aagggaccgg acggtacgtt ggcgttcacg cttcccatgg gcgaggcgag
     5461 attgccgggt atcgcggcgg acgatatcgg gaagtgcgcc ttgggattgc tgaaaaagcg
     5521 ggatgcctat gtgggaaaga ccgtcggaat cgcgggggag catctcaccg gatcacaaat
     5581 ggccgcggcg ttgacgaagg cgctgggtcg ggaggtgcgc tataacgcgg tgccgccgga
     5641 ggtctaccgg acgttcgggt tccccggagc ggatgatctg ggtaacatgt ttcagttcaa
     5701 gcgtgacttc aatgcgatgt tttgcggcgc gcgcgagccg gcgatcgccc gggcgctgaa
     5761 tcccgggctg cagacctttg cgcagtggtt ggaggcgaac aaggggcgaa ttcccctgtc
     5821 gtagacggag ccaggttcct aactgctaag ccggcacttt gtgtgacggc ttgtgcttgg
     5881 aaagctggca cgaaggtgtg aagggattac atcgcagccg gtggagcttg atcgtccgga
     5941 gcttctctgt ccctgtcgca atccgcggct cggcaggccg ctcgccgctc ccgagtaacg
     6001 gaaacgaggc gagcgtcgtc aggacgcaga tcgaaaggac ggccaccacc catgcgaagt
     6061 acaatgagta acgcatggcc tattcgtacc atagcgacat gagatgtcaa cgaaacgatt
     6121 gttttttgcc gtctgttcct gtcgctgagg atggagggat ctgttccgga agattccgta
     6181 gtcggtttca ggaattgctc cggcgttgtc attggcagac actgctgtga tgggtatcgg
     6241 attccgctgt cactcaccag tctcacaagg aggactcccc atgaacagga tgtcgatttc
     6301 caccgcgacc atcgtattgg ctctgttcgg cctcgtgtct gtcggctggg ccggtgagat
     6361 gaaggccaag atggaggaga tgaagggcga cacaaaggcg aaggtcgaag agatgaaggg
     6421 ggaggccaag gccaccgtcg aagaggccaa ggggaataag gtccaggcgg aagtggagcg
     6481 cgccaagggc aaggggaacg cggccatgga gaaagccaag ggcaaggtca aggaactgaa
     6541 agcgaagacg gaataggttc gatcttccga cgcggcgcga ccggcctgtg catggggatg
     6601 acgcatgcgc aggccggccc gtaaccgacc gcgtgagcgc ctgtcgtgct ccacctgcca
     6661 ttgcgcaggc gcttcgctgc cggtacaatc cccctcgctc gtcacccgcc gatgggtgtg
     6721 gtctcaccga cgcgcgaatg actccatgct cctatccttc gtgattctct atcttgcctg
     6781 ctcggtcggg atcgggctct acgcggcgac ccgcgtgcat aactccaaag acttcgccgt
     6841 cgccgggcgc tgtctgccgc tgccggtggt gaccgccacg gtgtttgcga cctggttcgg
     6901 cgccgagact gtgctcggca tctccgccac attcgtgaag gacggactgc gagccgtcgt
     6961 ggccgatccc ttcggatcga gcctctgttt gatccttgcc ggattgttgt tcgcccgtcg
     7021 gctctatcga ctgaaccttc tcaccatcgg agacttttat cggctgcggt acaatcgcac
     7081 ggtcgaagtc ctctgcacgc tctgcatcgt cgcttcgtac ctcggttggg tttcggccca
     7141 gatcaaggcc ctcgggctgg tcttcaacgt cgtgacggat ggagcgatga gccagtcggt
     7201 cggcatggtg ctgggagcgg tcattgtcct gacctacacc acgttcgggg gcatgttctc
     7261 ggtggcgatt ctggattttg tgcagatcac catcatcatg ggcggcatgc tctacatcgg
     7321 ctcggtgatc agcggattag ccggcggcgt ggagcgggtg gtcacgcatg cggccatggc
     7381 gggcaaactg gattttttcc cgccggcgcg gatggaggcg tggattccgt ttctcggcgc
     7441 ctgggtcacg atgatgttcg gatcgattcc gcagcaggat gtgtttcagc gcatcacatc
     7501 ggcgcgggac gaacggacgg cggtgcgagg atcggtgctg gggggaacgc tctatttctt
     7561 cttcgcgttt gtgccgatgt tcctcgccta ttcggccaca ttggtcgatc cggtgcaggt
     7621 ggcgcagtta ttggaacggg attcccaact catccttccc aacctgatcg tgcagcatac
     7681 tccgatcgtg gcgcagatca tctttttcgg cgcgctactg tccgccatca tgagttgctc
     7741 gtccgccacg ctgctcgcgc cgtcggtggc gttcagtgaa aatatcgtga agggctgggt
     7801 gcccacgatc agcgatcaag ggctgttgcg ggtgatgcga atcgtcctcg tggggtttgc
     7861 ggccacggtc ctgctgtttg cgctcaattc agaggccagt atttttaaga tggtcgagag
     7921 tgcgtacaag gtcacgctgg tggcggcctt cgtaccgttg ctggccggat tgtactggcc
     7981 cagggccacg acacaagggg cggtgctggc gatcactgtg gggctcggaa cctggggtgg
     8041 atcggagctg accgtcacgc tcgatcaggt ctggccgccg cagcttctgg gcctgctggc
     8101 cgccgccgta gggatggtgg ccgggtcctt gctgccgcaa tggatcgagc atccggctgg
     8161 attgagcgcg tccgggaacc ggccgtccct ttcctcgccc gtcgagtgag ctaggagcct
     8221 gtctgacatt gaccgttcga ctgcggcaaa cgatccacga tcatttgcgt cgttctcggc
     8281 ctgaaaaaat cctcaacgta tttcagcgaa tacgcttccg gtttttccag gcctgcggcc
     8341 tcgcatctgg tcgcgccttc ttcgcctcgc tacgaaggcc atgtcggaca ggctcctagg
     8401 cgggcgagac tgagtgaaga atctcgcgca ccatgcggat caaatcctcg ggcgagaagg
     8461 gcttttgcaa aaatgtcgtg tggccctgag cttcatctcc tgtgacaggc gggttatcgc
     8521 tgtacccgga catgtagagc actttcagct ccgcgaagtg actgcggagg cgcatggcca
     8581 actctttgcc gttcatgccg ggcatcacga gatccgtcag cagcaggtcg atgtgcagcg
     8641 cgtttcgtcg gattagatcc agggcctgga agccgtcctc cgcttcgtgc accgtgtagc
     8701 catgtgtgcg aagaatatgc tgcgtcagca ggcgcaccgc cggctcgtct tccaccagca
     8761 ggatgatttc tgtccccgtc gtgtgggccg gttcagattg aaccggcggg agggccgtca
     8821 gtggggggac gacttccggc agaaacacgg tcatggtggt gccctggccc accgcgctct
     8881 cgaccgtaat ggtcccgccg ctttggtgca cgatgccata caccgtggag aggccgagac
     8941 cggttccgcg gcccggcggt ttggtcgtga aaaagggctc gaaaatcttg gccttcgtgg
     9001 ccgggtcgat gccggttccc gtatcgtgca cccgcagacg cacataggtg cgggcggtgc
     9061 gcgtgtcatg atccgatccc cagaccgctt cccgggaaac ctggcgcgta tcgatggtca
     9121 gcgtgcctcc atgcggcatg gcgtcgcggg cattcacggc caggttgagc aggacctgtt
     9181 cgagttggac ggcgtcggct ttgacccaga gcggaagagg gtcgagttgc aggacgatcg
     9241 agatgtgttc accgatcagg ctcttaagca gcgccatgag gtcacgaatc acccggtgaa
     9301 ggtccagtga gaccgggtgg agcatttgtt tcctgctgaa ggccagtaac tggccggtca
     9361 gtgccgcaca gcgttccccc gcgcgttgaa tttcctgaac aaagtagcgg gccgaagagt
     9421 tctggctcag ttcctggagc agcaccgcgc tatagccgag gatgacggtc atcacattgt
     9481 tgaagtcgtg cgccacgcct cccgccaatc gcccgacggc ctccattttt tgagcctgtc
     9541 gcaactggtc ttccagttcg cgggcttccg taatgtcttc agtgatcccg ctcagactat
     9601 cgacttcccc cgtcgcggta tggtggatga ccatccggtc gtgaatccat cggacctgcc
     9661 cgtccggacg tacgatccga tacagcaggt gcaggtcgcg tccggtggga ttcgcaatct
     9721 ccgcatcgta gacctcgctg acgacctgtt ggtggtccgg gtggataaag tcccgccaga
     9781 gcttcggatt ttcatagaat cgcgcggcag gcaatcccca gatccgctcg aaggccggat
     9841 tgacgtacag gacctgcggc ggattcgcct gcgcaaacca cacgccctgg ttgagattgt
     9901 cggtgagata gttgaatcgc ttttcgctcg aaagtcgcgc gccttccgtc cgccggtgtt
     9961 cccggacttc attctgcagc cgcgcgttgg cttgggccag gtcggccgtg cgttcggtga
    10021 tgcgctgttc cagatcggtc tgcgcggtgc gtagttgctc ttcgatgacg cgccggtcgt
    10081 ggatctcttt ggtgatgtcc tgattcagcc gttccaccgc ttcggtgcgg gtcgtcagcg
    10141 accgtgccag ggccaccttc tccagttgca ggcgcatcga ggagagcagc acgcggttga
    10201 agttccaggc cgaatggatg gtggccagga gaaacaggac gcccatgcct cccatgatca
    10261 gatggaactc gtgtccgctg agaaagaggt gtccgaggat tggaagtgtc aacggaaccg
    10321 cgtaccacag aaacagggga aacgagacag agagcacgga caccgcgccg gccgtcatgc
    10381 cgccgagcac gaacagttgg accaattcat aggggagggg aatgtccagc gtcagaaaat
    10441 acacgctgct gccccatccg aatcccgcca tgctggtggt caccagcatc cagcggtgcc
    10501 agtctgcgct gctccaggag accggcagcg ctcgccgata attgacgatc aggagtgccc
    10561 gcgcggcggc gaccatccac agaccgacga cccagaggag caggggcgta tgcggaacga
    10621 cggaccattc caccgccgcg agaacggcgg cattgatggc gctcgccacc acgccggtag
    10681 gcatattgcg gtacaagacc tgaacttgta gggtgtgcag atccggggcc ggatcggtgt
    10741 gggatgggcg atccatacga aggcctcgct ctgaagcagg ttcaccgcgc acagagcacc
    10801 gatggcagca tcacgatgtg cgcgtgtctc gtaagtaagc ctactctacc atgatcgttg
    10861 gaaggccggg catcaaaaat tcacacggag attgatggag cccacatgca aggtcgtgcg
    10921 atacgttccg ttgacggtgg gattcacgtt cccggcgacg gttctgcctt cgtacagaat
    10981 ggcctggtag gccagatcga ttccgaggcc tttggggctg agggctttgt cggatcctcc
    11041 gcaggggatg accccgagaa acttgccgtg ttcccggcac atgaacccga agccggtgga
    11101 gacggcatgg ttgtcgccgt cgggaatggc aggattgaag gtctgatcgg gaatcgcctt
    11161 ctgggaatgc cagtagccgg cgcgtagggc gacgtcccaa tcgggcagcg gtgcggggtt
    11221 gagccacttg tattcggtgc cgaccatgac ggtgtagccg ctggtccagt tggcgggggt
    11281 ggcgatggtg agaccgttcg acagggtcgt atccagattg cggactgatt tccagccggt
    11341 gtaatccacg tccaattcca gcttccattc ccgctcttta tcccggacgg gccagagcgc
    11401 gatgccgccg gtgagaacct gcggaaccac aaaggtcgtg cgggcgtctg ccacacgcgt
    11461 gccgccggcg agcaattgcc cgtcgagatg cagcgtcgcc tgactgcggt agatgaggcc
    11521 gatgttgacg agcggacgcc cgtccccatt acgcagcggc gtgtagagca ggctggcatt
    11581 gaagcccgcg gctgtgtcgc gcccatttaa ttccatgccg gtgccggccg ggatgccgag
    11641 cccgccgggc cagcgaaatt gtcgctcaac ctgcccttct ccgaaggctc cggaaaaggt
    11701 gtagatatcc gctcccaacc cgattgccag atccggcagc ggccggaagg cgatggaggg
    11761 gcgaatgtcg atcaattcga acgcggcttt cgtggtcgcc gtggaaaagg gactgttgtc
    11821 cggccagcgc gtcaaggtgc cgaacgggct gaagacggcc aagccgatag tggtgctgtc
    11881 ggaggagccg atgccgaggt ccttgagatt ggccgtaatg tagacgttcg acggaggcgg
    11941 aaccgccaca ctcccgcgaa gatcgccgtt ggcctgggtg cccgcgccat tctgaaacga
    12001 gaagccgccg gcgataagag tggtgccggc tgagaactga accccgcgga gttgtgtcat
    12061 gccggccgga ttatagtacc cggcggaagc atcgtcggct tgcgccgtga atgccgacgc
    12121 ctggccggtt gccgaggcac tgtgatcgta gatgcgaaaa cttcccgccc gggctgccgg
    12181 tgggaggaac gcggtcatgg caatcagcgc gcaacaggcg atgagcagcc gggggaaggc
    12241 gtcatcgtgt cgcttccacc ggcgctgccg tcgggttctc tcgtggtgcg acggccgtgt
    12301 cgcgggcacg cagggttcct gtgaatcacc ggcatcaaga aagcgtagag tactcacggt
    12361 catattcctc gtgtggatga gtctgtcagt aacgcaatca gcctgtccgg gcgcgtggcg
    12421 tttgcgagta acgttgtacg ggtgtgacgc gcagagccga cgcgtatttc ggacgtcccg
    12481 atgggtgcaa gggcgtagaa attcggcaat gtggcgctca tctaagagaa ggcacggtgc
    12541 tttgtcaagc ttgggtgccc cggaggatct tcctaaagcg gtgtgcacgc atgtgatttc
    12601 gttcgctgga tcgcgtatgc tcggcagatg gggacgtggt ggtgatattc gtcagacgct
    12661 ggatcgtgcg ggggcttctg gtattggccg gcgtcgtggt ccttgccgca ggaagtgcgg
    12721 tggcctacgg gctctacctc tcggccagtc tggcgttgcc ggccggcgac gagcatcccc
    12781 cccgcctgat ctacggcgcc ccgtttctgt tgaagcccga tctggatatt gcctccgctc
    12841 atctgatcga gcgcctcaac cggctcggtt atcgctcggt cgagacggcc gtgcgcaccc
    12901 ccggcgagta tcgtgtcact cctgcagaga tcgatattta cttgcacgag tttgccgatt
    12961 ttcacctgcg accggttccg gcccgtttgg cgctcgacca ggggcgagtg acgaaggtcc
    13021 tgtcgattcc ggggggcgac gaattattcc ccgcctatct ggaaccgcaa ttgatcagcg
    13081 ggttgcgcgg cgcctcgcgg caggtgcggg aatgggtgtc gtacgacgat ctgccggcgc
    13141 ggtttctcga tgtgttgctg gcgatcgaag accggcgatt cttcagccat cccggcatcg
    13201 atcccattgc cgtgggccgc gcggtctgga cgaatgtgac ccggggcacg gtcatccagg
    13261 gcggcagtac gatcacgcag cagttggcga agaacctgtt ttattctccg caacgcacgt
    13321 tcgggcggaa gctcaaggag tcgattgccg cgttggtcct ggaggcgaaa taccgcaaac
    13381 aggcgatcct cgaaagttat gcgaacgaga tttatctcgg gcaggtcggg tctgtgtcga
    13441 tttatggagt cggggaggcc gcgcaccggt atttcggcaa acgcgtggat gcattgagct
    13501 tggaggagac cgcgctgctg gtcgggatga tcaaggggcc gaatacctat tcacccctga
    13561 ggaatccggc gctggccaag cagcgtcgcg atgtggtgct gggccggttg cacgaacagg
    13621 gactcgtgag cgacgacgcg tggaagcagg cggtgatgac gccggtcagg gtgatgccgc
    13681 cgcaggacac attggccgat gcgccgtttt ttgtcgacca tctgttgaga caggtggaag
    13741 agacgaccgg ggcgcccttg ccggacggcg tcagggccta cacgacgctc gatccagtgt
    13801 tgcaacggct ggccactcag acgctggaga gtgaactggg caaactggaa gcggcctatc
    13861 cggcgctgac cggccacagc agtccggtgc aagccgcgct ggtggcgctc gatcccgcaa
    13921 cgggcggcat tcttgcgatg gtgggcagtc gtgactatcg caccagtcag ttcaaccggg
    13981 ccgtgcagtc gaaacgccag cccggctcgt tgtttaagcc ggtggtgtat ctcgcggcgt
    14041 tggaggctcg ccgtgaatta agcggtgggc aaccgattac tgcagccacc tcgatcgtcg
    14101 atgaaccggt gacgtttgag tcggcagccg gggtgtgggc accgcagaat tacgaccatc
    14161 ggttccacgg cacggtgtcc gtgcggacgg cgatcgaaca atcgctgaat attcccgccg
    14221 tgaagatcgc acaggccgtg gggacgaagc gaattctcca gatggcagag aagctgggga
    14281 ttcgcagccc gctggccgat aatctgtcca ttgccttggg gacttccggc gtctcgctgt
    14341 tggagatcac ctcggcctac ggagccctgg cacaaggggg gctctatgtg gctccctcgg
    14401 cgttgcaggc cgtgatgact ccggaagggg atcccgcctg gcatcgtacg ccagtgcggc
    14461 atcaagcggt gtccccgcaa gcggcctatg tcatgacctc gttgctcagg ggtgtcgtcg
    14521 agcgcggcac ggcggccaag gcgaaggcga tgggcctgcg cgggactgtg gcgggaaaaa
    14581 ccgggacgac ggatgggtat cgcgacgcct ggttcgtcgg gtacacgccg gatgtggtgg
    14641 tcggtgtgtg ggtgggattc gacgacgagg aggcgctgca tctgaccgga gcacaggcgg
    14701 cgctaccgat gtgggtggag ttcgtgcgcc ggatctcgcc ggctgcgacg cgcgagtttg
    14761 cgttgccgcc tgccgtggtc acgcgcgaca tcgatcccca gtcggcccag ttggcgactt
    14821 cgaagtgtcc gcagcggtcg gcggagttgt ttatcgaagg tacggagcct tcgatctact
    14881 gcgaagtaca cggcagcggc ttctgggaac gggtcaagcg cagttttggt ttttcctagg
    14941 tacgatgcac aagctggtgt aggctcggct catgttggga tggtcctcca gttcaagttc
    15001 atcggacatt gcctgggaga aggagcgcgt gatgatgaag aagtccggct ttgtggaaaa
    15061 cgacaatatc accctgttgg ccaaaggtgt cttgctcaga ggcgagattc gcgttgaagg
    15121 cacggtccgt atcgacgggc ggttggaagg cgatcttcac accacgggga ccgtcgtgat
    15181 cggcgaagac ggcatcgtgc agggaaccat cacagccggg atcatcatca gcagtggaaa
    15241 gatcaaggcg acggtgcggg cgacggaacg gctccaattg ctcaagaccg gtctgctcgt
    15301 cggcgaagtc cacgcgcccg ccttctccat ggaagacggg gcgaagtttc aagggatgtc
    15361 ggatatgggc gtcgcttcct ggggtgacga agcccagaaa ctccccggca atgttcggga
    15421 tatttcctcg cagcgtccca aggccgtcgc catgctcggt gaaaacgcct gacggctcgc
    15481 tttcccgccc tcacgctgcg caccccgtgc gccctatggc atcgtagggt ctcctcccag
    15541 cctggcggca gtcctctgtt caggcacagc ggtcgtcaga ccgacgcgtg acatcctgga
    15601 caggttctgc tatcaacggc tgcaatcgca ggtactcttc acgactatga atcgacaaca
    15661 gatttttgcg ctctgctttt tcggcgtgct gctggcgctg ctgtatcaga tggggctgat
    15721 gtttcgcccc ttcgtgtttc cggtcctctg ggcagtgatt cttgcgcatc tgtgtttccc
    15781 cctgcacatc cggctcaccg cctggttggg agggcgggag tcgcattcgg ctgtgctcct
    15841 gacattggct gcgctggccc tggtcgtggt gccgctggtg gtgctgagtg taatgctggt
    15901 gaaggaggcc ggatccgctg accgcgcggt gcgggaatgg attgcctcag gaggcgttca
    15961 gcagttgccc gatcggttgt ccgggctacc gggaggccct gtggtgaggg agtggctgga
    16021 gcggttcacc ggcagggaaa gtgatctcga acaattcgtg ctctcaagcg cgaagacctt
    16081 cagtagattc atcgtgggtg agttgagcga ccttgtgcgg gacgtcttta tgttggtcgc
    16141 cgactttctc gtcatgatgt ttaccctgtt tttcttcttc aaagatggcc gccactggct
    16201 ggcgtcgctg tatgcgttga ttcccctgga agcctcgcac aaggacaaga tcctggctcg
    16261 cctggatcag accattcgcg ccgtggtgaa agggatcgtg ctgacggcca ttgcgcaggg
    16321 gctactcgcc ggagcggcct atgcggtgct gggtgtgccg tttcccatgg tgttgatggc
    16381 cctgacgatt ctcctggctc cgctcccgtt cggcgggacg gcgctcatct gggggcctgt
    16441 ggccttgtat ctcctctggg ccggtacggt ggggaagggg ctggtcatgt tggcctgggg
    16501 aatcggggtc gtgtccacga tcgatcaaat cctcaagccg ctgttcatcg ggcagggcgc
    16561 ggagattccc gtgttgctcc tgaccttcag tgtgctcgga ggcctggcgg tctacgggat
    16621 cctcggattg ttcctgggac cgattctcgt cggactgttc ttgaccgccc tgcagatcta
    16681 ccgggacgac taccagcagc accttccggc cccgccggcc gcatcgtaat cagccgttca
    16741 accgccggtc tgtcagtcga ggggaatggt aatcgtcatg ccccccatca cgtcattcaa
    16801 cgcaaagtca cgtttggggc tcagcgggtg gctgttgtga cacttcacgc aggcggctga
    16861 gatggcccga tcggaataga tggcctgaaa gtattgtttg cggccgctgg tcacgatgcc
    16921 cgtaaagggc cggtcggggg tctgcgaaaa actctccagc gcccggcgtt ccaagtcggt
    16981 ggcgggggca ttccgctggt agatgggcca gaggctgatg agacgatagc gaatgcctcg
    17041 tccggactcg gccacgagtt tacccgaatg ttgaaggaac tgggcaggca gcggcagggc
    17101 attttcgtgc tcccaatgtt ccgcggcaga cacgatgcct ttggcctgca tccggttgac
    17161 gacctggttc gtgtagatgg ttcgatcggc atccagaacc gcgtgtacga agtccgcgac
    17221 cttttccgga ggaatgccag gcggcggcgc gacatctttg gcctgcaacg aatagctcga
    17281 gacgcaggcc agcgccagtg cgagccacag cggccgtgcc cgaatgactg tcaggatgag
    17341 acgcatggct cctcctttcg aaccccggcc ttgtccgccg gcagggtgat gacgcaggtc
    17401 gtgccctggc cttccggact ctccagccga atctcgccgc cgaatttctt gatcagccgt
    17461 tgcgcgacgg tgaggccgag tcccgatcct tctccctgtc ccttggtggt gtagaacgga
    17521 tcgaagactt tgccgagatg gtgtttggaa atgccgggac cgttgtcccg tatcgtgatc
    17581 gtaatcaggt cgtcctgttc ttcggtggtc agccacaggt cgccccgccc gttcatggcc
    17641 tgaatggcat tggtgaggac gttgataaat gccaggcgaa gctggtcggg cagcgccgtc
    17701 accggcgaca ccgccacata gtgtttctgg acgttcaggc cgaggcagtc gtgcgaggtc
    17761 tgtacaatgg ccagggcctg ttctaactgg gcggtcacat ccaccggcac acgttggctt
    17821 ttcgattcac gggcggccac tccggtgaag tcccgaatga tggccgccat ctttcggccg
    17881 tgtccgacga tgtcttgcgc gtaacccttg gcgcgagcca ggtccgcttc ctcctggatc
    17941 gcctccccga ggcccagaat tccaaacagc gggttgttga gttcgtggcc gatgccggcg
    18001 gtcagcacat cgaggctgcc tgatttttca gcttggatca gctggtcctg cagccggctt
    18061 tcgtcggtgg tatcgcgcag cacgaggccg aagcgcttgc ctgccccgct gcgactttcc
    18121 agcggaaacc attcatagtg gtacaggtgc gcgccgatcc gtaattcccg tctggctgag
    18181 ggggacgcct ggctgagggt ttgattgagc gggtcgcgcg cctctttcag gacgggctcc
    18241 tggtcctggc gggagggcgc ggtcggtttg ttttcttcaa cgagtcgtag gtctcgccgc
    18301 aaggcctgaa ggtgatcgtc gtccagaggg agaagatcaa agagcagacg gcccgaccag
    18361 ctttgttcct gtggctgaat ggcatcccgg gacgcgcggt tcatgtattg gacgaagcct
    18421 tgctggtcga tcatgatgat cggggtcggg acggcgtcga gcacctgatc cgtcgatttt
    18481 tggaggtact gcacctcctg tgttttttct tccacctggg tgctcaatcc ggcaaaggcg
    18541 gcttcgagtt gcgtgttcat gcggttgaat tcgaccgcca gctcttccag ctcatctccc
    18601 gtgtgaatct ggatcggctc ctgcaattct cctcgcccga tgaggcgcgc ggcctcctgc
    18661 agtcgtctga ccggcgtcac aatccggctg gcggcgaaat accccagtgt ggcgagcaag
    18721 ccgagcgcga tcccggcgag aatcatcatc cacatcatga gatgccggat cggggcgaac
    18781 agctcgtcgg aggattgcca gacaaaggtg tgccaggcgc cattttccag ggagccgttc
    18841 gtggcgcgac tggtttcggg gaggggagcg aatccgatga tggcggtgcc ctggccgccg
    18901 tgcccgtcac tgtcggccgt gacccagccc ggttgacgca ccgtcacctg gggaatcagc
    18961 gatgcgcctg agagctgaac gccggtcggc aggatcgggc agctgaggac catgcctcgg
    19021 ctatcgatga gcatcacatg gccggtcttg ccgaaccgga tggggtgggt cgagggagag
    19081 aagaactcct tggcatcgat gacccggtgg agcacgccca ccacttcata ccgaaggctg
    19141 tccatgaccg gcagggaaat gctgaagaca taggcattgg cctgttcgtc gaagtgaaga
    19201 tcttcgatgt agagctggcc gactcctctg ttgaacgagc ctttccacca gggactgtcg
    19261 gcatgggaga agcctggttt ggtcgtcagg gcggcaaaga ggtttccctg cacatccgtg
    19321 atgaacagct tcttggtggc ggcgcgcacg acctgcggca ggagttggtc cggttcgctc
    19381 tgcgagccgg cgtaaaaacc ttgcaagaga agggcggtgg aatttcccgt gatcgctttg
    19441 atcgcagccg ggtctcttgc cgcccagcgg gcccgttgtt gcacgagagt cggcaacgta
    19501 gcgttctgat cggaggcgtg gagcgcgtcg cgacggtgtt ccagcgcacg cacgatatcc
    19561 gggtggaccg cgatgcggga ggtcctggcc acctcctccg ccacgaggag gtctaatttg
    19621 cgggccgcct ccgtggccag cgcctgaaag ctttccccgc tgacgacctg gatttctttc
    19681 gatccttgga ggaaggccag gctgaggccg agcaggaggg gaaccacgcc gaccatcagc
    19741 atggagacga tgagcttacg tttcaggccc cacccgaggg ccttcgagaa ggagcgcgat
    19801 gtacttgtca taggggcgcg catgatatgg tcggctgcac ctgtggaaat tcgatgcgga
    19861 aggtcgtgcc cacgcccacg tcgctttcga cgcaaatgtg accttccatt ttcgagatca
    19921 ccgacatcac attgtacagc cccaaccccg tgcctttccc gggaggtttg gtggtgtaga
    19981 gtggttcaaa gattttcgag aaggcttcct tggggatgcc gcaccccgta tcgctgaccg
    20041 aagcgctgac cagtccgtcc ggcccgacag cggtttggag cgtcaatgtg ccgtgatcct
    20101 tgatggcttg gaccgcgttc gtgatcaaat tgacgaagac atgaaggagt tcgtcgggat
    20161 tgcccatcac gctcgcttct tccgcatacc gtttgacgac ggtcacgtcc tggagcgtca
    20221 ccgcataacg ggcgattctc agcgcttcgt ccagcttggt gttgaggttg accatgacct
    20281 cttccctgga gccgttgcgt cttgcgtatc gggtcaggtc gcgacagatg gcgctggtgc
    20341 gtttgacggc ctcggtgatg tcgcgggcct gttcatggac gaccgcgagg tcctgctcct
    20401 cttccagatt ttcggccagt ccgagaatca actgcagcgg attgttgatg tcgtgcgcga
    20461 taccggccgc gaaagagccc agtccggcca gtttgtcggc ctggaacaat tcagcttcca
    20521 atttcgactg gtgttgaacg agtttccaga gcagtctggt ggcggctccg ccgaggacct
    20581 cggagccggg acgcttcatg gtgtggatga gcggttccat ggatgggtcg ccggtcacat
    20641 ccatgatcag attgacacca tgattctgga tgagatcctg cacccgttcc gcgaccagca
    20701 cattcagatc tcgcgcccgc ttgagtccag gagccgtcgg atccttgtcc gcaatgccga
    20761 tgatctccac gtccgggatt tgatgaagga gatccaggat cgcggttccg ccttttccgg
    20821 cgccgatgat ggcaattctc gtcgcacaaa gtttcatagt cgcttcccgt gatgtgtcat
    20881 gaggtccggt ccgaggcgag cctcacccac tcgcaccttg ccgtcgggga ggcctggtat
    20941 gcctccccga cgtggagggt gtgccgccgt ctgtcggcat gggcgtcact agagctgggc
    21001 aagctcccgc tgttccatgg ctctggccac ggccgtgcgt aacttttctc cttcgaccgg
    21061 tttcacgagg tagtcgacga cgccgcttcg taagaacgag gttgccatat cggtgtcggg
    21121 gaagccggtc aggacaatga tcggcacacg cggccattcc tgctggaagt aagcaatggc
    21181 ctcaaccccg ttgatcttcg gcatgcggat gtcgcagatg aggacgtcca gcagcagccg
    21241 gttttctccg gtgttgatgg cctcgatggc tttttccccg ttctccgcct cgatgacctc
    21301 atacccggcc ttctgcaagg tcattcggac gactttgcga atgtccggtt catcatcgac
    21361 gacgagcact cgaccgttgc aggcggactc actcatgaag aaacccgatt taaattctcc
    21421 catgatcccc tccttgatgg ttgtgtcgtt tgttgtttgc atctgccgta cttgctgctg
    21481 agtaacagca acacacatgc cagtgaagga agcctgaaga aggtcgtgat ttcagtatgt
    21541 taggaaaagt aagtgaggag gggttccggc ctggtggttg agaaccacca ggccgggtca
    21601 aatgcaccac cggctcgccg atcagatcgg gaaaccggaa ccgtcgaaag gccattgggt
    21661 gttgatgagt ggaccggtcg atatccggtc cggctgaaaa aagaggcgtt acttggtcgg
    21721 ggtacttccg aagaaatgta agtccttctc gccccggaga tgtgcagggg tgacgacata
    21781 ttcgccgccc agggcctggt ttcagatctt tccggacgtc actttttcac cgtcggtgaa
    21841 ggtaactccc attcctccag cgaccgtacc gagcacggcg acggccattt tgatctcgcc
    21901 atacggcaag gtcaggaagt aacttcccaa gccctgaatg gcttccgatc cgaagcctgc
    21961 ggctgcggtc ggtcgcaccg ataaagttgc ctggcatccg aggacgatca gcaatacgca
    22021 cagagtgatt cgtgcaaggg gagggggtgg cgtgaattcg cctgaaccat gagcagtctc
    22081 ctttcatcct gtgtaagcgc gaggcatgaa ttcggtcacg caagggcgtc gtgtagcggg
    22141 ctgccattca actaccatgc gccagctgcg cgtcccgccc agcctcagcg atgaaatcgg
    22201 tggtggttca gattgccttc ggcttcccgt atgcgttatt ctccaggaag atgattaccg
    22261 ccgaagctgt gaccgcattc attcgtcagg tgtttcctga tgctgccgtg accgtgatcg
    22321 acaagaccgg cacgcaggac catttaattg tacgggtgac ttcaggcggg tttctcggca
    22381 agaatcttct ggaccgacat cggatggtat accaggcctt ggccgctccg atgaaagatg
    22441 gacgtatcca tgccctggag attaccgcca aaacaccgga agaagtgaac acatagtaat
    22501 agtagaggag ggacagatgg ctgacccgat cgaagaagag attcaacaag aaatcaaggc
    22561 gaacaagatt ctgatttacg gcaagggcac caaagccatg ccgatgtgcg ggttcacgag
    22621 ggagaccatg cactttttcg aaaaatacgg gtatccctac gagcttatcg acgtgttgtc
    22681 ccagcccgcc aaacgcgagg cgttgacgaa gctgacgaac tggccgactt tgccgaaggt
    22741 gttcatcgac ggacagtttt acggcgacac ggatattctg gaccccatgg aagccaaggg
    22801 cgaagtggtg ccgttgctga agaaggcgtt cggagctgaa taagcgcgct gagccgtcgt
    22861 ccattcctct ctccaatccg tagccggctt tcaggggcag tcggttcgat ccccgtccgg
    22921 cataccagat cttcactccc tcgaagttcg tcgtatgtgc ttgcccagat tcccccttgc
    22981 cgatagaatc cctcctgcgt cccatgagtg aggatttcca ccccaaaagt cccctgtgtg
    23041 tcgatcttga tggcacgctc atcaggaccg atctgctgtg ggaatcgctg ctggcgctgc
    23101 tgaagcagag tccgctctcg gtctttcagc ttcccttctg gttgctgaaa ggcaaagcct
    23161 ctttcaagca tgaaattgcc cgccgggtga cgctcgatgc cagcatgctc ccctacgacc
    23221 aggctctggt cgagtttctc tccaacgaac gccgggctgg ccgtgagctg gtgctggcca
    23281 cagccagtca tgagagtttt gcccgtgccg tagcggctca tctcgggctg ttcgatgagc
    23341 gggtgttcgg gagcgatgct tcgaccaatc tcaaaggtgc acgcaaggtt gccctcctgg
    23401 tagagcgata cggggctcgc cgctttgcct atgcgggaaa ttcgaccgcc gatttgcccg
    23461 tgtgggccga ggctaacgaa gcgatcatcg tgaatgcctc tgccggattg gtgagccgcg
    23521 cgcaaacgct gaccccggtg agtcgtgtgt tcagcgagcc ggtgaaatgg ctgaagcagg
    23581 tggccaaggc cttgcgcgtg catcagtggg cgaagaatgt gctggtgttt ataccggtgg
    23641 tcgcctcgca tcaaatcacc aaccgagccc tgatgctgca ggccaccctg gcattcctgt
    23701 cattcagttt ttcagcgtct gccgtctata tcgtcaatga ttgtctggac ctcgcgtccg
    23761 accgcaggca tccccggaaa aaataccgtc cctttgcctc cggcagcctc tcgattccgt
    23821 tcgggcttct cttggctgca gggtgtcttg tggggggtat tttgctggcg ttggcactgc
    23881 cctcgccatt tctgctcgtg ctggccggct acctggttct gacgacgggg tattcgttct
    23941 atctgaaaca gttcgtgctg ctggatgtga ttgtgttggc ccaactctac accgtgcggg
    24001 tatacggagg cggggctgcg acgggggtgg ctccctccca ttggctgc
//
LOCUS       DAORRQ010000059        23818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_123_length_23818_cov_36.760300, whole genome shotgun sequence.
ACCESSION   DAORRQ010000059 DAORRQ010000000
VERSION     DAORRQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123_length_23818_cov_36.760300"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            525..1157
                     /locus_tag="PK224_15910"
     CDS             525..1157
                     /locus_tag="PK224_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB17279.1"
                     /translation="MTRLGNIVAVSFLWFAAGMLHPHSAFAEWYVAGQFGANLADRLS
                     DIRGTGDLSGFRAPDFDLQNSFLYGGKVGTFPGNGWFGVELDAFHSTPHIKNLDDVPG
                     IHMRVTNVGVNLLVRYPGVTFQPYAGIGAGAAIARIGDSATTRGNTDVSSAVNVLLGM
                     RAFITPYVAVYTEYKYNQSTFEFNQAFGSASGFNGDYDAHILVTGLSYHF"
     gene            1114..1881
                     /locus_tag="PK224_15915"
     CDS             1114..1881
                     /locus_tag="PK224_15915"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HRB17280.1"
                     /translation="MMRTSWSPACPTISRNRPVWPHLLAGVAAMLLSLATGCAQSAHQ
                     SGRDLLDRILPDHVQQQVDPSVSFAALKANPSAYQGNTIMVSGIVLKSRRVKDRTEIE
                     VLQIPTGSGAVPTNDRTRSQGRFLAVKSGEFLDPAVVDAGTPVTIVGEVGPAVTRAMD
                     EGEYTYPVLEIKHLVDWDEVNPQIRFAPYAYPYGRSMYAYPYSYWGPYGGLGGWYGGY
                     PGYGYGYPYYGPFYGIGSGSSAPSGPPPASAPPQFRK"
     gene            1915..4527
                     /gene="ligD"
                     /locus_tag="PK224_15920"
     CDS             1915..4527
                     /gene="ligD"
                     /locus_tag="PK224_15920"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HRB17281.1"
                     /translation="MGLQQYWKKRNFQRTPEPRGKNVPPDGRLRFVIQKHAASRLHYD
                     FRLELNGTLKSWAVPKGPSLDPAQKRLAVHVEDHPLEYAGFEGVIPEKQYGGGTVLLW
                     DRGYWEPIGDPGSSYRRGRLTFTLHGEKLHGIWNLVRMGGRKEDGKENWLLIKEDDEE
                     AQRGKRGDITQTLEESVASGKTLEQIAAGEPAVWQPERPSGKKSRAGSAPRPIKRTPA
                     PGARKAPLAPWMAPQLATLVPTSPEGPDWVHELKYDGYRMLCRVKDGTATLLTRNGHD
                     WTAKLPHVAAAAADLRINSAWLDGEVVALLPDGRVSFQRLQNAFDAQTDSHLVYVVFD
                     LLYLDGYDLRPIPLIERKRLLSALLQDNSQSTLIRYSAHIAGQGEVAFAEACRSGMEG
                     LIAKRADASYLAGRNRNWVKVKCGRRQEFVIGGFTDPSGSRTVFGALLLGVFDENGRL
                     RYAGRTGTGFSNRSLAELHARLKRLQRTKSPFVNPPGGSQAAGVHWVKPTLVAEVAFA
                     EWTNEGQLRHASFQGLREDKDAGSVVAERTSENASSEKPGQPNDRTKRTGRKNRAAQG
                     DSKRASSAQATVVGGVTLSHPDRVLFPEQAVTKLALARYYEAVRDWILPHVKDRPLTL
                     VRCPEGYQKDCFYQKHANDQMPDVIGRVEVPADQGSACYTIAHSLEALLGLVQIGVLE
                     IHTWGAKRDKLDRPDRMILDLDPGPAVDWSSVIEGAQLLRTLLRELNLTSFVKTTGGK
                     GLHVVVPLQRVHTWDEVKTFSKSLAEHLERLIPERFVATMSKAKRQGKIYVDYLRNAQ
                     GATAVAAYSTRARPGAPVSVPLAWDELSIDLRSDSFTLVNLPDRLRRLSKAPWAEYGA
                     TKQRLTKTMRLRLT"
     gene            4676..4936
                     /locus_tag="PK224_15925"
     CDS             4676..4936
                     /locus_tag="PK224_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17282.1"
                     /translation="MNSTSMTIPLINRSRQTAESVRGSSSCCERCGGLMIGERCMDIG
                     ESLGGYWFWAMRCIQCGDIVDEVILRNRHLPQERPQEVVRAA"
     gene            complement(4973..5344)
                     /locus_tag="PK224_15930"
     CDS             complement(4973..5344)
                     /locus_tag="PK224_15930"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HRB17283.1"
                     /translation="MEAIGRRRWAIAEGYLPAGSHGPAPQMTSHETVCILNTSDQHAD
                     VRITIFYADREPVGPYLVAVPARRTRHVRFNDLKDPAPIPTDTDYASVIESNVRIVVQ
                     HTRLDSRQAENALFSTMAFPA"
     gene            complement(5387..7138)
                     /locus_tag="PK224_15935"
     CDS             complement(5387..7138)
                     /locus_tag="PK224_15935"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRB17284.1"
                     /translation="MSSTVADLLIDRLIAWGVDTVFSLPGDGINGIYEALRTRHDRIR
                     LVQVRHEESAALAACGYAKFTRRLGVCLATSGPGGIHLLNGLYDAKSDGQPVLAITGH
                     TFHDLIGTHYQQDVSLDKLFSDVALYSERVMGPTHVRNVVDEAIKTAISRRTVAHITI
                     PKDIQDWSAQEEGSKANIPGHSGDRYSDPLPLPSQSLIERAAAVINAGSKPAILAGRG
                     SLGAKSEILELAELLGAPIVKPLLGKAVVPDDHPLTTGGIGLLGTAPSQEVLETCDTL
                     IIAGSSFPYLEFYPKPGQARAVQIDLDASRIGLRYPVEVGLVGHCWDVLRALLPLVTH
                     RSDRTFLTDAQGRMAQWNTLMEERGSRMDMPLKPQVVVRAVNEFLADDAIVCCDTGTV
                     TTWVARHIKMKDQMEFSASGTLATMGNGLPYSLGAGIAHPGRQIVCIAGDGGFTMLMG
                     ELATLVKYALPVKIIVLKNNVLGMIKWEQLAFEGNPQYGVDLQPIDFAGFARACGATG
                     FTVEDPRQVQDVLRQAFSTPGPVVVEAVIDPLEAPLPGKITTDQAWQFAKALARGQDD
                     RWEIIKTLVTGKIREVV"
     gene            complement(7138..8337)
                     /locus_tag="PK224_15940"
     CDS             complement(7138..8337)
                     /locus_tag="PK224_15940"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRB17285.1"
                     /translation="MTPVYPDPLIKQEVPLPPGEQVSPAPADRHLWQITPIRDMMWLG
                     GILFCLWFGYYLRGVFTPVIIALLLAYLFNPLIRRAKDRWHIPRPVTISCILFVSAVV
                     ILGLITWLGPLLAEQVQSFAERVPSYIQSIAQRYHVRLGDFSEHLSGLATSLKDDPLS
                     ILRPVFSGTGQAFGVLGTVIGTTTDVVLAFILIPIYFFFFAWHFDGTLDQIKRYIPTT
                     YRSRVRHIVRRMDDAVSGFFRGRLAIALGSVVLYSLAWAFTGIRYWFLLGLITGILTI
                     IPYASLIGWPLAIALKYLDVLSSNAGGFDLMTIVIWPSLAYLLVQFIESWLLTPWVQS
                     QSMDMNAVTVLIVVFIGGALGGFYGLLLAIPIAACLKILAQELLLPRLARTAILSGPL
                     DEQRRAR"
     gene            complement(8372..9301)
                     /locus_tag="PK224_15945"
     CDS             complement(8372..9301)
                     /locus_tag="PK224_15945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRB17286.1"
                     /translation="MPTSLTGSSPGSNPWKLGGLGWKALVRRLWRESLHDDILGRAAQ
                     LAYYFLLALFPALLFLTAFMGLFPLKQTMPELMQYLRTVLPADALSLLEKYLDNVMKG
                     SSGDILSLGLLGALWASSSGVTAIMEALNVVYGAKETRPYWKVRLVASSLTVGLAGFI
                     IVSITLILYGARIGEWIADMVGLGWLFLMTWNIAQWPVAVTLMLLALAIIYYVCPDVK
                     QDWRWVTPGSVCAGSLWLLVSLAFKAYVEHFGNYNAAYGSIAGVIVLMLWLYLTGVVI
                     LLGGEINAQIQQAASSLRIRQEQAPQPVPAPAN"
     gene            complement(9434..10156)
                     /locus_tag="PK224_15950"
     CDS             complement(9434..10156)
                     /locus_tag="PK224_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17287.1"
                     /translation="MDQRTSAIDQDLKDIAQTRIAIAEKWDALEQQITHRVEDVTMTC
                     SDMLNRATDNIGAFVNKTKAVVDPVHRIDRHPWLMLGGALCAGYAIGLIEQGTRHQRR
                     GVYPHYPPGVRGPRVMPAPAQRNAESRQSEGVYDYYPHESQSSFRETGGNGSNLWSSL
                     SREFGPDTEEAKHMVLQVGRTLLFELARKAMPEIARTLGVTLSALTPKDEYSSRTQST
                     SNKGTDGGNSRSNDAQPSAQAT"
     gene            complement(10120..10527)
                     /locus_tag="PK224_15955"
     CDS             complement(10120..10527)
                     /locus_tag="PK224_15955"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRB17288.1"
                     /translation="MDNPHPISIGALVSGLMSDVRCLLRQEMELARHEMQHERHKLSS
                     IMIQSGIGLLLGMMAILFLLMMVVHLLVTYTNLPLWACFGIVGFISALGTGGLLYRVA
                     RIGAGLRLWPFRTFHSLKEDIRWIKERVLSTRT"
     gene            complement(10547..10897)
                     /locus_tag="PK224_15960"
     CDS             complement(10547..10897)
                     /locus_tag="PK224_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17289.1"
                     /translation="MDQEKSTLEHTAEQMKATVHDAVSDTMAAADRTTKRAGEALESS
                     ADRLRERLPQDGAAGAAADAVSRGVKQTSRVLQQQGVSGIVEDLEILMRRYPLQTLLV
                     GLGCGYLLARTRPE"
     gene            complement(11039..12142)
                     /locus_tag="PK224_15965"
     CDS             complement(11039..12142)
                     /locus_tag="PK224_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRB17290.1"
                     /translation="MSLSPLRFAVVGLGHISQVAVLPAFAHAKSSARLTALVSDDPLK
                     RRLLGRQYGVAHTYAYKDYEQCLRSGEIDAVYIALPNSLHREYAVRAARAGIHILCEK
                     PLAVTAADCRKMVRAAERYRVKLMVAYRLHFEACNLQTIALLQSGRLGEPRLFHSVFT
                     QQVRPGDIRLSQRLGGGPLYDIGIYCINAARYLFRDEPTEVTALVGRGHDRRFQKVEE
                     SAGAILRFPHDRLATLTCSFGATDAAMYEIVGTKGRVRVEPAYEYLGSLKASITVNGK
                     TRVRTFKPGDQFAPQLIYFASCVQDNRQPEPNGLEGMLDVQIIEALHRSARQGRSVPL
                     RLSTKQVRPDVRLQMHRPPVVKPKQVRATSPTL"
     gene            complement(12252..12650)
                     /locus_tag="PK224_15970"
     CDS             complement(12252..12650)
                     /locus_tag="PK224_15970"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB17291.1"
                     /translation="MANTPTISNTDGAVKLVRDDHRHILALFQLYRATPADSRQSYIE
                     QILQRLSDHFHMEERLTEDVRHHGNEGRILVEQLLVEHEEIKAMIDELQQAENDDDES
                     LDAFFEDMMQTVRAHFIAEERDLFPLLNKG"
     gene            complement(12827..14302)
                     /locus_tag="PK224_15975"
     CDS             complement(12827..14302)
                     /locus_tag="PK224_15975"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRB17292.1"
                     /translation="MGRYTQPVLPALCAVLLLGNACSHSIPQSTSKPVPPPITSAVPS
                     FAAMAKEESRAPLSQMPFQVKTDLTPIQTAIRDAIPERITEAGHPLGQEFRWTFVRSS
                     NPQVHIQDGLVAIHAEYKGEIESRGSSRACRLDPVFATLDVTGKLALLQESESVSFGF
                     DPTHLAVRTKPESDARCNMFNVPVTDQAPDLFGLPEIKIALTDAVHADAFAIPLQRLW
                     DDLDGPLSLSMATLNTHACLYGNPREMILGQQKGTTQDTVILGSAKQMPFITYEPTCT
                     EAAPTVALVNSGPLSAVNKPYTMLARVPFSYQQLSHQLQSKLFHQTVVLDSTASDRAV
                     IEQVSAADASGRVLVTVELSGDLKGTIYYWGTPRLDDGGRSLSVPDLQMANESKSAID
                     SIRIGYWQLVDRELNQKLRQAMAVDISAQVDRLKQTLTGTHRSGGVTMDILVTRQLPD
                     QVRSSPQGLTVIILLEGTANATGRVTLEGQRTRALLPRESR"
     gene            complement(14594..14908)
                     /locus_tag="PK224_15980"
     CDS             complement(14594..14908)
                     /locus_tag="PK224_15980"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRB17293.1"
                     /translation="MIAHLTRFVFAVTMLSLVGCQSTTGKTAGQTIDDATITASVQSK
                     LSSDRLSNFSRIDVDTERGVVTLNGVVTSTEQKMRVAELTRGVTGVRTVNNNLQIQPQ
                     PQ"
     gene            complement(14979..15305)
                     /locus_tag="PK224_15985"
     CDS             complement(14979..15305)
                     /locus_tag="PK224_15985"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRB17294.1"
                     /translation="MHTEESSNWSAFMAGAFIGAGIALLLAPQSGTELRSTLRDYASK
                     AKDELDEATEQGRAAWDHAVDRGQEYVEGGKQTLRNAGRAAREFVDETVQTAEKTVGE
                     AASRRA"
     gene            16316..17479
                     /locus_tag="PK224_15990"
     CDS             16316..17479
                     /locus_tag="PK224_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRB17295.1"
                     /translation="MVKLTKKLIDAAPFPKQGQMFLRDAEVRGLALRITTGAKAFVFE
                     KKINGRARRFTLGPYGALTVEAARKMAQEKIGEVAKGDDPAEARRQRRQATTWGDLET
                     LYLERHAIHKKSMKDDVSLLNRHLAQWRTWRLSAITKAEVCSKHAEIGAAGHRIAANR
                     MIALVRVMFNLATDWGFFAGVNPAARVKLFKEVSRERFIKPDELPRLWKALGDESNPY
                     VRVAFMVGLLTGARRNEVLAMQWTDLDVSQGLWVIPSTKAGRMHTIPLPRPALEALIG
                     LPRLEGNPYVFCGRWGRGHLVNVAKPWRRIRQQAGLNDVRIHDLRRTLGSWLVAAGAS
                     LPLIGKALNHSNVSTTQVYARLQLDPVRVALEDNAARMLTIVNEAHEVEHAEG"
     gene            17466..18305
                     /locus_tag="PK224_15995"
     CDS             17466..18305
                     /locus_tag="PK224_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17296.1"
                     /translation="MPKGKGKNGPKKSPSKPTRRQSSESLSAQNAKAVAKELEENFMM
                     SCMAAEALPVLLAPLIDPYATMLLELIEQRQPKSTSDPLLQLHVLHRRVLQIPDETHP
                     IDADAALLLAGEVGIQAPAWAVDLARKVRLEKYSSRDMDLNKTLGFTAEGKGRTAEIE
                     RRARVAIVFRSMLNILGLRLIGVSMEDACMLEAGRMQRLGKSWNKSAYDMVPDLGSHV
                     RNPEKRDSELRDKRSRYAEKLRQEWYAWKSPMKDSDSPESIALKTDIEADKAKFLAQF
                     PPL"
     gene            18898..20949
                     /locus_tag="PK224_16000"
     CDS             18898..20949
                     /locus_tag="PK224_16000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HRB17297.1"
                     /translation="MLYLSRLIEWRSIANDYQKLITITEGEKKSLAACQLGLPCVGVA
                     GVWNWRARLDDGERMALPGLDQFTWKGRTVELIPDSDAWRPNKEGDILAGFYALGHEL
                     KGRGAFVSFVELPEYNGVKCGLDDFIVRAGAFPLETFQGCKRYALDHPRFKALSAWHQ
                     KWERRQRGGNQGLVKTIADEILKTEHFAKDAGGELFAYEAGVYRPRGGERIAQCVKRL
                     LDMSADSARWSSHLAREVVEYVRIDAPSLLERPRPDTLNLRNGLLDILSGTLRPHEPG
                     HLSSIQLPVAFDPSAMCPQWEAFITRVLPEDCRNIICEIMASSMRGDISDQKAILLQG
                     SGENGKSTLLDAVIAFLGRENVSSLALQRLEVDKFAAVRLLGKLANVCADLPADHLAS
                     TSTFKALTGGDILTGERKFQGSFEFASFARLIFSTNHYPQSKDASHAFFRRWLVVPFD
                     AVIDPRERIPNLAAKLADAQELSGILNRVLAVLPGMIQRGGFSQSETTQAAMMEFREM
                     TDPLAAWLDRCTVLSPEQLVSRKDLLISYNAQAEASGRPALTSKAFCQAIRRLRPTIS
                     EAQRTVCGAMQWVFLGLGLAASPPSPSHNSHHSHDSSQISLEVETDKGEVNKNLKRGN
                     GVNHVSPVNEAVATIIPCFNCRGERFWESIHGARACATCHPPVNPGLVREWIENAPVN
                     Q"
     gene            21153..21593
                     /locus_tag="PK224_16005"
     CDS             21153..21593
                     /locus_tag="PK224_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17298.1"
                     /translation="MTPREVVQAIAAVMERQEAIQNTQLLILRELRIMRCGAGRVDED
                     SLEALLGYLYDEFHNDTWTSRVAFDQAAENRALYAALVRCLGEEGTPNGLGKLLKNNL
                     GKTGDYSLECVNKHSNAGAVFRVTVSVTSSPLKPSMMEEQRSTS"
     gene            21590..22708
                     /locus_tag="PK224_16010"
     CDS             21590..22708
                     /locus_tag="PK224_16010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRB17299.1"
                     /translation="MTHETTLSDLHHSSIPCPDAFLSAKERRKYSILKPLRALVLGAQ
                     NPHFFEEASLELEASREVARRHSREASTSFFVPAEVLQRPISQEAYSRAMATTPGSKG
                     GYLVNTANMGFIDVLRNRSVSMAMGARVLSGLVGNVSFTRQTGKATVTWQAGEGVSAS
                     ASDQALGQITMSPKFCISITDVSLQLLSQTSPAAESFIMADLARDVAIDGVDAAVING
                     TGGAQPLGVKNTPGVNTGQDASSATYAKILAFPTVAGTANALRGNPGFVTNTVGAGKL
                     MGVQRFSNTDSPIWEGNIMNGELVGFPAMSSEQLASGNLIFGSWEEILIGEWGVLELA
                     TDRGGDRFNKAQVGIRAMWMVDVAIRYPQAFVVGTNLS"
BASE COUNT         5090 a         6564 c         7118 g         5046 t
ORIGIN      
        1 gagcgagatt ttcgatgcgg aacctcccta ccatccacgc gggtgcatcg cccaagcctg
       61 gagcgtcgca gagcttttgc gggtctgcag aaaaacaaaa caggccctgg agcctcaatg
      121 aacacctgga tctcccgtgg gccgggccga atatgcagga cggcgtagca catgccccat
      181 gtgcgcatcg gcacatcggg atggcactgt caacattggc gcggacccta ctatcgctcg
      241 cgaggcaaag gaatgacctc actcttttca tcttgccatc caggcgtttc taatgcgatg
      301 agcccttcgt cgtcgttcgt tcatccttgg acccgatttg aagagagcct ccgtttctcc
      361 ggcccatccc gtttcgcaca tcatgcacct ctgaagtcgg ataggaaaaa cgccgaggct
      421 agggtaaacg ccagctacca cttgctgttc cgtaccggta cagtcggctt ctgcgcctgt
      481 ctcgattgag ctcaagcccg ataaatccag agagaccacg atctatgacg cggttaggca
      541 atatagtggc ggtgagcttt ttgtggtttg cggcgggcat gctacaccct cattcggctt
      601 tcgcagaatg gtatgtggcg ggccagttcg gcgcgaacct ggctgaccgg ctcagcgaca
      661 ttcgcgggac cggcgatcta agcggatttc gtgcgccgga tttcgatctt cagaattcgt
      721 ttctctacgg gggaaaagtg gggacgtttc ccggtaacgg ctggttcgga gtggaactcg
      781 acgccttcca tagcacgccg cacatcaaga atctggatga tgtgcccgga attcatatgc
      841 gggtcacgaa tgtcggggtg aatttactgg tgcgttaccc aggggtgacg tttcagccct
      901 atgctgggat cggcgccggc gcggcgattg cgcgcatcgg agattcggcg acgacgcgtg
      961 ggaataccga cgtgtcttcg gcggtgaacg tgctgctggg aatgagagcg ttcatcaccc
     1021 cttacgtggc ggtgtatacc gagtacaaat ataatcagtc caccttcgag ttcaaccagg
     1081 cattcggatc ggcgagtggg tttaacggag attatgatgc gcacatcctg gtcaccggcc
     1141 tgtcctacca tttctagaaa ccgaccggtc tggccgcatc ttctggccgg cgtggcggct
     1201 atgctgttat cgctcgcgac cggctgtgcc caaagcgctc accaatcagg ccgagatctg
     1261 ctcgatcgaa tcttgccgga ccacgtgcag caacaagtcg acccgtcagt ctcgttcgcc
     1321 gcattgaaag ccaatccatc cgcctatcaa ggcaatacca tcatggtcag cggcatcgtg
     1381 ctcaagagca ggcgagtgaa ggatcgtacc gagatcgagg tactgcaaat tcctacgggc
     1441 tccggcgcgg tgccgaccaa cgatcgcacg aggtcccagg gccgatttct ggcggtcaag
     1501 agcggagagt ttttggatcc cgcggtcgtc gatgccggca caccggtgac gatcgtcggt
     1561 gaggtgggac ctgccgtgac gcgagcgatg gatgaagggg agtataccta tccggtgctt
     1621 gagatcaaac atctggtgga ttgggatgaa gtcaatccac aaatacggtt cgctccctac
     1681 gcctatccct atgggcgctc catgtatgcc tacccctatt cctactgggg gccatacgga
     1741 ggattgggag gttggtatgg cggttatcct gggtacggat atgggtatcc ctactatgga
     1801 ccgttctacg ggatcggcag cggttcatcc gcccccagcg ggccaccacc cgcatctgcg
     1861 ccgccacagt tcaggaaatg agctggaggg gccgtcatca ggcggaggaa tcccatgggg
     1921 cttcagcaat attggaagaa gcgaaacttc cagaggacgc cggagcctcg gggtaagaac
     1981 gtcccgcctg acggtcggct gcggttcgtc atccaaaagc atgccgcatc ccgtctccac
     2041 tatgattttc gtctcgaact gaatggcaca ctgaaaagtt gggcggttcc gaaagggccc
     2101 agtctggatc cggcgcaaaa gcgcctggct gtgcatgtgg aggatcatcc gctggagtat
     2161 gccgggttcg agggggtcat tcccgagaaa cagtatggtg gcggcacggt gctgctttgg
     2221 gaccggggct actgggagcc gatcggtgac cccggttcca gttatcgccg tggacgcctc
     2281 acgttcaccc tgcacgggga aaagttgcat ggcatctgga atctggtgcg catgggtggt
     2341 cgaaaggaag acggtaagga aaattggctc ctcatcaaag aggatgatga agaggcgcaa
     2401 cgcgggaaac ggggcgacat tacgcagacc cttgaggaga gtgtggcgag cggaaaaacc
     2461 ctggagcaga ttgccgcggg agaacctgca gtgtggcagc cggagcgacc atcggggaaa
     2521 aagagtcggg ccgggtccgc gcctcgtcct atcaagagaa ctcctgctcc tggagctcga
     2581 aaagccccgc tggcgccatg gatggctccg cagctggcga ccctggtccc gacttctcct
     2641 gaggggcctg attgggtcca cgaactcaag tatgacggct atcgaatgct ctgtcgggtg
     2701 aaagacggca cagcaacgtt gctgactaga aacggccatg actggaccgc gaagctgccc
     2761 catgtcgccg cagcggcggc agacctccgg atcaacagcg cctggcttga cggtgaagtc
     2821 gtcgcgttgc ttccggatgg tcgggtcagc tttcaacggt tacaaaatgc attcgatgca
     2881 cagaccgaca gtcacctggt ctatgtcgtc ttcgatctgc tctatctcga tgggtacgat
     2941 cttcggccga tccccctgat tgagcgcaaa cgtctcctgt cagctctcct tcaagacaac
     3001 tcacagtcca cgctgattcg atatagcgcc cacatcgccg ggcagggcga ggtggccttt
     3061 gccgaggcct gtcgaagcgg tatggaggga ctgattgcca aacgcgccga cgcctcctat
     3121 ctcgcagggc gaaatcgcaa ttgggtgaag gtcaaatgtg gccggcggca ggaattcgtc
     3181 atcggcggat tcaccgatcc ctccggatcg cggaccgtgt tcggtgcgtt gctgctgggt
     3241 gtgttcgatg agaatggtcg attgcgatat gccggacgaa ccgggacggg cttttcaaac
     3301 cgttcccttg cggaactgca cgctcgcttg aaacgattac aacgaacgaa atcacctttc
     3361 gtgaatccac cgggcgggag tcaggctgcg ggagtgcatt gggtgaagcc gacgctggtc
     3421 gccgaagtcg catttgccga gtggaccaat gaaggtcaat tgcggcacgc ctccttccaa
     3481 ggcttgcgtg aggacaaaga cgctggttcc gtcgttgccg agcgcacatc ggagaacgct
     3541 tcgagtgaga agcccggaca accgaacgat cgcacgaaga gaactgggcg aaagaaccgc
     3601 gccgctcaag gggattcaaa aagggcttca tctgctcaag cgaccgtggt cgggggagta
     3661 accctgagtc atccggaccg cgtgctgttt ccggagcaag ccgtcaccaa gctggcgctc
     3721 gccaggtact atgaggccgt ccgcgattgg atccttccgc acgtgaagga ccgtccgttg
     3781 acgctcgtgc gttgtcctga agggtatcaa aaagattgtt tttatcagaa acatgcgaat
     3841 gatcagatgc ctgatgtgat cggaagagtg gaggttcctg cagatcaagg gagtgcctgc
     3901 tatacgatcg cccactcgct cgaggcgttg ctgggactgg ttcagatcgg tgtgctggag
     3961 attcatacct ggggggcgaa gcgggataaa ctggatcgtc ccgaccggat gatcctcgac
     4021 ctggatcctg gtcccgctgt ggactggtcg tccgtcatcg aaggagccca actacttcgt
     4081 acgttgttgc gcgaattgaa tctgacctcc ttcgtcaaaa ccacgggcgg gaaaggtctg
     4141 catgtcgtcg tgccgttgca gcgcgtgcac acctgggatg aggtcaagac gttctcgaaa
     4201 tctctcgcgg aacatctcga acgcctgatt cccgaacggt tcgtggccac catgtcgaag
     4261 gccaagcgcc agggaaagat ctatgtcgat tacctgcgca atgcgcaggg cgcgacggcc
     4321 gtggccgcct attccacgcg cgccaggccg ggcgcgccgg tgtcagtgcc gctggcatgg
     4381 gacgaactct cgatcgacct gcggtccgac agtttcacgc tcgtcaatct gccggatcga
     4441 ctccgccgac tgtccaaagc cccgtgggca gagtacggtg cgacgaaaca acgactcacg
     4501 aaaacaatgc ggcttagact cacctagtgc atgaggatcg tggccagggt gctttcaacc
     4561 gggacctggg gggagtgcta gtggagtgat tcagggcaga ctgatattgg tgagcatgac
     4621 aaacggtggg agcgcccgcc gatcccgtca atgatcacaa tgaaaggaga gccgtatgaa
     4681 ctccaccagc atgaccatcc cactcatcaa tagaagcagg cagaccgccg aatctgtccg
     4741 aggttcatcg tcctgctgcg aacggtgcgg aggcttgatg atcggcgagc gttgtatgga
     4801 catcggcgag agtctcggcg gctactggtt ctgggccatg cgttgcattc aatgcggcga
     4861 catcgtcgac gaggtgatcc tgcgaaaccg acacctgccg caggaaaggc cgcaggaagt
     4921 ggtacgagcg gcgtgaggat aaaaacggag cgagcgggac ggtaacagcg aatcaggcgg
     4981 ggaacgccat cgtgctgaac aatgcgttct ctgcctggcg tgagtcgagc cgggtatgct
     5041 ggacgacgat gcgcacattc gactcaatga cgctggcata atccgtatcc gttggaatgg
     5101 gtgcggggtc tttcagatcg ttgaatcgaa cgtgtctggt gcgtcgggca ggaaccgcga
     5161 ccagataggg accaaccggt tcccggtccg cgtagaagat cgtgatccgg acgtcggcgt
     5221 gctggtcgga cgtgttgaga atgcagaccg tttcatgact cgtcatctgc ggcgccggcc
     5281 catgactccc ggcgggcagg tatccttctg caattgccca tcgtctgcga ccgattgctt
     5341 ccatctcgat cctccttgct agcccgctcc gtcgccgtgg gtattgttag accacttccc
     5401 tgattttccc ggtcaccagg gtcttgataa tctcccagcg gtcgtcctgc ccacgggcta
     5461 acgccttggc aaactgccag gcctggtcgg tggtgatttt tcccggtagc ggcgcttcca
     5521 gtgggtcgat caccgcttcc acgacgaccg ggcccggtgt cgaaaacgcc tgtcgcagca
     5581 cgtcctgaac ctggcgggga tcttcgaccg tgaagcccgt tgcgccacaa gccctcgcaa
     5641 agccggcaaa gtcgatcggc tgcaggtcga ccccgtattg cgggtttccc tcaaacgcca
     5701 gctgttccca tttgatcatg cccagtacat tgttcttcag gacgataatt ttgaccggca
     5761 gcgcgtattt caccagcgtc gccagttcgc ccatcaacat ggtgaatccc ccgtcgcccg
     5821 cgatgcagac gatttgtctc ccggggtgtg cgatgccggc tcccaaactg taaggcagtc
     5881 cgttgcccat cgtggccagt gtcccggagg ccgaaaactc catttggtct ttcatcttga
     5941 tgtgtcgcgc gacccaggtg gtgacggtgc cggtatcaca acagacgatg gcgtcgtcgg
     6001 caaggaattc gttgaccgcg cggaccacga cttgaggctt caatggcatg tccatgcggc
     6061 tgcctcgttc ctccatcaac gtgttccatt gcgccatccg cccctgagcg tccgtcaaga
     6121 atgtgcggtc ggatctgtgc gtcacgagtg gaagcagtgc gcggaggaca tcccagcaat
     6181 gtccgaccaa tcccacctcc accggatatc gaaggccgat gcggctcgca tcgaggtcga
     6241 tttgaacggc cctggcttga ccgggcttgg ggtaaaactc cagataaggg aaactgctgc
     6301 cggcgatgat cagggtgtcg cacgtttcga ggacttcctg tgagggcgcg gtgccgagca
     6361 ggccgattcc cccggtggtc aagggatgat catccggcac gacggctttt cccagcagcg
     6421 gcttcacaat cggtgccccg agtagctctg cgagttcgag aatttcagat ttcgcaccca
     6481 ggctgccgcg tcctgccagg atggcgggct tcgacccggc attgatgacg gcggcggccc
     6541 gctcaatcag cgattgcgag gggagcggca agggatcgct gtagcgatct ccgctgtgtc
     6601 ccgggatgtt ggccttggag ccctcctcct gtgcgctcca atcctgaata tccttcggga
     6661 tggtgatgtg ggccacggtg cgacgggaga tggcggtctt gatggcctca tcgacaacgt
     6721 tgcggacatg cgttggcccc atgacccgtt cgctatacag ggcgacatcg ctgaacagtt
     6781 tgtccaggct cacgtcctgt tgatagtgcg tcccgatcaa gtcgtggaag gtgtgcccgg
     6841 taatcgccag caccggttga ccgtcgctct tggcgtcgta caatccattc agcaagtgaa
     6901 tgccgcccgg acccgacgtg gccagacaga cgcctaaccg gcgtgtgaat ttggcatatc
     6961 cgcaggcagc cagggcggcc gactcttcat ggcgcacctg gacgagtctg atgcgatcgt
     7021 gccgggtgcg gagggcttca tagatgccgt tgatcccgtc accgggcagg ctgaagaccg
     7081 tgtcgactcc ccaggcgatc aaacggtcga ttaacaggtc cgcgacggtg ctgctcatta
     7141 tctcgccctc ctctgttcgt cgagcggccc ggataaaatg gccgtgcggg cgagccgcgg
     7201 aagaagcagt tcctgcgcca ggatcttcaa gcaggccgct atgggaatgg cgagcagcag
     7261 tccgtagaag ccgcccaagg ccccaccgat gaagaccacg atcaagaccg tgacggcatt
     7321 catgtccatc gactgactct gtacccaggg ggtcaggagc cagctctcga tgaactgcac
     7381 caggaggtag gcgagagaag gccaaatcac gatcgtcatc agatcgaatc cgcccgcatt
     7441 ggacgagagg acgtccaggt atttcagggc aatggcaagc ggccagccga tcagtgaggc
     7501 atagggaatg atcgtcaaga tgccagtgat caaaccgaga agaaaccaat aacgtatacc
     7561 ggtgaaggcc caggcgagtg agtacaggac caccgacccg agggcgatcg ccaggcggcc
     7621 gcgaaagaat ccgctgaccg catcatccat gcgtcgcaca atgtgacgca ctcgtgatcg
     7681 ataggtcgtc gggatgtatc gtttgatttg atcgagcgtg ccgtcgaagt gccaggcaaa
     7741 gaaaaagaaa taaatcggaa tcaggatgaa tgccaggacg acgtccgttg tggtgccgat
     7801 aacggtcccc agcacaccaa aggcctggcc ggttccggag aagaccggtc tcaggattga
     7861 aagtggatcg tccttgaggc tcgtggcaag ccccgagagg tgctcagaga agtcacccag
     7921 gcggacatgg tagcgctggg caatgctttg gatatacgac gggacccgtt cggcaaacga
     7981 ctgcacctgc tccgccagga gcgggcccag ccatgtgatc agtccgagga tcaccacggc
     8041 tgaaacgaaa agaatgcagg aaatcgtgac ggggcgggga atgtgccagc ggtctttcgc
     8101 tcgcctgatc agcgggttga agaggtatgc caacaacagg gcgatgatga ccggcgtaaa
     8161 cacgccgcgc agatagtagc cgaaccagag gcagaacaga atcccaccca accacatcat
     8221 gtctctgatc ggtgtgatct gccacaaatg ccgatccgca ggagcagggg agacctgttc
     8281 tcccggtggc aaaggcacct cttgttttat cagcgggtca ggatagacgg gtgtcataga
     8341 acatcgtgtc cattctgtgt gagccgttca ttcaattcgc aggggccggg acaggttggg
     8401 gtgcctgttc ctgccggatg cggagcgatg aggcggcctg ttgaatctgg gcattgattt
     8461 ctcctccgag caggatcacc accccggtga gatagagcca taacattaag acgatcaccc
     8521 ctgcgatgga gccataggct gcgttgtagt ttccgaaatg ctcgacgtag gccttaaacg
     8581 ccagggacac caggagccag agcgaccccg cgcagacaga tcctggcgtg acccaacgcc
     8641 agtcttgctt cacatccgga cagacgtaat aaataatcgc gagagccagg agcatcagtg
     8701 tgaccgcgac cggccattgt gcaatgttcc acgtcatcaa gaagagccat ccgagcccca
     8761 ccatatcggc tatccattcg ccgatgcggg cgccatagag aatcagcgtg atggaaacaa
     8821 taatgaaccc cgcgaggccg accgtaagcg acgaggctac cagccttacc ttccaatacg
     8881 gcctggtctc cttggctcca tagaccacgt tgagcgcctc catgatggcc gtcacaccgc
     8941 ttgaggaggc ccagagggcg cctaagagtc caagggacag gatgtcgcca ctgctgccct
     9001 tcatgacgtt atccagatat ttctccaaca aagagagggc atcggcaggg aggacagtcc
     9061 taaggtattg catcaactcg ggcatagtct gtttgagcgg aaacagaccc ataaatgccg
     9121 tgagaaacaa gagcgccgga aagagggcca gcagaaagta gtacgccaac tgcgcggcgc
     9181 gcccgagaat gtcatcgtgc agactttccc gccacaaacg gcggaccaat gccttccacc
     9241 ctagaccgcc cagtttccag ggattcgagc cgggcgagga accggtgagc gaggtaggca
     9301 cggatctatc tccatttttg gtgtgggacg gccgccgtcg tccagcggga ctgcggatac
     9361 attttatgct gctccgtgaa caggtcatgg gagaagtgat gagtgtccag ttcttcctag
     9421 tagggaacta agatcaggtt gcctgagcag acggttgggc atcgttgctc cggctattcc
     9481 cgccgtcggt ccccttgttc gaagtggact gcgttcgtga ggaatactcg tccttcggcg
     9541 tgagtgccga gagagtgaca cccaacgtgc gagcgatttc aggcatggcc ttgcgagcca
     9601 attcaaacag cagtgtgcgc ccgacctgta gcaccatgtg cttggcttct tctgtatcag
     9661 ggccgaattc gcgcgacaga gaactccaga ggtttgagcc attgccgccg gtttctctga
     9721 acgaggattg cgattcgtga ggatagtagt catacacgcc ttccgactgc ctggactcgg
     9781 cgtttcgttg agccggagcc ggcatgaccc gggggccgcg cacacccggc gggtagtgtg
     9841 gatacactcc acggcgttga tgtctggtcc cctgttcaat caaaccgatg gcataccctg
     9901 cgcagagcgc accgcctaac atcagccagg gatgccggtc gattcgatgg accggatcca
     9961 caaccgcttt ggttttgttc acgaacgcac cgatgttgtc ggtggctcga ttcaacatgt
    10021 ctgaacacgt catcgtcacg tcctccactc ggtgtgtgat ctgttgctcc agcgcatccc
    10081 acttttcagc gatggcgatt ctcgtctgtg caatgtcttt caagtcctgg tcgatagcac
    10141 tcgttctttg atccatcgga tatcttcctt cagtgagtga aaggtgcgaa atggccagag
    10201 gcggagaccc gcgcctatcc tagccaccct atagagcagc ccgccggttc caagtgccga
    10261 gatgaacccc acaatgccga aacaagccca gagtggaagg tttgtatagg tcacgagtag
    10321 atggaccacc atcatcagca gaaagaggat cgccatcatt cccagaagaa gaccgattcc
    10381 gctttgtatc atgatcgagc tgagcttgtg ccgttcgtgc tgcatttcgt gacgtgccag
    10441 ttccatctcc tgtcgcagga ggcagcggac gtcggacatg agtcccgata cgagcgcgcc
    10501 gattgaaatt ggatgtggat tgtccatgtc cgaagccctt ccttcactat tcaggccgcg
    10561 tgcgagcgag caggtacccg cacccaagtc caacgaggag ggtttggagc gggtaacgcc
    10621 gcatgaggat ttcgagatct tcgacgatgc cgctcacccc ctgttgttgg agcacgcggg
    10681 aggtttgttt cacgcctcga gagacggcat ccgctgcggc accggctgca ccatcttgtg
    10741 gaagccgttc acgcagtcgg tcggccgagg attccagcgc ctcaccagcg cgtttggtcg
    10801 ttcgatcggc ggcagccatc gtgtcgctca cggcgtcatg aacggttgcc ttcatctgtt
    10861 cggcggtgtg ttcgagagtg ctcttctcct ggtccatcgg gactctcctt cattctcaat
    10921 tacggttgta gctccgtgtc atcggtatgc tcaaggtaac ggtctgcgtt ccgcgcatga
    10981 actgggcaaa gccctagtcg cgccggtgga ggcatgactc catccccgac atgtgccctc
    11041 agagggtggg ggaagtcgca cgaacctgtt tgggtttgac gaccggcggt cggtgcatct
    11101 gcagccggac gtcgggacgc acctgtttcg tcgatagtcg gagcgggacg ctacggcctt
    11161 gccgggcgga acgatgcagg gcttcgataa tttgcacatc gagcatgccc tcaagcccat
    11221 tcggttccgg ttggcgattg tcttgtacgc aggaggcaaa gtagatgagt tggggggcga
    11281 actgatcccc cggcttgaac gtacggacgc gtgtcttccc gtttacggtg atggaagctt
    11341 tcaagcttcc caggtattcg taggccggct ccacacgcac ccggcctttt gtgccgacga
    11401 tttcatacat ggcggcatcg gtcgcgccga agctgcaggt caatgtcgcc aggcgatcgt
    11461 gaggaaagcg aaggattgcg ccggccgatt cttcgacctt ttggaatcgg cggtcatggc
    11521 cgcgaccgac caaggccgtg acttccgtcg gttcatcgcg gaagaggtat cgtgccgcgt
    11581 tgatgcaata gatgccgata tcatacagcg gccccccgcc caacctctgg cttaaacgga
    11641 tgtcgccggg gcgaacctgt tgggtgaaga ctgaatgaaa gagcctcggc tcacctaagc
    11701 gcccggactg cagcagggca atggtctgaa gattacaggc ctcgaaatgc agacgatatg
    11761 cgaccatcag tttgacgcga taccgctcgg cggcccggac catcttccgg caatcggcgg
    11821 ccgtcacggc aaggggcttt tcacacagga tatgtatgcc ggcacgagct gcccgcacgg
    11881 cgtattcgcg atggagactg ttcggcaggg caatgtacac ggcatcaatc tcgccgcttc
    11941 gcaaacactg ttcgtagtct ttgtaggcat aggtgtgggc gactccgtat tgtcggccga
    12001 ggaggcgacg cttcaggggg tcgtccgata ccagcgcggt cagccgtgcg gaggattttg
    12061 catgcgcgaa ggcgggaagc acggcaacct gggaaatatg tccaagaccg acgaccgcaa
    12121 aacgcaaggg tgaaagactc attcagtgcc actccatttc tcccctcccg gggaagggga
    12181 tgtcatcatc cgtcgttcga cccgtcaccc tgtgtagcat tccggatgtg ctgctaccga
    12241 tgggcgtggt gctatccctt gttcaatagg ggaaagagat cgcgctcctc cgcgatgaaa
    12301 tgggcacgga cggtctgcat catgtcttca aagaatgcat ccaacgattc gtcgtcatcg
    12361 ttttcagcct gttgtaactc gtctatcatt gccttgatct cctcatgttc cacgaggagt
    12421 tgttccacga ggatacggcc ttcgttgccg tgatgccgaa cgtcctcagt caaccgctct
    12481 tccatgtgga aatggtctga taatcgttgc aggatctgct cgatgtacga ttgtcgggag
    12541 tctgccggtg tggcacggta cagctggaaa agggccagaa tatgacgatg gtcgtcgcgt
    12601 accagcttga ctgcgccgtc ggtgttcgaa atcgtcggtg tattcgccat ggggctgcct
    12661 cccaatgagt gacgcgtggt cgttcgtgag tctcaacgcc accgtatcag tcaatcgttc
    12721 gcgtgctcac tgggcaggac cctaatcatc gacgccacgg tgagcggcga cattcggagc
    12781 tcggagagga agcgtcgttg gacgcctcct ctccctgtgg tgcgagtcag cggctttccc
    12841 gcgggagcaa cgccctggtc ctttgtccct ccagtgtgac ccggccggtg gcgttggcgg
    12901 tcccttccag caaaatgatg accgtcaggc cctgcggcga agagcggacc tggtcgggca
    12961 gttgccgtgt cacaaggata tccatcgtga cgcctcccga ccggtgtgtt ccagtcagtg
    13021 tttgcttcaa tcgatcgact tgcgcagaga tgtcgaccgc catagcctgc cggagtttct
    13081 gattgagttc ccgatccacc agttgccaat agccgatacg aatggagtcg atggcggatt
    13141 tcgattcatt ggccatttgg aggtccggca cggagaggct ccgtcccccg tcatccaggc
    13201 gtggcgttcc ccaatagtag atcgtccctt tgagatcgcc gctgagttcg accgtcacca
    13261 ggacgcgccc actggcgtcc gcggcggaga cctgttcaat gacggccctg tctgaggccg
    13321 tggaatcgag caccaccgtc tggtggaaca gcttgctctg cagttggtgc gagagctgct
    13381 gataagaaaa tgggactcgg gccagcatcg tgtagggctt attaacagcc gacagagggc
    13441 ccgaattgac gagggcgact gtgggagcgg cttccgtaca ggtcggctca taagtgatga
    13501 agggcatttg ttttgccgag cccagaatca ccgtgtcctg agtcgtacct ttctgctgtc
    13561 cgagaatcat ttctctcgga ttgccgtaga ggcaggcatg ggtgttgagt gtcgccatgg
    13621 agagggacag gggaccgtcg agatcatccc agagccgttg caatggaatc gcaaacgcgt
    13681 cggcatggac ggcgtccgtc agggcgatct tgatctcagg cagtccgaac aagtcgggag
    13741 cctgatcggt caccgggacg ttgaacatgt tgcagcgcgc gtcgctctcc ggtttggttc
    13801 tcaccgcgag atgagtgggg tcgaatccga acgacaccga ttcgctttct tgcagcaggg
    13861 caagtttgcc cgtgacgtcc agcgtggcaa aaacaggatc caggcgacag gcccgtgaac
    13921 tgccgcgcga ttcgatctct cctttatatt cggcatgaat cgcgacgagg ccatcctgga
    13981 tatgcacctg tggattgctg ctcctgacaa aggtccaacg gaattcttga ccgagcggat
    14041 gccccgcctc cgtgatgcgt tccggaatgg catctcgaat ggccgtctga atcggcgtca
    14101 gatcggtttt aacctggaag ggcatctgcg agagcggagc ccgtgactcc tctttcgcca
    14161 tcgctgcgaa ggaaggaacc gcgctggtga tcggaggggg aaccggtttg gatgttgact
    14221 gcgggatgga atggctgcag gcattaccga gcagcaggac tgcgcagagc gccggtagaa
    14281 caggttgtgt atatcgaccc atattgaacc tccttaatcc atgcgtgacc gcagccaccc
    14341 aagaagcgga tgtggctagg cctgcgtgcg aggcgtacag agggctggca cttacaatgt
    14401 gccacgcgtt atctgtgcac tcccgctctt tcgaccaccg atgacaccac gaactgacga
    14461 gatcctgcct ggaccctcac gaggctcccg tcgattgtga gattgcatgc agccttcaga
    14521 ggtgagaggt agagacctgc ctgacgtctc cgcgaatccc cacatcctgt acgcgccagt
    14581 ggcttgcttc ttctcactgt ggttgcggtt gaatttgcag gttgttgttc acggtcctca
    14641 cgccggtcac tccacgtgtc agttccgcta cccgcatttt ctgttcggtg gatgtgacga
    14701 caccgttcag agtgaccacc ccgcgttccg tatccacgtc gatacgggaa aaattcgaca
    14761 agcgatcgga ggatagtttg gattgcaccg aagccgtgat ggtggcgtca tcgatggttt
    14821 gtcccgcggt cttgccggtc gtggactggc agccgaccag cgagagcatc gtcacagcaa
    14881 aaacgaaacg tgtcagatgt gcgatcatta tgggtactcc ttttcagaat ttcgtccagg
    14941 tgtatatccg tatgcgctca gaccatgtcg tacgaatact acgcgcgccg ggaggccgcc
    15001 tcgcccacgg ttttctcggc ggtctggacg gtctcatcca cgaattcccg ggcagcccgt
    15061 ccggcgtttc gcagcgtttg ctttccaccc tcgacatatt cctgcccgcg atccacggcg
    15121 tgatcccaag cggcccgacc ctgttcggta gcctcgtcca gttcatcctt ggccttggat
    15181 gcatagtcgc gcagggtgga acgaagctcc gtgcctgact ggggcgccaa aagtagtgcg
    15241 atccccgcac cgatgaatgc gcctgccata aatgccgacc agttggaaga ctcttctgta
    15301 tgcatctgct gacctcctta ggattgacga tgatgttgga ttgcctgatc tcaagctaag
    15361 caggggagca gcggtcaagc ggttcgtcgc ccgatccctc tacttgacta cacagtaaca
    15421 ggatgccgcg aacctcccac taggcatggc cctagttgag cggtgcgcag cgcttgaacg
    15481 cgtgaaattg cattgactat gcgtgacggc gagttatgcg ggcatgggcg gcatcgcgtg
    15541 atgagggagt ggaaataggc aattgagctg agctgttcgg taacggtatg tctgaaactc
    15601 aggcgcggaa aattcgtggc aatactgagc aggggacgag ggaaaatgaa acggtgtgga
    15661 ttcgacggtt tggcctatta gagcgcgcga tcagaatttt caaccggcat gtctgagaaa
    15721 cagactatca tgcatgatag gggtgttgca ccgagtgccc ctgaaccagg gactccgcga
    15781 gggttcgata caaagtgttc tagcccggac tattcatgaa tacctgctcc ggcgtccgat
    15841 cctctcgccc ggcgaggctg ttctcgttcg gccttctcga cggactgcca gtacgcctgc
    15901 gtcggcctca cgtgcgagca gcctcggcgg acttccgtct cgaacgcctt gcgacggcat
    15961 tcatgaataa tccgggcttg aatatcacga gcggaggtcg gcaaagtgcg gcaatagaga
    16021 tcggtccaga cacaactgaa gtgatcagta tcagccgatg cgattgcgcg tggtcggaac
    16081 gtggtggggg attgtggtgc gcccgacagg acttgaacct gtagccttct gatccgtagt
    16141 cagatgctct aaagtctaca cagttctatc ttgtttgata tacctttaca atacaatgag
    16201 atattgataa tctcagtcat atgctctgcc ctgaaatgcc ctccatttcc ctctctctgg
    16261 tctacaccag gtctacacga gagcttctga taggtcatct gatgcgagga cgccgatggt
    16321 gaaattgacg aagaaactga tcgatgcggc accctttcca aaacaggggc aaatgtttct
    16381 ccgtgatgct gaggtgcgag ggctcgcctt gcgaatcacg accggggcga aggcctttgt
    16441 gtttgaaaaa aagatcaacg gtcgggcgcg gcggttcacc cttggcccct atggtgcgct
    16501 gacagtagag gcggcgcgga aaatggccca agaaaagatc ggcgaggttg cgaaaggcga
    16561 cgatccggcc gaggcacgac ggcagcggcg acaagccacc acatggggcg acttagaaac
    16621 gctgtacctt gaacgtcatg ccatccataa aaaatccatg aaggatgatg tgagtctcct
    16681 gaatcggcac cttgcacagt ggcgcacctg gaggctgtcc gcgattacga aggcagaggt
    16741 ctgtagtaaa cacgccgaaa tcggcgcggc tggtcacagg atcgcggcga atcgaatgat
    16801 tgccctggtg cgcgtgatgt tcaaccttgc gaccgattgg ggcttctttg cgggagtcaa
    16861 tccggctgcg cgggtgaagc tgttcaagga agtgtcgcga gaacggttta tcaagcccga
    16921 tgaattgccg cgcctgtgga aagccttggg ggatgagtcg aacccctatg ttcgagttgc
    16981 gttcatggtg ggactgttga caggtgctag aaggaacgaa gtgctcgcga tgcagtggac
    17041 ggacctggac gtgtcgcaag gtttgtgggt gattccatcg accaaggcag gccgaatgca
    17101 caccattccg ctcccgcgcc cggcgcttga ggcgttgata ggccttccga ggttggaggg
    17161 caacccctac gtattttgcg ggcgatgggg taggggccat ctggtgaacg tcgcgaagcc
    17221 atggcgacgg attcgccagc aggcaggcct taacgatgta cgcattcatg atcttcggcg
    17281 aacgctcggc agttggcttg ttgcggcagg cgccagtttg cccttgatcg gaaaagcgct
    17341 caaccatagc aatgtttcaa cgacgcaagt ctacgctcgg ctgcaactgg atccagtgcg
    17401 ggtagcgcta gaggacaatg cggcgaggat gttgacgata gtgaacgagg cgcacgaggt
    17461 ggagcatgcc gaagggtaag ggcaagaatg ggccgaagaa atcgccaagc aagccgactc
    17521 gtcggcagag ttcagaatct ctatcggcgc aaaatgctaa agccgtagcc aaggaattgg
    17581 aagaaaactt catgatgtca tgcatggcag cggaggcctt gcctgtcctt cttgctccgc
    17641 tgattgaccc ctacgcgacc atgctgttgg agcttattga acagcgacaa cctaaaagca
    17701 cttccgaccc attacttcag ctccatgtat tgcataggcg ggtgcttcag ataccagacg
    17761 agactcaccc gatcgacgca gatgcagccc tattgcttgc aggcgaagtg gggattcaag
    17821 ctccagcatg ggcggtagac ttggcaagga aagtgcgcct tgaaaagtac tcaagcaggg
    17881 acatggatct taataaaaca cttggattca cagcagaggg caaaggccgc actgcggaga
    17941 tagaacgacg cgcacgtgtg gcgattgtct tccgatccat gctcaatata ttggggcttc
    18001 gtttgatcgg tgtctcaatg gaagatgctt gtatgcttga ggccggacgc atgcagaggc
    18061 ttgggaagag ttggaacaaa tcagcctacg acatggtgcc tgaccttggc tcacatgtta
    18121 gaaatcccga gaaacgcgat agcgagttac gagataagag atcgcgatat gccgagaaac
    18181 tacgtcaaga atggtacgcc tggaaatctc cgatgaaaga tagcgactcg ccggaatcta
    18241 tcgccctaaa gactgatatc gaggccgaca aggccaagtt cctggcacag tttccgcctc
    18301 tctaacaatt aagtataccc cctagtatgt gaggacctat tataacggga ctcctcacac
    18361 ccctcttatt cccctatcat gcccctcgta gcgcttattc actacggagg gacgtatggg
    18421 atcaaacaaa caactgcaag acgatgaacc gctgcgcgat gatgcgtggc ttgctcagta
    18481 ccttcactgc gagcgcaaaa ccgctcaggc gtggcgcact cgcgggaacg gcccccccta
    18541 cgtaaaatta aattcgatgg ttagataccg caagagcgac gtagaccgct ggctagaaag
    18601 tcgcttggtt tcctccacca gtgaggccgc caagtgactg cggggccgct tcatcctgag
    18661 gcatggtctg attgccaaaa gagcggcctg actactgaga ctctcaatcg ctgcgagatc
    18721 gagtccgtaa gtccacatcg cttaccggct tgcggcgttc ctgacgtaac gcatgctacg
    18781 gagttcccat atcactccct tgagggcacg ccggcaggct tatcccgctg gaagctattt
    18841 tatgcgggac acactgaaga tcggccgaaa tactggcaac cgaaaggcac tgatccgttg
    18901 ctgtacctgt cgcgccttat cgaatggcgc agcatcgcaa acgattacca aaagctgatc
    18961 actatcacag agggcgaaaa gaaaagcctc gcggcctgtc agcttggatt gccatgtgtc
    19021 ggcgtagctg gtgtgtggaa ttggcgtgca aggctggatg acggcgaacg gatggcatta
    19081 ccaggcttgg accagttcac ctggaagggt cgcaccgtcg aactgattcc agactcggac
    19141 gcatggcggc ccaataaaga gggcgatatt ttggccggct tctatgcgct cggccatgaa
    19201 ctcaagggcc gcggcgcctt cgtctctttc gttgaactcc ctgagtataa cggtgttaag
    19261 tgcggcctcg atgatttcat cgtgcgggcc ggagccttcc ccctcgaaac atttcaaggc
    19321 tgtaaacgct acgctttgga tcatccacga ttcaaagcct tgtcagcgtg gcatcaaaag
    19381 tgggagcggc gacaacgggg cggaaatcaa ggcctcgtca agaccattgc ggatgaaatt
    19441 ttaaaaacgg agcattttgc caaagacgcg ggcggcgaac tatttgcgta tgaggctgga
    19501 gtttatcgac cacgaggggg cgagcgcatt gctcagtgcg tcaagcgctt gctcgacatg
    19561 agtgccgaca gcgccagatg gagcagccac ttggctcgag aagtcgtgga gtacgttcgt
    19621 atcgatgcgc cttccctgtt ggagcggcca aggccggata cgctgaatct tcgcaacgga
    19681 ctgctggata ttctctccgg gacactacgc ccgcatgagc cggggcactt atcctccatt
    19741 caactgcctg tagcgttcga tccgtcagcg atgtgtcctc aatgggaagc attcattact
    19801 cgcgtactgc cggaggactg ccgaaacatc atctgcgaga tcatggcctc atccatgcgc
    19861 ggggatatct cagaccagaa ggctatcttg ctccaaggtt ccggcgaaaa tgggaaaagc
    19921 acgttgcttg atgcagtaat cgcttttctc ggtcgcgaga atgtttcaag cctcgcgcta
    19981 caacgattgg aggttgataa atttgcggct gttcgattgc tcggcaaact cgcaaatgtg
    20041 tgtgccgatc tccctgccga tcatctcgca tcaacctcga catttaaggc cttaaccggt
    20101 ggagatatcc ttaccggcga acggaagttc caggggtctt tcgagttcgc ttcattcgcc
    20161 cggctcatct tttcgacgaa tcactaccca caaagcaagg atgcatcaca cgcctttttt
    20221 aggcgatggc ttgtggtgcc gtttgatgcg gtgattgacc cgcgtgagcg gataccaaac
    20281 ctcgcggcaa agctcgccga cgctcaggaa ttatctggaa tactcaatcg agtgcttgcc
    20341 gtactccctg gcatgattca gcggggcgga ttttcgcaga gtgaaaccac tcaggcggcc
    20401 atgatggaat ttcgcgaaat gaccgatccc ctcgcggcat ggcttgaccg ctgcactgtg
    20461 ctgagtccgg aacagctggt atcccgaaaa gacttgctga tctcctataa cgcccaggct
    20521 gaagcatccg gacgaccggc gctgacctca aaggcctttt gccaagctat ccggcgactc
    20581 aggccgacga taagtgaggc acagcggacg gtatgcggcg ctatgcaatg ggtattcctc
    20641 gggcttgggt tggcggcttc accaccttca ccctcacaca attcacacca ttcacacgat
    20701 tcttcccaga taagccttga ggtggagaca gataaaggag aggtaaataa aaacttaaaa
    20761 agggggaatg gtgtgaatca tgtgagtcct gtgaacgagg cggttgcgac catcattccg
    20821 tgttttaact gccgcggaga acgtttctgg gaatcgattc acggggcaag agcttgtgca
    20881 acatgtcatc cgcccgtgaa tcctgggctt gttcgcgaat ggattgagaa tgcaccggtt
    20941 aaccagtaat ggaaggaata tctatgctcg ttgaagcaga cagcagaaaa catcaagtca
    21001 tccttcgcag tgactcagga tccgtaatca cgctttcatg ggccgcggct gcgaacttaa
    21061 gcagcttgtt aaatgaagcc tcacggcaag ccgaaccgcc gacacgtcca ggccggagtt
    21121 attgcccagc tgtggagcga tcgcgataag ccatgacccc tcgagaagtc gttcaagcca
    21181 ttgcggccgt gatggagcgg caagaggcca ttcaaaacac acagctgtta atactgcgcg
    21241 agcttcgcat catgcggtgt ggggctggtc gcgtcgatga ggactcactc gaagcgctct
    21301 tgggatatct gtatgacgaa ttccacaacg acacatggac ttcaagagtc gccttcgatc
    21361 aggcagcaga gaacagagca ctctatgccg cgttggtccg gtgtctcggt gaggaaggaa
    21421 ccccgaacgg ccttggcaag cttctgaaga acaaccttgg caagaccggt gactattctc
    21481 ttgagtgcgt caacaagcac agcaatgctg gcgcagtctt ccgagtgacg gtctccgtca
    21541 catcgtcacc tttgaagcct tctatgatgg aggaacaaag gagcacatca tgacgcacga
    21601 aacgaccctc agcgatttgc atcattcctc gattccttgt cccgatgctt tcctctcagc
    21661 caaggaaagg cggaagtatt ccatccttaa gcctctgcgg gcgttggtcc ttggggcgca
    21721 gaatcctcat tttttcgaag aggcgtcact tgagctcgag gcttcgcgtg aagtggctag
    21781 acggcacagc cgtgaggcaa gcacctcatt cttcgtgcct gctgaagtct tgcagcggcc
    21841 gattagccag gaagcctact ctcgcgcgat ggcaaccact cccggttcaa agggggggta
    21901 tctcgtcaat accgccaaca tgggctttat cgatgttttg cggaatcgat cggtatccat
    21961 ggcgatgggc gctcgcgtct tgtctgggtt ggttggcaat gtgagcttca cccgtcagac
    22021 cgggaaagcc acggtaacct ggcaggccgg cgaaggggtc agcgcctcgg cttcggatca
    22081 ggcattagga cagattacga tgagtcctaa attctgcatt tcgattacgg atgtgtcgct
    22141 ccaattgctc agtcagacat ctccggccgc cgaatccttc atcatggccg acttggcccg
    22201 cgatgtggcg atcgacggtg tagacgccgc ggtgatcaac gggactggag gggcccagcc
    22261 tttgggcgtc aagaatactc cgggcgtcaa tacgggtcag gatgcgtctt ctgcgaccta
    22321 tgccaagatc ttggccttcc ctacggttgc cggaacagcc aatgcgctgc gcggtaaccc
    22381 tggctttgtg acgaatacgg ttggcgctgg gaagctcatg ggcgtgcagc ggttcagtaa
    22441 taccgatagt ccaatctggg aaggcaatat catgaacggc gagctagtcg gctttcctgc
    22501 catgtcttcc gaacagttag cctccgggaa cctcatcttc ggatcctggg aggaaatttt
    22561 aatcggtgaa tggggagttc tggaactcgc gaccgatcgc ggcggagatc gattcaataa
    22621 agcgcaagtc ggcattcggg ccatgtggat ggtagacgtg gcgatcagat acccacaggc
    22681 ctttgtggtg ggaacgaacc tgtcatgagc gcgctgtgct tcctctgtga gcattgtaag
    22741 ggcaacgctc aacgaatcat ggctactcgt tcgagcaaac cacagtcatt ttgttgctct
    22801 cacaacaagg tacgggtgca ggtgcatgct cgtggctgtc ggtgggagtt cgaggaggga
    22861 ctgattcaaa ccagtcctat tcacgtgatg ccgaaacggt ccattcatga gtaagagact
    22921 atccggcctg tcctcgattc ctggcatgga catcgggaat cggccgcatg gtcactctgg
    22981 gcggaggccc ccgttgatcc ttagttggcg gtccggcctt cgcctgcaga ttcgcaatgt
    23041 gcccatgagg agtaacggat ggtaggccca gtagcaactg actctgtact agtgaaagcc
    23101 ctgaaaccgt ttcgccatga gcatcggacc tggcgcgcgg gtcgcgtctt ggccgtccat
    23161 caatctgtgg caacatggct actcgaaaca gggcgagcaa cattatgcga aatccagata
    23221 ggcggaaaca ggcagacctt atgacaaaga gaggtaaaac tgcgccgggc ttttgcgtga
    23281 cgttgaggct cggcgaatcg gtagctgata cagggaacgg cagacctcac ggctctgctt
    23341 atcgctgggt tagcagcctg ccgcatgggt gggggccgga ttccggcctg ccacctgtgg
    23401 gtttctacaa tcgttcaagc catgccggaa gtgttttgat gggagcatgc ccggcagtct
    23461 gccatccgcg gtcgccatac tgcgagattg gatcatgtcc ttctgcccgg tttgctcgcg
    23521 ttgcaggtcc gggcggtcgc cacatgtgcg gccgtgccgg gtctggcgtc tcagccaagg
    23581 aaagccttga tcgttcagcc tgtgggcggg acggcagcgg agcgggcatt cttgggaaca
    23641 ttttgggtcc tggaaaagtt cgaacgctcg cgcgggtggg aaggcacacg aacgtgacag
    23701 gcgcagacac ttttataccc ttattgctgt gaagcaggca cctgaatgaa acaatacctt
    23761 gttccactgc gtattccgac ccaagccggc cgctgattcc gacgcaagtc ggccaccc
//
LOCUS       DAORRQ010000060        23208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_132_length_23208_cov_153.757267, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000060 DAORRQ010000000
VERSION     DAORRQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132_length_23208_cov_153.757267"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1080
                     /locus_tag="PK224_16015"
     CDS             145..1080
                     /locus_tag="PK224_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRB17300.1"
                     /translation="MEGAPETVKQAERVLNELASLTNEGYDLRSDDVTHALTALRHNH
                     DASLKELLFSASPIVTRKRFIVPKTPTQKYYLDAIEKHDIVIGIGPAGTGKTYLAMAM
                     AVSALMKKDVSRIILARPAVEAGEKLGYLPGDMYAKVNPYLRPLYDALFDMMDMERAN
                     RLIERGDIEIAPLAFMRGRTLNDSFVILDEAQNATAEQMKMFLTRLGFHSKAVVTGDI
                     TQIDLPSDRVSGLIEVRDILQDIAGIEFVYFDERDVVRHRLVQEIIKAYDRHTASPAP
                     PAQPRKGEAQTKGHRSDSKPTRPAAPSTGSWGQSH"
     gene            1080..1550
                     /gene="ybeY"
                     /locus_tag="PK224_16020"
     CDS             1080..1550
                     /gene="ybeY"
                     /locus_tag="PK224_16020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRB17301.1"
                     /translation="MPVVIGMRLTRWPLRLGALNQLAMHVLQAVGEAETLLSLEIVGD
                     VRMRRLNRIFRHRDKTTDVLAFATREGPGPPSSLLGDVVISLPQAIRQACRHEQGVDH
                     ELAVLLIHGILHLCGYDHERSEAEAQRMSRREKAVLRAVAPVPRLLVSRGSDQV"
     gene            1639..2553
                     /gene="ftsY"
                     /locus_tag="PK224_16025"
     CDS             1639..2553
                     /gene="ftsY"
                     /locus_tag="PK224_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRB17302.1"
                     /translation="MGWLQKLSAGLSKTRDAVTGQLDRLLGRAADPALLDELEVALIS
                     ADLGMPVVERVMGQLRAQMRGGNFSSADKVRDLLRQSVLDILLPTQSASMEQLVAQGP
                     RPFVILAVGVNGVGKTTTVAKLTQRFRQQGKKPLLVAGDTFRAAAIDQLQVWADRIEV
                     DVIRHRPGADPAAVAYDGMTAAKARGSDVVLIDTAGRLHTKTNLMDELRKIKRVVAQE
                     CQGAPHEVLLVLDATVGQNAIAQARQFHEAVGVTGIALTKLDGTARGGIVVAIADTFK
                     IPVRLIGVGEAVDDLQDFDAKAFVDALF"
     gene            2757..3545
                     /locus_tag="PK224_16030"
     CDS             2757..3545
                     /locus_tag="PK224_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17303.1"
                     /translation="MMSTLLVIDDDRLHCDLLQMALARHGYQVSIATSGREGVVLFQQ
                     LRPLVTLLDLRMPEMDGLAVLKEIRTHDPRAGVIMLGGGATEELENQARELRVTDFLR
                     KGLSLDVLIGAVHRVAQQARRQASSEQSHAGGEIDQLPDEQILVVDDDVMARDLLVRF
                     LSLRGYHVRAARDGREALRLIGESAPDLLILDLAMPEMNGVEVLRTLTARDYAGRTII
                     LGGHQNDPLLAEAWALGPQEVLDKPIDLERALMAIQLVMVCREC"
     gene            3588..5768
                     /locus_tag="PK224_16035"
     CDS             3588..5768
                     /locus_tag="PK224_16035"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRB17304.1"
                     /translation="MYRLIRFSLLTICLLPAPAVADSVAPPPTIEHHQLALELSPDSH
                     RLIATDRVTVAGGFPGQELAFSLAPTLLLERVEDLTHCQDPCEFAPSVPFARDRSSSQ
                     AESQRILLKQPPANQAGGSIRLKFVYHGVIDDPPRDPRHLRFVTPSETAGHIGPEGVY
                     LSSESQWYPDLDDSLATYDVRITLPEGWAAVTQGTAQADASRWIVSTKSEALTVVANR
                     FVVKTRTWKAQSGQAIQLATYLFPDDAALADEYLDASARYLDAYIPLLGAYPFSQFAV
                     VENFFSSGLGMPSFTLLGSGVIKRHYTQPYALGHEIVHSWIGNGVFNRVSRGNWVEGL
                     TTYLSNYYYHELTGDEAQAREQRRLMLLGYSVYVRPGEGYPLRRFAQKSDEKDNAIGY
                     QKSAMVFHALRQEVGDAAFWRALKRIPEQYLGTVADWSDLERTFQEEAGRDLRWFFAQ
                     WVERAGVPELALSGLRLQTVSGAFGHANAVEAAIHIAQSDEPYRAPVELEFVLSGERT
                     HRVRVQLSAAQEDVVVPLAEPPSAVRLDPDYHLFRRVARSEMAPVLNLYVTDGQRSVV
                     LAQGSPAQPGPFGDILQRIVAQESAKPDAVRTIVLQPGEGNRSVPSGSLLVLGDPREN
                     PIAAAAVRSCGDHVRFLDGGFSVAGKMYEGAAMALLVSCRREEHPGSVVTLLYGVTPQ
                     ALGRVARLLFFYGWQSYVVFQEGAVVARGDWEDRMNTEVRIETR"
     gene            5755..6888
                     /locus_tag="PK224_16040"
     CDS             5755..6888
                     /locus_tag="PK224_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17305.1"
                     /translation="MKHVKTMMIGMVGLALVATSTVLAGAAEPKSAQAPANASASAER
                     HLTNIRQLTFGRQNAEAYFSFSGNKLIFQSTNNWMKDTFAAAMHPSDIPLGCYQMYVM
                     DLGSEKIRMVSTGAGTTTCGYFFPGDRRVLYSSTHLRGPNCPPKPKRDGGAYRWALDD
                     YDLFSVRIDGLDPQRLTNTPGYDAEATVSPNGKTIVWTSMRDGDLDIYAMDLDGTHPR
                     RLTQEIGYDGGAFFSPDSKRIVYRAQHPSNQEELDQYKALLAQNLVEPGRLEIFIMNA
                     DGSNKQQVTNNGASNFSPFFHPDGHRVIFASNVDTRNAARRPEFHLYLVNEDGSGQER
                     LTFEGQFNSFPMFSPDGKTLVWVSDRHAKEPGEFNVFLADWVP"
     gene            6885..7970
                     /locus_tag="PK224_16045"
     CDS             6885..7970
                     /locus_tag="PK224_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRB17306.1"
                     /translation="MIEPGDGQAASQPAQPEPPPACPSIPAVLLSVIVLFGSFAALFY
                     IDIPILWFLRSHNLSALQSLGDLGEKLGNGGTLVTISLVLLGAGYMLKRQALTRVALD
                     SLLAHGAVAILVNGLKHIIGRPRPRLTHSGGWQWWPSLDSGLDSFPSGHTSATVAVAT
                     VLARALPRYRWVPFALAAWVAASRVWRGSHFPGDVVAGMVLGFVVGSIFNGPLRWWGR
                     SCGQALIRITPVVLSLTGLFWVLTHRIVDPMTDHILLASGAMLVAGGMALRMAGRRNP
                     AGDGSVTRVAAAESLVGLGLGVVTGAPVVIGLAGLLCFARWNGGVACADPCSVAPPSS
                     SRHILYLAGVIAAVVVIQSLKGLVPLQ"
     gene            complement(7979..9766)
                     /locus_tag="PK224_16050"
     CDS             complement(7979..9766)
                     /locus_tag="PK224_16050"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRB17307.1"
                     /translation="MNGQHPSSQTPAQADRSIQPLALILLLALAAVLFFVGLGSLGLT
                     DRDEGRNAEAGREMYETGNYISPTFNYEPRFAKPVFVYWLMSLSYHVFGVSEFAARFP
                     SAIFGVGLILLQYLFLTRCRGPVVGLFGAAMLLLNLEIIGLSRMALTDSVLIFFTTLS
                     LYGFWLGLYGEGRERHYLWFFYIGMALATLTKGPIGFLIPMLAVGLHLWLTRSWGLFW
                     RHGFPIPGLLLFLLLALPWYLIMLNIHGQRYTTSAQGDTIGRFFGTMEGHGGTLVFYL
                     PVFLLGFFPWSGLLPFAWYQAYRSWRDSKRSGALPPSQTSVLDVHPSPHALEWFTAAW
                     VLGGLVFFSLSSTRLPHYIGPLFPAAAILTASYWNRCVTDQATPGLRAAIHTITAVGC
                     ILALAFASLPPLYAKFAGKLIDEFPLAGQVTLGPGPYTVASIFLVGMGLIAYFGFSET
                     RRPAVFWVAGGSLALVVLATTQLIFPLVHHFVIEPPQQLAEVAGVNLGANDRLILYGQ
                     PRPSLVFYAKRKAIVAPKNEEANIKPYLTQPGRTMILLPAALRNRLPFETMDYPVLLE
                     RYGYILLANQSLIHVPEEAEQPPVRIPGH"
     gene            complement(9778..10077)
                     /locus_tag="PK224_16055"
     CDS             complement(9778..10077)
                     /locus_tag="PK224_16055"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HRB17308.1"
                     /translation="MTLDTIWLIIGFLGQGIFFMRWVVQWIASERHAESRVPTAFWYM
                     SMIGGLITLAYAIYRQDPVFIAGQSIGSIVYLRNLMLIHRPNQADSGTASPATKS"
     gene            complement(10074..10850)
                     /locus_tag="PK224_16060"
     CDS             complement(10074..10850)
                     /locus_tag="PK224_16060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB17309.1"
                     /translation="MTVVTRPWASVVIPIKDERENLVPLTEQLVTVLDGRGESRSAPF
                     ELIFIDDGSSDGSSDVLDGLAARYPTVKVFHFDRNYGQSAAFDAGFKQSTGDLVMTID
                     GDLQNDPADIATLLPHIKTFDLVCGWRKDRHDNLTRKISSRIANSVRSAVTGDRVHDT
                     GCSLKLFRRPVVEKMQLFEGMHRFFPALALMHGFTVTEVPVRHYPRTRGTSKYGVGNR
                     LFKGLYDLVAVRWMQHRCLRYQYRAAATTSSPSASAPTRL"
     gene            complement(10974..12668)
                     /locus_tag="PK224_16065"
     CDS             complement(10974..12668)
                     /locus_tag="PK224_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB17310.1"
                     /translation="MNQRPTTTPTYPQYQLTEILRDIFAHLGMLLKLERSILGIIASY
                     AVAIGFFLLCVPIAVQELVSTFSFAMEPRMIFTLALFVASSLTGVAAFRVLQARAVET
                     FQQRIYTRIAIGFTRLLPRLRDDTFATPQAYRFMEADLLTRALVAMVADLFNVAVVGT
                     IGVTMLILFHPFFVLYILVLILGFVGLLTLFGRGGFFITLEMSRLHYDIFGWIQNIAH
                     NLPHLRAAGDSPYLLERTDTLTRLYARIRQRRSDTLTGRQYKAAALWQVAGHSGLLIT
                     AGLLVADGQLTVGQFAAAEVLVGNLLINMDTLARRMVAMFFTFVSCREMAAVFSLPTE
                     EDDAKEDVPAAQFGAAGIHLTCRNLTYTGPDGTPIFENVSLDVAPGEKVAILCNTNHA
                     KTALAKVLAGLHPPASGFVRYNDMNLIEVKRDSISRVRGLVLDSHPTLLDGTLEDNIT
                     LGRPTIDYQDLQWALRFVELDHDIDALPQGLNTRVSSLKTNLSMSQILRLLVARAIVI
                     RPQLLIFDGTLHNMLPATREVLLRRLCAKDEPWSVIFLSNDPNFSAFVDRRISLDS"
     regulatory      complement(12689..12764)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(12923..13654)
                     /locus_tag="PK224_16070"
     CDS             complement(12923..13654)
                     /locus_tag="PK224_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17311.1"
                     /translation="MQWDILATILLTATIQSLFGVGVLLFGTPILLVLGYDFITALTI
                     LLPISLTINLLQVSKDYRHVQVPFYRQILTLSIPCVVLCLLLVTTLKLNIGSIVGLFL
                     IVAALKSVYVPFNRLIESLVRYERTYFIVMGVVHGLTNLGGSLLTAIIHNKQYDKDQT
                     RATTALSYGTFALFQLVTLGISLGPSAIATSDTALYMAVGVSMFLATELLLYSKMSTE
                     RYRTTFAAFLFLSGTALIFKSLFLR"
     gene            complement(13777..14352)
                     /locus_tag="PK224_16075"
     CDS             complement(13777..14352)
                     /locus_tag="PK224_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17312.1"
                     /translation="MRQLLWFCCPIFLLSGSLLWAEDLPLTRNVPIPDFQNRTESAKP
                     YNFDAPPEGMFRHIVLAEGFDEELGFQRTHEIVPVRPTEQFAAGSRPVFIVFELHQHY
                     QSFQVFGRCYPEEVTGLDGRTVVAEDAMYIALEDQTGYLKLFPTESGWKPGRYKVEIH
                     AGEQINDMSLMGTMRFTITASSDLGAPARSR"
     gene            14540..16024
                     /locus_tag="PK224_16080"
     CDS             14540..16024
                     /locus_tag="PK224_16080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRB17313.1"
                     /translation="MEQHLMTQLEDPDALPQPLGEYKPVDYWQAHINTLFYQLRGDQQ
                     RSFYQTFTSADYRLAHALAADYFEQVTKRDKKVAANRMTSNGPTATPSTDATPQAQLT
                     VMEWGPGNGNLAACFLSQLQRLDKNGQVYPRVRYLLVDSQAHALERARAHPDLAPHLA
                     KVESLCAEVENLATIADGTVDRILCNELWNELATKLMVKKGGEFEEEHLRPNLNERKA
                     AAIADWSGFVRAFEAKDIERLKQFPPFLDDLIWEREYHKVDWKDVPYRKTITEFMKAI
                     DDEVLVPVNLGAFASLKEAKRVLAQDAVGFSSFDAGTADMEVLNDPDKPCYGQFGGQY
                     SFMVNLALIQAVAKHLGLNAVTIETQREFVGSRLGTNVMTLMDLLACHPMVGSKVQPW
                     ELDRLTVKTIRTLNETYESPYQRKIEFPLRSEMPAEERDAAQGILLSLKPNGIPDTIA
                     YVTEEELSQAQPELENLGYEREAVLMALGAPPSPVEYYHFACRP"
     gene            16298..16582
                     /gene="tatA"
                     /locus_tag="PK224_16085"
     CDS             16298..16582
                     /gene="tatA"
                     /locus_tag="PK224_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB17314.1"
                     /translation="MFGSFGWMELLLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSV
                     HEADAIDVTATEAEPAPTQPSAQIQQPGQPATPPPAQQAGAAPPPRTTQG"
     gene            16678..16977
                     /locus_tag="PK224_16090"
     CDS             16678..16977
                     /locus_tag="PK224_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRB17315.1"
                     /translation="MFGLGAGEILIILVIAFLLFGPKQLPEIGRQVGKAVKGFKETAD
                     DLKKTVEPELNMIQQEMKMVEQDFESSMKEAEEQINHATSGVEHGAEESGLPKQA"
     gene            17229..18905
                     /locus_tag="PK224_16095"
     CDS             17229..18905
                     /locus_tag="PK224_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRB17316.1"
                     /translation="MKRIHGRTRWGRLAAIFGAAAVTALQCLTVPAFAGFELPPGERI
                     TNLPAIPRNMPQKEAYEIYDPVIGRNFDIKNLWMRADLRVRPEMRNNTCFGGGMGTGG
                     ACNTFGGANARGVAPNVNNNGNKGNDFFVQQWMRLGIGYDLSPDVNFYVEIIDSATWG
                     GNGSAVNAGNGGDPLTHNGASAGGAGNGGRLGVRAAYMLIRNLADVQGLSVKIGRQYV
                     VFGNHSLFGHFDWANTGYSHDGVMFAYQTKNWDSYFGWFRNSESDLGQAAPVGSGAAN
                     IAGTGGQDAQRDADMFIFYNQIKMVPGMVIEPFYVLYQNRYGSADNATQGLGTAKHSN
                     QTRHMIGNRIEMRKGGFDFSNEIAYQFGQMGQAGACQGEQKCLHINAWATRNWIGYTF
                     YDTAWKTRIAFNLDYASGDSRNNTCGATAGSCKTANTFENFFPTNHIHMGYMDVQAWK
                     NMLSPSVNLQARPSARDHIELWYTNLNLANSKDCWYRGAQGCYVFSNANNTKTHIGDE
                     IDVSYTRMFADGKVALQTTYGTIFSGGYLTSTLNQTQNQHWAYVSLWMNF"
     gene            18987..19418
                     /locus_tag="PK224_16100"
     CDS             18987..19418
                     /locus_tag="PK224_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17317.1"
                     /translation="MMKKLMFVTLGIAAMAIGSQPITAQAQVADAIQAVNDAIVELTD
                     APGLGKRTTVDLAKRWGADRSVVDSATAKLQDGFSKAQSGGAGADAMRQLKLAVDYGK
                     ARLHKEARLSAQGALRHLCVANQDQGPGCDTVPKFGSYTAP"
     gene            19636..21498
                     /locus_tag="PK224_16105"
     CDS             19636..21498
                     /locus_tag="PK224_16105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRB17318.1"
                     /translation="MDDLSTQLSRTFGFSAFRAGQREVMEAVLARRDAMAVMPTGQGK
                     SLCYQLPATLLSGVTLVISPLIALMQDQVTSLTARGIAAAAFHSGLSEQERDRVVLDL
                     KLRRLQLLYLAPERMQHERFLRLLRSLWVSLLVVDEAHCISQWGHDFRPDYLNIGRLR
                     RELENPPCLALTATATARVQADLCDRLSLQDPLRLVTGFRRSNLALSVRLCRSRQEKL
                     ATLDRVVREYETGTILIYCATRRAVEEVAACLGQSQSSVGYYHAGLSDEERRKVHDEF
                     RVGTVRILAATNAFGMGIDKSDVRLVVHFDIPGSLEAYYQEVGRAGRDGRPAACLLLF
                     HERDVATQEYFIQQASKESGSSARADRMKTLLQDLLEYVSVPTCRQLAILDYFSDEAE
                     RALGPCGLCDRCVVTPQPSAHVVGDEATCAKAVLAAVSWCMGRFGVSRIVDMLRGSRS
                     KALLTYGAEECPGYGGYHAWSKTALTHLVKALIDAGYLQVEGLEYPTLDLTRKGQEVL
                     KGISPLVLSEQAEDPAASTLKPQGSGRGSSMAPLHTASVDPQLFERLRQLRRELAEEE
                     GVAPFVIFHDKTLRNIAGYKPVTLAALLEIPGIGEVKVERYGRRVLGVVNEACD"
     gene            complement(21577..22197)
                     /gene="thrH"
                     /locus_tag="PK224_16110"
     CDS             complement(21577..22197)
                     /gene="thrH"
                     /locus_tag="PK224_16110"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HRB17319.1"
                     /translation="MQNPVIVCLDLEGVLVPEIWVNFAIKTGIEELKITTREMPDYDA
                     LMTRRLSILDQHGLTLADIQSVIDAMGPMEGAAEFIAWLRERTQVIILSDTFYEFALP
                     LMRQLGYPTIFCNQLEIGPTGRIVHYKLRQPNQKKHAVAALKNLNFRVMAAGDAYNDT
                     AMLGEAHAGFFFRPPDHLPKEFPQFPVTKTYAELQEQFRKAGNLRE"
     gene            complement(22281..23078)
                     /locus_tag="PK224_16115"
     CDS             complement(22281..23078)
                     /locus_tag="PK224_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRB17320.1"
                     /translation="MSNHTAVEHFEISLQTPSGEISTAVGVPTSFVPITAILPLMRSL
                     GEEAQVLEQRRLLQAGERVSCEKGCAACCRMLVPISVPEAFALTNTIDRLDQNERNRL
                     LSKLDLAQQQLARAGILKQLSSLADSSEPLSDEAIEPLNRAYYALRMPCPFLDNETCS
                     IYADRPAACRELAVTSPATECQDMTTPTVQPVPVAVRLSTALSLLWADLTGTAPRLIP
                     LPLAVDWARRHKEEQTNQWAGTELFEKAMDKIWRYLSQEKARRSNAG"
BASE COUNT         4655 a         6343 c         7455 g         4755 t
ORIGIN      
        1 ggagttttcg acaccggcgt gcgcaagatc aaactacggg aaggcacgaa taccgcagcc
       61 ctgttcgggc accatgatcg acacctcaag ctgattgagg aagagttcgg tgtgcgatgc
      121 tcggcgcggg gtgaagaagt gacggtggag ggtgcgcctg agaccgtcaa gcaggcggaa
      181 cgggttctca atgagctcgc ttcgttgacg aatgaagggt atgacctgcg gtccgatgat
      241 gtgacgcatg cgctgaccgc tctccgccac aaccacgacg cttctctcaa agaactgctt
      301 ttcagcgctt cccccatcgt cactcggaag cgattcatcg ttccgaagac tccgactcag
      361 aaatactatc tggacgcgat cgaaaaacac gacatcgtga tcggtatcgg tcctgcgggc
      421 acgggcaaga cctatttggc catggcgatg gcggtcagtg cgctgatgaa aaaagatgtc
      481 agccgcatca tcctcgcaag gccggccgtg gaggccggcg agaagctggg gtatttgccc
      541 ggcgacatgt atgccaaggt caatccctac cttcggccgc tctacgatgc cctgttcgat
      601 atgatggata tggaacgggc caatcgtttg attgagcgag gggatatcga aattgcgccc
      661 cttgcgttca tgcgcggccg taccctgaac gattcatttg tgatcctgga cgaagcgcaa
      721 aacgcgactg ccgaacagat gaagatgttc ctcacacggt taggcttcca ttcgaaggcc
      781 gtcgtgacgg gggacattac gcagatcgac ctgccgtcgg accgggtgtc cggcctcatc
      841 gaggtgcgcg acattcttca ggacattgcc gggatcgagt tcgtctactt cgatgagcga
      901 gatgtcgtgc ggcatcgcct tgtccaggag atcatcaagg cctacgatcg tcatacggcc
      961 agtcctgcgc ctccggctca gccacggaaa ggtgaggctc agacgaaggg ccatcgttcg
     1021 gattctaagc ccacccgtcc cgcggctcct tccacaggtt cctggggcca gtctcattaa
     1081 tgccggttgt gatcggcatg cggctgacgc ggtggcccct tcgcctcggc gccctcaatc
     1141 aactcgccat gcatgtcttg caagcagtcg gcgaagccga aacgctgttg agtcttgaga
     1201 tcgtcggcga tgtcaggatg cggcgcctga atcgaatctt tcgtcatcgc gacaagacga
     1261 cggatgtgct ggcgtttgcg accagggaag ggcccgggcc accctcgtct cttctcgggg
     1321 acgtcgttat ttccctgccg caggccattc gtcaggcgtg tcgtcatgag cagggcgttg
     1381 accacgagtt agcggtgctc ctgattcacg gcattctcca tttgtgcgga tatgaccacg
     1441 agcgcagtga ggcggaggcg caacgcatga gccggcgcga aaaggccgtg ctccgcgccg
     1501 ttgctccggt tccacggcta ttggtgtcac gcggatcgga tcaggtatga tccagaattt
     1561 gatcgccgtg cccgagaagt cctagtctgg atcgttcatc aatcattcga gtcggtcgaa
     1621 gagagggtga ggtacgcggt gggttggctt cagaaactaa gtgcggggct ctccaaaaca
     1681 cgtgatgcag tcacgggaca actggatcga ctcctggggc gggcggctga tccggcgctg
     1741 ctggatgagc tggaggtggc cttgatcagc gcagatctgg gcatgccggt cgtggaacgt
     1801 gtgatggggc agctgcgggc ccagatgcgg ggcggtaatt tctcgtcggc cgataaagta
     1861 cgagacctgc tgcggcaatc cgtgctggat attcttcttc cgacacaatc tgcctcgatg
     1921 gagcaattgg tggcacaggg gccgcgtccc tttgtgattc ttgccgtggg ggtcaacggt
     1981 gtcggcaaga ccacgacggt ggcgaagctg acgcaacggt ttcgacaaca ggggaaaaaa
     2041 ccattactcg tggccgggga taccttccgc gcggcggcga tcgatcaatt gcaggtctgg
     2101 gcggaccgca tcgaggtcga tgtgatccgc catcgaccgg gtgcggatcc ggctgcggtc
     2161 gcctacgacg ggatgaccgc tgcgaaagcg cgcgggtcgg acgtggtgtt gatcgatacc
     2221 gccgggcggc tgcacacgaa aacgaatctc atggacgaat tgcggaagat caagcgggtg
     2281 gtcgcgcaag agtgccaggg ggcgccacat gaggtgctgt tggtgttgga tgcgacggtc
     2341 ggccagaatg ccattgccca ggcgagacag ttccacgagg cggtcggggt gactggaatc
     2401 gccctgacca agctggatgg aacggcgcgt ggtggcatcg tggtggccat tgccgacacc
     2461 ttcaagatcc cggtccgctt aatcggcgtc ggggaagccg tcgatgacct ccaggacttt
     2521 gacgccaagg cgtttgtcga cgcgttgttc tagcccgcat gattcgtgac gggttcgtca
     2581 cgaactctct tgaagcatgc ggactggcct ttcctgacgg acggcggtct gtgatccact
     2641 ccagacgttt tcattcaatt tcttctcatc ttgtcgtgcc catgctaggc tcctgctaac
     2701 gggctgctac cgtctgcggc cctgcgcgac tggttgtgac gcacaaacga gataagatga
     2761 tgagcacgtt actggtgatc gacgatgatc ggttgcactg cgacttgttg cagatggcgc
     2821 tggcccgtca cgggtatcaa gtcagcatcg cgacgagcgg gcgtgagggt gtcgtcttat
     2881 ttcaacagct tcgcccgctg gtgaccttgc tcgatttgcg catgccggaa atggacggtc
     2941 tggcggtgct caaggagatc cgaacgcatg acccccgtgc cggggtgatt atgctgggtg
     3001 gcggcgcgac ggaggaactg gagaatcagg cgcgcgagtt gcgcgtcacg gatttcctgc
     3061 gaaaggggct gtcgctggat gtgctcatcg gggccgtcca tcgtgtggcg cagcaggcga
     3121 ggcgacaggc ttcatccgag cagtctcatg ccgggggaga gatagatcag cttcccgatg
     3181 agcagatcct tgtcgtcgat gacgacgtga tggcgcggga tcttctggtg agatttctta
     3241 gcctgcgcgg gtatcacgtt cgcgcggcgc gcgatggtcg tgaagcgttg cgactgatcg
     3301 gtgagtcggc gccggatctt ctgattttgg atctcgctat gccggagatg aacggcgtgg
     3361 aggtcttgcg gacgctcacg gcgcgagact atgccggcag gacgatcatt ttgggcggac
     3421 atcaaaacga tcccttgctg gccgaggcct gggcgttggg cccgcaagag gtcctggaca
     3481 agcccattga tttggagcgg gcgttgatgg ctatccagct tgtgatggtc tgtcgggagt
     3541 gttaatcttc accgtccacc tcagggtgcc ggtgaagggt gaatggtttg tatcgcctca
     3601 tccgtttcag tctcctcaca atttgtctcc ttcctgcgcc cgcagtcgct gattctgttg
     3661 ctccccctcc gacgattgaa caccatcagc tcgcgctcga gttgtctcct gattcccatc
     3721 gactgatcgc gacggatcga gtgaccgttg cgggaggatt tcccggacag gagctggcat
     3781 tttcccttgc accgacgctc ttgttggaac gggtcgagga tctcacgcat tgtcaggacc
     3841 cctgtgagtt cgccccctcc gttccatttg cacgggacag gtcgtcaagt caggcggaat
     3901 cgcagcgcat tctcctgaag cagccgcctg ccaaccaggc cggcggatcg atacggctga
     3961 agtttgtcta tcacggggtg attgatgatc ctccccggga tccacgccac ctgcggtttg
     4021 tcacgccgag cgaaacagcc ggtcacatcg gtcctgaggg ggtgtatctc agcagcgaga
     4081 gccagtggta cccggacctc gacgattcgc tggccaccta tgatgtacgc atcacgctgc
     4141 ctgaagggtg ggctgccgtc acgcagggca cggcccaggc tgacgcctcg cgatggattg
     4201 tgtctacgaa gagcgaagcg ctgacggtgg tggccaaccg ctttgtggtc aagacccgta
     4261 cctggaaggc gcaatccgga caggcgattc aattggctac gtatctgttc ccggacgatg
     4321 ccgcgctcgc cgacgagtac ctcgacgcat cagccagata tctcgacgcc tatatcccgt
     4381 tgctgggggc ctatcccttt tcacaatttg ccgtcgtcga aaactttttc tcgagcggat
     4441 tgggcatgcc gtcctttacg ctgctgggga gcggggtcat caagcgccac tatacgcaac
     4501 cgtacgccct ggggcatgag atcgtgcatt cctggatcgg caacggggtg ttcaaccggg
     4561 tgagtcgggg caactgggtg gaagggctga cgacctacct gtcgaattac tactatcacg
     4621 aactgaccgg cgatgaggct caggctcgtg aacaacggcg gctgatgctg ttgggctatt
     4681 ccgtctatgt gcgtcccggc gaagggtacc cgctcaggag gtttgcgcaa aagagcgatg
     4741 agaaagacaa tgccatcggc tatcagaaat ccgccatggt ctttcatgcg ctgagacagg
     4801 aagtgggcga tgcggcattt tggcgggcgc tgaagcgcat cccggaacag taccttggga
     4861 cggtagctga ttggagtgat ctggagcgaa cctttcagga agaggcgggt cgcgacctcc
     4921 gatggttttt tgcccagtgg gtcgagcgtg ccggtgtgcc ggaactcgcc ctgtccggcc
     4981 ttcgcctgca gactgtctcc ggcgctttcg gccacgcgaa tgcggtcgag gcggcgatcc
     5041 atatcgccca gagcgatgag ccctatcggg ccccggtcga gttggagttt gtgttgagcg
     5101 gggagcgcac ccatcgcgtc cgtgttcagt tgagcgccgc acaggaggat gtggttgtgc
     5161 cccttgcgga gccgccgtcg gcagtccggc tggatccgga ctatcatctg tttcgtcgtg
     5221 tggcccggag cgagatggct cccgtgctga atctgtatgt gacggatgga cagcgaagcg
     5281 tcgtccttgc ccaaggctcc cccgcgcagc ctggcccgtt cggcgatatc ctccagcgta
     5341 tcgtcgcaca ggaatcggcg aagcctgatg cagtgcgcac gatcgtgctt caaccaggtg
     5401 agggcaaccg atccgtaccg tcgggatcgt tgctggtgct gggagatccg cgcgagaatc
     5461 cgatagccgc agcggccgtg cggtcttgtg gtgaccatgt gcgtttccta gacggcggat
     5521 tttccgtggc gggtaagatg tatgaaggcg ccgccatggc gctgctggta tcctgtcggc
     5581 gtgaggagca tcccggcagc gtggtgacgc tgctgtacgg ggtgacgccc caggcgctgg
     5641 gacgtgtggc ccggctcttg tttttttacg ggtggcagag ttatgtggtc tttcaggaag
     5701 gcgccgtggt tgcacgggga gattgggagg ataggatgaa tacagaggtg cgaattgaaa
     5761 cacgttaaga cgatgatgat cggaatggtg ggcctcgcgc tggtcgccac ttcaacggtt
     5821 ttggcgggag cagccgagcc gaagtctgcg caggcaccgg cgaatgcgtc cgcctcggcc
     5881 gagcggcatc tgacgaatat ccggcagctg acattcggcc gacagaatgc cgaggcctat
     5941 ttttctttca gcgggaataa actcatcttc cagtcgacga acaattggat gaaagacacg
     6001 tttgccgcgg ctatgcatcc gtccgacatc ccgctcggct gttatcaaat gtacgtgatg
     6061 gatctcggga gcgaaaagat tcggatggtc agcaccggtg ccggcaccac gacctgcggg
     6121 tatttttttc ccggcgatcg ccgagtcttg tactcgtcca cccacctgcg agggcccaac
     6181 tgtcctccga agcccaagcg tgacggaggg gcctatcgct gggctttgga tgactacgat
     6241 ctcttctccg tccggatcga cgggctggac ccgcaacgtt tgacgaacac gccggggtac
     6301 gacgccgagg ccacggtctc tcccaacggc aagacgatcg tctggacctc tatgcgtgac
     6361 ggggatctgg atatctacgc gatggatttg gatgggaccc acccccgtcg gctgacgcag
     6421 gagattggat acgacggcgg ggcgttcttc tcgcccgaca gcaagcggat cgtgtatcgt
     6481 gcgcagcatc cgagcaatca ggaagagctg gatcagtaca aggcgctgct cgctcaaaat
     6541 ctcgtggagc ccgggcggct tgaaattttt atcatgaatg ccgacgggtc gaataagcag
     6601 caggtgacga ataacggcgc ttcaaatttt tcgccctttt tccatcccga cggccatcgg
     6661 gtgatttttg cgtcgaatgt ggacacgcgg aacgcagcga ggcgaccgga gtttcacctc
     6721 tatctcgtta acgaggacgg ttccgggcag gaacggttaa cgttcgaggg acagttcaac
     6781 agctttccca tgttctcgcc ggacgggaag accctcgtct gggtgtcgga ccggcatgcg
     6841 aaagagcccg gagagttcaa tgtgtttctc gccgattggg ttccatgatc gaaccaggtg
     6901 acgggcaggc ggcgtcgcaa ccggcgcagc ctgagccacc accggcctgc ccatcgatcc
     6961 cggcggtcct gctatcggtg attgtcttgt ttgggtcgtt tgcggcgctg ttttatatcg
     7021 atatccccat cctgtggttc ctccgctcgc acaatctatc ggcgctccaa tcgcttggtg
     7081 atctgggcga gaagctgggc aatggcggca ctctcgtgac catcagcctg gtgcttctgg
     7141 gggccgggta catgctgaag cgccaggcac tcacacgcgt ggcgctggat agcctcttgg
     7201 cgcacggtgc ggtggcgata ctggtgaacg ggctgaagca catcatcggt cgtcccaggc
     7261 caaggctcac acattcggga gggtggcaat ggtggccatc gctggattcc ggtctggatt
     7321 cgtttccgtc cggtcatacc tcagcgaccg tcgcggtggc gaccgtgctg gcgagggcgc
     7381 tgccccggta tcgatgggta ccgtttgcct tggctgcgtg ggttgcggcc agccgggtct
     7441 ggcggggctc gcatttcccg ggggacgtgg tggcggggat ggtgctgggg tttgtggtgg
     7501 ggtcgatctt taatggcccg ttgcgctggt ggggacggtc ctgcgggcag gcgcttatcc
     7561 gtatcactcc ggtagtccta tcgctgacag gtctcttctg ggtgctgacg catcgcatcg
     7621 ttgatccgat gacggatcac atcctgcttg catccggagc catgctggtg gccggaggta
     7681 tggcgttgcg aatggcagga cgtcggaatc cggcgggcga cggcagtgtg acgagagttg
     7741 cagcggcaga aagtctggtc ggattgggat taggtgtcgt caccggcgcc ccggtcgtga
     7801 tcgggttggc cggactattg tgcttcgcac gatggaacgg gggagtcgcc tgcgcggatc
     7861 cgtgctcggt tgcgccgcct tcctcctccc ggcacatcct ttatttggca ggagtcattg
     7921 ctgctgtggt cgtgatccag tcactcaaag ggctcgtacc gttgcagtga ggaatgggtc
     7981 agtgccctgg aatgcgaacc ggcggctgct ccgcctcttc cggcacatgg atcagcgatt
     8041 ggttggccag caggatgtag ccgtagcgct cgagtaatac cgggtagtcc atggtttcaa
     8101 acggcagcct gtttcgaagc gccgccggca ggagaatcat cgtgcgccct ggttgagtca
     8161 gatagggctt gatgttcgct tcttcattct tcggcgcgac gatggctttc cgctttgcat
     8221 agaacaccaa ggaggggcgt ggctgcccgt agaggatcaa ccgatcgttg gccccgagat
     8281 tgacgcctgc gacctcggcg agttgctggg gcggttcgat cacaaagtga tgcaccagcg
     8341 ggaagatcaa ctgtgtggtc gcgaggacga cgagagccag cgatccgccg gcgacccaga
     8401 acacggccgg tcgtctcgtt tcgctgaatc cgaagtaggc gatgaggccc atgcccacca
     8461 gaaagatgga ggcgacggta taggggcccg gtccgagcgt cacctgcccc gccaggggaa
     8521 attcatcgat cagctttcct gcgaacttgg catagagggg cggcaatgaa gcaaaggcca
     8581 gcgccaggat gcagcctacg gcggtgatgg tgtggatcgc agcgcgcagc cctggagtgg
     8641 cctggtcggt cacgcagcgg ttccagtagg acgccgtcaa gatcgccgcc gcaggaaaca
     8701 gaggaccgat gtaatgcggc aagcgggtcg aggacaggct gaaaaagacc aatccgccca
     8761 ggacccaggc ggcggtgaac cattcgagcg catgcggcga agggtggaca tccagcaccg
     8821 aggtctgcga cggaggcaag gcgccggatc gtttcgaatc ccgccagctg cgataggcct
     8881 ggtaccaggc gaaaggcagc aggccgctcc aggggaaaaa tccaagcagg aacacgggga
     8941 gataaaagac cagcgtgccg ccgtgccctt ccatggtgcc gaagaaccgg ccgatcgtgt
     9001 cgccctgtgc cgaggtcgta tatcgctgtc catggatatt cagcatgatg aggtaccagg
     9061 gcaacgccag gagcaggaac agcaggaggc cgggaatggg aaatccatga cgccaaaaga
     9121 gcccccacga gcgggtcagc cacaggtgca accctaccgc cagcatggga atcaggaagc
     9181 cgatcgggcc cttcgtcagg gtcgccagcg ccatgccgat gtagaagaac cacaggtagt
     9241 ggcgttcacg gccttctccg tacaaaccca gccagaatcc gtagagcgac aaggttgtga
     9301 agaagatcaa cacgctgtcg gtgagcgcca tccggctgag gccgatgatt tccaggttca
     9361 gtaacagcat ggccgcgccg aagaggccca ctactggccc ccggcagcgt gtcagaaaga
     9421 ggtattggag caggatcaat ccgacaccga agatggccga cgggaatcgt gcggcgaatt
     9481 cgctcacccc gaagacgtga taggagagac tcatcagcca atagacaaag accggtttgg
     9541 cgaagcgcgg ctcgtaattg aaggtggggc tgatgtaatt gcccgtttcg tacatttcgc
     9601 ggccggcttc ggcgttccgt ccttcatctc gatcggtcag tccgagggag ccgagtccaa
     9661 cgaagaagag cacggcggcc agtgccagca agaggatgag cgccagcggc tggatcgatc
     9721 ggtcggcctg cgccggcgtc tgcgaggacg ggtgctgtcc gttcatgtga agttccatta
     9781 ggatttggtc gccggggatg ccgtgccgga gtcggcttgg ttgggacgat gaatcagcat
     9841 gagattgcgg agatagacga tgctgccgat gctctgcccg gcgatgaaca ccggatcctg
     9901 ccgatagatc gcataggcca gcgtaatcag cccgccgatc atactcatgt accagaaggc
     9961 ggtcggcacg cgactttccg catggcgctc ggaggcgatc cattggacga cccagcgcat
    10021 aaagaagatc ccttgcccca ggaatccgat aatcagccag atggtgtcga gtgtcatagt
    10081 cgtgtggggg ccgaggccga aggcgatgat gtcgtcgcgg cggcacggta ttggtagcgc
    10141 aagcaacggt gctgcatcca tcgcacggcc accaagtcgt acaggccttt aaagaggcgg
    10201 ttgcccacgc cgtatttcga cgtgccccgg gtgcgtggat agtggcgcac aggcacctcg
    10261 gtgacggtga acccatgcat caaggcgagg gcgggaaaga atcggtgcat gccctcgaag
    10321 agctgcattt tttccaccac cgggcggcga aacagtttga gcgagcaacc ggtgtcgtgc
    10381 acccggtcgc cggtgaccgc gctgcgcacg ctgttcgcaa tgcgggagga aatcttgcgt
    10441 gtcaggttgt catggcggtc tttgcgccag ccgcagacaa ggtcgaaggt tttgatgtgc
    10501 ggaagcagcg tggcgatatc cgccggatcg ttttgcagat cgccgtcgat ggtcatgacc
    10561 agatcccctg tcgactgctt gaatccggca tcgaaggccg ccgattgccc gtagttccgg
    10621 tcgaagtgaa agaccttcac cgtggggtat cgagccgcca gtccatcgag cacgtctgaa
    10681 ctgccatccg aactgccatc gtcgatgaag atcaattcga acggggccga tcgcgactct
    10741 ccccggccgt ccaggaccgt cacaagttgc tcagtcagtg gcaccagatt ctcgcgctca
    10801 tccttgatag ggatgactac ggaggcccat gggcgggtca ctacagtcat ggagcgggaa
    10861 cagttccttc acgcagcggt ttatcggtgt gcacggggca cattctacaa aacgcatgga
    10921 ggggcggtca aacggttccg tccagggttc cgtctcagga tgtgtggccg cgctcacgaa
    10981 tcgagcgaaa tccgacgatc cacaaaggca gagaagttcg gatcgttgga gaggaagatg
    11041 accgaccagg gttcgtcttt tgcgcagaga cggcgaagca gcacctcgcg cgtggcgggc
    11101 agcatgttgt ggagcgtgcc gtcgaagatc agcagttgcg gccgtatcac aatcgcgcgc
    11161 gccacgagga gccgcaggat ctgactcatg gagaggttgg ttttcaggct ggacacgcgc
    11221 gtgttcaatc cctgcggcag cgcatcgatg tcgtggtcca gttccacgaa ccggagcgcc
    11281 cattggaggt cttgatagtc gatcgtgggc cgcccaagtg tgatgttgtc ttcgagcgtg
    11341 ccgtccagca gcgtgggatg ggagtcgagg acgagccctc gaacccggct gatcgaatcg
    11401 cgcttcacct ctatcagatt catatcgttg taccggacga acccggatgc cggcgggtgg
    11461 agtccggcca ggaccttggc gagagcggtc ttcgcgtggt tcgtgttgca gaggatcgcc
    11521 accttctcgc cgggggcgac atcgagggac acgttctcaa agatcggagt gccatccgga
    11581 ccggtatagg tcaggtttcg gcacgtcagg tggatgccgg ccgctccgaa ctgtgccgcc
    11641 ggcacgtctt ccttcgcgtc gtcctcttct gtgggcaggg agaacaccgc ggccatctcc
    11701 cggcaggaca cgaaggtgaa aaacatcgcg accatgcgtc gagccaaggt atccatgttg
    11761 atcagcaggt tgccgaccaa cacttcggcg gcggcaaatt gccccacggt aagttgtccg
    11821 tccgccacca ggagtccggc ggtgatgagc agcccgctgt ggccggcgac ttgccagagc
    11881 gctgcggctt tgtattgcct cccggtgagt gtgtcggacc ggcgttgtcg aatcctggca
    11941 tacaggcggg tcagtgtatc ggttcgttca aggagatagg ggctgtcgcc ggcggctcgc
    12001 agatggggga gattgtgcgc gatgttttga atccacccga agatatcgta gtggagccgc
    12061 gacatctcca gagtgatgaa gaagccgccg cgcccgaaga gggtcagtaa tcccacaaag
    12121 cctagaatca gtacgagaat gtagagaacg aagaaggggt gaaacaggat cagcatggtc
    12181 acgccgatgg taccgaccac ggcgacgtta aacagatccg ccaccatggc taccaatgcg
    12241 cgtgtcagca ggtcggcctc catgaaccga taggcctgcg gcgtggcgaa cgtatcgtcg
    12301 cggaggcggg gcaggagacg tgtgaacccg atcgcgatgc gcgtgtagat ccgctgttgg
    12361 aatgtctcca ctgcgcgcgc ctgcagcaca cggaatgccg cgacgccggt cagcgaactg
    12421 gcgacgaaca gggccagtgt aaagatcatg cggggttcca tcgcaaagga gaacgtgctg
    12481 acgagctcct ggacggcaat ggggacacag aggaggaaga agccgatggc aactgcatac
    12541 gaggcgatga tgcccaggat cgatcgctcc agcttcagca gcatgcccag gtgggcgaaa
    12601 atatcgcgaa ggatctcggt cagctgatac tggggatagg tcggggttgt tgtggggcgc
    12661 tggttcacag aaactcctac cgcgttgggc gctggtagga gttatcagcc gaatgcgagg
    12721 atcgcgaccg gcggttctcg gtgaactcca tcaccgggtg gactggctgt attagtgcga
    12781 agtctagacc gagactctct agttcgtcaa cacatagccc gcttcatggc ctcatattcg
    12841 ttggtttggc gtgagctaag ccgagagctg atcggtccat tgtcggtgga gtacgacgcc
    12901 ggcggagttt ttccgcatcc ggttagcgga ggaagaggga cttgaagatc agggccgtgc
    12961 cggacaaaaa gagaaatgcg gcgaaggtgg tgcggtatcg ttcggtgctc atcttgctat
    13021 agagcagcag ttcggtagcc agaaacatgc tcacgcccac tgccatgtaa agtgcggtgt
    13081 cggaggtggc aattgcagaa ggaccgaggg agatgcccaa cgtgacgagt tgaaacagcg
    13141 cgaacgtgcc gtaggacagg gcggtcgtgg cgcgggtctg atctttgtcg tactgtttgt
    13201 tgtggatgat ggccgtcaac agcgagccac ccagattggt caggccatga acaacgccca
    13261 tcacgatgaa ataggtgcgc tcatatcgca ccagcgactc gatcaaccga ttgaacggca
    13321 catagacgct cttgagcgcc gcgacgatca agaagagccc gacaatgctg ccgatgttca
    13381 gcttgagtgt tgtgaccagc aggagacaca gcaccacgca gggaatcgac agggtgagga
    13441 tctgccggta gaagggcacc tggacgtgtc ggtagtcctt gctgacttgt agcaggttga
    13501 tggtcagcga gatcgggagg aggatagtca gggcggtgat gaagtcatag ccgagcacga
    13561 ggaggatcgg agtcccgaat agtagtactc ccacgccgaa gagcgactgg atggtggccg
    13621 tcagaaggat ggtcgcaaga atgtcccact gcatggcgat gatgctcctc tcggaccgaa
    13681 cgtgtccaag atacaggctc tcaactcccg gtccggtagc ccggtatatc ccagctttgg
    13741 agcgggcgtc aatcagtcca caggcggggg cgtggtttac cggctcctgg caggggctcc
    13801 caaatccgac gaggcggtga tggtgaagcg catggtgccc atcaggctca tgtcgttgat
    13861 ctgttcgccg gcatggattt ccaccttata ccgtcctggt ttccagccgc tttccgtcgg
    13921 gaacagcttc aggtagccgg tttggtcctc taaggcgata tacatcgcat cttcagcgac
    13981 cacggttctt ccgtccagcc ctgtcacttc ctccgggtag cagcgcccga atacttggaa
    14041 cgattggtaa tgctgatgga gttcgaagac gatgaagacc ggtctggagc cggcggcgaa
    14101 ctgttcggtc ggtctgacgg gaacaatttc atgggttcgt tgaaatccga gctcctcatc
    14161 aaacccttcg gcgagcacga tgtggcggaa catcccttcg ggtggggcgt cgaagttata
    14221 gggtttcgcg ctctcggtgc ggttctggaa atcgggaatc ggcacatttc gggtgagcgg
    14281 gagatcttct gcccagagaa gggagccgga gagcaggaag atgggacagc agaaccagag
    14341 caactgcctc atcccgtctt ggtacactcc gcccagccgg cctgtcaagg agaggctctg
    14401 cgccgggcct ctggccggac ggtccgcttg gtaagaggga ggtctaagcg gcgaggcggg
    14461 agcgatgaat cgtgcggggc aggttgcggg tgaaaattgc ttcctgttag actttcctcc
    14521 tttctacggg gggacagaga tggaacagca cctcatgacg cagttagagg atcccgacgc
    14581 gctgccgcag ccgctcgggg agtataagcc ggtcgattat tggcaggccc atatcaacac
    14641 cctgttttat caactgcggg gcgatcagca acgcagcttc tatcagacat tcacctcggc
    14701 cgattatcga ttggcacatg cgctggccgc cgactacttc gagcaggtca ccaaacgcga
    14761 caagaaagtc gccgccaatc gtatgacgtc aaacggcccg actgcgacgc cgtccaccga
    14821 cgccactcct caagcacagt tgacggtgat ggagtggggg ccgggtaacg ggaatctggc
    14881 ggcatgtttt ctcagccagc tgcagcggct cgacaagaat gggcaggtgt atccacgagt
    14941 ccggtatctg ttggtcgatt cccaggccca tgcgttggag cgggcgcgtg ctcacccgga
    15001 tctggctccg catttggcga aggtggagtc gctgtgcgcc gaggtcgaga acttggcgac
    15061 tatcgccgac ggcaccgtcg atcgcattct gtgcaacgaa ctctggaacg agttggcgac
    15121 caagctcatg gtgaagaagg ggggcgagtt cgaagaggag cacctccggc cgaacctcaa
    15181 cgagcgaaag gccgccgcca ttgccgattg gtcgggattt gtccgtgcct ttgaggcgaa
    15241 ggacattgag cgcttgaagc agtttccgcc ctttctggac gatctgatct gggagcgcga
    15301 gtaccacaag gtggactgga aagacgtccc ctatcgcaag acgatcaccg agttcatgaa
    15361 agcgatcgac gatgaggtgt tggtgccggt caatctcggc gcgtttgcat ccctgaagga
    15421 agcgaagcgg gtgttggccc aagatgccgt gggattcagc agcttcgatg ccgggacggc
    15481 tgacatggaa gtcctcaatg atccggacaa gccctgctat ggccagttcg gtggccagta
    15541 cagcttcatg gtgaatctgg ccctgattca ggctgtggcc aagcatctag ggctgaacgc
    15601 ggtgacgatc gaaacgcagc gggagttcgt cgggagccgg ctggggacga atgtgatgac
    15661 gctgatggac ttgctggcct gccacccgat ggtcggctca aaggttcagc cctgggagct
    15721 ggatcgcctc acagtcaaaa ccatccgcac gctgaacgag acttatgaga gtccctatca
    15781 acgcaagatc gagttcccgt tgcgcagtga gatgccggcc gaggagcgcg acgcggcgca
    15841 gggtattctg ctctcgctca aaccgaacgg gattcccgat accatcgcgt atgtcaccga
    15901 agaagaattg agccaggcac aaccggagtt ggagaatctg ggctatgaac gcgaggcggt
    15961 gttgatggcg ctcggggcgc ctccgagtcc tgtcgagtac tatcactttg catgtcggcc
    16021 gtaacccgcg ctggatcgtg cggcaggacg ggcccaccag gtgatgtctg taggagaaag
    16081 agcttgacta attaattcca tttctatagc ctttcccgcg atttcgaaga gaagacccgt
    16141 ctcccctccg tgcatcgcga gcattcacgc gggatggggt ctcgcaggca agatcatctc
    16201 agacgacgtt cttcacgatc cggcctttcg gtcgcgagtg cgtgatatag acgtcgtgcg
    16261 aataatcgca cagctgtaac aaggagcata cagtaccatg tttggttcgt ttggctggat
    16321 ggagctgttg ctgattctca tcatcgtcct gatcatcttc ggtgcgggga aaattcccca
    16381 actcggtgag ggattaggta aagcaatcaa gggattcaag aagtcggttc acgaagcgga
    16441 tgccattgac gtgacggcta ccgaggcgga acccgccccg actcagccgt cggcacagat
    16501 ccagcaaccc ggacagccgg ccacaccgcc gcctgcgcag caggccggtg cggctccacc
    16561 gccacggacg acgcaggggt aacttgcgaa actgaggatg cagaccgaat gacggcgatg
    16621 tgacctcgga gtcccgccgc tcggtcttca gtacaccccc cctcagtatg gaacaccatg
    16681 ttcggtcttg gcgctggtga aattctcata atcctggtca ttgcgttcct gctcttcggg
    16741 cccaagcagt tgcctgagat cgggcgtcag gtgggcaagg ccgtgaaagg gtttaaagaa
    16801 acggcggacg atctgaagaa gaccgttgag ccggagctca acatgattca gcaggaaatg
    16861 aaaatggtgg agcaggattt cgagtcgtcg atgaaggaag cagaagaaca gattaaccac
    16921 gcaacatcgg gcgtagagca cggggcagag gaatcgggtt tgcccaagca ggcctaatac
    16981 catttcatat ccgctctacg gctgtgaagg gaggtgacca cagcaacaat acaagacctg
    17041 acggtgtgag gagctcgttt cgggatgaag cgaggatcgg ggccgaatgg tgggcaccat
    17101 tcaggttcga atgtcccgac atcacacaca aagtgggctt tgtagtgcaa tagcggttgg
    17161 atgtataggc ggcacccggc gtgagtgggt acacaacggg ttgccatcca attcacagga
    17221 ggaacgctat gaaacgcatc catggacgga ccagatgggg ccggttggcc gccatcttcg
    17281 gcgctgcggc agtgacggct ctgcagtgtc tgacagtccc ggcttttgcc ggatttgaac
    17341 tccccccggg cgaacgtatc accaaccttc ccgccattcc tcgtaacatg ccgcagaagg
    17401 aagcgtacga aatttacgat ccggtcatcg gccggaactt cgacatcaag aatctctgga
    17461 tgcgcgccga tctgcgcgtg cggccggaaa tgcggaacaa cacctgcttc ggcggtggga
    17521 tgggcaccgg tggtgcgtgt aacacgttcg gtggtgcgaa tgcgcgaggg gtggccccga
    17581 atgtgaataa taacggcaat aaagggaacg atttcttcgt ccagcaatgg atgcgtctgg
    17641 gcatcgggta tgacctctcc ccggacgtga acttctacgt ggaaatcatc gacagcgcca
    17701 cctggggcgg caatggtagc gcggtcaatg caggaaacgg cggtgatccc ctcacccata
    17761 acggtgccag cgctggcggg gccggcaatg gcggtcggtt aggcgtccgt gccgcctaca
    17821 tgttgatcag aaatctggcc gatgtgcagg gcctgagcgt gaagatcggt cggcagtacg
    17881 tcgtgttcgg caaccattca ttgttcggcc acttcgattg ggccaacacc ggctattcac
    17941 atgatggtgt gatgttcgcg tatcagacca aaaactggga cagctacttc ggctggttcc
    18001 gtaattcaga aagcgacctt ggccaggcgg ctccggtcgg aagcggcgca gccaatatcg
    18061 ccggtaccgg cggtcaggat gcgcagcgcg atgccgacat gttcattttc tataaccaga
    18121 tcaagatggt ccccggaatg gtcatcgagc cgttctatgt gttgtatcag aatcgctatg
    18181 ggtcagcgga taacgcgaca cagggacttg gtaccgccaa gcactccaac cagaccagac
    18241 atatgatcgg aaaccggatt gaaatgcgca agggcgggtt cgatttcagc aacgaaatcg
    18301 cctatcagtt cggccaaatg ggccaggctg gagcttgcca aggcgaacag aaatgtttgc
    18361 acatcaatgc ctgggcgacc agaaactgga tcggctacac gttctacgac acggcgtgga
    18421 agacgcgcat cgccttcaac ctcgattatg cctccggcga cagccggaac aacacctgcg
    18481 gcgcaacggc tgggtcttgt aagacagcga atacctttga aaacttcttc ccgacgaacc
    18541 acatccacat gggctacatg gacgtgcaag cgtggaagaa catgctgtct ccgtccgtca
    18601 acttgcaggc tcgtccttcc gcgcgtgacc atattgaatt gtggtacacg aacctgaacc
    18661 tcgccaattc caaggattgt tggtatcgag gtgctcaggg ttgctacgtc ttctccaacg
    18721 ccaacaatac caagacgcac attggtgatg aaatcgacgt cagctacacc agaatgtttg
    18781 ccgatggcaa agtggcgctt caaactacct atggcacgat tttcagcggt ggctatctga
    18841 ccagcaccct gaaccagacg caaaatcaac actgggctta tgtgtcattg tggatgaact
    18901 tctaggaaga agcgatcagc tcattgctgt cagccatcaa ctgctgagtg ccgcaggctg
    18961 atagcgaaag caagacaaag gagatcatga tgaaaaaact catgttcgta acgctgggca
    19021 tcgcagcgat ggcgatcggc agccagccga tcaccgcgca ggctcaggtc gcggatgcga
    19081 ttcaggcagt gaacgatgcc atcgtcgagt tgaccgatgc gccgggcctc ggcaagcgca
    19141 cgaccgtcga cctggcaaag cgatggggag ccgaccggtc cgttgtcgac agtgccacgg
    19201 caaaactgca ggacgggttc agtaaggccc agtcaggcgg agccggggct gatgccatgc
    19261 gtcagttgaa actggcggtg gattacggca aggctcgctt gcacaaagag gcccgcttgt
    19321 ccgcacaggg tgctttgcgg catctgtgtg tggcgaacca ggatcaaggt cccggatgcg
    19381 acacggtgcc gaagttcggc agctacaccg cgccgtagag tgagtcgacc gacataaggc
    19441 gaggcgtgag ggagatcccc tgcctgtctc gaagagtgaa gccccgccgg gtgatccccg
    19501 gcggggcttc ttttttcctg catgggtggg gcaacgggtt atggtgagag cgctggcttg
    19561 cccgcagtca gtcgatcgcc ttatactcgg cgcactcgta atcttcatca ccgttcgtgc
    19621 ttttctttcc tcatcgtgga cgatctgagc acacagctga gccggacatt cgggttttcg
    19681 gcctttcgcg cggggcagcg cgaagtcatg gaagccgtgc tggcccgtcg cgatgccatg
    19741 gcggtcatgc cgaccggcca gggcaagtcg ctctgttacc agcttcccgc caccctgttg
    19801 tccggggtga ccctggtgat ttctccgctc attgcgttga tgcaggatca agtcactagt
    19861 ttgacggcgc gggggattgc cgcggcggcg ttccactccg ggctttccga gcaggagcgg
    19921 gatcgagtgg tattggatct caagctgcgc cggttgcagt tgctctatct cgctccggag
    19981 cggatgcagc atgagcggtt tctccgtctg ctccgttcgc tgtgggtgtc gttactcgtc
    20041 gtcgacgaag cgcattgtat ttcccagtgg gggcacgatt tccgccctga ctatctgaac
    20101 atcggccggt tgcgccggga actcgagaat cccccttgcc tggccttgac ggccacggcg
    20161 accgcgcgcg tgcaggcaga tttgtgcgac cgattgtcac tccaggaccc gcttcgcttg
    20221 gtgactggct ttcgacgctc taacctcgcg ctgtctgtcc gcctctgccg gtcccgccag
    20281 gaaaagctcg cgacattgga tcgtgtggtg cgtgaatacg aaacgggcac cattctgatc
    20341 tattgcgcca cgcgtcgagc ggtggaggag gtggcggcct gcctggggca gtcgcagtcg
    20401 tccgtcgggt actatcacgc cggcttgtcg gatgaagagc ggcggaaggt gcatgacgag
    20461 tttcgtgtgg gaacggtgag gattttggcg gcgaccaatg cgtttggcat ggggatcgat
    20521 aaatcggatg tccggttggt ggtgcatttc gatattccgg gaagcctgga ggcttactat
    20581 caggaagtgg gccgggcggg gcgtgatggc cgacctgccg cctgcctact gctgtttcat
    20641 gaacgggatg tggccactca ggagtacttc attcagcaag cgtcgaaaga atccggtagt
    20701 tcagcacgcg ccgatcgcat gaagacgttg ctacaggatt tactggagta tgtgtcggtg
    20761 ccgacctgtc ggcaactcgc cattctcgac tacttcagcg atgaggccga acgggccctt
    20821 gggccctgcg ggctttgtga tcgttgcgtg gtgacgccac agcccagtgc gcacgttgtc
    20881 ggtgatgagg ccacctgcgc gaaggctgtc ctggcggcgg tctcctggtg tatgggccga
    20941 ttcggtgtga gccgaattgt cgacatgctc cgcgggagtc gatcgaaggc gctgctcact
    21001 tatggggccg aagagtgtcc ggggtatggg gggtatcacg catggtcgaa gacggcgttg
    21061 actcatctgg tgaaagccct catcgatgcg ggatatctgc aggtcgaagg actggagtat
    21121 cccacgctcg acctgacgcg gaagggacag gaggtgttga aaggaatcag tcctctggta
    21181 ttgagcgagc aggctgagga tccggcagcg tcgacgctga aaccacaagg gagcgggcga
    21241 ggcagctcga tggcgcctct gcataccgcc tcagtcgacc cgcagctctt tgaacggctt
    21301 cgtcaactcc ggcgtgagct ggccgaggaa gaaggggtcg cgccgtttgt tatttttcat
    21361 gacaagaccc ttcggaatat cgccggttac aaacccgtga cgcttgccgc cctgctggag
    21421 atccccggga tcggcgaggt caaggtggag cggtatggcc gccgagtgct gggcgtggtg
    21481 aacgaggcct gcgactaagc cgtcttctcg ttattgtcta acgacccctt caatgccatt
    21541 ttgccgggga agaatgattt cgtagccatt tggctattat tctctcagat tcccagcctt
    21601 tcgaaattgc tcctgcagtt cggcatatgt tttcgtcacc ggaaactgag ggaactcttt
    21661 cgggagatgg tcaggggggc ggaaaaagaa tccggcgtga gcctctccca gcatggcggt
    21721 gtcgttgtag gcgtctcctg ccgccatgac acgaaaattg aggttcttca acgcggccac
    21781 cgcatgtttt ttctggttcg gctggcggag tttgtagtgg acgatcctgc cggtcggccc
    21841 aatctccagt tgattgcaaa aaatcgttgg atatccgagc tgccgcatca agggaagcgc
    21901 gaattcataa aacgtatccg acagaataat gacctgtgtc cgctcccgca gccaggcaat
    21961 gaattcggct gcgccttcca tcggccccat ggcgtcgatc acggattgaa tgtccgccag
    22021 ggtcaggcca tgctgatcca ggatgctcag gcggcgtgtc atcagggcat cgtagtccgg
    22081 catctcgcgg gtggtgatct tgagctcttc gatgccggtc ttgatggcaa agttcaccca
    22141 aatttcaggc accagcacgc cctcaaggtc cagacacaca atcacggggt tctgcattca
    22201 tcctcctgcg ggaattgatg ggtggtgaaa agtgggcttc tcggtaactg gtagtgatag
    22261 ggacaaacca gcggtctgcc ctatcccgcg ttactacgac gggccttttc ttgactgaga
    22321 taccgccaga tcttgtccat ggccttctcg aatagttctg tgccggccca ttggttggtc
    22381 tgctcctcct tatgccgtct ggcccaatcg acggccagcg ggagcgggat cagcctgggt
    22441 gctgttccgg tcaggtctgc ccagagcagg ctcagagccg tactgaggcg aacggcgacg
    22501 ggaaccggct ggacggtcgg cgtggtcata tcctgacatt ctgtggccgg cgacgtgaca
    22561 gcaagctctc ggcaggctgc aggccgatcg gcgtaaatgc tgcaggtttc gttgtcgaga
    22621 aaggggcagg gcatgcgaag cgcgtagtag gcccggttca acggctcgat cgcctcgtca
    22681 ctcaacggct cagaagaatc ggccagcgaa gagagttgtt tcaggatacc ggcgcgagcc
    22741 agttgttgct gggccaggtc gagtttggac agcaggcgat tccgctcgtt ctggtccagc
    22801 cggtcgatgg tgttggtgag agcaaaggct tccggcaccg aaatcggtac gagcatccgg
    22861 cagcaggcgg cgcagccctt ttcgcacgag accctctcgc cggcttggag taatcggcgt
    22921 tgttccagga cctgggcttc ttctccgaga ctgcgcatga gcgggagaat cgcagtgatc
    22981 ggtacgaagc tcgttggcac gcccacggca gtgctgattt ctccgcttgg agtctgcaga
    23041 gagatttcaa agtgttcaac ggcagtgtgg ttactcacgg gtacccatct ttaagtccga
    23101 atgggagtca cttgcgcgat ggcattcgtg ccatcctgcg caggcgctat catagagaac
    23161 ctcagaatca gtggtcaacc aaagcctcat agccctcagt cttgcccc
//
LOCUS       DAORRQ010000061        23178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_133_length_23178_cov_16.650910, whole genome shotgun sequence.
ACCESSION   DAORRQ010000061 DAORRQ010000000
VERSION     DAORRQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133_length_23178_cov_16.650910"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PK224_16120"
     CDS             complement(<1..708)
                     /locus_tag="PK224_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB17321.1"
                     /translation="MKMMIAVDGSEFAEWSVQMLEAVASRPPDSVTLVHVVDSASLKS
                     AARKHAAVSKQAIAAMTKAGDQILRRFESLAKIALKQATTKPRTAIDTILAHGRVADT
                     ITKLAKQKKADLLVLGSRGLSDAEHYLLGSVSRKVSALAACPVLVVKRPLTALSHVLF
                     AADASKHSQGACRFLCQRFLPESAHVTVLSVVEPAMTELAAKYLSKDQVEQISVPKRQ
                     AAEQVVENLRDRFLAEGC"
     gene            1015..1548
                     /locus_tag="PK224_16125"
     CDS             1015..1548
                     /locus_tag="PK224_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17322.1"
                     /translation="MFESRVIAVMSGMVGLSLWLAGVEPVAGGVTTRVAPGGDAIELE
                     IPTEVGTQKETLPLYHSGKIHYFSAGIGQVEREAAYPPFPLKLVFTAGGKPFVTGVEV
                     VLRQSKGATVLTVPREQVTGPWLFIDVPDGAYEVAATLGGQIQQVKGIHVRRGHVTTQ
                     HVRWAEDRSPVLPAQAE"
     gene            1592..2065
                     /locus_tag="PK224_16130"
     CDS             1592..2065
                     /locus_tag="PK224_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB17323.1"
                     /translation="MVKKRKAPARRSAGAGRRRVAFEDLTVGDIVNKRVQAARLDMKG
                     DRVAKLLLKEGSNVPVVDQAKQLTGVVSEHDLLSALDEGQAWSDQTAKDLMTENPYSV
                     PPETTLSTLIHVLTESDLMSVPVVNAGNRLVGVVTRRDVVRAALRPGVKRRAKGR"
     gene            2103..2537
                     /locus_tag="PK224_16135"
     CDS             2103..2537
                     /locus_tag="PK224_16135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB17324.1"
                     /translation="MRRIDLITQACDPKTLTVGQLMQDALFTCTAQTDALTIGRLMTK
                     QNFGGLPVVAEDGSLVGLVTEYDLLQAMIEGRDLRKVFASEIMTTQPLAARENMTLEE
                     VANLFQDRYVTRLPVVRGKQLVGIVARRDLLHGYMKASAYWS"
     gene            2724..3584
                     /locus_tag="PK224_16140"
     CDS             2724..3584
                     /locus_tag="PK224_16140"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRB17325.1"
                     /translation="MMPEQAVTATARVVQRVVLMVVLFAPGTLPGEAAMPRRAAETRS
                     PCAVHHPSDERVAWHCRRLQRGETLETLFGRRWVDVARFNRIDRRHAQPGREIKIPIR
                     LDDVAEFTPLPGRYPAGEQDQQLIVVDLTEQFIGAYEYGLLRLSFPIASGEPENETPV
                     GDYRITAAHRTHRSCLYTIESTPVPYPMRYALRFYVNAQGVSYWIHGRDLPGYPASHG
                     CIGLADEQMQRQYYGVPPTPVLEDAKRLYEWVIGDRADSGTLLPLSNGPRVVILGSLA
                     KRGDAGASMP"
     gene            complement(3581..4786)
                     /locus_tag="PK224_16145"
     CDS             complement(3581..4786)
                     /locus_tag="PK224_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB17326.1"
                     /translation="MMDTSPPHSLRRMPTGIWVLGVVSLLMDISSEMIHSVLPLFMVT
                     TLGAGTIAVGIVEGLAESLALVVKVFSGTLSDYLGRRKGLALFGYALGALTKPLFAIA
                     PDIGTLLTARLLDRVGKGVRGAPRDALVADIAPAHLRGAAFGLRQSLDTVGAFLGPLF
                     AVGLMLLWANDFRAVFWVAVIPGMMAVALLLFGIQEPESRQTTTRANPITRDNFARLG
                     SAYWWVVGIGSIFTLARFSEAFLVLRAQQGGIPIAFIPLVMVAMNLVYASSAYPFGKL
                     SDRMNHQTLLMLGLLVLVAADLVLAMNDHWSVIPAGVALWGIHMGMTQGLLATMVADV
                     APTDLRGTAYGCFNLACGLAMLIASLLAGFLWDRLGAAFTFYAGACFSVLAIIGTLSS
                     PMTQHRRTG"
     gene            complement(4876..5286)
                     /locus_tag="PK224_16150"
     CDS             complement(4876..5286)
                     /locus_tag="PK224_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17327.1"
                     /translation="MNTTILTICLSAVLTLCPAWPDTSHADSSLPNEPGEELLVAQSS
                     DTIIGLLFRDYSLRGNGQVDYRTARHILGISYDDPASEEPDVALFPLFYWYDANQDGQ
                     WEMWVDRDETGRLTDAVRYDWRQGQELITSSKTW"
     gene            complement(5780..7210)
                     /locus_tag="PK224_16155"
     CDS             complement(5780..7210)
                     /locus_tag="PK224_16155"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRB17328.1"
                     /translation="MNQHSVSSTIVAGWVVAALLSLPTGADAAPKPAPKPSSGNTCES
                     AFKQTRPASKILDKVLQSHARWLEDRDSPDGRRANLCRTDLRQLRLVGANLERINLEG
                     AILKGSNLRTASLVQAHLKGADLSQAVLDDANLEGADLRKALLVKAHLNRIAADEAAF
                     YGANLQGALFREALLERAHFEDADLQGADLSNATLLDGYFYGANLSKANLTDADLAGT
                     DLRRTNLRQANLRRANLQGALMDSANLDGASLVEADLESAYLDDASLAHADLHEASLR
                     GAGFRFTHLGGANLQRANLENANMEGANLVKARLDSATLTMTVLYKANLSAANLHGAS
                     LHHAVLIGTQLARADLRKADLTEIYGPNAHLQQAWLSEANLELANLVAADLSQADISH
                     AVVVQTNLQETNLRGANLSASDLTGALLNNADLGQADLRGANLSGALGLVQAQLDAAC
                     LDEATRIPSELQRPKPCAQPRPRLRK"
     gene            complement(7278..8612)
                     /locus_tag="PK224_16160"
     CDS             complement(7278..8612)
                     /locus_tag="PK224_16160"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRB17329.1"
                     /translation="MNITSDALLTDLYELTMAQAYLAQGMTDIAVFEFFVRKLPPQRN
                     FFMAAGLEQVLSYLEAFQFSDADIAWLDQTGGFRPASLNALRAMRFTGEVHAMPEGSL
                     FFPHEPILRITAPLPQAQLVETRVMNLLNFQTMVASKACRSVLVAGDKPLIDFGLRRA
                     HGAEAGLLAARAAYLAGLAGTSNVSAGARFGIPIYGTMAHSFVQAHLDEAKAFIHIAE
                     TQPRNVVFLIDTYDTEAAAEKVVSLAGRLNARGISIHGVRLDSGDLADHARKVRHILD
                     RGGLTATQILASGNLDEERIQTLVTGQAPIDSFAIGTAMTTSADAPYLDCAYKLQEYA
                     GRPCRKRSEGKATWPGRKQVYRRIGTDGRLNGDIITVEGDCREGQPLLGHVMQGGQRV
                     ASPPSLEDVRRYARLSLTQLPAPLRRIDRADPYDVRIAPALTELAEQVDLHT"
     gene            complement(8609..9190)
                     /locus_tag="PK224_16165"
     CDS             complement(8609..9190)
                     /locus_tag="PK224_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRB17330.1"
                     /translation="MTYLTPHDALVVVDVQRDFLPGGALGIQDGDRIVPILTRYVARF
                     HARGLPIFITRDWHPPDHCSFREQGGPWPVHCIAGSPGSLPPPNFQPPVSAVTIYKAI
                     DQDKEAYSTLQDTPLDRHLRALKVRRLFIGGLATDYCVLNTVKDARALGYDVCVLMDG
                     IKAVNLHPDDGRRAEEAMVLAGAIPLCLEQLAA"
     gene            complement(9242..9622)
                     /locus_tag="PK224_16170"
     CDS             complement(9242..9622)
                     /locus_tag="PK224_16170"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB17331.1"
                     /translation="MQIVMTTGTRYSFVVSLVVTLAAACGPATAQDFPPDVTRGKAVY
                     ERHCLACHGRGGWGDGPDAAALTVPPANFHRFKSFLKSDEELLRTIEHGIVFSAMHSW
                     QGQLTETERLDVLAYIRLLVQQGK"
     gene            complement(9640..9804)
                     /locus_tag="PK224_16175"
     CDS             complement(9640..9804)
                     /locus_tag="PK224_16175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRB17332.1"
                     /translation="MPMYDYTCLDCGKESLIIVTLKQHEKGEVQCPACGSTKLQQHFS
                     SFIAHTTKKS"
     gene            complement(9821..12235)
                     /locus_tag="PK224_16180"
     CDS             complement(9821..12235)
                     /locus_tag="PK224_16180"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRB17333.1"
                     /translation="MSDKQKLPASLLAPQVDPGRLGFDDTSEVEPLDDTIGQERAVEA
                     LDFGLQMKSPGFNMFVSGPVGTGKGTLVRQMVKRLAQTAPAPFDWCYVHNFQDASRPT
                     CLSFPAGQGASFKREMSTFIEGLRRDIPAAFEGKKYLDAKAKIIEETEGKKKALFHDL
                     TALCHQRGFGFEETPVGFGLVPLKDDRPMTEKEMEALSEQVQQELTEKRQSLESDLRE
                     FHVRMHTLEREAEQALHHLDHQIVANVMKGAYETLQRAYQDLRPVTTYLQRVHHDIIH
                     QYKDFLPHSGPVLPIPGLEQTRRPDMTRFAVNLIVAREATAGAPVVDEPHPTYSNLIG
                     KIERRAHLGVMYTDFTEIRAGAMLQANGGYLILQALDLLRQPFSWDALKRVIKTGAVT
                     IEDPAEFYGFATAGLRPEPIPVSVKVILVGTAGIYHLLQAYEEDFSKLFKVKADFDVE
                     VPHDERQERQYARFVAKLCKEEGLPHFGADAVAEVIRQGFRYADRHDRLSLRFSLVSD
                     LIREAGYWARKDGHSFVTRADVESALAHQRHRADLPEQWIQDEIREGTLMVDLQGEVV
                     GQVNGLSVYELGDYAFGRPTRITARTYVGTKGVIDIQREVELAGNIHSKGVMTLAGFL
                     AGKFAGMQPFALSATLTFEQTYSEIEGDSAAVAELTAVLSSLADLPVRQSLAVTGSVN
                     QLGEIQPIGGVNEKIEGFFESCKRRGLTGTQGVIIPARNTKHLALNREVVAAVEEGKF
                     AVYGVDHVEQALELLIGVPAGERGPEGTFPPESIYGRTAARLEEMAQIVANWGEGEEE
                     PEKITG"
     gene            complement(12247..12792)
                     /locus_tag="PK224_16185"
     CDS             complement(12247..12792)
                     /locus_tag="PK224_16185"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRB17334.1"
                     /translation="MNLEVESRNIAMTPRWKTEIEERMATLQRGHDDIIHGRVTLTKN
                     RHHKKLDNVAEALVLVTVPTRHTITSRKEDKTFEEAIRAAFDAVAIELRKYREKRADK
                     VVRIEPLPQLRGVVSKVFPHLGYGFILKDGGGEVYFHKNAVKGIPFNDMEDGQEVLFE
                     SEPGEKGLHATIVQPPHALEV"
     gene            13075..14160
                     /locus_tag="PK224_16190"
     CDS             13075..14160
                     /locus_tag="PK224_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRB17335.1"
                     /translation="MPVLKKSALETYLKARFGPSAELLAYGPIGKETNGARYKQYGYG
                     APVRLTFRQGGKTRRVVLGTMSPGPFGHEHPADRAQAMLWDYDCYGRLPGHIDALDVG
                     AFTARGELMSVSAAKEFFLLTEWSEGATYHKDLERMAGATKPTALDRKRTGVLAGYLA
                     DIHRVKQTDPQLYRRRLRELLGHGECIMGLTDSYPDRFAFITEDLLSRIEVACNVWRW
                     RLRSRHARLSQVHGDFHPWNVLFRHGTDFSVLDRSRGEWGEPADDVTSMSINYLFFSL
                     CRHGTLKGALEVAFHSFWETYLARSRDHEVLEATAPFFAFRGLVLASPLWYPKLTVTV
                     RRKIFRFIENVLATARFDPADVNRYCE"
     gene            14169..14696
                     /locus_tag="PK224_16195"
     CDS             14169..14696
                     /locus_tag="PK224_16195"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF019544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRB17336.1"
                     /translation="MTPLPFALWLTGLPASGKSSIVARLLPRLESWGVPVEILESDAV
                     RRIVTPEASYSNEERDLFYRALAFMGSRLVAHGVNVIFDATASRREYREFARSLIPSL
                     LEVSIECPLEVCMQRDKKGTYRRGLQGESSTVPGLQTPYEAPISPALAIDTTVVSSDA
                     AAGQILELIRSPLRS"
     gene            14862..16772
                     /locus_tag="PK224_16200"
     CDS             14862..16772
                     /locus_tag="PK224_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HRB17337.1"
                     /translation="MPASLSHIHPDTPMGANLIAGGATFRCWAPHAKSVHVVGDFNHQ
                     GRNEASLLTRDAQGHWRGFIPGVKDRQRYMFYVVGKGSEGLKRDPYARELESPFPSQC
                     IIRHPDFPWHECGYVTPRFHEYVIYQLHVGTFFTPNLPQKGGTFLDVARKLPYLAELG
                     VNALQLMPIQEFQTSFSLGYNGTDYFSPEMDFAVADADLAPYVATVNRLLDAKGLRRY
                     QVKELRGEMNQLKALIDLAHIYGLAVLLDVVYNHAGGDFGDQSLYFFDRQDPAGGQRH
                     SLYFTDVGHAGGLVFDFAKPEVRDFLIQNAKFFLTEYRVDGFRYDQVSVIDHDGAPEG
                     WRFCQDLTSTVHAQRPETLHHAEYWDVNPFIVAPRPVGAGFDTTLTDGLRMAIRDVIG
                     NASAPDERPLNMTALARSLWPEGFNESWRFVQGPENHDIVYRGREQRIARLGDPDHPR
                     SWFARSRARVATGLSFTAPGIPMLFMGQEFLEDKQWADDFVTHRELLLHWAGLDQGDR
                     QMIDHLRFTRELLHLRRRLPALRGQGFRVAHVHDQNRVLTFHRWVEGEGHDLLVVVHL
                     ADFTRVGYRLGFPGEGDWRELFNSDAYENWINAGVAGNSGRVTAGLQPLHGFAYSAAV
                     VLPANSMLIFAR"
     gene            17028..17345
                     /locus_tag="PK224_16205"
     CDS             17028..17345
                     /locus_tag="PK224_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17338.1"
                     /translation="MNMRSVMSITLIWMGMVAPVLGFQTAAEFPVSWVEHVDLRNHLL
                     TFKTEDGQVRMFRVADSVAMRREWLSKGDLVSIEVDLDDQIVNIVKVDRRSEPNRQVS
                     RRK"
     gene            complement(17507..18358)
                     /locus_tag="PK224_16210"
     CDS             complement(17507..18358)
                     /locus_tag="PK224_16210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB17339.1"
                     /translation="MPTTPPVTFHAPRLVQAPAPSPSSPNHRYSLGQRCRSLYHALWF
                     GLSERIRWSRKPYHEVPVGRLTDLETWQSACIESLQAHYGICFEFRYGRHTSLANYAY
                     LDLLDQAWAAADRPVPTGGLVTDVGCANFWYAQTLHRFFRPAKLTGVDVEGYRLYPTG
                     YSRYDAAVGYVADLPQTSFVAADYCEMDEQVDIITAWFSFVTPAPVLAWRLPLTVFSP
                     ERLFARIARNLAAGGTFFMVNQGTDEEDVAAAYCRQAGLRSQGRWVHPRPLRPRPHPP
                     VASWWTT"
     gene            complement(18483..19313)
                     /locus_tag="PK224_16215"
     CDS             complement(18483..19313)
                     /locus_tag="PK224_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17340.1"
                     /translation="MSVAPFFPQPATGDAFIGRQGLVRNLRGRLADGESVAVIGGPKL
                     GKTSLVRSSLDGLSHHRVIEVDFRQASLPNGDEISGAMLVLDNLDHLPGETIDLLLQQ
                     VSTARPRNMILTGGHRLRALLANPSTLPGLTIRLYPLSVLLDGELRRLVGHDMAAPIA
                     KWTGNHPYLSKLFLHYGETALAEGRQQWQPFLRQLIEEVGKGAERRLLNYLIEYGKPV
                     NPTKAGAETGTEDIKAVADRLVYLGAISRWIRNDEATLFAGCRLLNDFVTGGQSDHAD
                     "
     gene            complement(19387..20211)
                     /locus_tag="PK224_16220"
     CDS             complement(19387..20211)
                     /locus_tag="PK224_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRB17341.1"
                     /translation="MAVRRRGRAWQHRYRAFLAALRRLPFTARLVVGLAAVLLLGAGV
                     NWVYHTIHKPTEMFFALDDALDKHPYETWKEYGALFRTHSTAIMTPDLLAALAQVESA
                     GNPVARTYWRWRLTWNPLEWYRPASSAVGMFQITDATFQEGKRYCIHDHRVVQEGPWN
                     DAHSCWFNSFYSRVLPSHAIELTSALLDRTVANAIGSHRRPRPTFQQKQDLAALIHLC
                     GAGAGHAYVARSFRLAPQQRCGDHSAKLYLDRVNELKRKFARLAAGESLLRFVRPQ"
     gene            complement(20930..21400)
                     /locus_tag="PK224_16225"
     CDS             complement(20930..21400)
                     /locus_tag="PK224_16225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB17342.1"
                     /translation="MPQHPDAGRVELTDWATTGSAIRAIRETVFIHEQGVPVELEWDG
                     LDLSCAHVLAWNDRGEAIGTARMQQNGTIGRMAVLKDWRGRGVGRALLQTLLDLAVRQ
                     GLPRVTLSAQTHALGFYERAGFHVVGKPFMDAGIPHRKMLKELPAAKHQSEPGS"
     gene            21584..21847
                     /locus_tag="PK224_16230"
     CDS             21584..21847
                     /locus_tag="PK224_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17343.1"
                     /translation="MKTQRRIYDVVAGRRIRSGLWHSWCQAGGRAEAQRVFGWSFLRS
                     VWSLLRKVTWGRIGREQESRSSWGHKSIGCSAPRSTAIYGRMN"
     gene            22118..22972
                     /locus_tag="PK224_16235"
     CDS             22118..22972
                     /locus_tag="PK224_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB17344.1"
                     /translation="MKVLIATDGSKYGKWATEWVARMPFAEKPDVSVLHVTDVEALRA
                     PFMFQPVVIGNEPFIQEEIKRIEARGKTAMAEAKAQMASLKLKGKLVSERGAAGSTIL
                     DRAPQRDGLVAIGSRGLDALDRFMLGSVSTQVTLHAPCSVLIVKEEPRPLSRILFAAD
                     GSKASGKALRFLLTKLHPEEREGLEPIDVVVMHAMPFLKYPELKEAGARLVEQCANKL
                     LKAGYVVDEVVQLGKPADEILKVATKKKVDLIVTGAKGMGAVARFLLGSVSTKVVQHS
                     TCSVLVVR"
BASE COUNT         4347 a         6594 c         7468 g         4769 t
ORIGIN      
        1 gcaaccttcg gccaggaacc gatcacgtaa gttctctacg acctgttcgg cggcctgacg
       61 tttcgggacc gagatctgct ccacctggtc cttcgacagg tacttggccg ctagttccgt
      121 catggccggt tcaacgaccg acaacaccgt gacgtgcgcg gattccggaa gaaagcgctg
      181 gcacagaaag cggcaggcgc cttgggagtg tttcgaggca tccgccgcaa agagcacgtg
      241 agagagggct gtgagcggcc gttttaccac caacaccggg caggcagcca aggcgctgac
      301 tttacgggac acactgccca ggaggtaatg ttctgcgtcg ctcaggccgc gcgagccgag
      361 aacgagcaga tcggcttttt tctgcttggc cagcttcgtg atggtatcgg ccacgcggcc
      421 atgggcgagg atcgtgtcga tggctgtgcg aggcttcgtg gtcgcctgct tcaacgcgat
      481 tttcgccaga ctttcgaagc gccgcagaat ctggtcgccg gccttcgtca tggcggcgat
      541 ggcctgtttc gagacagcag cgtgtttacg ggcggcggat ttcaacgatg cgctgtccac
      601 gacatggacc aacgtgacgg agtcgggcgg gcggctggcg accgcttcca gcatctgcac
      661 gctccactcg gcaaactccg atccatccac cgcgatcatc atcttcatgt gtggttctcc
      721 caggttgcag taccgctccg ggatggggtt tgcatcgggt atgcctgaat gcggtctgat
      781 cacgaagcgg taattgcgtg cgatgccagg cggttggcag gtttgccggg cagtcgtcgt
      841 aggcagttgc ggcgttttgc agcagtgggc gggacgatat tggggtaatt ggcatcagtg
      901 agaggggaga tctgaatcgc gacggtccgg aggacatgca cctgtgtacg ttggctgatc
      961 ggtcggcgtt caggcatcac gattgcgtga gaaggtccga aggagggacg cgcaatgttt
     1021 gagagccgag tcattgcggt gatgagtgga atggtgggac tgagtctgtg gctggccgga
     1081 gtggagccgg tcgccggggg agtgacgaca cgtgtggctc cgggcggtga cgcgatcgaa
     1141 ctggagatcc ctaccgaggt cggaacgcag aaagaaaccc tcccgcttta ccacagcggg
     1201 aagatccact atttcagcgc cggcatcggc caggttgagc gtgaggcggc gtaccctccg
     1261 tttcccttga agctggtgtt tacagcaggc gggaagccgt tcgtgacggg ggtggaggtc
     1321 gtgcttcgcc agagcaaggg tgcgacggtg ttgacagttc cccgggagca ggtgacgggc
     1381 ccctggctct ttatcgatgt gccggatggt gcctacgagg tggcggccac gctgggcggc
     1441 cagatacaac aggtgaaggg gattcacgtt cggcgaggac atgtcaccac gcagcatgtt
     1501 cgctgggccg aggaccggag tcctgtcctg cctgcgcagg cagagtgatg ggatagctgc
     1561 ggatagcatg ggtagacaag gaggaggcgg tatggtgaag aagcggaaag ccccggcgag
     1621 acgatcggcc ggtgcgggtc gcagacgcgt ggcattcgaa gacctcacgg tcggcgacat
     1681 tgtgaacaag cgcgtgcagg cggcgcgtct cgacatgaaa ggcgaccgcg tggcgaagtt
     1741 gcttctgaaa gagggaagca acgtgcccgt cgtggatcag gcgaaacagc tcacgggggt
     1801 tgtgagcgaa catgatctgt tgtcggcgct cgatgagggg caggcctgga gtgatcagac
     1861 ggcgaaagat ctcatgaccg aaaatcccta ctcggtgccg ccggaaacca cgctgtctac
     1921 cctgatacat gttctgacgg agagcgatct catgtcggtg ccggtcgtga atgcgggcaa
     1981 tcggctcgtg ggagtggtga cgcgtcgtga tgtggtgcgt gccgcgctcc gtcccggagt
     2041 gaagcgacgg gcgaaaggca ggtaggaatt gacctattgc gtgtgtggag agggggcgac
     2101 gtatgcgccg tatcgacttg atcactcagg cctgtgaccc gaaaacgctg acggtcggcc
     2161 aactgatgca ggatgcgttg tttacctgta cggcacagac ggacgcgctg accatcggtc
     2221 ggctgatgac caaacagaat ttcggcggct tgcccgtagt ggcagaggac ggctctctgg
     2281 tcgggctcgt gaccgaatac gatctcctgc aagccatgat cgaaggccgg gatttgcgca
     2341 aagtgttcgc ttcagaaatc atgacgacgc agcccctggc ggctcgggaa aacatgacgt
     2401 tggaggaagt cgcgaacctg tttcaagacc ggtacgtgac gcgcctgccg gtggtgcggg
     2461 gcaagcagct cgtcggcatc gttgccaggc gggacttgtt gcacgggtat atgaaggcct
     2521 cggcatattg gtcctaggag cctgtccgac attgaccgtt cggctgcggc aaacgatcca
     2581 cgatcatctg cgtcgttctc ggcccgaaaa aatcctcaac gtatttcagc gaatacgctt
     2641 ccggtttttc caggcctgcg gcctcgcatc tggtcgcgcc tccttggcct cgctacgaag
     2701 gccatgtcgg acaggctcct agcatgatgc cggaacaggc tgtgaccgct actgcgcgag
     2761 tagtacagcg ggtcgtcctc atggttgtgt tgttcgctcc cggcacgttg cccggggagg
     2821 cggctatgcc aagacgggcg gccgaaaccc gttccccctg tgccgttcat cacccgagcg
     2881 atgagcgggt cgcctggcat tgccggcgac tacaacgtgg ggaaacactg gagacgttgt
     2941 tcggtcggcg gtgggtcgat gtcgcgcgat tcaaccggat cgatcgccgg catgcgcagc
     3001 cgggacggga gatcaaaata ccgattcggc tcgacgacgt ggcggagttt accccgttgc
     3061 cgggccgcta tcctgccgga gaacaggatc aacaactcat tgtcgtggat ctgacggaac
     3121 agttcatcgg tgcctatgaa tacgggttgt tgagattgtc ctttccgatc gcttccggag
     3181 agccggagaa cgaaactcct gtcggagact atcggattac cgctgcccac cgtactcatc
     3241 gatcctgcct ctataccatc gaatcgacgc ccgtgcccta cccgatgcga tacgcgttgc
     3301 ggttttacgt gaacgcacag ggggtgagtt attggattca cggtcgtgac ttgccgggat
     3361 atccggcctc tcacggatgc atcggtctgg ctgatgagca gatgcagcgc cagtattacg
     3421 gtgtgccgcc taccccggta ctggaggatg ccaagcgatt gtatgagtgg gtgatcggag
     3481 atcgagcgga ttcaggcact ctccttcccc tgtcgaacgg cccgcgcgtg gtcatactcg
     3541 gtagtctcgc gaagcgaggg gatgcgggtg cgtccatgcc ttaaccggtg cgccggtgtt
     3601 gtgtcatggg agaggacagg gtcccgatga tcgcgagcac ggagaagcac gcgcccgcgt
     3661 agaatgtgaa tgccgcgccg agtcgatccc ataagaatcc tgcgaggagg ctggcgatga
     3721 gcatggcgag cccgcaggca aggttgaagc agccgtaggc tgtcccgcgc agatcggtcg
     3781 gtgccacgtc tgcgaccatg gtggccagca ggccttgtgt catccccatg tgaatgcccc
     3841 acagcgccac gcctgccgga atcacgctcc aatgatcgtt catggccagg accagatcgg
     3901 ccgcaaccag caccagcaga ccgagcataa gcagcgtctg gtggttcatt ctgtcggaga
     3961 gcttgccgaa cggataggcc gaggacgcat acaccaggtt catggcgacc atgacgaggg
     4021 gaatgaaagc gatgggaatc ccgccttgtt gggcgcgcag gacgagaaag gcttcgctaa
     4081 agcgcgctaa cgtaaagatg gagccgatcc ccacgaccca ccaataggcc gagcctaagc
     4141 gtgcgaaatt gtcccgggtg atcggattgg ctcttgttgt cgtctggcgg gactcgggct
     4201 cctgtatgcc gaatagcagc agcgcgacgg ccatcattcc cggtatgacg gccacccaga
     4261 agacagcccg gaaatcgttg gcccagagta gcatcaatcc gaccgcgaac agggggccga
     4321 gaaaggcccc caccgtatcg agcgattgac gtaagccgaa cgctgcgcca cggagatgcg
     4381 ccggcgcgat gtccgctacc aacgcatcgc ggggcgcgcc tcgcacgcct tttcccactc
     4441 gatccaataa gcgggcggtc aacaacgttc cgatgtccgg cgcgatcgcg aagagcggtt
     4501 tggtcaatgc acctaaggca tagccgaata aggccagccc cttccgtcta ccgagatagt
     4561 cgctcaatgt cccggaaaag actttgacca cgagtgccag ggactcggcc agaccctcca
     4621 caatgccgac cgcgatcgtg ccggcgccga gtgtcgtgac catgaacagc ggcagcacgc
     4681 tgtgaatcat ttccgaggaa atgtccatca gcaggctgac aacacccagc acccagatgc
     4741 cggtcggcat ccggcggagc gaatggggcg gagatgtgtc catcatggcc gacaagtcga
     4801 ggcgatgaga cggctaccgg gctttctgct gcctgacgga ttatagggaa gccacttagg
     4861 gttgtcatgt cacactcacc aggtcttgga agatgtgatc agttcctgcc cctgtctcca
     4921 gtcgtaacgc acagcatcgg tgagccggcc cgtttcgtct cggtcgaccc acatctccca
     4981 ctgtccatcc tgattggcgt cataccagta aaacaatgga aacaaggcga catcgggttc
     5041 ctcagacgca gggtcgtcat aggaaatccc caagatgtgg cgagccgtgc ggtagtccac
     5101 ctggccgttg ccgcgaagcg agtagtcgcg aaagagcaat ccgatgatcg tgtcggacga
     5161 ttgtgcgact aacaactctt cacctggctc attgggaagt gacgaatcgg cgtgagaagt
     5221 gtccggccag gccggacaca atgtcagcac ggcggacagg catatcgtca gtatcgtcgt
     5281 gttcatggga tccctccctt cactcgcatg agcggtcgag acgagcatga ctctgcatac
     5341 tcaatctttg cgctgctcac ggaagtaggc ccctggatac ctacctagtt agggacggct
     5401 taaaccctaa gtgtgaagta ggcaaggtag ctcgtctgcg cacggatcaa cgatctatgt
     5461 agttgatatg acagcgagcc tctgccatca gaccggtcga tgtgcagccg gtcagtgggg
     5521 aaatgcctgt tgtctgggtg gagtggttgg aatgcgtgaa ccgggatggt gaacgatggc
     5581 ttacatcggg ggacgaggca tgtacaggat gaccgccatg ccgacgaggc agatcactgc
     5641 tcccacgaca tcgaatcgat ccgcctggta ccgtccgtgc cgcagcccca cagcaacgag
     5701 cgtgtgatga acattccgcc agaggctgcg tacgtacggc cgggtcggcg acccttacgt
     5761 tgagggttcg ctgggtacgc tattttcgta gccgcgggcg tggttgtgcg cagggcttcg
     5821 gcctctggag ctcggacgga atccgggtcg cctcatcgag gcaggctgca tccagctgtg
     5881 cttgaaccag ccccagcgcg ccgctcaggt tcgcgcctcg taagtcggcc tggccaagat
     5941 cggcgttgtt cagcagggct cccgtcaaat cggacgcact gagattggct ccgcgcagat
     6001 tggtttcctg gagattggtt tgaaccacga cggcgtggct gatgtcggcc tgactcaggt
     6061 cggcggccac cagattcgcc aattccagat tggcttcgct gagccaggct tgctgcagat
     6121 gggcgttcgg gccgtagatt tcggtgaggt cggctttccg gagatcggcc cgcgcgagtt
     6181 gcgtcccgat gaggacggca tgatgcaggc tggccccgtg cagattggcg gccgacaggt
     6241 tggccttgta cagcacggtc atcgtgagtg tggccgagtc cagcctggct ttcacgaggt
     6301 tggctccctc catgttcgca ttttcaagat tggctcgctg caggttggcg ccgccaagat
     6361 gcgtaaagcg aaaacccgcg ccacgaaggc tggcctcatg gaggtccgca tgcgccagcg
     6421 aggcatcgtc caagtaggcg ctctccagat ctgcttcgac gagggatgcc ccgtcgaggt
     6481 tggcgctgtc catcaaggca ccctgcaggt tggcccggcg taaattggcc tggcgcaaat
     6541 tggtccggcg gaggtcggtt ccggcgaggt cggcgtcggt gagattggcc ttcgacagat
     6601 tcgcgccata aaaatagccg tccaggaggg tggcgttgct gaggtccgcg ccctgcagat
     6661 ccgcatcctc aaaatgagcc cgctccagca gcgcctcccg gaagagcgcg ccttgcaggt
     6721 tggctccgta gaacgcggct tcgtcggcgg cgatgcgatt gagatgggcc ttcaccagca
     6781 gggctttgcg cagatcggcg ccttcaagat tggcatcgtc cagtacagcc tgggagaggt
     6841 ccgcgccttt gaggtgcgcc tgcaccagac tcgcggtgcg aagattcgag cctttcagga
     6901 tcgccccttc aagattgatc cgttcgagat tggctcctac caggcgaagt tgtcgaaggt
     6961 cggtgcggca gagattggcc cggcggccat cgggggagtc ccggtcttcc agccagcggg
     7021 catgggactg gagcaccttg tccaaaattt ttgacgccgg acgggtctgc ttgaaggcgc
     7081 tctcgcaggt attgcctgag gagggtttgg gcgccggctt gggcgcggcg tcagcaccgg
     7141 tcggcaggga gagtaaggcg gccaccaccc aacctgcgac gatggtgctg gaaacagaat
     7201 gctggttcat gaacgggcct ccttcaagaa ggttcggtgc tgtggtgaat ggcatggttc
     7261 cgggagcatc atgtggctca tgtgtgtaaa tcgacctgtt ccgccaactc ggtgagagcc
     7321 ggcgcgatcc ggacgtcgta aggatcggcg cggtcgatcc ggcgcaacgg ggccggcagt
     7381 tgtgtcaacg agagccgggc atatcgtcga acgtcttcca gcgacggagg ggatgcgacg
     7441 cgttgacctc cctgcatcac gtggcccagc aggggctggc cttcccggca gtcgccctcg
     7501 accgtgatga tatcaccgtt caggcgtccg tcggttccga tccggcggta gacctgtttt
     7561 cgacccggcc aggtggcttt gccttcggaa cgtttccggc agggtctgcc ggcatattcc
     7621 tggagtttgt aggcacagtc caggtagggg gcatcggccg atgtggtcat ggccgtgccg
     7681 attgcaaaac tgtcgatcgg tgcctgtcct gtcaccagcg tctggatgcg ttcctcgtca
     7741 agatttccgc tggcgaggat ttgggtggcc gtgaggcctc cgcgatcgag gatgtgccga
     7801 actttgcgcg catggtcggc cagatctccg ctatcgagtc ggacaccgtg gatcgagata
     7861 cctcgtgcgt tcaagcgacc cgccagggag acgaccttct ccgctgccgc ttccgtgtca
     7921 taggtgtcga tgaggaacac gacgttgcgc ggttgcgtct ctgcgatgtg gatgaaggcc
     7981 ttcgcctcat cgagatgagc ctggacaaac gaatgggcca tcgtcccgta gatggggatg
     8041 ccaaagcggg cgccggctga tacgttcgac gtgcccgcga gcccggcgag gtatgcggcg
     8101 cgggcggcga gaaggcccgc ttccgcccca tgcgcgcggc gcaggccgaa atcgatcagg
     8161 ggtttgtcgc cggcaaccaa gaccgaccgg caggccttgg aggccaccat cgtttggaag
     8221 ttcaggagat tcatgacccg tgtttccacg agttgcgcct gtggcagcgg ggcggtaatg
     8281 cggaggatcg gctcgtgtgg aaagaagagg ctcccttccg gcatcgcgtg gacctcgccg
     8341 gtaaagcgca tcgcgcggag cgcgttgagc gacgccggtc tgaatccgcc ggtctgatcg
     8401 agccaggcga tgtcggcgtc tgagaactga aacgcctcca ggtaactcaa gacctgctcc
     8461 agtcctgcgg ccatgaagaa gttgcgttgc ggcggcagtt tccggacaaa gaattcaaag
     8521 acggcgatgt ccgtcattcc ctgggcgaga taggcttgcg ccatcgtgag ttcgtagagg
     8581 tctgtgagca gcgcgtccga ggtgatgttc atgcggccaa ttgctccagg caaaggggaa
     8641 tggcgccggc cagcaccatc gcttcttcgg cgcgccggcc gtcgtcgggg tggagattga
     8701 cggccttgat cccatccatg agcacgcaca catcgtaacc gagcgcccgc gcatccttca
     8761 ccgtgttcag cacacagtaa tcggtcgcca accccccgat gaacaggcgg cggaccttca
     8821 gggcgcgtaa atgacgatcg agtggcgtgt cctgtaatgt ggaataggct tctttgtcct
     8881 gatcgatcgc tttgtagatt gtgaccgccg agacgggagg ctggaagttc ggtggcggca
     8941 aggagccggg cgatcctgcg atacagtgga ccggccaagg cccgccttgc tcccgaaacg
     9001 aacagtggtc gggcggatgc cagtcgcgag ttatgaaaat cggtagccct cgagcatgaa
     9061 atcgtgccac gtacctagtc aggatcggga cgatcctgtc gccatcttgt atgcccaatg
     9121 cgccgcccgg aaggaaatcc cgttgcacgt cgaccaccac taaggcgtca tgaggggtga
     9181 ggtaagtcat tgagcatgtc ccgtccatac ggcatcggct cgcagccggc ggggcgctgc
     9241 gtcattttcc ctgctgcacg agcaggcgga tgtaggccag cacgtccagc ctctccgttt
     9301 cggtcagttg cccctgccag gaatgcatcg cgctgaagac gatgccgtgc tcgatcgttc
     9361 ggagcaactc ctcgtcggac ttgaggaagg acttgaaccg gtggaagttg gccggcggga
     9421 ccgtcaatgc ggcggcatcg ggtccgtcgc cccatccgcc acggccatgg caagcgaggc
     9481 aatgacgctc gtagacagcc ttccctctag tgacatccgg cgggaagtcc tgtgccgtcg
     9541 ccgggccaca ggcggccgcg agggtcacga caagcgagac gacgaaggaa taccgggtgc
     9601 cggtggtcat gacgatctgc atgagtctct gtgtctcctt cagcttttct tggtcgtgtg
     9661 cgcgatgaac gacgaaaagt gttgctgcag tttggtgctg ccgcaggcgg gacattgcac
     9721 ctcacctttt tcatgttgct tcagcgtcac gatgatcagc gattcttttc cgcagtccag
     9781 acaggtatag tcatacatgg gcatagggtc ctcgttctgt ttaccccgtg atcttttccg
     9841 gctcttcttc gccttctccc caattcgcga caatctgcgc catctcctcc aggcgcgcgg
     9901 cggttcgacc gtaaatgctc tcaggcggga aggttccttc cggtccgcgt tccccggcgg
     9961 ggacgccgat gagcagctca agcgcctgtt cgacgtggtc cacgccatag acggcaaact
    10021 ttccctcttc cacggccgcg acgacttccc ggttcagtgc caggtgtttc gtgtttcgag
    10081 cggggatgat caccccttgc gtgcctgtca ggccgcgccg cttgcatgac tcaaagaacc
    10141 cttcgatttt ctcgttgacc ccgccgatgg gttggatttc accgagttgg ttcaccgagc
    10201 cggtgacggc cagcgattgc cggacgggaa ggtcggcgag gcttgagagg acggccgtga
    10261 gttcggccac ggcggcactg tctccctcga tttcggagta ggtctgttcg aaggtgaggg
    10321 tggcgctgag cgcgaacggc tgcatccccg cgaatttgcc cgccaggaat ccggccagcg
    10381 tcatgacccc cttgctgtgg atgtttcccg cgagctcgac ctcgcgttga atatcgatca
    10441 cgcccttggt gccgacatag gtgcgggcgg tgatcctggt gggacggccg aaggcatagt
    10501 cgcccagctc atagacggac aacccgttga cctgaccgac gacctcgccc tgcagatcca
    10561 ccatcaaggt cccttcgcgg atttcatcct ggatccattg ctcaggtagg tcggcacggt
    10621 gtctctggtg cgccagggcc gattcgacat ccgcgcgcgt cacgaaggag tgcccgtctt
    10681 tcctggccca atacccggct tcgcgaatga ggtcgctgac caggctgaat cgcaaggaca
    10741 gccgatcgtg ccggtcggca tagcgaaacc cctggcggat gacttccgcc acggcgtcgg
    10801 cgccgaaatg cggcagtcct tcctctttgc ataatttggc gacgaagcgc gcatattgcc
    10861 gctcctgccg ctcatcgtgc gggacctcca catcgaaatc ggctttcacc ttgaagagtt
    10921 tcgagaaatc ctcttcgtag gcctgcagca ggtggtagat tcccgctgtg ccgaccagga
    10981 tgactttgac cgagacggga atcggttcgg gccgcaggcc cgcggtggcg aatccataga
    11041 actcggccgg gtcctcgatg gtgacggcgc cggtcttgat gacgcgcttc aacgcgtccc
    11101 aggaaaacgg ctggcggagg agatccagcg cctgcagaat caagtagccg ccgttggcct
    11161 gcaacatggc cccggcccgg atttcggtga aatcggtata catcactccg aggtgcgccc
    11221 ggcgctcgat ttttccgatg aggttgctgt aggtcgggtg tggttcgtcc acaaccggtg
    11281 ctccggcagt ggcttcccgg gccacgatga ggttgacggc gaaccgggtc atgtccgggc
    11341 gacgggtttg ttccagcccg gggatcggca gcaccggacc gctgtgaggc aggaagtctt
    11401 tgtattggtg aatgatgtcg tgatggaccc gctgcaggta cgtcgtcacg ggtcgtagat
    11461 cttgataggc ccgctgcagc gtttcatagg cgcctttcat gacgttagcg acgatctggt
    11521 ggtcgaggtg atgcagcgcc tgctcggcct cccgttcgag ggtgtgcatc cggacgtgaa
    11581 actcccgcag gtcgctctcg agcgactgtc gtttttccgt gagttcctgc tgcacctgtt
    11641 cagagagtgc ttccatttcc ttttctgtca tcgggcggtc gtctttcagg gggaccagcc
    11701 cgaagccgac cggcgtttct tcgaacccga atccgcgctg gtgacagagc gcggtgaggt
    11761 cgtggaagag ggccttcttt tttccttccg tctcctcgat gatcttcgcc ttggcatcga
    11821 ggtacttctt gccttcgaag gccgcgggaa tgtcccggcg gaggccctcg atgaaggtgc
    11881 tcatctcgcg tttaaacgac gcgccttgcc cggccggaaa cgaaaggcag gtcgggcgcg
    11941 acgcgtcctg aaagttgtgg acgtaacacc agtcgaatgg agccggtgcc gtttgggcca
    12001 gccgcttcac catttgcctg acgagtgtgc ccttgccagt cccgaccggc ccggagacga
    12061 acatgttgaa gccggggctc ttcatttgaa ggccgaagtc caaggcctcg accgcccgct
    12121 cttgcccgat cgtgtcatcg agcggttcga cctcgctcgt atcatcgaat cccaggcggc
    12181 cggggtccac ctgcggagcc aacaggctcg cgggaagctt ctgtttgtct gacatcgtcc
    12241 ggactcctac acttccagcg cgtggggtgg ttgaacgatg gtggcgtgta ggcccttttc
    12301 gcccggttcg ctttcgaaga ggacctcctg tccgtcctcc atgtcgttga atgggatgcc
    12361 cttcacggca ttcttatgga aatacacttc gccgccgccg tccttcagga tgaagccgta
    12421 gccgaggtga gggaatactt tgctcaccac accgcggagt tggggcagcg gttcgatgcg
    12481 cacaactttg tcggcccgct tctcgcgata tttccgcagt tcgatcgcca cggcgtcgaa
    12541 ggcggcccgg atcgcttcct cgaaagtttt gtcctccttg cgggaggtga tggtgtgacg
    12601 ggtcggcacc gtgaccagca caagcgcttc tgccacgttg tccagttttt tatgatggcg
    12661 attcttcgtc agggtgacgc gcccatggat gatgtcgtcg tggccacgct gcaaggtggc
    12721 catccgctct tcgatctcgg ttttccagcg cggggtcatg gcgatattgc ggctctcaac
    12781 ttccaggttc atgcggcctc ctctcgtctc agatgaacag tctgtgcacc tgctgtctca
    12841 gcaaagcaca tgcccttgcc gcagatggga acgcggggcg gggagggagc tgaaccggct
    12901 gaaagtgggg catttgcgcg acttcagcag acgggatgat ggtggccttt cggggcactg
    12961 gcgcataatt cctcactgcc tcaggaagcg ctgaagctca atgcgccagt tgttggttcc
    13021 gttgcgggct aaagcggcat atttccggga acatgtcttg cgtcggacag cggtatgccg
    13081 gtgttgaaga aatctgccct ggagacctac ctcaaggccc ggttcgggcc gagtgcggaa
    13141 ttgctcgcct atggtccgat cggcaaggag acgaacggcg cccggtataa gcaatatggc
    13201 tatggcgccc ctgtccggtt gactttccgc caggggggca agacacgccg ggtggtgttg
    13261 ggcacgatga gtcccggtcc attcggccat gaacatccgg ccgaccgggc tcaggcgatg
    13321 ttgtgggact atgactgcta cggccgcctg ccgggacata tcgacgcgct ggatgtgggg
    13381 gccttcaccg cgcggggcga attgatgtcc gtatcggccg cgaaggaatt ctttttactc
    13441 accgaatggt cagagggagc gacctatcac aaggatctcg aacgcatggc aggggcgacc
    13501 aagccgactg cgctcgaccg gaagcggacc ggagtgttgg ccggatatct ggccgacatt
    13561 caccgggtga aacagaccga tccgcaactc tatcggcggc ggttgcggga attgctcggc
    13621 catggcgaat gtatcatggg cctcaccgat agttaccccg atcggtttgc cttcatcacg
    13681 gaagaccttc tgtcgcgaat cgaggtggcg tgcaatgtgt ggcgatggcg actgcgcagc
    13741 cggcatgcgc gcctgtcgca ggtgcatggg gatttccatc cctggaatgt gctgtttcgc
    13801 cacgggacgg atttttccgt cttggaccgg tcacgcgggg agtggggtga gccggcggac
    13861 gacgtgacgt cgatgtcgat caactatctg ttcttctcac tgtgccggca tgggacgttg
    13921 aagggcgcgc tggaagtggc cttccattca ttctgggaga cctacctcgc caggagccgc
    13981 gatcacgagg tgttggaggc gacggcgccg tttttcgcgt ttcgcgggtt ggtgctggcg
    14041 agtccgctct ggtatccgaa actgaccgtg actgtgcggc ggaaaatctt ccggttcatc
    14101 gagaatgtcc tggcgacggc gcgcttcgac ccggcggatg tgaaccgcta ttgtgaataa
    14161 gggatgtgat gactcctttg ccatttgccc tgtggctgac cggtcttccc gcttccggga
    14221 aaagctcgat tgtcgcgcgg ttgttaccca ggctcgaatc gtggggagtg ccggtcgaaa
    14281 tcctggagtc cgatgcggtc cgccgcattg tgacccccga ggccagctat tccaacgaag
    14341 aacgtgacct gttctatcgc gccctggcgt tcatgggctc gcgattggtt gcgcatgggg
    14401 tgaatgtgat cttcgacgcg acggcaagcc ggagggagta tcgggagttt gcgcgcagct
    14461 tgattcccag cctgttggaa gtctccatcg agtgtccgct ggaggtctgc atgcagcgtg
    14521 ataagaaggg gacctaccgg cgcggcctgc agggcgagtc gtccactgtg ccagggttgc
    14581 agacacccta cgaagcaccg atctcgccgg ccctggctat cgacaccacg gtcgtctctt
    14641 cggacgctgc agccggacag attctcgaat tgatccgatc ccctcttcgg tcgtgaggtg
    14701 tttcttctgt ctccccgcgc cccggcactc agtcttctac agtttctcat cgcgataggc
    14761 gatcagtcat ctgccgccgg ctcttcagct gccgcttgcc gagttgagcg gcctccgata
    14821 ggatggggct gttccatcga ttgcttaccg tgagggcatc catgcccgcg tcactcagcc
    14881 acatacatcc cgataccccg atgggggcca atctgattgc cggcggcgcg acgttccgtt
    14941 gctgggctcc gcatgccaag tccgtccacg tggtcggcga tttcaaccat cagggacgta
    15001 acgaagcgag tttgctgacg cgggacgcgc aggggcattg gcgtgggttc attccggggg
    15061 tgaaagatcg ccagcgctac atgttctacg tggtcggcaa ggggagtgag gggctcaagc
    15121 gggatcccta tgcccgcgag ttggagtcgc ccttccccag ccaatgcatc attcgtcacc
    15181 cggactttcc ctggcacgag tgcggctatg tcacgccgcg ttttcacgaa tacgtcatct
    15241 atcagctcca tgtggggaca ttcttcaccc cgaatctccc gcaaaaaggc ggcacctttc
    15301 tcgatgtggc tcgtaagctg ccctatctcg ctgagcttgg cgtgaacgcg ctgcaattga
    15361 tgcccattca ggagtttcag accagcttca gcctgggcta caacggcacc gactattttt
    15421 ctcccgaaat ggatttcgcc gtggcggatg ccgatctggc tccctatgtg gcgacggtca
    15481 accggctgct ggacgcgaag gggttgcgcc ggtatcaggt gaaggagctg cggggggaaa
    15541 tgaatcagct caaggcgctg atcgatctcg cccatatcta tggtttggcg gtgctgctcg
    15601 atgtggtcta caaccatgcg ggcggagact tcggggatca gagcctgtat ttcttcgacc
    15661 ggcaggatcc ggccggcggt cagcgtcact cactgtactt caccgatgta ggccatgccg
    15721 gcggtcttgt gttcgacttt gccaagcctg aggttcgcga cttcctgatt cagaacgcca
    15781 aattcttcct cactgaatac cgtgtcgatg gattccggta cgaccaggtg agcgtgatcg
    15841 atcacgacgg cgcgcccgag ggctggcgtt tttgccagga cctgacctcg acggtgcatg
    15901 cacagcggcc ggagacgttg caccatgcgg aatattggga tgtgaatccg ttcatcgtcg
    15961 cgccgcggcc ggtcggggcc gggttcgaca ccacgttgac cgacggcctg cggatggcga
    16021 tccgcgatgt catcggcaac gcgagcgcac ccgacgagcg accgctgaat atgacggcgt
    16081 tagcgagaag tttgtggccc gaggggttca acgagtcctg gcggtttgtg caggggccgg
    16141 agaaccacga catcgtctac cggggacggg aacagcgcat cgcccggctt ggcgatccgg
    16201 accacccccg atcctggttt gcgcgcagcc gtgcgcgggt cgcgaccggc ctgagcttca
    16261 ctgctcccgg gattcccatg ctgttcatgg gacaggaatt tctggaggac aagcagtggg
    16321 cggacgactt cgtgacacac cgcgagttgc ttctccattg ggccggtctc gatcagggcg
    16381 accggcagat gatcgaccat ctgcggttca cgcgggaact gttgcattta cgccgccgat
    16441 tgccggcgct gcgtggacaa gggtttcgcg tggcgcatgt gcacgatcag aatcgggtcc
    16501 tgacgtttca tcgctgggtg gaaggcgaag ggcatgacct gttagtggtg gtgcacctgg
    16561 ccgacttcac ccgcgtcggg tatcgactcg gattcccagg cgagggtgac tggcgcgaac
    16621 tgttcaacag cgatgcctat gaaaactgga tcaatgccgg cgtggccggc aacagcgggc
    16681 gcgtgaccgc cggtctgcaa cccctgcacg gctttgccta ttcggccgcg gtggtgctcc
    16741 ccgccaatag tatgctgata tttgcgcgtt agtgtccccc ggattccctt ccccgcatct
    16801 ccatgtggtc tctttcccga gcctacgggc ctgttcttaa cgaacccgtc gcctttgcgc
    16861 gctacaagag ttcttcgtag agcggacgac cgcaggctga ggtcttatct ccgctacaaa
    16921 atggcagcag gagcgacaga ctgccggtgc gatggggcgt agagtctcga tgtgcgcgac
    16981 acgccgttca tgttcgaccg catacctgca atcgagagga ggtcacgatg aacatgcgta
    17041 gtgtaatgtc tatcacgctg atctggatgg gaatggtcgc cccggttctc ggatttcaga
    17101 ccgccgcaga gtttccagtg agctgggtcg agcacgtgga tcttcgcaat cacctactga
    17161 ccttcaagac cgaggacggt caggtgcgga tgttccgggt ggccgattcg gtggcgatgc
    17221 ggcgtgagtg gctgagtaaa ggggatctcg tcagcatcga agtcgatctc gacgaccaga
    17281 tcgtgaacat cgtgaaagtg gatcggagat ccgaacccaa tcgacaggtg tcacgtcgaa
    17341 aatgaggagt gacagatttg tgtgagtgct caggatcgcc tcgcaggatg cgcaagaggg
    17401 cggatcagca aggccgcagc aagcgaaggg gcgaatcgta ctcgcgccgt acggtgagcc
    17461 gctgagcgcc gcgagaacgc cgctggcgga ctttttccgc atcctgctag gtcgtccacc
    17521 aggaggcgac cggaggatgg ggccgcggac gcaatggccg cggatgaacc cagcggcctt
    17581 gtgaacgaag ccctgcctgt cggcagtatg cggcagcgac atcctcttca tctgtgcctt
    17641 gattcaccat gaagaaggtt cctcctgccg ccaggttacg cgcgatacgg gcaaagagcc
    17701 gttccggaga aaacacggtc aacgggagcc gccaggctag aacgggtgcc ggcgtgacaa
    17761 acgaaaacca ggccgtgatg atatcgacct gttcgtccat ctcgcagtag tcggcggcca
    17821 caaatgaggt ctgcggaaga tctgcgacgt atccgacggc cgcgtcgtag cggctgtagc
    17881 cggtcggata gaggcggtaa ccctcgacat ctacgccggt cagtttcgcc ggtcggaaaa
    17941 accggtgcag cgtctgcgca taccagaagt tggcacagcc cacatccgtg acaagaccgc
    18001 ccgtgggaac gggccggtcc gctgccgccc aggcctggtc cagcagatcg agataggcgt
    18061 agttcgctag ggacgtgtgt cgtccgtagc ggaactcgaa acagatacca tagtgggcct
    18121 gcagggattc gatacaagcc gactgccagg tctctagatc tgtgagacgg ccgaccggca
    18181 cttcatggta aggctttctc gaccagcgta tccgttccga gaggccgaac cacagcgcat
    18241 gatagagcga ccggcaccgt tgtccgagag aataacgatg gttgggcgat gaaggactgg
    18301 gggcaggagc ctgaaccagg cgtggagcat ggaaagtgac tggaggagtg gtcggcatag
    18361 ggagaatgtt tcatcaccat gtcggctttt ccaatgaaga actgaagggg ggcggatggt
    18421 aatggctagc catcatccca tcggtcgttc tgtaaacatt cgcggttcgg cacagcatgc
    18481 gcttaatctg cgtggtcaga ctggccgccg gtcacgaagt cattgagcag ccgacaaccg
    18541 gcaaaaagcg tggcctcgtc atttctgatc cagcggctga tggctcccag gtagacgagg
    18601 cggtctgcga ccgccttgat atcttcggtg ccggtctcag ctcctgcctt cgtagggttc
    18661 acaggtttcc cgtactcgat cagatagttc aataatcgtc gttctgctcc cttgcctacc
    18721 tcctctatca gctgtcgaag gaacggctgc cattgctgtc ggccttcagc aagagccgtt
    18781 tctccgtagt gcaggaagag tttgctcaag tacggatggt tgcccgtcca cttcgcgatg
    18841 ggtgccgcca tatcatggcc gaccaaccgg cggagttctc catcgagcag gacagagagt
    18901 ggatagaggc gaatggtcaa cccgggtaac gttgaagggt tggccaacag tgctctcaac
    18961 cggtggccgc cggtgagaat catgttccgc gggcgggcag tcgaaacttg ctggagcagc
    19021 aggtcgatcg tttcgcctgg cagatggtcg aggttgtcca gaacgagcat tgcgccggat
    19081 atctcatctc catttggcag tgatgcttgg cggaaatcca cttcgatcac ccggtggtgg
    19141 gatagcccat ccagcgagct tcgtaccagc gacgtcttgc cgagcttcgg tccgccgatc
    19201 accgccacgg attcgccgtc cgctaggcgt ccgcgcaggt ttcggacaag cccttggcgt
    19261 ccgatgaacg catcgccggt ggccggctgt ggaaagaagg gtgcgacgga cataggccac
    19321 tttaccacgc atggccatga tacgactcat cgctcgtgcg atcggggcaa tctcggtcga
    19381 gactcgttac tgagggcgca caaaccggag caggctctcg cctgcggcca gccgcgcaaa
    19441 tttccgcttc aactcattca ctcgatcgag atagagcttc gcactgtgat cgccgcaccg
    19501 ttgctgcggg gccagtcgaa agctacgggc gacgtacgcg tggccggcgc cggctccgca
    19561 gagatgaatc agggcggcca gatcctgctt ctgctggaac gtggggcgag gccttcgatg
    19621 cgaaccgatt gcgttcgcca cggtgcggtc gagcagggcg gaggtcaact cgatggcatg
    19681 gctcggcagg acgcggctgt aaaaggaatt gaaccagcag gagtgagcat cgttccaggg
    19741 gccctcctgg acgacacggt ggtcatgaat gcagtaccgc tttccttcct gaaacgtcgc
    19801 gtcggtgatc tgaaacatgc cgacggcgct cgatgccggg cgataccatt ccagcggatt
    19861 ccaggtgagt cgccagcgcc agtaggtacg cgcgaccgga ttgcctgcgc tttcgacctg
    19921 cgccagcgcg gccagcagat ccggcgtcat gatagcagtg gaatgtgtac ggaagagcgc
    19981 accgtattct ttccacgttt cgtaggggtg cttatcgagc gcgtcgtcga gcgcaaagaa
    20041 catttccgtc ggcttgtgga tggtgtggta gacccagttc acacctgcgc caagcaggag
    20101 aacggcagcc aggccgacca caagcctggc cgtgaacggc aagcgccgca gggcggcgag
    20161 gaaggctcga tatctgtgtt gccaggcgcg cccgcgccgg cgaacggcca caatcgtgct
    20221 cctgcgaccg cgccttctgc gacggatcac agcctgcgga tgcgaacggg aaggcatggg
    20281 gtcactataa ctcaaaaaga ggaggcggag gattggtcat cttcttgctc gcggaaggtg
    20341 tgatcagacc atgctcagcc gatgtctggg atggagttca tgccagccag gtcgccgatc
    20401 aaagcaggaa gtgatatcct caccgacgaa gaataataat tgtgacgtgt gcatactccg
    20461 tcgcgtagcg cggtatgtgc gaggaaggac taggcggatt ttgcactgcg ggatatgcaa
    20521 tatctgagga taacatgccg gcaccgattt gcctttgggc tgaaggcact gaccatgatc
    20581 acgctgatca gtttcgcgac ggtttcgcat gccggcacga aacgaccgcc tgagtcccat
    20641 cgctcagggg tgaagcctca aaagaatgga ttctgttctg cggaagctcc aatcaagggc
    20701 aatttcacga cccatagcgg tgagcgttgc atctatcaca tgcccggtca gcgtttttat
    20761 gagacgacaa ttcccgagcg gtgttacgct aacgaagccg aggccattcg ggatggttgt
    20821 cgtaagtcga aggtgtgaga tggtgacgtg cccgccaatc cctctcttgt gtccccgcga
    20881 aattgcatct accggtcaac gatgatcttc tatcgttcac tcttcgcggt tatgatcctg
    20941 gctcagactg gtgtttcgct gcagggagct ctttcaacat cttccgatgc gggatgccgg
    21001 cgtccatgaa gggtttgccg actacgtgga acccggcccg ttcataaaat ccaagcgcat
    21061 gggtctgtgc ggacagggtc acgcgcggga gcccttgcct aaccgccaga tccagcagtg
    21121 tctggagaag ggcccgtccg acgccgcgcc cgcgccagtc cttgaggact gccatcctgc
    21181 cgatggttcc attctgttgc atgcgcgccg tgccgatggc ctctccgcgg tcgttccacg
    21241 ccagcacatg ggcgcaagat aaatcgagcc cgtcccactc tagttccacc ggcactccct
    21301 gctcgtggat aaacaccgtt tcgcgaatcg ccctgatggc cgatcctgtg gtagcccagt
    21361 cggtgagttc tacccgtcct gcgtcgggat gttgcggcat gattgtctga cctcctcaat
    21421 ctgcgcggtc ttctgcatcc gactccggtg agacggcgcg tctcaaatgt aattctcttt
    21481 tggaggatcg gcaaccacat ggtaccgttc atcatgatgg tctgcctgtg aaacgacgca
    21541 ggcggacgcc gagtggctcc ggttcaccga tgggaggtca tccgtgaaaa ctcaacgtcg
    21601 aatctatgat gtcgtggcag ggcggaggat ccggtccgga ttgtggcatt cctggtgtca
    21661 ggccggtgga agggctgaag cccagcgggt gttcggctgg tcgtttctgc gctcggtgtg
    21721 gtcgttgctt cgcaaggtga cctggggccg gattggtcgt gagcaggaga gtcgctcatc
    21781 ctggggacac aaatcgatcg gttgttccgc gccgcgatcc acggccatct atggtcgcat
    21841 gaactgaccg gcggtattcc gttcggttga ttgccattca gcccgctcgt tgttctgcct
    21901 tctgtgtctc cgtccttttt tcggttcttc gctcaaagca taggcatctg tccccgacgg
    21961 caaccttcct gtgaccttcc acagggggaa gaagggctgc cttatttctg cggaaagaag
    22021 gtattgaccc gctgatccgt tgaggcgaag atgcggcagc gttggcacgg agcctgctac
    22081 tcaagtctgt aacggattga acgatgaggg gcgacctatg aaagtcttga ttgcgaccga
    22141 tggatcgaaa tacgggaagt gggcgacgga atgggtggcg cggatgccgt tcgccgagaa
    22201 gccggatgtg agcgtcctgc acgtgacgga tgtcgaggcc ctgcgcgcgc cgttcatgtt
    22261 tcagccggtg gtgatcggca atgagccgtt tattcaggaa gaaatcaagc ggattgaagc
    22321 ccgtggaaag acggccatgg ccgaggccaa ggctcagatg gcgtcgctca agttaaaagg
    22381 caagctggtg tctgagcgcg gggcagccgg ctcgaccatt ctcgatcgtg cgccgcagcg
    22441 tgacgggttg gtggcaatcg ggagtcgggg gttggatgcg ctcgaccggt tcatgctcgg
    22501 gagtgtctcg acccaggtca cgttgcatgc accctgctcg gtcctcatcg taaaggaaga
    22561 accgcgtccg ctgagccgga ttctgttcgc agccgacggg tccaaggcct caggcaaggc
    22621 cttgcggttc ctgctgacca agctgcaccc tgaagaacgg gaaggcctgg agccgatcga
    22681 tgtggtggtg atgcacgcca tgccgttcct gaagtatccg gaactcaaag aagccggcgc
    22741 ccgcttggtc gagcaatgcg cgaacaagct gctgaaggcc gggtatgtgg tcgatgaagt
    22801 ggtccagctc ggaaaaccgg ccgatgagat cttgaaagtg gcgacgaaga agaaagtcga
    22861 tttgatcgtg accggggcca aaggcatggg ggccgtcgcc aggttcctgc tgggcagtgt
    22921 ctccaccaag gttgtgcagc acagtacgtg ctcggtgctc gtggtccgtt aggcaggctg
    22981 ttgacaaagg ccgccagcgg cgttctttcg gcgttcaaca gcctgcggca ggttctggga
    23041 acgtctgggc tctgctggtt cctcccgggg caactaaccc gcattattca tgacggttcg
    23101 tcgtgaaatc gccgagtcac gtggcgagac cggcgcgcgg agccgggagg acccgaggcg
    23161 tactcgtgca gtacgttg
//
LOCUS       DAORRQ010000062        22947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_135_length_22947_cov_8.265464, whole genome shotgun sequence.
ACCESSION   DAORRQ010000062 DAORRQ010000000
VERSION     DAORRQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22947
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135_length_22947_cov_8.265464"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK224_16240"
     CDS             <1..602
                     /locus_tag="PK224_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRB17345.1"
                     /translation="PSQMGLEDLAMMSAQPGVTVLYPSDALSMYKLVEAAAAHKGMVY
                     LRAGRPKTPVIYGVDEQFRIGGSKVVRQSAADQLTIVAAGVTLFEALKAHDQLKAAGI
                     TTRVVDLYSIVPVDRATLLDCARATGGRFLTVEDHYAHGGIGDAVLSALASEGVRVHK
                     LAVREIPRSGKPDELVDHFGIGVRSIVEAAKEIVKTASR"
     gene            520..1299
                     /locus_tag="PK224_16245"
     CDS             520..1299
                     /locus_tag="PK224_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRB17346.1"
                     /translation="MSSSIILELEYARLSKRPKKSSKQPPAEQSGPAFPAGLNQIPLL
                     QILSAQDRQKVLGEMMETRYGKGQYIFREGDPTEYFHIVKEGSVKCVKSSLDGKECTL
                     KVLMPGDLFCCDASAFDGASHPGTAQPMGDVSVLRMKKEAYFKMLRANPDAAMEVIRY
                     LGNRLNEAQEKAKVLALDRADQRLASLLVNLAERSGVKEPNGIRVSVRLTRQDMANMV
                     GVTTETAIRIMARFKKERLVSGTAARLIIRDLPKLRILAST"
     gene            1474..2445
                     /locus_tag="PK224_16250"
     CDS             1474..2445
                     /locus_tag="PK224_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRB17347.1"
                     /translation="MQEQAGDNNQGRGRCKTASLLGLTLGMVASMALSVQAKTHDIQM
                     TAVETDIVIDGGGEKYAAWTFNGQFPGPVVRVTEGDTINFTLTNSTTNKNPHAMDFHA
                     AEIDFLKNYRAVNTGESISFTFVAKKPGVFFYHCGAPPMIQHVARGMFGAVIVDPKDA
                     KVWPKADREYVLVQSEYFKNPNDVQAMFDRKYDGMMFNGGIFKYHPFVTGGGKLDAKP
                     GERVRIYFVNAGPNEFSSFHPIGEIWDNVYESGNPANNLKGLQTYVVGPGSAATFDVV
                     VESAGAYPLVTHSLTGALRGAIAVLLAGPDAKPAPLMPMVPWELPAK"
     gene            2560..2907
                     /locus_tag="PK224_16255"
     CDS             2560..2907
                     /locus_tag="PK224_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17348.1"
                     /translation="MPFRGMTRCCLLLSVGCLASPVNADTSHERVALFLSGQDCPSFR
                     PSLAAELAQVPGVTRVEPDSVPDHVLIDVVSGQVTSEDLLAAARRRLIPGMRCRIEIM
                     QSCISAGPAPLPR"
     gene            2987..3598
                     /locus_tag="PK224_16260"
     CDS             2987..3598
                     /locus_tag="PK224_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17349.1"
                     /translation="MAHKWTGLLCGAIAIVGWCLASAASLSAQEAMLEFPIRPFNVDV
                     MLRQQTFAPDDREIGVQLGITALRYGNVEVRTIYQYFSIHSQEFRTDQHSLFLNPRWN
                     NFIDILDFPTHKPISRLLRHILFGPLEDRAVPYVGLLAGGVLPGPGNHAPGHLIGGQL
                     GVRFPVARSLSVDISVQYSQYGVDFRGEAGQAQQWVFLTGVRF"
     gene            3598..4629
                     /locus_tag="PK224_16265"
     CDS             3598..4629
                     /locus_tag="PK224_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRB17350.1"
                     /translation="MRASRVRVGWMAVLCLCLPGCGLLIDAVEHVWPVSGNKVDIICE
                     RERVQVGLAMEPFRPFVFPTIWTDEGARVTGLDVELVRAMTDELSRRCGRAVTPVLHL
                     VRFRDLFRLLNEGHLDWFMSAVAINTPAPARAGVAYSLPYFYGGGISGITTSPAVLER
                     VRANVREQAIHPTPDRLAATSHALDGLTIAVQDETSAYYYAEANLSPHRLILCDSLPA
                     AFEYADSQAEPRVDVILGAQPVLEYMVKRVRKDWSPLARENGRPLFLTKADYGVVVAE
                     ESYHLRWLLNDLLLKLDESGRLREMRTRWLDEEYAFPRRASLEGLSFDVEQMAAHYAQ
                     GTCRLKVEP"
     gene            4652..5119
                     /locus_tag="PK224_16270"
     CDS             4652..5119
                     /locus_tag="PK224_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17351.1"
                     /translation="MRTHAFCILAAAVLLVIAQPSMVGAVERAEAEETARLLAKLLQS
                     GRMVIERNQTLIDDPHKGEKGFTPELFEQQLVREFLAKTGIDLTALPAAPVSILIPPL
                     AKELLPALVQASREVVRDAQVVINHAASAIRISSRRRMGVRPRLASPSRRTCA"
     gene            5056..5412
                     /locus_tag="PK224_16275"
     CDS             5056..5412
                     /locus_tag="PK224_16275"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB17352.1"
                     /translation="MPATYGSQASARFSKSAHVRLKQTAIEPRNPKNEPDEYEASVLK
                     WLSARPRAEAYVSELTEEGRMLRVVMPIYYAKDCLACHGEPKGDLDISGYPKEGHKEG
                     DLAGAITVTAPLTIRK"
     gene            complement(5483..5803)
                     /locus_tag="PK224_16280"
     CDS             complement(5483..5803)
                     /locus_tag="PK224_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB17353.1"
                     /translation="MKVLTLADFQQFSSEKMKKNNLFQTPRFFCDIYCFEPGQEQKGH
                     IHGEQDKVYIVLEGEGTFQVGSEKQVLRPGQGTMAPAGDEHGVKNHTQQRLKVLVFVA
                     PNQA"
     gene            complement(5821..6723)
                     /locus_tag="PK224_16285"
     CDS             complement(5821..6723)
                     /locus_tag="PK224_16285"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRB17354.1"
                     /translation="MPLKFALYPGCAAKGATPELYQSTMAIVGRLGIEVVELAASSCC
                     GAGVIGEADPDVALALNARTFAQAERLGLDIMTICGTCQGVMSAANRRLKNEPGTLER
                     VNTILAQDGISYGGGVQVKHLLWIAVRDIGLTRVGETVHSPFRNFRIAPFYGCYMLRP
                     SWELGFDDPENPSSLEKIIRAVGGEPVAYAGRTKCCGFPIILEKEAIAVAMAGTNMKD
                     AKDHGADFMVTPCPLCHMSLDIYQERAGQAVKTALNLPILHLPQLLGLAMGLPAQDLG
                     LSRHLISVDRIVTRLHTPDTTHSS"
     assembly_gap    6965..6990
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6991..7290)
                     /locus_tag="PK224_16290"
     CDS             complement(<6991..7290)
                     /locus_tag="PK224_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17355.1"
                     /translation="MAVARTLAFLCMIGISLTLPPRTTAGDQPQPSTTDRVIRDTKEA
                     VEATKQYTLQQKETFQKAVQAELAEMQVKIAELQKKTHAASAEARTEMQKALQDLE"
     gene            7671..8540
                     /locus_tag="PK224_16295"
     CDS             7671..8540
                     /locus_tag="PK224_16295"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HRB17356.1"
                     /translation="MSAELPKATDAASTDALEQHLIERIRKGAIELPLLPQVASRILS
                     MVYDPDAEAAKLAALIHQDQALAAHVIRIANSPAYMTRNPVVSLQHAVSMLGMNLMSE
                     LAFSASIKGSAFKVPGWDDEVKRLWQYSLASGAYAKEIARVRRFNVESAYLCGLLHGI
                     GKPVVLQTLVALAKEQNLTLTQELLHQLLEGYYIQVGLLVAEDWGLPPPVVESIGFHS
                     DYQYAKTAKQECMTTCLAGRLANHFLDPVAFDEAMVREHAVFADLNLYPKDIDALLAL
                     KDKVQAVVEAMPL"
     gene            8540..9958
                     /gene="sthA"
                     /locus_tag="PK224_16300"
     CDS             8540..9958
                     /gene="sthA"
                     /locus_tag="PK224_16300"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HRB17357.1"
                     /translation="MPPNAYDIVVVGSGPAGQKAAIQGAKAGKKVVLIEQEQGIGGNC
                     VYRGTIPSKTLRETALQFERLKRSSEVFEGRLRLDVPMSVLLHRLDEVVKAHECYMAD
                     QLTRNSVTYRHGRARFLSPHEVELETIDGACQALRADTIVLATGSRPRSIPEIPVDHE
                     HVLDSDSILSMIYLPRSLTVLGGGVIACEYASTFALLGVEVTLIDRAQRPLSFMDAEI
                     VEVFQRSIERQGGRFYVGQTVKEVVWDGVSSVVARLANGMAVKSEKMLVALGRQPNIE
                     ELNLEAAGLTLDEKGRIPVNEYGQTPVPHIFAAGDMLGRPPALASQAMEDGRRAVSHA
                     LGLPVGDSLNQVPIGIYTIPEIASIGLDEEQAAARYRGPLVGRARFTEIAKGQITGSC
                     DGLLKLIADPSGERLLGVQIVGEHATELIHLGQMALQDGATIDRFIDSIFSFPTFAEG
                     YRVAALDILGQRRKRQSTTQAA"
     gene            10057..11760
                     /locus_tag="PK224_16305"
     CDS             10057..11760
                     /locus_tag="PK224_16305"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17358.1"
                     /translation="MNRNERRRDEKLHGKQAGGADAPAALLREAQLHHQAGRLDEAER
                     GYRSLLERAPTQPDALHGLGLLNYRRGHLNDALGWLAKACAAGPRNPIYWFNHGVVLQ
                     RAGRTPDAVEAYGQALHWNPRYIEARTNLGNAYKELGRLSDAQAAYEQVLTLNPDLAE
                     AHNNLGVVLKEQGRLDEAADSYRRAIALKPTHAEAQNNLGLVLLEQGRLDDAIRCFER
                     ALQIVSGYGTALYNLGIAWIWREDIPRALRCFAETAQAKHAHGRPVAETTVFRSRLKH
                     DAEQLEYLRESGLLGDEWNPYHEALTRLCEKLEHSATGATGSSNRVLLSPADMQPIAA
                     SYNRLIHIAPCEAIEGGALAADLDSAAVEARYLATNPEVTYIDGLLADEALQRLRQFC
                     WASTIWKKDYENGYIGAFLGDGFASPLLLQIAEELRRRFPRIFGTHRLTQAWAFKHDS
                     ARRGLNIHADAAAVNVNFWITPDEANLNPDSGGLVVWDKEAPRDWDFKTYNSDKARGK
                     IYEWLNAQGAKEIKIPYRANRAVVFNSDLFHETDDIAFKEGFTNRRINITLLYGHRHR
                     L"
     gene            complement(11726..13852)
                     /locus_tag="PK224_16310"
     CDS             complement(11726..13852)
                     /locus_tag="PK224_16310"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRB17359.1"
                     /translation="MTQTRPTILIADDDPLSRLFVRNALEPAGMTVAEATGGQDALVK
                     FEALAPDLVVLDIMMPDMDGYLTCSRLRTLPRGKRVPILILTGLDDAHSIAQAYQHGA
                     TDFITKPVNATILCHHVRYMLRTNNVLHALIRSESRLELAQRIARIGNWDWNPKTNRL
                     SMSNELCRLLGVRPQDFGGTFEAFLNLIHPDDRPVVTGALERLVSQHTPCDIDHRVVL
                     PNGTDFIIHLQAEGVREEETDELTVIGTAQDITERKQAERAIHQLAYYDSLTGLANRV
                     LFKDRLSNALSYAERYHQHLATLFIDLDRFKVINDTLGHTVGDRLLTHVAERLSESVR
                     QSDSVGRHADHEPTHALARLGGDEFTILLTALPTPEDAGRVARRILEALAHPLSIDGH
                     EIFISASIGISIFPSDGATVEALLKNADTAMYHAKEQGRNNCQFYSSGLNAAAAERLD
                     LENELRRALEREEFVVFYQPKLNIHSRKILGAEALVRWKHPKRGLVPPGVFLNAAIDT
                     GLIRPMDEWVLREACRQVKAWETAGLPPITVSANVSNSLFHGRTLPATVADALRDSGL
                     NPSQLELELTESIAMRDVEASVTMLEGLRTMGVRLSIDDFGTGYSSLSYLQRFPLSRL
                     KIDQSFVRDLLTNENNVKITRAIIAMAHSLNLSVLAEGVETEGQLARLREEGCDEVQG
                     YLFSRPVCAEDFEKLLRGDADARTAA"
     gene            complement(14082..14795)
                     /locus_tag="PK224_16315"
     CDS             complement(14082..14795)
                     /locus_tag="PK224_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17360.1"
                     /translation="MAHASRLPRTWLPAVFISVIVSACCAAALYVLLAQHDRSLVSQR
                     NAAAIMAAEQSARAMARTVAWFSESLPGESLGTVQQTLEQHARQANLLDAAVITTDNM
                     IVAASNPAIIGSPMQDAAWPAARNSQSSSITHGVDKGRPVMIVIEPFRQDNRIAGWIR
                     LVVATPPEAAALRSEDDLGRDVALVIGPLLLVMVILLTLTMRGLMSRVRALLAGILME
                     AGEQRFVPAGNPVETPERG"
     gene            complement(14812..15177)
                     /locus_tag="PK224_16320"
     CDS             complement(14812..15177)
                     /locus_tag="PK224_16320"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB17361.1"
                     /translation="MDQRHHPRFPVHFRSSFSSVNRVGGEGNVVDLSLRGCGILSATA
                     VHPGTTLELRIQPAGDAAALTIQQAVVRWCRDGRIGIEFVSLQPDEWTRLQGIVKELT
                     RQPYERANDRQDGPGTSRP"
     gene            complement(15201..16652)
                     /locus_tag="PK224_16325"
     CDS             complement(15201..16652)
                     /locus_tag="PK224_16325"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRB17362.1"
                     /translation="MKLNTDMQVVKINDYLWEIPPSEKPGMLVPARIYASAAILGAMD
                     RGVFDQVTNVACLPGIHRYALCMPDGHWGYGFPIGGVAAFNPEDGVISPGGVGYDINC
                     GMRLIRTDLSLSEVQPKLELLMTELFRRVPAGVGSRGFVNLSRHDFRDVMRQGAGWCI
                     SKGYGWEEDLERIEERGCLEGADPTHVTDYAIERGINQLGTLGSGNHYLEIQVLSDKG
                     IYDRATAAAMGLTGRDQVVVMVHCGSRGFGHQVASDYLKVFEKAMRRYGISVKDQQLA
                     CAPFRSPEGQDYFGAMNCAANTAFANRQVITHQIREAFEAVFGESARRLGMQLIYDVA
                     HNIAKVERYRDGEWLVHRKGATRAFGPGSPELPACYRGIGQPVICGGSMETGSYLLVG
                     TDRAMQETFGSTMHGSGRTMSRAQAKRTVRGEQLMRDMKQHGILVKAVSMSGLAEEAG
                     FAYKNISDVVETVDHAGITKKVAELKPIGNIKG"
     gene            complement(16819..17259)
                     /locus_tag="PK224_16330"
     CDS             complement(16819..17259)
                     /locus_tag="PK224_16330"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HRB17363.1"
                     /translation="MPGSFRFLDDIALADMAFDAEGESLPALFEAATQALIESLADPV
                     TIAETWRHSLDTEEADIETLLFEWLGRLVYLKDAQGVVFHRATLSLNQNGARTAWHLH
                     AELTGAPVDPATQDLRSDVKGVTKHLYAVTQEGTSWKARVVLDV"
     gene            complement(17265..18239)
                     /gene="sdhB"
                     /locus_tag="PK224_16335"
     CDS             complement(17265..18239)
                     /gene="sdhB"
                     /locus_tag="PK224_16335"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HRB17364.1"
                     /translation="MRLTFTIQRFNPETDQQPHREEYRLDIGRGMTVLEALIRIKNEL
                     DGRLALRYSCRSAICGSCAMQINGTEKLACRTSIRKELERHGRISIAPLPNLPVIKDL
                     VVDMSPFWEKIRAVTPWLTPETHPTKRYGSSGQLRLLPESYQFHNVDACIMCGACVAA
                     CTSHEVSRGFLGPAALAKSARFVADPREPAGAKQARLTALQEADGIWDCTRCNMCVQV
                     CPKDVQPMEAIIRLRRSSLRHGLTSAEGARHITGFVDLVRHEGRLNEALMPLKVLGFN
                     LRRVLDVMPLGVRMWLKGKVPLPFGHTIPGIEQVRAIFAAARRRAPRV"
     gene            complement(18319..19476)
                     /locus_tag="PK224_16340"
     CDS             complement(18319..19476)
                     /locus_tag="PK224_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRB17365.1"
                     /translation="MSHSTEHNSRPEQSATLFIAASETDSNLYYATRFIAPDPFIYLE
                     VKGERLMVMSDLEVDRARNQATVDRVLSYSELERRAKSQGVKDPGTIDVIHLVLQDAG
                     LKDILVPPTFPFLHASRLQEMGYRLRTKRDPFYEQRVMKSDDEVRHIEAAQRATETAV
                     AAAHQHLRRAEIRDNVLWLDGEILTSERVKKLINVALMECDCVAQHTIVAGGEQACDP
                     HDEGSGPLPAHRSIIFDVFPRSAGTRYFADMSRTVVRGTPSPELTRLYHAVKDAQEEA
                     ITKIRDGADGAAIHQGICDRFEKAGYKTGLVNGRMQGYFHGTGHGVGLDIHEAPRISR
                     TGSLLQEGHVVTVEPGLYYPGLGAVRIEDMVLVTKDGCRNLTNYPKVFELG"
     gene            complement(19528..>22947)
                     /locus_tag="PK224_16345"
     CDS             complement(19528..>22947)
                     /locus_tag="PK224_16345"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HRB17366.1"
                     /translation="GGVRVRPRVVVLSLLGLVVVGALLLLYSRELFGVDVIKNFFLQQ
                     LETSLRRKIEVDRIKLVVLPSVRLELSNVGVYGHDDPTHVVFQAKEIDIVLRLLPLLK
                     KQVVAKRIFLDEPTVTLIRNRSGHWNVLAGLPSAAKDESAYQMFSRLLQIREATIQQG
                     HITITDEARPDGVRTTKLEAVEMALKVYPGKAQGDLHISASLPAEAAPSSFSLTGTIG
                     LSESSSSLAAEEPYSVHPAFQFDGEIETTNLRLREAADFFGPRPVPAQLQGGANLQSR
                     IRVAPGVAGYDVVLTEIAANVDQLAITGKANMAGLLTSQPTFSITFASPTIDLKTLFA
                     RMPPQWIHPQLPGIVEQRQLGGTVEIVSATLTGATAPSPQLSLTGDFRIEKGTALIGN
                     DRVPTQDLSATISVEPGRIRVGKVTGSYGTLQMTDGKAVVSFLDEGPWMELDISGNMT
                     AADLVKFLTKTIRADRLTSLLAQSREIEGQTHPTFRLVGPLDKPEGITFAGGEVLAEQ
                     VSLVNPSLPQRLTAMHGRILFSQTGGAQFDQVTANVGETQLQFNGMISGGTPSVFQDF
                     VIRAKGSAAQLRQMVSAGTFPDDLLYGMVNAKVQLSGPSGAPHLRGEIGLNDAKLVLP
                     MVGEKPLGSPASLELDADVTRGVGLVISRMELVVPPLRLPLKGHITLGDQFSIDASLA
                     SGTVSLSSLPEWIYRSGFEAGNLEVSMDVKGTDAEWRNWRTGGWLALTNGLMSVKGVD
                     GQVEDIYLRLKFSKNIADIKQLSFRIKDSDVSLSGALKNWTTKPVIAVKIESAQMDLD
                     LLIPKGHRSPIREFLETLASTSQVSATATIEKGLYKHLRFGGLSGRLTIQDGMLDLDR
                     VVAQSGTGHAAGRMVVRLPKGQPAETETSLRMTGIPVESLLPLLGAHDQPVTGEMKLT
                     GVIRGHGRNPHGVLPTLNGKVELVMQDGRILKTEKRAIWKILSILNLPAVLQGKVDLE
                     KEGLQYNRASTTLTIQNGLVKTQNIVLDSPVLKISAVGSYDMPTDQLDMIWAVSPFGS
                     YSQFLKSIPLFGRLIAGDRKGLATALFQVKGSIDDPDVTYMPMKSFTTGLTGVAQLAF
                     DLLKNTVMLPIDILSPQEEKDPMFDPSLEIQTPPSTTPPVETPATPTPATP"
BASE COUNT         4405 a         6674 c         7193 g         4649 t
ORIGIN      
        1 gcccctcgca gatggggctt gaagacctgg cgatgatgtc ggcgcagccc ggtgtgacgg
       61 tgctgtaccc gtccgacgcg ctctcgatgt acaaactggt ggaagcggcg gccgctcata
      121 agggcatggt gtatctccgc gccggtcgcc ccaagacgcc ggtgatttat ggtgtggacg
      181 agcagttccg gatcggaggc tccaaggtgg tgaggcagag tgccgccgat cagctgacga
      241 tcgtggcggc cggcgtgacg ttgttcgagg cgttaaaagc ccatgaccaa ctgaaggcgg
      301 ccggcattac gacccgcgtg gtcgacctct acagcatcgt gccggtggat cgggcgacgt
      361 tgctcgattg tgcgcgggcg accggcggac gattcctgac ggtcgaggac cattatgcac
      421 acggaggcat cggcgatgcc gtgttgagcg cgctcgcctc ggaaggtgtg cgagtgcaca
      481 aattggccgt tcgggaaatc ccgcgcagcg gaaagcccga tgagctcgtc gatcattttg
      541 gaattggagt acgctcgatt gtcgaagcgg ccaaagaaat cgtcaaaaca gcctcccgct
      601 gaacaatccg gccctgcctt ccctgccggg ttaaatcaga ttcccctcct ccagatcctc
      661 agcgcccagg atcgccaaaa ggtcctgggc gaaatgatgg aaacccgtta cggcaagggg
      721 cagtacatct tccgtgaggg cgatcccacc gaatacttcc atatcgtcaa ggaagggtcg
      781 gtgaagtgcg tcaaatcgtc cctcgacggg aaggagtgca cgctgaaagt gctcatgcca
      841 ggagatttat tttgttgcga tgcctcggca ttcgacggcg cgtctcatcc tggaacggct
      901 cagccgatgg gtgatgtcag cgtgctgcgg atgaagaagg aggcctactt taagatgctg
      961 cgtgcgaatc cggatgccgc gatggaagtc atccggtacc tcgggaaccg tctgaatgaa
     1021 gcgcaggaaa aagccaaggt gctcgcgctg gaccgcgcgg accaacgatt ggcctcccta
     1081 ctggtgaatc tggccgaacg aagcggcgtc aaagagccga acgggatccg agtctccgtt
     1141 cggctcacgc gtcaggacat ggcgaacatg gtgggcgtga cgactgaaac cgccatccgc
     1201 atcatggcgc ggtttaagaa agagcgtctc gtctccggaa cggccgctcg tctcatcata
     1261 cgcgacctcc ccaagctcag aatcctcgct tccacgtaat tttttccacg acaaaggcac
     1321 tatcttcata aaacatgaca attgtcatgt tttattgatc atgctctgcc gtatggtgca
     1381 gacagcggcg cagcattcca tgctggaatg cggatctggg agcggatgag cttccaggcg
     1441 cggagggaac cactcgaatc ataggaggca gaaatgcaag aacaggcagg ggataacaat
     1501 caaggacgcg gtcggtgcaa gacggcgtcg ctgctgggat tgacgctggg gatggtggcg
     1561 agtatggcac tgtccgtcca agccaagacc cacgatattc aaatgaccgc agtggagacg
     1621 gacattgtga tcgacggggg cggcgagaag tatgccgcct ggaccttcaa cgggcagttc
     1681 ccaggcccgg tcgttcgggt cacggaaggc gataccatca attttacgtt gacgaactct
     1741 accaccaaca agaatcccca tgccatggac ttccatgcgg cggaaatcga tttcttgaaa
     1801 aactatcgag ccgtcaacac cggcgagagc atcagcttca ccttcgtggc caagaagccc
     1861 ggagtgttct tctaccactg cggggcgcct ccgatgattc agcacgtcgc gcgcggcatg
     1921 ttcggcgcag tcatcgttga tccgaaggat gccaaagtgt ggccgaaggc ggatcgcgaa
     1981 tatgtgctgg tgcaatccga atatttcaag aacccgaacg atgtgcaggc gatgttcgat
     2041 cgcaagtacg acggcatgat gttcaacggc ggcatcttca agtatcaccc ctttgtgacc
     2101 ggcgggggaa aactcgatgc caagccgggt gaacgcgtgc ggatttattt cgtcaatgcg
     2161 ggaccgaacg agttttcttc gttccacccc atcggcgaga tttgggacaa tgtctatgag
     2221 agcggcaacc ccgccaacaa cctgaagggt cttcagacct atgtggtggg gccgggcagc
     2281 gcggccacct tcgacgtggt ggtggagtct gccggagcct atccgctcgt gacccattcg
     2341 ctcaccggcg cgttgcgagg agccattgcg gtgttactgg ccggaccgga tgccaagccg
     2401 gcgccgttga tgccgatggt gccctgggaa ttaccggcga aataacgccg gtcgttggat
     2461 tgattcaccc ggccggctcg aaagggtcgg ccggacagtc cgaaagaaag atcatgacca
     2521 atcaatttgt ccaaggcttg tggtttttgg gagatgccga tgccatttcg tgggatgacg
     2581 cggtgctgct tgctcctgtc cgtcggatgc ctagcgagcc cagtcaatgc cgacacctcg
     2641 cacgagcgcg tggcgctctt cttgtcaggc caagactgtc cctctttccg cccgagtctc
     2701 gctgcggagt tggcgcaggt acccggcgtc acgcgcgtgg agccggacag cgtccctgat
     2761 cacgtgctga tcgatgtggt cagcggccag gtcacttcgg aagatctctt ggcggccgcg
     2821 cgtcgtcgcc tgattcccgg tatgcgttgc cggattgaga tcatgcagtc ctgtatttcc
     2881 gcaggtcccg ctccgttgcc ccggtaagca gcgcttgcga cttttgaacc gttcagacag
     2941 actaggcgcg atctatccga agctctctgc ggagactacc ggcgtcatgg cgcacaaatg
     3001 gaccggcctg ctgtgtggcg cgatcgcaat cgttgggtgg tgtctggcct ctgccgcgtc
     3061 tctgtcggcc caggaggcga tgctggaatt tcccatcagg cccttcaatg tcgatgtcat
     3121 gcttcgccag cagacgttcg cccccgacga ccgcgagatc ggcgtgcaac tcgggatcac
     3181 ggcgctccgc tacggcaacg tggaggttcg gaccatctac caatacttca gcatccattc
     3241 ccaggaattc cggaccgacc agcattccct gtttctcaat ccccgttgga acaacttcat
     3301 cgacatcctg gattttccca cacacaaacc gatcagtcgc ctgctccgtc atatcctgtt
     3361 cggtcccttg gaggatcggg cggtcccgta cgtcgggctc ctggccggag gtgtgctgcc
     3421 cggtccgggg aaccacgcgc cgggccattt gatcggaggt cagctgggcg tgcgatttcc
     3481 tgtggcacgc agtctgtcgg tcgatatcag cgtgcaatat agccagtatg gcgtagattt
     3541 tcgcggcgag gcgggacagg cgcaacaatg ggtatttctc acaggcgttc ggttttgatg
     3601 cgggcgtcgc gagttcgcgt gggatggatg gcggtcctct gtctgtgcct tcccgggtgc
     3661 gggctgttga tcgacgcggt cgagcacgtc tggcctgtgt caggcaacaa ggtcgatatc
     3721 atctgcgaac gtgaacgggt gcaagtcggc ctcgcgatgg agccctttcg tccgtttgtg
     3781 ttcccgacga tctggaccga cgagggcgct cgggtcaccg ggctcgacgt ggaactggtg
     3841 cgggcgatga ccgatgaatt atcccgacgg tgcggacgcg cggtgacccc tgtcttgcac
     3901 ctggtgcggt tcagggatct gttccgactg ctgaatgaag ggcatctcga ttggtttatg
     3961 tccgccgtgg cgatcaacac accagcgccg gcccgggccg gtgtggccta ttccctgccc
     4021 tacttttacg gcggcgggat cagcgggatc acgacaagtc ccgcggttct tgagcgagtc
     4081 cgcgcaaatg tacgagagca ggcgattcat cccactcccg accggctggc ggcgaccagc
     4141 catgcgttgg acggattgac gattgcagta caggacgaaa ccagcgccta ttattacgcc
     4201 gaggcgaatc tgagccccca ccggttgatt ctatgcgatt cgctcccggc cgcgtttgag
     4261 tatgccgact cgcaggcaga gccacgtgtc gacgtgattc tcggtgcgca accggtgctg
     4321 gagtatatgg tcaaacgggt tcgtaaagac tggtcgccgt tggcacggga aaacggccgc
     4381 cccttgtttt tgaccaaagc cgattacgga gttgtggtgg ccgaagagag ttaccacctg
     4441 cggtggttgt tgaacgacct gctgttgaaa ctcgatgagt cgggtcgact tcgcgagatg
     4501 cgaaccaggt ggcttgatga ggagtacgcc tttccacgcc gggcttctct ggaggggttg
     4561 tcgtttgacg tggaacagat ggcggcccat tacgcacaag ggacctgtcg cctcaaggtc
     4621 gagccgtaac atcacggata ggagaatggt catgagaact cacgcgttct gcatcctggc
     4681 ggccgcagtc ctgttagtga tcgcgcaacc gtcgatggtc ggtgcggtcg aacgggcgga
     4741 ggcggaagaa acggcgcggt tgttggcaaa gctcctgcaa tccggccgca tggtgatcga
     4801 acggaatcag acgttgatcg atgatcccca caagggtgag aaagggttca cgccggaact
     4861 attcgagcaa caactcgtgc gggagtttct ggccaagacc gggatcgatc tcaccgcgtt
     4921 gcccgccgcg ccggtttcca tccttatccc gccgttggcg aaagaactct tgcctgcgct
     4981 ggtgcaggcc agccgggaag tggtgcggga tgctcaagtc gtcatcaacc acgcggcatc
     5041 ggctataaga atttcatccc ggcgacgtat gggagtcagg cctcggctcg cttctccaag
     5101 tcggcgcacg tgcgcctgaa acagacggcc atcgagcccc gcaatccgaa aaacgagccg
     5161 gatgaatatg aggcgtcggt gctgaaatgg ctctctgcaa ggccacgcgc ggaagcctat
     5221 gtcagtgagc tgacggaaga ggggcgtatg ttgcgcgtcg tcatgccgat ctattacgcc
     5281 aaggattgcc tcgcctgtca cggcgaaccg aagggggatc tcgacatctc cggctacccc
     5341 aaagaagggc acaaggaagg cgaccttgcc ggggccatca cagtcacggc cccgctaacg
     5401 atccgaaaat gagacggcat gcgtcggtcg ggattctgat aacaggaacg atcgctgatt
     5461 gccggatggc ggttgccgga ggtcaggcct gattcggcgc gacgaacacc aggactttca
     5521 gccgctgttg cgtgtgattc ttcactccat gctcatcgcc ggccggggcc atagtgccct
     5581 ggcccggtcg cagcacctgc ttttctgagc cgacctgaaa cgtcccttcc ccttccagca
     5641 caatgtagac cttatcctgc tcgccgtgaa tgtggccctt ctgttcctgg cccggttcaa
     5701 agcaatagat gtcgcaaaaa aaacgcgggg tctggaagag gttattcttc ttcattttct
     5761 cggaactgaa ctgctgaaaa tctgcgagcg tgagtacctt catgtcgtgg gctccacggg
     5821 tcaggatgaa tgtgttgtgt ccggtgtgtg caggcgcgtc acgatacggt cgacggagat
     5881 caagtggcgt gagagcccca agtcctgtgc agggagaccc atcgcgaggc caagtagctg
     5941 gggaagatgc aggatgggaa ggttcagcgc cgttttgacg gcctgccctg cccgctcctg
     6001 gtaaatgtcg agactcatgt ggcagagcgg acagggagtc accatgaagt ccgcgccatg
     6061 gtccttcgcg tccttcatat tggtgccggc catggccacc gcgatcgctt ccttctccag
     6121 aatgatagga aagccgcagc acttggtccg gcccgcatag gcgaccggtt ccccgccgac
     6181 agcgcgaata attttttcga gcgatgacgg attctcagga tcgtcgaatc ccaactccca
     6241 ggagggccgg agcatgtaac atccgtagaa aggggcgata cgaaaattac gaaatggaga
     6301 gtggaccgtc tcacccacgc gggtcagtcc gatatcccgc accgcgatcc agagtagatg
     6361 tttcacctgg accccgccgc cgtagctgat gccgtcttgc gcgagaattg tattcacccg
     6421 ctcaagggtg cccggttcgt tcttcaggcg gcggtttgcc gcgctcatca ccccctggca
     6481 ggtgccgcag atggtcatga tgtccagccc gagccgctcc gcttgagcga aggtccgggc
     6541 gttcagcgca agggccacat ccggatcggc ctctccgatc acgccggcgc cgcaacagga
     6601 ggaggcggca agctcgacga cctcgatgcc taatcgcccg acgatggcca tcgtggattg
     6661 atagagttcg ggggtggcgc ctttcgctgc gcaaccggga tagagggcaa atttcagggg
     6721 catagagtgg gtcgcctgtt cgtccggtct gtccgtccca acatagcgta tttcacccct
     6781 cgatgaacag acggaatcgg gcttctctcg ggtgcatgag ccataggtgg aatcagggaa
     6841 gttttgaaac ggtctccgta tagcttttct tcaggtcttc gacggcggcg ttcatgttgt
     6901 ctttcagctt actccaggct gagctggtgg aggcattcac ctcctcgagt tgctttcctg
     6961 cccgnnnnnn nnnnnnnnnn nnnnnnnnnn ttccaggtcc tgaagagcct tctgcatttc
     7021 agtccgggct tccgcggagg cggcgtgggt cttcttttgg agttccgcaa ttttgacctg
     7081 catctcggcc agttcggcct gcaccgcctt ctggaaggtc tccttttgct gaagcgtata
     7141 ctgtttggtc gcttcgacgg cctccttggt atccctgatc accctgtcgg ttgtagacgg
     7201 ttgcggctgg tctccggcgg tcgtccgggg aggaagcgtg agggagatgc cgatcatgca
     7261 cagaaacgcc agcgttctag ctacggccat gcggggactc ctttttgttt tcagacggga
     7321 tgcagccatt cggcccaacg cggtcacatt atactcattc tccggcatgt tctgtgcccg
     7381 cagatcccct gccggacggt taacccgcgt tattcatgac ggttcgccgc gaaattgccg
     7441 agtcacgtgg cgagaccggc gcgcggagcc cgggggaccc gaagcgtact cgtgcagtac
     7501 gttgaggatc cgaggggcga gcccgccggc caaaggcccg tcgcagcagt gaatcggcga
     7561 ttgcagcaga agcgctcatg aataatgcgg gttaaggttt tgggaagaat gagccgaaaa
     7621 ggatctacaa ggcagacaat cccgcaacct tgacgtgtgg tgtaagccct atgagcgctg
     7681 aacttcccaa agcgacggac gcggcctcta cggacgcgtt ggaacagcat ctgatcgagc
     7741 gaatcaggaa gggagcgatc gaactccctc tcttgcctca agtagcctcc agaattttga
     7801 gtatggtcta cgatccggat gcggaggcgg ccaagcttgc cgcgctcatt catcaggacc
     7861 aggccctcgc cgcccatgtc atccggattg ccaactctcc ggcttacatg acccgcaatc
     7921 ctgtggtgtc gttacaacat gccgtctcga tgctgggcat gaacttgatg tcggaattgg
     7981 cgttctccgc ctccatcaaa ggcagtgcct tcaaggtgcc ggggtgggat gatgaagtca
     8041 agcgcctctg gcagtattca ctggcaagcg gcgcctacgc caaagaaatt gcccgcgtcc
     8101 gccggttcaa cgttgaaagc gcctatctct gtggactctt gcacgggatc ggcaaaccgg
     8161 tggtgttgca gacgctggtc gcgttggcca aggagcagaa tctcaccttg acgcaggagt
     8221 tgttgcatca actgctggaa ggctactaca tccaggtggg cttgctcgtc gccgaagact
     8281 gggggctccc gccgcctgtc gtggaatcga tcggctttca cagcgattac cagtatgcca
     8341 agacggccaa gcaggagtgc atgacgactt gtctggccgg gcgattggcc aaccattttc
     8401 tcgatcccgt cgcattcgat gaggcgatgg tccgcgagca tgcggtcttt gccgacctga
     8461 acctctatcc caaagatatc gatgcgctgc tggccttgaa agacaaggtg caagccgtcg
     8521 tggaggcgat gccgttgtga tgccccccaa tgcctatgac atcgtggtgg tggggagcgg
     8581 tccggccggc cagaaggccg ccattcaagg cgccaaggcc ggcaagaaag tcgtcctgat
     8641 cgaacaggaa cagggcatcg gcggcaactg tgtgtaccgg ggcacgattc ccagtaagac
     8701 gctgcgcgag acggcattgc agttcgagcg tctcaaacgc tcgagtgaag tgttcgaagg
     8761 acggctgcgg cttgatgtgc ccatgtccgt gctgctgcac cgcctggatg aggtggtgaa
     8821 ggcgcacgag tgttacatgg ccgaccaact cacccgcaac agcgtgacct atcgacacgg
     8881 acgcgcgcga tttctctcgc cgcatgaagt ggaattggag accatcgacg gcgcctgcca
     8941 ggccctgcga gccgacacga tcgtgctggc cacggggtcc cggccgcgtt cgattcctga
     9001 aattcctgtc gatcacgaac atgtgctgga cagcgattcg atcctgtcga tgatctacct
     9061 gccccgctcg ttgacggtct tgggcggagg agtcatcgcc tgcgaatatg cctcgacgtt
     9121 tgcgctgctg ggtgtcgagg tgacgctcat cgacagggcc cagcgccccc tgtcgttcat
     9181 ggatgcggaa atcgtcgagg tgtttcagcg cagcatcgaa cggcaaggcg gacggtttta
     9241 tgtggggcaa accgtcaagg aagtggtgtg ggacggcgta tcctccgtcg tcgccaggct
     9301 ggcgaacggc atggcggtca agagcgaaaa gatgctggtc gcgctggggc gtcaaccgaa
     9361 catcgaagaa ttgaatcttg aggcggccgg gcttaccctg gacgagaagg gccggatccc
     9421 cgtcaacgaa tacgggcaaa cccccgtccc gcatattttt gccgccggcg acatgttggg
     9481 tcgtcccccg gccttggcct cacaagcgat ggaggatggt cgtcgggcgg tcagtcacgc
     9541 cttgggcctt ccagtcggcg actcgctcaa tcaggtgccg atcgggattt ataccattcc
     9601 ggaaatcgcc tccatcggac tcgatgagga acaggctgca gcccggtacc gtggcccgct
     9661 cgtagggcgc gcgcggttta cggaaatcgc caaggggcag atcacgggtt cctgcgacgg
     9721 actcctgaag ctgattgccg acccctccgg tgaacggttg ctcggcgtgc agatcgtcgg
     9781 cgagcatgcg accgaattga ttcaccttgg acagatggcc ttgcaggacg gggcgaccat
     9841 cgaccggttc atcgattcca ttttcagttt ccccacgttt gccgaagggt atcgcgtggc
     9901 cgcgctcgat attctgggcc aacgccgcaa gcgtcagagc accactcaag cggcctaatc
     9961 tcccgctccc ctgccgtctt ttccctcttt gacccccatg ccctatgtgc gctactctcc
    10021 ggggcgaccc gtaaggtcgc cgctggaggg atcttcgtga atcgcaatga gcgccgccgc
    10081 gatgaaaaat tgcatgggaa gcaggccgga ggtgccgatg ccccggcggc gctcctgcgc
    10141 gaggcgcagc tgcatcatca ggccggacgt ctggatgaag cggaacgggg ctaccggtca
    10201 ctactcgagc gcgcaccgac acagccggac gcactccatg gtttgggcct tctgaactac
    10261 cggcgcggtc atttgaacga cgcgctcggg tggctggcaa aggcctgtgc cgccggtccg
    10321 cggaatccga tctattggtt caatcacggc gtcgtgctgc aacgcgcagg ccgcacgccg
    10381 gacgcggtcg aggcctatgg ccaggccctc cactggaatc cccgctacat cgaagcccgc
    10441 accaatctcg ggaacgccta caaagaactg ggacgtcttt cagacgcgca agccgcctat
    10501 gagcaggtcc tgacgctcaa ccccgatctt gccgaggcgc acaacaatct cggcgtcgtg
    10561 ctgaaagaac aggggcggtt ggatgaagcc gcggactcgt atcggcgtgc catcgccttg
    10621 aagccgactc atgcggaggc acagaacaat ctcggcctgg tgctcctgga acagggtcgg
    10681 ttggacgatg ccattcgctg ctttgaacgc gcgcttcaga tcgtttccgg ttatggaacc
    10741 gcgctctaca acctcggtat tgcctggatc tggcgggagg atataccgcg ggcgctgcgc
    10801 tgttttgccg aaaccgcgca agccaaacat gcgcacggcc gccctgtggc agagacgacc
    10861 gtctttcgct cccggctcaa gcatgatgcc gagcagctag agtatcttcg agagagcggg
    10921 ctgttgggcg atgagtggaa cccctatcac gaggccctca ctcgactgtg cgaaaaattg
    10981 gaacactcgg cgaccggtgc gaccggatcc tcaaatcgcg tgctgctgtc gcctgccgac
    11041 atgcagccca ttgcggcctc gtataaccgg ctgatccata tcgctccttg cgaggcgatc
    11101 gagggagggg cgctggctgc ggatctggac agcgcagccg tggaggcccg ctatctggcg
    11161 acgaatccgg aggtgacgta catcgacggg ttgttggcgg acgaagcgtt gcaacggttg
    11221 agacagttct gctgggcgtc cacgatctgg aagaaggact acgagaacgg ctatatcggg
    11281 gccttcctcg gtgatggatt cgcctctccc ctgctgcttc aaattgccga ggagttacgc
    11341 cgccggtttc cccgcatttt cgggacgcat cgcctgacgc aggcctgggc attcaagcac
    11401 gacagcgcca ggcgcggctt gaacattcac gccgatgccg cggcggtcaa cgtcaatttc
    11461 tggatcacgc cggatgaggc gaacctgaat cctgacagtg gtggattggt ggtgtgggat
    11521 aaggaagcgc ccagggattg ggatttcaag acgtacaata gcgacaaggc ccgaggtaag
    11581 atttacgagt ggctgaacgc gcagggggcg aaggaaatca agattccgta tcgggcgaac
    11641 cgggcggttg tgttcaactc agatctgttc cacgaaaccg atgacattgc cttcaaggaa
    11701 gggttcacca accggcgtat caatatcacg ctgctgtacg ggcatcggca tcgcctctga
    11761 gtaacttctc aaaatcttcc gcgcagacag gccggctgaa caggtagcct tgcacctcgt
    11821 cgcatccttc ttcccgtaac cgtgccaact gaccttctgt ttccactcct tcggccaaga
    11881 ccgagagatt cagactgtgc gccatcgcga tgatcgcccg ggtgatcttc acgttgtttt
    11941 cgttggtgag cagatcgcgg acaaacgatt gatcgatctt caaacggctc aggggaaaac
    12001 gctgaagata actgagggac gaatacccgg tgccgaagtc gtcgatggag aggcggacac
    12061 ccatggtgcg gaggccttcc agcatggtga ccgacgcctc gacgtcccgc atggcgatgg
    12121 actcggtcag ttccagctcc agttgcgatg gattcagccc ggagtcccga agcgcatcgg
    12181 ccaccgtggc gggcagcgtc cgtccatgaa acagggaatt ggacacgttg gccgacacgg
    12241 tgatgggcgg caggccggcc gtttcccagg ccttcacctg gcggcaggct tcgcggagca
    12301 cccattcgtc catcgggcgg atgaggcccg tgtcgatggc cgcattcaag aagactcccg
    12361 gcggcaccag gccgcgtttg ggatgtttcc aacggacgag cgcttccgcg cccagtatct
    12421 tgcgtgaatg gatgttcagc ttcggctgat agaagacgac aaactcttcc cgttccaagg
    12481 cgcggcggag ttcgttctcc aggtcgaggc gttcggccgc ggcggcgttc aagccggacg
    12541 aataaaactg gcagttgttc cgcccctgct ctttcgcgtg gtacatcgcg gtgtcggcgt
    12601 tcttgagcag cgcttccacg gtggcgccgt ccgagggaaa gatcgaaatg ccgatgctcg
    12661 cggaaataaa aatttcatgc ccgtcgatgc tgagggggtg cgccaatgcc tccagtatcc
    12721 gacgggccac ccggcctgcg tcttccggcg tcggcaaggc cgtgagcaaa atggtgaact
    12781 catcgccgcc gagtcgcgcg agggcatgcg tcggttcgtg gtcggcatgc cggccgaccg
    12841 agtcgctctg acgaaccgac tcactcaacc gttctgccac atgtgtcagc aagcggtctc
    12901 ccaccgtgtg ccccagtgta tcgttgatca ccttgaaccg atccaagtcg ataaacaggg
    12961 tggccagatg ctgatggtag cgttcggcat aggacagggc gttggagagg cgatctttga
    13021 acaacacacg gttggccagg cccgtcaggc tgtcgtagta cgcgagttga tggatggccc
    13081 gttcagcctg tttgcgttcg gtgatgtcct gggcggtccc gatgactgtc agctcgtccg
    13141 tctcttcttc gcgcacccct tcggcctgga gatgaatgat gaagtcggtc ccgttcggca
    13201 acacgacgcg gtggtcgata tcgcatggcg tgtgctgcga gaccaatcgc tccagcgcgc
    13261 cggtcacgac cggtcggtcg tcggggtgaa tcagattcag gaaggcctcg aaggtgccgc
    13321 cgaaatcctg cggacgcacg cccagcaggc ggcataattc attggacatg ctcaaacgat
    13381 tggtcttggg attccagtcc cagttgccga tccgggcgat ccgctgggcc aattccagcc
    13441 gcgattcgct tcggatgagc gcgtgcagca cgttgttggt tctgagcatg taccggacat
    13501 ggtggcagag aatcgtggcg ttgaccggtt tggtgatgaa atccgtagcg ccgtgctgat
    13561 aggcctgcgc gatggaatgt gcgtcatcca atcccgtgag gatcaaaatc gggacgcgtt
    13621 tgccgcgagg aagcgtgcga agccgggaac aggtcagata cccgtccatg tccggcatca
    13681 taatatccag gacgaccaga tcaggcgcca gtgcctcgaa tttcaccagc gcgtcctgcc
    13741 ctccggtggc ctcggccacg gtcatgccgg ccggttccaa tgcgttccga acaaagagcc
    13801 gtgagagcgg gtcgtcgtcg gcgatcagga tcgtgggacg ggtttgagtc atcgtatggt
    13861 cttcaggcac aggtggttat agcgcgggcg catgggttcc cggggcgaga atgcctcggg
    13921 tgcctctgac aggcctgcct gtttcgctct cgtgtgagag tatcaaactt ctgaataagc
    13981 gctacataat tagcacttgt tgagcagcgt aggccgctcc catccctaag tggaaatctg
    14041 gtgaacgtaa ggccgacggc gggaatgtcc gtgccggtga atcaacccct ctccggtgtc
    14101 tcgacgggat tcccggccgg gacgaagcgc tgttccccgg cttccatgag gatgccggcc
    14161 aagagtgcgc gaacccggct catgagtcct cgcatggtca gggtcaagag gatgaccatc
    14221 acgaggagca gcggtccgat gaccagggcc acgtcccgcc ccagatcatc ctccgaacga
    14281 agggctgcgg cctccggcgg ggttgcgaca accagtctga tccatcctgc gatacggttg
    14341 tcctggcgga agggctcgat cacgatcatg accggcctcc ccttgtctac cccgtgagtg
    14401 atggaactgc tctgactgtt gcgtgcagcc ggccatgcgg catcctgcat cgggctgccg
    14461 atgatggccg gattactggc ggctacgatc atgttgtccg ttgtgatgac ggcggcatcg
    14521 agcagattgg cctgtctggc atgttgttcc agggtctgtt gaacggtgcc cagactctcg
    14581 ccggggagcg actcactgaa ccaggccacg gttcgcgcca tggcgcgtgc gctctgctcg
    14641 gccgccatga tggccgccgc attgcgctgt gagacgagcg aacgatcgtg ctgagccagg
    14701 agcacataga gcgccgccgc gcagcaggcg gagacgatca cgctgatgaa caccgccgga
    14761 agccaggtgc gaggcaatcg ggacgcatga gccatgctgt atcctcctcg gtcagggtcg
    14821 tgacgtgccg ggaccgtctt gccggtcgtt ggcccgttca tagggttggc gcgtgagttc
    14881 tttgacaatg ccttggaggc gagtccattc gtccggttgc agggacacga attcgatgcc
    14941 gatgcggccg tcgcggcacc agcgtaccac ggcttgctga atggtcagag cggcggcgtc
    15001 acccgcgggc tggatgcgga gttcaagggt ggtgccggga tgcacggctg tcgcgctcag
    15061 gatgccgcaa ccgcgcaggg acagatcgac gacgttgccc tccccgccga cgcggttgac
    15121 cgaactgaag gaacttcgaa agtgaactgg aaaacgagga tggtggcgtt ggtccatgat
    15181 cggctagcgc tggtataagg ctagcccttt atattgccga ttggtttgag ttccgctact
    15241 ttttttgtga ttcccgcgtg gtccaccgtc tcgacgacat cggaaatgtt cttgtacgcg
    15301 aacccggctt cttcagccag acccgacatg gatacggctt tgaccagaat gccgtgctgt
    15361 ttcatgtcgc gcatgagctg ctcgccgcgc accgtgcgtt tcgcctgcgc gcgcgacatg
    15421 gtccggccgg acccgtgcat ggtcgagccg aacgtctcct gcatcgcccg atccgtgccg
    15481 acgagaagat acgagccggt ctccatggag ccgccgcaga tgaccggctg gccgatccct
    15541 cgataacagg ccgggagttc agggctcccg ggtccgaatg cccgcgtggc gcctttccgg
    15601 tgcacgagcc actccccgtc ccggtagcgt tccaccttgg cgatgttatg cgccacatcg
    15661 tagatcagct gcatgcccag gcgccgggcg ctttctccga acaccgcttc gaaggcctcg
    15721 cgaatctgat gcgtgatgac ttgccggttg gcgaacgcgg tgttggccgc gcagttcatc
    15781 gcgccgaaat agtcctgtcc ttccggtgag cgaaacggcg cacaggcgag ttgctgatct
    15841 ttgacggaga tcccgtaccg gcgcatggct ttctcgaaca ccttgaggta gtcactcgcc
    15901 acttggtggc cgaatccgcg cgaaccgcaa tggaccatca cgacaacctg atcgcgaccg
    15961 gtcaatccca tggccgcggc ggtcgcacga tcgtagatcc ctttgtcgga gagcacctgt
    16021 atttccaggt agtggttgcc ggatcccagg gtgccgagtt ggttgatgcc gcgttcgatg
    16081 gcgtagtccg tcacgtgggt ggggtctgcg ccctccagac aaccgcgttc ctcgatgcgt
    16141 tcgaggtcct cctcccagcc atatcctttg gagatgcacc agccggctcc ctgccgcata
    16201 acgtcgcgaa agtcatgccg ggaaagattc acgaagccgc gcgatccgac accggccggg
    16261 acgcgccgga agagttccgt catcagcagt tccagtttcg gttgcacctc ggagagcgac
    16321 aagtcggtac ggatcagccg catcccgcag ttgatatcgt agccgactcc gccgggtgag
    16381 atgactccat cctcaggatt gaatgcggcc acaccgccga tgggaaagcc gtacccccaa
    16441 tgtccatcgg gcatacagag ggcataacga tggataccgg ggagacaggc cacgttggtg
    16501 acctgatcga agacgccccg atccatcgca ccgagaatcg ccgcgctggc gtagatgcga
    16561 gccgggacca gcatgcccgg tttttcagag ggcggaattt cccagaggta gtcgttgatc
    16621 ttgacgacct gcatatcggt gttcagtttc atacgtccag ccggatgctg aaaaaggcct
    16681 ccaacgtcgt gctcgggcac tcaatcccct cgacgtacgc tgagtacgcc tcggggcctt
    16741 cgcgtcctgc ggcctagttg gcgatccttt ttgagcatcc ttgtattgaa tgaaggtgat
    16801 tctgcgcgaa ggggtgtttc atacgtccag caccaccctg gccttccagc tcgtcccttc
    16861 ttgtgtgacc gcatacagat gtttggtgac gcctttgacg tctgagcgca ggtcctgcgt
    16921 cgcgggatcg accggtgcac cggtcagttc ggcatggagg tgccaggctg tgcgggcccc
    16981 attctgattc agcgacaatg tcgcccgatg gaagaccacg ccctgcgcat ctttcaaata
    17041 gacgagccgt cccagccatt cgaacaggag cgtctcgatg tccgcttctt ctgtgtccag
    17101 gctgtgccgc cacgtttcgg cgatggtgac aggatcggcc aggctttcga tgagagcttg
    17161 cgtcgcggcc tcgaagaggg ccggcaaaga ctctccctcg gcatcgaatg ccatatcggc
    17221 cagcgcgatg tcgtcaagga atcggaaaga gcccggcatg ggcgtcagac gcgcggagcc
    17281 cgacggcgcg cagcggcaaa gatggcccgg acctgttcga tgcccggaat ggtgtggccg
    17341 aacggcagcg gtacctttcc tttgagccac atacgcacgc cgagcggcat gacatcgagc
    17401 acgcgtcgaa gattgaaccc aaggactttc agcggcatca acgcttcatt caaacggccc
    17461 tcgtggcgga ccaggtcgac gaagccggtg atgtgacgag cgccttcggc tgaggtcaat
    17521 ccatgccgga gggacgagcg acgcaaacga atgatcgcct ccatcggctg cacatccttc
    17581 gggcagactt gcacgcacat gttgcagcgg gtgcagtccc agatgccatc ggcctcctgg
    17641 agcgcggtga gtctggcctg tttcgccccg gccggttcgc gcgggtctgc gacgaatcgc
    17701 gcggacttcg cgagcgccgc cgggccgaga aaaccccgtg agacttcatg cgacgtgcag
    17761 gcggccacac aggctccgca catgatacag gcgtctacgt tatgaaactg gtacgactcc
    17821 ggcagcagcc gaagttgtcc ggatgacccg tatcgtttgg tgggatgggt ctcgggcgtc
    17881 agccagggcg tgacggcccg gatcttctcc cagaacggcg acatatcgac caccaggtct
    17941 ttgatgaccg ggaggttggg caagggcgcg atcgagattc tgccgtggcg ttcgagttct
    18001 ttgcgaatgg aggtccggca ggcgagcttt tccgtgccgt tgatctgcat ggcgcaggag
    18061 ccgcaaatgg ccgagcgaca ggaataacgc agcgccaacc ggccgtcgag ttcgttctta
    18121 atgcggatca gggcctcgag cacggtcatg ccgcgaccga tgtcgagacg gtactcttcc
    18181 cggtgaggct gttgatcggt ttcgggatta aaacgctgga tggtaaaggt caggcgcata
    18241 acatcgtgag gcgtgagacg taaaacgtga aacgggaaaa gagagccgcc cctatattga
    18301 aggaccgctt accatgggtc agccgagttc gaagactttc gggtaattcg tcaaattccg
    18361 gcagccgtcc ttcgtcacga ggaccatgtc ttcgatacgt acggcgccaa ggcccgggta
    18421 gtagagtccc ggctcgacgg tcacgacatg tccttcctgc agcagcgaac cggtccggct
    18481 gatacggggc gcttcgtgaa tatccaagcc gacgccatgg ccggtgccgt gaaaataccc
    18541 ctgcatgcgg ccgttgacca ggccggtctt gtagcccgcc ttctcgaacc gatcgcagat
    18601 gccctgatgg atggcagccc cgtcggcgcc gtcgcgaatc ttggtgatgg cttcttcctg
    18661 cgcgtctttg acggcgtgat agaggcgcgt caattccggc gagggtgtgc cgcggacgac
    18721 cgtgcgggac atgtcggcga agtaacgggt tccggccgag cgcggaaaaa catcgaaaat
    18781 aatgctgcga tgagccggca gcgggccgct gccctcatca tggggatcgc aggcctgttc
    18841 cccgcctgcc acgatggtat gttgcgcgac gcagtcacat tccatcaggg cgacgttgat
    18901 caattttttg acccgctcgg aggtgagaat ctccccgtcc agccagagca cgttgtcgcg
    18961 aatctcggcg cgacggaggt gttggtgcgc ggcggctacg gctgtttccg tcgctcgctg
    19021 ggcggcttcg atgtgccgaa cctcgtcgtc cgatttcatc acccgctgtt cgtaaaaggg
    19081 atcgcgtttg gtgcgcagcc gatagcccat ctcctggagt cggctcgcat ggagaaaggg
    19141 aaacgtcgga ggcaccagaa tgtctttcag cccggcatcc tgcaagacta gatgaatcac
    19201 atcgatcgtg cctggatctt tgacgccctg cgatttggcg cgccgttcca actcggaata
    19261 ggacaacacg cgatcgaccg tcgcctggtt acgcgcccgg tccacctcca ggtcgctcat
    19321 gaccatgagg cgttcgccct tcacttcaag gtagatgaag ggatccggcg cgatgaagcg
    19381 ggtggcgtaa tagagattgg aatcggtttc gctggcggcg atgaagaggg tcgcgctctg
    19441 ctcgggacgg ctgttgtgtt cggtcgagtg gctcattaga tggatgcgtt cacgtcattc
    19501 ggatgctatc acgcggtcct gaggtggtca aggcgtggcc ggtgtcggag tcgcgggtgt
    19561 ctcgaccggc ggcgtggtag aaggcggcgt ctgaatttcc agcgagggat cgaacatcgg
    19621 gtccttctcc tcctgcggcg acagaatatc gatcgggagc atcaccgtat tcttcagcag
    19681 gtcgaacgcg agctgcgcga caccggtcaa tcccgtggta aaggatttca tcggcatgta
    19741 agtcacgtcc ggatcgtcga tggagccctt cacttgaaat agcgccgtcg ccaagccctt
    19801 gcgatccccg gcgatcagcc gcccgaagag cggaatcgat ttcaggaatt gtgaatagga
    19861 tccgaaggga ctcaccgccc aaatcatgtc gagctgatcg gtaggcatgt cataactgcc
    19921 gacggcggaa atcttcagca ccggactgtc caggacgata ttttgcgtct tcaccaatcc
    19981 gttttgaatc gtgagggtcg tgctcgcccg gttgtactgc aatccctctt tctccagatc
    20041 caccttgcct tgcaagaccg cgggcaggtt cagaatggag agaattttcc agatggcgcg
    20101 tttctcggtc ttgaggatgc ggccatcctg cattaccagt tcgaccttgc cattgagggt
    20161 cggcaggact ccgtgggggt tccgtccgtg cccgcggatg acaccggtca gtttcatctc
    20221 gccggtcacc ggctgatcat gggctccgag caacggcaac agggattcga ccgggatgcc
    20281 cgtcatcctg agcgaagtct cggtttcggc cggttgtccc ttgggcagtc ggacgaccat
    20341 gcgtccggcg gcatggccgg ttcccgattg agctacgaca cggtcgagat ccagcatgcc
    20401 gtcttgaatg gtcaatcgcc cggataaacc gccgaaccgc agatgtttgt agaggccttt
    20461 ttcaatcgtg gcggtggcgc tgacctggct ggtcgaggcc agggtctcaa gaaattcacg
    20521 gatcggtgag cggtgcccct tgggaatgag caggtcgaga tccatctggg ctgattcgat
    20581 cttcaccgca atgaccggtt tcgtcgtcca attcttgagc gcccccgaca gactgacatc
    20641 gctgtctttg atgcggaacg agagctgttt gatgtcggcg atgttcttgg agaatttcag
    20701 ccggaggtaa atatcctcga cctggccgtc aacacccttc accgacatca gcccgttggt
    20761 gagcgccagc catccgcccg tgcgccagtt tctccattcg gcatccgtgc ccttcacatc
    20821 catggaaact tcgagatttc ccgcctcgaa cccgctgcgg tagatccatt cgggaaggct
    20881 cgacaatgag accgttccgg aagcgagtga ggcgtcgatc gaaaattgat cgcccagggt
    20941 gatgtgtcct tttaacggca accgcaacgg cggtaccacg agttccatgc gggaaatcac
    21001 gaggccgact ccccgcgtga catcggcatc gagctccagg gaggccggtg aaccgagcgg
    21061 cttctccccg accatcggca gcacgagttt ggcgtcgttc aggccgatct ctccgcgcag
    21121 atgcggtgca ccagacggac cggataactg gaccttcgcg ttgaccatgc catagaggag
    21181 atcgtcgggg aaggttccgg cggagaccat ctggcggagc tgggcggcgc tgcccttggc
    21241 gcggatgaca aaatcctgaa agacgctggg cgtgccgccg ctgatcatgc cgttgaactg
    21301 cagttgcgtc tcgcccacgt tggcggtgac ttgatcgaat tgggccccgc cggtttgcga
    21361 gaacaggatg cgcccgtgca tggccgtgag ccgttgcggc agggagggat tcaccaggct
    21421 gacctgctcg gccagcactt ccccgcctgc gaacgtgatg ccttccggtt tgtccagcgg
    21481 accgacgaga cggaaggtcg gatgcgtttg gccttcgatc tcacgcgatt gcgcgagcag
    21541 cgaggtcagc cggtccgcgc ggatggtttt ggtcaaaaac ttcaccagat ccgccgccgt
    21601 catgttgccg ctgatgtcca gctccatcca gggcccttca tccaggaacg acaccacggc
    21661 ttttccgtcg gtcatttgga gcgtgccgta gctccccgtc accttgccga cccggatacg
    21721 tcccggctcc acggagatgg tggcggacag atcctgcgtc ggcacacggt cgtttccgat
    21781 cagcgccgtg cccttctcaa tccggaagtc gcctgtgagc gacagttgcg ggctgggcgc
    21841 ggtggcgccg gtcagcgtgg ccgagacgat ttcgaccgtc ccgcccagtt ggcgctgttc
    21901 gacgatgccg ggcaattggg gatgaatcca ttgcggtggc atgcgggcga acagcgtctt
    21961 gagatcgatg gtgggcgacg caaaggtgat ggagaacgtg ggttgcgagg tcaggagtcc
    22021 cgccatgttc gccttgccgg tgatcgccag ttgatcgaca ttggcggcga tctccgtgag
    22081 caccacgtca tacccggcca cgccgggcgc cacgcggatg cggctctgca gattggcgcc
    22141 accctgcagt tgagcgggca cggggcgcgg gccgaagaag tcggccgctt cgcgcaggcg
    22201 caggttggtg gtttcgatct cgccgtcgaa ctgaaacgcg ggatggacgg aatagggttc
    22261 ttccgccgcc agcgacgagg aggactcgct caagccgatg gtgccggtta aggaaaatga
    22321 agaaggggcc gcttctgcgg gcaaagaggc ggagatgtgc aagtcgccct gggccttgcc
    22381 aggatagacc ttcaatgcca tttcgaccgc ttccagcttg gtggtacgca cgccgtccgg
    22441 acgggcctcg tccgtgatcg tgatgtgccc ctgctgaatc gtggcctcac ggatttgcag
    22501 gagacggctg aacatctgat aggccgattc gtctttggcc gcggagggga gcccggcgag
    22561 cacattccaa tggccggagc gattgcggat cagcgtgacg gtcggctcat cgagaaagat
    22621 tcgtttggcc acgacctgct tcttcagcag cggcaagagg cgcaggacga tgtcgatttc
    22681 tttggcctgg aagacgacgt gggtcgggtc gtcgtgacca tagacaccca cattggagag
    22741 ttcgagccgg acgctgggca ggacgaccag cttgatccgg tcgacctcga tcttgcgccg
    22801 cagactggtt tcgagttgct ggaggaagaa attcttgatg acgtcgacgc cgaacaactc
    22861 gcgcgaatag aggagaagga gcgcacccac gaccaccagg ccgagcagcg ataacacgac
    22921 aacgcgcgga cgaaccctca ctccacc
//
LOCUS       DAORRQ010000063        22784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_137_length_22784_cov_56.966343, whole genome shotgun sequence.
ACCESSION   DAORRQ010000063 DAORRQ010000000
VERSION     DAORRQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22784
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_22784_cov_56.966343"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..564
                     /locus_tag="PK224_16350"
     CDS             106..564
                     /locus_tag="PK224_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17367.1"
                     /translation="MKTQVDQRVGRMGLYGLVGLAIMLWAPGVSAADGKAEKSFTIAV
                     TDQQGVETELKNGIFYWEEKMSETSFVPHELRHLPAKRGTATVNIKFDQIKQIEIKPG
                     ADKTAPAMTVTLTNGKNGEFTPAVSGSFKGESDFGQVEVPVGSVSKVIFK"
     gene            599..1732
                     /locus_tag="PK224_16355"
     CDS             599..1732
                     /locus_tag="PK224_16355"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRB17368.1"
                     /translation="MENVLVAQSWQPKRSMTGWGASLLLHACLALAAFGLMPKMAMVV
                     EKEPFKWDVALVEAPREVVRQEEPAPVPQVQPPVPTPVKPRPEPVRAVPPPPQPVQRQ
                     VETRVVPQAIQREVQPVVETVRPQEQIQPAREVVQVQAKPIEPQEIQKTEPLMQAAVP
                     AEVVRETAPAVEQTVVEAAPATAQTYQAQAVVSEPTVMETRPEPVVTASAPVVHAAPA
                     AEPAPASPAPAVEAVASVSEPVPAPPAPVAAVPAPVAAAQPDPATLQEHQVVARVATP
                     RPATKADYGWLAESLHRRIIELRHYPSTARLNGWEGKVVLKVSIRQDGQLKDVEVVKS
                     SGHESLDQAAMEAVRRACPLHMKHELTAPMVVLHLPVSYSLNR"
     gene            1799..2167
                     /locus_tag="PK224_16360"
     CDS             1799..2167
                     /locus_tag="PK224_16360"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HRB17369.1"
                     /translation="MDRVSKDLRPVGFPILVCAGILAAWWIAAGCVWAGDGQAVGSDS
                     ARAQRIEELQKHRERIERELNQLKQQPDGVSRSSVPRTELSNQPTRTLKESMESVPGV
                     ASSPGAEGRSVDLSIRGAGK"
     gene            2190..3467
                     /locus_tag="PK224_16365"
     CDS             2190..3467
                     /locus_tag="PK224_16365"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRB17370.1"
                     /translation="MGQSETSLGGLLALCLAAAIPIAAAQAESAVPFELSQKFVTEHK
                     VKSLVGPSVQVDEQGQVSLAWMEEEKDVRSVLYARSAEAGGPMGVPVRINRPEDVPYW
                     RQEAPALVVQGEDVFVTWGLAHPKATPQQPFATELRLSRSIDGGKTFQPSVAVNDDPG
                     VIQHTFDALHRDADGRLHLSWIDGREGKKEPGTYVARSLDRGATITRNLKVDEGTCVC
                     CRTAVTSGPEGMVYVAWRKIFEGNVRETVVARSTDHGETFEAPVIVGHDRWVFPACPH
                     RPASMGVDRQGRLYVVWYTEGTDEIPAVYIAYSDDRGKSFSEKRKLNVSKNTFPDHPQ
                     IAVDPEGRIVVVWEEQAPVKRDVVMSVSMDRGATFTAPHKVNEKKSQTPVVAVNSQGL
                     FALAWMEHGMPGHKIVMQTVKVPSVKVAAEPVR"
     gene            3492..4025
                     /locus_tag="PK224_16370"
     CDS             3492..4025
                     /locus_tag="PK224_16370"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRB17371.1"
                     /translation="MMPTGLLRGLMLAVVLSTTAGPVWADDPFAALKIARVAPGTAAA
                     AFELQSLDGRSVQLADLKGKVVVVNFWATWCGPCKEEMPAFERLRRQLDPDRFVLLTI
                     TTDLQRAGIKQFLANLNVQLPVLFDEQQDVSQAYLVRALPTTVFIDRQGALLGRAVGP
                     REWDAPKAVQALQGLMP"
     gene            4022..4342
                     /locus_tag="PK224_16375"
     CDS             4022..4342
                     /locus_tag="PK224_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17372.1"
                     /translation="MSRLKTPSVGLAAALAGIYLVLAAFSMACAFDHADPMSAGHHHG
                     GTVSHSSFCNWACQANPTSDAAASALVLHPFLVVALFVERDHTVIAGGAGFHAASRAP
                     PVQS"
     gene            4452..6776
                     /locus_tag="PK224_16380"
     CDS             4452..6776
                     /locus_tag="PK224_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB17373.1"
                     /translation="MWARRNSSQIQFILLFTLVVALPFTASRALAQEEAAAGAVVEKS
                     ARERDLRDQLQKILHELEELRQAPAGGATAAPVQPSVVQEPSEGKAAEAIPQFELADV
                     SIVSDRVQRRPEGITLSATEQSETESQPTRTLRESMESLPGVILRQANGPRDFSISIR
                     GSGVKTSFAVREIKMYEDGFIQTQSDGLSRLDIHDPWFMRSVEVTRGASSSLYDNYAL
                     GGMVHFRTRRGSDINGMESFISGGSYGYQKYGFAVGQQFDNVDISLFGSHVAEDGFIR
                     HSDYSTQTMNFNFRVKVDDKQNLYFKAITNWLDSRVPTRLTLSQFDADHRQAGGINNA
                     TNAVLLGQRRLDRRNIIGGMYERQIDANTVLTMEADYDVKDINQTFTQISDNVNPNYK
                     HYTDLRHDGRLFDMPLRSYVGFFVNQMEQEGNTFNNLQDFFGTRGTLAQNSRGTIRNI
                     GGRFREELEFIPKWTLAAGLGFEQSQLSVQTINYTAGAVSGRSGAERSFSNWAPELSV
                     SWKPADGYRHWVRASTGYAIPGFGNLLTNPFTGLAGTNFDLKPQKNLNVEIGTESRLH
                     KTLTVQLVGFVTFVKDEIITQVVSNTGGQAAINADSSQYRGIELFYDWRPLDGLRLSG
                     AYTHIDARYINFSDRFLAGGIPTAFVRDGKAVPNVPTDTINFKESYDHAASGWGGWVE
                     VNYMNSYFLNNNNTVGIPAYVIANLNIHKNFEFHNNKWIRFAKFFAEIDNIADKKYAA
                     SGNVVADSTADANKTLFFAGYGRSFYAGVTLGLF"
     gene            6838..7032
                     /locus_tag="PK224_16385"
     CDS             6838..7032
                     /locus_tag="PK224_16385"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB17374.1"
                     /translation="MNKQLLRVGEAADVLAVSRWTIYRWVEEGRLEGTKIGRGSLRVF
                     RTSLDRLVQNNKTQEMNLTV"
     gene            7106..8515
                     /locus_tag="PK224_16390"
     CDS             7106..8515
                     /locus_tag="PK224_16390"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRB17375.1"
                     /translation="MHRILLCLVFLLAGCAGGTGLSSSSKASQQTGPSGSDSYAFING
                     RWFDGKGFQAATWYSVQGRLTRTPPQGKVETVDLSGLFVVPPFGEAHNHNVEGPWNVQ
                     AVVERYLKDGVFYVKNPNNVRDFALQIRSAVNQPASIDATFAHAGLTGQGGHPIALYE
                     DVLRLGRYEPAIGTVERGWFENRSYIVLDTDADVETKWPVITSGRPDFLKVYLVHSED
                     DDATGKSGHSPQRSGLHPRLVAQIVAKAHAAGLQVTAHVETAADFRHAIRAGVDEIAH
                     VPGWLVNSAADAESARLTEADARLALERHVRVVTTAVAGSAMPSVTGHHPHGHYEGHA
                     TGPSDHHATAPDESLSQVLKDNIALLHRVGVRVVVGSDHAETSLAEVMQLHRFHLFDN
                     LTLLKMWCEDTPAAIFPDRRIGRFEEGYEASFVALAGNPIEDFSQVQRIVRRFKQGVN
                     LDRSFRHDAAPSGAGHRAH"
     gene            8591..10852
                     /locus_tag="PK224_16395"
     CDS             8591..10852
                     /locus_tag="PK224_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB17376.1"
                     /translation="MKAVVWWGRGTAAACLVAALMVGIEPSVAQAAGEPSDQEAPVVV
                     VDPVEVKGKRIENVEDVKQELARRPGSNILIEEKQITESRALNLQDVLQFAPGVRFQS
                     RFGADEGQFQIRGTSLRNNFHHRGINILINGIFFGDADGFSDFESIDLLAYERIEVYK
                     GANALRYGANSIGGAINFVPRTGYSASTLQMRMLGGSFGMVSGQVSSGKVLQPFKVGN
                     MSATMDYYISVSGNRQDGYQDNSQQARERVNANIGLQLGNHQEIRAYFLQANVAERIP
                     GSLTTGQLFQNRQQAGGQSPPGTPPFFACNQNNQVCNYGRYYNLQRIGIAYHNEFAPN
                     QYFEIIPYFSNQYVDHPIFQTIRQENNNVGGEFRYVNSNSLFGKNNSFVAGFQPRYGN
                     QRQQRFVNINGSIGALTQNYTAKTTYFGMYAEDAFDATKDFTIVLGGRWDYTGRQATV
                     DNFGPAGNPFNPNTPSTPTGTNRPLQHFDAISPKIGFVYRTTPTSQLYFNASRAYEAP
                     LNLELLSSVNANGTPNTGFLNLDAQRAWQLELGHRGTSADKRYTWDVTVYNLEMQKEI
                     LASIINNQGTFQNANGTRHTGVEAGGGMVLGKGLFAQGGAGREDSLQTRVAYTWSRFK
                     FTDDVRGGGAVGPNVLIAKDGNTVAGAPEHSLNVEFRYDHPSGWWIAPNIEWSLSGFY
                     TDYQNTIKNPSYALINLRSGWNVDEHWTLFAEGRNLTDKTYAGAVVVNDQFNRYANAG
                     LGISAFGGVEYKF"
     gene            11094..13058
                     /locus_tag="PK224_16400"
     CDS             11094..13058
                     /locus_tag="PK224_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRB17377.1"
                     /translation="MYGLFIFFVLVWSVVPVYADSSSGPPSVEAFASLPRIASIQLSP
                     SGRHLAVLRHHEGKTYLDTQTVTGQDAHRVVTTDNQEYVITWFRWANDERVLVSVRFA
                     DSREGVDSQETRLLAVNRDGTQQNGNLLRVSSFPSIFGKKHFPQFQDRLVGTIPGDPR
                     HVLIALDIERPLSPDVYTMDVYSGERTLVQNNPGLQPGPRNVLQWIADREGRLRVGVG
                     QFGTTVRVIYKMPESTLWRELAEYDLAKETGMVPLAFDADPDWLYVRDQHQGKAAIFK
                     MNVADRRVERLLVAADAKHDLTGELVYAPGRKKVVGVRYSGADERVLFWDFDAQRLQA
                     RIDRAIPGRVNVIHSSSDDGRLHIVKSSGAAHPPQWSLFDEHDGRMVLLGKSYPDLEA
                     AGLVSSRETFITARDGKEVPAFLTLPKDRDPRELPLIVFPHGGPASQGRDAFNYWTQW
                     FVSRGWAVLAPRFRGTEGYGDELLRAGFQRWGLEMQDDVTDTVQWAIRSGLASAHRIC
                     IVGSGYGGYAALMGAVKTPDLYRCAVSLGGVTDLPQVVSDSRWYLNQKPLAELRIGSW
                     WNDRERLRDTSPVFHANEIRLPLLLMHGAMDRAVPVSHGRDMAEALKAANVMSYRYVE
                     LPFADEALRREEDRLQIFSEMERFLTQYLD"
     gene            13283..13708
                     /gene="exbB"
                     /locus_tag="PK224_16405"
     CDS             13283..13708
                     /gene="exbB"
                     /locus_tag="PK224_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HRB17378.1"
                     /translation="MEALKAGVEYGVIGLLGLLAVWALVVAIERWRFYGRVDPSRFAT
                     VQTFEMALTKRLVIIGTIAANAPYIGLLGTVLGIMLTFHTMGTSGAMAVTTIMIGLSL
                     ALKATAAGLVVAIPCVVMNNVLRRRVSELLAQYKAHHGA"
     gene            13698..14072
                     /locus_tag="PK224_16410"
     CDS             13698..14072
                     /locus_tag="PK224_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRB17379.1"
                     /translation="MEREVDQINVIPLVDIMLVLLVIVLTTATFITTGQIPVNLAKAS
                     TSSDRQDRPIVVTVTAEGTVFVEDRAITDEQLDTALATHSRQSPVLVRADKVTRLERF
                     VAVVDRIRGLGFQQVSLEVMKW"
     gene            14221..14619
                     /locus_tag="PK224_16415"
     CDS             14221..14619
                     /locus_tag="PK224_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17380.1"
                     /translation="MNEFLRAMHATDRSGRTPRLVALLACLLIGIRFAASIVCSTCFT
                     DWEQAKTRAFYLHSGWDRDRCHHGLAPASLWIVWACSVNEDESAFVLPEIPRLPVLVS
                     IFVPLLFLLISYRSLIPITANGRGPPLNAC"
     gene            14845..17193
                     /locus_tag="PK224_16420"
     CDS             14845..17193
                     /locus_tag="PK224_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HRB17381.1"
                     /translation="MKSVESVQEVRRPCRSLWPVGRIAAACAIMMTFTACIGPHKPVP
                     TQPAQEPAAQSAGTAEPSPAPDAASAEAPPSGVPLVPLPVPPIAEAPAEAPASTAGDV
                     PVVDVKPVYVVAQHESYKVDQATTATKTDTPIMETPFAIQVVPQQVLRDQQTTRLDHA
                     LNNVSGVFANQSLSLFESFTIRGFETFDYYREGTRFQSALTQTGRREMANLERIEVLK
                     GPASILYGRIQPGGMINLVTKKPLEQSYYSIQQQVGSYDFYRTALDATSKLNESGSLL
                     YRFNMSYESKGSFREFIDDKKLFIAPVVQWKLSERTQITFDGEYSKGKVRPDYGTVAL
                     GNRPASLPIERNLGESFAKADYTALLGGFNWSHEFNSQWKIQHRAYLQSTKEDDQVVL
                     PLALQADNETLDRFFAGFQGNKHKTYTTSLDLTGHFDTFGLKHRLLAGADYYQFKNTA
                     TMVDNFAFPSINIFNPVHGGNPIADPADNFEFDLREKWFGLYLQDQVELPFHLHLLAG
                     LRYDSAEITTDNTFGGTRTISSSRQSAVTPRVGLLWQPIKELAFYGNYVEGFGVPNVG
                     SLGVTGAPLRAETSQQWEAGIKTELFDGRWTATLSWFELTKQNVATGHPDPVLAALGF
                     SVQTGEARNKGVELDVAGEVLPGVDVIATYAYTDSEITQMNDGTIGNRFPNVPKHAGS
                     LWTTYRFQEPRLQGWKVGAGLVARGERQGNRENDYQMPGYVLVNLMASYTMHVGATRL
                     TAQVNVDNLLGQEYFPSSAGFGRGRIDIGTPRFFLGSLKMEF"
     gene            17371..17760
                     /locus_tag="PK224_16425"
     CDS             17371..17760
                     /locus_tag="PK224_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17382.1"
                     /translation="MLRHLHSHRRSAQASRAVVLLASLLIGIRLAAGVIHAACYVDWE
                     KPNARSFYLHSGGDHDRCHHGQVPPDPWAEWGCEASQDEPGFTLPEIPRLPPLVSWFA
                     PLGLFLISCRSFSLIAAHGRSPPVPHS"
     gene            18005..20302
                     /locus_tag="PK224_16430"
     CDS             18005..20302
                     /locus_tag="PK224_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HRB17383.1"
                     /translation="MARRLLLMLSCLTVLTACIGPHKPVPTQRSQSAQVPTEAEPARE
                     HVNGEAARTEPDSMGPVVPLPMPPIARTPAEVSGTPSAQAEVPVVDVKPVLVIAQSEP
                     YKVSQANTATKTDTPIMQTPFSVQVVPQQVIRDQQAVRLETAVQNVSGVIVQRGANGD
                     TSDSFVIRGFNVDHTYRNGVLGGGSFGQGHREMANVERVEVLKGPGAILYGRSEPGGI
                     VNIVTKQPLATPYYSVQQQFGSYNFYRTTADATGPLTKDDTLLYRVNVSYENSGSFRR
                     FMKEDNLFIAPVVQWNISPRTQITAEFEYLHFDRTREFGTPVIGNRPAPIGRNDTVVD
                     PGFNQNLGDRYFAGLHGSHRLNADWEASLRLSLTQLETSVDNNLFFNLAQPSGDLERF
                     YFNGPTTLRNYQTTFTLTGHINTGVAKHTLLAGYDFFYLSTKFSGDNAVSAPPFNIFA
                     PTFSNPAPVFDQGSFSGSTAETWHGLYLQDQIELPYHIHLLAGARYDNTRGTDNQLGV
                     TTYEEDRLSPRGGLLWQPLPWLSLYGSYTQSLGPSNVFFRTDGVKMRPQSGQQWEAGV
                     KTEFWDGRLRMTAAYFDLTKQNIAVPDPANLTRLRPVGEAQTRGIELDITGEILPGWQ
                     VIAAYSYLPFAKITHDVADDGAGNPTAGGTGNRLFLAAKDSGSLWSTYEFQDEALRGL
                     KVGGGVVAVGERQGDVANTYQLAGYVTANLMASYQWHVGMTRLTAQLNVNNLFDVSHF
                     AGSNAFDSAVFGMPRFFMGSIRMEF"
     gene            20478..20876
                     /locus_tag="PK224_16435"
     CDS             20478..20876
                     /locus_tag="PK224_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17384.1"
                     /translation="MNDIFRLISLRGRTGRTPSLLLLLACLLVAIRLAAGFVCVTCFT
                     DFEKPTSRSFHLHAGGDLNPCHHGRVEAGPLINWACTVTQDDTAFILPDVPLLPVVVS
                     LFVPLLLLVVSYGNRLLIAAHGRSPPPVIA"
     gene            21059..22276
                     /locus_tag="PK224_16440"
     CDS             21059..22276
                     /locus_tag="PK224_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRB17385.1"
                     /translation="MAQEIAAPASLAGNQAGKTQARPVWKYVWRTWWVQVHLYLGLSV
                     GALLVVFGVTGSILVFFQEIDEWLNPVLLTVSVPSTGSAPVRPLQDIVTAAQQAATPG
                     SRIVQLYGPRNRTGVFAIYAEHPSKAWQRIYIDPYRAAVTGVRSYAAYEWVPHYLMDV
                     VFQLHFALFLGENGLIIVALGALLLLLSLISGVIVWWPLTGQWRQAFVLRWPSTPVRL
                     NFDLHKTFSLYTCLVLGAVLLSGVWMNWNDPFVWVVRLLSPATRGPKQAPVSIPLEGR
                     QPITPAQALAIATARYPEGVLNILVMPEDSTGIYLVGRREVPGLSLFWSERTVSIDQY
                     SGAILDVREPATRRSAGETFADWQWPLHSGQAFGLPGRLMVCLSGLACPVIYVTGVRM
                     WWRKRSARRMRPA"
     gene            22342..>22784
                     /locus_tag="PK224_16445"
     CDS             22342..>22784
                     /locus_tag="PK224_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRB17386.1"
                     /translation="MSAQPTIEQPEPRKPSIHDADLDGLAIRRTEPHLPRRNGRGRWW
                     LWLLLLTLVTGGAFVAWKSGLTGYGETVEVTPVVRMPAGGVSGLAATGYVVAQRQALI
                     ASKGTGRLEYLGVKVGDPVKEGQIIARLEHSDIDALLQQMVAKLNV"
BASE COUNT         4445 a         6399 c         7296 g         4644 t
ORIGIN      
        1 agcgaagggg cgaagcgtac tgctcccgta cggtgagtct ctgaacgttg cgagaacgaa
       61 gctggcggac tttttcagca ttctgcagga gcaaggggag gagtcatgaa gacgcaggtc
      121 gatcagcggg tgggacggat ggggctgtac gggttggtgg gattggcgat catgctgtgg
      181 gcgccgggcg tgtccgccgc cgatggaaag gcggagaagt cgttcaccat tgccgtgacc
      241 gaccagcagg gggtcgagac cgagttgaag aacggcatct tctattggga agaaaaaatg
      301 agtgagacct cgttcgtccc gcatgagttg cggcacctgc cggccaagcg cggcacggcg
      361 acggtcaata tcaagttcga ccagatcaag cagattgaga tcaagcccgg tgccgacaag
      421 actgcgccgg ccatgacggt gaccttaacg aacggcaaga acggggaatt tacgcctgcc
      481 gtcagcggca gcttcaaggg cgagtcggat ttcggccagg tcgaggtgcc ggtcgggtcg
      541 gtgtccaagg tgatcttcaa gtaagggcgg aacgaatcgt ttcgaaaggg agtgtcccat
      601 ggagaatgtg ctcgtggcgc aatcgtggca acccaagcgg tcgatgacag ggtggggagc
      661 gtcgctcctg ctgcacgcct gtctcgcgct cgctgcattc ggtctgatgc cgaagatggc
      721 catggtggtg gagaaagagc cgtttaaatg ggacgtggcc ctcgtcgagg ctccgcgcga
      781 agtcgtgcgc caggaagagc cggctccggt gccgcaggtg cagcctcccg ttccgactcc
      841 ggtgaagccg cgacctgagc cggtacgtgc cgtgccaccg ccgcctcagc cggtgcaacg
      901 tcaggtggaa acgcgggtcg tgccgcaggc gatccaacgt gaggtgcagc cggtcgtcga
      961 aacggttcgt cctcaagaac agattcagcc ggccagggaa gtggtgcagg tgcaagccaa
     1021 gcccatcgag ccgcaagaga ttcaaaaaac ggagccgttg atgcaagcgg cggtgccggc
     1081 tgaggtggta cgggaaaccg ctccggcagt cgaacagacg gttgtggagg cggcgcctgc
     1141 aacggctcag acctatcagg cccaggcggt ggtcagcgag ccgacggtga tggagacgag
     1201 gcccgaaccg gttgttacgg cgagtgcgcc ggtcgtgcac gctgctccgg cggctgaacc
     1261 ggccccggcg agtcccgctc cggccgtgga ggccgtcgcg tccgtcagtg agccggttcc
     1321 tgctcctccg gccccggtcg ctgccgtccc cgcgccagtg gcggcagcgc agccggatcc
     1381 agcgacgtta caagaacatc aagtcgtggc gcgcgtggcg acgccgaggc ccgccaccaa
     1441 agccgattat ggctggttgg ccgaatcgct gcatcgccgc atcatcgaac tccggcatta
     1501 tcccagcacc gcgcgcttga acggctggga aggcaaagtc gtcttgaagg tctcgatccg
     1561 ccaggacggc caactcaaag acgtcgaggt ggtgaaaagt tcagggcatg agtcgttgga
     1621 tcaggcggcg atggaagcgg tgcggcgcgc ctgtcctctg catatgaagc acgagttgac
     1681 ggcgccgatg gtcgtgctgc atctgccggt cagttacagt ttgaatcgct aggccgcctc
     1741 gctggatgac catgttgacc atcctgcgga ccgtccggga aagagtaagt cgctgatcat
     1801 ggatagagta agcaaagatc tgcgaccggt cggtttcccg attcttgtct gtgcggggat
     1861 tctcgcggcc tggtggattg ccgcgggctg tgtgtgggct ggagatgggc aggccgtcgg
     1921 ttccgattct gctcgggctc agcggatcga ggaacttcag aagcatcggg agcgcattga
     1981 gcgggaattg aatcaattga aacagcagcc ggacggggtc agccgctcgt cggtgccgag
     2041 gaccgaactg agcaaccagc ccacgcggac gctgaaagaa tccatggagt cggttccggg
     2101 ggttgcgtcg tctccgggag cagagggacg atccgtcgat ctctccatca gaggagccgg
     2161 aaaatgaatg tgggaaggaa agggcagtga tgggacagtc tgaaacaagc ctgggcggtc
     2221 tgttggccct gtgcctcgct gcggcgatcc ctattgcggc cgcgcaggcg gagtcggccg
     2281 ttcccttcga actcagccag aagtttgtga ccgaacacaa ggtgaagagc ctggtgggtc
     2341 cgtccgtgca ggtggatgag caggggcaag tctccttggc ctggatggaa gaggagaagg
     2401 atgtgcggtc ggttctgtac gcccgcagcg cggaggcagg cgggccgatg ggagtgccgg
     2461 tgcgcatcaa ccgcccggag gacgtgccct attggcgcca ggaagcgccg gcgctggttg
     2521 tgcaggggga agacgtgttt gtcacctggg gcctggccca tccgaaagcc acgccgcaac
     2581 agcccttcgc gacggaattg cgattgagcc gctcgatcga tgggggcaag acgtttcagc
     2641 cctccgtcgc ggtgaacgac gatccggggg tcattcaaca taccttcgat gcgttgcatc
     2701 gtgatgcgga tggacggttg cacctctcct ggatcgacgg gcgtgaagga aagaaggagc
     2761 cgggtaccta cgtggcccgt tcgttggacc gtggcgcgac gatcaccagg aatctcaagg
     2821 tggacgaggg tacctgtgtc tgctgccgca cggcggtgac gagtggtcct gagggcatgg
     2881 tctacgtagc ttggcgcaag atttttgaag gcaatgttcg tgaaactgtg gtcgcccgtt
     2941 cgacggacca cggggagacc ttcgaggcgc ccgtgatcgt ggggcatgat cgatgggtgt
     3001 tcccggcctg cccgcatcgt ccggcctcga tgggtgtcga ccgtcagggt cgtctctacg
     3061 ttgtctggta caccgaagga acggacgaaa ttcctgccgt ctacatcgcc tattcggacg
     3121 atcgagggaa gagtttttcc gaaaagcgga agttgaacgt ctcgaaaaac acctttcccg
     3181 accatccgca gattgcggtc gatcccgaag gacggatcgt ggtggtgtgg gaggagcagg
     3241 ctccggtcaa gcgggatgtg gtgatgagcg tctcgatgga tcgtggggca acgtttacgg
     3301 cgccgcacaa ggtaaacgag aaaaaaagcc agactccggt cgtggcggtg aatagtcagg
     3361 gcctgtttgc cttggcctgg atggagcatg gcatgccagg tcataaaatt gtgatgcaga
     3421 ccgtgaaggt gccttcggtg aaggtggcgg ctgaaccggt gcgttagctg ggtgggtttg
     3481 tgattggaac gatgatgccg acgggattgt tgagaggcct catgctggcg gttgtgctga
     3541 gcacgacggc cggtcctgta tgggcggatg atcctttcgc cgcgttgaaa atcgcgcggg
     3601 ttgcaccggg cactgccgct gcggcctttg agctacagtc gctggatggc cggtcggtgc
     3661 aactcgcgga cttgaagggg aaagtcgtcg tggtgaattt ctgggccacc tggtgcgggc
     3721 cctgcaaaga agaaatgccg gcctttgagc gtctgcgacg gcagctggac cccgaccgct
     3781 tcgtgttgct gaccatcacg accgacttgc agcgcgccgg catcaagcag ttcctggcta
     3841 atctcaacgt gcaactcccc gtgttgttcg acgagcagca ggatgtctcg caagcctatc
     3901 tggtgcgggc cttgccgacc acggtgttca tcgaccggca gggggccttg ctgggccgcg
     3961 ctgtcggacc gcgtgagtgg gatgcgccca aggcggtgca ggcgttgcag gggctgatgc
     4021 catgagccgt ctcaagacac cctccgtcgg actcgcggcc gcacttgcgg ggatctatct
     4081 ggtcctggcg gcgttctcca tggcctgcgc cttcgaccat gccgacccca tgtcggccgg
     4141 ccatcatcat ggcggaacgg tctctcactc cagtttctgt aattgggctt gtcaggccaa
     4201 tcccacgtcg gatgctgcgg cgtccgcact ggtgcttcat ccgttccttg tggtcgcgct
     4261 gtttgtcgag cgcgatcata ccgtcatcgc cggcggcgcg ggcttccacg ccgcctctcg
     4321 cgcgccgccc gttcaatcgt aattccattt cagatcgcta ggtgttctag cagcttttct
     4381 cgtccggcca gacaggggcg ggaagggtat gtctctgctg tcgtgtgcac agttgtgccg
     4441 ggaggacgtg tatgtgggcc cgtcggaatt catctcaaat tcaattcatt ctgctgttca
     4501 ccctggtagt ggcactgccg ttcacagcct cccgcgcact tgcgcaggag gaagcggcgg
     4561 ccggcgcggt cgttgaaaaa agcgcgcggg agcgggactt gagggatcag ctacagaaaa
     4621 ttcttcatga actggaggaa ctccgacagg cgcctgcagg tggcgcgacc gccgcgccgg
     4681 tacagccgtc ggtggtgcag gagccgtcgg aaggaaaagc cgccgaagcg attccgcaat
     4741 ttgaactggc cgatgtcagc atcgtgagcg accgggtgca gcggcgtccg gaagggatca
     4801 cgctgtcggc gacggagcag tcggaaaccg aatcgcagcc gaccaggacg cttcgtgagt
     4861 cgatggagtc gctccccggc gtgatcctgc gacaggccaa cggaccgagg gatttcagta
     4921 tttcgatccg tggctcgggc gtcaaaacct cgtttgcggt gcgtgagatc aaaatgtacg
     4981 aagacggatt cattcaaacg caatccgatg gattgtcccg tctggatatc cacgatccct
     5041 ggtttatgcg gagcgtcgaa gtcacgcgag gcgcgtcttc ctctctctac gacaactacg
     5101 cactgggcgg catggtgcac ttccgtacga ggcgtggcag tgacatcaac gggatggaga
     5161 gtttcatcag cggcgggtcc tacggctatc agaaatacgg attcgccgtc ggccagcaat
     5221 tcgacaatgt ggacatctcg ctcttcggca gtcacgtcgc cgaggacgga ttcattcgcc
     5281 acagcgacta cagcacgcaa acgatgaatt ttaattttcg cgtcaaggtg gacgacaagc
     5341 agaacctgta tttcaaggcg attacgaact ggttggactc gcgggtgccg acgcggctga
     5401 cgctgtcgca gttcgatgcc gaccaccggc aggccggcgg catcaacaat gcgacgaatg
     5461 cggtgctatt gggccagcgt cgattggatc ggcggaatat catcggtgga atgtatgaac
     5521 ggcagatcga tgccaacacc gtcttgacga tggaagccga ctacgacgtg aaggacatca
     5581 atcagacctt cacgcagatc agcgacaacg tcaatcccaa ctacaagcac tatacggact
     5641 tacgtcacga cggacggctg ttcgacatgc cgttgcgcag ttacgttgga ttttttgtga
     5701 atcagatgga gcaggaaggg aacaccttca ataacctcca ggacttcttc ggtacgcgcg
     5761 gcacgttagc ccagaacagc cgcgggacga tccggaacat cggcggacgg ttccgggaag
     5821 aattggaatt tattccgaag tggaccctgg ccgcaggcct cgggttcgag caatcgcagt
     5881 tgagcgtgca gacgatcaac tacacggccg gggcggtgag tggccggtcc ggagccgaac
     5941 gcagtttcag caactgggcg ccggaactgt cggtcagctg gaagccggcc gacggatatc
     6001 gccattgggt gcgtgcctcc accgggtatg ccattccggg gttcggcaat ctgttgacca
     6061 atccctttac gggtctcgcc ggaactaact tcgatctgaa gccgcagaaa aacctcaatg
     6121 tcgaaatcgg tacggaatca cgcctgcaca agaccttgac ggtgcagttg gtcggcttcg
     6181 tcacgttcgt caaagacgaa atcatcaccc aagtcgtctc caacaccggc ggtcaggctg
     6241 cgatcaacgc ggactcgtcg cagtatcgag gcatcgagtt gttctacgac tggcggccgt
     6301 tggacgggtt gcggctctcc ggcgcctaca cccacatcga tgcgcggtac atcaatttca
     6361 gcgaccggtt cctggccggc gggattccga cagcgttcgt gcgggacggc aaggcggtgc
     6421 cgaacgtgcc gacggacacc atcaatttca aggagtctta cgatcatgcc gccagcgggt
     6481 ggggcggctg ggttgaagtc aactatatga acagttactt cctgaacaac aacaacacgg
     6541 tgggtatccc cgcctatgtc atcgccaacc tgaatatcca taagaacttc gagttccata
     6601 acaataagtg gatccggttc gcgaagttct tcgcggagat cgacaatatc gcggacaaga
     6661 aatatgcggc ctccggcaac gtggtggccg atagcacggc cgatgcgaac aagacgttgt
     6721 tcttcgccgg ctatggccgg tcgttttatg ccggggtgac attgggattg ttctaaccat
     6781 atggcgcgag cggtgcccgg atgcggggcc gttcgcgccc ttaacaagga gattgccatg
     6841 aacaagcaac tcttgcgggt cggtgaggcg gcggatgtat tggcggtgag ccgttggacg
     6901 atttatcggt gggtggagga agggcggttg gagggcacca agatcggccg cgggagcctc
     6961 agggtgttcc gcacgtcgct ggaccggttg gtgcaaaaca ataagacgca ggaaatgaac
     7021 ctgaccgtct gatgcgtgtc tcgcaaaggc tgcacctggc ccgtgagagg tcgatgcggg
     7081 cttgtgtgcg cgagtgagag gacctatgca tcgcattctg ttgtgtcttg tgtttctgct
     7141 ggccggttgc gcgggaggga cgggcctatc ctcatcgtcg aaggcctcgc agcagaccgg
     7201 gccgtccggg agcgattcct acgccttcat caacggccga tggttcgacg ggaaagggtt
     7261 tcaggcggcc acctggtatt cagtgcaggg acgtttgacg cgcaccccgc cgcaggggaa
     7321 ggtcgaaacg gtggatttgt ccggcctgtt cgtcgtgccg ccgttcgggg aagcgcacaa
     7381 tcataacgtg gaaggtccgt ggaatgttca ggcggtggtc gagcggtatc tcaaggacgg
     7441 cgtgttctat gtcaagaatc ccaacaacgt ccgggacttt gcattacaga ttcgaagcgc
     7501 cgtcaatcaa ccggcgagca tcgatgcgac ctttgcgcac gcagggctga cggggcaagg
     7561 aggccatccc atcgcgttgt atgaggatgt gttgcgcctg ggccggtacg aaccggccat
     7621 cgggacggtc gagcggggct ggtttgagaa ccggtcgtac atcgtgctcg acaccgatgc
     7681 cgatgtggag acgaagtggc cggtgatcac gagtggccgg cctgacttcc tgaaagtgta
     7741 tctcgtgcat tccgaagacg atgacgcgac cgggaagtcc ggacattccc cgcagcgaag
     7801 cggtttgcat cctcgactcg tggcacagat tgtcgccaag gcccatgcgg cagggctaca
     7861 ggtgacggcc catgtcgaaa cggcggcgga ctttcgccat gcgatccgtg cgggagtcga
     7921 tgagattgcg catgtaccgg gatggttggt gaacagtgct gccgatgcgg agtccgcccg
     7981 actcacggaa gcggatgcgc ggttggcctt ggaacgtcac gtgcgggtcg tgaccaccgc
     8041 tgtggcggga agcgccatgc cctccgtgac aggacatcac ccgcacgggc actacgaagg
     8101 gcatgccacc gggccgtccg atcatcacgc aacggcgccg gatgaatcat tgtcacaggt
     8161 tctcaaggac aacatcgcgt tgctccatcg cgtgggagtg cgcgtagtgg tcggcagcga
     8221 ccatgccgag acatcgctgg cggaagtgat gcaattgcac aggttccatc tcttcgacaa
     8281 cctcacgctg ctcaagatgt ggtgtgagga cacgccggcg gcgatttttc ccgaccgtcg
     8341 gatcgggcga ttcgaggagg ggtacgaggc cagttttgtc gccttggccg gcaaccccat
     8401 cgaggatttt tcacaggtgc agcgcatcgt gcgacgattc aagcagggtg tgaatctcga
     8461 tcgttccttc cgtcatgacg ccgccccttc aggggccggt catcgggctc actgacaagt
     8521 caggcggcct ggactggtca gtcgatataa tcgtgtcttt tttatttatt tttgcaggag
     8581 gagttgagcg atgaaggcag tggtgtggtg gggaagagga acggcggcgg cctgtctggt
     8641 cgcggccttg atggtgggga tcgaaccgtc tgttgcgcag gcggccgggg agccgtcgga
     8701 tcaggaggcg ccggttgtcg tcgttgatcc ggtggaggtg aaggggaagc ggattgaaaa
     8761 cgtggaggat gtgaagcagg aacttgcccg ccggccgggc agcaatatcc tgatcgagga
     8821 aaagcagatc actgagtcac gcgcgctcaa cctgcaagac gtgttgcagt tcgcaccggg
     8881 cgtacggttt cagtcgcgtt tcggtgccga tgaagggcag ttccaaattc gtggcacctc
     8941 gttgcgcaat aactttcacc atcgcgggat caatattctc atcaacggaa tcttcttcgg
     9001 cgatgccgac ggattttcag acttcgaatc gatcgacctt ttggcctacg aacgcatcga
     9061 agtttacaag ggcgcgaacg ccctgcggta cggcgcgaac agcatcggtg gcgcgatcaa
     9121 tttcgtgccg cgcaccgggt atagcgcctc caccctgcaa atgcggatgc tcggcggcag
     9181 cttcggcatg gtgagcggcc aggtgtccag cggcaaggtc ttgcagccct tcaaggtagg
     9241 caacatgagc gcgacgatgg attactacat cagcgtgtca ggcaatcgcc aggacggata
     9301 tcaagacaac agccagcagg cccgggagcg agtcaatgcc aacatcggcc tgcagctcgg
     9361 caaccaccag gagatccgcg cctactttct gcaggcgaat gtggcggagc gcattcctgg
     9421 atcgttgacc acgggacagc tctttcaaaa ccggcagcag gcgggcgggc agagccctcc
     9481 cggcaccccg ccgttttttg cgtgtaatca gaataaccaa gtctgtaact acgggcggta
     9541 ctataacttg cagcgtatcg gaatcgcgta ccacaacgag ttcgccccga accaatattt
     9601 tgagatcatt ccctacttct cgaaccagta tgtggaccat cctatctttc agacgatccg
     9661 acaggagaac aataacgtcg gcggtgagtt ccggtacgtg aactccaact cgttgttcgg
     9721 taagaacaac tcgttcgtcg ccggattcca gccgcgatac ggcaatcagc ggcagcagcg
     9781 gttcgtgaac atcaacggca gtatcggggc gctgacgcag aactatacgg ccaagacgac
     9841 gtatttcggg atgtatgcag aagatgcgtt tgatgccacc aaggatttca ccatcgtgct
     9901 cggcggccgg tgggattaca cagggcgtca ggcgacggtg gataatttcg ggccggccgg
     9961 gaatcccttc aatcccaata cgccgtccac gccgaccggc acgaaccggc cgttacagca
    10021 tttcgatgca atcagcccga agatcgggtt cgtgtatcgc acaacgccga cctcgcaatt
    10081 gtatttcaat gccagccggg cctatgaggc gccgctcaat ctggagttgc tctcctcggt
    10141 taatgcgaac ggcacgccga atacaggatt cttgaacctc gatgctcagc gggcatggca
    10201 attggaactc ggtcaccgag gaacatcagc ggacaagcgc tacacctggg atgtgaccgt
    10261 ctacaacttg gaaatgcaga aggaaattct ggcctcgatc atcaacaacc aaggaacgtt
    10321 ccaaaatgcg aacggcacgc ggcataccgg cgtggaagcc ggtggcggga tggtcttggg
    10381 gaaaggtctg ttcgcgcaag gcggagccgg acgagaggac agcctgcaga cgcgtgtggc
    10441 ctacacctgg tcgcgtttca aattcaccga cgatgtgcgg ggcggcggag cggtggggcc
    10501 gaatgtgctg atcgccaagg atgggaatac ggtcgccggc gcgccggaac atagcctgaa
    10561 cgtggagttc cggtacgacc atccgtcagg ctggtggatc gcgccgaata tcgaatggtc
    10621 gctgtccggc ttttatacgg actatcaaaa caccatcaag aacccctcct atgcgcttat
    10681 caatctgcgt tctggatgga acgtcgatga acactggacg ctctttgccg aaggacgtaa
    10741 tttgaccgat aagacctatg ccggcgcggt ggtggtgaac gatcagttca atcggtatgc
    10801 caacgcggga ttgggcatca gcgcgtttgg aggagtcgag tacaaattct aaccgctgtg
    10861 gaagcaggct gcaggcagcg tgctcgcatg gcacgcaggg gcccatcgtc ggatgggctc
    10921 ctgctgcacc gttcgcgaaa ggtggtgaat cagccacgca tcgctcctgg cggggtgatg
    10981 ctgctcgaaa aatgggatag acgatcgtcg tccgaacatg ggataaagaa agccgacggg
    11041 tgttcccgga tatggacatg tcctggtaac ttcagggata gaccgtgccg gccatgtacg
    11101 gacttttcat tttttttgtg cttgtctgga gcgtggtacc ggtctatgcc gattccagct
    11161 ccgggcctcc atctgtcgaa gccttcgcct cacttccccg tatcgcctcc atccaactct
    11221 ctccctcggg tcggcacctg gccgtgcttc gccaccacga gggaaagacc tatctggaca
    11281 cccaaaccgt cacggggcag gatgcgcatc gcgtcgtcac aacggacaac caggaatatg
    11341 tcatcacctg gttccgatgg gccaatgacg aacgggtgtt ggtcagcgtg cgttttgccg
    11401 attcgcgtga gggtgtcgat tcacaggaaa cccgactgtt ggcggtgaac cgggatggaa
    11461 cgcaacagaa cggcaatctc ctccgggtga gttcgttccc ttcgatcttc gggaaaaaac
    11521 actttccgca attccaggat cgcctggtgg gcaccattcc gggcgatccc aggcatgtcc
    11581 tgatcgcgct cgatatcgaa cggccgttgt ctccggatgt ctacacgatg gatgtgtatt
    11641 ccggcgagcg gacgctggtg caaaacaatc caggtctaca gcctgggcca cggaatgtat
    11701 tgcagtggat tgccgatcgc gaaggccggt tgagggtcgg agtcggccaa ttcggcacca
    11761 ccgtgcgggt gatttataag atgccggaat ccacgctctg gcgtgagttg gcggagtatg
    11821 acctggcgaa agaaaccgga atggtgcccc tggccttcga tgccgacccg gactggctct
    11881 atgtgagaga tcagcatcag ggcaaggcgg ccatcttcaa gatgaacgtc gccgatcgtc
    11941 gcgtggagcg tctcctcgtc gccgccgatg cgaagcacga tttgaccggt gagttggtgt
    12001 atgcgccggg acgcaagaaa gtggtcgggg tccgctacag tggggcggac gagcgggtgc
    12061 tcttttggga cttcgacgcg cagcggctgc aggcccgcat cgatcgggcg attccagggc
    12121 gcgtcaatgt gattcacagc agcagcgacg acggccggct tcacatcgtg aaatcgagcg
    12181 gcgcggcgca tcctccgcaa tggtcgctct tcgatgaaca cgatgggcgg atggtattgc
    12241 tcggcaagtc gtaccctgat cttgaggccg ccggattggt gtcgtcgcgg gagacgttca
    12301 ttacggcgcg tgacggaaaa gaagtcccgg cgttcttgac cctcccgaaa gatcgagacc
    12361 ctcgtgagct tccgttgatt gtctttcccc atggaggacc ggcgtcgcag ggccgcgatg
    12421 cgttcaatta ttggacgcag tggtttgtga gccgcgggtg ggcggtgctg gccccccggt
    12481 ttcgagggac cgaggggtat ggcgatgagt tgcttcgagc cgggttccag cgttgggggc
    12541 tggagatgca ggacgacgtg accgatacgg tgcaatgggc gattcgatcc ggactcgcaa
    12601 gcgcacaccg gatctgtatc gttggatcag gatacggagg gtacgcagcc ttgatgggcg
    12661 cggtgaagac acccgacttg tatcgttgcg ccgtcagtct gggcggggtg accgacttgc
    12721 cgcaggtcgt gtccgacagc cgttggtacc tcaatcagaa accgcttgcc gaactgcgta
    12781 tcggttcctg gtggaacgac cgcgaacggc tccgcgacac gtcgccggtc tttcatgcga
    12841 acgagattcg cctcccgctg ctcctgatgc acggcgccat ggaccgtgcg gtgccggtgt
    12901 cgcatggtcg agacatggcc gaggcgctga aagcggcgaa cgtgatgagc tatcgatacg
    12961 tggaattgcc tttcgctgat gaagcacttc gccgagagga ggatcgtctg cagatattct
    13021 cggaaatgga acgcttcctt acgcagtacc tcgactgacc acgctttctc catcctctac
    13081 gctgactcgc agagccattg ttgtcttcat cccagtctcc cattcctgtg tttcccttcg
    13141 aactgtagca gccgtgagca gctgccagta gacggcctgc gcctcccgtc gtaacgtacc
    13201 gccgccggta gcaacccgtg tgccgcatct cggctgagaa tagtgccggc aatcgataaa
    13261 aaacagtgag gacggcagaa cgatggaagc gctgaaagcc ggagtggagt atggggtgat
    13321 cggtctgttg ggacttctgg cggtgtgggc cctggtggtc gcgatcgaac gatggcgatt
    13381 ctatggtcgg gtagatccgt ccaggtttgc cactgtgcag acctttgaga tggcattgac
    13441 gaagcggttg gtcatcatcg gcaccatcgc ggcaaatgcg ccctatatcg ggctgctcgg
    13501 gaccgtgttg ggcatcatgc tcaccttcca caccatgggc acgtccggcg cgatggcggt
    13561 caccaccatc atgatcggct tgagcctcgc tctgaaggcg acggcggcgg ggctggtcgt
    13621 cgcgattccc tgcgtcgtca tgaacaacgt gctccggcgg cgcgtcagcg agctgctcgc
    13681 gcagtacaag gcgcaccatg gagcgtgagg tcgatcaaat caatgtgatt ccgttggtcg
    13741 atatcatgct ggtgctgctc gtgatcgtgc tgaccaccgc gacgttcatc acgacgggac
    13801 agatcccggt caatcttgcc aaagcctcga cttcgagcga ccggcaggac cggccgattg
    13861 tcgtgacggt cacggcagag ggcacggtct tcgtggagga ccgggccatc accgacgaac
    13921 aactggatac tgccctggca acccattcac gccaatctcc tgtcctggtc agggcagaca
    13981 aggtcacccg actggaacga tttgtcgccg tcgtcgatcg catccgtgga cttggattcc
    14041 aacaggtgag tctggaggtg atgaagtggt gagatgtcac gtgtccgaag gtcgctcact
    14101 ttaaaccttt cgcgtctgac gtttcagggt ctgaaactgt agtatccgtg agcaggtgtc
    14161 tgtagaccat ttcctacgcg gtgtcgtagg ctactctcct tttttctgta ggactattcc
    14221 gtgaacgagt ttcttcgcgc catgcatgcg accgatcgat ccggccggac cccccggctg
    14281 gttgcgctgc ttgcctgcct gctcatcggc attcgcttcg cggcaagcat cgtctgctcg
    14341 acctgcttta ccgattggga gcaggcgaag acgcgggcct tctatctcca tagcgggtgg
    14401 gatcgtgatc gatgccacca tgggctggct ccggccagcc tgtggatcgt ctgggcctgt
    14461 tccgtcaacg aagatgaatc ggcgtttgtc ctgccggaga ttccccgctt gccggtcctc
    14521 gtgtcgatct tcgtcccgct cctgttcctg ttgatcagtt atcgtagtct cataccgatt
    14581 acggccaatg gccgcggtcc tcccctcaac gcctgctagc ccggtacgta acaccgtcac
    14641 aacgaaacac aagccgccat cggttgagtc gtgggccatg tctgtgccgt ggcgctcatg
    14701 tcttgggata tgagtgggcg cgctctgctc tcagatcgcc gccgtttccc cgcaggcccg
    14761 gtcgcggact cggtaccggt ccgccggcgc tatgtcagcc tgtcattgcc gcatgccggt
    14821 gtgaacaggg aggagagtct tgtcatgaag tcagtcgaat cagtgcaaga agtacgtcgc
    14881 ccttgtcgaa gtctgtggcc ggtcggtcgg atcgccgcgg cctgcgccat catgatgacg
    14941 ttcacggcct gtatcgggcc gcataaaccg gtgccgaccc aaccggcgca agaacctgcg
    15001 gctcagtcag caggaaccgc agaaccgtcc ccggctcccg atgcggcttc cgctgaggcg
    15061 ccgccttccg gagtgcctct tgttccgctt cccgtccccc caattgcgga ggctccggct
    15121 gaagcgccgg cctcgacggc gggcgacgtt cccgtggtgg atgtcaaacc ggtttatgtc
    15181 gtggcccagc atgaatccta caaggtcgat caggcgacca cggcgacgaa gaccgatacg
    15241 ccgatcatgg aaacgccgtt tgccatccag gtagtgccgc agcaggtgtt gagggatcaa
    15301 cagacgacgc gtctggacca tgcgctgaac aatgtcagcg gcgtgtttgc caatcaatcg
    15361 ctcagtctct tcgagtcctt tacgattcgg ggattcgaaa cgttcgatta ctatcgggaa
    15421 gggactcggt ttcagtcggc cttgacgcaa accggccgga gggaaatggc gaacctggag
    15481 cggatcgagg tcctgaaggg cccggcgtct attctctatg gccggattca accgggcggg
    15541 atgatcaatc tcgtcacgaa gaagccgctt gagcaatcct actactcgat tcaacagcag
    15601 gtcgggtctt acgatttcta ccgcacggcc ttggatgcga cgagcaaact caacgagtcg
    15661 gggtcgctgc tctatcggtt caatatgtcc tacgaaagca aagggtcgtt tcgggagttc
    15721 atcgacgaca agaagttgtt catcgcgccg gtggtgcaat ggaagttgag cgagcggaca
    15781 cagatcacct tcgacgggga atacagcaaa ggcaaagtgc gccccgacta tggcacggtg
    15841 gccctgggta atcgtccggc ttctctaccg atcgagcgta acctgggcga gtcgttcgcc
    15901 aaggcggatt atacggccct gctcggcgga ttcaattggt cgcatgagtt caatagccag
    15961 tggaagattc agcaccgggc ctatctgcaa tcgacgaagg aagatgacca ggtggtattg
    16021 ccgctggctc tgcaagcaga caacgaaacc ctggatcgtt ttttcgccgg attccaaggc
    16081 aacaaacaca agacctatac gaccagcctc gacttgaccg ggcacttcga cacattcggg
    16141 ctcaaacatc ggctgttggc gggcgcggac tattaccagt tcaagaatac tgccactatg
    16201 gtcgacaact tcgcgtttcc ctccatcaat atctttaacc cggtccatgg cgggaaccct
    16261 attgcggatc cggcggacaa ttttgagttc gatctccgcg agaaatggtt cgggctctac
    16321 ttgcaggatc aggtggaatt gccgtttcac ctgcatctgc tggccggcct gcggtatgac
    16381 agcgccgaga tcacgaccga caataccttc ggcggtacgc ggaccatatc gagcagccgg
    16441 cagtccgccg tcacgccgcg cgtcggtctg ctctggcaac cgatcaagga actggctttt
    16501 tatggcaact atgtcgaagg cttcggtgtg ccgaatgtcg gatcgctggg tgtgaccggt
    16561 gcgccactga gagcggaaac gtcccagcag tgggaagcgg gtatcaagac cgagctcttc
    16621 gatggtcggt ggacggcaac cctgtcctgg ttcgaactga ccaagcagaa tgtggccacg
    16681 ggtcatcccg atccggtgct cgccgcctta gggttctccg tgcaaacggg cgaagcgcgc
    16741 aacaagggcg tcgaactgga cgtggccgga gaagtgctcc cgggggtgga tgtgattgcg
    16801 acctacgcgt ataccgattc ggaaatcacc cagatgaacg acggtacgat cggcaatcgc
    16861 tttccgaacg tccccaaaca tgcaggcagt ctgtggacga cgtaccggtt ccaggagccg
    16921 cgtctccagg ggtggaaagt aggggccggg ctggtggcca ggggagagcg gcaagggaat
    16981 cgggagaacg actaccagat gccggggtat gtgctggtca atctgatggc aagttatacg
    17041 atgcacgtcg gggccacgcg actcaccgcg caagtgaatg tggacaatct tcttggccag
    17101 gagtatttcc cgagcagcgc tggcttcggc cggggtcgga tcgacatcgg aacgccgcgc
    17161 ttcttcctgg gatccctgaa gatggagttc tgagggcgtg aaacgtcaca cgtcaaacgt
    17221 gagatgtgag acgtagggag gggaagacag tctgcaccgc gagaacgcgg tgcggactgt
    17281 agcatccatg agcaggcgtc cgtagacagg tcgcccggcc ccttcatata gtgccggagt
    17341 tcttacggaa agggtgaggt ctcaggggac atgctccgcc atctgcactc tcatcgacgg
    17401 tccgcgcagg cgtcacgtgc ggtggtattg ctggcctccc tcctgatcgg gattcgattg
    17461 gcggcgggcg ttatccatgc cgcctgctat gtcgattggg aaaaacccaa cgcacgttcg
    17521 ttctatctcc atagcggggg agaccatgac cgctgtcacc atggtcaggt tccccccgat
    17581 ccctgggcgg aatggggctg tgaggcgtct caagacgagc cgggatttac acttccagag
    17641 atcccccgcc tgccgcctct ggtctcgtgg tttgcaccgc tcggcctctt cctgatttcc
    17701 tgccgtagtt tctcgctgat tgcggcccat ggccgcagcc ctcccgtccc gcactcgtaa
    17761 cgtcaatctg acacttggtg aacattcacg agccggtcgc ctacgcgatc cgatctcgcc
    17821 tgcgcgcacc gtccggtggc gacaggccag aggcggtgcg cgcgcagccg acggctctca
    17881 gcgtgattgc gcgcaccgtg aggcggtgtg cgcgagagag ggagtcatgt cgttcgaagt
    17941 cttgccagga attcggagtg ttcgaacccg tttgtatacg aatgtatggg cctggaacgg
    18001 gcgcatggcc aggaggcttc tgctgatgct gtcctgtctg acagtcttga cggcctgcat
    18061 cgggccgcac aagccggtgc cgacgcagag gagtcagagt gcgcaggttc ccacagaggc
    18121 tgagcctgcc cgagaacacg tgaatggaga ggccgctcga accgagccgg attcgatggg
    18181 accggttgtc ccgctgccga tgccgcccat tgccaggacc cctgcggagg tatcaggtac
    18241 cccatccgcc caggcagagg tgccggtcgt cgatgtcaaa ccggtactcg tgatcgcaca
    18301 gagtgaaccg tacaaggtgt cgcaggccaa caccgccacc aagaccgaca ccccgatcat
    18361 gcagacaccc ttctccgtcc aggtggtgcc gcagcaagtc attcgggatc aacaggcggt
    18421 ccgcttggag acggcggtgc aaaacgtcag cggcgtcatc gtacaacgcg gcgccaacgg
    18481 tgacacatcg gactcgttcg tgatccgcgg gttcaatgtc gatcacacct atcgcaacgg
    18541 ggtattagga ggcggaagtt tcggacaagg ccaccgtgag atggctaatg tggagcgggt
    18601 cgaagtgctc aaggggcccg gagccattct ctatgggcgg tcggaacctg gcggcatcgt
    18661 caacatcgtg acgaaacagc cgctggcaac gccctattac tccgttcagc aacaattcgg
    18721 ctcctacaat ttctaccgca ccacggccga tgcgactggg ccgctgacca aggacgacac
    18781 gctgctctat cgtgtgaatg tgtcctatga gaattccggg tcgtttcggc ggttcatgaa
    18841 ggaagataat ctgttcattg cgccggtggt gcagtggaat atcagtccgc gcacgcagat
    18901 tacggctgag tttgaatatc ttcatttcga ccggacgcgg gaattcggca cgccggtgat
    18961 cggaaatcgt ccggctccga tcgggcgtaa tgacaccgtt gtcgatcctg gattcaacca
    19021 gaatctgggg gatcgctatt ttgccggcct ccacggatcg catcggttga atgccgattg
    19081 ggaagccagt ctccgcctct cgttgacgca gctggaaacg tcggtcgaca ataatttgtt
    19141 cttcaacctc gcgcagccca gcggagacct cgaacgattt tatttcaatg ggcccaccac
    19201 gttgcggaac taccaaacga cgttcaccct caccggacac atcaacaccg gcgttgccaa
    19261 gcatacgctt ctggccggct atgacttctt ctatctttcg acgaagtttt ccggcgacaa
    19321 tgcggtgtcc gctccaccct tcaacatctt cgcgcccacc ttcagcaatc cggccccggt
    19381 gttcgaccaa ggctcattca gcggcagcac tgcggagacc tggcacggct tatatctgca
    19441 ggatcagatt gagttgccat accacattca cctgctggcc ggggcgcgat acgacaatac
    19501 tcgtggaacg gataaccagc tgggcgtgac gacctatgag gaagaccgcc tgagcccgcg
    19561 cggcgggctg ctatggcagc ctctgccctg gctctcgctc tacgggagct atacgcagag
    19621 tctgggcccc tcgaacgtct ttttccgaac ggatggggtc aagatgcgac cccaaagcgg
    19681 gcagcagtgg gaggctggtg tcaagaccga attctgggac ggccgcctcc gcatgacagc
    19741 cgcctatttt gacttgacca aacaaaatat cgctgtcccg gatcctgcga atttgacgcg
    19801 gttgcgtccg gtcggcgagg cgcaaacgcg cggcatcgaa ctggatatca ccggcgaaat
    19861 cctgcccggc tggcaggtga tcgcagccta ttcgtacctt ccgtttgcca agattaccca
    19921 cgatgtggcg gatgacggcg ccggaaatcc gacggccggt ggtacgggca atcggctctt
    19981 cttggcggcc aaggattccg gcagtctctg gagcacctac gagtttcaag acgaagcgtt
    20041 gcgcggcttg aaagtcggtg gcggtgtggt ggcggtaggc gaacggcaag gcgatgtcgc
    20101 caatacctat cagttggccg ggtatgtgac ggccaacctg atggccagtt accagtggca
    20161 tgtcgggatg acacggctca cggcgcagct caatgtgaat aacctgttcg acgtgagcca
    20221 tttcgcgggg agcaatgcct ttgactccgc agtgttcggc atgccgcgct tcttcatggg
    20281 gtcgatccgg atggagttct gaagttgtaa catgtgaaac gtgcggagga gcgcacgttt
    20341 cacgcgcgag taacgcacaa tgccgtccgc accgcgagaa cgcggtgcgg actgtagcaa
    20401 ccatgagcat cgagtggtag acatgtggat cggagtttcg tatggtaaga gtcctgtaac
    20461 tatatggggt tcatcgggtg aacgacatct tccggctcat atccctccgt gggagaacgg
    20521 gacgaactcc gtcgctcctg ctcttacttg cctgtctcct ggttgccatc cggttggcgg
    20581 ccggctttgt gtgtgtgacc tgttttaccg atttcgagaa gccgaccagc cgcagttttc
    20641 atctccacgc cgggggagac ctgaatccgt gccaccatgg ccgggtggag gccggccccc
    20701 tcatcaactg ggcctgcacc gttacgcagg atgacacggc gttcatcctg cccgatgttc
    20761 ctctgctgcc ggtcgtggtc tcgctgtttg ttcccctgct gctcctcgtg gtgtcttatg
    20821 gaaaccgact gctgattgcg gcccacggcc gtagccctcc tcctgtcatc gcctgattcc
    20881 agttacgcaa cgctcctgaa cggtcaccaa tccggctcgg gtcgcatttc atgcggtcct
    20941 gtgccgggtc gtttcactgt cctcaggctc catccccgtg gggtcggcag ggcgtggacc
    21001 cgtggcggcg tcaggaacct cgcggcgcat tccggctcga ttcgttggag tagtaacaat
    21061 ggcgcaagag atcgccgcac cggcgtccct ggcaggaaac caggccggga agacgcaggc
    21121 gcgacctgtc tggaaatatg tgtggcgcac gtggtgggtg caggtgcacc tctacttggg
    21181 attatccgtt ggggccttgc tggtggtgtt cggcgtgacc ggcagtatcc tcgtattttt
    21241 ccaggagatc gatgagtggc tgaatccagt cctgctgacg gtgtctgtgc cgtcgacggg
    21301 gtccgctccc gttcgtccgc tacaggacat tgtgactgcg gctcagcagg cggcgacacc
    21361 ggggagccgg atcgttcagt tgtacggccc ccggaatcgt accggtgtgt ttgcgatcta
    21421 tgccgagcat ccgtcgaaag cctggcaacg aatctatatc gacccttacc gggccgctgt
    21481 gacgggcgtc cgcagttacg ccgcctatga atgggtgccg cactatctga tggacgtcgt
    21541 ctttcagctt cattttgcgc tgtttctggg tgaaaacggg ctgatcatcg tggccctggg
    21601 cgcattgctc ctgctgctgt cgttgatcag cggggtgatt gtctggtggc cgctcaccgg
    21661 ccagtggcgg caggccttcg ttttgagatg gcccagtact ccggtccgtc tgaatttcga
    21721 cctgcacaag accttttcgc tctacacctg tctcgtgctc ggtgcggtct tgctctcggg
    21781 agtctggatg aactggaacg atccgttcgt gtgggtggtc cggctcttgt cccctgcgac
    21841 ccgtgggcct aagcaggctc cggtgtccat tccgctcgaa ggccggcaac cgatcactcc
    21901 cgcgcaggcg ctggcgattg cgacggctcg ttacccggaa ggagtgctga atattctcgt
    21961 gatgccggag gattcgacgg gcatctatct ggtaggccgg agggaggtgc cgggcctcag
    22021 cctcttctgg tctgaacgaa cggtgagtat cgatcagtac agcggcgcca ttctggatgt
    22081 gcgtgagccc gccacccggc gaagcgccgg ggagacgttt gcggactggc aatggccgtt
    22141 acattccggc caggcattcg gattacccgg tcgcctgatg gtctgtctga gcggattggc
    22201 ctgtccggtc atctatgtga ccggcgtgcg gatgtggtgg cgaaagcggt cggcacgccg
    22261 catgcgtccg gcctgaaggg ggtgcgtgcg gagtgacggt tctctgcggt agagtgtcag
    22321 ccgcctgaaa atggaggtct tgtgtctgca caaccgacga tagaacaacc ggaaccgagg
    22381 aagcccagta ttcatgacgc ggacttggac gggttggcca tccggcgaac tgaaccgcat
    22441 ctgccccggc gcaacgggcg gggccggtgg tggctgtggc tcctgctcct gaccctggtg
    22501 acgggcggcg cgtttgtggc ctggaagtca ggtctcaccg gctacggtga gacggttgag
    22561 gtgaccccgg tggtgcgcat gccggcaggc ggcgtctcag ggctcgccgc cacagggtac
    22621 gtcgtcgcgc agcgccaggc gttgatcgct tccaaaggca cgggtcggct ggagtatctc
    22681 ggggtcaagg tcggcgaccc ggtgaaggaa ggccagatca ttgcgcggct cgaacattcg
    22741 gatatcgatg cgctgttgca gcagatggtg gcgaagctca acgt
//
LOCUS       DAORRQ010000064        22767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_138_length_22767_cov_159.638121, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000064 DAORRQ010000000
VERSION     DAORRQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22767
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138_length_22767_cov_159.638121"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1878)
                     /locus_tag="PK224_16450"
     CDS             complement(64..1878)
                     /locus_tag="PK224_16450"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17387.1"
                     /translation="MTRRSPQHDPPAPRRDSVSCRTFPDPAPLLVASGLEPEQVVKIL
                     SPYGITHPAEADTNIQSMAGDPHTRRILATVLPGLLTEIARTADPDQALNHWERLLSG
                     SVNRSSLLQYLQASPKMLGLLCTIFGNSDSLAFALIRDPMLVYWLAEEDVLTTAPTRQ
                     GMVAALRQSLGSLKAIELRLDVLRRVRRREMLRIGVRDLLHLATVEDTTGSLSDLASV
                     LIHAAYEIVDQDLKAVHGIPMHRDANGAWIETEFVVIGMGKLGAHELNYSSDVDLIYV
                     YASADGETRKGKGGRQSNSHGLSNEEYFELLARSLTKALAEQTREGAVFRVDLRLRAE
                     GSVGQLARSVEAYEKYYAQRGQVWERLALLKAWPIAGSPAVGQAFVRMVEGFVFQPDL
                     QPLSRERALAIMRDVRSVKEMIDEKIAGRGHQHRNVKLGTGGIRELEFLVQTIQVLIG
                     KAVPEILDRRTVGALHRFCQHRFVEEDDQRRLTEAYWFLRDVEHKLQMMHDLQTHALP
                     DSDEELQRCAIRMGYSAESREGALAKFLADRQRHTAIVHQTFRSLFYHPETSALFAAV
                     VAAAEGHSRRSASRLEKETGPAGPPPDRSSTAKKKRKL"
     gene            1877..4225
                     /locus_tag="PK224_16455"
     CDS             1877..4225
                     /locus_tag="PK224_16455"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17388.1"
                     /translation="MAPHYTGAILPVQRDHLRVYSTGIMLHANADRDQILTTLAPLCQ
                     GIDRDILQDFVTRMDPDYFSAFPPKTLAAHVKQAATLTPDHPCDVSIAETAGGHFVIT
                     IVAYDYFSEFATICGLLSAFGLNIEEGRIFTSAESEQPSRSRSADSYPIRTRLQGRPG
                     LTRKKIVDVFTVSPIEGQTFAAADRKRLTDQLARMIMLLDEGQLDEARQQVNRQLVEH
                     LGKRRSSFSGLLHTVQITFDNSQSATDTIMDIRSDDTPAFLYAFANALAMRNVYISKA
                     LFAIEDGKLHDRFYIRNRFGQKLLDPGDLEQLRLTAVLIKQFTHALTWAPDPAKALEA
                     FDQFLDLVLEGSRQAGRNQAWAFVKDKNTFPLLARLLGASDFLWEDFLRRQHVNLLPL
                     LKDYRDAPLIKSQATLRKELNRAIVKAKKDEARKEALNRFKDQELFRIDMKHIVEPGT
                     SLPDFSLAISELAEVIVERSLVDCQAKLTKLYGSPRLTNRKPCPFAILGAGKFGGKEM
                     GYASDIEVLFVYGGPGRTSGKQGIENSEYFERLAQEFLQWIEAKQEGIFHIDVRLRPH
                     GGKGSLANAFDEVCKYYSAEGQAAPFERQALIKLRHIAGDAALGKKVETHRDSFVYSG
                     APWDLTVALDLRRQQVKQLVEPGQINLKHSHGGIVTLEYAIQYLQVMHGHRHPSLRTP
                     NTLRALAALIDVGLIPRATGENLRKSYLFIRMLIDGLRMVRGNTKDLVIPPPDSDEFI
                     FLARRVGYQTEDWQAGARHLQTDIEEHMKQNRQFFEKMFGKV"
     gene            4690..5790
                     /locus_tag="PK224_16460"
     CDS             4690..5790
                     /locus_tag="PK224_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB17389.1"
                     /translation="MAYNWEQPDWPNFRYDLSGLQDSLLLLAERTGRASGLLQGLSPD
                     AQVEATVMIMVTEALKTSAIEGELLSRKDVMSSIRKNLGLETGSLSGDKRAQGAAALM
                     LAVRNTIESPLSEDLLFAWHRTVMAGHRHVATGQWRTDAEPMQVVSGAYGHETVHFEA
                     PPSSRVPSEMARFIRWFNETAPSGRKAIQKAAVRSAIAHLYFESIHPFEDGNGRIGRA
                     LSEKALSQGLGRPALLSLSTAIEAKCRDYYDALKEAQQSNEITPWVTWFVRMTVEAQA
                     QAEAQIDFTLKKSRLFDRHRNGLNERQLHILTRMLEEGPSGFEGGMSAKKYMSNTGAS
                     KATATRDLQELTTKGIFEPIGGGRSTGYKINL"
     gene            6023..6736
                     /locus_tag="PK224_16465"
     CDS             6023..6736
                     /locus_tag="PK224_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17390.1"
                     /translation="MSPETILRKEDCEALRTAEELIVWIESVHAQFEATDATRAYARM
                     GKGLVKPFYEEIVPLGDLARHKYLGQPHFCLRPKIGNQNYDAEIINKSSSHEHIKRIE
                     FTSTYRDADFALRLEYLNQHGAVFMSGPVWRDGTKASGGQIHVVPECIDYDNNLNDLT
                     DLIKRRVSIKLGLSYAPNTIIAIVFDDVRHRSTTHLPQLQTYLRDTLSDQIRGKFCGI
                     FILGTSGKTFLEYSETSSV"
     gene            7353..7649
                     /locus_tag="PK224_16470"
     CDS             7353..7649
                     /locus_tag="PK224_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRB17391.1"
                     /translation="MAKTLSLSEVKTRLPELVAGVQEREEEVIVTKNGRPAAILINVD
                     EYTRLKETVDVLSDPGLMSQIAESRAFYKTKHKGLSFEDVFGEPLTPVKKRRTA"
     gene            7646..7939
                     /locus_tag="PK224_16475"
     CDS             7646..7939
                     /locus_tag="PK224_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB17392.1"
                     /translation="MTPFRPDIPPHVAEVIRTLHPDLKQLIKSAIRAIAANPECGEPL
                     QRELDGLRKYRVRRFRIVYAVEQKRRVIRLMAVGHRRYVYEELTDRLRRKTRE"
     gene            8020..9093
                     /locus_tag="PK224_16480"
     CDS             8020..9093
                     /locus_tag="PK224_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HRB17393.1"
                     /translation="MPTLFTPLQVGNIRLPNRIVMSPLTRARAGTTHIPNDMMVDYYS
                     QRASSGLMMTECTMVDAHACAFIGEGGIYSSAHVAGWKRVTDAVHTKRGRIFMQIWHP
                     GRAAHSSLNDGEQPVSSSAKPIRNDVTKTPEGAKPYEVPRPLRTEEMPRYVEMFRLAA
                     RNARQAGFDGVQIHGAHGYLIDNFLRDGVNARTDAYGGSIPNRTRFLLEVTDAAIGVW
                     GAGRVAVRISPLVPFNDMVDSQPEALVTYVAQELSRRAIAFLEIRHENHALPEEQAIL
                     KVARGHFPGVLMSNGSYTRESGESIVAGGAADAIVYGRPYIANPDLVERFAKQAPLNQ
                     VNYDRLYGGGPDGYSDYPALAAG"
     gene            9253..9687
                     /locus_tag="PK224_16485"
     CDS             9253..9687
                     /locus_tag="PK224_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17394.1"
                     /translation="MMLRRLSVVLLCCAGLAACGESGDSTGSVLKPVGEWAKIGETDN
                     YVYYADYASIKKADETVVMLDLFDYKSVQTEGGNAPALSKATQREYDCQNKKSQSFKS
                     SWFSGQMGAGNVVRSAATSNQWSVATQGTATGGLLKAACGNS"
     gene            complement(9780..10556)
                     /locus_tag="PK224_16490"
     CDS             complement(9780..10556)
                     /locus_tag="PK224_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB17395.1"
                     /translation="MAKRVKSSQEPADLLSRHIEQIQATLVAIQPFLSRRKATASLDG
                     FDEQTEEVLSDTFGGASEELEAYQYAKLGEAAILPEEAQEAGTHNVDRESLHQRKQVL
                     ESCLAQLELRKATRAGRDWRRTLGERIDGHTVAEFMSAEVRSVHKSASIKEAGRLLQK
                     WRIGSLLVDDGSRYIGIITDTDLSRKAVAKGLDPNTTTVLSCMSKSVVTIEDSEPLME
                     ALRLMKKEGIRHLPVTEDGTIIGVLSVGDLLRAYQKVLEL"
     gene            complement(10801..12396)
                     /locus_tag="PK224_16495"
     CDS             complement(10801..12396)
                     /locus_tag="PK224_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HRB17396.1"
                     /translation="MNKIEAIKSERDGLTVRDMIAHYAQAGWEAIPEDDIQRLKWYGL
                     FLRNPTPGHFMLRVRLPGGQTTSAQLETLADIAQQYGNGVIDVTTRQQVQLRHLTIAH
                     VPSVFSKLEEAGLTSLQTGMDTVRNIMTCPVAGLNPNELLDGTDIVRAINHEVLGNPA
                     YTNLPRKCNIAVTGCPDNCLHTETQDIALVPAYHDLGHDKCYGYNVLVGGKLGSGGYR
                     IAGSLDMFVNPAEAFEVCRTLLHIYRDHGPRENRTQARLAFLVEAWGEARLRHEVERL
                     VGRTLPTAGVDARGAAERDHIGIFRQKQRGMNYVGLKVLVGRVKAADLRSIAALAVRY
                     GNGEVRLSPAQAFVIPHISDRLVGELAEEPLVKQFAYNPSALYKGLVSCVGSDYCNLA
                     VIETKSRAVETARVLERKLGDTLKPITLHWSGCPAGCGNHLVADIGLLGKKAKVGGKV
                     VDAVDVFVGGRSGPDPKLATKIMEDVPCDRLPAVLEMIMPYHTREKMHRVRGKAVPKG
                     TGASKSTASGNETTGLTPQPLPA"
     gene            complement(12396..12590)
                     /locus_tag="PK224_16500"
     CDS             complement(12396..12590)
                     /locus_tag="PK224_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17397.1"
                     /translation="MSTNAETAVLTVLEHQAPQTFEQLMALSGLSASQVLLVVDQLSR
                     AGAVVLRKSGPDYSVALGGR"
     gene            complement(12642..13082)
                     /gene="cynS"
                     /locus_tag="PK224_16505"
     CDS             complement(12642..13082)
                     /gene="cynS"
                     /locus_tag="PK224_16505"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:TIGR00673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HRB17398.1"
                     /translation="MEKAAVRKAIKAQRLNKKVTIAEVAKTVGKNPTFVAAALNGNHR
                     LSPEEAGKVGKLLDLDKEQISSLSAFPVRADFPNATDPFKYRLLEVIGVYGDSMREMA
                     NEMFGDGIMSAIDFTLDMEKVTGSQGEARCKITLNGKWLEYKTF"
     gene            complement(13136..13966)
                     /locus_tag="PK224_16510"
     CDS             complement(13136..13966)
                     /locus_tag="PK224_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRB17399.1"
                     /translation="MHTADHERIAGVAAKKWSFFERSPGGYLILSALAGIYLGFGIAL
                     IFSVGGPLAAAGSPVVKLVMGVSFGIALTLVIFAGSELFTGNNLIGTIGGLTRSLTWA
                     QVIQLNAWSWLGNLAGSLGLAWLIVQSGVFAKGPTTELIEKVASVKMSLPAWELFVRG
                     ILCNWLICLAVWMTGRTNNDTAKILLIFWCLFAFIGTGFEHSIANQSLLGMALFLPHD
                     AAVSWGAFWYNQLFVVLGNLVGGGLFVGGLYWMVSPYRVTEATAAPAAQPVVSAVAGS
                     "
     gene            complement(14281..15690)
                     /gene="glnA"
                     /locus_tag="PK224_16515"
     CDS             complement(14281..15690)
                     /gene="glnA"
                     /locus_tag="PK224_16515"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRB17400.1"
                     /translation="MNVREVLDFAKKNKVQVVDMKFVDLIGTWQHFTIPVSELTEGLF
                     KDGSGLDGSSIRGWRAINNSDMLLVPDPATACMDPFCSVPTLSLIGNVVDPITREMYD
                     RDPRFIAQKAEKYLQSTKIGDTSYWGPEAEFFIFDHARYDQTNHSAYYHIDSDEGVWN
                     MGQEGVNLGGKIRHKEGYFPVPPTDTQQDIRTEMILEMEKAGIATEKHHHEVATAGQA
                     EIDIRFDSLLRTADKMMMFKYIVKNVARRHGKTVTFMPKPIFGDNGSGMHTHQSIWKD
                     GKPLFAGKEYAGVSQMCLHYIGGILKHAPALAAFTNPSTNSYKRLTPGFEAPVLLAYS
                     SRNRSAGIRIPMYSPSPKAKRIEVRFPDPAANPYLAFAAMLMAGLDGIENKINPGEPA
                     EKDLYDLEAKEAAKIRTMPGSLDEALNHLEKDHQFLLKGGVFSEDLIEAWIGYKRTKE
                     VDTMRLRPHPYEFFLYYDV"
     gene            complement(15741..18425)
                     /gene="glnD"
                     /locus_tag="PK224_16520"
     CDS             complement(15741..18425)
                     /gene="glnD"
                     /locus_tag="PK224_16520"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRB17401.1"
                     /translation="MSHAVNADSHTAQDAAPASVSQLLVEQRQAIRQRLLAGASGDEV
                     VTATTDMVDGLIVGRYRTAARTGGEALTTAGFQHCCLVAIGGYGRRELAPYSDIDLMF
                     LFHPDAAKVMPELVKQVLHPLWDSGFQVGHSVRTIQDCVDLGLADLTVRTSMMEARFL
                     AGSADLFQQFHARYVRKVVAHGFDTYLDQKVTERQREYQKFGETVYLLEPNVKKSKGG
                     LRDLHLLQWIGAARFHTATIRELSDRGILSQADYRAIKEAKEFLLRVRSFLHTRAGMA
                     QEILTFDEQVWLATHLGFTDRPHLLAVEQFMQQYYRHTMGLHAALMRFVERCRRRSLW
                     QRVVRWLPSPRIDQYFVVDGEALTVPVELRSQVLDRPASLLTLFDLARSRRLNIDPSL
                     LEDIHRQAETLSAEQFHTPETGRTFLSILAGPGTARTLEAMHRAHLLEKLVPAFSRVR
                     GLMQFNQYHKYTVDEHSLLAVAKAEALAEHSGVLGEVYREIKRKDLLHLALLLHDLGK
                     GHEEDHSEVGKRLAEETAARLGFDERDSRTLVMLVHRHLLMAHTAFRRDPYDEKVLLP
                     FAREVGTPEVLRKLLALTAADIAAVGPDVLTKWKESLLIELYLRAMQEVSGERETADE
                     PQRLARIADDVASQQGADRDLLSDKAWIRSELEQFPLRYVYGTSPRRIAAHLGAIRRL
                     QVGGVVVETEFNAPLGTCEYAVIAHDDLIPGLFSKIAGVMAAGGLQILDAQIVTRKDG
                     VVVDTFQVADPDYQGAPPADRCESIAGTITEVLTGRQAIETLMRRGTRLNMGRSLPAH
                     RQPSEVRIDNETSDRFTILDVFADDRQGLLYIITNAIFQLGLSVHASRISTRLDQVAD
                     VFYVTGMDGKKVEEAGRLESIRASILNEIELFLGAHAA"
     gene            complement(18428..18766)
                     /locus_tag="PK224_16525"
     CDS             complement(18428..18766)
                     /locus_tag="PK224_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRB17402.1"
                     /translation="MKLIEAIVKPFKLDEVKDALLEIGIQGMTVTEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVNDSQVQRVLETITRAAKTGSIGDGKIFVRELTSAVRI
                     RTGETGESAL"
     gene            complement(18874..20205)
                     /locus_tag="PK224_16530"
     CDS             complement(18874..20205)
                     /locus_tag="PK224_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRB17403.1"
                     /translation="MVKRSAQRKTVVVGVAGVLILLAESWGTQVWAQNTPLKVDTGDT
                     AWVLVSSAFVLAMLMPGLALFYGGLVRTKNVLGTIMQSVMILSVVSLLWILFGYSLAF
                     GPDKGGVIGGLEWVGLSGVGSEPHPVYGPTIPHQAFMLFQLMFAAIAPALITGAFAER
                     KRFTSVILFAALWSVFVYVPLAHWIWGGGWLAKLGALDFAGGAVIHISSGAAALVCAI
                     VLGKRRGYGTDYMAPHNLPMTLLGTGFLWFGWFGFNGGSALGANGIAVSAIIATHAAA
                     AMGAIAWCGAEWAHRGKPTVLGVASGAVAGLATVTPAAGYIAPMSAIAIGLVAGMTCY
                     AAIVWKGRFGYDDSLDVVGIHGVGGVIGILATGLFASKVVNPGGADGLFFGNPGLFGI
                     QLLVAAVTTIFSIIGTFVILKLVDSMTGLRVSSEEEATGLDLSQHNERAYS"
     gene            complement(20280..21158)
                     /locus_tag="PK224_16535"
     CDS             complement(20280..21158)
                     /locus_tag="PK224_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17404.1"
                     /translation="MNMLVGRKARTWVLGLALLAGGGGGALGEAQAGTTDDLMDTTKH
                     PVSVTMAMQADSFFGFNPSIYGTYGLTDNIALAFNFTYWTMINGVGVHDNAPWLETDM
                     GLNFTFLEKRLSVTPMIGFVHGQLLSSRGGFFPNGGGSVNERTTAFEGVVPNIIANYT
                     DDRFEGEFYLGYYKAIRSEGGSGGGGATGCTGLVDTNCLGTSQTGGRGTWDFLHYWVS
                     AGMRVNSMWSLGAHYEHLLTTRDSSAPGAAQDYYRWIGPYVEMKLPGNMAFRFTVGKD
                     LTDNQDFYKLKFTKTF"
     gene            complement(21295..>22767)
                     /locus_tag="PK224_16540"
     CDS             complement(21295..>22767)
                     /locus_tag="PK224_16540"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRB17405.1"
                     /translation="SFPLSGRHELYNEAAVLSDRRILATYRKRHLPNYGVFDESRYFH
                     PGSRLPLLVLNGATIGVNICEDIWFSDGPTRAQAAAGAEVIVNINASPFHVGKGRMRE
                     QVLATRARENRVIVTYTNTVGGQDELVFDGCSMIVDQAGEIAGRGKAFDQDLIVADLD
                     VAAVGRARLAQGRKKPLPPRVAALIDRVDVRLPARKSQSVVVPDLEPPLGRLDEAYRA
                     LVLGVQDYVRKNGFKRVVIGLSGGVDSAITAVIAVDALGAENVLGVFMPSPYTSRASR
                     EDVADLASRLHIQVDTLSITATFKSYLRALSRSFKGRRPDTTEENLQARIRGNLLMAY
                     SNKFGHLVLTTGNKSEMSVGYATLYGDMAGGFAVIKDVPKTMVYELSYMRNQVGPAPV
                     IPKRVLERAPTAELRPDQKDEDSLPPYAILDPILKAYVEEDRALEDIAAMGFDRETVA
                     RVIALVDRSEYKRRQAPLGIKITHRAFGKDRRMPITNGYR"
BASE COUNT         4838 a         7304 c         6128 g         4497 t
ORIGIN      
        1 ggaagagaaa aaagaagctg ccggtggcca tgctggtcac aaccacggca tggaaggtat
       61 gtactaaagt ttccgtttct tcttcgctgt cgaagaccgg tccggcggtg gccccgccgg
      121 gccggtctct ttttcgagcc gagacgcgct gcggcgggag tggccttccg ccgcagccac
      181 cacggcggca aacagggccg aagtctccgg gtggtagaag agcgaccgga acgtctgatg
      241 tacgattgcc gtgtgccgct gccggtccgc tagaaacttg gccaacgccc cctcgcgcga
      301 ctccgcgcta tatcccatcc gaatcgcaca acgttgcagt tcctcatcgc tgtcaggcaa
      361 cgcgtgcgtc tgcaaatcat gcatcatctg caacttatgt tcgacatccc gtaagaacca
      421 ataggcctcg gtcagtcgac gctgatcgtc ttcctccacg aaccgatgtt ggcaaaaccg
      481 gtgcagggcc cccactgttc tacgatccag aatctcagga accgccttgc cgatgagcac
      541 ttgaattgtt tggacaagga attccagttc tcgaatcccc cccgtgccca atttcacatt
      601 ccgatgctga tgtccgcgcc ccgcgatctt ttcatcgatc atctctttga cggaacggac
      661 atcgcgcata attgccagcg cccgctccct cgacagaggc tggagatcag gctgaaaaac
      721 aaatccttcg accatccgca cgaacgcctg tccgaccgca ggagaaccgg cgataggcca
      781 ggccttcagc aacgccagcc gctcccacac ctggccacgc tgggcataat atttctcgta
      841 agcttcgacc gatcgtgcga gctggccgac cgaaccctct gcgcgcagcc gcaaatcgac
      901 gcgaaacacc gcaccttcac gagtctgctc ggccagggcc ttcgtcagtg aacgcgccag
      961 cagctcaaaa tattcttcat tggacagacc atgactgttc gattggcgcc cgccctttcc
     1021 cttcctggtc tcaccatctg cggaggcata aacataaatc agatccacgt ccgagctgta
     1081 attcagctca tgcgcgccga gtttgcccat cccgatgacg acaaactccg tctcgatcca
     1141 ggcgccgttt gcgtctcgat gcatcgggat cccatggacc gccttgagat cctgatcgac
     1201 aatttcataa gccgcatgga tgaggaccga ggcgagatcg gacaaggaac cggtcgtgtc
     1261 ttccaccgtg gccaggtgca gcaggtcccg cacaccgatg cgcagcattt cccggcgacg
     1321 cacccgccgc aggacatcca gccttaattc gatcgccttc agcgagccca ggctttgacg
     1381 aagggccgct accatccctt gccgggtcgg agcggtcgtc aagacatctt cttctgccag
     1441 ccaatacacg agcatggggt cgcgaatgag ggcgaaggcg agagaatcgc tgtttccgaa
     1501 aatcgtacag agcagtccca acattttcgg cgaggcttgc agatactgca ggagcgagga
     1561 gcgattgaca ctccccgaaa gcagtcgctc ccaatgattc agggcttgat caggatcggc
     1621 cgtgcgggca atctccgtca agagaccggg caacaccgta gccagaatcc ggcgtgtatg
     1681 cggatccccg gccatactct ggatattcgt atccgcttcg gccggatgtg tgattccgta
     1741 ggggctcaga atcttgacca cctgctccgg ctcaaggccg gacgccacca gcaaaggagc
     1801 tggatcggga aaggtccggc aggaaacgga atcccggcgc ggggcaggcg ggtcgtgctg
     1861 tggcgaacgt ctggtcatgg cgccgcatta tactggcgca attcttccgg tacaacgcga
     1921 ccaccttcgg gtatactcca ccggaatcat gctgcacgca aacgcagacc gcgaccagat
     1981 cctcacaacc ctggctccac tctgtcaggg catcgaccgc gacatcctgc aagacttcgt
     2041 cacgaggatg gacccggact atttctccgc ctttccgccg aagacactcg ccgcacacgt
     2101 caagcaagcc gccacgctca caccggacca cccctgtgac gtgtcgattg cagaaaccgc
     2161 aggaggccac ttcgtcatca ccatcgtggc ctacgattac ttttccgaat tcgccacgat
     2221 ctgcggcctc ctatctgcct tcggtctcaa catcgaggaa ggtcgcatct tcacctcagc
     2281 agaaagtgaa caacccagcc gtagccgatc agccgattcg tacccgatca ggacaaggct
     2341 gcaaggtcgc cccgggctta ctcggaagaa gatcgtggac gtgttcacgg tgagccccat
     2401 cgaggggcag acgttcgccg ccgcagaccg gaaacgcctg accgaccaac tcgcccgcat
     2461 gatcatgctg ctcgacgaag ggcagctgga cgaggcccgg caacaggtca accgccaatt
     2521 ggtcgaacac ctgggcaaac gccggagttc cttcagcggc ctgctccaca cggtccagat
     2581 caccttcgac aacagccagt cggcgaccga tacgatcatg gacatccggt ccgacgacac
     2641 gccggcgttt ctctacgcgt tcgcgaacgc actcgcaatg cgcaacgttt acatcagcaa
     2701 agcgctgttc gcgatcgaag acggcaagct gcacgaccgt ttttacatcc gcaaccgctt
     2761 cggacagaag ttgctcgacc cgggcgacct ggagcagctg cgcctcaccg ccgtgctcat
     2821 caaacagttc acccatgccc tcacctgggc gcccgatccg gccaaggcgc tggaagcctt
     2881 cgaccagttc ctggacctcg tactcgaagg ctcgcgtcag gccggacgaa accaggcctg
     2941 ggccttcgtg aaagacaaga acacgtttcc gctcctggca aggctgctcg gggcgagtga
     3001 ttttctgtgg gaagacttcc tccggcggca acacgtcaac ctgctgccgt tgctgaaaga
     3061 ttatcgcgat gcgccgctca tcaaatccca ggcgacgcta cgcaaggaat tgaaccgcgc
     3121 catcgtcaag gcgaagaaag acgaggcacg gaaggaagcc ctcaaccgct tcaaggatca
     3181 ggaactcttc cgcatcgata tgaaacatat cgtcgaaccg gggacgagcc tgcccgactt
     3241 ctcgctcgcc atctcagaac tggctgaagt gatcgtcgaa cgcagcctcg tggactgcca
     3301 ggccaaactg acgaagctct atggcagccc ccgtttgacc aacagaaagc cctgtccctt
     3361 tgcgatcctg ggcgcgggga aattcggcgg caaggaaatg ggctacgcct ccgacatcga
     3421 ggtgctcttc gtctacggcg ggcccggacg cacaagcggc aaacagggga tcgagaacag
     3481 cgaatacttc gaacgcctcg cgcaggaatt tttgcaatgg atcgaagcca agcaggaagg
     3541 catttttcac atcgacgtcc gactgcggcc ccacggcggc aaggggtccc tcgccaacgc
     3601 gttcgatgag gtctgcaagt attacagcgc agaaggccag gctgctccgt tcgagcggca
     3661 ggcgctgatc aagctgcgcc atatcgcggg agacgccgcg ctcggcaaga aggtcgagac
     3721 tcatcgcgac agcttcgttt acagcggcgc gccctgggat ctgacggtgg cgctcgacct
     3781 gcggcggcaa caggtcaaac aactcgtcga gccgggccag atcaacctca agcacagcca
     3841 cggcggcatc gtcacgctgg aatacgctat ccagtacctg caggtcatgc acggccaccg
     3901 gcacccgagc ctgcgcacgc cgaacaccct ccgagcctta gccgccctga tcgacgtagg
     3961 gctcattccc cgcgcgaccg gcgagaacct gcgcaagtcc tatctcttca tccgcatgct
     4021 gatcgacggc ctgcgcatgg tgcgcggcaa caccaaggac ctcgtcatcc cgccgcccga
     4081 ctccgacgaa ttcatcttcc tcgcccgccg cgtcggctac cagaccgaag attggcaggc
     4141 cggggcaaga catctccaga cagatatcga agagcacatg aagcaaaaca gacagttctt
     4201 cgagaagatg tttgggaagg tgtgaaggaa ggtaagaaga tggaggagga caggtcttct
     4261 cggttccggc gatggctcaa gcatgtaggg ccttgtggtc cctgggctgt gcaactcgtt
     4321 ctattccgga ttctatccga atggaacttc gaacagtgct caccctcgcc tccggcgacc
     4381 gcgcccccaa ggccctgcga gccatcgcaa gtcgccgctc tcacctgtcc tcctccaact
     4441 cagagagagt gggcagcagc aagcccagca ggaccattga tatcaactcg caatcgggga
     4501 gcagtcaagc ccgctcccat gaagggaaac cggcaagatt agaagcaata tgtagatttg
     4561 gagccctcta ccggtcgctg ttctcactcc acaccctaaa accagccaaa ggatagatga
     4621 gccgatcaga gaacgtattc ggctcatatt tgacttaata catgagccga atatctatat
     4681 aatcggctca tggcctataa ctgggaacag cctgactggc ccaacttccg ctacgacctc
     4741 tccggtctcc aagacagcct ccttctactc gccgaaagaa ccggccgtgc gagtgggctc
     4801 ctgcaggggc tgagccctga cgcgcaggtc gaagccaccg tcatgatcat ggtgaccgaa
     4861 gcgctcaaga cctcggcgat tgaaggcgaa ctcctcagcc gcaaggacgt catgtcttcg
     4921 atccgaaaga accttgggtt ggagacaggc tccctcagcg gagacaagcg cgcacaaggc
     4981 gccgccgcgc tgatgctggc cgtccggaat acaatcgaga gccccctgtc ggaagacctg
     5041 ctctttgcct ggcatcgcac agtgatggca ggacaccggc atgtggccac gggacaatgg
     5101 cggaccgatg cggagccaat gcaagtcgtg tccggtgcat acgggcatga aacagtccat
     5161 ttcgaggccc ctccttcctc tcgcgttcca agcgagatgg cgcggtttat ccgctggttc
     5221 aacgagacgg ctcccagtgg gagaaaagcc attcagaaag ccgcggtgcg ctcagccatt
     5281 gcacatctgt acttcgaatc gatccatcct ttcgaggacg gcaatggccg catcgggaga
     5341 gccctttccg agaaggccct ttcgcaggga ctcggccgcc ccgccttgct gagcctttca
     5401 accgcgatcg aagccaaatg tcgcgactat tatgacgcgt tgaaagaggc ccaacagtcc
     5461 aatgagatca ctccctgggt gacctggttc gttcgcatga cggttgaggc gcaggcccag
     5521 gcagaagcgc aaattgactt cacgctgaag aaatcgcggc tgttcgaccg gcatcgcaac
     5581 ggactcaacg agcggcagtt gcacatcctg acccgcatgc tcgaagaagg tccaagcggc
     5641 ttcgagggcg gaatgagcgc caagaaatat atgtcgaaca ccggcgcctc aaaggccacg
     5701 gcaacacgcg acctccagga actgaccacg aagggaatct tcgaaccgat cggtggtggg
     5761 cggagcacag ggtataagat caatctgtaa aatggacccc gcggtggtag caaaagccgg
     5821 cacacagctt cgtcagctac cagacggagg attaacgagt aaaggcgagc cctctgacgg
     5881 atggcgtggc gaacatgacc gcgactaaga atgtcttcga aaggacgttt tgggcaggcg
     5941 tacaccttat ttgctcaatg tttgattgca aggcctgaca ctatctttcg cataggcaat
     6001 cttgccctgg aggaattgcg acatgagtcc cgagaccatt cttcgaaaag aggattgcga
     6061 agccctgcgc accgcggaag aactgattgt ttggatcgaa tctgtgcatg cccagttcga
     6121 agctactgac gcaacaagag cctatgctcg gatgggcaag ggcctcgtta aaccatttta
     6181 cgaagagatc gtgcccttgg gagatctagc gcgacataaa tatcttgggc agccacactt
     6241 ctgtcttcgc ccaaagatcg gcaaccaaaa ctacgatgcc gagatcatca acaagtcatc
     6301 cagtcacgaa cacattaagc gaatcgaatt cacgagtacg tacagggatg ctgacttcgc
     6361 cttacgcttg gagtacctga accagcacgg ggcagtattc atgtcggggc cagtctggcg
     6421 cgacggaacg aaagcctcag gaggccaaat ccacgtcgtt cctgagtgca tagactacga
     6481 taacaattta aacgatctca cagaccttat aaagcgacgg gtgtccatca aattaggctt
     6541 gtcctacgca cccaatacta ttatcgctat tgtctttgat gacgttcgcc atcgttctac
     6601 aacgcaccta cctcagctcc aaacatacct tcgagacacc ctaagcgatc agatacgcgg
     6661 caagttttgc ggcatcttta ttctagggac gtcaggtaag acatttctgg aatatagcga
     6721 aacatcttcc gtttagatcg actaaaagag tccgggtctt tgctgattgc tcagagggat
     6781 ggaaacggat cggcccagct tcgcggtgca cctgtcgaaa tacgcgaatt gacaatctca
     6841 tctagatggt gaagaaaacg gtgtcaggaa tcatttttcg gtgcgtaacg gctcaccctg
     6901 gaatacgtga agtcccaacc tctcccgttg acactttctt gctgggagag tagccctaga
     6961 acctatctca gaattcaaaa ccccgctcga cggggcttca accactcaag gctcaatgcc
     7021 ggggcttcga tattcttaag aattctgctg tttactcttg gtcatggcca ggcacccgca
     7081 gccactacga ctcacaaatt gattttgaga taggttttgt tttggccagc aatgggttgt
     7141 gtatcttgcc tggctactcg gcgtgaggaa ctcactacaa cggggtctct tggcattcga
     7201 cagccagcgt ctttcgcctg agttcgtacc tctgacattc cccctccccg ctcacgttgc
     7261 acagcgacca cttgcccgct catttgacgg agcctcacct gtcgctgtat tctaacgacc
     7321 agaattctgg tcttttatac atgaggtctg ccatggcaaa aacattgtcg ttgtcggagg
     7381 tcaaaacgcg cctgcctgaa ttggtggctg gggtacaaga acgcgaggag gaagtcattg
     7441 tcacgaagaa cggccggccg gctgccattt tgatcaatgt cgatgaatat acccgcctca
     7501 aagagaccgt ggatgtcctg agcgatcctg gactcatgag ccagatcgcc gagagccggg
     7561 ccttttacaa aaccaaacac aaagggctct cgtttgaaga cgtgttcggc gaacccctca
     7621 cgcccgtcaa gaagcgtcgc acggcgtgac gccattcaga ccggacatcc ctccgcatgt
     7681 cgcagaggtg atccgtacac tccatcccga tctgaagcaa ctgatcaagt cagccattcg
     7741 tgccattgcc gcgaatcccg aatgcgggga gccgctccaa cgggaactgg acggattacg
     7801 aaaatatcgc gttcgccgct ttcgcatcgt ctatgccgtt gagcagaaaa gacgagtcat
     7861 ccgcctgatg gccgtcggcc accgtcgcta tgtgtatgaa gagctgaccg accggcttcg
     7921 acgcaagacc cgtgaatagc actacgccgg ttgactccgc cgtcatctcc ctcataagtt
     7981 ctttcaccga acatccaccc agtgctcaaa ggaacaccta tgcctacatt atttaccccg
     8041 cttcaagttg gaaatatccg attacccaac cgcattgtca tgtcacccct tacccgcgcg
     8101 cgggccggaa ccacacacat tcccaacgac atgatggtgg attactactc ccaacgcgcc
     8161 tcgagcgggc tcatgatgac ggaatgcacc atggtcgatg cccatgcctg cgcctttatc
     8221 ggtgaaggcg gcatttacag ctccgcgcat gtggcaggct ggaaacgagt gactgatgcc
     8281 gtgcacacca agcggggccg catctttatg cagatctggc accccggccg tgccgcacac
     8341 tcctcgctga acgacggcga acaaccggtc tccagcagtg ccaagcctat ccgaaatgac
     8401 gtaaccaaga cgccggaggg ggcgaaaccc tatgaagtgc cccgcccgct tcgcaccgaa
     8461 gaaatgccgc ggtatgtgga aatgttccgg ctcgccgcac ggaacgccag acaggccggc
     8521 ttcgacgggg tacaaatcca tggcgcccac ggctatttga tcgataactt tctgcgcgac
     8581 ggcgtcaatg cacgcacgga tgcctatggc ggttctatcc ctaatcgtac tcggtttctg
     8641 ctggaagtca ccgatgccgc catcggcgtc tggggagccg gacgtgtggc ggtgcggatc
     8701 tcgcccctgg tcccgtttaa cgacatggtc gacagccaac ccgaggcgct ggtgacctat
     8761 gtcgcgcagg agttgagccg gagagcgatt gcgttcctgg aaatccggca cgagaaccat
     8821 gccttgcctg aggagcaagc tattctgaag gtcgcccgcg gacatttccc gggtgtgctg
     8881 atgagcaacg ggagctacac acgcgaaagc ggtgaatcga tagtggcagg cggagcagcc
     8941 gatgcaatcg tctacggacg tccctatatc gccaatccgg acctcgtcga gcgcttcgcg
     9001 aagcaggctc cgctcaacca ggtcaattac gatcggcttt atggcggggg gccggacggc
     9061 tacagcgact atcccgcctt ggccgcaggc tgatcccaaa cagaaccgat cactcgatcg
     9121 gatgacgttc agacccacct ctccggaagc gggaaccaca ctgggttctc tttatgtcta
     9181 cgtagcgcat tgccccgcgt cgagatttgg caaaaccatg ttacagtggg aactgcggag
     9241 tgattggagg ggatgatgct acgacgacta tctgtggtcc tgctttgctg cgcgggtctg
     9301 gctgcatgcg gagaaagcgg tgacagcacc ggctccgtcc tcaagccggt gggagaatgg
     9361 gcgaagatcg gcgagacgga caactacgtg tattacgccg actacgccag catcaagaag
     9421 gccgatgaga ccgtcgtgat gctggacctg ttcgattaca agagcgtaca gaccgaaggt
     9481 ggaaacgccc ccgctctttc caaagccaca caacgcgaat acgactgcca aaacaagaaa
     9541 agccagtcgt tcaagtcgag ctggttttca ggacaaatgg gagccggaaa cgtcgtgcgc
     9601 tccgccgcca cgtcgaacca atggtccgtg gccacgcagg gcaccgccac aggcgggctc
     9661 ttgaaggccg cctgcggcaa ttcctagaat cgcggtcgat tcatgaaaac cggactggcc
     9721 gcatttccgg aggagcttct ttacccgaca agtctactct ggtctccgga ttgccttccc
     9781 tacagctcca gcaccttttg ataggcccgg agcaagtcac ccacggagag cactccgatg
     9841 atcgtgccgt cctccgtgac cggcagatgt cggatgccct ctttcttcat caatctgagc
     9901 gcttccatca gcggctcact gtcctcgatg gtgaccacgg atttgctcat gcaggagagc
     9961 accgtggtcg tgttcgggtc cagtccctta gcgacggcct tccggctgag atccgtatcc
    10021 gtgatgatgc cgatgtagcg cgatccgtcg tccaccagca gggaaccgat ccgccacttc
    10081 tgcagcaaac ggccggcctc cttgatcgac gcactcttat ggacgctcct gacttcggcc
    10141 gacatgaact cggccaccgt gtgaccgtcg atccgctcac cgagggtcct gcgccaatcc
    10201 cgaccggctc tggtggcttt tctcaactca agctgggcca gacaactttc caacacctgc
    10261 ttccgttgat gaaggctttc gcgatccacg ttgtgcgtgc cggcctcctg cgcttcctcc
    10321 ggcaggatcg cggcctcgcc cagcttggcg tactgatagg cttcgagttc ctcggacgct
    10381 ccgccgaagg tgtcactcag cacctcttcc gtctgctcat cgaatccgtc cagcgaggcg
    10441 gtagccttac gtcgggataa gaatggctgg atcgccacca acgtcgcttg aatctgctct
    10501 atgtgccggc tgagaagatc cgccggttcc tgactcgatt tcactctctt ggccatgctg
    10561 cctccttgtt gagggcaaga gaataacgca actatggcat cggctcaagc cttcccggca
    10621 agatgctgaa aatgtccgcc cgctttgttc tcgcgtcgct caaagcctca acgtacctca
    10681 aagggtacgc ctcgccttct cgcatcgctg cggccgcgcg ggacagacat tttgagcatc
    10741 ctgctgggag cactccctac tgccatctcc gtgcttacgt tctgcagctt ccaaaactgt
    10801 ctaggcagga agcggttggg gtgtgagccc cgtcgtctcg ttgccggatg ccgtcgactt
    10861 tgaggctccc gtgccttttg gcacggcctt cccgcgcaca cgatgcattt tttcgcgcgt
    10921 gtggtagggc atgatcattt ccaacaccgc tggaagccga tcgcagggca catcctccat
    10981 gattttggtg gcgagcttgg gatcggggcc cgagcgcccg cccacgaaca catccacggc
    11041 atcgacgacc ttgccgccca ccttcgcctt cttgcccaag agtccgatat ccgccacgag
    11101 atggtttcca cacccggccg gacaaccgga ccaatgcaag gtgatcggct tgagggtatc
    11161 gcccagcttc cgctccagca cccgcgccgt ttcgacggcc cggcttttgg tttcaatgac
    11221 ggccagattg cagtagtcgc ttcccacaca actgaccagt cccttgtaga gggcggacgg
    11281 gttgtaggcg aattgcttga ccagcggctc ttccgccagt tcacccacca gccggtcgct
    11341 gatatgaggg atcacgaagg cttgcgccgg cgagagacgt acttctccgt tgccgtaccg
    11401 aaccgccagc gcggcgatgc tccggagatc cgccgccttc acccgaccga ccagcacctt
    11461 cagcccgaca tagttcatgc cccgttgttt ttgacggaag atgccgatgt ggtcgcgctc
    11521 cgccgctccc ctcgcatcca cccccgccgt gggcagggtt ctcccgacca gccgttcgac
    11581 ttcgtgccgc aaccgagctt cgccccaggc ctccacgaga aatgcgaggc gcgcttgggt
    11641 gcgattctca cgcggtccat gatcgcgata gatgtgcagc agcgtccggc aaacctcgaa
    11701 ggcttccgcc ggattcacga acatgtccag ggagccggca atgcgatagc cgccggaccc
    11761 caacttgccc ccgaccagca cgttgtaccc gtaacacttg tcatgcccca ggtcgtgata
    11821 ggccgggacc aacgcgatgt cttgcgtctc cgtgtggagg caattgtccg gacaaccggt
    11881 gaccgcaata ttgcacttgc gcggcagatt cgtataggcc ggattaccca gcacttcgtg
    11941 attgatcgcg cgcacgatgt ccgtcccgtc gagcaactcg ttgggattca gacctgccac
    12001 ggggcaggtc atgatgttcc gtaccgtgtc cattccggtt tgcagtgacg tcaggcccgc
    12061 ctcctccaac ttactaaaca ccgacggcac atgggcaatc gtcagatgcc ggagttggac
    12121 ttgttgcctg gtggtaacat cgatcacccc gttgccgtat tgctgcgcga tgtcggccaa
    12181 cgtctcgagt tgagccgacg tggtttggcc acccggcagg cgtacccgca acatgaagtg
    12241 gcccggagtc ggatttcgca aaaataaccc gtaccacttc aagcgttgaa tatcgtcttc
    12301 cggaatagcc tcccatcccg cttgcgcata gtgtgcgatc atgtcccgca ccgtcagccc
    12361 gtcccgctcc gacttgattg cctcgatctt attcatcacc tgcctcccaa tgcgacggaa
    12421 tagtccggtc cggatttcct caacaccacc gctcctgccc gactcaactg atccacgacc
    12481 agcaacacct gtgacgcgct caaaccggag agggccatca gttgctcgaa tgtctgggga
    12541 gcctgatgct ccaggaccgt cagtacggca gtttctgcat tcgtgctcat gatgtcctcc
    12601 tttcggttga tcgccatcca cgcatcaggg accggtttct cttaaaacgt tttgtactca
    12661 agccacttgc cgttgagcgt gatcttgcac cgggcctcgc cctgactgcc ggtgaccttt
    12721 tccatatcca acgtaaagtc gatggcgctc atgatcccgt cgccgaacat ttcgttcgcc
    12781 atctcccgca tcgaatcgcc gtagacaccg atgacttcca acaggcgata cttaaacgga
    12841 tccgtcgcat tcgggaaatc ggcccggacg ggaaacgcgc tcagggaact gatttgttct
    12901 ttatccaaat ccagcagctt ccccactttg cccgcttcct ccggtgacaa gcggtgattc
    12961 ccgttcaacg ctgcggcgac gaacgtcgga ttcttcccga ccgtcttcgc cacctctgca
    13021 atcgtgacct tcttgttcaa ccgttgtgcc ttgatcgcct tgcgtactgc tgctttttcc
    13081 attgtgatct ccctttagtg attcgcccgt gaataccgtc ccgcaccgcg cttcattacg
    13141 atcctgccac cgccgacacc accggttgag ctgccggggc cgccgtcgct tccgtcacgc
    13201 gataggggct caccatccaa tagagccccc cgacaaacaa cccgccgccc accaggttcc
    13261 ccagcacgac gaacagctgg ttgtaccaga aggcccccca actcaccgcc gcgtcatgcg
    13321 gcagaaacaa cgccataccc aacaacgatt gattggcgat gctatgctcg aacccggtgc
    13381 cgatgaaggc aaacaggcac cagaagatca ggagaatctt cgccgtatcg ttgttcgttc
    13441 gccccgtcat ccagaccgcc agacagatca gccaattgca gagaatgccc cgcacgaaca
    13501 actcccaggc aggcagcgac attttgaccg aggccacttt ctcgatcagt tccgtcgtcg
    13561 gccctttggc gaacacgccc gattggacga tgagccaggc caaccccaac gagcccgcca
    13621 gattgccgag ccaggaccag gcattgagtt gaatgacttg cgcccaggtg agacttctcg
    13681 tcaggccacc gatggtgccg atgaggttat tgccggtgaa caactcagac ccggcaaaga
    13741 tcacgagcgt caatgcaatc ccgaacgaca cccccatcac gagtttgacc accggagacc
    13801 cggccgcggc caacggtccc cccacactga agatcaacgc aattccaaac ccgagataga
    13861 tcccggccaa ggccgagagg atgagatatc cgcccggtga ccgttcgaaa aacgaccact
    13921 tctttgctgc cacccctgcg atccgctcgt gatccgctgt atgcatgagg ctctcctttc
    13981 ggtggtcact gtgtccgcac aactcaaaaa aaaacgtcct catcccgcat tcgggaagag
    14041 gacgtcgttg tcctgcttgg caaggtcgct acgacctcgc ccggtgctgt gtgccgactt
    14101 cagcgtcgac agcaataaaa aaggcgcccg ccactcttgg aagaatgacg gacgcctttg
    14161 tccgtttacc gtatttcatg cgggtgtgat ttcaggcgtc gttgcctgtt ctcacccaac
    14221 ccctgcatgt ataccatcgc ctcgtggatt cgtcaagcgc tctcgacgag gaaaccctca
    14281 ctacacgtca taatacagga agaactcgta cggatgcggt cgcaaccgca tagtatccac
    14341 ttccttggtg cgcttgtacc cgatccaggc ctcaatgagg tcctcgctga acaccccgcc
    14401 cttgagcagg aattgatggt ccttctccag gtgattcagg gcctcgtcca gactccccgg
    14461 catcgtgcga atcttcgccg cttccttggc ttcgagatcg tacagatcct tctcggccgg
    14521 ctctcccgga ttgatcttgt tttcgatacc gtccaatccg gccatcaaca tggcggcaaa
    14581 cgcgagatag gggttggctg cggggtccgg aaagcgcact tcaatccgct tcgccttcgg
    14641 gctcggggaa tacatgggaa tgcgaatacc ggccgaccgg ttccggctcg aataggccag
    14701 caacaccggc gcttcaaaac ccggcgtcag gcgcttgtat gaattggtcg acgggttggt
    14761 gaaggctgcc agggccggtg catgcttcag gataccgccg atgtagtgca ggcacatctg
    14821 agagacgccg gcatattcct tccctgcgaa cagaggcttt ccgtctttcc agatgctctg
    14881 gtgggtgtgc atgcccgatc cgttgtcgcc gaaaatcggc ttcggcatga aggtgaccgt
    14941 cttgccgtgc cgacgcgcca cgttcttgac gatgtacttg aacatcatca tcttgtccgc
    15001 ggttctcagc aggctgtcga agcggatatc gatttcagcc tggcctgccg tggccacttc
    15061 gtggtgatgc ttctcggtgg cgatgcccgc cttctccatc tccagaatca tttccgtgcg
    15121 gatgtcctgc tgcgtatcgg tcggcggaac cgggaagtac ccttccttgt ggcggatctt
    15181 gccgcccagg ttgacaccct cctgccccat gttccatacg ccctcgtcgg aatcgatgtg
    15241 gtagtaggcg ctgtggttgg tctgatcgta ccgcgcatga tcgaaaatga aaaactcggc
    15301 ttccggtccc caataggagg tgtcgccgat cttagtgctc tggagatatt tctccgcctt
    15361 ctgggcgatg aaccgcggat cgcgatcgta catttcacgc gtgatcggat cgacgacatt
    15421 gccgatgagg ctcagggtgg gcaccgaaca aaacggatcc atgcaggcgg ttgccgggtc
    15481 cggcacgagc agcatgtcgc tgttgttgat cgccctccag cctcgaatcg acgatccgtc
    15541 gagtccggac ccgtccttga acaggccttc cgtcagttcg ctgactggaa tcgtaaaatg
    15601 ctgccaggtg ccgatcaggt ccacgaactt catgtccacg acctgaacct tattcttctt
    15661 tgcaaaatct aacacctcac gaacgttcat cccctcctcc tttcaaccgc cgataaaata
    15721 accgcaccac acgctgtcgt ttacgccgca tgagccccga gaaacagttc tatctcattg
    15781 agaatcgacg cccgaatcga ttccagccga ccggcctcct ccaccttctt cccgtccata
    15841 cccgtcacat aaaacacgtc ggccacctga tcgagccgag tggagatccg ggacgcatgc
    15901 accgacaacc ccaactgaaa aatcgcgttc gtgataatgt acaacaatcc ctgccgatca
    15961 tccgcaaaaa catccagaat ggtaaagcgg tcggacgtct cattgtcgat ccgcacctca
    16021 gacggctgcc ggtgggcagg caacgatcgc cccatgttca gcctggtccc gcgacgcatc
    16081 agcgtctcaa tggcctgacg ccccgtcaac acctccgtga tcgtgccggc aatcgattca
    16141 caccggtcgg caggcggagc cccctggtag tccggatcag ccacctggaa ggtatcgacg
    16201 accacgccgt cctttcgcgt cacgatctgg gcatccagaa tctgcaatcc gccggccgcc
    16261 atcaccccgg cgatcttcga gaacaacccg gggatcagat cgtcgtgcgc aatcacggca
    16321 tattcgcagg ttcccaatgg ggcattgaat tccgtctcca ccaccacccc accgacttgc
    16381 aaccggcgaa tggcgccaag atgcgcggcg atgcgtcggg gagaggttcc atagacgtat
    16441 cgcaggggaa actgttccag ctccgaccgg atccatgcct tgtcggacag gagatcccga
    16501 tcggctccct gctgtgaagc cacgtcgtcg gcaatacgcg cgaggcgctg cggttcgtcc
    16561 gccgtctcac gctcgccgga cacttcctgc atggcccgca ggtataactc gatcagcagg
    16621 gactctttcc acttggtcag gacatccggc ccgactgcag cgatatctgc agccgtcagg
    16681 gccagcaact tgcgcaacac ttccggcgtg cccacttcgc gtgcgaacgg cagcaacact
    16741 ttttcatcgt agggatcacg acgaaaggcc gtatgggcca tcagcaaatg gcgatgcacc
    16801 aacatgacca acgtccgcga gtcccgctca tcaaacccca atcgcgcggc cgtttcttcc
    16861 gccaaccgct tcccgacttc gctgtggtct tcttcgtggc cctttcccag atcgtgaagc
    16921 agcaacgcca gatgcagcaa atccttccgt ttgatctccc ggtagacttc accgagcaca
    16981 ccggaatgct cagccaaggc ttcggccttc gccactgcca acagactatg ctcgtcgacc
    17041 gtgtacttgt ggtactggtt gaattgcatc agcccgcgca cgcgcgaaaa ggccggcacc
    17101 agtttctcca ggagatgcgc tcgatgcatc gcctccagtg tccgcgccgt gccgggcccc
    17161 gcgagaatcg acaagaacgt acggccggtc tctggggtat gaaattgctc agccgacagc
    17221 gtctccgcct gacgatgaat gtcttcgagg agcgacggat cgatattcaa tcggcgcgat
    17281 cgcgccagat cgaacagcgt cagcagcgat gccggccgat ccaacacctg agaacgcaat
    17341 tcaaccggca ccgtcagcgc ctcgccgtcg acgacaaagt actgatcgat ccggggagac
    17401 ggcaaccatc gcaccacgcg ctgccacaag gaacgacggc gacaccgctc gacaaatcgc
    17461 atcaacgccg cgtgcagccc catcgtgtgt cgatagtatt gctgcatgaa ttgctcgacg
    17521 gcgagcagat gcggccggtc ggtgaagccc agatgtgtgg ccagccagac ctgctcatcg
    17581 aacgtgagaa tttcctgcgc catgccggcc cgagtgtgga ggaacgagcg gacgcgcagc
    17641 aaaaattctt tcgcctcctt gatcgcccga taatcggctt gcgagagaat cccgcgatcg
    17701 gacaattcac gaatcgtcgc ggtatggaag cgcgcagccc cgatccactg caggagatgg
    17761 agatcccgca acccgccttt gctttttttg acgttcggct ccagaaggta gactgtttca
    17821 ccgaacttct ggtactcgcg ctgccgctcg gtcaccttct ggtcgaggta ggtatcgaat
    17881 ccgtgcgcca cgactttccg gacataccgc gcatgaaact gctggaacag gtcggcgctg
    17941 ccggccagga aacgggcctc catcatcgac gtccggaccg tcagatcggc gaggcccaaa
    18001 tccacacagt cttgaatagt ccgtacgcta tggccgacct ggaatccgct gtcccacaac
    18061 gggtgcagca cctgcttgac cagctcgggc atcaccttcg cggcgtccgg gtgaaacaga
    18121 aacatcaggt cgatatcgga gtacggtgcc aattcccgcc gcccataccc gccgatcgcc
    18181 accaggcaac aatgctgaaa gccagccgtc gtgagcgcct cgccgccggt tctcgccgca
    18241 gtccggtagc ggccgacgat caatccatcg accatgtcgg tggtcgccgt gaccacctcg
    18301 tcgcccgagg cgcccgcgag cagccgttgc cggatcgcct gtcgttgctc gaccagcagc
    18361 tgggataccg acgcgggggc cgcgtcctgg gctgtatgag aatccgcatt cacagcgtgc
    18421 gacataccta gagggcgctt tcccccgtct cgcccgtacg aatcctgacg gcggaggtga
    18481 gttcccgcac aaagattttc ccgtcgccga tgctgccggt tttggcggca cgcgtaatcg
    18541 tttcgagcac acgctgcacc tggctgtcat tcacggcgac ctcgatcttc accttcggca
    18601 cgaactcgat ggtgtattcc tggccgcgat acgtttcttt atggcccttc tggcggccga
    18661 agcccttaac ctccgtgacc gtcatgccct gaatgccgat ttccagcagg gcgtccttca
    18721 cctcgtcgag cttgaatggt ttgacgatgg cttcgattaa tttcatcctt gcctccaggg
    18781 agtagctctc agccgtcagc agtcagctct caggcgagga aagagtcctg acaatcttgc
    18841 tgacgactga tggccgattg cgctgagctg gcttcacgaa tacgcccgct cgttatgttg
    18901 gctcaaatcc aaaccggtcg cttcttcctc agacgacacc cgtagcccgg tcatagaatc
    18961 caccagcttg agaatgacaa acgtgccgat gatcgagaaa atcgtcgtga cggccgcgac
    19021 caggagttgg atgccgaaga ggccggggtt accaaagaac aatccatcgg cgccgcccgg
    19081 attgacgacc ttgctggcga acagtcccgt cgccagaatc ccgatcaccc ccccgactcc
    19141 gtggattccc acgacgtcaa gcgagtcatc atagccgaat cgccctttcc agacaatcgc
    19201 ggcgtagcac gtcatgccgg cgaccaaacc gatggcaatg gccgacatgg gtgcgatata
    19261 gccggccgcc ggggtcacgg tcgccaatcc tgccaccgcg ccgctggcga ctccgagcac
    19321 cgtgggtttg ccgcgatgcg cccattcggc gccgcaccag gcaatggccc ccatcgccgc
    19381 cgctgcatgg gtcgcgataa tcgcggagac ggcgatcccg ttggcgccga gtgcgctgcc
    19441 tccattgaac ccaaaccagc cgaaccagag gaatccggtt ccgagcaatg tcatcggcag
    19501 attgtggggc gccatataat ccgtcccgta ccctcgccgc ttccccagca caatggcgca
    19561 gaccagggcc gccgccccgg aactgatatg gatcaccgcg ccgccggcga aatccaaggc
    19621 acccagcttg gccagccagc ctccgcccca gatccagtgc gccaggggca catagacgaa
    19681 caccgaccac aaggccgcaa acaagatgac cgacgtaaac cgcttcctct cggcaaacgc
    19741 gccggtgatc aacgccggtg cgatggcggc gaacatcaat tggaacaaca tgaaggcctg
    19801 gtgcggaatc gtcggtccat agaccggatg gggctcacta cccaccccgc tgagcccgac
    19861 ccattccaag ccaccgatca ccccgccctt atccggcccg aacgccaggc tatacccgaa
    19921 caggatccac agcagactca ccacgctcag gatcatgaca ctttgcatga tcgtgccgag
    19981 tacgttcttg gtccgtacca ggcccccgta aaacagcgcc agtcccggca tcagcatcgc
    20041 cagcacaaac gccgaggaca ccagcaccca tgccgtatca ccggtatcga ccttcagcgg
    20101 cgtattctgc gcccagacct gagtccccca tgactccgcc agcaggatca acacccctgc
    20161 cacgcccacg acgaccgttt tccgctgtgc cgatctcttc accatatcct ccttcggctg
    20221 cgccgctcac cgatcggcct cctccctgac tcagcagagg aggaggcccg ggcgagagac
    20281 taaaacgtct tcgtgaactt caacttatag aaatcctgat tgtctgtcag gtccttaccc
    20341 actgtgaacc ggaaggccat gttccccggc aatttcattt cgacgtacgg tccgatccag
    20401 cgatagtagt cctgagccgc cccgggggca ctgctgtctc tcgtcgtcaa caaatgctcg
    20461 tagtgggccc cgagggacca catgctgttc acgcgcattc cggcactcac ccaataatgc
    20521 aagaagtccc aggtccctcg gccaccggtc tgactggtgc ccaagcaatt cgtgtcgacg
    20581 agtccggtgc aaccggtcgc accgccgcct ccgctgcccc cttcactccg gatggctttg
    20641 tagtagccca gatagaactc gccttcgaag cgatcgtccg tataattcgc gataatgttg
    20701 ggaacgacgc cttcaaacgc cgtcgttcgt tcgttcacac tcccaccacc attgggaaag
    20761 aagccgccgc gagaagagag caattgcccg tgcacgaacc caatcatagg agtgaccgat
    20821 aaccgctttt ccaggaaggt aaaattcaag cccatatcag tttcaagcca gggcgcgttg
    20881 tcgtgcacac ccacgccgtt gatcatggtc caataggtga agttgaaggc cagggcgatg
    20941 ttgtcggtca gtccataggt cccgtagatg gagggattaa acccgaagaa actgtcggct
    21001 tgcatcgcca tggtcaccga cacggggtgt ttggtcgtat ccatcaggtc gtctgtcgtc
    21061 ccggcttgcg cctcgccgag cgccccacct ccgccccccg caagtaatgc caaccccaac
    21121 acccatgtcc gtgcctttcg gccaaccagc atattcatga cgcctccaaa gagagaagaa
    21181 gtgaaagtgc ccgctcagaa gaccgtcgta cagaacggtc tgtgagcccc gtctccctgg
    21241 cgccattgcc agtgttgccg accgacgtcg ttgtcggtcc tgatgcggcc cgtactaccg
    21301 gtacccgttg gtgatcggca tccgccgatc cttcccgaac gctcgatgcg taattttgat
    21361 ccccagcggc gcctgccgcc gcttgtactc acttcggtcc accagcgcga tcacccgcgc
    21421 gaccgtctcc cgatcgaacc ccatcgctgc aatgtcttcc aacgcgcggt cctcctcgac
    21481 gtaggccttg agaatcggat cgaggatggc gtagggcggc aaactgtctt cgtccttttg
    21541 atcggggcgt aactccgcgg tcggagcccg ctcaagcact cgcttcggaa tcaccggggc
    21601 cggcccgacc tgattgcgca tgtacgacag ctcgtagacc atggtcttcg gcacatcctt
    21661 aatcaccgca aaccctccgg ccatatcgcc gtacagcgtc gcgtatccga cgctcatttc
    21721 actcttattg ccggtggtca gcaccagatg accgaactta tttgaatagg ccatcagcag
    21781 attgcccctg atccgggcct gtagattctc ttcggtcgtg tccgggcgcc gtcccttgaa
    21841 ggatcggctg agggcgcgca ggtaactctt gaacgtcgcc gtaatggaca gggtatcgac
    21901 ctgaatgtgc agccggctgg ccagatcggc gacatcttcg cggctggctc tggacgtata
    21961 gggcgagggc atgaacaccc cgagcacatt ctcagcgccc aaggcgtcta ccgcgatgac
    22021 cgctgtgatg gccgagtcca ccccgccgct caatccgatg acgacgcgct tgaacccgtt
    22081 cttgcggaca tagtcctgca ccccgaggac caaggcgcga taggcctcgt cgaggcgacc
    22141 cagcggaggc tccagatccg gcaccacgac cgattgcgac tttctcgcag gcaagcgcac
    22201 atcgacccga tcaatgaggg ccgccacacg cggcggcagg ggtttcttgc ggccctgggc
    22261 cagtcgcgcc cgccccacgg ccgcgacatc gagatccgca acgatgagat cctgatcaaa
    22321 cgccttcccc cgccccgcga tctcgccagc ctggtccaca atcatgctgc agccgtcgaa
    22381 aaccaattca tcctgcccgc cgaccgtgtt ggtgtacgtc acgatcacgc gattttcacg
    22441 ggccctggtc gccagcacct gttcgcgcat ccgtcccttc ccgacatgga acggggatgc
    22501 gttgatattg acgatgacct cggcccccgc tgcagcctgc gcacgggtgg gaccgtcaga
    22561 aaaccagatg tcttcgcaaa tattgacgcc gatcgtcgcg ccgttcagga ccagcagcgg
    22621 cagacgggag ccggggtgga aataccggct ctcgtcgaac acgccgtagt tcggcagatg
    22681 ccgtttccgg taggtggcca gaatccggcg atcggacagc accgccgcct cgttgtataa
    22741 ctcatgccga ccggacagcg ggaacga
//
LOCUS       DAORRQ010000065        22567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_139_length_22567_cov_46.007152, whole genome shotgun sequence.
ACCESSION   DAORRQ010000065 DAORRQ010000000
VERSION     DAORRQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22567
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139_length_22567_cov_46.007152"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="PK224_16545"
     CDS             <1..1362
                     /locus_tag="PK224_16545"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRB17406.1"
                     /translation="VEDEERMRRLFELVLKPAGYQLLLARSGDEALRVIQEHESLDMI
                     ITDLQLGAVSGMDVLEAARQQVPDVPVLIVTGYGTVKSAVEAMQKGAYDYISKPVDNE
                     ELKIVIARALQVRQLARDNRILRAGLHEQFGFDRIVSVSKEMELIKRLAREVAQTDAT
                     VLITGESGTGKDLLARAIHLVSPRAQGPMVALNCAGIPEHLLESELFGYEKGAFTDAK
                     KSKPGRFQMADRGTLFLDEIGELSLTAQAKLLRVLEQHVVEPLGGVRSVAVDIRVIAA
                     TNQELPDLIKAGRFRLDLYYRLNVYQLRMPPLRERPEDIEPILTQFLGQARRERGSRI
                     KGMTAEALTILKQYPWPGNVRELHNVVEWLTITCKQDEITPEHLPASFKTAPPTNEEK
                     PAGTPSLLALGLSVEEVEKTMLQEALRKTGGNVSEASRLLKITRNTLRYRMAKHNLSL
                     PPG"
     gene            1362..3299
                     /locus_tag="PK224_16550"
     CDS             1362..3299
                     /locus_tag="PK224_16550"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRB17407.1"
                     /translation="MRRLRRTGGLQRKFFIALLIVGIVPGMVALWATYRSSTATLKQA
                     IGEGFQEIARSTSIRLATAVDNEIDRAIRLALVPLHVRQPVVLANHQAGATADKTQHQ
                     PSPAHPKSNNPPAVDQDTTSYLHEWADESRHYVRVTIADRQGQVVASTDPRLPPQQAG
                     EVWWREAIQAAPGTSYVSNVTFDPQVNDMVFHVAVPIVDDTRQATIGVVDLLIRRNLL
                     TQMILPIQIGNTGHAMLLDTQGTPLICPVLPPTAHLIPSALMNRLTLDRPLWLVAEND
                     AHGGRDAIVGAAPVRLTHPLTPGSLDGNRWYAFIRQAPEETYAPIYSLLLTVGVIGFG
                     LVIVLSSFGFLVGYRIVTPIVALQREAEGLRLTLALPENGNANRPLTPLLSPGYQTGD
                     EIEDLATSFAAMREVLEENLRTIRSQQHELIRQEKLASVGQLLAALAHDLRNPLGVIR
                     SSAQVVQEGPQEEPIRQEMARYIIDEVDKLSLRLHDFLRYARQKPPDFKVEPAEAVVR
                     AALRQWEAQGGHERIRVEERFEHALPSIQVDPEQVKEALMNLLINAREAMPEGGTLTL
                     TTRAGQDSEVEIEVADTGNGIAPEHLSRIFEPFFTTKAYGTGLGLTNVKRLVEDNGGT
                     LVVNSNAGAGTSFTLRFRAAG"
     gene            complement(3770..4636)
                     /locus_tag="PK224_16555"
     CDS             complement(3770..4636)
                     /locus_tag="PK224_16555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRB17408.1"
                     /translation="MRNNRIWMTSLMALSMTVAGCAEVQRAAEDVARQSGNPRLAGAI
                     HGAGNVVGSLFPIGYEEESSIGQAIALQVVARYGGVVDQPELVRYVNLVGRAVANTSD
                     RPDIPYHVAILDHESINAFAAPAGYIFVTRGLLRQIKNEAELAAVLGHEIAHVSEKHI
                     LDVIQRSKRLAGVTEAGLSYATSNPAAFKGVIDGAVKKLLDEGLDQEKETEADTVGEV
                     FAARVGYDAEAYVGLLTRLRDLKGDDRALFKTHPNFSARIEGVQKTIRTKRLVSNGLV
                     LQERFLRMTKRV"
     gene            complement(4626..5138)
                     /locus_tag="PK224_16560"
     CDS             complement(4626..5138)
                     /locus_tag="PK224_16560"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRB17409.1"
                     /translation="MIPPRSFWIPLIALWIGLFVWGTEAWAETVYVQAKTAQLRAGKT
                     SLDAVVGNVKFGDALEVVGRDGSWMEVKTSAGARGWIFANKTSTSKPSGGNDTLARLG
                     QSMRGGDASATTASAGARGLDKASEGYADRAGVSARDREVVDRMTAYQIPDQDVEEFM
                     REGGLGEYAK"
     gene            complement(5165..7303)
                     /locus_tag="PK224_16565"
     CDS             complement(5165..7303)
                     /locus_tag="PK224_16565"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRB17410.1"
                     /translation="MTSTPFQRKILSAVAVGLTVFALVAGLSTSRWFEVAELKALDHL
                     VRRYADPAKANSNLVLLAIDESSLEAFGRWPWPRDRFGYVVRYLKQAGARAVVFDVMF
                     FEADENAEEFDQSFADDLKAAGNVFLPMLFQAEPAAIPPEAQPRATVTVESSDARQAP
                     DTHAGVKLPIPVLAQQARGLGVINLSADADGPTRRVPLLGQVNGNVVPHLSVAVAQYL
                     LGADRLSMRDGRLQIGAHHVPLETDGSLLIDWHGSLEQTYHAKKYSIGRVLQAFAQQE
                     KGERPSLDPSLFKDKVVFIAGTAAGLYDLRVTPFSAATPGVLIHMAALDNLLHGQGLQ
                     AAPRWFSLTTLLLLCLASAGTFMLFRSYPVKFGVTIGLAVAYYGLVVHAFAGHERWLE
                     LVFPEAALALTFGTAATVEYVTEGKQRRLMRAAFDKYMSSEVVEEIMRNPEAIKLGGE
                     KKEITIFFSDIAGFTTISEKMSPEDLVTLLNRYLSAMTTIIKTTHRGNVNKYLGDGIM
                     ALFGAPLDDPKHASLACYAALDCQVELARLREVWKRERLPEIGARIGLNSGPCIVGNM
                     GSEERMEYTVTGDSVNLASRLEGASKYYDTLILIGQRTAELAKNDVEVREIDLLRVKG
                     KKEPVVVFELLARKGQLDDKKRHVIDVYLEGLAAYKMRNFSTACARFSEAVALDPSDG
                     PSRVYLERSTNYGQMPPPGEWDGVYEMTSK"
     gene            complement(7583..9196)
                     /locus_tag="PK224_16570"
     CDS             complement(7583..9196)
                     /locus_tag="PK224_16570"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17411.1"
                     /translation="MKIYLVLLVLLFAPVESGRANERPPLPVEKPAGSGVIVHRDGYV
                     LTAHHVVANAKRITIVTSGEFRAPAVLVSVDAEHDLALLKVETVGLSEALLGYAGAVK
                     LDQEVIAVGFQFGLREITITRGHVAAVRTKGVQRVFQVDAAVNPGNSGGPVFNRKGEV
                     VGILTTKFTHPSGIVPEGMAFAVPISYATPLLANIPDFDFSAIGRGRRGSPKGKGNGD
                     LVTELTRTSVRIETVRMSEGPATVIHPAPMLPSSDGRHSAQPSVTRVPVPPAPVHEPV
                     PLAGDESIDRVNAQLLASQQEELKRFVDQGLTPPGEMVLIPAGEFLMGMEDGLPDARP
                     IHRLYVSAYWIDRHGVTNGQYRACVEGGACLIPKVRDAFDDTQRAQFPVTNVTWSQAR
                     AYCQWVGKRLPTEAEWEKAARGIDGRRYPWGNSDEVIQKSRVKLTDGNSANGVDPLGL
                     PSVSASPYGVFGMIGMVSEWVKDWYAEDFYRTSPARDPQGPLRGTFRVLRGGSWMERP
                     LELRASYRGWDEMTYWGPTLGFRCATDVP"
     gene            complement(9193..9972)
                     /locus_tag="PK224_16575"
     CDS             complement(9193..9972)
                     /locus_tag="PK224_16575"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB17412.1"
                     /translation="MRRLPWMLACLVAAGWPSALPFAWGLESSDRSTIPFHTILELEQ
                     TARLLAILLDSGRAVVNDNQTLLDDPTKAHKGFTPEAFEGQLRDMFRGRAGINLDELA
                     GGTLSPRSRHLLQELVTVSKQVIADAQAELNRQGAGFKGFIPAVFGARVSTRFTAVTG
                     VRLKQTALDPRNPANRPDSYERVALEAFADSAYPREKVISEMAANSSSLRLMFPLYAT
                     RQCLDCHGGPKGGLDRTGYPREGLTLGQNAGAISVLLPVGK"
     gene            complement(10115..10624)
                     /locus_tag="PK224_16580"
     CDS             complement(10115..10624)
                     /locus_tag="PK224_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17413.1"
                     /translation="MRWKACLVVAAIGVFFPVASGWADSTDSPLMGVRSAVSGQIVKI
                     ESGLLFVKTPYGLQFRTISPNKADRVGLHNARLGDEVWLLIDSGNVLLDAARPGGEHF
                     ANHKVVAGRIHYADPFWGEIQISTPEGFERFEVDALAGSKLSVFQEGMPVTIELDSDN
                     VMIDIQSGR"
     gene            complement(10665..11060)
                     /locus_tag="PK224_16585"
     CDS             complement(10665..11060)
                     /locus_tag="PK224_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17414.1"
                     /translation="MTIQNRGYGICALVFGLCVGLALLAGSVSAQQSWVDEINNSLNF
                     YKTSYPGSNWEPYSQRLQTVKEAVDRADTRTVKSEMGKWFKMLRAREQGIHDVAADEL
                     FNFALMVTPILEYGITVPPAPGGGTEPGY"
     gene            complement(11097..11426)
                     /locus_tag="PK224_16590"
     CDS             complement(11097..11426)
                     /locus_tag="PK224_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17415.1"
                     /translation="METGSRIKSVCVIVGGLGLGLFIASGGQAQVIHPDQMIGVPSPQ
                     TEIGSGESPTSSEPSVNDMEKGFDRHMMVPESGASGSMDTNKLKTPDATDAENARESD
                     RQSSVSF"
     gene            complement(11582..12226)
                     /locus_tag="PK224_16595"
     CDS             complement(11582..12226)
                     /locus_tag="PK224_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17416.1"
                     /translation="MPITIVIAEDQRLFRQSLRLLLEHERDLVVVGEAMDGRQAFDLA
                     VAHKPGIVLMDVDMPRLDGITATRLIRSCVPDSKVLMLSVHDDDARIVAAVQAGACGY
                     ILKDADHQEFLRIIRATFEGDVVRSPFMPDGFAKKAMALVNKQGESEGGGLSLLTERE
                     HEILACAAAGRSNKEIADQLCVSLDTVKTHLHHIYQKLRVSGRVEAVLTYLQSR"
     gene            complement(12208..14514)
                     /locus_tag="PK224_16600"
     CDS             complement(12208..14514)
                     /locus_tag="PK224_16600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB17417.1"
                     /translation="MRQRVSLQSLITLSVLSVGILSGTVGLVYAYWHAKQSLHTTVGI
                     TFQEIARQSADKAALLLAKELEWMQRLSALPEIRASVEGVSSAKQPTLQFEQWREEQH
                     RYFHSLVIVRWDGQLVGGRQSESARTFYAQQPWWPVVFSAQRAWAGPLTVDDNGRGFW
                     EVAVPIGGQGQPVRGALKAVIGTDRILSSIVGSRIGRTGHMMLLAEDGVVLACSMLAP
                     NLHATLQTQSMQAPADAPAARWFQLEQDTHGGNQSIIGLARVVLPAQIEQARGWSILM
                     QQDPSETFEPLLALMGKLGAFWVGAVVFIVWLRWRLARRIVRPLGDLIQRVNVFGGAW
                     PSEPLPSQAFSRPSGIVEIDALAASFDDLAQRLEQASRVKAQYVRRLEQVNLDLATSE
                     EHYRLLWNHSVDTKILIDLFGSILDINRRGEVTLGRPASTLARRPFTDLVAESERPRL
                     LEQLESVIRSGADLSGGAMHVPIPTGDLTMEVDWVPVKKAGRIESIMIQLRDLTEQKA
                     LERQVVRSERLASLSQFASMFAHDIRNPLAGIKKTLEWLGGCPEMGQDPPRRCLEDLR
                     FTTDLLLGMINDMLDVYQENYSGLPLSTSPVSPSLLMHEVLRLFRSEAEAQEVRFNVQ
                     VPSEAVWIMGDGRRLQRVLINLIHNALKYSPARGAIMISVQVNGGQVSQGETVAGPDA
                     CSTVTIQIQDEGPGIAPEDLPHLFEMFFRKKDGQDYRIGRGLGLHFCRLVVDAHGGQI
                     KAANRPEGGAVFTVALPVRQEQVCPSPS"
     gene            complement(14705..17137)
                     /locus_tag="PK224_16605"
     CDS             complement(14705..17137)
                     /locus_tag="PK224_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRB17418.1"
                     /translation="MQSETLQSESCNVLEWAKLLEHLASYAQSAIGTERCRSLVLETD
                     LTEAQTRQQETSDMLRLRSGVDPFPVLRFPNVAEWLGRVAKGACLEAHELRDIALVLG
                     LIDDVQRYLLRHQADAPAVGAMVVQLGPVEEYRRLTIALNAAIDPDGSMRESASPELH
                     RLMQRANDLKRQMRHRLEQILHSRRYEEVLQEHYFAQREGRYVVPIKAEMQGRIPGIV
                     HDVSSSGATVFLEPRELVDLNNSIKVVDLDVEREVRRILRELSAMVAPHQPALAGSVA
                     LLGELDAISAKAGLSQRLQAVPPRLNEQGRILLNRARHPFLVLTKEQVVPNDLVFDET
                     VRVLIISGPNTGGKTVTLKLLGLFALMVRCGLHLPCAPDSEMAIFPSVYADIGDAQDL
                     ARDLSSFSAHITQMVQLLKEVSEGTDVGTSPSPIHPGRALVLLDEPVTSTDPAEGAAL
                     ASALLCRLAAAGVKVVATTHYSLLKGLAQETPGFANASVEFNIDTLSPTYRLIQGQPG
                     GSAAIEIAGRLGMDESLLQDARARLQGDNRLLETLLNDLQDKQRRMSEDLAAATAAKQ
                     SAEQAARESQELLAFLQESERDERQGLKKKLSQEFQRARAAVQATLDDLKRDQKLLKA
                     KETKVRLIELEQAVSREVRAAQEPIPVDQLSVGDAVEVVGLGMTGTLLESPQGKKRVR
                     LKVGEGEILANVGSLVGLARPPQPARSEPRKTTTVTSRRTSQPLAPEDIQETLDVRGQ
                     AADDALDIVVAGLDRAIFGGSPFVRIIHGHGTGRLRAVLRDYLKASPYVVTFRPGDRA
                     EGGDGVTIVELR"
     gene            17358..18365
                     /gene="gap"
                     /locus_tag="PK224_16610"
     CDS             17358..18365
                     /gene="gap"
                     /locus_tag="PK224_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRB17419.1"
                     /translation="MTTRIGINGFGRIGRNVLRASLGDPSLEFVAINDLTDAKTLAYL
                     LKYDSVHGTLDASVEAKDDQLIIDGKAIKVLAVRDPKELPWKALGVEIVVESTGHFTD
                     REGAGKHLSAGAKTVIISAPAKDPDATVVLGVNEQVFDAKAHHIVSNASCTTNCLAPV
                     AKVLLENFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHL
                     VIPQLKGKLDGLAIRVPTPNVSLVDLTVETEKDCDVAAVNAAFKKAAEGPMKNVLAYS
                     DAPIVSIDLKDDPHSAIVDAPLTAVIDKRLVKVTAWYDNEWGYSCRVRDLIKYIVAKG
                     A"
     gene            18462..19661
                     /locus_tag="PK224_16615"
     CDS             18462..19661
                     /locus_tag="PK224_16615"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRB17420.1"
                     /translation="MNIRKRIIEDVQLRGKRVIIRADYNVPLDDSLQITDDTRIRSTL
                     PTINRAVDEGAKVILCSHLGRPKGRFDPKFSLAPVAKRLQRLLGKEVIFAPDCIGAAV
                     EGLVAKMQPGDVMLLENLRFHPEEEKNDDGFSKALASLADVYINDAFGAAHRAHASTV
                     GITKYIPEAAAGYLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKKVDK
                     VIIGGGMAFTFLKAMGLEIGQSLVENDMLDFAKGVQDHAFSSGVKFYLPVDCVVAASR
                     EPGAETKIVPVQEIPKGWYGLDIGPASVKLFSEAVQDAKTILWNGPMGMFEVDAFARG
                     TLAMAHSVANAYALTIVGGGETALAIHRAGESESISFISTGGGAALELLEGKTLPGLA
                     ALPNRAA"
     gene            19701..20480
                     /gene="tpiA"
                     /locus_tag="PK224_16620"
     CDS             19701..20480
                     /gene="tpiA"
                     /locus_tag="PK224_16620"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRB17421.1"
                     /translation="MRTRFIVGNWKMNKTASEAGAFVHRLSQELPHHAGIQIGLTPPF
                     TALHAVREALGPASAVLLGAQNLYWEDKGAFTGEVSGAMLKDIGCQFVLVGHSERRQY
                     FGDQDSWTNKKVLAALRHGLQPILCVGETLQDRDSGQTDLVIQRQLRAGLAGTEAHTL
                     PALTIAYEPVWAIGTGRAASPEQAVPVHRLIRRTIGELSGTDIGNHVRILYGGSVTPH
                     NIADFLASEEIDGALVGGACLDPVSFATLVKVAIGSKPTTA"
     gene            20490..20843
                     /gene="secG"
                     /locus_tag="PK224_16625"
     CDS             20490..20843
                     /gene="secG"
                     /locus_tag="PK224_16625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRB17422.1"
                     /translation="MYTLLIIIHVIVCLLMIGAILLQSGKGAEIGAAFGGSSQTVFGS
                     RGPANFLSKFTVITAFVFMFTSLSLAIIAKDRNFASTVIDLNKKPAATTPSSTPSETT
                     TPPAAESHSSGEGSH"
     gene            complement(20987..21901)
                     /locus_tag="PK224_16630"
     CDS             complement(20987..21901)
                     /locus_tag="PK224_16630"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HRB17423.1"
                     /translation="MLQTSEKIWMDGKFVAWADAQVHVLTHSLHYGLAAFEGIRCYKG
                     KSGSAIFRLPEHVDRLFESAHIGLIEMPFDRKQISEAIVETVRVNRLEACYIRPLVYI
                     GYGAMGLYPAENPIKVSIAAWKWGTYLGDEALSKGIRARVSSFTRHHVNVSMTRGKIS
                     GYYVNSILAKREVKADGYDEAIMLDPEGYVAEGTGENVFIVRRGVLKTTPLTSILEGI
                     TRNSIIQLAKERNIPVVEERFTRDEMYVAEEVFVTGTAAELTPVTEIDQRRIGSGKPG
                     PITQTLQKAFFDVVGGTDSAHRQWLTPV"
     gene            22111..22425
                     /gene="rplU"
                     /locus_tag="PK224_16635"
     CDS             22111..22425
                     /gene="rplU"
                     /locus_tag="PK224_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRB17424.1"
                     /translation="MYAIIETGGKQYRVETGTVLQVESLPGDVGHSVQIDKVKLLHGE
                     GGLVVGQPVVAGATVTAEIIRQGRTRSITVFKKHRRKNYRRTRGHRQGFTQLRVTGIE
                     TK"
     gene            22447..>22567
                     /locus_tag="PK224_16640"
     CDS             22447..>22567
                     /locus_tag="PK224_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRB17425.1"
                     /translation="MATNKGGGSTRNGRDSNPQYLGVKAYGGETIKAGSIIVRQ"
BASE COUNT         4772 a         7275 c         6252 g         4268 t
ORIGIN      
        1 gtcgaagatg aagaacgaat gcgccggctc ttcgagctgg tgctgaaacc tgccgggtat
       61 cagctgctcc tggcccgctc gggggacgaa gccctccgcg tgatccagga acacgaatcc
      121 ctcgacatga tcatcacgga tctccaattg ggcgccgtct cgggcatgga tgtgctggaa
      181 gcagcccggc aacaggtgcc cgatgtgccg gtccttatcg tgaccggata cggcaccgtc
      241 aaatcggccg tcgaggccat gcagaaaggc gcctacgact acatctccaa gcccgtcgac
      301 aatgaagagt tgaagatcgt cattgcccgc gcgctgcagg tccgccagct cgcccgggac
      361 aatcgcatcc tgcgcgcagg gttgcacgaa cagttcggct tcgaccgcat tgtcagcgtc
      421 tccaaagaga tggaacttat aaagcggctc gcccgggaag tggcgcagac cgacgccacg
      481 gtgctcatca caggcgaaag tggaacgggg aaggatctgc tcgcgcgcgc catccacctc
      541 gtcagcccac gcgcccaagg cccgatggtg gctctgaact gcgccggcat ccccgagcac
      601 ctgttggaat ctgaactgtt cgggtatgaa aagggagcgt ttaccgacgc gaagaaatcc
      661 aaaccgggac gctttcaaat ggcggatcga ggcacgctgt tcctggacga aatcggcgaa
      721 ctgagtttga cggcacaggc caaactcctg cgcgtcctgg aacagcatgt cgtggaaccg
      781 ttgggcggag tgcgcagtgt cgccgtcgat attcgggtca tcgccgcgac caaccaggaa
      841 ttacccgatc tcatcaaagc cggccgtttc cgcctcgatc tctactatcg actgaacgtc
      901 tatcagctcc ggatgccgcc ccttcgcgaa cggccggagg acatcgagcc gattctcact
      961 cagttcctcg gccaggcccg tcgggaacgc ggcagccgca tcaaaggaat gaccgccgag
     1021 gcgctcacga ttctcaaaca gtatccctgg ccgggcaacg tgcgagaact gcacaatgtc
     1081 gtggagtggc tgaccatcac ctgcaaacag gacgagatta cgcccgagca cctgccggcc
     1141 tcgttcaaga ccgctccgcc gacaaacgaa gagaagccgg ccggcacacc gtcgctcctc
     1201 gcgctgggac tctccgttga ggaagtcgaa aagacgatgc tccaagaggc gttgcgcaaa
     1261 accgggggga acgtgtccga agccagccgt ttgctcaaaa tcacacgcaa cacgttgcgg
     1321 tatcgaatgg ccaaacacaa cctgtcgctg cccccaggct gatgcgtcgc ctacgacgaa
     1381 ccggcggact ccagagaaaa ttcttcatcg cgctgctcat tgtcggcatc gtgcccggca
     1441 tggtcgcgct ctgggcgacc taccgctcca gcaccgccac cctcaagcaa gccatcggtg
     1501 aaggattcca ggaaatcgcg cgttccacct ccattcgcct ggcgaccgcc gtcgacaatg
     1561 aaatcgatcg cgccatccgg ctggcgctcg tcccgctgca cgtccgtcag ccggtcgtcc
     1621 tcgccaacca ccaggccggt gccacagccg acaagacaca acatcagcca tcaccggctc
     1681 atccgaaatc caacaacccg cccgcggtgg accaggacac cacaagctac ctccacgaat
     1741 gggcggacga atcccggcac tatgtgcgtg tcaccattgc cgaccggcag ggtcaggtcg
     1801 tcgcctcgac agatccgcgg ctaccgccgc aacaggcggg cgaagtctgg tggcgtgaag
     1861 ccatacaggc cgcacccggc acatcctacg tcagcaacgt gacgttcgat cctcaggtga
     1921 acgacatggt cttccatgtt gccgtcccga tcgttgacga cacccgccag gccaccatcg
     1981 gtgtcgtcga cctcctcatt cgtcgcaatc tgctgactca aatgatcctc cccatccaga
     2041 tcgggaacac gggtcatgcc atgctgctcg acacgcaagg cacaccgttg atctgcccgg
     2101 ttctgccgcc caccgcccat ttgattccat cggccttgat gaaccgactg acgttggatc
     2161 gccccctgtg gctggtcgca gagaatgatg cacacggcgg ccgcgatgcg atcgtcgggg
     2221 cggcgccggt ccggctgact catccactga cgccggggag cctcgacggc aaccgatggt
     2281 atgcgtttat ccggcaggcg cccgaggaaa cctatgcgcc gatctattcc ctgctgctga
     2341 cggtcggcgt aatcgggttc gggctggtga tcgtgctctc ctccttcggg ttccttgtcg
     2401 ggtaccgcat cgtgacaccg atcgtggcgc tccagcgcga agcggaaggc cttcgcctca
     2461 cactggcctt accggagaac ggcaacgcga accggcccct taccccattg ctttcgccgg
     2521 gctatcagac cggtgatgag attgaagatc tggccacgag tttcgccgcc atgcgggaag
     2581 tgctggagga aaatcttcgg acgatccggt cacagcaaca tgagctgatc cggcaggaaa
     2641 agctggcgtc tgtgggacaa ctgctggccg ccctcgccca tgatctgcgg aacccgctcg
     2701 gcgtcatccg gagttccgca caagtcgtgc aggaggggcc gcaggaagaa cccatccgcc
     2761 aggaaatggc tcgctatatc atcgatgaag tcgacaaact ttcactgcga ctccacgact
     2821 ttctccggta tgccagacag aagccgcctg acttcaaggt agaacctgcg gaagccgtcg
     2881 tccgcgctgc gctgagacag tgggaagcac agggtggcca tgaacgcatc cgggtcgaag
     2941 agcgatttga gcatgccctg ccctcgattc aggtcgatcc ggagcaggtg aaggaggccc
     3001 tgatgaacct cctcatcaat gcgcgtgaag cgatgccgga aggaggaacc ctgaccctca
     3061 cgaccagagc cggacaggac agcgaggtgg aaattgaggt ggccgatacc ggaaacggca
     3121 tcgctccgga acacctttca cgaatcttcg agcccttctt cacgaccaaa gcctatggaa
     3181 ccggtctcgg cctcaccaac gtcaaacggc tcgttgaaga caacggcgga accctggtcg
     3241 tgaacagcaa cgcaggagcg ggaacatcct tcaccctgcg ctttcgtgcg gcgggctaga
     3301 aacgcacaag ggatatcaac ggcggaaaat ctaccattgt tttctagccg gccgttcaac
     3361 atggccgaca gcggggccgc agcaagcgac gggacgaggc gtacccttgc ggtacgtgaa
     3421 gcccctgagc gacgcgagaa caaagccagc ggtaatctca gtagtcacgg aggaggctca
     3481 aacaggctgt ccagcaaggc cgcagcgagt gaggagacga gtcgtaccct tgcggtacgg
     3541 cgagggccgg aacgacgcga gaacgacgtt ggcggcctgt ttcagcctcc tgctagcccg
     3601 gattattcat gaatgccgtc gcgaggtgtt cgagacggaa gcccgccgag gctgctcgca
     3661 cgtaaggccg acgcaggcgt actggcagtc cgtcgaggag gccgaacgag aacagcctcg
     3721 ccgggcgaca ggatcggacg ccggagcagg gattcatgaa tagttcgggc tagacgcgtt
     3781 tggtcatgcg gaggaaccgt tcctgcaaca ccaagccgtt cgatacaaga cgcttggtgc
     3841 gaatggtttt ctgcacgccc tcgatccgcg cggaaaagtt gggatgggtc ttaaacagcg
     3901 cacggtcatc gcccttgaga tcgcgcaacc gcgtcagtag gcccacatag gcctccgcat
     3961 cgtatccgac ccgcgcggcg aacacttcac cgaccgtatc ggcctctgtt tctttctcct
     4021 gatccagacc ttcatcgagc agcttcttga cggcgccgtc gatcacgccc ttgaacgcag
     4081 ccggattact cgtcgcatag gataggccgg cttcggtcac tccggccagg cgcttgctgc
     4141 gttgaatcac atcaagaatg tgcttttcgc tcacatgcgc aatttcatgc cccaggacgg
     4201 cggcgagctc cgcctcattc ttgatttgcc tgagcaggcc ccgggtcaca aaaatatatc
     4261 cggcgggcgc ggcaaacgcg ttgatcgact cgtgatccag aatggcgaca tggtatggaa
     4321 tatcgggccg gtcggacgtg ttggccaccg ccctccctac caggtttaca tagcgaacta
     4381 attcgggctg atccacaaca ccgccgtacc gtgccacgac ttgcagggct atcgcctgcc
     4441 cgatcgacga ttcttcctcg tagccgatgg gaaacagact gccgacgaca ttgcctgcgc
     4501 catggatcgc accggccaat cggggattgc cggactgtcg ggcgacatcc tcggcagccc
     4561 gctgcacctc tgcacaacca gccacagtca tcgagagcgc catcaggctc gtcatccaga
     4621 tcctgttatt tcgcatattc gcccaggcct ccttcccgca tgaactcttc gacgtcctgg
     4681 tcggggattt gataggccgt catgcgatcc actacctccc gatcccgcgc ggacacgccg
     4741 gcccggtcgg cataaccctc ggacgccttg tccagccctc tggcgcccgc cgatgcggtg
     4801 gtcgcggacg cgtctccgcc ccgcatgctc tggccaagcc gagccagggt gtcattgcct
     4861 ccggacggtt tcgatgtcga tgtcttgttg gcgaagatcc atccacgcgc gccggctgaa
     4921 gtcttgacct ccatccagct tccatcgcga ccgaccacct ccagcgcgtc gccgaacttc
     4981 acgttcccga ccacggcatc cagcgaggtt tttcctgcgc gaagttgcgc cgtcttggct
     5041 tgcacgtaga cggtttcagc ccaggcttcc gtcccccaga cgaacagacc gatccacagg
     5101 gcgatgagcg gaatccaaaa cgatcgtggt ggtatcacag cttcctcccc ctcggcactg
     5161 ccacttattt tgaggtcatt tcatagacgc cgtcccactc tccaggcggg ggcatctgtc
     5221 cataattcgt ggatcgttcc aggtagactc gcgaaggccc atccgacgga tccagcgcaa
     5281 ccgcttctga aaacctggca caagccgttg agaaattccg catcttatag gccgccagcc
     5341 cttccagata cacgtcgatc acgtgccgct tcttgtcatc cagctgcccc ttccgggcca
     5401 acagttcgaa cacgacgacc ggctcttttt tccctttcac tcgtagcaga tcgatctccc
     5461 gcacttcaac gtcgttcttc gccagttcgg ccgtccgctg gccgatcaga atcagcgtgt
     5521 cgtaatactt gctggcgccc tccagacgcg aagccaggtt cacactatcc ccggtcaccg
     5581 tatattccat tcgctcctcg gaacccatgt tgcccacgat gcatgggcct gaattgaggc
     5641 cgatcctggc gccgatctcc ggaagccgct cccgcttcca gacctcccgc aagcgggcca
     5701 actcgacctg acagtccagc gccgcgtaac aggccagcga cgcatgcttc gggtcgtcca
     5761 gcggcgcgcc gaagagcgcc atgatcccgt caccgagata cttgttcaca ttgccacgat
     5821 gggtggtctt gatgatggtc gtcatcgccg acaagtagcg attgagcaat gtcacgaggt
     5881 cttcgggaga catcttctcc gagatcgtcg taaagcccgc gatgtcagaa aaaaagatcg
     5941 tgatttcttt tttctccccg ccgagcttga tggcctcggg attccgcatg atctcctcga
     6001 ccacctcgga cgacatgtac ttgtcgaagg ccgcccgcat gagacggcgc tgcttgcctt
     6061 cggtcacata ttcgacggtc gcggccgtgc cgaacgtcaa ggccagcgcc gcttccggaa
     6121 acaccagttc cagccaccgc tcgtgcccgg caaacgcatg tacgactaat ccatagtagg
     6181 ccaccgccaa tccaatcgtg acgccgaact tgaccgggta ggaacggaac aacatgaacg
     6241 tgccggcaga agccagacag agcagcagga gggtcgtcag cgagaaccac ctcggagctg
     6301 cttgcaagcc ctgcccgtgc aggagattgt ccaacgccgc catatgaatg agcacaccgg
     6361 gcgtggcggc agagaacggc gtgacacgaa gatcgtagag ccccgccgcc gtaccggcga
     6421 taaacaccac cttgtccttg aacaacgacg gatccagcga cggccgttcg cccttctcct
     6481 gctgcgcgaa ggcctgcaac acccgtccga tcgaatactt cttcgcatgg taggtctgct
     6541 ccagggatcc atgccagtca atcaacaggc tgccgtcggt ctccaatgga acgtggtggg
     6601 cgccgatctg cagacgcccg tcacgcatcg agagccggtc ggcgccgagc aggtattgcg
     6661 ccaccgctac cgagagatgc ggcacgacat tgccgttgac ctgccccagc agcggaaccc
     6721 gcctcgtcgg tccatcggcg tcggcagaca ggttgatgac acccagcccg cgcgcctgtt
     6781 gtgccagcac cggaatcggc agcttcactc cggcatgagt atccggcgct tgtctcgcat
     6841 ccgacgactc caccgtgacc gtggcgcgag gctgcgcctc aggcgggatc gcagcgggct
     6901 cagcctggaa cagcatcggc aggaacacgt tgcctgccgc cttcaggtca tcggcaaacg
     6961 actggtcgaa ttcttccgca ttctcgtccg cctcgaagaa catcacatcg aacacgacgg
     7021 ctctggcgcc ggcttgcttg agataccgca cgacatagcc gaatcgatcg cgaggccagg
     7081 gccagcgccc gaatgcctcg aggctcgatt catcgatggc gagtaacaca agattcgaat
     7141 tggctttggc aggatccgca tagcgtcgga ccaaatgatc cagcgctttg agttctgcga
     7201 cctcgaacca gcgggaggtg ctgagcccgg ccacgagggc gaacaccgtc aggcccaccg
     7261 cgaccgccga aagaattttt ctttgaaagg gagtggaggt catgaaggcg attgcacgat
     7321 caaagacacg ctgaccatcg ggctatcgta cgtgagaacg aaccggaggg aaagggggct
     7381 ggagggggcc tttttcagca cccggctggc ccggatgatt cctgagggcc gtcgcgaggc
     7441 gttcgagacc gaagcccgcc ggggcttctc gcaagtgagg ccgacgcagg cgtacttgga
     7501 gtccgtcgag gaggccgaac gagaaaagcc ccgccgggcg agaggatcgg acggcggagc
     7561 aggcagtcag gaatcttccg ggctagggga cgtcggtcgc gcaacgaaat cctaacgtcg
     7621 gcccccagta cgtcatctcg tcccagcccc ggtagctggc gcgcaactcg agaggccgct
     7681 ccatccagct gccgcctcgc aagacacgaa acgtcccgcg caacggcccc tgcggatccc
     7741 gggccggcga cgtgcggtaa aaatcctccg cataccagtc cttcacccat tccgacacca
     7801 tcccgatcat gccgaacacg ccgtagggtg acgcggagac tgagggcagg cccaacggat
     7861 cgacgccatt ggcagaattc ccgtccgtca gcttgaccct gcttttctga atcacctcgt
     7921 cgctgttacc ccagggatat cggcggccgt cgattccccg cgccgccttc tcccattccg
     7981 cctccgtcgg caatcgttta ccgacccact gacagtaggc ccgggcttga gaccaggtga
     8041 cattggtcac cgggaattgg gctcgctgag tatcatcgaa ggcatcgcgc actttcggaa
     8101 tcagacatgc gcctccctcc acacaggccc ggtactgccc gttcgtcact ccatgccggt
     8161 cgatccaata ggcactcaca tacaaccgat ggatcggacg ggcatcgggc aaaccatcct
     8221 ccatgcccat gaggaactcg ccggcgggaa tcaacaccat ctccccggga ggtgtcagtc
     8281 cttgatcgac gaatcgcttg agctcttcct gctggcttgc cagcagttga gcattcacgc
     8341 gatcaataga ttcgtctccg gccaggggaa ccggctcatg gacaggagcg ggcggcactg
     8401 ggacacgggt gacggacggc tgggcactgt gccggccatc ggaagacgga agcatcggtg
     8461 cggggtgaat gaccgtggcc ggcccttcgg acatgcgcac ggtttcgatc cgcacggacg
     8521 tccgcgtgag ttccgtgacc agatcaccgt tgccctttcc cttgggcgac ccccttctcc
     8581 ccctgccgat ggcggagaaa tcgaaatccg ggatgttggc taacaggggc gtggcataac
     8641 tgatcggcac ggcaaaggcc atcccttccg gcacgatccc ggacggatgg gtaaacttcg
     8701 tagtgaggat tcccacgact tcgcccttcc ggttaaatac cgggcctccg ctgttgccgg
     8761 gattcaccgc ggcatcgacc tgaaagaccc gttgcacgcc cttggtcctg acggcagcca
     8821 catggccacg agtgatggtg atttcgcgta acccgaactg aaacccgacg gcaatgacct
     8881 cctggtccag cttcacagcg ccggcatacc cgagcaacgc ctcggagagc cccaccgtct
     8941 ccaccttgag caaggcgaga tcgtgttccg catccacgct caccagcacg gcgggcgccc
     9001 gaaattcccc ggacgtcacg atcgtgatcc gcttcgcatt ggcaacgaca tggtgggccg
     9061 tgaggacata cccatcacga tgaacaatga cgccgctacc ggccggcttt tctaccggca
     9121 atggcggacg ctcgttcgcc cgtccacttt cgacaggcgc gaacaacagg acaagcagga
     9181 ccaggtagat cttcattttc cgaccggcaa cagcacgcta atggcaccgg cgttttgccc
     9241 caacgtgagg ccttcccgcg gatagcccgt gcgatcgagt ccccccttcg gaccgccatg
     9301 gcaatcgagg cactgtctcg tcgcatagag cggaaacatc aggcgtagcg acgaactgtt
     9361 ggcagccatt tcactgatga ctttttcccg ggggtaggcg gagtccgcaa aagcctccaa
     9421 tgcgacccgt tcgtacgagt caggccgatt ggcaggattc cggggatcca aggcagtctg
     9481 tttgagtcgt acgcccgtca cagcggtgaa gcgcgtggac acgcgcgctc cgaacacggc
     9541 cggaataaac cccttgaacc cggccccctg ccgattgagt tccgcttgag catccgcgat
     9601 tacctgcttg ctcaccgtca ccagctcctg aagcagatgg cggctccgcg gggatagcgt
     9661 accgccggcc aactcatcca ggttgatgcc ggctcggcca cggaacatat cgcgtagttg
     9721 tccttcaaaa gcctccggcg tgaagccttt gtgtgccttg gtcgggtcat ctagtaaagt
     9781 ttgattgtca ttcacgactg cgcggccgga gtcgagcagg atggccagca gacgagccgt
     9841 ctgctccagt tcgagaatgg tgtgaaaggg aatagtcgac cggtcggagg actcaagccc
     9901 ccaggcaaac ggcaaggccg agggccaccc tgctgctacc aggcacgcca gcatccacgg
     9961 cagacgtcgc atgagccgcc tctggtgcat tcgcgttgcg gactgcgaga accgcgcgct
    10021 cctgcccctg attacctggc aggagcgcgc gtcagtaaaa atcgtacgag cctctcacga
    10081 tgaagtcaag gcgacgacac gcacgacagt cacattaccg accagactgg atgtcgatca
    10141 tcacgttgtc ggaatccagc tcgatcgtca caggcatccc ctcctgaaat accgacagct
    10201 tgcttcccgc cagcgcgtcg acttcaaacc gttcgaatcc ctcaggagtc gagatctgaa
    10261 tctcccccca gaaggggtcc gcatagtgaa tccggccggc cacgaccttg tgattggcaa
    10321 agtgttcacc gcccggcctg gccgcgtcca gcaacacgtt ccctgaatcg atcagtaacc
    10381 agacttcatc gccgagccga gcattgtgca gccccacccg atcggccttg ttcggactga
    10441 ttgtccggaa ttgcaaccca tagggagttt tgacaaacag gagcccactc tcgattttga
    10501 cgatctgccc actcaccgcc gaacgcacac ccatcagcgg gctgtcggtc gaatcagccc
    10561 atcctgatgc caccgggaag aaaaccccta tcgcagcgac gactaggcac gccttccacc
    10621 tcataacggc ctcctttcgc accaactcac atccaggcat cgactcaata gcccggctcg
    10681 gtgcctccgc cgggcgccgg tggaaccgtg atgccgtatt ccaggatcgg cgtgaccatc
    10741 aacgcaaagt tgaacaactc gtcggcagcg acatcgtgaa tgccctgctc acgggccctg
    10801 agcatcttga accacttgcc catttccgac ttgaccgtcc gggtatctgc acgatccacc
    10861 gcctctttca cggtctgcaa ccgttggctg tagggttccc aattcgaacc gggatagctg
    10921 gtcttgtaga agttgaggga gttattgatt tcatcgaccc aggactgctg ggcagacaca
    10981 gaccctgcca gcaatgccaa gccgacgcac aacccgaaaa ccaacgcaca gattccatat
    11041 cctctgttct gaatagtcat gatgcgcctc ctctcaatga cacgatccca cccggatcag
    11101 aaggagaccg acgattggcg gtccgactcg cgcgcattct ccgcatcggt ggcgtccggc
    11161 gtcttcagct tattcgtgtc catgcttccg gatgcgccgc tctccggaac catcatgtga
    11221 cgatcaaacc ccttctccat gtcgtttacc gacggctcgg aggatgtcgg actctccccg
    11281 gaaccgatct ccgtttgcgg cgagggaact ccgatcatct ggtcgggatg gatcacttgc
    11341 gcctgtcccc cggacgcaat gaacagcccc aggcccaacc caccgacaat gacgcacaca
    11401 gacttgatac gtgatcccgt ttccatgatg tatccctcct ccatgaagga tgaccgcatt
    11461 gcacaggcac tctgcggcag cacttagaac agtactggaa gcgggacacg gggcctattg
    11521 cctggagggt ggattttccc tccacccttt gagtgaggac ttggagggaa tgaacgggga
    11581 tctagcggct ctgcagatag gtgagtacgg cttcaacccg gccgctcaca cggagcttct
    11641 gataaatatg gtgcagatgg gtcttgacgg tatccaggga cacgcacaat tgatcggcga
    11701 tttctttgtt gctccgtccg gcagccgcgc aggcgagaat ttcatgctcg cgctccgtaa
    11761 ggagcgacaa cccgccgccc tccgattcgc cctgtttgtt caccagggcc atcgccttct
    11821 tggcgaagcc gtcgggcatg aagggagatc gcaccacgtc gccctcgaac gtggcgcgaa
    11881 tgatccgcag aaactcctgg tggtcggcgt ctttgaggat gtacccgcag gctcccgctt
    11941 gcacggcggc cacgatccgg gcatcgtcgt catgtaccga cagcatcaac accttgctgt
    12001 ccggcacgca gctgcggatc aatcgcgtgg cggtgatgcc gtccaatctg ggcatgtcca
    12061 catccatgag gacgatgccc ggcttgtgtg ccaccgcgag atcaaacgcc tgccggccat
    12121 ccatcgcttc cccgaccaca acgagatctc gctcatgttc cagcagcagg cgcaagctct
    12181 gccggaacaa ccgttgatct tcggcaatca cgatggtgat gggcatacct gctcctgcct
    12241 gaccggcaac gcgaccgtaa agaccgcgcc gccttcggga cgattcgccg ccttgatctg
    12301 cccgccatgg gcgtccacca ccaaccggca gaagtgcagc cccaggccgc gtccgatccg
    12361 gtaatcctgc ccgtcttttt ttctgaaaaa catctcgaag agatgcggca ggtcctccgg
    12421 cgcaattcca ggcccctcat cttggatctg aatcgtcacg gtcgaacacg catccggccc
    12481 ggcgacagtt tctccctgtg agacctgtcc cccgttcacc tgcacgctaa tcatgatggc
    12541 gccccgtgcc ggcgaatact tcagcgcgtt gtggatcaaa ttgatcaaca cccgttgcaa
    12601 gcgtcggccg tcccccatga tccacaccgc ctccgaggga acctgcacat tgaaacgcac
    12661 ctcttgcgcc tcggcctcgg agcgaaacag tcgtaacact tcgtgcatca gcagacttgg
    12721 cgacaccggc gacgtactca acggcagtcc cgaatagttc tcctgataga catccagcat
    12781 gtcgttgatc atgcccagca ggagatcagt ggtgaagcgc aggtcctcca gacagcgacg
    12841 cggcggatcc tgccccatct ccggacagcc tccaagccac tccagcgtct tcttgatccc
    12901 ggccagcgga tttctgatgt cgtgggcaaa catcgaggca aactggctta acgacgcgag
    12961 ccgctcggac cgaacgactt gccgctcaag cgccttctgc tcggtgaggt cgcggagttg
    13021 gatcatgatc gattcaatgc ggcccgcttt cttgaccggc acccaatcaa cctccatcgt
    13081 caggtcaccg gtgggtatcg ggacgtgcat ggccccaccc gacaggtccg ccccgctccg
    13141 gatcactgac tccaactgct ccagcagacg aggccgctcc gactcagcga cgaggtccgt
    13201 gaacggccgc cgggcgaggg tcgaggccgg ccgcccgagt gtgacctccc ctcgccgatt
    13261 gatgtccagg atggaaccga acagatcgat cagaattttg gtatcgaccg aatgattcca
    13321 caggagccgg taatgctcct ccgacgtggc gagatcaaga ttcacctgct ccaaccggcg
    13381 gacgtactgc gccttcactc gggatgcctg ttccaatcgt tgcgcgagat cgtcgaaact
    13441 cgcggccagc gcatcgatct cgacaatccc gctgggccgg gaaaaggcct gggacggcag
    13501 cggttccgac ggccatgcgc cgccgaagac attgacccgc tgaatcaaat cgccgagggg
    13561 acgcacgatg cgccgggcca gcctccagcg cagccacacg atgaacacga cggccccgac
    13621 ccagaacgcc cccagtttcc ccatgagagc cagcaacggc tcgaacgtct ccgacggatc
    13681 ctgctgcatc aggatggacc agccacgggc ctgctcgatc tgcgcaggca ggacgacccg
    13741 ggccagaccg atgatgctct ggttcccgcc atgcgtatcc tgctccaact gaaaccatcg
    13801 agcagcggga gcgtccgctg gtgcctgcat agattgtgtc tggagggtcg cgtgaagatt
    13861 gggggcaagc atggaacagg cgagcactac cccgtcctcg gccagcagca tcatgtgtcc
    13921 tgtgcggccg atgcgacttc ccacaatcga ggacaggata cggtccgtcc cgatcacggc
    13981 cttcagggcc cctcgcaccg gctgcccctg tccgccgatc ggcactgcaa cttcccagaa
    14041 accccgcccg ttgtcgtcaa cagtcagtgg accggcccag gcgcgttgag ccgagaacac
    14101 aaccggccac catggttgct gcgcataaaa agtccgtgcg gattcgctct gccggcctcc
    14161 gaccaattgg ccatcccatc ggacaatcac cagcgagtga aaataacgat gctgctcctc
    14221 tcgccactgc tcgaactgca gcgtcggctg cttcgcagag gacacccctt ccaccgaggc
    14281 tcgaatctca ggaagggcac tgagccgttg catccattcg agttccttgg caagcaacag
    14341 ggccgctttg tccgcgctct ggcgggctat ttcctggaag gtaatgccga ctgtggtgtg
    14401 aagtgactgc ttggcatgcc agtaggcata cacgaggccg actgtgccgg aaaggatgcc
    14461 taccgatagc acagagaggg taatcaaact ctggagactg acccgctgac gcatggcggc
    14521 aggccattgg agggagcgac tcgaccggct ccttggacca ggcagcgagt tctgccggga
    14581 gggaatgcca gccggaccgc acatcggtta cgtcatgtca cactccactg ctttcgggcg
    14641 tacactcgcc accccttcag catacaaaat ttcggttgcc gaacataggc ctcacaatgc
    14701 acggtcatcg caactcgaca atagtgacac catcgcctcc ctctgcgcga tcaccggggc
    14761 gaaaggtcac gacgtaggga gacgccttca gatagtcacg tagtacagcc ctcagtcggc
    14821 cggtcccgtg cccatgaatg atgcgcacga acggactccc gccgaagatg gcccgatcga
    14881 gcccggccac cacgatatcc agtgcgtcgt ccgcggcctg gcctcgtaca tccaacgtct
    14941 cctgaatatc ctcgggggcc aggggctgac tggttcgcct ggacgtcacc gtcgtcgtct
    15001 tacgaggctc gcttcgagcg ggctgagggg gtcgtgcgag gccgaccaga cttcccacgt
    15061 tcgctaaaat ttcgccttcc ccgactttca ggcgcacacg ttttttcccc tgcgggcttt
    15121 cgagcaaggt tcccgtcatg cctaacccga ccacctccac cgcatctccc accgacagct
    15181 gatccacggg aatcggctcc tgcgccgctc gcacttcacg gctgactgct tgctccagtt
    15241 cgataagccg gactttggtt tccttggcct tgagcaactt ttggtcgcgc ttcaggtcgt
    15301 cgagcgtggc ttggaccgca gcccgggcac gctgaaattc ctgggacagc tttttcttca
    15361 gtccctgtcg ctcgtcccgc tcagactcct gcagaaaggc cagcagttcc tgagactcgc
    15421 gcgccgcctg ctcggcactc tgcttggcag ccgttgcggc agcgaggtcc tcgctcatac
    15481 gccgctgttt gtcctgcaaa tcattcagca gggtctccag gagccgatta tcgccctgca
    15541 gccgggcgcg ggcgtcctgc agcaacgact catccatccc gagacgcccg gcaatttcga
    15601 tggccgccga ccctcccggc tgcccctgga tcagccgata ggtcggggag agggtgtcga
    15661 tgttgaactc cacactcgca ttggcgaagc ccggggtctc ttgcgccaac cctttcaaca
    15721 agctgtagtg cgtggtggcg accaccttca cgcccgctgc cgccaaccgg cacagcagag
    15781 cgctcgccaa tgccgcgcct tcagccggat ccgtggacgt gaccggctca tcgagcaata
    15841 ccaatgcccg tcctgggtgt atcggcgagg gcgaagtgcc gacgtcggtc ccttcggaca
    15901 cctccttcag caattgcacc atctgcgtaa tatgggccga aaaactggac agatcccgcg
    15961 ccagatcctg cgcatcgccg atatccgcat agaccgaagg gaaaatcgcc atctccgagt
    16021 cgggcgcaca gggaagatgc agaccgcagc gcaccatcaa tgcgaacaat cccagcagtt
    16081 tcagcgtgac ggtttttccg cccgtgttgg gtccggaaat gatcaggact cgcacggttt
    16141 catcgaacac caggtcattc ggcaccacct gttccttggt caggaccagg aacgggtgcc
    16201 gtgcgcggtt gagcaggata cgcccctgct cattgagacg cggcggaacg gcctgcagcc
    16261 gctggctcag cccggccttc gcggaaatgg catcaagctc gcccagcaac gccacactgc
    16321 cggcaagcgc cggctgatga ggcgccacca tcgccgacag ttcccgcaga attctccgca
    16381 cctcacgttc aacatccaaa tccacgactt tgatggagtt attgagatcc accagctcgc
    16441 gaggctccag aaacactgtg gctccgctcg acgagacgtc atgcacaatg ccggggatcc
    16501 ggccctgcat ctcggccttg atcggaacca cgtagcgccc ttcccgttgc gcaaaatagt
    16561 gctcctgcag cacctcctca taccggcgtg agtgcagaat ctgctcgagg cgatgccgca
    16621 tctgccgctt gagatcattg gcgcgctgca tcaacctgtg cagttcaggg ctggctgatt
    16681 ccctcatcga tccgtcggga tcgatggcgg catttaaggc aatggtcagc cggcgatact
    16741 cctcgaccgg tccaagctgc accaccatcg cacccacggc cggggcatcg gcctgatgac
    16801 gcaggagata ccgctgcacg tcatcgatca atcccaaaac caacgcaatg tccctcaact
    16861 cgtgcgcctc aagacaggca cccttggcga cccggcctaa ccactccgcc acattgggaa
    16921 atcggaggac cggaaaggga tcgaccccgg acctcaatcg aagcatgtcc gacgtttcct
    16981 gctggcgcgt ctgggcctcg gtcaggtcgg tttccaggac cagcgatcga caccgctctg
    17041 tcccgattgc agactgggca taggaggcca gatgctccag cagcttggcc cattccagca
    17101 cgttgcacga ttcggactgt aaggtttcag attgcatcgc accccgactg ccggttgatg
    17161 gttacgaaaa aacagactct acccgaccgc ccgggcacag gcaaggcaag cccggaccgt
    17221 gtgaatgacg accggccact agccaacaac cacactcaat ttcctctttt tgtatgcttg
    17281 acacatagaa agtcggtcag tactatcgcc ccagactcaa tcaattccac ccgtttaatt
    17341 cttgaggatt tagccccatg accacacgca tcggcatcaa tggattcgga cgcatcggtc
    17401 gcaatgttct tcgtgcatcc ctcggagacc ccagcctgga atttgtcgcc atcaatgacc
    17461 tcaccgatgc gaaaacattg gcctatttgc tcaagtacga ctcggtccat ggcacgctcg
    17521 acgcttccgt tgaggccaaa gacgaccagc tcattattga cgggaaggct atcaaggttt
    17581 tagccgtcag ggatccgaag gagcttccct ggaaggccct cggcgttgag atcgtcgtgg
    17641 aatctacggg gcactttacc gaccgtgaag gagcgggaaa acatctctcg gccggggcga
    17701 aaaccgtgat catttcggcg cccgcaaagg accccgatgc cacagtggtc ctcggcgtga
    17761 acgagcaagt gttcgatgcc aaggcacacc atatcgtatc caacgcatcc tgcaccacca
    17821 actgcctggc cccggttgcc aaagtcttgt tggaaaactt cggcatcaaa cacggcgtca
    17881 tgacgacgat tcattcctac acgaacgacc agcaactgct cgatcttccc cacaaggacc
    17941 tccgccgtgc gcgggcggcc ggcatgtcga tgattccgac cagcaccggt gcggccaagg
    18001 cgcttcacct ggtcatcccg caactgaagg ggaaattgga tggactggcc atccgggttc
    18061 ccacccccaa tgtttccctg gtcgacctca cggtcgaaac cgaaaaagat tgcgacgtgg
    18121 cagcagtgaa cgccgctttc aagaaagccg ccgaaggtcc gatgaaaaat gtgctggctt
    18181 attcggatgc gccgatcgtc tcgatcgatc tcaaggacga tccacactcc gcgattgtcg
    18241 atgctccgtt gaccgcagtg atcgacaaac gactcgtcaa ggtcaccgcc tggtacgaca
    18301 acgagtgggg ctattcctgc cgcgtgcggg acctgatcaa atacatcgtc gccaaggggg
    18361 cgtgagccga ctcttgcaca acgggctttt ctgacgtgca gcgcaaatcg gcgtgaatat
    18421 cgcctaggct gcacacacca gacgaggagt gaaggtctgt catgaatatt cgcaaacgaa
    18481 tcatcgagga tgtccagctt cgtgggaaac gcgtgatcat tcgcgccgac tacaacgttc
    18541 cgctcgacga ttcattgcag atcaccgacg acaccagaat tcgatccacg cttcccacca
    18601 tcaatcgtgc cgttgatgag ggagcaaagg tcatcctctg ctcccacctc ggccggccaa
    18661 agggcagatt cgatccgaaa ttcagtctgg cccccgtcgc aaaacgtctc cagcgcctgc
    18721 tcggcaaaga agtgattttt gccccggatt gcatcggagc ggcagtcgaa ggtctcgtcg
    18781 ccaagatgca gcccggcgac gtcatgctcc tggaaaatct ccgtttccat ccggaagaag
    18841 agaagaatga cgacggattc tccaaggccc tggcatccct cgccgatgtg tacatcaatg
    18901 acgccttcgg ggccgctcac cgggcccatg cctccacagt cggcatcaca aaatatattc
    18961 ctgaagccgc cgccggttat ctcctcaaga aagaaattga atatctcgaa ggcgccgtag
    19021 aaaatccggt gcgaccgttc gtcgcaattc tcggcggagc caaagtgtcc ggcaaaatcg
    19081 gcgtgatcga gaacctcgga aaaaaagtcg acaaagtcat catcggcggc ggcatggctt
    19141 ttaccttcct caaagccatg gggcttgaga tcggccaatc gctggttgaa aacgacatgc
    19201 tcgacttcgc caagggcgtg caagaccatg ccttttcaag cggcgtcaaa ttctatctcc
    19261 cggtggactg tgttgtcgcg gccagccgtg aaccgggggc tgaaacaaag atcgttccgg
    19321 ttcaggaaat tcccaaaggc tggtacggac tggatatcgg ccccgcctcg gtcaaactat
    19381 tctcagaagc agttcaggat gccaagacca ttctctggaa cggtcccatg ggcatgttcg
    19441 aagtcgatgc ctttgccaga gggaccctcg cgatggccca ttccgtcgcc aatgcctacg
    19501 ccttgaccat cgtcggggga ggcgaaaccg ccctcgcaat ccatcgggcc ggtgaatccg
    19561 aaagtatctc gttcatttca accggcggtg gagcggcgct ggaattactc gaggggaaaa
    19621 cccttccggg actcgcagcc cttcccaacc gtgcagcata acgacaacct ccaccgtttc
    19681 ctccccgcaa tagaggctcc gtgagaactc gcttcatcgt cggcaactgg aaaatgaata
    19741 agaccgcctc tgaagccggg gcattcgttc atcgtctatc gcaggaattg ccccaccatg
    19801 ccggaatcca gatcggcctg acaccgccat ttacggcgct gcacgccgta cgcgaggcgc
    19861 tgggcccggc ctcggccgtt ctcctcggcg cgcaaaatct ctattgggaa gataaaggag
    19921 cattcaccgg cgaagtgtcc ggggcgatgc tgaaggacat cggttgccag tttgtcctcg
    19981 tcggccattc ggagcggcga cagtatttcg gcgaccagga cagctggacg aataaaaaag
    20041 ttctagccgc ccttcggcat ggccttcaac cgattctgtg cgtgggagaa acactccagg
    20101 atcgtgattc gggacagacc gacctcgtca ttcaacgaca gcttcgcgcc ggacttgccg
    20161 ggaccgaggc acacacgctt ccagccctga cgatcgccta tgagccagtg tgggccattg
    20221 gaactgggcg ggctgcgtca ccggagcagg cggtcccggt ccatcgcctc atccgccgaa
    20281 ccatcggcga actgagcgga acggacatag gaaaccacgt ccgcattcta tatggaggga
    20341 gcgtcacacc gcacaatatc gccgactttc tagcatccga agaaatcgac ggcgcacttg
    20401 tcggcggggc ttgtttagac ccggtctctt ttgctacact cgtcaaagtc gcaatcgggt
    20461 ccaaacccac cacggcctaa agactcaaca tgtatacact gctcatcatc attcatgtta
    20521 tcgtctgcct gctcatgatt ggagcaattc tgctgcaatc cggtaagggc gctgaaatcg
    20581 gtgcggcatt cggtggctcc agccaaaccg tattcggcag ccgagggccg gctaacttcc
    20641 tgagcaagtt caccgtcatc actgcatttg tcttcatgtt cacttcattg tcgttggcca
    20701 tcatcgcgaa ggaccggaat ttcgcatcca cggttatcga cttgaacaag aaacctgctg
    20761 caacaactcc aagttcaacg ccgtcagaaa ccacgacgcc ccctgccgca gaatctcatt
    20821 catccggcga gggttcgcac tagcctcctc gcctcacagc tcctactgca tcacaggtat
    20881 cagccgagca catgacgaaa gcccgttcgc cacagccacg ggctgtagcg aattcgcatg
    20941 ctatcgagat gttcgcgtgg ggaagatact gtgatcgatg agactgctag acgggagtca
    21001 gccactgccg atgagcggaa tcagtcccgc ccaccacatc aaaaaatgct ttttgtaagg
    21061 tttgcgtgat cggccctggc tttccgctgc cgatgcgccg ctgatcgatt tccgtcacag
    21121 gggtgagttc tgccgcagtc ccggtgacaa agacttcttc cgccacatac atttcatcgc
    21181 gcgtgaaccg ctcctcgacc acgggaatat tacgctcctt cgccaattgg atgatggagt
    21241 tgcgcgtaat tccctcaaga atggaggtca atggagtcgt ttttaagaca ccacgtcgca
    21301 cgatgaacac attttctccc gtgccctccg ctacataccc ttcaggatcc agcatgatcg
    21361 cttcgtcata gccatcagcc ttcacttccc gtttggccag aatggagttg acgtaatagc
    21421 cggaaatttt cccgcgcgtc atggaaacat tcacatggtg acgtgtaaag gacgacactc
    21481 tggctcgaat ccccttggac agggcctcat cgcccagata ggtcccccat ttccaagctg
    21541 cgatgctcac cttgattgga ttctcagcag ggtagagccc catggcacca tacccgatgt
    21601 acaccaacgg acgaatgtaa caagcctcca gccgattgac ccggacggtt tcgacaatcg
    21661 cttccgagat ttgcttcctg tcgaaaggca tttcgatcaa cccgatgtgg gcggattcaa
    21721 agagacgatc cacgtgctca ggtaacctga aaatagctga cccgctcttc cccttgtagc
    21781 agcgaatacc ctcaaacgct gctaacccgt agtgaagcga gtgcgtgagc acatgaacct
    21841 gcgcatcagc ccaggccaca aacttcccat ccatccatat tttttcgctg gtttgtaaca
    21901 tgcgcgggat aataccagcg cctcgcgacg accgtcaaga caaccgcacg tagacatttc
    21961 gatgcagagt cttcaggaat tccgccgctc ccgccctcct tcacgcagcg gtgcctgaag
    22021 ccagcttcgt tcaatgacgc actaaatctt gacaccttct ccgccgctat gataaacacc
    22081 cacgttctgc tgacgaggag aaagacgcac atgtacgcta ttatcgagac aggcggaaaa
    22141 cagtatcgag tggaaaccgg aaccgtactc caggtggagt cacttccggg tgatgtcggg
    22201 cactctgtgc agatcgacaa ggtcaagctg ctgcatggcg agggcggact ggtagtcggc
    22261 cagccggtgg tggcgggggc gaccgttaca gcggaaatca tccgccaggg tcgcaccaga
    22321 tcgatcacgg tcttcaagaa gcaccgtcgc aagaattatc gccgtaccag aggccatcga
    22381 cagggcttta cgcagctgcg ggtcactggg atcgaaacga agtaacgact cgaaggggag
    22441 tttgccatgg caacaaacaa aggcggcggt tcgacaagga acggccgtga cagtaaccca
    22501 caatacctcg gtgtcaaagc ctacggcgga gagacgatca aggcaggctc catcattgtg
    22561 cgccagc
//
LOCUS       DAORRQ010000066        21941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_144_length_21941_cov_138.761400, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000066 DAORRQ010000000
VERSION     DAORRQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21941
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144_length_21941_cov_138.761400"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..694)
                     /locus_tag="PK224_16645"
     CDS             complement(47..694)
                     /locus_tag="PK224_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17426.1"
                     /translation="MMGMIYRRKKRDPTTGKLEEQGPYWMKYYIEGRPIQESTKVFEK
                     EQAKKILKVKEGEVAAGLYRGPSIDRIRFEDLAALVQQDYQINRRKTVRRITEYQKHL
                     EPYFRKARVSSITTDRIKAYIIKRQEQGAANGTINREIGFLKRMFRLGYQQTPQLVAR
                     VPHIPQLKEHNIRSGFFEHEDFLALRGALPDYAQVPATLAYYSGMRMGEVYSLCQ"
     gene            complement(939..1238)
                     /locus_tag="PK224_16650"
     CDS             complement(939..1238)
                     /locus_tag="PK224_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17427.1"
                     /translation="MSPKPKNTPHRVLLSLPTDLYNTISRVSTAMEKPRAEIIRELLS
                     EQQPILEHMATMLEQAKAGKLDQAMKGWQKMTGEALKGLGVLMSPPDIKRAKPKA"
     gene            1633..2760
                     /locus_tag="PK224_16655"
     CDS             1633..2760
                     /locus_tag="PK224_16655"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRB17428.1"
                     /translation="MNSKSASSAPSTLCWCGSDKPYRDCHLDRDKQTPLQWHEIANEF
                     KRQFSKKFCMHPEMSPSVCSRTIVEAHSVSRSSNLNNIAEDGHVMQFSFSLKHMIPNM
                     GRLTAQRIGVRLASTFTGFCHHHDSSTFAPIDQPIASLSGAHIFLLAYRAICREFFAK
                     KAEMELTRTARALDKGKDKCAQVAIQEFNHYRALGVQAGLSDLIAKKGEYDRALVGRN
                     YDAISHYVIEFDHALPMACTIGFTPEVDFNGNRLQTLHAGTKHADLLTCSITKTTQGS
                     AIIFAWLKEPNGACTRLINSLDELKLHLLPSAIVRLIFEHGENVFFSQAWWNGLAEDE
                     QRIIENRANSFHHKPINCLADDGRRPVLWSIVSRKKVIRSH"
     gene            complement(2820..3425)
                     /locus_tag="PK224_16660"
     CDS             complement(2820..3425)
                     /locus_tag="PK224_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17429.1"
                     /translation="MSLNGIVEISAAILASLGSAGAIVIGCATWLGKVWANRILESDR
                     LKYSEELERLRSDLGKTVHVHRIQFDTEFKVLSDIWGKLSILRSAMGSLRPTMDIVNA
                     GETPEDRLNRRLLRYDQALRVFIEAVDGQSPFYPEDIFQELSIALQIANRESISVAVE
                     KPERSSDWYKEGRQNFQAFTDSADKISALMRKRFANLQVQP"
     gene            complement(3543..4937)
                     /locus_tag="PK224_16665"
     CDS             complement(3543..4937)
                     /locus_tag="PK224_16665"
                     /inference="COORDINATES: protein motif:HMM:NF037531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17430.1"
                     /translation="MRISEPIEKSGYFWLPNNPDTKLPGTLRISEFGKTTLETIGIFG
                     NTIRALIQNSAPKRIVGFLEDGNPITLDRCSPGSKIIRFGNGISKFTLSAGLTLDGAH
                     YASEEEITFTKFKFSVEGLDEWLSISGLQVEENLQDNSARVHFTLPKRISIQLPDGIT
                     LTLTFAGGVEGTSWSTQAKVAQKAYILLTSEEPKSIQDFIALVIKINNFLCFATDKTV
                     SLDSATGYSNDISKESVDGQRHDIPIKLYYQSSPTSKTKPNVQWHRMIFNYSHVATDF
                     EKVLTHWLTNYEISEPAFNLYFASKSGAHKYIDGRFLSLAQGIETLHRRNSKETSMPE
                     EEFNKLIETTLKSCPSDKREWMGKRLKYANELSLRQRIKQMIKPFQHLYGTAMDLKSF
                     TAQVIDTRNYLTHYDTTLSSRATSEIELWNLCMKLECLFQLHFLQLMGLEIESINNLV
                     KDNYAFRKKLETSV"
     gene            complement(5119..5793)
                     /locus_tag="PK224_16670"
     CDS             complement(5119..5793)
                     /locus_tag="PK224_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17431.1"
                     /translation="MNEARARGRAKGTIRKKCLTIGADHLVTLTYRANVEDRDRVLHD
                     LERLRRALSRSGCSMPYVAVLERQQRGALHPHLAVKGFQDVRLLRRCWYKIVGNGQGQ
                     VNVRGPRPGSSPVKLARYLSKYISKDFDNMPREFEEHRYFCSLGVKVPTEKHEFVLDR
                     RAKDVERKMYSIIRQEALRRVGVCCRLTEWMGGAGTYGWIAGFEDSSIRWVNVKTEPV
                     PITPSS"
     gene            6145..6366
                     /locus_tag="PK224_16675"
     CDS             6145..6366
                     /locus_tag="PK224_16675"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRB17432.1"
                     /translation="MDDRIVVDSKVCSGKPTIRGTRIMVTNILGMVAGGYTVARIIEA
                     YPELTNDDVTAALEYASQVIDEEKVIPRA"
     gene            6359..6739
                     /locus_tag="PK224_16680"
     CDS             6359..6739
                     /locus_tag="PK224_16680"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRB17433.1"
                     /translation="MPDRLTILLDQNVPQAVVSWLKLLKPTWAVFHTGDVGLSGKADE
                     EVFVWAQQQAAVIVTFDEDFADGRSFPAQRHHGIVRLRVWPTTIEETQQALERLVIEV
                     TEAELTHSLIIVDRSRIRVRTRPQ"
     gene            complement(7150..7467)
                     /locus_tag="PK224_16685"
     CDS             complement(7150..7467)
                     /locus_tag="PK224_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17434.1"
                     /translation="MKLNVLTFMLILVLLPELSYGRCRTVGESASLGTAYGAFPLYPA
                     KRVIANGEKRDNVALQWVGYGIGLPLFVVAMPFAMVGAGMGAALHPWTKCDQHDDDWP
                     GLK"
     gene            complement(8392..8526)
                     /locus_tag="PK224_16690"
     CDS             complement(8392..8526)
                     /locus_tag="PK224_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17435.1"
                     /translation="MSAHKSFSELSLKNHQYKKLALASAILFVAVIAILVLELIGAKH
                     "
     gene            complement(8567..8893)
                     /locus_tag="PK224_16695"
     CDS             complement(8567..8893)
                     /locus_tag="PK224_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17436.1"
                     /translation="MKATQTELEHLEMASTTILSQANDRLRVLSLSLQEAGQFERGAL
                     VRALHAEIRHTLPLITLHLNELGRQPERPDAPPHAQQVAESFYILDRALRQLRALASD
                     VRNYNA"
     gene            complement(9046..9696)
                     /locus_tag="PK224_16700"
     CDS             complement(9046..9696)
                     /locus_tag="PK224_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17437.1"
                     /translation="MNNIRVLLVEDHTLVRAGFRVLLEKIEGILVIGEVSDGRSATKL
                     AKELDPDVVLMDIAMAGMNGLEATRRIRQESPRTKILMLSMYTNEEYLKEALRAGAAG
                     YLLKDADRNELELAIKAVWRGETYLTPSMTKFAVDAYCQQGEAQTGPLARLTGRQREI
                     LQLIAEGCSTKQIAQRLDLSVKTVETHRAQLMERLDIHDVPGLVRLAIRTGLVQSD"
     gene            complement(9689..11224)
                     /locus_tag="PK224_16705"
     CDS             complement(9689..11224)
                     /locus_tag="PK224_16705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB17438.1"
                     /translation="MGEGHRNRRQTLAILSTSPTGIITSFNEASERLLGYSAEEVVGA
                     LPLAAFHDPSELQQRAAACTSKPETRATDIFRIIVANAEPGHTTEHDWTYLHRNGRPL
                     PVHLSISAFPDGSGHIAGYILVLQEIREEIRSNDVLQHHAKLLDLANDAILVRDLERD
                     TIDYWNDGAVRLYGWTSKEAAGAYIHDFLKTTFPQPLADIKKTFLAKGYWAGELLHTT
                     RNGQRITVSSRWTLLKTSDGAASASLELNTDITEQKRAQEALKAAHEELEHRVAERTA
                     ALSDANSRLRILSRRLMEIQELERRAIARDLHDEIGQALTAIKMNLRELGELRDGSTA
                     AAGGKPLSDSLQILDQVLRHIRNLALDLRPSMLDELGLVPALRWYIGRQAERAGWKVQ
                     FLADEVMTRPSPEVEISCFRLTQEALTNVARHAKATVVEVRLEMGRRQLDLIIRDNGI
                     GFDSNLIRTGARAGTSVGLSGMEERVRLAGGHMVLQSAPSTGTEIRATFPIGPESAET
                     DHE"
     gene            complement(11515..14832)
                     /locus_tag="PK224_16710"
     CDS             complement(11515..14832)
                     /locus_tag="PK224_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17439.1"
                     /translation="MGFLRDGNTARALPYPEPEFPDKDTEVSPSPVQEQPHHTARLEP
                     TDWVVAAVLTLSVSIACFFIAESIPAFLLQSMDFWFESDTVREVSNMTSTTDDHSRTS
                     VHPLFSILSFVPVYLVQHALAIPPLQAVLYVTSILGGLWMGTLYLLLRLLGCRTLDAS
                     VFTSLGLSSASAIFWLPVPNSYTWGSWSVMISLALLLLAEQHRVGALSYVLASAFTLS
                     ITVTNWMAGLLTTLARWPFKQAVQLSINAFCLVVLLWGAQKFIFPSAEFFIGSRKEAN
                     WINHPQSGQASNIASSFAFHTLIAPAIRFIDDDGYIQVGDDSVRLAQRLAFQFSTPGS
                     AGPLGLIAVCLWSALLLNGLWRLVTMDRQLRFRLVLATLLLFELSLHMVYGEETFTYS
                     LNFTPLLIALAALGTLSPGRPAVLVLAGLLALCAGINNWQQFRQATESATHFTPQRDA
                     MNSLMQNDPDRPWPRSVGHIPLAVPGAPEAEHAYHEPGGDFSPQAPSFGVSLWLCDAE
                     GRPLVTSQSVPLNDIQQTFEPSTHPHVPAIATRTPYYDATWTRLDATHWELRFKNHTS
                     HTPAIMIRSVGPAGGPVTELGWDGEQLDINHRWTVRVTPAPAKVSLGDEQTVKGMTSE
                     TENRSWANASGWGYARLALPREVSAAGDEYRLILSDLRIPIQMQRWYRNAPEQIHLDF
                     PDKRFETSMAAQTTHLMMSLIGRETRPGDPTFFYRAWQRQGAYIATALARAGDPHVSR
                     VLAQYLATHDFAGGNGPEADAPGLTIWALTTSADYIADHEHDQWLWPHVLRKAQRIEA
                     MLTARAPIEESFAVPSPYDLQHGQQIRRSVLAKPSRDGLIVGRVGDEWPSLYVNAVSY
                     RGLLAAAEFAERLGKHRYATGWRNHAHTLRDNWQRRNRNGASDAKISTVSLGPSASIT
                     SNREQLGQALTVYRPSPDTATVATQLSDAHLALRQGRPDAVWAALHRLWTQQASPGLY
                     TWDMPRPATTDVADGWQYVRGWRNQSVVSPDYETAALLLLLQQDMLAYVDDGAAEPTV
                     VIGAGVPQAWISHSMTVSNLALPGGSIDWRWDGHAMRVTLRGPSRKIQLGASFPDDTD
                     MSVTHDNRAADVTARVS"
     gene            15202..16563
                     /locus_tag="PK224_16715"
     CDS             15202..16563
                     /locus_tag="PK224_16715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HRB17440.1"
                     /translation="MHQYAIFYGAAALAVVVWWLTFRDGSARAPFLAILGAVLGGLVL
                     WAWQVSEPSVLFSDFNVAYYPAGRAILEDIPHLFVRCWDTPVCGFVNIPIVAFLFTPF
                     SFLTLQHAQWLLMGLSAVSLVMSLALLWSLTDRTRSRGWAMLLLFALNGPLVYSLKEG
                     NLTHFALLLLIAGVVCLDKTWDRSAGSCFALAAIIKLPLLLFAVYFFGKRRWAAVFGY
                     GLTLLAVTGLSIWYAGWASHVAWYREVILPLSDKGIAAFNVQSVQGLLLRLQDGVRLY
                     DWKPVAVPWDVGAAGQACAALFVGLSGWLFLRSPGLQVRETTNLELSMVLCLSLIISP
                     ISWTHYYLLLLLPLSLYVGNRLPVATHGGWAVGMTLCLLLISPPVTFPGPDQVAGDQI
                     ATLFLSHYVIGAILLWGLCGYARWSVANVSRLRLVAAGSGPPVPVRADLPQESDESHT
                     RSA"
     gene            complement(16685..17287)
                     /locus_tag="PK224_16720"
     CDS             complement(16685..17287)
                     /locus_tag="PK224_16720"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HRB17441.1"
                     /translation="MSRLRPGSRLLTMLMAGLLAIGCWQLGEGSWIYAKAGLAQFLLQ
                     RAWCRALAGEAMPKPWPWADTWPVARLRMQHPSVDLIVLAGAYGRTLAFGPGHVTSSA
                     LPGQEGTVMLTGHRDTHFRFLKDVKLDDHIALTGSDGTTLQYRVIEQRVMDSRLELIP
                     TGQDTEDLVLVTCFPFDAIRAGGPLRYVVRAERVLDRGHS"
     gene            complement(17284..19422)
                     /locus_tag="PK224_16725"
     CDS             complement(17284..19422)
                     /locus_tag="PK224_16725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HRB17442.1"
                     /translation="MLTDRFGSLRQRAKTSAARLMLCAFFLSLALGAPLPATSCAEPD
                     ETLNTGIVSTMGINDVTEGTLMFRTDQAGRFTPAPVLKTDVHIAITGTIARATVRQEF
                     TNPSKKKGDWLEGVYVFPLPETAAVDHLRIKIGERIIEGRIKERAEAKQLYDQAKREG
                     KRTGLVEQERPNLFTTSVANIGPGEHVTVELEYQDAVRYENEEFQLRFPMAVGPRYIP
                     GVPVVVEGQDLQGSGTSPDTDRVPDASRITPPIHPPEDGAINPLSLSLSLNPGFPLAK
                     VESPFHPIITIQDQGGGYQIGLREDAVPADRDFQLIWHPAPRTEPMATVFTEQKDGAT
                     YALLMLVPPTQHHEKAARVPRDITFIIDRSGSMAGASIEQAKGSLAAALARLTTQDRF
                     NIIQFNHTVRSLFSIPQPVTTTTMQQAIRYTEQLTADGGTEILPALRQALKSPQDSAR
                     LQQIILITDGQVGNEEELFELLHQRVGSRRLFTIGIGSTPNSHLMRKAAETGRGTFTY
                     IGNVNEVKDKLDGLFKKLEHPVLNDITIDAAGWSGLEQFPATITDLYEGEPIVLTLKA
                     DSLPSQSVLRGQIGRAAWSLPISFNDAPTHGGLSVYWARKKIAALMDETYKGGAEETI
                     RKAVLDVALAHHLVSRYTSLVAVDVTPARPTDSPVTERDQTSNLARAQGLTALASLPR
                     TATGAQLQILLGAAALMLASLLWQFRRAVA"
     gene            complement(19520..20332)
                     /locus_tag="PK224_16730"
     CDS             complement(19520..20332)
                     /locus_tag="PK224_16730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRB17443.1"
                     /translation="MQKAPLASVNAGPVSPTVMGPEKEMKDVYIMSGLVFFLAVVVSA
                     FWYYSQADEAAHSNPSEEALNSAQVAQVLKDPTEHAPVSASIPRTLVDGTPLATRSTD
                     ILHDDIHFEIGRKGLTDDGKAALQRHAEFLTSERDWGILLQGYTDQQGSRSFNKILGM
                     KRAETVKQQLIALGVPESSIRTVSLGEEGALCIDNSDVCRRMNRRVHVEMRRIGQEHM
                     IVPITATETVTDPFSNSVDPSIETGGSGASSEGLPLTTSEPAERTSEPTDGN"
     gene            20867..21679
                     /locus_tag="PK224_16735"
     CDS             20867..21679
                     /locus_tag="PK224_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17444.1"
                     /translation="MKKSLLLLLLLWAAMFSLAQAGDYQLIIGEQVDVCEACKRNLER
                     MTVHPACERDYSDQLGLEAPEWKPFDVGGHIGAMERVLRYLATGDEFADDRYNSDDDR
                     YADPVRRIGRKEEPWAYWAYVDINNDGKAEPVLKLHSALCKLRNGGSIGQAYSAPIVV
                     LNENLSGIDRALTDLVVQNPRKEGTVAGTTNYQLYGVFSFKGEMYFDRWNDTETGDER
                     ESFTLSIYEAKGDTTRTLCQMKEVQVRRWSPGKPCAFRHCPPAKPFPVNLTP"
BASE COUNT         4482 a         5917 c         6446 g         5096 t
ORIGIN      
        1 ggggaggggt ccatttcaca tgatgacagg ggtcaatttg gcatgattac tgacagagtg
       61 agtacacttc tcccattctc atgccactgt agtaggccag cgtagccgga acttgggcat
      121 agtcggggag ggcaccgcgc agggcgagga aatcctcatg ctcgaaaaag ccggagcgta
      181 tgttgtgctc tttgagctgg ggaatgtggg gcacgcgagc tacgagctgg ggcgtctgtt
      241 ggtagcctaa tcggaacatc cgcttgagaa atccgatttc ccggttgatc gttccgttag
      301 ccgccccttg ctcctgtcgt ttgatgatgt aggctttgat ccggtcggta gtaatggaag
      361 acactcgggc tttccggaaa tacggttcca agtgtttctg atactctgtg atccgtcgaa
      421 ccgtttttcg ccggttgatc tggtagtcct gctggaccaa tgccgccagg tcttcaaatc
      481 tgattcgatc gatactcggc cctcgataca gtccagccgc gacttctccc tctttgacct
      541 tgagtatttt cttggcttgt tctttctcaa aaaccttcgt gctttcctga atcggtcggc
      601 cctcgatgta atacttcatc cagtaggggc cttgttcctc tagtttgccc gtggtcggat
      661 cacgcttctt gcggcgataa atcatgccca tcatggacct ccgttttgtg ggtttcgatg
      721 tgccgctcca aatcatgaat atcgaacagt actcggcgtc cctgccgtac acaaggcaga
      781 gtccgcaatt tctgaagatt ccgaaccgac caagggctga cacccaggta gcgagccgcc
      841 tccctcacag aatacagtcg tttaagcact tgctctgaca tctttttcac ctccttccat
      901 ctcgaaatga ttgtattgca tagaaaagtt ggcccctctt aggccttggg cttcgccctt
      961 ttgatatccg ggggagacat gagcacgccc agacctttca atgcctcccc ggtcatcttc
     1021 tgccagccct tcatggcctg gtctagtttg cctgctttcg cctgctccaa catcgtcgcc
     1081 atgtgttcga gaatcggttg ttgttcggat aataattcac ggatgatttc ggctcgcggc
     1141 ttttccatgg cagtggatac acgcgaaatg gtgttgtaga ggtctgtggg taaagacaac
     1201 agtactcgat gcggtgtgtt tttaggcttt ggactcattg atagccctcc gttatacctc
     1261 tatatatcgt ttcgatatac agcagtcaag taggacacaa ccttagaaaa ccagccattg
     1321 cactcccaag attcagacag ctcatcgagc actaatcaca ttgcgacaag aattgatcaa
     1381 tgaccaaaaa aataatagag ttgcgtggtt tgtcgcgagt ggatggatca acttagttgc
     1441 gaatggcaga gggtgatgga gagtcagcca gtcggttggt tagggtatcg gcaagtcggc
     1501 tgatgccgtt atgaaagact tgtggcaccg tacagcagta ctcttcaaga ccacccacat
     1561 aatgaagagc ttcagcgcga gtcgaccatc tcttgtctga tgaagaacgc ttccataagc
     1621 gagtaaagga caatgaacag caagagcgcc tcctctgcac ccagcacact ttgctggtgt
     1681 ggctctgata agccctaccg agactgtcat ttagatcggg acaaacagac gcccttgcaa
     1741 tggcatgaaa ttgcaaacga gtttaagagg cagttttcaa agaaattctg tatgcatccg
     1801 gaaatgtctc cctcagtatg ctcacggaca atcgtagaag ctcacagtgt ctcacgatca
     1861 tcaaacctga ataatattgc ggaagacggc catgtgatgc agttcagttt ttcgctaaaa
     1921 cacatgattc cgaatatggg gcgactcacc gcccaacgga tcggcgtgag attggcttcc
     1981 acgtttaccg gattttgcca ccatcacgat tcttccacat ttgctccaat agaccagcct
     2041 atcgcgtcct tatcaggcgc acacatattc ttgttagcct atcgggcaat ttgcagagag
     2101 ttcttcgcaa aaaaggcaga aatggaatta acaagaacgg caagagcgtt agataaagga
     2161 aaggacaagt gtgctcaggt tgcaatccag gagtttaatc actacagggc gctaggcgtt
     2221 caggcgggcc tatctgattt gatagcaaaa aaaggcgagt atgatcgcgc actggtgggc
     2281 agaaattatg atgcgatctc gcattatgtc atagagttcg accacgcgct tccaatggct
     2341 tgcaccattg ggtttacccc ggaggtggat tttaacggga atagacttca aactctccat
     2401 gctgggacaa agcacgcaga cctattaact tgttcgatca cgaagacaac acaagggagc
     2461 gcaattatat ttgcctggct taaggaaccg aatggagcat gtactagatt gattaacagc
     2521 ctggatgaat tgaagcttca cctgctcccc agtgcaatag taaggctaat tttcgagcat
     2581 ggtgaaaatg tcttcttttc ccaagcatgg tggaatggct tggccgagga cgagcaacga
     2641 ataattgaaa atcgcgcgaa ctcctttcac cataaaccaa ttaattgtct tgccgatgat
     2701 ggccgtaggc cggtcctatg gtcaatagtt agtaggaaga aagtgattcg atcgcattga
     2761 aaatcacatc cacaaaccgt aaccgtttcc aggtccaccg attacctatg aagttttgtt
     2821 cagggttgta cttggagatt ggcaaaacgc tttctcataa gtgcagaaat tttatcggca
     2881 gaatccgtga aggcttggaa gttttgacgt ccttccttgt accaatcaga actcctctcg
     2941 ggtttttcta ctgcaacact tatgctttct cggttagcaa tttgaagcgc tatcgatagc
     3001 tcttggaata tgtcctccgg atagaaagga ctttggccat caaccgcctc aatgaacaca
     3061 cgcaacgctt ggtcatacct caggaggcga cgattcaatc tatcttccgg cgtctcaccc
     3121 gcgtttacaa tgtccatagt aggccgtaaa gatcccatgg cggaccgtaa aatggacaat
     3181 ttgccccata tgtctgataa aaccttaaat tcagtgtcaa attgaatgcg atgaacatgt
     3241 accgtctttc caaggtcgct tcggagtcgt tcgagctctt cactgtactt gagacgatct
     3301 gactccagaa ttcgatttgc ccagactttt ccgagccaag tagcacaacc gatcacaatg
     3361 gcgccagcac tcccgaggct tgccagaata gcggctgaaa tttcaacaat tccattgagg
     3421 ctcatcttcg tatttcctct ggcattccat ggaataccct cacacaaaag ctagtgatta
     3481 gactgttgga gtaagtctct ttcgaacgcc catccccgct gaatcgaagg ccatagttgg
     3541 ctttaaacac tcgtttcgag ctttttccgg aaagcatagt tgtccttaac gagattgttg
     3601 atagattcga tttcaaggcc catgagttgc aaaaagtgca gttggaagag gcactcaagc
     3661 ttcatgcata gattccagag ctctatttcg ctagtggctc gacttgaaag cgttgtgtcg
     3721 tagtgagtga ggtagttcct cgtatcaatg acttgagcag taaatgactt taggtccata
     3781 gcggtgccgt acaaatgttg gaacggcttt atcatttgtt ttatccgctg gcgtagtgac
     3841 agttcgtttg catacttgag cctcttgccc atccactcac gtttgtcaga agggcaactt
     3901 tttaaagtag tctcgatcaa tttattaaac tcttcttccg gcatggacgt ttccttggag
     3961 tttcttctgt gcaaagtttc aatgccttga gcgagagata agaaacggcc atcaatgtac
     4021 ttgtgtgcgc cagatttgga tgcaaaatac aaattaaatg ctggttctga aatttcataa
     4081 ttcgtgagcc agtgtgtcaa aactttctcg aagtctgtgg cgacgtggct gtaattaaaa
     4141 atcatccggt gccattgaac atttggcttt gtcttagaag tgggcgaact ctgatagtag
     4201 agctttatcg gaatgtcatg tctttggcca tcgacggatt ctttactaat gtcgttcgag
     4261 tagcctgtgg cagaatcaag ggagacggtt ttatccgttg cgaagcagag gaaattgttt
     4321 atcttgataa cgagcgcgat gaagtcctgt atagattttg gctcttccga tgtaagtaat
     4381 atatatgctt tttgagctac cttcgcttga gtgctccaac tagttccttc tacgccccca
     4441 gcaaaagtta acgttaatgt gatgccatct ggtaattgaa tcgagattct cttgggaaga
     4501 gtgaaatgta ctctcgcaga gttatcttgc aagttctcct caacctgaag gccagatatt
     4561 gagagccatt catcaagccc ttctacagag aatttaaact tggtgaacgt gatctcttcc
     4621 tcactcgcgt agtgtgcccc gtcaagtgta agtcccgcag aaagggtgaa tttggatatt
     4681 ccattgccga atctgataat tttggatcct ggagagcatc tatccagggt gatgggattg
     4741 ccgtcttcaa gaaatccgac aatacgcttg ggagctgaat tctgaatgag tgccctaatt
     4801 gtgttgccga atatgccaat agtttcaagg gttgttttcc caaactctga aatgcgtagg
     4861 gtgccgggca atttggtgtc cggattgttc ggtaaccaga agtatccgga cttttcgatg
     4921 ggttcagaaa ttctcatcct aaccgtcaaa tcgatggata accatggtca tactttatga
     4981 ttgccgtttt accatttcgg ccttctctaa gcagtaaaat gaataatgtg ctttgtgcat
     5041 ttatgaaggg ggagtctacg acacccccct tccccccgta aagatcgccc gcgcttcggc
     5101 aagcgccggc gcagtgagtc atgaactcgg agtaatcggc actggttcag tctttacgtt
     5161 aacccatcgt atcgagctat cttcaaaccc cgctatccaa ccgtatgtgc ctgctccacc
     5221 catccattcc gtaagacggc agcagacgcc aacgcgacgc agcgcctctt gccggataat
     5281 gctgtacatt ttcctttcaa cgtcctttgc ccttcggtcc agaacaaact catgtttctc
     5341 cgtcggcacc ttgacgccta aggaacaaaa gtatcgatgt tcttcaaact cacgcggcat
     5401 attgtcgaaa tccttactga tgtatttgga caaatagcgg gcgagcttta ccggtgaact
     5461 tcctggacgt ggccctctga cgttcacttg tccctggccg tttcctacga tcttgtacca
     5521 acaccgtcga aggagccgaa catcctgaaa ccctttcacc gcgaggtgtg ggtgcagcgc
     5581 tccacgctgt tgccgttcca gaaccgccac atagggcatg gaacagccgg accgtgagag
     5641 ggcacgccgt agtcgttcga gatcgtgtag gacccggtct cgatcctcga cattcgctcg
     5701 ataggtcaag gtcacgaggt ggtcggctcc gatggtcagg catttcttgc gaatcgtgcc
     5761 tttggcccgg cctcgtgccc gagcctcgtt catctttctg ttccgcgagg tgcctttctc
     5821 tcctttcttg ccctggatcg gatctaacct gccccaagac atttcgaccc attgttttcc
     5881 gaaatcatga atcgtgatgg accagacatt ccctgcggga ggtagtgtga tgtgagggca
     5941 tgtgtgatcg ccgagaaatg tcctcctgtc aagtttagga gtggccgctc gcgcggctcc
     6001 cgtgcgcggg tggcttgcgc cagcccgcgc accggcgtcc tgcttgcggt tcctgctatt
     6061 tggactgagc ggcttatctt gacaggacac gtggaacctc ctagccttat actgacaaac
     6121 cagttagtca gtgaggaact tgtcatggat gatcgtatcg ttgttgattc caaagtctgc
     6181 agcgggaagc ccacaattcg gggcacccgc attatggtga cgaacattct cggcatggtg
     6241 gctggtggct atactgtggc ccggatcatt gaggcgtacc ccgaattgac caacgatgat
     6301 gttaccgccg cgcttgaata tgccagccag gtgattgacg aagagaaagt cattccgcgt
     6361 gcctgaccgc ctgaccattc ttctcgacca aaatgttccg caagccgtcg tttcatggct
     6421 gaagctgttg aagccgacgt gggccgtctt ccacaccggc gatgtgggat tgtctgggaa
     6481 ggctgatgaa gaggtcttcg tctgggcaca gcaacaggca gccgtcatcg tgaccttcga
     6541 tgaagacttc gccgatggcc gttcctttcc cgcacagcgc caccacggca ttgtgcgcct
     6601 tcgggtttgg ccgacgacga tcgaagaaac ccaacaggcc ctggagcggt tggtcattga
     6661 ggtgacagag gccgaattga cccactccct catcatcgta gaccgctctc gaatccgtgt
     6721 tcgaacccga ccccaatagg ctgcacgccc cgcaaaaaac gggacgtgca gtgtaaccaa
     6781 aagtgtaacc gtcgcgaccc tttttcgcgg ttttggtcgc aactccacgc aacacaacac
     6841 aatcacctaa cccaatggtg gatcagggtc ttctgtgtgt tttagtggtg tgaggtgagg
     6901 gggagaaatt gcttcgggac cgcttcgtaa accgcaggtc ggtggttcga ttccactcgc
     6961 cggctccaga agtattgatt caaagctcag tattggcgaa tccagcgtag gcgctgagac
     7021 cgttcaaacg attctctccc ctgagtcaat tcgctgcccc gcctcgcaat atttcccaat
     7081 cgtgatgagt atcgcgctgc atcgatgtgc gtcagattgt gcaggtcgag agctgccaca
     7141 atggttgcct tacttcagtc ctggccaatc atcatcatgc tgatcacatt tcgtccaagg
     7201 atgtagcgcg gcgcccattc ctgctcccac catggcaaag ggcatggcca caacgaagag
     7261 cggcagcccg atcccgtaac cgacccactg cagcgcgaca ttgtcacgtt tctcgccgtt
     7321 ggctatcacg cgttttgcgg ggtagagggg gaaggcgccg tatgctgtgc cgagactggc
     7381 actttcaccc accgttcggc agcgtccgta gctcagttcg ggtagcaaca cgagaataag
     7441 catgaacgtc aggacattca gtttcatatc ggatcacccc cgtcgcacat cgtggatgta
     7501 tgagtcccac gtttgagtga gtggccaatt ccaaggaaag caagatacac accacgtctc
     7561 tgattgcagc tcactcgggc tccacgagca gcatcaattg gtcgtatctt cttctccggc
     7621 gcggttacgt gtcatgtggt tccacgacct gtgctgcagc agggagtttc tcgtccatgc
     7681 aaggtgagcg attctgctcg ctgcgctcac ggtgattccg ctcgggaagc ccccctttcc
     7741 attccgagcg caaaactgcc gctggatgcg agaaggccct gaaccacttc aggaaagatc
     7801 cggacgaacc ggtgatggaa ccggttttgt ttgcggaaca ggaggaagtc gtggtcagag
     7861 gtcacgttat gccggtatgc gccggcccgg tagggatcga tgtcagagac tggcgtggcg
     7921 tgagcgaacg aattgccgat cacgaagacg gtcggcttca acaactgcag aatcccgaac
     7981 ctgtggaaga cctatagaag ggtgggggcg atgcatccgg tgtctcgtcc cgctgagggt
     8041 tgtttccgtc agcacgtccc tattgtgctc tgtggcatac ccgccgaagt tcggacccga
     8101 cgtgatcagg cctgccttgt ttttcatgct ctcaacggat agatggtcat tgctcaaaag
     8161 gtgcacggcc tgtatcacca tttctccgaa gcagaggctg atagtcacaa gtacaagaat
     8221 gacggtgccg gaggcgagga tggtggcagg tcgaatggat gacactggtg cttcgtcact
     8281 gacagatggc ccgcagagag gggtatctga cggacccttg gcgttctggc gtgcccaatt
     8341 gcgtttcatc gggaataccc gtccatgtcg aggcgtgctc gtgcggagcg atcagtgttt
     8401 ggccccgatt agttcgagga cgagaatggc aatgacagca acaaagagaa tggcagatgc
     8461 cagggccagt tttttgtatt ggtggttctt caggctcagt tcactgaagc ttttatgcgc
     8521 agacatgagt cgtgttcctc ggtagcgtct tttgaaagcg agcggatcat gcgttgtagt
     8581 tacgcacatc cgacgccaag gcacggagct gccggagcgc ccggtcgagg atgtagaagc
     8641 tctcggcaac ctgttgtgca tgcggcggag catccggacg ctcaggctgt ctcccaagtt
     8701 cgttgaggtg cagtgtgatc aacggaagag tgtgtcgaat ctcagcgtgt aaagcgcgga
     8761 caagtgcacc ccgttcaaat tgcccggcct cttgcagact gagagagaga actcgtaagc
     8821 gatcgttggc ctgagacagt atcgtcgttg acgccatctc aaggtgctcc agttcggtct
     8881 gtgtagcctt catcgattcc gagtccattg cggctctgct atccctgttg gtcacgtgct
     8941 agccgcgggt agggtgccac aatggaccat acccgcggaa tcagggatac cctggaaatc
     9001 gatccaatta cacctgagga tggggggact tcggcgttgt gggggctagt ccgactggac
     9061 gagaccggtg cgaatggcta gccggactag tcccggcaca tcgtggatat cgagccgctc
     9121 catgagctgc gcgcggtggg tttcgacggt tttcacactt agatcaagac gttgtgcgat
     9181 ctgcttggtc gagcatccct ctgcgatcag ttggaggatt tcacgctggc gaccggtcag
     9241 ccgtgccaga gggcctgtct gcgcttcgcc ttgttgacaa taggcatcga cggcaaactt
     9301 ggtcatgctc ggtgtcaggt aggtctcgcc ccgccatacg gctttgatgg ctaattccag
     9361 ttcattgcga tccgcgtctt tgagcaaata accggcggcg cctgcgcgaa gcgcctcttt
     9421 gagatactcc tcgttcgtat acatagagag catcaaaatc ttggtccttg gagactcctg
     9481 tcgaatgcgg cgggtcgctt cgagcccgtt catgcccgcc atggcaatat ccatgagcac
     9541 gacatcggga tccagttctt tggcgagctt cgtggcgctt cgaccgtcgc tgacctctcc
     9601 gatcacgagg atcccctcga ttttttccag caacacccgg aatcctgcgc gtaccagggt
     9661 gtgatcttcg accaggagta ctcgtatgtt attcatggtc tgtctcggcc gactccggac
     9721 ctatcggaaa tgtcgcgcga atttcagttc cggtcgacgg ggctgattgg agcaccatgt
     9781 gtcctcctgc gaggcgcacc cgttcttcca tcccggagag ccccacactc gtccccgcac
     9841 gagcgcccgt gcggatgagg ttcgagtcaa acccgatccc gttgtcgcgg ataatgagat
     9901 cgagttgccg gcgacccatt tcaagacgaa cttccacgac tgttgctttc gcatgccgcg
     9961 cgacattcgt caaggcctcc tgggtgagcc gaaaacagga gatttccact tcgggagagg
    10021 ggcgcgtcat cacctcatcg gccagaaact gaaccttcca tccggcacgt tcggcctgtc
    10081 ttccgatata ccatctcagc gctgggacta ggcccagttc atcaagcatg gagggtctca
    10141 agtccaacgc caggttgcgt atatgccgga gcacttgatc gaggatttgc aggctgtcgg
    10201 aaagtggttt gccgcctgca gcggccgtcg aaccgtcgcg cagctcgcca agctcacgca
    10261 ggttcatttt gatggccgtc aacgcctgtc cgatctcatc gtgcaaatcc cgggcgatcg
    10321 cgcgacgttc caactcttgg atttccatca gccgtctgga gaggatgcgt aagcggctat
    10381 tggcgtcgct caatgccgcc gtgcgttccg cgacacgatg ctccaattct tcatgtgcgg
    10441 ctttcagcgc ttcttgcgcc cgtttttgct cggtaatgtc cgtattcagc tcgagacttg
    10501 cgctggctgc gccatctgag gttttgagca acgtccaccg gctggagacg gtgatcctct
    10561 ggccgtttcg agtcgtgtgc aggagttccc cggcccaata gcccttcgct aaaaaggtct
    10621 ttttgatgtc cgccaatggc tgcggaaacg tggttttcag gaaatcgtgg atataggcgc
    10681 ccgcggcttc cttgctggtc cagccataga ggcgcaccgc cccgtcattc caatagtcga
    10741 tcgtatcgcg ctccaagtcg cggactagaa tcgcatcgtt ggcgaggtcg agcagttttg
    10801 cgtgatgttg gagcacgtcg ttcgaccgta tctcttcgcg gatttcttga agcaccagga
    10861 tataccccgc gatatggcct gatccgtccg ggaatgcgct gatcgagagg tgaaccggaa
    10921 ggggccggcc gttgcggtgc agataggtcc aatcatgctc tgtcgtatgg ccgggttcgg
    10981 cgttcgcgac gatgattcga aatatgtccg tcgccctggt ctccggtttg gatgtgcacg
    11041 cggcagcacg ttgctgcaat tcgcttggat catggaacgc cgcgagggga agtgccccca
    11101 cgacctcttc cgcggaatac cctaagaggc gctcagaggc ctcgttgaag ctcgtaatga
    11161 ttccggtcgg ggaggttgag agtatggcaa gcgtctgccg ccggttgcga tgtccctcgc
    11221 ccatctcgcc tccgttcctt ccgcttccat ttcgggagga atatggtggt tgccagtcaa
    11281 cgggagcctc ttaggctagc attagatttc ttccaattgc tatcgctgtc agatgattgt
    11341 cggcggtgct gcctgctgat aaggctgcat ccgtttgaag gatgccctct gccttcaggc
    11401 cgacatcagg atcgcaatgg gagggcactg aatgaaaaat gatcgagtcg aagcggtgag
    11461 agaccactct gcggctcggg tgatggtcgg ggcgttccct cgaatgccga tggctcagga
    11521 cacgcgtgcg gtgacatcag ctgctcgatt gtcatgtgtg acggacatgt ccgtgtcgtc
    11581 agggaaggag gcgcccaact gaatcttgcg cgaggggcca cgcagggtca cccgcatcgc
    11641 atgtccgtcc catcgccaat cgattgagcc gccgggaagc gcgagattcg agaccgtcat
    11701 cgagtgcgag atccaggcct ggggaacgcc cgcgccgatc accactgtgg gctctgccgc
    11761 cccgtcgtcc acataggcca gcatgtcttg ctgcaacagc aagagcaacg ccgccgtctc
    11821 ataatcggga gaaacgacgg actgattgcg ccaccctctg acgtattgcc agccatccgc
    11881 cacatcggtg gtcgccggcc tcggcatatc ccacgtgtac aggccgggcg atgcctgctg
    11941 agtccaaagc cggtgcagcg cagcccagac ggcgtccggt cgcccctgtc gcaaggccag
    12001 atgagcgtcg ctcaattgcg tggcgacggt tgccgtgtcg ggagaaggcc gatagaccgt
    12061 caatgcttgc cccaattgct cgcgattcga cgttatcgaa gctgatggtc ccagactcac
    12121 cgtcgagatc ttggcatcag aggcgccatt gcggtttcgg cgttgccaat tgtcgcgtaa
    12181 cgtgtgggca tggttccgcc agcctgtcgc gtatcggtgc ttgcccagcc gttcggcaaa
    12241 ctcggccgcg gcaagcaagc cacggtagct caccgcattc acgtagagcg agggccactc
    12301 gtctccgaca cggcctacga tcagcccgtc gcgtgacggt ttggccagca cacttcgtcg
    12361 tatctgttgg ccgtgttgga ggtcgtacgg agagggcacg gcaaaggatt cttcgatggg
    12421 ggcgcgggcc gtcagcatgg cctcaatgcg ttgtgccttg cggagcacat ggggccacaa
    12481 ccactgatcg tgctcatgat cggcgatata gtctgctgac gtggtcaagg cccagatggt
    12541 caggcccggc gcatcggctt cgggaccgtt tccgccggca aaatcgtggg ttgccagata
    12601 ttgggccagc acgcggctga cgtggggatc acccgcgcgc gcgagggcgg tcgcgatata
    12661 ggccccttgc cgctgccagg cgcgatagaa aaaggtgggg tcgccgggtc tggtttcgcg
    12721 gccgatcaaa ctcatcatca ggtgagtcgt ctgtgccgcc atcgaagtct caaaccgttt
    12781 gtcgggaaag tcgagatgga tctgctccgg tgcatttcta taccaccgct gcatttgaat
    12841 gggaatccgt aagtcggaca agatgagccg gtattcgtct cccgccgctg agacctcccg
    12901 gggtagcgcg aggcgagcgt aaccccagcc ggatgcattg gcccaggacc ggttctccgt
    12961 ttcggacgtc attcctttta cggtctgctc gtctccgaga gagaccttcg caggagcagg
    13021 cgtgacgcgg acggtccaac gatgattgat gtcgagctgc tcgccgtccc agcccaattc
    13081 ggtgaccggt cccccggcgg gcccgacgct gcgaatcatg atcgcggggg tgtgcgacgt
    13141 atggttcttg aaccgcagct cccaatgggt ggcgtccagt cgtgtccagg tggcgtcata
    13201 gtagggcgtc cgggtcgcga tggcaggaac atgaggatgg gtggacggtt cgaatgtctg
    13261 ctgaatgtca ttgagcggca ccgactggct ggtgacgagc gggcgtccct cggcatcgca
    13321 gagccagagc gaaacaccga agctcggcgc ttgcggactg aagtccccac cgggctcgtg
    13381 ataggcatgt tcggcttcag gggcgccggg cacggccagc gggatatgcc cgaccgatcg
    13441 gggccagggg cggtccggat cgttctgcat caggctgttc atcgcgtcgc gttgcggtgt
    13501 gaagtgggtc gcggactccg ttgcctgccg gaattgctgc cagttgttga tgccggcgca
    13561 cagggcgagg aggccggcga ggacgagcac cgccgggcgt cccgggctga gcgtccccag
    13621 cgcggcgagg gcgatgagaa gaggggtgaa attcagactg taggtgaagg tttcttcccc
    13681 atagaccatg tgcaacgaaa gttcgaacaa caacaaggtt gccagcacga ggcggaagcg
    13741 caactggcga tccatagtca ccagccgcca gagtccgttg agaagtaagg ccgaccacag
    13801 gcagacggcg atcaacccga gcgggcccgc cgacccggga gtggaaaatt gaaacgccag
    13861 gcgttgcgcc agacgaacgg agtcgtcgcc gacctgaatg tacccatcgt catcgatgaa
    13921 ccgtatcgcc ggcgcgatga gggtgtggaa ggcaaacgac gaggcaatat tgctcgcctg
    13981 tccggactga ggatggttga tccagttcgc ttccttgcgg ctgccgatga aaaattccgc
    14041 ggacgggaag atgaactttt gcgctcccca caagagcacg accaggcaga aggcattaat
    14101 ggagagttgt accgcctgtt tgaacggcca gcgcgccagc gtcgtcagga gaccggccat
    14161 ccagttggtg acggtgatgc tgagagtgaa ggcgctggcg agcacatagg acagggcgcc
    14221 gactcgatgc tgttcggcga gcaagagcag ggcgagcgag atcatgaccg accaggagcc
    14281 ccaggtatag gagttgggca cagggagcca aaagatcgcc gaggcactgg agagcccgag
    14341 tgacgtgaag acggaggcgt cgagcgttcg gcagccaagc aggcgcagca ggagatacaa
    14401 cgtccccatc cataggcccc cgaggatgct ggtcacgtac aagaccgctt gcagcggggg
    14461 gatcgccagg gcgtgttgta ccaggtagac ggggacgaaa ctcaggattg agaacaaggg
    14521 atggacggag gttcgtgaat gatcatcggt ggtggaggtc atattcgaga cttcacgaac
    14581 cgtgtccgat tcaaaccaaa agtccatcga ttgcagcaga aaggcaggga tgctttcggc
    14641 aatgaagaag caggcgatgc tgaccgagag ggtcaggacc gcggcaacca cccagtcggt
    14701 tggttccagc cgggcggtgt gatgcggttg ttcctggacc ggggagggcg aaacctcggt
    14761 gtccttgtcc gggaactccg gctcgggata gggaagcgcg cgcgctgtat tgccgtctct
    14821 caggaaaccc atgtctattc aggtagttag aacgggggta cgatcgggcc caatcccgac
    14881 agtaagaaat cggtcgattg ggttgcggac tgaagcaggg aggagtcgcg gatcgggtac
    14941 ggaacgggga gatgccgcag caggttcggg atcgggcgcc tctctgtcct gtccgtgagt
    15001 tggagcgtca gggggtccga tgggacattg tgtatacttt tagtcggatg aacccggcta
    15061 cgccgactcc tccgggagcg acatgccggg gcctggatcg ggagaaccgg cctggttgct
    15121 gccacgcctc gggccgacaa tgtagacaaa tcctttccct gtggcgctgc gacagggcag
    15181 gtgtgatagg cggttcattc catgcatcag tatgcgatct tttatggagc ggctgctctg
    15241 gcagtggtgg tgtggtggct cacgtttcga gatggttcag cacgagcgcc ctttctcgca
    15301 atcctgggcg cagtcctggg cggtcttgtg ctttgggcct ggcaagtttc tgagccatcc
    15361 gtcctgttct cggatttcaa tgtcgcctac tacccggcgg gacgggccat tcttgaagat
    15421 atcccccatc tgttcgtgcg ttgttgggat acgccggtgt gcgggtttgt gaacattccc
    15481 attgtcgcct ttctctttac ccccttctca tttctgacgc ttcagcatgc gcagtggttg
    15541 ttgatgggcc tttcggcggt gagcctggtg atgagcctcg ctcttctctg gtcgcttacg
    15601 gatcgaaccc ggtccagagg gtgggccatg cttctcctgt tcgcgttgaa cgggccgctc
    15661 gtctacagcc tcaaagaggg caatctgact cacttcgccc tgttgctgct gattgccggg
    15721 gtcgtctgcc tggacaaaac gtgggatcga agcgcaggga gttgttttgc attggcggcg
    15781 atcatcaagc tgccgctgtt gctgtttgcg gtgtatttct tcggcaagcg gcgttgggcg
    15841 gcggtgttcg gctatggcct gaccctgctg gccgtgacgg gtctatcgat ctggtacgcc
    15901 gggtgggcca gccacgtggc ctggtatcgg gaggtgatcc tgccgctgtc cgacaaggga
    15961 attgccgcgt ttaatgtgca atcggtgcag gggctgctgc tgcgactgca ggatggtgtc
    16021 cggttgtacg attggaagcc tgtggccgtg ccctgggatg tcggggcggc agggcaggcc
    16081 tgtgcggcct tgttcgtcgg gttgtccggt tggctgtttc ttcggagtcc cggtctgcag
    16141 gttcgagaga cgacgaatct tgagttgtcg atggtgctct gtctctccct gatcatcagc
    16201 ccgatttcat ggacccacta ttacctgctg ctgttgctgc cgttgtcgtt gtatgtgggc
    16261 aaccgcctgc cggttgcaac tcatggcggc tgggcggtgg ggatgacgct gtgcctgttg
    16321 ctcatctcgc ctccggtaac gtttccaggg ccggatcaag tggccggcga ccagatcgcg
    16381 acgttgtttc tctctcatta tgtgatcggg gctatcctct tgtgggggct gtgcgggtac
    16441 gcccgttggt ctgttgcaaa tgtcagccgc ctacgactgg tggccgcagg gtcggggccg
    16501 cctgttccgg tcagggcaga tctgccgcag gagtctgacg agtcccatac gcgatctgcc
    16561 taagcaggcc cattcatgag tctcttccac gtggcccgat cctctcgtcg agcgggtcgt
    16621 ttctcagtcg acttcctcga agaacggcaa gcaggactgt ggtcaggaac atctcccagg
    16681 gcagtcagga atggccgcga tccaggaccc gttcggctcg caccacatag cgcaacgggc
    16741 cgcccgctcg gattgcatcg aaggggaaac aggtcaccag caccagatcc tctgtgtctt
    16801 gacctgtcgg aatgagctcc agccgtgaat ccatgactcg ctgttcgatg actcgatact
    16861 gcagggtggt tccgtcgctt cccgtcaggg cgatgtggtc gtccaatttc acatctttca
    16921 aaaaacgaaa atgcgtgtcc cggtgaccgg tcagcatcac cgtgccttcc tgcccgggca
    16981 gggcgctgga cgtgacgtgg ccgggaccga atgccagagt ccggccatac gcgccggcga
    17041 ggacgatcag gtctaccgac gggtgctgca tgcggagccg cgcaaccggc caggtatcgg
    17101 cccagggcca gggtttcggc atcgcctctc ccgccagggc tcggcaccag gcccgctgca
    17161 gtaggaactg cgcgagcccg gccttcgcat agatccacga tccctccccg agttgccagc
    17221 agccgatggc gagaagtccc gccatcagca ttgtcagcag gcgggagccc ggtctgagtc
    17281 gactcatgcg accgcccggc gaaattgcca cagcaagctg gccaacatga gcgcagctgc
    17341 ccccagtaga atctgcagtt gtgcgccggt cgcagtcctc gggaggcttg cgagggcggt
    17401 gaggccctgt gcacgcgcca ggttacttgt ctggtcacgc tcagttaccg gggagtccgt
    17461 cggtctggcc ggggtaacat cgacagcgac cagactggtg tatcggctga ccaggtgatg
    17521 ggccagcgcc acgtccagca cggcctttcg gatcgtttct tcagcgccgc ccttgtaggt
    17581 ctcgtccatc agcgccgcaa tctttttcct ggcccagtag accgagagcc ctccatgagt
    17641 gggggcgtcg ttgaacgaga tcgggagcga ccaggctgcg cgtccgatct gtccgcgcaa
    17701 cacgctttgg gatggcagcg agtcggcctt gagtgtgagc acgatcggtt cgccttcata
    17761 gagatccgtg atggttgccg ggaactgttc gagccccgac catccggctg catcgatggt
    17821 gatgtcgttg agcaccggat gctcaagctt tttgaacaga ccatccaact tgtccttcac
    17881 ctcgttcacg ttgccgatat aggtgaaggt gccgcggcct gtttcggcag ccttccgcat
    17941 caggtgactg ttgggcgtgg agccgattcc gatggtgaac aggcgtctgc tgcccacgcg
    18001 ttggtgcagc agttcgaaga gctcctcctc attgccgacc tgtccatcgg tgatgagaat
    18061 gatctgctgg agccgtgcgc tgtcctgcgg gctcttgagg gcctgtctca aagccggaag
    18121 tatttccgtt cccccgtcgg ccgtgagctg ttcggtgtac cggatggctt gctgcatcgt
    18181 tgtggtcgtc acgggttgcg gtatcgagaa cagcgaccgg accgtatgat tgaattgaat
    18241 gatgttgaag cgatcctgtg tggtcagccg tgcgagggcg gccgctaatg accccttggc
    18301 ctgttcgatc gacgcgccgg ccatggatcc cgatcggtcg atgatgaacg tgatgtctcg
    18361 cggaacccgc gccgctttct catggtgctg tgtcggagga acgagcatca gcagggcata
    18421 ggtcgcgccg tctttctgtt cggtaaagac ggtggccatc ggttcggtgc gcggtgccgg
    18481 atgccagatg agctggaaat cccgatcagc cggtaccgca tcttcccgga gaccgatctg
    18541 atatccgccg ccctgatcct gaatggtaat gatcgggtga aacggcgact ctaccttggc
    18601 aagaggaaaa cccgggttga gcgagagcga gaggctcagg gggttgatcg cgccgtcctc
    18661 gggtgggtgt atgggcggcg taatccgtga ggcatcgggg acacgatcag tgtccggact
    18721 ggtgccggat ccctgaagat cctgtccttc gacgaccacc gggacgccgg ggatgtaccg
    18781 cggcccgacg gccatgggga agcggagttg aaactcttca ttctcgtagc ggacggcgtc
    18841 ctgatattcg agttcgaccg tcacatgttc accgggcccg atattcgcga ctgaagtggt
    18901 aaagagattc ggacgttcct gttcgaccag gccggtccgt ttcccttccc gcttggcctg
    18961 gtcgtagagt tgtttcgcct ccgcccgttc cttgatccgg ccttcgatga tccgctcgcc
    19021 gatttttatg cggagatgat ccaccgcggc cgtctcgggc agcgggaaca cgtagacgcc
    19081 ttccagccag tcgcctttct ttttgctcgg gttggtgaac tcctgccgga cggtcgcgcg
    19141 ggcaatcgtc ccggtgatcg cgatgtgcac atcggtcttg agaacgggcg ccggtgtaaa
    19201 gcgtccggct tggtcggtcc tgaacatcaa cgtgccttcc gtcacatcat tgatgcccat
    19261 ggtggaaacg atgccggtat tgagggtttc gtccggttcg gcgcaggagg tggctggaag
    19321 gggagcgcca agtgccaatg acagaaagaa cgcacagagc atgaggcgcg ctgcagaggt
    19381 tttcgcgcgt tgtcgtagag agccgaagcg gtcagtcagc atagaatcct cgtttcgtca
    19441 tagtcgtgga tggtggagtg acggcgggtg tccccgggaa ggacgggctg tcgttcccag
    19501 ggacgtcacc ggcagccggt cagttgccgt cagtcggctc ggaagtacgt tctgccggct
    19561 cggaggtcgt gagtggaagg ccttcgctgc tcgctccgct ccccccggtt tcaattgagg
    19621 gatcgacgct gttcgagaac ggatcggtca ctgtttcagt ggcggttata ggcactatca
    19681 tgtgttcctg gccgatcctt cgcatctcca cgtgtacccg tcggttcatc cggcggcaga
    19741 cgtcgctgtt gtcgatgcag agggcgcctt cttcacccag gctcacggtg cggatcgacg
    19801 attccggcac acccagcgcg atcaattgtt gtttaacggt ctcggcgcgc ttcatgccca
    19861 ggatcttgtt gaagctcctc gaaccctgtt gatcggtata gccctggagc aggatgcccc
    19921 aatcccgctc gctcgtgagg aattccgcgt gtcgctgcaa ggcggccttg ccgtcatcgg
    19981 ttaggccctt gcggccgatt tcaaagtgaa tatcgtcgtg caggatgtcg gtacttcggg
    20041 ttgccaacgg ggttccgtcc acaagcgtcc ttggaatcga ggctgagacc ggcgcgtgct
    20101 cggtcggatc tttcagaact tgcgccacct gggcactatt gagggcttct tccgacgggt
    20161 tgctatgggc tgcctcgtcg gcctgggagt agtaccagaa tgcggacacg acgacggcga
    20221 ggaagaacac caagccgctc atgatataga catccttcat ctccttttcc ggtcccataa
    20281 cggtgggaga cactggtcct gcgttgacgc tggctaaggg ggctttctgc atgatccgtc
    20341 tgctccttgt gttgtagggc cggggtaccc cggcccgtcg gttgctgatg gatgaatcga
    20401 ctgacccttg catcgcgggg ccggcttgtt gatgctcacc tccttcctgc cgggctgccc
    20461 gccgttggcc ggtgggtaga gcaaggcgga ggccaggaga agcaacggag ctgatgattg
    20521 gaaacagtct ttaaaacaaa gagttatgaa actgttgtcg atgcgagtga acagcggttc
    20581 atgcggggat cagcggggat gcggggatag ccccgcatca ggagccgggt agatgaggcg
    20641 aataatcagc ccacctattg ggagcccgtc tcgtaacgcg ccgtcccgaa cgaactttgt
    20701 tgaaagcacg aagcggggat cgaacaggca cctctcgcga agggaaccta tacgaccatg
    20761 gcgtcgttgc tggaaatgtt ttgcgagtgt ccgctgctgc ggtaacatgg cgcaaaaagc
    20821 cgccgacacc tgttcggaac agaggcaggg catgaggagt cgtcgaatga aaaagtctct
    20881 tctactgttg ttgctgttgt gggccgcgat gttctctttg gcacaggcgg gggattacca
    20941 gttgatcatc ggcgagcagg tggacgtgtg tgaggcctgc aaacgcaatc tggaacggat
    21001 gaccgtgcat ccggcttgtg agcgggatta tagcgatcaa cttggactgg aggctcctga
    21061 gtggaagcca ttcgatgtgg gggggcatat cggtgcgatg gaacgagtgc tacggtatct
    21121 tgcaaccggt gatgagtttg ccgatgacag atataattcg gatgatgatc ggtacgcgga
    21181 tccggtacga agaatcggta ggaaggagga gccctgggcc tattgggcgt acgtcgacat
    21241 caacaacgac gggaaggcgg agccggtgct gaagctccat tcggcgctct gcaaactgag
    21301 aaatggcgga tctatcggtc aagcctattc tgctcccatt gtggtgctca atgagaatct
    21361 cagtggcatc gatcgtgcgc tgaccgatct cgtggttcag aaccctcgca aggagggaac
    21421 ggtggccggc acaaccaatt accagctcta cggggtgttc agtttcaagg gggaaatgta
    21481 cttcgaccgt tggaatgaca ctgagactgg agacgagcgc gagagcttta ccctgagtat
    21541 ttatgaagca aagggggaca ccactcgaac actgtgccaa atgaaagaag tacaggtgag
    21601 aaggtggtcg cccggtaagc cctgcgcatt ccgccactgt cccccggcga agccgtttcc
    21661 cgtgaatctc actccctgat tcaccagact gtcgccattc ttccttcacg aacctcgctc
    21721 atcgaatccc caggcgccgc agatcgatga catcagagaa ggaaaaggga gcagccagac
    21781 tctctttcct gctcgcagtg tcagtaatca tgccaaagtg acccctgtca tcatgtgaaa
    21841 tggacccctc tcccacaacc aggagaggac acccatggag ctagcggaac cagtgcgcac
    21901 cccactacct tcttctcggg agacggctcg aacggaggag g
//
LOCUS       DAORRQ010000067        20913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_150_length_20913_cov_1211.530252, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000067 DAORRQ010000000
VERSION     DAORRQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20913
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150_length_20913_cov_1211.530252"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PK224_16740"
     CDS             <1..336
                     /locus_tag="PK224_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17445.1"
                     /translation="GVVERQALEALVQHYVRPLATRLHNEMTRLWKEGAPGIDHLLYC
                     GGGSALLASYLGHFRDDYTILPESQFTNAAGYLEYIRLTSTDQTRAQDQGAEDSPQGD
                     ASAQKASHA"
     gene            complement(765..5750)
                     /locus_tag="PK224_16745"
     CDS             complement(765..5750)
                     /locus_tag="PK224_16745"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRB17446.1"
                     /translation="MPSTVQTIHSILGQFREEARSNRDLGDRFERLICRYLELDPIYA
                     ERFSNVWMWNEWPRKGKVGDVGIDVVAEERATGEFCAIQCKFYLPEHTIAKEDIDSFF
                     TASGKQLFTSCMIVSTTDKWGKNAEDALINQSKPVTRLTIHDLDASPIDWSKFSLRHP
                     QALSLRAHKKTREHQTAAIRDVVKGFSQGDRGQLIMACGTGKTFTALRVAEKMAPTGN
                     VLFLVPSLSLMSQSLREWTAESVRPIHSLAVCSDTQIGKSTTKAKDDKGDITTYDLAF
                     PATTSARQIIRQHRAIHSMAERKKTPTQMTVVFSTYQSIDAVSQAQKAGLPGFDLIIC
                     DEAHRTTGVTLSGEDESHFVKVHDADFIKGKRRLYMTATPRVYGDEAKVKAGQAEAEL
                     CSMDNIEQFGPEFHRLGFGEAVSKALLSDYKVLVLAVDEKYVSKTFQRQIADTNNELN
                     LDDAVRITGCWNGLAKKFDQASATMATTELQGDTIPMRRAVAFSRSIKDSKAFVEKFA
                     QIVTAYRETHPEADDLLQCELDHVDGTFDALKRNALLDWLKADSPGNTCRILSNARCL
                     SEGVDVPALDAVLFLNPRKSVIDVVQSVGRVMRRAEGKKYGYIILPIGIPADVPAEEA
                     LKDNEKYKIVWQVLQALRAHDDRFNATINQIELNKQRPDAIQVIGIGESDPASRDDSK
                     SAGDGQMKAQQVQGRFAFPELEEWKDAIYARIVLKCGDRRYWESWAKDVAGIAARHTD
                     RIRALLESGDKRHRRAFADFLAGLRDNINPSINENEAIEMLSQHLITRPVFDALFENY
                     AFTQHNPVSIAMQQMLEILHDQALEKETATLDKFYASVRQRVSGIDNAKGRQDVVIEL
                     YDKFFRTAFPKMTERLGIVYTPVEVVDFILRSVEDVLRDEFNSSLSVENVHIIDPFTG
                     TGTFIVRLLQSALIQPADLLRKYQKELHANEIVLLAYYIAAINIEEAFHRVQRAEGSS
                     SSSGFSVPSALEYQPFEGIVLTDTFQLSESKGGLDEKMFPENNRRVKRQKLSPITVVV
                     SNPPYSVGQGDANANNQNPKYPTLDDRIRSTYAANSSATNKNSLYDSYIRALRWASDR
                     ISEKGVVGFVSNNSFIDGNALEGLRASLFREFTTIYVFNLRGNQRTSGETSRMEGGKI
                     FGSGSRAGIAISLFVRNPAKTGECELYYHDIGDYRSREEKLRIIEKFQSLNGLHSKRS
                     WRHIHPNASYDWINQRDPAFERFVSIGDKKDDSVKTVFETYSRGLETARDAWCYNFSR
                     FALTSNMERMIRFYNEQVDGFAEYSKGKPRGEFPELAKKFIDTDAKKISWSSSLILDL
                     QRLRKTRFHGTAVTQAVYRPYCKVNAYFDQDMIHRVGQFPQIFPRASSANLVICMTGV
                     GNRIDFSVLITDSVPDLHMADSNGASQCFPLYLYEEEKLQEGLLIRHEPKGELVDGYR
                     RSSAIADAILIDFRAAYEARITKEDIFYYVYGVLHSPEYRTRFASDLKKMLARLPFTC
                     ETANFWRFSDAGRDLARWHLKYETVEPWPVNESTGELFLEPEKDFLVQQMTFGRATAE
                     QKARGEKVDKTTIHYNSRIMLTNIPLEAYDYVVNGKSAIEWIMERYQVTVDKDSGIRN
                     DPNDWAREHKQPRYILDLLKRVIAVSMETMKIVQSLPAVNERIDDPAMPVV"
     gene            6045..6407
                     /locus_tag="PK224_16750"
     CDS             6045..6407
                     /locus_tag="PK224_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB17447.1"
                     /translation="MAWDVEFYADFALEFDELDEEVQDELLAHLHLLAEFGPVLGRPW
                     ADTLKGSRHANMKELRFNAADGVWRVAFAFDRRRRAVLLVAGDKSGGSEKQFYRELIR
                     KADNRFDRHLGATRKRKD"
     gene            6436..6753
                     /locus_tag="PK224_16755"
     CDS             6436..6753
                     /locus_tag="PK224_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB17448.1"
                     /translation="MKKLPAARRKMVEARAAELIAQEMTLRELRQAHRRTQERIAETL
                     GIGQEGVSRLEKRSDLLISTLRSYIEAMGGSLSIIAKFPDHPPVEVAGLTAIDAEREP
                     VRS"
     gene            6766..7563
                     /locus_tag="PK224_16760"
     CDS             6766..7563
                     /locus_tag="PK224_16760"
                     /inference="COORDINATES: protein motif:HMM:NF025713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HRB17449.1"
                     /translation="MTIIERGYLEKLFHMDSGYVLTFSDTTFGHFMADAVGIDIHNHK
                     YQSQGSSKAKKLRAFWTLEPDHLAGKAVTALIEYIEAHPLSDEISSEQNKLIETCKSI
                     GHRLLAGKVNFDPLKQTAAAFEARHLAEQIRRMEQCVQSDPALAIGTAKELIETCCKT
                     ILAQRGKSLPGKPDIPELTKATLKELKLVPDAVEDSARGSDIIKRLLQNLGTIGNNLA
                     ELRGLYGTGHGKLAHSETLTPRHARLAVGAASTLTTFLFDTHRETAS"
     gene            complement(7653..8393)
                     /locus_tag="PK224_16765"
     CDS             complement(7653..8393)
                     /locus_tag="PK224_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17450.1"
                     /translation="MEQSASRRGPIFAEGASGRSRPRSLIAAISEIQELAVVEKLGTP
                     LPEHLILLDRSFAYTEVGVHSVVVDGLINMLGVPMGLGVMGSLMPIFGDPDPSLFDQV
                     FAQLIGITYGVGYAVMIGHNLGSCYIGKVCKRAIQDIYQSMIVANIIKVILLSVFFNF
                     MYQLLTPEHVQEGFKVLWPLLRGVMSDQMAVGVQDWVISMREVMIPSATVVILVTIVT
                     LCVPYVYFVIGGREAAEEKRRNRLYDVC"
     gene            complement(8396..8614)
                     /locus_tag="PK224_16770"
     CDS             complement(8396..8614)
                     /locus_tag="PK224_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17451.1"
                     /translation="MDPETFIAVSSWVNTTLLAGVPLRILASATLAATFWFGAYKQRI
                     ALGFLFFMLTILLSYFRPLINLIGSGSM"
     gene            complement(8625..9599)
                     /locus_tag="PK224_16775"
     CDS             complement(8625..9599)
                     /locus_tag="PK224_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRB17452.1"
                     /translation="MPYQPVIESQRALEPLLTVVKILEEYGDCPSIVTAGIERDEECT
                     DLYSIRDTLAKITLRDHTYRVTELALALLKQTYRDGDLLIPKVLVATLGHDLGKIPRF
                     RATAAHAMGDHPVVSAIKLQECFAGTSIPWFSEVLDAIKGHHRIGKDRLGVILRQADG
                     QARVKEMILSTQEMQEKPLDSWCAGPEVLAIVAPRINRPLKGSKWAAFSLKGVVYVTP
                     DAILEAAKELARQKKVVEMGLIRSTDREDTLRRLVKILGAADLLAMEIGEHFYGRPFD
                     IFTKKAGIKQRGYFVPVKLEAFQIAESELESRKVAFMQLVTEFQLGRG"
     gene            complement(10006..10458)
                     /locus_tag="PK224_16780"
     CDS             complement(10006..10458)
                     /locus_tag="PK224_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17453.1"
                     /translation="MSDRQTEACSTENALHARWAFDVTPGRERHVALQLQELGCTDVE
                     RPTKGYVIAHCDPTAVPVIKRLSGVVRALPLLDSDLFEDLSNVEPLMAKVADVVQVSS
                     GPYAQMTGIVRALDGEDVLVDVSLMGRVVRVRAKATHVSVMPLPEPWR"
     gene            complement(10500..11006)
                     /locus_tag="PK224_16785"
     CDS             complement(10500..11006)
                     /locus_tag="PK224_16785"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRB17454.1"
                     /translation="MPDSVAAEMPPDRFWYVNHSCVVAAANRYAVTVQILEAIILVES
                     EGDPHAVNVNRDGKGDRRGPLSFKQATDLVAELWKAGANFDVGIAQINSVHMRQYKID
                     PVHFLDPCINIQWAAFVLRQKINEYQETWTAVGRYNGSRNIAGYSWKVYRALERLAGI
                     RRARGLLR"
     gene            complement(11099..12685)
                     /gene="traN"
                     /locus_tag="PK224_16790"
     CDS             complement(11099..12685)
                     /gene="traN"
                     /locus_tag="PK224_16790"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HRB17455.1"
                     /translation="MTTAQSTAATLPTDSIMTESCSVVNMCTSYTEGAGGTGTCQVPG
                     GSRQTCLKIRNPTLLPNACTVPGIPSPPYTSETEVNGCKDLEAAGASGLAALVTTTCL
                     DGAARDLKCAQPYATVLTGGTSGFNAHDHAPGMAMGNFTIESAFPIWDGTRWVVGLNV
                     TGNSAGEDKYGCTNVGSVKTGPYVLTPANPTASGNGSPAYCNSKCSCFGYPMTFTMGA
                     FSPTGPLSWQVPFTFTYESHSIAVMVTGTFLDTYSVAPLTGCFQEEQTWDVTSTAGDT
                     CGEYRDKGCNQVSSRCASVQPGTGYCLMYENTYKCPGPSECSTTQPITQCTKCGKPDS
                     PVPFCVDTSTPPNENLALAATWLAMTKNVEEDWDPDRLRIFTGKRSTCDFSTIGRSLI
                     NCCDSDPDKLIGKCSEEEIALARDKHDRKAHLIGTHCVDKGSFLVGSVCLRKEEVYCT
                     MKSELGRMVQEQGRVQLGMTWGSADAPQCDGFTVDQFSALNFQAMDFTEWYKNVSANI
                     DPAVITKEMATKICTYTGTC"
     gene            complement(13071..13562)
                     /locus_tag="PK224_16795"
     CDS             complement(13071..13562)
                     /locus_tag="PK224_16795"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRB17456.1"
                     /translation="MKILTVDVIPGHTYRYFQALIQIETVGIASWRDAVVRFTDFWGG
                     RAKTYDSPIVHATQSMVNRLAQSAAALGADLIIGFDIQIHQVTARYWGWGMAQIIMRG
                     TPIQFMDRDALGTILAGELSPLVARDQSARHPPDGNSLGEAVGMDEMTVPSLDTARLG
                     TRR"
     gene            complement(13569..14540)
                     /locus_tag="PK224_16800"
     CDS             complement(13569..14540)
                     /locus_tag="PK224_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17457.1"
                     /translation="MWQRMAALSLALLSGCVSTGEYQDLETKYQHQLAVKEVMETEHD
                     ELSQRVDNVSQVYRHVAQEQMGLKAQAEKLQGDILTVKGVVKDTQNQYESQRAQFAQQ
                     AQQLSGVDARLQNIAGKIETLTETTVALANRFERVELSIGKLGKSLQARGGAPDVQKT
                     RVQLGEGKSGSAPKTEPVAKAGSPNMEGSVGAGGSGVEPSGTSLAVAPSPVATTTVPA
                     KPDVPMPVPQRVIEAKVESPRTKPDADKGWLRRFLDGFRGGNGQNAELVPPPLASGMS
                     GKASSGPPLLPAVDGEVMAAPMNTLTISIDADAPKKAEVSPSDTEKR"
     gene            complement(14585..15229)
                     /locus_tag="PK224_16805"
     CDS             complement(14585..15229)
                     /locus_tag="PK224_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17458.1"
                     /translation="MAHGVDAERQLGMVRREGQSLRSTSPTNGRRPDRAEENKRRLED
                     PNSVLLLPQTKAGMACVSMLYMFDKVIHRLRRAAGSQLSIEQVRNELARVEQWVSRAK
                     VILRELGVGEFELLPQDPYPDLKERSVKAAWRNARVITPRVGDTVRLVPVIRALDNAL
                     NSIRLRAEDLTPYVKHFQAVDELVVALHDVIGHVARVTVTEYEPRGAVKAFLNQ"
     gene            complement(15245..15874)
                     /locus_tag="PK224_16810"
     CDS             complement(15245..15874)
                     /locus_tag="PK224_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17459.1"
                     /translation="MARVIRMDPGHSAYLLWADSIGRYAILPHQYADRQYKVRDSLMG
                     AVYKVGDEFPMLSQRSMHYFRRIAELVLAPVLADDHVHIKRVATAQAASFVKIAVETY
                     RTGEDVIATCKPLLYEFRTYTSRTIALVKYHTEVEAYIREALAPAPSRSVRRVDVYRE
                     EHQARVIVERGTIAQFLGRKGMNAAVASKLTGYGLVLVDEADLGKTARA"
     gene            16251..17279
                     /locus_tag="PK224_16815"
     CDS             16251..17279
                     /locus_tag="PK224_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17460.1"
                     /translation="MRVTQTVEEKNRIKREEVWGGYTYERRLNLVTMVGGGLLCAPNL
                     LLNTLFSMGTPNQPGWSSPWQYCLAAAGYDVPGRADTRTRIADDLDPQVQTVTRIIVD
                     GDLRLTFAADGRDRIGIAVPVSKDSQGTHIRLRWLAMALREQGVNPADVPDGTVEISY
                     SPTVGNPGPTTQLPIDGHVLTAMLQHDLAVLPQDRWPKQPRLRLAVNNDWGHEQSQLI
                     VDRLIGRMKERGLPLVIRNERYRKLVPVQVRQLAPFFNDERQPSAGMGTGANLLVSID
                     IASASDQGLLLTTTLVSVETAQVLAQFVLECPINAVAPTAETLAAVVTVLVLPHGPTL
                     TRGWFVTP"
     gene            17276..18400
                     /locus_tag="PK224_16820"
     CDS             17276..18400
                     /locus_tag="PK224_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17461.1"
                     /translation="MTRYGTFLICFLATSAVGCSSLILSNTPVQGENTSTREIARTPI
                     DVPEVTLTPHHDQQRLTIRVTQRSQITLETDTPIKVEARRYVFSPTAPLAGAVHCPVL
                     TVAYVISAGHLGSQSLAPACRRLLMQEPLAGSPRVATEIEHRYETHTTTEPVRYASLR
                     VAWDDAIHPPLVYDVNPDGEVSIPQHALRSGTERDPSTLPTLSILVDNRVLWTHRLTP
                     SEMTPSGPLPDEPHTAGTSWPPIVALVLEPLDGAAPPTLRAWGRLVTQALLERHICVV
                     APITFAKDLQDELLLHQSGRISDQTAVHFGKWIPATVALTIRKGAANQGIAILEFVDI
                     QRAERIGYDVLRYSSDWKEDAQMVKAYMQTIFPKRYQSCS"
     gene            complement(18406..18801)
                     /locus_tag="PK224_16825"
     CDS             complement(18406..18801)
                     /locus_tag="PK224_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17462.1"
                     /translation="MRLLRARIFLSLSLLFLCSAHHSLAETRTDSWLGAFVGSAIPGK
                     VPGHSTIAVLSKRFRPSFEGNALAVLDELVSYCRDMTKGRALLNTRIETSVGDMSKSG
                     SDKQAITMYPGSLLITVYGDCVLEVQDAK"
     gene            complement(18839..>20913)
                     /locus_tag="PK224_16830"
     CDS             complement(18839..>20913)
                     /locus_tag="PK224_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17463.1"
                     /translation="GTDASGRQFLVEATEHFESGTSGVAHDTHTVGSYTLSGTFTALS
                     HGEANQPGVPTAFFGSAIDNSKPGSPAFQANALLYDGQVSFFDGREGGTKSISGERNL
                     VDGTHLSGQFSQLNVMGQDGHQVEMFHGYAQQPGAPARDVTGLIDNGSLVSFKGASGN
                     EFRDVIERTDSGDLQSIGAQETYSAPGGTHSLSNFQGVTVNPQGDAFKRSSTNLDGVS
                     VYSDQMRGSRQMTVEFEQSLRGYNNEVQSIDKESGWRRINDGHRDMDVYANLYYGPAS
                     SETGERKLLGGTITNVTDNTYSTFMKDGEGNEVWGIARSLEDPNSGAMSGSIDQTVKV
                     VAAKHGAIDTQVLGPMKDDGGQREVLYHSVLGGLDSQYYNNERKSGIFTVPDAMHPGQ
                     TKELQGTFYYSPQSGQLIASEVTDLQTGKIGQYRTDAQGQEHFQVVEYSQSPDGASVI
                     ANTKELSKNEYAAHGFAVNDVFDGSGNVLSSSGHKGSDFHDFNRVQFHHDSGANLTIT
                     QMAFAAKGTDITNPTQTDEAIAYGLEFGRIGMDGASRYLILKGRMGKFGGGGVGGTPV
                     QPGSGPVPNGPGGGGGPSGLLDSHQNMGAWWKEYSGVMDNAISFARSSVEHLEKTGRA
                     QSMSPEELQNFAANAAYNAVRTSALGMMGEEASSAFGTMPRWQEGQESLLKISKPSSQ
                     GVNTGSNK"
BASE COUNT         4674 a         6267 c         5223 g         4749 t
ORIGIN      
        1 ggtgtggtcg agcggcaagc cttagaggcg ctggttcagc attatgtgcg accgttggct
       61 acgaggctcc acaacgagat gacacgactg tggaaggaag gtgcaccagg catcgatcac
      121 ttgctttact gtggcggcgg atcagctctg ctcgcttcct atctcggcca cttcagagac
      181 gactatacga ttctgccgga gagtcagttc accaatgcgg ccggctatct ggaatacatc
      241 cgcctcacca gtacagatca gacacgcgcg caggatcagg gggctgagga ttccccacaa
      301 ggtgacgcgt ctgcacagaa ggcctcgcat gcctagagtc acagtttatt tctcggagaa
      361 gtcagcggac gatatgcgac tgtttcggtg gctggttcgg cttcccccgt atcaacgcag
      421 ccgccacttc aagcggttgg cgttggctcg cctcactaat ggggcgggcc gtccaggggc
      481 gaaacggaca gggctgctag ctcacgcagc accgaaacct aggccccgtg aggataccat
      541 cccttcaacc tcgacattcg taccgatgag tcttgtggag actcaggccg cgttctttcg
      601 ggcgatcaag aaaccctaac aggattcatg agcgtaggct gaagtatggt actccaatga
      661 cgaccatttg aacgagggta gatctcgcaa aatcggacgc cgacactccg atatagtgga
      721 ggctattgta gcttcgtatc cgcaggttgc catacttgac ctcttcaaac caccggcata
      781 gctggatcat ctatgcgttc gttgactgcg ggcagtgact ggacgatttt catcgtttcc
      841 atgctcacgg caattacgcg cttcagtaga tccaggatat agcggggctg cttgtgttcg
      901 cgtgcccaat cattcgggtc attccgaatg ccactatcct tgtccaccgt cacctggtag
      961 cgctccatga tccattcgat ggcggacttg ccgttcacaa cataatcgta agcttcgagt
     1021 ggaatgttcg tgagcataat ccgtgagttg taatgtatcg ttgtcttatc caccttttcg
     1081 ccccgcgcct tttgctcggc agtggcgcgg ccaaacgtca tctgctgcac gaggaaatcc
     1141 ttctcaggct cgagaaaaag ttcgccggtt gattcgttta caggccatgg ctcgacagtt
     1201 tcatacttca gatgccagcg cgcgaggtcg cgacccgcat cgctgaagcg ccagaagttc
     1261 gccgtctcac aagtgaatgg caatctcgcc agcatcttct tcaagtcgct agcgaagcgt
     1321 gtgcgatact ctggcgaatg aagtacacca tagacatagt aaaagatgtc ctccttggtg
     1381 atccgcgctt cgtaggcagc tcgaaaatcg atgaggattg catccgcaat ggcactactc
     1441 cgtcggtagc cgtccaccaa ttcgcctttt ggctcatggc gaatgagtag gccctcctga
     1501 agcttttcct cctcgtaaag gtagagtgga aagcactggc ttgccccgtt cgaatcggcc
     1561 atatgcagat ccggaaccga gtctgtaatt agcaccgaga aatcaatccg gtttcccaca
     1621 ccggtcatgc agattaccaa gttggcagag ctggccctag gaaaaatctg cgggaactgg
     1681 cctacacggt gaatcatatc ctgatcaaag taggcgttga ctttgcagta tggccgatat
     1741 acggcctgcg tgacggcggt accgtgaaat ctcgttttgc gtaaccgctg caaatccagg
     1801 atcaggctgc ttgaccacga gattttcttt gcatcagtgt caataaattt cttggcgagt
     1861 tctggaaact cgcccctagg tttcccctta ctgtattctg caaaaccgtc cacctgctcg
     1921 ttgtagaagc gaatcattcg ctccatattc gaggtcaaag cgaatctcga aaagttatag
     1981 caccaagcat cgcgtgcggt ttccaaccct ctcgagtagg tctcaaaaac ggttttgacc
     2041 gaatcgtcct ttttatcgcc gattgagaca aatctctcaa aagccggatc acgttggtta
     2101 atccaatcgt aactagcgtt gggatggatg tgcctccagc tcctttttga atgaagaccg
     2161 ttgaggctct ggaacttttc gataattctc agtttttcct cgcgagagcg atagtcaccg
     2221 atgtcatggt aataaagttc gcactcgcca gttttggcgg gattccgaac aaataatgaa
     2281 attgcgatgc cagcgcggct gcctgaacca aatatttttc cgccctccat tcgggacgtt
     2341 tcgccagagg tgcgctggtt ccctcgaagg ttaaaaacat agatggtggt aaattcgcgg
     2401 aacaagcttg cccgtaaccc ttctagcgcg ttgccatcaa tgaacgaatt gttcgagacg
     2461 aaaccgacga cgcctttttc gctgatgcga tcactagccc atcgcagcgc acgaatgtaa
     2521 ctgtcgtaaa gactgttctt gttcgttgca ctggaattag ccgcataggt gctacgtata
     2581 cggtcatcaa gcgtgggata tttaggattc tgattgttgg cgtttgcatc gccctgtcct
     2641 acagaataag gcggattact caccactact gtaatcggac ttagtttttg acgttttact
     2701 cggcggttgt tttctggaaa catcttttca tccaacccac ccttgctctc agagagttgg
     2761 aacgtgtcgg ttaaaacaat accctcaaat ggttgatatt cgagagccga cgggacagag
     2821 aagccactgc tagagcttga gccttctgcg cgttgcacac gatgaaacgc ttcttcaatg
     2881 ttgattgcgg cgatgtagta agcgagcaag acgatttcgt tggcatgcag ctctttttgg
     2941 tattttcgca acaggtcggc aggctgtatg agcgcgctct gaaggaggcg cacgataaac
     3001 gtacctgtgc cggtaaacgg gtcgatgatg tggacatttt ccacgctaag cgacgagtta
     3061 aactcatctc ttaagacatc ttctacgctc cgaagaatga agtccacaac ttcaaccggc
     3121 gtatagacga tgccgaggcg ctccgtcatt ttcggaaagg cagtgcggaa aaatttgtca
     3181 tagagctcga taacaacgtc ttgacggccc ttggcattgt cgatgccgga aacacgctgg
     3241 cggacgctag cgtagaactt gtcaagcgta gcggtttcct tttccaatgc ctgatcgtga
     3301 agtatctcca gcatttgctg catagcaata gacacaggat tgtgctgggt gaaggcgtag
     3361 ttctcgaaca gggcatcgaa taccgggcgg gtgatgaggt gctgagacag catctcgatg
     3421 gcctcgttct cattgatgct gggattgatg ttgtcacgca acccggcgag gaaatcagcg
     3481 aaagctcgtc ggtgcctctt gtccccgctt tctagtaggg cccggatgcg gtcagtgtga
     3541 cgcgcggcta ttccggccac atctttggcc caactttccc agtaacgacg gtcgccgcat
     3601 ttcagaacaa tccgagcgta gatggcgtct ttccattctt ccaactccgg gaaggcaaat
     3661 ctgccttgca cctgttgagc tttcatctga ccgtcgccag cgctcttact gtcgtcccta
     3721 gaggccgggt cactttcacc aataccaata acttgaatgg catcaggacg ctgtttgttc
     3781 agttcgatct ggttgatggt ggcgttaaat cggtcatcgt gggcgcgaag cgcttgcaaa
     3841 acctgccaga cgattttgta tttttcattg tcctttaggg cctcctcggc gggcacatcc
     3901 gcaggtatac cgatgggcag aatgatgtag ccatatttct tgccttctgc gcggcgcatc
     3961 acacgaccta cggattggac aacatcgatc acggatttgc ggggattgag aaacagcacg
     4021 gcatcgagtg cggggacatc cacgccttca gacagacagc gggcattgga gagaatccgg
     4081 cacgtgtttc ccggagagtc ggccttcagc cagtcgagca aggcgttgcg cttgagagcg
     4141 tcaaacgtgc cgtccacgtg gtccagttca cactggagga ggtcatccgc ttcgggatgc
     4201 gtctcacgat aggcggtaac tatttgggca aacttttcga caaaggcttt cgaatctttg
     4261 atggatcgag agaaggccac ggcgcgacgc atcgggatgg tatcgccctg cagttcggtg
     4321 gttgccatgg tggcgctggc ttggtcgaat ttcttggcga gaccgttcca acagccagtg
     4381 attcgtacgg catcgtcgag gttaagttca ttgttggtat cggcgatctg gcgctggaac
     4441 gttttggaaa cgtatttctc gtccacggcg aggaccagta ctttatagtc ggaaaggagc
     4501 gccttgctta cagcctcacc gaagccaagc cggtgaaatt cagggccgaa ctgttcgata
     4561 ttatccatcg aacagagttc agcttctgcc tgacctgctt tcactttggc ctcatcgcca
     4621 tacacgcgcg gcgtggcggt catgtacagg cgcctctttc ccttgatgaa atcagcgtca
     4681 tggaccttca cgaagtggct ttcgtcctcc ccgctcaagg tgacgccggt ggtacgatgc
     4741 gcctcgtcgc agataatgag atcgaatcct ggaagacctg ccttctgtgc ctgcgagacg
     4801 gcgtcgatgg attggtaggt cgagaagacc actgtcattt gtgtgggcgt cttcttgcgt
     4861 tccgccatgc tgtgaatggc gcgatgctga cggatgatct gccgtgctga agttgttgcg
     4921 gggaaggcta gatcgtaggt agtgatgtcg cccttatcat ccttcgcctt ggtcgtactt
     4981 ttgccgatct gcgtatcgga acacacggca aggctatgga tagggcggac agattcagcc
     5041 gtccattcgc gcaggctctg ggacatcagc gatagggatg gcacaaggaa aaggacgttg
     5101 cccgtaggtg ccattttctc ggcgacacgg agcgcggtga acgtcttacc cgttccacag
     5161 gccatgataa gttgccccct gtcgccttgt gagaatccct taactacatc cctaatggct
     5221 gcagtctgat gctctcgcgt cttcttgtgc gcgcgtagtg atagagcctg gggatggcga
     5281 agactgaatt tcgaccaatc gatcgggctc gcatcaaggt catggatggt cagtcgtgta
     5341 acgggctttg attgattgat cagcgcatct tcggcattct tcccccactt gtctgtagtg
     5401 gaaacaatca tgcacgacgt gaatagttgc ttcccggacg cggtgaagaa ggaatcaatg
     5461 tcctctttgg caatcgtgtg ttccgggagg tagaacttgc actgaatggc gcagaattcg
     5521 cccgtcgcgc gttcctcggc gactacatcg atgcctacat ctccgacttt ccccttgcgc
     5581 ggccattcat tccacatcca aacattggaa aacctttcgg catagatggg atcaagttcg
     5641 agataacggc agataagccg ctcgaaacga tccccgagat cccggtttga tctagcttct
     5701 tcccgaaact gaccgaggat ggaatgaatc gtctgaactg tggagggcat tagatcagct
     5761 cattggccaa agatagcgtc tatcggggcc atcattctca gcgcccgacc accccggcta
     5821 tataccacct cttcttagag tccgcaaact cccttggccc aacgagatcc aacgggttat
     5881 tcaaataaaa agacccatag cacgcccgca cgctgcccct ctgtgatcat tgccaccaca
     5941 ttcggtaaca ccgcgggatg catgtttaag tcttcatgca cgggaatctc ttaacggcat
     6001 atgatgtcat tgacaccata tgcatttcac ggcatacttg ttcagtggca tgggatgttg
     6061 agttttatgc agacttcgcc ctggagttcg acgagctgga tgaggaagtc caggacgagc
     6121 tgctggctca tcttcacctt ctagcggaat tcggccctgt actcggacgc ccatgggccg
     6181 acaccctaaa aggctccagg catgcgaata tgaaggagct gaggttcaat gccgcggatg
     6241 gggtctggcg ggtggctttc gcctttgatc gccgccgccg tgccgtcctg ctcgttgccg
     6301 gagacaagtc aggcggaagt gaaaagcagt tttacagaga attgatccgc aaggctgaca
     6361 acaggtttga caggcatctc ggcgcgacga gaaaacgaaa ggattgacat catgactacc
     6421 acactgaagg ataaaatgaa gaagctgccg gcggcgcgca ggaaaatggt tgaagcgcgc
     6481 gcggctgagc taatcgcaca ggaaatgacc ctgcgcgaat tgcgccaagc ccacaggcga
     6541 acccaggagc gcattgccga aaccctcggc atcggacagg aaggagtgtc ccgcctcgaa
     6601 aagcgcagcg acctcttgat ttcaacactg cgcagctaca tcgaagccat gggcggcagc
     6661 ctgtcgatca tcgcaaaatt cccagatcat ccgcctgttg aagtcgcagg gctgacggcc
     6721 atcgatgccg agagggaacc agttcgctcc tgaaatatga gttccttgac aatcatcgaa
     6781 agaggctacc ttgaaaagct gttccacatg gacagcggct atgtactgac attctccgat
     6841 acaacatttg gtcactttat ggccgatgct gtaggcatcg acatccacaa ccacaaatat
     6901 caatcccagg gatcatcaaa ggccaaaaag ctccgcgctt tctggacatt ggagccagat
     6961 catctcgctg gcaaggcggt taccgctctt atcgaatata tagaagctca tccgctctcc
     7021 gacgaaataa gctcggaaca gaataaactg attgaaacct gcaagagcat tggccatcgc
     7081 ctccttgcgg gaaaggtcaa cttcgatcct ctaaagcaga cagccgccgc cttcgaagcc
     7141 cgccacctcg cggaacaaat caggcgcatg gaacaatgcg ttcaatccga tccggctctc
     7201 gccatcggaa cggccaaaga gctgatcgaa acttgttgca aaaccatcct tgcacaacgc
     7261 ggcaagtcac ttcctggaaa accggatatc cccgaactta ccaaagccac cctcaaggaa
     7321 ctcaaactcg tccctgacgc tgtagaagat tcggcgcgcg gcagcgacat tatcaaacgt
     7381 ctccttcaaa atcttggcac cattggaaac aacctcgctg agctgcgcgg cctttacggc
     7441 accgggcatg gcaagcttgc ccattcagaa acattgactc cccgccacgc caggctcgcc
     7501 gtcggcgcag catccactct caccacgttc ctattcgaca ctcacagaga gacagcaagt
     7561 tgaccatcgc tgagaacatg cagcggatgg atcacaacca cgatgctcgc ttgacgtgac
     7621 ggcctcttcc gtcttcgtag acatgagcgt actcagcaga cgtcatacag gcgattccgg
     7681 cgcttttctt ctgccgcctc ccgtccacca atcacaaaat agacataggg aacacagagc
     7741 gtgacgatag tcaccagaat gactaccgtg gccgatggaa tcatgacctc acgcatgcta
     7801 atcacccagt cttgcacccc gaccgccatc tgatcggaca tcacccctcg tagtaacggc
     7861 cagagaacct taaagccttc ctgtacatgt tctggggtca agagctgata cataaaatta
     7921 aagaagacgg acaggaggat caccttaatg atattggcca cgatcatcga ctgatagatg
     7981 tcctgtatcg cccgtttaca aactttcccg atgtaacatg acccgagatt atgcccaatc
     8041 atcaccgcat accccacgcc gtaggtaatc ccgatcaact gggcgaacac ctgatcgaag
     8101 aggctcggat ctggatcccc gaagatcggc atcaagctcc ccatgacacc gagtcccatc
     8161 ggcaccccta gcatattgat cagcccatca accacgacgc tatgcacccc cacctcagta
     8221 taggcaaagc tgcgatcgag cagaatgaga tgttctggga gcggcgtacc aagcttctcc
     8281 accaccgcca actcctgaat ctcgctgatc gctgcaatca gacttcgggg acggcttcgg
     8341 cctgaagctc cctccgcaaa gatcggtcct cgccgcgaag ctgattgttc cattgttaca
     8401 tgctccccga accgatcagg ttgatcagcg gccgaaaata cgacagcaga atcgtcagca
     8461 tgaaaaacaa gaatcccagt gcaatgcgct gtttgtaggc cccgaaccag aacgtcgccg
     8521 ccagagtggc agaggccaag atgcggagcg gaacaccggc cagcaacgtc gtattgaccc
     8581 acgatgacac ggcaataaac gtttccggat ccatacctgt actcctatcc tcgaccgagc
     8641 tggaattcgg tcaccaactg cataaacgca accttgcgag actcgagttc cgattcggcg
     8701 atctgaaacg cttcaagttt gaccggcacg aaatagccac gctgtttgat gcccgctttc
     8761 tttgtgaaga tgtcgaacgg ccggccgtaa aaatgctcgc caatctccat agccaagaga
     8821 tccgccgcac ctagaatttt caccagtcgc cgcagcgtat cctcgcgatc ggtcgatcga
     8881 atgagtccca tttcgacgac cttcttctgc cgtgccaact ctttggcggc ctccagaatg
     8941 gcatccggtg tcacatacac cacccctttc agcgaaaacg ccgcccactt gcttcctttg
     9001 agtggacgat tgatccgagg cgccacgatc gccaagactt ctggtcccgc acaccaactg
     9061 tctaacggct tctcctgcat ctcctgcgtg ctgaggatca tttccttcac gcgtgcctgc
     9121 ccatcggctt gccgaagaat cacgcccagc cgatccttcc cgatccgatg atggcccttg
     9181 atggcatcca acacctcact gaaccatggg atggacgtgc cggcaaagca ctcttggagc
     9241 ttaatcgcgc tgacaaccgg atgatccccc atcgcatggg cggccgtcgc acgaaagcgc
     9301 ggaatcttac ccagatcatg tcctagtgtg gccaccagta ccttcgggat cagaagatct
     9361 ccatcccggt aggtctgctt gagcaacgcc aacgctaact ccgtgacccg atacgtatgg
     9421 tctcgcagcg tgattttcgc gagcgtatcg cgaatggaat agaggtccgt acattcttcg
     9481 tcgcgttcga taccggccgt cacgatcgac ggacagtccc catattcctc gagaattttc
     9541 accaccgtga gcagcggctc cagtgcgcgc tggctctcaa ttactggttg ataaggcatg
     9601 atgaaatccc gaataagtgc ggacgaccgg ccagacgaca ccgggcgtgt cgaggtggcg
     9661 ggatcgagtg tggcggcctg tgcgcctccc tgaggaggcg gccccggctg ggcagaggac
     9721 gggccctcca cagaagttga tgcactgggg tatgtcaggg ccgcaggagc gacggccacc
     9781 tcaggatcag gcggtgcata ctctgcttga ctaccctcgg gctcagcttc ctcgaccagc
     9841 tccccagttt gttgaatgaa atccaaattg agcggaagcc acacgtcttt agccagattc
     9901 tctagggtga tttcgatcag cctcggcacc acaagcggag ttgctactcc ggtatgaact
     9961 cttgcagact cgccccgcca agctccccac aatctcccga gcatgtcacc tccacggttc
    10021 cggtaacggc atcacgctca catgcgtcgc tttagcccgg actcgtacca cccgccccat
    10081 caaactcaca tccaccagca catcctcacc gtccaaggct cggacgatgc ccgtcatctg
    10141 cgcatatggg cccgaactca cctgcaccac gtcggccacc ttggccatga gtggttcaac
    10201 attgctaagg tcctcaaaga ggtcgctatc cagcaaagga agcgctcgca ccactccaga
    10261 cagccgcttg atcacgggaa cggccgttgg atcacagtga gcaatcacat agccctttgt
    10321 cggacgttcc acgtccgtgc accccaactc ctggagctgc aaggccacgt gtcgttcacg
    10381 gcctggcgtg acatcaaacg cccaccgtgc atgtaacgca ttctcagtgg agcacgcctc
    10441 tgtttgtctg tcgctcacag ttcctccttt tgaaaaccgc tccacctcac cccgcggact
    10501 catcgcagca agccacgggc tcgtctgatg cctgcgaggc gctccaatgc ccgatacacc
    10561 ttccagctgt agcccgcgat gttgcgactg ccgttgtaac ggcctacggc tgtccacgtt
    10621 tcttggtact cattgatctt ctgccgcaag acaaaggcag cccattggat attgatacaa
    10681 ggatcgagaa aatggactgg atcaattttg tactgccgca tgtgcacgct attgatctgt
    10741 gcaatcccca catcaaaatt cgcccccgcc ttccacagtt cagcaaccag atccgtcgct
    10801 tgcttgaaag aaagtggtcc tcgccgatcc cccttgccgt cccgattcac attcaccgca
    10861 tgcggatccc cttcactctc cactagaata atggcttcca atatttgcac ggtcaccgcg
    10921 tagcgattcg ccgccgccac aacacaactg tggttcacat accaaaaccg gtccggaggc
    10981 atctccgccg caacactgtc cggcaagaca ccgagacata ccaccgcaag acagaaccac
    11041 gtcgaacggc gacttttcgg tctggagaat gcacccagag gcccaccata cccccgtctt
    11101 aacaggtgcc tgtgtaggtg catatcttcg tcgccatttc cttcgtgatc acagccgggt
    11161 caatattcgc tgaaacattt ttgtaccatt ccgtgaaatc catcgcctgg aagttcaaag
    11221 cgctgaactg atcgaccgta aatccgtcgc actggggcgc atccgctgat ccccaggtca
    11281 tgcccaactg cacacggcct tgttcctgca ccatccgacc gagctcggat ttcatagtgc
    11341 agtagacctc ttcctttcgg aggcacacac tgccgaccag aaaggagcct ttatccacac
    11401 aatgtgtccc aattagatga gcctttcgat cgtgtttatc cctcgcaagc gcgatctcct
    11461 cctcagagca tttcccaatc aatttatcgg ggtccgaatc gcagcaattg attagtgatc
    11521 gcccgattgt gctgaaatca caggtgctcc ttttgcccgt gaagatacgc agacggtccg
    11581 gatcccaatc ttcttcgaca ttcttggtca tcgccagcca ggtggccgcc aacgccagat
    11641 tctcattggg tggcgtactg gtatccacgc aaaacggcac aggactgtct ggtttcccac
    11701 acttcgtgca ttgagtaatc ggttgcgtcg tcgaacattc cgacggtccc ggacacttgt
    11761 aggtgttctc gtacatcaag caatatcccg tccccggctg caccgatgcg cagcgagagc
    11821 tgacttgatt gcagcccttg tctcgatact cgccacaggt gtccccggcc gtggacgtga
    11881 catcccaggt ttgctcctct tgaaagcatc ccgttaaggg cgccacgctg tacgtatcaa
    11941 gaaaggttcc cgttaccatg accgcaatcg aatgcgactc gtaagtaaat gtaaatggaa
    12001 cttgccagct gagcgggccg gtgggactga aggcgcccat cgtaaacgtc atgggatacc
    12061 caaagcatga gcacttgcta ttgcagtagg ccggcgagcc atttccagag gccgtcgggt
    12121 tcgccggagt caacacgtaa ggcccggtct tcaccgagcc aacatttgtg cagccgtatt
    12181 tgtcttctcc ggcggaattg ccagtgacgt tcaagccgac cacccatcga gttccatccc
    12241 agataggaaa cgccgattcg atcgtaaaat tgcccatggc cattcccggc gcatggtcgt
    12301 gcgcgttgaa tccagaagtc cccccggtca gcacggtggc atacggttga gcacacttga
    12361 gatcccgcgc agctccatcg agacaggtcg tagtcaccag tgcggcgagg ccactggcgc
    12421 cagccgcttc tagatccttg cacccattga cctccgtctc agaggtatag ggagggctgg
    12481 gaattccagg aaccgtgcac gcgttcggta gcagggtggg gttgcgaatc ttgaggcagg
    12541 tctgccgtga gcccccaggc acttggcagg tccccgtgcc gccagcccct tcggtgtaac
    12601 tcgtgcacat attcaccacg gaacagctct ccgtcatgat ggaatcggtc ggcagcgtcg
    12661 ctgccgtcga ttgcgccgtc gtcaccgggc tactagtccg tagatcccac ccatctcggt
    12721 caatcctgct ctgttggatt ttcgagtaac gctcatcgga gctcgccgcc gcaccgccgg
    12781 ccgcgctcaa actgccagga tcatcgtaat acctctcttc agcagcccca gagcaccctg
    12841 ccgtgacaca acccggcgac cctgagtact gcggcacttt ggtcatgttc ggcgccagaa
    12901 tacctgcacc gactaatcct gaggacttct gcccctctac ctcacacttc gctttgaggt
    12961 acggatcgtc aatcccactg caatctgcgc gtacctccac aacaccacac accaaacaga
    13021 tcgcgaggca aatccctatg agcatgcgct tcatcacaga cagcctcccc ctatcgacgc
    13081 gtgcctaacc gagccgtatc caatgagggc accgtcatct cgtccatccc taccgcttct
    13141 cccagtgaat ttccgtccgg cggatgtcga gcggactggt cccttgcaac caagggggac
    13201 agttcaccgg ccaaaatcgt cccaagcgcg tcacgatcca taaactgaat cggcgttccc
    13261 cgcatgatga tttgtgccat accccacccc cagtatcgtg ccgtcacctg atgaatctga
    13321 atgtcgaaac caatgatcag gtcagctccc aacgccgcag cgctttgcgc aagcctattc
    13381 accatgctct gagtcgcatg gacgatcgga ctgtcatacg tcttcgcgcg ccctccccag
    13441 aaatcggtga atcggacaac ggcatcccgc caggaagcga tcccgacggt ctcgatctga
    13501 atcaatgcct gaaaataccg ataggtatgt ccgggaatga catccaccgt aagaattttc
    13561 attcgggcct atcgcttttc ggtatcagac ggtgacacct cggccttctt cggagcatct
    13621 gcgtcgatac tgatcgtcaa tgtgttcatg ggcgcggcca tcacttcccc atcgacagcc
    13681 ggaagaaggg gaggtccact tgaagctttt ccggacatcc cagaagctag aggaggaggc
    13741 accagttccg cgttttgccc attcccccca cggaagccat ccagaaatcg tcgtaaccat
    13801 cctttgtccg catcgggctt ggttcgaggc gattcgacct tagcctctat caccctctga
    13861 ggaacgggca tcggcacatc cggtttggca gggacagtgg tcgtcgccac tggacttgga
    13921 gcgaccgcca ggctcgtccc gctcggctca acacccgagc ccccagcgcc tacgctcccc
    13981 tccatattcg gactgccagc cttggcgaca ggttcagttt tgggagcact tcctgactta
    14041 ccctcaccca actgaacgcg cgttttctgg acgtcgggag caccccctct agcctgcaag
    14101 gacttcccaa gtttcccaat cgacagttca acacgttcga agcgattggc taaggcgacc
    14161 gtcgtttctg tgagggtttc gatcttcccc gcaatatttt gcaggcgcgc atcgactccg
    14221 gacagttgtt gcgcctgctg cgcaaactgc gcccgctggg actcgtactg attctgggtg
    14281 tctttcacca cccccttcac ggtcaggatg tccccctgca gtttttccgc ttgggccttt
    14341 agtcccatct gctcttgcgc aacatgccga tacacctggg atacattgtc gactcgctgg
    14401 gacagctcgt cgtgctcggt ctccatgact tccttcaccg cgagttgatg ctggtacttg
    14461 gtttccaaat cctgatattc accggtgctg acgcaaccgg acaacagtgc gagactaagg
    14521 gctgccatgc gttgccacat accgtgctcc tttcatacga gaggggtgac tacctccctt
    14581 ttccctattg gttcaaaaat gctttcaccg cgccccgcgg ctcatactct gtcaccgtca
    14641 cgcgagcgac atgcccgatg acatcgtgca aggccactac aagctcatcg acggcctgaa
    14701 aatgtttcac atacggagtg agatcttccg ctcggagacg aatgctattg agcgcgttat
    14761 ctagggcgcg aatcaccggc accagtctca ccgtgtctcc gacgcggggc gtaatgacac
    14821 gcgcgttgcg ccacgccgcc ttcacgctcc gttcttttag gtcgggatat gggtcttgag
    14881 ggagcaactc gaactccccc actccaagct ctcgcaagat cacctttgcc cgcgacaccc
    14941 attgctccac acgagccaac tcgttgcgca cttgctcaat ggagagctga gatcctgctg
    15001 cccgccgaag acgatgaatt accttgtcaa acatgtacag catggagaca caggccatcc
    15061 ccgcttttgt ttgtggtaaa aggagcaccg aattcggatc ttccagccgc cgcttgttct
    15121 cctccgcccg atcgggcctt cgtccattcg tcggagacgt gctccgaaga gactggccct
    15181 cgcgccgtac catccccagt tgtcgctctg cgtccacccc atgagccatg cctcacctcc
    15241 tcgattacgc ccgagctgtc ttccccagat ccgcttcgtc cacgagcacc agtccatagc
    15301 cggtcaattt gctggccacc gcagcattca tccccttgcg accaagaaac tgtgcgatcg
    15361 tgccccgttc aacaatgact cgcgcctgat gttcttcccg atacacgtcc actcgtcgca
    15421 ctgaccggct cggggccggc gctaatgcct cacgaatgta ggcttccacc tccgtgtgat
    15481 acttcacgag tgcgatcgtg cgggatgtgt aggtccggaa ctcgtacaac agaggcttgc
    15541 aggtcgcgat gacatcttcc cccgtgcgat aagtctcgac cgcaatcttc acaaacgacg
    15601 cggcttgagc cgtcgccaca cgcttgatgt gcacatggtc gtccgccaac accggagcca
    15661 gcactaactc cgcaatacgc cggaaatagt gcatcgatcg ctgcgacagc atcggaaact
    15721 cgtcaccgac cttgtacaca gctcccatca atgagtcgcg taccttgtac tgacggtcgg
    15781 catactgatg cggcaagatg gcatacctcc cgatggaatc cgcccacagc agatatgcgc
    15841 tgtgccccgg atccatccga atgacccgag ccattactaa gtctcccacc tgcaccattc
    15901 gtgactcgcc tgtgctatag tttttcgacg cgtatacgca cctgccacct gcgtgtagtt
    15961 cgagactcgg tctcacaact atgcccaaca ggtattaccg gatcactccc actccgcttc
    16021 tatcctcatc cagagggaag atccgctctc ttgcgtacct tgcttggctg tggtctagtc
    16081 ggacgatcag cgtgctcctc ctcgtgctcg tcaccttggt atcttgtgcc cccgtcttca
    16141 cgccgaccac tttgcagaac ttcaaagaat cgcgtgaaac cagctcaacg acggctgtag
    16201 aacgtcctcg attggcacta tcaccccaca gtgatggcct tggatggacg gtgagagtca
    16261 cccaaaccgt tgaggagaag aatcggatca agcgagaaga agtctgggga ggatatacct
    16321 atgaacgtcg gctcaatctt gtcacgatgg tgggaggggg actactctgt gctccaaatc
    16381 tcctgttgaa taccctcttt tccatgggaa caccaaacca gccgggatgg agttcaccat
    16441 ggcaatactg cctggccgcc gccggatatg acgtgcctgg ccgcgccgat acccgaactc
    16501 ggatcgctga tgacctcgat ccccaggttc agactgttac gcggataatt gtggacggtg
    16561 accttcggct cacttttgcc gccgatggac gggaccgcat tgggattgcc gtccctgtga
    16621 gtaaggattc tcaagggacg cacatccgcc ttcgatggct tgcaatggct ttacgggaac
    16681 agggcgtgaa tcctgccgac gtccctgacg ggaccgtcga gatttcctac agtccaactg
    16741 tcggcaaccc cggcccgacc acacagttgc cgatcgacgg gcacgtactc accgctatgc
    16801 tgcagcacga tctcgcagtg ctcccccaag atcgctggcc gaagcagcct cgcctcaggc
    16861 tggcggtcaa caatgactgg ggacacgaac agtcccagtt gatagtagac aggcttattg
    16921 gccgtatgaa ggagcgcggc cttccgctgg tcatccgaaa cgaacggtat cggaaactag
    16981 ttcctgtcca ggtccggcaa ctcgctccgt tctttaatga tgagcgacaa ccgtcagccg
    17041 gcatgggcac cggagcaaat ctgctcgtat ccatcgacat cgcctctgct tcagatcagg
    17101 ggctgctgct caccacgact cttgtctctg ttgagaccgc acaagtgctg gctcagtttg
    17161 tcctcgaatg tccgatcaac gccgtcgcgc caacggccga aactctggcg gcagttgtca
    17221 ccgttctcgt cctgccccat ggtcccactc tcacacgggg atggttcgtc acaccatgac
    17281 acgatatggc acattcctca tctgcttcct cgccaccagt gcggtcggct gttcgtctct
    17341 catactctcc aacaccccgg tgcagggaga aaatacctcg actcgagaaa tcgcgcgcac
    17401 tccgatcgat gtaccagagg ttacccttac accccatcac gatcagcaac gcctcaccat
    17461 tcgcgtgaca caacgctccc aaatcaccct cgagacggac acgccgataa aggtcgaggc
    17521 acgacgctat gtgttttcgc ctaccgctcc ccttgccggc gctgttcatt gtccggtact
    17581 gactgttgcc tatgtgattt cagccggaca cctaggctca caatctcttg ctcccgcctg
    17641 ccgacggctc ctgatgcagg aacctcttgc cggatcaccg cgggttgcca ccgaaatcga
    17701 acaccgctac gagacacaca ccacaacaga gcccgtgcgc tacgcctcac tgagagtggc
    17761 atgggacgat gcgatacatc ccccactggt ctatgacgtg aatcccgatg gagaggtgtc
    17821 cattcctcaa cacgcactcc gttcggggac ggagcgcgac ccgtcgacgc ttcccacact
    17881 gtctatcctc gtcgacaatc gcgtcctctg gactcatcgc cttaccccta gcgagatgac
    17941 accaagtggc ccattgccag acgaaccgca cacggcaggt acgagctggc caccgatcgt
    18001 cgcactcgtc ctcgaaccgc tcgatggcgc tgcaccacct acgttgagag catggggacg
    18061 tttggtgaca caggccttat tggaacgcca catctgtgtg gtcgcaccaa taaccttcgc
    18121 taaagactta caggatgagc tgctactcca ccaatccggg cggatcagtg accagacggc
    18181 tgttcacttt ggcaaatgga tacccgccac tgtggcgctc accatcagaa aaggggccgc
    18241 caatcaggga atagctattc tagaattcgt ggatattcag agggcggaac gaatcggata
    18301 cgacgtccta cggtatagta gcgactggaa agaggatgcg cagatggtga aggcctatat
    18361 gcaaacgatc ttccccaaaa gatatcaatc ctgctcatag caatctcact tcgcatcctg
    18421 tacctcaagc acgcaatcac catacacagt gatcagaagt gaacctggat acatcgtgat
    18481 ggcttgttta tcggatccac tcttcgacat atccccaaca gatgtctcaa tcctagtgtt
    18541 taagagcgca cgcccttttg tcatatcgcg acaatagctc acaagttcat ccaacaccgc
    18601 taaggcgttg ccctcgaaag atggcctaaa gcgttttgaa agaacggcga tcgttgaatg
    18661 gcctggcacc ttgccaggaa ttgctgatcc cacaaatgca cctagccagc tatctgttct
    18721 agtttctgct aaagaatggt gcgctgagca caaaaataga agacttaggc ttagaaaaat
    18781 acgcgctcga agcagccgca ttgcggcctc cctagatatg gctggtaatc gtagtcagtt
    18841 acttgttgct gccggtattc acaccttgtg aagacggttt tgaaatcttc aacagcgact
    18901 cttggccttc ctgccacctc ggcatagtcc caaacgcact cgacgcttct tctcccatca
    18961 tgccaagggc cgatgtgcgc accgcattat aagcggcatt ggcagcaaag ttctgtaatt
    19021 cttcgggaga catgctttgt gcacgcccgg tcttttccaa atgttcgaca gaactgcggg
    19081 caaacgagat agcattatcc atcactccgg aatattcttt ccaccaggcg cccatatttt
    19141 gatgcgagtc caaaagaccg ctcggtcccc cacctccacc agggccattc ggcacaggtc
    19201 cactacctgg ctgaaccggt gttcctccaa cccctccccc accaaatttc cccattcgtc
    19261 ctttgaggat caaatatcgc gaagccccat ccattccgat ccgaccaaat tcaagcccat
    19321 aagcaatcgc ctcgtccgtc tgcgtagggt ttgtaatatc ggtgcctttg gccgcaaacg
    19381 ccatttgtgt aatcgtcaga ttcgccccac tgtcatgatg aaattgcaca cgattaaagt
    19441 catgaaaatc ggagcctttg tgaccggacg aactcagcac attcccgcta ccgtcaaata
    19501 catcgttaac cgcaaatcca tgtgccgcat actcattctt cgatagctcc ttcgtatttg
    19561 caatcacgct cgccccatcg ggcgactgtg aatactccac tacttgaaag tgttcttgcc
    19621 cttgtgcatc agtacggtac tgaccgattt tccctgtttg caagtccgtt acttctgacg
    19681 caattaactg gccagactga ggactatagt agaacgtccc ctgcagctct ttggtctgcc
    19741 cgggatgcat agcatcaggc acggtgaaaa tgccggattt acgctcattg ttatagtact
    19801 gtgagtccaa tccgcccaac accgaatgat acaagacttc gcgctggccg ccatcatcct
    19861 tcataggacc aaggacttgt gtgtctatcg ctccgtgttt cgccgccacg accttcaccg
    19921 tctgatcaat agagccgctc atagccccgc tgtttggatc ctccagtgac ctggcaattc
    19981 cccacacctc gttgccttct ccatctttca taaaagtgga ataggtgttg tcggtcacat
    20041 tggtaatcgt cccgcccagt agcttgcgtt cgccagtctc actactggcc gggccgtagt
    20101 agagattggc gtacacatcc atatcgcgat gcccgtcatt gatccgacgc cagcctgact
    20161 ctttatcgat cgactgtacc tcgttgttgt acccacgcag ggactgctcg aactcgacgg
    20221 tcatttggcg cgatcccctc atctgatcgc tatacacact caccccgtcc aggtttgtgg
    20281 atgatctttt gaaggcgtcc ccctgaggat taacggttac tccctgaaaa tttgataggg
    20341 agtgagttcc tccaggagca ctatatgtct cctgagcgcc gatagattga agatctccag
    20401 aatcagttcg ttcaataaca tctcggaact cgtttcctga ggctccttta aacgagacga
    20461 gactaccgtt atcaatcagc cctgtgacat cgcgggccgg cgcccccggt tgttgcgcat
    20521 acccatgaaa catctcaact tgatgtccat cttgtcccat cacatttaac tgcgagaact
    20581 gcccagacaa atgagtgcca tccaccagat tacgttcccc ggaaatactc ttggtacctc
    20641 cctcccgccc gtcaaaaaag ctcacctggc cgtcgtataa cagcgcattg gcttgaaacg
    20701 ccggagaacc tggcttgcta ttgtcgatgg cagagccgaa gaaggctgtc ggcactcctg
    20761 gttgattggc ttccccatga ctcagagccg tgaacgtgcc gctcagggtg taggaaccga
    20821 ccgtgtgggt gtcgtgggca acaccgctcg tgcctgactc aaaatgttcc gtggcttcca
    20881 ccaagaactg tcgtcctgat gcatcggtgc cca
//
LOCUS       DAORRQ010000068        20671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_156_length_20671_cov_14.255724, whole genome shotgun sequence.
ACCESSION   DAORRQ010000068 DAORRQ010000000
VERSION     DAORRQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20671
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156_length_20671_cov_14.255724"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2023
                     /locus_tag="PK224_16835"
     CDS             <1..2023
                     /locus_tag="PK224_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17464.1"
                     /translation="FWHDHALGRITWGHGGFGTMIVEPVGSTYHNPKTGAPIRSGPLA
                     DIRTAEPVGYGVNGSFRELLVQLNDSVPHTINIVTAGNPPGQPVEVALEAGKTISFPI
                     PDHIKMTPMPFLNGGTHTTGGSLNFRAEPISGRMAANPDMSKLFSSAVHGDPYTPMVR
                     AYLGDTVVFRLLQTMTNESMVWTLSGHTYLTERYAGDANRKNSIHVGIAERYDLVVPQ
                     AGGPRLQAGDYIHFNGRSSKFSEGAWGILRVLDKDVSDLQKLPAGYSGRNEIPKAPAV
                     CPADAPVKSFNVSSIDFPSMKLNPKAPDAIEVDFERTIQMVNPEARIYVLDEDVATVA
                     SGVQPMPLTLRANVGDCLKVKLTNKMKQGRASFSAIGLAFDPKDSLGANVGNNPGEQT
                     VAPGESRVYTHYADPFIGETQSLVWDWGNVMTNPRNGLYGGIVIGPKGATYRDPKTGA
                     DISTKNSWVADVIVDRTIQGYEHRQNYRDVALFFQDEDNIIGTSFMPYVQNTAGLTAV
                     NYRSEPYKFRESNGCTLGKVFQPCSVDKPESIATPLIEAHAGDPVRIHVFGASNEQNG
                     MFSVEKHEWPIEPFMRGADQISVVEFSASETLDAFIPAAGGSFRLPGDYVWSNQRLPY
                     AQSGQWGLMKVLPHDDQRILPLSQQAPSIKRAEVESGTPTVSRMSSPLR"
     gene            complement(2223..2984)
                     /locus_tag="PK224_16840"
     CDS             complement(2223..2984)
                     /locus_tag="PK224_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRB17465.1"
                     /translation="MNPGSNRLLLSISLALTACTILSPLEVLAAGPTGEPLTRQAFEQ
                     MIEQYIRSHPEVIEQSLQSLENKRAAEEQERQRAALATHQQELLNDPASPISGNPAGD
                     VTVVEFFDYRCGYCKKAASALTQLQQSDARVRVVYKDFPILGETSELAAKAALASNLQ
                     GKHRAFHEALLAAKDDLTKEQLFHIAQDTGLDVTRLDRDLSRPEWQSTIERNRILAKT
                     LGISGTPAFIVGNDLVPGAVDLKTLQELVAHKRKQ"
     gene            complement(3134..3427)
                     /locus_tag="PK224_16845"
     CDS             complement(3134..3427)
                     /locus_tag="PK224_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17466.1"
                     /translation="MGLHILAILTLLLSTPIISGFAAGPTMEVALLGGTILSIDQDTL
                     TLTIRMPSGESQALPVINRRLLQGLSIGDHVSFELDEAHRLIKITKLPIDPAN"
     gene            3586..4797
                     /locus_tag="PK224_16850"
     CDS             3586..4797
                     /locus_tag="PK224_16850"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRB17467.1"
                     /translation="MSETGNGSPAVLTLNAGSSSVKWALFRMDSLPVRTATGKIERIG
                     LRDGIVTVTDVATGHNERRIEQVSDHAASVRLLIDQLAHSGKGLSVQAIGHRVVHGGS
                     RYADPQVVSAEVMEELRRLSPYDPEHLPAEITFIEAFGTEYPQVPQVACFDTGFHQHL
                     PPVARLLAIPRRYETLGIRRYGFHGLSYAFLMEELERVAGRDAARGKVILAHLGNGAS
                     LAAVRDGVSIDTSMGFTPASGLPMSTRSGDLDPGLVSYLARTEGMTTEQFHHMVNAQS
                     GLLGLSEISPDLRDLLAQEGRDARAAEAVALFCYQGKKWIGAYAAALGGLEQLVFSGG
                     IGEHAAVVRARMCEGLEFLGIHLDREQNEANAAVISSPASRVTVRVIRTDEESQIAQS
                     VCRLLALTVGR"
     gene            4871..7246
                     /locus_tag="PK224_16855"
     CDS             4871..7246
                     /locus_tag="PK224_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HRB17468.1"
                     /translation="MNLTTQANQSLSPEMAQRIHAYWRAANYLSVGQIYLFDNPLLKQ
                     PLKREHIKPRLLGHWGTTPGLNFIYAHLNRIIKAQDLPVLYIAGPGHGGPGLVANVYL
                     EGTYSEVYPNISQDESGLQRLFKQFSFPGGIPSHVAPETPGSIHEGGELGYSLAHAYG
                     AAFDHPELLVACVIGDGEAETGPLAGSWHGNKFLNPVRDGAVLPILHLNGYKIAGPTV
                     LARMPPDELESLLVGYGHQPFFVEGDDPATMHQLMASTLDEIVTTIRGIQQEARSKGF
                     TGRPRWPMIVLRTPKGWTGPKEVDGKPTEGTFRSHQVPMGEMDKPGHVELLEAWLKSY
                     RPEELFDEAGKLMPELAELAPTGERRMGAIACANGGGLLRDLQMPDFRQYAVPVVKPG
                     VEDAEATRVQGLFLRDILKMNPHNFRLFGPDETASNRWGGVFEVTDRCSTEQILPTDE
                     HVAHDGRVMEILSEHLCQGWLEGYLLTGRHGFLNCYEAFIHIVDSMFNQHAKWLKTSR
                     EIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVVNKNAQVIRVYLPPDANTLL
                     SVTDHCLRSRDYVNVVVAGKQPAPQWLDMDAAVLHCTAGIGIWEWASNDRGSEPDVVM
                     ACCGDVPTMETLAAVEILRIAQPNLKVRVVNVVDLMKLQPASEHPHGMSDKEFDALFT
                     TDKPIIFAFHGYPWLIHRLTYRRTNHDNLHVRGYKEEGTTTTPFDMVVLNDLDRFHLV
                     SDVVDRVPQLGARAAYLKQEMRDKRMLHKQYIETYGTDMPEIRNWKWSGKR"
     assembly_gap    7296..7339
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7340..8103
                     /locus_tag="PK224_16860"
     CDS             <7340..8103
                     /locus_tag="PK224_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HRB17469.1"
                     /translation="RPNENSLAGREDRTPEQGFMNSPGKDMAPPTVVVLFDVDNTLLD
                     NDRITADLTQYLDREVGPAHEREYWRIFDELREELGYADYLGALQRYRLAYPHDSHLL
                     AVSHFLVNYPFADRLFPQALDVVRHARQWGRTVILTDGDAVFQPRKIEQSGILSVVEE
                     QVLVYVHKEQELEDVRQRYPADHYVLIDDKLRILSAVKAQWGARVTTVFPRQGHYAND
                     PDALRTFPSPDLSIDCIGDLLTIDQSRLFPAAGHR"
     gene            8186..9247
                     /locus_tag="PK224_16865"
     CDS             8186..9247
                     /locus_tag="PK224_16865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRB17470.1"
                     /translation="MTPKKPDTSIWSTSLDRRQLLKGFGVAGSVMALGGPGRLAELLA
                     ADTPSSGTSTGDIPRRALGKTGVQVSALCFGGAHWGRLKDESEAFRIIHEAIDAGVNF
                     LDNAWEYHGGRSEELMGKALQGKRQQVVLMTKVCSHGRDKKVALQQLDESLRRLKTDY
                     LDLWQIHEVVYEDDPDLHFAANGATEALLEAKQQGKVRFIGFTGHKHPQIHLKMLGHD
                     FPFDACQMPLNVFDSTYRSFEREVLPVLNQRGIAALGMKSLSGNAEPIKQGIVTPEEA
                     IRYVLSLPIASLVSGIDSPQVLKQNLDIARRFTPMTVAEMDGLRTRVAQYAMDGRYEL
                     FKSTNRYDGGIGRAQHGIS"
     gene            complement(9377..10891)
                     /locus_tag="PK224_16870"
     CDS             complement(9377..10891)
                     /locus_tag="PK224_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HRB17471.1"
                     /translation="MLRWRSHSLWFNLPFLVCAWLSLLSSAGLAQSSGAHEAGQGREP
                     GLIGPVNHRAETQEPSTLPKDRSGSLDWENRRGRSYLIPAAEVLTYLFLLNQYDRHFT
                     EPKDVYRTTGTTIREHLTDSKWVFDNDQFSVNQFLHPYGGSVYFGLARSAGLSFWESS
                     LYSVAGSFLWEIGGERTSPSINDMITTPIGGTFLGEPLFRMANLLLETGDGPPGFWRE
                     LGAAIISPPTGFNRLVFGNRFDSVYPSHRPATFLRLEAGGTLTSSSRNVSSSVNEHGA
                     VGELTFTYGLPGKSGYGYTRPFDYFDFHVSAATANTLETITSRGLLLGKAYTSGDTTR
                     GIWGLFGSYDYISPQVFRVSSTALSLGTVWQSWLSEDIALQGTLLGGAGYGAAGSIQR
                     TEERDYHYGTTPQALVALRLIYANRLMFDVTGREYYVSNFLSPERHGQENIIRVESAL
                     TLRVFDRHGVALRYTLSHRDAHYPSVEYRNQTVGAVSLMYVLLGNSGFGAVEWR"
     gene            complement(11010..12290)
                     /locus_tag="PK224_16875"
     CDS             complement(11010..12290)
                     /locus_tag="PK224_16875"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRB17472.1"
                     /translation="MSLSHSGLTLSVLFLLGVSTHAITAPDSRASIYTYTDEAGVQTF
                     TNQLESVPEQYRNRLTSQELDGPPAASSAPPRAVAESPTRSADSRIVTASGEYRLGDH
                     DTRTDGARLAVEAAKRDALEQVATYLERVTEVRDMNLTRDDIRSYTAGIVTVLEETIT
                     TRVENESIVIRAELTAEIDPHEVAQAIAALRENDSAMRELTVLRAETDRLQEQLDATN
                     RALAAAPSAEEVQQLSRQRDDMLNDLQANALVSQAWTSWAYPTLGFYSYPWIAGPGIN
                     GLLLQAQRLSPRHRHLAQAQQTIAAQNGALVPAPAHAFNPTLRRSLLVPSPSMQSPHA
                     PPLLNQQGLPAKVGDIVTIPTPRSIPPVMHRSAPQPQPYQLHPSHFWRPSPPNIPTAP
                     SITQQTPSINRPLGGPSGHGSSGQSRGNSGGRGR"
     gene            12497..13165
                     /locus_tag="PK224_16880"
     CDS             12497..13165
                     /locus_tag="PK224_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17473.1"
                     /translation="MLLVRQIASILFGFVPLVLIAVGVASVLWEVQAHDPSMFSVQGE
                     KHLAHAFMFAGAGCIGLFGCHRLWRANGSWRWVLVPAAVMIFAVVFPNMERWPHGTPV
                     ERASFSTLQRLSAFAGSATQLAREQGEFVCDRSATLSASSLFARQGQALSYVIHCVPD
                     ATGPTLGTPPERPGTLVFAVSPDRTQAWFTATVLTHSTGRQASWLTRQGQPFVIAAQL
                     QSKS"
     gene            complement(13301..14143)
                     /locus_tag="PK224_16885"
     CDS             complement(13301..14143)
                     /locus_tag="PK224_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17474.1"
                     /translation="MTSHTHGKQSNVLANDFPHNLNRRSADRAWILLIFCVIATMFSA
                     LSSVADAGPPELPFSLEFETGAVWQSRNQIQIPDSSAGTRFALTDIQGHGPEAQRRVE
                     LTWNMNHRHSLRFVYAPLGFSGTGAFGQQVRFAGSTFAPGSPIDSEYKFDSYRLTYRY
                     LVHETERWRWRIGATAFIRDARVELRQQGVLASDSNVGFVPLLSSSLEYDIAPRWTAL
                     LDFDGLVSTQGRAIDAAVKIRYDITDHWFMSAGYRVFEGGVDNERYAFGWYNFALVSV
                     GLRF"
     gene            complement(14097..14693)
                     /locus_tag="PK224_16890"
     CDS             complement(14097..14693)
                     /locus_tag="PK224_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB17475.1"
                     /translation="MISVMDACPTLHTERLILRQFQASDADTVHRLAGVKEVAAGTLL
                     PHPYDLEAAAKWIAQQQESFAAGNAITFAIVLAEEERLIGSIGMDIASEHQLARLSYW
                     LGTNYWNQGYCTEAVRAVLDYGFTRLSLHRIYAPHFHNNPASGRVLRKVGMTYEGRMR
                     EHYIRFGRFVDVEIYGILREEFLNDITHSREAVERPRE"
     gene            14879..15367
                     /locus_tag="PK224_16895"
     CDS             14879..15367
                     /locus_tag="PK224_16895"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRB17476.1"
                     /translation="MTAFNPLAALRSPRETLDGYIILPRLIDKVRVQARGELPAAYQR
                     NLLHRGGTLDGRFLAFTGLEGEALRAVILSCETDEPVAQWVAQHAARHSADEKQRWAA
                     EVEGYRPTGRVLLYLQSTVPELAAQIDCALVSLLDLIDMDEGRLAVPARGGSADVEER
                     SA"
     gene            15345..15980
                     /locus_tag="PK224_16900"
     CDS             15345..15980
                     /locus_tag="PK224_16900"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRB17477.1"
                     /translation="MLRKEAPEWYAPLGDDFGPEQMRGEIAVAQRYKELSKTHIEFIT
                     TQKLFFVGTATAESRVNVSPKGMDSFRVLGPARVAWLNVTGSGNESSAHVQHDPRMTI
                     MFCAFEGQPLVLRLYGRAKVVHRGDSEWQEFFGLFPPLPGARQIFDVTLDLVQTSCGM
                     AVPYLSYLGDRESLSAWAEKKGDEGLRRYREEKNQLSLDGIPTHIVDKEGH"
     gene            16111..16311
                     /locus_tag="PK224_16905"
     CDS             16111..16311
                     /locus_tag="PK224_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17478.1"
                     /translation="MAQVLIAIVGLGAAVTTYCLMRFPHMRRERFRHYGAFSIAVSVL
                     GALAVLLFCLALLILQNAWRVG"
     gene            16619..19948
                     /locus_tag="PK224_16910"
     CDS             16619..19948
                     /locus_tag="PK224_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17479.1"
                     /translation="MADYFLRDVETIMGLVRTGHSLQLLPRREAEAQADCLEFPRLSF
                     KPPPHPVSIDRVDWVFAGLLALSVAILCLLIARSMPSFLFQSMDFWFEADTLREISNM
                     SRVHDDHYRTSVHPLFSLLTFIPVYIAKHALTTSPLRAVLLITGVVGGLWAGTLYLLF
                     RLLGCRRLDASVFTLLGLCSASALFWLPVPNSYSWGSLSVMLALVLLLFAEQRPFWAT
                     AYVIASALTLSFTVTNWMAGLLVTLVRWPWKQAVQLSVNALCVVVLLWGVQKFIFPTA
                     EFFIGHREEAEFINHPQSGGAANVLSSLVFHTVLAPDVRFMKDDAYTQAKSDSFRLSE
                     RLTFQFSRPGSTGPLGLLAVGLWSALLLNGLWRLVTLDHHLRFRLVLASLLGFETLLH
                     LLYGEETFVYSLNFLPLLLAVAALGAISPGRRVVVSLAALLALCAGLNNWHQFQEATR
                     SATQFTPQRELMTTMMQQDPDRPWPRSVGHVPLALPGTPEGGTAYHEPGGDFSPHVPS
                     FGVSIWLCDANGLPVVTSQTVPLSDIQQRFAPSTHPNIPAIVTKTPYYQATWSRLDAT
                     HWELRFKNYTNHAPAILIRSVGPAGGPVTALDWDGEQIGINHRWTVKATPAPGAITLG
                     DENTSDWMTAESSAKSWSGASGWGYARMTFAPASTRKDDEVRVVLSDLQPPVEIQTHY
                     THPSKRARLALPEPRLQASMDAQITHLMMSLVDDETRPGDPTFYYRAWHRQGSYITAA
                     LARAGDPHVSRVLSQFLASHDFAGGSGPEADAPGLAIWALTESAAYIADPVHDQWLWP
                     HILRKAQRIEGMLTARAPLVEPYWIPSPHDFNHGRQTRTALLAQPARDGLIVGRVGED
                     WPLFYVNAVSYRGLLAAADFAERLGKRQQAARWRQHARGLEASWQRQFPTGSPDSVTY
                     PVSLRALAGSATRRTQLGQPMTTYRPPAVSPTAATQLTQAHRALRLGRPEAVWATLHQ
                     LWSHQASPGLYTWDAPRPTQDEVADGWQYARGWHNETAVSPDYETAALLLLLQQDMLA
                     YLDEAAAEPTVVLGAGITPAWLSQPMAVSNLALPGGSITWQWDGQKMRVTLRGPVRKI
                     QLGSAFPPGTELAVVQKPLRP"
     gene            complement(19984..>20338)
                     /locus_tag="PK224_16915"
     CDS             complement(19984..>20338)
                     /locus_tag="PK224_16915"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRB17480.1"
                     /translation="EGVLGFEEFLTRVEVDRFDRIIKTPELFEKFLPYAMALGVEKNW
                     AGAFESIVTTAPAWYQGAELAQFNTRGFTSRIGDMAARTGPTMTSAPRSSGGSGFGGG
                     GSSGGGFGGGGGRGF"
     assembly_gap    20339..20392
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20393..>20671)
                     /locus_tag="PK224_16920"
     CDS             complement(<20393..>20671)
                     /locus_tag="PK224_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17481.1"
                     /translation="YERLLLEALFKDSTTDDVSLSSLENRFYRSLPPIQDAIFESLQK
                     RKYYTQRPDRVKQGYLIGGIVLGMLLTFALSALADRWGMAPLTFLGAGA"
BASE COUNT         3854 a         5948 c         6524 g         4247 t
ORIGIN      
        1 gttctggcac gaccatgcgt taggccggat tacctggggc catggcgggt tcggcacgat
       61 gatcgttgag ccggtcggtt cgacgtatca caatcccaag accggggcac cgattcgaag
      121 cggtccgctg gcggatatcc gcaccgcgga gccggtcggc tatggtgtga acggaagctt
      181 ccgtgaactc ctggtgcagt tgaacgatag tgtgccgcat acgatcaaca tcgtgacggc
      241 cggcaatcct ccgggtcagc cggtcgaggt ggccctggaa gcgggtaaga ccatttcctt
      301 cccgattccg gaccacatca agatgacgcc gatgcccttc ctgaacggcg gtacgcatac
      361 gacgggcggc agcctgaatt tcagggcgga gcctatttcc ggccgtatgg cggccaatcc
      421 ggatatgtcg aaactcttca gcagcgcggt gcacggcgat ccgtacacgc cgatggtccg
      481 cgcgtacctg ggcgatacgg tcgtcttccg gttgttgcag accatgacca acgaaagcat
      541 ggtctggacg ctgtccggac atacctatct gactgagcgg tatgccggtg acgcgaatcg
      601 gaagaactcg atccatgtcg gcattgccga gcgatatgac ttggtggtgc cgcaggcggg
      661 cggaccgaga ctgcaggcag gggactacat ccacttcaac ggacgttcgt ccaagttctc
      721 ggaaggtgcc tggggcatct tgcgggtcct cgataaggat gtgtcggatc tgcagaaatt
      781 gccggccggg tacagcggcc ggaatgaaat tcccaaggcg ccggccgtgt gtcctgccga
      841 tgcgccggtg aagagtttca acgtctcatc gatcgatttc ccgtcgatga agttgaatcc
      901 gaaggcaccg gatgccatcg aggtcgattt cgagcggacc atccagatgg tcaatcccga
      961 ggccaggatt tatgtcctgg acgaggatgt ggcaacggtg gcttccggtg tgcagccgat
     1021 gccgttgacg ttgcgcgcca acgtcgggga ctgtctcaag gtcaagttga ccaacaagat
     1081 gaagcaggga cgggcctcgt tctcggccat cgggttggca tttgatccga aggattcgtt
     1141 gggtgccaac gtcggcaaca atccgggcga gcaaacggtc gccccgggtg aaagccgcgt
     1201 ctacacgcac tacgccgatc cgttcatcgg ggagacacag tccctggtgt gggattgggg
     1261 gaacgtgatg accaatccgc gtaatggcct ctacggcggc atcgtgatcg gcccgaaggg
     1321 ggccacgtat cgcgatccga agaccggcgc ggatatttcc acgaagaaca gctgggtcgc
     1381 cgatgtcatt gtggatcgga cgattcaggg gtatgagcat cgccagaact accgcgatgt
     1441 ggccctgttc ttccaggatg aagacaacat catcgggacg agcttcatgc cctatgtgca
     1501 gaacacggcg ggtctgacgg ccgtcaacta ccgctcggaa ccctataagt tccgggagtc
     1561 taacggctgt acgttgggca aggtcttcca gccttgctcg gtagataaac ccgagagcat
     1621 tgcgacgccg ctcatcgagg cgcatgccgg agatccggtg cgcatccatg tgttcggtgc
     1681 gagcaatgag cagaatggca tgttcagcgt cgagaagcat gagtggccga tcgagccctt
     1741 catgcgcggt gccgaccaga tcagcgtcgt cgagttctcc gcgtcggaga ctctcgatgc
     1801 cttcatcccg gcggccggcg gaagcttccg cctcccgggt gattacgtct ggagcaatca
     1861 gcggttgccc tatgcccaat cggggcagtg gggattgatg aaggtcttgc cgcatgacga
     1921 tcaacggatc ctcccgttga gtcagcaggc gccttccatc aagcgggcgg aagtcgagtc
     1981 tggaacaccc actgtctcca gaatgtcgag tccgctccga tagtggtgag gggggcggca
     2041 tcaccctccg tcagtgccgc cacaaggatg cagtgccggt cctaatcacc aagactgcgg
     2101 gagagcccag gtgctctccc gcagttgcgt ttcgggtttg agcggtagaa tggtcccgtc
     2161 tgctttcggt aggtcttctc tcttcccgtc acatgtcaga caaacaggca gggcgcaggc
     2221 aatcactgct tgcgcttgtg tgcgaccaat tcttgcagcg ttttgagatc cacggcgccc
     2281 ggtacgagat cgttgccgac gatgaacgcc ggggtgccgc tgatgccgag ggttttggcc
     2341 aggatacggt tgcgctcgat ggtcgattgc cactctggcc tgctcaagtc ccgatcgagc
     2401 cgcgtcacat ccaatccggt atcttgcgca atgtggaaga gttgctcttt ggtgagatcg
     2461 tctttcgccg ccagcagagc ctcatgaaat gcccggtgtt taccctgcag gttcgaggcg
     2521 agtgccgcct tggccgccaa ttctgatgtc tctccaagaa tcgggaagtc tttgtatacg
     2581 acacggactc gggcgtcgct ctgttgcagt tgggtcaagg ccgatgccgc ttttttgcaa
     2641 tagccacagc gatagtcgaa gaactcgacc accgtcacgt cgccggccgg atttccactg
     2701 atcgggctag ccgggtcgtt gagcagctct tgctgatgtg tcgcgagggc ggccctctgc
     2761 cgttcttgtt cctctgccgc ccgcttgttt tccaacgact gcaacgattg ctcgatgact
     2821 tccggatggc tccgaatata ctgctcgatc atctgctcaa aggcctgtct ggtgagcggc
     2881 tcgccggtcg gtccggctgc caggacctcg agaggcgaga ggatggtgca agcggtgagt
     2941 gcgaggctga tggaaaggag aagtcggttg ctgcccgggt tcattctgcc tccgtttcgg
     3001 atgaatgtga tggggtgtcg ctcgatgcga gaacgcgcgg cattatacaa gaatccgttg
     3061 tctgagaaac tggtggcgcg cgcaacgaga ttcttgaacg tcgtagggcg ggaggcgcag
     3121 tggttgactc cggtcagttg gccgggtcga tcggtaattt ggtgatcttg atcaaccggt
     3181 gagcctcatc cagttcaaag cttacatggt cgccgatgct gaggccttgc aggagacgac
     3241 ggttgatgac cgggagggct tgtgattccc cggatggcat tcggatggtg agagtcaggg
     3301 tgtcctgatc gatcgagaga atcgtaccgc ccaggagggc aacctccatc gtgggaccgg
     3361 cggcgaaacc ggagatgatc ggagtactga ggagtaacgt gagtatggcc aggatgtgaa
     3421 ggcccataac ccatgatagc cgatgccgat acctgcccgg aactagcact cttccctggt
     3481 ttgcgcgggc agaggaccta caacgcaagc aaatggcgct ttggatttta cggttgcgct
     3541 gattccctag ccgcactatc ttgaagcgta gaagaaaggc cagggatgtc tgaaaccgga
     3601 aacggatcac ctgcagtcct caccctcaac gctggatcgt cgagcgtaaa gtgggccttg
     3661 ttccgcatgg attcgcttcc ggtccgcacg gcgaccggaa agatcgaacg gatcggactt
     3721 cgggatggga tcgtcaccgt tacggatgtt gccacagggc acaacgagcg gcgaatcgag
     3781 caggtgtcgg atcatgcggc ctcggtgcgg ttgctcatcg accagctcgc gcacagcggg
     3841 aagggattgt ccgttcaggc catcggtcat cgagtcgtgc acggaggcag ccgttacgca
     3901 gacccgcagg tggtctccgc tgaggtcatg gaggagttgc ggcggctgag tccctacgat
     3961 ccggaacatc ttccggccga aattacgttc atcgaagcat tcggcaccga atatccgcaa
     4021 gtgccgcagg tggcctgctt tgataccggg ttccatcaac atctgccgcc ggtggcgcgt
     4081 ctcctggcca tcccccgtcg ctatgaaaca ctgggcatca gacggtacgg gtttcatggc
     4141 ctctcgtatg cgtttctgat ggaggaattg gaacgggtgg ccggccgtga cgccgcgcgc
     4201 ggtaaggtga ttctcgccca tcttggaaat ggggccagtc tggcagcggt gcgggacggt
     4261 gtcagtatcg atacgagcat gggctttact ccggcttccg gcctgccgat gagcaccagg
     4321 tccggtgatc tggacccggg cctggtgtcc tatctggcca ggaccgaagg catgacgacg
     4381 gaacagtttc accacatggt caatgcgcaa tccggtttgc tgggcctgtc ggagatcagc
     4441 ccggaccttc gcgatctgtt ggcgcaggag ggccgcgatg cccgcgccgc tgaggcggtg
     4501 gccctgttct gctaccaggg gaagaagtgg atcggtgcct atgcggccgc gctgggcggg
     4561 ctggaacagt tggtcttcag cggcggcatc ggtgaacatg cggcggtcgt gcgggcgcgt
     4621 atgtgcgagg gcctggagtt tctcggcatc catctggatc gggagcagaa cgaggccaat
     4681 gccgccgtga tctccagccc ggcgagtcgc gtgaccgtgc gggtgatccg caccgacgaa
     4741 gaaagtcaga ttgctcaatc ggtctgccgg ttgctggcat tgactgtggg ccgttgacga
     4801 tgcaggacgg tcgcccatgc ccgtcgcggg cgcgatgtca cggacaatcg aaacctgaga
     4861 aagggaaccc atgaatctga cgacgcaggc gaatcagtcg ctgagccccg agatggcaca
     4921 acggatccat gcctactggc gagcggcaaa ctatctctcg gtcgggcaga tttatttgtt
     4981 cgacaatccg ctgctgaaac agccgttgaa acgggagcat atcaagccgc gcctgctcgg
     5041 ccattggggc acgacgccgg ggctgaattt tatctatgcc catctcaacc gcatcatcaa
     5101 agcccaggat ctcccggtgt tgtacatcgc aggtcccgga cacggcggtc ccggcctggt
     5161 tgcgaacgtg tacctcgaag gaacctacag cgaggtctat ccgaacatct cgcaggacga
     5221 atcaggctta cagcgcctgt tcaaacagtt ttctttcccc ggaggtatcc cgagccatgt
     5281 cgcgccggaa acgcccggtt ctatccacga gggcggtgaa ctgggctatt ccctggccca
     5341 tgcctatggg gcggccttcg accaccccga attgctggtg gcttgtgtga tcggggacgg
     5401 agaagcagaa accggtccgc tcgcggggag ttggcatggg aacaagtttt tgaatcccgt
     5461 gcgggacggg gcggtgctgc cgattctgca tttgaacggg tacaagatcg ccggcccgac
     5521 cgtgctggca cgtatgccgc cggacgagct tgaatctctg ctggtcgggt atggtcatca
     5581 gccgtttttc gtcgaaggtg atgatcctgc gacgatgcat cagctgatgg cgtccacgct
     5641 ggacgaaatt gtcacgacga tccggggcat tcagcaggag gcccggtcca agggatttac
     5701 cggccgtccg cgctggccca tgatcgtctt gcgtaccccg aagggctgga ccgggccgaa
     5761 ggaagtggac ggcaaaccga cggagggcac gttccgttcc catcaggtgc cgatgggcga
     5821 gatggacaag cccggacatg tcgagttgct ggaagcatgg ctgaagagtt atcggccgga
     5881 ggagttgttc gacgaagcgg ggaagttaat gccggagctg gccgagctgg cgccgaccgg
     5941 cgagcgacgc atgggggcta ttgcttgcgc gaacgggggc ggccttctcc gcgatcttca
     6001 gatgccggat ttccgacagt acgcggtgcc ggtggtgaag cccggcgtcg aggatgccga
     6061 agcgacccgg gtgcagggac tgtttctgcg cgatatcctc aagatgaacc cgcataactt
     6121 ccggttgttc gggccggatg agacggcctc aaaccgttgg ggtggggtgt tcgaagtgac
     6181 cgatcgctgc tcgaccgagc agatcctccc caccgacgaa catgtggcgc acgacgggcg
     6241 tgtcatggaa atcttgagcg agcacctgtg ccagggttgg cttgaggggt acctgctgac
     6301 gggacggcat gggtttctga attgttatga agcgttcatt cacatcgtcg attccatgtt
     6361 caatcagcat gcgaagtggc tcaagacctc gcgcgagatt ccctggcggc ggccgatcgc
     6421 ctcgctcaat tatctgctga cgtcgcacgt gtggcgccag gaccacaatg gtttcagcca
     6481 tcaggatccc ggcttcatcg atcatgtggt gaataagaat gcgcaggtaa tccgtgtcta
     6541 tctgccgccc gatgcgaaca cgctcctgtc ggtcacggac cattgtttgc gcagccgcga
     6601 ttatgtgaac gtggtggtgg cgggtaaaca accggctccg caatggctgg atatggacgc
     6661 cgccgtgctg cactgcaccg ccgggatcgg gatttgggaa tgggcgagca acgaccgcgg
     6721 cagcgagccc gacgtcgtga tggcctgttg cggcgatgtg ccgaccatgg agacgctggc
     6781 ggccgtggaa atcctgcgta tcgcccagcc caatttgaag gtgcgcgtgg tcaacgtcgt
     6841 cgatctcatg aagctgcagc ctgcgagcga acatccgcac gggatgtccg acaaggaatt
     6901 cgatgcgttg ttcacgaccg acaaaccgat catcttcgcc ttccacgggt atccgtggct
     6961 gatccaccgg ctcacctacc gccggaccaa ccatgacaac ctgcatgtac gtggctacaa
     7021 agaggaaggc acgaccacca cgccgttcga catggttgtg ttgaacgatc tggaccggtt
     7081 ccacctggta tccgatgtgg tggaccgcgt gccgcagctc ggggcgcgtg ccgcctatct
     7141 caaacaggag atgcgcgaca aacgcatgtt gcacaagcag tacatcgaga cctatggaac
     7201 cgatatgccg gagatcagaa actggaagtg gagcgggaag cgctagcccg gattattcat
     7261 gaatgccgtc gcgaggcgtt cgagacggaa gcccgnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnng gaggccgaac gagaacagcc tcgccgggcg agaggatcgg
     7381 acgccggagc agggattcat gaatagtccg ggcaaggaca tggctcctcc tactgtcgtc
     7441 gtgctgttcg atgtcgacaa tacgttgctg gacaacgatc ggatcacggc cgacctcacg
     7501 cagtatttgg accgggaagt cggcccggcg cacgagcggg aatattggcg catcttcgac
     7561 gagttgcgtg aagagttggg gtatgcggac tatctcggtg cattgcagcg gtatcgcctg
     7621 gcctatccgc acgactcgca cctcctggcg gtgtcgcatt ttctggtgaa ttatccgttt
     7681 gccgaccggc tgtttccgca ggcgttggac gtcgtgcggc acgccaggca atggggccgg
     7741 acggtgatcc tcaccgacgg cgacgcggtc tttcagccgc gcaagatcga acaatcgggc
     7801 atcctgtccg tcgtcgaaga acaggtgctc gtctatgtgc ataaggaaca ggagctggag
     7861 gacgtcaggc agcgttaccc tgccgaccac tatgtgctga tcgacgacaa gttacgcatc
     7921 ctgagcgcgg tgaaagccca gtggggggcg cgtgtcacca cggtgtttcc gcgtcagggg
     7981 cactatgcca atgatcctga tgcgctgcgg acatttccgt cgcccgatct cagcatcgac
     8041 tgcatcggcg atctgttgac gatcgatcag tcgcgactct tccccgccgc aggccatcgg
     8101 tgatgccggc gcgtggcggc aggattgaca atgccagggg ctggtcgtag cctacagact
     8161 ctacctccaa cagggcaggt catcgatgac gcccaagaaa cctgacacat cgatctggtc
     8221 cacttccctc gatcgtcgcc agctgttgaa agggttcggc gtggcgggat cggtcatggc
     8281 gcttggcggg cccggtcgct tggctgagct actggccgca gacactcctt cctcgggtac
     8341 ctcgacgggc gatatccccc gccgcgcgtt gggcaaaacc ggcgtgcagg tgtcggcgct
     8401 ctgtttcggc ggagcccatt ggggccgact caaggacgaa tcagaggcct tccgcatcat
     8461 ccacgaggcc attgacgcag gcgtcaattt tctcgacaac gcttgggagt atcacggcgg
     8521 gcgatcggaa gaactcatgg gcaaggcgtt gcagggaaag cggcaacagg ttgtgctcat
     8581 gaccaaggtc tgctcgcacg gtcgcgataa aaaggtggcc ctgcagcaac ttgacgagtc
     8641 attgcgccgg ttgaagaccg actatctgga tctctggcag attcacgaag tcgtctacga
     8701 agacgatccc gatctgcact tcgcggcgaa cggggccact gaggctctgc tcgaagccaa
     8761 acagcagggg aaggtgcgat tcatcggctt caccggccac aaacatccgc agatccatct
     8821 caaaatgttg ggacacgact ttccgttcga tgcctgccag atgccgctga atgtcttcga
     8881 cagcacatat cggagttttg agcgggaggt gctgccggtt ctgaaccaac gcgggatcgc
     8941 ggctctgggc atgaagagcc tgagcgggaa tgcagagccg atcaaacaag ggatcgtgac
     9001 accggaggaa gcgatccggt atgtgttgag cctgccgatt gcgtcgctcg tcagcggtat
     9061 cgattcgccg caagtcctca agcagaacct cgacatcgcg cgccgcttta cgcctatgac
     9121 cgtcgccgag atggatggcc tgcgcaccag agtcgcgcag tacgccatgg atggccgata
     9181 cgaactcttc aaatccacca atcgctacga cggggggatc ggccgcgccc agcacgggat
     9241 ctcctagcag gctgttgata aaggccgccg gcggagttct cggtcgcgcg cctccctgcg
     9301 acgtgctcac acaccccttc gttcgcaccg cgttggtgga ccgcagatct cgaaaacgtg
     9361 cgccgtcatt cgcgcctcat cgccattcga ccgccccgaa gcctgagttg ccgagcagga
     9421 catacatcag actgacggcg ccgaccgtct ggttgcgata ttccacgctc gggtagtggg
     9481 catcccggtg agataacgtg tatcgaagcg cgacgccgtg ccggtcgaag acgcgaaggg
     9541 tgagcgcaga ttctacgcgg atgatgtttt cctgcccgtg ccgttccggc gacagaaaat
     9601 tactgacata atattcccgc cccgtcacat cgaacatcag gcgattggca tagatcaacc
     9661 ggagcgcgac cagggcctgt ggggtcgtcc cataatggta atcccgttcc tctgtgcgct
     9721 ggatgctgcc ggccgcgcca tacccggcac cgcccagcag ggtgccttgg agtgcgatgt
     9781 cttccgacag ccacgactgc cagacggttc ccaaggagag ggcggtgctg gacacccgga
     9841 acacctgcgg cgagatgtag tcgtaactgc cgaacagacc ccagattcca cgcgtcgtat
     9901 cgccggacgt gtaggctttg cccagcagca atccacggct ggtgatggtt tccagcgtat
     9961 tggccgtggc ggcagagaca tgaaaatcga aatagtcgaa cggacgggtg tacccataac
    10021 cggacttgcc tggaagtccg tatgtaaacg tgagctcacc gaccgcgcca tgttcgttga
    10081 cgctcgagga gacgttgcga ctgctcgatg tcagcgtgcc gcccgcctcc agccgcagga
    10141 atgtggccgg ccggtggctg ggatacacgc tgtcgaagcg gttgccgaat acaaggcggt
    10201 tgaatccggt gggcggcgag ataatggcgg cgcccagttc gcgccagaac cccggcgggc
    10261 catcgccggt ctcaagcaac agattggcca tcctgaacag cggttcaccc agaaacgtgc
    10321 cgccgatggg cgtcgtgatc atgtcgttga tcgaaggaga ggtccgttcg ccgccgatct
    10381 cccagaggaa actcccggcg acgctataga gcgacgattc ccagaagctg aggcctgcgg
    10441 atcgggccag accgaaatac acgctgccgc catagggatg caggaactgg ttgactgaga
    10501 actgatcgtt gtcgaacacc catttcgaat ccgtcaggtg ctcacgaatg gtcgtgccgg
    10561 tggtccggta cacgtccttt ggctcagtga aatgccgatc gtactggttc agcaggaaca
    10621 aataggtgag tacttcagcg gctggaataa ggtaactgcg ccctcgcctg ttctcccaat
    10681 ccaacgatcc ggaccggtct tttggcaggg ttgaaggttc ttgggtttca gcacgatggt
    10741 tcacgggccc gattaggcca ggctcacgcc cttgcccggc ttcatgtgcg cccgaacttt
    10801 gtgccaggcc cgcactcgac agaagcgaca gccaggcaca gacaaggaag ggcagattga
    10861 accacaggct gtgcgaacgc caacgaagca tgctgcgtca tatgccttcc ctcatcagtt
    10921 gaacgcaggg ggccgttgcc tgggtactcg gaggaaccct agtccatgct caaccgacct
    10981 gtctcgggtg ggccgtccgg ccttcgcgat cagcggcctc gcccgccgct gttgccgcga
    11041 gactgtccgc tgctgccatg tccggaaggg ccgcccagag gccggttgat cgacggggtt
    11101 tgctgcgtga tggagggcgc ggtggggatg ttcggagggc tggggcgcca gaaatgggac
    11161 gggtgcaact ggtaaggctg cggctgcggt gcagaccggt gcatcaccgg tggaatggag
    11221 cgcggtgtgg gaatggttac gatgtctccc actttggccg gtaggccctg ctggttgagc
    11281 aacggtggcg cgtgaggact ctgcatcgat ggtgaaggca cgagcaacga gcggcgcagc
    11341 gtgggattga aggcgtgagc cggggccggt acgagcgcgc cgttttgcgc cgcaatggtc
    11401 tgttgtgcct gcgcgaggtg gcgatgtcgg ggagagaggc gctgtgcctg gagtagtaag
    11461 ccgttgatcc caggtcctgc gatccaggga taggaataga agccgagcgt tgggtatgcc
    11521 cagctggtcc aggcctgtga gacaagggca ttggcttgca ggtcgttcag catgtcgtca
    11581 cgttggcggc tgagttgctg gacttcctcc gccgatgggg cggcggcgag ggcgcggttg
    11641 gtggcatcga gctgttcctg tagtcggtcg gtttcggcac ggaggaccgt gagctcccgc
    11701 atggcgctgt cgttttcccg caaggccgca atggcctgtg cgacttcatg cgggtcgatc
    11761 tctgccgtca actcagcgcg gatcacgata ctctcgttct cgaccctggt cgtgatggtt
    11821 tcctccaaca ccgtgacgat gcctgccgtg taactccgga tgtcatcacg tgtgaggttc
    11881 atgtcgcgca cttccgtgac acgttccaga tacgtggcca cctgttcgag cgcgtctctc
    11941 ttggcggctt cgaccgccag acgcgcgcca tccgtgcggg tgtcgtgatc gcccaagcga
    12001 tattcgccgc tggctgtgac gatgcgggaa tcggcggagc gcgtcggtga ttcggcaacg
    12061 gcgcgaggcg gagcagagga agcggccgga ggcccgtcga gctcctgtga tgtcagcctg
    12121 tttcgatatt gttcggggac ggactcgagt tggttcgtaa aggtttgaac cccggcctcg
    12181 tcggtgtagg tataaatcga ggcccggctg tctggtgcgg taatggcgtg ggtggagacc
    12241 ccgagaagaa agagaaccga cagtgtcagc ccactgtggg agagagacat ggtgatcccc
    12301 cgtctggtta cgaattcagg ataacacagc atcagatcgg gattccgcaa ggaggggctt
    12361 ggatatgttg ggggcaggag gggagggttt tgtcgtctcg ccttcgtttc caacttctac
    12421 atcgttctca gagcccccgc tccatctccc tatttcctgg cggacattcc aaaacgcgat
    12481 atgctggcag cagcctatgt tgctcgtcag acaaatcgct tccattctgt ttggattcgt
    12541 tcccctcgtg ctcatcgcgg tgggggtggc atcggtccta tgggaagtgc aggctcacga
    12601 tccgtcgatg ttctcggttc agggtgagaa gcaccttgcc cacgcattca tgttcgctgg
    12661 tgcgggctgc atcggactgt tcggctgcca tcgactgtgg cgggctaatg ggagctggag
    12721 gtgggttctc gtgccagcgg cggtgatgat ctttgctgtg gtctttccaa atatggaacg
    12781 ctggccacat ggaacaccgg tggagcgggc gtcgttcagc acgctgcaaa ggttgagcgc
    12841 gttcgccggt tcagcgactc aactcgcccg ggaacaggga gaatttgtct gtgacagatc
    12901 cgcaacgctc tccgcctcgt ctctgtttgc tcgacaaggt caggcgcttt cctatgtgat
    12961 ccactgtgtg cccgatgcga ccgggccgac actcggtacg cctcccgaac gacccggcac
    13021 cctcgtcttt gccgtcagcc cggatcggac acaggcctgg ttcacggcca cggtgttgac
    13081 tcacagcacc ggccggcagg catcctggtt aacgcgacaa ggccagccct tcgtcattgc
    13141 cgcacagcta caatccaaat cctaggccga catatgtgga tggtttctct tcgtgaacca
    13201 aggtcttgct gctcctctct tcgcgccggt gaaggctgtc gcaggaatac tggtgaaagc
    13261 gatttgagaa ctgccgcgga ggccggggtt cccgcatcgc ttagaatcga aggcctactg
    13321 agaccagtgc gaagttgtac caaccgaagg catagcgctc gttatctact ccgccttcga
    13381 acacacggta ccctgcggac atgaaccagt ggtcggtgat gtcataccga attttcactg
    13441 ccgcgtcgat ggcacgacct tgagtgctga cgagaccatc gaagtccagg agcgccgtcc
    13501 agcgaggggc gatgtcgtat tccaggctgc tgctcagcag gggcacgaat cccacattgc
    13561 tgtcggaagc gagcacgccc tgctggcgga gttccactcg cgcatcgcga ataaaggcgg
    13621 tggcgccgat acgccaacgc cagcgttccg tttcgtggac caggtatcga taggtgaggc
    13681 ggtaggagtc gaacttgtac tcggaatcta tcgggctgcc gggggcaaac gtgctgcctg
    13741 cgaagcgcac ttgctgtccg aatgcgccgg tccccgaaaa tccgagcggg gcgtacacga
    13801 agcgaagtga gtgccgatgg ttcatgttcc aggtcagctc gacgcggcgc tgggcttccg
    13861 gaccatggcc ttggatatcg gtcagcgcga acctcgtccc tgcgctgctg tcgggaatct
    13921 gaatctgatt acgcgattgc cagaccgccc cggtttcgaa ctccagggag aagggcaact
    13981 caggaggccc ggcgtctgcg acggaggaca gagcgctgaa catcgtggct atgacacaga
    14041 atatcaggag gatccatgcc cggtcggcag agcgtctgtt caagttgtgc gggaagtcat
    14101 tcgcgaggac gttcgactgc ttcccgtgag tgtgtgatgt cattcagaaa ctcctccctt
    14161 aatattccat aaatctccac atccacaaat cggccgaagc ggatgtaatg ctcgcgcatg
    14221 cggccttcgt aggtcatccc gacttttcgt aagacacgtc ccgaggccgg gttgttgtga
    14281 aagtgcgggg cgtagatgcg atggagtgac aggcgggtga agccgtagtc gagcaccgcc
    14341 cgaaccgctt cggtgcagta gccctggttc cagtagttgg tgccaagcca gtaactcagg
    14401 cgcgcaagtt ggtgctcgct ggcgatgtcc atcccgattg aaccgatgag tcgttcctct
    14461 tcagcgagga cgatggcaaa tgtgatggcg ttgcccgcgg cgaaactctc ctgttgctga
    14521 gcgatccatt ttgcggccgc ttcgaggtca tagggatggg ggagcaacgt gcctgcggcg
    14581 acttccttca cccctgccag gcggtgcacg gtatcggcat cggaggcctg gaattgtcgg
    14641 agaatcagcc gttcagtatg gagtgtcgga caggcatcca tcacgctgat cacagcatag
    14701 tatccagcct aaaacaattg ccgagttcgg cgtcaacctt ggtgcacgtc ataaaagcct
    14761 gtcgattgac agcgcctctg cacccggcca gaatcctgat tgtcggaaca ggcaaaagaa
    14821 atgcttcggt cttgcttccg gaatatcgac aggtcgaaac ggagggaggc aggtggtcat
    14881 gacggcgttt aatccactcg ctgcgctgcg ctctccgcgg gagacgttgg atgggtacat
    14941 catcctcccg cggttaatcg acaaagtgcg cgtgcaggca cgaggcgagc ttccggcggc
    15001 gtaccagagg aatctgctgc atcgcggggg cacgctcgac gggcgcttcc ttgcttttac
    15061 cgggctggaa ggggaggcct tgcgggcggt cattctctcc tgcgagacgg atgagccggt
    15121 ggcgcaatgg gttgcccaac atgcggcacg acattccgcc gacgagaaac agcggtgggc
    15181 cgccgaggtt gagggttatc ggccgactgg ccgtgtcctt ctttacctgc aatcgaccgt
    15241 gcctgaactg gccgcccaaa tcgattgtgc cctcgtgagc ctgctcgatc tcatcgatat
    15301 ggatgaaggc cggcttgctg tccccgcacg gggtggctct gcagatgttg aggaaagaag
    15361 cgcctgagtg gtacgcccct ctgggcgatg atttcggtcc cgaacagatg agaggggaaa
    15421 ttgcggtggc tcaacgatac aaggaacttt caaagactca tatcgaattc atcaccacac
    15481 agaagttgtt ctttgtcgga acggccacgg cggagagccg ggtaaatgtg tcgccgaaag
    15541 gcatggactc gtttcgggtg ctcggccccg ctcgcgtggc atggctcaac gtcaccggga
    15601 gcggtaacga atcctcggcc catgtacagc atgacccgcg tatgacgatc atgttctgcg
    15661 ccttcgaggg ccagccgctg gtcctgcgcc tctatggcag ggcgaaggtg gtgcacaggg
    15721 gcgactccga atggcaggag ttttttggtc tgttcccgcc gttgcccggt gcccgtcaga
    15781 ttttcgacgt gactctcgac ctcgtgcaaa cgtcctgcgg catggcagtg ccctatttgt
    15841 cgtacctcgg cgaccgggag tcgctgagcg cctgggctga gaagaaaggc gacgaagggt
    15901 tgaggcggta ccgggaagaa aagaatcagc tgagtctgga cggtattccc actcacattg
    15961 tcgacaaaga agggcactga tcccttctcg atcagcatca tacgcatccg cccggaacgc
    16021 agtcccgcac acttttcctc cgtttcttga cgcatttccg attcacgagt agggtttcgt
    16081 tggccttcta cgtaagtgga aacaatggac gtggcgcagg tcttgatcgc gatcgtcggg
    16141 ctgggagcgg ctgtgacgac gtattgcctg atgcgatttc cccacatgcg ccgtgagcgg
    16201 ttcaggcact acggggcgtt ttctattgcg gtgtcggttc tcggtgcact ggcggtcttg
    16261 ctgttctgcc tggccctgct catcctgcag aatgcctggc gagtcgggtg acgcgaggga
    16321 caattcatgc ctctcattct cggaacgatc gcaaatcggc ctgttccaaa gtgaagcatt
    16381 gtcgcagcgg cgcgacagtt tcgtgcgcaa gatcatgaaa atcccaccat gcgagcaact
    16441 cttcgccggc cgaggttgat ctggccgggt cggtctgcta cgaatactag agtgcggcat
    16501 ctggcaggaa gctcatcgtt tttccccaac tgagcgtcct ttcctcacgt cgagcccatc
    16561 agcgggttcg agtcgttcca ttacaatcga ccgttccccg cgcggctgtg tgagacgcgt
    16621 ggcggactat tttttgcggg acgttgaaac cattatgggc cttgtcagaa ccggtcattc
    16681 ccttcagctg ctgccacggc gtgaagctga ggcacaggcg gattgccttg agttccccag
    16741 gttgtccttc aagccgcccc ctcacccggt ctccatagac cgggtcgatt gggtctttgc
    16801 cgggttgctc gcactgtccg tggcgatcct gtgcctgctg attgcgcgat ccatgccctc
    16861 gttcctgttt cagtcgatgg atttctggtt cgaggccgac acgctgcgcg aaatctcgaa
    16921 catgagccgg gtgcacgatg accactatcg gacgtctgtg catccgctgt tttcgttgct
    16981 caccttcatc ccggtctata ttgcaaagca tgcgttgacc actagcccgc ttcgcgcggt
    17041 gttgctcatc accggcgtgg tgggcggctt gtgggccggg acgttgtacc tgctgttccg
    17101 cttgcttggt tgtcgcagac tggatgcctc ggtcttcacg ctgctcggcc tttgtagcgc
    17161 ctcggccctg ttctggcttc cggtgcccaa ctcctattcc tggggatcgc tctcggtcat
    17221 gctggccctc gtgctgctct tgttcgccga gcagcggccg ttctgggcta cggcctatgt
    17281 gatcgcgagt gcactgacgc tcagctttac cgtaacgaac tggatggcgg gtctgctggt
    17341 gacgctggtt cgatggccct ggaagcaagc ggtgcaactc tccgtcaatg cgttgtgtgt
    17401 ggtggtcttg ctctggggcg tgcagaagtt catctttccc acggccgaat tttttatcgg
    17461 ccatcgcgaa gaggcggagt tcatcaatca tccccaatcc gggggtgccg ccaacgtcct
    17521 gtcgtccctg gtcttccaca cggtgcttgc cccggatgtc cggttcatga aagacgatgc
    17581 gtacacccag gccaagtccg attcgtttcg cctgtccgaa cgtctgacct ttcaattttc
    17641 ccgcccggga tcgactggtc cactggggct gctggccgtc ggactctggt cggcgttgct
    17701 gttgaatgga ttgtggcggc tggtcaccct cgatcaccat cttcggttcc gcctcgtcct
    17761 ggccagcctg ctggggtttg agactctgct gcacctcctc tatggagaag aaaccttcgt
    17821 ctacagtctg aattttcttc cgcttctcct cgccgttgcc gccttggggg ccatcagtcc
    17881 cggccgtcgg gtcgtcgtgt ccctggccgc gttgctcgcg ctctgtgccg ggctgaacaa
    17941 ctggcatcag tttcaggagg ccacgcgttc ggcgacgcag ttcacgccgc agcgtgagct
    18001 catgacgacg atgatgcagc aggatccgga tcgaccctgg ccccgctcgg tggggcatgt
    18061 gccgctggcg ctgcctggaa ctcccgaggg cggcacggcc tatcacgagc cgggcgggga
    18121 tttcagtccg catgttccga gcttcggcgt ctcaatctgg ctctgcgatg ccaacgggct
    18181 tccggtcgtc accagccaga cggtaccgct cagcgatatc cagcagcggt tcgcaccctc
    18241 tacgcatccg aacattccgg ccattgtcac gaagacaccc tactatcagg cgacctggtc
    18301 gcgtctggat gccacgcatt gggaactgcg gttcaaaaat tacacgaacc atgctcccgc
    18361 aattctcatt cgcagcgtgg gcccggccgg cgggcccgtg accgcgttgg actgggacgg
    18421 ggagcagatc gggatcaacc atcgttggac ggtgaaggcg acgcctgctc ccggcgcgat
    18481 taccttgggg gatgagaata cgtccgattg gatgacagcg gagtcatcgg ccaagtcctg
    18541 gagcggcgcg tccggctggg ggtacgcgcg catgacgttt gcgccggcct caacgaggaa
    18601 ggacgatgaa gttcgtgtgg tgctttccga tttgcagccg cccgtcgaaa tccagactca
    18661 ctacacacat ccctcgaagc gggctcgtct cgcgctaccc gagccacgcc tgcaggcctc
    18721 catggatgcg caaatcacgc atctcatgat gagtctcgtc gacgatgaaa cccgccccgg
    18781 cgatcccacg ttttactacc gtgcctggca tcgacagggt tcatacatta cggcagcgct
    18841 ggcgcgggcc ggcgatcctc atgtcagccg ggtcctttca caatttctgg cctcgcatga
    18901 ttttgccggt ggctccggcc ctgaggccga tgcccccggc ctggccatct gggcgttgac
    18961 cgagtcggct gcgtatatcg ccgacccggt gcacgatcag tggctctggc cgcacatcct
    19021 ccggaaagcc caacggatcg aaggcatgtt gacggcccgc gcgccgcttg tggagccgta
    19081 ttggattcct tcgccccatg acttcaatca tgggcggcag accaggacgg cgctgttggc
    19141 ccaaccggcg cgtgacggcc tgatcgtcgg ccgcgtcgga gaggactggc cgttgttcta
    19201 tgtgaatgcc gtcagttatc gcgggttgct cgctgccgct gacttcgccg agcgactggg
    19261 gaaacgtcaa caggcggctc gttggcgaca gcatgcccgc gggttagagg cgagttggca
    19321 gcgccagttt cccaccgggt cgccggacag cgtcacgtac cccgtcagtt tgcgtgcatt
    19381 ggccgggtct gccacccgcc gcacccagtt gggacaaccg atgacgacgt atcgtccccc
    19441 tgcggtgtcc ccgacggcgg caacccaact gactcaggca catcgggcat tgcggcttgg
    19501 ccgaccggaa gcagtctggg cgacgcttca tcagctctgg agtcaccagg cctcgccggg
    19561 cctgtatacc tgggacgcgc cgcggccgac gcaggatgaa gtggcggatg gctggcaata
    19621 tgcgcggggc tggcacaatg aaaccgccgt ctcgccggac tatgaaacgg cggcgttgct
    19681 gctcttattg caacaggaca tgctggccta tctcgatgag gcggcggcgg agcccacggt
    19741 ggtgctgggc gccggtatta ctccggcctg gctttcacaa ccgatggcgg tatccaacct
    19801 cgcccttccc ggcggctcca tcacgtggca atgggacggg cagaagatgc gggtgacgct
    19861 acgcgggcct gtgcgaaaaa ttcagttggg ttcggcgttt ccgcccggca ccgagctcgc
    19921 cgtcgtgcag aagccgctgc gcccttgagt cggccgtcgg ccattcactt gagtcttctg
    19981 cgttcagaat ccccgtccac caccgccgcc gaatccgccg cctgacgatc cgccccctcc
    20041 gaacccagac ccgccggaac tacgtggggc cgacgtcatg gtgggaccgg tgcgcgcggc
    20101 catatcgcct atgcggctgg tgaagccgcg ggtgttgaac tgcgccagct cggctccctg
    20161 gtaccaggcc ggcgcggtcg tcacgatgga ttcaaaggcc cctgcccaat ttttctctac
    20221 cccgagcgcc atcgcgtagg gaagaaattt ttcgaacagt tccggagttt tgatgattcg
    20281 atcgaaacgg tcgacttcca cgcgggtcag aaattcttcg aatcccaaga cgccctcann
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagcgccgg
    20401 ccccgaggaa tgtcagcggc gccatgcccc atcggtcggc gagtgcggag agggcgaagg
    20461 tcaagagcat ccccaacacg atcccgccga tcagataccc ctgcttcacc cgatcgggcc
    20521 gctgcgtata gtacttccgc ttttggagcg actcaaagat cgcgtcctga atggggggga
    20581 gggatctgta gaagcgattt tcgagcgagc tcagcgagac gtcatcggtg gtgctgtctt
    20641 tgaacagggc ttccagcagg agccgctcgt a
//
LOCUS       DAORRQ010000069        20644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_157_length_20644_cov_46.039293, whole genome shotgun sequence.
ACCESSION   DAORRQ010000069 DAORRQ010000000
VERSION     DAORRQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20644
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157_length_20644_cov_46.039293"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..627
                     /locus_tag="PK224_16925"
     CDS             139..627
                     /locus_tag="PK224_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17482.1"
                     /translation="MRSMIHDGSQILNRSIAKLLFGLTAFLVLQTGLAAAQEAVTVQE
                     ILADPALFHLRQITLRGTARNVQPLDPYEIPAGSTCYGGYLFNLEDDTATLPVAVPGL
                     CGVPMVKDPDVEDGARVVVDATVQAPSHGGYALSFKGGQIAMDQEGVIQAIANRITPL
                     AE"
     gene            631..1581
                     /locus_tag="PK224_16930"
     CDS             631..1581
                     /locus_tag="PK224_16930"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB17483.1"
                     /translation="MALPLPEQSPRLVTPAETHVGWIGTGVMGASMCQHLQTNQYRLT
                     LYTRNRSKASLILAKGATWADSPRAVAEQTEVVMTMVGFPRDVREVYFGEQGVLAGAR
                     PGMVLIDMTTTEPSLAQEIALAAHSRGAFAVDAPVSGGDVGARNATLSIMVGGITKAV
                     QAIMPLLECLGKKIVHQGDAGMGQHTKLCNQIVIAGTMIGVCESLLYGYKAGLQLDRM
                     LESIRGGAAACWTLDNLAPRILARNFDPGFFVEHFIKDMGIALEEAQRRQLTLPGLTL
                     AHQLYEKVQALGHGRSGTHALMLALEDLSHINPVRPSAST"
     gene            1595..2326
                     /locus_tag="PK224_16935"
     CDS             1595..2326
                     /locus_tag="PK224_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17484.1"
                     /translation="MRTVPLRILLLLLLSVTVSACTGARGLNRQLLQESFHDHPEVVT
                     DRDIASTMTLQANLATPYRLAVFFKQEDFPSRPALQRVDWVSTDADRVQRALAPVQEE
                     GILQQSFLLANSTVQGTTLRDIRLAAARYNADALLIIDGASAVERYNNGHAAWYATGI
                     GAYFAHGTESHALFMMEGTLWDVRTGYLYGTQTAEGETTLIGPAPSLEDRTAVAEAKD
                     VALERFSKELAEMLRLLREKQRAMK"
     gene            complement(2545..3771)
                     /locus_tag="PK224_16940"
     CDS             complement(2545..3771)
                     /locus_tag="PK224_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB17485.1"
                     /translation="MKPALLLHNDVGSAMETAPAGFLRRLWWVLLLCTLVVPAHAGEI
                     SIAPDPIPATGTDLWHYGAYLDVGYVVNPNFPENHLWRNRATAARHNEFAPNMALAYV
                     RKDATESSRWGMELGVQGGYDSERFAFLQGEREVGGADTLRHIHRANVSYLAPVGKGL
                     TITAGLFNSLIGYESLYAKDNVNYTRSWIADNTPYMMFGVNAKYPVTDKLTVAGFIVN
                     GYYHLSHPNDQPSYGGQWAYKATPQLTLTQTVYGGPDQTNTALQFWRFYANHIVEWKG
                     ENLTFAASYDIGTENIADRPGNPRAFVMGGNVVARWHVTGPWALAVRPEFYWDRNGRW
                     TGSEQFVKAVTSTIEYRIPYKWTNTTLRLEHRWDESTGAGGGFFRRGEIQPGVLSLTP
                     NQHLVLLGILWTFDSP"
     gene            complement(3886..4593)
                     /locus_tag="PK224_16945"
     CDS             complement(3886..4593)
                     /locus_tag="PK224_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17486.1"
                     /translation="MSSTRSHDVTVLLIEDEAEIRRFLRSTLPAHGYRLYEATTGADG
                     LAQASARNPDIILLDLGLPDMNGTAVIEKIRAWSNMPIIVLSARDQEQVKVQAFDLGA
                     DDYVTKPFGVDELLARMRAALRHATRPVGDGVDASCTFGDVTVDLGRRQVFVSGAEIH
                     LTPIEYKLLTTLVRYAGKVVTHRQLLKEVWGPLHVDEGHYLRVYMRQLRNKIEADPAQ
                     PRHLVTELGVGYRLRTE"
     gene            complement(4607..7315)
                     /locus_tag="PK224_16950"
     CDS             complement(4607..7315)
                     /locus_tag="PK224_16950"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HRB17487.1"
                     /translation="MENQRPDPDALLRRVQAEEAEQVRGKLKVFFGATAGVGKTYAML
                     QAAHEQQRDGIDVMIGWVETHGRAETEALVQGLPVLPSREVVYGGSRLRDFDLDAALS
                     RRPQLLLMDELAHTNAPGSRHPKRWQDVRELLQAGIHVYTTVNVQHIEGLNDAVAQIT
                     GIRVSETVPDSILEQADDVELVDLPPDDLLQRLKDGKVYLPDQAQHAIKHFFRKGNLI
                     ALREMALRRTAERVDQQMEVYRRDHAVVGTWPAAETILVCVNLKSRGPMLVRAARKMA
                     TGLHARWIAVYVETSIHLRLTEQERSQGVDTLRLAERMGAETVTLTGDDVSAQLLAYA
                     RSRNVTKIIVGKPLRSRWKEWLFGSVVDDLVRGSGDIDIYVMTGEAGTGQPLVTGLLQ
                     RTSERRNYGYASALVMACTALSGVMAPYFGQANLIMVYLFGVVVIATRWGRGPSALAS
                     LMSVAAFDFFFIPPYYSFAVSDVQYLLTFAVMLVVALLTSGLASHKSRQAESALIREQ
                     RTAALYAMSRELITQREVNKLASVASRHIHDVFHCQVAVFLPDRDGRVHLHRGDALHF
                     DLDPKEAGISQWVFDHKESAGHGTNTLSGSDSLYLPLLGSHGAVGVLAVRSSRPAELL
                     APEQLHLLETFANQMALALERARLAEETQEAHVQTETERMRNAVLSSVSHDLRTPLAT
                     IVGASSAILNTTRPLSIESQHELVRSISDEAGRLDRLLKNLLDMTKLEAGAMQLRKEW
                     HALEEVVGTALARVKSRIATHPVSTTFPDSLPLVLVDGVLIEQVLINLLENAAKYSPP
                     ASPIDVTASVKEGMIVTEVADRGPGIPRGEERRIFEKFYQLHPDREGGVGLGLTICRG
                     IIESHGGRIWVEARSGGGSSFCFTLPTEHNQPTVEPEQNGHDRAVT"
     gene            complement(7401..7982)
                     /gene="kdpC"
                     /locus_tag="PK224_16955"
     CDS             complement(7401..7982)
                     /gene="kdpC"
                     /locus_tag="PK224_16955"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HRB17488.1"
                     /translation="MVVQLRSALMMFFILTILTGVAYPLAVTAIAQLLFPHQANGSLI
                     SKDGKPIGSTLIGQPFDDPKYFWGRPSATAPFPYNAAASSGSNLGPTNPALIETVKMR
                     VAALKAADPGNDTPVPVDLVTASGSGLDPHISPASAEYQVRRVARARGREEAFVRTLV
                     SQHTEGRQLGMLGERRVNVLALNLALDATDPPR"
     gene            complement(7993..10104)
                     /gene="kdpB"
                     /locus_tag="PK224_16960"
     CDS             complement(7993..10104)
                     /gene="kdpB"
                     /locus_tag="PK224_16960"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRB17489.1"
                     /translation="MTTSTKARPLFDPPLVRQALLGALRKLDPRIQIRNPVMFVVFVG
                     SLFTSLLFLQALFGTGEAPTWFILAISLWLWFTVLFANFSEAIAEGRGKAQADSLRRA
                     RTELTAKRFGKPTSGEDYLAVPAAIGKPGEVYCLVPASFLKKGDIVLVEAGDDIPSDG
                     DVIEGIASVNESAITGESAPVIRESGDRSAVTGGTRVLSDWLVVRITASAGETFLDRM
                     IAMVEGARRQKTPNEIALTILLSALTVVFLLATVTVLPFSLYSAHAMGEGVPITVTVL
                     VALLVCLIPTTIGALLSAIGIAGMDRMIQANVIAMSGKAVEAAGDVDVLLLDKTGTIT
                     LGNRQATAFISAEGVQERSVADAAQLASLADETPEGRSIVILAKERYGLRGRDIHAMG
                     ATFLPFTAHTRMSGVDFDGRQIRKGAAEAIEDYVTQLGGAFPPAVRNAVETIATQGGT
                     PLVVAEGKSVLGVIHLKDVVKGGIKERFAELRRMGIKTMMITGDNQKTAAAIAAEAGV
                     DDFLAQATPEAKLKLIRDLQAGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEA
                     GNLVDLDSNPTKLIEIVEIGKQLLMTRGALTTFSIANDIAKYFAIIPAAFAGTYPALG
                     ALNVMGLATPESAVLSAVIFNALVIIALIPLALRGVKFRPIEASLLLRRNVLVYGLGG
                     IVAPFIGIKLIDLVLVALGLV"
     gene            complement(10271..12076)
                     /gene="kdpA"
                     /locus_tag="PK224_16965"
     CDS             complement(10271..12076)
                     /gene="kdpA"
                     /locus_tag="PK224_16965"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRB17490.1"
                     /translation="MTVNGLAQIGLFFFVLIALVKPLGWYMARVYTGQACGMDRVMGP
                     FERLIYRVCGVRATEEMDWKTYAVAMLLFNVTGLVALYVLQRLQGYLPLNPTGFGAVA
                     PDLAFNTASSFITNTNWQAYAGESMLSYLTQMLGLTVQNFVSAATGMAILVALIRGLT
                     AQTAATIGNVWVDLTRSTLYILLPLSAVLALVLVSQGTVQTFGSSHHTTLLQSVTYEK
                     PIVDAMGQPVLDEKGAAKTESTAGTEQALAVGPVASQVAIKHLGTNGGGFFNANAAHP
                     YENPTPLTDFMLILAETVIAAALTYTFGTMVGDTRQGWAILAAMLSVLALFVLGAYWA
                     ESAGNPRLTMLGVEQTAGSAQSGGNMEGKEVRFGVARSALFATATTATSTGAVNAMHD
                     SFTPLGGLVPLFMMQFGEVIVGGVGSGLYGMLIFAIIAVFIAGLMVGRTPEYLGKKIE
                     TYEMKMASLLILIMPIIVLGFTAVAVVTESGTSSILNPGAHGFSEILYAYTSQGNNNG
                     SAFGGLNANTPFYNLTGALAMLVSRFWLAIPTLALAGSLARKTIVPAGPGTLPTHRPL
                     FVGLLVGVVIMVGALTFVPALALGPVVEHLLMVAR"
     gene            complement(12076..12165)
                     /gene="kdpF"
                     /locus_tag="PK224_16970"
     CDS             complement(12076..12165)
                     /gene="kdpF"
                     /locus_tag="PK224_16970"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HRB17491.1"
                     /translation="MNAMYALGAVLSLGLLVYLMIALLKAEWF"
     gene            complement(12825..13346)
                     /gene="nuoB"
                     /locus_tag="PK224_16975"
     CDS             complement(12825..13346)
                     /gene="nuoB"
                     /locus_tag="PK224_16975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRB17492.1"
                     /translation="MFRILKKSLQTGVVTGHHPAGAAPSEPVSQDAIEKAKPFRRSLT
                     IREVDTGSCNACEMEMNALANPVYDVERFGVHIAASPRHADALVVTGPVTVNMERALK
                     DVYKATADPKIVIALGDCAVNCGLFKGSYAVTGPVDRHIPVDVRISGCPPRPAEILAV
                     LGALRKNKADSDQ"
     gene            complement(13399..14991)
                     /locus_tag="PK224_16980"
     CDS             complement(13399..14991)
                     /locus_tag="PK224_16980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRB17493.1"
                     /translation="MIDARPAVEQMQAAFPQALTEVCAVHGIPMLRLKKQDLPTVAHF
                     LHTNPNLRGSLSLLWAVDHRPREARYELCYLFTLAERKDWLLLATDLHGDEREFPSIT
                     PHLHAAKWYEREIRDLFGLIPVGHPDLRRLVRHEHWPKGSHPLKKDFPWDRVLGRVQG
                     EYAFRKIHGEGVFEVPVGPIHAGIIEPGHFRFSVAGEPIMQLEVRHFWKHRGVEKLFE
                     QQRLTEAVPLSERVSGDTSVGHSLAYCQAVETLQGVEVSPRARYLRTLFLELERLHNH
                     IGDVGAICNDTAYALAHAHCGRMKEQLMQLNDRLTGSRFLRGVNCVGGVALDLSGLQL
                     AQVEVELNTLEQDFSAIEKIVFANASLTERLENTGILTEGIAWDHAVIGVVGRASGID
                     RDLRRDRPFAAYDVLQPKVALYRYGDVRARMRVRLDEIHESIRLIREVRRGLPLGRLV
                     TRPMHEAQPGVWALSAVEGWRGEILYVVMAGDADRIHRCKVRDPSFVNWPAIQWAALG
                     NIVPDFPLINKSFNLSYAGNDL"
     gene            complement(14996..16528)
                     /locus_tag="PK224_16985"
     CDS             complement(14996..16528)
                     /locus_tag="PK224_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HRB17494.1"
                     /translation="MWSVIVLLTGPVLAGLLSLVIHRARVLHVVNFSTMLALAVAETA
                     LTRQVLAEGSVTTLGGFVYVDALSDFILMIITAIGLSCSLYMWSYMDDRVARGIIAPK
                     RLGHFFFLFHMFLFAMVAATVANSLGVQWVALEGTTLATTFLIAFFRRRESLEAGWKY
                     LILCSVGIALALFGVVLTYYSSVRVLGDASSALNMTALIGVANQLDPNVLKLAFIFML
                     VGYGTKVGLVPMHTWLPDAYSEAPAPIAAMLAGVLETVAVYTVLRSKALVDQALPPEF
                     TGNLLLTFGLLSFIVASLFILIQHNYKRLFAYSSIEHMGLAMIGFGVGGMIGTFGGLF
                     HLLNHAVAKALAFFVAGNIHRRFDTLEIDGVRGLARAQPITAVAILVAGCALVGLPPF
                     SPFISELLVVSAVAAQDFSSDTMHVGRFVTMTISDEMRSLGIVALFLFFAVVLFGGFM
                     FRVGAMVWGAPPAGIAQGESWTAGHVPLMIMIVALLGLGFVLPDPIQTLLTRAVNVIV
                     VR"
     gene            complement(16566..17231)
                     /locus_tag="PK224_16990"
     CDS             complement(16566..17231)
                     /locus_tag="PK224_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HRB17495.1"
                     /translation="MPASTQLGSQLVDLCSVLLLLCCFAIVAQRRLSACVDLFALQSA
                     FLAATAALVAFLTGHKHIYFAAALTIVIKVIVLPRILRKVIERLNVSRELGMNINIPA
                     GLLICGALVIIAFFITQPIIPLGYLLTRDSLAIALAIILIGFFTMIARQKAITQMVGF
                     LVMENGVFLGATAAAYGMPLIVELGVFFDVLVAGLIVGIYTHRLQDTFDSVDTSTLTV
                     LKE"
     gene            complement(17236..18192)
                     /locus_tag="PK224_16995"
     CDS             complement(17236..18192)
                     /locus_tag="PK224_16995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRB17496.1"
                     /translation="MQSIIHIVLVVVQLVLLLTLSPFVVGLIRKVKARLQCRRGASLF
                     QPYADLAKLFRKQPVISSTTSWIFTAAPYIVFASTVAAGLLMPVFVSHTPLNFAGNII
                     ALVYLLALGTFFLILAGLDAGSAFGGMGSSREAIVATLAEPAMMLSILSIALTAGSTN
                     LSTIVHQSALMEGVVVAPPAHLMALAALFIVTLAETGRVPVDNPATHLELTMIHEAML
                     LEYSGRYLALMEWAAGIKLLVFLTLIVNVFAPWGIATSLAPSAIAVGLLVYLGKVAAL
                     ALVIGVIESMFAKLRLFRVPEMLGAAFILSLLALVFFYTLKG"
     gene            complement(18257..20281)
                     /gene="hyfB"
                     /locus_tag="PK224_17000"
     CDS             complement(18257..20281)
                     /gene="hyfB"
                     /locus_tag="PK224_17000"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HRB17497.1"
                     /translation="MTALLSVLLVWYLLGGLLPLCTTRPHLQNLFAHGCAAAAGVVGI
                     VLGVAGLLSTAPLTLSLASNMPHLTFAIRLDPLAAFFVLTISLVGVAASIYASGYVEE
                     FSGRVSVGLLGSLLNGFLLSMTLVVLADNGFFFLILWEVMSLLSYVLVVTEHEKPGVR
                     EAGLFYLIMTHVGTAFIILTLLIFSQETGSFSFEAFRHPDQQLPEGLRSIAFFTALIG
                     FGAKAGIVPLHVWLPYAHPAAPSHISALMSGVMIKTAIYGLIRVYFDFMGGVFPWWWG
                     FTLLVAGTVSALLGVMYALMEHDLKGLLAFHSVENIGIILLGIGAGMIFHTYGLHELA
                     ALGLLAGLYHTINHAAFKALLFLGAGALQFSTHTRHIEEYGGLLRRMPWTGAFFLIGA
                     VSIAALPPTNGFVSEWLVFQSLFLSFQLPTLFLKLMLPIAAAMLALTGALALACFAKA
                     FGMSFLAQPRSAHARHATEVPWSMRVGMGFLAGLCIVLGLAPMLVVPLLDRVTAPLTG
                     AAISSQVLALDGWVVAPVTVEFSSISTPVLAMLLVGAGALGLLIARLCGKGLRARYYK
                     TWGCGLNLTPRMEYTATGFAQPIKQVFETIYQPTVKLEQEFLEQSKYFIKHQRFESHI
                     EPVFEKYLYRPVVTLLLATADRLRIIQAGSLHLYLTYIFVTLVLLLLWAG"
BASE COUNT         4668 a         6393 c         5841 g         3742 t
ORIGIN      
        1 gggcgaatgt ccttaatgta ggtatctaac tggcgctgca agtcgctcat cgctgtgcct
       61 ccctgtgatc gccggcgatt atatcgactc gcaggcggaa agcctatgag ggcgatgatc
      121 gagacaaaga atggtagaat gcgctcgatg atccacgatg gctcccagat cctgaatcgc
      181 tctattgcga aactgctctt cggcctgacg gcgttcctgg tcctccaaac cggcctggcc
      241 gccgcacaag aagccgttac cgtgcaggaa atcctggccg atcccgcgct gttccacttg
      301 cggcagatca cgttgcgagg gacagcccgc aacgtccagc cgctcgatcc ctatgagatc
      361 cccgccggct cgacctgtta tggcggctat ctcttcaact tggaagatga cacggccacg
      421 ctaccggtcg cagtcccggg actctgcggg gtcccgatgg tcaaggaccc tgacgtggaa
      481 gacggggcac gcgtggtagt ggacgccacg gtccaggccc cgagccatgg agggtatgcc
      541 ttgagcttta agggcggaca gatcgccatg gatcaagagg gcgtcataca ggccatcgcg
      601 aaccggatta cgccgctggc ggaataactc atggcgcttc ctctccccga acaatctccc
      661 cgcctcgtga cccccgctga gacgcacgtc ggctggatcg gtaccggcgt gatgggcgcc
      721 tccatgtgcc agcatctcca aaccaaccag tatcgactga ccctctacac caggaaccgc
      781 agcaaggcgt cgttgattct cgccaagggc gcgacctggg ccgattctcc tcgcgcagtc
      841 gctgagcaga cagaggtcgt gatgacgatg gtgggattcc cccgtgacgt acgtgaagta
      901 tacttcggcg agcagggtgt gctggcaggc gcgcgtccag gcatggtgct gatcgatatg
      961 accaccaccg agccgtcgct cgcgcaggag attgcgctgg cggctcactc acgcggggcc
     1021 ttcgccgtgg atgcgccggt ctccggcggc gatgtcggcg cgcgcaacgc caccctctcc
     1081 atcatggtcg gagggattac caaagcggtt caggccatca tgcccctgct ggaatgcctc
     1141 ggcaagaaaa tcgtgcacca gggcgatgca ggcatgggac agcatacgaa gctctgtaac
     1201 caaatcgtca tcgccggcac catgatcggc gtctgcgaga gtctcctgta tggctacaag
     1261 gcggggctgc agctagatcg catgctggaa tcgatccgcg gcggcgcggc agcctgctgg
     1321 acactggaca atttagcgcc acgcattctg gcccgcaatt ttgacccggg gttcttcgtc
     1381 gaacatttca tcaaagacat gggcattgcg ttggaagaag cgcaacggcg acagctcacc
     1441 ttgcccggac tcacactcgc gcatcaatta tatgaaaagg tacaagcgct cggccatggc
     1501 cgatccggca cccatgccct aatgctggca ctggaagatc tgtcgcatat caatccggtc
     1561 aggccttcgg catcaacgta accacgggag ttctatgaga accgttcctc tgcgcattct
     1621 tctccttctt ctcttgtcgg tcacggtctc cgcctgcacc ggagcccgcg gtctcaaccg
     1681 tcagctcctg caggaatcgt ttcacgatca tcccgaggtg gtcaccgatc gtgatatcgc
     1741 atccaccatg accttgcagg cgaacctggc gacgccctat cgcctggctg tttttttcaa
     1801 acaggaagat tttccaagcc gcccggcgct gcaacgggtg gactgggtca gcacagacgc
     1861 cgaccgtgtg caacgggcgc tcgcgccggt acaggaagaa ggcattcttc agcagagttt
     1921 cctcctcgcc aactccacgg tccaagggac cacactccgt gacattcgcc tggccgccgc
     1981 ccgctacaac gccgatgcgc ttctgatcat cgatggggcc tccgccgtgg aacggtacaa
     2041 caacgggcac gccgcctggt acgccaccgg catcggcgcc tacttcgccc acggcaccga
     2101 aagccacgcg ctgttcatga tggaaggaac gttatgggat gtccggacag gttatctgta
     2161 cggcacgcaa acagcggaag gggagacgac gctcatcggc ccggcgccat cgcttgagga
     2221 caggaccgca gtggccgaag ccaaggatgt ggccctcgaa cggttcagca aagaactcgc
     2281 cgaaatgctc cggctcttga gggagaagca gagagcgatg aagtaagact tctaccgctc
     2341 cttattccga ttgtggcccg gatgttttct gcgcatcgtc gcgaggcagt tgaagccgaa
     2401 gcccgccgcg gaacttggtc tctcgggcgc tcaacatggc tgtcgggcga ggccgcagtg
     2461 agcgagaggc tgaggcgttc ccggcagtac gtcgaaggct tgagcgaagc gagaacgaag
     2521 cccgaagcca ttttcagtgc ccggtcacgg agagtcgaag gtccagagaa tgcccagcaa
     2581 taccagatgc tgattcggcg tcaggctcag cacaccaggc tggatctccc cacgcctgaa
     2641 gaatcccccg cccgccccgg tcgattcgtc ccatcgatgt tcaagccgca gcgtcgtgtt
     2701 ggtccacttg taggggattc gatattcgat cgttgaggtc acggccttga cgaattgctc
     2761 cgaaccggtc caacggccgt tccggtccca atagaactcc ggccggaccg ccagagccca
     2821 aggtccggtc acatgccatc gcgcgaccac attgcctccc atcacaaatg ccctgggatt
     2881 tccgggacga tcggcgatgt tttcggtccc gatatcatag gaagccgcga atgtcaggtt
     2941 ctcccctttc cattcgacga tgtgattcgc gtagaagcgc cagaattgca gtgccgtgtt
     3001 ggtctgatcc ggccccccgt agaccgtctg ggtcagggtg agttgcggag tcgctttgta
     3061 ggcccactgc cccccataac tcggttggtc gttcggatgc gacaggtgat agtatccgtt
     3121 aacgataaac cccgcgaccg tcagcttgtc ggtgaccgga tatttcgcat tgacgccgaa
     3181 catcatgtag ggcgtgttgt cggcaatcca tgaccgggtg tagttcacat tgtccttggc
     3241 atagagcgat tcgtagccga tgaggctatt aaaaagcccg gcggtgatcg tgagcccctt
     3301 cccgaccgga gccagatagg acacattcgc acgatggatg tgccgaagcg tatcggcccc
     3361 gccgacttca cgctcaccct gtaaaaacgc aaagcgttcg gagtcgtagc cgccttgcac
     3421 accgagttcc atgccccaac gcgatgattc agtcgcgtct ttccgaacat aggccagggc
     3481 catattggga gcaaactcat tgtgccgcgc cgccgtcgca cgattccgcc agaggtgatt
     3541 ctctggaaag tttggattca cgacgtagcc gacatcaagg taggccccgt aatgccagag
     3601 gtcagtgccg gttgccggaa tcggatcggg tgcgatgctg atttcaccgg catgcgccgg
     3661 tacgaccaac gtacagagga gaagtaccca ccagagtcgg cgcaaaaaac cggcaggagc
     3721 agtctccatg gctgagccga catcgttatg caacagcaaa gcaggtttca tctgacactc
     3781 atcgctccac cttctcacgg catagacatt aggtgatgtc gcatcaaaac aggattaaaa
     3841 gcgaacatgg aggcgtaaag aatctgtaaa agtccgttgg agctctcatt ctgttcggag
     3901 acgatatccg acacccaatt ccgtcactaa atggcgcggc tgagcgggat cggcttcgat
     3961 cttattcctc aattgacgca tatacacacg caaatagtgt ccctcgtcta catgaagggg
     4021 accccacact tctttgagca actgacgatg cgtcaccacc ttcccggcat accgcacgag
     4081 cgtcgtcagc aacttgtatt caatcggcgt gaggtggatc tccgcccccg agacaaacac
     4141 ttgtcttcgc cctaaatcga cggtcacatc gccgaacgta cagctcgcgt ccaccccgtc
     4201 tccaaccggg cgagtcgcat gcctaagagc cgcgcgcatg cgggccagca attcatccac
     4261 gccaaacggt ttcgtgacgt agtcatcggc accaagatcg aaggcttgca ccttgacctg
     4321 ttcctggtct cgtgcggaaa gcacgatgat cggcatgttc gaccaggcgc ggattttctc
     4381 aatcactgcg gtgccgttca tatcgggaag accgagatcg agcagaataa tgtcgggatt
     4441 gcgagcggag gcctgcgcca gtccatccgc gccggtggtc gcttcgtaca gccgatagcc
     4501 atgagccggc aaagttgagc gaaggaaccg cctgatctcc gcctcatctt cgatgagcag
     4561 cacggtcacg tcatggctcc gcgtcgatga catgtgcaaa cagatcctag gtgactgcgc
     4621 ggtcatgccc gttctgttcc ggttcgaccg tcggctgatt gtgctctgtc ggaagcgtga
     4681 agcagaagct cgatccgccg ccggagcggg cttccaccca aatgcgcccg ccatgggatt
     4741 cgataatccc tcggcaaatc gtcagtccca gcccgacgcc accttcccga tcgggatgga
     4801 gttgataaaa cttttcgaag atcctccgct cttcccctcg cggaataccg ggccctcgat
     4861 ccgcgacttc cgtcacgatc atcccttcct tcaccgaggc cgtcacatcg atgggggagg
     4921 ccggaggaga atatttcgca gcattttcca ataaattgat cagcacttgt tcgatcaaga
     4981 cgccatccac gagcaccaga gggagactgt ccgggaacgt tgtcgacacc ggatgtgtag
     5041 cgatgcgcga tttcactcga gccaacgccg taccgaccac ttcctccagc gcatgccatt
     5101 cctttcgcag ctgcatggca cccgcctcca atttggtcat atcgagaagg ttcttcagca
     5161 accgatccag acgaccagcc tcatcgctga tggaccggac cagttcgtgt tgactctcaa
     5221 tcgacagggg ccgggtcgtg ttcagtatcg cgctgctcgc tccgacaatc gtggcaagcg
     5281 gcgtcctgag atcatgggac actgagctca gcacagcgtt tcgcatgcgc tccgtttcag
     5341 tctgcacgtg ggcttcttgt gtttcttccg ccaggcgcgc acgttccaac gccaacgcca
     5401 tctgattcgc aaaggtctcg agaagatgta gctgctccgg agccagtaat tcggcgggtc
     5461 gcgacgatcg caccgccagg acaccaaccg ccccatgaga tcctaacaat ggcagataga
     5521 gcgaatccga tccggaaagg gtattcgtgc catgaccagc agattcttta tggtcgaaga
     5581 cccattgcga gatgccggct tctttcggat cgaggtcaaa gtggagcgca tcgcctcgat
     5641 ggagatgcac ccgcccgtct cgatcgggca gaaacaccgc cacctggcaa tggaacacgt
     5701 cgtggatatg acgggacgcg acagaagcca gcttgttcac ttcacgctga gtgatcaatt
     5761 ctctactcat ggcgtagagc gcggcagttc gttgttcacg aatcaaggcc gactcggcct
     5821 gccggctctt gtgagacgca agcccgctgg tcagcagcgc gaccacgagc atgaccgcaa
     5881 atgtcagcag atattgcaca tcggaaacgg cgaatgaata gtaaggggga atgaaaaaga
     5941 aatcgaaggc cgcgacgctc atcaatgacg ccagcgccga aggacctcgt ccccatctgg
     6001 tggcaatgac caccaccccg aacagataca ccataatcag attggcctgc ccgaagtatg
     6061 gagccatcac cccactcagc gccgtgcagg ccatcaccaa cgccgaggcg tagccgtagt
     6121 tccggcgctc actggtccgt tgaagtaatc cggtcacgag aggttgaccg gtccccgctt
     6181 caccggtcat gacgtagata tcgatatctc cgctcccgcg aacgaggtcg tccaccaccg
     6241 aaccgaacag ccattccttc cagcgcgagc gcaatggttt tccgacaatg attttagtga
     6301 cattgcggct ccgggcatac gccaacagct gcgcactcac gtcatccccg gtcaacgtca
     6361 ctgtctctgc gcccatacgc tcggccaacc gcagggtgtc gacaccttgg ctccgctcct
     6421 gctcagtcaa ccgcagatgt atggacgttt ccacatagac cgcgatccac ctggcatgga
     6481 ggcccgtagc catctttctg gccgcgcgaa ccaacatcgg cccgcgggat tttagattca
     6541 cgcagaccag gatggtttcc gctgccggcc aggtgccgac gaccgcatgg tcccggcgat
     6601 acacttccat ttgttgatcg actcgctcag ccgtccgacg cagagccatc tcacgcaacg
     6661 cgatcaagtt gcctttccga aagaagtgtt tgatggcatg ctgcgcctgg tccggcagat
     6721 agaccttgcc gtccttgagc cgttgcagga gatcgtccgg gggaagatcc accaattcga
     6781 cgtcgtcagc ttgttcgaga atggaatccg gcacggtttc gctcacgcga ataccggtaa
     6841 tttgggccac ggcgtcattg aggccttcga tatgctgcac gttgacggta gtatagacgt
     6901 ggataccggc ctgcaggagt tccctcacat cctgccaccg cttgggatgc ctggacccag
     6961 gggcattcgt atgcgccagt tcatccatta gcaaaagctg cggccggcgc gacagggctg
     7021 cgtccagatc gaaatcacgg agcctggagc ctccatatac cacttcccgg gatggaagaa
     7081 ccggcaaccc ctgaaccagg gcttccgttt cggcccggcc gtgagtctca acccacccga
     7141 tcatcacatc gatgccgtcg cgttgctgct catgtgccgc ttgcaacatg gcataggtct
     7201 tcccaacccc tgcagtcgcg ccgaaaaaca ccttcaactt gccgcgcacc tgttcggcct
     7261 cttcagcctg cacgcgcctc agcaaggcat cggggtccgg acgctggttc tccatggcac
     7321 tcctgacaaa ggaatctgat gatggatgcg agatgacaca agattggcag agcgaattct
     7381 aaaagacaga cgtaaaagcg tcaacgagga gggtcggtcg catccaatgc aaggttcaac
     7441 gccagcacat tgacccggcg ttcaccaagc atccccagct gacgtccttc ggtatgctga
     7501 ctcaccaacg tccggacgaa tgcttcctcc ctcccccgtg cacgagcaac ccggcgtact
     7561 tggtattcgg ccgaagcggg gctgatatgt gggtcgagac cgctacctga ggcggtcacg
     7621 agatcgaccg gaaccggtgt gtcattgccg ggatccgcgg ctttcaacgc agccaccctc
     7681 attttgaccg tctctatcag cgcgggattg gtagggccca gattcgaacc ggatgaggcc
     7741 gccgcattgt aggggaaggg agcggtcgcg gaagggcgtc cccagaaata cttgggatcg
     7801 tcgaagggtt ggccgatcag cgtagaaccg atcggcttgc cgtctttaga gatcagactc
     7861 ccattggcct gatgggggaa cagcagctgc gcgattgctg tcacggccag cggataggcc
     7921 acgccggtca ggatcgtcag aatgaaaaac atcatcagcg cggatcgaag ttgaacgacc
     7981 attgtgatct cctcagacca atcccagggc gaccaggacc aggtctatca gctttattcc
     8041 aatgaacggg gcaacgatcc ctcccagtcc gtacaccagc acattgcggc gcagcagcaa
     8101 ggaggcttcg atcggccgga acttgactcc ccgaagcgcg agcggaatca gcgcgatgat
     8161 gaccagggcg ttgaaaatca cggccgacag tacggcgctt tccggcgtcg ccagtcccat
     8221 gacattcaac gcgcccaggg ccggataggt gccggcaaac gccgccggaa tgatcgcaaa
     8281 atacttcgcg atatcgttag cgatactgaa ggtcgtgagc gcgccgcgcg tcatgagtag
     8341 ctgtttgccg atttcgacga tctcgatcaa tttggtggga ttggagtcca gatcgacgag
     8401 attgcccgct tctttggcgg cctgggtgcc ggtattcatg gccacggcca catccgcctg
     8461 cgccaacgcc ggcgcgtcat tggtgccgtc gcccgtcatg gccacgagtc ggcctcctgc
     8521 ctgaagatca cggatgagtt tcaatttcgc ttcgggagtg gcctgtgcga gaaaatcgtc
     8581 cacgccggcc tctgcggcga tcgcggcagc cgttttttgg ttgtcgcccg taatcatcat
     8641 cgttttgatg cccatgcggc gcaattcggc aaaccgttcc ttgatgcccc ccttgaccac
     8701 gtctttcaaa tggatcaccc caagtaccga cttgccttcg gccacgacca gaggagtgcc
     8761 accttgcgtg gcaatcgtct cgaccgcatt tcgcacggcc ggcggaaacg cccccccgag
     8821 ttgggtcacg tagtcttcaa tggcttccgc cgcacccttc ctgatctgtc gaccgtcaaa
     8881 gtcgacgccg ctcattcgcg tgtgcgccgt gaacggcagg aaggtggccc ccatcgcatg
     8941 gatgtcgcgc cctctgagtc catagcgctc cttcgccaga atcacgatgc tacggccctc
     9001 aggcgtttca tccgcaaggg atgccaattg ggccgcatcg gcgacagatc tttcctgcac
     9061 tccttcggct gaaataaatg cggtggcttg ccggttcccc agcgtaatcg tccccgtttt
     9121 gtcgaggagc agcacatcca catcaccggc cgcttcgacg gctttccccg acatggcgat
     9181 cacgttcgcc tggatcatgc ggtccatacc cgcgatgccg atcgcggata agagcgcgcc
     9241 gatcgtcgtc ggtatgaggc agaccagcag ggccaccagg accgtcaccg tgatgggcac
     9301 cccctctccc atggcatgcg cggagtagag agaaaacggc agcacggtca cggtcgccag
     9361 caagaagacg acggtgagag ctgacaacag aatagtgagt gcgatttcat tcggtgtctt
     9421 ctggcgcctg gcgccctcaa ccatggcgat cattcggtcg aggaaggttt caccggcgct
     9481 ggcggtgatc cgcaccacga gccaatcgga cagcacccgc gtgccgccgg tgacggcgct
     9541 ccggtcaccg gactcacgaa tcaccggcgc gctctcgcct gtaatcgcac tttcgttgac
     9601 ggatgcaatc ccctcgatca catctccatc gctgggaatg tcatcgcccg cctccaccag
     9661 tacgatgtcg ccttttttga gaaaactggc cggaacgagg caatagacct cgccgggttt
     9721 gccgatggcg gcgggcaccg ccagatagtc ttctccggaa gtcggttttc caaatcgctt
     9781 ggcagtcaat tctgtccgag ccctccggag agaatcggct tgagccttgc ctctcccttc
     9841 ggcgatcgct tcggaaaagt tggcgaagag cacggtgaac cagagccaca gcgagatggc
     9901 gaggatgaac caggtcggcg cctcccctgt cccgaacaat gcctggagga acaggagcga
     9961 cgtgaagagg ctccccacga agacgacgaa catgaccgga ttgcggatct gaatgcgagg
    10021 atcgagcttt cgcagtgcac ccagcagggc ttgtcgcaca agcggaggat cgaagagcgg
    10081 tctggccttc gtcgatgtcg tcatgtgact ccctccgctc attcaggcta gaaaacattc
    10141 ccccaggatg ctcaaaacgt tcgtccagca aggccgcagc gaatgaacat ccgaggcgta
    10201 cccttgcggt acgttgagga tctgaatgac gcgagaacgc cgctggcgga cgttttcagc
    10261 atcctgtaag ctaccgagca accatcagca aatgttccac gaccggtccc aacgccaggg
    10321 caggaacgaa ggtcagggca ccgaccatga tcaccacccc gaccagcaat cccacaaaga
    10381 gcggccggtg agtcgggagc gtacccggac cggccggcac gatcgttttg cgcgccaacg
    10441 atccggcgag tgccagtgtc gggatcgcca accagaatct ggagaccagc atcgccagag
    10501 caccggtcag gttgtaaaac ggcgtattgg cgttcaatcc cccgaaggcg ctgccgttgt
    10561 tgttaccctg agacgtatag gcatagagaa tttcgctgaa accatgcgcg ccgggattga
    10621 gaatggacga cgtgccggac tccgtcacga ctgccactgc cgtgaacccg aggacgatga
    10681 tgggcatgat gagaatcaac agagatgcca tcttcatttc atacgtttcg atctttttcc
    10741 ccagatattc cggggtccgg cctaccatca ggccggcaat gaacaccgcg atgatcgcga
    10801 agatcaacat cccgtagagg cccgagccga ccccgcccac aatcacctcg ccgaattgca
    10861 tcatgaacag cggcaccaac ccgccgagtg gcgtgaatga atcgtgcatg gcattcaccg
    10921 ctccggtgga tgtggccgtc gtcgcggtgg caaagagcgc cgaacgggcg accccgaaac
    10981 gaacttcctt cccttccatg ttgccgccgg attgcgcact cccggcggtc tgctcgacgc
    11041 ccaacatagt caggcgtgga tttcctgcgg actccgccca gtaggccccc agaacgaaga
    11101 gggccagcac gctcagcatc gcagcgagga tcgcccagcc ctgcctggtg tcgccgacca
    11161 ttgttccgaa cgtgtaggtc agagcagcgg caatcacggt ttcagccagg atcagcatga
    11221 aatcggtcag cggcgtcggg ttttcatagg gatgagcggc attggcatta aaaaatccgc
    11281 caccgtttgt tccgaggtgc ttgatggcca cttgtgacgc aaccggaccg acggccaagg
    11341 cttgctcagt ccctgctgtt gattcggtct tggcagcgcc cttctcgtcc aacaccggct
    11401 gccccatggc atcgactatc ggcttctcat aggtcacgga ctgaagaagc gtggtgtgat
    11461 gagaagaccc gaaggtctgg accgtccctt gcgagacgag aaccagcgcg agaacggctg
    11521 acagcggtag taagatgtac agtgtgctgc gggtcagatc gacccagaca ttgccgatgg
    11581 tggctgccgt ttgagcggtt aacccgcgaa tgagggcgac caggatggcc atgcccgtgg
    11641 cggcagagac gaagttttgg actgtcaggc cgagcatttg cgtgagataa ctgagcatcg
    11701 actcaccggc ataggcctgc cagttcgtat tcgtgatgaa gctcgaggcg gtgttgaagg
    11761 cgaggtcggg agccaccgcc ccgaaaccag tcgggttcag cggcagatat ccctgcagtc
    11821 gctgcaacac atagagcgcg accaaccccg tgacattgaa caacaacatg gccacggcat
    11881 aggtcttcca atccatctcc tcggttgcac gcacgccgca gacacgataa atcaggcgtt
    11941 cgaagggtcc catcacccgg tccattccgc aggcttggcc cgtatagacg cgtgccatgt
    12001 accaacccag cggcttgacc aacgctatca ggacgaaaaa gaagagtccg atttgagcca
    12061 acccgttgac agtcatcaga accactccgc cttcagcagc gcgatcatga ggtacaccaa
    12121 caaccccagt gacaacactg caccgagcgc gtacatcgca ttcatgacca caccccccta
    12181 gagcaggatt ccgagaaagc ccgccagctt cgttctcgca tcattcagac cctcaacgta
    12241 ccccaagggt acgcctcgga ccttcattcg ctggggcctt gctggacagc ctttttgagc
    12301 atcctgcggt gatgctgttc tattagactg cccctccgga gcaataaatt tcacgtgtga
    12361 caacaaagtt ttcccgtagg ccgctagaga cggtccaacg ccgagatgag ccagccggat
    12421 aacagaaaaa ataccgcggc gatcgtcagg tagatgaggt ccatgtacat gctccttacc
    12481 gggacgtaaa acttagtgtg gcgcgcttcg cccgaaacac ctatccaagc ggtcgccaac
    12541 cggccacatc tcttcacgcc tcttctcttc cactccacac tcaaggtaac gcgcagaaag
    12601 taaaaacggt gtaaaaaggc taagaaaatg gattttctga aaagatcttc ccggacagct
    12661 gttcacgcaa ttggggcgga tcgcttcaga tgacggaaga gtcgaaacac atcaactcgc
    12721 gagggcggcg ctgaagaagg gatgtaggcg aaagtgtgac cgtctattga gcaggtcgag
    12781 aagatttgtt ggatgaacgg gagaggcgtg actgtctccg ggcgctactg atctgaatcc
    12841 gccttatttt tacgtaacgc tcccagcacc gccaggatct ccgccgggcg cggggggcaa
    12901 ccggagatgc gcacatcgac ggggatatgc cgatccaccg gccccgtcac cgcataactc
    12961 cctttgaaca ggccgcaatt cacggcgcag tctcccaacg cgatgacgat cttaggatcg
    13021 gctgtcgcct tgtagacatc tttcaacgca cgctccatat tgacggtcac cggtcccgtc
    13081 acgaccagcg catcggcatg acgcggcgac gcagcaatat ggaccccgaa ccgctccacg
    13141 tcatagaccg gattggccag cgcattcatt tccatctcgc aggcgttgca ggaacccgta
    13201 tcgacttcgc ggatcgtcag cgagcgccgg aacggcttgg ccttttcgat ggcgtcctgg
    13261 ctgaccggct ccgaaggtgc ggctccggca ggatggtgcc ccgtgacgac accggtctga
    13321 agactttttt tgaggattcg aaacatggct cctccctcgg gaacgtaatc agcgagtcag
    13381 taatttaatc gaccattgct agagatcgtt cccagcgtac gacaaattga aactcttatt
    13441 gatcaacgga aagtccggca cgatattgcc gagtgcagcc cattgtattg ccggccaatt
    13501 cacaaaggac ggatcgcgca ctttgcagcg gtgaatccga tctgcgtccc cggccatgac
    13561 gacatacaga atctcgcctc gccatccttc gaccgccgac agcgcccata cacccggctg
    13621 tgcttcgtgc atcggccggg tcacgagccg gcccagtggg agaccgcggc ggacctcgcg
    13681 aatgagacgg atcgactcat ggatctcatc caggcgcacc cgcatcctgg cgcgcacatc
    13741 gccatagcgg tagagggcga ccttcggctg cagcacgtcg tacgcggcaa agggccgatc
    13801 gcgacgcaga tctcgatcga taccggaggc ccggcctacc acaccaatca ccgcatgatc
    13861 ccaggcgatc ccttccgtaa ggatgccggt gttctccagc cgttccgtga gcgacgcgtt
    13921 cgcaaagaca atcttttcaa tcgccgaaaa atcctgttca agggtattca gttccacctc
    13981 cacctgcgcg agctgcaggc cggacagatc gagcgccacg ccgccgacgc aattgacgcc
    14041 acgcagaaaa cgcgacccgg tcaggcgatc gttgagctgc atgagctgct ctttcatccg
    14101 gccgcagtgg gcatgggcca gggcataggc ggtgtcattg cagatggccc ccacatcgcc
    14161 gatgtggttg tgcaaccgct ccaactccag aaacagcgtg cgcagatatc gggcacgggg
    14221 cgagacctcc acgccctgca aggtctcgac ggcctggcaa taggccaggc tatggccgac
    14281 gctcgtatcg ccggagactc gttcggacaa cggtaccgct tcggtcaacc gctgctgctc
    14341 aaacaatttc tcaacgccgc gatgtttcca gaaatggcgc acttcgagct gcatgatggg
    14401 ctcgccggcc accgaaaatc ggaaatggcc cggttcaatg atgcccgcat ggatgggacc
    14461 gacgggaacc tcgaagaccc cctcaccgtg aattttccgg aaagcatact ccccttgcac
    14521 ccgtcctaac acccgatccc agggaaaatc ttttttcagc gggtgggacc ccttcggcca
    14581 atgttcatga cgaacgagtc gacgcagatc gggatggccg accggaatca gtccgaacag
    14641 atcccggatc tcccgctcgt accatttcgc cgcatgaagg tggggagtga tcgatggaaa
    14701 ttcacgctca tcgccatgga gatccgtcgc gagcagcagc cagtctttgc gttccgccaa
    14761 cgtgaagaga tagcagagtt cgtagcgcgc ttcgcgaggg cgatgatcga ccgcccagag
    14821 cagcgacagc gatcctcgca gattcggatt cgtgtgcaga aaatgggcga ccgtcggcag
    14881 atcctgcttc ttcagcctga gcatcgggat gccgtgcacg gcgcacacct cggtcagggc
    14941 ctgcggaaat gccgcttgca tctgctcgac cgccggacgt gcgtcgatca tacggtcacc
    15001 tcaccacaat cacgttgacg gccctggtca gcagcgtctg aatcggatca ggaagaacga
    15061 atccaagccc caacaacgcg acgatcatga tcatcagcgg cacatggccg gcggtccacg
    15121 actctccctg agcgataccg gccgggggag cgccccacac catggccccg acgcgaaaca
    15181 taaacccgcc gaacaacacc acggcaaaaa acagaaacag cgcgacgatg ccgaggctgc
    15241 gcatctcatc cgagatcgtc atcgtcacaa accgtccgac atgcatcgta tcggaggaaa
    15301 agtcttgcgc ggccactgcc gacacaacca gcaactcact gatgaacggc gagaagggag
    15361 gtaagccgac caacgcgcaa cccgccacga gaatcgccac ggcggtaatc ggctgcgcac
    15421 gcgccagccc tcgcaccccg tcgatttcca aggtgtcgaa tcgccgatgg atattgcccg
    15481 cgacaaaaaa ggccagcgct ttggccacgg cgtgattgag caggtgaaag aggccgccaa
    15541 aggtgccgat catcccgccg actccgaagc cgatcatggc cagccccata tgttcgatac
    15601 tcgaataggc aaacagccgc ttgtagttgt gctgaatcag gatgaacaga gaggccacga
    15661 tgaacgagag cagcccgaag gtgagcagca gattgccggt gaactccggg ggaagcgcct
    15721 ggtccaccag cgccttgctg cgcaacacgg tatagaccgc caccgtctcc aacaccccgg
    15781 ccaacatcgc cgcaatcggc gccggcgctt cgctgtaggc atcgggcaac caggtatgca
    15841 tgggaaccag gcccactttc gtgccatacc cgacgagcat gaaaataaac gccagcttga
    15901 gcacattggg gtccaattgg ttggccacgc cgatcagcgc ggtcatattg agcgccgagc
    15961 tcgcatcgcc aaggacccgc acagaggagt aataggtgag caccacgccg aagagcgcga
    16021 gggcgatgcc taccgaacag aggatcagat atttccaacc ggcttcgagg gattcccgcc
    16081 tgcggaaaaa tgcgatcaag aaggtcgtcg cgagggtggt cccctccaac gccacccact
    16141 gcacgccgag gctgttcgcg accgtcgcgg cgaccatcgc aaacaggaac atatggaaga
    16201 gaaaaaagaa gtgaccgaga cgcttcggcg cgatgatgcc ccgggcgacc cgatcgtcca
    16261 tgtaggacca catgtagagc gaacaggaca agccgatggc cgtgatgatc atcagaataa
    16321 agtccgagag cgcatccacg tagacaaagc cgcccaatgt cgtcaccgat ccctcggcca
    16381 gcacctgcct cgtcagcgcc gtctcggcaa cggccaacgc cagcatcgtc gaaaagttca
    16441 cgacgtgcag gacccgggcg cggtggatta ccaggctgag cagaccggcg agtaccggcc
    16501 ccgtcagcag gacgatcacc gaccacatac agcacctact cgccggccat gcgtcatcaa
    16561 ccgactcatt ccttcagcac cgtcagcgtg ctggtgtcta cgctatcgaa cgtatcctgc
    16621 agccgatgcg tgtaaatgcc gacaatcaac ccggccacca acacatcgaa gaagacgccc
    16681 aattcaacga taagcggcat gccgtacgcg gccgccgtgg ctcccaggaa cactccgttc
    16741 tccatgacta gaaagccgac catctgcgtg atcgccttct gccgggcaat catggtgaaa
    16801 aatccgatca gaataatggc cagcgcaatg gccagagaat cgcgcgtcag caggtagcca
    16861 agcggaatga tgggctgcgt gataaagaag gcgatgatca ccaacgcgcc gcagatcagc
    16921 aggcccgccg gaatgttgat gttcatcccc agctcacggg agacattcag acgctcgatg
    16981 accttccgga gaatgcgagg cagcacgatc actttgatga cgatcgtcag ggccgccgcg
    17041 aaataaatgt gcttatgtcc ggtcagaaac gcaacgagcg cggcagtcgc cgccagaaac
    17101 gcggactgca gcgcaaacag atccacacag gccgacaatc gacgctgcgc cacgatggca
    17161 aaacaacaga gcagcagcag gaccgagcag agatcgacga gttgagagcc caattgggtg
    17221 gaagccggca ccgcattacc ctttcaacgt atagaagaag accagcgcga gcagcgacag
    17281 aatgaacgcc gcgcccagca tttcgggcac ccggaacaat cgcagcttcg cgaacatcga
    17341 ttcgatcaca ccgatcacaa gagccagcgc agccaccttt ccgagataca ccaggagacc
    17401 gacggcgatg gctgacggcg ccagactcgt ggcgataccc cagggtgcaa agacattcac
    17461 gatcaaggtc aggaacacca gcaacttgat cccggccgcc cattccatca acgccaggta
    17521 ccgcccggag tactcgagca gcatggcctc gtggatcatc gtcaattcca ggtgcgtcgc
    17581 aggattgtcc accgggacac ggccggtttc cgccagcgtc acgatgaaca gagcggccag
    17641 cgccatcaaa tgcgcaggtg gggccacgac gaccccttcc atcaacgcgg actgatgcac
    17701 gatggtgctc aaattcgtgg agccggcggt cagcgcaatg gaaaggatag acaacatcat
    17761 ggccggctcg gccagcgtcg cgacaatcgc ctcgcggctg ctgcccatcc ctccgaatgc
    17821 cgatcctgca tcgagccccg ccaggatcag aaagaacgtc cccaacgcca gcagatagac
    17881 gagcgcaatg atgttcccgg caaaattgag cggtgtgtga gagacgaata ccggcatcaa
    17941 caaccccgcc gctacggtcg aggcaaagac aatgtacggc gccgcggtaa aaatccagga
    18001 ggtcgtcgaa gagatgacgg gctgcttccg gaacagcttg gccagatcgg cgtagggctg
    18061 aaacagactc gccccgcggc ggcactgtag acgtgctttg acctttcgga tcaggccgac
    18121 gacaaacgga gagagggtca acaacagcac gagctgaacg acgaccagta cgatgtggat
    18181 gatcgactgc atagctcccg tcactcaatg atcgaacgac taggcacgac acatacacac
    18241 atcccgccgg ctctccctac cccgcccata acaacaacag caccagcgtc acgaaaatgt
    18301 aggtgagata cagatggaga ctgccggcct gaatgatgcg cagccggtcg gctgtcgcca
    18361 gcagcaatgt caccaccggc cgatagaggt atttctcgaa gaccggttca atgtgcgact
    18421 cgaaacgctg atgtttgatg aagtacttcg attgctccag aaactcctgt tccagcttca
    18481 ccgtcggctg gtagatggtt tcaaagacct gcttgatcgg ttgcgcgaag ccggtcgcgg
    18541 tgtattccat gcgaggcgtc agattcagcc cgcagcccca ggtcttgtaa tagcgcgcgc
    18601 gcagcccctt cccgcagagt cgggcaatca gcaaccccaa cgcccccgcc ccgaccaaga
    18661 gcatggcgag caccggcgtc gagatgctcg aaaactccac cgtcaccggc gccaccaccc
    18721 agccgtcgag cgccagcacc tgtgaactga tggccgcacc ggtcaatgga gccgtgaccc
    18781 gatccagcaa cggaacgacc agcatgggcg ccagccccag cacaatgcag agccctgcta
    18841 aaaagcccat cccaacccgc atcgaccagg ggacttccgt cgcatggcgg gcatgggcgc
    18901 tgcgcggctg cgccagaaac gacatcccga aagccttcgc aaaacaagcc agcgccagcg
    18961 cgcccgtcaa agccaacatc gcggcggcaa tcggcaacat caacttgaga aacagggtcg
    19021 gcagttggaa gctgagaaac aaactctgaa agaccagcca ctcgctgacg aaaccgttgg
    19081 tgggcggaag cgcggcgatg gagacggcgc cgatcaggaa aaaggcgccc gtccagggca
    19141 tgcgccggag caagccgccg tactcctcga tgtgtctcgt atgggtggag aactgcaacg
    19201 ctccggcgcc cagaaacaac agcgccttga acgccgcgtg gttaatagtg tggtacaggc
    19261 ccgccagcaa tccgagggca gccagttcat gcaagccata ggtgtggaag atcatccctg
    19321 cgccgattcc cagcagaatg atgccgatgt tttccacact atgaaacgcc agcaggccct
    19381 tcaggtcatg ttccatcaag gcatacatga cacccaggag cgccgacacc gtaccggcga
    19441 ccaacaacgt gaagccccac caccagggga ataccccgcc catgaaatcg aagtacaccc
    19501 ggatcaaccc gtagatggcg gtcttgatca tgacccccga catcagagcc gagatgtggg
    19561 acggggcggc gggatgcgcg tagggcagcc acacatgcag cgggacgatg ccggccttcg
    19621 cgccgaatcc gatcaaggcc gtgaagaacg cgatcgatcg caatccctca ggaagttgct
    19681 ggtcggggtg gcgaaacgcc tcgaaggaga atgaaccggt ttcctgggaa aagatcaaca
    19741 acgtgaggat gatgaacgcg gtgccgacgt gcgtcatgat gagatagaac aacccggcct
    19801 cgcggacgcc gggcttctcg tgctccgtca caaccagcac ataggacagc agggacatga
    19861 cttcccagag gatcaggaag aagaacccgt tgtcggccag gaccaccagg gtcatggaga
    19921 gcaaaaaccc gttgaggagg gagcccaaca atcctaccga gacccggcca gaaaactcct
    19981 cgacgtagcc ggacgcgtag atggacgccg ccacccccac cagcgagatg gtcaggacaa
    20041 aaaacgctgc cagcgggtcc agacggattg cgaaggtcag atggggcatg ttggacgcaa
    20101 gcgagagagt cagaggagcc gttgagagga gccccgcaac acccagcaca ataccgacga
    20161 cacccgcggc agccgcacag ccatgggcaa aaagattctg aagatgggga cgtgttgtgc
    20221 agaggggcag cagccccccc agaaggtacc agacgagaag aacgctcagg agggcggtca
    20281 tcacgacact accctcgact cacgcgcagc atcgcgatgc tcaccactta ctcaagagat
    20341 acgtcagctc ttgcatttgt caaagtacgg aagaaacccg tcgtcttatt tactcatgta
    20401 tcaccttccg tcgttcgacg caacttgcgc acgacagtcg aacgctgcgc cactgctcgc
    20461 gggcactccg tctgtaggaa tatgtgcgga gaactatgac agcttgtgga ggggcgtgag
    20521 aagagtcggc aaggcgttac ttgcgcacgt ctcccagcac acaccagaga cggcgcatgc
    20581 cggatttctg atcggtcacg gcctggccgg agaagaaact ttgctgccag gccacgatgt
    20641 cgtc
//
LOCUS       DAORRQ010000070        20316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_158_length_20316_cov_135.348058, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000070 DAORRQ010000000
VERSION     DAORRQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20316
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_20316_cov_135.348058"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PK224_17005"
     CDS             complement(<1..330)
                     /locus_tag="PK224_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17498.1"
                     /translation="MKTMGNAVGAGLIMTVAIMTATAQAENSIIPADMVYVGQGPSIM
                     GLDKAQPADSGRRLTLYDKRMKTPLSAEAFNDEGPAHTVFLDSYLIDKYEVSNKDYGA
                     FIKATGHP"
     gene            complement(396..1358)
                     /locus_tag="PK224_17010"
     CDS             complement(396..1358)
                     /locus_tag="PK224_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17499.1"
                     /translation="MRRHIGLQIGIGAVLVAGSMSMAVSAKAAGDHKDMVLIPSGEFT
                     MGSHEHSDEARHQVVLDAYLIDKFEASNTRYKEFMRATGHPAPAYWDDPRLSKPEQPV
                     VGVSWTDANAFCKWDGKRLPTEAEWEKAAKGPEGDNHYPWGHHLDPKKANYGQNVGRT
                     TPVDSYPEGVSGYGVYNMAGNVFEWVDDWYDPKYYRTSNALNPRGAEKGYNFANQGPV
                     KVLRGGSWLAPETSLHTSHRFWNQPENNSYGVGLGFRCAKSASMVSDEAAQAGRDAFI
                     QALVGMGAEKNADALASIEKALAADPDNKEYLATRDLIKKSMKK"
     gene            complement(1416..1712)
                     /locus_tag="PK224_17015"
     CDS             complement(1416..1712)
                     /locus_tag="PK224_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17500.1"
                     /translation="MATTKTMEGTVAVSEIRRQILTLHQESNCLRCGGLMVRDFCTDL
                     LSGASGLDSSTSRCVQCGDVVDPVIRWNRHLQQAAGVVREMKTSKPALQGQVSV"
     gene            1850..2767
                     /locus_tag="PK224_17020"
     CDS             1850..2767
                     /locus_tag="PK224_17020"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRB17501.1"
                     /translation="MNTHRIHRVAKLTGLSRDVIRVWERRFGLLKPTRGANRYRNYSD
                     EDVALLRYLKQQLDAGASIGDLAKLGREELITRLRAEAPRTAVVDNTFDRLLRELLSA
                     LEPFDRVTFEKRLNGAVAVVPFEEALHGILLPLQERVGDLWHSGRISIALEHYVTSQI
                     EQKLYAAMNQLPVAEFGAKVVVACPPGEEHDIAALAVAYRCRVRGCRVYYLGANVPIS
                     SLSRLCREVAPDLAILSLTLVLQEGNAAELIHSLAEELKPVTTVMAGGHGARVMRDQL
                     AQYRIDVLDTFSELDEVLDRFTRRFTIPG"
     gene            2786..3202
                     /locus_tag="PK224_17025"
     CDS             2786..3202
                     /locus_tag="PK224_17025"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HRB17502.1"
                     /translation="MAVYPLTVFYDGACPICAREMALMKRLDRTQRLTLLDFSLAGFD
                     AAPAGLAVADLSAVIHAQWADGTVITGVEVFRAIWEAVGLGFLSRLSRLPMVAPLMTK
                     AYGWFARNRLWLTGRNNACPGNACAAASPKPLRASQ"
     gene            3559..4020
                     /locus_tag="PK224_17030"
     CDS             3559..4020
                     /locus_tag="PK224_17030"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRB17503.1"
                     /translation="MTLRVSLIVLFSIGGILLLVGYWAGQYHPDLLPHTFSLQTAPPD
                     PSQGQPGVTINLPPVVAPVDDGQRYYYVQVNLALELDRSGTAGLIQARHDAIDRQVME
                     ILHTYAVSDLRATGQLPALRADIRRAINKLLPKGQVQNVYITNWLMTPVGY"
     gene            complement(4121..5491)
                     /locus_tag="PK224_17035"
     CDS             complement(4121..5491)
                     /locus_tag="PK224_17035"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRB17504.1"
                     /translation="MKPSHTLVDVPGGEPLLGHLRAFRRQPLQAMSQWWRRHGDALRF
                     RLGPKTLYLLSHPDLAEEILVHQADCFVKVYEPRRPTGLALVLGNGLVTSSGDVWKRH
                     RRIIQPVFHRARMAAMAERMAQVGEQRIAGWTVHAGGPVDIADEMMRLALEVISQTMF
                     HTNVADHIDHISHALRVSLKYAFDSFHSPLRLPVWVPTPRNREFRLALQFMDKLIYGL
                     IAERRRTGAQRDDLLDLLLRARDEETGAGLSDQELRDEALTIFAAGHETTANALAWTW
                     YLLATHPEVKACFHQEVDRVLQGRTPQADDLQQLPYTRAVFDEAVRLYPPVPAVQRKA
                     ATRTSIGGLTLPAGALVLVGIYHLHRHPAFWRDPERFMPERWLEGERPASRCAYLPFG
                     AGPRACVGTHFATVEGPLLLALIGRSYDLQLAQEHVEPEIMVTLRPKHGIRMTIQPRH
                     QPVHSA"
     gene            complement(5677..6813)
                     /locus_tag="PK224_17040"
     CDS             complement(5677..6813)
                     /locus_tag="PK224_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB17505.1"
                     /translation="MNIKEFKSGGYEQQYEYRSFLPTPINREWVVSDPQILTLLEETN
                     RLLGELNGFSQLIPDVDFFIRMHITKEATTSSRIEGTQTNMEEALIGKLDVDPEKRDD
                     WQEVHNYIQAINFSIKQMDQMPLSNRLLRETHAVLLRGVRGTHKQPGEFRTSQNWIGV
                     SLNHATFIPPHHLHVPDLMSDLEKFMHNDQIHVPHLVRIAFIHYQFETIHPFLDGNGR
                     LGRLLIVLYLVGSGLLHKPALYLSDFFERHKGEYYDQLMAVRTTQKMEPWLRFFLLGM
                     RETTALSIQVFRDILTLKERIERKYMPTFHVRRQANAQMLLKSLYRAPVINIKMVTDL
                     IKTQTNTAAALIDDFVKLGILRELTGQRRNRLFIFQDYVRLFKK"
     gene            complement(7060..7146)
                     /locus_tag="PK224_17045"
     tRNA            complement(7060..7146)
                     /locus_tag="PK224_17045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7110..7112),aa:Leu,seq:cag)
     gene            complement(7236..8711)
                     /locus_tag="PK224_17050"
     CDS             complement(7236..8711)
                     /locus_tag="PK224_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17506.1"
                     /translation="MTAIPDTLPDLLDQLAATLDGDAARGTEQGLDQSIPLTRGRLLQ
                     TVGNLHLYEFYLSADVVVGADLSATLLLGEEAEPTEGIVLFQDGERLVLQLFDAIGDV
                     VPSATLVPDASGLALTTSRRLAEMSKTGASYTLGPAERLLPVLEAGQTGDRAALEALV
                     PTSVLTPLWQDDLTARRQKLATLIIELLRGNKRILLVAPDHESADAITLLTARAMKAA
                     GLTFKSWLSRYEVATSKESGGIPLPDLGFEAQMHQFYAKSRSDKAVLRKKYDRFRELT
                     PILAYKAQKQKDLDEVRLLEWRLVTQLTDFLAKIKEIDQTLTDYEQLPIWKRLSMQAV
                     GKNVESLREYKAIYERHCAGLRHELDIAKERIAELIPEATVPKELKPEFAELKEEVIR
                     LGGTKKIRELLAAQEDTNRQAFVQNRRVIVTTAARVAADPLFRKVRFDVLIADEAPRI
                     RAAFLLAAAGLVRERIVLSGDLREVTAGGAWNLTDGVLTAG"
     gene            9013..9507
                     /gene="rplI"
                     /locus_tag="PK224_17055"
     CDS             9013..9507
                     /gene="rplI"
                     /locus_tag="PK224_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRB17507.1"
                     /translation="MKVILQETLEGVGDLGDLLDVSNGFARNYLLPRKKAVEANSRNI
                     KEFEHAKRAAAEKAKKEKQDIEAHGKKISAVSLTVAAQVGKDDKMFGSVTAKDIAEGL
                     AEQGFTVDRRKIQLAQPIKELGTFAIAIKLPREVTATIAVHVVKKQDEQEAQAEPEEA
                     APTA"
     gene            9562..10839
                     /gene="glyA"
                     /locus_tag="PK224_17060"
     CDS             9562..10839
                     /gene="glyA"
                     /locus_tag="PK224_17060"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRB17508.1"
                     /translation="MQELIGSLDALKSTDPETYEAIVAEEQRQRDKLLLIASENFASP
                     AVLAAQGSVMTNKYAEGYPGKRYYGGCQHVDTVESLAIERAKQIFGAEHVNVQPHSGS
                     QANMAAYLSVLKPGDTILGLDLAQGGHLTHGSKVNFSGIIFRAFSYGVDRQTETIDYA
                     AVQKIAEQCRPRMLVVGASAYARTLDFPKFQEIAKSVGAYLLVDIAHIAGLIAAGLHP
                     NPVPYADFVTTTTHKTLRGPRGGVTMCKAEYAKAVDKIIFPGLQGGPLMHVIAAKAVA
                     FKEALSPAFKRYQQQVLANARTLAQGLVDRGYKIVSGGTDTHLMLVNLTNKGITGKEA
                     DAALDAAGIIVNKNAVPYDEKPPATASGIRIGSPIVSTRGMREAEMREIVALIDRVLQ
                     HPQDQQVQAEVRAQAKTLCNRFPIFHAYDSSPA"
     gene            10855..11319
                     /gene="nrdR"
                     /locus_tag="PK224_17065"
     CDS             10855..11319
                     /gene="nrdR"
                     /locus_tag="PK224_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRB17509.1"
                     /translation="MKCPFCDDVEDKVVDSRMAKEGEVIRRRRECLSCKRRYTTYERV
                     EETMPAVVKKDGRREPFDRSKIVSGLKKACEKRPISTATIETVTDRIEKRIQDLGETE
                     IVSTAVGEEVMRELSQLDQVAYVRFASVYREFKDIDQFMDEIKALAQQRRER"
     gene            11427..12548
                     /locus_tag="PK224_17070"
     CDS             11427..12548
                     /locus_tag="PK224_17070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRB17510.1"
                     /translation="MATTKVTRNRRTAKRPSRRRSPGATHVVIIGAGRGGTALMEIFA
                     NDPLVRIVGVADISDQAPGLGLAKRLHIRVTRNYRQLLKMGPVDLVIDVSGNPEVGEY
                     LQDIRRMGVSVIGGASAKFMWQLIEARIRATADIEKALNKYQSLYRLYVKETGAAVTE
                     ERTRIACEIHDGLVQSLAGVNFKLELSQELIRKNPKASLATIRESKAQLKLAIQEARQ
                     VIFNLRPLHYDKMELIPALTNYLKSYETQYHIKTAFSVTGDETILFPRTKIFLFRIVQ
                     EALSNVQKHAKAGRVSVQLDIRFDLLQVTISDNGIGFDMDAVLRDPEKWDHFGIRGIL
                     ERARLVGGEATIDSKKGRGTRIVLRIPLADKETIRHGKN"
     gene            12535..13206
                     /locus_tag="PK224_17075"
     CDS             12535..13206
                     /locus_tag="PK224_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17511.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKDDINVVGEAQDGVEAVEK
                     TKTLMPDVILMDVSMPRMGGIEATRQIKREFPHMGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMSQKKGKKPAWVEHDLTER
                     EITVLRLVADGKTNKEIANNLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LEPRP"
     gene            13490..14416
                     /gene="lpxC"
                     /locus_tag="PK224_17080"
     CDS             13490..14416
                     /gene="lpxC"
                     /locus_tag="PK224_17080"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRB17512.1"
                     /translation="MRFQQTIGSPVSCSGVGLHSGQPVTLTLRPAPPNTGIVFVYRNG
                     SEETLLPAAVSNKVPTELCTAISVNGRQVKTIEHLLSALVGMEVDNVYAEVDAGEVPV
                     LDGSASPFVRLIRAAGVIPQTRRQSYVKITQPIEVVDGARRVRIEPSSTPKITYSIHY
                     DHPLIQTQSYTYACSASAFEQDIATARTFGFLHEVEALWARGLGKGGTLDNTVILSKD
                     GVVNESGLRFQNEFVRHKVLDLIGDIALLGFPFIGHIVAERSGHAMHTRLVEQILAQR
                     DKWALITGEHAVAASESRSSLGLLRPAPSLAI"
     gene            complement(14661..15170)
                     /locus_tag="PK224_17085"
     CDS             complement(14661..15170)
                     /locus_tag="PK224_17085"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRB17513.1"
                     /translation="MEELTDILVGLEQRISAYRNRYQELEKKRRRLDDEIAMIKKYLE
                     LAETLYRVEADKAKLASLSSQIITDEKGIRPLPVTDVTDQSREILLGKSKYVGKSVPE
                     AAYQILREASRPMHAKELLQRLLEGGLQIKGKTPLTSVATSLKRDKRFQKVGPNTFAV
                     MTQELTAVV"
     gene            15421..16242
                     /locus_tag="PK224_17090"
     CDS             15421..16242
                     /locus_tag="PK224_17090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRB17514.1"
                     /translation="MQTSDRPSPEDRSSFLATAIRAAEAAGTVLLEHARSGFRIDYKA
                     AINLVTDADRGAEESIVRTILSAHPSHRILAEERGEDGATDSPYRWIIDPLDGTTNFA
                     HGFPFYSVSIGLECDGECIMGVVLDPVRRELFTAVLGQGAYLNGERLRVSRIETLEHS
                     LLVTGFAYNIRETTNNNLDHFSRISLRAQGVRRTGSAALDLSYVAAGRFDGYWEVKLS
                     PWDMAAGVVILREAGGVVSGFSRDRFSLYGQELVATNGHIHEHLLRAITQHPDPH"
     gene            16386..16655
                     /locus_tag="PK224_17095"
     CDS             16386..16655
                     /locus_tag="PK224_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17515.1"
                     /translation="MASQVPPQKPVTPAANPQDVWDVELLHVHVSRKGQLVNAEWALH
                     PQIKADLSAEEWKELGELMSKVTTIVGHRFSQALGEADPAPPGNA"
     gene            16745..17077
                     /locus_tag="PK224_17100"
     CDS             16745..17077
                     /locus_tag="PK224_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HRB17516.1"
                     /translation="MDCYYHSKDLGKFGDMGKGNPVLWEKFMSYYSAVFAEGALTERE
                     KALIALGVAHAVQCPYCIDAYTQACLEKGSNTEEMTEAVHVACAIRGGASLVHGVQMR
                     NVTEKLSM"
     gene            complement(17114..18979)
                     /locus_tag="PK224_17105"
     CDS             complement(17114..18979)
                     /locus_tag="PK224_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HRB17517.1"
                     /translation="MSSSQAQVALVNMPFSFSKYPSIQLGTLSALLKAKGIGVECHHL
                     NVRFAHKIGIPLYESICEKRALFGEWLFSYLLFRDNPQRAEYPRVFKPVFEQIARESG
                     QPISFFEEMATRTAPQFLTGAMTGIDWGQYKIVGFTSTFDQNVASLTMAKLIKDLYPD
                     VKIVFGGANYDGEMGLEYFRAFPFIDHVVVGEGEVTFPALVTHILSGSAGPCPKGVTY
                     REQGDIRCEPNTALFTEFAQTGPPDYDDYYHLLAELGTEASRGLDRILLYEGSRGCWW
                     GEKHHCTFCGLNAQSMKFRAKSSEQVAREMAYLSSRYDTTRFRLVDNIIDMKYVENLF
                     GRFAAEHCDLDVFIETKSNLQKSQIRTLAVGGVKCMQPGLESLSLSQLKAMDKGVTPM
                     QNIICLKWSCYYRVSVSWNILLGFPGETNEDYRRQIDLLPSLFHLQAPEATGKFWLQR
                     FSPYFTRPHEYGVRIMGPGTAYEYVYDAVRVDLRKIAYDFEYELDDWPVDPHVYQELI
                     GLVEEWQRLARSSDRPFLYYSKAMEYVTIYDGRNPQMPTRRRYDWPAAGIIEACNEAA
                     KSRVQIRAALSEATRDMACSEDELTEALGILTSRRILYEERGKYFTLAIPEHPYY"
     gene            complement(19194..19541)
                     /locus_tag="PK224_17110"
     CDS             complement(19194..19541)
                     /locus_tag="PK224_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRB17518.1"
                     /translation="MSLADNKCIPCRGGVPPLPADRTQTLLQEVGRGWSLNAQGHLER
                     LYTFKDFAQSLAFANKVGAVAEAEGHHPDLYVAWGKCKVEIWTHKINGLTESDFYLAA
                     KADREFEPFRAGA"
     gene            complement(19628..20116)
                     /locus_tag="PK224_17115"
     CDS             complement(19628..20116)
                     /locus_tag="PK224_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB17519.1"
                     /translation="MLVQDVMSTGVVTARRNESVRSVVTKMLSRHCGAIPVVEDGELL
                     IGMVTLRDVLIPLYPNYGEYIHDNVHSRDFVEMEEGYADVLTQRVEEVMSLNPLTVTP
                     RTPVLEAASYMGLKNFRRIPVVEKGTLVGMVSVGDINRGLFFERGHAVMDEHRVAQPS
                     GR"
BASE COUNT         4371 a         6149 c         5678 g         4118 t
ORIGIN      
        1 tggatgaccg gttgccttga tgaaggcgcc atagtctttg ttcgacacct cgtacttgtc
       61 gatcagatag gagtccagga acaccgtgtg cgccggccct tcgtcgttga acgcctcggc
      121 cgacaagggc gtcttcattc gtttgtcgta cagcgtcagc cggcgccctg aatctgccgg
      181 ctgggccttg tctaatccca tgatggacgg tccctgtcct acgtagacca tgtctgcagg
      241 aatgatggaa ttctccgcct gtgccgtcgc cgtcatgatg gcgacagtca tgatgagccc
      301 tgccccgact gcgttcccca ttgtcttcat gccacacctc ctgcccctga gggccgggcc
      361 gggggacggt cacccgcccc ctccacggtt atgtgctact tcttcatgct cttcttgatg
      421 agatcgcgag tcgccagata ctctttgttg tcgggatccg ccgccaacgc cttctcgatg
      481 gaggcgaggg cgtcggcgtt cttctccgct cccatgccga cgagcgcctg aatgaaggca
      541 tcacgcccgg cttgagcggc ttcgtcggag accatgctgg ccgacttcgc gcaacggaaa
      601 cccagtccga cgccgtagga gttgttttcg ggctgattcc agaaccggtg gctggtgtgc
      661 agcgaggttt ccggagccaa ccaggacccg ccacgcaaga ccttcaccgg gccttgattg
      721 gcgaagttgt agcccttctc ggcgccacgg ggattgagcg cgttgctggt gcggtaatat
      781 ttcgggtcgt accagtcatc cacccactcg aagacgttgc cggccatgtt gtacacgcca
      841 taaccgctca cgccttcagg ataggagtcc accggcgtag tgcgtcccac attctggcca
      901 tagttggcct tcttcggatc gaggtgatgc ccccacggat agtggttgtc tccctccggg
      961 cccttggcgg ccttttccca ttccgcctcg gtgggcaatc gcttgccgtc ccacttgcaa
     1021 aaggcgttgg cgtccgtcca actcactccg actaccggtt gctcgggctt gctgagccgc
     1081 ggatcgtccc aataggccgg tgccggatga ccggtggccc gcatgaactc cttgtaccgt
     1141 gtgttcgacg cttcgaactt gtcgatcaga taggcatcga gcactacctg atgcctcgcc
     1201 tcgtcggaat gttcgtgact gcccatggtg aactcgccgc tcgggatgag gaccatgtcc
     1261 ttatggtcgc cggcggcctt cgccgacacc gccatcgaca tggatccagc tacaagcacc
     1321 gctcctattc caatttgaag acctatgtgc ctccgcatga gaaacctcct tggttgagaa
     1381 ccctgacaca acccccagtg gggtcacgat tgttgctaaa cagagacctg gccctgcagg
     1441 gccggcttcg atgttttcat ttcccgtact acgccggcgg cctgctgcag gtgccggttc
     1501 catcggatga ccggatcaac gacatcaccg cattgcacac aacgcgaagt ggaggaatcg
     1561 agcccgcttg ccccgctgag caggtccgta cagaaatcac gaaccatcag tccgccgcac
     1621 cgcaggcagt tcgactcctg gtgaagagtc agaatctgtc ttcggatttc acttactgcc
     1681 acagttcctt ccattgtctt tgtggtcgcc atgatcactc ctcgcatgtt catgtccaat
     1741 gtttgcaacc tctatgtcca atgtttaacc ttttttgtcc aatgtgtcaa tagttatttc
     1801 aataaatatt tgacaaacat tggacacaac catatacttg ggctcaacaa tgaatacgca
     1861 cagaattcat agggttgcga agctcaccgg tctgagccgc gatgtcattc gggtgtggga
     1921 gcgccgattt ggcctgctga aaccgacacg aggcgcgaat cgctatcgca attattcgga
     1981 tgaagatgtg gccttgctgc gctatctgaa gcagcaactg gatgcgggag cttcaatcgg
     2041 tgatctcgcc aagctggggc gtgaggagtt gatcacccgc ctgcgcgcgg aagcgccgcg
     2101 caccgccgtg gtggataata cgttcgaccg actcttgcgg gaactgctct cggccttgga
     2161 accattcgac cgcgtcacat tcgagaaacg cttgaacggc gcggtcgcag tcgtcccgtt
     2221 tgaagaggcg ctccacggaa ttctgctccc gctgcaggaa cgcgtcggag acctgtggca
     2281 cagcgggcgc atcagtatcg ccctcgaaca ttacgtgacc agccaaattg aacaaaaact
     2341 gtatgcggcc atgaatcaat tgccggtggc ggaattcggc gcgaaagtcg tcgtggcctg
     2401 cccgcccggt gaggagcacg acattgcggc attggcagtc gcctaccgtt gccgcgtgcg
     2461 tggatgccgg gtctactact taggagccaa cgtgccgatc tcctccctca gcaggctctg
     2521 cagggaggtc gccccggatc tggccatcct gtcgttgacc ctggtgctgc aggaaggcaa
     2581 cgcagcggaa ttgattcact cgctcgcaga ggaattgaaa cccgttacca cggtcatggc
     2641 cggcggccat ggcgcgcgcg tgatgcgcga tcagcttgcg caataccgca tcgatgtcct
     2701 ggataccttc agcgagttgg acgaagtgct ggatcgattc accaggcgat ttacgatacc
     2761 gggatgacag ggaggcaccg ttcggatggc tgtctatcca ctcaccgtgt tctatgacgg
     2821 agcctgcccc atttgcgccc gcgaaatggc gctgatgaag cgtctggaca ggacacaacg
     2881 cctcaccctg ctcgactttt ctcttgccgg cttcgatgcc gcaccggccg gcttggctgt
     2941 cgcggatctg agcgcggtga ttcacgccca gtgggcagac ggcaccgtca tcaccggtgt
     3001 cgaggtcttc cgtgcgattt gggaggcggt cggcctggga ttcctgtccc gcctcagccg
     3061 actcccgatg gttgctccac ttatgacgaa ggcctatgga tggtttgcca gaaaccggct
     3121 ctggctcacc ggccgcaaca acgcctgtcc gggaaatgct tgcgccgccg cttcacccaa
     3181 gccactgcga gcctcacaat agtccccgtc ctctctttcc caacgcgcca gtcatgacaa
     3241 agcacggcga agccacgagg cccggatcag gccttcattc cgagcccggg catgttccca
     3301 ctcggtcgaa catggcatcg tgcgaaggaa aagtctgaca gaggcgccct acgcaaccat
     3361 ccgaacctca accaacggca ctaaccatca cgaaccacca ggcctgggca atttcctagt
     3421 gtctacccgg gaagtccctt gctgacaatt ttcaaggaga tgctacggtt attcagaacg
     3481 tcctcatggt gcgagaccgc gtcagcagtg tcatcgggaa caggaccgcc gcttcacgtc
     3541 acattcatcc ccttcagcat gacattacgc gtcagcctca tcgtgctgtt cagcattggc
     3601 ggtatcctac tcctggtagg ctattgggcg ggccagtacc acccggacct cctgccgcat
     3661 acgttctcat tgcaaaccgc tccccccgat ccctcacagg gacaaccagg tgtgaccatc
     3721 aacctgccgc ccgtcgttgc gcccgtggat gacggccaac gttactatta tgtgcaggtc
     3781 aatctggcct tggagctcga tcgctcgggg accgccggat tgatccaggc ccgccacgac
     3841 gcgatcgatc ggcaggtgat ggaaattctt cacacctatg ccgtcagcga tctgcgtgcg
     3901 accgggcagc tgccggcctt gcgagcagac attcgacgcg ccatcaacaa gttgcttccc
     3961 aaaggacagg tccagaacgt ctacatcacc aactggttga tgactccggt cgggtactga
     4021 accgaattct gcaagaaata cgactgccga cggatcgacc ttccacccct agtgtttctg
     4081 aaagacagag agagccagtc cgaatgaagc cccgcttgcg ttaggcggag tggaccggct
     4141 ggtgtcgtgg ctggattgtc atgcggatgc cgtgtttcgg acgcaaggtc accatgattt
     4201 caggctcgac gtgttcctgc gcgagctgca gatcgtagga gcggccgatc aacgccagga
     4261 gcagcggccc ttccaccgtc gcgaagtgtg tccccacgca ggcccggggg cctgcgccga
     4321 acggcagata ggcgcatcgg gaagccggcc gttcgccctc cagccatcgc tccggcatga
     4381 atcgctccgg atcgcgccag aaggccgggt gtcggtgcag gtgatagatg cccaccagga
     4441 caagcgcccc ggcaggcagg gtcaacccgc cgatgcttgt gcgggtggcg gcctttcgct
     4501 ggaccgccgg aacgggggga tataaacgaa ccgcttcatc gaaaaccgcg cgagtgtacg
     4561 ggagttgttg aagatcgtcg gcctgaggcg ttcgcccctg cagcacccgg tccacctctt
     4621 ggtgaaaaca cgcctttacc tccggatggg tggcaagcaa gtaccaggtc caggcgaggg
     4681 cattggcggt ggtctcgtgt ccggccgcaa agatggtcag cgcttcatcc cgcagttcct
     4741 gatcgctcaa cccggcgcct gtttcctcat cgcgggccct gaggagcaga tcgaggagat
     4801 cgtcgcgctg cgccccggtg cggcgtcgct cggcgatcaa tccatagatc agcttgtcca
     4861 tgaactgcag agcgagacga aattcacggt tgcgcggggt cggcacccac acgggcaggc
     4921 gcaggggact gtgaaacgaa tcgaacgcat acttcagact gacccgtagc gcatgactga
     4981 tgtggtcgat atggtccgcc acgttcgtgt gaaacattgt ttgagagatc acttcgaggg
     5041 ccagccgcat catttcgtcg gcgatatcga ccggcccccc cgcgtggacc gtccaacccg
     5101 cgatccgctg ctcgcccacc tgcgccatac gctcggccat cgccgccatg cgggcgcgat
     5161 gaaaaaccgg ctggatgatg cgccggtgcc gcttccagac gtcgcccgag ctggtcacca
     5221 atccgttacc caagaccaac gcaaggccgg tgggacgccg cggctcgtac actttcacga
     5281 aacaatcggc ctgatggacg agaatctctt cggcgagatc gggatggctg aggaggtaga
     5341 gcgtcttagg accgagccga aagcgtagcg catcgccgtg ccggcgccac cactgcgaca
     5401 tggcctgcag cggttgccgc ctgaacgcac gcagatggcc caacaggggc tcgccgccgg
     5461 gaacatcgac cagcgtatga gatggcttca catcgacagg cattgagtga ggtgagacgg
     5521 ttgattgttg gacgcgccgg gcattggatc tcacaaggac tgttccgcat cgtacaaact
     5581 tcgcgtgaag agtggtaggg ccgaaccacc agggctacat ggcgtacagc tggctggcac
     5641 ttgcgttggt aacgttccta ccgtgtgaat ctggcgctat tttttgaaca ggcgcacata
     5701 gtcctgaaag atgaacaggc gattacgcct ctgtccggtc agctcacgaa ggatgccgag
     5761 tttgacgaag tcatcgatga gcgcagcggc ggtattggtt tgtgtcttga tgagatcggt
     5821 gaccatttta atgttgatca ccggcgcccg gtagagagac ttcaagagca tctgcgcgtt
     5881 agcctggcga cgcacatgga aagtgggcat atatttgcgc tctatccgtt ccttgagtgt
     5941 aaggatgtcc ctgaacactt ggatggacaa ggcagtcgtt tcgcgcatgc ccagcagaaa
     6001 gaatcgtaac cacggttcca tcttctgagt cgtacgcacg gccatcagtt gatcgtaata
     6061 ctcgcccttg tgacgctcga agaagtcgga tagatacagc gcaggcttgt gtaagaggcc
     6121 ggagccgacg agatagagca caatgagcag acgacccaac cgtccgtttc cgtctaaaaa
     6181 cggatggatg gtttcaaact gataatggat gaacgcgatt ctgaccagat gcggaacatg
     6241 aatctggtcg ttgtgcatga acttttccag gtcactcatg agatccggca catggaggtg
     6301 atgcggcggg ataaaggtgg cgtggttaag actgacaccg atccagtttt ggctggtgcg
     6361 aaattctcca ggctgcttgt gcgtgccacg caccccgcgc agcagcacag catgagtctc
     6421 tcgcaagagc cgattggaca agggcatctg gtccatctgt ttgatcgaaa aattgatggc
     6481 ctggatgtaa ttatgcacct cctgccagtc atcgcgtttt tccggatcga cgtcgagctt
     6541 gccgatcagc gcctcctcca tattggtctg agtgccctca atacggcttg aggtcgtggc
     6601 ttccttggta atgtgcattc ggatgaaaaa atccacgtcc gggatgagct gagaaaaccc
     6661 gttcaactct ccgagcagac gattcgtctc ttccaggagt gtgagaatct ggggatcgga
     6721 cacgacccat tcccggttga tcggcgtggg caggaagctg cggtactcgt actgttgttc
     6781 gtacccccct gacttgaact ccttgatgtt catgaaactc ctcgactcct gccgggccaa
     6841 tttgatctaa gcggaccgtt atgtcaaatg catagcgcca aattgatata acgcagaacg
     6901 tatgtcaaat ggccggaatt gatagataga aggagttcgc ggtcgagcaa gctactcgga
     6961 aggccaggaa aagagaggag aagagagaga aagactgcta gagggttttt cccgtcgccg
     7021 agagccatcg gaagggcttc gggtcgagca agctgtgtat ggtgccgaag ccgggagttg
     7081 aacccggata cccttacggg cgctggtacc tgaaaccagt gcgtctgcca gttccgccac
     7141 ttcggcctaa ggaatggagt gggattatcg ctgatcggtt tcgacccgtc aaggtgagga
     7201 acatgaaaat atccgtcggc tgaggtgaat cgagttcagc cggcggtcag gaccccgtcg
     7261 gtgagattcc aggcgccacc ggccgtcacc tcgcgcaggt cgccgctgag cacaatccgt
     7321 tcgcggacca accccgccgc cgccagcaga aaggccgcgc ggatgcgtgg cgcctcatcc
     7381 gcaatcaaga cgtcgaaccg caccttgcga aacaacggat cggccgccac ccgcgccgcc
     7441 gtcgtgacga tgacccgccg gttctgcacg aaggcctgtc gattcgtatc ctcctgcgcc
     7501 gccaacagct cgcgaatctt cttcgtgccc cccaaccgga tgacttcttc tttgagctcg
     7561 gcaaattccg gcttgagctc cttcgggacc gtcgcctcgg gaatcaattc ggcaatccgt
     7621 tccttggcga tgtcgagttc atggcgaagc ccggcacagt gccgttcata gatcgctttg
     7681 tactcacgca gcgattcgac gttttttcct accgcctgca tggacaaccg cttccagatg
     7741 gggagttgtt catagtcggt cagggtctgg tcgatttcct tgatcttggc caggaaatcg
     7801 gtcaactggg tcacgagtcg ccattccagc aaccgcacct catccagatc cttctgcttt
     7861 tgagccttgt aggccagaat cggcgtgagt tcgcgaaacc ggtcgtattt tttgcgcaag
     7921 accgccttgt cggatcgaga cttggcatag aactgatgca tctgcgcctc gaatcccaaa
     7981 tccggaagag gaatgcctcc gctctccttg ctggtcgcca cttcgtaccg actcagccaa
     8041 cttttgaacg tcagtccggc tgctttcatc gcccgggccg tgagaagggt gatcgcatcg
     8101 gcgctctcgt ggtccggagc aacgagaaga atgcgtttgt tgccccgaag cagctcaata
     8161 atgagcgtgg ccagcttctg ccgacgcgcc gtcagatcgt cttgccacaa gggcgtcagc
     8221 acggaggtgg gcaccagggc ctccagcgcg gcgcggtccc ccgtttgtcc cgcttcgagg
     8281 acggggagaa gccgttcggc cgggccgagt gtgtaggagg cgccggtttt gctcatttct
     8341 gccaagcggc ggctggtggt cagggccaat ccactggcat ccggcaccag cgtggcgctg
     8401 ggtaccacat cgccgatggc atcgaacaat tgcagcacga ggcgctcccc gtcctgaaac
     8461 agcacaatgc cctccgtggg ttcagcctct tcgcccagga gcagagtcgc agacagatcg
     8521 gctcccacca ccacgtcggc ggagagatag aactcataca ggtgcaaatt cccgacggtc
     8581 tggagcagcc ggcctcgcgt gagcggaatc gattggtcga gcccctgctc cgtgccgcgc
     8641 gcggcatcac cgtccagcgt cgccgccagc tgatcgagga gatcgggcaa ggtgtctggt
     8701 attgcggtca tgttccgttc ttcccctgcg tgtcgtcgtg gcgcagcctg cgatgccggt
     8761 cgcacagccg tcagcgcatt gttcatagaa tcaggaacgt atcttaaaga gttccgaaag
     8821 atcctgtcca ccagcgacgc acgcacaaac aatccaccgc tgaaacgcga caatgaacgg
     8881 aaagagccgc actgaacgcc tgcgccgcca tttcgagccg aatgaagaaa accatcgcat
     8941 gcctccagat caattgactt ttgcacagcc cgccagtatg ctttttccct ttccaacaga
     9001 aggagttgca gtatgaaggt gattctccaa gaaacactcg aaggcgtggg cgacctcggc
     9061 gaccttttgg acgtctccaa cgggtttgcc cggaactatc tcttgccccg caagaaggcc
     9121 gttgaagcca atagccggaa catcaaagaa ttcgaacatg ccaagcgggc cgcggccgag
     9181 aaggccaaga aggaaaagca ggacatcgag gcgcacggca agaagatctc cgcggtctcg
     9241 ctgaccgttg ccgcccaggt cggaaaagac gacaagatgt ttggatccgt aacggccaag
     9301 gatatcgcgg aagggttggc tgagcaggga ttcaccgtgg atcgccgcaa gatccagctg
     9361 gcccagccca tcaaggaact cggcaccttc gccattgcca tcaagctgcc gcgcgaagtc
     9421 accgcgacca tcgcggtcca tgtggtgaag aagcaagacg agcaagaagc gcaagcagag
     9481 ccggaagaag ccgcaccaac cgcataaccc gttgcgggat catgcgtggc caccggatag
     9541 aaggctgcgc tcggaggaag catgcaggag ctgatcggtt cgttggacgc attgaaatca
     9601 accgaccctg aaacctacga ggctatcgtc gccgaggagc agcggcaacg ggacaagttg
     9661 ctgttgatcg cctcggaaaa tttcgccagc cccgccgtct tggccgccca gggcagcgtg
     9721 atgacgaata agtatgccga agggtatccc ggcaagcgct attacggcgg ttgccagcat
     9781 gtcgatacgg ttgaaagcct cgccatcgaa cgcgcgaaac agatcttcgg cgccgagcac
     9841 gtcaacgtgc agccgcattc gggatcgcaa gccaatatgg ccgcgtacct gtccgtgctc
     9901 aagccgggcg acacgattct gggattagac ctcgcccagg gcggacacct cacccacggc
     9961 agcaaggtga atttctccgg catcattttc cgtgcgtttt cgtatggcgt cgatcgccag
    10021 accgaaacca tcgattatgc ggcggttcag aagatcgccg agcaatgccg tccccgcatg
    10081 ttggtggtgg gcgccagcgc ctacgcccgc accctcgact tccccaagtt tcaggaaatc
    10141 gccaagtcgg tgggcgcgta cctgctggtc gatatcgcgc acattgccgg attgatcgcc
    10201 gccgggttgc atcccaatcc cgtcccctac gcggatttcg tcacgaccac gacgcataag
    10261 acactgcgtg gcccgcgcgg tggcgtcacc atgtgcaagg ccgaatacgc caaggccgtc
    10321 gataagatta tcttccccgg cctgcagggc ggccccctca tgcatgtgat tgccgccaaa
    10381 gccgtcgcat tcaaggaagc gctgtcgccg gcgttcaagc ggtaccagca gcaggtgctc
    10441 gccaacgcgc gcacgctggc gcagggactc gtcgaccgtg gctataaaat tgtgtcgggg
    10501 ggaaccgata cccacctgat gctggtcaac ctcaccaaca aaggcatcac ggggaaagaa
    10561 gccgacgccg ccctcgacgc cgccggcatc atcgtcaaca agaacgccgt cccctacgac
    10621 gaaaaaccgc cggccacggc cagcggcatc cggatcggca gtcccatcgt ctcgacgcgc
    10681 ggcatgcgcg aggcggagat gcgtgaaatc gtggccctga tcgatcgtgt gttgcagcat
    10741 ccccaggatc agcaggtgca ggccgaggtg cgggcgcaag caaaaacgtt gtgcaatcgt
    10801 tttcccatct ttcatgccta cgattcgtct cccgcttagg caggacgttc gccagtgaag
    10861 tgtcctttct gcgacgatgt cgaggacaaa gtcgtcgatt cgcggatggc caaagaaggc
    10921 gaggtcatcc ggcggcggcg cgaatgcctg tcgtgcaaac gccggtacac cacctacgaa
    10981 cgggtcgaag aaaccatgcc cgccgtcgta aaaaaggacg gacgacggga gccgtttgat
    11041 cggagcaaga tcgtgtccgg gctcaagaaa gcctgcgaaa aacgcccgat cagcaccgcg
    11101 accatcgaaa ccgtcaccga tcgcatcgaa aagcgcatcc aggatttagg tgaaaccgag
    11161 atcgtgagca ccgccgtcgg tgaggaagtc atgagagaac tgtcccaact cgatcaggtc
    11221 gcctatgttc gattcgcctc cgtgtatcgg gaatttaaag acatcgacca gttcatggac
    11281 gagatcaaag ccctggccca gcagcgccgg gagcgttaag ccctcccttc tcttcgctgt
    11341 ttcggatcgg tccgctcggt gtaggtctcg gtaggatggg caccagcaga ggagagacct
    11401 gcgcactccc gataggctct gaacccatgg caacgaccaa agtcacccgc aaccgacgaa
    11461 cagccaagcg accaagccga cgccgatcgc cgggggccac acacgtggtt atcatcggcg
    11521 cgggccgcgg cggcactgcc ctaatggaga ttttcgccaa cgatcccttg gtccgcatcg
    11581 tcggcgtggc cgacatcagc gatcaggcac ccggcctcgg cctcgccaaa cggctgcaca
    11641 tccgcgtaac gcgcaactat cgccaactgc tgaaaatggg accagtcgat ctggtcatcg
    11701 atgtgtcggg caaccccgag gtcggcgaat acctgcagga cattcgccgc atgggcgtct
    11761 cggtcatcgg gggggccagc gccaaattca tgtggcagtt gatcgaggcg cgcattcgcg
    11821 ccacggccga catcgaaaaa gccttgaata agtaccagtc gctgtaccgc ttgtacgtca
    11881 aagaaaccgg cgccgcggtg acggaggaac ggacgcgcat tgcctgcgaa attcacgacg
    11941 ggttggtgca gagcctcgcc ggcgtgaact tcaagctgga gctctctcaa gagctgattc
    12001 ggaagaatcc caaagccagt ctcgccacga tccgtgaatc caaagcccaa ttgaaactgg
    12061 ccattcagga ggccaggcag gtcatcttca acctgcgtcc gctccactac gacaaaatgg
    12121 agctcatccc ggcactgacg aactatctca agtcctacga aacccaatac catatcaaga
    12181 cggccttttc cgtgaccgga gacgaaacga tcttgttccc gcgcaccaag atcttcctgt
    12241 tccggatcgt gcaagaagcc ctgagcaacg tgcaaaagca cgccaaggcc ggccgggtgt
    12301 cggtgcagct cgatattcgg tttgatctgt tgcaagtgac catttccgac aacgggatcg
    12361 gattcgacat ggatgcggtc cttcgcgatc ccgaaaagtg ggatcacttc ggcattcgcg
    12421 gcatcctgga acgcgcacga ctggtgggcg gggaagctac gatcgactcg aagaaagggc
    12481 gcggcacgcg catcgtgctg cgaatcccac tagccgacaa ggagacgatt cgtcatggaa
    12541 aaaattaagg tactgatcgc tgatgatcac cgcgtggtgc gcgaaggcct ggcggccatc
    12601 ctcaagacca aagacgacat caacgtggtg ggcgaagcgc aagacggcgt ggaagccgtg
    12661 gagaaaacca aaactctcat gccggacgtc attctcatgg atgtcagcat gccccgcatg
    12721 ggcggcatcg aagccacccg tcaaatcaag cgggaattcc ctcacatggg catcgtggcg
    12781 ctgacgatgt atgaagagca gcaatacatc ttcgatctcg tgcgcgccgg cgccaccggg
    12841 tatctgttga aagactctga gtcctcacaa atcgtggcgg ccatccgggc gatctaccgg
    12901 ggcgagtcgc ttattcatcc gtcggtcgcc agtaagatcc tggccgagtt ctccctcatg
    12961 tcccagaaga agggcaagaa gccggcttgg gttgaacacg atttgaccga acgcgaaatc
    13021 acggtcctgc gcctcgtcgc agacgggaag accaacaaag aaatcgccaa caacttggat
    13081 ctcagcgaga agacggtgaa aaaccacgtc cgcaatatct ttcacaaact gcaggtgtat
    13141 gaccggaccc aggccgcgat tctggccatc cgcaaagggc tgatcgaatt ggaaccgaga
    13201 ccctaacgga ggccgcgcca ccaatgcaaa gcccaggcac ggatctcgat attgatgccg
    13261 gaatacgtgg ttcccgctaa acccagcctg atacaatcag ttaactcatt gaaaaccaaa
    13321 cacttccgtg cttcgcctct tccatcaaaa gatacaattg tgtgaatttc accacagtga
    13381 gcactttcaa ccattcaata taattcaata tatatcaacg tcttacgatt aatttaatgc
    13441 ttggcacaag cattgctcag atgagagtac acattacgga ggaggcaccg tgcggtttca
    13501 gcaaacgatc ggatcaccag tttcatgttc gggcgtcggg ctccactcag gccaaccggt
    13561 cacgctgaca ctccgcccag ctccccctaa taccggaatc gtgtttgtct atcgcaacgg
    13621 atcggaggaa accctcctcc cggctgcggt ctcaaataaa gtaccgaccg agctctgcac
    13681 ggccatcagc gtcaacggcc gtcaagtgaa gaccatcgaa cacctcctgt cggctttggt
    13741 cggcatggaa gtcgacaacg tgtacgcaga agtcgatgca ggggaagtcc ccgtgctgga
    13801 tggcagcgcg agccccttcg tgcgattgat tcgtgcggcc ggcgtgatcc cccagacccg
    13861 tcgccaatcg tatgtgaaaa tcacgcaacc cattgaggtt gttgatggag cacgccgagt
    13921 cagaatcgag ccgtcttcca ccccgaagat cacgtactcc attcactacg atcatccatt
    13981 gatccaaacc cagtcctaca cctatgcctg ctccgcctcg gcttttgagc aggatattgc
    14041 aacggctcgc acgtttggat ttttgcacga agtcgaagcc ctgtgggcca gaggcctcgg
    14101 aaaaggcggg acactggaca acaccgtgat tctgtccaaa gacggtgtcg tgaatgaatc
    14161 gggcctacgc ttccagaacg agttcgtccg ccacaaggtc ctggacttga tcggcgatat
    14221 cgcgcttctc ggcttcccgt tcatcggcca tatcgttgcg gaacgatcag gccatgccat
    14281 gcacaccaga ctcgtcgagc aaatcctggc tcagcgcgac aaatgggccc tcatcaccgg
    14341 ggaacatgca gtcgcggcat ccgaatcccg ctcttcgctc gggctccttc gccccgcacc
    14401 atccctcgcg atctaaccgc tgcttctaag caccccgcat acgcagatgc actcctatgc
    14461 ctatgcccca taaggccatg gctgctttta ggcataaaaa aacgccggag gtaagtggcc
    14521 ttacccccgg cgtgtaaatc ctggtgggac ttacttcttc ttcgctgcct tcttcgctgg
    14581 tttcttcgct gccttcttcg ttgccaagac tctcacctcc cttcacacat ttagagttga
    14641 tatgaacttc ttccgcccgc ttacactact gccgttaact cctgggtcat caccgcaaac
    14701 gtattcggcc cgactttctg gaatcgctta tcccgcttta atgatgtcgc tacagacgtc
    14761 aatggtgtct tgcccttgat ctgcaaaccg ccctccagga ggcgctgcaa gagctccttg
    14821 gcatgcatcg gccggctggc ttcgcggaga atctgatagg cagcctccgg gacgctcttt
    14881 ccgacatact tgcttttgcc gagcaggatt tcacgcgatt ggtccgtgac atcggtcaca
    14941 gggagaggtc gaataccttt ttcatcggta ataatctgac tggagaggct cgccagcttg
    15001 gccttgtcgg cttcaacgcg gtacagcgtt tccgccagct ccaggtattt cttgatcatc
    15061 gcgatttcgt cgtcgagccg acgccgcttt ttctcaagct cctggtagcg attccgatag
    15121 gcgctgatcc gctgctccaa gcccaccaga atgtcggtca attcttccac aagcaagtcc
    15181 tcaataaagc atacttgctt tatagcaagt gaatcatttt ctgtcaagtg attcaaaaca
    15241 actatttgat cgcattctac gcgtgtgcac gccgaatgca tacaatgcct aaccaagcct
    15301 tgccatacaa gaacaagcgt tttatgtatc gttatttatc aatatcttac gatgagttaa
    15361 cagggtcaga cgaagtcttt tggaacgctt ggacgtgagt cgtggtagga tgcggcttct
    15421 atgcagacat ctgatcgtcc ctctccagaa gatcgctcct catttcttgc gaccgcgatt
    15481 cgagcggccg aagcagccgg aactgtgctc ctggagcatg cccgctccgg atttcgcatc
    15541 gactataagg cggccatcaa tcttgtgacc gatgcggatc gaggcgcgga agaaagcatc
    15601 gtgcgcacca ttctctccgc ccacccgtcc catcggatcc ttgccgagga acgcggggag
    15661 gatggggcca cggactcccc atatcgatgg attatcgatc ctctggacgg caccaccaac
    15721 ttcgcccatg gattcccgtt ttactccgtc tcgatcggcc tggaatgtga cggggaatgt
    15781 attatgggtg tcgtgctaga cccggttcgc cgggagttat tcaccgctgt tctggggcaa
    15841 ggcgcgtacc tgaacggcga gcgccttcgc gtgtcgagga ttgagacgct tgagcactca
    15901 ctgctggtca ccggcttcgc gtacaacatc cgtgagacca ccaacaataa tctcgaccac
    15961 ttttcccgca tctcactccg cgcacaaggc gtgcgccgta ccggttcagc cgcattggat
    16021 ctcagctatg tggcggccgg ccgattcgac gggtattggg aagtgaagct gagtccatgg
    16081 gacatggccg ccggcgttgt gattctccgg gaggccgggg gagtcgtgtc aggcttcagc
    16141 agggaccgat tttcattgta cggacaggag ctggtcgcca ctaacgggca cattcacgag
    16201 cacttgcttc gcgccatcac tcagcacccc gatcctcact gatcagcatc gattccagcc
    16261 ggcgatcccg agcaattcat ctccgaccac ggaaacacca cgccgcaggc cgtcccatgt
    16321 acagcgcgat atttgatgcg gtatcatgct gctctcacct gtcagactga aggagcgcaa
    16381 gcaccatggc aagccaagtc cctccgcaga aaccagtcac cccagcggcg aatccgcagg
    16441 atgtctggga cgtcgaactc ttacatgtcc acgtgtcacg caaaggacaa ctcgtcaatg
    16501 ccgaatgggc gctgcaccct cagatcaagg cagacttgag tgcggaggaa tggaaagaac
    16561 tcggcgagct gatgagcaaa gtcaccacca tcgtcggaca ccgtttttct caggccctcg
    16621 gcgaggccga tcccgcccct ccgggtaacg cctaacgcat cggacactga aaatccgctg
    16681 atcgctcgcc gacgccatat cgtgtccgga acgaaccaca cctactcacc ctgcgaggtt
    16741 atccatggac tgttattacc attcgaagga tctcggcaaa ttcggagaca tggggaaggg
    16801 caacccggtg ctgtgggaaa agttcatgag ttattacagc gccgtcttcg cggaaggggc
    16861 gctgacggaa cgggagaaag cgctgattgc gctcggcgtg gcccatgccg tgcaatgccc
    16921 gtactgcatc gatgcctaca cacaggcctg cttggaaaag ggatcgaata ccgaagaaat
    16981 gaccgaagcc gtccatgtcg cctgcgcgat ccgcggcgga gcgtcactcg tacacggcgt
    17041 gcagatgcga aacgtcactg agaagctgtc gatgtagggg aggggagaag gacggaagag
    17101 acgcagaatc cgctcagtag taggggtgct cgggtatggc cagcgtgaaa tacttcccgc
    17161 gctcttcata tagaatgcga cgactcgtca agatgccaag cgcctcggtc agctcgtctt
    17221 ccgagcatgc catgtcacgc gttgcctcgc tcagcgccgc ccgaatctgc acccgactct
    17281 tcgcagcctc gttgcacgcc tcgatgatgc ccgccgcagg ccagtcgtac cgtcgtctcg
    17341 taggcatctg tggattacgc ccgtcataga tcgtgacata ctccatggcc ttggagtaat
    17401 acaaaaacgg ccggtcgctg ctgcgcgcca gccgctgcca ttcctccacc aagccgatca
    17461 attcctgata gacgtgggga tccaccggcc agtcgtcgag ttcatactcg aaatcgtagg
    17521 cgatcttgcg cagatcgacg cgcaccgcat catacacata ttcataggct gtccccggac
    17581 ccatgatgcg cacgccgtac tcatgcggtc tcgtaaagta gggactgaac cgttgcaacc
    17641 agaatttccc ggtcgcctcc ggcgcttgca aatggaatag ggacggcagg agatcgatct
    17701 gccgccgata gtcctcattc gtctcgccgg gaaagccgag cagaatattc caggataccg
    17761 acactcggta gtaacaactc cacttgaggc aaatgatgtt ctgcatgggc gtcacgccct
    17821 tgtccatcgc tttcaactgc gagagactga ggctttccaa gccgggctgc atgcacttca
    17881 cgccccccac ggccagcgtc cggatctggc tcttctgcag attgctcttg gtttcgataa
    17941 acacatccag atcgcagtgc tcggcggcaa accggccgaa cagattctcg acatatttca
    18001 tatcgatgat gttgtccacc agccggaacc gtgtcgtatc gtagcgactc gacagatacg
    18061 ccatctcgcg agcgacctgt tcagacgact tggcgcggaa tttcatactc tgcgcattca
    18121 gcccgcagaa ggtgcaatgg tgcttctcac cccaccaaca accgcgtgag ccttcgtaca
    18181 atagaatacg atccaggccg cgcgacgcct cggtgccgag ttcggccagc agatgataat
    18241 agtcgtcgta gtccggcggg ccggtctgtg cgaactccgt gaagagcgcg gtattcggct
    18301 cacatcgaat gtcgccctgc tcccgatacg tcaccccttt cgggcagggc ccggccgatc
    18361 cgctcaagat atgggtcacc aatgcgggga acgtcacctc accttcaccc acgaccacat
    18421 ggtcgatgaa cggaaaggcg cgaaagtatt ccaaccccat ctcgccgtcg tagttggcgc
    18481 caccgaacac gatcttcacg tccggataca aatccttgat gagcttcgcc atggtcagac
    18541 tggccacgtt ttgatcgaag gtggacgtga accccacgat cttgtactgg ccccaatcga
    18601 tgccggtcat ggctccggtc aggaattgcg gcgcggtccg cgtggccatc tcctcgaaga
    18661 acgaaatcgg ctggccgctc tcccgggcaa tctgctcgaa caccggcttg aacaccctcg
    18721 gatactcggc ccgttggggg ttgtcgcgaa acagcagata ggagaagagc cactcgccga
    18781 acagcgcgcg tttctcgcaa atcgattcgt agagaggaat cccgatcttg tgcgcgaatc
    18841 gcacattgag gtgatgacac tcgaccccga tgcccttcgc tttcaacagc gccgacaacg
    18901 tgcccaattg aatggaagga tatttcgaaa aactgaaggg catgttgacg agcgccacct
    18961 gggcctggga gctactcata agatccccag tccagccggc acaaggcggg ggccaaaccc
    19021 ggagaagtca tggacatcgt cacgagacag tcgagaccga agctcgctgc gggcgcccgc
    19081 agagaggacg acgcaggcgt attcaccgca atccagcgag gaggccgagc gagaacgtcc
    19141 gcgccgagcg agaggatcgg ctgtcgcagt agatgcgcca tgacttctcc tggttaggcc
    19201 cccgcccgaa acggttcaaa ctctcgatcc gctttggcgg ccagataaaa atcgctctcc
    19261 gtgaggccgt tgatcttgtg tgtccagatc tcgactttac attttcccca ggctacatac
    19321 agatccgggt gatggccctc ggcttcagcc actgcgccga ccttattcgc aaatgccagc
    19381 gactgcgcaa aatccttgaa tgtataaagc cgttcgagat ggccttgggc attcagcgac
    19441 cacccacgcc ccacctcttg caacaaggtc tgtgtacgat cggcggggag gggagggact
    19501 ccgcctcgac acggaataca tttattgtcg gccagactca tgacatctcc ttcgcagacg
    19561 gtgacagccg tcggtcgttt caacaagggg ctattctacg agttgcccgt tcggtcccgc
    19621 aaccgcccta tcgccctgac ggttgtgcga ccctgtgttc atccatcacg gcatgccctc
    19681 gttcgaaaaa gagccctcga ttgatgtcgc ccacgctgac catcccgacc aatgtcccct
    19741 tctcgacgac cggaatccgg cgaaaatttt tcaagcccat ataggacgcg gcttcaagca
    19801 ccggggtccg tggcgtcact gtcagcggat tcaggctcat gacctcctca actcgttgag
    19861 tgaggacgtc cgcatagccc tcctccatct ccacaaaatc ccggctgtgc acattgtcat
    19921 gaatatattc gccgtaattg ggataaagag gaatcaagac atcgcgcagc gtcaccatgc
    19981 cgatcaagag ctcaccgtcc tcgacgaccg gaatggcgcc gcaatgacga ctcagcatct
    20041 tggtgaccac gctacggacc gactcatttc gcctggccgt gaccaccccg gtagacatga
    20101 catcctggac aagcatggca gcctcctttc acacaaggcg actcgcagag gagtcaccgg
    20161 aaggggaatg tattctgcat catcactcgc tttgatccaa gccacctccg gccacatcat
    20221 ccgatcctgg cagagccggg accgggtccg ccgctaacca ttgtatcaac tcaccggagg
    20281 gtgggcccct cggcgttctt tgcagactta cgcgaa
//
LOCUS       DAORRQ010000071        18921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_168_length_18921_cov_86.530955, whole genome shotgun sequence.
ACCESSION   DAORRQ010000071 DAORRQ010000000
VERSION     DAORRQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18921
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168_length_18921_cov_86.530955"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK224_17120"
     CDS             complement(<1..662)
                     /locus_tag="PK224_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17520.1"
                     /translation="MTVRLFQDRTGCRQWRITGTWLALSLFCAGCAIEQEISRTPRTA
                     VEQLLLTGAVDQALTNLTLTLPAQADLHLEVSGFYIDRGRLNMRDQTSGTVQDPTVDF
                     LFVRDTVAVALGRMGYRIRKRDDHPAYLVRVAIEALGTMQGLTFVGMPPIQSVVVPFA
                     LPELTLYKLQAQSGYARFHLDVFDNLTGAYKGSSATIIGRTHYDQYTALFYITWIETD
                     LTA"
     gene            complement(730..1470)
                     /locus_tag="PK224_17125"
     CDS             complement(730..1470)
                     /locus_tag="PK224_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRB17521.1"
                     /translation="MRRRPSLVLSFLPFLLGFAFLSIWPASVSAEQIKATIPAPQTAS
                     WWSARHARAVARIQQGPVDLLFIGDSITQGWEEDGRRVWDTFYGRRQAVNLGFNSDQT
                     DNVLWRLQHGEIDGITPKLAIVMIGTNNATRREDPPEETAAGVHAILTTLRTRLPQTK
                     ILLLAVFPRGQSANDQLRRVNQAVNERLRAFADQRHVFFLDLGPRFLDKAGRLSEEVM
                     PDALHPNERGYRLWAEGMEDLVKTLLGE"
     gene            complement(1616..2296)
                     /locus_tag="PK224_17130"
     CDS             complement(1616..2296)
                     /locus_tag="PK224_17130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRB17522.1"
                     /translation="MPNPSTYQFNLPQRGRPSDVMLNEVWYPGIQAYWETSTSSRIFD
                     AILGIKALVIHATAGSSSAGAVSVMKEGKASFHWLLPDEDEPQHGQLVWACAPEARAA
                     WHVRNSCSHPSINDGADKVNHWSLGIEVVNRQAGSDGFSDWQIQATAQIVRYCWAKYP
                     NLKHVVSHAKLDPARRSDPGTAFPWARLKTLVLTPEAEDGVPSVALDAQPASSIKSTT
                     GHEGCCHG"
     gene            complement(2497..3087)
                     /locus_tag="PK224_17135"
     CDS             complement(2497..3087)
                     /locus_tag="PK224_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17523.1"
                     /translation="MAPVTVNALLTPGFPVAVLFGGNHLPSHATGRTAAIRQPIGGTQ
                     DTPEPMNRRWFRYKTTAVAEYRNGLTFVGHWAAEKHLTLDFLFPVAHGGALAGSLWWV
                     WATLGHPFHPAWIAAPLAMILTADWTEHLIQLVQLRHYLPSTEGRIQSLWVRISNCAT
                     IIKLWLTFGFYVSLAGLVVRMILTLSDRRLVTDAGE"
     gene            complement(3343..3699)
                     /locus_tag="PK224_17140"
     CDS             complement(3343..3699)
                     /locus_tag="PK224_17140"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRB17524.1"
                     /translation="MTRRAEKNRWELTHWGDRSVPYGDDGEPVILSFKDGRVSSHAGC
                     NRYSAAIVFGPHRGELKVSNGITTRMACEPRHMEFEASFIRAFEASTRYRLDGESLSF
                     ESDIAPPLEFYRRPLD"
     gene            complement(3888..4262)
                     /locus_tag="PK224_17145"
     CDS             complement(3888..4262)
                     /locus_tag="PK224_17145"
                     /inference="COORDINATES: protein motif:HMM:NF040323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HRB17525.1"
                     /translation="MQLGTPIPILRIFDEAKAKEFYLDFLGFRLDWEHRFEAHLPLYM
                     QISKDRCILHLSEHHGDCSPGAAMRIETTGLDAFQRQLAAKQYRYARPTIDLTPWDSH
                     DMSVTDPFGNRLTFTDTRGTQS"
     gene            complement(4398..4940)
                     /locus_tag="PK224_17150"
     CDS             complement(4398..4940)
                     /locus_tag="PK224_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB17526.1"
                     /translation="MLSSNKLPTPRLLLRQWRPSDLEPFAAMNSDADVMRYYPTPWTR
                     EQSDAFAQRVMRLIDERGWGFWAVEERASGRFIGFVGRHVPSDELPFSPCVEVGWRLT
                     KPYWGLGYATEAAQSAISFGFQQLRLAELVAFTAIANLKSRAVMERLGMRLDCEFDHP
                     QLPVESWLRRHVLYRLRSPV"
     gene            complement(5264..6013)
                     /locus_tag="PK224_17155"
     CDS             complement(5264..6013)
                     /locus_tag="PK224_17155"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRB17527.1"
                     /translation="MGKCYGGNDFVVGVLVEAHGMLLDLLIIGFVVVWLNRRAQQAHE
                     SVQYQEEIDSLRGWKDREAGVQILHNIRRLNRNGITSIFLSGAVFDGLDLRGDGEPWA
                     TKSNVDLSGAYAPGASFIESILTGISLSETNLQQCNFTGACCGSVDFSGALLLGADFT
                     NAHLARANFTNAHHLKASQLAKARNLHAAALSDRLKVEVLELNPAIFDPEPFKSHIAD
                     FFELLDHQELAEESVRARRGARTSHGRASDT"
     gene            complement(6349..7026)
                     /locus_tag="PK224_17160"
     CDS             complement(6349..7026)
                     /locus_tag="PK224_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17528.1"
                     /translation="MSPRNRKLLAATAGLIFVLAGVAVALVGQFVVAAIGDILLATNS
                     IPGWSVPQDRSSFYTLFVLGLCVAAGLSVTALFWSPRPGYSIRLTIYVLLLVALLPIS
                     VFNYAHADYLVNRSAQALFNVLLVFGGIVVVAELLLFRSSGRDLIVVQVLAVFTLSLT
                     AVFIPATFSVVWLLNALGVVTATAAESISLPTLSTVSGVFSAAVAYLKFRREIKTEEK
                     SSLIVSG"
     gene            complement(7428..8561)
                     /locus_tag="PK224_17165"
     CDS             complement(7428..8561)
                     /locus_tag="PK224_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HRB17529.1"
                     /translation="MRRQLERWQSSYTMKTCPFCKEQINDEAIKCRFCHSMLLPVQVS
                     EQKPLDDGRVTYVLDRDLIRFGKFSGAVLALFLVVGAYLFGFKLESALEKVRDTQEQL
                     KTTQEKLSAAQRDLEAAQATVKVLKRDVEAVLAEAKSSLGEISEQKAAAIAMVVSIRE
                     LTPRETAALQKARAEQPDKIRRGAWSKFWANGTIIRILFLDGDAKSHEAVKQVAQEWA
                     KYANLRFQFVTSGDSEIRVSFKQEGSWSFLGTDALAVAKDQATMNFQWIDRRTLLHEF
                     GHAIGLIEEHLNPKARIHWNRDLVYRELSGPPNFWSKEQIERQVFQRVSEAQLGEYRD
                     FDPGSVMTMTFSPTWTGGTILGKRQDLSESDKALVARIYPRGN"
     gene            complement(9034..9663)
                     /locus_tag="PK224_17170"
     CDS             complement(9034..9663)
                     /locus_tag="PK224_17170"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRB17530.1"
                     /translation="MLHTIILDSRTVSRLVLLLLVTSPLTPALSAMEESRSTLHTYTP
                     AATNDPGPFHCNDCWGLKPEDRQPLPQHQAPRYRRGQHERGPRPHKNPFAKKSRFHSR
                     GLRSLPRHALSMGGFERTVEPWQVRTVDGDTIRYGTDRIRIRGYNAPELSEPGGREAA
                     LRLEQLMQEGIINIVPHGRDVYGRTLADVFVNGQNVAEVMTGEGFGRRG"
     gene            complement(9745..10581)
                     /locus_tag="PK224_17175"
     CDS             complement(9745..10581)
                     /locus_tag="PK224_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17531.1"
                     /translation="MQPEIVQAVGERWVWILDIVFGSVIGFGFQKLEEGLRLTARSSV
                     SAFMKHLFMAAGFLGFVIYDIGVYHILIKNFPYDVSSLSAARYVLDLIMAFLLMVILV
                     RGLSIDAGKHVFEILIALSLWHIAAMCWHFAASMQRNGVMPDVNTFSPHVAFIAMYWL
                     TFALWYFPNGEDRQSKLAEPTGFLVFLAAALLLISIWRSHQMIQKFVSEPTHDHTRQA
                     SLSMTPAGPGQPDDSLPSISSLPQGPAHCGKHAAVPGMNLHRLLLDLDTGLKGEFLAY
                     SR"
     gene            10818..11051
                     /locus_tag="PK224_17180"
     CDS             10818..11051
                     /locus_tag="PK224_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17532.1"
                     /translation="MDMFGKVGLVEVPILIAPDQKAWMDRMIKEGKIAIPPGGTLEKG
                     SLYSMFVRMLLHNAMEEQKRQEALEMDDEDDDE"
     gene            complement(11317..12600)
                     /gene="hemL"
                     /locus_tag="PK224_17185"
     CDS             complement(11317..12600)
                     /gene="hemL"
                     /locus_tag="PK224_17185"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRB17533.1"
                     /translation="MKTQRSEQLFADAQLIIPGGVNSPVRAFRSVGGQPRFIARAKGS
                     RLYDVDGNSYIDYVLSWGPMILGHAPATVTKAIQQAAGNGTSYGAPTELEIQLATMIR
                     EALPSMEMVRLVSSGTEAVMSAVRVARAYTKRDGILKFEGCYHGHSDYLLAKAGSGLT
                     TLGIPDCPGVPEDFTKHTLTAPYNDLATTEKLIKRHYRQLACVIVEPIAGNMGVIPPS
                     PEFLHALRKLTDAHGMLLIFDEVISGFRVQYGGAQTLYDIKPDLTILGKIIGGGLPVG
                     AYGGAKDIMKMIAPSGPVYQAGTLSGNPLAVTAGIETLKRLKKPGTYEQLEQRSVALS
                     EGVGNAARKAGVPLTQTRVSSMMCAFFTQGPVTDWASAKKADTKAYAKFFHQMLDAGI
                     YLAPSQFEAAFMSVAHTPKDIDRTISAAQAAFKNL"
     gene            12866..13096
                     /locus_tag="PK224_17190"
     CDS             12866..13096
                     /locus_tag="PK224_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17534.1"
                     /translation="MKVICSWCQSEGRPGLVREKAPLADLRETHGICSDHLQQMAVGR
                     DPLIPGYDEGSLPRHESVVIRRSDLAAVACYE"
     gene            13281..13580
                     /locus_tag="PK224_17195"
     CDS             13281..13580
                     /locus_tag="PK224_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17535.1"
                     /translation="MRLLRPQRGWSHRNERSEVVKVLCAWCIRDGKPAFLREKFPLED
                     PSETHGLCGDHFTSLSASVGKIVTPRVWMLSRMHDLSWGLTRWAQRMMGRFFSLC"
     gene            complement(13629..14036)
                     /locus_tag="PK224_17200"
     CDS             complement(13629..14036)
                     /locus_tag="PK224_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HRB17536.1"
                     /translation="MGLLDQLGQAAAGMMGGGDKNPLMQAVVNLLGQNSSVGGLSGLV
                     QAFQKNGLGDIVNSWVSTGKNLPVSAEQIQQGLGGDLLKQLASQAGLSSEAAGSQLAN
                     LLPGLIDKLTPDGKMPDTTLIEQGLNLLRGKLG"
     gene            complement(14073..14609)
                     /locus_tag="PK224_17205"
     CDS             complement(14073..14609)
                     /locus_tag="PK224_17205"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRB17537.1"
                     /translation="MSLIALQSIKKPWAHLLVHAEGEALDKILSVPAHFVTKTIAGKK
                     CKTKAGLLDEFSRVFSFPDYFGHNWDALEECLADLDWLPAKGYLVVVTDADQVLTKPD
                     EEDDFETFVEILSEAGEAWSLKESDEATGDGLPFHAVLAVSDRHKRSRHNWFAPPLAM
                     ERKPSKSTPAKGAKKPTR"
     gene            complement(14616..15080)
                     /locus_tag="PK224_17210"
     CDS             complement(14616..15080)
                     /locus_tag="PK224_17210"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HRB17538.1"
                     /translation="MMSIRRITRLLLWLCATVALSLGVSFATEPGQTLLDAPPPILTP
                     APDQTPQTQNGSVRAGPQATTRSDQASIPLIARETLSAIETRHGDPPPGYIGGRTFQN
                     RERVLPRGRYREYDVHPKVPGKNRGAERIVIDQRTGKAYYTADHYRTFVPMN"
     gene            complement(15357..17696)
                     /locus_tag="PK224_17215"
     CDS             complement(15357..17696)
                     /locus_tag="PK224_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRB17539.1"
                     /translation="MSASDSQSTDQLDFVTMQGLLAYGESLAQRTSSGRFVLPPPPLP
                     AADRLPRAEEAIGRIRIFIQQAQAGLSSAEAYRTARQAVITDACGSDPLVFFAAWNLL
                     LARGELAPLYRAPIGATQKPAHRRPVAIVPRTQLTPQLAEGRIVLDLGDDRFWLLPRD
                     LGDRTLFLTMRHGVSEVESKTHRVGRRLANVLDAERGIPRAEAVGAALARMVGVVGQQ
                     LGYLQLRNYLDPRTFLHLISHSPNTEQLCRRITKALIPDRADAVHPHVEATLESQDFG
                     WVTGMEKQAEIDAAAQAFGVDAKTAKRLIKHPFYSYPGGHSFFDLYIDVIDGLHRLGR
                     AHPGEVLCLYTHSSTLRALRIYLDPRPFREAFSEFGEYKEGQDNVVLLTLENGKLSGY
                     STAVGLIESERVAREAWVTVERERSQRVTLKPQRIKRLLALVSGGDFGGGGAALKELR
                     VTGNRFGLEVFFVRHGFLGLANNWIDPVTEEDTRGMGSHASSPIGSSRFEDFKDEEVQ
                     RAAMRSLAPYLADSALVVLGGDGSLRGARAIHETYGVQVVGIPGTIDNNIAGTTSLGF
                     HSAIALANQSIESLKATSAAMGSIFFVEVMGAGSGHLALACAYQARAEGILVNEHPDP
                     DAYIEEIVLGTLKRTLGVPNKSHIFVVAERTPHRHHREGGVHGLVDYVANTIATWPQR
                     REKSGHYALATATKATILGHTLRGAPPTPEDKMIAQHLAYETVRRLVEQPDTIVGCMV
                     AYHDSNRLETIPLHAVAPKPFDWELFTRMHGTELAPDRV"
     gene            17905..>18921
                     /gene="pyk"
                     /locus_tag="PK224_17220"
     CDS             17905..>18921
                     /gene="pyk"
                     /locus_tag="PK224_17220"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRB17540.1"
                     /translation="MRKAKIVCTIGPASDGAVVLDQLIRSGMDAARLNFSHGTHDSHG
                     RAIKTIRDTADRHGVAIAIIQDLQGPRIRVGEIDGALELIAGQRVRLRTMSLRSGGQI
                     GARTVIAAGTAQDIPVTYQALTRDIRPGAKILIDDGLVELTADRIVDGAVECTVMAGG
                     RVTSHKGMNLPGTVVSAPTLTEKDREDVRFGVAQGVDYIALSFVRGAQDIMAAKQLIA
                     ECGGDVPVIAKIERQEAVTDLEAILVHADGVMVARGDLGVELGPEAVPVLQKRIIATA
                     NRQRRLVITATQMLESMTQHLRPTRAEASDVANAVFDGTDAVMLSAETAVGHYPVEVV
                     QVMDR"
BASE COUNT         3918 a         5318 c         5714 g         3971 t
ORIGIN      
        1 gcggtgagat cggtctcgat ccaggtgatg taaaacaggg ccgtgtactg atcgtagtgt
       61 gtacgaccga tgatcgtggc ggacgatcct ttgtatgcgc cggtgagatt gtcgaagaca
      121 tcgaggtgga atctggcata gccgctttgg gcctgaagct tatacagcgt cagctcagga
      181 agggcaaagg gaacgaccac gctttggatg ggcggcatgc cgacaaacgt caggccctgc
      241 atggtgccga gcgcttcgat ggccacacgg acgaggtagg caggatgatc gtcgcgtttg
      301 cgaatacgat agcccatccg gccgagcgcc accgcgaccg tatctcggac gaacaggaaa
      361 tccacggtgg gatcctgcac cgtgccggag gtttggtcac gcatgtttaa tcgcccgcga
      421 tcgatgtaaa acccgctgac ttccagatgc agatcggcct gcgccggaag cgtcagtgtg
      481 agattcgtca aggcttgatc cacggctcct gtcagcagca gttgctccac cgccgtccgc
      541 ggcgtacgcg aaatctcctg ctcgatggca cagccggcac agaacagtga gagggcaagc
      601 cacgtgcctg tgattcgcca ttggcggcaa ccagtcctgt cttggaataa tcgaacggtc
      661 atgtcatgaa tcctagcacg ccggtgctgt tccagccgga tgagatagtg cccctgcacc
      721 gacctgtttt cattcgccca gcaatgtttt aaccaggtct tccatgccct cggcccatag
      781 gcggtacccg cgttcattgg ggtgcaaggc gtccggcatg acctcctcgg agaggcgtcc
      841 tgccttgtcg aggaaacgcg ggcccaggtc gaggaaaaac acgtggcgct ggtcggcgaa
      901 ggcccgcaat cgttcgttga cggcctggtt cacgcgacgc agttggtcgt tggcggactg
      961 gcctctggga aacaccgcca ataacaagat tttggtttgc ggcaagcgag tgcgtaacgt
     1021 agtcagaatc gcatggactc cggcagcggt ctcttccggg ggatcctcgc gcctggtcgc
     1081 gttattcgtt ccgatcatca cgatcgccag tttgggagtg atgccgtcga tttcaccatg
     1141 ttggagccgc caaaggacgt tgtcggtctg atcgctgtta aatcccaggt tcaccgcctg
     1201 acggcgtccg taaaaggtat cccagacccg gcggccatcc tcttcccatc cctgcgtgat
     1261 ggaatctccg atgaacaaca gatcgacagg tccttgctga atgcgggcca cggcgcgcgc
     1321 atgacgcgca gaccaccagg atgcggtctg tggggcagga atcgtggcct tgatctgttc
     1381 tgcggagact gaggcgggcc agatggagag gaaggcaaac ccaagcaaga aggggagaaa
     1441 agacaggaca agggatgggc gacggcgcat acggaccctc cagcgatgat ctgtgattct
     1501 tatcgcacga attgcgtcag atcaagcctg tgcgctcgcg ccgagggccg gtggtgcaca
     1561 cacatctcac gcgaggtcat cggttaggtc aaggcctcac tgcaggctct ctgcgtcatc
     1621 cgtggcaaca gccctcgtgg ccggttgtcg attttatgga ggaggccggt tgcgcgtcca
     1681 acgcgacgga cggaacccca tcttccgcct ccggcgtgag gacgagtgtc ttcaggcgcg
     1741 cccagggaaa agccgtgccc ggatcgctac gccgggccgg gtccagcttc gcgtgcgaga
     1801 cgacatgttt caggttggga tatttggccc agcaatagcg cacgatctgc gcggtggctt
     1861 ggatttgcca gtcggaaaat ccatcgctgc ctgcctgacg gttgaccacc tcgatcccga
     1921 gcgaccagtg gttgaccttg tcggcgccgt cgttgatgct cgggtgtgaa caggaattcc
     1981 tcacgtgcca ggcagccctg gcctccgggg cacaggccca gaccaattgc ccatgctgcg
     2041 gctcatcttc atccggcagc agccagtgaa acgacgcctt gccttccttc atcacggaga
     2101 cggctccggc cgaactcgaa ccggcggtcg catgaatgac cagcgctttg atgcctaaga
     2161 tggcgtcgaa gatgcggctc gacgtgctgg tttcccaata ggcttgaatg ccggggtacc
     2221 agacttcgtt gagcatcaca tcggatgggc gccctcgttg cggcaggttg aattgatagg
     2281 tggagggatt gggcatggtg gttcctccgt tggactcctc ccgagtctca cacctctgag
     2341 actgaaccct tgcgcagcct cagcacgagg tggtggcgag agaaagcaaa tgggactgat
     2401 cctccgttgc cgtggatctc ttcacgacac ggctcggcaa tgcgtatagc ataaacggag
     2461 agaactgtaa ggagtccgac aacgttttgt tcctcatcat tcgccggcgt cggtcaccaa
     2521 acgacgatct gagagcgtga gtatcattct gacgacgagt ccggccaggc tgacgtagaa
     2581 gccgaaggtg agccacagct tgatgatcgt cgcacagttg gaaatccgga cccacaggct
     2641 ctgaatacga ccctcagtcg aggggaggta atggcggagc tgcaccagtt gaatgaggtg
     2701 ttccgtccag tcggccgtca gtatcatcgc aagcggcgct gcgatccatg cggggtggaa
     2761 cgggtgcccc aacgtcgccc agacccacca gagactgcct gcgagtgctc ctccatgcgc
     2821 gaccggaaac aggaagtcga gggtcaggtg cttctccgct gcccagtggc ctacgaacgt
     2881 gagcccgttc cggtattccg caacggcggt tgttttatat cggaaccagc gccggttcat
     2941 cggctcgggt gtgtcttgcg tcccgccgat cggctgacgg atcgcggcag tgcgcccggt
     3001 cgcatgagac gggagatggt tgccgccgaa cagaactgcg acggggaagc caggagtgag
     3061 gagagcgttg actgtcacag gtgccatgat gtgaagctcc agtctctact ccattaatga
     3121 gcaaaaccga tgccggggtg tgatgagaaa gaggcacatt gagggatacc gattctgagc
     3181 ggggtgagcg aagccgcaaa gtggaaaggg ctgtgtggag gccgcagatc ggtatcagaa
     3241 gcgataccct gtcgtatcga aagggatacg gtatcgaaga gaagaggtgg gggagtgaat
     3301 cgattcgggt ccggatcggc aagagaggtc gaaagtttct gatcagtcca gggggcggcg
     3361 gtaaaactcc aacggcggag cgatgtcgct ttcgaacgac aagctttcgc cgtccaggcg
     3421 gtaccgggtc gaggcttcaa atgccctgat aaacgaggct tcaaactcca tgtgccgggg
     3481 ctcgcaggcc atgcgggtgg tgatgccgtt ggagaccttc agctcgcccc tgtggggtcc
     3541 gaagacaatg gccgcactat accgattgca ccctgcatgg ctcgacactc gtccatcctt
     3601 gaacgagaga attacgggct ctccatcgtc gccgtaggga accgaacgat caccccaatg
     3661 cgtcagttcc catcggttct tttccgcgcg tcgagtcatg tcctccggtg aaggcgacga
     3721 gacgcaggat gccagtagca gtgcggcaca cattgcgagc gaaatatgaa agaagtgtgt
     3781 tggcatgcgg cggtcctcca gtcaaaaatt cctgaatgat gagttcgtcg ggagatgaga
     3841 tgacggccgt tcgtgatccg cgtcgatgat gctcgtacag cagcggccta ggactgagtg
     3901 ccgcgggtat cagtgaacgt caatcggttt ccaaaggggt cggtcaccga catgtcatga
     3961 ctgtcccatg gggtcaggtc gatggtcggc cgggcgtatc gatactgctt cgccgcaagt
     4021 tgccgctgga aggcgtccag accggtcgtt tcgatccgca tggccgcccc gggactgcag
     4081 tcgccatgat gctcggacag atgcagaatg cagcgatctt tcgagatctg catgtagagc
     4141 ggcaagtgcg cttcaaagcg atgctcccaa tccagtctga agcccaagaa atcgagatag
     4201 aattccttcg ccttcgcttc gtcgaagatg cggaggatgg ggataggtgt tcccagttgc
     4261 atcgaagcgg attgtacgat gagctcgaat cgtttgtcat gacgaagggc agataatccg
     4321 acaatcgatc gttattgagt gagggtcgtc gcaagacgga tgagcggtga tactcgaaac
     4381 gaaggaggag ggtcttctca cacaggcgag cgaagacgat acagcacatg tcggcgcagc
     4441 caggattcga cgggcagctg aggatggtcg aactcgcagt cgagccgcat gccgagacgt
     4501 tccatcaccg cacgcgactt caggttggcg atggcggtga aggccacgag ttctgccagg
     4561 cggagttgct gaaagccgaa cgagatggct gattgtgcag cctccgtggc atagcccagg
     4621 ccccaatagg gctttgtcag acgccagccg acttccacgc agggagagaa cgggagctcg
     4681 tcggagggaa catgtcgtcc gacgaaaccg atgaatcgcc ctgacgcccg ttcctcaacc
     4741 gcccagaagc cccacccgcg ttcgtcgatc agtcgcatca cccgttgggc aaacgcgtcg
     4801 ctctgctctc tggtccaggg ggtcggataa taacgcatga catcggcgtc ggaattcatc
     4861 gccgcgaatg gttccaggtc tgatggtcgc cactgacgga gtagaaggcg gggagtggga
     4921 agtttattgg aggagagcat ggcagcgttc acgtttaagt taggccatat gagggcagtc
     4981 taggcaaagt gaacgagcct tgggagtcgc tactggaaag ccatgcttga ttgcaagacc
     5041 tgctcctaat tggtagaccc tatttgttct gggaccaccg ttttgcgggt ccggcgcaca
     5101 caggtttgaa tcctggaccg gcagcttcgt cgttcacacg atcaggttgt cagaatcgct
     5161 cgccaacaat gactctcgat ccctttcttc accccatgcc taacgtttga gttcacccgc
     5221 gtgcggaggc cgcaggccgt agcacgtctg gtggaacgaa gggttaggtg tcactcgcgc
     5281 gcccatggga agtacgagct cctctgcgcg cgcgaacgga ctcttctgca agctcttggt
     5341 ggtccagcag ttcgaaaaaa tcagcgatgt gggatttgaa tggctccggg tcgaagatgg
     5401 ccggattaag ctcgagcact tctactttca ggcggtcact taaggcggcg gcgtgcagat
     5461 tgcgagcctt tgctaactgt gatgccttga ggtggtgagc gttggtgaag ttggctctcg
     5521 ccaagtgcgc attcgtgaag tctgccccaa gcaacaatgc gccactgaag tcaacgcttc
     5581 cgcagcacgc accggtaaag ttgcattgct gtagatttgt ttcggaaagg gaaatgccgg
     5641 ttaagattga ttcaatgaaa cttgcgcccg gtgcgtaggc tccgctcagg tcaacgttcg
     5701 acttggttgc ccatggttct ccatcacctc gaaggtctag gccatcaaag acggcgccgc
     5761 ttaggaagat ggaagtaatc ccattgcgat tcaggcgacg tatgttatgg aggatctgga
     5821 ctcccgcctc tcgatccttc cagccacgta acgagtcaat ctcttcctga tattgcaccg
     5881 attcgtgagc ttgttgggca cggcggttta gccagacaac gacgaagcct attatgagca
     5941 gatcaagaag cattccatgc gcctctacca agacgccgac aacaaagtcg ttgccgccat
     6001 agcatttgcc catgtagcac ggcctgctta tggtgaccag aaaaaatgag agtaccgcga
     6061 acaccactag cgcgattctc agttctgtcc caaggaatac agcagcaagc ggtcttcggt
     6121 agttatgcgt tgtatgtggg tttggcctgt cctcaagttt tccgtgtccg tatggatttc
     6181 gcatgatgca gtctcacgat aagttcgtat tgctcaggtg agccgtttgc aattagactt
     6241 tccgctgagg atgtcgccca ttgtgacgcc taacgttgga attcagcgga cggcaaaaag
     6301 cgcagctttt tgacggttcg ctggaatgaa tggttgtgcg tcatgttgct aacccgaaac
     6361 aatcagggat gacttttctt cggtctttat ttcacgccga aactttagat aagcaaccgc
     6421 cgcagagaag acccctgata ccgtggagag cgttggaaga cttatggatt ccgctgctgt
     6481 tgcagtgaca acgcccaacg cgttgaggag ccacacaaca gaaaatgtag cgggaataaa
     6541 tacggcggta agggataacg tgaatacggc gagaacctgc acgacgataa ggtcacgacc
     6601 ggaagaccgg aagaggagca gctcggcaac cacaactatg ccgccgaaca cgagaagaac
     6661 gttgaacagt gcttgcgccg agcgatttac tagatagtcg gcgtgagcat agttaaaaac
     6721 ggagatgggc aataaagcta caagcagtag gacgtaaatc gtgaggcgta tggagtagcc
     6781 cggacgtggt gaccagaata atgctgtgac ggaaaggcct gccgcgacgc acagcccaag
     6841 gacgaagaga gtatagaagc tggaccgatc ttgtggcacc gaccagccag gaatcgaatt
     6901 ggtggcaagg aggatatctc ctattgccgc aacgacgaac tgcccaacaa gcgcaaccgc
     6961 gacgcctgcc aatacgaaga tcagaccagc ggtggcggca agtagctttc ggttgcgtgg
     7021 gctcatggct ctatgacgca cagctatgta ttatctggtt ccgtataaga tgccaattgc
     7081 cttgtgacgc tggataaggc ccctgcggta tcctgccagg attgggacct cagtccaagt
     7141 ggttttcgga ggttcttctc tgcatctcat ttacgccggg tctgatatgg ttccgcataa
     7201 gcccccgcca tttccatagg cttcatagcc tatcagccgg gctccggact ccatgtgggc
     7261 cgcgattcag cccggcgtgc cggtgtatgc gatttcgatg actttcattt catgtttgaa
     7321 agtggcttgc gacgagtcgc ctaatgccat caagctcagc cgcgccgctt tccggcgtcg
     7381 gctgaagcgc agagttagag ctgttgatcc agaaggcaat agggccttca gttacccctt
     7441 gggtagattc gcgcgaccag ggccttgtcg ctctctgaga gatcctgcct tttaccaagt
     7501 attgttcctc ccgtccatgt cggagaaaac gtcatcgtca ttactgatcc gggatcgaag
     7561 tcacgatact cgcctagttg agcctcagaa acccgttgaa acacctgtct ctcgatctgt
     7621 tccttgctcc agaagttcgg tggcccgctt agctcgcgat ataccaaatc tctattccaa
     7681 tgaatgcgcg ctttcgggtt taggtgctct tcgatcaatc cgatagcgtg accaaactcg
     7741 tgcaacagtg ttctccggtc gatccattgg aaattcattg tcgcttgatc cttggcaact
     7801 gctaaggcgt ctgtacccaa aaaggaccat gaaccttcct gcttgaaaga cacccgaatc
     7861 tctgagtcac cagaagtgac gaactgaaat ctcaggttcg cgtacttggc ccactcctga
     7921 gcaacttgct tcaccgcttc atgagacttc gcatctccgt cgaggaataa aatgcggata
     7981 atggttccgt tcgcccaaaa cttgctccag gcgcctcgcc gaatcttgtc cggctgctct
     8041 gctctagcct tctgaagggc tgccgtctcg cgcggagtta actcccgaat agacaccacc
     8101 atagcaatag ccgccgcttt ctgttcggag atttccccga ggctactttt cgcttcggcc
     8161 aatactgcct ctacgtccct ctttagcact ttgacggtcg cttgagctgc ttctaggtca
     8221 cgctgtgctg ccgagagctt ttcttgggta gtcttgagtt gctcctgagt gtctcgaacc
     8281 ttttccagcg ccgattcgag tttgaacccg aagagataag caccgaccac caagaagagc
     8341 gctaggactg ctccagaaaa cttcccgaat cggatcaaat cgcggtccaa aacatatgtg
     8401 acccgtccat catcaagcgg tttctgctcg gagacttgaa cgggcaaaag catggaatga
     8461 cagaagcgac acttaatggc ctcatcattg atttgctctt tacagaatgg gcaagtcttc
     8521 atcgtgtagc tactctgcca gcgctctaac tgacggcgca tcagccgcgg cggctcagaa
     8581 tcgcagcagc cgccggcggc tgctgcgctt gttagccggt cggcctccgg ccatctggtt
     8641 tcgtcagctt gcctttcggc cgcaagccat cgaatgcgaa tggcagtccc ttgtcgattc
     8701 catagtgcag agttgcaacc aacatgctcc gcctcggagc cgaatctcgg tcctcgtcgc
     8761 tgaccgcgca ccactcgcat cggctgtcga cgagtgaact cggttcgccg cattcctcac
     8821 agatgtcgac caacgtggcg aagtcctcgc ccgaccattt cagtgtcctt cgctctgttc
     8881 tgaagaaggg gtcgtaggtt cgtttcccga ccttgaactc aagcagtggc actttgaatg
     8941 ccgcggctcg gcgacttgct aaggcccgca ggtcagggcc gacgttggaa ccaacgataa
     9001 tgaacctcac ggcctcggcg cttcacgctc ccgctatccc ctccgtccaa atccctcccc
     9061 cgtcatcacc tcggccacgt tctgcccgtt cacgaagaca tcggccaggg tccggccgta
     9121 gacatcgcgg ccgtggggga cgatgttgat gattccttct tgcatcagtt gctccagccg
     9181 aagggcagct tctcgcccgc ctggttcgga taattccggc gcgttgtacc cgcgaatgcg
     9241 gatgcgatct gtgccgtagc gaatcgtatc gccatcgacg gtgcggactt gccagggttc
     9301 cacggtcctc tcgaatcctc ccatcgacag cgcgtgacgc ggaagggagc gaaggcctcg
     9361 actatgaaaa cgcgattttt ttgcaaaggg attcttgtgc ggccgcgggc cccgctcgtg
     9421 ctgtccacgt cgataccgtg gcgcctgatg ttggggtagc ggctgacggt cctccggttt
     9481 caacccccaa caatcgttgc agtgaaacgg cccagggtcg tttgtggcgg ccggcgtata
     9541 ggtgtgcagg gtggaccggc tttcctccat cgcgctgagt gccggggtga gaggtgaagt
     9601 cacaaggagc aggaggacca accggctcac ggtgcgagag tcgagtatga tcgtgtgcag
     9661 catgcgaagg agccctacct ttttgacgac aggcaaattg tctcgatctg aaagaacgac
     9721 aaacgattag cgcgtaacgg ttcgttacct gctataggcc aggaattctc ctttgaggcc
     9781 tgtatccagg tcgagaagca gccgatgtaa attcatgccg ggcactgcag catgcttgcc
     9841 gcaatgggct ggcccctgcg gcaatgagga gatggatggg agagaatcat cgggttggcc
     9901 aggcccggca ggggtcattg agagcgaggc ttgtcgcgtg tgatcgtggg tcggttcact
     9961 cacaaacttt tggatcattt gatgggagcg ccatatcgaa atgagaagga gagcggcggc
    10021 caaaaacaca agaaatccag tgggctccgc cagcttgctc tgccggtctt ctccattcgg
    10081 gaaataccac agcgcgaatg tcagccaata catggcaatg aatgcgacat gcggggagaa
    10141 cgtgttcacg tccggcatga ccccgttcct ttgcatcgag gccgcaaagt gccaacacat
    10201 cgccgcgatg tgccagaggc tcagcgcgat taaaatctcg aagacatgct tccctgcatc
    10261 gatactcagt cccctgacaa gaatcaccat aagcagaaag gccatgatca aatcgagaac
    10321 gtatcgggca gcgctcaagc tggacacgtc gtatggaaaa ttcttgatga ggatgtgata
    10381 gacgcctatg tcatagatga caaatcccag gaatcccgcg gccatgaaca gatgcttcat
    10441 gaatgcggac actgaggatc tggcggtaag gcgcaatccc tcttcaagct tctgaaagcc
    10501 gaacccaatc actgaaccga agacgatgtc taggatccag acccatcgtt ctcccactgc
    10561 ctgtacgatc tccggctgca tggttcgctc catgacactg agtaagaagc atgctccatg
    10621 ccactagcgg attgctgagc tcgcgcggtt gacaagccga aaaaccgcgt gctcaaatgc
    10681 cttccgttga agagcgtatg gctaatggcc aatagcgaat agttgggact ttgggtttcc
    10741 ctcagctcgt accataagcc atacgctatg tgctcttaat cgttaaggcc gcatgaacta
    10801 ctacaggaga gccgctcatg gatatgtttg gaaaagtggg gctggtcgag gtgccgatct
    10861 tgatcgcccc ggatcaaaag gcctggatgg atcgcatgat caaggaaggc aagattgcga
    10921 ttcctcccgg cgggacgctg gaaaaaggct cgctctattc gatgtttgtc cgcatgctcc
    10981 tgcacaacgc gatggaagaa cagaagcggc aggaagcgtt ggagatggac gacgaggacg
    11041 acgacgaata gctcaggatg ctgaaaacgg tccccagcat cgttctcgca tcgctcggcc
    11101 cctcaacgta ccgcaagcgt acgccttggg gcctcgttcg ctgcggcctt gctgggaaac
    11161 cgttttgagc atcctgaagt cgtgcggtga attcgatggg aatgaatcgc gatctgtatc
    11221 tgaatggatg catttcgtat cggtttcgat acgaaatgca ggattcgcga acgacgcttc
    11281 acgagcgccg agcaacgccc ggcgctttct gccgggtcag aggttcttga acgcagcttg
    11341 agcggcgctg atcgtgcggt cgatgtcttt gggcgtatgg gccaccgaca tgaacgcggc
    11401 ctcaaactgt gacggggcca ggtagattcc ggcgtccagc atctggtgaa agaacttcgc
    11461 gtacgctttt gtgtcggctt tcttggctga tgcccaatcc gtgaccggcc cttgagtgaa
    11521 gaacgcacac atcatcgaac tgacgcgggt ttgcgtgagc ggaaccccgg cctttcgcgc
    11581 ggcattcccc actccctccg agagggccac cgaacgttgt tcgagctgtt cgtaggtgcc
    11641 tggtttcttg aggcgcttca gcgtttcgat gccggccgtg acggccagcg gattccctga
    11701 caacgtgccg gcctggtaga ccggtccgga cggggcaatc atcttcatga tgtccttcgc
    11761 gcccccgtag gcacccaccg gaagtccccc gccgataatt ttccccagga tcgtcagatc
    11821 cggcttgatg tcgtacagcg tctgtgcccc gccgtattgc acgcggaatc ccgaaatgac
    11881 ttcgtcaaaa atcagcaaca tgccatgggc atcggtcagc ttgcgcaagg cgtgcaagaa
    11941 ctccggcgag ggcggaatca cacccatgtt cccggcgatc ggttcgacaa tgacgcaggc
    12001 gagctgacgg taatgtctct tgatcagttt ctccgtggtg gccaggtcgt tatagggcgc
    12061 ggtcaacgtg tgtttggtga aatcttccgg gacaccgggg cagtcgggaa tgccaagtgt
    12121 ggtgagaccc gaccctgcct tggccagcag gtagtcgctg tggccgtgat aacacccttc
    12181 gaatttcagg atgccgtcgc gtttcgtata ggcgcgggcg acccgaaccg cgctcatgac
    12241 cgcttcggtt cccgaactca ccagccgcac catctccata gacggcaggg cctcgcgaat
    12301 catcgtggcc agttgaattt ccaactcggt cggcgcgcca tagctggtac cgtttccggc
    12361 ggcttgttga atggccttgg tgacggtcgc cggcgcatgg ccgagaatca tcggtcccca
    12421 ggacaggacg tagtcgatgt agctgttccc gtcgacgtcg tagagccgtg agcctttggc
    12481 gcgcgcaatg aaccgaggtt gcccgcccac cgagcgaaag gcccgaaccg ggctgttcac
    12541 cccgccggga atgatgagtt gcgcgtctgc gaagagttgt tccgatcgtt gtgttttcat
    12601 gggcctcttt catcgagccg tgagttgggg caccaaccta ccacgggaaa ataaaaacgg
    12661 tcaattgtgg acagtcttct gtgaatcggc accggtgcct ctactcatcc acgattcgat
    12721 acagccgatt atccgaatag atacacccac gatgattcgt agcggcataa ggcaatgtta
    12781 gcacagagaa acaaggagtt agagaccgcc gaccatatgg cgcaagtctt gctgagtctt
    12841 gggtatcact gcgtaggaga acacgatgaa agtaatctgt tcctggtgcc agagcgaagg
    12901 ccgaccgggg ttggtgcgag aaaaggctcc gttggctgat ctgcgcgaga cccacggaat
    12961 ctgctccgac cacctccagc aaatggcggt tggccgggat ccgctgatcc ccgggtatga
    13021 tgaaggctcg ctgccgcgcc atgaatctgt cgtcatccgt cgaagcgatc tggccgccgt
    13081 ggcctgttat gaatgagcaa gcgtgtgctt ggatgcgacc gtcccaccgt catcgctctt
    13141 ggattcccca acgagaacga caaaaaacgt caatcactgc cggaatgcca cgctattgac
    13201 ggcctcaacg gacaagcctg attggctcac gcgaacgatc ctgctgaaaa cattgaatac
    13261 ttcttggccg atgcccgctg gtgcgactct tgcgccccca acgtggatgg tcgcatcgca
    13321 acgaacggag tgaagtggtg aaagtgctct gtgcctggtg tatacgagac gggaagccgg
    13381 cgtttctgcg ggagaaattc ccgttggagg atcccagtga aacgcatggg ctctgtggtg
    13441 accatttcac ctcgctcagt gcgagtgtgg ggaaaatcgt cacaccgaga gtctggatgc
    13501 tctcacgcat gcacgacctc tcctggggac tgacgcggtg ggcccaacgg atgatggggc
    13561 ggttcttctc tctctgctag cgcggaaggg ccacccggca ccctgaaatg agtgccggga
    13621 atcgagtcct acccgagttt tcctcgcaag agattcaatc cctgttcaat caatgtggtg
    13681 tccggcatct tgccgtccgg ggtcagctta tcgatgagcc cgggaagcag attggccagt
    13741 tggctcccgg ctgcttccga gctcaatccc gcttgagatg ccagttgctt caggaggtcg
    13801 ccgccaaggc cctgctggat ctgctccgcc gagaccggga ggttttttcc tgtgctgacc
    13861 caggaattca cgatatctcc caggccgttc ttctgaaacg cctgcacgag tccactcagc
    13921 cccccgacgg aactgttctg ccctagcagg ttcactacgg cctgcatcag ggggttctta
    13981 tcgccgcccc ccatcatgcc ggctgccgcc tgtccgagtt gatcgagaag tcccatcgta
    14041 acctcctggc gaaattggat taggtcgact gtctatcgcg tgggtttttt cgcgcctttg
    14101 gccggggtgg atttcgatgg tttgcgctcc atggccaacg gcggcgcgaa ccaattgtgg
    14161 cggctccgtt tatggcgatc cgagacagcc agcacggcgt ggaacggcag cccgtctccc
    14221 gtcgcctcgt cggattcttt caggctccag gcttcaccgg cttcgctcaa gatctcaacg
    14281 aaggtttcaa agtcgtcttc ttcgtcgggt ttcgtcagta cttgatccgc atccgtcacc
    14341 accacgagat agcccttggc cggaagccag tccagatcgg ccaaacattc ttcgagggca
    14401 tcccagttgt ggccgaagta gtccggaaac gagaagaccc ggctgaattc atccagcagg
    14461 cccgccttcg ttttacattt cttgccggcg atggttttgg tgacgaagtg tgcggggacg
    14521 gagagaattt tatcgagcgc ctctccttcg gcatgcacca ggagatgcgc ccagggtttc
    14581 ttgatcgatt ggagggcaat gagcgacatg tctcctcaat tcatcggaac aaatgtccgg
    14641 tagtggtcgg cagtgtaata ggccttgccg gtgcgttggt cgatgacgat ccgttcagcg
    14701 cctcgattct tccccggcac cttggggtgc acatcgtact cgcgatagcg cccgcgcggc
    14761 agtacgcgtt cacgattctg aaaggtgcgt cctccgatat agcccggcgg cggatcgcca
    14821 tgccgcgttt caatagcgct cagcgtctct cgcgcaatga gcggtatgga tgcctggtcg
    14881 ctacgcgttg ttgcctgggg tccggcgcgc acggaaccgt tctgtgtctg cggcgtctga
    14941 tcaggagccg gtgtgaggat cgggggcggc gcatcgagga gtgtctggcc cggttctgtc
    15001 gcgaaggaga cgcccaggga gagagcgact gtggcgcaga gccaaagaag aagccgtgtg
    15061 atgcgtcgaa tggacatcat gattcgcaac cggtggcatc aacaggacga ccctaacatg
    15121 tcacatggga ggggtcaacg tgctacgccg tccgatcctc tcgtccggcg gggcttttct
    15181 cgttcggcct cctcgatggg ctgctatgga tggcgatatc aagaaccggc cggctcacca
    15241 actcgccggc gcgcacagac gtggcgcgat ttattcatcg cgccgtgcgc cctgcgagaa
    15301 gccccggcgg gcgtcagtct ctaacgcctc gcaacggcat tcatgaatca tccgagctag
    15361 accctgtcgg gggccaactc cgtcccgtgc atgcgggtaa ataactccca gtcgaacggt
    15421 ttgggcgcga cggcatggag aggaatcgtt tcgagccggt tggaatcgtg gtacgcgacc
    15481 atgcagccga cgatcgtgtc cggttgttcg acaagccggc ggacggtttc gtaggcgaga
    15541 tgctgcgcga tcattttatc ttccggtgtc gggggggcgc ccctgagcgt atgccccagg
    15601 atcgtcgcct tcgtggccgt ggccaatgca tagtggccgg acttctcccg acgttgcggc
    15661 caggttgcga tggtgttggc cacatagtcg accagcccat ggacaccgcc ctcccggtgg
    15721 tgccggtggg gagttcgctc tgccacgaca aagatgtggc tcttgttggg cacacccagg
    15781 gtacgcttca acgtgcccag gacaatttct tcaatgtagg catcggggtc ggggtgttca
    15841 ttcacgagga tcccttccgc ccgggcctga taggcgcagg ctaatgccag atggccggac
    15901 cccgctccca tgacctccac gaaaaagatg ctgcccatgg ctgcgctggt cgccttgagg
    15961 gattcaatgg attgattcgc cagcgcgatg gccgaatgaa accccaggga agtcgtgccg
    16021 gcgatgttgt tgtcgatggt gccgggaatg ccgacgacct gcaccccgta ggtctcgtga
    16081 atcgcccggg cgccccgcag gctgccgtcg cctcccagca ccacgagcgc cgagtctgcg
    16141 agataaggcg caagagaccg catggcggcg cgttggacct cctcgtcttt gaaatcttcg
    16201 aaacggctgc tgccgatggg gctgctggca tggctgccca tgccgcgtgt gtcttcttcc
    16261 gtgacgggat cgatccagtt gttcgccaat ccgaggaaac cgtgccggac gaaaaagacc
    16321 tccaggccga agcggtttcc ggtcacgcgc aattctttca acgccgctcc gccgccgccg
    16381 aaatccccgc ccgacacgag tgccagcagg cgcttgatgc gttgaggctt gagtgtgacg
    16441 cgctggctcc gctcccgctc gaccgtcacc caggcttcgc gggcgacccg ttcactttca
    16501 atcaggccga cggccgtgga gtagccggag agtttgccgt tttccagcgt gaggaggacg
    16561 acattgtcct gaccctcctt gtattcgccg aattcggaaa acgcttcgcg gaacggacgc
    16621 gggtccagat agatacgcag tgcgcgcagg gtcgaactgt gggtatagag gcagagcact
    16681 tctccgggat gggcacggcc cagccgatgc aggccatcga tcacatcgat atagaggtcg
    16741 aagaacgaat gcccgcccgg atagctatag aaggggtgtt tgatgagccg cttggcggtc
    16801 ttcgcatcga cgccgaaggc ctgcgcggca gcgtcgatct ccgcctgctt ttccatgccg
    16861 gtgacccagc cgaagtcctg cgactccaac gtggcctcga catggggatg gacggcatcc
    16921 gcacggtcgg gaatcaacgc cttcgtgatg cgccggcaga gttgctccgt gttcggacta
    16981 tggctgatga ggtgaaggaa cgtgcgcgga tccagataat tgcgcaactg cagataacct
    17041 aattgttgtc cgaccacgcc caccatgcgg gccagcgccg cgccgacggc ctccgccctg
    17101 ggtatcccgc gttcggcatc caacacattc gccagccgcc ggcccactct atgggtcttg
    17161 ctttcaactt ccgagacgcc atggcgcatc gtgagaaaca gcgtgcggtc gccgaggtcg
    17221 cggggaagca gccagaaccg gtcatcaccc agatccaaca caatgcggcc ctcggccaac
    17281 tgtggggtca actgagtgcg cggcacgatg gcgaccgggc gacgatgcgc aggcttctgc
    17341 gtggcgccga tcggcgcgcg atacaacggg gcaagttccc cgcgcgcgag aagcaggttc
    17401 catgcggcaa aaaagaccag cggatcgctt ccgcaggcgt cggttatgac tgcctggcgt
    17461 gccgtgcgat aggcctcggc gctggacaga ccggcctgcg cctgctgaat gaagattctg
    17521 atccggccga tcgcctcctc ggcgcgcggt agccggtcgg cagcaggcag ggggggaggc
    17581 ggcagtacga acctgccgct cgaggtgcgt tgcgcgaggg actccccata ggccagcaag
    17641 ccttgcatcg tgacaaagtc caactgatcg gttgactggc tgtccgaagc gctcatgcgt
    17701 agcctcgtgt gctggttcgt aagtaaattt taatgagact gagcgtggtt catcaccgaa
    17761 aagtatatgg atgttctttt gcggcatggt gacgggtttg ttagaatgac ccgttgccga
    17821 ccgaatcacg gatgcatcga gcctcaccgt tcacgtacaa gatggtgtgc cgggccaatc
    17881 ttgtcaaggc agaagaacag cgccatgcga aaagcgaaaa tcgtctgcac catcggtccg
    17941 gcgagcgatg gcgccgtcgt gctcgaccag ctcattcgaa gcggcatgga tgcggcccgt
    18001 ttgaattttt cccacggcac gcacgactcg catgggcggg cgatcaagac gattcgcgac
    18061 acggccgatc gtcacggggt ggcgattgcg atcattcagg atctgcaggg accgcgcatt
    18121 cgcgtcggcg aaatcgacgg tgcgctggag ctcatcgccg gtcagcgagt tcggctgagg
    18181 acgatgtcct tacgatccgg cgggcagatc ggtgccagga cggtgatcgc cgcgggtact
    18241 gcgcaggata tccctgtcac ctatcaggcg ctgaccaggg acattcggcc cggggccaaa
    18301 atactgatcg acgatggact ggtggaattg acggcggatc gaatcgtcga tggtgcggta
    18361 gagtgtaccg tgatggcagg cggacgtgtg acgtctcata aggggatgaa tctgcccggc
    18421 acggtcgtga gcgctccgac cctgacggag aaagaccggg aagatgtgcg tttcggcgtg
    18481 gcgcagggcg tggactatat cgcgttatcc tttgtccgtg gagcgcagga catcatggcc
    18541 gccaagcaat tgattgccga atgcggcggc gacgtgccgg tgatcgccaa gatcgagcgg
    18601 caggaggccg tcacggacct ggaggccatt ctcgttcacg ccgacggggt tatggtggcg
    18661 cgcggagatt tgggcgttga gctcggcccg gaagccgtgc cggtgctgca gaagcgcatc
    18721 atcgcgacgg cgaatcgcca acgacggctg gtgattaccg cgacgcagat gctcgagtcc
    18781 atgacacaac atttgcggcc gaccagggcg gaagcgtcgg atgtggccaa tgccgtgttc
    18841 gatggcaccg atgccgtgat gctctccgcg gaaacggccg tcggccatta cccggtggaa
    18901 gtcgtgcagg tgatggatcg g
//
LOCUS       DAORRQ010000072        18852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_172_length_18852_cov_83.019046, whole genome shotgun sequence.
ACCESSION   DAORRQ010000072 DAORRQ010000000
VERSION     DAORRQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18852
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172_length_18852_cov_83.019046"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK224_17225"
     CDS             <1..480
                     /locus_tag="PK224_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB17541.1"
                     /translation="VLFLCENNLYAMGTALARHQSQPDIAAKAKAYAMSADTVDGMDV
                     LAVETATGRAVELIRNGNGPYFLEYRTYRFRAHSMYDAELYRTKDEVSEWKRRDPIAT
                     FEQTLRQARLLRDEDLQQIEAAIETEIEDAVAFAEAAPWEPIEDLSKDVYTDVRRTT"
     gene            536..1510
                     /locus_tag="PK224_17230"
     CDS             536..1510
                     /locus_tag="PK224_17230"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRB17542.1"
                     /translation="MSKITYREAVRAGLRDALQSDPRVFLMGEDVGKYGGTYACSKGL
                     LDEFGPERIRDTPLSESTFVGAGIGAALGGMRPIVEVMTVNFSLLALDQILNNAATLR
                     HMSGGQFNVPLVVRMATGAGRQVAAQHSHSLEGWYAHIPGITVLTPATVTDARGMLFA
                     ALQEPDPVFIFEHAYLYSMEGELGENHPVVDISRAAVRRPGKDVSLITFGGSLWKALA
                     AATQLAQEGIEAEVMDLRVLRPLDIGTILTSVRKTHRAVVIDEAWRTGSFAAEVAAQI
                     MEGAFYDLDAPVARVCSEEVPIPYPKHLEEAALPQPDKIVKAVRTLLG"
     gene            1599..2771
                     /locus_tag="PK224_17235"
     CDS             1599..2771
                     /locus_tag="PK224_17235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRB17543.1"
                     /translation="MAEFLMPTLGADMTDGTLVQWKKKEGDRITKGEIIAEVDTEKAA
                     IDVESHTTGIIERLITRPGAKVPVGAVMAIIREEGRPTTSVASPRTVAVPPSPPSPVH
                     RTESGTVAPPQAGRLRISPAARKLAAERGIDASGLQGTGPEGAITLEDIERATTATAD
                     IAKPVGAADRQARMRQTIAAAMARSKREIPHYYLSTTIDMGRAITWLKDSNEQRPVTE
                     RLLYGVLLIKAVALALRRVPELNALWKDGEAGRSERIHIGTAISLRQGGLVAPALHDA
                     DRLSLSELMQNFQDLVKRARAGSLRSSELSDPTITVTSLGEQGVETVFGVIYPPQVAL
                     VGFGKVVERPWVADGLVVPRPVLMASLSADHRVTDGHRGGLFLAEIDRLLQEPQSL"
     gene            2768..3025
                     /locus_tag="PK224_17240"
     CDS             2768..3025
                     /locus_tag="PK224_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRB17544.1"
                     /translation="MSTPPSKGDTRSQVLRLLGEIAPEADTTSLRPDVSFRDQLDLDS
                     MDFLNFVVALHKEFHIEIPEADYPKYMTLNGCLTQLSAARS"
     gene            3022..3702
                     /locus_tag="PK224_17245"
     CDS             3022..3702
                     /locus_tag="PK224_17245"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRB17545.1"
                     /translation="MRDSATSSTDACILKVVVGSHAHGLAGPESDKDYRSVFVLPTAE
                     LFRVGFKYQSTRMMKEEADETSWEIGHFLQLALQGHPLVLETLVAPVVTMDDWGAELR
                     QLFPRLWSAQAAYESFLNYCDNQRKKMLEKKDGRPAKYAAAYVRVLFNLCELLERGTF
                     SIRISETPVGEAVARIKAGHFRPGEVIDIGEHWTEEAARRLRTCSQQARPELADAFLL
                     KVRKAFLR"
     gene            4307..4780
                     /locus_tag="PK224_17250"
     CDS             4307..4780
                     /locus_tag="PK224_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRB17546.1"
                     /translation="MSGLTRWEPFRRQLNPWRELEDMEQRLSALWGRTPTKAEGQKES
                     ISVAEWSPLVDITEDDKEYLIKAELPEIKKEDVKLTVQDNVLAISGERKYEKEEKNKK
                     YHRVERAYGSFLRSFTLPEDADGSKVAAEYKDGILTVHLPKSEKAKPKSIDVKVS"
     gene            4797..4946
                     /locus_tag="PK224_17255"
     CDS             4797..4946
                     /locus_tag="PK224_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17547.1"
                     /translation="MFRHPRYLDIPWEIHWHAEGASHHHRWLMVLVIVAALFLIGVGV
                     TSGLW"
     gene            4998..5651
                     /locus_tag="PK224_17260"
     CDS             4998..5651
                     /locus_tag="PK224_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HRB17548.1"
                     /translation="MFKNREEAGELLAQELIQFRKDPSAILLALPRGGVAVAYQLSLA
                     LHLALDVLITRKIGAPGNPEYALGAVSETGAVYWNREALLGLSLTERQLSAAVQAQQK
                     EVTRRVALYRQGRPFPDLNDRTVILVDDGLATGATFFASVATARQGNPRRVIGAIPVG
                     PRSTVEEARKLVDQLIVLRVPDPFYAVGNFYRDFEQVEDREVLQYLNLAEEAFVNRS"
     gene            5707..6201
                     /locus_tag="PK224_17265"
     CDS             5707..6201
                     /locus_tag="PK224_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HRB17549.1"
                     /translation="MSKQTEGGHAARHDRFVQEGQHDSYKMPGKLKEPTVCKTCGALF
                     HKGRWTWGTKPTGADEIVCPACLRIEDKNPKGFVTLKGAYKDQHRDQVMGLIHNAEAQ
                     EKKEYPLARIMTIENRAEGLVVLTTDTHLPRRIGEALKHAHHGELELQYDKDEDFIRV
                     TWIG"
     gene            6233..6784
                     /locus_tag="PK224_17270"
     CDS             6233..6784
                     /locus_tag="PK224_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRB17550.1"
                     /translation="MGGGERTWDRWRRVLVGGLLMVTLTGSPATAEMQLLPGAAADLD
                     QATVDEVLATFRQAEQALRAGDVDGIMALYSEQYNYHGLKKTDMRKVWTNLFDEFREL
                     SDTHHFSRMIRVGSGSKTIIEVTCTGSLSGLSKTGGLRVPIDSWYEEVHYMTFDDGQW
                     RIRGNVGDSPRFMPFGTSPHPLF"
     gene            7036..7893
                     /locus_tag="PK224_17275"
     CDS             7036..7893
                     /locus_tag="PK224_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB17551.1"
                     /translation="MRALIALDWSEQAFAAVREVSYLYDLHEVILVHGIDLGMFQYPI
                     VAEVSNMQGYDDFRKAMGKAGEQLLDHTSTLLPSEGLSITRVCEFGKPASLILDKARE
                     THADLIVIGARGRGRVGELVLGSVSHRVALHADCTTLIVKEREGPVKRVTVAIEGHED
                     GARIKAWLLSHPFKNPVDLTIVSVVRQIPSTDPFSLFPLQDWTGIAVRSAEDLVKNLA
                     ASVMNHRYTVGTQVTVGDPTDILTERAKSADLLVIGSHGRKGLERFLLGSISHALLHH
                     VPCPVLIVR"
     gene            7943..8305
                     /locus_tag="PK224_17280"
     CDS             7943..8305
                     /locus_tag="PK224_17280"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB17552.1"
                     /translation="MKTFSIFSVVCLVILVSTWATAQTNRGNPRDGQAIYEKQCLRCH
                     GDKLDGAGPDGQYLIVRPANFQSINSRAKTDWELLITIANGALFSPMHGYRGKLTDQQ
                     MLDVLSYIRSVATPEFIS"
     gene            8330..9532
                     /locus_tag="PK224_17285"
     CDS             8330..9532
                     /locus_tag="PK224_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRB17553.1"
                     /translation="MRRRRIFYAGLMFVCLWLLGSEVSIHASKPTADLEVFVRAGCPH
                     CEAAKAFLRELRQRRPALHILVRDVGQDQTALTRLETLARRQAVTLIGVPAFYLQGEL
                     IIGYQDAGTTGARLLALLDRAAPSLDSEPLSACRPTQPACSTPADHTSAGEDSILLPW
                     FGRLSYPDIGLPLFTLAIGLLDGFNPCAMWVLLFLLSLLVSLQDRVKMSLVAGTFVLV
                     SGLVYFAFMAAWLNVFLIVGLSRTVQLTLGGVALLIGAVNVKDFFVFRQGLSLSIPAA
                     AKPGLYARLRRIVQAENLTGALIGIVILAGLVNVVELLCTAGFPALYTHILSTQALAT
                     WQYYGYLGLYNAAYIFDDALMVTIAVITLGRHKLEEREGRWLKLISGAVMIGLGLLLL
                     VKPDLLSG"
     gene            9536..12286
                     /locus_tag="PK224_17290"
     CDS             9536..12286
                     /locus_tag="PK224_17290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB17554.1"
                     /translation="MQRIRPLHMPPSRDEGPDSGHVVLSDGSTALLRIAQPSDADELQ
                     HFVERLSPEARRHRFFSETAPPAEVIRTLCDPSDPRRSLTVIALRRQDGALRVIASGS
                     YHARDPHQAEVAMAVDDRLHGHGLGTLLLERLALLAIRHGFTRLWAITHADNVAMREV
                     FASSGLPMEEHVEGGDMEVELSLTPTDHSVRQSEWRERVATTASLRPLFHPQAVAVIG
                     ASRAPQSIGYRLLDALSSNGFHGRCYAINPHAATIAGMHTYPSLRALPEPVDLAVIAV
                     PKDAVISVVDDCAATGVRALVVITAGFAEVGAEGRRLQDQLLEKVRQQGLRMVGPNCF
                     GILNTDPAVRLNATFTSTFPLAGSIAMSSQSGALGLALLAASERLQLGLSTFVSVGNK
                     ADVSVNDLLQYWENDPATNVILLYVESFGNPRRFAQIARRVSRNKPIVVLKAGRTSSG
                     KRAAGSHTAALAANDVGVEALFQQTGILRAETLEDMFALAAGLSDQPLPKGNRVGIIT
                     NAGGPAILCADACEASGLEVPELSQATMTHLASFLPPAAALRNPVDLIASADPEQYAQ
                     AIAALLKSDDIDALIILYIAVTATDVDPIAEGIKKGILEARATASIKKPIYIGWMVET
                     DRERRFSFTNDTIPTFALPELPARALGKIAGYVQWRERPAGMVPDFDDLDLPTVTRIC
                     HDAMAARGDGWLTTAETRALLSAMSIPLPPGGVATDAEEAALLAEQIGFPVAVKLASH
                     TLVHKTEIGGVHLNLNTKAEVRRAFEQIAARLAQDQSLDAMEGVLVQPMVTGGVEVMA
                     GMVQDPSFGPLIGFGLGGIHVEILGDVRFRITPLTELDAADLIRSIKGYRLLQGYRGH
                     PPGDVDAIQELLLRLSRLVEEVPEIVELDLNPIFAMPPGQGCRIVDARIRVGRSVARW
                     NG"
     gene            complement(12327..12842)
                     /locus_tag="PK224_17295"
     CDS             complement(12327..12842)
                     /locus_tag="PK224_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17555.1"
                     /translation="MFNDPEWASKEEWDVYAWFGFALSEAQTIEMLLQVLVTALSLRG
                     TDKHHDWPTLYDGFGKLRLRDLFKEVKSHKNVQLSETLLLNLTEARKVRNRIVHGFFR
                     PSDSPLSEAMRLTDAIRELQMAASLFRNCVPELERVVYEALDQLGVTRAEVEAKISKR
                     SAEMNGQGSVD"
     gene            complement(12944..13294)
                     /locus_tag="PK224_17300"
     CDS             complement(12944..13294)
                     /locus_tag="PK224_17300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HRB17556.1"
                     /translation="MKKVTTTKYDVAEHLRTPEEMAAYLEACLEEADGDAAFIAKALG
                     DIARAKGMAQVAQDAGLSRESLYKALSGERSPGFDTILKVLRALGLKLHAEPTRRIAA
                     PKSKRPRPFNRSAA"
     gene            complement(13304..13606)
                     /locus_tag="PK224_17305"
     CDS             complement(13304..13606)
                     /locus_tag="PK224_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB17557.1"
                     /translation="MQFCRFEIRKTKTFAKWLDGLRDMRARARVQVRIERLGTGNAGD
                     AKSVGEGVSEMRIDYGPGYRVYFTKRGSEVIVLLAGGDKRTQDADIRTALRLARSL"
     gene            complement(13758..14189)
                     /locus_tag="PK224_17310"
     CDS             complement(13758..14189)
                     /locus_tag="PK224_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17558.1"
                     /translation="MQLNLEQAFPTEQALRDMIVMRKVCFDHDPFYVKTGAGNVVQIG
                     FQLNLYAAFRDPTQLPLNDDAEHREILGDLQRLCRVFFQTLDLLKPCEHPQPPAHRIV
                     FSPERKNRAEAVSRSRSSTWRIMPTARLARCRIFLRRRNVC"
     gene            complement(14211..14765)
                     /locus_tag="PK224_17315"
     CDS             complement(14211..14765)
                     /locus_tag="PK224_17315"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRB17559.1"
                     /translation="MRYHPLPRTAVPVVALTTLVLLVWIGWGSVRSPASFWAPGELSR
                     YHVDQAGCLHCHEPFRGPSPARCVSCHSDRYFADRATPAVTTWHRDLVAQRTACTACH
                     TEHRGALAQITERTRTNPHGEFVFRATGTSSCTACHEFGARVATKPTLRDEPIVRQLY
                     ERGRGAHQPGRIAECLACHGRGAS"
     gene            complement(14769..15356)
                     /locus_tag="PK224_17320"
     CDS             complement(14769..15356)
                     /locus_tag="PK224_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17560.1"
                     /translation="MTSVRLRVLVVTVVVVAAVAGALIFEALLSLGETAPFGHTQYGH
                     AAGRVGLVVTLLVFVYPLRKRISPARRWPRGWFRVHMVAGVVGPLFIFLHSGAHYHAL
                     VPILAMLSMVIVVVSGVVGQAVHAFSLSALNDQRRRLQHEGLSDSDIDAQLHGMASQE
                     EAFRMWQAIHAPMTLMFLVFTLLHVAGALFFAGIS"
     gene            complement(15457..15843)
                     /locus_tag="PK224_17325"
     CDS             complement(15457..15843)
                     /locus_tag="PK224_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17561.1"
                     /translation="MSRPGLGAALCILGVLVVASVPRWTYAVPYGAVITSEAEGPSIA
                     WTIADAGGMVANPHSDLIFRATGAATCLDCHRAGKEGTVSPQVQDNALVQELKAKAKG
                     VHGPGRFADCLRCHAGGNKGVEKYRK"
     gene            complement(15840..16322)
                     /locus_tag="PK224_17330"
     CDS             complement(15840..16322)
                     /locus_tag="PK224_17330"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB17562.1"
                     /translation="MSDGKISVTFEQDHDRLDALFTTFQEQKRKDFAKAKEAFVAFKF
                     GLQRHIVWEEDVLFPKWEENSGMAEGGPTQVMRTEHRMIGDCLEAIHQKVQAQDPESD
                     RDEQRLLDILKSHNMKEERILYPSIDQVISDQERAELYQAMKDIPEERYRTCCGSGHS
                     "
     gene            complement(16337..16813)
                     /locus_tag="PK224_17335"
     CDS             complement(16337..16813)
                     /locus_tag="PK224_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17563.1"
                     /translation="MIRQAALPLLLFAALLVSGCAEPKAASGINGVTPVHLPDVRTPV
                     QLDAEARQEHRAVMLQHLETVQAIVAALADEDYRLAQGLTETHLGFFMHRHAMARQQP
                     ENFPPAYHDLAMAHHEAAEKLADAMPSHDLKQILPEFNKVLKACVACHLEYKLRSS"
     gene            complement(16810..17748)
                     /locus_tag="PK224_17340"
     CDS             complement(16810..17748)
                     /locus_tag="PK224_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB17564.1"
                     /translation="MWQRETFKKIKQVPGEEHVLPGSALCAGCGGLESLRLAAKVLGD
                     EVVYVNAAGCFTMLAAYPYTSFKGSWLYTTMGSAPAGAQGVRDALDVLIAKGRLPKRE
                     NLKVVVLGGDGSTYDMALSSTSGAMNRGLDFYYICYDNEAYGNTGMQLSPATPYGART
                     ATSPCSLQHPSGASQEKKDIFEIWRAHKPPYIATVAPRYPLDLEEKFARAAAFTGPKM
                     FLMLAACPTGWLYDPGETPEVAKLAVETGLWPLKEAIDGVVTHTYVPKRKPVEAYLKL
                     QGRFRHLFEPTMQTEAIQHIQARVDAYWERAIMEES"
     gene            complement(17812..>18852)
                     /locus_tag="PK224_17345"
     CDS             complement(17812..>18852)
                     /locus_tag="PK224_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HRB17565.1"
                     /translation="AAEATGARVFTATSSQGLLYAFEVLYSISGWRAPLVLVNVSRAL
                     AAPITLESDHNDVLAARDAGFLQIHAETCQEVLDSILMAYRIAEDERVMLPVIVNLDG
                     FSLSFTREPVRIPDPEQVQTFLPPFRPAHLGFSASAPHSLGVAVIGGTPYAYFRHQMH
                     LASINALEVHREAAASFEQVFGRRYDVVEGYRLDDAEDVLVMTNSCSSTGKAAVDAAR
                     GEGKRVGLLRLRMIRPWPADAIRDALRGRRAVAVLDQNLAPGQGGILFQEIAASLYHE
                     VERPRALCSFIGGLGGQNLSRGAFHAIFEQTAKAGVSGKGIGPVLLYTQAEHREMTQL
                     QMVAAGSVKRET"
BASE COUNT         3858 a         5958 c         5439 g         3597 t
ORIGIN      
        1 gtgctgtttc tctgtgaaaa taacctctat gcgatgggca ccgccctggc ccgccatcaa
       61 tcgcaacccg atattgccgc caaggcgaag gcctatgcga tgagcgccga cacagtcgac
      121 ggtatggacg tgctggcggt tgagacggcg accggtcgcg cagtcgagct gatccgaaac
      181 ggcaacggcc cctacttcct tgaataccgg acctatcgct ttcgcgccca ttccatgtac
      241 gacgccgagt tgtatcggac caaagacgaa gtcagcgaat ggaagcggcg agaccccatt
      301 gcgaccttcg aacagaccct tcgccaggcc cgattgctgc gcgatgagga tctccaacag
      361 atagaggctg cgatcgagac tgaaatcgag gacgccgtgg ccttcgccga agcggccccc
      421 tgggagccga tcgaggatct cagcaaggat gtgtacacgg acgtgagacg tacgacgtga
      481 cacgtcaggc gaatttacgg agccgggtca tagctcatct ttcgcatcga tacctatgag
      541 caagatcacc tacagagaag ccgttcgggc gggattacgg gacgcgctcc aaagcgaccc
      601 tcgcgtcttc ctcatggggg aggatgtcgg gaaatacggc ggcacctatg cctgcagcaa
      661 ggggttgctc gacgagttcg ggccggaacg cattcgtgat acgccgctgt ctgaaagcac
      721 cttcgtcggc gccggcatcg gcgcagccct gggcggcatg cgtccgattg tggaagtgat
      781 gacggtgaac ttcagcctgc tggcactcga tcaaattctc aacaacgcgg cgactctccg
      841 gcacatgtcc ggcggtcagt tcaacgttcc gctggtcgtt cgaatggcca ccggcgccgg
      901 ccgccaggtg gcggcgcaac attcgcacag cctggaaggc tggtatgcgc acattcccgg
      961 catcacggtc ctgacgcctg ccactgtgac ggatgcgcgg gggatgttgt tcgccgcgct
     1021 ccaggagccg gacccggtgt ttattttcga gcatgcgtat ctctattcca tggaaggcga
     1081 attgggggaa aaccatccgg tagtcgatat ttcccgcgct gcggtgcgcc gacccggaaa
     1141 ggatgtgagc ctcatcacat tcggaggcag cctgtggaag gcgttggccg ctgccacaca
     1201 actggctcaa gagggcatcg aggcagaggt tatggatctg cgcgtcctgc gccccctcga
     1261 tatcggcacc atcctcacct ccgttcgaaa gactcaccgt gcggtggtca tcgacgaggc
     1321 ttggcgcacg ggaagtttcg ccgccgaagt cgccgcgcag atcatggagg gtgcgtttta
     1381 cgatttggat gccccggtcg cgcgggtctg cagcgaagaa gtcccgattc cttatccgaa
     1441 acatctggaa gaggcggcgc tgccgcagcc ggacaagatc gtgaaggcgg ttcgcacgtt
     1501 gctgggataa ccgatgcctt ggctgtttct ctgtcactca tggcccgttc cattgtacgt
     1561 gtcacgtttc acctttcgcg cttcacggag tctccatcat ggctgaattc ctgatgccga
     1621 cactcggtgc cgacatgacc gacggcacct tggtgcagtg gaaaaagaaa gaaggcgacc
     1681 gcatcaccaa aggcgagatc atcgcggagg tcgacacgga gaaggccgcc atcgacgtcg
     1741 agtcccatac cacaggcatc atcgaacgac tcatcaccag accgggagcc aaggttccgg
     1801 tgggagcggt catggcgatc attcgcgaag agggacggcc gacaacaagt gtcgcttctc
     1861 cccggacggt cgccgttcca ccgtcccccc cttcgcccgt ccatcgaacg gagagcggaa
     1921 ccgttgcgcc acctcaggcc gggcggttgc gaatttcacc ggcggcaaga aaactggccg
     1981 ccgagcgagg catcgacgca agcgggttgc agggaaccgg cccggaagga gccatcaccc
     2041 tggaagatat tgagcgagcc accacggcaa cggcggacat cgccaaaccg gtcggtgccg
     2101 ccgaccgcca ggcacgcatg cggcaaacca tcgccgcagc catggcccga tccaaacgcg
     2161 agatccccca ctattacctc agcaccacca tcgacatggg ccgggcaatc acctggctga
     2221 aggactcgaa cgagcagcgt cccgtcacgg agcgtctcct ctacggtgtc ttgctgatca
     2281 aagcggtcgc cctcgcgctc cgccgagttc ccgagctcaa cgcactgtgg aaggacggcg
     2341 aggccggccg gagcgaacgg attcatatcg gcaccgccat ttccctccgc cagggtggac
     2401 tggtcgcgcc ggcgttgcac gacgccgacc ggctgagcct cagcgaattg atgcagaact
     2461 ttcaagacct ggtgaagcgc gcccgggccg gttcgttacg gagctcggaa ctgtccgacc
     2521 cgaccattac cgtcacgagt ctgggggaac agggagtgga aactgtgttc ggagtcattt
     2581 atcctcccca ggtcgcgctg gtcggattcg gcaaagtcgt cgaacgcccc tgggttgccg
     2641 atggcctggt tgtgccgagg ccggttctca tggcgtcgct ctccgccgac caccgcgtca
     2701 cggacggcca ccgtggagga ttgttcctgg cagagatcga tcgcctgtta caggagcccc
     2761 aatcattatg agcacgccac cgtcgaaagg cgacacgcgc agccaggtcc ttcgtcttct
     2821 cggtgagatc gcaccggaag cggacacaac ctccctacgc ccggatgtca gcttccgcga
     2881 ccaactcgac ctcgactcga tggacttcct caacttcgtg gtggcgcttc acaaggagtt
     2941 ccatatcgag attcctgaag ccgactaccc caaatacatg accttgaacg gctgtcttac
     3001 ccaactctcc gccgcgcgat catgagagac tcagcgacgt cgtcgaccga tgcctgcatt
     3061 ttgaaagtgg tggtcggttc gcacgcccac ggcctggccg gtcccgaaag cgacaaggac
     3121 taccgcagtg tcttcgtcct gcccaccgcc gaactgtttc gtgtcgggtt taagtatcag
     3181 agcacgagga tgatgaagga ggaagcggac gaaacgtcct gggagatcgg gcactttctc
     3241 caattggccc tgcagggtca tcccctggtg ctcgaaacac tggtggcgcc ggtggtgacg
     3301 atggatgatt ggggcgcgga gctacgccaa ctctttccgc gtctctggtc ggcacaggcg
     3361 gcctatgagt cgttcctcaa ctactgcgac aaccagcgca agaaaatgtt ggagaaaaag
     3421 gatggacggc ccgccaaata cgcggcggcc tatgtgcggg tgctcttcaa cctctgtgaa
     3481 ttgctggaac gggggacgtt cagcatcaga atttccgaaa cgcctgtggg tgaagccgta
     3541 gccaggatca aagccgggca ttttcgtccc ggagaagtca tcgacatagg ggaacactgg
     3601 acagaagaag cggcgcgccg actccgcacc tgctcccaac aagcccgccc ggaactggcc
     3661 gacgcgttcc tcctcaaggt tcgaaaggcg tttctccgct agccgggcct gcggctcctc
     3721 ttccggacaa ccatcataaa aaaacccgct cccgtgtaga gatcggacct gcccactgag
     3781 cgggatccct ccggacaccc cggcgagtca cacagcctga atgtcccgcg ctccccattc
     3841 atcgggctcc tgatgcgagc cgtgcctcct actggcaccg agatggaagc gcgtcccttc
     3901 ggaccagacc ccgaaattgc gacaggcgcg acccccgaac ccactgacgc ggcacggaag
     3961 cgggggtttc gaccttatcc tggatccacc tcccttcatt tcccttcatt catcgggtaa
     4021 ccatggccgg attgaaatca atgaggtaat tggcaccatt gcgtcccccg tccgctgtcc
     4081 cgtaacgccc caccccgcag aatggtcggc gcacgaaact gcctgtcatt ctcctaagca
     4141 agtgaacttc cgcacctccc ctgtgaatgc cccccacatt ttccgctggc atcgagcctg
     4201 caagagtgcc aatgcagagt gacggttcca gaacaccatc tctcgaacgc catgacaaga
     4261 caccagctca ggctgaactt actcgtaccg taaaggagac atgaccatga gtggactcac
     4321 ccgttgggaa ccatttcgga gacagttgaa tccgtggcga gaacttgagg acatggagca
     4381 gcgcctgtcc gccctctggg gtcggacacc gaccaaagcg gaaggccaga aggagtcgat
     4441 ttcggtcgca gaatggtctc ccctggtcga catcaccgaa gacgacaagg agtatctgat
     4501 caaggcggag ctgcccgaga tcaagaagga agacgtgaaa ctcacggtgc aggacaatgt
     4561 gctcgcgatc tccggcgagc gcaaatacga gaaggaagag aagaacaaga aatatcatcg
     4621 cgtggagcgc gcatacggca gcttcttgcg aagcttcact ctgccggaag atgccgacgg
     4681 cagcaaggtc gcggcagaat acaaagacgg catcctcacg gtgcatctgc cgaaatcgga
     4741 gaaagccaag cccaaatcga ttgacgtgaa ggtgtcctaa gcggaaggag cacaccatgt
     4801 tccgacatcc tcgatatctg gacatcccct gggaaatcca ttggcatgcc gagggtgcat
     4861 ctcaccatca ccggtggctg atggtgctcg tgattgtcgc ggcgctgttc ctcatcggcg
     4921 taggggtcac gagcgggctg tggtaacccc cttcgggcca cagtatgcag ggcacacggc
     4981 agggggagcg gagagccatg ttcaagaacc gtgaagaagc cggcgaactc ctcgcgcagg
     5041 agctgatcca gtttcgcaaa gatccaagcg ccattctcct cgcgttgccg cgcgggggag
     5101 tggcagtggc ctatcaattg agcctggccc tgcatctggc gctcgatgtg ctcattacgc
     5161 gaaaaatcgg cgcgccgggc aatcctgaat atgccctggg agccgtcagc gaaacaggcg
     5221 ccgtgtactg gaatcgagag gcgctgttgg gcttgtcgct cacagaacga caactctcag
     5281 ccgccgtgca ggcgcagcaa aaggaagtca ccaggcgtgt agccttgtac cggcaggggc
     5341 gaccgttccc cgacctgaac gaccggacgg tgattctcgt cgatgacggc ctcgccacag
     5401 gcgcgacgtt ctttgcctcc gttgcgaccg cccgccaggg aaacccgcgc cgcgtgatcg
     5461 gggccattcc ggtgggcccg cgctcgaccg tggaggaggc gcggaaactg gtcgaccaac
     5521 tgatcgtact acgggtaccg gatccgtttt atgccgtcgg aaacttctac cgggactttg
     5581 aacaggtcga agaccgggag gtgctgcaat atctcaatct ggcggaagaa gcgtttgtca
     5641 acagatcgta acatcacaag acgggccaca gagcggatga cccggcccag aaaaggaatc
     5701 gcaccgatga gcaaacaaac cgaaggcggt cacgccgcgc gccatgatcg attcgtacag
     5761 gaaggtcaac acgactccta caagatgccg ggcaagttga aggaacccac ggtctgtaag
     5821 acttgcgggg cgctgttcca taaggggcgc tggacctggg gaaccaaacc gacgggggcc
     5881 gatgagatcg tctgtcccgc ttgtctgcgc atcgaggaca aaaacccgaa agggttcgtc
     5941 accctcaagg gagcctacaa agaccagcac cgggaccaag tcatgggcct gatccacaac
     6001 gccgaggcgc aggaaaaaaa agagtacccg ctggcccgca ttatgacgat cgaaaaccgg
     6061 gcagaggggc ttgtcgtcct caccaccgat acccacttgc cccggcgcat cggcgaagcg
     6121 ctgaaacacg cgcaccacgg cgaattggaa ctgcaatacg acaaggatga agattttatc
     6181 cgcgtcacat ggatcgggta gacaacctca ccatcggcaa gaaggagtgg tcatgggcgg
     6241 aggggaacgg acgtgggatc gatggcgcag ggttctcgtc ggcggacttc tgatggtcac
     6301 cctgacgggg tctccggcaa cagccgagat gcagctcctt ccgggggcgg cagcagacct
     6361 cgaccaagcc accgtcgatg aagtgttggc gacgttccgg caggccgaac aggcgcttcg
     6421 tgccggcgac gtcgatggca tcatggctct gtattcggag cagtacaatt atcacgggct
     6481 gaagaagacc gacatgcgga aggtctggac caacctgttc gatgaattcc gggaactgtc
     6541 cgatacccat catttctccc ggatgatcag ggtcgggtcc ggatccaaaa ccatcatcga
     6601 ggtcacctgc acaggcagtt tgtcgggcct gtcgaaaacc ggcgggttac gtgtgccgat
     6661 cgacagttgg tatgaggaag tgcactacat gacgttcgat gacggtcagt ggcgcattcg
     6721 cggcaacgtc ggcgattccc cgcggttcat gcccttcggc acgtcaccgc atccgttgtt
     6781 ctagaaggct gcgggaacac tgtccgcaga accagtgtga agaagaggcc aggcatgctg
     6841 gaatggagat gaatattttc gcagggtgct caagaattcc gccatcttcg tccgtcgttc
     6901 gtgaaacgtc gttcgatcct cgctgccaaa ccggctgtgg caggttcggg agatacgcgt
     6961 cacgattcac gcttcacggg tgacgggagc gacgctggag gactgtttca ggatcctgcc
     7021 ggcgaaagga gtcttatgcg tgccctcatc gcactcgact ggtcggaaca ggcgttcgca
     7081 gccgttcggg aagtctccta tctgtacgac ctgcacgagg tcatcctggt gcacggtatc
     7141 gacctgggta tgtttcagta tccgatcgtc gcagaagtga gcaacatgca ggggtacgac
     7201 gacttccgga aggcaatggg gaaagcgggt gagcagttgc tcgaccacac atccaccctg
     7261 ctgccctccg agggcctctc catcacacgg gtgtgcgagt ttggcaaacc ggcatcgctg
     7321 attctggaca aggccaggga aacgcatgca gacctgatcg tgatcggagc gcgcggacgg
     7381 ggacgcgtcg gcgaattggt cctcggcagc gtctcccatc gcgtcgcgct ccatgcagac
     7441 tgcacgacct tgatcgtgaa agagcgcgag gggccggtca aacgtgtgac ggtcgccatc
     7501 gaaggacatg aggatggcgc ccgtatcaag gcctggctcc tcagccatcc gttcaagaat
     7561 ccggtcgatc tcaccatcgt gagcgtcgta cgccagattc cgtccaccga tccgttcagc
     7621 ctctttccgc ttcaggattg gaccgggatc gccgtacgct cggccgaaga cctggtgaag
     7681 aacttggcgg cctcggtcat gaatcaccgc tatacggtcg gcacccaggt gaccgtcggc
     7741 gatccgaccg acatcctgac ggagcgggcc aagtcggcgg atctcctggt catcggctcc
     7801 catggacgca aggggctgga gcgatttctc ctgggaagca tctcccacgc cctcttgcat
     7861 cacgtgccct gtccggtctt gatcgtccga tgagtctgtg accaatccgg tgcactgcgc
     7921 cgaaccataa aggagcctgc gtatgaaaac gttcagcatt ttctctgtcg tctgcctcgt
     7981 catcctggtc agcacatggg ccaccgcaca aaccaaccgg ggcaatccgc gcgacggcca
     8041 ggccatctat gaaaaacaat gtctgcgttg ccacggagac aaactggacg gggccgggcc
     8101 ggacggccaa taccttattg tgaggcccgc gaattttcaa tccatcaact cgcgtgccaa
     8161 aaccgattgg gaactgctga tcacgatcgc gaacggagca ctcttcagcc cgatgcacgg
     8221 gtatcgcggg aagctgaccg accaacagat gctggacgtc ttgtcctata tccgatcggt
     8281 cgcgacaccc gaattcatca gttagccgac aggcgccaca ggcacagcga tgcgtcggcg
     8341 gaggattttt tacgcaggcc tgatgttcgt ctgcctgtgg ctcctcggtt cagaggtgtc
     8401 cattcacgcc tccaaaccga ctgcggacct ggaagtcttc gtccgcgccg gctgtccgca
     8461 ttgcgaagcc gccaaggcat ttcttcgcga gcttcggcaa cgtcgtcccg ccctgcacat
     8521 tctggttcgg gatgtcggtc aggatcagac cgcgctcacc cgtttagaga cgttggcaag
     8581 gcggcaggcg gtgacgctga tcggcgttcc ggctttttat ctgcaaggcg aattgatcat
     8641 cggctatcaa gatgccggga ccaccggcgc ccgcctgctt gcactccttg atcgggcggc
     8701 tccctccctg gattccgaac cgttgtccgc ctgccgcccg acacaaccgg cctgcagcac
     8761 gcctgcagat cacacctccg ccggagaaga cagcatcctg ctcccctggt tcggccgcct
     8821 cagttatccg gacatcgggc tccccctctt cacgctcgcc atcgggctgc tcgacggctt
     8881 caatccctgc gcgatgtggg tgctgttgtt tctcctgtcg ctgttggtct cgttgcagga
     8941 ccgcgtcaag atgtcgctcg tggccggcac cttcgtcctg gtgagcgggt tggtgtattt
     9001 tgccttcatg gccgcctggc tgaatgtgtt tctcatcgtg ggcctctccc gcacggtcca
     9061 actcacgctg ggcggcgtgg cgctcctgat cggcgcggtc aacgtgaaag atttcttcgt
     9121 cttccggcag gggttgtcat tgagtattcc ggccgcggcc aaaccgggac tctatgcgcg
     9181 gctgcgccgg atcgttcagg cggagaatct caccggcgcc ctgatcggaa tcgtgatcct
     9241 tgcgggactg gtgaacgtgg tcgagctact gtgcacggcc ggatttccag cgctctacac
     9301 gcacatcctc agcacgcagg cgctcgccac ctggcagtat tatggatatc ttggcctcta
     9361 caacgcggcg tacatcttcg atgacgcgct gatggtgacg atcgcggtga tcaccctggg
     9421 ccgtcacaag ctggaggaac gcgaaggacg atggttgaaa ctgatcagcg gggcggtcat
     9481 gatcgggctc ggcctgctgc tgctcgtaaa accggacctg ctctctggat agtgtatgca
     9541 acgtatccgc ccacttcaca tgccgccctc ccgtgacgag ggtcccgact ccggtcatgt
     9601 cgttttaagc gacggttcga cggcgctcct gcgcatcgcg cagccgtccg acgcggatga
     9661 actgcagcac ttcgtcgaac gcctgtcgcc ggaagccaga cgccaccggt ttttctccga
     9721 aactgcgccg cccgccgagg tgatccgaac actctgcgac ccatcagacc cgcggcgtag
     9781 cctcaccgtg attgcccttc gccggcagga cggcgccctc cgtgtcatcg cgtccggttc
     9841 gtatcatgcg cgggacccgc accaggccga agtggccatg gccgtggacg atcggctcca
     9901 tgggcatggc ttgggcacgc tgctcctgga gcgactggcg ctcctggcga tccgccacgg
     9961 cttcacgaga ctctgggcca tcacccacgc cgacaatgtg gccatgcgcg aggtgttcgc
    10021 ctcctccgga ctgcccatgg aagaacatgt cgaaggcggc gacatggagg tcgagctctc
    10081 gctgacaccg accgaccaca gcgtacgtca atccgagtgg cgtgaacggg tcgccaccac
    10141 agcttcgctc cggcccctct tccatcctca ggccgtcgcc gtgatcggtg cctcgcgcgc
    10201 tccccagagt atcggctacc ggttgcttga cgcgctgagc agcaatggct ttcacggccg
    10261 ctgttacgca atcaatccgc atgccgccac tatcgccggc atgcatacct atccgtcgct
    10321 tcgggcattg ccggagccgg tcgatctggc cgtcatcgcc gtccccaaag acgccgtcat
    10381 ctctgttgtg gacgactgtg cggcgaccgg tgtccgcgcg ctggtggtca tcaccgcggg
    10441 gttcgccgaa gtcggagcag agggacgccg tttacaagac cagctcctcg aaaaggttcg
    10501 ccaacagggc ctgcgcatgg tcggccccaa ttgcttcggg atcttgaata ccgatccggc
    10561 cgtgcggctc aatgctactt tcacctccac gtttcccctc gccggctcca ttgccatgtc
    10621 gtcacaaagc ggcgccctgg ggttggccct gctcgccgcg tccgaacgat tgcagctcgg
    10681 tctttccacc ttcgtcagcg tcggcaacaa ggcggatgtg tcggtcaacg acctgctgca
    10741 atactgggag aacgatcccg cgacgaacgt catcctgctg tatgtggaat cgttcggcaa
    10801 tccgagacgg tttgcgcaga tcgcccgccg ggtcagtcgc aacaaaccga tcgtcgtcct
    10861 gaaggcaggc cgtacctctt caggcaaacg cgccgcggga tcccacacgg ctgcgctggc
    10921 cgccaatgat gtcggggtgg aagccctgtt tcagcagacc gggatcctgc gcgccgagac
    10981 cctcgaagac atgttcgcgt tggcggcagg cctctcggat caaccgctgc cgaagggcaa
    11041 ccgcgtcggg atcatcacga acgccggcgg gccggccatc ctctgcgccg atgcctgtga
    11101 agcgagcggc ctggaggttc ctgaattgtc gcaagcgacc atgacgcacc tagcctcatt
    11161 tcttccgccg gctgcggccc tgcgcaaccc ggtcgatctg atcgcctcgg ccgatcccga
    11221 acaatacgcc caagccattg cggcgctctt gaaatccgac gatatcgatg ccctcatcat
    11281 tctctacatt gccgtcaccg ccacggatgt ggacccgatc gccgaaggca tcaagaaggg
    11341 catcctcgaa gcgcgagcca cggcctccat caagaaaccg atctacatcg ggtggatggt
    11401 ggaaacggat cgcgagcgga gattttcctt cacgaacgac accatcccta cctttgcgct
    11461 gccggaactc cccgcgcgcg cgctggggaa aatcgccggc tatgtgcagt ggcgagagcg
    11521 ccccgccggc atggtgccgg acttcgacga tctggacctc ccgaccgtga cccgcatctg
    11581 ccatgacgcc atggccgcac gcggagacgg ctggctgacg acagccgaga cacgggccct
    11641 gttgagcgcg atgtcgatcc cgctgccgcc cggcggcgtc gcgaccgacg ctgaagaagc
    11701 tgccctgctt gccgaacaaa tcgggtttcc ggtcgccgtg aagctggcgt cccatacact
    11761 ggtccataaa acggagatcg gcggggtgca tctgaacctc aacacgaagg ctgaggttcg
    11821 ccgcgcgttt gaacagatcg ccgcacggct cgcgcaggat cagagccttg acgccatgga
    11881 aggggtcctg gtccagccga tggtgaccgg cggcgtcgag gtaatggcgg gcatggtcca
    11941 ggatccctcg ttcggtccct tgatcggatt cgggctgggc gggattcacg tcgaaattct
    12001 aggagacgtg cgcttccgca tcacgccgtt gacggagctg gatgccgccg atctgatccg
    12061 cagcatcaaa ggctatcgtc tgctgcaagg ctatcgcggc catccaccgg gcgatgtcga
    12121 tgcgattcaa gaactcttgc tcaggctgtc acggctggtc gaagaggtgc cggagatcgt
    12181 ggaactggat ctgaatccga tcttcgccat gccgccgggc cagggctgcc gcatcgtgga
    12241 tgcgaggatc agggtgggaa ggagtgttgc gagatggaat ggttaaacaa gtcggaaacg
    12301 atcagtattc caatttgagc acagcattaa tctactgagc cttgaccatt catctcggct
    12361 gaacgtttcg aaatcttcgc ctccacctca gcacgcgtca cccctaactg gtcaagcgcc
    12421 tcatacacaa ctctttcaag ctctggaaca cagttcctaa ataaggaagc tgccatttga
    12481 agttctcgaa tggcgtcggt tagcctcatg gcttcagaaa gcggtgaatc actaggccga
    12541 aagaatccgt gtacaattcg gttcctgacc tttctcgctt cggtaaggtt caaaagtaga
    12601 gtctcggata attgcacatt cttgtgggat tttacctctt tgaacaaatc cctcaatcgc
    12661 agcttgccga aaccatcata cagcgtgggc caatcatgat gcttgtcagt gcctcgcaga
    12721 gagagagcag taacaagaac ctgcaacaac atttctatcg tctgcgcttc agacaaagcg
    12781 aaaccaaacc aagcataaac atcccactcc tccttggaag cccattcagg atcgttgaac
    12841 actcgactta gcgactttta attcaggtaa ttttatgcgt tgccaaccaa tcagttggcg
    12901 ttgatatagt tgtcacccca aacgcctgcc ggatttctac gattcacgcc gcactccgat
    12961 taaagggacg gggccgtttg gatttcgggg cagctattcg tcgcgtcggc tccgcgtgca
    13021 acttcaaccc caacgctctc aagaccttga gaatcgtatc gaaacctgga ctccgctcgc
    13081 cggagagcgc cttgtacaga ctctcacgag acagacccgc atcctgcgca acctgcgcca
    13141 tgcccttggc gcgggcaata tcgcccagtg ccttggcaat gaaggccgcg tcgccatcgg
    13201 cctcctccaa gcaggcctcc aagtacgcag ccatctcctc cggagtccgc agatgctcgg
    13261 ctacgtcata tttggtagtt gtaaccttct tcatgatgca ctcctatagg ctccgtgcca
    13321 gccgcaaggc tgttcggatg tcggcatcct gtgtccgctt atcgccgccg gccagcagca
    13381 caatgacctc actgccacgc tttgtgaaat acacacgata gccggggcca tagtcgattc
    13441 gcatctcaga aacgccttcc cccaccgatt tcgcatcgcc cgcattccct gtaccgagcc
    13501 gctctattct aacctgaacg cgtgcacgtg cacgcatgtc acgaagtcca tcaagccact
    13561 tagcgaacgt cttggtcttc cggatctcga acctgcagaa ctgtatccaa ctggctacag
    13621 tctgtcaaca gtcctatccg gcgtcaatgt gtccgcctcc gttccagccg ccgcagcatg
    13681 cgcctcgcag ccaccggcac ctgcctcccg cacgcgcccc tcttcatccc acacccgacg
    13741 tctcgcaccg acatgcgtca gcagacattc cgccgtcgca agaagatcct gcacctggcg
    13801 agccgagcgg tcggcataat gcgccaggtc gaagatcggg atctggagac agcctcggct
    13861 cgattttttc gctccgggga gaagacgatg cgatgcgcgg gaggctgcgg atgctcgcag
    13921 ggcttcagaa gatccagcgt ctgaaagaac acccggcaga gccgttgcaa gtcgccaaga
    13981 atttcacgat gctcggcatc atcattgagc ggcaactggg tcggatcgcg aaacgccgca
    14041 tagaggttca gctggaatcc aatctggacc acattgccgg caccggtctt cacataaaac
    14101 gggtcatgat cgaaacagac ttttcgcatc acgatcatat cgcgcagagc ttgttcggtg
    14161 ggaaaggctt gttcgagatt caattgcatc gccactccat catactccct tcaggatgct
    14221 cccctgccat gacaggcaag gcactcggcg atacgaccag gctgatgcgc gccacgcccg
    14281 cgttcataca actgccgcac gatcggctca tctctcaacg tcggcttggt cgcgaccctc
    14341 gcgccgaact cgtgacaggc cgtgcaggaa ctggtgccgg ttgcacggaa gacaaattcc
    14401 ccgtgggggt tggtcctcgt gcgctcggta atttgagcaa gcgccccgcg atgttcggtg
    14461 tggcaggccg tgcaggccgt ccgttgcgcc accaggtcac ggtgccaggt cgtcacggca
    14521 ggcgtcgctc gatccgcgaa ataccgatcc gaatggcaac tcacacagcg ggccggactg
    14581 ggcccgcgga atggttcgtg gcagtgcagg cacccggcct gatcgacgtg ataccggctc
    14641 aactctcccg gcgcccagaa cgaggcggga cttctcaccg atccccagcc gatccagacg
    14701 aggagcacaa gcgtcgtgag agccaccacc ggcaccgcag ttcttggcag cggatggtat
    14761 cgcatccgtc acgagattcc cgcgaagaac aacgcccccg ccacgtgcaa cagcgtgaac
    14821 acgagaaaca tcaacgtcat cggcgcgtgg atcgcctgcc acatgcggaa cgcttcctct
    14881 tgagacgcca tgccatgcaa ctgcgcgtcg atatcgctgt cggacaatcc ttcgtgctgg
    14941 agtcggcggc gctgatcgtt gagcgcgctg aggctgaatg catgcaccgc ctgcccgacg
    15001 accccgctga cgaccacgat caccatggac agcatcgcga gaatcggtac cagggcgtga
    15061 tagtgggcgc cggagtggag aaatatgaac agcgggccca ccacccccgc caccatgtgg
    15121 acccgaaacc agccgcgcgg ccaacggcgg gccgggctga ttcgcttgcg caacggatac
    15181 acaaatacca gcagggtcac gacgaggccg acccggccgg ctgcatggcc atactgcgta
    15241 tgcccgaaag gggcggtctc tccgagactg agcagggcct cgaagatcag ggcgcccgcc
    15301 actgccgcca ccacgacgac agtcaccaca agaacacgca gccgaacaga cgtcatacct
    15361 cgctcgctca gaacaagagg agagccaata gcgtatggct tatagtaaat aactcaacac
    15421 aagccatcat cggccataag ccattcgctc cctgacctac ttccggtact tttccacacc
    15481 cttattgccg ccggcatggc accgcagaca gtctgcgaac cgtccgggac catgaacacc
    15541 cttggcctta gctttgagtt cctggaccaa tgcattgtcc tgtacttgcg gactcaccgt
    15601 cccctctttg cccgcccgat ggcaatccag gcacgtagcg gcgcccgtcg cccgaaagat
    15661 caaatcgctg tgcggattgg ccaccatgcc accggcatca gctatcgtcc acgcaatcga
    15721 cggcccctcc gcctccgacg taatcacagc accatacggc accgcatagg tccatcgcgg
    15781 cactgacgct acgaccagca ctcccaggat acacaacgca gcacccaggc ccgggcgact
    15841 catgagtgcc cgctcccgca acaggtccgg tatcgctctt cagggatgtc tttcatcgct
    15901 tgatacagct ctgcccgttc ttgatcgctg atcacctgat cgatcgacgg atagagaatc
    15961 cgttcctcct tcatgttgtg cgatttcaga atatccaaca accgctgctc gtcccgatcg
    16021 ctctccggat cctgcgcctg gaccttctga tgaatcgctt ccagacaatc gccgatcatc
    16081 cgatgctcgg ttcgcatgac ctgcgtcgga ccgccttccg ccataccgga gttctcctcc
    16141 catttgggaa acaggacgtc ttcctcccag acgatgtggc gttgcagacc gaacttgaag
    16201 gcgacgaacg cctctttggc cttggcgaaa tcctttcgtt tctgctcctg aaacgtggtg
    16261 aacagggcat cgagtcggtc gtgatcctgc tcgaacgtga cgctgatctt tccgtcggac
    16321 atgcggctct ccttggttat gaactacgca gtttgtactc caggtgacag gccacgcagg
    16381 ctttcaacac tttgttgaac tcgggcagga tctgcttcag atcatgcgac ggcatcgcat
    16441 cggccagctt ttccgcggct tcatgatgcg ccatcgccag gtcatggtag gccggcggaa
    16501 agttctccgg ctgctggcga gccatcgcgt gccggtgcat gaaaaagccc aagtgcgttt
    16561 cggtcaagcc ttgagcgagc cggtaatcct cgtcggccag cgcggcgacg atcgcttgaa
    16621 ccgtctccaa atgttgcagc atgacggcgc gatgctcctg tcgggcctcg gcatcgagct
    16681 ggaccggcgt gcgcacatcc ggcaaatgaa ccggcgtcac accattgatg ccggatgcag
    16741 ccttcggctc cgcgcagccg ctcacgagga gtgccgcgaa gagcagcagc ggaagagcgg
    16801 cttgtcgaat catgattcct ccatgattgc ccgttcccaa taggcgtcca cgcgcgcttg
    16861 aatgtgctga atcgcctccg tctgcatggt cggctcaaac aggtgccgga aacgcccctg
    16921 tagcttcaga taggcttcga caggcttgcg cttcggaacg taggtgtggg tgaccactcc
    16981 gtcgatcgcc tctttcaagg gccagagccc ggtctcgacc gccagcttcg cgacttccgg
    17041 tgtttccccg ggatcgtaga gccagccggt cgggcaggcg gctaacatga gaaacatctt
    17101 cggccccgtg aaggcagccg cccgcgcaaa tttttcttcc agatccagcg ggtatcgagg
    17161 cgcgacggtc gcgatgtacg gcggtttgtg cgcgcgccag atttcaaaga tgtctttctt
    17221 ctcttgagat gccccgctcg gatgctgcag gctgcaaggc gaggtggctg tccgcgcccc
    17281 ataaggggtc gccggggaca gctgcatacc ggtgttcccg tacgcctcgt tgtcgtaaca
    17341 gatgtaatag aaatcgagtc cgcgattcat cgcgcccgag gtggacgaca gcgccatatc
    17401 gtaggtcgag ccgtcgcctc ccagcaccac gactttcagg ttctcgcgct tcggtaaccg
    17461 acccttggcg atcaatacgt ccaacgcatc acgcacaccc tgcgcaccgg caggcgcgga
    17521 gcccatcgtc gtatagagcc atgagccctt gaatgacgta tagggatagg ccgccaacat
    17581 ggtgaagcaa ccggccgcgt tgacatacac gacctcgtcg ccgagcacct tcgccgcaag
    17641 tcgcaacgac tccaacccac cgcacccggc gcagagcgcg cttcccggaa gcacatgctc
    17701 ctcacctgga acctgcttga tcttcttaaa cgtctctcgt tgccacatgg cgtctctcgt
    17761 atctcgtgat tcgtatctcg ccgcgtccga aaggtcagca ggatttcttc ttcacgtttc
    17821 gcgtttcacg cttcctgccg ctaccatctg gagctgagtc atctcccgat gctcggcttg
    17881 ggtgtagagg agaaccggac cgattccttt tccgctgact ccggctttcg ccgtttgctc
    17941 gaagatcgcg tgaaacgccc ccctcgacaa attctggccc ccgagccctc cgatgaagga
    18001 acagagtgcc cgcggccgtt ccacttcgtg atacagcgaa gcggcaattt cctgaaacag
    18061 aatgccaccc tgccccggcg caagattctg atccaacacg gccacagcgc gacgcccccg
    18121 caatgcgtcg cggatggcat ccgccggcca gggccgaatc atccgaagcc gcaacaggcc
    18181 cacccgtttc ccctctcccc tggctgcatc gaccgccgcc ttgcccgtgc tcgaacagga
    18241 attcgtcatc acgagcacat cctccgcatc gtccagccga tatccctcga ccacatcgta
    18301 acgccgaccg aatacctgtt cgaaagacgc agcggcttca cggtgcacct cgagggcatt
    18361 gatcgaggcc agatgcattt gatgccggaa ataggcatag ggcgtcccgc cgatcaccgc
    18421 cacccctaac gaatggggcg cagacgcgct gaacccgagg tgagcgggac ggaacggcgg
    18481 cagaaacgtc tgcacttgct ccggatccgg gatacggacc ggctctcgcg tgaacgagag
    18541 cgaaaagccg tccagattca cgatgaccgg caacatcacc cgttcatctt ctgcgatccg
    18601 ataggccatc agaatcgagt cgagtacttc ctgacaggtc tcggcgtgta tctgcaaaaa
    18661 tccggcatca cgcgccgcca gcacatcgtt atgatccgac tccaacgtaa tgggcgcagc
    18721 cagtgcccgc gatacgttga ccagcacaag cggggcacgc cagcccgaga tcgagtagag
    18781 cacttcgaag gcgtagagca agccctggct ggatgtggcg gtgaagaccc gggcccccgt
    18841 ggcctctgcc gc
//
LOCUS       DAORRQ010000073        17203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_181_length_17203_cov_109.540588, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000073 DAORRQ010000000
VERSION     DAORRQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181_length_17203_cov_109.540588"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PK224_17350"
     CDS             <1..645
                     /locus_tag="PK224_17350"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRB17566.1"
                     /translation="DQCNAGGFQKPPFVNSLLDHCARGGAVATPGVVHRLDKDTSGVM
                     VVAKTEEAHRKLSVQFERHSITRHYEALVVGVPAKSEGRIEAALGPDRHHPKRTSTQT
                     LQPKRAVTDYRVEEAFGEAAARIVLSPHTGRTHQIRAHLQSINHPILGDQVYGGERVG
                     AIAGYEIPRVMLHARRLGFTHPVTGMYGEWTAATPSDFDRVRGVLCAGRLERKT"
     gene            complement(628..1113)
                     /locus_tag="PK224_17355"
     CDS             complement(628..1113)
                     /locus_tag="PK224_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17567.1"
                     /translation="MAADFIHRILRSLQGTFVPTPVAQPAIVDNRPEQPTPPPVRERR
                     IDTRFAVRTPCVYELVEGHGSNASTILGKAYSLNVSSDGILLLLDQKPQSRQLLNIRN
                     PALQRQRSLTLFEVRWSTHLPVGTTHPRYLVGCRLAFGRFPYFLVQRQHLDRYISGLS
                     L"
     gene            1497..2528
                     /locus_tag="PK224_17360"
     CDS             1497..2528
                     /locus_tag="PK224_17360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRB17568.1"
                     /translation="MNRISSTPVEIVVTGGESSKRIDVFLANRDPTFSRSALQRLIGE
                     GRILINGQTIRPSQKIKPGDRIRLEVPRPEPLDLKPEAIPFDILHEDADLLVLHKPAG
                     LVVHPAPGNWSGTLVNALLHHFATSGVTPSHIGGKERPGLVHRLDKETSGVMVIAKTD
                     QAHRALAAQFKLHTITRVYEALIWGVPKKGHGLIELAIGRDTKERKKFSARTTKPKPS
                     ATDYQVDERYGKIAAHVRLSPRTGRTHQLRVHLTSIDHPILGDKTYGGAKVMSVAGVP
                     IPRVMLHARTLGFTHPTGGEYHQFDVPCPPDMEQVQDLLRAATAHQESRAIGPSQIEE
                     HAEHDGRRE"
     gene            2542..2964
                     /locus_tag="PK224_17365"
     CDS             2542..2964
                     /locus_tag="PK224_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17569.1"
                     /translation="METSAVLDRIGELIAQLKGYASKLPPVVHLASVPTGVKPKPEAV
                     EVFEQALLRFREQSGRSPFKSLQDYLIESLEAFEVGNVLGVVQPLLAVLDQVERMHRD
                     REIKVGPAEEKRMGEYRAALVKILPGSQPELEGAGRGM"
     gene            complement(3023..3202)
                     /locus_tag="PK224_17370"
     CDS             complement(3023..3202)
                     /locus_tag="PK224_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17570.1"
                     /translation="MGTQVRSCPKCFQLMWLKQDHYDVLDEETVRAKCPHCGATVRFQ
                     LVTAGANAAGPKMGH"
     gene            3266..3709
                     /locus_tag="PK224_17375"
     CDS             3266..3709
                     /locus_tag="PK224_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB17571.1"
                     /translation="MPPALKTDQHPITFSDRNDFDAAQLIHLYRQAPWAKHRALEQAQ
                     AMLAKTDVVISAWDGTRLVGFGRVLTDYVFRASIWDVIVDREYQKRKIGTEIVRRILD
                     HPTLQHVELFWLCTRMPGFYERLGFSAKEQTGMVWSRKKSEPQAS"
     gene            complement(3815..4609)
                     /locus_tag="PK224_17380"
     CDS             complement(3815..4609)
                     /locus_tag="PK224_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRB17572.1"
                     /translation="MQRSLLAAALVGAVCSVIGVFVVLRGLAFAGAGTAHAAFAGVTL
                     AYLLGLPPLSLAIVFGLATVWITGWVEEKGRMKLDVSIGILYTATMALAILFLGLMKT
                     YNPEVYGYLFGSVLSVTTEELMTIGGLSVVVLGTILLLSKELYFIAFDQEMAAASGVP
                     ARQIFYLLLTLVALTVVIALKTVGAILVFAMILIPASTAYQLTHSLTQMTLYSMLIGI
                     FCAVSGVLLSYAFDLPSGPSIVLLATSLFFLAVCCSPKRLHRIQPT"
     gene            complement(4917..5696)
                     /locus_tag="PK224_17385"
     CDS             complement(4917..5696)
                     /locus_tag="PK224_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRB17573.1"
                     /translation="MTHPIIRFDHATFGFPGIVALEDISLTIPESEFVGVIGPNGSGK
                     TTLCRAVLGLMAPLSGTLRVLDCACEELRCHHRALIGYLPQKGMIDRNFPVTVLEAAM
                     MGRYGALGLFRRPSQKDRDIARQALAQVGMDHHRDSALGALSGGQQQRVFIARALAQQ
                     PRILLLDEPTTGLDLTAQHSVVELIQQLHHELKLTILMITHDINMIRSRVDRLVLLKT
                     KLFAEGPPQEVLKPEILSQVYGKELVITDKDFVLVEDYHHH"
     gene            complement(5757..6683)
                     /locus_tag="PK224_17390"
     CDS             complement(5757..6683)
                     /locus_tag="PK224_17390"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRB17574.1"
                     /translation="MFRLIALAMFLAFAMPCEWGSSATAFAASAAEPLRVVVTLPVLK
                     DWVQQIGGSYVHVNSLMTGYESEHTYSPKPSDLVAVRKAALLFEVGAGLEVWVASLVK
                     NAGNGSLRVVTTSKDIELIQDHPEPAGTSHSHEHAAGNPHVWLDPAAAATMVQHISDA
                     MAAADPAHATDYRTNTATYLQALTRVQDESLARLRSLPSRAIIVHHPAWPYFARRYDL
                     HIAGTIQTQPGGEPSARHLHTLIETIKRDHVRVIISEVQLNQKVPQLLARETGARIAV
                     LTTLPGGVPGTETYLDMLRYNVLQLAQALEQT"
     gene            6846..8030
                     /locus_tag="PK224_17395"
     CDS             6846..8030
                     /locus_tag="PK224_17395"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRB17575.1"
                     /translation="MKVVGLMSGTSADGVDAALVSIVRRGGTPRITPLAFASLPYPRS
                     LQQRIVELSLHGRVDDICHLNTYLGELFAKAALRVIQQSKCRPADIDLIGSHGQTIHH
                     LPKGRREPGVGLVRSTLQIGEPAVIAERTGITTVANFRPRDMAAGGEGAPLVPYAHAV
                     AFGHARRGRLVVNIGGISNVTYLPPGGDVADLRAFDTGPGNMVLDAIVHETSKGQSAY
                     DAGGRRARKGTVNRPLLTELMAHPFLTRRPPKSTGREEFGAPFVRSVLVKQRQARLSV
                     EDLLATCAAWTAEAIGSSRRWVTGEIHDVIVGGGGVYNRAIMASLRQVFAPTSVLTFD
                     ECGWSSKAFEATAFALLAHDTYHGQCTNVPQVTGAHHPVLLGAVVPGSPHMLTKWARL
                     AR"
     gene            8046..8564
                     /locus_tag="PK224_17400"
     CDS             8046..8564
                     /locus_tag="PK224_17400"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HRB17576.1"
                     /translation="MELLYPILGIGALVFLGSLAWESFGRRRSPGASTAEAPAGMSFK
                     AKPLLTDQEVQLYNLLRLAVEDRYLLFTQIPLWSLLDLRLPSGTPPAEMLRELALKRA
                     TFVLVHPGSRLVEKVVQVERPVPDDAPDGSHDSLFESALRAAGVQLVRLSPLHAYTVP
                     GLVTVLDLADPD"
     gene            complement(8615..9136)
                     /gene="ybgF"
                     /locus_tag="PK224_17405"
     CDS             complement(8615..9136)
                     /gene="ybgF"
                     /locus_tag="PK224_17405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRB17577.1"
                     /translation="MTRTHTKLGPVRGICLILALIGNLTTVPVLFAASPQPPDDAHRL
                     YDRVMEEFRHKDYAAALAGFRFFLELHGQSSLSANAQYWKGECQYRMGRYKDALDSFY
                     NLISDYPMSQKLAASTLKIGQIYTKQGDREKAQMMFERVTDQYPDSTEAEVARKALEA
                     AAAKGEPVAAEPS"
     gene            9464..10297
                     /locus_tag="PK224_17410"
     CDS             9464..10297
                     /locus_tag="PK224_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRB17578.1"
                     /translation="MERPQESGAVGGRREPLSEPPPNPKGGRTLIVDARDPHAYIRPS
                     AALKEAGETDQVFIRPGVYEDKVFVADRPVLLIGAGRDHVQIYSRRGGPLYLQRVPSG
                     MISGITFRYVGSDQHSAINVLDSVCTVTQCRATEGVLSGVVIYGPEARATFVENEVCY
                     NRESGIFVFAGAQPRVAQNQCYGNHHFGLAVRDPGSHPECVRNVCHSNMLSGILLFHH
                     AEALLLDNTCRDNQHWGLVMTPDTHPTPPREDLVTSNVLLPNPRGSLVVTEQPLADIG
                     R"
     gene            complement(10378..11310)
                     /locus_tag="PK224_17415"
     CDS             complement(10378..11310)
                     /locus_tag="PK224_17415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HRB17579.1"
                     /translation="MIKLLLVEDNPVDAQLTRDLLAEWPLDQFEITHAPILADGLTRL
                     SRDRFDVVLLDLSLPDTHGLSTVTQVLATSPGVPVVVLSGHDDHPLALKALQHGAQDY
                     LVKGEGGTDFLARSILYAIERKRAQERLTYLAQHDQLTGLINRALFRDRLIHAMARSK
                     RKDHPLAIMLLDLDRFKAVNDTLGHDVGDQLLKLVATRLLECVREVDTVARMGGDEFT
                     AILEGISGVADVLVVAKRIVESISTPFEIGPHRISIGVSIGITLYPLDDEDIDELLRH
                     ADKAMYAAKQQGGSRFHFHSTTGQPPSGTPAPVQ"
     gene            complement(11933..12901)
                     /locus_tag="PK224_17420"
     CDS             complement(11933..12901)
                     /locus_tag="PK224_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HRB17580.1"
                     /translation="MRLITTKELRTLHIATRHHHLINSRLAILRYADEYGFKGAARRF
                     GLDRKTVRTWHRRWVASGPAGLVPRHPVKRRRRLSEETVRLIEQARRELHFGAMRTRF
                     WLDRVHHIRVAAATIRRICRDLGYPPIRRTDPRRPRHPILFSKEHPGECVQVDVKEVK
                     VAGQKYFQYTALDDCTRYRVLRLYPRKYHGTSLEFLATIRQVFPFPIRKVQVDNGTEF
                     PLAFALAVQEAGIRLRHIKPRRPEQNGKVERSHRIDEEEFWSRSSFDGFASATQALRT
                     WEHQYNHDRFSMALQGLTPAEKLATFLSPLHPSSPNTPCTHSGAAA"
     gene            13000..13194
                     /locus_tag="PK224_17425"
     CDS             13000..13194
                     /locus_tag="PK224_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17581.1"
                     /translation="MDHEKAVRQELRVVSHNLGGQSDDGGRVTATWAIPLALAGVLVA
                     GEPVSADSEIYGYVGPKGVF"
     gene            13195..13773
                     /locus_tag="PK224_17430"
     CDS             13195..13773
                     /locus_tag="PK224_17430"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRB17582.1"
                     /translation="MTNVPTDQRFRSAESQVRRLSHRASAEEVEEAVKRYAFQFQLHP
                     ALLLAVIKAESDFNPTVISRSGAVGLMQLIPETAIRHGVLNLYDTGDNIRGGARHLRY
                     LLDRFNGNVRLAVAAYNAGERRVERYRAIPPYPETRDYVRKVMIYYKSFRGDYQASPG
                     KAVLLAMYHKPLQLEPLPVSTDVRNSRPAVRK"
     gene            14451..15563
                     /locus_tag="PK224_17435"
     CDS             14451..15563
                     /locus_tag="PK224_17435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB17583.1"
                     /translation="MISVFGSCVGQEEIDEIRTSFEKQWLGIGLKTRSFESLFAHRLG
                     LKDFVFLDNGSNSLYMAVKLLNLSPGSEVILPSFTWVACAHAIILAGGIPVFCDVDVN
                     TQNVTADTIASHISSKTRAIMVVHYAGKPVRMEPVLSFGQPVIEDAAQAVDSKLGNKY
                     CGSIGTIGVYSFDPVKNITTGEGGGITTRDSELMTRARQLRYCGIGSAGFEAAAMKER
                     WWEYDIVDVFPKLLPNDIAASIGLAQLRKIDKFQTIRKKIWNIYQAQLADFDWLIRPQ
                     DPASDEQHSYFTYSIRVAKGKRDQLAKYLYSRGIYTTVRFHPLHLNPIYKSSYRLPIC
                     EQLNDEILSLPLHPNLSDSDIDAILLSLQKFNEQYM"
     gene            15895..16788
                     /locus_tag="PK224_17440"
     CDS             15895..16788
                     /locus_tag="PK224_17440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB17584.1"
                     /translation="MPRVSVIIPAYNSMQWVSAAIDSVLNQGYRDYEVVVIDDGSTDQ
                     TADVLESYGRKIRCIYQANAGVSNARNRGLSVSTGEFIAYLDADDMWYSQKLECQIAF
                     LDAHPQCGLVHSDVTVIDEQEKVIRARFNVETARPVPSGYCLQDLLRRCHIQVPSVIE
                     RRECIEEVGGFDEQFMATEDYLHWIMVSLKGWEFGYINEPLAKYRWRGGSVSSNKRLF
                     LEEYGKMFGVLLPALYENRPQAPDLVIIRKRFLQVERELAYMDCIEGHLSNARQRLMR
                     LIQRAPFQYNLYWDLAKTYLR"
BASE COUNT         3581 a         4668 c         5293 g         3661 t
ORIGIN      
        1 gatcagtgca acgccggtgg gtttcaaaaa ccaccgtttg tcaacagcct gctagaccat
       61 tgcgcccgcg gaggtgcggt ggcgacgccc ggcgtggtgc atcgtttgga taaagataca
      121 tctggcgtga tggtggtggc caagacagag gaggcgcatc gaaaactgtc ggtgcagttc
      181 gaacgacatt ccattacccg tcactatgaa gcgctcgtcg tgggcgtgcc ggctaaatcg
      241 gaaggccgga tcgaggctgc gcttggtccg gatcggcacc atcccaaacg cacctcaacc
      301 cagacgcttc agccgaagcg tgcggtcaca gactatcgag tcgaagaagc ctttggcgaa
      361 gcggcggcgc gcatcgtgct cagtcctcac acaggccgta cgcaccagat acgggcccat
      421 cttcaaagca tcaaccatcc catcctcggg gaccaggtct atgggggtga gcgggtcggt
      481 gcgatagcag ggtatgagat tccccgagtg atgctacatg cgcgtcggtt ggggttcaca
      541 catcctgtca ccgggatgta tggcgagtgg acggctgcca cgccgtccga ttttgacagg
      601 gtacgaggag tgctatgcgc cgggcgatta gagcgaaaga cctgagatgt agcggtccaa
      661 gtgttggcgc tggacgagga agtaggggaa gcggccgaat gcgaggcggc aaccgaccag
      721 gtagcgcggg tgggtggtgc ccaccgggag gtgagtggac cagcgcacct cgaacaatgt
      781 cagtgagcgc tgtcgttgca gtgccggatt ccgaatgttg agtaattgtc tgctctgagg
      841 tttctggtca agcaagagga gaatgccgtc cgaactgaca ttcaacgaat aggccttccc
      901 gagtattgtg gaggcgttgg agccatgtcc ctccaccagt tcgtagacgc aaggagtcct
      961 gacggcaaac cgagtgtcga tgcgccgttc gcgcacgggg ggtggcgtcg gttgctcagg
     1021 ccggttgtcc acaatggcgg gctgagcgac cggtgttgga acgaacgtgc cttgcagcga
     1081 cctgaggatt ctgtggataa aatcagcagc cattggatcg tcacaatctc cagctgttga
     1141 agcgccaaac cttcttaata aataagcaag tcaggtgcct gtagcgggag agacaggcat
     1201 catgctaaca acatgaattt tttctggagt gcgagtgtgc catcatgcga catcagcgga
     1261 atgcgcagta tcatggtgtc aaatccaacg caccactgac agaaatgaac aggcaaacgt
     1321 acattcgtgc cggcgctgaa ggatgcgcgt gtgccgcgcc acatgtggat ggattgtgga
     1381 cgacatgtgt gtgaggtgtg cagaggatgg ggatggagcg gcgggtacct tgtgtttgac
     1441 cggcctctgt ggcagcctat gcccacgcat gtcgcaggat gtcaggcagt caccacgtga
     1501 ataggatcag tagcactccg gttgaaattg tcgtgaccgg aggagaatcg tccaagcgga
     1561 tcgatgtctt ccttgccaac cgcgacccga ccttctctcg ttcggccttg cagcgcttga
     1621 tcggagaagg gcgcattctc atcaatggcc agacgatccg gccaagtcag aagatcaaac
     1681 cgggtgaccg catccggctg gaggtgccgc ggcctgaacc gctggatctc aaaccggagg
     1741 ccatcccctt tgatattctg cacgaagatg cggacttact cgtgctccat aagccggccg
     1801 gcctcgtggt gcacccggcg ccggggaatt ggtccgggac gttagtgaac gccctcttgc
     1861 atcactttgc gacctcgggt gtcaccccgt cgcatatcgg aggtaaggaa cgcccaggac
     1921 tcgttcaccg attggacaaa gaaacctcgg gcgtcatggt gattgcgaaa acggaccagg
     1981 cgcatcgcgc gctggccgcg cagttcaagc tccacacgat caccagggtg tacgaagcct
     2041 tgatttgggg cgtacccaaa aagggccatg gcctcattga actggcgatc gggcgcgata
     2101 ccaaagagcg gaaaaagttt tcggcgcgga cgaccaaacc gaaaccttcg gcgaccgact
     2161 atcaggttga tgagcggtac ggcaagatcg cggcccatgt gcgcctctcc cccagaaccg
     2221 gacgcaccca tcagctgcgg gtccatctca cgtcgatcga tcatcccatc ctgggggata
     2281 agacatacgg cggggccaaa gtgatgtcgg tggcgggggt gccgattccc cgtgtcatgc
     2341 tccacgcgcg tacgctggga tttacgcatc ccaccggcgg ggagtaccat cagttcgatg
     2401 tgccctgtcc gcctgatatg gagcaggtgc aggatctcct tcgcgcagcc acggcgcatc
     2461 aggagtcccg ggcgatcggc ccgagccaga ttgaagaaca tgccgaacat gacggtcggc
     2521 gagagtgagg agcgacgaac gatggaaacc agtgccgtgc ttgatcggat cggtgagttg
     2581 atcgcgcagc tgaaagggta cgcctcaaaa ttgccgccgg tggtacatct cgcgtcggtg
     2641 ccgaccgggg ttaaaccgaa acctgaggcg gtggaagtgt tcgagcaggc ccttctgcgg
     2701 tttcgggagc aaagcggccg ctctccgttt aagagtctgc aggattatct gatcgagtcg
     2761 ctcgaagcgt tcgaggtcgg caatgtgctg ggcgtggttc aaccgcttct ggccgtcctt
     2821 gatcaggtgg agcgcatgca tcgggacagg gagatcaaag tcggtccagc cgaggagaag
     2881 cggatggggg agtatcgcgc ggcgctggtt aagatcctgc cgggcagtca gccggagttg
     2941 gagggtgcag gacggggcat gtgacgctcg gagcggtcat gttcactgaa gcaggatcat
     3001 ccgttgaaca tgccgggaac ggtcaatgtc ccatcttcgg gccggccgcg ttggcgccgg
     3061 cggtgaccag ctggaagcgg acggtcgccc cacagtgggg acatttcgca cggaccgtct
     3121 cctcgtcgag gacgtcataa tgatcctgct tcagccacat taattgaaaa cacttcggac
     3181 agcttcgcac ttgcgttccc atggtcttcc ccttacacta cggttaaccg actcaccccg
     3241 ttcggaggga ttaatttagt acaagatgcc tcccgctctc aagaccgatc agcatcccat
     3301 cacattctcc gaccgcaacg atttcgacgc tgcacaactg attcatttgt accggcaggc
     3361 tccctgggcc aagcatcgtg cgcttgaaca ggcgcaggcg atgctggcga agacggatgt
     3421 agtcatttcg gcctgggatg gcactcgcct ggtgggcttc ggccgcgtgc tgaccgacta
     3481 cgtattccgg gcgtcgattt gggacgtgat cgtcgatcgc gagtatcaga aacgaaagat
     3541 cggcaccgag atcgttcgac ggattctcga ccatcccacc cttcagcacg ttgaactgtt
     3601 ttggctctgc acgcgcatgc ccgggtttta tgaacggctc gggttcagcg cgaaagagca
     3661 gaccggcatg gtctggtctc ggaagaagtc tgagccgcaa gcttcatagt ccatccgcct
     3721 ccaacggttt tcttttccgt actctccagt ccctgtcgac gatccctggt tcgcctatcc
     3781 ttgcctgtga ggggaacggt cctgccgggt tgttctatgt cggctgaatt cgatgcaacc
     3841 gtttgggcga acagcagacc gcgagaaaga aaaggctggt ggccaagagg acaatggacg
     3901 gaccggaggg aagatcaaag gcataggaga gcagtacgcc gctcacggcg cagaagatgc
     3961 cgatgagcat ggagtagagc gtcatctgag tcaggctgtg cgtaagctgg tatgcggtcg
     4021 atgcggggat cagaatcatc gcaaacacga gaatcgcccc gacggttttc agcgcaatca
     4081 ccacggtgag cgccacgagt gtcagcagga ggtagaagat ttgccgcgcg gggacgccgg
     4141 acgctgccgc catctcctgg tcgaaggcga tgaagtacag ttccttcgag agcagaagga
     4201 tagttcccag caccaccacg ctgagcccgc cgatggtcat gagttcttcc gtggttacgg
     4261 aaagcacgct gccgaacagg tacccgtaga cctcggggtt atacgtcttc atgagtccga
     4321 ggaaaagaat ggccagcgcc atggtggcgg tgtagaggat gccgatggat acgtccagtt
     4381 tcatgcgccc cttctcctcg acccaacccg tgatccagac cgtcgccagt ccaaacacaa
     4441 tggcgagact gagcggcgga aggccgagga gataggcgag ggtgacaccg gcaaatgcag
     4501 catgggcggt cccggcccct gcgaacgcga ggccccgtaa gacgacgaac acgccgatga
     4561 cggagcagac cgcgccgacc agtgcggcgg ccagaagtga gcgttgcatg aaatcgtacg
     4621 cgagcagttc gagcatcgta atatgtggtg accgaataat ctcgtgaatg aatgagtgcg
     4681 atgactgagt ctagcccgga ctattcatga atacctgctc cggcgtccga tcctctcgcc
     4741 cggcgaggct gttctcgttc ggcctcctcg acggactgcc agtacgcctg cgtcggcctc
     4801 acttgcgaga agccccggcg ggcttccgtc tcgaacgcct cgcgacggta ttcgtgaata
     4861 atccgggctc ggccctcgaa tcaggaccgt cgccagatcg attcatcgta tgtgcttcag
     4921 tggtgatgat aatcctcgac gagaacgaag tccttgtccg taataaccag ttctttcccg
     4981 tatacctgac tcagaatttc cggcttcaga acctcttgcg gcggcccttc ggcaaagagc
     5041 ttcgtcttga gcaacaccaa tcggtccacc cgcgaccgga tcatgttgat gtcgtgagtg
     5101 atcatgagaa tggtcaactt cagctcgtga tgtagctgct ggatcaattc caccacactg
     5161 tgctgcgcgg tgaggtccaa cccggtcgtc ggctcgtcta gcaagagaat ccggggctgc
     5221 tgcgccagcg cccgtgcgat gaacacacgt tgctgctgtc cgccggaaag ggcacctaag
     5281 gcggagtctc tgtgatgatc catgccgacc tgggccagcg cctgacgggc gatgtcccga
     5341 tccttttgtg acggtcgccg gaagaggccg agcgcgccgt atcgtcccat catggccgct
     5401 tccagtacag tcacgggaaa gttccggtcg atcatgcctt tttgcggcag ataaccgatg
     5461 agcgcccgat ggtgacagcg gagttcttcg caggcacagt cgaggacacg gagggttccg
     5521 ctcagcgggg ccatcaggcc gagcacggca cgacagagtg tcgttttccc agagccgttg
     5581 gggccgatca ccccgacaaa ttccgattcc ggaatggtca gggagatgtc ctccagggcc
     5641 acgataccgg gaaaaccgaa ggtggcatgg tcgaaacgaa taatgggatg ggtcatgcgc
     5701 ggcggtgtac gttgaaaatg ctggtttgtc ggtcgatgtc cgggtggctg cggcggtcag
     5761 gtttgctcaa gcgcctgggc caattggagc acattatagc ggagcatgtc gaggtaggtc
     5821 tcggtccccg gtacgcctcc gggcaaggtc gtcaacacgg cgatgcgggc accggtttcc
     5881 cgcgccagca actgtggaac cttctgattc agttgtacct cggagataat cacgcggacg
     5941 tggtctcgtt tgatggtttc gatcaatgtg tgtagatgcc gcgcggacgg ttctcccccc
     6001 ggttgtgtct gaatcgtgcc ggcgatgtgc agatcgtagc gtcgggcaaa atagggccag
     6061 gccgggtggt ggacgattat cgcgcggctc ggcaacgagc ggagccgtgc cagcgactcg
     6121 tcctgcacgc gcgtgagtgc ctggaggtat gtcgcggtgt tggtccgata gtcggttgca
     6181 tgggccgggt ccgcggcggc catggcatcg gaaatgtgtt ggaccatcgt ggctgcggcc
     6241 gccggatcca gccagacatg cggattaccc gccgcgtgct cgtgcgaatg gctcgtccct
     6301 gccggctcgg ggtgatcctg gatcagctcg atgtctttgg aggtcgtcac gacacgcaac
     6361 gagccattgc cggcgttctt caccagtgag gccacccaga cttcgagtcc ggcaccgact
     6421 tcgaacaaga gcgcggcttt gcgaaccgcg acaagatcgc ttggtttcgg cgaataggta
     6481 tgttcactct catacccggt catgagcgag ttcacatgca catacgaacc gccgatttgt
     6541 tgcacccagt ccttcaggac cgggagcgtc acaacaacac gaagcggttc ggcagcggat
     6601 gcagcaaaag ctgtagcgga ggagccccat tcacagggca tcgcaaatgc gagaaacatg
     6661 gccaaggcga tcaggcgaaa catccggcgg acggtaacac ccgatttctg caaagtcaac
     6721 gcgaaggccg gtggagattg tgcgcggatg atacgcgcga tgcttgaccc gttgccgggg
     6781 tttcgattag cgtagcgaac cgaacaggaa gaaatctccc ggttgtgtgc cggagaaggg
     6841 gcgcgatgaa agttgtcgga ctcatgtccg ggacatcggc ggatggtgtg gacgcggcgc
     6901 tggtcagcat cgtccggcgc ggcggaacgc cgcggatcac ccccctggca tttgcctccc
     6961 tgccgtaccc gcgctcgctc cagcaacgaa ttgttgaact ctcgctccat ggccgcgtgg
     7021 acgacatctg ccacctgaac acgtatctcg gggaactgtt cgccaaggcg gctctgcggg
     7081 tcattcagca atcgaaatgc cgcccggccg atattgattt gatcggatcg cacggccaaa
     7141 cgattcacca tttgccgaag gggcgacgtg agcctggtgt cgggttggtc cggtccaccc
     7201 tgcagatcgg tgagccggct gtgatcgcgg aacgcacagg catcaccacc gtcgcgaatt
     7261 tcaggccgcg cgatatggcg gcggggggcg agggcgctcc gctggtcccc tatgcccatg
     7321 cggtggcctt tggccatgcg cgtcggggtc gtctggtggt gaatatcggc gggatcagta
     7381 acgtgaccta tctgccgccg ggcggtgatg tcgccgacct ccgcgcgttc gacaccggcc
     7441 ccgggaatat ggtgctggat gcgattgtgc acgaaacgtc gaaggggcag agtgcctacg
     7501 acgccggcgg gcggcgcgca cgtaagggga cagtgaaccg gcctctgctc accgagttga
     7561 tggcccatcc gttcctgacg cggcgtccgc ccaagtcgac gggtcgggaa gagttcggtg
     7621 cgccctttgt tcgaagtgtt ctcgtgaaac aacgtcaggc gcgtctgtcg gtcgaggatc
     7681 tgctggcgac ctgtgcggcc tggacggcgg aggccatcgg ttcgtcccga cggtgggtca
     7741 ccggcgagat ccatgatgtg atcgtcggag ggggcggtgt gtacaatcgc gccattatgg
     7801 cctctctccg gcaggtattt gcgccgacat ctgtgttgac gtttgacgaa tgtggatgga
     7861 gcagcaaggc ctttgaagcg acggcgtttg ccctgctggc tcacgacacc tatcacggac
     7921 aatgcaccaa cgtgccgcag gtgacgggtg cccatcaccc cgtgctcctt ggcgctgtgg
     7981 ttccgggatc ccctcacatg ctgacgaaat gggcgcgtct cgcccgctga tccgattggc
     8041 ttgtcatgga attgctgtat cctatccttg gtatcggggc gctggtgttt ctgggaagcc
     8101 tggcctggga gagtttcggg cggcgccgtt cacctggcgc gtccacggca gaggctcctg
     8161 ccggtatgag ttttaaggcc aagccgttgc tgaccgacca ggaagtgcag ctctacaatc
     8221 tgctgcgcct cgccgtggaa gaccggtacc tgctgttcac gcagattcct ctttggagct
     8281 tgttggattt gcgtcttccc tcgggaacgc cgcctgccga gatgttgcgg gaactggcgc
     8341 tcaaacgcgc caccttcgtg ctggtgcatc cgggcagccg tttggtcgag aaggtggtac
     8401 aagttgagcg gccggtgccg gatgatgccc cggacgggtc gcacgattca ctctttgagt
     8461 cggcgcttcg tgcggcaggg gtgcaactcg tgcggctgag cccgctgcat gcctatacgg
     8521 ttcccggtct cgttaccgtg ttggatcttg ccgatccgga ctaacgccgg ttcgactgtc
     8581 aacgggcctg tgccgaaagg gcgcgcaatc cggatcagct tggctcggca gcgaccggtt
     8641 caccctttgc ggcagcggcc tccaaggctt tgcgagccac ctcggcctct gtgctatcgg
     8701 ggtactggtc cgtcacccgc tcgaacatca tctgcgcttt ttcgcggtcg ccttgcttcg
     8761 tgtagatctg cccgatcttg agcgtcgagg cggcgagctt ctggctcatc gggtaatcgg
     8821 agatgaggtt gtagaacgag tccagcgcgt ctttgtagcg ccccatacgg tactggcact
     8881 cgcctttcca gtattgagcg ttggcggaca gggacgattg cccgtggagt tcgaggaaga
     8941 agcgaaaccc ggcgagggct gcggcatagt ccttgtggcg gaactcttcc atcacgcgat
     9001 cgtagaggcg gtgtgcgtca tccggcggtt gaggtgaagc tgcgaacaga actggaacgg
     9061 tcgtgaggtt gccgatcaac gccaagatga gacagatgcc tcgcacggga ccgagcttgg
     9121 tatgagtcct ggtcatgagt gcccctcttt cttccctgtt ggacgagtct gggttcacac
     9181 ggtcgttgtg ctttccgata tgaaagaacc gcaccagcga ggctcgacaa gtgaacccgt
     9241 tgtgaaattg tatagttcgc gtggaattga aggcggagtg gcctggatgt gccggaacaa
     9301 caggggcgga aagcagacag tattgtacgg aaagcgccag ccatgaggcg ctgctctggc
     9361 cggttgacag atcgcatgtt ttcacttgtt gtcggttagc aggcagccgg ccatggcggc
     9421 gggtgtgctg attccgagtc tgtagaggat cgagaaagtc ggtatggaaa gaccacagga
     9481 gtcaggcgcg gtcggtggcc gaagggagcc gctcagcgag ccacctccca atccgaaggg
     9541 gggccggacg ttgattgtcg acgcccgcga tccccacgcc tacatcaggc cgagcgcggc
     9601 actgaaagag gccggtgaga ctgatcaggt cttcattcga ccgggagtgt atgaagacaa
     9661 agtctttgtc gcggaccgtc ctgtgctcct catcggcgcc gggcgtgatc acgtgcagat
     9721 ttacagccgt cgcggagggc cgctctatct ccagcgggtc ccctcgggca tgatcagcgg
     9781 gattaccttc cgctatgtgg gcagtgatca acattccgcg atcaacgtcc tggactcagt
     9841 ctgcacggtt acgcaatgcc gggcgacgga aggcgtgttg tcaggagtgg tgatttatgg
     9901 tcctgaggcc cgtgcgacgt ttgtcgagaa tgaggtctgt tacaaccgcg agtcaggcat
     9961 ttttgttttt gcgggcgcac aaccgcgtgt ggcgcaaaac cagtgttatg ggaatcatca
    10021 tttcggcttg gccgtgcgag atcccggcag ccatccggaa tgtgtgcgca acgtctgtca
    10081 cagcaacatg ctcagcggga tcctcctgtt ccaccatgcg gaggcgctcc tgctcgacaa
    10141 cacctgtcgg gacaaccagc actggggttt ggtcatgacc ccggatacgc atccgactcc
    10201 gcctcgcgag gacttggtca cctctaatgt gttgctgccg aatccccgag ggtcgttggt
    10261 cgtgacagag cagccgctcg ccgatatcgg tcgctaagca gtcgtgtcgt gcggaggggg
    10321 tgcttgttgc cgcggtcgat ctgtgaagaa agaacgggct cctgaagcgg cttctcgcta
    10381 ctgtaccggg gcgggggtac cggagggagg ttgtccggtc gtagaatgaa agtgaaatcg
    10441 gctcccgcct tgctgcttgg cggcatacat ggccttatcg gcatgtctga gaagttcgtc
    10501 gatatcctca tcgtctaacg ggtagagcgt gatgccgatg ctcacgccga tcgagatccg
    10561 gtgcggtccg atctcgaacg gggtgctgat cgactccacg attcttttgg ccaccacgag
    10621 tacatcggcg acgccagaaa taccttccag gatggcggtg aactcatcgc cgcccatgcg
    10681 cgcgacggta tccacttcac gaacgcactc cagcagtcgg gtcgccacga gtttcagcaa
    10741 ctgatcgccg acatcgtgcc ccagcgtatc attgacggct ttgaatcgat ccaggtcgag
    10801 cagcatgatc gccagggggt gatctttccg tttgctgcgc gccatggcat ggattaagcg
    10861 gtcgcggaag agggcgcggt tgatcaggcc ggtaagctga tcgtgctggg caagataggt
    10921 caggcgttcc tgtgcccgct tgcgttcgat ggcatagaga atcgagcgtg caagaaagtc
    10981 ggttccgcct tctcccttca ccagataatc ctgcgccccg tgttgcagcg ctttgagggc
    11041 cagcgggtga tcgtcgtgcc cgctcagcac caccaccggc acgcctggac tggtcgctaa
    11101 gacctgggtc acggtactca agccatgggt gtcgggaagg gagagatcga ggagtaccac
    11161 atcaaaccgg tcccggctta atctggtcaa gccgtccgcc agaatggggg catgggtgat
    11221 ctcgaactgg tcgagcggcc attcggccag gaggtctcgg gtgagctggg catcgacggg
    11281 attgtcttcg accaacagca gtttgatcat cggtgcgtgc tcgcgtccgt aagacaatct
    11341 ggcgaggcga tgtgctgcgc acaagcggat ggctgacaga tcaccgtgat gctgcgcgcc
    11401 ggaactatcg agtgtggaat tttcatctca aatggtggtg acggccgagg ggcgtattca
    11461 gtgcgggaat ctccctgtgg ctgcaggcag tgcgtgattg agggaccagc ccgaaatcga
    11521 ttctagcacg tcactctgaa gcttcaaaca agagcgcact tcttcacgat gatcgtgcgt
    11581 gatctgccgg catgccctgc gtcctggtgt cgaccgacaa tgcctcatgt gaagaaacct
    11641 cctcagttcg ggtctacaga tgcatgtcct gcccgcaaac ctgcgatggc gcgtcatgtt
    11701 ggtaacgaac agcaacgaaa caaagaacaa aatccggcca aatggtggca caaaacggtt
    11761 cggttcctct ccccgctcac caggggagag actccttgtg aacgccctaa ccatacgaag
    11821 ttattggagc tgcccgcttt tgtacacgtc aggacgatgg catgaaagct gcgcttcagc
    11881 ctggggttcg cgacgctgtg gattccccgg ctatgggttg tgtaatggct tgtcaagcag
    11941 cggcccccga atgtgtgcat ggcgtgttcg gtgatgatgg gtgcagtggc gaaagaaacg
    12001 tcgccaattt ctccgcaggc gtcaggccct gcaaggccat ggagaagcgg tcgtggttgt
    12061 actgatgctc ccaagtacgt agcgcctggg tcgcggacgc gaacccgtcg aacgaggagc
    12121 gactccaaaa ttcctcctcg tcgatccgat gactgcgttc caccttccca ttctgttcgg
    12181 gccgccgcgg cttgatatgc cggagacgga tgccagcttc ttgcacggcg agggcgaagg
    12241 ccagcgggaa ttctgtgcca ttgtccactt gtaccttgcg aatgggaaac gggaacacct
    12301 gacggatcgt ggccagaaac tcgagactgg tgccgtgata tttgcgggga tagaggcgca
    12361 agacccgata ccgggtacag tcatcgaggg ccgtgtactg aaaatatttc tggccggcga
    12421 ctttcacctc cttgacatcg acctgcacac actcgccggg gtgctctttg ctgaagagaa
    12481 tggggtggcg tggacgacgc ggatcggtgc gccgaatggg aggatagccg aggtcccggc
    12541 agatccggcg gatggtggcg gccgcgacgc gaatgtgatg cacccggtcc agccagaacc
    12601 gggtccgcat cgccccaaag tgcagctcgc ggcgggcttg ctcgatgagg cgtacggtct
    12661 cctcggacag ccgtcgacgg cgcttcacgg gatggcgcgg caccaatccg gcgggcccgc
    12721 tggcgaccca acgacggtgc caggttctca ccgtcttgcg atccaggccg aagcgtcggg
    12781 cggcgccttt gaacccatat tcgtccgcgt agcgcaaaat cgccagtcgg ctgttgatga
    12841 ggtgatgatg tcgggtggca atgtgcaatg ttctcagttc cttcgtcgtg atcaatcgca
    12901 tgggatcccc cctggtgata gctcgtgact gtaagccatg cggggggcca actctcaaca
    12961 agatatttca ggttgctgcg ggaacaagag gaggaagaga tggatcacga aaaagctgtc
    13021 cggcaggagt tgcgagtagt gagccataac cttggcggcc agagtgacga tggcgggcgt
    13081 gtgactgcca cgtgggcgat tcctctggcg cttgcggggg tgctggtggc cggggagccc
    13141 gtctctgcag acagcgagat ctatggctat gtggggccga agggggtgtt ctaaatgacg
    13201 aatgttccga ccgaccaacg gtttcgctcg gcggaatcgc aggtgcgccg cctgtcgcat
    13261 cgcgcctcgg cggaggaagt ggaggaagcc gtcaagcgct acgccttcca gtttcaactt
    13321 catccggcct tactgctggc ggtgatcaag gcggagtcgg actttaatcc caccgtcatt
    13381 tcacgatccg gggcggttgg actgatgcag ctgatacccg aaaccgccat caggcacggg
    13441 gtgctcaatc tctatgacac cggcgacaac attcgaggcg gcgcgcggca cctgcggtac
    13501 ttactggatc gatttaacgg gaacgtgcgg ttggcggtcg ctgcgtataa tgcaggtgaa
    13561 cggcgcgtgg agcggtatcg cgcaattccg ccctatccgg aaactcgcga ctatgtcagg
    13621 aaggtgatga tctattacaa gagcttccgt ggtgactacc aggcgagccc cgggaaggcc
    13681 gtgttgctcg ctatgtatca caagccgctg caacttgagc cactgcctgt ctctactgat
    13741 gtgcgaaact cacgacctgc ggtcaggaaa taggtggggc cgagtatctc atcttctcta
    13801 acgttcccag acgaggcgga caaataacca ggccgaccat tcggtcggcc gatacatcac
    13861 atcctcaaga tagggttacg aggcgtagcg cacatttagc agaccgaatg cgtggcgttg
    13921 gtcaacagcc accagtgctg ggtggccacg accagcgtgg ctctcttgcc tgttcaatct
    13981 tcaacactcc cggccttctt tgccgtgcaa gcttgggtgg tttcatcagg gtaatagggc
    14041 gcccttcatt cggcaaatgc tgcgcgctcg gtcacgctca accgtaacca tgtgcgaccg
    14101 catcatcgca caagtccgtc atcacatggg ccatgaagta atgaactggt tcggctatga
    14161 tgcgtcacat gtggtgacgg ttttgatagg gcaaacgagg ggcaagggag agctttactg
    14221 aagtcgcaca tcgccgaaat aaacgacggt ttgtgctcga tagcaatgca aatcgcggcg
    14281 gacgagactg aataggggca cggtgatcta gccatagcac ttcgcgacat gattgatgcc
    14341 gtgaaggtga gaattgtgcg gcggggtcac gttattaccg actgacactg taatttccag
    14401 ccgccgcggc tcatccgatc ctcagtatgt ccttgaatag gggccttact atgatctcag
    14461 tattcggttc atgcgtagga caagaggaaa ttgacgagat acgcaccagc tttgaaaagc
    14521 aatggctcgg aatcggtctg aaaacaagat catttgaatc tctcttcgct cacaggttgg
    14581 ggctcaagga tttcgtgttt ctggacaacg gttccaatag tttatacatg gctgtaaaac
    14641 tattaaacct atcccctgga tcggaggtca tactgccctc ttttacatgg gtcgcatgcg
    14701 cccacgctat catcctcgct ggaggtattc ccgtattttg cgatgtagac gtgaatactc
    14761 aaaacgtgac tgctgacaca attgcctcgc atatttcctc aaagaccagg gctattatgg
    14821 ttgtacatta tgcaggcaaa ccggtccgta tggagccagt gttatcgttt ggacaaccag
    14881 tgatagaaga tgcggctcaa gccgtagatt ccaaactagg caataaatat tgcggctcta
    14941 ttggaacaat aggcgtctat agttttgatc ctgtcaaaaa tataacaaca ggtgagggag
    15001 gaggaattac aaccagagat tcggaattaa tgactcgggc ccgacagttg cggtattgcg
    15061 gtattggaag cgcaggattt gaggctgcgg caatgaaaga gcgctggtgg gaatatgaca
    15121 tcgtagatgt ttttcctaaa cttcttccta atgacattgc ggcttcaatc ggactggcgc
    15181 agctacgcaa gatagataaa tttcaaacga tccgtaagaa aatttggaat atatatcaag
    15241 ctcaattagc tgatttcgac tggctgataa gacctcaaga ccctgcttca gatgaacagc
    15301 attcctattt cacttactcc attcgggttg ccaaaggaaa gcgcgatcaa ttggctaaat
    15361 acctctattc ccgcggtatc tatacaactg ttcgattcca tccattgcat ctgaatccta
    15421 tttataaatc atcttacagg cttcccattt gtgagcagct taacgatgaa atacttagtc
    15481 ttccacttca cccaaatctt tctgatagcg acattgatgc gattttatta agtttgcaaa
    15541 aatttaatga acagtacatg tgaatacagc gtccgatgtg atcactgtat ccacggaata
    15601 attgcgttct tcaaaggcac tggtaggtaa tcgtggttat tgctgaaggt cacatttgcg
    15661 ataaaagatg tgtcggcgca taagcagctg tgtgacccaa agaggccgtc agccgagagg
    15721 atcgtgcgcc gtattcgcag gccattcccg gacatgttga gggcagtgag cacaaggcag
    15781 ttattcttgt gacaaagcac tccaggggac aattgcaacg ttcgcgggga tcagagtagt
    15841 tcttccctga tctgcgcatc gtgcgttgca gagcattgtt cagggaacgc ccgcatgcca
    15901 cgtgtatccg tcatcatccc cgcctataat tctatgcagt gggtctctgc tgccatcgac
    15961 agcgtcctga accagggtta tcgggactac gaagtggttg tgattgacga tgggtcaacc
    16021 gatcagacgg cagacgttct tgagtcctac ggcagaaaaa tacgatgtat ttatcaagcc
    16081 aatgctggtg tatccaacgc tagaaaccgc ggcctgagcg tatcgaccgg cgaatttatc
    16141 gcgtatctgg acgccgatga tatgtggtat tcgcagaagc tcgaatgtca aatcgcgttc
    16201 ttggatgccc atccccaatg cggccttgtg cacagcgatg taaccgttat tgatgaacag
    16261 gagaaggtca tccgtgcgcg ctttaatgtg gaaaccgcta ggcccgttcc ctcaggctat
    16321 tgtctgcaag accttctgcg acgctgtcac atccaggttc catcggtcat tgaacgacgg
    16381 gagtgtatcg aagaggttgg tgggtttgat gagcaattta tggcaacaga agattatttg
    16441 cattggatta tggtgtcact gaagggatgg gaatttggat acatcaatga gccgttagcg
    16501 aagtatcgtt ggcgaggcgg aagcgtttcg tccaacaagc gactcttcct ggaggagtat
    16561 gggaagatgt ttggtgtact actgcccgct ttatacgaga atcgtcccca agcaccggat
    16621 ttggtcatca tacgcaagag atttctgcag gttgaacgcg aattggcata tatggattgc
    16681 atagaagggc atctgagtaa cgcgaggcag cgacttatga gactgataca gcgagcaccg
    16741 ttccaatata acctctattg ggacctggca aagacctact tgcgctagta cgaccgaaac
    16801 aaagctactt ctatttggat cacattcggg cacacctgaa gccgttggag gcatatcggg
    16861 gcagtccttg ctggtaagca ggcaagccat tccaggtgaa cctcgcaccc cttcctgctg
    16921 gttcaacatg attcctggct gatgtcagcg ctcaaagtcc ctttagactg acgtcgagtt
    16981 ccctgcgagt cgtcgtacgg cgtgagccgc tccactgcca cctgccgaaa ggtactgcaa
    17041 attcccccgc gcgtttcttc accatccgtc tggtgctaag tgcgccaatc cgttgcatct
    17101 cactctaggg gagcagtcca tggctgctcg tgcgagtgcc gatgtctgtg cgtattccgc
    17161 tgaatccggc caccgattcc gagcgaatcc ggccacccct cgg
//
LOCUS       DAORRQ010000074        16587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_184_length_16587_cov_22.716913, whole genome shotgun sequence.
ACCESSION   DAORRQ010000074 DAORRQ010000000
VERSION     DAORRQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16587
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184_length_16587_cov_22.716913"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..368)
                     /locus_tag="PK224_17445"
     CDS             complement(132..368)
                     /locus_tag="PK224_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRB17585.1"
                     /translation="MELHDRLEEVFRQVFDNESLTLTNEMTAPDIEGWDSVAHINLMF
                     GIEQAFGVRFKGNELAEMKNIDELKQFLIGKGAS"
     gene            complement(520..2091)
                     /locus_tag="PK224_17450"
     CDS             complement(520..2091)
                     /locus_tag="PK224_17450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB17586.1"
                     /translation="MAPAEAPSGAPPSTPHVTTVCFTTQDLAQFSAASGDRNPLHLSE
                     EYARATPYGEPVVFGMLGVLAAVGHLADRQDRLLQRLSVEFRNPLVVGMPYRLEALET
                     SVERACVKLYDATRLMLKATFTFVPGCEKSWDMHVPEAACFTEAEDRKKEDLPAGTRV
                     TGVYGPAISELVQVITRWRLSEKGATTRQLAAMLWASFVVGMQLPGKRAVFWRLELTL
                     YPEDGPQDTLLSYDVAVQDFDERFDLLHSAGTLSAAGTRCATADMWAFVRQDSPQPSL
                     RRVTDLIPRSDRLKGKVALVIGGSRGLGAAITQALASQGCTVFLNYHQCRTEAEKIRA
                     SLGDTSNLIELAQGDASEIEWCRTLRQRIVERYGGLDLLIGNASPPIRPLSFVPEKLA
                     QFQNFLTQSVSLASMPMSTFLNDLSERSGWNVLVSSAFVKDRPAEWPHYVTAKCAIEG
                     LVQWAAVHSPKVRTLIVRPPKLLTDQTNTTVGRQGAMEVERAAAAIVTRITCPDSSPT
                     VQILETFELNPEASQ"
     gene            complement(2078..4075)
                     /locus_tag="PK224_17455"
     CDS             complement(2078..4075)
                     /locus_tag="PK224_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HRB17587.1"
                     /translation="MNIARAQCAASSLTPETRGDAFRKKGQVEQAIEAYLEGVAAPPS
                     AALCLKLARCYEQMANYAEACRWALSIVDAGDDFTSWQAGWALFQRNAQKARRPAVRS
                     VKVALLGSYTTTQLCPMLCLAAGKLGIHIDLYESRYGQYQQDILDHKSGLYAFNPNIV
                     VLAVHEGDLHLPEYSQRPEEDVRKEVSRWTGLWQMVTEHSRARIVQHNFALPCEIPTG
                     HLTTRLSGSRYMMTQAVNTRLGEAAANAVSLVDCERLSALIGKQRWCDPRYWNLSKQA
                     VALEALPLLGRHTAAVIAADLGLSRKCLVLDLDNTLWGGVIAEDGLAGIKLGQGPDGE
                     AFVAFQEYLLKLKRKGVILTVCSKNNHADAIQPFEKHPEMRLKLQDIALFLANWEPKP
                     DNIRTIAKTLQIGLDALVFVDDNPVEREIVRKFLPEVDVIPLPEDPSYYLRTLSDYLL
                     LETSSLTAEDTERTDQYRARAQIMELEAAAGSIEDFYRSLRMQAIVVPFDASQLPRIA
                     QLIGKTNQFNLTTRRHGMAQLEAFVLDSSYVHLALRLRDRYTDHGLVSVMIARQQGDI
                     LDIDTWLMSCRVIGRTVETTMLEQLCRRAAQLGCTSLRGTYIPTQKNAMAADAYAKHG
                     FERVGNHEGPEVWTYDLAAKGLIANTFVKPVDSWELADGAC"
     gene            complement(4200..4820)
                     /locus_tag="PK224_17460"
     CDS             complement(4200..4820)
                     /locus_tag="PK224_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRB17588.1"
                     /translation="MSVRSILIHVTEVPMLGTLVRLYRGIQELRNQVIEDWTVNSRDW
                     TMPGFRAIAVHRLGTWLANRRSGVVKSGLLAVYRVLYRYVRNHYGIEIPLTVTIGRRL
                     WLVHQHGIVFHWKSEIGDDCLIRHGATIGAGYGGQKTLHKGPKLGQRVEVGPGAVIFA
                     GVKIGDDAVIGPNAVVMSDVPAGARVFAEAPRVIQLPTAHQAARKN"
     gene            5524..5850
                     /locus_tag="PK224_17465"
     CDS             5524..5850
                     /locus_tag="PK224_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRB17589.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFAVHGAVESARIITDKFTGQSR
                     GFGFVEMASSDEAQKAISALNGTDMGGRTLTVNEARPQEPRSGGGPGRSGGGGFNDRG
                     GKRDRW"
     gene            complement(5945..6649)
                     /locus_tag="PK224_17470"
     CDS             complement(5945..6649)
                     /locus_tag="PK224_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17590.1"
                     /translation="MRGWILAVLCGIPLWPGQSLPAEQEDPETSHTRQWDFDSIAPGT
                     LPSSFVIGTLFDGRPAGEWKILITDRAKSPSQVLAQLQPKGTDQAHKLLLMEGTDSGN
                     IDVEVSYLAVAGKADFGGGLVWHATDDRNYYLLRASSVEQKVRLYRVVKGVQQIVKQL
                     DRPLPANGWHKLRIVQRGCELKALYDDAVLFRVCDQTFSNGRIGLWTKADAVTYFDDL
                     VLRLLDDPLSNGRPPR"
     gene            complement(6842..7738)
                     /locus_tag="PK224_17475"
     CDS             complement(6842..7738)
                     /locus_tag="PK224_17475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17591.1"
                     /translation="MQDGVRGHSPARRVSPALILLVLGLTTFFPTYTSAAPLDKPVSK
                     PGMDDKPGLAEEQILSTTDKITQPVDTDAEAMRHNDLGVAFVFKGDLGQAIDEFKHAL
                     RLQPNYFAAHLNLANTLLDVGRNDAAMVEFKEALRLKPDDPKTHNDLGVALKEMGNLE
                     GAIAEFRTVLRHNPSDVNAHNNLGVTLKAMGDLDGAIAEYRTAASLQPNDVNAHFNLG
                     LGLMEKRQPEAAVSEFRTALHLRPNDAKIRLNLGNALAGMGQRMEAAQELRQYLRLEL
                     DTPANRRWLEQAEAKLRELEMP"
     gene            complement(7968..9488)
                     /locus_tag="PK224_17480"
     CDS             complement(7968..9488)
                     /locus_tag="PK224_17480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRB17592.1"
                     /translation="MKRVTSRRQLAISTVLVMLSLSASGCLQGSNYHRPPVETPADWT
                     RMASGPLAAGATDTMPEADWWQAFHNQELTQFVERALAQNHDVRRAVSRVLEGRASVT
                     TAGAGLYPQLNVQGSYSNIVVSKNTLAGLGLATGQQPGPQVFAKPGSSFDLWNGAADL
                     RWELDVWGRIRRGMEAASADAQAIEQDARAIALTLIGDVGQSYFRIRELDEQIEIAQR
                     ALTLRRDSLDIITKRASVGLASDLDVKRTEVLVAESAGQIPDLTRLRAVELHRLEVLT
                     GSAPGSVVLAPTSLRKVIVQPEIPVGLPSQLLERRPDILQAEASLVAANARIGQARAY
                     FFPTLSITGQGGLQSAEFANWFSGNSANFSIGPSVTLPIFLGGTNVARLEAAESRYQQ
                     MLEGYQQTILLAFREVADLLVSIHSRTEQLARQREQATAATAAVGLAEVRYRKGLVNY
                     LDVLDAQRTMLAAETQLAQTERARLTDMVGLYKALGGGWQTPGNNLSAIPAGSATH"
     gene            complement(9485..10477)
                     /locus_tag="PK224_17485"
     CDS             complement(9485..10477)
                     /locus_tag="PK224_17485"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17593.1"
                     /translation="MTILNRFSVWLAVAGAMLAAWVVLTAGKNQPMPTPIVEPPRSPY
                     ETTVAATGIIEAANENVRIGPPMAGLVTKVFVAVGDRVREGDPLLQLDDRDLRAQLVA
                     RQAAIPPAEAQVEEQKYRIGDLDTQLKRLKSVRDSRAVSEDDVKRTWYASEMAKRALS
                     RYEATLKQVIAQRNETQMLLDRLTVRAPRAATILQVNVRAGEFALTTGATEPLMLLGD
                     MQQLQVRAEVDEVNAPLVAPNRPGLAYLKGNTAQAIPLTFVRIEPYIVPKKSLTGDNS
                     ERVDTRVLQIIYRFERPAFPIYAGQQVDVFIERAPDGPPAPAPGSARSAEEPSK"
     gene            complement(10474..11232)
                     /locus_tag="PK224_17490"
     CDS             complement(10474..11232)
                     /locus_tag="PK224_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB17594.1"
                     /translation="MDGSSQSQGRTTEGNGAEQTIAVQVRGLVKSFGSGETTVTVLKG
                     IDLDVYFGELLLLVGESGGGKTTLLSAIAGILDIDAGELDVLGASLTNMSPGTRTRFR
                     GQTMGFIYQQFNLLPALTAAENVAIPLLIQGTRKKEAISRGRLMLERVGLGDRTEFLP
                     RNLSGGQQQRVAIARALVNEPQLLVCDEPTAALDGPNGQKIMELIRDVGRAPDRCVIV
                     VTHDSRIFQFGDRMAELTDGRIVGIHPIQREATA"
     gene            complement(11238..12368)
                     /locus_tag="PK224_17495"
     CDS             complement(11238..12368)
                     /locus_tag="PK224_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB17595.1"
                     /translation="MNYVALRMLFGDRAKYLMLLCGLTFAVMLIVQQGSIFWGLMIWS
                     QSSISNLNVPIWVTDPGIAQVDEVKPIADTTVDRVRSIPGVEWAVPLYKGLLRARLAN
                     GEYHQITLTGLDAATLIGRPAEVLEGRFEDLRQPDAVALDQWAVERMGGPTVITVGTV
                     FELNDKLARVVAIAKTQKTFTNIPVVYTTYERALRYVPRERRTLSYVLAKAKDSAPVD
                     EVIQRIRDHTGLGAFTADAFGWKTIGWVLKNTGIGINFGTTILLGFVVGMAIAGQTFY
                     LFTVENLRQFGALKAMGASTATLARMILLQAFTVGITGYGVGIGLATVFGLLTAQEGQ
                     LPFLETWPLLLLVFVALLMICTLSALISIIKLARLEPAIVFR"
     gene            complement(12365..12781)
                     /locus_tag="PK224_17500"
     CDS             complement(12365..12781)
                     /locus_tag="PK224_17500"
                     /inference="COORDINATES: protein motif:HMM:NF039959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6022 family protein"
                     /protein_id="HRB17596.1"
                     /translation="MKNVGSRLTPDTVSHEIEAYVAARQAELLPSGEASKDGRRFSWC
                     GEILDFLTEGLAERLAAKGITFTDAFPGPLRLMDEDQQPNGQHVRRFWTVALHHGCPI
                     ARICTYFFHRHDQVSLPQLPRVVAYAPTGPAQEEHE"
     gene            complement(12958..15000)
                     /locus_tag="PK224_17505"
     CDS             complement(12958..15000)
                     /locus_tag="PK224_17505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB17597.1"
                     /translation="MGTSTDRRDRFRILNALLQAKTLRQFDERLQQELAPLLGCEIIG
                     LYLYSESTAAFSPVSESLRTPASPAYPIDQLPAAGTIKEAAVLAGQAIFTHDLSASPW
                     AEAQAIDPTTCQATSVLVSPIRIPAESRPCATAKTLAVMVAVSPGRPGLFSEEDRVFL
                     DMLGLHIAPVLTAVLAAEERDTLVAINSQVVLGTMTLDNLLSSIQPILREVIPHDTTG
                     LVRFIGAPHGPWFEILSCEGVAVDQEALKQFPFERMAPAEILATGKPLLLTGYNQERF
                     AEHTYFESLGVFSAMLCPLLVRGAPYGFLAIGSRRRNAFSERDLALAEQIGFHLSHAI
                     ANLSAYDQIRQLKDQLEQENVYLREEMGASLDLTSLIGNSAALQKSLKAIEQVAPTDS
                     TVLITGETGTGKELVAQAIHRLSPRQHKALITVNCAALPPTLIESELFGHERGAFTNA
                     TSRKLGRFELAHGGTIFLDEVGELPIDLQMKLLRVLEAQEFNRVGGGHTIRVDVRVLA
                     ATNVDLDQAIKRGSFRADLFYRLNVFPLRLPTLRERREDIPLLARHFVKKYSQRHRKP
                     VTRINSAALNALSGYDWPGNVRELEHVIERAVIVSQGSAITVDELDGLGRGEAPSPEP
                     RTLAEAERAHIVETLSRTNWILAGKQGAAEKLGLKRSTLQHRMKKLDISRPPRQAS"
     gene            complement(15069..>16587)
                     /locus_tag="PK224_17510"
     CDS             complement(15069..>16587)
                     /locus_tag="PK224_17510"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HRB17598.1"
                     /translation="LLNQGLDLSLRTGSHVPKMALSATEIGSIVQELAPALADGWIQK
                     ISQPLPDGLLLEIRVPGHTRRLLCSLRDGTARIHLVRESLPNPPTPPSFCQLLRARIQ
                     GARIDGITHIPGDRIVRLDLTSRDGPVTLIAELFGRNADLLFLDGATRVLATLRHNRE
                     RVGQVYQAPASVPLRSSPSDTATPAPEPQPPTEADPFPLSSRLEILYREREAELSHLT
                     QVRQRESALRKALKKLLRRMEGLRRDLEQAGRYEPYARYGELLKANLGLLKKGMTAVS
                     VVDYYDERLPELSIPLDPTKGPQANMDAYFAKYRKFVSAQREIAPRVAAIDTDVQQLQ
                     AELATIKNGTWQAPSSERRATAGAMPRPGRKNAADEKRRGPFRRFISSDGHPIFVGRN
                     ARENDELTFGLAKSEDLWLHARGTPGSHVVVRLEKGTDPPPETIRDAATLALLYSDLK
                     KSGKGDVMYTRRKWVKKAKGQAPGAVTVTQEKTIYITLDKTRLAALKARNTEGGP"
BASE COUNT         3150 a         4737 c         5134 g         3566 t
ORIGIN      
        1 agcatgcgtc gtccccaggc gatagcgctt ggagagaccg gtaacgctga cagcggtgtt
       61 gctcatctca atcagatcgg cgaaggttcc tgttgcttcg ttcgttgatc gacccgtggc
      121 tgcggtatgg gttagctggc tcccttgccg atcaggaatt gcttgagttc gtcgatattc
      181 ttcatttcgg ccaactcatt gcccttgaac cgaactccga acgcctgttc gatgccgaac
      241 atcagattga tatgggccac ggagtcccag ccttcgatat cgggtgccgt catctcattg
      301 gtcagcgtga ggctctcatt gtcgaacact tggcgaaaca cttcttcaag tcgatcatgc
      361 agctccattc attcccccaa ctctcagtgg tgtcgtcaca tctccatcat cgtgtcgtgg
      421 ccggaaagcc atcttcccgt agcccctgct tctgtaaagt cgcttcaaac agccgaggca
      481 tgaggtcaat ctgcttcgtc gtgcaatggt cccggaggtt cattgcgaag cctcaggatt
      541 cagctcaaac gtctcgagta tctgtacggt gggtgatgag tcggggcaag tgatccgtgt
      601 gacgatcgca gcggcggcgc gttcgacctc cattgctccc tgccgtccca ccgtggtgtt
      661 ggtttgatcc gtcaacaact tcggtggtct aacaataagc gtacggacct tcggagagtg
      721 aactgcggcc cactgcacca ggccctcgat cgcacatttc gcagtgacat agtggggcca
      781 ttccgcagga cgatctttca caaacgccga agagacaagc acgttccagc cggagcgttc
      841 ggacaagtcg ttgagaaacg tggacatggg catactggcc agcgagacac tctgcgtgag
      901 gaagttctga aactgcgcga gcttctcagg cacgaacgac agcgggcgaa tgggcggcga
      961 tgcattgcct atcagcagat caagtccgcc gtatcgctcg acaattctct gccgtaacgt
     1021 tcggcaccat tcgatctccg acgcatcccc ctgagccaac tcaatgaggt tggaggtgtc
     1081 tccaaggctc gctcgaatct tttccgcctc cgtcctgcac tgatggtagt tcagaaacac
     1141 cgtgcagccc tgcgaagcca gcgcctgagt gattgccgca ccgagtccac ggcttccacc
     1201 gatgactaac gcgaccttcc ccttcagccg gtctgatctc ggtatcagat cggtaacccg
     1261 acgaagggag ggctgcggag aatcctgtcg tacaaaagcc cacatgtcgg ccgtcgcaca
     1321 acgggtgccg gctgccgaga gagtgccagc actgtgcaag agatcaaatc gttcgtcgaa
     1381 gtcttgaacc gccacatcat aggaaagcag cgtgtcctgt ggtccgtctt ctggatacaa
     1441 ggtgagttcc aatcgccaga agacggcgcg cttgccggga agctgcattc cgacgacaaa
     1501 gctggcccac agcatcgccg cgagctgtct cgttgtggcg cctttttcgg agagtctcca
     1561 tcgagtgatc acctgtacca gttcgctgat tgccggcccg tagacgccgg tcacacgggt
     1621 acctgccgga aggtcctcct tcttccgatc ctctgcttcc gtaaaacagg ccgcttcagg
     1681 aacgtgcatg tcccatgact tctcacaccc aggaacaaat gtgaacgtcg ccttcagcat
     1741 caggcgcgtg gcgtcataga gtttgacgca cgcccgttct accgaggttt cgagcgcctc
     1801 cagacgataa ggcatgccga cgacgagcgg atttcgaaac tcgaccgaga ggcgttgcag
     1861 gagccgatcc tgacggtcgg cgagatggcc gactgccgcc agcacgccaa gcatgccgaa
     1921 taccacgggc tcgccgtagg gtgtggctcg cgcatattct tcggagagat gtagggggtt
     1981 tctatccccg ctggctgcac tgaactgggc gagatcctgc gtcgtgaaac acactgtggt
     2041 cacatgtggc gtggaggggg gagccccgga cggcgcttca gcaggcgcca tccgcgagct
     2101 cccaggagtc gacaggtttc acgaacgtat tggcaatcag gcctttggcc gccaggtcat
     2161 aggtccagac ttcgggtcct tcatggttcc cgactcgctc gaagccatgc ttggcataag
     2221 cgtccgccgc catcgcattc ttttgcgttg gaatgtaggt gcctcgaagc gaggtgcatc
     2281 caagctgcgc ggcgcgccga cagagctgtt ccagcatcgt cgtctccacg gtccggccga
     2341 tgacccggca gctcatgagc cacgtgtcga tatcgaggat atctccctgt tgtcgcgcga
     2401 tcatcacgct gaccagtcca tggtcggtgt agcgatcacg caaccgcagc gccaaatgga
     2461 cataactcga gtccaggacg aaggcttcca gttgcgccat gccgtgacgt cgagtggtga
     2521 gattgaattg gttggttttg ccgatgagtt gggcaatgcg cggcaactgt gatgcatcga
     2581 acggcaccac gatggcctgc atccgaaggc tgcggtaaaa atcttcaatg gaaccggcag
     2641 ccgcttccaa ctccatgatc tgcgctctgg cccgatattg gtcggtccgc tccgtgtctt
     2701 cggcggtcaa tgagctcgtc tcgagcagga gatagtccga cagagttctg aggtaatagg
     2761 aggggtcttc cggcagcggg atgacatcga cttcgggtaa gaacttgcgc acaatctcgc
     2821 gctcgacagg gttatcatcg acgaagacca acgcatccaa accgatttgc agcgtcttgg
     2881 cgatggtgcg gatgttgtcg ggcttgggct cccaattggc aaggaagagg gcgatgtcct
     2941 gcagcttcag tcgcatctcc ggatgttttt cgaagggttg tatggcatcg gcgtggttat
     3001 tcttcgagca gaccgtcagg atcacgcctt tgcgtttgag cttcagcaga tactcctgaa
     3061 acgccacaaa cgcctcgccg tccggccctt gccctaactt gatgcccgcc aatccgtctt
     3121 ccgcaatgac accgccccat aacgtattgt ccaaatccag caccagacat ttacggctga
     3181 gcccgagatc cgcagcaatc acagccgccg tatgtcttcc gagcagaggc agggcctcca
     3241 gtgcgactgc ttgtttcgac agattccaat agcgcggatc gcaccatcgc tgctttccaa
     3301 tgagcgccga aagacgctcg caatctacca gggagacggc gttcgcagcc gcttcaccca
     3361 gcctcgtatt gacggcctgc gtcatcatgt atcgagagcc ggacagcctg gtggtcagat
     3421 ggcctgtggg gatctcgcag ggcaatgcaa agttatgttg cacgattcgg gcgcgggagt
     3481 gttcagtcac catctgccag aggccggtcc agcgactcac ttctttgcga acgtcttctt
     3541 cagggcgttg gctgtattca ggaagatgga ggtcaccctc gtgaaccgca aggacgacga
     3601 tattcgggtt aaacgcatag agcccgctct tgtggtcgag aatgtcctgc tggtattgcc
     3661 cgtatcggct ttcatacagg tcgatgtgga tgccgagttt ccccgccgca aggcacaaca
     3721 tcgggcacag ttgggttgtg gtatagctcc cgaggagcgc gactttgacc gacctgaccg
     3781 ccggtcgccg ggctttctgt gcattgcgtt gaaacaatgc ccagcctgct tgccaggacg
     3841 taaagtcatc gcctgcatcg acgatggaaa gagcccatcg acaggcctcc gcatagttcg
     3901 ccatctgctc gtaacatcgc gcaagtttga ggcacagcgc agccgatggg ggagccgcca
     3961 cgccctccag ataggcctcg atcgcctgtt cgacctgacc cttcttgcgg aaggcgtcgc
     4021 cacgtgtctc aggcgtgaga gatgaagcgg cgcattgtgc ccgggcaata ttcatgaggg
     4081 acggcctgtc atttctataa gcagtacact caccggataa gaggcgtctg tggttgagtc
     4141 gatccctgga cggtgaatag cgcgaggttc cttcggagtc agtctccggc cgtaggtcgt
     4201 tagttcttgc gagctgcctg atgagcggtc ggcaactgta tgacccgagg cgcctcggca
     4261 aacactcttg cgccggccgg aacatccgac atcaccaccg cattcgggcc gatgaccgca
     4321 tcgtcgccga ttttgactcc ggcaaagatc accgcgccgg ggcccacttc cactcgttgt
     4381 ccaagtttcg gacccttgtg caacgtcttt tgcccgccat atcccgcccc gattgtggcg
     4441 ccgtggcgaa tcaggcagtc atctccaatc tctgatttcc aatgaaagac gatcccatgt
     4501 tggtggacaa gccagagtct ccggccgatt gtgaccgtca atggaatttc aatcccgtaa
     4561 tgattgcgca catagcgata cagcacacgg tacacggcga gcaggccgga cttgacaacg
     4621 ccgctgcgcc ggttcgcgag ccaggtgccg aggcgatgca cggcgatcgc tcgaaagcca
     4681 ggcatcgtcc agtcgcggct gttcacggtc cagtcttcaa tgacctggtt tcgtagttcc
     4741 tgtatgcctc gatagagcct gacgagtgtc cccagcatcg gcacctcggt cacgtgtatg
     4801 aggatggatc gaacgctcat gtggtgctgt cttgtcgctg agtcttcgca ggtgaggagt
     4861 ccaacgagat ctgagcaacc ggcgaagtgg ctggggctcc aaccgcatga gccatgatcg
     4921 gtggggcttc gcgagttgtg gagaatttcc tgagcagggc gctaggcgtc atgatccggt
     4981 agctgtgcta gcgagtgggc gcgggcggag tcgctcggca tagttccatt cggcaagagc
     5041 ctggcttcag gcctaatact ccgtgttgaa gacatggacg tatcttagca tatgtccagt
     5101 attactggac aagataaaat ccgcggtgcg ggcaaggaat gtgatgtttt ttggtgtgcg
     5161 attgttttgc ggtcggtttc tgtgcccgct gtgagctttg tgtcagattc agtcgtcgtt
     5221 cttttatgcc tggttgtcga ttgtgatgtg acagtcaatc ctggtcacaa cggcagaccc
     5281 gcaccttagt aggcctattt catggcttgt atggtggggg gacccatggt actatcccgc
     5341 gtctcccgct ggggcctggt gagagatgcg ttgaaatagt tcaggcctga ctcgatgttg
     5401 ccttgcccgg gaatctaatc gaaagccaga cccaagtcgc cccgttgtcc agcgtcttcg
     5461 gcccgtcctt tccctttctt tccttcgttc aagacaatgt cactacccaa gaggaggacc
     5521 cacatgggtt ctaaaattta tgttggcggg ttgccctatt cggcaaccga acagcagtta
     5581 agcgatttgt tcgcggtgca cggggcggtt gaatcggccc gtatcatcac ggacaagttt
     5641 accggccaat cgagaggttt cggattcgtc gaaatggcct cttcagacga ggcacagaag
     5701 gcgatttcgg ctctcaatgg gacggacatg ggtggccgga cattgacggt caatgaagcg
     5761 cgtcctcagg agccccgatc tggcggtggc cctgggcgga gcggcggcgg gggattcaac
     5821 gatcgcgggg gcaagcgcga tcgctggtaa cgcctaattt cgtatcagct tgtggggcag
     5881 tcatctttgt ggtggctgcc ccttttttgt gcctgccggt agccggtgaa ggtgcggtgg
     5941 ctgctcagcg cgggggacga ccgttcgaga gggggtcatc gaggagccga aggaccaggt
     6001 cgtcgaaata ggtcacagca tcggctttcg tccagagccc aatccgtccg ttggaaaacg
     6061 tctggtcgca cacgcgaaag agcacggcat cgtcatagag ggctttgagc tcgcagccac
     6121 gttgtacgat gcgcaatttg tgccacccgt ttgcggggag cggacggtcg agctgcttga
     6181 cgatttgctg gacccccttc acgacgcgat agagcctgac cttctgttca accgagctgg
     6241 ccctcaggag gtagtaattg cggtcatccg tggcatgcca tacaagtccg ccgccgaaat
     6301 cggcttttcc tgccacggcc aaataggaga cctcgacatc aatgttgccg ctgtcggtcc
     6361 cctccatgag cagcagcttg tgcgcctgat ctgttccttt cggttgcagt tgagcgagta
     6421 cttgggaggg gctcttcgcg cggtcggtga tgagaatttt ccactcgccg gccgggcggc
     6481 cgtcgaagag cgtgccgatc acgaacgaac tggggagcgt tcccggagcg atggagtcaa
     6541 aatcccattg cctggtgtgg gatgtctccg gatcttcctg ctctgccgga agggattggc
     6601 cgggccacag aggtatcccg cacaggacgg ccaagatcca gcctctcacg tgcgtgaatc
     6661 tgccgatggt gggcccagtg aaatggttgt gcatcaaagt ttcccgtcag gacggaagcg
     6721 ttgaggactg tacaccaaat gaggtgggat cggcgagagg agagtgagac cattcagtcg
     6781 gatcggcgat gactcccgga ttcggattcc ggcctcgctg gaccggccag cgaggcgccg
     6841 gttacggcat ctccagttca cggagtttgg cttcggcctg ttccagccac cgacgattgg
     6901 cgggcgtatc aagttcaagg cgaaggtact gtctcaattc ctgagccgct tccatccgct
     6961 gtcccatgcc tgccagggca ttgccgaggt tgagacggat cttcgcatcg ttgggccgca
     7021 ggtgcagcgc ggtgcggaac tcgctgacgg ccgcttccgg ctggcgtttt tccatcagtc
     7081 ccagccctaa attaaagtgc gcgttcacgt cgttcggttg aaggctcgca gcggtgcgat
     7141 actcggcgat ggctccatcg agatcaccca tggctttgag cgtcacgccg agattgttgt
     7201 gcgcgttcac atcgctgggg ttgtggcgca agaccgttcg aaactccgca atggcgcctt
     7261 ctaagtttcc catttccttg agcgcgactc ccagatcatt gtgggttttg gggtcgtcgg
     7321 gtttgaggcg cagggcttct ttgaattcaa ccatcgctgc gtcgttccgc ccgacatcga
     7381 gcagggtatt agccaggtta aggtgggcgg cgaagtagtt gggctggagt cggagcgcat
     7441 gtttgaattc atcgatggct tgccccaggt cgcccttgaa gacaaaggcc acgccgagat
     7501 cgttgtggcg catcgcttcc gcgtctgtgt ctaccggttg cgtgatttta tcggtagtcg
     7561 aaagaatctg ttcttctgcc agccccggct tgtcgtccat cccgggtttg gagaccggct
     7621 tgtcgagggg cgcagccgac gtatacgtcg ggaagaacgt cgtgagcccg agtaccaaca
     7681 gtatgagggc aggagaaacc cggcgtgccg gggaatggcc gcggacaccg tcttgcatga
     7741 aaggctcctt ccgagaagtc gtacgaagaa tctgacgaga gcgcgcgacc ggtgcctggt
     7801 ccgcaactct attgaaggat tcggattgca ttccgtcaaa tagccctatc cgcgcgaaaa
     7861 attcaagttg aaagtccctg ctgtggtgac gatgcgagtg tgaggcagcc ggcaatgcga
     7921 ttgctgactg cgccggagga aggactaaca ctggccgtgt ggattgctta atgcgtggcg
     7981 gagccggccg ggatcgcaga gagattgttg ccgggcgtct gccatccgcc gccgagggct
     8041 ttgtagaggc cgaccatgtc ggtgaggcgg gcgcgctcgg tctgcgcaag ctgtgtttct
     8101 gcggccagca tcgttcgttg ggcatcgagg acgtcaaggt agttcaccaa ccccttgcga
     8161 tagcgtacct ccgccagccc tacggccgct gtagccgccg tcgcctgttc acgttggcgc
     8221 gcgagttgtt ctgtgcgtga gtgtatcgac accaggaggt ccgctacttc gcggaaggcg
     8281 agcaggatcg tctgttggta accttccaac atttgttgat atcgagattc ggccgcctcc
     8341 agtctcgcga cgttggtccc gcccaggaaa atggggagtg tgaccgatgg accgatactg
     8401 aaattcgcgc tgtttcccga gaaccagttt gcgaactcgg cgctttgtag gccgccctgc
     8461 cccgtgatgg acaatgtggg gaagaaatag gcccgcgcct gaccgatccg ggcgttggcg
     8521 gccacgagcg acgcctccgc ctgaaggatg tcgggtcgcc gctccaacaa ttgcgacggg
     8581 aggccgacgg gaatttccgg ctgtacgatg accttccgca aggatgtcgg cgccaagact
     8641 acgctgcccg gcgccgatcc cgtcaacact tccaaccggt gtaattcgac ggcgcgcaga
     8701 cgagtcagat ccgggatctg ccccgcgctt tccgcgacga gaacttccgt tcgttttacg
     8761 tccaggtcgg aggccaggcc cactgatgcc cgcttggtaa tgatgtcgag cgaatcccgt
     8821 ctgagggtca gggcgcgttg ggcgatttca atttgctcat ccagttcacg gattcgaaag
     8881 tatgactgtc ccacatcccc aatcagggtc agcgcaatcg cgcgggcgtc ctgctcgatc
     8941 gcctgcgcgt ctgccgaggc ggcttccatc ccgcgacgga tgcgacccca gacatccagt
     9001 tcccatcgga gatccgccgc cccgttccag agatcgaaac tgcttccggg cttggcaaag
     9061 acctgcggcc ccggttgttg gccggtggct aagcccaatc ctgccagggt gttttttgac
     9121 accacgatat tgctgtagct gccctgcaca ttcagttgtg gatagaggcc ggcgccggcg
     9181 gtggtaacag aagcccgtcc ctccagcacg cgtgacacgg cgcggcggac gtcgtggttt
     9241 tgtgcgagcg cgcgttccac gaactgggtc aactcctggt tgtggaaggc ctgccaccag
     9301 tccgcttccg gcatcgtatc ggtcgctccg gcagccaatg ggccggaagc catgcgtgtc
     9361 cagtcggccg gtgtttcgac cggtggacga tggtagttcg agccctgtag gcacccgctg
     9421 gcggacaggg acaacataac gaggacggtg gaaatggcga gctgtcggcg ggacgtgact
     9481 cgtttcattt gctcggttcc tcagcggaac gtgcgctgcc cggtgctggt gccggtggtc
     9541 cgtccggtgc gcgttcgatg aacacatcga cctgctgtcc ggcatagatc ggaaacgccg
     9601 gccgttcgaa gcggtagatg atctggagga cccgcgtatc gacccgttcg ctgttgtctc
     9661 cggtcaggga ttttttgggg acaatataag gctcgatgcg cacgaatgtc agcggaatgg
     9721 cctgggcagt attgcccttc aggtaggcga gcccgggtcg gttgggagcc accaatggag
     9781 cattgacctc atcgacctct gcgcggactt gcagttgctg catatcgccc aggagcatga
     9841 gcggctcggt cgcaccggtg gtcagggcga attcaccggc ccggacattg acctgcaaaa
     9901 tcgtcgcggc ccgcggcgca cggactgtca ggcggtccag gagcatctgt gtttcgttgc
     9961 gctgggcgat gacttgtttg agggtggcct cgtagcggct cagcgcgcgt tttgccattt
    10021 ccgaggcgta ccaggtacgc ttcacgtcgt cttcgctgac cgcccggctg tcccgaaccg
    10081 atttcagtcg cttgagctgc gtgtcgagat cgccgatgcg atatttctgc tcttcgactt
    10141 gggcctccgc cggtggaatc gcggcctggc gagccaccag ttgcgcgcga aggtctcgat
    10201 cgtccaattg aagcaaggga tcgccctcac gaacccggtc accgaccgcc acgaacactt
    10261 tcgtcaccag tcccgccatc ggcggcccga tacggacgtt ttcgttggcc gcttcgataa
    10321 tgccggtcgc ggcgacggtc gtttcatagg gagaccgggg tggctctact atcggcgtcg
    10381 gcataggctg gttcttgccg gcggtcagga cgacccaggc agccagcatc gcgcctgcga
    10441 cagcgagcca gacactaaag cgattgagaa tggtcatgcg gttgcctctc tctgaatcgg
    10501 gtgaatgccg acaatgcggc cgtcggtcaa ttccgccatg cgatcaccga actgaaaaat
    10561 ccggctgtca tgggtgacga cgatgacgca gcgatcgggg gcacgcccca catcccgaat
    10621 caattccatg atcttctgcc cattgggccc gtccagcgcg gcggtgggtt cgtcgcacac
    10681 cagcagttgc ggctcattga ccaacgcccg ggcaatggcg acgcgctgct gctggccgcc
    10741 ggagaggttg cgcggcagaa actcggtccg gtctccgagc ccgacacgtt caagcatcag
    10801 ccgccctcgt gagatcgcct ccttcttccg agtgccttgg atcaagagcg gaatggcgac
    10861 attctccgcc gccgtcaagg cgggcaggag attgaattgt tggtagatga accccatggt
    10921 ttgcccacga aaacgggtgc gcgtgcctgg cgacatgttg gtcagtgagg ctcccaacac
    10981 atcgagctca ccggcatcga tatccaggat tccggcgatg gccgacagca gcgtggtctt
    11041 tcctcccccc gattctccga cgaggagcag cagctcgccg aaatacacgt cgaggtcgat
    11101 gcccttgagc accgtgacgg tggtctcgcc ggacccgaac gacttgacga gtcctcggac
    11161 ctgcacggcg atggtttgct cggctccgtt gccttccgtt gtgcggccct gtgactgtga
    11221 cgaaccatcc atggcggtca tcgaaacaca attgccggtt caagccgggc cagtttgatg
    11281 atgctgatca gcgccgacaa tgtgcagatc atgagcaggg caacaaagac caacaggagc
    11341 agcggccagg tttccaggaa cgggagctga ccctcttgtg ccgtcagtag accgaagacg
    11401 gtggcaagtc cgatgccgac cccataccca gtgatcccca cggtaaacgc ttgcaggaga
    11461 atcatgcggg ccagagtcgc ggtggaagcg cccatggctt tgagcgcgcc gaactgccgc
    11521 aggttttcga cggtaaagag gtaaaacgtt tgccctgcga tggccatccc gaccacgaag
    11581 cccaacagaa tcgtggtgcc gaaattgatt ccgatgccgg tgttcttcag gacccagccg
    11641 atcgttttcc atccgaatgc atcggcggtg aaggcgccca gtcccgtgtg gtcgcgaata
    11701 cgttggatca cctcgtccac cggcgcgctg tctttggcct tcgcgaggac gtaggagagg
    11761 gtgcgccgtt ctcgcgggac atatcgcaag gcccgctcgt aggtggtgta gacgacggga
    11821 atgttggtga aggtcttttg cgtcttggca atggcgacga cgcgggccag cttgtcgttg
    11881 agttcgaaga ccgtgccgac cgtgatgact gttggcccac ccatgcgttc gacggcccat
    11941 tgatcgaggg ccacggcgtc aggttgccgg aggtcttcga atcgtccctc cagcacttcc
    12001 gctggacggc cgatcaaggt ggcagcgtcc agtccggtca aggtgatttg gtgatactcg
    12061 ccgttcgcaa gcctggcccg taagagcccc ttatacaagg gaacggccca ttccacaccg
    12121 gggatgctac ggacgcgatc gaccgtggtg tcggcaatcg gtttgacttc atcgacctgc
    12181 gcaatgccgg gatccgtgac ccagatcggc acgttgagat tactgatact cgattgggac
    12241 cagatcatga gcccccaaaa aattgagcct tgctgaacga tcaacatgac cgcaaaggtg
    12301 aggccgcaca gcagcatgag gtatttcgct cgatcgccga agagcattct cagggcgacg
    12361 taattcattc gtgctcttcc tgagccgggc cggtcggggc ataggccacc acgcgcggca
    12421 attgcggaag gctcacttga tcatgtcgat gaaaaaaata ggtgcagatc ctggcgatgg
    12481 gacagccatg gtgcagggcg acggtccaga aacgccgcac atgttggccg ttcggttgtt
    12541 ggtcttcgtc catgagccgt aaggggcctg gaaaggcatc ggtgaaggta atgcctttgg
    12601 cggcgagtcg ctccgcaagt ccttcggtca gaaagtccag aatttccccg caccaggaga
    12661 accgccgccc gtctttgctt gcctcgcctg acgggagcaa ttcggcttgt cgcgcggcga
    12721 cataggcctc aatctcatgg ctgacggtgt ctggtgtcag tctggaaccg acgttcttca
    12781 tgtaggaata ccagagcagg ggtgatgcca gcgcggaatg cggagtcact tttgcgagca
    12841 agttactaaa agataaggag aatagagagt aatcgagaaa cgtgaccgga gtagatgatt
    12901 agggcgaagt gccgagagtt gggcaaggct tagaggtgca tgcccacata cgggcactca
    12961 cgaggcctgt cgaggcgggc ggctgatgtc cagcttcttc atgcggtgtt ggagcgtgga
    13021 ccgtttcagt cccaatttct ccgccgctcc ttgcttaccc gccaggatcc aattggttcg
    13081 agagagtgtc tcaacgatgt gcgcacgttc agcttctgcg agcgtccgcg gctcgggaga
    13141 gggtgcttct ccgcggccca atccatccag ttcatcgacc gtgatcgccg agccttggct
    13201 cacgatgacg gcacgctcga tcacatgttc caattcccgg acattcccgg gccagtcata
    13261 gccggacagg gcgttcaggg ccgcactgtt gattcgcgtg accggctttc tgtggcgctg
    13321 gctgtatttc ttcacaaagt ggcgggccag tagcgggatg tcctcgcggc gttcgcgcaa
    13381 ggtcggaagg cgcagcggga agacgttgag gcgatagaag agatcggcgc gaaatgagcc
    13441 tcgtttgatg gcctgatcca gatcgacgtt ggtggcggca agcacgcgta catcgactcg
    13501 aatggtgtgg cctccaccaa cccggttgaa ttcctgggcc tccagcaccc gcaggagttt
    13561 catttgtagg tcgatcggaa gttctcccac ttcgtcgagg aaaatcgtgc ccccgtgcgc
    13621 caattcaaag cgccccaatt tgcgggatgt tgcgttggtg aaggcgcccc gctcgtgacc
    13681 aaagagttcg gattcgatga gcgtgggcgg gagcgcggcg cagtttacgg taatcaacgc
    13741 cttgtgttgg cgcggcgaga gtcgatgaat cgcttgagcc accagttcct tgcctgtccc
    13801 ggtttccccg gtgatgagga cggtggaatc ggtcggcgcg acttgctcaa tggccttcag
    13861 cgatttctgc agcgcggcac tgttgccgat caggcttgtc aggtcgagag aggcacccat
    13921 ctcctcacgc agatagacat tttcctgttc cagctgatcc ttgagctgac ggatctggtc
    13981 gtaggccgag agattggcga tggcgtgcga caagtgaaag ccgatctgtt cggccagcgc
    14041 caggtcgcgt tcggagaagg cgttccgccg tctgcttccg atcgccagaa agccgtacgg
    14101 cgctccccgg acgagcagcg ggcagagcat ggcagagaac acgccgagtg actcgaaata
    14161 cgtatgttcc gcaaatcgtt cctggttgta tccggtgagc agcaggggct ttccggtggc
    14221 caggatttcg gccggcgcca tccgctcgaa gggaaattgt ttcagcgcct cctgatcgac
    14281 ggcgacgcct tcacaggaca ggatctcgaa ccaggggccg tggggggcgc cgatgaacct
    14341 cacgaggccg gtcgtgtcgt gagggatgac ctcacggaga atgggctgta tcgagctcag
    14401 gagattatcc agggtcatgg tgcccagtac gacctggctg ttgatggcga ccagggtgtc
    14461 gcgttcttcg gccgccagga ccgcagtcag gaccggtgcg atatgaaggc cgagcatgtc
    14521 gagaaacacc cggtcctctt cagaaaagag accgggtcgt ccaggcgaga cggcgaccat
    14581 cacggccaag gtcttggcgg ttgcgcaggg ccgggattca gccggtatcc tgatcggtga
    14641 caccaggacc gaggtggctt ggcaggttgt ggggtcgatg gcctgcgcct cggcccaggg
    14701 cgaggcactc aagtcgtgtg tgaagatggc ctgcccggca agcacggctg cttctttgat
    14761 ggtaccggcg gcgggcaact gatcgatcgg gtaggcgggc gacgccggtg tgcggaggct
    14821 ttccgagacg ggcgagaagg ccgccgtgct ttcactatac aggtagaggc cgatgatctc
    14881 gcagcccagg aggggcgcga gttcctgctg gagacgttca tcgaactgcc gaagcgtttt
    14941 tgcttggagc agcgcattca gaatacgaaa tcgatctcgt ctgtctgtgc tggtgcccat
    15001 atgtgggcac tctaccattt ctcgaacatt gagggaaagg cggcgcggag caggttgaag
    15061 tgtttcgtct agggccctcc ttcggtgttt ctcgctttga gcgccgcgag acgcgttttg
    15121 tcgagggtga tgtagatggt tttctcttgg gtgacggtca ccgcgcccgg tgcctggccc
    15181 ttggctttct tgacccattt gcgtcgagtg tacatgacat cacccttccc gcttttcttt
    15241 agatcgctgt acagcagggc gagcgtggct gcgtcccgta tcgtctcagg cggcggatca
    15301 gtacccttct ccaggcgcac aacgacatgc gagcccggcg tgccgcgcgc atgtagccag
    15361 aggtcctcgc tcttggcgag gccgaacgtg agttcgtcat tttcgcgggc gttgcgcccg
    15421 acaaagatcg gatggccgtc cgatgagatg aagcgtcgaa acggtccccg gcgtttctcg
    15481 tcggcagcgt ttttccgacc ggggcgtggc atcgcaccag ctgtcgctcg tcgctcacta
    15541 ctcggtgcct gccaggttcc atttttgatg gtcgctaatt cggcctggag ttgctggacg
    15601 tccgtatcga tggccgccac gcgcggcgca atttctcgct gggcagagac gaattttcga
    15661 tatttagcga agtacgcatc catgttggcc tgtggcccct tggtcggatc gagcggaatc
    15721 gacaattcgg gtaggcgctc atcgtaatag tccaccaccg agacggcggt catgcctttc
    15781 ttcagtaagc cgagattggc tttcaacagt tcgccgtatc gtgcgtaggg ctcgtatcgt
    15841 ccagcctgtt cgaggtcacg gcggagccct tccatccgac gcagcagctt tttgagggct
    15901 tttcgtagtg ccgattcgcg ctgtcggacc tgggtgaggt gcgacagttc tgcttcgcgt
    15961 tcccggtaga gaatttccag cctggatgag agtggaaatg gatcagcctc cgtcggaggc
    16021 tgaggttccg gtgcgggagt cgctgtgtct gagggcgatg atcgtaacgg taccgaggcc
    16081 ggtgcctggt agacctggcc cacccgttct ctattgtgac gaagcgtcgc cagcacacga
    16141 gtcgctccat ccagaaataa caggtctgcg tttcgaccga acagttccgc gatcaaggtc
    16201 accggcccat cccggcttgt caggtccagc cgaacgatcc gatcgccggg gatgtgtgtg
    16261 atgccgtcga tacgcgcgcc ttgtatgcgt gcccgcagga gttggcagaa cgagggcggg
    16321 gtcggcgggt tgggaaggct ctcgcgcacg aggtgtatcc gtgccgttcc gtcgcgtaag
    16381 gaacagagca atcggcgagt gtggccgggc acacgaatct ccagcaacag gccgtcaggg
    16441 agcggttggg agatcttctg aatccagccg tcggccaggg ccggcgccag ttcctgaaca
    16501 atggaaccaa tttctgtcgc gctgagcgcc attttcggta cgtgactccc ggtccgcagg
    16561 gatagatcta aaccctgatt cagcaag
//
LOCUS       DAORRQ010000075        16214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_193_length_16214_cov_9.904016, whole genome shotgun sequence.
ACCESSION   DAORRQ010000075 DAORRQ010000000
VERSION     DAORRQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16214
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193_length_16214_cov_9.904016"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK224_17515"
     CDS             complement(<1..357)
                     /locus_tag="PK224_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRB17599.1"
                     /translation="MISTAEFRNGSPLMVEGQPFYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGEKFDTPQIEECDMQFLYATNDSYTFMDTETYEQSTYEKSQLGSNA
                     DLLKENMIAKILIYEHR"
     gene            complement(383..880)
                     /gene="aroQ"
                     /locus_tag="PK224_17520"
     CDS             complement(383..880)
                     /gene="aroQ"
                     /locus_tag="PK224_17520"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRB17600.1"
                     /translation="MLRLLVLHGPNLNLLGTREPSVYGQASLPDIDKSIIRRAGERGI
                     AVQTKQTNVEGELVTWIQNARGHFDGIIINPAAYTHTSIAIRDAIAAVALPTVEVHLS
                     NIHQREEFRHHSFIAGVALGQISGFGPTGYLLALDALAAHLEAVSKASRPRTAPKNKP
                     TASRR"
     gene            complement(1019..1696)
                     /locus_tag="PK224_17525"
     CDS             complement(1019..1696)
                     /locus_tag="PK224_17525"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17601.1"
                     /translation="MASSSRIDPSTAAEIDRLATAVAKDPRSKEFLPLADEYIKVGMW
                     QEAAGVLEDGLKVYPGFVTAMAALGRVYDQLGQAVKAKAILEDVVRQRPDNLRAHRIL
                     AKLYHAEGNADLALLSCTAILNANPFDEEAASVKRSITGAPDNPPAAKREKKRIVTEP
                     RPDGAKAGAHITPAPEPTVSTSSMASEPVTETLGRPTPDLPVVKHAAAIARLEAWLRT
                     IQAQRVH"
     gene            complement(1700..2959)
                     /gene="coaBC"
                     /locus_tag="PK224_17530"
     CDS             complement(1700..2959)
                     /gene="coaBC"
                     /locus_tag="PK224_17530"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRB17602.1"
                     /translation="MQTTGPSLTGVHLVLAVTGSIAAYKAVGLLRLLRREGATVNVVM
                     TAGACRFVTTLTFEVLSGSHVATDLFEAHQEMLHLSLPEQAKAILIAPATANCLAKAA
                     LGLADDLLSTMLLTTQCPVIFAPAMDGDMWQHPTVVEHVATLRTRGAVIVEPEEGPLA
                     SGRSGQGRLADEARILSVLQATLHPRRDWSGRRLLISAGPTQEAIDPVRFISNRSSGK
                     MGYALAEAAQARGAEVLLVSGPTALTPPAGVELCPVTTAEEMRKAVISRFSWSDTVIM
                     AAAVADFRPTQPAAQKLKKRRSPVTSLELSPTDDILRELGERRTTQVLVGFAAETEDL
                     LAHAREKLQAKGVDLLVANDVTAAGSGFGSDTNRVFILDRDGRAEELPLLPKREVADR
                     ILDRVLTLPTGRRSTKPAAGTGTHRKE"
     gene            complement(2969..3331)
                     /gene="rpoZ"
                     /locus_tag="PK224_17535"
     CDS             complement(2969..3331)
                     /gene="rpoZ"
                     /locus_tag="PK224_17535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRB17603.1"
                     /translation="MGEMLSLLPEYATGEFDSRHRLVIIAAQRAKQLVQGARMSGPSK
                     FTKETSIALDEVLRHKVKFLIGQEAREAIKESKRVKEGETERLAMMTGEDAKEIKKEL
                     SVYVDDTVKPAPAPEGEE"
     gene            complement(3324..>4041)
                     /gene="gmk"
                     /locus_tag="PK224_17540"
     CDS             complement(3324..>4041)
                     /gene="gmk"
                     /locus_tag="PK224_17540"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRB17604.1"
                     /translation="TERAPVSSNDKPPQVRRGILFIISAPSGSGKTTLCKQITANVPG
                     LWHSISYTTRKPRPGEVDGQDYHFLDEPAFRQMIDRNEFVEWAHVYGNLYGTPRKMLT
                     EKMEQGIDVLLEIDVQGARSVKKKFEDGVYIFILPPSFDTLRTRLQNRAGDSPDEIQR
                     RLQKAKEEVWSYREYYYIVRNDDLKQSLKELESIFLAERIKTKRLNMTWLEEKFILDK
                     EGKQGNSSSAPASKEHLHHG"
     assembly_gap    4042..4051
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4052..>4674)
                     /locus_tag="PK224_17545"
     CDS             complement(<4052..>4674)
                     /locus_tag="PK224_17545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRB17605.1"
                     /translation="ALAKQYHTALKKLQKTLGLRGAVDVSTLAGFRDILSISDEPVDA
                     GQLTKTVLRVLGGALTDLEKMRRREGEALAKDLALHLDAIREAKSLVAEKAPVLAKHA
                     FERMKGRIETLLQAELPDPARLQQELAVFADRSDISEELVRLESHMLQFDQQLHSKES
                     VGKTLEFLLQEMGREVNTIGSKANDADIAALVVRMKAELEKLREQVQ"
     assembly_gap    4675..4720
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4721..4934)
                     /locus_tag="PK224_17550"
     CDS             complement(<4721..4934)
                     /locus_tag="PK224_17550"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17606.1"
                     /translation="MIRSMTGYGKKDGTSQKAGVTVEIRSVNHRFLEVAVRLPRSLAQ
                     LEDQVRKTVQQRCLRGRVDVSVSVHSA"
     gene            complement(4931..5602)
                     /gene="nth"
                     /locus_tag="PK224_17555"
     CDS             complement(4931..5602)
                     /gene="nth"
                     /locus_tag="PK224_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRB17607.1"
                     /translation="MKVATTDQPAGVTTKRRLARMLTALRATSPAMKVELDHRTPWEL
                     LVATILSAQCTDQRVNQVTPNLFRRYQQPRDYASADPTELEALIRPTGFFKTKARNLI
                     HCAKAVAGQFQGEVPDTMEALTTLPGVGRKTANVLLGNAFEKPAIVVDTHVKRVAGRL
                     DLTRHTDPEKIELDLQRLLPADQWTEGSQRLLLHGRYICLARTPKCQHCPIYADCHWK
                     GKTAR"
     gene            complement(5636..7405)
                     /gene="hflX"
                     /locus_tag="PK224_17560"
     CDS             complement(5636..7405)
                     /gene="hflX"
                     /locus_tag="PK224_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRB17608.1"
                     /translation="MRYRHRRAAKRCVADPNVRVPRSITIPAIHGHVTGLKASHLAAL
                     ERLYRRRVPSSAVLTTELARTTSQLTREIRRPIGLLITRRGVIEQVLVGTGCAPTFES
                     LAKFRVGSHSLRGLRLIRTHLHDDPLSQDDLTHLALLRLDLIGILGVDEKGDPSLLHL
                     AHLLPPNQQGEVCRILKPVPFHDLDLQLDTFLHALESDLQRSDTKHDVAAGTESAILV
                     SAAPKSHAEQEEHLEELNELAESAGVRVLDRIAQRTHEGYERYLLGKGKLKEVVMRAL
                     QKGADLVIFDQDLAPAQARAISEVTDLKVIDRTQLILDIFAGRAHTREGKVQVELAQL
                     RYLLPRLSGHGTSLSRLGGGIGSRGPGETKLETDRRRIRDRIAHLEREIDDVARHQDQ
                     RRSRRVRQGLPILSIVGYTNAGKSTLLNTLTHSQIPAQDRLFETLDTTSRRLRFPHDR
                     EVIVTDTVGFIRDLPKDLVGAFRTTLDELRDADILLHVVDASAPNVDQQITAVETVLQ
                     SLNLDTIPRVMVLNKCDRLSAHEAGVLCQRYHAIGISAPNRETLRPLIAHLESLLPAI
                     PTPPGYEEDPDQPPQDLALASHS"
     gene            complement(7356..8414)
                     /gene="tsaD"
                     /locus_tag="PK224_17565"
     CDS             complement(7356..8414)
                     /gene="tsaD"
                     /locus_tag="PK224_17565"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRB17609.1"
                     /translation="MKPPASTTILGIETSCDETAAAVLDGEGHVLANVISSQQTVHER
                     FGGVVPELASRAHIQNVDHVTRQALDAAGLTWQDLGAIAVTQGPGLAGALLVGISYAK
                     SLAYALGIPLVGVSHLEGHIASAWIDRPSFPRTCVVLIASGGHTHLFHATEHGGFHLM
                     GRTIDDAAGEAFDKGAQMLGLGYPGGPLIDAAARKGSPAAVRFPRSRVRTGQLDFSFS
                     GLKTALLYHLQRMDQEAIVRQQADLAAGYQEAIVDVLVRQTFAAVRQAGVSALAVVGG
                     VSANSRLRARLRERAAGEGIELGLPALSYCTDNAAMIAAAGQRALEQGRLASFECEAM
                     PNYALPASSGGETMRRRS"
     gene            complement(8663..11092)
                     /locus_tag="PK224_17570"
     CDS             complement(8663..11092)
                     /locus_tag="PK224_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRB17610.1"
                     /translation="MFERFTDKGRKIIILAREEAERHQNDYLGTEHLVLAILRETDGI
                     ALMILKKMGLSTEQIRLEIERNLPGGGTTMTFGEIPFSPRVKKVIEYGVEEARLLGHN
                     HIGSEHLLLGLLREEEGIGGKILRSLGANLLTARQLTVTFLRKSAPRERDRKSNTPAL
                     DEFGRDLTQLAQEGQLDPVIGRADEIERVLQILSRRSKNNPVLIGESGVGKTAIVEGL
                     AQRIIASEVPDNLLSRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVQAGNIIIFID
                     ELHTLVGAGAAEGSIDASNMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPI
                     HVQPPSTDETVRIIQGLRDRYEEHHGVEITEDAIVEAVKLADRYITDRFLPDKAIDLI
                     DETGSRAKLQTYALPTELKALEQELKKISRDKELAISMQNFEEAVRHREEEERLRKLL
                     DESKREWKKNQEKHKPTIGKEEVAYVVSKMTGIPLFKLEEEESNKLLRMEEFLHKRVI
                     GQNEAISAVARAIRRSRAGLKEAKKPIGSFIFLGPTGVGKTELARTLAEFLFNSEDAL
                     IRIDMSEYQEKFTSSRLFGAPPGYVGYEEGGQLTEKVRRRPYSVVLFDEIEKAHPDVF
                     NVLLQVLDDGVLTDSLGRKVDFKNTVVIMTSNIGTKMIQKGVSLGFQNDEKGGQALRR
                     KEDVMGELKRSFSPEFLNRIDEIVIFHSLEKEHLIHILDILLHELNLRLIDKNVSIEV
                     DDEVKQWLIKEGYEPLYGARPMRRIIQRAIGDPLSEELIKGRFKENRKIKVVLRDGAP
                     AFIEQEAMAGV"
     gene            11379..12239
                     /locus_tag="PK224_17575"
     CDS             11379..12239
                     /locus_tag="PK224_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRB17611.1"
                     /translation="MKSKSIGILTKPKFPEVKSTVQAVVSWLRSRNIDVLLDTTATTL
                     LGEQGGFQKTHLASKADVLLVLGGDGTMLNAARLAGERGIPILGVNMGGLGFLTEVVL
                     DNLYPSLERMFANDFVLDERLMLKTHVHRHGETVARGVVLNDVVISKGTLARMIELKI
                     AIQGQFVTNLRGDGLIISTPTGSTAYSLSAGGPIINPAVPSLILTPICPHTLTHRPLI
                     VPASAEIEVVLTSKDDGAMATLDGQVGVALTQGDTVEIRASEHMTRLIRFPESSYYEV
                     LREKLKWGDG"
     gene            complement(12261..13631)
                     /locus_tag="PK224_17580"
     CDS             complement(12261..13631)
                     /locus_tag="PK224_17580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB17612.1"
                     /translation="MSATGSETSGHVVIVVGAGPAGMALAKKMADAGHEVIILNRDIK
                     FGGLAEYGIFPSKLKLRGGLKKQYWDMLEQPNVHYFGNVSVGQGKDLTVEDLRGLGAS
                     AVAFSIGAQGTKAIGVEGDSAKGVFHAKDVVYHFNRLPGFGDRPFDMGKHVAIIGVGD
                     VMVDIAHWLIKYKKVERVTAIARRGPVERKYNPKEIRAVCSNMDQEGIATEFARIKDR
                     LAAVGQNADEVLASMTAEFTKCEPTGSPSKMGFRFLASPKRVLVDANNRVRALEMEEN
                     KLEPKGEDTAAVGLKQLYEFPVDSVVFAVGDRVDETVGLPYKNGVYVTNPNKTGNDPD
                     DSLFQAYDEKSGQVVDGVFLAGWARKASEGLVGIAKRDGDWCAEVISRYLTTQATRSR
                     GTMDGVIAKLHTILKDRKSRPVDLEGLKVLDTVEKAHAASPEAIGEFKFASNADMLAH
                     IERGRG"
     gene            complement(13814..15268)
                     /locus_tag="PK224_17585"
     CDS             complement(13814..15268)
                     /locus_tag="PK224_17585"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRB17613.1"
                     /translation="MKKASRTTGTAASMTTSTRMERDTMGELPVPSNAYYGVQTARAI
                     ENFPISSLRIPRAMIRAMGLIKRAAASVNHSLKLLDKKPADAIVRAATEVVEGRLDAE
                     FPVDIFQTGSGTSTNMNTNEVISNRATELLGGARGSKLVHPNDHVNPGQSSNDVVPTA
                     IHIAASETIQRQLIPALTQLHKALNGKAREFDKIVKIGRTHLQDATPVRLGQEFGGYA
                     RQIELGIARMKRAQAALSEVALGGTAVGTGLNCHPKFAGKVMAIISKETGCAFKEAVN
                     HFEGQSAQDSLVEASGDLRTLAVSLMKIANDIRWLGSGPRCGLGEINLPETQPGSSIM
                     PGKVNPVIAESVTMVCAQVIGNDVTITVGGQAANFELIVMLPVMAYNLLQSIELLATA
                     STNFAVKCIDGIKANEERCKSLIEESLAMCTALAPVIGYEAAAKLAKDAYKSGKTVRE
                     VAREQNVLPEKRLTQLLDPWRMTQAGGPVGSAGG"
     gene            complement(15500..15955)
                     /locus_tag="PK224_17590"
     CDS             complement(15500..15955)
                     /locus_tag="PK224_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRB17614.1"
                     /translation="MAIVRWDPFRELEEMSDRLNRMIARPSTGTASGQGKEVMTVADW
                     TPTVDISETESEYAIKAELPEVKKEDVKVTVEDAVLTIQGERKQEKEEKGKKYHRIER
                     SYGRFVRSFTLPDSVDESKVRAEYADGVLHLHLPKSEKAKPKQIDVKIS"
BASE COUNT         2956 a         4691 c         4873 g         3638 t
ORIGIN      
        1 ccggtgctcg tagatcagga tcttggcgat catgttttct ttgagcagat cggcgttgct
       61 tcccaactgg ctcttctcat aggtcgactg ctcatacgtc tccgtatcca tgaacgtgta
      121 ggaatcgttc gtggcataca ggaattgcat gtcgcattct tcgatctggg gtgtgtcgaa
      181 tttttctccg gaacgaaagg tcctgtcgag gacgttgcct gacagataac tcttcagctt
      241 cgtccggacg aatgccccgc ccttgccggg tttgacgtgc tggaattcca cgatataaaa
      301 gggttgacct tctaccatta acgggctgcc gttccgaaac tctgcggtcg aaatcacaac
      361 aactctcctt ggtacaacaa gttcaacggc gcgaagccgt gggtttattc ttcggggccg
      421 tccgagggcg tgatgccttg ctgacggcct cgaggtgcgc ggccaatgcg tccagcgcca
      481 gcagatagcc ggtgggaccg aatccggaaa tctgtcccag ggcgacgccg gcgatgaagg
      541 agtgatggcg aaactcctcc cgctgatgga tgttcgacaa atgcacctcg accgtcggca
      601 gggcgacggc ggcgatcgca tcgcgaatcg cgatgctggt atgggtatac gcggccggat
      661 tgatgatgat gccgtcgaaa tggcctctgg cgttctgaat ccaggtgacc agttcccctt
      721 ctacattcgt ctgtttggtc tgcaccgcaa tcccccgctc gcctgcgcga cggataatcg
      781 acttgtcgat gtcgggcaag gacgcctgtc cgtagacgga tggctctcgc gttcccagga
      841 gattgagatt gggaccatga agcactagca gccgcagcat gtgtctgtcg cgcgcccccg
      901 ttatcagtag gctgcaatgg atagagatgc cagcagcggc ggcattctaa cagggtgtca
      961 ggagtgggtc aaacgttgtt cagagggcgg gacgagcgtg ggtccgcctg gggggcagtc
     1021 agtgaacgcg ttgggcttga atggttcgca gccaggcttc tagtcgcgcg atcgcggccg
     1081 catgtttcac gacaggcaga tccggggtcg gccggcccag tgtttcagtg accggctcag
     1141 atgccataga tgacgtggat acggttggct cgggagcggg cgtgatgtga gccccagctt
     1201 ttgcgccgtc gggtctaggc tccgtgacga ttcgtttctt ctcgcgtttg gcggccggag
     1261 gattgtcagg tgctccggtg atgctgcgtt tgactgaggc cgcctcttca tcgaacgggt
     1321 tggcgttcag gatggctgtg caggacagca acgctaagtc ggcgttccct tcggcgtgat
     1381 agagcttggc gagaatacga tgcgcgcgaa gattgtccgg gcgctgtctg acgacgtctt
     1441 caagaatggc cttcgctttc accgcctgcc ctaattgatc gtacacccgg cccaatgccg
     1501 ccatggcggt gacaaagccg ggatacacct tgaggccgtc ttcaagtacg cccgctgctt
     1561 cctgccacat gccgaccttg atgtattcgt ctgcgagggg gaggaattct ttcgagcgcg
     1621 ggtcttttgc gacggcggtg gccaggcggt cgatttcggc ggcggtcgat ggatcgatac
     1681 gcgatgaaga agccacgccc tactccttgc gatgtgtccc tgttcctgcg gctggcttcg
     1741 tcgaccttcg gccggtcggt agggtcagga cccggtccaa aatgcggtct gcgacctcac
     1801 gcttgggtaa cagggggagt tcctccgcgc ggccgtcacg atccaggatg aacacgcgat
     1861 tcgtgtccga cccgaagccc gatcctgctg ccgtcacgtc attggcgacc agcaggtcga
     1921 cgcccttagc ttgcagcttt tcccgcgcat gggcaagcag atcctcagtt tccgccgcaa
     1981 accccacgag cacctgtgtc gtccggcgct cgcccaattc cctaagaata tcgtcggtcg
     2041 gcgacaactc caggctggtg acgggactcc gtcgcttttt gagtttctgt gcagcgggtt
     2101 gtgtcgggcg aaagtccgcc acggcggccg ccatgatgac ggtatcgctc caggaaaacc
     2161 ggctgatgac agccttgcgc atctcctcag ccgttgtcac tggacacaac tccacgccgg
     2221 ccggcggggt cagggccgtg gggcctgaga ccagtaggac ctccgcgccg cgtgcctgcg
     2281 cggcttcggc gagcgcatat cccatctttc cagaggaccg gttggaaatg aaccgcacgg
     2341 ggtcgatggc ttcctgcgta gggccggcgg aaatgagcag acggcgaccg gaccaatccc
     2401 gacgcgggtg gagcgtggcc tggagtaccg aaaggattcg tgcttcatcg gccagccgtc
     2461 cctgccctga acggcctgag gccagcgggc cctcttctgg ttcgacgatg actgcgccgc
     2521 gtgttcgcag cgttgccacg tgctccacga cggtcgggtg ttgccacata tccccgtcca
     2581 tggccggcgc gaaaatcacg ggacattgtg tagtgaggag catggtcgag aggagatcgt
     2641 cggcaagccc gagcgcggct ttggccagac aattggctgt agccggggca atcaggatag
     2701 ccttcgcctg ctccggcagg gagaggtgca gcatttcctg gtgggcttcg aagaggtcgg
     2761 tggccacgtg ggaaccggac aacacttcga aggtcagcgt ggtcacgaac cggcaggccc
     2821 ctgccgtcat gacgacgttc accgtcgcgc cttcgcgacg gagcaggcgc aagagcccga
     2881 ctgccttata ggcggctata ctccccgtga cggcgagcac aaggtggaca ccggtgagtg
     2941 aggggccggt ggtctgcacg aagcgggact actcctcgcc ctccggagca ggggccggct
     3001 tgacggtatc gtcgacgtag acgctcagct ctttcttgat ttccttggcg tcctctccgg
     3061 tcatcatggc caggcgttcc gtctcacctt ccttgacccg tttggattct ttaatggcct
     3121 cgcgggcttc ctgtccgatc aggaatttga ccttgtgccg aaggacttcg tcgagcgcga
     3181 tgctcgtttc tttggtgaat ttggacgggc cggacatgcg cgcgccctgc acgagttgtt
     3241 tcgcccgctg cgcggcgata atcaccagcc ggtggcgtga atcgaactcc ccggtggcgt
     3301 attccggtaa caaacttaac atttcaccca tgatgcaagt gctcctttga tgcgggggcg
     3361 gaggaggaat tcccctgctt gccctctttg tcgagaatga atttttcttc caaccacgtc
     3421 atgttcaggc gtttggtttt gatccgttcc gccaggaaaa tgctttcgag ctctttcaag
     3481 gattgcttca ggtcgtcgtt ccggacgatg tagtagtact cccggtagct ccagacctct
     3541 tctttggctt tctgcaggcg gcgttgaatt tcgtccggcg agtccccagc ccggttttga
     3601 agccttgtcc gcagcgtgtc gaacgacggc ggcagaatga agatatacac gccgtcttca
     3661 aacttttttt tcactgagcg cgcgccctgc acatcgattt cgagcagcac gtcgatgccc
     3721 tgttccattt tctcggtcag catcttgcgc ggtgtgccgt acaaattgcc gtagacatgg
     3781 gcccattcga cgaattcatt ccgatcgatc atctggcgga aggccggctc atcgagaaaa
     3841 tggtaatcct gcccatccac ctcgcccggg cgcggtttgc gtgtggtgta cgagatcgaa
     3901 tgccacaatc cagggacatt ggccgtgatc tgtttgcaca atgtcgtctt cccgctcccc
     3961 gacggagccg aaatgataaa caaaatgcct cgccgtactt ggggcggttt atcgttggat
     4021 gaaacgggcg cgcgctcagt gnnnnnnnnn nttctgaact tgttcgcgca atttttccag
     4081 ttcggctttc attcggacca ccaacgcggc gatgtcggcg tcgttggcct ttgagccgat
     4141 ggtgttcact tcccgcccca tttcctggag caggaactct aaggtcttcc ccaccgactc
     4201 cttactgtgg agctgctggt cgaactgtag catatgtgac tcaagtctga ccaattcttc
     4261 tgagatgtcc gaacggtcgg caaacaccgc taattcctgt tggagccggg ccggatccgg
     4321 caattcggcc tgcagcaggg tctcaatccg gcctttcatg cgctcaaatg catgcttggc
     4381 cagtaccggg gccttttccg ccacgaggga cttggcctca cggatcgcat ccagatggag
     4441 agccaggtct ttggcgagtg cctccccttc ccgtcggcgc atcttctcaa ggtctgtgag
     4501 cgcgcctccc agtacccgca gcacggtttt cgtcagttgc ccggcatcga ccggctcatc
     4561 ggaaatagac aggatgtccc ggaatcccgc gagcgtggaa acatccaccg ctccccgcag
     4621 ccccagtgtt ttctgcagct tcttgagggc cgtatgatac tgtttggcca acgcnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggcggaatg gaccgagacc
     4741 gacacgtcga ctcgtccgcg gaggcagcgt tgctggacgg tttttcgaac ctggtcctcc
     4801 agttgcgcca acgaccgggg cagtctgact gccacttcga gaaagcggtg attgaccgaa
     4861 cggatctcca cggtgacgcc ggctttctgt gacgtgccgt cctttttccc ataccccgtc
     4921 atgcttcgaa tcatcgtgcg gttttcccct tccaatggca atcggcatag atcggacaat
     4981 gctgacattt cggtgtgcgc gcgaggcaga tgtatcggcc atgcagaagg agccgttggg
     5041 atccttccgt ccactggtct gccggcagta agcgttgcag gtccagctca atcttttcgg
     5101 gatccgtatg gcgggtcagg tcgagccgtc cggccactcg tttcacatgt gtgtcgacca
     5161 cgatggccgg cttctcgaat gcgttgccaa gaaggacatt cgccgtcttt cgcccgactc
     5221 ccggtagcgt ggtgagtgct tccatcgtat cgggaacctc tccctggaac tgcccggcca
     5281 cggccttcgc acagtggatg aggttcctgg cttttgtttt gaagaagccg gtcggccgga
     5341 tgagggcttc caattccgtg ggatcggccg aagcgtaatc gcgaggctgc tgatagcgac
     5401 ggaacagatt cggggtgacc tgattcaccc gctgatcggt gcattgcgcc gacaggatcg
     5461 tggcgaccag taactcccag ggcgtgcgat ggtcgagctc gactttcatc gccggtgagg
     5521 tcgcgcgtaa tgcggtcaac atccgggcga ggcggcgttt ggttgtcacg ccggccggtt
     5581 gatctgttgt ggcgactttc atgatgcggt gtggtggcgg ccaattgtgc agaggtcagg
     5641 agtgagatgc aagcgccagg tcctgaggcg gctggtcagg atcttcctcg tatcccgggg
     5701 gtgtcggaat ggcgggcaag agggattcaa ggtgggcgat caacgggcgc agcgtctcac
     5761 ggttcggcgc cgagataccg atcgcgtgat accgctggca gaggacgccg gcctcatgtg
     5821 ccgagaggcg atcacacttg ttcaggacca tgacgcgggg aatcgtgtcg aggttgagcg
     5881 attgcaagac ggtttcgacg gcggtgatct gttgatcgac gttgggtgcc gacgcatcga
     5941 ccacatgcag gagtatgtcg gcatcccgca attcgtccag cgtggtgcga aaggctccca
     6001 ccagatcttt ggggagatcc cggatgaacc ccaccgtatc ggtcacgatg acttcgcggt
     6061 catgaggaaa acgaaggcgg cggctcgtcg tgtcgagggt ttcaaagaga cgatcctggg
     6121 ccgggatctg actgtgggtc agggtgttga gcagcgtcga tttgcctgca ttggtatagc
     6181 cgacgatgga gaggatcgga agcccctggc gcacacgtcg ggatcggcgc tgatcttgat
     6241 ggcgggccac gtcgtcgatc tcccgctcca ggtgcgcgat gcgatcacgg atgcggcggc
     6301 gatcggtttc cagtttggtc tcaccggggc cgcgagaacc gattccgcct ccaaggcgcg
     6361 acaacgaggt gccatgcccg gacagtcgcg gcagaagata tcgaagctga gccagctcca
     6421 cttgaacttt gccttcacgg gtatgggcgc gcccggcgaa aatatccaga atgagctgcg
     6481 tgcgatcgat cactttcaga tccgtcacct cggaaatggc gcgggcttgc gccggggcga
     6541 gatcctggtc gaagatgacg aggtcggctc ccttctggag cgcgcgcatc acaacctctt
     6601 tcagtttgcc cttcccgagc aagtatcgct cgtacccttc gtgagtgcgc tgggcgattc
     6661 gatccagcac gcggaccccg gcggactcag ccaattcatt cagctcctct agatgctctt
     6721 cttgttccgc gtgactcttg ggtgccgcgc tgacaagaat ggccgattcg gtgccggcgg
     6781 cgacgtcgtg cttggtatcc gatcgctgca aatcgctttc gagcgcatgg aggaatgtgt
     6841 ccagttgaag gtccagatcg tggaacggga ccggtttcag gatgcggcag acttctccct
     6901 gttggttggg tgggagcaga tgcgccagat gcagcaggct ggggtcgcct ttttcgtcta
     6961 cgcccaggat cccgatcaga tccaatcgca ggagcgccag gtgggtcaaa tcgtcctggc
     7021 tcaacgggtc atcgtggaga tgcgtgcgaa tcagccggag accgcggagg gaatgtgatc
     7081 caactctgaa cttcgccagc gactcgaagg tgggtgcgca gccggttccc acgagcacct
     7141 gttcgatgac cccgcggcga gtgatcagga gaccgatggg acggcgaatc tcacgtgtca
     7201 actggctggt cgtgcgcgcc agttcggtcg tgaggacggc tgaggacggc acacggcgtc
     7261 gatacaatcg ctccaatgcc gcgaggtgac tggctttcag cccggtgacg tgtccgtgaa
     7321 tcgcggggat ggtgatgctc cttggtactc gaacgttagg atctgcgacg catcgtttcg
     7381 ccgcccgacg atgccggtaa cgcataattc ggcatcgcct cgcactcgaa ggatgccagc
     7441 ctgccctgct ccagagcccg ttgccctgcc gccgcaatca tggccgcatt gtctgtgcag
     7501 tatgagaggg ccgggagtcc caattcaatc ccctccccag ctgcccgctc cctcaatcgt
     7561 gctcgcagcc tggaattggc cgagacgccg ccgacgacgg cgagggctga gacgcctgcc
     7621 tgtcgcacgg ccgcgaatgt ctgacggacc aacacgtcca cgatcgcttc ctggtacccg
     7681 gcggccaggt cggcctgttg tcggacgatg gcttcctgat ccatgcgctg gaggtgatag
     7741 agcagggccg tcttgagacc gctgaaactg aaatccagtt gtccggttct cacgcgcgat
     7801 cgaggaaatc gcacggctgc cggactgcct tttctggccg cggcgtcgat gagcggtcca
     7861 cctgggtatc cgagcccgag catttgtgcg cccttgtcga atgcttcgcc tgcagcatcg
     7921 tcgatcgttc gtcccatcag gtggaagccg ccatgttccg ttgcgtggaa gagatgcgtg
     7981 tgcccgcctg aggcgatcaa cactacgcag gttctgggga atgacgggcg gtcgatccag
     8041 gctgaggcga tgtggccttc gaggtgactc acgccgacca gtggaatgcc aagcgcgtag
     8101 gctaaggact tggcataact gatacccacc agcagggcgc cggccaatcc gggaccctgg
     8161 gtgacggcga tggcgccgag atcctgccag gtgagacctg ctgcgtccag agcctgacgt
     8221 gtgacgtgat ccacattctg aatatgagca cgtgaggcca attccggcac taccccgccg
     8281 aaccgttcgt ggacggtttg ctgggaagaa atcacgttgg ccaggacatg cccttcaccg
     8341 tcgagcaccg ctgcggcagt ctcgtcgcag gatgtttcga tgccgagaat cgtcgtggat
     8401 gcaggaggct tcatagagaa aactgccgac agggtgacgg catgtgaacc tggttatgca
     8461 tgcgcgcgcc attccaaggg gcgagatcag tcgtagtggg cgcacactgt gactggtgct
     8521 gttcgtaagg aaatccggac aacacctgat cctgccgtaa aatgcgacaa cccccggatc
     8581 cggttgcgga ccgcgggggt tgccgggtgg tctctaggat ctcaacgacg agtcgatcct
     8641 ctcgtaatcg tcttcgctcg cgctagacgc ctgccatcgc ctcttgttcg atgaaggccg
     8701 gcgcaccgtc gcgcagcacg actttgatct tcctgttttc cttgaagcga cccttgatga
     8761 gttcttcaga caggggatcg ccgatcgcgc gctgaatgat gcgccgcatc ggacgcgccc
     8821 cgtagagcgg ctcgtatcct tccttgatga gccactgctt gacctcgtcg tctacctcga
     8881 tgctgacgtt cttatcgatg aggcgaagat tcagttcgtg cagcaggata tccaggatgt
     8941 ggatcaggtg ctccttctcc agggaatgga agatcacaat ctcatcgatc cggttcaaaa
     9001 actccgggct gaacgaacgc ttgagttctc ccatcacgtc ttcttttcgg cgcaaggcct
     9061 gtccgccctt ttcatcattc tggaatccga gcgagacgcc tttttggatc atcttcgtgc
     9121 cgatgttcga ggtcatgatg acgaccgtgt tcttgaaatc gacttttcga cccaggctgt
     9181 ccgtgaggac accgtcgtcg agcacctgca gcaggacgtt aaacacgtcc ggatgcgctt
     9241 tctcgatttc gtcgaacagc acgaccgaat agggacgacg tcgaaccttt tccgtgagct
     9301 gtccgccttc ctcgtaaccg acgtatcccg gaggggctcc gaagaggcgt gaactcgtga
     9361 acttttcctg atactcggac atatcgatcc ggatgagcgc gtcttcgctg ttgaagagga
     9421 actccgccag cgtcctcgcc aattccgtct ttccgacacc ggtcgggccg aggaaaatga
     9481 aggacccgat gggtttcttc gcttccttga gacccgctct ggatcgacga atggcacgcg
     9541 ccactgccga aatagcctcg ttctgtccga tgacccgctt gtggaggaat tcttccatgc
     9601 ggagcagttt attggattct tcttcttcca acttgaagag cggaatgccg gtcatcttcg
     9661 acacgacata cgcgacctct tccttgccga tggtcggctt gtgtttctcc tggttcttct
     9721 tccactcccg cttggattca tcgaggagct ttcgcaggcg ctcctcttct tcgcgatggc
     9781 ggacggcttc ctcgaagttt tgcatcgaga tcgccagctc tttatcgcga gaaattttct
     9841 tgagctcctg ctccagcgct ttgagttcag tcgggagcgc gtacgtttgc aacttcgccc
     9901 gcgagccggt ttcgtcgatc agatcgatcg ccttgtccgg caggaaccgg tcggtaatat
     9961 agcgatccgc caacttgacg gcttccacga tggcgtcttc cgtaatttcc accccatggt
    10021 gttcctcgta ccggtcccgc aagccctgga tgatccgcac cgtttcatcc gtgctggggg
    10081 gctgtacgtg gatgggttgg aaccgacgtt tgagcgcgcc gtccttctcg atgtgtttac
    10141 gatattcgtc gagcgtcgtg gcgccgatgc attgaatctc gccgcgggac aacgccggct
    10201 tgagcatgtt ggaggcatcg atggatcctt ctgccgcccc tgccccaacc aacgtgtgca
    10261 actcgtcgat gaagataatg atattcccgg cctgcacaat ctccttcatc acgaccttga
    10321 ggcgctcttc aaactggccg cggtatttcg tgccggccac cagtgagccc aggtcgagcg
    10381 cgatgacgcg acgggacagc agattgtccg ggacttccga cgcgatgata cgctgggcga
    10441 gtccctcgac gatggctgtt ttccccaccc ccgattcacc gatgagtacg ggattgtttt
    10501 tcgaccgtcg gctgagaatc tgcaggacac gctcgatttc gtccgcgcgg ccgatgacgg
    10561 gatccagctg cccctcctgg gccagctggg tcaggtcgcg tccgaattcg tcgagtgccg
    10621 gcgtattgct cttgcggtcc cgctcacgcg gcgcagactt acgcaggaag gtgacggtga
    10681 gttgccgggc ggtcaggagg ttggcgccaa gactccggag aatcttcccg ccgatgcctt
    10741 cttcctcacg aaggagaccg agcaacagat gttcgctgcc gatgtggtta tgaccgagca
    10801 accgggcttc ttcaaccccg tactcgatga ctttcttgac ccgcgggctg aaaggaattt
    10861 ccccgaaggt catcgttgtg cccccgcccg ggagattccg ctcgatttcg agtcgaatct
    10921 gctcggtgga gagccccatt ttcttgagga tcatcagcgc aatcccatcc gtctcgcgaa
    10981 ggatggccaa caccaggtgc tcggtgccca ggtaatcgtt ctgatggcgc tcggcttctt
    11041 cacgtgccag gatgatgatc ttgcgaccct tgtccgtgaa ccgttcgaac atcccgcctc
    11101 cttatggtga tggggtctgt aagagaagcc ttagctgagt gattcaatgc aggtcaaagg
    11161 ctcgaaaata ctgatgatgt gattctaact agcgcaatat ggggtgtcaa ggtattgagc
    11221 gaaacgcgag agccgcgcgg cgactgttga aacagcggcg tgcgcggcgg aggaaatctc
    11281 attcttgctg cgcgctcgtt tgacagtaaa agaatcatct cgatacaatc ccgcaccttt
    11341 caattggctt ctcatctcct cgcgacgcgg tctcatgtat gaaaagtaag agcatcggca
    11401 tcctgacgaa accaaaattt ccggaagtga aaagcacggt gcaagccgtg gtctcgtggc
    11461 ttcgttcccg gaacatcgac gttctcctgg atacgaccgc gacgacgctg cttggcgagc
    11521 aaggcgggtt tcaaaaaacg catctcgcca gcaaggccga tgttttgctc gtgttgggag
    11581 gcgacggcac gatgctcaat gcggcgcgat tggccggcga gcgcggcatt ccgattctcg
    11641 gtgtgaatat gggcggtctg ggattcttga ccgaagtcgt cttggacaat ttgtatccgt
    11701 cgctcgagcg gatgttcgcg aacgatttcg tgctcgacga gcgactgatg ctgaagacgc
    11761 atgttcatcg gcacggcgaa acggtcgccc gcggcgtggt cttaaacgat gtcgtcatca
    11821 gtaagggcac gctcgcccgg atgattgaac tcaagatcgc aattcagggg cagtttgtga
    11881 cgaacctgcg cggggacgga ctgatcatca gcaccccgac cggatcgact gcgtactcgc
    11941 tttcagccgg cggtcctatc atcaatccgg cggtcccgtc attgattctg acgccgatct
    12001 gcccgcacac gctgacacat cgaccgctga tcgtgcctgc cagcgcagaa attgaagtgg
    12061 tcctgactag taaagatgat ggggcgatgg cgacgctcga cggccaggtc ggggtggcat
    12121 tgacccaggg cgacacggtg gagatccggg cctccgagca catgaccaga ctgatccgtt
    12181 tccctgagag tagctattat gaggtgttgc gagagaagct gaaatggggc gacgggtaag
    12241 cgaggctgag tggggcgcgg ctagccgcgc ccccgttcga tgtgggccag catgtcggcg
    12301 ttcgatgcaa acttaaactc tccgatggcc tcgggagagg ccgcatgagc tttttccacc
    12361 gtatccaaca ctttgagtcc ttcgagatcc accggccgac tctttcgatc cttgaggatg
    12421 gtgtgaagtt tcgcgatcac tccatccatc gttcctcgtg agcgcgtcgc ttgagtcgtc
    12481 aagtagcgtg agatcacttc cgcgcaccag tccccgtccc gcttggcgat ccccaccaga
    12541 ccttcgctgg ctttgcgggc ccaaccggcg agaaaaacgc catcgaccac ctgccctgat
    12601 ttctcgtcat aggcttggaa gagggaatcg tccggatcgt ttccggtctt gttcgggttg
    12661 gtgacgtaga cgccgttctt ataggggagc cccacggttt catcaacccg gtcgcccacg
    12721 gcaaacacga cgctatcgac ggggaactca tacaactgtt tgagtccgac cgcggcggtg
    12781 tcttcacctt tcggctccag cttattttct tccatctcaa gtgcgcggac gcgattgttg
    12841 gcgtcgacca gtacgcgctt cggggaggcc agaaaacgaa agcccatctt ggaggggctg
    12901 cctgtgggtt cacacttcgt aaattcggcg gtcatactcg ccagcacttc gtcggcattc
    12961 tgaccgactg cagccagccg gtccttgatg cgggcaaatt ccgtggcgat gccttcctga
    13021 tccatgttgg agcagacggc ccggatttct ttcgggttgt atttgcgctc aaccggcccg
    13081 cggcgggcga tggcggtgac acgctccacc ttcttatact tgatcaacca gtgggcgatg
    13141 tcgaccatca cgtcgccgac gccgatgatc gcgacatgtt ttcccatgtc gaacggacgg
    13201 tcgccgaaac ccggcaggcg attgaagtgg tacactacgt ccttggcatg gaacacgccc
    13261 ttggcggaat ccccttcaac cccgatggct tttgtccctt gcgcgccgat ggagaaggcg
    13321 acggcgctgg cacccaatcc gcgcaaatct tcaaccgtga ggtccttacc ctgcccgact
    13381 gagacgttgc cgaagtagtg gacattgggc tgctcgagca tgtcccaata ttgcttcttc
    13441 aacccaccac gcagtttgag cttgctgggg aagattccgt actccgccaa cccgccgaac
    13501 ttaatgtcgc gattgagaat gatgacctcg tggcccgcat ccgccatctt cttggccagg
    13561 gccattccag ccgggcctgc gcccacgacg atgacgacat gtccgcttgt ctcagaaccg
    13621 gttgcgctca ttatctaccc ttcaatctat atcgaaagtg aatcattaat gacgaagacc
    13681 gtcgaacggt cgaaagttta aaaattttgc gcacgttagc acagggttcc ttgggctgtc
    13741 aaaatcgccg accgtcccgg tgaaccccgg tgtggtcgga ttatcacgcc cggtgagccg
    13801 gtctggtggt cgtctacccg cctgcactgc cgaccggtcc gccggcctgc gtcatacgcc
    13861 acggatccag gagttgcgtg agtcgctttt ccggcaacac gttttgttcg cgagccactt
    13921 cccggacggt cttgccggac ttataggcat ccttggcgag ttttgcggcc gcctcgtagc
    13981 cgatcaccgg cgcgagcgcg gtgcacatgg cgagactttc ttcgatgaga ctcttgcagc
    14041 gttcctcgtt ggccttgatc ccatcaatgc atttcactgc aaagttcgtg gaggccgtcg
    14101 ccagcagctc gatggactgc agcaggttgt aggccatcac cggcagcatg acgattaatt
    14161 cgaaattggc cgcctggccg ccgacggtga tcgtgacgtc attgccgatc acttgggcgc
    14221 agaccatggt gacggattcc gcaatgaccg gatttacttt gcccggcatg atggatgagc
    14281 ccggctgggt ttccggaaga ttgatctcgc cgagtccgca gcgcgggcct gacccgagcc
    14341 agcgaatgtc attggcgatc ttcatgaggc tgaccgccag tgtgcgcaag tccccgctgg
    14401 cctcgaccaa cgaatcctgg gcggactgtc cttcgaagtg attgacggct tccttaaaag
    14461 cgcatccggt ttccttcgaa atgatggcca tgaccttgcc ggcgaatttc ggatggcaat
    14521 tgagtccggt gccgacggcg gtccctccta gagccacttc gctgagcgcc gcctgcgcgc
    14581 gcttcatccg tgcgatgccc agttcaattt gtctggcgta gccgccgaat tcctgtccca
    14641 accggaccgg cgtcgcgtcc tgcaaatggg tgcggccgat cttgacgatc ttgtcgaact
    14701 cgcgagcttt gccgttgagc gccttgtgga gttgcgtaag tgcgggaatc agttggcgtt
    14761 gaatcgtttc cgaggccgca atgtggatgg cggtcggaac cacgtcgtta ctggattggc
    14821 ccgggttcac atggtcgttc gggtgcacca gtttgctgcc cctggcgccg ccgagcagtt
    14881 ccgtggcccg gttcgaaatg acttcgttgg tattcatgtt ggtcgaggtg ccggagccgg
    14941 tttggaagat gtcgaccggg aattcggcat ccaggcgtcc ctccaccact tcggtggcgg
    15001 cgcgaacgat ggcgtccgcc ggcttcttgt cgagtagttt caacgaatgg tttaccgagg
    15061 cggcggcgcg tttgatgagt cccatggccc ggatcatcgc gcggggaata cgaagcgaac
    15121 tgatcgggaa attttcaatc gcgcgggcgg tctggacccc ataataggca ttggagggaa
    15181 ccggcagttc tcccatcgtg tcgcgctcca tgcgcgtgga ggtggtcatg gatgcggcgg
    15241 tgccggttgt gcgcgaagct ttcttcatcg tcgggtctcc tcgaatagct ggcggttgac
    15301 gtgctggagc cgcgcatctt aaacggtccc cgtgcggaag cacaagcatg gaagccccgg
    15361 ggagtggaac gtgcgctcac ccgtccgtga accaagcggc cggggccgac ggtgagacgg
    15421 tgatgcgatg gcaggcggga tggtgaaaaa gaaaaggccc gccgggggcg ggccttcctt
    15481 ctcatggcac ccgatctcgt taggagatct tgacgtcgat ctgtttcggt ttcgcctttt
    15541 ccgatttcgg gagatgcagg tgcagaacac catccgcata ctctgccctc accttgctct
    15601 cgtcgaccga atcgggaagt gtgaagctcc ggacaaaccg accgtacgac cgttcgatac
    15661 gatggtactt tttgcccttt tcttcctttt cctgtttccg ctcaccctga atggtgagga
    15721 ccgcatcttc gaccgtgacc ttcacatctt ctttcttgac ctcgggaagc tcagccttaa
    15781 tggcatactc gctctcggtt tcgctgatgt cgacggtcgg agtccagtcg gcaacggtca
    15841 tgacttcctt cccctgtccg ctagccgtgc cggttgaggg tcgcgcaatc atccggttta
    15901 accggtcgga catctcctcc aattccctga acggatccca tcgtacaatc gccatggtgg
    15961 aacctccttg tgtggatgtg ttgacgcggt tcacttggaa catcctacgt actagaaagg
    16021 aggtaatctt cacgaagccg aagtcaagcc ctgagccgga agagttaaag tcggtgacgg
    16081 ggtcttgaca ttgagacccc cggcattatt tagtagccct cttcacacag ggaggcgatg
    16141 accatgctgg aatacatgtc ttatgcgctg ctcggcggta tcatgtttgt gacatcgatt
    16201 gtcttgtggt ggtt
//
LOCUS       DAORRQ010000076        16075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_196_length_16075_cov_67.968352, whole genome shotgun sequence.
ACCESSION   DAORRQ010000076 DAORRQ010000000
VERSION     DAORRQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196_length_16075_cov_67.968352"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PK224_17595"
     CDS             complement(<1..348)
                     /locus_tag="PK224_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17615.1"
                     /translation="MKTHRALILGMLLIPLLASAGCESIKSRFARQALPDLGPPVPLT
                     IQMDVDPSLSAAKTEYIDGCGRIRPFSIGPTVEDMLIQAAHQTFRAVVLPGNHDSAGK
                     PDVTVRIRMLDPRF"
     gene            508..2241
                     /locus_tag="PK224_17600"
     CDS             508..2241
                     /locus_tag="PK224_17600"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRB17616.1"
                     /translation="MLVLGLSNMRDAAAALVSDGRIVAAAEEERFVRRKHVTALPVQA
                     MCYCLHEAGVALRDVEAIAVPWKYWQVGRRARLALTAMIRSPQLFRVKGTRSLERVSR
                     EWLELFRLREELTRRVDAGVIQPVFLDHHVCHAASSFLVSPFERSAILVVDGASEADT
                     TLMATGEGSRITVLDRTPLPHSLGQFYAAMTAFLGFRPDQDEYIVMGLASSGEPTFAP
                     ALRQNIVRLLSRGRFEVNVRLLDFHLARAGFFVEGLVRLFGAPRRSTDEITQRHRDLA
                     ASAQVVLEETLLHLGRHLRALTRAESLCLAGGVAYNCVANGRLRAELGFKEVYVPPAA
                     GDSGAALGAALWWTARREGEAGRCRLPDAYLGPQFDEAACRAALSQAGLVAESLTDAQ
                     LYERVAAELTRGRLVFWYQGRMEWGPRALGNRSLLADPRREDMRELINSKVKCRESFR
                     PFAPSVLADRAQEFFELPAPSPYMQFTVRVKASAKGLLPAVTHVDGTARVQTVTRETN
                     QRFYDLLAAFGRLTGVPVLLNTSFNVQEPIVCTPEDAVRCFQRTQVEWLVLGNLLVGR
                     SSPPAISNHAC"
     gene            2231..3772
                     /locus_tag="PK224_17605"
     CDS             2231..3772
                     /locus_tag="PK224_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17617.1"
                     /translation="MPAEPVRFSDPSPHRLIVLLLDCLLVGFILNLTLQPLVEPDLGW
                     HLRAGLDLTAQGWRLPETDPYSHTMPDWHWIEHAWLTDGLLAVIYRVMDPLSGLGLIL
                     FFSGVTALAWWVAASQARVQRTYRLVAMVASLWVALPFLGARTQLVSLLGVAILLWGW
                     GRVQAGQRAWVWGLPPLLLLWANLHGGFTAGLFLSGVLLCLSFMMRIGAGRPTVAASL
                     DEPVLSWDDLRRCVLALGIAVAVTFLNPYGWRLYQEIYESLTDRFMIETLREWQPVSF
                     QGWAGKAYGMYLAGLACLMAGWYRRVEPVRWALLLLMLGLSLRHWRNVTLFLIVSLPL
                     VAELLALAVASCVRWVPILRTHAAAALLLLTMGTAGALSALGSEHLEHVWLSGKSPDV
                     YLEQTEYPIEAVRWIRSHRQDVGTRLYNDYGYGGFLLWHFPGEKIFIDGRMPAWRIGD
                     RRIFQDYADLNREDAPRLAVLDRYAVDWGLLQRGSALALALEAHHAWERIYADAKVVI
                     VRRKG"
     gene            complement(3790..4605)
                     /locus_tag="PK224_17610"
     CDS             complement(3790..4605)
                     /locus_tag="PK224_17610"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB17618.1"
                     /translation="MTPCSTQTPSLSIIIPAYNEARRLPLCLERVIAYLDQRGRTYEV
                     LVVDDGSHDQTAQAVESVAHRCPHLRLIRLTSNMGKGAAVRRGMQAARGTYQLFADAD
                     GATPIEELARLESALLAGADLAIGSRALASQDPAFTVRARWHRSLLGTVFNNIVQRLG
                     LRDITDTQCGFKLFRGSIAQDLFSVACVDGYAFDLELLYVARQRGYRLAEVPINWVDQ
                     PGSKVRPWRDGLVMLQELLAIRKRDEQGLYEPLIRPAPSTVEPALASVEPTHF"
     gene            4799..5572
                     /locus_tag="PK224_17615"
     CDS             4799..5572
                     /locus_tag="PK224_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17619.1"
                     /translation="MRRTQCRFMWWVGALLFAGCAVATPPSRLSQYVAPVVADESPSP
                     GLPVQRPLRAALVVLADRSAQEAAPGLPEEAQARLAEALRGQVNRGFPIAIERVVNLG
                     EIPVGAPLPNWIDLAGQQGVDFVLVVILSSTEQEYPVSLFLGWTTHLQPGFRRDNWSL
                     AEAAFLDGRTGRQLLRAEGRAMATLDSPTAPGISQWYPVIYLRPQNPERRIWPPSYEG
                     APVTLRVVSMEQAAKRLAGNLQRAWVEQRELELAALPGP"
     gene            5702..7237
                     /locus_tag="PK224_17620"
     CDS             5702..7237
                     /locus_tag="PK224_17620"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRB17620.1"
                     /translation="MTYRHRRRKVLSVLAGIPMIAATLLVGGMQPGSQAQAAGVPPAF
                     AQGFSEIVKAVTPAVVNIAVTGGGEGRREGRRQLPPGGPFGGPPPGPGDEPPGMEPPG
                     GPGGPPGPPGGGPHRPEQSAGSGVILDPNGYIVTNNHVVEGATQITVTLSDRREFPAK
                     IIGTDPKTDLAIIKIEAKDLASMKWADYDELQVGDVVLAVGSPFGLSSTVTLGIISAL
                     GRGNVGIADYEDFIQTDAAINPGNSGGALVNMQGKLIGINTAIFSRTGGSEGIGFAIP
                     SSIATDIVESLTKTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSH
                     SAGMRRGDVVIAFNGKEVQSVSQLRNLVARMAVGKDADIKILREGKEQSLKVKVAERP
                     SDEVLAKREPGPAAPQTETVKPPDNVLASLRVQMLDAAMQSQLNIPAKTSGVVVSSVE
                     AGSAAESAGLQRGDVIQEVNHEVVKSLEDYQKASAKVKKDEMVVLLLSRQGNNLFVAV
                     NPK"
     gene            7318..8262
                     /gene="tatC"
                     /locus_tag="PK224_17625"
     CDS             7318..8262
                     /gene="tatC"
                     /locus_tag="PK224_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRB17621.1"
                     /translation="MADGFKFQEWLQDTVFKPLEDKKMPVMEHLVELQVRLTRAVIIT
                     AIIFVGTFFYADTLVKWIRVPLQNMFVPGSLSWIPTDLPTVPFVFLAPAEALWQNVKV
                     AGLFALVLGTPYILIEFWHFVVPGLHAQERRFVGPFVILSTLAFYLGLLFSFFFVLPF
                     ALNFLISYGVNAGFIPQLSIAQYVGFALWFLLVFGLIFEVPLVITLLAKLGWVDAPLL
                     KRYRKWAFLAAFIFAAILTPTPDPFNQCLMALPMYIFYEVGIVSAGIFNKKKAGAPEE
                     ALVPAVATAGPGLGKPGPSMPSGGGDSEYVGVPTGRRG"
     gene            8343..9299
                     /locus_tag="PK224_17630"
     CDS             8343..9299
                     /locus_tag="PK224_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRB17622.1"
                     /translation="MPRELNVISQPGSSSGGDACTYMWACAICDENETCQKDKEGHSR
                     WLVAKRMERIEYKVLVMSNKGGVGKSTCTTNLAVSLALKGWHVGICDMDIHGPNIPKM
                     VGAEGQKLKISTSGGIIPFQAYNMKIASMSFLLQNSDDPIIWRDAYKYEFINQLLGGV
                     EWQDLNFLLIDLPPGTGNESVTTIDLLGGVSGAVIITTPQEVALLDSRKSVTFCKDSE
                     VPIIGIVENMSGLECPHCHKEVNVFRKGGGEASATDMGVPFLGRIPLDPDVVTQSDAG
                     EPFALFNSDSATAQAYHDIANQVEAFCKKSGSLVKLAPRQQH"
     gene            9314..10612
                     /locus_tag="PK224_17635"
     CDS             9314..10612
                     /locus_tag="PK224_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRB17623.1"
                     /translation="MKAPDATTGLTPLHEAQRIVLDAATPLGLEKISILDTLGRVLGE
                     DIIAERHNPPWDNSAMDGFAVRAEDIQQEHTISKPVTLTVIEDVPAGKMPTKSVGRGQ
                     AIRIMTGAPIPKGADTVLKVEDTEHTPDSVRVFKPEPRGSNIRPQGEDVKKGDCIIPK
                     GTTIRSGEAGMLAILAKSFVLVYQRPRVAILSTGDELADLDERFSEEKIINSNSYGIA
                     AAVQEAGGVPILLGIARDTPAALKEKISHGLNADILVLSGGVSMGDYDFTKAVFRDLG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKLAGARGYG
                     RPLLEAVFQEKFSKRTDRRHFLRGVLWREDGVFKVRTTGDQGSGILTSMVKANCLIDI
                     PVEVERINPGDSVTVQLLSGSAWLEQAAPMASTGHRPSCC"
     gene            10633..11142
                     /gene="mobB"
                     /locus_tag="PK224_17640"
     CDS             10633..11142
                     /gene="mobB"
                     /locus_tag="PK224_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRB17624.1"
                     /translation="MSVPILSFVGRSNSGKTTLIERVIPELVRAGYKVATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVIISKSSLAMFADVSDHMNVEDVRERYLDASYDLILAE
                     GWRSEGYPKIVVVRDQIGEVPVSQDGLLAVVSNKPVETSVPLLDPDDVAGVAALIIRH
                     FPKPQRDDA"
     gene            11132..11839
                     /locus_tag="PK224_17645"
     CDS             11132..11839
                     /locus_tag="PK224_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRB17625.1"
                     /translation="MMRKSSVIVLGAIVAGAVALGGVAVPLTNHPKFCASCHTIQPAY
                     ERWLESSHKEVECVACHVRPGVSGWLQDKAWHGTRDVAIYLFGKPTAPHNLQAHVDSG
                     VCLSCHRNILRVSEIATRDLPAPVKDVGLVMSHRKHMDAFGKRGQGEGCTTCHAAVVH
                     ERPIKGYPIVIPRGHVSADSRPWKPEHPEGSVLHKRAQADCFRCHDNKTEYEGRVLSR
                     KCETCHLPEKLTEFLSF"
     gene            11860..12588
                     /locus_tag="PK224_17650"
     CDS             11860..12588
                     /locus_tag="PK224_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB17626.1"
                     /translation="MSPSAVEVRNLTKRFGGFTAVDGISFDIGRGEILGLLGPNGAGK
                     TTTFQMMLGLVTPSSGSIRMFGLDLQTNREAILQQVNFSSTYISLPYSLTVEENLGVI
                     ARLYGMSNIAGRIDDMVKKLEMSEWRNKLTRKLSSGQMTRLTLAKALLTEPKVLFLDE
                     PTASLDPDIAHKIRAILLEERQATGLSILYTSHNMREMEEMSDRIIFLQRGKIVAEGT
                     AKDIVARFGQADLEEVFLKLAREQ"
     gene            12634..13104
                     /locus_tag="PK224_17655"
     CDS             12634..13104
                     /locus_tag="PK224_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17627.1"
                     /translation="MVDAGVSFGVGLLIGGLGLMLGWGLLWLVVGAIGLARETCGWAI
                     FMSSVSSTAIAGGAMAGVLWVVDSARLTSSSLHAGLAGVPLALLVAGFCRLEDGRRIG
                     PAFLEGSRLMLHQLLGVHEEGCGHCHEKPVEEPPCHQKPCYEQSCHEKPCEGKP"
     gene            13111..13902
                     /locus_tag="PK224_17660"
     CDS             13111..13902
                     /locus_tag="PK224_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB17628.1"
                     /translation="MAYHRIAALVIRHLYLYRRSLPRVMEIIYWPFLDLVVWGFITVY
                     LATFQGQMPAVVTFLLGALILWDVLFRSQQGITISFLEEIWARNLMNLFASPLTPSEF
                     LAATMVMSLFKVTAVSMVMSVCAWIFYGYNVFIIGLWLMPFIVNLVLTGWIIGVFTTS
                     LIMRFGQEAEVLAWSMVFLFQPISCVFYPMEVLPSWLRAVASINPAAHVFEGMRGLLA
                     THAAPLSSLSLASGLNLVYLGAVILWFHHTFSVCKDRGSLVRVGE"
     gene            13938..14612
                     /locus_tag="PK224_17665"
     CDS             13938..14612
                     /locus_tag="PK224_17665"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HRB17629.1"
                     /translation="MNINSGQVRPALLPVGLEAFSFHQTPVLVIENFWSAEERTQFRQ
                     AMNRANWNQLQDLSYVRQDFPNSGNWAKAEIAQPQGQLLLSRLEMPCIQQYIESFPNI
                     TRRHLGFSYYSYGAGDCLLTHDDTDQGHPVGGKPAPRRRLAMVSYFHEEWECDWGGEL
                     MIYSATGEPTNGKPELAITHCIAPKPGSLVMFTVPRFHRVCRVDQTAGDHKRLSIAGW
                     FMTEHA"
     gene            complement(14730..15338)
                     /locus_tag="PK224_17670"
     CDS             complement(14730..15338)
                     /locus_tag="PK224_17670"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRB17630.1"
                     /translation="MEKKSKKKAPQESVATEDRKKTHVGDIVRRLRKSRHLSVRTLAD
                     KCGFSPSFISQVELRQASPSIASTERIASALGVTLGEFFRTTSPSHAAVIRADARPVV
                     ESEWSRARIEAIGPISEDSQLEPMVITLESGGASGSRPYVRRAEQLAVVLQGTVELTL
                     EENTYSLKRGDAACIPSDIHHCWRNTSRKPAQVLIVTAHRHL"
     gene            complement(15511..>16075)
                     /locus_tag="PK224_17675"
     CDS             complement(15511..>16075)
                     /locus_tag="PK224_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB17631.1"
                     /translation="IVQAGPAHEVVNHYLHAGHIQPVVEWLDPTQAPGNDVVRLRAVR
                     VRAESGIITDRFDIRKPIDLEVEFDVVKAGHIFVPVFNLYNEEDVLVFIAHDRDQAWQ
                     RTPRPTGRYTSTARIPGNWLAEGMMSVSSLMMTEDPFRMHAHAPRTIGFRVDDSGTGD
                     SARGDFHGRWPGVVRPLLEWRTKYLPA"
BASE COUNT         3018 a         4338 c         5202 g         3517 t
ORIGIN      
        1 gaatcgaggg tccagcatgc gtatccgcac cgtcacgtcc ggttttccgg cgctgtcatg
       61 attgccggga agcaccacgg cgcgaaacgt ctgatgggcg gcttgtatca gcatgtcttc
      121 tacggtcgga ccgatagaaa acggccggat ccggccgcag ccgtcgatgt attcggtctt
      181 ggcggcggac agcgacgggt caacgtccat ttgaatggtg agggggaccg gagggccgag
      241 gtccggcagg gcctggcgag caaagcggga cttgatactt tcgcagcctg cggaagccag
      301 cagaggtatt aagagcattc caagaatgag tgcgcggtgc gtcttcaagc cgaggcctcc
      361 tctacgccgg attttgaagc aggaacggta caggaaagga gggggcattg caaccgaatg
      421 ccggtgcggc ttgcgacgcg tacaggcctc gtttattcta ccgccgcttg tgtgtcggat
      481 catgaccgga agggagactg attcatcgtg ttggtgttgg gcctttccaa tatgagagat
      541 gcggcggcgg cgctcgtttc tgacggtcgc attgtggcgg cggcggaaga ggagcgtttc
      601 gttcgccgga agcacgtgac ggcgctgccg gtgcaggcga tgtgctattg cctgcatgag
      661 gccggagtcg cacttcgcga tgtcgaagcc attgccgtcc cttggaaata ttggcaggtc
      721 gggcggcgcg cgaggctcgc cctgacggcg atgatacggt ccccgcaact gtttcgagtg
      781 aaagggactc gctcattgga gcgagtcagc cgggagtggc tggaactgtt ccgattgcgg
      841 gaggaactga cgaggcgcgt ggatgcgggc gtcatccaac cggtctttct cgatcaccat
      901 gtgtgccatg ccgccagttc gttcctggtg tcgcccttcg agcggtcggc gattctcgtg
      961 gtggatggcg cgtctgaagc cgataccacc ctgatggcga caggtgaggg gagtcggatc
     1021 accgtgctgg atcgcacccc gcttccccat tcgctcgggc agttctatgc ggcgatgacg
     1081 gcctttctcg ggtttcgccc cgaccaagac gaatacattg tgatgggcct ggcgtcctcc
     1141 ggggagccga cgtttgctcc cgccttgcgt cagaacattg tgcgactact gtcccgaggt
     1201 cgatttgaag tgaatgtccg actgcttgat tttcatctcg ccagagccgg cttctttgtg
     1261 gagggattag ttcgcctgtt cggcgcgccg cggcgttcga cggatgaaat cacccaacgg
     1321 caccgcgatc tggctgccag tgcccaggtg gttctggagg aaacgttgtt gcatctcggc
     1381 cgccacttgc gcgctctcac acgggctgag tcgctttgtc tggcaggcgg ggttgcctat
     1441 aactgtgtgg ccaatgggcg gctgcgcgcc gaactcgggt ttaaggaggt ctatgtgccg
     1501 ccggccgcag gcgattcggg tgcggcgctt ggggcggcct tgtggtggac ggcgaggcgt
     1561 gaaggagagg cgggaaggtg tcgcttgccc gatgcgtatc tggggccaca attcgatgaa
     1621 gctgcttgtc gggcagcgct gtcgcaagca ggcctggtgg ccgagtccct gacggatgct
     1681 cagctctacg agcgggttgc cgccgagctg acacgtgggc gtctcgtttt ctggtatcaa
     1741 ggccggatgg agtgggggcc tcgcgcgctg gggaatcgca gcctcctggc agatccgcgg
     1801 cgggaagaca tgcgggagtt gatcaacagc aaagtaaaat gccgggagtc gtttcgaccc
     1861 ttcgctccct cggtactggc tgatcgggcc caggagttct ttgaactgcc ggcaccatcg
     1921 ccctacatgc agttcaccgt gcgggtgaag gcctcggcga aaggactcct tccggccgtc
     1981 acgcacgtcg atggaacggc ccgggttcag acggtgacgc gtgaaaccaa tcagcggttt
     2041 tatgatcttc tggcagcgtt cgggcgactt acgggtgtcc cggtgctgct gaatacgtcg
     2101 ttcaatgtgc aggagccgat cgtctgtacg cccgaagatg cggtgcgctg cttccagcgg
     2161 actcaggtgg agtggttagt cctcggcaat ctactggtgg gcagatcatc tccaccggcc
     2221 atctcgaatc atgcctgctg aaccggttcg tttttcagat ccttcccctc atcgcctgat
     2281 cgttctctta ctcgattgtc ttttggtcgg cttcattctg aatctcacgc tgcagccgct
     2341 ggtagagccg gacctgggat ggcatctgcg cgccgggctg gatttgaccg cccagggctg
     2401 gcggttgccg gagaccgatc cctactccca caccatgccg gattggcatt ggatcgagca
     2461 tgcctggttg accgatggcc tgctggcggt gatctaccgt gtgatggacc cgttgagtgg
     2521 tctgggtctg atcctgttct ttagtggcgt gacggcgctc gcctggtggg tggcagcatc
     2581 gcaggccaga gtgcaacgga cgtatcgctt ggtggccatg gtggcgagcc tttgggtggc
     2641 attacccttt ctaggcgcgc gcacgcaact ggtcagtctg ctcggcgtcg cgattctgtt
     2701 gtggggatgg ggccgcgttc aggctggaca gcgggcatgg gtctggggac tccccccgtt
     2761 gctcctgctt tgggcgaacc tgcatggcgg gtttacggcg ggactttttc tgtccggtgt
     2821 gttgttgtgc ctctccttca tgatgaggat aggcgccgga cggccgacgg tcgcggcatc
     2881 gctcgatgag ccggtcctca gttgggacga tcttcgccgc tgtgtgctgg cgctgggcat
     2941 tgcggtggcg gttaccttcc tgaatcctta tggatggcgt ctttaccagg agatttatga
     3001 gtccctgacc gatcggttca tgattgagac gttacgggaa tggcagccgg tgtctttcca
     3061 ggggtgggcg gggaaggcct acgggatgta tctggccggg ctggcgtgct tgatggccgg
     3121 atggtatcgc cgggtagaac cggttcgatg ggccctgctg ctgctcatgt tggggttgtc
     3181 gttgcggcat tggcggaacg tcaccctgtt cctgatcgtg agcctcccgc tggtggcgga
     3241 gttgctcgca cttgcggtgg cctcgtgcgt tcggtgggta ccgattctga ggacgcacgc
     3301 tgcggcggct ctgctcttgt tgaccatggg gacggccggt gccctctctg ctttggggag
     3361 cgaacatctg gagcatgttt ggctatcggg gaagtcgcct gacgtgtatc tggaacagac
     3421 ggaatatccg attgaagcgg tgcgatggat ccggagccat cggcaggatg tgggaaccag
     3481 gctctataac gattacggat acggcgggtt tctgctctgg cactttcccg gtgagaaaat
     3541 attcatcgat ggtcgtatgc cggcttggcg aatcggcgac cggcgcatct ttcaggatta
     3601 tgcagacctg aatcgcgagg acgcaccacg tttggcggtg ctggatcggt acgcagtcga
     3661 ttgggggctg ctccagcggg gcagcgcgct tgcgctggcc ttggaggcgc atcacgcctg
     3721 ggagaggatc tatgccgatg ccaaggtggt gatcgtgcga cgaaagggat gatcagttgg
     3781 acgggtctgc taaaaatggg tgggttcgac ggaagctaag gcgggttcga cggtgctcgg
     3841 tgcgggacga ataaggggct cgtataaccc ttgctcgtca cgtttccgga tggccaggag
     3901 ttcttgcagc atgacgagtc cgtcgcgcca ggggcggact ttcgagcccg gctggtcgac
     3961 ccaattgatc gggacttccg caagccggta gccgcgctgt cgcgcgacgt agagcagctc
     4021 caggtcgaac gcatagccgt cgacacaggc gactgaaaac aaatcttgag cgatcgatcc
     4081 acgaaacagc ttgaatccgc attgagtgtc ggtgatatcg cgaagaccca ggcgctgcac
     4141 gatgttgttg aagacggttc ccaacaggct tcgatgccac cgtgcccgga cggtgaaggc
     4201 gggatcttgg gacgccaacg cccgtgatcc gatggcgaga tccgctcctg ccagaagcgc
     4261 cgattccagc cgtgccagtt cttcgatcgg tgtggcccca tccgcgtccg caaacaattg
     4321 atacgtgccc cgcgcggcct gcatgccacg gcggacggcc gcgcccttgc ccatattgct
     4381 ggtcaatcga atgagtcgca ggtgcgggca gcgatgggcc accgactcga cggcctgcgc
     4441 ggtctggtcg tggctgccgt catcgacgac gaggacttca taggtccggc cgcgctgatc
     4501 caggtatgca atgactcgtt ccaggcagag tggaagacgg cgcgcctcgt tataggccgg
     4561 gatgatgatc gaaagagaag gcgtctgagt cgagcacggt gtcacgttgg gacagcctcg
     4621 tgtcggagtg tgacgggacc gatccgtctg ttgaaggcga atggtggccc acggtatctg
     4681 accgaggggc gaaatgcaag ccatgggtgc cgggtcttga cagagccatc gaccgggggt
     4741 gatcgtaatc tcacagactg taggtgccca ggcgtcgctc tctggccagg aggttgcgat
     4801 gaggcggaca cagtgcaggt ttatgtggtg ggtcggggcg ctgttgtttg ccggttgcgc
     4861 cgtggcgact ccacccagcc ggttaagcca gtatgtcgcc cctgtcgtgg cggacgaatc
     4921 accaagcccc gggcttccgg tgcagcgccc gcttcgagcc gcgctggtcg tgctggccga
     4981 tcggtcggct caggaggctg ccccgggttt gcctgaagaa gctcaggcaa ggctggctga
     5041 ggcgctcagg gggcaggtca atcggggctt ccccatcgcc attgaacggg tggtgaatct
     5101 cggtgagatt ccggtcggag cgcctcttcc gaattggatc gaccttgccg ggcagcaggg
     5161 tgtggatttt gtcctggtcg tgatcctttc gagtacggag caggagtatc cggtatcgct
     5221 gttcctcggc tggacgaccc acctgcagcc cggattccgt cgcgacaatt ggtccctcgc
     5281 ggaagccgcc ttcctcgatg gacgaacagg tcggcagctt ctccgtgcgg aggggcgagc
     5341 catggcgacg ttggacagcc ccacggcgcc agggatttcg cagtggtatc cggtgattta
     5401 tctgcgaccg cagaatccgg agcgacgcat ctggccgcca tcctatgaag gcgcccctgt
     5461 gacgctccgt gtggtgtcga tggagcaggc ggcgaagcga ttggccggga atctgcagcg
     5521 agcctgggtc gaacagcggg agctggagtt ggccgcgctg cccggaccat gactcctgtc
     5581 caccggagca acgggccctg ctttttcctc tttgaaggcg attctgtgat tagtgtagaa
     5641 tgattcgttc acgatggcgc ggaggatggt ccctcatttt caggcttcca gaggagtaca
     5701 catgacgtat cgacatcggc gcaggaaagt gctgtccgta ctggcaggga tccctatgat
     5761 tgctgccacg cttttagtcg gcggaatgca gcccgggtct caggctcagg cggcgggtgt
     5821 ccccccggcg tttgcgcagg gattctcaga aatcgtcaag gcggtcacgc ctgcggtcgt
     5881 caacattgcc gtcaccggtg gaggggaagg ccgtcgtgaa ggccgtcgtc agcttcctcc
     5941 aggcggacca ttcggtggcc cgcctcctgg acccggtgat gagccgcccg gcatggaacc
     6001 gccaggtggt cccggtggac ctcccggccc tccgggtggt ggcccccatc gccccgagca
     6061 gagtgccgga tccggtgtga tcctcgatcc gaacgggtat atcgtcacga ataaccacgt
     6121 ggtcgaaggc gcgacccaaa tcaccgtgac cctgtcggac cgtcgtgaat ttccagccaa
     6181 gatcatcgga accgatccga agacggattt ggccatcatc aagattgaag ccaaagatct
     6241 cgcgtccatg aagtgggccg attatgatga attgcaggtc ggtgacgtgg tgctagcggt
     6301 gggcagcccg ttcggactga gctcaaccgt gaccttgggc attatcagcg ctctgggacg
     6361 cgggaacgtg ggcattgccg attacgaaga ctttattcaa accgatgcgg cgatcaaccc
     6421 cgggaattcc ggcggcgctt tggtcaatat gcagggcaag ttgatcggca tcaacacagc
     6481 gatcttctcc cgcaccggcg ggtccgaggg catcggcttt gccattccga gcagcatcgc
     6541 gaccgatatc gttgagagtt tgacgaagac cggcaaagtg gtgcgcggat ggatgggtgt
     6601 ggccattcag gaaattaccc cggcgctggc gaaatccttc aaattgccgg agcagcgcaa
     6661 aggcgtgctt atcagcgacg tgaacgagaa tgggccctct cattcggccg gcatgcggcg
     6721 tggtgacgtg gtcattgcct tcaatggtaa agaggtacag agcgtcagcc aattgcggaa
     6781 cctcgtggcc cgtatggctg tcggcaagga tgccgacatc aagattttgc gcgaagggaa
     6841 agagcagagc ttgaaggtga aggtggctga gcggccgtcg gacgaggtcc tcgctaagag
     6901 ggagccgggt cctgccgccc ctcagacaga gaccgtgaag ccgccggaca atgtcctggc
     6961 gtccttgcgc gttcagatgc ttgatgccgc gatgcagagc cagctgaata ttcccgcaaa
     7021 aaccagcggc gtggtggtca gttcggttga ggccggcagc gccgccgagt ctgcagggtt
     7081 gcagcgtggc gatgtgattc aagaggtcaa ccacgaagtg gtcaagagcc ttgaggatta
     7141 ccaaaaggca tcggccaagg tgaagaaaga cgaaatggtt gtgctcttgc tgagtcggca
     7201 ggggaacaat ctgttcgtag ccgtcaaccc gaaatagaca gcaaccagcg gacagctttc
     7261 agctatcagg ccaattccgg ctgatggctg aaggcctcca gctgaaagcg attgtgaatg
     7321 gctgacgggt tcaagtttca agagtggctg caagatacgg tctttaagcc gcttgaagat
     7381 aaaaaaatgc cggtcatgga gcacctggtg gagctccagg tccggctgac ccgtgcggtc
     7441 attatcacgg cgatcatttt tgtcggcaca ttcttctacg ccgacaccct ggtgaagtgg
     7501 atcagggttc cgctccagaa tatgttcgtt cccggatctc tttcctggat ccccaccgat
     7561 cttcccaccg tccccttcgt gtttctcgcg ccggctgaag cgctgtggca gaacgtcaag
     7621 gtggccggcc tctttgccct ggtattgggg actccgtata ttctcattga gttctggcat
     7681 tttgtggtgc cgggtctgca cgcgcaggag cggcgattcg tcggtccgtt tgtgatcctg
     7741 agtacgctgg cgttctacct gggcctgttg ttctcgtttt tcttcgtgct cccctttgct
     7801 ctgaactttt tgatttcgta tggcgtgaat gcgggcttca ttccgcaact gtcgattgcg
     7861 caatatgtcg gattcgcgct gtggttcctt ctggtcttcg gtctcatttt tgaagtgccg
     7921 ttggtgatta ccctgctggc caaacttggc tgggtggatg ccccactgct gaaacgatat
     7981 cgcaagtggg ccttcctcgc ggcgtttatc ttcgccgcga ttctgacgcc gacgccggac
     8041 cccttcaatc agtgcctgat ggcgctgccg atgtatattt tttatgaggt cgggatcgtg
     8101 agtgccggaa tcttcaacaa gaagaaggcg ggcgctcccg aagaggcgct tgtgccggcg
     8161 gtggccacgg cgggtcccgg tctcgggaag ccgggaccgt ccatgccgtc cggcggcggt
     8221 gatagtgaat atgtcggtgt gccgactggt cgtcgagggt aacgcagggc aggaatcagc
     8281 aacgtgacga acgcacatgt ttgtcccacg catcatgata tacgctttat gaggagtcta
     8341 ggatgccacg tgagttaaac gtcatttcac agcccggttc cagcagcggc ggagatgcct
     8401 gcacctatat gtgggcctgc gccatttgcg acgagaatga aacctgtcag aaagacaagg
     8461 aaggccacag ccggtggctc gtcgccaaac gcatggaacg gatcgaatat aaggtcctgg
     8521 tcatgagcaa caagggtgga gtggggaaaa gcacctgcac caccaacctg gcggtcagcc
     8581 tcgcgctcaa aggctggcat gtcgggattt gcgacatgga cattcatggt ccgaacattc
     8641 ccaagatggt cggggcggaa ggacagaagc tgaagatcag cacgtccggg ggaatcattc
     8701 cgtttcaagc ctacaacatg aagatcgcgt cgatgtcgtt cctgttgcag aactccgatg
     8761 atccgatcat ctggcgcgac gcgtataagt atgagttcat caatcagttg ctgggagggg
     8821 tggaatggca ggatctcaat ttcctgttga tcgatctgcc cccaggcacc ggcaacgaat
     8881 cggtgacgac tatcgatctc ttgggcgggg tcagcggagc ggtgatcatt acgacgccgc
     8941 aagaagtcgc gctgctcgat tcccgcaagt ccgtgacctt ctgtaaagac agcgaagtgc
     9001 cgattatcgg catcgtcgag aacatgagcg ggctggaatg tcctcattgc cacaaggaag
     9061 tgaatgtctt ccgtaagggc ggcggtgaag cgtcggctac tgatatgggc gtcccgttcc
     9121 tgggccggat tccgctcgat cccgatgtcg tgacacaaag tgatgccggt gagccattcg
     9181 cgctctttaa ttccgattcg gcgaccgcgc aggcctatca cgacatcgca aatcaggtcg
     9241 aagcgttctg caagaagagc ggatcgctgg tcaagctggc gccgcgtcag caacattgat
     9301 gggacggtga cgcgtgaaag ctcccgatgc gacaaccgga ttgactccgc tccacgaagc
     9361 tcagcgcatt gtgctggacg ccgcgactcc gctggggctt gaaaagatct cgattctgga
     9421 taccctgggg cgcgtgctcg gcgaagatat catcgctgag cgccataatc cgccgtggga
     9481 caattccgcg atggacggat tcgcggtgcg ggcggaggac attcagcagg agcacacgat
     9541 ctccaagccg gtcacgttga cggtgatcga ggatgtgccg gccggaaaga tgcccaccaa
     9601 atccgtgggg cggggccaag ccatccggat catgaccggc gcgccgattc cgaagggcgc
     9661 ggataccgta ctcaaggtcg aggacacgga acatacgccc gattcggtgc gcgtattcaa
     9721 gccggaaccc cgtggctcaa atatcaggcc gcagggtgag gatgtgaaaa agggcgattg
     9781 catcattcca aaaggaacga caatccgttc cggcgaagcg gggatgctgg ccattctggc
     9841 caaatctttc gtgttggtct atcagcggcc gcgagtcgcg attttatcga ccggcgatga
     9901 gttggccgat ctcgatgagc gcttcagcga agagaaaatc atcaactcga acagctacgg
     9961 gattgccgct gcggtgcagg aagccggcgg cgtgccgatc ctgttgggga ttgcgcggga
    10021 cacgccggcc gcgttgaaag aaaagatttc gcacggattg aatgccgata ttctcgtgct
    10081 ctcaggcggc gtgtcgatgg gggactacga cttcacgaaa gcggtcttcc gggatttagg
    10141 cgccgagatg aatttctgga agttggcgat ccgcccaggt cagccgcttg ccttcggaaa
    10201 gattcagggc aaactggcat tcgggttacc ggggaatccc gtttcctcga tggtgacctt
    10261 cgagcaattg gttcggcctg ccatgctgaa actggccggg gcgcgaggat atgggcgtcc
    10321 gctgcttgag gcggtgtttc aggagaagtt ttcgaagcga accgatcgcc gtcattttct
    10381 ccgcggagtg ttgtggcggg aagacggggt cttcaaagtc cggacgaccg gcgatcaggg
    10441 gtcaggcatt ctcacctcca tggtcaaagc caattgtctg atcgacattc ccgtcgaggt
    10501 ggaacggatc aatcccggcg actcagtcac cgtacagctt ctcagtggat cggcatggct
    10561 ggagcaagcg gccccgatgg catcgaccgg tcatcgtcct tcctgttgtt gagccgcgaa
    10621 aggaacatgt tcatgtccgt gccgatttta tcttttgtcg gacgatccaa tagcggtaag
    10681 acgacgctga tcgagcgtgt catcccggaa ctggtgcggg cggggtataa ggtggcgacc
    10741 gtcaaacatg ccgggcatgg attcgatctg gataccgaag gaaaagacag ctggcggcac
    10801 aaacaagccg gggcgagcag tgtggtcatc atttccaaga gcagcctggc catgttcgcc
    10861 gatgtgtcgg accatatgaa cgtcgaggat gttcgtgaac ggtatctgga tgcgtcctac
    10921 gacttgatcc tcgctgaagg ctggcggagc gaagggtatc ccaagatcgt agtggtccgc
    10981 gatcagatcg gcgaggtgcc ggtttcgcag gatgggctac tggctgtggt gtccaataag
    11041 ccggtcgaga cctcggtccc gctcctcgac cccgacgatg tcgccggcgt cgcggcattg
    11101 atcatccgcc actttcccaa accccaacgg gatgatgcgt aaatcctccg tcattgtgct
    11161 cggagcgatc gtggccggcg cagtggcttt agggggagtt gcggttccgc tgaccaacca
    11221 cccgaaattt tgtgccagct gtcacaccat tcagccggcc tatgagcgct ggctggaatc
    11281 atcccataaa gaggtggagt gtgtggcgtg ccatgtcagg cccggcgtct caggttggtt
    11341 gcaggacaag gcgtggcatg gaaccaggga tgtggccatc tatctcttcg gcaaaccgac
    11401 cgcgccccac aatctgcaag cgcatgtcga ttcgggcgtg tgtctgagtt gccatcggaa
    11461 catcctccgg gtgtcggaga ttgcgactcg ggatctgccc gcacctgtga aggatgtggg
    11521 attggtcatg agccatcgca agcatatgga cgcattcggg aagcgagggc agggcgaggg
    11581 ctgcacgacc tgtcacgcag cagtcgtgca cgagaggccg atcaagggct accctatcgt
    11641 gattcctcgc gggcacgtgt cggccgatag ccggccctgg aagcctgaac atccggaagg
    11701 ttccgtcctg cacaagcggg cgcaggcgga ttgcttccgc tgccacgata ataaaaccga
    11761 gtatgaaggt agagtcctga gccggaagtg cgagacgtgc catctgcctg agaaactcac
    11821 agaattcttg tcgttttaat ccacgggaat actcgctcca tgtcgccctc ggccgtcgaa
    11881 gtgcgcaatc tgaccaaacg cttcggcggg ttcacggcag tggacgggat ttcgttcgac
    11941 atcggccggg gtgagattct cgggctgttg ggcccgaatg gagcaggaaa aacgacgacg
    12001 tttcagatga tgctgggact ggtcacaccc agttccggct cgatccggat gttcggactc
    12061 gacttgcaga ccaatcggga ggcgattctt cagcaggtga atttttcctc cacgtacatc
    12121 tcgttgccct attccctgac ggtggaggaa aatctcggtg tcatcgctcg gttatacggg
    12181 atgtccaaca tcgccggccg aatcgatgac atggtgaaga agctggagat gagtgagtgg
    12241 cgaaacaagc tcacccgcaa actttcgtcg gggcagatga ccaggctcac attggccaaa
    12301 gcgttgctca cggagcccaa ggtgctgttt ctcgatgagc ccacggcaag cctcgatccg
    12361 gacatcgcgc acaaaatccg cgcgatcctc ctcgaagaac ggcaggccac cgggctcagc
    12421 attctctaca cctcccacaa catgcgggag atggaggaaa tgtcggatcg tattatcttc
    12481 ctgcaacggg gcaaaatcgt tgcggagggc acggcgaagg acattgtcgc cagattcgga
    12541 caggccgatc tggaagaggt gtttctcaag ttagctcgcg agcagtaacg gcagatcggg
    12601 atcgctgctt cgtggaaggg agtacgggca gtcatggttg acgcaggagt gtccttcgga
    12661 gtgggtctgc tcatcggcgg gttgggcctg atgctgggat ggggattgtt gtggctcgtc
    12721 gtcggcgcca ttggtctggc acgagagacg tgcgggtggg cgatcttcat gagcagcgtc
    12781 agcagcacgg ccattgcggg gggggcgatg gcgggcgtgc tctgggtggt ggattcggcg
    12841 aggttaacta gttcttcgct gcatgccggt ctggccgggg ttccgctggc gctgcttgtc
    12901 gcagggttct gtcgtttgga ggatggtcgg cggatcggtc cggcgttcct ggaaggcagc
    12961 cgcctcatgt tgcatcaact gcttggtgtg catgaggagg gatgcgggca ttgccacgag
    13021 aaacctgttg aggagccgcc ctgtcaccag aaaccctgtt acgagcagtc ctgccacgag
    13081 aagccttgtg aagggaagcc ctagctgtcg atggcctatc atcgtattgc agccctggtg
    13141 attcgtcacc tgtatctcta ccgtcgcagt ctcccgcggg tgatggagat catctactgg
    13201 ccgtttctgg acctggtggt ctggggattc atcaccgtct atctggcgac ttttcaaggg
    13261 cagatgccgg cggtggtcac gttcctcctc ggggcgttga ttctctggga cgtgttattt
    13321 cgctcgcagc agggtattac catttcgttt ctcgaagaaa tctgggcgcg taatctgatg
    13381 aacctcttcg ccagcccgct cacgccgagc gaatttctgg cggccaccat ggtgatgagc
    13441 ctgtttaaag tgaccgcggt gtcgatggtg atgtcggtct gcgcctggat cttttacggc
    13501 tacaacgtct ttatcatcgg gctctggctg atgccgttca tcgtgaatct ggtgctgaca
    13561 ggctggatca tcggtgtgtt cacgacctcg ctgatcatgc ggtttgggca ggaggcggaa
    13621 gtgttggcct ggagcatggt ctttctgttt cagccgatct cctgcgtatt ttatcccatg
    13681 gaggtcctgc cgtcctggct gagagctgtg gcttccatca atccggccgc ccatgtgttt
    13741 gagggcatgc gcggcctgct ggccacccat gcggctcccc tgagcagcct gagcctggcc
    13801 agcgggttga atcttgtgta tcttggagcg gtcatcctat ggtttcatca caccttcagt
    13861 gtgtgcaagg accggggatc gttggtgcgg gtcggtgagt aggtgatcga ccgaacaggg
    13921 aatgtgggag tagtgggatg aatatcaact cagggcaagt caggccggca ctgctgccgg
    13981 tcggtctcga agccttttcg tttcatcaga cgccggtcct cgtcatcgag aatttttggt
    14041 ccgccgagga gcggactcag tttcggcagg cgatgaaccg tgcgaattgg aatcagttgc
    14101 aagatttgtc atacgtgcgg caggacttcc ccaattccgg gaactgggcg aaggccgaga
    14161 tcgcccaacc gcaaggccaa ctcctgctga gccggttgga gatgccctgc attcagcagt
    14221 atatcgagtc atttccgaac atcaccaggc ggcatttggg cttcagctat tattcctatg
    14281 gtgccggtga ctgccttctg acccacgatg atacggatca aggtcatccg gtcggtggga
    14341 agcccgctcc ccgtcgccgt ctcgccatgg tgagttattt ccatgaagaa tgggaatgtg
    14401 attggggcgg agagttgatg atctactcag cgaccggcga accaacgaac ggaaagccgg
    14461 aactggccat tacgcactgc atcgcaccca agccgggatc attggtcatg tttaccgtgc
    14521 cgcggttcca tcgggtgtgc cgggtggatc aaacggcggg agaccacaag cgcctctcga
    14581 ttgccggctg gttcatgacc gaacatgcct gagcgtgtat cctattgcag aacaatcgat
    14641 tgcagcgtga gcggagattg ccgggtggtg tgtttgcaag tcagcggaac acggcaaaat
    14701 atttttcaga agttgagaag ggcgagtggt tacaagtgcc gatgggcggt cacgatgagg
    14761 acttgggccg gttttcggct cgtgttgcgc caacaatgat ggatatcaga aggaatgcag
    14821 gcggcatccc ctcgtttgag gctgtacgtg ttttcttcca atgtcagttc gacggtaccc
    14881 tgaagcacga ctgccaattg ttccgcgcgc cgtacatacg gacgtgatcc gctggcccca
    14941 ccagactcca gtgtaatgac catgggctcc agctgactgt cttcgctgat tggtccgatc
    15001 gcttcgattc tggcccgaga ccattcgctt tccactaccg gccgggcatc ggcgcggatg
    15061 acagccgcat gtgatggact ggtcgttctg aaaaattctc cgagggttac gccgagcgcc
    15121 gatgcgatcc gctcggtgga ggcaatagac ggtgaggcct gccggagttc gacctgggag
    15181 ataaaactag gggagaagcc gcatttgtcc gctagggttc ggacagagag gtgtcggctt
    15241 ttgcggagac ggcggacgat gtcgccgaca tgcgttttct tccggtcctc ggttgctacg
    15301 ctctcctgcg gggccttctt cttggatttt ttctccacgt cgtcctatcc tttgggtcct
    15361 acccaggatt cgcgcggttg gtgtagcacg tttatgggcc tgtcgcaagc cggccgaagg
    15421 ggcggaatct tctcagacat atctgcggtt gtcttccaat agagagagtg ttgtcaccat
    15481 gggaccaccc tgctgtgtct ctgcagcctc ttatgccggt agatacttgg ttctccactc
    15541 caacaacggc cgaacgactc ccggccaccg accgtgaaag tctcctcgcg ccgaatctcc
    15601 ggtcccgctg tcatccacac gaaatccaat tgttcggggc gcatgggcgt gcatgcgaaa
    15661 aggatcttcg gtcatcatca gggatgagac cgacatcatt ccttcggcaa gccagtttcc
    15721 tggaattctg gccgtggaag tatatcgacc ggtcgggcga ggggttcgct gccaggcctg
    15781 gtcccggtca tgggcgataa aaacaaggac atcttcctca ttgtagagat taaagaccgg
    15841 cacaaagata tgcccggctt tcacgacatc aaattccact tcgagatcaa tcggttttcg
    15901 aatgtcgaag cggtccgtga tgatgccgga ctcagcccgg acgcgtacgg cgcgcaagcg
    15961 gacgacgtca ttgcccggag cctgggttgg atccagccat tccacgaccg gctggatgtg
    16021 tccggcatgg aggtagtgat tcaccacttc atgcgccggc cctgcctgca cgatc
//
LOCUS       DAORRQ010000077        15508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_204_length_15508_cov_10.660713, whole genome shotgun sequence.
ACCESSION   DAORRQ010000077 DAORRQ010000000
VERSION     DAORRQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204_length_15508_cov_10.660713"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>417
                     /locus_tag="PK224_17680"
     CDS             <1..>417
                     /locus_tag="PK224_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17632.1"
                     /translation="PLDRYEAYQESRRKQEEATPEQNPEDDKGQPLPYRLGTSVSNYW
                     IPLLPVMDGTSLKLKRGTIPDFNAGERPRLIEPMGLILEPGRELLLQEEEVPREGARV
                     TRTYQYARWIDGSTHLWIGRRKEPGRGEGSSGLQFDV"
     assembly_gap    418..434
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            484..1506
                     /locus_tag="PK224_17685"
     CDS             484..1506
                     /locus_tag="PK224_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17633.1"
                     /translation="MRIKIDRWAKAACANSDRMTSTLPSQNFFLYLNIDVFPAAWAAY
                     RSTLITPANYGTPSFRPISYWPYNLLKTKRTLRLQKEIELELIRKTFYSAQVSRLHGI
                     YLWGDEQSAKRGERWRETEGNHFHADYLVEVGFTYNRLTKVDTNWIDEYLLHDSTPLD
                     RNCLDWMHSYRRGEPWPTAEPLWEYIAEGRGLVYGTALRKQAYEKVKTLAPDSLGQLE
                     LGRIAVEMGSDLYQLSPFIRQLSPTSFTVDFHLDSHDENDQFMEKAGDHIKRIMEKDR
                     THINHEALDLLRGDTRRPDLRSMGVAFDCSEFSIEAKTLIESLVSAHDGSIWSGLTEP
                     PSAKTP"
     gene            1969..2112
                     /locus_tag="PK224_17690"
     CDS             1969..2112
                     /locus_tag="PK224_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17634.1"
                     /translation="MTRLHLKRLYARWIDGTTHLWIGRPKNPGQGEGSSGLQFDSVES
                     PGG"
     gene            complement(2217..2702)
                     /locus_tag="PK224_17695"
     CDS             complement(2217..2702)
                     /locus_tag="PK224_17695"
                     /inference="COORDINATES: protein motif:HMM:NF018748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRB17635.1"
                     /translation="MIVNALEQPRRPFPPFDAESATQKVRMAEDAWNTRNPETVSLAY
                     SADSRWRNRSEFLTGREAIVNFLRRKWENERDYRLIKELWAFHGARIAVRFAYEWHDE
                     TGSWFRSYGNENWEFDENGLMRRRIASINDLPITASERKYHWALGRRPDGHPSLSDLS
                     L"
     gene            complement(2783..3142)
                     /locus_tag="PK224_17700"
     CDS             complement(2783..3142)
                     /locus_tag="PK224_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17636.1"
                     /translation="MSKSVKDTLEEMLGILKEGQMTEGQKIYFADSVVTQEGDQASVV
                     GKQAAIERLDKFRETIGVAAFVSYTIGAVAISGSTSFYDSVLTVKLKNGQTISLEQVV
                     KTDWQDGKIVKERYYHS"
     gene            complement(3157..3312)
                     /locus_tag="PK224_17705"
     CDS             complement(3157..3312)
                     /locus_tag="PK224_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17637.1"
                     /translation="MNAAAIDNAAQSTMDSTGLAQGTAEGGGILIVQPSPVWEFLVVI
                     QMKRRKA"
     gene            complement(3435..4103)
                     /locus_tag="PK224_17710"
     CDS             complement(3435..4103)
                     /locus_tag="PK224_17710"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HRB17638.1"
                     /translation="MQVEQIWRYPVKSMAGERLEHARIGALGIEGDRVVHVEDAQGHV
                     ITARSHYRLLGHRATLNGSGEPVVDGRLWSDPNVRMDVEDIVGPGAMLVRDDSSDRFD
                     VLPLLVATDGAVAAFGHDGRRLRPNLVIGGVEGLAERSWPGQCLHIGEVIIGIQDLRG
                     RCIMTTFDPDSLKQDRQVLTDIVRRFGGTLALNCFVIRGGDVRVGDTVELARHRQCEA
                     AHAL"
     gene            complement(4160..4750)
                     /locus_tag="PK224_17715"
     CDS             complement(4160..4750)
                     /locus_tag="PK224_17715"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRB17639.1"
                     /translation="MAMKYLETMMTDSVLRAQHHCYGHAANITGAPERDLLGEGEAEF
                     LAARDSFYLSTVSETGWPYVQHRGGPKGFLRLLNPSTLVFADYQGNRQLLSTGNLSVD
                     DRVSLFLMDYPNRARLKILGHARVEDARLHPDLAVQHANPGMKARVERIVFINVVSFD
                     WNCPKYITPRYSLEEIEELMGTLKARIAELETQLSR"
     gene            complement(4823..5197)
                     /locus_tag="PK224_17720"
     CDS             complement(4823..5197)
                     /locus_tag="PK224_17720"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRB17640.1"
                     /translation="MKTAIIVMSDPKNGSEEALGRVFNALALAAECKQEGDEVAVVFN
                     GTGTRWPAELTKLSHPANGLYNAVRDVVQGASCGCADVFGAKESVEACGVPLKKDHAL
                     AGTSGLLSLRRYVNEGWNTVVF"
     gene            complement(5455..7029)
                     /locus_tag="PK224_17725"
     CDS             complement(5455..7029)
                     /locus_tag="PK224_17725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRB17641.1"
                     /translation="MDGSPSSPSSHSHSALDRIAMQMASSLDLHIVLTTITQGLVDDL
                     DAAFARIWLRGPGDLCTGCYKASDCRDRTQCLHLRASAGLYSNLDGEYRRIPLGSLKI
                     GKIAQGFGPMQTNDVLGDDRLPNKQWMKDHGLHSFAGYPLVFRGDVLGVIAMFGRRPL
                     ADEEWDRLATFAHQAATAIRNAQLFTEVEQLTNRLQAENMYLREEIKLDHHFGEIIGE
                     SQAMKTVLRKVEQVAPTDSTVLLLGETGTGKELIARAVHDLSPRSARPLVRVNCGAIP
                     ANLVESELFGHEKGSFTGALAKRIGRFELAEGGTIFLDEVGELPLDAQVKLLRVLQER
                     EFERVGSGRSTKVNIRVIAATNRDLPAAVKAGSFRSDLLYRLNVFPIELPPLRNRSSD
                     IPLFVRSFAGRLSKKFGKRLDQISQQTMDRLIHYAWPGNVRELENVIERAAILSPGPV
                     LQVEEVLLQSDRVATSDTSYTLEEVERTHITRVLQEVDWVVEGKQGASTRLALHPNTL
                     RSRMQKLGIKKPRPSS"
     gene            complement(7189..7371)
                     /locus_tag="PK224_17730"
                     /pseudo
     CDS             complement(7189..7371)
                     /locus_tag="PK224_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7903..8394)
                     /locus_tag="PK224_17735"
     CDS             complement(7903..8394)
                     /locus_tag="PK224_17735"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRB17642.1"
                     /translation="MKRIKKMQAPRAKGKSSVSPCISKLPRHRLVELLWSFAPAYQRW
                     SESLLVEKGLSPQRLRILGSIHERGPRIMSELKKELGVTATNVTALVDSLEKDGLVVR
                     TRHPTDRRATVIELSDKVMTELSPRCTEYKEQVAELFSDLTDQECKTFVKTLEKLWSR
                     LQG"
     gene            8584..9750
                     /locus_tag="PK224_17740"
     CDS             8584..9750
                     /locus_tag="PK224_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17643.1"
                     /translation="MITLRYTPWLLAFLLTVTGCNSEQASSGGQAPPPEVVVAQVLSK
                     SVQQWDEYTGRITAIDTVELRPRASGYVQRVAYKEGQDVKQGDLMFQIDPRPYRAALD
                     NALAQLARARAAKRLETIRNTRAQSLIHDDAISHEELDLRRAAQEQSAADVQAAEAAV
                     ATAKLNLSFTEVRAPVSGRASRALLTVGNLATADETLLTTVVSQNPMYVYFDADENSY
                     LRYKEQERKSERTAQDNAVHVGLANETGYPHTGRVDFLDTQVNPTIGTVRARAVLPNA
                     DRIFTPGLFARVQFVSGQKAQVLLIDDKAILTDQDRKYVYAVDRDGKAQRKDIVSGGM
                     VDGLRVVQSGLAPDDRIVVVGLQKIFYPGMPVIPAEVPMDKSSTPAAPAAMAEK"
     gene            9763..12960
                     /locus_tag="PK224_17745"
     CDS             9763..12960
                     /locus_tag="PK224_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRB17644.1"
                     /translation="MDFSKFFIDRPIFAAVLSIVIFAAGLIAIPILPISEYPEVVPPA
                     VIVRAVYPGANPKVIAETVSTPLEEQINGVENMMYMKSVAGSDGVLQMTVTFRPGTDP
                     DAAQVQVQNRVSQALSRLPSDVVSLGVTTQKQSPTFLVMVQLLSPDGKYDALYLRNYA
                     NIKVKDELARLPGVGQVQIFGSGDYAMRIWLNPDKIASRGMTAGDVVAAVREQNIQVS
                     AGQLGAEPIAKGTDFLISINAQGRLRTAQEFGNVVLKIGAGGEVVRLSDVARVELGAN
                     DYTLRGQLDNQDAPPIGIFQAPGANALTVRDAVIAKMEELKTRFPPGLTYRSDYDTTV
                     FVRDSIQAVVQTLMEAILLVVLVVILFLQTWRASIIPLIAVPVSVVGTFAALYLFGFS
                     INTLTLFGLVLAIGIVVDDAIVVVENVERNIEEGRTPLQAAHQAMQEVSGPIVAIALV
                     LCAVFVPMAFLSGVTGQFYKQFAVTIAISTVISAINSLTLSPALAAKLLKGHGTPKDA
                     LSRWIERLFGWVFRPFNRFFKASSDRYQGTVSRTLKHRGAVFAVYALLLLATGLMFQA
                     VPRGFIPTQDKLFLMAGVKLPEGASLERTDAVLRQVVAISKTVDGIAHVVAMTGLNPL
                     QFTNTPNYGIAFLILKPFDERHRSAKEISEEMNQKISGIKEGITFVLMPPPILGLGNG
                     AGYALFVEDRAGLGFGALQNAVSGLQGAVMQTPGMGYPISGYQANVPQLDAEVDRVKT
                     KTQGIALTELFNTLQVYLGSAYVNDFNLFGRTWRVYAQADGDFRGKVEDIANLKTRND
                     RGEMVPIGSMVKFSQTYGPDPVLRYNGYPAADFMGEADPTKLSSAQAMDNIRDIAAKV
                     LPNGMTIEWTDLSFQEASQGKAALLVFPVAILLAFLVLAALYESWTLPLAVILIVPMC
                     MLCALAGVKLTGGDNNTFVQVGLVVLMGLACKNAILIVEFARELELQGKEIVEAALEA
                     CRLRLRPIVMTSVAFIAGTIPLVLSHGAGAEVRSATGVTVFAGMIGVTLFGLFLTPVF
                     YVGLRMLSGRKLVRHDESMEHGMVEGAYRQVDSPR"
     gene            complement(13050..13511)
                     /locus_tag="PK224_17750"
     CDS             complement(13050..13511)
                     /locus_tag="PK224_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB17645.1"
                     /translation="MMNMIDGIRQLTVPMVALCMLCLMPAYALAVTGDEATHEADHAL
                     DADIIELPEVHVHGLPLNKDQQVGPVPKSTPWPAIPASLDGKPLDDWMKARLLVSKEA
                     QVTVVVLEPAKHRELTVAGIIALNKWTFAPQLKGDDPIDGELTVRIHFRSQ"
     gene            complement(13830..>15508)
                     /locus_tag="PK224_17755"
     CDS             complement(13830..>15508)
                     /locus_tag="PK224_17755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB17646.1"
                     /translation="MGGSNKTINPSFEYGGTIGEKFRFYILNSHTATNRGIEPPTLGR
                     SVYHGQSERNQTFIRGDYQHDNRNNFTWLFLNSVAKYQIPTSPGGELDPSGQMLPLLQ
                     GSRPGFSPVASQAIDEFQKENNQYGHMVWRHDVNANNFFSLSGYMRHTRATFKTDPLN
                     LLAYTADPAEPFSAADQDRNAYSTGVRFDYTYTHSKQHLIKAGFQLDRTQSVNKTRLF
                     TFADDGGGNPTGDLLSLNADNRQIGYRQEFWIQDQWSPNDQWTFNLGLRGDIVQYQRN
                     EGQVSPRIGVSYKYNPSNVFHVFYGRMFTPPNLEAISFAKLNTLGTRAQPEDVTNNTV
                     RAERAHYFEVGSYHALNRYATLQFTGWYKLSNFLSDAGQFGTTPLLNYFAFERGWQRG
                     IDGALKLQLTENLTARGNVAWGQCKGYGLQSGHFLLEAKEIADINSQGGVFCDHMQTL
                     TSSAIVSYRLLERTTFTGQMLFASGLRTSENEDAKTNSSHSPSYTIYNLSIAHVFPLP
                     WEGQKFLLGFDIVNLFDQRYYINQGEGSIGLGVSHAGMPRSFFFRGQWFF"
BASE COUNT         3249 a         4611 c         4387 g         3244 t
ORIGIN      
        1 gccgttggat cggtacgaag cctatcagga gagccgccgc aaacaggaag aggccacgcc
       61 tgagcaaaac cccgaagacg acaagggcca gccgctcccc tatcgcctgg gtacatccgt
      121 gtcgaactac tggattccgc tgctccccgt gatggatgga acgtcgctca agctcaagcg
      181 cggcaccatc cctgatttca acgccgggga aaggccgcgc ctgatcgagc cgatgggact
      241 gatactcgaa ccggggcgcg agttactgct gcaggaagaa gaggtgccgc gcgaaggggc
      301 cagagtcacg cgtacgtatc aatacgccag atggatcgac ggctcgacgc atctctggat
      361 cggccggcgc aaagaaccgg gccgcggcga aggctccagc gggctgcagt tcgatgtnnn
      421 nnnnnnnnnn nnnngaagag ggaggaaggg aattcatagg gacctctgac tcttaatcag
      481 cgcgtgagga ttaaaattga tagatgggcg aaagcagcct gtgctaattc tgaccgcatg
      541 acatcaacgc taccgtcaca aaactttttc ctgtacctaa acatcgatgt attccccgca
      601 gcctgggccg cctatcgcag cactttgatc actccagcga attacggcac accaagcttc
      661 cgaccgatta gctactggcc ctacaatctt ttgaagacca agcgcacgct tcgtcttcaa
      721 aaagaaatag aacttgagct aattcgaaag acgttttact cggctcaagt gtctcgcctc
      781 cacggcattt acctatgggg agatgagcaa tctgcaaaac gaggagagag gtggcgagaa
      841 acagaaggaa accattttca tgccgactac ctggttgaag taggattcac gtacaaccgg
      901 ttgactaagg tagacacaaa ttggattgac gaatacctgc ttcatgacag tactccactg
      961 gatcgaaact gtctggattg gatgcacagt taccggagag gtgagccttg gcctacagct
     1021 gagccgcttt gggaatatat agcggagggg cgcgggttgg tgtacggaac cgctctcaga
     1081 aagcaggctt acgagaaggt aaagaccctc gcacccgact cgttgggtca attagagctg
     1141 gggcgaattg cagtagaaat gggaagcgat ctgtatcaat tgtctccttt cattcggcag
     1201 ctatcgccaa catccttcac agtagatttt catctagaca gccacgatga aaatgaccaa
     1261 ttcatggaaa aggccggaga ccatattaag cggatcatgg aaaaagatcg cactcacata
     1321 aatcatgaag ccttagatct tctgcgcggc gacactagac gccccgatct acgcagcatg
     1381 ggcgtcgctt ttgattgctc agaattttcc atcgaagcga aaacgcttat tgaaagcctg
     1441 gtatccgccc atgacggttc aatatggtca ggtttaaccg agcctcccag tgccaaaaca
     1501 ccctgacaag atctgatcaa agataagctg tgccatcacg aatcctcagc ttccttgagg
     1561 aatgctctac ttactcttgt cgaatagcat ttcggcggaa tctcaatccg gggcgtttgt
     1621 tttgccacgg catcgcgaca agtggctctc atttgcagcg aagggaatgg taccaggaat
     1681 agtttgatct ccctcaaggc gtgcaactcc agcaggggcg attactggaa ccggcccgcg
     1741 agctgctgct ttatgaggaa gtgccacacg aaagcgcggg cgtcatcaga accgatcgat
     1801 cttcgcgctg gatcgacggc acgacgcatt cgcgcctcag cacctccgaa ggaatctcat
     1861 aggaagaacc tgacagccga cacctacgcg cccatgggtt tcctcgggtc tctacattat
     1921 tcatgcctac agacatttct taaatcactg ctacggttac aagacacgat gacgaggctc
     1981 cacctcaaac gcctctatgc gcgttggatc gacggcacca cgcatctctg gatcggccgg
     2041 cccaagaacc cgggccaggg cgaaggatcg agcggattac agttcgattc cgtcgaatcg
     2101 cctggcggct gatctgtaga caagaagact cttcgaggac acactaggca tcacggtcgt
     2161 atcggccata ggcgctcctc tcggtgaaac acttcagaat cgccggttca tcaaagttaa
     2221 agactaagat ctgagaggga agggtgacca tcaggacgcc tgcccagagc ccagtggtac
     2281 ttccgctcag atgcagtgat gggaaggtcg ttaatactgg cgatgcgccg acgcatgagg
     2341 ccattctcat cgaattccca gttctcgttg ccatacgatc ggaaccaact cccagtttca
     2401 tcgtgccact catacgcgaa gcggactgcg atgcgcgccc catgaaatgc ccacagttcc
     2461 ttaataaggc ggtagtcccg ctcgttctcc catttgcggc gtagaaagtt cacaatcgct
     2521 tcccgtcccg ttaggaactc tgaacgattt cgccaccgac tatcggcact gtaggccaac
     2581 gacactgttt cggggttgcg cgtattccaa gcatcctctg ccatgcgtac tttctgggtc
     2641 gcggactcgg catcgaatgg aggaaatgga cgcctaggtt gttcgagagc attcacgatc
     2701 atgacctcct tacgtggttc tcatatatgc aggcgggagc gaatggccgc ggccatcggc
     2761 ttccacgcga catcacctgt aatcagctat ggtagtaacg ctctttgacg atcttgccgt
     2821 cctgccagtc cgttttgacg acttgttcga ggctgatagt ttgcccgttc ttcaatttga
     2881 cggtcagcac cgagtcgtag aagctggtac ttccagaaat agccacggca ccgatggtgt
     2941 agctgacaaa ggccgccacc ccgatcgtct ctctaaattt atccagtcgc tcaatggcgg
     3001 cctgtttgcc cacgacggat gcctggtcgc cttcctgtgt cacgaccgaa tccgcaaagt
     3061 agatcttctg accttcggtc atctgcccct ccttcaggat gcccaacatt tcttctaacg
     3121 tgtccttcac tgacttgctc atgatgattc ctccctctat gccttgcggc gtttcatctg
     3181 tatgacaacc aggaattccc acacaggtga tggttgaaca atcaggatgc cgccaccttc
     3241 tgctgtccct tgcgctagac ccgttgaatc cattgtggac tgagccgcgt tgtcgatggc
     3301 tgcggcattc atgccatctc ccgcctggcc tgcaattatt gataagcact tctgctgcaa
     3361 tcgccgattc actgctacga cgggcctttg gccggcaggc tcgccgtgac tcggcggttc
     3421 gctccgcggt ggcctcacaa cgcatgagcg gcctcgcatt gacggtgccg agccagctca
     3481 actgtgtcgc cgactcgaac gtctccgccc cggatcacga aacaattcaa agcgagcgtg
     3541 ccgccgaatc tccgcacgat atccgtaagc acctgacgat cttgtttcaa actgtcagga
     3601 tcaaacgtcg tcatgatgca gcgcccgcgg agatcttgga ttccgatgat gacctctccg
     3661 atgtgcaagc actgcccggg ccacgagcgc tcagccaaac cctcaacccc accgatgacc
     3721 agattcggtc tcagccgccg accgtcatgc ccgaatgccg caaccgctcc atccgtcgcc
     3781 accaacaacg ggagcacgtc aaagcgatcg ctggagtcgt cgcgtaccag catggcacca
     3841 ggcccaacga tgtcctcaac atccatgcgg acattggggt cgctccacag ccggccatcg
     3901 accaccggct cgccggaccc attaagggtg gcacggtgac cgagcagccg gtagtgcgat
     3961 ctagccgtga tcacatgacc ttgggcatcc tccacatgca cgacacgatc tccttcgata
     4021 ccgagcgcgc cgatgcgtgc gtgttctagc cgttcaccgg ccatcgactt gactggatac
     4081 cgccatattt gctcgacctg catgatgtgc gctcctccca taaaggggac agctgccccg
     4141 tggcgaagtc gtcggggttt caccgagaca attgagtctc gagttcggca attcttgcct
     4201 tcagcgtgcc catcagctct tcgatctctt cgagcgagta tcgtggtgtg atgtacttcg
     4261 gacaattcca gtcgaacgac accacgttga tgaagacgat tcgttctact cgtgctttca
     4321 tgccgggatt ggcgtgttgt accgcgagat ctggatgtaa tcgggcatcc tcgactctgg
     4381 catggcccag gattttcagt ctggcccgat tcgggtaatc catcagaaac aaggacactc
     4441 gatcgtccac agacaggttg cccgtgctca agagttgacg gttcccttga tagtccgcaa
     4501 agactaaggt cgaggggttg agtaggcgta ggaacccttt aggccctccg cgatgctgga
     4561 catagggcca gccggtttcg ctcaccgtgc tcaggtagaa actgtctctg gctgcgagaa
     4621 actccgcctc tccttcgccg aggagatcgc gctcaggagc acccgtgatg ttcgccgcat
     4681 gtccatagca atggtgctgc gcgcgaagga ccgagtcggt catcatcgtc tcgagatatt
     4741 tcatcgccat cggcctgtcc tccatcgaag cgacctgtca gccggtgacc gttgcaggcc
     4801 gatctcttac ctgttcgttc tgtcagaata cgaccgtgtt ccagccttca ttgacatacc
     4861 ggcgtaagct caacaggccc gacgtgccgg ccagcgcatg gtccttcttg agcgggactc
     4921 cgcacgcttc gacgctctcc tttgcgccaa acacatccgc gcagccgcag gaggcgcctt
     4981 gcacgacatc tcgcaccgcg ttgtagagac cattggctgg gtgagagagc ttggtcagct
     5041 cagccggcca acgggtaccc gttccgttga acacgacggc gacttcatcg ccctcctgtt
     5101 tgcactccgc cgccaaggcc agtgcattaa agacacgtcc caatgcttcc tccgagccat
     5161 tcttcggatc tgacatcaca ataatcgctg tcttcatgga ttctccttga tggttgaacg
     5221 gtcgtgataa acggtttgtg atgggctttt gtatttgaat cgccatcgtg ctgagctgat
     5281 atggcaaggc tgatgccaag gcgggtcgcc atcggtagaa actgagaaac tgttgaacag
     5341 atcgagcgtt agatcacagg gcggttcgaa atacggccac gagacggacg aagacttcca
     5401 cgacatatcg tggagctacg aaatacggga ggacatggtc tgtcggatat gtgtttagga
     5461 agaggggcgt ggtttcttga tcccaagctt ctgcatgcga gatcgcaggg tattgggatg
     5521 aagggccagt ctcgtcgaag cgccctgctt gccttcgacg acccaatcca cttcctggag
     5581 gacgcgggtg atatgggtcc gttcaacttc ttctagcgtg taggacgtgt ctgaggttgc
     5641 aactcgatcc gattggagga gcacctcctc aacctgcagg acaggtcctg gtgagaggat
     5701 ggcggcccgt tcgatgacat tctccagttc tcggacgtta ccgggccagg catagtgtat
     5761 gagacgatcc atggtctgtt gggagatctg atcgagcctt ttcccaaact tcttggacaa
     5821 gcgtccggca aacgaacgga cgaagagcgg aatgtctgat gagcggtttc gcaagggtgg
     5881 cagttcaatc gggaacacgt tgagccggta gaggagatcg gagcggaatg aaccggcctt
     5941 cacagcagca gggaggtctc ggttcgttgc cgcgatcact cgaatgttca cttttgtaga
     6001 ccgaccgctg ccgacccgct cgaactcacg ttcttggagg acacgcaaga gcttcacttg
     6061 cgcgtccaac ggcaattcac cgacctcatc gagaaagatg gtccctccct ccgccaactc
     6121 aaagcggccg atccgtttgg ccagtgctcc cgtaaaagag cccttttcgt ggccgaacaa
     6181 ttcgctctcc accagattgg cggggatcgc gccgcaattg acacgaacga gcggtctcgc
     6241 gctgcggggg ctgaggtcgt gcacggcccg ggcaatgagc tccttcccgg tgccggtttc
     6301 gcccaagagc aagaccgtcg aatcagtcgg agccacttgt tcgaccttcc tcagcacggt
     6361 cttcatcgct tggctctcac cgatgatttc accaaaatga tgatcgagtt tgatctcttc
     6421 ccggagatac atattttcgg cctgaagccg gttggtgagt tgctcgacct ccgtaaacag
     6481 ctgggcgttc ctgattgcgg tggctgcttg atgggcaaac gtggcgagcc ggtcccattc
     6541 ctcatccgcc agggggcgtc gtccaaacat cgcgatgacg ccgagcacgt ccccgcgaaa
     6601 gacgaggggg tacccggcaa aggagtggag cccatggtct ttcatccatt gcttgttcgg
     6661 gagtcgatcg tcgccgagca cgtcgttggt ctgcatcgga ccgaaccctt gggcgatctt
     6721 gccgatcttc agcgacccca gggggatacg gcggtattcc ccatcgaggt ttgaatagag
     6781 tcccgcactc gccctgagat gcaaacactg ggtgcggtct ctgcagtcag aggccttgta
     6841 gcagccggta cagagatcgc caggaccgcg gagccagata cgggcaaacg ccgcgtccag
     6901 gtcatccaca agaccttggg tgatcgtcgt gaggacgatg tggagatcca aactcgacgc
     6961 catctgcatg gcgatgcgat ccagtgcgct atgagaatgg gaagaaggcg aggaagggga
     7021 accgtccatt gtgggatctc cgagccgggc atggcactgc gtggaggacg ataagtggct
     7081 gataacggat tgtcaataag caggggaaac ggggtcaacc cactcgagaa atgcacggtt
     7141 caggtagggc ccgccgccac gaggagttcg caggaaggga ggaccctgct ccagggcctt
     7201 cagccgcttg acgtctggca cggtcatgcc gccgaacttg cttcgccaga ggtagtcgtc
     7261 ggcgtcggag agtccatgtc gtcgacacag atctttgatc gagacctcag cctcagcctc
     7321 ccacaaacac ccaatgatct ggaccacgga caaccgcttc ttctgtgcca atctcctttc
     7381 ctgaccggat tggactctaa ttcatggtgc gactcaatca agggggaagt cgcaggaatc
     7441 ccgctcgccc ttgttcttcc tgtcccgcga cgtcttccac gcggaacctg gcggaccgtc
     7501 acaacgacat cctggccaaa acgcgacagg aactgcacga gacgctcaat ggaaactctt
     7561 gccagtgagc gacgttgtgc caaaacgatt ttggtgagcg atggccggcg tcacacagat
     7621 ctggaatagc ctgtgatgca ggcctcagca cctgtttgac atgatcgtca cagggcgata
     7681 tcgtgagaca tgttcgctcg gctattcgca acactctcgc tcatgacgct cgcagtcctg
     7741 ttcaaacgcc tcgtcacacg caaacctccg tcacgcccct accgcaaggc tgaatccaag
     7801 tggcccagcc gaagctaccg cgaaccttcc tgaattctgc tgcggcacca gggtgagtca
     7861 tcaccgacaa cggactcaga ccgatatccg tcgcgacgga ctctacccct gcaatcggct
     7921 ccagagcttc tcgagcgttt tgacgaaggt tttacattct tggtcggtga ggtctgaaaa
     7981 cagttccgcg acctgctctt tgtattccgt gcagcgcggg gagagctcgg tcatgacttt
     8041 gtccgagagt tcgatcacgg tggcccggcg atccgtcggg tgtctggttc gcacgaccaa
     8101 tccgtctttc tcgagcgaat cgacgagcgc cgtcacgttc gttgccgtca cgcccaattc
     8161 tttcttgagc tcgctcataa tcctcgggcc gcgctcatga atcgaaccaa gaatgcgcaa
     8221 ccgttgaggg gacaacccct tctccaccaa tagagattcc gaccaccgtt gataggccgg
     8281 ggcaaagctc cacaacagtt cgaccagccg gtgtcgtggc aacttcgaga tgcagggaga
     8341 gacggatgac ttccccttgg ccctgggggc ttgcattttc ttgatacgct tcatcgtgtg
     8401 tcacggtact tcaaacaggg gcaacattca atgataaatt gttcataacc tgatcattca
     8461 tatattgact atgtttcctg ccatgccgga tactgcgctg catggaaatc gtcaagatac
     8521 acattcactg acattcttga tctagggctc tcgttcacaa agaatattct cagtaggtac
     8581 accatgatca ctctccgata tacaccatgg ctgcttgcat tcctgctgac cgtgacaggc
     8641 tgcaacagcg agcaggccag cagcggcgga caagcgccgc cacctgaagt cgtggtcgcg
     8701 caggtactct cgaagtcggt gcaacaatgg gatgaatata ccggtcgcat caccgcgatc
     8761 gacacagtcg aattgcgacc gcgcgccagc ggctatgtgc agcgagttgc ttacaaggaa
     8821 ggtcaggatg tgaaacaggg cgatttgatg ttccagatcg accctcgacc ctaccgcgca
     8881 gcgctggaca acgccctggc tcagctggca cgcgcgcgcg cggcgaagcg gctggagacc
     8941 atccgcaaca cacgcgccca gtcactgatt cacgatgacg ccatctcgca cgaggaactt
     9001 gacttgcgtc gtgccgcgca agagcagagc gcggcggatg tccaggccgc cgaggcggct
     9061 gtggccaccg ccaagctcaa cctgtctttc accgaggtgc gcgccccggt ctccggacgg
     9121 gcaagccgag ccctcttgac ggtcggcaat ctggcgaccg ccgacgaaac cctgctgacg
     9181 acggtggtgt cgcaaaatcc gatgtacgtc tatttcgatg ccgacgaaaa cagttacttg
     9241 cgctacaaag aacaggagcg caagagcgag cgcacagctc aggacaacgc cgtacacgtc
     9301 ggcctcgcca acgaaaccgg ctatccccat accggcaggg tcgactttct cgatactcag
     9361 gtgaatccca ctatcggaac cgtgcgcgct cgcgcggtgc tgcccaatgc cgaccgcatc
     9421 ttcacccccg gcctcttcgc gcgtgtgcag ttcgtcagcg gccagaaggc acaggtgctc
     9481 ttgatcgacg ataaggccat tctcacagac caggaccgaa aatacgtcta tgcggtagac
     9541 agggacggca aagcgcaacg caaggacatc gtgtcgggtg gcatggtcga tgggctgcgc
     9601 gtggtccaat ccggcctcgc gccggacgac aggattgtcg tggtcggcct gcagaaaatc
     9661 ttttatcccg gcatgccggt cattcccgcg gaggttccga tggacaaatc gtcgacaccg
     9721 gccgcgcccg cagccatggc cgagaaatag ggagccccgg ctgtggactt ttccaagttc
     9781 ttcatcgatc gcccgatctt cgccgcggtg ctgtcgatcg tgatcttcgc cgccgggttg
     9841 atcgccatcc ccatcctccc gatcagcgaa taccccgaag tcgttccgcc cgcggtcatc
     9901 gtgcgcgcgg tctatccggg ggccaacccc aaggtgattg ccgaaaccgt gtcgacgccg
     9961 ctggaagaac agatcaacgg cgtcgagaac atgatgtaca tgaagtcagt ggccgggtcc
    10021 gacggcgtgt tgcagatgac ggtcacgttt cgacccggca ccgatccgga tgccgctcag
    10081 gtgcaggtac agaaccgcgt cagccaggcg ctgtcgcgac tgccgtcgga cgtggtcagt
    10141 ctgggcgtga ccacgcagaa gcagtcgccg acctttctgg taatggttca gctcctgtca
    10201 cccgacggga aatacgatgc gctctacctg cgcaactacg ccaatatcaa ggtcaaggat
    10261 gaactggctc gcctgcccgg tgtcggccag gtgcaaattt tcggcagcgg cgattatgcg
    10321 atgcgcatct ggctgaaccc ggacaaaatc gcctcgcgcg ggatgaccgc gggcgacgtc
    10381 gtcgccgcgg tgcgcgagca aaacatccag gtctcggccg gacagcttgg tgcggaaccg
    10441 atcgcgaaag gcaccgactt tctcatctcc atcaatgccc aaggccgcct gcgtacggcc
    10501 caggagttcg gcaacgtcgt gctgaagatc ggcgccggcg gcgaggtcgt ccgcctgtcc
    10561 gacgtggcac gcgtcgaact gggcgccaac gactacacct tgcgcggaca gctcgacaac
    10621 caggatgcgc cgccgatcgg catcttccag gcccccggcg cgaacgcgct gaccgtccgc
    10681 gacgcggtca tcgccaaaat ggaagaactg aagacgcgtt ttccgcccgg ccttacctac
    10741 cgctcggact acgacaccac ggtgttcgtg cgcgattcca ttcaggcggt cgtgcagacc
    10801 ctgatggaag cgatcctgct cgtcgtgctc gtcgtcattc tgttcctgca gacctggcgc
    10861 gcctcgatta ttccgctcat cgccgttccg gtctccgtgg ttggcacgtt cgccgcactg
    10921 tacctgttcg gtttctcgat caataccctc acgcttttcg gcctcgtcct cgcgatcggc
    10981 atcgtggtgg atgacgcgat cgtggtggtg gagaacgtcg aacgcaacat cgaagaggga
    11041 cgcactccgc tccaggcggc gcatcaggcc atgcaggagg tctcggggcc gatcgtcgcg
    11101 atcgcgctcg tgctctgcgc cgtgttcgtg ccgatggcgt tcctgagcgg cgtgaccggc
    11161 cagttctaca agcagttcgc cgtgacgatt gccatctcca cggtgatctc ggcgatcaat
    11221 tcactgaccc tctcgccggc cttggcggcc aaactgctca aaggccacgg cacaccgaag
    11281 gatgccctct cccggtggat cgagcgtctg ttcgggtggg tgttccgacc attcaaccgt
    11341 tttttcaagg ccagttcaga ccgctatcag ggcaccgtct cgcgcacgtt gaaacatcgc
    11401 ggtgccgtgt tcgccgtgta tgcgctgctg ctgctcgcaa ccggtctcat gttccaagcg
    11461 gtgccgcgcg gattcatccc gactcaggac aagctgtttc tgatggccgg agtgaaactg
    11521 ccggaaggcg cctcgctcga gcgtacggat gcggtcctgc gccaggtcgt cgcgatcagc
    11581 aagaccgtgg acggcatcgc ccacgtcgtg gccatgacgg gtttgaatcc tctgcaattc
    11641 acgaatacgc cgaattacgg catcgccttc ctgatcttga aaccgttcga cgagcgtcat
    11701 cgcagcgcga aggaaatcag cgaggagatg aatcaaaaga tctccgggat caaggaaggg
    11761 atcaccttcg tgctgatgcc gccaccgatt ctcggactcg gcaacggagc aggctacgcg
    11821 ttattcgtgg aggaccgggc cggcctcggc ttcggggcgt tgcaaaatgc cgtcagcggc
    11881 ctacagggag cggtcatgca gacgccgggt atggggtacc cgatctcggg ctatcaggcc
    11941 aacgtgccac agctcgacgc cgaagtggat cgcgtcaaga ccaagacgca gggcatcgcg
    12001 ctgacggagc tcttcaacac gctacaggtg tacctcggct cggcctatgt caacgacttc
    12061 aatctgttcg gccgcacctg gcgcgtgtat gcgcaggccg acggcgattt ccgcggcaag
    12121 gtagaggaca tcgccaacct caaaacgcgt aatgaccgag gcgaaatggt cccgatcggc
    12181 tcgatggtga aattcagcca aacgtatggc cccgatccag tgctgcgtta caacggttat
    12241 ccggcggctg actttatggg agaggcagac ccgaccaaac tgtcctcggc acaggcaatg
    12301 gacaatattc gcgacatcgc cgcgaaggtg ttgcccaacg gcatgaccat cgaatggacc
    12361 gacctgagct ttcaggaggc ctcgcagggc aaggcggcac tactggtgtt cccggtggcc
    12421 atcctgttgg ccttcctcgt cctcgccgcg ctgtatgagt cctggaccct gccgctggcg
    12481 gtgatcctga tcgtgccgat gtgcatgctc tgcgcgctcg ccggcgtcaa gctcaccggc
    12541 ggtgataaca ataccttcgt gcaggtgggc ctagtcgtgc tgatgggcct ggcttgcaag
    12601 aacgcgatcc tgatcgtcga atttgcgcgt gagttggaac tgcaaggcaa ggagatcgtc
    12661 gaggcggcac tcgaagcctg ccgcctgcga ctgcgcccga tcgtgatgac atcggtcgcc
    12721 ttcatcgcgg gcacgattcc actcgtgctc tcgcacggtg ccggcgcgga ggtgcgctcc
    12781 gccacaggcg tcaccgtgtt cgccgggatg atcggcgtga ccctattcgg cttgtttctc
    12841 acaccggtgt tctacgtcgg cctgcgcatg ctgtcgggcc gcaagctggt caggcacgat
    12901 gaatcaatgg aacacggcat ggtggaaggc gcgtataggc aggtcgatag tccccgttga
    12961 gtgccccgtg attactgacg gacaccctgt atctcttcgc actgcggatc gcaaagatat
    13021 cggccatgag ctatcagctc ttcgagccat tactgcgatc tgaagtgaat ccgcacggtg
    13081 agctcgccgt cgatagggtc gtcgcctttg agttgcgggg cgaaggtcca tttgttcaag
    13141 gcgatgatgc cggcgacggt cagctcccgg tgtttcgccg gttccaatac caccaccgtc
    13201 acctgcgcct ccttggagac aagcaagcgc gccttcatcc aatcgtcgag cggcttccca
    13261 tccaacgacg cgggtatggc cggccagggg gtggacttcg gcaccggccc cacctgctga
    13321 tccttgttca gcggcagacc gtgcacgtgc acttccggca attcgatgat gtccgcatcc
    13381 aacgcatggt ccgcctcatg cgtcgcctca tctcccgtca cggccaaggc gtatgcaggc
    13441 atcaagcaca acatacagag cgctaccatg ggcactgtca gctgtcgaat tccatcgatc
    13501 atgttcatca tattgtcttc caaccaattg cgggatgatg ctcaaacggg ccgacctcag
    13561 tgtagcagtt tctgatgtgc tcttatacca tccggccggc ccggcgacat tccccctcgc
    13621 tccgaaacag atgcccaaca aggttgtcca gcaaggctgc tagcgacgca tgaatcgacg
    13681 gcgtcactct tccccagtcg aactcggatg ctcaaacggg tcatccagca aggccgcagg
    13741 cgacgcacga accggaggcg taccctcaag ggtacgttga ggattcgtgc gagccgagaa
    13801 cgatgctggt gactcgtttc agcatccggt taaaagaacc actggccgcg gaagaagaag
    13861 gacctcggca tccccgcatg cgacactccc aacccgatac tcccctctcc ctgattgatg
    13921 taataccgct gatcgaacag gttgacgata tcgaagccga gcaggaattt ctgcccttcc
    13981 catggcaagg ggaacacatg cgcgatcgag agattgtaaa tcgtgtaaga cgggctgtgg
    14041 ctggagttcg tcttggcgtc ctcattctcc gacgtgcgca gtcctgaggc gaacaacatc
    14101 tgccccgtga acgtcgtgcg ctccagcagc cggtaactca cgatcgcgga actcgtgagg
    14161 gtttgcatgt gatcgcagaa caccccgccc tgcgaattga tgtccgcaat ctccttggcc
    14221 tccaggagga agtggccgga ctgcaatccg taccccttac actgtcccca ggccacgttg
    14281 ccgcgcgccg tgagattctc ggtgagttgc agtttcaagg ccccgtcgat tccccgctgc
    14341 cagccacgct cgaacgcaaa atagttcagc aacggcgtgg taccgaactg acccgcatcg
    14401 gacaggaagt tgctgagctt gtaccagccc gtgaattgca acgtggcata gcggttcaac
    14461 gcatggtaac tgccgacctc gaagtagtgc gcccgctcgg cgcgcaccgt gttgttggtc
    14521 acatcttccg gttgcgccct ggtcccgagc gtattgagct tggcgaagga aatggcttcc
    14581 agattggggg gcgtgaacat gcggccgtaa aacacgtgga acacgttcga cggattgtac
    14641 ttgtagctca cgccgatgcg cgggctgacc tggccctcgt tgcgttgata ttgcacgatg
    14701 tcgccgcgga gcccgaggtt gaacgtccac tggtcgttcg ggctccactg atcctgaatc
    14761 cagaactcct ggcgatagcc gatctggcgg ttgtcggcat tgagactcag cagatcaccg
    14821 gtcggattgc cccccccgtc gtcggcaaac gtaaacagcc gggtcttgtt caccgactgc
    14881 gtccggtcga gctggaaccc ggctttgatg agatgttgct tgctgtgtgt gtaggtatag
    14941 tcgaatcgca cgccggtcga ataggcattg cgatcctgat cggcggctga aaacggctcg
    15001 gctggatcgg ccgtataggc cagcaggttc agcggatcgg tcttgaacgt ggcacgcgta
    15061 tgtcgcatgt agcccgacag gctgaagaag ttgttggcgt tcacatcgtg ccgccaaacc
    15121 atatgaccgt attggttgtt ttccttctga aactcgtcga tcgcctgcga ggccacgggt
    15181 gagaacccgg ggcgactccc ttgcaatagc ggcaacatct gcccgctcgg atccagttcg
    15241 ccgcccggtg acgtggggat ctgatacttg gccaccgaat tcaggaacaa ccaggtaaag
    15301 ttgtttctgt tgtcgtgttg atagtcgccg cggataaagg tttggttgcg ctcgctctgc
    15361 ccgtgataga cggatcggcc caacgtcggc ggctcgatgc cccgattggt ggccgtgtgg
    15421 ctgttcaaaa tatagaaccg gaacttttcg ccgatcgtgc cgccgtactc gaaggacgga
    15481 ttgatggtct tgttcgagcc gcccatca
//
LOCUS       DAORRQ010000078        15011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_212_length_15011_cov_21.665619, whole genome shotgun sequence.
ACCESSION   DAORRQ010000078 DAORRQ010000000
VERSION     DAORRQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15011
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212_length_15011_cov_21.665619"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..835)
                     /locus_tag="PK224_17760"
     CDS             complement(62..835)
                     /locus_tag="PK224_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17647.1"
                     /translation="MRLGVLIGAIMLVGWVAGCGSSAKLMSGLSIEELKPVPGILPSA
                     RLIRGTISSFSGDRVEVETGERAPRYLSLEAGGDEARGLQPGGAVEIMVNDRDEIVAY
                     QRPADQPPTKVFRGSLGRSFNPQKERVTIRLDTGRDVGFYARRVAMEKLAALEVGETA
                     DFAVDRAYLLVDVFSLGKPRELALESSSRAPHHRLQGAFVTIGDGRMRLRMKIDDIQS
                     FPVRPLLQPAIEQLAPNELVSVYLDAQGQVIDMVRATRR"
     gene            1027..2634
                     /locus_tag="PK224_17765"
     CDS             1027..2634
                     /locus_tag="PK224_17765"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRB17648.1"
                     /translation="MTPPPVETAAVDSFETTLEGGTSQIGLFLILILLTIGGIYVAGE
                     LTSDLSDVRASSVWPFILLGFALLIALGFEFVNGFHDTANAVATVIYTHSMPPHLAVA
                     WSGVWNFIGVMLASGAVAFGIVALLPVELILQVSAGAGFAMVFALLIAAILWNLGTWH
                     LGLPSSSSHALIGSVIGVGITNQLMAAGGTNTSGVDWTQALNVGKSLLLSPLIGFSAA
                     AFLLLIAMKAVPVEKLYKPPRGKTPPPLWIRGLLILTCTGVSFAHGSNDGQKGMGLIM
                     LILIGTVPTVYALNRTVDISYTPAFVEASSKSEAVFQRYSKATTTPADPRQIVAEYLR
                     THEVRPDTLPALRALSGIVRTQIASYATVAQIPTEQTSNVRNDMYLLGESLRWMDKTR
                     EPAFARADRDILLHYKSMLDNATKYIPVWVKAAVAIALGLGTMIGWKRIVVTVGEKIG
                     KTHLSYGQGASAELVAMTTIGAADLFGLPVSTTQVLSSGVAGTMAANRSGLQWQTVRH
                     LALAWVLTLPASIVLAGTLFWFFRHFA"
     gene            complement(2732..3466)
                     /locus_tag="PK224_17770"
     CDS             complement(2732..3466)
                     /locus_tag="PK224_17770"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HRB17649.1"
                     /translation="MIARRPSGYRLIAVLGLACLSLSDPARAEHESLPPDYPDSLERE
                     LASLQSRIATEGGKVDRLIAGAELYLNIADDLFEDEAQKRLAYEAAADMAKRALQMEE
                     RNAQAHFLYAAARGSAERLKGIANAGLVLGEIREHVSRAIELDPGHAQALQMMGGLYA
                     ELPWLLGGSEKEAESYLRRAIAADGRYTNAHLILARLLIKQGRSGEARAHLDAVLQVE
                     HPHYPYAWRRRFRPEAERLLKALPAS"
     gene            complement(3463..4518)
                     /locus_tag="PK224_17775"
     CDS             complement(3463..4518)
                     /locus_tag="PK224_17775"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRB17650.1"
                     /translation="MAVHDVAAALLILLYAALFRWAFRVLPRDGWQVLATVPQARRPD
                     GRWRGLNLTYYGAFTASAVVVAVALAIVLLGSVAVPFDAILWLSVILLGACVPSAKIL
                     ARLIEGKSNTFTIGGASMFGLFLAPWIVAALNAGFTASEDDVLPVTAVLATVAIAYAF
                     GEGTGRLACLSFGCCYGAPLNRSHPWIAGLFVHHHTAFAGETRKAAYESGLESVPLVP
                     IQAMTAVVSVAAGMVGLWLFFHGRMAAAFVFTVVVTQLWRVGSESLRADYRGGRKVSW
                     YQVLSLGGIAYAAFLAFWLADVPSVPPDALRGIRLLWQPGVILCLQGLWLGMFFFTGR
                     SRVTTATLSMHVVKDQI"
     gene            complement(4505..5533)
                     /locus_tag="PK224_17780"
     CDS             complement(4505..5533)
                     /locus_tag="PK224_17780"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRB17651.1"
                     /translation="MIAPHQYIERETRLIKTERFCGDRWLNWAYRALWEDASWLMKAL
                     TSQRTTQVLGFFNYDRPFTATQTAVSKCALAMEIDLSECLESRDRLNTLRRLFQRRLR
                     YWQVRPMEEDLRAVVSPADARLLLGSFTEDSALFLKGKFFDYDELLGIDRSRWLRLFR
                     QGDWAIFRLTPDKYHYNHSPVSGRVLDCYDISGRYHSCNPGPVVVAATPYSKNKRVVT
                     IIDTEVPGGSRVGVVAMIEIVALMIGHIDQCYSAVAYDDPRPIQIGMMIEKGRPKSLY
                     RPGSSTDVLLFEPGRVAFCEDLVRNRFRPGVKSRFSQGFGRALVETDVRVRSTIGWRR
                     EGSPDGRS"
     gene            complement(5530..7935)
                     /locus_tag="PK224_17785"
     CDS             complement(5530..7935)
                     /locus_tag="PK224_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17652.1"
                     /translation="MSRTTTISSNFPLRPAEPRDVLEKEAAQFLTGLPVLPFSHDDIT
                     AGSESELQAAVCGISEDVDLARTIQSSNYLHNIRERTAAGESPRIVIRQLQEFLASPD
                     ATVWENSWVRFPRRLLNRTADQLFEGDLAADKRHAKGPLRADAHRYLLTEQGEEWVRL
                     PISYLLKLTLAQVIEEFERDAPLLAIEGRRLLNHFLNDNSSPETFSFYVSPMDRAQGM
                     GTGIARETAVRFLFTHLLALYANRQFGLQESGQQVLVYASPHPPLRQRRLNGLVSDAF
                     YRELFMNPCLSGWERGEEKSQYMGLCHEVLSRSQLNAVVRLKDAGIITRNLVVLPSTS
                     NISLANNGTHLSLGSRILTQALQSGGKAFSAAHEKVVGDLVIKIVEHFLPLFAGTYSA
                     APYRMNFRDFHPETALGFLPHELDFTHLRMLWRRWKKKAGLSVFGRSITPVGPLWVDR
                     ALSALFHLRGDFLPDFRLIDYLVSLLSTDQSPALNGIQGNDLRLKRDLGQLGVFDERM
                     SLYLFYKLREESVMGFSGFEGRQHSLFSNHLDDFADAASLQALVTALAFRYIAAGSVT
                     HDDIPDDPFTESERRQCIFASAIGVGTVNVREGGPNRFLARILSRTQKTRVSRRYAGN
                     LRVRVDDYRLALLATLEEDGAELIEAFQFSGHLGRLRQRLLEPESHSTAGKLTRGILD
                     SIGAESPMQLSGEEFAQAAEAYYRRQLRTQYVREGLGCVREDLQAIDEGRSETDRRYR
                     GTATAVIGPRSAADVLAETEQELLSETADIPALRRCLALCLLTITRAGAASLSRGGRS
                     LAS"
     gene            complement(8173..8856)
                     /locus_tag="PK224_17790"
     CDS             complement(8173..8856)
                     /locus_tag="PK224_17790"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRB17653.1"
                     /translation="MATIKAVMFDFDGVLFDTEPLHFEMFRQVLRMEGVTLAPDRYHA
                     RYVGLTDQACFRAVLTDSGWTAVPAETLDRLVQRKTGLMQEALRKTLPVLSGVREFVA
                     AVTETCRTAIASGALRQEIALCLELAGMTSMFEHVSAAQDVGQGKPDPALYLHALGAL
                     NRRSPLRAHECVAFEDTPHGIEAARKAGMRCVGVATTLPESRLVQADLVVPSLHALPL
                     SQVLAHLSP"
     gene            9083..10201
                     /locus_tag="PK224_17795"
     CDS             9083..10201
                     /locus_tag="PK224_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17654.1"
                     /translation="MAPLDAPPLSIEQGTCYALFAYDIGLAINLDEAERHVTAIKERG
                     RIRHKARAPHYFEYRPAPLRLTQETVALAFGDYRSSEAVEVMLYDFGAVTVIYRIPIN
                     GPFDGLLSLSESLYENETLLTESRHRLDQLVRDIFPAIERPSTSKEVEDYLIFSIEHC
                     TPSAIQTFGAPYDPLFAHVLRSERTRLSDQEIHEATSCRISFSRDDTALIDWNAALVF
                     GQDMDDVRAVLEFVNVELLEMRALDEQLDTALDQGYDALTRKTGLTSWLPGSHEVALT
                     HIGQLQVDSAVLFERVANTLKLLGDQYLARVYRLASQRFHLEAWDASIIRKLQTLESI
                     YGKMTDRATTRRMEVLEWIIIVLIAISIAISFLPFTSH"
     gene            10329..11555
                     /locus_tag="PK224_17800"
     CDS             10329..11555
                     /locus_tag="PK224_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17655.1"
                     /translation="MNDQRRSDPVRGTIHAGWLRSLVGLLAVLSLAGCLSPPTLNRAV
                     LAYDEAITDAISKQLLINIARAHHHEPIHFTGVANVAATFDFRISAGATPALTGEHGR
                     TLVSLFGGSIAENPTISITPIEGEEFTKRILAPFQESKLTLLLRQGVDIDLLLRLMAK
                     ELRLKHKGEEVAYRNSPSDKDGYDMFRKVVLHLSAIQDANHLYAEALTFERTWTIPAE
                     SVTAEGFATLEQQYLITYQSETRTYTLRKPVSGRILITNYDPATLPPAERVRLHETAD
                     QRPVNDVSFDIRAGHFGGEWPLQGDFRLRSFNAMLNFLGHAADEDREYAVEKDARTPP
                     VAENPVHTMDLLILDHTPDEPDLAVKSHGRYYAINATGPQARWNREAFKLLSQLFQMT
                     VTDVPRTGVPSITIAK"
     gene            11707..12732
                     /locus_tag="PK224_17805"
     CDS             11707..12732
                     /locus_tag="PK224_17805"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRB17656.1"
                     /translation="MHRNVISRSLLWQWTAFFISLCLALPSWADEVHLQNGDRLTGTI
                     LKMEERVLTLQTDYGGEVKIDWGKVESLKSDGTLKILVPGESHDALRDFLYGAQALRE
                     TRELGPESPVPLADITAINLEPLRLTGTVTVGGNSTSGNSSTKAFNSAARLTIQAYRQ
                     RLLLEGKYNYGQAGELVTARNSLASVKHNYFLSKQIFIESFGMLEKDTLQNLQLRSTI
                     GSGLGYQFYESARTTLSLSLGLAHVSEHFTNSPNTQTPSGRWSLRWEHAVWPDRVKVF
                     HRHEGFYDVNAGNAFRINADQGVRITVYKNLFFNVEYDLRLNTQPAPGREKIDESLIF
                     GVGYEFK"
     gene            complement(12760..14094)
                     /locus_tag="PK224_17810"
     CDS             complement(12760..14094)
                     /locus_tag="PK224_17810"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRB17657.1"
                     /translation="MIPPTVDLCIVGAGAAGLAAGIFAAERNPHLTIELLDGAKTIGA
                     KILVSGGGRCNVTHDVVTPDDFFGTRHLVRNVLAAFPVRDTIQWFASLGVDLKCEETG
                     KLFPVTDKARTVLEALLARSRALGIVLRPGHRVTDIARLDDTQGANKPEPARFVIRHQ
                     EGETFARRVILATGGRSLPRTGSDGSGYGLARRLGHRVTPTVPALVALVLDDRCFHSG
                     LSGLSQEVEIQTVLQGRPVDRRIGSLLWTHFGISGPVVMDASRFWCLARERGEAAEVY
                     GNFLPGRTTEQAREWFLHQTAKNPRRSLLKLLTDVLSERCAETLCRHVEGDPQQACAQ
                     VSRNIRDRLLSALTRFQFPVVRDRGWNFAEVTAGGIPLEEVNFRTMESKVVPGLYLVG
                     EVLDCDGRIGGFNFQWAWATGRLAGRAAAVTPIAGSSEAGGNGEQGERAAGK"
     gene            14219..>15011
                     /locus_tag="PK224_17815"
     CDS             14219..>15011
                     /locus_tag="PK224_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRB17658.1"
                     /translation="MIQSRDKFQPSISLSRPMAGSTLRIALLHLAPIPGALAKNRHLI
                     AHAITKAACLGAAWIITPELAVSGYTFADTLGTDWIAPQPDPWMEKVRLFAARKRVAI
                     FLSHPERDPQSGRLYNTVFAITPDGRLAGSHRKINALRVGSEAWSTPGTETTVFPMEP
                     LGNVGMLICADAFSPGIANSLKAKGAQILVSSAAWAPGLHGPNGEWERCTKDTGLPLF
                     VCNRTGADRTLDFRKADSVVAQDGRRLLSMSSERSAIFLIDWDLKA"
BASE COUNT         3141 a         4953 c         4167 g         2750 t
ORIGIN      
        1 tcgcctgaat gactggctgc tcacccggat cgtgaagctt cccccgcgcc gctgacgtcg
       61 ttcaacgccg ggtcgctctg accatatcga tcacctgccc ctgcgcatcg agatagaccg
      121 ataccagttc gttgggagcg agctgttcaa tcgcgggctg cagcaacggc cgaacgggaa
      181 aggactgaat gtcatcgatc ttcatcctga ggcgcatccg tccgtcaccg atcgtcacga
      241 atgcgccttg caggcgatgg tgcggcgcgc gtgacgaaga ctcaagggcc agttcacggg
      301 gcttgcccaa cgaaaataca tcgacgagga gatacgctcg atcgacggca aaatcggccg
      361 tctcgccgac ctcaagagcc gccagtttct ccatggcgac cctgcgcgca tagaacccca
      421 catcccgccc cgtatcgagt cgaatcgtca cccgttcctt ctgagggttg aacgatctcc
      481 ccagcgagcc gcggaagact ttcgtgggcg gctgatcggc aggtcgctga taggccacaa
      541 tctcgtcgcg atcgttgacc atgatctcca cggccccacc cggctgcagt ccacgcgcct
      601 cgtccccgcc ggcttcgagt gacaggtagc gcggagcccg ttccccggtc tcaacctcca
      661 cacgatcgcc ggagaaactg ctgatcgtgc ccctgatcaa cctcgcgctg ggcaggatgc
      721 ccggcaccgg cttcaactcc tcgatactga gcccggacat gagttttgcc gagctcccgc
      781 aaccggcaac ccagccaacc agcatgatcg caccgatcaa aactcccagc ctcatgtgcg
      841 tcgcgtccat tgtccctccg gctttcttcc gtcggatgat aaaacaaacc cctgcccgga
      901 ggcgagcgtc cgtcttccac agttaacccg atcttaatcc ggctcacagc gaggtgtaac
      961 gacatcctgc cagactctca ggccgttaac ggttcggcag cggctctccg cagaaaggaa
     1021 ccctgaatga caccgccccc cgtcgaaacc gccgctgtcg attccttcga gaccactctc
     1081 gaaggaggaa cttcgcaaat cggcctcttc ctcatcctga tcctgctcac catcggcgga
     1141 atctatgtcg ccggtgaatt gacctcggat ctctccgatg ttcgcgcctc ctcagtctgg
     1201 ccgtttatcc tgctcggctt tgccctgctg atcgcgctcg gcttcgaatt cgtcaacggg
     1261 ttccatgaca cggcgaacgc cgtcgcgact gtcatctata cgcattcgat gccgccacat
     1321 ctcgccgttg cgtggtcggg ggtgtggaat tttatcggtg tcatgttggc atccggagcg
     1381 gtggcctttg gcatcgtcgc cttgctgccg gtcgaattga ttctgcaagt gagcgccggg
     1441 gccgggttcg cgatggtgtt tgcgctgctg atcgccgcca ttctctggaa cctggggacc
     1501 tggcatttgg gtctgccttc ttccagttcg catgcactga tcggctccgt gatcggcgtc
     1561 ggaatcacca accagctgat ggcagccggc gggacgaata ccagcggcgt cgattggaca
     1621 caggccctca acgtgggcaa atcccttctg ctttcgccgc tcatcggatt ctccgccgcc
     1681 gcgttccttc tcctgatcgc catgaaggcc gtccccgtcg agaagttata caaaccgccg
     1741 cgcggcaaaa cgccgccgcc cctgtggatc cggggtctgc tcattctcac ctgcacgggt
     1801 gtgagctttg cccatgggtc caacgacggg caaaagggca tgggtctcat catgctgatc
     1861 ctcatcggaa ccgtgccgac ggtctatgcg ctcaatcgca ccgtggacat ttcatacaca
     1921 cctgccttcg tggaagcgtc gagtaaatcg gaagccgtgt tccagcgcta cagcaaggcg
     1981 accaccactc cggccgaccc gcggcaaatc gtcgcagaat accttcgcac ccacgaggtt
     2041 cgcccggaca ccttgccggc tcttcgcgca ttaagcggca tagtccgcac acagattgcc
     2101 tcctatgcca ccgttgccca gatcccgacg gaacagacct ccaacgtgcg aaacgacatg
     2161 tacctgttgg gcgaatccct gcgttggatg gacaaaaccc gtgagcctgc ctttgcgcga
     2221 gcggaccgag acatcttgct ccactataaa tccatgttgg ataacgccac caagtacatc
     2281 cccgtgtggg tcaaagccgc cgtggccatc gcgctggggc tgggcacgat gatcggctgg
     2341 aaacgcattg tggttacggt cggagagaag atcgggaaaa cgcatctgtc gtacggccag
     2401 ggcgccagcg ctgaattggt tgcgatgacg accatcggcg cggcagatct cttcgggctg
     2461 ccggtcagca cgacgcaggt gttgtcctcc ggcgtggcgg gaaccatggc cgctaatcgg
     2521 tcgggattac agtggcagac cgtccggcat ctggcattgg catgggtcct gactctccct
     2581 gcgtccatcg tactagcagg aaccctattt tggttttttc gccattttgc atgacccgcg
     2641 cggcggttta tgggccaaac attcggcaca ggcactcatc gaggaagccg gcgccaaccg
     2701 gccgttcggt cttcgactgc cgagggatcg atcaggaagc gggaagcgcc ttcagcaatc
     2761 gttctgcttc cggccggaat ctgcgcctcc aggcgtaggg ataatgtgga tgttcgacct
     2821 gaagcacggc gtcgagatgc gcgcgcgctt cgccggatcg gccctgtttg atcaggagtc
     2881 gggccaggat gagatgggca ttggtataac gtccgtccgc cgcaatcgcg cgccgcagat
     2941 acgattcggc ctctttctcg ctaccgccca acagccaggg caactccgca tagaggcctc
     3001 ccatcatttg cagcgcctgc gcatgccccg gatcgagctc gatcgcccga ctgacatgct
     3061 ccctgatttc ccccaacacc agcccggcat tggcgattcc tttcaagcgc tccgcgctcc
     3121 cgcgagccgc cgcatacaga aaatgggcct gggcattccg ttcctccatc tgtaaggcgc
     3181 gcttcgccat atcggctgcc gcctcatagg ccagacgctt ttgtgcttca tcctcgaaca
     3241 ggtcatcggc gatgttcaga tacagctcag cccctgcgat cagccggtcg accttgcccc
     3301 cctctgtggc gatccgcgat tgcagggagg ccagttcgcg ctcaagggag tccggatagt
     3361 ccggcggcaa ggactcgtgt tccgctcgtg ccggatcact caacgacaga caggcgagac
     3421 cgaggacggc aatcagacga tatccggacg gtcgccgggc aatcaaatct gatccttcac
     3481 gacatgcatc gacagtgttg ccgttgtgac ccgacttcgg ccggtgaaaa aaaacatacc
     3541 gagccacaga ccttggaggc agagaatcac gccgggttgc cacaggagtc ggatcccccg
     3601 cagcgcgtcc ggcggcacgg acggcacgtc cgccagccag aaggccagga acgcagcgta
     3661 ggcgataccg ccgagcgaca ggacttggta ccacgacacc ttcctgccgc cacgataatc
     3721 ggcgcgcaaa gattccgatc cgacccgcca cagctgcgtg accacgacag tgaacacgaa
     3781 tgccgccgcc atccggccat gaaagaacaa ccagagaccg accatacctg ccgcgaccga
     3841 aaccaccgcc gtcatcgctt gaatgggaac cagcggcacc gactccaagc cgctttcata
     3901 cgcggccttc ctggtctcac ccgcaaacgc cgtgtggtga tgcacaaata atccggcaat
     3961 ccacggatga ctccggttca gcggagcgcc ataacaacag ccaaagctca ggcaagccag
     4021 acgccccgtc ccttccccga acgcatacgc aatcgcgacc gtcgccagca cggcggtcac
     4081 cggcaatacg tcgtcttcac tcgcggtgaa tccggcattc agcgccgcca caatccacgg
     4141 cgcaagaaac aaaccgaaca tggacgcgcc gccgatcgtg aaggtattgc tctttccctc
     4201 gatcagtcgg gccagaattt tggccgacgg cacacaggcg cccagcagta tcacggacag
     4261 ccacagaatg gcgtcgaacg gcaccgccac actccccagg agcacgatgg ccagcgccac
     4321 ggccaccacg accgcactcg ccgtgaacgc tccgtaataa gtcagattga ggcccctcca
     4381 acggccgtcc ggacgtcgcg cctgtggcac cgtcgcaagc acctgccacc cgtcgcgcgg
     4441 caagacacga aaggcccacc ggaatagtgc tgcatacagc aggatcagca gggcggcagc
     4501 cacatcatga acggccatca ggtgatcctt cgcgcctcca accgatcgtg gagcgcaccc
     4561 gcacatccgt ctcgacgagc gcccgaccaa acccttgaga aaaccggctt ttcacccccg
     4621 ggcgaaaccg attccggaca agatcttcac aaaacgccac ccggcccggc tcaaacagca
     4681 acacatcggt tgagcttccc ggacgataga gacttttcgg acgccctttt tcgatcatca
     4741 tcccgatctg gatgggcctg ggatcgtcat aggcgaccgc ggagtagcat tgatcgatgt
     4801 ggccgatcat caacgccacg atttcgatca tggccaccac ccccacgcgc gacccgcccg
     4861 gcacctccgt atcgatgatc gtcaccaccc gtttgttctt cgagtacggt gtggccgcca
     4921 ccaccaccgg accgggattg caggaatggt agcggcctga aatgtcatag cagtccagca
     4981 cccgccccga caccggactg tggttgtaat gatacttatc cggggtcagc cggaagatcg
     5041 cccagtcccc ctgacgaaac aggcgcagcc atcgggaccg gtcgatgccg agcagttcgt
     5101 catagtcgaa aaatttcccc ttgagaaaga gtgccgaatc ctcggtaaac gagccgagta
     5161 gcaatcgggc atcggccgga gacacgacgg ctcgcagatc ctcctccatc ggccgtacct
     5221 gccagtagcg caatcgccgc tgaaaaagac ggcgcagcgt attcaggcga tcgcgagact
     5281 cgaggcattc agagagatcg atctccatgg ccagggcaca cttggatacc gccgtctggg
     5341 tggcggtaaa cggccggtca tagttaaaga accccaacac ctgtgtcgta cgttgagagg
     5401 ttaaggcctt catcaaccag ctcgcatcct cccagagggc gcgatacgcc caattgagcc
     5461 accgatcgcc gcagaaccgc tcggtcttga tcaggcgggt ctcacgctcg atgtattgat
     5521 gcggcgcaat catgaggcca gcgaccggcc accccggctg agggatgccg cccccgcccg
     5581 ggtaattgtg aggagacaga gggccagaca gcgtcgcagg gctgggatgt ccgccgtctc
     5641 agacaacaat tcctgttcgg tttccgcaag gacatccgca gctgaccggg ggccgatcac
     5701 cgccgtcgca gtgccccggt atcgacggtc tgtctcggac cggccctcgt cgatcgcctg
     5761 caaatcctcc cggacacagc cgagtccctc tcgaacatat tgggtgcgta gctgacggcg
     5821 gtaataggcc tccgctgcct gcgcgaactc ctcaccggac aactgcatcg gactctccgc
     5881 accgatagaa tccaaaatgc ccctggtcaa ctttccggcg gtactatggc tctccggttc
     5941 gagcagtcgt tgacgcaggc gacccagatg cccagaaaac tgaaatgcct cgatcaattc
     6001 ggcaccatct tcttcaagcg tcgccagaag cgccaggcgg taatcatcga cgcggacccg
     6061 caggttccct gcataccggc ggctgactct ggttttctgg gtcctgctca agatgcgcgc
     6121 gaggaaccgg ttcggtccgc cttcgcgcac attcaccgtc ccgacgccga tggccgaagc
     6181 gaagatgcac tgtcgccgct cactttcggt gaatgggtcg tctggaatat cgtcgtgcgt
     6241 caccgagccg gcggcgatgt agcgaaaggc cagcgccgtc accagtgcct gcaggcttgc
     6301 cgcatcggcg aaatcgtcca ggtgattcga gaacagactg tgctgccgcc cctcgaatcc
     6361 ggagaatccc atcaccgact cctcgcgcag cttatagaac agatagagcg acatacgttc
     6421 gtcgaagacc ccgagttgcc ccagatcgcg cttgagccgg agatcattcc cctgaatccc
     6481 gttgagggcc ggactctgat cggtcgacaa cagggacacg aggtaatcga tcaacctgaa
     6541 atcgggaaga aaatcgccgc gcaaatgaaa gagcgcgcta agggcacgat ccacccacaa
     6601 cggaccgacc ggcgtgatgg agcgcccgaa caccgagaga ccggccttct ttttccaacg
     6661 gcgccacaac atacgcaaat gggtaaagtc caattcgtgc ggcaggaaac ccaaggccgt
     6721 ttccggatgg aaatcacgaa agttcatgcg gtagggcgcc gcgctatagg tgccggcaaa
     6781 cagcggcaga aaatgctcca cgatcttgat caccaagtcg cccacgacct tttcatgcgc
     6841 agccgaaaac gccttcccgc cggattgcag cgcctgcgtc agaatgcgac tccccaaact
     6901 gaggtgcgtg ccgttgttgg ccaggctgat attcgaggtg gacggcagca cgacgaggtt
     6961 gcgcgtgatg atgcccgcat ccttcaaccg gacgacggcg ttcagttgac tgcgcgacag
     7021 cacctcatga cagagcccca tgtactgaga cttctcttcg cctcgctccc atcccgacaa
     7081 gcacgggttc atgaacaatt cgcggtagaa ggcatcggac accagtccgt tgaggcggcg
     7141 ctgccgcaac ggcggatggg gtgacgcata gaccaacacc tgttgtccgc tctcctgcaa
     7201 accgaattgc cgattggcat acaacgccag cagatgcgtg aacagaaacc ggaccgccgt
     7261 ttcccgcgcg atgccggtcc ccatcccctg cgcccgatcc atcggagaca cgtagaagga
     7321 gaaggtttcg ggcgaactgt tatcgttcag gaagtgattc agcaggcgtc ggccctcgat
     7381 cgcgagcagc ggcgcatccc gctcgaactc ttcgatcact tgggccagcg tgagcttcaa
     7441 caggtaactg atcgggagac ggacccactc ctcgccctgc tccgtcagca gataccgatg
     7501 ggcatcggcc cgaagcggcc ccttggcatg acgcttgtcg gctgcgagat cgccctcgaa
     7561 gagttgatcg gcggttcgat tcaggagccg ccggggaaaa cgcacccacg aattttccca
     7621 gacggtcgca tccggcgaag cgaggaactc ctgcagctgc ctgatgacga ttctcggact
     7681 ctcgccggcc gccgtccgtt ctcgaatatt gtgcagataa ttggaactct gaatcgtgcg
     7741 cgccagatcg acatcttcgg agatgccgca gaccgccgcc tgcaactcac tctcggaacc
     7801 agccgtgatg tcatcgtggg agaacggcaa aacggggaga ccggtaagga attgcgccgc
     7861 ctccttttcg agcacatcac ggggctcggc cgggcgcaga gggaaattcg aggagatggt
     7921 cgtcgttcgg ctcatgctca acctcccgat gatcacccgg agaaatctac ggctgatacg
     7981 gatcgagcct accgccggtc tgttacgagc gcctcatccc catagaaatg ttatgtaaca
     8041 ttacagcagc ggtgaggccc ttcgttagcc cggactactc atgaatgccg tcgcgaggcg
     8101 tgcgataccg aagcccgccg ggcgagagga tcggacgacg cagcaggcat tcatgaatag
     8161 tccgggggag attcagggag agagatgagc gagtacctgg cttaagggca atgcgtgaag
     8221 cgacgggacg acaagatcgg cctgcaccag acgactctct ggcaaggtcg tcgccactcc
     8281 gacgcagcgc atcccggcct tgcgggccgc ttcgatcccg tgcggcgtat cctcgaacgc
     8341 cacgcattcg tgggctctta gcggcgaccg ccgattcagc gcgccgagcg catgcagata
     8401 cagagccgga tcaggtttgc cctgaccaac atcctgcgcc gccgagacat gctcgaacat
     8461 cgacgtcatc ccggccagtt ccaaacacaa ggcaatttcc tggcgcaacg cgccggatgc
     8521 aatggccgta cggcaggtct ccgtcacggc tgcgacaaat tccctgacgc cggacagaac
     8581 aggtaacgtc ttgcgtagcg cctcctgcat gagaccggtt ttgcgttgca cgagtcggtc
     8641 gagagtctcg gccggtaccg cggtccaacc ggagtcggtc aagaccgcgc ggaagcaggc
     8701 ctgatccgtc aacccgacat agcgcgcatg gtatcggtcc ggagcaagcg tcaccccttc
     8761 cattcgaagc acctggcgga acatctcgaa atgcagcggc tcggtgtcga acaacacccc
     8821 gtcgaaatcg aacatgactg ctttgatcgt ggccatgtgt ttccacccat acctggttac
     8881 ggctaatcga cacaaccggc ctgcgcatgg ttcataagaa cttaatcatc tccttacctg
     8941 gttgtaatcc tgtgccgata cgatggtccg actgatacct ccggcctggc tcctgacagc
     9001 cgccgcgtgg ccggcctcgc agcccgcttg cagtctgcgc acacatcgcc catcatcata
     9061 ggaatcctcc gggagaatcc ccatggcacc tctcgatgcg cctccgctct cgatcgaaca
     9121 ggggacctgt tatgccctgt tcgcctatga cattggattg gcgatcaatc tcgatgaggc
     9181 ggaacggcac gtcaccgcaa tcaaagaacg cgggcgcatc cgccacaaag cgcgagcccc
     9241 gcactatttt gaataccgtc cggcaccgct tcgactgaca caggagaccg tcgctctggc
     9301 tttcggcgac taccggtcga gcgaagccgt ggaggtgatg ctgtacgact tcggcgcggt
     9361 caccgtcatc taccgcattc cgatcaacgg tccctttgac ggcctgctga gcttaagtga
     9421 gtcgctgtac gagaacgaga cgctgctgac ggaatcgcgc caccgcctgg atcaactggt
     9481 gcgcgacatc tttccggcca tcgagcggcc gtccacgtcg aaggaggtcg aggactattt
     9541 gattttttcc attgagcact gcacaccctc ggccatccaa accttcggcg ctccctacga
     9601 tccgttattc gcccacgtgc ttcgtagcga gcgcacgcga ctctccgacc aggaaatcca
     9661 tgaagcgacg tcctgccgga tttcattcag ccgcgatgac accgccctga tcgactggaa
     9721 tgccgccctc gtctttggac aggacatgga tgacgtgcgg gcggtgctgg agtttgtgaa
     9781 cgtcgaactg cttgaaatgc gcgccctgga tgagcaactc gatacggcgc tcgaccaggg
     9841 gtatgacgcg ctgacgcgaa agaccggcct gacatcatgg ctgcccggtt cgcacgaagt
     9901 cgcattgacg cacatcgggc aattgcaggt cgacagcgcc gtgctcttcg aacgcgtggc
     9961 caacacgctg aagctgctcg gcgatcaata cctggcccgc gtctatcgat tggcctccca
    10021 acgctttcac ctggaagcct gggacgccag catcatcaga aaactccaga cactcgaaag
    10081 catttacgga aaaatgaccg accgcgccac gactcgacgg atggaagtgc tcgaatggat
    10141 catcatcgtg ctcatcgcaa tttcgattgc catctccttc ctcccgttca ccagccacta
    10201 agcgcagggg ctcaggccat agcaggatgc ggagacagtc cgccagcggc attctcgcat
    10261 cgcgtagcaa tcgagttttt ccgcagcccg ctaggctcca gtgaagagat atggtagaac
    10321 ccctgctgat gaacgaccaa cgccgatctg atccagtgcg cggcacgatt cacgccgggt
    10381 ggctccgcag cctggtcggc ctgctggccg ttctcagcct cgcaggctgc ctttcccctc
    10441 cgaccctcaa tcgagccgtc ctcgcctacg acgaggccat caccgacgcg atctccaaac
    10501 agctcctcat caatatcgcg cgggcacacc atcatgagcc gattcacttc accggcgtcg
    10561 ccaacgtggc ggccaccttc gatttccgta tcagcgcggg cgccacaccg gccctcaccg
    10621 gagaacacgg ccggacattg gtttccttat tcggaggatc aatcgccgaa aatcccacca
    10681 tcagcattac gccgatcgag ggagaagaat tcactaagcg catcctggcg ccgttccagg
    10741 aaagcaaact caccctgctg ctccggcaag gagtcgacat cgatctcctc ctgcggctca
    10801 tggccaaaga actccgtctg aaacacaagg gcgaagaagt cgcctaccgc aacagtccct
    10861 ccgacaaaga cggctatgac atgttccgca aagtcgtcct gcacttgtcg gccattcaag
    10921 atgccaatca cctctatgcg gaagccttga cgttcgagcg cacctggacc atcccggccg
    10981 agtccgtgac cgccgaaggc tttgctacgc tggagcagca atacctcatc acgtaccaga
    11041 gcgagacacg cacctacacc ctgcgcaagc cggtctccgg acgcattctc attacgaatt
    11101 atgatcccgc cacgctaccg cccgcagaac gggtccgatt gcacgaaacc gcagaccagc
    11161 gccccgtcaa cgatgtctcg ttcgacattc gagcgggcca tttcggagga gagtggccgt
    11221 tgcagggcga tttcaggctg cgcagcttta acgcgatgtt gaattttttg gggcacgccg
    11281 cggacgaaga ccgcgagtat gccgtggaaa aggatgcgcg tacgcccccc gtggccgaaa
    11341 atcctgtgca cacgatggac ctgctcatcc tcgaccacac accggacgaa ccagacctgg
    11401 ccgtgaaatc ccatggccgc tattatgcga tcaatgccac gggaccccag gcgcgatgga
    11461 accgcgaagc gttcaaattg ctctcccaac tgtttcagat gactgtcacc gacgtgccgc
    11521 gaaccggcgt gccgagcatc accatcgcca aataggcctc agtcggcgga tcacccgtgc
    11581 gcggtttttt tgttcccgct ctcttgcaac tccgatggcg ctccagtagg gttggaccat
    11641 cgacagaacg cgggccgccc gactctcgcc acgagtgatg tacccgtccg cccctgattc
    11701 acacccatgc accgcaatgt catcagtcga tccctgctgt ggcagtggac agcattcttc
    11761 atcagcttgt gtctcgccct gccttcctgg gccgacgaag tacacctgca gaatggagat
    11821 cgactgaccg gcacgattct caaaatggaa gagcgcgtcc tcaccctgca gaccgactac
    11881 gggggtgagg tgaaaatcga ttggggcaaa gtcgagagcc tgaagtccga cggcacactg
    11941 aagattctgg tgccgggcga atcgcatgat gcacttcgcg attttctgta cggcgcacag
    12001 gccctgcgtg agaccagaga actgggtccc gagagtcccg tgccattggc cgatattacc
    12061 gccattaatc tcgaaccgct ccgactcacg ggaaccgtca cagtcggcgg caacagcacc
    12121 tcaggcaaca gcagcacgaa agccttcaac agcgccgccc gcttgacaat tcaagcgtac
    12181 cgccagagac tgttactcga agggaaatac aactacgggc aggccggcga gctcgtgacg
    12241 gccagaaatt cactggccag cgtgaagcac aactacttcc tcagcaagca gatcttcatc
    12301 gaatccttcg gcatgcttga aaaggacacc ttgcaaaacc tccaactccg atccacgatc
    12361 ggtagcggct tgggatatca gttttacgag agtgcccgaa ccaccctctc gctctccctc
    12421 ggtcttgccc atgtgagcga gcacttcacc aacagtccga acacgcaaac cccatcaggc
    12481 cgatggagtc tgcgctggga acatgccgta tggccggacc gcgtcaaagt cttccatcgc
    12541 catgaaggct tctacgacgt caatgccggc aatgcctttc gcatcaatgc ggaccaggga
    12601 gtccggatca ccgtctacaa gaatctgttc ttcaatgtgg aatacgacct tcgattgaat
    12661 acgcaacccg ccccgggacg ggagaaaatc gacgaatccc tcatttttgg cgtgggctac
    12721 gaattcaagt gacggccgag gcaataccta gcggcgttcc tatttgcccg cggcccgctc
    12781 gccctgttcg ccgttccctc cagcctcgct gcttccagcg atgggagtca cggctgccgc
    12841 tcgaccggcc agccgtccgg tcgcccaggc ccattgaaaa ttgaatccgc cgatccgccc
    12901 gtcgcaatcg agcacctccc cgaccagata caggccgggg accacctttg actccatcgt
    12961 gcggaagttg acctcttcga gcggaatccc cccggccgtc acttctgcaa agttccaccc
    13021 tcgatcgcgc acgacaggaa actgaaaccg ggtgagcgcc gacaggagac gatcacgaat
    13081 gtttcgtgac acctgggcgc aggcctgctg cgggtcgccc tccacatgac gacagagggt
    13141 ctcggcgcat cgctcggaca agacgtccgt caataacttc agcagcgacc gccgcggatt
    13201 cttcgccgtc tgatgcagaa accactcccg cgcctgctcc gtcgtgcgtc cgggcaggaa
    13261 atttccatac acctccgccg cctcaccacg ttctctggca aggcaccaga atcggctcgc
    13321 atccatcacc accggtccgc tgatcccgaa atgcgtccag agtaggctgc cgatccgccg
    13381 atcgaccggc cgcccctgca gcaccgtctg gatttcgacc tcctgtgaaa gcccggacag
    13441 cccgctatga aagcaccgat cgtcgaggac cagggccacc aacgccggga cagtcggcgt
    13501 cacccgatgg cccaggcgtc gtgcgaggcc ataacccgaa ccgtcactcc ccgtccgtgg
    13561 caacgaccgt ccgccggtgg ccaggatgac acgccgcgcg aaggtctcgc cttcttggtg
    13621 ccggatcacg aaccgggcag gctccggctt gttcgcgcct tgtgtgtcgt caaggcgggc
    13681 aatatcggtg actcgatgac cgggacgaag gacaatcccg agcgcccggc tgcgagcaag
    13741 gagcgcctcc aatacggtcc gcgccttgtc ggtcaccgga aacagtttgc cggtttcttc
    13801 acacttcaga tcgacaccga gggaagcaaa ccactggatc gtgtctcgaa cagggaaggc
    13861 cgcgagcaca ttgcgcacca ggtggcgggt accgaaaaaa tcatccggcg tcaccacatc
    13921 atgggtgaca ttgcaccggc ctccgccgga caccaggatc ttggccccga tggtctttgc
    13981 cccgtccagc aattcgattg tcaggtgagg attccgctcg gcggcaaaaa taccggccgc
    14041 cagcccggcc gctccggcgc ctacgatgca gagatcaacc gttggaggaa tcattcattc
    14101 gtctcatcta cttggcagac cgaggaccgc cgcacggagc gatcgcctaa gacaaccgct
    14161 gactgatacc ggcgagcccg ggcatggcag gccggcgggt cgaccgacgg agcggcgtat
    14221 gatacagtcg cgcgacaagt ttcagccctc tatctccctg tctcgaccta tggccggatc
    14281 gaccctgcgc atcgcacttt tacatctggc tccgataccc ggcgccctgg cgaaaaatcg
    14341 tcacctgatc gctcacgcga tcacgaaagc cgcgtgcctc ggagccgcct ggatcatcac
    14401 gccggaactc gcggtcagcg gctacacctt cgccgataca ctcggcacgg actggatcgc
    14461 cccgcagccg gatccctgga tggagaaggt ccgcctattt gccgccagga agcgcgtcgc
    14521 catcttcctc tcacatcccg agcgggaccc gcaatccggc cgactctaca acaccgtctt
    14581 cgccatcacc cccgacggac gcctggcagg atcccatcgc aagatcaatg cgctgcgcgt
    14641 cgggtccgaa gcctggtcga ctcccggcac ggaaacgacg gtctttccaa tggagccact
    14701 cgggaacgtc gggatgctca tctgcgccga cgccttctcc ccaggcattg ccaatagctt
    14761 gaaggcgaag ggcgcgcaga tcctggtctc gtcggcggcc tgggctcctg gactgcacgg
    14821 cccgaacggc gagtgggaac gatgcacgaa agacaccggt ctgcccctct tcgtgtgcaa
    14881 ccgcaccgga gccgatcgca ccctcgattt caggaaggcg gacagtgtgg tggctcagga
    14941 cgggcggcgg ctcctctcga tgtcctcgga acggtctgcc attttcctca tcgactggga
    15001 tctgaaagca g
//
LOCUS       DAORRQ010000079        14291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_214_length_14291_cov_17.540391, whole genome shotgun sequence.
ACCESSION   DAORRQ010000079 DAORRQ010000000
VERSION     DAORRQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214_length_14291_cov_17.540391"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PK224_17820"
     CDS             complement(<1..1251)
                     /locus_tag="PK224_17820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRB17659.1"
                     /translation="MNDLVPADCSPAVDRRPPHAAESDPSPGLLVRIARRIVCARLRR
                     LVQGTITVMEGPRRTVFGEPAAVSTLKAMITILEPDCYLDIAWSGTVGAGESYARGDW
                     HCDDLTALVRIFALNRSLVDGMERGVARLSAPLLKVAHRLRRNTKTGSRQNIAAHYDL
                     SNEFFAQMLDRTMMYSCAYFQRPDSTLEEASRAKLDLICRKLHLTEDDHLVEIGSGWG
                     GLACHAAKTYGCRVTTTTISRAQYELAVRRVREDGLSDRVRVLLTDYRDLPALGLRFD
                     KLVSVEMIEAVGHEYYPTFFDVCSRLLKPDGLMLLQAITIDERYYERAKRSVDFIQRF
                     IFPGSCIPSVSTLCRAMAQASDFSLLHLQDIGAHYPPTLRGWRDNLRKNVEHVGRLGF
                     RSEFLRLWDFYLSYCEGGFLERSIS"
     gene            complement(1248..2033)
                     /locus_tag="PK224_17825"
     CDS             complement(1248..2033)
                     /locus_tag="PK224_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRB17660.1"
                     /translation="MKSCVYEGTVRHRRDAPVRHSFHYSIFMMYLDLAELPDLFHERW
                     FWSPATPNLAWFRQSDHLGDPEKPLDQNVRDLVESKTGRRPAGPIRLLTHLRYFGYGF
                     NPVSFYFCFDPADRLVETVVAEVTNTPWGERHCYVLGASLDEGIGRRHRYRFGKQFHV
                     SPFMGMDVDYDWRFGTPGAALSVHMENFRGGERFFDATMVLRREEMTGRSLARVLLRY
                     PLMTTQVIAAIHWQALRLWLKRCPVIPHPDRSRHSVDARRISG"
     gene            complement(2030..3289)
                     /locus_tag="PK224_17830"
     CDS             complement(2030..3289)
                     /locus_tag="PK224_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRB17661.1"
                     /translation="MKLAIVGTGIAGMTAAHVLHRDHDLTIFEAGSHIGGHTNTVDVN
                     LQGATYAIDTGFIVFNDWTYPNFMALLDRLGVASQPSDMSFSVRCEETGLEYNGTTLN
                     SLFAQRLNLLRPSFYRMIRDILRFNRESLELLDEPGPGPSLGAYLEQNRYSPTFIRQY
                     IVPMAGAIWSAGRATMQEFPARYLVQFFKNHGMLSVDARPTWRVVTGGSRQYVECLVR
                     PFRDRIRLQSPVESVTRYADRVEVRGCDPRGIHRTESFDGVLLACHSDQALALLADPS
                     PLEKEILGAIRYQPNEAALHIDRSLLPRRRLAWAAWNYHLVPNPPDHALVTYHMNRLQ
                     GLSAPYDFCVTLNRTEAIDPATILKRITYHHPVFSPEAIAAQQRHGEINGCRRTWYCG
                     AYWGFGFHEDGVKSAMTACQALTAGHA"
     gene            complement(3286..4056)
                     /locus_tag="PK224_17835"
     CDS             complement(3286..4056)
                     /locus_tag="PK224_17835"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB17662.1"
                     /translation="MSRTSLNARRVWVTGASTGIGRAVTLELVRRGATVFASARNESA
                     LNELVRDCGGDRVTALPFDVTDRAANQRAVNDMHRRAGGLDIAVLNAGTCEYVDVAAF
                     DSALFERTIRTNFLSMVYGIEAALPLLRRSPAPQLVGMSSTVAYRGLPRSEAYGASKA
                     AIKNLFESLQVDLAPEGISVSVVCPGFVRTPLTDRNDFPMPFRIEAGDAARRIVDGIE
                     ARKPEIHFPRRFSLLFKCLTLLPSRWYIGLCARMVKQP"
     gene            complement(4060..4476)
                     /locus_tag="PK224_17840"
     CDS             complement(4060..4476)
                     /locus_tag="PK224_17840"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRB17663.1"
                     /translation="MQPVVERFKQIYSGLSVRSLGLLNELYSDDVEFQDPFHRIAGLP
                     ALRQYLAALYAQVEACSFRFEDDVRQGNCAVLTWTMQLNHPRLNAGAAVTVPGSTLIR
                     FRDKVFYHRDYFDAGAMVYEQLPLIGMLIRTIKGRV"
     gene            complement(4556..5155)
                     /locus_tag="PK224_17845"
     CDS             complement(4556..5155)
                     /locus_tag="PK224_17845"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HRB17664.1"
                     /translation="MMHEPGEISEWLVVIGLAVSVLVSSPVVGGGVEIEGVTFADTFH
                     AGPLPMPLQGVGVAKFLRTIKVYVGALYLAPGTKPERVLDDVPKRLELSYFRAIQAGD
                     FGRAAMKVLADNVSPATITRLKPRIEQLHRLYQDVKPGDRYGLTYLPGVGTELALNGE
                     RKGIVEGADFAAAYFAIWLGPDPINEALKSGLMHRREGG"
     gene            complement(5219..5728)
                     /locus_tag="PK224_17850"
     CDS             complement(5219..5728)
                     /locus_tag="PK224_17850"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRB17665.1"
                     /translation="MATVRQGVILVGHGGIPRGCPQDLVTKLKRLEGQRRAVKQPASA
                     EELELDAKIRQWPRNAETDPYQAGLEKVAAQLRANLGDVLFAVAYNEFCAPTLEESVE
                     QLVKQGATHITVATTMFTPGGSHSEVEIPEILEHLRPQYPGVELRYAWPFDLSLVAGT
                     LAEQVRRFS"
     gene            complement(5769..6137)
                     /locus_tag="PK224_17855"
     CDS             complement(5769..6137)
                     /locus_tag="PK224_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17666.1"
                     /translation="MPHVVIESTGELQDVYQAFAPMLQRAEGEILKVQECYLAKSGRE
                     ALLDAVVIEQGTARSFFIQLKRHETTITVRLLPSTDPEKTPAVKKAMALVAGLIRKVY
                     PASRYGKTNLQEYLESPMSM"
     gene            complement(6150..7103)
                     /locus_tag="PK224_17860"
     CDS             complement(6150..7103)
                     /locus_tag="PK224_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HRB17667.1"
                     /translation="MMTPPLRLGISRCLLGEEVRFDGGHKRDVFLTDVLGRYVEWVPV
                     CPEVEAGLGTPREAMRLIGNPQQPRLVAIKSGRDHTLALETMAVKRLSDLEELDLSGF
                     VFKKDSPSCGVERVRTYNEHGMPGRKGVGLFARAFLDRFPLIPVEEEGRLCDPLLREN
                     FIERVFCYRRWQDLVQRGVTRQGLVRFHTVHKYLLLAHSAPHYRQLGRLVAQVNEYRP
                     KDLAIRYGELFMQALAAKATVRKHVNVLQHILGHFKERLDAQEKAELLSVIGDYHQGL
                     TPLIVPLTLIKHYVQIFDVEYIREQVYLNPHPKELMLRNHV"
     gene            complement(7100..8545)
                     /locus_tag="PK224_17865"
     CDS             complement(7100..8545)
                     /locus_tag="PK224_17865"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRB17668.1"
                     /translation="MKGLVWFRRDLRLRDNPALSAACQECQDIVPLFVFDEPLLRSHA
                     FGSACVGFMLGCLEELRRSLALHGVSLAWRTGDPVESVIQAASDFKVDAVYWNRDYEP
                     AALERDRTVQQRLAQQGRTVRTFKDHVVFEAEEVRGQSGDPFQRYSAYRDRWWAKWRA
                     AAPPLLGIPSFPPAGKASASDSREWPSVADLGYESVPMWIEPGEQAVHARLQWFLGGP
                     VHQYVTGRNLPASDGTSKLSPHLRFGTVSARTLVHAALNALAQGGAVSRADVFTWIDE
                     LVWREFFQQVLTAFPHVAQGPFKVKPGLPEPRPAGPERDRLFAAWSQGLTGYPIVDAG
                     MRQLNQTGWMHNRVRMVVASFLVKDLRIDWQSGERYFMERLVDGDLAANNGNWQWCAS
                     TGTDAMQGYRIFNPRIQSEKFDAEGEYLRRYVPELKHVPTKWIHEPHLMPPLEQVQAG
                     CRIGSDYPEPIVDHRQARQEYLDLGKAQVKA"
     gene            complement(8542..8973)
                     /locus_tag="PK224_17870"
     CDS             complement(8542..8973)
                     /locus_tag="PK224_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17669.1"
                     /translation="MTGHTTSRGIDIKLFGALFLLVGLIDVLIIEFFPAYGLKLFGVT
                     ITGPLAYMVKLHSPAVHFLIGYGFLFLRPWAWGLAMAYGGFGLTSELMNQFQFGFHHL
                     RTGFMATTALFLCYVAWRRILFADPVPSAQRLASSSPEVPL"
     gene            complement(9022..9546)
                     /locus_tag="PK224_17875"
     CDS             complement(9022..9546)
                     /locus_tag="PK224_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17670.1"
                     /translation="MNRMLAAIGLSIIVAGQLTVEAQGQMAPVSRTSSVERTGLFLMA
                     GDYRRALEACEQAIQERPSAEAYLQLTYVYQAIDAYLDQLSKDESWMAVEQLYLNLAY
                     RHTEDLVDPPGGLARMAKEMIQTSVRQQSDVSAAMAVRLNKATADRLWQEQAQWRHTH
                     PTEWWRAFPDSWVR"
     gene            complement(9554..10036)
                     /locus_tag="PK224_17880"
     CDS             complement(9554..10036)
                     /locus_tag="PK224_17880"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRB17671.1"
                     /translation="MKRHINTSAYLVVGFLSICLSYSSAATGTAWASDGHEKAGAPAP
                     TFSLEATIPDGVIGVSLHVGAEHVGDTAVLYVAHVLPDGPAEKAGLKQGDELTTVDGV
                     AVTGKTYEQVALMVRGEPGSVVKLGVKSEGGSREVSVTRVSGQTLYKGQMGSHGGSGR
                     "
     gene            complement(10059..10541)
                     /locus_tag="PK224_17885"
     CDS             complement(10059..10541)
                     /locus_tag="PK224_17885"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HRB17672.1"
                     /translation="MSALLDSVMQYLQALAVAVLVGKVVLLSFVVAPILARNLDQDAF
                     GKVVRRLFPAYYVLGMGAATAGLLSVTVLGSTRGWNLPVLIAGGIWLVILAAESYCRS
                     PLTPQSNAMRDRLKEQEQQGAVDAPLKRAWDRLHQRSLYLNSLVLLAGLCLVGLARQL
                     "
     gene            complement(10517..11455)
                     /locus_tag="PK224_17890"
     CDS             complement(10517..11455)
                     /locus_tag="PK224_17890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRB17673.1"
                     /translation="MKIIVAGGTGFIGHALVEELARQGHEVVVLSRRSGHASQSSVRY
                     VEWDACSAGPWHSEVATADVVINLAGAPIADGRWTDARKRLLVESRILSTRLLVDAVA
                     GRSASLPLLISASGIGYYGPSDDRLLDEASSIGQGFLAELSAAWEAEALRAGQLGTRV
                     VLLRTGMVLEQGGGALPKMLLPFRLFAGGPVLPGTQWVSWIHRSDLIGLIQWVIATAT
                     ISGPVNAVAPEAVTMKTFCATVGRVLHRPSWLPVPGLALHLALGELGTLMTTGQRVDP
                     AKARAGGYTFRYPTLEPALQAIINKEDQACLPSSTR"
     gene            complement(11488..11766)
                     /locus_tag="PK224_17895"
     CDS             complement(11488..11766)
                     /locus_tag="PK224_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17674.1"
                     /translation="MTQHYIDLLDDTGQIDIMAWHCTMCGEVLDPVILKNRRSPPPNL
                     LYGTKQRKFSQRVTAVTGSRPVKQSSDDRAPDESDEESADEADQDGAS"
     gene            complement(11939..12070)
                     /locus_tag="PK224_17900"
     CDS             complement(11939..12070)
                     /locus_tag="PK224_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17675.1"
                     /translation="MTQMIAYGIAAMVVSIGAALFVRQRRAAYVVVRPARPERSRVQ"
     gene            complement(12260..13048)
                     /locus_tag="PK224_17905"
     CDS             complement(12260..13048)
                     /locus_tag="PK224_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17676.1"
                     /translation="MSLFVTAACAVALTPVLAAPTLKELDPVPMVTVPGGPFLMGSEN
                     PKGRADEWPQRSVHVDTFAIDQVEVTNERYMTFVATTGHRSPPNPYGTGVLVSAKGIE
                     QLPVVQVTWYDAKAYCAWAKKRLPTEAEWEKAARGTDGRMFPWGNETPSPTRANFDRE
                     WVDDKTLHAVGSLSGGESPYGIKDMSGNAREWVQDWYAPDYYANAPDKNPQGPDKGIV
                     RVIRGGSWHSPQSDITATARGRGGFALQTHGTGFRCVRGGETAK"
     gene            complement(13087..13455)
                     /locus_tag="PK224_17910"
     CDS             complement(13087..13455)
                     /locus_tag="PK224_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17677.1"
                     /translation="MRYSTMITLVGGFTALLMTGDLQAGPIDASRHPHPEKLQMVHEA
                     EHSVDHAWEVYHRAALGGTVASPDLQAQIEQHLHEARTLVTQAQEAADRGDTGKVERL
                     VGEIKIHTAQAIAGSKEQKK"
     gene            complement(13508..>14291)
                     /locus_tag="PK224_17915"
     CDS             complement(13508..>14291)
                     /locus_tag="PK224_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17678.1"
                     /translation="YEVSNKDYGAFIKATGHPAPAYWDDPRLNKPQQPVVGVNWIDAK
                     TYCEYRGKRLPTEAEWEKAARGPHGNLYPWGNDFDSNKANYDKHHDATLPVDSHPEGA
                     SYYGVHNMAGNVFEWVGDWYDPKYYGRLETMVNPTGPEKPLWIGGSGTYVDRLTVGEK
                     RVIRGGSWIAPEGTIRSTHRFWNHPLNNSYGVGLGFRCAKTAPADWEQRIRDSYITAL
                     VEMGRERFSEAQQAVNRGLAMDPVNVELLELRPLIEQSMKQR"
BASE COUNT         2860 a         4760 c         4021 g         2650 t
ORIGIN      
        1 cgaaatcgac cgttcgagga atccgccttc gcagtaggat aaataaaaat cccacagccg
       61 caggaactcg gaacgaaatc cgagccgccc cacgtgttcg acattcttcc gcaagttatc
      121 gcgccaccct cgcaaggtcg gcgggtaatg cgcgccgata tcctgcagat gcaacaggct
      181 gaagtccgag gcctgcgcca tcgcccggca caaggtactc accgagggga tacaactacc
      241 ggggaagatg aagcgttgga taaaatcgac cgaccgcttc gcccgctcat agtaccgctc
      301 atcgatggtg atcgcctgga gcagcatcag cccgtcgggc tttaacaggc gactgcagac
      361 atcgaagaac gtggggtagt attcatggcc cacggcctcg atcatctcca ccgaaaccag
      421 cttgtcgaac cgcaagccga gcgcgggcaa atcccgataa tcggtgagca gcacccgcac
      481 acgatcggac aggccgtcct cacgcacccg gcgaaccgcc agttcgtact gcgctcggga
      541 aatcgtggtc gtcgtcactc gacaaccgta ggtcttcgcg gcgtggcagg ccaggccgcc
      601 ccagccggat ccgatttcca ccagatggtc gtcttccgtc agatggagct tgcggcagat
      661 caaatcgagc ttggcgcgcg aagcctcctc cagcgtgctg tcgggacgtt ggaaataggc
      721 gcaggagtac atcatggtcc gatccagcat ctgcgcaaag aattcgttgc tcaggtcgta
      781 atgggccgca atgttttgcc ggcttcccgt tttggtattc cgccgcagcc ggtgcgcaac
      841 cttgagcaac ggtgcggaca agcgcgccac cccccgctcc ataccgtcca cgagcgatcg
      901 attcaacgcg aaaatccgga ccagcgccgt gaggtcgtcg caatgccaat cgccacgggc
      961 ataactttct cccgcaccca ccgtcccgct ccacgcgatg tcgagataac agtcgggctc
     1021 gagaatagta atcatcgcct tgagcgttga aacggcggca ggctcaccga agaccgtgcg
     1081 gcgcggcccc tccatcaccg tgatcgttcc ctggacgagc cggcgcagac gggcacagac
     1141 gatgcgtctg gcaatccgga ccagcaaacc gggagaagga tcagactcag cggcatgcgg
     1201 tggccgccgg tctacggcgg gtgaacagtc ggccggcacg aggtcattca tccggatatc
     1261 ctccttgcat cgacggaatg cctggacctg tcgggatggg gaatgacggg acaacgtttc
     1321 aaccacaggc gcaacgcctg ccaatggatc gccgcaatca cctgcgtcgt catcagcggg
     1381 taccgcagca acacacgggc cagggaccta ccggtcatct cctcccggcg caggaccatc
     1441 gtggcatcga agaaccgttc tccaccgcgg aaattctcca tgtgcacgga aagagcggca
     1501 ccgggcgtgc cgaaccgcca atcgtagtcc acatccatgc ccatgaacgg agagacgtga
     1561 aattgcttgc cgaagcgata ccggtgtcga cgaccgatcc cctcatccag ggaagcaccg
     1621 agcacataac aatgccgttc gccccacggt gtgttcgtca cttcagccac aacggtctca
     1681 accagccgat cggcggggtc gaaacaaaaa taaaagctca ccgggttgaa gccgtacccg
     1741 aagtaccgca ggtgcgtgag cagccggatc ggaccggccg gccggcgacc ggtcttcgat
     1801 tcgacgagat cacgcacatt ctgatccaga ggcttctccg gatcgcccag atggtcgctc
     1861 tgcctgaacc aggccaggtt gggggtcgcg ggagaccaga accagcgttc atggaagagg
     1921 tccggtaatt cggccagatc cagatacatc atgaagatcg agtagtggaa cgaatggcgg
     1981 accggcgcat cgcgtcgatg cctgaccgtt ccttcataca cacagctctt catgcatgcc
     2041 ccgccgtgag cgcctgacag gccgtcatgg cgctcttcac gccgtcttca tggaaaccga
     2101 agccccagta ggccccgcaa taccacgtcc ggcgacatcc gttgatctcc ccgtgccgtt
     2161 gctgcgcggc gatcgcttcg ggggagaaca ccggatggtg gtaggtgatg cgcttgagga
     2221 tcgtcgccgg atcgatcgcc tcggtgcgat tcaacgtcac acagaaatcg tacggcgcgc
     2281 tcagcccctg aagccgattc atgtgatagg tcaccaaggc atggtccggc gggttcggca
     2341 ccagatgata gttccaggcc gcccaggcca agcggcgtct cggtaggagc gatcgatcta
     2401 tgtgcaacgc cgcttcgttc ggttgataac ggatcgcccc gagaatctcc ttctccaggg
     2461 gagacgggtc cgctaacagc gccaacgcct ggtcgctatg gcaggcgaga aggactccgt
     2521 cgaatgactc ggtccggtgg atcccgcgcg ggtcgcatcc ccgcacctcg acacggtccg
     2581 cgtaacgagt gaccgattcc accggagact gaagccggat gcggtcacgg aagggccgca
     2641 ccaggcactc gacatattgc cgggagccgc cggtcaccac cctccaggtc ggacgcgcat
     2701 cgaccgacaa catgccgtgg ttcttgaaaa actgaacgag gtaccgcgcc ggaaactcct
     2761 gcattgtggc gcggccggcc gaccagatgg cccccgccat gggaacgatg tactgacgaa
     2821 taaacgtcgg cgagtaacgg ttctgctcca gatacgcgcc cagcgaaggg ccggggcccg
     2881 gttcatccag caattccagc gactcccgat tgaagcggag aatgtcccgg atcatgcgat
     2941 agaacgaggg ccgcagcaga ttgagccgct gggcgaaaag gctgttcaac gtagtgccgt
     3001 tgtactccaa cccggtctcc tcacaccgca cactgaagct catgtcgctc ggctgcgagg
     3061 ccacacccag gcgatccagc agggccataa aattcgggta ggtccagtcg ttgaatacga
     3121 taaacccggt atcgattgca taggtcgccc cctgcagatt cacatcgacc gtgttggtat
     3181 ggccgccaat gtgtgagccg gcctcgaaga tggtgaggtc atggtcgcgg tgcagcacat
     3241 gtgccgccgt cattcccgca atgcccgttc ctacaatggc aagcttcatg gctgcttcac
     3301 catcctggcg cagaggccga tgtaccaccg gctcgggagc agggtcaggc acttgaagag
     3361 caggctgaat cgcctcggga aatgaatctc cggcttccgg gcctcgatcc cgtcgacgat
     3421 gcgtcgggcc gcgtcaccgg cttcgatcct gaacggcatc gggaaatcgt tccggtcggt
     3481 caacggtgtc cgtacgaacc ccggacacac gaccgacacg ctgatacctt cgggcgcgag
     3541 atcgacttgc agcgattcga agaggttctt gatcgccgcc ttcgacgccc cgtaggcttc
     3601 ggagcgaggc aacccgcgat aggccaccgt actgctcatc cccacgagct gcggcgccgg
     3661 tgaccgccgc aacagcggga gcgccgcttc aatcccatag accatgctca ggaagttggt
     3721 gcggatcgtc cgctcgaaca gcgcactgtc gaatgccgcc acatccacgt actcacaggt
     3781 gccggcgttg agaacggcaa tgtccaggcc ccctgcacgg cgatgcatgt cgttcaccgc
     3841 gcgctgattg gcggcccggt cggtcacatc gaagggaagc gccgtgacac gatcgcctcc
     3901 gcagtcacgg acgagctcgt tgagcgccga ttcgttccgc gccgaggcaa agacggtggc
     3961 cccgcgacga acgagttcca gcgtgaccgc ccggccgata ccggtcgagg cgccggtcac
     4021 ccacacccgt cgagcattga gactggttcg gctcatgggt cacactcggc ctttgatcgt
     4081 ccggatgagc atgccgatca gcgggagctg ctcgtacacc atcgcaccgg catcgaagta
     4141 gtcgcggtgg tagaacacct tgtcccgaaa acgaatgagc gttgaaccgg gaaccgtcac
     4201 cgccgcgccg gcattgagcc tcgggtgatt cagctgcatg gtccaggtca atacggcaca
     4261 attgccttgc cggacatcgt cctcgaaccg gaatgaacag gcctccacct gcgcgtagag
     4321 cgcagccaaa tattggcgca gggcggggag tccggcgatc cggtgaaagg ggtcttgaaa
     4381 ctcgacgtcg tcgctgtaca actcgttcag cagtcccaac gagcgaaccg acagtccgct
     4441 gtagatctgt ttgaatcgtt cgacaaccgg ttgcacgaca gcggactcct ctcctgctct
     4501 tctacgaagg aacctgaggg acggattctt tcagcgcccc ccatcatcac cggcgctagc
     4561 ccccctcccg ccggtgcatg agccccgatt tcaacgcttc attgatcgga tccgggccga
     4621 gccagatggc gaagtaggcg gcggcaaagt cggccccctc tacaatgccc ttccgttcgc
     4681 cgttgagggc aagttcggtc ccgacaccgg gaagataggt cagcccgtaa cgatctcccg
     4741 gcttcacgtc ctgatagagc cgatgcagct gctcgatacg aggcttcagg cgcgtgatcg
     4801 tggccggcga cacattgtcg gccagcacct tcattgccgc ccgcccgaag tccccggcct
     4861 ggatggctct gaagtaagac aactccagac gcttcggcac atcatccaac acgcgctcag
     4921 gtttcgtccc cggcgcgaga tacagtgccc cgacatacac cttgatcgtg cgcagaaact
     4981 tcgctacccc caccccctga agcggcatcg gcaggggacc ggcgtggaac gtatcggcaa
     5041 acgtcacgcc ttcgatttca acccctccgc cgacaacggg cgaggacacg agtaccgaaa
     5101 ccgccagccc gatgacgaca agccactctg atatttcccc tggttcgtgc atcatccgac
     5161 tcccaggtca cccctgaccg gcatcccggc cgcacctctc cgtaccccgg aactccggtc
     5221 aggagaatcg ccgcacctgc tcggccagcg tcccggcaac cagactcaaa tcaaacggcc
     5281 aggcatagcg caactcgacc ccgggatact gcggtcgaag atgctcgagg atttcaggaa
     5341 tctcgacttc cgaatgcgaa ccgcccggcg tgaacatcgt cgtcgccacc gtaatgtgag
     5401 ttgccccctg cttgacgagt tgctcgaccg actcttccag tgtgggtgcg caaaactcgt
     5461 tataggccac ggcaaacaac acatcgccca ggttcgcgcg caattgcgcc gccacctttt
     5521 caagcccggc ctgatacgga tccgtttcgg cgttcctggg ccactggcgg attttggcat
     5581 ccaattccag ttcttcggcc gaagccggtt gtttcaccgc gcgccgttgc ccctccagcc
     5641 gtttcaattt cgtcaccaaa tcctgcgggc atcctctggg gataccgcca tgcccgacga
     5701 gaatcacacc ctgtcgcact gttgccatgt catctctcct ctgattgaaa attcacacct
     5761 ggttacactc acatcgacat cggggactcc agatactcct ggagattggt cttgccgtag
     5821 cggctcgccg ggtacacctt ccggatgagt ccggccacca gcgccatcgc ctttttgacg
     5881 gccggcgtct tctcgggatc ggtggaggga agaaggcgga cggtgatggt cgtctcgtgc
     5941 cgtttcagct gaatgaaaaa actgcgcgcc gtaccctgct cgatcacgac cgcatccaag
     6001 agcgcttctc gtccgctctt cgccaggtag cactcctgaa ccttcaaaat ctcaccctcc
     6061 gcccgctgca acatcggagc aaaggcttgg tacacatcct ggagttcacc ggtcgattcg
     6121 atcactacgt gcggcatagc cgctcctttc tagacatgat tgcggagcat caattccttc
     6181 ggatgcggat tcagatacac ctgttctcga atgtattcga catcgaagat ctgtacgtaa
     6241 tgtttgatca atgtcagcgg aacaatcagg ggcgtgagtc cctggtgata gtccccgatc
     6301 acgctcaaca gctcggcctt ttcctgcgca tccagccgtt ccttgaaatg gcccaggatg
     6361 tgctggagca cgttcacatg ttttcgcacg gtcgccttcg ccgcgagggc ctgcataaag
     6421 agttcgccgt accggatggc caggtctttc gggcgatact cgttcacctg cgcaaccagg
     6481 cgcccaagct ggcgatagtg cggggcgcta tgggccagga gcagatattt gtggaccgta
     6541 tgaaaccgca ccaatccctg cctcgtgacg ccgcgctgca ccagatcctg ccaacgacgg
     6601 tagcagaaca cccgttcaat gaagttttcc cgcagcagcg gatcgcagag ccgcccttct
     6661 tcctccaccg gaatcagcgg aaaccgatcg agaaaggccc gcgcaaacaa tcccacaccc
     6721 tttctgcccg gcatcccgtg ttcgttgtac gtgcgcaccc gctccacccc gcaactcggc
     6781 gagtctttct tgaacacaaa cccggacagg tccaactcct caagatcact caggcgcttc
     6841 acggccatcg tctccaacgc cagcgtgtgg tctcgcccgc tcttgatggc gaccagccgc
     6901 ggctgttggg gattgccgat gagtcgcatc gcttcacgcg gcgtgccgag cccggcttcg
     6961 acttccggac agaccggcac ccactccacg taacgcccca gaacatcggt cagaaacacg
     7021 tcccgcttgt ggcctccatc gaaacggacc tcctcgccaa gcagacaccg gctgatgccc
     7081 aggcgaagcg ggggggtcat catgccttca cctgcgcctt tccgagatcc agatattcct
     7141 ggcgcgcttg tcgatggtcc acgatcggct ccggataatc cgacccgatg cggcatccgg
     7201 cctgcacctg ttccaacggc ggcatgagat gcggctcatg aatccatttc gtcggcacat
     7261 gcttgagttc cgggacgtag cggcgaagat attccccctc ggcatcgaac ttttcgcttt
     7321 ggattctcgg attaaaaatc ctgtagccct gcatggcatc cgtccccgtc gaggcgcacc
     7381 attgccagtt gccgttgttg gcggccaggt ctccgtcgac gagccgttcc atgaaatagc
     7441 gctctccgct ctgccaatcg atccggaggt ccttcaccag aaacgaggcg accaccattc
     7501 gcactcgatt gtgcatccat cccgtctggt tcaattgtcg catgccggca tccacaatcg
     7561 ggtagcccgt gaggccctga gaccaggcgg caaacaaccg atcgcgctca ggccccgcgg
     7621 gtctgggctc gggaagtccc ggtttcactt tgaacggccc ctgcgccacg tggggaaacg
     7681 ccgtgaggac ttgctgaaaa aattcccgcc agaccagttc atcgatccag gtgaagacgt
     7741 ccgcgcgcga caccgcgccg ccttgtgcta gcgcgttcag cgccgcatgc accaacgtgc
     7801 gtgcggagac cgtcccgaaa cgcagatggg gagacaactt ggaagtaccg tcgctcgccg
     7861 gaagatttct gccggtcaca tactgatgga ccgggcctcc caggaaccac tgcagtctgg
     7921 catgcacagc ctgttcaccc ggctcgatcc acatcggcac cgactcatat ccgagatccg
     7981 ccacggaagg ccattcccgc gagtcagacg cggacgcctt gccggccggc ggaaacgagg
     8041 gaatgcccag cagaggaggg gcagcggccc gccatttcgc ccaccagcgg tcgcgatacg
     8101 cgctgtagcg ctgaaacggg tctccgctct gcccgcgcac ttcctctgct tcgaacacga
     8161 catggtcttt gaacgtcctg accgtgcgac cctgttgtgc cagccgctgc tgaaccgtgc
     8221 gatcccgctc cagcgccgcc ggttcatagt cccggttcca atacacggca tcgaccttga
     8281 agtcggacgc cgcctgtatg accgattcaa ccggatcgcc cgtccgccag gcgagggaca
     8341 cgccgtgcag ggccagggac cggcgcaact cctccagaca ccccagcatg aacccgacgc
     8401 aggcggaacc gaaggcgtgc gaacgcagca gcggttcatc gaacacgaac agcggcacaa
     8461 tatcctgaca ttcctgacag gccgccgaca gggcggggtt gtcacggagc cggagatctc
     8521 gacgaaacca gaccagacct ttcataaggg aacctcggga gaggaggagg ccagccgctg
     8581 cgccgaaggg acaggatcgg cgaacaggat tcgccgccag gcgacataac agagaaacaa
     8641 ggccgtggtg gccatgaagc ccgtgcgcaa gtgatgaaag ccgaattgaa actgattcat
     8701 caattcgctg gtgaggccga agcccccata ggccatggcc aacccccagg cccacggacg
     8761 cagaaacaga aacccgtagc cgatcagaaa atgcacggcc ggcgaatgca gcttgaccat
     8821 gtaggccagc ggaccggtga tcgtgacgcc gaacagtttg agcccatagg cggggaaaaa
     8881 ttcgatgatc agcacatcga tcaatcccac cagcagaaag agcgccccga agagtttgat
     8941 gtcgatgccc cgtgaagtcg tgtgcccggt catgtgttca cgctccttcc ctgcggacgg
     9001 actccgtggg tccgtgctca ttcatcgaac ccaggaatcc ggaaacgccc gccaccattc
     9061 ggtgggatgt gtgtgccgcc actgtgcctg ctcctgccag agccggtcgg ccgtagcctt
     9121 gttcaaccgc accgccatcg cggcgctgac atcggattgc tggcgcacgc tggtttgaat
     9181 catttccttc gccatgcgag ccaggccgcc cggcggatcg acgagatcct ccgtgtgtcg
     9241 ataggcaagg ttcagataca gttgttcgac ggccatccag ctctcgtcct tggagagctg
     9301 gtccaggtag gcatcgatcg cctggtagac ataggtgagc tgaaggtagg cttctgccga
     9361 cggccgctcc tgtatggcct gctcacaggc ctccagcgcc cggcgatagt cgcccgccat
     9421 caggaataac cccgttctct cgacgctcga ggtccgcgag acaggcgcca tctgtccctg
     9481 cgcctcgact gtgagctgtc cggccacgat gatcgacaat ccgatcgccg caagcatccg
     9541 gttcatatgc cacctatcgg cccgagccgc cgtgtgatcc catttgtcct ttgtacagag
     9601 tctggcccga tacacgggtc acggagacct cgcgcgaacc gccctcgctt tttaccccca
     9661 acttcaccac cgacccgggc tcgcctcgaa ccatgagggc cacttgctcg taggtcttcc
     9721 cggtcacggc aacaccgtct acggtcgtca gctcatcccc ttgcttgagc ccggctttct
     9781 ccgccggtcc atcgggcaat acgtgcgcga catagagcac ggccgtgtcg cccacgtgct
     9841 ccgccccgac gtgcagcgac actccgatca cgccatccgg aatcgtggct tccaaactga
     9901 atgttggtgc cggcgcccct gccttctcat gcccgtcgct tgcccaggct gtgccggttg
     9961 cggcagatga gtaactcagg cagatgctca agaaccctac gaccaagtat gcggaagtat
    10021 tgatgtgcct tttcatcgtc catctccctt cgtgacaatc acaattgacg cgcgaggccc
    10081 actaaacata gtcctgccag caacaccaac gagttcagat acaacgaccg ttgatgtaat
    10141 cgatcccagg cccgcttcaa cggcgcatcg acggcaccct gctgttcctg ttccttcagt
    10201 cgatcacgca tcgcattact ctgcggagtg agaggcgacc ggcaatagga ttctgccgcg
    10261 agaatcacca gccaaatacc gcctgcgatc agcaccggca gattccagcc acgtgtgctc
    10321 ccgagcacgg tgaccgacaa cagccccgcc gtagcggctc ccataccaag cacatagtag
    10381 gcaggaaaga gccggcgaac gacttttccg aaagcatcct ggtccaggtt cctggccaga
    10441 atcggcgcca ccacaaacga cagcagcacg accttgccga ccaagaccgc aacagccagt
    10501 gcttgcaggt actgcatcac cgagtcgagg agggcagaca tgcctgatcc tccttgttga
    10561 tgatcgcctg caaggccggc tccagcgtcg gataacggaa ggtataaccc ccggctctcg
    10621 ccttcgccgg atcgacacgt tgcccggtgg tcatcaacgt ccccaactca cccagcgcca
    10681 gatgcaaggc gagaccggga accggaagcc acgagggacg gtggagcact cgccccacgg
    10741 tggcgcagaa cgtcttcatg gtgaccgcct caggcgccac tgcattcaca ggacctgaga
    10801 tcgtcgccgt cgcgatgacc cattggatga gtccgatgag atcggaccgg tgaatccatg
    10861 acacccattg cgtgccagga agcaccggcc ctccggcaaa gagccgaaac ggcagcaaca
    10921 tcttcggcaa ggcgccgcca ccctgttcga gcaccatccc ggttctcaac agtacgacgc
    10981 gcgtcccgag ctgaccggcg cgcaaggcct ccgcttccca agcggcagaa agctcggcga
    11041 gaaagccttg accgatcgac gacgcctcat ccagaagccg atcgtcgctc gggccgtagt
    11101 aaccgatccc cgatgcgctg atcaacagag gaagggacgc agaccggccg gccactgcgt
    11161 ctaccagcag tctggtcgac agaatccggc tctccaccag aagccgcttg cgggcgtccg
    11221 tccatcgccc atctgcgatc ggcgcaccgg caagattgat gacgacatcg gccgtcgcca
    11281 cttcagagtg ccagggtccg gcagaacagg cgtcccattc cacgtaccgg accgacgact
    11341 gagacgcatg accggaccgg cggctcaaga ccaccacctc atgaccctgt cgggccagct
    11401 cttcgaccaa cgcatggccg atgaatcccg ttcctccggc gacgatgatc ttcatctgtc
    11461 cgtcctcccg tctggcacag tccacggcta cgatgccccg tcctggtcgg cctcatcggc
    11521 cgactcctcg tcactctcgt ccggagctcg atcatccgat gactgtttca caggcctgct
    11581 gccggtaacg gccgtcacgc gctgactgaa ctttcgctgc ttcgtgccat acagcaggtt
    11641 cggcggcggg ctccggcgat tcttcaggat gaccgggtcg agcacctcgc cgcacatcgt
    11701 gcaatgccat gccataatat cgatctgacc ggtatcatca agcaaatcga tgtagtgctg
    11761 tgtcaacaac aatcctccgc aacgcgggca gatgtgatgt ccgggctgcg gagtggaaag
    11821 agattctggt gtcccatgtg tttccattgg aacccccgac tgggaaaaaa atggacgacc
    11881 acgacctgaa ccgcgtccac cgcatacgac tgctcccgtt ccggtgccca cccataccct
    11941 attgcaccct cgaccgttcc ggccgagccg gccgcacaac cacataggca gcgcgccgct
    12001 gcctgacgaa cagggccgcc ccgatactca ccaccatcgc cgcgatgcca tatgcgatca
    12061 tctgggtcat gaatacctcc tgatcatggc gcctgggcgg cgccgtactc tctccgcgtc
    12121 accagctggt tcgctgaggg gacggattcc tccgaaggaa gaacccgccc acctcaacgg
    12181 gaggccgcgt gcggagtcgc ggtacctcct cctcttcgct gcttcggacc cgcctcattt
    12241 cctcgcgcct gctcatggct tatttcgctg tctctccacc ccggacacac cggaaaccgg
    12301 tgccatgtgt ttgcagcgcg aatcctccgc gcccccgtgc cgtcgcggtg atatccgact
    12361 gggggctgtg ccacgatcct ccacgaataa cccggacgat ccccttgtcg ggtccctgcg
    12421 ggttcttgtc gggagcattc gcgtaatagt cgggggcgta ccaatcctgc acccattccc
    12481 gcgcgttgcc ggacatatct ttgatgccgt acggcgactc gcctccggac aacgaaccga
    12541 cggcgtgcaa cgtcttgtcg tcgacccact cccgatcgaa gttcgcccgg gtcggactcg
    12601 gggtctcgtt cccccagggg aacatccggc catcggtgcc gcgcgcggcc ttctcccact
    12661 ccgcttcggt gggcagtcgt ttcttggccc acgcacagta ggccttggcg tcgtaccacg
    12721 tgacctgcac gaccggcagc tgctcgatcc ccttggccga gaccaggacc ccggtgccgt
    12781 aggggttcgg cggactacga tgaccggtgg tggcgacaaa cgtcatgtag cgctcattgg
    12841 tgacttccac ctgatcgatg gcgaacgtat cgacatgaac cgaccgttgc ggccactcgt
    12901 cggctcgccc cttgggattc tcactgccca tcagaaacgg gccgcccgga acggtgacca
    12961 tcggaaccgg atcaagttcc ttgagagtcg gcgccgcgag cacaggtgtc agggcgaccg
    13021 cacaggctgc cgtcacgaac aggctcatgc atctccacct ggttcttctc ctggtcaaac
    13081 cttgagtcat ttcttctgct ccttgctccc cgcgattgcc tgggccgtat gaatcttgat
    13141 ctcaccgaca agccgttcca ccttgcccgt gtctccccgg tcagccgcct cctgcgcttg
    13201 cgtcaccaga gttctggctt catgcagatg ctgctcgatt tgcgcctgca ggtcaggcga
    13261 cgccacggta ccccccaggg ccgcgcggtg atacacctcc cacgcgtgat ccacgctgtg
    13321 ttccgcctca tgcaccatct ggagtttctc cggatggggg tggcggctgg catcgattgg
    13381 accggcttgg agatcccccg tcatcaagag cgcggtgaat ccgccgacca atgtgatcat
    13441 cgtactgtac ctcatgcctc tgcctccgtc gggtgtatgg cgggccagcc ccgcccgccc
    13501 tgtgcactca tcgctgcttc atcgactgtt cgatgagagg tcgcagctcc aacaattcaa
    13561 cgttcaccgg atccatggcc aaaccccggt tgacggcctg ctgtgcctcg ctgaaccgtt
    13621 cccgccccat ttccaccaac gcggtgatgt agctatctct gatgcgctgc tcccagtcgg
    13681 ccggtgccgt cttcgcgcac cgaaagccga gaccgacgcc gtagctgtta ttgagcggat
    13741 gattccagaa ccggtgcgtc gagcgaatcg tgccttccgg cgcaatccag gagccgccac
    13801 ggatgacccg tttctcaccg acggtcaagc ggtctacgta ggttccggaa ccaccgatcc
    13861 acagaggttt ctccgggccg gtgggattca ccatggtctc gagtctcccg taatacttcg
    13921 ggtcatacca gtcgccgacc cactcgaaga cattgcccgc catgttgtgc acgccgtagt
    13981 agctggcacc ctccggatgc gaatccaccg gcaaggtcgc atcatgatgc ttgtcataat
    14041 tcgccttgtt ggaatcgaag tcgttgcccc agggatagag attgccgtgc gggccgcggg
    14101 ccgccttttc ccattcggct tcggtgggca accgcttccc ccgatattcg cagtaagttt
    14161 tggcatcgat ccaattgacg ccgaccaccg gctgctgcgg cttgttcagt cggggatcgt
    14221 cccagtaggc cggcgctgga tgaccggttg ccttgatgaa ggcgccatag tctttgttcg
    14281 acacctcgta c
//
LOCUS       DAORRQ010000080        13944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_218_length_13944_cov_204.906977, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000080 DAORRQ010000000
VERSION     DAORRQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13944
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218_length_13944_cov_204.906977"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK224_17920"
     CDS             complement(<1..205)
                     /locus_tag="PK224_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17679.1"
                     /translation="MKAVAFAAVTSLVLLFVWERVDIVRIGYHIERLKAQKVLLERER
                     DELRVKISGLTAPERIARLASDKL"
     gene            complement(202..1131)
                     /gene="rsmH"
                     /locus_tag="PK224_17925"
     CDS             complement(202..1131)
                     /gene="rsmH"
                     /locus_tag="PK224_17925"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRB17680.1"
                     /translation="MEIGSARDSHEPVLVEEILFWLQCKPGGVYVDCTLGYAGLAARL
                     LDRTAPDGILVGIDRDAAALAESQMRLREVAHRVHFRHGNFCDLKALSMGSGVSQVDG
                     VIFDLGVSSPQLDRAERGFSFREDGPLDMRMDQREGRTAADLVRDLPETELADLIYQL
                     GEERYSRRIARAIVQARMQGVIGTTWQLAAVVERSVPASYRHGRIHCATRTFQALRIA
                     VNRELDVLEPALRDAVDILAPGGRVCAVSFHSLEDRIVKHTFRSLANGSEASVRVLTK
                     KPVTASESERSRNPRSRSAKLRVVERISKEYVK"
     gene            complement(1436..2728)
                     /gene="asnS"
                     /locus_tag="PK224_17930"
     CDS             complement(1436..2728)
                     /gene="asnS"
                     /locus_tag="PK224_17930"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRB17681.1"
                     /translation="MPVMYIDEAAAHAGQEVTIRGWLRSRRDKGKLHFLIVRDGTGDI
                     QAVVSKATVGDAQFARSAELTQESSLVLTGTLRQDARAPGGYELDVLRLEVLQVAEPF
                     PIQPKEHGTGFLMEHRHLWLRSSRQHAVLRIRHEIIRACRNFFDDRGFTLVDAPIFTP
                     NACEGTTTLFQTRYFDETAYLTQSGQLYSEATAAAFGKVYCFGPTFRAEKSKTRRHLM
                     EFWMVEPEVAFAQLPDMMDLAEQFLSHIVAAVLKSRRAELLLLERDPAKLEHVTAPFP
                     RISYEEAIDILQRRGNPAKPGDDFGADDETILSNEFDRPVIVHRYPAALKAFYMETDP
                     ERPDLALCMDVLAPEGYGEIIGGGQRIHSHEKLLGRIQEHHLPVEAFQWYLDLRRFGS
                     VPHAGFGMGIERAVAWICGLDHVRETIPFPRMLYRLYP"
     gene            2864..3958
                     /locus_tag="PK224_17935"
     CDS             2864..3958
                     /locus_tag="PK224_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17682.1"
                     /translation="MTHTTLLHHPRSRTPLLAALLLMGLLPSALAGEFRTDNPLNRPG
                     KIYWCPDRTADKRITATPDAGCTALYDEERDAKKPEKPDPPDHDASTPKEPLKIVDIQ
                     NEASKFTREYREFLECCAGEFDSVERIDEFLDQSNHILMSVQQKGIYNSAGFGVGSGA
                     GGLGGGPGQSPKLGTFARQWTLSEIVGTVAIARDDLMKIKTRIKALGERFEKLDSLDY
                     EQRGREARRIDEEREAIAHDVRAKRPPASAPTGMDIQDTTIPTRIGGDIENTHLNRSL
                     NPSFGADIGATVSPYSNVNESLRPRRGEDLKDSNLPDRVGTHTQDTTLPNAFGFDINS
                     AENGERSSSVQTRGVGAEIGDSSLNNQSRR"
     gene            complement(3986..5005)
                     /locus_tag="PK224_17940"
     CDS             complement(3986..5005)
                     /locus_tag="PK224_17940"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17683.1"
                     /translation="MAKRLICLLFFVWSLGGCTGSDPIVVIQLHPTNPDILYIATNDY
                     IYKSRDGGKTWSNLSKGMSHSRVIAMAIDPAYPATIYAGTKGDAVYKSYDGGQRWTSM
                     RGGLDDATISSVVNQFVFDPADDTHIFLATTMGVFESRNAGERWTKRMDGMKEVLMVV
                     TLGMDPTRSSVLYAGTSGGVYKSVNQGAYWQQVNHGLVPEGMVKTSRALSVTSVQVDP
                     FTTETVYAATLAGLYKTTDGAASWVRIGESLQDQMIVSMVLDRARPGVMYVAARDGIH
                     RSDDGGKSWVALNEGLATTNIRSLAQSALDPQLFYAGTNGSGLYRSTDGGAHWEALPS
                     VFPKE"
     gene            complement(5153..5650)
                     /locus_tag="PK224_17945"
     CDS             complement(5153..5650)
                     /locus_tag="PK224_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRB17684.1"
                     /translation="MADQSSFDVVSEVNMQEMKNVVDQATKEIKQRFDFKDSKTELTL
                     KEKEKELVVLSDDEYKLNAVIEIIKTKCVKRGVSLKAFEYGKIEEALGATVRQVIKIQ
                     SGISSEKAKEITKAVKESKIKVQAQIQGEQVRVISKSKDDLQTAIAFLKGKDFGIDLQ
                     FTNYR"
     gene            complement(5689..7695)
                     /locus_tag="PK224_17950"
     CDS             complement(5689..7695)
                     /locus_tag="PK224_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRB17685.1"
                     /translation="MKMVYRLLWALLSVLGAVALAHVAGLVNPHEKVNGLWLVVAAAC
                     IYVLAYRFYGRWLARHVVQLDDTRLTPAVRLNDGVNFHPTNRVVLFGHHFAAIAGAGP
                     LLGPVLAAQFGFLPGFLWLVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHDELGSIT
                     GTATAVAVLFIVVVALAGLGFAVVNALYHNAWGTFTIAMTIPIGFIMGFYLQKFRPGA
                     VAEVSVIGVVLLVAAVLFGRVVGQSSLAWLFEFEKPALVWLLAGYGFLASVLPGWMLL
                     VPRGYLSTFMKLGVVFLLGFGVILMAPTIEMPRVTAFANGGGPIIPGTLFPFLFITIA
                     CGAVSGFHALVSSGTTPKMIEQESQAVVGYAAMLLESFVGVMALIAASVLIPGDYLAI
                     NTMLPAETLTAMGFAPSRIAELSQLVEVNVAGRPGGAVSLAVGMASIFSALPGMAGLM
                     AYWYQFALVFEALFILTTIDTGTRVARYLIQEMAGRVYAPFRQMNWWPGVLLSSAFVV
                     GSWAYLIGTGSISTIWPMFGAANQLLGTLALCIGTTVLIKMWKSPYLWVTALPMLFVG
                     GITLAGSYEMFWMFLAKAATLAAGQAFALYLDAVLVAVVAVLGLVVLSDSMKQWYGYV
                     ILKRPFSSSEVMATAGGGSAGRVQTAIHRHDERVFKLPPGGGCC"
     gene            complement(7853..8218)
                     /locus_tag="PK224_17955"
     CDS             complement(7853..8218)
                     /locus_tag="PK224_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17686.1"
                     /translation="MFLQVVALKTHDVSDQQLPVLGGHPDEAGVEAVRTAWDRKGIKP
                     QYLDRRCEGNLLGEQQCEGKDFTGFVTLSDRHADTAATEVDRFLSALTCRVVGLVPNA
                     DGQKHGDAIKFAAFSPGRL"
     gene            8374..9381
                     /locus_tag="PK224_17960"
     CDS             8374..9381
                     /locus_tag="PK224_17960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB17687.1"
                     /translation="MRGLKWFLGLLVALATIGALYQIVGMVLDRKQHPPIGRLIDIGG
                     HRLHLYCIGQGSPTVVLEAAAPGWSLYWSTVQPEVAQVTRVCAYDRAGLGWSERGPLP
                     RTGRRLARELHQLLTRGGIPGPYILVGHSLGGFVTRLYREEHPLDVVGMVLVDAGHES
                     EMRQAEFRSFANAGKSMLPVIRAMTILGIPRLMASYDQLPPLLTGQEEKVPAEIRPML
                     RAGWLRTGYFATLTDESDALIETLEQVRHTGSLGDLPLVVVTATGPLWWPDMPGQVNP
                     AKFRKMWLDLQQNLTTLSSNSRQVFADQSSHFVQFDQPTLISETIRQMVIATRLAPRT
                     K"
     gene            complement(9431..11011)
                     /locus_tag="PK224_17965"
     CDS             complement(9431..11011)
                     /locus_tag="PK224_17965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRB17688.1"
                     /translation="MAAGASPAAGSVVERLDSASSVVDGVVAGFLFLFVFGSGLIHIE
                     SFPPLWFDEGWTVCVARTWVELGHYGCLLRGEPAPPSLAAHFPVVASVAASFTLFGVG
                     VWQTRLVGLLYTLGAFLLLYALARRLYGRSIAIAALALLLLVPLKWSIHPLYIGRQVL
                     GEMPLLCFLLAGYVCFLRSTHRPLWQAATIGCWALAWMTKAQVAPFLVASIAGTMVVM
                     SLRGDWSVVGRLAVAMIGSWGGCRLLLYAKDWLLAGHIMPHPPVDGMTEAIALVFVPS
                     IRLETIRYLFVSWPEYPLGLAYAAWRVGGTSGSVAKVSVEQTVQTMLLLLAGSWLAWF
                     AFLSAGEPRYAMPGLFLAAPFTAALFHDFTRGFDVAFLTRTLTGLLRSRHVTRQGIKA
                     VVVVMLLVMMGWVAVQERYAFRARADDRDLFAVTHYLNTATPPTALIETYDSELFLFL
                     HRPYTYAPPRALVEIIRREQGEAKAVTYDPLESRPDYLVVGEYGRWAGFYKPLIAQNR
                     VRLVTSIGRYQIYEPVRR"
     gene            complement(11469..12803)
                     /locus_tag="PK224_17970"
     CDS             complement(11469..12803)
                     /locus_tag="PK224_17970"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRB17689.1"
                     /translation="MTRMQLRTKAWFGDEDLTIDFPASWKLVEIGPRDEPALTVEAMR
                     ECLSRPIGTRRLSEIARGKRNAVIMVDDLTRPTPGADLLPLLVEELTSGGIPAHDITV
                     MLAGGTHPPALADERAKKVGSRLSSAVKVLAHDSRGDLVNCGRSPAGLPLHINRRVME
                     SEVKIGVGCIYPHPVAGFSGGAKIILPAVCGVETTRMMHDYLRGSRERGGSIQTELRQ
                     DMVAVARRVGLDGIVNVTLNRHRAICGLFAGDVVQAHEAGVRAAQRLYAVPMCPEAEV
                     VVADMYPFDTSWQFAQDRGMWPVEQATGEVSRVVIAACPLGMGTHELFPVDSPLWSRI
                     ARRLSHFRLADLDRPLEKLRTVAQLIRRKQHHLMVLSPGLHGQDLTSMFPHAQRFGDW
                     PTLRAALLARHGEGPVTVAVYRCAPFLIPTAAVLGQEVGVSEPTLTRQPTSA"
     gene            complement(12800..13714)
                     /locus_tag="PK224_17975"
     CDS             complement(12800..13714)
                     /locus_tag="PK224_17975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB17690.1"
                     /translation="MPLDRHKISVIVPTVGRDSLALCRTALERQTRPPDEIVIVVDEF
                     RRGVVWARNEGIARSSGDVIAFADDDVIPPADWLERLVGALDRCDAAAAGGTFQETDP
                     LLDAIRRRNPFPEREQMDHGGLVGNSGNLMIRRQWLARCQQEDGYVFNPCFGGSGEDW
                     ELMWRLRKRGARMVYVPSQVQHLRRAAVGQHLRHSFQRGVGIARLFLVMRCDQSGVVP
                     QDSLLWGTRGRRTDPRWFQALWTKLIGPFDGKGFQSRQQFWVFWLGEKCQAAGFLWEL
                     VTGLLSKRLMPRRAVSQSAGSAAIGPKL"
     gene            complement(13722..>13944)
                     /locus_tag="PK224_17980"
     CDS             complement(13722..>13944)
                     /locus_tag="PK224_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17691.1"
                     /translation="VAAWVTPPLLSILVGAVVGTVLYWGGVFRLGLVQVEEIELMVRS
                     LPGAFRLTGERLFRYLAPVLLRLKAAAWS"
BASE COUNT         3123 a         4588 c         3767 g         2466 t
ORIGIN      
        1 ccaacttgtc gctggccaga cgcgcaatcc gctcaggtgc cgtgaggccg gagatcttca
       61 cgcgcaattc atcgcgctcc cgctcaagga ggaccttctg cgccttcagc cgttcgatgt
      121 ggtagccgat gcgaacgatg tccacccgct cccacacaaa gagcaacacc agcgacgtga
      181 ccgcggcaaa tgcgacggct ttcatttcac atactccttg gagatccgct ccacgactcg
      241 caactttgcg ctccgtgacc gcgggtttcg gctacgctcg ctttccgagg cggtgaccgg
      301 ttttttcgtc aacaccctga ccgacgcttc ggagccgttc gccagcgacc gaaacgtatg
      361 cttcacgatc ctgtcttcga gcgaatgaaa cgagacggca cagacacgcc cgcccggcgc
      421 cagaatgtcc acggcatccc ggagcgccgg ctccaacacg tccaactcac gattcacggc
      481 aatccgcaac gcctgaaagg tgcgcgtggc gcaatgaatt cgtccgtgcc gatacgaagc
      541 cggcaccgag cgttccacca ctgccgccaa ctgccaggtt gttccgatca ccccctgcat
      601 ccgggcctgc acgatcgcgc gggcaattct gcgcgagtac cgttcctcac cgagctgata
      661 gatcagatcg gccaactcgg tctcgggcag atcgcgaacc aggtctgcag ccgtgcgtcc
      721 ttcgcgctga tccatgcgca tatccagcgg gccgtcttcc cgaaaactga aaccgcgctc
      781 cgcacgatcg agctgaggcg aggacacgcc cagatcgaaa atcacgccat ccacctgcga
      841 caccccgctc cccatactca acgctttcag gtcgcagaaa tttccatgtc ggaaatgcac
      901 ccgatgcgca acctctcgca gacgcatttg cgactccgcg agtgccgcgg catcgcgatc
      961 aatccccaca aggatgccat ctggagcggt gcgatcgagg agacgagcag cgagccctgc
     1021 atatccaaga gtgcagtcca cgtaaacacc acctggttta cactgcaacc agaacaagat
     1081 ctcttcgact aaaaccggct catgggaatc gcgagcagaa cctatttcca tttccaccca
     1141 cttcacccca ctcattgggt ggagtataca cccaaccaaa tgactgtcaa cagaattaac
     1201 ccctatttat caagaatatt gttcagttta gacacatgtc taatttggcc ggaatttgcg
     1261 ggaaagttat ccacatttgt ggacaagtct gtgggaaact cgcccacctg acaattatct
     1321 tcgggcaaat ttctgtcagg gaaaatgggg gtcatcaaaa aatcagatgg atgaaatacc
     1381 tgcatttcgg tggtcgctcg cgcagaaatg caggcagaaa cggaaggcct cgccgctatg
     1441 gataaagtcg atacaacatc cggggaaagg gaatcgtttc ccgcacgtga tcgagcccac
     1501 aaatccaagc cacggcacgc tcaataccca ttccaaaccc cgcatgcgga accgagccga
     1561 accggcgaag gtcgagatac cattgaaacg cctcaaccgg tagatggtgc tcctgaatgc
     1621 gtcccaaaag tttttcgtga gaatggatgc gttggccacc gccgatgatt tctccatagc
     1681 cttccggcgc cagcacatcc atacagaggg ccagatcagg ccgctccgga tccgtctcca
     1741 tataaaatgc tttcaaggcc gcagggtatc gatgcacgat gaccggtcga tcgaattcat
     1801 tggacagaat cgtctcgtca tcggcaccaa aatcgtcacc gggcttcgcg ggatttcctc
     1861 tccgctgcag aatgtcgatg gcctcttcgt agctaatgcg cggaaacggc gccgtcacat
     1921 gctccagctt cgccggatcc cgttcaagca gcagcagctc ggcgcgacga ctcttcagca
     1981 ccgccgccac aatatgagac aggaactgtt cagccagatc catcatatcg ggcaattgcg
     2041 caaaggccac ctccggctcc accatccaaa attccatcaa atgccggcgc gtcttggact
     2101 tctccgcgcg aaaggtcgga ccgaagcaat acacctttcc aaacgccgcg gctgtggcct
     2161 cactatagag ttgcccgctc tgagtcaaat aggccgtctc atcaaagtac cgcgtttgaa
     2221 ataaggtggt cgtgccctca caggcattcg gcgtgaaaat cggcgcatcg accagcgtaa
     2281 aaccacgatc gtcaaaaaag ttccggcagg ctcgaataat ttcatggcga atgcgcagca
     2341 cggcatgctg gcggctggag cgcaaccaga gatggcgatg ctccatcaag aatcccgtgc
     2401 cgtgttcttt cggttggatg ggaaagggct ccgctacctg caacacttca agacgcagca
     2461 cgtcgagctc atacccgccc ggcgcacgcg catcctgccg taacgtgccg gtgaggacga
     2521 gactggactc ctgggtgagt tccgccgatc gagcaaactg tgcatcaccc accgtcgctt
     2581 tgctcacgac cgcctgaatg tccccggttc catcccgcac gatcagaaaa tggagctttc
     2641 ccttgtctcg acgactgcgg agccaccccc gaatcgtcac ttcctgtccg gcatgggcgg
     2701 ccgcttcatc gatatacatc acaggcatgg gtcatcctat tcaaatagag gtgcgccgga
     2761 gaggtctatg gccggagggt aacgaacagt cgcgggcttt tcaagcgcat cacaaatccc
     2821 ccctttcgtt gacacaatgt catgccgggg gcatcatcac atcatgacgc acaccacatt
     2881 gctccaccat ccccgatcac gtacaccgct gctcgccgcg cttctcctga tgggcctgtt
     2941 gccctccgca ctggcaggtg aattccgaac cgacaacccg ttaaaccggc ccggaaaaat
     3001 ctattggtgc ccggaccgca cggcagacaa gcggatcacc gccactcccg atgcaggctg
     3061 tacggccctg tacgatgagg aacgcgacgc gaagaaaccg gagaagccgg acccgcctga
     3121 tcatgacgcc tccactccga aggagccgct caagatcgtt gacatccaga atgaagcgtc
     3181 gaagtttacc cgcgaatatc gagaatttct ggaatgctgc gccggcgagt tcgattccgt
     3241 cgaacgcatc gacgaatttc tggaccagtc gaaccacatt ctgatgtcgg tgcaacaaaa
     3301 aggcatttac aacagtgcgg gcttcggcgt gggaagcggg gcaggagggc tcggcggcgg
     3361 ccccggtcaa tcgccgaaac tcggcacctt tgcccgccag tggacactca gcgagatcgt
     3421 cggaaccgtc gccatcgctc gggacgacct catgaaaatc aagacgcgca tcaaggccct
     3481 tggagagcga ttcgagaagc tcgattcatt ggactatgag cagcgcggga gagaagcacg
     3541 gcgcatcgac gaggaacgcg aagccattgc ccatgacgtt cgagccaaga ggccaccggc
     3601 ctcagcgccg acaggcatgg acatccagga caccaccatc ccgaccagaa tcggcggaga
     3661 catcgagaac acccatttga atcgatccct caatccaagc ttcggcgccg acatcggagc
     3721 cacggtgtcg ccgtacagca acgtcaacga atccctgcga ccccgcagag gagaagacct
     3781 caaagacagt aacttgccgg accgggtggg cactcacacc caggatacta ccctgccgaa
     3841 tgcattcggc tttgacatca attcagccga aaacggcgaa cgctcgtcgt cagtccagac
     3901 gcggggcgtc ggcgccgaaa tcggcgactc ctccctcaat aaccagtccc gccggtagac
     3961 tcgcgccggc cggatctaca cccggttatt ccttgggaaa gaccgatgga agcgcctccc
     4021 aatgcgcccc tccgtccgta ctccggtata atccactgcc gttcgtgccg gcgtagaaca
     4081 gctgtggatc cagcgcgctc tgcgccagcg accggatatt ggtggtcgcc aacccctcgt
     4141 tcaatgccac ccagctcttt ccgccatcgt cgctccggtg gatgccatcc cgcgcagcca
     4201 catacatcac tccaggccgc gcacgatcga gtaccatcga cacgatcatc tgatcctgca
     4261 acgattcgcc gatgcgtacc caggaagcgg cgccgtcggt ggttttatag agaccggcca
     4321 gggtcgccgc atagaccgtc tccgtcgtga atgggtcgac ctgcaccgac gtgacgctca
     4381 gtgcgcgcga tgttttcacc atcccctccg gcaccaaacc atgattcacc tgctgccaat
     4441 acgccccctg attcaccgac ttgtagacac cgccgctggt gccggcatag agcacggatg
     4501 atcgcgtggg atccatgccc agcgtgacca ccatcaacac ttccttcatt ccgtccatcc
     4561 gcttcgtcca ccgctcaccg gcattcctcg attcgaacac ccccatggtc gtagccagaa
     4621 agatatgagt atcgtctgcg gggtcgaaga caaattggtt cacgaccgag gaaatcgtgg
     4681 cgtcgtcgag cccgccacgc atggacgtcc agcgctgccc cccgtcgtag ctcttgtata
     4741 cggcatcgcc ttttgtcccg gcataaatcg tcgcaggata agcggggtca atcgccatcg
     4801 caatcacgcg cgagtggctc atccctttcg agagattgct ccaggtcttg ccgccgtcac
     4861 gcgacttgta aatgtagtcg ttcgtggcga tgtagagaat gtccgggttg gtgggatgga
     4921 gctgaatgac gacgatcgga tcactgccgg tgcatccgcc aagggaccat acgaagaata
     4981 aaagacagat gagacgtttg gccatgctgg aactagtggc gactgtatgg gatggtcaaa
     5041 caggtcgccc agcaaggccg cagcgagaga aggggcgagg cgtactgtgc ttgtacgtcg
     5101 agccgctgat cgacgcgaga acgaagctgg aggcctgttt caccatccca ggctagcggt
     5161 agttggtgaa ctgcagatca atcccaaaat ccttcccctt gaggaaggca atcgccgtct
     5221 gcagatcatc tttactcttg ctgatcacgc gcacctgctc gccttgaatt tgcgcctgca
     5281 cctttatctt ggactctttc acggccttgg tgatttcctt ggccttctcc gatgagatac
     5341 cgctttgaat cttgatcacc tgccggactg tagcgccgag cgcctcctcg attttgccgt
     5401 actcgaacgc cttcaacgag accccgcgct tcacacactt ggtcttaata atttcaatga
     5461 ccgcattgag cttgtactcg tcgtcggaga gcaccaccag ttccttttct ttctccttca
     5521 gcgtcagctc ggtctttgaa tctttgaaat cgaagcgttg cttgatttcc ttcgtcgcct
     5581 gatcgacgac gttcttcatc tcctgcatat tcacttccga caccacatca aacgatgatt
     5641 ggtcagccac gatgccctcc tcctgctgtt tcgtcaggtc ttccgcccct aacagcatcc
     5701 tccgccaggc ggcaatttaa acacgcgctc atcatgccgg tgaatcgccg tctgcacacg
     5761 accggccgat ccaccgccgg ccgtggccat cacttcactg ctgctgaacg gccgcttcag
     5821 aatcacatac ccataccact gcttcatgct gtcgctcaag acgaccaggc ccaacaccgc
     5881 gaccaccgcc accaacacgg catcgagata caatgcaaag gcctgccccg ccgcaagtgt
     5941 cgccgccttc gccaaaaaca tccaaaacat ctcatacgag ccagccagcg taatgcctcc
     6001 cacgaacaac atcggcagcg ccgtcaccca gagatagggc gacttccaca ttttgatcaa
     6061 caccgtcgtg ccgatacaga gcgcgagcgt gcccagcaat tgattggctg cgccgaacat
     6121 gggccagatg gtcgaaatgc tgcccgtccc gatcagatag gcccacgacc ccaccacaaa
     6181 ggcgctgctc aacaacacgc ccggccacca gttcatctgt cgaaacggcg catacacccg
     6241 cccggccatc tcctgaatga gatacctggc cacacgggtc cccgtatcga tggtcgtcag
     6301 aatgaacagc gcctcgaaca cgagggcgaa ttgataccaa taggccatca gcccggccat
     6361 gccggggagc gccgaaaaga tggaggccat gcccactgcc aacgacaccg cccctcccgg
     6421 ccgccccgcc acattcactt caaccagttg cgacaactcc gcgatgcggg aaggcgcaaa
     6481 acccatcgcc gtcaacgtct cggccggcaa catggtgttg atcgccaggt aatcgccggg
     6541 gatcagcacc gacgccgcaa tcaacgccat cacgccgacg aagctttcca gcaacatcgc
     6601 cgcataaccc accactgcct gcgactcctg ctcgatcatc ttcggtgtcg tgccggagga
     6661 caccagcgca tgaaagccgg acacggcccc gcaggcaatc gtaataaaga gaaacggaaa
     6721 caacgtgccg ggaataatcg gcccgccgcc attggcgaag gcggtcacac gcggcatctc
     6781 gatcgtcggc gccatgagga tcaccccgaa gccgagcaaa aacaccacgc cgagcttcat
     6841 gaaggtggaa agatagccgc gcggcaccaa cagcatccag ccgggcaaca ccgaagccag
     6901 gaaaccatac ccggccagca accagaccag cgccggcttc tcgaactcga agagccaggc
     6961 aagagacgac tgtccgacca cacgcccgaa gagcaccgcc gccaccaaca gcaccacccc
     7021 gatcaccgac acttcagcca ccgcgccggg gcggaacttc tggagataga aacccatgat
     7081 gaacccgatg ggaatcgtca tggcgatggt gaaggtaccc caggcattgt ggtacagcgc
     7141 attcaccacc gcgaaaccca gtcccgccag cgccacgacg acaataaaga gtaccgccac
     7201 cgccgtcgcc gtcccggtga tggagcccag ttcgtcatgg gcgatttcag gcaatgaacg
     7261 gccgttgcgt cgcatcgaag cgacgaggat gatgaagtcc tgcaccgccc ccgccagcac
     7321 cgcgccgatc acgagccaga gaaaaccggg aagaaagccg aactgcgccg ccaacaccgg
     7381 tcccagcaac ggcccggctc cggcaatcgc cgcaaagtga tggccgaaca agacgacccg
     7441 gttggtgggg tggaaattga cgccgtcatt caaacgcacg gcgggagtca gacgtgtatc
     7501 gtccaactgg accacatggc gagccagcca acgaccataa aaccgatagg ccaacacata
     7561 gatacaggcc gcggcgacca cgagccatag tccgttcact ttttcgtgag gattgacgag
     7621 gccggcgaca tgggccaagg ccacggcacc tagaacagag aggagcgccc agagcagacg
     7681 atacaccatt ttcatgcgcg gcatcctagc ataaacggca ggagatggga gcgacgcgga
     7741 cccaacccgc atcgttcaga agctgggagg cgaagaggcg cagcgtcatg ctgaagggca
     7801 aggtgcggga cgactttgtt atactgctta caccatgccg cccgacatgc ctttacagcc
     7861 gtccgggaga gaacgccgcg aattttatcg catcaccgtg cttctgccca tctgcattcg
     7921 gcacgagacc gacgacacgg caggtgagtg cactgagaaa tcggtcaacc tcagtggcgg
     7981 cggtgtcggc gtgacggtca ctgagagtta caaacccggt gaagtccttt ccctcacact
     8041 gctgctcccc gagcaagttc ccttcacatc ttctatcgag gtactgcggc ttgatcccct
     8101 tccggtccca ggcggtgcgt accgcctcca cgcccgcttc gtcaggatga ccacccagaa
     8161 ccgggagctg ctgatcagat acatcgtgcg ttttcagcgc gaccacctgc aggaacacta
     8221 ttccgtctga agcgcagaga aggcttggcg gcttggcagc ccccgcacac gagggcagag
     8281 atgttcattg ggagtgccgc tcgcccggtc ttgggcgaca cttcgactcg tcccggtttg
     8341 cttcttccat ccatgacagg tgatcatgtc cccatgcgtg gactcaaatg gtttctcggc
     8401 ctgttggttg cgctggcgac aatcggtgcg ctgtatcaga tcgtcgggat ggtgctcgat
     8461 cggaaacagc atccgcccat cggccggctt atcgacatcg gcggtcatcg actccatctc
     8521 tactgcatag ggcagggcag tccgacggtc gtgctggaag cagccgcccc cggctggtcg
     8581 ctctactgga gcacggtgca accggaagtg gcacaagtca ctcgcgtctg cgcctacgat
     8641 cgcgctggcc tcggctggag tgagcgtggc ccgttgcccc gcaccggccg gcggctggcc
     8701 cgagagcttc atcagttgct gacccgcgga ggcatccccg gcccctacat tctcgtgggc
     8761 cattcgctcg gcggcttcgt cacccggctc taccgcgaag aacatcccct ggatgtcgtc
     8821 ggtatggtgc tggtcgatgc gggacatgaa tcggaaatgc gccaggccga atttcgatct
     8881 ttcgccaacg caggaaaatc gatgctcccc gtcattcgcg ccatgaccat actgggcatt
     8941 ccacgcctga tggcctcgta tgaccaactg ccgccgctgt tgaccggaca ggaagagaag
     9001 gtacccgcgg agattcgacc gatgctacgt gcaggttggc tgcggaccgg ctatttcgca
     9061 acgttgaccg atgaaagcga tgcgctgatt gaaaccctgg aacaggtgcg ccacaccggt
     9121 tcactcggcg atcttccgtt ggtcgtcgtc accgcaaccg gccccctgtg gtggccggac
     9181 atgcccggcc aggtgaaccc tgccaagttc agaaaaatgt ggctggatct acagcagaat
     9241 ttaacaacac tctcctccaa cagtcgccag gtgtttgccg accagagcag tcatttcgtt
     9301 caattcgatc agccgacgct catcagcgag accattcgtc agatggtcat tgccacccgc
     9361 ctggcaccac gtaccaaata gacatgaacc tatgcacgcg cgcggggagg aaatttttgc
     9421 ggcgtgatag ttaccggcgc acgggctcgt agatctggta gcgcccgatg ctggttacga
     9481 gtcgcacccg gttttgcgcg atcaacggct tgtaaaatcc ggcccatcgg ccgtactccc
     9541 caaccacgag atagtccggc ctgctttcca acggatcgta cgtgacggcc ttcgcctctc
     9601 cctgttcgcg ccggataatc tcaaccaggg cgcggggcgg ggcataggta tagggtcgat
     9661 gcaggaatag aaacagttcg ctgtcatagg tctcgatcaa tgccgtcggc ggcgtagccg
     9721 tattcaggta gtgcgtcacc gcaaacaggt cacgatcgtc cgcacgcgcg cgaaacgcat
     9781 agcgctcctg cacggcaacc caccccatca tcaccaggag catgacgacg acgaccgcct
     9841 tgattccctg cctcgtcaca tgcctgcttc gcaggaggcc ggtcagagtc ctcgtcagga
     9901 acgccacatc gaatcctctg gtgaaatcgt gaaagagcgc tgccgtgaag ggcgccgcaa
     9961 gaaacaaccc gggcatggca tagcgcggtt caccggcgct gagaaacgca aaccaggcca
    10021 accaactccc tgccagcagc agcagcatgg tctgcaccgt ctgttcgaca gaaaccttgg
    10081 caacggaacc ggaagttcca cctacacgcc aggccgcata ggcaagcccg agcggatact
    10141 ccggccacga cacgaacagg tatcggatcg tctcaagacg aatggacggc acaaacacca
    10201 acgcaatcgc ttctgtcatt ccgtctacgg gcggatgcgg catgatgtgg cctgccaaca
    10261 gccaatcctt ggcatacaga agaagccggc acccacccca tgaaccgatc atggcgacgg
    10321 ccagccggcc gacaaccgac cagtccccgc gaaggctcat gaccaccatg gtcccggcaa
    10381 tggaggcgac gagaaacggc gcgacctgcg ccttggtcat ccacgccagc gcccagcatc
    10441 caatcgtagc ggcctgccag agcggacggt gcgtgctccg gagaaagcag acatacccgg
    10501 ccaggaggaa gcacaagagt ggcatctcgc ccagcacttg tcgaccaata tacaggggat
    10561 ggatcgacca cttcagaggc acaaggagca gcagcgccaa ggcggcaatc gcgatagacc
    10621 gaccgtagag gcgacgggcc aacgcataga gcagcaggaa ggcgccgagg gtatagagca
    10681 atcccaccag tctcgtctgc cagacaccga cgccgaacag tgtgaaactc gccgccaccg
    10741 aggccaccac cggaaaatgc gccgccaatg agggcggcgc cggctcaccg cgtagcaggc
    10801 acccgtaatg ccccagctca acccaggtgc gcgcgacaca gactgtccag ccttcgtcga
    10861 accacagcgg agggaaactc tcgatatgta tcaggccgct gccgaacacg aacaagaata
    10921 agaatccggc aactactccg tccacaaccg atgaagcgga gtcgagccgt tcgaccacgg
    10981 agccggccgc aggtgaggca ccggccgcca cggagtcgaa ctcgttaggg cgtgcatcat
    11041 gaccgtcgtc aggcggtaac ggaatgtgag aggcgatagg catagcggat gaagttctag
    11101 caggctgcga aaaactcgat tactgcgcaa tatgcgacga tcaactcatg cgcggtgtcg
    11161 gtatgacaat gacccgcagg atgcgaaaaa agtccgtcca gcaaggccgc agcacgcgaa
    11221 gaggcgaacc gttcattcgt acccgcccac cccgagcctg ccaagacagg ctcttgtccc
    11281 gtggccgtac ggtgagcctc tgaggttcac gacgcgcgga ataacgcgcg tcacgtttgt
    11341 gaacgccgcc gagacggtga ggcggccgtg tcttgcgaga acgccgctgg cggacttttc
    11401 cgcatcctgc cagaaacgga ccggtcgtcg aagcatcgcg gaggacgaca tcctccgctc
    11461 cttccttttc aggccgaggt cggttgcctg gtgagggtcg gctccgacac tccaacctcc
    11521 tgccccagta cggctgcggt tggaatgaga aacggcgcgc atcgatagac cgccacggtc
    11581 acaggtccct cgccatggcg cgccagaagg gcggccctga gagtcggcca atctccgaat
    11641 cgctgcgcat gggggaacat ggacgttagg tcctgcccat gcaagcctgg cgacaacacc
    11701 atgaggtgat gctgcttccg gcggattaat tgggccaccg tcctcagttt ctcaaggggc
    11761 cgatcgagat cggccaggcg aaaatggctg agccgtctgg caatgcgcga ccagagcgga
    11821 ctgtctacgg gaaacaattc atgggtgccc atgccgagag gacaggcggc aatgaccacc
    11881 cgggagactt ctcctgtcgc ctgctcaacc ggccacattc ctcgatcctg cgcaaactgc
    11941 cagcttgtgt cgaagggata catgtccgcc accacgacct ccgcttccgg acacatcggc
    12001 acggcataaa gccgttgcgc tgcgcggacg cctgcttcat gtgcctgaac gacatccccc
    12061 gcgaacagac cgcagatggc gcgatggcgg ttgagggtga cattcacaat accatcgagc
    12121 ccgacccgtc gtgccacggc gaccatatcc tgccgcaact ccgtctggat ggacccgccg
    12181 cgctcgcgcg aaccgcgcag atagtcgtgc atcatccgcg tggtctccac gccacagacg
    12241 gccgggagga tgatcttggc gccaccggaa aaccccgcca ccggatgggg atagatgcag
    12301 cccacgccga tcttgacctc gctctccatc acccgtcgat tgatatggag cgggagcccg
    12361 gcaggcgacc ggccacaatt caccagatcg ccccgactgt catgggccag gaccttcacc
    12421 gccgatgaca gccgggatcc gaccttcttg gccctctcgt cggccagggc aggcgggtgc
    12481 gtaccgcccg ccaacatcac cgtaatgtcg tgcgcaggga tccccccgct cgtcaactcc
    12541 tccacgagga gtggaagcag atcggcaccc ggcgtcggcc tcgtcagatc gtcgaccatg
    12601 atcacggcat tgcgcttgcc cctggcaatc tcagacaacc tgcgcgtccc gatcggccga
    12661 gagaggcact cccgcatggc ctccaccgtc agcgccggtt catcccgggg gccgatttcg
    12721 accagcttcc acgacgcggg aaagtcgatc gtcaagtctt catccccgaa ccatgctttc
    12781 gtgcgcaact gcatccgggt catagcttgg gccctatcgc cgcagacccg gcgctttgcg
    12841 aaaccgctcg gcgaggcata agccgcttcg acaacaggcc cgtcaccagc tcccaaagaa
    12901 atccggccgc ctgacatttt tctccaagcc aaaacaccca gaactgttgc ctggactgaa
    12961 accccttccc gtcaaatggg ccgatgagct tggtccacag ggcctggaac cagcggggat
    13021 ccgtccgccg ccctctcgtc ccccacaaca ggctatcctg cggcacgacc ccgctctgat
    13081 cgcaacgcat cacaagaaaa agtctcgcga taccgactcc tcgttgaaac gaatggcgca
    13141 gatgctgccc gaccgccgct cgccgcagat gctgtacctg gctgggcacg tagaccatcc
    13201 tggcccctcg tttccgcagt ctccacatta attcccagtc ctccccggaa cctccgaagc
    13261 aggggttgaa cacgtaccca tcctcctgct gacatcgcgc cagccactgc cgtcgaatca
    13321 tcagattccc gctgttgccg accaatcctc cgtgatccat ctgttcccgt tcgggaaagg
    13381 gattacggcg acggatcgcg tccagcaacg gatcggtctc ctggaacgtg cctcccgccg
    13441 ctgcggcatc gcagcgatcg agggccccga ccagccgttc cagccaatcc gccggtggaa
    13501 tcacgtcgtc atccgcaaac gcgatcacgt cacctgagga tctggcaatg ccctcatttc
    13561 tagcccatac cacgccgcgt cggaattcat cgacgacgat aacaatctca tccggcggcc
    13621 tggtctgccg ctccaacgca gtccggcaca gtgccaacga gtctcgcccc accgtgggaa
    13681 cgatgactga gatcttgtgt cgatccaacg gcatagacca cctaactcca agctgcggcc
    13741 ttgaggcgca gcaacaccgg tgcgaggtac cggaacagcc gttcccctgt caggcggaac
    13801 gcgcccggca gactgcgcac catcagttcg atctcttcga cctgcacaag cccgagccgg
    13861 aagaccccac cccagtacag gactgtcccc accaccgccc cgacaagaat tgagagcagc
    13921 ggaggcgtca cccatgctgc aacc
//
LOCUS       DAORRQ010000081        13849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_224_length_13849_cov_83.348920, whole genome shotgun sequence.
ACCESSION   DAORRQ010000081 DAORRQ010000000
VERSION     DAORRQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224_length_13849_cov_83.348920"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK224_17985"
     CDS             complement(<1..228)
                     /locus_tag="PK224_17985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17692.1"
                     /translation="MSILIIDDCQEERDLLFTILHSAGFGPLLSVGTAREGLQLLGVG
                     KRGKQTSGIDLVLMDIEMPEIDGLEACQRIRM"
     gene            complement(267..1412)
                     /locus_tag="PK224_17990"
     CDS             complement(267..1412)
                     /locus_tag="PK224_17990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17693.1"
                     /translation="MTRLTARFTTTGLLVLLTGLIFLAELISEFRLASWVAYLLLPVP
                     ASRLYPRHMFLFAVGGWSLLMAAGCLTPLPTDEATNTLVSRTIGILGLWIIAYQLARQ
                     PAACGLQEVSRPAFPGTDHPRRLGPASQATEPVLPRVPAEPAEQKTRVLLVDDSIESH
                     TLMRSYFRNLPYDLEIAPDGEQAVATFQTGLFDFVLIDLHLPGLDGFTATRAMRAWEA
                     SHKHPPIPIVAVTASSGVESQARSLEAGCTDFLTKPITKARLLSTLRKYRRSFPGTGT
                     TPAQPAASPDLTERIDDEIRRRRPAFLANRRKDVAMMQDALGKGDYEAIRTTGHRMKG
                     LAGSFGFPDIGAVGHRLEQAAQSRDREAIRREIDRLTTILLGVDEAA"
     gene            complement(1587..2477)
                     /locus_tag="PK224_17995"
     CDS             complement(1587..2477)
                     /locus_tag="PK224_17995"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17694.1"
                     /translation="MRLTRLLLLTLCLFPAVGYQPSASAALRVDDMVMVPAGEFTMGA
                     SVESGGLPDERPLRLIYLGAFWIDRHEVTNAAYQEFVQATGYQAPANSAPALTLWEHN
                     APLPGIEQHPVVNVSWLDAVAFCRWANKRLPTEAEWEKAARGTDGRTYPWGNEWNFEN
                     GNSASYWARQTVQFADSTEWDAFWVKGVGAATSKERGLKGEILTLPVGSFPAGASPYG
                     ALDMAGNAAEWVQDWYNPNDYRTATLTNPQGPERGAIKAMRGGSWLKPAISLRTTDRD
                     WGTMDSRPSGTGFRCARDAY"
     gene            complement(2483..3235)
                     /locus_tag="PK224_18000"
     CDS             complement(2483..3235)
                     /locus_tag="PK224_18000"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HRB17695.1"
                     /translation="MAHKRLTRTQFLERLLAIMDRKHHWAWPIIMGPGITKAQLKLHY
                     QQEFLVYVRDFPVLLARVHGQNPPSDVRRMLAENIYEEDTGGLSFGRSHPELFNEMMR
                     GLGFDSETFRRTKLLPASARYRAWLEKVTASRDWVVGAAALAVFVEGSVKDRQEIQAP
                     SKPKTETEIEAYIDAHPLIRHHGIGRQHMDLIRAHQKVEAGHRQDAYTMVVNYAETGS
                     QQNAVLVCVTKGLALWTAYRDNVAKACKLKKA"
     gene            3283..3567
                     /locus_tag="PK224_18005"
     CDS             3283..3567
                     /locus_tag="PK224_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17696.1"
                     /translation="MKPITIQDSDQILNFLAEVALHGKGFTTDCLLDYVLDEGFTEPI
                     YLNASGEDPDAFYKDQPQAWAIYQVREWKRVLTVSGGAGKERRVQITETP"
     gene            3797..4066
                     /locus_tag="PK224_18010"
     CDS             3797..4066
                     /locus_tag="PK224_18010"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRB17697.1"
                     /translation="MPRHHPENSTKLVTLCESGDVGELALIKSLLDGNGITYTVQHEH
                     VGALYPGVALLGSRVMVEERDKQRAEVLLSRLAMHIRETTGDVDG"
     gene            complement(4105..4557)
                     /locus_tag="PK224_18015"
     CDS             complement(4105..4557)
                     /locus_tag="PK224_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17698.1"
                     /translation="MTDQTTSATPVSALPPAVEPTPFIRQRPVRIIIHTFLGLFVVAL
                     IAFRFVPGWLDPDFSKHIESHRVMVGMDRKQVLDSWGSPNTMNVTHTSDGLRREEWIF
                     EDWESPAVVKHRYLYFEEGKLIGGHFSGSDVRLPIPTTPEPVKPKGHP"
     gene            4727..5380
                     /locus_tag="PK224_18020"
     CDS             4727..5380
                     /locus_tag="PK224_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17699.1"
                     /translation="MGCWRYSINRCWIGLIMLGICLTWYGVAGAGPLYVYTDAQGQAV
                     LTDHLEQVPAEFRGRVRTMATAEAPPTAAPAPVAAATVSQSPSASGVVEHLLNVIAAK
                     VGSRTIKGLTAHQTAVIILAGVCWLALLVPIFMSSNPGIRLLCKFLLVLVGVAALYQV
                     AISGMPSFEAVTGSPQQGSEQAMENVLGKMRSQTEQSYLAQDARTARQLEQIEPPTQ"
     gene            complement(5478..6257)
                     /locus_tag="PK224_18025"
     CDS             complement(5478..6257)
                     /locus_tag="PK224_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17700.1"
                     /translation="MAHQTVSRLFILCLFCITIVLSRLDPLWAGPYFEDGYLGLTQTE
                     LHDKLGLPQAVRDRKSALRVFTYYPITDWEQYFKKVASPENGEDVYTFKRDGIDVRYS
                     FSYTFDPNDHSDQRPLFVRLVDIEFMPPVALSKVPQLVPEFKPSEDANAPVFRSNIML
                     LFFSGNPSPEARFLVREKGKEALDWSLTFQMFALQGLPDPLTSKAPIDRLEISTQSLP
                     LVKLRQRNTHEPVLNPYSKAFAQQPPPPPTVKKIPVPKYAD"
     gene            6445..10128
                     /gene="metH"
                     /locus_tag="PK224_18030"
     CDS             6445..10128
                     /gene="metH"
                     /locus_tag="PK224_18030"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRB17701.1"
                     /translation="MPVHEVRDLLRERILILDGAMGTMIQRYKLDEAAFRGERFRNWT
                     KDLKGHNDLLNVTRPDVIEAIHRQYLEAGADIIETNTFNSQSVSLADYGMDDLGYELS
                     KAGAECAKRAVAQVVAAQPTRRCFVAGAIGPTTKTSSVSTDSNDAAARGTTYPELVQA
                     YGEQVRGLLDGGVDLLLVETIFDTLNAKAAFFAIQQLFAEGARQVPIMASVTFIQAGS
                     NRGFSGQTVEGFWNSISHVPLLSVGMNCALGPKEMRPLIEELSQIAPIFVSSHPNAGL
                     PNPLLPTGFPETPDSLAPQLREWAQNGWLNIVGGCCGTTPDHIRSIATAVKGVTPRQP
                     SKVEPYLRLSGLEALTVRPESNFVNVGERTNITGSPAFSKLILAGDYDKALTVARQQV
                     EGGAQVIDINMDEGMLDSKAAMQKFLRLLAAESDIARVPIMVDSSKWEVIEEGLRNMQ
                     GKGIVNSISLKEGEAKFLEQARLIRRYGAAIVVMAFDERGQADSVARRIEICERSYRL
                     LTEQVGVPPQDIIFDPNILTVATGLEEHNNYAVDFIDATRWIKQHLPLAKVSGGVSNI
                     SFSFRGNNVVREAMHAAFLYHAIQAGLDMGIVNAGQLAVYEEIPKDLLTLVEDVLLNR
                     RPDATERLVVFADTVKQKGKTAVKDDEWRTRPVEERLAHALVKGLTDYIDQDVEEARQ
                     KSSRPLDVIEGPLMAGMNIVGDLFGSGKMFLPQVVKSARVMKKAVAYLMPFMEAEKLR
                     LGTSRANGKVLLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVSCEKILAAAREHQ
                     VDMVGLSGLITPSLDEMVHVAKEMTRQGFTVPLLIGGATTSKAHTAVKIAPSYGHATV
                     HVLDASRAVGVVGSLINEEQRESFSAGVRADYDRIRQAHQDRGPKTLRSLEAARKHKL
                     STDWAAADIPTPSFTGVRQIDRMPLRELVPYIDWSPFFHTWELRGRYPAILDDATVGP
                     KAKELLADAQALLEEIIQGELLTARGVYGFFPANSAGDDIHLYRDPDRREPLATFHTL
                     RQQMEKPTDQFNLALADYVAPKESGRADYVGAFVVTAGIGVPALCAKYEKDHDDYNSI
                     MVKALADRLAEAFAEWLHKQVRLEWGYGKTEDLTNEDMIRERYRGIRPAPGYPACPDH
                     TEKRTLFDVLNAEAQAGVTLTESYAMQPAAAVSGLYFAHPEAKYFAVGKINRDQVEDY
                     ATRKNLPVGEVERWLAPNLNYDT"
     gene            complement(10233..10730)
                     /locus_tag="PK224_18035"
     CDS             complement(10233..10730)
                     /locus_tag="PK224_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17702.1"
                     /translation="MTRTSRNWIAYLCESLASSGMMPTWEAATYLTDNLFGDKPNPRL
                     LRTACPYEDTTHLLHRLYQPLVEAATRDIPILAAAMIHVFTDISLTGKSAVLVVYFPG
                     QNQPHQLLLLDAAKAWELVFENSEDFNTWAEERYHWIKSSLTSVMAGVSISPAIPAPF
                     EAVKN"
     gene            complement(10727..11905)
                     /locus_tag="PK224_18040"
     CDS             complement(10727..11905)
                     /locus_tag="PK224_18040"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17703.1"
                     /translation="MSIPSVTEVTTQQAILENRNILIVDDEEPIRRLLGYLLEPHGYK
                     VALAGEAREARQRMESGSYALVLCDVNMPGESGMDLIRHILTQYPSTAVIMITGLDSP
                     VLANAALDMGAFGYVIKPFEANEVLINVANALRRRKLEIENAMHRENLEEVVRTRTIA
                     LQQALEWLERSEKELRLSREETIQRLAIAAEYRDSSTAQHIQRMSHYCELLARRYGLS
                     PERCDLIRTASPMHDIGKIGTPDHVLLKPGKFTPEEFKVITQHTEIGYRILAGSDSEL
                     LKVAALIAWTHHERFDGTGYPRGLKGETIPLEGRITAIADNFDALTTQRVYKPAYDFD
                     HAKELMLKERGKHFDPELLDIFFDSMEEITRIYDQFADPTWLSTSRHRAITQDQGEVA
                     "
     gene            12377..13120
                     /gene="rph"
                     /locus_tag="PK224_18045"
     CDS             12377..13120
                     /gene="rph"
                     /locus_tag="PK224_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRB17704.1"
                     /translation="MASGAGLGRIDGRRRDQIRPVKVTRNFTKHAEGSVLIEMGDTKV
                     ICTASIEEKVPPFLKGKGAGWVTAEYAMLPRATHDRSPRESVKGKQGGRTLEIQRLVG
                     RALRAVIDTGRLGERTIWIDCDVIQADGGTRTASITGSFIALADAVGVLKKKDLIKVN
                     PLTDYLAAISIGKVGGQVMVDLAYEEDSHAEVDLNLVMTGAGQYVEVQGTAEKTPFNK
                     KDMDEFLDLGWGAIRELVDLQKSLIGSLS"
     gene            13123..13737
                     /locus_tag="PK224_18050"
     CDS             13123..13737
                     /locus_tag="PK224_18050"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HRB17705.1"
                     /translation="MQIVLATRNQHKKQELVALLGGMGITIRTLDDFPDAPDVVEDGE
                     TCEANAMKKAVEIARYTGLTAVADDTGLEVDALGGRPGAFAARYAGEQASYEDNWRKL
                     LQELRGVPAENRGARFVTVAAIAFPAGKTFSTKGVLEGLIAEEPAGSRGFGYDPVFFL
                     PEYRQTLAQLPPDVKNRISHRARAFTQARTLLEQMTAEQNSVGA"
     gene            13728..13804
                     /locus_tag="PK224_18055"
     tRNA            13728..13804
                     /locus_tag="PK224_18055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13762..13764,aa:Pro,seq:tgg)
BASE COUNT         2731 a         3826 c         4442 g         2850 t
ORIGIN      
        1 catccggatg cgttggcaag cctccaatcc atcgatctcg ggcatttcga tatccatcaa
       61 caccaagtcg attccgctgg tctgctttcc acgtttcccg acccccagga gttgcagccc
      121 ttcccgggcg gtgccgaccg agaggagcgg gccgaacccc gcactgtgga gaatggtgaa
      181 gagcagatcc cgctcttcct ggcagtcgtc gatgatcaag atgctcatgt ttcctctgtt
      241 tctccgaaga gtctcctcaa atcgagtcag gcggcttcat cgactcctag gaggatagtc
      301 gtcagtctat cgatttcacg gcgaatcgcc tctcgatcgc gagattgggc tgcctgttca
      361 aggcggtgac cgaccgcccc gatatcagga aatccgaacg agcctgcaag gcctttcatg
      421 cggtgcccag tggtgcgaat cgcttcgtag tctcccttac ccagggcgtc ctgcatcatg
      481 gcgacatctt tacgccgatt ggcgagaaat gcaggtcggc ggcgccggat ctcgtcgtcg
      541 atgcgctccg tcaggtctgg tgatgccgca ggttgcgccg gtgtggtgcc cgttccagga
      601 aaagaccggc gatatttgcg aagagtactc aacaatcgtg cctttgtgat tggtttcgtc
      661 aggaaatccg tgcagcccgc ctcgaggctg cgtgcctgac tctcaactcc gctgctcgcc
      721 gtcacagcca cgattgggat ggggggatgt ttgtgggagg cttcccaggc gcgcatcgcg
      781 cgggtggcgg tgaatccgtc caacccgggc aggtgcaggt cgatgaggac gaagtcgaat
      841 aggccggtct ggaaagtggc gacggcttgc tcgccatccg gggcgatctc caggtcgtac
      901 ggcaagttcc ggaaatagga ccgcatcagt gtatgcgact caattgaatc gtcgaccagc
      961 aagacgcggg ttttctgttc tgccggttcc gcgggcacgc gcggcaggac cggttccgtg
     1021 gcctgagatg ccggtcctag tcgccgggga tgatccgttc ctgggaacgc gggtcggctg
     1081 acctcctgca ggccgcaagc ggcaggctgt cgagcgagct gataggcgat gatccataga
     1141 ccaaggatcc caatcgtgcg actcacgagc gtattcgtcg cttcgtcggt cgggagggga
     1201 gtgaggcatc cggccgccat caacagcgac cagcctccca cggcaaatag gaacatgtgt
     1261 cggggataga gccgggaggc cggaaccggc agcaggagat acgcgaccca gctggccagt
     1321 ctgaattcag aaatcagttc agccaggaag atcaggcccg tcagcaggac aaggaggccg
     1381 gtcgtcgtaa atcgggcggt aaggcgtgtc atggtgcggt gctcgaacgt tcagcgccca
     1441 acgacgcgga tgatgcgtcg ggctcacctg cagtatagcg gcgattgcgt agggcaccta
     1501 ggatctcctg aaattcgagc aggcggaatg gggcagcaca gaggtactca gcatgcgaac
     1561 tgaagtcgcg acgggagggg cctcggtcag taagcgtccc gggcacaacg gaacccggtt
     1621 ccagaagggc ggctgtccat tgtcccccag tcgcgatcag tcgtgcgtaa gctgatcgcc
     1681 ggtttaagcc aggatccgcc gcgcatggcc ttgatggccc cgcgttccgg accctggggg
     1741 ttggtaagcg tcgccgtgcg gtaatcgttg gggttgtacc agtcctgcac ccattcggcg
     1801 gcattgcccg ccatgtccag cgctccgtag gggctggcgc cggccgggaa actgcccacc
     1861 ggcaaggtca ggatctcgcc cttgaggccc ctttctttgg aggtggctgc gccgacaccc
     1921 ttgacccaga acgcgtccca ttccgtgctg tcggcgaact gcaccgtctg acgggcccag
     1981 tagctcgcgc tgttgccgtt ctcgaagttc cactcgttgc cccagggata ggttcgcccg
     2041 tctgttcccc gtgccgcctt ttcccattcc gcttcagtcg ggagccgttt gttcgcccag
     2101 cgacagaagg ccacggcatc aagccaactg acattgacca cgggatgttg ttcgatgccg
     2161 ggcaggggcg cattgtgctc ccacaacgtc aatgccggag cggagttggc tggtgcctgg
     2221 tatccggtgg cctgcacgaa ttcctgatag gctgcgttgg tgacttcgtg ccgatcgatc
     2281 cagaacgccc cgaggtagat gagccgtagc ggacgttcat ccggaagccc gccgctctct
     2341 accgaggccc ccatcgtaaa ctcacctgcc ggaaccatga ccatgtcgtc aacccgaaga
     2401 gccgcggaag ctgatggttg atagccgacg gccggaaaga gacagagtgt cagcagtagc
     2461 aaccgtgtca gtctcatccg ggttaggctt tcttcagctt acaggctttg gcgacgttgt
     2521 cacggtaggc cgtccacaag gcgaggcctt tggtgacgca tacgagcaca gcgttttgtt
     2581 ggcttccggt ttcggcgtag ttgacgacca tcgtgtacgc atcctgtcga tgacccgcct
     2641 caaccttctg gtgggcgcga atcaaatcca tgtgctgccg gccgatccca tggtgacgaa
     2701 taagaggatg ggcatcgata taagcttcaa tctcggtctc ggttttcggc ttggagggcg
     2761 cctggatctc ctgccggtcc ttcacgctgc cctcgacaaa cacggcgagg gcggccgccc
     2821 cgaccaccca gtcacgactg gccgtgacct tctccagcca ggctcgatac cgcgcgctgg
     2881 cagggaggag tttcgtcctg cgaaacgtct cgctgtcgaa gcccaggccg cgcatcattt
     2941 cgttaaagag ttcgggatgg gatcggccga aggagagccc gccggtatct tcctcataaa
     3001 tattctccgc caacatgcgc cgcacatcgg aaggcggatt ctgcccgtgc acgcgggcca
     3061 gcagaacagg gaagtcgcga acgtagacca ggaactcctg ttgatagtgg agttttagct
     3121 gggctttcgt gatgccgggc cccatgatga tcggccaggc ccagtggtgt ttgcggtcca
     3181 tgatcgccaa caaccgctcc agaaattgcg tgcgggtcaa tctcttgtgt gccatgcctt
     3241 gccttccttc tttctcacta gctacaatca gccaccgatc agatgaagcc tatcacgatt
     3301 caagattccg accagattct caacttcctc gccgaagtgg ccctgcacgg caaggggttt
     3361 accaccgact gcctgctgga ttatgtcctg gacgaaggat ttaccgaacc gatctatctg
     3421 aacgcctccg gtgaggatcc ggatgccttc tataaggacc agcctcaggc ctgggcgatc
     3481 taccaagtcc gcgagtggaa gcgagtgttg actgtgtcgg ggggcgccgg gaaggaacgg
     3541 cgcgtgcaga tcactgaaac cccgtaagtc gtccgtgtcg taccgccggt gccagccaac
     3601 cggtggatgg cttctcgcct cgctcgcatg tggtatggcc gagctgatgg aaggtgcagt
     3661 actcactcat cagtcagtgt attgaccaac cgacagaatt gcaacgaatc gcgcactgtc
     3721 agccgcccgg cctgccggtt ccagacaagc ctgatcggaa tgtggcttcg aactgccgac
     3781 cgggaggccg accttcatgc caagacacca ccctgaaaat tccacgaagc tggtcaccct
     3841 gtgtgagtcg ggtgatgtcg gtgagttggc gttgatcaag agcctgctcg acggcaatgg
     3901 gattacctac acggtgcagc atgagcacgt cggggcgttg taccccgggg tcgcgctgtt
     3961 gggcagccgg gtgatggtgg aggagcggga caagcagcgg gccgaggtgc tgctcagccg
     4021 tctggccatg cacattcgtg aaacgaccgg tgatgtggac ggatgacgtc ttgagcagcg
     4081 ggctaccggc cggtcaacgg ccgcctacgg atgacccttc ggtttgaccg gctcaggtgt
     4141 ggtgggaatg ggcaggcgca catccgatcc cgaaaaatgc ccgccgatca gcttgccttc
     4201 ttcgaaatac aagtagcggt gcttcacgac cgccggactt tcccagtctt cgaaaatcca
     4261 ttcttcgcgc cgcagcccgt cgctggtgtg tgtcacattc atcgtattcg gggagcccca
     4321 ggagtcgagg acctgtttac ggtccatgcc gaccatcact cggtggcttt cgatgtgctt
     4381 cgagaagtcc ggatccagcc agcccggcac gaaacgaaac gcgatcaggg cgaccacgaa
     4441 taaccccaga aacgtatgga taatgatccg gaccggccgc tggcggatga agggtgtcgg
     4501 ttccaccgcc ggggggaggg ccgaaacggg agttgcagag gtcgtctgat cggtcacaat
     4561 ggtccttccg tcatcgaatg ggcaagaaga gtgctggtac ttctgcatgc taaacaatcg
     4621 tttttcgctc cgtcaaggga gggggagggc atcagagacg ggtttcactc ccttgagccg
     4681 gctgttcgac ctgctatgct tgaagcagga ccgcggaggg cgacgcatgg ggtgctggcg
     4741 ttattcgatc aaccgatgtt ggattgggct gatcatgctg gggatttgcc tgacatggta
     4801 cggcgtggcg ggggcgggac cgctctatgt ctacaccgat gcccagggac aggctgtgct
     4861 gaccgatcac ctcgagcagg taccggcaga gtttcgcggg cgtgtgcgaa cgatggccac
     4921 tgcggaagct ccgccgaccg ctgctcccgc gcctgtggct gctgcaaccg tcagccagtc
     4981 accgtctgct tccggggtgg tggagcacct cctcaacgtc atcgcggcaa aagtcgggtc
     5041 ccgcacgatc aagggcctca cggcccatca aaccgccgtc attatcctgg ccggcgtctg
     5101 ctggctggca ctgctggtgc caatatttat gagttccaac cccggcatcc ggctgctctg
     5161 caagtttctc ctggtgctgg tcggcgtcgc ggctctctat caggtggcga tctcaggcat
     5221 gccttccttc gaggcggtga ccggctcgcc tcagcaggga tcggaacagg ccatggagaa
     5281 tgtgctgggg aaaatgagaa gccagaccga gcaaagttat ctcgcgcaag acgcccgcac
     5341 ggcccgtcaa ttggagcaga tcgaaccgcc tacccagtaa cgatcccggt gggatgagac
     5401 agacttgcag caaggcgtgt ggctttcgcc cactctgtag cttcgagagg gcagggagca
     5461 ggaccgtccg gaacctctca gtcggcgtat ttgggcaccg gaatcttttt tacggtcggc
     5521 gggggaggcg gctgttgggc gaaggccttg gagtaagggt tcaggactgg ctcgtgggtg
     5581 ttgcgttggc ggagcttgac cagcggtaaa ctctgcgtgc tgatctccag gcggtcgatc
     5641 ggggccttcg aggtcagcgg atcgggtagt ccctgcaggg cgaacatttg aaaggtgagt
     5701 gaccagtcca gggcctcctt ccccttttcc cgcacaagaa atcgcgcctc cggcgagggg
     5761 tttccggaga aaaacaacag catgatgttc gaacggaaga ctggcgcgtt ggcgtcttcg
     5821 gagggcttga actccgggac gagttgggga accttgctca acgctaccgg cggcatgaat
     5881 tcgatatcta ccaggcgcac gaagagcggc cgttggtcgc tgtggtcgtt cggatcgaag
     5941 gtgtagctga acgaatagcg cacgtcgatg ccgtcacgtt tgaaggtgta cacatcctct
     6001 ccgttttctg gtgaggccac cttcttgaag tactgctccc aatcggtgat cggataatac
     6061 gtgaagacac gcagcgcaga tttgcggtcc cggacggcct gcggcagacc gagcttgtcg
     6121 tgcagttccg tttgcgtgag cccgaggtag ccgtcctcga agtaggggcc tgcccacaga
     6181 ggatcgagtc tcgacaagac gatagtgata cagaacaagc agaggatgaa caggcgggag
     6241 acagtctgat gcgccatagg attgtctcag gctcgggcag acaacggtgg gcatcgtatc
     6301 ggccggggta gtcaatgtca aggcgaagag cctgcaatag cttgctgaaa aaagtccgtt
     6361 cccgatatga tggccgtcca attgccagtc atcgacgcag acggcgcacg cctgacgtcc
     6421 ggtggcagaa agacataggt agtcatgccg gtacatgaag ttcgcgacct cctgcgagag
     6481 cgcatcctga tcctggatgg tgccatgggt accatgatcc agcggtataa gctggacgaa
     6541 gccgcgtttc gtggggagcg ttttcgaaat tggaccaaag atctcaaggg acacaacgat
     6601 cttctgaatg tcacccggcc ggatgtcatt gaggcgattc accggcagta ccttgaagct
     6661 ggcgcggata tcatcgaaac caatacgttt aactcgcagt ctgtgtcgct ggctgattac
     6721 ggcatggatg acctgggata tgaattgtcg aaagccggcg ccgaatgcgc gaagcgggcc
     6781 gtggcacagg tcgttgccgc gcagccgact cgccgctgct ttgtggccgg tgcgatcggt
     6841 cccaccacca agacctcttc ggtctcgacc gattcgaacg atgcggcggc gcgtggaacg
     6901 acctatcctg aattggtgca ggcctacggg gagcaggtgc gcggcctgtt ggacggcggc
     6961 gtcgatctgc tcctggtcga gacgattttc gatacgctca acgccaaggc cgcatttttc
     7021 gcgatccagc aactgtttgc ggaaggggcg cggcaggtgc cgatcatggc ctcggtcacc
     7081 ttcattcagg ccgggagcaa ccgtgggttt tcgggtcaga cggtcgaagg gttttggaat
     7141 tccatctcgc atgtgccgtt gctcagcgtg ggcatgaact gcgcattggg gccgaaagaa
     7201 atgcggccgc tgatcgaaga gctctcgcag atcgcgccga tttttgtcag cagccatccg
     7261 aatgccggat tgcccaatcc cttactgccg acaggatttc cggaaacgcc ggattcgttg
     7321 gcaccacagc tgcgcgagtg ggcgcagaac ggctggctca atatcgtggg cggctgctgc
     7381 gggacgacgc ccgaccatat ccgttcgatc gccacggctg tgaaaggtgt gacgccacgg
     7441 cagccctcca aggtcgagcc ctacttgcgg ctgagcggcc tggaggcctt gacggtgcgg
     7501 cctgagtcga atttcgtcaa tgtcggggag cgcaccaata tcaccggctc accggccttt
     7561 tccaagctga ttctggccgg cgattatgac aaggcgctga ccgtcgcgcg tcagcaggtg
     7621 gaaggcgggg cgcaggtcat cgacattaat atggacgaag gcatgctgga ctccaaagcg
     7681 gccatgcaga agtttctgcg gctgctggcg gcggaatcgg acatcgcccg tgtgccgatc
     7741 atggtggaca gttccaagtg ggaggtcatc gaagagggcc ttcgcaacat gcaggggaag
     7801 ggcatcgtca attccatcag tctgaaggaa ggcgaggcga agtttctcga acaggcgcgg
     7861 ctcatccgtc ggtacggcgc ggcgatcgtg gtcatggcgt tcgatgaacg ggggcaggct
     7921 gactcggttg cacggcggat cgagatctgt gagcggtcct atcgtctgct gacggaacag
     7981 gtcggggtgc cgccgcagga tattattttc gatccgaata ttctcacggt cgcgaccgga
     8041 ctcgaagagc acaacaacta cgcggtcgat tttatcgatg cgacacggtg gatcaagcag
     8101 cacttgccgc tggcgaaggt cagcggcggc gtcagcaata tttcgttctc gttccgcggc
     8161 aacaacgtcg tgcgcgaggc gatgcatgct gcgttcctct accatgccat acaggccggc
     8221 ctcgacatgg gcatcgtcaa tgcggggcag ctggccgtct acgaggagat ccccaaagat
     8281 ctgctgacgc tggtcgaaga cgtgctgctg aatcgccggc cggatgccac ggagcggctg
     8341 gtggtctttg ccgacacggt caaacagaag ggcaagacgg cggtcaagga tgatgagtgg
     8401 cggacaagac cggtggagga acggttggcc catgcgctgg tgaaaggact gaccgactac
     8461 atcgatcagg acgtggagga ggcgaggcag aagtcctccc ggccgctcga cgtgatcgag
     8521 gggccgctga tggcgggcat gaacattgtc ggcgatctct tcgggtcggg aaaaatgttc
     8581 ctgccgcaag tggtgaagag cgcccgagtc atgaagaaag cggtggcgta tctcatgccg
     8641 tttatggaag ccgaaaagtt acggttgggc acgtcccgcg cgaacggcaa ggtcttgctc
     8701 gcgaccgtca agggcgatgt gcacgatatc ggcaagaaca tcgtaggtgt ggtgttgggc
     8761 tgcaacaact atgaggtgat cgaccttggc gtgatggtgt cttgcgaaaa gattctggcg
     8821 gccgcgcgtg agcatcaggt ggatatggtc gggctgagcg gcctgatcac tccatcgttg
     8881 gatgaaatgg tgcatgtggc caaagagatg acgcgccagg ggtttacggt gccattgctc
     8941 atcggcggcg ccacgaccag caaggcccac acggccgtga aaattgcccc ttcatacggc
     9001 catgcgacgg tgcatgtgct cgacgcctcc cgcgccgtcg gtgtcgtggg cagtctgatc
     9061 aatgaagagc aacgggagag cttttctgcc ggcgtacgtg ccgactatga ccgcatccgg
     9121 caggcgcatc aggatcgagg gccgaaaacc ctgcgtagcc tggaagcagc ccggaagcac
     9181 aagctgtcga ctgattgggc cgctgccgat atccccacgc cttcattcac cggcgtgcgt
     9241 cagatcgatc ggatgccgtt gcgtgagttg gtgccctata tcgattggtc gccgtttttt
     9301 catacgtggg aattgcgcgg ccggtatccg gctatcctgg acgatgccac ggtgggtccg
     9361 aaggccaaag aactcttggc ggatgcacag gcgttactgg aggagatcat ccagggtgag
     9421 ttgctgacgg ctcgcggtgt ctacgggttc tttcccgcca atagcgcggg tgacgacatt
     9481 catctctatc gggatccgga tcgacgtgag ccgctggcca ctttccacac gctccggcaa
     9541 cagatggaga agccgaccga ccaattcaac ctggcgcttg cggattatgt cgcgcccaag
     9601 gagtccgggc gtgccgacta tgtgggcgcg tttgtggtga cggccggcat cggcgtgccg
     9661 gcgctctgcg cgaagtatga gaaggatcat gacgactaca attccatcat ggtcaaggcc
     9721 ttggcggatc ggctggcgga ggcctttgcc gaatggctgc acaagcaggt gcggctggaa
     9781 tggggatatg ggaagacgga ggacctcacg aacgaagaca tgattcgcga gcggtatcgg
     9841 gggatccggc cggctcccgg ttatccggcc tgtccggatc acacggagaa gcggacgctc
     9901 ttcgatgtat tgaatgccga ggctcaagcg ggggtgacgc tgaccgaatc ctacgccatg
     9961 cagccggcgg ctgcggtcag cggcttgtat tttgcgcatc cggaggccaa gtattttgcc
    10021 gtgggcaaga tcaatcgcga tcaggtggag gattacgcga cccgcaagaa tctaccggtc
    10081 ggcgaagtag agcggtggct ggctccaaat ctgaactacg atacataacg tgaagcgttg
    10141 atcgtgaagc gtatctcgtt tcggtcatac gcgcactcct tccgctgttg cggaagggac
    10201 gaacgacgtt tcacgaacga cgaggaacgg catcagtttt tgacggcttc gaagggcgcg
    10261 gggatggcag gggagatgga tacgcctgcc atgacactcg tgagcgagga tttaatccag
    10321 tgataccgtt cctccgccca ggtattgaag tcttctgaat tctcaaatac cagttcccag
    10381 gctttggcgg catccagcag taggagctgg tgcggctgat tctgccccgg gaaatacacc
    10441 accaacactg ccgattttcc ggtgaggctg atatccgtga agacgtggat catggctgct
    10501 gcgagaatcg ggatatcgcg cgtggcggcc tcgaccagcg gttgatacaa ccggtgcagc
    10561 aggtgcgtcg tatcctcata ggggcaggcg gttcgcaaga gtcggggatt gggcttgtcg
    10621 ccgaacaggt tgtcggtcag gtaggtggcg gcttcccagg tcggcatcat gccggaggag
    10681 gcgaggctct cgcacagata cgcgatccaa ttgcgtgatg tgcgggtcat gcgacttctc
    10741 cctggtcctg cgtgatggcg cggtgacggg aggtggacaa ccaggtcgga tcggcaaatt
    10801 gatcgtaaat gcgtgtgatc tcctccatcg agtcgaagaa gatatccagc agttccggat
    10861 cgaagtgctt gccccgctcc ttaagcatca gttccttcgc gtgatcgaaa tcataggccg
    10921 gcttgtagac acgctgcgtc gtcagggcgt cgaagttatc ggcgatggcg gtgatccggc
    10981 cttcaagcgg aatcgtctcg cctttgagtc cgcgcgggta accggtgccg tcgaagcgtt
    11041 cgtggtgcgt ccaggcgatg agcgcggcga ctttcaagag ttccgaatca gacccggcca
    11101 gaatccggta cccgatttcc gtatgctggg tgatcacctt gaattcttcg ggtgtgaact
    11161 tgcctggctt gagcagcaca tggtccgggg tgccgatctt gccgatgtcg tgcatcggac
    11221 tggcggtgcg gatgagatcg catcgctcgg gtgaaagccc gtagcgacgg gccagcagtt
    11281 cgcagtagtg gctcattcgt tgaatatgct gcgcggtcga actgtcgcgg tattcggcgg
    11341 cgatcgcgag ccgttgaatg gtttcttcgc gggagaggcg cagttccttc tcgctccgct
    11401 ccagccactc aagtgcctgc tgaagggcaa tcgtcctggt ccggacgact tcttccagat
    11461 tctcccggtg catggcgttt tcaatttcca acttgcgtcg tcgcaacgcg ttggccacgt
    11521 tgatcaggac ttcgttcgct tcgaacggct tgatgacgta accgaaggcg cccatatcca
    11581 aggccgcatt cgcgaggacg gggctgtcga ggcccgtaat catgatgacg gcggtgctgg
    11641 ggtattgcgt caggatgtgg cgaatgagat ccatgccgga ttcgcccggc atgttcacgt
    11701 cacacagcac caacgcatag gaaccgctct ccatccgttg acgggcttcg cgcgcttctc
    11761 ccgccagcgc caccttgtat ccgtggggtt cgagaaggta gcccaataat cgacggatag
    11821 gctcttcatc gtccacaatc aggatgttgc ggttttcgag gattgcttgc tgggtggtga
    11881 cttcggtgac gctcggaatg ctcatatggt tcgccgggct aggttaatcg tggtgtcgat
    11941 cagcaataga gtcagtatcg gtcgttgggc gtaaagtctg aagtgcgaga tggattgagg
    12001 ggaaaagcac cttttatgcc actcaagccg cggtgcgacc aactgtcccg tgtcgcccgc
    12061 ccattgctgc atcaatgctg ggaactctgt tctatcggga caagatccga cgagtggcag
    12121 tttccggccg ctctgcccgg cccggggctg tgatcgtcaa ctctgacgct cgtccgttgg
    12181 tctttgagca ggcggcgttc ccgagggaac agcaacctgc accatcacgc tggattatac
    12241 agggggcttt cccgattgcc agccgaaacg cagtttgaga aacgagcacc ggtcgcgccg
    12301 ctggatttgc tctttgaagc cctgttcact ataatccgct cctttagatt tgctgtgcag
    12361 gaaggagcgc gactcaatgg cgagcggagc agggctaggt cgaatcgatg gccgtcggcg
    12421 ggatcaaatc cgcccggtca aggtgacgcg caattttacg aaacatgccg aaggctccgt
    12481 cttgatcgaa atgggggaca ccaaggtcat ttgcacggcg tcgattgaag aaaaagttcc
    12541 gccgttttta aaaggtaagg gtgccggctg ggtgacagcc gaatatgcca tgctgccccg
    12601 ggccacgcac gatcgttcgc cccgggaatc ggtcaagggt aagcagggcg gccgcacgtt
    12661 ggagattcag cggttggtcg gacgtgcgct ccgtgccgtg atcgatacgg gccgtttggg
    12721 cgaacggacc atctggatcg attgtgatgt gattcaagcg gatggtggaa ctcgcacggc
    12781 ctccattacc ggctccttta tcgccctcgc ggatgccgtg ggtgtgctga agaaaaagga
    12841 cttgatcaag gttaatccgt tgaccgatta cctggcggcc atcagtatcg gcaaggtcgg
    12901 gggacaggtc atggtggacc tggcctacga agaagactcg catgccgagg tggatttgaa
    12961 tctggtgatg accggcgccg ggcagtacgt cgaagtgcag gggacggctg aaaagacgcc
    13021 gttcaataag aaagatatgg atgagtttct cgatctcggc tggggcgcga tccgtgaatt
    13081 ggtcgatttg caaaagtcgt tgatcggctc gctgagttaa ccatgcagat cgtgctggcg
    13141 accaggaatc agcataagaa gcaggagctg gttgccctat taggcggtat gggtatcacg
    13201 atccgcacac tggacgattt tcccgacgcg ccggacgtgg tggaggacgg cgagacctgt
    13261 gaagccaacg cgatgaaaaa ggctgttgag atcgcccggt atacggggct tacggcggta
    13321 gcagatgata ccggattgga ggtcgatgcc cttggcgggc gtcccggtgc ctttgccgcg
    13381 aggtatgccg gagaacaggc cagctacgaa gacaattggc ggaagttatt gcaggaactt
    13441 cgcggtgttc cggcagaaaa tcgaggtgcc cgctttgtga ccgtggcggc cattgcgttt
    13501 cctgccggca agaccttctc gaccaaagga gtgttagagg gactcattgc cgaagagccg
    13561 gcgggctccc gtggtttcgg gtatgatccg gtctttttcc tgccggagta tcgtcagact
    13621 ctggcccagc tgccgccgga tgtgaaaaat cgaatcagcc atcgggcccg tgcttttacc
    13681 caggccagga ctctgctcga gcagatgacg gcggaacaga actcagtcgg ggcgtagcgc
    13741 agctcggtag cgcgcctggt ttgggaccag gatgtcggag gttcaaatcc tctcgccccg
    13801 accaaacaaa gcttgctcga ccccgaattt caaatgtgcg cggggatat
//
LOCUS       DAORRQ010000082        13212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_231_length_13212_cov_73.941932, whole genome shotgun sequence.
ACCESSION   DAORRQ010000082 DAORRQ010000000
VERSION     DAORRQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231_length_13212_cov_73.941932"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..729)
                     /locus_tag="PK224_18060"
     CDS             complement(187..729)
                     /locus_tag="PK224_18060"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRB17706.1"
                     /translation="MKFCSECGAGLSKKIPPGDNLPRFVCDTCQVVHYINPKIVAGCI
                     PEWEGKILLCRRAIEPRTGHWTFPAGFMEIGESTEQAAIRETFEEAHADVEITSLYAV
                     LSLPRISQVHMVFRGSMRTPEFKPGTESLDVQLFSLEDIPWDDLAFPVITEALERYVA
                     DVARGTFSMHFGSVFPRMKS"
     gene            764..1933
                     /locus_tag="PK224_18065"
     CDS             764..1933
                     /locus_tag="PK224_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRB17707.1"
                     /translation="MDSTNHAFFAPCPRGLEAVLADELTDLGAADVKPTAGGVAFHGP
                     LALCYRVNLQSRIASRILLRIAEGSYRDEHDVYDAANAIRWQDWFTPQQRIKVKVSAH
                     HCPLKSLDFVTLRVKDAVCDRFQFARGKRPTVDTHAPDILIAVYLDASTCTFYVDTSG
                     EPLFKRGWRRSAGEAPIRENLAAGILRLSKWTADTVLYDPMCGSGTFVIEAALMARRV
                     APGIGRHFAFEKLLSFDAQRLNDLRTELKAMEVPIKPGLIHAADDSAHAMTSIKANLT
                     IAGSGDAVTLQQGDVLQLPAPAESGLLVTNPPYGHRMGEAESLRTFYPQFGDHLKKHF
                     CGWTVQIFTADLKLPGQLRLAPSRRVPLFNGAIECRLFEFRMVAGSLRKKRANGE"
     gene            2335..3861
                     /locus_tag="PK224_18070"
     CDS             2335..3861
                     /locus_tag="PK224_18070"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRB17708.1"
                     /translation="MNASHAPLEELQPEQDLSLSRLIVVSNREPYEHRLVKNHLIWER
                     TSGGLISALDPVMRRLGGTWIAWGSGKADREVVDQDMIVEVPPDAPTYHLRRVWLEPT
                     EIKGGYQGYANQVLWPLCHLTLDRVAYRKAFWHAYQAMNARFAAAVFGELRGKSGFVW
                     VHDFHLALVPGLIKAALPAQPVAVFWHTPWPGPDAFRILPERRELLESLLASDLLMFQ
                     THNFLHCFAECVKEFLGVEVRSDDHHIEYNGHTTRLVARPISVSFQAWSERARTSQVT
                     RAMDVLRNLHVFQPEVRIGLGVDRLDYTKGLLKRFWAIDAFFEQYPQYRGRFTFVQVA
                     VPTRGDVEAYRSYRELIRETVNDINMRYGSISNAGSPQASRWRPIEFREGRIGMDTLA
                     AYYRMADLALVSSVYDGMNLVAKEYVASQVDEKGVLLVSHMAGAAEELTDALVINPYD
                     LEGVADAIRQAIEMPLEERRERMHRMRTYLETHDIRAWSDECLRDAGILPPDDSPTLE
                     "
     gene            4027..6009
                     /locus_tag="PK224_18075"
     CDS             4027..6009
                     /locus_tag="PK224_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17709.1"
                     /translation="MSLCLAFGGCAQIAHLFAVTQPSDQPVDRQQQAIVTRLKALVEP
                     DLQDNLNTALPAVRKSLADQTNKIAKFKRNLVLRTLADPWDGLAAAEQAGLLAATTAE
                     SGVEGLPAVIDILEGGMDRTGASFAPLTFPVTLATEDLLTFLTDVLEQAYQDREHALR
                     HLSPQDRDFLFTQARPLVEHFTPQVAPPAQLSEAEAAARYATLLMQQVDYASLISAAQ
                     RLARLGNRKFLQQLDIALHNRKPIGQTVPGITGDLLLAQQTSYGLVVVGGHGPNTYDL
                     EKGAALIIDLGGNDTYRGTIGASPNSDLGNSVVIDLSGNDTYQPAPLGLATGRAGIGI
                     VIDHSGDDTYRLAPGSGGVGLAGLGILYDGEGQDIYEGNRFTQGAAFGGFGLLVDRAG
                     DDRYTSFGYALGFGAPLGVGALIDVSGNDFYECGGRYPSAYNETDAPNAHPQDPVFQY
                     DCFGLGTGSGLRVFSKQPAHRALSLAGGWGLFIDLDGRDRYRSANFSQGHGYFFGLGV
                     KLDLNGDDEHQAARYGHGTAAHFGVGLNIDYQGKDRYGSKGPFYNGGAAWDGSVALAV
                     DGGPDSDFYDLPASTGLGMGDLGGWGLFIEEGGADQYAVSRGLGQGAEHSLGAFFDLE
                     GRDDYSSVSPPAKGVRPERLNGKTFIENAGSLFIDR"
     gene            6303..8699
                     /gene="lon"
                     /locus_tag="PK224_18080"
     CDS             6303..8699
                     /gene="lon"
                     /locus_tag="PK224_18080"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRB17710.1"
                     /translation="MNESNAPILTHLPVLPLKRTVLFPGTMMPLTVGRDRSIAAVEAA
                     LKTEDKTLLVVAQRDAQTDQPTLEDLYPIGTKAVIKQTARTPEGHYNILIQGLERFVL
                     LKLDQMDPYLQARVKQLAPPSEQSTEVEALHRAILDIITELPKLIQTPGVHEAVAALG
                     TEEDPVVLAYRIASLLNLTLDGEQQLLAAPTRADLLRGLYAALSREVQILQLRDKITS
                     EAREKLGKTQREYLLREQLKTIQQELGEGNGEEDEVGVLRSKIHEADLPEHVRKETDR
                     ELARLAKTPSASPEHQVIRSYLELVLELPWNKSSEEGLDLAHVRQVLNEDHYGIKEVK
                     ERIVEHLAVLKLNPSAKAPILCLVGPPGVGKTSLGQSIAKSMGRTFERFSLGGLHDEG
                     ELRGHRRTYVGALPGRIIQAVRRAGVNNPVVMLDEVDKLGRDFRGDPAAALLEILDPA
                     QNHTFRDHYLDLPFDLSKVFFITTANTLETLSQPLLDRMEIIRLNGYSEREKREIALR
                     YLWPRRLKEAGLRAEDVNLPEAVLDHIIGRYTRESGVRQLEQMLGRITRKVAVTFADR
                     PADAPASPVDITQPLLDEWLGQERFQPEEARKNLPAGVATGLAWTPTGGDVLYIETTL
                     LPGSHELTLTGQLGDVMQESARAARSYLWSHAESMGLDISRFKRNGVHIHVPSGAIPK
                     DGPSAGITMATALASAYVGKPVRSDTAMTGEISLTGLVLPVGGIKEKVLAAHRAGIRR
                     IILPKANERDLKEVPQEVRDELTVIPVERIEEVLPAAFNQDPASSPERGEPLASATAS
                     "
     gene            8887..9306
                     /gene="msrB"
                     /locus_tag="PK224_18085"
     CDS             8887..9306
                     /gene="msrB"
                     /locus_tag="PK224_18085"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRB17711.1"
                     /translation="MSPKIEIGKIVKVTKTDDEWKTLLSPAAYQVLRHEDTERAFTSP
                     LHENHQAGIYHCAGCDLPAYSSEHKFDSGTGWPSFWQPIDPKVVEKRTDSRFFMTRVE
                     VHCAQCGGHQGHVFDDGPRPTGLRYCINGVSLKFVPA"
     gene            9448..10599
                     /locus_tag="PK224_18090"
     CDS             9448..10599
                     /locus_tag="PK224_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17712.1"
                     /translation="MRRPLLGLFLAGTAVLGCSQSSDVISSNLNACLIVTEAEVELAI
                     GTPVTPGLRQNDRQCLYQAKRNPQETVTVELNPGPDGDTKGLFHDQRSKAGHQPVPGV
                     GDGAFTLRSPIGGIQLTFLKADALVTLSLSSSKQPNAQAAVTNLAKVAATRLGGPLRT
                     GPAASTEPGLTATPSQWAGDWYGCPPVGLMYGKGHLALSEGGTWSLTTAVLMPGTVTA
                     DRGRWQAESYQEILHGTYQVAGNDRFSTTGILSVTWDKLAKNQGPNKFDPLLWQSFAS
                     VPRKVAVKRLPPVEPSLVGTWEGSAKFVDRQEEFVWTITPANVSEFYRATSQSGRIEK
                     EQDRFRLIVPQGKTPPLFVRVLAQDKLELTDNSGTVSQWNRNEKLLSRC"
     gene            10680..11081
                     /locus_tag="PK224_18095"
     CDS             10680..11081
                     /locus_tag="PK224_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17713.1"
                     /translation="MTRWLMVTLLCTAPLLPAVQSQAAPGDTAQTPDSIESFDLPTPD
                     GLRSLYNQTLAALQEYIEIDGSLPQDGETQARVGEFRLKVFPQGKSRSQEHLSAEGSF
                     RLSPDPGQQEFSLRFKSSKRPSTPQPSGDII"
     gene            complement(11379..12128)
                     /locus_tag="PK224_18100"
     CDS             complement(11379..12128)
                     /locus_tag="PK224_18100"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRB17714.1"
                     /translation="MKSAQWFLAAFILSCSLFETHHAAASAAEPASSAAKAPATSPEA
                     TQAGEKTEFYVSVYLQGSRPLNRPVRLQEDPFTNTDVQGGLGGGLKVGIFPAFAGRFV
                     GLEADLSGLDGKVNAPSATTGGVTRSANFRLQSINVMANVLLRYPGETIQPYIGIGAG
                     LSGGFARDLNLQHSTIGTVHENAADGAFAYQFLGGVRANVTERMFLFTEYKYFVANYQ
                     WESEVANGARGPSFTMNYRAHIISGGIGFTF"
     gene            complement(12317..12889)
                     /locus_tag="PK224_18105"
     CDS             complement(12317..12889)
                     /locus_tag="PK224_18105"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRB17715.1"
                     /translation="MNTCRLYSDFNCPFCYAMHERLHALGVMDRISWQGVQHAPHLPV
                     PMAGWAGHLAAELKQEVQMVRRLAPELPIAVPPGKPNTGRAIAAAARALHMDPLRGGE
                     FVRSLYRSFWVDGQDLSDETLLQREAERQGFASAQIVGTEATTVDAILWAWNDQWAEA
                     DHQGVPLLQRPDGTLLVGLMPADVIKGFLS"
BASE COUNT         2709 a         4144 c         3850 g         2509 t
ORIGIN      
        1 cggcagagga attagggctc atcatgccca gcagcaactg aagcctggtg aaacgtaaaa
       61 agtgaaacgt atcgcgtgcg gagatgatca cgaactgcgc gacaggatca ccaacgaacg
      121 tgatgagccc cgcgtcacgg tcccgggtga ccgttttacg tttcacattt cacgtttcac
      181 gcccgcttac gatttcatcc ggggaaagac gctgccgaaa tgcatggaga aggtgccacg
      241 ggccacatcg gcgacgtagc gttcgagggc ttcggtgatc acgggaaagg ccagatcatc
      301 ccagggaata tcttccagcg agaacagttg cacatccagg ctctccgtac ccggtttgaa
      361 ctccggggta cgcatggagc cgcgaaagac catgtggacc tggctgattc ggggcaaact
      421 caagacggca tacagggacg taatctcgac gtcggcatgc gcctcttcaa acgtttcgcg
      481 gatggccgcc tgctcggtgc tctccccgat ctccatgaag ccggcgggaa acgtccaatg
      541 gccggtgcgc ggttcgatcg cccggcgaca cagcagaatt ttgccttccc actcgggaat
      601 gcagcccgcg acgattttcg gattgatgta atggacgacc tgacaggtat cgcacacaaa
      661 ccgcggcagg ttatcgccgg gaggtatttt tttcgacagc ccggccccgc actcgctgca
      721 gaacttcatt gcggcccccg atcacgacaa cagaaagaca gaaatggata gcacaaacca
      781 cgccttcttt gcaccctgcc cccgcggact cgaagcggtc ctcgccgacg aactcaccga
      841 cctcggcgcc gccgacgtga agcccacggc cggtggagtc gcctttcacg gcccccttgc
      901 cctctgctat cgcgtgaatc tgcagagccg catcgccagc cgcattctct tacgtattgc
      961 cgagggctcc tatcgcgacg aacacgatgt gtatgacgcg gcgaacgcca tccgctggca
     1021 ggattggttc acgccgcaac agcgcatcaa ggtgaaggtg agcgcgcacc actgcccgct
     1081 gaagagcctg gattttgtca ccctgcgggt gaaggatgcc gtctgcgatc gctttcaatt
     1141 tgcgaggggc aaacgcccga cggtggatac ccatgcgccg gacatcttga tcgcggtgta
     1201 tttggatgcg tcgacctgca ccttctatgt ggatacctcg ggcgagccac tgttcaaacg
     1261 gggctggagg aggtcggcgg gcgaagcacc gatcagggaa aatctcgccg ccggcatcct
     1321 gcggctttcg aaatggactg cggacacggt gctctacgat cccatgtgcg gaagcggcac
     1381 attcgtcatc gaggccgcct tgatggcccg gcgcgtcgcg ccgggaatcg gccggcattt
     1441 cgccttcgaa aaactgctgt cattcgatgc ccaacgattg aacgacctgc gcacagaact
     1501 caaggctatg gaggtaccca tcaagccggg cctgatccac gcggccgatg acagcgcgca
     1561 cgcgatgaca tcgatcaagg ccaacctcac catcgccggg agcggcgatg cggtcaccct
     1621 tcaacagggc gatgtcctgc aactccccgc tccggccgaa tccggcctgc ttgtcacgaa
     1681 cccgccctac gggcaccgca tgggagaggc ggaaagcctg cgcacgtttt atccgcaatt
     1741 cggcgatcac ctgaagaaac atttttgcgg ctggaccgtg cagattttca ccgccgactt
     1801 aaaactcccc gggcagttgc ggctggcccc ttcccgccgg gtccctctct ttaacggggc
     1861 gatcgaatgc cgcctcttcg agtttcgcat ggtcgccggc agcctgcgca agaagagagc
     1921 caatggcgaa tagcctctaa cccggattat tcatgaatgc cgtcgcgagg cgttcgagac
     1981 ggaagcccgc cgaggctgct cgcacgcgag gccgacgcag gcgtactggc agttcgtcga
     2041 ggaggccgag cgagaacagc ctcgccgggc gagagaatcg gacgccggag caggcattca
     2101 tgaatagtcc gggctcagag caaatggctt atggcttatg gcttatggca agagggcaag
     2161 tagtgaagcg tccctggtcc gccgccacat ctctcattcc tgctcccagc tataggctct
     2221 tgttcccggc cattcgctct tctcttcccg ccatacgctc tacgccatca gctcttgttg
     2281 gctcttgaaa cctcgccgct tgacagcgtc gcgaaagact cctattttca gcatatgaac
     2341 gcgtctcacg cgcccctcga agagctgcag ccggagcagg atctctccct ttcgcgtctg
     2401 atcgtcgtct ccaatcgcga gccatacgaa caccggctgg tcaaaaatca tctgatctgg
     2461 gaacgcacgt ccggcggtct catctcggca ctcgatccgg tcatgcggcg gctcggcggc
     2521 acctggattg cctggggcag cggcaaggcg gatcgcgagg tagtggacca agacatgatc
     2581 gtggaagtgc cccccgacgc ccccacctat catttgcgcc gcgtgtggct tgaaccaact
     2641 gagatcaagg gcggttatca gggctatgcc aatcaggtgc tctggcccct gtgccacctc
     2701 acgctggatc gcgtggccta tcgcaaagcc ttctggcatg cgtaccaggc catgaatgcg
     2761 cgcttcgcgg cagctgtctt cggtgaatta cgcgggaagt ccggttttgt gtgggtgcat
     2821 gactttcacc tggccctggt acccggcctc atcaaggccg cgttgccggc ccagccggtc
     2881 gcagtcttct ggcatacgcc ctggcccggc cctgacgcgt ttcgcattct cccggaacgt
     2941 cgcgaactgc tcgaatccct gctggcgagc gacttgctga tgttccagac ccacaacttt
     3001 ctccattgct ttgccgaatg cgtgaaggaa ttcctgggcg tcgaggtccg gagcgatgac
     3061 catcacatcg aatataacgg ccatacgacg cgactcgtcg cccgcccgat cagtgtgagt
     3121 ttccaagcct ggtcggaacg cgcccgaacg tcgcaggtca cgcgcgccat ggacgtgttg
     3181 cgtaacctac atgtttttca gcccgaggtc cgcatcggtc tcggcgtcga ccggctcgac
     3241 tacaccaaag gtctcctcaa acgattctgg gccatcgatg ccttcttcga gcagtaccct
     3301 cagtatcgcg gccggttcac ctttgtgcag gtggccgtgc cgaccagggg cgatgtcgaa
     3361 gcctatcgca gttatcggga actcatccgc gaaaccgtga atgacatcaa tatgcggtat
     3421 ggcagcatct ccaacgcagg cagtccccag gcctctcgct ggcgaccgat cgagttccgg
     3481 gaaggccgca tcggcatgga caccctcgcg gcctactacc ggatggcaga cctggcgctg
     3541 gtgagttccg tatacgacgg catgaacctg gtcgccaaag aatatgtcgc cagccaggtc
     3601 gacgagaagg gcgtgctgct ggtgagccac atggccggcg cagccgagga actgaccgat
     3661 gccctggtca tcaatcccta cgaccttgag ggtgtcgccg atgccattcg ccaggccatc
     3721 gaaatgccct tggaggaacg ccgggagcgt atgcatcgca tgcggaccta cctcgaaacg
     3781 catgacatcc gggcctggtc ggacgagtgt ctgcgcgatg caggaatcct cccacccgat
     3841 gatagcccca cgcttgaata gccgacgccc acatcaacgc gcctgtggag caggccagtg
     3901 atcgatactg cctcgtcgcc acacgacatc acatcatcgc ccgtccgccc acggcgtctg
     3961 gaccggcatc gtacagacac ccaccgcttc catatcctgg gccgattact ttccctggtt
     4021 gtcaccatga gcctctgtct tgctttcggc ggctgtgctc aaatcgcgca tctgtttgcc
     4081 gtcactcaac cctcagacca accggtggat cgacaacaac aggcaatcgt gacgcgcctg
     4141 aaagccctgg tcgagccgga cctccaggac aatctcaata cggcgctccc ggcggttcgg
     4201 aagagcctgg ctgatcagac caacaagatt gcgaagttca aacgcaacct ggtcctacgg
     4261 acgctggccg atccctggga cggcctggca gccgccgaac aggccggtct attagcggcg
     4321 actacggccg aatcgggcgt cgaaggactg ccggccgtga tcgacatcct ggaaggcggc
     4381 atggaccgta ccggcgcctc ctttgcgccg ctgacctttc cggtcacact cgccactgag
     4441 gatctcctga cctttctcac cgacgtactc gaacaggcct atcaggaccg cgaacatgcg
     4501 ctccgccatc tctcgccaca agaccgggat ttcttgttca cgcaggctcg cccactggtg
     4561 gaacatttca ctcctcaggt cgccccgccc gctcaacttt cagaggccga agccgcagcc
     4621 agatacgcga ccctgctcat gcagcaggtg gactacgcct cgctcatcag cgccgcgcaa
     4681 cggctggcgc ggctcggcaa ccggaaattc ctacagcaac tcgatatcgc gttgcacaac
     4741 cgcaaaccga tcggccagac cgtgcccggc atcaccggag atctgctcct ggctcaacag
     4801 acgtcgtacg gactagtagt agtgggaggc cacggcccga acacctatga cctcgaaaag
     4861 ggcgccgcgc tgatcatcga cctgggcggc aacgacacgt atcgtggaac gatcggggca
     4921 agcccgaaca gcgatctggg caacagtgtc gtcatcgact tgagcggcaa cgatacctat
     4981 cagccggccc cgctgggcct tgccaccgga cgcgcaggaa tcggcatcgt gatcgatcat
     5041 agcggcgacg atacctatcg gctggcaccc ggctccggcg gggtcggcct cgcgggcctc
     5101 ggcatcctgt atgatggaga aggacaagac atctacgaag gaaaccggtt cacccagggg
     5161 gcggccttcg gcgggttcgg cttactggtg gatcgcgccg gagacgaccg gtataccagc
     5221 ttcggctacg cgctgggctt cggagcgccg ttgggtgtcg gcgcgttgat cgacgtatcg
     5281 gggaacgatt tctacgaatg cggcgggcgt taccccagtg cgtataacga aaccgacgcc
     5341 ccgaatgcgc atccgcagga ccctgtgttt caatacgatt gtttcgggct tggaaccgga
     5401 tccgggcttc gggtcttcag caaacaaccg gcccaccgcg cgctcagcct ggccgggggg
     5461 tggggactct tcatcgatct cgatggtcgc gaccgttatc gcagtgcgaa cttttcccaa
     5521 gggcacgggt attttttcgg tctcggcgtg aagctggatc tgaacggtga cgacgaacac
     5581 caggccgcac gttatggtca tggcaccgcc gcacacttcg gcgtgggcct gaacatcgat
     5641 taccagggca aggaccgcta tggctccaaa ggtcccttct ataacggtgg cgcagcctgg
     5701 gacggcagtg tcgccctcgc cgtagacggc ggtccggaca gcgattttta cgatctcccc
     5761 gcctcgactg gcttgggaat gggtgacctc gggggatggg ggctgttcat tgaagaaggc
     5821 ggtgcggatc aatatgcggt gagccggggg ctcgggcaag gggctgagca cagcctcggc
     5881 gcatttttcg acctggaggg aagagacgat tattcctccg tttccccgcc cgccaaaggg
     5941 gtgcgtcccg aacggcttaa tgggaaaacg tttattgaaa acgcgggcag cctcttcata
     6001 gaccgatagc aggatggtga aaatgtccgc cagctttgtt ctcgcatcgc gcagacgctc
     6061 aacgtaccgc gagggtacgc ttcgactctt ctctcgctgc ggcctcgctg gacggccttt
     6121 ttgaccatcc tgcatgctcg ctccgcgttg tctccaactc ccgcgctctc ccctttcctg
     6181 ccctgcgctt gactccccct ctcttatggc tatcttaacc ggtaagaacg agcgtgaccg
     6241 tgccttctgt acccgtcatg ccgctctcac ccgatgacca tccgagcaca gcggaggata
     6301 aaatgaacga atcgaatgcc ccgatactca cgcatttgcc cgtgttgccc ttaaagcgga
     6361 cggtgttgtt ccccggcacg atgatgccct tgaccgtcgg ccgggaccgt tccatagccg
     6421 cggtcgaggc ggcgctgaag accgaggaca aaacgctcct cgtcgtggcc cagcgggatg
     6481 cgcaaaccga tcagcccacg ctggaagatc tctatcccat cggcaccaag gccgtgatca
     6541 aacagacggc tcgaacgcct gaagggcact acaacatttt gatccagggg ctggagcggt
     6601 tcgtgctgct gaagctcgac cagatggatc cgtatctcca ggcccgtgtg aaacagctcg
     6661 ctcctccgag tgagcagagc acagaagtcg aagcactgca tcgggccatt ctggacatca
     6721 tcaccgaact gccgaaactg atccagactc ccggcgtgca tgaagcagtc gccgccctgg
     6781 gcacggaaga agatcccgtg gtcctggcct atcgcatcgc gtccttgttg aatctcacct
     6841 tggacggcga gcagcagttg ttggcggccc ctacccgggc cgatctgttg cgcggactct
     6901 atgcggcgct gtcgcgggaa gtccagattc tccagttacg cgacaagatc accagcgaag
     6961 ccagagagaa actgggcaag acacagcggg aatatctcct ccgcgagcag ctcaagacga
     7021 ttcagcagga gctgggcgag gggaacgggg aagaggatga agtcggcgtg ctgagatcca
     7081 agattcacga agccgatctc cctgaacatg tccggaaaga gacggaccgt gaactggcgc
     7141 gcctcgccaa gacgccgagc gcgtcgcccg agcatcaggt gatccggagc tatttggagc
     7201 tggtgctcga actgccgtgg aacaagtcct cggaagaagg actcgatctc gctcatgtcc
     7261 gtcaggtgtt gaacgaggat cactacggta tcaaggaagt gaaagaacgc attgtggagc
     7321 acctggcggt cttgaaactg aacccgtccg ccaaggcgcc gatcctctgc ctcgtcggcc
     7381 ctcccggcgt gggcaagacc agcctggggc agtccatcgc caagtccatg gggcgcacgt
     7441 tcgagcgctt cagcctcggc gggttgcatg acgaaggcga attgcgcggc caccgccgca
     7501 cctatgtcgg ggcccttccc ggccggatca tccaagcggt gcgacgagcc ggcgtgaaca
     7561 atccggtcgt catgctggat gaagtggata aactcggacg cgatttccgc ggcgatcccg
     7621 cggcggcgtt attggagatt ctggacccgg cgcagaacca tacgttccgg gaccactatc
     7681 tggatttgcc gttcgatctg tcgaaagtgt ttttcatcac aacggcgaat acgctggaga
     7741 cgctctcgca acccctgctc gaccgtatgg aaatcatccg gttgaatgga tacagcgagc
     7801 gggagaaacg cgaaatcgcc ctgcggtacc tctggccgag acggttgaag gaagccggtc
     7861 tgcgagccga ggacgtgaat cttcccgaag cggtgctgga ccatatcatc ggccgctata
     7921 ccagggaatc gggcgtacgg cagctggaac agatgttggg ccgcattacg agaaaggtcg
     7981 ccgtgacctt cgcggatcgg ccggccgatg ctccggcgag ccctgtggac atcacgcaac
     8041 cgctcctgga cgagtggctc ggacaggaac gattccagcc ggaagaggcg cgaaagaatc
     8101 tgccggccgg agtcgccacc ggtctggcct ggactccgac aggcggtgac gtgctctata
     8161 tcgagacgac cctgctgccc ggcagtcacg agttgaccct gacgggacag ttgggcgatg
     8221 tcatgcagga atcggcacgc gcggcgcgca gctatctctg gtcgcatgcc gagagcatgg
     8281 ggctggatat ctcgcgcttc aaacgcaacg gggtgcacat ccatgttccg tcgggcgcca
     8341 ttcccaaaga cggcccctcg gccgggatca ccatggcgac cgcgctggct tcggcctatg
     8401 tcggcaagcc ggtgcgcagc gatacggcga tgacgggaga gatcagcctg accgggttgg
     8461 tgctgccggt cggcggcatc aaggagaagg tcctggccgc ccatcgcgcc gggatccggc
     8521 ggatcattct gccgaaggcc aacgaaagag acttgaaaga agtgccgcag gaagtgcgcg
     8581 atgaactcac ggtcattcca gtggaacgga tcgaagaggt tctcccggcg gcgttcaatc
     8641 aggatcctgc gtcctcacct gaacggggtg aaccgttggc gagcgcgacc gcctcgtaag
     8701 atccagggcc ccggctgaac agccggggcc cctcgccttt cctccctgcc ttcctgcttc
     8761 aactgccgct tgctttgcag actcctgcac atccgattac cttgcccttc cgtaatcact
     8821 tatgtcagga tcaaattgtt gttcgacccg tgctcacgtc ctaccacgac gtgaaggaga
     8881 gtgaccatgt cgcccaaaat tgaaatcggg aaaatcgtga aggtgaccaa gaccgacgac
     8941 gaatggaaaa cactgttgtc gcccgcggcc tatcaggtcc tgcggcatga agataccgag
     9001 cgggccttta cgagtccgct ccacgagaat catcaggcgg gcatctatca ttgtgcgggc
     9061 tgtgacctgc ccgcctattc ctcggagcat aagttcgata gcggcaccgg ctggccaagc
     9121 ttctggcaac cgattgaccc caaggtggtg gagaaacgca cggattccag gtttttcatg
     9181 acccgggtgg aagtccactg tgctcaatgc ggcggccatc aaggacatgt cttcgatgac
     9241 ggtcccagac ctaccggcct ccgctactgc atcaacggcg tttcactgaa gttcgttccg
     9301 gcctgattgc gatggcctat gtcggcgggg aacaggacgc aggccgatca ttacgacggc
     9361 cacagcgtta ccactgtagc ctggcgacgc accttgcaat ttgtcgctga accctcctag
     9421 actgcgcatg ccatggcgta cttctccttg agacgaccgc tcctgggcct cttcctggcc
     9481 gggactgcag tgctcggttg ttcacaatcc tccgacgtga tctcctccaa tctcaacgcc
     9541 tgcttgatcg tcacagaggc cgaggtcgaa ttggccatcg gaacccccgt cacgccgggc
     9601 ctgcgccaga atgatcgcca gtgcctctac caggcaaaac gcaatcctca ggaaaccgtc
     9661 accgtagagt taaaccccgg tcctgacgga gacacaaaag gtctgtttca cgatcagcgt
     9721 tcaaaggccg gtcaccagcc ggttcccggg gtcggcgacg gtgccttcac cctgcggtcg
     9781 cctatcgggg ggattcagtt gacgtttctg aaggccgatg ccctcgtcac gctgtcgctc
     9841 tcgtcctcga aacaacccaa cgcgcaggcg gccgtgacca acctcgccaa ggtcgccgcg
     9901 accagactcg gcggacccct tcgaaccggt ccggcggcgt caaccgagcc agggttgacc
     9961 gccactcctt cccaatgggc cggtgattgg tatggttgcc cgcccgtcgg attgatgtat
    10021 gggaaaggcc atcttgcgct gagcgagggc ggcacctggt ccttgacgac tgccgtattg
    10081 atgccgggca ccgtgaccgc agaccgggga cgctggcagg cggaatccta tcaggagatc
    10141 ctgcacggaa cctatcaagt ggccgggaac gaccgcttct caaccaccgg cattctaagc
    10201 gtcacctggg acaaacttgc caagaatcag gggccgaaca aattcgaccc gctgctctgg
    10261 caatcatttg cgtccgtccc gcgcaaggtc gccgtcaaac ggttgccacc ggtcgagccc
    10321 tccctcgtgg gcacgtggga aggttccgcc aagttcgtcg atcgacagga agaattcgtc
    10381 tggaccatca cgccggcaaa cgtctcggag ttctacaggg caacctcgca gtcgggtcgc
    10441 atcgagaagg agcaggatcg gtttcgcttg atcgtccccc aaggcaaaac tcctcccctg
    10501 tttgtaaggg tgttggcgca ggacaagctg gagctgaccg acaacagcgg cacggtttcg
    10561 caatggaacc ggaatgagaa actgctctcg cgctgttgat ctcccgattg acctcaccag
    10621 cgacgccagc ttacgcccct cggcttgacc cgtttttttc tgttcggtag actgttggca
    10681 tgacacgttg gctcatggtg acgctgctct gtactgcgcc gctgctcccg gccgtgcaga
    10741 gccaggcagc gcccggcgac acagcacaga ctcccgactc gatcgaatca ttcgatctgc
    10801 caactccaga cggtctccgc agcctttaca accagaccct cgccgcctta caggaataca
    10861 tcgagattga tggaagccta ccgcaagatg gtgagacaca ggcacgagtg ggagagtttc
    10921 gactcaaagt ctttcctcag gggaaatccc gttcgcagga acacctaagc gccgagggat
    10981 cgttccgcct ctcgcccgac cccggacaac aggagttctc ccttcgtttc aaatcctcca
    11041 aacgcccgtc gacgcctcag ccaagcggcg acatcattta gccgcatcga acagtaacaa
    11101 ctccgctgta tggcaaaatc ggtggaccta aggccgtcgt catcgtccgt cgttcgtgac
    11161 acgtcgtccg tctctcgatg cagaacagga gcaaaatgca tattgcttat gtcctatggt
    11221 tgagcaagac agcaacccgc ggcgttctgc cttcatccag aagccatacg ctcttctttc
    11281 tcgccatcgc tatcagctcg gtgcctgatg agatacgctc cacgatgcac gcttcacgga
    11341 tgtcgagaat gccggcggca gagcaggtca acagcctgct agaaggtgaa gccgatgccg
    11401 ccggagataa tatgagcccg atagttcatg gtgaatgacg gaccacgcgc accgttcgcg
    11461 acttcactct cccactgata attcgccaca aaatatttgt attccgtgaa gaggaacatg
    11521 cgctcagtga cgttggcgcg caccccgcct agaaactgat aggcgaaggc gccgtcggcg
    11581 gcattctcat gcaccgtgcc gatggtgctg tgctggagat tcaaatcccg cgcaaaccct
    11641 ccggacaacc ctgcaccgat gccgatatac ggttgaatcg tctcgccggg gtagcgcagc
    11701 aatacattcg ccatgacgtt gatggactgg agacggaaat tcgcgctcct cgtcacaccg
    11761 cctgtcgtgg ccgaaggcgc attgaccttg ccatcgagtc cggacaggtc cgcctccaat
    11821 ccgacgaacc ggccggcgaa cgccggaaaa atcccaactt tcaacccgcc tcccaacccg
    11881 ccctgcacgt cggtattcgt gaagggatcc tcctgcagcc tcaccggacg attcagcggc
    11941 cggcttccct ggagatagac actgacatag aactcggttt tctccccggc ctgtgtggcc
    12001 tcgggagatg ttgccggagc cttggctgcg ctggaagccg gttccgctgc cgacgcagcg
    12061 gcatgatgtg tctcgaacaa cgaacagctt aatatgaacg cggcgaggaa ccattgcgct
    12121 gatttcatgg gtcactcctg tgggctaaac ggcctagcag aactactcag ctctgcaata
    12181 ggccactcat accacgggga ctcttgacga caaactgatt cggtagtttc tgcagcgagg
    12241 tgagaacgcg taagccaacc ggtcacgtgg cgaggccgtc gatgatcggc gaaaggcaga
    12301 tcggagcagg ccctcttcag gagagaaatc ccttgatgac atccgccggc atcaggccga
    12361 cgagcaacgt tccgtccggg cgttgcagga gcggcacgcc ttgatgatcc gcctcagccc
    12421 actgatcatt ccaggcccaa agaatcgcat ctacggtggt ggcctctgtg ccgacaatct
    12481 gcgcggaggc aaacccctga cgttcggcct cccgctgtag cagcgtctca tcggagagat
    12541 cctgcccgtc cacccagaac gaccgataca acgatcgcac aaactccccg ccccgaagcg
    12601 ggtccatgtg aagcgcgcgc gcagccgcgg caatcgcccg cccggtattg ggttttcccg
    12661 gcggcacagc gatcggcaac tcgggcgcga gccggcggac catctgcacc tcctgcttca
    12721 gttcagctgc caggtgccct gcccacccag ccatcgggac cggcagatgc ggtgcatgct
    12781 gaaccccttg ccaggagatg cgatccatca cgcccaatgc atgaaggcgt tcgtgcatcg
    12841 cgtaacaaaa tggacagttg aaatcgctgt agagccgaca cgtattcatt tcaattccac
    12901 aagatgaatg aaaaccaagg aagtcaggat ggtcaaaaag gtcgtccagc gaggccgcag
    12961 caatgtgagg aggcgaggcg tacccttgcg gtacgttgag cctcggaggt tcacgacgcg
    13021 ctgaataaag cgcgtcacgt ttgtgaacgc cgccgagacg gtgaggcggc agtgtcttgc
    13081 gagaacgaag ccggcggcct tttcaccatc ctgtcagccc ggattattca tgaatgccgt
    13141 cgcgaggcgt tcgagatgga agcccgccga ggcttctcgc acgtaaggcc gacgcaggcg
    13201 tacttgcagt cc
//
LOCUS       DAORRQ010000083        12990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_235_length_12990_cov_123.728952, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000083 DAORRQ010000000
VERSION     DAORRQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_235_length_12990_cov_123.728952"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK224_18110"
     CDS             <1..396
                     /locus_tag="PK224_18110"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17716.1"
                     /translation="AQSPGDINRYKVEPYVVAADVYAVPPHTGRGGWTWYTGSAGWMY
                     RLIVESLLGVRLEHNRLRFVPCLPLDWKTFKVHYRFRETMYHITVRQSGTEHQAMSVT
                     VDGIQQADHAIRLVDDRREHSAEVTIPSA"
     gene            683..1462
                     /locus_tag="PK224_18115"
     CDS             683..1462
                     /locus_tag="PK224_18115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRB17717.1"
                     /translation="MLIDTHTHLDDARYESDREAMIARARAAGVESMITIGCDLATSR
                     SAVTLANQYPFVYASIGVHPHEVKHIEDGWYDEFRRLARDKKVVAYGEIGLDYHYNHS
                     DPELQRTRFREQIQLARELKLPVIIHTREAQDDTIRILKEERASEIGGVFHCFSGDAW
                     LAKDAIELGFYLSFSGILTFQNATMLREIAKTVPVDRLLIETDCPYLTPVPHRGKRNE
                     PAFVRHVAELLATITADNVPSTAEDVARRTSENARRLFKIP"
     gene            complement(1449..1982)
                     /locus_tag="PK224_18120"
     CDS             complement(1449..1982)
                     /locus_tag="PK224_18120"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRB17718.1"
                     /translation="MATHLLVDGYNLVGSAGMALPAGSGRLEAARETLLKNLAGYRQR
                     KGHAITVVFDGWQGGLGAEHREFQSGVEVVYSKRGERADQVIQRLARLYGRDCAVVSS
                     DHEVVNSARAAGAFVIGAAEFRAKLQDRSSSLSTVAFKELDRGDLDRVKRSKDKGGNP
                     RKLPKSQRKRNHQLREF"
     gene            2076..3404
                     /locus_tag="PK224_18125"
     CDS             2076..3404
                     /locus_tag="PK224_18125"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRB17719.1"
                     /translation="MSHVASLIRHLLTFAFACVLFGTGISSGGEIQTEPSWPTVPQVA
                     AEEPSPRFLLVKQERAGASRYPIIVRDLRTWSTADGTRLVLDLNHKTSFSETHLRNPD
                     RVLIEVNNAILGKSSRQRVSGGTIPQSFQIAQSRPRVVSITLTRTQNSRYKVFSLDNP
                     DRLVIDITQRPKDQIRQIGETSATSPQAPAVPLPTPPAIPSPTVTEPARPVAPKPASP
                     VQTIVIDPGHGGKDPGTIGQHGTTEKEVTLKVGLLLKSLLSTVPNTRVLMTRERDAFI
                     ELEDRAKFANGKDADLFISIHVNSHPHKGVRGLEIYHFGEAKDQRALEVAARENGTPL
                     NNTGVGWEYIVADLLTSKKIEHSQELAWTAKQAMVTNLNGRYSTIDHGVKTAPFYVLR
                     FTTMPSILAEIAFMSNPAEEEQMRSQPFLAHIAESIFEGVKTYLHTNPPR"
     gene            3452..4213
                     /gene="truA"
                     /locus_tag="PK224_18130"
     CDS             3452..4213
                     /gene="truA"
                     /locus_tag="PK224_18130"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRB17720.1"
                     /translation="MTTFKLVLEYDGTQYAGWQRQLNVPTIQATVEDALAAIAQTRLT
                     LVGAGRTDAGVHAFGQVASFRTDRRLSQREWLRALNAHLPADISAISVEAVPDHFHAR
                     YSAKGKLYEYHLMNRPERAPLLRERAWMLYKPLDFAAMTEAAACLTGSHDFSSFETAP
                     TDNENPQCRLQQADLRRQGDLIILSFYADRFLKQMVRAMVGTLVEVGQGKRTAPDMRT
                     VLAARARAAAGRTAPAHGLYLVRVDYTEGSIPSLP"
     gene            4295..4612
                     /locus_tag="PK224_18135"
     CDS             4295..4612
                     /locus_tag="PK224_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HRB17721.1"
                     /translation="MKLLCTTLSVLMLAAVLATPQLSYAGAQQNKMKTCNADANAKGL
                     TGEGKGDARKAFMKECLSAKPEKAAAGASQQNKMTTCNKDAKAKKLAGDERKKFMKEC
                     LSN"
     gene            complement(4758..5408)
                     /gene="cysE"
                     /locus_tag="PK224_18140"
     CDS             complement(4758..5408)
                     /gene="cysE"
                     /locus_tag="PK224_18140"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRB17722.1"
                     /translation="MFKTISQDLQAIFDRDPAATSRLEVIATYAGFHALLAYRLAHWL
                     KGHRVPFLPRAISQLARWLTGIEIHPSAKIGERFFIDHGMGVVIGETAEVGDNVTLFQ
                     GVTLGGTGKERGKRHPTLGSHVVVGAGAKILGGITIGDNVKIGANSVVLKSVPPNSTV
                     IGVPARIIKSQGERLPDATMDHTNMPDPTADRFAALELELIELRKKLENRDPNSPR"
     gene            complement(5448..5930)
                     /gene="ispF"
                     /locus_tag="PK224_18145"
     CDS             complement(5448..5930)
                     /gene="ispF"
                     /locus_tag="PK224_18145"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRB17723.1"
                     /translation="MTAARVGCGWDIHPMVAGRKLILGGLEIPHHKGLQGHSDSDALV
                     HAICDALLGAMGEGDLGRHYPSSDQRFKNISSLKLLEDVVQKLRAKGYRLANVDSTII
                     AQAPRLSPHLAAMQKIIAGVLEVDPDLVNVKVKSGEGLDAVGREEAIAAQAVCLIERG
                     "
     gene            complement(5927..6670)
                     /gene="ispD"
                     /locus_tag="PK224_18150"
     CDS             complement(5927..6670)
                     /gene="ispD"
                     /locus_tag="PK224_18150"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRB17724.1"
                     /translation="MSARNAKPSLAGPRTVALVPAAGRGLRMGGHIPKQFLALGGRPI
                     LAQSLLVLQASPLIHEIILAVPESERQYCLDHIVATGEFSKVTKVVPGGAQRQDSVRH
                     ALAEVSQETEMVLVHDAVRPFLTEDMIRRVVAAAVEHGAAIIALPMRDTVKYVGAGGV
                     IERTVDRRPLWLAQTPQAFRRDWLEEGHHKAFLAGVQATDDAHLVELIGKPVVVVEGS
                     GENIKVTRPEDLVIGEAILSSRTAARSAE"
     gene            complement(6790..7815)
                     /locus_tag="PK224_18155"
     CDS             complement(6790..7815)
                     /locus_tag="PK224_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRB17725.1"
                     /translation="MVTRAIFVLLSALAGMALFLRAQDPSREFLLMGLGMGAVAGGLI
                     LAGEYALRRLSFGIIVGGAVGLAGGLILTGLVEWVGSAVFDVETFLFHIGGLVFLLGL
                     PYLGLAMGARFGNEQFPGLDQGPTAAGASHASLKVLDTSVIIDGRVADLCETGFLEGP
                     FLVPHFILNELQHIADSSDSLKRARGRRGLDILNKIQKMPGIDVRIIDEDFPHVKEVD
                     AKLVVLAKKVGGRIVTNDLNLNKVAELQGVRVLNINELCNALRPVVLPGETIRVFVLK
                     EGKEAGQGVAYLDDGTMIVVDNARRCIGRNVDVTVTSVLQTTAGRMIFTRLKEESERE
                     EYQVARG"
     gene            complement(7866..9410)
                     /locus_tag="PK224_18160"
     CDS             complement(7866..9410)
                     /locus_tag="PK224_18160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRB17726.1"
                     /translation="MNDFQIPTEQQPPRRSWIFPALLLIAGVLIGVILTSDLGWLPTG
                     HAVPEPVPPPVATPVSTAVQPTLPGAGGQSFVDVAKVVKPAVVNIFATRNGSSEEGKG
                     TPFEDPFFRRFFGEEWMKRFEAPKERKERGLGSGVIVDPNGLIITNNHVVSKADEIKV
                     FLSDKREFKAKLVGTDAKTDVAVLKIEAGGLPTVAWADSDKLEVGEFVLAVGNPFGLT
                     QTVTLGIVSALGRAAGIAEYEDFIQTDAAINPGNSGGALVNVRGELVGINTAIYSQSG
                     GNMGIGFAVPSNMAHSIMEQLVQHGKVVRGWLGVSIQELTPELSSQFGVPKDVKGVLV
                     SDIMDDSPAKKAGFERGDVIVEYDGKPMDSPAHLRNAVAQTVVGKKVTVKLIREKKPK
                     SIELTIAEQPKNLAQAGVEEGGESVAPAGLLSDVEVRELNTELAGRYGLKSSDHGVVV
                     VRVKPGSPAEEAGVREGDLVLEVNRKAVGTLKAYEHVAANLPKDQAVLLLLRRQGRAI
                     YLTLRP"
     gene            complement(9438..10361)
                     /locus_tag="PK224_18165"
     CDS             complement(9438..10361)
                     /locus_tag="PK224_18165"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRB17727.1"
                     /translation="MSNRVQEILSWYESDNAGTKTNIARLLNHGKLAGTGKLVILPVD
                     QGFEHGPARSFAPNAGGYSPHYHFQLGLDAGCNAYAAPLGFIEAGASHFAGQIPLILK
                     LNSHDTLHDEKDPMPSVTGSVRDALRLGCSAVGFTIYPGSCHCNAMYEQLRAIAEEAK
                     SCGLAVVVWSYPRGSGLSKEGETALDVVAYAAQIAAQLGAHIIKVKLPTAHLEQAAAK
                     KVYEAEKVPISTLAERVKHIVQSSFDGRRIVIFSGGAKSDEKTVFEEVRAIRDGGGFG
                     SIIGRNSFQRPKAEAVKFLHTIMGLYAGEKP"
     gene            complement(10398..11390)
                     /gene="fbp"
                     /locus_tag="PK224_18170"
     CDS             complement(10398..11390)
                     /gene="fbp"
                     /locus_tag="PK224_18170"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRB17728.1"
                     /translation="MAKFPITLSRFIIEQQAAHPEATGEFSVLLTQIGLVGKMIAHDL
                     RRAGLNKILGTTGETNIQGEVVKKLDQIANDTFVRVFEHSGLVCVLASEEMEKPLKMV
                     HEGAKYMLLVDPLDGSSNTDVNMPLGAIFSIRRSKTGQSVEDELLQKGTEQIAAGYVL
                     YGASTMLVFTVGQGVHGFTLEPSIGEYLLSNENIRIPSKGKVYSVNEGNYHRWPSGTQ
                     KYLDSLKVQDKATGRPYSGRYSGCLVADVHRILLGGGIYLYPGENDKPEGKLRLMYEG
                     NPLAMVVEQAGGKATTGMMRILDVEPKALHQRVPLIIGSAEDVSQAEAYIQGRA"
     gene            complement(11524..12336)
                     /locus_tag="PK224_18175"
     CDS             complement(11524..12336)
                     /locus_tag="PK224_18175"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRB17729.1"
                     /translation="MSRYSASLRLSLFLAVTILATVVASAPLLSYATPDGQILTPAAL
                     PFANGERLSYDVTWLGMRAGIATMIVQEGPVEGPRPQLTFAMTARSSPTVTKFYPVDN
                     RGVSVVDRESFLPRHMTFARREGKRFNDFDYTFRHGEGLVTAVKDGKTDELSIPPDAQ
                     DAMSCLYYVRKVLPFVPGASLAMTVHHDKKNYKMEVRVEALETVEGVWGKRQTARVVV
                     IMPFQGIFLNEGNIRVWFTADEHRVPLRMKAKVVVGSIVAELTEGYEATSQP"
     gene            complement(12338..>12990)
                     /locus_tag="PK224_18180"
     CDS             complement(12338..>12990)
                     /locus_tag="PK224_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HRB17730.1"
                     /translation="WGDRLMIVYARDILTQRPGTTFISEVKASQCLYDDIAAKGGRPI
                     MWKTGHSLMKAKLKAESAVLAGEMSGHMFFADRYFGFDDAIYASCRLVEILAKTKRSL
                     SSLVADLPQTTVTPEIRVDCPDTLKFQLVDQVRTQLAAYLAEGQPVGASNLRMRELVT
                     IDGVRAIFEDGWGLIRASNTQPALVLRFEAPSPARLDVIRATIETELAQARRVLST"
BASE COUNT         2742 a         4195 c         3505 g         2548 t
ORIGIN      
        1 gcccagtcac ccggggatat caaccggtat aaagtcgaac cctacgtggt agcggcagat
       61 gtctatgcgg tcccacccca cacaggccgt ggcgggtgga cctggtacac cggctcggcc
      121 ggatggatgt accggctgat cgtcgagtca ctcttggggg tgagactgga gcacaaccgg
      181 ttacggttcg ttccttgcct gcctctggat tggaagacct tcaaggtcca ctaccgattc
      241 agggagacca tgtatcacat cacggtgcgg caatcaggca cggagcacca ggcgatgtcg
      301 gtcacggtcg atggtattca gcaggccgac catgcgattc gacttgtgga tgatcgtcga
      361 gaacattcag ctgaggtgac catcccatcc gcgtgagaga actgagcgcg ttggcatacc
      421 gggacgctct ggtggctggt ggctcccgag gtttcccttt cgtccgtcgt tcgtgaagcg
      481 tatctcggtg ggaataagag cgtatggctg atggcttata gctggtagta aagacgccca
      541 ctctctattt cggatcatac gctatgagct atacgccaga ggctcttttc tgcggacgag
      601 agacgcttca cgggtgtaga gagcgccatc ggcaaacttt ttcagcatcc tacgagcgga
      661 caaccatgtt aggatgcgcc ccatgctgat cgatacccat acccatctcg acgatgcgcg
      721 ctacgagtcc gaccgcgagg ccatgatcgc ccgtgcgcga gcggccggcg tggaatcgat
      781 gatcaccatc ggctgcgacc ttgccaccag ccgatcggcg gtcaccctcg ccaaccagta
      841 ccccttcgtc tacgcgtcga tcggcgtcca cccccacgaa gtcaaacaca tcgaggacgg
      901 ttggtacgat gagtttcggc ggctggcgcg cgacaagaag gtcgtggcct acggcgagat
      961 cggcctcgat taccattaca accactccga tccggaatta cagcggacgc gctttcgtga
     1021 gcaaattcag ctggcacgtg agttaaagct cccggtcatc atccacacca gggaagcgca
     1081 ggacgatacc attcgcattc tgaaggaaga gcgagcctca gaaatcggcg gggtgtttca
     1141 ctgtttttca ggagatgcct ggctggcgaa ggatgcgatc gagctgggat tttatctgtc
     1201 attctccggc atcctgactt ttcagaacgc caccatgttg cgcgagattg ccaagacggt
     1261 cccggtggat cggctgctca ttgaaaccga ttgcccctac ctcacgccgg tcccgcatcg
     1321 cggcaagcgc aacgaaccgg cctttgtgag gcatgtggcc gaactgctgg cgacgatcac
     1381 cgccgacaac gtcccctcga ccgctgaaga cgtggcccgc cgtaccagcg agaacgcccg
     1441 ccgcctgttc aaaattccct aagttgatgg tttcgtttac gttgtgactt cggcagcttg
     1501 cggggattcc cgcccttgtc tttggatcgc ttcacgcgat cgagatcgcc gcgatccagt
     1561 tctttaaagg cgacagtcga caggctggac gatcgatcct gcaacttggc gcgaaactcc
     1621 gccgccccga tgacgaacgc gccggccgcc ctggctgaat tgaccacttc atgatccgag
     1681 gacaccaccg cgcagtccct gccgtacaga cgggcgagac gttgaatgac ctggtcggca
     1741 cgttcgcctc gttttgaata gacgacttcg acgccggatt gaaattcacg atgttccgcg
     1801 cccagcccgc cctgccagcc gtcgaagacc acggtaatcg catggccttt tctctgacgg
     1861 tacccggcca ggttcttcag gagcgtctcg cgcgccgcct caaggcgtcc ggaaccagcc
     1921 gggagggcca ttccggcgct gcccacaagg ttatacccat cgacaagaag atgtgttgcc
     1981 atctacaatt aaatggtttg aggccttttg ttgacattac cataaacatc tgagaaaagg
     2041 cctagagggt atcctatcga gaggagttcc ggttcgtgtc ccatgtcgcc tccttgatcc
     2101 gtcacctcct gacgttcgcg ttcgcctgcg tcctgttcgg cacggggatc tcatcgggtg
     2161 gcgagatcca gaccgaaccc tcctggccga cggttcccca ggttgccgct gaagagccct
     2221 ccccccgttt cctcctggtc aagcaagagc gcgccggggc gtcacggtat ccgatcatcg
     2281 tccgcgacct gcgcacctgg tccaccgcgg atggcacacg cctcgtgctc gacctgaatc
     2341 acaagacgtc cttctccgaa acgcatttgc gcaaccccga ccgggtgctc attgaggtga
     2401 acaacgcaat cctcggaaaa tcctcacggc agcgcgtctc gggcgggacg attccgcaat
     2461 cgttccaaat tgcgcaaagc cgcccgcgcg tcgtcagcat cacgctgacg cgtacccaga
     2521 attcccgcta caaggtgttc tcgctcgata atcctgaccg cctcgtcatc gatattacgc
     2581 agcgtccaaa agatcagatc cggcagatcg gtgagacctc ggcgacatcg ccacaggccc
     2641 cggccgtccc cttgccgact ccgccggcga ttccaagccc cactgtcacg gaaccggctc
     2701 gcccggttgc acccaagccg gcaagcccgg tccagacgat cgtcattgac cccgggcatg
     2761 gaggaaaaga ccccggcaca atcggacagc acgggacgac ggaaaaagag gtgaccttaa
     2821 aggtcggcct gctcttgaaa tccttgctca gtaccgtgcc gaatacacgt gtcctgatga
     2881 cccgcgaacg cgatgccttt atcgagctgg aggaccgggc caaatttgcg aacggcaaag
     2941 acgcggatct gttcatctcg atccacgtca attcacaccc gcacaagggc gtccgtggtc
     3001 tggagatcta ccatttcggc gaagcgaagg atcagcgcgc cctggaagtg gccgcgcgag
     3061 aaaacggcac gccgttgaat aataccggtg tgggctggga atacatcgtc gccgacctcc
     3121 tgacatccaa gaaaatcgaa cactcccaag aactcgcctg gacggccaag caggcgatgg
     3181 tcacaaatct caacggtcgg tacagcacca tcgatcacgg ggtgaagacc gcgccatttt
     3241 acgtgctccg cttcaccacc atgcccagca ttctggccga gatcgccttc atgtcgaatc
     3301 ccgccgaaga agagcagatg cggtcacagc ccttcctggc gcacattgcg gagtcgattt
     3361 tcgaaggggt caagacctac ctccacacca atccccccag atgacaggcg ccttcccgtt
     3421 gagctagagt tccttctttt ccttccagag tatgaccaca tttaaactcg tcctcgagta
     3481 cgatggcaca cagtacgctg ggtggcagcg ccagctgaac gtgccgacca tccaggcaac
     3541 ggtcgaagat gccctcgcag caatcgccca aaccaggctc acgctcgtcg gagcgggccg
     3601 gaccgatgcc ggggttcatg ccttcggaca ggtcgccagc ttcaggaccg atcgcaggct
     3661 ctcccaacgc gagtggcttc gcgcgttgaa cgctcacctc cccgccgata tcagcgccat
     3721 ctcggtcgaa gccgtgccgg atcattttca tgcgcgctac tcagcgaaag ggaagctgta
     3781 cgagtatcat ctgatgaatc ggcctgagcg agcacccctg ctccgggaac gagcctggat
     3841 gctctataaa ccgctcgact ttgcggccat gacggaagcc gccgcctgcc tgaccggctc
     3901 gcacgatttc tcctcattcg aaacggcgcc gaccgataac gagaatccgc agtgccggtt
     3961 acaacaggcg gatctgcgcc gccagggaga tctcatcatt ctctccttct atgccgaccg
     4021 gtttctcaag cagatggtcc gtgccatggt gggaaccctg gtggaagtgg ggcagggcaa
     4081 gcgcaccgcc cccgacatgc gcaccgtgct tgccgcgcgg gcgagggccg cggccgggag
     4141 aactgccccc gctcacgggc tctatctcgt ccgagttgac tataccgaag ggagcatccc
     4201 ctctctccct taaggaactc ccccgcgaga caggtcgggc gccgtttgtt atagttccgc
     4261 acacaaccca cgttcacctt gaaaggagcc cgccatgaaa ctgctctgta cgaccttgtc
     4321 cgttctcatg ttggcagccg tactcgccac gccgcagctc agctacgccg gcgcacagca
     4381 gaacaaaatg aaaacctgca atgccgatgc aaatgccaag ggactcaccg gggagggcaa
     4441 gggtgacgcg cgaaaggcat tcatgaagga atgtctctcc gctaaaccgg agaaagccgc
     4501 tgccggtgcc tcacaacaga acaagatgac gacctgtaac aaggatgcca aggccaagaa
     4561 actggccggc gacgagcgca agaagtttat gaaggagtgc ctgtccaact agtcgtagcg
     4621 ccccggcgcg accgcacggc cgcgccgggg ctcgacagcc cgccggtcat tcacctgcac
     4681 tgtctaccac aggcctacgt tacgtatctc aacacaacgg gaatagatgg gttcgtcagc
     4741 ggaggagtga ggggatgcta ccgggggctg ttggggtctc gattttcgag cttctttcgc
     4801 aactcgatca gttccagttc gagcgcggca aatcgatcgg cggtcggatc cggcatattg
     4861 gtatggtcca tggtcgcatc ggggagccgc tccccttgcg acttgatgat cctggccggc
     4921 accccgatca ccgtcgaatt ggggggaacc gacttcaaga cgacggaatt cgcgccgatt
     4981 ttcacgttgt cgccgatcgt aatcccgccg aggatcttcg cccccgcccc gacgaccaca
     5041 tggctgccga gcgtgggatg gcgcttgccg cgctccttcc ccgttccacc aagcgtcacg
     5101 ccctgaaaca gggtgacgtt gtctccgacc tcagccgttt ctccgatcac cacccccatg
     5161 ccgtgatcga taaaaaatcg ctcaccgatc ttggcggaag gatggatctc gattcccgtc
     5221 aaccagcggg ccaattggga aatcgcgcgg ggcaggaacg gcacacgatg ccccttcaac
     5281 caatgtgcga gccgataggc caacagagcg tgaaatcccg cataggtcgc aatgacttcg
     5341 agcctgctgg tggcggcagg atcccggtca aaaatcgctt ggaggtcctg agagatagtc
     5401 ttgaacatag gtggtaccag actcgtgagc gcaccagcgg ataggctcta gcctcgctcg
     5461 atcagacaga ccgcctgcgc cgcaatcgct tcttcacgcc ctaccgcatc caatccttcg
     5521 ccgcttttca cttttacgtt taccagatcg ggatccactt caagcacccc ggcaataatc
     5581 ttctgcatgg cggccagatg cgggctcaac cggggcgcct gtgcaatgat cgtgctgtcc
     5641 acattggcca gacgatagcc cttggcgcgc agcttctgaa cgacgtcttc gagtaacttg
     5701 agactcgaaa tattcttgaa ccgctgatcc gagctcggat agtgacgccc gagatctcct
     5761 tctcccatcg ccccgagcaa cgcatcgcaa atggcatgaa ctaacgcatc ggaatcggaa
     5821 tgcccttgca gccccttgtg gtgcggaatt tcgagtccac ccagaattaa ttttcgtccc
     5881 gccaccatcg gatgaatatc ccatccacat cccacgcgtg cagccgtcat tccgcactcc
     5941 ttgccgccgt tcgtgagctg agaatcgctt ctccgatcac gagatcttcc ggcctcgtca
     6001 ccttgatatt ttctccgctc ccctcgacca ccacgaccgg ttttccgatc aattccacga
     6061 ggtgcgcgtc atccgtcgcc tgcacaccgg ccaagaaggc cttgtgatgc ccctcttcca
     6121 accagtcacg acgaaacgcc tgcggcgtct gggccaacca gagagggcgg cgatcgacgg
     6181 tccgttcgat caccccgccg gcccccacat acttcaccgt gtcgcgcatc ggcagcgcga
     6241 tgatggccgc cccatgctcg acagccgcag ccacgactct gcggatcatg tcctcggtga
     6301 gaaacggccg caccgcgtca tgaaccagga ccatctcggt ctcctgactc acctcggcca
     6361 gggcatgacg cacggaatcc tgccgctgcg ctccccccgg tacaaccttg gtcaccttgc
     6421 tgaactcacc cgtcgccacg atatgatcca ggcaatactg acgttccgac tcgggcaccg
     6481 ccagaatgat ctcgtgaatg agcggggaag cttggagcac aaggagggac tgcgcgagaa
     6541 tcggccgccc ccctaacgca agaaactgct tggggatatg gccgcccatg cgaagcccac
     6601 gaccggcggc cggaaccagg gccaccgtgc gcggccctgc cagcgacggc ttcgcgttac
     6661 gagcggacac cggcacactc acgtgagcag caaggggaca aggccaaacc taggcaatcg
     6721 tgggagctcg atgcgcctcc ccggaggacg gaggattcca ttgggccacc gctcttccgt
     6781 ctcgccaaac tacccacgcg caacctgata ctcttcgcgc tcggactctt ccttcaaacg
     6841 ggtgaagatc atgcgccccg ccgtcgtctg aagcacactg gtcacagtca catccacatt
     6901 acggccgata caacgtcgag cattgtccac gacaatcatc gtgccgtcat ccaggtatgc
     6961 gacgccctgg cccgcctctt tgccttcctt caagacgaag acccgaatgg tttcacccgg
     7021 caacacgacc ggacgcagcg cattacagag ctcattgatg ttcaacactc gcacgccctg
     7081 caattcggcg actttgttca ggtttaagtc attcgtgacg atgcgccccc cgactttctt
     7141 cgccagaacc acgagcttgg catccacttc cttcacatgg gggaaatctt cgtcgatgat
     7201 ccggacatcg atgcccggca ttttctgaat cttgttgaga atatccaacc cgcgacgccc
     7261 gcgcgccctt ttcaaagaat ccgacgagtc tgcaatgtgc tgtaattcat tcaagataaa
     7321 gtggggaacc agaaacggcc cttccagaaa ccctgtctca cagaggtcgg caacccggcc
     7381 gtcgatgatg acgctggtat ccaatacttt aaggctggca tgggaagccc cggctgccgt
     7441 cggcccctgg tccagaccgg ggaactgttc gttgccgaac cgcgcgccca tggccaaccc
     7501 cagatagggc aaccctaaaa gaaacaccaa cccgccgata tgaaacagga aggtctccac
     7561 atcgaagacc gcactcccga cccactccac caaacccgtc agaataagac caccggcgag
     7621 ccccacggca ccgccgacaa taatgccgaa tgaaagcctg cggagcgcat actcgccggc
     7681 caggatgagc ccaccggcca ccgctcccat tcccaatccc atcaacaaaa actcgcgact
     7741 tggatcctgg gcccgaagaa acagggccat gccggcaaga gcgctgagaa gaacaaatat
     7801 ggcccgtgtt accatactgt ttgcctcctc tctgtgacca accccgaacg gacgattcgg
     7861 tgcgatcacg gcctcagcgt cagataaatg gctcgcccct gccgtctcaa caggagcaat
     7921 accgcctggt ctttcggcaa attcgcggcg acatgttcat acgcctttaa cgtccccacg
     7981 gctttgcgat tcacctccag tacgagatcc ccttcacgca caccggcttc ttccgcggga
     8041 ctccccggtt taacccgcac caccaccacg ccgtgatcgc tcgacttcaa gccataccgg
     8101 ccggccagtt cggtgttcaa ctcccgcacc tcgacgtccg agagtaagcc ggcgggagcc
     8161 accgattctc cgccctcttc aaccccggct tgtgccagat tcttcggctg ctcagcaatc
     8221 gtgagttcga tggacttggg cttcttttcg cggatgagct tgaccgtcac cttcttcccc
     8281 actaccgtct gagccacggc attccgcaag tgagccggag aatccatcgg tttcccgtcg
     8341 tattcgacga tcacatcgcc gcgctcaaaa cctgctttct tcgccggact gtcgtccatg
     8401 atgtcgctga cgaggacacc cttcacgtct ttcggcacgc cgaactgcga agacaattcc
     8461 ggcgtcagct cctgaatcga gacgcccaac cagccgcgga cgactttgcc atgctggacc
     8521 agctgctcca tgatggaatg ggccatgttg ctcggaacgg caaagccgat ccccatgttc
     8581 ccgccgctct ggctatagat ggccgtgttg ataccgacca gttcaccgcg cacattcacc
     8641 agagcgccgc cggagttccc cgggttgatc gccgcatcgg tctgaatgaa gtcttcatat
     8701 tcggcgatgc cggccgcgcg accaagggcg ctgacgatcc ccagcgtcac cgtctgcgtc
     8761 aacccgaacg gattgcccac tgccagcaca aactcaccca cctccagctt gtcggaatcg
     8821 gcccaggcga cggtcggcaa tccaccggcc tcgatcttca gcacggcaac gtcggtcttg
     8881 gcatccgtgc cgaccagctt ggccttgaac tcacgcttgt cggagaggaa gaccttgatc
     8941 tcgtcggcct tgctcaccac atgattgttg gtgataatga ggccgttcgg atcgacgatg
     9001 acgccagacc ccaaccctcg ttccttgcgt tccttcggcg cctcgaaccg cttcatccat
     9061 tcttcgccga aaaatcgtct aaaaaatgga tcctcaaacg gggtgccctt cccctcctcg
     9121 ctggatccat tccgtgtcgc aaaaatgttc acgacggccg gcttcacgac tttggcgaca
     9181 tcgacgaaac tctgcccacc cgcgccgggc agcgtgggct gcaccgccgt ggagaccggg
     9241 gttgcgaccg gaggaggaac cggttccgga accgcatgtc cggtcggcaa ccagccgaga
     9301 tcggaggtca gaatgacccc gatcaatacc cccgcgatca agagcagtgc gggaaatatc
     9361 caggaccgcc tcggaggctg ctgctccgtt gggatctgaa agtcgttcat tgtgatccga
     9421 gtgggcggag gcgccggtta gggcttctct cccgcataca gtcccatgat ggtgtgcaag
     9481 aacttgacgg cctcggcttt gggacgttgg aaggaattcc ggccgatgat ggaaccgaat
     9541 cccccgccgt cacgaattgc acggacttcc tcgaacaccg tcttttcgtc gctcttcgcg
     9601 ccgccggaga agatgacgat ccggcgccca tcgaacgaac tttgcacgat atgtttgacc
     9661 cgttccgcca aggtcgagat cggcaccttt tcagcttcat agaccttctt ggcagccgcc
     9721 tgctccagat gggccgtggg caatttgacc ttgatgatat gggcgcccaa ctgcgccgca
     9781 atctgggcgg cataggccac gacgtccaac gccgtctcgc cttccttgct caatcctgac
     9841 ccccgcgggt aggaccagac cacgacggcc aatccgcagc tcttggcttc ttccgcaatc
     9901 gcccgcaact gctcgtacat cgcgttgcag tggcacgagc ccggataaat cgtgaatccc
     9961 accgccgagc agcctaggcg caaggcatcc cgcacgctgc cggtcaccga gggcatcgga
    10021 tccttctcat cgtgaagcgt atcgtggctg ttgagcttga ggatgagcgg gatttgtccg
    10081 gcgaaatggc tggcgccggc ctcaatgaat ccgagcggag ccgcataggc gttgcacccg
    10141 gcatcgagcc ccagctgaaa atggtaatgc ggactgtagc cgccggcatt gggcgcaaaa
    10201 cttctggccg gaccatgctc gaacccctgg tccaccggca ggatcaccaa tttccctgta
    10261 cccgccagct tgccgtgatt cagcagccgc gcgatgttcg tcttcgttcc cgcattgtca
    10321 ctctcgtacc agctcagaat ttcctggacc cgattgctca taatgatcct cccaaccaac
    10381 ctcagtcaca tgagtcgtta cgcacgtcct tgaatatagg cttccgcctg gctcacatcc
    10441 tcagcgctcc cgatgatcaa tggcactctc tgatggaggg ccttcggttc gacgtcgaga
    10501 atgcgcatca tccccgtggt cgcttttcct ccggcctgct ccacgaccat cgcaagcgga
    10561 tttccctcat acatcaatcg cagttttcct tccggcttat cgttctcccc gggatacaaa
    10621 tagatgccgc cacccagcag tatgcgatga acatccgcga ccaaacaccc gctgtatcgg
    10681 ccgctgtagg gacgcccggt cgctttgtcc tgtactttca gcgaatcgag atatttctgt
    10741 gtgccggaag gccaacgatg gtagttgccc tcattgaccg aatacacctt gcccttcgag
    10801 ggaattcgaa tgttctcgtt ggacaggaga tattcaccga tggacggctc gagggtaaag
    10861 ccatgcacgc cctgaccgac cgtgaacacg agcatggtgc tcgccccgta caacacatac
    10921 cctgcggcga tctgctcagt ccctttttga agcaactcat cttcgactga ctgccccgtt
    10981 ttcgatctgc ggatggagaa aatggccccc aacggcatat tgacgtcggt attggaggat
    11041 ccgtccagcg gatcgaccag gagcatatac ttggctcctt catgaaccat tttcaggggt
    11101 ttctccatct cttcagaagc caaaacacac acgagaccgc tgtgctcgaa cacacgcacg
    11161 aaggtatcat tggcaatctg atcgagcttc ttgaccacct caccttgaat attggtctcc
    11221 ccagtggtgc caaggatctt attcaatccg gctctgcgca ggtcatgcgc aatcattttt
    11281 cccacgaggc cgatctgggt cagcagaaca gaaaattctc ctgttgcttc cggatgcgcg
    11341 gcctgttgtt cgataataaa acggctcagt gtaatgggga atttcgccat gctcaggatt
    11401 cctcgtggat caaacgacga ccggtcgaaa tgaagagggc ctcggacaga gccgaagccc
    11461 tccccacgaa ctcggcgtga ttatagcggt ttccgtccgg acgagcaaac caaggcaggc
    11521 agattacggc tgcgaggtgg cttcgtaccc ctcggtcaat tcagccacaa tcgaccctac
    11581 gacaacctta gccttcattc tgagggggac acggtgctca tcggctgtga accagacgcg
    11641 aatattgccc tcattcagga aaatcccttg aaacggcatg atgacgacca ctcgcgccgt
    11701 ctgccgcttc ccccacaccc cctccactgt ttcaagggct tcgacccgca cctccatctt
    11761 gtagttcttt ttgtcgtgat gcaccgtcat cgccagcgac gcccccggga cgaacggcaa
    11821 caccttgcgc acatagtaca agcacgacat ggcgtcctgc gcatcgggag gaatcgacaa
    11881 ttcgtccgtt ttcccgtctt tcacggcagt gaccaggcct tcaccgtgcc ggaaggtata
    11941 gtcgaaatcg ttgaatcgct tcccctcgcg gcgggcaaag gtcatgtgtc tgggtaagaa
    12001 ggattcgcga tcgaccacgg ataccccgcg gttatcgacg ggataaaact tggtaacggt
    12061 cgggctcgat cgagccgtca tggcaaaagt gagctgcggc cggggcccct cgaccgggcc
    12121 ctcctgaacg atcatggtcg cgattcccgc gcgcatgccc aaccaggtca cgtcgtagga
    12181 caatcgctcg ccgtttgcaa agggcaacgc ggcaggagtg aggatctgcc cgtccggtgt
    12241 tgcataagag agcaacgggg cagaggcaac gactgtagcc aggatcgtga cggccaggaa
    12301 caggctgaga cggagggagg cagagtagcg ggacacgcta cgtcgacagc acacgtcgtg
    12361 cctgcgcgag ctcggtttca atcgtcgcgc gaatcacatc cagccgggcc ggcgacgggg
    12421 cctcaaagcg gagcaccaac gcaggctggg tattggaggc tcgaatcaag ccccacccgt
    12481 cttcgaaaat cgcgcgcacc ccgtcgatcg tcaccaactc gcgcatgcga agattcgacg
    12541 caccgacggg ctggccctca gcgagatagg cggccaattg cgtgcgcacc tgatcgacca
    12601 gttgaaactt cagcgtatcc gggcagtcga cccggatctc cggcgtcacc gtggtctggg
    12661 gaaggtcggc gaccaaactg gagagcgacc gcttggtctt ggccagaatc tcaaccaacc
    12721 ggcaggacgc atagatcgca tcatcgaaac cgaaataccg atcggcaaaa aacatatggc
    12781 ccgacatttc accggccagg accgcggact cggctttcag cttggccttc atcagggaat
    12841 gaccggtctt ccacatgatg ggacgcccgc ccttggcggc aatatcgtcg tacaggcatt
    12901 gcgaagcctt gacctcggag ataaaggtgg tgcccgggcg ctgggtcagg atatcccgtg
    12961 catagacaat catgaggcga tcaccccaaa
//
LOCUS       DAORRQ010000084        12826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_238_length_12826_cov_66.765249, whole genome shotgun sequence.
ACCESSION   DAORRQ010000084 DAORRQ010000000
VERSION     DAORRQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12826
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238_length_12826_cov_66.765249"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK224_18185"
     CDS             <1..220
                     /locus_tag="PK224_18185"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRB17731.1"
                     /translation="GLLTLGFEALPSETNFLYFDVGRDGREVFDALLRKGIIVRHIDG
                     RMLRVTIGLPEENQLFLSALADVTRAAR"
     gene            239..1252
                     /gene="aroF"
                     /locus_tag="PK224_18190"
     CDS             239..1252
                     /gene="aroF"
                     /locus_tag="PK224_18190"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRB17732.1"
                     /translation="MIIVLKPDASEREVDHIIDRLRELGLKSHISAGQERTIIGVIGD
                     DRILHNQPLTALPGVESVLPILAPWKLVSREFKKENTIIDVNGVKIGDKKITIMAGPC
                     AVERLELTVGIAHEVKSAGATVLRGGAYKPRTSPYSFQGLGREGLDYLVEAKKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNRNVMLCERGIRTFETQYRNTLDMAAIPTLKSLSHLPVIVDPSHATGK
                     WDLVAPMSKAAIAAGADGLLIEVHSNPECALCDGEESIRPSKFKELMGDLRRIAAAVD
                     RTI"
     gene            1312..2208
                     /locus_tag="PK224_18195"
     CDS             1312..2208
                     /locus_tag="PK224_18195"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRB17733.1"
                     /translation="MMTPHFKQVAIVGVGLIGGSLGMILRRQKLADSVVGIGRRVENL
                     KTAVEVGAIDRYVSDPREGVEGADFVLLATPVDTYERHLREWAGCLKPGAIVSDVGSV
                     KGELVTRAEALLPPSVRFVGAHPIAGKEKTGVAAGSETLFSGARCILTPTAKTDPEAL
                     QTVRVLWELAGSIVLDMDPFLHDKILGAVSHLPHVAAFALMTALADVRDHGLPELDLA
                     SHSGGGLRDTTRIAASSPEMWRDIFLWNRDNVVSLIETYERHLGELKRLIAAGDAAGI
                     EKQLDQAKYEREQLTPRTSGKS"
     gene            2215..3543
                     /gene="aroA"
                     /locus_tag="PK224_18200"
     CDS             2215..3543
                     /gene="aroA"
                     /locus_tag="PK224_18200"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRB17734.1"
                     /translation="MASLTITPGRPLKGTIAVPGDKSVTHRAIILTALAEGLSQVTDY
                     CRGEDCLNTMRAFQSLGVRIEETPERLTVHGKGMWGLTEPFGPIDCGNSGTGIRLMAG
                     LLAGQDFFTVLTGDESIRRRPMGRVVKPLRAMGATIAGRKGGELAPLAITGTRLKGMS
                     YESPVASAQIKSSLLFASLYADGLTTISEPRLSRDHTERMFAYFGIPFHRDGCTVRIE
                     GRPSIRWSGKTVVVPGDLSAAAFFIVGASIVPDSDVTVLSVGMNPTRTGLLDILRQMG
                     AHIEVLNPREEAGEPVADLRVRSMPLRGVQIGPEQIPQTIDEFPILCVAAAVADGETV
                     ITGAEELRVKESDRIATMAAELRAMGARIEERPDGMVIQGLGRKGANGTLTGATCASH
                     GDHRVAMSVAIGALTAAQPTQIQDTVCIETSFPNFDRKLLELLTDSGKRL"
     gene            3556..4254
                     /gene="cmk"
                     /locus_tag="PK224_18205"
     CDS             3556..4254
                     /gene="cmk"
                     /locus_tag="PK224_18205"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRB17735.1"
                     /translation="MGEPGTSGCGKRGLIIAIDGPAGVGKSTVARLLALRLGYLYLDT
                     GALYRAIAWKVRDAGLSPENQFAITALLPKTTLHMACGPEQSHVLLDGRDISGELRTP
                     AVTALASMVSAIPAVREWLLPVQRQIGAEGCVVAEGRDIGTRVFPEADVKFFLEADAE
                     VRATRRHRELVAAGHSVQFDQTKRDMTGRDDRDRSRTVAPLIPAPDAERIDTSSMPAE
                     AVVEHMLAVITARL"
     gene            4251..4946
                     /locus_tag="PK224_18210"
     CDS             4251..4946
                     /locus_tag="PK224_18210"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRB17736.1"
                     /translation="MSSALYGLLWVISRTIGWLCFRYRTVGTVPRQGGFLIASNHASY
                     LDIPLLGCGIPRRVWYMGRHDLFPMPLLNGLLQALGWIPLRVGRLDRDAFSKAVSLIQ
                     EGKAVAIFPEGGRTMTGALKPGKPGIGVIVSQTGCQVVPAHIGGTFEVLPPGAKWPRF
                     RRVTVAYGEPLDFSADAARLEGKAFYQHVSRTVMAKIAELGQVPIPGDRPANAGQPHD
                     SAATPTAKSCNAE"
     gene            5004..6719
                     /locus_tag="PK224_18215"
     CDS             5004..6719
                     /locus_tag="PK224_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRB17737.1"
                     /translation="MSTVTPQSEPKLDRDALAAMYEETFRNFEEGTITEGMVVAIGKD
                     KVVVDIGYKSEGMIPADQFSHEELAQLKVGDRLQVYLEECEDADGNLVLSKEKADKMK
                     IWEELETLHKEEKSIEGKIISRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGKTF
                     PLKIIKINHRRGNVVVSRRVLLEETRDRRRQTTLSTLKEGQLIQGTVKNITDYGAFID
                     LGGIDGLLHITDMSWGRVGHPSELFQVSDKVEVTVLKYDRETGRISLGLKQKSADPWT
                     GVAAKYPVGTRVRGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSRVVSVGD
                     QVEAAVLNIDPGSRKISLGMKQTAPNPWDMIEAKYPAGTRIEGKVKSLTDFGAFVGLE
                     EGIDGLIHISDMSWTKHIKHPSELFKKGQKVDAVVIRIDKEKERLSLGYKQLSRDPWE
                     EQIPNKYRVGDSITGKVSKIADFGLFIELDGDVEGLIHISEVGLDTNVRMEEKFKPGD
                     DVTAKIIKVDRDERKIALSLRDHQMDSDRRQVDEFHASQGGIDQTLGRAAKQSRKRNQ
                     SDSEA"
     gene            6735..7634
                     /gene="sppA"
                     /locus_tag="PK224_18220"
     CDS             6735..7634
                     /gene="sppA"
                     /locus_tag="PK224_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRB17738.1"
                     /translation="MGPQADTAVRPQRSLLRRIFWAIVIGGGALILLNALLPDLDFSS
                     QDRVALIRIEGVILDAQATISELKQYSENPLVKAIVLRIDSPGGGVVPSQEIHDAVKR
                     VKNKSNKAVIASMGTVAASGGYYIAAATDRIIANPGTLTGSIGVIMEMANFEGLMKKV
                     GVEGVVIKSGRFKDVGSPLRKMSDEERKLLQSVMDDVHHQFIQAVADGRSLEVSDVEP
                     LADGRIYTGRQAKEARLVDELGDLDDAIHIAADIAGMEGEPKVVEPRKRFSFRDIIES
                     RWASVFPKLELNTGVKLKYLMAF"
     gene            7708..7986
                     /locus_tag="PK224_18225"
     CDS             7708..7986
                     /locus_tag="PK224_18225"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRB17739.1"
                     /translation="MTKAQIIERVSEQVTTLTKRQAEIVVNTIFDCIRDSLRNGNKTE
                     IRGFGSFRLRARRMKEGRNPKTGATVAVPAKRVPFFKAGKELKELLNK"
     gene            8000..8995
                     /locus_tag="PK224_18230"
     CDS             8000..8995
                     /locus_tag="PK224_18230"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HRB17740.1"
                     /translation="MAESSNITAPEVRWSDGALKRMERAPMFLRGMVRRLAEKKAREL
                     GYPEITEEILDQFKGQMMGTMGGEGGMAEAADQMAKGQLPWTAAAKERLAAVPEFMRG
                     MIKQIADEIARKGGHMEVNVDLFEKVEALGEIREREAAPLEWSEGALALLQQKIKESP
                     PIAMDFVSDMIRHDTEELAREKGFTRIDEQIAVQLWEAPQERVAWSDEAWKRLQTSPD
                     FVRSGIRKAAERRARKLGLKEVDSEHLTTFRNQAMMKAVKRIRSFGYNELTFDAFDTA
                     LQKTKRLQGNDQAEKRLQEIRSHFSDPEVKKPEGGTLGADLMGRFRKFLKGEGSL"
     gene            complement(9278..9598)
                     /locus_tag="PK224_18235"
     CDS             complement(9278..9598)
                     /locus_tag="PK224_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17741.1"
                     /translation="MSKTANIPLSMYGVAEVLKWCIDRNNGRVSGVDTDGFKKMQALL
                     AEKPTSNDYLALDQFWKKQVMLELTEDEVSTIDRCLYDIPNFDNEPLPQIRHKFWPDA
                     VGAH"
     gene            9845..11041
                     /locus_tag="PK224_18240"
     CDS             9845..11041
                     /locus_tag="PK224_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRB17742.1"
                     /translation="MTADRRGIKIRSFFGVGPMLLAGLIMLSACSKADPYVPPDPFYY
                     FASYPVGKNPTTVTTADFNRDQITDLITTNISSNTISLLFGNGDGTFRDQVQVKVCQE
                     PRSLAINDFNHDDQLDVVLACSGSDQVSILFGRGNGKFEDGPQYPVHRTPVSVSSEDV
                     NGDGHPDLAVALRNDKVKIFLGSAGGTFRPGVQYEYGDTPTSVALKDLNQDGKPDLVV
                     TNGGPMLSAVSVWIGNGDGTFRDPKDYKTGRRPLGVSFADFNNDSLPDLLVINGEGDS
                     FTTLLGNGDATFQPGKDSGADASPNFGAAHDFDGDHIADVAIVNLQSTDLSILFGRGD
                     GTFHYPPRNYRTRNGPFAVASYRVSADNMEEPGLVTADNGAGSVSVFLHHGRKGQASA
                     AGRAGE"
     gene            11210..11383
                     /locus_tag="PK224_18245"
     CDS             11210..11383
                     /locus_tag="PK224_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17743.1"
                     /translation="MGCLTTLAVLMVQGGVRDLVSGTNPAGSSGAILSFSTTVFGGGL
                     LAGCLALILNRLR"
     gene            11463..11651
                     /locus_tag="PK224_18250"
     CDS             11463..11651
                     /locus_tag="PK224_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17744.1"
                     /translation="MHCLKCKGFMMVERHYTLMNRRLYARCLNCGFWIDLADLLRFFH
                     KVISSSLRGTKVRETYTL"
     gene            11697..12626
                     /locus_tag="PK224_18255"
     CDS             11697..12626
                     /locus_tag="PK224_18255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRB17745.1"
                     /translation="MNRLRRTLSIGAFGILALLFGLWSGQALALDSDVDDEVITVPFD
                     PRPVPSAKQANHSLRWRHVPAHSILLKELKTGTTLYQFESEKRLSPASLTKIMSALVI
                     LEYGHLDDEVTVSPKAARAHKTHLRLRTGQIFRLEDLLKAMLIVSANDACLAASEHVG
                     GDEARFVDLMNAKAAALQLHNTHFSNACGFDAPEHYSTAEDLAKLSEIALHHPVFKEL
                     VREEREIIMPINEHRAYVLRTTNRLLGRIPGVQGVKTGFTSKAGRCLIAKVSQDGSDL
                     LLVILNSKRRWNTATSLINYGLRLSDSRAGLAR"
BASE COUNT         2669 a         3415 c         4111 g         2631 t
ORIGIN      
        1 aggcttgttg accttgggct tcgaggcctt gcccagtgaa acgaattttc tctatttcga
       61 tgtcgggaga gatggtcgcg aggtattcga cgcgctgttg cggaagggca tcatcgtccg
      121 ccacatcgac ggccgaatgc tgcgggtgac catcggcctt ccggaagaaa atcaactgtt
      181 cctgagcgcg cttgcggacg tgacgcgcgc cgctcgataa ggaaagcgag acgagatcat
      241 gattattgtc ttgaagcctg acgcgtcgga gcgggaagtc gatcacatta tcgaccggct
      301 tcgcgagttg ggactgaagt cgcatatttc tgccggccag gaacggacca ttatcggggt
      361 gatcggggac gatcgtatcc tgcacaatca gccgttgact gcattgcccg gggtcgaaag
      421 tgtcctgccg attttggctc cctggaagct ggtcagccgg gagttcaaga aagagaacac
      481 gatcattgac gtgaacggcg tcaaaatcgg cgataagaaa atcacgatca tggccggtcc
      541 ctgcgcggtc gaacggttgg aactgaccgt cggtattgcc cacgaggtca agtcggccgg
      601 cgcgaccgtc cttcgtggcg gcgcgtacaa accgcgcacc tcgccctatt cttttcaagg
      661 attggggcgt gaaggcctgg actatttggt cgaagccaaa aaacagaccg gcctgccggt
      721 ggtgagcgag atcctggaca cccgcgatat cgagctgttt cttgagaagg ccgacatcat
      781 ccagatcggc gcccggaaca tgcagaactt cgagttgctc aaggaagttg gggcctacga
      841 taagccggtg ctgttgaagc gcggtctctc ggccacgatc aaggaattcc tcctctccgc
      901 tgaatacatc atgtcccgcg gcaaccggaa tgtcatgtta tgcgagcgcg gcatccgcac
      961 gtttgaaacg cagtatcgaa ataccttgga tatggccgcc attccgaccc tcaagagtct
     1021 gtcgcatttg ccggtcatcg tggatcccag tcatgcgacg ggcaaatggg atttggtggc
     1081 gcccatgtcg aaagcggcga tcgcagccgg cgccgacgga cttttaatcg aagtgcattc
     1141 caatccagaa tgtgccctct gcgatggcga agagtctatc cggccctcga agttcaagga
     1201 gttgatgggg gatttgcgga gaattgctgc ggcagtcgac cggacgatct gatcaccgtt
     1261 acgaacgtga tcgtcgaccg tgcgggtgag ttgaagatac gcactcattc aatgatgaca
     1321 ccacatttca aacaggtggc gattgtcgga gtcgggctga tcggcggctc gctggggatg
     1381 atcctgcgcc gtcaaaagct ggcggattcc gttgtcggta tcgggcgacg cgttgaaaat
     1441 ctcaagacag ccgtcgaagt gggcgccatc gatcggtatg tatcggaccc ccgcgaaggc
     1501 gtcgaagggg cggactttgt cctgctcgcg actccggtgg atacctacga acggcatttg
     1561 cgggaatggg ccggttgcct gaagccgggc gcgattgtca gtgacgtggg cagcgtcaag
     1621 ggtgagctgg tgacacgcgc ggaggccttg ctgccgccgt cggtgcggtt cgtcggtgcg
     1681 caccccattg ccggcaagga aaagaccggg gtggccgcag gatcagagac tcttttctca
     1741 ggggcgcgtt gcattctgac tccgacggcc aagaccgatc cggaggcgct tcaaacggtt
     1801 cgtgttctct gggaactcgc gggttcgatt gtgttggata tggatccctt tctgcacgac
     1861 aagattctcg gtgccgtcag tcacctgccg catgtcgccg cgtttgcatt gatgacggcg
     1921 ctggccgacg tgcgtgacca tggtttgccg gaactcgatc tggccagcca ttccggcggc
     1981 ggattacgcg ataccacgcg aatcgcggcc agttccccgg agatgtggcg agacattttt
     2041 ttgtggaacc gggacaatgt ggtgtcgttg atcgagacgt acgagcggca tctcggcgaa
     2101 ctcaaacggt tgattgcggc cggtgacgcc gctgggatcg agaagcaact ggatcaggcc
     2161 aagtatgaac gggaacaact cacccctcgc acatcgggga agagctaggc tgccatggca
     2221 tccttaacta ttacgccggg ccgtccgctg aagggcacga ttgcggttcc tggcgacaag
     2281 tccgttaccc atcgggcgat tatcctgacg gccctcgccg aggggctgag ccaggtcacc
     2341 gactattgtc gcggggaaga ttgtttgaat accatgcgcg cgttccagtc gctgggcgtg
     2401 cgaatcgaag agacgccgga gcggttgacg gtccatggca aaggcatgtg gggtctcacg
     2461 gagccgttcg gcccgatcga ttgcggcaat tccggaaccg gcattcgtct gatggcgggg
     2521 ttgttggcag gccaggattt cttcacggtc ctgaccggag acgaatcgat cagacgtcgt
     2581 cccatgggac gtgtcgtgaa acccttgcga gccatggggg cgacgattgc cggacgaaag
     2641 ggcggggaac tggcgccttt ggcgatcacc gggacccggc tgaaaggcat gtcgtacgag
     2701 tccccggtcg cgagcgctca gatcaaatcg tcgctgctct tcgcctcgct gtatgccgat
     2761 ggcctgacga ccatttcaga gccacggctt tcccgtgacc ataccgagcg tatgtttgcc
     2821 tacttcggaa ttccttttca ccgtgacggg tgtacggttc ggatcgaagg gcggccctcc
     2881 atacgctgga gtggaaaaac cgtggtggtg ccgggtgacc tgtccgcagc cgcatttttt
     2941 atcgtcggcg cctcgattgt gccggattcc gatgtgaccg tgctgtcggt cggcatgaat
     3001 ccaacccgga ccggtctatt agacattctg cgccagatgg gcgcgcacat cgaggtgctc
     3061 aatcctcgtg aagaggccgg ggagccggtc gcggacctgc gcgtacggtc gatgccgctg
     3121 cggggagttc agatcgggcc ggaacagatt ccgcagacga tcgatgagtt cccgattttg
     3181 tgtgtcgcgg cggccgtggc cgacggtgaa acagtaatca ccggagctga agagctgcga
     3241 gtcaaagaaa gcgaccgtat cgcgacgatg gcagctgaac tccgggcgat gggcgcgcgg
     3301 atcgaagaac ggccggatgg catggtgata caggggctgg ggcgcaaggg tgcgaacggc
     3361 acgctcacag gggcgacttg cgcgagtcac ggcgaccacc gcgtggccat gtccgtcgcg
     3421 atcggtgcgc tgaccgctgc ccagccgact cagattcagg acacggtttg catcgagact
     3481 tcgttcccga acttcgatcg taagctcttg gaactgttga ctgattctgg gaaacgtcta
     3541 tagtgcgagg attacgtggg tgaaccgggc acatccggct gcgggaagcg cgggttgatc
     3601 atcgccattg atggtccggc aggggtcggc aagagcacgg tcgccagact cctggcgttg
     3661 cggcttggat acctctatct ggatacaggc gccttgtatc gcgccattgc gtggaaagtg
     3721 cgagacgcgg gattgagccc tgagaatcag ttcgccatca ccgcgctgct tcccaagacg
     3781 acgctgcata tggcctgtgg tcccgagcaa tcgcacgtgt tgctggatgg tcgcgacatc
     3841 agcggtgaat tgcgcacgcc cgcggtgacg gcgctggcgt ccatggtttc ggcgattccg
     3901 gctgtccgcg aatggctgct gccggtgcaa cggcagatcg gcgccgaggg gtgcgttgtg
     3961 gccgaggggc gcgacatcgg aacccgggtg tttccggaag ccgatgtgaa gtttttcctc
     4021 gaggccgatg cggaggtccg ggcgacgcga cggcatcgtg agttagtggc ggcgggtcat
     4081 tcggttcaat tcgatcagac gaaacgcgat atgaccgggc gggatgatcg cgatcgttct
     4141 cgaaccgtgg cgccgctgat tcctgcgccc gatgctgaac ggatcgatac gtcgagtatg
     4201 ccggcggagg cagtcgttga gcacatgctg gccgtgatca cggcgagatt gtgagctcgg
     4261 cactgtatgg cctgttgtgg gtcatttctc gcaccatcgg gtggctgtgc ttccggtatc
     4321 gcaccgtcgg gactgtgccg cggcaaggcg ggttcctgat cgcctcgaat catgccagtt
     4381 atctggatat cccgctgctc gggtgcggga ttccccgccg ggtctggtat atggggcggc
     4441 acgatttgtt tccgatgccg ttgctcaacg gcctgctgca ggccctgggg tggattccat
     4501 tacgggtcgg tcgtctcgat cgcgacgcct tcagcaaagc ggtctcgttg atacaggagg
     4561 ggaaggccgt cgccattttt cccgaaggtg ggcgtacgat gaccggcgcg ctgaagcccg
     4621 gcaagccggg gatcggggtg atcgtgtcgc agacgggctg tcaggttgtg ccggcgcata
     4681 tcggcggcac gttcgaggtg ctgccgcccg gcgccaagtg gccgagattt cgtcgcgtga
     4741 cggtggccta cggagagccg ctggattttt ccgcggatgc cgcgcggttg gaaggaaaag
     4801 cgttttatca acatgtcagt cggacggtga tggcgaagat cgcagagctc ggacaggttc
     4861 cgattccggg ggacagaccg gccaacgccg ggcagcccca cgattccgcc gcaacaccaa
     4921 cagccaagtc ttgcaacgct gagtaagact cggtttttct cactatcagc acccggcacg
     4981 agccggagga cgaggacctt ttcatgagta ctgtcacacc gcaaagtgaa cccaagctgg
     5041 atcgcgacgc cttggcggcg atgtatgagg aaaccttccg caattttgag gaaggcacca
     5101 tcaccgaggg catggtcgtg gccatcggca aggacaaagt cgtggtcgat atcggctaca
     5161 agtcggaagg catgattcca gccgaccagt tctcgcatga agaattggcc cagttgaagg
     5221 tcggggatcg cctccaggtc tatcttgaag agtgtgaaga cgcggacggc aatctcgtgc
     5281 tctccaaaga aaaagccgac aagatgaaaa tctgggagga attggagacg ctgcataaag
     5341 aagaaaagag catcgaaggc aagatcattt cccgtatcaa gggcggcatg atggtcgata
     5401 tcggcgtcaa ggcgttcttg cccggctccc agatcgatct gcatccggtc cgcgatctcg
     5461 atgggctggt cgggaagaca ttccccctca agatcatcaa gatcaaccat cgccgtggca
     5521 atgtggtcgt ttcccgacgg gtcttgttgg aagagacgcg cgaccgtcgc cgtcagacga
     5581 cgttgtcgac cttgaaggaa ggtcagttga ttcaggggac ggtgaagaac atcaccgatt
     5641 acggagcgtt tatcgacctc ggcggcatcg acggattgct gcacatcacg gatatgtcct
     5701 ggggtcgtgt ggggcatccc tccgaattgt tccaggtcag cgacaaggtc gaagtcaccg
     5761 tgctgaagta cgatcgtgaa accggccgta tttccttggg cctcaagcag aagtcggcag
     5821 atccctggac cggtgtggcg gcaaagtacc cggtgggcac gcgcgtccgt ggtcgggtgg
     5881 tgagcttgac cgactatggc gccttcgttg agttggagcc cggcgtggaa ggattggtcc
     5941 acgtgtccga gatgtcctgg acgcacgaag tgcgacatcc gtcgcgcgtc gtctcggtcg
     6001 gcgatcaggt ggaagccgcc gtgttgaaca tcgatccggg aagccggaag atttcgctcg
     6061 gcatgaagca gacggcgccg aatccgtggg acatgatcga ggcgaagtac ccggctggaa
     6121 cgcggatcga aggaaaggtg aagagcctga ccgatttcgg cgcgttcgtc ggattggaag
     6181 aaggtatcga cgggctgatc catatttccg acatgtcctg gacgaagcac atcaagcatc
     6241 cgtcggagct cttcaagaag ggccagaagg tggatgcggt ggtcattcgt atcgacaagg
     6301 aaaaggagcg cctctcgctg gggtataagc aattgtcacg cgatccgtgg gaagagcaga
     6361 ttcccaacaa gtatcgggtc ggcgatagca ttaccggcaa ggtcagcaag atcgccgatt
     6421 tcggactctt catcgagctc gatggcgatg tcgagggtct gattcatatc agcgaagtcg
     6481 gactcgatac gaacgttcgc atggaagaga agttcaagcc gggtgacgac gtcacggcga
     6541 agatcatcaa ggtggatcgc gatgagcgga aaattgcgct cagcttgcgc gatcaccaga
     6601 tggattccga tcggcgccag gtcgatgagt tccacgcctc tcagggcggg atcgatcaga
     6661 ccctgggtcg cgcggccaag caaagccgga agcggaacca gagcgattcc gaagcctagg
     6721 tgacgggaga ccgaatgggg ccacaagcag acacggcggt gagaccacag cggagcctgc
     6781 tgcgacgtat cttctgggcc atcgtgatcg ggggaggagc gttgattctc ttgaacgcgc
     6841 tcctcccaga cctcgatttc tcaagccagg atcgtgtagc cctcattcgc atcgagggcg
     6901 tgatccttga tgcacaagcg accatcagcg agttgaagca atacagcgag aacccgctgg
     6961 tcaaggcgat cgtactgcgc atcgacagtc ccggcggcgg cgtcgtgccg tctcaggaaa
     7021 ttcacgatgc ggtgaagcgg gtaaaaaaca agagcaataa ggcggtgatc gcctccatgg
     7081 ggacggttgc tgcttccgga ggttattaca tcgcagcggc cacggaccgt atcattgcca
     7141 atcccggcac gttgaccggg agtatcggcg tgatcatgga aatggccaac ttcgaaggcc
     7201 tgatgaaaaa ggtcggggtc gaaggggtgg tcatcaagag cgggcgattc aaagacgtcg
     7261 ggtccccgtt gcggaagatg agcgatgagg aacgcaagct cctgcagtcg gtgatggacg
     7321 atgtccatca ccagttcatt caagcggtgg ccgacgggcg gtcgttggag gtgtcggatg
     7381 tggagccgct ggcggatgga cggatttaca ccggacgcca ggccaaagag gcgaggctgg
     7441 tcgacgaact gggcgatctg gacgatgcca tccacatcgc ggcggacatt gccggtatgg
     7501 agggtgagcc gaaagtggtc gagcctcgca agcggttttc atttcgggat atcatcgagt
     7561 cacgttgggc gtcggtgttt ccgaagctgg agttgaatac cggcgtcaaa ctgaaatacc
     7621 tgatggcgtt ctgaacggcc ggagcgcatc ggtaaggcaa cgcagcaatt catccgtgcg
     7681 taagccccac ccacgaggag agggacgatg accaaagcgc agattatcga gcgggtatcg
     7741 gagcaggtca cgacattgac gaagcggcag gcggagatcg tggtgaacac gatattcgat
     7801 tgtattcgcg actcgctgcg gaacgggaat aagacggaga tccgagggtt cgggagtttt
     7861 cgtctccgcg cccggcggat gaaggaaggt cgtaatccga aaaccggtgc gaccgttgcg
     7921 gtgccggcca agcgtgtgcc attcttcaag gcaggcaagg aactgaagga attactcaat
     7981 aagtaacagg agtgataccg tggcagagtc ctcaaatata acagccccag aggttcggtg
     8041 gagtgacggc gcgctcaagc gaatggagcg cgctcccatg tttttgcgag gaatggtccg
     8101 ccgcctggct gagaaaaaag cgcgagagtt gggctatccc gaaatcaccg aagaaatcct
     8161 cgatcagttc aaaggtcaga tgatggggac catgggcggt gagggcggca tggctgaggc
     8221 cgccgatcaa atggccaagg gacagctgcc ttggaccgcg gccgcgaagg aacgcctggc
     8281 ggccgtgccg gagttcatgc gcgggatgat caagcaaatc gccgacgaga tcgcccgcaa
     8341 aggcggccat atggaggtca atgtcgatct ctttgagaag gtcgaggctt tgggagagat
     8401 tcgagagcgg gaggcggctc cactggaatg gtcagagggt gcgttggcgc tcttgcagca
     8461 gaagatcaag gagtctccgc cgattgccat ggattttgtg agcgacatga tccggcatga
     8521 taccgaagag ttggcgcgag agaagggctt caccaggatc gatgagcaga tcgcggtgca
     8581 gttgtgggag gctccgcagg aacgtgtcgc ctggagcgac gaagcgtgga agcggttgca
     8641 aacctcaccg gattttgtgc gcagcgggat ccgcaaggcc gctgaacgtc gagcccgcaa
     8701 gttgggtctg aaagaggtcg actccgagca cctcacgaca tttcgcaatc aagcgatgat
     8761 gaaggcggtg aagcgcatcc ggtcgttcgg ctataacgaa ctcacgttcg atgccttcga
     8821 caccgcgctt cagaaaacca agcgattgca gggaaatgac caggcggaga aacgcctgca
     8881 ggaaatccgt tcgcacttca gtgatcccga ggttaaaaag ccggaaggcg ggacccttgg
     8941 cgccgacctc atgggtcgat tccggaagtt tctcaaaggc gaggggtcgc tctaaagcag
     9001 gaagatcaat ctgtttgggt ttcccagacc ctgtcgatcc ggggcccgtt ctgcctcatc
     9061 gcgtggctgg acgggcctat tcccccgcca acaatcaaat caacatgctt gaggctatcg
     9121 caggatggcc gcgactacgc acagtctcct tcgggaggga acgtatgagt ggagcgttgg
     9181 gcgcgtgatc tcaaacagca caatcccaca gcaacccaca gagcatgacg atctgtgggt
     9241 tgccggaaga tggtggtcga ttgcggatag ccggggctta gtgagctccg acggcgtcag
     9301 gccagaactt atgtcgaatc tgcgggagcg gttcgttgtc aaagtttgga atatcgtaga
     9361 gacagcggtc gatggtcgat acctcgtctt ccgtcagttc caacatgact tgcttcttcc
     9421 agaactggtc cagtgccaag taatcattcg acgtcggctt ctccgccagg agcgcctgca
     9481 ttttcttaaa tccgtccgta tcgacaccgc tgacgcggcc gttattgcga tcgatacacc
     9541 acttcaacac ttctgccaca ccgtacatcg acaaaggaat attggctgtc ttgctcatgg
     9601 gcacctcctc cacacttggc ccgcattgta gcctaaggct ttccaagttt tcaaaggggc
     9661 gaaaggccta gatgcttgag cccattcggc aagtagtcgt gttcgcccgg gcggcatgtt
     9721 ctcggagcgg gccattcgtt gcgaagcccg aggggcctct gtatactccg cccaacggat
     9781 gagaaccggg agcggcgaag catggacttg gccggaatgt catgcctttt tcatcgagat
     9841 ggatatgacc gccgaccgtc ggggcatcaa gattcggtcg ttcttcggtg ttggaccgat
     9901 gttattggcc gggttgatca tgctttccgc ctgttcgaag gccgatccct acgttccgcc
     9961 cgaccctttc tactactttg ccagttaccc ggtcggtaag aatccgacca ccgtcaccac
    10021 agccgacttc aatcgcgacc aaatcacgga ccttatcacc accaacatat cgagcaatac
    10081 catttcgctg ctgttcggca acggcgacgg cacgtttcga gatcaggttc aggtgaaggt
    10141 gtgtcaggag ccccgctccc tggccatcaa cgactttaat cacgatgacc aactcgatgt
    10201 ggtgcttgcc tgctcgggga gtgatcaggt ctcgatcctg ttcggacgag gcaacgggaa
    10261 gttcgaagat gggccgcagt atccggtcca tcgtacgccg gtctctgtgt ccagcgagga
    10321 tgtgaacggt gacggtcatc ccgatctggc ggtggcgctg cgcaacgaca aagtgaagat
    10381 ttttctcggg agtgccggcg gaacctttcg tcccggcgtt caatatgaat acggtgacac
    10441 acccacgtcg gtggcattga aagatctcaa ccaggacggc aaaccggatt tggtggtcac
    10501 gaatggcgga ccgatgttaa gtgccgtatc tgtgtggatc ggcaatggcg atgggacatt
    10561 tcgggatccc aaagactata agaccgggcg ccggccgctt ggcgtcagct tcgcggattt
    10621 taacaacgat tctctgcctg atctgttagt catcaacgga gaaggggaca gcttcaccac
    10681 attgttgggc aatggggatg ccaccttcca gcccggtaag gattccggcg ccgatgccag
    10741 cccgaatttc ggggcggctc acgattttga cggcgaccat attgcggatg tggccatcgt
    10801 gaatctgcaa tcgaccgatc tctccattct cttcgggcga ggcgacggca cgtttcatta
    10861 tccgcctcgc aattaccgta cgaggaacgg accctttgcc gtggcgagtt accgtgtctc
    10921 tgccgataac atggaagagc ctggtctggt gacggcggat aacggcgcgg gtagtgtgtc
    10981 ggtgttcctg catcacgggc gaaaggggca agcttcggcg gccggtcgcg cgggcgaata
    11041 gcagcaggat tcaggtttgc gccggttcat ccgaagagga gataggcagt ttgcttgaca
    11101 cctcaatagg gctcggatag agttcgaaaa ggttgccgga caaggatccg cgccatgttc
    11161 agctactaga ccgcgggggc tcgtgcgatc gtttctgtgg tggttttgca tgggctgcct
    11221 gacgacgctg gcagtcttga tggttcaagg tggggtgcgc gatctggtct cagggaccaa
    11281 cccggcggga agttcgggcg cgatcctctc ctttagcacc acagtcttcg ggggcgggtt
    11341 gttggccggc tgcctcgccc tgatcctcaa tcgactccgc taaatacagt gacgacgtcg
    11401 gtatcggcct gagctgcgct gggccgacca cgaaaccgat tcgaaggggc aagggttggg
    11461 cgatgcactg cctgaaatgc aaaggattca tgatggtgga gcggcattac acgctcatga
    11521 atcggcgtct ctatgcccgt tgcctgaatt gcggattctg gatcgatctc gccgatttgc
    11581 tccgattctt tcataaggtg atctccagta gcctgcgtgg tacgaaggtg cgagaaacct
    11641 ataccctgtg aacgtcagac ttcgtctgtg cgtcacaaga aagtgcgacc ctctccatga
    11701 atcgtttacg tcgaacgctt tcgatcggtg ccttcggaat cctggctctg ttgttcggcc
    11761 tctggagcgg tcaggcgctg gccctcgaca gcgacgtaga cgatgaagtc attacggtgc
    11821 ccttcgatcc gcggccggtt ccgtccgcca agcaagcgaa tcatagtctc cggtggcggc
    11881 atgttccggc gcatagcatt ctgctcaagg aactcaagac gggaaccacc ctctaccaat
    11941 ttgaaagtga aaagcggttg tcgccggcca gtctgaccaa gatcatgtcg gccctggtca
    12001 tcctggagta tggccatctg gatgacgagg tgacggtcag ccccaaggcc gcgcgcgcac
    12061 acaagacgca cttgcgcttg cggaccggtc agatttttcg gctggaagat ttgttgaagg
    12121 ccatgttgat cgtgtctgcg aatgacgctt gcctggcggc cagtgagcat gtcggcgggg
    12181 atgaggccag gtttgtcgat ctcatgaatg ccaaggccgc ggcgcttcag ttacacaata
    12241 cccatttcag caacgcgtgc ggtttcgatg cgccggaaca ttattcgacg gcggaagatc
    12301 tggcgaaact gagcgagatc gccctgcacc atcccgtatt taaggaattg gtgcgtgaag
    12361 agcgggagat catcatgccg atcaatgagc atcgggccta cgtgttgcgc acgaccaacc
    12421 gtctcctggg tcgtattccg ggagtgcagg gcgtcaagac gggcttcacg tccaaagccg
    12481 gccgatgcct catcgcgaaa gtctcccaag acggcagcga cttgctgctg gtcattctca
    12541 attccaagcg tcgctggaac acggcgacga gcctcatcaa ctacggcctg cggctcagtg
    12601 acagccgggc tggtcttgcc cgttaacgac gtctagtttt tcccgggatg gtgcgcgacg
    12661 gtcagtcttg ctgtggcaag cggggcaagg ttgtgcccat cggaagaagc gcacaggtct
    12721 tcatggtttg aggtgttgag gggacaagta cggcggaact gaacagacga ccgggctgtc
    12781 ttagtggggg agcgaaaact ccacgttcac atcttttccg agatag
//
LOCUS       DAORRQ010000085        12654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_241_length_12654_cov_8.112469, whole genome shotgun sequence.
ACCESSION   DAORRQ010000085 DAORRQ010000000
VERSION     DAORRQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_241_length_12654_cov_8.112469"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3530)
                     /locus_tag="PK224_18260"
     CDS             complement(<1..3530)
                     /locus_tag="PK224_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRB17746.1"
                     /translation="MTSTTPGREPNRLIQQTSPYLLQHAYNPVDWYPWGPEALAQAAK
                     LKRPILLSIGYSSCHWCHVMERESFENEAIARIMNQHFVCIKVDREERPDLDEIYMQA
                     TLALNRNQGGWPMTVFLTPDQKPFFAGTYFPPEDRWGRPGFPTLLKKIAEYWEKDHAG
                     VLTQAATLTARLQDGSHAPSPTTVGEAELDMAVTQFAEDFDAKLGGFGGAPKFPPATG
                     LSLLLHCYHRTKDSHTLTMVRTTLDAMAAGGIYDQIGGGFARYSTDERWLVPHFEKML
                     YDNALLARVYVEAFQVTGDQNYRRVACETLDYILKEMTSPEGGFYSATDADSEGVEGK
                     FFVWTPDEILAVLSNEEDVRRICAYYDVTTAGNWEHKNVLHTAKSVALVAKELGLTVE
                     DLQATIDRVKPLLYAARAKRVPPGLDDKVITAWNGMMISAMAEAGRVFGIPRYRAAAE
                     RASEFLLTTLSKTDGRLLRTYRAGTAHLDAYLEDYAYFAEGLIDTYEAGGNERYLSAA
                     VRLAERILADFSDSQQGGFFTTATGHEALIMRSREGPDGATPSGNAVAAAALARLSYH
                     FGREDFRQAAAAAVRAYGRQIARYPRAFAKSLTVVDLLTSGPVEIAVIGASGDSNTVA
                     LCAAVSRTYIPNRVIAYRTSQQSEPTHPLLQGKALVGGKAALYVCRNFACRQPITDPA
                     DLPALLDPSQKAAPSLTAPEQKVLSGNLYPGAATVQGTAGYAARKIHDATGAGSLANG
                     FGPLGATGLTVSRLGFGTYRVGQREAEHREALLKAIRSGCNLIDTSTNYMDGESEQVV
                     GSVLQQLIRAGEVAREEIIVVSKIGYVQGQNLAQAKTREKSGKPYPDMVKYGDDIWHC
                     IHPEFLADQLTLSLDRLGLATLDVCLLHNPEYFLSHATRLGAGEQRELSTLRDEFYAR
                     LQLAFEYCERQVEAGRLRGYGVSSNTSTASPDEPGATSLSRMIEAAKAAAKNVGAAAH
                     HFTVLQCPMNVHESGAALIKNTGPDDGSTLLDEALREGVAVLVNRPLNAMPAQRGGVV
                     RLADVPVPAPEADFETQRQKVALLEEEYRKDLAPAVAHSGQGMLPADFFRWADELNRI
                     RTQVQGLEHWEQIETQMIAPHVNQVLRALSEAFTGTIAEQWEAWRDRYVPELLALLRT
                     LHREASERSRLRAEDLHRTIDPLLPEERRKA"
     assembly_gap    3751..3787
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4352..5794
                     /locus_tag="PK224_18265"
     CDS             4352..5794
                     /locus_tag="PK224_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HRB17747.1"
                     /translation="MTTDDIGPYSNYRLTVRLALLNKPGIFAKVAGLLAEEGANLGAV
                     DIVSANAERMIRDITFDVQNEAHGERVLERLEALPEVNVLSASDRIFLLHLGGKIRVQ
                     SKVPVNTRNVLSMIYTPGVGRVCQAIAKDPSKAYAFTIKSNSVAVVTDGSAVLGLGNL
                     GPAAALPVMEGKVMLFKELAGIDAWPICVSTQDPDEIIRIVRGIAPGFGGINLEDISA
                     PRCFEIERALKTSLDIPVMHDDQHGTAVVLLAALTNALKVVGKRMEEVRIVVNGLGAA
                     GTACCRILLAAGASHLIGCDKEGIVLMGDAEELRACRTDLQACIRRDQPRGTLHDALK
                     GADVFIGLSVGNVLNREDLERMNQDRIVFAMANPDPEIEPRVGDEASRIFATGRSDFP
                     NQINNALSFPGIFRGALDVQASEINEAMKLAAAEAIAGCVPAEALSEDYIIPSVFDRD
                     VVPRVAKTVAAAARATGVARRRIRIDDDLR"
     gene            5942..7201
                     /locus_tag="PK224_18270"
     CDS             5942..7201
                     /locus_tag="PK224_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HRB17748.1"
                     /translation="MTPTTIEMIGTALFAVAILHTFSTAFFEHLAHTRPAHAGLWHLL
                     GEVEVVFGFWALVLIAVMFVTDGPAAATHYLDSRNFTEPLFVFAIMVIAGTRPILHAA
                     TSGVRLLSKALPLPHNLSAYFTILTLVPLLGSFITEPAAMTLAALMLRDRFFRNGLSK
                     PLRYATLGVLFVNVSIGGTLTPFAAPPVLMVAGTWHWDFPFMMGNFGWKAAVVVVVNA
                     LAAALLFRRELCRISAEGRETAHGRVPFTMVVLHLVFLGIVVLFAHHPVMFLGVFLFF
                     LGVAQAYERHQDRLILREGLLVGFFLAGLVVMGGQQQWWLQPILLSMSNDAVFFGATI
                     LTAFTDNAALTYLGSLVEGLSDGFKYALVAGAVTGGGLTIIANAPNPAGVAMLRGHFE
                     DESINPLGLFLGALPPTILAALGFWCL"
     gene            7317..7946
                     /locus_tag="PK224_18275"
     CDS             7317..7946
                     /locus_tag="PK224_18275"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRB17749.1"
                     /translation="MAFSTNRLFKYQRGMRRRIGMLRAKNVDSMEFAVDFMMQERVDS
                     YFRIEPGLEEVERSLQQIEEELELVRDVSGAIRLESRLEFVEDRWDELDSEVRERPRR
                     RRRKINLADFLKAAGGEGSPTGATNEVTNAYDAYQILGLEFGTPLADVTTSFRQRAKE
                     LHPDARNGDRSSEPELRRILEAYQFLKEYLSLSNTEPPISRGAEYRPTE"
     gene            7950..9155
                     /gene="rnd"
                     /locus_tag="PK224_18280"
     CDS             7950..9155
                     /gene="rnd"
                     /locus_tag="PK224_18280"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRB17750.1"
                     /translation="MATLEPEQTFITSDESLDALCDQLINSSVIAIDTEFMGEEHFIP
                     RLELIQVAAEGVAAVIDFPAVQETAPMARFWELVCDARIEKVLHAGRQDLELFAHHAG
                     RLPKPFFDTQIAAAMVGYGAQTAYANLVQRVQGVKLDKAHTFTNWSQRPLSREQLIYA
                     LDDVTFLLPVHQHLRQKLSVMGRSEWVDEEFARLEVSLGAQARDPQERYQRIRGWDSL
                     KPRAAGVLRDLAAWREGEARRRNVPRGRVMRDEVLVQLARQTPRTLDQLRGMRGMYSS
                     EVERNGQALLATMQQALARPESEWPEVPRDRKPDPESTGVLELLQAVLKSRASVENIA
                     PTLIATTGDLQALVERASPVEEMDIPVLRGWRRQLVGETLLSVLSGDLKVWIDPQLGR
                     LRFGHLDHA"
     gene            complement(9358..9600)
                     /locus_tag="PK224_18285"
     CDS             complement(9358..9600)
                     /locus_tag="PK224_18285"
                     /inference="COORDINATES: protein motif:HMM:NF040329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17751.1"
                     /translation="MFAFSSVDRVSLLGRILQRLNLRFPSLFVMFALLTLADIIVPDV
                     IPLADEIGLALLTLLLGLWKERRTQSRAQATRGATR"
     gene            complement(9682..10032)
                     /gene="arsC"
                     /locus_tag="PK224_18290"
     CDS             complement(9682..10032)
                     /gene="arsC"
                     /locus_tag="PK224_18290"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRB17752.1"
                     /translation="MAEITIYQKPTCSTCREAVRIVRESGQPFKAINYYEQPFTKSRL
                     KALLKKAGLTAREVLRTKEDLYKSLKLSDPALSEDHVLDLMVLHPDLIQRPLVEKGDK
                     AMLARPADTITQML"
     gene            10135..10512
                     /locus_tag="PK224_18295"
     CDS             10135..10512
                     /locus_tag="PK224_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17753.1"
                     /translation="MPIFGTDTSDQAGKFSCASAAQRTLKDLRTKRKGQPVFVVGHRL
                     ERKGKEATFEVFNDRLAIIRFDDDAKLGYDPLELLLPTEIDEQGVAYFEIRTCRQCEQ
                     FFPLTADECDADEEPTACPDCAS"
     gene            complement(10648..11037)
                     /locus_tag="PK224_18300"
     CDS             complement(10648..11037)
                     /locus_tag="PK224_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17754.1"
                     /translation="MSWFRLRAWGIVTSLTLSLFAPAWADDDTVVFAVVSDVPKDRSR
                     VSAKALLDGAVSDLKLLPGELILGNPVWRTLEVCHALKLEGMKIGDGFKVTSARVLDA
                     SMLPMALQGYAGDCLIKKAVEIAPLAD"
     gene            complement(11041..11277)
                     /locus_tag="PK224_18305"
     CDS             complement(11041..11277)
                     /locus_tag="PK224_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17755.1"
                     /translation="MSGVTRILVLLGLLVLGVALFTLGRDERPAASSVEPRIGRSTLS
                     HGGPGAGSIPVAPAEAPSADRRIPSNQSSYATTR"
     gene            11502..12635
                     /locus_tag="PK224_18310"
     CDS             11502..12635
                     /locus_tag="PK224_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRB17756.1"
                     /translation="MPHDFMPGLAGVPAAKSAISDVDGTRGVLEYRGIRVEELCQHSS
                     FLETSYLLLFGRLPTAPELAQWVDDVTHHRRIKFRIVDLLKVMPEHGHPMDALQAAVA
                     ALGMFYPGRNVKDVANNYWSAVRLIAKLPTIVAAWARLRRGDEYIPPRDDLPFSDNFL
                     YMLTETVPHPLWSEVFDDCLILHAEHTMNASTFAGMVTASTLADPYTVVASSIGALKG
                     PLHGGANEEVVMMLREIGTPAQARAAVEARLEGKHKLMGFGHRVYKVKDPRATVLQDL
                     CMRLFNVCGTSPLYEVAVAVEQLAGERLTDKGIYPNVDFYSGIIYDKMGIEVDLFTPL
                     FAMARVSGWLAHWLEQLRENKLYRPDQIYSGEHDRHYVPIDKR"
BASE COUNT         2483 a         3901 c         3718 g         2515 t
ORIGIN      
        1 gcctttcgtc tctcttcggg caacagcgga tcgatggttc gatgcaggtc ttcggcgcgc
       61 agacggctgc gttcactggc ttcccgatgc aacgttcgca ggagcgcgag gagctccggt
      121 acgtaccggt cacgccaggc ttcccactgc tcagcaatgg tacccgtaaa cgcttcggag
      181 agcgcgcgaa gcacctgatt cacgtggggt gcaatcatct gcgtctcaat ttgttcccaa
      241 tgttccaagc cctgcacttg cgtgcggatg cggttcagtt cgtcggccca tcggaaaaag
      301 tctgcgggaa gcataccttg gccgctgtgg gccaccgccg gcgcaagatc ctttcgatac
      361 tcttcttcca aaagggcgac cttctgtcgt tgcgtctcga agtccgcctc cggcgctggc
      421 accggcacat cggccaatcg caccacgccg ccgcgctggg ccggcatcgc attcagcggg
      481 cgattcacga gcaccgcgac accttccctc agggcttcat ccagcaaggt gctgccgtcg
      541 tccggacccg tattcttgat caacgctgcg ccggactcgt gcacattcat cgggcactgc
      601 aagaccgtga aatggtgagc ggccgctccg acgttctttg ccgccgcctt cgcggcttca
      661 atcatgcgag acaaagacgt ggcgccgggc tcatccggag aggccgtgga cgtattggac
      721 gaaacgccat acccgcgcag ccggcccgct tcaacctgcc tttcgcaata ctcgaatgcc
      781 agttgcaacc gcgcataaaa ttcatcgcgt agcgtggaca gctcccgttg ctcaccggcc
      841 ccgagccggg tcgcatggga caggaaatac tccgggttgt ggagcaaaca gacatccagc
      901 gtcgccaatc ccaggcgatc cagcgacagc gtcaattgat ccgccagaaa ctccggatgg
      961 atgcaatgcc agatgtcgtc gccgtacttc accatgtccg gatagggctt gcctgatttc
     1021 tcccgcgttt tggcctgcgc cagattttgg ccctgcacat agccgatttt cgacacgacg
     1081 atgatttcct cgcgtgccac ttcaccggca cggatcaatt gctggaggac cgaaccgacc
     1141 acttgttcgc tctcgccgtc catgtaattc gtcgaggtgt cgatcagatt acagcccgag
     1201 cgaatcgcct tgagtaaggc ctctcgatgt tccgcttccc gttgcccgac ccggtacgtc
     1261 ccgaatccaa gacggctcac ggtcagtcct gtcgccccga gcggtccgaa cccgttcgcc
     1321 aacgagccgg ccccggtggc atcgtggatc ttccgggccg cgtaaccggc cgtgccctga
     1381 acggtcgcgg cccccggata caaatttccg ctcaagacct tttgttcggg cgcggtcaac
     1441 gacggtgcag ctttttgcga cggatcgagc agggcaggga gatccgcggg atcggtgatc
     1501 ggctgtcggc aggcaaaatt ccggcagaca tacaacgccg ccttgccgcc gaccagagcc
     1561 ttcccttgca acagcggatg ggtcggctcg gactgctgcg atgtccgata ggcgatgaca
     1621 cgattcggaa tataggtccg gctgactgcc gcgcataacg cgacggtgtt ggaatcgccg
     1681 gatgccccga tgacggcaat ttctaccggg ccgctggtca acaaatcgac gacggtcaga
     1741 ctcttcgcaa acgcgcgcgg gtagcgggcg atctggcgcc cataggcgcg cacagccgca
     1801 gcagccgctt gccggaaatc ttcccgcccg aagtgatagg acaatcgggc cagcgccgct
     1861 gccgcgacgg cattcccgct cggcgtggct ccgtccggac cttcacgact gcgcatgatc
     1921 agggcttcat ggcccgtggc ggtggtgaag aatccgccct gttgactgtc ggagaaatcc
     1981 gccaggatgc gctccgcgag ccgcaccgcc gcagacaggt accgctcgtt gccgcctgct
     2041 tcataggtgt cgatcaagcc ttcggcaaaa taggcatagt cttccaggta ggcatccagg
     2101 tgagccgtgc cggcgcgata ggtccgcaac agtcgtccgt cagtcttgga gagagtcgtc
     2161 aggagaaact cactggctcg ctcggcagca gcccggtatc gcggaatgcc gaacacccgc
     2221 ccggcttccg ccatggcgct gatcatcatc ccgttccagg ccgtgatgac cttgtcatcg
     2281 agtccgggcg ggacccgttt cgcgcgcgcc gcatagagta acggcttgac tcgatcgatg
     2341 gtcgcctgca ggtcctcaac ggtcagcccc aactctttcg ccaccaacgc gacggacttg
     2401 gccgtatgca gcacgttctt gtgctcccag ttgccggccg ttgtgacatc gtaataggca
     2461 cagatccgcc gcacgtcctc ttcgttcgag agcactgcga gaatctcatc cggcgtccag
     2521 acgaagaatt tgccctccac cccttcagag tcggcatcgg tcgctgaata gaacccgccc
     2581 tcgggggacg tcatttcctt cagaatatag tcgagggtct cacaggccac gcggcggtag
     2641 ttttgatctc cggtcacttg aaacgcttcg acatagacgc gagccagtag ggcattgtcg
     2701 tacagcatct tctcgaaatg aggcaccagc caccgttcgt cggtcgaata gcgcgcgaaa
     2761 ccgccgccga tctggtcgta gatcccgccg gccgccatgg catccagcgt ggtacggacc
     2821 atcgtgagcg tatgcgaatc ctttgtgcgg tgataacaat ggagcaacag tgagagcccg
     2881 gtggcagggg ggaacttcgg ggcgccgccg aatcctccga gcttggcatc gaagtcctcc
     2941 gcaaactggg tcacggccat gtcgagctcg gcttcgccga ccgtggtcgg ggaaggggcg
     3001 tgacttccgt cctgcaggcg cgcggtgagg gtcgcggctt gggtcagtac gcccgcatga
     3061 tctttttccc aatactcggc aattttcttg agcagagtgg ggaagcccgg acggccccag
     3121 cggtcctccg gcgggaaata ggtgccggca aagaacggtt tctgatcggg ggtgaggaac
     3181 acggtcatgg gccagccacc ctgattccgg ttcagcgcca gcgtggcctg catgtagatc
     3241 tcatcaagat cgggccgctc ttcgcgatcg actttgatgc agacgaaatg ttgattcatg
     3301 atccgggcga tcgcctcgtt ctcaaacgat tcccgctcca tgacatgaca ccaatggcag
     3361 gaggaatagc cgatcgagag gagaatcggc cgcttcagtt tcgcggcttg cgccaacgct
     3421 tcaggtcccc agggatacca atccaccggg ttataggcgt gttgcaggag atagggacta
     3481 gtctgctgta tgaggcgatt cggctcgcgg ccgggggttg tgctggtcat gcgcgaaccg
     3541 tatcacgcac cgattgaatg ggtcaatgaa gcgaaggaga gaaggaaggg caaccgcgcg
     3601 aggcaaaaaa attggctcgc gccggcatcc ggagtcgcga gccaatttgg actacgaaag
     3661 aacgtcggaa gcggggatca gtgtccgccc ttcttttcgt ccttcttctt ctcttcgccg
     3721 aacacggtct gggacaggtg tccacccttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnngga gaagtggccg cccttcttct cttccttctt cttgtcgtca ccggcgaacg
     3841 acggggcggt gaatgcaacg agaatggctg ccgctacgaa tgcaaataga gcacgcttca
     3901 tggaaacccc tccttgaaag gttgagaata taagtgccca ccacaagggc gggccgaggg
     3961 tagccaacct gtcaaaaaaa gtcaacgaaa ctcttcacca tcttgttttg cctccgcaga
     4021 cagcccgcca gggcctggga cgccccctgg cgaacagctc cacaatcttt tccggttgga
     4081 ttcgaatcaa agctgaattc tcaatatgat atgagctgaa catcaaccaa tcattgcgcc
     4141 ttcatcctct tcagacttgc ccatggttct cgtgtcatcg ttcctgcgaa tctcacatgc
     4201 cgactgcgtg catcagaagg tctttggcga cagttcctat tcggtgggac gcggcagccc
     4261 tgcgcagcga acaagccctt tagtccgccc ccgcgtatca tccactgtga tacactcgtg
     4321 aacaaggcca ctcacctccg ggtcgcagct tatgaccacc gatgacatcg gtccctattc
     4381 caactaccgc ctgaccgtcc gcctcgcact cctcaacaaa ccaggtatct tcgcaaaagt
     4441 ggccgggttg ctggctgaag agggggccaa cctcggggcc gtagacatcg tgtcggcgaa
     4501 tgccgaacgc atgattaggg acattacctt cgacgtgcag aatgaggccc acggagaacg
     4561 tgtcctcgaa cggctcgaag ccctgccgga agtgaatgtg ctttcggcat ccgaccggat
     4621 tttcctcctc catctgggcg gaaaaatcag agtccagagc aaagtcccgg tcaatacgcg
     4681 caatgtcctc tcgatgattt acacacccgg ggtggggcgt gtctgccagg ccatcgcaaa
     4741 agacccatcg aaagcctatg ctttcacgat caagagcaat agcgtggccg ttgttaccga
     4801 cgggtctgcg gtactgggtt tgggtaacct cggccctgcc gcagcgctgc cggtcatgga
     4861 aggcaaggtc atgctgttta aggagttggc cggcatcgat gcctggccga tttgtgtttc
     4921 cacacaggac cccgatgaaa tcattcgcat cgttcgcggc atcgcgcccg gtttcggcgg
     4981 catcaacctg gaagacatca gcgcgccacg ttgcttcgag attgaacggg cgctcaagac
     5041 gtccctcgac attcccgtca tgcacgacga tcagcacggt acagccgtcg tcctcctggc
     5101 cgctctgacc aatgcgttga aggtggtcgg aaaacggatg gaggaggtcc ggatcgtcgt
     5161 gaacggtctg ggcgcggccg gaaccgcctg ctgccgtatt ttgctcgccg ccggggcgtc
     5221 ccatctcatc gggtgcgaca aagaaggcat cgtgctgatg ggtgatgcgg aggaactgcg
     5281 agcctgccgc accgatcttc aagcctgcat ccgtcgggat cagccgcgcg ggacactcca
     5341 cgatgcctta aagggcgccg acgtgttcat cgggctctca gtgggcaatg tgctgaaccg
     5401 ggaggatctc gaacggatga atcaggaccg gatcgtcttc gcgatggcca accctgatcc
     5461 ggaaatcgag ccgagggtgg gcgatgaggc ctcccggatc ttcgccaccg gccgatcgga
     5521 ctttcccaac cagatcaaca acgccctgtc gtttcccgga attttccgcg gggcgctcga
     5581 cgtccaagcc agcgagatca acgaagccat gaagctggcg gcggccgagg ccattgccgg
     5641 ttgtgtgccg gccgaggccc tgtcggagga ctacatcatc cccagcgtgt tcgatcgcga
     5701 cgtggtgccg cgcgtcgcga agacggtggc cgccgccgcg cgcgcaaccg gtgtggcccg
     5761 ccgccgtatc cggatcgacg acgacctccg ctgaccgtgc aaccctcatc tccctaccgt
     5821 ttgttttctt gcttggcgta gtcctcgcac cccatgtaca gtacgcacat attctgatgg
     5881 accggttcgt ccgcgcgtca tccctagtcg gggttcgccg cgaccgccct gagaaatcaa
     5941 cgtgacgcca accactatcg aaatgatcgg gaccgccctg tttgccgtcg cgatcctgca
     6001 tactttctcc accgcatttt ttgagcacct ggcccataca agaccggcgc atgccgggct
     6061 gtggcatttg ctcggggaag tcgaagtcgt cttcggcttc tgggcgctcg tcctcatcgc
     6121 cgtcatgttc gtcaccgatg ggccggctgc tgcaacacac tacctcgact cacgcaactt
     6181 cacggaaccg ctgttcgtct tcgccatcat ggtcatcgca ggcacgcgcc cgattctgca
     6241 tgccgccacg agcggagtgc ggctgctcag caaggccctc ccgttgccgc acaatctgag
     6301 cgcctatttc accattctga cgctggtgcc gttgctgggg tcattcatta ccgaacccgc
     6361 agccatgacc ctggccgccc tgatgctgcg cgaccggttt tttcggaacg gcctttcgaa
     6421 acccttgagg tatgccaccc tgggagtgct gtttgtgaat gtttcgatcg gcggcacgct
     6481 cacaccgttt gccgcgcctc cggtcttaat ggtggccggc acctggcact gggacttccc
     6541 gttcatgatg ggaaacttcg gatggaaagc cgcggtcgtg gttgtcgtca atgcgctggc
     6601 tgcggcgctg ctgttccgta gggagctctg tcgaatctct gcagagggcc gcgaaacagc
     6661 acatggcagg gtgcccttca cgatggtcgt gctgcacctg gtctttcttg gaatagtggt
     6721 gctcttcgcc catcacccgg tgatgttcct gggagtcttt ctgttcttcc tgggtgtcgc
     6781 ccaggcttac gaacgacacc aggatcgctt gatcctgcgc gagggcttac ttgtgggatt
     6841 ctttctggca ggcctggtcg tgatgggcgg gcagcaacag tggtggctgc aaccgatcct
     6901 gctgagcatg agcaacgatg cggtcttctt cggggcgacc atcctgacgg ccttcaccga
     6961 caatgcggcg ctgacctatc tcgggtcgct cgtggaaggc ttgtcggacg gatttaaata
     7021 cgcgctggtg gccggtgcag tcacgggcgg cggcctgacc atcatcgcca atgcgccgaa
     7081 ccctgccggc gtggccatgc ttcgcggcca cttcgaggat gagtccatca atccgctggg
     7141 actcttcctg ggtgcgttgc cacccacgat cctcgctgcg ctcggctttt ggtgcctgta
     7201 gacctctcaa cccttcacca gaccccgatg ctccgctctt atcgaagcgc aaacggttgt
     7261 gctaagatgc ccacgacctt gccggaaccc tggagggtca cgcccaacga ggacttgtgg
     7321 ccttttcgac caatcgcctg tttaaatatc agcgaggcat gcggcggcgc atcggcatgt
     7381 tgcgcgccaa gaatgtcgac agcatggagt ttgccgtcga cttcatgatg caggaacggg
     7441 tcgacagcta cttccgcatc gaacctgggc tcgaagaagt cgaacggtcg ctgcaacaga
     7501 tcgaagagga attggaactg gtgagggatg tttcgggcgc cattcggctg gagtcccgat
     7561 tggagttcgt cgaagaccgc tgggacgaac tcgacagtga agtgcgggaa cgtccgcgcc
     7621 gccggcgccg caaaatcaac ctggccgact ttctcaaagc cgcgggaggg gagggaagtc
     7681 ccaccggcgc gaccaatgaa gtcaccaatg cctacgatgc ctatcagatt ctgggcctcg
     7741 agttcggcac tccgctcgcc gatgtgacca cgtccttccg tcaacgcgcc aaagaactcc
     7801 accccgatgc gcgcaacggc gaccgcagtt cagaaccaga gctccggcgc atcctcgaag
     7861 cctatcaatt tctcaaagaa tacttgagcc tgagcaacac cgaaccgccg atttcacgcg
     7921 gggcggaata tcgtcccact gagtaaccca tggccacact ggaaccggaa cagacgttca
     7981 ttacctccga cgagtcgctc gacgcgcttt gtgatcagct gatcaacagc tccgtcattg
     8041 ctatcgacac cgaatttatg ggggaagagc atttcattcc ccgcctggag ttgatccagg
     8101 tcgcagcgga aggggtggcg gcggtcatcg acttcccggc cgtgcaagag accgcgccaa
     8161 tggcccgatt ctgggagctt gtctgcgatg cccgcattga aaaagtgctg catgcgggac
     8221 ggcaggacct ggaactgttt gcgcaccatg ccggacgtct gccgaagccg tttttcgata
     8281 cgcaaatcgc cgccgccatg gtcggctacg gcgcgcagac cgcctatgcc aacctggtcc
     8341 agcgcgtcca gggtgtgaaa ctcgacaagg cccatacgtt tacgaattgg agccagcggc
     8401 cgctgagccg ggagcagctg atctatgccc tggatgacgt gacctttctg ctcccggtgc
     8461 atcagcatct ccggcagaaa ttatccgtga tgggtcgctc ggagtgggtg gacgaggaat
     8521 ttgcccgctt ggaggtctct ctcggcgcgc aggcgcgaga cccgcaggag cgatatcaac
     8581 gaatccgtgg atgggatagc ctcaaaccac gcgcagccgg cgtcctgcgc gaccttgcag
     8641 cctggcgtga aggggaggcc cggcgtcgaa atgtgccgag aggacgagtg atgcgtgacg
     8701 aggtgctggt gcaactggcc cgtcaaacac cgcgcacact ggatcaattg cgcggcatgc
     8761 gggggatgta ttcgtcggaa gtcgaacgca acggacaggc gcttctcgcc accatgcagc
     8821 aggcgttggc gcgaccggaa tccgaatggc ccgaggtccc gcgcgatcgc aaaccagacc
     8881 ccgagtctac cggcgtgctg gagctgctgc aggctgtgct gaagtctcgt gcctccgtgg
     8941 agaacattgc cccgacgttg atcgctacca ccggtgactt gcaggccctg gtcgaacgcg
     9001 cctcgccggt ggaggagatg gacattcccg tattgcgggg atggcgccgg caactggtcg
     9061 gagaaacact cctcagcgtc ttatccggcg atctgaaggt gtggatcgac ccgcaactcg
     9121 gcagactccg cttcggccat ctcgatcacg cgtaacctcc gccgtctagc acgcattctt
     9181 catgagcact tctgctgcaa tcgcggattc gctgctacta cgcgccttcg gctggcaggc
     9241 tcgccgctcg gtccctcgac gtactgttca agtacgcctc ggtccctcac agctccgcat
     9301 gcccgtctcg caacgcgact gcgcgatttc ccgacgaact gtcatgagga atgcgagcta
     9361 gcgcgtcgcg cctcgcgtgg cttgagcgcg gctctgagtc cgacgctctt tccacaaccc
     9421 cagcaacaac gtcagcagcg ccaggccgat ttcgtcggcc agcgggatca cgtccggcac
     9481 aatgatgtcc gccagggtga ggagggcgaa catcacgaag agagacggaa agcgaaggtt
     9541 gagccgctgc agaatcctcc cgagcagcga cacgcgatca acggaactga atgcgaacat
     9601 aggtccggtc ctccgtcatg cccgccgacc tctctgcaat aacgtctgca gaagcgcccg
     9661 gctcccactt tccttggaag ctcacagcat ttgagtgatc gtgtccgccg ggcgcgccag
     9721 catggccttg tcgccctttt cgacgagcgg acgctggatc aaatccggat gcagaaccat
     9781 caggtcgagc acatggtcct cgctcagcgc cggatcggac agcttcagcg acttgtagag
     9841 atcttccttc gtccgtaaga cctcacgcgc cgtcagtccg gctttcttca gcagggcctt
     9901 gagccggctc ttcgtgaagg gttgctcata ataattgatc gccttgaacg gttgcccgct
     9961 ttcgcgcaca atccgaacgg cctcgcgaca cgttgaacaa gtgggcttct gataaatcgt
    10021 gatctccgcc ataccttcag cctctctcct tcttatgagg ttcccttgac gtaaattttt
    10081 gacctgtgtt acatggacac acgagacttc accccagagc gatactgttg acctatgcca
    10141 attttcggaa ccgacaccag cgaccaggcc ggcaaatttt cctgcgcgtc agcggcgcaa
    10201 cgcaccctga aagatctgcg caccaaacgc aaggggcaac ccgtgtttgt cgtcggacac
    10261 cgtttggaac ggaaaggcaa ggaagccacc tttgaagtct ttaatgaccg cctggccatc
    10321 attcgcttcg atgatgacgc gaaactaggt tacgacccgc ttgaactgct gctgccgacc
    10381 gaaatcgacg agcagggggt ggcgtatttc gaaatccgca cctgccggca gtgcgagcaa
    10441 ttctttccgc tcacggccga cgaatgcgac gccgacgaag aaccgaccgc ctgtcccgac
    10501 tgcgcatcgt aactgccaac tcccaggatg cgcaaaaagt ccgtccagca aggccgcagc
    10561 gagtgaagcc tcgaatcgta cccgtgcggt acggtgaggc gctgaacgat gcgagaacgc
    10621 cgctggcgga ttttttccgc atcctaacta gtctgccaac ggcgcaattt ccaccgcctt
    10681 cttaatcaag caatccccgg catatccctg cagcgccatc ggcagcatac tggcatcgag
    10741 cacccgtgcc gaggtgactt tgaatccgtc tcctatcttc atcccttcaa gcttaagggc
    10801 atgacagact tccagcgtgc gccagacggg attcccgagg atcaattctc ccggcaggag
    10861 tttcaagtcg gagacggcgc cgtcaagcag cgccttggcc gacacgcgcg atctgtcttt
    10921 cggcacatcg ctgacgacgg caaacacgac agtgtcgtca tcggcccagg ccggggcgaa
    10981 cagactcagt gtcagcgatg tcacgattcc ccacgcccgc aagcggaacc agctcatgcc
    11041 tcacctcgtg gttgcgtacg aagactgatt agaagggatc cggcgatccg cggacggggc
    11101 ttctgcgggc gcgaccggga tggatccggc gccgggcccg ccatgactca aggtactgcg
    11161 cccgatgcgt ggttcaacgg aagaggctgc cggtcgctca tcgcgcccga gcgtgaacaa
    11221 ggccacaccc aacaccagaa gcccgagcag cacaaggatc cgtgtcacac cgctcactca
    11281 atgcctcata gagagtcaca cgtcgttcga gtcagtatgc caagcgggcc gcaccaattc
    11341 aaggtgagag ctgagtcggc accgtggatc atggtaagct cagccaggca cgtgtgtgga
    11401 cagtggcgtc gaagcagcgg cacgcgggag ggcatccccg ggcgccggat gcatacgtgg
    11461 tgggtcatca ttcgtgttgt tgaagagagg agagggaaac tatgccgcac gacttcatgc
    11521 cggggcttgc cggtgttcct gctgcaaaat cagcgatcag cgatgtggat ggaacgcgag
    11581 gagtacttga atatcgcggc atccgcgttg aggaactctg ccagcactcg tcgtttctcg
    11641 aaacgagtta tctgctgttg ttcggtcgac tgccgaccgc accggagctc gcgcagtggg
    11701 tggatgacgt cacgcaccac cgacgtatca aatttcgcat cgtcgatctg ttgaaggtga
    11761 tgcccgagca tgggcatccc atggatgcgt tgcaggccgc ggtcgcggca ctcggcatgt
    11821 tctacccggg ccgcaatgtg aaggatgtgg ccaacaacta ttggtcggca gtgcggctga
    11881 tcgccaaact ccccaccatc gtggcggcct gggcgcgcct gagacgcggg gacgagtaca
    11941 tcccgccgcg agacgatttg ccgttttccg ataactttct ttatatgctg accgaaaccg
    12001 ttccgcatcc gctgtggagc gaagtcttcg acgactgtct gattctgcac gccgaacaca
    12061 ccatgaacgc ctcgaccttt gccggcatgg tgacggcttc caccctggcc gacccgtata
    12121 ccgtagtggc ctcctcgatc ggcgcgctga agggcccgct gcacggcggc gcgaatgaag
    12181 aggtcgtgat gatgctgaga gaaatcggca ccccggctca agcccgcgcg gcagtcgaag
    12241 ccaggctgga ggggaaacac aaactcatgg ggttcgggca ccgggtctac aaggtaaagg
    12301 atccgcgcgc gaccgtgctg caggacctct gcatgcgtct cttcaacgtc tgcggaacgt
    12361 ccccgctgta tgaagtggcg gtggcggtgg agcaactcgc gggtgaacga ttaacggata
    12421 aggggatata cccgaatgtc gacttttact ccggtatcat ctacgacaag atgggcatcg
    12481 aggtggatct ctttacgccg ctgttcgcca tggcgcgagt aagcgggtgg ctggcgcatt
    12541 ggctggagca actgcgggag aacaaactct atcggccgga ccagatctac tccggcgaac
    12601 atgaccggca ctatgtgccg atcgacaagc ggtagtggag tgacggttac tccg
//
LOCUS       DAORRQ010000086        12627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_242_length_12627_cov_11.121699, whole genome shotgun sequence.
ACCESSION   DAORRQ010000086 DAORRQ010000000
VERSION     DAORRQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_242_length_12627_cov_11.121699"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PK224_18315"
     CDS             complement(<1..912)
                     /locus_tag="PK224_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17757.1"
                     /translation="MPITGIKWKTNREYDIHLLRGRTLPALLNAVDFQLPDGTTQDAA
                     AYLAANADVTINFQPSFRNVLDLTVAPPTCSGFGITINNDNGEIRVPAPPGPATTIHN
                     FLLHATAEDSSDDKEYRISVRIHLHNRVTSTWLTPPILTLRPDGPTLPQTTFRRFTVR
                     AQFDDNTVGDLTNHPGLAWGPLANVEPSGRLIISVGNGPSDPAVEITATLPADLRDPA
                     NPAPPEIRASGHIRFASDWAVEPTIRTETVQIQDTWPGTINPELVPNFLFLCDGYTTD
                     DKPQFESQIRSLLGLMKKSRLTRPFDLL"
     gene            complement(1195..4515)
                     /locus_tag="PK224_18320"
     CDS             complement(1195..4515)
                     /locus_tag="PK224_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17758.1"
                     /translation="MADHLVQNATAGIGRLLSHLDIVQGDVEEARAFLKLLGWELPPG
                     LDDIGLATIDLGNFLTTLDAVIGASDADWNDDLAMAGRIANLALAIEALTRQIHDLAQ
                     TLPAKLSSFGDYVDRTQIHKELPRRLFDFLVANYVAQASPLSYAVLHLLNIIDYPYYA
                     ADPTKFQVEHVRATVNYHLFKVAVTNPDQLFTEAYGWHTPDFQSMTFLTRLSQLLQTL
                     GLRSRIQPMSPQAQEAWLGRAETSSNPPPQLITFLHEQRGSAFGVRLGLSLFGAAPTS
                     AGASDAGLGLAPIIQGHAEGSVPFPRLEDTRIEWSGNVEVLKRLAVILRPEQDLTLRK
                     GAGLSDAVNGRLTLGLRHGQSAGELKPLVQFPGGSALRYQQFAVAGGLDASSASHTET
                     FVELALSGLRFDLSLGDADGFIQGTVARDRVEAPFDLALRWSNRQGISFSGSGGLHVF
                     LPLHTTIGPLRLDAAHIGIDVGEEGIDTETSLSGRLTLGPVTATVERLGMTVNISFRE
                     GNLGLFGLSPRFKPPTGLGLAIAAPGVVGGGYLGFDPQRAEYSSMLQLELADRIALKA
                     IGLLTTRLPDGSKGYSLLILITVEGFTPIPLGLGFTLTGIGGLLGLHRTVSTSTLREG
                     LKTGTLNAILFPVDPLRNAPQLLSDLRRVFPPAAGRHVFGPMVQLRWGTPTLLTLELA
                     LLLELPSPVRLIVLGRLQVLLPNQAHPLVQIRMDALGVLDVSAGTVSLDATLYDSRIL
                     QFTLTGDMALRAGWGSQPQFILAIGGFHPRFAAPPGLPALKRLALSLADGDTLQLRCA
                     AYLAVTSNTVQFGARVDLHAAGGGFSFDGMLGFDALIQLAPLAFQVDIGAALALRYRG
                     RLLMGISFRGSLAGPTPWEVQGKATIKILFFKVSVSFERQFGTKTPPPLPAAVDVVAQ
                     VTTALADRRNWSGTLPRHEPPVVTFRDAGPTTTGPLRVHPLAELTVRERIAPLNRPIT
                     KLGTAPLVGGPTTLTVTTTGSGPTALPWKTTPVNEPFALAQFEDLREDEQLARPSFEP
                     LEAGLTFALDEVAVDDEAGLSAPIAYETLLIDPTRPPERPKPGYVLSAAVLARLAPFG
                     AAGQAAIRKRGRATTLVA"
     gene            complement(4500..6758)
                     /locus_tag="PK224_18325"
     CDS             complement(4500..6758)
                     /locus_tag="PK224_18325"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HRB17759.1"
                     /translation="MANISIHGRIIDERGAGIAGLTVRALDFDPFFSEDDVLGVGKTE
                     GDGTFLISYSPDAYRTWKVDRNPDLVAQIFGPIHADPKLFGTRLLHETPEAKDVTDSN
                     HEVGTITIHRNNIEGWLVTHTTLNPAVGNPVALFHHNRITHLIDGAKMFPAVTNAAEG
                     ATESINLMTLFFDVDNGFLSKFKSSFTHLNPPSTGCKQAAETSLEEVLKNKGGKPVNV
                     LVTNLPLSAEDTVTEVAEFFAQTAGVSTNAFNKGFALLHAKAIVVDGDHAILMGSPLK
                     QYYFSDTNHAIRDARHKGSLMHDVNTDLKGPAVSHIEKTFASIWNATGKPMLIPPPKT
                     FPDLPVTPDGDVASVQVLRTLPGGSIKRVNPSDEDLPYGETGILEAYERAIANAQRYI
                     YIENQYFTSPEIVDALIARMKDTTKPRLQIILVLNLRPDLPGYPDRQIDNVNQLRSAA
                     KTGGHHLGAYTLWSRSEKAGSGGAGNPRRYDVMPVYVHSKVAIIDDVWATAGSANLDG
                     TSLNYHEIGLIITGSILDRAIEKAKLTNDPGKFLWELFWYLFFFVFKQILFDLKTLLV
                     LLFVAYKLIFDFKETLETIRETLGDVADIPQLIADVFTRTARHALPGRSRQPSRSVEL
                     NLVMYSGIAGLPENGVVKALRHELWQEHLGLASLPDQLKTLPADPASMTWVAEWEAAA
                     THHVDAIKNDLAPPADHAPHLLPWKPETDPKEYLAALKIRTSTLRNKAQKFDFKTCKV
                     DDKKDLLPWPII"
     gene            complement(6948..7427)
                     /locus_tag="PK224_18330"
     CDS             complement(6948..7427)
                     /locus_tag="PK224_18330"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HRB17760.1"
                     /translation="MITTRFALIPGLLLTLGGCMTNATVELTKAPFDATTQLTNGTSG
                     ATKEFLDPTTEFTSSTTPGSLADGRLLRAKQKATVFAMHTHENLRADIARGQGEYLAS
                     LATLTGVSADRYPDFQATMIGSYPTLFDQQLSLSQSNERVIDLAWANGYGMPQPAVK"
     gene            8323..9057
                     /locus_tag="PK224_18335"
     CDS             8323..9057
                     /locus_tag="PK224_18335"
                     /inference="COORDINATES: protein motif:HMM:NF020452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HRB17761.1"
                     /translation="MELRHAKDDAVLSAWAKHFRNQYCRDDQIDDLCKGTRLSRAEYL
                     RTIKFPDQSDAPGPSIRAGDFGEVLVADYLEFALAYWLPRTRYDNKTVRNESTKGTDI
                     IGFKIVAPGQDDPKDALALFEAKAQLSGDTPSGRLKAAVDGSAKDEIRKAESLNAIKQ
                     RFLDRGRSADVQTVERFQNPEDRPYLEEYGAVALLSSSVFDKKDVGATTIASHPHASK
                     LTLIVIHGPDLMPLVHELYRRAADEA"
     gene            9047..12178
                     /locus_tag="PK224_18340"
     CDS             9047..12178
                     /locus_tag="PK224_18340"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB17762.1"
                     /translation="MRPEQSSRVLLAITRSKAKMFEYGLPENEHIKISRDPSRLLRLA
                     VGMLGDVAAAVSRNGTDAARVADLSSSLQFSARYFDAYAASRLAEAGDAHLLILASAT
                     YYLCDLPGSSNVLVSRIPDVPNLDAGGLEHLLWWLLRGDFSSAIEQTESPRYRDEIRR
                     VSRGLKAFYDLDTQDERQPHAATVSLRETAYRDGTPRELLFADTCCAIVSRRIENSAR
                     RSLPQYSGLPMEIWRPALAKKFFVRELWPAQRLLGQRSIFAGGSAVVQMPTSAGKSRA
                     TELIIRSAFLSGRASLAVVVGPFRALCHEIRQSLARAFRGEDVNVNELSDVFQRDFDF
                     DGLVAGHAILVMTPEKLAYVLRHLPELAGRVGVLVLDEGHQFDTGPRGVTYELLVTSL
                     KGMIPAGAQKVLISAVITNASDIAKWLTGDEGAVVAGADLLPTERSIAFASWKTLLGQ
                     LHFVAPENPDEDEFFVPRAIRSTSLALRPRETKERRFPEKGDGQSVALYLGLTLVRNG
                     AIAIFCGRKDTATGLCEALVDVAARGLAMPMPVEVSDGPEVERLASLYERNLGTGAAA
                     ARAAKIGAFTHHGNTPHGVRLAVEYAIKEGFGKFVLCTSTLAQGVNLPIRYLIVTTAQ
                     QGGEKIKVRDFHNLMGRAGRSGMHTEGSVLFANPEVYDTRGAAESKWPEFKALLQVRN
                     SEPCASTLLSALGPLKSDGGKVTLTIDPLDVVRDYVADQDGGGAWVDQAATQLTRKWF
                     STETLRRQLTERRDILAAVESFLLAHWEEGGDGESFGAGPVGEVAKRTLAYHLASAEQ
                     RAQLLQLFELLAANVTARGGDAERRRVYGRTLFGVGDTVALEKWVADNLERIAGCADL
                     DELFDIVWPCVAARIKSDTFQKWQPVETREVFAKRWIAGDSFGSLHDSMTAASVRIGL
                     GAKPRKIKVENIVEMGENALGFDGAHLLGAIAELFELLAPEDESEGEPLRLLQVLQKR
                     LKYGLPAGAPILLYEAGFADRPLALELAAAFPDISSRIEMRDAMREGQPAVEAVLTRY
                     PRYFTELLERVSG"
BASE COUNT         2341 a         3649 c         4189 g         2448 t
ORIGIN      
        1 gaggaggtcg aagggccgcg tcagccggct tttcttcata agccccaaca ggctcctgat
       61 ttgggattca aattgcggtt tgtcgtcggt ggtgtagccg tcgcacaaga agagaaaatt
      121 ggggacgagt tccgggttga tcgtccccgg ccaggtgtcc tggatttgca cggtctcggt
      181 tcgaatggtg ggttccaccg cccagtcaga tgcgaagcgg atgtgaccgc tcgccctgat
      241 ctccggtggc gcagggttcg caggatcccg caggtcggcc ggaagcgtgg ccgtgatctc
      301 cacggccggg tcgctcgggc cattcccgac cgaaatgatc aatcgacctg atggttccac
      361 gttggcgagg ggaccccacg ccagtccggg gtgattggtg aggtcaccga cggtattgtc
      421 atcgaactgt gcgcggaccg tgaaacgcct gaacgtggtt tgcggcaagg tcggcccgtc
      481 cggtcgcaag gtcaggatcg gaggtgtcag ccaggttgag gtcacgcggt tatgaagatg
      541 aatccggacg ctgatgcggt attccttgtc gtcgctcgaa tcttctgccg tggcgtgcag
      601 gagaaaatta tgaatcgtcg tggccgggcc gggaggcgcg ggcacacgga tttccccgtt
      661 gtcattgttg atcgtgattc cgaagccgga gcaggtcggc ggcgcgacgg tgaggtcgag
      721 cacgtttcta aacgacggtt ggaagttgat ggtgacgtcg gcattggcgg ccaggtaggc
      781 cgcggcatct tgagtggtac cgtcagggag ctggaaatcg acggcattga ggagggccgg
      841 taaggttctc ccacgcaaca ggtggatgtc gtactcccga ttggttttcc atttgatccc
      901 ggtgatcggc atgacggctt gcgttgttcg ttgtggcgtt cgacgttaac aaaagtcagg
      961 cggcaatccc gtccgcccga gcgtctcgaa caggcgacga aagtctacga ccggacgaac
     1021 cgaaaggtac tccgctcgtg aaggactgtc aaacccatct ctgcggtaaa tacgtgcatt
     1081 atggtggttc agcgcaatca atgttcgcac gtttcgccgc ccgctctagg cccgtacggt
     1141 ggtacgaagg tacgtgtggt ggaagaggcc ggattgagag ggtacgtagt catctcaggc
     1201 tacaagcgtc gtggcgcgcc cgcgtttgcg aatcgcggct tgcccggcgg cgccgaaggg
     1261 cgcgagccgg gccagcaccg ccgccgacag cacatagccg ggtttcggcc gctccggcgg
     1321 tcgtgtcgga tcgatcagca aggtctcata ggcaatcggc gctgagagtc ccgcctcatc
     1381 gtccactgcc acctcgtcga gcgcaaaggt cagccccgcc tccagcggtt cgaacgaggg
     1441 ccgcgccaac tgctcgtcct cccgcaggtc ttcgaattgc gccagggcaa agggctcgtt
     1501 taccggcgtg gtcttccacg ggagcgccgt cggcccgctg cccgtcgtgg tgaccgtcag
     1561 cgtcgtcggc ccgcccacca gcggcgccgt ccccagcttc gtgatcggcc ggttgagcgg
     1621 cgcgatccgc tcccgcaccg tcaactcggc cagcggatgc acccggagcg gaccggtggt
     1681 cgtcggcccc gcatcgcgga acgtcaccac cggcggctca tggcgcggca aggtcccgct
     1741 ccaattgcgc cggtcggcca aggcagtcgt gacctgcgcc accacatcca ccgccgccgg
     1801 gagcgggggc ggggtcttcg tgccgaactg ccgctcgaag gacaccgaga ccttgaagaa
     1861 caggatcttg atcgtggcct tgccctgtac ctcccagggc gtggggccgg ccaggctgcc
     1921 gcgaaaggag atgcccatca gcaaccggcc gcgatagcgc agggccaggg ccgcgccgat
     1981 gtccacctga aacgccaggg gtgccaactg aatcaaggca tcgaagccga gcatgccgtc
     2041 gaaactgaac ccgccgccgg cggcgtgcag atcgacccgg gcgccgaact gcacggtgtt
     2101 ggaggtgacg gccagatagg cggcacagcg cagctgcaac gtgtcgccgt cggcgagact
     2161 caaggccaat cgtttaagcg cgggcaagcc cggcggggcg gcaaagcgcg ggtggaagcc
     2221 gccgatggcc aggataaact gcggctggct cccccagccg gcccggagcg ccatgtcgcc
     2281 ggtcagggtg aactgcagaa tacgcgaatc gtagagcgtg gcatccaggg agacggtgcc
     2341 ggccgaaaca tccaggactc ccagggcgtc catgcgaatc tgcacgagcg gatgcgcctg
     2401 gttgggcaac aggacttgca accggcctaa gacgatcaac cggacgggcg acggcagttc
     2461 gagcagcagg gcgagctcga gcgtcaggag cgtgggcgtg ccccagcgca gttgcaccat
     2521 ggggccgaag acatggcggc cggcggccgg tggaaacacg cggcgtagat cgctcagcaa
     2581 ttgcggggcg ttccgcaacg gatccaccgg aaacagaatc gcattcagcg tgccggtctt
     2641 gaggccctcg cggagcgtgg acgtggagac ggtgcgatgc aggccgagta ggccgccgat
     2701 gccggtgagg gtgaagccca ggcccaaggg aatcggggtg aagccctcga cggtgatcag
     2761 gatcaagagg gagtagccct tactgccgtc gggcaggcgc gtagtgagca atccaatggc
     2821 tttgagcgcg atgcggtcgg ccagttccaa ctggagcatg ctgctgtatt cggcccgttg
     2881 gggatcgaag cccaaatacc cgccgcccac gacgcctggg gcggcaatcg ccagtcccag
     2941 cccggtcggg ggtttgaagc gcggtgagag accgaagaga ccaaggtttc cttcgcggaa
     3001 ggagatgttg acggtcatgc cgagtcgctc aacggtggca gtgaccggcc cgagtgtcag
     3061 tcgtccgctc aggctggttt cggtgtcgat gccctcttcg cccacatcga tgccgatgtg
     3121 ggccgcatcc agcctcagcg ggccaatggt tgtgtgcagc gggaggaata cgtgaagccc
     3181 gccgctgccg gagaacgata tcccttgtcg attgctccaa cgaagcgcga ggtcgaacgg
     3241 ggcctcgaca cggtcgcggg ccacggtgcc ctggatgaag ccatcggcgt ctcccagcga
     3301 caggtcgaac cggagccccg agagcgcgag ttccacgaat gtctcggtgt gactcgccga
     3361 ggaggcgtca aggccgcctg cgacggcgaa ctgctgatag cgcagggccg agccaccggg
     3421 gaactgcacc aacggcttga gctcgccggc cgattggccg tgtcgcagcc ccaacgtcag
     3481 ccgaccgttg acggcgtcac tgagtccggc tcccttgcga agggtgagat cctgctctgg
     3541 cctgagaatc accgccaacc gcttgagcac ttccacgttg ccggaccatt cgatgcgcgt
     3601 gtcctccagg cgagggaacg gaacggaacc ctctgcgtgg ccttgaatga tgggcgcgag
     3661 gccgagaccg gcatcactcg cgcctgccga cgtgggggcg gcgccgaaga gcgagaggcc
     3721 cagtcgcacc ccgaatgcgg agccacgttg ctcgtgcagg aaggtgatga gttgcggcgg
     3781 tgggttggaa cttgtttcgg ctcgtcccag ccaggcctct tgcgcctggg gactcatggg
     3841 ttggatacga ctccgtagcc ccagcgtttg cagcagctga ctcagcctgg tgaggaaggt
     3901 catcgattgg aagtcgggcg tgtgccagcc gtaggcctcg gtaaagagct ggtccgggtt
     3961 cgtgaccgcc accttgaaga ggtgatagtt caccgtggcg cgtacatgtt ccacctgaaa
     4021 tttcgtcggg tcggcggcgt agtaaggata gtcgatgatg ttcagcaggt ggaggacggc
     4081 gtaactgagc ggcgaggcct gggccacgta attcgccacg aggaagtcaa acaaccgtct
     4141 cggcagttcc ttgtgaatct gcgtgcggtc cacatagtcg ccgaaggatg acagtttggc
     4201 gggcagagtc tgcgccaggt cgtgaatctg ccgcgtgagg gcttcgatcg cgagcgcgag
     4261 gttggcgatg cggcctgcca tggccaggtc gtcgttccag tcggcatcgg acgcgccgat
     4321 aaccgcgtcg agtgtggtga gaaagttgcc gaggtcgatc gtggccaagc cgatgtcgtc
     4381 gaggccgggc ggcagttccc agcccaacag tttcagaaac gcgcgcgcct cctcgacatc
     4441 gccctggacg atgtcgaggt gcgacagcag ccgcccgatc ccggcggtgg cgttctggac
     4501 tagatgatcg gccacgggag cagatccttc ttgtcgtcga ccttacaggt tttgaaatcg
     4561 aacttctgcg ccttgttgcg cagggtggac gtgcggatct tgagcgcggc cagatattcc
     4621 ttcgggtcgg tctccggctt ccagggcaac aggtgcggcg catggtcggc ggggggcgcc
     4681 aggtcgttct tgatggcatc cacatggtgt gtggcagcgg cttcccactc ggcgacccac
     4741 gtcatggaag caggatcggc cggcagggtt ttgagctgat ccggcagcga ggctagcccc
     4801 aggtgttcct gccacagctc gtggcgcaag gccttgacca cgccgttctc cggcaggccg
     4861 gcaatgccgc tgtacatgac caggttgagt tccacgctgc gcgacggctg acgtgaacgg
     4921 ccggggaggg cgtgtcgggc ggtgcgtgtg aagacgtcgg caatgagttg aggaatgtcg
     4981 gccacgtcgc ccaacgtttc gcggatggtc tccagcgttt ccttgaagtc gaagatcagt
     5041 ttgtaggcga cgaagagcag gacgagcaac gtcttgagat cgaacaggat ctgcttgaag
     5101 acgaagaaaa agagatacca gaatagttcc cagagaaatt tgcccggatc gttggtgagt
     5161 ttcgccttct cgatcgcgcg gtcgaggatg gagccggtga tgatcagacc gatctcgtgg
     5221 tagttcagcg aggtgccgtc gaggttcgcg ctgccggccg tggcccacac gtcgtcgatg
     5281 atggcgactt tgctatggac atacacgggc atcacgtcgt aacgccgagg attccccgcg
     5341 ccgccgctgc ctgctttctc cgatcggctc cagagggtgt aggcgcccag gtgatgcccg
     5401 cctgttttgg cggcgctgcg gagttggttg acgttatcga tctggcgatc cggatagccg
     5461 ggcaggtcgg ggcgcaggtt caggaccagg atgatctgca ggcgcggctt ggtggtgtcc
     5521 ttcatcctcg cgatgagtgc atcgacgatt tcgggagagg tgaagtattg gttctcgatg
     5581 tagatgtacc gctgagcatt ggcgatggcg cgctcgtagg cttcgaggat gccggtctcg
     5641 ccgtagggca ggtcttcatc gctcgggttg acgcgcttga tcgacccgcc cggcagcgtg
     5701 cgcaggacct gcaccgaggc cacgtcgccg tcgggcgtga cgggcaggtc gggaaaggtt
     5761 ttaggcggag ggatgagcat cggtttgccg gtcgcattcc agatgctcgc gaacgtcttt
     5821 tcgatgtggg acaccgccgg gcccttgaga tcggtgttga cgtcgtgcat gagcgagccc
     5881 ttgtggcgcg cgtcccggat ggcatggttg gtgtcgctga agtagtactg tttcagcggc
     5941 gaccccatca ggatggcgtg gtccccatcc accacgatgg ccttggcatg cagcagggcg
     6001 aagcccttgt tgaacgcatt ggtgctcacg cccgccgtct gggcaaaaaa ctccgccacc
     6061 tcggtcacgg tgtcttccgc ggacagcggc aggttcgtca ccagcacgtt caccggtttt
     6121 ccacccttgt tcttgagcac ctcttccagc gaggtttccg ccgcctgctt gcatccagtg
     6181 gaaggaggat tcaagtgggt gaagctgctt ttgaacttgc tgaggaagcc gttgtcgacg
     6241 tcgaagaaca acgtcatcag gttgatggat tcggtggccc cctcggcggc attggtcacc
     6301 gcgggaaaca ttttggctcc gtcgatcaag tgggtgatgc gattgtggtg aaagagcgcg
     6361 accgggttgc ccacggcggg attcaacgtg gtgtgggtga caagccagcc ctcgatgttg
     6421 ttgcggtgga tggtgatggt gccgacctcg tggttcgagt cggtgacatc tttggcttcc
     6481 ggggtttcgt gcaggaggcg cgtgccgaag agtttcgggt cggcgtggat ggggccgaat
     6541 atctgggcga cgaggtcggg attccgatcg accttccagg tgcggtaggc gtccggcgag
     6601 taggaaatga gaaaggtgcc gtcgccttct gtcttgccga cgcccagcac atcgtcttcg
     6661 ctaaaaaaag gatcgaagtc gagcgcgcga acggtcaagc cggcgatgcc ggccccccgc
     6721 tcatcgatga tacgtccatg gatgctgatg ttggccatag gttccgtcag acgaccgaaa
     6781 cctacgccgc tcgcacgacc ctgtcaaccg tcaaggcacg tgggaatacg tgtatttatg
     6841 aggggggaca ggcatgctgt gtcatgttgc gacgatgtcg tgatggtctg tgtggtcacg
     6901 agctcacctg aagtaatcgg gtccacgatg aagcctcggg aacgcgatca cttcactgcc
     6961 ggctgtggca tgccatagcc atttgcccaa gccagatcga tcactcgctc attcgactgt
     7021 gagagcgaca attgctgatc gaacagcgtg ggatacgaac cgatcatcgt cgcctgaaag
     7081 tccgggtacc ggtctgcaga gacgccggtg agggtagcca acgacgcaag atattccccc
     7141 tggccgcggg ctatgtctgc gcggagattt tcgtgggtat gcatcgcgaa gacggtggcc
     7201 ttttgtttcg ccctgagcaa acggccgtcc gcgagcgagc ccggcgtcgt gctcgacgta
     7261 aattcggtcg tcggatcgag gaactctttg gtggctccgg atgtgccgtt ggtgagttga
     7321 gtcgtcgcgt cgaacggcgc cttcgtcaac tcgacggtgg cattcgtcat acagccgccg
     7381 agggtgagga gcaatccggg aatcagtgca aagcgtgtgg tgatcatatg aggcctcctg
     7441 ggttcgcccg atgtgtacac aagtgaagta ccacatcgag cgccagggcc gaccctagga
     7501 ggagccctag ttatgtgaca acttccgagt cgtggcgagt cgggcagcgt tcggggcagc
     7561 gcagggtacg tttccgtcct gcttgacagg accgcaagct cggcggaaaa tccgcagcct
     7621 ctgttcctgc tcaactggga agggacaaag aattgatcat tccgctgttg gtgatccctt
     7681 gaggcctact ctttttgtgt tagaaactct ttgcagttcg gggtcatgcc gaaacttagt
     7741 atttcgagtt gcatcctaga aaacttccga gtcgaaacgc tcgctgttct agggttcttt
     7801 atctgtgaag gatcatgcat tcgacctttg cgctgagcac ttcagtcagc gaccggatca
     7861 ttcgcctatt tctcgctcat gcgttttagc gagcaggaga ttagcttctg gttggggcgc
     7921 tcgatgactt tccgcagaac ttaatcacga agccgatacg ccatatcggt tttctctggt
     7981 catggtccct caaactgatg tgtggatgac agataccggt ggggcatcca aacagagaat
     8041 cttgcatcga ctgttcaacg gcttgggcat attcgcaatg cgtcggcctg ggcaatggtc
     8101 gtcttcaagg tctccacgtc caccggcttt tgcggaaacc gatgggccac gaatgcctga
     8161 gttacacgca tcggcgcgtt gctgatttgc ggcgttagcc caaaatccgt cttgcgggga
     8221 gaggtcacaa tcagtacaag tccagctact cctgcccacc tcgcgtggct tgtcgatacc
     8281 ggacaactca taactacggc tgaaggtcgc ccggtgcgag tcatggagct gagacacgcg
     8341 aaagatgatg ccgtcctttc ggcgtgggcg aagcacttcc gtaaccagta ttgccgcgac
     8401 gaccagatcg acgatctgtg caagggcacg aggctctctc gcgccgaata cctccggacg
     8461 atcaagttcc cggatcagag cgatgcgccg gggccaagca tccgcgcagg cgatttcgga
     8521 gaagtcctcg tcgcggacta tctcgagttt gctctggcct actggctgcc ccgcacgcgc
     8581 tacgacaaca agacggtgcg caacgagtcc acgaagggga ccgacatcat cggtttcaag
     8641 atcgttgctc ctggtcagga cgacccgaag gacgctctcg cgctcttcga ggccaaggcg
     8701 cagctttcgg gagacacgcc gagcgggcgg ctgaaagcag cggtggacgg atcggcgaag
     8761 gacgagattc gcaaggcaga gagcctcaac gcgatcaagc agcgcttcct tgaccgcggt
     8821 cgctctgcgg acgtgcagac ggtcgaacgg tttcagaatc cagaggatcg tccctacttg
     8881 gaggaatacg gcgcagtggc tctcctctcg tcctcggttt tcgacaagaa ggacgtcggt
     8941 gctacgacta tcgccagcca tccccacgcc agcaagttga cgctgattgt catccacggc
     9001 cctgacctga tgccgctggt tcacgaactc tatcggaggg ccgctgatga ggcctgagca
     9061 aagttcccgc gtgctgctcg ccatcacccg ctcgaaggcg aagatgttcg agtacggcct
     9121 tccggagaac gaacacatca agatctcccg agatccgtcg cgcctgctgc ggctcgccgt
     9181 ggggatgctc ggagatgttg ccgcagccgt gtcaaggaat ggaaccgatg cagcgcgtgt
     9241 ggccgacctc tcatcgtctc tacagttttc cgcgcgctac ttcgatgcgt acgcagcgag
     9301 caggctggcg gaagcaggcg atgcacatct cctcatcctc gcttctgcca cctactacct
     9361 gtgtgatctc cccggcagtt ccaacgttct cgtgtcgcgc attcctgacg tgccgaacct
     9421 cgacgctggc ggtttggagc accttctatg gtggcttctg cgtggcgact tttcttccgc
     9481 gatcgagcag accgagagcc cgaggtacag ggacgagatt cgccgcgtct cgcgcggcct
     9541 caaggcattc tacgatctag acactcaaga tgagcggcag ccacacgctg cgacagtgtc
     9601 gctgagggag actgcatacc gcgatggcac accgcgcgaa ctgctgttcg cggacacgtg
     9661 ttgcgcgatc gtcagccgca ggatcgaaaa ctccgcccga cgatcgttgc ctcagtactc
     9721 gggtctgccg atggagatct ggcgacctgc tctcgccaag aagtttttcg tgcgagagct
     9781 gtggcctgcg cagcgccttc tggggcagcg gagcatcttc gccggcgggt cggcggttgt
     9841 gcagatgcca acaagcgcgg gcaagagtcg cgcaaccgag ctcattatcc gcagtgcgtt
     9901 cttgagtggg cgagcgtcgc tcgctgtcgt cgtcggtccg tttcgagccc tctgccacga
     9961 gatccgacag agcctcgcgc gggcgttccg cggcgaggat gtaaacgtca acgagctgtc
    10021 ggacgttttc cagagagact tcgacttcga cgggctcgtt gctgggcacg cgattctggt
    10081 catgactccc gagaagctcg cctacgtgct acggcacctt ccagaacttg ccggtcgggt
    10141 cggtgtgctc gtcctcgatg agggccatca gttcgacacc gggccacgcg gcgtcacata
    10201 tgaactgctt gtcacttcgt tgaaggggat gatccccgca ggggcccaga aggtcctgat
    10261 ctcggcggtg atcaccaacg cttcggatat cgccaagtgg ctgaccgggg acgaaggcgc
    10321 ggtggtcgct ggggctgacc tcctgccgac cgagcgttcc atcgcgtttg ctagctggaa
    10381 aacgttgcta ggccagcttc acttcgtcgc gcccgagaat cctgacgaag acgagttctt
    10441 cgttccccgc gccatcaggt ccacatcctt ggcccttcgt ccgagagaga cgaaggagcg
    10501 ccggtttccc gagaagggcg acggccaatc ggtggccctg tacttggggc tcacattggt
    10561 tcgaaacggg gcgatagcaa tcttctgcgg gcgaaaggat acggcaacag gcctctgcga
    10621 ggcccttgtc gatgttgcag ctcgtggact tgcgatgcca atgcccgtcg aggtctcgga
    10681 tggtcccgag gtcgagcgcc tcgcctctct ctacgagcgg aatcttggaa ccggtgcggc
    10741 tgctgcgcgt gcggcgaaaa tcggggcgtt cacgcaccat gggaataccc cgcatggcgt
    10801 acgcctcgca gtcgagtacg cgatcaaaga ggggttcggc aagttcgtgc tctgcacgtc
    10861 caccttggcg cagggggtga acctcccaat ccggtatctc atcgtgacga cggcccagca
    10921 gggcggcgag aagatcaagg tccgcgactt ccacaacctc atggggcggg ctggtcgctc
    10981 gggcatgcac acggagggca gtgtcctttt cgcgaacccc gaggtgtacg acacgcgcgg
    11041 cgctgcggag agcaagtggc ccgagttcaa ggcattgctt caagtgagaa actcggaacc
    11101 gtgcgcgagc acactgttgt cggctttggg accgctgaag agcgatggag ggaaagtcac
    11161 gctgaccatc gaccctcttg atgtcgtgag ggactacgtt gccgatcaag atggcggtgg
    11221 cgcttgggtc gaccaggctg cgacacagct gactcgaaag tggttctcga cggagacgct
    11281 ccgaaggcag ctgacggaga ggcgcgacat ccttgcggcc gtggagagct tcctgctggc
    11341 tcactgggag gagggcggag acggcgaaag cttcggcgca ggcccagtcg gcgaggtggc
    11401 gaagcgaacg cttgcgtacc atctcgcatc agcggaacag cgcgcgcaac tgctgcagct
    11461 gttcgaactg ctcgcggcga acgtcaccgc gcgcggcggc gacgccgaga ggaggcgcgt
    11521 gtacgggagg acgctgttcg gcgtcgggga taccgtcgca ctcgagaagt gggtcgccga
    11581 caacctggaa cgcatcgctg gttgcgctga cctggacgaa ctcttcgaca tcgtgtggcc
    11641 gtgcgtcgca gcccgaatca agagcgacac gttccagaag tggcaaccag tagagacaag
    11701 agaagtcttc gcaaagcgtt ggatcgctgg cgactctttc gggtcacttc acgacagcat
    11761 gaccgccgcg agtgtccgga tcggcctcgg cgcgaagccg cgtaaaataa aagtcgagaa
    11821 catcgtcgag atgggggaga acgctctcgg cttcgatggt gcgcatttgc tcggggcgat
    11881 cgccgagctg ttcgagctcc tggcacccga ggatgagagc gagggcgaac cgctgcggct
    11941 gctacaggtc ctgcaaaagc gcctcaagta cggccttccg gcgggcgccc cgatccttct
    12001 atacgaggcg ggattcgccg atcgccccct cgccctcgaa ctcgcagcag cattcccgga
    12061 catctcgtcg cgtatcgaga tgagggacgc aatgcgcgaa gggcagcccg ccgtagaggc
    12121 ggttctgact cggtacccgc ggtacttcac tgaattgttg gagcgggtct cgggctagaa
    12181 cgatgagcac tggggtcctg tgagaggggc gggtctggca atcagacgcg ggatctacta
    12241 ggccgtttac atgacccata cggttcggat ctcttgtccc ggtgctgtct accatgccac
    12301 gagtcgtggg aatgtccggc agcccatcgt gatcgggggg cgagcccgcg cactgttcct
    12361 gagcggcttg cccacgtcat cgatcgcttt ggctgacgct gccacgcctg ctgcctggtg
    12421 gataaccacc attttgggtg tggagcaggt atcgggcgac ggcgggcgag gataccttgc
    12481 cgacctagcc gtgtagaaat tcggggtaga tcttttactg tccgcggcac tgagccggat
    12541 tgggcagatt gccccaaagg cctttcgaga aagtggaggg acgctgcttc gtgacaccgc
    12601 acgggttaac cccggccacg acatagt
//
LOCUS       DAORRQ010000087        12369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_245_length_12369_cov_54.225353, whole genome shotgun sequence.
ACCESSION   DAORRQ010000087 DAORRQ010000000
VERSION     DAORRQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12369
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245_length_12369_cov_54.225353"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK224_18345"
     CDS             complement(<1..278)
                     /locus_tag="PK224_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRB17763.1"
                     /translation="MELPDLKDDPHLKVLRPLVETYLAFWRMDSRHVRSLKLTPSQFD
                     VIATLGDTKGLTCAELSTATLVTKGTLTGVLDRLEDKELIRRTPVADDR"
     gene            380..1012
                     /locus_tag="PK224_18350"
     CDS             380..1012
                     /locus_tag="PK224_18350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB17764.1"
                     /translation="MPRHAQSGQANLTTILLLLGLVVSGVWVWKRLSPDMQDLIIDQA
                     IPLAALSLAIMAVAWILIGKIVKRRRRNQQRARLIKLFESQTASDKRLKLAFAVIEVN
                     EYRRVGLEAIASQFQELFVTTLKRALGDKQHQVRGMAASHLGVIGDDTAVAHLLAALE
                     DDHAYVRSSAALGLGRLRASAAKDKLAYVSKEDWDQTVRSRAREALERIR"
     gene            complement(1050..1883)
                     /locus_tag="PK224_18355"
     CDS             complement(1050..1883)
                     /locus_tag="PK224_18355"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRB17765.1"
                     /translation="MISSLMRWQELTRVWIGHWLSRPFHHLFNVMPGVEIGLSAPAIT
                     RLSLIHAPLAGRRAVHLSDLHLDRYRPRHRALVRTVADLRPDWIFITGDLLNVRDGLP
                     HVLRFLTELRRLAPVFVTLGNHDHYSGIRVDEFTEQFDRRKVTLLLNQVTFLPMDTGE
                     LAIVGLDDPSLHRADLRCIPSPRAGRFTLVLAHAPNILDQLAPEHHVDLTLCGHSHAG
                     QWRIPSIPTFWLPPGCHGRTNGMYTKGTHRLYVNRGIGWSVIPMRLNCPPEIVLLEWA
                     A"
     gene            complement(1880..2545)
                     /locus_tag="PK224_18360"
     CDS             complement(1880..2545)
                     /locus_tag="PK224_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17766.1"
                     /translation="MSIRFGHICCAVWLLCCAMTAGFAPSSMAATRLNGIDVFAQFTQ
                     HIRRQADTDKQAFAVASQCMTWFYKQQRHKPDPPAVQSIAWSRQTKSPWIFTDSSEQE
                     GSDCRTRYPGGIDAVRSDFQRTQAFLSLSLTFYEFALVGDGDDNSTYSSRELHDMLMA
                     LNLAAEPSLGADAHLRSLTSHFDSLHEARGMEALMTGMGKLYDQGYRVTSADKAHLNR
                     VME"
     gene            2667..3719
                     /locus_tag="PK224_18365"
     CDS             2667..3719
                     /locus_tag="PK224_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HRB17767.1"
                     /translation="MPPPSWLDDTVPYLTASVPALGGRIRSTPDDFCVEERPLYLPCG
                     EGEHLYIRVKKRGLSTPDLVLRLASQLHVKAQTIGVAGLKDAQAVTTQMLSLQGVKAE
                     TISALPTDERLLALEVLGRHRNRLRKGHHAGNQFRLVIRDVRERSEDDLQELFDELVR
                     RGVPNYFGPQRQGRSGTNFQLGAELLQDAARRNKMPRNKRIWFMNAYQSHVFNRIVAK
                     RIESIDRVFLGDWAMKSDNGACFPVEQPEVEQPRADRFEISPTGPLFGSRAPWATGVP
                     GDIEQAVVAELGTTPELLSKAGAECGFRGERRALRVRLNELSWSLEGTVLTLGFWLAP
                     GSYATSVLREVVKKSD"
     gene            3827..5071
                     /locus_tag="PK224_18370"
     CDS             3827..5071
                     /locus_tag="PK224_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HRB17768.1"
                     /translation="MTASQETVFLQGHIIDSLILAKVLDTILMMGGTFDLQDVKIGAT
                     RDASSQARIVVRAPSGRLLAEILRAIQPHGAAIEREADCRFEPAPAPGVFPEQFYATT
                     HLPTQIRLDGEWVEVEGMEMDLGICVDPAKARARTVPMGAVTAGDLIVTGREGIRVTP
                     LARPVERDVFGFMESVVSSERPHHPVIADIAQRMQKLREWHRQGRAGAKVLFAGGPAI
                     VHAGGREALAWLIESGYIQVLFCGNALAAHDMEAALYGTSLGYGLTAGRSVPHGHEHH
                     LRTINRIRTIGSIEQAVRSGVITGGIMAASVRCDVKLVMAGTIRDDGPLPGVLTDSMA
                     AQRAMRAEIPGVGLALLVASTLHAIATGNLLPATVPTVCVDVNPAVPTKLADRGSFQA
                     VGLVMDAASFLTELARELGWRP"
     gene            5068..5868
                     /locus_tag="PK224_18375"
     CDS             5068..5868
                     /locus_tag="PK224_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRB17769.1"
                     /translation="MSRLLVCPPDYFQIDYEINPWMRRENGVDPARAVRQWQALMTVL
                     EQDVGVGVERMQPVQGLPDLVFTANAGVVVGRRALVSRFRYPERQREEAHFARWFGER
                     GYEVLTLDPSLYFEGAGDLLGFPDTWFGGYRQRSDIRAFPKLSEIFQREIIPLELIDS
                     RFYHLDTCFCPLSGGDLLYLPSAFDSYGQAAIAERVPEDRRLAVPEEEALRFACNAVC
                     VGKQVVIPAGCPLTTKLLEDRGYRAHPVQLDEFMKSGGAAKCLTLALD"
     gene            complement(6137..6496)
                     /locus_tag="PK224_18380"
     CDS             complement(6137..6496)
                     /locus_tag="PK224_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRB17770.1"
                     /translation="MKPCPACGRGLPEINRYCTHCGAGVSPDTSRRTAATPPGPAKEQ
                     LNLNILYGMVTVLIVSLLMPPWETPPSQPAAFLGFHFILSPPQPDALVSRLLLTIELT
                     TTAIAGLYLSFLFRTRQ"
     gene            6678..7703
                     /locus_tag="PK224_18385"
     CDS             6678..7703
                     /locus_tag="PK224_18385"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRB17771.1"
                     /translation="MEQPAIKHVAVIGAGAWGTALARHLAEKQVPVCLWAHEPEVVQA
                     IQLRRENTVYLPGVLLPPTVSATNVLSDAIAGADCLIFAVPSHVARPVLSRVAVLLPQ
                     PIPLVSATKGIEEETLALVTQVMQSVLPSHMQGSLLVLSGPSFASEVSRGKPTALCLA
                     GQDAGVVKAIQSLFMTPNFRVYADDDLTGVQLGGALKNVMALAAGVVDGLELGHNARA
                     ALITRGLAEMIRLGVAMGADPRTFYGLSGVGDLILTCTGPLSRNHSVGVRLGKGERLE
                     GILASMQAVAEGVRTAKAALGLALRCGVDMPIVQEVNAVLFADKSCRQAVGDLMEREA
                     KGEKSPS"
     gene            7700..8482
                     /gene="larB"
                     /locus_tag="PK224_18390"
     CDS             7700..8482
                     /gene="larB"
                     /locus_tag="PK224_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRB17772.1"
                     /translation="MNQDQLQALLTKVQQGALAVPDALHRLRTLPYENLGFASLDHHR
                     ALRQGFPEVIFCEGKTVSQVVAIAKTLLRKNNALLATRVEPSVARALLRVSKRAVYHE
                     AARVVAIAPPKLVRRGAVLIVTAGTADIPVAEEARVTADIMGSKTDTLYDVGVAGLHR
                     LLGQQERLHDARALIVVAGMDGVLPSVVGGLVRQPVIAVPTSRGYGAHFGGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINMVGEVKGAVPVGKND"
     gene            complement(8709..9281)
                     /locus_tag="PK224_18395"
     CDS             complement(8709..9281)
                     /locus_tag="PK224_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17773.1"
                     /translation="MINATPFSSTIVLRALHAVRLGLLFLLGAALLLGGHPMDASAKT
                     KGKSSVPRPEPDLKILSLHAAPMPYRPQDGPFHFIATVQLPKEVDEQLMLEVSALVTS
                     SSMTSLRFLSIRQPVNEHAQTATSAAGDTPQKHVQVDLVWDGLDHNKQQVPAGSYEYE
                     VRVKLLSNGDKGQRTQMLAWPKRGKVVVKE"
     gene            9451..10659
                     /gene="larC"
                     /locus_tag="PK224_18400"
     CDS             9451..10659
                     /gene="larC"
                     /locus_tag="PK224_18400"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRB17774.1"
                     /translation="MATHLHFDCFSGISGDMTLGALVDAGLPFKDLVRGLALLRIEGF
                     RLTRKRVERGALTATKVDVLVEKGFRAPLTLAQIKRILLKSGLPPVVKEKSQAVFDVL
                     AHAEGKAHGVEPSHVHFHEVGVIDSFVDVVGGVLGLHLLGVQRVTASAINVGSGTLMS
                     AHGSLPVPGPAVAALAVGLPIYAGGPERELATPTGVALVRTLATEFGRLPHMQVRRVG
                     YGAGTADPAHWPNVLRVFVGEEPAAAGSVETIVELQTNIDDLNPQVYETVFDRVFAAG
                     AVDATLAPVTMKKGRPGNVLSVLAPQEKVEAVLAVLFADTTALGVRTHEVQRRVLPRR
                     FVPVQVHGHEVSIKVADSQPGRSKAAPEYEDCKRIAEQSGRPVKDILEEAMQAYRRTA
                     GHAKKGKGSR"
     gene            10718..11731
                     /locus_tag="PK224_18405"
     CDS             10718..11731
                     /locus_tag="PK224_18405"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17775.1"
                     /translation="MTILLYVGLFVASVVVTLAGCHLFTNGIEWLGKRLNISEGAVGS
                     VFAAVGTTLPETSIPIIAIFFGAGREQMEVGLGAILGAPFMLSTLVLPILALLLLLYA
                     KLGKRTATFKLNYGEVRVDISFFLISYALALTCAVIPSKLFHVVVAAVLLGMYVYYMK
                     LKFSAEDEEGEGGSELEPLLFARRSSRPSYLVIGAQGVVGLLGLVGGAHLFVTAANSI
                     SAEMQVSPLILALLIAPLATELPEMSNSFLWLYRKKDRLAVGNVTGAMVFQGSIPVSV
                     GLLGTEWTLGTTALATMVLAVLAMSLSLLQAVWSGHWRPWLLSGSALLYLGYTLFLYA
                     HGS"
     gene            11772..>12369
                     /locus_tag="PK224_18410"
     CDS             11772..>12369
                     /locus_tag="PK224_18410"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRB17776.1"
                     /translation="MGDPAGIGPEVIAKALADRALTRLCTPVVVGSRHVMERTIAWLK
                     LPLQVVDFNPANGQRLKAGQVAVADPLESPLPKFRMGAATAVTGAASIAFIKEAVKLA
                     QAGSLGGIVTAPINKEAMNMAGFHYPGHTELLADLTKTTEFGMMIVGGPLKIMFTTTH
                     VAINALSPLLTTERISKAIRLAHLGLTKYFGIARPRIGV"
BASE COUNT         2269 a         3446 c         4147 g         2507 t
ORIGIN      
        1 cggtcatcgg cgaccggcgt gcgtcgaatc agctccttat cctccaggcg gtccagcacg
       61 ccggtcaggg tccccttggt caccaaggtg gcggtagaga gttcggcgca ggtcagtcct
      121 ttggtatcgc ccaacgtggc aatcacgtcg aattgggagg gggtcagctt caacgaacgc
      181 acgtgacggc tatccatgcg ccaaaaggcg aggtaggtct cgaccaacgg gcgcaggact
      241 ttgaggtgtg gatcgtcttt gaggtcgggt aactccattg cgcgcgcagc ctagtcatcc
      301 catcgggtag ctgtcaagag tcggccgccc aaggtcgcgt tgacgccgca cagatcgtcc
      361 ccctatcatg tcgcctgcca tgcctcgtca cgcgcaatcc ggccaagcca atctgaccac
      421 gattctcctc ctgctgggtc ttgttgtatc cggcgtttgg gtgtggaagc ggttgtcgcc
      481 ggacatgcag gatctcatca tcgaccaggc gattcccctg gccgccctgt cgcttgcgat
      541 catggcggtc gcgtggatcc tcattggaaa gatcgtcaaa cgccgtcgaa ggaatcagca
      601 gcgggcgcga ttgatcaagt tattcgagag ccagaccgcc tccgacaaac gactcaagct
      661 ggcattcgcg gtgatcgagg tgaacgaata ccggcgggtc gggctcgaag cgatcgcatc
      721 gcagttccag gaattatttg tgacgaccct caagcgggcg ttgggagaca aacaacatca
      781 ggtacggggg atggcggcaa gccacctggg agtgatcggc gacgacacgg cggtggccca
      841 tctcctggcg gccttggaag atgaccatgc ctatgtgcga tccagcgcgg cattgggcct
      901 cggtcggttg cgagccagcg cggccaagga caagttggcc tatgtcagca aggaagactg
      961 ggatcaaacg gtgcggagtc gggcacgcga ggcgttggaa cgtattcgat gagaaggcag
     1021 cgtagcgggc agagcagatg gtcgaacgat tacgcggccc attcgagcag aacgatttca
     1081 ggcgggcaat tcaaccgcat ggggatgacc gaccatccga tcccacggtt cacgtagagc
     1141 cggtgggtgc ctttggtata catgccgttt gtccgtccat ggcagcccgg cggcaaccag
     1201 aacgtgggaa tggaggggat ccgccactgc ccggcatggc tgtgtccgca gagggtcaag
     1261 tccacgtggt gttcaggcgc gagctggtcc aggatattcg gcgcatgcgc cagcacgaga
     1321 gtgaatcgtc cagcccgcgg tgagggaatg caacggagat ctgcacggtg cagtgacgga
     1381 tcgtcaagcc cgacgatggc cagttcccct gtgtccatcg gcaggaacgt gacttggttg
     1441 agcaggagcg tgaccttgcg tcggtcgaat tgctcggtga attcgtccac ccggatgcca
     1501 ctgtagtggt cgtggttacc gagcgtgacg aagaccggtg ccagacgccg gagttccgtc
     1561 agaaagcgaa gcacatgcgg caggccgtcg cgaacgttca ggaggtcgcc ggtgataaag
     1621 atccaatccg gcctcaagtc ggcgacggtg cggacgagcg cccggtgtct ggggcgatag
     1681 cggtccagat ggaggtcgct gagatgcacg gctcgccggc ccgccagcgg agcatggatg
     1741 agggagaggc gggtgatggc cggcgcggac agcccgattt cgacccccgg catcacattg
     1801 aacagatgat gaaaggggcg gctcagccaa tggccgatcc agacgcgcgt cagctcctgc
     1861 caccgcatca aggaggagat cattccatca ctcgattgag atgcgccttg tctgcgctgg
     1921 tgacgcggta gccctggtca tacagtttgc ccatgccggt catgagcgct tccatgcccc
     1981 gggcttcgtg tagggaatcg aagtgtgaag tgagggaacg gagatgggca tccgctccaa
     2041 gcgatggttc agcggccagg ttcagcgcca tcagcatgtc gtgcagttca cggctgctgt
     2101 aggtcgagtt gtcgtcgccg tccccgacca gggcgaattc gtaaaaggtc aggctcagcg
     2161 agagaaacgc ctgggtgcgt tggaagtcgc tgcgaacggc gtcgatcccg cccgggtaac
     2221 gcgtgcggca gtcggagcct tcctgctctg aagaatcagt gaagatccat ggtgactttg
     2281 tctgtcggct ccaggcaatg ctctgcacgg caggcgggtc gggtttgtgc cgctgctgct
     2341 tgtagaacca ggtcatgcat tgactggcca cagcaaaggc ctgcttgtcg gtgtcagcct
     2401 ggcggcggat gtgctgggtg aattgggcga agacatcgat gccgttcaac cgggtcgcgg
     2461 ccatggatga gggggcgaag ccggccgtca tcgcgcagca cagcagccag acggcgcagc
     2521 agatatgacc aaatcggatc gacatcgggt tcgtctacag ataattgcag gagtcgtgga
     2581 cggcctcatt cgagtataac acgggtagtg cctgtgccta cccaaatgac cgcaattgtg
     2641 tccgatgaag cacgagaagg aaacctatgc cgcctccgtc ctggctcgat gacacggtgc
     2701 cctatttgac cgcctcggtt cccgccctgg gcggccggat ccgcagtact ccggatgatt
     2761 tttgtgtgga ggaacggcca ctgtatttgc cttgcggcga aggtgagcat ctgtatatcc
     2821 gggtaaaaaa acgggggctg tccacgccgg atctcgtctt gcgacttgcc tcccagctcc
     2881 acgtgaaagc ccagaccatc ggggtggccg ggctgaaaga tgcgcaggcc gtgacgacgc
     2941 agatgttgtc gttgcaggga gtgaaggcag aaaccatctc ggcattaccc accgacgagc
     3001 ggttgctggc gctggaagtc ctggggcgcc atcgcaaccg gcttcgtaag gggcatcatg
     3061 cgggcaacca gtttcgtctg gtgatccggg atgttcggga gcgcagcgaa gacgacctgc
     3121 aagagttgtt cgatgaactc gtgcggcggg gcgtccccaa ctatttcggt ccgcaacggc
     3181 aagggcgatc ggggacgaac tttcaattgg gtgccgagtt gctccaggac gcggcccgtc
     3241 ggaacaagat gcctcgcaac aaacgcatct ggttcatgaa tgcctaccag tcccatgtgt
     3301 tcaatcgcat cgtcgccaaa cggatcgaga gcatcgaccg ggtgtttctg ggcgactggg
     3361 cgatgaaatc ggacaacggt gcctgttttc ctgtagagca gccggaggtc gagcagccgc
     3421 gtgccgaccg gtttgagatc agtccgaccg gaccactctt tggctcccgc gcaccctggg
     3481 cgacgggggt cccgggtgac attgaacagg ccgtggtggc tgagctgggc accacgccgg
     3541 agcttctgtc gaaagccggc gccgaatgtg gattccgtgg cgaacggcga gccctgcgcg
     3601 tccgtctcaa cgagttgtcc tggtcgctgg agggcactgt gctgaccctt ggcttctggc
     3661 tcgctcccgg ttcctacgcc acgagcgtcc tgcgggaagt ggtgaagaaa agcgactgac
     3721 cctcgcgcct cattccttcc ggcagatggt agaatttaca gagtcaggca tgttcccccc
     3781 ggacgccttc cgctccggcg ccggcacgag tcgtgagagg tccgtcatga ccgcatcgca
     3841 agaaaccgtg ttcctgcagg ggcacattat cgattcgttg atcctggcca aagtcctgga
     3901 tacgattctg atgatgggcg gtactttcga tctgcaggac gtcaagatcg gcgcgaccag
     3961 ggatgcctct tcccaggcgc ggattgtggt gcgagccccg tccggccgcc tgttggccga
     4021 aattctgcgg gccatccaac ctcacggtgc cgccattgag cgtgaagcgg actgtcgctt
     4081 cgagccggca ccggctccgg gagtgtttcc cgaacagttc tatgcgacga cacatttgcc
     4141 gacccagatt cgtctggatg gcgagtgggt ggaggtggag ggcatggaaa tggaccttgg
     4201 catctgtgtc gatccggcca aggcccgcgc caggaccgtt cccatggggg cggtgacagc
     4261 cggcgacctc atcgtgaccg ggcgggaggg aatcagggtt acacccctcg ctcgtcctgt
     4321 cgagcgtgac gtgttcggat ttatggaatc tgtggtgtcg tcggagcggc cgcatcaccc
     4381 tgtcattgcc gacatcgctc agcgcatgca gaagttgcgc gaatggcatc gacaggggcg
     4441 cgccggggcc aaggtgctct ttgccggcgg cccagcgatc gtgcacgccg gggggcgaga
     4501 ggccctggcg tggttgatcg agtccgggta catacaggtc ttgttctgcg gcaatgcctt
     4561 ggctgcgcac gatatggaag ccgcgctcta cggcacgtcg ctcgggtatg ggctgacggc
     4621 cggccgttcg gtgccgcacg ggcatgagca tcatctgcgc accatcaatc gcattcgtac
     4681 catcggcagc atcgagcagg ctgtgcggtc gggcgtcatc accggcggaa tcatggcggc
     4741 ctctgtgcgg tgcgatgtga agctggtgat ggcgggcacc attcgcgacg acggtccctt
     4801 gcccggggtg ctgacggact ccatggcggc gcagcgggcc atgcgcgcgg aaattccagg
     4861 ggttggactt gccttattgg ttgcatccac gctgcatgcg atcgcgaccg gcaatttgtt
     4921 gccggccacc gtgcccacgg tgtgcgtcga tgtgaatccg gcggtgccga cgaaattggc
     4981 tgaccgtggc agtttccaag ccgtcggcct tgtcatggat gccgcctcct ttctcacgga
     5041 actcgcgcgt gaactcgggt ggcgtccatg agccgcctgc tcgtgtgccc ccccgattat
     5101 tttcagatcg attacgagat caatccctgg atgcggcggg aaaacggtgt ggatccagcg
     5161 cgtgccgtcc ggcaatggca ggcgctgatg accgtgctgg agcaggatgt cggtgtcggg
     5221 gttgagcgga tgcagcctgt tcaggggctg cccgacctgg tctttacggc caatgcggga
     5281 gtcgtcgtcg gtcgtcgcgc gctggtgagc cgctttcgct atccggagcg acagcgggag
     5341 gaagcgcatt ttgcgcgatg gttcggtgaa cgcggatatg aagtattgac gctcgacccg
     5401 tccctgtatt tcgaaggcgc cggcgatctg ctgggatttc cggacacctg gttcggcgga
     5461 taccgccagc ggtcggatat ccgggcgttt ccgaagttaa gcgaaatctt ccagcgggaa
     5521 atcattcctc tcgaattgat cgacagccgc ttctatcacc tcgacacctg tttttgtccg
     5581 ctcagcggcg gcgacttgct ctacctgccc tcggcgttcg actcctacgg tcaggcggcc
     5641 atcgcggaac gggtcccgga ggatcgtcgg ctcgcggtgc ctgaagaaga agcgctcaga
     5701 tttgcctgca atgcggtctg tgtcggcaaa caggtcgtga tccccgccgg ttgtccgctc
     5761 acgacgaagc tcctggaaga tcgaggctat cgcgcccatc cagttcagtt ggacgaattt
     5821 atgaagtcgg gtggcgcagc gaaatgcctg actctcgccc tggattaatc cggagtattc
     5881 atgaatgccg tcgcgaggcg ttcgagacgg aagcccgccg aggttactcg cacgtgaggg
     5941 cgacgcaggc gtgctcgccg tccgtcgagg gggccgaacg agagaagcct tgccgggtga
     6001 gaggatcgga cgccggagca ggtattcatg aatagtccgg gttaagcaga aacggctacc
     6061 gtcattggtc aagcgcaatg gtgactggaa ggacagtccg ttcacaggtg acgcgtgacg
     6121 actgacgatg gaccggctac tgtctcgtgc ggaatagaaa cgacagatac aggccggcga
     6181 tggcggtggt ggtgagctca atcgtgagca gcagccggct gacgagggca tccggttgcg
     6241 gcggggagag gatgaaatga aacccgagaa aggccgccgg ctgtgaaggt ggcgtttccc
     6301 agggcggcat gagtagggag acgatcagga cggtcaccat gccgtacagg atattcaggt
     6361 tcagctgttc cttggccggt ccgggaggag tcgccgccgt tctccggctg gtgtcagggc
     6421 taacacctgc tccgcagtgg gtgcaatagc ggttgatttc cggtaggcca cgtccgcagg
     6481 caggacaggg tttcatgaca gtcgttccag atgaagtctg cagcctaacg cgacggcggc
     6541 aggatttcca gtggcgagat agggcatcgc ttcgttgaca gctttcaaag ccccttccta
     6601 taatccgcgt ccatgaagtc tgcattctgc attccagctg ccggcccgct ccgccacgtc
     6661 atggtggtgt ggcagtgatg gagcaaccgg ccatcaagca tgtggcagtc atcggcgccg
     6721 gggcctgggg gacggcgttg gcccggcatc tggccgagaa acaggtgccc gtctgtttgt
     6781 gggcccatga gccggaagtc gttcaggcga tccagctcag gcgcgaaaat acggtgtacc
     6841 ttccgggcgt gttgcttccg cccaccgtat cggcaacgaa tgtcttatcc gacgccatcg
     6901 caggcgcgga ttgcctgatt tttgcggtgc cctcgcatgt agcgagaccg gtgctgagcc
     6961 gggttgcagt cttattgccg cagcccattc cgctggtcag tgcgaccaag gggatcgaag
     7021 aagagacgct tgcgctcgtg acgcaggtca tgcagagcgt gttgccctcg cacatgcagg
     7081 gttccttgtt ggtcctgtcg ggaccgagtt ttgccagtga ggtcagccgc gggaaaccga
     7141 cggcgctgtg tctcgccggg caggatgccg gtgtcgtcaa ggcgatccag tcgctgttca
     7201 tgacgccgaa ctttcgcgtc tatgcggacg atgacttgac cggtgtgcaa ctcggcggcg
     7261 ccttgaaaaa cgtgatggcc ctggctgccg gtgtcgtgga tgggttggaa ctcgggcata
     7321 acgcccgcgc cgcgctcatt acccgcggcc tggccgaaat gatccggctg ggtgtggcga
     7381 tgggtgctga tccacggact ttctatggcc tctcaggggt cggtgatctc attctgacct
     7441 gcaccggtcc cttgagccgc aatcattcag tcggggtgcg gctggggaaa ggcgaacgct
     7501 tggagggaat tctcgcgagc atgcaggcgg tggcggaagg cgtgcgcacg gccaaggcgg
     7561 cattggggct cgccctgcgc tgtggggtgg atatgccgat cgtgcaggaa gtaaacgcgg
     7621 tgctctttgc cgacaagtcc tgtcgtcagg cggtcggtga tctcatggag cgcgaagcca
     7681 agggggagaa gagcccgtca tgaatcagga ccagctccag gcgttgctca cgaaggttca
     7741 gcagggcgct cttgcggtgc cggatgcgct tcaccggtta cgcaccctgc cgtatgaaaa
     7801 cctgggattt gcctcgctcg atcaccatcg cgcgctgcgc caggggtttc cggaagtcat
     7861 tttttgcgaa ggcaagacgg tatcacaggt ggttgccatc gcgaagacgc tcttgaggaa
     7921 gaacaacgcc ttgctggcga cgcgggtgga accctcggtt gcgcgggcgc tcctgcgcgt
     7981 gagcaagcgg gcggtctacc acgaagcggc gcgggtcgtc gccattgcgc cgcccaagtt
     8041 ggtgcgccgg ggggccgtat tgatcgtcac cgccggcacc gccgatattc ccgtggcgga
     8101 agaagcgcgt gtgacggccg atatcatggg gagcaagacc gacacgttgt acgatgtggg
     8161 agtggccggc ttgcatcgcc tgctggggca gcaggaacgg ttgcatgatg cccgggccct
     8221 catcgtggtg gccggcatgg atggcgtgct tccaagcgtg gtcggcggac tggttcggca
     8281 accggtgatt gcggtgccga cgagccgggg ctacggcgca cacttcggcg ggttggccgc
     8341 attgttgacc atgttgaatt cctgcgcggc cggagtcgga gtcatgaaca tcgacaacgg
     8401 tttcggagcc ggttgcctcg cgcatcgaat caacatggtg ggtgaagtaa aaggtgcggt
     8461 tccagtaggc aaaaatgatt gagtctgcgg cgtttcctcg gccgactagc aggccgttaa
     8521 aaagccattt tgctgtctct gaaaaaagtg atgcgtgagt tgggagagat attcagaaca
     8581 ccagcaggat ggtcaaaaag gtcgtccagc gcggccgcag cgatgcgagg aggtgaagcg
     8641 tacccttgcg gtacgttgag cctcggagcg acgcgagaac aaagctggcg gactttttca
     8701 acatcctgct attccttcac gacaaccttg ccgcgtttgg gccatgccaa catctgcgtg
     8761 cgttggccct tgtctccgtt actcagtaat ttgacccgca cttcatattc ataggacccg
     8821 gccggcactt gctgcttgtt gtggtccagt ccgtcccaca cgagatccac ctgcacatgt
     8881 ttctgcggcg tgtccccagc ggcgctggtg gctgtctgcg catgttcatt gaccggctgc
     8941 cggatcgaca ggaagcggag cgaggtcatg gaggaggagg tgacaagagc ggacacctcc
     9001 agcatcaatt gttcatcgac ttccttgggc agttgcacgg tcgcgatgaa atgaaagggg
     9061 ccgtcctgcg ggcggtaggg catcggcgcg gcatgcagcg agagaatttt caggtccggc
     9121 tccgggcgcg ggacgctgct tttgcccttg gttttggccg acgcatccat gggatgtcct
     9181 cccagcagaa gggccgcacc taagagaaac aggaggccga gtcgtacggc atgcaatgcg
     9241 cgaaggacaa tcgtagagct gaatggtgtg gcattgatca tggtgttgaa gtcgcagcgt
     9301 gtgggccggt ggtccgatgg aatgcgtctc ttgattgggg ataacacagc atcgaagggc
     9361 tgtcaatgaa cgagcggaat gattgctgtc tggatgcggc tccggtaaga tgccgctcgc
     9421 tcgcagtgga tgatgaatcg taggagtgac gtggccacac atcttcattt cgactgtttc
     9481 tccggcatca gcggtgacat gaccttgggc gcgctggtcg atgcgggatt gccgtttaaa
     9541 gatctcgtgc gcgggctggc cttgttgcgg atcgaggggt ttcgcctgac gcggaaacgg
     9601 gtcgagcgcg gggccctcac tgccactaaa gtcgatgtgc tggtcgagaa gggctttcgc
     9661 gccccgttaa ccctggcgca gatcaaacgg attctactga agagcggcct gcccccggtg
     9721 gtgaaagaaa agagccaggc cgtgttcgat gtgctggctc acgctgaggg gaaggcccat
     9781 ggcgtcgaac cctcgcatgt gcattttcac gaagtcggcg tgatcgattc gtttgtcgat
     9841 gtggtcggcg gggtcctggg gctccatctc ctgggggtgc agcgcgtgac ggcctccgcg
     9901 atcaatgtgg gatcgggcac cctcatgtcg gcacacggat cgttgcctgt gccgggaccg
     9961 gccgtggccg cgctggcggt cggcctgcct atctatgccg ggggtcctga gcgggaactg
    10021 gcgactccga ccggagtcgc gctggtgcga accctggcga cagaattcgg tcgcttgcct
    10081 catatgcagg tgcgccgggt ggggtatggg gcaggaaccg ccgatccggc gcattggccg
    10141 aatgtgttac gagtgttcgt cggtgaagaa ccggccgccg caggttcggt ggaaaccatc
    10201 gttgaacttc agacgaacat cgacgacttg aatccgcagg tctacgagac cgtgttcgat
    10261 cgggtgtttg ctgcgggagc cgtggatgcg accttggcac cggtcacgat gaaaaagggc
    10321 cggcccggga atgtcctctc cgtgctggct ccccaggaga aggtagaggc cgtgctggcc
    10381 gtgttgttcg ccgacacgac ggcgctcggg gtcaggaccc atgaggtgca gcgacgggtg
    10441 ctgccacgac gatttgtccc ggtacaggtc catggccacg aggtgtctat caaggtggcg
    10501 gactcccaac cgggccggag caaagccgct cctgagtatg aggattgtaa gcggattgcc
    10561 gaacagagcg gccgtccggt gaaagacatt ttggaagagg ccatgcaggc ctatcggcgc
    10621 acggcaggac atgcgaaaaa gggaaaaggg agcaggtgaa cacagaaact gccgccgttg
    10681 tcttcttgtt tgaggtgtct tcctcgcgag gcctccagtg acgatcctgc tctatgtggg
    10741 attgtttgtg gcctcggtcg tcgtcaccct cgccgggtgc catctcttta cgaacggcat
    10801 tgaatggctc ggcaagcgtc tcaacatttc tgaaggcgcg gtcgggagcg tcttcgcggc
    10861 ggtcgggacg acccttccgg agacgtcgat tccgatcatc gcgatttttt tcggggccgg
    10921 gcgagagcag atggaggttg gtctgggggc cattctcggg gcgccgttca tgctcagcac
    10981 gctggtcctg cctattctag cgctgctgtt gctgctctac gcgaagctcg gtaagcggac
    11041 ggcgacgttc aagctcaatt acggcgaagt ccgcgtcgat atcagcttct tcctcatcag
    11101 ttatgcgctg gcgttgacct gcgcggtcat cccctccaag ctctttcacg tggtggtcgc
    11161 ggcagtgctg ttgggcatgt acgtctatta catgaagctc aagttctcgg ccgaagacga
    11221 agagggtgag ggggggagtg aactggagcc gctgttgttc gcacgccggt cctcccgtcc
    11281 gtcgtatctg gtgatcggcg cgcagggagt cgtcggattg cttggcctgg tcggtggggc
    11341 ccatctgttc gtgacggccg cgaattccat ttcagcggaa atgcaggtct cgccgctcat
    11401 cctcgcgttg ctgatcgctc cgctggccac tgagttgccg gagatgtcga acagttttct
    11461 ctggttgtat cggaagaagg atcggctggc cgttggaaat gtcactggtg cgatggtgtt
    11521 tcagggctcg attcccgtct ctgtcgggtt gctgggcacc gaatggacgc tggggacgac
    11581 ggcgctggcg accatggtgc tcgccgtgct cgctatgagc ttgagtctac tgcaggcagt
    11641 ctggtccggg cattggcgac cctggttgct cagcggtagc gcgctgctgt accttgggta
    11701 cacactgttc ttgtacgcac atggctccta aacgcacgac caaaacagcg cgtcctgtcc
    11761 taggtctcac aatgggagat ccggccggca tcggaccgga agtcatcgcc aaggccttgg
    11821 ccgatagggc tctgacgcgt ctctgcactc ctgttgtcgt cggctcccgt cacgtcatgg
    11881 aacggacgat cgcctggttg aaattgccct tgcaggtggt ggacttcaat ccggcgaacg
    11941 gccagcggtt gaaggcgggg caggtggccg tggcagaccc gctggaaagt ccgttgccga
    12001 aatttcgcat gggtgccgct accgcggtga ccggtgcagc ctccatcgca ttcatcaagg
    12061 aagcggtgaa gctggcgcag gcggggagcc tgggtgggat cgtcacggct cccatcaaca
    12121 aagaagccat gaacatggcc ggttttcact acccggggca cacggaactg ctggcagatc
    12181 tgaccaagac cacagagttc gggatgatga ttgtcggcgg gccgctcaaa atcatgttca
    12241 ccaccaccca tgtggcgatc aatgccttgt ccccgctgct gaccacggaa cggatcagca
    12301 aggccatccg cctcgcgcat cttgggttga cgaagtattt cggcattgcg cgtcccagga
    12361 tcggcgtgg
//
LOCUS       DAORRQ010000088        12343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_247_length_12343_cov_51.126465, whole genome shotgun sequence.
ACCESSION   DAORRQ010000088 DAORRQ010000000
VERSION     DAORRQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_247_length_12343_cov_51.126465"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK224_18415"
     CDS             <1..198
                     /locus_tag="PK224_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRB17777.1"
                     /translation="VKAYGGETIKAGSIIVRQRGTKFFPGLNVGLGRDHTLYAYVSGV
                     VKFEGGRGRQKVSVYPVTAKA"
     gene            324..1355
                     /gene="obgE"
                     /locus_tag="PK224_18420"
     CDS             324..1355
                     /gene="obgE"
                     /locus_tag="PK224_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRB17778.1"
                     /translation="MSTFVDQVQILVKAGHGGNGACSFRREKFVPRGGPDGGDGGDGG
                     SVVVEATTRLSTLLDLRYQKHYEAEKGETGGGSNCHGRRGADVRIPVPVGTMVFDENT
                     QELLADLTKDGETCVIAKGGQGGRGNTQFATSTNRVPTQFEPGTPGEERLLRLDLKLL
                     ADVGLVGYPNAGKSTLIAAVSAARPKIADYPFTTLTPNLGVVRWTGEQTFVIADIPGL
                     IEGAHEGKGLGFQFLRHIERTSLLLHVIDISEWATEDPVASLEIMRHELTAYDDTLAA
                     RPFAVVGTKLDVKGEGERIERLRKYCQRRKIPFFAISAATREGLDECIRYIGQQVELL
                     RKTPCETNS"
     gene            1337..2458
                     /gene="proB"
                     /locus_tag="PK224_18425"
     CDS             1337..2458
                     /gene="proB"
                     /locus_tag="PK224_18425"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRB17779.1"
                     /translation="MRDELLRQAKRVVIKIGSSLIASRETGLSAERIERLAGEIAEVR
                     AQGREVLVVSSGAVVSGIKKLGLREYPKSLPLKQAAAAVGQSLLMWAYEKAFERLTLR
                     VAQVLLTHEDLADRRRFLNARHTLTTLIKFGVIPIINENDTVAVEEIRVGDNDTLAAQ
                     VAHLVDADLLIILSDVDGLFTVDPRKDPTAALIPLIPEITEDIEQRAGMSSTFEGTGG
                     MATKVRAAKKVGEYGVATLILNGTQTRLLPNVLSGQPGGSLFLARERRMNSRKHWIAF
                     TLRPRGHVQLDQGAVEALVRNGKSLLASGILDITGHFDAGDPVTCAGPDGKECAKGLV
                     NFSSEILTRIKGLKTADIQKIPGLQEYEEVIHRDNLVIL"
     gene            2561..3241
                     /gene="nadD"
                     /locus_tag="PK224_18430"
     CDS             2561..3241
                     /gene="nadD"
                     /locus_tag="PK224_18430"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRB17780.1"
                     /translation="MRLGLLGGSFNPIHRCHLSIARSARQLLQLDRVLFIPTGDPPHK
                     QPGTLATASHRHRMVQLAIEDTPEFALTDLEIRRSGKSYSIDTVRAIQQEYGPDTVLF
                     FIIGLDAFLDLPSWKEADTLLKSCHFVVISRPSTSFQAMAAIPLFRDVPQTTLTALDE
                     VRQERADVAITNGQALTFLRLPPCDVSASEIRARLRSGASLANLLPTPVESYILREGL
                     YREDSERL"
     gene            3228..3650
                     /gene="rsfS"
                     /locus_tag="PK224_18435"
     CDS             3228..3650
                     /gene="rsfS"
                     /locus_tag="PK224_18435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRB17781.1"
                     /translation="MSVSESKAKALAVASAILDKKATDVLILHIATLTSVADYLVIGS
                     GESERQARAMADHVSDVLTAQGHRPLSTEGASSAKWVVMDFGDVIVHIFQKDIREHYA
                     LERLWGDAGQVRLPEKRAVKAAPPTRTATRPARTRKRV"
     gene            3652..5052
                     /locus_tag="PK224_18440"
     CDS             3652..5052
                     /locus_tag="PK224_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17782.1"
                     /translation="MFRLLSTIFLVSVGIFLYSYFRELNPGTITVRTSPDALFELSPV
                     SLVLFSMALGATLVALIVTIKETSHVFMNWRTNRLVRRKEKVDALHRDGTHAFMSKRT
                     ADAVNLFERALVIDPNRTDSLLWLGNIYRSESNFTEAIRLHQQAHRIEERNVEVLLEL
                     AKDLEGARRYEDALQTLQNILRIEPDNLMALIRKRDLQIRLEKWSDALEIQHRLVKAN
                     LPESERRAESNLLLGCMYEVGRQLLERGHPDKARRYFRGAIKKDRTFLPAYIGIGEIL
                     VREGKTKNAVEILKKIYAKTRSIIILHRLEELFLELGEPDEIIRVYQEALQQDPHNAV
                     IQFYLGKLYYRLEMVDEAYDVLATLDGTQEQLVDYHKIMANLYLRKQHMDEAVGELKK
                     ALGFKKRVVVPYQCTQCHHELAEWSGRCRRCGRWNTYVALPWRDANQPDTDQDGQALR
                     IPNVPYQGIASPFETV"
     gene            5153..6184
                     /gene="bioB"
                     /locus_tag="PK224_18445"
     CDS             5153..6184
                     /gene="bioB"
                     /locus_tag="PK224_18445"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRB17783.1"
                     /translation="MTDFTILADKALRDEILTHEESLAVLQTPDSRLLELLQAAFTVR
                     ERYFGKTVRLQMLLNAKSGACQEDCGYCSQSAISKAPIERYGLLTQDQMVTGARRAAA
                     AKAQRYCIVISGRSPIDRDIADIASAVRSIKQEVPIQICCSLGLLNERQAKDLKAAGV
                     DRINHNLNTSEAYHPEICTTHTFQDRLATIRHARTAGLEICSGGIVGMGESDEDLIDL
                     ALALREVKPDSIPINTLHPASGTPMEHCAPLTPQRCLKALCLFRLLHPRTEIRIAGGR
                     EHNLRSLQPLALYPADSVFVNGYLTTPGQPAAEVWRMIEDLGFEIQVDAVPTKQGIPA
                     SEPVAGLHS"
     gene            complement(6175..8820)
                     /locus_tag="PK224_18450"
     CDS             complement(6175..8820)
                     /locus_tag="PK224_18450"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB17784.1"
                     /translation="MPLISPALFEVCARLGWTHLSTHPGRTLLTIIGVGLGVAATIAV
                     QTANVDVLRSFEDSVLAVAGPVTLEVSAGEAGMDERLIASVRGIAGVDSAKPVGELGV
                     RVAGRSQSFLILGLDLLDELNSMQGRLPATLETLWRPGEGDGMDGLLAPSGLLLGQGL
                     ASELGVGPKALLNLEAGGREVSVVITGVMGRRAEGPSAWDRLAVMDIAAFQRTFGLSG
                     RLDRIDVVTQASASVEQVAEAIARILPPAVTVRRPIQRSQQVESMVGAFQLNLSVLSM
                     VGLLVGMFLIYNTVSFTVAQRRREVGILRAIGMSEPMVVGLFLAEAGVFGVAGGVLGG
                     SLGLVLGNVLVGLVGRTIQDLYVPMADAPRTFGFPPGSVRLLTEAIVIGSGVSILGAL
                     GPSLDAGRTMVVGALAPGEYDVAQRVRAASLGVVGGGLLLFALGCALAGPVGGVPVFG
                     YLATFCLLAGLSCLVPILMQWVCRTRELGAASASPSLGGAIRHIAREQTTRGIGRNAV
                     TVSAFLVGVAIMVGVMVMIRSFRDTVEMWIDQTVMADFIVAPAGWPHVVRGGSSSQAL
                     PGSWRTRLAGATHVSAVDAYRDVRIELEGRPIALVSRDLALHAARSRYLFLEGDSAAI
                     LTRAAAGEGAILSEVVANHLHVIRGSQLSISTPVGEKSLPVLGVFYDYATDGGKIVID
                     RSLYQHWWNDDGVTVFPVYIDPGVDLEQARAALLETLADGSRGSLLPTVLSNGELRRE
                     ILRIFDRTFTLTYVLEAIAIIIAMLGIINTLVTSVVERRRELATLQALGSSRGQITAL
                     ILWEAGYLGLLGTAMGLVGGLALAWILIRVINRQSFGWTIQVSWPLGLMAEVAVLALV
                     ASLLAGFWPARWAGRQPLVEGLRYE"
     gene            9024..9254
                     /locus_tag="PK224_18455"
     CDS             9024..9254
                     /locus_tag="PK224_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB17785.1"
                     /translation="MRAKRSEGLTPRQKEILKLVSQGNTNRDIARRLDISVRTVEVHR
                     FNLMRRLKVRNVAQLLRQALQQGFLPKNFAFR"
     gene            complement(9305..10231)
                     /locus_tag="PK224_18460"
     CDS             complement(9305..10231)
                     /locus_tag="PK224_18460"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HRB17786.1"
                     /translation="MDLSVTIAGVKFPTCFMNASGALCVTREELITLGRSRAGAIVTK
                     SMTLEPRVGNPEPRYYGFPGGSINSMGLPNLGYRAYAEMIPELTRFGKPVIASIAGLC
                     EDDFLTMARVINQARPDLIEVNLSCPNIPGKPQIAYDPVDSERLLKRVRPLITVPMGV
                     KLPPYFDPAHHAVMADVIRRCGVDYLNLINSVGNGLVVDPKRETPVIKPKGGFGGLGG
                     SLIKPVALANVRAFWKLLEGRIPIIGTGGVVQGVDAFEHVLCGASAVQVGTALVEEGV
                     GVFERLERELAAELAMRGKQSLEECRGKLKEL"
     gene            complement(10376..11074)
                     /locus_tag="PK224_18465"
     CDS             complement(10376..11074)
                     /locus_tag="PK224_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB17787.1"
                     /translation="MQDPTNMLVRVQNLSKEYRRGQTLVSALQDVTLDVKAGEFCAFV
                     GPSGCGKSTLLNLIGGLDQPTAGTIAIENRPVTHASATEWTRLRRELIGIVFQAFHLI
                     PGLTVAENVALPLLLKGERGAAVAERVNEVLEAVRMDRRRSHRPGELSGGEQQRVAIA
                     RAIVHRPRLVLADEPTGNLDSKQGADIVSLFRTLPQRFGHSVLLVTHSDSAADAADYV
                     WHMRDGRLVNCVRQ"
     gene            11276..12088
                     /locus_tag="PK224_18470"
     CDS             11276..12088
                     /locus_tag="PK224_18470"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRB17788.1"
                     /translation="MSSHALFAGILRQATRLVLPSDCTTCEQPLTDDPTPFFCRQCWE
                     LIRPLQGPSCPRCHRPFASPFATTHSPTHECHDCRTRTPHYSQVWAAYAYCAPLQEAI
                     ALFKYRGKVVLADALGALLARALPADLNADLLMPIPLHPDRLRQREFNQSLLLADRVG
                     PVLRRPVSYRNLVRIVDTDPQTTLPRSARLQNLHQAFALRAPGEVTGKRVLLIDDVFT
                     TGTTANECAKVLLKAGAKDVAVLTLARSVDAGMVPETWLPPSAFDQPHALRV"
     gene            12091..>12343
                     /locus_tag="PK224_18475"
     CDS             12091..>12343
                     /locus_tag="PK224_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRB17789.1"
                     /translation="MPIYEYRCRQCGKRTTRLILSISAAPQQTCGHCQSPDLERLLSR
                     FASPKSEEARLEALSDPSHLSGFDENDPQSMARLMKKMGQ"
BASE COUNT         2717 a         4114 c         3326 g         2186 t
ORIGIN      
        1 gtcaaagcct acggcggaga gacgatcaag gcaggctcca tcattgtgcg ccagcgggga
       61 acgaagtttt tcccaggcct caacgtcggt ttgggccggg atcacaccct ctatgcctat
      121 gtgtccggtg tcgtgaagtt cgaaggtggc cgcggccgtc agaaggtcag cgtctatccc
      181 gtcacagcga aggcctgatc cctcctcagt ccataccttt gttcctacgg aacaggtgcc
      241 ttccgttaca gcatccccca tgagccggcg aatacgctat actgctgttt ctgaataacc
      301 aggccggctc ggggatcgct gtcatgtcta cctttgtcga tcaagtccaa atcctggtga
      361 aggccggtca cggcggcaac ggcgcctgca gttttcgacg ggaaaagttc gtccctcgcg
      421 gcggccctga cggcggtgac ggcggtgacg gcggcagcgt cgtggttgaa gccaccactc
      481 gcctcagcac cctgctggat cttcgttacc aaaaacatta cgaggccgaa aaaggcgaga
      541 ccggaggagg cagcaactgc catggacgcc ggggcgccga cgtgcgcatt cccgtcccgg
      601 tcggcaccat ggtgttcgac gaaaacaccc aggaactgct ggccgatctc acgaaagacg
      661 gagaaacctg cgtcattgcc aaaggcggtc agggaggccg aggcaacacc caatttgcca
      721 catcgaccaa ccgggtgccg acacaattcg agccaggcac tcccggcgaa gagcgattgc
      781 ttcgactcga tctcaagctc ctggccgatg tcggattggt cggctatccg aacgccggaa
      841 aatcgacact catcgccgcc gtgtccgccg ctcgcccgaa aattgccgat taccccttca
      901 ccaccctgac gcccaacctg ggcgtggtac ggtggaccgg cgagcagacc tttgtcatcg
      961 ccgatattcc aggattgatc gaaggggcac atgaaggcaa ggggctggga tttcagtttc
     1021 tgcgccacat cgagcgaacc agcctgctac tccatgtgat cgacatttca gaatgggcca
     1081 ccgaggaccc ggtcgcgagc ctggagatca tgcgccacga actgaccgct tacgatgaca
     1141 cattggcggc gcgccccttt gccgtcgtgg gcacaaaact cgacgtcaaa ggcgaggggg
     1201 aacggatcga acggctccgg aagtactgtc agcgacgcaa gattccattt tttgcgattt
     1261 cggcagccac gcgagaagga ctggacgagt gcatacgcta cataggccag caagtggaac
     1321 tgcttcggaa aaccccgtgc gagacgaact cctaagacaa gccaaacgcg tcgtcatcaa
     1381 gatcgggagc agcctgatcg cctcgcgcga aaccggcctg agcgccgagc ggatcgagcg
     1441 ccttgccggc gaaatcgccg aggtgcgggc acaggggcgc gaggtactgg tcgtctcatc
     1501 cggcgcggtc gtatccggca tcaaaaaact cgggttgcgc gaatacccga aaagcctgcc
     1561 gctcaaacag gctgctgctg ccgtcggcca aagcctcctc atgtgggcct acgaaaaggc
     1621 ctttgagcgc ctcacgctcc gcgtcgcaca ggtgcttctc acgcacgaag acctcgccga
     1681 tcggcgacgg tttctcaacg cccggcacac cctcaccacc ttgatcaagt tcggggtgat
     1741 tccgatcatc aacgaaaacg ataccgtcgc ggtcgaggaa atccgcgtgg gggataacga
     1801 tactctggcg gcgcaagtcg cccacctcgt cgatgcggat ttactgatca tcctctcgga
     1861 cgtcgacggg ctctttaccg tggacccccg caaggacccg acggccgcct tgattccact
     1921 gatccccgag attacggaag acatcgaaca gcgggccggc atgtcgtcca ccttcgaagg
     1981 cacaggcggc atggccacca aagtacgcgc tgccaagaag gtgggggaat acggcgtcgc
     2041 caccctgatt ctaaatggca cccagacaag actccttccg aacgtgcttt ccgggcaacc
     2101 cggcggcagc ctgtttctcg cccgggaacg acgcatgaac agtcgcaaac actggattgc
     2161 gtttaccctg cgcccccgcg gccatgtgca gctggaccag ggggccgtcg aggcactggt
     2221 acgaaacggc aaaagcctcc tggcatcggg catcctcgat attaccggcc atttcgacgc
     2281 cggcgatccg gtgacctgcg ccggtcctga cgggaaagaa tgtgctaagg gcctggtcaa
     2341 tttctcatca gagatcctga cgcgaatcaa ggggctcaag acggctgaca tccaaaaaat
     2401 ccccggtctt caggagtatg aagaggtcat tcaccgcgac aacctggtga tcctttagcc
     2461 agccgacgac cacccataca tggctcgggc gcgatgtcac caacacccga tctgaatacc
     2521 ggtccgccgc acacagacgt ttgcatggag cccgtacaat atgcgcctgg gactcttggg
     2581 gggcagtttt aatccgatcc accggtgcca cctctcgatt gcccgatctg cccgacagct
     2641 cctgcaactg gatcgcgtcc tgttcatccc gaccggtgat cccccgcaca aacaaccagg
     2701 cacgctggcc acggcatccc accggcaccg aatggtccaa ctcgccatcg aggacacccc
     2761 ggaattcgca ctcacagatc ttgaaatccg ccgatccggc aaatcctatt ccatcgacac
     2821 ggtccgcgcg atccagcagg aatacggccc cgacaccgtc ctctttttca ttatcggact
     2881 ggatgcgttt ctggatctgc cctcctggaa agaagcggac accctcctga agagctgtca
     2941 cttcgtcgtc atttcaagac cgtccacgag ctttcaggcg atggccgcaa tcccgctctt
     3001 ccgggacgtg ccgcaaacca ccctaaccgc gctggacgaa gtgcgacaag aacgcgcgga
     3061 cgtggccatc acaaacgggc aggctctgac attcctgcgg ttgcccccct gcgacgtgtc
     3121 cgcctcggag atcagggcac ggctgcgcag cggcgcctct ctggcaaatc tgttgcccac
     3181 ccctgtcgaa tcctatatac ttcgcgaggg gttatacagg gaggacagtg agcgtctctg
     3241 aatcaaaggc caaagctctc gcggtggcaa gcgccatcct ggacaaaaaa gccaccgatg
     3301 ttctcatcct gcatatcgcc acactgacct ccgtcgccga ctatctggtc atcggttccg
     3361 gggaatcgga gcgtcaagcg cgggcaatgg cggaccacgt gagcgacgtg ctcacggcgc
     3421 aaggtcacag gccgctcagc actgaaggtg catcgtcagc caagtgggtc gtcatggact
     3481 ttggtgacgt gatcgtccac attttccaaa aggatattcg cgaacactac gccctcgaac
     3541 gactgtgggg cgacgcggga caagttcgat tgccggaaaa gcgggccgtg aaagccgctc
     3601 ccccgacacg aaccgccaca cgtccggcgc gcacccggaa acgggtctag catgtttcgg
     3661 ctcctctcca cgatcttcct cgtcagcgtc ggcatctttc tctacagcta ttttcgcgaa
     3721 ctcaaccccg gtacgattac ggtgcgaacc agcccggacg cgctgttcga actgagcccc
     3781 gtctcattag tcctgttctc aatggccctg ggcgctacat tggtggctct catcgtcacg
     3841 atcaaagaaa cctcccatgt attcatgaat tggcggacca atcgactcgt ccgccgcaaa
     3901 gagaaggtcg atgccctgca tcgggacggg acgcacgcgt tcatgtccaa gcggacggcc
     3961 gatgccgtga acctgtttga acgcgcgctg gtgatcgatc cgaatcgaac ggattcgttg
     4021 ctctggctcg ggaatattta ccggtccgag agcaatttca cggaagccat ccgcctccac
     4081 cagcaggccc accgcattga agaacgcaat gtggaagtcc tgctcgaatt ggccaaggac
     4141 ctggaaggcg cccgacgata cgaggacgcg ctccaaacgc ttcagaacat tctccgcatt
     4201 gagccggata acttgatggc cttgatccgc aaacgtgatc tgcagatccg actggagaaa
     4261 tggagcgatg cgctcgagat ccaacaccgg ctggtcaagg ccaacctccc cgaaagcgaa
     4321 cggcgcgccg aatcgaatct gctcctcggc tgtatgtatg aagtcgggcg gcaactcctg
     4381 gagcgcggac acccggacaa ggcccgacgc tacttccggg gcgcgatcaa gaaagatcgg
     4441 acgttcctgc ctgcctacat cggtatcgga gagattctgg tccgtgaagg aaaaaccaag
     4501 aacgcggtcg aaattctcaa gaagatctat gccaagacac gcagcatcat catcctgcac
     4561 cggctggaag aactcttttt ggagctgggc gagccggacg agatcatccg tgtctaccag
     4621 gaggcgctcc agcaggaccc tcacaatgcc gtgatccagt tttatctcgg gaaactctac
     4681 tatcgactcg aaatggtcga tgaggcctat gacgtgctcg caacactcga tgggacacag
     4741 gaacaactcg tcgactacca taagatcatg gccaacctgt acctgcgtaa gcagcatatg
     4801 gacgaggccg taggagagct caagaaggcc ttgggcttca agaaacgggt cgtggtaccc
     4861 taccaatgca cccagtgcca ccatgaactg gccgaatggt caggccgttg ccggcgatgc
     4921 ggacgatgga acacctacgt cgcccttccc tggcgggacg cgaaccagcc cgataccgac
     4981 caggacggcc aggctttgcg catcccgaac gtcccttatc aaggcattgc atctccattt
     5041 gaaaccgtgt agggtttctc cgcccttttt tcttttcaca gacacggcct ccgcccacgg
     5101 gtctcccgat cgggaatcaa ggttcgatct gtcttttcga ggagtagcgt gcatgactga
     5161 cttcacgatc ctggccgaca aggccttgcg ggacgaaata ctcacccacg aggaatccct
     5221 cgccgtattg cagacaccgg actcccgact gctggaactg ttgcaggcgg ctttcaccgt
     5281 ccgtgaacgc tacttcggca agacggttcg cctgcaaatg ctcttgaacg ccaagagcgg
     5341 cgcttgccag gaagactgcg gctactgttc gcaatcggcc atctccaaag caccgatcga
     5401 acgctacgga ctgctcaccc aagaccagat ggtcaccggc gcccgccgcg cggcggctgc
     5461 aaaagcccag cgatattgta tcgtcatcag cggacgcagc ccgatcgatc gggacattgc
     5521 cgatatcgcc tccgccgtcc gctccatcaa gcaggaagtt ccgattcaga tttgttgctc
     5581 gctggggctc ctcaacgaac gccaggcgaa agacctgaaa gccgccgggg tggaccgaat
     5641 caatcacaac ctgaacacga gcgaagccta tcaccccgag atctgcacga cacacacctt
     5701 ccaggaccgg ctggccacca tccggcacgc gcggacggcc ggactcgaaa tctgttccgg
     5761 tggcatcgtc ggaatgggag agagcgatga agacctgatc gatctcgccc tggccctccg
     5821 tgaggtcaaa ccagactcga ttcccatcaa cacgctacac ccggcatccg gtactccaat
     5881 ggagcattgc gcccccctga cgccgcagcg gtgcctgaag gccctgtgcc tcttccggct
     5941 cctgcacccg cgaacggaga tccgtatcgc cgggggccgt gaacacaacc tgcgcagctt
     6001 gcaaccgctc gcgctctatc cggcggattc agtgttcgta aacggatacc tcaccacgcc
     6061 gggtcaaccg gcggcagagg tgtggcgaat gatcgaagac ctgggtttcg aaatccaggt
     6121 ggacgcagtg ccgaccaagc agggaattcc tgcgtcagag ccagtcgcgg gccttcattc
     6181 gtaacggagc ccctcaacca gcggttgccg gccggcccaa cgggccggcc agaacccggc
     6241 gaggagcgaa gcgaccagag ccagcaccgc cacttccgcc atcaacccca ggggccatga
     6301 cacctggatc gtccagccga acgactgccg gttgatgacc cggattaaaa tccaggcgag
     6361 cgcaagcccc ccaacgagtc ccatcgccgt tcccaacagg cccagatagc cggcttccca
     6421 gagaatcaga gccgtaatct gtccccggct gctgcccagc gcctgcaacg tggccaactc
     6481 ccgacgccgc tcgaccaccg acgtcaccaa ggtgttgatg atccccagca tggcgataat
     6541 gatcgcgatg gcctccaaca cataggtcaa tgtgaaggtc cggtcgaaaa ttcgaaggat
     6601 ctcccggcgc aattctccgt tgcttaacac cgtcggcaac agactcccgc gggagccatc
     6661 ggccaaggtt tcaagcaggg cggctctggc ctgctccaga tcgactcccg gatcgatata
     6721 cacaggaaac accgtcacgc cgtcatcgtt ccaccagtgc tgatacagag accggtcgat
     6781 gacgatcttc ccgccatcgg tcgcgtagtc gtaaaagacc cccagaaccg gcaacgattt
     6841 ttcccccacc ggcgtcgaaa tcgacaactg actgcccctg atcacatgca ggtgattggc
     6901 caccacctcc gagaggatag ccccttctcc tgctgcggca cgagtcagga ttgccgccga
     6961 gtcaccctcg agaaatagat atcggcttcg tgccgcatgg agcgccagat ctctggatac
     7021 caacgcgatc ggccgcccct ccagctcgat gcgtacatcg cggtacgcat cgaccgccga
     7081 cacatgcgtt gccccggcca gacgggttcg ccaggaacca gggagggcct ggctcgacga
     7141 cccaccgcga accacatgcg gccacccggc cggagccacg atgaaatcgg ccatgaccgt
     7201 ctggtcgatc cacatctcga ccgtgtcgcg aaaactccga atcatcacca tgaccccgac
     7261 cataatcgcg actccgacga gaaaggcaga gacggtcact gcattccggc cgattccccg
     7321 cgtcgtctgc tcccgcgcaa tgtgtcgaat ggcgcccccc aacgaaggcg aagcggaagc
     7381 ggcgcccaac tctcgggtac ggcaaaccca ctgcatgagg atcggaacca ggcaggagag
     7441 accggcaagc agacagaacg tcgccagata cccgaagacc ggaaccccgc cgactggtcc
     7501 ggctagcgcg catcccagcg cgaagagcag caggcctcca cccaccaccc ccagtgaggc
     7561 agcacgaact cgctgggcaa catcatattc cccaggagca agggccccga ccaccatggt
     7621 acgtccggca tccaagctgg gccccagcgc tcccaggatt gacaccccgc taccgatcac
     7681 aatcgcttcg gttaacagcc ggacagatcc cggcggaaag ccgaaggttc ttggcgcatc
     7741 agccatcggg acatagaggt cctggatggt ccgaccgacc aacccgacga gtacattccc
     7801 cagaaccagg cccagactac cccccaggac ccctccggcc acaccgaaca ctcccgcctc
     7861 cgcgaggaac agacccacga ccataggctc cgacatcccg atcgcccgca ggattcccac
     7921 ctctcgccgg cgttgcgcca cagtgaagga cacggtgttg tagatcagga acatcccgac
     7981 tagtagcccc accatactca gcacggagag attcaattgg aatgcgccca ccatcgactc
     8041 gacttgctgg ctgcgttgga tcggacggcg cactgtgaca gccggaggca gaatccttgc
     8101 gatcgcctcg gccacctgtt ccaccgacgc ggaggcctgc gtcaccacgt cgattcgatc
     8161 caaccggccg ctcaaaccga aggtccgttg aaaggccgca atatccatca cggccagacg
     8221 gtcccatgcc gacggcccct cggcccgacg acccatcaca cccgtgatca caactgacac
     8281 ttcccgccca ccggcttcaa ggttcagcag cgcctttggg ccgactccca actcagaagc
     8341 cagcccctgt ccaagcagga gtccgcttgg agccaggagg ccgtccattc catccccctc
     8401 acctggccgc cagagagtct ccagcgtcgc agggaggcgc ccttgcatgg aattcaactc
     8461 atccagcaga tccagtccaa ggatgaggaa cgattgagaa cggccggcca ctcgaacccc
     8521 gagctctccc accggtttag ctgaatccac accggcgata cctctcacgg aagcaatcag
     8581 gcgctcgtcc atgcccgctt ctccggccga tacctctagt gtgaccggac ccgccacggc
     8641 taagaccgag tcctcgaacg atcggaggac gtccacgttg gcagtctgga ccgcaatcgt
     8701 cgccgcaacc cccaacccga cgccgatgat cgtaaggagc gtgcgaccgg gatgagtgga
     8761 caaatgtgtc catccaagac gggcacagac ctcgaatagg gcaggggaaa tcagcggcat
     8821 cggcggacct accggaacaa cactctagta attgttttta cagatttacc cagatatgct
     8881 aagattacgt tcagtagaat ttggggtaca atatctatat ggcactacgc aaacgacctc
     8941 aagccagaaa gccagcgaag atcaccaagg ccgcttcacc gaccaagaag agaaaacctg
     9001 caagggtcgt aaagccggcg cccgtgcgcg cgaaacgatc cgagggactg acgcccaggc
     9061 agaaagaaat tctgaaactg gtgtcccaag gaaataccaa ccgcgacatc gcccgccgcc
     9121 tggatatcag cgtgcgcacc gttgaggtgc atcgcttcaa tttgatgcgg cgcttgaaag
     9181 tccgcaacgt cgcgcagctg cttcgtcagg cgttgcaaca ggggttcctt ccgaagaact
     9241 tcgccttccg ataacactct atgcgccggt ttcatatacg gaaccggcgc acctttttac
     9301 agctttacag ctctttcagt tttccccgac attcctcaag cgactgcttg ccccgcatgg
     9361 ccaactcagc cgccagctcg cgctccaatc gctcaaacac ccccacacct tcttcgacca
     9421 gcgccgtccc cacctggacg gccgacgcac cgcacagcac atgctcaaag gcatcgactc
     9481 cctgcacgac tccaccggtc ccgatgatgg ggatacgtcc ctccaacaac ttccagaacg
     9541 ctcgcacatt ggccagcgcg accggcttaa tcaacgatcc ccccaaccct ccgaatccgc
     9601 ccttgggctt gatgaccggc gtttcgcgct tcgggtccac aaccagaccg ttccccacag
     9661 agttgatcaa attgaggtaa tcgaccccgc agcgtcgaat cacgtccgcc atcaccgcgt
     9721 ggtgggccgg atcgaaataa ggcggcagtt ttacgcccat tggaacggtg atgagcggtc
     9781 gcacccgttt gagcaaccgt tccgaatcga ccggatcgta ggcgatctgc ggcttaccgg
     9841 gaatattcgg acaggagaga ttgacctcga tgagatcggg ccgcgcctga ttgatcacac
     9901 gcgccatggt gagaaagtcg tcctcgcaca ggccggcaat gctggcgatc acgggtttcc
     9961 cgaaccgggt gagttcgggg atcatctccg cgtaggctcg atacccgaga ttcggcagcc
    10021 ccatggaatt gatggacccc cccggaaatc cgtaataccg cggttcagga ttaccgaccc
    10081 gtggctccag ggtcatcgat ttagtcacga tggcaccggc ccgggaacgc cccagcgtaa
    10141 tgagttcctc ccgcgtcaca cagagcgctc cggaggcatt catgaaacag gtcgggaatt
    10201 ttactcccgc aatagtgacg ctcaaatcca tgctgatcct ttctcccgac aggacatgga
    10261 acaaccccgt cggcgtgcct cccccatcct ctttgaaatt cgcgaggcgt tattcataac
    10321 gcctctctcc gagcgacgag ccgacttcac gaacatagta cacaaccgtt cgaggctatt
    10381 gccggacaca attcaccaat cgtccgtccc gcatatgcca cacataatcg gctgcgtcgg
    10441 ccgcactatc gctgtgcgtc accagcaaca ccgagtgacc aaaccgttgc ggtaacgtac
    10501 ggaacaggct gacgatgtcc gccccttgct tggaatcgag attcccggtc ggttcatccg
    10561 ccagtacaag tcgtggccga tgcacgatcg cccgggcaat cgcgacacgc tgctgttccc
    10621 ctcccgacaa ctcgccgggc cggtgcgaac gtcgcctgtc catccgcacc gcttccaaga
    10681 cttcgttgac ccgctcggcc accgcagccc cccgctcccc cttcagcagc aacggcaagg
    10741 cgacattctc cgcaactgtc aggccgggaa taaggtggaa ggcctgaaag acaattccga
    10801 tcaactcgcg tcgcaagcgg gtccattcgg tggcgctcgc atgggtcaca ggccggttct
    10861 caatggcgat ggttcccgcc gtgggctgat ccaaccctcc gatcagattc agcaacgtgc
    10921 tcttcccgca cccgctcggc ccgacgaagg cacagaactc tcccgccttc acgtcgaggg
    10981 tcacgtcctg caacgccgac acaagcgtct gcccgcggcg atattctttc gaaagattct
    11041 gcacccgtac cagcatgttg gtgggatctt gcatgatggg aatcgatagc cctgacggag
    11101 tgcggaagca tccggcagat gcatgcaacc ggcacattgc cgagcctctg gaatcgctcc
    11161 gatgcaggcg agtggtccta taccacaccc ggtttgtacc ccacaacctt gacggctgag
    11221 gggtcttccg tacacaatcc caacgcgcgt ccgtcacgcc tcttggacct agccaatgag
    11281 ctcacacgca ctgttcgcag gcatcctcag gcaagccaca cgactcgtac tcccgtcgga
    11341 ctgcacgacc tgtgaacagc ccctcacgga cgatcccacg ccgttcttct gccgccaatg
    11401 ctgggaactg attcgaccgc tacaagggcc ctcctgcccg cgatgccacc gccctttcgc
    11461 ctcccctttc gcgacaaccc atagcccgac tcacgaatgc catgactgcc gtacccgcac
    11521 accgcactat tcgcaggtgt gggcggccta tgcctactgc gcaccgctcc aggaggccat
    11581 tgccctcttc aaatatcgcg gaaaggtggt gctggcggat gcgctgggtg cgttgctcgc
    11641 ccgggctctt cccgcagatc tgaatgcgga tctcctcatg ccgatcccgc tccaccccga
    11701 ccgccttcgg cagcgagaat ttaatcaatc actcttgctc gcagaccgcg tcggacctgt
    11761 actgaggaga cccgtatcct atcgaaacct ggttcgcatc gtcgataccg acccccagac
    11821 cacgctcccc cgctccgcac gcctgcagaa ccttcaccag gcgtttgccc tgcgcgcccc
    11881 aggggaagtg accggcaagc gcgtcctcct catcgacgat gtcttcacca cgggcaccac
    11941 agccaacgaa tgcgccaaag tcctgctcaa agccggtgcc aaagacgttg cagtgctcac
    12001 gctcgcgcgt tccgtggatg ccggcatggt gccggagacc tggctcccgc catcggcctt
    12061 cgatcaaccg cacgcattga gggtctaact atgccgatct acgaatatcg ctgtcgccaa
    12121 tgtgggaaac gaactacccg cctgatcctc agcatcagcg ctgcgcccca acagacatgc
    12181 ggccactgtc agagcccgga tctggaacga ctgctctccc ggtttgcctc tcccaaatcg
    12241 gaagaagcac gccttgaggc cctgtccgat ccaagccatc tgagcggatt tgacgaaaac
    12301 gacccgcaga gtatggcccg gctcatgaaa aaaatggggc agg
//
LOCUS       DAORRQ010000089        12342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_248_length_12342_cov_198.987141, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000089 DAORRQ010000000
VERSION     DAORRQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12342
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_248_length_12342_cov_198.987141"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..94
                     /locus_tag="PK224_18480"
     CDS             <1..94
                     /locus_tag="PK224_18480"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17790.1"
                     /translation="HQPQRALSPGQSAVFYDGDRVLGGGIIQRS"
     gene            344..883
                     /gene="atpH"
                     /locus_tag="PK224_18485"
     CDS             344..883
                     /gene="atpH"
                     /locus_tag="PK224_18485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRB17791.1"
                     /translation="MIKTAVARRYAKALFELLDTPSIEPARSALNGLGEAFTQSAALR
                     HAIASPVFPEETKLGALIELATRLGCPPVGQRFLELLVKKNRVSFLPDIAEAFAKLVD
                     QSKGTQQVLVSSANALPAAEQDRITTRLRDLLQRDVDVTFHTEPEHVAGLHIRLGSTV
                     VDSTVRGRLSAMQRVLTKE"
     gene            886..2403
                     /gene="atpA"
                     /locus_tag="PK224_18490"
     CDS             886..2403
                     /gene="atpA"
                     /locus_tag="PK224_18490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRB17792.1"
                     /translation="MQIKAEEISSIIKEKIKGFDQQVDVSQTGSVIQVGDGIAKIYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGDDVGIKEGDPVKRTGRIAEIPVGEA
                     LIGRVVNAIGQPIDGKGPINSQHSSRIEVVAPGVNTRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGLGVFCIYVAVGQKRSTVARVVKTLEENHAM
                     EYTMVVAATASDPAPMQFLAPFSGAAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFSQFGSELDKATQMQLARGVRMVELLKQGQYKPMPVADQVLSIYAGVNGFLDDVPV
                     DKVLQFEADLLHYVAQNHQDMRKDIATVGKIDDKVGAKLKEIITTFKQKMGYGAK"
     gene            2468..3355
                     /gene="atpG"
                     /locus_tag="PK224_18495"
     CDS             2468..3355
                     /gene="atpG"
                     /locus_tag="PK224_18495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRB17793.1"
                     /translation="MPSLQSLRRKIAAFKNTQKITKAMKMVAAAKLKRSQDRILAARP
                     YAHKMRGVLSNLSQRVNRTSHPLLQKREGKKIEILVVTSDRGLCGGFNGNIVRKSSEF
                     VRQCEAQGLSVNLSLIGRKGRDYFRRRAWPIRQEWTGIFDKLSFEHAIDIGGDLTENF
                     VKGTFDELYVVYNEFKSAIQQRVIVEKLFPIDAAAEFGAAPTATEQSAVGGSYLYEPD
                     EGELLNVLVPKHFQIQAYRILLESAAAEHGARMAAMDGATRNAGQLIKKVTLYYNKTR
                     QAAITKELMDIVGGAEALK"
     gene            3424..4866
                     /gene="atpD"
                     /locus_tag="PK224_18500"
     CDS             3424..4866
                     /gene="atpD"
                     /locus_tag="PK224_18500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRB17794.1"
                     /translation="MSTGKVIQVIGPVVDVEFPPGQLPNIYNALKVTQEENKAAGKPA
                     VRITLEVASHLGENRVRGIAMSTTDGLTRGMDVQDTGAPISVPVGRETLGRLINVLGE
                     PVDEKGPIKAKKTYPIHRPAPRLEDQDTKTEVLETGIKVVDLLEPYSKGGKVGLFGGA
                     GVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQESKVIDPDDHTKSKA
                     ALVYGQMNEPPGARLRVALTGLAVAEFFRDEENQDVLLFVDNIFRFTQAGSEVSALLG
                     RMPSAVGYQPNLSTEMGALQERITSTKKGSITSVQAIYVPADDLTDPAPATAFAHLDA
                     TTVLSRQLAELGIYPAVDPLDSTSRILDPQVIGEEHYKVARGVQSVLQRYKDLQDIIA
                     ILGMDELSEDDKMAVARARKIQRFLSQPFHVAEAFTGAPGKYVKLKDTVRSFKEILEG
                     KYDHLPEQAFYMVGPIEEAVAKAEKMGVKV"
     gene            4992..5414
                     /locus_tag="PK224_18505"
     CDS             4992..5414
                     /locus_tag="PK224_18505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRB17795.1"
                     /translation="MAGKILLEVVTPEKLLLSQEVDEVIAPGSEGEFGVLPGHCHFLS
                     SLRIGELRYKTQDAWHYMSILWGYAEVTPSKVTVMAEIAEKAEDIDVGRAQQAVEKAE
                     QRLQAGGLPSEVKEAQISLEKARLRKKIADRAKKSGHA"
     gene            5726..6385
                     /locus_tag="PK224_18510"
     CDS             5726..6385
                     /locus_tag="PK224_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17796.1"
                     /translation="MDNARPPRSVFSLEEINLLMPTVRKSFQEATSREWIVFMLVGQE
                     GTGRETTSGGMFVEEGKLHLVIANHRLALAENSEELARVRANPLHSVQGSGGALVFDL
                     PRFVIGTKANWSGGHRASASELILDHAAFLSSLQHRGSAAAPVPVSGSHAPVSAPEAD
                     APGPGSFHSGQVDSEQIILQLREEIEVLKQQLADKDAEIDRLKPREIRTTPAPVIRTP
                     P"
     gene            complement(6504..7100)
                     /locus_tag="PK224_18515"
     CDS             complement(6504..7100)
                     /locus_tag="PK224_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB17797.1"
                     /translation="MTSSQKLLRVTTLLGFALVCATVLWPSHALPENAPVTLPIETVA
                     NYLHAVIEADRDVYTRHVVERLQTKGVVVASENWEQKNTLPLPAQFLMESGRYIAKKG
                     LGIQYRLISLWPINKRNVAANELEKAGLGTILTQPNRPHTGFMKIGETRYFQAVYADL
                     AATQACLGCHNAHPDSPKRDFKLNDVMGAIVITIPVGQ"
     gene            complement(7904..8674)
                     /locus_tag="PK224_18520"
     CDS             complement(7904..8674)
                     /locus_tag="PK224_18520"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17798.1"
                     /translation="MTFCLGMKVEEGLVGIADTRVTTGAECITAGKVSIHQHGRHSMF
                     LMTSGLRSVRDKAVTYFDEAISESDQTFDKLFKAVNVFAAQIRRVAEEDKSALYNAGL
                     TFNLHALVGGQLENDLTHKLYLIYPQGNWVEVSEGTPYCIIGESGYGKPLLDRVLRYN
                     SSMDLALKVGFLAFDATRTSSTSVEYPLDVVLYRHDTFDILEHRFQKEDLAEISSWWQ
                     SRIADSVEKLPASWVDRLLEALPKGTRSSPQDHGNSAS"
     gene            complement(8671..9651)
                     /locus_tag="PK224_18525"
     CDS             complement(8671..9651)
                     /locus_tag="PK224_18525"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRB17799.1"
                     /translation="MLSRVASSIYWVNRYIERAENYARFIEVNLNLSLDLPRGTVEQW
                     EPLVATTGDHEMFVARYGKATKETVLHFLVADAENQNSILSCLLGARENARSIKEIIP
                     SEMWEQVNRFYLMVRDAVTRGMSHHDLHAFLADVKAASHLLWGITDATMSHGEGWHFA
                     RLGRLLERADKTSRILDVKYFILLPTADEVGTPFDIIQWSDLLKSASALEMYYKRYGR
                     IAPDDVAAFLILEPTFPRAIRYCLIKAEDSLHAISGSERGSYQNLAEKRLGRLRAELD
                     FVDIEDCVTRGLHEFVDHFQARLNQVGEAISETFFVPRRVHYTESVTEEQ"
     gene            complement(9725..11149)
                     /locus_tag="PK224_18530"
     CDS             complement(9725..11149)
                     /locus_tag="PK224_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRB17800.1"
                     /translation="MKFSTYDPGEFYDELFEGIGRPRRGSALLLRKFASLPDGELRKR
                     QQAAERVILNMGMTFGVYGSDEGHEQIFPFDIVPRIVEAPDWTLIDTGLRQRLRALNL
                     FIDDVYHEQKILKDNVIPSELIYSSKGFLKACWGLNPPRGIWCHIAGIDLVRISDGRY
                     YVLEDNMRCPSGVAYVLEARQVMKRTFPELFEAYRVRPVDEYPSRLLETLRSLSDLPD
                     PTIVILTPGSYNSAYYEHSLLAQKMGVELVEASDLVVIDGSVHMRTTKGSQRVDVIYR
                     RINDEYLDPLVFRPDSLLGVPGVMEAYKNGRVAIANAPGTGVADDKAIYAYVPKIINY
                     YLAEEPILPNVPTYVCWERRDRDYVLEHLDQLVVKATNEAGGYGMMIGPQASQQERED
                     CARRIQADPRNYIAQPTLALSRSPTLVEDHIEGRHVDLRPYVLQGKGFYVLPGGMTRV
                     ALRKGSLVVNSSQGGGNKDTWVLS"
     gene            complement(11237..11815)
                     /locus_tag="PK224_18535"
     CDS             complement(11237..11815)
                     /locus_tag="PK224_18535"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB17801.1"
                     /translation="MGGKLAFVATCLILASGLAKTGLAAELPGIPPEVVADYLHAVIE
                     ADRTFYTIHVVERMQKQGGTPASETWRKDKTTLPLPAQFLREASELATLTGTRVRYRL
                     MSLWPINPQNAPASNSERNSLEAVRLHPERSVSGTVTIGSQSYFQAVYADRAVTQACI
                     ACHNAHPQSPKHDFKIDDVMGGMVIEIPLSKE"
     gene            11946..12302
                     /locus_tag="PK224_18540"
     CDS             11946..12302
                     /locus_tag="PK224_18540"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRB17802.1"
                     /translation="MQTEFVISAGEQPKRLDVFLVHREPKLSRAALQRMITAGWVRVN
                     SLIAKSSQRIKAGDVIVFDAPQAAPLRIDGETKQLEILFEDHACLVLNKPVGIVVHPS
                     PGHWSNTLLNALLAVC"
BASE COUNT         2624 a         3461 c         3635 g         2622 t
ORIGIN      
        1 tcatcagccg cagcgcgcct tgagcccagg tcagtccgcc gtgttttatg atggcgaccg
       61 cgtactcggt ggcggcatca ttcagcggtc atagccagtg ccactctcca attttttctc
      121 cagtaaatca attttgcgaa gcgggggctc agtggaggcc gtgcgcaagc ggcactggcg
      181 cgggtctcat gcttgttgcg atgtctcatc tgcggtctcc ccacgcatca cttcttgaca
      241 gtacccgctt ttgaatgcta acttggcgcg ctttttgtcg tgcccaccgc acgcaagctt
      301 cccgtttgat caaaacacgg ttaaccgtag cggatagtcc attgtgatta agacagccgt
      361 cgcacgtcga tacgcaaaag ctctgttcga acttcttgat accccaagca tcgagcccgc
      421 ccgctcggcg ttgaacgggc tgggagaggc cttcacgcag tccgctgcgt tacgccatgc
      481 catcgcttct cctgtgttcc cggaagaaac caaattgggt gcgctgattg aattggccac
      541 tcgactcgga tgcccgccgg tcggccagag atttctcgaa ctcttggtca aaaagaatcg
      601 ggtcagtttc ctgccggaca tcgccgaggc cttcgccaag ctcgtggatc aatccaaggg
      661 gacgcagcaa gtgctcgtgt catccgcgaa tgccttgccc gcggccgagc aggatcggat
      721 caccacccga ttgcgcgatc ttttacagcg cgacgtcgat gtcaccttcc atacagaacc
      781 tgaacatgtc gccggcctcc acatccgcct gggcagcacc gtcgtagaca gcacggtccg
      841 gggccgactg agcgccatgc agcgtgtgtt gaccaaggag tagccatgca gatcaaggca
      901 gaagagatca gctccatcat taaagagaag atcaagggct tcgatcaaca ggtcgatgtc
      961 agccagaccg gttccgtcat ccaggtcgga gacggcatcg cgaaaatcta cggattagac
     1021 ggcgcgatgg ccggagaaat gctcgagttc ccggggggcc tgtacggcat cgcgttgaac
     1081 ctcgaagaag acaatgtcgg cgccgtgttg atgggtgatg atgtcgggat caaggaaggc
     1141 gatccggtca agcgcactgg gcgtattgcg gaaatcccgg ttggtgaagc gttgatcgga
     1201 cgggtggtga acgcgatcgg tcagccgatc gacggcaaag gcccgatcaa ttcccaacat
     1261 tcttcccgta tcgaagtcgt ggcacccggt gtgaacacga gacagtcggt gcgtgagccg
     1321 ctgcaaacgg gcatcaaggc catcgacgcc atgatcccca tcggtcgtgg tcagcgtgag
     1381 ttgatcatcg gagaccggca gaccggaaaa acagctatcg ccgtcgatac catcatcaac
     1441 cagaagggcc tcggtgtatt ctgtatctac gtcgccgtcg gtcagaagcg ttctacggtt
     1501 gcgcgggtgg tcaagaccct cgaagaaaat cacgccatgg aatacaccat ggtcgtggcg
     1561 gcgacggcca gcgatccggc gccgatgcag ttcctggccc ccttctccgg cgccgcgatc
     1621 ggtgaatatt tccgcgacaa cggcaagcat gcgttgattg tgtacgacga tttgtccaag
     1681 cacgcggtgg cctatcgtca gctgtccttg ctgctgcgcc gtccgccggg ccgtgaagcc
     1741 tatccgggcg acgtgttcta tctgcactcc cgcttgctgg agcgcgcggc gaagttgagc
     1801 gataagttgg gcggaggcag cctgacggcc cttccgatca tcgagacgca agccggcgac
     1861 gtgtcggcct acattccgac caacgtcatt tctattaccg atggacagat ttacctgggc
     1921 agcgacttgt tctattccgg tattcgtccc gcgatcaacg tcggattgtc ggtgtcacgt
     1981 gtcggcggat ccgcgcagat caaaactatg aagcaggtcg caggtacgct ccggttagat
     2041 ctcgcgcaat atcgtgaaat ggccgccttc tcccagttcg ggagcgaact cgacaaggcg
     2101 acccagatgc agctcgcccg tggcgtacgt atggtggaat tgctcaaaca agggcaatac
     2161 aagccgatgc cggttgccga tcaagtcttg tcgatttacg cgggcgtcaa cggattcctg
     2221 gacgatgtcc ccgtagacaa ggtgctgcaa ttcgaggcgg acctgctcca ttacgtggcg
     2281 cagaatcacc aggacatgcg gaaagatatc gccacggtcg gaaagatcga tgacaaggtc
     2341 ggcgccaagc tgaaagaaat catcacgacc tttaagcaga aaatgggcta cggagcaaaa
     2401 tagcaatcag ccttcgcctt cagcatggag ctgaggtctg agcgctgact gctgagagcg
     2461 tacaagaatg ccgagcttac aatctctccg gcggaaaatc gccgccttca agaatacgca
     2521 gaagattacc aaggccatga aaatggtcgc ggccgcgaag ctcaagcgtt cgcaggatcg
     2581 cattttggct gcgaggcctt acgcccataa aatgcgcggg gtgctcagca atctcagtca
     2641 gcgcgtcaac cgtacctccc acccgctgct tcaaaaacgc gaggggaaga aaatcgaaat
     2701 tctcgttgtc acgagcgacc gcggcttgtg cggcggattt aacggcaaca tcgtgcggaa
     2761 gagctcagag ttcgtccgac agtgcgaagc gcaggggctc tcggtcaatc tcagcctgat
     2821 cggccgtaag gggcgtgact atttccgccg tcgtgcctgg ccgatccgac aggagtggac
     2881 cggaatcttc gacaagctga gcttcgaaca tgccatcgac atcggcggcg acctgacgga
     2941 aaattttgtg aagggcacgt tcgacgaact ctacgtcgtc tacaacgagt tcaagtctgc
     3001 gatccagcag cgggtgatcg ttgagaaatt gttccccatc gatgcggccg ccgaattcgg
     3061 agcggcgccg actgcgaccg agcagtccgc agtcggcggt agttacttgt atgagccgga
     3121 tgagggcgag cttctgaatg tcttggtccc gaagcatttt cagattcaag cctatcgcat
     3181 ccttctggag tcggcggcgg ccgaacacgg ggcacgcatg gcagccatgg acggtgcgac
     3241 tcgcaatgca gggcaactca tcaaaaaagt caccttgtat tacaacaaga ctcgccaggc
     3301 ggctatcacc aaagaactca tggacatcgt gggcggcgca gaagccctga aataaaatgt
     3361 ggaatgagga atgctgcatc acggtaatgt gccgggtgca tcattgacga caagaggagc
     3421 cttatgagca caggaaaagt cattcaagtc atcggccccg tggtcgacgt ggaatttccc
     3481 cccggtcaat tgccgaacat ctacaatgcg ctcaaggtga cgcaggaaga gaacaaggca
     3541 gccgggaagc cggccgtgcg gatcacgctt gaagtcgcat cgcatctcgg tgagaaccgc
     3601 gtccgcggca tcgcgatgtc gacgacggac ggtctgactc gcggcatgga tgtgcaggat
     3661 acgggcgccc cgatttctgt tcccgtcggt cgtgagaccc tgggacggct gatcaacgtg
     3721 ttgggtgagc cggtcgatga aaagggtccg atcaaggcga agaaaactta ccccattcac
     3781 cggcctgcgc cgcgattgga agatcaggac acgaagaccg aagtgctcga gaccggtatc
     3841 aaggtggtcg atctgttgga gccgtacagc aagggtggca aggtcgggct cttcggcgga
     3901 gccggagtcg gcaagaccgt catcatcatg gagctcatca ataacatcgc gctccaccac
     3961 ggtggattct cggtgttcgc cggcgtcggt gaacgtaccc gtgagggcaa cgacctttgg
     4021 cacgaaatgc aggagtccaa ggtcatcgat cccgacgacc atacgaagtc caaggcggca
     4081 ttggtctacg gtcagatgaa cgagccaccc ggagcccgtc tacgggtcgc gctgactgga
     4141 ctcgctgttg ccgaattttt ccgcgacgaa gaaaatcagg acgtgttgtt gttcgtcgac
     4201 aatatcttcc ggttcacgca ggcgggatcg gaagtgtccg cgttgcttgg ccgtatgcca
     4261 tcggcggtcg gttatcagcc gaacctgtcc accgaaatgg gtgcgctaca ggaacggatt
     4321 acgtctacca agaagggctc catcacttca gtgcaggcga tttacgtgcc ggccgacgac
     4381 ttgaccgacc cggctccggc gacggccttt gcgcacttgg atgcgacgac cgtgttgtcc
     4441 cggcagttgg ctgagttggg tatttacccg gcggtcgatc cactcgattc cacgtcacgt
     4501 attctcgatc cgcaagtcat cggagaagag cattacaagg tggcccgcgg cgttcagtcc
     4561 gtgttgcagc ggtataaaga tttgcaggac atcattgcca ttctcggtat ggacgaattg
     4621 tctgaagacg acaagatggc cgtggcgcgc gcgcgcaaaa ttcagcgctt cctttcccag
     4681 ccgttccatg tggccgaggc gttcaccggc gcgccgggca agtacgtgaa gctcaaggat
     4741 acggtccgga gcttcaagga aatcctcgaa gggaagtacg accacctgcc ggagcaggcg
     4801 ttctacatgg tcggtccgat cgaggaagcc gtcgcgaagg cggaaaagat gggagtcaag
     4861 gtttaggagc gcggaaaccg cgcgtcctcg gtgctcgtcg ccacgcgaag ttttggaaga
     4921 cctcgttgaa cgcgcgcaga agaagacgca ttgattcccg ctcatgcggg acaagggagt
     4981 cgtaagagaa gatggccggg aagattctat tagaagtcgt gacgccggag aagttgctcc
     5041 tgagccagga ggtcgatgaa gtcatcgctc cgggtagcga gggagaattc ggcgtcctgc
     5101 ctggacactg ccattttctc tccagcctgc gcatcggaga gctgcggtac aagactcagg
     5161 acgcgtggca ctacatgtcg atcctgtggg gctacgccga ggtcacacca tccaaagtga
     5221 ctgtgatggc ggagatcgcc gagaaggccg aagacatcga tgtcggtcgt gcgcaacagg
     5281 ccgtcgaaaa ggccgaacag agactgcagg ccggtgggct cccttccgaa gtgaaggaag
     5341 ctcagatcag cctcgaaaag gcccgtctcc gaaaaaagat cgcggaccgt gccaaaaaat
     5401 ccgggcacgc ctaacctctc ttcccgatac ttcacgtagt ctgccgattg cacctccctg
     5461 atccgtcgtc gggggacctc cggtgttacg aaccacgtca ttcaattctt acttccttgg
     5521 cctcacgatc ctcctctgtc tcggttgtac cgcgcccacg ctaacgagtc gtgtcattga
     5581 acaggactcc tcctggtttg tgcggctcga ctcccttctc gaagccggca gtgcatcccc
     5641 acgatatgat catccgagtg tttggaggga tgaagaactc tttgccatcc tcagccgatt
     5701 gttgcttcag gaacgagtcg gtcttatgga taacgcgaga ccgccgcgtt cggtattctc
     5761 gttggaagag atcaacctcc ttatgcccac tgtgcgtaag tcctttcaag aagcaacatc
     5821 ccgtgaatgg atcgtcttta tgttggttgg ccaagagggg acgggtcgtg agaccacgtc
     5881 cggcggcatg tttgttgagg agggcaagct ccatctggtg atcgccaatc accgtctcgc
     5941 gctcgcggag aattcggaag agcttgcgcg tgtgcgggcg aatcctctcc attccgttca
     6001 aggcagcggc ggcgccttgg tctttgactt acctcgattt gtgatcggga caaaagccaa
     6061 ttggtcaggt gggcatcgag cgtccgccag cgaattgatc ctcgatcatg ctgcatttct
     6121 ttcctctctc cagcacaggg gatcagcggc ggctcccgtt cctgtcagtg gatcgcacgc
     6181 gccagtctcg gcaccagaag ctgatgcacc agggccggga tcattccact ccggccaggt
     6241 ggattcagaa cagatcattc ttcaacttcg ggaagagatc gaagtactga agcagcaact
     6301 cgcagacaag gacgcagaga ttgatcgatt gaagccacgc gagatccgca ccacaccggc
     6361 tcccgtgatc cgtactcctc cctgaaacag actcgacaag gttttaggtg aaggaccgga
     6421 cctgttccca ccggcaggat cgtggtccgt cctgccggta ttgtcgtggc gcctgagtga
     6481 cagagtgccg aacatccgcg gcattattgc ccgacgggga tcgtaatcac gattgcaccc
     6541 atcacatcat tcagcttgaa atcacgtttg gggctgtcgg gatgcgcgtt gtggcaaccg
     6601 aggcaggctt gggtggccgc gagatcggca tacaccgctt gaaaatagcg cgtctccccg
     6661 atcttcataa agccggtgtg gggtcgattc ggttgcgtca agatggttcc gagcccggcc
     6721 ttctcaagct cgttcgccgc gacattgcgc ttgttgatcg gccacaagct gatcagccgg
     6781 tactgaattc ctaacccttt cttggcgatg taccgtcccg actccatcaa aaattgagcc
     6841 ggtaggggca gcgtattctt ctgctcccag ttctccgatg ccacgaccac accctttgtt
     6901 tgcaagcgct cgacgacatg tctggtatac acatcccggt ctgcttcaat cacggcgtgt
     6961 agatagttag ccaccgtttc gatcggcagg gtgaccggtg cattctcagg cagtgcgtga
     7021 ctgggccaca acacagtcgc gcagaccaat gcgaatccca gcaaggtcgt gacccgcagc
     7081 aatttttgag aggatgtcat accaacctcc ttgttgcgca cacgggtgat ccggtctggc
     7141 cggcgcacca cgtgagcaga tgaattgcac catacggtct ggccggtgag tgcttggtga
     7201 agtggagagg acgaacggga tcgatcgccg ggtgaatgag gataatgagt tgagaggtat
     7261 cgacgcggag gacggggcgg agcgatgagc cgggatgccg gccctatcga tggcctcctg
     7321 aaaaaacgac cggtcgtggg aaggtaccga aggcatcgcc ccagctcctt gcccgtacga
     7381 accaacgtgc ccaccagatc atacggtcac tcatcatatt acgcttcgat ctatacgctg
     7441 tctaatcatc ttatgggagg gcacgacacc atcggtggcc actaggggaa atacgtccat
     7501 ttctttaccc gtcatcttgt cgcatatatc gcaatggctc gggccatgat gtgaagtacg
     7561 accttcggtg cgcacgagat cagggcaacg tgggcgagcg gcgaggtcgc gtcaaaccgt
     7621 acgccattcg ctggtcagtg gggactcgat cacgaccgtg tgctcggtcg actccaagaa
     7681 aagatcaagc gattgaaact gcagatggct gagccggaag cggaaattct tcgattgaaa
     7741 cgcagtggcg atccttcccg gtgatatcga cctttggact gggaagagat atgtggcgcg
     7801 gccagtgcat cgagcttggt cgacggcgcg attgatcgtg acgtgtcatc gggacacagt
     7861 ttgtcggtcg ttggccgggc tagcggagaa tcgcgagccg gacttacgat gctgaattgc
     7921 cgtggtcctg aggggaagag cgagtgcctt tcggcagagc ctccagcaaa cgatctaccc
     7981 acgaggcggg aagcttttcc actgaatcgg caatgcgcga ttgccaccaa gaagaaatct
     8041 cagctaggtc ctctttttga aagcggtgtt cgagaatgtc gaacgtatcg tgccgataca
     8101 gcacgacatc gagcggatat tcgacgctgg tggagctcgt ccgtgtagca tcgaaggcga
     8161 ggaagccgac cttcagcgcg aggtccatgc tggagttata cctcagcacg cgatcgagga
     8221 gcggctttcc gtagccggat tcgccgatga tacagtaggg ggtgccttca ctgacttcga
     8281 cccaattgcc ttgagggtag atcagataaa gtttgtgggt gaggtcgttt tccagttgcc
     8341 cgcccaccag ggcgtggaga ttgaaggtca gtccagcatt atataaggcg cttttatctt
     8401 cctcggccac acggcggatc tgtgcggcaa acacgttgac cgctttgaac agtttgtcga
     8461 acgtttggtc gctctcgctg atggcctcat cgaaataggt cacagctttg tctcgaaccg
     8521 atcgtaatcc tgatgtcatg agaaacatcg agtggcggcc atgttgatgg atggatacct
     8581 ttcccgcggt gatgcattcc gcccctgtcg tcacccgcgt gtcggcgatc ccgaccagcc
     8641 cctcctccac cttcattccc aagcaaaacg tcattgctcc tccgtgacgg actctgtgta
     8701 gtgaactcgg cgaggcacga agaacgtttc agaaatagcc tcgcctactt ggttgagtcg
     8761 tgcttgaaag tggtccacaa attcatgcag accccttgtg acgcagtctt cgatgtcgac
     8821 aaagtctagt tcggcgcgca ggcgtcccaa tcgtttttcg gccaagtttt gataggatcc
     8881 ccgttcggat ccgctaatcg cgtgcaggga gtcctccgct ttaatgaggc aatagcggat
     8941 tgcccgcggg aaggtgggtt caaggatgag aaaggcggcc acgtcatccg gagcgatgcg
     9001 tccataccgt ttgtaataca tctctaaggc actcgcggat ttcaataaat ccgaccattg
     9061 gatgatgtcg aacggcgtgc cgacttcatc ggcggtcggc aagaggatga aatatttgac
     9121 atcgagaatg cgagaggttt tgtccgctcg ttcgaggagg cggccgagcc gtgcaaagtg
     9181 ccaaccctct ccatgggaca tggtggcatc ggtgattccc cacagtagat ggctcgcggc
     9241 tttgacgtcg gccaaaaatg cgtggaggtc atggtgcgac atcccgcgag tgacggcgtc
     9301 cctgaccatc aggtaaaagc ggttgacctg ttcccacatt tcactgggga tgatttcctt
     9361 gatcgaccgt gcgttttccc gcgcgcccag gagacaggac agaatggaat tctggttctc
     9421 ggcatccgcc acaaggaaat gaagcaccgt ctccttcgtc gcttttccat agcgcgcgac
     9481 aaacatctca tgatcgcccg tcgtcgcgac cagcggctcc cactgctcga ccgtgccgcg
     9541 aggaaggtca agagagagat tcaaattgac ctcgatgaac cgggcatagt tttccgcccg
     9601 ttcgatgtag cggttcaccc aatagatgga actggccact ctgctcagca tcgcggtctc
     9661 cgaccaaggt gttcgcagag ccgccggccg accgatcggc aggaggcgtg tgcgcgcgca
     9721 agactcatga cagcacccac gtatctttgt tgccgccacc ttgggaggaa ttgacgacca
     9781 aggacccttt tctcaaggcc acgcgagtca tgccgcccgg caacacatag aaccctttcc
     9841 cctgcaagac gtacggacga agatcgacat gtcgtccctc gatatggtct tccaccagag
     9901 tcggcgatcg ggagagcgcg agcgtgggct gagcgatgta gttgcgaggg tcggcctgga
     9961 tgcgacgggc acagtcttct cgttcttgct gagaggcctg ggggccgatc atcatgccgt
    10021 acccaccggc ttcgttcgtc gccttgacta ccaactgatc gagatgttcc agcacgtagt
    10081 ctcgatcccg gcgttcccag cagacgtagg tgggcacgtt ggggagtatc ggttcctctg
    10141 ccaagtagta gttgatgatc ttggggacgt aggcatagat cgccttatca tcggcgacgc
    10201 cggtgccggg cgcattcgcg atcgccacgc ggccgttctt ataggcctcc atgacaccgg
    10261 gaactcccag cagagaatcg ggacggaaca ccaacgggtc caaatattcg tcattgatcc
    10321 gccgatagat cacgtccacc cgttgagagc ccttcgtggt gcgcatgtgg accgagccat
    10381 cgatgacgac caggtcactg gcttccacca gttccacacc cattttttga gccagaagtg
    10441 aatgttcgta gtaggcggaa ttgtagctgc cgggcgtgag gatcacgatc gtcggatcgg
    10501 ggaggtccga caaggaccga agggtttcca gcaggcggct tgggtattca tcgaccggtc
    10561 tgacgcgata ggcctcaaac agctctggaa aagttcgttt catcacctgt ctggcttcca
    10621 gcacatacgc caccccggac gggcagcgca tattatcctc aaggacataa tagcggccgt
    10681 cgctgatgcg caccaggtcg atcccggcga tgtgacacca gatgccgcga gggggattga
    10741 gcccccagca ggctttcaga aaccctttgc tggaatagat cagttcgctc gggatgacgt
    10801 tatctttgag gatcttttgc tcgtgataca catcatcgat aaataggttt agtgcgcgca
    10861 ggcgttggcg aaggccagtg tcgatgagcg tccaatccgg tgcctccaca atccgcggta
    10921 cgatgtcgaa gggaaagatc tgctcgtgcc cctcgtcgct gccgtacacg ccgaacgtca
    10981 tgcccatgtt caggatgacg cgttccgctg cttgctggcg ctttctaagt tctccatcgg
    11041 ggagcgatgc aaacttccgc agcagtaacg cgcttccccg ccggggccgg ccgatccctt
    11101 caaataattc gtcgtagaac tcgcctgggt cgtaggtgga aaacttcatg tcaggtgtca
    11161 gtgccttccg tcgttcttcg gacggctcgg cagggagcgc cgtttccccg cggcgtatcg
    11221 acgctgccgt ccgaacctac tccttgctca ggggaatttc tatcaccatc ccgcccatga
    11281 cgtcatcgat cttgaaatcg tgtttcgggc tttgcgggtg cgcattgtgg caagcgatgc
    11341 aggcctgtgt tacggcgcgg tcggcataga cggcttggaa ataagactga ctaccgatgg
    11401 tgacggtgcc ggaaacagac cgttcggggt ggagtcgcac ggcttccagg ctgtttcgct
    11461 ccgagttgct cgccggtgcg ttctgagggt tgatcggcca caagctcatg aggcggtagc
    11521 gcaccctggt tccggtcaag gttgccaatt cactggcctc ccgcagaaat tgggccggca
    11581 gcggcaacgt ggtcttgtcc ttccgccagg tttccgatgc gggtgtaccg ccctgtttct
    11641 gcattcgctc gacaacatgg atggtgtaaa acgttcggtc cgcttcaatc actgcatgta
    11701 ggtagtcagc gactacttcg ggggggattc ccggcagctc tgctgctagt cctgttttcg
    11761 cgagtccgct cgccaggatc aggcacgttg cgacgaacgc gagcttccct cccatggtcc
    11821 accctccttc tcataagtcg aaccggcctc gcaacgctca gcataggccg attgtcgtcg
    11881 agaggcaatc ggtcggcctt ctacgattgc attcggttgc cgcgctgccg tagcgtaaca
    11941 gcgttatgca aacggaattt gtcatctctg ccggggagca gcccaaacgg ttggacgtat
    12001 tcctcgtgca ccgcgagccg aagctctcgc gcgcggcctt gcagcggatg attactgcgg
    12061 gctgggtgcg ggttaatagt ctgatcgcca aatcgagtca gcgaatcaaa gcgggagatg
    12121 tcatcgtctt tgatgcgcct caggctgccc cgttacggat cgatggcgag acaaaacagc
    12181 tggagattct gtttgaggat catgcgtgcc tggtgctgaa taagcccgtc gggatcgtgg
    12241 tccacccgtc ccctggacat tggtccaaca cgctgctgaa tgcattacta gcagtgtgtt
    12301 gacaaacccc tcttgccttc gctgtgccat atggtagaag gg
//
LOCUS       DAORRQ010000090        12232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_249_length_12232_cov_47.946374, whole genome shotgun sequence.
ACCESSION   DAORRQ010000090 DAORRQ010000000
VERSION     DAORRQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249_length_12232_cov_47.946374"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /gene="pal"
                     /locus_tag="PK224_18545"
     CDS             <1..580
                     /gene="pal"
                     /locus_tag="PK224_18545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRB17803.1"
                     /translation="TGGMDAPSATFPDLSLSSKPDDLETGGLRGFDSVSGGKAPSEER
                     LGGGGTMLAKVEPSESTARQIEDIRREQAKEQAASAEAGLRDVFFGYDSWTITEEGRQ
                     SLTQDAQWIKANAGALVKIEGHCDERGTLAYNLVLGEKRAKAVRNYLVELGVGANRLA
                     VVSYGKERPFCNERSESCYQQNRRGHVVVRSK"
     gene            645..2321
                     /gene="ybgF"
                     /locus_tag="PK224_18550"
     CDS             645..2321
                     /gene="ybgF"
                     /locus_tag="PK224_18550"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRB17804.1"
                     /translation="MDSGWPDRSEMVTNPSFGRLGAVGGKAAPARLGVLVLIASGLAL
                     LSGCVAQQADLKQTERELQRRIKQQTEEQAQTRARQNQEIVSLREQEIPKLSGDVGEA
                     IHRTKALDARLDDLMAKLASQDSKLSQRMSEGEKRSAEESKRLGWVEKQLVDQDALLK
                     GERDRNRAEFAAVTARLDQVTAHMDAIQKNVLDAMQKTTTVLAQKVDSRLDDQQKVLH
                     GLETRSQNVAQLDSQNKVLADQVSRLNQALVDFKQALGNLGERVVQQDQAVKHLAASL
                     DQDTVALNKRTDALAGKIEADNKVTAEHLNEVNRSVTSVAKALENAGGKFVSREDDHE
                     RRIDETTRELSHVQAQIQTLDKNLENQHAFLKQVEQHLSALRVSASQRAEQAPAVTEV
                     APLAQSQAMPPPEAVSPPTSSPVPSSGSMSRSENRSAALMADRESYERTLTRFKDGDL
                     DGARQGFAEFLLQHPHSDLAPNARFWLGESYYGKKDFSRAIDAYDQVQLNHPASEKVP
                     AALLKKGYAYLALKDRKKAASALKQVIDLYPKSPEANKAMDKLNQLKELH"
     gene            2324..3103
                     /gene="ybgF"
                     /locus_tag="PK224_18555"
     CDS             2324..3103
                     /gene="ybgF"
                     /locus_tag="PK224_18555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRB17805.1"
                     /translation="MKLRFVEALQVGAGLTCGLVLAGCAKHADFLEIRDQVSIIARTQ
                     DQEQKRFEAMQRRLESLERVREPEGGKLRLDDALARLQKLEGRLAKIEETQLAQAASI
                     RSDLALAEANRQARVSKPSGPIEPATIMPGVPSITPTSAFNLAYNDYLNGKFDLAVTG
                     FQRFIKDFPSTSLTPNAHYWLGESYYGQKDYIRAMQSFEHVVNEYAGNEKVPAALFKL
                     GLSAAETGDTAKSRKYLKRVIEEYSTSDEAKLAKAKMAEIR"
     gene            3176..4975
                     /gene="mutL"
                     /locus_tag="PK224_18560"
     CDS             3176..4975
                     /gene="mutL"
                     /locus_tag="PK224_18560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRB17806.1"
                     /translation="MDIASSVGKIQVLPSDVIGRIAAGEVVERPAAVVKELVENSLDA
                     GSSTITVEVKDGGLGLIRVTDDGEGMSRRDASLAFERHATSKLQSDQQLGTICTMGFR
                     GEALPSIAAVSKVRLTTLSRQEQVGTQLWLTAGTITRVEDAAAIPGTSIEVSELFYNT
                     PARRKFLKSTTTEFSHISHVVQQAALASPQVHVRLIHNGYEVFALPAVSSPRDRVLQV
                     YRAAFGDRALAVDVQQNGLSLRGFIIDPVRARAGRTPQELFVNRRPIKNSTVQHAVVD
                     GYSSFLAKGHAPLFVLFLDVEPQRVDVNVHPTKREVRFADNEQIHQLVRSAVRHTLGR
                     AHIESSMAGAAHAHPDGNGVSNVAIPVEETGVEPAALSYPSFAPLNVPVPPTPSAESQ
                     TSFVGEGGTPYQSGEAVDIVPLGQMHRTYVIAQVGDELQVIDQHTAHERVLFERLLRA
                     WQGKSLPSQPLLLPEPLELPVQQALILQRHLAELERLGLLIEPFGPSSFLIRSLPVML
                     GHPDLAALVQDLIDDLEQWDSISSLETKVKPILASLACHGAVRAGRAMALPEIRQLAQ
                     DWVAEGSIMTCPHGRRVAFRLSGDELARLFDRA"
     gene            4993..6024
                     /gene="miaA"
                     /locus_tag="PK224_18565"
     CDS             4993..6024
                     /gene="miaA"
                     /locus_tag="PK224_18565"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRB17807.1"
                     /translation="MVRLSESQRASRPLVLLVGPTAVGKSEIGLRLAHALDTDVLTAD
                     SRQVYRGMDIATDKPTPEQRQGVPHRLIDLVDPDEPFNAGQYRRLALQEIQRLYGAGR
                     LPLIVGGTGLYVRTLIHGLCDAPRADQAYRESLLQEARAQGGYFLHTELTRVDPESAA
                     RLHPHDEVKIVRALEVHHLSGQRLSDMQRQHRFAEQEFSVLLIGLNRNRAQLYRRIDD
                     RVESMFARGVVEETERLLADGYGRELGAMKGLGYQQVAGYLAGEYDRAEALRLLKRDT
                     RHFAKRQLTWFRKEPGLRWCELTEQDRSEDVAGRLLEMIRSFLQDLAPRRPEGMPYPS
                     LTMEQESTA"
     gene            6021..6920
                     /gene="mtnP"
                     /locus_tag="PK224_18570"
     CDS             6021..6920
                     /gene="mtnP"
                     /locus_tag="PK224_18570"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRB17808.1"
                     /translation="MTESKKAGQAAIGIIGGSGLYDIEGLERVREVRVRTPFGAPSDA
                     IRVGVLGGIRVAFLSRHGRGHRLSPSSINYRANIYALKSLGVTQVISVSAVGSMKESI
                     RPGDVVLPDQFIDLTKRRVSTFFDEGIVAHVGFGEPVCSSVADVLEQAGRAVGARLQR
                     GGTYVCMEGPQFSTKAESRLYRQWGVDVIGMTNMPEAKLAREAELCYATVALATDYDC
                     WHDTEEAVTVEAILATLHKNVALAKQLLKAALPKLAPDRTCVCHQALRTAIVTAPDCI
                     SATAKRRLNLLIAPFVRTRKGKR"
     gene            6924..7841
                     /locus_tag="PK224_18575"
     CDS             6924..7841
                     /locus_tag="PK224_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRB17809.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEVTEVLGGSATYFSTAASYFTSVD
                     LIAVVGEDFPEQHVAFLKSRKIDLAGLERRPGATFRWKGAYSHQLNEAQTLDTRLNVF
                     ETFRPKIPAQYSSPDVLFLGNIDPNLQLDVLQQVKRPALVACDTMNFWINGKRDALWN
                     VLQHIDILIINDGEARALGEDTNLVKVAKKILARGPKHLIVKRGEYGVLMFNDKQVFG
                     APAFPLDDVRDPTGAGDTFAGGFLGYLAATGNRSPEAMRQAIIFGSVMASFTVEAFSL
                     DRLRILDYKEIEARFKEFKRLTHFEDIEA"
     gene            7838..8539
                     /locus_tag="PK224_18580"
     CDS             7838..8539
                     /locus_tag="PK224_18580"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17810.1"
                     /translation="MMTTRREQDHAAHTASVGAGARWLVLLLGLCVLSGCANEENLRK
                     SKGFYQEGVARLSSDQQQAYVSFQKAVKLNPDNKEAHYGLGHIYSSQGRFKLAEESFR
                     EAIRIDGDYAEANTYLGQVLANQDRWKEAIAAYRQALSNPLYPTPDLARFHLGKALMH
                     EGDLQGAMEVLEDATTVTPPNVPPAMLQLELARVYHKLGFDVRAREALAKVSNLDKGG
                     EQAAAAQELLGQLKP"
     gene            8549..9502
                     /locus_tag="PK224_18585"
     CDS             8549..9502
                     /locus_tag="PK224_18585"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRB17811.1"
                     /translation="MESVGEFFRQVRETKGLTVDEVASKTRIRTDFVKALEEGNFAKL
                     PDQVFARGFVRSYARSLGLDEEDAIHRFVQSAGAFYEKQGERERLRQRQVEEERRRKA
                     NRKAVGIAIAVAIATLVFLLSREQSSTLVRRSGSDQPSPSKKSAPFAKDTREAPRQGL
                     ELPPPIVPPATKPVEPVAVPSKSAQEKVAAAVPPPPAPPVKPAPVQPEQVAAQPSFSG
                     SDGPLAGLSVDGPAGPDGPLVLDLDATELSWVVVQVDSGSPQEALLRPGQKAQWKAQD
                     QFTVTLGNAGGVRAELNGKPQKPFGPSGKVVRDVVLKRSGL"
     gene            9939..10391
                     /locus_tag="PK224_18590"
     CDS             9939..10391
                     /locus_tag="PK224_18590"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRB17812.1"
                     /translation="MGYFSKLVGVCAAVTLLSVAVVGAEEKDPLKPRVAPDQMADAKA
                     MKNPVASTPESIAKGKALYEGKGTCFNCHGKEGKGDGPAGAILNPSPRNFTNCKFHKK
                     RKDGELFWVIKNGSAGTGMVSLIPAAINEEEAWMIINYERSFCKGGEE"
     gene            complement(10603..>12232)
                     /locus_tag="PK224_18595"
     CDS             complement(10603..>12232)
                     /locus_tag="PK224_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17813.1"
                     /translation="TGGMDAPSATFPDLSLSSAGKPDVTVRIRMLDPRFKIQPDALYD
                     RAPAELSLDALAEFFDAAGAPLAERPLQSTRKERLQLELTQQRCDYIVDPLLQDASTV
                     LATQFMQEARVLLDPTHAATAAAPPASVVPTEAAQKPAGIVPANSTTTMASSLSFKAT
                     LLDENSNLILEGGERIRVRVDIVNTGSQAVQPMTVQLTGPPALIAQFPATKLSTGTIE
                     TGGSKSLEFIATLPQSLNPQQAEFQVSLASGTGQPVPAPQTLLASVRPTGITTDDVDQ
                     VPAPTTGGQRSGDYLVSIGISSYREQHIGGRKYAALDAEMVATYLQALGGVPKNNVRL
                     LQDWSALRPDIEEALLDWLPSKLTQDSLVTVYFAGQAVVSPTGDTFLIPYDGSLGSTS
                     RLYPLKDLEAALERLKAKQILFVFDGTVLKNGGEGRSKVAAPKWTGTSGKVLHLIGTT
                     GFGKSLESDTLRHGLFTYYLLRGLRGEADLNRNGEVTIGEVTDYLTRKIPTAARNTFK
                     QEQQPQVFPALRAPDKGMDAVLTRPPTIPATDKP"
BASE COUNT         2452 a         3346 c         3960 g         2474 t
ORIGIN      
        1 aaccgggggg atggatgctc ccagtgcgac attcccggac ttgtctctct ccagcaagcc
       61 ggatgatttg gagaccggcg gattgcgcgg gttcgattcg gtatcgggtg gaaaagcccc
      121 gtccgaagaa cgcctgggcg gcgggggcac gatgttggcc aaggtcgagc cgtcggagag
      181 cacggcgcgt cagatcgagg acattcgccg cgagcaagcc aaggaacaag cggcatccgc
      241 ggaagccgga ttgcgcgacg tgttctttgg ttatgacagc tggaccatca ccgaagaggg
      301 acgtcaatcc ttgacgcagg atgctcagtg gatcaaggcg aatgcaggtg cactcgtgaa
      361 gatcgaaggc cattgcgacg aacgcggcac gctggcctac aatctggtgt tgggcgaaaa
      421 gcgcgccaag gccgtgcgga actatctggt cgagcttggt gtgggcgcca accggttggc
      481 agtggtgtcc tacggcaagg aacggccatt ctgcaacgaa cggagcgaaa gctgctatca
      541 gcagaaccgt cgcggccacg tggtcgtgcg gtcgaaataa ctgttccgat cagggatgat
      601 gtgacatgat tctccggagc aacgccggtt aagggagacc tgtcatggac agtggatggc
      661 cagatcgaag tgaaatggta acgaacccat cattcgggcg cctcggagcg gtcggcggca
      721 aagctgcgcc ggcccgactc ggtgtgctcg tgctgatcgc gtctgggttg gccttgttgt
      781 cgggatgtgt ggcgcagcag gctgacctga aacagacgga gcgtgaactg cagcgtcgta
      841 tcaagcagca gacggaggaa caggctcaga caagggcgcg tcaaaaccag gaaatcgtct
      901 cactacgtga gcaggaaatt cctaagttga gtggcgatgt gggggaggcc attcaccgca
      961 cgaaggccct ggacgcgcgg ctggatgact tgatggcgaa attggcctct caggattcga
     1021 agctcagtca gcgaatgagc gagggggaga aacgctccgc tgaagaaagc aagcgactgg
     1081 gttgggtcga gaagcagctg gtcgatcagg atgccttgct caaaggcgag cgggaccgga
     1141 accgggcgga atttgcagcg gtgacggctc gcttagatca agtcaccgct cacatggatg
     1201 ccattcagaa gaatgttctg gatgccatgc agaagaccac gacggtgctc gcgcaaaaag
     1261 tcgattcgcg cctggacgat cagcaaaagg tcttgcacgg tctcgaaacg cggtcccaaa
     1321 atgtggcgca gctcgattca cagaacaagg tgttggccga ccaggtttcc aggctcaacc
     1381 aggcgttagt ggatttcaag caggcgttgg gtaacttggg ggagcgtgtc gttcagcagg
     1441 accaggccgt gaagcatctg gccgcgtcgc tggaccagga cacggtggcg ttaaataaac
     1501 ggacggatgc cctggcggga aaaatcgagg cggacaacaa ggtgaccgcg gaacacctga
     1561 acgaagtaaa ccggagtgtg acttcggtgg cgaaggcgtt ggaaaatgcc ggcggaaaat
     1621 tcgtgtcccg cgaggatgac catgagcgcc gcatcgatga gacgacgcgc gaactgagtc
     1681 atgtgcaggc gcaaattcag accctggaca agaatttgga gaatcaacac gcgttcttga
     1741 agcaggtcga gcagcatctg tcggccttgc gcgtcagcgc gtcgcaacgg gccgagcagg
     1801 cgccggccgt aacggaagtg gcgccgcttg cacagtctca ggccatgcct cccccggaag
     1861 ccgtctcgcc gccaacgtcg tctcctgtcc cgtcaagcgg ctcgatgtct cgctccgaaa
     1921 atcgtagcgc agcgctgatg gcggatcgtg agtcgtacga gcggacgttg acccggttta
     1981 aggacggcga tttggacggg gcgcgccagg gcttcgcgga atttctcctg caacatcccc
     2041 actcggatct ggctcccaat gcgcggttct ggctaggcga gtcctattac gggaaaaagg
     2101 atttttcgcg cgccatcgat gcctacgatc aggtgcagtt gaatcatccc gccagcgaaa
     2161 aagtgccggc tgcgctgctg aagaagggct atgcctatct cgcgctcaaa gatcgaaaga
     2221 aagccgcctc ggcgctcaag caggtcatcg acctgtatcc aaaatctccg gaagccaata
     2281 aggcgatgga caaactgaat caactaaagg agttgcacta acgatgaagc tccgattcgt
     2341 ggaggcattg caggtagggg cggggctgac ctgcggactg gtgttggcag ggtgcgcgaa
     2401 gcacgccgat ttcctggaga tccgggatca ggtctcgatc atcgcccgga cgcaggatca
     2461 ggaacagaag cgattcgagg cgatgcagcg ccgactggag tcgctcgaac gcgttcggga
     2521 gccggagggc ggaaagctgc ggctcgacga tgcgttggct cgccttcaga agctcgaggg
     2581 acgtctggcc aagattgaag aaactcaact ggcccaagcc gcctccattc ggtcggacct
     2641 cgcgcttgcc gaagccaatc gtcaggcgcg tgtctcgaag ccgtccgggc ccattgagcc
     2701 ggcgaccatc atgcccggag tgccgtcgat tacgccgacc tcggccttca atctcgccta
     2761 taacgactac ctcaacggca agttcgatct tgccgtgacc gggtttcagc ggttcatcaa
     2821 ggattttccc tcgacctcgc tgacgccgaa cgcgcactat tggctcggag aatcgtacta
     2881 cggccagaag gattatattc gggcgatgca gtcgttcgag cacgtcgtga acgagtatgc
     2941 gggcaatgaa aaggttcccg ccgcgctctt caagctgggc ttgtcggccg ccgaaaccgg
     3001 cgataccgca aaatcgcgca agtatctgaa gcgggtcatc gaggaatatt cgacgtccga
     3061 tgaagccaag ctcgcgaaag cgaagatggc cgaaattcga tgaccttggg cctgctgcac
     3121 ggtcctctct ccatccgctc atcctcggtt cgaatctcag ggagcgtttc ttcccatgga
     3181 catagcgagt agtgtgggaa agattcaggt tctgccgagc gatgtcatcg gccgcattgc
     3241 ggcaggggag gtggtcgagc gtccggctgc ggtcgtcaaa gagctcgttg aaaacagcct
     3301 ggacgccggc agcagcacga tcaccgtcga ggtcaaagac ggaggcctcg ggctgattcg
     3361 tgtcaccgac gacggcgagg gcatgtcccg acgtgatgct tctctcgctt ttgagcggca
     3421 cgccaccagt aaattgcagt cggatcagca gctcggcacc atttgcacca tgggctttcg
     3481 cggggaggcg ttgcccagca tcgcagccgt atcgaaagtc cggctcacga cgttgtctcg
     3541 ccaggaacag gtgggcacgc agctctggct cacggcgggg accatcacgc gggtcgaaga
     3601 tgccgcggcg attcccggga catccatcga ggtttcggag ctcttctaca atacgcccgc
     3661 ccgcaggaaa tttctcaaat cgaccacgac agagttctcc catatcagcc atgtggtgca
     3721 gcaggccgcg ttagcgtcgc cgcaggtaca cgtgcggttg attcataacg gctatgaggt
     3781 cttcgcactg ccggccgtgt cctcgcctcg cgatcgtgtg ctgcaggtgt atcgcgccgc
     3841 attcggtgac cgggcgctgg ccgtcgatgt tcagcagaac ggtttgtccc tgaggggctt
     3901 catcatcgat ccggtgcggg cgcgcgccgg gcggacgccg caagaactct tcgtcaatcg
     3961 caggccgatc aaaaacagca ccgtgcaaca tgcggttgtg gatggctaca gttcgtttct
     4021 cgcgaagggc catgctccgc tgtttgtgct ctttcttgat gtcgagcccc agcgggtcga
     4081 tgtcaacgtg catccgacta aacgagaggt ccggtttgcc gataacgagc agatccacca
     4141 gctggttcgt tctgctgtgc gccataccct tggtcgcgcc cacatcgaat cgtcgatggc
     4201 aggggccgct cacgcgcatc cggacgggaa cggggtatcg aatgtcgcga ttcccgtcga
     4261 ggaaactggc gtcgagccgg cagccctgtc gtatccatca ttcgcacccc tgaacgtgcc
     4321 tgttccgccg actccctcag ctgagagcca aacctcgttt gtcggggagg ggggcactcc
     4381 ttatcaatcg ggtgaggccg tggatattgt tcccctcggc cagatgcacc gcacctatgt
     4441 gattgcgcaa gtgggcgatg aactccaggt gatcgatcaa catacggccc acgagcgagt
     4501 cttgtttgag cgactgttgc gagcctggca gggcaagagc ttgccgtccc agccgctgct
     4561 gttgccggaa ccgttggagt tgcctgttca acaagctctc atccttcagc gccatctggc
     4621 ggaactggaa cgacttgggc tgctcatcga accattcggg ccctcctcct tcttaatccg
     4681 cagtctgccg gtgatgttgg gccatcctga cctggccgcg ctggttcaag atctgattga
     4741 cgatcttgag cagtgggatt cgatttcctc gctggaaacg aaggtgaagc ccattcttgc
     4801 atcactggct tgccacgggg ctgttcgggc gggccgggcg atggcgcttc ccgagatcag
     4861 gcaactggcg caggattggg tggccgaggg gtcgatcatg acctgtccgc acggccgccg
     4921 tgtggccttc cgtctctcgg gggacgaact ggctcgcctg ttcgatcgtg cataggaaga
     4981 gggagtccgg ggatggtgcg actgtcggaa tcgcagcgag cgtcgcggcc cctggtgctg
     5041 ctggtcgggc cgacggcagt gggaaagagc gagatcggcc ttcggctggc gcatgcgctg
     5101 gatacggatg tgctgacggc agattcgcgc caggtctatc gcgggatgga cattgcgacc
     5161 gacaagccga cgccggagca gcgacagggc gtgccgcacc gcctcatcga tttagtcgat
     5221 cccgacgagc cgtttaacgc cgggcagtat cgccggctgg cgctgcagga gattcagcgg
     5281 ctgtatggag ccggccggtt gccgttgatc gtcggtggca caggactgta cgtgcggaca
     5341 ctgattcacg gattgtgtga tgccccgcgc gcagatcagg cctatcgaga atcgttgttg
     5401 caggaggccc gcgcgcaggg cggctatttt ttgcacactg aattgacgcg tgtcgatccc
     5461 gaatcggccg cacggctcca tccccacgac gaagtcaaaa tcgtgcgggc gctcgaagtc
     5521 catcatctgt cgggacaacg cctgtcggat atgcagcggc agcatcgttt cgccgagcag
     5581 gagttttcgg tgctcctcat cggtctgaat cggaatcgcg cgcagctcta ccgccgaatc
     5641 gacgaccggg tcgagtcgat gtttgcgcga ggcgtggtgg aggaaaccga acgactgctg
     5701 gccgacgggt atgggcggga gttgggtgcg atgaagggac tggggtatca gcaggttgcc
     5761 ggctatctgg ccggcgaata cgaccgggct gaggcgctcc gcctgttgaa gcgtgatacc
     5821 agacatttcg ccaagcgtca gttgacatgg tttcggaagg agcccggctt gcgctggtgt
     5881 gagctcactg agcaggatcg gtcggaggat gtagccgggc gtctgcttga gatgattcgt
     5941 tcgtttctcc aggatctagc acctcgacgg ccggagggaa tgccgtaccc gtcgcttacc
     6001 atggaacagg aatcgaccgc atgactgaat caaagaaggc cgggcaggcc gcaatcggta
     6061 ttatcggtgg cagtgggttg tacgacattg aaggactgga gcgggttcgg gaagtgcgcg
     6121 ttcgaacccc gttcggcgcg ccgtcagatg ccattcgggt cggggtgttg ggtggcatcc
     6181 gtgttgcgtt tctctcccgg catgggcggg ggcaccggct cagtcccagc agcatcaact
     6241 atcgcgccaa tatctatgcg ttgaaatcgc tcggggtgac acaagtgatt tcggtcagcg
     6301 ccgtcggcag catgaaggaa tccatccgtc ccggtgacgt cgtgctgccg gatcagttca
     6361 tcgatctgac gaaacgcaga gtgtcgacgt ttttcgacga gggcattgtg gcgcatgtcg
     6421 gattcggtga gccggtctgt tcgtcggtgg cggatgtgtt ggagcaggca gggcgagccg
     6481 tgggggcgcg gcttcaacgc ggtggtacct acgtgtgtat ggaaggaccc cagttttcta
     6541 cgaaggcgga gtcccggttg tatcgccagt ggggggtaga tgttatcggt atgaccaaca
     6601 tgccggaagc caaacttgcc cgtgaagcgg aactctgtta cgcgaccgtt gcgctcgcga
     6661 cggactacga ctgctggcat gacaccgaag aggccgtcac ggtcgaggcc attctggcca
     6721 cgctgcataa gaatgtcgct ctggctaaac aactgttgaa ggcggcgttg cccaagttgg
     6781 cgccggaccg gacttgtgtc tgtcatcagg cgcttcggac cgccatcgtg accgcgccgg
     6841 attgcatctc tgccacggcg aagcgacggc tcaatttatt gattgctccc tttgtgcgga
     6901 cgcggaaagg aaaacggtga agcatgggta aattattggt agtgggatcg gtggcgctgg
     6961 ataccgtcaa aacgccgttc ggtgaggtca ccgaagtgct cgggggatcg gcgacgtact
     7021 tttcgacggc cgcgagctac ttcacctcgg tcgatctgat tgcggtcgtc ggggaggatt
     7081 ttccggagca acacgtggcg ttcctgaaga gccggaagat cgatttggcc ggcttggagc
     7141 ggcgaccggg tgccacgttc cgctggaagg gggcctattc gcaccaattg aacgaagcgc
     7201 agacgctcga tacccgcttg aatgtgttcg agacgtttcg tcccaagatt cccgctcagt
     7261 acagttcgcc ggatgtcctc ttcctgggca acatcgaccc gaatttgcag ctggatgtct
     7321 tgcagcaggt aaaacgtcct gcgctggtcg cctgcgacac gatgaacttt tggatcaacg
     7381 gcaagcgcga cgcactctgg aacgtgctgc aacacatcga cattctcatc atcaacgacg
     7441 gcgaggcgcg ggccctggga gaggacacga atctcgtcaa ggtcgctaag aagattctcg
     7501 cgcgaggccc caaacacttg atcgtcaaac gcggtgaata tggcgtcctg atgttcaacg
     7561 acaagcaggt cttcggggcg ccggcgttcc cgctggacga tgtgagagat cccaccggtg
     7621 cgggcgatac gttcgccgga ggatttctcg gttacctggc agcgaccgga aaccggtctc
     7681 ccgaggcaat gcggcaagcc atcattttcg gcagcgtcat ggcctcgttt acagtagaag
     7741 cctttagcct tgaccgattg aggatcctgg attacaaaga gattgaggcg cgtttcaaag
     7801 aattcaagcg gctcactcac tttgaggaca ttgaggcatg atgacaactc gccgcgagca
     7861 ggatcacgcc gcgcacaccg cttctgtcgg cgcgggggca cgctggctgg tgttgctcct
     7921 gggcttgtgt gtgttgagcg ggtgcgccaa cgaagagaac ctgcgcaagt cgaaagggtt
     7981 ctatcaggaa ggggtcgccc ggctcagttc cgatcaacag caggcctacg tgtcgtttca
     8041 gaaggcggtg aagctcaacc ccgacaataa ggaagcgcac tacgggttgg gacatatcta
     8101 ttcctcgcag gggcggttca agctggccga ggaatcgttc cgtgaggcga ttcgtatcga
     8161 cggtgactat gccgaggcga acacctacct cgggcaggtg ctggccaatc aggatcgatg
     8221 gaaagaagcc atcgcggcct atcgacaggc gctcagcaat ccgctctatc cgacaccgga
     8281 tctggcacgc ttccacttgg gaaaagccct gatgcacgaa ggcgatctac agggggccat
     8341 ggaagtgttg gaagacgcga cgaccgtcac gccgccgaac gtccctcccg ccatgcttca
     8401 attggagttg gcgcgcgtgt atcacaaact cggattcgat gtgcgggcgc gtgaggctct
     8461 cgcaaaggtc tcaaacttag acaagggcgg agaacaggca gccgcagcgc aggaacttct
     8521 cggtcagctc aaaccatagg cggatatcat ggaatcagtg ggtgaatttt ttcggcaggt
     8581 gcgtgagacg aaaggcctga cggtcgatga ggtggcctcg aagaccagga ttcgcacgga
     8641 tttcgtcaaa gcgctcgaag agggaaattt cgccaagttg cccgaccaag tcttcgcgcg
     8701 cggctttgtg cgctcctatg cgcgatcgtt ggggctcgac gaagaagatg ccatccatcg
     8761 gtttgtgcag tccgccggtg cgttctatga aaaacagggt gagcgagagc ggttgaggca
     8821 gcggcaggtc gaagaggaac ggcgccggaa agccaatcgc aaagcggtgg gtatcgccat
     8881 tgctgtcgcc attgcaacgc tggtgtttct gctaagtcgg gaacagtctt cgacattggt
     8941 gcggcgttcc ggatcggacc agccatcgcc gagtaagaag agcgcgcctt ttgccaagga
     9001 tacacgggag gcgccgcgtc agggcttgga acttccgcct cccatcgtgc caccggcgac
     9061 gaaaccggtt gaacccgtgg ccgttccctc aaagtccgcg caggaaaagg tggctgccgc
     9121 tgtgccgcct cctcccgctc ctccggtcaa gccggcgccg gttcagccgg agcaggtggc
     9181 cgctcaaccc tccttctccg gatccgatgg tcctctcgca gggctctctg ttgatggacc
     9241 cgctgggcct gatggtccgc tcgtgctgga tcttgatgcc actgagttga gttgggtggt
     9301 cgtgcaggtt gattccggca gtccccagga ggcgttgctg cgccctggtc aaaaggctca
     9361 atggaaggct caggaccagt ttacggtgac gctcggcaat gccggaggcg tccgcgcgga
     9421 actcaacggt aagccgcaaa agccatttgg gccgagcgga aaggttgtgc gcgatgtcgt
     9481 cctcaaacgt tcaggtttgt gatcgggtag tgttctggta cttgcggccg gtttgagtgt
     9541 tcgtgtccac tcacctgcgg ttcactctcc ataatcggtg tcgccgggcg gctcctccgg
     9601 ttccatttgc acatacgaat ggggcgcgca gggaatctct ggggcgcaaa ggtgtcacgg
     9661 ttgaatcgtg gtacgtacct gcccgtgacc tgaacattca gaatcgccac aatttcagcc
     9721 tctttcccca cttctcatcc tggcacgccc cttgagttcc acgtaggtcg gcataggcga
     9781 agagcgggca aggaggtttc cagttccctt gacaagacag aaaacgcgtt gctatagttc
     9841 gcctgttttg tcgcccttgt catacgcttt ttttcatgcc gtccaggata agcgagtcgt
     9901 tgttcacgcg gtgggttcaa cacaaaggag gagttctgat ggggtatttt tctaagttag
     9961 ttggagtgtg tgcggccgtc accttgttgt ctgtggcggt agtgggagcc gaggaaaagg
    10021 atccgttgaa gccccgtgtt gcaccggatc agatggcgga tgcgaaagcg atgaagaacc
    10081 cggttgcctc aaccccggaa agcattgcca aaggcaaggc actgtatgag ggcaaaggca
    10141 cctgcttcaa ttgccatggg aaagaaggaa agggcgacgg ccctgccggc gcgatcctca
    10201 acccgagccc gcgtaacttc accaactgca agttccataa gaagcggaaa gacggcgagc
    10261 tgttctgggt gatcaagaat ggcagcgcag gcaccggtat ggtgtccctg atccctgcgg
    10321 ccatcaacga agaagaagcc tggatgatca tcaactatga gcgcagcttc tgcaagggtg
    10381 gagaagagta gtcgaacgtt tcggccgatc ggctgaatca atggggggcg tcgagggtat
    10441 cctcgacgcc ccctttcttt tatgtgggaa gtgcccgttt gcgaatccat ggagagagcg
    10501 ggcgaggagg gacggcgcca ctccctcctg gatctagcta ctgtatgtag cgataggttg
    10561 ttggtattcg ggcgaggcag ttggcaggaa ttgaatggtg cttcagggtt tgtcggtcgc
    10621 gggtatcgta ggtggtcttg taagtaccgc gtccatcccc ttgtcggggg ctcgtagtgc
    10681 agggaacact tgcggttgtt gttcctgctt gaaggtattg cgagctgccg tcgggatctt
    10741 tcgggtgagg taatccgtga cctcgccgat cgtcacctct ccgttccgat tgagatccgc
    10801 ttcgccacga aggcctcgca agaggtaata ggtgaacaaa ccatggcgca acgtatcgga
    10861 ctccaggctt tttccgaaac ccgtggtgcc gatcaggtgt agtacctttc cgctcgtgcc
    10921 cgtccatttc ggtgccgcga ccttcgatcg tccctcaccg ccgtttttga gaacggtccc
    10981 gtcgaatacg aacaggattt gcttcgcctt caatcgttcc agtgccgcct ccagatcttt
    11041 cagcggatac aggcgggagg ttgatcccag ggagccatcg tagggaatca gaaaggtgtc
    11101 tccggtcggc gagacgacag cctgcccggc gaagtacacc gtgacgaggg agtcctgcgt
    11161 gagttttgat ggaagccaat cgagcaacgc ttcctcaata tcggggcgta aggcgctcca
    11221 gtcttgcaac aaccgcacgt tattcttggg cacccccccc agcgcctgta aataggtcgc
    11281 gaccatttcg gcatccaggg cggcgtattt gcgcccgccg atgtgctgct cgcgatagct
    11341 gctgatgccg atggacacca gatagtcgcc ggatcgctgt ccccccgtcg tcggagcggg
    11401 aacttgatcc acatcgtcgg tagtgatgcc ggtcggccgt accgaggcca gcagcgtctg
    11461 aggtgcgggg acgggttgtc cggttccgct tgccagggag acctgaaatt cagcctgctg
    11521 cgggttcagc gattgcggca gggtggcgat gaactcgagg gatttggagc cgcctgtctc
    11581 gatggtgccc gtggataatt tcgtagccgg gaattgtgcg atcaacgccg gtgggccggt
    11641 gagctgaaca gtcatgggct gcaccgcctg ggagccggtg ttgacgatgt cgactcgtac
    11701 gcggatccgt tcgccgcctt cgagaattaa gttgctgttt tcatcgagaa gggtggcttt
    11761 aaatgacagg gaggatgcca tcgtggtggt cgagttggca gggacgatgc cagccggttt
    11821 ctgggcggcc tcggtaggca cgacggaagc gggtggggcc gcggccgttg cagcatgcgt
    11881 ggggtcaagc agcacacgcg cttcctgcat gaactgcgtg gccagcacgg tggacgcgtc
    11941 ctgaagcagg ggatcaacga tgtagtcaca gcgttgctgg gtgagctcaa gctgcagccg
    12001 ctccttgcgt gtggattgca agggccgctc ggccagcggt gcgccggctg catcaaagaa
    12061 ctctgcgagg gcatccaggc tgagttcggc gggggcacgg tcatagagcg catcgggttg
    12121 aatcttgaat cgagggtcca gcatgcgtat ccgcaccgtc acgtccggtt ttccggcgct
    12181 ggagagagac aagtccggga atgtcgcact gggagcatcc atccccccgg tt
//
LOCUS       DAORRQ010000091        12132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_252_length_12132_cov_15.552703, whole genome shotgun sequence.
ACCESSION   DAORRQ010000091 DAORRQ010000000
VERSION     DAORRQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_252_length_12132_cov_15.552703"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PK224_18600"
     CDS             <1..759
                     /locus_tag="PK224_18600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17814.1"
                     /translation="LLEKSFITKAEYDIAGARVRRAAAAVRSAEASVMAAEAERKAAE
                     VQLENTNIRAPFDGIVVKKLAELGEVVSPMTASVRSGGSVVTIVDPASVMVDAEVSEA
                     MINRVQAGQSAEIQLDSVPGHRYRGEVVQVMPIADRAKATILTRVRFFELDERVRAEL
                     SAKVTFGPKPGSVEEDRESWGVPPTAVVSRDGRQIVFVVKDAVVTERVVEAVSTSGPI
                     QAVHGLLTQADEVVVAPPADLRAGAPVTIRRRTL"
     gene            769..1452
                     /locus_tag="PK224_18605"
     CDS             769..1452
                     /locus_tag="PK224_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB17815.1"
                     /translation="MYDPVVTLQRISKTYSRAGTDVSVLRDLSFTIPAGTFLAIMGPS
                     GSGKSTLLNVMAGIDRPTSGSVVVAGTRLDDLSEGDMARWRARHIGYVFQSYNLIPVL
                     TASENVELPLLLTHLSRQERREHVKTALRLVGLGERMDHYPRQLSGGQEQRVGLARAI
                     VSDPTMILADEPTGNLDRESAEDILTLFGRLNRELGKTIVMVTHDPRAAERAQMIRHL
                     EKGVLESPP"
     gene            1449..2615
                     /locus_tag="PK224_18610"
     CDS             1449..2615
                     /locus_tag="PK224_18610"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB17816.1"
                     /translation="MTLGRLVLRNTLRRPVRLALTVWGLAMVVLAFGLIRLTLDEWQG
                     AAKAASKNRLITVHAMSGALPLPLAYRDRIAVLPGVRSVHYGVEFGGIYKDPKQPLAS
                     FAEPAATLLTTYPEILLSERERLAFVQDRRGCIVGLSLAERYGWKLGDVIPLTGTNYP
                     GQWEVIVRGIYRSSNLMIWSDQELFLQWEYLNEQVKTTQPDRADQVSWYAIDVKEGSN
                     PRAVALALDALFANSFAETRTELEQAFIAGWVERSSALLYGLEAVSAVINGIALLVLV
                     NALAMAIRERTREYAVIKTLGFQPRHFCILVLGESLLLALSGGALGLGLLVPASRLYA
                     QMTQSNGYVATYAVSMETVGLCLGMMVLVALGAAIWPAVRLIRMTTLDGLRHAG"
     gene            2814..3986
                     /locus_tag="PK224_18615"
     CDS             2814..3986
                     /locus_tag="PK224_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRB17817.1"
                     /translation="MVRWGLYSLRNLWARRVTTALTVVGLGLVVFVLLAVLMLAHGLE
                     RTLGKTGDPENVIILRKGALSEIGSSMSREEIRAVAAQPDVRLTADGRPLAVAEVGLQ
                     LTLRKGQGGSLTSVTLRGTAPEALAVRPAILLIQGRLWKPGTTEIVVGASIARQFPDA
                     RVDRVLHFGNRAWTVVGVFEAGGSGFDSEVWGDAEQFLATFHRTTFSSLTLRLAQPAT
                     LPAFKDRIERDPRFKFSVKREPEYYEGKAEGLARMIRVTGLFLTVVFSVGALLGATMT
                     MSASVAQRTTEIGTLRTIGFTRMDILQAFLLESMALGAIAGLLGVAAAVGLQSMTIST
                     MNFDTGTELVFGFHLSGEMIAIALVFAMLMSIVGGLIPATRAARLVIVQALGERTF"
     gene            4135..4467
                     /locus_tag="PK224_18620"
     CDS             4135..4467
                     /locus_tag="PK224_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17818.1"
                     /translation="MNRRTSQTSVAGLALGILWVYMVLALVAPGCSFAHATPSDSHHQ
                     HSGTESHSTLCSWACQATSDVGPISQGDVGTAWVAQSHTLITPPLLLTSGDLSSLHAR
                     APPIPVRG"
     gene            4838..5086
                     /locus_tag="PK224_18625"
     CDS             4838..5086
                     /locus_tag="PK224_18625"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRB17819.1"
                     /translation="MVEQPLLRVKEAAQLLQVNKWTIYRWIDEGRLNATKIGGGSLRI
                     FRVSVTALVEGNRTDPRPQEPTPHLKAVPLGGRRAMKR"
     gene            5217..5768
                     /locus_tag="PK224_18630"
     CDS             5217..5768
                     /locus_tag="PK224_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HRB17820.1"
                     /translation="MMVREILSLIVVMGVAWNGGIVTTVAEAGAAPKDGEIALPADYK
                     TWPKFLSEVQREDAKQVRELFVSPAGAKACPASAFPQGTQLVMELYKAKAEGEALAKG
                     ADGKLVKGDLLKVFVMEKNEGWGQDVPDNLKNGAWVLGAYGPDGKALAEDFTKCRACH
                     APLAQKDFVHRYDEFCAQGHKAH"
     gene            6228..7382
                     /locus_tag="PK224_18635"
     CDS             6228..7382
                     /locus_tag="PK224_18635"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17821.1"
                     /translation="MADCTKKTPRWLLMALVVGMALCGVDGGWANEPDAWKQLFTDGV
                     KAREAAHYAEAEQLLQRALREAEQAGPDDARVATAANNLGLLYHDQGRLQEAQSLYTR
                     ALAMWEQHLGPEHEDVAAALNNLAEIAHAEGDWGRAEPLYERVLAIERKVLGAAHPDV
                     AISLNNLAELYRKQGRETAAESMYHLALILMEEARGTDHPDLGPVLNNLAVLYKGRGL
                     YAWAEPFYLRALKMRRLRFGDEHPAVAMSLNNAACLYQAEGLYASAQRYFDEALAIAE
                     RTSGPQASLTGTIVGNMAFLADEQGLWTQAQLLYQRALVVQQAQLGLHHPTVGLLVER
                     YARVLDTLGQPVEAGLFAARAASIRARVQPGVVRQGSAMRQGPAVGELTR"
     gene            7414..7941
                     /locus_tag="PK224_18640"
     CDS             7414..7941
                     /locus_tag="PK224_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17822.1"
                     /translation="MPVSAAVASPRRAEMAQGAQHRAGLSVAVVRLLAVALDISEGWS
                     PAPPAATLLIPFGYRDWPSLTSTVESPGGRKRLRFYVCPKALLTPDYESFPVGTTFVV
                     ESEPYPEGPGSYRARPSVFAMEKCAGLTMDGAGTRQYESWMYASWDSPAQQAMADRGR
                     CGVCRLPWLTPADVR"
     gene            8167..8469
                     /locus_tag="PK224_18645"
     CDS             8167..8469
                     /locus_tag="PK224_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17823.1"
                     /translation="MTVLRSGMLAFAVSALVATAPVGYALADGGGHVKETIKHAKEGV
                     EHEKEAIKHLEESVKASNDPHAKEALEHAKEAVKHAEESLAHAEQASSHKGKAKGK"
     gene            8725..9069
                     /locus_tag="PK224_18650"
     CDS             8725..9069
                     /locus_tag="PK224_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17824.1"
                     /translation="MRKLFVAVVQILVLAGGSGVWAADDSLERLLRAQAFNREFEGFD
                     SYYVKIEADQPQPDGSHEVLAVASGKFSDNTKRMKVLFLIVDGHIVGGQVLEGTGLPP
                     CLAQEHRPSSSL"
     gene            9086..9565
                     /locus_tag="PK224_18655"
     CDS             9086..9565
                     /locus_tag="PK224_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17825.1"
                     /translation="MLSRIAVLFMGVLGVLASGTSALALQAGGVEVGDLETGSVVGQP
                     FKELVVDAELYAVEIGNMKAWYPPTTVIDFKVRPGRPLLIKVTNTTATERGFQMIDTV
                     NAASPFVLKAEVRLKPGETKYIGVPTSDLFNQTQGNTLEYRDQLNPKQPGGKLLMIK"
     gene            complement(9675..10226)
                     /locus_tag="PK224_18660"
     CDS             complement(9675..10226)
                     /locus_tag="PK224_18660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HRB17826.1"
                     /translation="MRVKLLQGIGILWFAAILAACASSGDMHESADGGPHVPPFSQIK
                     AAPDSFKGQTLVLGGQVLAARRMKDGTRIEVLQLPLNDSQQPTLDLMKSQGRFVAVQR
                     DFLDPATIPQGTFLTITGELTGSMTLPLDETDYTYPVIDIKSFRTWIPSQDSTMSRIR
                     PYPYYSPFWHPYWGPYGRFPYYW"
     gene            10323..10967
                     /locus_tag="PK224_18665"
     CDS             10323..10967
                     /locus_tag="PK224_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRB17827.1"
                     /translation="MAKRRGWRGSLLMGLMAVAALGFVGTAQAGKAELKGNFQVLSDE
                     KSTHKPGKVQLTEFADFYCPHCHRFDGEGLAILEKEFGHKLEAVMVGYPVIPGKLPTA
                     FDMYEQAKTMGKGAEMKRALFRTIHKDKIGIIDKAIREVLIREVGLDPAAFEEGLASA
                     KPAKAFEDGRKWGDRIKIQQTPTVLLDGNIKVEQIDPENLKLIIHSILDGDGKK"
     gene            11044..>12132
                     /locus_tag="PK224_18670"
     CDS             11044..>12132
                     /locus_tag="PK224_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRB17828.1"
                     /translation="MATDPICGMTVEPATAAGHFDYDGRTYYFCSTHCLDRFRATPNQ
                     YVKTASVATAAMPAAGRRSLPMMQAMPEQQPVAGGERDPVCGMTVQPATAAGSHAHAG
                     KTYYFCCQGCLEKFRADPVRYLSPVPAVSPTASRQFGGKSLPMLGSATPDKAVTGVID
                     PVCGMTVEPATAAGSFDYQGTTYSFCCQGCLTKFRADPQRYLAPASAQSTAPAAPPPP
                     GTKYVCPMCPEVLEEKPVPCPKCGMALEPDSIQPLPTKTEYVCPMHPEVVKAEQGACP
                     ICGMALEPRTVTVEEELNPELVDMARRFWVGLGPAAAVFVLAMSPMIPGHPIQHLVTD
                     MQSAWVQFLLSTPVVLWAGWPFFQRGWAS"
BASE COUNT         2236 a         3405 c         4119 g         2372 t
ORIGIN      
        1 ctgttggaga aatcgttcat caccaaagcg gaatacgata tcgccggggc gcgagtccgg
       61 cgcgcggccg cagcggtccg atcggccgaa gcgtccgtga tggcggcgga agcggaacgg
      121 aaagcggccg aggttcagct tgaaaacacg aacatccgcg cgcccttcga cgggattgtc
      181 gtgaagaaac tggcggaact gggcgaagtc gtctctccga tgaccgcgtc ggtccgttcc
      241 ggcggctccg tcgtcaccat cgtcgatccc gcgtccgtga tggtcgatgc cgaggtgtcg
      301 gaagccatga tcaaccgcgt ccaggccggg cagtccgccg agattcagtt ggattccgtt
      361 ccgggacacc ggtatcgcgg cgaagtggtc caggtgatgc ccattgccga tcgggccaag
      421 gcgacgattc tgacgcgggt gcgattcttc gaattggatg aacgggtccg cgccgaactc
      481 agcgcaaaag tgacgttcgg gccgaagccc ggatcggtcg aggaggacag agaatcctgg
      541 ggcgttcccc cgacggccgt cgtgagtcgc gatggccgtc agatcgtatt cgtcgtcaaa
      601 gacgcggtgg tgacggaacg cgtggtggag gccgtctcga cctcggggcc gatacaagcc
      661 gttcacggcc tgttgactca ggccgatgag gtcgtcgtgg cgccgccggc cgacctgcgg
      721 gccggtgccc cggtgaccat ccggcggcgg acgttataag gctgcaccgt gtacgatccc
      781 gtcgtcacgc tccaacggat ctcgaaaacc tattcgcggg caggcacgga cgtgtccgtg
      841 ttgcgcgatc tctccttcac gattcccgcg ggaacgttcc tcgccatcat ggggccctcg
      901 gggtccggaa aaagtacgtt gctcaatgtg atggcgggga tcgatcggcc gaccagcggg
      961 tcggtcgtcg tggccggcac ccgactcgac gatctttccg aaggcgacat ggcgcgatgg
     1021 cgggcccggc acatcggcta cgtgttccaa tcgtacaatc tcattccggt gctcaccgcc
     1081 tcggagaatg tggaactgcc gctcctgctc acacatctgt cgcggcagga acggagggag
     1141 catgtgaaga cggccttgcg gctggtgggc ctgggggagc ggatggacca ttacccgcgc
     1201 cagctttcag gtgggcagga gcaacgcgtc ggtcttgccc gcgccatcgt cagcgacccc
     1261 accatgatcc tggcggacga gccgacgggc aacctcgatc gtgaatctgc cgaggatatt
     1321 ctgaccctgt tcggtcgatt gaatcgggaa ctgggaaaaa ccatcgtgat ggtcacgcac
     1381 gaccctcgcg ccgccgagcg tgcgcagatg attcgtcatc tggaaaaggg cgtcctggaa
     1441 tctccgccat gacgctcggc cggctcgtgc tccgcaatac gttgcgccgc ccggtccggc
     1501 tggccttgac ggtctggggg ctcgccatgg tcgtgctggc gttcggcctc atccgtctga
     1561 cgctcgacga atggcagggg gcggcaaaag cggccagcaa gaaccggctg atcacggtcc
     1621 atgcgatgtc gggggccttg ccgcttccgt tggcatacag agatcgcatt gcggtgctgc
     1681 cgggggtgcg gtccgtgcac tatggcgtcg agttcggcgg gatctacaag gatcccaagc
     1741 agccgttggc ctccttcgcc gagccggcgg cgacattgct gaccacatat ccggaaattc
     1801 tgctctccga gagggagcgt ctggcctttg tacaggatcg ccgcgggtgc atcgtcggcc
     1861 tgtccctggc cgagcggtac ggatggaaac tcggcgatgt cattccgctg accgggacca
     1921 actatccggg gcaatgggaa gtgattgtgc gtggcattta ccgcagcagc aacctcatga
     1981 tctggtcgga tcaggaattg tttctgcagt gggagtatct caatgaacag gtgaagacga
     2041 cacagccaga ccgcgccgat caggtgtcgt ggtacgcgat tgatgtgaaa gaggggagta
     2101 atccacgggc ggtggccctg gcgctcgatg cgctgtttgc gaactcgttt gccgagacac
     2161 gcacggaact ggagcaggcg ttcattgccg ggtgggtcga gcggtcgagc gcactgctgt
     2221 acggcctgga agcggtgtcc gcggtcatca acgggatcgc gctcctggtc ctggtgaacg
     2281 cgctggccat ggcgatccgg gaacggacca gggaatatgc cgtgatcaag acgctgggat
     2341 ttcagccacg gcatttttgc atcctggtgt tgggcgagtc gttgttgctg gccctctccg
     2401 gcggggccct gggattgggc ctgctcgtcc ccgcctcgcg gctctacgcg cagatgaccc
     2461 aatcgaacgg gtatgtggcc acttacgcgg tgtcgatgga gacggtgggg ttgtgtctgg
     2521 gcatgatggt gctggtggcc ttgggcgcgg cgatctggcc ggcggtccgt ctcatccgga
     2581 tgacgacgtt ggacgggctg cggcatgcgg gataggagcc tgtccgagta atccttcgta
     2641 gcgaggcgga ggagttgccg gcggatgcga ggccgcaggc tcgaaaaaac cggaaacgta
     2701 ttcgctgaat acgttgagga tttttacggg ccgagaacga cgcagatgtc ggcagatcat
     2761 ttgccgcagc agaatggcat tactcagaca ggctccgagg cggaggtagg aacatggtgc
     2821 ggtggggtct gtacagcctc agaaatctat gggctcgccg tgtgacgacg gcgttgaccg
     2881 tcgttggttt ggggctggtg gtgttcgtgt tgctggccgt gttgatgttg gcccatggat
     2941 tggagcggac actggggaag acgggcgatc cggagaacgt gatcattctg cgtaaaggcg
     3001 cgctctcgga aatcggcagt agcatgtccc gcgaggaaat acgcgcggtg gcagctcagc
     3061 cggatgttcg actgacggcg gatggccggc cgctggcggt cgctgaagtc ggactgcaat
     3121 tgacgctgcg gaaggggcag ggcggcagtc tgaccagcgt gaccttgcgg ggcacggcac
     3181 cagaggcgct ggcggtgcgg ccggcgatcc tgctgattca ggggcggctg tggaagccgg
     3241 gtaccaccga aatcgtcgtg ggtgcctcca tcgccaggca gtttcctgac gcccgtgtcg
     3301 atcgggtgtt gcacttcggg aatcgggcct ggaccgtggt cggggtgttc gaggccggcg
     3361 gcagcggatt cgactccgag gtctggggcg atgcggaaca attcctggcg acgtttcatc
     3421 gcaccacctt ttcgtctctg accctccggt tggcgcagcc tgcgacgctg cccgccttca
     3481 aggaccggat cgagcgggac ccacggttta aattctcggt gaagcgcgag ccggagtatt
     3541 acgaaggcaa ggcggagggc ctcgctagaa tgattcgcgt cacggggctg tttctgaccg
     3601 tggtcttcag tgtcggcgcg ctgctcggag cgacgatgac gatgtcggcg tcggtggcgc
     3661 agcggacgac cgagatcggg acgctgcgga ccatcgggtt cacgcggatg gatatcctgc
     3721 aggcctttct gctggagtcg atggcgctgg gcgcgatcgc gggcttgctg ggcgtcgccg
     3781 ccgcggtggg actccagtcg atgacgatct ccaccatgaa cttcgacacc gggaccgaac
     3841 tggtcttcgg ctttcacctc tcgggcgaga tgatcgcgat cgctctcgtc tttgcgatgc
     3901 tcatgagcat cgtcggcggc ctcattccgg ccacccgcgc cgcgcgtctc gtcatcgtgc
     3961 aggccttggg tgagcgaacg ttctgacgag gggattgggc ctcgcttgaa aatgttgccc
     4021 tcgatcgaca ggtgtttgaa caaggctcac attttgtagc acgcgtgagc atccgtcggt
     4081 agacaacttc tgcggaacgg cgtatttgat ggggcaggtg agaactatca gaccatgaac
     4141 cgacggacct ctcaaacctc agtcgccggc ctcgcactcg gaatcctctg ggtgtacatg
     4201 gtgctggccc tcgtggcgcc gggctgttcc ttcgcccatg ccaccccgtc ggatagtcat
     4261 catcagcatt caggcacgga atcccattcc accctctgct cctgggcctg tcaggcgaca
     4321 tcggatgtcg gcccgatttc ccagggtgat gtgggcacag cctgggtcgc tcaatcccac
     4381 acgttgatta cccctccgct ccttctgact tccggggacc tctcgtccct tcatgctcgt
     4441 gcgccgccca tccccgtgag gggatagttc gggcggggcg cgcgtcgcca ccgattcatc
     4501 gcacagagaa attcgatccc gtcgttcagg ccaagagcca ggcgggagtc ctgtcggtcc
     4561 tgtgctccgc cttgtccggg cgttcggccc gagctatttc actcaagcat gttgagtccg
     4621 aggatgggat tgccctccga tcccggaccg cccggcagcg caggcgcgtt tcctgtgacc
     4681 tccgctgccg gttcccacga cggaaccgtg aagtgctgaa tcggtcctga atggtggacc
     4741 cggttgtgcg ggaggctcgt gccccttcct gcagtcgggg gaggagcggt aacggaccgc
     4801 tcctatccac cgccaaggtg ttgaaggggt aggcgagatg gtggaacagc cgctgttgcg
     4861 tgtgaaagag gccgcgcaat tgctccaagt caataagtgg acgatctacc ggtggatcga
     4921 tgaaggacgg ctcaatgcca cgaagatcgg tggaggcagc ctccgcattt ttcgtgtctc
     4981 cgtgacggcg ctggtggaag gcaatcgaac ggatccgcgt ccccaagaac cgacgccgca
     5041 cctgaaggcg gtgccgctcg gtgggcgcag ggcgatgaaa cgataggtct gtcggaaacg
     5101 gatcctgggg ttgtgcaccc ttcttcagga tctgcctccc ggggtgagtg ggatggcctc
     5161 ccccttcacc ccggcccctc ggggccgacg gcaagaccgg gaggaaggag atgaacatga
     5221 tggtgagaga gatactaagc ctcattgtgg tgatgggggt ggcgtggaac ggcgggatcg
     5281 tgacgacggt tgctgaagcg ggggcggccc ccaaggacgg cgaaatcgcg ctcccggccg
     5341 attacaaaac ctggccgaag tttttgagcg aggtacagcg tgaggatgcc aagcaggtgc
     5401 gggaattgtt cgtgagccca gccggcgcca aggcttgtcc ggcctccgcg tttccgcagg
     5461 gcacgcaact ggttatggag ttgtacaagg ctaaagcgga aggcgaggcc ttggccaagg
     5521 gggcggacgg aaagctggtg aaaggcgacc tgctgaaggt gttcgtcatg gagaagaacg
     5581 aggggtgggg gcaggacgtc ccggacaatt tgaagaacgg cgcctgggtg ctcggggcct
     5641 atgggccgga cggcaaggcg ctggccgaag attttacgaa atgccgggcc tgccatgccc
     5701 cgctcgcgca gaaagatttc gtgcaccggt atgatgagtt ttgtgcgcag ggccataagg
     5761 cgcactagca ggatgcggaa aaagtccgcc agcggcgttc tcgcatcgct tcgcggctca
     5821 ccgtaccgca cgagtacgat tcgccccttc gctcgctgcg gccttgctgg acggcctttt
     5881 ttcgcatcct gcgggtgatg ctgataccga tttggaatag caggtcgttc atcatgacca
     5941 cgagcaggac agatggggcc tgtgtgcggg tcttgaccta cctcctcgca ggagtcccat
     6001 gggggagcgg tggttggccg ctccccgccc atcggccgct tgagtcacag gtccctcgga
     6061 tcctgaggtt gtgcaccctt cttcaggatc tgcctcccgg ggtgagtggg atggcctccc
     6121 cctccacccc ggcccttcgg ggctttccgg gctgttagtg cggagaggcc ggagacaggg
     6181 aatacaggac agagaatcga cggagtcggt ggagggagcg gcgcaccatg gctgattgca
     6241 cgaagaaaac cccgcggtgg ttactcatgg cattggtggt cggcatggct ttgtgcggcg
     6301 ttgatggggg ctgggctaat gagccggatg cttggaagca gttgtttact gatggtgtga
     6361 aggccaggga agcggcgcac tatgccgaag ctgagcagct cttgcagcgc gctctccgcg
     6421 aggctgagca ggccggtcca gacgacgccc gcgttgccac ggcagccaac aatctcggac
     6481 tgctgtatca cgaccagggt cgtctccagg aggcgcaatc gttatacacc cgtgcgctgg
     6541 ccatgtggga acaacacttg gggccggaac acgaagatgt cgcggcggcc ttaaacaacc
     6601 tggcggaaat cgcccatgcc gaaggcgatt ggggccgcgc ggagccgcta tatgaacggg
     6661 tgctggcaat cgagcgcaag gtgctgggcg cggcgcaccc cgatgtcgcg atcagcctga
     6721 acaatctggc cgagttgtac cgcaagcagg gacgggagac agcggccgaa tcgatgtatc
     6781 acctggccct gattctcatg gaagaggccc gtggcacgga tcaccccgat ctggggcccg
     6841 tgctcaacaa ccttgccgtc ttgtacaagg ggcgcggact ctatgcctgg gcggaaccgt
     6901 tctacctgcg cgcgctgaag atgcgccgcc tgcgattcgg cgatgaacat ccggcggtgg
     6961 cgatgagttt gaataacgcc gcctgcctct accaggccga aggactctac gcgtcggctc
     7021 aacgatattt cgatgaggca ctggccattg cggaacggac gtccggaccc caggcctcct
     7081 tgaccggcac gattgtcggc aatatggcgt ttctcgccga tgagcaggga ctatggaccc
     7141 aggcgcagtt gttgtatcag cgcgcgctcg tcgttcaaca ggcgcaactg ggcttgcatc
     7201 atcccaccgt cgggttgttg gtggagcggt atgcgcgcgt gctcgatact ctcgggcagc
     7261 cggttgaagc cggattgttc gctgcgcggg ccgcatcgat ccgggcgcgg gttcagccgg
     7321 gagtcgtgcg gcagggttcg gccatgcgcc aggggcctgc ggtcggagaa ctcacgcgat
     7381 aggtagtgaa ggcgtcagga agggaggtgc tccatgccgg ttagtgctgc ggtggcgtca
     7441 ccgcgtcggg cggagatggc tcagggcgct caacacaggg cggggttgag tgtggcagtc
     7501 gtgcgattgc tggcggtcgc cctcgatatc agcgagggct ggtccccggc gcctcccgct
     7561 gcgacgctct tgattccgtt cgggtatcgg gactggccga gcttgacctc tacggtggaa
     7621 tcgcccggcg ggcggaaacg gctccggttc tacgtgtgcc ccaaggcact gctcacgccg
     7681 gactatgaat cgtttcccgt ggggaccacg ttcgtcgtgg agtccgagcc ctatccggaa
     7741 ggccccggat cctatcgggc caggccgtcc gtgtttgcga tggagaagtg tgccggtctc
     7801 acgatggatg gcgcggggac gaggcagtac gaatcatgga tgtatgcgag ctgggattcg
     7861 cccgctcagc aggccatggc ggatcgtggc cgatgcggcg tctgtcgact gccctggctg
     7921 acgcccgctg acgttcgatg atcggtggac ggggctcgac ggttggactg cgctgaccac
     7981 gcagtgagct ccgatgaggg aagcgtacgc ccatgcgctt ccctctccca ccatcttctt
     8041 ctcaggcatg cgttgccctc acggtgagtg cggcctcctc tttccctact gaacaagaca
     8101 tgacaaatgc ggtagaatgc cgcccaatcg agtgctacat ccacttatca cacaggagga
     8161 attctgatga ctgtattgcg ttcaggcatg ctcgcgttcg ccgtgtccgc gctggtggcg
     8221 actgcgccgg tgggatacgc cctggccgac ggcggcgggc acgtgaagga aacgatcaag
     8281 cacgccaagg aaggcgtgga gcacgagaag gaagcgatca agcatctgga agagtcggtc
     8341 aaggccagca acgatccgca tgcgaaagaa gcgctggagc atgcgaagga agccgtcaaa
     8401 catgcggagg aatccctggc gcatgcggaa caggcttcga gccacaaagg gaaagcaaag
     8461 ggcaaataac gcgatctctg ttccaagggg gaggtgagtc ccatccggcg ttcccccttg
     8521 gaaccgtgcc gggggtcctt ccgcttgcgt gagttgacgt gttcggcttc gtgagacggg
     8581 tcaagcccag ccaagttctg gtcggctgcg gaagccacgt ttcaacgaca cgatcctgta
     8641 gcatcggtag gcatccgtaa gcgcgcatct gtagacggtt gagcgtgcag gggctataac
     8701 tcgactccct aggaggggtc ccccatgcga aaactcttcg tggccgtggt tcagatcctc
     8761 gtgctggccg gcgggtcagg agtgtgggcc gccgacgatt cactggaacg gttgttacgg
     8821 gcgcaggcgt tcaatcggga attcgaggga ttcgattcgt actacgtaaa gatcgaagcg
     8881 gatcagcccc agcctgacgg gtcgcatgaa gtgctggcgg tggccagcgg aaaattttca
     8941 gacaatacga agcgtatgaa agtgctgttt ctcatcgtcg atggtcacat tgtcggtggc
     9001 caggtgttgg aaggcaccgg tcttcctccg tgcctggcac aggagcatcg tccgtcctca
     9061 tctctttaac ctgacaggag gttctatgtt gagcaggatt gcggtccttt tcatgggcgt
     9121 gttgggcgtg ttggcgagcg gaacgtctgc gttggcgctg caggccggcg gtgtcgaagt
     9181 cggtgacctc gaaaccggtt ccgtcgtcgg tcagccgttt aaggaattgg tcgtggatgc
     9241 ggaattatat gccgttgaga tcggcaacat gaaggcgtgg tatccgccga ccacggtcat
     9301 cgatttcaaa gtccggccgg gtcgtccgct gttgatcaag gtcacgaata ccaccgcaac
     9361 ggagcgtggt tttcaaatga tcgatacagt caatgcagcg tcgccgttcg tcttaaaggc
     9421 cgaggtccgg ttgaagcccg gtgagacgaa gtacatcggc gtgccgacca gcgatttgtt
     9481 caatcaaacc cagggcaata cgcttgagta ccgcgaccag ctcaatccca agcagcccgg
     9541 cggcaaactg ctgatgatca agtaacagac cctgtctgta gtgcgagtac acacgccccg
     9601 ttcatccgca gggatggacg gggcgttgtc gtttcttcac cgttgtcgaa acgatgatcg
     9661 acagacggcc gggtttacca atagtaggga aagcgcccgt agggtcccca gtagggatgc
     9721 cagaacggac tgtaataggg gtagggacgg atacgggaca tagtcgagtc ttgcgatgga
     9781 atccaggtgc ggaacgattt gatgtcgatg acgggatagg tatagtcggt ctcgtcgagc
     9841 ggcaaggtca tcgagccggt gagctcaccg gtgatggtca ggaacgttcc ctggggaatg
     9901 gttgccggat ccagaaagtc ccgttggacc gcgacaaagc gcccctgaga tttcatcaga
     9961 tcgagcgtcg gttgttgcga gtcgttgagc ggtaactgca acacttcgat ccgcgtgccg
    10021 tctttcatgc gtcgcgcggc gagcacttgc ccgccgagca cgagggtctg ccctttgaac
    10081 gagtccggtg cggccttgat ttgagaaaac ggcggaacgt gcgggccgcc gtcggccgac
    10141 tcgtgcatgt cgccggacga cgcgcaggcc gccaggatcg cggcgaacca caaaatgccg
    10201 atcccctgta gaagtttcac tcgcatcgtt ggcattatat cagacgcata agtggacgag
    10261 cattgtttat aatgccgatg agcctgttga accgaaccaa gtttgaaaaa ggagtgcagc
    10321 gtatggcgaa gcgtcgtgga tggcggggca gtctgttgat gggcctgatg gcggtggcgg
    10381 ctctcgggtt cgtgggaacc gcgcaggcgg ggaaggcgga actcaagggc aactttcagg
    10441 tgctgagcga cgagaagtcg acgcacaagc cggggaaagt gcaactgact gaattcgcag
    10501 atttctactg cccgcattgt caccggttcg acggcgaggg gctggcgatt ctggagaagg
    10561 aattcggcca taagctggaa gcggtcatgg tggggtatcc cgtgattccc ggcaagttgc
    10621 cgaccgcctt cgatatgtat gaacaggcca agacgatggg gaagggcgct gagatgaagc
    10681 gcgcgttgtt ccggaccatc cataaggaca agatcggcat catcgacaag gcgatccgcg
    10741 aagtgttgat tcgtgaagtc gggctcgacc cggcggcgtt cgaagaaggg ttggccagcg
    10801 ccaagccggc gaaggccttc gaagacggac ggaaatgggg cgatcgcatc aagattcaac
    10861 agacgcccac cgttctgctc gacggcaaca tcaaggttga gcaaatcgat ccggagaatt
    10921 tgaagctgat cattcacagc attctggacg gagacggcaa gaagtaaggg tcgtatctcg
    10981 tgaagtgtga agcgtccctc gaccggaacg agatacgaac gacgcgagat gaggtgcggg
    11041 ggtatggcga ctgatccgat ctgcggcatg acggtggaac cggccacggc ggcagggcat
    11101 ttcgattatg acggccggac ctactatttt tgcagtaccc attgcctcga tcggtttcgt
    11161 gccacgccga atcagtatgt gaagacagcg tctgttgcga ccgccgccat gcccgctgcc
    11221 ggtcggcgtt cgctgccgat gatgcaggcg atgcctgagc aacagccggt tgcgggcggc
    11281 gaacgggatc ccgtttgcgg gatgaccgtt cagcctgcga ccgccgccgg gtctcatgcg
    11341 catgcgggca agacgtacta cttctgttgt cagggatgtc tcgaaaagtt ccgggccgat
    11401 ccggtccggt acttgtcgcc ggtgcccgct gtgtcgccaa cagcgtcacg gcagttcggg
    11461 ggaaagtcgc tcccgatgct gggctcggcg acgccggata aagcggtcac aggcgtcatc
    11521 gatccggtct gtggcatgac ggtggaaccg gccacggcgg ccgggtcgtt cgactaccag
    11581 ggcacgacct actcgttctg ttgccagggc tgcctcacca agtttcgcgc cgatccgcag
    11641 cggtatctcg cccccgcatc tgctcagtcg actgcgcctg ctgccccgcc tcctcccggg
    11701 acgaaatacg tctgtcccat gtgccctgag gtgttggaag aaaagccggt gccctgtccg
    11761 aaatgcggga tggcactgga gccggattcg attcagccgc tgccgaccaa aacggaatat
    11821 gtctgcccga tgcatcccga ggtggtgaag gcggagcagg gggcctgtcc gatctgtggg
    11881 atggcgctgg agccacgaac cgtcacggtc gaggaagagc tgaatcctga gttggtggac
    11941 atggcccgac ggttctgggt gggactgggt ccggcggcgg cggtcttcgt gctggccatg
    12001 tcgcctatga tccccggtca tccgattcag catcttgtga ccgatatgca atcggcctgg
    12061 gtgcagttcc tgctgagcac accggtggtc ctctgggccg ggtggccgtt ctttcagcgc
    12121 ggctgggctt cg
//
LOCUS       DAORRQ010000092        11773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_260_length_11773_cov_99.253798, whole genome shotgun sequence.
ACCESSION   DAORRQ010000092 DAORRQ010000000
VERSION     DAORRQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_11773_cov_99.253798"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="PK224_18675"
     CDS             complement(<1..1973)
                     /locus_tag="PK224_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB17829.1"
                     /translation="MLSAPPQSPAPLGVSGRLAALFVGSKLTPLIIIASLLLGLFAIV
                     LTPREEEPQIVVPMADVWLSFPGASAKVVEEQLTKPIERKLSEIKTVEYVYSISRPGG
                     ALIIVRFYVGEPMEQSLVDLYDKLMSNQDLLPPGAQPFQVKPRDVNDVPIVTLTLSSE
                     RYTDFQLRQLGDQVLEDAKKVPGTAAGFVVGGRGRELRVQIDPSRLKAYGLTPLRVAG
                     VIQGENLALPTGRFESRNESFLVETGRFIRSKEDLEGVVVGVNEQRPVYLRQVAEVTD
                     GPTEATSYVWHGEGPGGAHETLHVKGDTNTPASHLLPFTSHENQAVTVAIAKQAGTNA
                     VTIAQGVIRKVEELKGTRIPDEVRVTVTRDYGETADEKANELLWHLLIAVVAVVVFLG
                     VTLGLRPALVVSIAIPLTLALTLFISMLIGYSINRVTLFALIFSIGILVDDAIVVVEN
                     TYRHLTLRIRPHREASLFAVDEVGNPTILATLTVIAALLPMAFVSGLMGPYMRPIPVN
                     ASIAMFVSLLVAFIVIPWFCQTCYRPGVHMAGVDHESFEGSRSYRLYQRLLAPVLSRP
                     VVAYLVLAVIALLLVGSVALFYTRAVVVKMLPFDNKSELQLVIDMPEGTTLEETARVT
                     KALTQYVRTIPETRDYQAYVGTASPFNFNGLVR"
     gene            complement(2071..3159)
                     /locus_tag="PK224_18680"
     CDS             complement(2071..3159)
                     /locus_tag="PK224_18680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB17830.1"
                     /translation="MFRNNDASVKAGWLVVALLLAAGCGQSEQPPRGGEAPSVTAVQP
                     ALQLAVIEVKTGQVPVTVEVTGQVTAVYQATLASRIQGTIDNLLVREGSIVRKGQTLM
                     VLDNRDLQAELSRVTAELDNANAHQTRMEDLYRKDAVSKQELENATRGLRVAEAARKA
                     VLAQLSYTIVKAPFDGVITEKKVEIGELASPGQPLLKMEDPEQLRLEATVAESDVRVV
                     SVGSSVPVVIDALGADPLKGKVSQILPAGDPQTHTFTMKVELPRTGGLKSGMFGRLRL
                     EKGMNTTLLLPKSAFIERGELASLFVVGSDRVARLRWVKIGRTLEHGVEVLSGVDAGE
                     RVIVEGAKGRDGALVQDMTAVASPPQGS"
     gene            complement(3146..3334)
                     /locus_tag="PK224_18685"
     CDS             complement(3146..3334)
                     /locus_tag="PK224_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRB17831.1"
                     /translation="MRLNESLRLIAGVFVLGAVVLGATVHPYYNYFAAFVAANLIQSS
                     FSGWCPMMALLRRLGVQE"
     gene            complement(3495..4520)
                     /locus_tag="PK224_18690"
     CDS             complement(3495..4520)
                     /locus_tag="PK224_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17832.1"
                     /translation="MMRYPHSLLSTLCRAALWGVLASLMWGPDRPAQAEWSAVAEQKT
                     SYTSDAFQFSSARRLRLTEDPSQPTVVSADKPEDVIWEPALEVMHSTPTAQGKNELSV
                     KAQGAIYTNNPIFNHADYRIQDRFSLDQATSVMVRYRYVPNLFLGPNFERRTGTRSIQ
                     EERVSSHHWRAEIERRLTDTVTGTLITRYGLRRYNDSFAERNTNFFTLGPRVDYRAME
                     WLTVTLTYLYERGLADGHKDTQFADDVSYYLHMVSAGTEFRLNRQWDLGFTYIHRRKT
                     FTSGLAGDTHVGRFDATHQGIAELRYHMSAAATVMLSFQYGKRTSTNALRDFQDSILS
                     IGGQYRF"
     gene            complement(4656..5453)
                     /locus_tag="PK224_18695"
     CDS             complement(4656..5453)
                     /locus_tag="PK224_18695"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRB17833.1"
                     /translation="MIDQALRSMLAGVPIGLQLGATGTGGAILGLPLMVYLAGIPMQQ
                     AAAMSLIIVATSSLLGAWEYGRQGQVKATAALAFSWTGAIGSWAGAFGHRLVREEILL
                     VLFGLLLLLVRGAMTRYRQMPSGPDEEESCATRFPRTCWVKVGGIGLAVGTMNGLFGV
                     GGGFMVVPALILILGFPARLAIGTSLSIIACISIGGVVGHLQFGRINWPLTAWVLAGS
                     LAGMLLGVRVGTWLSPTTMGRITAFITVSIAVSMIVVNLVKLWGSQI"
     gene            complement(5680..6927)
                     /locus_tag="PK224_18700"
     CDS             complement(5680..6927)
                     /locus_tag="PK224_18700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRB17834.1"
                     /translation="MARIVIIGASIGGLPAAYEARELLAKKHKVTVISNVESFHFVPS
                     NPWVAVGWRTRKDISFALGPVLEKKGVEYIHAMAERIEPEQNRVITSKGEVPYDYLII
                     ATGPKLNFGAVPGLGPSGYTQSVCNVDHAEQAWGTYQAFLKDPGPIVVGAAQGASCFG
                     PAYEMAFILDADLRKKKLRKKVPIYFVTPEPYIGHMGLAGVGASRRLMEDEFADHSMK
                     PICNTAIKEVQPGKLLLDGGQEIPFRYSMIIPPFAGVDAVAGTPGLCNPKGFVNIDAY
                     QANPKYKNIYSVGVCVAIPPVEQTPIPTGAPKTGYMIESMVSAAVHNIKADIEDDPKR
                     ETATWNAICLADMGDTGVAFVALPQMAPRNVTWAKKGKWVHLAKIALEKYFLRKMKKG
                     VSEPYYEKVILKALGIAKLEGPG"
     gene            7283..7963
                     /locus_tag="PK224_18705"
     CDS             7283..7963
                     /locus_tag="PK224_18705"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HRB17835.1"
                     /translation="MLHRLFAGKGLIIALAVGFIAVESIVAASFLSLLHFKTSNNWAT
                     KSEQVLLELERMMSAVAGAETSQRGYIITGSDEYLAPYREAVVTLEDQIRRIGGLTRD
                     NSIQQDRVAYLVTQVEQRSEEMDQAIVTRRSKGLPYAKSVVAVNQQNRTMDTIQDISA
                     QIRKEETRVLERHRADSEAWAFTTGSFAAVFFLLNAVVFTLCGIVIKMALTSQAQAER
                     LIHSLQRH"
     gene            complement(8671..9144)
                     /locus_tag="PK224_18710"
     CDS             complement(8671..9144)
                     /locus_tag="PK224_18710"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRB17836.1"
                     /translation="MFALVWIHLLAAMVWIGGMVFLSVVLVPVLKRDGAFAQYALLFR
                     TVAYRFRAVVWGAMGILVATGLTLAAGRSIPLMDPSRWPTIFAAKIGLVALLFTLTLL
                     HDLVVGPRVRRSLGIPEAERSARDRVLLRYSVLVPRISLLVALLVLLFAVVLART"
     gene            complement(9409..9906)
                     /locus_tag="PK224_18715"
     CDS             complement(9409..9906)
                     /locus_tag="PK224_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17837.1"
                     /translation="MKIAVHLPDNGALPHLLGEEAWRHTFTRRIDVHGAARSHVVTFA
                     SLPEHIDVALQVIGESVRIQGAWATVNDQRMSSLVALWNRLDCYRQSLAVADARIFCA
                     SKAASLQAMSGCEAVLCMSPCQFLCRQCAQIGTHPVTGLVHPEAQLLAMLGEIGWCPN
                     LRRPD"
     gene            complement(10061..10762)
                     /locus_tag="PK224_18720"
     CDS             complement(10061..10762)
                     /locus_tag="PK224_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17838.1"
                     /translation="MMRVRRVVRNIGMWGAGLAIAAVFGVACVQPASAEEGTGNMVFF
                     KGGFMNLNQDRSGQIFTDTAGIAGANGGNAGWYAGAGLDLVMSKDVWGGMDKTWVVGE
                     IGLQFNSINSNRVNNAAGSIATRTLTDTLATDPQKVQLTMVTIDVAPKIKFMEGSAFR
                     PWVIPIGLDIHVISPPSNRTQYLDLGVQFGGGFEYAVWKAFKVGVDARYHLTARLTNT
                     NNDYFQVGPYVGISF"
     gene            complement(11001..11549)
                     /locus_tag="PK224_18725"
     CDS             complement(11001..11549)
                     /locus_tag="PK224_18725"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRB17839.1"
                     /translation="MVSQSSRRTSLTVDPLALLKREHRMILDRLAMVETAMSPRSSGS
                     GAVRGTNRETLRELLEFFTGPVDVHFKREAMLMGDLRRILGRKQEEQEQFQSFLDEHR
                     ALKADAAAVMRQLVKKRTDGQDAAASKAFGGLRTLTGELHALIRRYRGQIACEERLLF
                     ALAEMRLSAEQRRRISRRMLQV"
BASE COUNT         2461 a         3755 c         3363 g         2194 t
ORIGIN      
        1 tgccgcacca gtccgttgaa attgaacggc gaggccgtgc cgacataggc ctggtaatcg
       61 cgggtttccg gaatcgttcg cacgtactgc gtcaaagcct tcgtcacccg ggctgtttcc
      121 tccagcgtcg tgccttccgg catatcgatc accagctgca attcgctctt gttgtcgaac
      181 ggcagcatct tcaccaccac ggcccgggta tagaacagcg caaccgaccc gaccagcaag
      241 agcgcaatga ccgccaacac caggtaggcc acgaccggac gggacaacac cggagccagg
      301 agccgctggt acaggcgata actgcggctg ccctcgaacg attcgtgatc gaccccggcc
      361 atgtgcacac cgggccggta acaggtctgg cagaaccagg gaatgacgat gaacgcgacc
      421 agcagggaca cgaacatggc aatcgacgca ttcaccggga tgggccgcat gtagggcccc
      481 atcaagcccg agacaaacgc catgggcagg agcgcggcaa tcaccgtcag ggtggccaga
      541 atcgtcgggt tgccgacctc gtccacggca aacagcgagg cttcgcggtg cgggcgaatc
      601 cgcaacgtga gatgccggta ggtgttctcc accaccacga tggcgtcatc cacgagaatg
      661 ccgatggaaa agatcaacgc aaacagcgtc acgcggttga tgctgtagcc gatgagcatg
      721 gaaatgaaca gggtcagggc gagcgtgagt ggaatggcga ttgagaccac cagtgcgggg
      781 cgcagcccca gtgtcactcc gagaaacacc acgacggcga ccacggcgat caggagatgc
      841 cacagcagct cgttggcttt ctcatcggcg gtctcgccgt aatcccgcgt caccgtcacc
      901 cgcacctcgt cgggaatccg ggttcctttg agttcctcga cttttcgaat gacgccctgc
      961 gcaatcgtca cggcgttggt gccggcctgt ttagcaatcg ccacggtcac agcctgattt
     1021 tcgtgagacg tgaaaggtaa caggtgagac gcgggagtat tcgtgtcacc tttcacgtgt
     1081 aacgtctcat gcgcaccgcc gggtccctcg ccatgccata cataactggt cgcttcggtc
     1141 gggccatcgg tcacctcggc cacctggcga aggtacaccg gccgttgttc gttgacgccg
     1201 acgaccactc cctccaggtc ctctttcgag cggatgaacc ggccggtctc caccagaaaa
     1261 ctttcattcc ggctttcgaa ccggccggtg ggcaacgcga gattttcgcc ctgaatcacg
     1321 ccggcgaccc gcaacggcgt caggccatag gccttcaggc gcgatggatc gatctgcaca
     1381 cgcaactcgc ggccgcgacc gccgaccacg aagccagctg cagtgccggg gaccttcttc
     1441 gcatcttcca agacctgatc gcccagttgc cgtagctgga aatccgtata ccgttcactg
     1501 gacagggtca gggtgacgat cggcacatcg ttcacatccc tcggtttgac ttgaaacggc
     1561 tgagcgccgg gcggaagcag gtcctggttc gacatcaatt tgtcgtagag atccaccaga
     1621 ctctgttcca tcggctcgcc gacataaaag cggacgatga tcaacgcccc gcccggacgg
     1681 gagatggagt agacatattc gacggtcttg atttcggaca gcttgcgctc gatgggcttg
     1741 gtgagctgct cttcgactac cttcgcggag gccccgggaa acgagagcca gacgtcggcc
     1801 atgggcacca cgatctgcgg ctcttcttcg cgcggggtca ggacaatggc aaacagcccg
     1861 agcaggaggc tggcgatgat gatcaatggg gtgagtttgc tgccgacgaa gagcgccgcg
     1921 aggcggccgc ttactccaag gggagctggg gactgtgggg gagctgatag catatggctt
     1981 atagcgtatg gatggggttt gacgccattc gacagatcag cctgttttcg atcctgccat
     2041 acgccatgag ctataggctc ttttggcgag tcacgatccc tgaggcggcg atgccactgc
     2101 cgtcatatcc tgtaccaacg cgccgtccct gcccttggcc ccctccacga tgactcgctc
     2161 accggcgtcc acgccggaga ggacttcaac cccgtgctcc agcgtacggc cgatcttgac
     2221 ccatcgaaga cgagccaccc ggtcgctgcc gaccacgaac aggctggcta gctcaccccg
     2281 ctcgatgaag gcggacttcg ggaggagcag cgtcgtattc ataccttttt ccaggcggag
     2341 ccgaccgaac attcccgact tgagcccgcc cgtcctggga agctccacct tcatcgtgaa
     2401 ggtatgagtt tgcgggtcgc cggcaggaag aatctgcgac accttcccct tgaggggatc
     2461 ggcgcccaac gcatcgatca cgaccgggac cgacgacccg accgacacaa cccgcacatc
     2521 gctttcggcc acggtcgctt ccagccggag ttgttcggga tcttccatct tgagcagcgg
     2581 ctgcccgggg gaggccagtt ctccgatctc aaccttcttc tccgtgatca caccatcgaa
     2641 cggcgctttc acgatcgtgt agctcaactg cgccaggaca gccttgcgag cggcttcggc
     2701 cacccgcagc ccgcgcgtgg cattctccaa ctcctgcttg gacaccgcat ccttccggta
     2761 gaggtcttcc atccgggtct ggtgcgcgtt ggcattgtcg agctcagccg tcacccggga
     2821 taactcggcc tgcaagtcac gattgtcgag caccatcagg gtctgcccct tgcgtacgat
     2881 gctgccttcg cggaccagca gattgtcgat ggtaccctgt atgcggctgg ccaacgtggc
     2941 ttggtagacc gccgtcacct gcccggtcac ttcgaccgtc accgggacct ggccggtctt
     3001 cacctcgatc accgccagct ggagggcggg ctgaacggcc gtcaccgacg gagcttcccc
     3061 gcctcgaggc ggctgctcag actgcccgca gcccgccgcg agcaggagcg cgaccaccag
     3121 ccatcccgcc ttcacagatg cgtcgttatt cctgaacacc cagcctccgc aagagagcca
     3181 tcatcgggca ccagccgctg aatgacgact ggatcagatt ggccgcgacg aacgcggcga
     3241 agtaattata gtagggatgg actgtggcgc ccagcacgac cgcgcccagg acaaacaccc
     3301 cggcgatcaa acgaagcgat tcattgagtc tcatgtgccg cacccccttt cttacaccat
     3361 atgagtaagc agggggaaaa aggattcgct agaaggagaa gtccgccatg aatcaaggac
     3421 ttccctcgtc tccgacacac gggctcttct tcccccggca ccaacttggg cgccgccgcc
     3481 ccgtacgcga acgatcagaa gcgatattgg ccgccgatag aaagaatcga gtcctgaaag
     3541 tctcggagag cattcgtcga ggtacgtttg ccgtattgaa aggacagcat caccgtcgcg
     3601 gcagccgaca tgtgataccg cagctccgcg atgccctggt gcgtggcatc gaaccggccc
     3661 acgtgcgtgt cgccggcgag gccgctggta aatgtttttc gccggtgaat ataggtgaac
     3721 ccgagatccc attgtcgatt aagccgaaac tccgtaccgg ccgacaccat atggaggtag
     3781 taggacacgt cgtccgcaaa ctgcgtgtcc ttgtgcccgt cggccaaccc ccgttcatag
     3841 agataggtca gcgtcactgt gagccactcc atggcacggt agtcgacgcg agggccaaga
     3901 gtaaaaaaat tcgtattccg ttccgcaaat gagtcgttgt aacgcctgag cccgtaccgg
     3961 gtaatcagcg tgccggtgac ggtgtccgtc aaacggcgtt cgatttcggc gcgccaatgg
     4021 tgcgagctga cccgctcttc ctgaatcgag cgggtgccgg tgcgccgctc gaaattgggt
     4081 cccaggaaaa gattgggcac ataccgatac cgcaccatca ccgaggtcgc ctggtcgaga
     4141 gaaaatcggt cctggatgcg gtagtcggca tggttaaaga tggggttatt ggtgtagatc
     4201 gccccctggg ccttcaccga gagttcattc ttcccctgcg ccgtgggcgt tgagtgcatc
     4261 acctccagcg ccggctccca aatcacatcc tccggcttgt cggcggagac caccgtcggt
     4321 tgcgaaggat cttccgtcag gcggagccga cgcgcggaag aaaactgaaa ggcatcggag
     4381 gtatagcttg tcttctgttc agcgacggcc gaccattcgg cttgcgccgg ccgatccggc
     4441 ccccacatca aacttgccag cacgccccac aaggcggcgc ggcacagggt ggacaggagg
     4501 gaatgcgggt accgcatcat cgattcgtgt ggcccacagg agcatggaat ggggaacaca
     4561 tccccgtcag tttcgcacgc catcgcggaa caggggccat cccacgagag cctgctgctg
     4621 agcgtccgac agaacaagcc tcccgtcaac aaagttcata tttgtgaacc ccataacttc
     4681 accagattca ccacgatcat gctgaccgcg atcgagaccg tgatgaaggc cgtaatgcga
     4741 cccatcgtgg tcggagaaag ccaggtgccg acccgcaccc ccagaagcat ccccgccaga
     4801 ctccctgcca atacccatgc ggtgagcggc cagttgatcc gcccgaattg gagatggccg
     4861 accaccccgc cgatggagat acaggcaatg atgctgagcg acgttccgat cgccaacctg
     4921 gccgggaacc cgaggatgag gatcagggcc gggaccacca tgaatccgcc accgactccg
     4981 aacaaaccgt tcatggttcc caccgccagt ccgatgccgc cgaccttcac ccaacaggtg
     5041 cggggaaagc gggtcgcaca actttcttcc tcgtcagggc cggacggcat ctggcgatag
     5101 cgagtcatcg cccctctgac cagcagcagc aggagcccga acagcaccag gaggatttcc
     5161 tcgcgaacca ggcgatgtcc gaatgcgccc gcccacgaac cgatcgcgcc ggtccaactg
     5221 aacgccagag cggctgtggc cttgacctgt ccctgccgcc cgtattccca ggcgcccagc
     5281 agcgacgagg tggccacgat gatcaacgac atcgccgccg cctgctgcat gggaatcccg
     5341 gcgaggtaga ccatgagggg gaggcccaga atcgcgccac ccgtccccgt cgcgccgagt
     5401 tgcaagccga tggggacgcc cgccagcatg gaacgcaacg cctggtcgat catgccggtt
     5461 ccttctcgat cgtcgccacg cgatgccccg tccccatgtc gtttagcccg gattattcat
     5521 gaatgtcgtc gcgaggcgtt cgagaccgaa gcccgccggg gcttctcgca agcgaggccg
     5581 acgcaggcgt actgacagtc cgtcgaggag gccgaacgag aacagccccg ccgggcgaga
     5641 gaatcggacg acggagcagg cattcatgaa tagtccgggt tagcccgggc cttccaactt
     5701 ggcaatcccc agggccttca aaatcacctt ctcatagtag ggctcgctga cgcccttttt
     5761 catcttgcgc aagaaatact tctccagcgc aattttggca aggtgcaccc acttaccctt
     5821 cttcgcccag gtgacattcc gcggcgccat ttgcggcaac gccacaaagg ccacgcccgt
     5881 gtcccccatg tcggcgagac agatcgcatt ccaagtcgcg gtctcgcgct tcgggtcatc
     5941 ctcgatatcg gccttgatgt tgtgcaccgc cgccgacacc atcgactcga tcatgtaccc
     6001 ggtcttgggc gcaccggtcg gaatcggagt ctgctccacc ggcggaatgg ccacacagac
     6061 gccgaccgaa tagatattct tatacttggg attggcttga taggcatcga tattcacaaa
     6121 ccctttgggg ttgcagagcc ccggcgtccc tgccaccgca tccacgccgg cgaacggcgg
     6181 aatgatcatc gagtagcgga acgggatctc ctgccctccg tcgagcaaga gtttgcccgg
     6241 ctgcacttct ttgatcgccg tattacagat gggcttcatc gaatgatcgg caaactcatc
     6301 ttccatcaaa cggcgggagg cgcccacacc tgcgagcccc atatgtccga tatagggttc
     6361 cggcgtcaca aaatagatcg gcaccttctt ccggagtttc ttcttgcgga gatcggcatc
     6421 cagaatgaac gccatttcgt aggccggtcc gaaacaggag gcgccttgcg ccgcccctac
     6481 cacgatggga ccggggtctt tcaggaacgc ttggtaggtg ccccaggcct gctcggcatg
     6541 gtccacattg cagaccgatt gcgtataccc gcttggcccg agtccgggca ccgcgccgaa
     6601 gttcagcttc ggcccggtgg cgataatcag gtagtcatag gggacttcgc ccttcgaggt
     6661 gatgacacga ttctgttcag gctcaatccg ctccgccatg gcatgaatat attccacgcc
     6721 ttttttctcc aacacagggc ccagggcaaa actgatgtct ttcctcgttc gccatccgac
     6781 cgccacccag ggattggagg ggacgaagtg gaacgactcc acgttggaga tcaccgtcac
     6841 tttgtgtttc ttcgccagca attcccgggc ttcataggct gccggcaacc cgccgatgga
     6901 cgcaccgatg atgacgatcc ttgccatgat ccacctcccc tcaaagtaga ttcctaccgt
     6961 ccttgcctcg ctgcctgatc ccggccctcc cgcgagtcac ccgttctgct ccaaacctgg
     7021 gactatgcac ggtccgttcc cactcctctt gttaagagcc atcacaggtc atttggattc
     7081 agccggaata gagataaacc ggctgcgggg aaattttatg ccccgctgca agactggggt
     7141 cggggtaggg atccctcatt ccactgtgtc tcgaacgaac actacgacag atcttacgat
     7201 acctaggggg atttgtcggg ttgacatagc cactgactgg ccggtactcc tactatggac
     7261 gtattatcga ggagggttcc ccatgctcca tcgtcttttt gccggcaaag gccttatcat
     7321 cgcccttgcc gtgggattta tagcggttga atccatcgtc gcagcgagtt tcctgagcct
     7381 gctgcacttc aagacttcca acaattgggc cacgaaaagc gagcaggtgc tgctcgagtt
     7441 ggaacggatg atgagtgccg tggcgggggc tgaaaccagc caacgcggat acatcattac
     7501 cggctcggac gagtaccttg caccctatcg cgaggcagtc gtcacgcttg aggaccagat
     7561 ccggcggatc ggaggactga cgagggacaa ctcgatccag caggaccggg tggcctatct
     7621 tgtcacacag gtcgaacagc gatcggagga aatggaccag gccatcgtga cacgccgcag
     7681 taaggggctc ccctatgcca agtcggtcgt ggcggtgaat caacagaacc ggacgatgga
     7741 taccatccag gatatcagcg cgcaaatccg gaaggaggaa accagggtct tggagcgcca
     7801 ccgggcggac tccgaagcct gggcctttac gaccggctcg ttcgccgccg tgttcttcct
     7861 cctgaatgcg gtggtgttca ctctctgcgg catcgtgatc aaaatggctc tcaccagcca
     7921 ggcacaggct gaacgcctca tccactcgct ccaacgccac tagcccggat attcataaat
     7981 gccgtcgcga ggcgtccgag acggaagccc gccgaggctg ctcgcacgta aggtcgacgc
     8041 aggcgtactg acagtacgtc gaggaggccg aacgagaaaa gcctcgccgg gtgagaggat
     8101 cggacgccgg agcaggtatt catgaatgat ccgggctaga ctgctccggc aacacgatag
     8161 gacctgcgaa gcagcagcgc gacacactca acaggcggcc gcgttgctgc ccgctcacag
     8221 acgccgtccg cgcttccacg agagacaaac gacgcttcac caacgacgaa gctattcctt
     8281 gtgatcttcc tgctaggaat ctgtccgagt catccttcgt tgcgaggcga aggagctgct
     8341 gggagatgcg aggccgcagg ctcgaaaaaa ccggaagcgt attcgctgaa atacgttgag
     8401 gattttttcg ggccgagaac gaggcagatg gctgcagatc atttgccgca ggagaatggc
     8461 atgactcgga caaacttcta gcccgcatta ttcatgacgg ttcgtcgcga aagtgcggag
     8521 tcgcacagcg agacgggcgc gcggaggcgt gagggaggaa ggcatccttg aacaggatgt
     8581 cgaccgaccg agcggcgagc ccgcccgccg acaggctagt cgcagatgcg aatccgcgat
     8641 tgcagcagaa acgctcatga ataatgcggg ttaggtgcgg gccaggacga cggcaaacaa
     8701 cagcaccagc agcgcgacca gcaatgaaat acgcggaacg agcaccgagt aacgcaggag
     8761 cacccgatct ctcgcggatc gctctgcttc agggattccc aagctccgtc gcacacgcgg
     8821 tccgaccacg agatcgtgca gcagcgtcag ggtgaacagc agcgccacca atccgatctt
     8881 cgcggcaaaa atcgtgggcc agcgggaggg gtccatcagc ggaatcgatc gaccggccgc
     8941 tagcgtcagt ccggtggcca cgaggattcc catcgccccc cacaccacgg ctcgaaaccg
     9001 ataggccacc gttcggaaca gcagggcata ctgagcaaat gccccgtccc gcttcaacac
     9061 cggcaccagc acgacggata ggaataccat ccccccgatc caaaccatgg ccgccagcaa
     9121 gtgaatccag accaatgcaa acacaagacc atcctttcgc tctcatcgat gggcgtacta
     9181 tgaccgagga gggacggaac ggcaagggag gggcggcata gagcaggagt ctgcccgagt
     9241 cattcttcgt tacgaggcga aggagctgct gggagatgcg aggctgcagg cgcgaaaaaa
     9301 ccggaggcgt attcgctgga atacgttgag gatttttttg caccgagaac gacgcagatg
     9361 ccggcagctc gtttgccgca gcagaatggc atggctcaga acacactcct agtcgggacg
     9421 acgcagattc gggcaccatc cgatttcgcc caacatggcc aacagctggg cctccggatg
     9481 gacgagcccg gtaacagggt gtgtcccgat ctgcgcgcat tggcgacaga gaaactggca
     9541 gggcgacatg cataaaaccg cctcgcaccc ggacatggcc tgaagactag ccgccttgct
     9601 tgcgcaaaaa atccgtgcat cggcaacggc gagactctgc cggtaacagt ccaaccggtt
     9661 ccacagggcc accaagctgg acatccgttg gtcgttcacc gtcgcccagg ctccctgaat
     9721 ccgaacggac tctccgatca cttgcagggc gacatcaatg tgctcaggca ggctcgcaaa
     9781 ggtcacaacg tgtgagcgcg cggccccatg cacgtcgatt ctgcgagtga acgtatgccg
     9841 ccacgcctct tcgcccagca ggtgcggcaa ggcaccgttg tccggaagat gtaccgcgat
     9901 cttcaccaag gctcgacctc atgaatcctg catgcaggac cgctgccatg ccggcccgac
     9961 ctctcggcac cgaccggcat ggcagcgtcg tactcctctt gcctacagtc gaattcgcga
    10021 tcttgaaacc gggcgtctta ctgaccgcct ttatttcctc ctagaacgaa atcccgacat
    10081 acggcccgac ctggaagtaa tcgttgttgg tgttggtcag tcgcgccgtc aggtgatagc
    10141 gggcgtcgac gccgacctta aaggccttcc acaccgcata ttcaaatcca ccaccgaact
    10201 gtaccccgag atcgagatac tgtgtccgat tcgacggagg gctgatcaca tggatgtcca
    10261 gaccgatcgg aatgacccag ggcctgaacg cgctaccctc catgaatttg atcttcggtg
    10321 ccacgtcgat cgtcaccatc gtgagctgca ccttctgcgg gtcggtggcc agggtgtcgg
    10381 tcaacgtccg ggtggcaata cttccggcgg cattgttcac acggtttgaa ttgatgctgt
    10441 tgaattgcag gccgatttca cccaccaccc acgtcttgtc cattcctccc cagacatcct
    10501 tcgacatcac cagatccaag cccgcgccgg cataccatcc ggcattgcct ccgttcgcgc
    10561 ccgcgatccc ggccgtatcc gtaaaaatct gcccgctccg gtcctggttc aaattcatga
    10621 acccgccttt gaagaacacc atgttgccgg tgccttcctc agccgatgcc ggctgcacac
    10681 aagcaacacc gaacaccgcg gcgatcgcaa gccccgcacc ccacattcca atgttccgca
    10741 cgactctccg aacccgcatc atagtcccct ccttgatgta tcaccgaagc gcctgactgg
    10801 cgcccgggtt ggctccccgc cttgtcctca ccggcctgag gcaggagcag cgcaagcatg
    10861 tggcctactc ttctgaacat cgtggacatt cctcgtctca tccctgcttc gatccaacgc
    10921 atcggcgacg ctcatgccgg tctcacatca gcgaatcgcg cgtctcatca gtcctgcgcc
    10981 cgttgccgct catgcgcgct tcacacttgc agcatgcgcc ggctgattcg ccgtctctgc
    11041 tcggcgctca accgcatctc ggccaaggcg aacagcagcc gctcctcgca ggcgatctgt
    11101 ccacgatagc gacgaatcag cgcgtgaagc tcccccgtca gcgtccgcaa tccgccgaac
    11161 gcttttgacg ccgctgcgtc ttgaccgtcg gtccgctttt tcaccagctg tctcatcact
    11221 gcggccgcgt cggccttcag cgcccggtgt tcgtccaaga aactctggaa ctgctcctgc
    11281 tcctcctgct tccggccgag gattcgccgg agatcgccca tcagcattgc ttcccgtttg
    11341 aaatgcacgt ccacggggcc ggtaaagaat tcgagcagtt ctcgcagcgt ttcccggttc
    11401 gttcccctga ccgcaccgct cccggatgag cgaggactca tcgccgtttc gaccatggcc
    11461 agtcggtcca gaatcattcg gtgctcccgt ttgagcagcg ccaacggatc gacggtgaga
    11521 ctcgttcgcc tgctactttg agacaccatc gttccgggat cccatgtacg gaagctccaa
    11581 gatgcgagca atgcgacagc gtcataccgt gcgcactgta ccgaacaggt aggcgggcac
    11641 acactggcat cggccggtcc gacgcatgat ttatctcagg gttttcgata ggccattgga
    11701 cctatacgca atggcgcagc aaggggcgag atgagcgagt gctaccggga gagcgggatg
    11761 gtggagagcc cga
//
LOCUS       DAORRQ010000093        11714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_262_length_11714_cov_131.601081, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000093 DAORRQ010000000
VERSION     DAORRQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_11714_cov_131.601081"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..832
                     /locus_tag="PK224_18730"
     CDS             227..832
                     /locus_tag="PK224_18730"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRB17840.1"
                     /translation="MWKARSFHADLIEIDLRATSDGHLVLLHDDTIDRTTNKSGHVSE
                     MSLEQIQRLDAGNWQRVPTLEEALDVAGRAIGMILELKVEGIGNEACAIVKRTGFPRP
                     LIYASFLMPELQRVRQTDPDAKIMVLQHRRLPPDPVADVVALDASHVGLHFSTVTPML
                     LQTYHNLGRQVFVYTVNESQDIQRMRNLGVDGIISDFPDRI"
     gene            complement(917..1453)
                     /locus_tag="PK224_18735"
     CDS             complement(917..1453)
                     /locus_tag="PK224_18735"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRB17841.1"
                     /translation="MSFLDVFVRGDFIDPATLVGAAFYAFLFILIAWFLARMIRLGVR
                     RVAYLLSDQAASTLLIRLGQVVVYTLAVILYFNAIPQLHSVGTALLASAGVASILFGL
                     AAQTTLSNLVSGFALLFYQPFEVGDRVQLSAPTGLETGVIDEMTMGYILVKTVDDRHI
                     VVPNSVAAQQIVVKLRAS"
     gene            complement(2597..3310)
                     /locus_tag="PK224_18740"
     CDS             complement(2597..3310)
                     /locus_tag="PK224_18740"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRB17842.1"
                     /translation="MRTVMLGLAGGVLLTAVAVVVSASGFEGLMLLNETSDGTTMQQQ
                     WFLKGDKLRFEETGPDAEKGAMIFDAKKKVMYSLQHGEKIYMEIPTGETSKAAPDRSE
                     DVVVIKTGKRDQAAGYPCEIYHTKDKSDGSTGELCIARGIGNAAMFGMMSAQAGGASL
                     LPGWMRELFKDGGFPIKGVDRDAKGNEEARWEVVNIEKKSLDDALFLPPADYKKQDMA
                     VIRQQSGDAMKEGRSPADR"
     gene            complement(3342..4223)
                     /locus_tag="PK224_18745"
     CDS             complement(3342..4223)
                     /locus_tag="PK224_18745"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRB17843.1"
                     /translation="MQRRWPIVVATVALSLAPLSARAGEFEGVLHMTTSHADTDMKSA
                     MDWYLKGDKGRMEMSRGEGRTSVMIFDATTRTMQMAMPGQKSYMEFSLAGERGEHLAQ
                     AFENQVVERTGKTETIAGYSCEIWRITDKEQHRLKSHVCVAKGFGKAATFWADPKDAR
                     GSSQPGWMKQLAEEGGFAIRSIQYDEAGKESMRMEVTSIDKKSLDAGLFAFPADWAKQ
                     DMSAMQERMKAMREQKGQGKADLSKMLEDMKKRKAARRGAGEPAGEAGPQPDMQEIMR
                     QLSEAMKKQQVQPQGGQ"
     gene            complement(4336..5169)
                     /locus_tag="PK224_18750"
     CDS             complement(4336..5169)
                     /locus_tag="PK224_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRB17844.1"
                     /translation="MEKGDMKAYRIEPGCRLALKRFDPDDTGAYEKNEDGKAKAKAAA
                     DELIATLDQLQERLYANGSRALLIVLQGMDTSGKDGTIKHVMSGVNPQGCKVVTFKTP
                     SAEELSHDFLWRVHQKAPAKGQIGIFNRSHYEDVLITRVHGWVSDKVVTRRFNQIKEF
                     EELLAENGTAILKFFLHISKDEQKARLEARIHDPEKRWKWNSGDLEERKLWGEYMTAF
                     EEVIAATSTEQAPWYIVPANRKWYRNLVVAELVVQALTAMKLSTPPAPKGVNFKTLKI
                     V"
     gene            5389..5874
                     /locus_tag="PK224_18755"
     CDS             5389..5874
                     /locus_tag="PK224_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRB17845.1"
                     /translation="MLKEVTGDILLSTAGAIAHGIAPHDNFKQGLALSLREQWPGMYK
                     DFRHYCQTYNPKPGGAWSWKGPNSPVIINLFTQEPPASDQDHPGKATLPNVNHALQAL
                     KKELQEHQVKSVALPRLATGVGGLEWEKVYPLLQQALKDLNIPVYVYGQYKKGVTAQE
                     A"
     gene            complement(5921..6685)
                     /gene="otsB"
                     /locus_tag="PK224_18760"
     CDS             complement(5921..6685)
                     /gene="otsB"
                     /locus_tag="PK224_18760"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HRB17846.1"
                     /translation="MLSTPGKRVLDRLAGQAEALFAFDFDGTLARIVQDRHAAFLTHP
                     IREALHALAVTAPTAVISGRSLADLSPRVDGIPAHLIGNHGLEGLHTSERVMHQAQDC
                     CRAWLKTVSKDERNLTRAGVVVEDKTYSVTFHYRQACSPQLAREAIFHTVSMLAPAPR
                     LVLGKAVVNAIPSGNLHKGSAMLELMHQLKSSGALYVGDDDTDEDVFSLPDERIVTVR
                     IGKKAASAAQWYLARQSQIGLLLQYLTRVRGRLASA"
     gene            complement(6703..8934)
                     /locus_tag="PK224_18765"
     CDS             complement(6703..8934)
                     /locus_tag="PK224_18765"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HRB17847.1"
                     /translation="MRLSLRFILPLLLVLGVVAYSVVPLVDSLTLKWFTRDLESRSKL
                     LASTMEVPLADLVVSRSRTKIFAYFHKVIQDERLYALGFCDTQQRLLYQTLTYPDQLS
                     CESLAHLAPGSSEVVEFAQGPLHVVVATIQSGGKAQGRLMLVHDMSFVHQRSTDTKWY
                     IFYLFVGLAGLISAVTVLVAQLSWRGWVAGVRAMLTSKGLPPESAGAHSSELHPVAQD
                     LHALVRELEADRRMRDESQITWSPASLKTILHEHLSGDEILIVSNRQPYIHNRRGQKI
                     EVQVPASGLVSALEPVMRACSGVWIAHGNGSADREVVDSRDHVRVPPDHPSYDIRRIW
                     LSPDEESGFYYGFSNEGLWPLCHNAHVRPTFRTSDWEQYVAVNERFAKAVVEEAKTDD
                     PVILVQDYHFALLPKMVREKLPNATIIMFWHIPWPNSESYGICPWRQEILEGLLGSSI
                     VGFHTREHGNHFLSCIDRILEARIDRDSSTVSYGGRLTAVNPYPISIEWPSRWLENQR
                     PVPDCRVHIRERHAMGPDRLVGIGVDRLDYTKGIIERFLAVERLFELQPEWVGRFSFI
                     QIAAPSRTIIDQYQHFHDQVYALAERINKRFGREGYEPIVLKVKHHEPPDVYEYYRAS
                     ELCFVTSLHDGMNLVAKEFIAARDDEQGVLILSQFTGAAQELPEALVVNPYDIDQCAA
                     ALHMALSMPPKEQRARMRSMRGLIQEFNVYRWAGRMLMDAARMRRRIRVMKQVGQASG
                     RGR"
     gene            8973..10769
                     /locus_tag="PK224_18770"
     CDS             8973..10769
                     /locus_tag="PK224_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HRB17848.1"
                     /translation="MVGGMYPYGLIGNCHVSAHIHESGSVDWLCLPRPDSDPVFGRIL
                     DPEGGHFSISSPTSSAQVKTTQRYLPNTNILVTEVSTSKGDTFRITDFCPRFEQYGRV
                     YRPAALFRIVEPLAGTPSIRVSCRPVTGWGKEYARGMRGNSHVRYDIRGEYLRLLTNM
                     PLTYLYEERPFALTEKTYFALTWGLGIEDDLAKVSHEFLDQTTRYWRMWVKHCSIPVL
                     HQEEVIRSALALKLHCYEDTGAILAALTTSLPEEPGGPRNWDYRYCWLRDAYFSLTAF
                     HNLGHFEEMEGFLKFLLNIAYTHEHSRERLAPVYTLSQDLPLPETEHRNWAGFCGSAP
                     VRNHNQAAEHIQNDVYGELVLALTPIFSDNRFYDLRTKDQEQLVANLARLCDRTIAQP
                     DAGLWEIRNGWQEHSFSNLMCWAGLDRAHRMHKAGFLPSLTLDLDAARARAAQALLHA
                     TKDGSLRNGPKDDTYDASLAQAAILGFPNRDVCETTMQSIARALAVQAGGKETGFYFR
                     YLRTDDFGRPQSSFVICSFWVVQGLARLGRMAEAQQIMAQVLTGANHLGLFSEHFVPE
                     TRTQLGNFPQAYSHVGLINAAFAVSPSWTTVL"
     gene            complement(10963..11373)
                     /locus_tag="PK224_18775"
     CDS             complement(10963..11373)
                     /locus_tag="PK224_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17849.1"
                     /translation="MRGTWVAIAGLVLATGCAGLPQTSRTAIIHEVKFEGSLAPADLT
                     VHVGDEIRWVNHRTLPVLIDIPGLNAEMLSCDRGFSGMMGGVSELKELDPSKSASLCF
                     SKPIVISYNARMKSADPGGMKIEPGTIRVVGPAQ"
     gene            complement(11529..>11714)
                     /locus_tag="PK224_18780"
     CDS             complement(11529..>11714)
                     /locus_tag="PK224_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HRB17850.1"
                     /translation="DTYGICSNKFLDREFKTVRYELTVTLHGPDSFSYHEDTQLKMKG
                     RSDLFHHTDTNRVTRVN"
BASE COUNT         2419 a         3744 c         3206 g         2345 t
ORIGIN      
        1 tgtatcaact caccggaggg tgggcccctc ggcgttcttt gcagacttac gcgaaatatg
       61 gtagggaagt ggggttcgaa aagaaagccc ctccctacaa ccggtcaacg caggatcggg
      121 gagggccctc tcccagggca tctcacgcga ggtcgctatc gccaccgtcc ttcgcatcgg
      181 tcatcgggga gccgccggcc acgcccccga gaacaccctc gccgctattt ggaaagcccg
      241 ctcgtttcac gcagatctca tcgaaatcga ccttcgcgca accagcgacg gccatcttgt
      301 gttgctgcac gacgacacca tcgaccgcac caccaacaag agcggacacg tgtccgagat
      361 gtcgcttgag caaatccaac ggctcgatgc ggggaactgg cagcgtgtcc ccacgctgga
      421 ggaagccttg gacgttgcgg gacgcgcaat cggtatgatt ctggaactca aagtggaggg
      481 aatcggcaac gaggcctgcg cgatcgtcaa gcgcaccggc ttccccagac cgttgatcta
      541 tgcgtcgttt ctgatgccgg agctgcagcg cgtgcggcaa accgatcccg atgcgaagat
      601 tatggttttg cagcaccgcc ggctgccgcc ggaccctgta gccgatgtcg tcgcgttgga
      661 tgcctcccat gtcggactcc acttttcgac ggtcacaccc atgctgctgc agacgtatca
      721 caacctcgga cgacaggtct ttgtctacac cgtgaatgaa tcgcaagaca tccaacgcat
      781 gcgcaacctg ggcgtggacg ggatcatctc cgacttcccg gaccgcatct gagctcggca
      841 cagagcagat ggcctacggc tcggaaagag aacggtcccc tcactgtctt ggctataagc
      901 catttgctat tggctcttaa gacgcacgta gcttcaccac gatctgctgg gcagcgacgc
      961 tattcggcac cacgatatga cggtcatcga ccgtcttcac caaaatatag cccatggtca
     1021 tctcatcgat cacgccggtt tcgagtccgg tcggcgcaga cagttgcaca cgatcgccca
     1081 cctcgaacgg ctgatagaac agcaaggcga agccggacac cagattgctg agcgtggtct
     1141 gcgccgccaa gccgaagagg atcgacgcca caccggcact cgccagcaac gccgtcccta
     1201 cggagtgcag ttgtgggatc gcgttgaagt agagaatgac cgccagggta tagaccacca
     1261 cttgccccag cctgatcagc aacgtcgagg ccgcctgatc gctcagaaga tacgccaccc
     1321 ggcgcacgcc caacctgatc atacgagcga ggaaccaggc aatcaggata aataagaacg
     1381 cgtaaaatgc agccccgacc aaggtggcag gatcgatgaa atctccgcga acaaacacgt
     1441 caagaaaaga catagggacc tcccatggca cggcacgtga ttcagaatca gcaactcttg
     1501 ggactgtatg ccatggaaga aggacggaac aagggcggaa gaccgggtcg cataaaatgc
     1561 cacgggcgac cctacactgc ggtgggaggt cgcccgtgga tggcgcttgg gacgccgaaa
     1621 gtcgattata ttgtcggaaa ctcaggaccc gttcgagaag acagggtatc aggatgtccg
     1681 tcgggggata atctatccca ggaaaattgt gtgcctcgtg tttggatcga atccttctct
     1741 ttttgggtgg ctgttatccg gttatcggat agtgccgaga ggcacaagcc agcgaaggat
     1801 cgtcaaaccc gaccgacgat cagcccaccc tcaactctgc gggccgccct agtccggtgg
     1861 cgtaagttct tccatctgtg aacctccccg gttgaggagt ggcgaggtct cccgaacagg
     1921 cgcgcactgt ccatcgcctc gtcacggtta acacagccct tggaatacac aatcccgtct
     1981 tacgctcctt agaaaatcct gtcaatagaa aaatcggccc tagtcatatt tcctgatccg
     2041 aatccgatag gccggttcga gctctcgtta cggcaagggc ccctgcttgg acaggagccc
     2101 ttgggtggcg cttaggacgc cgcgtgctgt ccgtgatgta gcggcccgtg aagacccgcc
     2161 gagtcgaaca agagggaaga acatctcgcc ggagaaaaac agcctgtcat caacaggcga
     2221 cgtgtagaga tcaaaaccag ttctccgctt aggggcatga cgcgatgatg gagatcggtg
     2281 cgtcttcgat gccgcgaggc atagccgaga gagaaccggc cgacagaacc gacgagacgc
     2341 ttgccttcaa tccggcaggc cgtacgggcc aggtggagtc gtatcgttca tcgtccaccc
     2401 tcctttccgt tgcgggactc agtgggagat ctagacggca gccgcgcggt ctgtccggtc
     2461 tcccagctac cacgatggag tcgggccgca aatcctatct acgctcctcg aaaagttctg
     2521 tcaatacagg gtgtcgttgg agtcccgatg gcggcccgac cgaccgccat catccggtgg
     2581 caggcagcat gcagccttac cggtccgccg gtgaccgccc ctctttcatc gcatcgccgg
     2641 actgctgcct gatcacggcc atgtcctgtt tcttgtagtc ggcgggaggg aggaacaatg
     2701 catcgtcgag gcttttcttt tcgatgttca cgacttccca gcgagcctcc tcattcccct
     2761 tggcgtcacg atccacaccc ttgatcggaa acccgccgtc cttaaacagc tcacgcatcc
     2821 accccggcaa caacgaagca ccgcccgctt gcgcgctcat catcccgaac atggccgcat
     2881 tcccgattcc cctcgcgata cagagctcgc cggtgctgcc gtccgacttg tctttcgtgt
     2941 gatagatctc gcacggatac cccgcggcct ggtcgcgttt tccagtcttg atgactacaa
     3001 catcctccga cctgtccggc gcagccttgg acgtctcccc cgtggggatc tccatataaa
     3061 tcttttcgcc gtgctgaagg ctgtacatca ccttcttctt tgcatcgaag atcatggcgc
     3121 ctttttccgc atccggaccc gtttcctcaa accgcagctt gtcgcccttc agaaaccact
     3181 gctgctgcat ggtcgtcccg tcgctcgtct cattcaggag catgaggccc tcaaaaccac
     3241 tcgccgacac gactaccgca acagccgtca gcaagacgcc tcccgccaga cccagcatga
     3301 ccgtacgcat gtcgctctcc tttcagtccg gatgcgcgga gctactgccc gccttgcggt
     3361 tgtacctgct gttttttcat cgcctcgctg agctgcctca tgatctcttg catatccggt
     3421 tgcggcccgg cctcaccggc tggctcaccc gcaccgcggc gcgcggcttt gcgctttttc
     3481 atgtcctcca gcatcttgga gagatccgcc ttaccctgcc ccttttgctc gcgcatcgcc
     3541 ttcatccgtt cctgcatcgc cgacatgtcc tgcttcgccc agtcagccgg gaaggcaaag
     3601 aggccggcat ccaagctctt tttgtcgatg ctggtcactt ccatgcgcat ggattccttg
     3661 cccgcttcgt catactgaat gctccggatg gcgaatcccc cctcctcggc aagttgcttc
     3721 atccagccgg gctgcgaaga cccccgcgca tccttcgggt cagcccagaa cgtcgcggct
     3781 tttccgaacc ccttggccac acagacgtgg ctcttcagtc gatgctgttc cttgtcggtg
     3841 atgcgccaga tctcgcagga atatcccgca atcgtctcgg tcttgcccgt ccgctcgacg
     3901 acctgatttt cgaacgcctg cgcgagatgc tccccacgct cgcccgccag gctgaattcc
     3961 atataagact tctgcccggg catcgccatc tgcatcgttc tggtcgtggc gtcgaagatc
     4021 atgacgctcg tgcgcccttc gccgcgagac atttccatgc ggcccttgtc acccttgaga
     4081 taccagtcca tcgcgctctt catatcggtg tccgcgtgac tggtcgtcat gtggagcacg
     4141 ccttcaaact caccggcgcg ggcagagagt ggagcgagac tcaaggcaac ggtcgcgaca
     4201 acaatcggcc atcttctctg catgatccac actcctggtt agacgagcgc ctgcaagaca
     4261 aactgcagtg tagccgtgtg tagtggccgg caccagcctg gaccggccag gcttgcccct
     4321 gcgagcaacc acgccctaaa caatcttcag cgtcttgaaa ttcactccct tcggagccgg
     4381 aggggtcgag agcttcatcg ccgtcaacgc ctgaacaacc agttccgcca ccacgagatt
     4441 gcgataccac tttcgattgg ccggcacgat gtaccagggg gcctgttcgg tgctggtcgc
     4501 cgcgatcacc tcttcgaacg ccgtcatata ctcgccccac agtttgcgct cttccagatc
     4561 cccggaattc catttccacc gtttttcggg atcgtgaatg cgggcttcca ggcgggcctt
     4621 ctgttcgtct ttcgaaatat ggaggaaaaa tttcagaatg gccgtcccgt tctcggcaag
     4681 cagttcctcg aattccttga tctgattaaa gcggcgggtc acgactttgt ccgagaccca
     4741 gccatgcacc cgcgtaatca acacgtcttc atagtgtgaa cggttgaaga ttccgatctg
     4801 gcccttggcg ggcgccttct gatgcacgcg ccagaggaag tcgtgggaga gctcttctgc
     4861 ggaaggcgtt ttaaaagtca ccaccttgca gccctgcgga ttaaccccgg acatgacgtg
     4921 cttgatcgtg ccgtccttgc cgctggtgtc catgccctgt agcacgatca acagggcgcg
     4981 cgagccgttg gcatagagac gctcctgcaa ttgatcgagt gtcgcaatga gttcgtccgc
     5041 cgccgccttg gccttcgcct ttccatcttc gttcttctcg tacgccccgg tgtcatcggg
     5101 atcgaaccgt tttaatgcca gtcggcagcc gggctcgatg cgatacgcct tcatgtcacc
     5161 cttctccaca atagtgcctc cttccactga ccaagaccag cgcttcctcg cacgatctct
     5221 tcatgaaaca gaggcctgca cgccgacatg gcgagcgact gcctcggcga cagcacgagc
     5281 acactcccgg tcgcgaatta aatttcttcc cccgctccat tgattgaaac ggacgcagcc
     5341 ttatgtgata agacaggaac ttattcccac atcaaggagg cgtcagtcat gctcaaggaa
     5401 gtgaccggag acattctctt atccaccgcc ggcgcgatcg cacacggcat tgccccccac
     5461 gataatttca agcagggctt ggccttgtcg cttcgtgagc agtggccggg catgtacaaa
     5521 gattttcgcc actattgcca aacctacaat cccaaaccgg gcggcgcctg gtcctggaaa
     5581 ggcccgaatt ccccggtcat catcaatctg tttacgcaag agccgccggc cagcgatcag
     5641 gatcatcccg gaaaggccac cttgcccaac gtcaatcatg cgctccaggc gttgaagaaa
     5701 gaactgcagg agcatcaggt gaagagcgtg gcgctgcctc gtctggcaac gggagtcggc
     5761 ggactggaat gggagaaagt ctatcccttg ctccagcagg ccttgaaaga cctgaatatt
     5821 cctgtgtacg tctacggtca gtacaaaaaa ggggtgacgg cccaggaagc ttagcaggca
     5881 caaccaccgg tactgaggcc agggcaatga atatcaggag ctaggccgac gccagacggc
     5941 ctcgtacccg ggtcagatac tgcagcaaga gtccgatctg cgactgccgc gcaagatacc
     6001 attgtgcggc agacgcagcc ttctttccga tgcgcaccgt gacgatgcgc tcatccggca
     6061 acgaaaacac atcctcatcg gtatcgtcat cgccgacata cagagcaccg gaactcttca
     6121 gctggtgcat caattccaac atcgccgacc ccttgtggag attgccggag gggatggcat
     6181 tcaccacggc tttgcccaac acgagcctgg gcgcaggcgc caacatcgac acggtatgaa
     6241 agatcgcctc acgcgcgagc tgcggtgaac aagcctgccg ataatgaaac gtgaccgaat
     6301 aggtcttgtc ctccaccacc actcccgctc gcgtcagatt tcgctcgtct ttggacaccg
     6361 tcttcaacca ggcgcggcaa caatcctggg cctggtgcat gacccgctcc gacgtatgca
     6421 gcccttcgag cccgtgattg ccgatgagat gcgcggggat gccgtccacg cgcgggctga
     6481 ggtcggcaag tgagcgtccc gaaatgaccg ccgtcggcgc cgtcactgcc agggcgtgca
     6541 gggcctcgcg aatagggtgg gtgaggaaag cggcatgccg gtcttgaacg attcgcgcca
     6601 gcgtgccgtc gaaatcgaac gcaaacaacg cctcggcctg gccggccagc cgatcgagga
     6661 cacgcttgcc tggagtgctg agcacgtcct gcatcggtgc gattaccgcc cccgtcccga
     6721 agcttgtccc acctgcttca tcacgcggat acgacggcgc atccgggccg cgtccatgag
     6781 catacgaccg gcccaccggt agacattgaa ctcctgaatc agtccgcgca tgctccgcat
     6841 acgggcgcgc tgttccttcg ggggcataga gagcgccatg tgcaacgccg ccgcgcactg
     6901 gtcgatatcg tagggattca ccaccaacgc ctccggcagc tcctgcgcgg ccccggtgaa
     6961 ctggctgaga atcagcacgc cttgttcgtc gtcgcgcgcc gcgatgaatt ccttggccac
     7021 caggttcatg ccgtcatgaa ggctcgtgac gaagcagagc tcggaggccc ggtagtactc
     7081 atacacatcc ggcggctcgt ggtgtttcac cttcaacacg atcggctcat acccttcgcg
     7141 gccgaatcgc ttattgatgc gctcagccag cgcgtagact tgatcgtgga aatgctggta
     7201 ctggtcgatg atcgtccggc tcggcgccgc gatctgaatg aaggaaaacc tgccgaccca
     7261 ctcgggctgc agctcgaaca atcgttcgac ggcgagaaat cgctcgatga tgcccttggt
     7321 atagtccaag cggtcgacac cgatcccgac cagccgatcg ggtcccatcg catgacgctc
     7381 gcggatatgc acccggcagt ccggcaccgg ccgttgattc tccaaccagc gcgacggcca
     7441 ctcaatggag atcgggtagg gattgaccgc cgtgaggcgc ccgccgtagg acaccgtgga
     7501 gctgtcgcgg tcgatgcgcg cttcaaggat gcgatcgatg caggacagga aatggtttcc
     7561 atgctcacgg gtgtggaacc cgacgatgct gctgccgagc aagccttcca gaatctcctg
     7621 ccgccacggg cagatgccgt agctctcgga gttgggccag ggaatatgcc agaacatgat
     7681 gatggtggcg ttcggcaact tctcacgcac catcttcggg agcagcgcaa agtggtaatc
     7741 ctgtacgagg atcaccggat catccgtctt ggcctcttcc accaccgcct tcgcgaaccg
     7801 ctcgttgacg gccacatact gttcccagtc ggaagtcctg aacgtcggac ggacatgcgc
     7861 attgtggcat aagggccaga ggccctcgtt ggaaaaccca taataaaacc cggattcttc
     7921 gtcgggcgac aaccagatgc ggcgaatgtc gtaggaagga tggtccggcg gcacccgtac
     7981 gtggtcccgg ctatccacga cttcccggtc cgctgaaccg ttgccgtgag cgatccacac
     8041 gcccgaacaa gcccgcatca ccggctcgag tgcggacacc agcccgctgg caggcacttg
     8101 cacttcgatt ttctgcccgc gccgattgtg gatgtagggc tgtcggttgg agacaatgag
     8161 aatttcgtcg ccagagagat gctcatgcag aatggttttg aggctggcgg gactccaggt
     8221 gatctggctt tcatcgcgca tccggcgatc ggcttccaat tcccgcacca gcgcatggag
     8281 atcctgcgcc accggatgca gttcggacga atgggctccg gcggattcag gcggcaaccc
     8341 tttgctggtc aacatggccc gcaccccggc cacccagcct cgccagctga gttgcgcgac
     8401 gagcacggtc accgcggaaa tcaacccggc gagtccgacg aagagataaa agatgtacca
     8461 cttcgtatcg gtgctgcgct ggtgcacaaa actcatgtcg tggaccagca tcagccggcc
     8521 ctgcgctttg cctccggact gaatggtcgc gaccaccacg tgcaacggtc cttgcgcaaa
     8581 ctccaccact tccgaagagc ccggcgccag atgcgccaga ctttcgcagg acagctggtc
     8641 gggataggtc aacgtctggt agagcagacg ctgctgggta tcgcagaaac ccagcgcata
     8701 gagccgctcg tcctgaatga ctttgtgaaa gtaggcgaag atcttcgtgc gtgaacgcga
     8761 cacgaccagg tccgccaacg gcacttccat cgtgctggcc agtagtttgg atcggctttc
     8821 gaggtcgcgc gtgaaccact tcagcgtgag cgaatcgacg aggggcacga cgctataagc
     8881 cacgactccc aaaaccagca agaggggaag gataaaccgg agggacagcc gcatttttgc
     8941 tcctacagtt tactttgaga cgaaaatctc ttatggtcgg gggcatgtac ccctacggac
     9001 tcatcggcaa ttgccacgtc tccgcccata ttcatgaaag cggatctgtg gactggctct
     9061 gtctgccaag accggatagc gacccggtct tcggtcgaat cctcgatccg gagggagggc
     9121 acttttccat atcaagccct accagctccg ctcaagtcaa gacaacgcaa cgctaccttc
     9181 ctaatacaaa tatcctggtg accgaggtct ccacgtcaaa gggcgacacc ttcaggatca
     9241 cggacttctg cccgcgcttc gaacagtacg gccgcgtgta ccgtcccgcc gccctctttc
     9301 gcatcgtcga accgctcgcc ggcacgccct ccatccgcgt gagttgccgc ccggtcacgg
     9361 gatggggaaa agaatatgcc cgcggcatgc gcggcaacag ccacgtccgg tacgacatcc
     9421 gcggggagta cctccggttg ttgaccaaca tgcccctgac ctacttgtac gaggaacgcc
     9481 ccttcgccct caccgagaaa acgtacttcg cactcacctg gggcttgggc atcgaagacg
     9541 atctcgccaa ggtcagtcac gagttcctcg accagacgac ccgctactgg cgcatgtggg
     9601 tgaaacattg ctcgatcccg gtgctgcacc aggaagaagt cattcgttcc gcgctcgcac
     9661 tgaaactcca ttgttatgaa gacacgggtg ccatcctggc cgccttgacg accagcctcc
     9721 cggaagaacc gggcggcccg cggaattggg actatcggta ctgctggctg cgcgacgcct
     9781 atttttctct gaccgccttc cacaatctcg ggcacttcga agagatggaa gggtttctca
     9841 agttcctgct caacatcgcc tacacgcatg aacattcgcg ggaacgactc gcgcccgtgt
     9901 atacgctcag ccaggatctc ccgctgccgg aaaccgaaca ccggaactgg gccggcttct
     9961 gcggaagcgc gccggtgcgc aatcacaatc aggccgccga acatatccaa aacgatgtct
    10021 acggcgaact ggtcctcgca ctgaccccga tcttctccga caatcgtttc tacgatctgc
    10081 ggaccaaaga tcaggaacaa ctcgtcgcca acctcgcccg actctgcgac cgaaccatcg
    10141 cccaaccgga tgcggggttg tgggagattc gcaacggctg gcaggaacat tcgttctcga
    10201 atctgatgtg ttgggccgga ctcgaccggg ctcaccgcat gcacaaggcg ggatttctcc
    10261 cctcgttgac gctcgacctc gacgcggcgc gtgcgcgtgc ggctcaggcg ctgttacacg
    10321 ccaccaaaga cggctcgctg cgcaacggtc ccaaggacga cacctacgac gcttcgctgg
    10381 cacaagcggc gatcctcggc tttcccaacc gagacgtgtg cgaaaccacg atgcagagca
    10441 tcgcgcgcgc gctggccgtg caagccggcg ggaaggaaac cggcttctat ttccgctacc
    10501 tgcgcaccga cgatttcggg cggccgcaat ccagctttgt catctgttcc ttctgggtcg
    10561 tccagggact cgcgcgcctg gggcgaatgg cggaggcgca gcagattatg gcccaggtcc
    10621 tgaccggcgc aaaccatctg gggctcttct ccgaacattt cgtccccgaa acccgcactc
    10681 agcttggaaa ctttccgcaa gcctattccc acgtcggtct gatcaacgcc gccttcgccg
    10741 tcagtccctc ctggacgacg gtgctgtaac cctcacgcct gtgcagtcag acggccgcct
    10801 ctgccccgta caacagtgcc gcgccgacag tgcgcgccta ccgagacgac atccggcaca
    10861 atcgaaatct ctggtctgat gatctaactc gaaccccgga gccctgtgac aagccccggg
    10921 gcgaaggaga gggtaacgcg gcgtaagccg ggtaagcaca gactactggg cggggccgac
    10981 gacccggatg gtgccgggct cgatcttcat gccccccgga tcggctgatt tcatgcgggc
    11041 attgtagctg atgacaatag gcttgctgaa acacaagctc gcacttttgc tcgggtctaa
    11101 ttccttcaac tcgctcaccc ctcccatcat gcccgagaaa ccgcgatcgc aagacaacat
    11161 ctctgcattc aggccgggaa tatcgatgag cacggggagc gtccgatggt tcacccagcg
    11221 tatttcgtca ccgacgtgca cggtcagatc tgccggcgcc agactgccct caaatttgac
    11281 ctcatggatg atcgccgttc tcgaggtctg cggcagcccc gcacaaccag tcgccaaaac
    11341 caatcctgct atcgccaccc acgtgcctcg catatcgcgc ctccttcctg aaaacaattg
    11401 tacttaagac tctcagaatc tgagcggtct gttcttactc tttcgaatct acccagttac
    11461 tctaccacgt tccacgagct cacttacaag ctggggactc agtattttcg cgtggaaacc
    11521 tacctgcatt agttgactcg tgtcacccga ttggtatcgg tgtggtgaaa caagtcgctc
    11581 cgtcccttca tcttcagttg cgtatcttcg tggtagctga aactgtcggg gccgtgcagg
    11641 gtcaccgtga gttcataccg gaccgtcttg aactcgcgat ctagaaactt attcgaacag
    11701 atgccatacg tatc
//
LOCUS       DAORRQ010000094        10929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_275_length_10929_cov_97.006989, whole genome shotgun sequence.
ACCESSION   DAORRQ010000094 DAORRQ010000000
VERSION     DAORRQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_275_length_10929_cov_97.006989"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..363)
                     /locus_tag="PK224_18785"
     CDS             complement(52..363)
                     /locus_tag="PK224_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17851.1"
                     /translation="MSSLLAECPPRNRRRPNALTYDEPAEGFRYDPASQVQQILHKLG
                     ASGPTINQAAYTYNGVGNRDSLTDRRGVQNFGYDNLDRLTSASHPLLGTQQSFAYDPK
                     T"
     gene            complement(482..949)
                     /locus_tag="PK224_18790"
     CDS             complement(482..949)
                     /locus_tag="PK224_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17852.1"
                     /translation="MIRKAYTVALSAMLFACMACEKAEIADRYYLNYEQLRASAEPGN
                     WVPTFIPRSAVEIRARYKIDTGAELVTFYVAKPDDLSVMSHCKNSTKSSTDLPPKGFL
                     NVTWWPESLFQNQDRNESVARYEFYTCQGQTFLALEREGGGTRAFYWSISVAQ"
     gene            complement(946..2862)
                     /locus_tag="PK224_18795"
     CDS             complement(946..2862)
                     /locus_tag="PK224_18795"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRB17853.1"
                     /translation="MDDYVSIPDSAVLDSLSTNATLDAWIKPELPLGAEAWLFARRDP
                     FVSEGFSVALLQDGRMQLTVRTTTSPTVSGSTFRSTASVITFDQWQHVAAAVDTVAGT
                     AQIWVNGQAISLTAVFGPATLSGTLFNVNQQYLGRRQDPNTAGGGGHFKGQLDEVRLY
                     GRTLTLAEVQSRVQNQPVATYVYDALSRRTSLTLPNGIQTTYTYDPASQVTAISHQLT
                     ALSSQINKAEYGYNAVGNRTSLTDRRGPQNFGYDNLDRLTSASHPLLGTPQAFAYDPV
                     GNRATGGSVVNAGNQLTADATHSYQYDDNGNLTRKTLLATGNYTQYTYDAENRLIKVE
                     DFAAGNPTPAFTSTYRYDGLGRRIEKVANGQTKRYIYDGEDILLEYDGTSTLQARYTH
                     GPGIDEPIAVTKGSSTFFYHQDGLGSVTDLTDSTGATAKSYAYDAYGSILESPGTLEQ
                     PYTYTGREFDAESGLSYYRARNYDPSSGRFLQKDPIGFSGGDINLYNYVGNNPARWID
                     PLGLCYPGDFVGGLGDMISGRSDMMDAGTKGADAYFHCRANCQASRRGLGGYHAAVIA
                     SALREMIQNEPPADRARDEAANAQGQCAGNNNPSADCYKGCDNLIPPSGIPQRHLPPH
                     ADPRHRYQPAGTPP"
     gene            complement(3127..3444)
                     /locus_tag="PK224_18800"
     CDS             complement(3127..3444)
                     /locus_tag="PK224_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17854.1"
                     /translation="MKITKSQQSILSRPRRYVALIILLFSAPTCGGLGVLTADVFAKL
                     IQGGPEVVFVRWAAPANVLEAISGYVGGLLGFAMGYWLWKRVMVRTGVLSPAQLRELY
                     KSE"
     gene            complement(3621..10850)
                     /locus_tag="PK224_18805"
     CDS             complement(3621..10850)
                     /locus_tag="PK224_18805"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRB17855.1"
                     /translation="MAFSAETEVRAEEASTIEMLQQPEMPIGNVGGEAHADPFTGAAT
                     MSIPIEVPPGRNGIQPNLSLVYMSGNGNGWVGMGWKLEIGAVERQVRYGLNYSANDFV
                     ARMSGANSELVQAPAPAQSNEYRAKIERGFVRFRKLSAGDGQAYFEATDKKGVRYFFG
                     QTAASRMADSANPSRISKWLLDRIEDRDRNYMVATYLSDQGQVYLSRIEYAGNGTTAP
                     TNVVKFYLEDRSDAPSMFTTNYVVKTVKRLKTIEVQASGNSVRAYKMTYGQTSSIPSS
                     VLTSVTQFGSDATINGTGAITNEATATRLPSTSMEWSAGPATFGPEGGYTGLWLGAGA
                     CSNVNALLVNTRYGDFNGDGKTDILCEDRYTGTYYVALSSGELFNDGGGWTGTWLGNG
                     NAASGACGLPPFQYRDPAHPAAPPYNDGIRTQYADFNGDGKTDLLCEDRYTGTFYVAL
                     SDGTKFGNGAGYTGIWLGNGSYAGGACAFPNPANSIWAARLNTRYADFNGDGKTDLLC
                     EDRNTGTYYMALSDGTKFGSGGGWTGTWLGNGNAANGACGPALNVTTRYGDFNGDGKM
                     DLLCEDRNTGTYYVGLSDGTKFGNGAGWTGTWLGNGNIASGACIAGANITTQYGDFNG
                     DGKIDLLCEERNSGTYYVALSDGTKFGSGGGYTQEWMGNGNASNGACVAATNVTPQYG
                     DFNGDGKADLLCENRNTGTYYVALSDGTRFGNGAGYTGTWLGNGDPASGACGLATGLF
                     TRYGDFNGDGKTDFLCEDRTNGNYWVAKSGNHSTMVAGQLLSVSNGVGGRRAITYKPS
                     TRYTNTQLPYSIQTVSSIVSCDNWNGTACAGISGTANYDYSGGHHHFAEREFRGFNHV
                     KVTAPAGPNGEQAITETWFHQGNDLGVDSNNPNVPNGYTKGMPYRTKVTDATGKLYSE
                     TTTAYVADNDLAAPWYTPQSQIDTYLCESGTCATHTRTTFEYDHQYGNLSLETHYGDL
                     ANPNDDRAIYRTYSDNVADWIVGLPTRESHYNSISTVSVNLVAHKTFFYDGTGGCASP
                     NGSTTPTKGHLTKATNWLSVTGAEYDVWGNITCTRDANGNVSTVTYDSTKTFPKMTTN
                     PLGHVTTTQYYGVDGQVVDKGLYGQIKSVTDPNSQVTVMEYDALGRKTKVTAPDGGIT
                     SVAYNYGTGFGVGTQHVLSTTSGAGIVTPLSSWSFFDGLGRSIRKEATGPDGKVIVTE
                     VQYDSRGAVRQKSLPYFKTIETVAGRWSTSSHDALGRVIRLDSPDGTRSVVCFNDWVT
                     VTIDAADHRTRVTKDAYGRTVRVDEYQGTFSTCTTEVGSHYATTVYQYDSRDNLIAVT
                     DAKGNVTTIAYDEQSRKKNMRDPDMGYWIYGYDANGNLTDQTDAKGQVTWFRYDALNR
                     QVQKDFVAQKALGSGDVRYTYDGTTNNRKGRLQQIWDASGTVVLQYDSMGRVLRSDRT
                     LDGTTYTTQSTYDGLGRLLTVTYPGTPSKTISYAYNGPVLDKVFEGTTTYIQYTNYNA
                     LGQAGTTSYGNGVSTTMTYANANNTVCSQQNFRLCTLKTTGPGSGGGGGGGTSTTYNA
                     VADFSGTQGFHGWYYLSSSGAQLTWNGNFWSGSDGYISLWDDGGHPGDSTDLVRRWVA
                     PQTGSVQITGNTFDGDTACGVDGVQVTIKKNGAVLWQQTIAAGDTTGYSFNLSNTVAV
                     GDQLDFVTNKLTTSSCDSTVFTATIVLTTAGGGGGGSSTSYNAVTDFSGVQGQQGWYY
                     LSSVGTQLTWNGNFWSGSDGYIGLWDDGGHPGNSTDVVRRWVAPQAGSIQITGNTFDG
                     DTSCGVDGVLVTIRKNGTVLWQQTIAAGNTTGFTFNLSNTVAVGDQIDFVTNKLTTSA
                     CDNTVFTPTILLSAGGSSGTVYQDLRYVYATNGNVNDIYDNLVAVGAGDQHLGYDGLD
                     RLTLANGPYGTNGANASLTYAYDELGNLTFNSQVGTYTYPLSGSSSVRPHAVTTAGSN
                     TYSYDANGNLTSGAGRTLTYNLENKPLTITIDGQTTTFVYDGDNGRVKKIAGTTTTRY
                     ISKLYECDNTSCSRMIFADGRRIATIGASGAVYYYHTDHLGSSSVITDSAGTKVQLLT
                     YFPFGAVRTNQSTVTPTIDVPYKYTGQEFDASTGLYDYGARQYDPILARFVSADPIQI
                     PIGRVDLLNRYTYANDNPIRLTDPTGQTPTIPDLLIGAGIGMTAGYTANLTKSGVDRA
                     IGFVVGGIVGGGVGLVSPSLSRVAAGEVSALAVSGWNAAGSYLQQVGQNVVTAWYGGQ
                     TLDGYWALTSVNWYQVGGAFVVSGPLSVIGGGYLNPIITGASGSRILGGSAEVIYSGL
                     NQGVGDLGLNVAGGILNRSGSPMPLPPGNNPIFDIQPDKGLSAFVVTGHSQPYGVHGY
                     QYDLPGSSSYSGQSSGSVTQAGDLSGNGSGGAAMGPDFSFDLFEF"
BASE COUNT         2295 a         3169 c         2816 g         2649 t
ORIGIN      
        1 tggcgggtcg cggtcagtca gtcggcttga ttttcaagac agttgctgac cctatgtttt
       61 gggatcatag gcgaagctct gctgtgtccc taatagcgga tggctcgcac tcgtcaatcg
      121 atccaagtta tcgtagccaa agttctggac gccccgccga tccgtcaaac tgtcccgatt
      181 gccgacgcca ttataggtat aggcggcctg gttgatcgtc gggcccgagg cgccgagctt
      241 gtggagaatc tgctggacct gagaagccgg atcatacctg aagccctctg cgggttcgtc
      301 gtaggttaag gcgtttggcc ttctgcgatt tcgtggcgga cactcggcga gcagcgagga
      361 cataggagga gattctagca gctcgagccc aattcacaag agagaagatg caggaagagg
      421 gaagaggaga tgatgctgaa gatgatgtgg acacagggct gaagaaacct ttagtttttc
      481 tctattgggc tacactgatg ctccaataaa atgcccgcgt gcccccaccc tctcgttcta
      541 gggcaagaaa tgtttgtcct tgacaggtat agaactcgta gcgcgcaacg ctttcattcc
      601 gatcctgatt ttgaaacaat gattctggcc accatgtaac attgagaaat cccttcggag
      661 gcaagtcggt agagctctta gtagaatttt tgcaatgact catcacggat aggtcgtcag
      721 gcttggcaac atagaacgtt accaactccg cgcccgtatc aatcttgtat ctagctcgga
      781 tttctacagc cgaacgtgga atgaacgtcg gcacccaatt ccctggttcg gccgaggcac
      841 gaagttgttc atagttgagg taataccgat ctgcaatttc tgctttttca catgccatgc
      901 aggcaaacag catcgcactt agagcaacag tgtaagcttt tctaatcatg ggggcgtacc
      961 tgctggctgg taacggtgtc gcggatcagc atgtggaggc aggtgccgtt gcggaattcc
     1021 agacggcgga attaaattat cacatccctt gtaacaatcg gcacttgggt tgttatttcc
     1081 tgcacattga ccttgagcat tcgcggcttc atctcgggca cgatctgctg ggggctcatt
     1141 ctggatcatt tcccgaaggg cactggctat cacagcagca tgatagccac caaggcctcg
     1201 ccttgaagct tggcaattcg ctcggcaatg gaaatatgca tcggcgcctt tggtgccagc
     1261 atccatcatg tcgctacgcc cgctgatcat gtcgccaagg ccgccaacga aatcgcctgg
     1321 atagcacaat cctagcggat caatccacct cgctggatta ttgcccacat agttgtagag
     1381 attaatgtct cctccactga atccgattgg atccttctgc aagaatctcc cgctcgacgg
     1441 atcgtagttg cgcgctctat agtaggagag cccagactcc gcatcgaact ctctccccgt
     1501 gtacgtgtac ggctgctcca gcgtgccggg gctctccaag atgctcccgt atgcatcgta
     1561 ggcgtaactc ttcgccgtcg cgcccgttga atccgtcaag tccgtgactg acccgaggcc
     1621 gtcttggtgg tagaagaacg ttgagcttcc tttggtgacc gcgatgggtt cgtcgatccc
     1681 tgggccgtgg gtgtatctgg cctggagcgt acttgtcccg tcatattcga ggaggatgtc
     1741 ttcgccatca tagatgtacc gtttcgtttg cccattcgcc accttctcaa tccgccgacc
     1801 taatccatca tagcgatatg tactcgtgaa ggctggggtc ggattccccg ccgcgaagtc
     1861 ttcgaccttg atcaggcgat tctccgcatc gtaggtgtac tgggtatagt tccccgtggc
     1921 gagcagcgtc ttgcgcgtca gattcccgtt atcatcgtat tgatagctgt gcgtcgcatc
     1981 ggcggtcagc tggtttccgg cgttcaccac gcttccgccc gttgctctgt tgcccaccgg
     2041 atcgtaggca aaggcttggg gtgtgcctaa caacggatgt gaggctgacg tcagccgatc
     2101 caagttatcg tagccgaaat tctgcggacc tcgtctgtcc gtcaaactcg tccggttccc
     2161 cacggcgttg tacccgtatt cggccttgtt aatctgactg ctgagggctg tcagctgatg
     2221 gctgatcgcc gtcacctgac tagcgggatc ataggtatag gtcgtctgga tcccgttcgg
     2281 gagcgtgagg ctggtccggc gcgagagcgc gtcatagaca taggtcgcca cgggctgatt
     2341 ctgtacccgg ctttgcacct ccgccagggt gagggtgcgc ccatagagtc gcacttcatc
     2401 cagctggccc ttgaaatgcc cgcctccacc cgccgtgttc ggatcttgcc gtcggccgag
     2461 atactgctga ttcacattga agagcgtgcc gctcaaggtc gctggcccaa agacggcggt
     2521 caggctaatg gcctggccat tcacccagat ctgggccgtg cctgccacgg tatccacggc
     2581 cgccgccaca tgttgccatt ggtcgaaggt aatcacgctc gcagtcgagc ggaacgtgga
     2641 gccggacacg gtggggctgg tggtcgtccg gacggtcagc tgcatccggc cgtcttggag
     2701 gagtgccacg gagaaccctt cgctcacgaa gggatcacgc cgggcaaaga gccaggcttc
     2761 ggccccaagg ggcagctccg gcttgatcca ggcgtccagg gtggcgttgg tactgaggct
     2821 atcgagcacc gccgaatccg ggatactcac atagtcatcc acgccggcga tccgccgctg
     2881 gcgccttttt gagccatcct cgacacagat cctcattcac cttcggtaag gtaccgtctc
     2941 cattttgttg tggaggaggg cccgacgcca gaggtggctc ctatgtttta acagcatgtt
     3001 ccagttctaa tgtggtgcct tgcgttttct gcctatgcgg gggtacgcca tgcaggctgg
     3061 ttgtcgcagc acatcaggtc tggcgccttg ccgtcaagca tgtcttcgac gatctcttct
     3121 tacaggtcac tcggatttgt ataactccct aagctgcgca ggagagagga ctccggttcg
     3181 aaccatgacc cttttccaaa gccaatagcc catggcgaat ccaagcaacc cacccacgta
     3241 gcctgagatt gcctcgagaa cgttggctgg agctgcccat cttacgaaaa caacctccgg
     3301 accgccctga attagctttg caaaaacatc tgcagttagt actcccaatc caccgcatgt
     3361 tggggcagag aatagaagaa ttatgagagc tacatatctc cgtggtcgac tcagtatgct
     3421 ctgttgagat ttagtgatct tcatatttaa ctcaaatccg aagaacgttt tttcttacac
     3481 aaggcttctc gtcagagcgt aatgcattaa cccaagtcct ctgctcagtc ggccaggatc
     3541 acgtcgaatc agcaaaccaa acggaatggt aatgactata tattacctac caaggtcaga
     3601 tctcatttta tgctcttgac tcagaattca aagagatcaa aagaaaaatc aggtcccatg
     3661 gccgctccac cactgccgtt tcctgagaga tcaccagcct gcgtaacgct accgcttgat
     3721 tggccgctgt agcttgagga cccaggaaga tcatattggt atccgtgaac cccataaggt
     3781 tgagaatgcc cagtgactac gaatgccgaa agccccttat ccggctgaat atcgaagatc
     3841 gggttgtttc cgggaggtag gggcatgggg ctaccactcc tatttagaat ccctcctgcc
     3901 acgtttaggc caagatctcc tactccttgg tttagaccag aatagataac ttctgcagaa
     3961 ccccctagaa ttcgactccc agatgctcct gtgattattg gattgagata tccaccgcca
     4021 ataacactca aagggccgct gactacgaag gcgccaccca cctgatacca attgacactt
     4081 gttaacgccc aataaccatc aagagtttgg ccgccatacc aagcagtcac cacgttctgt
     4141 ccaacttgct gcaagtagct tccggctgca ttccatccac tcacagctaa ggcactgacc
     4201 tcaccagccg caacccttga aagtgatggc gaaactaggc ctacgcctcc accgacgata
     4261 cctcctacaa cgaatccaat cgcccgatct acgcccgact tggtgaggtt agcggtataa
     4321 ccagcagtca ttccaatacc tgctccaatc aaaagatccg gaatggttgg agtttgccct
     4381 gttggatcag tgagtcgtat gggattgtcg ttagcatatg tgtaacgatt caagaggtcc
     4441 acgcgcccga tcggaatttg tattgggtcg gccgaaacaa accgtgccaa aatcggatca
     4501 tactgcctgg ctccgtaatc atagagccca gtacttgcat caaactcctg ccctgtatat
     4561 ttgtacggca cgtcaatggt tggcgtgacc gtcgattggt tcgtgcgcac ggcaccgaac
     4621 ggaaaatagg ttagcaattg gactttggtc ccggcgctat ctgtaatgac gctcgacgaa
     4681 ccgaggtgat cagtatggta atagtaaacc gccccactcg ccccgatggt cgcaatgcgt
     4741 cgaccgtcgg caaagatcat ccgggaacaa ctcgtgttgt cacattcata gagtttgcta
     4801 atgtaccggg tggtcgtggt ccccgcaatc ttcttgaccc gcccgttatc gccatcatag
     4861 acaaacgtcg tcgtctgccc atcgatcgta atcgtgagcg gtttgttttc caggttgtag
     4921 gtcaacgtcc gacccgcgcc gctggtcagg ttgccattcg catcgtagct gtaggtattg
     4981 ctgccagccg tcgtgaccgc atgcgggcga acactcgagg acccgcttag cggataggta
     5041 taggtgccca cctgggaatt gaaggtgagg ttacccaatt catcatacgc ataggtgagc
     5101 gacgcgttcg ccccattcgt cccataaggg ccattggcga gcgtcaagcg atcgaggccg
     5161 tcataaccca ggtgctgatc gccagcgcct acagccacaa ggttgtcata gatatcgttc
     5221 acattgccgt tggtcgcata cacgtagcgg aggtcttgat agacagtgcc gctgctcccg
     5281 ccggcactga gcagaatggt tggagtaaac accgtattgt cacaagcgga ggtcgtgagc
     5341 ttgttcgtca cgaaatcaat ctgatcgccc accgcaacgg tattcgagag gttgaaggta
     5401 aaccccgtcg tatttcccgc tgcaatggtc tgttgccaga ggaccgtccc attcttccga
     5461 atcgtgacta agactccgtc gactccacag gaggtgtcgc catcgaacgt gtttcccgta
     5521 atctggatgc ttcccgcttg tggggccacc catcgacgca cgacatcagt gctattgccg
     5581 ggatgcccgc catcatccca gaggccgatg tacccatctg acccgctcca gaaattcccg
     5641 ttccaggtca actgcgtgcc gaccgagctc agataatacc agccctgctg cccctgcacc
     5701 ccggagaagt ccgtcacggc gttgtaggac gtactactgc cgccacctcc cccaccagcg
     5761 gtcgtgagca caatggtcgc ggtgaacacc gtgctgtcgc aggaggaggt cgtgagttta
     5821 ttcgtgacga agtcgagttg atcaccgacc gcaaccgtgt tggagaggtt aaagctatag
     5881 ccagtggtgt cacccgccgc gatggtctgt tgccagagga cggccccgtt ctttttgatg
     5941 gtcacctgca ccccatccac cccgcaggcg gtgtcgccat cgaaggtgtt gcccgtaatc
     6001 tgcacactgc ctgtttgcgg ggccacccaa cgtcgcacga gatcggtact gtcaccggga
     6061 tggcccccat catcccaaag actgatgtac ccgtctgacc cgctccagaa attgccgttc
     6121 caggtcagct gagccccgct ggagctcaga tagtaccacc cgtgaaaacc ttgggtgcct
     6181 gaaaaatcgg ctacggcatt gtaggtagta ctggttccac cacccccgcc tcctccgcta
     6241 cccggtccgg tggtcttgag ggtacacagc cggaaatttt gctggctgca gacggtattg
     6301 ttggcattgg cataggtcat cgtggtcgag acgccgttac cgtaggaggt cgtgcccgcc
     6361 tgcccgagcg cattgtagtt ggtgtactga atataggtgg tggtgccttc aaacaccttg
     6421 tccaggaccg gtccgttata ggcgtagctg atggtcttcg agggcgtgcc gggatatgtc
     6481 acggtcaaga gacggcctaa gccgtcatag gtgctttgcg tcgtataggt ggtgccatcc
     6541 agcgttctgt cgcttcgcaa cacgcgcccc atactgtcgt actggagcac aactgtcccc
     6601 gaggcatccc aaatctgttg gagccgcccc ttacgattgt tcgttgtgcc gtcataggtg
     6661 tagcgaacgt cacccgaccc aagcgccttt tgggccacaa agtctttttg cacctgccgg
     6721 ttgagcgcgt cataccgaaa ccatgtcacc tggccctttg catcggtctg gtcagtgaga
     6781 ttgccgttcg catcatagcc gtagatccaa tagcccatat ccggatcacg catgttcttt
     6841 tttcggctct gctcatcata ggcgattgtg gtgacgttgc ctttggcatc ggttaccgcg
     6901 atgagattgt ctcggctgtc atactggtag acggtcgtcg catagtgaga tccgacctct
     6961 gtcgtgcagg tgctgaaggt gccttgatat tcatccacgc gaacggtccg cccataggca
     7021 tccttcgtca cgcgcgtgcg atgatcggcc gcatcgatgg tgacggtcac ccaatcgttg
     7081 aaacagacca cacttctggt gccgtccggg ctatccaggc gaatgacacg gcccaacgca
     7141 tcgtgactgc tcgtgctcca gcggcctgcg actgtctcaa ttgtcttaaa gtatggcagg
     7201 cttttttgac gcactgcgcc acggctatca tactgtactt cggtcacgat cacttttccg
     7261 tctggcccgg tcgcttcctt tcgaatggat ctccccagcc cgtcaaaaaa gctccaactg
     7321 gaaagcgggg tgacaattcc tgcccccgag gtcgtactca agacatgctg tgtgcccaca
     7381 ccgaagccag taccataatt gtaggccaca ctcgtgatgc caccgtcagg tgccgtaact
     7441 ttggtctttc ggcccaaagc atcatattcc atcaccgtga cttgactatt ggggtccgtc
     7501 acgctcttga tctgaccgta gagcccttta tccaccacct gcccatccac tccgtaatac
     7561 tgagtggtcg tcacatgtcc taacggattc gtggtcattt tggggaaggt cttcgtgctg
     7621 tcgtacgtta ccgtgctcac attgccattt gcatcacgag tacaggtgat gttcccccac
     7681 acatcgtatt cggctccggt gacagagagc cagttagtgg ctttggtaag gtgccccttg
     7741 gtgggtgtcg tggacccgtt tggcgaggcg cacccacctg tgccgtcata aaagaacgtt
     7801 ttgtgcgcca cgagattgac tgagactgta ctaatgctgt tgtagtgcga ttctcgcgtg
     7861 ggaagaccta caatccagtc tgcgacattg tccgagtagg tgcgatatat cgcgcgatca
     7921 tcgttggggt tcgccagatc gccgtagtgg gtttccaagg acaggtttcc atactggtga
     7981 tcgtactcga acgtcgtgcg cgtgtgagtc gcacatgtac cactttcaca aaggtaggtg
     8041 tcgatctgcg attgcggggt gtaccagggt gccgctagat cattatcagc cacatatgca
     8101 gtcgtggttt ccgagtagag tttgcctgtc gcatctgtga ccttggtacg atagggcatg
     8161 cctttggtgt agccattcgg cacgttagga ttgtttgagt ccaccccgag atcattgcct
     8221 tgatggaacc aggtttcagt aattgcttgc tcgccattcg gccccgccgg cgcagtgacc
     8281 ttcacatggt tgaaacctcg aaattcgcgt tcagcaaagt gatggtgccc accactataa
     8341 tcataatttg ctgtgcctga gatgcccgca cacgccgttc cattccagtt gtcgcagctc
     8401 acaatggagc tcacggtttg gattgaatag gggagttgcg tgttggtgta ccgggtggag
     8461 ggcttgtagg taatggctcg acgtccaccg actccgttcg agacggataa cagttgacca
     8521 gctaccatcg tgctatgatt cccgctcttc gcaacccaat agttaccgtt tgtgcgatct
     8581 tcgcacagaa agtccgtctt accatccccg ttgaaatccc cgtatctggt gaacaggccc
     8641 gtagctaatc cacacgcacc tgacgctgga tccccgtttc caagccacgt acccgtgtac
     8701 cctgcgccat ttccaaatct cgtgccatcg ctgagcgcca cgtaataggt acctgtgttt
     8761 ctattttcac agagaagatc cgccttcccg tcgccgttga aatcaccata ctgtggagtg
     8821 acatttgttg cagccacgca ggcgccattg gacgcattgc catttcccat ccattcctgc
     8881 gtatatccac ctccgctccc gaacttcgtg ccgtcactca aggccacata gtaggttccg
     8941 gagtttcgct cttcacagag gaggtcaatt tttccatcgc cgttgaagtc cccatattgt
     9001 gttgtgatat tcgctcccgc aatgcaggcc cctgacgcaa tatttccatt tccgagccaa
     9061 gtgcctgtcc accctgcacc attgccaaat tttgttccat cactcaaccc tacatagtag
     9121 gtgccagtat tccggtcctc acacaacaaa tccattttcc cgtcgccatt aaagtccccg
     9181 tatcgtgtcg taacattgag tgccggacca caagcgccat tcgcggcatt gccattgcct
     9241 aaccaagtgc ctgtccaccc gccaccgctg ccaaacttcg tgccatcgct caacgccatg
     9301 tagtaagttc cagtgttccg gtcctcgcat aacaaatccg ttttcccatc gccattaaag
     9361 tccgcatacc gcgtgtttaa tctggctgcc caaattgaat tcgcggggtt cggaaatgcg
     9421 caggctcccc ccgcataact gccattgcct aaccatatgc ccgtatagcc cgctccattc
     9481 ccaaatttgg tcccatcgct gagagccaca taaaacgttc cggtgtaacg atcctcacac
     9541 aagaggtcgg tttttccatc tccattaaaa tccgcatact gtgttcgaat tccatcattg
     9601 tatggaggtg cggcgggatg agcaggatcc cggtattgaa atggaggcag tccacaagcc
     9661 ccgctggcgg catttccgtt acctagccac gttcctgtcc agcctcctcc atcattgaat
     9721 agttctccac tgctcaatgc tacgtaatag gtgcctgtgt atcggtcctc acagagaatg
     9781 tcagttttgc cgtccccgtt gaaatctcca tagcgcgtat tgacgaggag cgcgttcacg
     9841 ttcgagcacg cccccgcccc aagccacagc ccggtatatc ccccctcggg cccaaatgtg
     9901 gctggtcccg ctgaccattc catggaagtg gacggtaacc gtgtcgccgt ggcttcattg
     9961 gtaatcgcgc ccgtgccatt aatcgtggca tcgctgccaa actgagtcac gctcgtgagc
    10021 accgaactag gaatgctcga tgtctggccg tacgtcatct tatacgcacg cacactgttt
    10081 cctgaggctt gcacctcaat ggttttgagc cgcttgacag tcttcaccac gtagttggtc
    10141 gtaaacatcg aaggcgcatc gcttcggtcc tcaagataaa acttcacgac attcgtgggt
    10201 gccgtcgtcc cgttgccagc gtactcaatc cgagagagat agacctgtcc ttggtcagac
    10261 aaataggtgg ctaccatata gttgcggtct cgatcttcaa tacgatccag cagccacttg
    10321 gatattcgtg agggattggc cgaatccgcc atccgggacg cggccgtttg gccgaagaaa
    10381 taccgcactc ccttcttgtc cgtcgcctca aagtatgctt gtccatcccc cgcactgagc
    10441 ttacggaacc tcacaaaacc gcgctctatc ttcgcacgat attcgttgct ctgtgctggc
    10501 gctggagcct gcacgagctc ggaattggca ccgctcattc gcgccacaaa gtcattcgcg
    10561 gaatagttga ggccatagcg cacttgtcgc tccacagcac cgatctctag cttccatccc
    10621 atccctaccc agccgttgcc attccccgac atatagacca gactgaggtt tggttggatg
    10681 ccattcctcc ctggagggac ttcaatggga atgctcatcg tggctgcgcc agtgaaggga
    10741 tccgcgtggg cctcgccacc aacattccca atgggcattt ccggctgctg aagcatttct
    10801 atggtacttg cctcttccgc acgcacctcg gtctctgcac tgaacgccat cagtagtccg
    10861 gccaaaaccg tgccttgacc ttcccccgat ttcacagaca cctgtataag gaggaatgtg
    10921 atgagatcg
//
LOCUS       DAORRQ010000095        10870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_277_length_10870_cov_18.928433, whole genome shotgun sequence.
ACCESSION   DAORRQ010000095 DAORRQ010000000
VERSION     DAORRQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_277_length_10870_cov_18.928433"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /gene="der"
                     /locus_tag="PK224_18810"
     CDS             <1..1344
                     /gene="der"
                     /locus_tag="PK224_18810"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRB17856.1"
                     /translation="APLFTLEGGRLPIIALIGRPNVGKSTLFNRILGTRAAIVDDVPG
                     VTRDRNYADSTYRNRRFRLVDTGGLDPTASDGMLSLIRQQSQLAIAEADILVLVLDAR
                     SGLTPADEEVVQTLRGSDKPVYYVINKIDTPKADPLVADFYRLGQEQLYPLSAEHGIG
                     VAELLDDLFSHMAEPLDEEATSDTPRIAIVGRPNVGKSTLVNSVLGEARVVVSDVPGT
                     TRDPVDSVATFKDRQYVLTDTAGIRRRGRVERGIEGYSVARSLRAIGRSDIAVLLLDA
                     EEGVTEQDTKIAGLVLKQGRACILIVNKWDLKTDDVHAREAYKQDLERRFPFLAWAPV
                     LFISALEQDFLRGLFALIDQVYFSFCKRVPTGPLNQFFQGLLEEHPLPVRKGKPAKAS
                     KSAFMTQVATRPPAFALFVGHPDNMTPAYLRFLENQIRKEYHFSGTPVRLLTRKK"
     gene            1572..1967
                     /locus_tag="PK224_18815"
     CDS             1572..1967
                     /locus_tag="PK224_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17857.1"
                     /translation="MFIGQPDLAAPAAPTSSVETGLIVLSMDGRILHTNDRAHEFARH
                     FTNEPRRLQAPATTPLRHPLMDLFHDVLANLEKHIAAEDWSQFEITRLARWGGGTVRL
                     RGFGIPDQARRQQSRVVLTVHYQSSPSPW"
     gene            2178..3188
                     /locus_tag="PK224_18820"
     CDS             2178..3188
                     /locus_tag="PK224_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17858.1"
                     /translation="MTYSTNSPQRLFFLSIRLGACLILAQPAWADTATPLIEPPPLAE
                     SGDPVTENNPEPAVAPAPVSPSVQISGAIWRSKPGIVFLQTPIGPLSLSSKSTLRDIK
                     TSQKITLWVHGTTSVIDIRERGVDKLVHRYVTGMPNFTSPEKNALTLWTPDGEQSFTL
                     GAFETKIAGRQDHQPLTIEVDGTGTIKGLHDVQFDLQVNQVPRTPSSLHLLLNGTVSK
                     LKSNYVFLKTPLGIVTVSGKTGVRNAKVGQEMSVWIQDRHVAIDLYQDGLTSPSRRFL
                     TGPLAYGSDAHDTLTMQGPEGAQSIPLTQRPAALATLKEGLPITVELNQQGELVDIRR
                     VN"
     gene            3266..3898
                     /locus_tag="PK224_18825"
     CDS             3266..3898
                     /locus_tag="PK224_18825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB17859.1"
                     /translation="MASRKNSRPEIPDGRGVSPSTSAPFDQLYRDHVDVMYRFAYRLC
                     GEAEAAKDLVQETFLNAYRAYDRFRGDAQVSTWLYAIASHACQRMRRKRKGEPERELS
                     LTEFVPTSEGEFALQIPMEGLSPQDALENKELRQILDRAIAKLPRKYRMVLVLRDMEG
                     LSAKEVGSILGLNERAIKSRLHRARLFVRKELSGKGLATEFHPDITTGHR"
     gene            3928..4227
                     /locus_tag="PK224_18830"
     CDS             3928..4227
                     /locus_tag="PK224_18830"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRB17860.1"
                     /translation="MADRVRTYPGTRKPTLHQGHGKRNCVKVLERLSAYLDDELSGDV
                     CQEIRAHLGDCPNCEVFLDSLRQTVQLCRNRPSPALSKNDRANLRRQILKAAATA"
     gene            4235..4720
                     /locus_tag="PK224_18835"
     CDS             4235..4720
                     /locus_tag="PK224_18835"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRB17861.1"
                     /translation="MTGPMITPAGEQPAEGARDESLSDQRLCPTQRYLPSMSDRLLLA
                     LLLTCGGTGVLLAQPSENFDHGQAIYREHCMECHGTTGKGDGVKAPFLSPRPGNLISA
                     ATSAKTDQELLRTIAQGKPRTAMPAWQNVLPAEDQQAVLQYIRSLVRFARPLTPPPPG
                     P"
     gene            4808..6529
                     /locus_tag="PK224_18840"
     CDS             4808..6529
                     /locus_tag="PK224_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17862.1"
                     /translation="MIRGNLFHRRQTIAIVTLALSLGLWAGTAESAPATGGKGHRPTA
                     SAKQPPAVQTAVRYAEALAHGDRVTAGQLDFACQYRYVVASPAKVKQFAPANDTSYDS
                     CWQTLTDAYAPMLKRSDIAMDIIWPSTGPLMFFGDDLPRAPASTFVADVLGTSPPGSG
                     LHVTVVGSRTIPSGSFRLGRAGKVLGVPTTLVQLSVQYQDPLTSPVTYAPGNVKWTNT
                     IKRPRLALKSVTTQWVVFTGLKQHGFPGDTAVFNLPVASQPEAPGMMADKIPFATETS
                     RALPESLAWWGPNDQPGTLTAAAARAAIFPDLRDRVALLNRVLLIDPKQPDALTVLTR
                     HLYSVLLREARNSHQVPIKDPALGIVVDEFYWNIYAQGARLDLSNGMEMGGLSQPTPA
                     DFLYRLIPALQTLVSVRPEQLDNRFRLGIAYRWNNDQLPMVETFESLVKDIPEHRRTP
                     KSEALLQLAWSRINKVSWNRILHDTETPRAYADAEAALAQAELPLDKFLAEYAMAYTM
                     IFLPNYGDKAKMLQHLTEAKRWFDEVPGKSDEVWRYFLHSELLKAVLDADPMFQPILA
                     TAAAPHA"
     gene            6626..7081
                     /locus_tag="PK224_18845"
     CDS             6626..7081
                     /locus_tag="PK224_18845"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRB17863.1"
                     /translation="MVIIMSTEESMPVTARTASASRCAIVATLIASHFSLTGCSIFGG
                     STQPLMVNSEPPGAQVLINGTAAGTTPLQYQVPRRGDLTVEVHKTGYQTQSRVTGRKL
                     SSTGIVDVIGGALFLLPLLGLIAPGAWEQDPSTLGITLEPDTHQPAQAP"
     gene            7133..8179
                     /locus_tag="PK224_18850"
     CDS             7133..8179
                     /locus_tag="PK224_18850"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRB17864.1"
                     /translation="MIDVHGYAALNAQSPLTPFRFSRREVGKQDVLIKIQYCGICHSD
                     VHQARDEWGGSLFPMVPGHEIVGTVEKVGASVKQFSVGQTVGVGCFVDSCRTCQACKK
                     GLEQYCEGHLAFTYNGKERDGVTPTYGGYSTKVVVDQRYIVRIPKRLRPEEAAPLLCA
                     GITTYSPLRHWGVGKNHRLAVVGLGGLGHMAVKIGKALGAHVTVLSHSDKKQADAKRL
                     GAQAYYSTAKPETLTQLTKRFDFILDTVSAPHDLNAYLELLKTDGTMILVGVPDKPAQ
                     LGAFPLIMRRRRLVGSLIGGIKETQEMLDFCGTHKFGADVEVIPIQQVNAAYDRLVRG
                     DVRYRFVIDMSSLT"
     gene            complement(8379..9344)
                     /locus_tag="PK224_18855"
     CDS             complement(8379..9344)
                     /locus_tag="PK224_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRB17865.1"
                     /translation="MTVRNIRFRQSVIRTVLGLAAVSGLAMQPVESAFAKVVEVSMTA
                     VETKLVIDGEGHTYDAWTFNGQFPGPVIRVQEGDEVVFSLTNPETNGRPHSMDFHAAE
                     TNFLEHYKEVRPGESLQYRFKAKWPGIFAYHCGASPMIQHIARGMMGAIIVDPKDPKA
                     LPKADREYVLVQSELFKDPDDVDGMFDRKYQHVVFNGSVFRYDPVHSKAGGDFLDAKP
                     GERVRFYFVNAGPNNVSAVHPIAEIWDDVWESGNPANHTRGVQTQLIPPAGAAILDMV
                     LDNNDGVYPIVTHSLTDALRGAIALLKVDKDARQLPLMPFVTPAK"
     gene            complement(9501..>10870)
                     /locus_tag="PK224_18860"
     CDS             complement(9501..>10870)
                     /locus_tag="PK224_18860"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRB17866.1"
                     /translation="AMSDLQRQLDTYIKDIRPRYEDMLGQAVEIPSISMDPRHAPDMR
                     RMAELAAQYLRAAGAETHIVETPGYPVVSGGWTVDPSYPTVTVYNHMDVQPAQEPEWK
                     QAPFAFQNDNGIYRGRGATDDKGPALAALFGARYAIEQGVPINVRFLWELEEENGSPS
                     FAAAIKNRAAIPRPDSVVISDTIWLSKNQPAMPYGLRGLLGARLLLRTGSKDAHSGVT
                     GGAARNPLAELMDIVHACVDAKTGKVKIPGFYEDVVEPTKAEIKSFLQSGFQVSKFKQ
                     AYGFKTLRTQDPAEVMRRIWAAPTFEVHGLVGGYHGPGVKTVVPGHGELKVSMRLVPN
                     QTPEKAFALLKKHVAKLNPEVTVEREGMLHPFRGVFDGPYVDAVKRAVKAGFGKEPAF
                     IREGGSIGAVVTMQKAWKVPILFMGLSLPEHGYHAPNEYFDWGQASGGMKAFVHYFAE
                     LAAKR"
BASE COUNT         2237 a         3569 c         2956 g         2108 t
ORIGIN      
        1 gccccgctct tcacccttga gggcggccgg ttgcccatta tcgccctcat cgggcggccc
       61 aatgtcggga aatcgacgct gttcaaccgt attctcggca cgcgggcggc catcgtggac
      121 gatgtgccgg gtgtgacgag ggatcgcaac tacgccgatt cgacctatcg caatcgccgc
      181 ttccgcctgg tggatacggg agggctggac cccacagcca gcgacggcat gttgtccctg
      241 atccggcaac aatcccaact cgccatcgcc gaggcggata tcctggtcct ggttctggat
      301 gcccgctcag gcctgacgcc ggccgacgaa gaagtcgtac aaaccctgcg cggctccgat
      361 aaacccgtct actacgtgat caacaaaatc gataccccga aggccgatcc gctggtcgcc
      421 gatttctatc gcctcgggca ggagcagctg tatcccctgt cggccgaaca tggcatcggc
      481 gtcgccgagt tgctggacga tctcttctcg cacatggcag agcccctcga cgaagaagcc
      541 acgagcgaca cgccccgcat cgccatcgtc ggacgtccga atgtcggcaa atccaccttg
      601 gtcaactcag tcctcggcga agcgcgcgtc gtcgtcagcg atgtgccggg cacgacgcgt
      661 gatcccgtcg actcggtcgc gacattcaaa gaccggcagt acgtgctgac cgatacggcc
      721 ggcatccgcc gccgtggtcg tgtggaacga ggcatcgagg gctacagcgt cgcccgatcc
      781 ctgcgcgcca tcggccgatc agacatcgcc gtgttgctgc tggatgcgga agaaggcgtc
      841 accgagcagg acactaaaat tgcgggcctc gtcctcaagc aaggacgcgc ctgcattctc
      901 atcgtcaaca agtgggatct caagaccgac gacgtgcacg cgcgcgaggc ctacaaacaa
      961 gatcttgaac gtcgcttccc gttcctcgcc tgggcaccgg tgctgttcat ttcggcgctg
     1021 gagcaggatt ttctgcgcgg cttgttcgcc ttgatcgacc aggtctattt ctcattctgc
     1081 aaacgggtcc ccaccggccc cctgaatcaa ttctttcagg gtctgctgga agaacatccg
     1141 ttgccggtca ggaaaggaaa gccggccaaa gccagcaaat cggcattcat gacgcaagtt
     1201 gcgacacggc ctcccgcatt cgccctcttt gtgggacatc ccgacaatat gaccccggcc
     1261 tacctccgtt tcctagaaaa ccagatcagg aaggaatatc atttctccgg cacgcccgtt
     1321 cgactgttga ccagaaaaaa atgaggcagc cttgccggag cgcgacgcat tcgacacaat
     1381 cgacgacatt ttccttcgcc cccttcgttt caccgctcac gattccttga acatttcgta
     1441 gatttcctcc cttctctcag ctcaacgcaa tggcccgcat cttgcttttc cattcagcat
     1501 tcgaatggca gcgacgaccg tcctcgctga ccgcatgagg gacatgccat agcagagagg
     1561 agcctatctc tatgttcatc gggcaaccag acctcgccgc ccccgctgca ccgacctcgt
     1621 cggtcgagac cggcctcatc gtcctttcca tggacggtcg cattctccat accaacgacc
     1681 gggcacacga gttcgcgcgc catttcacga acgagccgcg ccggctgcag gcccccgcga
     1741 ccacaccact ccgtcatccg ttgatggacc tgtttcatga cgtcctggcc aatttagaga
     1801 aacacatcgc ggcagaagac tggagccaat tcgaaatcac ccgcctcgcc cgatggggcg
     1861 gcggcacggt gcgtctccgg ggattcggca ttccagacca agccagacgc cagcagtcac
     1921 gcgtcgtcct gacagtccat taccagtcga gcccctcccc ttggtaagat tcagcctgcg
     1981 ccatgcacgg cccaggcgat tcgcaggtcg cgaacggctt tcaacttgac tcatacggat
     2041 cagcatgtta ttcgcacagg agacgttgtt gtacgccacg cgcgacaagg caggaaccgg
     2101 ccccctccag ctacgggagg tttctcctcc accctttcgg cgtcatacca ttccggtcaa
     2161 gacggtctat aaccactatg acttattcta caaattcccc tcagcggctt ttctttctct
     2221 cgatccggtt gggcgcttgt cttatcctgg cacaaccagc gtgggcagac accgccacac
     2281 ccctcattga accgcccccg ctcgctgagt ccggcgatcc ggtcacggaa aacaaccctg
     2341 aaccggcggt ggcccccgcg cccgtgtccc cttcggttca gatttccggc gcgatctggc
     2401 gctcgaaacc cggcatcgtg tttttgcaga ccccgatcgg gccactctcc ctgagttcga
     2461 aatccaccct tcgcgacatt aagacttcac aaaagatcac tctgtgggtc catggtacga
     2521 ccagtgtcat cgacatccgc gagaggggtg tcgacaaact cgtccatcgg tatgtcaccg
     2581 gcatgccgaa ctttacctcc cctgagaaaa acgccctcac actctggact ccggatggcg
     2641 aacaatcctt cacactcggc gcatttgaaa ccaaaatcgc cggacgccag gatcaccaac
     2701 cattgaccat cgaggtcgat gggaccggaa ccatcaaagg tctgcacgat gtgcagttcg
     2761 acctccaggt caatcaggtt ccccgcacac cttcgtcgct gcatctcttg ttgaacggca
     2821 cggtctcgaa actgaagtcg aactatgtgt tcctgaaaac ccccctgggc atcgtgacag
     2881 tcagcggaaa gaccggtgta cggaacgcca aggtcgggca ggagatgtcg gtgtggatcc
     2941 aggatcgaca tgtggccatc gatctgtatc aagacggtct tacaagtcca tcgcgccgat
     3001 tcctcaccgg gccgctggcc tacggttccg acgcgcatga caccctcacc atgcagggcc
     3061 ccgagggagc ccagtccatt cccctcacgc aacggccggc tgctctcgca accctaaagg
     3121 aagggctccc gatcaccgtc gaacttaacc agcagggtga actcgtcgat atccgacggg
     3181 tcaattgacg cccctctttc ggcgcctcga catccgggcc gcttcttgac agtgcccggc
     3241 gccccccgta gactccctga cgcgtatggc atcacggaag aacagtcggc cggaaatccc
     3301 cgacgggcgc ggcgtctcac cctccacctc cgctccattc gatcagctgt accgtgatca
     3361 cgtcgacgtg atgtaccgct ttgcctatcg cctgtgcggt gaagccgagg cggccaaaga
     3421 tctggtccag gaaacgtttc tgaatgccta tcgcgcctac gaccgatttc gtggcgacgc
     3481 gcaggtctcg acttggctct acgccatcgc ctcccatgcc tgccagcgta tgcggcgcaa
     3541 acgcaaaggc gaaccggagc gcgagttatc cctgaccgaa ttcgttccga catcggaagg
     3601 cgagtttgcc ctgcagatcc cgatggaggg cctcagccct caagacgcgc ttgaaaataa
     3661 agaactgcgg cagatactcg atcgcgccat cgcaaagttg ccgcgcaaat accggatggt
     3721 gctggtgctc cgcgacatgg aaggattgag cgccaaagaa gtggggagta ttctggggct
     3781 caacgaacgg gccatcaaat cgaggctgca tcgagcccgc ctctttgtcc gcaaggaact
     3841 cagcggcaaa ggattggcga cagaatttca cccggacatt accaccgggc accgatagtt
     3901 tggtatagtc taagaagaag caccgccatg gctgatcgcg tacgcacata tccaggcacc
     3961 cgtaagccca ccctccacca gggtcacgga aaacgaaatt gcgtcaaagt gctcgaacgg
     4021 ctgtctgcct atctggatga cgagctttcc ggtgacgtct gtcaggaaat tcgcgcccac
     4081 ctcggcgatt gtccgaattg cgaagtgttt ctcgactcct tgcgccaaac cgtgcagctc
     4141 tgccgcaacc gtccttcccc ggcactctcc aagaacgatc gtgcgaacct ccgccgacag
     4201 attttgaagg ctgcggcaac ggcgtaaccc aatcgtgact ggcccgatga tcacgccggc
     4261 cggggaacaa ccggcagagg gggcgagaga cgagtcgttg agtgatcagc ggctgtgccc
     4321 gacgcaacga tatttgcctt cgatgtcaga ccgcctcctg ctcgcccttc tattgacctg
     4381 cggtggaacc ggcgtcctgc tcgcgcaacc ttcagaaaat ttcgaccacg ggcaagccat
     4441 ctatcgtgag cattgcatgg agtgccatgg cacaaccggc aagggcgacg gggtcaaggc
     4501 cccgtttctc tcccctcgac cgggcaatct catctcggcg gcgacctctg ccaaaacgga
     4561 tcaagagttg ctccgcacga tcgcccaagg aaaaccccgc acggccatgc cggcctggca
     4621 gaacgtgctg cccgccgagg atcagcaggc cgtcctccaa tacatccgct ccttggtccg
     4681 ctttgcccgg ccactcactc cccctccacc gggtccgtaa tcttgcatcg ccccagcacg
     4741 cccctttcac ctgaaaagag ccccgtggta cagtgacatt cttttactgc caccggaggt
     4801 cgttcgcatg attcgcggga acctgttcca tcgacgacag accattgcca tcgtgaccct
     4861 cgcgctctcg ctcggtctct gggccgggac agccgagtcg gcaccggcta ccggcgggaa
     4921 gggacatcgc ccaaccgcct ccgctaagca accaccggcg gtccaaaccg ccgtccgata
     4981 tgccgaagcc cttgcgcacg gcgatcgcgt caccgccgga caattggact ttgcctgcca
     5041 ataccgatac gtcgttgcct ctccggcgaa ggtgaaacaa ttcgcgccgg ccaatgacac
     5101 gtcctacgat tcctgctggc aaaccctgac cgatgcctat gcgcccatgc tcaagcggtc
     5161 ggacattgcc atggatatca tttggccgag caccggcccg ctgatgtttt tcggtgacga
     5221 tctcccgagg gcgccggcct cgaccttcgt ggccgacgtg ctgggcacct caccccccgg
     5281 gagcggcctt cacgtgaccg tcgtcggcag ccgcaccatt ccgtccggtt cgttccgcct
     5341 gggacgcgca ggaaaggtgc tcggagttcc aacgacgctg gtccaactgt ctgtgcagta
     5401 tcaggatccc ctgacctcgc cggtcacgta tgcccccggc aacgtcaagt ggacgaacac
     5461 gatcaagcgc ccgagactgg cattgaaatc cgtgactacc cagtgggtcg tcttcaccgg
     5521 gctgaagcag catggatttc ccggcgatac cgccgtattc aatctgccgg tcgcctcgca
     5581 gccggaagcc ccgggcatga tggcagacaa gattccgttt gccaccgaaa caagccgcgc
     5641 actgcctgag tccctggcct ggtggggacc gaacgatcaa cccggcacgt tgacagccgc
     5701 tgcggcacgc gccgccatct ttcccgatct gcgcgaccgg gtggccttac tcaaccgcgt
     5761 cttgctcatc gacccgaagc agccggatgc cctcacggtc ttgacccgcc acctgtatag
     5821 cgtgttgctg cgtgaagccc gcaatagcca tcaggtgccc ataaaggatc ctgccctggg
     5881 cattgtggtg gacgaatttt attggaacat ttatgcgcag ggagcccgct tggatctctc
     5941 gaacgggatg gaaatgggcg ggctctcaca gccgaccccg gcagactttc tctatcgtct
     6001 catcccggcg cttcaaacgc tcgtttcggt cagaccggaa caactcgaca accggtttcg
     6061 acttggaatc gcctaccgat ggaacaacga ccagctcccc atggtcgaga ccttcgaatc
     6121 cctggtaaag gatattccag agcaccggag aacgcccaag tccgaggccc tgctccagct
     6181 ggcctggtcc cgcatcaaca aagtgtcctg gaaccggatc cttcacgata cggagacgcc
     6241 tcgggcctat gccgatgccg aggccgccct ggcacaggcc gaactgccac tcgataagtt
     6301 tctggccgaa tatgcgatgg cctacaccat gatctttttg ccgaactacg gggacaaggc
     6361 caaaatgctt cagcacctga ccgaagccaa gcggtggttc gacgaggtac ccggcaagtc
     6421 ggatgaggtg tggcggtact tcctgcactc ggaactgctc aaagccgtgc tggacgccga
     6481 tccgatgttc caaccgatcc tggcaaccgc cgccgccccc cacgcctgac aacacacgga
     6541 gcggggcggt ggagtcagaa gtactccacc gccccgcgca ctccagcctc cgccgccttg
     6601 tcagcccgca tacgctgtgc taaccatggt gatcatcatg tccacggagg agtccatgcc
     6661 cgtcacggcc agaaccgcca gcgcaagtcg ttgtgccatc gtcgcaaccc tgatcgcgtc
     6721 tcatttcagc ctgaccggct gctcaatttt cggtggaagc acgcaaccac tgatggtcaa
     6781 ttcagagcca cccggcgcac aggtcctcat caatggcacc gcagccggaa cgacgccgtt
     6841 gcagtatcag gtcccccgaa gaggtgacct caccgtcgaa gtgcacaaaa cgggatacca
     6901 gactcagtcc cgagtgacgg gccggaaact cagtagcacc gggatcgtcg atgtcatcgg
     6961 gggcgcattg ttccttcttc cgttgctcgg actgattgcg ccgggcgcgt gggaacagga
     7021 cccctccact ctcggcatca ccctcgaacc cgacacacat caaccggccc aagcgcctta
     7081 gcgacgcctg catcacagca caaacacgcc attttttcag gagcagacca tcatgatcga
     7141 cgtccacggg tatgctgcac tcaacgctca atccccgctc acgccgttca gattttcacg
     7201 ccgcgaagtg ggcaaacagg atgtattgat caagatccag tactgcggga tttgccattc
     7261 ggatgtgcac caggcgcggg atgaatgggg cggatcactc tttccgatgg tgcccggcca
     7321 tgaaatcgtc ggcacggtgg aaaaagtcgg ggcctccgtc aaacaattct ccgtcggtca
     7381 aaccgtgggg gtcggctgct tcgtcgattc ctgccggaca tgccaggcat gcaagaaggg
     7441 cctggagcaa tattgcgaag gccacctcgc cttcacctat aacggtaaag agcgggacgg
     7501 cgtcacaccc acctacggcg gatactccac caaggtcgtc gtcgatcaac gttatattgt
     7561 gcggattccc aaacggttgc gccctgaaga agcggcgccg ctcttgtgcg ccggcatcac
     7621 cacctactcc ccgttgcggc actggggggt gggcaagaac catcggctcg ctgtggtcgg
     7681 gttaggcggc ctcgggcaca tggccgtcaa aatcggcaag gcgctcggcg cgcatgtcac
     7741 cgtcctgagc cactccgaca agaaacaagc cgatgcgaag cggctgggcg cacaggccta
     7801 ctactctacc gcaaaacccg aaacgttgac ccaactcacc aaacggttcg acttcatcct
     7861 ggataccgtc tcagcgcccc atgatctgaa tgcctatctg gaactgctga agaccgacgg
     7921 caccatgatt ctggtcgggg ttccggataa gcccgcgcag ctcggcgcct ttcccctgat
     7981 catgcgccga cggcgactgg tcggatcgtt gatcggcggg atcaaggaga ctcaagagat
     8041 gctcgacttc tgcggcacac acaagttcgg cgcagatgtg gaagtcattc ccatccagca
     8101 ggtgaatgcc gcctacgacc gtcttgttcg gggcgacgtg cgttatcgct tcgtgatcga
     8161 tatgagttcc ctgacgtaga cgtagcgcct ggtatccgac gctctcagag atgagtccca
     8221 accaggcacg ggctcgcctt ctatgcgcac ccactcaaat cgggtggggg cagaacctgt
     8281 ggtcagcgaa tgcagaaact gagagcggct cacgaaagtg ggagagtcga acgaagcggc
     8341 aggccgggcg gccgcaaggc cgcccggcga atccaccact acttggcagg agtgacaaac
     8401 ggcatgagcg gcaactgccg cgcatccttg tccaccttca acagggcaat ggcaccgcga
     8461 agggcgtcgg tcaaggagtg ggtgacgatc gggtaaaccc cgtcgttgtt gtccagcacc
     8521 atatcgagga tcgccgcgcc cgccggcgga atcagctgcg tctgcacgcc gcgcgtatgg
     8581 ttggcgggat tcccgctctc ccacacatca tcccagattt cagcgatcgg atggaccgcc
     8641 gacacgttgt tcggtccggc attcacaaaa tagaaccgga cccgttcccc cggcttggcg
     8701 tccaggaagt cgcctcccgc cttgctgtgc acaggatcgt accggaagac ggagccgtta
     8761 aacaccacat gctgatactt ccggtcgaac atcccgtcaa catcgtcggg atccttgaac
     8821 agttcgctct gcaccagcac atactcgcga tcggctttcg gcagcgcctt gggatccttg
     8881 ggatcgacga taatggcgcc catcatgccg cgcgcaatat gctgaatcat gggagaggca
     8941 ccgcagtgat aggcaaagat gcccggccat ttggccttga aacgatattg cagcgactcg
     9001 ccgggacgca cttccttgta gtgctccagg aagttggtct ccgccgcgtg aaaatccatg
     9061 gaatgggggc gaccgttggt ctcgggattc gtcagtgaaa agacaacctc gtcaccttcc
     9121 tgcacacgaa tcaccggacc gggaaactga ccgttaaaag tccaggcgtc atatgtatgt
     9181 ccctccccgt cgatgacgag tttcgtttct acggcggtca tggaaacttc aacgactttc
     9241 gcgaacgcgg attcgaccgg ctgcatggcc aggcccgaga ctgcggccaa tcccaagacc
     9301 gtgcgaatca cagattgacg aaagcggatg ttacgtactg tcataatgct gcggctcctc
     9361 ctgttgaaag tgaatgggta tgggttcatt cagaacccca ggcaggatac cgaatcgtca
     9421 gctcaaacca atatgacaat tgtcatgttt ttaaattacc tccggactgg taggcgttct
     9481 cctacagctc agggtaccgc ttagcgcttc gcggccagtt ccgcaaaata atgcacaaac
     9541 gccttcatcc cgcctgaggc ctggccccag tcgaaatatt cgttcggcgc atggtagccg
     9601 tgctcaggca ggctcaagcc catgaacaga atcggaactt tccaggcttt ctgcatcgtc
     9661 accaccgcgc cgatcgagcc accttcgcga ataaaggccg gttcttttcc gaatccggcc
     9721 ttcacggcgc gtttcaccgc atcgacgtat ggcccgtcaa acacgcctct gaacggatgc
     9781 aacatcccct cgcgctcgac cgtcacctcc ggattcaact tggcgacatg tttcttgagc
     9841 agcgcaaagg ctttctccgg agtctgattc ggtacgagcc gcatgctgac cttcagctcc
     9901 ccatggccag ggaccaccgt tttcaccccc ggcccatggt agccgccgac gagtccgtgc
     9961 acctcaaacg tcggcgcggc ccaaatacgg cgcatgacct ctgccggatc ctgggtgcga
    10021 agcgtcttga atccataggc ctgcttgaac ttgctcactt gaaatccaga ctgcaggaaa
    10081 ctcttgatct ctgcctttgt cggctcgacc acatcttcgt agaagcccgg aatcttcacc
    10141 ttaccggtct tggcatccac gcaggcatgg acgatgtcca tcaactcggc cagcgggttt
    10201 cgcgccgcgc cgccggtcac gcccgaatgg gcgtccttgg agccggtacg caggaggagg
    10261 cgcgcgccca gcagcccccg cagtccatag ggcatcgcag gctgattctt cgacagccag
    10321 atcgtatccg aaatcaccac cgagtccgga cgtgggatcg ccgcacgatt cttgatcgct
    10381 gccgcaaaac ttggactgcc gttttcctct tccagttccc acaagaatcg cacgttgatg
    10441 ggcaccccct gctcgatcgc ataacgcgcg ccgaacaagg ccgccagcgc cgggcctttg
    10501 tcgtccgtcg cccctcgccc ccggtagata ccgttgtcgt tttgaaacgc aaagggggcc
    10561 tgtttccatt ccggctcctg ggccggctgc acatccatgt gattgtaaac ggtcacggtc
    10621 ggataactcg gatcgaccgt ccatccgcct gacacgaccg ggtagcccgg ggtctcgacg
    10681 atgtgcgtct ccgccccggc ggcccgcaga tattgcgccg ccagttccgc catccggcgc
    10741 atatccggcg catgtcgggg atccatgctg atagagggaa tttccactgc ctgcccgagc
    10801 atgtcttcgt agcgcgggcg aatgtcctta atgtaggtat ctaactggcg ctgcaagtcg
    10861 ctcatcgctg
//
LOCUS       DAORRQ010000096        10803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_281_length_10803_cov_24.828526, whole genome shotgun sequence.
ACCESSION   DAORRQ010000096 DAORRQ010000000
VERSION     DAORRQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_281_length_10803_cov_24.828526"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2314)
                     /locus_tag="PK224_18865"
     CDS             complement(<1..2314)
                     /locus_tag="PK224_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRB17867.1"
                     /translation="MDCHWFTRVTSRALSVIAGGALVCLPLAASAETQPHVDHAAPQQ
                     TAMSHQLPSWAEKLKGQTIVEDAMAGKPERSAMVEQQHQRIMEHLTHDPQAQQVNTGM
                     FNTQTMMHQYGAGGQDLLLMSDPRVEPVALSGGGKCPASAPVKQYNVSAINIEITLNQ
                     WLDFYPGYMYTLDENLDKVRAEETKNREARDKEGFDPGAVIPGVQAQWIQPLTIRGNQ
                     GDCVKIKLSNKLEGGEDVSLHIHGSSMVVSATGAAAATTNSDSIVQKDKSGEFEWYIH
                     PSTQEGVRQFHTFANDRELTVMGLFGSFVVEPRGSEYLEALGSGAPTPASSGWQVMIK
                     NGTGPDFREFVLYYHEVGDEAFRPVNKKGDFLPQRDPLTDAYRPGGRAINYRSEPFGI
                     NNMHVQHEYFGFEDESMGYSSYTFGDPSPTIPRSYMGDPAKFRLVHGGSEVFHSHHPH
                     GGTIRWPRSPRAIDDMNLWTTAGNGPVKYPVIRAKTDRVDVEVIGPSEALDLETECGS
                     GLCQQLAGDFLFHCHVAHHYVAGMWGYWRVYNTLQQGDMRNDVMPDLLELPDRKGRIK
                     GPITSDKLVGQTVDWFGKSFKIVEKGKSDWKSNPATITIKDWVEMQLPTMGKPGHKND
                     EKGQTVSYDATVLDWAWEGTRALSEPESTIDNPKYKSTHPGKRHPITFEPLTGKVAWP
                     HLTPHFGRRVMFSPNHVGAPWLEMIRRDANGEESVDQALPGENGNWSLCPENAGRKHY
                     NVHFIKLPITIAKKIGKEPPVIDPNGLIYVL"
     gene            2669..4483
                     /locus_tag="PK224_18870"
     CDS             2669..4483
                     /locus_tag="PK224_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRB17868.1"
                     /translation="MILKRWLVGLPLKTKEAAHERLSKRLALAVFSSDALSSVAYATE
                     EILLVLTLAGAAMVGYSIPLSLSIIGLLIILTMSYRQIIFEYPEGGGAYIVGKSNLGE
                     WPGLVAAAALMIDYVLTVAVSVAAGMAALISAVPALLPHREALCVAAILLVTVVNLRG
                     VRESGQFFAVPTYIFIATIAAMLGVGAVQILFGHAARVEPLPSLAPTEPLTLFLLLRA
                     FSSGCTALTGVEVISNGVSAFKKPEPKNAAFTMIGMAAILGTMFIGISAMAYYFGIVP
                     KEDETVVSQIARATFGTGPLYFLVQASTMVILILAANSSFNGFPRLASILARDSYMPH
                     QMSMMGDRLVFSNGVIILGVFSCLLIVLFNGDTHALIPLYAVGVFLSFTISQAGMVKR
                     WLVKKGPHWEKKLLVNGIGAVTTAIATLIIASTKFAHGAWIVIVLIPLLITFFRAIRS
                     HYKAVSEQVALSRGHRPPMPRRNIVVLPIGGVNRAVIRAVDYARSRSGDIRAVLVDVD
                     PEETARVEIQWAQWGCGVPLTVLPSPYRSVLSSLLDYLEQVLQKDQECWVTVVIPEIL
                     PARWWQNILHNQRAFMLKGALLFKDRVILTDVPYHLTR"
     gene            4624..5607
                     /locus_tag="PK224_18875"
     CDS             4624..5607
                     /locus_tag="PK224_18875"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRB17869.1"
                     /translation="MTSGQAVPVEVEAGREAGLVEVRYYWYPEQTEALVEQGEDRAGK
                     ASQGSMATEKYWQKDSITGAPVVALPALTSTVDRVPPYGGALPVPSDAIGRMRLLAIA
                     DISQGRLGRKTVFDEVFVTVQPAVDLLSIDFETEKPLQLGRTGQSSAYGHVDSLGKIF
                     ELPVVGEFADGVMRPLSSPSTGTKYSSGDDHILKVLPDGLLQIVGNGKTSLTVTNRGK
                     QATLDVRVEVNPEANEPPVADAGAAKTVKAGAKVRLNGLQSRDPEGEALFYAWSQVRG
                     SKVAMLDVNMAEPSFTAPYVSETRTFRFKLRVTDKKGADSVPAYVDVTVEP"
     gene            5996..6370
                     /locus_tag="PK224_18880"
     CDS             5996..6370
                     /locus_tag="PK224_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17870.1"
                     /translation="MKAVLECQKRLVSTMRLPFSETLASELKAQSEEWVTPEWCEQSL
                     QSDPDLGLMKDYKTGFREETLQARNSALRKASIEIDLLVDELRTQRPAETLTLSRELD
                     QKFKILLSPGRLKRISQTGLNN"
     gene            6376..7323
                     /locus_tag="PK224_18885"
     CDS             6376..7323
                     /locus_tag="PK224_18885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRB17871.1"
                     /translation="MNGQIAVLFADLDNFKALNTKYSEPRIDQSLLPDAMHLVEGLIR
                     MRGGAYKYGGDEYVLILPNHDAIEGAGFAEKVRKAFEQFEFRVDTDLVHITVSLGIAV
                     WPVHSTTYQDLLVKASEAKTQAKVQKNCFRMAEIIGGPGAPLPRSGLTLSAQHLAVYL
                     NRKSQHGLDHDPMIDPASLCNELSFTEDVLALAADELAEHGWIKLIKTMGMGKAGFST
                     LSPTALLFIETDQFIRGRDPKADARELAAQLIKQPEGSANLSELDKQLSWGPRRINPS
                     VSFLQLKEYVQASKTLGAHPYAVSWIRATEKTKRFALNV"
     gene            complement(7391..7786)
                     /locus_tag="PK224_18890"
     CDS             complement(7391..7786)
                     /locus_tag="PK224_18890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HRB17872.1"
                     /translation="MREFIFLGVEDVLFLHADTIGIDGGSPGVRAHGLLDAAVAMPRQ
                     QFGGKFLHGDLTAMAAAYHFHIAQNHPFIDGNKRVAVMAALVFLHVNGIEKLPAPEAL
                     EITTRQVAAGELSKDALTQWLRAQIGKRK"
     gene            complement(7790..7984)
                     /locus_tag="PK224_18895"
     CDS             complement(7790..7984)
                     /locus_tag="PK224_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17873.1"
                     /translation="MALVIEKPMMEALGITEETPLQVTVNGNALVVTPANVGVGPERM
                     TAITKELRKRYGPMLKRLAH"
     gene            complement(8465..10012)
                     /locus_tag="PK224_18900"
     CDS             complement(8465..10012)
                     /locus_tag="PK224_18900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HRB17874.1"
                     /translation="MTLLDARVLEQLISEFRRSMRGLLKEVCQDFERNYADAACTFGL
                     PIDWFRTLGQSFTSEDYSNGKVVGWIESLNDLLYFVDILVQVRQERSRGEIAAQLRAE
                     FREKFYEHGYADEIFPNGKPEPRLLLSRLTAFCQRLAREITQESVCLAPQLACAWVAG
                     QGKDAWLVPCDLNANVERVELPWVCAVGTAGLSYVAPAPVRSALKRAGGQGEFLIKPS
                     GIDLLVGDQDYALVAYGKKARWHWRRLEPLCLRESQYGALVLGPTLVYGKDKTPVAVR
                     PTRPEIAARMRRALSVIASAWPEGDRLLALLTSRVVPLKASGVVSFSYRHRPGLSAIN
                     CFDRDRLDLIDDLIHENSHHHLNLLLRKDAMYQHDHNQEIFYSPWRRSLRPLRGILHA
                     TFTFTMGAMLFERLSTWASGRAGTARWKRAGLSPRDLERARYRCLEEIDSVRYSLKDL
                     DLAGGHFKWLTRGGKQVVKQLADALAHIEQQMLTHEKDVSRSSFGPALRRHRAELARA
                     RELFSLV"
     gene            complement(10255..10482)
                     /locus_tag="PK224_18905"
     CDS             complement(10255..10482)
                     /locus_tag="PK224_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17875.1"
                     /translation="MTCTRCEGLMVTDDLIDLQESYHPMRLSGHRCLSCGNVVDTLIQ
                     RHRMVQNSHLHPVPVSAPEKPFLFDLIKLTA"
BASE COUNT         2334 a         3298 c         2910 g         2261 t
ORIGIN      
        1 gcaagacata gatcaagccg ttcggatcga tgaccggcgg ctccttgccg attttcttgg
       61 cgatggtgat cgggagcttg atgaagtgca cgttgtagtg tttccggccc gcattctccg
      121 ggcagagact ccagttaccg ttttcaccag gcagggcctg atcgacgctt tcctcgccgt
      181 tggcatcccg gcggatcatc tccaaccagg gcgcacccac atggttcggg gagaacatga
      241 cgcgtctccc gaaatgcggg gtgaggtgag gccaggcgac tttaccggtc aacggctcga
      301 atgtgatcgg gtgccgcttg cccggatggg tcgacttata ctttggattg tcgatcgtgc
      361 tttccggttc gctcaatgca cgggtgcctt cccaggccca atccaacacc gtggcatcgt
      421 aggacacggt ctgtcccttt tcatcgttct tgtgtcccgg cttgcccatc gtcgggagct
      481 gcatttcgac ccagtccttg atcgtgatcg tggccggatt ggacttccaa tcgctcttgc
      541 ccttctcgac gatcttgaag gacttgccga accaatccac agtctgaccg actaacttat
      601 ccgacgtgat cggacccttg atacgacctt tccgatcggg caactcaagc agatccggca
      661 tgacgtcgtt acgcatgtcg ccctgttgaa gggtgttgta tacccgccag tatccccaca
      721 tgcccgccac ataatggtgc gccacgtggc aatggaacag gaagtcgccg gccaactgct
      781 ggcacaggcc ggagccgcac tccgtctcca agtccaacgc ttccgacggc ccgatgactt
      841 cgacgtcgac acgatccgtc ttggcacgga tgaccggata cttgaccggt ccgtttccgg
      901 cggtcgtcca cagattcatg tcatcgatgg cgcgcggact gcgcggccac cggatggtgc
      961 cgccatgcgg atggtggcta tggaacactt ccgatccgcc atggaccagg cggaacttcg
     1021 ccgggtcgcc catgtacgag cgcgggatcg tgggagacgg atccccgaag gtgtacgagc
     1081 tgtagcccat cgactcgtct tcgaatccga agtactcgtg ctgcacgtgc atattgttga
     1141 ttccgaacgg ctcgctgcga tagttgatcg cacgaccgcc ggggcgatag gcgtcggtca
     1201 gcgggtcacg ctgaggaagg aagtcgcctt tcttattgac ggggcggaag gcttcgtcgc
     1261 cgacctcgtg atagtacagg acgaattcac ggaagtccgg accggtcccg ttcttgatca
     1321 tgacctgcca gccgctcgag gcgggcgtgg gcgccccgct ccccaacgct tccaggtatt
     1381 cggatccgcg cggctcgacg acaaaggacc cgaagaggcc catcacggtc aactcgcggt
     1441 cattggcgaa cgtatggaat tgccggacac cttcctgggt gctcggatga atgtaccatt
     1501 cgaactctcc gctcttatcc ttctggacga tgctgtcgga gttcgtggtg gcggctgcgg
     1561 caccggtggc gctcaccacc atgctggagc catggatgtg aaggctgacg tcctcgccgc
     1621 cttccaactt gttgctcaac ttgatcttga cgcaatcccc ctggttgccg cgaatcgtca
     1681 gcggctgaat ccactgagct tgcacaccgg gaatcaccgc gccaggatca aagccttcct
     1741 tgtcgcgggc ttcccgattc ttcgtttctt ccgcccgaac cttgtcgaga ttttcatcca
     1801 gcgtgtacat gtagccggga taaaaatcga gccactggtt cagcgtgatc tcgatgttga
     1861 tcgccgacac gttgtattgc ttgaccggtg cggaggccgg gcacttcccg cctcccgaca
     1921 atgccactgg ctccactcgt gggtcggaca tcaacaggag atcctgaccg cctgccccat
     1981 attggtgcat catggtctgg gtattgaaca tacccgtatt gacctgctgc gcctgcggat
     2041 catgagtcaa atgctccatg atccgttggt gctgctgctc aaccatcgca gagcgctccg
     2101 gctttccggc catcgcatct tcgacaatcg tctgcccctt caacttctcc gcccaagacg
     2161 ggagttgatg ggacatcgct gtttgttgcg gcgccgcatg gtccacatgc ggctgagttt
     2221 cagccgatgc ggctagcgga agacacacaa gcgcaccacc agcgataacg ctgagggcgc
     2281 gcgatgtgac acgagtgaac caatgacaat ccatggaccg gcctccttgg atagagtttg
     2341 gaaagaaaca gatgaaaagt tatgagcgct tacgatcaga acatgacatc ctcaatactg
     2401 gagatacgca ttctgcctgt aatcctgcgg catcacgcac gcagggcact cctttagaaa
     2461 acaagtgccg gagattactg aagtcacccg cccctgtcaa ccccctcacc tgctcgcaca
     2521 gcatgatgcc aagcgatcgt acgctcaact tgatcgactt caatacacgt tctccccaga
     2581 gcaaactctg aggttgccgc gccttaccat caccaacaac atttcccggt ccattgtctc
     2641 tcttccgcaa cttggggcat aatgacccat gatcttgaaa cgctggcttg tcggcctgcc
     2701 actcaagacg aaggaagccg ctcacgaacg cctttctaag cgactcgccc tagccgtctt
     2761 ttcctccgat gccttgtcct cggtggccta tgccacggag gaaattctgc tcgtcctgac
     2821 gctggccggc gcagccatgg tcggctattc catcccgctc agcctctcca tcatcggtct
     2881 gctgattatc ctgaccatgt cctaccggca gattattttt gaatatccgg agggtggcgg
     2941 cgcctacatc gtgggcaagt cgaacctcgg cgaatggccg ggactggtcg cggccgcggc
     3001 cttaatgatc gactatgtgt tgaccgtcgc cgtcagcgtc gcggccggaa tggccgcgct
     3061 catctcagcc gttccggcgc tccttcccca tcgtgaagct ctctgcgtcg ccgcaatcct
     3121 tctggtgacc gtcgtcaact tgcgaggtgt ccgcgagtcg gggcagttct tcgccgtgcc
     3181 gacctatatc ttcatcgcaa ccatcgcggc catgctcggc gtcggcgccg tccagattct
     3241 gttcggccat gcggcacgtg tcgagccgct ccccagcctg gcgcccacgg aacctttgac
     3301 gctgtttctc cttcttcgcg cattttcttc cggctgtacc gcgctcaccg gagtcgaggt
     3361 gatctcgaac ggggtctccg cgttcaagaa gcctgaaccg aagaacgccg catttaccat
     3421 gatcggtatg gcggcgattc tcgggaccat gttcatcggc atcagcgcca tggcctacta
     3481 cttcggaatc gtgcccaaag aggatgagac cgtggtctcg caaatcgcac gcgccacctt
     3541 cggaacaggc ccgctctact tccttgtaca ggcatcgacc atggtcattt tgattctggc
     3601 cgccaatagc agcttcaacg gattcccgcg cctagcctcg atcctggccc gcgacagcta
     3661 catgccgcac caaatgtcca tgatgggaga ccggctcgtc ttctcgaacg gcgtcatcat
     3721 tctcggcgtc ttctcctgcc tgctgatcgt cctgttcaac ggcgacactc atgcgctgat
     3781 tcccctgtat gccgtgggcg tcttcctgtc cttcaccatc tcccaggccg ggatggtgaa
     3841 acgctggctc gtcaagaaag ggccgcattg ggaaaagaaa ctgctggtca acggcatcgg
     3901 cgccgtcaca accgccatcg ccacattgat tatcgccagc accaagttcg cccatggcgc
     3961 gtggatcgtc atcgtactta tcccgctgct cattacgttt tttcgcgcga tccgctccca
     4021 ctacaaggcc gtctcggaac aagtcgccct gtcccgggga caccgcccac ccatgccccg
     4081 gcgcaacatc gtcgtgctcc cgatcggcgg ggtcaatcgg gccgtcattc gcgccgtaga
     4141 ctatgcgcgg agccgctccg gagacattcg tgcggttctc gtcgatgtgg atccggaaga
     4201 gaccgcccgg gtggaaattc aatgggccca gtggggttgc ggcgtaccgc tcacggtgct
     4261 gccctctcct taccggtcgg tattgagttc cttactcgac tatctcgaac aggtgttgca
     4321 gaaagatcag gaatgttggg tcacggtggt gatcccggaa attctccccg cccgttggtg
     4381 gcaaaatatt ctgcacaatc aacgggcctt catgctgaag ggcgccctcc tgtttaagga
     4441 tcgcgtcatc ctaaccgacg tgccctacca cctcacgagg tgagcatgcg acagacacca
     4501 tcccgaggct cagtgaacag gcggaatacc ggagcgtgcc tacttgcgct caccttttta
     4561 ctatgcgtgc ccgtggggcc ggcattggcc tttaagattg tcgctccggc ggacggtgca
     4621 gtcttgacct ccggccaggc agttcctgtc gaggttgaag cagggaggga ggcaggcctc
     4681 gtcgaagtgc gctattactg gtatccggag cagaccgagg ccctggtaga acagggagaa
     4741 gaccgggcag ggaaggcttc ccagggaagc atggccacag agaagtattg gcagaaagac
     4801 agcatcaccg gggcgcctgt cgtggccctg cccgcgttga cctccaccgt cgaccgcgta
     4861 cccccctatg gtggcgcctt gcctgtccca tcggacgcca tcggccgcat gcgactcctg
     4921 gccatcgccg atatttcaca aggccggctt ggccgcaaga ccgtgtttga cgaagtcttt
     4981 gtcaccgtcc agcctgcagt tgacctgctg tccatcgact ttgagaccga gaaaccgctg
     5041 cagcttggtc gtaccggaca atcctccgcc tacgggcacg tcgactccct cggcaagatc
     5101 ttcgaactcc ccgtcgtggg cgaattcgcc gacggggtga tgcgccccct ctcgtcgccc
     5161 agtaccggta cgaagtacag ctccggcgac gaccatatcc tcaaagtgct acctgacggc
     5221 ctgctgcaga ttgtcggcaa cggcaagacc tcgctcaccg tcaccaatcg cggcaaacaa
     5281 gcgacgctcg acgtacgcgt ggaggtcaac cccgaggcaa acgaaccccc ggttgccgat
     5341 gccggcgccg ccaaaacggt caaggccggc gcgaaggtgc gcttgaacgg attgcagagt
     5401 cgcgaccctg agggcgaagc cctattttat gcttggagtc aggtgcgcgg cagcaaagtc
     5461 gcgatgctcg atgtcaacat ggcagaaccg tcgtttaccg cgccctatgt ctccgaaacc
     5521 cgcacctttc gcttcaagct gcgtgtgacg gataaaaagg gcgcggatag tgtgccggcg
     5581 tatgtggacg tcacggtcga gccttgacgg ctgctgaaaa aggcccccgg cggcgttctc
     5641 aggcgtgcgc ttccttgcga cgtaccataa aggtacgcct cagtcaccgc acgccttgcg
     5701 cccttgccgg atggcctttt tgagccgcct gcttttggcc acaagactct ggacactgct
     5761 ctcgacaatt aacctgatgg ctcccatcta tttcgcagaa gactgaaact atgctcccaa
     5821 cctccttcat cgatcgtctt agtcaaatga tgggcgtaaa gatggaagaa atcgctcagg
     5881 aaacacagga gggattttcc agagacagtg cggaagccgc caggagggat tgattcattc
     5941 aagtaatact cttggccttt atcaacgaag acgagttcaa cagatagata agcgcgtgaa
     6001 agctgtcctc gaatgccaaa agcgtctcgt ctccaccatg cgtcttccat tcagcgagac
     6061 cttggcttct gagcttaaag cacagtcaga ggaatgggta actccagagt ggtgcgagca
     6121 atctttgcag tccgaccccg acttgggctt gatgaaagac tacaaaactg gctttcgaga
     6181 agaaacccta caagccagga acagcgccct gagaaaagcc tccattgaaa ttgatctact
     6241 tgtcgatgaa ttgcgcacac agcgacccgc tgaaacactt actcttagta gggaactgga
     6301 ccagaaattc aaaattctat tgagccctgg tcggctgaaa aggatttcac agactggtct
     6361 caacaattga gctccgtgaa tggacaaatc gctgtgcttt ttgcagacct cgacaatttc
     6421 aaggctctca acaccaaata ctcagaaccc agaattgatc aaagcctgct ccctgatgcg
     6481 atgcatctcg tcgaaggcct tattcgcatg cgagggggcg cgtacaaata cggcggtgac
     6541 gagtacgtgc taatcctccc gaaccatgac gcgatagaag gggcaggctt cgcagaaaag
     6601 gttcgcaaag catttgaaca gttcgagttc agagtcgata cagacctggt acatatcacc
     6661 gtgtcccttg gaatcgcagt atggcctgtt cacagcacga cttatcaaga tctcctggtc
     6721 aaggcaagtg aggccaagac tcaagccaaa gttcagaaaa attgtttcag aatggctgaa
     6781 attattggcg gtccgggagc gccattaccg agaagtggtt tgaccctgtc cgctcaacac
     6841 ctggctgttt acttgaacag aaaatctcag catggactgg accacgaccc aatgatcgat
     6901 ccagcatcgc tgtgcaatga gctgagcttt acagaagatg tgttggctct agctgcggac
     6961 gaactcgcgg aacatggatg gatcaagttg atcaaaacaa tggggatggg gaaggcagga
     7021 ttctcgactc tctcgccaac cgccctgcta ttcatcgaga cagatcagtt catcagaggc
     7081 cgcgatccga aggctgatgc tagagaacta gccgcccaac ttatcaaaca acccgaagga
     7141 tcagcaaacc tatccgaact cgataaacag ttgtcatggg ggcctcggag gataaaccct
     7201 tccgtgtcat ttcttcagct gaaagaatat gtgcaagcct ctaaaacact gggcgcccat
     7261 ccctatgccg tttcatggat tcgagcaaca gagaagacta aacgtttcgc attaaacgtc
     7321 tagggttttg acaatcgcgc tgtccaccat tcatgcggac ggtgcggaat tcctcgaccg
     7381 ggcgagggga ttactttcgc ttgccgatct gagcccgtag ccactgcgtc aaggcgtcct
     7441 tactcagctc ccctgcggca acctgtcgcg tcgtgatttc cagcgcttca ggagcgggca
     7501 atttctcaat gccgttgaca tgcaggaaaa ccagtgcggc catgacagct acccgcttat
     7561 ttccgtctat gaaaggatgg ttttgagcga tgtgaaagtg ataggcggcg gccatggcag
     7621 tgaggtcccc atgtagaaac ttgccaccaa actgctgacg aggcatcgca actgctgcgt
     7681 ccaacaaccc atgggctctc actcctggcg agcccccgtc tattccaatc gtatcggcat
     7741 ggagaaagag cacatcctcc acaccgagaa agataaactc tctcacggac taatgcgcca
     7801 gccgcttcag catcggcccg tatcgcttgc gcaattcttt cgtaatggct gtcatccgct
     7861 caggaccgac gcccacgttc gcaggggtga caaccaacgc attcccgttt accgtcacct
     7921 gaagcggtgt ctcctccgtg atcccgagcg cttccatcat cggcttttcg atgacaaggg
     7981 ccatactatt tccgtgcctt ttcaatgtct taatcatggc gcacctcacg tatgaacaat
     8041 gtcagtacat gccagacctg ttgtccagca ggagccctcg cgaaacttgg gagacatttc
     8101 tgaagtgcac cgagaggcag acacctggca gaatccgaac tgaggcactc gccaccgatt
     8161 aaatcaacga cccttcgttg cgaagacggc gggcgctgcg agaaaggctc gctctcctgg
     8221 caaggtctac aggagacatg cggccaggac attcagtcca agactccgcc taccgtatct
     8281 tcttggatac tcatcgtatc ttgctggata cgacgactgg aagtatgacg ggacaatctg
     8341 ctcgaatcac gtccatcacg cggcggtgga aatccgcatc gtgctaccgg acccagagaa
     8401 ctgattttcc acaagaaatg ccgtggcgtt agcagtcact gccgggcagg caacatgaca
     8461 gtgcttagac cagactaaac agctctcgcg cacgggccaa ctcggctcga tggcggcgca
     8521 aggcgggacc gaacgacgac cggctcacat ctttttcatg ggtcagcatc tgttgttcga
     8581 tgtgcgccag cgcatcggcc aattgcttca ccacttgttt gcctccgcga gtcagccatt
     8641 tgaaatggcc gcccgcaaga tcgagatcct tcaacgagta ccgcaccgaa tcgatttctt
     8701 cgagacagcg atagcgggcc cgctcaagat cgcgcgggct gaggccagcc cttttccatc
     8761 gtgcagtccc cgcccgaccg gatgcccacg tagacagcct ctcgaacagc atcgccccca
     8821 tggtgaacgt gaaggtcgcg tgaagaatcc ctctcagcgg ccgcagactg cgccgccagg
     8881 gtgaataaaa aatctcctgg ttgtgatcgt gttgatacat cgcgtctttg cgcagcagca
     8941 aattaaggtg atggtggctg ttctcatgga tcagatcgtc gatcaaatcg agccggtcac
     9001 ggtcaaaaca attgatggca gacagcccgg gacgatgccg gtagctgaag ctcaccacac
     9061 cggaggcttt gagcggtacg acacgcgagg tcaataacgc cagcagccga tccccctccg
     9121 gccaggccga cgcaataaca gacagtgccc gtcgcatgcg tgcggcaatc tccggcctcg
     9181 tcggccgcac cgccaccggc gtcttgtcct ttccatagac cagtgtcggc ccgagtacaa
     9241 gcgcgccata ttggctctcc ctgaggcaca ggggctcgag acgccgccaa tgccaccgcg
     9301 ccttcttgcc gtatgcaacc agggcatagt cttgatcgcc gaccagcaga tcgatcccgg
     9361 acggcttgat cagaaattcc ccctgccctc ctgcccgttt gagcgccgat cgaaccgggg
     9421 ctggagccac ataggacaag cccgccgtgc cgaccgcaca gacccagggc aattcgacac
     9481 gttccacatt cgcattcaaa tcacagggca ccagccacgc gtccttaccc tgcccggcta
     9541 cccacgcaca agccagctgg ggagcaaggc agacagactc ttgcgtgatt tcccgcgcca
     9601 atcgctggca aaaggcggtc agtcgggaaa gaagcaggcg aggctcaggc ttgccgttag
     9661 ggaaaatctc atcggcatac ccgtgctcgt agaacttttc cctaaactcc gcgcggagct
     9721 gcgcggcaat ctctccgcga gatcgctcct gccgcacctg caccaggata tcgacgaaat
     9781 acagcaagtc gttcagcgac tcgatccacc cgaccacctt cccattgctg taatcctccg
     9841 aggtgaacga ctgtcccaac gtacgaaacc aatcgatcgg caacccgaac gtgcaggcag
     9901 cgtcggcata attccgttca aaatcctggc atacttcctt gagcaaccct cgcatcgagc
     9961 gccggaattc cgatatcagc tgttcgagta cccgcgcatc taataaggtc atcagctgca
    10021 gcctttctgg atcacatagg gtgtcgcgcg ggcactctac gcgagtgccc gccgactgac
    10081 aagccagcgg ggtggcaacc gactgcggaa atgaagtcac ctgcgataaa gcgatgggag
    10141 cagcgggaca gaacggtcga gagagcggac aagcctggcg gaggacgatt cgtcgaggct
    10201 gctgcgtcgg cggaacaacc ccgcgaaacc ggcgccggct tcgcggggga aggcctacgc
    10261 cgtcagcttg atcagatcaa agaggaacgg cttctcggga gccgagaccg gaacaggatg
    10321 gagatgagaa ttctgcacca tccgatgacg ctggatcagc gtatcgacca cattgccgca
    10381 gctcaagcac cggtgcccgc tcaaccgcat agggtgataa ctctcctgca gatcgatcaa
    10441 atcgtccgtc accatcaaac cttcacagcg cgtgcacgtc atgagtcgcc tcccttcgga
    10501 cagctgtgag atctcccgtc ctgtccggac agatctcgtg cccatgtgaa caactcaaca
    10561 accgtcaggc ctgaaaagaa gcaatgtgga tgccactgat cgcaccgggc catttcttca
    10621 cctggccgca agatgcgatc acaccaaaac cgatcctctc gcgacaaatt gactcggctg
    10681 tgaccgaatt ggtctcatgg gacaaaaatg ggtagaatcg actaactccg ctgaaaaacc
    10741 tatctaccct tgctgaatca gggtttagat ctatccctgc ggaccgggag tcacgtaccg
    10801 aaa
//
LOCUS       DAORRQ010000097        10611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_288_length_10611_cov_371.663698, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000097 DAORRQ010000000
VERSION     DAORRQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_10611_cov_371.663698"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PK224_18910"
     CDS             <1..303
                     /locus_tag="PK224_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17876.1"
                     /translation="LTPPDSPPMTTMYYSDGDQLMLTHYCSLNNQPRMRASAIKEGDK
                     TITFAFVDATNLKNPTDVHMRQLTIDVKDHDHFTQTWTLSKAGKDVPKVFTFERVK"
     gene            341..1198
                     /locus_tag="PK224_18915"
     CDS             341..1198
                     /locus_tag="PK224_18915"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB17877.1"
                     /translation="MPSQTPEAVIEAQRQDWNRVAAGWDKWDQFFNRNMAFINHRLVA
                     DARVRPGLRVLDLGSGTGYPALLAGEVVGSDGTVVGIDLAESMLAVATRKAKTLGLEH
                     VTFRTGDVTSLPFETGSIDAVISRFCLMFLPEIPKAAAEIARVLKPGGYVAAAVWSAP
                     DKNPFIRIPMDVIKTITPLPPPDPEAPGIFRLAKPGDLAGMFERAGLKPLDDEEFTAE
                     VTYATAEEFFRGLMDIAAPIQNLFAKLTQEQQAEAERGIVKAVNEYRGPQGVSLPIAV
                     RIVSARKPQ"
     gene            complement(1274..1552)
                     /locus_tag="PK224_18920"
     CDS             complement(1274..1552)
                     /locus_tag="PK224_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17878.1"
                     /translation="MRTFIIAAVAGFCLLTGLSLSAGAGETKAKIEKGKGELKGDLEE
                     MKGETKAMKEELKGNDTKAEMERAKGKLKGAGERAKGKMKELKEKATD"
     gene            1969..2136
                     /locus_tag="PK224_18925"
     CDS             1969..2136
                     /locus_tag="PK224_18925"
                     /inference="COORDINATES: protein motif:HMM:NF018715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HRB17879.1"
                     /translation="MLYYALMFLVVGLIANALNLLGVSAVAVQMSWILFLIGVVLLVV
                     HLVSGRTPRTT"
     gene            2231..2458
                     /locus_tag="PK224_18930"
     CDS             2231..2458
                     /locus_tag="PK224_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRB17880.1"
                     /translation="MNADQLKGKWLQFKGDLKQQWGKFTEDDLQQIAGNYDTFVGKVQ
                     ERYGEKKAELMKWASHWHEQAKPDAEGTKAP"
     gene            2521..3078
                     /locus_tag="PK224_18935"
     CDS             2521..3078
                     /locus_tag="PK224_18935"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRB17881.1"
                     /translation="MTTRNVLTLCAALVLGGAMITGSAHAIGKADEKTPINDSWITAK
                     TKIALFADSRVKGSEINVETAQGAVMIRGKVDSDAAKQAAEGIAKGIDGVKTVKNDLQ
                     VVAPSKREATDDKDDAITARVHEHMEKDAHLKKAGIHAQTNAGVVSLSGEVQDLATSA
                     EASWSAWQVPGVKAVKNDLTVKEKA"
     gene            3205..3426
                     /locus_tag="PK224_18940"
     CDS             3205..3426
                     /locus_tag="PK224_18940"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB17882.1"
                     /translation="METNVSRTTPQRAGTAHTSPSHDKAAHAGQAVKGTAAIALRAYE
                     LYEKRGRKDGQALEDWLNAERQLAAAAGH"
     gene            3483..3956
                     /locus_tag="PK224_18945"
     CDS             3483..3956
                     /locus_tag="PK224_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRB17883.1"
                     /translation="MSTLIRWDPFRVQRNPLKERDELEHRLSTLLGHRASTGNGGKEA
                     LTVAEWSPLVDIVEDENEYRIKAELPAMKKEDVRLTVDNGVLTISGERRYEQEEKREK
                     HHRIERAYGSFVRSFSLPEDADGSKVTADYKDGVLHMHLPKSEKAKPKSIEIKVS"
     gene            4272..4679
                     /locus_tag="PK224_18950"
     CDS             4272..4679
                     /locus_tag="PK224_18950"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRB17884.1"
                     /translation="MSSGPPNQSRRMRHDSFKDFVLDQLGEMPELTGKAMFGGYGLYQ
                     RATFFGIIHKGRLYFKTNDVTQALYRARGMHPFRPGAKQTLRQYYEVPVDILEDPQEL
                     AAWARSAVTLPTAQLPLPFTTAPPQGYDLPAQM"
     gene            4751..5122
                     /locus_tag="PK224_18955"
     CDS             4751..5122
                     /locus_tag="PK224_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HRB17885.1"
                     /translation="MWYRIGADLVLLLHLAFVLFVVTGALLLLKWPRLAWLHLPAAIW
                     GAIVEYTGWICPLTPIEHTLRAMTGESAYSSDFIGHYLLPLMYPAGLTRPVQMLLGTA
                     VVLVNVTIYWLVFGKPPRRRQ"
     gene            complement(5134..5718)
                     /locus_tag="PK224_18960"
     CDS             complement(5134..5718)
                     /locus_tag="PK224_18960"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB17886.1"
                     /translation="MTDPRHKEDMSEHAALYALEALGDDEQRQFKQALETAPPEVWQQ
                     VAAFQEVVQELAFSGPAIAPPASLKAKLMARIAQEPQERPGGGTFTFVRSAQGAWRDL
                     GLGVSMKMLFNDSAGARATMLLRLAPGGELVAHRHRQVEEFYVLEGSCICAGEFLQAG
                     DYHRAEAGSIHPITSSEEGCLALVMTSTQNEPAG"
     gene            complement(5715..6320)
                     /locus_tag="PK224_18965"
     CDS             complement(5715..6320)
                     /locus_tag="PK224_18965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRB17887.1"
                     /translation="MIHSQAVAQTPLMYEQEWAGLLARIAAGDQPALAEFYDASSAKV
                     FGLVMKILADRTVAEEVTMDVYTQVWRRASSYDAERGTPGSWLMTLAKTRAIDRFRSS
                     YLERGRQVPLDHAAEVPGDGATPEQYSAGLERQRLVQEAMASLSAEQRQAIALAYYWG
                     LSQSEIADRLQLPLGTVKTRMRLGMIRLREVLAPHGEGVRS"
     gene            6544..7020
                     /locus_tag="PK224_18970"
     CDS             6544..7020
                     /locus_tag="PK224_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HRB17888.1"
                     /translation="MSQRMASLSLILGLALTVAACNTVGSGPAGTTAGEKSLYDRLGG
                     KTAITAVVDDFVGRVAADTRINGKFANANIPRLKSMLVDQICQASGGPCTYTGRDMKS
                     THAGMGVSSGDFDALVGDLVATLNKFKVPEREKNELLGALGPMKGDIVEKPMASMQ"
     gene            7154..8341
                     /locus_tag="PK224_18975"
     CDS             7154..8341
                     /locus_tag="PK224_18975"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB17889.1"
                     /translation="MAIDDITQKVSDRYARAAATGEQMCCPTGYDFADLKSFVPEEVL
                     TISYGCGTPAGLNTVQSGETVLDIGSGGGIDCFEAARRVGPTGRVIGIDMTDTMLEIA
                     RRNAPIVAGNLGYATSNIEFRKGMADAMPVEDASVDLIISNCVINLAPDKRKVFREMY
                     RVIKPGGRFTISDIVSDQVVPQYLVHDAAKWGDCLSGALQVQDYIGGMVDAGFRAVHQ
                     IKSTPWQSIDGIHFLSVTLTGYKFPAIADEKTSSYATLRGPFSSVTDELGQRYERGVP
                     QSIDAQRAQLLQSEPLRSLFLLGPTPVTLAATDPRWCAIHPEQKPCVWQGAYAILTGP
                     IISAEDDDHHQYYRGVPLEICSKTLHVLTQEAYRPHFTIYQRASAGVDGTEVACSPTG
                     GCC"
     gene            8372..9409
                     /gene="arsS"
                     /locus_tag="PK224_18980"
     CDS             8372..9409
                     /gene="arsS"
                     /locus_tag="PK224_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HRB17890.1"
                     /translation="MGLSLLARRDPLAAASEQLRLLAQTTACQPFETALNAVELYPLH
                     ATGITTLQLNLGKLCNQTCRHCHVDAGPDRTEVMSKETLDLCLQALARTDIPTVDITG
                     GAPELNPHFRWVVEQARLLGRQVLDRCNLSVLLLPSQADLGEFLAQHRVEVIASLPSY
                     QATQTDAQRGEGIFDKSMEALRLLNRLGYGKEGSGLTLNLVYNPVGAFLPPKQEGIEA
                     KFRKELASRHGVSFTRLYTITNMPISRFLEFLIESGNYEGYMERLAAAFNPAAAAGVM
                     CRYTLSVGWDGTLYDCDFNQMLELPVSQGTPQHIRDFDPARLHHRQIVTRNHCYGCTA
                     GSGSSCGGAVT"
     gene            complement(9406..9846)
                     /locus_tag="PK224_18985"
     CDS             complement(9406..9846)
                     /locus_tag="PK224_18985"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRB17891.1"
                     /translation="MKARVLFLCTGNSARSQMAEGWLRHLAGDRFEVFSAGTHPVGLN
                     SGSVEAMAEVGIDIAGHRSKHVSEFLTQPFDYVITVCDRAKESCPTWPGPTHLLHWSF
                     DDPAAVTDSDEAHRQLFRRVRDEILGQIKGFLAQEAKSLVSGPC"
     gene            complement(9843..>10611)
                     /locus_tag="PK224_18990"
     CDS             complement(9843..>10611)
                     /locus_tag="PK224_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HRB17892.1"
                     /translation="AFFAWVFFRYVFSAWISPAEADQYIAGTIILAAAPCTAMVFVWS
                     YLTDGDPAYTLVQVAVNDLIMLVLFVPIVGLLVSGASSLAVPFDVLLYSVAIFIVIPL
                     IVGAGLRLWLVRRYGLRWMEEQFLPRFAPVTIAALLLTLVCIFAFQSQNILGKTVHVV
                     LIAVPILLQVYMNSALAYGLMHRWRVPYAIAAPGALIGASNFFELAVATAIALFGPES
                     GAALATVVGVLVEVPVMLSVCSLCNKTRHWFNQEVGT"
BASE COUNT         2238 a         3381 c         3011 g         1981 t
ORIGIN      
        1 ctcaccccgc ccgacagccc cccgatgacg accatgtact acagcgacgg cgaccaactg
       61 atgttgacgc actattgctc cctcaataac cagccgcgca tgcgcgcctc cgcgatcaaa
      121 gagggagata agacgatcac ctttgccttt gtggatgcca ccaacctcaa aaacccgacc
      181 gatgttcaca tgcgccagct gaccatcgac gtcaaagacc acgatcactt cacccagacc
      241 tggaccttga gcaaggcggg aaaagacgtg ccgaaggtgt ttaccttcga acgcgtgaaa
      301 taatcttcct ctctactttg tccaacaagg agtcgctccg atgccgtcac aaacaccaga
      361 ggctgtgatc gaagcccagc gtcaggactg gaatcgcgtt gccgccggtt gggacaaatg
      421 ggatcagttc ttcaaccgca acatggcctt catcaaccac cggctggtgg ccgatgcgcg
      481 ggtgcggcct gggctgcggg tgttggatct tggatcgggc accggctatc cggcgctgct
      541 ggccggtgaa gtggtgggat cggatggtac cgtggtcggg atcgatctcg ctgaatccat
      601 gctggcggtc gccacgcgca aagccaagac gctcggcctg gagcacgtga cgtttcgtac
      661 cggggatgtc acttcactgc cgttcgagac cggctcgatc gatgccgtga tcagccggtt
      721 ctgcttgatg ttcctgcctg aaattccgaa ggctgccgca gaaatcgccc gggtgttgaa
      781 accgggaggc tatgtcgcgg ccgccgtctg gtcggcgccg gacaagaatc cgttcattcg
      841 tatcccaatg gacgtgatca agaccatcac gcccctgccg ccgcccgacc ctgaggcgcc
      901 cggcatcttc cggcttgcaa agccgggcga cctggccggc atgttcgaac gggcgggcct
      961 caagccgctc gacgatgaag aattcacggc ggaggtgacc tatgccacag cggaggaatt
     1021 ctttcgcggc ctgatggata tcgccgcacc gattcaaaac ctgtttgcca aattgacgca
     1081 ggagcaacag gccgaagccg agcggggcat cgtcaaggcc gtgaacgagt atcgggggcc
     1141 acagggagtc tcgttgccga ttgcggtgag gatcgtgagc gcgcgcaagc cgcagtagcc
     1201 gtcgcacggc agcactcgca caataccgtg cccgccggcc ctgacgagcc ggcgggcggg
     1261 aaacgaacct gggctagtcc gttgcctttt ccttcaactc tttcatcttc cctttggccc
     1321 gctctcccgc gcctttcaat ttgcccttcg cccgttccat ctccgctttc gtgtcgttcc
     1381 ccttcaactc ttccttcatc gccttggttt cacccttcat ttcttcgaga tcgcccttca
     1441 gctccccctt gcccttttcg atcttggcct tggtttcccc cgcccctgcg gaaagcgaca
     1501 accccgttaa cagacagaac cccgcgacag ccgcaatgat gaatgtacgc atacccgtcc
     1561 tccacagatg gtgaattgct ctctttgtgt tgtacgtccg ctacggggac gacggcacta
     1621 gcgaaacccc tagccgtaca tcgagcatct ccccaattct tgcgactcgc gtttccactt
     1681 agcttcagcc tccgcgttcc ggcctcacat tatagtgctt gacgtcactg ccgattgcgc
     1741 ttagcgtgaa gaggtgccca atgaagctcc gctgactgtc ccatcctcgc gcttcccgtt
     1801 ccttcgtacg cgtgactgat tccccagtct ccgccgctcc tgtaccagag tctctctgag
     1861 gtgccgcctg tccgatgtgc cggtccgctc gtggagcgca accgtcattc ctcgaggccc
     1921 cggaggattg ttcaactcac gccggtgact ccatgaaagg agtgcccaat gctctactac
     1981 gcgctgatgt tcttagtagt cggcttgatt gccaacgccc tcaatctttt gggggtctcc
     2041 gcagttgccg tccagatgtc ttggatcttg ttcttgatcg gggtggtgtt gctggtggtg
     2101 cacctggtgt cgggacggac tccgcgaacc acctgacaac ggttcaggct gtctgaaccg
     2161 ttcaccgtat aaggacccga cagtcacacc cagccgaggt ttcatggctg acaccacgaa
     2221 aggaggcggt atgaacgcag atcaactcaa aggaaaatgg ctccagttca aaggggatct
     2281 gaaacaacaa tggggcaagt ttaccgaaga cgacttgcag cagatcgccg gcaattacga
     2341 cacctttgtc ggaaaagtgc aggaacggta cggcgagaag aaggccgagc tgatgaagtg
     2401 ggcttcccac tggcatgagc aggcgaagcc ggacgccgag ggaacgaagg ctccctgaat
     2461 cgacaccatc ggcgaaacag atttcgccgc acactttcac tgttcgctta aggagacttg
     2521 atgaccacac gcaatgtact gaccctctgc gcagcattgg ttcttggcgg ggcgatgatc
     2581 accggatcgg cgcatgccat cggcaaggcc gacgagaaga cacccatcaa tgactcctgg
     2641 attacggcga agaccaagat cgcgctgttt gccgattccc gggtgaaggg aagcgagatc
     2701 aacgtggaga ccgcccaggg cgcagtcatg atccgcggca aggtcgattc ggatgcggcc
     2761 aagcaggctg cagaagggat tgccaaaggg atcgacgggg tcaaaaccgt gaagaacgat
     2821 ctgcaggtag tggccccctc caaacgtgag gcgacggacg acaaggatga cgccatcacc
     2881 gcccgcgtcc acgagcacat ggagaaggac gcacacctga agaaagccgg cattcatgct
     2941 cagacgaacg caggcgtcgt ctcgctcagc ggcgaagttc aggacctggc gaccagcgcc
     3001 gaagcctctt ggtcggcctg gcaagtgccc ggcgtgaagg ctgtgaaaaa cgatctcacg
     3061 gtgaaggaaa aggcctaacc cttttcttca ttacacagac ccgctcttcg tccgccgaga
     3121 tccgacagaa gggtagcggc ggacggcaga gcctctgatg gtttcagccg ctacacagcc
     3181 ggctcaacaa ggaggtacac cataatggaa acgaatgtca gtcgaaccac cccgcagcgg
     3241 gccggcacag cccacacgag cccgagccat gacaaagccg cgcacgcggg gcaggccgta
     3301 aagggaaccg ctgcgatcgc cctacgcgcc tacgaactgt atgagaaacg agggcggaaa
     3361 gacgggcagg ctctggagga ctggttaaac gccgaacgtc agctggctgc cgcagcgggc
     3421 cactaatcgc accgacatcg agtcgtcatg atgccatttc gaccatacga ggagatgaag
     3481 ccatgagcac actcatacgt tgggatccgt ttcgagttca gcggaatcct ttgaaagaac
     3541 gggacgaact ggaacaccga ttgtcgaccc tcctgggaca tcgggcatcg accgggaatg
     3601 ggggcaagga agccctgacg gttgccgagt ggtcgccgtt ggtggacatc gtggaagatg
     3661 agaatgaata ccgcatcaag gcggagttgc cggccatgaa aaaagaagac gtgcgactca
     3721 ccgtggacaa cggggtgctg acgatttccg gcgaacggag atacgagcag gaagagaaaa
     3781 gggagaagca tcaccggatc gaacgcgcct acgggagttt cgtccgcagc ttctcactcc
     3841 cggaggatgc ggacgggagc aaggtcacag ccgactacaa agacggggtg ctccacatgc
     3901 atctcccgaa atccgagaaa gcaaaaccga aatcgatcga gatcaaggtg tcctagcctc
     3961 ctgccgctga gacccctgaa tcgcttgcgc ctcgtgggat gtgctcaaat catgcaatag
     4021 cgcatccacg gggcgaccgg gttcacgcgc gacgaggact gctgcagcac agagaccgca
     4081 catcctggtt tcagcctcca ccttccacac gttcatcaac tcgcacggct ccgtatacta
     4141 taaagcctcc ccctcacgct tgtgcctcga atgacgacca cccccctgga cacacaacgg
     4201 aatccctcgc cacccggcag ttgaaataat tgagtcatcg gtgcagagtg cgccgaatcg
     4261 atagggccga catgagcagc gggccaccca atcagtctcg acgcatgcgc cacgacagct
     4321 tcaaagactt cgtcctggat caactcggtg agatgcctga gctcacaggg aaagccatgt
     4381 tcggcgggta tggcctctac cagcgggcca ccttcttcgg catcatccac aagggacgcc
     4441 tctacttcaa aactaacgac gtgacgcaag cgctctatcg cgcgcgcggc atgcatccct
     4501 tccgtccggg cgccaaacaa acgctccgac agtattacga agtgccggtg gatattctgg
     4561 aggacccgca ggagctggcc gcctgggctc gcagcgccgt gacattaccc accgcgcagt
     4621 tgccccttcc cttcaccacc gccccgccgc agggctatga ccttcccgct cagatgtagg
     4681 gacgcggcaa ccggctccgc tcgccgtctc ctgattgcct tcatcgcgcc gtttcttcag
     4741 actgccgcac atgtggtacc gaatcggcgc ggatctcgtg ctgctgctac acctggcctt
     4801 tgtgctcttc gtcgtgacgg gcgcgctgct gctattgaaa tggcctcgcc tggcatggct
     4861 gcatctgccg gcggcgatct ggggagcgat tgtcgagtac accggctgga tctgtcccct
     4921 caccccgatt gaacacactc tgcgggccat gaccggagag tcggcctaca gctcagactt
     4981 catcggccat tacctcctgc ccctgatgta tccggcaggg ctgacacgcc ccgtgcagat
     5041 gctgctggga accgcggtgg tgctggtgaa tgtgacgatc tattggctgg tcttcggaaa
     5101 accgccaagg cgacggcaat agaagtgaat ggattacccg gctggttcgt tctgtgtcga
     5161 ggtcatcacc agggccaggc agccctcttc gcttgatgtg atgggatgga tgctcccggc
     5221 ctcggcgcga tggtagtccc ctgcctggag gaattcccca gcgcagatgc agctcccttc
     5281 cagcacgtag aactcttcga cctgcctgtg acgatgcgcc acaagttctc cgcccggagc
     5341 cagccgcaac aacatcgtag cccgcgcgcc agccgaatcg ttgaacagca ttttcatgga
     5401 tacaccgagg cccaaatccc gccacgcgcc ctgtgccgat cgaacaaacg tgaacgttcc
     5461 tccgccgggt cgttcctgcg gttcttgcgc gatgcgcgcc atgagcttgg ccttgagtga
     5521 cgccggaggc gcgatggcgg gaccgctgaa tgccaactct tgcaccacct cctgaaacgc
     5581 tgcgacctgc tgccagactt ccggaggagc cgtctcaagg gcctgcttga attgccgctg
     5641 ctcgtcatca cccagagctt ccaaggcgta gagtgccgca tgctccgaca tgtcttcctt
     5701 gtggcgtgga tcggtcatga ccgcacccct tccccgtgcg gagccaggac ctcgcgcagt
     5761 ctgatcatgc ccagcctcat ccgcgtcttc accgtcccca acggcagctg caaccggtcg
     5821 gcgatttcac tctgactcaa accccagtag tacgccaagg caatcgcctg acgctgctcg
     5881 gcggagagac tcgccatggc ttcctgcacc agccgctgcc gttcgagccc cgcactgtat
     5941 tgctcgggcg tggccccgtc acccggcacc tccgccgcgt gatcgagcgg gacttgccgc
     6001 ccccgctcca gataactgct ccggaaccgg tcgatggccc gggtcttcgc gagcgtcatc
     6061 agccagctcc ccggcgtgcc tctctccgcg tcgtagctgg aggcgcggcg ccacacctgc
     6121 gtatacacat ccatggtgac ttcttcagca accgtacgat ccgcgaggat cttcatcacc
     6181 aacccgaaca ccttggcact gctcgcatca tagaactccg caagcgcagg ctggtctccg
     6241 gcggcaatgc gcgcgagaag tcccgcccat tcctgctcat acatcaaagg tgtctgtgcc
     6301 acagcctgtg aatgaatcat gagcatcctc gtcaagtact acgagtctgc ccggtgaatt
     6361 agattccctc tgcctgcgcc gatccgccga aactgtagca tcggaacgat cggggcacaa
     6421 cgcagttttt ttcgttcccc ggaaatccaa ctgccatccc acctcgtagt tcgagacaga
     6481 gagccggtcg atgtcgagac acacccggcc caaccgtcag acccataacc aggaggtact
     6541 ctcatgtcgc aacgaatggc atccctttct ttgatccttg gcctcgcgct gaccgtcgcg
     6601 gcctgcaata cggtcggttc aggaccggcc ggtaccacag caggcgaaaa atctctctac
     6661 gaccggctgg gtggcaaaac ggccattacc gcagtggtgg acgacttcgt cgggcgcgtg
     6721 gccgccgaca cccgcatcaa cgggaagttc gccaacgcca atatcccgcg cctgaaatcg
     6781 atgctggtcg atcaaatctg tcaggcgtcg ggcggacctt gcacctatac cgggcgggat
     6841 atgaagagca cccatgccgg catgggggtc agcagcggcg acttcgacgc gctggtcgga
     6901 gacctcgtcg ccacgttgaa caaattcaaa gtgcctgaac gggaaaagaa cgagctgctg
     6961 ggcgcattgg gtccgatgaa gggcgacatt gtcgagaagc cgatggcctc gatgcagtag
     7021 ttcccgaacg tgtacgacac tcgtgacctg cgggggcagc cgaaccggct gcccccgcct
     7081 ctggttgttg acctgccgcc tcttctcttg atagatcatt cgacggcatt tcctatccca
     7141 ctccgaggac cttatggcca tcgacgatat cacgcagaaa gtcagtgacc gctatgcccg
     7201 ggccgccgca accggcgaac agatgtgctg cccgaccggg tatgactttg ccgacctcaa
     7261 atcgtttgtt cctgaagaag tcctaaccat ctcctacggc tgcggcactc cggccgggct
     7321 gaacaccgtt caatcgggtg aaacggtgct ggatatcggc tccggcggcg ggatcgattg
     7381 ttttgaagcg gcgcgccggg tgggacccac cggccgggtc atcggcatcg acatgacgga
     7441 caccatgctg gagattgccc gccgcaacgc gcccatcgtg gccggaaacc tgggctacgc
     7501 gacatcgaat atcgagttcc gcaaggggat ggccgatgcc atgccggtgg aggacgccag
     7561 cgtcgatctg attatttcca attgtgtgat caatctagcg cccgacaagc gaaaggtctt
     7621 ccgcgagatg taccgcgtga tcaagccggg cggccggttt acgatttccg acatcgtgtc
     7681 ggaccaggtt gtgccgcaat acctggtgca tgacgcagcc aagtggggcg actgtctgtc
     7741 cggcgccttg caggtgcagg actacatcgg cgggatggtc gatgcgggat ttcgcgccgt
     7801 ccaccagatc aagtccacgc cctggcagtc gatcgacggc atccatttcc tctcggtgac
     7861 gctcaccgga tacaagtttc cagccatcgc cgacgaaaag acctcgtcct atgccacgct
     7921 ccggggcccc ttctcgagcg tcacggacga attgggccaa cggtacgaac gcggcgtacc
     7981 ccaaagcatc gacgcccaac gcgcgcaact gttgcagagc gagccgcttc gttcgctgtt
     8041 ccttcttggc cctacgcccg tcacactcgc tgccaccgat ccacgctggt gcgcaatcca
     8101 tccggaacag aaaccctgtg tctggcaagg cgcctatgcg attctcaccg ggccgatcat
     8161 cagtgccgag gacgacgatc atcaccagta ctaccgcggc gtgccgttgg aaatttgttc
     8221 caagaccctc catgtcctga cacaggaggc ctatcgccct cacttcacga tctaccaacg
     8281 agcctcggca ggtgtggacg gcacggaagt cgcctgtagc ccgaccggcg gttgttgctg
     8341 aacccctgac gatgaggaag ggatcgatcc gatgggtcta agcctgctcg ctcgacgaga
     8401 tccgcttgca gccgcatcgg agcagctgcg actcctggcc cagacgacgg cctgtcagcc
     8461 gttcgagacg gccctcaatg cggtagagct ctatccgctt cacgcgaccg gcatcacgac
     8521 gctccaactt aacctcggca agctgtgtaa ccaaacctgt cgccactgtc acgtagacgc
     8581 cggaccggac cggacggagg tcatgtcgaa agagaccctc gacctctgtc tccaggccct
     8641 agctcgcacg gatattccca ccgtggatat caccggcggc gcacccgaac tgaatccgca
     8701 ctttcgctgg gtcgtcgaac aggcccgatt attgggccgt caggtgctgg atcgctgcaa
     8761 tctttccgtg ctgctcctgc cttcgcaagc cgacctggga gagtttctcg cacagcaccg
     8821 cgtggaggtc attgcatcgc tgccgtccta tcaggcgaca cagacggatg cgcaacgcgg
     8881 cgagggtatt ttcgacaaat cgatggaggc ccttcggtta ttgaaccgac tgggctacgg
     8941 caaagagggc agcggactga cgctcaacct ggtgtataac ccggtgggcg catttcttcc
     9001 gccgaagcag gaagggatcg aggcgaagtt ccggaaagag ctggcctccc gtcacggcgt
     9061 cagcttcacg cgcctgtaca ccatcaccaa tatgcccatc agccgatttc tggagttcct
     9121 gatcgaaagc ggcaactacg aaggctacat ggaacggctt gccgcggcct tcaacccggc
     9181 ggcggcagcg ggcgtcatgt gccgctacac cctgtcggtc ggatgggacg ggaccctcta
     9241 cgattgcgac ttcaatcaga tgctggaatt gccggtctcg cagggaaccc cacaacacat
     9301 tcgagacttc gatcccgcac gcctgcacca tcggcagatc gtgacgagga accactgcta
     9361 cgggtgcaca gccggctccg gttcctcctg cggcggcgcc gtcacctagc aaggacctga
     9421 gacaagtgac tttgcctcct gagccaggaa acccttgatc tgtccaagaa tctcgtcgcg
     9481 aactcgtcga aacagctgtc gatgggcttc gtcagagtcg gtaactgccg ccggatcgtc
     9541 aaaactccaa tgcaacagat gggtgggccc cggccaggtc gggcaggact ctttggcccg
     9601 gtcgcagacc gtaatgacgt aatcgaacgg ctgcgtcaga aattcagaga catgttttga
     9661 ccgatgtccc gcaatgtcga ttcccacctc ggccatcgct tcgaccgagc cagagttcaa
     9721 gccaaccggg tgagtgcccg cgctgaacac ctcaaaccga tcgcccgcca gatgccgcaa
     9781 ccacccctcc gccatctgac tgcgcgcgga gttgccggta cagagaaata gcactcgtgc
     9841 tttcatgtgc cgacctcctg attgaaccaa tgcctggtct tgttgcacag gctgcagacg
     9901 gacaacatga ccggcacttc caccaacacc ccgaccaccg tcgccaacgc ggcaccggac
     9961 tccggtccga acagggcgat ggcggtcgcc acagccaatt cgaaaaaatt gctggcaccg
    10021 atgagcgctc ccggcgcggc gatcgcgtag ggaacccgcc agcgatgcat caggccatag
    10081 gcgagcgccg aattcatgta gacctgcagg agaatcggca ctgcaatcag gacgacatgt
    10141 acggttttcc ccaggatgtt ttgcgactga aacgcgaaga tacacaccaa cgtgaggagc
    10201 agcgccgcga tcgtcaccgg cgcgaatcgg ggcagaaact gttcttccat ccaacgcagc
    10261 ccgtaccgac gaaccagcca cagtcgaagc ccggcgccga cgatcaacgg aatgacgata
    10321 aagatcgcca ccgaatagag cagcacgtcg aacggcacgg ccaatgatga cgcgccgctc
    10381 accagcaacc cgacaatcgg aacaaacagc acgagcatga tcaggtcgtt cacggccacc
    10441 tgaacgagcg tgtacgcggg gtctccgtcc gtcaggtaac tccacacaaa caccattgcc
    10501 gtgcagggcg ccgcagccag gataatggtg cccgcgatgt attggtcggc ctcggcgggt
    10561 gagatccagg ctgagaagac gtaccggaaa aacacccagg caaagaacgc c
//
LOCUS       DAORRQ010000098        10218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_295_length_10218_cov_105.333957, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000098 DAORRQ010000000
VERSION     DAORRQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_295_length_10218_cov_105.333957"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="tdh"
                     /locus_tag="PK224_18995"
     CDS             complement(<1..902)
                     /gene="tdh"
                     /locus_tag="PK224_18995"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRB17893.1"
                     /translation="MRALVKTDAQPGLTYTNRPDPTPGPSEAVIRVKATSLCGTDAHI
                     YNWDAWAHSRIHPPRTIGHEMCGEVVAVGTDVTLVRVGDYVAAESHLTCGACFQCRTG
                     QAHVCKNYRILGVDLDGSFADYVVLPETVLWKTSPDIPPELACLQEPLGNAVDAALVE
                     DLTGHTVLITGCGPTGLFAAAVARTAGAATIIATDVSDYRLSLAKQVGVDHVFNAKTD
                     GPEAIAAAILDITRGEGVDASLEMSGHPTALHHAFRSVKNGGRVTLFGIPTGPVTCDL
                     ANEVIFKGIRVHGITGRRLFSTWYR"
     gene            1065..2090
                     /locus_tag="PK224_19000"
     CDS             1065..2090
                     /locus_tag="PK224_19000"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRB17894.1"
                     /translation="MMRYTFGRVPGPALGGFWLVLGLLIMAAQAAAGASPASTPTPAK
                     RWAQFELVSTEPDGSVQVGKDVVLGITLGGVPAGTESVMAIIESPGFQSQTVSLSEDA
                     APAVFQGTAILEPNSILKSRTTVHPKAVRIRVSFARAKITGLEEFLKRDVYVTLGDRP
                     VEEAEAEVVPGPPAESPEADAEAVQKATEQVMAVNATIAEEDLLPLPPPGESKAYWKQ
                     VSDLISRNWSRQIRSIRRAPSSETVRVHFKMYASGVAQLIQLEKGSGARDVNDAGLQT
                     IIHAHPFPPIPPDISSDVVDVHVRMRTGAKVATRDVQMTVEKKPSKPVVSPSPKEGTA
                     ASGPAKE"
     gene            2093..2704
                     /locus_tag="PK224_19005"
     CDS             2093..2704
                     /locus_tag="PK224_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRB17895.1"
                     /translation="MTNRIGRAMAMWCMGATVLFGATLGHAETARYDVDLDHSIVEFK
                     VAHMVISKTTGHFKDYTGFIEMDPDAGTVKALEATIKSASVTTNHEKRDAHLRNPDFF
                     DVEKYPMITYKMKSHKKTGDGYTAVGDLTLHGVTKEVTLTGNFNGVTKDPWGNTRAGF
                     TAEGKVNRKDFGMVWNKALDSGGLVVGDEVFIKLDIECIKAKS"
     gene            3072..3668
                     /locus_tag="PK224_19010"
     CDS             3072..3668
                     /locus_tag="PK224_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17896.1"
                     /translation="MWVGRLVTLLAVLWSLAAAEAGALEFTADQITKINGRTQKSNIF
                     YRDNMWRIEHHTMGPVNISIVRKDKKVVWLLLSRMKHFKTVPYQAEQDLKVSERLEGE
                     VSREEIGTETREGHPTTLYEVTVKEGERTEVYYQWLATDIHFPMKLAKKDGSWIVEYQ
                     HVKFRPLIDYLFQLPLNFEPLEEFDHQPAAADSSQQPM"
     gene            3699..4625
                     /locus_tag="PK224_19015"
     CDS             3699..4625
                     /locus_tag="PK224_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17897.1"
                     /translation="MRKAGMTVWALGVAVLGMTGSLYALDVADVVREWTPEGKKLAME
                     RAKLPAHDEMIRIPAGEFLMGSDRKVDKNSYLAEFPQRKIYLDAYEIDKYEVTTVQFL
                     KFVLASNRDPLIDWQYDGGNFQETMVSHPVMHVSWFDAEAYCKWAGKRLPTEAEWEKA
                     ARGEDGRIYPWGNQMAGLSRANFGRTGLSGPVRDRPERLLLYPPIISVDKYDNAASPY
                     GVLQMAGNVAEWVADWYDPKYYTTGPDKNPKGPETGSQRSFRGGGWIDSTPSVRVAQR
                     NGTDPNTKMNWMGFRCARDVNEATSGQPTEAK"
     gene            4689..5156
                     /locus_tag="PK224_19020"
     CDS             4689..5156
                     /locus_tag="PK224_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17898.1"
                     /translation="MSLVLLLAACQIEDLNIEIVNEDGSVLFIVHENDAPDPAPLSRF
                     GVVSHYSGARWEVSTYEESSLYRMQDGKKLLKPLDELPEINALAAGAIRFGEVPRGFF
                     IQGSSGNHLVALEEGRVYEAYANRGAHRGHISFTIEHGVAVKLPPVTRPLSSP"
     gene            complement(5131..5973)
                     /locus_tag="PK224_19025"
     CDS             complement(5131..5973)
                     /locus_tag="PK224_19025"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRB17899.1"
                     /translation="MAGAGFNGLTVAAFESRMAAEMTRLIERHGGKPLVAPALQEIPL
                     EDNSAALQFGELLLTEGLDVLVLLTGAGTTTLFEILHSRHAKDTIKNALKETVLIARG
                     PKPVAALKTLGFQPTLTVPEPNTWVDVVSTLDAYRPVKGLRVAVQEYGLPNRELLEAL
                     KQRGANVVQVPVYRWALPEDTAPLKLVINQILAGQVQVMLVTNAAQIDHVMQVVEQEG
                     NTAQFKTTCKKLVIASIGPTASERIRNHGLTVDFEPSHGKMGILVKETSETAHSLMAR
                     KAGA"
     gene            complement(6015..6854)
                     /locus_tag="PK224_19030"
     CDS             complement(6015..6854)
                     /locus_tag="PK224_19030"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRB17900.1"
                     /translation="MVARSVLAIVVALIGLNGVSLHAFAAQQAALPKEITGKDGAPLV
                     LIPAGSYPMGVPTGDRDGGRDEYPRHVIEITDFYIDKYEVTNGRYLEFVKATNHRVPQ
                     NPKNPTRNLWEGIGIPASLADRPVINVDWADADAYCKWAGRRLPREAEWEKAAKGNND
                     WRFPWGNVEPTDKHLNFNQKWIGEKTLMPVGSYESGKSPYGAYDMAGNVWEWVNDWYD
                     AKYYEKSPDKNPPGPASGEKRVIRGAGWQNETPTVRIFTRVDSDPTIRNESTGFRCAM
                     DAR"
     gene            7028..7459
                     /locus_tag="PK224_19035"
     CDS             7028..7459
                     /locus_tag="PK224_19035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRB17901.1"
                     /translation="MPTHRGLRHLALRVTDLARSRAFYERFLAMKVVWEPDPDNVYFS
                     SGSDNLALHQIAPAELAQYQPLRGQLLDHVGVIMESPAHVDDLFRDVQQDGARYGATI
                     AKPPKQHRDGSYSFYFADPDGNVIQALYEPTISRMEFRPPA"
     gene            7471..9087
                     /locus_tag="PK224_19040"
     CDS             7471..9087
                     /locus_tag="PK224_19040"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB17902.1"
                     /translation="MWDTLARDQYVGLAPWVWVQLESNQPPGPFPFIGGVAPEVVASL
                     HEAHSLFLACVETAISDVFSRRATLGDPQTRVRLEDAYAELVNSRQQLSQHITARRQP
                     DGQFHWSHPFDATKSATVVNTGLRIFNAVKRQAIPVPFERPMGPVVGKLLGMLDGTQQ
                     AGAIQTIVATAGRDGERLLTKLMALLVQYECLTPTKHASIRDQWLANTKDRDTIHLGH
                     AALMYRQQDRFLLFDPWLLPWFAESNVPSLWVSMLPKPGAIFLTHDHDDHVDPRTLLH
                     VPKDVPIVIPSRRNRKKFFYDYLPLLRELGFTHIVELAHGETWNFDGGAVVSVPFYGE
                     DPCDLEMPRNCYLVTDRGQNVLVHADSGPTNSGRSAIKEGVLQQLVQKYGPISLVLAS
                     QQQLQEIRSYAAHAPLSHPGLWLEVGENGYLTNGYLDELCAVSRAKLFVSYATGGADW
                     YPDHLSFMFSQRNPARTALLTANWEQPEKLKDLLGMRGCGYHRAHALDLFRSSGDGAV
                     QVSSAAEALAPLSLYRLDHGDPAFMKPSGR"
     gene            9353..>10218
                     /locus_tag="PK224_19045"
     CDS             9353..>10218
                     /locus_tag="PK224_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HRB17903.1"
                     /translation="MGSGKGLVLVTGAAGFIGSHVSRRLLDRGDTVLGLDNLNDYYDV
                     RLKEARLARLSSHPQFQFVKLDLSDRAGIAALFEQHPIRRVVHLAAQAGVRYSLVNPH
                     AYTASNIDGFLNILEGCRHHKAEHLVYASTSSVYGGHTKMPFSVHDNVDHPVSLYAAT
                     KKANELMAHCYAHLYRFPITGLRFFTVYGPWGRPDMALFLFTKAILEGKPIDVFNHGK
                     MQRDFTYVDDIAEGVLRTLDRPAQADPAWASDNPDPGSSSAPYRLYNIGNHQPVELLR
                     FIEVLEQTLGMKA"
BASE COUNT         2039 a         2903 c         3214 g         2062 t
ORIGIN      
        1 cgataccagg tgctgaatag gcgacgaccg gtaataccat ggacacggat gcctttgaag
       61 atgacctcgt tggcgaggtc gcaggtgacg gggccggtgg gaatgccgaa cagcgtgacg
      121 cggccgccgt ttttcaccga gcggaaggcg tggtgcaagg ccgtggggtg tccggacatt
      181 tccaacgacg catcgacgcc ttctcccctc gtgatgtcga gaatcgctgc ggcaatggct
      241 tccgggccgt cggtcttggc gttgaatacg tgatcgacgc ccacttgttt ggccaggctc
      301 aggcggtaat cactgacgtc ggtggcgata atggtagcgg ctcctgcggt gcgggcgacg
      361 gccgccgcga acaggccggt cgggccgcag ccggtgatca gaacggtgtg gccggtcaag
      421 tcttccacga gggccgcatc gacggcattg cccaaaggtt cttgtaggca agccagctcc
      481 ggcggaatgt ccggagacgt cttccaaagc acggtttcag ggagcacgac gtagtcggca
      541 aacgagccat ccagatcgac gccgagaatc cggtagttct tgcacacgtg ggcctggccc
      601 gtgcggcatt gaaagcaggc gccgcaggtc aggtgcgatt cggctgcgac atagtcgccg
      661 acgcgcacga gcgtgacatc cgtcccgacg gccactacct cgccgcacat ttcatgtccg
      721 attgtcctgg gaggatgaat gcggctgtga gcccaggcat cccagttata gatgtgggca
      781 tcggttccac acagcgacgt ggctttgacc cggatgacgg cctcagatgg gccgggtgtg
      841 ggatctgggc gattagtata ggtcagcccc ggttgagcgt cggtcttcac gagtgcgcgc
      901 atgggctcat tataacaaga cggtgggaaa tgcctagccg aagcgagaac ttgcccggcc
      961 aggttagttt aggcggtttc ctgcctgaat gtgagcatgc cgttcgtccg aatggcgggg
     1021 gcaaaatacg ggttgaactg ggtagggctc ctgggtagga tgcgatgatg cgatacacgt
     1081 tcggcagggt tccgggcccg gctctcggcg gattttggct cgtactaggc cttctgatca
     1141 tggctgccca ggccgcagcc ggtgcctctc ctgcctcgac gccgacgccc gcgaagcggt
     1201 gggcgcaatt cgaactggtc agcacggagc cggatggatc ggtccaggtc ggcaaggacg
     1261 tcgtgctcgg catcaccctt ggcggggtgc ccgcaggaac cgagtccgtc atggcgatca
     1321 tcgagtctcc cggctttcag agtcagacgg tatcgttgag cgaagacgcc gctcccgcgg
     1381 tctttcaggg cacagcgatt ctggagccca attccatatt gaagagccgg accaccgtgc
     1441 atccgaaggc cgtgcggatt cgggtgtcgt tcgctcgcgc aaaaatcacg gggctcgaag
     1501 agtttctcaa gcgtgatgtc tatgtcacgc tcggcgaccg gccggtcgaa gaagccgaag
     1561 ccgaagtggt tcccgggccg cctgccgaga gccctgaagc cgatgccgag gccgtacaaa
     1621 aggcgacgga acaggtgatg gcggtgaacg cgaccatcgc cgaggaagat ctcttgcccc
     1681 taccgccccc aggagaatcc aaagcctatt ggaaacaagt gagcgatttg atcagccgga
     1741 actggagccg gcagatacgc tcgatacgcc gggctcccag cagcgagacg gtgcgtgtgc
     1801 atttcaagat gtatgcaagc ggtgtggcgc agttgattca gttggagaaa ggctcgggag
     1861 cgcgggatgt caacgacgcc gggttgcaaa cgatcatcca cgcccatccg ttccctccga
     1921 taccgccgga tatcagcagc gatgtcgtcg atgtgcatgt gcgcatgcga acgggggcga
     1981 aagtcgcgac ccgcgacgtg cagatgacgg tcgagaaaaa accgtccaag cctgtcgtgt
     2041 ccccatcacc gaaggaaggc accgccgcaa gcggtcctgc aaaggagtag ctatgacgaa
     2101 tcggatcggt cgtgcgatgg ccatgtggtg tatgggcgca acggtgctgt tcggcgcgac
     2161 gctcgggcat gccgagacgg cgcgatacga tgtggatctg gatcattcga tcgtggagtt
     2221 caaggtcgcc catatggtga tttcaaaaac caccgggcat ttcaaagact acacgggatt
     2281 tatcgaaatg gaccccgatg ccgggacggt caaggcgctc gaagcgacga tcaagtcggc
     2341 ctcggtgacg accaaccatg aaaagcgcga cgcgcatctg cgcaatcccg atttcttcga
     2401 tgtcgagaag tacccgatga tcacgtacaa aatgaagagc cacaaaaaga cgggcgacgg
     2461 ctataccgcc gtcggtgatc tgactctgca tggcgtgacc aaagaggtca cgctcaccgg
     2521 caatttcaac ggggtgacca aggatccgtg ggggaatacc cgggccggat ttacggccga
     2581 aggcaaagtg aaccggaaag acttcggcat ggtgtggaac aaagccctcg atagcggagg
     2641 gctggtagtt ggagacgagg tctttatcaa gctggacatc gagtgtatta aggccaagtc
     2701 gtaagtcgaa caccccctct ctcagaggcc gcacgcctgc ccggtcacca tgccgggcag
     2761 gccgatccag ccgcggcgca tgcagcgagg tctctctgaa tcagcgctgc gggccgccct
     2821 tccgtgatac cggttgttac cgacacagca gctggttgag gcgcagggag gatgtccacg
     2881 ggcatccggt tctcttgtcg ggctggtcct gtgcccgcct cactggcggc tcggggctgt
     2941 atgccgatgg gtggtgtggg tgatggatca gcaagggtac caagggtaat tcggaggccg
     3001 agagtaggag catcgtcatg tgtcgcaagc gggctggaaa aatcgtacct ggcatcggta
     3061 ggatacgcgg gatgtgggtg ggcaggctgg tgacattgct ggcggtgctc tggagcctgg
     3121 ccgccgcgga ggccggggcg ctggagttta cggccgatca gatcaccaag atcaatggtc
     3181 gaacccagaa gtccaacatc ttctatcgtg acaacatgtg gcggatcgaa catcacacga
     3241 tggggcccgt caacatcagc atcgtgcgca aagataagaa ggtggtctgg ctcctgttgt
     3301 cgcgtatgaa gcacttcaag accgtgccgt accaggcgga gcaggatctg aaagtgtcgg
     3361 agcggctgga gggggaggtg tcgcgcgaag agatcgggac ggaaacgcgc gaggggcatc
     3421 cgaccacgct ctatgaagtg acggtgaagg aaggcgagcg gacggaagtc tactatcaat
     3481 ggctcgcgac cgatatccac ttcccgatga aactggccaa gaaagacggg agctggattg
     3541 tcgagtacca gcacgtcaaa ttcaggcccc tgatcgatta tctgtttcag ttgccgctca
     3601 atttcgagcc attggaagag ttcgatcacc agccggctgc cgcagactcc agtcagcagc
     3661 cgatgtaagc gatgatcggg aaataaagga gcagcattgt gcgtaaggcc ggaatgactg
     3721 tatgggctct gggtgtcgcg gtattaggaa tgactggttc cctttatgcg ctggatgttg
     3781 ccgatgtcgt tcgtgaatgg acgccggaag gcaagaagct ggcgatggag cgcgccaagt
     3841 tgccggcgca tgacgagatg atccgtatcc cggcgggcga gttcctcatg gggagcgata
     3901 gaaaagtcga caagaattct tacctggccg aatttcctca gcggaagatc tatctcgatg
     3961 cctatgagat cgacaagtat gaagtcacca cggtgcagtt cttgaagttt gtcctggcgt
     4021 cgaaccgcga ccccctcatc gattggcagt atgacggcgg gaattttcag gagacgatgg
     4081 tcagtcaccc ggtcatgcac gtgtcctggt tcgacgcaga ggcgtactgc aagtgggcgg
     4141 ggaaacggtt gccgaccgag gcggaatggg aaaaggccgc gcgtggtgag gatgggcgca
     4201 tttatccctg gggcaatcag atggccggac tctccagggc gaattttggc cgcacgggcc
     4261 tgtcaggacc ggtgcgagac cgccccgagc ggctgctgct ctatccgccg atcatttctg
     4321 tcgacaagta tgacaatgcc gccagcccct atggcgtgct tcagatggcg ggcaatgtgg
     4381 cggagtgggt tgcagactgg tacgacccga aatactacac caccggaccg gataagaatc
     4441 caaaaggtcc ggagaccggc agccaacgga gctttcgcgg cggaggctgg atcgacagca
     4501 cccccagcgt gcgtgtcgcg cagcgcaatg gcacggatcc caataccaaa atgaattgga
     4561 tggggttccg ctgtgcaagg gatgtgaacg aagcgacctc aggacagccg accgaagcga
     4621 agtagtcgga ggtgcctcac ctggcaattc ccacacagcg aaggaccaat cagctcgtct
     4681 caggcctgat gagccttgtt ctgctgctgg cagcctgtca gattgaggat ctcaacatcg
     4741 agattgtgaa tgaggatgga tcggttctat tcatcgttca cgaaaacgat gcaccggatc
     4801 ccgcacctct aagccggttc ggagtcgtct ctcactactc cggcgcgaga tgggaggttt
     4861 cgacctacga agagtcatcg ctgtacagga tgcaagacgg caagaagctg ctcaaaccgc
     4921 tggatgagtt gcccgagatc aatgctctcg cagcgggagc gattcggttc ggcgaggtgc
     4981 cgcgcgggtt tttcatccag ggctcctcag gcaatcacct cgtcgcgttg gaggaaggca
     5041 gagtctacga agcctatgcc aaccgaggcg cacaccgagg gcacatatca ttcacgattg
     5101 agcatggagt agccgtcaag cttcctcccg tcacgcgccc gctttcctcg ccataagcga
     5161 gtgggccgtc tcgctggttt ctttcaccag aatccccatc tttccgtgtg agggctcgaa
     5221 gtccacggtc aggccgtgat tgcggatgcg ttcgctggcg gtgggaccga tggaggcgat
     5281 gaccagcttc ttgcatgtcg ttttgaactg cgccgtgttt ccttcctgct ccacgacctg
     5341 catcacgtga tcgatctggg cggcattggt caccagcatc acctgcacct gcccggccag
     5401 aatctggttg atgaccagtt tcagtggagc cgtgtcttcc gggagtgccc agcggtagac
     5461 gggcacctga accacattgg ctcctcgctg cttcaacgcc tccagcaatt cgcggttcgg
     5521 taacccgtat tcttgcaccg ccacgcgtaa ccctttgacc gggcggtagg catccagtgt
     5581 cgagacgaca tccacccagg tgttgggctc cgggaccgtc aacgtcggtt ggaatcccag
     5641 ggttttgagc gcggccaccg gtttggggcc gcgcgcaatg agcaccgtct cttttaaagc
     5701 attcttgatc gtatctttgg cgtgacggct gtgcaggatt tcgaagaggg tggtcgtgcc
     5761 ggccccggtc agcaggacca gcacgtcgag gccttcagtc agcagcagct cgccgaattg
     5821 cagcgcggca gagttgtctt ccaacggaat ttcctgcaag gccggcgcca ccagcggctt
     5881 gccgccgtgg cgctcgatca gccgcgtcat ttccgccgcc atgcgcgatt caaatgcggc
     5941 gacggtcaac ccgttaaacc ctgcaccggc catcgtactc cgtgattcaa ccgccggtta
     6001 ccatgccgac tgagttacct ggcgtccatc gcacatcgaa acccggttga ttcgtttcga
     6061 atggtcggat cgctgtccac acgggtaaag atacgaaccg tgggagtctc attctgccac
     6121 ccggctcctc gaatgacccg cttctcgccg ctggccggtc ccgggggatt cttgtcgggg
     6181 ctcttttcgt agtacttcgc atcgtaccag tcgttgaccc attcccagac attgccggcc
     6241 atgtcatagg caccgtaggg actctttccg ctctcgtagc tgcccaccgg cataagcgtt
     6301 ttttcgccga tccacttctg gttgaagttg aggtgtttgt cggtcggttc cacattgccc
     6361 caggggaagc gccagtcgtt gttgcccttg gcggccttct cccattcggc ctcccgcggc
     6421 aatcgcctgc cggcccactt gcagtaggca tcggcatcgg cccaatcgac gttgatcacc
     6481 ggacggtcgg ccagggatgc gggaatgccg atcccttccc agaggttacg ggtcgggttc
     6541 ttgggattct gcggaacacg gtggttcgtg gccttcacga attccaggta gcgtccgttc
     6601 gtcacttcat atttgtcgat atagaaatcg gtgatctcga tgacgtggcg ggggtattca
     6661 tcgcgtccgc cgtcgcggtc tccggtcggc acgcccatcg ggtacgagcc tgccggaatc
     6721 aggaccaacg gcgcgccatc cttccccgtg atctctttcg ggagagccgc ctgttgcgct
     6781 gcgaatgcgt gcaaactcac accgttcagt ccgatgagcg ccaccactat tgcgaggacg
     6841 gatcgtgcca ccatagctca acactccttt ggttcgaaaa agacgatggc agatcgtacc
     6901 acagcggcgg agaattcaga cacggcttgg gcagagaatg tgtgcactcg gggtgggccg
     6961 atgcaggaga atgaggattg acggggtgac aggcccccac ctactatacg gccaaggaga
     7021 ttccgacgtg ccgacccatc gaggcttgcg acatctggcc cttcgtgtga ccgatcttgc
     7081 ccggtcgcgc gccttctatg agcggttcct ggccatgaaa gtggtgtggg aacccgatcc
     7141 ggacaatgtg tatttcagtt cggggtccga caacctggcc ctgcatcaga tcgcgcctgc
     7201 cgagctggct cagtaccagc cgttgcgtgg acagttgctc gatcatgtcg gtgtgattat
     7261 ggaaagcccg gcgcatgtag atgacctgtt ccgggacgtc caacaggatg gggcacgtta
     7321 cggggccacc atcgccaagc cacccaagca acatcgcgac ggcagctact ccttctactt
     7381 tgccgacccg gatggaaacg tcattcaggc cttatatgaa ccgaccatca gccgcatgga
     7441 gttccggcct cccgcctgac gagcatgggt atgtgggata cattggcgcg cgatcagtat
     7501 gtcgggttgg ctccctgggt gtgggtgcag cttgagagta atcagccgcc ggggccgttt
     7561 ccgttcatcg gcggggttgc cccggaggtg gtggcgagtt tgcacgaagc tcacagtctc
     7621 tttcttgcct gtgtggaaac cgccatcagc gatgtgttct cccgccgcgc gacacttggc
     7681 gacccgcaga ctagggtgcg gctggaagac gcctatgccg agctggtcaa ttcccgacaa
     7741 cagctgagtc agcacatcac ggcccggcgt cagccggatg ggcagtttca ctggtcgcat
     7801 cccttcgatg cgacgaaatc cgcgaccgtg gtcaacaccg gcctgcgaat attcaatgcc
     7861 gtgaaacgtc aggcgattcc tgtgccgttc gagcgtccca tgggaccggt ggtaggaaaa
     7921 ctgctgggga tgttggatgg cacgcagcag gccggggcga ttcagacgat cgtggcgacg
     7981 gccggtcggg acggcgagcg gctgttgacc aagttgatgg cgctgttggt ccaatacgaa
     8041 tgcctcacgc cgacgaagca cgcctcgatt cgagaccagt ggttggccaa taccaaggat
     8101 cgggatacga ttcatctggg ccatgccgcg ttgatgtacc ggcagcagga ccggttcctg
     8161 ttgtttgatc cttggctgtt gccctggttc gcggagtcga atgtgccgag cctctgggtc
     8221 tcgatgttgc cgaaaccggg cgcgatcttt ttgacccatg accacgatga ccacgtggat
     8281 ccccggaccc tgctgcatgt gccgaaagac gtgccgatcg tgattcccag ccggcgcaat
     8341 aggaagaagt ttttctacga ctacctgccg ctgcttcggg agctgggatt tactcacatc
     8401 gtcgaactgg cgcacgggga gacttggaac ttcgacggcg gcgccgtggt ctccgtcccg
     8461 ttttacggcg aagatccttg cgatttggag atgccgagaa actgctacct cgtgacggat
     8521 cgcgggcaga atgtgctggt gcatgcggac agcggtccga ccaacagcgg ccggtcggcc
     8581 atcaaggaag gggtgcttca gcagctcgtc cagaaatatg gtcccatctc gttggtattg
     8641 gcctcgcaac agcagctaca ggaaatccgt agttatgccg cccatgcgcc cctttcccac
     8701 ccgggccttt ggctcgaagt cggagagaac gggtatctca ccaacgggta tctggacgag
     8761 ctctgcgccg tctcccgggc gaagctgttc gtgtcctatg ccaccggcgg cgccgactgg
     8821 tatcccgatc acctgtcgtt tatgttcagc cagcggaatc cggcgcgcac cgcgcttctc
     8881 acggcgaact gggaacagcc ggaaaagcta aaagaccttc tcgggatgcg cgggtgcggg
     8941 tatcatcgcg ctcatgcgtt ggatctgttc cggagcagcg gcgacggggc ggtgcaggtg
     9001 tcatctgcgg ccgaggccct agcgcccctg tcgctctatc gactcgatca cggggatccg
     9061 gcgttcatga agcctagcgg acggtaatca gttagcaggc tgttcagaaa ggtcgtccag
     9121 cattgccacc gcaagcgaag aggcgaatcg tgctcattct tacccgccca ccccgagcat
     9181 gccgagacag gctctatgcc cgtgggcgta cggtgagctg ctgaggttca cgacgcgctg
     9241 aatacagcgc gtcacgcttg tgaacgccgc cgagacggtg aggcggcagt gtcttgcgag
     9301 accgacactg gagagctttt tcaacagcct gttaggaaag gaagggagcg atatggggtc
     9361 tgggaaagga ttagtgctcg tcaccggcgc cgccggattt atcgggtctc atgtgagccg
     9421 gcgattgctc gatcgcggtg acaccgtgct cggtctggac aatctgaatg actactatga
     9481 cgtgcgtctc aaggaagcgc ggctggcccg cctgagttct catccccaat ttcaattcgt
     9541 caaactggat ctgtccgatc gtgcgggtat agcggcgctc ttcgaacagc atcccattcg
     9601 gcgcgtggtg catctggcgg cgcaggccgg cgtccggtat tccctggtca atccgcacgc
     9661 ctacaccgcc agtaacatcg atgggttcct gaacattctc gaaggctgtc ggcatcacaa
     9721 ggctgagcac ttggtctatg cctccacgag ttcggtatac ggcggccaca cgaagatgcc
     9781 gttctcggtg catgacaacg tcgatcatcc tgtgtcgctc tacgccgcca ccaagaaggc
     9841 gaacgagctc atggcgcatt gttatgcgca cctgtatcgg ttcccgatta cgggattacg
     9901 gttctttact gtctacgggc cctggggccg tccggatatg gcattgtttc tctttacgaa
     9961 ggcgattctg gaaggaaagc ccatcgacgt gttcaaccac gggaagatgc agcgtgactt
    10021 tacctatgtc gacgatatcg ccgaaggagt gttgcgcacg cttgatcggc ccgctcaagc
    10081 cgacccggcc tgggcgagtg acaaccccga ccccggcagc agctccgcac cctatcggct
    10141 gtacaacatc ggcaatcacc agccggtcga gctgttgcga tttatcgagg tgctggaaca
    10201 gacgctgggc atgaaggc
//
LOCUS       DAORRQ010000099        10105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_298_length_10105_cov_254.668358, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000099 DAORRQ010000000
VERSION     DAORRQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_298_length_10105_cov_254.668358"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK224_19050"
     CDS             <1..183
                     /locus_tag="PK224_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17904.1"
                     /translation="APTKQSLLDPEFQMCAGIFNTSSEVFSRLLDPESRDQAEYRANQ
                     YQDERCLFFFVRADSS"
     gene            742..1191
                     /locus_tag="PK224_19055"
     CDS             742..1191
                     /locus_tag="PK224_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17905.1"
                     /translation="MKKIPALSLAACVAWQMAWSPSWATPAYPEPSEQEMRQAIEQAM
                     VQRGGSQTRPGEISVDNPINGMTMTLTAFTKLGCEPANRGPGYVCSYHVKTKMSAHSN
                     EGSRQGDNHAAAVNQLLRAMMGGQEEVADTATRRFLRMGDQWVMSRE"
     gene            1571..4192
                     /locus_tag="PK224_19060"
     CDS             1571..4192
                     /locus_tag="PK224_19060"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB17906.1"
                     /translation="MNSEVQDMYRSLFIKQQYQRVGSNMLYGFLVVGILLCGTFLPFI
                     AVQNVTAEVNLTDGKDGITSSGTNLEGQISIVYQGKNSLGTVHEQQVAELAERLRARV
                     TPPPGPFSTILFEKLVKVLTYSDPAHAEDRELIKNVQEFLDGKIRDSLFVAYLEEVLP
                     KEVLPPVQATQALTLLQEKVKNGDLWAMFYLARANFHAANNSRVIQLQEQFEGLALPE
                     ATREYWDAYQQLLQDKNFPGSLEGCVKLAKEGQSKRFAPLINILGVQTMLGLGVQKDE
                     ALAFKLFREAANAGFAMSMTAVASQYLQGNGVQKDQAEAVRWYRKAAESGYASAMASL
                     ADMYAQGAGVGKDESEAAKWFHKAAHLGISDAMVSLATAYELGRGLMKDQGEAVNWYQ
                     RAADRGNAQGMADLGRIYTYGIGIDKDLKQGTNWLQKAAELGNSDGMTQLGYVYAEGV
                     GVEKDPSRAMHWYRKAAGLGHTQAMVLLGGMYEQGRAVPVDEKHAVALYEQAAKAGNE
                     NGRQAFERLKSDIEKRGTQAVREWADRYQRAPNISGGEFLKLVSSAKVDFRFTTNFEK
                     YVDAENMTRRVRQAIEKEGIRIVEDAPITIRVVWYLNLQTIQTTGGGGREAHPNYLYA
                     ETIDFEVVSPLYRDKSFHNLRVVPLTAFYAGTALSGEWVVDKTIRETVVVDLLKGIAE
                     EFSALRSPDDRGKDTARTNQVSWSAEANESMYRQYQMAKHQAGDDVERVFKGLTSVSS
                     ITTRLTGKAGKFLDETDVNRVWKVGLQEARLEQASDGALWVAHRIHLDKENGSKFSLL
                     FGNLSSIWMGPMLSWITVGERGTVFEFAGKLLRMDVVLWTDIRYTSEFWEEVGSKLPE
                     FVHGRIRDCVKETRFRR"
     gene            complement(4254..5383)
                     /locus_tag="PK224_19065"
     CDS             complement(join(4254..5134,5134..5383))
                     /locus_tag="PK224_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611480.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB17907.1"
                     /translation="MTRKRHTEEQIIAVLKDAQAGIGVPELCRKHGISDATFYKWRTK
                     YAGLEVSDVKKLRQLEDENRRLKQMVAEQALDIQALKAINRKKLVGPKAKRTAAQWMA
                     ERFGLSQRRVCRLLELDRNTLRYRSRRQEDAALRTRIREIAEAKRRYGCPRIYVRLRR
                     EGWRVNHKKVERLYYRDEGLSLRRRRRKKAAAVPRVALPTPTQPGRCYAMDFVHDRLA
                     NGRRFKCLTMTDLCSKEVPVIEVDVSIGGARVCRILNQLFTTRPLPETVILDNGPEFA
                     GMALDAWAAQHGVHLHFIQPGKPVQNAFIESFNGKFRDECLNEHWFLTLQEAQLVIEA
                     WRREYNEERTHSTIGDVTPQEFINTYQGGAHVAQQSTSLAVV"
     gene            complement(5481..6341)
                     /locus_tag="PK224_19070"
     CDS             complement(5481..6341)
                     /locus_tag="PK224_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17908.1"
                     /translation="MVHFVLILVQLFVAIEYAAAGGSKFACSPDPKEPNTGIFSFTGK
                     IEYEDGPPFQRELKSCLDKQGKIGRIDLDSTGGSVKAAFIMAEEISKYGFRTHVPAGS
                     RCVSACTLVFLGGRRRTKDLDGTYEVHAYSAYKYDGNSGFTYTGIPDNASVLLTLELL
                     HTLSAQMKSGQTASLLTRIPVPETTQEGWAWPKGERDKYAQVLNSPEIQKLVSALADQ
                     LSTNITKIERNTQKTVLEYYRFVQKQRVSSKFLDHMFDPTIKSLQVMSDKELSDFAVI
                     TEPARSYASE"
     gene            complement(6369..7121)
                     /locus_tag="PK224_19075"
     CDS             complement(6369..7121)
                     /locus_tag="PK224_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17909.1"
                     /translation="MALQFSRSNRNRNGSMRTIAPVASLCLLLSSCQGMYGQPNQAGQ
                     SNSSYVGGCVAGAALGAGGSLLWDILKAGSQKGSRVPTPTSESLKKAALIGGAGCAVG
                     LAATAIGKYLTEREQQRQDEVFQKAAQAGAEQAERERLQVEERYRNMPAPATDADRIS
                     REQARQREIEAAQSKQSSPESWSEGSTKGQAIYIGPTKSPQTGEVMRTAGGKGCVAIK
                     EIVMKDGVEASQETNACPASDGHWQRIELQRA"
     gene            complement(7141..7605)
                     /locus_tag="PK224_19080"
     CDS             complement(7141..7605)
                     /locus_tag="PK224_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17910.1"
                     /translation="MAGKLYAATKRPDAWNQRVQAADVQRALNIHMTPDSSEIAGWEL
                     TLAQGERFLQHDEFLSLFDTRTAECEKAAIQRIIEHTKQWETKVAEAGATPQRLLKQC
                     QAIGENHKQLSLDLRNAESKLAHAEEELNRLRESSSNGMESKSLVAYTCTDR"
     gene            complement(8246..9793)
                     /locus_tag="PK224_19085"
     CDS             complement(8246..9793)
                     /locus_tag="PK224_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17911.1"
                     /translation="MTGWFLPAPRLTAATVLQLIFALNCPPHTWGEEKVNPSARHEHL
                     AAAITEELKSISPSRANHQAYVHWLHLDRDDTVDALVIVKPNDSSCAVIKGCLGLVMR
                     GDKETFHLESVFAPNQHPIFLGPRKPDGSLRNFYLSQDGKDFVEFSHRIDHYKITQKG
                     LSYKKAQTLAPWAIGETQFDALSRQTALVRESDLQPAGAPMVLQIGVPPTSIHGQRKT
                     LSYGTTKALEGQIRSFLSQIASRILLSHTVVIELVACEDWIARIPLSDRGDRPLNLVV
                     ACQEILGLLHEKFPDTLLNDDGDHYQLIPKNKLDNWRALTAEQRSSAEQSAQAELVEK
                     YMKPAGLVPAPSISRENKLEALIFILAGSIGQPLALQQNIPEQLLAQMNTNNIEHDKY
                     TSRAITYVMNAPQFLYYLIGYRSLIQGSHHFFSYLAADEFFTRATLLIRYKKSLLQTN
                     KEQDLFMADIPARLWAFHCALRDRKNHESTGSEITSNRKTHTDQSDPDWKVCDQIQNL
                     LARISGS"
BASE COUNT         2335 a         2461 c         2758 g         2551 t
ORIGIN      
        1 gccccgacca aacaaagctt gctcgacccc gaatttcaaa tgtgcgcggg gatattcaac
       61 acctccagtg aagtcttttc ccgtttgctc gacccggagt ctcgcgatca agccgaatat
      121 agagccaacc agtaccaaga tgagcggtgt ctgttctttt ttgtaagggc agattcttct
      181 tagggcgggc gaatcgaaga aatggtgcta tttcggtccg tgaaacacct gctccccata
      241 tctcgcgaca atagcagatc agttccctac ccttataagt aaaaattgga cttcttgggt
      301 aaaaacgttg attcctctgg aagggaggag taccctgcgc gatgtttcgc tgtgcgcttt
      361 gctggttgct ctgcgattgc cctgtctgga gaacggtcag atgcgattca gagttcgatt
      421 ctgctcctca ctgactcttc ctgtgattgc ctcctgcacg ttcgtccggt ttcaggtcct
      481 cggaatcccg actactgttt atgatttgag cggaagtgac tggggcatcg ttccctgtct
      541 tcatcaagat ttcgaggtct cgaagccgtc tacgtattct ccaggccatc gacctgacag
      601 tctctcctct gtcatttcga gttgtatctt ccggtgtcgc aaaaggggac gtaatgtctc
      661 ttcaattcca tttccagatc actcttccgt tccatgtctg tttggggcag gacctgtgag
      721 cctttcgcaa ggaggttgct catgaaaaag atacccgcgc tatcattggc agcatgcgtc
      781 gcttggcaga tggcatggtc accttcctgg gctacgcctg cttatcctga gccgtccgaa
      841 caggaaatgc gacaggcgat cgaacaagcg atggttcaac gaggcggcag ccaaacccgt
      901 cccggtgaaa tcagcgtcga taaccccatt aatggtatga cgatgaccct cacggcgttt
      961 acgaagttag gctgtgagcc ggcgaatagg gggccagggt atgtctgctc gtatcatgtc
     1021 aagacgaaga tgagcgctca ttcgaatgaa gggtcgcgac agggggataa tcatgccgcg
     1081 gcggtcaatc aattattgcg cgccatgatg gggggacaag aggaagtggc tgataccgcg
     1141 acccgccgct tcctgcgaat gggagatcaa tgggtcatgt cgagggagtg acggaggagg
     1201 attgtcggca gctatagaag aaaaagtgcc cgcgatgtcg attcctgacg cattgatcca
     1261 tctgctgagg tgggcggctc gacgcagtca tcgtttacct cactgttctc cgctttcgca
     1321 gtttcgaggg aggtggagac cagacgtcaa gtctgggcgt ggccaacgct gccttgtgtg
     1381 ctctggattt tgaggattct cgtttcgtta gttgcgcgct gtcggttgac agtagcgggt
     1441 tccgtcagcc tacagatatt cgcctatgtg aagaccatga ccccgcacgc tgtggtacag
     1501 actgtcactc tccacgattg atagcgatct gtaagccgaa aaggtaaaaa cacggttgga
     1561 atgccgtgga atgaattcag aagttcaaga tatgtaccgg agtttattta ttaagcaaca
     1621 gtatcagcgt gtcggatcga atatgctgta cggatttctt gttgtcggca tcctgctctg
     1681 tgggaccttc ctccctttca ttgctgtgca aaacgttacg gccgaagtaa atctcacaga
     1741 tgggaaagat ggcatcacat ctagtgggac aaacctcgaa ggacagatct ccatagtgta
     1801 tcaggggaag aacagtctgg gcaccgttca tgaacagcag gtcgcagaat tggccgagcg
     1861 cctacgagca cgggttacgc cacctcctgg cccattcagc acgattctct ttgagaagct
     1921 ggtgaaggta ttaacgtatt ccgatcctgc tcacgcagag gatcgtgaac ttatcaaaaa
     1981 tgtccaagag ttcttggacg gcaaaataag ggactcgctg tttgtggcct atttggagga
     2041 agtgttgcca aaagaggtgc ttcctcctgt tcaggcgaca caagcgctga cattgttgca
     2101 ggaaaaggta aaaaacggag atttatgggc catgttctat cttgctcgtg caaatttcca
     2161 tgcggcgaat aatagccgag tcatacaatt gcaggagcag ttcgagggcc ttgcgttgcc
     2221 ggaggccacc cgagagtatt gggacgcata tcagcagctt ctccaggata agaattttcc
     2281 aggctcattg gaagggtgtg tcaaactggc aaaagaaggt caaagtaagc ggttcgctcc
     2341 gctcatcaat atcctgggag ttcagacgat gttggggctt ggagttcaga aagatgaggc
     2401 acttgcattc aaattgttcc gtgaagcggc caatgcagga tttgcaatgt ccatgacggc
     2461 tgtggcctct cagtatcttc agggaaatgg agttcaaaaa gatcaggcag aggctgttcg
     2521 ttggtatagg aaagcggccg aatccggtta tgcaagtgca atggcgtcac ttgcagacat
     2581 gtatgcgcaa ggcgcaggag tcggtaaaga cgaaagtgaa gcagcgaaat ggtttcacaa
     2641 agcggctcac ttgggaattt cagatgcgat ggtgtcgttg gctacagcgt atgagttggg
     2701 cagaggactc atgaaggatc agggtgaggc tgttaactgg taccagaggg ccgcggatcg
     2761 aggaaatgct cagggaatgg cggatttggg cagaatatat acatatggga tcggaatcga
     2821 caaagatttg aaacaaggaa ctaactggct tcaaaaagca gcagaactgg gaaattctga
     2881 tgggatgacc caacttggat acgtatatgc tgaaggcgtg ggcgtggaaa aggacccatc
     2941 ccgagcgatg cattggtata gaaaagcggc tggattaggc catactcaag ccatggtgct
     3001 tttagggggg atgtatgagc aggggcgcgc agtgccagtt gatgaaaagc acgcggtcgc
     3061 cttgtacgaa caagcggcga aagccggcaa tgagaacggg cgccaagcgt tcgaacgatt
     3121 gaaatccgac atcgagaaac gtgggactca agcggtccgc gaatgggccg accgatatca
     3181 gagggctcct aatatttcgg ggggggaatt tctcaagctg gtctcatctg cgaaggtaga
     3241 ttttcggttc acaacgaact ttgagaaata cgtagatgcc gaaaatatga ctcgacgagt
     3301 gcgtcaagcg atcgaaaaag agggcattcg aattgtggaa gatgctccga tcaccattcg
     3361 tgtagtctgg tacttgaacc ttcaaaccat ccagacgacc gggggcgggg ggagagaggc
     3421 gcatccaaac tatctctacg ctgaaacgat tgattttgaa gtagtgtcgc ccctgtatag
     3481 ggacaagagc tttcacaatc tgcgagttgt gccgctcaca gcattttatg caggcacggc
     3541 cttgtcaggg gagtgggttg tggacaaaac gatccgagaa acggtggtcg ttgacctgtt
     3601 gaagggaata gcagaagagt tctcggcatt gcgatctcca gatgataggg gaaaagacac
     3661 tgcgaggacg aatcaggttt cctggagtgc tgaagcaaac gagtccatgt atcgacagta
     3721 ccagatggcc aaacaccaag caggcgatga tgtagagcgt gtttttaagg gattgacatc
     3781 ggtttcaagt atcacgacaa ggctgacggg gaaagcaggt aagttccttg acgaaacaga
     3841 tgtcaatcgt gtttggaagg ttggacttca agaggccaga ttggagcagg caagtgatgg
     3901 agccctttgg gttgctcata gaattcacct cgacaaagaa aacgggtcga agttctcgct
     3961 attgttcggg aatctgagca gtatctggat ggggcctatg ctctcctgga ttacggtagg
     4021 agagagaggt acggtatttg agtttgctgg aaaattgttg cggatggatg ttgtgttgtg
     4081 gaccgacatc cggtacacaa gcgaattctg ggaggaagtt ggaagcaaac ttcccgagtt
     4141 cgttcatgga cgtattcgag actgtgtcaa ggagacacgg ttcagaagat aaacgctgaa
     4201 gggtgcaact gtcgacagaa tcgtgtgctg tgttggtgac cttcccccat ttgttacacc
     4261 accgccaagg aagttgactg ctgtgccacg tgggccccac cttgataggt gttgatgaac
     4321 tcctggggtg tcacatcccc aatggtgctg tgcgtccgct cctcattgta ttcccgtcgc
     4381 caggcttcaa tcacgagctg cgcttcctgg agcgtcagaa accagtgctc gttcaaacat
     4441 tcatcccgaa atttgccgtt gaagctctcg ataaacgcat tctggactgg tttgcccggc
     4501 tgaataaaat gcaggtgtac gccgtgctga gcagcccagg catccaacgc catgccggcg
     4561 aattcagggc cgttgtcgag gatcacggtc tccggcaacg gacgcgtggt aaacagctga
     4621 ttcagaatgc ggcacacccg ggcgccccca atggagacgt cgacttcaat caccggcact
     4681 tctttcgagc agagatcggt catcgtcaaa cacttgaacc gtcggccatt ggccagtcgg
     4741 tcatggacaa aatccatcgc ataacagcgc ccgggttgcg tgggtgttgg caaggcgacc
     4801 cgtggcaccg ctgcggcctt cttccgtcgg cgtcgccgta acgacagccc ttcgtcgcga
     4861 taatagagcc gttccacctt cttatgattc actcgccaac cttcccgtcg caggcgcaca
     4921 taaatccggg gacagccata ccgcctcttg gcttccgcaa tctcccgaat ccgcgtccgc
     4981 agggcggcat cctcctgacg ccgactgcgg taccgcagcg tgttccggtc caattcgagc
     5041 aaccgacaga cccgtcgctg actcagccca aagcgctcgg ccatccactg cgccgccgtt
     5101 cgcttcgcct tgggccctac cagttttttg cggtgatcgc cttcagcgct tggatgtcga
     5161 gcgcttgctc cgccaccatc tgcttcaacc gccggttttc gtcttccagc tggcgaagct
     5221 tcttcacgtc gctgacttcc agccccgcat acttggtccg ccacttataa aaggtcgcat
     5281 ccgagatgcc gtgcttccgg cagagctcgg ggactccgat gcccgcctgg gcatccttga
     5341 gcactgcaat gatttgttcc tccgtgtgcc gttttcgtgt catggccccc tcctctgagg
     5401 cccatctcag cacagttgga cttcctagcc aatggtctag ttttcggggg gaaggtcatt
     5461 ggtattggct tgcgctggtc ttactctgag gcataagagc gtgccggttc agtgataacg
     5521 gcaaaatcgc tcagttcctt gtccgacatg acttgtagac tcttgatggt cgggtcaaac
     5581 atgtgatcga gaaatttgct tgagactctc tgcttttgta cgaagcggta atactctaaa
     5641 acggttttct gagtgttgcg ttcaatctta gtgatattgg tactaagttg gtctgcaagt
     5701 gcactaacca atttttggat ctctggagag tttagcacct gagcatattt atcgcgctct
     5761 cccttaggcc aggcccaccc ttcctgtgtt gtctctggca ctggaatccg agtgaggagt
     5821 gatgctgttt gcccagactt catttgagcg ctcaaagtat gcaatagttc caatgtgagc
     5881 aagacgcttg cgttgtccgg aattccagtg taagtaaaac cactatttcc atcatacttg
     5941 taggccgaat aggcatgtac ttcgtaagtg ccatctaagt ccttcgttct tcgccgcccg
     6001 cccaagaaca ctaaggtgca ggcgctgaca caacggctgc cagctggaac atgagttctg
     6061 aagccatact tcgaaatttc ttctgccata ataaatgcgg ccttgacaga gccgccagtg
     6121 gaatcaaggt caattcttcc aatttttccc tgcttgtcga ggcacgactt tagctctctt
     6181 tggaaggggg ggccgtcctc gtattctatt ttcccagtaa aggaaaatat tccagtatta
     6241 ggctccttgg ggtcagggct gcaagcaaac ttacttcctc ccgcggccgc atattctatt
     6301 gcgacaaaca attgaacaag aataagcaca aagtggacca ttgtcgctcc ttgcaataga
     6361 agaagaggtc atgcccgttg taattcaatc cgttgccagt gcccgtccga agctgggcag
     6421 gcattggtct cttgcgatgc ttcaacacca tccttcataa ctatctcttt tattgcgaca
     6481 cagccttttc cgccagctgt gcgcatgact tcacctgttt ggggagactt tgtaggacca
     6541 atataaattg cttgtccttt tgtgctccct tcgctccagg actccgggct actctgcttt
     6601 gactgcgcgg cttcaatctc acgctgtctg gcttgctcac gggagattct gtctgcatca
     6661 gtagcggggg caggcatgtt ccgataccgt tcctcgacct gtaaacgctc tcgttcggcc
     6721 tgctcggcgc ctgcctgtgc agccttctgg aagacctcat cctggcgttg ctgttctcgt
     6781 tccgtgagat atttcccgat cgctgtggcg gccaggccga ctgcgcatcc agccccaccg
     6841 ataagagcgg cctttttcag actttcactt gtcggggttg ggacgcgact tcctttttgg
     6901 gatcctgctt tcaggatatc ccaaagaaga cttcctcccg cacccagagc tgcgcctgcg
     6961 acacaccccc cgacgtagct agagtttgac tgacctgcct ggttgggttg cccatacatc
     7021 ccttggcagc ttgacaacag caagcatagg ctcgctaccg gcgctatggt tcgcatggaa
     7081 ccatttctgt ttctgttcga tcgagaaaat tgaagagcca tgggtacctc cttgaatgaa
     7141 ttaacgatcg gtacatgtat aagcaaccaa agattttgat tccataccat tcgaactgga
     7201 ttcgcgaagg cggttcagtt cctcttcagc gtgagctaac ttggattcgg cattcctaag
     7261 gtcgagactt agttgcttat gattctctcc aatcgcctga cactgcttaa gaaggcgctg
     7321 aggggttgct cctgcttcag caactttggt ttcccactgc tttgtgtgtt cgataattcg
     7381 ttgaatggca gctttttcgc actcagccgt tctggtatca aacaacgaga gaaactcatc
     7441 gtgctggaga aagcgttcgc cttgagctaa ggtaagctcc catcctgcta tctcgctgga
     7501 atcaggtgtc atatggatgt tcagtgcgcg ctgtacgtcg gccgcctgca cacgctgatt
     7561 ccatgcgtct ggtctttttg tagcggcgta caattttcct gccatttctt taagcggtcc
     7621 ttcccatgct accgcgcagg cttgggcagc aacgatgggc cgattctctg gcccaaccgc
     7681 tgtaacaggt cttggtggaa gagggactgg aggccccact ttgcactcat caagagaccg
     7741 aaccccatag ccttgaagct cattaaacac cacatcgagt cttgccctca tcgatgtggc
     7801 ttcagcacga agggaaccca cattctgact cacttgcttg cgagactgca ctctcgcaag
     7861 atcttcttcc cataacggat ttctgaatgt gtgcggctcc gagtgattgt tgcggactgg
     7921 gacttgtccg tgtggtccta cgattgcgag gcagattgga gggcttactt ggatagtgtc
     7981 agccgtcggc aaggtgaagc ggagcgttcc attcttgggg tcggcaacgg cctggcttag
     8041 tgcccttcct ctgaagacat ggctatttag gtcacttgct cgaatgagtg aatatgtccc
     8101 ttgcagacgc gaattgggag cgccatctgg tcgaaagatg atcgtatgtt tggttcccgt
     8161 gagatctgac gtagcacagc cacccaagac taggcacaaa gacaaaggaa gccaaagccc
     8221 tagaaggcga aaacccataa gaacctcaac tgccagaaat tctggctaat aggttttgta
     8281 tctggtcgca cactttccag tctggatcag attgatcagt atgagtcttt cgatttgacg
     8341 tgatttcaga ccctgttgat tcatggttct tacgatcacg tagggcgcaa tgaaaagccc
     8401 aaaggcgagc gggtatatcg gccatgaaca ggtcctgttc tttgtttgtt tggagtaaag
     8461 acttcttata ccttattagt aaagttgcac gagtgaagaa ttcatctgct gcgaggtatg
     8521 aaaagaagtg atgtgatccc tggatcaggg atcgataacc aatcaggtag tacagaaatt
     8581 gaggcgcgtt catgacatag gtaatcgctc ggctcgtata cttatcatgc tcgatattgt
     8641 tggtgttcat ctgcgctaag agttgttcgg ggatgttctg ttgaagggcg agaggttgtc
     8701 ctatggaacc tgccagaata aatatgaggg cttctagttt gttctcgcgc gagatcgaag
     8761 gtgctggcac gaggccagcg ggcttcatgt atttttcaac cagttcagcc tgagctgact
     8821 gttcggctga ggacctttgt tcagccgtta gggcgcgcca gttatccagc ttatttttag
     8881 ggataagctg ataatggtcc ccgtcgtcat tcaagagcgt atcaggaaac ttttcatgta
     8941 aaagccccaa gatctcctgg caagccacca caaggtttag tggcctgtcg ccacggtcac
     9001 tcagcggaat gcgagcgatc cagtcttcgc aagctaccag ttcaataacc acagtgtgag
     9061 acaagaggat gcgtgacgcg atctgtgaca ggaaggatct gatttgtccc tcgagtgctt
     9121 tagtcgtccc ataactaagt gtctttcgtt ggccgtggat agacgtcggc gggacgccga
     9181 tctgtagaac cataggtgct cccgctggct gcagatcgct ttctcgaaca agggcagttt
     9241 gtctggagag tgcgtcaaat tgtgtctcgc cgatagccca tggcgcgagc gtctgcgcct
     9301 tcttgtagga cagtcctttt tgggtgattt tgtagtgatc gattctatga gaaaattcga
     9361 cgaagtcttt tccatcttgg gagaggtaaa aatttcggag ggaaccatcc ggctttctgg
     9421 gccctaagaa tatcgggtgc tgatttgggg caaagacgga ctctaaatga aaagtttctt
     9481 tgtctccgcg catgacgagt cccaaacacc ctttgatcac tgcgcaggag ctgtcattcg
     9541 gctttacaat aaccagtgcg tcgaccgtat cgtcgcgatc gaggtgtagc cagtgcacgt
     9601 acgcttgatg gttggcgcga gatggggaga tggatttcaa ttcttccgtg atagctgcag
     9661 caagatgttc atgacgagcg gaaggattga ccttttcttc tccccaggta tgtggggggc
     9721 aattcagagc gaatatcaat tgaagtactg ttgcggcggt gagtcgcggt gctgggagaa
     9781 accaacccgt cacaattaat ccgttcggtt ccaacaggat gaagggaatg gcggcgggat
     9841 tatacgtgaa tgaggacagc gaatgaaagc ggattggaat gagtcaatta ggaccggacg
     9901 gggttaatac gtcctatagc gaatacacgg attgggagtg attccaacta gcgatcagta
     9961 ttgaatgaga tgttccagcc gtaaatgaac tcgaccgtaa tggttgtatt cttgctaatg
    10021 gtgggtgtta tgaacctatc tttcggacgt ctgaccttcc cccgatttca cagacacctg
    10081 tataaggagg aatgtgatga gatcg
//
LOCUS       DAORRQ010000100        10067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_300_length_10067_cov_17.441670, whole genome shotgun sequence.
ACCESSION   DAORRQ010000100 DAORRQ010000000
VERSION     DAORRQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_300_length_10067_cov_17.441670"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PK224_19090"
     CDS             <1..715
                     /locus_tag="PK224_19090"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRB17912.1"
                     /translation="AHTDFLIGNDYVLKTAQAHPTEFLAGVSINPQRRDAIDEVHRCA
                     DAGAILVKVLPNAQQFDPADRKYTPFYRALAERKLPFLSHVGYEFSLIGKDQSVGEPD
                     RLRLALEEGATVIAAHACSYGLILYEKFLPTLRDLVTRYPHFYADISALTLPNRMRML
                     LHLRQYPELHERLLFGTDYPLSVFHMAAWGRVALGTLRKMIGTKNRFDRQVEVCNGLQ
                     LGFRSLGDLLPHSSGMAGH"
     gene            727..1224
                     /locus_tag="PK224_19095"
     CDS             727..1224
                     /locus_tag="PK224_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB17913.1"
                     /translation="MTRDEILTSLRERILAFATSRVSREVAEDLTQEVLVLLHEKYAR
                     VTELTELVPLAFQVLRFKMLDAHRKSLRRGEYNQESVDELPLANPGDDPATLLDQKQR
                     VDRLLAAVAQLGDRCRDLLTLKLEGHSFPEIQTRMGQHSINTIYTWDLRCRKQLLSLM
                     GGTWE"
     gene            1279..2619
                     /locus_tag="PK224_19100"
     CDS             1279..2619
                     /locus_tag="PK224_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17914.1"
                     /translation="MPEHDLEKLLGGFAANTLTPEERSQLYTASLQDQQLFNALADEQ
                     ALKELLSDPAVRRKLLEALKRPNTAEAGGSLPWWAWFKRPAGLAFAGGLAAVIFALAF
                     GTRIYQESLDRAAQSVATEDARQAGPSVRTPPSADSPRPPDAESQLKAKAPGEPAQAP
                     AKKDVLTDTAAKRERPAAAKLPEQRAAETASDSAMPLRERDETRKQAQAPVAAPGRTA
                     EQAAASTDKKLSPGVQAPPATPAPAVAPAASVAAPKKSARALFYGEPSPRADTGSLAQ
                     ESKQAKQLGQVKPLGLRYSFVTRGLDGQSQEVNAATVGASKEPMRITVETSQDAYLQI
                     LQNLGSAGTRLWWPPQETGKISLKLLAGKRSEIPLPPPAENGLLALTIRLSPKPFGPL
                     TMQEVGMLDRFSANLIIESVSPADTSGAQEQATYVVNPEASPMAQLAVDIPISQ"
     gene            2714..2986
                     /locus_tag="PK224_19105"
     CDS             2714..2986
                     /locus_tag="PK224_19105"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRB17915.1"
                     /translation="MPIIKPVSDLRNKTNQISDLVHKSHEPVFITKNGEGDMVLMSLA
                     TYGEIERTLDLYGKLAVAEAETAKGGRGRALSQVMADLRKRIRAFK"
     gene            2973..3287
                     /locus_tag="PK224_19110"
     CDS             2973..3287
                     /locus_tag="PK224_19110"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB17916.1"
                     /translation="MRSNKYSVRLLSVAGQDLQELLSYLAAENPTAAATALDRIESRL
                     HALEHHPLLGRTPNDRTVAGLGYRVLVIDNYLAFYKVKGKTVLIHRVLHGARDFVRLL
                     MD"
     gene            complement(3332..3694)
                     /locus_tag="PK224_19115"
     CDS             complement(3332..3694)
                     /locus_tag="PK224_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17917.1"
                     /translation="MLELLLVLGTVVAIALIGLVTVAMTPPLMVELGLWGLAVGLFIG
                     IPTGWWYHVVLYRTLTARMALPPRWWGRPVELHPLLAPAEYQGVRPWFVAGALGFFLC
                     LAGGVAAISGLLVLRFYP"
     gene            complement(3845..4840)
                     /locus_tag="PK224_19120"
     CDS             complement(3845..4840)
                     /locus_tag="PK224_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB17918.1"
                     /translation="MLFITSRMPTVNTEPELNPNFVFDLRNNSSSRGFFCCNRNKNGA
                     IEEIGSKNFLTAIKESQYRQVIIYIHGFSNLPEDVFNDAEEFQALCNKEKNGELLVVP
                     IIWPCDNDLGLVKDYWDDQKAADQSAFAFARMFQKFMEWRNSADANPDEDPCLKRINV
                     LAHSMGNRVLRQTLSLWQKYDQPGGLPLLFRNTFLIAADILNESLHKGEAGEPISHAS
                     RNVVVYYASDDLALRASKVSNLKNAEASRRLGHTGPEDMDLTPKNVYAIDCDDVNTVY
                     DPPKGHSYFRSGRRKGHPGVVFDHIFETVLTGRVFPNDESRKSSILELPRSRSKA"
     gene            5420..5839
                     /locus_tag="PK224_19125"
     CDS             5420..5839
                     /locus_tag="PK224_19125"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRB17919.1"
                     /translation="MKSTEPQPLGDIDLKLKDILSRHPSVEMAFLFGSLAAGQGRMGS
                     DLDLAVATVTPLTPQTKIELIEDLAVAIGRPVDLIDLAQAHGPLLQQIVTRGRMVLCK
                     NRTLYAELLRRVAYDEADVMPYYRRILSERRQAWIGI"
     gene            5824..6243
                     /locus_tag="PK224_19130"
     CDS             5824..6243
                     /locus_tag="PK224_19130"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRB17920.1"
                     /translation="MDRNIVEAKLESLRRCVDRIAEKTPASADQLIHDPDLQDIIALN
                     LQRAVQTSVDLAAHLIAESDAPAPSTMAEHFEILDHLQIISPALAQRIKKAVGFRNIA
                     VHSYQAIDWSIVFQISRHHLDDFKQFAQAVTKRLSAS"
     gene            6629..7480
                     /locus_tag="PK224_19135"
     CDS             6629..7480
                     /locus_tag="PK224_19135"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRB17921.1"
                     /translation="MEHIEGKSQSNPSANRRDPRLYHIASLGTLLAYGLFWLHFDLSL
                     WQIAVTIGTALLTQYAGTRYAKLPCFDPKSALISSLSLCILLRTDHLAIEALASVVAI
                     GSKFVFRWNDKHLFNPTNLALAIALASGMGWISPGQWGQVAWFGFLIACLGSLVVTRA
                     ARADVTFAFLAVYVSLLFARALWLGDPLTIPFHQIESGALLIFAFFMISDPKTTPDSR
                     IGRIIFALCVTLAALYVQFGLFRPNGPLWGLIACTPLVPVLNQLFPGARYVWTRPSTG
                     SASPNSS"
     gene            7562..8908
                     /locus_tag="PK224_19140"
     CDS             7562..8908
                     /locus_tag="PK224_19140"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HRB17922.1"
                     /translation="MRRTIVPSLLFALGLLAWSGEALAFCGFYVGKADTRLFNKASEV
                     AIARHENKTVITMANDFKGDVKEFALVVPVPTILEKEQIHVGNPAVLNHLADYSAPRL
                     VEYFDENPCLRYELMERRSMDAMKSMTPAAAPARERDKALGVTVEAQYTVGEYDILIL
                     SATESNGLESWLTENGYRIPNGASAVLHSYLKQGMKFFVAKVNLGEQTKLGLTHLRPL
                     QIAFESSKFMLPIRLGTVNADGAQELFIYFLTRQGRVETTNYRTVRLPEAQELPLYVK
                     DKFGDFYRDLFTQQVKREHERGLFLEYAWDMNWCDPCAANPLTADELRSLGVFWQDGH
                     GWTGKGMPAAQNVFLTRLHVRYDAAHFPEDLMFQETSDRGNFQARYILRHPWTGTDEC
                     AAATAYRHQLRERYEREAQTLASLTGWNIGDIRKTMNIASVPVGEEKKWYQRLWTN"
     gene            complement(8997..9437)
                     /locus_tag="PK224_19145"
     CDS             complement(8997..9437)
                     /locus_tag="PK224_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HRB17923.1"
                     /translation="MKTRVLLYGLVGVTLGVAPVRAESVPMKPGEYQVTAVTDASNGE
                     AGKPDTRTRCVREEHLANPDAVFNYYALNGFQPNPSSKVVNVSMHGGKVSYDIEGVYA
                     VTRVEGTVSPTGFSVVRKATPKGDHALSVTMKVDAKRTGDCPGK"
     gene            9691..>10067
                     /locus_tag="PK224_19150"
     CDS             9691..>10067
                     /locus_tag="PK224_19150"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HRB17924.1"
                     /translation="MIANHRPIVAAFGGVSLVLLLSACSFKATFKETTDTTSNITGTT
                     SGRIWWNEDGLLKPEHKVAAFTAYNADNLQSDAARGQGEYLASLDRLMGASDASVFQP
                     FAQDSFSRWSRTGSTSTTDLVNDL"
BASE COUNT         2152 a         3152 c         2775 g         1988 t
ORIGIN      
        1 ggcacacacc gactttctca tcggcaacga ctatgtcctc aagaccgccc aagcccatcc
       61 cactgagttc ctggcgggcg tctctatcaa tccgcaacgg cgggatgcca tcgacgaagt
      121 gcatcgttgc gccgacgcgg gcgcaatcct ggttaaggtc ctgcccaacg cgcaacagtt
      181 cgacccggcc gatcggaaat acaccccctt ctaccgcgcc ctggcggagc gaaagctgcc
      241 gtttctcagt catgtcggct acgaattcag cctcatcggc aaggaccagt ccgtgggcga
      301 accggaccgg ttgcgcctgg cgctggagga aggcgccacc gtcatcgcgg cccacgcctg
      361 cagctatggc ttgattctct acgagaaatt tcttccgacc ttgcgcgacc tcgtgacgcg
      421 ttatccgcat ttctatgcgg atatttccgc cctcaccttg ccgaaccgaa tgcggatgct
      481 tctgcacttg cggcagtatc cggagctaca tgaacgcctg ctcttcggca ccgactatcc
      541 gttgtcggta tttcatatgg cagcctgggg ccgggttgca ctcgggacat tgcgcaagat
      601 gatcggaacc aagaaccgct tcgaccggca ggtcgaagtc tgcaatggcc tacagctcgg
      661 attccgctcc ctcggagacc tacttcctca ttcatcagga atggcgggcc actgagtatt
      721 cttgccatga cgcgagacga gatactcacc agcctgcgcg aaaggattct ggccttcgcg
      781 acatcacgtg tatcgaggga agtcgccgag gatcttaccc aggaagtgtt ggtgctcctg
      841 cacgagaaat atgcccgcgt cactgaactg accgaactgg tccccctggc ctttcaggtg
      901 ctccggttca aaatgctgga tgcccatcgc aagtcactgc gccggggcga atacaatcag
      961 gaatcggtcg atgagctgcc gttggctaat cccggtgacg atccggcgac actattggat
     1021 cagaagcagc gtgtcgatcg actgctagcc gccgtggctc agctcgggga tcgctgccgc
     1081 gacctcctca cattgaaatt ggaaggacac agtttcccgg agattcaaac gcgcatggga
     1141 cagcattcga tcaacaccat ttacacctgg gatctccgct gccgaaaaca gttgttgagt
     1201 ttgatgggcg gcacgtggga atgatgatgt cggccctcat ttcacgtctt acgtttcacg
     1261 tattacgcag taacagacat gcctgaacac gatctcgaaa aattgctcgg tggattcgca
     1321 gcgaatacgc tgacgccgga ggagcgctcg caactctaca cagcctccct gcaggatcag
     1381 caattattca acgccctggc ggatgaacag gcgctgaaag aattgctgtc cgatcccgcc
     1441 gtgcgtcgga agctgctgga ggctctgaag cggcccaaca cagccgaagc cggtggatcg
     1501 cttccctggt gggcatggtt caagcgaccg gccgggctcg ccttcgcggg tggtctcgcc
     1561 gcagtgatct tcgcgctcgc attcgggacc agaatctatc aggaaagtct cgatcgagcc
     1621 gcccaatcgg tcgcgactga ggacgcccgg caagccgggc catcggtccg aacgcctcca
     1681 tcggccgact caccccgacc gcccgacgcg gaatcacagc tcaaagcaaa agcgccaggc
     1741 gaaccggcgc aggctccagc gaagaaggat gtgctcaccg acacagcagc caagcgagag
     1801 cgacctgcag cggcgaagct gccagaacaa cgcgccgctg aaacggcgag cgacagcgct
     1861 atgcctctac gcgaacgcga cgaaacgcgg aaacaggccc aagcccccgt cgcggcaccg
     1921 ggcaggacgg cggagcaggc agcagcatca accgacaaaa aactctcgcc cggagttcag
     1981 gccccgcctg ctactccagc cccagcggta gcgccggctg caagtgtcgc cgccccgaag
     2041 aaaagtgccc gcgccctctt ctatggcgaa ccgtcccccc gtgccgatac aggcagtctc
     2101 gcgcaagaga gcaaacaggc caaacagctc ggccaagtga agccgctggg gttgcgctac
     2161 agctttgtga caagggggct cgacgggcaa agccaggaag tgaatgcagc aaccgtcggc
     2221 gcaagcaaag agccgatgcg catcacggtg gagaccagtc aggatgcgta tctgcaaatc
     2281 cttcaaaatc tcggctctgc cggtaccaga ctgtggtggc caccacagga gacgggcaag
     2341 atctctctca agctgctggc ggggaaacga agcgagattc cattgcctcc cccggcggag
     2401 aatggcttac tggcgctgac gatccgtctg tcaccgaaac cgttcggacc actgaccatg
     2461 caggaagtcg gcatgctgga ccggttctcg gcgaacctca ttattgaatc ggtttctcca
     2521 gccgacacgt ccggtgcaca ggagcaggcg acctacgtcg tgaacccgga agcgtcaccc
     2581 atggcccagc tcgccgtcga cattccgatc agtcagtaac ccgagagcta cccacacgtt
     2641 ccttccagtc cttttccatc tttctcatat catgatacag tatgaatcat gatagacatc
     2701 gggagggcca ggaatgccga tcattaaacc tgtttcggat ctgaggaata aaaccaatca
     2761 gatttctgat ctggtgcaca agagccatga accggtcttc attaccaaaa acggcgaagg
     2821 ggatatggtg ctcatgtctt tggccacgta tggagaaatc gagcgcacct tggacttata
     2881 tggaaaactc gctgtggcgg aggccgaaac cgcgaaagga ggccgaggac gtgcgctcag
     2941 ccaggtgatg gccgacttgc ggaagcgtat ccgtgcgttc aaataaatac agcgtccgac
     3001 tcctctcggt cgccggacag gatctgcaag aactcctttc ctatctggcc gcggagaatc
     3061 ctactgcagc ggccacagca cttgaccgca ttgaaagccg gctgcatgcc ttagaacacc
     3121 atccactcct aggccgaacg cccaatgacc gaacagtcgc gggactgggc tatcgggtcc
     3181 tcgtcatcga caactatctc gccttttata aagtcaaagg aaagacggtc ttgatccacc
     3241 gcgtcttgca tggcgcacga gacttcgtgc gtctgctgat ggattgattc cacccgtcag
     3301 gcgcccccgg gagattgccg ctgcccccgc atcacggata gaaccggagc accaacagcc
     3361 ctgagatcgc cgccacacct ccggcgaggc acagaaaaaa ccccagcgcg cctgcaacga
     3421 accagggtcg tacgccctgg tattcggcag gcgccagcaa tggatgtagt tccaccggtc
     3481 tcccccacca gcgaggcgga agcgccatgc gcgcggtcaa tgtccggtag agcacgacgt
     3541 gataccacca gccggtcggg ataccgatga agagcccgac agccagcccc cacaatccca
     3601 gttcaaccat gagaggtggt gtcatggcga ctgtcaccag gccgatcagt gcgattgcca
     3661 caacagtccc gagcacaagg agcaactcaa gcatgggatg gattataccg gacaagttgc
     3721 agtgccggag ggtaatgaaa gagtacccgc caggacggaa tcagcctact ccggcttcca
     3781 tgccacaacc ggccacgctt ctccccgctt tcgccatgac catctcccgg cccagggatc
     3841 cagatcaggc cttggacctc gatcggggca attccaaaat actcgacttc ctggactcat
     3901 cgttcggaaa cacgcggccg gtcaggaccg tttcaaagat atggtcgaac accacgccgg
     3961 ggtgaccttt tctcctcccg gatctgaaat atgaatggcc cttcggcgga tcgtacaccg
     4021 tgttgacgtc atcgcagtcg atggcataga cgttcttggg tgtcagatcc atgtcttccg
     4081 ggccggtgtg cccgagacgg cgagaggctt cggcgttctt cagattcgag accttgcttg
     4141 cccgaagcgc caggtcgtcc gaggcgtaat agaccaccac gttgcgcgag gcatgactga
     4201 tcggctcgcc ggcctcgccc ttgtgcagag actcgttcaa gatatccgca gcgatcagaa
     4261 aggtatttcg aaacaggagc ggcaatccgc ccggttgatc gtacttctgc cagaggctca
     4321 acgtctgacg gagcacgcga ttgcccatgg agtgcgccag gacattgatg cgcttcaggc
     4381 agggatcttc gtcgggattg gcgtcagccg aatttctcca ctccatgaat ttctggaaca
     4441 tacgggcgaa ggcaaaggcg ctttgatccg ccgccttctg atcgtcccaa tagtctttga
     4501 cgaggcccag atcgttgtcg cagggccaga tgataggaac cacgagcaac tccccgttct
     4561 tctccttatt gcagagggct tgaaactcct ctgcgtcgtt gaagacatcc tcgggaagat
     4621 tcgagaagcc gtggatatag atgatcacct gacgatattg ggactctttg atggccgtca
     4681 aaaaattctt gctgccgatt tcttcaatcg cgccgttctt gtttctgttg cagcagaaga
     4741 atccacgact cgacgaattg ttacgaagat cgaagacgaa gttcggattc agttcaggct
     4801 cggtgttgac cgtcggcatt cgactggtga tgaagagcat ggtggggtct ccttggtgaa
     4861 cggtgatcgg catagcccga cgcgcaagca accgtcaggc agcagccgct gatgtctgct
     4921 tgcagcattc tgtgtggcac aggtctatcc ccggcagcgc acgttgcagc ttgcgccgca
     4981 caggggaatc aaacctgtca tcggcgcggg gattctagca ggacagagga aattggcaag
     5041 acgagggata ttcgggaccg caaagtcacc ccctagaaaa attatcgaca cacttgcggt
     5101 gcttcagaag gagcacatgt tcgccctgat ctcctctccc gtcacggatt gaagaaatgg
     5161 gaatgaaagc tttcgctttc gctatggctc cggctgccga ccggtagtcg gacaaaacac
     5221 cgttgtgtcg ctctgttcgt ccgatgaggc ctgcgacgga caaaaacgtg tccaccacca
     5281 tccgctctgc attgcatcat gccgagaccg aagctgcccg ctattcgtca accgtggcta
     5341 ctgtggcagt gcccaggccc gccctgttct tgccactttg tcccattcgt gatactcaaa
     5401 gatgaggaac gaaccaacca tgaagtctac tgagccacaa ccattgggcg atatcgacct
     5461 gaagcttaag gacatcttgt ccaggcatcc ttccgttgaa atggccttcc tgtttggttc
     5521 gctcgcagcc gggcagggac gaatggggag cgacctcgat ctcgcagtag caaccgtcac
     5581 tccgctcacc ccacaaacca aaattgaact catcgaagat ctcgccgtcg caatcggtcg
     5641 tccggtcgat ctcatcgacc tcgctcaggc acacggaccc ttgttgcaac agattgtgac
     5701 gagggggcgc atggttctgt gtaagaaccg gacgctctat gccgaactgt tgcggcgcgt
     5761 ggcctatgac gaggccgacg tcatgcccta ctatcgccga atcctctcgg agagaaggca
     5821 ggcatggatc ggaatatagt cgaggccaaa ctcgaatcgc tgcggcgttg cgttgaccga
     5881 atcgccgaga aaacgccggc gtccgccgac cagttgatcc acgatccaga cttgcaggac
     5941 atcatcgcgc tcaatcttca gcgcgcggtt caaacctctg tcgacctagc ggcccacctg
     6001 atcgccgagt ccgacgcccc tgctccctcc acaatggcgg agcactttga gattctcgac
     6061 catctacaga tcatcagccc tgccctggcc caacgcatca agaaagccgt tggattcaga
     6121 aacatcgccg tgcatagcta tcaggcgatc gactggagca tcgtgttcca aatttcccgg
     6181 catcacctcg acgacttcaa gcaattcgct caggcagtca ccaagcgcct ttctgcgtca
     6241 tagcctctcg atctttcctc actgaatctc cgatcaacgc ccgccctccc cagcttgcct
     6301 ggaagagatg ggcacgcagc caaacggtcc ctgaaaaggc tattcgaggt agtggccagg
     6361 tggccctgca ctgcgcgtat gggacgcgca cgatttcatc gtgcgcgttc tgcgagcaag
     6421 aaagacagca gactgctcca atccttacga ccttccccca gctcccaact cccgcgaggt
     6481 ggcgggccta ggctctactg cgcgcgtcga gcgaggtcct tcctagggcg cgcgttccgc
     6541 gagcaaagag cctgggtccg ccgcctcgcc cctcccctct gaaaggattc ctcccttcgc
     6601 cccataccct cctgtgaagg gagggcacat ggagcacatc gagggaaaat cccaatcgaa
     6661 cccatcggcg aaccggcgcg atccccgcct ctatcacatc gccagtctgg gaacgttgct
     6721 ggcgtacggt ctcttctggc tccacttcga cctgagcctc tggcagatcg ccgtcacgat
     6781 cggaacagcg ttgctcactc aatatgccgg gacgcgttac gccaagcttc catgctttga
     6841 tcctaagagc gccctcatct cttccctgtc gctctgcatc ctcctccgca ccgatcacct
     6901 cgcgatcgaa gcattggcct cagtcgtagc catcggcagc aagttcgtgt tccgctggaa
     6961 cgacaaacat ctgttcaatc cgaccaacct ggcgttggcc atcgcgctcg cgagcgggat
     7021 gggctggatc tcacctgggc aatgggggca ggtcgcctgg ttcggcttcc tcatcgcctg
     7081 cctcggcagt ctcgtcgtga cccgcgcggc gcgggcggat gtgacgttcg cctttctcgc
     7141 cgtctacgtc agtctcctct ttgcacgggc cctgtggctc ggcgatccac tcacgattcc
     7201 gtttcaccaa atcgaaagcg gcgcgctgct catctttgcc tttttcatga tctccgatcc
     7261 caaaaccaca ccggactccc gcatcggacg aatcatcttc gctctctgcg tcaccctcgc
     7321 cgcgctttac gtgcagttcg gattgtttcg cccgaacggg ccgctgtggg gactgatcgc
     7381 ctgcacacca ctcgtcccgg tactcaacca gctgtttccc ggtgctcgtt acgtttggac
     7441 caggccatcg actggtagcg cgtcaccaaa ctcgtcgtga cacatcaatg cccaaccgtc
     7501 agccggtagt catccccagt ctgtctccgt ttctctcgtg tcaccaacca aggaggttct
     7561 tatgagacgt accatcgtgc cttcattgtt attcgccctc ggactgctcg catggagcgg
     7621 tgaggccttg gccttttgcg gcttctatgt cggcaaggcc gacaccagac tgttcaacaa
     7681 ggcctctgaa gtggccatcg cgcgccacga gaacaagacg gtcatcacca tggcgaacga
     7741 tttcaaaggg gatgtgaagg agtttgcgct ggtggtgccg gtgccgacca tcctggagaa
     7801 ggagcagatc cacgtcggca atccggcggt attgaaccat ctcgccgact attcggcgcc
     7861 gagactggtc gaatacttcg acgagaatcc ctgtctgcgt tatgaactca tggagcgtcg
     7921 gagcatggac gcgatgaaaa gcatgacgcc cgcagcagca ccggcccgtg agcgtgacaa
     7981 ggccttgggg gtcacggtcg aagcccaata cacggtcggc gagtacgaca tcctgattct
     8041 ctcggccacg gagagcaatg ggctcgaaag ctggctgacg gagaacggct atcgcatccc
     8101 gaacggggcg tcggccgtcc tccatagcta tctcaaacag ggcatgaaat ttttcgtcgc
     8161 caaggtgaat ctgggcgagc agacgaagct cggcttaacg cacctccgac cgcttcagat
     8221 tgcattcgaa tcgtcgaagt tcatgttgcc gatccggctc ggcacggtga atgccgatgg
     8281 agcgcaggaa ctgtttatct actttttgac caggcaaggc cgggtggaaa cgacaaacta
     8341 tcgaaccgtg cggttacccg aggcgcagga actcccgctc tacgtgaaag acaagttcgg
     8401 agacttctat cgcgacctgt tcacgcagca ggtgaaacgg gaacacgagc gcggcctgtt
     8461 cctggaatat gcctgggata tgaactggtg cgacccctgc gcggccaatc cgctcactgc
     8521 cgatgagctc cgcagtttgg gcgtcttctg gcaggatgga cacgggtgga cgggcaaggg
     8581 catgccggcg gcgcagaacg tgttcctcac ccggctgcac gtgcgctacg atgccgcgca
     8641 tttccctgaa gacctgatgt tccaggagac ctcggatcgc ggtaattttc aggcgcgtta
     8701 tatcctgcgc catccctgga ccggaaccga tgaatgtgcc gccgccacgg cgtaccgcca
     8761 tcaactgcgg gagcgatatg agcgggaggc gcaaacgctg gcgtcactga cgggctggaa
     8821 catcggtgac attcgcaaaa cgatgaacat cgcctcggtg cctgtcggcg aggagaagaa
     8881 atggtatcag cgcttgtgga cgaactagcg ggagcaggcg aaacgcagga ggccgggaca
     8941 acccggcctc cgcatcagca ggcatatcgt agcttcaacg cgcaacctga acggcatcac
     9001 ttgccgggac aatccccggt tcgtttcgcg tcgactttca tggtcaccga cagcgcatga
     9061 tcgcccttgg gcgtcgcttt gcggaccacg gaaaacccgg tgggagacac cgtcccctcc
     9121 acgcgagtga ccgcgtagac gccttcgata tcgtaactga ccttcccgcc gtgcatcgac
     9181 acattcacca ccttgctgga cgggttgggc tgaaacccgt tcaaggcgta ataattaaag
     9241 acggcatccg gattggcgag atgctcctcc ctcacacagc gagtgcgggt gtccggtttc
     9301 cctgcctcgc cgttgctggc atccgtcacc gccgtcactt gatattcgcc tggcttcatc
     9361 ggaaccgact ccgcccgcac cggcgcaaca ccgagggtga cgccgacaag cccatacagc
     9421 aacacgcgtg tcttcatctt caagcctcct catcttcccg gggtgcgccg gcccctgggt
     9481 ccgctgttcc ccccagaccg cctcgcccga agttctgccc catcaaggcc ttagcgattg
     9541 tgctcatgct ccgcactatt gtcaacggtg acggccggcg aacgatccgg acttgcacga
     9601 gcatacccga tctctcacct ggctcaattt ggtctccact atggtatagt cgcaacacga
     9661 ttctgttcgc ctcccgaaag gagtctacgc atgattgcca atcatcgccc gatcgtcgcc
     9721 gcattcggag gggtctctct cgtcttgctg ctctcggcct gctccttcaa agccaccttc
     9781 aaggaaacga cggacacgac ctcgaacatt accgggacca catccggacg catctggtgg
     9841 aacgaagacg gcctgctgaa acccgaacac aaggttgcgg ctttcaccgc ctacaatgcg
     9901 gacaatcttc aatccgacgc ggcccgcgga cagggcgagt acctggcctc gctggaccgc
     9961 ctcatgggcg cctccgacgc ctcggtattt caaccgttcg cgcaagactc attcagccgc
    10021 tggagccgga ccggaagcac ctcgaccacc gacctggtga acgatct
//
LOCUS       DAORRQ010000101        10062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_301_length_10062_cov_2497.270511, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000101 DAORRQ010000000
VERSION     DAORRQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_301_length_10062_cov_2497.270511"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /gene="lptC"
                     /locus_tag="PK224_19155"
     CDS             complement(<1..510)
                     /gene="lptC"
                     /locus_tag="PK224_19155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRB17925.1"
                     /translation="MWERWIRRGLLALSVVLAAFLGYLLVTRSNPGSSSRSVAPVDTE
                     SADARIQDFTFTQTKGDLVQWKVQAEQARLFEKESRAILNNVQITMYGAQGKELTLSG
                     DEGTLDTQTKNFQLSNRTTPIVVETQSGYTIQTNHLVWTDARHEIQTPDHVTINGHGL
                     QVTGRGLLGK"
     gene            complement(699..1145)
                     /gene="rplQ"
                     /locus_tag="PK224_19160"
     CDS             complement(699..1145)
                     /gene="rplQ"
                     /locus_tag="PK224_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRB17926.1"
                     /translation="MRHKKKGRQLGRQTKHRWALFRSLVTSLLEHERIETTEAKAKEV
                     RGFTDRMITLGKEGGLPARRRALSFLRSKAVVAKLFSDVASRFRERPGGYTRIIRTRR
                     RIGDAAEMVAIELVTRPEKPKAVPSTDVAVAEKKDTTEKTSAQAES"
     gene            complement(1164..2165)
                     /locus_tag="PK224_19165"
     CDS             complement(1164..2165)
                     /locus_tag="PK224_19165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRB17927.1"
                     /translation="MIKAMKDFQIPMRVEVDKDTLSPTFGRFTTEAFERGFGTTVGNS
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTSIILNVKSLRLALQGDKPKTI
                     RLKKKGPGEAKGSDITHDGDVTILTPDLHIATLDKDASLDLEMTIKHGRGFVPAERNK
                     EEGLPIGVIAVDSIFSPIKRVNFHVENARVGRMTDYDKLTLEIWTDGTISPRDALSNA
                     AGILREHLDIFINPEERSDAKPAVGSEDLSDEVNKNLYRSVNELELSVRAANCLKNAN
                     IKTIADLVQKTEAEMLKTKNFGKKSLNEIKEILTEMGLSLGMKVDALPSGDAGVRSE"
     gene            complement(2249..2875)
                     /gene="rpsD"
                     /locus_tag="PK224_19170"
     CDS             complement(2249..2875)
                     /gene="rpsD"
                     /locus_tag="PK224_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRB17928.1"
                     /translation="MAKYSGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QARQRTSDYSLQLREKQKLKRIYGLQECQFRGIFERAERQAGVTGDTLLRLLECRLDN
                     VVYRLGFGASRKEARQLVNHGHLMINGRKVKAPGALVKAGDSVEVRQKSRELLPIQGA
                     LAAVDGRGIPEWLELDRAACKGTIRSLPTKEHIVLPVNEQMVVELYSR"
     gene            complement(2957..3340)
                     /gene="rpsK"
                     /locus_tag="PK224_19175"
     CDS             complement(2957..3340)
                     /gene="rpsK"
                     /locus_tag="PK224_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRB17929.1"
                     /translation="MSVKKGKKKERKIVQSGVAHVQASFNNTIVTITDMSGNTVVWAS
                     SGNQGFKGSRKSTPFAAQRAGEAAARKAMENGMRQVDVYVNGPGAGRESAIRSLQSAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(3382..3771)
                     /gene="rpsM"
                     /locus_tag="PK224_19180"
     CDS             complement(3382..3771)
                     /gene="rpsM"
                     /locus_tag="PK224_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRB17930.1"
                     /translation="MARIAGVDLPKDKRIDIGLTYVYGIGRVAAQAILKKAGVDGSIR
                     VKDVSEDKIVKIREVIERDYRVEGDLRKEVSMNIKRLVDTGTFRGLRHRKGLPVRGQR
                     TKTNARTRKGRRSGVGSKPQKPAGSRA"
     gene            complement(3905..4018)
                     /gene="rpmJ"
                     /locus_tag="PK224_19185"
     CDS             complement(3905..4018)
                     /gene="rpmJ"
                     /locus_tag="PK224_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRB17931.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRVLCANPRHKQRQG"
     gene            complement(4037..4255)
                     /gene="infA"
                     /locus_tag="PK224_19190"
     CDS             complement(4037..4255)
                     /gene="infA"
                     /locus_tag="PK224_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRB17932.1"
                     /translation="MPKEDVIEVTGTVAETLPNAMFRVELEHGHRILAHISGKMRMHF
                     IRILPGDKVTVQLSPYDLTRGRITYRFK"
     gene            complement(4277..5032)
                     /gene="map"
                     /locus_tag="PK224_19195"
     CDS             complement(4277..5032)
                     /gene="map"
                     /locus_tag="PK224_19195"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRB17933.1"
                     /translation="MIVFKTPDEVSLMAQASHVVAEVLELLKEKVAPGITTDDLDRMA
                     EEAIRVRGAIPAFKGYRNYPKTLCASVNEQVVHGIPSKRRLKEGDIIGLDLGAIVSGF
                     YGDSAVTVPVGVASSEALRLIQVTEESMYRGIAKAVVGNRLSDVSHAVQTHVEQAGFA
                     VVTEFVGHGIGRQLHEEPQVPNYGRPGQGPRLQVGMVLAIEPMVNMGSSAIRILEDRW
                     TAVTHDGRWSAHFEHTIAIQPNGPARILSQLSH"
     gene            complement(5029..5685)
                     /locus_tag="PK224_19200"
     CDS             complement(5029..5685)
                     /locus_tag="PK224_19200"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRB17934.1"
                     /translation="MRLVFLGAPGVGKGTQADRVTAQFGWPKISTGDLLREAVRNQTA
                     LGIEAKKSMDAGKLVPDNVVIGMVRAKVAEPICQNGFILDGFPRTVPQAEELSGILAE
                     RGVSLDRVVNFRVSREDVIRRLSGRRSCPKCQSVFHVDFATPKIEGQCDRCGASLVQR
                     SDDKPETIEARLKVYDEQTAPLIAYYEKKKVLSDLDGSGDMTHVYDRLSRVLAGLGAK
                     "
     gene            complement(5695..7008)
                     /gene="secY"
                     /locus_tag="PK224_19205"
     CDS             complement(5695..7008)
                     /gene="secY"
                     /locus_tag="PK224_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRB17935.1"
                     /translation="MFERLLTSFQNIFKIPELRTRVLFTLGMLIVYRVGAHIPTPGIN
                     GEALSEFLQKQGGALLGFLDIFSGGSLSRLTIFALGIMPYISASIILQLLTVVIPHLS
                     KLAKEGERGRKKIIQYTRFGTIVIALIQGFGIAVGLEQMNQGAFVLSPGWGFRFMTVI
                     TLCAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPAAVAQTFDLYKVGQLSFPLL
                     VVLTLIMFGVVAAIVFLESGRRKVPVQYAKRVIGRRVYGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWIKAIGSQLAPGSLLYTLMYVGLIVFFCFFYTAVVMNPV
                     DMADNLKKYGGFVPGIRPGQRTSDYIYSVLTKITFAGAIYLAIVCVIPEFLIYKLNMP
                     FYFGGTSLLIVIGVGLDTAQQIESHLLNRNYDGFLQKGKLRGRTT"
     gene            complement(7001..7450)
                     /gene="rplO"
                     /locus_tag="PK224_19210"
     CDS             complement(7001..7450)
                     /gene="rplO"
                     /locus_tag="PK224_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRB17936.1"
                     /translation="MKLHDLAPSRGAKHKRKRIGRGPGSGHGKTATKGHKGLKARSGG
                     GKRPGFEGGQMPLIRRVPKYGFTNQFRTEYTIINLKSLADLETTEAITPQLLMNEGLV
                     RRKGELIKILAQGELTKPLVVQAHKFSKSAEAKIQAAGGRAEVIPCV"
     gene            complement(7447..7656)
                     /gene="rpmD"
                     /locus_tag="PK224_19215"
     CDS             complement(7447..7656)
                     /gene="rpmD"
                     /locus_tag="PK224_19215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRB17937.1"
                     /translation="MATEKKSTIAQASLRITLKRSPIGTPHKHRLVLRGLGLRKLNAT
                     VLRPASDQVKGMIAKVGYLLEVSPQ"
     gene            complement(7656..8159)
                     /gene="rpsE"
                     /locus_tag="PK224_19220"
     CDS             complement(7656..8159)
                     /gene="rpsE"
                     /locus_tag="PK224_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRB17938.1"
                     /translation="MRVNPEELSLKDKVVFINRVAKVVKGGKRFNFCALVVVGDGQGY
                     VGVGKGKAAEVPVAISKAVEQAKKHLVRVPIKGGTIPHEVHGLFGAEHVLLKPAADGT
                     GIIAGGAVRAVVELAGAHNIIAKTLGRGNPFNAVRATLHGLQQLCDPQEMMRLRRSAG
                     SEAQVHV"
     gene            complement(8234..8599)
                     /gene="rplR"
                     /locus_tag="PK224_19225"
     CDS             complement(8234..8599)
                     /gene="rplR"
                     /locus_tag="PK224_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRB17939.1"
                     /translation="MNTQEKNRKLARRKQRVRKSVIGTSERPRLNVFRSRAHIYAQII
                     DDLRGHTVAAASTLDESLRKSVKSTGSIEGAKAVGKLIAERAKAAKVSMVVFDRGGRQ
                     YHGRVKALADASREGGLQF"
     gene            complement(8609..9145)
                     /gene="rplF"
                     /locus_tag="PK224_19230"
     CDS             complement(8609..9145)
                     /gene="rplF"
                     /locus_tag="PK224_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRB17940.1"
                     /translation="MSRIGRKPISVPAGVDIKVAGRVVSVKGPIGKLDWKLTDGLSVA
                     VNDGQLVVNRSGDARQIRAMHGLVRAELSNIIQGVTKGYEKALEITGVGYKAQLQGRE
                     MSFNVGYINPVTYTVPAGIDVKVDKQTLISIKGVDKRLVGQVAANIRSIKPPDVYKQK
                     GIRYAGEVLRKKAGKTGK"
     gene            complement(9202..9597)
                     /gene="rpsH"
                     /locus_tag="PK224_19235"
     CDS             complement(9202..9597)
                     /gene="rpsH"
                     /locus_tag="PK224_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRB17941.1"
                     /translation="MITDPIADLLVRLGNAARRRQDVVKVPVSKVKRQILNILGKEGF
                     ILGYGEFQEDGHPYFSVQLRYVEEARPMITGMRRISKPGRRVYVGRDDVPKVRNGIGV
                     AVISTSKGLMTDSDSRRSGLGGEVLCSVW"
     gene            complement(9643..9828)
                     /locus_tag="PK224_19240"
     CDS             complement(9643..9828)
                     /locus_tag="PK224_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRB17942.1"
                     /translation="MSRLALKNKAAAKSKFACRDYHRCGLCGRVRGFLRRFRMCRICF
                     RFLSLKGEIPGVRKSSW"
     gene            complement(9886..>10062)
                     /gene="rplE"
                     /locus_tag="PK224_19245"
     CDS             complement(9886..>10062)
                     /gene="rplE"
                     /locus_tag="PK224_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRB17943.1"
                     /translation="FDGRGNYTLGLKEQLIFPEIEYDSVASIHGMDITIVTTARTNDE
                     GKALLKHLGMPFRA"
BASE COUNT         2367 a         3025 c         2290 g         2380 t
ORIGIN      
        1 ttttcccagc aatcccctgc cggtgacttg caacccatgt ccgttaatcg tgacatggtc
       61 tggagtttga atttcatgcc gcgcatcagt ccacacaaga tgatttgtct gaatggtata
      121 gccactttga gtttcaacca caatcggcgt ggtcctgttg gaaagttgaa agttcttggt
      181 ctgtgtatca agcgtgccct catccccgga caaggtcagt tcctttccct gagccccgta
      241 catggtaatt tggacattat tcagtattgc ccggctctcc ttttcaaaca gacgcgcctg
      301 ttcggcttga actttccact gaacaagatc gccttttgtc tgggtgaagg taaagtcttg
      361 aattctggcg tcggccgatt cggtgtcaac cggcgcgacc gaacgcgacg aagatccagg
      421 attggagcgt gtgacgagca aatacccaag gaaggcagca agcaccacac tcagagctag
      481 aaggcctcgt cgaatccaac gttcccacat ggcaaaaagc ccctaattta aagagcttcc
      541 caggtcacag tcggtggcat gatagtagca ttccaccctg gccaagtaaa cctacagccg
      601 gcgcatggcc tgagaaaatg atcggagata acgatgcaga gtgaggtggg gagagaacaa
      661 ggccacgacg cccctgaggt cgtcgtggct ctcgatgttt agctttctgc ttgggctgaa
      721 gtcttttctg tcgtatcttt cttctcagcg accgcgacgt ccgtcgatgg cacggccttc
      781 ggcttctctg gcctggtcac caattcaatg gccaccatct ccgcggcatc accgatccgc
      841 cgacgggtac ggatgatacg cgtgtagcct ccggggcgct cacgaaagcg cgatgcaaca
      901 tcgctgaaca gcttcgccac caccgccttg cttcgcaaga agctcagagc gcggcgacgg
      961 gcaggaagcc ccccctcttt cccaagagta atcatgcgat cggtaaagcc acgaacttcc
     1021 ttggccttgg cttccgtcgt ttcgattcgc tcatgttcca gtaatgaagt caccagactc
     1081 cggaacagag cccatcggtg tttagtttga cgtccgagct gtcgaccctt ctttttgtgg
     1141 cgcacggtag tcctgttcct ttcttactct gaacgtacgc ccgcgtcgcc tgacggcaag
     1201 gcgtcgactt tcattccaag gctcagtccc atttcggtca ggatttcctt gatttcgttg
     1261 agggattttt tcccgaaatt cttggtcttc aacatttctg cttctgtttt ttgcaccaag
     1321 tcggcaatcg tcttgatgtt ggcattcttc aggcaattgg ctgcccgaac ggacaattcc
     1381 aactcattca cgctgcgata gagattctta ttcacttcat ccgacaaatc ttcactgccc
     1441 acagccggtt tcgcatcaga gcgctcttca ggattaatga agatatccaa atgttcgcgg
     1501 aggatgcctg ccgcgttgga caacgcgtca cgcggagaaa tcgtgccgtc cgtccaaatt
     1561 tcaagcgtca gcttgtcata gtcggtcata cgacccacac gcgcattctc gacatggaaa
     1621 ttcacccgct tgatcgggga gaaaatcgag tccacggcga tcaccccgat gggcaatcct
     1681 tcttctttat ttcgttcagc cggcacaaat ccacggccat gcttgatcgt catttccaaa
     1741 tctagcgagg catctttgtc cagagtcgca atatgcagat caggagtgag aatcgtgaca
     1801 tccccatcgt gcgtaatatc agagcccttc gcttccccag gccctttttt cttgagccgg
     1861 atggtctttg gcttgtcgcc ctgcaacgcc aaccgcaggc ttttcacatt caaaataatg
     1921 gatgtgacat cttctgtcac gccaggaatc gtggagaatt cgtgcaacac tccttcgatc
     1981 ttgacggtgg ttaccgcagc ccccgtcaaa gaagacagca acacacgacg cagggagttc
     2041 cccaccgtgg tgccaaatcc tcgctcaaat gcctcggtcg taaatctgcc gaatgtcggg
     2101 gaaagagtgt ccttgtcgac ttccacccgc atggggatct gaaagtcttt catcgcttta
     2161 atcatgactc ccccttcatt aactcgtgtg tgcccaacta tcgttcagcc atgactggat
     2221 gagcaagccg ccatccagcc acgtattctt agcgcgagta caattccacg accatctgtt
     2281 cgttgaccgg gagcacgatg tgctccttgg tcggcaacga acgaatggtg cccttgcagg
     2341 ctgctcggtc caattcaagc cactccggaa taccccggcc atccacggcc gccagtgctc
     2401 cttgaatggg gaggagttca cggctcttct gccggacttc cacggaatcc cccgctttga
     2461 caagggcacc gggcgccttc acttttcgac cgttgatcat caagtgacca tgattgacca
     2521 actgccgggc ctctttccga gaggctccaa agccgagccg atacaccacg ttatccaagc
     2581 ggcattccag gaggcgcagc aatgtatcgc cagtgacgcc ggcttgccgt tccgcccgct
     2641 caaaaatgcc acggaattgg cactcttgaa gtccgtaaat cctcttgagc ttctgctttt
     2701 cacgcaactg cagactataa tccgacgtcc gctgacgagc ctgaccatgc tgcccagggg
     2761 gatagctccg gcgctcaatc gcgcacttct cggtcatgca acgagaccct tttaaaaaga
     2821 gcttctcgcc ctctctccga cacaagcggc agacaggtcc actatatttt gccacgctac
     2881 tacccctcct ctacctcaac gactcaagta cgactaaatc agcgattacc ttgttctgat
     2941 gacgtcggga ccacaactag acacgtcggc gcttcggagg ccggcatccg ttgtggggga
     3001 ttggcgtcac gtcgcgaatc agattgatgc gcaacccggc gctttgcaat gaacgaatgg
     3061 cggactcgcg gccggccccc ggcccgttga catacacatc aacctgccgc atgccgttct
     3121 ccatggcctt tctcgccgcc gcctctcccg ctcgctgcgc cgcaaacggt gtgctcttac
     3181 gggagccttt aaacccttga ttcccagaac tggcccacac cacggtgttc ccgctcatat
     3241 ccgtaatagt cacaatcgtg ttattgaacg acgcttgaac gtgagcgact ccgctctgaa
     3301 caatcttccg ttccttcttc ttgcccttct tcacactcat agaatctccc tcagcgcatg
     3361 gcgcctgctc ggtctaatct cttatgctct tgaaccagcg ggcttctgcg gtttgcttcc
     3421 tacaccggag cggcgcccct tgcgggtacg cgcattggtc ttggtccgct gtccacgaac
     3481 aggcaacccc ttgcgatgcc ggagcccacg aaatgttccg gtatccacca gtcgcttgat
     3541 gttcatcgac acttctttac gcaaatcgcc ttccacgcga tagtcacgtt cgatgacttc
     3601 tcgaattttg acgattttgt cttcactgac atccttcacg cgaatggatc cgtcgactcc
     3661 ggcctttttc aaaatcgcct gagcggcaac acggccaatc ccgtacacat aggtcagacc
     3721 gatatcgatc cgtttgtctt ttggcaaatc cacgcccgca atacgcgcca taattacccc
     3781 ctaattcctt cccttgattg caccgtgagc tggcttgaag gaccttcgca acaccgttcc
     3841 gatgtcgcca tgaccagcct cttgcaggct cgacataccc ctgccaaggc aacccacatt
     3901 ccggtcaacc ctgtcgctgc ttgtgacgcg gattagcgca taacacccga acgacaccac
     3961 gccggcggac cactttacat ttcgcacaaa tcggcttgac tgacgacttg accttcatag
     4021 caattccatt tctccactac ttgaagcgat aggtaatccg gcccctggtt agatcatagg
     4081 gcgacaattg cacagtcacc ttatcgccag gaagaatacg gatgaagtgc atccgcattt
     4141 ttccagagat gtgcgctaaa attctgtgcc catgttccag ttctacgcga aacattgcat
     4201 tcgggagtgt ttctgccacc gtgccggtca cttctatgac atcttctttt ggcacgtatc
     4261 agtacgcctc ccagtcttaa tgtgataact gtgacagaat tcgcgctggg ccattcggct
     4321 gaatggcaat cgtgtgctcg aaatgagcgg accaacgccc gtcatgagtc acagccgtcc
     4381 atcgatcttc taaaatacga atcgcactgc tgcccatatt caccatgggt tcaatcgcca
     4441 acaccatccc aacttgaaga cgcggcccct ggcctggccg tccataattt ggaacctgag
     4501 gttcttcatg gagctgccgt cctataccat gaccgacgaa ttcagtcacc acggcaaagc
     4561 cagcctgctc aacatgagtt tgcaccgcgt gtgacacatc agaaagccga ttaccaacca
     4621 ccgctttcgc gatgccacgg tacatcgatt cttcggtcac ttgaatcagc cgcagagcct
     4681 cgctgcttgc tacaccaacc ggtaccgtca cagcggaatc accgtagaag cctgaaacga
     4741 tcgctcccaa atcaaggccg ataatatcac cttccttgag gcgacgcttg gatggaattc
     4801 cgtgaacaac ctgctcgttg accgatgcac acagcgtctt ggggtagttc cgatatcctt
     4861 tgaaggcggg aatggcacca cgaactcgaa tcgcctcttc agccatacga tccaaatcat
     4921 cagtcgtaat gcccggtgcc accttctcct ttaacaactc caacacttct gccaccacat
     4981 gagaagcctg cgccattaac gagacttcgt ccggcgtctt gaacacgatc atttagcgcc
     5041 aagcccagcg agcaccctcg ataacctatc gtaaacgtga gtcatatcgc cagacccatc
     5101 aaggtcactc aggacttttt tcttttcata gtaggcaatt aacggcgcag tctgctcatc
     5161 ataaactttc aaacgggctt caatggtctc cggcttgtca tcgcttcgtt gaaccagcga
     5221 agcgccacag cggtcacatt gaccttcaat tttcggcgta gcaaaatcta catgaaacac
     5281 gctctggcac tttggacaac ttcggcgacc actcaggcgt ctaatcacat cttcgcgaga
     5341 gacccgaaaa ttaacgacgc gatcaaggct tacccctcgc tcggccagaa ttccactcaa
     5401 ctcttcggcc tggggaacag tccgtgggaa tccatctaag atgaaaccat tctgacaaat
     5461 cggttcagcc actttggcac gaaccatacc gataacgaca ttgtcaggaa caagtttccc
     5521 cgcatccata ctcttttttg cctcgatccc taaagcggtc tggttcctca cggcctctct
     5581 caagaggtct ccggtagaaa tcttgggcca accaaactga gcggtcaccc gatcagcctg
     5641 agttcctttt cccacccctg gtgcaccgag aaacacgaga cgcattgaat ttccttacgt
     5701 cgtccggccc cgaagtttac ccttctgaag aaatccatcg taattgcgat tcagtagatg
     5761 cgactcgatt tgctgagccg tatcaagccc gaccccgatc acgatcagta gagaggtacc
     5821 cccgaaatag aaaggcatat tcagtttgta gataagaaac tccggaatca cgcagacgat
     5881 ggcgagataa atcgcgccgg caaaagtaat tttcgtcaat accgaataaa tatagtcgga
     5941 ggtacgttgc cccggcctga ttccaggaac gaacccgccg tattttttca aattatctgc
     6001 catatcaacg gggttcatca ccaccgcagt ataaaagaag cagaagaaga cgatcaggcc
     6061 gacatacatc aaggtataca gcagcgatcc tggggctaac tgtgagccaa tagccttaat
     6121 ccacggcgtt tcaaaaaacc cggcaatggt ggcgggaaag gcaataatcg acgaagcgaa
     6181 aatgggagga atgactcctg ccgtgttgat cttgagggga atatgtgtac tttgcccacc
     6241 gtatacccgt cgaccaataa ctctttttgc atattgaacc ggcacttttc ttcgcccgct
     6301 ctccaaaaag acgatggcag ctaccacccc aaacatgatg agcgtcaata cgaccaagag
     6361 cggaaaactc agttggccga ccttatacag atcgaacgtt tgagcaacgg cggcagggag
     6421 cctggccaca attcccgcaa aaataatgag cgaaataccg ttcccgatac cacgctctgt
     6481 aatttgctcg cccagccaca tcagaaagcc ggttccggcg cagagcgtaa taacggtcat
     6541 gaatcggaaa ccccagcctg ggctgagcac gaacgctcct tggttcattt gctcaaggcc
     6601 gacggcaatg ccgaatcctt gaatcaaagc gatgacgatc gtaccgaatc gtgtgtattg
     6661 aataattttc ttccggcctc gctcgccctc tttcgctaat tttgagaggt gaggaatgac
     6721 caccgtcaac agctgaagaa taatcgatgc gctgatgtag ggcatgatcc ccagcgcaaa
     6781 gattgtcagt cgtgagaggg agccaccgga aaaaatatcc aggaagccta gaagcgcacc
     6841 accttgcttc tgcaggaact cagaaagagc ctctccattg ataccaggcg tgggaatgtg
     6901 agcgccgaca cgatagacga taagcatccc cagcgtaaac aacacgcggg tacggagctc
     6961 agggatcttg aagatgtttt gaaaactagt cagcagacgc tcaaacacag ggaatgacct
     7021 cggccctccc cccggctgcc tgaatctttg cctctgctga cttgctgaac ttatgagcct
     7081 gaaccactag aggcttcgtg agctctccct gggcaaggat cttaatcagc tctccctttc
     7141 gtcgcacaag gccttcattc atcaagagtt ggggcgtaat ggcttctgtc gtctccagat
     7201 cagccagact tttcaaatta atgatcgtat actcagtgcg aaactgattc gtgaagccat
     7261 acttcgggac ccgacgaatc aagggcattt ggccaccctc aaacccaggg cgcttccccc
     7321 cgcctgagcg agccttaaga cccttatgac ccttagtcgc ggtctttcca tgaccggacc
     7381 ctggaccgcg cccgattcgc ttccgcttgt gtttcgctcc tcgggaagga gcaagatcat
     7441 gcagcttcat tggggactca cttccagtaa atagcccacc tttgcaatca tgcccttaac
     7501 ctgatcgctg gcaggacgca acacagtggc attgagtttc cgaagcccca atcccctcag
     7561 caccaatcga tgcttatgcg gtgtcccgat agggctccgc ttcagggtga tacgaaggct
     7621 cgcctgagca attgttgatt tcttttcagt agccattaga catgtacctg cgcttcgctt
     7681 ccagcactgc ggcgtagcct catcatttct tgaggatcac acaactgctg aagcccgtga
     7741 agagtagctc tgacggcatt gaatgggttg ccgcgaccca atgttttagc aatgatgtta
     7801 tgcgctcctg cgagttcgac cacagcccgc acagcacctc cggcgatgat tccggttcca
     7861 tccgcagcag gcttaagcag cacatgctca gcaccaaaga gtccgtgtac ttcgtgcggg
     7921 atagtaccac ccttgattgg aactcgcacc agatgctttt tcgcttgctc aacagccttt
     7981 gaaattgcga cggggacctc tgccgcttta cccttcccta caccaacgta cccctgccca
     8041 tctcctacca caaccaaagc acagaagtta aaacgctttc cgcccttgac tactttggcg
     8101 acgcggttga taaataccac cttgtccttg aggctcaatt cttcgggatt gactcgcaca
     8161 acgtcattct cctttaagtc gtctcatttg gctcgtcacg agcagaagat ggttaatctg
     8221 cacgattcct tgcctaaaac tgtaggccgc cttcgcgcga cgcatcagcc aaggccttca
     8281 ctcgaccatg atactgccgc cctccgcgat caaagaccac cattgagacc ttggcagcct
     8341 tagcacgctc ggcgataagc tttccaaccg ccttcgcacc ctcaatactt ccggttgact
     8401 tgacggattt ccgcaacgac tcgtccaacg tggaagccgc agcaaccgta tgcccgcgaa
     8461 ggtcatcgat aatttgggcg taaatgtgag cacgactgcg aaacacattt aagcgtggcc
     8521 gctcacttgt tccgatgact gacttccgaa cacgttgttt acgacgtgct aattttctat
     8581 ttttttcttg ggtattcatc agatgcctct acttgccggt cttgcccgct ttcttcctca
     8641 agacttcccc ggcgtatcga attccttttt gcttatacac atcaggtggc ttaatcgagc
     8701 gaatgttcgc tgcgacctga ccgactagcc gcttatcaac gcccttaatg gagatgagag
     8761 tttgcttgtc gaccttgaca tcaatgcccg cgggaacagt atacgtcacg ggattaatgt
     8821 acccgacatt gaaactcatc tcgcgtccct gaagctgtgc cttgtaaccg acaccagtaa
     8881 tctcaagcgc tttctcataa cccttcgtga cgccctggat gatattactg agctccgcac
     8941 gaaccaaccc gtgcattgcg cgtatctggc gcgcatcacc agaacggttg acaaccagct
     9001 gcccgtcatt tacagcaacg ctcaatccat cggtcagctt ccaatcaagt ttcccaatcg
     9061 ggcccttgac tgacacaaca cgaccggcaa ccttaatgtc cacaccagct ggaactgaaa
     9121 ttggtttccg ccctatccgc gacatacgaa ccccgttacg ttacagcgac cccagttatt
     9181 accgcgcctc agccgacact tttaccagac ggaacaaaga acttcaccac ctaatccgga
     9241 ccgcctggaa tcactgtcgg tcataagacc tttcgaggtg gagatgaccg ccacaccaat
     9301 cccattcctt accttaggca cgtcatcccg accgacatac acccgacgtc caggcttgct
     9361 gattcgacgc ataccggtga tcatcggtcg cgcctcttca acatagcgaa gctggacgga
     9421 gaagtaggga tgaccatctt cttgaaattc gccgtaacca agaatgaatc cctccttgcc
     9481 aaggatgttc agaatctgcc gctttacctt ggaaactggg accttcacca catcttgacg
     9541 ccgacgagca gcgttcccta atctcactaa cagatcagca attggatcag taatcatgca
     9601 ttcctcttct acgcttgcag cggcaagctt ccagcaggcc gactaccagc ttgactttcg
     9661 cactccggga atttcaccct tgaggctcag aaatcgaaaa caaatacggc acatccgaaa
     9721 acgtcgcaaa aatccacgga cgcgaccaca gagcccgcat cggtgatagt cacgacaggc
     9781 gaactttgac tttgcggcgg ccttattctt gagcgctaat ctagacactg aatccccctt
     9841 actcgtactt gcctacaacg ccacacctac cgaactaaat gtgcactagg cccgaaacgg
     9901 cattcccaag tgcttgagca acgccttgcc ctcgtcattc gttctcgcgg tagtgacaat
     9961 ggtgatatcc ataccgtgga tggaggcgac actatcgtac tcaatctctg ggaaaatcaa
    10021 ttgctctttt aagccaagcg tatagttgcc acggccatca aa
//
LOCUS       DAORRQ010000102         9582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_309_length_9582_cov_28.044505, whole genome shotgun sequence.
ACCESSION   DAORRQ010000102 DAORRQ010000000
VERSION     DAORRQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9582
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_309_length_9582_cov_28.044505"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PK224_19250"
     CDS             complement(<1..219)
                     /locus_tag="PK224_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17944.1"
                     /translation="MRIPVATMGLTLTVGMLLVMQGGCSKKSIQSGGDAQSTERGMAK
                     SGGPAPAPLASTGGMDAPSATFPDLSLSS"
     gene            complement(289..1620)
                     /gene="tolB"
                     /locus_tag="PK224_19255"
     CDS             complement(289..1620)
                     /gene="tolB"
                     /locus_tag="PK224_19255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRB17945.1"
                     /translation="MNRVIIGLMIALCVVVGAGLFGILDSRATDVFLEATRPDFQKIP
                     IGVFGFQNGGGPEWLGGRIEEVLKADLQRSLVFSLVDLPAIGVKAREVSTTDKAIFKQ
                     AAENGVSVLVWGKSGQKNGSKDSELLMDGFVYDSGSDEVVGGKRYVGSTSVVRLMAHR
                     FADELVFRYTGEPGIARTKIVYVAEHGNARELFVMDYDGYEPKQITADGFLNLMPRWS
                     PDRRFIVFTAYRSRNTQDIDILELATGKRWTLVSMAGLNITPALSPDGNFLAFASSQD
                     GNSEIYKLDTRTKTPQRLTVNQGGDLSPTWSPTGREIAFTSDRGGAPQVFIMSADGSN
                     VRRLTYEGDYNAAPAWSPRGNWIAYVCRTAQRLYKICTVSPDGQKRVQVTTGPGIDDS
                     PSWSPDGRHITFSSTVDGKSHIYMVDTDGKGLERITFGGTHNSSPSWSPAL"
     gene            complement(1617..2837)
                     /locus_tag="PK224_19260"
     CDS             complement(1617..2837)
                     /locus_tag="PK224_19260"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRB17946.1"
                     /translation="MTFQALPRHTALFLIGEMGEAGSRRLRKTVVVSLVLHLCLLSVI
                     MGAKFFKKTERPLSAVEVSLVTLPPVEAKPEPKVEKAVPHPTPKPVQSAPMPVPPKPV
                     QTPTPPPIPPPVQAAPVAKPAPPAPAPAPAPRLQAPVLAAPQPVPQASKSPSSAPVTN
                     RADVLRDVMKDIELPPNAPQYGDLAPMKPAEVKKSAQPKVGGPERSDLDAMLKKLNVP
                     EMAAAQTAPPQEPPKPAVTPTKRASLSEEMNNDLDRELQDLKKLPSLPPVKATEPVRE
                     VKPMFREPQPVASAPPVTASVPRSKPETKLRVTGMSVGNPYWNIVKSRIVSFWAAPPV
                     DIKALTVVVRFRLERDGSIGGVTIEQTSGNAYFDMAAQRAVQSAVPLPRVPSELTDSY
                     FDTYFTFSAGEAAG"
     gene            complement(2879..3331)
                     /locus_tag="PK224_19265"
     CDS             complement(2879..3331)
                     /locus_tag="PK224_19265"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRB17947.1"
                     /translation="MTSETRHRRFMAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDIN
                     LPKSASNTIKPEARAVLSIERDQRLYLDKDAVSVVQLERKLRALKDQSPDVSLYLRAD
                     RDVPYGIVVQVMDSVKKAGIEKLGMVTEPTGAERVSESVATPAQPRKR"
     gene            complement(3328..4050)
                     /locus_tag="PK224_19270"
     CDS             complement(3328..4050)
                     /locus_tag="PK224_19270"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRB17948.1"
                     /translation="MFQSGVMGLVGSLGAVSKIVLLLLFVASILSWGVIFYKWRTFKA
                     ADREDQRFFSAFTKIRDLDELYRQSKRADGSPSARVFQGIMDRVLTVSGDGTVSASQP
                     ASASKDPVAAIDHQYMDKTVAYLVQNQVSHLESYLPVLATTGNITPFIGLLGTVLGII
                     DSFREIGTQGTASIAAVAPGVSEALVATAAGLFTAIPAVIFYNYYLTRIRKTVFRIES
                     FTVEAMRSLQTRMKQATVGGQL"
     gene            complement(4124..4201)
                     /locus_tag="PK224_19275"
     tRNA            complement(4124..4201)
                     /locus_tag="PK224_19275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4164..4166),aa:Asp,seq:gtc)
     gene            complement(4336..4411)
                     /locus_tag="PK224_19280"
     tRNA            complement(4336..4411)
                     /locus_tag="PK224_19280"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4376..4378),aa:Val,seq:tac)
     gene            complement(4556..5506)
                     /gene="xerD"
                     /locus_tag="PK224_19285"
     CDS             complement(4556..5506)
                     /gene="xerD"
                     /locus_tag="PK224_19285"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRB17949.1"
                     /translation="MSRESEDLSAPVPERLPLEPFIERYWDHVRIARGLSRNTQLAYQ
                     RDVATFQRYLRDRQVSDVREMSPPLLAGFLDHLHRSGLASSSRARAFAAVRSLFRFLK
                     QEGMIAANPTVSLRSTSRARRLPKTLSPEEVTRLLDLPPRPLPEDHRDRAMVEVLYAA
                     GVRVSELISLRVDQCNLDVGYVGITGKGDKQRVVPIGRPAIEALQAYLLAARPALLKQ
                     RSSRFVFVSRRGTPLTRQAFWKLLRARAHRAGIARIPSPHMLRHSFATHLLQRGADLR
                     SVQAMLGHADIATTQIYTHVDSSQLKKVHTACFPRNRSRR"
     gene            5670..7664
                     /locus_tag="PK224_19290"
     CDS             5670..7664
                     /locus_tag="PK224_19290"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRB17950.1"
                     /translation="MVPRSVPRAPHLRACSVALAVAFLAGLVTLGEAAPAKKNPAPSR
                     TEPSKPPTAAGQSVLDQAKRLIDSEQPEAAAVMLRRFIESGPVPDLLDDAYLLMAAAM
                     FGMKEHAETVRYVNQLLGEFPSSDLADRAKLLLAKTHARAGNLDLALPLLSEVRSLSA
                     DPAIKRDALRLTGEFQAQKKDYLRAIQAWLDEIPLDAGDQAHETEGQIRQLVNEQLDA
                     PALVRVREAYPKSFPGDLASIKLIELHTAAGEDHLVERDLRLFLSRFPNHPYAAKAAD
                     LQAVVRTKFKSHPYSIAAIFPMSGKLAPFGAEVLNGIQLALERSKDGGETPSIGLIVK
                     DTESDRAAFLDELSGVLSDDRPLAVIGPLLSKNLPVMAEMAERTRIPLITPSATAPNL
                     RRFGSYVFSTALTYGHQAKRVADYAIREQQYKRVAILYPDTAYGRDLARLFAQEVRQQ
                     DGELIVSEPYKEGDSDFRAVIGKLKAEDLKKYGVEVQVDNDPAKTGIRQGGKKGKRLL
                     YSPGFDAVFIPGRSLDVGLLAAQLAFFDIAVPLLGANGWNTPDFARVADRTVEGGVFA
                     DGFFAESSSPVVQEFVERYRKRFQATPSLFAAQGYDAARLAVEAIKRGATTGEGVRDF
                     LMMQHDLPTLSGPSGFSPDGTLNRRVFLIQVKQGKFVPLD"
     gene            7844..8833
                     /gene="trpS"
                     /locus_tag="PK224_19295"
     CDS             7844..8833
                     /gene="trpS"
                     /locus_tag="PK224_19295"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRB17951.1"
                     /translation="MTTGQKRVLSGMQPSGLLHLGNWLGALENWNALQEQYECFFFVA
                     DWHALSSNYADTSRIKEYVRELLIDWLAAGIDPKRATVFVQSQVPDHAILHLLFSMII
                     PVSWLERNPTYKEKQEEIKERDLSTYGFLGYPVLQAADILLYKPDFVPVGKDQLPHLE
                     LTRELARRFNSLYRPVFPDPQEHLTKFAKVLGTDGRKMSKSYGNAINLSDQEPVVRQK
                     IKTMITDPARVRRHDPGNPDVCPVYDFHKIFSPLPVIEQVNQDCRKAAIGCIDCKKLV
                     ADRVVERLAPMWDARATLTQHPSRLDEIAEDGRRRATAVSSQTMAEVRDAMKI"
     gene            complement(9066..9389)
                     /locus_tag="PK224_19300"
     CDS             complement(9066..9389)
                     /locus_tag="PK224_19300"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB17952.1"
                     /translation="MKNQRKHERVHVQFRSHFSMKGKMLAGDGDLTDLSPGGCRILSS
                     VQVLVGAEVELCIFPGDSANPILIDGATVRWCQPNEFGLSFTKIRVPVQRQLTDVWRK
                     LAKPA"
BASE COUNT         2071 a         3020 c         2707 g         1784 t
ORIGIN      
        1 gctggagaga gacaagtccg ggaatgtcgc actgggagca tccatccccc cggttgacgc
       61 gagtggagcc ggagcgggcc cgcccgactt ggccatacca cgctccgttg actgcgcgtc
      121 accacccgac tgaatagact tcttcgagca cccaccctgc atgaccaaca gcatcccgac
      181 tgtcagtgtc aggcccattg tcgctaccgg tatcctcata tcgtgactcc tcatggattg
      241 gtgaatggtt aatatatcgt agactcgggt attgcctgat gatcgtacct acaacgccgg
      301 ggaccaggac ggcgaactat tgtgcgtgcc accgaacgtg atgcgttcca accctttccc
      361 gtccgtatcc accatataga tgtggctttt gccgtcgacg gtcgaactga acgtgatatg
      421 ccggccgtcg ggagaccatg acggcgagtc atcgatcccg ggccccgtcg tgacctgaac
      481 ccgcttctgt ccgtcggggg acacggtaca gatcttatac aaccgctgag cggtccggca
      541 gacatacgca atccagttgc cgcgcggcga ccaggccgga gcggcattgt agtcgccttc
      601 gtaggtcagg cgacgaacgt tggacccgtc tgcgctcatg atgaagactt gaggagcgcc
      661 tccgcggtcc gaggtaaatg caatctcgcg gccggtgggc gaccaggtcg gcgagagatc
      721 gcccccctga ttcaccgtca gccgctgcgg agtcttggta cgggtgtcga gtttataaat
      781 ctcggaattg ccgtcctgac tgctggcaaa cgccagaaaa ttcccgtctg gcgacaaggc
      841 aggcgtaatg ttcagccccg ccattgagac cagcgtccac cgtttgccgg tcgccagttc
      901 caggatatcg atgtcctggg tattccggct ccgataggct gtgaacacaa tgaaccggcg
      961 atcaggcgac cagcgcggca tcagattcag aaagccgtcc gccgtaatct gcttgggctc
     1021 atatccatcg tagtccatca cgaacaattc acgggcattg ccatgctccg cgacatagac
     1081 aattttggtc ctggcgatgc ccggctctcc ggtataccga aacaccaatt catcggcaaa
     1141 ccggtgcgcc atcaaccgca cgaccgaggt cgaacccaca taacgctttc caccgacgac
     1201 ctcatcgctg ccgctgtcgt agacgaagcc gtccatcaac aactcgctgt ctttgctgcc
     1261 gttcttctgg cccgacttcc cccagaccag cacggagacg ccgttctctg ccgcctgctt
     1321 aaagatcgcc ttgtcggtgg tcgacacctc acgcgccttc accccgatgg caggcaaatc
     1381 caccagcgaa aagaccaagg accgttgaag atccgccttc agcacttcct cgatacggcc
     1441 cccgagccat tcgggaccgc cgccgttctg aaaaccgaat actccaatgg gaatcttttg
     1501 aaagtccggc cgggttgcct ccagaaacac atcggtcgcg cgggaatcca gaatcccgaa
     1561 gagaccggct cccaccacga cacagagggc aatcataaga ccgatgatca cacgattcat
     1621 cctgcagcct cgcctgctga gaaagtgaag tatgtatcaa aatatgaatc agtcagctcg
     1681 gaagggacgc gtggtagcgg taccgcactc tgcacagctc gctgtgctgc catatcaaag
     1741 taggcgttgc cagacgtctg ttcgattgtc acaccaccaa tacttccatc acgctccaaa
     1801 cggaaccgca ccactacggt gagagcctta atatcaaccg gcggggcggc ccaaaagctt
     1861 acaatcctcg actttacaat attccaatac gggttcccca cagacattcc agtcaccctt
     1921 aactttgttt caggcttgct gcgggggaca ctggccgtca ccggcggagc actcgcaacc
     1981 ggctgcggtt cgcggaacat cggcttcacc tcccgcaccg gctcagtcgc tttgaccggt
     2041 ggaagggatg gcagtttctt caaatcctgc agttcgcgat caaggtcgtt attcatctct
     2101 tcggaaagag aggcgcgctt cgtgggagtg acagccggct tgggaggttc ctgcggaggc
     2161 gcggtttggg cggcggccat ctcgggcacg ttcaatttct tcagcatcgc atccaggtct
     2221 gatcgctcag gtccacccac tttcggctga gcagactttt tcacctccgc cggtttcatc
     2281 ggggccagat cgccatactg gggcgcattc ggcggcaact cgatatcttt catcacatcc
     2341 cgaagcacat ccgcccgatt ggtcacgggg gcggaagaag gcgacttcga agcctgcgga
     2401 acgggctgtg gcgccgccag cacaggagcc tgcaaacgag gcgcaggagc cggtgccgga
     2461 gctggtggcg ccggcttggc caccggggcg gcctggaccg gaggcggaat cggaggcggt
     2521 gttggggtct gcaccggctt gggaggcacg ggcatcggag cagattgcac cggcttgggt
     2581 gtgggatggg gaaccgcctt ctcaaccttc ggctcgggct tcgcttccac gggcggcaac
     2641 gtgaccagcg agacttccac cgccgagagc ggacgttctg ttttcttaaa gaacttggca
     2701 cccataatca cggataggag gcagaggtgg agcaccaggg acaccacaac cgtcttgcga
     2761 agacggcggc ttccggcttc acccatctcc ccgatcagaa agagcgcggt atgtcttggc
     2821 agggcttgga acgtcatgac ttgcgatacg accgtgtggc gcgcgagtcg cggcgccgct
     2881 atcgtttgcg tggctgcgcg ggggtcgcga ccgactcact cacgcgttcg gcacctgtag
     2941 gctccgtcac cataccaagc ttctcaatcc cggctttctt cacactgtcc atcacctgca
     3001 ccacgatccc gtaaggcacg tcccgatcgg cgcggagata gagcgagaca tcggggcttt
     3061 gatctttcaa ggcccgaagt ttccgctcga gttgcacgac actcaccgcg tccttatcca
     3121 aatacaaacg ctgatcgcgc tcaatggaca aaaccgctct ggcttcaggc ttaatcgtat
     3181 tgctggcgga cttgggaaga ttgatatcca tcccgcgata caacatgggg gcggtcacca
     3241 tgaaaatcac gagcagcacc aacaccacgt ccaccagcgg gatcacgttg atctccgcca
     3301 tgaagcgccg gtgtctggtc tccgacgtca caactgcccc ccaactgtgg cctgcttcat
     3361 ccgggtctgc aatgaacgca tcgcctccac cgtgaacgat tcgatccgga acacggtctt
     3421 acgaatgcgg gtcagataat agttgtagaa gatcacggca ggaatagcgg taaacaatcc
     3481 ggccgcggtc gccaccaacg cttcggacac gccgggggcc acagccgcga tgctcgcagt
     3541 tccctgagtc ccgatctcac gaaacgagtc gatgataccc aataccgtcc ccagcaatcc
     3601 gatgaagggg gtaatgttcc ccgtcgtggc cagcacgggc aggtacgatt ccaaatgcga
     3661 gacttggttc tgcaccagat aggccacggt cttgtccata tattgatgat cgatcgcagc
     3721 caccggatcc ttcgaggcac tggccggttg agacgcactc actgtgccat ccccggacac
     3781 cgtcaggacg cgatccataa tgccctgaaa tacacgcgcg ctcgggctgc cgtcggcccg
     3841 cttggattgg cgatacagtt catcgagatc gcgaatcttc gtaaacgcgc tgaagaaccg
     3901 ctgatcctcg cgatccgccg ctttgaaagt ccgccatttg tagaaaatca cgccccagga
     3961 aagaatcgaa gcgacaaaga gcagcagaag aacgattttc gagaccgccc ccagtgagcc
     4021 caccaacccc attacgcctg attggaacat gagcctagac ctttcccttc acatggcgtc
     4081 gcatgaaaac ggtaagcatg gattgtacca aaagctgggg gaatggcggg gccgacggga
     4141 tttgaacccg cgacttccag attgacaatc tggcgtccta accaggctga acgacggccc
     4201 cacgcaaccc aagatggcgg aagaagaatt cagatcttca gaatcaaaca gtctaaatac
     4261 ggtagagtgt actcttgtac atcactacta atggataagg caatgcctta cgcgcaattc
     4321 cctgtatttc ttaattggta ggcggaacag ggatcgaacc tgtgacctct gccttgtaag
     4381 ggcagcgctc tcccaattga gctatccgcc cgatttttct cgttcccggc agatttttgg
     4441 gatgctatca actccacaaa gcagtgtcaa ccaggaatca tgcattattc ttgcaggatt
     4501 cgtggaaagc aaaaatttct atcacggtgg tactcacgga cggcacgaag ggggctcaac
     4561 gacgcgatct gttgcgcggg aaacacgcag tgtggacttt tttcaactgt gacgaatcca
     4621 catgcgtgta aatttgcgtc gtcgcgatgt cggcatgccc caacatcgcc tgcacggatc
     4681 gcaagtcggc cccccgttgc aacaagtgcg tggcgaagga atgtcgcagc atatgaggcg
     4741 aaggaatcct ggcgatgccg gcccggtgcg cacgcgcgcg gagcaatttc cagaaagcct
     4801 gcctcgtgag cggcgtgccg cgacggctca cgaacacaaa tcgtgacgac cgctgtttca
     4861 gcagcgccgg tcgcgcagcg aggagatagg cctgcaacgc ctcaatcgcc ggacgtccga
     4921 tcggcacgac acgctgttta tcgcccttgc cggtgatacc gacatagccg acatccagat
     4981 tgcactggtc aacccgcagc gagatcaatt cggacacacg caccccggcg gcatagagca
     5041 cctccaccat cgcgcgatca cgatgatctt ccggcaatgg acggggcggc agatccagaa
     5101 gacgcgtcac ctcctcggga ctgagcgttt tcggcaaccg tcgggcgcgc gaggtgctgc
     5161 gcaaactcac ggtcgggttg gcggcaatca tcccctcctg cttcagaaag cggaacagac
     5221 tcctgaccgc ggcaaacgcg cgcgcgcggg acgacgacgc gaggcctgac cgatgcagat
     5281 gatcgagaaa tccggccagc agcggaggcg acatctcgcg cacatcagag acctgccgat
     5341 cccggagata gcgctgaaac gtcgcgacgt cccgctgata ggccagctgc gtattgcgtg
     5401 acaagccccg cgcgatccgc acatgatccc agtagcgctc gatgaacggc tccagcggga
     5461 gtcgctccgg gaccggcgca gacagatcct ccgactcacg gctcatggga aacacccttg
     5521 ctgaatgcga cgaggaacag gctgcatcga ctataaccaa gcctccttcc gaacacaacg
     5581 catcgacctc gagttacctt gacacccccg cacctgtttc ctatagtagc ctcacttgcg
     5641 gcgagagcgt tcgccatgca caggagtcaa tggttcctcg atcagtaccc cgcgccccgc
     5701 atctccgcgc gtgcagcgtc gccctcgcgg tggcgttcct cgcgggcctc gtcacgctcg
     5761 gtgaggcagc ccctgccaaa aagaatccag cgccgtcccg cacagagcca tcgaaaccac
     5821 cgactgccgc cggccagtcc gtgctcgacc aagccaagcg cctgatcgac tccgagcaac
     5881 cggaagccgc agcggtcatg ctgcgacgct tcatcgaaag cggccctgtg ccggacctcc
     5941 tcgacgacgc ctatttgctg atggctgccg ccatgttcgg catgaaggaa catgcggaaa
     6001 ccgtccgcta cgtcaatcag ctgctcggcg aatttcccag ctctgacctg gccgatcggg
     6061 ccaaactgct gctcgccaaa actcacgcgc gcgcgggcaa cctcgacctg gcgctcccgc
     6121 tgctctccga ggtccgcagc ctctccgccg atcccgccat caagcgcgat gcgctgcggc
     6181 tcacgggcga attccaggcg cagaagaaag actacctccg ggccattcaa gcctggctgg
     6241 atgagattcc gctggatgcc ggcgaccagg cccatgaaac cgaaggccag atccggcaac
     6301 tcgtcaatga gcaactcgac gccccggcgc tcgtgcgcgt ccgcgaggcc tacccgaaat
     6361 catttccggg agatctggcc tccatcaagc tcatcgagct ccataccgcc gccggcgaag
     6421 atcatctggt cgaacgggat ttgcgcctct tcctgagccg cttccccaac catccctatg
     6481 ccgcaaaagc ggccgacctc caggcggtgg tgcggacaaa gttcaaatca catccctact
     6541 ccatcgccgc catctttccc atgtccggaa agctggcccc tttcggagcc gaggtattga
     6601 acggcatcca gctggccctc gagcgctcca aagacggcgg cgagacaccc tcgatcggcc
     6661 tgatcgtcaa ggatacggaa tctgaccgtg ccgcatttct ggatgaactc tccggcgtat
     6721 tgtccgacga tcgcccactc gccgtgatcg gcccgctcct gtccaagaac ctgcccgtga
     6781 tggcagagat ggccgaacgg acacgcattc cgctgatcac gcccagcgcc accgccccga
     6841 acctgcgacg attcggcagc tacgtcttca gcacagcgct tacctatggc caccaggcca
     6901 aacgcgtggc ggactatgcg atccgggaac agcagtacaa acgcgtcgcc attctctatc
     6961 ccgataccgc ctacggccgc gacctggccc gcctgttcgc gcaagaagtc cggcaacagg
     7021 acggagaact gatcgtgagc gagccctaca aggaaggcga cagcgacttt cgcgccgtga
     7081 tcggcaagct caaagccgag gatctgaaga aatacggcgt ggaagtgcaa gtcgacaacg
     7141 atccggccaa aaccgggatc agacagggag gcaagaaagg caagcgcctg ctctactcac
     7201 cggggttcga cgccgtgttc atccccggac gttcgctgga tgtgggactg ctggcagccc
     7261 aattggcgtt ttttgacatc gcggttccgt tgcttggggc caacggatgg aacactccgg
     7321 atttcgcgcg ggtcgccgat cgaaccgtcg agggaggcgt ctttgccgac ggatttttcg
     7381 ccgaaagcag cagtccggtg gtacaggaat ttgtggaacg gtatcgcaaa cggtttcaag
     7441 cgaccccttc gctgttcgct gcccagggct atgacgccgc acgattggct gtggaagcca
     7501 tcaagcgggg ggcgaccacc ggcgaaggcg tccgcgactt cttaatgatg caacatgacc
     7561 tgccgacctt gagcggcccg agcgggttca gtcccgacgg cacactcaat cgccgcgtct
     7621 ttctgattca agtcaaacag ggaaagttcg tcccgctgga ctgacgggtg cggtgccggt
     7681 ggaaagaaaa tcgatccatt gaaatttcag gcgagatcga gcagcgactc aggatgctca
     7741 ccagggccgt ccagcaaggc cgcagcgagc gaagcaccga ggcaaagcga ggacgaggcc
     7801 ggcgggctct ttcagcatcc tgctagaaca caggagtgat ggcatgacga cgggtcagaa
     7861 acgggtactc agcgggatgc agccgagcgg cctgctccat cttggcaatt ggttgggcgc
     7921 gctggagaac tggaacgcgc tgcaggagca gtacgagtgc ttcttcttcg tggcggactg
     7981 gcatgcctta tcctccaact acgccgacac cagccgcatc aaagaatatg tgcgggaact
     8041 gctgatcgat tggctggcag ccgggatcga cccgaagcgc gccaccgttt tcgtccagtc
     8101 ccaggtgccg gaccatgcca ttctccatct gttgttttcc atgatcattc cggtctcctg
     8161 gctggaacgg aacccgacct acaaagagaa gcaggaagag atcaaggagc gcgacctgag
     8221 cacctacggc tttctgggct acccggtcct gcaggccgcg gacatcctcc tctacaagcc
     8281 cgatttcgtg ccggtcggca aagaccagtt gccgcacctc gaactcactc gcgaactcgc
     8341 gcgccgtttc aacagccttt atcgtccggt gtttcccgac ccgcaggaac acctcaccaa
     8401 gtttgcgaag gtcctgggca cggacgggcg caagatgagc aagagttacg gcaacgccat
     8461 caacctctcc gatcaggaac ccgtcgtccg gcagaagatc aaaaccatga tcaccgatcc
     8521 ggcgcgggtg cgacgccacg accccggcaa cccggatgtc tgccccgtct acgactttca
     8581 caagattttc tccccgctgc cggtgatcga gcaggtcaac caagactgtc gcaaggcggc
     8641 tatcggttgc atcgattgca agaagctggt ggccgatcgg gtggtggagc gcctggcacc
     8701 gatgtgggac gcccgcgcca cccttaccca gcatccgtcg cgcctggacg agatcgccga
     8761 agacgggcgc cgccgcgcca cggccgtctc atcccagacc atggccgaag tccgcgacgc
     8821 gatgaagatc tagtcgcgcc tatccaatcg gggcaattca cttccagaat aaaacgcaac
     8881 tagcagcgcg ttgatcaacc gtacgctgca tccagaaaag gatcgttgtg cgacgtgcgt
     8941 agttgcgtct ggcgccgtgc aggctgttca gcaaggccga agcaagtgaa gaggagaatc
     9001 tcgcgccgta cggtgagctt ctgaacgagg cgagaacgcc gctggcggcc tttatcaaca
     9061 gcttgctagg cgggtttggc gagtttgcgc cagacatcgg tcaactggcg ctgcaccggt
     9121 acacgaatct tggtgaacga cagcccgaat tcgttcggct gacaccagcg cacggtggcc
     9181 ccatcgatga gaatggggtt ggcactgtcc cccgggaaaa tgcacagctc cacctcggcg
     9241 ccgaccagaa cctgcaccga gctgagaata cgacagccgc cgggagacag gtcggtcaga
     9301 tccccgtctc cggccagcat cttccccttc atcgaaaaat gactgcgaaa ctgaacgtga
     9361 acacgttcat gcttgcgttg attcttcatt gtgaattcgg cctccgtgcc cgtcgacaga
     9421 cggtgcttcg catcactcgt acgataggcc agaaccccgt aaaacgccag cactgcccgt
     9481 ttcttggcag gaagggtcta gcgaagagca gggcgaactg cgcacatcgt gcaggcaggc
     9541 ttcgcgacgc tacagggctt ccggcttggg ttcccctatc ga
//
LOCUS       DAORRQ010000103         9536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_312_length_9536_cov_17.316528, whole genome shotgun sequence.
ACCESSION   DAORRQ010000103 DAORRQ010000000
VERSION     DAORRQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_312_length_9536_cov_17.316528"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3109)
                     /locus_tag="PK224_19305"
     CDS             complement(<1..3109)
                     /locus_tag="PK224_19305"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRB17953.1"
                     /translation="MTEPAQIPDQAAREAAATTFDRNVVVIAGAGTGKTTLLVNRLLY
                     LLMRRTDPLDLSRIVALTFTNKAATEMKLRLRERLRSLVNVEGRDNPAAGSGTVSIAD
                     LRMRYGWTTDEIVARAGAALRDVEKAQIGTLHSFAAHLLRLYPIEAGVTPTFQTDEDG
                     SRFEEHFTSEWELWLDRELGAAGSDHGRWRILLDTFSLDELRELAYSLHSDLIDLNGL
                     MQQVAETGLNPGLRDWFSIRKAQAERLLGGYDRPKRRKIELMLAAVSDLFALLLAEGL
                     GAVRSFPREPQELLAKDLGAAPTGWEEDDYEAVQSLQRIAKRLLRVDHELLQNLLALL
                     APFVQSVRKSFLDSGWLTFDGLVGKARTLLRDHPPIREQLKRDYRALLVDEFQDTDPV
                     QYEIVLYLAERPGTQAGSWRETELESGKLFIVGDPKQSIYAFRRADIEAFDHVVARLE
                     GSGALRCELATNFRSHAQVLDLVNGVFNRLLVAQPAIQPPNVPLTVQPNRSSQFRNPG
                     VELRLVVSEDDGDLDSAAATRVEAEQIALMVSGLLRPAGQGGEAGGPESGLRPGHIAL
                     LFRKLTQAEHYLEALRRHGIAYIIDGEKHFYRRQEVIDLVNMLRCVDNPHDRIALVGL
                     LRSAVGGLPDSALVGLQERQALDYREVERLTDWSSPHAEPLRRLYGRLVRLHEQAPRC
                     PLPEVLDLIFAQLPVLELAAASLHGEQAVANLFKVRQMAADLADRPNLTLNGFVSLML
                     DRLTEQPEEAESALSEDTLDAVRVLTIHKAKGLEFPLVILAGLHHGDGAGRGPARPLI
                     WHDWSTGVQGLDLGDRCSLGAVLVAEKARTREQAERRRLFYVGMTRARECLVLSGALP
                     RRRVRGALLELLEEAAGAELGLAEQQDIPVGAVGLRQIVLQGDDRPPARQRERPVTLE
                     GAVMDRAFSERWMQRDRNWQAQRSESLLVSPSNFIQKPAPASEREPGRVRRVTPGKTV
                     GTIVHRLLQYWDFTADVASQVALIDHTSLALDEPDTAMQEAVIEEVRDLLRTFAQSAA
                     YDRLRR"
     gene            complement(3102..6332)
                     /locus_tag="PK224_19310"
     CDS             complement(3102..6332)
                     /locus_tag="PK224_19310"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRB17954.1"
                     /translation="MLRLVTGPFHPTLESQLVHDLRVLKSGDPQAAVALVVPSDQLRR
                     SLKRLLVVKRGLALLNVHILSFHQLALQLLRERRTTAGAAGAVRQLELVTDTFFERLL
                     QHLGQRNVPQTGALRLPQLPSGAWPALWASVRDLKDATVDSALALRAVEEGQFPPEDG
                     EKLKGLFTLYAALRDGSAALGVGSPDDLAALVTDFVPASPFLRSLNGLWYYGSYDLTQ
                     TQLTLLESLSISLPVTVYFPLSERPAYGFARQFLERHLYPIAGGVDERAGAPSGDGAH
                     ASQEVNVSVEVRNAAGIDDELTLVCKQILSLVETNGFAFDEIGVVGRTLVPYQASLKR
                     TFDQHRIPFLSSATVPLLQEPSVKTLLHLARLKGSGLHRPAMLEVITSSWNRRLTANR
                     GGVEPRPDLWRLAVLALGITRGEEEWRRLAQLGRLESWSSDADDPVTEELESLSIDGV
                     QLRLLWGCLSPLIDDVKRLPEQGGYGTLTDAFCSLAETHLTVPLGVSTSIDATAQWDD
                     ATDVNEALSRVFAQLRALDRLDINLTWDEWTETFAEILARTSRALAPSSHHGVQVLDA
                     MAARGVGFRALFLVGMNEKLFPRYIHEDGFLRDRHRLVLSETLGYKIDQKLQGYGEEA
                     LLFELLRASAGERLYLSYQRADAAGRPLAPSGYLDAVGVVPHPSDPDSLFALPRRWPD
                     RVDLPLFTPHLQTREELAVSMVLQGRDVTALLEEVGRDGWLFSHGLGAQGVIESGHPA
                     LSAYDGMLEHAGAHWNTVTERGFSPTALETYARCPFQYFSGQVLKLESVRHVPSMELT
                     PPAMGQLCHDALRFCYLALIQQGWPATTLPASVIIAEVNQAVAQTFEAYALTHGTGYA
                     LTWELAQESVRRLIEATVTLDRKMALVSGFHPAEFELVANGLLPDGSGGEDIPLRGRW
                     DRVDRHPGSGAVRVIDYKYRANDRVEAKDRNLLQAALRAQRLQPALYALMTPAGSSGD
                     REKALPEQVEFLYLLPHADPAVERVSFAASAWQGPSGPMLSRTMQVLLDGIRAAQYFI
                     VPDAHCTHCEFSTACRRAHQPSWWRAYRSSQARTLRDVRSQKVARD"
     gene            complement(6439..6603)
                     /locus_tag="PK224_19315"
     CDS             complement(6439..6603)
                     /locus_tag="PK224_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17955.1"
                     /translation="MTVPDDDDFELPILPMIDKGPPPECGTCGDPMKFIDGDWACVDC
                     NGELLGPETG"
     gene            complement(6616..6882)
                     /locus_tag="PK224_19320"
     CDS             complement(6616..6882)
                     /locus_tag="PK224_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRB17956.1"
                     /translation="MPNITLLHSPTCGACPSAKRLWKELRVKYSFSYREVDITTPDGQ
                     ELANRHSVRAVPATIIDGRLTFVGVPPRQSAEKALQLKMKPQGA"
     gene            7039..8691
                     /gene="pgm"
                     /locus_tag="PK224_19325"
     CDS             7039..8691
                     /gene="pgm"
                     /locus_tag="PK224_19325"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HRB17957.1"
                     /translation="MALHPRAGQPAQPEQLVNLEKLEQAYYGEVPDPSDPRQQVSFGT
                     SGHRGSSLRCTFNEAHILAITQAICEYRAQAGTTGPLFIGKDTHALSAPAQRSALEVL
                     AANGIQVRMDQADGYTPTPVISHAILTTNQGRTSGLADGIVITPSHNPPEDGGFKYNP
                     PHGGPADTEVTKAIETRANALLATKLHGVKRVPYDQALKAASTTRHDFVGHYLADLAN
                     VVDLQRIKAAKLRLGVDPLGGAAVAYWRPLAERYGLDLHIVNESVDPTFRFMTLDWDG
                     KIRMDCSSPHAMASLIKLKDRFDLAFGNDTDTDRHGIVTPGSGLMNPNHYLAAAISYL
                     FTHRPGWNAGAGIGKTVVSSSMIDRVANDLQRPMVEVPVGFKWFVPGLRDGSLGFGGE
                     ESAGAAFLRRDGTTWVTDKDGIIMDLLAAEMLAVTGKDPAQLYADLTARLGEPVYERI
                     DAPATRAQKAALQKFSPQQVQSRELAGEPITAMLTAAPGNMASIGGLKVTTANGWFAA
                     RPSGTEDVYKLYAESFNGQAHLRRIQEDAQALIKQVFSSAGA"
     gene            8887..>9536
                     /locus_tag="PK224_19330"
     CDS             8887..>9536
                     /locus_tag="PK224_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRB17958.1"
                     /translation="MSRFSHTLCTLLVGAALSACAAPASQGLRQAVTPLPDCCRTTQA
                     DLRKQAIVQTAVNLVGAKTIESQGRRISYDCAGVTRAIYLAHGIDLFEGGAADGKANG
                     VGLIYNHLRKHGQLHRGPVVQAGDLVFFDNTWDYNGDGLVNDPLTHVGIVERVESNGT
                     IVFISRVAGAIERYRMNVAHPHIHRTADGRLLNDYMRRKHWRDGEQTPYLTGELFAA"
BASE COUNT         1980 a         3108 c         2751 g         1697 t
ORIGIN      
        1 cccgccgcag tcgatcgtag gccgcagatt gggcgaaggt tcgcaacagg tcccgtacct
       61 cctcgatcac agcctcctgc atcgccgtat ccggttcatc gagcgccagc gaagtgtggt
      121 cgatcaacgc tacctgtgac gccacatcgg cggtgaaatc ccagtattgc agaagccggt
      181 ggacgatggt ccccacggtc ttccccggcg tcacccgtct cacccgccct ggttccctct
      241 cggaagccgg ggccggtttc tgaatgaagt tcgacggcga gaccagcaag gattccgatc
      301 gctgcgcctg ccagttccgg tcgcgctgca tccaacgttc ggagaacgcc cggtccatga
      361 ctgcgccttc taatgtgacg ggccgttcgc gctgcctggc gggcggacga tcatcgccct
      421 gcagaacgat ctgacgcaat ccgacagcac cgaccggaat gtcctgctgt tcggccaggc
      481 cgagttccgc tccggccgcc tcctccaaca attccaacaa cgcccctcgc acccgccgcc
      541 gcggcagggc tccggacaac accagacact cccgtgcgcg cgtcatcccg acatagaaca
      601 gccgacgccg ttccgcctgt tcacgagtgc gcgccttctc ggccaccagc accgcaccca
      661 ggctacagcg atcccccaag tcgagaccct gcacgcccgt cgaccagtca tgccaaatga
      721 gcggtcgcgc aggaccacgc cccgcgccat ctccatggtg taacccggcc agaatcacca
      781 gcgggaactc caggcccttt gccttatgga tggtgagcac gcgcaccgca tccagcgtat
      841 cctccgagag cgcactctcc gcttcttcgg gttgctccgt gagccgatcc agcatgagcg
      901 agacaaagcc gttcaacgtg agattcggac gatccgccag atccgccgcc atctggcgaa
      961 ccttgaagag attggccacg gcctgttctc cgtgcaggga cgccgccgcg agttcgagta
     1021 ccgggagctg cgcaaaaatg aggtccagca cctcaggcaa cggacagcgg ggcgcctgct
     1081 catgcagccg caccaaccgg ccgtacagcc gacggagcgg ctcggcatgc ggactgctcc
     1141 agtctgtcag gcgctcgact tcgcggtaat ccagcgcctg tcgttcctgc aggccgacca
     1201 acgcggaatc aggcagcccc cccactgccg accggagcaa ccccaccagg gcgatgcggt
     1261 cgtgaggatt gtcgacgcac cggagcatgt tgaccaggtc gatcacttcc tgccggcggt
     1321 aaaaatgttt ctctccatcg atgatgtagg caatgccatg ccgccggagg gcttccaggt
     1381 agtgctcggc ctgagtcaat tttcgaaaga ggagcgcgat atgcccgggc ctaaggccac
     1441 tttccggccc ccccgcttcg cccccttgcc cggccggccg cagcaagccc gacaccatca
     1501 gtgcaatctg ttccgcctcc actcgcgtgg ccgctgccga atccaagtcg ccgtcgtctt
     1561 ccgatacgac caaccggagt tccacaccgg ggttgcgaaa ctgactcgat cgattgggtt
     1621 gcaccgtcag cggaacgttc ggcggctgaa tggcaggttg cgccaccagg agccggttga
     1681 atacgccgtt gaccagatcg agcacctgcg catggctgcg aaagttcgtg gccagttcgc
     1741 agcgcaacgc cccgctgcct tccagccgag cgacgacgtg gtcgaacgct tcgatatcag
     1801 cccgacggaa ggcatagatc gattgcttcg ggtcaccaac gatgaacagt tttcccgatt
     1861 cgagttccgt ctcccgccat gaaccggctt gtgtgccggg gcgctccgcc agatagagca
     1921 caatctcgta ctgcaccgga tcggtatcct gaaattcgtc gaccagcagc gcgcgatagt
     1981 cccgtttcaa ctgctcacga atgggcggat gatcgcgaag cagggtccgg gccttgccga
     2041 ccaagccgtc gaaggtgagc cagccggaat ccaggaacga cttccgcact gattgcacga
     2101 agggcgcgag cagggccagc agattctgca ggagttcatg atcgaccctg agcaaccgtt
     2161 tggcgatccg ctgcaatgac tggaccgcct cgtagtcgtc ttcctcccaa ccggtcgggg
     2221 cggcgccgag atctttggcc aacaattcct gcggctcgcg tgggaaagac cgcaccgcgc
     2281 ctaacccctc ggcaagcagg agggcgaaca ggtccgagac cgcggcgagc atcagttcga
     2341 ttttgcgtcg cttcgggcgg tcataccctc cgagcagacg ttccgcctgc gccttccgga
     2401 tggagaacca gtcacgcaat cccggattca gcccggtctc ggccacctgt tgcatcagcc
     2461 cattcagatc gatcaagtca ctgtgcaggc tgtaggccaa ctcgcgcagt tcgtccagac
     2521 tgaacgtatc caggagtatc cgccagcgac cgtgatcact ccccgccgcc cccaactcgc
     2581 gatccagcca caactcccac tcgctggtga agtgttcctc aaaccgtgaa ccgtcttcgt
     2641 ccgtttgaaa cgtcggggtc acgccggctt caatggggta caggcgcaag agatgcgcgg
     2701 caaaactatg cagcgttcca atctgcgcct tctcgacgtc acggagtgcg gcaccggccc
     2761 gggccacgat ctcatcggtc gtccagccat accgcatgcg caggtcggcg atcgaaaccg
     2821 tgccactacc ggccgcagga ttgtcgcggc cttccacatt caccaacgag cgaagccgct
     2881 ctcgtaaacg gagtttcatc tcagtggcgg ctttgttggt gaaggtcagg gccacgatgc
     2941 gcgacagatc caacggatcg gtccggcgca tgagcaggta caacaaccgg ttcaccagca
     3001 gtgtggtttt cccggttccc gccccggcga tcacgacgac attcctgtcg aacgtggtgg
     3061 cggctgcttc tctggccgcc tgatccggaa tctgcgccgg ttcagtcacg cgccaccttc
     3121 tgcgatcgca cgtcccgtaa cgtcctggcc tgcgaggacc gataggcccg ccaccagctc
     3181 ggttgatggg cgcggcggca ggcggttgag aattcacaat gggtgcaatg ggcgtccggg
     3241 acgatgaaat attgtgcggc gcgtatgcca tcgagcagca cctgcatcgt cctgctcaac
     3301 atcggaccgg agggtccctg ccaggccgaa gcggcaaagg acacacgctc aaccgcagga
     3361 tcggcatgcg ggagcaaata gagaaactcg acctgctccg gtagagcttt ctcccggtca
     3421 ccggacgacc cggcaggggt cattaaggca tagagcgcag gctgcagccg ttgagcacgc
     3481 agggccgctt gtaggagatt gcgatccttg gcctccacgc ggtcgttcgc tcgatactta
     3541 taatcgatga ccctcaccgc gccagacccc ggatgccgat ccacccggtc ccatcgtcca
     3601 cgcaacggaa tgtcctcccc gccagaacca tccggcaaaa gtccgttggc aaccaattcg
     3661 aattcagcgg gatggaaccc cgagaccaac gccattttcc gatcgagtgt gaccgtcgct
     3721 tcgatcaacc gccggaccga ctcctgtgcc aactcccagg tcaaggcata cccggtgcca
     3781 tgcgtcagag cataggcttc aaaggtctgc gcgacagcct gattcacctc cgcaatgatg
     3841 acggaggcag gcaacgtcgt cgccggccag ccctgttgga tcagcgccag ataacagaat
     3901 cgcaacgcgt catggcagag ttgccccatc gccggaggcg tgagttccat ggaggggacg
     3961 tggcgcaccg attcaagctt gagcacctgt ccggaaaaat attgaaaggg acaacgagca
     4021 tacgtttcca gcgccgtcgg cgaaaacccg cgttcagtga ccgtattcca atgggcgcct
     4081 gcatgttcaa gcatcccgtc gtacgcgctc agcgccggat gaccgctttc aatcactcct
     4141 tgcgccccca gtccatggga aaataaccac ccgtcacgtc caacctcctc caacagagct
     4201 gtcacgtcgc gtccctgcag taccatgctc acagccagtt cctctctcgt ctgcaggtgg
     4261 ggcgtgaaga gcggaagatc gacgcgatcc ggccaccgac gtggaagggc gaacagagaa
     4321 tcagggtccg acgggtgtgg caccaccccg accgcatcca gataaccgga gggcgccaag
     4381 ggacgaccgg cggcatccgc ccgttgatag gagagataca ggcgttcccc ggccgacgct
     4441 cgcaacagct cgaacagcag cgcctcctcc ccatagccct gcagcttctg gtcgatcttg
     4501 tagcccaggg tttcgctcag caccaggcgg tgccgatcac ggagaaatcc atcctcatgg
     4561 atataccggg gaaacagttt ttcgttcatt ccgacaagaa agagggcacg gaatcccacg
     4621 ccgcgggccg ccatcgcatc caacacctgc acgccgtggt gggaggacgg cgccagggca
     4681 cgggacgttc gcgccaatat ctccgcaaac gtttctgtcc attcgtccca ggtgaggtta
     4741 atgtcgaggc gatcgagcgc ccgcagctgg gcgaagaccc gggagagcgc ctcattcaca
     4801 tccgtggcat cgtcccactg cgccgtcgcg tcaatggacg tggagactcc gaggggaacg
     4861 gtcagatggg tctcagcgag ggagcagaag gcatcggtca gggtgccata cccgccctgc
     4921 tcaggaaggc gcttcacatc gtcgatcagg ggcgagaggc agccccacag cagacgcaac
     4981 tgcacgccat cgatcgagag cgactcgagc tcttccgtca cggggtcgtc ggcatcggac
     5041 gaccaggatt ccagacgacc caattgcgcc agccgtcgcc actcctcctc gccgcgggta
     5101 atcccaagcg ccagcaccgc caatcgccag aggtccggac gcggttcaac cccgccgcgg
     5161 ttggccgtca ggcggcgatt ccacgaggag gtaatcactt ccaacatggc cggtcgatgc
     5221 agcccgcttc ccttgagccg ggccaggtga agcagcgtct tcacggacgg ttcctgtaac
     5281 agcggcaccg tcgcgctgga gagaaacgga atgcgatgct ggtcgaaagt tcgtttcagg
     5341 gacgcctggt acggcacgag ggtgcgaccc accacaccga tttcgtcgaa cgcgaagccg
     5401 ttggtttcca ccagcgatag gatttgtttg cagaccagcg tcagctcatc gtcaatcccg
     5461 gcggcatttc gtacctcaac cgatacattt acttcctgag aagcatgcgc accatcaccg
     5521 gagggagccc cagctcgctc gtcgacccct ccggcaatcg ggtagaggtg ccgctccaga
     5581 aactgtcgcg caaaaccgta ggccggtcgt tcgctgagcg ggaaatatac cgtgaccggc
     5641 agggagatgc tgagcgattc caggagagtc agctgggttt gcgtcagatc gtacgacccg
     5701 tagtaccaga gaccgttgag actacggaga aacggagagg cgggtacaaa atcggtgacg
     5761 agcgcggcca agtcatccgg agatcccaca cccagcgcgg cactgccgtc ccgcagcgcg
     5821 gcatataagg taaacaatcc tttaagcttc tcaccgtctt caggcggaaa ctggccttcc
     5881 tccactgcgc gtaacgccag ggcagaatcc accgtggcat ccttgaggtc tcgcacgctg
     5941 gcccacaaag ccggccaggc tccggacggc agctgtggca aacgaagcgc ccccgtctga
     6001 ggcacgttgc gttgcccgag gtgctgaagc aggcgctcga aaaacgtgtc ggtgacgagt
     6061 tcaagctgcc gcaccgcacc agccgctcct gctgtagtgc ggcgctcgcg cagcaactgc
     6121 aacgccaact ggtgaaacga gagaatgtgg acgttcagta gtgccagccc ccgcttgacg
     6181 accagcagcc gcttgagcga gcgacgcaac tgatccgagg gcaccacaag cgcgactgcg
     6241 gcttgggggt cgccggactt gagaacacgc agatcgtgaa caagttgcga ttcgagtgtg
     6301 ggatgaaacg gaccggtgac gaggcggagc atggagaccg tgggccgtta atcgtaaagc
     6361 gcggagcgga aggccgcaga gagtatctcg gcaaccctcc gcacttcacg cgtgagaggc
     6421 gaagaacgcc gttgattgct agccggtttc cggaccgagc agctcaccgt tgcaatcgac
     6481 gcaggcccaa tcgccatcga tgaacttcat cgggtctccg cacgtcccgc attcaggcgg
     6541 cggtccctta tcgatcatag gaagaatcgg cagctcaaaa tcgtcatcat cgggaacggt
     6601 catatctctc cagtctcacg cgccctgcgg tttcatcttc agctgcaacg ccttctcggc
     6661 gctttgacga ggcggcaccc ccacgaacgt cagacggccg tcgataatcg tcgcgggcac
     6721 ggcccggacc gaatgccgat tcgccaattc ctgaccatcc ggggtcgtaa tatcgacctc
     6781 gcgatagcta aagctatact ttaccctaag ttccttccac aagcgtttcg ccgatggaca
     6841 ggctccgcag gtcggtgagt gcaataaggt aatattcggc atggacaacc cctctcctta
     6901 acgtaagcgg atcttaacac ccgcgcagag gaccgcgcaa gcaaacgcat gcctgacatt
     6961 gctcatcgag ccgtatatac tgagcgaggc atcattcaga accgcctcat ccacccatca
     7021 tcgtttgctg aggaatctat ggctctccac cctcgtgccg gacaaccggc acaaccggaa
     7081 caactggtca atctcgagaa acttgaacag gcctattatg gcgaagtacc ggacccgtcc
     7141 gatccccggc agcaggtgag tttcggcacc agcggacatc gcggttcctc ccttcgctgc
     7201 accttcaatg aagcccacat tcttgcgatc acccaggcca tctgcgaata ccgggctcag
     7261 gccggaacca ccggacccct gttcatcggc aaggataccc acgccctctc cgcaccggct
     7321 caacgcagtg cactggaggt actggccgca aatggtattc aggtccgcat ggatcaggct
     7381 gacgggtata cccccactcc ggtcatctcg cacgcaattc tgaccacgaa tcagggccgc
     7441 acgtcgggtc ttgcggatgg catcgtcatc accccttcac acaaccctcc ggaggacggc
     7501 ggcttcaagt acaacccgcc gcacggcggt ccggccgaca cggaggtcac gaaggcgatt
     7561 gagacgcgcg ccaatgcatt gctggcgacc aaactacacg gcgtcaaacg cgtcccttat
     7621 gaccaggcgt tgaaggccgc gtccacgacg cgacacgatt tcgtcggcca ctatctcgcc
     7681 gatctggcca acgtcgtgga tctccaacgc atcaaagcgg cgaagttgcg tctgggcgtc
     7741 gatccgttgg gcggcgccgc cgtggcctac tggcgtccac tggcagaacg gtatggattg
     7801 gacctgcaca tcgtcaacga gtccgtcgat ccgacctttc gtttcatgac gctggattgg
     7861 gacggcaaga tccgcatgga ctgctcttcc ccccacgcca tggcctcgct catcaaactc
     7921 aaagaccgct ttgatcttgc cttcggcaac gacaccgata ccgatcgcca tggcattgtg
     7981 acacccggga gcggtctgat gaaccccaat cactacctcg ctgccgcgat ctcgtatctg
     8041 tttacccatc gcccaggatg gaacgccggc gcaggaatcg gcaagaccgt cgtcagcagc
     8101 agcatgatcg accgggtagc gaatgactta caacgtccta tggtcgaagt gccggtcggc
     8161 ttcaagtggt tcgtgcccgg tcttcgagac ggttcgttag gattcggcgg tgaagaaagt
     8221 gcgggggcgg catttctccg tcgtgacggt accacatggg tcaccgataa ggacggtatc
     8281 atcatggatc tgctcgcggc ggaaatgctg gcggtcaccg gaaaagatcc ggcgcaactc
     8341 tatgcggact tgaccgcccg cctcggcgag ccggtctacg aacggatcga tgccccggcc
     8401 accagggcgc aaaaggccgc gctccagaaa ttctccccgc agcaggtgca gagtcgggaa
     8461 ctcgcgggag aaccgattac cgccatgttg acggcagcgc caggcaacat ggcttcgatc
     8521 ggagggttga aagtgacgac ggccaacggc tggttcgccg cgcgccccag cggaacggaa
     8581 gacgtctata aactctatgc ggaaagcttc aacggccagg cccatctcag gcggattcag
     8641 gaagatgcgc aagcgttgat caaacaggtc ttctccagcg ccggagcctg accggacacc
     8701 ggaacgttcc gcagaaaagt tcacatgtgt cgtcaaacgt gagacgtggg acgacagaac
     8761 gctcttttta gacacctgca acacgcgacg tttcacgtct tacggatttc tggacggtca
     8821 tttccagcct gcagtgagtt gttgccatcg ggcatgattc tgaagaccag ctatacttaa
     8881 gtcagtatgt ccagattctc tcacacgctg tgcaccctgc ttgtcggcgc cgccctgagc
     8941 gcctgcgccg caccggcatc ccagggtctc cgccaggcgg ttaccccgct tcctgactgc
     9001 tgccgcacaa cccaagccga ccttcgcaaa caggcgatcg tacaaaccgc cgtcaacctg
     9061 gtcggcgcga aaacgatcga gagccagggc cgccgcatct cttacgattg tgccggagtg
     9121 acccgagcca tctacctcgc ccacggcatc gacctctttg aaggaggtgc tgcagacggc
     9181 aaggccaacg gcgtgggtct gatctataac catcttcgca agcatggcca actccatcgc
     9241 ggtccggtcg tacaagccgg cgatctggtg tttttcgata atacctggga ctacaatggg
     9301 gacggtctcg taaacgatcc cttgacccat gtgggaatcg tggagagggt ggagagcaac
     9361 ggcacgattg tctttatcag ccgcgtggcc ggagcgatcg agcgctaccg gatgaatgtg
     9421 gcgcatcctc acattcaccg caccgccgac ggccgcttgc tcaatgacta catgcggcgg
     9481 aagcattggc gggatggcga acagacgccc tacctgaccg gggagttatt tgccgc
//
LOCUS       DAORRQ010000104         9223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_324_length_9223_cov_218.233421, whole genome shotgun sequence.
ACCESSION   DAORRQ010000104 DAORRQ010000000
VERSION     DAORRQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_9223_cov_218.233421"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..556
                     /locus_tag="PK224_19335"
                     /pseudo
     CDS             437..556
                     /locus_tag="PK224_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            921..1445
                     /locus_tag="PK224_19340"
     CDS             921..1445
                     /locus_tag="PK224_19340"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB17959.1"
                     /translation="MKHRQNSHPCFSLLAAAQRGFTLLEAMIAAGVLSVGLLGLAGLS
                     GMSLGKNVDANDMSRVTNIAADMAERIQNNRQRVLDYHNTNTSVACAQSANTQRMALG
                     DCTQWQALVANSGLTGATGLVTAARLDPDPTANPVTMNRFLVTVTVSWQTRQTDVSTA
                     RTKTAIFTTVVAPE"
     gene            1466..2569
                     /locus_tag="PK224_19345"
     CDS             1466..2569
                     /locus_tag="PK224_19345"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HRB17960.1"
                     /translation="MRRFDWNQQGFTLVELMAAVLITVVIVAATMTTVVTSNRANVVN
                     TQVADTQQNVRLAIDLLSRDIKLAGFNYNATDPATGSVGACNATIGAVVKPVGLLPQD
                     QTPTGADTGADSVSMVLPVMNTTGWTLTAAVGGTPNNKTYDNSISLSGAAMTEMVAQG
                     LVVGSTISIGGALSKTVQAVNATAIGFGTGNFVDGQFPVGTPVYLMQCVRYQVVANTP
                     ATCGSDTPCLVRNNVPLVDGVEDLQLAYACDGCNQAAPNPLFPDGVVDDQDASSTSGF
                     PTFTQGDFVSNSAWAIAPWTPDKIRLAQVGLVVRPANADDGLDEKGTKAVNTTGPVII
                     GDHNPSADTGYNASTYMQQRRRVMIRTIQPRNL"
     gene            2584..3216
                     /locus_tag="PK224_19350"
     CDS             2584..3216
                     /locus_tag="PK224_19350"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HRB17961.1"
                     /translation="MNLRQATTDRGQQDTCTGLRNQQGIALLTVMLMLLILTILGIAS
                     ITVTSMENRMAGFFRTTEAVVAAADSCEGLAANIIQQTLSPPGVLPASFIAPTGPVPT
                     ANATTLYAEIYGYDLPSPAPANTPAVNYADVASTAPNFVMTNLPGFTVNGDIDLLYTH
                     TKSGQGTGVAGTEHVYRITCMASNPATGSTSTVTSVYGCLDGDTCVKKIN"
     gene            3233..3562
                     /locus_tag="PK224_19355"
     CDS             3233..3562
                     /locus_tag="PK224_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17962.1"
                     /translation="MNNPVVIVMLGLLTLGQTMPSVPSSSSAETEQALEAMRFSTAGF
                     QAGVIDEVQGSSIRIDGRSYVLKSAVVVLTHEGEPLEIERIIPTSLVKFHLKEGHIDK
                     MVVTLPQ"
     gene            3640..8049
                     /locus_tag="PK224_19360"
     CDS             3640..8049
                     /locus_tag="PK224_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17963.1"
                     /translation="MNRRTFVAVMLVAGSLSAALLGSPDAVAVVSNADYTAVPAFVSN
                     ATTPNIIVVMDNSGSMSNRACESSSCGTLADGSTSTTTTWTNTTRYSGYFDSLRCYTY
                     NTTDTRFESGTGKTALATACPDTEWDGNFLNWATLRRFDAVKRAMIGGDCVSARAADG
                     TCPTSGTPALKTVRAQASGVSTERADVNSGGPGDTYVGRIPLADRSGSPATISIHVSD
                     AYFCVENDTSFNSNCGDSYSVRKYHLRVGYTTEPTGVIQQIGSKARFGLVEFKSSSEG
                     ARMLVGAGSRQSIDWADGDVETFTTNTAAMVDAVQESYPSTWTPLSEALYETARYVAQ
                     IQSTFTTGYVYPIAFSGGISNGVNFATSGAGSIGTSEITALVGTETCPAGYITSACGR
                     DPYFFGQNHTPPWSASSQVVACCRTFVIVFTDGEPTQDQNIPTALQDYAHAHHGQHCT
                     GSDGAAPPRTIDGTCNTHAATPFSDLLAEHKTSYADSGSHYLDDVAYWAHTTDLRPCS
                     GTTDGTIAGLSVTGHCIAGTQSLTVYSFFAFGNINGRGILAQAARLGGFEDSNNDGIP
                     QTSEWDKENNLTGAATPDGIPDAYFESSNVDDLQDKLLATIASILRRSASGSSVSVLA
                     TASTGEGALYQSYFYPSTIEPSTLSDVKWTGYTQALFIDTFGNTREDTNQDGRLDYKV
                     DKIIKTRFDSVSNSVKVDKYVDSDGDGLPNDQNSDGQVTVADCNPCGQTLSEILPIWE
                     AGKQLALKDSTTRTLLTWVDSDHDGVVDSGEQLAFTTANEPTLRPYLRATSSAEGTKI
                     INFVRGCDVGTCAEQATTRDRRLQVPTGSGSLKVWKLGDVIHSTPTVVAGPKDRHDAI
                     YGDQSYSAFLQKWYNRRQVAYVGGNDGMLHAFNAGYYHPGDDASASAPANTTEHGWFT
                     TGPADNSTGAPLGDELWGFVPYELLPQLEFLSRADYQHTYYVDLPLKVADVRIFTADT
                     DHPNGWGTILIGGFRMGGSCGACSAGTGAPPMTVNISGTDYTFYSSYFAMDITNPDAP
                     KLLWSFSSSNLGLTTSAPAVVRVNPAADAKASNTNAKWYMVVGSGPTGYDASVVQSAK
                     VFVVDLATGPGSGNSLVTSYSVGSWNSYIGDITAFDRDLDYRHDVVYFGRVIHDGALP
                     WRGKMYRLTMGAGGATAKFGTVTSPTQWGIASGSARVPTEMLDTFVSGAEMGPVATAP
                     SVTTDDAANVWVFAGSGRYYSKSDKTDVSTQYFVGLKDSVLNSGCNQSAGALNCMDND
                     LVDVSNATVCVVGVGDCGQASGTNQVSGVSGATTFTGLIGLVQSKDGWVTKLVEPANP
                     PTNPVPYGIGERAVSSPTVFGGVVFFPTFIPTNDICVSSGTSRLWALFYKTGSAYQEP
                     IIGTTTSGANQTVNKFGSLGEGLAFGIVVHMGSGRDGGSPFGLLINMSQGNFGDCATC
                     GFGGGSPGGLPSSNIGANVAIDPRSRYFSWTNM"
     gene            8034..9071
                     /locus_tag="PK224_19365"
     CDS             8034..9071
                     /locus_tag="PK224_19365"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRB17964.1"
                     /translation="MDEHVMIALGQAIHGRVGGLRPLAFPQATSLGLMALAVAVCCGC
                     SSSPQDGGVPGGGPKNNHPPTVRLVTIVPNPLILTGPITAHVAADDPDGTEPTKRFQW
                     IVNGVSVPGATEHELPTDHVKRGDTVALEVVVSDGQVESAPYRTEPVMVVNTPPLVSR
                     VTIEADSPDKGNRVLAKVEALDPDHDDIQYVYRWWRNDKQVKEGEENVLDTTGFGRKD
                     IVAVEVVARDQDATAAPARSVPIVLGNSPPQILSSPAALTNREQYEYVVQAKDVDGDS
                     ISYGLETGPPGMTIDKATGHVTWKVTPGVGGTHRIKIMVEDGQGGTAWQDFELSIPST
                     AQLLTVLPTQG"
BASE COUNT         1951 a         2524 c         2777 g         1971 t
ORIGIN      
        1 cacttgctct gataggccaa caaagaatat gttgcaacga ccacttgaat ccgcctagtg
       61 ccctcgcgtt cgccgtgtct cacgttttgc ccagggcgat actctccgtt caaggccgct
      121 cctgcttgtt aattatgcgc gggtgaacgt cagaaacagc ccgcttgatc tggcgcctat
      181 cctgggtgaa tttttccggt ctctgcccgc atcaagaggt tggtcgtggc ttctcttgcg
      241 cccttcctct ctcatcattc ctggctcacc agtaggtgaa gtacgccaga aatccgacaa
      301 aaattgtcat cgaagtgatc acggaacgct cggaattgta cgccgtccag tatgaatggg
      361 cttgatatat caatctgtta ggccatcata ccatgtcata tccatcggca tggcacttgc
      421 tcatttggct gttgccatga gtgtctctat ctctctccaa cggtctgctg ctgtccaccg
      481 ccagaggggc ttcaccctta tcgaggtgat gatagcggtc gcgattgtcg ggattctggc
      541 catggtggcc gtccccaact atcttcagtg gaatgcccgc tatcagctga aacaagccac
      601 aacggaattg gctggcagtc tcaacctggc ccggatggca gcgatgaatc ggaatcttgc
      661 cgtcacagcc acattggtcc ttgtgtcggg cagggtgaat gtcgatttcg gaggtgcgtt
      721 agcccctatt gtgttgcccc aggccgtcgt cggattcacg ggaggtccga cgattcaatt
      781 cacccagcaa gggttgagcg gggctgcggc gaatgttcct gtggcgctga cgtcccaaca
      841 ggggacgacc tattccgtgg tcgtcactcc ggcaggcaag gtgaactggt gtgcgcatgc
      901 gacgtgccca tagaggccca atgaaacacc gtcaaaactc acacccctgc ttcagtctgc
      961 tcgccgccgc acagcggggg tttacgctgt tggaagccat gattgcggcc ggtgtgctct
     1021 cggtcggact gttgggatta gcgggtctct cggggatgtc gctgggtaag aatgtcgatg
     1081 ccaacgacat gtcgcgcgtg accaatatcg ccgccgatat ggcggagcgc attcaaaata
     1141 accggcagcg tgttttggac taccataaca ccaacacgag cgtggcatgc gcgcagagcg
     1201 cgaacactca acgcatggcg ttgggagatt gcacccaatg gcaggcgctc gtggcgaatt
     1261 ccggtctcac gggtgccact ggacttgtca ccgcggcccg ccttgatccg gaccccaccg
     1321 cgaatccggt gacgatgaac cggttccttg tcacggtgac cgtcagctgg caaaccagac
     1381 agacggatgt gagcacggcc cggaccaaga ccgcgatatt taccacggtg gtcgcgccgg
     1441 aataggcgta gcctggagca tgacgatgcg tagattcgac tggaaccagc agggcttcac
     1501 tctcgtggaa ctgatggcag ctgtcctgat cacggtggtg attgtggcgg cgacgatgac
     1561 cacggtggtc acttcgaatc gcgccaatgt cgtcaacaca caggtcgcag acacgcaaca
     1621 gaatgttcga ttggccatcg acttgctcag ccgggatatc aaactggccg gctttaatta
     1681 caatgcgacc gatccggcaa ccggatctgt gggcgcgtgc aatgccacca tcggcgccgt
     1741 cgtcaagccg gttggactgc tgccccaaga ccaaacgccg acaggtgcgg acaccggggc
     1801 tgatagcgtg tcaatggtgt tgccggtgat gaatacgaca gggtggacac tgacggcggc
     1861 ggtcggtgga acgccgaaca ataagacgta tgacaacagc atcagtcttt ccggagccgc
     1921 tatgacggag atggttgcac aaggccttgt ggtcggatcg acgatttcca tcggcggtgc
     1981 attgtcaaag acggtgcagg ctgtcaatgc cactgcgatt ggcttcggta ccggaaactt
     2041 cgtcgatgga caattcccgg tcggcacgcc tgtgtatttg atgcaatgcg tgcggtatca
     2101 agtggtggcg aatacgccgg cgacctgcgg cagtgacacg ccttgcctgg tgcgaaacaa
     2161 cgtgccgctg gtcgatggtg tggaagactt gcaactcgcc tacgcctgtg acgggtgcaa
     2221 tcaggccgcg cccaatccct tgtttccgga cggggttgtc gatgatcaag atgcctcctc
     2281 aacgtcggga tttcccacct tcacgcaggg agattttgtc tccaacagtg cgtgggcgat
     2341 tgccccgtgg acgccggata agatcaggct ggcgcaagtg ggcctggtgg tcaggccggc
     2401 gaacgcggat gacggtcttg atgaaaaggg aaccaaggcc gtcaacacca ccgggcctgt
     2461 gatcatcggc gaccataatc cctctgcaga tacggggtat aacgcgagca cttatatgca
     2521 acagcgacgg cgcgtgatga tccgcaccat tcagccgaga aacctgtaac gagagacgct
     2581 gtgatgaatc tgcgacaggc cacaaccgat cgaggccaac aggatacgtg cacagggctg
     2641 aggaaccagc agggcatcgc actgctcact gtgatgttga tgttgttgat tctcaccata
     2701 ctcggcatcg cctccatcac tgtcaccagt atggaaaatc gaatggcggg atttttccgc
     2761 acgaccgagg ctgtggtagc ggctgccgat tcgtgtgaag ggctggccgc caacatcatt
     2821 cagcagacgt tgtcgcctcc aggggtgctc ccggcttcgt tcatcgcgcc gacggggccg
     2881 gttccgacgg ccaatgccac cacattgtac gcagaaattt atggctacga tctgccgtcg
     2941 ccggctccgg caaatacacc ggctgtcaac tatgcggatg tggcgagcac cgcaccgaat
     3001 tttgtgatga cgaaccttcc cggattcacg gtcaacggcg acatcgatct cttgtatacg
     3061 catacgaaga gtgggcaggg tacaggagtg gccggcacag aacatgtcta tcggattacc
     3121 tgcatggcga gtaaccccgc gaccggttcc actagcacgg tgacctcggt gtacggctgt
     3181 ttggacggcg atacctgtgt caagaaaatc aattgagaga gggagcatgg cgatgaacaa
     3241 cccagtagtt attgtgatgc tcggcctgct gacactcgga cagacgatgc cttccgtgcc
     3301 gtccagcagc agcgcagaga cagagcaagc actggaagcg atgcggtttt cgacggccgg
     3361 gtttcaggcc ggcgtgatcg acgaggtgca gggatcaagc attcgcatcg atgggcgtag
     3421 ttatgtgttg aagtctgcgg tggtggtgct cacccacgaa ggggagccgc tcgagattga
     3481 gcggatcatt cccacttcgc tcgtaaagtt tcatctgaaa gagggccaca tcgacaagat
     3541 ggtggtcacg ttgccgcagt aacgcatgaa aggtcttgtc tcaactcgta gccgagaatc
     3601 gacggctgtt gtcctgtttg agataaggag gaagaacgca tgaatcgccg aacctttgtc
     3661 gctgtgatgt tggtggccgg cagcctgagt gctgcgttgc ttggctcccc tgatgccgtc
     3721 gccgtcgtgt ccaacgccga ttacacggct gtgccggctt tcgtgtcgaa cgcgacgacg
     3781 ccgaatatca ttgtggtgat ggacaactcg ggcagtatga gcaaccgggc atgtgaatcg
     3841 tcgtcgtgcg ggacgttggc ggatgggagc acgtcgacta cgaccacatg gaccaatacg
     3901 acacggtact ccgggtactt tgattcgctg cgttgctata cctacaacac aaccgatact
     3961 cgattcgaga gcggaaccgg caagaccgca ttggcgacag cctgtccgga cacggaatgg
     4021 gacgggaatt tcttgaactg ggcgacgttg cgccggttcg atgcggtgaa gcgagcgatg
     4081 atcggcggag attgtgtcag tgccagggcc gcggacggaa cctgcccgac aagtgggaca
     4141 ccagccctga agaccgttcg tgcacaggcc agcggagtaa gcaccgaacg tgccgatgtc
     4201 aacagtggag ggcccggcga cacctacgtg ggccgcattc cattggccga tcgaagtggt
     4261 agtccggcga cgatttctat tcatgtatcg gatgcctatt tttgtgtcga aaacgataca
     4321 agcttcaata gcaactgcgg ggatagctac agtgtccgaa agtatcattt aagggtcggg
     4381 tacacgacgg agccgaccgg ggtcattcag cagatcggca gtaaagcgcg ttttgggctg
     4441 gttgagttta agtccagcag cgaaggcgcg cgtatgctgg tgggagctgg atcgcgccag
     4501 tcgatcgact gggcggacgg cgacgtggag acgttcacga cgaacacggc agccatggtc
     4561 gatgcggtgc aggaatccta tccttcaacg tggactccct taagtgaggc gttgtatgaa
     4621 accgcgcgct atgtcgcgca gattcaatcc acttttacga cgggctatgt ctatccgatc
     4681 gcgttctccg gtggcatttc caacggcgtc aattttgcca cgagtggcgc cggttccatc
     4741 gggacgagcg aaataacggc attggtcggt actgaaacgt gccccgccgg atatatcacg
     4801 agtgcctgtg gccgagaccc gtattttttt ggacaaaacc ataccccgcc atggtccgcc
     4861 agctctcagg ttgtggcctg ctgtcggacg tttgtgatcg tctttacgga cggcgaaccg
     4921 acacaagacc agaatatccc gacggctctg caagactatg cgcatgccca tcatgggcag
     4981 cattgcaccg ggagcgatgg cgcagctcct ccgcgtacca tcgatggcac ctgcaataca
     5041 cacgcagcca ctcctttcag tgatctgttg gcagagcaca aaacgagtta tgctgatagc
     5101 ggcagccact acttggatga cgtcgcctat tgggcccaca cgaccgacct ccggccttgt
     5161 agcggcacga ctgatggcac catcgcaggg ttatcggtca ccgggcattg catcgcaggg
     5221 actcagagtc tgacggtgta ctccttcttt gccttcggta acatcaatgg tcgtggaatc
     5281 ctggcccaag cggcccggtt gggagggttt gaggattcca acaatgacgg catcccacaa
     5341 acgagtgaat gggataaaga gaacaatctc accggcgcgg cgactcccga cggtatcccg
     5401 gacgcgtatt ttgaatcttc gaacgttgat gatcttcagg acaagctgct cgccaccatt
     5461 gcaagcattc tcaggcgaag tgcgtccggt tcatccgtat ccgtgctggc cactgcctcg
     5521 accggcgaag gggctctgta ccaatcctat ttttatccca gcacgatcga accctcgacg
     5581 ctgagcgatg tgaaatggac cggctatacg caggccctgt ttatcgatac cttcggcaat
     5641 acccgtgagg atacgaacca ggatggtcgg cttgattaca aggtcgacaa gatcatcaag
     5701 actcgttttg attcggtgtc gaactcagtc aaagtcgata agtacgtcga cagtgacggc
     5761 gatggattgc cgaacgatca gaatagcgac gggcaggtca cggtggcgga ctgcaacccc
     5821 tgcgggcaga cgttgagcga gatcctgcct atttgggaag ccgggaagca actggcgttg
     5881 aaagactcca ccactcgtac cctccttacc tgggtcgatt ccgatcatga cggcgtggtc
     5941 gattccggcg agcagctggc gttcacgaca gccaacgagc cgacgctccg accatacctt
     6001 cgtgccacgt cgagtgccga agggaccaag atcattaatt ttgtgcgtgg ttgcgatgtg
     6061 gggacatgcg cggaacaggc gacgacgaga gatcgccgtc ttcaggttcc gaccgggagc
     6121 gggagcttga aggtgtggaa actcggggat gtcattcact ccactccgac ggtggttgcg
     6181 ggtccgaagg accggcatga tgctatctat ggcgatcaga gttattcggc cttcctccag
     6241 aagtggtaca accgcaggca ggtcgcctac gtgggcggaa acgacggcat gctgcatgcc
     6301 ttcaacgcgg gttattacca tcccggcgat gatgcgagtg cctctgctcc cgcaaatacg
     6361 accgaacacg gctggtttac gactggccct gcggacaatt ccaccggagc tccattaggg
     6421 gatgagttgt gggggttcgt gccctacgaa ctccttccgc agctagagtt tttgagtcgc
     6481 gccgactatc agcatacgta ttatgttgat ttgcccctca aggtagcgga tgtccgcatc
     6541 ttcactgcgg ataccgatca tccgaacgga tggggtacga tcctgatcgg cggattcaga
     6601 atgggtggaa gttgcggcgc ttgttcggcg ggaaccggtg cgccgccgat gacggtcaat
     6661 atcagtggca cggactacac cttctacagt tcgtactttg cgatggatat cacgaatccc
     6721 gatgctccca aattgctctg gtcgttcagc agttccaatt tggggctgac gaccagtgct
     6781 cctgccgtgg ttcgcgtgaa tcccgcggcc gatgccaagg ccagcaatac gaacgcgaag
     6841 tggtacatgg tggtcgggtc tggcccgacc ggatatgacg ccagcgtagt tcaaagtgca
     6901 aaagtctttg tcgtcgacct tgccacgggc ccaggtagcg gcaacagcct cgtgacgtcg
     6961 tattcggtgg ggtcctggaa ttcgtatatc ggagacatca cggctttcga tcgagatctc
     7021 gattaccgtc acgatgtcgt gtatttcggg cgagtgattc atgacggagc gcttccctgg
     7081 cggggcaaga tgtatcgttt gaccatggga gcagggggag ctacggccaa gttcggtacg
     7141 gtcaccagtc cgactcagtg gggcatcgcc tcgggaagtg ctcgcgtgcc cacggagatg
     7201 ctggatacgt tcgtatcagg ggcggagatg ggccccgttg cgaccgcgcc ttccgtgacc
     7261 acagacgatg cggctaacgt gtgggtcttt gcgggcagcg ggcggtatta cagcaaatcc
     7321 gataagaccg acgtgtccac gcaatacttc gtggggttga aagactcggt cctcaattcc
     7381 ggatgcaatc aatcagccgg cgcgctcaac tgcatggaca atgatcttgt ggatgtctcg
     7441 aatgccaccg tctgtgtggt gggagtcggc gattgtggtc aggccagtgg aaccaaccaa
     7501 gtctccggcg tgtccggtgc cacgacgttt accggtttga ttggattggt tcagagtaaa
     7561 gatggttggg tgactaaact ggtcgaaccc gccaacccac cgactaaccc agtcccttac
     7621 ggcatcggcg aacgtgctgt gagtagccca acggtattcg gaggggtggt gtttttcccc
     7681 acctttattc caacgaacga catctgcgtg tcatccggaa cgagccgact ctgggcactc
     7741 ttctataaaa cggggagtgc atatcaagag ccgatcatcg gaacgactac ctcaggagcg
     7801 aatcaaacgg tcaataagtt cggctctctt ggagaaggat tggcgttcgg tatcgtagtc
     7861 catatgggct caggacggga cggcggcagt ccgttcggcc tcctgatcaa tatgagtcag
     7921 ggaaacttcg gtgactgtgc aacctgcggg ttcggtggag ggtctcccgg aggtcttccg
     7981 agctcaaaca tcggggccaa tgttgcgatt gatccacgga gccggtactt ctcatggacg
     8041 aacatgtgat gatcgccttg gggcaggcca tacacgggag ggtggggggg ctccgtcccc
     8101 ttgcctttcc tcaggcgact agtctgggct tgatggccct cgctgttgct gtctgctgcg
     8161 gttgttcgtc atctcctcag gatggcgggg ttcccggggg cggcccgaaa aataaccacc
     8221 ctccgaccgt ccgcctcgtg accatcgttc ccaatccctt gatattgact gggcccatca
     8281 ctgcacatgt tgcggccgat gacccggacg gtaccgaacc gaccaagcga ttccagtgga
     8341 tcgtcaacgg ggtttcggta cctggtgcga cggagcatga actccctact gaccatgtga
     8401 agcgcggcga cacggtcgcg ctcgaagtgg tggtgtctga tggccaggta gagagcgctc
     8461 cctatcgcac ggagccggtc atggtcgtga atacgccccc tctcgtgtca cgggtgacaa
     8521 ttgaggcaga ttcccccgat aaggggaatc gggtcctcgc caaggtcgag gcgttggacc
     8581 ccgaccatga cgatatccaa tacgtctatc gctggtggcg taacgacaag caggtgaaag
     8641 aaggcgagga aaacgttctt gatacaaccg ggtttggccg aaaggatatt gtggcggtcg
     8701 aagtcgtcgc aagggatcag gatgcgacgg ccgcacctgc tcgatcagtg ccgatcgtcc
     8761 tcgggaattc accgccacag atcctctcaa gtccggccgc gttgaccaat agagagcaat
     8821 acgagtacgt cgtccaagcg aaggatgtcg atggcgatag catcagctat gggctcgaga
     8881 cagggcctcc agggatgacg atcgacaagg cgacgggcca cgtgacctgg aaagtgacgc
     8941 cgggtgtcgg gggaactcat cgcatcaaga tcatggtgga agatgggcag ggtggcacgg
     9001 cctggcagga ttttgagctc tcgataccct ctactgccca attgctgacg gtcctcccca
     9061 ctcaaggcta gtcggttccg gtgggcctcc ttattttctg ttctgtcccc tccgaccttg
     9121 ttccggactt tcccggtttg ttagaatctc cctatcgtcc tgacgaaagg agttttcgac
     9181 accggcgtgc gcaagatcaa actacgggaa ggcacgaata ccg
//
LOCUS       DAORRQ010000105         8955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_331_length_8955_cov_19.052697, whole genome shotgun sequence.
ACCESSION   DAORRQ010000105 DAORRQ010000000
VERSION     DAORRQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_331_length_8955_cov_19.052697"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK224_19370"
     CDS             <1..1063
                     /locus_tag="PK224_19370"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRB17965.1"
                     /translation="SRVASFHFFNHRKDDIMATRQLVPDRIMQLGFGFWGSKTLLSAV
                     ELGLFTELAKRSLDAKALAKRLEVHPRSARDFFDALVALGMLKRVGTRYANTSEAALF
                     LDRAKPSYVGGILEMANVRLYPFWGSLTEGLRTGKPQNEVKTGEDFFGTLYADPQRLE
                     GFLKAMTGLSQGTARAIAAKFPWKKYRSFVDIGCAQGGVAAEIALAHKHLSGQGMDLP
                     VVRPIFEAYAKSRKLEKRLTFHAGDFFNDALPKADVLIMGHILHDWDLNEKMMLLRKA
                     YAALPQGGALIVHEALIDDARKQNAFGLLMSLNMLIETPGGFDFTGADCIQWMKKAGF
                     KRTKVEKLAGPDSMVIAIK"
     gene            1091..1900
                     /locus_tag="PK224_19375"
     CDS             1091..1900
                     /locus_tag="PK224_19375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB17966.1"
                     /translation="MTALSPRVSPRWPLPAQDDWSTPFAEVLLGQLDLRPGLTILDIA
                     SGHGIPAFYLAEQVGETGTVIGIDASRSQVASARAIQRGELPWLRFDCQDMRAIPASL
                     PTFQRITGNLSVMFLRPNRFEAMRGVIDHLAPGGQVVLTFPSLGTFDSIWRRIDQEMG
                     RHGLVIERERLAAHVAERPSAAEARGWLERLELERIAVVEQPLEVVSGSGQRFLQHPL
                     LRGGFLDDAYECFEDQRLAEEVMTRVSLDLESMTPLIAQRCAISGWKRSVS"
     gene            1923..2456
                     /locus_tag="PK224_19380"
     CDS             1923..2456
                     /locus_tag="PK224_19380"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HRB17967.1"
                     /translation="MEVRVNRMLTGLMGLALLSGCSSSLPARYIQQAESDVTLTSLMK
                     SPGAYHGKTVILGGVVVDQQQDGQRLWLHVRNRPLDKDYRPHRPIVNEGPEAGYYWIS
                     VPNAVTLPPKWKQWARVTVVGRVTDPKTAAPSTEPVLNLVFMRGWTMGQAQQGGAREE
                     SVGANNLLSVPEGLHGE"
     gene            complement(2548..3291)
                     /locus_tag="PK224_19385"
     CDS             complement(2548..3291)
                     /locus_tag="PK224_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRB17968.1"
                     /translation="MLDWLANPEIWIALGTLTALEIVLGIDNIIFLSVLVGRLPEHQR
                     AVARQVGLGLAMMARLGLLFSISWVMGLTHPWVTILGHGVSGRDLILVGGGLFLMAKA
                     THEIHNSLEGIEGGETPAAAASLGMVLVQIALLDIVFSLDSVITAVGLVEHVSIMAIA
                     IILAVVVMLMAAKAIGDFVDKHPTIKILALSFLILVGVTLMVEGFDVHVPKGYIYFSM
                     AFSVTVEMLNIRMRKKRAAPVKLHSRYAE"
     gene            complement(3503..4567)
                     /locus_tag="PK224_19390"
     CDS             complement(3503..4567)
                     /locus_tag="PK224_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17969.1"
                     /translation="MTRMATVPADTAQSYRTHPGILACCLVLTLMQGCTGGFHVRAGD
                     DDDDITESHCVPTTSPPTQVVTLPATGTTYRIDGLTKSSRTEPVTPSRFSPRAREIAV
                     IIGVHDLLVESQLLEDEMAGGMEGALLHSLAVRQELSDRLLLALFEANSIAAELECEK
                     ARTEDLASQLEEVRNDIQQGRTIMAILADSISGVFAGTFLLLGSEILSGLIDLSGSVA
                     ALGFGVAALGGDQAYEFKHGRNLMADVWQGGTISRQFPQPVWQYLNGPVRPNGKRSRR
                     DNIIGTWRTRLGKPGSKEEQRLTGLYFGRGGIYRINELRHRGEMLGLLKTHVNLMTQD
                     LNLLFLETLQHRSSRPEQPI"
     gene            complement(4971..5819)
                     /locus_tag="PK224_19395"
     CDS             complement(4971..5819)
                     /locus_tag="PK224_19395"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB17970.1"
                     /translation="MTTVSGQHVSDYMHRQLEVVPQDTSVVTVATQMRTRSVGSVLIE
                     CFDRPHNDCRIAGIVTETDLVAKVLAPGRVPSRTSMADIMSSPLITIAPTRPMVDASH
                     LMQSKNVRHLVVTEGTDVLGIISIRDLVRYFVDADGGPVQALTNVYRPLSVLMKTAIE
                     TIGSDETALEAAQRMADKHIGALFVMEADELVGIITEGDLVRKLLAYQLDPQTLRVGA
                     LMNSPLLDIDINRTIRDASERMSAKRIRHLAVTEHEKVVGVLSIRDLVKMVAVRDRPD
                     FLKRES"
     gene            complement(5912..8710)
                     /locus_tag="PK224_19400"
     CDS             complement(5912..8710)
                     /locus_tag="PK224_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRB17971.1"
                     /translation="MPELTALEVCRLAPSQVYRALATTPQGLSSDDVRRRTLRYGPNS
                     LQALRGMPLISRFARQFTHFLALLLWVAAGLAFVADALHSGEGMATLGWAILGVILIN
                     AIFAFLQEYKAERAVQALRSMLPAQAWVLRDGQQQQIARSELVPGDVLVLEEGEQIPA
                     DARLTAAVGMRVDNSSLTGESKPQRRSADPITDGHPLDIANLAFAGTTVLSGHGQGVV
                     FATGLNTEFGKIAHLTTTVQTGLSPLQQEIVKVTHVVAGLSLLMGLVFFTIGVGMGLG
                     FWASTIFGIGIIVANVPEGLLPTVTLALAMGSQRMAARKALIKHLASVETLGCTTVIC
                     TDKTGTLTENRMRLDRLYVDDLVVESREGCLFTRNRLITAPESERWRPLFDAIVHCNN
                     AKRTRHPDGRSRTTGDPTETALLEFAADHGLLHRPPLRRMGEMPFDADRKRMTTLHWS
                     EGRLLAFTKGAPESVLPLCTRQQGSSTPAELTVDGRKKVLAQSQAFAQQAYRVLAVAM
                     REVERGVDQLEADTVEQGLTFLGLVAMMDPPHREVPDAIKKCRRAGVRVMMITGDHPL
                     TALAVARKIGLAPETIPTEPGHFVPVIEGAHIDTFSDDQLRQLLTPTAPGEPDPIFAR
                     MAPRHKMRIVSALKEMREVVAVTGDGVNDAPALKTADIGIAMGIAGTDVAKETASMIL
                     LDDNFATIVHAIEEGRAVYTNIRKFVTYVLASNVPEIVPYLGFGLSSMPLALTIPQIL
                     AVDLGTDMVPALALAAEPPEGGVMDDPPRPRTERLLSWDVILRAYAFLGLIEAGIAMG
                     GFFLYLYSQGWSWAAPLDWTSPLYKEATTVTFAGIVAAQVANVFACRSDRISAFRLGW
                     FSNPLILVGVAVELTILLVMTYSPVGHLVLGTASLPAWIFGPLALGAIGLLLADELQK
                     FVRGRMGARLSLQPDRGT"
     gene            complement(8703..>8955)
                     /locus_tag="PK224_19405"
     CDS             complement(8703..>8955)
                     /locus_tag="PK224_19405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRB17972.1"
                     /translation="AAEQVVENLRDRFLAEGCAVTTQVKVDHVTDTIIQQATSGKVDL
                     LVAGSRGLTGSERLQLGTVSETLLKYAPCSVLIVRGWRA"
BASE COUNT         1727 a         2594 c         2861 g         1773 t
ORIGIN      
        1 cagccgcgta gcctcttttc attttttcaa tcatcgaaag gacgacatca tggccactcg
       61 gcaattggtt cctgatcgga tcatgcaact cgggttcggg ttttggggat cgaagacttt
      121 gctgagcgcg gtcgaacttg gtctgttcac ggaactcgcc aaacggtcgc tcgatgccaa
      181 ggctcttgcg aagcgactgg aagtgcatcc gcgaagcgcg agggattttt tcgacgcatt
      241 ggtcgccctc gggatgctga agcgggtggg cacgcgctat gcgaacacct ctgaggccgc
      301 gctctttctg gatcgtgcca aaccgtccta tgtcggcggc atactcgaaa tggccaacgt
      361 caggctctat ccattctggg gatcgctcac ggaagggctc cggaccggga agccgcaaaa
      421 tgaagtgaag acaggagagg atttcttcgg cacgttgtac gccgatccgc agcggctgga
      481 agggtttctc aaagccatga ccggactcag tcaaggcacg gcgcgggcca tcgcggcgaa
      541 gttcccttgg aagaaatatc gttccttcgt cgacatcggc tgcgcgcagg gcggtgtggc
      601 ggctgagatc gcgctggccc acaagcatct gtccgggcaa ggcatggatc tgccggtcgt
      661 gcgtccgatc ttcgaggcct atgcgaagtc caggaagctg gagaagcggc tcacgtttca
      721 cgcgggggat ttcttcaacg atgcgcttcc gaaggccgat gtcctcatta tgggacacat
      781 cctccatgac tgggatctca acgagaagat gatgctgttg cgtaaagcct atgcggccct
      841 gccccagggc ggggcgttga tcgtccatga agcgctgatc gacgatgccc gcaaacagaa
      901 tgcgttcgga ttgttgatga gcctgaacat gctgatcgaa acgcccggcg gattcgattt
      961 caccggtgcg gattgcattc agtggatgaa gaaggccggc ttcaagcgaa ccaaggtgga
     1021 aaaactggcg gggccggatt caatggtgat tgcgatcaag taatgccgtc gtgtcggcga
     1081 aaggaatgtc atgaccgcac tgtccccgcg cgtgtctcca cgttggcctc ttcccgcgca
     1141 ggatgactgg tcgactccgt ttgccgaggt cttgctgggg cagctggatc tgcgtccggg
     1201 cctgacgatc cttgatatcg cttcgggaca cggcattccg gccttctatc tggcggagca
     1261 ggtcggtgag accggcaccg tgatcggtat cgatgccagt cgaagccagg tcgcgagtgc
     1321 gcgcgccatt cagcggggcg agcttccctg gctgcgattc gactgtcagg acatgcgtgc
     1381 catacccgca tcgctcccca cctttcaacg tatcaccggc aatctctccg tcatgttctt
     1441 gcgcccgaac cgctttgagg cgatgcgtgg agtgatcgac catctggcgc cgggcggtca
     1501 ggtggttctg acgttccctt ccctcggcac gttcgactcc atctggcgcc ggatcgatca
     1561 ggaaatgggg cgacacggac ttgtcatcga gcgggaacga ttggcagccc atgtggcgga
     1621 gcggccatcg gcggcagaag cgcgtggatg gctggagcgg ctcgaactgg agcggatcgc
     1681 tgtcgtcgag cagccgttgg aggttgtcag tgggtccggc caacggtttc tccagcatcc
     1741 gctcctccga ggcgggttcc tcgacgatgc ctatgaatgt ttcgaggatc agcggctggc
     1801 ggaggaggtg atgacgcgcg tctcactgga tttggagagc atgactccgt tgattgcgca
     1861 gcgatgtgcc atcagtggtt ggaagcgcag cgtatcatag tgtcgaaaag cgggaggttg
     1921 atgtggaggt acgggtcaat cgaatgctga cgggactcat gggtcttgcg ttgttgtcgg
     1981 gatgcagctc gtccttgcct gctcgataca ttcagcaggc ggaatccgac gtcacgttga
     2041 cttccctcat gaagtcgccg ggagcctatc acggtaagac agtcattctg ggaggggtcg
     2101 tggtggatca gcagcaggac ggccagcgtt tgtggctcca tgtcagaaat cgcccgctgg
     2161 acaaggacta ccggcctcat cgtccgatcg tcaacgaagg gccggaggca ggatactact
     2221 ggatctcggt gccgaacgcc gtgacattgc cgcccaaatg gaaacagtgg gcgcgggtga
     2281 cggtggtcgg gcgggtcacg gatccgaaga cggccgcgcc ttcaaccgaa ccggtgctca
     2341 acttggtatt catgcgaggg tggaccatgg ggcaggctca gcaaggcggc gcgcgggagg
     2401 aatcggtggg tgcgaacaat ctgctttctg tgccggaggg ccttcacggg gagtgaggga
     2461 tcctgcacgc ttcgaggcgt cgcctccacc tgccgatgcc ggggccgatt caccgtcccc
     2521 ggccgaccgg cagtgttatt gccgccgcta ctccgcataa cgactgtgga gtttgaccgg
     2581 cgcagcccgc ttttttcgca tgcgaatgtt gagcatttcg accgtgacgg agaaggccat
     2641 ggagaagtag atatagccct tcggcacatg cacgtcgaac ccttcgacca tcaaggtcac
     2701 gcccacgaga atcagaaacg agagggccag aattttgatc gtggggtgtt tatccacaaa
     2761 atccccgatg gctttggccg ccatcaacat caccaccacg gcgagaatga ttgcgatcgc
     2821 catgatcgag acatgttcga ccaggccgac cgccgtaatc accgaatcca acgaaaagac
     2881 gatatccagg agcgcgatct gaacaagcac catccccagg ctggcagcgg cggccggggt
     2941 ctcgccgcct tcgatcccct ccaggctgtt gtggatttcg tgcgtggctt tggccatgag
     3001 gaacaggccg ccgccgacta gaatcagatc ccgcccggac acgccatggc ccaagatcgt
     3061 cacccaggga tgggtgagtc ccatgaccca ggaaatggaa aacaagagtc ccaggcgcgc
     3121 catcatggcc aggcccaggc ccacttggcg tgcgacggcg cgctgatgct cggggagtcg
     3181 tccgacgagg acggagagaa agataatgtt gtcgatcccc agcacgattt ccagcgcggt
     3241 caaggttccc agggcgatcc atatttcagg attggcaagc cagtccaaca tcagcggctc
     3301 cattcgtgaa agagagggcg agcgggtcga tctgtcatgc gaggatgtgt agcatacgat
     3361 acgtatcgag gaagtctacc gttccaagcg ggtctgcgca atgaaaacca gtcgataggc
     3421 ggtgttgggt gagcgaatgg atcgaccgcg ggggtggctg tgggaacggc cgttccttca
     3481 ggaagaatcg gatccgtcgg ggctagatcg gctgttcggg acgggaagag cggtgttgga
     3541 gcgtttcgag gaacagcagg ttcaggtcct gggtcatcag gttgacgtgg gttttgagca
     3601 ggcccagcat ctcgccgcga tgccgcagct cgttgattcg atagatcccg cctctgccga
     3661 aatacagtcc ggtcagacgt tgctcttctt tcgagcccgg tttgccaaga cgggtgcgcc
     3721 aggtcccaat gatattgtct cgacggctcc gtttaccgtt cgggcggacc ggcccgttca
     3781 gatactgcca gaccggctgc gggaactgcc gactgatggt tccgccttgc cacacgtctg
     3841 ccatgagatt ccgcccgtgc ttgaactcat aggcctggtc gccgcccaac gcggccacgc
     3901 cgaaccccaa cgcagccacg cttccggaca gatcgatcag gccggagaga atctccgacc
     3961 ccagcagcag gaacgttccc gcaaagacac cggaaatgga gtcggccaga atggccatga
     4021 ttgtccgtcc ctgctggatg tcgtttcgga cttcctccag ctgcgacgcc agatcctcgg
     4081 tccttgcctt ctcgcattcc aattcggcgg cgatgctgtt ggcttcgaag agcgcgagga
     4141 gcaggcgatc ggacaattcc tgtcgtaccg ctagggagtg tagcaatgcg ccttccattc
     4201 ctcccgccat ctcatcttcc agcaattggg attccacgag gaggtcatgg acgccgatga
     4261 tgacggcgat ttcccttgcc cgtggtgaga atcgtgaagg ggtgacgggt tcggtgcgcg
     4321 aggatttcgt gaggccgtcg attcgatagg ttgtgcccgt ggccggcagg gtcacgactt
     4381 gggtcggcgg actggtcgtg gggacgcaat ggctttcggt gatgtcgtca tcgtcatcgc
     4441 ctgcccggac atgaaatccg ccggtgcagc cctgcatcag ggtgagcacc aggcagcaag
     4501 cgaggatgcc ggggtgggtt cgatatgact gagctgtgtc cgccggcacg gtagccattc
     4561 gcgtcatgaa cggtgtctgc cgaaagggat gtcaatcggt acgcacgcat gcagtcagca
     4621 ctgcgcctgc ccgcccggga ccctctgcgt tccggcaaca agacatgagc tcccgcgcag
     4681 ataggttctc tcccagcctg aaggccgcag atatcctgtc atcgacggta ccggagaaaa
     4741 atgaagcgag catgaagggc ggatgaacgg tcgatgaatc cggaaccgga ccggacggcg
     4801 cagatctcga tggcggcagg accgggcagg gctgagcgat gacggagcac ggccgcagat
     4861 aagagtgtat ggcatatggc tcttagcgta tggcctaaaa tcgagagtgg gcgtctctac
     4921 tctcagctat acgccaccag ccatacgctc tgaccccgac gagatacgcc tcacgattca
     4981 cgcttcagaa agtccgggcg atcgcggacg gcgaccattt tcaccaggtc gcggatcgac
     5041 aggactccga cgaccttctc atgttccgtg acagccaggt ggcggattcg tttcgcggac
     5101 atccgttcac tggcatcgcg gatggtgcga ttgatgtcga tgtcgagcaa cggcgaattc
     5161 atgagcgcac cgacccgcag ggtctgcgga tcgagttgat aggccagcag cttgcgcacc
     5221 aggtcgcctt cggtgatgat gcccacaagc tcatcggctt ccatgacgaa cagcgccccg
     5281 atgtgtttgt ccgccatgcg ttgcgccgct tcgagggccg tttcgtcgct gccgatggtt
     5341 tcgatggcag ttttcatcag gacactcaac gggcggtaga cattggtgag cgcctgcacc
     5401 ggcccgccgt ctgcatcgac gaagtacctc accagatcgc gtatcgagat gattccgagc
     5461 acatccgtgc cttcggtcac cacgaggtgc cgcacgtttt tactttgcat cagatgactg
     5521 gcatcgacca tcggacgagt cggcgcgatc gtgatgagcg gactgctcat gatgtccgcc
     5581 atgctggttc gagagggaac gcggcccggc gccagtacct tggcgacgag atcggtttcg
     5641 gtgacgattc ccgcaatccg gcaatcgttg tgcggacgat cgaagcattc gatcagcaca
     5701 gaaccgacgc ttcgcgtccg catttgggtg gcgacggtca cgacggaggt atcctgcggg
     5761 acgacctcca attggcggtg catatagtcg ctgacgtgct ggcccgatac ggtcgtcata
     5821 aaagctcctt ttcttcaaca tgaatgtcgc cggttggcat tgcgaacgtg cacgattgtc
     5881 tctcatcggt ctcaatcgat ccaggcatac gtcacgtccc ccggtcgggt tgcagagaga
     5941 gtcgagcgcc cattcgcccg cggacgaatt tctgaagttc gtctgccagg agcaagccga
     6001 tggccccgag cgccagcggt ccgaagatcc aggccgggag cgaggcggtg cccaagacca
     6061 gatgtccgac agggctgtag gtcatgacga gcaggatggt cagttcgacg gcgacaccca
     6121 ccaggatcaa gggattgctg aaccagccga gccggaacgc cgagatgcgg tcggagcggc
     6181 aggcaaagac atttgcgacc tgtgccgcca cgatcccggc aaaggtgacg gtcgtggcct
     6241 ccttatacaa aggcgaggtc cagtcgaggg gggctgccca gctccacccc tggctataca
     6301 aatagagaaa aaaccctccc atggcaatgc cggcttcgat taatcccaga aaggcatacg
     6361 ctctcaaaat cacatcccag ctaaggagtc gttcggttcg gggtcgcggg gggtcgtcca
     6421 tcaccccgcc ctccggcggt tctgcggcca gggccagtgc cgggaccatg tctgttccca
     6481 aatcgacggc cagaatttgc gggatcgtga gggcgagcgg catcgacgag agtccgaatc
     6541 ccagatacgg cacgatctcc gggacgttgc tggccaacac ataggttacg aacttgcgaa
     6601 tattggtata aacggcccgt ccttcttcga tggcgtggac aatggtggcg aaattgtcgt
     6661 cgagcagaat catggaggcc gtctctttgg ccacgtccgt tccggcgata cccatggcga
     6721 tgccgatgtc ggcagttttc aacgccggcg cgtcattgac cccgtcgccc gtcacggcga
     6781 ccacttcgcg catttccttg agcgccgaga cgatgcgcat tttgtgtcgg ggcgccatgc
     6841 gggcaaagat cggatccggt tcgccgggag cggtcggggt gagcagttgg cgcagctgat
     6901 cgtcgctgaa tgtgtcgatg tgtgcgcctt cgatcaccgg gacgaaatgg cccggttccg
     6961 tgggtatcgt ttccggagcc agcccgatct tgcgtgcgac ggccaatgcc gtcaggggat
     7021 ggtctcccgt aatcatcatg acgcggaccc cggcccgccg gcattttttg atcgcgtccg
     7081 gtacttcacg gtgtggcggg tccatcatgg cgacgagtcc gagaaacgtc agtccctgtt
     7141 ctaccgtgtc ggcctctaac tggtccacgc cgcgttcgac ttctcgcatg gccacggcca
     7201 gcacccggta cgcctgctga gcgaaggcct gactctgggc cagcactttc ttgcggccgt
     7261 cgacggtgag ctcggcgggt gtcgacgatc cctgctgcct ggtgcacaac ggcaagaccg
     7321 attccggcgc gcctttggtg aaggcgagca gccgaccttc gctccagtgc agcgtcgtca
     7381 tccgtttgcg atccgcatcg aagggcattt cgcccattcg ccggagcggc gggcggtgga
     7441 ggagtccgtg gtccgcggca aactccagga gcgccgtctc agtgggatcg ccggtggtgc
     7501 ggctacgccc gtccgggtgg cgcgtgcgct tggcgttgtt gcaatgaacg atggcgtcga
     7561 acaggggacg ccagcgttcc gattcgggag cggtgatgag gcgattcctg gtgaacaaac
     7621 agccttctcg cgattcgacg acgaggtcgt ccacgtagag cctgtcgagc cgcatccggt
     7681 tttccgtcag tgtcccggtc ttgtcggtgc agatcacggt ggtgcacccc agtgtctcaa
     7741 cggacgcgag gtgtttgatg agcgctttgc gcgcggccat gcgttggctg cccatggcga
     7801 gggccagggt gacggtgggc agaagccctt cgggcacatt ggcgacgatg atgccgatgc
     7861 cgaaaatcgt gctcgcccag aatcccaatc ccatcccgac gccgatggtg aagaagacca
     7921 ggcccatcag cagtgagagc ccggcgacga catgcgtcac cttgacgatt tcctgttgga
     7981 gcgggctcaa cccggtttgt accgttgtcg tcagatgcgc gattttgccg aattcggtgt
     8041 tgaggccggt ggcaaagacc acgccctggc catggccgga gaggaccgta gttccggcga
     8101 aggccagatt cgcaatgtcg agcggatggc cgtcggtgat gggatcggcc gaccgtcgtt
     8161 gcggtttcga ttcgccggtg agagaggagt tatcgacgcg catgccgacg gctgcggtga
     8221 gtctcgcatc ggcaggaatc tgctcaccct cttccagcac gagcacatcg cccggtacca
     8281 gttcgctgcg ggcaatctgt tgctgttgtc cgtcgcgaag gacccaggct tgggccggca
     8341 gcatgctgcg taaagcctga accgcgcgtt cggccttata ctcttgcagg aaggcgaaaa
     8401 tggcattgat gagaatcacg ccgaggatgg cccaccctag cgtcgccatg ccttcgccgg
     8461 agtggagggc atcggcaacg aacgccaggc ctgccgccac ccacaggagc agcgccagga
     8521 aatgcgtgaa ctgacgcgca aagcggctga tgagcggcat cccgcgcaat gcctgcagac
     8581 tattcggccc gtaccggagg gttcgtcgcc ggacgtcgtc cgacgagagg ccctgcgggg
     8641 tggtggcgag cgcgcgatac acctggctcg gagccaggcg gcagacttct agcgcagtca
     8701 gttcaggcac gccatcctcg tacgatgagc accgaacagg gggcatactt cagcagcgtt
     8761 tccgaaacgg tgcccaactg aagccgttcg gaccccgtca agcctcgtga gcctgccacg
     8821 agcagatcca ccttgccgga ggtggcctgt tggatgatcg tgtcggtcac atgatcgacc
     8881 ttcacctgcg tcgtgacggc gcaaccttcg gccaggaacc gatcacgtaa gttctctacg
     8941 acctgttcgg cggcc
//
LOCUS       DAORRQ010000106         8633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_341_length_8633_cov_37.703661, whole genome shotgun sequence.
ACCESSION   DAORRQ010000106 DAORRQ010000000
VERSION     DAORRQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_341_length_8633_cov_37.703661"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK224_19410"
     CDS             <1..468
                     /locus_tag="PK224_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HRB17973.1"
                     /translation="MMYVDMKMYLPDDILVKVDRASMAVGLEARVPFLDHRLVEFAWR
                     LPIAMKFHEGHGKWILRRVLDRYVPATLLDRPKMGFGVPIDGWLRTPLKEWAEALLDE
                     KRLRDQGFLNPDAIRQKWNEHLSGEFNWKGQIWVVLMFQAWLEHMQQDLPSVE"
     gene            501..1412
                     /locus_tag="PK224_19415"
     CDS             501..1412
                     /locus_tag="PK224_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17974.1"
                     /translation="MLPTAQELFLNSSQGLSDRSASQDLLEEKFFYSIELSNGTHKRT
                     WAGRLDDVDQVLLAVFRKFDVTPKTFLDVAVSSGVSTVSWCDALRQAGLRPSMTATDL
                     TMTAYLIRLHDWLHVLVDKEGFPLQYDVCGYAWRPRCRIRLYPLGNGALTVLWDVLYR
                     KTAGKYDLLARLKSLGLTTPAVGDPLIKAEIKLVTRRLRDNPDIELLDDDILAPTPAH
                     LQRRFEVIRAANILNRGYFSKEQLRTAVTHLRDRLVGDGSFFLVVRTDETATNNGTLF
                     LLNADGTFRVVDRIGAGSEIEDIVLSL"
     gene            1467..2474
                     /locus_tag="PK224_19420"
     CDS             1467..2474
                     /locus_tag="PK224_19420"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRB17975.1"
                     /translation="MNGQRGTMVVSLDLELCWGRFDKVPVPMLEADASEERIQIKRLL
                     ALLDRYEIPATWAIVGHLMLAGCSRHAGAAHTDVMPRPDYSWFPKDWYVHDPCTSAIQ
                     SPGWYAPDILEWIRATRVRHEIASHSFAHIYYGDPECSATAARADLTAAMAAASQHDV
                     PLRSFVFPRNQVGHLDVLRAQGIRAYRGADPTRFRRTKGILYKTLSFLDQLLGLPPKA
                     VRAAEVMPGLWNIPGNHFYMARNGLRKMIPMASRVWKGKQGIRQAIQTGGVYHLWFHP
                     FNLNADADAMLSGLEQLFAYARRLREQGVLDIFTMDDYRARLTACASETVGTASSGVL
                     A"
     gene            2524..3762
                     /locus_tag="PK224_19425"
     CDS             2524..3762
                     /locus_tag="PK224_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB17976.1"
                     /translation="MASIKLVHVTTVPQSLFFLRGQVGYMKERGFDIHGLSSPGEFLS
                     RFAEQEHVAVHPVEMPRRITPMRDLAAVVRIWTVLRQVRPQIVHAHTPKGGVLGMVSA
                     WLARTPVRIYHMRGLPFTTASGWKRTLLIWSERISCRLADQVFCVSSSVRQVALENGL
                     CPPDKIAVLARGSGNGVDAVWRFNGADLSPALRDETRRRYEIPCDALVLGFIGRIVRS
                     KGIIELAGAWKRLRAEFPNLHLLLVGPYEAQDPISPEVDEELRRDARVHLIGEEWNTP
                     PLYAAMDVLALPTYREGFPNVLLEAAGMKVPVVATRVPGCTDAVQDGVTGLLVSPFDP
                     EPLAAAVKEYLLDPELRRRHGTAARDWVLREFRPQGIWQAIHQEYVRCLRGKGLLPTD
                     DHGREKRGNAESDHSRAQCS"
     gene            3770..4042
                     /locus_tag="PK224_19430"
     CDS             3770..4042
                     /locus_tag="PK224_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17977.1"
                     /translation="MQTQSRVQPQQWVVKTRQGSVVGHVKSLTICHCSKQIVSALLVL
                     RNSGQQVQIPWNRFDMVEEQLVLRVPVGRLTRELQSCRETHGQATL"
     gene            4023..5285
                     /locus_tag="PK224_19435"
     CDS             4023..5285
                     /locus_tag="PK224_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17978.1"
                     /translation="MARQLSDMESREAINRESSATRSGRRISRRALVTGLLLTVLVCS
                     LLLEVCSYVYLRVAEGYDGEHLMMHQFDDYKNIHPTPGYQDTRGVIHNAQGFRRAEDV
                     AMTKPSGTYRIFLMGGSTAYGLGSLSPQGHQKYPVLKNSETIDHYLEKFLHRTVPSRR
                     IEVINAAIPSHSSHHHLIYLNQTILKYHPDMIVFIDGYNDYYPWEQNYDQFRDYPYRQ
                     WSHLYLDEPSLKAWAGYSGFWLYRKSHFVYLAGKKLRPLWWAIQNLRPQPRRELNVDD
                     ALKNLEMNAEVNFLRMVERDALILKHEGVVPVFTLQPDLLFEQRHSLTEFEQRLSAEV
                     DQAQPINYRQFKNRARPMVTARLQQVTAARGASFIDMTDILGDIKEDAFTDDCHLTPI
                     ANKAVADYIGHRIAPLISVPAEGKEDRK"
     gene            5282..5929
                     /locus_tag="PK224_19440"
     CDS             5282..5929
                     /locus_tag="PK224_19440"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRB17979.1"
                     /translation="MKKGRSHHTDGGVPLRPGKRLLDLTVILLALPIALPFLGMIALI
                     TWAIQGSPVLFRQQRPGLRGKPFTIYKFRTMRALPPDATATAKSDGDRLTRFGRFLRS
                     TSLDELPELINVIKGDMSLVGPRPLLMQYLDRYTPEQQRRHDVLPGITGWAQVNGRNA
                     LTWEEKFDLDLWYVANHSLWVDLKILAMTAWKVIRREGIQHPGQVIMEEFKGYRN"
     gene            6121..7143
                     /locus_tag="PK224_19445"
     CDS             6121..7143
                     /locus_tag="PK224_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRB17980.1"
                     /translation="MKAHTFGDAVEVFATSPQSAESTTEAYLKEVIEIAQWSEQEGCT
                     GTLVYTDNRLADPWLVAQIILQNTKTLCPLVAVQPLYMHPYAVAKMVSTLAFLHGRRV
                     YLNMVAGGFAYDLKAFKDTTPHDKRYDRLREYTHIIQALLTGAPVTYQGEYYAVDNLR
                     LTPALPPDLFPGIFLSGSSEAGLDCCRALGATAIKYPKAPNEEMNALDGLEKAGIRIG
                     VIARPEEDVAWNVAYKRFPEDRKGQLAHQLTMKVSDSLWHKQLSDLAESTAVDRNPYW
                     LVPFQNYKTFCPYLVGGYETVAREIARYIGAGYRSFILDIPPDKEELRHTGVAFQQAW
                     AMAQKG"
     gene            7199..>8633
                     /locus_tag="PK224_19450"
     CDS             7199..>8633
                     /locus_tag="PK224_19450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRB17981.1"
                     /translation="MRRVGSRPALYVDGKVHSYEELGRLSGRIAATIRNTSPPSMPLV
                     ALLAHRSLTAYAGVLGILGAEKGYVPLNPKLPAERLLRILTLAGPDVLIVGKEARKHL
                     ELLLPKLPQRTVMICPDMEDLDGLSASFPQHQFVPGSAMAEGALIAADPREGDDPIAY
                     LLFTSGSTGDPKGVPVRQSNLAAYVRYIADRYAVNEEDRFSQMFEMSFDLSVHDMFVC
                     WEGGACLYAMPDHTVMAPAKFIRDHAITMWFSVPSVIGVMEKLGMLKAGLFPTLRTSL
                     FCGEPLLGRHAQVWQEAAPHSLVENLYGPTEATIAITHYRWNPATSPDICKSGITPIG
                     RAFSGQKTCVIDSTFHSVAAGDSGELCLSGSQVTQGYWNNPEQTKKQFVTLPGAGDDI
                     WYRTGDLVMEDQDGCLQYLGRIDHQVKIRGHRVELPEVEFTLREASGSQQVAALAWPV
                     KEGLAEGIVAFIAGDAAQDVGRILQHCR"
BASE COUNT         1754 a         2431 c         2594 g         1854 t
ORIGIN      
        1 atgatgtacg tcgatatgaa aatgtatttg cccgacgaca ttttggtcaa agtggaccga
       61 gccagtatgg cggtcggtct ggaggcgcga gtgccgttct tggatcatcg tcttgttgag
      121 tttgcctggc gactgcccat tgccatgaaa tttcatgagg gacatggcaa atggatcttg
      181 cggagagtct tggatcgata tgttcctgcg accctcctgg atcggccgaa gatgggattc
      241 ggcgtcccga tcgacgggtg gctacgcacc cccttgaagg agtgggccga agctcttctc
      301 gacgaaaaac ggttacggga tcagggattt ctcaatccgg atgccattag gcagaaatgg
      361 aacgaacatc tttccggcga gtttaattgg aaaggccaga tttgggtggt gctcatgttt
      421 caggcatggt tggagcacat gcagcaggat ctgccgtcgg tcgagtagga accatccgcg
      481 ttatacagag gattgtttac atgcttccca ccgcacaaga actctttctc aacagcagtc
      541 agggcttgtc cgaccgttcc gcttcacagg atctcctgga agaaaagttc ttctattcca
      601 tcgaactgag caacggcact cacaaacgaa cttgggccgg tcggctggac gatgtcgatc
      661 aagtcttgct cgctgtcttt cggaaattcg atgtgacccc gaagaccttt ctcgatgtcg
      721 ctgtttcgtc cggggtgtcg accgtttcat ggtgtgatgc attgagacag gcagggctcc
      781 gaccgagcat gaccgctacc gatttgacga tgaccgccta tctgatacgc cttcacgatt
      841 ggctgcacgt actcgtagac aaagagggct tccctcttca gtacgatgtg tgtgggtatg
      901 cgtggcggcc tcgctgtcgc atccgattgt atcccttggg aaacggggcg ttgacggttc
      961 tgtgggatgt tctataccgc aagacagctg ggaagtatga tctcctcgcc cggctgaagt
     1021 cgttggggct caccacacca gcggtaggag accccctgat caaggcggaa atcaagctgg
     1081 tgacacgcag gttgcgagac aatccggaca ttgagctcct ggacgatgac attctcgcgc
     1141 ccactcctgc tcacttgcag cggcgcttcg aggtgatccg ggccgcgaat attctcaatc
     1201 gcggctattt ctccaaggag cagttgcgca ccgcagtaac ccatctgcgc gatcgcctcg
     1261 tgggagatgg gtcgttcttc ttggtcgtgc gcacggatga aacggccaca aataatggaa
     1321 cgctgtttct gctcaatgca gacggaacat tcagggtcgt cgaccgtatc ggtgcggggt
     1381 ctgagattga agatattgtt ttgagcctct gaggcggagt tcacgtttcc ggggatgaag
     1441 cgtcggcgta acgaaggata ccttgcatga acggacagcg cggtaccatg gtagtcagtc
     1501 tcgatctcga actctgctgg gggcggttcg acaaagtgcc ggtgccgatg ctcgaggcag
     1561 atgcttcaga ggaacggatc caaatcaaac ggctgctggc gctgctcgat cgttatgaaa
     1621 tcccggcgac ctgggcgatc gtgggccatt tgatgctggc cggctgctcg cgccatgccg
     1681 gcgcggccca cacggacgtg atgcctcgtc cggactattc ctggtttccg aaggactggt
     1741 acgtccacga cccctgtacg agcgcgattc aatcacccgg ttggtatgct cccgatattc
     1801 tggaatggat cagggcgacg agggtgcggc atgaaattgc gtcgcattcg ttcgcccaca
     1861 tttattacgg tgaccctgag tgcagcgcca cggcagccag ggccgatctc acagccgcca
     1921 tggcggctgc ctcccagcac gatgtcccgc tgaggagttt cgtgttcccg cgcaatcagg
     1981 tcggacatct tgacgtgttg cgcgcacagg gtatccgtgc ctatcgcggg gcggacccca
     2041 ctcggtttcg tcggaccaaa gggatcctct acaagacgtt gagctttctt gaccaactgc
     2101 tggggttgcc tcccaaggct gtgcgtgccg cagaggtgat gccgggactc tggaacattc
     2161 caggcaatca cttctacatg gcgcgcaacg gtcttcgtaa gatgattcct atggccagcc
     2221 gtgtctggaa aggcaagcag ggaattcggc aggccattca gaccggcggg gtctatcacc
     2281 tgtggtttca cccgttcaat ctgaatgcgg acgccgatgc catgttatcc ggcctggagc
     2341 aactctttgc ctatgcccgc aggttgaggg agcagggtgt cctggacatc ttcaccatgg
     2401 atgactatcg cgctcgcttg accgcgtgcg cgagcgaaac cgtgggcaca gcgtcgagtg
     2461 gcgttctcgc atagagcgca cagtctcttt cttcatccac acccctgagt cagttggttt
     2521 gaaatggcgt ccatcaagct cgttcacgtc accactgtgc cgcagtcgct gttctttctc
     2581 aggggccagg tcggctatat gaaggagcgt gggttcgaca ttcacgggct ttcgtcgccg
     2641 ggagagtttc tctctcgttt cgccgaacag gagcatgtgg ccgtgcatcc ggtcgagatg
     2701 ccccgacgca tcactcccat gcgcgacctt gcggcggtcg tgagaatttg gacagtgttg
     2761 cgacaggtcc gccctcagat cgtccatgcc cacacgccga agggaggcgt actggggatg
     2821 gtcagcgcct ggctcgcccg caccccggtg cggatctacc acatgcgcgg attgcccttc
     2881 actaccgcct ccggctggaa acggacgctc ctcatctgga gcgagcggat ctcctgcagg
     2941 cttgccgacc aggtgttttg tgtcagttca tcggtccgtc aggtcgcgtt ggagaacggg
     3001 ctctgcccac ccgacaaaat tgcggtgctt gcgcgaggca gcggcaatgg tgtcgatgcg
     3061 gtctggcggt ttaacggggc ggatctgtct cccgctctgc gcgatgagac caggcgacgc
     3121 tacgagatcc cctgtgacgc gctggtgctg ggattcatcg gccgcattgt ccgcagcaag
     3181 gggatcatcg aattggccgg ggcatggaaa cgattgcggg ctgagtttcc gaatctgcat
     3241 ctcttgcttg tgggaccgta cgaagcgcag gaccctattt caccggaggt ggacgaggag
     3301 ctccggcgcg atgcgcgcgt gcatctgatc ggggaggagt ggaacactcc accgctgtat
     3361 gccgcgatgg atgtgctggc gctgccgacc tatcgagaag gatttccgaa tgtcctgctg
     3421 gaagccgccg gaatgaaggt gccggtcgtt gcgactcgag ttcccggctg cacggacgcg
     3481 gtgcaggacg gcgtcaccgg cttgttggtg tccccgtttg acccagagcc gctggccgcg
     3541 gcggtcaaag agtatctgct cgacccggag cttcgtcgcc gtcatggaac ggcggcgcgt
     3601 gactgggtgc tgcgtgagtt tcgcccacaa ggcatctggc aggccattca tcaagaatac
     3661 gtgcgttgtt tgcgcggcaa aggccttcta ccgacggacg atcatgggcg cgagaagcgg
     3721 ggtaatgcag agtccgacca ttcccgtgcc caatgctcgt gatctcatca tgcagacgca
     3781 atcacgcgta cagccgcagc agtgggtggt caagactcgt caagggagcg tcgtgggaca
     3841 cgtcaagagc ctgacgatct gtcattgctc gaagcagata gtatcggccc tgcttgttct
     3901 gcgtaacagc ggacagcagg tgcagattcc ttggaatcga ttcgatatgg tcgaggaaca
     3961 attggtgctt cgtgttccgg ttggtcggct tacccgcgag cttcaatcgt gtcgggaaac
     4021 ccatggccag gcaactctct gatatggaat ctcgtgaagc gataaatcgg gaatccagtg
     4081 cgactcgatc ggggcgtcgg atctcccgcc gcgcgcttgt caccggactt ctgctgacag
     4141 tgcttgtgtg ctcgctcctc ctggaggtgt gttcctatgt ctacttgcgt gtggcagagg
     4201 gatacgacgg ggagcatctc atgatgcatc agttcgacga ttacaagaac attcacccga
     4261 cgcccggcta tcaagatacg agaggtgtca ttcacaacgc ccagggtttt cgccgggctg
     4321 aagacgtagc catgacgaag ccgtcaggca cctataggat ttttctcatg ggtggatcca
     4381 cggcctatgg gttggggtcg ttgtctccgc agggccatca gaaatatcca gtgctcaaga
     4441 attcggagac catcgatcac tacttggaga aattcctgca ccgcacggta ccgtcacgtc
     4501 ggatcgaggt gatcaatgcg gccattccaa gccattccag tcaccaccat ctgatctatc
     4561 tgaatcagac cattttaaag taccacccgg acatgatcgt gtttatcgac gggtataacg
     4621 attattaccc ctgggaacag aactatgatc agtttcgtga ctatccgtac cgccaatggt
     4681 cgcaccttta cctcgatgaa ccgtcattga aggcttgggc cgggtattcc ggtttctggc
     4741 tctaccgcaa gagtcatttt gtttatctgg cggggaagaa attacgtcca ctgtggtggg
     4801 ccattcagaa cctccgtccg cagccccgtc gcgagttgaa tgtggatgat gcgctgaaga
     4861 acctcgaaat gaacgccgag gtcaactttc ttcgcatggt cgagcgggat gcattgattc
     4921 tcaagcatga gggggtcgtc ccggtcttca cattgcagcc ggatctcctg ttcgagcagc
     4981 gacacagtct gacggaattc gaacagcgtc tgtcggcgga ggtcgatcag gctcagccga
     5041 tcaactatcg gcaattcaag aatcgcgcac gcccgatggt caccgccagg ctccagcagg
     5101 tgacggccgc gaggggtgcg tccttcatcg atatgacgga tattctcggc gacatcaagg
     5161 aagatgcgtt cacggatgat tgccacctca ctccgattgc caacaaggca gtggcagact
     5221 acatcggcca tcgcattgcg ccccttatct ccgtgccggc agaggggaag gaagatcgca
     5281 agtgaagaag gggcgatctc atcatacaga tggcggcgtc ccactgcgac caggcaaacg
     5341 gctgctggac ctgacggtga tcctgttggc gcttccgatc gcgcttccgt tcctggggat
     5401 gatcgcgctg atcacgtggg ccattcaagg ctcacccgtg ctgtttcgac agcaacgccc
     5461 cggtctccgg ggcaagccgt ttaccattta caagtttaga accatgcgtg cgcttccccc
     5521 cgacgccacg gcaaccgcga agtccgatgg agaccggctg acgcgattcg ggaggttctt
     5581 gcgaagcacg agtctcgacg aattgccgga gctgatcaac gtcatcaagg gcgacatgag
     5641 cctggtaggg ccgcggccct tgctgatgca gtatcttgat cgctacacac ccgaacaaca
     5701 gcgccggcat gatgtcttgc cgggtattac gggctgggcg caagtgaacg gtcgaaacgc
     5761 gctgacctgg gaagaaaagt tcgacctcga cctctggtac gtggccaacc attccctctg
     5821 ggtcgatctt aagatcctcg ccatgacggc ctggaaggtg atccggcgcg aggggattca
     5881 acatcctggc caggtgatca tggaagagtt caagggctac cggaactgag tctgctccac
     5941 ccccgacccg acgagcctct caacagacgt ccttcaccgc ctcgcaccat tccgaaggac
     6001 gtcgccatga catgggatcc acgctgcggc ctgcgtcgcc gactgcccga tccatcgcag
     6061 ttctgtccac cgtaattctg cagtgcgcca acagatccat ttcggcaaag gaaggtcgct
     6121 atgaaagctc acacgttcgg tgatgcagtc gaagtgttcg ctaccagtcc ccaatcggca
     6181 gaaagcacca cggaagcgta tttgaaagag gtcattgaga tcgctcaatg gagcgagcag
     6241 gagggctgca ccggcacgct cgtctatacg gataaccggc tggccgatcc ctggctggtc
     6301 gcacagatca ttctccagaa cacgaagaca ttgtgcccgc tggtggcggt acagccgctc
     6361 tacatgcatc cctatgccgt agccaaaatg gtcagtactc tggcctttct ccacgggcga
     6421 cgcgtgtacc tgaacatggt cgccggcggg ttcgcctacg acctcaaggc gtttaaggac
     6481 acgacgccgc acgataaacg gtatgaccga ttgcgtgaat acacgcacat catccaggcc
     6541 ctgctgacag gggcaccggt gacctatcag ggagagtatt acgcggtgga caatcttcgg
     6601 ttgacgccgg cgttgccgcc cgatctgttc cctggaattt tcttgtccgg ttcatccgag
     6661 gcggggctcg actgttgccg cgctctgggc gccaccgcaa tcaaatatcc taaggcaccc
     6721 aacgaagaaa tgaacgctct cgatggactg gagaaggccg ggatcaggat cggcgtcatc
     6781 gcacgacctg aagaagacgt agcctggaac gtggcctaca aacgatttcc ggaagaccgg
     6841 aaagggcagt tggcccacca attgacgatg aaagtgtcgg attccctctg gcacaagcag
     6901 ttgtcagacc ttgccgagag cacagcggtg gatcgaaatc cctactggct cgtgcctttc
     6961 cagaactaca aaacattctg tccctacctc gtgggcgggt atgagaccgt ggcacgggag
     7021 atcgcccgct atatcggcgc ggggtatcgg agtttcattc tcgatattcc accagacaag
     7081 gaagaactgc ggcacacggg cgttgcgttt caacaggcct gggccatggc tcaaaagggg
     7141 tgaccatatg gggatacagc aaccgataag cggccccttg agggggttcg tggaatccat
     7201 gcgcagggtc ggatcaaggc cggccctgta cgtcgacggc aaggttcact cctatgagga
     7261 gttgggccgc ctgtccggga gaatcgcggc cacgattcga aacacgtctc cgccctccat
     7321 gccgctggtc gccttgctgg cccatcggag cctcacggct tacgcggggg tactgggcat
     7381 cctcggagcg gaaaagggct acgtaccgtt gaatcccaag ctgccggctg aacggttgtt
     7441 gcggattctc accttggccg gccccgacgt cctgatcgtt gggaaggagg cccggaaaca
     7501 tctggaactg ctgcttccca aacttccgca gcgaaccgtc atgatctgcc ccgatatgga
     7561 ggatctcgac gggctgagcg cctcatttcc gcaacatcag ttcgtacccg gcagcgccat
     7621 ggcagaggga gcacttatcg ctgccgatcc ccgggaaggc gatgatccga tcgcctatct
     7681 gttatttacg tcgggcagta ccggtgaccc aaaaggcgtg ccggttcggc aaagcaatct
     7741 cgcggcttat gtccggtata tcgccgaccg gtatgcggtg aacgaggaag accgcttttc
     7801 acaaatgttc gagatgagct tcgatttgag cgtgcacgac atgttcgtct gctgggaagg
     7861 aggcgcctgt ctgtatgcga tgccggacca taccgtgatg gcgcccgcca agttcatccg
     7921 cgaccatgcc attaccatgt ggttctcggt tccgtctgtc atcggggtga tggagaaact
     7981 cggcatgctg aaggcgggac tgtttcccac gttgcggacg agcctgtttt gcggagaacc
     8041 gttgctcggt cggcatgccc aggtctggca ggaggccgca ccccattcgt tggtcgagaa
     8101 tctctatggt ccgacggaag cgaccatcgc gattactcat tatcgctgga atcccgccac
     8161 ctcgccggat atctgcaagt cggggatcac gccgatcggc cgcgcgttca gcggccagaa
     8221 gacctgcgtc atcgactcta cgttccattc ggtcgccgcg ggagacagcg gggagttgtg
     8281 tctctccggc tcccaggtga cgcaggggta ttggaataac cccgagcaga ccaagaaaca
     8341 gttcgtcacc ttgcccggag caggcgacga catctggtac cggacaggcg atctggtgat
     8401 ggaggatcaa gacggctgtc tgcagtatct cggacgaatc gatcatcaag tgaaaatccg
     8461 agggcatcgg gttgaattgc ctgaagtcga gttcaccttg cgggaagcat cgggatccca
     8521 gcaagtcgcg gcgctggcct ggccggtgaa ggaaggcctg gccgagggga tcgtcgcgtt
     8581 tatcgccgga gacgccgcgc aggatgtcgg gcgtatcctg caacattgcc ggc
//
LOCUS       DAORRQ010000107         8572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_345_length_8572_cov_222.577903, whole genome shotgun sequence.
ACCESSION   DAORRQ010000107 DAORRQ010000000
VERSION     DAORRQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_345_length_8572_cov_222.577903"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1083)
                     /locus_tag="PK224_19455"
     CDS             complement(106..1083)
                     /locus_tag="PK224_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17982.1"
                     /translation="MKMRSNQIVRGIIVVGLLLVTMMVTSEAGLLGKEETPGERFRKA
                     IEKIRLSCESRKLAPGEVCGAVTNLKPADPLATEEGRFAHSIRIPNPVPEDSGYKPGM
                     TPEQYFEHLCKTEAGEFIYKTVENVEGIYMMRPRKEATDWELEHLYALEDPYGYTTGT
                     SAEQAFGFIRPDGYRYVESPLFEVKVSSWKKRYRDSSLFDPPSEKAKYARYFGFDARN
                     MKSMKMEYASSLRSRYGFTWRGISRPHDREMGIAGGELIILELASNDVVAVRRGFLKS
                     GNVKNLTGIWWLKGQVCPGELGRAEHGGDNFVLKVVKPVSSKGEHYGSR"
     gene            complement(1138..2526)
                     /locus_tag="PK224_19460"
     CDS             complement(1138..2526)
                     /locus_tag="PK224_19460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRB17983.1"
                     /translation="MKTLLQHLAVWRAAWLIEQTMPSAVVSGGSAVEFLPAVLEIQHA
                     PPSPIGRAILWTIMLVFTTAVTWSSLSHIDIVAVAPGKIIPSGHSKTVQPFETGVIAS
                     IHVQDGQTVRQGEVLIELDATQNGADRDRALNEYRAALVEAARLRALIAGQTTFDAPE
                     DADPQFLVLQQQLLRDQVAELSARVDASRHLISQRRAALDATKDNIQRLEATVPIEAE
                     RAKAYRSLLAQQYVSKMDYLQFEQQRIDRAQELAGQKSKLRQDQAALAEAEQNYHVLL
                     SEFQQSKQTELSVTETKVTSLLQEVRKAGQKTELQRLVSPIDGVVQQLAVHTVGGVVT
                     PAQPLLIVVPQDHPVEVEAQFENKDIGFVKEGQPVEIKVETFPFTLYGTIPGKVLTVS
                     DDAVPLDEKGGLVYASRVSMDRATMQVEGKRIHLSPGMAVTVEVKTGQRQVIDYLLSP
                     LLKSMQESLRER"
     gene            complement(2549..4681)
                     /locus_tag="PK224_19465"
     CDS             complement(2549..4681)
                     /locus_tag="PK224_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRB17984.1"
                     /translation="MPSPITDTGLLCLLMVARFHDLPADGGQIQHQFGQSGKVLATQE
                     LLRAAKHIGFRVGLVTARWEELATTPLPAIVQRIDGRYVVLAKVQDDKVLIHDPLEAR
                     SLVQSREMFVTNWSGRLLLCGKRAGLRAQDLAFDITWFIPAVFKYRRLLGEVLLASFF
                     LQLFALLTPLFTQVVIDKVLVHKGFTTLHVMAIGMLALALFDGLLGGLRTYLFAHTTN
                     RIDVSLGAQLFRHLLALPLAYFEARRVGDTVARVRELEHIRQFLTSSSVTVVLDVVFI
                     AVFIAVMWLYSSMLTLVVMASLPLYAILSIAITPTIRTRLNEKFNRGAENQSFLVEAV
                     GGIQTVKALAVEPPLQRRWDEQLAGYVQASFRATSLITIAGQLATFIQKTTTIAVMWV
                     GAYQVIDGALSIGELIAFNMLAGQVTGPLLRMVNLWQEFQQVGISIQRLGDVLNTRPE
                     PAYSPARTTLPRIAGQITFDDVTFRYRPDGRPVLQQVSFSLQPGQVIGMVGRSGSGKS
                     TIAKLMQRLYVPERGRVLVDGVDLAQVDPAWLRRQVGVVLQENFLFNRSVRDNIALTD
                     PGLSMDRVMHAAKLAGAHEFILELPDGYDTIVGEHGCALSGGQRQRIAIARALVANPR
                     ILIFDEATSALDYESEAIIQQNMAQICKGRTVIVIAHRLSTVRPAHRIYVLDKGQIFE
                     DGTHDDLVKRNGMYARLHRYQEGRSAAA"
     gene            complement(4741..5076)
                     /locus_tag="PK224_19470"
     CDS             complement(4741..5076)
                     /locus_tag="PK224_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17985.1"
                     /translation="MAESHHPMQRGDKSVAVNVRLKPVSPSDQPILVNYTHVGLSDGL
                     AYVDFGFLEPALLSAVATRAKQGEVTPKQVEGISAARVALPLDSVVRLYQQLQRVLVS
                     LQPAKTKQS"
     gene            5628..5867
                     /locus_tag="PK224_19475"
     CDS             5628..5867
                     /locus_tag="PK224_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17986.1"
                     /translation="MLRFESTELLPGRHVGTVGLTKDGCVMVWKSTPELTHGRIGFSL
                     TYIQKLEHQDGEKWVEVPLTPMVHHEPNKCQEPTG"
     gene            6032..6637
                     /locus_tag="PK224_19480"
     CDS             6032..6637
                     /locus_tag="PK224_19480"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRB17987.1"
                     /translation="MYTPINKLLMQGVVDSWIRLSASLAVWAFCLTVTACAGVEHKDT
                     LPTVASVDLARYAGTWYEIARLPMWFQRHCVDSKAIYTSRPDGRIGVHNECLTDSGGV
                     DQAEGVATVIDPKTNARLTVVFDNFFARLFGASRDGNYWILALDPEYRTALVGTPDRR
                     FLWILSRAPQLEEVTYQRLVEQAKRLGFPTADLIRTRRPAS"
     gene            complement(6712..7473)
                     /locus_tag="PK224_19485"
     CDS             complement(6712..7473)
                     /locus_tag="PK224_19485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB17988.1"
                     /translation="MTIMPQRILIADDEETFRESTAEILAEAGYACSAARDAAEAESL
                     LENGYDLLLADVRMPGNAHLEFLENVSRRHPELPVVVITGYPTVGTAVESFRLSIVDY
                     LIKPVGVDDLKRAVERGLRRRTLARAVKDAMTETAKVAGIFDQLGQSMREFGRGEPQV
                     EWSAHEYMAQAMGHIAQLSALVAQTLEASRGSRPQAPTDVCALMQCPRLDRYEAAIQD
                     AVEVMERTRSSFKSKEIGALRQRLETLLREGRRQS"
     gene            complement(7439..>8572)
                     /locus_tag="PK224_19490"
     CDS             complement(7439..>8572)
                     /locus_tag="PK224_19490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB17989.1"
                     /translation="GLTADQLMGRTPVDPGWQAIHEDGSHFAPDQRPPMVTLRTGRPC
                     SNVIMGLSRPTGEQRWISINTQAILRASDARPYAVVGSFHDITERRRAEQALLEVQSQ
                     LEQRVRERTARIQQLESQRSQAEKLAALGHLAADIAHEVNNPLAGITNAFHLVKQGIG
                     SEHRHYRFVDLIDREIQRLTAIVKKMYTLYQGLPATERQATDIAELLQDVATLLEHKM
                     AVKRLRLHTEVDTPLQVVNVSRSDLFQVLLNLVQNAIDASPPDSEVVLNVSEREGLLR
                     WSVTDCGTGIAPDVLPRMFEPFFTTKPGAGWQGLGLGLSVSRGLVQAMGGRIEVQTEL
                     QRGSTFTVVYPLEPPDMSGERAAGVNADKEQKDDDYATTHSHC"
BASE COUNT         1881 a         2606 c         2230 g         1855 t
ORIGIN      
        1 agttgaagtt ggccgatcac ccgttgagga ctcgatagat caatcccgtc aaggtaactc
       61 tcagcggcaa tctgctgtag agcgaaacgg agataggtac caatttcacc ttgagccata
      121 atgctcccct ttagaagaca caggtttaac tactttcaga acaaagttat cgccaccatg
      181 ctcagcacgg cctaattcgc cagggcaaac ttgccccttt agccaccaaa tacctgtcag
      241 gttcttcaca ttaccgctct tgaggaaccc ccgtctgact gccaccacgt cgttgcttgc
      301 aagctcgagg atgattaatt ctcctccagc aatccccatt tcccgatcat gcggccgaga
      361 gatacctcgc catgtgaatc cgtatcgact cctcaaggag ctggcatatt ccattttcat
      421 gctcttcatg tttcgagcat caaatccaaa ataacgcgca tatttcgctt tttcggacgg
      481 agggtcaaac agtgaggaat ctctgtatcg cttcttccag gacgacacct tcacttcaaa
      541 taatggactc tccacatagc gatagccatc tggacgaata aatccaaaag cctgttcagc
      601 tgaagtccca gttgtgtagc catacggatc ctccaacgca tagaggtgtt ccaattccca
      661 atctgtggct tctttcctag gccgcatcat gtagatccct tccacattct ccactgtctt
      721 gtagatgaac tcccccgctt cggtcttgca caggtgttca aaatactgct ccggggtcat
      781 tcccggcttg tacccgctgt cctccggtac cggattaggg atcctgattg agtgcgcgaa
      841 ccgaccctcc tctgtcgcca agggatcagc cggtttgagg ttagtcaccg ctccacacac
      901 ttcccccgga gccagcttcc ggctttcaca acttagcctg attttctcaa tggccttccg
      961 aaatcgctct ccaggcgtct cttctttccc caagagcccc gcctcgctgg ttaccatcat
     1021 cgtgacaagc aacaacccta caacgataat gcccctgaca atttgattag acctcatctt
     1081 catgaccaac cctcttcctg aaggtttgtt gcgcgacaca gttccttttc agatttctta
     1141 acgctccctc aaactctcct gcatagactt caataaggga ctcagcagat agtcgatcac
     1201 ctgtcgctga ccggtcttga cctccacggt aaccgccatc ccaggcgaca gatggatgcg
     1261 ctttccttcc acctgcatcg tggctcgatc catacttacc cggctcgcat aaaccaggcc
     1321 gcctttctcg tctaacggta ccgcatcgtc cgagacggtc aacacctttc ctggaatcgt
     1381 cccatataag gtgaacggaa acgtctcgac tttgatttca actggctgcc cttccttcac
     1441 aaagccgatg tccttgttct cgaactgcgc ttcaacttcc acagggtggt cttgaggcac
     1501 gacgatgagt aatggttgcg caggcgtgac gacaccaccc accgtatgca ccgccagctg
     1561 ctgcaccacc ccgtcgatcg gcgacaccag cctctgcaac tcggttttct gtccagcctt
     1621 gcgcacttct tgaagcagtg acgtaacctt cgtttcggtg acggagagct ccgtctgctt
     1681 gctctgctga aactctgaaa gaaggacatg atagttctgc tcggcctcgg ccagcgcagc
     1741 ctgatcttgc cgcaacttac tcttttgccc agctaattcc tgcgccctat caatgcgttg
     1801 ctgttcgaat tggagataat ccatcttgga cacgtactgt tgcgctaaca gagaacgata
     1861 ggccttagct cgttcagcct caatcggaac agttgcctct agccgctgga tattatcctt
     1921 cgtcgcatcc aacgccgccc ggcgttgact aatgaggtgc cgggatgcat caacccgggc
     1981 ggaaagctcc gcgacttgat cccgcaagag ttgttgttga agcactagaa attggggatc
     2041 ggcgtcttcc ggagcatcga aagtggtttg acccgcaatc aacgcgcgca aacgagcagc
     2101 ctcgaccagc gcggctcgat attcgttgag cgcccgatcc cgatcagcgc cgttttgcgt
     2161 cgcatccagc tcgatcagca cctctccctg acgaacggtt tgaccgtctt gcacgtgaat
     2221 ggaagcaatg acaccggttt cgaatggctg gacggttttg gaatgaccac tggggataat
     2281 cttgccaggc gccaccgcga cgatatcaat gtgactgagc gaggaccagg tcaccgccgt
     2341 cgtgaacacc agcatgatgg tccagagaat ggcacgaccg atcggcgaag gcggcgcatg
     2401 ttgaatttca agcacagcag gaagaaactc caccgcactc ccgccagaaa caaccgccga
     2461 gggcatggtc tgttcaatta gccatgccgc acgccacacc gccagatgtt gcaataacgt
     2521 cttcatcctc tcttcctcat ctgcctcgtt acgctgctgc ggatcgccct tcctgatatc
     2581 gatgaagtcg cgcatacatc ccgttgcgct tgaccagatc atcgtgcgtc ccgtcctcga
     2641 aaatctgccc cttgtcgagg acgtaaatcc ggtgggcagg gcggaccgtg ctcaggcgat
     2701 gtgcaatgac gatgacggtg cgccccttgc agatctgcgc catgttctgt tgaatgatcg
     2761 cttcggattc atagtccaga gcgctagtgg cttcgtcaaa gatcagaatg cgaggattcg
     2821 caacgagagc acgagcaatg gcgatacgct gacgttgccc gccggagagg gcgcagccat
     2881 gctctcccac aatcgtgtca tatccgtccg gaagctccag gatgaactcg tgggcacccg
     2941 ccagtttggc cgcatgcatc acacggtcca ttgagagccc cgggtcggtt aatgcgatgt
     3001 tgtctcgaac ggagcgattg aaaagaaagt tctcctgcag taccacgccg acttgccgac
     3061 gcaaccaggc cggatcgacc tgcgcaagat ctaccccatc aaccagcaca cggccgcgtt
     3121 ccggcacata caaccgttgc atcagcttcg cgatggtgct tttgcccgac ccggatcgcc
     3181 ccaccatccc aataacctgg cccggctgta gggagaagga gacctgctgg agaaccgggc
     3241 ggccatctgg acgatagcga aaggtgacgt cgtcgaacgt aatctgcccg gcgatccgcg
     3301 gtaacgttgt gcgggccggg ctatatgcag gttcgggccg ggtgttcaac acatcgccca
     3361 agcgttgtat tgaaatgccc acctgttgaa actcctgcca gaggttcacc atacgcaaca
     3421 agggtccggt cacttggccg gcgagcatgt tgaaggcgat caactcgccg atactcagcg
     3481 ctccgtcgat gacctgatac gcaccgaccc acatgacggc aatggtcgta gttttttgga
     3541 taaaagtcgc cagctggccg gctatggtga tcagactggt cgcacggaaa ctggcctgca
     3601 catatccggc caattgttcg tcccagcggc gctgcaacgg tggctcgacg gccagagcct
     3661 tcacggtctg aataccgcct acggcctcga ctagaaatga ttggttctcc gctccgcggt
     3721 taaacttctc attgagccgt gtgcgaatcg tcggcgtgat ggcaatcgag agaatggcat
     3781 agagcggcag ggatgccatc accaccagcg tcaacatcga actgtagagc cacatcacag
     3841 cgataaacac ggcgatgaac acgacatcca gcacgactgt caccgaacta ctggtgagaa
     3901 attgacggat atgttcgagt tcgcgcacac gtgcgaccgt atcgccaacc cggcgtgctt
     3961 caaagtaggc gagagggagg gccaaaaggt gccggaacag ttgcgccccc aaactcacat
     4021 cgatccggtt cgtggtgtga gcaaacagat aggtacgcaa cccgccgagc agtccgtcga
     4081 ataatgccag cgccagcatg ccgatggcca taacatgcag cgtcgtgaag cccttgtgaa
     4141 ccaaaacttt atcgataacg acctgggtga acaggggagt gagcagtgca aagagctgta
     4201 gaaaaaagga cgccaacaac acttcgccca gcagccgtcg atacttgaat accgctggaa
     4261 tgaaccaagt gatgtcgaag gcaagatcct gcgcccggag acccgcgcgt ttcccacaga
     4321 gtagcaacct gccactccaa tttgtgacga acatctctct cgattgaacc agtgatcgcg
     4381 cctccagtgg atcatggatg agcaccttgt cgtcctgcac tttggccagg acgacgtatc
     4441 gcccgtcgat tcgttggact atggccggca atggcgttgt cgcaagctcc tcccatcgag
     4501 cagtcacaag tccgactcga aacccaatgt gtttggctgc gcgtagcaac tcctgtgtcg
     4561 ccagtacctt gcccgactgt ccgaactgat gctgaatctg tcccccatca gcaggcaggt
     4621 cgtggaacct ggcgaccatg agcaggcata acaagcccgt atctgttatg ggacttggca
     4681 cagagcaatc cgcgggattt gacgtcaacg tatccatggg gaagatgtcg gtccgacagg
     4741 ttaggattgt ttggtcttag caggttggag actgaccagc acccgctgaa gttgctggta
     4801 gagacgcacc acggaatcga gaggtaaggc aacacgtgcc gcgctgattc cctcgacctg
     4861 tttcggtgtg acttcgcctt gcttcgcacg cgtggcgacg gcgctcaaca aagcaggctc
     4921 taaaaaccca aaatcgacat atgccagtcc gtccgagaga ccaacatgag tgtagttcac
     4981 aagaatcggc tgatccgatg gggaaacggg tttaagacga acattcacgg ccaccgattt
     5041 atccccacgc tgcataggat gatgactttc tgccatgctg ctcctccttt atttcactaa
     5101 gcgtgatgca atcttcgcaa gtcgcccgga ctctcgcagg acagaggcga tgttatggcg
     5161 agactgcccg gcgcactatt ccccggaggt tgggcagagg caaaaacttt ggtaggccaa
     5221 tatatttccc taggtatatg gaggttaccc cacgcagtga aggttagaat gcagggctga
     5281 acaatccgaa acgtgtagga caaatgcgga ctcgccagga gtgacactgc gccaattctc
     5341 cgattcagaa cgtgtcacta tcattcgctg ggttggagaa gatgcagaca tgcatatgcc
     5401 accatgtcgc agattatggg gcttggcaac acatggatat cgccattctc ccctacaatt
     5461 aggatcagcg gcggaccggc tcttgtcaca gccagggcgt tccatctacc gctcgtagac
     5521 tcaccgggaa cgcacttacg tcctcagaca acgggcttac cctaccgccc cgttcgcgga
     5581 cttcctccag tgttttcatc agtcccctcc tcaaacacga ggggattttg cttcgttttg
     5641 aatcgacgga gctcctgcca ggccggcacg tgggaaccgt gggcctgaca aaagacgggt
     5701 gcgtgatggt ctggaagtcg acgcccgagc ttacccatgg acgaatcggg tttagcctga
     5761 cctacattca gaaactcgag catcaagacg gagaaaagtg ggttgaggtt ccgctcacac
     5821 cgatggtgca ccatgaaccg aacaagtgcc aagagcctac ggggtaagca cggcgtggtt
     5881 cttcactcgg cgcagtctcc tgcacccggc gttgcctccc ccccattcac tacgcaacgg
     5941 cattggtgct tgttatgata aaaccacggc tgtgatgacc cgtgctgacg atctgaagcc
     6001 cgatccttac aacacacccc aagtgcacac tatgtacaca ccgattaata aattgctgat
     6061 gcaaggcgtg gtagacagct ggatccgatt gagcgcgtcg ttggcggttt gggcattctg
     6121 ccttaccgtc acagcctgtg cgggcgtgga gcacaaggac acattgccga cagtggcttc
     6181 ggtagatctc gcccgctacg cgggaacgtg gtacgagatc gcccggctac cgatgtggtt
     6241 tcagcggcac tgcgttgact cgaaagccat ttataccagc cgtccggacg gcaggatcgg
     6301 agttcacaat gagtgtctca ccgacagtgg cggagttgat caggcggagg gtgtggcgac
     6361 ggtgatcgac cctaaaacca atgctcggct cacggtggtc ttcgataact tcttcgcgcg
     6421 gctcttcggt gcatcacggg atgggaacta ttggattctt gcgcttgatc cggaataccg
     6481 aaccgcgttg gtcggcacac ctgatcgtcg gtttctctgg attctctcgc gagcacctca
     6541 gctggaagag gttacctatc agcgcctcgt cgagcaggcc aagcgcctcg gctttccaac
     6601 cgcagatctc attcgcactc gccgaccggc atcatgatcg cctaagcttg ccggccgccc
     6661 tgaggaccac ggctgcaacg ggaaggctcg cttcgcactt gccacacaga gtcagctctg
     6721 cctgcgtccc tcgcgtagga gggtttccag ccgctgccgc aacgcgccga tctccttcga
     6781 cttgaacgag ctgcgcgtac gctccatgac ctcgacggcg tcctgaatgg ccgcctcata
     6841 ccgatccagc ctggggcact gcatcaatgc acagacgtcc gtgggagctt gaggcctgct
     6901 cccacgcgaa gcttccaacg tttgcgcgac gagtgccgac aactgggcga tgtgtcccat
     6961 ggcctgggcc atatactcat gggcgctcca ttccacctgt ggttctcccc gcccgaattc
     7021 tctcatcgac tggcccagtt gatcgaaaat tcccgccacc ttggccgttt cggtcatggc
     7081 atcctttacc gcacgcgcaa gggtccttcg ccgaagcccg cgctccaccg cccgcttcag
     7141 atcgtccacg ccgaccggtt tgatcaggta gtccacaatc gagaggcgaa acgactcgac
     7201 ggcagtcccg accgtcggat accccgtaat caccaccaca ggaagctcgg gatgtcgcct
     7261 ggaaacgttt tcaagaaact caaggtgagc attccccggc atccggacat cggccagcag
     7321 caagtcgtag ccgttttcca gcaggctttc ggcctcagcc gcatctcggg cagcactaca
     7381 cgcgtatcct gcctcagcga gaatttccgc cgtagattcc cgaaacgtct cttcgtcatc
     7441 agcaatgaga atgcgttgtg gcataatcgt catctttctg ctccttgtct gcattcactc
     7501 ccgccgctcg ctcccccgac atatccggcg gttccaaggg atacaccacc gtgaacgtcg
     7561 acccacgttg taattcagtc tggacttcga tccgcccccc catggcttgc accagcccac
     7621 gcgacacaga taacccgagt ccgagtccct gccaaccggc accaggcttc gtcgtaaaaa
     7681 atggctcgaa catacgggga agtacgtccg gagctatccc cgtcccgcaa tcagtcacgc
     7741 tccatcgcag cagcccctcg cgctccgaca cgttcagcac cacctcactg tcggggggag
     7801 aggcatcgat ggcgttctgc acgagattga gcaacacctg aaagagatcg gagcgcgaca
     7861 cgttaaccac ctgaaggggt gtatccacct ctgtgtgtaa ccggagtcgt ttcacggcca
     7921 tcttatgctc cagcaacgtg gccacgtcct gaagcaattc ggctatgtcg gtggcctgtc
     7981 gctcggtcgc cgggaggcct tgatacaggg tatacatctt tttcacgatc gcggtaaggc
     8041 gttgaatttc gcgatcgatg agatccacga atcgatagtg tcgatgctcg ctcccgattc
     8101 cctgcttcac caggtgaaac gcattggtaa tccctgccag gggattgttg acttcatggg
     8161 cgatatcagc cgcgaggtgg cccagcgccg cgagtttttc tgcctgagac cgctgggatt
     8221 caagttgctg aatcctcgcc gttcgctcac gcacgcgttg ctccagctga ctctgcacct
     8281 ccagcaaggc ctgctccgcc cgacggcgct cggtaatgtc atgaaaagag ccgacgacgg
     8341 cgtaaggtcg cgcgtcactg gctctcagga tcgcctgcgt attgattgag atccatcgct
     8401 gttccccggt cggtcgagag agacccataa tcacattcga gcacgggcga ccggtccgaa
     8461 gcgtgaccat cggcgggcgt tgatcaggag caaagtggct cccatcctca tgaatcgcct
     8521 gccatcccgg atcgaccggc gtccgcccca tcagctggtc ggccgtgagc cc
//
LOCUS       DAORRQ010000108         8442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_351_length_8442_cov_17.605342, whole genome shotgun sequence.
ACCESSION   DAORRQ010000108 DAORRQ010000000
VERSION     DAORRQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_351_length_8442_cov_17.605342"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK224_19495"
     CDS             <1..235
                     /locus_tag="PK224_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17990.1"
                     /translation="IGWACVWIVLAAQSKRDGSLCPKRTTFVVTLVAAGVLFVTRTID
                     PSVLVQHVMQILFILAIAIASYYAGCRNWLGKW"
     gene            complement(339..839)
                     /locus_tag="PK224_19500"
     CDS             complement(339..839)
                     /locus_tag="PK224_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17991.1"
                     /translation="MVQRTAEVTVQHDNSSGPADHRGLPAGAVPGPDLIASNGPTPLR
                     CPTHKCDGFLNADVSGYDSRTGLEVLVCTQCGHRGFRSREGVILLFRGGYEFKFSYGP
                     SLQTVTVVLSSASVNLWSTHGVNDEQLAKIAAEWSLLCGNTTKRVHLGIPAEEFADFY
                     LYFCQK"
     gene            1164..1709
                     /gene="lexA"
                     /locus_tag="PK224_19505"
     CDS             1164..1709
                     /gene="lexA"
                     /locus_tag="PK224_19505"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRB17992.1"
                     /translation="MTPDEFKRARLALGLTQQALADQLKRKRLAIARYEGGTRTIPGV
                     VEVALQSIGQSSRIPLVGIVAAGDPIEPIPQTERIEVPTSMLGRGETFALRVKGTSMR
                     DDGILPGDVVVVQKQDGARNGQTVIALLNGEATIKTYHRTAGVIELHPANEAMTPILV
                     HETDSFHIEGIVVGVIRHLKR"
     gene            1741..2925
                     /locus_tag="PK224_19510"
     CDS             1741..2925
                     /locus_tag="PK224_19510"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17993.1"
                     /translation="MDRQIVCFAIPSLEIALARLSQLSLRTRPLAVAAIDTPRALLRE
                     VSTEAEREGLYVGMTLDRARHLCPSLHVSPPNPRQVATANHSILSVIQRYAPTWEPFL
                     PGSLMMDLTGTTRLFGSACDVAAKIQADVLTQFHLEGVAGVGTNKLVAQTAATLVEPS
                     ELYDVRSGSERLFMSPLSIRTLPGVHRPCMRIVLKRLDDLNLRSLGDVADSPLDALEV
                     VLGNYAGQLSRWAQGIDPTPVLPPVAHPALNDTITLNPDEVDDARLQRRLADSLQQLC
                     RTLRSQRRICGGLSLTLRYSDQREVTTKARVIPETCWEIDLSGLLQGLFQRAFRRRIR
                     LRMMTVTLTDLTGFAEQGSLFEERPPEEQWARNRSQRLAVALDHLHQRFGEQAIRYGR
                     TQ"
     gene            2926..6027
                     /locus_tag="PK224_19515"
     CDS             2926..6027
                     /locus_tag="PK224_19515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRB17994.1"
                     /translation="MSSGFVHLHTHSSYSPMWGIPTLEALCQAARTQGQDRLALTDTN
                     GLYGAIRFLEVAKHEGLKPILGAELVHKAHRAVLLAKTPAGYANLCRLLSARHEESTF
                     DFIAAVARYRAGLIILSDDLSALRTWRKDSPKDVYVELTPGSDIQEAITFSRRNGLSP
                     VATTRAACLHPTDFEAHRLLRAIADNTTLSRLRPERCCAPSHWLMPPTVIERYLPHVS
                     EALTNSRRIADDCFTDWSFKETIFPLFRQLSAEAAFESLRTKTYEGAQRRYGTLSETV
                     RHRIEAELAVIREKRYADYFLVVDEIVREAPRTCGRGSAAASIVSYCLGITHVDPIRH
                     NLLFERFLNPGRHDPPDIDIDFPWDERPKILEWVFVRYGAKQAAMVANQNTLAPRAAM
                     REIAKVYGLPAAEIGKALDLLHRRADFVNVTEGSTLQTWASEVCRALQLRPPWPDILF
                     RAGQLQGHFRHLSLHPGGVVLVPDEIRRYVPVETSASGWPVIQWEKDQAEDAGLVKID
                     LLGNRSLAVIRDALAAVHHNTGRLIDYELWDPISDPVTQELIRRGDTMGCFYVESPAT
                     RLLLKKLWTTMPQARRAHADVFEYLVVVSSIIRPAANVYADDFIRRSHGHPYRSWHPL
                     LDEVLAETHGIMVYQEDVMKVAVALGGFSVHDGDQLRKILSKKHKERQLRDYQRQFYE
                     GAAARDVHRSTTDKIWAMIMSFSGYSFCKPHSASYAQVSFKSAYLRAHYPAEFLAAVI
                     SNQGGYYSAFAYLSEGRRMGLTIFPPDINASDWAYRGSDRAVRVGLMQLKALREEFAQ
                     RIVVERQDHGAYRSLQDFLDRLKPEIAQATLLIKAGCFDSIAGELTRPALIWRLFAAQ
                     SEKPAGYLPIPPEYSAQQKLSHELELFGFPLSGHPLELFADRVAGLSHLPARDLAQHV
                     GKHVTLLGWMVTEKIVSTKQGEPMEFVTFEDQTSLYDATFFPDIYRRCCHLLASDQAY
                     LVTGVVEEHFATVTVTVTRLRPLSAPGAEDTIVLEEDAGEPYTERHDTLSPDPLRHLH
                     LDL"
     gene            5978..6868
                     /locus_tag="PK224_19520"
     CDS             5978..6868
                     /locus_tag="PK224_19520"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRB17995.1"
                     /translation="MTLSLLIRFGTSTWTYEGWKGQIYTRTYTKTGFTRDCLEEFCQY
                     LYKGEPLFRTVGNDATFYRPPTVNQLIRYLSQIPEDFEMCAKVWEEITIPTYATHARY
                     GIKAGQRNPNFLNADAFIKLVLQPYRDARFGPHTGPFLFEFQRHGLSVQEFCSGLDGF
                     FARLPKDFRYAVEIRNAEMLGPQYQEVLRRHGVAHIYNHWSFMPSLAEQHQRMGIFTA
                     PFSVFRLQTPLKMTHEAAKTRAEPYNRIVGELPEMRQDTVRLVRQAAGENRTTYVLVN
                     NRAEGNAPLTIQALVESLRE"
     gene            7148..8425
                     /locus_tag="PK224_19525"
     CDS             7148..8425
                     /locus_tag="PK224_19525"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRB17996.1"
                     /translation="MSPVATPTTPPLQLITIDRRVEDHHSSCKIPAPGTQAPTKRHTY
                     RMMALAAVHAYIVFHLISWHVFGIELWGKTAMMGVPSLAKGTINAASIMVLLILGSIL
                     IWGRGFCGWACHMRGAIEFADWILRKLKVRRYLALREKNILVNTPHRWLLRIGALFVL
                     LLPVIILIHNAGFTPKVNVMTPPPIADLPGYEGKAFAKAAFFNFEIKPTWNDFLLAFG
                     LAVFIQFTMSVLLNLRYGQGAFCRILCPYATMMVPLMNISPVQKKITRVAQCTGCRDC
                     SNACPQGIDVSREIFHFNGKVVNTECIKCYACIDACDDNVLQDTAAPGVPQTDHLKPY
                     EKRPWQQELIRKDGLLTNARHMQVFEPLGPVADFSSMIVALICGGITSRFGGFWFYPG
                     AIISFIVFRECCLRVQRWRAVERIQTSIVSVKS"
BASE COUNT         1781 a         2620 c         2292 g         1749 t
ORIGIN      
        1 gatcggctgg gcctgcgtgt ggattgtgct ggcagctcag agcaagcgtg acggcagtct
       61 ctgcccgaag cgcaccacgt tcgtcgtgac gttagtggct gccggcgtgc tcttcgtgac
      121 tcgaacgatc gacccgagcg tgctcgtcca gcacgtgatg cagattctgt tcatcctggc
      181 catcgcgatt gcgtcgtatt atgcaggctg tcggaactgg ttggggaaat ggtgatcgcc
      241 atccggccaa ctctgcttcg gatgaccgaa aagtaaatcg ctgggggaaa ctgcggcgga
      301 gacgggagga agacctgatc gtatcgactg cgaccacgtc acttttggca gaagtagaga
      361 tagaagtcgg caaattcctc agcgggaatg cctaaatgca cccgcttcgt cgtattgcca
      421 cacagcaggc tccattccgc cgcaatcttt gccagttgct catcgttcac accgtgcgtg
      481 ctccacagat tgaccgacgc agagctgagc acaacggtga ccgtctgcag tgacggcccg
      541 tagctgaact tgaactcata cccgcctcga aacaagagga tcaccccttc acgggatcga
      601 aaacctcgat gcccgcactg cgtgcacacc aacacttcca accctgtacg agaatcgtat
      661 ccggagacat cagcattcaa gaacccgtca catttgtggg tcggacagcg aagaggggtc
      721 ggaccgttcg atgcgatcag gtcaggacca ggaactgctc ctgcgggtaa tccccggtga
      781 tctgccggcc ctgaagaatt atcgtgctgc acagtgacct cggcggtgcg ttgtaccatc
      841 cctggatctc ccgcaagagc gcgtcaaaag tacgatgcca cggtcaacta tggcaagtat
      901 atcagacatc gtcataaggc caggacgcgg aataaaccag caaatgccct tcggaaaaag
      961 gtccgaaacc tagaagatcg atgagtgtgc cgccccactc tagcacgttg agtttcttca
     1021 taaagccttt tgacagtggt caatgtgtgc agagaatggt tcgcacatcg caccggctca
     1081 aattgcacgc tggcaggtcc tgcgaccgtc gccctcacaa tcaccattga cagaaacagc
     1141 ccggccatgt atccattgga tacatgacgc ccgatgaatt taaacgcgcc aggttggctc
     1201 tcggcctcac ccaacaagcc cttgcggatc agctcaaacg caagcggctt gccattgcca
     1261 gatatgaagg cgggacacgc acaatccccg gcgtcgtcga ggtggcgctg cagtccatcg
     1321 gacaatcatc ccggattcca ctcgtcggga ttgtggccgc aggcgatccc atcgaaccca
     1381 tcccccaaac cgaacgtatt gaggttccca cgagcatgct cgggcgagga gaaacttttg
     1441 ccttacgggt caaggggacc tccatgcgcg acgatggcat tctccccggc gatgtggtcg
     1501 tcgtccagaa acaggatggg gctcgaaacg gccaaaccgt gattgccctt ttgaacggcg
     1561 aggccacgat caagacatat caccgcaccg caggcgtgat cgagttgcac ccggccaacg
     1621 aagccatgac acccatcctg gtgcacgaaa ccgacagctt ccacattgag ggcattgtcg
     1681 tcggcgtgat ccggcatctg aaacgatagg tgcgctcatc agaccgcgtg agcgaagacc
     1741 atggatcgtc agattgtctg ctttgccatt ccctctctgg aaatcgcgct agcacggttg
     1801 agtcagctat cactccgaac ccgtccgctc gcggtcgccg cgatcgacac tcctcgtgcg
     1861 cttctccgtg aggtctccac cgaggcggaa cgagaaggcc tctacgtcgg catgacactc
     1921 gaccgggcgc gccacctctg cccatccctg cacgtcagtc ccccgaatcc tcgtcaggtc
     1981 gccaccgcca accactcaat ccttagtgtt atccagcgtt atgccccgac ctgggaaccc
     2041 tttctccccg gttctctcat gatggacctc accggtacca cacgcctctt cgggtcagcc
     2101 tgtgatgtgg cggccaagat tcaagcggat gtactcacgc agtttcacct cgaaggcgtg
     2161 gccggtgtgg gtacaaataa actcgtcgca cagacagccg caaccctggt ggaaccctct
     2221 gaactctatg atgtccgctc gggatcggag cgcctcttta tgtctcctct ctcgattcga
     2281 accttgcccg gcgtacatcg cccctgcatg cgcatagtgc tgaagcgatt ggacgatctc
     2341 aacctccgct cgttgggaga cgtggctgac agccccctcg acgccctcga agtggtgctg
     2401 ggcaactatg ccggacaact atcccgatgg gcgcagggca tcgacccaac gccggtcctt
     2461 ccgccggtcg cccatccagc cctgaacgac accatcaccc tcaaccccga tgaggtggac
     2521 gatgcacggc tccagagacg actggcagat agcctgcaac aactttgtcg caccctccgc
     2581 agccagcgcc ggatatgcgg cgggctctcc ttgacccttc gctacagcga tcaacgcgag
     2641 gtgaccacga aggcgcgggt gattccggag acctgttggg agatcgatct ctccggactg
     2701 cttcaggggc tttttcaacg agccttccgg cggcgtatcc gtcttcgcat gatgacggtc
     2761 acgttgaccg acctcaccgg ctttgctgag cagggatcgc tgttcgaaga acgaccaccg
     2821 gaggagcaat gggctcgcaa ccggagccag cgactcgccg tcgccctcga ccacctgcat
     2881 caacggttcg gtgaacaggc gatccgttat gggagaacgc agtaaatgtc gtccggcttc
     2941 gtgcatctcc acacccattc atcctattcg cccatgtggg gcatcccgac gcttgaagcc
     3001 ctctgtcagg ccgctcgcac acaagggcag gacaggcttg ccctcaccga taccaacggc
     3061 ctgtatggag ccatccggtt tttagaggtc gcgaagcacg aagggttgaa accgatcctc
     3121 ggggctgagc tcgttcataa ggcacaccgt gcagtgctgt tggccaaaac gccagctggt
     3181 tatgccaatc tctgccgtct tctctccgct cgtcacgagg agagcacctt cgatttcatc
     3241 gcggctgtgg cacgctatcg agccggattg atcattctgt ccgacgactt gtcagcacta
     3301 agaacctggc gcaaggactc accgaaggat gtgtatgtcg aactcacgcc cggatcggat
     3361 atccaggagg ccatcacttt cagccggcgc aacgggctgt caccggtcgc taccacccgc
     3421 gccgcctgcc ttcatcccac agacttcgag gcgcatcgcc tgctgcgcgc catcgccgac
     3481 aacacgacat tgtcccggct ccggccggag cgctgttgtg cgccatccca ttggctcatg
     3541 ccgccgacag tcattgagcg gtatctgccg catgtgtccg aagcgcttac caacagccgg
     3601 cgtattgcag acgactgttt caccgactgg agcttcaaag aaacgatctt ccccttattc
     3661 cgtcaacttt cggcagaagc cgcatttgag tcgctgcgca cgaagaccta tgagggcgca
     3721 cagcggcgct atggcactct gtccgagacg gtgcggcacc ggatcgaagc agagttagcg
     3781 gtgattcgag aaaaacgcta tgccgactac tttctggtgg tcgatgagat tgtccgggaa
     3841 gcgcctcgta cctgtggacg agggtccgcg gccgcctcga tcgtctctta ttgtctgggc
     3901 atcacccatg tcgatccgat tcggcacaat ctgctgttcg agcgcttcct gaacccaggc
     3961 cggcatgacc caccggatat cgacatcgac tttccatggg acgaacgtcc caaaattctc
     4021 gaatgggtct ttgtacgata cggagcgaaa caagctgcga tggtcgccaa tcaaaatacc
     4081 cttgccccgc gtgcagccat gcgtgagatc gccaaggtct acggcctgcc ggccgctgag
     4141 atcggcaagg cgctggatct gcttcatcga cgcgccgatt tcgtgaacgt gaccgagggt
     4201 tcgaccttac aaacctgggc aagcgaagtc tgtcgcgccc tgcaattgcg cccaccctgg
     4261 ccggacattc tcttccgggc cggacaactg cagggacact tccgccatct cagcctccac
     4321 cccggcggag tggtcttggt tcccgatgag atccgccggt atgttccggt ggaaacctct
     4381 gcttccggtt ggcccgtcat tcaatgggaa aaagaccagg ctgaggatgc ggggctggtc
     4441 aaaatcgacc tactcggcaa tcgttccctc gccgtcattc gtgatgccct ggctgctgtc
     4501 caccacaaca ccggccgcct gatcgactac gaactctggg atccgatcag cgatccggtc
     4561 acgcaggaat tgatccgccg cggggataca atgggctgct tctatgtcga gtctccggca
     4621 accagactcc tgctcaaaaa actgtggacg accatgccgc aggcgcgccg ggcccatgcg
     4681 gatgtctttg aatatctggt ggttgtgtct tccatcatcc gtcccgccgc caatgtctat
     4741 gccgatgact ttatccgccg atcccacggc catccctacc gctcgtggca tcccttgctg
     4801 gatgaggtct tggctgagac gcacgggatc atggtctacc aggaagatgt gatgaaggtg
     4861 gcggtggcct tgggcggatt ttccgtacac gacggtgatc agctgcgcaa gatcctcagc
     4921 aagaagcaca aggaacggca gctgcgagac tatcagcgac agttctacga aggggctgca
     4981 gcacgagacg ttcaccgatc gacgaccgac aagatctggg ccatgatcat gagcttttcg
     5041 ggctatagct tttgtaagcc gcacagtgcg agctacgccc aggtgtcctt caaatcggcc
     5101 tacttgcggg cacattatcc ggctgagttc ctggccgccg tcatcagtaa ccaaggcggg
     5161 tattattccg ccttcgccta tctgtcagag gggcggcgca tggggctcac gatctttcca
     5221 ccggacatca acgccagcga ctgggcctat cgtggatcgg accgggccgt ccgggttggc
     5281 ctgatgcagc tcaaagccct acgcgaagaa ttcgcccaga ggattgttgt ggagcggcag
     5341 gatcacggcg cctaccgctc gctacaggat tttctcgacc gactgaaacc cgagatcgcc
     5401 caggccacac tgctcatcaa agcaggctgt ttcgattcca tcgccggaga actgacccga
     5461 ccggcgctga tctggaggct cttcgccgcc cagtcagaga aacctgccgg ttatctgccg
     5521 attccaccgg aatactcggc tcagcagaag ctcagccatg agctggagct gttcggcttt
     5581 cccctgagcg gacatccact ggagctcttc gcagacaggg tcgcgggcct gtcgcatctc
     5641 ccggcacggg acctggcaca acatgtcggg aagcacgtga ccctcctcgg ttggatggta
     5701 acggagaaaa tcgtgtccac gaagcaaggc gagccgatgg agtttgtcac ctttgaggat
     5761 cagaccagcc tgtatgatgc cacctttttt cccgacatct accggcgatg ctgccacctc
     5821 ctcgcctcgg atcaggccta cctcgtcacc ggtgtggtgg aagaacattt tgcgaccgtg
     5881 acggtcacag tcacacgact tcgcccctta tccgctcccg gcgcggagga taccatcgta
     5941 cttgaggagg acgcgggaga accgtacact gagcggcatg acactctctc tcctgatccg
     6001 cttcggcacc tccacctgga cctatgaagg ctggaagggg cagatctata ctcgcaccta
     6061 tacgaaaacc ggcttcacac gggactgtct cgaggaattc tgccagtatc tctacaaagg
     6121 cgagccactc tttcgcaccg tggggaatga tgcaacgttt taccggcctc ccacggtcaa
     6181 ccagctcatc cgttatctaa gtcagattcc ggaagacttc gagatgtgcg ccaaggtctg
     6241 ggaagaaatc accatcccca cctatgccac acatgccagg tacgggatca aagccggaca
     6301 gcggaatccg aactttctca acgcggacgc cttcatcaag ctggttttac agccctatcg
     6361 ggacgcacga ttcggcccgc acacagggcc gttcctattc gaatttcaac gccacggact
     6421 gtccgtccag gaattctgca gcggattaga cgggttcttc gcgcgcctgc cgaaagactt
     6481 ccggtatgcc gttgaaatcc ggaatgcgga gatgctgggc cctcaatacc aggaggtgct
     6541 caggcgacat ggcgtggcgc atatctacaa tcactggtcc ttcatgccgt ccctcgccga
     6601 acaacatcag cgaatgggga tctttaccgc accgttcagt gtgtttcgtc tccagacccc
     6661 gctcaagatg actcacgagg cggcaaagac acgtgccgaa ccgtacaaca ggattgttgg
     6721 tgagttgccg gagatgcgac aggacacggt acggctcgtt cgacaagcag ccggcgaaaa
     6781 tcgaaccacc tatgtactgg tcaacaaccg ggcggaaggc aatgcgcccc tgacgattca
     6841 ggccctcgta gaatcactgc gcgagtaagg gcaccttctg acagtgtgat gttggcccat
     6901 cacctgcttc ccacgcaggc gccctccaat tcgacccggc tatccccgct ctaagctttc
     6961 ccatcaccac acgcaaccgg accatccggt ccaggggttc attcgacgag cccatacctt
     7021 tatcgcgccc gcctccgtct ttcgatgcat agcgcagact gctgatcgcg actattcttc
     7081 atcaagagcg tttcacaacg gataccaagc cgaggtggga atctcctacc accttcaggg
     7141 ggtatatatg agtcctgttg ccactccaac cacaccgccg ctccaactca tcaccatcga
     7201 tcgccgcgta gaagaccatc acagctcctg caaaattcct gctccaggca cacaggcgcc
     7261 gacaaaacgc catacctacc gtatgatggc gttggccgcg gtgcatgcct atatcgtgtt
     7321 tcaccttatt tcatggcatg tcttcgggat cgaactctgg ggaaagacgg ccatgatggg
     7381 tgtaccgtcc ttggccaaag gaaccatcaa cgcggcctcg atcatggtgc tgctgatctt
     7441 gggctccatc ctgatctggg ggcgtggatt ctgtggctgg gcctgccaca tgcgcggagc
     7501 gatcgaattt gccgattgga ttcttcgtaa gttgaaggtc cgccggtatc tcgccctccg
     7561 agagaagaat attctggtca acacccctca ccgctggctc ctgagaatcg gggccctgtt
     7621 cgttctcctc ctgccggtca tcattctgat tcacaacgcc ggattcacgc ccaaagtcaa
     7681 tgtgatgact ccaccgccta ttgcagactt gccagggtat gaaggtaagg catttgccaa
     7741 agccgccttc ttcaatttcg agattaagcc gacgtggaat gacttcctgc tggccttcgg
     7801 gttggcggtc ttcattcagt tcacaatgag cgtcctgtta aacctgcgct acggccaagg
     7861 cgcattctgc cgaatcctct gcccctatgc cacgatgatg gttcccctca tgaacatctc
     7921 cccggttcag aaaaagatca cgcgcgtggc tcagtgcacc ggctgccggg attgcagcaa
     7981 tgcctgcccc caaggtatcg atgtcagccg tgaaattttt cacttcaacg gcaaggtggt
     8041 caacacggaa tgcatcaagt gctacgcctg catcgatgcc tgcgatgata acgttctgca
     8101 ggatacggcg gcgccgggcg tgccgcaaac cgaccacttg aagccgtacg aaaaacgccc
     8161 ctggcagcag gaactgatcc ggaaagacgg gctgctcacc aacgccagac acatgcaggt
     8221 gttcgaaccc ctgggacccg tggctgattt ctcatcgatg atcgtggcgc tgatctgcgg
     8281 aggaatcacg tcccgcttcg gaggcttctg gttttacccc ggagccatca tttcgttcat
     8341 tgtgttcagg gaatgttgtt tgcgcgtgca gcgatggcgg gctgtggaga gaatccagac
     8401 atctatcgta tccgtcaaat cgtgatgtgt cgaccggcag tc
//
LOCUS       DAORRQ010000109         8409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_355_length_8409_cov_99.166986, whole genome shotgun sequence.
ACCESSION   DAORRQ010000109 DAORRQ010000000
VERSION     DAORRQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_355_length_8409_cov_99.166986"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PK224_19530"
     CDS             <1..360
                     /locus_tag="PK224_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17997.1"
                     /translation="DQCNAGGFQKPPFVNSLLVEKSYWHCVKPKANHLVSVRVIPEGG
                     GQGKDVLRIVAMVGVIALAMAAPWAAGLAGSTLLSTGTLGGALLTASVGIVGSLAVTG
                     PIPTPLPCRPFPQLIED"
     gene            372..1001
                     /locus_tag="PK224_19535"
     CDS             372..1001
                     /locus_tag="PK224_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB17998.1"
                     /translation="MISRLQQFQHNEDGTISIKGQPGIVVEAIAEQDLALATAHALQL
                     EKVLGLPHYREDWERFAAYTGMSSRMVKFVLVDESISRLVGSPRYQPRNITWPASCAL
                     EFGHRGFLVGMLTSITQCFDLPAEALKTLGIDVPIEGACLIGEAQLFNSRLADKAWEA
                     IQRGIFSHVCAVLFRQPNESPGTGSVVEVSLVTGDFPGCRNARILKTWE"
     gene            complement(1345..2514)
                     /locus_tag="PK224_19540"
     CDS             complement(1345..2514)
                     /locus_tag="PK224_19540"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB17999.1"
                     /translation="MIYTSAIDQITWDAIVEFCQQGRTEDAYLDYKRDFPSDLANTIS
                     AMANTMGGIIIIGVQEDTESKPALPVPGIDFTRGLEERVTNIILGNIVPPVFPEVRVC
                     RNSENRALVLIRIQQSENAPHAVSRNTKIYLRTGNVNKPEDLAQIDQIEWLKNRRQKS
                     ENLRDSLFSRARQRASNQGNTLHLPLLTLAISPVYPNIPLTNPSELSAIRWDIEHKHG
                     IHSMPDPDNKCATFAHESIVIEDRDAHGSSYTELSIFGMYFSQACLLDITDSKKVMYL
                     DHIFIKLFLLLTSGEKLYSHLGYRGPVWFCCHLTSLQNRAMRGSYTLKMHSEKMCRDS
                     EVRLIDSLPMLPKDEDKIGFLSRVGRTIAWAFGWDLSEEDLTKHFNIMEKAVHVT"
     gene            complement(2988..3920)
                     /locus_tag="PK224_19545"
     CDS             complement(2988..3920)
                     /locus_tag="PK224_19545"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRB18000.1"
                     /translation="MVGVFFSYSHADEQYRDRLEKHLSPLKRDGLISQWHDRRIHAGG
                     NLHDEISKHVEEDQVILLLVSADFLASDYCQDIEMARAIERHKRKEAIVIPIIVHPCD
                     WKNTVLGELRATPQDGKPISKHPNHEDAYLDIVTDIRKAISALDNQPRQNKPKPTQTM
                     PTVPVQPSTRSSNLRIRKEFTDHDKDSFLDQTFDFISKYFEASLNELKTRNPDIETRF
                     KPQGQTFKTGIYRNGRKECWCRVSISDERHFGGGIAYSHTDSDSSFNEMLAIENDGYS
                     LILKPFMRSGGSYLTQEGAAEHLWSMFIEPLQQG"
     gene            complement(4470..4676)
                     /locus_tag="PK224_19550"
     CDS             complement(4470..4676)
                     /locus_tag="PK224_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18001.1"
                     /translation="MPAKPSKSETRDILLRDLPVSLADKIKVASTLHRSSMKDYIQEI
                     LLKHIEDLERKGVVLTLPGGKTKS"
     gene            4988..5131
                     /locus_tag="PK224_19555"
     CDS             4988..5131
                     /locus_tag="PK224_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18002.1"
                     /translation="MIKSRQEMHLHNLGDYQLVDRNGVVMGSNFDASLDQIETYSRYE
                     TKS"
     gene            5172..5468
                     /locus_tag="PK224_19560"
     CDS             5172..5468
                     /locus_tag="PK224_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18003.1"
                     /translation="MIKMANAEFASIENDALVRIERNADDLAGLLKGLESTAHILHIL
                     GMADNPLSIETPDLALMMRSFEEQLATIRDGVEGLSDMASEARTSKLSKVKAVR"
     gene            5494..5694
                     /locus_tag="PK224_19565"
     CDS             5494..5694
                     /locus_tag="PK224_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18004.1"
                     /translation="MAKPLVMYMAAHDPKDDVPMFPLGVMTTSKRTALKELREGRKHY
                     PGAYLVRVTHERCSEDTLKKGR"
     gene            5699..6331
                     /locus_tag="PK224_19570"
     CDS             5699..6331
                     /locus_tag="PK224_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18005.1"
                     /translation="MQAVHEGGKIQNPTRKKKSCREQIPRYDGWWKNPLPPMMEYVGP
                     IRCDYMTEFGISPSDIVIEEHRPTSLYDIRARSFVAINHSSFNVMIFAYVCDNSYREM
                     VIFADGNRQARLMETELRCCTFWNIPGVVKLPSQECYTYLRRRIYPGSMSVDRWRSEV
                     DEFFDDEKRRAKLNPVVTQTPKAKPPMQMPEIRSRGRLVQWPSVRNLVGK"
     gene            complement(7112..7411)
                     /locus_tag="PK224_19575"
     CDS             complement(7112..7411)
                     /locus_tag="PK224_19575"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRB18006.1"
                     /translation="MKTHRQYIKERIEKKPAFAQDLAEAEREVTIAVELAKLREQRGL
                     SQSQLARMTGMKQPQIARLESGAYMPAFGTLIRILEALKGKLELSPTECRVTCCR"
     gene            complement(8205..8280)
                     /locus_tag="PK224_19580"
     tRNA            complement(8205..8280)
                     /locus_tag="PK224_19580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8245..8247),aa:Arg,seq:acg)
BASE COUNT         2048 a         1971 c         2218 g         2172 t
ORIGIN      
        1 gatcagtgca acgccggtgg gtttcaaaaa ccaccgtttg tcaacagcct gctagttgag
       61 aaatcctatt ggcactgcgt caaaccgaag gccaaccatc tggtctcagt gcgcgtgatt
      121 cctgaagggg gcgggcaagg caaagatgtg ctccgcatcg tggcgatggt cggcgtcatt
      181 gcgctggcga tggcggcgcc atgggctgcg ggtcttgcgg gatcaacatt attgagtacc
      241 ggcacactcg gaggggcgct tctaacggcg agcgttggta tcgttggatc gctggccgtg
      301 actggcccta ttcccactcc cttaccttgc cgacctttcc cgcaactcat cgaagattaa
      361 gaggtagcgt catgatttct aggcttcaac aattccagca taacgaagac ggcaccattt
      421 caatcaaggg tcaaccaggg atcgttgtgg aggcgattgc agaacaagat ctcgccctag
      481 ccacagcaca tgcgctgcag cttgagaagg tcttaggact tcctcactac cgcgaagatt
      541 gggagcgatt cgccgcatat acaggcatga gttcacgcat ggtgaaattt gtccttgtgg
      601 atgagtcaat aagccgactg gtgggcagcc cgcgctatca gccgaggaat atcacctggc
      661 ctgcctcgtg tgcgctggag ttcggccatc gggggtttct ggtgggcatg ctgacgagta
      721 tcactcagtg tttcgatctg ccggcagaag cccttaaaac gcttggcata gatgtcccga
      781 tagaaggggc gtgtctcatc ggagaggccc aactattcaa ttcacgactc gccgacaagg
      841 cgtgggaggc cattcagcga ggcatctttt cgcatgtctg cgcggtgctg tttcgtcaac
      901 ctaacgaatc accaggcacc ggttctgtag tggaagtctc actggtcacc ggcgactttc
      961 cggggtgtcg gaatgcgcgg attttgaaaa cgtgggagta ggcgtggcgc ctaacttttt
     1021 ttggatcgca tacccagcca agtccgtccg accatgacga ggccgcacaa tacaataaag
     1081 ctggggatga acagccagta cagcagctga ggagtactca ttgacgagat catggctcag
     1141 tgtagcggac cggcatcaga ggtcaattat ttacccagta cttgttcatt gcgaccgatg
     1201 gtaggccgag atgtcggaat gcgaggattc tgaaggcggg catggctaac ggtgataaag
     1261 cttcgaggga tataccgcga taagatgatc tctggaaggt tgaagcagac agccgagatt
     1321 taggagatac aaagggattt ttcttcaagt cacatgaaca gctttctcca taatgttaaa
     1381 atgttttgtc aagtcttctt cggatagatc ccaaccaaaa gcccatgcaa tcgttctccc
     1441 aacccgactt aagaaaccaa tcttgtcttc atccttagga agcatgggga gggagtcaat
     1501 aagtcgtacc tcggagtcgc gacacatttt ctcactgtgc atcttaaggg tgtagctacc
     1561 tctcatggcc ctattttgca gcgatgttag atggcagcag aaccacactg gtcccctata
     1621 gccaagatgt gaatagagtt tttcccccga cgtcaggagt aggaatagct tgataaagat
     1681 atgatccaaa tacatcacct tcttcgaatc tgtaatatcg agaaggcatg cctgactgaa
     1741 gtacatgcca aagatgctta gttctgtgta tgaagaaccg tgagcgtcgc ggtcctcaat
     1801 cactatactt tcatgggcaa aagttgcgca tttattatca ggatctggca ttgaatggat
     1861 accatgtttg tgttcgatat cccatcggat cgcactaagc tcagatggat ttgtaagagg
     1921 tatattggga taaacaggcg aaattgcgag tgtgaggagt ggtaggtgca atgtgtttcc
     1981 ttggttactc gcacgctgcc tagcacgtga gaaaagtgaa tctcttaaat tttctgattt
     2041 ttgcctgcga tttttcagcc actcaatttg gtcaatctga gcgagatctt cgggcttatt
     2101 aacattccct gttctcagat atatcttcgt gtttctggaa actgcatgag gagcattttc
     2161 gctctgctgt atacgaatta aaacaagtgc tcgattttct gagttccggc aaactctgac
     2221 ttctgggaac accggaggaa caatattacc gaggataata ttagtaactc gctcctctaa
     2281 tccccgtgtg aaatcaatgc caggaactgg tagtgcaggc ttgctctcgg tatcctcctg
     2341 gacaccgatt atgatgattc ctcccattgt attggccatc gcggatatcg tgtttgccaa
     2401 atcactgggg aaatctcgtt tgtagtctaa gtaagcatcc tctgttcgtc cttgttggca
     2461 gaattctaca atggcatccc aagtgatttg gtcgatagct gacgtgtaga tcattgagat
     2521 aaatcaatat tggcaagtgt tttcactagt atgcagaccc gcccatcggc cagatcttat
     2581 tttttcttgc gattgtatgg cgactgattt gttggtttaa gcgatatttc ctcttccgag
     2641 gctttcgcac gaatggagtc ctcagtgcgt tggagtttta agccgattac tctcgtcgga
     2701 gtgttctcct tggctaactt cttaagggca gaaatttcag atgacttcca agactttcca
     2761 tgattcttcg gatttcccat agccccctcc attcattggt tataggttgg tcatatcgtg
     2821 ctttctcagc aaagaccgtt acttctcctg gattactgaa ccagaacatt tttcgcatcg
     2881 caccggaaaa gagggtttac ccttatcctc tgcctctcct ggttcgaaga cctctatggg
     2941 aaatatatga ccacaggatt tgcagacgaa acgacgcttg accatgatta tccttgttga
     3001 agcggctcaa taaacattga ccatagatgt tcggcggctc cttcttgagt gagatatgag
     3061 ccaccgcttc gcatgaatgg cttgagtatc aacgagtaac cgtcattctc tattgccaac
     3121 atttcattaa atgatgaatc cgaatcagtg tgagaatagg caatgccacc tccaaaatgg
     3181 cgttcgtccg aaatcgaaac tcgacaccaa cattcttttc tgccgttccg ataaatgccg
     3241 gttttaaagg tttgaccttg cggtttgaac cgagtctcaa tgtcgggatt cctagttttt
     3301 aactcattaa gtgaagcttc gaaatatttt gagatgaaat caaacgtctg atcaaggaat
     3361 gaatccttgt cgtggtccgt aaattctttt cttattctta agttgctact ccgagttgaa
     3421 ggctgaactg gcactgtcgg catagtttga gtaggtttgg gtttgttttg tcgcggctgg
     3481 ttatctagcg cggagatagc ctttcgaatg tcggtgacaa tatcgagata ggcatcttcg
     3541 tgattgggat gtttggatat tggtttccca tcctgagggg ttgctctcag ctcacctaat
     3601 acagtgttct tccaatcaca tgggtgcaca atgatcggga taactattgc ctcctttcgt
     3661 ttgtgtcgtt ctatagcccg cgccatttca atgtcctggc agtagtcaga ggccaggaaa
     3721 tcagcactca ccaacagaag gataacttgg tcttcctcga cgtgtttgga gatttcatcg
     3781 tggaggttgc cgccagcgtg gattcttcgg tcatgccatt gagatatgag gccatcgcgt
     3841 tttagtgggg atagatgttt ttctaatcga tctctgtatt gctcatcggc atgggaatag
     3901 gaaaaaaata caccaaccat ggatcaattc cctaatgtat ttctagcgtt cccgtcggta
     3961 acagaccctt ctttagtcca gtctcacgga attgcaccga agggcttcct tgtcttccat
     4021 gttgcacgta gcacgatact tcacttggat tcggagtgtt caccagtcgt ctgccagcga
     4081 gacaggagag agatggcctg atccttgaaa aagaagagcg tcagagcgaa aagcaatagt
     4141 cctacgatta cgggcactag tatggattcc caatgtgcat tcttggatgt ctgtatgggt
     4201 tgaatgaaga gtggtgaagg tgcttttctt tccggcaatc tatctaaaat gtcgtggcgc
     4261 atcttagtca aaacatctcg cagagatccg ctacagatga gaaccgtagc gattaataag
     4321 actatgaagg cttctatttc atgaatagca ctcctggcac ctacaaaaag gctgataccg
     4381 aacacgagga agagaatgcc cagggcttgc attttccctc aacgtaatgt ccgtcccata
     4441 ttgttagtca cggttaacac ggcacacaac tacgacttcg ttttcccacc tggcagcgtc
     4501 aacacaaccc cttttcgttc caagtcttcg atgtgcttta gcagaatctc ctgaatgtag
     4561 tccttcatcg aggatcggtg cagtgtcgaa gcaaccttga tcttatcggc caagctgacc
     4621 gggagatcac ggagaagaat atcgcgcgtt tctgatttgg atggcttagc tggcatgggg
     4681 cgggagggta ccacggaaaa cgcttgacat caaatgatat gcggtatagt ttgatatcat
     4741 tgattatctc atggggggat tctatggaga tgacacggat tattgtatcg attccgcgag
     4801 cattgaaagc caagctagac aggctcagag cgcaaggcac cacggcggcc ggcctgattc
     4861 ggcatctgct gaatcagcat ttcaatgaac gggtaaccac actcaaaagg aggggctaat
     4921 cgtgaaaaca atcgacgccg cacaagagaa cagggtcaga cagctcgccg ctcgcctgtg
     4981 atatcgggtg ataaagtcgc ggcaggaaat gcacctgcac aaccttggcg actatcagtt
     5041 ggtggatcga aacggagtgg tgatgggctc gaatttcgac gcctcactgg atcagatcga
     5101 aacctattca aggtatgaga cgaagagcta gccgaacatt tcaacgtgcc cacgaaaggg
     5161 cagaaaggac ggtgatcaag atggcgaatg cggaatttgc gtctatcgaa aatgatgcgc
     5221 tggtgagaat cgaacgaaat gcggatgatc tggcgggact attgaagggc ttagagtcta
     5281 cggcgcatat ccttcatatc ctcggtatgg ccgataatcc tctctcgatt gagacgcctg
     5341 atcttgctct catgatgcgt tcttttgagg agcaacttgc caccatccgc gatggtgttg
     5401 aaggtctatc agacatggcg tctgaagcga gaacctccaa gctcagcaag gtaaaagcag
     5461 tgagataggt tatttgcgaa tggagggtga ttcatggcga aaccactcgt gatgtatatg
     5521 gccgcgcacg atcctaaaga tgatgtcccg atgtttcccc tcggtgtcat gacgacaagt
     5581 aagagaaccg ccctaaaaga gctgagggag ggcagaaaac attatcccgg cgcctacctg
     5641 gtgcgggtga ctcacgaacg ctgcagcgaa gacacattga agaaggggcg gtgagaacat
     5701 gcaagcagtg cacgaaggag gaaaaataca gaatcccact cgtaaaaaaa agagttgtcg
     5761 agaacagatt cctcggtacg acggatggtg gaaaaatcct cttccgccca tgatggaata
     5821 tgtcggcccc attcgatgtg actatatgac cgagttcggt atttctccca gtgacatagt
     5881 cattgaagaa caccgtccca cgtcgcttta tgacattcga gcaagaagct tcgtcgcgat
     5941 aaaccacagc tcgtttaatg tgatgatctt cgcctatgtg tgtgacaatt cgtataggga
     6001 aatggtgatt ttcgccgatg gcaacaggca ggcccgattg atggaaaccg aattgcgatg
     6061 ctgcacattc tggaacattc ctggagtggt gaagctccct tctcaagaat gttacacgta
     6121 cttaaggcgc cgaatatatc cgggcagcat gtcggtcgat cgatggcgat ctgaggtgga
     6181 cgagtttttt gatgatgaaa agagacgggc gaaattgaat cctgttgtta cccagacccc
     6241 gaaagccaaa ccgcctatgc agatgccgga gattcgatca aggggccggt tggtgcagtg
     6301 gccatcagtt aggaacttag ttggtaaata gtccaggtct ccggctcgtg aaataaccca
     6361 aagagaaagg aatcttcgtg agctttcaag acgaaatggc gcccgatggt cagcatgtct
     6421 cgccggcctt aatcacggat atacagcggg tctgtaggga catcagggcg acagtcgggc
     6481 agattgcgaa cgcctgagcg cgcctagagc tcctagaccc tcagaaggtg acggtggcag
     6541 agtttaccgc ggggtgcctg gcatttcgac agcgtgctta ctgacagggc cttgtatgca
     6601 gaaaatgcag gatacgatgg aacggtatgg gggttgaagt gagggatgtg gtcccagttg
     6661 cgaccgtgga tcgctcagac gggcgggcgc gtttcttgcc cgtttgtgaa ccaaaaaagg
     6721 actctgacag gctgacagag atgctatgga ggcgaggctg aggcgcgcct cttttcatcc
     6781 ttattgtgga aattcccaaa aagcccgacg aggatcatca gattcgcgcg ccggcctacg
     6841 aatcgaagct ctcaaatcgt cccgctgtgc ttcagtgtac ccaggagaca cccattgagg
     6901 gcttccccaa tcaggggttc gcctgccacc ataagtgtca tgcgttctta tggtctcgat
     6961 ccatgtctcg acgcgatcag acggcgcatt cagtgacaca ttaactgggg cgtttattgt
     7021 agagtctccg cttatccacc cgcgcggggt gagatgccac actgtgcgct cgacttcggt
     7081 tcccattctt tactttgcta ccttaaatta ttcacctgca gcaggttacc cggcattcag
     7141 ttggacttaa ctccaatttt cccttgagag cttcaaggat acggatcagc gtgccgaagg
     7201 caggcatgta tgcgccactc tctaggcggg caatctgagg ttgtttcatg ccggtcatac
     7261 gggcgagttg cgattgactc agcccgcgtt gttcgcgaag tttggccaat tccacggcaa
     7321 tcgtaacctc tcgctcagcc tcggcgagat cctgcgcaaa ggccggtttc ttctcaatgc
     7381 gttctttgat gtactggcga tgggttttca tggtcatacc tctttaacac gtcggcatat
     7441 cgtttttcgg ctagggcaat atctgattcc ttgagccgct gtgtcttctt ctggacggca
     7501 tggaggatga caaactgttg ccctacgatg gccccataaa agaaccgata ctcattccca
     7561 gaccattccg gccgtaactc ccaaatcttt ccccgtacat gcttagcgat tgaggcggga
     7621 agttgtgttc ccctcgtttc aagcagggtg aggtaggcca agcattttgc cctggctttg
     7681 tccgactgtt tatccaggaa ggtttctatc ggggtgcccc cgtcacccgt ctcatagtaa
     7741 acgactgtcc agggcatgac ggaaatataa cactcatgtt atagcacgtc aaatgaacag
     7801 gcgggccatg tttgcggcta tgatctcacc atggaaatgc gggccgaata aggctagggc
     7861 gtgtcacaat tggggaccgt tcatgccctc gaagaggcgg gccagatggg agcggtcacg
     7921 ctccagggtc ctagtcggcc acaggctcaa gccggtaggt gtgtgggcac gattccgcca
     7981 gcggtagagg ttgtgaaggc ggttgttatg attatgattc gcccggacgg taaagggcgg
     8041 gcctttagta cgtagacacc ctgttgtgaa aagcgttggg aaatgagtgg ggaagagaaa
     8101 ggaattcagg aggcggttct gccgttgaat gtagcctcag gtctacactg ggtctacatg
     8161 tttatttttc ttggcggtga atgtctcgta agtgattgat tctgtggtgc gcccgacagg
     8221 acttgaacct gtagccttct gatccgtagt cagatgctct atccattgag ctacgggcgc
     8281 gtattctatt tattgagccc gttcgaagag cggtcccttc ggagcgggct caaaggagca
     8341 gcgcccgtta tacaggcttg agggaagaac ggtcaagtct gtcgagcata catatttttt
     8401 agaagagac
//
LOCUS       DAORRQ010000110         8051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_366_length_8051_cov_109.354552, whole genome shotgun sequence.
ACCESSION   DAORRQ010000110 DAORRQ010000000
VERSION     DAORRQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_366_length_8051_cov_109.354552"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PK224_19585"
     CDS             <1..490
                     /locus_tag="PK224_19585"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HRB18007.1"
                     /translation="AETAVGHYPVEVVQVMDRIIRAAEVETGPCFVRRSQGEQGQDSI
                     PEAISLSAYSAAATIGASAIVAFSERGTTARLVSKQRPVAPIIGLTPFETVRRQMALY
                     WGVLPHTVPQIPTTDERVNEAERRLKAEGLARSGQRIVILSGTRIGQPGGTNLMKLHE
                     VA"
     gene            495..1058
                     /locus_tag="PK224_19590"
     CDS             495..1058
                     /locus_tag="PK224_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18008.1"
                     /translation="MNSPRRSVLIMFIAAVLLLPASVTPGLAQTNAVSHSPAKVVEKY
                     FALDNKGVRLDAGSFEAVAGLVDWKEEPAWGKVIVINGFSVPDDFRQWEIVNRLEVLV
                     PVEFHVLGVMYLDTAGFVPEPGTEQVRVRVKVQGARWKIMEPILPPHVGQKRMLNVVR
                     QAMLEEKDGVRHESLAALQAELRKARE"
     gene            1055..1705
                     /locus_tag="PK224_19595"
     CDS             1055..1705
                     /locus_tag="PK224_19595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRB18009.1"
                     /translation="MTKKAEIDLLIFDLDGTLIESKWDIADSVNLTLRDLGVPERPQE
                     EIFGFVGDGVKKLLRLAVGEGNKDLYDEALRVFRGHYLEHCLDRTSFYPGIDRVLTHF
                     STKPKAVATNKAIEYTNVILKGLGPQHFVYVVGGDNGFGLKPEPGMLVHVMEQLGVEK
                     DRTVLVGDSTNDINGGHNAGIRVCAVGYGMGNRQKMAACEPDWFIERPEELMELFI"
     gene            1702..3273
                     /locus_tag="PK224_19600"
     CDS             1702..3273
                     /locus_tag="PK224_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB18010.1"
                     /translation="MISITGARTMGALILVGVLAAAVPGQAGSPSLADRVIEHKLANG
                     MTVLMVERHQAPIVSVNMTFGVGGVNEQVGQTGLAHLYEHMAFKGTRTVGTRDYEREQ
                     AVLDDLAMVGTELDRRERDEAARAQAEGKPPVPSERVQQLQRRFKELQEKAGEYVVGN
                     EMALLYQRHGGVGLNASTGKDITRYVISLPANRLPLWAALESDRMAHPVLREFYKERG
                     VVMEERRLRTDDSPNGLLYETFTSTAFQAHQYGVPTIGWGSDILSLTPAATEAFFKTY
                     YGPNNATVAIVGDINPKEVIALIEQTFGKIPAAPPIPSLVTEEPPQRGERRVEIEFDA
                     EPALAIGYHKPTIGHPDDFVFDVIDEVLTEGVTSRLYSTLVRDKRLAASVLSDTNYPG
                     VRAPNLFVIAATPLAPHTTPEVESAIYEELDRLKAEPISTKEFERVLNGLDADLVRSL
                     RSNSGLASQLAFYQTVAGTWRYVLNARDRIAAVTPADVQRVAAQYLTKPNRTVGVLVK
                     KAQDKKMAAAGEVAR"
     gene            3342..4778
                     /locus_tag="PK224_19605"
     CDS             3342..4778
                     /locus_tag="PK224_19605"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRB18011.1"
                     /translation="MGRMSCFAMVLMLLLQATVGLAADPTSNDPRTMRFPTVEFNPPD
                     AERVVLENGMVVYLLEDHELPLVTINATLRTGSWLDPADKVGLAGMTGAVMRTGGSAG
                     MSPDEVDEELEQLAASMSIGFAKESGSASLDVLKKDLKRGLQIFADLLRRPAFEQGRV
                     ELAKLQALEGIRRRQDSPGSIVGREFAKLLYGPTHPSARETSVRSIEAITREDLVAFH
                     QRTVHPNGIILGVTGDFEKADMLALLRAAFGDWAKGNVPAVTIPAVSEPDAKTGLVRF
                     VNKDTSQTHLRVGHLTIKETDPDYVAVAIANDILGGSSFRSRLFNDVRTKRGLAYSVG
                     SGLRASVYDEGVWLMRAETKLSSTQEVVNRFVANMERMRNEPVTDTELEEAKEAYVNS
                     FVFSFTSASSIVGRLIDLEYDGLPKDWLQQIRDRVVKLTKEDIQRAAKAHFNPERLRI
                     LAVGSGEALSKVLASFGEVKEITLVPES"
     gene            4812..5648
                     /locus_tag="PK224_19610"
     CDS             4812..5648
                     /locus_tag="PK224_19610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB18012.1"
                     /translation="MPLEDELSDILKKARNGQQRSMADVARASGLSEVEVSELERGQS
                     PRSREQVQAVARALGLRAEPLTQVVDGWTPEALPSSVPHVETVFGSIGGYEVKGYVVH
                     DRGEAVVVDTAYNASGMLALLAQRKLRLRAICLTHGHSDHAEGIEAILKAYPVPVYLG
                     PDDFDLLSWRPSPQVLHVPQGGETLPVGGLTIRFMPTPGHTPGGVCYRLEQAGAPICF
                     VGDTLFAGSIGRSNPAGLYETHLESVRKQVLTLAPDTRLFPGHGPATTVREELLHNPF
                     YS"
     gene            5868..6827
                     /locus_tag="PK224_19615"
     CDS             5868..6827
                     /locus_tag="PK224_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRB18013.1"
                     /translation="MNRDDSSEQPRSPDEPISLSSDESDEDEWEEERPQFSAWMLPAL
                     LFLLTVFTVLWAGAYQTNTNPLVGPWNFLVDDPGSLWRGVPFAATLLGILVTHELGHY
                     VLSRIHGVPTSLPLFVPGLPHFVGTFGAIIRMRAPLTDRRALFDIGVAGPIAGFVVAV
                     IALVVGLRLSTVVPIQTSYGMHLGEPLLLQFASWVVIGPLAPTADVILHPIGFAAWFG
                     LFITSLNLLPIGQLDGGHVAYALLGDRQRSVAVALVPILMVFGWLGWKGWFLWVGLAG
                     MMGLAHPPVQNPHRELGGIRVLVGWIALAIFVLTFSWEPFILR"
     gene            6831..7559
                     /locus_tag="PK224_19620"
     CDS             6831..7559
                     /locus_tag="PK224_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18014.1"
                     /translation="MQCPKCGWEQGDEQVECARCGVIFAKLANAPRPVPATRPRLPVQ
                     DSAWFRLAEDRLLTTEESVNPFHFTGRVLAFLVLVLWGWRFMTTPLETNYTGESFLHL
                     VNLPFHEAGHLLFMPFGRFMTILGGSLGQILMPLVCLGTFLLKTRDPFGGSVALWWTA
                     ENFMDVAPYINDARAMDLLLLGGFTGKEVDAHDWNNLLTMLGWLQYDHGLAKLSYGMG
                     TVLMLLALAWGAMLLHRQYRRLDW"
     gene            7622..>8051
                     /locus_tag="PK224_19625"
     CDS             7622..>8051
                     /locus_tag="PK224_19625"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRB18015.1"
                     /translation="MRTLASLCRIAAVPILLGVGLMAGQVHADEGASRFVLMLDGEAV
                     KDSASGLVWEREPDYLFDVWDRSVARCPTKTVGGQQGWRAPSIDEIKTLVDPDQQDPS
                     LPAGHPFRNIRSGIYWTATPHPKDDIVAWQQSFFSGQAVTD"
BASE COUNT         1465 a         2264 c         2671 g         1651 t
ORIGIN      
        1 cgcggaaacg gccgtcggcc attacccggt ggaagtcgtg caggtgatgg atcggatcat
       61 acgggcggcc gaagtcgaaa cggggccctg ttttgtccgt cgctcgcagg gcgagcaggg
      121 acaggattct attccggaag ccatctcgct ctcggcctac tcggctgccg cgacgatcgg
      181 cgcgagtgcg attgtcgcct tcagtgaacg gggaacgacg gcccggctgg tctccaagca
      241 acgtccggtt gcgccgatta tcgggttgac gccgttcgag acggtccgcc ggcagatggc
      301 gttgtattgg ggggtgcttc cccacaccgt gccacagatt ccgacgaccg atgagcgggt
      361 caacgaggct gagcggcgct tgaaggccga gggtttggca cggagcggac aacgcatcgt
      421 cattttgtcc ggcaccagaa tcgggcaacc gggcgggacg aatctaatga aattgcatga
      481 ggtggcttag tcgcatgaac agtccacggc gcagcgtgct catcatgttc atagcggcgg
      541 ttcttctgtt gccggcctct gtgactccgg gcctggctca aaccaatgcg gtgtcgcatt
      601 ctccggcgaa ggtggtggag aaatactttg ccctcgacaa caagggggtg cggctcgatg
      661 ccggttcctt cgaggcggtg gcgggcctcg tggattggaa ggaagagccg gcctggggca
      721 aagtcatcgt catcaacggc ttttcggtgc cggatgattt tcgccagtgg gagattgtga
      781 acaggttgga ggtattggtg ccggtggagt ttcatgtgct gggcgtcatg tacttggata
      841 cggccggctt cgtaccggaa ccgggcacgg agcaggtgcg tgtccgcgtg aaggtgcagg
      901 gggcacgatg gaaaatcatg gagccgattc tcccgcccca tgtcggccag aagcggatgc
      961 tgaatgtggt ccggcaggcc atgctggaag agaaggacgg agtgcgccac gagtcgttgg
     1021 cggccttgca ggccgagttg cgaaaggcac gagaatgacg aagaaggctg aaatcgattt
     1081 gctgattttc gacctcgacg gcacgttgat cgagtcgaaa tgggatatcg cggacagcgt
     1141 caatttgacg ttgcgcgatc tgggtgtgcc ggagcgcccg caggaggaaa ttttcggatt
     1201 cgtcggcgac ggcgtgaaga agctcttgcg gctggcggtc ggcgaaggca acaaggactt
     1261 gtacgatgaa gcgctccggg tctttcgcgg acattatctg gaacattgcc tggatcgcac
     1321 ctcgttttat ccggggatcg accgggttct cacccatttc tccaccaagc ccaaggccgt
     1381 ggcgaccaat aaggcgatcg aatataccaa cgtgattttg aagggcctcg gcccgcaaca
     1441 ctttgtctat gtggtcggcg gcgacaacgg gttcggtctc aagcccgagc ccggcatgct
     1501 ggtgcatgtg atggaacaac tcggagtcga gaaggaccgt acggtgctgg tcggcgacag
     1561 caccaacgac atcaacggcg gtcacaatgc aggcatccgg gtctgtgcgg tggggtatgg
     1621 catgggcaac cgtcagaaga tggccgcctg tgagcccgat tggtttattg aacgaccgga
     1681 ggaactgatg gagcttttca tatgatcagc atcacagggg cgagaacaat gggtgcgctt
     1741 atcctggtcg gagtgcttgc ggcagccgtg ccgggtcagg ccgggtcccc gagtctggct
     1801 gaccgggtca tcgagcacaa gcttgccaac gggatgactg tgttgatggt ggaacggcac
     1861 caggccccga tcgtcagcgt caacatgacg ttcggagtcg gcggggtgaa cgagcaggtc
     1921 ggccagaccg gcctggcgca tctgtacgaa catatggcgt tcaagggcac ccggacggtc
     1981 ggcacgcggg attacgagcg ggaacaagcc gtcctggacg atttggccat ggtcggcacc
     2041 gagcttgacc ggcgtgaacg tgacgaagcg gcccgcgcgc aggcggaagg caaaccgcct
     2101 gttccctccg agagggttca gcagctacag cggcgtttca aagagctgca ggagaaggca
     2161 ggggagtatg tggtgggcaa tgaaatggcg ctgctctatc agcgccacgg cggcgtgggt
     2221 ctcaatgcgt cgaccggcaa agacatcaca cgctacgtca tcagtttgcc ggccaaccgg
     2281 ttgccgctct gggcggcgct ggagtcggac cggatggcgc atccggtcct gcgcgagttt
     2341 tataaggaac ggggcgtggt catggaggag cggcggctcc ggaccgacga cagtccgaac
     2401 ggcctgctct acgaaacgtt cacttccacg gcgttccagg cgcatcaata cggtgtgccg
     2461 actatcggct gggggtcgga catcctgtcc ctcacgccgg cggcgaccga ggcgttcttc
     2521 aagacctatt acggaccgaa caatgcgacg gtggcgattg tcggggacat caatccgaaa
     2581 gaagtcattg cgttgatcga gcagaccttc ggaaaaattc cggccgcgcc tcccattccg
     2641 tccctggtga cggaggagcc gccgcaacgc ggtgagcgac gcgtcgaaat cgaattcgat
     2701 gccgagcccg cgttggcgat cggctaccac aagcccacca tcggccatcc cgacgatttt
     2761 gtattcgacg tgatcgacga agtgttgacc gaaggcgtga cgtcgcggct ctacagcaca
     2821 ctcgtgcgtg acaagcggct ggcggcttcg gtgttgtccg atacgaatta tcccggcgtc
     2881 cgcgccccga acctgttcgt gatcgcggcg actccgctcg ctcctcatac cacgccggag
     2941 gtggagtcgg cgatttacga ggaactcgac cggttgaagg ccgagccgat ttcgaccaag
     3001 gagttcgagc gggtgctcaa tggactcgat gcggatctgg tgcgatccct gcgttcgaac
     3061 agcggcctcg cctcgcagtt ggccttttat cagactgtgg ccggtacctg gcggtatgtg
     3121 ctgaacgcgc gtgaccggat cgcggcggtg accccggccg atgtgcagcg ggtggctgcc
     3181 cagtatttga cgaagccgaa ccggacggtc ggtgtcctgg tcaagaaggc gcaagacaag
     3241 aaaatggcgg ctgcgggtga ggtggcccga tgaggcgcgt cgttcgtgaa gcgtctctcg
     3301 tgaagcgcga aacggagaga ggaattccca tgaggaaagc gatgggacgg atgtcgtgtt
     3361 ttgccatggt gctcatgctc ctgttgcaag cgacggtagg attggcggcg gacccgacgt
     3421 cgaacgatcc tcgcacgatg cgattcccga ccgtggaatt taacccgccg gatgcggagc
     3481 gtgtggtgct ggaaaacggc atggtggtct atctgctcga agatcacgaa ctcccgctgg
     3541 tgacgatcaa tgcgacgctg agaaccggca gctggctgga cccggcggac aaggttggcc
     3601 tggccggtat gaccggggcg gtgatgcgca cgggcggttc ggcgggaatg tcgcccgatg
     3661 aggtcgatga agagctcgaa caattggccg cttcgatgtc catcggattt gcgaaagaat
     3721 ccggctcggc ctctctcgat gtgctgaaga aagatctgaa gcgcgggctg cagatcttcg
     3781 ccgatctgtt gcgtcggccg gcgttcgagc agggccgggt ggagttagcg aaattgcagg
     3841 cgttggaagg catcaggcgg cggcaggaca gtcccggctc gatcgtcggg cgggagtttg
     3901 ccaagctgct ctacggcccg acccatccga gcgcccgtga aacgtcggtt cgttcgatcg
     3961 aggcgattac ccgtgaagat ctggtggcct ttcatcagcg gaccgttcat cccaacggca
     4021 tcatcctcgg cgtgaccggc gatttcgaga aggccgacat gctggccctg ttgcgtgccg
     4081 ccttcggcga ttgggccaag ggcaacgtac ccgctgtcac gattcccgct gtgtccgagc
     4141 ccgatgccaa gaccggtctc gtacggttcg tgaacaagga cacgtcgcaa acgcacctgc
     4201 gggtgggaca tctgaccatc aaggagaccg atcccgacta cgtcgcggtc gcgatcgcca
     4261 acgacatcct gggcggcagt tcgttccgca gccggctctt caacgacgtg cgcacgaaac
     4321 gcggcttggc ctattccgtc ggcagcggcc tgcgggccag tgtgtacgat gaaggcgtct
     4381 ggctgatgcg cgccgaaacc aagctgtctt cgactcagga agtggtgaat cgtttcgtgg
     4441 ccaacatgga gcggatgcgc aatgagccgg tgaccgacac cgaattggaa gaagcgaagg
     4501 aggcctatgt gaactcgttc gtgttttcct tcaccagcgc gtcgagcatt gtggggcgtt
     4561 tgatcgatct tgaatatgac gggctgccga aagattggct gcagcagatc cgcgacaggg
     4621 tggtgaagct gacgaaagag gatattcagc gggcggcgaa ggcccatttt aatcccgagc
     4681 ggctgcggat tctggccgtc gggtccggtg aggccctgtc gaaagtgctg gccagtttcg
     4741 gtgaggtgaa ggaaattacg ctggtcccgg agagttaagc gcgttcgtgg gttcttaggc
     4801 cggagactcc tatgcctctt gaagacgaac tcagcgatat tctgaagaag gctcgcaacg
     4861 gtcagcagcg gtcgatggct gatgtggcgc gtgcgagtgg cttgtctgaa gtcgaagtga
     4921 gtgagctgga acgggggcaa tcgccacgga gtcgcgaaca ggtccaggct gttgcccggg
     4981 cgcttggact gagagcggaa ccgctgacac aggtggtgga cggttggact cctgaggcct
     5041 tgccttcctc ggtgccacat gtggagaccg tgtttgggtc gatcggcggg tatgaggtca
     5101 agggctacgt cgttcatgat cgcggagaag ccgtcgtcgt cgatacggcc tacaatgcga
     5161 gcgggatgtt ggcgctgttg gcccagcgga agcttcggtt gcgggccatc tgtctgacgc
     5221 acggtcactc cgatcatgcc gagggcattg aggcaatcct caaggcatac ccggtgccgg
     5281 tctaccttgg ccctgacgat ttcgatctct tgagctggcg gccatcgccg caggtgttgc
     5341 atgtgccgca gggaggcgag acgctgccgg tcggcgggct gaccatccgg ttcatgccca
     5401 caccgggcca tacaccgggc ggggtctgtt accgtcttga gcaggccggt gccccgatct
     5461 gttttgtggg ggatacgctg tttgccggct ccatcggccg ctccaatcca gccgggctct
     5521 atgagaccca tctggaatcg gtccgcaagc aggtgttgac gctggctccc gacacaaggc
     5581 ttttcccggg acatggaccg gctactacgg ttcgtgaaga gttactccac aacccgttct
     5641 actcctagca gggtgcggaa caactcgatt gttgcgcaat acgttatgat caactcgtgc
     5701 gtcgtgtcgg tatcagaatc acccgcagga tgcgcaaaaa ggccgtccag caaggccgca
     5761 gcgagcgaag gggcgaatcg tactcgtgca gtacggtgag tcgcttcgcg atgcgagaac
     5821 gccgccggcg gtctttttcc gcatcctgct aggactggag gccgggcatg aaccgtgacg
     5881 actcgtcgga gcagccgcgg tctccggacg aaccgatttc cctttcgtcc gacgagagcg
     5941 acgaggacga atgggaggag gaacgtccgc agttctctgc atggatgctt ccggcgctcc
     6001 tgttcctgct taccgtcttt accgtcctct gggccggggc ctaccaaacc aacaccaatc
     6061 cactcgtcgg cccgtggaat tttctcgtcg atgatcccgg ctcgttatgg cggggtgttc
     6121 catttgccgc caccctgctg ggtatcctgg tgacgcatga gctgggtcac tatgtgttgt
     6181 cgcgcatcca tggcgtgccg acatcgctgc cgctctttgt gccggggttg ccgcatttcg
     6241 tcggcacgtt cggtgccatt atccgcatgc gggcaccttt gacggatcgc cgggccctgt
     6301 tcgatatcgg cgtggcgggg ccaatcgccg ggtttgtggt ggcggtgatc gcgctggtgg
     6361 tgggccttcg gctctcgacg gtggtgccga ttcaaaccag ttatggcatg catctcggcg
     6421 agccgctcct ccttcagttt gcgtcgtggg tggtgatcgg tccgctcgct cccaccgcgg
     6481 atgtgatcct tcacccgatt gggtttgccg cctggttcgg gctcttcatc acctcgctca
     6541 acctgcttcc gatcggtcag ctcgacgggg gacatgtggc ctatgcgtta ttaggagatc
     6601 ggcaacgcag cgtggcggtc gccctcgttc cgatcctcat ggtcttcggc tggttgggct
     6661 ggaagggttg gttcttgtgg gtggggcttg ccggcatgat gggactggcc catccaccgg
     6721 tgcagaatcc ccatcgtgaa ctagggggaa tccgtgtgct ggtgggttgg attgccctgg
     6781 cgatctttgt cctgaccttc tcctgggagc cgtttatttt gcggtagccg atgcagtgtc
     6841 caaagtgcgg atgggaacag ggcgacgaac aggttgagtg tgctcgatgc ggagtgattt
     6901 tcgcgaagct cgccaacgcg ccacggcccg ttcccgccac cagaccgcgc ctaccggtcc
     6961 aggactcagc ctggttcaga ctcgccgagg accggctgct cacgacggag gagtccgtca
     7021 atccgttcca cttcaccggg cgggttctgg cgtttctcgt gctcgtcctg tggggctggc
     7081 gcttcatgac cacgccgctc gagacgaatt acacgggcga atcgtttttg catttggtca
     7141 atctaccgtt tcatgaagcg ggccatctgc tcttcatgcc cttcgggcga ttcatgacca
     7201 tcctcggcgg cagtctggga cagatcctca tgccgctggt gtgtctgggc acctttctcc
     7261 tcaagacccg cgatccgttc ggtggatcag tcgccctgtg gtggacggcc gaaaacttca
     7321 tggacgtcgc gccctacatc aacgatgctc gcgcgatgga cctcctgttg ctcggcgggt
     7381 tcaccggcaa ggaagtcgat gcgcacgact ggaacaatct cctcaccatg ctcggctggt
     7441 tgcagtatga ccatggtctg gcgaagctgt cgtacggcat ggggacggtg ttgatgctgc
     7501 ttgcgctcgc ctggggcgcg atgctcctgc atcgacaata ccgacgactg gactggtaac
     7561 cgccttcgac tcatctggct ctccttgaaa agacccggca catcgggcac actgccggac
     7621 tatgcgtacc ttggcgtcgc tctgccgcat cgctgctgtt ccgatcttgc ttggcgtcgg
     7681 cctcatggcc ggtcaggtcc atgccgacga gggcgcgtcg cggttcgtct tgatgctgga
     7741 cggcgaagcg gtgaaagatt cggcgagcgg gctggtgtgg gagcgcgaac cggattacct
     7801 gtttgacgta tgggatcgct cggtcgcccg atgccccacg aaaaccgtcg gcgggcagca
     7861 gggctggcgt gccccaagta tcgacgaaat caaaacgctc gtcgatcccg atcagcaaga
     7921 tccttcgttg cccgcgggtc atccatttcg caatatccgg tccggcatct actggacggc
     7981 cacaccccat cccaaggacg acatcgtggc ctggcagcaa agtttcttct ccggccaggc
     8041 cgtgaccgat c
//
LOCUS       DAORRQ010000111         7954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_371_length_7954_cov_49.362831, whole genome shotgun sequence.
ACCESSION   DAORRQ010000111 DAORRQ010000000
VERSION     DAORRQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_371_length_7954_cov_49.362831"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..946)
                     /locus_tag="PK224_19630"
     CDS             complement(134..946)
                     /locus_tag="PK224_19630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRB18016.1"
                     /translation="MMVPKQQGKQWMMRVPVASWVVLVMVLSCWSAADAAQRFVDLTY
                     TFDETTQVWPANPPFHRDSTEQGGTPTEKWYATGQVALSEHAGTHMDAPVHFAQGQAG
                     IDGIPVDRLIGPAVVIDVRVAVKEDRDYRVSLSDIHRWESAHRTIPAGAIVMLLTGWG
                     VYWTDRERYFGSATPDVPTTLHFPGFSQEVAEFLVAERHISGIGIDTASIDYGPSRDF
                     VVHRIVNGAGLYGLENVARLDEVPASGATVMALPMKIAGGTGAPVRIIAILP"
     gene            1064..2233
                     /locus_tag="PK224_19635"
     CDS             1064..2233
                     /locus_tag="PK224_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HRB18017.1"
                     /translation="MPNGSSRSIDLRFSGERFSVEYRLHGSASYARRRADLLCIDQTV
                     EAADHVIPSGAIRDQLLGRVIQLEALTPESHRAVVSFPIELLDGTMSALLHMIIGMAG
                     LQGQIRVTDLSLPDAVVSRLTGPRFGSRGLRDLLRVPRRPLICAVLKPLGYSPQQLAE
                     LAHEFALGEVDLIKDDQSLRDHPFCRFEERVARCAEAVAKASRQTGKRCLYAPHVSGP
                     WPSLMEQAAKATQAGAGALLLCPGLTGFDAMASLAQLSSVSLPLIAHPDFLGSHYVTS
                     DSGIAHALLFGLLPRLAGADITIYPTYGLNYPISEQDCRQIATACRRTLGSCPPILPT
                     AAGRMTASRIREMVTLYGSDLVFILGSDLRREPTHLRSACLDFLRLVEAETSPSP"
     gene            2490..3836
                     /locus_tag="PK224_19640"
     CDS             2490..3836
                     /locus_tag="PK224_19640"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HRB18018.1"
                     /translation="MALLRTSDEISKLDTSDTLVLRPEHLRLGMYVDLNCSWFKHPFP
                     RRSFKLTSQNQITTIKGLGLATVLVYPRESDPETDEEGNAILPPPSSTGVSETTAQTS
                     AAEAIAEPEAPTPAEEEQPTHGDYHQAVELASTAYRQMLDQSSQMMKNLVSGSEEGLR
                     HAQSMIGGLSALLTNTEAAGTVASAFDSEDVTNANQLHALNVATLSMIVAQQFDIDQE
                     ALQLIGMAGLLLDVGEQRIPRHILQTRARRSEVENVKYQLHPYLGVEMLRRFPGFPEE
                     VLDVIRSHHERLDGSGYPEKLKGDQISLPTRIVSAVDHYDALVNSFHPEDALSPAEAL
                     ATMYKHQQRLFSSEVLIAMIQTLGVFPPGTVVTLTDGSFGQVLNINFKYRLKPLVLLH
                     DQAAPADQPRTVDLQTESELSILRAMPRDELPGNVVEYFRIKRWTGYFIQSSMRAA"
     gene            3900..6092
                     /locus_tag="PK224_19645"
     CDS             3900..6092
                     /locus_tag="PK224_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB18019.1"
                     /translation="MAKPRVLLVIPPLTQLNTPYPATAYLTGFLRSRGYETAQADLGL
                     EMVLRLFSRSGLEQAFTRIKQTERPWRGEIQQILTLETAYLQTIEPVIAFLQGKDPML
                     APHLARPGFLPEGPRFAAHRRPGEGSQASSPSDTARHRATLYIEDLTDLLHSTVAPHL
                     FLNRYAEQIMQATSSFDRIEDALAQPLSLTDELLLEALWPHLHRTTPDVVGLTIPFPG
                     NLFGALRIAQAIKAQSPPTVIVLGGGYVNTELRRLEDPRLFQYVDYVTLDDGERPFLC
                     LLEHLAGGRPEPLLRRTFLCRDGRVIFRDGSNEPDISMHELGRPTYAGLPLTDYLSIL
                     DSLNPMHRLWSEGHWNKLTVAHGCYWKQCTFCDVGLNYISRYEVTPTDVLIDQIEALI
                     QETGQRGFHFVDEAAPPAALKALALRLLETGLTISWWGNIRFEEAFSPDLARLLAASG
                     CIALTAGLEAASDRLLERMKKGITVDQTARVAAAFRQAGILVHAYLMYGCPGETIADT
                     VDSLERVRQLFANHLLQSAFWHKFTATAHSPIGLNPAAEGLTILGPTFEGFAENDLRH
                     ADPNATCPPWLGEGLRAALLNYMEQAGFDIPSQRWFSHRVKPPRVTKTWLTQALAASS
                     DVPLTAERRCVWIGGRPVIAATGRTRTRVILPTRTEDFTISLASPHTEWLLRFLHEST
                     PSLSRRNHAYPRLAEVRDRFPAAGARGFDRFSRGSAWQSMRQAGLLLV"
     gene            6354..7184
                     /locus_tag="PK224_19650"
     CDS             6354..7184
                     /locus_tag="PK224_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRB18020.1"
                     /translation="MLITRLLTPLLATGILLSAPLSALAIAVYSSSGPNAASIQGSVN
                     GFRSDLGTNNGVGGSFASGRREINWDGVPAGFSDPNNLPGNFFNSNSARGAVFETPGS
                     AFKVSANSGVGTPERFGSINPSFPNSFSVFSSQKLFTAIDSNIVDVRFFVPGTNQPGA
                     VRGFGSVFTDVDLAGPTTIEFFDFHNNSLGKQTVLNTPGVTSLSFLGVSLEQAIINRV
                     RITAGNAALSALDGFDFVAMDDFIYGEPQAVPAPAAVLLLGSGLAALVWARRKSLLPE
                     "
     gene            complement(7350..>7954)
                     /locus_tag="PK224_19655"
     CDS             complement(7350..>7954)
                     /locus_tag="PK224_19655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRB18021.1"
                     /translation="FTAETTEGTINFHEWLGGGWGILFSHPKDYTPVCTTELGYMARI
                     KGEFDKRGVKILAISVDPLDSHRGWTKDINETQNCTVSYPIIADPEKKVADLYDMIHP
                     NSLDSMTVRSVFVIGPDKKIKLMLTYPASCGRNFDELLRVVDSLQLTAKYKVATPVNW
                     KDGQDCIITPAVNDAEAKTLFPKGFKSIKPYLRLTPQPNK"
BASE COUNT         1636 a         2579 c         2089 g         1650 t
ORIGIN      
        1 ggaagcccgc cgaggcttct cgcacgtaag gccgacgcag gcgtacttgc agtccgtcga
       61 ggaggccgaa cgagaaaagc ttcgccgggc aataggatcg gacgacggag caggtatcca
      121 tgaatagtcc gggctaaggc agaatcgcga tgatacgaac cggagcccct gttccaccgg
      181 caattttcat cgggagagcc atgaccgtgg caccggacgc cggcacctca tccagtcgcg
      241 ccacattctc gagtccatag aggccggctc cgttcacaat ccggtgcacc acaaaatcac
      301 gggaaggacc ataatcgatg ctggctgtat cgattccgat tccggagatg tgtcgttccg
      361 ccaccagaaa ctccgcgact tcttgtgaga agcccggaaa atgcagcgtc gtcggcacat
      421 ccggcgtggc gctgccgaaa taccgttcac gatccgtcca gtacacaccc cagcccgtca
      481 gcagcatcac gatcgctccc gctggaatcg tcctgtgcgc agactcccat cgatgtatat
      541 ccgacaagga gacgcgatag tcgcgatctt ccttcaccgc aacgcgcaca tcgatgacca
      601 ccgcagggcc gatcaggcgg tcgaccggaa tgccatcgat tcccgcttgc ccctgtgcga
      661 aatgaacggg cgcatccatg tgcgttccgg catgctccga cagcgccacc tgccctgtgg
      721 cataccattt ttccgtcggc gtacctccct gttcggtcga gtcacgatga aagggaggat
      781 tggcgggcca gacttgcgtg gtctcatcga acgtgtaggt caggtccacg aaccgttgcg
      841 cagcgtcggc ggcactccag caactcaaca ccatcactag gaccacccag ctcgcaaccg
      901 gaactcgcat catccactgc ttgccttgtt gcttcggcac catcatccac gctccaacaa
      961 tcggcctcgt acgagaacct gctcagaacg ccccacctat cggatcaaac ggaaatacgg
     1021 taccctctcc tcctcgaaca gtgcaaccaa gaagagagca gacatgccca acggttcaag
     1081 ccggagtatc gacctccggt tttcaggaga gcgcttttca gtggagtatc gtcttcatgg
     1141 atcggcctcc tatgcgcgca ggcgggcaga cctgctctgt atcgaccaga ccgttgaagc
     1201 cgccgatcat gtcattccct ctggagcgat tcgcgatcag cttctgggcc gagtgattca
     1261 gctcgaggcg ctcacccctg aatctcaccg cgcagtagtc agtttcccca ttgagctcct
     1321 cgacgggacc atgagcgctc tccttcacat gatcatcggc atggccggac tgcaaggaca
     1381 gatccgcgtc acggacctct cattgcccga tgcggtcgtc tcccgattga cggggcctcg
     1441 attcggctcg cgcggcctcc gcgacctact gcgggtcccc cggcgtccgt taatctgcgc
     1501 ggtgttgaag ccgctcgggt attctccgca gcagctggct gaactggccc acgaatttgc
     1561 cctgggtgag gttgatctga tcaaggacga ccagagtctg cgcgatcacc ccttctgtcg
     1621 cttcgaggag cgggtcgcac gttgcgccga agccgtcgcc aaagcctcgc gccagaccgg
     1681 aaaacggtgt ctctatgcgc cgcatgtctc cggcccctgg ccctctctca tggaacaggc
     1741 cgccaaggca acgcaagcgg gcgcgggcgc actcctgctc tgtcccgggt tgacaggctt
     1801 tgacgccatg gcttccctgg ctcagctgtc atcggtgtcg ctgcccctca tcgcacatcc
     1861 ggactttctc gggagccact atgtgacgtc ggacagcggc atcgctcacg cgcttctctt
     1921 cggccttctc ccgagactgg ccggcgccga tatcaccatt tacccaacct atggcctgaa
     1981 ctatccgata tcggaacagg attgtcgtca gattgccacg gcgtgccgac gaacgctcgg
     2041 cagctgccca cccatcctcc cgaccgccgc aggaagaatg accgcgtcgc gtattcgcga
     2101 gatggtcacc ctctacggtt cggacctcgt cttcattctt ggcagcgacc tacgacgaga
     2161 gccgacacac ctccgctctg cctgcctgga ttttcttcgt cttgtcgaag cggagacatc
     2221 cccatcccct tgatgctgct tctcatgctg aaggcaatat ctatcttata ctattgatat
     2281 tactggcata attgagtcct ctccatctaa atacgatgca gatccaggaa ccggatccga
     2341 ccttcgccga aaataggacg actcgcaata cgtggagaga caccagccat cccctttctt
     2401 tatcaagttt cctctccccc tgtccgatat agcccatagc ctgaagtgct tacgtatcga
     2461 ccgaccgttc ccgtgcaaaa gcaacaccca tggcgctgct tcgaacctca gacgagatat
     2521 ctaaactcga cacgtccgat acgttggtgc tccgtccgga gcatcttcgt ctgggcatgt
     2581 atgtcgacct gaattgctcc tggttcaagc atcccttccc gcgccggtcg ttcaaactca
     2641 cctcccaaaa ccagatcacg acaatcaaag gactcggatt ggccaccgtg ttggtctacc
     2701 cgcgggaatc ggacccggag acggatgaag agggcaacgc tatcctgccg cctccgtctt
     2761 cgacgggcgt ttcggaaacg actgcacaga ccagcgcagc cgaggccatc gccgaaccgg
     2821 aggctccgac cccggccgaa gaagagcagc cgacccatgg cgactaccat caggcagtag
     2881 agctagcctc caccgcctat cggcagatgc tcgatcagag ttcgcagatg atgaaaaacc
     2941 tcgtgagcgg ttcagaggag ggattgcggc acgcgcagtc catgatcggc gggctcagcg
     3001 cgctcttgac caacactgaa gccgccggca cggtcgcgag tgccttcgac tcggaagatg
     3061 tcaccaacgc caaccaactg catgccctca atgtggcgac gctttccatg atcgtggcgc
     3121 agcaattcga catcgatcag gaggcgctcc aattgatcgg catggccgga ctgctcctcg
     3181 acgtggggga acagcgcatt ccgcgtcaca tcttgcagac ccgtgcccgc cgttcagaag
     3241 tcgagaatgt gaaatatcag ctgcatccct atcttggcgt ggagatgctc agacgattcc
     3301 cggggtttcc cgaggaagtg ctggacgtca tccgcagcca tcatgaacgg ctggacgggt
     3361 ccggctaccc cgagaaactc aagggagacc aaatctcgct tcctaccaga atcgtcagcg
     3421 ccgtggacca ctacgacgca ctcgtcaata gctttcatcc tgaagacgcc ctttccccgg
     3481 cggaagcgtt ggcaacgatg tacaagcatc aacagcgact cttttcctcg gaagtcctga
     3541 tcgcgatgat ccaaacgctg ggcgtgtttc cgcccggaac cgtcgtgacg ctgaccgacg
     3601 gctcgttcgg gcaggtgctt aatatcaatt tcaaataccg tctcaagccc ctcgtgctgc
     3661 tgcacgacca ggccgccccg gccgaccagc cccgcaccgt agatctccaa acagagtcgg
     3721 agctcagcat tcttcgggcc atgccgcgtg acgagttgcc agggaatgtg gtcgagtact
     3781 ttcgcatcaa gcgatggacc gggtacttta tccaatcgtc catgcgcgca gcctagccgg
     3841 acagtccccc tgcatcccac aactggcagc gctaccctgc agtatggtat ctccctcgca
     3901 tggccaagcc acgcgtcctc cttgtcattc ccccgctgac ccaactcaat acgccctatc
     3961 ccgcgaccgc ctacctgaca ggcttcctcc gttcacgagg atatgagacc gcgcaagccg
     4021 acctcggcct cgaaatggtg ctgcgcctct tctcgcggtc gggactggag caggctttca
     4081 cccggatcaa gcagacggag cggccgtggc gaggcgaaat ccaacaaatc ctgacgctgg
     4141 aaacggccta tcttcagacc atcgagccgg taatcgcgtt tcttcagggg aaggacccga
     4201 tgttggcccc ccacctcgcc cgcccgggat tcctcccgga gggcccccgt tttgccgctc
     4261 atcgtcggcc cggagaagga tcgcaagcat cttcgccgtc agacaccgcc cggcaccgcg
     4321 ccacactata cattgaggac ctcacggacc tcctccacag caccgtcgct ccccacctct
     4381 ttctgaatcg ttacgccgaa cagattatgc aggccacctc atcattcgac cggatcgagg
     4441 acgccctggc tcaaccactg agcctcacag atgaattgct gcttgaggcg ctctggccgc
     4501 atctccaccg aaccacgccc gatgtcgtag gcctcacaat cccctttccc ggcaacttgt
     4561 tcggtgcgtt gaggattgcg caggcgatca aggcacaatc accacctacc gtcatcgtcc
     4621 tgggtggcgg atatgtgaac accgagctga gacgactcga agacccacga ctgttccagt
     4681 acgtggatta tgtgacgctg gatgacgggg aacgcccctt cctctgcctc ttggaacacc
     4741 tggcaggcgg gcggcccgag cccctgctcc gacggacgtt tctctgcaga gacggccgcg
     4801 tcatatttcg agacggcagc aatgaaccgg acatttcgat gcatgagctc ggccgaccga
     4861 cctatgccgg actcccactg accgactacc tctcaatcct cgacagcctg aaccccatgc
     4921 atcgactctg gtcggaaggc cattggaaca agttgaccgt ggcgcacggc tgttattgga
     4981 agcaatgcac gttctgcgat gtgggattga actatatctc gcgctatgag gtaactccga
     5041 ccgacgtcct gatcgaccag atcgaggcgt tgattcaaga aaccgggcaa cgaggcttcc
     5101 atttcgtcga tgaagccgca ccgccggccg cgctcaaagc cctggccctt cgcctgctcg
     5161 aaaccggcct gaccatttcc tggtggggga acattcgatt cgaggaggcg ttctcccccg
     5221 atttggcacg gctgctggcg gcatccggct gcatcgccct taccgccggg ctcgaagccg
     5281 cgtctgatcg cttgttggaa cgaatgaaga aagggattac ggtagaccaa acggcacggg
     5341 ttgcggcggc atttcgccag gccggcatcc tggtgcatgc ttatctcatg tacgggtgtc
     5401 ccggtgagac catcgccgac acggtagatt cactggaacg tgtgcggcaa ctgtttgcga
     5461 accatctcct gcagtccgcc ttctggcaca aatttacagc cacggcccac agtcccatcg
     5521 ggctcaaccc ggctgcggaa ggcctcacga tcctcggccc gacattcgaa gggtttgccg
     5581 aaaacgatct ccgccacgcc gaccccaacg cgacctgccc accctggctc ggcgaaggcc
     5641 tgcgcgctgc gttgctcaac tacatggaac aggccggatt cgatatcccg tcacaacgct
     5701 ggttctcaca tcgggtcaaa ccgccacggg taacgaagac ctggctcacg caggcgttgg
     5761 cggcttcctc cgacgtgcca ttgactgccg aacgacgttg cgtctggatc ggcggcaggc
     5821 ctgtgatcgc ggcaacagga cgtacgcgca ccagggtcat tcttccgaca cgcacagaag
     5881 attttacgat cagcctggcg tctccgcaca ccgaatggct gctccgcttc ctgcacgaat
     5941 cgactccttc gctcagccgg aggaatcacg catacccccg cctcgccgaa gtccgcgatc
     6001 gatttccggc cgccggcgcg cgcggattcg atcgattcag caggggctcc gcctggcagt
     6061 cgatgcgcca agctggcctc ctcctggtat aagaccttcg ctcctccgca catccgtgac
     6121 ttccgaatac ttacgcgcac cgacctaaat gcgagtgtat ggttcagcca acatggagtg
     6181 gatcgttctg cgacattcgt tcattcatga agcggacaaa aacatgattt gtagtactta
     6241 ttagtatttt gttgtaaaca aggaaaaaaa cgtgctcaac gtgttgacat atatgcgtac
     6301 ttacactaca tgttgggtct acttatgatc caactttacc gaggagatac actatgctca
     6361 tcacacgatt gctaacccca ttgctggcta ccggcattct cttgtcggcg ccgctttcgg
     6421 ccttagctat cgccgtctac tcgtcttccg gtccaaacgc cgcatcgatt caaggttctg
     6481 tcaatggctt cagaagcgat ctcggaacga acaacggcgt gggtggctcg ttcgcaagcg
     6541 gtcgccgaga aatcaactgg gatggggtgc ctgccggatt ctctgacccg aataatctcc
     6601 cgggaaattt cttcaattcc aattcagctc gcggcgctgt atttgaaacg ccgggatccg
     6661 cattcaaggt cagtgcaaac tctggagtgg gtacgccgga gcgattcggc agcatcaacc
     6721 catctttccc gaatagtttc tcggtcttca gttcccagaa actctttacc gccatcgaca
     6781 gtaacatcgt ggatgtaaga ttctttgtac ccggcaccaa tcaacccgga gccgtgcgcg
     6841 ggttcggctc ggtcttcacc gacgtcgatt tggccggtcc taccacgatt gagttttttg
     6901 actttcacaa caacagcctg ggcaaacaaa ctgtcctcaa cacgccgggt gtgaccagtc
     6961 tttcctttct gggtgtctct ttggagcagg ccatcattaa ccgcgtcagg attaccgcag
     7021 gaaacgccgc cctctcggcg ctggacggat tcgactttgt cgccatggat gatttcatct
     7081 acggcgaacc gcaagcagtc ccggctccgg ccgccgtcct tctcctcgga agcggcttgg
     7141 ctgccctggt gtgggcgcgg cggaagtctc tccttcctga atagccacgc tccagcaact
     7201 ttgtacgaca agggggagtc agcctgaagc tgactccccc ttgtgcattc cacacatcgc
     7261 ttcctccaga ttacggctct tactgaaacg aaaagaggag ccgacctatt ggcaggctcc
     7321 tctcatctcg accatcgtcg aagctcgcgc tacttattgg gctgcggagt cagccgaaga
     7381 tagggcttga tcgatttgaa gcccttcgga aacagcgtct tggcttcggc atcgttcacc
     7441 gccggggtga tgatgcaatc ctgcccgtcc ttccagttca ccggggtcgc caccttgtat
     7501 ttcgccgtca gctgcaggga atccaccacg cgcagcaatt cgtcgaaatt cctcccgcag
     7561 gaggccggat aggtcagcat caacttgatc ttcttatccg gcccgatcac gaacaccgaa
     7621 cgcaccgtca tgctatccag cgagttcggg tggatcatgt catacagatc ggcgaccttc
     7681 ttctccgggt cggcaatgat cgggtaactg accgtacaat tctgggtttc gttgatgtcc
     7741 ttggtccagc cgcgatgcga atccaacggg tccacgctga tggcgagaat cttgacgcca
     7801 cgcttatcga actccccttt gatcctcgcc atatacccca attccgtcgt gcagacgggg
     7861 gtataatcct tcgggtgtga aaacagaatc ccccacccgc cgcccagcca ctcatgaaag
     7921 ttgatcgtcc cttccgtcgt ctcagctgta aagt
//
LOCUS       DAORRQ010000112         7742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_378_length_7742_cov_894.063874, whole genome shotgun sequence.
ACCESSION   DAORRQ010000112 DAORRQ010000000
VERSION     DAORRQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_378_length_7742_cov_894.063874"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PK224_19660"
     CDS             complement(<1..564)
                     /locus_tag="PK224_19660"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HRB18022.1"
                     /translation="MPNARGRTVKRRIWWRGSMAVAAVLLVSGGAVSPVEAKTLPHLR
                     QVASMRPADSLKVMPLPEGKRSEPAVEAPASRSEAAVSASGDSLSTEFSGTSYDPSGR
                     RDPFLPMIQLGQQTEQDVTLPPLQRVGLTELSLIGVLWGNYGYTAMVQTPDGKGYSIR
                     RGTRIGPNNGVVSSITERGIIVQERFTD"
     gene            complement(582..1205)
                     /gene="pilO"
                     /locus_tag="PK224_19665"
     CDS             complement(582..1205)
                     /gene="pilO"
                     /locus_tag="PK224_19665"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRB18023.1"
                     /translation="MSLNAISLDGLRSIPTGQKVALLGLLVAAILVGFYFYVVDPKTM
                     ELEAVQGQVAQLDTEIQNLTLKVKHLDELVAASKQLEIELAAKKERLPPEEEAVMLLK
                     QVSDLGMRLGLDVRLWKPGTQAEDPSKLFIRMPINVEVAGGYHTAAIFFDRISKLSRI
                     VTVQDVRIGAARVDQGRVVTQTVFDLVAYAAPGEKKAAAPVPAAKPK"
     gene            complement(1207..1761)
                     /locus_tag="PK224_19670"
     CDS             complement(1207..1761)
                     /locus_tag="PK224_19670"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRB18024.1"
                     /translation="MIRINLLATGPRSRKAKPQWDVRAEALIGIGVLLVTLTGCWYYA
                     ASLDEDIQAKQSEKQVKDKQVAQLKEQVKAVQDFEEKKKQLEAKNRIIDQLEKSRTGP
                     VKVLDHVSQSLNPLKVWLVRLNLKGNNVELEGRALTNDDVVEFVNNLRRTDQFGAIKL
                     MESRAGLDNKMNTYQFKLDLSMKG"
     gene            complement(1758..2855)
                     /gene="pilM"
                     /locus_tag="PK224_19675"
     CDS             complement(1758..2855)
                     /gene="pilM"
                     /locus_tag="PK224_19675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRB18025.1"
                     /translation="MLASLKNLADMEFLSMFSPQRQLLGLDIGSSSIKLVQIKEHRGR
                     YTLQKFAVKELEPEVIVDGTVMDEGRVVSAIKELLAEQNVKLKQVAISISGHAVIVKK
                     ISLPPMPDEELDAQVKLSAEQYIPFDINEVNLDFHVLPPSENPDEQSEMSIVLVAAKK
                     DKINELTELVKAAGLSPVVMDVDAFAVENMYGVTSPATQDDTTILVNIGASVMNVNIV
                     GGGVSLFTRDIPLGGNRYSEAVQREMGVSFEEAEQLKKSDRDDDHTLASVMESVNAEV
                     ASEIARTIDYFKTTSSESEIARVLLFGGGAKVKGLAQQLRDRMHVEVEIANPFNEIDT
                     SQCDVDPDQLTEMGPLAAVGVGLALRTVGDR"
     gene            complement(2869..3084)
                     /locus_tag="PK224_19680"
     CDS             complement(2869..3084)
                     /locus_tag="PK224_19680"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRB18026.1"
                     /translation="MKKVSDVPKKKLYKTNEVCEMFDISRATLFRWEREGLITGPPRD
                     WRNWRLYTAENVEQIRHVMGGGRKEVA"
     gene            complement(3220..4101)
                     /gene="rapZ"
                     /locus_tag="PK224_19685"
     CDS             complement(3220..4101)
                     /gene="rapZ"
                     /locus_tag="PK224_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRB18027.1"
                     /translation="MLALLMAALNLVVISGLSGSGKSHALKAFEDAGYFCVDNLPPAL
                     LPTFVELCHQQGGEIKNVALGIDIRERVFFGDLAKVLGELKAQGHALQLVFLEAREEV
                     LVRRFSESRRPHPLLPHMPVVEGVRFERERLSELRKHADRVIDTSNITVHELRELLIR
                     QFRQETAARRMTISLVTFGYKFGVPYDIDLLFDVRFIRNPFFVPELKALTGEDARVQQ
                     YVLGDPAAGQFLEHLQGLFGFLVPLYERDQRSYLSIGIGCTGGRHRSVTMAVHLREQF
                     AALGYDVSVTHRDMQKP"
     gene            complement(4073..4624)
                     /gene="raiA"
                     /locus_tag="PK224_19690"
     CDS             complement(4073..4624)
                     /gene="raiA"
                     /locus_tag="PK224_19690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRB18028.1"
                     /translation="MRLMITGRHVAVTPALREYIETRMERLDRYGLKLGTLQILLSVE
                     KFHHVAEVVGVVQSRRLQAKTSTEEMYASIDEVVDKLGAQLRKLKERKVNHKFGDQPR
                     VRAVARKAPRAARNEVEVVRPTLEVLTVEEAVEALNSAKLAVLVFVNALSGTVQVLQR
                     LPNGRVSLIDPASDRARTAHGRA"
     gene            complement(4731..6248)
                     /gene="rpoN"
                     /locus_tag="PK224_19695"
     CDS             complement(4731..6248)
                     /gene="rpoN"
                     /locus_tag="PK224_19695"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRB18029.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLQQHLMEN
                     PLLDELATETEESEETATAEREQPDTSTTVSSEVRGEGDDKPAESGENAEEFSASSWE
                     DYFDTDMRRGETEYSSSSKEEFPSYEQTVAKSTSLEDHLVWQLSLSGLSDREKAIGRL
                     IIGNLDDDGYLRISLDELVAGTDYSVAEAESVLKDVQGFDPNGVAARDLSECLLLQLK
                     FLGRSQIGSLGSRPGVLKGTVLESIVLHHLKDLEKKQYNRIAKALNISMDDVFEATRI
                     IEGLEPKPGRPFSNTQNYAIVPDVFVVKNEGVWEVLLNDDGLPRMRISPYYKQLMSSG
                     QSGSAETKAYLDDKLRAAQWVIRSIEQRNKTIVKVVSSIVKFQEGFFERGIQHLKPLV
                     LKQVAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADQPTDMLSSLTVRE
                     MIRQMVAAEDARKPLKDEEIAARLRTQQVLIARRTVAKYRAEDNIPSATQRRKFF"
     gene            complement(6269..6994)
                     /gene="lptB"
                     /locus_tag="PK224_19700"
     CDS             complement(6269..6994)
                     /gene="lptB"
                     /locus_tag="PK224_19700"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRB18030.1"
                     /translation="MPDRLAANGLVKSFRGRKVVKGVSLDVQAGEVVGLLGPNGAGKT
                     TIFDMMVGLCQPDEGTIALSGQEITDLPMYKRARRGIGYLPQESSVFRRLSVEHNILA
                     ILEMLGYSRSERMERVETLLKELDLVHIRTSKAYALSGGERRRLEITRALATSPSFML
                     LDEPFAGIDPIAVADIQQIIIRLKQRNIGVLITDHNVRETLSITDRAYIINEGTILEA
                     GPPGVIEKSEMARAVYLGEGFRL"
     gene            complement(7051..7644)
                     /locus_tag="PK224_19705"
     CDS             complement(7051..7644)
                     /locus_tag="PK224_19705"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRB18031.1"
                     /translation="MWCLLLSGVALQTLPWAHGAEPSVAKESTSNPAPTTITSRTMTV
                     SNQANTAIFDGAVVLTRGLLVVHSDHMVVSFHPNRNGADSKNANGTTKPKNNSSANKP
                     EPQGQDAAPAVSERSIRMVEATGRVRIEKEDGRATCQKAVYYEDEKKIILTGDPVAWQ
                     KGTRVSGKRIIMFLDEDRSVVEGDSQVVIEGESGGKR"
BASE COUNT         1587 a         2471 c         1940 g         1744 t
ORIGIN      
        1 gtccgtgaac cgctcttgaa cgatgatgcc tcgttcggtg attgaactga ccacaccatt
       61 gttgggcccg attcgtgtcc cgcggcggat gctgtaccct ttgccgtccg gcgtctgaac
      121 catggccgtg taaccgtaat tgccccagag caccccgatc aaactcagtt ccgtgaggcc
      181 gacgcgctgc agcgggggaa gagtcacatc ctgctctgtc tgctggccca gctggatcat
      241 aggcaggaag ggatcccgac gacccgacgg atcgtacgag gttcctgaaa attccgtcga
      301 caatgaatcc cccgatgctg agaccgccgc ttcggaacgc gaggccggag cttcaacagc
      361 cggctcagac cgcttcccct cgggcaacgg catgaccttc aaggaatcgg ccggccgcat
      421 ggacgccact tggcgcaagt gaggcagtgt tttcgcctca acaggcgaca ccgcgcctcc
      481 actcaccagc aggaccgctg caacggccat cgacccgcgc caccagattc gtctcttcac
      541 ggtgcgtccc ctcgcgttgg gcataagcta ggctccaatc ctcactttgg cttcgccgca
      601 ggaacgggcg cggcggcctt tttctctccc ggggcagcat aggccaccag atcgaacacc
      661 gtctgcgtga ccacacgtcc ttgatcgaca cgagccgcgc cgatccgcac atcctgaacc
      721 gtcacgatac gggacagctt gctgatacga tcgaagaaga tggcggctgt atggtatccg
      781 ccggccactt ccacgttgat tggcatccga atgaagagct tcgacggatc ctcagcttgc
      841 gtccccggct tccagagacg gacatccaac cccagtcgca tgccaagatc ggacacttgc
      901 ttgagcagca tcaccgcctc ttcttccggc gggaggcgtt ccttcttcgc ggccaattcg
      961 atttccagct gcttgctcgc cgccaccaat tcatccagat gtttgacctt caaggtcaga
     1021 ttctgaatct cggtatccaa ctgggcgacc tgaccctgaa ccgcttccag ctccatggtc
     1081 ttgggatcga ccacgtagaa atagaaccct accaaaatcg ccgccaccaa cagtccgagc
     1141 agcgcgactt tctgacctgt cggaatgctg cggaggccgt ctaagctgat tgcattcaga
     1201 ctcatgttag cctttcatcg acaggtcgag tttgaattga taggtgttca tcttgttgtc
     1261 cagtcccgcc cggctctcca tcaacttgat ggcgccgaat tgatcggtcc gccgcaggtt
     1321 gttcacgaat tccaccacat catcgttcgt cagcgcgcga ccttcgagct cgacgttgtt
     1381 gcccttgaga ttcagccgca cgagccagac cttcagggga ttcagactct gactgacgtg
     1441 gtccaacacc ttgaccggtc ccgtccggct cttttcgagc tggtcgatga tgcgattctt
     1501 cgcttcaagc tgcttctttt tctcttcaaa gtcctgcacg gccttgactt gctctttcag
     1561 ctgagcaacc tgcttgtcct tgacctgctt ctcgctctgc ttggcctgga tgtcctcatc
     1621 aagggaagcg gcgtaatacc aacaccccgt caaggtgacc agcaacaccc cgatgccgat
     1681 caaggcctca gcacgtacat cccactgcgg tttggctttc ctggaccggg gtccagttgc
     1741 gagcaagttg attctaatca tcggtccccc accgtccgga gcgcgaggcc tacccccacc
     1801 gccgcaagcg gccccatttc ggtcaactga tcgggatcga cgtcgcactg cgaagtgtcg
     1861 atctcattaa agggattcgc gatttccacc tcgacgtgca tgcgatcgcg aagctgttga
     1921 gccaggccct tgaccttagc tcccccgccg aacagcaaga cccgcgcaat ttcgctctcc
     1981 gaagaggtcg tcttgaagta atcgatcgtg cgggcgatct ccgacgccac ttccgcattc
     2041 acactctcca tgaccgatgc tagtgtatga tcgtcgtctc ggtcgctttt cttgagttgc
     2101 tccgcctctt cgaacgagac gcccatctcc cgctgcacgg cctcggaata ccggttccct
     2161 cccaagggaa tatcgcgagt aaagagggag actccccctc ccacgatatt cacgttcatc
     2221 acgctggccc cgatgttcac cagaatcgtg gtgtcgtctt gcgtggcggg cgaggtcacg
     2281 ccatacatgt tctccaccgc aaatgcgtcg acatccatca cgaccggact cagtccggcc
     2341 gccttcacca gttcggtcaa ttcgttgatc ttgtcctttt tcgccgcgac cagcacaatc
     2401 gacatttcgc tctgctcgtc cggattctcc gacgggggta agacatgaaa gtccaggttc
     2461 acttcattga tgtcgaaggg aatgtactgt tcggcagaca gtttcacctg tgcatcgagc
     2521 tcctcatcgg gcatcggcgg cagggaaatt ttcttcacaa tgaccgcatg gccggagatg
     2581 gagatggcga cctgcttcaa tttgacattc tgttcggcca acaattcctt gatggccgac
     2641 acgacacgcc cttcgtccat gaccgtgccg tccacgatca cttcaggctc caattccttg
     2701 acggcaaact tctgcaacgt ataccggccc cgatgttcct tgatctgcac cagtttgatg
     2761 ctgctcgaac cgatgtcgag ccccagcaac tgccgctgcg gagaaaacat cgagagaaat
     2821 tccatatccg ccagattttt cagtgaagcc agcatgctca tgccctctct acgcgacctc
     2881 ttttcgtcca ccacccatca cgtgcctgat ttgttcgaca ttttcggcgg tatacagacg
     2941 ccaatttcgc cagtcgcggg gtggtccggt gatcaatcct tcacgctccc agcgaaataa
     3001 cgtcgcgcgc gagatgtcga acatctcgca gacctcgttg gtcttgtaca atttcttttt
     3061 ggggacatcg ctgactttct tcacgaaccg cctcaaacgc cgctattcat cgcatgtttc
     3121 gagcccacgg aattaagtat agttgatata gtccacctgt caagaaagtg ggctctccag
     3181 gggcttctcg ggaagaaacg gcgaaacagc gctcaggaac tagggttttt gcatgtcgcg
     3241 gtgggtaacg gacacatcat aaccgagggc ggcaaattgt tcccggaggt gcaccgccat
     3301 ggtcacagac cgatgacgcc ctccggtaca accgatgccg atgctgaggt agctgcgctg
     3361 atcccgctca tagagcggga ccagaaagcc gaacaagcct tgcagatgtt cgagaaattg
     3421 accggccgcg ggatctccca atacatactg ctgtacgcgg gcatcctccc cggtcaatgc
     3481 tttgagctca ggcacgaaga acggattgcg aatgaaacgc acgtcgaaga gcaggtcgat
     3541 gtcatagggc acgccgaact tgtacccgaa tgtgaccagt gagatggtca tgcggcgagc
     3601 ggccgtttcc tggcggaact gccggatcag caactcgcgc aattcatgca ccgtaatgtt
     3661 ggaggtgtcg ataacccggt cggcatgctt ccgcaattcg ctgaggcgct cacgctcgaa
     3721 ccgcacgcct tccacgactg gcatgtgcgg caacaaggga tgcggacggc gactctcgga
     3781 aaaccggcga accagcactt cctctcgggc ttccagaaac acgagttgca gcgcatgtcc
     3841 ctgcgctttt agctcgccca ggaccttcgc caaatccccg aaaaagactc gctcgcgaat
     3901 atcgatgccc agggcgacat tcttaatctc cccgccctgc tgatggcata actcgacaaa
     3961 ggtgggaaga agggccggcg gaagattgtc gacgcagaaa taccccgcgt cttcgaaggc
     4021 tttcaaggca tggctctttc cggatccgga aaggccgctg atcacgacga gattaagcgc
     4081 ggccatgagc agtgcgagca cggtcgctcg cgggatcgat cagagacact cgtccattcg
     4141 gcaagcgctg gagaacctga accgtgccgg acagcgcatt cacaaagacc agcaccgcga
     4201 gcttcgcaga attgagcgcc tcgacagcct cttccaccgt caacacttcg agcgtcggtc
     4261 gaaccacttc gacctcattg cgcgcagccc gtggagcctt ccgtgcgacc gcccgcacac
     4321 gtggttgatc gccgaatttg tggttgacct tgcgctcttt caatttccgc aactgggcgc
     4381 cgagtttatc gacgacctca tcgatcgagg catacatttc ctcggtcgac gtcttcgctt
     4441 gcaaacgccg gctctgcacg acacccacga cttcagccac atgatgaaac ttttccacgc
     4501 tgagcagaat ctgcaaggtc ccgagcttca gtccgtagcg gtccaaccgt tccatgcgtg
     4561 tttcgatata ctcccgtaga gcaggagtga ctgccacatg ccgacccgtg atcatcaatc
     4621 gcattccatc ctcgtttctc aggctgcccg caccaccgta cgtgcaagcc cgttgcgacg
     4681 attgttgtac ccccagggga acttcctccc tggtgccaga cccgaccagc ttagaaaaac
     4741 ttcctgcgtt gggtggcgga gggaatgttg tcctccgcac gatacttcgc gacggtgcga
     4801 cgggcgatca gcacttgctg cgtccgcagc cgcgcagcga tctcctcatc tttgagaggc
     4861 ttgcgcgcat cttccgccgc gaccatttgc cggatcattt cgcgcaccgt gagggacgac
     4921 agcatgtccg tcggctgatc cgcgcgctga agcccggcgt taaagaaaaa tttcaactcc
     4981 aacatcccct gcggacaata catatattta ttggccgtca cgcggctgat cgtcgactcg
     5041 tgcatcccga tatcctcggc gacttgcttg agaaccaacg gtttcaagtg ctggatcccg
     5101 cgctcgaaaa aaccttcttg aaacttcacg atgctcgaca cgaccttcac aatggtcttg
     5161 ttgcgctgtt cgatactccg aatcacccac tgcgccgccc gcaatttgtc gtccagatag
     5221 gccttggtct ccgccgaccc cgattgccct gacgacatca attgcttgta gtacgggctg
     5281 atccgcatac gcggcaggcc gtcgtcattg agcaacactt cccacacccc ttcgttcttg
     5341 acgacaaaga catccgggac gatcgcgtaa ttctgtgtgt tggagaaggg acgtccaggc
     5401 ttcggctcca acccttcgat aatccgggtc gcttcgaaaa cgtcgtccat cgaaatattc
     5461 agcgccttcg cgatgcggtt gtactgtttc ttctccagat ccttcagatg gtggagcacg
     5521 atggattcca gcaccgtccc cttcagcact cccgggcgcg accctaagga gccaatctgg
     5581 ctccgtccca aaaacttcag ttgaagcagc agacattcgg ataagtcccg tgccgccaca
     5641 ccatttggat cgaatccctg aacatctttc agcaccgact cagcttccgc caccgaatag
     5701 tccgtgccag cgaccagttc atccaagctt atccggagat acccgtcgtc atccagattt
     5761 ccgatgatca ggcgtccgat cgctttttct cgatccgaga ggcccgataa cgacaactgc
     5821 cagacaaggt gatcttctaa cgacgtcgac ttcgccacgg tttgttcata cgaggggaac
     5881 tcctccttcg aggaagagct gtactcggtt tcaccgcgac gcatgtccgt atcgaagtaa
     5941 tcttcccagc tggaggccga aaattcttcg gcgttttccc cgctttcggc aggcttatcg
     6001 tcgccctctc ctcgcacttc actggatacg gtggtcgacg tatcgggctg ttctcgctcc
     6061 gccgtcgccg tctcctcact ttcttccgtc tccgtcgcca attcatccaa caacggattt
     6121 tccatgaggt gctgctgcag actttgctga agctccaaac gagacagttg cagcaatttg
     6181 atcgcctgct gcaattgcgg cgtcataatc agtttttgac tgagcctgag atccagtcgc
     6241 agcttcatcg cacactcact ccctgccgct acaggcggaa tccttcgccc aaatacacgg
     6301 ccctggccat ttcacttttc tcgattaccc cgggcgggcc cgcttccagg atcgtgcctt
     6361 cattgatgat gtaggctcga tcggtaatcg acaacgtctc ccggacattg tggtccgtaa
     6421 tcaacacgcc gatattccgt tgcttcaacc ggataatgat ttgctggata tcggccaccg
     6481 caatgggatc gataccggca aaaggctcat ccagcagcat gaatgagggg ctggtcgcta
     6541 atgctcgcgt gatctccaga cgtcgacgtt ctccacccga caaggcatag gccttacttg
     6601 tgcgaatatg caccagatcc agttccttga gcaatgtctc aacccgctcc atgcgctcac
     6661 ttctcgaata gcccagcatc tccagaatcg caaggatatt gtgttccact gacagccggc
     6721 gaaacaccga cgactcctgc ggcaaatacc cgatgcccct ccgggcccgt ttatacatgg
     6781 gcaaatcggt tatttcttgg ccgcttaagg caatcgtgcc ttcatccggc tgacacagtc
     6841 ccaccatcat atcgaagatg gtcgtttttc ctgctccatt gggcccgaga agaccgacga
     6901 cttcgccggc ctgcacatcg agcgacacac cctttaccac ttttcgtccg cgaaaactct
     6961 tcaccaggcc gttggcagcc aggcgatccg gcatgcccat tgatgtcgct ccctgggcga
     7021 ccgatgtctg ggcgtcggaa gacatcggga tcaccgcttg ccaccgcttt cgccctcgat
     7081 gaccacctga gagtccccct ccaccacact gcgatcctcg tccaggaaca taatgatccg
     7141 cttgccggat acgcgcgtcc ccttctgcca agccaccgga tcacctgtca gaatgatttt
     7201 cttttcatct tcgtagtaca cggccttctg gcaggtcgcg cgcccatctt ctttctcaat
     7261 tctcactcgg cccgtcgctt cgaccatccg gatactgcgc tccgagaccg cgggtgccgc
     7321 atcctgccct tgtggttccg gcttgttcgc actcgaattg ttcttcggct tggttgtacc
     7381 gttagcattc ttgctgtcgg ccccattgcg gttgggatga aacgagacca ccatatgatc
     7441 cgaatgcacc accagcaggc cacgagtcaa gacaaccgcc ccgtcaaaaa tcgcggtatt
     7501 tgcctgatta ctgaccgtca tggtgcgaga cgtaatcgtc gtcggtgcag gattggaagt
     7561 cgactctttc gcaacgcttg gctctgcccc atgcgcccag ggaagggtct gcagcgcgac
     7621 gccgctaaga agcaggcacc acatcgacgc gcacgtcgtc caacacctga aactcttctg
     7681 tgtccatttt tcccagcaat cccctgccgg tgacttgcaa cccatgtccg ttaatcgtga
     7741 ca
//
LOCUS       DAORRQ010000113         7675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_380_length_7675_cov_405.320866, whole genome shotgun sequence.
ACCESSION   DAORRQ010000113 DAORRQ010000000
VERSION     DAORRQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_7675_cov_405.320866"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..432)
                     /locus_tag="PK224_19710"
     CDS             complement(70..432)
                     /locus_tag="PK224_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18032.1"
                     /translation="MTRSSHRRFVVHCILSAAAMGCAEKGRIIDSGLVGAPHPTSHSL
                     IDGDYLALKLQVPEDRRPVSVGLARVTWAQGNRFGVELLMMDADERARLNGFLEENLP
                     LELEFQDSHQELTITAAE"
     gene            complement(770..1966)
                     /locus_tag="PK224_19715"
     CDS             complement(770..1966)
                     /locus_tag="PK224_19715"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HRB18033.1"
                     /translation="MSKRLILCFDGMWDLPPDPSRQLPSSSTPNTQTLHNSHRHLSPA
                     TNVLRLYHSILPRTEDGRAQQKWYDSGADIPWFYRFRPGSFGYGVDQTILHAYAYLAA
                     TYEPGDELFVYGFSRGAYTARSLVGLLTTAGLIPAGLLHTDLVKRVREVVSHPSPSTT
                     TPTALRQSLHEMILEPSNQALDDAYRLYRKGHGDILAGMPTSGRRRGTLEVPITLLGL
                     WETVGPLGIPTNALKWLNDHRYNFHDTELSSIVKQAYHALAIDEHRADHNATLWTSPA
                     CPGQTIEQRWFAGAHGDLGGAYPERDLADVALAWLQRHSMEKGLAIETRLVPLQPNAL
                     SPIHDSFSECFGRIRKWFHSRFYRPVMQTGTNTEVLDSSVHTRLIHTPHYRPKNEGLK
                     SVLHFE"
     gene            complement(2358..2921)
                     /locus_tag="PK224_19720"
     CDS             complement(2358..2921)
                     /locus_tag="PK224_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18034.1"
                     /translation="MRRPTRGLKYRLTIMLLGTYLVGAGCVHHIHVTSPSSDRAAAPI
                     PATVQLEVPFLAIEGADHMPGIPLLEWPSKDLQQTIVEYFTQRQTFTSIGTEAGDMRL
                     VVKAWLMLRAPDRYLYRVHLESDLAFSGQAPLKTYAAEGEALGSSVRWVTASDQEPIA
                     EATAQALHQLATQIEQDREFVIKATHR"
     gene            complement(2936..3421)
                     /locus_tag="PK224_19725"
     CDS             complement(2936..3421)
                     /locus_tag="PK224_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18035.1"
                     /translation="MAIRSRTASMWHGLVLLTACLTLAHCSHDIHEKRADTVKDHVEA
                     FYDHLTHDRVAAAVRENEAIEHLSSQLGDIISRRVNRPGTNQVDREWTDLRTANETAA
                     QNWLALGQYLSIKKQYAQSRATYQRVIDTYTGTTERTYREQAARAIRDLDILTPPPSS
                     H"
     gene            complement(3524..5494)
                     /gene="glgB"
                     /locus_tag="PK224_19730"
     CDS             complement(3524..5494)
                     /gene="glgB"
                     /locus_tag="PK224_19730"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRB18036.1"
                     /translation="MDPLANHRAGEHLDPATPATRLTDERRSLLTANDLYLFNEGSHF
                     HLYDKLGAHPATLQGVEGTYFAVWAPEAEQVSVFGTFNHWDPTRHPLHPCQFSGIWEG
                     FVPGVGVGALYKFHIRSRHHGAVLIKTDPFARLNEIPPKSASVVWNLDYTWQDGDWMH
                     TRKRHNALDAPISIYEVHLGSWMRVPGEGHRSLSYREAAPKLIEYVQRLGFTHVEFLP
                     LMDHPFFGSWGYQTTGYFAASGNYGTPQDLMYLIDQLHQHDIGVILDWVPSHFPTDEH
                     GLSRFDGSHLFEHADPRQGLHPDWGTAVFNYSRNEVRSFLISSALFWLEQYHADGLRV
                     DAVASMLYLDYSRKEGEWIPNQHGGRENLEAITFLRQLNEEIYRRHPDVQTFAEESTS
                     WPSVSRPTYAGGLGFGMKWDMGWMHDTLEYMALDPVYRKHHHRNLTFRMLYAFQENFL
                     LPLSHDEVVHGKGSLLGKMPGDDWQKFANLRALFGYMYAQAAKKLIFMGGEIGQWREW
                     AHDDSIDWNLLQYQPHQGLQRWVADLNHLYRTEPALHEFDFDPRGFEWIDCQDVDAGV
                     ISLLRHGRTPQQHIAVVCNFTPVPRLQYRVGVPQGGYWKELLNSDASMYGGTGLGNLG
                     GLSAEPIPAHDRTHSLAMTLPPLSVLFFKAPS"
     gene            5657..5734
                     /locus_tag="PK224_19735"
     tRNA            5657..5734
                     /locus_tag="PK224_19735"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5693..5695,aa:Glu,seq:ttc)
     gene            5980..>7675
                     /locus_tag="PK224_19740"
     CDS             5980..>7675
                     /locus_tag="PK224_19740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRB18037.1"
                     /translation="MSVAVRWFHAVRSPLYSGMTERQWYACCAVILVVTAAIDAYTPL
                     GIAAGVGYLLAVVVAGMVCEVRFPWRVAAVSIGLTVLGFFWSPPGGVVWIGATNRVGL
                     AMAILLTAWIVWQWNMAVRGRSHDLAVRLQSILTHSQTSIFLKDLQGRYLEVSEQFLS
                     LTGFSAEMVIGKADRDIFPPELVAIYREHDAQVIKAGAAMNFEETVPLGDGLHLNIVC
                     RFPLRDGQGSIVAIGGIATDITARLQAEAARSEAQERFDLVVAATQTGIWDWDLHTNR
                     MYYCPLWKASLGYQEHEISNSPTEWESRLHPDDKARACALVDDYLSGRIGAYELEHRL
                     RHKDGSYRWIQTCALLQRDADGRPRRMTGSHVDITERKRAEDALTQSESTLRSFFNSG
                     AMMMGIVELLDGDILHVSDNRCVASFFGTTPELMQGRLASELGAPPDILALWHRSYEE
                     SERTKQPVRFEYVHPQKGSGVELRLSATVCWIGIGPSGRSRYAYVVDDVTEARCLAEA
                     LGQTAERYHGVVTALAEGVVLHDEQGRIIACNPSAERILGLTADQLMGRTPVDPGWQ"
BASE COUNT         1564 a         1988 c         2427 g         1696 t
ORIGIN      
        1 gagatggaag ccactcggca tcatgcccac ctttttcaag agaactattc ctgccgtctg
       61 tacgtggggc tattccgcgg ctgtaatcgt gagttcctgg tgtgagtctt gaaattccag
      121 ttcgaggggg aggttttctt cgaggaaacc attcaatcga gcccgttcat cagcatccat
      181 catgaggagt tccactccga atcggtttcc ttgtgcccag gtgactctcg ccaaacctac
      241 cgaaaccggt cggcgatctt caggaacctg cagtttgagc gcaaggtagt ctccgtcgat
      301 gaggctgtgt gaggttgggt gcggcgcccc gacgagaccg gaatcgatga tccgcccttt
      361 ttctgcgcag cccatggccg cagcagaaag gatgcagtga acgacgaatc ttcgatgcga
      421 tgaacgtgtc atgcgaacct cccgcgtgtg gcgaacgagc acgacgaaac cccggtgatg
      481 aacctatctc ggctatgacg gatcagatag tctgcgaaga gggtacttct aacgaggggc
      541 acgtcagggg cgcggagcct taaggatctg tggcgagcgt cctagggttg aggaaaatag
      601 gccattgaag agtacgaatt aacgggcagt aggtgtgtct gtggaggtga gcatcgaagt
      661 gcttggtggg ggttgttgtt cggtcaggta gcgttctaag gctgtgtcgt cctgcttttc
      721 aaggcgtcgt gtcgcgggag gttggtagat ttggtgaata caacagggct cattcaaaat
      781 gtaagacgct tttcaagccc tcgtttttcg gtcgataatg gggcgtgtgt atgaggcgtg
      841 tatggacgga gctgtccagc acttcggtat tcgttcccgt ctgcatgact ggtcgataga
      901 atcgtgaatg aaaccacttc cggatccttc caaaacactc gctaaacgaa tcgtgaatcg
      961 gacttagcgc atttggctgc agcggaacca aacgggtttc aatagccaat cccttctcca
     1021 ttgagtgccg ctgtagccag gccaaggcga catctgccaa gtcccgttcc gggtaggccc
     1081 cgccgagatc accgtgtgcc cctgcgaacc agcgttgttc aatggtctgc cccgggcagg
     1141 ccggtgaggt ccacagcgtc gcgttgtgat ctgcgcggtg ttcgtcgatg gcgagtgcat
     1201 gataggcctg ttttacgatc gaactcagtt cggtatcgtg aaaattatag cggtggtcgt
     1261 tgagccattt gagggcgttg gtcggaattc caagtgggcc gacggtttcc cacaacccga
     1321 gtagcgtgat cggaacttcc agtgttcctc gccgtctccc ggaggtaggc attcctgcaa
     1381 ggatatcgcc gtgaccctta cgatagaggc gataggcatc gtcgagggcc tggttggagg
     1441 gctcaagaat catctcatgt aggctttgtc gaagagcagt gggtgttgta gtcgaaggtg
     1501 acggatggga gacgacctca cgcacgcgct tgacaagatc ggtatgtaga agcccggcgg
     1561 gaatgaggcc ggcggtggtg agcaggccga cgagagaacg tgcggtatac gcacctcggc
     1621 tgaagccata gacaaatagc tcatcaccgg gctcgtaggt ggcggcgagg taggcgtaag
     1681 cgtgaaggat ggtctgatcc actccatacc caaatgagcc aggccggaac cgataaaacc
     1741 agggaatgtc ggcaccggag tcataccatt tctgttgcgc acgtccgtcc tccgtccgcg
     1801 ggaggattga atggtagagc cgcagtacat tcgtggccgg agatagatga cggtgggagt
     1861 tatgtagggt ttgtgtgttg ggagtgctcg atgagggcaa ctgcctgcta gggtctggag
     1921 gtaaatccca catgccatcg aagcacagaa tcaaccgttt ggacacgcga cttctttctc
     1981 ctaggcgcac gagaaacggt tagagctgtt cttgtgatcc gcttgcttcg ataggaagag
     2041 agcaggccca agcagcgctg tagccgcatt gctcgcccat ggtagagcaa acttaatgcc
     2101 attattgttt ggtgccgggg tgagttggaa ttgtgtcgat gcaggtgatc gatagccatg
     2161 tgaacaactc aatcactgcc ttttcaggct gaatcctgag gaaagagagg aaataagggg
     2221 ctatctcacg atagacctgt atcaaatggg atacggattc gtatccgaaa gcatacgtgc
     2281 atatgtgaca tgtgtgcgtg agtcgcgagt cgaggtggag tcgaggctgt ggctcgaagc
     2341 agatatgtca ggagagttta tcggtgagta gccttgatga caaactcgcg atcttgttca
     2401 atctgagtgg ccaattggtg cagggcttgg gcggtggcct cggcgatcgg ctcttgatcc
     2461 gatgcggtaa cccatctcac tgaggagcca agggcttcac cttcggctgc gtatgtcttg
     2521 aggggggctt gtccggaaaa ggcaaggtcg gattccaaat ggacccggta gaggtagcgg
     2581 tcaggcgcgc gaagcatgag ccaggctttc acaaccaggc gcatgtcacc ggcttctgtt
     2641 ccgatagacg tgaaggtctg gcgctgagtg aaatattcga caatcgtttg ttgtaagtct
     2701 ttcgacggcc attcgaggag gggaatacct ggcatgtgat ccgccccttc tatggcgaga
     2761 aacggtacct caagctgcac cgtcgcgggg atcggggccg cagccctgtc gctcgatggt
     2821 gaggtgacgt gaatatgatg cacacacccg gccccgacca gataggtgcc aagcaacatg
     2881 attgtgagtc ggtacttgag accccgtgtg gggcgacgca ttccgaactc catggttagt
     2941 gggaggaagg gggcggggtg aggatgtcta agtcacgaat ggctcgcgcc gcctgctccc
     3001 gataggtccg ctctgtcgtg ccggtgtagg tatcaatgac acgttggtag gttgctcggg
     3061 attgcgcata ttgcttcttg atcgagagat attgtcccaa ggccagccag ttctgggcgg
     3121 ccgtctcatt ggcggtgcga aggtcggtcc attcgcgatc gacctggttg gttcctggac
     3181 gattgacgcg ccgagagatg atgtcgccta actgggatga gaggtgttcg atcgcttcat
     3241 tctcccggac ggccgctgcc acgcgatcat gtgtcaagtg atcgtagaag gcttcgacat
     3301 gatccttaac ggtgtcggcc cgtttctcgt ggatgtcgtg ggagcagtgc gcgagggtga
     3361 ggcaggccgt gagcaatacc agcccgtgcc acatcgaagc tgtacgtgag cgaatcgcca
     3421 tcgtcgcctc ctcggtaaat cggacgtttg aggtccgatg tttttcgtct cgtgtcgcga
     3481 tcctaccgta gctgggtgtg gaaaggcgag cctagggacg gcgctaggag ggggccttga
     3541 agaacagcac agagagcggg ggcagcgtca tggccaacga gtgcgttcga tcgtgggccg
     3601 gaatgggctc agccgagagg ccgccgagat ttccaaggcc ggtgccgcca tacatggacg
     3661 cgtcgctgtt caacagttct ttccaatagc cgccttgtgg gacaccgacg cgatactgca
     3721 gccgtgggac tggcgtaaaa ttacagacga cggcgatgtg ttgctgtggt gttcggccgt
     3781 gtcgcaagag actgatgacc ccggcatcca cgtcctggca gtcgatccac tcgaaccctc
     3841 gcgggtcgaa atcgaattca tgcagcgccg gctctgtccg gtagaggtga ttcaggtcgg
     3901 cgacccaccg ttgcagccct tgatggggct ggtactggag caggttccaa tcgatgctgt
     3961 cgtcgtgggc ccattcgcgc cactggccga tttcaccacc catgaaaatc agtttttttg
     4021 cagcctgggc gtacatatag ccgaacagcg cacggagatt ggcgaatttc tgccaatcgt
     4081 cgccgggcat tttgcccagg agcgagcctt tgccatggac gacttcgtca tgggacaggg
     4141 gaagtaaaaa attctcttgg aacgcgtaga gcatgcggaa ggtgaggttc cggtgatgat
     4201 gttttcggta cacggggtcc agcgccatgt actccagcgt gtcatgcatc catcccatgt
     4261 cccatttcat gccgaagccc aatccgcccg catacgtcgg acgtgacacg gacggccagg
     4321 aggtggattc ttccgcaaag gtctggacgt ccggatggcg gcgatagatc tcttcgttga
     4381 gttgccgtaa gaaggtaatg gcctccaggt tttcccggcc gccatgctgg ttgggaatcc
     4441 attccccttc ctttcgcgaa tagtccaggt acagcatcga cgcgacggca tctacccgca
     4501 gcccgtcggc gtggtattgc tccagccaga agagggcgct gctgataaga aaactgcgga
     4561 cctcgttgcg actgtagttg aacaccgccg tcccccaatc gggatggagg ccctggcggg
     4621 ggtccgcatg ttcaaagagg tggctgccgt cgaatcgact gagtccatgt tcatcggtcg
     4681 gaaaatggga cggtacccaa tccagaatca ctccgatatc gtgctggtgg agctggtcga
     4741 tcaggtacat aaggtcctgc ggcgtcccat agttgccgga ggccgcaaag tacccggtcg
     4801 tttgatagcc ccaggagcca aagaagggat ggtccatcag gggcagaaac tccacatgag
     4861 tgaagcccaa tcgctgtaca tactcaatca gtttcggggc agcttcgcga tagctgaggg
     4921 agcggtggcc ttcgccggga acccgcatcc acgagccgag atggacctcg taaatgctga
     4981 tcggggcatc cagcgcattg tgccttttac gtgtgtgcat ccagtcgccg tcctgccagg
     5041 tgtaatcaag gttccagacc acggatgcgg atttcggcgg aatttcgttc agtcgtgcga
     5101 acgggtctgt tttgatgagt accgcgccat ggtgccgtga ccggatgtga aatttgtaca
     5161 gcgcgccgac tcccacaccg gggacgaacc cttcccaaat gcctgaaaat tgacaggggt
     5221 ggagtgggtg gcgagtgggg tcccaatggt tgaacgtccc gaagaccgag acctgctcgg
     5281 cttccggcgc ccacaccgca aagtacgtgc cctcgacacc ctgcaaggtc gcgggatgcg
     5341 cgccgagttt atcgtacagg tggaaatgag aaccttcgtt aaagaggtag aggtcgttcg
     5401 ccgtgagcag actccggcgc tcgtccgtga gtctcgttgc cggcgtagcc ggatcgagat
     5461 gttcgccggc gcgatgattg gcaagggggt ccacaggtgc agagtactgt ggttgagggc
     5521 agatcgtcaa tcggccggcg gctcggtcgg ccggcaatgg ttccgatcgt gggactcttc
     5581 gggatcttgc tccagactag tgacacctct cgatcaagta tggtatgaat caacccgctc
     5641 gtacggaatt ctcttcgtcc ccatcgtcta gcctggccta ggacattgcc ctttcaaggc
     5701 agtaacacgg gttcaaatcc cgttggggac accacacttc tcccggttat tcgttggctc
     5761 aaccctgtcg ggaacattct ttccagacag tacagccatc tcacatataa agcttcttgc
     5821 ggcttcatgg aggatggttc ttgtcgtggg agaggatcgc tgtagggccg gcaggtagga
     5881 tcgtcgctgc cgtcgggatc tcgaccacga agctgcagct gttgcccgcc tcatgatccg
     5941 gtgtcgttac gcctgagtag cgtacggagt cgttctgtga tgagtgtagc cgtgcgctgg
     6001 tttcacgccg tccggtcacc gctgtattca ggcatgaccg aaaggcaatg gtatgcctgc
     6061 tgtgctgtca tcctcgtggt cacggcggcc attgatgcgt acacgccatt ggggattgcg
     6121 gccggggtgg ggtatctgct cgccgtggtc gtggccggaa tggtatgcga ggtacgattt
     6181 ccttggcgcg tggcggctgt ttcgatcgga ctcaccgtcc tgggattctt ttggtctcca
     6241 ccgggtggtg tcgtatggat cggggcgacg aatcgcgttg gcctcgccat ggccatcctc
     6301 ctgaccgcct ggatcgtctg gcaatggaac atggcggttc gcggtcgctc ccacgatctc
     6361 gctgttcggt tgcagtccat cctcacacat agccagacaa gtatcttcct caaagacctc
     6421 caggggcgat acctggaggt gagtgaacag tttctgtcgc tgaccggctt ctcggccgag
     6481 atggtgatcg ggaaagctga tcgggacatt ttcccccctg aattggtcgc tatctatcgg
     6541 gaacatgatg cccaggtcat caaggccggt gcggccatga attttgaaga gaccgtgccg
     6601 ctgggcgatg gcctgcactt aaatatcgtt tgcaggtttc cgttgcgtga cgggcaggga
     6661 tcgattgtgg ccatcggcgg gattgccacg gatatcaccg ccagattgca ggcagaggcc
     6721 gctcgaagcg aggctcagga acgtttcgat ctcgttgtgg ccgcgacaca gactggaatt
     6781 tgggactggg acctgcacac caaccgcatg tattactgcc cgctgtggaa ggcgagcctg
     6841 ggatatcagg agcacgagat ttccaactca ccgacggagt gggagtcccg attgcatccc
     6901 gatgacaaag ctcgtgcatg tgcgcttgtg gacgactatt tgtccgggcg catcggcgca
     6961 tacgaactcg aacaccgcct gcgtcacaaa gacggcagct accgttggat tcagacctgt
     7021 gctctgttgc aacgagatgc cgacggacgg ccccgccgga tgaccggctc gcacgtggat
     7081 attaccgaac ggaagcgagc cgaagacgcg ttgacccaga gtgagtccac attgagaagt
     7141 ttcttcaata gcggcgcgat gatgatgggg attgtggagc tgctcgacgg cgatattctg
     7201 catgtctcag acaaccgctg tgtggcgagc ttcttcggca caactccgga attgatgcag
     7261 gggcgactcg cgtcagagct tggcgcccca ccggacatcc tcgctctttg gcaccgatcg
     7321 tacgaagaga gcgaacggac gaaacaaccg gtgcgattcg aatatgtcca cccgcaaaaa
     7381 ggcagcgggg tcgaactgcg cctttccgcc accgtctgct ggattggaat aggaccaagc
     7441 gggcggtcgc gttatgctta cgtggtggac gatgtcacgg aagcacgatg cttggcagaa
     7501 gcgctgggcc agacggccga gcggtaccac ggggtggtga ctgcgctggc agaaggtgtc
     7561 gtcctgcatg atgagcaagg gaggattatc gcctgtaatc cgagcgcgga gcggattctt
     7621 gggctcacgg ccgaccagct gatggggcgg acgccggtcg atccgggatg gcagg
//
LOCUS       DAORRQ010000114         7662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_381_length_7662_cov_25.836861, whole genome shotgun sequence.
ACCESSION   DAORRQ010000114 DAORRQ010000000
VERSION     DAORRQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_7662_cov_25.836861"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PK224_19745"
     CDS             complement(<1..410)
                     /locus_tag="PK224_19745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB18038.1"
                     /translation="MKRVLEPELMDDAAQARAYAKADFAEENQGFVDRFREYFPDWAG
                     GHVVDLGCGPGDIPIRFLRSFPDARVTAVDASRPMLDLAAEAIAGSGVANRITLRCER
                     FQSLDLPEQADALLSNSRLSAPWLSAKAAPLSTPT"
     gene            complement(407..910)
                     /locus_tag="PK224_19750"
     CDS             complement(407..910)
                     /locus_tag="PK224_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRB18039.1"
                     /translation="MAQPVIPQVHQVSLSDGGVPKSAVLSAQVTCDGLVGDRQRNRKY
                     HGGPDRAVCLFSLELIDALRAEGHSIGPGSSGENLTLAGLDWSLIRPGDRLRIGEQVR
                     LEMTSYTTPCRYNAQWFKDGDYERIAQETHPGWSRLYARVLHEGTVRPGDPVVLESDD
                     LSGWGDR"
     gene            complement(918..1853)
                     /locus_tag="PK224_19755"
     CDS             complement(918..1853)
                     /locus_tag="PK224_19755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRB18040.1"
                     /translation="MASTSKADDCTADVSDTPGPGDWIQVDVQTSLDAGELLGVLNDP
                     DVTGAWQEQDCIHLYWPATRCGPDTWQGLRLALTRLGHPDPAGAITINRLADRDWNAQ
                     WSRLVEPIRVGRVVIRPSWKAVALNPDDIELIIDPKQAFGTGHHATTQLLIEWLQEVV
                     TPTDRVLDVGTGSGVLAMVALRLGASEAVGEDFDPVAIECARDYAQVNGFDARLTLAV
                     EYAQPQQSHHGFEPTLVLANLDRQTLLNAAETLCGYASGGARLLLSGLLIDQRAEIEA
                     AYAGHGVYVRASRERDGWVALEATGMESCDGGLCS"
     gene            1949..3775
                     /locus_tag="PK224_19760"
     CDS             1949..3775
                     /locus_tag="PK224_19760"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HRB18041.1"
                     /translation="MLRRAAQLKTNARRASARFTTWRLAIFAVGLFGSIIPHKLGWTL
                     LGNATLAFSFMLFLIVAWHHNRLEDRLHRLRRWQGIKQVHLARMRLGWADIPFRPVPV
                     PERHGYATDLDLAGPHSLLHLINNTVSSQGQERLASWLFDQPPEPAQWAGRQALIREL
                     TPLSLLRDRLSLEAQAVEDADIDGRRLLAVLQKRVDSPSFMPMLLTETFLALATAGLM
                     LTDLVYGIGNYWMLSFGLYAFLFLSVRSRSEEIFDHAQSLHRQLERLSAVIGYLERRS
                     YRAAPRLAELCRPLLQQDTSPSTSLKQAASVMNRLSVRAHPLVHYLINAFGPWDLYHT
                     YRLQHLQDRIAAVAPQWFELLAELDAAASLATFAYLHPDYPWPSLQQPDDAAHLNGAG
                     PILDAQGLAHPLIPAKARVANHIVFQERGRMFLVTGSNMSGKSTFLRTIGINTCLAQA
                     GGPVCAASFRWSLVRIGSCIRVDDSLDGGLSFFYAEVKRLKKILDSTRDMNGPPVLWL
                     IDEVFKGTNNRERLIGGRALIAALAGGNGFGLVTTHDLELAELERQLPTVTNVHFQET
                     VAKGALHFDYRLKPGPCPTTNALRIMALEGLPVESPPSTTSR"
     gene            3958..5460
                     /locus_tag="PK224_19765"
                     /pseudo
     CDS             3958..5460
                     /locus_tag="PK224_19765"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5771..7303
                     /locus_tag="PK224_19770"
     CDS             5771..7303
                     /locus_tag="PK224_19770"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRB18042.1"
                     /translation="MADATKPHVSSLQALIGAIALARALKTHWQGQQNVGLLLPPTVA
                     GALTTVAATLAGRTCVNLNYTVGKAGLESAIRQAHLGTIVTSRKFIEKAKLELPEGPT
                     ILWLEDIGATIGTRDKLTAAVLAVLAPLRLLESACGQTERVTMDHLATIIFSSGSTGE
                     PKGVMLSHFSIDANVQAVSQVLPLAEDDRILGILPLFHSFGYLVFWYVTLNGAATVFH
                     PSPLDVTAIGELCAKHRLTFLVCTPTFLQLYQRRCTPEQFSTLRVVLTGAEKLPLRLC
                     ESFQDRFGIGPIEGYGVTECAPVIAVNCPDFRAAGFFQPASRRGTVGQPLPGVSIRIV
                     DPDSFAILPPGTAGMLLVKGPNVMNGYLGREDLTAQAMRGGWYITGDIATLDDDGFLT
                     ITDRLSRFSKIGGEMVPHRLVEEALQLASGEELQICAVTGVPDERKGEQLAVLHTLPE
                     ERIPQILAKAAAGGLPNLFLPSRTHCIKVEALPILGTGKLDLRALKRIAMERLGEREG
                     SS"
     gene            7303..>7662
                     /locus_tag="PK224_19775"
     CDS             7303..>7662
                     /locus_tag="PK224_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18043.1"
                     /translation="MHEQRDPPPKATPQGQTLIDCHVHLAALPDGDNGCYISPKTLKS
                     PLFRFLLWKHGLSPDKPREANHKYLADLLTELKASRHVHKAVLLGMDGAYDHNGRYNQ
                     AHTDFLIGNDYVLKTAQA"
BASE COUNT         1404 a         2633 c         2161 g         1464 t
ORIGIN      
        1 gtaggtgtgc tgagcggtgc cgcctttgcg gagagccaag gtgccgacag ccggctgttg
       61 gacagcagtg catcggcctg ttccggcaag tcgagcgatt gaaaccgctc acaacgaaga
      121 gtgatgcgat tcgccactcc cgaaccggcg atcgcctccg ccgccaaatc cagcatggga
      181 cggctggcat ccacggccgt cactcgggcg tccgggaagg agcggagaaa acgaatggga
      241 atatctcccg gtccgcatcc cagatcgacg acatgaccgc cggcccaatc gggaaagtat
      301 tcgcgaaaac gatcgacgaa accctgattc tcctcagcga aatccgcctt cgcataggca
      361 cgggcctgcg ccgcatcgtc catcagctcc ggttccagca cgcgcttcat cgatcacccc
      421 acccgcttag gtcgtccgat tccagcacaa caggatctcc cggccgcacc gtcccttcgt
      481 gaagaacccg cgcatagagc cggctccatc cgggatgcgt ctcctgggca attcgttcat
      541 agtccccgtc cttgaaccac tgcgcattgt atcgacaggg cgtcgtataa ctcgtcatct
      601 ccagccgcac ctgctcaccg attcgaagtc gatcaccggg ccggatgagt gaccagtcga
      661 gccccgccag agtgagattc tccccggacg aaccaggacc gatcgaatga ccttcagccc
      721 gaagggcgtc gatcaactcc agggaaaaga ggcagaccgc ccgatccgga ccgccgtgat
      781 atttccggtt gcgctgacga tccccgacca atccgtcaca cgtcacctgc gcagacagta
      841 ccgccgactt cggcaccccg ccgtcagaca gactcacctg atgcacctgt ggaatgaccg
      901 gttgtgccat ctcggtatca ggagcagagc ccgccgtcac aggattccat gccggtggcc
      961 tccagggcga cccatccgtc acgttctctc gatgccctca cgtagactcc atgcccggca
     1021 taggcggctt cgatctccgc ccgctgatcg atcagcagac cggacagcag caggcgcgcg
     1081 ccgcccgacg catatccaca gagcgtctcc gcggcattca gcagggtctg ccgatccaaa
     1141 ttcgcgagga ccagagtggg ctcgaatccg tggtgagatt gctgcggctg agcatattcc
     1201 accgccaatg tcagccgcgc atcgaacccg ttgacctgag cgtagtcccg cgcgcactca
     1261 atcgccaccg gatcgaaatc ctccccgact gcctccgagg ccccgagccg caacgccacc
     1321 atcgccaaca cgccgctccc ggttcctaca tccagcacgc gatcggtggg cgtcaccacc
     1381 tcttgcagcc attcgatcaa cagctgggtg gtcgcatggt ggccggtgcc gaacgcctgc
     1441 ttgggatcga tgatcaattc gatgtcatcg ggattcagcg ccaccgcttt ccaacttggc
     1501 ctgatgacca cccgccccac cctgatcggc tccaccagac gcgaccactg ggcgttccag
     1561 tcgcggtccg ccaggcggtt gatggtaatc gcgccggccg ggtcagggtg cccgagccgg
     1621 gtcagtgcaa gcctgaggcc ctgccaggta tccggtccgc aacgggtcgc gggccagtag
     1681 agatggatac agtcctgttc ctgccaggct cccgtcacat ccggatcgtt cagcaccccc
     1741 aggagttcac cggcatcgag cgaggtctgc acatcgactt gaatccagtc gcccggacca
     1801 ggcgtgtcgg acacatcagc ggtacaatca tctgccttcg aggttgacgc catctcagac
     1861 tcccagtatc gtcagcggta cgatgaaacc tgatacagaa tccacaccat cgcgaaggtt
     1921 gcccacacgg gaacacagtc tcgtacgcat gctgcgtcgc gcggcacagc taaagacaaa
     1981 tgcccgtcgg gccagcgcaa ggtttacgac ctggcggctc gcaatctttg ccgtcggact
     2041 tttcggctct atcattccgc ataaactggg atggaccctg ctcggcaacg ccaccctcgc
     2101 gttcagcttc atgctgttcc tcattgtggc ttggcatcat aacaggctcg aagatcgctt
     2161 gcaccggctc cgccgctggc agggcatcaa gcaggtgcac ctcgcgcgca tgcgtctggg
     2221 ctgggccgac attccatttc gcccggtacc cgtgcctgaa cgacacgggt acgcgaccga
     2281 cctcgacctc gccgggcccc attcactcct gcacctcatc aacaacaccg tctcgtcgca
     2341 gggccaggaa cggctcgctt cgtggctgtt cgatcaaccg ccggaacctg cgcagtgggc
     2401 cggccggcag gctctcatca gggaattgac gccgctttcc ctgctcaggg accgattatc
     2461 attggaagcg caggcggttg aagacgccga tatcgacggc cggcggctcc tcgcagtgct
     2521 gcagaagcgg gtcgacagtc ccagcttcat gcccatgcta ctgaccgaaa ccttcctcgc
     2581 ccttgccacg gctgggctca tgctgacgga tctcgtgtac ggaatcggca actattggat
     2641 gttgtcgttc ggtctctatg cgttcctgtt tctctccgtc cgcagccgct cggaagaaat
     2701 cttcgaccat gcccaatcac tgcaccggca actggagcgg ctcagcgccg tcataggtta
     2761 tctggaacga cgatcatacc gtgccgcccc ccggttggcg gaactgtgtc ggccgctcct
     2821 gcaacaggac accagcccct ccacctcgct gaaacaggcc gcctccgtga tgaaccggct
     2881 cagcgttcgg gcccatccgc tggtgcatta cctcatcaac gccttcggtc cctgggacct
     2941 ctaccacacc tatcgcctcc agcacctaca agataggatt gccgccgtcg cgccgcaatg
     3001 gttcgagctg ctggccgaat tggacgccgc cgcctctctg gcgacgttcg cctacctgca
     3061 cccggactac ccctggccct cgctgcagca gccggatgac gccgcccatc tcaacggtgc
     3121 cggaccgatc ctcgatgcgc agggactggc gcacccgctc atccccgcga aggcccgcgt
     3181 ggccaatcac attgtgtttc aggaacgcgg gcgcatgttc ctggtgaccg gctccaacat
     3241 gtccggcaag agcacctttc tccgcaccat cggcatcaac acctgccttg cgcaggccgg
     3301 gggcccggtc tgtgccgcct cgttccgttg gtcgctggtg cgcatcggca gctgtattcg
     3361 agtggacgat tccctcgacg gaggcctctc ctttttttat gccgaggtga aacgactcaa
     3421 gaaaattctc gacagcacca gggacatgaa cggaccgcct gtgctctggt tgatcgatga
     3481 agtcttcaaa ggcaccaaca accgggagcg actcatcgga ggacgcgccc tcatcgcggc
     3541 cctcgcggga ggaaacgggt ttggcctcgt caccacccac gacctcgaac tggctgagct
     3601 ggagcgccaa ctccctaccg tcaccaacgt gcattttcag gaaaccgtgg cgaaaggcgc
     3661 actgcacttt gattatcgac tgaaaccggg tccctgcccc accaccaatg cgctgcgaat
     3721 catggcactc gaagggctgc cggtggaaag tccaccctcg acgacatcac gatagcgcat
     3781 tgcatcagtt caataatttc ggcagactga tcctcgattc cggacacgaa cgacggcaca
     3841 tagacacagg aggcacccat cgacccccag cccgactcct catcgacccg acttcccttg
     3901 cgcggactcc tgatcgcaca gttctgcggc gccttcaacg acaacgcctg gaaattcatg
     3961 gtcgcgctcc tgggtatccg ggcagccaca gccgcactcg cgccgggcca ggatctggaa
     4021 accgcatctc agacccagac gaccctcgcc atggtcgtgt tcaccctgcc gctggtcctg
     4081 acgtcattcg tggccggatt ctttgccgac cgtatcagca agcgcaccgt catcatcacg
     4141 atgaaggcgg tggaggtgct gctcatggcc gccgcaaccc tcgcgctcct ggcaaacccg
     4201 accggagggc tctgggcact cgtcgtgctc gcctgcatgg gcgtccacag cgcgatcttc
     4261 agtccggcca aatacggcat tcttcccgaa ctgctcccgc acgaacaact cgcgcgaggc
     4321 aacagcatcc tggagctgtt caccttcctg gcgatcctga ccggcaccac cgccggaggt
     4381 ttcttactgg ccggggcagg aacacaaccc tggctggccc ccttcgcgtt gaccctgctg
     4441 gccctgggcg gattcgcggc agcctgggcc gtaccgccgg tggcgccggc acgcgatgcg
     4501 ggcggattgt tcgacacctt gcgcggagcc ttgtcggcat tgcaggcgga tcggctgctg
     4561 cgtctcgcca tcaccggcgc ggtcttcttc tggaccatcg ccagcctcgt cgtccagaac
     4621 gtattggtgt atgcgaaagc ggtgctgggc ctctcggatg ccttggcgac cgttccctcc
     4681 gccttgatct cagtgggtat cggactgggc gcgctcgtcg tcggccgcct ctcccgttcc
     4741 cgcgtggaat acggactggt acccctggga gcggccggcg tcgccacttt cctcctgatt
     4801 ctcggctggt ggacgcccgc gttcagcggc acgctggcgc tcatgatcgc gctaggcatg
     4861 tcgagtgcgc tcatcttcat ccccatcaat gccctggtgc agtggcgcgc gcctcacgac
     4921 cggcggggat cggtcatcgc cctcgaaaac atctgtgtct ttaccggtat cctgctgggg
     4981 tcgttgagtg gcggtatcct ggccaacgta ggcctttcga ccgtcggcat tttcctcgcc
     5041 gctgccctcg tcacgaccat tggaacaacc tgggcgatgt ggctcatgcc ggagaccttt
     5101 ctgcgattgg tcctcgtcct gctgaccaat accatctacc ggttgcgcat cgtcggacag
     5161 cagcaggtgc cggtgacggg cggcgccctt ctggtcccca atcacatctc cttcatcgac
     5221 ggctttctgc tgatcgcgag cctggatcgt cccatccggt tcgttgtgga tgcccagtac
     5281 gtcaatcacc ggctcttcaa gccgttcatg cgggcgttgc aggtcatccc gatttcatcg
     5341 gctggcggtc cccgcgcgat tctccgcgcc ctgcgcgagg cgggtcatgc gcttgatgcc
     5401 ggtgaccttg tctgcatttt ccctgaaggc caaattaccc gcaccggaaa cctcctcccg
     5461 tttcgccgcg gattcgaacg gatcgtcaaa ggacgggcgg ttccggtcct accggttcac
     5521 ctggatcgcg tctggggcag cattttcagt tttgtcgggg gggcgattcg tcaccaaatg
     5581 gccggagcgt gttccttatc cggtcaccgt ttcgttcggg actccggttc ccgccgagac
     5641 gccggcgcac gaactccgcc gcctcgtccg cgaactcggg gaagctgcct ggcagttgcg
     5701 gaaaccgacc cggcgcccgt tgcaccaggc cttcatcagc accatgcggc ggcatccctt
     5761 ccgtctcgca atggccgatg cgacgaaacc ccacgtgtct tcgctccagg ccttgatcgg
     5821 ggcgatcgcc ctggcacgcg ccctgaaaac ccactggcag ggccaacaaa atgtgggcct
     5881 gctgctgccg ccgaccgtgg caggagcgct gacgacagtc gccgccacac tcgcgggccg
     5941 cacctgcgtc aacctcaact acaccgtggg caaagccggg ctggaatcgg ccatccgcca
     6001 ggcgcacctg ggcaccatcg tcaccagccg taagtttatc gagaaggcca aactcgaact
     6061 cccggaaggc cccaccattc tctggctgga ggacatcggc gccaccatcg gcacgcggga
     6121 caaactcacc gccgcagtcc tcgctgtact cgcgcccctc cgtcttctgg aatcggcctg
     6181 cggacagacc gagcgcgtga ccatggatca cctcgccacc atcatcttca gcagcggcag
     6241 caccggcgag ccgaaaggcg tgatgctgtc tcacttcagc atcgacgcga atgtgcaggc
     6301 cgtctcgcag gtgctccccc tggccgagga tgatcgaatc ctgggcattc ttccgctctt
     6361 ccactcgttc ggctatctcg tgttctggta cgtcacgctc aacggcgctg cgaccgtgtt
     6421 tcatccttca ccgctcgatg tgacggcgat cggtgaactc tgtgccaaac accgcctcac
     6481 atttctggtt tgcaccccca cttttctgca gctctatcaa cggcgctgca cgccggagca
     6541 gttcagcacg ctacgagtcg ttctcacggg ggcggagaaa ttgccgcttc gcctttgcga
     6601 atcgttccaa gaccgcttcg gtatcggccc gatcgagggc tatggcgtga ccgaatgtgc
     6661 gccggtcatc gccgtgaact gcccggactt ccgggctgcg ggatttttcc aaccggcctc
     6721 gcggcgtggc accgtcggcc aaccgctgcc cggcgtctcc atccgtatcg tcgatccgga
     6781 cagctttgcc atcctcccgc ccggcacggc aggcatgctg ctcgtcaaag ggccgaacgt
     6841 gatgaacggg tatctcggtc gcgaggattt gacggcccag gccatgcgcg gcggatggta
     6901 tattaccggt gacattgcca cgctcgacga cgatgggttt ttgaccatca ccgatcggct
     6961 ctcacgtttt tcgaagatcg gcggtgaaat ggtgccgcat cggctggtgg aagaggcgct
     7021 ccaactagca tccggcgagg aactacaaat ctgcgccgtc accggcgtac cggatgagcg
     7081 taaaggtgag cagttagctg tattgcatac gcttcctgaa gagaggatcc cccagatcct
     7141 tgccaaggcc gcagccggtg gcctcccgaa tctatttctg ccctcgcgca cccactgcat
     7201 taaagtcgag gcactcccga tcctgggaac gggcaaactg gatctcagag ccctcaagcg
     7261 gatcgcgatg gaacggctgg gggagcgtga aggctcatcg tgatgcacga acaacgagat
     7321 ccgcccccga aggccacgcc gcagggacag acgctgatcg actgccatgt ccatctggcc
     7381 gcgctgccgg acggcgacaa cggttgctat atttcaccca agacgctgaa gagcccgctg
     7441 ttccggtttc tgctgtggaa acacgggctc tcaccggata agccacgcga ggcgaatcac
     7501 aaatatcttg ccgacctcct gacggagctg aaggcctcac gccacgtcca caaggccgtg
     7561 ctgctcggca tggacggcgc gtatgaccac aacgggcgtt acaatcaggc acacaccgac
     7621 tttctcatcg gcaacgacta tgtcctcaag accgcccaag cc
//
LOCUS       DAORRQ010000115         7529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_385_length_7529_cov_32.231870, whole genome shotgun sequence.
ACCESSION   DAORRQ010000115 DAORRQ010000000
VERSION     DAORRQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_385_length_7529_cov_32.231870"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>7529
                     /locus_tag="PK224_19780"
     CDS             <1..>7529
                     /locus_tag="PK224_19780"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRB18044.1"
                     /translation="TIEQLVQSETQQQAIDQVSMSNSIGSLRFLAAMDWREFVEAQSL
                     VEQTFHEDPAGVYGQMDFATRDRYRHAVEKLAKSSPLSEGDVARQAILLAQTGAAAEG
                     PYHRTAHVGFYLIDNGQTQLEQAVSLRRSAFDRLRRWTGSSSVPIYLGSILFLTATFT
                     GCFLALAIAAGWNGWLLALLGFVSALAASQLAVTLVNWIATALVTPYPLPRMDFSDGL
                     PPALRTLVVIPTLLQDVRGIDDLLEALEVRFLANRGSHLHFALLTDFQDADQETVAGD
                     DQLVLAAGAGIDALNAKYAGEDGDAFFLFHRPRRWNAHERLWMGYERKRGKLADLNGL
                     LRGGSTDRFSTIVGQPDILSTVKYVITLDTDTQLPRDSARQFVAAMAHPLNRPQYDPA
                     AQRVTAGYGILQPRVAVSLPGTNRSRYARLNAGEPGIDPYTRAVSDVYQDLFGEGSFI
                     GKGIYEVDAFEQALSDRFPENRILSHDLLEGSYARAGLLSDVQLYEEYPARYNTDVVR
                     RYRWIRGDWQIARWAFPRVPGPNGHTRSNPLSTLSRWKIFDNLRRSLVPATLTSLFVV
                     GWTLLSPVWLWTLATLSLFLIAPLLGAVVDLCRKPEDMRMSQHLTATARGMTQQLTQA
                     LLTLTCLPYESFYSLDAIVRTAGRVWFNRAGLLEWNPSGSADRSRTDLIGSYRSMWIG
                     PAMALIITIILMQTRAEALLIAAPVLSLWALSPLFTWWISRPLARREARLTADQTMFL
                     RKMARKTWAFFETYVSPEDHWLPPDNYQEHPTPKVAHRTSPTNIGLALLANLSAYDFG
                     YLSAGQLIERTAHTFDSMATLERFRGHFYNWYDTQTLKPLLPMYISSVDSGNLAGHVM
                     TLHSGLLSLPEHKILAERTFEGLRDTLALLSEALGTPTSQVDALQKNLVAACGNRPTT
                     LSEAHHTFSLLTRQVDEVTAHLDPATNAEAHRWAHAFARQCRDAHNDLMLLAPWIGLP
                     LTAHTLSLFPELDQIPTLRTLTRLEGEWLPAIDARLGPDASGTERTWLIELRRHLSAA
                     SRLAEQRLASLDHLARQANQFAQMEYDFLFDDTRFLLSIGYNVAERRRDASYYDLLAS
                     EARLCSFVAIAQGQLPQESWFALGRLLTTTGGEPILLSWSGSMFEYLMPLLVMPTYQQ
                     TLLDQTYRAAVKRQIEYGRERDIPWGVSESGYNMVDAQLNYQYRAFGVPGLGLKRGLG
                     EELVIAPYATSLALMVAPEEACLNLQRLTAEGADGPYGLYEAIDYTPSRLPRGQSRVI
                     IRSYMAHHVGMSFLSLAYLLLDRPMQKRFEADPLFQASTLVLQERIPKATAFYAHSTE
                     LSDLRTTSGTADTPVRVLTTANTPVPDVLLLSNGRYHVMVTNAGGGSSRWKDLAVTRW
                     REDSTCDNWGTFCYLRDVESGEFWSTAYQPTLKPSKTYEVIFSEGRAEFRRRDHEIET
                     HAEIVVSPEDDIELRRIRLTNCSGTTRTIDLTSYAEVVLALPAADALHPAFSNLFVQT
                     EIIRERQAILSTRRGRSQDEHAPWAFHLMAVRGPHSGEISYETDRLQFIGRGRTLAAP
                     QALDHSGPLSGSQGSVLDPIVSIRCPIVLAPEESITVDLVSGIGETRDLCLRLVDKYQ
                     DRRLADRVFELAWTHSQVVLRQLNTTEADAQLYGRLAGSILYANASLRAEAAVLSKNR
                     RGQSGLWGYAISGDLPIVLLQIGDPSNIDLVRQMVQAHAYWRLKGLAVDLVIWNEDHA
                     GYRQVLHDQIMGLISAGVEANVMDRPGGIFVRTAEQMSVEDRLLQQSVARVILTDRRG
                     TLADQITRLASPAAPAYRFKPTRTHRADPPATIEPRTDLLFYNGLGGFSSDGREYVIT
                     TTAEQVTPAPWVNVLANPNFGTVVSENGLAYTWSENAHEFRLTPWHNDPVTDSSGEAF
                     YLRDEESGHVWSPMPMPCRGTNPYVSRHGFGYSCFEHTEGGIRSEVWVYVALDASVKF
                     TVVKLRNESGRPRRLSATGYVEWVLGDLRPKSVMHVISEIDPATGALFARNPYNTDFP
                     GRIAFFDVDEGTRSMTGDRTEFLGRNGTLRNPASMSRSRLSGKVGAALDPCGAIQMPF
                     DLAVGQERDCTFRLGVGKDTEDARQLVRRFRGATARRAALETVWHHWTHTLGAVHVET
                     PDQSLNVLANGWLLYQTIACRLWARSGYYQSGGAFGFRDQLQDVMALVHAKPHLAREQ
                     LLLCAGRQFKEGDVQHWWHPPSNRGVRTRCSDDFLWLPYVTSRYVMTTGDTGVLDTPI
                     QFIEGRPINADEDSYYDLPGRSEQSGSLYDHCVRAILKGLTKGVHGLPLIGSGDWNDG
                     MNMVGDRGQGESIWLGFFLYDVLMRFAEVADTKGDATFAERCRTEAAQLQHRIEQQGW
                     DGEWYRRAYFDDGTPLGSATNDECQIDSIPQSWSVLSGAGDSARSRSGMEEVYRRLVR
                     RDDALVQLLDPPFDKSALNPGYIKGYVPGVRENGGQYTHGALWATMAFAALGDRERAW
                     ELFRMINPVNHAQSPGDINRYKVEPYVVA"
BASE COUNT         1448 a         2364 c         2250 g         1467 t
ORIGIN      
        1 cacgattgag caactggtgc agtcggaaac gcaacaacag gcgatcgatc aagtctccat
       61 gagcaacagc atcggcagtc tccggttcct ggcggcgatg gactggcgcg agtttgtcga
      121 agcgcagagc ctggtggagc agacgttcca tgaagatccg gccggcgtgt acgggcagat
      181 ggattttgcc actcgtgacc ggtatcgcca tgccgtggag aaactcgcaa agagtagccc
      241 cctgtcggaa ggtgacgtcg ctcgccaggc gatcctgctc gcgcagacag gggccgctgc
      301 agagggaccg taccaccgca ccgcccacgt cggcttctat ctcatcgaca acggacagac
      361 gcaattggaa caggccgtga gcctgcgccg ttctgccttc gacaggctcc ggagatggac
      421 cggatccagc agcgtaccaa tctatctcgg ctccattctg ttcctcacag ccacattcac
      481 tggatgtttc ctggccctcg ccattgcagc aggttggaat ggatggctgc ttgccctgct
      541 gggattcgtg tcggccttgg ctgccagtca gctggcggtg acgctggtga actggatcgc
      601 cacggcgcta gtgaccccgt accccctccc gcgaatggat ttttccgatg gccttccccc
      661 ggcgttacga acgttggttg tgattcccac actcctccaa gatgtccggg gtatcgacga
      721 cctccttgag gccctcgagg tccgattcct cgccaaccgg ggcagtcatc tacactttgc
      781 cctgctcacg gatttccagg atgccgacca agagacggtg gcaggtgacg atcagttggt
      841 gctggcggcc ggagcaggca tcgatgcgct gaatgccaaa tatgcaggcg aggatggcga
      901 cgcgtttttt ctcttccatc gcccgcgtcg ctggaatgcc catgagcggc tctggatggg
      961 atacgagcgg aagcgaggca aactggcgga cttgaacggg ctcctgcgtg gcgggtcgac
     1021 ggatcggttt tccacgatcg tcggacaacc ggacatcctg tccacggtga aatatgtgat
     1081 cacgctcgat acggataccc agttgcctcg cgattccgcg cggcaattcg tggccgccat
     1141 ggcccatccg ctcaatcgtc cgcaatacga tcctgcggca cagcgcgtga ctgcggggta
     1201 cggaattctg caacctcggg tggccgtcag cctccccggc accaaccgct cgcgttacgc
     1261 ccggctcaac gccggcgaac ccggcatcga tccctacaca cgcgccgtgt cggacgtgta
     1321 tcaggacctg tttggagaag ggtccttcat cggcaagggg atctatgagg tcgatgcctt
     1381 cgagcaggcg ctcagcgatc gcttcccgga gaatcgcatc ctcagccacg acttgcttga
     1441 aggctcctat gcccgcgccg gactcttgag cgacgtgcag ctctacgaag aatatcctgc
     1501 ccgctacaac acggacgtgg tccggcgtta tcgctggatc cgcggagatt ggcagattgc
     1561 gcgctgggcc ttcccccgcg tgccgggacc gaacggacat acccgcagca atccgctgtc
     1621 caccctgtcg cgttggaaaa ttttcgacaa cctgcgacgc agcctcgtcc ccgctacgct
     1681 gacctcctta ttcgtagtgg gatggactct gctttcccct gtctggctct ggaccctcgc
     1741 cacgctcagc ctgttcctga tcgcgccgtt actgggcgcc gtcgtcgatc tgtgccgcaa
     1801 gcccgaggat atgcggatga gccagcacct caccgcaacc gcacggggca tgacacaaca
     1861 gctgacccag gcgctcctca cgctcacgtg cctgccttac gaaagcttct acagcctgga
     1921 cgcgatcgta cggaccgccg gacgtgtatg gttcaatcgc gctggtttat tggaatggaa
     1981 cccgtcaggc tcggcggaca gaagcaggac ggacctgatt ggttcctacc gatcgatgtg
     2041 gattggtccg gccatggctc tgatcatcac gatcattctg atgcagactc gagccgaagc
     2101 gctcctgatt gcggcgccgg ttctcagcct gtgggcgctg tctcccctgt tcacctggtg
     2161 gatcagcaga ccgctcgctc gacgcgaggc cagactcacg gccgaccaga cgatgtttct
     2221 ccggaaaatg gcccgcaaga cctgggcctt tttcgagacc tatgtcagcc cggaggatca
     2281 ttggctcccg cccgataact atcaggagca tccgaccccg aaggtggcgc atcgaacgtc
     2341 accgacgaat atagggttgg cgctgttggc caatctctcc gcctacgact tcggttacct
     2401 gtcggcagga cagctgatcg aacgcaccgc tcataccttc gacagcatgg caaccttgga
     2461 gcggtttcga ggccatttct acaattggta cgacacccag acgttgaaac cgctgctgcc
     2521 catgtatatt tcgtcggtgg atagtggaaa cctcgcgggg catgtcatga ccctgcactc
     2581 ggggctcctc agcctgccgg aacacaagat tctcgctgag cgcacctttg aaggactccg
     2641 cgataccctc gcgctgctga gcgaggcgct cggaactcca acctctcagg ttgacgcgct
     2701 tcagaagaac ctggtcgcgg cctgtggcaa caggccgacc acactctcgg aagcccatca
     2761 cactttctcc ctgctcacca ggcaggtgga tgaagtcacc gcccatctcg atcccgccac
     2821 caacgcggag gcgcaccgat gggcccatgc gttcgcaagg caatgccggg acgcgcataa
     2881 cgacttgatg cttctggctc cctggatcgg ccttcctctc accgctcaca ccctgagcct
     2941 gttcccggaa ttggatcaga tccccacgct ccggacgctg accagactgg aaggcgaatg
     3001 gctgccggcg attgacgcgc gacttggacc agacgcatcc ggcacggagc ggacctggct
     3061 gatcgaactt cgacggcacc tgagcgcggc cagtcgactg gccgaacaac gcctggcctc
     3121 gctcgaccac ttggcccggc aagcgaatca atttgcccag atggagtacg acttcctgtt
     3181 cgacgacacg cggttccttc tctccatcgg gtacaacgtc gcggaacgac gacgggacgc
     3241 gagttactac gacctcctgg cctccgaagc gcgattgtgc agcttcgtgg cgattgcgca
     3301 gggccaactg ccgcaggaga gctggttcgc cctcggccgt ctgctcacga cgacgggagg
     3361 agagccgatt ctcctgtcct ggagcggatc gatgttcgaa tacctgatgc cgctgctcgt
     3421 catgccgacg taccagcaga cgctgttgga tcagacctac cgcgcagcgg tgaagcgaca
     3481 gatcgaatac ggccgggagc gagatattcc ctggggtgtc tcggaatccg gctacaacat
     3541 ggtcgacgcg cagttgaact atcagtatcg ggccttcggt gtgcccggcc ttggactgaa
     3601 gcgaggcctg ggcgaggaat tggtcattgc accctacgcc acctccctcg ccctgatggt
     3661 cgcgccggaa gaggcttgtc tgaatctcca gcgactcacg gccgaaggcg ccgatggacc
     3721 atacggcttg tacgaagcga tcgattacac gccctcgcgt cttccccgcg gacaatcgcg
     3781 tgtcatcatt cgctcctaca tggcccacca tgtggggatg agtttcctct ccttggccta
     3841 tctgctcctc gaccgcccga tgcagaagcg cttcgaagcc gatccgctgt ttcaggccag
     3901 cacgctggtg ctccaagaac gaattcccaa ggccacagcc ttctatgccc acagcactga
     3961 gctgtcggat ttgcgcacga cgtccggtac ggcggacacg ccggtgcgcg tcctgacgac
     4021 cgcgaacact ccggtgccgg acgtgctcct cctgtcgaac ggccgatacc acgtgatggt
     4081 gacgaacgcg ggaggcgggt ccagccgatg gaaggacctg gccgtcacgc gttggcgtga
     4141 agacagcacc tgcgacaact gggggacgtt ttgttatctc cgcgacgtag agagcgggga
     4201 attctggtcc acggcctatc agccgacact gaaaccgtcc aagacctacg aagtcatctt
     4261 ttccgaaggg cgcgccgagt ttcgtcgccg cgatcacgag atcgagacac atgccgaaat
     4321 cgtcgtgtcg ccggaagacg acatcgaatt acggcggatc cgtctcacca actgttccgg
     4381 cacgacacgc acgatcgacc tcaccagtta tgcggaggtc gtgctggcgc tgcccgcggc
     4441 cgatgccctc catccggcct tcagtaatct tttcgtccag acggaaatca tccgcgaacg
     4501 tcaggccatt ctctccacgc gccgtggccg ttcgcaggat gaacatgccc cctgggcatt
     4561 ccacctcatg gcggtacgtg gcccgcacag cggggagatt tcctacgaaa cagaccgcct
     4621 gcagttcatc ggccgggggc gcaccctcgc cgctccgcag gctctggacc attccggccc
     4681 gctttcagga agtcagggct ccgtgctgga ccccattgtg tccatccgtt gtccgatcgt
     4741 cctcgcacct gaagagtcga tcaccgtgga tctggtgtcg ggcatcggag agacccgcga
     4801 cctgtgcctg cgtctggtcg acaagtatca ggaccggcgt ctcgccgatc gggtgtttga
     4861 gctggcctgg acccatagcc aggtcgtgct gcgccagctc aatacgaccg aagcggatgc
     4921 gcaattgtat ggcagactgg ccggctcgat cctctatgcg aatgcctcac tcagggcaga
     4981 agcggccgtg ctgagcaaaa accgccgcgg acaatcgggc ctctggggct atgcgatttc
     5041 gggggacctg ccgatcgtac tgctccagat cggcgacccg tccaacatcg atctggtccg
     5101 tcaaatggtc caggcccatg cctattggcg actgaagggg ctggccgtgg acctcgtgat
     5161 ttggaacgaa gatcacgccg gctatcgtca ggtgttgcac gatcaaatca tggggctgat
     5221 ctcggccgga gtcgaggcca atgtgatgga tcgaccgggc ggtatcttcg tgcgaacggc
     5281 ggaacagatg tcggtcgaag accgcctgct gcagcaatcg gtggcccgcg taatcctcac
     5341 cgatcgacgc ggaacgctgg cggaccagat tacacgcctc gcctcccctg cggccccggc
     5401 ctaccgcttc aaaccgacgc gtacccatcg cgccgatccg ccggccacca tcgagccccg
     5461 aacagatctg ctgttctata acggtctcgg cggattttct tccgatggcc gcgagtacgt
     5521 gatcacgacc acagccgaac aggtaacgcc cgctccctgg gtcaatgtgc tggcgaaccc
     5581 gaatttcggc accgtcgtct cagagaacgg cctcgcttac acctggagcg aaaatgcgca
     5641 tgagttccgt ctcacaccct ggcacaacga tccggtgacc gattcgagcg gagaagcctt
     5701 ctatctccgt gatgaggaga gcggccacgt ctggtcgccg atgccgatgc cctgcagggg
     5761 tacgaacccc tatgtcagtc ggcatggatt cgggtacagc tgctttgagc atacggaagg
     5821 cggcattcgc tcagaggtct gggtctatgt ggcgctggat gcctctgtaa aatttacggt
     5881 ggtgaagctg cgcaacgaat cgggccgacc acgacgcctg tcggcgaccg gctatgtgga
     5941 atgggtgctg ggtgatctgc ggccgaaatc agtcatgcat gtgatcagtg aaatagaccc
     6001 ggccaccggg gcgctgttcg cacggaatcc ctacaacacg gatttccccg ggcggattgc
     6061 tttctttgat gtggacgagg gcactcgaag catgaccggc gatcgaaccg aatttctcgg
     6121 acgcaacggc accttgcgca atcctgcgtc gatgagtcgg tcacgactgt ccgggaaagt
     6181 gggggccgct ctggatccgt gtggcgcgat tcagatgccc ttcgacctcg ccgtcggtca
     6241 agagcgcgac tgtaccttcc ggttgggagt tggaaaagat actgaggatg cccggcagct
     6301 cgttcgtcgc ttcagagggg cgacggcacg ccgtgcggcg ctggaaaccg tttggcatca
     6361 ttggacacat acgttgggag cggtccatgt ggagaccccc gatcagtcgc tcaatgtgct
     6421 tgccaatggc tggctgctgt atcaaacgat cgcctgccgt ctttgggcgc gcagcggcta
     6481 ctatcaatcg ggaggcgcgt ttggattccg cgatcagttg caagacgtga tggccttggt
     6541 ccacgcgaaa ccgcatctcg cgagagaaca gctactgctg tgcgccggcc gtcagttcaa
     6601 ggaaggagac gtgcagcatt ggtggcatcc accgtcaaac cggggcgtgc gcacgcgctg
     6661 ctccgatgac tttctctggc ttccctatgt caccagtcgc tacgtgatga caaccgggga
     6721 caccggcgtg ctggacaccc caatccagtt catcgaaggt cgcccgatca atgccgacga
     6781 agattcctac tacgatcttc ccggccggtc ggagcaatca ggcagtctat acgaccactg
     6841 tgtccgggcc atcctgaagg ggctcacgaa gggtgtacac ggtctgccgc tcatcggttc
     6901 aggtgattgg aacgacggga tgaatatggt cggcgatcgc ggccagggag aaagcatctg
     6961 gttgggattc ttcctctatg acgtcctcat gcggtttgct gaggtggcgg acaccaaagg
     7021 tgacgcgacg ttcgcggagc gctgccggac ggaagccgcg cagttgcaac accggatcga
     7081 acagcagggc tgggacggag agtggtaccg tcgcgcctac ttcgacgacg gcacaccgct
     7141 cggatcggcc accaatgacg aatgccagat cgattccatc ccccaaagct ggtctgtcct
     7201 gtcgggcgcg ggagattcgg cccgttcacg atcgggcatg gaggaagtct atcgacggct
     7261 ggttcggcga gacgatgccc tcgtccaact gctggacccg ccgttcgaca agtccgcatt
     7321 gaatcccggc tacatcaagg gctacgtgcc gggagtcaga gagaatggag gacagtacac
     7381 ccatggcgcg ctctgggcca ccatggcctt tgccgcactg ggcgaccgcg aacgcgcctg
     7441 ggaactgttt cgcatgatca acccggtgaa ccatgcccag tcacccgggg atatcaaccg
     7501 gtataaagtc gaaccctacg tggtagcgg
//
LOCUS       DAORRQ010000116         7483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_389_length_7483_cov_10.615240, whole genome shotgun sequence.
ACCESSION   DAORRQ010000116 DAORRQ010000000
VERSION     DAORRQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_389_length_7483_cov_10.615240"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PK224_19785"
     CDS             complement(<1..490)
                     /locus_tag="PK224_19785"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18045.1"
                     /translation="MVSIFPLITIFAPLLGTLLISLFWRDLGDRVARLGIAALWCSAL
                     TSLATLAVVLSGDTIQLALIGAQSGAMTYTVMVDRLAAVMMALISLVSLIIHVYSERY
                     MVGDAGYVRFFALLGGLTCVLLSLVSSGNLFWLFVCWHLVTWLLARLLVCNPDSRAAR
                     DAG"
     gene            964..1449
                     /locus_tag="PK224_19790"
     CDS             964..1449
                     /locus_tag="PK224_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18046.1"
                     /translation="MRSTHSRFLRLDSLLRAWAVLWLLALPLFHIHPQTDPHHGEASH
                     IHAAAVHTVFSADLEGEFEDHHDAHHHAQDTDSEPVLSAQGPHTWENDHELGFSLLND
                     ATDRKLVKPLLAHLQFVRHNLLPVPEQRDQPAEQDPPILSNTILACDLPTRAPPSSLL
                     S"
     gene            1554..2813
                     /locus_tag="PK224_19795"
     CDS             1554..2813
                     /locus_tag="PK224_19795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRB18047.1"
                     /translation="MIARLVCSLVLAMAAAGPLAGVSVAWAEAPRTATYSLPDILTLA
                     VQHNPTLAGAEGVVKQSQGQQIAAGAYPNPSVSGSAGRGAIRDPSTGTRVTERTFTVE
                     QPLEWTGKRQARQEAADAGAAGATAAFEDTRLTLLADVKVAFYQLLFAQRDVDLAAQN
                     VTSVEEVLRTVHARVAAGEATSFDSMKAGVEVQKAKKEVARANSTLLVAKARLNTLTA
                     GSLGKEFSIQGDFQSPKPGVNAEALATQAMEQHPAVRRLSKLAEQAEHTLRYEREARV
                     PNISLLGSYHREAGDESVTAGLSMPLPLWYRRQGEIQSALGAKHRADAERLRQQNELE
                     QAITQHAQEVRTAQDQLQVFETGLLKQAEQTLTVARTSFRHGAASLLDVLDAQRVYRQ
                     TQLEYAQVRADLSIALARLERAVGASL"
     gene            2810..4021
                     /locus_tag="PK224_19800"
     CDS             2810..4021
                     /locus_tag="PK224_19800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB18048.1"
                     /translation="MSLLAWFAPRQETMIGRCATTGMILTILSVLPGCGEQGAEPPKP
                     PSPVEVAPQAAFTHAIHPPPAVRERLRTESATRRAVPELVTAPGEVALDLKKVAKITS
                     RIGGQVEHIHVQLGDRVKPAQPLAAIGSLQLDQLVEEYLVGKAQADVAENSLRRTEKL
                     RTDDIVPERKLIEDKGHYLETKARYQHVREKLLAMGISKAELAGLERGGHEEGHHYTL
                     TSPIAGTVVAQNAVRGQGVTPGAELFEVVDTSRVWVFANLPIEQARKFKEGDLGTITP
                     KGGEVITAPLTYLAPVADETTRTIQVRFEVENRQGQLKPHEYVEAALTLSGAPTVAVP
                     VSALTTIDKTRGVFLEGPDGYTFAPIDAGRESGGWVEIRTGVKEGNPVVIEGVFDLKN
                     VLLKEQIGSGE"
     gene            4029..7151
                     /locus_tag="PK224_19805"
     CDS             4029..7151
                     /locus_tag="PK224_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRB18049.1"
                     /translation="MNPLLTFSLRYRFFTLVAIAVVIATGIWSFTNLTIDAVPDLTPV
                     QVQVLTRAPALGPVEVEQFVTFPIEASLNGLPALRELRSVSRYGLSAVTAIFDDQTDI
                     YRARQFVTERLAQAMERIPAEYGRPVMGPLTTGLGEVYQFTVKGPGYSPMALRTLLQW
                     DIGMKLRAVPGVVEVNIWGGEPQQFHVIVDPSKLLSFKLTMKQIFDALQRNNAIAGGG
                     YIEHQREQLLIRGEALATQVSDLARIVVAHGSGGVPVYIADVAEVKEGSGLRIGAATA
                     MGEGETVIGMVQMLAGENAQQVVTRVKARVQEIQATLPSGVTIEPYYDRTIFVSKVMT
                     TVRNNLLEGGLLVIAVLFLFLGDLRAGLIVASAIPLAMLIAFTGMMQAGLSGNLMSLG
                     AIDFGLLVDGSVVMIDNILRRLADKPVRNQEERLVTVLAAGREVLRPMTLAVSIIILV
                     YVPILALTGIEGKMFQPMALTVIMALAGSLLLAVTVTPLLSFWFVRTGVNRQETLVIC
                     GLRDAYAPLLKWAMRRPVWPVSIAIILFIISLGAGTRLGIEFVPRLEEGDLALQVWRL
                     PSISLTESVATALEIERVLRRFPEVTQVVTRTGSPEVATDVMGVELSDVFIILKPQQE
                     WVTAGTRDGLIEAMKRRIEEQVPGVGLGFTQPIEMRFNELIAGVRSDLAVKIFGPDLD
                     ILKQQAERAARALQAVPGAADVKVEQVAGLPLLRVIVDRAAIARYGLTADDVLTLIQS
                     TRVGTVVGTVVQGSRRFELVVRLADRVSQDPASLGRLLIPTMHGELVPLSRVTSITVD
                     SGPAQVSREHVQRRIVVECNIRGRDLGGFVAEARQAVARAVTLPAGYEMHWGGQFEHL
                     QEAARRLTLVVPVTLLLILGMLSVIFGAMRPALLIFLNVPLALSGGILALWLRGLPLS
                     ISAIIGFIALFGIAVLNAVVLVSHIRRLEAEGASTGEAVVQGAMDRLRPVLMTALVAS
                     LGFLPMALATSMGAEVQRPLATVVIGGLLTSTALTLLVIPALYPYWVRDETMRPRPLT
                     DQAGMNETGA"
     gene            7352..>7483
                     /locus_tag="PK224_19810"
     CDS             7352..>7483
                     /locus_tag="PK224_19810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB18050.1"
                     /translation="MVIDDEPSIRGLLREVLERSGHKVVEAKDGREALDLYQKHKADL
                     "
BASE COUNT         1506 a         2316 c         2286 g         1375 t
ORIGIN      
        1 gccccgcatc cctggccgca cgactatcgg ggttgcacac cagcaacctc gccaataacc
       61 aggtcaccag gtgccagcag acaaacagcc agaacaggtt gccgctgctg accaggctca
      121 acaacacaca cgtgagtccc cccagcagcg cgaagaagcg gacatagccc gcatcgccca
      181 ccatgtaccg ctcggagtac acatgaatga tcaggctcac gagactgatg agcgccatca
      241 tcaccgctgc caagcgatcc accatgactg tataagtcat cgcgcctgac tgcgcgccga
      301 tcagggccaa ctgaatcgtg tctcccgaca gaactaccgc cagtgtggca agcgaggtca
      361 gggcggaaca ccagagcgcg gcaatgccga gacgcgcaac ccggtcgccc agatcacgcc
      421 agaacaaact gatcaggagc gtgccgagaa gcggcgcgaa gatcgtaatc agaggaaata
      481 tcgagaccat gggatcagac tctccgagaa acgcgtgatc tgtgcggggt catcagcaag
      541 aaaagagcct ggccgccgga tcgcccgaat actgggaatt ccatcgaatt tgaagcctct
      601 ccgcgtagag attcaacata ggggccggtt cgacctcacg accgacgtgc atcactgcac
      661 gaacgtgcat atatgcacag cttggttggc gaggtcaacg aaaggaagag agcatccgga
      721 agcgtatgcg tctcacggct aaatctgaga cgcggagaag acagaggcag tcagacgggc
      781 aatcactgat gaaaatcgtt ttctacggtt accacgcaaa aaagagaact tgccggaaag
      841 atctcaccgg tagcacacga ctttgttgcc cgttcgaatt ttgtttagaa ttcgcctcgt
      901 tccctgctcg tttcttgatg tgtttctctc cttgtgatag cgtccactta gaagcgtatg
      961 cacatgcgtt ccactcacag ccgattcctc cgactcgaca gcctgctccg cgcctgggcg
     1021 gtactgtggc tgttggcctt gccgctgttc catatccatc ctcagacgga tccccatcat
     1081 ggcgaagcca gtcacatcca cgcggctgcc gtgcacacgg tcttttcggc cgaccttgag
     1141 ggtgaattcg aagaccacca cgatgctcat caccatgcgc aagacacaga ttcggagcct
     1201 gttctttctg cgcaaggtcc gcatacctgg gagaacgatc acgagctggg cttttcgctg
     1261 ctgaacgatg ccaccgaccg caagctcgtg aagccgctgt tggcccatct gcagtttgtg
     1321 cgtcataacc tcctgcccgt tccggagcag cgtgatcagc ctgccgaaca ggaccctccg
     1381 atcctctcca acacaatcct cgcttgtgat cttcctacgc gcgcgccgcc atcctcactc
     1441 ctcagctaat cgtcgtccgt cctgacatcc tggattctga tcgcatagcg ctcgcagggc
     1501 gaggcctgtg cctcgcacca tcgcgagccg attgcgcacg gaggaggctg ctgatgattg
     1561 ctcgtcttgt ctgttccctg gtcctggcaa tggctgccgc cggccccctc gcgggcgttt
     1621 ccgtagcctg ggctgaagcg ccacgcaccg ccacctattc cttgccggat attctgacgc
     1681 tggcggtgca acacaacccc acactggccg gcgccgaggg ggtggtgaaa caaagccagg
     1741 gccaacagat tgcggcaggc gcctatccga atccctccgt ctccggcagc gccgggcgtg
     1801 gcgccattcg cgacccgagc accggcacgc gtgtgaccga gcgtacgttt acggtggaac
     1861 agccgcttga atggaccggc aagcggcagg cccgccagga agcggccgat gccggagcag
     1921 ccggggcgac tgcggcattc gaggacacgc gcctcaccct gctcgccgac gtgaaagtgg
     1981 ccttctacca actgctcttc gcccagcgcg atgtggacct cgccgcgcaa aacgtcacca
     2041 gcgtggagga agtcttgcgg accgtacacg cgcgcgtggc ggccggcgaa gccacctcct
     2101 tcgacagtat gaaagccggg gtggaagtgc agaaggccaa gaaggaagtc gcccgggcga
     2161 acagtaccct gttggtggcc aaggcccgct tgaataccct caccgccggg tcgctcggaa
     2221 aagagttttc catccagggc gattttcaat cgcccaagcc cggggtgaat gcggaggccc
     2281 tggcgacgca agccatggaa caacatccgg cggtccgccg cctgagcaag ctggcggaac
     2341 aggcggaaca tacgctgcgg tatgagcggg aagcccgcgt gcccaatatc agcctgctgg
     2401 gcagttatca ccgggaggcg ggtgacgaat cggtcaccgc cggactcagt atgcccctcc
     2461 ccctctggta ccggcggcag ggagaaattc aatccgcgct cggcgccaaa caccgcgccg
     2521 atgcggaacg cctgcggcaa cagaacgagc tggagcaggc catcactcaa catgcgcagg
     2581 aagtccgcac ggcgcaggat cagctgcagg tatttgaaac cggcttgttg aaacaggccg
     2641 aacagacctt gaccgtcgcc cgcacgagtt ttcggcacgg cgccgccagt ttgttggatg
     2701 tattagatgc tcaacgcgtg taccggcaaa cccaactgga atatgcgcag gtgcgggccg
     2761 atctctccat tgcgctggcc cggttggagc gggcagtggg agcctcgcta tgagcctcct
     2821 cgcctggttc gcacctcggc aagagacgat gatcggacgc tgcgcaacga ccggaatgat
     2881 cctgaccatc ctctctgttc tccccggatg cggggagcag ggagcggaac cgccgaaacc
     2941 gccctcgccc gtcgaggtgg cgccgcaagc cgccttcacc catgcgattc acccgccgcc
     3001 ggctgtccgg gaacggttgc gcaccgagtc cgccacgcga cgcgccgttc ccgaattggt
     3061 gaccgcaccc ggcgaggtcg cactcgattt gaagaaggtc gccaagatta cctcccgcat
     3121 cggcgggcag gtcgaacata ttcacgtgca gttgggtgac cgggtcaagc ccgctcaacc
     3181 gctcgccgcc atcggcagcc tgcagctcga tcagctcgtg gaagaatacc tcgtcgggaa
     3241 ggcgcaggcc gacgtggcgg aaaacagtct ccgacggacc gagaagctgc gcacggacga
     3301 catcgtgccg gagcgaaagc tcattgaaga caaggggcac tacctggaga cgaaggcacg
     3361 ctatcagcac gtccgcgaaa aactgctggc catgggcatc tcgaaagccg aactcgccgg
     3421 cctcgaacgg ggcggccatg aagaaggcca ccactatacg ctgacgtctc ccatcgccgg
     3481 tacggtggtg gcacagaatg cggtgcgcgg acagggggtc acgcccgggg cagaactctt
     3541 cgaagtcgtc gataccagcc gtgtctgggt ctttgccaac ctgcccatcg aacaggctag
     3601 aaaattcaaa gagggcgacc tgggcacgat cacgccgaaa ggcggcgagg tgatcacggc
     3661 cccactgaca tatctggcgc cggtcgcaga tgagaccacg cgcacgatcc aggttcgctt
     3721 cgaggtggag aaccggcagg gacaactgaa accgcatgaa tatgtggagg cggcgctcac
     3781 cctctcagga gcacccacgg tggcggttcc tgtgtctgct ctcaccacca tcgacaagac
     3841 tcgaggggtc ttcctcgaag gtcccgacgg gtacacgttt gcgccgatcg acgcaggacg
     3901 tgagagcggc ggctgggtcg aaattcgaac gggtgtgaag gaaggcaacc cggtcgtgat
     3961 tgaaggggtt ttcgatctga aaaacgtgct gctgaaagaa cagatcggat cgggcgagtg
     4021 aggaccccat gaaccccctg ctgacatttt cgctccgcta tcgattcttt actctggtgg
     4081 cgatcgcagt cgtgattgcc acgggcatct ggtcctttac gaacctcacc atcgacgccg
     4141 tccccgacct cacaccggtg caggtccaag tgctcacccg cgctccggcc ttgggcccgg
     4201 tggaagtcga gcagtttgtg acgttcccca tcgaggcgtc gttgaacggc ttgccggcat
     4261 tgcgggaact gcgctccgtt tcacgttatg gcctctctgc cgtgacggcg atcttcgacg
     4321 accagaccga catctaccgg gccagacagt tcgtgaccga acggttggcg caggccatgg
     4381 aacgcatccc tgccgagtat ggacgaccgg tcatgggccc cttaaccacc gggctgggcg
     4441 aagtgtatca gttcacggtc aaagggcctg gctacagtcc catggccctg cgaacgctgt
     4501 tgcagtggga catcgggatg aaattacgcg ctgtgcccgg cgtggtcgag gtcaatatct
     4561 ggggaggcga gccacaacag ttccacgtca tcgtggatcc gtcgaaactt ctttccttca
     4621 agttgaccat gaagcagatc ttcgacgcgc tgcaacggaa caatgccatc gccggcggcg
     4681 gatacatcga acatcagcgt gaacaactgc tcattcgagg ggaagcgctc gccacacagg
     4741 tgagcgacct ggcccggatc gtcgtggccc acggatcggg cggtgtgccc gtctacattg
     4801 ccgacgtggc cgaggtaaaa gaaggctcag gactccggat cggcgcagct acggccatgg
     4861 gcgaaggcga gacggtcatc ggaatggtac agatgctcgc gggtgaaaac gcgcaacagg
     4921 tcgtaacccg cgtgaaggcg cgcgtgcagg aaatccaggc gaccctgccc tccggggtca
     4981 ccatcgagcc ctactacgat cgcacaattt ttgtgtccaa ggtcatgacg accgtgcgca
     5041 acaatctgct tgaaggtggc ctgctcgtca tcgccgtgct gtttctcttt ctcggcgacc
     5101 tgcgggccgg actgatcgtc gcctcggcca ttcctctcgc catgctgatc gccttcaccg
     5161 gcatgatgca ggccgggctt tcgggtaatc tgatgagcct gggagccatc gacttcgggt
     5221 tattggtcga cgggtccgtg gtcatgatcg acaacatcct gcgccgatta gcggacaagc
     5281 cggtgcgtaa tcaagaagag cgactcgtga ccgtgctggc ggccgggcgg gaagtcctgc
     5341 gtcccatgac cctggccgtg agtatcatca ttctcgtgta tgtgcccatc ctggccttga
     5401 ccgggatcga aggcaagatg ttccaaccaa tggcgctcac cgtgatcatg gccctggccg
     5461 gctccctgct cctcgcggtc accgtgacgc cgctgctgtc gttctggttc gtacggacgg
     5521 gagtgaaccg gcaagaaact ctggtcattt gcggcctgag agacgcctat gcccctttgc
     5581 tcaagtgggc gatgaggcgg cctgtctggc cggtctcgat tgccatcatc ttgtttatca
     5641 tcagcctggg ggcaggaacc aggctcggca tcgagttcgt gcccaggctg gaagaaggcg
     5701 accttgcgct gcaagtgtgg cggttgccca gcatctcgct gaccgaatcg gtggccactg
     5761 cactcgagat cgagcgggtg ctccgccgtt ttcccgaggt cactcaggtg gtcacgcgca
     5821 cgggcagccc cgaagtggca acggatgtga tgggcgtcga actttccgat gtcttcatca
     5881 tcctcaagcc tcaacaggaa tgggtgacag ccgggactcg cgacggcttg atcgaagcca
     5941 tgaaacgacg catcgaggaa caggtacccg gtgtcggcct cggattcacc caaccgatcg
     6001 aaatgcgttt caatgagttg atcgccggcg tgcgatcgga tctggccgtc aagattttcg
     6061 gccccgacct ggacattctc aagcagcaag ctgaacgcgc ggcgcgcgcg ctccaagccg
     6121 taccgggcgc ggcagacgtc aaggtcgaac aggtcgcagg gctcccgctg ctgcgtgtca
     6181 tcgtggatcg cgcagcaatc gcccgatacg ggctcaccgc cgacgacgta ctcacgctca
     6241 ttcaatcgac cagggtgggg acggtcgtcg ggactgtcgt gcagggctcc cgtcgattcg
     6301 agttggtcgt gcggttggcc gaccgggtct cgcaggatcc ggcgtcgttg ggccgcctgc
     6361 tgatcccgac catgcatggc gaactggtgc ccctctcgcg tgtgacgagc atcacagtgg
     6421 attcaggccc ggcgcaggtc agccgtgaac atgtgcaacg gcggatcgtc gtggagtgca
     6481 acattagagg acgggatcta ggtgggtttg tggctgaggc ccgacaggcc gtcgcccggg
     6541 cagtcaccct gcccgccgga tacgagatgc actggggcgg gcaattcgaa cacctgcagg
     6601 aggccgctcg ccgtctgacc ctggtggtgc cggtcacgct gctgctgatc ctcgggatgt
     6661 tgtcggtgat cttcggcgca atgcggcctg ccctgctgat ctttctcaat gtgccgctgg
     6721 cgctgtcagg cggcattctg gcgctatggc tccgggggtt gccgctcagc atctcggcca
     6781 tcatcgggtt tatcgcgctc ttcggaattg ccgtgctcaa cgcagtcgtc ctggtgagcc
     6841 atatccgccg gctggaagcg gagggcgcat cgaccggcga ggcggttgtc caaggggcca
     6901 tggatcgact ccgcccggta ctgatgacgg ccctggtggc gagcctggga tttctcccca
     6961 tggccttggc gaccagcatg ggcgcggaag tgcaacgccc actggcgaca gtagtgatcg
     7021 gaggcctcct gacctcgaca gccttgacct tgttggtcat tccggccctg tatccctact
     7081 gggtgcgtga cgagaccatg aggccgcggc cccttaccga ccaggcaggc atgaacgaaa
     7141 ccggcgcgta acctattgtc tgaccaaggg gaagcaggag agggtcgaat cgatgcccag
     7201 ctgtttcaga ctctttcctg gacagacttt cgtcaccgcg gtaagcttcc tctacgagtc
     7261 gaattgtctg caaccgagcc cgaatactgc gaaatcgttc catcacacgc agaaccggtg
     7321 cccatcctga ggagtagtta tggcgaccat catggtgatc gacgacgaac catccatccg
     7381 cggcctcctg cgggaggtcc tggaacgatc agggcacaag gtcgtcgagg cgaaagatgg
     7441 gcgggaggcg ctggatctct atcaaaaaca taaagccgac ctg
//
LOCUS       DAORRQ010000117         7475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_390_length_7475_cov_111.290027, whole genome shotgun sequence.
ACCESSION   DAORRQ010000117 DAORRQ010000000
VERSION     DAORRQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_390_length_7475_cov_111.290027"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(488..655)
                     /locus_tag="PK224_19815"
     CDS             complement(488..655)
                     /locus_tag="PK224_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18051.1"
                     /translation="MPIYFLIGLTILILVIAVYATVAEEEDESTTVDSIPPSDWDAQT
                     HEQQHEYRKAS"
     gene            1039..2085
                     /locus_tag="PK224_19820"
     CDS             1039..2085
                     /locus_tag="PK224_19820"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRB18052.1"
                     /translation="MTGNASSPTVLTPREITLHLLGLVLFLGLLGLWYWLSTGARAVP
                     TWIEMLQDDDPSTRIVAAEQLGQVGPAAKAALPQLLAQATGDGSQHANTTAATALKSI
                     DLTAARQVMTHFIPRLQDQDAQQRRTACAVLGSLGPVAKPAVPALLPLAHDSDELVRR
                     NALMALSSIGIPSPPIVAALLEGLRDSSALVRQTAATQFAFTVPLTQPVRTALTTLLN
                     DPDRSMATLARSALDKARPDDAAQLESLGMMVQHSGARDYALHQLAQLGPAAGDVLLP
                     VLPLLQDDRPFVRYLAAEAIGAMGPTAKQALQALQRRREDSDPVVREAVADAIAGIEA
                     AMAPAASSEKATRP"
     gene            2082..2582
                     /locus_tag="PK224_19825"
     CDS             2082..2582
                     /locus_tag="PK224_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18053.1"
                     /translation="MIATTHETSSRLRLLTRLGFWGSVIGGLLFPWAIMLAVEVVVHQ
                     VPVARAWRSFTLHLFAPGYNFFLIGLLTAVPFVMLAVLMLLHLGAAPAQEPLIARRRT
                     LGLAGAGLGMLVLAGWTHLEVLLHPDAQGALAYLYLPVILLASMPIGYGLGRVIARML
                     LPRPSA"
     gene            2658..3635
                     /locus_tag="PK224_19830"
     CDS             2658..3635
                     /locus_tag="PK224_19830"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HRB18054.1"
                     /translation="MIRLLCSLVLLILWHSAVLAASKYEDSVKELAEGVAAEAIKMKK
                     SRLALLDFVDSTGEVTPIGQFLAEELATHLLVAGELKVVDRKLLVATMEKHQLTHLDT
                     SQAKAAKKVAKALRADLYVTGAYLEIPEGVQVTAKLIGPYTVFPVGAARAIIPKAGPL
                     AALLKQANAPKPPAPATPPEPTTPVLNAHENELYKVSVMTISRQDDQIEIDLLFVNRS
                     THPVKIGCQLLDTYLVDEQGEQWRQDVAQNREGLCTRGMELNPRRQQHASLSFIATNK
                     PPTGPVTLHYHETSPRPDRVVTLDGLKLEAAPAAEAEAPAPQAADTPTP"
     gene            3672..4466
                     /gene="thiD"
                     /locus_tag="PK224_19835"
     CDS             3672..4466
                     /gene="thiD"
                     /locus_tag="PK224_19835"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRB18055.1"
                     /translation="MIKQVLTIAGSDSGGGAGIQADLKAMSANGVYAMSVITAITAQN
                     TEEVADVFELPTSIIAAQLDAIFDDFDVAAVKTGMLSSTDIVKTVVKLLKPQQVKNLV
                     VDPVMIAKGGQALLKPDAIDTIKKELFPLALLVTPNVHEAQQLSGIEITSLADARRAA
                     KIIHQFGCANVLIKGGHLASDRATDLLYDGRFFNIYKGEFIDTPHTHGTGCTFASAIA
                     AHIARGKTVPDAVQTAKTYLTEAIRHSLAIGHGKGPTNHFYFLNQG"
     gene            4471..5763
                     /locus_tag="PK224_19840"
     CDS             4471..5763
                     /locus_tag="PK224_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18056.1"
                     /translation="MSRGPGSPSSFLLTGWSWLLLTSLLGLATFLGIVRGSPLPPGLR
                     LLHVHGALIGGLLQIMTGLALTAVALAGHDTKGQKHRLLFVSMNLSALGLAAGAWLRD
                     STLTIGAGLLLAALFVPMVRHLMLGLRTSSGWTPLSTFYFGISLTGLFGTLALGEFLA
                     GAWFPTWHGILRLGHLHAGLLLFLTLGVVGAMQTTMPGLMNRPLRSAVLGQIVLLLLP
                     ACAAGLITGFLLSSVHIQLVAGGGFLVTLALGSVNLLRTWNQAGQPGSAATDHLMIAL
                     CLLLIMTCIGMAVGINVLWTPPVMPYGTLHLVAYTHTAFLGFFLQATVAGLSYALPAL
                     LAAQRVTSHKKRVAYQDTLAQIANRWRALQVSTLSFGTLGLVLVASLTWNLPLSSIWI
                     QAGTWGSLGLLLLHLTMVTMKITQMVGTMPTGDRAATH"
     gene            5784..6602
                     /locus_tag="PK224_19845"
     CDS             5784..6602
                     /locus_tag="PK224_19845"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRB18057.1"
                     /translation="MPTIQPREETVFFHDRLGHRIAAVLARPEQTTDHVAVLCHGFLS
                     HKNSSSNQALTELLIGRGIATFRFDCFGHGDSDGPFAKLTTTIGVGQALAALHYLLTR
                     GYHRLALVGSSFGGLLSILAAADWTRMHTSKPASIPPLACLALKCPVVDFGEELRLEL
                     GEDGLQEWKQTDTIPDLHGGATRLPLDYVFYQDCLNRIAYEPARTIAVPTLIVQGDHD
                     EYVPLHQSQRLFEALPGPKRLEILPGADHRFTKASDFQRMLTLLTEWVTRHPRT"
     gene            6874..>7475
                     /locus_tag="PK224_19850"
     CDS             6874..>7475
                     /locus_tag="PK224_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18058.1"
                     /translation="MKTSTSVLVMGAVGCLSSALLQTGGASYAVAADSATTMSLLQQP
                     SLAAAVTKAQGSGTAPILPNPTALVLTAQKGNTAVGTLTLKKSSTDQHTYYLSTNQSW
                     IWMNPPYGSTQSITSETDQLVITAQTANLPAGTHSAVVYVVDSGPNNFTNMLRIPVTF
                     TVTASPVATPPPPPPATPPAVTPPGKKLLMTEVALRDGHQ"
BASE COUNT         1447 a         2466 c         2074 g         1488 t
ORIGIN      
        1 cgatctcatc acattcctcc ttatacaggt gtctgtgaaa tcgggggaag gtcaatatac
       61 tcacgcaaat ccttatctcg gaccaagcgc ttcttgatgg attgcaggat ttaaagactc
      121 acgggatcga ttataggtta cttcttcctg atttagacgg cgctgctcgc cacgcgaatg
      181 caatatggga cttcggttaa gacaattgaa atcctgttaa tctcccgaaa cagataaaac
      241 aaagggggtg ggagtctttt atcagaccga ctatcacagg ctgatggtta ggccaccctc
      301 gactcggagt gtggtttctg aggaaggcgc cgctcgaatt ggtgattcgc tgagggccat
      361 cgcggcaggt ctgcacaatc atccgaaacc gtcagcgtgg aacacaccgg gtggcagttc
      421 atcgccctcc tgctccatgc tcgcgtgaag gaagtggccg ggtgatcgaa gcgctgtgta
      481 tccccgctca actcgctttg cgatattcgt gctgctgttc gtgcgtctgc gcgtcccaat
      541 ctgagggcgg aattgagtcc accgtcgtgg attcgtcctc ttcttctgca accgtggcat
      601 agactgcaat cacgagaata aggatcgtga ggccgataag gaagtagatg ggcatggtga
      661 cctcctgtgt tactggtggt cctactgtca tgctagccct gccatcggcc atcgactacg
      721 agggaaatcc ccaggttttg cccggaatat ccccgtagcg ggaacatgga cagcgatccg
      781 ccccgatgta agactctgcg caattccatc cagcctcaag cgctgcctcc cttgtccgcc
      841 aagagactcc tgcactctat gaaccagcaa ttccggtcta acccgttcct gctaatctct
      901 ttcacctcac taacaaccgg tatcaaagtc acctttccgg ggagggccac acactgctgc
      961 ctactcgcaa ctcagcttcc ccctctgttg tgataggctg atctcggcac cttgcacttc
     1021 acgatattgg ccgtccgcat gaccggcaac gcttcgtcgc ccaccgtcct gaccccgcgc
     1081 gagattacgc tgcacctgct tgggctcgtt ctcttcctcg gcctgctggg tctgtggtat
     1141 tggctttcca ccggcgcacg agcggtgccg acatggatcg agatgctcca ggacgacgat
     1201 ccatccacac gcatcgtcgc agcggagcag cttgggcagg tcgggccagc cgcgaaggcg
     1261 gcacttcctc aattgctggc gcaagccacg ggggacggca gtcagcacgc gaacaccacc
     1321 gcggctacgg ccctgaaatc gatcgatctg acggcggccc ggcaggtcat gacccacttc
     1381 attccgcgcc tacaggacca ggatgcgcaa caacgccgca ccgcctgcgc agtactcggg
     1441 agcctgggcc cggtggccaa gccggcagtg ccggccctgc tgccgttggc gcatgattcc
     1501 gatgaactcg ttcgccgcaa tgcgctgatg gccctgtcaa gcatcggcat tccgtcccca
     1561 ccgatcgtag ccgccttgct ggaaggcctc cgcgattctt cagcactggt ccgccagacc
     1621 gccgccaccc aattcgcctt tacggttccc ctgacacagc ctgtgcggac tgcgttgacc
     1681 acgctgctga atgatccgga tagatccatg gccacgctgg ctcgcagtgc gctggacaaa
     1741 gcaaggccgg acgatgcggc acaactcgaa tccttgggca tgatggtcca gcattcaggc
     1801 gcgcgcgact atgccttgca tcagctggcc cagctcggtc cggcggcagg cgatgtccta
     1861 ctcccggtcc tgccgctgct ccaggatgac cggccgttcg ttcggtacct cgcagcagaa
     1921 gcaatcggcg cgatggggcc caccgcgaag caagcgctcc aggcccttca gcggcggcga
     1981 gaagactccg atcccgtcgt acgggaagcc gttgccgacg ccatcgccgg gattgaggcc
     2041 gcaatggcac cggcggcatc ctccgaaaag gcgactcgtc catgatcgcg acgacacacg
     2101 aaacctcctc gagacttcgc ctcctgactc gcctgggttt ctggggaagt gtgatcggcg
     2161 ggctgctgtt cccctgggcc atcatgctgg cggtggaggt cgtagtgcac caggtccccg
     2221 ttgcacgggc ctggcgatcg tttaccctgc acctcttcgc tcccggctat aatttcttcc
     2281 ttatcggcct gttgacggcc gttcccttcg tgatgctcgc ggtgctgatg ctgctgcacc
     2341 tgggagccgc tcctgctcag gagccgctga ttgcgcgtcg acggacattg gggctcgccg
     2401 gcgcaggact cggcatgctg gtccttgcgg gctggaccca tcttgaggtg ttgctccatc
     2461 cggatgcgca gggggcgctt gcctatctct atctgccggt catcctgctg gccagtatgc
     2521 cgatcgggta cgggttggga cgcgtcatcg ccagaatgct cctgccgagg ccttccgctt
     2581 gaatgacacg ggctggacag tatcgacgac gtgagtataa tgtgggccat gcgtgattat
     2641 gccgtgagtg cctcccgatg atccgtctcc tctgctccct cgttctgctc atcctctggc
     2701 actccgccgt cttggctgcg tcgaagtatg aagacagcgt gaaggaattg gccgaagggg
     2761 tggcggccga agccattaaa atgaaaaagt cccggctcgc gctgttggat ttcgtggaca
     2821 gcacgggcga ggtgacgccc atcggtcaat tcctcgccga ggaactggcg acccatctgc
     2881 tggtggccgg agagctgaaa gtcgtcgatc gcaagctcct ggtcgccacc atggagaaac
     2941 accagctcac ccatctcgac acgtcgcagg ccaaagccgc caagaaggtc gcgaaggcat
     3001 tacgcgccga tctctacgtc accggcgcct atcttgaaat tcccgaaggc gtgcaagtca
     3061 ccgccaaatt gatcggcccc tacaccgtgt ttccggtggg cgccgcccgg gcgatcatac
     3121 ccaaagccgg cccgctcgcc gcgctgctca agcaggcgaa tgcccctaaa ccgcccgcac
     3181 cggcgacccc accggaaccg accaccccgg tcttgaacgc ccatgagaat gagttgtaca
     3241 aagtcagtgt gatgaccatc tccaggcagg atgaccagat cgagatcgac ctgctgttcg
     3301 tcaatcgcag tacccatccg gtcaagatcg ggtgccaact tctggacacg tacctggtgg
     3361 atgagcaggg cgaacagtgg cggcaggatg tcgcccagaa tcgcgaaggc ctctgtactc
     3421 gcggcatgga actgaacccc agacggcagc agcatgcgag cctctccttc atcgcgacga
     3481 acaaaccgcc cacgggaccc gtgacgctgc attaccacga gacctcgccg aggcccgacc
     3541 gtgtcgtcac cctggatgga ctgaaactcg aagccgcgcc ggccgccgag gccgaagcgc
     3601 cggccccgca ggcagcagac acccccacac cataagtgat tatcttcgga atagaggcac
     3661 aggaaacctt catgattaaa caagtcttga cgatagcggg ttcggactcg ggcggcggag
     3721 cagggattca ggcggacctg aaggccatgt cggccaacgg ggtgtatgcg atgtcggtca
     3781 tcaccgccat cacggcccag aacacggaag aggtggccga cgtgtttgag ttgcccacct
     3841 cgatcattgc cgcccaactc gacgcgatct tcgacgattt cgacgtcgcg gcggtcaaaa
     3901 ccggcatgct ctcctccacc gacatcgtca agaccgtcgt gaaactcctc aaaccgcagc
     3961 aggtgaagaa cctggtggta gaccccgtga tgatcgccaa gggcggtcag gccctcctga
     4021 agcccgacgc catcgacacg atcaagaagg aactgtttcc actcgccctg ctggtgacgc
     4081 cgaacgtgca cgaagcccaa cagctctcag gcatcgaaat cacctcgctc gccgatgccc
     4141 gccgggccgc taagatcatt caccagttcg gctgcgccaa cgtcctcatc aaaggaggac
     4201 atttagcgag cgatcgcgcc accgacctgc tgtatgacgg ccgcttcttc aatatctaca
     4261 agggtgagtt catcgatacg ccgcataccc atggcaccgg ctgcacgttt gcgtcagcca
     4321 tcgcggcgca tatcgcccgg gggaaaaccg tccccgatgc cgttcaaacc gccaagacct
     4381 atctcaccga ggccatccga cacagcctgg ccatcggaca cggcaagggt cccaccaacc
     4441 atttctattt cttgaatcag ggttagcgct atgagtcgag ggcccggatc cccatcgtcc
     4501 tttctgctga ccggatggag ctggctgctg ctgacgtcac tgctcggact cgcgaccttt
     4561 ctcggcatcg tgcgcggctc cccgctccct cccgggctgc gcctattgca cgtccacggg
     4621 gcgcttatcg gcggcctcct ccagatcatg accggcctgg cgctcaccgc agtcgccctc
     4681 gccggccacg ataccaaagg gcagaaacac cgcctgctgt tcgtcagtat gaatctctcg
     4741 gccctcgggc tggccgccgg ggcctggttg cgtgactcca ccctgaccat aggggccggg
     4801 ctgttgctgg ccgcgctgtt tgttcccatg gtacgccacc tgatgctggg acttcggacc
     4861 agttccggct ggactccctt gtcgaccttc tactttggaa tcagcctcac cggactgttc
     4921 ggcaccctgg ccctgggcga gttcctggcc ggcgcctggt tccccacctg gcatggcatc
     4981 ctgcgtctgg gccatctgca cgcggggctt ttgctcttcc tcacactggg ggtcgtcgga
     5041 gcgatgcaaa cgacgatgcc cgggctgatg aatcgccccc tgcgcagcgc cgtcctcgga
     5101 cagatcgtct tgctccttct gccggcttgc gccgcgggac tgatcacagg atttttattg
     5161 tcctccgttc acattcaact ggtcgccggc ggtggcttcc tggtgacgct ggcgctcggc
     5221 agcgtcaatc tcctccgcac ctggaaccag gccggccagc cgggatccgc cgccaccgac
     5281 caccttatga tcgccctgtg tctgctgctc atcatgacct gtatcggcat ggcggtcggg
     5341 atcaatgtcc tgtggacccc gccggtgatg ccttacggga cgcttcacct ggtcgcctat
     5401 acccacacgg cgttcctggg atttttccta caagcgaccg tcgccggact gtcttatgcg
     5461 ttgcctgcgc tgctggcagc gcaacgggtc accagtcaca aaaagcgggt cgcctatcag
     5521 gacacccttg cgcaaatcgc gaaccgatgg cgggcgctgc aggtgtccac gctcagcttt
     5581 ggaaccttgg ggcttgtcct cgtcgcctcg ctgacctgga atctcccgct ttcttcgatc
     5641 tggatccagg caggaacctg gggaagtctg ggacttctct tgctccacct gacgatggtg
     5701 acgatgaaga tcacgcagat ggtaggaacg atgccgaccg gcgaccgcgc cgcaacacat
     5761 taaccagact cgaatcaccg gttatgccaa cgattcagcc cagggaagaa acggtctttt
     5821 tccacgacag gctcggccac cgtatcgcag ccgtcctggc caggcctgaa caaacgacgg
     5881 atcacgtcgc ggtcctttgt cacgggtttc tctcccataa aaacagttcg agcaaccagg
     5941 ctctgaccga gctcttaatc ggccggggga ttgccacgtt ccggttcgat tgcttcgggc
     6001 atggcgacag cgatggaccc ttcgccaaat tgacgaccac catcggagtc ggccaggcgc
     6061 tcgccgcctt gcactacctc ctcacacggg ggtaccaccg gctggcactc gtgggctcca
     6121 gcttcggcgg tctcctgtcg attctcgccg ccgctgactg gacaagaatg cacacctcga
     6181 aaccggcatc cattccgcca ctggcctgcc tcgcgctgaa atgccctgtg gtcgatttcg
     6241 gagaagagct tcgcctggag ctgggagaag acggccttca agaatggaaa caaaccgaca
     6301 cgatacccga tcttcacgga ggagccactc gccttcccct cgactatgtt ttctaccagg
     6361 actgcctcaa ccggattgcg tatgaaccgg cccgaaccat cgccgttccc acgttaatcg
     6421 tgcagggtga tcatgatgaa tatgtaccgc tccaccaaag ccagcgactc ttcgaggcct
     6481 taccgggacc taaacggctg gaaattcttc caggcgccga tcaccgcttc acgaaagcct
     6541 ccgactttca gcggatgctg acgctcctca ccgaatgggt cactcgccat cccaggacct
     6601 gataccgtcc ttttcctaca tcgccgaccg acgacagtcc ccgaacctcg ttcacaatcc
     6661 cggttactca tcgatgttgc agaatcgcgt cccgccggag gctttctcct gcagccactg
     6721 ctccatcttg cacagcaaaa aataacgact agaaacccgc agctcgattg cccgcaaaat
     6781 ggctcgtgac ggggctttct ggcgatcgca ccacttcgac gcctggcaca ctggttgctc
     6841 atccagcgtc tcgattttcc cggaggaact acgatgaaga cgtcaacgag cgtgcttgtg
     6901 atgggtgctg tcggttgttt gtcttccgcc ctgctccaga caggaggggc ttcatatgcg
     6961 gtagccgcgg acagcgccac cacgatgagt ctgctgcaac agccatccct tgcggcagct
     7021 gtcacaaaag cccagggcag cggaaccgca cccatcctgc ccaacccgac cgcgctggtc
     7081 ctgacggctc agaaaggcaa cactgccgtc ggtacgctca cgctcaagaa gtccagcacg
     7141 gatcaacaca cctactatct cagcacgaat caatcgtgga tctggatgaa cccgccctac
     7201 ggcagcaccc agtccattac ctccgaaacc gatcaattgg tcatcacggc gcaaaccgcc
     7261 aacctgccgg ctggaacaca ctctgcggtg gtctatgtcg tcgactcggg ccccaataac
     7321 ttcaccaata tgctacggat cccggtcacc ttcaccgtca cggcctcgcc ggtcgcgaca
     7381 ccaccgcccc cgccgcccgc aacgccgccg gcagtgaccc cgccggggaa aaagctgttg
     7441 atgaccgagg ttgcgttgcg cgacgggcat caatg
//
LOCUS       DAORRQ010000118         7439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_392_length_7439_cov_103.959642, whole genome shotgun sequence.
ACCESSION   DAORRQ010000118 DAORRQ010000000
VERSION     DAORRQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_392_length_7439_cov_103.959642"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(698..1591)
                     /locus_tag="PK224_19855"
     CDS             complement(698..1591)
                     /locus_tag="PK224_19855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB18059.1"
                     /translation="MGRAGRIARGIMVLVSVLLVAACGDSFNDAYCEANNHRNQQTVE
                     HLTNVLEQKRNQPGLYLARARCHYFLGAYAQALQDASEVIRRLPNKADAYLLRAKAGK
                     MLGQIPQALQDYSAAIKFQPTAEAHYGRGLTYLREYQGKDREALEDFTAAIRLDPKHV
                     GAHAFRAQIYSRHEQFQHALADSETVLKLDPTTTNAYCNVGFAHYALGHDAEGRKFLT
                     TCYQKDPDPQTRGYYETEVNKVLYARKPKRNPGGSDVAEGGVKTPYDREMERQQNVYE
                     SLRNSGFNDRAEACRTDGAKC"
     gene            1925..2635
                     /locus_tag="PK224_19860"
     CDS             1925..2635
                     /locus_tag="PK224_19860"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRB18060.1"
                     /translation="MNGFDTAVLGFFNQFAQRSWAVDSLIMTISDSDALKGYLSLPLI
                     WWAWFRAPHREDHRQTVLLTILAAFAAMAIARVLQLVLPYRARPVHTAGLDFTLPYTM
                     PADFLAGWSSFPSDHASLFFALAAGLWLVSPLVGAFAFLHAAVIVSIPRIYVGLHFPT
                     DILSGACLGVTAVAVIWKWRAVFVKLVEPALAWSKTSPHLFYPAFFILTSELAHLFKD
                     SRWLAESLWHMAKLSLAR"
     gene            complement(2835..3287)
                     /locus_tag="PK224_19865"
     CDS             complement(2835..3287)
                     /locus_tag="PK224_19865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRB18061.1"
                     /translation="MVAGSTQGFPLERQRIGLDINVFIYFLEDHPRYGAWCASLFDLI
                     ERGLNPAVTSTVTLLELLVQPYRDQKDELAQRIFALAGTYPRLEWVSVTMNVADRAAE
                     LRARYRLSTPDAIQLATAILHKAVRFYGNDRGLRKVKEIECVLIDELI"
     gene            complement(3281..3499)
                     /locus_tag="PK224_19870"
     CDS             complement(3281..3499)
                     /locus_tag="PK224_19870"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRB18062.1"
                     /translation="MAVQKLSSKNQIVLPKEARQAMGVRGGDELLIVVKDDLTLVMPK
                     PKRYAQTLRGLAKGTYPSGYLKRERRSW"
     gene            complement(3890..4675)
                     /locus_tag="PK224_19875"
     CDS             complement(3890..4675)
                     /locus_tag="PK224_19875"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRB18063.1"
                     /translation="MKPRRRRVFASWLGAVLTLMTAPLFALDHDNLDPNRPIGMEDAY
                     AIPKGEIGLEGGVRFNDRREGRTQVTFQPQIIYGAFANTQIEIQGDLFTDPHSIVGAN
                     KSGDLHLGVLYNFNTETLMLPALAVRVEADLPTGVNSKGVDTQLTGILTRSFGRLRVH
                     LNAGYTVIGSPQGQERPGAYRAVAAVSYPLGYPTSFRDTLIVSVYTRQSDLRGQQNHT
                     GIEVGLRHQLTSRVVLDGGLGTEFVGPADRAALLGTMGVSVGF"
     gene            complement(4672..5619)
                     /locus_tag="PK224_19880"
     CDS             complement(4672..5619)
                     /locus_tag="PK224_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18064.1"
                     /translation="MTIGKYMCMLAIVATAMVAFALAGCSQFEPRDKRFYYRALWNFS
                     LREDLAELDSEFNGVDFGHSNLYENLLLTGGQDVPAIEERARKQTIAFIATKPRLNPN
                     EEAIAPTYMKLAWRAQNTFDEAHALHRATYDIAVSDEPEKDRAIRNVLAYYKDSAYAI
                     TSKRLDHHRLDQFPYSKAFRTRFPLFNATIWSYHYLQVAVYDPLQAARDLAAKTQAVR
                     PILATYRRYLEQPPVQWTFMPLTAELSPQFAARYPELADIFDNLHMLHDNISDILTSE
                     RLPTWEAKRAEIYRVLNSYYLASADATNPMIVLGQEHHH"
     gene            complement(6014..6610)
                     /locus_tag="PK224_19885"
     CDS             complement(6014..6610)
                     /locus_tag="PK224_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HRB18065.1"
                     /translation="MAHHQSATITAPRQPAPRSHDHHINGHDCVQAHGDHLGRHQPHE
                     HQEANHQASSLDRTALMATLHCLSGCTIGEVLGMAIGTALGWSNWPTVGLAVALAFLF
                     GYAMTLYPLRRAGMAWGTALGLAFASDTLSMTTMELVDNAIMVMVPGAMDAGLPDPLF
                     WGSLVLSLLLAGVAAFPVNRWLIARGKGHALVHGQHCH"
     gene            7184..>7439
                     /locus_tag="PK224_19890"
     CDS             7184..>7439
                     /locus_tag="PK224_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18066.1"
                     /translation="MPRGILRALCGLVLLFVPLFAQAADTPDTTLSPLKFLVGEWKGA
                     DAEGKAHKIAFALSSGGTSLTETLTPPDSPPMTTMYYSDGD"
BASE COUNT         1407 a         2388 c         2108 g         1536 t
ORIGIN      
        1 cgatctcatc acattcctcc ttatacaggt gtctgtgaaa tcgggggaag gtcagcgctc
       61 aacctagctg aggctcgacg cacaaggaga tctgcgagtc agctccgcac cagtctatct
      121 ccgtggttga gcttgacata gagaaacaag aaatccctgc gtaggcgagc tcgggttgca
      181 gacgcaccat cctattgtgg cttctcacac tcaactcaac cattccacaa catgtgcggg
      241 acagatggga cggagtgtga tcgctcggtg cagacagtgc gaccgggtgt gaactggtct
      301 ggtgacgagt ttcgaaacag aaaaggatgc gtccgaattc gagaaggctc tggccggcat
      361 acgatgcggt caccctgctc tgcctaattc tccctccact tacttcttac ttccctcccc
      421 cctcaagtag tggcgtgact ccgttcccaa ctgcgcgcgt cgaacgaggc ccttccaagg
      481 gcgcgccgaa atctaatgct ccctccactt tctcgctcta ctccccaagg ggtggcctgg
      541 cgggtctccc agtgcgcgtc gtccaacgag ggctttcgga agccgcgcgt tgcgcgaggt
      601 ccacgacgcg acgaataagg agcgtcacgt ttgtggacgc cggcgaggcg gtgagccggc
      661 agtggttctt gacccgtcag gccaccccgc ccctctctca acatttcgcc ccatccgtcc
      721 gacaagcctc ggcccgatca ttgaagcctg aattccgcag gctctcgtac acattctgct
      781 ggcgttccat ttcccgatcg tagggcgtct tgaccccgcc ttcagcgacg tcactcccgc
      841 cgggattccg cttgggcttc cgcgcgtaca acactttatt cacttccgtt tcgtaatagc
      901 cgcgcgtctg ggggtcggga tctttctggt aacaggtggt gagaaacttg cggccctcgg
      961 cgtcatgccc cagcgcatag tgcgcaaagc cgacattgca gtaggcgttg gtggtggtgg
     1021 gatcgagctt gagcactgtc tccgaatccg ccagtgcgtg ctgaaactgt tcgtggcggc
     1081 tgtagatctg agcgcggaat gcatgggcgc cgacatgttt gggatccagg cgaatggcgg
     1141 cggtgaaatc ctccagggct tcgcgatctt ttccttggta ttcccggagg tacgtcaacc
     1201 cgcggccata gtgggcttcg gcggtcggct ggaatttaat ggcagcgctg tagtcttgca
     1261 gcgcctgcgg aatctggccg agcatcttcc ctgccttggc gcgaaggagg taggcatcgg
     1321 ctttattggg cagacggcga atgacctccg acgcgtcctg cagggcttgc gcataggcgc
     1381 cgagaaaata gtggcagcgg gcacgcgcga gatagagtcc gggctgattg cgcttttgct
     1441 cgaggacgtt ggtgaggtgt tcgacggttt gctgattgcg gtggttgttc gcttcgcaat
     1501 acgcgtcatt gaatgaatcg ccgcaagcgg cgaccaacag gacactgaca agtaccatga
     1561 ttccgcgtgc gatccggccg gcccgtccca tgcctgcctc ctggcgacgg gcgcattatc
     1621 tgcctagggc cgaatggccg tcaagaaggt gctgatcgac ctactgcaac agggagatcc
     1681 attccatact ggacgtcttc gaccagggca catcggccct ggccggcgcc ggacacgagc
     1741 ctcacgtcga gcgctcgaat gacgaaaaca tttttcgatt ttctaccggt ctggcttata
     1801 agcatttaca acagccctga cctccgctat cttgccgttg ccgcacacct gcgctccggc
     1861 atgagccgag ccccatgacc actgattcac gtgatacgaa ctccactctg cagatgacgc
     1921 gcacatgaac ggattcgaca ccgccgtctt aggtttcttc aaccagtttg ctcaacggtc
     1981 ctgggccgtg gacagcctga tcatgacgat cagcgacagc gatgcgttga aggggtatct
     2041 ctctctgccg ctcatctggt gggcctggtt tcgcgctccc catcgtgagg accatcgaca
     2101 gaccgtcctc ctgacgatcc tcgcggcctt cgcggcgatg gcgatcgccc gggtacttca
     2161 gctcgtcctc ccctaccgcg cacgtccggt gcacaccgcc ggattggatt tcaccctgcc
     2221 ctacaccatg ccggccgatt tcctggccgg gtggagttcc tttcccagcg accatgcctc
     2281 actcttcttt gcccttgccg ccggcctctg gttggtgtcc ccgctggtgg gcgccttcgc
     2341 ctttctccac gcagcggtga ttgtatccat cccaagaatt tatgtcggtc tccactttcc
     2401 gacggacatt ctgtccggcg cgtgcctcgg tgtgacggcc gtggccgtga tctggaaatg
     2461 gcgtgctgtc tttgtgaagc tcgtggaacc ggccctggcc tggagtaaaa ccagcccgca
     2521 cctgttttac cccgctttct tcatcctcac gtccgaactg gcccatctgt tcaaagacag
     2581 ccgctggttg gcggagagtc tctggcacat ggcgaaactg agcctggcac ggtagcaccg
     2641 gtcgaaaggc ggtcttcgcg ccacaacctt ggacagcatg cctcagacct ttttcctacc
     2701 gcgagaggct tgcggaagca catatcggag cggcttccga ttgccgatag ggcagcctca
     2761 cactacgttg aaaatcgcag ggccagatgg aaaggcttgg gcggaacggg gcaacggggt
     2821 aggagtctac gaagtcagat caactcatct ataaggacac actcaatctc tttcaccttt
     2881 cgcaaaccgc gatcattccc gtagaagcgc acggccttgt gcaagatggc cgtggccagc
     2941 tgtatcgcat ccggtgtgct gagacgatac cgggcacgga gttcggctgc acggtccgcc
     3001 acattcattg tgaccgaaac ccattccagc cttggatacg tgccggcaag agcaaaaatg
     3061 cgttgagcca actcatcttt ctggtcacgg tacggctgga ccagcaactc caggagggtc
     3121 acggttgatg tgacagctgg attgagccca cgctcgatca aatcgaacaa cgaggcacac
     3181 catgccccat acctaggatg gtcttccaag aaataaatga atacgttgat atcgagtccg
     3241 atgcgttggc gttcgagggg gaacccttgt gtggagcccg ctaccacgac cgtcgctctc
     3301 gcttgagata gcctgacgga taggtgccct tagcaagccc tcgcagagtc tgcgcatacc
     3361 gtttgggttt cggcatgacc aacgtcagat catccttgac gacgatcagc aattcatcac
     3421 cgcctcggac gcccatcgcc tgcctcgcct ccttcgggag gacgatctga tttttgctgc
     3481 tgagtttttg gaccgccatg gtgcctccct ccgttgcttc ctacattctg ctttacgtta
     3541 gcataaggaa gtaaagcgct caagagtaag catccaccga caaataggtc gcgagtagca
     3601 gaggaccttg ctttcgcgcg ttgcacgcac agcatcccga acactttctc caaacctgcc
     3661 gtataactct aaccaccagc aactatgctc atgctggttc ccctgcgctc ctcgagcgac
     3721 gccattctga gggcgcgcgt tccgccaggt gtgcgatgcg aggaagggca cgccgcctcc
     3781 cccccccccc ccccaccccc gccccccccc cccccacccc cccccccccc cccccgcccc
     3841 cccccccccc cccccccgcc cccccccccc cccccccccc cccccccccc taaaatccga
     3901 cggagacccc catcgtcccc agcagcgccg cacgatccgc cgggccgacg aactcggtgc
     3961 cgagcccgcc gtcgagtacg acacgcgagg tcaattggtg ccggagtccc acttcgatgc
     4021 cggtgtggtt ctgttgcccg cgcaggtccg attggcgggt gtagacactg acaatcagcg
     4081 tgtcgcgaaa gctcgtgggg tagcccaaag ggtaactgac ggcagcgacg gcccgatagg
     4141 cgccgggccg ctcctgtcct tgcggcgacc cgatcacggt atagcccgca ttgaggtgca
     4201 ctcgcagccg gccgaacgac cgggtcaaga tgccggtgag ctgcgtgtcc acacctttgg
     4261 agttcacgcc ggtgggtaga tccgcctcga cccggacggc caacgccggc agcatcaacg
     4321 tttccgtatt gaagttgtag aggaccccca ggtgcaaatc gccggacttg ttcgccccca
     4381 cgatggagtg cgggtcggta aacagatcgc cctggatttc gatctgcgta ttggcgaagg
     4441 cgccgtagat gatttgcggt tgaaacgtca cctgcgtgcg gccttctcga cggtcgttga
     4501 acctcacccc gccctccagg ccgatctccc ctttcggaat ggcataggca tcctccatcc
     4561 cgatcggacg gttgggatcg aggttgtcat ggtccaaggc gaacagcggc gcggtcatca
     4621 gcgtgaggac cgcgccgagc caggacgcaa agactcgcct ccttctcggc ttcaatggtg
     4681 atgctcctgt cctagcacga tcattggatt ggtcgcatcc gcactcgcca gatagtagct
     4741 attcagcaca cgataaattt cagcccgctt cgcctcccag gtcgggagcc gctcgctggt
     4801 gaggatatcg ctgatgttgt cgtgcagcat atggagattg tcgaagatgt cggccaattc
     4861 gggataccgc gcggcaaact gcggactcag ttccgccgtg agcggcatga acgtccactg
     4921 caccggtggc tgttcgagat accgccgata ggtcgcaaga atgggccgga ctgcttgcgt
     4981 cttggcggcc aggtcacgcg cggcctgcaa cgggtcgtac acggccacct gcaagtagtg
     5041 gtacgaccag atcgtcgcgt tgaacagcgg gaagcgtgtc ctgaaggcct tggaataggg
     5101 aaactggtcc agccgatggt gatccaatcg cttcgaggtg atcgcgtagg cgctgtcctt
     5161 ataataggcc aacacattgc ggatcgcccg atccttctcc ggctcgtccg acaccgcaat
     5221 gtcgtacgtg gcgcggtgca gggcatgggc ttcgtcgaac gtgttctgcg cgcgccaggc
     5281 cagcttcata taggtcggcg caatcgcctc ctcgttcggg ttcagccggg gtttggtcgc
     5341 gataaacgcg atcgtctgct tgcgcgcccg ctcctcaatg gccggcacgt cctgaccgcc
     5401 ggtcagcaac agattctcgt acagattgga atggccgaag tccacgccgt tgaactcact
     5461 gtcgagttcg gccagatctt cccgcaacga aaaattccac agcgcccggt aatagaagcg
     5521 cttgtcgcgc ggctcgaact ggctgcaccc cgccagcgcg aaggcaacca tggccgtggc
     5581 cacgatcgcc agcatgcaca tatatttccc gatcgtcatg ctgaatcgct cctcaatcaa
     5641 actgatcaaa accctggccc ggactattca tgaatacctg ctgcgtcgtt cgattctctc
     5701 gcccggcggg gttgttctcg ttcggcctcc tcgacggact gcaagtacgc ctgcgtcggc
     5761 ctggcttgcg agaagccccg gcgggcttcg gtctcgaacg cctcgcgacg acattcatga
     5821 ataatccggg ctagcgccgg ttcgttgcga catcgtcaag ccgcgtcgcg agacgggcgc
     5881 gcggagtcgt gagggactgc ggcgtactgt gaagtacgtc gaaggactga acgacgagcc
     5941 cgcccgcctg gctgcccctc atcagcagcc aggcatgtcg cagcagcgga tcgacggttg
     6001 cagccgaacc gcctcagtgg cagtgctgcc catgcaccag cgcatgcccc ttaccccgcg
     6061 cgatcaacca gcggttgacg ggaaatgccg ccactccggc cagaagaagg gacaagacca
     6121 gactgcccca gaacagcgga tcaggcaacc cggcgtccat cgcaccggga accatcacca
     6181 tgatcgcatt gtccaccagt tccatagtcg tcattgagag ggtatcggac gcaaaggcca
     6241 accccagcgc cgttccccag gccatacccg cccgccgcaa cgggtacaag gtcatggcat
     6301 acccgaagag aaaggccagc gcaacagcga ggccgaccgt cggccaattg ctccagccca
     6361 acgccgtgcc gatcgccatc ccgagcactt cgccgatggt gcagccgctc aagcagtgca
     6421 gggtcgccat cagcgctgtt cgatcgagcg aggatgcctg gtggttcgct tcctgatgct
     6481 catgaggctg atgacggccc aggtgatcgc cgtgagcctg cacacagtca tgcccgttga
     6541 tgtggtgatc gtgacttcgc ggcgctggtt gacgcggtgc ggtgatcgtc gctgattggt
     6601 gatgagccat gtacgtcctc cctgtgagtg gtgaaatcag ccgttcaccc ctacagacgg
     6661 aacaaggcgg gaattattac agggcgccga ccgttctttt cacatctacc ggtaggcgga
     6721 ggaactactc gcacgtgcag ttgaaacagc cttgtctggt gcaaagcccg caagtctgtt
     6781 cgaggctggc ccgcagggcc tggcgcgcac gatggagacg cacggtgagg ttattcggcg
     6841 tgatttgcag cgcccgctcc acggccgcag ctgactcgcc ttcgagatcg atgcgctgga
     6901 gttattcggc ataggccggc ctgccgttcg tgtctcagga aaactatcgc cagacctgtt
     6961 ctatcaaatt catggcggac gctcccactc ccccggcaat tctcgcccga acgcgcctcc
     7021 tgtctttccc ctcactccaa gtcattatac gtgcatttct gattcaccct gccgttcctg
     7081 ctgtcccgtt ctgcgacagc ggctgaaaaa gtcttgatct cgaccttgga gacatgcttg
     7141 actgacttgt gttcggcatc ttcttgtttg atggagggtt tgcatgccgc gtggaattct
     7201 tcgtgctctc tgtggtctcg tcctactctt cgtcccgctc ttcgcccaag ccgccgatac
     7261 tccagataca accctgagtc ccttaaagtt cctcgtcgga gaatggaagg gggccgacgc
     7321 ggaagggaaa gcccacaaga tcgcctttgc gctcagttcg ggaggcacca gtctgaccga
     7381 aacgctcacc ccgcccgaca gccccccgat gacgaccatg tactacagcg acggcgacc
//
LOCUS       DAORRQ010000119         7436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_393_length_7436_cov_548.939439, whole genome shotgun sequence.
ACCESSION   DAORRQ010000119 DAORRQ010000000
VERSION     DAORRQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_393_length_7436_cov_548.939439"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..7401)
                     /locus_tag="PK224_19895"
     CDS             complement(157..7401)
                     /locus_tag="PK224_19895"
                     /inference="COORDINATES: protein motif:HMM:NF018319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRB18067.1"
                     /translation="MIGQLQLGNNNITGDDPTALVLPGKTRMTRLQAEQFVQRYQVVD
                     HHANDATGFSATVLFDTETNSYTLSFRSSEYAIDNNGGDRSRDILGADAEIKSSGFAF
                     AQLVSMTRYFEDLQQGKKSDGTVDPSLAAFFGNSQNQLNVTGYSLGGHLATVFTELYA
                     DRVAQTYTFNGAGRGEFAGLHFNSEAQEADRIREMLVNLDARLRATDPLGSLFASGAT
                     ADIYTDERYLVALDDIRARYPTSGTQSLPGLTGGLTRTDGAFGKIQQLFGHAQTGQDV
                     EVVANSGVHAKVIPVLVEGQPLIEDVNVQNPWESQYGNSHSVTLLVDSLAIQELFQKA
                     DPQLQQSQIESILKASSDQIASPYPEQGTPVPPAEGDTLEKALDALAKVFHVEGPATP
                     YGRLPGDFGSATYRQPFYERLDAVRAAIGDQVFSIAPLIGKSAEELQALALLEDSTSL
                     AYRFALRELAPFAVLGASASSTEALYASHNEAGQLALLNSESGMGELSPQYLKDRAAF
                     LVEKVNVNSSPIGFPSITHFKDFQSLYEIRSVAPQGPQVLFGGEVGETLDGSAFFGDH
                     LYGGDGDDHLFGYGGKDYLEGNPGNDILDGGSGADTMLGGTGDDTYLVDDSGDVVREY
                     ANSGIDEVRSSVTLTLDSQIEHLTLTGSNAIDGIGNELGNILIGNSAKNMLNGGGGQD
                     HLVGNEGNDTLIGGAGDSDLLEGGVGFDTYIYRSGDGVDRIEDSDAQGQIIFDDHLLQ
                     GGIRRAGDAANSYTSLDGRTTYVMSGTDLIVNGGLIVNENFQSGQMGIQLRDLSPIPS
                     DTGLPAGPFGHVLEGGSGDETLIATNPYSYAMYGNEGNDVLTSQVPSIHDSFSDLRDG
                     GAGDDILVGAGGNDYLVGGSGDDYADVSDGDIFFGGDGNDIAVTDTEIANYAWTHIGS
                     GTHYIDGGAGNDVLLGALGVDVLLGGEGEDVLRGENRPAGWIAKVYGVDLIPQSFPML
                     AFSSTLGADDYLDGGAGNDLLVGDGGNDILIGGAGDDLLYGESDFAQTVPGDDWLDGG
                     EGNDRLFGGAGADSLSGGDGDDLLIGDFSEDPGSADILDGGAGADELQGGGGDDILYG
                     GVGMDRLAGFAGNDVLDGGADSDELQGGAGDDSLWGGLGHDLMTGQEGDDELFGDGGD
                     DELHGEVGNDTLFGGVGNDALFGQDGEDLLSGDAGDDVLIGGLGNDQLDGGDGIDDVQ
                     GREGDDLMAGGTGDDFLYGDGNDPTVLNLLGGNDTLDGEEGDDQLWGGAGQDQLFGGE
                     GTDQLVGDVGDDALFGDAGNDSLFGDSPLFAGQAGNDVLDGGDGNDVLHAGGGEDDLH
                     GGAGNDRLIGGAGSDTYRFNLGGGIDSIEDDATQSNRLVFGAGITAESLRLDVATGDS
                     LVVRVGNSEDAVQIVGFGLNSPSEFHTINQFEFADGMVLTDAGLLARGFNLSAPVDGG
                     TMIGTSFADHMQGSQAADWFHGRDGNDVLIGGEGADVLEGEEGDDRLEGDAGNDRLYG
                     GAGVNVLRGGEGSDLLVSNAADDQLFGGIGDDIYYLLSGLATVSEDANAGVDTIQLAP
                     SASLAFQAPDQVENVRILDDFYLDPTQRVDLVGNVLDNQLSGPNKLDGQGGNDVLIGV
                     GDNTFLFDRGYGQDIVRTGMQTYAHTGLDQVLFGAEIAPGDLILENHVNDLVVKVNGS
                     TDQLTVESFFVSPSNNVDQFAFFDGTVWDLSAIESRVLTFVGSDADDTFYGTDVDNII
                     RGLGGNDQIRASLGNDILDGGAGNDFLEGYLGNDIYLFGRGYGQDSIDDQGDSSDVDT
                     LQLLDGITPGDVTLRATQDFGIDAILTINNTGDQLTLGGFFAFQSLRVDLIEFADGTV
                     WDYNAMLAHTEGVNLVGGEDVDYLYGNVTNDILSGLGGDDSLYGGAGNDLLDGGIGAD
                     GMIGGSGNDLYTVDDVGDGVTEQAGQGTDTVQSSVTYALGANVENLTLIGNAAINGTG
                     NSLNNILTGNGAVNVLTGGAGNDTYVVGAGDTIVEAAGSGTDTIRADISWTLGTNVEN
                     LVLTGTAAVNGSGNSLANTLTGNSAANILNGGTGADKLIGGQGDDTYVVDNVSDQITE
                     SANQGLDTVQSSVTYTLAANVENLTLTGTGAINGTGNGLDNILTGNNAANRLTGGAGN
                     DTYVIGAGDSIVESVNAGIDSVQSSVTHALAANVENLTLTGSALINGTGNGLNNILIG
                     NSAVNTLTGGAGHDTYIVGVGDTVVEASNAGTDTVQSAVTWSLGANVENLALVGSAAV
                     NGTGNSLANVLTGNVGNNSLVAGDGNDTIAGGQGNDVLNGGTGNDVYQFARGDGQDTV
                     TDTSGSSDRLNFGSGINPLDIMLSQSANDLRIALYGSTDQVTIANWYGGATNQIETVQ
                     AGNGQQLMSTQVNQLIQAMAGFTQQTGLSWEQAVAQRPQDVQQVLAANWH"
BASE COUNT         1698 a         2351 c         1759 g         1628 t
ORIGIN      
        1 caactctcca caatatcggg ctattcatac acggtcggca ggaaaaagga gggtctgccg
       61 gggattggcc ctgactatgt caagacaggg cgcgaaaaac gaggcgctca agagggagtc
      121 gccccgccgg acaggcggag cgaccgaaac ataccgctag tgccaattcg cagccagaac
      181 ctgctgcaca tcctgggggc gctgggccac ggcttgctcc caactcaacc ccgtctgttg
      241 cgtgaatccc gccatcgcct ggatcaattg gttcacttga gtactcatca attgttgacc
      301 gttaccggct tggactgttt caatctgatt agtagcgcct ccataccagt tggcaatcgt
      361 cacctgatcg gtcgaaccgt agagcgcgat gcgcaaatca ttggcacttt gactcaacat
      421 gatgtccaga ggattgatcc cggagccgaa gttgagccga tcgctgctcc cgctcgtgtc
      481 agtcacagta tcctgaccat ccccgcgagc gaattgataa acatcatttc cagtcccacc
      541 attgagcaca tcgtttcctt gtccacctgc aatggtatcg ttaccgtcac cggctaccag
      601 tgaattatta ccgacattgc cggtcagcac attggccaga gaattgcccg tcccgttgac
      661 cgctgcagat ccgaccagcg ccagattttc cacattggct cctaaactcc aggttacggc
      721 gctctgaacc gtgtcggtgc cggcattcga cgcctccacc accgtatctc ctactcccac
      781 aatatatgtg tcgtgccccg ccccaccggt caacgtgttg actgcactat tgccgatcaa
      841 gatgttattg agcccgttcc cagtgccgtt gatgagagca cttcccgtaa gcgtcagatt
      901 ctcgacattt gcggcaagcg cgtgcgtgac actactctgg accgagtcga taccggcatt
      961 caccgattcg acgatgctat cgccggcccc gatcacatag gtgtcgttcc cagcgccgcc
     1021 agtgagtcga ttggcggcat tgttcccggt cagaatattg tccaacccgt ttccggtccc
     1081 attgatggct ccggtcccgg tcagcgtcag gttctcgacg ttggcagcca gggtgtacgt
     1141 taccgaactc tgaacggtgt cgagaccttg attggccgac tcagtaatct gatcgctaac
     1201 gttgtccaca acataggtgt cgtcgccctg cccgccaatc aacttatccg cgccagttcc
     1261 tccattgaga atattagcgg cactgttccc cgtcagagta ttggcgaggc tgttgccgct
     1321 tccattgact gcggcggttc ccgtcaacac aaggttttcc acattggttc ccaaggtcca
     1381 gctgatatcg gctcggatgg tgtcagtccc tgagcctgcg gcttcgacaa tcgtatctcc
     1441 cgccccaacg acatacgtat catttcctgc gccgccagtg agaacattga ccgcgccatt
     1501 gccggtcaag atattgttga gggagtttcc agtgccgtta atggccgcat tgccgataag
     1561 cgtaagattt tcgacgttgg cacccagcgc atacgtgacg ctgctctgaa ccgtatccgt
     1621 tccttgtcct gcctgttccg tcactccatc tccaacatca tcgaccgtat aaagatcatt
     1681 tcctgacccg ccgatcatgc catccgcacc aatccccccg tccaataggt cgttgccggc
     1741 cccaccatag aggctgtcat ctccacccaa tcccgaaaga atgtcgttcg tgacattgcc
     1801 gtagaggtaa tcgacatcct caccgccgac gagattcacg ccttcagtgt gagccagcat
     1861 cgcgttgtaa tcccagacag tgccgtctgc gaattcgatc aggtcaacac ggagcgattg
     1921 aaacgcgaaa aatccgccga gagtcagttg atcgccggta ttgtttatcg tcagaattgc
     1981 atcgatgccg aaatcctggg tcgcacgcaa cgtgacatcg ccaggggtaa ttccgtccaa
     2041 gagttgcagc gtgtcgacat ccgatgaatc gccttggtcg tcgatggagt cctgcccata
     2101 tcctcgacca aacagataga tatcattgcc aagatacccc tccagaaaat cgtttccggc
     2161 gccaccatcg agaatatcgt ttcccagcga agcccggatt tgatcgttcc ctcctagccc
     2221 acgaataata ttgtctacgt ccgtgccgta gaacgtgtcg tcggcgtcac tgccgacaaa
     2281 cgtcaacact cgactctcga tcgcgctcag atcccacacg gtcccatcga aaaacgcaaa
     2341 ttggtccacg ttattgctcg gcgagacaaa aaaagactcc accgtcaatt gatctgtgct
     2401 cccattgacc ttaacgacca gatcgttgac atgattttcg agtatgagat cgcccggcgc
     2461 gatctccgct ccgaacagaa cctgatcgag ccctgtgtgg gcatacgtct gcatgcccgt
     2521 ccgcacgatg tcttgcccat acccacggtc aaacaagaac gtattgtcgc ccaccccgat
     2581 gagaacgtcg tttcctcctt ggccgtcaag tttgttagga ccggacaatt ggttatccag
     2641 cacatttcct accaggtcca ctcgctgggt cggatccaaa taaaaatcgt ccaggatccg
     2701 aacattctcc acctggtcag gcgcttgaaa tgcgagagaa gcactgggcg ccagttggat
     2761 ggtatcaacg cccgcattcg cgtcctcgga cacggtggca agtcctgaga ggaggtagta
     2821 gatatcgtcg ccgatcccac caaataactg atcgtcggcc gcgttcgaca ccaacagatc
     2881 actgccctcg ccaccccgca atacgttcac cccggcgccg ccatacagtc ggtcatttcc
     2941 ggcgtcgcct tctaatcgat cgtcgccctc ctccccctcc aacacatcag cgccctcgcc
     3001 gccaatgagg acatcgtttc catcacgtcc gtggaaccaa tcagcagcct gactgccttg
     3061 catatggtcg gcaaaggacg taccgatcat cgttcctccg tccactggtg ccgacaagtt
     3121 aaaccctcgc gcgagcaatc cggcgtccgt cagcaccatg ccatcggcaa attcgaactg
     3181 attaatggtg tgaaactcgg aaggtgaatt cagcccaaag ccgacgatct gaaccgcgtc
     3241 ctcactgtta ccgacacgta cgacaaggga atcaccggtt gccacgtcca acctcagaga
     3301 ttcagcggta ataccagcgc caaagaccaa tcgattactc tgcgtcgcat catcctcaat
     3361 agagtcgata ccgcccccca ggttaaatcg atacgtgtca ctcccagctc cgccgatcag
     3421 ccgatcattt cctgccccac cgtgcagatc atcctccccg ccacctgcat gaaggacgtc
     3481 attgccatca ccgccatcga gcacgtcatt ccctgcctgt cccgcaaata gcggcgagtc
     3541 tccaaagaga gagtcattac cggcatcccc gaacaaggca tcatctccca catcgcccac
     3601 caactggtcg gttccctcgc caccgaacag ttgatcctgc cccgcaccac cccacaactg
     3661 gtcatccccc tcctcgccat ccagagtatc gttgccgccg agaagattca gaacagtggg
     3721 gtcgttgcca tcaccataga ggaaatcgtc gccggtgccg ccggccatga gatcgtctcc
     3781 ctctcggccc tgcacatcgt caatcccatc gccgccatca agctgatcat tgccgagtcc
     3841 accaatcaac acatcatcgc cggcatcgcc ggagaggaga tcctcaccat cctgaccaaa
     3901 gagcgcatca ttgccgacgc caccgaaaag cgtatcgttc ccgacctccc catgcaactc
     3961 gtcgtcccca ccatcaccaa agagttcgtc atctccctcc tgcccggtca taagatcatg
     4021 accaaggcca ccccacaatg aatcgtcgcc ggctcctccc tgtagctcgt cgctgtccgc
     4081 accgccatcg agaacatcgt tcccggcgaa cccggcgagc cgatccatcc cgactccacc
     4141 atagagaata tcgtcgcccc ctccaccctg gagttcgtca gctccggcac cgccatcgag
     4201 aatatccgca gacccgggat cctcggaaaa gtccccgatc aaaagatcgt cgccatcccc
     4261 accggaaaga gagtcagctc ctgcgccgcc gaatagtcgg tcattgccct caccaccatc
     4321 caaccaatcg tcgccaggaa cagtctgggc aaaatctgac tcaccataca gcaagtcgtc
     4381 gcctgccccg ccgatgagaa tgtcgtttcc tccgtcgccg acaagcaggt cgttaccggc
     4441 gcctccatcg agatagtcgt ccgcacctag agtcgaggaa aatgccagca taggaaaact
     4501 ttgtgggatg agatcaacgc catatacttt tgcaatccaa cccgccggac ggttctctcc
     4561 cctaagaaca tcctcaccct ccccaccaag cagcacatca acgccaaggg cgcccaataa
     4621 cacgtcattg ccggcgcccc cgtcaatata gtgtgtgccg ctgccgatat gggtccatgc
     4681 gtaattagct atctcggtat cagtcacagc aatgtcattg ccatctccac cgaaaaaaat
     4741 atccccatcg gagacatcgg cataatcatc accgctaccc ccaacgagat aatcattccc
     4801 tccagcaccg actaagatgt catcaccggc cccaccatcg cgaaggtcag aaaacgagtc
     4861 atgtattgac ggcacctgag aggtgaggac atcatttcct tcgttgccat acatggcata
     4921 cgaatacgga ttcgtggcaa tcagcgtctc atccccgctc cctccctcca acacatgccc
     4981 aaaaggtcct gcaggaagtc ctgtatcaga cggaatgggg gaaagatcgc gcaactgaat
     5041 ccccatttga ccgctctgga aattctcatt cacgatcagg cccccgttca cgatgagatc
     5101 ggtcccggac atcacgtaag tggttcggcc atccaggctc gtataactgt tggcggcatc
     5161 accggcgcga cgaataccgc cttgcaacaa gtggtcatcg aaaatgatct gcccctgcgc
     5221 atcactgtct tcaattcgat ctaccccatc accagaacga tagatgtacg tatcgaatcc
     5281 aacgccgcct tcgaggaggt cgctgtcgcc agcgccgccg atgagtgtgt cgtttccctc
     5341 attgccgact agatggtcct gcccaccacc accattcagc atattttttg cgctattgcc
     5401 gatgagaata ttgcctagct cgttgccaat gccatcaatc gcattgctcc ccgtcagcgt
     5461 gaggtgctcg atctgggagt ccagggtcaa ggtgactgaa ctgcgcactt cgtcaatgcc
     5521 gctattggca tactctcgga cgacatcgcc agaatcgtcg acgagatacg tgtcgtctcc
     5581 cgttcctccc agcatcgtat cggcgccact gccgccatcc aagatgtcgt tgccgggatt
     5641 cccctccaga tagtccttgc ctccatagcc aaacagatga tcgtcgccat caccgccata
     5701 gagatggtca ccaaaaaacg cactcccatc gagagtttcg ccgacctcgc ctccaaacaa
     5761 gacttgaggc ccttgtggtg ctaccgaacg aatctcgtac agtgattgga aatctttaaa
     5821 atgagtgatg gaggggaaac cgattgggct gctgttgaca ttgaccttct cgaccagaaa
     5881 tgcggcgcga tccttcaagt actgtggaga caattccccc attccgcttt cggagttgag
     5941 cagtgccaac tgtccggctt cgttgtggct agcatagagt gcctctgtcg aggaagcgct
     6001 agccccgagg accgcaaatg gggctaactc ccttaatgcg aaccgataag caagactcgt
     6061 tgaatcctcc agtaaggcca aggcttgtaa ctcctcagcg ctcttcccta tcaatggcgc
     6121 aatgctgaag acctggtcac cgatcgccgc tcgaacggcg tcaagtcgct cataaaacgg
     6181 ctgtcgatac gttgcgctgc cgaaatctcc gggcagtcgc ccgtaaggag tagcaggtcc
     6241 ttcaacgtgg aacaccttgg ccagtgcatc aagcgctttt tcaagcgtgt ctccctcggc
     6301 agggggcaca ggcgttcctt gttctggata gggagaggca atctgatctg aggaggcttt
     6361 cagaatgctt tctatctggg attgctgtaa ctgcggatca gccttctgaa atagttcctg
     6421 gatggccagg gagtccacaa ggagagtgac gctgtggcta ttgccatatt gagattccca
     6481 tggattctgg acattcacat cttcaatgag tggctgccct tcgactaaaa cagggatcac
     6541 ctttgcgtgg accccagaat tggcgaccac ctcaacatcc tgaccggtct gggcatgacc
     6601 aaagagctgc tgtatttttc caaaagcacc atcggttctc gtcagccctc cggtcagacc
     6661 aggcaaactt tgagttccac tcgtgggata gcgcgcccta atgtcatcca acgcgaccag
     6721 ataccgttcg tcggtgtaga tgtcagccgt cgccccactg gcaaacagcg agcctaacgg
     6781 atcagttgcc cgtaaccgcg catcaaggtt cacaagcatc tcccgtatgc gatcggcttc
     6841 ctgtgcctcg ctgttgaaat gtaaaccggc aaattcaccg cgcccggccc cattaaaggt
     6901 gtacgtttgg gccacgcgat ccgcatagag ttctgtgaat acagtcgcta ggtgcccgcc
     6961 gagcgaatag ccagtgacat taagctggtt ctgggaattg ccaaagaagg cagccaatga
     7021 cggatcgacg gtcccatcgc tcttcttgcc ttgttgcagg tcctcaaaat atcgcgtcat
     7081 actgacaagt tgcgcaaatg caaatccgga gctcttgatt tccgcatctg cgcctaaaat
     7141 atcgcgggaa cgatcaccac cattgttgtc gatggcatac tcgctgcttc gaaatgaaag
     7201 cgtgtagcta tttgtctcag tatcgaaaag tacggtagca ctgaagccag tcgcatcgtt
     7261 ggcatggtga tcgacgacct gatagcgctg gacgaattgc tcagcctgca gcctcgtcat
     7321 ccgggtcttg ccaggcaaca cgagtgccgt cggatcatca ccagttatat tgttgtttcc
     7381 aagttgaagt tggccgatca cccgttgagg actcgataga tcaatcccgt caaggt
//
LOCUS       DAORRQ010000120         7384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_395_length_7384_cov_22.132760, whole genome shotgun sequence.
ACCESSION   DAORRQ010000120 DAORRQ010000000
VERSION     DAORRQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_395_length_7384_cov_22.132760"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /gene="metG"
                     /locus_tag="PK224_19900"
     CDS             <1..377
                     /gene="metG"
                     /locus_tag="PK224_19900"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRB18068.1"
                     /translation="AAPSPAPATAAPAAAQPAQIGIDDFMKIQLKAAKVLSAERVPKS
                     EKLLKLQVSLGSEQRQIVAGIGKKYEPETLIGKTIVIVANLKPAKLMGIESQGMVLAA
                     GDSEVRGLATFIEDVEPGTKVK"
     gene            374..1240
                     /locus_tag="PK224_19905"
     CDS             374..1240
                     /locus_tag="PK224_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18069.1"
                     /translation="MSRPFFIRLIGTCLLVLLSSAGAAFTPDPSDSELESKTHYNDPL
                     PTTVLVRVVAHRSLVLGHDVGGARVTITDMASGQLLASGLQQGEPGDQTQIMRTPHLM
                     DEPVYSGRPAASFSATLELTRPTLVEITAEGPLAYPNALQRASTTVLLIPGHDLTSDG
                     IVLHLYGYLVQVEHPKPGEPLIAKEDVMLRASVRTLSGALVRPHSDWDSRKIHIYAEL
                     LIGDRIIERLQMFYAGEKSRFEAPFFVPMSKDAPDGITLRVIAADTATGNFGVGTAQY
                     PVLSERLRPRKN"
     gene            1328..3112
                     /gene="ftsH"
                     /locus_tag="PK224_19910"
     CDS             1328..3112
                     /gene="ftsH"
                     /locus_tag="PK224_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRB18070.1"
                     /translation="MDPKQRQFSIWYMFIALWALILLQTFLPSFFNPTEIPYSEFKES
                     VAAGKVTEVAVSPQIIHGKLKEDKVFHTIRIEDPDLLRNLAEHQVKVTGVIESTLFRD
                     VLSWIVPIVLFFGVWWFLLRRMGQSQGFMTVGQSKAKIYVEKEVKVTFADVAGVDEAK
                     QELEEIIEFLKTPEKFRRLGGKIPKGILLVGPPGTGKTLLAKAVAGEAGVPFFSISGS
                     EFVEMFVGVGAARVRDLFEQAKGKAPCIIFLDELDALGKARGVGPMAHEEREQTLNQL
                     LVEMDGFDSRVGVILVAATNRPEILDPALLRAGRFDRQVLVDRPDKIGRLAILKVHAR
                     TITIANQADLETIAAMTPGFVGADLANLLNEAALLAVRRGKDTVSLSELQEAVERVIG
                     GLEKKNRVLNKMERARVAHHEVGHALVAMSIPGGDAVHKISIIPRGIAALGYTMQLPT
                     EDRFLMTVSELKNRIAILLGGRAAEEVIYGEVSTGAQDDLRKATDIAKSMVKAYGMSE
                     KLGQVSLERDRQSIFLQTGPSQTPGDYSEQTSREIDCEVRLLIDEQFERARNLITSQE
                     AILRKAAQVLLEKETISGEELKALAASH"
     gene            3250..4191
                     /locus_tag="PK224_19915"
     CDS             3250..4191
                     /locus_tag="PK224_19915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRB18071.1"
                     /translation="MVPHTYHELRSRLRIALALNAVIIVAEFVGGFLTNSIGLIGDAG
                     HNLVDQGSLFLALYAHILTARPATDSRTFGYHRAGIVAAFLNSFILLLTAVGITIMSA
                     QRILTPVPIPGGWVMLIALISFVANLSIALLLQHGAKDDLNIRSAFWHMLSDAWVSLG
                     VVVSGGAIMLTGWSVLDPLVSLFVVVAIIRGAWPLFKESLEVLLESTPPGVSPAQVAA
                     TIESIPGVKNVHDLHIWAVEPRLIMMTTHVQVDGHDEALTSDLLHTIRERITTDFGIK
                     HLTIQLETECCHPEAVHCDLNKLAGQRSHPEFLHSHH"
     gene            complement(4345..5154)
                     /locus_tag="PK224_19920"
     CDS             complement(4345..5154)
                     /locus_tag="PK224_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18072.1"
                     /translation="MVVVVGAGAGLAWTENLNQRQKAVILSLCLLCAMLCIVGVIWCL
                     YTLKQIKYRVVVGGEGIARIPESAPAVSVGWSDIVAVEDRMRANRLRLYGVGKRLLME
                     FDWQLENADKLEEVIHQKVYRDRLVHEGVKRFRVRKGANTFGVLPPIGFVWFSLLSGM
                     QGAYIPSAVFAAMAVGALLSWGNLIQEVAIMAEGLQLRTLLKLRSIGWPEISGIDMST
                     VKDERPGGSGRAYRVVKVLLKNGKPIRMPPVQEGNLLLFDALSYEHRVSQS"
     gene            5368..6405
                     /locus_tag="PK224_19925"
     CDS             5368..6405
                     /locus_tag="PK224_19925"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRB18073.1"
                     /translation="MSQSIKSFVNKTDTSLIPDSYNSRMTQPQTLPVPFYVLCGSLGA
                     GKTTLLMRLLEFWNSQGHKVGVLMNEAGEVSIDGPRAGTIAEQVLNLAGGCICCDTKD
                     DLAWSITQLVQDYQSTVIVLECSGMADPAEVVDAVTDAYVSRIAKLERIFAMLHPVPL
                     PDSGMAELVTRNAIRYADDLILNKRDLYIPGHWEQFRETITHHNPYGRIWETNHARLD
                     ISALLAAPATRTVPTNVSFGKPPTADERHNARAPHHPMVTTVRLPKPLDRKKFMEWTK
                     SLPEGMERAKGFFRFAGEPELQEFQFFPPRTSTVAPVMLLDEPDHVVVLIGRAYDQEG
                     CRASLLACLAS"
     gene            complement(6411..6998)
                     /locus_tag="PK224_19930"
     CDS             complement(6411..6998)
                     /locus_tag="PK224_19930"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRB18074.1"
                     /translation="MGLDETLFHSINGVAGRSSLLDWVMVELAKPGNLLYPILLAAAY
                     WFWKNQRECLIGAAALAGVIGAADALGTQLKGLVQRPRPCVTLADVHQLLGCGGAFSF
                     PSNHAANTAAAAAFFQVLYPKSGWIGWPLVAAIGISRVYIGAHYLTDVMGGWVVGGLI
                     GAGAAWSLTRWSRFRPRAERMAPAGVRQPSAGSIS"
BASE COUNT         1628 a         2361 c         2019 g         1376 t
ORIGIN      
        1 ctgcagcccc aagcccggcc cctgcgaccg ccgcccccgc tgccgcgcaa ccggcgcaga
       61 tcggcatcga cgacttcatg aaaatccaac tgaaggcggc gaaagtcttg tcggcggagc
      121 gggttccgaa atccgaaaag ctgctcaagc tccaggtcag ccttggcagc gaacagcgac
      181 agatcgtcgc cggtatcggc aaaaagtacg agccggaaac actgatcgga aaaaccatcg
      241 tcatcgtagc gaatttgaaa ccggccaaac tgatgggcat cgaatctcag ggcatggtcc
      301 tcgcggcggg cgacagcgaa gtgcgcggac tcgccacctt catcgaagat gtggagcccg
      361 gcaccaaagt gaaatgagtc gccccttctt catccgactg atcggcacct gccttctcgt
      421 gctgctgagc agtgcgggcg cggcattcac gcctgacccc tccgactccg agctcgaaag
      481 caaaacccac tacaacgatc ccctccccac caccgtgctc gtgcgagtcg ttgcgcaccg
      541 ctcgttggtc ctgggccacg acgtcggcgg cgcgcgcgtc acgattaccg atatggcgag
      601 cgggcaactc ctggcctccg gcctgcaaca gggagagccg ggcgaccaga ctcagatcat
      661 gcgcacgccg cacctcatgg acgaaccggt ctacagcggc cgtccggcgg cgtccttctc
      721 cgccaccctc gaactcacgc gcccgacgct cgtggaaatt accgcagagg gaccgctggc
      781 ctatcccaat gccttgcaac gggcgagcac gacggtcctc ctgatccccg gtcacgacct
      841 gacgagcgac ggcatcgtgc tgcacctcta tggttacctg gtgcaggttg agcatccgaa
      901 accgggtgag cccctcatcg ccaaagaaga tgtcatgctg cgcgcctcgg tacgcaccct
      961 ctcgggagcg ctggtccgcc cgcacagcga ctgggattcc cggaagattc acatttacgc
     1021 cgaactcctg atcggcgacc ggatcataga gcgcctgcaa atgttttatg cgggcgagaa
     1081 gagccgcttc gaagcgccgt tcttcgtgcc gatgtccaaa gacgcgcccg acggcatcac
     1141 cctgcgcgtc atcgcagccg acaccgccac cggcaacttc ggggtgggaa ccgctcaata
     1201 cccggtgctc tccgagcggc tcagacccag gaagaattaa ttcaggcgtg gtatggtgac
     1261 taagtaacgg acgaatatct ccgttcaatc accggatcac ccgaatactc aatcgtacgt
     1321 tctgcttatg gatcctaaac aacggcaatt ctccatctgg tacatgttca tcgccctctg
     1381 ggcgctgatt ctgctgcaga cgtttctccc ctcgttcttc aacccgaccg aaattcccta
     1441 cagcgaattc aaggaatccg tggcggcggg gaaggtcacc gaagtcgcgg tctcgccaca
     1501 gatcattcac gggaaactga aagaggacaa ggtcttccac acgattcgta tcgaagaccc
     1561 ggatctgctc aggaatcttg cggaacatca ggtgaaggtc accggcgtca tcgagagtac
     1621 gttgttccgc gacgtgttgt cctggatcgt gccgatcgtg ctcttcttcg gcgtctggtg
     1681 gttcctcttg cgccgcatgg gtcaaagcca gggcttcatg acggtcggcc agagcaaagc
     1741 caagatctac gtggaaaagg aagtgaaggt gacgtttgcc gatgtggccg gggtggacga
     1801 ggccaagcag gagctggaag agatcatcga gtttctgaag acaccggaga agttccgccg
     1861 gctgggcggc aagattccaa aaggcattct gttggtcggc ccgccgggca ccggaaaaac
     1921 ccttctggcg aaggccgtgg ccggagaagc cggcgtgccg ttcttctcca tcagcggctc
     1981 ggagttcgtg gagatgttcg tcggcgtcgg tgcggcccgc gtgcgggacc tgttcgagca
     2041 ggccaaaggc aaggccccct gcatcatctt cctcgacgag ctcgacgcgt tgggcaaggc
     2101 ccgcggggtc ggtccgatgg cccatgaaga acgggaacag acgttgaatc agttgctggt
     2161 cgaaatggac gggttcgact cacgcgtcgg ggtcattctc gtggccgcca ccaaccgccc
     2221 cgaaattctg gatcccgcgc tgctgcgggc cgggcgattc gatcggcaag tgctcgtaga
     2281 ccggccggat aaaataggac ggctcgcgat tctgaaagtc catgcccgca ccatcacgat
     2341 cgccaatcag gcggatctgg aaaccatcgc cgccatgacg cccggctttg tcggcgccga
     2401 tctggctaat ctactgaatg aagcggcgct gctcgcagtt cgacgaggca aagacaccgt
     2461 cagcctgtct gagttgcagg aagccgtgga gcgagtcatc ggcgggctcg aaaagaaaaa
     2521 ccgcgtcctc aataaaatgg aacgagcccg ggtcgcccat cacgaggtcg gccatgcgtt
     2581 ggtggccatg tccatcccgg gcggcgatgc ggtgcacaag atctcaatta ttcctcgagg
     2641 tattgccgcg ctcgggtaca ccatgcagct cccgaccgaa gaccggttcc tcatgaccgt
     2701 gtcagaactc aagaaccgga tcgcaatcct cctgggtgga cgggcggcag aagaagtcat
     2761 ctatggcgaa gtctcgaccg gcgcgcagga cgatttacga aaggccaccg atatcgccaa
     2821 gagcatggtc aaggcctacg gcatgagcga gaaactcggc caggtgagcc tggagcggga
     2881 tcggcaatcc atcttcctgc agaccggtcc ctcgcagaca ccgggggact atagcgagca
     2941 gacttcgcgc gaaatcgatt gcgaagtccg gctcttgatc gatgaacaat ttgaacgagc
     3001 ccgcaacctc atcacatccc aggaggccat cctgcggaag gcggcacagg tgctgctgga
     3061 aaaagaaacg atcagcggag aagaactcaa ggcactcgcc gcatcgcact gaagccggtc
     3121 cgaactgaag cgcgatcgcc acgtccgaca gcacgatcca accgtcaccg caacgcgtcc
     3181 ctcctgctcc cgcaacagga ccgtgacaac gtcttgcccc cgtcggtctc tcatgagaca
     3241 ctgactacga tggttcccca cacctaccat gaactccgct cccgactgag gatcgcgctc
     3301 gccctcaacg ccgtcatcat cgtcgcggag tttgtcggcg gcttcctcac gaacagcatc
     3361 ggtctgatcg gagacgcggg ccacaacctc gtcgatcagg gctccctgtt cctggcgtta
     3421 tacgcgcaca tcctgacggc gcgccccgcc accgacagcc ggaccttcgg ttatcaccgg
     3481 gccggcatcg tggccgcatt cctcaactcc tttatcctgc tgctcactgc cgtcggcatc
     3541 acgatcatga gcgcccagcg catcctgact cctgtcccga ttcccggcgg ctgggtcatg
     3601 ctcatcgccc tcatcagctt tgtcgccaat ctgtcgatcg cactcctgct ccagcacggg
     3661 gcgaaagacg acctcaatat ccgcagcgcc ttctggcaca tgctgagcga tgcctgggtc
     3721 tcattggggg tcgtggtgag cggaggggcc atcatgctga ccggctggtc cgtcctcgac
     3781 ccgttggtaa gcctcttcgt cgtcgttgcg atcattcgcg gcgcctggcc gctctttaaa
     3841 gaatcgcttg aggtcctcct ggagtccacc ccgcccggcg tcagccctgc gcaagtcgcc
     3901 gccaccatcg aatccattcc cggcgtgaag aatgtccatg atctgcacat ctgggccgtt
     3961 gagccgcgcc tcatcatgat gaccacgcat gtacaagtcg atgggcacga tgaggccctg
     4021 accagcgacc tcctccatac catccgagag cgcatcacaa ccgacttcgg catcaagcac
     4081 ctgacgatcc agctcgaaac cgaatgctgc catcccgaag ccgtacactg cgacctcaac
     4141 aaactggccg ggcagcggtc ccatcccgag ttcctgcaca gccatcatta agactccccc
     4201 caaatttctg ggccatccct accagtagaa tccgcgagtg cgttggctgg gcagtcaatc
     4261 tggacgggcc ggtatttctt agacagcctt ggaggaaccg aacggaaata gaagtgaggt
     4321 cgatggtacg gcggtgtcga gatactagct ctgactcacc ctatgctcat agctcaatgc
     4381 atcaaacaat aggagatttc cttcttggac cgggggcatc cgaatgggct ttccattctt
     4441 caatagcact tttacaaccc gataggctct ccccgaccca ccaggtcttt catccttgac
     4501 tgtagacata tcaatcccgc tgatttctgg ccagccaatg cttcgaagct tcagcaacgt
     4561 tcgcaactgg agaccttcag ccatgattgc cacctcttgt ataagattgc cccatgaaag
     4621 aagagcgccc accgccatcg cagcaaaaac ggcactggga atataggcac cctgcatccc
     4681 agagagaagt gaaaaccaaa cgaatccgat aggtggcaaa actccgaacg tgttggcacc
     4741 cttccgcaca cgaaaacgct tcaccccctc gtgaacgagc cgatctcgat agaccttttg
     4801 gtggatcact tcctcaagct tatccgcatt ctccagctgc caatcaaact ccatcaggag
     4861 acgctttccc actccgtaga gcctcaaacg attcgcacgc attcgatctt ccacggcaac
     4921 gatatcactc caaccgactg ataccgctgg cgcagattcc ggaattctcg caattccctc
     4981 cccccccacc acgaccctgt acttaatctg cttaagcgta taaaggcacc agataacccc
     5041 gactatgcaa agcatcgcgc acaagagaca taatgacagg attaccgcct tttggcgttg
     5101 attaagattt tcagtccaag ccaatccggc tccagcgccg actacaacca ccatacatat
     5161 cgctataatg atcgcaccca ccagggaaga cctgaatgac tttgagatac gaaagactgt
     5221 ttcgtccatt ccagagaacc tcggcaaaca attaacacgt gcctgatgag gctatgcgct
     5281 cacctttctt agtaacactc cgccgttttc tacgcattgg aaatagagaa aagccacaac
     5341 ttcctatcgc tccctcatgc aggtagaatg tctcaatcca taaaatcgtt cgtgaacaag
     5401 actgatacct ctctcattcc agacagctac aatagccgca tgactcagcc acagacactg
     5461 cccgttccct tttacgtcct gtgcggatcg ctgggcgccg gcaagacgac gctgctcatg
     5521 cgcctcctcg aattctggaa cagccagggc cacaaggtcg gtgtgctgat gaacgaggcg
     5581 ggcgaagtca gcatcgatgg gccgcgcgcg ggcacgatcg ccgagcaagt tctcaatctt
     5641 gccggcggct gcatctgttg cgacaccaaa gacgatttgg cctggagcat tacccagctc
     5701 gtgcaggact accaatccac ggtgatcgtg ctggaatgtt ccggcatggc ggacccggcg
     5761 gaagtggtcg atgccgtgac ggatgcctac gtatcacgca tcgcgaagct tgaacggatc
     5821 ttcgcaatgt tgcatcccgt gcccctgccc gatagcggca tggcggaact cgttacgcgt
     5881 aacgcgattc gttacgccga tgacttgatc ctcaataagc gcgacctcta catccccgga
     5941 cattgggaac agtttcgcga gaccatcacg caccacaatc cgtatggacg aatctgggaa
     6001 accaaccacg cgcggctcga catttccgcg ttgctcgccg cgccggcgac acgcacagtg
     6061 ccgaccaacg tgtcgttcgg gaaaccgccc acagcggacg agaggcacaa cgcgcgcgca
     6121 cctcatcacc cgatggtgac cacggtgcgg ctaccaaagc cgctcgaccg caaaaagttc
     6181 atggaatgga cgaagtcgtt gccggaggga atggagcgag cgaagggatt ttttcggttt
     6241 gcgggagaac ccgaactgca ggagtttcag ttttttccgc cgcgcaccag cacggtcgcg
     6301 ccggtcatgt tgctcgatga accggaccat gtcgtggtct tgatcggacg tgcctacgac
     6361 caggaaggct gtcgcgcttc cctcctcgcc tgcctcgctt cataacattg tcaggagatc
     6421 gaaccggctg agggctgtcg cacaccggcg ggagccatgc gctcagcacg cggcctgaat
     6481 cgagaccagc gggtgagtga ccaggccgcg cccgccccga tcaatccccc caccacccat
     6541 ccgcccatca catccgtcag atagtgggcc ccgatgtaga cgcgcgaaat gccaatggcc
     6601 gcgaccagcg gccagccgat ccagccggat ttgggataga gcacctggaa gaacgccgcc
     6661 gccgccgcag tattggctgc gtgattggag ggaaaggaaa aggccccgcc gcagccgagc
     6721 aactgatgta catcggcaag cgtcacacag gggcgcgggc gctgaaccaa gccctttaat
     6781 tgggtcccga gtgcgtccgc cgcaccgatc acgccggcca acgccgccgc tccgatcaga
     6841 cattcccgct ggttcttcca gaaccaatag gccgcagcga gcagaatggg atacagcaga
     6901 ttgcccggtt tggccaattc gaccatcacc caatcaagca gactcgaccg accggcaaca
     6961 ccattgatgc tgtgaaagag agtttcgtcc aatcccatgc cgcgacgata aagggtcggg
     7021 agggatcagt caaatagttc gtgcgttggg gggaggtcaa gaagttgcgg gtcccgtcgc
     7081 cggtccagcc cgtggctggt cacgctcccc ggccacctca ccgactcggc ggcacacaca
     7141 gacttgcgct ctttattcat cgcgccgtgc gccctatccc ccggtggagc ccttacgcct
     7201 cgggcgatcg ggcacccagt tccagcacca cccgcccacc aatcagagat caggcgctga
     7261 gccacgcctg gtatgccaga gggcaagcac ctcaaccacc attggttcag caatcacacg
     7321 gtagtagaga tagtaatgaa tgcgggtgag atgaatgcgg cgaaccccgg tcagcctagc
     7381 gttg
//
LOCUS       DAORRQ010000121         7284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_400_length_7284_cov_51.676580, whole genome shotgun sequence.
ACCESSION   DAORRQ010000121 DAORRQ010000000
VERSION     DAORRQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_7284_cov_51.676580"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PK224_19935"
     CDS             complement(<1..144)
                     /locus_tag="PK224_19935"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRB18075.1"
                     /translation="MNDRFLKACRREPVDCTPVWFMRQAGRYMVEYRRLREKHSILEL
                     CKTP"
     gene            508..1524
                     /locus_tag="PK224_19940"
     CDS             508..1524
                     /locus_tag="PK224_19940"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRB18076.1"
                     /translation="MSSDLQPLTLPENGELSKTSDQPREQPASDGPWITIIRWFDSWA
                     GIEKMQTDTAPPTVDWLRAIPLIIVHLLCLGVFIVGWSWVAVGVAAGFYFIRMFAITG
                     WYHRYFSHRTFKTSRACQFAFALLGASCAQRGPLWWGGHHRHHHIYSDKPEDTHSVRQ
                     GGFLWAHIGWISSKKFFPPRLKSIGDFAKFPELVFLDRFDTLVPTICGFGMFGLGKLL
                     EAYAPSLGTNGPQMLIWGFFVSTVALLHGTCTINSLSHVYGSQRYKTGDDSKNNFILA
                     MITLGEGWHNNHHYYAASTRQGFYWWEIDITYYMLRGLQALGLIWDIREVPAHVREGK
                     NKLA"
     gene            complement(1572..2042)
                     /locus_tag="PK224_19945"
     CDS             complement(1572..2042)
                     /locus_tag="PK224_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18077.1"
                     /translation="MAQKFGNNRWVQEGFLDNRRPGTVVGRMTFAVLGAVDFYLVGDC
                     RGEIAGKVIRFKNSRFVDEDLAGQVLGDVEIPQIGDASLISFDPHPHLVPHPYIEWFS
                     MRKNHYRIELVPEDAWIATEEELVAIDPVSLEIRDRLALQYGRKAASADESEWV"
     gene            complement(2044..2922)
                     /locus_tag="PK224_19950"
     CDS             complement(2044..2922)
                     /locus_tag="PK224_19950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRB18078.1"
                     /translation="MPQFALLLTTFVWGATFPATKAALEQISPLSFLFLRFLLGMTVV
                     FAVLLFFRRPLIRDTSMMRASLIATAWLFIGYVLQTVGLRFTTASNSAFITVLYVVFV
                     PLYLLRLGLHTWVSNGIALAGLWLLVRPTASANLGDLLTLGSAAAFAAHMVCLERYTR
                     VADPVSLFAWQLVLMTVAMSGVMWWEQPTLAMFEPSRVLAVGLVVTGILATGAFAVQM
                     WAQRLLPAQQVALLFAAEPAVAAWLAWYFLGEQLDAQGWFGSAMILGGVLLGSWVTGE
                     SSPAQQESVTARSEGG"
     gene            complement(3029..3232)
                     /locus_tag="PK224_19955"
     CDS             complement(3029..3232)
                     /locus_tag="PK224_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18079.1"
                     /translation="MVYWLHIARAIDRVTLHRDRCSEVPSGVSSSDYWQGGWFDYPDK
                     ERALAAMEQAGTSEERKCALCKP"
     gene            complement(3382..3558)
                     /locus_tag="PK224_19960"
     CDS             complement(3382..3558)
                     /locus_tag="PK224_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18080.1"
                     /translation="MPLLIRKYKGSGGVMQEERIDDPDRIERYMRLFEEKDVKKLGTG
                     VKTVIEKDEWQLLP"
     gene            3717..4034
                     /locus_tag="PK224_19965"
     CDS             3717..4034
                     /locus_tag="PK224_19965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRB18081.1"
                     /translation="MPVYEYRCEQCTHQFEATQSVHARPEDTVCPQCNKIGATRMLSA
                     FASKVKGSHKPGFEEIKAYNMLNERMDRFSKLPPAMGKRIEASPDFMAGAGGPAPTEG
                     HDS"
     gene            4132..4956
                     /locus_tag="PK224_19970"
     CDS             4132..4956
                     /locus_tag="PK224_19970"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRB18082.1"
                     /translation="MHTRISGFVCAALLCLTGLPASAEVAGSMAIAGHGPEQRVIESL
                     AHAFEKANPRAYIDVVWDDNSKPLDLVKTKQAKIAVTGTAEDGLRSFQIAWDGIAIMV
                     HRSNFTKEVTKQEVAELFSGKYKVWADLGGPDTKVLLIDRPRNENNRDAFEQQLGIAG
                     KIPEGAKIIAKDEKVIKTIAGTLPPHSAVSYVSLGQALEAVASGVPVKLLPVDKIEPE
                     TPTVKDGRYTLRRPILLLSHNEPDPLVDAFEQFALSEDGQKIISESYTPLPKTSSP"
     gene            4969..5424
                     /locus_tag="PK224_19975"
     CDS             4969..5424
                     /locus_tag="PK224_19975"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRB18083.1"
                     /translation="MRTIVTCMLGCTLVLGTTFVAVPAGHAQEGAMVEGAGYTMFNTE
                     SIKGSDTSKLESDPVCDRSKRPSIAKVEPDEAKPGEKITIKGENFGSKECFHGVTFSA
                     ASKEKIDYRFVNETTIEAVVPEAKAGMSFIIVVAGGGSAQSKPLLIQPK"
     gene            5598..7016
                     /gene="accC"
                     /locus_tag="PK224_19980"
     CDS             5598..7016
                     /gene="accC"
                     /locus_tag="PK224_19980"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRB18084.1"
                     /translation="MFRKILIANRGEIAMRIIRGCRELNIATAAIYSEADSSGIYVKK
                     ADESYLVGPGPVKGFLDGKQIVEIAKRIGADAIHPGYGFLSENTKFARLCQTSGITFI
                     GPSPETIDLMGSKVKARQIAQQAGVPIVPGTEGGVTSVDEALAFAHQINYPVMIKASA
                     GGGGRGLRVVRSDQELRENIDVASREAQAAFGDGSIFIEKYIERPHHIEFQILGDKHG
                     NIIHLGERDCSIQRRHQKLIEIAPSLILTPKLRAQMGEAAIAIAKAVHYDNAGTVEFL
                     LDHEGHFYFMEMNPRLQVEHTVTEQITAIDIVRNQISIAAGKPLEIRQKDVTLQGHAI
                     QCRINAEDPRNNFMPCTGTITAYLSPGGIGVRIDGAVYRDYTIPPYYDALLAKLTVRG
                     RTWEEAVSRMRRSLEEYVLRGVKTTIPFMKNVMMEQDFQAGRFDTSYLETHPDLYQYE
                     ESEEPEDLVLAISAAIAAYEGL"
     gene            7088..>7284
                     /locus_tag="PK224_19985"
     CDS             7088..>7284
                     /locus_tag="PK224_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18085.1"
                     /translation="MATKKTKKTAPKKSPAKKNVPAKPSRPAASRAVQVTQPASPEFR
                     VTPAPGKKLLMTEVALRDGHQ"
BASE COUNT         1660 a         2314 c         1965 g         1345 t
ORIGIN      
        1 cggcgttttg cacagctcga gaatggaatg cttttcccgt aaccggcgat attcgaccat
       61 gtaacggccc gcctggcgca tgaaccagac cggcgtgcaa tcaaccggtt cgcgccggca
      121 cgctttcaaa aatcgatcat tcataatggg gcgcatttta gaggggcggt ccgaacagag
      181 tcaaacaatc ttgcgccgaa ccggcttgcc ggatgggatc atcctgacac cggaagggcg
      241 gcagctccgg ttctgccgct cgttcgacaa aaaacatggc ggccatgcgg aaaaattctc
      301 aggacatttc cccaggaatc tgtgtataat ggcagcacgt gtctttgagc ctgagtatcc
      361 tcactcactg atcgacacaa ttcagagcat tcagcggcct gcctattcgt atcactcgca
      421 agcgccgcga cgttttagtc acgctagtgg aaccgcggcg cgacccctcg gtaccgtatc
      481 cttgattcgc ccaactgtgg aggtggcatg agtagtgatc tgcaaccact caccttaccc
      541 gaaaatggtg agctctctaa aaccagtgac caaccacgtg aacaacccgc gagcgatgga
      601 ccgtggatca ccatcatacg ctggtttgac tcatgggcgg gcattgaaaa aatgcaaacg
      661 gatactgccc cgcctaccgt cgactggctg cgggccatcc cgctcatcat cgtgcacctg
      721 ctctgcctgg gcgtgttcat cgttggatgg agttgggtcg ccgtaggggt cgcagcgggg
      781 ttctacttta tacgcatgtt cgccatcacg ggatggtacc atcgctattt ttcgcaccgc
      841 accttcaaga cctcccgggc ctgtcagttc gcctttgccc tgctcggggc ctcctgcgcg
      901 caacgcggcc cactttggtg gggcggacac catcgacacc accacattta ctccgacaag
      961 ccggaagaca cacattcggt gcgccagggc ggattcctct gggcccacat cggctggatc
     1021 agttccaaga agtttttccc gccgcgtctg aagagcatcg gcgatttcgc caagtttccg
     1081 gaactggtct tcctggatcg attcgatacc ctcgtcccga ccatctgcgg cttcgggatg
     1141 ttcggcctag gcaagctcct ggaagcctat gccccgagcc tcggcacgaa tggcccgcaa
     1201 atgctcattt ggggcttctt cgtgtccacc gtcgcgctgc tccacggcac ctgcacgatc
     1261 aactcgctgt cccacgtgta tggttcccag cgctacaaga cgggggatga cagcaagaac
     1321 aacttcatcc tcgccatgat taccctcggc gaaggctggc acaataacca ccactactat
     1381 gccgcctcaa cccgccaagg attctattgg tgggaaatcg acatcaccta ctacatgctg
     1441 aggggcctgc aagcgctggg actgatctgg gatattcggg aagtgcctgc gcacgtgcgg
     1501 gaaggaaaga ataaactcgc ctgattgaga ccggggggcg gccgttcacg gcccgccccc
     1561 ttcactccga cctacaccca ttccgattca tctgccgatg ccgccttacg cccgtactgc
     1621 agcgccaggc gatctcgaat ctccaggctg acagggtcga tcgccaccag ttcttcttcc
     1681 gtcgcaatcc aagcatcctc cggcaccaat tcgatacggt agtgattctt ccgcatggaa
     1741 aaccactcga tatacgggtg cgggaccaaa tgcggatgcg gatcgaagga gataagactc
     1801 gcgtcgccga tttgcggaat ctccacgtcc cccagcacct gtcccgcgag gtcctcatcc
     1861 acgaagcggc tgttcttgaa ccggatcacc ttcccggcga tttctccccg acaatcgccg
     1921 accagataaa aatcgaccgc gcccaacacc gcaaacgtca tgcgcccgac gacggtaccc
     1981 ggccgacggt tgtcgagaaa gccctcttgc acccaccgat tgttgccgaa tttctgagcc
     2041 acctcaccct ccttctgaac gcgcagtcac agactcctgc tgcgccggcg acgactcacc
     2101 tgtcacccaa gacccgagca ggacgccacc cagaatcatc gcactcccga accacccttg
     2161 cgcgtccagc tgttcaccca ggaaatacca cgccagccag gccgccacgg ccggttccgc
     2221 ggcaaacaac aaggcaactt gctgcgcagg cagaagtcgc tgcgcccaca tctggaccgc
     2281 aaatgccccg gtcgccagaa taccggtgac cacaagcccg acggccagga cgcggctggg
     2341 ctcaaacatc gcgagcgtcg gctgttccca ccacatgact cccgacatgg ccacggtcat
     2401 cagtaccaat tgccacgcga acaacgaaac cggatccgcc acgcgggtat atcgttccag
     2461 gcataccata tgggcggcaa acgccgcagc gctccccaga gtcagcagat ccccaaggtt
     2521 ggcggaggcc gtcggcctga ccagcagcca gagaccagcc aatgcaatcc cattggagac
     2581 ccaggtgtgg aggcccagac ggagcaggta caatggcaca aacaccacat acagcaccgt
     2641 gataaaggcc gaattcgatg ccgtcgtgaa gcgaagtccc acggtctgca acacatagcc
     2701 gatgaacagc caggcggtcg caatcagact ggcccgcatc atggaggtgt cacgaatcaa
     2761 agggcggcgg aagaacagca acacggcaaa gacgacggtc atgccgagga ggaatcgcag
     2821 aaaaagaaac gatagcgggg agatttgttc gagcgcggcc ttcgtggctg gaaacgtcgc
     2881 cccccacaca aaggtcgtga gaagcagcgc gaattgaggc atggcaaaca gaaatcacga
     2941 acggtgaatg ctgtacgtgg gatcggagga accgcctccg gaaatcacgc cgtcatcatt
     3001 caacattcat ggttcatcat tcctggcgtt atggtttgca caacgcgcat ttacgctctt
     3061 cgctggtgcc cgcctgttcc atcgcggcga gcgcccgctc cttatcgggg tagtcgaacc
     3121 atcccccttg ccagtagtcg ctgctcgaca cgccggaagg gacttcggag caccggtccc
     3181 gatggagcgt cacgcgatcg atcgcccgtg caatatgaag ccaatacacc ataatacccc
     3241 gtccaattaa tacgtcatga ccgtcacggc tcaacctacc acgtgccccg ataggagagc
     3301 aatgcgcagc agacaccgag ccgagacgag tctcgtgacg gcgtggaaca ttcgattatg
     3361 ggatgaaacc gggaggtcgc gctagggcag gagctgccac tcgtccttct cgatcacggt
     3421 cttgacgccg gttcccagct tcttgacgtc tttctcttcg aagagacgca tgtagcgttc
     3481 aatccggtcc ggatcgtcga tacgctcttc ctgcatcaca ccaccgctgc ctttgtactt
     3541 ccgaattaac aggggcattt caaccaatcc tccaattgtg tctcgcgttt cggcacaaag
     3601 tttcggtaca atagtgcaaa gctacgcaca gggtcaagcg tttccaacgg gagaatcgct
     3661 ttatccagcc ttgcccctca gggcgaggcc aggaggcgcc atccaaggag tcggctatgc
     3721 cggtctacga atatcgctgc gaacaatgta cccatcagtt cgaggcaacc cagtccgtcc
     3781 acgcacgacc ggaagacacc gtctgtcccc agtgcaacaa aatcggcgcc accagaatgc
     3841 tctcggcgtt tgcttcgaag gtcaaaggct cccacaagcc gggcttcgaa gagatcaaag
     3901 cctataacat gttgaacgag cgtatggatc ggttttccaa gctcccgccc gccatgggca
     3961 aacggatcga ggcctcaccg gacttcatgg ccggcgccgg gggccctgct ccaactgaag
     4021 ggcacgactc gtaggcgaag ccgtgttacc gggcgggcgc aacaggctca cgtagaacac
     4081 cgccggagag agccgatcag cggctcaagg ctgacaggag actcgttcag catgcacacc
     4141 cgtatcagcg gatttgtctg tgccgctctt ctctgtctca cgggcctccc ggcttcagcc
     4201 gaggtggccg gatcaatggc catcgccggc cacgggcccg aacagcgggt catcgaatcg
     4261 ctggcccatg cgtttgaaaa agccaatcct cgcgcctaca tcgacgtcgt ctgggacgac
     4321 aattcgaagc cgctcgatct ggtgaagacc aaacaggcca aaattgcggt gaccggaacc
     4381 gcggaggacg gcctgcgctc gttccagatc gcctgggacg gcatcgccat catggtgcac
     4441 cggtcgaact tcaccaaaga ggtcacaaaa caggaagttg ccgagctgtt ttccggaaag
     4501 tacaaagtat gggccgatct cggcggaccg gatacgaagg tccttctgat cgaccggccg
     4561 cgcaacgaaa acaatcgcga cgccttcgag caacagctcg gcatcgccgg caaaattccc
     4621 gagggcgcaa agatcatcgc gaaagacgaa aaagtgatca agaccatcgc cggcaccctt
     4681 ccaccccact cggccgtctc ctatgtgtct ctcggccagg ccctggaggc agtggcatcc
     4741 ggtgtgcccg tcaagctgct gccggtcgat aagattgaac cggaaacacc taccgtcaag
     4801 gatggccggt acaccctgcg ccgcccgatc ctgttgctgt cgcacaatga gcccgatccg
     4861 ctcgttgacg ccttcgagca atttgccctg tcggaggacg gacaaaaaat catcagcgag
     4921 tcttatacgc cgctcccgaa gacatcatct ccatgagagg aggcttttat gcgcactatt
     4981 gtcacctgca tgctcggctg cacccttgtg ctcggaacca cgttcgttgc cgtccccgct
     5041 gggcatgccc aggaaggagc gatggtcgag ggcgccgggt atacgatgtt caataccgag
     5101 tcgatcaagg gatcggacac ctccaaactg gagagcgacc cggtctgtga ccgttccaaa
     5161 cgtccttcca ttgcgaaagt tgaaccggat gaagcgaaac ccggtgaaaa aatcaccatc
     5221 aagggtgaaa atttcgggag caaggaatgc ttccatggcg tgaccttcag cgccgcctcc
     5281 aaggaaaaga tcgactaccg tttcgtgaac gagactacga tcgaggccgt agtgcctgag
     5341 gcgaaagccg gcatgtcctt tatcatcgtg gtggccggcg gaggcagcgc ccaatcgaag
     5401 cccctcctca ttcaacccaa gtaagagatt catcaggcag gaggtgcctc acctcctgcc
     5461 cgtttccgcc cctcccacgg tccatccttt cgtcccggtt cgtctcgaca actctcttga
     5521 ccctatgcta ggttacgcga tctttttgga ggcggatcag aggtgagcta cggctccaag
     5581 cctctcgcaa tcccatcatg tttcggaaga tccttattgc caaccgtggc gaaatcgcca
     5641 tgcgcatcat ccgtggctgt cgtgagctca atatcgcgac agcggcaatc tattctgaag
     5701 ccgactcctc cgggatttac gtcaaaaaag ccgacgagtc ctacctcgta ggcccgggcc
     5761 ccgtcaaagg tttcctggac ggaaagcaga tcgtggaaat cgccaagcgc atcggcgccg
     5821 acgcaatcca tcccggatac ggattcctct ctgaaaacac caaattcgcc cggctctgcc
     5881 aaacctccgg catcaccttt atcggtccgt ctcccgagac gatcgacctc atgggcagca
     5941 aagtaaaggc gcgacagatc gcccaacagg caggcgtccc gatcgtcccc ggcaccgaag
     6001 gcggagtcac cagcgtcgac gaagccctgg ccttcgccca tcagatcaac tacccggtca
     6061 tgatcaaggc cagcgccggc ggcgggggac gaggcctgcg tgtggttcgc tccgatcagg
     6121 aattgcggga gaacattgac gtcgcgtcgc gagaagctca ggccgcgttc ggcgacggca
     6181 gcatcttcat cgagaaatac atcgagcggc cgcaccatat cgaatttcaa atcctgggcg
     6241 acaaacacgg caacatcatc cacctgggcg agcgggattg ttccattcag cgtcggcacc
     6301 agaaactgat cgaaatcgcc ccgtcattga tcctgacacc caaactacgc gcccaaatgg
     6361 gcgaggccgc cattgccatc gcgaaagcgg tgcactacga caatgccggc accgtcgagt
     6421 ttctcctcga ccatgagggc catttctatt tcatggaaat gaatccccgt ctccaggtgg
     6481 aacataccgt cacggaacag atcacggcca tcgacatcgt ccggaatcaa atttccattg
     6541 cggcggggaa gccgttggag atccggcaaa aggatgtgac cctgcagggc catgcgattc
     6601 agtgccgcat caatgccgaa gacccccgca acaacttcat gccctgcaca ggcaccatca
     6661 ccgcctatct gtcacccggc gggatcgggg tccgcatcga cggcgcggtt tatcgggatt
     6721 acacgattcc tccctactac gatgcgctgc tggcgaaact caccgtccgc gggcgcacct
     6781 gggaagaggc cgtcagccgc atgcggcgct cgctggaaga atatgtcctg cgcggggtga
     6841 agacgaccat tccgttcatg aagaacgtga tgatggaaca ggattttcaa gccggacgat
     6901 tcgatacgtc ctacctggaa acccatccgg acttgtatca atacgaagaa tccgaggagc
     6961 ctgaagacct ggtgctggcg atctccgcag cgatcgccgc gtacgaagga ctctgacctc
     7021 cccagccact cggcaccaac acctcaaccc tgttacgcga gcggattcgc cgccgaaggc
     7081 cgccagtatg gcaacgaaaa agaccaagaa aaccgccccg aagaaaagcc ccgcgaagaa
     7141 gaacgtgcct gcaaaaccca gccggcccgc ggcctcacgc gccgtccagg ttacgcagcc
     7201 ggcgagccct gagttccgcg tgaccccggc gccggggaaa aagctgttga tgaccgaggt
     7261 tgcgttgcgc gacgggcatc aatg
//
LOCUS       DAORRQ010000122         7170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_408_length_7170_cov_92.998173, whole genome shotgun sequence.
ACCESSION   DAORRQ010000122 DAORRQ010000000
VERSION     DAORRQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_408_length_7170_cov_92.998173"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..1816)
                     /locus_tag="PK224_19990"
     CDS             complement(269..1816)
                     /locus_tag="PK224_19990"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRB18086.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKLMIAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIALEVEGPVVCSLARARPEDITRASEALRGSPRVRIHTFLST
                     SDIHLMHQFRMTREEAKKRAVEMVQLARTYVDDVEFSPMDASRSDPAYLCEVIEAVIA
                     AGAGTINIPDTVGYAVPQEFGGLIKRIKDSVPNSGQAVISVHCHNDLGLAVANSLAAV
                     VAGAGQVECTINGIGERAGNTSLEEIVMGLRTRRDWYGADTKVVTEEISKTSRLVSKI
                     TGMVIQPNKAIVGANAFAHTSGIHQDGLLKDKTTYEIMRPESVGLEQVKLVMGKLSGR
                     HAFRQRLEDLGYKLTEEEVNHAFERFKRLADQKKEIYEEDLEVIVSEEVAKMSERVVL
                     KSFQVESGTNKTPTATVELEIDGKLVSHSGTGDGPVDAVYRTIAAMTQTKSKLLMFGV
                     NAITGGTDAQGEVSVRVEEDGRTVSGHGADTDIITAAARAYLNALNKLAYFAAKQAEG
                     IQKVSLI"
     gene            complement(2234..3019)
                     /gene="pssA"
                     /locus_tag="PK224_19995"
     CDS             complement(2234..3019)
                     /gene="pssA"
                     /locus_tag="PK224_19995"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRB18087.1"
                     /translation="MRAPFAKGNRKRQAMYLIPNLCTTGNLFCGVFAILSVFNGQHLV
                     AAIAILVGMIFDMLDGKLARLTNSTGQFGIEYDSLSDVVSFGVAPGVLIYSYALSGQG
                     MFGVAVMFAYVAMGAVRLARFNATVSTSDSKYFTGLAIPAAAGVIASLVIFDLHITQM
                     GSEVKPLLILVITFALAFLMVSTIKYRSFKDMKFRRGDHFTYLVWGILALMLIVAWPQ
                     AMLFVTFTGYAVSGPLARLWTMVSKSAGKPVAKTDTPVLDSRE"
     gene            complement(3031..3681)
                     /locus_tag="PK224_20000"
     CDS             complement(3031..3681)
                     /locus_tag="PK224_20000"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRB18088.1"
                     /translation="MADRAVGIPIVKEGLPFVGGLVGATLLFGAAGWIIPAVIAGGFT
                     LFTAWFFRNPSRTIPQQPNVVVSSGDGKVIAIEEEFEPRYLKEKSIRVTVFLNVFDVH
                     VNRMPCAGTVEGVSYQPGQFLVASKPEATLRNEQNAVMLKTESGAKVLCVQVAGLIAR
                     RIVCWVGQGDRVERGERFGLIRFGSRMDTFLPLGSKICVSLGDRVKGGETIVGELR"
     gene            complement(3733..4746)
                     /gene="ilvC"
                     /locus_tag="PK224_20005"
     CDS             complement(3733..4746)
                     /gene="ilvC"
                     /locus_tag="PK224_20005"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRB18089.1"
                     /translation="MKIYYDKDADLQLIRGKKVAVIGYGSQGHAHSLNLKESGANVVV
                     GLREGSSWKKAEASGLKVMPVADAVKASDVIMILAPDEAQAAIYRQEIAPNLKPGSYL
                     AFGHGFNIHFGQIVPPADINVFMVAPKGPGHLVRSEYTKGSGVPCLLALHQDPSGNTR
                     QVGLAYASAIGGGRAGIIETNFREETETDLFGEQAVLCGGLTSLIQAGFETLVEAGYS
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDITRGPRVVTDQTKQEMKKI
                     LGEIQSGQFAKEWVLENQANRPVYNALLKKGEGHPIEEVGARLRSMMPWLKKDQLVDK
                     NKN"
     gene            complement(4814..5332)
                     /gene="ilvN"
                     /locus_tag="PK224_20010"
     CDS             complement(4814..5332)
                     /gene="ilvN"
                     /locus_tag="PK224_20010"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRB18090.1"
                     /translation="MEHIISVTVENKFGVLSRVAGLFSGRGFNIESLSVAPTLDPSMS
                     QMTIVTSGDERIVEQIVKQLNKLIDVIKVVDLNESEFVSRETALIKVHTKAEDRAEAL
                     RIADIFRANVIDSTPSTYTIEVTGDPKKLEAIINLLQPLGIKELIRTGRVAIAREAIR
                     PALSQPKKIARE"
     gene            complement(5374..>7170)
                     /gene="ilvB"
                     /locus_tag="PK224_20015"
     CDS             complement(5374..>7170)
                     /gene="ilvB"
                     /locus_tag="PK224_20015"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRB18091.1"
                     /translation="CEGEGDMKLTGSEILIECLKAEGVKTIFALPGGVVLKIFDMLHQ
                     QKDIEVVLTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLV
                     CISGQVSTSLIGNDAFQEADNIGLSRPCTKYNFLVKDVNDLATTIKEAFYIATTGRPG
                     PVLVDIPKDVSMAKTEFTYPSSVSIRGYNPTYEGNKWQIKQAAEAIMKAKKPILYVGG
                     GVVFSQASKELLELAEMTQIPVDMTLMGLGAFPGEHPQSMGMMGMHGTYCANMAVHYS
                     DLVIAVGARFDDRVTGKVSEFCPFAKVIHIDIDPTSIRKNIHVDIPIVGDCKAVLREL
                     NQILRATVNGNQKDLRKPWWDQIREWQQAHPLAYQQEPDGAIKPQHVVKRLYELTKDR
                     DPIVATDVGQHQMWTAQYFKLARPNRWLTSGGLGTMGFGFPAAMGAQAAFRDRLVLCV
                     AGDGSIQMNMQEMAAAVVSKLPVKVIILNNRFHGMVRQWQDLFYEGRYASSYLDTTPD
                     FVKLAEAYGAVGLRASKVGDLDTVLKEAIATDKPVVVDVPTYPYENCYPMIPAGGCNH
                     EMILEDPPELKRRMAGAPSTGSDEDKDTILTA"
BASE COUNT         1500 a         2287 c         1835 g         1548 t
ORIGIN      
        1 aagattttaa gcgatgccag aggctctgct gacccggggc gagcagtccg tgcgccaggc
       61 tatatccgag cgcgagcgat gcctttgccg ggtgcagcaa ttcccgcagc ctggtgtgat
      121 cgcccgccaa gaattccggg catcgttgaa gatgcgtgtt tagcatcgtg caccgagatc
      181 catagtagga atgacagacg atgtcgctcc gtccagtccg atacgaacgc atcggcccgg
      241 acggaggaat ctaccctgca ggttgggctc aaatcaagct gaccttctga atcccttcag
      301 cctgtttcgc agcaaaatag gccaatttat tgagcgcatt cagataggcc cgcgcagccg
      361 ccgtaatgat atcggtatcg gcgccatggc cggagaccgt tcgaccgtcc tcttcgaccc
      421 gcaccgacac ctccccttgc gcatccgtcc cgccggtaat ggcattgacc ccgaacatca
      481 gtaacttgct cttcgtctgg gtcattgcgg cgatcgtccg gtagaccgca tccaccgggc
      541 cgtcgccggt tccgctgtga gagaccagtt tcccatcgat ttccaattcc acagtcgccg
      601 taggagtctt attggttcca ctttcgactt gaaacgattt caataccacc cgttccgaca
      661 tcttcgcgac ttcttcggac acaatgactt cgaggtcttc ctcataaatc tctttctttt
      721 gatccgccag ccgcttgaat cgttcaaacg cgtggttgac ctcttcttcc gtcaacttat
      781 agcccaaatc ctcgagccgc tgacgaaacg catggcgccc ggacaacttg cccatcacca
      841 gcttgacctg ctcaagaccg acggattcag gccgcatgat ctcgtaggtc gtcttatctt
      901 tcagcaaccc atcctggtga atccccgacg tgtgggcgaa ggcattggca ccgacaatgg
      961 ccttgttcgg ttggataacc ataccggtaa tcttgctcac caggcggctg gtttttgaaa
     1021 tctcctccgt cacgaccttg gtgtcagccc cgtaccaatc tctacgcgtc cggagaccca
     1081 tcacaatctc ctccagcgag gtgttgccgg cccgctcgcc aatgccgttg atcgtgcact
     1141 cgacctgtcc ggcccctgcc accactgccg ccaagctgtt agcgacggcc agccccaaat
     1201 cgttgtggca atggacggag atgacggctt gcccgctatt cgggacactg tctttgatcc
     1261 gcttgatcag gccgccgaac tcttgaggca cggcataccc caccgtgtcg ggaatattga
     1321 tcgttcccgc cccggccgca atgaccgcct cgatcacttc gcacagatat gccggatctg
     1381 accggctggc gtccatcggt gaaaactcga cgtcatccac ataggtgcga gccaactgca
     1441 ccatctccac cgcgcgcttc ttcgcttcct cgcgggtcat ccggaactgg tgcatgaggt
     1501 ggatgtccga cgtcgagaga aacgtatgga ttcgcacacg gggcgagccc ctcaacgctt
     1561 ccgacgccct cgtgatgtct tccggcctgg cccgcgccag gctgcacacc actgggcctt
     1621 cgacttccaa agcgatccgt ctgaccgctt cgaaatctcc gggtgaactg taggcgaagc
     1681 cggcttcgat gatatccacg ccgaggcgcg ccagctgctt ggcgatcata agtttctctt
     1741 cgacgttcat gctcgccccc ggtgactgct ccccatcccg caacgtcgta tcgaatattc
     1801 tgatcatcct ggtcatggct taccctcctc ctgtcccgca gcttgctcaa acggatcttc
     1861 caacgcggcc gcaggcgaga aaacaccgga ggcgtaccct ctggggtacg ttgaggatgt
     1921 tttcgagccg agaacaaagt tggaaggccg tttcagcatg ctgccaataa aaaaagcctc
     1981 cgccccggga cagggacgaa ggccttgcga gcttcgtggt accaccctga ttcagcgagg
     2041 gatgaagtat gtccttcatc cgaacgccct tcattcaatc cgtgacgcgg atcattcgga
     2101 acccattaca accctggggg gcttcacaag tccagctcag aggcgagttc ggcagatcac
     2161 cggctggttc gcaccatcca ccagctctct cagttcggtc agcgctgcct actactcctc
     2221 gtcgtcgctg ttgttactct ctcgaatcga gtaccggcgt gtccgttttc gccaccggct
     2281 tccctgcact cttgctcacc atcgtccaca gccgcgccaa tggaccggac acggcgtacc
     2341 cggtaaacgt cacgaacaac atcgcctggg gccaggcgac aatcagcatc aaggcgagaa
     2401 ttccccacac cagataggtg aaatgatccc cacgccggaa cttcatatcc ttgaagctgc
     2461 gatacttgat cgtactgacc atcaagaacg ccagcgcgaa cgtgatcacg aggatcaaca
     2521 gcggcttcac ttccgagccc atctgggtga tgtgcagatc gaagatcacc aacgacgcaa
     2581 tgacaccggc tgcggcagga atagccaagc ccgtgaagta tttgctgtcg gaggtgctca
     2641 ccgtcgcatt gaaccgggcc aggcgaaccg cccccatggc cacataggca aacatcactg
     2701 cgaccccgaa catgccctgg ccgctcagcg cataggaata aatcagcacg ccaggtgcga
     2761 ccccgaacga caccacgtcg gaaagagaat catactcgat gccgaactgg ccggtactgt
     2821 tcgtcagtcg ggccaacttc ccgtccaaca tgtcgaaaat catgccgacc agaatggcga
     2881 tggctgccac cagatgctgc ccgttaaaca ccgacaggat cgcgaacacg ccgcagaaca
     2941 gatttcccgt cgtgcagagg ttcgggatca gatacatggc ctgtcgcttt cgattaccct
     3001 tcgcaaacgg agctcgcatg gccggtgatt tcatcggagt tcccccacga tggtttcgcc
     3061 ccctttgacg cggtcaccca aggagacaca aatcttcgag ccgagcggaa ggaaggtatc
     3121 catccgcgac ccaaaccgta tcagcccaaa ccgctctccc cgctccacac gatcaccctg
     3181 cccgacccag cacacgatcc ggcgggcaat cagcccggca acctgcacac atagcacttt
     3241 cgcccctgac tccgtcttca acatcacggc gttctgttcg tttctgagcg tcgcctccgg
     3301 cttgctcgcc acgagaaact ggccgggctg gtaactcaca ccctccaccg tcccggcgca
     3361 gggcatccgg ttcacatgca catcgaagac gttcaagaac accgtcacac gaatgctctt
     3421 ttccttcagg tagcgcggct cgaactcttc ctcaatcgca atgactttgc catcccccga
     3481 cgacaccacc acattcggct gctgcggaat ggtccgactc gggttccgaa agaaccacgc
     3541 cgtaaacagc gtgaacccgc ccgcaatcac ggccggaatg atccaaccgg ccgctccgaa
     3601 cagcagcgtc gcgcccacaa gaccaccgac aaacggcaag ccttctttta cgatcggtat
     3661 cccgacggct cgatcagcca tagacctcca ggccttctta atgatgctat cacgaagagc
     3721 ccggtcgcag ggttagtttt tgttcttatc gacgagctga tcttttttca accagggcat
     3781 catcgagcga agtcgggcgc cgacttcttc aatcggatgg ccctcgccct tttttagcag
     3841 ggcgttgtag accggccggt tcgcctggtt ctccaacacc cattctttcg cgaactgacc
     3901 gctctggatc tcgccgagaa tcttcttcat ttcctgcttg gtctgatccg tcaccacgcg
     3961 cggtccgcgg gtgatgtcgc catacttcgc cgtggtgctg atcgaatagc gcatgttggc
     4021 gatgccgccc tgatagatca ggtccacgat taatttcact tcatgcagac actcgaaata
     4081 ggccatctca ggcgaatacc cggcctcgac gagcgtctcg aacccggcct ggatcaggga
     4141 ggtcaaccct ccgcacaaca ccgcctgctc gccgaacaaa tccgtttcgg tttcttcacg
     4201 gaagttcgtt tcaatgatgc cggcccgtcc gccgccgatg gcgctggcgt aggccaaacc
     4261 cacctggcgc gtgttgccgc tgggatcttg atgcaatgcc agcaaacaag ggacgccgct
     4321 gccttttgta tattccgacc gcaccaggtg acccggtccc ttgggcgcca ccatgaagac
     4381 gttgatgtcc gcgggcggca caatctggcc gaagtgaata ttgaacccgt gaccgaaggc
     4441 caaataggac ccgggcttca gattcggagc gatctcctgg cggtaaatgg ctgcctgtgc
     4501 ctcatcggga gccagaatca tgatgacatc ggaggctttg acggcatcgg ccaccggcat
     4561 gaccttcagt ccgctcgctt ccgccttctt ccaggaactg ccttcacgca aacccacgac
     4621 gacattggcg ccgctttcct tgagattgag cgaatgggca tggccctggc tgccatagcc
     4681 gatcacggcc actttctttc cgcgaatgag ctgtaaatcg gcatctttgt cgtaatagat
     4741 cttcatacat cactcctgaa ggttcatagg gcacatgggt ggaaacgaca gttgcacaca
     4801 ggggatgagc gaattattcc cgggcgatct ttttgggttg gctgagggcc ggccggatcg
     4861 cttcacgcgc aatcgccact cgccccgttc gaatcagttc cttgatgccg agcggctgga
     4921 gcagattaat gatggcctcg agcttctttg gatcacccgt cacctcgatg gtgtaggtcg
     4981 agggcgtcga gtcaatcacg ttggcccgga aaatatctgc gattcggagc gcctcggctc
     5041 ggtcttctgc cttggtatga accttgatga gcgccgtctc ccgcgaaacg aattcactct
     5101 cattgagatc cacgaccttg atgacatcga tcagcttatt cagctgcttg acgatttgtt
     5161 ccacgatccg ttcgtccccc gacgtgacga tggtcatctg cgacatcgag ggatccagcg
     5221 tgggggcgac cgacaaactt tcgatattga agccgcggcc gctgaacaac ccggccacgc
     5281 gggataacac gccgaactta ttttctaccg tgaccgaaat gatatgttcc atcggaaaac
     5341 tctatcctga tagctgtcgg ctgacagcgt gatttaagcg gttaagatcg tgtccttatc
     5401 ttcgtcggac ccggtgctgg gcgcgccggc cattcttcgc ttcagttcag gcggatcttc
     5461 gagaatcatc tcgtgattgc acccgcccgc cggaatcatc ggataacaat tctcataggg
     5521 ataggtcggc acatcgacga cgacgggctt gtcggtcgcg atggcttcct tcaacacggt
     5581 atccagatcc ccgaccttgc ttgcgcgcaa accgaccgcc ccataggctt cggccagctt
     5641 cacgaaatcc ggcgtggtgt cgaggtagct cgacgcatac cgtccttcgt aaaacaggtc
     5701 ctgccactgc cgaaccatgc cgtgaaaacg attgttcaga ataatcacct tgaccggcag
     5761 cttcgacacc accgcggcgg ccatttcctg catattcatt tgtatgctgc cgtcgccggc
     5821 cacacagagc accaatcgat ctctgaacgc ggcctgcgcc cccatagcag ccggaaaccc
     5881 gaaccccatg gtacccaaac cgcccgaggt caaccagcga ttcggtcggg caagcttgaa
     5941 gtactgcgcc gtccacatct ggtgctggcc cacatcggtc gccacgatcg gatcacgatc
     6001 cttcgtcagt tcatacaacc gcttgacgac atgttgcggt ttaatcgccc catcgggctc
     6061 ctgctggtag gccaacggat gcgcctgctg ccattcgcgg atttgatccc accagggctt
     6121 gcgcagatcc ttttggttcc cgttcacggt ggcacggaga atttgattca gctcccgtaa
     6181 gacggccttg caatcgccca cgatgggaat atcgacatgg atattcttcc ggatagaggt
     6241 cggatcgata tcgatgtgaa tgaccttcgc gaacgggcag aactccgaca ccttccccgt
     6301 cacacgatcg tcaaagcggg cccccaccgc gatgaccaga tcggaataat ggacagccat
     6361 gttggcgcag taggtaccgt gcatgcccat catgcccatg gactgcggat gttcgcccgg
     6421 gaacgccccg aggcccatca aggtcatatc cacggggatc tgagtcatct ccgccaattc
     6481 gagcaactct ttggatgcct gcgaaaagac gacgccccca ccgacataca gaatgggctt
     6541 cttcgccttc atgatggctt cggcagcctg cttaatctgc cacttatttc cctcgtaggt
     6601 cggattgtag ccgcggattg agaccgagct gggataggtg aactctgtct tggccatgga
     6661 cacgtcttta ggaatatcca ccaagaccgg tcctggacgg ccggtcgtgg cgatatagaa
     6721 cgcttccttg atggtcgtcg ccaggtcgtt cacgtccttc acgaggaaat tgtattttgt
     6781 gcaaggccgg ctaaggccga tgttgtcggc ttcctgaaag gcgtcgttgc cgatcaaact
     6841 cgtcgagacc tgcccgctga tacacaccaa cggcacggaa tccatatagg catcggccag
     6901 agccgtaatc acgttggtca taccaggccc ggacgtcacc aaacacacac cggccttgcc
     6961 ggtagccttg gcatagcctt cggccatatg ccccgcgccc tgttcgtgcc gggtcaagac
     7021 gacttcgata tctttttgct ggtgcagcat gtcgaaaatc ttcaacacca caccgcccgg
     7081 caaggcgaaa attgttttca cgccttcggc ttttaagcat tcgatcagga tttccgaccc
     7141 tgtaagcttc atatcaccct ccccttcgca
//
LOCUS       DAORRQ010000123         7095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_414_length_7095_cov_269.669602, whole genome shotgun sequence.
ACCESSION   DAORRQ010000123 DAORRQ010000000
VERSION     DAORRQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_414_length_7095_cov_269.669602"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..2112
                     /gene="pilQ"
                     /locus_tag="PK224_20020"
     CDS             118..2112
                     /gene="pilQ"
                     /locus_tag="PK224_20020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HRB18092.1"
                     /translation="MKHAQVGVHPLLSVTVMAGVLGLAGYVQAAAPGETTGLTGFTQA
                     TDQSAALSVESAAPVPERAVAAGSAATMLTKVEAKPDGSRMALILSGNGVFTHTVKMI
                     GDRRMVVDMPHIQSDGHQSQLMVGHALLSRVRFGYHPDKVRLVLDLSQSVEHTIDSTD
                     GRLVLTLEPKAVASVTETKAAAGSAMAQIEASAEQAVSLEPKQSTSHPTLVAHKSPKA
                     LFRIQPIQLTAESVQKEDRKPENNEVVNGASRFVGRRISLDFQQADISNVLRLIAEVS
                     GFNIVVGEGVKSKVTMKLANVPWDQALEMLLKMNALGMIRQGNIVWVDTLQNIAKQQD
                     EEARAKDSKAKAEPIVTRVFYIRNLNATEVQTSLRQNLSPRGTMTVSAASNALIISDT
                     ESKLEVLRQLLDGVDLEVPQVQIEARIVQADTTYTRSLGVQWGIQNVNQLGSSSGTSA
                     FKTGTTGAFGAQVSDFLINLPANPGLPSVPGAGFSIGKTDGAMLDVRLSAGELLGLTK
                     VIAAPKITTLDKRDAKIAQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEI
                     GKQILMKVRATRNAVGARSNPAGPSIDRREATTQVLVRDGETMVIGGVFVDTQSNNVA
                     GIPYLSRIPVLGWLFKNKTENVSKQELLIFLTPTIVRSTT"
     gene            2385..3542
                     /gene="aroB"
                     /locus_tag="PK224_20025"
     CDS             2385..3542
                     /gene="aroB"
                     /locus_tag="PK224_20025"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRB18093.1"
                     /translation="MTRSSIDRPSHSIHVELGARSYAIVIRRDLLREIGEELKRLKCA
                     GKVGVVTDRNLAKHYLTQVTRRLKAAGFTVVPVVLPPGERTKTLRSIGTVMDALVNAR
                     FERSSTLLALGGGVIGDITGFAAAIYQRGIPFVQVPTSLVAQVDSSVGGKTGVDHPKG
                     KNLIGAFNQPRAVFIDPAMLATLPPREWVAGLAEVIKYGVIADAAFFEYLEQNMESIQ
                     KLTHDAVAHIVKRSCEIKAQVVSEDEREADRRRILNFGHTIGHALESLGGYRGFIHGE
                     AVAVGMVYEADLARHLGYCGAEVVARLRALVKAAGLPDRLPKVAFNALWGAMQQDKKV
                     SAGTIFCVVPEQIGAVRIVPLEKATTRAWFQDVHTREAAELKRARPVGRAR"
     gene            3550..4809
                     /locus_tag="PK224_20030"
     CDS             3550..4809
                     /locus_tag="PK224_20030"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HRB18094.1"
                     /translation="MATKRTLTQVEQALREKTREVDVLHRISESISNTLDLEAVLRHI
                     VDMVVEVTKADACLLYLLSDNRDELILRASKNPHPKLIGRITIGMGEGITGWVARERT
                     RVVIPNSASDDTRFKFFHNLPEDRYQAFVSVPITTKQEVVGVINVQHKRSRRYRPDEL
                     ALLSTIATQVGGAIENARLYDQMTRKALQLDTLSQVSETVSSNRLIEDVLQLIVTMTA
                     QMMGSKICSIILLDQPTGELRIAATQSLSEQYRRKPNVKIGQSISGRAVQDRRPIIVP
                     DVIKEGTYMYPDMAAKEGLCSLLCVPMLVREKAIGVINTYTSKPHTFTAEDVKLMQAI
                     ANQAAISIEHTTLLEKSFEMQEALQVRKLLDRAKGYLMRSKRLSEEDAFKLIQRQSMD
                     LRKSMREIAEAILLAGEIEERADKGRS"
     gene            5107..5496
                     /locus_tag="PK224_20035"
     CDS             5107..5496
                     /locus_tag="PK224_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18095.1"
                     /translation="MAKSRRVATAFLLTLMTSACATGQYTPLVGADRDEVEQLKENVY
                     RVEYRVSSFTSQEQLDAYLRRRCAELTLREGYDFFHLAQRADVLALSRRTSMTVTMYK
                     GKKPVGADDVYDAKEVLAQPDLLSGSQ"
     gene            complement(5513..6415)
                     /locus_tag="PK224_20040"
     CDS             complement(5513..6415)
                     /locus_tag="PK224_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18096.1"
                     /translation="MIRRPTPTQSRLSSFRLAWQLALPCLLLCACSLGTNLTPVGQGP
                     LGAVALERQTSRGTTAKYGTPQTFQASHPAHLTASLTGRLLTGLSVSGLDRPGSSIAQ
                     ESYPLFSHEEVEFLAPLIVGALAQAQPDQRVRFTMQDDGLTTQGTLYVYKTTLRVNLS
                     HYRSASGDGQTKPASLILSFTTPQALVRADVPQPWMTVEPEQPRIAVSLEALSQLPAP
                     SPVAADHNTTAEAAPTQTSPTAEQSRLQQELQSTKDVVVKQAEELQKLKAELESVRRQ
                     LAEKDVAASKPKPKAAPRKPTATP"
     gene            6744..>7095
                     /locus_tag="PK224_20045"
     CDS             6744..>7095
                     /locus_tag="PK224_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRB18097.1"
                     /translation="MRSLRIAMAQINPTVGDIVGNTRLIQTWIKEARQAKADLVAFPE
                     LAITGYPPEDLLFKPRFIEDTHRALKAVAAEARGVVVVVGYVGQGATAVSASDASSFP
                     LSGRHELYNEAAVLS"
BASE COUNT         1436 a         1917 c         2274 g         1468 t
ORIGIN      
        1 tgtggtcagt tcaatcaccg aacgaggcat catcgttcaa gagcggttca cggacgtgta
       61 cgggaataaa caggagcggg aatacgtcaa gctcctgcac ccgaaagagg gtacagaatg
      121 aaacatgcac aggttggtgt acatccgctg ctgagtgtca ctgtgatggc cggagtgttg
      181 gggcttgccg gatatgtgca agccgcggcg ccaggcgaga cgaccggact gaccggattc
      241 acgcaggcca ctgaccagag tgcggccctg tccgtggaga gcgccgcacc cgtccctgag
      301 cgtgcagttg ccgccggctc ggctgcgacg atgctgacaa aagtggaagc gaagcctgat
      361 gggagtcgga tggcgttgat tctgagcggg aacggcgtct tcacgcatac cgtgaagatg
      421 atcggggatc gtcggatggt cgtcgatatg ccgcatattc agtcggacgg ccaccagtca
      481 cagctgatgg tcggtcatgc cttgttgtcg agagtccgat tcgggtatca cccggacaag
      541 gtgcggttag tgctggattt atcgcagtcc gtcgaacata ccatcgattc aaccgacggc
      601 cgcctggtcc tcacgctgga gccgaaggcg gtggcgtccg tcactgaaac gaaggctgcg
      661 gccggtagcg ccatggccca gattgaggcg tcggcggaac aggctgtctc gctggagccg
      721 aagcaatcga cgtcccatcc cacgttggtg gcgcataaat caccgaaagc tttgtttcgt
      781 atccagccga ttcaattgac ggcggagtcg gttcagaaag aagaccggaa gccggaaaac
      841 aatgaggtgg tgaacggcgc ctcacgattc gtcggtcgtc gtatttccct cgattttcag
      901 caagccgata tcagcaatgt gctgcggttg attgcggagg tcagcggctt caacattgtg
      961 gtcggggagg gcgtcaaaag caaagtcacg atgaagctgg cgaatgtgcc gtgggatcag
     1021 gccctcgaaa tgttgctgaa aatgaatgcg ttggggatga ttcgccaggg caacatcgtg
     1081 tgggtggata ccctgcagaa tattgccaag cagcaggatg aagaagcgcg ggcaaaggac
     1141 tcgaaggcga aggcagagcc gatcgtcacc cgggtattct atatccggaa tctcaacgcc
     1201 acggaagtgc aaacgtcgtt acgccagaac ttgagtccgc gcgggaccat gacggtcagc
     1261 gccgcgagca atgccctcat catcagcgac acggagtcca agctggaagt cctgcggcag
     1321 ttgctggacg gcgtcgatct cgaagtaccg caagtgcaga ttgaggcccg catcgttcaa
     1381 gccgacacga cctatacccg ctcactcggt gtgcagtggg gtatccagaa cgtcaaccag
     1441 ctgggaagct ccagcggaac gtctgccttc aagaccggaa ccaccggggc attcggcgcc
     1501 caagtctcgg acttcctgat caacttgccg gctaaccccg gattgccgtc ggtgccgggt
     1561 gccggattct cgatcgggaa aaccgacggg gcgatgttgg atgtgcggtt gtcggccggt
     1621 gaattgctgg gcttgaccaa agtgattgcg gcgccgaaga tcacgacgct ggacaagcgg
     1681 gacgccaaga ttgcccaggg tgaatcgatc ccattccaga ccacgtcctt gcaggggacc
     1741 cagacgacct tcgtggatgc gaacctggag ttgaacgtga cgccgcaaat tacctctcgc
     1801 gacccgaaag agatcggcaa gcagattctc atgaaggtgc gggccacccg gaacgccgtc
     1861 ggcgcccgga gcaacccggc cggtccgagc atcgatcgtc gtgaagcgac gacccaggtg
     1921 ctggtgcgtg acggggaaac catggtcatc ggcggtgtct tcgtcgatac tcagtcgaac
     1981 aacgtggcag gaattcccta tctgtcacgt atcccggtcc tgggctggtt gttcaagaac
     2041 aagaccgaga acgtctccaa gcaagagttg ctgatcttcc tgacgccgac gatcgtgcgc
     2101 tctacaactt gacaggtccc agcgcggcaa gttaataatc gcccccattg aagatggtac
     2161 gctatctcaa tgggggcgaa tcctgcggaa cgtgtcttct ccccctatct gatagcgctc
     2221 cttccgggtg tcttcttcct ctagatatcg caggttacga tccagtctcc ttcgcctcat
     2281 ggcctatcgg ggtatctgga ttgttcgcgt ggctcgcttc ccgggccatg ccgccatcat
     2341 ggacttgaga ctcagccctg cgtccacacg ctgcgtgata cctgatgaca cgttcttcta
     2401 ttgataggcc gtcccactcc attcatgtcg aactcggtgc gcgaagttac gccatcgtca
     2461 ttcgccgcga ccttcttcgg gagatcgggg aggaactgaa gcgtctcaaa tgcgcgggca
     2521 aggtaggggt cgtgaccgat cgcaatcttg ccaagcacta tctcacgcag gtgactcgca
     2581 ggctgaaggc cgccgggttt acggtcgttc ccgtcgtgct gcctcccggg gagcggacaa
     2641 aaacccttcg ctccatcggg acggtcatgg atgccctcgt gaatgccaga tttgagcgtt
     2701 cctcgacgtt gctggccttg ggtgggggtg tcatcggcga catcacggga tttgccgccg
     2761 caatctatca gcgtggcatc ccgttcgtgc aagtgccgac gagtctggtg gcccaggtcg
     2821 attcaagtgt cggcgggaag acgggtgtcg atcaccccaa ggggaaaaat ttgatcgggg
     2881 ccttcaacca gccgcgcgcg gtgttcatcg atcctgcgat gctggcaact ctgccgccgc
     2941 gcgagtgggt ggctggtctg gctgaggtca tcaaatatgg tgtgattgcg gacgcggcct
     3001 tctttgagta tctggaacag aacatggaat cgatccagaa gttgacccat gacgccgttg
     3061 cgcacatcgt gaagcggtcc tgtgagatca aggcacaggt ggtgtcggag gatgagcgtg
     3121 aggccgaccg acgccgcatt ttgaacttcg gccatacgat cgggcatgcg ttggaatccc
     3181 tcggcgggta tcggggcttc atccatgggg aggccgttgc ggtcgggatg gtttatgagg
     3241 ccgacttggc caggcacctc ggctattgtg gcgcagaggt ggttgctcga ttgcgcgcac
     3301 tggtgaaggc cgcgggtttg ccggaccggt tgccgaaggt cgcgttcaat gcgttgtggg
     3361 gtgccatgca gcaggacaag aaagtgtctg ccggaacgat tttttgtgtg gtgccggagc
     3421 agatcggggc cgtgcgcatt gttccattgg agaaggccac gacgcgcgcc tggtttcagg
     3481 atgtccacac cagagaggca gccgaattga agcgtgcaag gcccgtcgga cgggcacgtt
     3541 aggagtggaa tggcgactaa acgaacgctg actcaagtcg agcaagcctt gcgtgaaaag
     3601 acgcgtgagg tggatgtcct ccatcgcatc agcgagtcga tcagcaacac gctcgacttg
     3661 gaggccgtgc tccggcatat cgtggatatg gtcgtggaag tgaccaaggc cgacgcctgc
     3721 ttgctgtatt tgttgtcgga taacagggac gagctgatcc tgcgggcctc caagaatccg
     3781 catccgaagc tgatcgggcg gatcaccatc ggcatgggcg agggcatcac cggttgggtc
     3841 gcgcgggagc ggacgcgtgt cgtcattccc aacagcgcca gcgatgacac acggtttaag
     3901 ttctttcaca accttccgga agaccgctac caggcgttcg tctcggttcc gattacgacc
     3961 aagcaggaag tcgtcggcgt catcaacgtg cagcacaaac ggtcgcgccg gtatcggccc
     4021 gacgaattgg cgctactctc gaccatcgcc acgcaggtcg gaggggccat tgagaacgcc
     4081 cgcctgtacg atcaaatgac gcggaaggcg ttgcagctgg atacgctgtc ccaggtctcg
     4141 gagaccgtgt cgtcgaatcg attgatcgag gacgtgctgc agttgattgt gacgatgacg
     4201 gctcaaatga tgggatcgaa gatttgctcc atcattcttc tggatcagcc gaccggtgaa
     4261 cttcggatcg ccgccacaca aagtctgagc gagcagtatc ggcgcaagcc caacgtgaag
     4321 atcgggcaaa gcatcagcgg gcgtgccgtt caggatcgtc ggccgatcat cgtgccggat
     4381 gtcatcaaag aaggcaccta tatgtatccg gacatggctg cgaaggaggg cctctgctcg
     4441 ctcctgtgcg tgccgatgct ggtgcgggag aaggccatcg gcgtgatcaa tacgtatacg
     4501 tccaagccgc ataccttcac ggcggaggat gtcaaattaa tgcaggcgat tgccaatcag
     4561 gccgcgattt ccattgagca caccacgttg cttgagaaat cgtttgaaat gcaggaagcc
     4621 ttgcaggtcc gcaagttgct cgaccgggcc aagggatatc tcatgcggtc gaagcggctg
     4681 tcggaagaag acgcgttcaa attgattcaa cggcagagca tggatcttcg caaatccatg
     4741 cgagagatcg cagaagccat tctcctggcc ggagaaatcg aagagcgggc cgataagggc
     4801 aggagttgat cgccactagg cgaactttac gaccagagga gggtgcaggc cctggcagtt
     4861 ggatcgatct tcgcggtgct ggggttgagc ccgagccgtt ccttccagat tacttcacgc
     4921 ttcgtttgtc ttctgctcct cattctgctt catcggcggt actctcttgg agggctcagg
     4981 gggtgagacc gacttgtagc ccgcctgacg gtcaagtgcc acagtgaact gatttcgctg
     5041 aatgtctaca catcaatgaa cgcaacagta catgttcgag actggctggg aataggtgag
     5101 gtttggatgg cgaagagccg acgcgttgcc acagcttttc tgctaactct tatgacaagc
     5161 gcctgcgcca ccgggcaata cactccgttg gtcggggccg atcgcgatga ggtggagcag
     5221 ctcaaggaga atgtctatcg tgtggaatac cgcgtcagct cgtttacctc acaagaacaa
     5281 ctggatgcct acctgcgacg gcgttgcgcg gagctgacct tgcgcgaggg ctacgatttc
     5341 ttccacctcg cccagcgtgc cgatgtgctg gcactctcac gacgaacgtc gatgacggtg
     5401 acgatgtaca agggcaagaa gccggtgggc gctgatgacg tctatgacgc gaaggaggtc
     5461 ttggcgcagc cggacctcct ttccggcagc caatgaccgc tgccgttcgc cgttagggtg
     5521 ttgccgtcgg cttgcggggc gccgctttcg gcttgggctt ggacgcagcg acgtctttct
     5581 cagctaactg gcgccgtacc gattccaact ctgccttgag cttctggagt tcctccgcct
     5641 gtttgaccac cacatccttc gtggattgca gttcctgctg caagcggctc tgttcggcag
     5701 tgggcgaggt ttgtgtcggt gctgcctcgg cagttgtgtt gtggtcggct gcgacagggg
     5761 atggcgcagg aagctgactc agcgcctcaa gggagacggc gatacgcggc tgttccggct
     5821 cgacggtcat ccagggttga gggacatcgg ctcgcacgag ggcttgtggg gtcgtgaagg
     5881 agagtatgag cgacgcgggt ttggtctgtc catccccgga tgctgatcgg tagtgagaca
     5941 gattcactcg cagcgtggtt ttgtacacgt agagtgtgcc ttgggtcgtg agaccgtcat
     6001 cctgcatcgt gaagcgaacc cgctggtcag gttgggcctg tgcgagtgcg ccgacgatga
     6061 gcggggccag aaactccact tcctcgtgag agaacagcgg ataactctcc tgcgcgatgc
     6121 tgcttcccgg tcgatcgagt cctgaaacgg acaggccggt gagcaggcgt cccgtcagcg
     6181 aggccgtcag atgggcagga tgcgacgcct gaaaagtctg cggggtgccg tatttagcgg
     6241 tcgtaccacg gctggtctgg cgttcaagag cgacggctcc cagggggccc tgtccgacag
     6301 gagtcaggtt cgtccctagg ctgcaggcgc agaggaggag gcagggaagc gccagttgcc
     6361 aggcgagccg gaaactgctg agtcgtgatt gtgttggggt gggacggcga atcatgaacc
     6421 gcatggcggc cgattgtacc acaggctgac cgcggaaccg cctggtttcc cctggaaaag
     6481 tgcgattcaa ttggttgaca gcctgtttag gccggtggta tacacacggg ataatttccg
     6541 gggacaatgg cgtctccgac gagtaattcg ttccgaacga tacattggaa gcaggacaac
     6601 gacgtcctcc gatccaccga ggtgggtctg aggacgtttt ttttatcagc atgctgatga
     6661 atcccgtcag ctcaggatct taaagttccg atgactcgtg ttcgggctga ttgctgaacg
     6721 ctgagggctt acaggcttta gatatgcggt cgctccgaat tgccatggcc cagatcaatc
     6781 cgaccgtcgg ggatattgtc ggcaacacgc gcttgatcca gacgtggatc aaggaagcgc
     6841 gccaagccaa ggccgacctc gtggccttcc cggaactggc catcaccggc tatccgccgg
     6901 aggatctgtt gttcaagccc cgtttcatcg aggacacgca tcgtgcattg aaggcggtcg
     6961 cggcggaagc gcggggggtg gtcgtggtcg tcgggtatgt cgggcagggg gcgacggctg
     7021 tttcggcgtc ggacgcttct tcgttcccgc tgtccggtcg gcatgagtta tacaacgagg
     7081 cggcggtgct gtccg
//
LOCUS       DAORRQ010000124         7094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_415_length_7094_cov_5928.923000, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000124 DAORRQ010000000
VERSION     DAORRQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_415_length_7094_cov_5928.923000"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK224_20050"
     CDS             <1..489
                     /locus_tag="PK224_20050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB18098.1"
                     /translation="LVEAIPHVLAKFHHTTFLFIGRGELEVSLKEHVQKLGVEKKVLF
                     LGLRQDVPRLLSLMDVFVLPSLSEGLSLAILEAMTAGKPVVTTQVGGNPELVLHLETG
                     VLVPPEDSWALAEALERVLGDTGLAQRMGRAGRSRAGQYFGLHTMVKNYETLYQGSIL
                     RS"
     gene            587..2413
                     /locus_tag="PK224_20055"
     CDS             587..2413
                     /locus_tag="PK224_20055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HRB18099.1"
                     /translation="MFEFHQYDPVFHPKPLQPGGPYQPISNVAKMSLLFWSEHCIECA
                     APACFSTCDLYDRRPDSRCRRFTYGMYRNSSFSSIRGYGVEIEFKKWGKLEARANVST
                     TPMGSLLRQERLIELSLPVVNLIGTGLSRLTRDNRWSQLSYAILARLTKWLAHRSKRD
                     GCPDAFLLEVYNPADTAVKIQVRMTPANLSDRSNLIEISQPGGHFAATINLEPGYSRH
                     QMARSLFNALLESGVPFDVSLIPEGDSAPRIIILSADFVVFNGPGEKKAKAGAPDIKC
                     IVWDLDNTLWEGTLVEDPNVRLRLNAVELIKRLDQKGILMSLVSKNDEGKALEKLKEF
                     GIEEYFLYPQINWMPKSQNIQEIAKSLNIGLDTFAFIDDSPFELAEVGETLPMVRCIS
                     VDRLSSILDDPRFAGSDSPDAKNRRQFYQTAALREQTQASFGANYIAFLAHSNIKLTI
                     KKYESGDFERVAELVQRTNQLNFSGKKYKREEIADLVTRPGIDKYLLACNDKFGSYGT
                     VGFSIVKQTSAEIEVKDFMLSCRVQGKFIEKAFFGFLVKCQGPEAVLRFRVGFMQTSR
                     NAPAKQVLDKLNFVSAEDGVGLVLDCPGESLACDFIQVEYQN"
     gene            2454..3212
                     /locus_tag="PK224_20060"
     CDS             2454..3212
                     /locus_tag="PK224_20060"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRB18100.1"
                     /translation="MVIINSDDLGMNPAVNERILDLMSRRRITSASILANGPAVEQAI
                     RLIPKSSGCSLGVHLNLTEFEPLTPPRHLGDLRACLNENGVFAGERSLSNLSMSRSVQ
                     EGIYRELRLQVQKVIALGVKISHFDSHNHIHTIPQVFLVLKRLQKEFGVRKVRTTWNI
                     YPPQKPVSTALRIRKKVWDFALRRLYRTRTTQGLTTFSIFYDLAKRKQLLCHSIELMT
                     HPGHAEYEEETRLLDHDWENEILFPVDLVNYHAL"
     gene            3309..4421
                     /locus_tag="PK224_20065"
     CDS             3309..4421
                     /locus_tag="PK224_20065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB18101.1"
                     /translation="MHLITLLQALSKMQDLDMSAVLFTEGRVAEELRATGLDVVVIDE
                     HENNWFAILWKLVKHFREHQFMVVHAHKPKDNFLGGVACKLAGVPYFVRTIHGSWEPF
                     SGFEHFKIKIYEHIDRLANRYLVHAVIAVTMKIQSLLAKGCPSEKVVCIHNGIDVERL
                     MVSRGRQGVRRDLDISERTVLLGAVGRLTAVKGHIHLLEAMRILVSKQIDTHLVILGD
                     GPLRKQLEGMVAEYKIQKNVDFVGHQSTVSDFIEAMDIFVLPSLHEGIPMVLLEAMAL
                     SRPIVASKTGGVPEVIQHGENGLLVEPGNPEAIAEAIETLIRQVANADQLGRNGRIRV
                     TQNFTANIMAEKTADLYRRLVGSRFANSATQVNPIN"
     gene            4434..5633
                     /locus_tag="PK224_20070"
     CDS             4434..5633
                     /locus_tag="PK224_20070"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRB18102.1"
                     /translation="MVLMLGTGCFLVLVLLSIMSLYQWGHAILAISSKKSASNQRSAR
                     TTRFLILIPAHNEEDGLPETLKSLSTIQYPKDLVHIAVIADRCADDTAKVARAGGAQC
                     LERSEGPGGKGAAMSWAMQVLRNEGTLFDALVIVDADCLADLHLLEAFDDALAKGHEV
                     QQGYNYLSNPWETPFTRVIAVTSVLRNFLFYGGKEAMGCSAMLSGTGMCLSRSVVDQH
                     GWSAFSVAEDWEFSVSLLLNDVIIHFNPLARVYARESKGLKQASRQRLRWATGKYAVM
                     TNGARRLFLQGLSRGKVNLIDGAVTLAAPNYSSQASMTLFALVCSLFLSQDPAWGFLL
                     PWSLAVLGSLGGYFLLGAMHTQSPLKTLAGIPYIAVFLPWRLSIEILGFLGFGRKGWG
                     RTFRNPR"
     gene            5755..6765
                     /locus_tag="PK224_20075"
     CDS             5755..6765
                     /locus_tag="PK224_20075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB18103.1"
                     /translation="MGKRRLRVITDLANLDGFETDEYVVEARQIEGDESFGGGARLFR
                     QAFGYDIVLLNSSTRRLLALCAMMWLLPIYRWQLISLDIHLLEPVGWKRRIVALIVRI
                     LLRKVHLHILYFKDLLGYEKYYGVSHAVSRFVPFKVNSWEILPSRESLDSDGEYVFCG
                     GRSLRDLKTFKAAMAKVPYPGLLLYQHDASFMETYGTAVDLSDLPSNIRPTCHDGNND
                     TWIDFIKRAKLIVIPALPNSIYAPGLSLYLLAMALRKCVIISEGPQTRGLLKDEAVIV
                     PPSDPEALADAIRRSWEDDNFRRTTADAGRRYAELCAGESRMLHDIVRICGEVHRRDS
                     PV"
     gene            complement(6811..>7094)
                     /locus_tag="PK224_20080"
     CDS             complement(6811..>7094)
                     /locus_tag="PK224_20080"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRB18104.1"
                     /translation="CHTVSAMTLDRIIDEYNLARVNVLKIDIEGAEREVFSDTSAWLE
                     KIDSIIIELHEHMKAGCNRNFYCGSKGFDNEWKKGENVYLSRGNIRPSV"
BASE COUNT         1764 a         1560 c         1888 g         1882 t
ORIGIN      
        1 ttagttgagg caattcccca tgtcttagcg aaatttcacc ataccacgtt cttgtttatt
       61 ggtcgaggag agctcgaagt aagcctcaaa gaacatgtgc agaagctggg agttgaaaaa
      121 aaagtcttgt tcttgggcct gaggcaagat gtcccacgct tactttcatt gatggatgta
      181 tttgtcctgc catctctttc ggagggactt tcgctggcga tcttggaggc aatgaccgct
      241 ggtaaaccag ttgttactac tcaagtagga ggaaatcccg aactggtcct tcatttagag
      301 acgggcgtgc tcgtgcctcc agaggacagt tgggcgctcg cagaagctct tgagcgggta
      361 ctgggtgaca ctggactcgc gcagcgaatg ggccgcgctg gcagaagtag ggccggtcag
      421 tatttcggcc tgcataccat ggtgaagaat tatgaaacgc tgtatcaggg ttccatcctc
      481 cggtcataga atgacctcga agcgatcacg aaggatgtcg ggctaagatt aagatacagt
      541 tatggcatcg tactgagttt cgtgatgggc gaaagaggga agtgccatgt ttgaatttca
      601 ccaatatgac ccagtgtttc atccgaagcc tcttcagcca gggggtcctt accagccgat
      661 ttctaacgtc gcaaaaatgt ctctattgtt ttggagtgag cattgtattg aatgtgcagc
      721 acccgcttgt tttagtacct gcgaccttta tgatcgaagg ccagatagtc gttgccgtcg
      781 atttacctat ggaatgtata gaaattcaag cttctcctcc atccgtggtt acggagttga
      841 aatagaattc aagaaatggg gcaagcttga ggccagagca aacgtttcca cgacgccaat
      901 gggctccctt ctgcgccaag agcgcttgat cgagttgtcc ttgccggtgg tcaatcttat
      961 cggcacgggc ctaagccggc taactcggga taaccggtgg agtcagctct cctatgcgat
     1021 tcttgctaga ctcactaaat ggttggctca ccgctccaag agagatggtt gccccgatgc
     1081 atttcttctt gaggtgtaca accctgctga tacggccgta aagattcagg taagaatgac
     1141 gcctgctaat ctgtctgacc ggagcaatct tatcgagatc agccagccag gtggtcactt
     1201 tgcggctacc ataaacctag agccgggata ctccaggcac cagatggccc gctccctctt
     1261 caacgctctg ctggagtcag gagtgccctt tgatgtgtcc ctaatccctg agggagacag
     1321 cgcccctcga ataataattt tgagcgccga tttcgtcgta ttcaatggcc cgggtgaaaa
     1381 gaaggcgaaa gcaggagccc ccgatattaa gtgtattgtt tgggatctgg acaatacact
     1441 ctgggaaggc accttagttg aagaccccaa tgttcggctg agattgaacg ctgttgaatt
     1501 gatcaagagg cttgaccaga agggcattct tatgagtctt gtgagcaaaa acgatgaagg
     1561 gaaagccctt gagaagctga aggagtttgg gattgaagag tacttcttgt atcctcagat
     1621 caactggatg ccaaaaagtc agaacataca ggaaatcgcc aaatctctta acattggctt
     1681 ggacaccttt gcgttcattg atgacagtcc atttgagttg gctgaagttg gcgaaacact
     1741 gcctatggtg cggtgcattt cagttgaccg tctgagttcc attctagatg atccgcgctt
     1801 cgccggaagc gactcaccag atgcgaaaaa tcgtcgtcag ttttatcaaa ctgcagcgct
     1861 gagagagcag acgcaggctt cttttggagc taattacatt gcatttcttg cacacagcaa
     1921 tattaaattg accatcaaaa aatacgaatc tggtgatttt gaaagagtgg ctgaactggt
     1981 gcagcgcacc aatcagctaa atttttccgg gaagaagtac aaaagagagg agattgctga
     2041 tctggtcacg agacctggga ttgataaata tctactggct tgcaatgaca agtttggttc
     2101 atacggtaca gtagggttta gtattgtgaa acaaacctct gcagaaattg aggttaagga
     2161 tttcatgctg tcctgtaggg ttcaagggaa attcattgag aaagcatttt tcggttttct
     2221 tgtgaagtgc caaggccctg aggccgtact tcgttttcga gttggtttca tgcaaactag
     2281 tcgcaatgct cctgctaaac aagtgttgga caagcttaat ttcgtgagcg cagaagatgg
     2341 tgttgggcta gtgcttgatt gtccaggcga gtcccttgcc tgcgatttca tccaagtaga
     2401 atatcagaat tagatgcgag catgctatgc ctcccagtgc tggtgcacgc gtgatggtaa
     2461 tcataaatag tgacgatctg ggtatgaatc ctgccgtgaa cgagcggatt cttgatctca
     2521 tgtccagacg aaggattacc tcggcaagca ttcttgcgaa tggtcctgcg gttgaacagg
     2581 ctatcaggct gattccgaaa agttcgggat gctctctcgg cgttcatctc aacctcacgg
     2641 aattcgaacc actcacgcct cccagacatc tcggtgacct gcgcgcttgc ctcaacgaga
     2701 atggtgtgtt cgccggtgag cgcagcctga gcaacttgtc gatgtcacga tccgtgcagg
     2761 agggaatata tcgggagtta cgacttcagg tgcagaaggt aatcgctctt ggggttaaaa
     2821 tttcgcattt cgactcacac aaccatatac acactatccc tcaagtattt ctggtactta
     2881 agcgtctgca aaaagaattc ggggttcgta aagtgcgcac gacctggaat atttaccctc
     2941 cacagaaacc tgtttccaca gcacttcgaa taaggaaaaa agtgtgggat tttgccctgc
     3001 gccgcctgta caggacgaga actactcaag gactaacgac gttctcgatt ttttatgatt
     3061 tggcgaaaag gaaacaactg ttgtgtcatt caattgagct tatgactcac cccggacatg
     3121 ccgaatacga agaagagacc aggttgttag accacgactg ggagaacgag attctttttc
     3181 ctgtcgacct cgtaaattat catgcactct agctcacctg ggtatcggga ggtgtcgact
     3241 atacgggtac ccaagattgc aaaggtgtgc catattgcag tggctgatct ttgggggggg
     3301 gggcagaggt gcatctgata actttgctgc aagcactgtc caagatgcag gatctagata
     3361 tgtccgcggt tctttttact gagggaaggg ttgcggagga attgagagct acgggcctag
     3421 atgtggttgt tatcgatgag catgaaaaca actggttcgc cattctgtgg aaactcgtaa
     3481 agcactttcg tgaacatcag ttcatggtgg tgcacgccca caaacctaaa gacaatttcc
     3541 tgggcggagt ggcgtgcaag ttggccggtg tgccctactt tgtcagaacc attcacgggt
     3601 cgtgggagcc cttttcgggc tttgagcatt tcaagattaa aatttatgaa catattgatc
     3661 gattagccaa tagatatttg gtccacgcag taatcgccgt aacgatgaag attcaatccc
     3721 tgctggccaa aggatgccca agcgagaaag tggtttgtat ccataacggt atagatgtgg
     3781 aacgccttat ggtcagcagg ggaaggcagg gtgttcgaag agatctggac atcagtgaaa
     3841 gaacggtgtt gttgggtgct gttggaagac tgacagcagt gaaaggccac attcacttgc
     3901 ttgaagctat gcggattctt gtaagtaaac aaattgacac acatcttgtc attctggggg
     3961 atggtccact gcgaaagcag ttagagggta tggttgcaga gtataaaatt caaaagaacg
     4021 ttgattttgt tggtcatcag agcactgtaa gtgattttat cgaggcgatg gatatttttg
     4081 tcctcccctc actgcatgaa gggataccga tggtattgct cgaggctatg gcactgagtc
     4141 gtccaattgt ggctagtaaa acaggtggag tgccggaagt catacaacat ggagaaaatg
     4201 ggttgttggt tgagcctgga aatcctgagg cgattgctga ggctattgag acactcatca
     4261 ggcaggtagc aaacgcggac cagctaggtc ggaatgggcg gattcgggtt acccaaaatt
     4321 tcactgcaaa cataatggca gaaaaaacgg cggatctcta ccgtcgattg gtgggatcca
     4381 gatttgcgaa cagtgccact caagttaacc ctataaatta aaagttggct acgatggtac
     4441 tgatgctggg cactggttgt tttcttgttt tggtactgct ttccattatg tcactctatc
     4501 aatgggggca cgcgattctc gcgatatcaa gtaagaaatc tgcctcgaat cagagaagtg
     4561 cacgtaccac tcgttttctg atcctgattc cggctcacaa tgaagaagat gggttgccgg
     4621 agactctgaa gagtttatcc accatccagt atcccaagga ccttgtgcat atcgccgtga
     4681 ttgcggatcg ctgtgctgac gacacggcaa aggtggcgag ggccggtggt gcgcagtgcc
     4741 tggagcggag tgaagggccc ggtggaaaag gtgcggccat gtcgtgggcg atgcaggtgc
     4801 ttcgcaatga aggcacattg tttgatgcgt tggtcattgt tgacgcagat tgcctagcag
     4861 acttgcattt gctggaggct tttgatgatg ctctggcgaa agggcatgag gttcagcagg
     4921 gatataacta tctctctaac ccgtgggaaa ctccctttac cagggtgatt gcagtgacga
     4981 gtgtcctgcg aaacttctta ttttatggag gtaaggaggc aatgggttgt tccgccatgc
     5041 tgagcggaac cggaatgtgt ctgagtcggt cagttgtgga tcagcatggg tggtcggctt
     5101 tttctgtggc ggaggactgg gaattttcgg tgtcactgct gctgaatgac gtcataattc
     5161 atttcaaccc cttggctcgt gtctatgcca gggagtctaa ggggttgaag caagcttctc
     5221 gccagagatt gcgatgggcg acaggcaagt atgcggtgat gacaaacgga gcaagacgtc
     5281 tgttcttgca gggattgtcg cgtggcaagg tcaatttgat agatggggca gtcaccttgg
     5341 ccgctccaaa ctattcgagc caggcctcca tgaccctctt cgctcttgtc tgcagccttt
     5401 ttctgagcca ggatcccgct tggggatttc tcctcccatg gtcgctggca gtgcttggat
     5461 cactgggagg gtactttctg ttgggcgcga tgcacacgca gtcaccatta aaaacgttgg
     5521 ctggtattcc ctatatcgcg gtgtttctac cgtggcggct ctcaatcgag atattaggtt
     5581 tcctcggatt tggtcgaaaa ggctggggcc gcacgttcag aaatcccaga taatgcggga
     5641 attgaagtag gaccgacaag gcacatccct aagcaacggg tgttgtcgat gcaataaaca
     5701 tacagccaac cggctgccag tcattattga caagcgagat ttaggtgagg atctatgggc
     5761 aaaagaaggc tgcgcgtcat aacagatcta gctaacctcg atggctttga aaccgatgag
     5821 tatgttgttg aggctcgcca gattgaggga gatgaatcgt ttggcggcgg agcaagattg
     5881 tttcgccagg catttggcta tgatatcgtg ctgctgaata gcagcacgag acgattactt
     5941 gctctttgtg caatgatgtg gttgctgccc atttatcgct ggcaacttat ctccctagat
     6001 atacatctac ttgagcccgt agggtggaaa cgtcgtatag tcgctctgat cgtacggatt
     6061 ctgctccgta aggtacattt gcatatactc tactttaagg acttgttggg gtatgaaaaa
     6121 tattacggtg tttcacatgc cgtctctcgc tttgtgccat tcaaggtgaa ctcttgggaa
     6181 atccttcctt cgcgggaatc gcttgattca gatggcgagt atgttttctg tggagggaga
     6241 agtcttcgag atctaaagac ttttaaggcg gccatggcta aagttccata ccccggcttg
     6301 ctcctttatc agcatgatgc atccttcatg gagacatacg gaaccgcggt agatctaagc
     6361 gacctcccaa gtaatatccg accgacttgt cacgatggaa ataacgatac ttggattgac
     6421 tttatcaaac gtgccaaatt aatagtgatc ccggcccttc caaacagcat ttatgctcca
     6481 ggcttgagcc tctacctctt ggcaatggcg ctgagaaaat gcgttatcat atctgaagga
     6541 ccacagactc gtgggctgtt aaaggatgaa gcggtcatcg tgccccccag tgatccagaa
     6601 gcattggctg atgctattcg tcggagttgg gaagatgata attttcgcag aactaccgcc
     6661 gatgcaggaa gacggtatgc agagttatgc gcgggcgaat ccagaatgct ccacgatatt
     6721 gtaaggatct gtggggaggt acatcggaga gatagtcctg tgtaagcttc cctgtcaacg
     6781 agacacttcc cccgtctatt acaccagatc ttacactgag ggtctgatat ttcctcttga
     6841 taagtatacg ttctcccctt ttttccattc gttatcaaat ccctttgagc cacaataaaa
     6901 gttgcggtta caccctgctt tcatatgctc atgaagctca ataatgattg aatcgatctt
     6961 ttcaagccag gccgaagtat cactaaatac ttctctctct gctccttcga tatcaatttt
     7021 gagaacattt actcttgcga gattgtattc atcgattatc ctatcaaggg tcatggccga
     7081 aacggtgtga caag
//
LOCUS       DAORRQ010000125         6601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_438_length_6601_cov_55.466697, whole genome shotgun sequence.
ACCESSION   DAORRQ010000125 DAORRQ010000000
VERSION     DAORRQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_438_length_6601_cov_55.466697"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1847
                     /gene="oadA"
                     /locus_tag="PK224_20085"
     CDS             <1..1847
                     /gene="oadA"
                     /locus_tag="PK224_20085"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HRB18105.1"
                     /translation="PGKKLLMTEVALRDGHQCLLATRMRTEDMLPIAQKLDAVGFWSL
                     EVWGGATFDTCLRFLKEDPWERLRALRAAMPKTKLQMLLRGQNLVGYRHYADDVLEKF
                     IERSAFNGIDVFRIFDALNDVRNLERAIREVKACEKHVEAAISYTTSPVHRLDGFVTM
                     GKRLEDLGADTICIKDMAGLLAPADAYRLVKSLKAAVRVPIHLHSHYTSGMGTMSALM
                     AVMAGLDLLDTSISPLAGGASHPPTESMVAALRGTPYDSGLDLEDLQPIAEHFRNVRR
                     KYRQFESDFTGVDAEILTSQIPGGMLSNLAAQLAEQNALDRMKEVMDEIPRVRKDMGY
                     PPLVTPTSQIVGTQATLNVLTGEQGERYKVITTETKNYFLGLYGRAPGPLDKEIMARA
                     IGDEEPVKGRPADRLESEFEKLKKDMPESATTLEDQLSFALFPAIARDFFEARERGDL
                     RAEPLEPTETKGPAVAHDLHLAPAEFNITVHGENYHVVVSGSGRTTDGRKPYYIRVND
                     RLQEVSLEPLQEVLAGVPESPGAGSTSKPKRPRPTKPGDVAPPMPGRVVKVLVTDGAQ
                     VKTGDPLLIIEAMKMESQVPAPMDGRVTAILVVEGDSVKIDETVIQLE"
     gene            1931..2815
                     /locus_tag="PK224_20090"
     CDS             1931..2815
                     /locus_tag="PK224_20090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB18106.1"
                     /translation="MSGCAAPPEIPCWFDGFQRFPIRTTSVNGHRIAYLDEGQGPPLI
                     LLHGYGGSMWQWEYQQLPLSRQFRVITPDLIGSGLSDKPPLDYRPEELIESIRGLMDA
                     LGLPTATLIGNSMGGGVAIGMALTHPDRVSRLVLIDSLPDHVRERLVSPLMQRALNTS
                     VPAWLARFGALFVGNRTMEAVLKEIIFDHTLVTAAVLDRSNRNRQREDMITPLMSLRD
                     SLPLWEQHFAPRLKEIRHPTLILWGEQDRLFPPQVGRDLQTTIPQARLIMIPEAGHIP
                     QWEQPQVVNRHITEFLQP"
     gene            2907..3572
                     /gene="tpx"
                     /locus_tag="PK224_20095"
     CDS             2907..3572
                     /gene="tpx"
                     /locus_tag="PK224_20095"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRB18107.1"
                     /translation="MIPGASTSHFLAALLYLGLSGCGTLPSSGHSDFSYKNMPVADGS
                     AVAGEGNNVLFQGKPLMLSGMGIKVGDKLRDVKLTQTDLSMVPINDTKGKGKVRIISI
                     VPSLDTKVCEQQTHYLSEKNMGLDRMVELITISIDTPFAQKRFAEEAKIANVTFLSDF
                     RAADFGKAHGLLLKDPHLLSRALLVVDKDNKVRYLQITPELAQLPDIEEAFRFARKLV
                     TAS"
     gene            3744..5147
                     /gene="sthA"
                     /locus_tag="PK224_20100"
     CDS             3744..5147
                     /gene="sthA"
                     /locus_tag="PK224_20100"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HRB18108.1"
                     /translation="MAHYDLLVIGTGPAGQKAAIQAAKLGKKVGIVERKRVVGGVCTN
                     TGTIPSKSLREAALYLSGFHQRSLYGASYRVKQDITMEDLTFRANHVINREIEIIQNQ
                     MTRNNVDLWFGTAGFIDPHRLRIERSDDLVEHTADFVVIACGTVPARPSHIPFDDHSI
                     IDTDGLLTLKTLPKSITIIGGGVIGAEYASILATMGIHVTLIERRPRLLEFVDQETIE
                     ALQYHMRSIGVTLRFNEEVVSVERQPQEQVIVRLKSGKEIAATTVLYSVGRTGASATL
                     NLEAIGLTADDRGRLKVNEHYQTTVPHIYAAGDIIGFPALASTSMQQGRHAACHAFGI
                     PCQTQSELMPYGIYSIPEISMVGRNEDDLTKNGVPYAVGIARYREIARGQIIGDELGM
                     LKLLFHNKTRQLLGVHAIGDGATELIHIGQTVMAYQGQIDYFIEAVFNYPTLAECYRV
                     AALDGINQLPRPWPPRV"
     gene            5272..5940
                     /gene="tenA"
                     /locus_tag="PK224_20105"
     CDS             5272..5940
                     /gene="tenA"
                     /locus_tag="PK224_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HRB18109.1"
                     /translation="MSFSNHLRKLAQPVWDAQLTHPFVVALGKGTLAERKFRYYILQD
                     ARFLADLARVFAAGSLRAPDSESALRFAKLAEETITVERSLHENYGKRWKLSPHDMLN
                     EPMAPTNYAYTRHMLAVAQGGTATEIAVVALPCAWIYCVVGKHFLKNGPPSAKHPYRD
                     WLMLYASPEFEAVQEWMRARVDTWARTAGHEEKKRMEQAFLISSKYEWMFWEMAWNEE
                     KWPV"
BASE COUNT         1437 a         2110 c         1875 g         1179 t
ORIGIN      
        1 cgccggggaa aaagctgttg atgaccgagg ttgcgttgcg cgacgggcat caatgcctgc
       61 tcgcgaccag gatgcgcacc gaggacatgt tgcccatcgc ccaaaagctg gacgccgtgg
      121 gattctggtc gttggaggtc tggggcggcg ctaccttcga tacctgcctc cggttcctca
      181 aagaagatcc gtgggaacgt ctgcgcgccc tccgcgcggc gatgccgaag acgaaactgc
      241 aaatgttgtt gcgcggccag aacctggtcg ggtatcgcca ctacgccgac gacgtgctgg
      301 agaagtttat cgagcgctcg gcatttaacg gcatcgatgt cttccgcatc ttcgacgccc
      361 tcaatgacgt ccgcaatctg gagcgggcca tccgtgaagt caaagcctgt gaaaagcatg
      421 tagaagcggc catctcctac accaccagcc cggtccaccg gctggatggg ttcgtcacga
      481 tgggcaaacg attggaagac ctgggcgccg ataccatctg catcaaagac atggccgggc
      541 tactggcgcc tgccgatgcc taccgtctgg tcaagagcct caaagcggcg gttcgcgtgc
      601 ccatccacct gcactcccac tacacgtcgg gcatgggaac catgtcggcc ctgatggcgg
      661 tcatggccgg actcgatctc ctggacacct cgatttctcc gcttgccgga ggcgcctcgc
      721 atccccccac cgaatccatg gtggctgcgt tacggggcac gccctatgac agcggattgg
      781 acctcgaaga tctgcagccc attgcagagc atttccgaaa cgtgcgccgg aagtaccggc
      841 agtttgaaag cgacttcacc ggtgtggacg ctgaaattct gacgtcccag attcccggcg
      901 gcatgctctc caatctcgcc gcgcaattgg ccgaacaaaa cgccttggac cgaatgaaag
      961 aagtgatgga cgaaatcccc cgtgtccgca aagacatggg ctatccgccg ctcgtcacgc
     1021 cgaccagcca gatcgtcggc acgcaggcca ccctcaacgt gctcacgggt gaacagggcg
     1081 agcggtacaa ggtcatcacc acggaaacca agaattattt cctcggcctc tacggtcggg
     1141 ctcccgggcc gctcgataaa gaaatcatgg cgcgggccat cggggacgaa gagcccgtga
     1201 agggccgacc tgccgaccgg cttgaatcgg aatttgaaaa actcaagaag gacatgcccg
     1261 agtccgccac gacgctggaa gaccaactgt cgttcgccct cttccccgcg attgccaggg
     1321 atttcttcga agcacgcgaa cggggcgacc tgcgggcaga gccgctggaa ccgacggaaa
     1381 cgaagggtcc tgccgtagcc cacgatctcc acctcgcgcc ggctgaattc aacatcaccg
     1441 tgcacggcga gaattatcat gtcgtggtct cgggctcagg ccgcaccacc gacggccgca
     1501 agccttacta cattcgggtc aacgaccggc tgcaggaagt ctcactggaa ccgctgcagg
     1561 aagtgctggc cggtgtgcca gaatcccccg gagccggcag cacgagcaag ccgaaacgtc
     1621 cccggccgac caaacccggc gatgtcgccc cgcccatgcc gggtcgtgtc gtgaaagttc
     1681 tggtgacgga cggcgcccag gtgaagaccg gtgatccgct cctgatcatt gaggccatga
     1741 agatggaaag ccaagtcccg gcgccgatgg acgggcgagt cacggccatt ctggtcgtcg
     1801 agggagacag cgtcaaaatc gacgaaaccg tcattcaact ggagtagccg tgaggctccc
     1861 ggcgtggcag gtgcggtgta ccgccgtcgc gtcgtcacac gtcctaacct tgtgtctcgc
     1921 ggcggtcctc atgagtggtt gtgcggcgcc tccagagatt ccctgctggt tcgacggctt
     1981 ccaacgattt cccatccgca ccacctcggt caacggccac cgcatcgcct atctcgacga
     2041 aggccagggc ccgccgctca tcctgctcca cggatatggc ggttcgatgt ggcagtggga
     2101 atatcagcag cttcccctct cccgacagtt ccgcgtcatc acgcccgacc tgatcgggtc
     2161 aggcctctcg gacaaacccc ctctcgacta tcggcctgaa gagttgatcg aatcaatccg
     2221 cggcctcatg gatgcgctcg gacttccgac cgccacctta atcggcaact ccatgggagg
     2281 cggcgtggcc atcggcatgg ccctgaccca tcctgatcgg gtgtcccgcc tggtgttgat
     2341 cgacagtttg cccgatcatg ttcgcgaacg actggtcagt ccgctgatgc aacgcgccct
     2401 gaacaccagc gtccccgcat ggctggcccg cttcggagcc ctgttcgtgg gcaaccgcac
     2461 catggaggcc gtgctgaaag aaatcatttt cgaccacacg ctggtgaccg cggcggtgct
     2521 ggaccgctcc aaccggaacc gtcaacgcga ggatatgatt acgcccctca tgtcgctccg
     2581 ggacagcctg cccctgtggg agcagcactt tgcgcccagg ctcaaggaga tccggcatcc
     2641 caccctgatt ctctggggcg aacaggatcg ccttttcccc ccgcaggtcg gccgcgacct
     2701 ccaaaccacg attccgcagg cccggctcat catgatcccc gaagccggcc atatcccgca
     2761 gtgggaacaa ccgcaggtgg tgaaccggca cattacggaa ttcctgcaac cttgacagca
     2821 tgccgaaccg gccatacagt gacagagcac actgagccta cattcctctt cgatggagat
     2881 attctatggc atcgccccag acccgtatga ttcccggcgc ttcgacgtct cactttttag
     2941 cggccctgct atacctgggc ctcagcggct gcggaacctt gcccagctcc ggccactctg
     3001 atttttcata caagaatatg ccggtcgccg acggcagtgc cgtagccgga gaaggcaaca
     3061 acgtcctgtt tcagggcaag ccattgatgc tctcggggat gggaatcaaa gtcggcgaca
     3121 agctgcgcga tgtgaagctc acccagaccg acttatcgat ggtgccgatc aacgacacga
     3181 aagggaaggg caaggtccgt attatcagta tcgtgccgtc cctcgacacc aaggtctgtg
     3241 aacaacagac ccactacctg agcgagaaga acatgggcct cgaccgaatg gtcgaactga
     3301 tcaccatcag catcgatacg ccctttgcgc aaaaacgttt cgccgaagaa gcgaagatcg
     3361 ccaatgtgac cttcctctct gatttccgcg ccgccgattt cggcaaggcc cacggacttc
     3421 tgctcaaaga cccccatctg ctgagtcgcg ccctgctcgt agtggacaag gacaacaagg
     3481 ttcggtatct ccaaatcacc cccgaactcg cgcagctgcc ggacattgag gaagcattcc
     3541 ggttcgcgcg taaactggtc acggccagct aaggacagcg tccaccgtga agcgtcgccc
     3601 ctctctcgcg aatgcagaga agagcgtatg gcgaatggct gatagcgcgg aaggacaggg
     3661 aatcttctct atgagtgcga gatttcgttt accggcgagt tgcacttcac gcttcccgaa
     3721 gcacggatac taatcaagac accatggcac actacgatct gctggtcatt ggaaccggcc
     3781 cggcagggca gaaagccgct atccaggcgg ccaagctggg aaagaaagtc ggcatcgtcg
     3841 aacgcaaacg ggtcgtcggc ggcgtctgca ccaacaccgg caccatcccc agcaaatcgc
     3901 tccgcgaggc cgcactctat ctgtcgggtt tccatcagcg cagcctgtat ggcgccagtt
     3961 atcgcgtcaa acaggacatc acgatggaag atctcacctt ccgcgccaac cacgtcatca
     4021 accgggaaat cgagatcatc cagaaccaga tgacgcgcaa caatgtcgac ctctggttcg
     4081 gcaccgccgg tttcatcgat ccgcaccgcc tgcgcatcga acgctccgac gacctcgtgg
     4141 agcacacggc cgacttcgtg gtcatcgcct gcgggacggt tcccgcccgg ccctcacata
     4201 ttcccttcga cgatcacagc atcatcgaca ccgacggcct gctgaccctg aaaacactcc
     4261 ccaagtcgat tactatcatc ggcggcgggg tgatcggcgc ggaatatgcc tcgatcctgg
     4321 cgaccatggg cattcacgtc accctcatcg aacgccgtcc gcgcctgctg gagtttgtcg
     4381 atcaggaaac gatcgaggcc ctccagtacc acatgcgcag catcggcgtg accctccggt
     4441 tcaacgaaga agtcgtgtcc gtcgagcgac agccgcagga gcaggtcatc gtccgcctga
     4501 aaagcggcaa agaaatcgcc gccaccacgg tactctattc ggtcggccgc accggcgcca
     4561 gcgccaccct caacctggag gcgatcggac tcacagcgga cgaccgtgga cgactgaagg
     4621 tcaacgagca ctatcagacg acggtgccgc acatctatgc agcgggcgac atcatcggct
     4681 ttcccgcgct cgcctcgacg tccatgcagc aaggacgcca tgccgcctgc catgccttcg
     4741 gcattccctg ccagacccag tccgagctga tgccatacgg catttattcg atcccggaaa
     4801 tttccatggt cggccgcaac gaggatgact tgaccaaaaa cggcgtcccc tatgccgtcg
     4861 gcatcgcccg ctacagagag atcgcccgtg gacagatcat cggcgacgaa ctcggtatgc
     4921 tgaaactgct ctttcacaac aaaacccgac aactgctcgg cgtgcatgcg atcggcgacg
     4981 gcgccacgga actcatccac atcggccaga ccgtcatggc ctatcagggc cagatcgatt
     5041 atttcatcga ggcggtgttc aactacccga cactcgcgga gtgttaccgc gtcgcggccc
     5101 tcgacggcat caatcagtta cccagaccct ggccaccgag agtctgacgc gtcgttcgtg
     5161 aaacgtgaag cagacaatga atgaaccggc aggagtctgt atcgggaata gcccgcgaga
     5221 tacgcttcac gatcgacgag gtacgcgtga cgtcgacaaa ggaggaaccg gatgtcgttt
     5281 tcgaatcatc ttcgtaaatt ggcccagccc gtctgggatg cccagctgac acacccgttc
     5341 gtcgtggccc ttggaaaggg tacgctggcg gagcggaagt tccgctacta tatcctgcag
     5401 gatgcccgct ttctcgcgga tctcgcccgc gtatttgccg ccggatcttt gcgggcaccc
     5461 gactcggagt cggccctgcg cttcgccaag ctggcggaag aaaccatcac cgtggaacgc
     5521 agcctccacg agaactacgg caagcgctgg aaactctccc cccacgatat gctgaacgaa
     5581 ccgatggctc ccaccaacta tgcctatacc cgccacatgc tcgccgtcgc tcaaggcggg
     5641 acggccaccg agatcgccgt cgtggcgctg ccctgcgcct ggatctactg cgtggtcggc
     5701 aaacatttcc tcaagaacgg accgccgtct gccaaacatc cctatcggga ctggctgatg
     5761 ctctatgcct cgccggagtt cgaggccgtc caggaatgga tgcgggcccg ggtggatacc
     5821 tgggcgcgca cggcgggcca cgaggaaaag aaacgcatgg agcaggcgtt tctgatcagc
     5881 tcgaagtacg agtggatgtt ttgggagatg gcctggaacg aggaaaagtg gccggtgtga
     5941 gggggcgggc aggcatggca acactctgca cgccgggaac aaagcaccag caggattcgt
     6001 cagtgagata agctttgtcg cctcaacttg ctccgttccg tgcaccgcac gaacggagct
     6061 tcgaacaaga tgcgccacct gcgagaagca ggacagctca cctcgctgcc gaacgagttc
     6121 ctgctgctgc tttgagggcg ttccaagtgc cgccacaccc acccgccagt cgcacgtcca
     6181 cggaatgagg agtagaaaga aagagagaga tggccactgt agtgggagga atggcaggca
     6241 ggttggacgc gcgcagtttg gccaagcctt gttacctacc ccgaagaaaa ccattggcca
     6301 ccacagagag ggagcaggtg ctaacagata gggccgcccg tgaaacacac ctccagaacc
     6361 agaatggata aatgctatgc tggctggatg tccctctgct cgcagaacgc gcacagtcag
     6421 ggtgctcgtc cgatgcgcgc acaccggggc aatccaacca gctgctcatt acttgctcgt
     6481 ccagaatgag gaatgacggc gacgagattg ggattaagcc ctcagcactg caccactcac
     6541 gaacgacgac cagctaccaa ggatggacgg tgaagaaagg aaagtaccac gccttgcttt
     6601 a
//
LOCUS       DAORRQ010000126         6146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_457_length_6146_cov_8.377278, whole genome shotgun sequence.
ACCESSION   DAORRQ010000126 DAORRQ010000000
VERSION     DAORRQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_457_length_6146_cov_8.377278"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1646
                     /locus_tag="PK224_20110"
     CDS             153..1646
                     /locus_tag="PK224_20110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRB18110.1"
                     /translation="MDADESTGPTSQIPAAPLPCVGAPPPPHAYDPGFSRAITWLSAV
                     VLSASIAVVAWLSFDVPRVDRVPDAERALSHMVGRLMDQADGLKTLPVWEQFLYEATM
                     GSDANDREQAIEWYRELTEESSDPSVDLHLAILEAESGYLPAVQQKMARWVRQGEPYP
                     TFARLLQAGYVDPRVPPASGFELQAYLAEQSVSGWFYSRLATRIAERAGDRPLLVTIE
                     TSLQQRVEALLWRSRAFALLELTLMIVGLFLLVLWVRRGQGTAMFRVGSAELPPLWAG
                     RLGAGVLLRGGAVGALLTVAFLYVAGDYPSLRVVAVPLSNLPLLALAYYHLLRPQRQT
                     FWRGFGLRIEPRHLGQLGLAVLAVVAAGLVGEWVLGRIAEPLNLISHWTEWFDADLVW
                     GSSPTLMVSLMEYVFFAPVFEELAFRGLLFGVFRRRFQWGVAAMLSAALFALAHGYGL
                     IGFLSVFWSGVIWAWAYERTGSLWPGMIGHAINNLLVCLSVMALLRA"
     gene            complement(1666..2484)
                     /locus_tag="PK224_20115"
     CDS             complement(1666..2484)
                     /locus_tag="PK224_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18111.1"
                     /translation="MTTPRALVILGSGYTGRWIYKLAEQQSLPILASSRHPDRHLNDV
                     EPSRRIRFDLSEPSTWSALPPDADLIWTFPATPLEQVQAFARHSCRPPGRLVVLGSTS
                     AYDREGGPVDDLPPWIDEAHPINTGLPRVQGEEYLRTHHEAIILRVAGIYGPNRNPVE
                     WIRQGRVGPTNKFVNLIHVEDLAHLCLLALQRAQAGDTYNVSDGHPRRWADICAEVSG
                     RWGVVSARQADRSESGKRIRNHKILTHVGYTLRHPEFYDALQSIEADRRPPIRL"
     gene            2586..3728
                     /locus_tag="PK224_20120"
     CDS             2586..3728
                     /locus_tag="PK224_20120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRB18112.1"
                     /translation="MGPFSARQEETRESSRQSADDPSHRRQRLMVGAAGLLACLFFIL
                     DLQLPLGVANAVLYSAVVFLSAASQYRWLPIGTAITCSLLTIMAAPFSPRVPGLPAWF
                     EWGNHLFSLFGIWTPVMFIYQRRRTELLLKEVNERLEQGVEARTADLAASRLALERSE
                     EQLHTLTGRILTAQEEERRRIAQDLHDDVNQRLALLMLDLQAVDRQIASAPAEAQEGV
                     RNALKGLEELSDDVRFMAYRFHPSILDDLGLKAALQRLLDDFSSRTGVKALFVHQPLD
                     HQLDKTTSTAVYRVIQESLSNIARHAKATRVEVEVTVEDEGLVVVVRDDGRGFDQLAI
                     DRAEGGLGLLNMRERLLAVQGSCEVESNPGRGTTVSMYVPLARVAT"
     gene            3725..4369
                     /locus_tag="PK224_20125"
     CDS             3725..4369
                     /locus_tag="PK224_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRB18113.1"
                     /translation="MTLPRVLLADDHTLVLEGFRRLLDDQCELVGTVGDGRALLEAVP
                     ELKPDIVILDISMPVLNGIDAARVLKVKFPQVKVVFITMHADPAYVRAAFEAGASAYL
                     LKRCVGEELGQAIRAVRSGNFYVTPLVTKDVVESMLRGIDGGAPSGPELTTRQREVLQ
                     LLAEGHTVKDIAGTLKISPRTVEFHKGQIMEQLNLHTTVELVKYALAQGLTSQT"
     gene            4536..4955
                     /locus_tag="PK224_20130"
     CDS             4536..4955
                     /locus_tag="PK224_20130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB18114.1"
                     /translation="MSRPRVLIGDSSRAMLAFLEILLEPQFEVVSSETEAEAIVLTAK
                     KIAPELVILDFSLSFLEGLGAARQLYETMPNSKVVFFSSHEDPVYVTAAFEAGARGYL
                     VKHLTVDLGHYLGRVLQGERVCCPDGLHTRVARTKDT"
     gene            5005..5205
                     /locus_tag="PK224_20135"
     CDS             5005..5205
                     /locus_tag="PK224_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18115.1"
                     /translation="MVRVVPMCELCRRVRDEGASTWGTNSWVDFPSYLARHVVPPSQV
                     RFSRDYCSECRLSYDILKTYGG"
     gene            5424..>6146
                     /locus_tag="PK224_20140"
     CDS             5424..>6146
                     /locus_tag="PK224_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRB18116.1"
                     /translation="MSALPFHPIVAEWFTTRFASATNVQMQAWPAIQSGRDVLISAPT
                     GSGKTLAAFLSCIDTLFQQAVRCELRDETQVLYVSPLKALSNDVQKNLQQPLSEIGQA
                     ALAFGLLLPELRVVVRTGDTPMTERQQMLRRPPHILITTPESLFILLTAEKSRTMLKT
                     VRTVIVDEIHAVAPNKRGAHLALSLERVAALTGAEVQRIGLSATQRPIETIAEFLVGN
                     RNPSIVNGHTSCEAVSHKPLAIS"
BASE COUNT         1070 a         1715 c         1982 g         1379 t
ORIGIN      
        1 catgaatcgt ccgggctagt ataacgaaac cggcggggat tcagccatgg gattgtccgc
       61 agctcccgcc tgcgatctac actgcgttgc gccagtttca cctcttgaaa gccggtggta
      121 tactccggcg ccgatcacgc cccggaggac ctgtggacgc agacgaatca accggaccga
      181 catcgcagat ccccgcggct ccgttgccct gcgtcggggc gcctcctcct ccgcatgctt
      241 acgatcccgg cttctcccgt gccatcacct ggttgtcggc ggtcgttctc agtgcgtcaa
      301 ttgcggtggt cgcctggtta tcgttcgatg tgccgcgtgt ggatcgggtg ccggatgccg
      361 agcgagcgct cagtcatatg gtcgggcgct tgatggatca ggcagacggg ctcaagaccc
      421 tgccggtctg ggaacagttt ctctatgaag ccacgatggg cagcgatgcg aacgatcgtg
      481 agcaggcgat tgaatggtac cgtgaactca ccgaagaatc gtccgacccc tctgtcgatt
      541 tgcacctggc gatcctggag gccgagtcgg ggtaccttcc ggctgtgcag cagaaaatgg
      601 ctcgatgggt tcggcagggt gaaccgtacc cgacctttgc caggctcctg caggccgggt
      661 acgtggatcc tcgcgtgcct cccgcctctg gcttcgagct gcaggcctat ctggcggagc
      721 agtccgtgtc gggctggttc tacagccgtc tggccactcg aatcgccgaa cgagcaggag
      781 accggccctt gctggtgacg attgagacct cgttgcagca gcgggtggaa gcattgttgt
      841 ggcgctcccg ggcgtttgcc ttactcgaat tgacgctcat gatcgtgggg ctgttcttgc
      901 tggtgttgtg ggtccggcgg gggcaaggca cggcgatgtt tcgcgtgggc tccgcggagc
      961 ttccgcccct ctgggccggc cgattgggcg caggggtgtt actgcgcggg ggggcggtgg
     1021 gtgctctgtt gaccgtggcg tttctctatg tcgccggcga ttatccatcg ttgcgggtcg
     1081 tcgcggtgcc gttgtcgaat ctgcccctgc tggccttggc ctactaccat ctgcttcggc
     1141 ctcagcggca gacgttctgg cgaggcttcg ggctacgcat cgaaccgcga cacttgggac
     1201 aattggggct ggcggtgttg gcggtggtag cggctggcct cgtgggcgaa tgggtgctgg
     1261 gcaggattgc ggaaccgttg aatttgatca gtcactggac cgagtggttc gatgcggatc
     1321 tcgtgtgggg atcgtcaccg accctcatgg tcagcctgat ggaatacgtg ttctttgcgc
     1381 ccgtctttga agaactggcc ttccgcggct tgttgttcgg tgtattccgt cgccggtttc
     1441 agtggggggt ggccgccatg ctgagcgcgg cgttgtttgc gctcgcccat gggtacgggt
     1501 tgatcggatt cctcagcgtc ttctggagcg gggtgatttg ggcctgggcc tatgaacgga
     1561 ccggcagtct ctggccgggg atgatcgggc acgccatcaa caatctgctg gtttgtctga
     1621 gtgtgatggc cttgttacgg gcctgagcac cggtggtccg atgcctcaca gcctgatcgg
     1681 gggccgtctg tccgcttcga tcgactggag tgcgtcatag aattccgggt gccggagcgt
     1741 ataccccacg tgtgtcagga tcttgtgatt ccggatgcgt ttacctgatt cgctgcgatc
     1801 cgcctgtcga gcactcacca ctccccacct gccggagact tccgcgcaaa tatcggccca
     1861 gcggcgcggg tgtccatcgc tcacattata ggtgtctccg gcctgtgccc gttgcagcgc
     1921 caggaggcaa agatgcgcca gatcctcaac atggatgagg ttgacgaact tgttcgtcgg
     1981 accgacacgt ccttgtcgga tccattccac ggggtttcga ttcgggccgt agataccggc
     2041 gacgcgtaga ataatcgctt cgtgatgcgt acgcaggtat tcctcgccct gcacgcgggg
     2101 aaggccggta ttgatgggat gggcttcgtc gatccaagga ggaaggtcat ccacggggcc
     2161 gccttcccgg tcatacgcag aggtgctgcc cagcaccacc agccttccgg gagggcgaca
     2221 agagtgtcgg gcaaatgcct ggacctgttc gagcggtgtg gcgggaaatg tccagatcaa
     2281 gtctgcgtca ggaggaagtg cggaccaggt gctcggctct gacaggtcga atcgaatgcg
     2341 tcttgacggc tcgacatcat tcaaatggcg atcggggtga cggctgctgg ccaggatggg
     2401 caaggattgt tgctcggcca atttgtaaat ccagcggccg gtatacccgg atcccagaat
     2461 gacgagagcg cgcggcgtgg tcatcgatgc atcatacctg aacggctgat cggtttactg
     2521 cggtcgactc cggcttattc tggttctggt ctccggcgag cgacgcatgc taagatcctg
     2581 cccctatggg acccttcagc gccaggcagg aggagacgag ggaatcgagc aggcaatcgg
     2641 ccgatgaccc gtcgcatcgg cgacaacgtc tcatggttgg ggcggcgggc ctgctggctt
     2701 gtctcttttt cattctggat ctccagttgc cgctcggagt ggcgaacgcg gtgctgtaca
     2761 gcgccgtcgt ctttctgtct gcggccagcc agtaccgatg gctgccgatc ggcacagcca
     2821 tcacctgctc gctgttgacc atcatggccg ctccgttcag cccccgtgtg ccgggccttc
     2881 cggcatggtt tgagtggggc aatcacctgt tcagtctgtt cggcatctgg actccggtca
     2941 tgtttatcta tcagcgacgg cgcacggagt tattgctgaa ggaagtcaac gagcggcttg
     3001 agcaaggcgt ggaggcgcgc acggcggacc tggccgcgag ccgtctggcc ttggagcgaa
     3061 gcgaagaaca gctgcatacg ttgaccggtc ggattctgac ggcgcaggaa gaggagcgcc
     3121 ggcggatcgc gcaggattta catgatgacg tcaatcaacg gctggccttg ctgatgttgg
     3181 atttgcaggc agtcgaccga caaatcgcgt ccgctccggc agaagctcag gagggtgtcc
     3241 gcaacgcctt gaaggggctg gaggagctgt cggacgatgt gcggtttatg gcgtatcgat
     3301 tccatccctc catcctggac gatctggggt tgaaagcggc cttgcagcgg ttgctggatg
     3361 acttttcgag ccgcacaggg gtgaaggcgc tgtttgtgca ccaaccgctc gatcaccagc
     3421 tcgacaaaac cacatcgacc gcggtgtacc gggtgatcca ggaaagcctg tcgaatatcg
     3481 cgcgacatgc caaggccacc cgggtggagg tggaggtgac ggtcgaagac gaggggctgg
     3541 tcgtggtggt gcgtgacgac ggcagagggt tcgatcagct ggcgatcgat cgggcggagg
     3601 gcggcttggg gttgttgaac atgcgggaac gtttgctggc ggtgcaggga tcctgcgagg
     3661 tggaatctaa tccggggcgg ggaacgacgg tgtcgatgta tgtgccgtta gcacgggtgg
     3721 cgacatgact ctgccgcgcg tgctactggc ggatgatcac acgctggtgt tggaagggtt
     3781 tcggcggctg ctggatgatc agtgtgaact ggtcgggacc gtcggagacg ggcgtgccct
     3841 gctggaggcc gttccggagt tgaagccgga tatcgtgatt ctcgacatct cgatgccggt
     3901 gctgaacggg atcgatgcgg cgcgtgtcct gaaggtcaag tttccccagg tcaaagtggt
     3961 ctttatcacg atgcatgccg atccggctta tgtgcgggcg gctttcgagg ccggggcctc
     4021 cgcctacctg ctcaaacgct gcgtgggcga agaactgggg caggcgatac gggctgtgcg
     4081 gagcgggaac ttctatgtga cccctctagt gaccaaggat gtggtggaaa gcatgttgcg
     4141 tgggatcgat ggcggggcgc cgtcgggtcc ggaactgacc acccgtcagc gcgaagtgct
     4201 gcaattgctg gccgaaggcc atacagtaaa ggatattgcc ggcaccctca aaatctcccc
     4261 gcgcaccgtt gagttccata aaggccagat catggaacag ctcaatcttc acaccaccgt
     4321 cgaactcgtc aaatatgcct tagcccaggg attaaccagc cagacatagc tgccttccta
     4381 ccgttgcctc tccttcggct cttgaattac tagtctgaat gcaggtattt tttagctgat
     4441 atgactcgcg ttgttacgcg tgtggttttc ccttcacttg cctactatac gcctccattt
     4501 tctaagcagt gactcgtagg aatatgagaa agaagatgtc acgtccacgt gtcctcatcg
     4561 gcgattcctc gcgggctatg ctggcctttc tggagattct gttggaacct caattcgagg
     4621 tcgtgtcttc cgaaactgaa gcggaagcca ttgtattgac tgcaaaaaaa atcgcaccgg
     4681 agttggtcat tctggatttc tccctctcgt ttctcgaagg gcttggggct gcccggcagc
     4741 tgtatgagac aatgccgaac agcaaggtcg tgtttttttc ttcgcatgaa gaccctgtct
     4801 acgtcacggc cgcgttcgag gcaggcgcca ggggatatct cgtcaagcat ctcacggtcg
     4861 atctcgggca ttatctcggt cgggtgctgc aaggtgaacg ggtctgctgc ccggatggtc
     4921 ttcacacgcg ggtggctcgc acgaaagaca cctgaccgct tgctgtccgg ccatgcgatg
     4981 gattcgcgca aaaggagacc gacgatggtt cgagtcgtac cgatgtgtga gttgtgccgc
     5041 cgggttcgtg atgagggggc ctcgacctgg gggaccaaca gctgggtcga ttttcccagt
     5101 tacctggcgc gacatgtcgt gccgccctct caggtgcggt tttcaaggga ctactgcagt
     5161 gagtgccggc tctcctacga tattttgaag acctacgggg ggtaatgggg gcgaaagtca
     5221 tcgctcgaaa cattccctcc gaagcgtcta gtgagcatta tcggtcgcgt cgtatcccac
     5281 cagtcctgat cgtcccggtt tctctgttct gaacatcccc attgtcggga tctccgctgc
     5341 ctcgccttcc atttccttaa tccgcatccg tggcggcctg cgcgatgtgt tgtcgtttcc
     5401 tgttgtgcta cgatgattct ctgatgtctg ccctgccgtt tcatcccatc gttgccgagt
     5461 ggttcacgac ccgattcgcc tctgcgacga atgtccagat gcaggcctgg cctgcgattc
     5521 aatccggtcg tgacgtgctg atctcagcgc cgacggggtc ggggaagacc ctcgcggcct
     5581 ttctctcctg catcgatact ctctttcagc aggcggtacg ctgtgagctg cgggatgaga
     5641 cgcaggtgct ctatgtctcg ccgctgaaag cgctgagcaa cgatgtgcag aaaaatctgc
     5701 aacagccgct cagcgagatc gggcaggccg cgttggcttt cggcctgttg ctgccggaac
     5761 tgcgggtggt cgtgcgtacc ggcgacacgc ccatgactga gcggcaacag atgcttcggc
     5821 gtccgccgca tattctgatc acgactcccg aatccctctt tatcctgctg acggcggaaa
     5881 aaagccggac catgttgaag accgtgcgca cggtcatcgt ggatgaaatt catgccgtgg
     5941 cgcccaataa acgaggggcg catctggccc tgtcgctgga gcgggtggcg gcgctcaccg
     6001 gcgccgaggt tcaacgcatc ggcctgtcgg cgacgcagcg gcccatcgaa accatcgcgg
     6061 agtttttggt gggaaataga aacccgtcaa tcgtcaacgg tcacacgtca tgcgaagccg
     6121 tcagtcataa gccattagcc atcagc
//
LOCUS       DAORRQ010000127         6118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_460_length_6118_cov_8.522019, whole genome shotgun sequence.
ACCESSION   DAORRQ010000127 DAORRQ010000000
VERSION     DAORRQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_460_length_6118_cov_8.522019"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PK224_20145"
     CDS             <1..1201
                     /locus_tag="PK224_20145"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRB18117.1"
                     /translation="PWTPPPLKPAPVPERQPQGTAWFRRTVVGMLAFLTITLFTASLA
                     QAVRVKDVATIEGVRENQLIGYGLVVGLDRTGDQVIGGQFTIQAMMSMLNKMGINLVI
                     DPIQLLTRNIASVMVTAKLPPFVKPGMTLDAVVSSMANAKSLQGGTLLSTPLKAPNQQ
                     VFAVAQGPVSIGGFLGGTGGAGGATVTKNHQAAGVVPAGAIVEKELVVDIENWDSVSV
                     LLRHPDFTTAIRTAEAIDGTFGKGTAVPVNAGLVKTSLPATFHGRVVEYIATMEGLDV
                     TVDVAAKVVVNERTGTVVLGEHVRLSTCAISHGNLTISVKNTLNVSQPPAPLIGSTQG
                     QTTVTPDVQTEVTEQESRLIVVDQTVTLGEVVRALNAVGVTPRDLVAILSALRSAGAL
                     QANLEIV"
     gene            1238..1549
                     /locus_tag="PK224_20150"
     CDS             1238..1549
                     /locus_tag="PK224_20150"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HRB18118.1"
                     /translation="MSSQLDLAKMATPQPLGFRGPAEQGPAGDKARAALHKAGQEFEA
                     YFISYLIKNMRETVPKGLLDRKSEQVWYSFYDQEIAKLSTEAGGIGITAFVDAYAEKL
                     P"
     gene            1654..1959
                     /gene="flgM"
                     /locus_tag="PK224_20155"
     CDS             1654..1959
                     /gene="flgM"
                     /locus_tag="PK224_20155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HRB18119.1"
                     /translation="MEISHNGRAADLAKILLGVQDTERTHNKKTASQGTQSQDRVQIS
                     EQAKELQRLRAAAEQPDAERDARVGQIRQSVEGGTYNVDGKKVADAIIRNVLTDAVL"
     gene            2033..2485
                     /locus_tag="PK224_20160"
     CDS             2033..2485
                     /locus_tag="PK224_20160"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HRB18120.1"
                     /translation="MVSTVPQPLSTASDSLLCRMLDKVTAFQALLLEEQDAIRSLSFG
                     QFTSVTMRKTQLLDEMRELEQQRRNEATLSGSYGSPPSLKQQEADLVAAIGQTDRLNR
                     FNAALIGQSLEFLQGTLRIWQRSPQSAALYSSSGAAVAGNGGRVRAKG"
     gene            2497..3891
                     /gene="flgK"
                     /locus_tag="PK224_20165"
     CDS             2497..3891
                     /gene="flgK"
                     /locus_tag="PK224_20165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HRB18121.1"
                     /translation="MSGLNGLFGIGSNALATFQRALSVTGQNIANVSTPGYSRQEITL
                     SESLPENGQPGQIGTGVTATEIRRSVDSFVERQLLSSNERVGQFGASQKALSQIQLVF
                     NDANDQGIAAGLNEFFKAWQDVATNPADLTARTVLLTKADGLTKLLNQADAQLSAQRT
                     SLDGQVQSSINDVNALASKIADLNSQIKLTEISGQHANDLRDQRGRFLNDLAGLVEIS
                     SIEDATGQVTVFVGIGQVLVAEKTAYTLTGVANVANNGLLDVRYDGGTGPNTDITSAI
                     SGGRLKGLIDARDTTAAGLQTSLNTLTSQLVSQVNTQHRLGYGLDGSTTQDFFTASGT
                     AAGTISLALTDRQKIAASSTAAGVPGNNVNALALSNLQTASVAGLGNTTFQGYYSAMA
                     GSFGATLQGATRDLQGQEILHDQLLAHRAEVSGVSMDEELINLLKYQRAFEAASKLIT
                     TSDEMLQTILTLKR"
     gene            3923..4813
                     /gene="flgL"
                     /locus_tag="PK224_20170"
     CDS             3923..4813
                     /gene="flgL"
                     /locus_tag="PK224_20170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HRB18122.1"
                     /translation="MRVADQQMYGILLGNLQRSRLQMLTSQEQISSQKRVANPADDPS
                     AFGQIVLDKSALSQTTQWVRNINFGTSRVNAADQALGQVQNLITRVRELTIQASSDTT
                     SAEGRQSIAKEVRQLQRQLVQLGNTEVAGQAIFGGTKTDVQPFTITSGDTVAYQGNSE
                     TQSIAVGENQTVQILVPGSSIFTGSTTNMFDSLRDLLTALESNNRSGIQAGLGNLDLA
                     TAQISDVQGTVGALANRLQVTHDALDTATLTITKSISDNQDADLATAITQLRLQEVAV
                     QAASETFTKIFDSSLINYLR"
     gene            4887..>5030
                     /gene="csrA"
                     /locus_tag="PK224_20175"
     CDS             4887..>5030
                     /gene="csrA"
                     /locus_tag="PK224_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HRB18123.1"
                     /translation="MLVLTRRRGEGVTIGPDVRIVVLGIKGGQVRLGIEAPPNVQVHR
                     DEVH"
     assembly_gap    5031..5051
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5129..5605
                     /locus_tag="PK224_20180"
     CDS             5129..5605
                     /locus_tag="PK224_20180"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HRB18124.1"
                     /translation="MKCQSTRFGTFEVNDDTLLVFPSGILGFPEWSKYVLLDHDTEAP
                     FKWLQCVEEPQLAFVILDPDFFKPDYQIEVSLDALIEIQKQDSDELSVVTILTIPSDD
                     PTAVTANLRGPLVMNHRTRLCKQLVLSEDWPTRYPLFSAPPAQRPTPTAPLQATAG"
BASE COUNT         1352 a         1791 c         1778 g         1176 t
ORIGIN      
        1 cccctggaca cctccgccgc tgaagccggc gcctgttcct gaaagacagc cacaaggaac
       61 ggcctggttt cgacgcaccg tggtgggcat gctggccttc ctcacgatca cgttatttac
      121 ggcctcatta gcccaggctg ttcgagtcaa ggacgtggcc acgatcgagg gcgtgcgcga
      181 aaaccagctg atcgggtatg gattggtcgt cggtctcgac cggacgggcg atcaggtcat
      241 cggtggacag ttcacgattc aagccatgat gtccatgttg aacaagatgg gcatcaatct
      301 ggtgatcgat cccatccagt tgctcacccg caatatcgcg tcggtgatgg tgacggcgaa
      361 gctcccgccg tttgtgaaac ccggcatgac gctggacgcg gtcgtctctt ccatggccaa
      421 tgcgaaaagt ctccaaggcg gcacgctgct gtcgacgccg ctgaaggcgc cgaaccaaca
      481 ggtgtttgcg gtggctcagg ggccggtctc cattggtgga ttcctcggcg ggaccggcgg
      541 cgccggcggc gcgacggtca ccaagaacca tcaagcggcc ggggtggttc ctgccggcgc
      601 catcgtggaa aaggagttgg tggtagatat cgagaactgg gacagcgtgt ccgtgctgct
      661 gcggcatccc gatttcacca ccgccattcg cacggcggag gcgatcgacg ggacgtttgg
      721 gaagggcacg gctgtcccgg tgaatgcggg cctcgtcaaa acgtcgcttc ccgcgacgtt
      781 tcacggacgg gtcgtggaat acatcgcgac gatggaagga ttggacgtga ccgtcgatgt
      841 cgccgccaaa gtggtggtga atgaacgcac cggtacggtc gtcctcggcg aacatgtccg
      901 gttatccaca tgcgcgatct cgcatggcaa cctcacgatt tccgtcaaga acacgctcaa
      961 cgtgtcacag cctccggctc cgttgatcgg ctcgacccaa gggcaaacga cggtcactcc
     1021 cgatgtgcag acggaagtga ccgaacagga gtcgcgactg atcgtggtcg atcaaacggt
     1081 gacgcttgga gaagtggtac gggcactcaa cgcggtgggc gtgactccgc gagatctcgt
     1141 ggccatccta tcggccttgc gttctgccgg agcacttcaa gcgaatcttg agattgtcta
     1201 agaaagggcc acgcttccaa tggaaataca aaattttatg tccagccaat tggatctggc
     1261 caaaatggcg actccgcagc cgctcggatt ccgcggtccg gccgaacagg ggccggcagg
     1321 ggacaaggcc cgcgcggcgc tccataaagc cgggcaggag tttgaagcgt atttcatctc
     1381 gtatcttatt aaaaatatga gggaaactgt tccgaaggga ctcctggacc ggaaaagcga
     1441 gcaggtctgg tactcctttt acgatcaaga gatagccaaa ttgtccacag aagcaggggg
     1501 gatcggcatt acggcattcg tcgatgccta tgcggaaaaa cttccctaat tcgatcgctt
     1561 ttcctaaagt tcccggccga tcccgccgat agagtactcg actaggggaa aggcctttcg
     1621 gaagaccctg gctatggggg aggagaagca gtcatggaga tctcacataa cggccgagcg
     1681 gcagatctcg cgaaaatcct gttgggagtt caggacactg aacggacaca caataagaag
     1741 accgcgtctc aagggacgca gtcacaggat cgtgtccaga tttccgaaca ggccaaggaa
     1801 ctccagcgtc tgagggcggc agcggaacag ccggatgcgg agcgtgatgc cagagtcggt
     1861 caaattcgtc agtcggtcga agggggcacg tacaacgtgg acggtaagaa ggttgcggat
     1921 gccatcattc gtaacgtcct gaccgacgca gttctgtaac gcccttcgcg tacggctcga
     1981 ctcttcgatc acgcggcctc acatggcgga accgttcgtt tgaaggggag acatggtgtc
     2041 gaccgtaccg cagccacttt ccacagcgtc cgactccctt ctttgccgca tgctcgacaa
     2101 ggtcacggcc tttcaggccc tgctgcttga ggagcaggac gccattcgct ccctttcgtt
     2161 tggacaattc acctccgtga ccatgcgaaa aactcaacta ttggatgaga tgcgtgaatt
     2221 ggaacagcaa cgccggaacg aagcaacctt gtctggttcg tatggctcgc cgccctcgct
     2281 caagcaacag gaggctgatc tcgtcgcggc gatcgggcag acggatcggc tgaaccggtt
     2341 caacgccgcc ctcatcgggc agtcgctcga gttcttgcaa ggcacgctcc ggatctggca
     2401 gcgctcaccg cagtcggcgg cactctattc gtcctcgggt gccgcggtcg caggaaacgg
     2461 cggcagagtg agagccaagg gataacgggt ggtcgtatgt cgggactcaa tggattattc
     2521 ggcatcggtt ccaatgcgct ggccaccttc cagcgcgccc tgtccgtcac aggccagaat
     2581 atcgcgaacg tcagcacccc gggctactcc cggcaagaga tcaccctcag cgaaagcctg
     2641 cccgagaatg gtcagccggg tcagatcgga accggcgtca cggccactga aatccgccgc
     2701 tccgtcgata gctttgtgga acgtcaattg ttaagttcca acgagcgggt ggggcagttc
     2761 ggagcttcac agaaagcgct ctcgcaaatt caattggtct tcaacgatgc gaacgaccaa
     2821 ggcatcgcgg cgggactcaa tgaattcttc aaggcctggc aggatgtcgc caccaatcct
     2881 gcagatctga cggcccgtac ggtgctgctg acaaaagccg atggattgac caaactcttg
     2941 aaccaggccg acgcacaact gtcggctcaa cgcacctccc tcgatggcca ggtgcagagc
     3001 agcattaacg acgtcaatgc cctggcgagc aagattgccg atctcaacag ccagatcaag
     3061 ctgacagaga tcagcggcca gcacgccaat gatttacgcg atcaacgcgg tcgctttctc
     3121 aacgaccttg ccggcctggt cgagatctcc tcgattgagg acgcgaccgg acaggtgacg
     3181 gtattcgtgg gaattgggca ggtgctggtc gctgagaaga cagcctacac actgaccggt
     3241 gtcgcgaacg tcgccaataa cgggctcctg gatgtgcgct acgatggggg aaccggaccc
     3301 aatacggata tcacgtcggc gatcagcggc ggccgcctca agggtttgat cgacgcgcgt
     3361 gacacgaccg cagccggtct gcagacatcg ctcaataccc tcacctctca actcgtctcg
     3421 caggtcaaca cccagcatcg cctggggtac ggtctggatg gatccacgac gcaggacttc
     3481 ttcacggcct cagggaccgc ggccggcacc atcagcctgg ccctgaccga tcgacagaag
     3541 atcgcggcct catcgacggc cgccggcgtg cccggcaaca atgtgaatgc cctggcgtta
     3601 agcaatctgc aaacggcctc ggtggcggga ttgggcaata cgacctttca gggatactac
     3661 agcgcgatgg ccggcagctt cggagcgacc ctgcagggag ccacacgcga tctgcaaggg
     3721 caggagatcc tgcacgacca gctgctggcg caccgggcgg aagtgtccgg agtatcgatg
     3781 gatgaagagc tcatcaatct gttgaagtat cagcgcgcat ttgaggcagc ctcgaaactc
     3841 attaccacca gcgatgagat gctgcagacg attctgaccc tgaagcggta gccgccttct
     3901 catctcacag ggagcagcca gtatgagagt cgccgaccaa caaatgtacg gaatcctgct
     3961 cggcaatctt caacgctccc ggttgcagat gctcacctcg caggagcaaa tttccagcca
     4021 aaaacgggtc gccaatccag cggacgatcc gagcgcattc ggacagatcg tgctcgacaa
     4081 gtccgcgctt tcgcagacga cgcagtgggt tcgcaatatc aatttcggga cctcacgtgt
     4141 gaatgccgcc gatcaggcct tgggacaggt ccagaacctg atcaccagag tgcgggagct
     4201 gaccattcag gccagcagcg acacgacgtc cgcagaaggg cgtcagagca tcgccaaaga
     4261 ggtccggcag cttcagcgcc agctggttca actcggcaac acggaagtgg cggggcaggc
     4321 gatcttcggc ggtacgaaga cggatgtgca gcccttcacg atcacatcag gagatacggt
     4381 cgcctatcag ggcaacagcg agacccagtc catcgcggtc ggtgaaaatc aaaccgtgca
     4441 gatcctcgtg ccgggaagca gcatcttcac cggatcgacg accaacatgt tcgattcgtt
     4501 gcgggacctc ctcacggcgc tggaaagcaa caatcgctcc ggtattcaag ccggactggg
     4561 aaatctggat ttggcgacgg ctcaaatcag cgatgtacag ggcaccgtcg gcgctctcgc
     4621 caaccgcctc caggtcacgc acgacgcctt ggacacggcc accttgacca tcacaaaatc
     4681 catctcagac aatcaggatg cggaccttgc cacggccatt acgcaactcc gcctccagga
     4741 agtcgccgtg caggccgcca gtgaaacctt caccaaaatt ttcgactcgt cgttgatcaa
     4801 ctatctccgc tgatcgacgc tcaagttcac cttcatcgaa accgatatat ccatgtactt
     4861 gtattcacac gccaaggaag gcttgtatgc tggtcttaac acgacgccgg ggagaaggcg
     4921 tgaccatcgg tcccgatgtt cgcatcgtgg tgctcggcat caaaggcggc caagtccggc
     4981 tcgggatcga agcaccgccc aatgtgcagg tgcatcgcga tgaagtgcat nnnnnnnnnn
     5041 nnnnnnnnnn nggccagggg ttattccatt ggaagcgttt cgtcaattgt cgaacaaaac
     5101 cgggcgtcgt ggaggttgag gaaccgtcat gaagtgtcag tcgaccaggt tcgggacttt
     5161 tgaggtaaat gacgacacgc tcctggtgtt tccttccggt attctgggct ttccggaatg
     5221 gagcaagtac gtgctgctcg atcacgatac cgaggccccg ttcaagtggc tgcaatgtgt
     5281 cgaagaaccc cagctggcct tcgtcatcct ggatcctgac ttcttcaagc ccgattatca
     5341 gatcgaggtg tcgttggatg cgctcatcga aattcaaaag caggacagcg atgaactgtc
     5401 cgtcgtcacg atcttgacca ttccttcaga tgatcccact gccgtgacgg ccaatttgcg
     5461 gggaccgctg gtcatgaatc accggaccag gctctgcaag caattagtcc tctcggagga
     5521 ttggccgact cgatatcctc tcttttccgc tccccccgca caacgcccca ctccaacggc
     5581 tccgcttcag gcgacagccg gctaaacacg catcactcat cgcgtaccgg gctcagcgtg
     5641 cgccgtgtcg gtatgacaat aacccgctgg atgcgcaaaa aggccgtctt catagtccgt
     5701 cgttcgtgaa acgtcgttcg tctctcgtct gcagaaaaga gcttatggca tagagctcat
     5761 agcgtatggc cagaaatcga gagtgggcat ctctactctc agctgtatgc caccagcgat
     5821 acgctctgac cgcgacgaga tacgcttcac gattcacgga tgtcgaggac gccgctggcg
     5881 gactttttcc gcatcctgct aaaaaaagag cgttgcggct ttcccatgca ctgtgaatag
     5941 actagcgcga ctgagccggg tcggctggtg tgatctggcg ctccagcacc gcaaccagtt
     6001 gctgaaccgt acgttcgagc tgggcgaata gggcaggggc ttcggcgagc gtcccgagac
     6061 gtccgttggc ctccagctga cttgccagga gcaccacctc cggtgcgcac aaattacc
//
LOCUS       DAORRQ010000128         6074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_463_length_6074_cov_21.781691, whole genome shotgun sequence.
ACCESSION   DAORRQ010000128 DAORRQ010000000
VERSION     DAORRQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_463_length_6074_cov_21.781691"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="PK224_20185"
     CDS             complement(<1..1300)
                     /locus_tag="PK224_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18125.1"
                     /translation="MAGDREESKIPITTVVGLAMALATLWLVREPLLSSRPEVEPAIG
                     REAELIQARLWQDPIEAAESHQQSDRTEQPRHNHSFSSIHFESREDPGVHIGVLIIIT
                     EGSPYAENHEKRLRDRYAALSALGVACLVPADEEHIGYFEVGQSLVPFEWYKARKTRQ
                     CHQDKDHKQHYDRVLLVWATDDSLGERPLNRLNEISGLLASQYWADVCGGRHSVECMA
                     KLEFKIIGPPSSSMLRVMLQNARDQAAPKWWPNIGSLEGPTSFKIALYSPWATASPEL
                     LLQGIDSNGQANTHRCEGGQSCRKNLAAQLANAGVVLEHTVEGDEEMSLALVGELGRR
                     QVKIGEDPIALIGEWDTFYGRALPIVFRAAACVVASENITDELNKVESMDEGAERNRR
                     DAVLYKRFRECQSISRAIRSQIEHPERWDALEMRISRYSYL"
     gene            complement(1550..3025)
                     /locus_tag="PK224_20190"
     CDS             complement(1550..3025)
                     /locus_tag="PK224_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HRB18126.1"
                     /translation="MTVHSLETLTFDNSYARLPEVFYAKVTPTPFSAAPFLISANRAA
                     MELLDLDPAEAARPEFAGVFGGSLLIPGMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     VTNGRGERWDLHLKGAGMTPFSRDGDGRSVLRSAIREYLCCEAMHGLGIPTTRALCLV
                     GSDDKVYREQIETGATIVRMAPSHVRFGTFEIFYYRKQHEHLQRLADYTIEMHFPELA
                     QAVDKYARFFAEVVQRTAKLIAHWQAVGWSHGVLNTDNMSILGLTLDYGPYGFMDDYD
                     PGFICNHSDYNGRYAFNQQPYIGLWNLSCLAQTLLPFAPKEELKAALDGYQTSVDRHY
                     HNHMRAKLGLVEDRAEDEALLQELKSLMVGSRVDYTIFWRELSMFSSDAGAKNERLRE
                     HFLNPERFDTWAVQYRERLQSEQSRDEERRIRMDRVNPKYVLRNYLAQGAIEKAQQKD
                     YSEVERLLTLLQQPYTEQPGMNSYAAAPPNWGKHLSVSCSS"
     gene            complement(3046..3471)
                     /locus_tag="PK224_20195"
     CDS             complement(3046..3471)
                     /locus_tag="PK224_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HRB18127.1"
                     /translation="MAVTIIGGALSRSTIQKDLLPVASKGEKEAAVRTAIIKFEQEFL
                     GRGPDEVRVFIVRDMLVVRLKGVLTPAERQLAKTAEGIDMVKRLRQNLIAQGRERLCE
                     QVMDLIGAKVTALFTDIDTVVGERIFVFTLESDIEANFR"
     gene            3814..5508
                     /locus_tag="PK224_20200"
     CDS             3814..5508
                     /locus_tag="PK224_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRB18128.1"
                     /translation="MSFLALVPLLPLLTAFIVMTGDRAEQDQNARAGLLPVAAAFLGS
                     LIALVAVTSGDPVTIQFYDTASVANLAFPIGFYIDRLSAVMMVLITGVTMLIYRYSMG
                     YMYQDRGYRRYLGMLGITTAVLLCMVSSANLVMLFVFWQILSWLLFLLAHNHGHAATL
                     SGAANTFTMLRLSDAAFLAGIVLAYSLYGTFEFQTLFARAADTPVTLSLWPDLGWEMN
                     GATAVTLLIFVGAMGKSAQFPIHTWLPRSLYAPTPIHALLHAGIINAGGFLLNRLAPL
                     YGLSPTTLHVVFVIGMLTAILGATMMLTQNDIKKTLGFSTIGQMGYMIMECGLGAFSL
                     AVFHLIAHGLFKGTVFLNCGNVIHKARQEPSFPPIDREAEESEFSNLTWSTGFLTTLL
                     LPLVILLVTHGVLRIPLIDSQGTVIFLFFIWVTSSQAILSLTRIRAVASWKVSAAMLV
                     TLVIVVFTYLFAVESFTHFLYPNPEVVASYFKAAALPGRLFDSLVVGTTVLTILSWVY
                     LYAHAHGRTVKIPGWIDVFLVRLYVLFMNRLYLDQLYLKFGRIVTLVAHHLEKRLS"
     gene            5564..>6074
                     /locus_tag="PK224_20205"
     CDS             5564..>6074
                     /locus_tag="PK224_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18129.1"
                     /translation="MIKTWMTPWLLMAVPFLGATLSLLLRTSLQRMKTSALLTTVASM
                     LVVIGTPWALGDTLAGVPFLCLLPLTAFLSLLGQPLHRDNTSAWTTTLVLLGLGLGIL
                     TAQAPAHDVMLLILPGLLCILLYRYQSGATPETWRGLSAYGLSMMSAALALVLPTPAS
                     TVALLVTCAT"
BASE COUNT         1131 a         1895 c         1681 g         1367 t
ORIGIN      
        1 ggagatagga atagcgggag atcctcatct caagcgcatc ccaacgttct gggtgttcta
       61 tctgggaacg aatggcccga gagatagatt ggcactccct aaaccgttta tagaggacag
      121 cgtcacgccg attcctttct gccccctcgt ccatactctc gactttattt agttcatcgg
      181 taatattctc actagcaacc acacaagctg ccgctctgaa aacaattgga agcgctcggc
      241 catagaacgt gtcccattct ccgataagtg caatgggatc ttcgcctatt ttgacctgtc
      301 gccttccaag ttctcccacc aaggcgagag acatctcttc atcgccctcg acggtatgct
      361 cgaggacaac acctgcatta gccagttggg cggccaaatt tttcctgcac gattgacccc
      421 cttcgcacct atgggtattg gcttgcccat tcgaatcaat cccttgcaaa agaagttcag
      481 gagaggctgt ggcccaaggt gaataaaggg cgatcttgaa cgacgtaggg ccctcaagag
      541 atcctatgtt cggccaccat ttaggcgcag cctgatctcg tgcgttctgt agcataaccc
      601 tgagcatgct ggagctaggt gggccgataa tcttaaactc gagcttggcc atacactcta
      661 cagaatgccg ccctccacag acatcggccc aatactggct cgcaagcaac cctgaaattt
      721 cattcagtct attcaacggc ctctctccca gactgtcatc cgtcgcccag actaggagaa
      781 cccgatcgta gtgctgcttg tgatccttgt cctgatgaca ttgtctagtc tttctcgcct
      841 tataccactc gaacggaacg aggctctgcc caacctcgaa gtacccgatg tgctcttcgt
      901 cggccggcac aaggcacgcc acaccgagag cggagagtgc tgcatagcgg tcgcgcaacc
      961 gcttctcatg gttctcggca tatggacttc cctcggtaat gatgatgaga accccgatat
     1021 gcaccccggg gtcttccctg ctctcaaaat gaatactgga gaaactgtgg ttgtggcgtg
     1081 gctgttcggt acggtcgctt tgctgatgag attctgccgc ctctattgga tcctgccaga
     1141 gtcgcgcttg aatcagttcc gcttcccggc ctatggctgg ttcaacctca gggcgtgagc
     1201 tgagaagagg ctcacgcacc agccagagcg tagctaaggc catggccagt ccgacgactg
     1261 ttgtgatggg aatttttgat tcttcccgat cccctgccat aagagcccct cacatccctg
     1321 tggctggact catggatgta gcacccactg gtcgatccag caaaagctat gcctgcatct
     1381 aagctccgct gaaccacaac tctccagggc tagaggatga actaataaag gattactggt
     1441 gcgcgcgatg ctcagccagc gatgagggaa agagtatctt cctcgatacg agatgtatct
     1501 attcagataa gtacaagagg ctaggtcttt gctcgatgac gtggtgatgc tacgacgagc
     1561 agctgacgct gaggtgttta ccccagttcg ggggcgcggc ggcgtaggaa ttcatgccgg
     1621 gttgttctgt gtagggctgc tgcagcaggg tcagcagccg ttcgacctcc gaatagtctt
     1681 tctgctgggc cttctcaatg gccccttggg cgagatagtt gcgcaggacg tatttgggat
     1741 tcacccgatc catacgaatc cgtcgctctt cgtcgcgact ctgctcgctc tgcagccgtt
     1801 cgcgatactg aacggcccag gtatcgaacc gctccggatt caggaaatgt tcccgcagcc
     1861 gttcgttctt cgcgcccgcg tcggatgaaa acatgctcaa ttcacgccag aagatcgtgt
     1921 aatcgacccg gctgccgacc atgagcgatt tcagttcttg cagcagggcc tcatcttccg
     1981 cgcggtcctc caccaacccc aatttggcac gcatgtggtt gtgatagtgc cgatccacgc
     2041 tggtctgata accatcgagc gcggctttca gctcttcctt gggcgcaaac ggcagcaggg
     2101 tttgcgccag gcaactcagg ttccagagtc cgatgtaggg ctgctggttg aaggcatagc
     2161 gcccgttata gtccgagtgg ttgcagatga agccggggtc gtagtcatcc atgaaaccgt
     2221 atgggccata gtccagcgtg aggcccagga tcgacatatt gtcggtattg agcacgccat
     2281 gcgaccagcc gaccgcttgc cagtgcgcga tcagtttcgc cgtgcgctgg accacctcgg
     2341 caaaaaaacg ggcatatttg tcgactgcct gcgcaagttc agggaaatgc atctcgatgg
     2401 tgtaatcggc cagccgctgc agatgctcgt gctgcttccg gtagtagaaa atttcgaacg
     2461 tgccgaagcg cacatgcgac ggcgccatcc ggacgatcgt cgccccggtt tcgatctgtt
     2521 cgcgatagac cttgtcgtcg ctgccgacca ggcacagcgc acgcgtcgtc gggatgccaa
     2581 gtccatgcat cgcctcgcaa cagagatatt cacggatggc cgaacggagc accgaacgcc
     2641 catccccgtc gcgcgagaag ggcgtcatgc ccgcgccttt gaggtgcaga tcccagcgtt
     2701 ccccgcggcc gttcgtcact tcccccagaa ggatcgcccg gccatcgccg agttgcggca
     2761 catagacacc gaactgatgg ccggagtaga gcatggccag cggttccatg ccgggaatca
     2821 gcaggctccc gccgaacacc ccggcgaact ccggtcgcgc cgcttccgcc gggtcgagat
     2881 cgagcaactc catcgcggcc cggttcgcgc tgatgagaaa cggcgcggcc gagaagggcg
     2941 tcggggtcac cttggcgtag aagacctccg gcagacgcgc gtaactgtta tcgaacgtga
     3001 gggtttcgag actgtggact gtcatcgcgc ttcctgacct tggggctacc ggaaattggc
     3061 ttcgatatcg gattccaacg tgaagacaaa aatgcgctcc ccgaccacgg tgtcgatgtc
     3121 ggtaaacagc gccgtcactt tcgcaccgat cagatccatc acctgttcac agaggcgctc
     3181 ccgaccttgc gcgatgaggt tctgccgaag tcgtttcacc atatcgatgc cttcggccgt
     3241 tttggccagc tggcgttctg cgggtgtcag cacccccttc aaccgcacga caagcatgtc
     3301 ccgcacaatg aagacccgaa cttcatccgg gccgcggccc agaaattcct gctcgaactt
     3361 gatgatcgcc gtccggacgg cagcttcctt ctctcccttg ctcgccacag gcaatagatc
     3421 cttctgaatg gttgatcgag acaacgcgcc gccgattata gtcaccgcca tgtgcagact
     3481 ccaaccccct tccaccgctg tcggcaaacg atccggcacg acgccggcga ccaggcccat
     3541 gggccttgcc agcaagccct gcggcgccga ctttttactt gcaattacaa aggcgcgcgg
     3601 ctacatctac cgtagttttc cggtggtgcc gccgagaggc cgtgaatcgc gcgtggcccg
     3661 gacccaccac atggatgtga tgagacaggc agtagggtct tgccggtgtt tccctccagg
     3721 gaaaagccgg ggacagtgca ggccagtcgt tggttccttg tttcaaggac cgtcggctgg
     3781 cctttttctt ttcatgagtg ccatggggtc ggcatgtcgt ttctagcgct cgtcccgctg
     3841 ttgccgttgt tgacggcctt catcgtgatg accggcgacc gtgcggagca ggaccagaat
     3901 gccagagccg ggctgctccc cgtcgcggcg gcatttctcg ggtccctcat cgccctggtt
     3961 gcggtcacct ccggagatcc ggtcaccatc cagttctacg atacggcctc cgtcgccaat
     4021 cttgccttcc ccatcgggtt ctatatcgac cggctcagcg cggtcatgat ggttctcatc
     4081 accggcgtga ccatgctcat ctaccgctat tccatgggtt acatgtatca ggatcgcgga
     4141 tatcgccggt atctcgggat gctcggtatc acgaccgcgg tgctcctctg catggtgtcc
     4201 agcgccaacc tggtcatgtt atttgtcttc tggcagatcc tctcgtggct gttgtttctg
     4261 cttgcacaca accacgggca tgccgccaca ctgtccggcg cggcgaacac gtttacgatg
     4321 ttgcggctca gcgatgcggc gtttctggcc ggtatcgtcc tggcctattc gctgtacggc
     4381 acattcgagt tccagacatt gtttgcccgc gccgcggaca cgcccgtcac cctgtccctt
     4441 tggccggacc tcggctggga gatgaacggc gcgacggcgg tgaccctact gattttcgtc
     4501 ggcgccatgg gcaagtcggc acaatttccg atccacacct ggctgccgcg ctccctctac
     4561 gcgccgacgc ccatccacgc actgctgcac gcgggcatca tcaacgccgg gggattcctg
     4621 ctgaatcgtc tggccccgct ctacggtctg agcccgacca ccctgcatgt ggtgttcgtc
     4681 atcggcatgc tcacggcgat tctgggagcc accatgatgt tgacgcagaa cgacatcaag
     4741 aagacgctcg gcttttcgac gatcggccag atgggctata tgatcatgga atgcgggctg
     4801 ggcgcgtttt ccctcgccgt gttccatctg atcgcccacg gtctgttcaa gggcacggtg
     4861 ttcctgaatt gcgggaatgt gatccacaag gctcgccagg aaccctcgtt tccgccaatc
     4921 gaccgcgagg cggaggaaag cgaattctcc aatctcacct ggtccaccgg attcctgacg
     4981 acgctgctct tgccgctcgt gatcctgctc gtcacgcacg gtgtcttgcg aattccgctg
     5041 atcgattcac aaggcacggt gattttcctg ttcttcatct gggttacctc ctcgcaggcg
     5101 attctctccc tgacacgtat tcgcgccgtg gcctcctgga aggtgtccgc cgccatgctg
     5161 gtcacgctcg tcatcgtcgt cttcacatac ctctttgcgg tggagagctt tacgcatttt
     5221 ctgtatccga atccggaggt ggtggcgtcg tatttcaagg ccgcggcgct gccgggccgg
     5281 ctcttcgaca gtctggtggt cggcacgacc gtgctgacga tcctcagctg ggtctatctc
     5341 tatgcgcatg cgcatgggcg cacggtcaaa attcccggtt ggatcgacgt cttcctcgtc
     5401 cgtctctatg tcctgttcat gaaccgcctc tacctggatc agctgtatct caagttcggc
     5461 cggatcgtga cgctcgtcgc ccatcacctg gaaaagcggc tgtcgtgacc atgcaacgca
     5521 gccacacgaa caacggcgcg gcccaggctg tcggaggcct gccatgatca agacctggat
     5581 gacaccgtgg ctgctcatgg ccgttccctt cctcggcgcg acgctcagcc tgctcctccg
     5641 cacgtcgctc cagcgaatga agacctcagc cctcctgacg acggtcgcta gtatgctcgt
     5701 cgtcatcggg acgccctggg cgctgggcga cacgctggcc ggtgtacctt ttctctgcct
     5761 gctcccgctg acggcgtttc tttcgctcct cggccaaccg ttacaccgcg acaatacttc
     5821 ggcctggacg acgacgctgg tgctgttggg actgggactg ggcatcctca ctgctcaagc
     5881 gccggcgcac gacgtcatgc tcctgatact gcccggcttg ctctgcatcc tgctgtatcg
     5941 gtaccaatcc ggcgccacgc cggagacctg gcgggggctc tcggcctacg gactgagcat
     6001 gatgtcggct gcgctggccc tggtgctccc gacaccggcc tcgacggtgg ctctgttggt
     6061 cacctgtgcc acac
//
LOCUS       DAORRQ010000129         6073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_464_length_6073_cov_47.215852, whole genome shotgun sequence.
ACCESSION   DAORRQ010000129 DAORRQ010000000
VERSION     DAORRQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_464_length_6073_cov_47.215852"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..614)
                     /gene="smbP"
                     /locus_tag="PK224_20210"
     CDS             complement(258..614)
                     /gene="smbP"
                     /locus_tag="PK224_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HRB18130.1"
                     /translation="MQTAKWRGVVIAGVMMALVGAPMVAGVAFAAGNKHQAEAVEHAK
                     EAVAHGKQGHADALVKHAEAALKHAEGAVAETKNPHVTEAIKGLKDGIEHGKAGHADV
                     ATKAVENALPHLSEGM"
     gene            complement(695..1588)
                     /locus_tag="PK224_20215"
     CDS             complement(695..1588)
                     /locus_tag="PK224_20215"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRB18131.1"
                     /translation="MQHPLLIDCETLQKRLGQPGLVVLDVRGRAAYEFGGHIPGAVHS
                     TWHEYSDPDAVAKGLLDPDLKRIEKRVQALGINQESEVVIYSNPFDNWGDEGRMFWML
                     EYLGHKNLRVLDGGWVKWIHERRPFEHGAVRCAPGNFVVSPVESAIIMKNELKGIARK
                     PNPTTTIVDARSLEEYLGKEISGIPRPGHIPGAVHVAWSGFLNADATVKDLAAINAQL
                     DLKGLSPTHETICYCTGGVRSAWLYFILRLAGYQRLRNYPGSWWEWSRDFACPVEKDL
                     LALQKLLGLSESQVPTGMRPS"
     gene            complement(1617..2891)
                     /locus_tag="PK224_20220"
     CDS             complement(1617..2891)
                     /locus_tag="PK224_20220"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRB18132.1"
                     /translation="MNLWVKILLSVAVLAGAIYLYYTEVKPVVIFGLRSDYAHAIPFQ
                     KVPEGLTSLKAESCGQCHREIYDEWKTSIHAQAYEDPFFQAYWKKDKNIWVCLNCHTP
                     LENQQPTLVKDIPRGRVEKATQEPNPHFDADLRKESITCAACHVRDGVILGPFDDSAA
                     PHPTKFDPSFRNAQFCSRCHNVVSGPAQFYNVGPCGTYAEYEGKYFMQERGFICQSCH
                     MPEVDRPVAENGPIRRGRRHLWRGGHDPDMVKRAVAIQVKADPPAPKPGEQVTVTLSL
                     MNAGAGHKIPTGDPDRHFTVEFTVQDSNRQVLAEKSHTMGRWIMWQPAIVELYDNRLL
                     PLASRDYQFAYRMPEASKGLTLKTRVRYHILTDGQHEMLKTKYGLMADDPYAFTVYER
                     EVPLNETLAAALVDPLPEPPPMACASPTVSKG"
     gene            complement(2888..3832)
                     /locus_tag="PK224_20225"
     CDS             complement(2888..3832)
                     /locus_tag="PK224_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRB18133.1"
                     /translation="MGTTGLIYDPRYLEHDMGAGHPESPNRLRAIMQRLEQSGTLATL
                     TRIDPRTAEDEWVTLVHRSEYVAMLNRQAPTHGRISLDADTSMSPGSLGAAYLAAGGA
                     LAGVDAIMAGRVQHAFCAVRPPGHHAEADRAMGFCLFNNVAIAARYAQKRYGVQRVLI
                     VDWDVHHGNGTQHSFESDPSVLFFSTHQYPHYPGTGRASECGRGAGEGLTINVPMEAG
                     EGDDEYRAVFQKVLVPAADAFKPEFVIISAGFDAHRDDPLASMGLTEEGYADLTNIVA
                     GIARQHCQGRLLSALEGGYNLTALAASVERHIQALVAA"
     gene            3978..4295
                     /locus_tag="PK224_20230"
     CDS             3978..4295
                     /locus_tag="PK224_20230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB18134.1"
                     /translation="MPNPRIEPLKRVLALEPDDDVAWFGLGKAYMEDANFEEAAKALR
                     QCITVKPTYSAAYYALAQSLQKLNRVEECRQVSDQGIEVSTKNGDAMVTKNLEALKSA
                     LPA"
     gene            complement(4433..4762)
                     /locus_tag="PK224_20235"
     CDS             complement(4433..4762)
                     /locus_tag="PK224_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18135.1"
                     /translation="MTEQEINRAIQYVTAATSYGRDTVAEVLRTGLSELAQLSTQSTC
                     RFEREDLMGYLCLWTMKRTGHPEPLVREVLDGAGRWLDEVATTVAQRREAQADGGDDD
                     EPGAATA"
     gene            4915..>6073
                     /locus_tag="PK224_20240"
     CDS             4915..>6073
                     /locus_tag="PK224_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRB18136.1"
                     /translation="MAVSRKIATATSSRRSSASREFADHWELSDLVTDPVKQFDRYLK
                     DLSAKVSRFESARAELSATMSEQAFLNLLTLSEQLARDSGRLGAYAYLWFSEDTKQLQ
                     ARSFKTKVEEHLTALQNHLLFFDLWWQSVDQRNAERLMANSGDFRYHLETIRRFKPHT
                     LSEPEEKIINIKNITGQSAVHQLYDVVTNGFTFTMRIGGKKKTMNREALSAYLRSPKA
                     AVREAAYREMYRVYESQQDLLGEIYKTLVNDWKAENLQLRHFKSPLESRNLSNDIPDR
                     AVDALLSVCMKNAPIFQRYFKLKAQLCKIKTMNRYHIYAPHRAEQKTYRYADAVRMVL
                     DAYYGFSPRLAELAQRVFADRHIDARTKPGKMGGAYCYSVTPNLTPYVMLNF"
BASE COUNT         1248 a         1937 c         1649 g         1239 t
ORIGIN      
        1 ggctccgcca gccggaacac ctcttcctgc gacacaaact gataacccga cgcgcacaat
       61 tcaggcaaca ccagaaggtc ggcctccgcc tcttcaagtc gagccgtgat gaggtcgaga
      121 ttgtgcgcga cctcgccgaa gaccggatca aattggagat acccgacccg catgatgtcg
      181 cctttctact caaagaaaaa cgggcaggga cggccctgcc cgtttcatct tacacgccgt
      241 cgacgcgcaa gaaccgatta cataccttcc gacagatggg gaagagcgtt ctcaacggct
      301 ttggtcgcca catcggcgtg gccggccttg ccgtgctcga tcccgtcctt gagccccttg
      361 atggcttcag tgacatgcgg attcttggtt tcggccaccg cgccctcggc atgcttgagc
      421 gccgcctcgg catgcttcac caaggcgtcc gcatggccct gctttccatg cgccacggct
      481 tctttggcat gctccaccgc ttcggcctga tgcttatttc ctgccgcgaa agccacaccg
      541 gcaaccatcg gcgccccgac gagcgccatc atcacaccgg ctatcacgac tccccgccat
      601 ttcgcagtct gcatgaacag cctcctctgt aagtatgtgc accgcatgat tccaacgata
      661 ctgtatttca gcataaagag gggcctgcat cctgtcaaga cggacgcatg ccggtcggaa
      721 cctgcgactc cgacaggccg agaagtttct gcaacgcgag cagatccttc tccaccgggc
      781 aggcgaaatc gcgactccac tcccaccagg agcccggata attacgcaac cgctgataac
      841 cggccagtcg caatatgaaa tagagccacg ccgagcgcac gccgccggtg cagtagcaga
      901 tcgtttcatg tgtcgggctc agccccttga gatccaactg cgcgttgatg gcggccagat
      961 ccttgaccgt cgcatcggca ttcaagaatc cgctccacgc cacatggacg gcaccgggaa
     1021 tatggccggg cctcggaatg ccggagattt ctttccccag atactcttcc aggctccgcg
     1081 catccacgat cgtggtcgtc gggttcggct tccgcgcaat ccccttcaac tcgttcttca
     1141 tgatgatcgc cgactctaca ggggacacga cgaagttgcc gggcgcgcag cggaccgcgc
     1201 catgctcgaa cggccggcgt tcatggatcc atttcaccca cccgccgtcc agcactctga
     1261 ggtttttgtg gcctaagtat tccagcatcc agaacatgcg tccttcgtcg ccccaattat
     1321 cgaaggggtt cgaatagatc accacttcgc tctcctgatt gatgccgagc gcctgcacac
     1381 gtttctcgat gcgtttcagg tcgggatcca gcaaaccttt agcgacggca tcggggtcgc
     1441 tgtattcatg ccaggtggaa tgcacggcac cggggatatg gcctccgaac tcataggcgg
     1501 ctcttcctcg cacatcgagc acgacaaggc ccggttgccc caagcgtttc tgaagcgttt
     1561 cacaatcgat gagtaatgga tgttgcatgg gttcctcgat ctcggtgggt gatgacttac
     1621 cctttggata cggtcggact cgcacaggcc atcggcggcg gctccggcaa gggatcgacc
     1681 aacgcggccg ccagcgtttc atttagcggc acttcccgct catagaccgt gaaggcgtaa
     1741 ggatcgtccg ccatcaggcc gtactttgtc ttcaacatct catgctgccc atcggtcagg
     1801 atgtggtacc gaacccgggt cttcaacgtc aaccccttgg acgcctccgg catccggtag
     1861 gcaaattgat agtcccggct ggccaagggg agcaagcggt tatcgtacaa ctctacgatg
     1921 gccggttgcc acataatcca gcggcccatc gtgtgcgatt tctcggccag cacctgccgg
     1981 tttgagtctt gaaccgtgaa ttctaccgta aagtgccggt ccggatcgcc ggtcggaatt
     2041 ttgtgccccg ccccggcatt catcagggag agggtcacag tgacctgctc acccggtttg
     2101 ggagccggag gatcggcctt cacctgaatc gccacggccc gcttcaccat gtcgggatcg
     2161 tgccccccgc gccagagatg tcgtcgcccg cgcctgatcg gcccgttttc ggccaccggc
     2221 cggtcgacct ccggcatatg gcaactttgg cagatgaagc cccgctcctg catgaaatac
     2281 ttaccttcat attcggcgta ggtcccgcag gggccgacgt tgtaaaactg cgccggaccg
     2341 gagaccacgt tatggcaacg cgaacagaac tgcgcgttgc ggaaactggg gtcgaatttc
     2401 gtgggatgcg gcgcagcgga atcgtcgaag ggtccgagga tcactccgtc gcgcacatgg
     2461 caggcggcac aggtgatcga ttccttccgc aggtcggcat caaaatgagg attcggttcc
     2521 tgcgtcgctt tctcgacccg cccccgggga atgtctttca ccagcgtcgg ctgttggttc
     2581 tccaacggcg tgtggcaatt caaacagacc cagatgttct tgtccttttt ccaataggct
     2641 tgaaagaacg gatcttcgta ggcctgggca tggatactgg ttttccactc gtcataaatt
     2701 tcccggtggc attgaccgca ggactcggcc ttcaggctgg tcaggccctc cggcaccttt
     2761 tgaaacggaa tggcatgggc ataatccgac cggagtccga aaatgacgac cggtttcacc
     2821 tcggtgtaat agaggtaaat cgccccggcg aggactgcga cactcagcag gattttcacc
     2881 cacaggttca tgccgccacc agcgcctgaa tatgtcgctc gaccgacgcg gccaacgccg
     2941 tgaggttgta cccgccttcg agtgccgaca gcaggcgtcc ttggcaatgt tgcctggcaa
     3001 ttccggctac aatattcgtc agatcggcat acccttcttc agtcaacccc atgctcgcga
     3061 gcggatcgtc ccggtgggcg tcaaaaccgg cggagatgat gacaaactcc ggcttgaagg
     3121 catcggcagc cggcaccaga accttctgaa acaccgcccg gtattcgtcg tcgccttccc
     3181 ccgcctccat cggcacattg atcgtgagcc cttccccggc tccgcggccg cactcgctgg
     3241 cgcgcccggt cccaggatag tgcggatact ggtgcgtact gaaaaacaac accgacggat
     3301 ccgattcgaa actgtgctgc gtcccgttcc cgtggtgaac gtcccaatcg acgatcagga
     3361 cccgttgcac gccataccgt ttctgcgcat accgcgccgc gatcgccaca ttgttgaaca
     3421 ggcagaaacc catcgcccga tcggcttccg catggtgccc cggcggacgg accgcacaga
     3481 aagcatgctg gacccggccg gccataatcg catccactcc cgccaaggct ccaccggccg
     3541 cgagataggc ggcgccgagc gatccgggtg acatggaggt atcggcgtcg agcgagatcc
     3601 ggccatgcgt cggcgcctgt cgattgagca tcgcgacata ctccgaccga tgcaccagcg
     3661 tgacccactc gtcctcggct gttcgcgggt caatcctggt gagtgtggcg agcgtaccgc
     3721 tctgttcgag ccgctgcata atcgcgcgca gccggttggg cgactccgga tgtccggcgc
     3781 ccatgtcgtg ttccaggtaa cgtggatcgt aaatgagccc ggttgttccc atgtcaattt
     3841 ccgtgaagcg tcgttcgtat ctcgtatttc gacacaaaca gtttcctcac cctatcatgc
     3901 aggaaagagc gtagacaatg agcggaaacg cgtgctacgg tttcgcttca ccgtgccgat
     3961 cgaccctgga gaagcccatg ccgaatccga gaatcgaacc gctgaaacga gtgctggccc
     4021 tcgaacccga tgatgatgtc gcctggttcg gactggggaa agcctatatg gaagatgcca
     4081 atttcgaaga ggccgccaag gccttgagac agtgcatcac ggtcaagccc acctactccg
     4141 ccgcttatta tgcgctggcg caatcgctcc agaagctgaa tcgggttgaa gaatgccggc
     4201 aagtgtcgga tcagggcatc gaggtgtcta ccaagaacgg cgatgcaatg gtgacgaaaa
     4261 atctggaagc gctgaagagc gcactccccg cctgaccgac cactccccaa tctcactcag
     4321 tcgcttggag ttggcggagt ccgtgaagcg tctctcgtga cacgtatttc gtaagagatg
     4381 acagatccac cgagggacct gtcacgaacg acaggcgtca tccacacgcc acttacgccg
     4441 ttgctgcgcc tggctcatcg tcatccccgc catccgcctg cgcctcgcgc cgctgggcca
     4501 ccgtcgtggc cacttcatcc agccagcggc ccgcgccatc gagcacttca cgcaccagcg
     4561 gctccggatg accggtgcgt ttcatcgtcc agagacagag atagcccatg aggtcttccc
     4621 gctcaaaacg gcaggttgac tgcgtggaca attgcgccag ttccgacaaa ccggtccgga
     4681 gtacctccgc caccgtgtcc cgtccgtacg atgtggcggc tgtcacatat tgaatcgcgc
     4741 ggttgatttc ctgttcggtc atgtcttcac cttgcggcgg attgtaacat tcgagccgca
     4801 cctgtcaacg gggcctctgc ctccggagac gcgtgtcttc gaagaatctc cacgcccttg
     4861 acctccgacg atggtatgat tccttctatc atttactttc taggagcctc gaccatggcc
     4921 gtttcgcgca agattgccac tgctacgtca tcacgtcgtt cgtccgcctc ccgtgagttt
     4981 gccgatcatt gggaactctc cgaccttgtc accgacccgg tgaaacaatt cgaccgttat
     5041 ctgaaagacc tgagcgcgaa agtctcccgc ttcgaatcgg cgcgcgcaga gctgtctgcc
     5101 actatgtccg aacaagcgtt cctgaatctc ctcaccctct cggaacagct tgcgagggat
     5161 tcgggtcggc tcggagccta cgcgtacttg tggttctccg aagacacaaa acagctccag
     5221 gcccggtcct tcaaaaccaa agtggaagaa catctcaccg ctctccaaaa ccatctgctg
     5281 ttcttcgatt tgtggtggca gagtgtggat cagcggaatg cagagcgcct catggcgaac
     5341 agcggcgact tccggtacca cctggagacc atccgccgct tcaagccgca tacgctctcg
     5401 gaaccggaag agaagatcat caacatcaag aacatcaccg ggcaaagcgc cgtccaccag
     5461 ctctacgatg tcgtcacgaa cggcttcacc ttcacgatgc gcatcggcgg gaaaaagaaa
     5521 acgatgaatc gcgaagccct ttcggcctac ctccgtagcc cgaaagccgc cgtccgagaa
     5581 gccgcctatc gggagatgta tcgcgtctat gagtctcagc aggatctcct gggggaaatc
     5641 tataaaaccc tggtgaacga ctggaaagcg gaaaatcttc agctgcggca cttcaagtcg
     5701 ccgctggagt cgcgcaatct cagcaacgac attccggatc gtgccgtgga tgcgctcctc
     5761 tccgtgtgca tgaagaatgc gccgatcttc caacggtatt tcaaattgaa ggcgcaactt
     5821 tgcaagatca aaaccatgaa ccgctaccac atctatgcgc cacaccgggc cgaacaaaag
     5881 acctaccgct acgcggatgc ggtccggatg gtgctggacg cctactacgg cttttcgccg
     5941 cgcctcgccg aattggcgca acgcgtcttc gccgatcgcc acatcgatgc ccgcacgaag
     6001 ccggggaaaa tgggcggcgc ctattgttac agtgtgaccc ccaacctgac accctacgtc
     6061 atgttgaatt tca
//
LOCUS       DAORRQ010000130         6014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_468_length_6014_cov_116.782010, whole genome shotgun sequence.
ACCESSION   DAORRQ010000130 DAORRQ010000000
VERSION     DAORRQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_468_length_6014_cov_116.782010"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /gene="groL"
                     /locus_tag="PK224_20245"
     CDS             complement(<1..1632)
                     /gene="groL"
                     /locus_tag="PK224_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRB18137.1"
                     /translation="MMAKQLLYSEAARAAILRGVNQLADAVKATLGPKGRNAILDKKF
                     GAPTITKDGVTVAKEVELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIYRE
                     GVKNITAGANPMEIQRGINKAVEVVIGELKKLSKPCQNKTEISQVGTISANNDKTIGD
                     LIAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMEAVMDEP
                     LILINEKKVSSMKDLLPVLEQVAKLGKPLIIIAEEVEGEALATLVVNKLRGTLNVSAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEDLGLKLENVKLTDLGRAKRVTIDKDNTTIVE
                     GHGDPKKIDGRVKQIKAQIEETTSDYDREKLQERLAKIVGGVAVINVGAATETEMKEK
                     KARVEDALHATKAAVEEGIVPGGGTAYLRCLKGLDSLKDLPLEQKVGVDIVKRALEEP
                     VRQIAANAGAEGSVVVGRVREDKNPNGGYNAATDEYVDMIKLGIIDPTKVSRCALQNA
                     ASVAGLMLTTEVMITELPEEKKEAAGGHAGHNHGME"
     gene            complement(1689..1991)
                     /locus_tag="PK224_20250"
     CDS             complement(1689..1991)
                     /locus_tag="PK224_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRB18138.1"
                     /translation="MATEAKDKKSTAAKNFQPLGDRLFVTYTEEMERTSGGIYVPDSA
                     KEKPQRGIVQAIGKKVENIKVGDQVLFDKYSGSKLRIEDEECLILKEEDILGIFTN"
     gene            complement(2303..3862)
                     /locus_tag="PK224_20255"
     CDS             complement(2303..3862)
                     /locus_tag="PK224_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18139.1"
                     /translation="MTAFGCRTATFFRIAIAGFLLAAVGETVSPVRSLADSPITRENT
                     RPGGTDWILTDPADHEVEGYASATSIQRGDKLHFYIHSTDPRITVAIYRMGWYAGAGA
                     RLVQGGISLPGVRQPMPSADPVTGLIECDWQVSYTLNTATDDPGEWLSGVYLAKLTGT
                     SSGKQSYIIFVVREDDRPADFLFQSSVTTFQAYNNWGGKSTYPSNSRDEQWARKVSFN
                     RPYAASQHPRGASGTGAGEFLTAMSIHPTRSLSTAGWEYNMVRWLERNGYDVTYSTDI
                     DTHSRPDFWKGHKAWLSVGHDEYWSDEMRRHVEAARDQGLGLGFLSANTCYWQIRLEP
                     SPLTTEPNRTMVSYKEVALTEDPYALDGDPSNDHLITVQWRDQPLNRPEHKLLGVMFE
                     TVPVDGDLVITRPSHPLFSGIPLPPDHRLPGLLGYEIDRAFPDGPAKLEILAHSPHQR
                     DGQVVYGDMTLYQAPSGAHVFATGTIQWSWGLDDYNAPALRTARTSEAAQQITRNILE
                     LLARKTLSAAQ"
     gene            complement(3843..4865)
                     /locus_tag="PK224_20260"
     CDS             complement(3843..4865)
                     /locus_tag="PK224_20260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HRB18140.1"
                     /translation="MTQRKHTHNSNSHADLDEDTDAIEPEALEPTDEEVTEEHDAADH
                     QPSKEPQEAPLSTSLVPVTTLQQYLTEIRRYPYLSKEEELRLFEEYQLRGSRDAAMKL
                     ILANLRVSVSIASEYLHTGADHMDLIQEGNVGLLQAIKKFDPTKNVRFYAYAAWWARA
                     YILRYLLNTYRLIKVGTTQDQRKLFYNLKKEKAKLEREGFAPDSKLLADRLNVRERDV
                     IEMDQRLGSWELSLDQPIGQENDGTLMDILPAHEQPADERLADTQLKALFRRKLAEFT
                     KTLDEREEDILRNRILSETPLTLEDMGAKYGITKERTRQLEARIIKRLRDYIKKDVKD
                     FDRLRM"
     gene            5081..>6014
                     /locus_tag="PK224_20265"
     CDS             5081..>6014
                     /locus_tag="PK224_20265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRB18141.1"
                     /translation="MSLSTLGVALRSGHWPTLAGAWLHLTVSFMVWLLFGALAVAIGQ
                     ELGLTATQQSVLVALPLLGGAALRITAGWACDWYGGKRTGLLVLGLQLLAVIWAALGG
                     RSYVELLGIALLLGAGGASFAVAMPVASRAYPATHQGLVLGLVASGNIGTVLVLLLAP
                     RWETALGWHWACGIMAVPILIAIGLFTTVVQSEPTVRTAGEGAWWQAAWAMARVPSVY
                     WLCMIYGVTFGGFVGLTSFLPVLLHDQYGSDPIVGGSIAALCGFTGSLIRPVGGYVAD
                     RWGGLPVLVAVFVALASVTVLAGSLPALPWAVGLL"
BASE COUNT          978 a         1694 c         1918 g         1424 t
ORIGIN      
        1 ttccatgccg tggttgtgac cagcatggcc accggcagct tcttttttct cttccggcaa
       61 ctccgtgatc atgacttcgg tcgtgagcat caagcccgcg acgctggcgg cgttctgcaa
      121 ggcacaacgc gacacctttg tcggatcgat gatgcccaac ttgatcatgt ccacgtattc
      181 gtcggtcgcg gcattatatc caccgttcgg attcttgtct tcgcgaaccc gaccgaccac
      241 cacggaacct tcggctcccg cgttggctgc gatctggcgg accggctctt cgagcgcccg
      301 cttgacgatg tccacaccga ctttctgctc caagggcaga tccttgaggg aatccaatcc
      361 cttgagacag cgcaggtagg ccgtgcctcc gccaggaacg atgccttcct cgacggctgc
      421 cttggtggcg tgcaacgcgt cttccacgcg cgccttcttt tctttcattt cggtctcggt
      481 ggctgcaccg acgttgatga ccgccacacc gccgacgatc ttcgccagcc gctcctgcag
      541 cttctcgcga tcgtagtccg aggtggtctc ttcgatctgg gccttgatct gcttcacgcg
      601 gccgtcgatc ttcttgggat cgccatgacc ttcgacgatc gtggtgttgt ccttgtcgat
      661 cgtgacgcgt ttggcgcggc cgagatccgt cagcttgacg ttctcgagct tcaagccgag
      721 gtcttcagag ataacctggc cgccggtcag gatcgcgatg tcctccagca tggccttgcg
      781 gcgatcgccg aagcccgggg ccttcaccgc cgacacattg agggtgccgc ggagcttgtt
      841 gaccaccaag gtggcgagcg cttcgccttc gacttcttca gcaatgatga tgagcggctt
      901 gccgagcttg gcaacctgct cgaggaccgg gaggaggtcc ttcatgctgc tgactttctt
      961 ttcgttgatc aggatgagcg gctcgtccat gacggcttcc atccgctcgg cgttggtcac
     1021 gaagtagggg gagatgtacc cgcgatcgaa ctgcatgccc tcgacgacgt ccagcgacgt
     1081 ggtcatcgat ttggcttctt cgaccgtgat cacgccatcc ttgccgacct tttccatcgc
     1141 ttccgcgatg aggtcgccga tggtcttgtc gttgttggcg gaaatggtac cgacttggga
     1201 gatttcggtc ttgttctggc aaggcttgct gagcttcttc agctcgccga tcacgacttc
     1261 cacggccttg ttgatgccgc gctggatttc catcgggttg gcaccagcgg tgatgttctt
     1321 gacgccttcc cggtagatgg cctgggccaa cacggtggcg gtggtggttc cgtcgccggc
     1381 cgtatcgctc gtcttgctcg cgacttcgcg aaccagctgg gctcccatgt tctcgtacgg
     1441 gttcttcagt tcgacttcct tcgccacggt cacgccgtcc ttggtgatcg tcggggcgcc
     1501 gaacttctta tccaaaatcg cgttccggcc cttgggaccg agcgtcgcct tgacggcatc
     1561 ggcgagctgg ttcaccccgc gcaggatggc cgcccgtgcc gcttcgctat acagtagttg
     1621 ctttgccata atcgtcctcc gttaatcgtc tagtagtcgt ttttcaggtg tttgggggtc
     1681 agacagaatt agttggtgaa gatgccgagg atgtcttctt ccttgaggat gaggcactct
     1741 tcgtcttcga tccgaagctt gctgccggaa tacttgtcga acaacacctg gtcgccgacc
     1801 ttgatgttct cgaccttctt cccgatggcc tgcacgatac cccgctgcgg cttttccttc
     1861 gcagagtcag ggacatagat cccgccggag gtccgttcca tttcctcagt gtaggtgacg
     1921 aacaaacggt caccgagcgg ctgaaaattc ttcgcagccg tggacttctt gtctttcgct
     1981 tccgtagcca taccgaatcc tccttctggt gatgagcgtc gaggccctaa tgtacagttg
     2041 tgtgaaagct ggtaaatcag ctttgacgtg ttaccgcaag ggctacctgc agtaaacccg
     2101 agacatagat agcgctagca agaggcaagt caatggatgg actataaaat tattctacgg
     2161 tgagatgcaa tgggataatg gcccctaagc tattgattct ctagagcatg ctttctgagt
     2221 cttcccacta tattttggag aagctcgccg caaggttgcg ccaagggctc aagtcggagt
     2281 cagaaggaag gcgaaatccc gattattggg cagccgagag ggtcttccga gccagcagct
     2341 ccaggatatt gcgggtgatc tgttgggcgg cctcactggt acgggcagtt cgcagagccg
     2401 gcgcattgta atcgtccagg ccccaactcc actggatggt tcctgtcgca aacacgtggg
     2461 ccccgctcgg ggcttggtag agggtcatat cgccatacac gacctgtcca tcccgctggt
     2521 ggggagaatg ggcaaggatc tccagcttcg ccggcccgtc ggggaatgcc cggtcgatct
     2581 cgtatcccaa caggccgggc aggcgatggt ccgggggcaa agggatgcca ctgaacagcg
     2641 gatgagaggg gcgggtgatg acgagatctc cgtccaccgg gacggtctcg aacatcacgc
     2701 cgagtagctt gtgttcgggt cggttcagcg gctgatcgcg ccattgaacg gtgatcaggt
     2761 ggtcgttaga cggatcgcca tccaaggcat agggatcctc cgtcagagcc acctccttgt
     2821 aggacaccat ggtccgattg ggttcagtcg tgagggggct gggttcgagc cggatttgcc
     2881 agtaacaggt attggcggaa aggaagccga gtcccaggcc ctggtcgcgg gccgcctcca
     2941 catggcgtcg catttcatcg gaccagtatt catcgtggcc gaccgacagc caggccttgt
     3001 ggcccttcca gaaatctggt cggctatggg tgtcgatatc ggtgctgtag gtgacgtcgt
     3061 agccattgcg ctccagccaa cggaccatgt tgtattccca cccggcggtc gagagggagc
     3121 gggtcgggtg gatcgacatg gcggtcagga actctcccgc tccggttccg ctggcgcccc
     3181 gcgggtgttg gctggcggca tagggacggt tgaacgagac tttccgcgcc cattgttcat
     3241 cgcggctgtt cgaggggtaa gtcgatttgc cgccccagtt gttgtaggcc tggaatgttg
     3301 tgacgctgga ctgaaacaga aagtcggccg gccggtcgtc ttccctgacg acgaagataa
     3361 tgtagctttg tttgccgctg ctcgtgccgg tcaacttggc gaggtagacg ccgctcagcc
     3421 attcacccgg gtcgtccgtt gccgtgttca gcgtgtacga cacctgccaa tcgcattcga
     3481 tcagtccggt caccgggtct gccgacggca tcggctgtcg cactcccggc agcgaaatgc
     3541 cgccttggac caaccgggct ccggcgcccg cataccaacc catccgatag atggcgacgg
     3601 tgatacgtgg gtcggtgctg tggatgtaga agtggagctt gtccccgcgc tggatgctgg
     3661 tggctgaggc atacccttcg acctcatggt cggctgggtc ggtgaggatc cagtcggtac
     3721 cgcccggtcg ggtattttcc cgggtaatgg gagagtcggc gagtgagcgg acgggtgaaa
     3781 cggtttcgcc gaccgccgcc agtaggaagc cggcgatggc gatcctgaag aaggtcgccg
     3841 tcctacatcc gaaggcggtc aaagtccttt acatccttct tgatgtagtc gcggaggcgt
     3901 ttgatgattc gcgcctccaa ctgccgggtg cgttccttgg tgatgccgta tttggcgccc
     3961 atgtcttcca gggtcaacgg tgtctcggag aggatgcggt tacgcaggat gtcttcttcc
     4021 cgttcatcga gggtcttcgt aaattcggcc agcttccgcc ggaagagcgc tttgagttgg
     4081 gtatcggcca gtcgttcgtc cgccggctgc tcatgcgccg gcaggatatc catgagcgtt
     4141 ccatcgtttt cctggccgat gggctggtcg agcgacagtt cccagctgcc cagccgttga
     4201 tccatttcga tgacatcgcg ttcgcgcacg ttgaggcgat ccgccaggag tttgctgtcc
     4261 ggcgcgaagc cttcccgttc gagcttggcc ttttcctttt tcagattgta gaagagcttg
     4321 cgctggtcct gggtcgtgcc gactttgatc aatcggtagg tattcagcag gtaccggaga
     4381 atgtaggccc ttgcccacca ggccgcgtag gcataaaagc ggacgttctt cgtggggtcg
     4441 aattttttga tcgcctggag caggccgacg ttgccttcct gaatcaaatc catgtggtcg
     4501 gcgccggtat ggaggtactc cgacgcaata gagacggaga cccgcaggtt ggccaggatg
     4561 agcttcatgg ccgcatcgcg actgcccctg agctgatatt cctcaaagag ccggagttct
     4621 tcttctttgc tcaggtaggg ataacgacgg atttctgtca ggtattgctg gagcgtggtg
     4681 accgggacca gggaggttga gagcggggcc tcctgcggtt ccttcgacgg ctggtggtcc
     4741 gctgcgtcat gttcctccgt gacctcttcg tccgtcggct caagggcctc gggttcgatg
     4801 gcgtcggtgt cttcgtccag gtctgcgtga gaattcgagt tgtgcgtgtg tttgcgctgt
     4861 gtcatagggg ttgaaactgt gggctaccag ccaggacgct catcatgaat tccgagcaag
     4921 ggctgactat accagatgtc gggtgccagg caacaacgtg ataggccggc tcgtccgcgg
     4981 ctcccttgcg tctctgaaat cgcgcctcgt atagtgcgtg gatgatgctg aaaaaccaca
     5041 cggatggcat ggacacagcc ggcgcggaat gaagtcgttg atgagcctca gcacgttggg
     5101 ggtagccttg cgcagcgggc attggccgac cctggccggc gcctggttgc atctcaccgt
     5161 gagtttcatg gtgtggctgt tgttcggggc cttggccgtg gcgatcggac aggaactcgg
     5221 gctgaccgcg acccagcaga gtgtacttgt ggcactgccg ttgctgggcg gcgcggcatt
     5281 gcgaattacc gccggctggg cttgtgactg gtatgggggg aagcggaccg gtctgctggt
     5341 gctgggtctt caactgctcg cggtcatctg ggcggcgctc ggagggcgga gttatgtgga
     5401 gctcctcggc attgcgctcc tgcttggtgc cggaggggcg agttttgcgg tggcgatgcc
     5461 ggtggccagc cgggcctatc ccgcgaccca tcaaggtctg gtgttgggcc tggtggcctc
     5521 gggaaatatc ggaaccgtgt tggtgctgct gctcgctccg cgatgggaaa cggcactcgg
     5581 atggcactgg gcgtgcggaa tcatggccgt ccccattctg atcgcgatcg gtctctttac
     5641 cacggtggtg cagagcgagc cgactgtgcg cacggcagga gagggggcct ggtggcaggc
     5701 cgcctgggcg atggcgcggg tgccgtcggt gtattggctt tgtatgattt acggcgtcac
     5761 gttcggcgga ttcgtcgggc tgacgagttt tctcccggta ttgttgcacg atcagtatgg
     5821 gagcgatccg atcgtcggtg gttccatcgc cgcgctctgc ggattcacgg ggagtctgat
     5881 tcgtccggtc ggcggatatg tggcggatcg ttggggcggg ctgccggtgc tggtcgccgt
     5941 gtttgttgca ctcgcttcgg tgacggtgct ggcggggtcg cttccggcgt taccctgggc
     6001 cgtaggattg ctgg
//
LOCUS       DAORRQ010000131         5962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_470_length_5962_cov_56.208735, whole genome shotgun sequence.
ACCESSION   DAORRQ010000131 DAORRQ010000000
VERSION     DAORRQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_470_length_5962_cov_56.208735"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1114
                     /gene="trxB"
                     /locus_tag="PK224_20270"
     CDS             200..1114
                     /gene="trxB"
                     /locus_tag="PK224_20270"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRB18142.1"
                     /translation="MHNVVIIGSGPAGLTAAIYAARANLSPLLIEGWQSGGQLTTTTE
                     VENYPGFSKGIMGPELMKEMRAQAERFGTIFLTGDVSAVDLAQRPFHLTIDGEQTVTA
                     ATLILATGASAIPLGVPNETRLIGHGVSTCATCDGYFFRGKDLVVVGGGDSALEEANF
                     LTKFARTVTVVHRRDKLRASKIMQDRAMKNEKISFAWNSVVEDILGTDLVTGARLKNL
                     VTGASSELACDGVFVAIGHRPNTDLFKSQLTMDEKGYILTSQGTTTSVAGVFAAGDVQ
                     DTKYRQAITAAGSGCMAAIDAERFLEHQ"
     gene            1267..2445
                     /gene="thiI"
                     /locus_tag="PK224_20275"
     CDS             1267..2445
                     /gene="thiI"
                     /locus_tag="PK224_20275"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HRB18143.1"
                     /translation="MRCAIVHYHELALKGRNRDFFEQRLVRNLRLALKDLKIRRIESL
                     QGRIRVTIPDEVTPDLVTERLRRTCGVSNFLLTESVPLDLTAPDLTTIKQVAERQLSE
                     QTFNSFRVTAKRADKRLPLTSMDVEREVGGYLHEATGKAVKLKQPDLTLYIELLTKEA
                     HVAARKIQGPGGMPVGTSGKVACLISGGIDSPVAAYRMMKRGCKAVFVHFSGRPLVSR
                     DSEEKVQELVQLLTAYQYHSQLYIVPFGEIQREIVANAPAAFRVVLYRRIMVRIAEEL
                     ARRHGCWALVTGDSLGQVASQTPENLSVVEAAAELPLLRPLIGMDKIEITEQAQQIGS
                     FTTSIEPDQDCCKLFVPLHPSTRTKPDDILRIERGLEISTFVKQGVEKAELSTFTFPS
                     "
     gene            complement(2429..3010)
                     /locus_tag="PK224_20280"
     CDS             complement(2429..3010)
                     /locus_tag="PK224_20280"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRB18144.1"
                     /translation="MRWVWLVSGCLLALVTVPVGSATAGEVGSTHLLRALVVQPDPDG
                     GVRATATLLFPGGPAVLQSILSEYRNWPELFETRMRVAQVEERDGRVLTDIYISHALL
                     PGEQRLLCESQALPGGGLITHLKGGDFTRYHREWRLQPAGDGTQTRAEFDLLVEVKTL
                     VPDWLVAVAMERELNTHFRLVRERALDRITKER"
     gene            complement(3304..4065)
                     /locus_tag="PK224_20285"
     CDS             complement(3304..4065)
                     /locus_tag="PK224_20285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB18145.1"
                     /translation="MAQAKILIVDDEVVVAEDIRRQLRALGYLVVGVVASGDEAVRLA
                     GEHRPDLVLMDIKLKGPMDGIDAARIIQTQYGAPVIYLTAFSDEETLERARETLPLAY
                     LIKPFVRSDLRAALELALFRQRVSRIAEQRGRWLDAVVQSMEDAVVTVDQQGRVTMLN
                     PAAEGLTGWSQPDALGKAVQEVMVVLDPESRRSVLHPAVQMLRKGTSADLGGRPFLLV
                     SRNGHEHRISDSAAMIRDERGDPSGVVLVFRPASA"
     gene            4385..4759
                     /locus_tag="PK224_20290"
     CDS             4385..4759
                     /locus_tag="PK224_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18146.1"
                     /translation="MSWLIRPILPLLLGCVLAWSLIPDEGQAQAPPDSSATTEQSCSF
                     GMTKGDPTHQCQVPIPAGCVIARFPGTDKPWTTISKAGRTFCTFDQKGTDWKTRITGQ
                     CTRCDSGHCTGQFMVRFECAKP"
     gene            4744..5790
                     /gene="queG"
                     /locus_tag="PK224_20295"
     CDS             4744..5790
                     /gene="queG"
                     /locus_tag="PK224_20295"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRB18147.1"
                     /translation="MREAVMSASLTIAIKQAARELGFDAVGISLLPTQTNPAVNLNRE
                     ASTGDLLDRLQEWLRRGYHATMAWMVRDPERRADPTQVLPGCRSIISVGMNYYTDYRA
                     DERQGNGRIARYAWGLDYHKILGDKLAQLAERIGALAPTSLNRAYVDTGPVMEKTWAQ
                     QAGLGWIGKHSNLVSTDFGSWLLLGEILTTLELEPDEAGTDLCGSCTLCIQACPTGAI
                     TEPYVVDAGRCISYLTIELRGTEPPIAEDLRRELGNRIFGCDDCLDICPYNVQAGPTT
                     EPGFQPSPLTGAPSLLALTKMDEQTFATTFKHSPIRRAKYAGLQRNLSWALSNETAPT
                     DRHSTTPPPSADPR"
BASE COUNT         1263 a         2010 c         1627 g         1062 t
ORIGIN      
        1 ttcgcggctg cgacaggcct gtcggcgggc gggctcgccg ctcggtcggt cgacatcctg
       61 ttcaaggatg cctcccttcc ttacggctcc acgcgcccgt ctggcttcgc gactccacac
      121 tttcgcgacg aaccgtcatg aataatgcgg gctagatcac agcttttccc tcgcggcttc
      181 ggtgtgttag catccgcgca tgcacaacgt ggtcattatc gggtccggtc cggccggcct
      241 caccgccgcc atctatgcag ccagagccaa tctctcgcca ctcctcatcg aaggctggca
      301 atccggcggg cagctgacga cgaccaccga agtcgaaaac tatcccggat tttccaaggg
      361 cattatgggc cctgagctca tgaaggagat gcgcgcccag gcagagcgct ttggcacgat
      421 attcctcacc ggcgatgtct cagccgtcga tctcgcacag cgaccgtttc acctgaccat
      481 cgacggggaa cagacggtga cggcggcgac cttgatcctg gccaccggcg cgtcggccat
      541 tccgctcggc gtaccgaatg aaaccagatt gatcggacac ggcgtctcga cctgcgcgac
      601 gtgcgacggc tacttttttc gcgggaaaga cctagtggtc gtgggcggcg gcgacagcgc
      661 cctggaagag gccaacttcc tgacgaaatt cgcccgaacc gtcaccgtag tccaccggag
      721 ggacaagctg cgggcctcga aaatcatgca ggaccgcgcc atgaagaatg aaaaaatttc
      781 cttcgcatgg aattccgtcg tcgaagatat ccttggaaca gacctggtca ccggggctcg
      841 actcaagaac ctcgtgacag gggcctcttc ggaactggct tgcgacggcg tcttcgtcgc
      901 catcggccat cggcccaata ccgatctctt caaaagccaa ctcacgatgg atgagaaggg
      961 ctacatcctc acctcgcaag gcacgaccac cagcgtcgcc ggcgtgtttg ccgcaggtga
     1021 tgtccaggat acgaagtacc gtcaggccat caccgctgcc ggctccggct gcatggccgc
     1081 cattgatgcc gaacgatttc tggagcacca atgaccgttg cgtctgatcg gccaggatgc
     1141 tcaaaatcgg catccaacaa ggccgcagct gagcgacaac cggaggcgta cccgcagtgg
     1201 tacgtcgagg attgtcgcga agcgagaacg aagttggtgg cgattttcag catcctgtaa
     1261 ccacttatgc gttgcgccat tgtccactac cacgaactcg ccctcaaagg ccgcaaccgc
     1321 gacttcttcg agcaacggct cgtccgcaat ctcagactgg cgctgaaaga tctgaagatc
     1381 cggcggatcg aatcgctgca ggggcgcatc cgggtgacga ttcccgatga ggtaacgccc
     1441 gacctggtga ctgaacgact ccgccgcacc tgcggagttt caaactttct gctgaccgaa
     1501 tccgtgccgt tggatctcac ggcgccggac ctcacgacca tcaagcaagt cgccgagcgg
     1561 caactctccg aacagacatt caactcgttc cgggtgacgg ccaaacgcgc ggataaacgc
     1621 ctgcctctga cctccatgga cgtcgaacgc gaagtcggcg gctatctcca cgaagccacg
     1681 ggaaaagccg tgaaactgaa acagcccgac ctgactctct acatcgaact cctcaccaag
     1741 gaagcccatg tcgcggcaag aaagatccaa ggcccgggcg gcatgccggt gggtaccagc
     1801 ggaaaagtgg cctgcttgat ttccggcggc atcgactccc ccgtggcggc ctaccggatg
     1861 atgaaacgcg gctgcaaagc cgtgttcgtg catttctccg gacgcccgct ggtaagtcgt
     1921 gattccgaag agaaggtcca ggaactggtc cagctgctga cggcctatca ataccattcg
     1981 cagctctaca ttgtgccctt cggcgagatt caacgtgaga tcgtcgcgaa cgccccggcg
     2041 gcgtttcgtg tcgtcttgta tcgacggatc atggtgcgca tcgccgaaga gctcgcgcgg
     2101 cgtcatggct gttgggcgct ggtcaccggc gatagcctcg gccaggtcgc atcgcaaaca
     2161 cctgagaatc tgtcggtggt ggaagcagcc gcggaactcc cgcttctgcg cccgttgatt
     2221 ggcatggaca agatcgaaat taccgagcag gcgcaacaga tcgggagctt cacaacgtcg
     2281 atcgagccgg atcaagactg ctgcaaactc ttcgtgccgt tacacccgag caccagaacc
     2341 aaacccgacg atatcctgcg catcgaacgg ggcctggaga tcagcacgtt cgtcaagcag
     2401 ggcgtcgaaa aggccgagct gtcgacgttc acctttcctt cgtaatccga tccagggctc
     2461 gctcccgaac cagtcgaaag tgggtattga gctcccgctc catggcgacg gcgaccagcc
     2521 aatcggggac aagcgtcttc acctcgacca acaagtcgaa ttccgcccgc gtttgcgttc
     2581 cgtcgccggc cggttgcagc ctccactccc gatggtaccg tgtgaaatct ccccccttga
     2641 ggtgcgtgat gagccccccg cccggcaacg cctgcgattc acagagcagc cgttgttcgc
     2701 cgggtaacaa cgcatgtgaa atatagatgt cggtgaggac ccgcccgtcc cgctcttcaa
     2761 cctgcgccac ccgcatccgg gtttcaaata gttcaggcca gttgcggtac tcggacagga
     2821 tcgactgaag caccgctggg ccgccgggaa acagcagggt ggccgtcgcc cgcacaccgc
     2881 cgtcaggatc gggctgtacc acaagcgccc gcagcagatg ggtagaacca acctctccag
     2941 ccgtcgcgct cccgaccggc acggtcacca gagcgagcag gcaaccggat accaaccaaa
     3001 cccatctcac gacgaatcgt agcctgtcca tcacagtata acggtggggt tcgtgaaccg
     3061 gacgaaattg cgcgcctcag acggacgtcg cacccggaaa attgcagaac cgacactcat
     3121 gcgttcctcc acaccttgaa accgtctcag tacccgggcg accggaatcg ctcgccggcc
     3181 ttcagcgctg ctcaagaagt atactcattg aatgcgccca gtgcgagcgc aacgacggga
     3241 cgaatacaat tatgcctgct gagcggatga tcacacggtt gaaaacggcg ggaacctgaa
     3301 cggttacgca gaggcagggc gaaacaccag tacgactcct gacgggtctc cccgctcatc
     3361 acggatcatg gccgcgctgt cgctgatccg atgctcgtgc ccgttgcgac tcacgagaag
     3421 aaacgggcgc ccgccaagat cggcagacgt tcccttacgc aacatctgta cggccggatg
     3481 cagcacggac ctccgactct ccggatccaa caccaccatt acttcctgaa ctgccttccc
     3541 cagggcatcg ggctgcgacc agccggtcaa tccctcagcc gccggattga gcatcgtcac
     3601 ccgtccctgc tggtcgaccg tcacaacggc atcttccatc gactggacga ccgcatccag
     3661 ccaccgcccg cgttgctcgg caatacgcga cacacgctgc cggaagagcg cgagttccag
     3721 cgccgcgcgc aaatcgctcc tgacaaacgg cttaatcaaa taggccaacg gcagggtttc
     3781 cctcgcccgc tccagtgtct cctcatccga aaaggccgtc aggtagatca ccggtgcgcc
     3841 atactgggtt tggatgatcc gggccgcgtc gatcccatcc atcggcccct tcagcttaat
     3901 atccatgaga actaaatccg gccgatgctc cccggccagc cgtacggcct catccccgga
     3961 tgccaccaca cccacgacca gatacccgag tgcgcgcagc tgacgtctga tgtcttcagc
     4021 cacgaccact tcgtcatcga cgatcagaat ttttgcctgc gccatagtac aacccgatga
     4081 aacgagatcg gaaaattggt atcgccgctc acaccacaca ccgctcgagc gcactacaca
     4141 cgtattcgca cagcgctcga taccccaccg caacgcaact aacatgccac ctggccttcc
     4201 ctgtactggc atcatgttat gccgggcggg attcttgccc ccatacacca acggtggttt
     4261 ttgaacagtc cttggacaga atcggcagaa aacaaggctt gcgtggacca ggcgctccgg
     4321 gaaagattcg ctgcggaaat gaccgctgtg atacgatgcc gctctttaac ggagttgatc
     4381 caacatgtct tggctgattc gtccgatcct ccccctactg ctcggctgtg tcctcgcatg
     4441 gagccttatt cccgacgagg ggcaggccca agcgccgcct gactcctccg cgacgaccga
     4501 acaatcctgc agtttcggga tgacgaaggg cgatcccact caccagtgcc aagtgccgat
     4561 tcccgccggc tgcgtcatcg cccgcttccc gggaaccgac aaaccgtgga cgaccatctc
     4621 caaagccggt cgcacgtttt gcaccttcga tcagaaaggc acggactgga agacgcgcat
     4681 taccggccaa tgcactcgct gcgattccgg ccattgcacc gggcaattca tggtgcgatt
     4741 cgagtgcgcg aagccgtaat gtccgcgtcc ctcacaatcg ccatcaaaca agccgcccgc
     4801 gaactcggct tcgacgccgt cggtatcagc ctccttccta cgcaaacgaa cccagccgta
     4861 aatctgaatc gcgaggcctc gacaggtgac ctgctggacc ggctccaaga atggctcagg
     4921 cgcggatacc acgcgaccat ggcctggatg gtccgtgatc ccgagcggcg agccgatccc
     4981 acccaagtac tgcccggctg ccgatcgatc atttctgtcg gcatgaacta ttacacagac
     5041 tatcgggccg acgaacgcca gggaaacggg cgcattgccc gctatgcctg gggcctcgac
     5101 taccacaaga tccttggtga caagctggcc caactcgccg agcgcatcgg cgcgctggcc
     5161 ccgaccagcc tcaaccgggc ctatgtcgac acggggccgg tcatggagaa gacctgggcc
     5221 cagcaggccg gactcggatg gatcggcaaa cactcgaacc tcgtctcgac agacttcggc
     5281 tcctggctgc tcctgggaga aattctgacg accctggagc tggagccgga tgaggcggga
     5341 accgatctct gcggaagctg cacgctctgc atccaggcct gtccgaccgg cgcgatcacc
     5401 gagccatacg tcgtcgatgc cggacgctgc atctcctacc tgaccatcga acttcgcggc
     5461 accgaaccgc cgatcgccga ggacctccgg cgagagctgg gcaaccggat tttcggctgc
     5521 gacgattgcc tggacatctg cccctacaac gtccaggccg gaccaaccac cgaacccggc
     5581 ttccaacctt ccccgctcac cggtgccccg tcgctgctgg ctctgacgaa gatggacgag
     5641 cagaccttcg ccaccacgtt caaacacagt cccatccgcc gcgcaaaata cgccggactc
     5701 caacgcaatc tctcctgggc gctctccaat gaaaccgccc ccaccgatcg gcacagcacc
     5761 acacctccgc cgagcgccga tccccgctaa gccccaccca ctgtttctcc ccggccgata
     5821 gtggtaggct acggtctcat gtcctccctc atgtgaggac ccacctcagc cggttgcgag
     5881 gccatgcacg cacccaccct cctgatcgtc gaagatgaag aacgaatgcg ccggctcttc
     5941 gagctggtgc tgaaacctgc cg
//
LOCUS       DAORRQ010000132         5861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_476_length_5861_cov_488.312263, whole genome shotgun sequence.
ACCESSION   DAORRQ010000132 DAORRQ010000000
VERSION     DAORRQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_476_length_5861_cov_488.312263"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PK224_20300"
     CDS             complement(<1..365)
                     /locus_tag="PK224_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18148.1"
                     /translation="MSQIISTVVIQHAEEAASLWTLRCYAGGAPQFRIFDIARLDERI
                     EAHVDGLRIACEPGWKLCKAALGNEESGEVFVASIIAYESGIESRIQTVLDAVEKNAE
                     ITKALVSGLGWIPFQQVAP"
     gene            complement(443..1213)
                     /locus_tag="PK224_20305"
     CDS             complement(443..1213)
                     /locus_tag="PK224_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18149.1"
                     /translation="MAEQRLTLPFQTEVRIPVIPEFQLTIRLCGKDSEGKLEIAQDIV
                     QQFVSASEAGMFSRPDVHPLQSSIKIVSSTGTTHGEVTYVCDTKGIDIGAFRILLSMI
                     TQCHYLHEPLRECELKSSAQAMTFYRLSEIVNQEVPRKFSKIPFHLSIEKNLSKSRRP
                     VVRIEFERKLEDVEFERVSNLIVVWETLVVLGGYLNTFDPMEELPLHPGELYMVTPRK
                     LEYTISEFSGPVHAFDGMVNMAVKLHASLSPLVSLEIE"
     gene            complement(1252..2205)
                     /locus_tag="PK224_20310"
     CDS             complement(1252..2205)
                     /locus_tag="PK224_20310"
                     /inference="COORDINATES: protein motif:HMM:NF023304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HRB18150.1"
                     /translation="MHPEDRVIKTMHRARESSLENIVPPAVRLRLKVCLDITVYWTGS
                     VFQHGAGVLDFYRQSMELLGKHIKHYETETMGGACPVDADSLHLLPFWIEGTKARRSI
                     YALFLESASEPNVPSTRAFSFRAWEAREVPVGMMRLVLPTDFFGKEPVAFVDLTKRLV
                     RKLLFESGHAGYALNHYERGPFEHDALEAMYVLSRKYPGLDIPSPTSTLFVISEGLKR
                     VNWLTLLGSSLSDRLGGVVKLKNEMGEEIDVYALDHGVLIQAGPEPEIGDVNRRGWLP
                     FYHHVGRVLAPIRAKNHPGFFMHGMDFDEEATEEWLARFDS"
     gene            complement(2737..3126)
                     /locus_tag="PK224_20315"
     CDS             complement(2737..3126)
                     /locus_tag="PK224_20315"
                     /inference="COORDINATES: protein motif:HMM:NF025047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HRB18151.1"
                     /translation="MGISVAVNPPKTPVTEGSNDIAAATLPNVCKMPAPPAPFVPTPL
                     PNIGKSSDHLTDCTTTVLIEGKKIAIKGSSYKSTTSPDMASKASGAGLISSTEEGKTE
                     FAAPGSMNVKAEGKNIQLLGDAMTNNG"
     gene            complement(3111..4076)
                     /locus_tag="PK224_20320"
     CDS             complement(3111..4076)
                     /locus_tag="PK224_20320"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRB18152.1"
                     /translation="MIDKVGEVMSVAIDLMLPPEMTNSDRMYQFACAAMEEALRPLIQ
                     FRIEGVSLPIIMGLPEERPGYSTAIGDKLIRQLTISENGPFKFTRVMALHEGHAAGLM
                     AIEKAAELIKGGQTTWCLAGGVDSYMNPDTLEWLDDGGQLMSSENRSGFPPGEGAGFL
                     LLASTAAAEKWKLPVLAWIIATSTAHEEKRIKTETICIGEGLTAAIQGVITKLNLPDE
                     KINTAYCDMNGERYRNEEFTFTLLRTQLAFVDAIDNITPADCWGDVGAASGPLFANLA
                     IASGLRGYARGPRPLLWASSECGKRSAALLHLAMHHQPGVQWASR"
     gene            complement(4258..5277)
                     /locus_tag="PK224_20325"
     CDS             complement(4258..5277)
                     /locus_tag="PK224_20325"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HRB18153.1"
                     /translation="MLRNRTPYAAERTWVLDKNAVKSWVVVVKGTFDICPDGSTKIAD
                     KQEDPLLACQYTGEPGKSSLLYEADLTPSKPATDVILNGHVYAPAGKPTRSTLATLTV
                     GSHSKSIEAFGDRRWKKGLLGGLSLTRPEPFEKLPLVYERAFGGWDSVDKDKSKHRLY
                     SKNPIGSGFWVTKEHAVDKPLPNFEDPRELISSWKDRPRAMSFGALASYWTPRLEYSG
                     TYDDRWQKTRFPLLPENFDGRHYQAAPEDQQVVDYLCGGEAVKLENLSPTGVLTFTLP
                     KIWFTFTTRFGRERVEHRAKLSSVIIEPEIPRLMMVWHSSLECHHKVDQLDETIIGQK
                     EYVSL"
     gene            complement(5331..>5861)
                     /locus_tag="PK224_20330"
     CDS             complement(5331..>5861)
                     /locus_tag="PK224_20330"
                     /inference="COORDINATES: protein motif:HMM:NF040117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HRB18154.1"
                     /translation="QRTMLKRTGRDDLPGFSVDAIESKETLQKISGAVVGMLMGQRAG
                     QYLVEYRENQTGDFVVARSVVEITEKDVGREVVLLFEQGCPDKPIIMGVIQPQTRSTG
                     GVLLDQILEKADELPSVTVDEERIELSANREVVLRCGKASITLTRVGKVLIRGEYVLT
                     RATGVNRVKGGSVQIN"
BASE COUNT         1386 a         1654 c         1299 g         1522 t
ORIGIN      
        1 tgtggtgcaa cttgctgaaa gggaatccac cctagccccg aaaccaaagc cttggtgatc
       61 tccgcgtttt tctcgacggc atcgagcacg gtttggatgc gtgactcaat cccagactcg
      121 tacgctatga tcgaggcgac gaacacttcg ccagattctt cgttcccaag ggcggctttg
      181 cacaatttcc acccaggctc acaagcaatc cgcagaccgt ccacatgagc ttcgatacgt
      241 tcatcaagcc tcgcaatatc gaagatccga aattgaggag cccctccagc atagcagcgc
      301 aacgtccaaa gtgaagccgc ttcttcggca tgctgaatta ccacagtgga tataatttga
      361 ctcatctcat catgtgagcg caatttactt cacccgcata gcacatactg tggtcgccct
      421 caacaattat tccctagtga tgctattcaa tttccagact tactaaaggt gagagagatg
      481 cgtgaagctt cacagccatg ttgaccattc cgtcaaaagc gtgcaccgga ccactgaact
      541 cagagatggt gtactcaagt tttcgcggcg tgaccatata cagttcacct ggatgtaacg
      601 gcaattcctc catcgggtca aacgtattaa gatacccccc aaggaccaca agcgtttccc
      661 acactacgat caggttactc accctctcaa attcgacgtc ctcaagcttt ctctcgaact
      721 ctatccgaac gaccggcctc cgactcttgg ataaattctt ttcaatactg agatggaatg
      781 gaattttgga gaatttccta ggcacctctt ggtttacaat ttcactgaga cgataaaatg
      841 tcatagcctg agcagaggac ttaagctcac attcacgcaa tggttcatgc agataatgac
      901 attgcgttat catgctcaag agaattctaa aagcaccgat atcgattccc ttcgtatcac
      961 agacatacgt aacctcacca tgcgtcgtac ccgtagaaga tacgattttt atcgatgatt
     1021 gtagtgggtg gacatcaggc ctggagaaca tgccggcctc gctagcggaa acgaattgct
     1081 gaactatatc ttgcgcaatt tccagctttc cttcggagtc cttgccacaa agacgaatag
     1141 tcagctgaaa ttctggaatt acaggaatac gaacttcggt ttggaatggc aatgtcagtc
     1201 tttgctcggc catcttaata ctcctttagc ctagaacatc gaacaggttg gctatgaatc
     1261 aaacctagcg agccattcct cagtagcttc ttcgtcaaaa tccatcccat gcatgaaaaa
     1321 tcccggatgg tttttcgcgc ggatcggagc cagcactctg cccacatggt gatagaaagg
     1381 aagccaaccg cgccgattca catcgccaat ctctggttcg ggtccagctt gaattagaac
     1441 cccatggtct agagcgtaga catctatttc ctcacccatc tcattcttta gctttacgac
     1501 cccacccaat cgatcagata aagaggagcc aagcaacgtt agccaattga ctctcttaag
     1561 tccttcacta ataacaaata acgtgctggt ggggcttgga atgtcgagcc caggatattt
     1621 tcgacttaat acgtacattg cctctaatgc atcgtgctca aagggacctc tctcataatg
     1681 gtttagggca tacccagcgt ggccagattc aaacaacaac tttctgacca accgttttgt
     1741 taagtccacg aatgcaactg gttccttgcc aaaaaaatct gtcgggagta ccagcctcat
     1801 catccctaca ggcacctccc tagcctccca agcacgaaag ctgaaggccc ttgtggatgg
     1861 cacattcggt tctgacgcac tttcaagaaa caaggcataa atagagcgtc tagcttttgt
     1921 gccctcaatc cagaagggaa gcagatgcag cgaatcagcg tcaactgggc acgccccgcc
     1981 catcgtttca gtctcgtagt gcttaatgtg ttttccgagc aactccatac tttgtcgata
     2041 gaaatctaaa acccctgcac catgctggaa cactgaccca gtccagtaga cagttatatc
     2101 taggcatact ttgagacgta gcctaactgc tggcggtacg atattctcaa gcgaactttc
     2161 cctggctcta tgcatggtct taattaccct atcttcgggg tgcattatca gtcatattcc
     2221 cgaggccctc cattgcttca tggggggcaa ttgacgcaag acgtacacac cccaccgata
     2281 ttcggacacg cgattaggat tggcctcttc ccttcgcact gctgtgcctg acgatccagt
     2341 tggtctgcac tcttcatccc ccttcctacc ttatcgccag gcaacttaat ctcgaaagtg
     2401 cgctgtccgg ggccaacaat tctgttaccc gaaagcggtg cgcccagaat tgcatcaggc
     2461 ttgatcatca cggctccata ctgcctacca aatctttgaa acatggctgt ggccgcccgg
     2521 ccatactttg cagccgtact agctagaaca ttaacgtatc cgaacgtcct gaactgcaca
     2581 ccccttgcaa tcatccttgg atccgccgcc aatcgatttt caaggtcgtt cgagctttca
     2641 ccattggcga tcgcattaca gagttcctcg cacattatat ttgcaatgtc ttgctcgtcc
     2701 tgaccgagct gatagttccc tggaatggtg gcagcattag ccattgttcg tcatcgcatc
     2761 gccgagtaac tgaatgttct tgccctcggc ctttacgttc atcgacccag gagcagcaaa
     2821 ctctgtcttg ccttcttcgg ttgaagaaat caggcctgcg ccgcttgcct tactggccat
     2881 gtcaggggaa gtcgtacttt tgtaggaaga gcccttgatg gcgatctttt tcccttcgat
     2941 cagtacagtc gtagtgcaat cggttaggtg gtcggacgat ttcccgatgt taggtaatgg
     3001 cgttggaacg aatggcgctg gcggggcggg catcttacag acatttggca atgtcgctgc
     3061 agcgatgtca ttactaccct ccgtgacggg agtcttgggt ggattgacgg ctaccgagat
     3121 gcccattgaa cacctggttg atgatgcatg gctaggtgaa ggagcgccgc gctccgctta
     3181 ccgcactcgg aactggccca caggagaggt cgtggccctc gcgcatatcc acgcaacccc
     3241 gaggcaatag cgaggttggc gaacagtgga cccgaagctg cacccacatc cccccaacag
     3301 tctgcggggg taatattgtc tatcgcgtcc acgaatgcga gctgcgttcg taataatgta
     3361 aaagtaaact cttcgtttcg atatcgttcg ccattcatgt cgcagtacgc ggtattaatc
     3421 ttctcatctg gtagatttaa ctttgtgatt acaccttgga tcgcagccgt caacccttct
     3481 ccgatgcaga tggtctcagt cttgatgcgt ttctcttcat gtgccgtcga agttgcaata
     3541 atccatgcca gtacgggtaa tttccacttt tctgcggcgg ctgtggaggc cagaagaaga
     3601 aatcctgcac cctctcctgg tggaaagccc gacctattct cgctagacat cagctgacct
     3661 ccgtcatcga gccactccag tgtgtccgga ttcatatacg aatcaactcc gcctgctaag
     3721 caccatgtgg tttgcccccc cttgattaac tctgccgctt tttcaatcgc catcaaccca
     3781 gccgcatgcc cctcgtgaag ggccatcaca cgagtgaatt tgaacgggcc gttctcagag
     3841 attgtcaatt gccttatgag tttgtccccg attgcggtgg aataccctgg gcgctcttcc
     3901 ggcaacccca tgatgatcgg cagtgacaca ccttcgattc gaaactgtat gagaggtcgc
     3961 agtgcttctt ccattgccgc acaggcgaac tggtacattc gatcactatt cgtcatttca
     4021 ggtggaagca ttaggtcgat ggcaacggac atgacttcgc ccaccttatc gatcatgaag
     4081 ggatgttccc gaatggcact gatggcaccc ctgactgctg ctgctgttga tggtccatta
     4141 agaccaagag gcgttcgagc gcccacccct accacacaga caccgtactt attattggga
     4201 gatgcgtgac tcattccgat tgcgcccttt aacctctttt atggttacac tctggcacta
     4261 gagggacacg tactcctttt gcccaatgat ggtctcatca agttgatcaa ctttatgatg
     4321 acattcgaga ctagaatgcc acaccatcat cagacgtgga atttctggct caatgatgac
     4381 gctcgacaac ttcgcccggt gctcaactct ttccctccca aatctcgtcg taaatgtgaa
     4441 ccagattttt ggcaaagtga acgtaagcac acccgttgga ctgaggttct caagcttaac
     4501 ggcttctcca ccacagagat agtcaaccac ttgctgatct tcgggcgctg cctggtaatg
     4561 acgtccgtca aaattctcag ggagcagtgg gaatcgcgtc ttctgccatc tgtcatcata
     4621 ggttccagag tattccagcc tcggtgtcca gtagcttgca agagctccga agctcattgc
     4681 ccgcggacga tccttccatg aagagatgag ctcacgagga tcctcaaaat taggcaaagg
     4741 cttatcaacc gcgtgttcct tggttaccca gaaccctgag ccaatgggat tcttagagta
     4801 cagcctatgt tttgacttat ctttgtctac gctatcccac ccaccgaatg cccgctcata
     4861 cacaagcggt agcttttcaa aaggttctgg cctcgtcagt gataacccac caagcaaccc
     4921 ttttttccaa cgacggtcac cgaaggcttc gatagatttg gagtgactac cgactgtgag
     4981 agtcgccaga gttgaacggg ttggcttccc agcaggtgca tacacgtgtc cgttcaggat
     5041 gacatctgtt gccggcttgg acggtgtgag gtcggcctcg tataggaggc tagactttcc
     5101 aggttcacca gtgtattggc aagctagcag cgggtcttct tgtttgtcgg ctatcttcgt
     5161 tgaaccatcc ggacagatat cgaaagtccc tttcacaacc acaacccaac tcttgaccgc
     5221 attcttgtcg agaacccatg ttctctccgc tgcatacggt gttctgtttc ttaacatcca
     5281 catactgatc gagcaggcac actacggttg aactttgctc tcttctccgt ctaattaatc
     5341 tggactgaac cacctttaac ccgattcact cccgttgccc gtgtcaggac gtattctcct
     5401 cggatcagta ccttcccgac cctcgtgagc gtgatactag cctttccgca tctcaggacc
     5461 acttctcgat ttgcagaaag ctcgatcctt tcctcgtcta ccgtaacaga tgggagttcg
     5521 tctgcctttt cgagaatctg gtctaacaga actcctccag tgctcctggt ttgtggctgt
     5581 atgaccccca tgatgatcgg cttatcaggg catccttgct caaacagcaa gacgacttct
     5641 cggcccacat ctttttcggt aatctccacg acagaacggg cgaccacgaa gtcccccgtc
     5701 tgattctccc tatactcaac cagatactgc ccagccctct gccccattaa catacccacg
     5761 acagccccac tgattttctg caaagtctct ttgctctcta tggcatccac agaaaaccca
     5821 ggtaagtcat ctctcccggt tctttttagc atcgttctct g
//
LOCUS       DAORRQ010000133         5540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_493_length_5540_cov_41.208204, whole genome shotgun sequence.
ACCESSION   DAORRQ010000133 DAORRQ010000000
VERSION     DAORRQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_493_length_5540_cov_41.208204"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..666
                     /locus_tag="PK224_20335"
     CDS             298..666
                     /locus_tag="PK224_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRB18155.1"
                     /translation="MENTKPPATGLRISDDPMYRLLREGCIKEFNVKRAGGEPVDLTR
                     CDLRGLDLRGLETDGLDFSECYFRQADLRGIDFRNAKLEGASINAAKISGAYFPTELS
                     ASEIELSLLHGTRMRYQPKP"
     gene            complement(682..3630)
                     /locus_tag="PK224_20340"
     CDS             complement(682..3630)
                     /locus_tag="PK224_20340"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HRB18156.1"
                     /translation="MTLLSRYRTALIVLCGIVALYALLGFVVAPYAVKTYAIPALAER
                     LRHPVVLGDVRINPFTFSLMLTAFEIREPDQTPMLGFEELFVDFEGTSLVRSAYLFDE
                     IRLTLPFGLVHIRADGTLNLLGLVPPAQDSPADPAPPADAKAEKAPVPPVEIRLLSIR
                     QGVIEYRDDSKRKPVTIDVVPIEITLRNFGTRRGGENAYAFSAEFDEGETLAWEGTVH
                     LDPLESDGHVSLSNVKLNTFWPSLRDRFRFDILSGAVLVDARYHFDTSAAPVNLQVNE
                     GKVLLSDFRLSAAGERDPVMTVPALAFEGIRMDLPKREAGVGTIALSGAEIRAWLAED
                     GVVNFKPLFTPVATSREDPVEPQRPATDSGQPWSVDVQAIEVTKAQVAFEDRSLKTPA
                     QLAIDDLHVTVNGIHVPLKGSWPVAAGFRLNQQGTVESKGTLQLDPLQTALTLKLAHI
                     GLRPFQPYLDRRMQVEIRDGELELDGELVYRSQHDPEPMIQYTGQLGLNSLHVADGVS
                     AHEFLGWTALGLNKVALEVAPTKVKIGEIAWRDPAIRFVTAKDGTTNLSRVMKAPAEG
                     PAQPPVSKPVEATVPKKATAPIPIDVNVVRLSKLSATFIDESIEPVITTGIQNLSGTI
                     KGLSSKQIAKAEVALTGTVDEVAPLKIQGQVNPLSEDTYTHLTFLFKGVDLTAVSPYA
                     GKYVGYPITKGKLSLDLMYQVSKQQLVGENKVLVDQLTFGEKTDSPDATSLPVRLAVG
                     LLKDRRGRIDIDMPVRGDLNEPDFRYGRVVLNALANLITKVATSPFSALGGLVGGSGE
                     DLQFIEFMAGSEVLESVEQRKIESIAKALQERPALRVDVAGAADPARDREALALQKIV
                     TEVQRRFTQGGTKNLQAVPSPSREFELLSDLYAEKLGKQPMKREELPGGKFVERVLTA
                     DELRQQLFPAMTVEESELRLLAQGRARAIREQLIEQGRLPEDRVFLIDAELTPSEGKQ
                     VRVRLNLTGS"
     gene            complement(3652..3728)
                     /locus_tag="PK224_20345"
     tRNA            complement(3652..3728)
                     /locus_tag="PK224_20345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3692..3694),aa:Arg,seq:tct)
     gene            4401..4709
                     /locus_tag="PK224_20350"
     CDS             4401..4709
                     /locus_tag="PK224_20350"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRB18157.1"
                     /translation="MATRALRTEKLDLRVSVSTKKTLEAAASVSSRTVSAFVLESALA
                     RADEALADRRTFHLSKAKWTEFLAALDAPTRPLPQMQRLLAEPGFFDATPAPGFKEKR
                     "
     gene            4706..5182
                     /locus_tag="PK224_20355"
     CDS             4706..5182
                     /locus_tag="PK224_20355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB18158.1"
                     /translation="MTRRIEKLQPHHAVEAFDCGHEVLNRFLQKHAVQNQFSGGSQTY
                     VGLVDNIVVGYYALAVGSVEQDNAPERVKKGLAKHSIPIMLLARLAVDLHWQKQGVGA
                     ALLKDATLRTLQAADIAGIRALVVHAKDNVAKKFYERFDFLPSPSDALHLFILLKD"
     gene            complement(5210..>5540)
                     /locus_tag="PK224_20360"
     CDS             complement(5210..>5540)
                     /locus_tag="PK224_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRB18159.1"
                     /translation="TCSDRPTKNMLQRPLESAQYAATSYQRRLTEYGLIPSMSRKGNC
                     WDNACVESFFGTLKRELVHQRPYVTRDEAKQDIFEYIEVFYNRQRQHSTLGYHAPVEF
                     EARAAVA"
BASE COUNT         1176 a         1685 c         1494 g         1185 t
ORIGIN      
        1 ccgcccagcc actcatgaaa gttgatcgtc ccttccgtcg tctcagctgt aaagtttggg
       61 gcatcgtcgc ccaatcgtag tcccatgatt tcctccttca atatccggtc tgacctggtg
      121 atggcactgc gtaagggagc agaaagacga gattagagta ccgtttctcg tcagtccggc
      181 gcaagcggaa gcttgcgcga agaatgccgc aagaccggtg gcgggacgaa ccgcctgccg
      241 cccctcaagt tttcgctcac gtatccgata agaaactcag gtgccacagg agagcgcatg
      301 gagaacacga aaccacccgc caccggactc cggatctccg acgatcctat gtaccgcttg
      361 cttcgagagg ggtgcatcaa ggagttcaac gtcaaacggg cgggaggcga accggtcgac
      421 ctgacccgct gcgacttgcg gggcctcgac ctgcgtgggc tggagacgga tggattggac
      481 ttcagcgagt gttactttcg gcaagccgat cttcgaggta tcgacttccg caacgcgaag
      541 ctcgaaggag ccagcatcaa tgcggccaag atttccggag cctactttcc caccgaattg
      601 agcgcgagtg aaatcgagct ctcactgctg cacgggacac gaatgcggta ccagcccaag
      661 ccctgaccct acccagaacg cttaacttcc cgtcagattc agcctgaccc ggacctgttt
      721 tccctctgaa ggcgtcagct ccgcgtcgat cagaaagacg cgatcctcgg gcaacctgcc
      781 ctgctcaatc agctgctcac gaatggccct ggcgcgccct tgtgccaata ggcgcaactc
      841 cgactcctca accgtcattg ccggaaagag ctgttgtcgc agttcgtcgg ccgtcaacac
      901 ccgttcgacg aatttccccc ccgggagctc ttcacgcttc atcggttgct ttcccaactt
      961 ctccgcatag agatcactca ggagttcaaa ctcccgcgag ggcgagggca cagcttgcag
     1021 atttttggtt cccccctgag taaatcgtcg ctgcacctcg gtcacaattt tctgcagagc
     1081 cagggcttcg cggtctcgcg caggatcggc cgcacccgcc acatccaccc gtaacgccgg
     1141 tcgttcctgg agtgccttgg caatcgactc gatctttcgc tgctccacac tctccaggac
     1201 ttcgcttccg gccatgaatt cgatgaactg caagtcttca ccgctgccgc cgacaaggcc
     1261 ccccagcgcc gaaaacggag aggtggccac tttggtgatc agattggcca acgcattcag
     1321 cacgactcgg ccgtagcgaa agtccggttc attcagatca ccgcgcaccg gcatatcgat
     1381 atcgatccgc ccccgtcggt ctttcaacaa ccccacggcc agacgaaccg gcaggctcgt
     1441 ggcatccggg ctgtcggttt tctccccgaa ggtcagctga tcgaccagga ccttgttttc
     1501 ccccacaagt tgctgcttcg acacctgata catgaggtcg agcgagagct tgcccttggt
     1561 gatcggatag ccgacatact tgcccgcgta gggcgacacc gccgtcaggt cgacgccctt
     1621 gaacaggaag gtcagatgcg tatacgtatc ctcgctcagg ggattgacct gcccctgaat
     1681 cttgagcggt gccacttcgt cgactgtccc ggtcagggcc acctccgctt tcgcgatctg
     1741 cttggaggac aggcccttga tcgtaccgct cagattttga atcccggttg tgataaccgg
     1801 ttcgatcgac tcgtcgatga acgtggccga caatttggac aatcggacga cattcacgtc
     1861 gatgggaatc ggagccgtcg ccttcttggg aactgtcgcc tcaaccggct tcgataccgg
     1921 cggctgtgcc ggaccttcag caggagcctt catcacgcgc gaaagattcg tggttccgtc
     1981 tttggccgtc acaaaccgga tcgccggatc gcgccacgca atctccccga tcttcacctt
     2041 cgtcggggcc acctccagcg cgaccttgtt cagccccagt gccgtccagc caaggaactc
     2101 atgggcagac actccgtcgg ccacatgtag actattcagc cccagctgtc ccgtatactg
     2161 gatcatcggt tccgggtcat gttggctgcg atacaccagc tccccgtcca attccaattc
     2221 gccgtctctg atttcaacct gcatgcgacg gtcgagatag ggctgaaagg gccgcagacc
     2281 gatatgcgcc aatttgaggg tgagcgcagt ttgcagggga tccagttgca gcgtgccctt
     2341 gctctcaacg gtgccctgtt gattgagccg aaaaccggcg gccaccggcc acgagccctt
     2401 caacggcaca tggatcccgt tcaccgtcac atgcaggtca tcgattgcca actgcgccgg
     2461 cgtcttcaga ctccgatctt cgaacgccac ctgcgccttg gttacctcaa tcgcctgcac
     2521 gtccaccgac cagggctgcc cggaatccgt cgcaggccgc tgcggctcaa ccgggtcctc
     2581 ccgggaagtc gcaaccggag taaagagtgg tttaaaattc acgacgccgt cctcggcaag
     2641 ccaggcacgg atctctgcac cgctcagcgc gatcgtcccg actcccgcct cccgctttgg
     2701 aaggtccatg cgaatgcctt cgaacgccaa cgccggcacc gtcatgaccg gatcacgctc
     2761 ccccgccgca gacagccgga aatcggacag taggactttt ccctcgttca cctgcaggtt
     2821 gaccggagca gccgacgtat caaagtgata ccgggcatcg acgaggacgg ccccgctcag
     2881 gatgtcgaat cgaaatcgat cgcgcagact cggccagaac gtgttgagtt tgacgtttga
     2941 cagagagaca tggccgtcgg attccagcgg atcgaggtgg accgtcccct cccaggccag
     3001 cgtttccccc tcgtcgaact cggccgagaa ggcataggcg ttttcacccc cgcgccgggt
     3061 gccgaaattg cgcagcgtga tctcaatcgg caccacatcg atcgtcaccg gtttgcgttt
     3121 cgaatcgtcc cggtactcga tgaccccctg ccggatgctc agaagacgaa tctcgaccgg
     3181 cggcacaggg gccttctccg ccttggcatc ggctggtggg gcaggatcgg ccggtgagtc
     3241 ttgagccggc ggcaccagcc ccagcagatt caacgtgcca tcggcccgga tgtggacaag
     3301 gccaaagggg agggttaatc ggatttcgtc gaacagatag gccgaccgca ccaacgaggt
     3361 cccttcgaag tccacgaaga gttcttcgaa ccccagcatg ggcgtctgat ccggctcccg
     3421 aatctcgaag gccgtcaaca tcagcgaaaa ggtaaatgga ttgatccgga cgtcaccgag
     3481 cacgacaggg tgccgaagac gttcggccaa ggcaggaatc gcgtaggtct tgacggcata
     3541 gggcgcgacg acaaaaccga gcagcgcata gagcgcgacg atgccacaga gcacaatgag
     3601 ggctgttcga taccgcgaca agagcgtcat gtcaggaaca cgttgaggaa gtggtgcgcc
     3661 cggttggaat cgaaccaacg accctcagct tagaaggctg atgctctatc cgactgagct
     3721 acgggcgccc ctgtattttc agtcacttaa gagatgatta ctctacagag ttttcgccta
     3781 gtgttcccgg ccggttctca tggaggtatc tgtgaacctt ttgggcagcg tgagtcaagt
     3841 ctcgctcctc gatggcattg taccgtttcc acatcttctc ggacttatgg ccaacaattt
     3901 tcataactgt cgctgtatcc acccctgcgc ttctgagatg agtagaagca cagtggcgca
     3961 tgccatgaaa cctgaagtcc gtgatacctc cctcctcgag ggccgtattg aaagaccggc
     4021 tgccgcggct caatggtcat tccttataca agaagctgtg tcgggatgcc aggctctccc
     4081 tttcccgaca gagccgatgg aggggctcac gttccccgag cgatcgcgca agaacgatcc
     4141 gccgtttgga atctgtcctt cgaccgagtg accgttcctc gttgtagacc tacagacttg
     4201 tcgagcatgc tatggcactc ttccaagaaa gagaagtccc atcccctacg taccgtagct
     4261 catatgtacc caacggtctt attgacctgt gagaatcatt atattcgcaa gaaattgtga
     4321 tcactaaaca tttctgtcat cacattgtct tgacattaga tgaatgtcga gctatcgtaa
     4381 gcaacaacaa gaggtggctt atggcaactc gcgcgcttcg tactgaaaaa ctggatcttc
     4441 gggtgagcgt ctcaaccaag aaaacgctgg aagctgccgc ctctgtctct agtcgtacag
     4501 tgagcgcatt tgtccttgaa agtgccctgg cccgtgcaga tgaagcattg gcagatcgcc
     4561 gaacgttcca tctgagcaag gctaaatgga ctgagttctt agctgccctt gatgctccga
     4621 cccgccccct tcctcagatg caacgtctcc tagctgaacc aggcttcttt gatgcgacac
     4681 ctgcgcctgg cttcaaagaa aagcgttgac taggcgtatc gagaaacttc agccgcatca
     4741 cgcagttgaa gcgttcgact gcggacatga agtcctcaat cgcttcctcc agaagcacgc
     4801 agtacaaaat caattcagtg gtgggtcgca aacctatgtg gggttggtcg ataacattgt
     4861 cgtcggttat tacgcactcg cggtgggatc ggtggaacaa gacaacgctc cggaaagagt
     4921 gaagaagggg ctggcaaaac attccattcc catcatgctg cttgccaggt tagcggtaga
     4981 tcttcactgg cagaaacaag gagtcggtgc tgccctattg aaggacgcta cactgcggac
     5041 gcttcaggcc gccgatattg caggaattcg cgccttggtg gtccatgcca aagataatgt
     5101 agctaaaaag ttctacgagc gtttcgattt ccttccttca ccaagcgatg ctttgcatct
     5161 cttcattctc ctcaaagact gatcttcccc cgatttcgtg gacacgtggt tacgcgactg
     5221 ccgccctcgc ttcaaactcg actggagcgt gatagccgag ggtcgaatgc tgacgctgcc
     5281 gattgtagaa cacctcgatg tattcgaaga tgtcctgctt ggcttcgtct cgggtgacat
     5341 acggtcgctg gtgtaccagc tcacgcttga gcgtcccgaa gaaactttcg acgcacgcgt
     5401 tgtcccagca gttgcctttg cgactcatac tggggatgag gccatactcc gtgagtcgcc
     5461 gctggtagct cgtggcggca tactgggcgg attcaagtgg tcgttgcaac atattctttg
     5521 ttggcctatc agagcaagtg
//
LOCUS       DAORRQ010000134         5505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_500_length_5505_cov_7.955413, whole genome shotgun sequence.
ACCESSION   DAORRQ010000134 DAORRQ010000000
VERSION     DAORRQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_5505_cov_7.955413"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PK224_20365"
     CDS             <1..953
                     /locus_tag="PK224_20365"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRB18160.1"
                     /translation="FQADLSDVRAFRGASVLLAGAGARAAAWPETLAFVSLNPPPRIV
                     AHEVAHVLQYRKTPSPIAERRGLVSRRDPAEAEAGRAAGQFESDAPIRVQARPSVAPM
                     YYTDEEEPDSKEFLEERPPLFVGEEVGSWGSSWPATLIARERLAVSVPEGQRSHAIVE
                     AIALREPIVILHEYGRLWLYRLEWEGLSGRYSRFTNAETIFHKGTNERGDYSVSNVLG
                     RPEVEAFVTEDGGVLSPPGAGRLFSHTGGEFEDPLVSKFQNASAFLDGMGRGLEGAGF
                     EALAERLRRMAALNVVFPAPFAIGAVRGAWWTKCSTSCNC"
     gene            929..2287
                     /locus_tag="PK224_20370"
     CDS             929..2287
                     /locus_tag="PK224_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18161.1"
                     /translation="MLDLVQLLNPQQWTAIEAAARETILLLNDPDGEILAATLGEEFG
                     RNQAAALDGLLQQSLPIFAYEVGKLIGPTIVELLLALVGIEIGPVAMVQKSLAAMKQI
                     PRMAGVVRDLTLVFPNLPNGPTMPSKLIPDEASSSRTITELLSPEGLVSPRQVPRGLP
                     DLSSEESALLARTGNRTEFPDALPKDLADQELAIVKRAEKVSISDGDGKYINEVDLSN
                     GHRWKEQPNGTWCRFSDNPTNCTALVPTPGPVSPGTIVAKAADRESLLERYTAWATNR
                     GADQVEVAILKAISGTGEYPAGTYAVVVGSRNGVVYPGNTPGVRWLTISHVHPGVANE
                     PGYRHPSLVDLAAAEEDTVRLEKLVGGVRKWIHFQTPEGSWNSVKYGYDATIERYFVE
                     IAGEDRLTFERLHDVTDDQLREYDRLDEAGLEQDRINLMNQENSDGSYLKWYAEQFSF
                     MQ"
     gene            2471..3544
                     /locus_tag="PK224_20375"
     CDS             2471..3544
                     /locus_tag="PK224_20375"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HRB18162.1"
                     /translation="MTERLPHAWTGATPSLVHEVLASPGEPLEAGLRAQMESRFGHDF
                     GKVRVHRDGKAADSATAVQARAYTVGHEIVLGSAAAGLGRRGTVSLLGHELSHVVQQE
                     RAFLSSGHPGVLQRSGLEVPNDTQGQASSASNGPENQAAGLSEPDCDTRVADVFLLGA
                     IHCPQRPECCFAQVHDRNNRNLTGIFRADHQLEGKPDCDYGDQKHHDFWLANQWRILE
                     ITTSEMTVMNMCGREETLNVEGIGTVKGESASAVVPGRAPSSTSLPPNIVDHTQGMWG
                     KTHKIHYDDQCNSLHFVPNEPGKEIKVYIWDSAQDSFVNASDPADTRTPGQLERFAGI
                     VLKEHKDGEWQGTNCGDLPRWAL"
     gene            complement(3568..4635)
                     /locus_tag="PK224_20380"
     CDS             complement(3568..4635)
                     /locus_tag="PK224_20380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRB18163.1"
                     /translation="MQGPAGQYPTLQVHPGRRCNLQCLHCYSDSGPAVSEQLEIDLLE
                     GVVADAAAIGYRVMSVSGGEPLLYPGLAQLLRAAHTSGLVTTVTTNGMLLDQRQLNVL
                     GADCDLVAISLDGIPESHNFMRNSTRAFDDMCARLPALRASGIPFGFIFTLTFHNVHE
                     LEWVADFAVEQGASLLQLHPLEPVGRATCRLGESYPDGEENAAAVCEAGRIRELYEGQ
                     LEVQIDLATVPAMLEHPTRVFVREATLCGAQTVADVIAPLVIETSGIVSPLQYGFPRA
                     WSWGNIKERSLPDLAADWLARDYAAFLTLCRLVYEQAVRNDDEPVLNWYEQAYAAAAQ
                     FSPTQVRENLMQTCSSERSSQ"
     assembly_gap    4783..4792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5228..5407)
                     /locus_tag="PK224_20385"
     CDS             complement(5228..5407)
                     /locus_tag="PK224_20385"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HRB18164.1"
                     /translation="MNTVARVRDYSGLVAEFTIYLKEKKSYWLAPIIFVLMALSALAF
                     FLEGSVLAPAIYSVF"
BASE COUNT         1122 a         1536 c         1737 g         1100 t
ORIGIN      
        1 ccttccaggc cgacctctct gacgttcgag cctttcgtgg cgcatccgtt ttgctggcag
       61 gggccggcgc ccgtgcggct gcctggccgg aaacattggc cttcgtgtca ttgaatcctc
      121 ccccgcgcat cgtggcgcac gaagtcgcgc acgtccttca atatcgcaag actccgtcac
      181 ccatcgctga acggcggggt ttggtttccc gtcgcgatcc ggcggaagcc gaagccggtc
      241 gcgccgcagg ccagttcgag tccgatgctc ctattcgggt gcaggcccgt ccgtccgtgg
      301 ctccgatgta ttacaccgac gaagaggaac ccgattcaaa ggagtttctg gaggaacgcc
      361 cgccgctctt tgtgggcgag gaggtgggaa gctgggggag ttcatggccg gcgacgttga
      421 ttgcgcgcga gcgactggcg gtgagtgtgc cggaggggca acgcagccat gccattgtgg
      481 aggccattgc actgcgagag cccatcgtga ttctgcacga gtacggccgt ctctggctct
      541 accggctcga atgggaggga ctctccggcc gctactcccg ttttaccaat gctgagacga
      601 tttttcacaa ggggacgaac gagcgaggag actattcggt ctccaacgtt ctaggccgcc
      661 cggaagtcga agcctttgta acggaggatg gaggggtgct gtcacctccc ggagcaggcc
      721 ggttgttttc ccacaccggc ggggaattcg aggatccgtt ggtgtcgaag tttcagaatg
      781 cgtcggcctt tctcgacggc atgggcaggg gcctggaagg agcgggcttc gaggccttgg
      841 cggaacggct tcgccgtatg gccgccctga atgtggtgtt tccggcaccc ttcgcgatcg
      901 gcgctgtccg gggggcctgg tggacgaaat gctcgacctc gtgcaattgc tgaatccgca
      961 acagtggacg gccatcgagg cggcggctcg ggagacgatc ctgctgctga acgatcccga
     1021 tggcgagata ttagccgcca cgctggggga agagttcggc agaaaccagg cggctgcact
     1081 cgatggcctc ctgcagcaga gcctaccgat tttcgcctat gaggtcggca agttgatcgg
     1141 cccgacgatc gtggaacttc tgctcgcgct ggtcggcatc gaaatcggtc cggtggcgat
     1201 ggtgcagaaa tcgttggcgg cgatgaaaca gatcccgcgc atggcgggcg tagtccgtga
     1261 cctgacactg gtgtttccga atctacccaa tggtccgacg atgccgtcca agctgattcc
     1321 cgacgaggcg tcgagcagcc gaacgatcac tgagttgctc tctccagagg ggcttgtgtc
     1381 gccgagacag gttccaaggg gacttcccga cctgtcatcg gaagagtcgg cgctcttggc
     1441 cagaaccggg aatcggacgg agtttcccga tgcattgccg aaggacctgg ctgatcaaga
     1501 actcgccatc gtgaagcgtg cggagaaggt ttcgatcagc gacggtgacg gcaagtacat
     1561 caacgaggtc gatctgagca acggccatag gtggaaagag caacctaatg ggacctggtg
     1621 ccggttctca gacaacccga ccaactgcac cgcgctggta ccgacgccag gcccagtgtc
     1681 accaggaacg atcgtggcga aggctgcgga tagagaatca ctgctggaac gttacacggc
     1741 atgggccacg aatcgcggcg cggatcaagt tgaggtggcg atactcaagg cgatttctgg
     1801 aacgggtgag tatccggccg ggacctatgc ggttgtggtc ggctcaagga acggtgtggt
     1861 ctatccggga aatacaccgg gagtcaggtg gttgaccatc tcccatgtgc accctggtgt
     1921 ggcgaatgag cctggttatc gccatccttc gctcgtggac ctcgccgcgg ctgaggagga
     1981 tacagtccgg ctcgaaaagc ttgtcggagg cgtcagaaag tggattcatt tccagacgcc
     2041 agaagggagc tggaattcag tgaagtatgg gtatgatgcg accatcgagc ggtatttcgt
     2101 tgagatcgca ggcgaggaca gactcacttt tgagagactg cacgatgtca cagatgatca
     2161 actccgtgag tacgatcgac tcgatgaggc agggttggag caagatcgca tcaatctcat
     2221 gaatcaagag aactccgatg gctcgtatct caagtggtac gcggaacaat tcagcttcat
     2281 gcagtagcga tccggaccga agagaaggtg caagcatggc gacaggaggt cgatgatgat
     2341 acgaagactt caggtttact cgcccagttc ggctgttttg tccacgaaac ggagtgcctc
     2401 gtttgatggc cgtcggggtg atcgatcttg ctcctgcggc aacacctgtc cggattgtcg
     2461 cgccagacag gtgacagaga gacttccgca tgcctggact ggcgcgaccc catccctcgt
     2521 ccatgaggtg cttgcgtcgc cgggggagcc gctagaggcc ggactgcgcg cgcagatgga
     2581 gtcgcgtttc gggcatgatt ttgggaaggt gcgtgtgcat cgcgatggca aggccgcgga
     2641 ttcggccacg gccgtccagg ccagggcgta cacggtcggc catgagattg tgttgggctc
     2701 ggcggcggcc ggtttgggga gaagaggaac cgtatccctc cttggccacg aactgtccca
     2761 cgtggtgcaa caggaaaggg cgttcctgag ctctgggcat cccggtgtgc tgcaacgttc
     2821 cggacttgag gtgccgaacg acacgcaggg acaagcctcg tctgcgtcga atgggcctga
     2881 gaatcaggct gccggtttat ccgaaccgga ttgcgacacc cgcgtggcgg atgtgtttct
     2941 gctcggagcg attcactgtc cccagcgacc ggaatgctgt ttcgcccagg tgcatgatag
     3001 gaacaataga aacctcacgg gcatttttcg tgcagaccat caactggaag gcaaaccgga
     3061 ctgcgactat ggcgaccaga aacaccacga cttctggttg gcgaaccaat ggagaattct
     3121 cgagatcacc acctcggaga tgaccgtgat gaacatgtgc ggacgggagg agaccttgaa
     3181 tgtggagggc atcggtacgg tcaaggggga gtcggcatcg gccgtcgttc ccggtcgtgc
     3241 gccttcatcg acgtcgttgc cgccgaacat cgtcgaccat acccagggta tgtggggcaa
     3301 aacacacaag atccattacg atgaccagtg caactccctc cacttcgtcc ccaatgaacc
     3361 gggtaaagag atcaaggtct acatctggga ttcagcgcag gatagttttg tgaacgcgag
     3421 tgatccggcc gataccagga ctcccggtca actcgagcgg ttcgccggga tcgtgttgaa
     3481 ggagcacaaa gacggcgagt ggcaagggac aaactgcggt gatcttccga ggtgggctct
     3541 gtgagaggga gggatgcgac ccgcctgcta ctgactgctt ctttcagatg aacaggtctg
     3601 catcaggttc tcgcgcacct gcgtggggga aaattgcgcg gccgcggcat acgcctgttc
     3661 gtaccaattc aacaccggct cgtcgtcgtt gcgcaccgcc tgttcataga cgaggcggca
     3721 cagcgtgagg aaggctgcat agtcgcgggc gagccagtct gcggcaagat ccggcaacga
     3781 tcgctccttg atgtttcccc aggaccaggc acgcgggaat ccatactgca acggtgagac
     3841 gatgccgctg gtttcgatca ccagcggggc aatgacgtcg gccacggtct gcgcgccaca
     3901 caatgtcgct tccctcacga agacgcgcgt cggatgctcc agcatcgccg gaaccgttgc
     3961 gagatcgatt tgcacttcca gctggccctc gtacaactcg cggatacgtc cggcctcgca
     4021 gacggccgcc gcattctctt cgccgtcagg ataggattcg cccagtctgc aggtggctct
     4081 gccgaccggt tcaagcggat ggagctgcag aagggaggcg ccctgctcga cggcaaaatc
     4141 tgcgacccac tccaactcgt gtacgttgtg gaacgtgagc gtgaagataa agccgaaggg
     4201 gatgccggag gcccggagtg ccggcaaacg cgcgcacatg tcgtcgaatg cccgcgtgga
     4261 gttccgcatg aagttgtggg attccgggat gccgtcgagg ctgatcgcga ccaggtcgca
     4321 atcagcccct aagacgttca gttgacgctg atcgagaagc atgccgttcg tcgtgacggt
     4381 ggtcacgagc cccgaggtat gggccgcccg aaggagttgg gcaagcccgg gatacaacaa
     4441 gggctcgccg cccgacaccg acataaccct gtatccgatg gcggccgcat cggcgaccac
     4501 tccctccagc agatcgatct ccaactgttc agacaccgcc ggtcccgaat cggaataaca
     4561 atgcaagcat tgcaggttgc atcgtcggcc cggatggacc tgcaatgtcg ggtactgtcc
     4621 ggctggtcct tgcacgatcg gtacttagcc tcgcagcgtg aacttgattt gggcgatgtc
     4681 cggccgcggc gtgccgttga tgaggatatg gagatcacgc agttccttca atttcgccag
     4741 cgtgccgagg ttggccttga agttcgccac gcccgtctgc acnnnnnnnn nngtgccgat
     4801 cacgacttcc ggatgcggcg tgccgacctc atgccaatcg attaccttga cacccttcag
     4861 gcgtttcttg atcgcacttg ccgcagcgct taacttcacg gatccgcgcc cgcttggttg
     4921 ttttgccatc ggagacctcc tttggattgt gatggtcgcg ctcgttcctg agcacgtcgt
     4981 gactagatgg gcgcaccata caacttctta cataaaacga caagcacatc tcaccgcccg
     5041 atcacgatga atacgtgtga tcccggtgag agttaggcgt acgcagctgc aaccgggcag
     5101 gcagtgattc ctgcggaagg tttcggactt acaagtgttg cgatgtgagg caatgcggca
     5161 gcgaggaatg ctggctgtgt cgtggggcaa gcggcgcgcg cccgctctgg gggcgcgcgt
     5221 acaagaatta gaacacggag tagatggctg gcgctaacac gcttccctcg aggaagaacg
     5281 cgagcgcact cagggccatt aagacgaaaa tgatcggcgc cagccaataa gactttttct
     5341 ccttcaagta gatcgtaaac tctgcaacca ggcctgagta gtctctcact cgtgcgactg
     5401 tattcatatg cgcctccttg taaacgattg atgagaacct atcttgaacc gactgccggt
     5461 cgacacatca cgatttgacg gatacgatag atgtctggat tctct
//
LOCUS       DAORRQ010000135         5435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_511_length_5435_cov_8.131413, whole genome shotgun sequence.
ACCESSION   DAORRQ010000135 DAORRQ010000000
VERSION     DAORRQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_511_length_5435_cov_8.131413"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK224_20390"
     CDS             <1..235
                     /locus_tag="PK224_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18165.1"
                     /translation="VHGQLSRITVPTLVMVGAKDGVATPTIAKGIQAQIPGAQLVEFD
                     TGHFMMAEDPDQFRTVLGDFLKQLTRQPAPHGS"
     gene            357..593
                     /locus_tag="PK224_20395"
     CDS             357..593
                     /locus_tag="PK224_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18166.1"
                     /translation="MIAYTHSFIEALRSDTRQAFYDQHKPIAHVMHLMVFLLPLAGVV
                     VRGMVGGVLGLTLFLLCYYLMPYAWFALHDSSRM"
     gene            863..1045
                     /locus_tag="PK224_20400"
     CDS             863..1045
                     /locus_tag="PK224_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18167.1"
                     /translation="MNRHDIEQKLPAMTQAQLVETVKTCLDMIERLENDLAVGTHKLD
                     EMTALVRKLSDVKSVT"
     gene            1150..1374
                     /locus_tag="PK224_20405"
     CDS             1150..1374
                     /locus_tag="PK224_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRB18168.1"
                     /translation="MNHKGQRKKHFTILLALALILLGLPGFARAHDDSKPVVPIMTVT
                     GTGNLAIAPDMAYVTFGIQTVGRLLAEARR"
     gene            1387..1929
                     /locus_tag="PK224_20410"
     CDS             1387..1929
                     /locus_tag="PK224_20410"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRB18169.1"
                     /translation="MQKVIDRLTELHIDKARIQTSAFSVSPQYKPPPKHNVDALPMAP
                     EIIGYLVSNQLTVEVFNPEKVAAVIEQALAAGANHFQGLQWALKDEQQATRGAMKQAV
                     ANAREKAVTLSESLKLKLVRLISVNEAGQVVRPMSHASRSMMAMDAGGGLPIFGGELK
                     VEANVTLVYEIAQNDSVSTP"
     assembly_gap    2148..2170
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2350..2763
                     /locus_tag="PK224_20415"
     CDS             2350..2763
                     /locus_tag="PK224_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18170.1"
                     /translation="MPVSRHIIGISLGVVLFAGNGLAAEPAEVEKFVNARIEIGEMMT
                     NYFKGGESYGSGQRPSPEKMKEMGDDINAKLTTLLSKHGLTLEEYRARSKDVFADDAA
                     VKGYLAQHPDLKTRYEALPLNRMSGGGGGSTGRGY"
     gene            complement(2887..3336)
                     /locus_tag="PK224_20420"
     CDS             complement(2887..3336)
                     /locus_tag="PK224_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18171.1"
                     /translation="MTHMTKRLATMATMLAFVASVAGPAMTAAATPEKTSIEIKVRVS
                     EKGFLDEKGRPYGAKRALQVPKDTLVKITFVFGEDMTSLAVGDTHQITIRAEDGWKQE
                     TGSVWVMNRESSVSFLAGEKNRTQYRAYCILDCIGMEHLTNLLIQVV"
     gene            complement(3663..>4218)
                     /locus_tag="PK224_20425"
     CDS             complement(3663..>4218)
                     /locus_tag="PK224_20425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRB18172.1"
                     /translation="VTTHQDEKGRKTIFDVLPPELKSLHAVGRLDQATSGLLLLTNDS
                     TLSSFLTDPKQQVPRLYLVTVRGEVSDETGRAALDGVDDQGERLHCASVTIQKRSGRE
                     SHLAVTLTEGKNREIRRLFKALGHEVTRLRRIQYGPFTLGDLQPGAWRELPIDAARAQ
                     LQLPPAHRARVPQAKPDLLDKRAP"
     assembly_gap    4219..4228
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4229..4474)
                     /locus_tag="PK224_20430"
     CDS             complement(<4229..4474)
                     /locus_tag="PK224_20430"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRB18173.1"
                     /translation="MPRAKASSAKRVTLDRLLSKLGIASRSQAQEWIRAGRVRINQRL
                     VRMPDTWIDWPGDAVTIDDRPVQQRPPQFILFHKPKGV"
     assembly_gap    4560..4591
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4787..5200
                     /locus_tag="PK224_20435"
     CDS             4787..5200
                     /locus_tag="PK224_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18174.1"
                     /translation="MVIHPARLMSGILSVVVLCGGTGWAAEPAEVEKFVNARVEVGEL
                     MTSYFQGWERYGEGQRPSPERMEEMTVEINAKLEPLLAKYDLTVESYRERIKDVLADE
                     VAVKGYLSQHPDVKKRYDALPFDRMRRDGGSSRGY"
BASE COUNT         1068 a         1463 c         1705 g         1134 t
ORIGIN      
        1 cgtgcatggc cagctcagcc gcatcactgt gccgactctg gttatggtcg gcgccaagga
       61 tggtgtcgcc acacctacca tcgccaaggg aattcaggcg cagatccccg gggcgcagct
      121 ggtggaattc gataccggcc attttatgat ggcggaagac cccgaccagt ttcgcaccgt
      181 gctgggcgac ttcttgaagc agctcacacg ccaacccgcc ccgcatggct cttgacgcca
      241 tgcctcattg agcgtagcgt gatgcgctag gtagccgctc tctcggtgat cgatgcctca
      301 ggctaccgct gccggcatat ccgttcttcc tgttcgatgg acgaggaggt gcggtgatga
      361 ttgcttacac tcattccttc atcgaggcgc ttaggagtga cacccgccaa gcgttttatg
      421 accaacacaa gcccatcgcg cacgtgatgc acctgatggt gttcctgctc cctctcgccg
      481 gggtcgtcgt cagagggatg gtcggtgggg tgttgggcct cacattattt ctcctgtgtt
      541 actacctcat gccctatgca tggttcgcgt tgcacgacag cagtcgaatg taagccgagc
      601 gatctcgctc gactcatgcc gtcatcgccc gagtaatcgc gcgtccgtta ctcctgcctt
      661 ctagtaccgg cccccatacc tttcctgtgc ccctatcaag attcaccgat agggagcatt
      721 gctgaatgcg caagcttgtc gtcgagggtt gtggcgtagt ccattcagct gacagtgacg
      781 ttcagggacc gcgcgcgccg cagttgaaaa cgcctcttgg atacgtcaca ttgctccatg
      841 aaaacggcat gggagaataa gaatgaacag gcatgacatc gaacagaagt tgccggcgat
      901 gacgcaggcc caactcgtcg aaacggtgaa gacctgcttg gacatgattg agcggttgga
      961 gaatgacctg gccgtcggga cgcacaagtt ggatgaaatg acggcgctcg tgcgcaagtt
     1021 gtcggacgtg aaatccgtca cctaatgccg cctcacccat catcggcctc aacattcgtt
     1081 ttgcttcgtc ctgtggttga tccggtgtgg aaccgaggat cgtctgaaca gaaacataac
     1141 gaggatgaga tgaatcataa ggggcagagg aaaaaacact tcacgatcct actcgcactg
     1201 gctctgatcc tgctgggcct ccctggattt gcgcgagccc acgacgactc gaagccggtc
     1261 gtcccgatta tgacggtcac cggcacgggg aatttggcaa ttgcgccgga catggcctat
     1321 gtgacgttcg ggatacagac ggtcggcaga ttacttgcgg aggcccggcg gtagaacagc
     1381 aacgtcatgc agaaagtgat cgatcgactg accgagctgc acatcgataa ggcgcgcatt
     1441 cagacgtcgg cattcagtgt gtcgccacaa tataaaccgc cgcccaaaca caacgtcgat
     1501 gcgcttccga tggcaccgga gatcatcggc tatctggtga gtaatcagct gacggtggag
     1561 gtcttcaatc cggagaaggt cgccgccgtg atcgagcagg ccttggcggc tggggcgaat
     1621 cacttccagg gtttgcagtg ggcgctgaag gacgaacaac aagcgacacg tggcgcgatg
     1681 aagcaggccg tggcgaacgc acgcgagaaa gccgtgacgc tgagcgagtc gttgaaatta
     1741 aaactggtgc ggttgatcag cgtgaatgaa gcaggacagg tggtgcggcc gatgtcgcac
     1801 gcgtcacgct cgatgatggc gatggatgcc ggcggcgggc ttcccatctt tggcggagag
     1861 ctgaaggttg aggccaacgt gactctggtc tacgagattg cacagaacga cagcgtctcg
     1921 accccgtaac atggctcagc tcagaccgtc gttccgaaca gcgccccgac gcctgccgtc
     1981 gccatcgcca gtgcacccca gaaggtgacg cggatggcac ctcacagtat atgggctccg
     2041 cctacgtatg ctgccaatcc accgagaagc gtgaggaaaa ccagcgagag tccggagacc
     2101 catgggatga tacctgactc gaggaccacg gtcacggcga gtagcggnnn nnnnnnnnnn
     2161 nnnnnnnnnn tccacatggc tacagggcgc tggtttctat gttcaggggt cggctcttcg
     2221 cacatcctgc cactcttcgt ggcctgccag gtggatcgcc tgacaagtgc aggtcgtccg
     2281 gtcgagatgg tatgatgccg aaaggcagcc tatatgatta gattcatact ctgggaggac
     2341 attatgacca tgcctgtatc gcgtcacatc atcgggatct cattgggcgt cgtgctgttc
     2401 gcaggaaatg gcctggcggc ggaaccggcc gaggtggaga agtttgtgaa cgcccgcatc
     2461 gagatcggcg agatgatgac gaattatttc aagggcgggg agtcgtatgg atcgggtcag
     2521 cggccgtcgc cggagaaaat gaaggagatg ggcgacgaca tcaacgcgaa actgaccacg
     2581 ctcttgtcca agcatggctt gacgctggag gagtaccgcg cgcgcagcaa agacgtgttc
     2641 gccgatgacg cggcggtgaa aggatatctc gcccagcatc cggatctgaa gacccggtat
     2701 gaggcgctgc cgttgaatcg gatgagtggg ggtggaggag gcagcaccgg acggggctac
     2761 tagcaggcgg gtgaatcctc accactctgc gtgctcacaa attcgaccgc tcggcgtatt
     2821 gcatcagtac gccgagcgtt ttcatttgac cgcggctttg ccggacaggc tagttgacca
     2881 gcctggttag acgacttgga tcagcagatt ggtgagatgc tccatgccga tgcagtcgag
     2941 gatgcagtag gcgcgatatt gagttcggtt cttttccccg gccagaaaac tcacgctcga
     3001 ttcccggttc atcacccaga ctgagccggt ctcctgcttc cagccgtctt cggcccggat
     3061 cgtgatctgg tgcgtgtctc ccacggccag gctggtcatg tcttcaccga acacgaaggt
     3121 aatcttcacc agcgtatctt tcggcacctg cagggcgcgc ttcgctccgt agggtcggcc
     3181 cttctcatcc aggaatccct tttcactgac tcgaaccttg atctcgatcg aggttttttc
     3241 cggggtggcc gcagcggtca tcgccggtcc ggcgacgctt gcgacaaacg ccagcatggt
     3301 ggccatggtt gccaagcgct ttgtcatgtg agtcattgct ctcgtgctcc tctatttgag
     3361 accgcctgcg atcgcaggtg tgccgttccc ctgtcatagg gtggctgcga gtcttgctgg
     3421 tggtggacta cagctggggg cgactcttac gacaatgcgg aaggtcacgc tccgaaattt
     3481 cgtgtgctgt attcaccata gcacaggcaa gcaggttggc aagttctttc acccgctccg
     3541 gcaaagagaa cgacaggctt gatggaaaaa acgtagggag tcagcgacgg cgcccacttt
     3601 tcagtgagtt gtcggccctg gcctggaaag atttcagctg acgtgtaggg gggagcgggc
     3661 gctcagggtg cgcgcttgtc gagcaggtct ggtttcgcct gtggtacccg tgccctatga
     3721 gccggcggca actggagttg cgccctggct gcgtcgatcg ggagttcgcg ccaggcgccc
     3781 ggttggagat cgccgagggt gaaggggccg tattgaatcc gcctcaatct ggtcacctca
     3841 tgtcccagcg ccttgaagag ccgccggatc tcacgattct tgccttcggt caacgtgacg
     3901 gccagatggg attcccggcc tgagcgtttc tgaatggtga cggaggcgca gtgcaaccgc
     3961 tcgccctgat cgtccacccc gtcaagcgct gcccggcctg tctcgtccga gacctcacca
     4021 cgcaccgtga ctagatagag gcgcggcacc tgctgtttcg gatcggtcag gaagctggag
     4081 agcgtcgagt cgttggtgag caggaggaga ccactcgtcg cctgatccaa gcgaccgact
     4141 gcgtgcaggc ttttcagctc aggaggaagt acatcgaaga tggtctttcg acccttctcg
     4201 tcctgatggg tcgtgactnn nnnnnnnnta cacctttggg tttatggaag agaatgaatt
     4261 gcggagggcg ctgctggacc ggtcgatcgt cgatggtgac agcatcgccg ggccagtcga
     4321 tccaggtgtc cggcatgcgt accaggcgtt gattgatgcg gactcgtccg gccctgatcc
     4381 attcctgtgc ctggctccgg ctggcaatgc cgagtttgga gagcagtcga tccagcgtca
     4441 cacgtttcgc gctgcttgcc ttcgcgcgtg gcatgctccg cgccggcttt cggggaggag
     4501 gcaccctgtc ggacgacgcc ggatctcgtt gtgtgaagtg cggtttgccg gtcatgctcn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggccaagcg gagggccacg tctggatgcg
     4621 gtcgcatggt gtgactgtac actgccgagt gcggaaagag aagtcgatcg atgacagctg
     4681 ccgtggagcg tggggccggg atcagttgga tgtatgtcgc atacctgtga tggtatgatg
     4741 gctgcggctt tgcagcgaac gagggaccat tctctgagag ggcaccatgg tgatccatcc
     4801 ggcgcgtctg atgagcggca tcctgtcggt ggtggtcctg tgcgggggaa ccggttgggc
     4861 tgccgagccg gcagaggtgg agaagtttgt gaacgcgcgg gtcgaggtcg gtgagctgat
     4921 gaccagctac tttcaggggt gggagcgata tggagagggg cagcgtccgt cgcccgagcg
     4981 gatggaggaa atgacggtcg agatcaatgc caagctcgag ccgctcctgg ctaaatacga
     5041 tctgacggtt gagagctatc gtgagcggat caaagacgta ttggccgatg aagtcgccgt
     5101 caaaggctat ctctcccagc accccgacgt gaagaagcgg tatgacgctc tgccctttga
     5161 tcgtatgagg cgtgatggag gcagcagccg aggttattag cagaagatgg cccgaggctt
     5221 ctggaacgtg tatctgaacg cttgtgatgg ggcaaacatg ctgaaatacg tggtgctggt
     5281 gatgttctcg acgctgcttt tttgcaacgt ggcgtcggcg gaatggattc tggtcggcgg
     5341 cacgcacaag tatgacggct acgtggatgt ggccacggtc ggtccgacgg gcaagaccgt
     5401 gacgctctgg accctgaagg acttcaaagt ggatc
//
LOCUS       DAORRQ010000136         5335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_519_length_5335_cov_16.219318, whole genome shotgun sequence.
ACCESSION   DAORRQ010000136 DAORRQ010000000
VERSION     DAORRQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_5335_cov_16.219318"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..2113)
                     /locus_tag="PK224_20440"
     CDS             complement(71..2113)
                     /locus_tag="PK224_20440"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRB18175.1"
                     /translation="MLTLSRFSNPKCLLVLLVHILLMGIANYAAFWLRFDGDLPDWAM
                     DIFLATVPLLVCVRLLMFIPFRLYGTVWQYTSLWDLGNIVGATLSGSVVFFLVTRYGL
                     GQTAYPRTVYLIDALLLIVLTGGMRVARRVGYPSSSNSDTKRVLIYGAGDAGERLVRE
                     LRQNRAFGYQPVGFIDDNRRKSGQRIHGVKVLGTRAQLPGIMERVKPDTILIAMPSIG
                     AGAIREIAKALLAYNVSIKILPDLSVLLDGKSEVNQLRNLSVEDLLHRPRVDLDRSVT
                     AALLKGKRVLVTGAGGSIGSELCRQIATYSPESLILFERYENGLFAIHSELAHRPGPP
                     HIYPVIGDVTDRERLQSAMEAYEPQIVFHAAAHKHVPLMEYNPCEAVKNNVAGTRTVA
                     TLAEEFNVERFIMISTDKAVRPSSVMGATKRVAELIIQDMARTSKTNFVTVRFGNVLG
                     SNGSVVPHFLQQIKNGGPVTVTHPDVQRYFMLIPEAVELVLQSASLANPGAVYVLEMG
                     EQVRLQDLARHLIRLSGFVPDQEIAISFTGLRPGEKLAEELVGPDETVTASPLKEILS
                     VRLDGVPARDWLLPTIAALEEMAYLQNAGEVLAKISQLVPSFRPSQRNSVLALERDWS
                     DGEGENEFANHEPRGGVDEKNYSPGTVRSGARRRVRRDIGSGAVASPTASVPDRFE"
     gene            complement(2212..2841)
                     /locus_tag="PK224_20445"
     CDS             complement(2212..2841)
                     /locus_tag="PK224_20445"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRB18176.1"
                     /translation="MSVKPIQSAHGWRLLGYGVGLAGAGGLLSWLWLIWLVAHAEGVP
                     PAEVGKVDVVVALAGSPDRTVYAQTLVAQGLAPDRMSTLLDPFCLRAQGSRSACATAV
                     RNTIDEAVALRRIFARERFTKVIVVTSRYHLARARVVFDTIFAGGDTAVHVVSPPGDR
                     ISGPRIRREALSYLPSLFGAVMARMVPAVYEWLVRNQPVCPDPGVPPIE"
     gene            complement(2871..3980)
                     /locus_tag="PK224_20450"
     CDS             complement(2871..3980)
                     /locus_tag="PK224_20450"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRB18177.1"
                     /translation="MPIIRPTLPSLEDIFAIMQTGWETGIVTVGPIVRSLEEQACRVT
                     GARYAIALSSCTAGLMLVPQALGLRPGTEVIVPSFTFAATAQALLWNRLVPVFCDCLP
                     GTCTLDPADVERNITPNTGAICGVSVYGLPPDVDALLEIGRRKGIPVYFDSAQGLGAT
                     YKGQSLGQFGVCEVFSLSPTKVVTAIEAGILTTNDAVIAERIRSMRDYGKDLVKGEEM
                     VHLGLSARMSELHASVGLLGLQRVHDLVKARTERIAVYRDRLGSLPGCWVQEFPYDRS
                     TSGNYFVLFIGEQAKRSRDQVYEDLKKAGIQTKRYFYPPVHAQAIFQQYPMKLSAQLT
                     QTKKASEEGLALPLYSHMTDQEIDAVCAEVKQLLA"
     gene            complement(4266..4745)
                     /locus_tag="PK224_20455"
     CDS             complement(4266..4745)
                     /locus_tag="PK224_20455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRB18178.1"
                     /translation="MNVTDKKHALRMFTNGVYILTSRSDEGFGAATVTWVSQASFTPP
                     LLTVAVRPTNSVFRCLAKSSVAALHVLSHEQTEMAKKFFTPTAVQDGTINGEPFSEGT
                     TLAPILSNAPAYLECAVRQIVDQGSDHAVVILEVLEAVCRERVKPLTMADTPWEYGG"
     gene            complement(4785..>5335)
                     /locus_tag="PK224_20460"
     CDS             complement(4785..>5335)
                     /locus_tag="PK224_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18179.1"
                     /translation="SAQGRRVGTSLWARGLAGCNTGAARTLSLDVYEDNTRAAAWYRS
                     LGLEPVAQRIVMAVPIPSVSPTADQAWSSTFLGPADHDYSQYGFSQFTLNTRRSTYTI
                     GRLGTDFFRIASSVLLDDETALAALHRLDPRRRLLGFDAPDRWEAAAGRGAVLLGRTI
                     RLTIPIEQLAARLGKTLSEAGG"
BASE COUNT         1184 a         1613 c         1513 g         1025 t
ORIGIN      
        1 ttttccgtct cccccgactt ttcgctcaac ggtgaggtca tcttcgccgt agacctggat
       61 acgaatgata tcattcgaac cgatccggta cacttgcggt tggggaagcg acggcgccgc
      121 ttccgatgtc acgccggacc cgacggcgag caccagaccg aacagtccca ggagaataat
      181 tcttttcatc tacaccacct cgtggttcgt ggttcgcgaa ttcattttcg ccctcaccgt
      241 cagaccaatc acgctcaagc gccaacaccg aattccgttg cgaaggtcta aaggagggca
      301 ccaattggct gatcttggca aggacctcgc ccgcattctg aaggtaggcc atctcttcca
      361 aggcagcgat cgtaggtaac aaccagtccc gtgcaggaac accatccagg cgtacactca
      421 aaatttcctt caagggagat gctgtaaccg tctcatccgg acccaccagc tcttcggcca
      481 gtttttctcc cggccggagt ccggtgaaag aaatcgcaat ttcctgatca ggcacgaagc
      541 ctgagagccg gatcagatgc cgcgcaagat cctgaagcct gacctgctct cccatctcaa
      601 gcacataaac agctcccgga ttcgccaacg aggctgactg caggactaat tcaaccgcct
      661 caggaatgag catgaaatac cgttgcacat ccggatgggt cacggtcacc ggccctccat
      721 tcttgatctg ttggaggaag tgaggcacaa cgctgccatt gctacccaac acattgccga
      781 atcttaccgt cacaaagttc gtcttgctgg tccgtgccat gtcttgaata atcaactctg
      841 ccacacgttt ggtggcaccc atgacgctgg aagggcgcac agccttgtcg gtcgagatca
      901 tgatgaagcg ttcaacattg aactcctccg caagcgtggc cacggttcgg gtgccggcca
      961 cgttgttctt gacggcttcg caaggattgt actccatcaa cgggacatgt ttatgcgcgg
     1021 cggcatggaa gacaatctgt ggttcatagg cttccatcgc agactgtaac cgttccctgt
     1081 ccgtcacgtc cccgatcacg gggtaaatat gaggaggacc ggggcgatgg gccaactcgc
     1141 tgtgaatcgc gaacaatccg ttttcatatc gctcaaagag tatgagggat tcgggtgagt
     1201 atgtagcgat ctgccggcac agctcggacc cgatggaacc gcctgccccg gtcaccaaca
     1261 cgcgtttgcc cttcaacagg gccgcggtta cggaacgatc gagatccacc cggggccggt
     1321 ggaggaggtc ttcaaccgac aaattcctga gctgattcac ctcggatttt ccatctagca
     1381 acacgcttag gtccggcaga atcttgatgg agacattgta ggccagcaat gctttggcga
     1441 tttctcggat ggcgccagcc ccgatactgg gcattgcgat cagaatggtg tcgggcttga
     1501 cgcgttccat gatgccggga agttgcgccc gcgtgcccaa aaccttgacg ccatggatac
     1561 gctggccgct cttccgccgg ttgtcgtcga tgaagcccac cggttgatac ccgaaagccc
     1621 tgttctgtcg aagttcgcgc accagccgtt ctcctgcatc gccggcaccg tagatcaaca
     1681 cgcgcttcgt gtcgctattg gaggatgagg gatatcccac acgtctggcc acccgcatcc
     1741 cgccggtaag gacgatcaac agcaacgcat cgatcagata gaccgtccgc ggatacgccg
     1801 tctgcccaag tccataccgt gtcaccagaa aaaacacgac gctgccgctc aaggtcgcgc
     1861 cgacgatatt gccgaggtcc cagaggctgg tgtactgcca gaccgtgcca tacagcctga
     1921 acggaatgaa catcaggagg cgcacgcaga cgaggagcgg cacggtggcc agaaagatgt
     1981 ccatcgccca atccggaaga tctccgtcga agcggagcca gaacgccgca tagttggcga
     2041 ttcccatcag gaggatgtgc accaacagca cgagcaggca cttcggatta gagaatcgag
     2101 acaatgtcaa catggtaata gaccgatcag ttgaaggtga ataaaccgtc ggagcgggcc
     2161 gacacaggag cgaaagcccc ggtgacccgc agagggctga agaggaatcg attattcgat
     2221 gggaggaacg ccgggatccg gacagaccgg ctgattgcgc accagccatt catagactgc
     2281 cggaaccata cgagccatga cggctccgaa caggctggga agatatgaca acgcttctcg
     2341 tctgatgcgt gggccgctga tccggtcacc gggcggagac accacatgaa ccgccgtgtc
     2401 gccgccggcg aagatggtgt cgaacacgac cctcgccctc gcgaggtggt atctggatgt
     2461 caccacgatc accttcgtga accgctcgcg ggcaaagatg cgtcgtaacg ccacggcttc
     2521 atcgatggtg ttgcgcacgg cggtcgcaca ggccgaccgg gaaccttgcg ctcgcaagca
     2581 gaacggatcg agcaacgtgg acatgcggtc cggcgcaagc ccctgtgcaa cgagagtctg
     2641 ggcatagact gttcgatcgg gactccctgc cagcgccacc accacgtcca ccttgccgac
     2701 ctctgcagga ggaacccctt cggcatgggc cacgagccag atcaaccaaa gccatgacaa
     2761 caatcccccg gcacccgcca ggccgactcc gtagcctagc agcctccagc catgggccga
     2821 ttgaatgggc ttcacggaca tggcgagacc ggctcccact atccgtcctc tcaggccaac
     2881 aattgcttga cctccgcaca gaccgcatcg atttcctgat cggtcatatg ggagtacagc
     2941 gggagagcca acccctcctc gctcgccttc ttggtctgcg tcagctgcgc actcagtttc
     3001 atcggatatt gctggaagat cgcctgcgca tggaccggcg ggtaaaagta ccgcttcgtc
     3061 tgaatgccgg cctttttgag gtcctcatag acttggtctc gactgcgttt ggcctgctct
     3121 ccgatgaaga gcacaaaata gtttccgctg gtgcttcgat cgtacggaaa ctcttgcacc
     3181 caacagccag gcaagcttcc caaccgatct cgatacaccg cgatacgttc agtcctggcc
     3241 ttcacgagat catgcacccg ttgaagtccg agaagcccga ccgatgcatg aagttcactc
     3301 atcctagccg acaggcccag atggaccatt tcttctccct tcaccagatc ctttccgtag
     3361 tcacgcatgg accgtatgcg ctcggcgatg acggcgtcgt tcgtcgtcaa tataccggct
     3421 tcaatggccg tcaccacttt cgttggactg agcgaaaaca cttcgcaaac cccaaactgc
     3481 ccgagcgact gtcccttgta cgtcgccccc aacccttgag cgctgtcgaa atagacgggg
     3541 atcccctttc tgcggccgat ctccaacaag gcatcaacgt ccggcggcaa cccgtacacc
     3601 gagaccccgc aaatggcccc ggtgttgggg gtgatgttgc gttccacatc cgcaggatcc
     3661 agggtgcagg tccccggaag gcaatcgcaa aagaccggca cgagacgatt ccagagcagc
     3721 gcttgggcag tggccgcaaa ggtgaaggac ggcacgatga cttcggtgcc aggcctgagc
     3781 ccgagcgctt gcggcaccag catgaggccg gcggtgcagg aggacagcgc gatcgcgtag
     3841 cgagccccgg tgacacgaca ggcctgctct tccagacttc gcacaatggg gccgacggtc
     3901 acgattccgg tttcccagcc tgtctgcatt atggcgaaaa tgtcttccaa tgaagggagc
     3961 gtgggtctga tgatcggcat tgtggtcacg gtatcctccg taatgtaggt gaagcaggta
     4021 tcgaatgttg ctgggcgaag attccgaata gtcgggcgaa gcacattcaa cagaggcgaa
     4081 ggtcagcgga tccgtcgcgc gactcgtgaa gaggcagaga caggacttcg tatttcagaa
     4141 tggagccgag gaggcccact caatctagaa tttctggaga gattcctgcg gtaaggctct
     4201 gcgaaaccgg ggaaagacat cgagcacccg attgggtgcc gacaaagagg cgcgagactc
     4261 cgtattcaac cgccatactc ccagggtgtg tcggccatcg tcagtggttt gacccgttcc
     4321 cggcagactg cctcgaggac ttccagaata accacggcat gatcactacc ctggtcgaca
     4381 atctggcgga cggcacattc cagatacgca ggcgcattac tgagaatggg agcgagggtg
     4441 gtgccttcgg aaaagggctc accgttgatc gttccgtctt gaacggccgt cggcgtgaag
     4501 aacttttttg ccatctcggt ctgttcgtgg ctcagcacgt gaagagccgc cacggaactt
     4561 ttggcaagac agcgaaacac gctattggtc ggacgaacgg cgactgtcag caacggcggg
     4621 gtaaaggacg cctgcgacac ccatgtgacg gtcgcggcgc cgaaaccttc atcgctgcgg
     4681 gaggtcagaa tatagacccc gttggtgaac atgcgtagcg cgtgtttctt atcggtgaca
     4741 ttcatgcgcg atctccacaa caaactgaag ccatctcgac cgactcaacc ccctgcctcc
     4801 gacagcgtct ttccgagacg cgcggccagc tgttctatgg gtatcgtgag tcgtatcgtt
     4861 cgcccaagga gcaccgcccc tcgtccggct gccgcttccc atcggtccgg cgcatcgaaa
     4921 ccgagcaacc ggcgtcgagg gtccaagcga tggagcgcgg ccaaggccgt ttcatcatcc
     4981 aacagcaccg atgaggcgat acggaaaaag tcggtcccca accgcccgat cgtataggtg
     5041 gatcgccggg tgttgagcgt gaactgggaa aatccgtatt gactatagtc gtggtctgca
     5101 gggccgagaa aggtcgatga ccaggcttgg tccgcggtgg gcgacaccga aggaatcggc
     5161 actgccatta cgatgcgctg cgcgaccggc tccaggccca atgagcgata ccaggcggcg
     5221 gctcgcgtat tgtcctcgta aacgtccagc gaaagggtgc gcgccgcgcc ggtgttgcat
     5281 ccggcaagcc cgcgagccca caacgaggtg ccgacccgac ggccctgtgc cgacg
//
LOCUS       DAORRQ010000137         5286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_525_length_5286_cov_375.111260, whole genome shotgun sequence.
ACCESSION   DAORRQ010000137 DAORRQ010000000
VERSION     DAORRQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_525_length_5286_cov_375.111260"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PK224_20465"
     CDS             complement(<1..335)
                     /locus_tag="PK224_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18180.1"
                     /translation="MLYNWLYPLHTQFSFLNVFRYQSFRIIYAAVTAFLIAFVMAPWV
                     IRKLQEIKLGQQIRDDGPKRHLAKSGTPTMGGILIIFAVVLSTLLWADMTNLSLARRA
                     IRSGAVRPE"
     gene            complement(400..1863)
                     /gene="murF"
                     /locus_tag="PK224_20470"
     CDS             complement(400..1863)
                     /gene="murF"
                     /locus_tag="PK224_20470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRB18181.1"
                     /translation="MREHGNRGVMALFTVEEICEVLSVRPPAGVTPQDLKQRIRRVVT
                     DSRLVRKGDLFIAFQGERFDAHTFVPKVLAQGAVCAIVQEDYRLPPAPKRTGVPMLLG
                     VRDTLEAYQRLATHYRNRFPIPVIAITGSNGKTTTKEMVAQVVAQRWKTLKTEGNLNN
                     RIGVPQTLFQLAPRHQAAVIEMGVDQQGQTTRLCEIARPTIGVITNIGPDHLEFFGSM
                     EGSAQAKAELLDHLPQDGAVVLNADDEYFDYLAARAQCRVVAFGVSPKAAIRAANIRV
                     DEKGGTVFGLVVPGKSRQTEIRIRTQGQHNVSNALAAAAVGYALGLSGTAIAEGLAKF
                     RPAAMRSQISFSHGVQVVNDCYNANPASMKAAVQLLAELGRGKRSIAALGDMLELGTD
                     SKRMHREVGAFLAAQGIGHLLACGVLGRELAEGARQAGMASDSITELPDAAAAAVALA
                     RMVRQGDVVLVKASRGMRMEQVVEAVTGMRRVARKAC"
     gene            complement(1876..3384)
                     /locus_tag="PK224_20475"
     CDS             complement(1876..3384)
                     /locus_tag="PK224_20475"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRB18182.1"
                     /translation="MTLDELIGPIHGRLGVLERSGDQRVTIADLTDDSRKVGPGSLFV
                     AVQGERVDGHQFLDPVVAAGAAAVVVQRSGDVGAISCVRVQDSRAALGILGSRFYGDP
                     SSSLRMIGVTGTNGKTTTTYVVKTMLEAARRRVGLIGTVAYLVGKESIPASHTTPGAL
                     ELQKLFARMREARLDTVVMEVSSHALALDRTAGSEFDVAVFTNLTQDHLDFHLDMERY
                     FQAKLKLFVELGRAGAVKPRKRAIINADDPWGARIRSACTVPVWTYGLHQQADIYAEG
                     VRLSPAGTSFTLRSPVGTCPIKSRLVGEHNVSNLLAAIGVVLHEGLTLEQVRSAVETV
                     TNVPGRFELVEAGQDFSVVVDYAHTEDALVRLLTAAQALRTGRIITVFGCGGDRDRTK
                     RPKMGRAAVQYSDVVILTSDNPRTEDPAAILREVEVGVKEALADRGHVRYRMVADRRG
                     AIEAAIREAKPGDMVLIAGKGHEDYQIVGTTKHHFDDREVARDTIGALRSGA"
     gene            complement(3386..5104)
                     /locus_tag="PK224_20480"
     CDS             complement(3386..5104)
                     /locus_tag="PK224_20480"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRB18183.1"
                     /translation="MASTPFRSRRIVVACGLVVAFMLVIVRLVNLQVMQSAELTVKAD
                     RQHQKNVTLEGARGTIYDRNSKVLAMNMDVPSVFGVPASLGNPGATARNLSPILHVKA
                     TELEKKLKQEKHFVWLARKLEPEQGRRLERLALDGVGVVMEGRRFYPKGPLLSHVLGF
                     AGMDDRGLEGVELRYEQYLRGEKRAVVLQRDALGRAVFPKGLNEEGAAAGHSLTLTVD
                     EVIQYIAEKELDEAVSRSNAKSGTIIVMDPKTGAVLAMAVSPRFDPNTVGALAPDRWR
                     NRALTDTYEPGSTMKTVIAAAALEEKVMTPGSMIYGENGQLSIANTVIHDHEKTGWMT
                     FAQMIQKSSNIGAAKVGMALGEWRVFDYLKDFGFGDKTGIDLPGEAVGLLRGPRQWGK
                     RSLASISMGQEVGVTPLQMVTAVSAIANGGVLMKPFVVSEIRNAKGQLVAQTMPQAKR
                     RVISADTARTLTTLMEGVVTNGTGGKAAIPGFRVAGKTGTAQKVDPRTGAYSSTLLVG
                     SFLGYVPAEDPRLAMIVVIDEPRGEGWGGVVAAPVFRRVGEQVLNYLGVAVDEPVKLA
                     MAGLES"
     gene            complement(5104..>5286)
                     /locus_tag="PK224_20485"
     CDS             complement(5104..>5286)
                     /locus_tag="PK224_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18184.1"
                     /translation="ISGLTAPERIARLASDKLGMMQPEKGQVVVINIEPEAPANPVVA
                     EGEVRIAKNIVTRRAR"
BASE COUNT         1019 a         1851 c         1403 g         1013 t
ORIGIN      
        1 atctccggcc tcacggcacc tgagcggatt gcgcgtctgg ccagcgacaa gttggtcatg
       61 tcggcccaca gcaacgtgga cagcaccacc gcgaagataa tgagtatccc acccatggtc
      121 ggcgtgccgc ttttggccaa atgccgcttc ggcccatcgt cgcgaatctg ctgaccgagc
      181 ttaatctcct gcaatttgcg gatgacccag ggagccatca caaacgcgat caaaaacgcc
      241 gtgaccgccg catagataat ccggaagctc tggtaccgaa agacgttcag gaatgaaaac
      301 tgtgtgtgaa gggggtatag ccagttgtat aacatcctac ggcatctttg cccgccatgg
      361 atcagcgggc cctaaagttc ctctgtatgt gcctgccgac tagcaggcct ttcgcgcgac
      421 ccgtcgcatt cccgtcacgg cttccacgac ctgctccatg cgcatcccgc gcgacgcctt
      481 gaccagcacc acatcgcctt gccggaccat ccgtgccagg gcaacggctg ccgccgccgc
      541 atcgggcaac tccgtgatgc tgtcggacgc catcccggcc tgccgggctc cttcggccaa
      601 ctctcgtccc aatacaccac aggcgagcag atgtccgatt ccttgcgcgg ccagaaacgc
      661 cccgacctcc cggtgcatcc gcttgctgtc cgtgcccagc tccagcatgt cgcccagcgc
      721 cgcgatcgac cgttttcccc gaccgagctc cgccagcagt tgaacggccg ctttcatcga
      781 ggccggattg gcgttgtagc aatcgttgac cacctgcacc ccatgggaaa agctgatctg
      841 cgatcgcatc gcggccggcc gaaatttggc caaaccttcg gcgatggccg ttcccgataa
      901 tcccaacgca tacccgaccg cagccgcggc gagggcattg ctcacattgt gttgcccctg
      961 cgtccggatg cgaatttcag tctgacgact ctttcccggc accaccaggc cgaacaccgt
     1021 tccacccttt tcatcgaccc gaatattggc ggcgcggatt gctgccttgg gagacacccc
     1081 gaacgccacg acccggcact gcgcccgggc cgcgagataa tcaaaatatt catcatcggc
     1141 gttcaacacc accgccccgt cctgcggcaa atggtcgagc aactcggcct tggcctgcgc
     1201 agacccttcc atactgccga aaaactccaa atggtccggg ccgatgttgg taatcacgcc
     1261 aatcgtcggt ctcgcgattt cgcacagccg ggtggtctgc ccctgctgat ccacccccat
     1321 ttcaatgacg gcggcctgat ggcgcggcgc caactgaaac agagtctgcg ggactccgat
     1381 ccgattattg aggttgccct cggtcttgag cgttttccag cgctgtgcaa ccacctgggc
     1441 gaccatttcc ttcgtcgtcg tcttgccgtt gctccccgtg atggcaatga ccggaatggg
     1501 aaaccggttt cgatagtgcg tggccagccg ttgataggcc tcgagcgtat ctctcacccc
     1561 gagcagcatg ggcacgccgg tacgtttcgg cgccggcggc aggcgatagt cctcttgcac
     1621 gatggcacag accgccccct gggccaacac cttcggcacg aaggtatgcg cgtcaaaccg
     1681 ctcgccctgg aaggcgatga agagatcgcc tttgcgcacc aatcgcgaat cggtcaccac
     1741 acgacgaatg cgttgtttca agtcctgagg cgtgacgccg gccggcggtc tgacactcaa
     1801 gacctcacag atttcctcga ccgtaaacag cgccatcaca ccgcgattcc catgctcccg
     1861 cacgcgttcc ctcgctcagg ctccggatcg gagcgcgccg atcgtgtcgc gcgccacttc
     1921 ccgatcgtcg aagtgatgtt tcgtggttcc cacgatctga taatcttcgt gacctttccc
     1981 tgcgatcagc accatgtccc ccggcttcgc ctcacgaatg gccgcctcga tggccccccg
     2041 acggtctgca accatgcgat accgaacatg accccggtcc gccagcgcct ccttgacccc
     2101 gacctcgacc tcgcgcagga tcgcagcagg atcttcggta cgcgggttat ccgatgtcag
     2161 aatcaccaca tcactatact gcacggctgc acgtcccatt ttggggcgtt tggtccggtc
     2221 acggtccccg ccacacccaa agacagtgat gatgcgcccc gtgcgaagcg cctgggctgc
     2281 ggtcaacaac cgaaccagcg cgtcttcggt atgcgcatag tccaccacca cggaaaagtc
     2341 ctgaccggct tcgaccagtt cgaaccgccc cggcacgttc gtgaccgtct cgacagcgga
     2401 ccgtacctgc tccagcgtca gcccttcatg caacaccact ccgatggcag ccaggaggtt
     2461 cgacacatta tgctctccga ccagacggct cttgatcggg caggtcccga cgggactgcg
     2521 caacgtaaaa ctggtgccgg ccggcgagag cctcacgcct tcggcgtaaa tatcggcctg
     2581 ctggtgcagg ccgtaggtcc aaaccggaac ggtacaggcc gatcgaattc gcgcgcccca
     2641 gggatcatcc gcattgatga tcgcccgctt ccgcggcttc accgcgcccg cccttcctaa
     2701 ctcgacgaag agtttcagct ttgcctggaa ataacgctcc atatcgagat gaaagtcgag
     2761 gtggtcctgc gtgagattcg taaagacagc gacgtcgaac tcggaaccgg cagtccggtc
     2821 caaggccaac gcatgagacg agacctccat caccaccgta tccaacctcg cctcgcgcat
     2881 tcgtgcaaat aatttctgca actccaacgc gcccggcgtc gtatgcgatg ctggaatcga
     2941 ttccttcccg acgagatagg cgaccgtccc gatgagtccg acccggcggc gagcggcttc
     3001 cagcatggtt ttgacgacat acgtcgtcgt ggtctttcca ttggtgcccg tgacaccgat
     3061 catccgcaac gatgatgacg gatctccgta gaatcggctg ccgagaatcc cgagtgctgc
     3121 gcgcgagtcc tgcacacgaa cgcaggaaat cgcaccgacg tcgccgcttc gctgcaccac
     3181 gacggccgcc gcccccgccg cgaccacagg gtcgagaaac tgatggccat cgacccgctc
     3241 tccctgcaca gccacgaaga gggacccggg tcccaccttc cgcgaatcgt cagtcagatc
     3301 ggcaatggtc acgcgctggt ctccgcttcg ttccagcact ccgagacgcc catgaatcgg
     3361 accgatcaat tcatcgagtg tcatatcagg actcaaggcc ggccatggcc aacttgaccg
     3421 gctcatccac tgccacgccc agatagttga gtacctgctc accgacccga cggaataccg
     3481 gcgcggccac gacgccaccc cagccttcac cgcgcggctc gtcgatcacg acgatcatcg
     3541 ccagtcgagg atcttccgcc ggcacatacc cgagaaacga cccgaccagc agcgtggacg
     3601 aataggcgcc ggtgcgggga tcaacctttt gagccgtacc ggtcttgccg gccacccgga
     3661 agcccgggat cgccgctttg ccgccggtgc cgttcgtgac cactccctcc atcagcgtcg
     3721 tcagcgtcct cgccgtatcg gcagaaatga ctcgccgctt cgcttgcggc atggtttgcg
     3781 ccaccaattg tcccttggcg ttacggatct cggagaccac gaagggcttc atcaacacgc
     3841 ccccgttcgc aatggctgag acggccgtga ccatctgcag cggcgtcacg ccgacctctt
     3901 gccccatcga aatcgaagcc agagaacgct tgccccactg gcgcggtccc cgcagcaacc
     3961 ccacggcttc cccgggcaaa tcgatgccgg ttttgtcgcc gaacccgaag tctttgagat
     4021 aatcgaatac ccgccactca cccaaggcca tgccgacctt cgccgcgccg atattgctcg
     4081 acttctgaat catctgcgcg aaggtcatcc agccggtttt ttcgtgatcg tgaatcacgg
     4141 tgttggcgat cgagagctgc ccgttctctc catagatcat gcttccgggc gtcatcacct
     4201 tctcttcgag cgctgccgcc gcaatcaccg tcttcatggt ggacccgggc tcataggtat
     4261 ccgtcaaggc gcggttgcgc catcgatccg gagccaacgc cccgaccgtg ttgggatcga
     4321 atcgtggact gaccgccatg gccaacacgg ccccggtttt cgggtccatg acaatgatcg
     4381 tgccggactt ggcattcgat cgactcacgg cctcatccag ctctttctca gcgatgtact
     4441 gaatcacttc gtcgacggtg agggtcagac tgtgtccggc tgccgccccc tcctcattga
     4501 gtcccttcgg gaatacggcg cgtcccaacg catcacgttg cagcaccacc gcccgctttt
     4561 caccacgcag atattgctcg taacgcagct ccacgccctc caacccacga tcgtccatgc
     4621 cggcaaagcc cagcacgtgc gacagcagcg gccccttggg atagaagcgg cgcccttcca
     4681 tcacaacccc gactccatcg agagcaagtc gttcaagccg ccgcccctgc tccggctcaa
     4741 gctttcgcgc cagccacaca aaatgctttt cctgcttcag cttcttttcg agttccgtcg
     4801 ccttgacgtg caagatcggc gagagattgc gcgccgtcgc accgggattc ccgagggaag
     4861 ccggaactcc gaatacggac ggcacgtcca tgttcatcgc cagcaccttg ctgtttcgat
     4921 cgtagatcgt cccccgcgcg ccttccagcg tgacattttt ctgatgctgc cgatcggcct
     4981 tcacggtcag ctccgccgac tgcatcacct ggagattcac gagacggaca atgaccagca
     5041 tgaacgcgac caccagcccg caggccacca caatacggcg actgcgaaaa ggggtcgaag
     5101 ccactatcgc gctctccgcg ttacgatgtt tttggcgatc cgcacctcac cctccgcaac
     5161 gaccggattg gccggcgctt ccggctcgat gttgatgacg acgacctggc ccttctccgg
     5221 ctgcatcatc cccaacttgt cgctggccag acgcgcaatc cgctcaggtg ccgtgaggcc
     5281 ggagat
//
LOCUS       DAORRQ010000138         5163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_531_length_5163_cov_76.651527, whole genome shotgun sequence.
ACCESSION   DAORRQ010000138 DAORRQ010000000
VERSION     DAORRQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_531_length_5163_cov_76.651527"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PK224_20490"
     CDS             complement(<1..1377)
                     /locus_tag="PK224_20490"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRB18185.1"
                     /translation="MPAQAVSKGILSIGSWSVVKMATSALVLPILARMLGIEGYGQYA
                     YYMALLLLASQFANVGMMQTMTKRIAERPEDVAWCRAVAHAGALINAVGLLSVSVVVG
                     LVVGTTAPAGTAALPIALVIVGVLMFDQVWFYARGVLHGLRHEARAAIPGMIGVVSAG
                     LLGVLAATAGLGVTGVFAGLLTANLFVAMACLRAVLKVLSGSECRESGRTVSSVTGPL
                     LRFGLPAMLFSVLNMALCSLDVILVRHLAGEAQAGLYAAAIQWSQFVWFIPIAVEGVM
                     LQATARLWAEQRVDEVSQLVGRLVRYVVVGTTFLLLLVAVLGEQIVTVYFGPRFADAA
                     LGLRLLVPGALCYAVARVLWPVIQAGGEGGHLVRLIGMIVLVDVGLCGSLIPLWGAAG
                     AAMATSISFALVAVGYVWMLHRRRVQVFEQVHLFRWLLLSTGTALAIALVAAWVTPPL
                     LSILVGAVV"
     gene            complement(1440..2549)
                     /locus_tag="PK224_20495"
     CDS             complement(1440..2549)
                     /locus_tag="PK224_20495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB18186.1"
                     /translation="MKARPHVCILTSQYFDWGIYGGFGSMSRKLAESLVRSGHRVSVI
                     VPGRQGQQPSETIGGVEIRSFSPRNVVAACRLIRESTADIFHSQDPTVLTYLAQRLHP
                     RRAHLVTSRDPRELSDWWVEFRYATPMRRLLTPLNYFTESGLLVRRAVRRADAVFCPA
                     HFLKEKVKRLYGLSVLPTLLPNLIDVPASLPQKSARPTITFVARWDKRKRPWLFLELA
                     AQFPKYRFVAVGQGSASAESGFDAQLRRRFRDVPNLEMPGLINRFREPERMQRILSDT
                     WIFVSTAVREGLPLTFLEAAAYGCPIISRVDPDEFASRFGKQVTDDDYASAIRHLLAD
                     NPLEKGRAAYDYVRETYETSKALAAHQEQYERFAA"
     gene            complement(2546..3757)
                     /locus_tag="PK224_20500"
     CDS             complement(2546..3757)
                     /locus_tag="PK224_20500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB18187.1"
                     /translation="MFDNEFPPLGGGTGVVNYHLLQEMASHRDVVVDLVTSSRTKARY
                     ETEQFAERITIYKVPVDNRNIHHATNRELMRYSWRGLRMAQRLLRAHRYDVSFAFAGV
                     PAGAISYLLRLTHGVPYVLSLQGPDVPGFEARYAYLYPMLKPLIKRIWRHAEAVTAIS
                     AEQEALAHRTLPGLPLVTIPNGVDTTLFTPIHLESKRPFTIVCVARLIERKGQHHLLE
                     AFAQLRATCEQPLRLLFVGTGDAETALRESADRLQVADSVTFKGFVARDRMPAVYREA
                     DVFVLPSEQEGMSIALLEAMASGLPVIVTDTGGTAELVTKGENGEVVPWADVPALVRA
                     LRELLPADDLRRRMGAENRRRALRFGWPALATRYLELCARVMAPATAARPAAGRSIAD
                     AGGGWQREPRA"
     gene            complement(3775..4938)
                     /locus_tag="PK224_20505"
     CDS             complement(3775..4938)
                     /locus_tag="PK224_20505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB18188.1"
                     /translation="MQPPYKRAPPAPPAVQERKTVTSADRVGDRMLPPLEDPAEALRF
                     CRVLASALERASDGERHALTPANFAAMMGQAEPSTGRPELSVIIPVFNEAENLPTLHG
                     RLTRALVNLGMEYEIVLVDDGSQDQSPDILRRMEAEDQRIVIVEFARNFGHQVAISAG
                     LEQSRGQVVCIMDADLQDPPEVLHTFLAKWREGWEVVYAIRTERKEWWGKRLAYAGFY
                     RLLQRVANIDIPLDAGDFCVMDRRVVDLLVRMPERNRFVRGIRSWVGFKQIGVPYERH
                     ARHAGAPKYTFRKLLYLALDGLISFSHMPLRIITLLGFTVSFLSFLVALFYLVKKFTL
                     GTGVPGFTTLVVSIFFLAGIQLVTIGVIGEYIGRISDEVKRRPLYVARRVTRR"
BASE COUNT         1158 a         1753 c         1417 g          835 t
ORIGIN      
        1 caccaccgcc ccgacaagaa ttgagagcag cggaggcgtc acccatgctg caaccagagc
       61 gatagccagg gccgtgcccg tcgacaacag cagccaccta aacagatgaa cctgctcaaa
      121 gacctgaact cgccgccgat gcagcatcca gacatatccc actgccacca aggcaaacga
      181 gatggacgtg gccatcgccg caccggcagc gccccagagc ggaatcaagc tgccacagag
      241 ccccacatcc accagcacaa tcatcccgat gaggcgaacc aaatgtcccc cctccccgcc
      301 cgcttgaatg accggccaca acacacgcgc caccgcgtaa cacaaggccc ccggaaccaa
      361 caggcgcaat cccagggcag catcggcaaa acgagggcca aagtagactg tcacgatctg
      421 ttcgcccagg actgcgacca acaggagtag gaacgtcgtc cccaccacca cgtatcgcac
      481 cagccggccg accaactggc tcacctcatc cacccgctgt tccgcccaca ggcgcgccgt
      541 cgcctgcaac atgacgcctt cgaccgcaat cggaatgaac cagacgaatt gcgaccattg
      601 aatcgccgcc gcgtagagac ctgcctgcgc ctcgccggca aggtgccgaa ccagaatcac
      661 atccaacgag cagagggcca tattcagcac cgaaaacagc atggccggca gcccgaagcg
      721 caacaaaggt ccggtcactg aggagacagt ccgaccagac tcccggcatt cgctcccgct
      781 caataccttc agtaccgccc tgagacaggc catggccaca aagagattag ccgtcaataa
      841 cccggcaaac acgccggtca ctcccaaacc ggccgtggcc gccagcacgc ccaacaggcc
      901 tgccgatacc acgccgatca tgcctgggat cgccgcacgc gcctcatgtc gaagaccgtg
      961 gagtacaccg cgcgcataaa accacacctg gtcgaacatc aacacgccga cgatcaccaa
     1021 cgcgataggc agggccgccg tcccggccgg agcagtcgtc ccgaccacca ggcccaccac
     1081 gacactcacc gacagcaggc ctaccgcatt gatcaacgca cccgcgtgcg ccacggcccg
     1141 gcaccaggcc acgtcctccg gtcgctccgc gatacgtttc gtcatggtct gcatcatgcc
     1201 gacattggcg aattgagagg cgagcaacag gagggccatg taataggcat actgtccgta
     1261 accttcgatg ccgagcatgc gggcgaggat cggcaacacc aaggccgagg tggccatttt
     1321 caccaccgac caactgccga tggataggat acctttagaa accgcttgcg ccggcatgaa
     1381 tcttgtttcc gatcgaccgc agaggcctca tggaggagac gagacgaacg cctgaacgat
     1441 caggccgcaa atcgctcgta ctgttcttga tgcgccgcca gggccttgga agtttcgtag
     1501 gtctctcgca catagtcgta ggcggcccgc cccttctcca gcgggttatc ggcaagcaag
     1561 tgtcgaatgg ccgacgcata gtcgtcgtct gtcacctgct tgccgaaccg gctggcgaac
     1621 tcgtccgggt cgacccggct gatgatcgga cacccatagg ccgccgcttc cagaaacgtc
     1681 aggggaaggc cctcacgcac cgccgtgctg acaaaaatcc acgtgtccga cagaatccgc
     1741 tgcatccgct ccggctcgcg aaaacgattg atcagccccg gcatctccag gttcggcaca
     1801 tcccgaaatc tccggcgcaa ttgagcatcg aaaccggatt cggctgaggc actcccctgc
     1861 cccaccgcca caaaccggta cttcgggaac tgcgcggcca attccaggaa cagccagggc
     1921 cgtttgcgct tatcccagcg cgccacaaag gtgatcgtag ggcgcgcgct tttctgcggc
     1981 aacgacgcgg ggacgtcgat aaggttgggc aacaacgtcg gcagaaccga caggccatac
     2041 agccgcttca ccttctcctt caaaaaatgg gccggacaga acactgcatc cgctcgccgc
     2101 acggcccgcc gcaccagcaa cccggactcg gtgaagtagt tgagaggcgt caacaggcgg
     2161 cgcatcgggg tcgcataacg gaattcaacc caccagtcgc tcaactcgcg cgggtcgcgg
     2221 ctcgttacca ggtgggcgcg acgtgggtgg agacgttgcg ccagataggt caggacggtc
     2281 ggatcctgcg aatggaaaat atcggcggtg gattcacgaa tcaaacggca ggccgcaacg
     2341 acgttccgtg gtgaaaaact ccggatctcc accccaccga tcgtttcact cggctgttgt
     2401 ccctgtcgcc cgggaacaat gacactgacc cgatggccgg accgcacgag actctcggcc
     2461 aatttcctgg acatgctccc gaatccgccg tagatccccc agtcgaaata ctggctggtc
     2521 agaatgcaca catgcggccg cgccttcatg cccgtggctc ccgctgccac ccgccaccgg
     2581 catccgcaat ggaacgacca gctgccggcc gggctgccgt cgcaggtgcc atcaccctcg
     2641 cgcacaattc gagataccgg gtcgcgagcg cgggccaccc gaatcggagc gcccgccggc
     2701 gattctccgc acccatccgt cgtcgcagat cgtccgccgg cagcaattcc cgcaaggcgc
     2761 gcaccagcgc cggcacatcg gcccagggca cgacttcgcc gttctccccc ttcgtcacta
     2821 actccgcggt tcctcccgtg tcggtcacaa tgaccggcaa cccggacgcc atcgcctcca
     2881 acaacgcaat cgacatgccc tcctgctcgg acggcaacac gaacacatcc gcctcccggt
     2941 acacagccgg catccggtcc cgcgcaacga atcccttgaa ggtcacagag tctgccactt
     3001 ggagacggtc cgccgattcc cgtaaggcgg tctcggcatc gccggtaccc acgaatagca
     3061 gccgtagcgg ctgctcgcag gtggcccgca attgcgcgaa cgcttccaac agatgatgct
     3121 gtcctttgcg ctcgatcaaa cgcgcgacgc agacaatcgt gaatggccgc ttggattcca
     3181 ggtggatggg agtgaacaga gtcgtatcga ccccattggg aatcgtcacc agcggcaggc
     3241 ccggcagcgt gcgatgcgct aaggcttcct gctccgcact aatggccgtc acggcttccg
     3301 catgccgcca gatccgtttg atcaacggct tcagcatcgg ataaaggtac gcatagcgcg
     3361 cctcgaagcc gggcacgtcg ggcccttgaa gcgacagcac gtacggcaca ccgtgagtca
     3421 agcgcagcag ataactgatc gcccccgccg gcactccggc aaaggcaaag ctaacatcgt
     3481 accgatgggc gcgcagcaga cgctgcgcca tgcgcagacc gcgccagctg taccgcatca
     3541 actcgcgatt ggtcgcatgg tggatgttcc gattgtcgac gggaacttta tagatcgtga
     3601 tccgctccgc aaactgctcg gtctcatacc tcgccttggt ccgtgaagaa gtcaccaggt
     3661 ccacaaccac atcccgatgc gacgccatct cctgcaacag gtgatagttc accacgccgg
     3721 tgccgccgcc cagcggagga aactcattat cgaacatgag aatgcgaatg gcggctatct
     3781 cctcgtcacc cggcgggcca cgtagagagg gcgccgtttg acctcgtcgg aaatccggcc
     3841 gatgtactcg ccgatgaccc cgatcgtcac cagttgaatg cctgcgagaa agaagatgga
     3901 gacgaccagt gtggtaaagc cgggcacgcc ggtgcctagc gtgaacttct tgactagata
     3961 gaacagcgct acaaggaacg acaggaacga cacggtgaac cccaggaggg taatgatgcg
     4021 tagcggcata tggctgaagg agatcagccc gtccagcgcg agatagagga gcttcctgaa
     4081 cgtatacttg ggcgcccccg catggcgggc gtggcgttca tagggcacgc cgatttgttt
     4141 gaaccccacc caactacgaa tccccctgac aaaacgattg cgctccggca tgcgcaccag
     4201 gaggtccacc acccgccggt ccatgacaca aaaatctccg gcgtccaacg ggatgtcgat
     4261 attggccacc cgctgcagca ggcgataaaa cccggcatag gccaatcgct tcccccacca
     4321 ctctttacgc tcggtccgga tggcatagac gacctcccag ccctcccgcc acttggccag
     4381 aaaggtgtgc aacacctcag ggggatcctg caggtcggcg tccataatgc agacgacttg
     4441 tccgcggctc tgctcaagcc cggcactgat cgccacctga tgcccgaaat tccgcgcaaa
     4501 ctccacgatg acgatccgtt gatcctcggc ctccatcctt cgcaagatgt ccggactctg
     4561 gtcctgactg ccgtcgtcca ccagaacgat ttcatactcc attcccagat tcaccaacgc
     4621 cctggtgagg cggccatgca acgtggggag gttctccgcc tcattgaaca ccggaatgat
     4681 cacggacagc tcaggtcgcc cggtcgacgg ctccgcctgt cccatcatgg ccgcgaagtt
     4741 tgccggtgtg agggcatgtc tctcgccgtc ggaggcacgt tcaagcgccg aagccaagac
     4801 gcgacaaaat cgcaaggcct cggccgggtc ctccagaggc ggcagcattc ggtcgcctac
     4861 ccgatccgcg ctggtcacag tcttacgctc ctgcacagcc ggtggggctg gaggagcgcg
     4921 cttatatggt ggttgcatgg ttttccctac aatgtcaggc cttcggtctc ccgacagccg
     4981 cgctgtcggt cggcacaagg aatctccaac cacataagtg tagcaagaga atcgttttca
     5041 tccagttcac gctataaagc gcaaaaaatg gtttggatag gcgacaaaca tcgtcccgtg
     5101 acgaggcagg tgtaggaaaa aggacgggta tttttgtgtg aaatggacga cggccggaat
     5161 aca
//
LOCUS       DAORRQ010000139         5002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_540_length_5002_cov_433.636143, whole genome shotgun sequence.
ACCESSION   DAORRQ010000139 DAORRQ010000000
VERSION     DAORRQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_540_length_5002_cov_433.636143"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..2934)
                     /locus_tag="PK224_20510"
     rRNA            complement(42..2934)
                     /locus_tag="PK224_20510"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     assembly_gap    3115..3214
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3257..4799)
                     /locus_tag="PK224_20515"
     rRNA            complement(3257..4799)
                     /locus_tag="PK224_20515"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1067 a         1472 c         1042 g         1321 t
ORIGIN      
        1 tattcgtcta agtgctactt agcaaaaccc atctgggttg tatggttaag cctctcgagt
       61 cattagtatc agttagctca acgcctcaca acgcttacac acctgaccta tcaacgtcct
      121 agtctcgaac ggctctttag tggacttata gtccaaggga tgactcatct tggggctcgc
      181 ttcccgctta gatgctttca gcggttatcg attccgaaca tagctaccgg gcaatgccat
      241 tggcatgaca acccgaacac cagcggttcg ttcactccgg tcctctcgta ctaggagcaa
      301 ctcccctcaa tcatccaacg cccacggcag atagggaccg aactgtctca cgacgttctg
      361 aacccagctc gcgtaccact ttaaatggcg aacagccata cccttgggac cgacttcagc
      421 cccaggatgt gatgagccga catcgaggtg ccaaacaccg ccgtcgatat gaactcttgg
      481 gcggtatcag cctgttatcc ccggagtacc ttttatccgt tgagcgatgg cccttccatt
      541 cagaaccacc ggatcactat gacctacttt cgtacctgct cgacgtgtct gtctcgcagt
      601 taagctggct tatgccattg cactaaccgt acgatgtccg accgtactta gccaaccttc
      661 gtgctcctcc gttactcttt gggaggagac cgccccagtc aaactaccca ccaggcactg
      721 tccttacccc agataatggg gccaagttag aacatcaaca ctacaagggt ggtatttcaa
      781 ggacgactcc atcagaacta gcgttccaac ttcatagtct cccacctatc ctacacatgt
      841 agggtcaatg ttcagtgcca agctatagta aaggttcacg gggtctttcc gtctagccgc
      901 gggtatacgg catcttcacc gcaatttcaa cttcactgag tctcggctgg agacagcgtg
      961 gccatcatta cgccattcgt gcaggtcgga acttacccga caaggaattt cgctacctta
     1021 ggaccgttat agttacggcc gccgtttacc ggggcttcga tcatgagctt ctcttgcgat
     1081 aacccaatca attaaccttc cggcaccggg caggcgtcac accgtatact tcctcttgcg
     1141 agtttgcaca gtgctgtgtt tttgataaac agttgcagcc acctggtatc tgcgactccc
     1201 gtcagcttag agagcaagtc tcatcaccaa caggagcgta ccttctcccg aagttacggt
     1261 accattttgc ctagttcctt cagccgagtt ctctcaagcg ccttggtatt ctctacccga
     1321 ccacctgtgt cggtttgggg tacgattccc gctaacctga agcttagaag attttcctgg
     1381 aagcatggca tcaactactt catccccttg gggactcgtc atcagctctc agtgtatagt
     1441 gacccggatt tgcctaagtc acccacctac caccttaaac gcggactacc aacgccgcgc
     1501 tagcctagcc ttctccgtct ctccatcgca gttagcggaa gtacagaaat attaatctgt
     1561 ttcccatcga ttacgccttt cggcctcacc ttaggggtcg actcaccctg ccccgattaa
     1621 cgttggacag gaacccttgg tctttcggcg agggggtttt tcaccccctt tatcgttact
     1681 catgtcagca ttcgcacttc tgatacctcc agtgtgggtt accccttcac cttcaacggc
     1741 ttacagaacg ctcctctacc gcgcaactct aatgaattgc acccgtagct tcggtggtat
     1801 gtttagcccc gttacatctt ccgcgcaggc cgactcgact agtgagctat tacgctttct
     1861 ttaaatgatg gctgcttcta agccaacatc ctagctgtct aagccttccc acatcgtttc
     1921 ccacttaaca tacactttgg gaccttagct gacggtctgg gttgtttccc ttttgacgac
     1981 ggacgttagc acccgccgtc tgtctcccga gtagtactca ttggtattcg gagtttgcaa
     2041 agggttggta agtcgggatg accccctagc cttaacagtg ctctaccccc aatggtattc
     2101 gctcgaggcg ctacctaaat agctttcgag gagaaccaga tatctcccgg tttgattggc
     2161 ctttcacccc cagccacaag tcatccgcta atttttcaac attagtcggt tcggtcctcc
     2221 agttgatgtt actcaacctt caacctgccc atggctagat caccgggttt cgggtctacg
     2281 ccttgcaact aaacgcgcag ttaacactcg gtttccctac ggctccgcta ttcgcttaac
     2341 ctcgctacaa aacgtaagtc gctgacccat tatacaaaag gtacgcagtc acggtctcaa
     2401 gtaccgctcc cactgcttgt acgtatacgg tttcaggttc tatttcactc ccctcacagg
     2461 ggttcttttc gcctttccct cacggtactg gttcactatc ggtcagtcag gagtatttag
     2521 ccttggagga tggtcccccc atattcaaac aggatatcac gtgtcccgcc ttactcgttt
     2581 tcatcaaagg ttagttttcg tgtacggggc tatcaccctg tgccgctgga ctttccagac
     2641 cattccacta acacccctct gacttaaggg ctaatccccg ttcgctcgcc gctactaggg
     2701 gaatctcggt tgatttcttt tcctaagggt acttagatgt ttcagttccc ctcgtttgcc
     2761 tcattaagct atgtattcac ttaatgatga ccgcttatgc ggccgggttc ccccattcgg
     2821 acatcgttag ctcaaatgct tgttactagc tcgccaacgc ttttcgcaag ttactacgtc
     2881 cttcatcgcc tctgactgcc aaggcatcca ccgtatacgc ttagtcgctt aaccatacaa
     2941 cccaaatagg tttcacttgc gtgagccact tgcgctgttg cgaccagctg gttttacttg
     3001 tctcatcttc gaccaagaag atggactcgc cttagacttg aatattcaag acacttaaca
     3061 agtgttttaa gaactcaatt tttttcgtat taacacaacg acagacatca ttgtnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatctgt gtgaacactc aacaaacatc
     3241 gagttagtcg tataggtaag gaggtgatcc agccccaggt tcccctaggg ctaccttgtt
     3301 acgacttcac cccagtcatg aaccacaaag tggtgagcgc ccccccgaag gttaagctac
     3361 ccacttcttt tgcagcccac tcccatggtg tgacgggcgg tgtgtacaag gcccgggaac
     3421 gtattcaccg tggcattctg atccacgatt actagcgatt ccgacttcat ggagtcgagt
     3481 tgcagactcc aatccggact acgacgagct ttgtgagatt agctccacct cgcggctttg
     3541 caaccctctg tactcgccat tgtagcacgt gtgtagccct actcgtaagg gccatgatga
     3601 cttgacgtcg tccccacctt cctccggttt atcaccggca gtctccctag agttcccacc
     3661 attacgtgct ggcaaataag gataggggtt gcgctcgttg cgggacttaa cccaacattt
     3721 cacaacacga gctgacgaca gccatgcagc acctgtctca cagttcccga aggcacaacc
     3781 gcatctctgc agtcttctgt ggatgtcaag agtaggtaag gttcttcgcg ttgcatcgaa
     3841 ttaaaccaca tgctccaccg cttgtgcggg cccccgtcaa ttcatttgag ttttaacctt
     3901 gcggccgtac tccccaggcg gtctacttaa tgcgttagct tgagagccca gtgttcaaga
     3961 caccaaactc cgagtagaca tcgtttacgg cgtggactac cagggtatct aatcctgttt
     4021 gctccccacg ctttcgtgca tgagcgtcag tctttgtcca gggggccgcc ttcgccaccg
     4081 gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc cccctctaca
     4141 agactctagt tcgccagttc gaaatgctat tcctaggttg agcccagggc tttcacatct
     4201 cgcttaacaa accgcctgcg cacgctttac gcccagtaat tccgattaac gctcggaccc
     4261 tccgtattac cgcggctgct ggcacggagt tagccggtcc ttcttctgta ggtaacgtca
     4321 cagatgagcc gtattaagac tcaccctttc ctccctactg aaagtgcttt acaacccgaa
     4381 ggccttcttc acacacgcgg catggctgca tcagggtttc ccccattgtg caatattccc
     4441 cactgctgcc tcccgtagga gtctgggccg tgtctcagtc ccagtgtggc tgatcatcct
     4501 ctcagaacag ctagggatcg tcgccttggt gagccattac ctcaccaact agctaatccc
     4561 acctaggttc atccaatcgc gagaggcccg aaggtccccc tctttccccc gtagggcgta
     4621 tgcggtatta gcagtcgttt ccaactgtta tccccctcga ctgggcagat ccctaggcat
     4681 tactcacccg tccgccgctc gccacctcag gagtaaactc ccttgtgctg ccgctcgact
     4741 tgcatgtgtt aggcctgccg ccagcgttca atctgagcca tgatcaaact cttcaattaa
     4801 aagttttgtt gcttccgtct tgcgacttca gcggctcaat gaattctgat tgttgtttca
     4861 acatttccta gttaaaagaa gtgccaaaac aaactgtaca tattgctatg aacactcatt
     4921 cattgattta attctttgat tactcgccaa acggcagagt aatttcgatt aactcaacac
     4981 ctgtgagtgt ccacacagat tt
//
LOCUS       DAORRQ010000140         4990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_541_length_4990_cov_45.266464, whole genome shotgun sequence.
ACCESSION   DAORRQ010000140 DAORRQ010000000
VERSION     DAORRQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_541_length_4990_cov_45.266464"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK224_20520"
     CDS             <1..714
                     /locus_tag="PK224_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRB18189.1"
                     /translation="AYCYSVTPNLTPYVMLNFTGEARDIATMAHELGHAVHAMMAEQH
                     NVFTFHSTLPLAETASVFGERILSDALMATETNRSVKQSLLVNQLDDIYATVMRQAYF
                     VAFERTAHDMVAQGATTTDLAHTYMTLLRQQFGKSLRVPQEFQWEWLTIPHLYASPFY
                     CYAYSFGNLLVLALYRMYQEQGSGFVPKYLELLAAGGSKAPQAILAEVGVDMNSPAFW
                     QSGFDAISGMVDQLEQTMN"
     gene            complement(984..1229)
                     /locus_tag="PK224_20525"
     CDS             complement(984..1229)
                     /locus_tag="PK224_20525"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HRB18190.1"
                     /translation="MKKKTAPKKAASRPAKRMTSVTKETKAPGPVQDIQVRIAARAHE
                     LYEQRGCLDGYHLHDWLEAEREILGHPVVMDKSSERI"
     gene            complement(1406..1846)
                     /locus_tag="PK224_20530"
     CDS             complement(1406..1846)
                     /locus_tag="PK224_20530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB18191.1"
                     /translation="MEGYGKRVLIAEDEESVRRLLAVVLEEAGYTVHAVEDGMEALAE
                     MKKRRFDAVVADYRMPRLDGEQFLLLSRLMWPQTPTVLLSAEHTDLPVILKMQGACAL
                     VPKPFDSQGLLRTLQSAVEAACSCCSPSSIPREGTNNTLSVAPS"
     gene            complement(2142..2492)
                     /locus_tag="PK224_20535"
     CDS             complement(2142..2492)
                     /locus_tag="PK224_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18192.1"
                     /translation="MDPQVISPGDGAIDEQILGLLQHHRRLTFLTLAESLPLYTWQAL
                     FAALNRLRGQRHVDLLPLAADYEVVWQQGSERPSLMSEGPSRCDGDVAMDRTARRVRV
                     IEGGKGCPAVDVGV"
     gene            2882..3307
                     /locus_tag="PK224_20540"
     CDS             2882..3307
                     /locus_tag="PK224_20540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRB18193.1"
                     /translation="MSRVGIPADGFKTVGQIVGTNTIQFHTVQDGMAITIELLSAHVS
                     GGPVLDAEGRYVGFISEFDILKALEAGKDLNSLTATELMARDPIAVRTSTPIPDAIKL
                     MADNHLLNLPVEVNGEVTYSVTRHDLLRASIGLAVGIEE"
     gene            3335..3697
                     /locus_tag="PK224_20545"
     CDS             3335..3697
                     /locus_tag="PK224_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRB18194.1"
                     /translation="MIRIKRVYEIPSARDGIRILVDRIWPRGCTKEQARLDAWRKEVA
                     PSTALRTWFGHDPAKWMEFRTRYRKELSRPEQNRAIEDLARLAQSQTVTLLYGASDKE
                     HNQAVVLKECIDSAKNRV"
     gene            complement(4025..4681)
                     /locus_tag="PK224_20550"
     CDS             complement(4025..4681)
                     /locus_tag="PK224_20550"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HRB18195.1"
                     /translation="MNIRGQFIGFLIVAIVALAAVFFGHLFLFEQWRVQQERQLHRSK
                     ILGNVAHLQRLVLEVETNFRGYLLTEQQSFLEPIRLAENRLKTGLDQLTDLTASTPGL
                     QAGVSILSARLDEFVESKQRLLAVIEAEQQDLVRSYVRGGSGRALFLTIEKAIGDFEV
                     RIERELPSEPMTYDSWLERARWQLLLVESLGVMVCVFLTRAALLPNKTVLAPNPRPLL
                     "
BASE COUNT         1106 a         1570 c         1321 g          993 t
ORIGIN      
        1 gcctattgtt acagtgtgac ccccaacctg acaccctacg tcatgttgaa tttcaccggt
       61 gaagcgcggg acatcgccac catggcgcat gagttaggtc atgccgtcca cgcaatgatg
      121 gccgagcagc acaatgtctt caccttccac tccacgttgc cgctggcgga aacggcctcg
      181 gtcttcggcg aacggattct ctccgatgcc ctgatggcca ccgaaaccaa tcgatccgta
      241 aagcagagct tgctcgtcaa ccaactggac gacatctatg ccacggtcat gcggcaagcc
      301 tattttgtcg ccttcgaacg aaccgcccat gacatggtgg cccaaggcgc cacgacgact
      361 gacctggcac acacctacat gaccctgctc cggcagcaat tcggaaagtc cctgcgggtg
      421 ccgcaggaat tccaatggga atggctcaca atcccgcacc tctacgccag cccgttttat
      481 tgttatgcgt atagtttcgg gaacctgctc gtcctggcgc tctatcgcat gtatcaggaa
      541 caaggcagcg ggttcgtgcc gaaatatctg gagctactcg cagcaggcgg gtcgaaggcg
      601 ccgcaagcga tccttgcgga ggtgggagtc gacatgaact cccccgcctt ctggcaatcc
      661 ggattcgatg cgatttcagg aatggtcgat cagttggaac agacgatgaa ctaatcggac
      721 ggcgccaccc ttgacttcaa gggcggcgcg gactatccga acattggcag ccgattattc
      781 acgcatctag cccgccttat tcatgacggt tcgtcgcgaa attgcggagt cgcaaagcga
      841 gacgggcgcg cggagccggg agggaggaag gcatccttga acaggatgtc gatcgaccga
      901 gcggcgagcc cgcccgccga caggctggtc gtagctgcga atccgcgatt gcagcagaag
      961 cgctcatgaa taatgcgggc tagtcagatt cgctctgagg acttatccat caccaccggg
     1021 tgtcccagga tttcccgctc tgcttccagc cagtcatgca ggtggtaacc atccaaacaa
     1081 ccccgttgtt catacagttc atgcgcccgt gccgcaatcc gtacctggat atcctggacc
     1141 ggaccgggag ccttggtttc tttggtcact gacgtcatcc gtttcgccgg acgcgaggcg
     1201 gccttcttcg gcgccgtctt tttcttcatg cattctccat ctacacttgt atccagtcat
     1261 caggcccgcc tctcaccata cttttcgcga gagatctgtt cctacgatac catattcagg
     1321 tcggatctga tcaggcggca gaacgtgccc ctcaaccccg tctccctcct tcaagcacag
     1381 acaccttcct cgaggtgacc acccgctagg aaggcgctac tgagagagtg ttattcgtcc
     1441 cctcacgcgg gatcgaactc ggggagcaac aactacaagc cgcctctacg gcagactgca
     1501 acgtccgtaa cagcccctgc gaatcgaagg gtttcggcac gagagcacag gcgccttgca
     1561 ttttcaggat cacgggcaaa tccgtatgct cggcggacag gagcacggtc ggcgtctggg
     1621 gccacatcag gcgactcagc agcaggaact gctccccatc caggcggggc atgcggtaat
     1681 ccgcgaccac cgcatcgaaa cgccgcttct tcatttcggc cagggcttcc atgccatctt
     1741 ccaccgcatg cacggtatac ccggcttcct cgagcaccac ggccagcagg cgccgcacac
     1801 tctcctcgtc ctccgcaatc aacacacgtt ttccataccc ttccatggca cctcctccct
     1861 ccgactgcat tccaacgacg gaataccctc attccttccg tcacgaccat aatcccgtcc
     1921 gcccaaacgg catagctact ctggttccgg tttccataac cgacgaatcc atatgcctct
     1981 gcgttgcgga ccagggcgaa cccttcgatg aagttgttcc ggaaggcgtc gtgccaggcg
     2041 gattaacgga tcttccacat tgccgcaatt gaggcaacgc gtgaccgaca cccacgtatg
     2101 cgacactcct tcgctgggac taaacaggtt ttcggccacc atcagacccc gacatcgact
     2161 gcaggacatc ctttgcctcc ttcgatcact cgcacccgcc gtgcggtcct gtccatcgca
     2221 acatcgccat cacatcttga gggcccttcg gacatcaggg acgggcgttc gctcccctgc
     2281 tgccagacca cttcgtaatc tgcggccagc ggtagcaggt ccacatggcg ttggcctcga
     2341 agccggttca acgccgcaaa caatgcctgc caggtgtaca atggaaggga ttcggccagg
     2401 gtgaggaagg tcagccgccg gtggtgctgc agcaaaccga gaatctgctc gtcgatggcg
     2461 ccatcgcccg gagaaataac ctgagggtcc atcacgcacc tccattgtgg acagagcgga
     2521 ctactcgctt cacgtgtggt ccagcccatc cccggagacg ttgcaatatg cgatcctgct
     2581 gacggcacac atccagcgaa cggaatccgc cattcagcaa tggttacggc atgctcactc
     2641 cgcatccagc tgcgcttaac cgggacattc cgcactgtct gtcccgtgcg gaatcgctcg
     2701 cacccacagg atcatcgcct gcaacggcgc aggaattcag gaaatcgcaa cccatccgga
     2761 cgggcacttc gcttgctgag ctgatgaaca cgagttcgcg ggaacagcgc tggccgcagt
     2821 tcatcgacct gccacacatc tcgcaccctc gatggctgtt gccgcatcga aaggagacat
     2881 catgagtcgc gtaggaattc cagctgacgg tttcaaaacc gtcggacaaa tcgtcggaac
     2941 caatacgata caattccata ccgtacagga tggcatggcg atcaccatcg agttgctttc
     3001 cgcgcatgtg tccggcggac cggtgttgga tgcagagggc cgctatgtgg gattcatcag
     3061 tgaatttgat atcctcaagg cccttgaggc aggaaaggac ttgaacagtc tgacggcgac
     3121 agagctgatg gccagggatc ccattgcggt ccgtacatcg acaccgatac ccgacgcgat
     3181 caagctcatg gcagacaacc acttgctgaa cctccctgtc gaggtgaacg gagaggtgac
     3241 ctattcggtg accaggcacg acctgctccg tgcatcgatc ggtcttgccg taggcatcga
     3301 agagtagccg cgcgcggatt gttgctgtgc aatcatgatt cgaatcaaac gggtctacga
     3361 aataccaagc gcgcgcgatg ggattcggat tctcgtcgac cggatatggc ctagggggtg
     3421 cacgaaagag caggcacgtc tcgatgcatg gcggaaggaa gtggctccca gcacggcgct
     3481 ccgcacttgg ttcggccatg acccggcaaa gtggatggaa tttcggacac gttaccgcaa
     3541 ggaactgagc cgaccggaac agaaccgcgc gatcgaagat ctcgccaggc tcgcacagtc
     3601 acagaccgtc acactgctct atggggcatc agacaaggaa cataatcagg ccgtggtctt
     3661 gaaggaatgc atcgacagtg cgaaaaatcg cgtttagctc gtcaatcccc aggtgctcca
     3721 gagagcaacc gcggcaatcg ccaacatcac ccaggctaac tcccgatctc cgactttctt
     3781 gatcatccgt accatgggtt gactccttgt ttcgtagacg ttcactgtga tgaacgtctt
     3841 tagagcaagg tacatgccat tactcatgaa cggaattcca agggttttca gattctgcca
     3901 gcctcaaatc gagcgaattc gcacgctacg cactgtgcga atcgacagat ggagagggac
     3961 ttggggcgta gatcgcgaca ggtgacgaat cgatgggagg aacaactcga tagcccggat
     4021 agtctcacaa aagaggtcga gggttcggcg ccagcacggt cttattcgga agaagcgccg
     4081 ctctcgtcag gaagacacac accatgaccc ccagactttc caccaacaac agctgccagc
     4141 gtgctcgttc cagccacgag tcataggtca tgggctcact cggcagttcg cgctcaatgc
     4201 gcacttcaaa atctccgatg gctttttcga tggtcaggaa caacgcccga ccactccctc
     4261 cccgcacata ggagcggacg aggtcctgct gctccgcctc gatgactgcc agcaatctct
     4321 gtttgctctc cacgaattca tcaagccggg ccgaaaggat actcactccg gcctgcaaac
     4381 ccggagtgga ggcggtcaaa tcggtcaatt gatccagtcc tgttttcagt cgattctccg
     4441 ccagccgaat cggttcgaga aacgactgct gctccgtgag cagatagccg cgaaagtttg
     4501 tttccacctc caagaccaac cgttgcaaat gcgctacgtt gccgagaatt ttggaacggt
     4561 ggagctgccg ctcctgctgt acccgccatt gctcaaacag gaacaggtgg ccgaagaaca
     4621 ccgcggccag cgcaactatg gcgacgatga gaaaccctat gaactgtccc cggatattca
     4681 tcactctctg tcacgcattc caagcctcag gcaatcagcg gatccaaacg tgatggcgcc
     4741 ccttcagcct tcgcccggac ttcacccacg ctgcgtggca tcatcggcca gtaaccggcg
     4801 gggccagtcc ccgtgaagga tcgctccctc acgaagacta cccgccggcc ttccactcac
     4861 aggatcggcg gcacaggccc aagcgcaacg tcatcgttgc atccgggcgc gaccactcga
     4921 ccttacagcc agttgacgcg ctcggccggc cgaatgtaga tcggctcttc gacctgaata
     4981 cgcgccactt
//
LOCUS       DAORRQ010000141         4929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_545_length_4929_cov_10.679319, whole genome shotgun sequence.
ACCESSION   DAORRQ010000141 DAORRQ010000000
VERSION     DAORRQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_545_length_4929_cov_10.679319"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="PK224_20555"
     CDS             <1..1861
                     /locus_tag="PK224_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HRB18196.1"
                     /translation="TPVVLWAGWPFFQRGWASIVHRSPNMFTLIAIGTGTAYLYSVFA
                     TLFPALIPQSFHLESGAVPVYFEAAAVITVLVLLGQVLELRARSRTTGAIRALLGLAP
                     KTARRMREDGREEDVSLDQVQVGDRLRVRPGERVPVDGVILEGATAIDESMITGESLP
                     VEKTTGERVTGGTINGSGGLVMRADRVGADTLLAHIVQMVAEAQRSRAPIQRTADVVA
                     GYFVPIVVSVAIVTGLVWALIGPEPRLAHALLNAVAVLIIACPCALGLATPMSIMVGT
                     GRGAAAGVLFKKAEAMETIEKVDTLVFDKTGTLTEGKPKLRVVSAVAPWSEIDLLRLA
                     ASIERGSEHPVAGAIVSGAELRGITLESASGFTSKTGKGVMATVGTRRVAVGTLDLMR
                     EMQLDDETSLPALEADAELMRQTGQTVMFVSVDGRVAGLLGVADPIKSSTPEALRLLR
                     QEGIRLVMVTGDHAVTAQAVAKELGLDEVRAGVKPDEKSRIVQELQQQGHVVAMAGDG
                     VNDAPTLAQADVGIAMGTGTDVAMENAGVTLVKGDLRGIVRARRLSKATMRNIRQNLF
                     FAFVYNMIGVPVAAGLLYPFLGLLLSPMLASTAMTFSSLSVISNALRLRHADL"
     gene            2000..3433
                     /locus_tag="PK224_20560"
     CDS             2000..3433
                     /locus_tag="PK224_20560"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRB18197.1"
                     /translation="MSSRGSQTGLLALGIAGVLLGWWAESSASAADLLAPRLSKVSTA
                     GDLQAQVRATAAKVLPAVVSIASTVMVHDQAFTDEGLPFGMFKDVPPRRQYGQGSGVI
                     VSSDGYIITNNHVVADAVDVEVILADRRQFKGRVVATDPKTDVAVVKISATGLPAAAW
                     GDSSALAVGDFVLAIGNPLGLSRTVTFGIVSAVGRADVGVADVEDFIQTDAPINPGNS
                     GGALVNTNGELVGINTAIASPTGGSVGVGFAIPSNMARTAMQSLIKTGRVVRGFLGAS
                     TQDVTPLLAKTFHLPDVKGSIITDLQAKGSAERAGLKRGDVVVRFDGRDVMDSGHLRN
                     LMATAAIGSKHRIELLRDAKLMQTDLTVQEAPRERPKKTQTADAAGATAHPLSGVIVD
                     EVTPALARQMDLPANSGLVVTDIEDGSLAEVSGLQPGDLLLELNRQPIPNFSTFQRLA
                     EPLRSTDLALLLINRQGSLLYIPIQSE"
     gene            3607..3981
                     /locus_tag="PK224_20565"
     CDS             3607..3981
                     /locus_tag="PK224_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18198.1"
                     /translation="MWSISRVSCLLALWLCAACAPEPEAAPSYLVGVWETAAEGYTDQ
                     HMFIDEHRIGFGTSAVTADGYVITQIDESREGTKTLFVVSYQGEDQTKYQLAFFYEPA
                     AGGRIIYKNQDHLVWTRKVATT"
     gene            3978..4613
                     /locus_tag="PK224_20570"
     CDS             3978..4613
                     /locus_tag="PK224_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRB18199.1"
                     /translation="MSRPRFRRHFLWDTVQPRFLGLSFCYVVVVIAAVAGALFVPLML
                     ELNHLPLSSIEAQRVADQFELLHSRFWPVVFVVSLLLIVHGVFFSHRIAGPLYRFRRI
                     FQSVASGDLTVRTSIRKADYLHVEAQCLGEMVDALREKIGRIEAHHADIAPQLERLKA
                     AAACGALREVEQEADRLRATVEQLTRAMEPFQTTPNVIEARPVQLPSASGF"
     gene            4621..>4929
                     /locus_tag="PK224_20575"
     CDS             4621..>4929
                     /locus_tag="PK224_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRB18200.1"
                     /translation="MAHSTHCRRQQGFTIIELMIAMAIVGALAGLAIPNYLGFLDKAR
                     VARAIAEIRYIERVIDSYEAAYDTLPNSLADAGAGEMVDPWGNPYEYLNIAALTLPGN
                     G"
BASE COUNT          886 a         1398 c         1639 g         1006 t
ORIGIN      
        1 cacaccggtg gtcctctggg ccgggtggcc gttctttcag cgcggctggg cttcgatcgt
       61 ccatcgcagc ccgaacatgt tcacgctgat tgcaatcggg accggcacgg cctacctcta
      121 cagtgtgttc gccaccctct tccccgcgct gattccgcaa tcgttccatc tggaaagcgg
      181 agcggtgccg gtgtatttcg aagccgcggc ggtcattacg gtcctggtcc tgttggggca
      241 ggtattggaa ttgcgggcca ggagccgaac caccggcgcg atccgggctc tcctgggact
      301 ggcgcccaag accgcgcggc gcatgcgaga ggacggccgt gaagaagatg tgtcgctcga
      361 tcaggtgcag gtgggcgatc gcctgcgtgt gcgaccggga gaacgggtgc cggtcgatgg
      421 ggtgattctg gaaggagcga ccgcgatcga tgaatcgatg attaccggcg aatcgttgcc
      481 ggttgagaaa accaccgggg agcgggtgac gggcggcacg atcaacggtt ccggcggtct
      541 ggtgatgcgg gcggatcgcg tcggggcgga tacgctgttg gcgcatatcg ttcagatggt
      601 ggcggaagcg caacgcagcc gggctcccat tcaacggacc gccgatgtcg tggccggcta
      661 tttcgtgccc atcgtggtga gcgtggcgat cgtgactggc ctggtgtggg cgttgatcgg
      721 gccggaaccg cgcctggccc atgccctgtt gaatgcggtt gccgtgctga tcattgcctg
      781 cccctgcgcg ctgggtctcg caacgccgat gtccattatg gtggggaccg gtcgcggtgc
      841 tgcggccggt gtcctgttca agaaagctga agcgatggag accatcgaaa aggtcgatac
      901 gctggttttc gacaagaccg gcacactcac ggaaggtaaa cccaaactgc gcgtcgtgag
      961 cgccgtggcc ccctggtctg aaatcgacct gttgcgattg gcggcgtcta tcgagcgggg
     1021 gagtgaacat ccggtagccg gcgcgattgt gagcggagct gagttgcgag gcattacatt
     1081 ggagtcggca tctgggttca cgtcaaaaac ggggaagggc gtcatggcga cggtcgggac
     1141 caggcgggtg gcggtgggga cgctcgattt gatgcgtgag atgcagctcg acgacgaaac
     1201 ctccctgccc gcactcgaag ccgatgccga attgatgcgt cagaccggac aaaccgtgat
     1261 gttcgtctcc gtcgatggcc gggtggccgg cttgctcggg gtggccgacc cgatcaagag
     1321 ttcgaccccc gaggccttgc ggttgttgcg ccaggaaggc attcgtctgg tgatggtgac
     1381 cggcgatcat gcggtgacgg cgcaggcggt ggcgaaggaa ttaggcctgg atgaagtcag
     1441 ggccggcgtg aagccggatg aaaagagccg gatcgtccag gaacttcagc agcagggcca
     1501 tgtggtggcg atggcaggtg atggcgtcaa cgatgctccg acactggcgc aggcggatgt
     1561 cggcattgcc atgggaaccg gaaccgatgt ggcgatggag aatgccgggg tgacgctggt
     1621 gaagggcgat ttgcgcggga ttgtccgggc acgccgcctg agcaaggcca ccatgcgcaa
     1681 catccgccag aatctcttct tcgcctttgt gtacaatatg atcggggttc cggtcgccgc
     1741 cgggctgttg tatccgttcc tcggccttct cctcagtccg atgttggcca gcacggcgat
     1801 gacgttcagt tccctctcgg tgatttcgaa cgcgttacgc ctccggcatg ccgatttatg
     1861 agttctgagt gtgcaagcct ctgccgcgtg ataggctggg acctgcggtt tattttgacg
     1921 cgagacggca ccggagctca gtccaagcta ttgcgcgaca agctagtttt tgtgcctcct
     1981 gctagggatg tttcacacca tgtcgtcacg tggatcacaa acgggcctgt tggctctggg
     2041 aatcgcgggc gtcctgctgg gctggtgggc tgagagttca gcctcggctg ctgatttgct
     2101 tgcaccgcgt ctgtcaaagg tcagcacggc cggtgatttg caggcccagg tgcgggccac
     2161 cgcggccaag gtgctccccg ccgtcgtcag catcgcctcg acggtgatgg tgcacgatca
     2221 agccttcacc gatgaggggc tgcctttcgg catgttcaaa gacgtgccgc cgcgccgcca
     2281 atacgggcag ggctccgggg tcatcgtctc gtcggacggg tacatcatca ccaacaacca
     2341 cgtcgtagcc gatgcggtgg acgtggaagt cattctcgcg gaccgacggc agttcaaggg
     2401 ccgcgtggtg gcgaccgatc cgaaaaccga tgtcgcggtg gtaaaaatca gcgccaccgg
     2461 tcttcccgct gcggcctggg gtgactccag tgcgctggct gtcggtgact tcgtcctcgc
     2521 aatcggcaat cccctcggct tgagtcgcac cgtgaccttc ggcatcgtca gcgcggtcgg
     2581 ccgtgcggat gtgggagtgg ccgatgtgga agatttcatt cagaccgatg cgccgatcaa
     2641 tcccgggaac tccggtggag ctctggtgaa caccaacggc gagttggtcg ggatcaacac
     2701 ggctattgcc agtccgacgg gcggcagcgt cggcgtcgga tttgcgattc ccagcaacat
     2761 ggcgagaacc gccatgcaga gtcttatcaa gacgggccgg gtcgtccggg ggtttctggg
     2821 ggcctccacg caggatgtca cgcccttgct cgccaagacc ttccacctgc cggatgtgaa
     2881 gggctcgatc attaccgatc tgcaggcgaa ggggtccgcc gaacgggccg gattgaagcg
     2941 cggcgatgtc gtggtgcgtt tcgacgggcg cgatgtgatg gacagcggcc atttgcgtaa
     3001 tctgatggcc acggcggcca tcggcagcaa acaccggatc gagttgctcc gcgatgccaa
     3061 gttgatgcag acggacctca ccgtgcagga agcgccgcgc gagcggccca agaaaacgca
     3121 gacggccgac gccgccggtg ccaccgcgca tcccctgtcc ggcgtgatcg tcgatgaagt
     3181 cactccagcg ctggcgcgac agatggatct tcctgccaac agcggcctgg tggtcacgga
     3241 tatcgaagac ggcagcctcg ccgaggtgtc ggggcttcag cccggcgatc ttctgttgga
     3301 actcaatcga cagcccatcc ccaacttcag cacgtttcag cgactcgctg aaccgctccg
     3361 gtccaccgat ctcgcactgt tgctcatcaa ccgccagggc agtctgctct acatccccat
     3421 tcaaagcgaa taaccgggtt cttgtcctct ctcaagaatc gacctgcact cacggctgaa
     3481 tgtgccgccg ggtcggtgct gatttcctgc gagaattctt aaagaaattg agtttggacg
     3541 caggtacggc atactgagtc ggtgccggag agagactcag aagactccaa ctcaaagagg
     3601 tgtatcgtgt ggtcgatctc gcgggtgagt tgtctgctcg ccctctggct gtgcgcggcc
     3661 tgtgcgcctg agccggaggc ggcgccgtca tatctcgtcg gggtgtggga aacggcggcc
     3721 gaaggttaca cggaccagca tatgtttatc gatgagcacc ggatcggatt cggtaccagt
     3781 gccgtcacgg cggatgggta tgtgatcacg cagatcgatg aaagccgtga ggggaccaag
     3841 accctcttcg tcgtctccta tcagggtgaa gaccaaacta agtaccaact ggcctttttt
     3901 tatgagccgg ccgctggcgg caggattatc tataagaatc aggaccattt ggtctggacg
     3961 agaaaggtcg cgaccacatg agccggccac gattcagacg gcatttcctg tgggatacgg
     4021 tgcagccacg cttcctgggg ttgagcttct gctacgtggt ggtggtcatt gcggccgtgg
     4081 cgggggcgct cttcgtgccg ttgatgctgg aacttaatca cctgccgctt tcctccatcg
     4141 aagcccaacg agtggccgat cagttcgaat tgctgcacag ccggttttgg ccggtggtgt
     4201 tcgtcgtttc cctcctgctg atcgtgcatg gcgtgttttt ctcgcaccgt attgccggtc
     4261 cgctctatcg ctttcgccgg attttccagt ccgtcgcgag cggcgatctg accgtgcgca
     4321 cctcaattcg caaggccgat tatctccatg tggaagcgca atgtttgggt gaaatggtcg
     4381 acgcgttgcg ggagaagatc ggccggatcg aggcgcacca tgccgacatc gctccgcagt
     4441 tggaacgatt gaaagccgcc gcagcctgtg gggcgttgcg ggaagtggag caggaggccg
     4501 accgattacg tgcgaccgtc gaacagctca cgcgggcgat ggaaccgttt cagaccacgc
     4561 cgaatgtcat cgaggcccgg ccggtgcagt tgccctctgc gtcgggattt taggttgcgt
     4621 atggcgcaca gtacacactg ccgcaggcag cagggcttta ccatcatcga attgatgatc
     4681 gccatggcga tcgtcggggc gttggcgggt ctggcgattc ccaactatct gggatttctc
     4741 gataaggcgc gagtggcgcg agcgatcgcc gaaatacgct acatcgagcg ggtgatcgat
     4801 tcgtacgaag ccgcctatga tacccttccg aacagcttgg ccgatgcggg tgccggggag
     4861 atggtcgatc cctggggcaa tccctacgag tacctgaaca ttgcggccct aacgctcccg
     4921 ggaaatggg
//
LOCUS       DAORRQ010000142         4852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_553_length_4852_cov_12.258078, whole genome shotgun sequence.
ACCESSION   DAORRQ010000142 DAORRQ010000000
VERSION     DAORRQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_553_length_4852_cov_12.258078"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>230
                     /locus_tag="PK224_20580"
     CDS             34..>230
                     /locus_tag="PK224_20580"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRB18201.1"
                     /translation="MSAFPPAAQDVRERRTVLVTGGSGGIGRALCLAFAHAGYWVGVH
                     YFRQRPAAEQTLALLSQAGGQG"
     assembly_gap    231..240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <241..797
                     /locus_tag="PK224_20585"
     CDS             <241..797
                     /locus_tag="PK224_20585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB18202.1"
                     /translation="LYQADVRSSHEVQAMLDAIRAQRGRLDTLLCNAGIASKHLVVNC
                     PEEAWQQIIDTNLTGTYRCMTAAVNDMMPQGGGSIVVIGSFAGLQGTNGQAAYAASKA
                     GLIGLVKTAAREWGPLNIRVNMVCPGWQQTRIAGDSFPTGDRLDDHVLGRVSNLADVS
                     RTICQLAQLSDMSGQVWNLDSRIL"
     gene            950..1720
                     /gene="bioD"
                     /locus_tag="PK224_20590"
     CDS             950..1720
                     /gene="bioD"
                     /locus_tag="PK224_20590"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRB18203.1"
                     /translation="MGIATGVFVTGTDTGVGKTLVSSALVRRLVQTGRSVGVMKPVET
                     GVLPEQLSESDAGRLMAAAGVSDGIDEVSPYRFPAPLAPLAAASAQGSVIEMQEIMGR
                     YERLAARDSCVVVEGAGGLLVPMGKDWDMRDLIGRIGLPVVLVGRAGLGGINHALLTL
                     EGLRQRHCAVMAVVLNETALSTTPAQQEQCASTVALLRERAGVPVLGPLPYQPQLGTD
                     WSGAVEALAQSSPIRELANLVWANAVGMFGSPRRGPEP"
     gene            complement(1644..2258)
                     /gene="thiE"
                     /locus_tag="PK224_20595"
     CDS             complement(1644..2258)
                     /gene="thiE"
                     /locus_tag="PK224_20595"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRB18204.1"
                     /translation="MLDPTVRPDRPLPDLLREAAAHGVRLFQYRDKQATMRDAYAKAL
                     ALRQAATDAGALLIVNDRCDLAMAVNADGVHLGQDDLPISYARRLMGPGKIIGLSTHN
                     AAQVREAVTAQPDYVGFGPIFSTSTKADHDPVVGLEGLRAARALTTLPMFAIGGITAE
                     SVGEIMAAGADGVAVISAILKAPDLGAAIRTFQQHLPTPDSQAP"
     gene            complement(2490..3839)
                     /gene="accC"
                     /locus_tag="PK224_20600"
     CDS             complement(2490..3839)
                     /gene="accC"
                     /locus_tag="PK224_20600"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRB18205.1"
                     /translation="MFKKVLVANRGEIALRVIRACKELGIKTVAIHSEADAASLHVRA
                     ADEHVCVGPPEAALSYRNIPNVLSAAEITGADAIHPGYGFLSENAHFAEVCESIGVKF
                     IGPTSENIALMGDKSKAREVVAKKGLPVTPGSPGELRSEQDAQEAAKTIGFPVIIKAS
                     AGGGGRGMRVVNKPEELARAFQAAQAEAKSTFGHDGVYLERYFLEPRHIEVQIVADNR
                     GHVVHLGERDCSIQRRHQKLVEETPSPAIDEGLRREIGRVAVQAVKAIRYCNVGTVEF
                     LLDKDRNFFFMEVNTRIQVEHPITEMVTGIDLVKEQIRIASGMPLSFKQPDIKLNGHS
                     FECRINAEDPEKFTPCPGQITKYSAPGGFGVRVDSAMEPNAVVVPYYDSLIAKLITHG
                     RDRQEAMARMRRALDEFVIEGIKTTIPLHRKIFNDPDFQKGHVSTTFLDRFLAGQSS"
     gene            complement(4022..4597)
                     /gene="accB"
                     /locus_tag="PK224_20605"
     CDS             complement(4022..4597)
                     /gene="accB"
                     /locus_tag="PK224_20605"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRB18206.1"
                     /translation="MSSSGKKTGKGRGAGKPIILPSAFAPRSTAAETLLSPDQAAQIQ
                     QLADLLKRNHLTELEIERSGVRIRIRHEPVIRTTTAQTVETATNQSTSTGATPAAQTR
                     PPAETDGQVTITSPIVGTFYRSPSPDADPYVEEGDYVRKGQVLCIVEAMKLMNEIESE
                     ADGRITKILAESTKPVEYGQPLFLIDPNATP"
     gene            complement(4594..>4852)
                     /locus_tag="PK224_20610"
     CDS             complement(4594..>4852)
                     /locus_tag="PK224_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRB18207.1"
                     /translation="ADLLKENMIAKILIYEHRPITVLLPNFIELKVVDGEPGVRGDTA
                     SGGNKPVIVETGATIKVPLYLEIGETIRIDTRTREFVERVR"
BASE COUNT          835 a         1392 c         1558 g         1057 t
ORIGIN      
        1 ctcctgtgcc tcctctcata atcgcacgcc gtgatgtccg cattccctcc cgcagctcaa
       61 gatgttcgtg aacgccggac ggtactggtg accggtgggt ccggcggaat cggtcgtgcg
      121 ctctgtctgg ccttcgccca tgccgggtat tgggtgggag tgcattactt ccgccaacgg
      181 ccggcagccg aacaaaccct cgcgctgctc tcgcaggccg gtggtcaggg nnnnnnnnnn
      241 ccctctacca ggcggatgta cgatcgagcc atgaggtcca ggcgatgctg gatgcgatcc
      301 gggcgcagcg cggccggctc gacaccctgc tctgtaatgc cggcatcgcg tcgaagcatc
      361 tggtggtgaa ttgcccggaa gaagcatggc aacagatcat cgacacgaat ctgacaggca
      421 cctatcgctg catgaccgcc gcggtgaacg acatgatgcc gcagggcggt gggtccatcg
      481 tcgtcatcgg gtcctttgcc ggattgcagg gaacgaacgg ccaagccgcc tatgcagcgt
      541 cgaaggcggg gctgatcggc ttggtgaaga ccgcggcgcg ggaatgggga ccgcttaata
      601 ttcgcgtgaa tatggtctgt cccggctggc agcaaacgcg catagccggt gactcgtttc
      661 caaccggtga tcggctcgat gaccatgtgc tgggacgtgt ctcgaacctg gctgatgtga
      721 gcaggacgat ttgccaactc gcgcagctct ccgatatgtc ggggcaagtg tggaacctgg
      781 acagtcgcat tctctaacag gatgctgaaa acggcctcca gcttcgttct cggtggtcgt
      841 gaagcgtgaa tagtgaaacg tgaaatgtag gactagcgaa ggctattctt ctgggagctt
      901 gtgacgtttg acgtttcacg tctgatgaat gtcattcgat gggaagagaa tgggaatagc
      961 cacaggggtg tttgtcaccg gtacggatac cggcgttgga aagaccctcg tgtcctccgc
     1021 attggtccgg aggctggtcc agactggccg ctctgtcggg gtgatgaagc cggtcgaaac
     1081 cggggtcctt cccgagcaac tgagtgagag cgacgcgggg cgactcatgg ctgcggcggg
     1141 agtatccgat gggatcgatg aggtgtctcc ctaccggttc cccgcgccgc tcgcccccct
     1201 tgccgccgca tccgcgcaag gaagtgtaat cgagatgcag gagatcatgg gacgctacga
     1261 acggcttgcg gcccgggact cttgtgttgt ggttgaaggc gccggtggtc tgctcgtgcc
     1321 gatggggaag gattgggata tgcgtgactt gatcggccgg atcgggctgc ccgtcgtact
     1381 tgtggggcgg gccgggctag gcgggatcaa tcatgcgctg ctgacgctgg agggccttcg
     1441 gcaacgtcac tgtgcggtga tggctgtcgt gttgaacgag acggcgttgt ccaccacccc
     1501 cgcgcagcag gagcagtgtg cgtctacggt cgcgctgttg cgggagcgag ccggtgttcc
     1561 agtgttggga ccgctgccct atcagccaca acttggcact gattggtctg gcgccgtcga
     1621 ggcgctggca cagagcagcc cgatcaggga gcttgcgaat ctggtgtggg caaatgctgt
     1681 tggaatgttc ggatcgccgc gccgaggtcc ggagccttga ggatcgcaga gatcaccgcc
     1741 acgccatccg cccctgccgc cataatttca ccaaccgact cagccgtaat gccgccgatc
     1801 gcaaacatcg gcagcgtggt caacgcgcgc gccgcgcgta atccttccag accgaccacc
     1861 gggtcgtgat ccgcttttgt ggacgtactg aaaatcgggc cgaaccccac gtagtcgggt
     1921 tgtgcggtga cggcttcgcg cacctgagct gcattgtgcg tcgagagtcc gatgattttt
     1981 cccggcccca tgaggcgtct ggcgtaggag atgggaaggt cgtcctgccc caggtgcacc
     2041 ccgtccgcat tcacggccat cgccaaatcg caacgatcgt tgacgatcaa gagggcgccg
     2101 gcgtcggttg cggcctgacg cagcgcgagc gccttcgcat aggcgtctct catcgtggcc
     2161 tgcttgtctc gatattgaaa gagccggaca ccgtgcgcgg ctgcttcgcg caggagatcg
     2221 ggcagcgggc ggtccggccg gaccgtgggg tcgagcacga ggtataagcc cttgaggaac
     2281 ggttgtcggg tgggctgaga cgtgacgggc tccctgttta gcaggccgtt gaaaaagtcc
     2341 gccagcggcg ttctcgcgtc gctccatgcc tcaccgtacg acaggggtac gattcgccac
     2401 gtcgcttgct gcggccttgc cggacggcca ttttgaacgg cctgctggtg atccccatgg
     2461 atatatgaga tctgtttgtc agcaaactgt tacgaggact ggccggcaag aaagcggtcg
     2521 aggaacgtcg tggagacgtg tcccttctgg aaatcgggat cgttgaaaat tttccgatgg
     2581 agcgggatcg tcgtcttgat gccttcgatg acaaattcat cgagtgcgcg gcgcatgcgg
     2641 gccatggctt cctggcgatc acgtccgtgc gtgatcaatt tggcgatcag gctgtcgtag
     2701 tacgggacga ccacggcgtt cggttccatc gcggaatcga cgcgcacgcc aaagccgcca
     2761 ggggcggaat atttggtgat ctgccctgga cagggcgtga acttttccgg gtcttcggca
     2821 ttgatccggc actcgaagct gtgaccgttc aacttgatgt ccggctgctt gaacgagagg
     2881 ggcatgcccg aggcaatccg gatctgttct ttcacgaggt cgatcccggt caccatttcc
     2941 gtgatggggt gttcgacctg gatgcgggtg ttcacttcca tgaaaaagaa gttgcgatcc
     3001 ttgtcgagga gaaactccac tgtgccgacg ttgcaatagc gaatggcttt cacggcctgg
     3061 acggccaccc ggccgatttc gcgccgtaat ccttcatcga tggcaggcga cggggtttct
     3121 tccaccaact tttggtgacg tcgttgaatg gagcaatcac gctcgccgag gtggaccaca
     3181 tggccccgat tgtcggccac gatttgtact tcaatgtggc gcggttcgag gaaatagcgc
     3241 tcgagataga cgccgtcgtg gccgaaggtg gatttcgcct cggcttgcgc cgcttgaaac
     3301 gctcgcgcga gctcctccgg tttattcacc acgcgcatgc cccgtccgcc gcctccggcc
     3361 gaggccttga tgatcacggg gaagccgatg gtcttggcgg cctcttgcgc atcctgttcg
     3421 ctccggagtt cgccggggct gccgggggtg accgggaggc ccttcttggc gactacttcc
     3481 cgtgcttttg atttgtcgcc catcagcgcg atgttttcgg atgtcgggcc gatgaatttg
     3541 acgccgatcg attcgcagac ttccgcgaag tgcgcgttct ctgagaggaa tccatagccg
     3601 gggtgaatgg catcggcccc ggtgatttcg gctgcgctca gcacgttggg gatattgcgg
     3661 taactgaggg cggcttccgg cggtccgacg cagacgtgct cgtccgcagc ccgcacgtgc
     3721 aagcttgcgg catcggcttc ggagtggatc gcaacggttt tgatgccgag ctctttgcag
     3781 gcgcggatca cccgaagggc gatttctcca cgattggcaa ccaacacttt cttaaacaca
     3841 ggatactccg gcgcgggtca ctctctgtgt ccgcctggtt ctaaacggtt tgctctgcca
     3901 cgggtgagac ctgatccggt tctgttgcag gatcccggca tgtgccgtct gccgtcggac
     3961 ccacatgctt gaggaacgta tccccggatc gtctcgccat gaacccgcat gatctcgtcg
     4021 gctagggcgt ggcgttgggg tctatcaaga agagaggttg tccgtactcg acaggcttgg
     4081 tactctccgc cagtattttc gtgatccgtc cgtcggcctc ggactcgatt tcgttcataa
     4141 gcttcatggc ttcgacgata cagagcactt ggcccttcct cacgtaatcc ccctcttcca
     4201 catatgggtc tgcatccggt gagggcgagc gatagaaggt gccgacgata ggagacgtaa
     4261 tcgtgacctg gccatccgtt tctgcgggtg gacgggtctg cgccgccggc gtcgccccgg
     4321 ttgaggtcga ctggttcgtg gcggtctcca ccgtctgggc cgtcgtggtc cggattaccg
     4381 gctcatgtct gatgcggata cggactccgc tgcgctcgat ttccaactcg gtcagatggt
     4441 tgcgcttgag caggtcggcc aactgttgaa tctgcgcggc ctggtccgga gagagcagtg
     4501 tctcagcggc cgttgatcga ggcgcaaagg cgctcggcag gatgatcggc ttcccggccc
     4561 cccggccttt tcctgtcttt ttacctgatg agctcaacgc acgcgctcca caaattcacg
     4621 ggttctcgta tcgatgcgga tcgtttctcc gatttccagg tagagcggca ccttgatcgt
     4681 ggcaccggtc tcgacgatca ccggcttatt gccgcccgag gccgtatcgc cgcgcacgcc
     4741 cggttcaccg tcgaccactt taagttcgat gaagttcggc agcaggaccg taatcggccg
     4801 gtgctcgtag atcaggatct tggcgatcat gttttctttg agcagatcgg cg
//
LOCUS       DAORRQ010000143         4791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_557_length_4791_cov_9.927154, whole genome shotgun sequence.
ACCESSION   DAORRQ010000143 DAORRQ010000000
VERSION     DAORRQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_4791_cov_9.927154"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="PK224_20615"
     CDS             complement(<1..96)
                     /locus_tag="PK224_20615"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRB18208.1"
                     /translation="MKIAIIGGGPAGLMAAEAALAGGAHVALYDSM"
     gene            complement(100..519)
                     /locus_tag="PK224_20620"
     CDS             complement(100..519)
                     /locus_tag="PK224_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18209.1"
                     /translation="MIRPVVQEERTGCGIAAAAALAGVSYARAKAVAASLGISAQDPK
                     LWSETASVRRLLTQFGLHASRATKPFRSWTGLPDCGLLAIKWHLEQGRPFWHWVVFVR
                     ENERSYVVDSNPTLKAPLRTDFGRMRPVWFLTVTMKR"
     gene            complement(516..986)
                     /locus_tag="PK224_20625"
     CDS             complement(516..986)
                     /locus_tag="PK224_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRB18210.1"
                     /translation="MSIRLTHAQPSDAPLVADLVGELLQEIMTVIGVKAFEFSRVETE
                     ARAQRWLADGTYTVLLAYHDEKVAGFLALSESRSLYAEGAFGTIPELYVRPAFRSCNV
                     GTGLVAEAKKLSRARGWTRLEVTTPPLPQFDRTLAFYERQGFSISGGRKMKLSL"
     gene            complement(1158..1799)
                     /locus_tag="PK224_20630"
     CDS             complement(1158..1799)
                     /locus_tag="PK224_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18211.1"
                     /translation="MAGARRSKVVIVLFLVGLLCGMWSGPAHAEESGVNPDAKTEEPH
                     WVISIRGGVAQATQDHVSNAAASLGGAGSLQFLYQFSPYFRFGLTVDGQQQAVDVKNG
                     AGTFGDLLTVAVMPTVEMRPFQSTTAIPYLSVGLGMNFSMFSTATRVLPLDYGNTFAA
                     RIAGGLDIPLTPHWSLNTEVAWRLNKGEWTQSGATGRFDATSVLGLVGIRYMF"
     gene            complement(2095..3711)
                     /locus_tag="PK224_20635"
     CDS             complement(2095..3711)
                     /locus_tag="PK224_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HRB18212.1"
                     /translation="MESLHQLEVIIMLLAVVLALTTIAQKIRIPYPIFLVLGGLALGL
                     VPGLPAVTLHPDLVFLVFLPPILWAAAYFTSLREFRQNLRPISLLAVGLVLATSAGVA
                     WVAHALLPGIGWAEAIALGAIVSPPDAVSATAIGKRLRIPRRIVTILEGESLVNDATA
                     LVLYRAAVGAAMSGSFALGHTLFEFVFAALAGGVIGIGVAWLTRWALCATEDGFTQIG
                     VTLLAPYIAWVCGESVHASAVLACVAGGLYLRQSFSGAVSPATRLQARAVWDLLIFIL
                     NGVIFILIGLQLGALRDSVPPGQFGPVLIAGAWVSATAILVRLLWVPLAALIPRWLSA
                     KLRARDPMPPWSGLFLISWTGMRGIVTLAAALALPVATSTGVPFPFRSEIILISFTVI
                     LATLVLQGLSLPPIIRLLHLREDDGLEQEETLAREHAATAALNRLDQVVTENWVSLEQ
                     VERVRLRYLQRLERLTKASLAEETTLSSTTESVQRLQHEVLTAERVALIGLRDNGTIS
                     DEVLHRLEQELDVAALHLGVGERRPGRTPA"
     gene            complement(3780..4643)
                     /locus_tag="PK224_20640"
     CDS             complement(3780..4643)
                     /locus_tag="PK224_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRB18213.1"
                     /translation="MVRHGGFHAMLTAYNQVPIPSFMYGTAWKKEATTQLVQQAVATG
                     FTAIDTANQLIHYEEALVGEALVALAKQGTPRARLFLQTKFTPVNGQDHRTPYDAQAD
                     LTTQVTQSFASSLAHLHTDYLDSYVLHGPYGRRGLSDADWEVWAAIEALYDAGKTKMI
                     GISNVTADQLTLLCARAAHKPMVVQNRCYAALGWDQDVRTICRTHGIIYQGFSLLTAN
                     REMFADPEVRAMAVKYGMGLAQLVFRFAMQTGMLPLTGTTDPQHMQEDLRSDQFTIAP
                     DDLQRLEIIGM"
BASE COUNT         1050 a         1550 c         1375 g          816 t
ORIGIN      
        1 catcgaatcg tacagcgcga cgtgcgcccc gccggccaat gccgcctcag cggccatgag
       61 cccggccggc ccaccaccga taatcgcgat cttcacctgt tacctcttca tcgtcacagt
      121 cagaaaccac accggcctca tacgcccgaa gtcggtccga aggggagcct tcaatgtcgg
      181 gttggaatct accacgtacg accgctcgtt ctctcgcacg aacaccaccc aatgccagaa
      241 cggtcgtccc tgctccagat gccacttgat ggccagcaac ccacaatccg gcaaaccggt
      301 ccatgagcga aacggtttgg tcgcccgcga ggcatgcaga ccgaactgtg tcagcagccg
      361 ccgcaccgat gccgtctcag accaaagctt cggatcttga gcagagatgc ccaacgaagc
      421 ggcgacggcc ttcgcgcgcg cataggacac acccgccaac gcggccgctg ccgcaatgcc
      481 acagccggtt cgctcttctt gaacaacggg tcggatcaca gggacagttt cattttccgt
      541 cccccggaaa tgctgaatcc ctgtcgttca taaaacgcca gggtccggtc gaactgcggg
      601 agtggaggcg tcgtgacctc cagcctggtc catccccgcg cgcgggacag ctttttggcc
      661 tctgccacga gtccggtacc cacgttgcag gacctgaatg ccgggcgcac atacaattcc
      721 gggatggtcc cgaatgcgcc ttctgcatag agggagcggc tttccgacag ggccagaaaa
      781 ccggcaacct tctcgtcgtg atacgccaac aacaccgtat aggtcccgtc tgccagccat
      841 cgctgcgcac gcgcttcggt ctcgaccctg gagaattcga acgccttcac gccgatgact
      901 gtcataatct cctgcaacag ctcgccgacc aaatcggcca cgagcggcgc atcggacggt
      961 tgtgcatgag tgagacgaat cgacatgacg cgaaactacc cgcagcaggc tcgcgcgtca
     1021 agccgggtgg cttccgctgt cggtacggtg gactcaccga cagcggaggt cgccgaaaga
     1081 gaatcggatg aaggacagca gaacaaggcg ggataagcac atccggcatt cctgcggaac
     1141 gatcgccgga gcgagcgtca gaacatatat cgaatcccga ccaagccaag gacagacgtg
     1201 gcatcgaacc ggccggttgc gcccgattgg gtccactctc ccttattgag tcgccaagcc
     1261 acttccgtat tgaggctcca gtgtggcgtc aacgggatgt cgagtccgcc tgcgattcgc
     1321 gccgcaaacg tattgccgta atccagcgga agcacccgcg tggctgtaga gaacatgctg
     1381 aaattcatgc caaggccgac tgagaggtag ggaatcgcgg tggtggattg aaacggacgc
     1441 atttcgaccg tcggcatgac ggcgacggtc aacagatcac cgaaggtgcc cgccccattc
     1501 ttcacatcga ctgcttgctg ctgaccatca acggtcagac cgaaacgaaa atatggagaa
     1561 aattgataga ggaattggag gctgcctgcg ccgcccaacg atgcggccgc attggagaca
     1621 tggtcctgcg tcgcctgagc cacgcctcct cgaatagaga tcacccaatg cggctcttcc
     1681 gttttcgcgt ctggattcac accgctttcc tcggcatggg cgggtcccga ccacatgcca
     1741 cacaacagtc ccaccaggaa caaaacaatc acgaccttcg aacgccttgc gccagccatc
     1801 ctcccactcc ttactcgccg tgccctggct catgaagcgg gcacgagcac atgtgttcag
     1861 tgaaccccct gcctattgcc gagggcgcac tatccattag tcgccggaag ggaggaaagt
     1921 cctctgaagt gttaccgcga aggggggaac gaaagtggca gtcgcgcaac aaccggtcta
     1981 tgaacgaggg aacgaggtgg cgaagtcgac gagggaagag gagttgcagc cgttgtcacg
     2041 cccttacgcc cacggcattc ctgatcaaga gaaagtcatc caaacagacc tcgcttacgc
     2101 gggggttctg ccgggacgtc gctcgccgac acccaggtgg agcgcggcga catcgagttc
     2161 ctgttctaaa cgatgcagga cttcgtcgct gatcgtgccg ttatcccgaa gcccgatcaa
     2221 agcgacccgc tcggccgtga gcacttcatg ctgcaggcgc tggacggatt cggtggtcga
     2281 actcaaagtc gtctcctccg ccaaactggc cttggtgagc cgctccagcc gttggagata
     2341 ccgcaggcgc actcgctcca cctgctccag cgacacccag ttttccgtga cgacttgatc
     2401 cagccgattt aaggccgccg tcgccgcatg ttcccgtgcc agcgtttctt cctgttccaa
     2461 cccatcgtct tctctgaggt gcaacagcct gatgatcggc ggcaacgaga ggccctgcag
     2521 gaccagggtc gcaagaatca cggtgaagct gatcagaata atttcagagc gaaatggaaa
     2581 tggcacaccg gtgctggttg ccaccggaag cgccaacgca gcggcaaggg tcacgattcc
     2641 gcgcatgccg gtccaggaaa tgaggaacag gcccgaccat ggtggcatgg gatcacgtgc
     2701 acgtaatttc gcgctcaacc atcgcgggat gagagcggcc aacggcaccc acagaagccg
     2761 aaccaggata gcggtcgcgc tcacccaggc cccggcaatg agcaccgggc cgaactggcc
     2821 cggcggcacc gaatcgcgta acgcgcccaa ctgcaggccg atcaggataa aaatgactcc
     2881 attcaagata aagatcagga gatcccacac cgcacgcgcc tgcaatctgg tggccggaga
     2941 taccgcgccg ctgaacgact gacgaaggta cagcccaccc gccacacagg ccagcaccgc
     3001 agaggcatgc accgactccc cgcataccca ggcgatatac ggcgccagca gcgtcacgcc
     3061 gatctgcgta aacccgtctt ccgtcgcgca gagcgcccac cgcgtgagcc aggccacccc
     3121 gataccgatg acgcccccag ccagcgcggc gaatacaaac tcgaacagcg tatggccgag
     3181 tgcaaacgaa ccgctcatcg cggcgcccac cgccgctcga tacaacacga gggccgtggc
     3241 gtcgttgacc aggctttcac cctccaggat agtgacgatc cgtcgcggga ttctcagccg
     3301 tttaccgatg gccgtcgcgg acacggcgtc gggcggcgag acgattgcgc ccagcgctat
     3361 ggcctccgcc cacccgattc caggcaggag agcgtgcgcg acccaggcca ccccggcgga
     3421 ggtcgccagg accaacccca ccgccagaag cgaaatcggt cggagattct ggcgaaactc
     3481 gcgcagggac gtgaaatagg cggcggccca taagatcggc ggcaggaaga ccaggaacac
     3541 gaggtcagga tggagtgtca ctgcgggcaa gccgggcacc agacccagcg ccagacctcc
     3601 caacaccaga aagatcggat acgggatacg gatcttctgc gcgatggtcg tcagcgcgag
     3661 caccaccgca agcaacatga tgatcacttc gagttgatgc aggctttcca tcaatgcaca
     3721 agaccgtttc tgaggcgatc gcgcgggccg aaccaatcgc cgcggctaca ggcaaccggt
     3781 tacatcccga tgatttccag tcgctggaga tcgtcgggcg cgatcgtgaa ttggtcggag
     3841 cgcagatcct cctgcatgtg ttgtggatcg gtcgtaccgg tcagcggcaa catgccggtc
     3901 tgcatggcga atcggaacac cagttgcgcc agccccatgc cgtacttgac ggccatggcc
     3961 cgtacctcgg gatcggcgaa catctcccgg ttggccgtca gcagcgagaa cccttgatag
     4021 atgataccgt gagtccggca gatggtccgc acgtcctgat cccagcccag cgccgcataa
     4081 cagcgattct gcaccaccat cggcttatga gcggcccgcg cacaaagcag ggtgagttga
     4141 tccgccgtca cgttgctgat gccgatcatc ttcgtcttcc ccgcgtcgta cagggcttcg
     4201 atggcggccc agacctccca atcggcatcg ctcaacccgc gccgcccata ggggccgtgc
     4261 aagacatagg aatcgagata gtcggtgtgg agatgggcga gggaactggc gaacgattgt
     4321 gtcacctgcg tcgtaagatc ggcctgcgca tcgtaaggcg tgcgatggtc ctgaccgttc
     4381 accggcgtga acttggtttg cagaaagagc cgggcccgcg gcgtgccctg cttcgccagc
     4441 gccaccaacg cctctccgac caatgcttcc tcgtaatgga tcaactgatt agccgtatcg
     4501 atcgcggtaa accccgtggc cacggcctgc tgcaccagct gcgtcgtcgc ctcttttttc
     4561 catgcagtcc cgtacatgaa cgacggaata gggacctgat tgtaggcagt caacatggca
     4621 tgaaaccctc catgccgtac catacacaga tcctcgctcc ggtctcaatg ttccaaatgg
     4681 cccgcgaagt tgctagcccg gattattcat gaatgccgtc gcgaggcgtt cgagacggaa
     4741 gcccgccgag gcttctcgca cgtaaggccg acgcaggcgt acttgcagtc c
//
LOCUS       DAORRQ010000144         4560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_580_length_4560_cov_519.630633, whole genome shotgun sequence.
ACCESSION   DAORRQ010000144 DAORRQ010000000
VERSION     DAORRQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_580_length_4560_cov_519.630633"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="mnmA"
                     /locus_tag="PK224_20645"
     CDS             complement(<1..1044)
                     /gene="mnmA"
                     /locus_tag="PK224_20645"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRB18214.1"
                     /translation="MTRQTVLLGMSGGVDSSVAASLLVRQGYDVHGVTLQVWEHEDDQ
                     VAVSKRWEERGCCKIGIAKYVAQRLRIPYEVVDRREVFQQGVIDDFVAGYASGTTPNP
                     CVRCNERVKLRSLYALAHERGLDYVATGHYARVQQIDGQWSLHRALDARKDQSYFLYR
                     INPAWLPHLLFPVGHMQKCDVWQEAESLGLPVEELKESQEICFVSHGDYRTFIEQEMP
                     EAKKPGPFVGVDGEVLGQHEGIAFYTPGQRRGLGIAVGQRLYVQKVVPESGQVVLCAE
                     DQLVQSECQVADLSLFDHALGRQPVEADVKIRYATPPCPATLLPSESGTLQVQFHQPQ
                     RALSPGQSAVFYDG"
     gene            complement(1077..1532)
                     /locus_tag="PK224_20650"
     CDS             complement(1077..1532)
                     /locus_tag="PK224_20650"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRB18215.1"
                     /translation="MWGETKKQPVAEDDKFTFLGKGTSFKGIVTFDGTVRIDGRVEGE
                     VHTGGAVIVGESAVIKGVIAAGSVSISGRVKGSVTALQKVEIQKPGILIGDIQSPVIM
                     IEEGAHFHGMSNMGAEKWTEDESSEFGAPQDSGVRDLVAHRGKVKTQEP"
     gene            complement(1662..2150)
                     /locus_tag="PK224_20655"
     CDS             complement(1662..2150)
                     /locus_tag="PK224_20655"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRB18216.1"
                     /translation="MAWINKDKQDGKRQAGQSEGTEMENLESTAPREGNEEINAFVGK
                     GVSFKGVISYNGTVRIDGNLDGEIHTEGVLLVGEDAVLTAKITAGTVVCKGKITGDIS
                     AREKVKLRAPAVVNAGVKAPLLAMEEGVVFNGTLEMSQSGTREPQGQGSTRGQGVKLV
                     NG"
     gene            complement(2319..3167)
                     /locus_tag="PK224_20660"
     CDS             complement(2319..3167)
                     /locus_tag="PK224_20660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRB18217.1"
                     /translation="MEKKALGRGLDALLPTGRTTAEPERGDVQELRLEAIVPNRFQPR
                     QQFSEVELAELTASLKQNGLLQPILVRRKGDGIYELIAGERRLRAAKLAGMQKIPALV
                     RNVSDQESMVLALVENLQRDDLNPMETARAYQRMLNEFGLTQEAIAQKVACDRSSVAN
                     LLRLMSLPSEVQHMIESDQLSTGHAKVILGLMTPAAQVSLATQIVSGQLSVREAERLV
                     QEHAEARKPGKRPVRAPLRSDLEERLQKRLGTRVDVQKGRRGGKIVIHYFSPEELDGV
                     VERLLN"
     gene            complement(3145..3921)
                     /locus_tag="PK224_20665"
     CDS             complement(3145..3921)
                     /locus_tag="PK224_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRB18218.1"
                     /translation="MARIIAVANQKGGVGKTTTSVNLAAALAIEGGSVLLVDIDPQGN
                     ATSGLGVDAMSLTKTIYNALISKESIESLAMQTGVNGLSIVPANSHLAGAEVELVNME
                     DREQRLKEALAEVSARYDTILLDCPPALGLLTINAMVAAHSVLIPVQCEYYAMEGLGR
                     LMESIQRLRQSLNPGLEIEGIVLTMYDARNSLARQVVEQIRGHFGASVYQTMIPRNVT
                     LAEAPSYGRPALLYNMASAGAQAYLSLAKEFVVHGEKSPR"
     gene            complement(3985..>4560)
                     /gene="rsmG"
                     /locus_tag="PK224_20670"
     CDS             complement(3985..>4560)
                     /gene="rsmG"
                     /locus_tag="PK224_20670"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRB18219.1"
                     /translation="VSYFWRYLSELKKWNRAINLTAIDDDREILVKHFIDSIYGLKVI
                     DINSEIELLDVGAGAGFPALPLKFVRPQLSVELLEPSEKKGAFLRYVVGSLGLQNVTV
                     ATAKLEDYAGRGNVQGRFDYIVVRAFKVDQFGVALSTLLKPDGKLVLYRAEKVPRSFE
                     LVGLALIKEVEYELPARYGHRVLSVYSKALR"
BASE COUNT          978 a         1493 c         1112 g          977 t
ORIGIN      
        1 gccatcataa aacacggcgg actgacctgg gctcaaggcg cgctgcggct gatgaaattg
       61 aacctgaagc gtgccgcttt cagacggtag aagggtcgcc gggcagggag gcgtggcgta
      121 acgaatcttc acgtccgcct caaccggctg ccgaccgagg gcgtgatcaa acagactgag
      181 atcggcgacc tgacactctg actgcacaag ctgatcttcc gcacataaca ccacctgacc
      241 ggattcaggc accactttct gcacgtacaa ccgctgcccc acggcaatac ccaggccgcg
      301 gcgttgcccc ggcgtataga acgcgatacc ctcatgctgc ccgaggacct cgccatccac
      361 ccccacaaag ggtccgggct tcttagcctc cggcatttcc tgctcgatga acgttcgata
      421 gtcgccgtgg ctcacgaaac aaatctcttg gctttccttg agttcctcca cgggcaatcc
      481 gagcgattcg gcctcttgcc acacatcgca cttctgcata tggcccaccg gaaacagcag
      541 gtgcggcaac caggcaggat tgatccgata gagaaagtaa ctttggtctt tgcgcgcatc
      601 gagcgcccga tgcagcgacc actgcccatc gatctgctgc acgcgggcat agtgccccgt
      661 cgccacatag tccaggcctc gctcatgtgc caaggcatac agggagcgca atttcacccg
      721 ctcattgcac cgcacgcagg gattgggcgt ggtgccggag gcatacccgg cgacgaaatc
      781 atcgatcacg ccctgctgaa agacttcgcg acgatcgaca acctcatatg gaatacgtag
      841 acgttgcgcg acatacttcg cgatcccgat cttgcagcat ccccgctctt cccagcgctt
      901 ggagacggcg acctggtcat cctcatgctc ccatacctgg agcgtgacgc cgtggacatc
      961 gtacccctgc cgcacgagga gcgaggcagc gacggagcta tccactccgc cgctcatacc
     1021 tagcagtacg gtctgacgtg tcataaatgg taccggagga acaatgcgcc gagcacctac
     1081 ggctcttgcg ttttgacttt gccgcgatgg gctacgaggt cgcgcacgcc ggaatcctgc
     1141 ggagcaccga attcagagga ctcgtcctcc gtccactttt cggcgcccat attgctcatg
     1201 ccgtgaaaat gcgcgccttc ttcgatcatg atcaccgggc tctggatatc cccgatgaga
     1261 atccccggct tttgaatctc gaccttctgc agagccgtca ccgacccctt cacgcgtcca
     1321 ctgatcgaga cggatccggc agcaatcacg cccttgatga cggcgctttc tccgacgatc
     1381 accgccccgc ccgtatgcac ttccccctcg acacggccat cgattcggac ggtgccatca
     1441 aacgtaacaa tgcccttaaa gctggtcccc ttgccgagaa aggtaaattt atcgtcttcc
     1501 gcgaccggct gctttttggt ctcaccccac atcggcgtcc tccttcacac accccacaca
     1561 tcctagaggc cggggcgcag gctgaatgga accttgctat gcctacctct tgcttacaag
     1621 gcaagccaga ccctgtccaa caaattcgtc gttgagccga gttatccgtt gaccaacttg
     1681 acgccctgtc cgcgcgtgga tccctgcccc tgcggctcgc gcgttcccga ctggctcatc
     1741 tccaacgtgc cgttgaagac caccccttct tccatggcca acagcggcgc cttcacgccg
     1801 gcattcacca cggcaggcgc gcgcaacttc accttctccc gggcgctgat gtcgccggtg
     1861 atcttccctt tgcacaccac ggtaccggcc gtgatcttcg ccgtcagaac cgcatcctca
     1921 ccgaccagca acactccttc ggtatgaatc tctccatcca gattgccatc gatgcggacg
     1981 gtgccgttat aggaaatgac acccttgaag ctgacacctt ttccgacaaa ggcattgatc
     2041 tcttcgtttc cctcgcgcgg tgccgtagat tccaaatttt ccatttcagt tccctcgctt
     2101 tgtcctgcct gacgcttccc gtcctgcttg tccttattaa tccacgccat ttgttgcacc
     2161 tcccacctgt tacaacgtcg tccatgatcg gaccggcagg ctttcaatta cctgccggac
     2221 ggtcaccact aagagtgtcc ccatttaata tccggtgaaa atcgccgata ttttactctc
     2281 gcagcgagca gaaaatctcg cctaaaacac atctgccatc aattgagcag ccgctcgacg
     2341 acgccgtcca attcttccgg cgaaaaataa tggatgacga tcttgccgcc gcgccgcccc
     2401 ttctgaacat ccactcgggt ccccagacgt ttctgcaatc gctcttcgag atccgatcgt
     2461 aatggcgcac gaaccggacg tttccccggc tttctcgctt ccgcatgctc ctgtaccaat
     2521 cgctccgctt cgcggacgga cagttgcccg ctgacgatct gtgtcgccaa actgacctgc
     2581 gcggccggtg tcatcagccc gagaatgacc ttggcatggc ctgtggagag ctgatcggat
     2641 tcgatcatgt gttgcacctc ggagggcagc gacatcaatc ggagcaggtt cgccacggac
     2701 gaccggtcgc acgccacttt ctgagcgatc gcctcctggg taagtccgaa ttcattcagc
     2761 atgcgttggt acgcccgcgc ggtctccatg ggattcaggt cgtcccgctg gaggttctcc
     2821 accaacgcca acaccatcga ctcctgatcg gacacgtttc gcacaagagc tggaatcttc
     2881 tgcattcccg ctaacttagc agctctcaag cgtctctcac cggcaatcaa ctcatagatg
     2941 ccatcacctt ttcgtcgtac cagaatcggt tgcagcaagc cattttgctt caacgaggcg
     3001 gtcaactcgg ccagttcgac ctcggagaat tgctgccgtg gttgaaagcg gtttgggacg
     3061 atcgcttcga gccgcaactc ctggacatca ccacgctccg gctctgccgt tgttcggccg
     3121 gtcggcaata gtgcgtcgag tcctctaccg agggcttttt tctccatgga ccacaaactc
     3181 cttggctagg gaaagatagg cttgggcacc ggcggatgcc atgttgtaga gcagggccgg
     3241 gcgaccatag ctcggtgcct cggccaacgt cacgttgcgg ggaatcatcg tttggtacac
     3301 gctggccccg aaatgcccgc gaatctgttc gaccacctgg cgggccagcg aattgcgcgc
     3361 atcgtacatc gtgagcacaa tgccctcgat ttccaaccca gggttcagcg actgccggag
     3421 acgctgaata ctctccatca gccgaccgag gccttccatc gcatagtatt cgcattgcac
     3481 cgggatcaac actgagtgcg ccgcgaccat cgcgttgatg gtcaggaggc cgagggccgg
     3541 tggacaatcc agcagaatgg tgtcgtagcg ggcagacacc tcagccaggg cctctttgag
     3601 acgctgctca cggtcctcca tatttaccag ctcgacctct gcaccggcga ggtgggaatt
     3661 cgccgggaca atcgacaacc cgttcactcc ggtctgcata gccagagact caatactctc
     3721 cttactaatc aaggcattgt agatcgtctt cgtgagcgac atggcgtcca caccaagccc
     3781 gctcgttgcg tttccctgag ggtcaatatc gacgaggaga accgatcctc cctcgattgc
     3841 cagcgctgcg gcaaggttca cagaggtggt tgttttcccc actccaccct tttgattcgc
     3901 aacagcaatg attcgagcca tgtagttccc ctatccactc aaacaaagat atgtgcgccc
     3961 caatgttcca cgtggaacac catctcatct caacgccttt gaatacacgg acaacactcg
     4021 atgcccgtac ctagcaggaa gctcatactc gacttccttg atcaaagcaa ggccgacaag
     4081 ctcaaaactc ctaggaacct tctccgcacg gtataaaaca agctttccgt ccggcttcag
     4141 aagcgtcgac agcgccacgc cgaactgatc caccttaaag gccctcacga cgatgtaatc
     4201 aaaccgcccc tgaacatttc ctcgaccggc ataatcttcc aacttggccg tcgcaaccgt
     4261 cacgttctgc agaccaagcg atcccacgac atagcgcaaa aacgccccct tcttttcgct
     4321 cggctcaagc aattcaacgg acagctgagg ccgtacaaat ttgaggggca gagccggaaa
     4381 tccagcaccg gcaccaacat cgagcaactc aatttcacta ttaatatcaa tgactttaag
     4441 cccatagatt gagtcaataa aatgcttcac caggatctct cgatcatcgt cgatggccgt
     4501 gagattgatg gccctattcc attttttcag ctcgctgagg taacgccaaa aataagaaac
//
LOCUS       DAORRQ010000145         4488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_589_length_4488_cov_443.101060, whole genome shotgun sequence.
ACCESSION   DAORRQ010000145 DAORRQ010000000
VERSION     DAORRQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_4488_cov_443.101060"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PK224_20675"
     CDS             <1..457
                     /locus_tag="PK224_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18220.1"
                     /translation="ENLRISGQPKPHSPQSCPGRTVAATLSSMRLSGHIIGLLKEYMR
                     DLVEQARQESQAQEAFGFSAPPYQPDHAIADLLAILDDRIESEGVQVGLPIEFLHEMW
                     RRCNEARSEITDRVWLETNIGPSGPSKARVRELTYLSVIDCLERESSGE"
     gene            complement(550..1362)
                     /locus_tag="PK224_20680"
     CDS             complement(550..1362)
                     /locus_tag="PK224_20680"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRB18221.1"
                     /translation="MELTPMWFGVYKALKYLLYPLSWILIAGLLALLTACLPVSPGRR
                     TWVRRLLFLTVLLLLLTATPVLSLLYIALLETQYPSFQPTSTSKFDAVVVLAGGIFQK
                     GSLRPADEVSDASRQRTGCGSDLWQQNLAPKLLLTGGDATVFQTGLMESHEMKRWALR
                     LGVPESAILIEENSRTTYENAVQTKAMLGSGHILLVTAAYHLPRAVRLFEKQGFVVTP
                     FPCGYESQHTPQQAWEQATLFDFLPTAKALLITTQAVDEVAGIIVYWLAGKL"
     gene            1444..1929
                     /locus_tag="PK224_20685"
     CDS             1444..1929
                     /locus_tag="PK224_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRB18222.1"
                     /translation="MADGRHYWLMKSEPEVFSIDDLAGSPKSTTSWDGVRNYQARNFM
                     RAMKIGDQVLFYHSNANPPAVVGVAEVVRAAYPDATQFDKWDSHYDPASNPAQPRWDM
                     VDIRFVRKFLAPLSLDLLRAKAGLKGMELLRKGSRLSVQPVRAAEWNEIIRLEEVQAD
                     S"
     gene            complement(1985..2233)
                     /locus_tag="PK224_20690"
     CDS             complement(1985..2233)
                     /locus_tag="PK224_20690"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRB18223.1"
                     /translation="MVRIPNKVILPFGYHIMIRQVTDSEMDRQDSNADGIWDNEAKTI
                     YIRKRLPVTRRRYILAHELGHAWLDWQHRYLDDGKARS"
     gene            2449..3210
                     /gene="ricT"
                     /locus_tag="PK224_20695"
     CDS             2449..3210
                     /gene="ricT"
                     /locus_tag="PK224_20695"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRB18224.1"
                     /translation="MVYHAPMVEKLLAQELAKPYPAVVRIVGVKIRDRGEVKKFDAGE
                     ASLVMGDRVLLEVAGELSYGVVYGAPQVMPFIPPMRVLQPITRKATTEDVATIDRYER
                     LASEGMKACREQAAALGLRMKLVEVFCSFHRRQMTFVYTAEDRIDFRELVRLLARRFG
                     GRIEMRQVGVRDEASRLGGIDTCGLVLCCAAFLTEVKPVTVKQARVLGLPVDDPKLLG
                     VCGRLKCCLLFEAMDNPSSKSSALINPSRSRLASS"
     gene            3334..4425
                     /locus_tag="PK224_20700"
     CDS             3334..4425
                     /locus_tag="PK224_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRB18225.1"
                     /translation="MPQVLVLGAGKIGSLVAGLLSASGRYHVHLADLTLDAPKRLVDD
                     LSLSAVTPCALDVRRPDAVAAYVSSSPFDAVISSLPYFCNPTVAEIARAHQLHYFDLT
                     EDVAVTGRVKAISAGADHAFIPQCGLAPGFISIVTNDLIGHFESIDNVKMRVGALPVH
                     PSNALKYSLTWSTDGLINEYANVCVGIEGGEEVQLQPLEGYETIELDGLLYEAFNTSG
                     GLGTLADTYRGRVRTMNYKTLRYPGHCEKIQFLMKDLKLNEDRETLKRILERAIPQTQ
                     QDVVLIYAAVTGTRQGELFEETYVKKVYPRTILGRLWSAIQITTASSLCCVVDLVMAR
                     PSAYRGFVTQEHFHLQDVLNNRFGDCFRA"
BASE COUNT          924 a         1145 c         1378 g         1041 t
ORIGIN      
        1 agagaacctc agaatcagtg gtcaaccaaa gcctcatagc cctcagtctt gccccgggag
       61 gactgttgcg gctactctgt cgtcgatgcg tttgtcaggc cacattattg gattgctgaa
      121 agaatacatg cgggatcttg tcgagcaagc gcggcaggag tcgcaggctc aagaggcgtt
      181 cggcttctcg gcacctccgt accagccgga ccatgccatc gccgatttac tggctatttt
      241 agatgaccgc atcgaatcgg aaggggtgca ggttgggttg cccattgagt ttttgcacga
      301 gatgtggcgg cgctgcaacg aagcgcgcag tgagatcacc gatcgtgtgt ggctcgaaac
      361 caatatcggc ccgtctggtc cttccaaggc tcgtgttcgt gagctgacct acctctctgt
      421 gatcgattgt cttgagcgtg agtcttccgg cgagtgagtc agacgaacga cggcattccg
      481 tcgcacagct acacggccct tattcttttg gctgtgtgga gtctcagcag actagagtct
      541 tgggggatcc tacaatttcc cggcaagcca ataaacgatg atcccagcca cttcgtcgac
      601 ggcttgggtg gtgatgagca gggccttggc tgtgggaagg aagtcaaaga gggtggcctg
      661 ttcccaggct tgttgtggag tgtgttgaga ttcatagccg cagggaaacg gggtgacgac
      721 aaaaccttgc ttttcgaaga gtctgactgc gcggggcagg tgataggccg cggtcaccaa
      781 caggatgtgt ccggacccaa gcatggcctt ggtctgcacg gcgttttcat aggtggtcct
      841 ggagttttcc tcgatcagga tcgcggactc cggcacacca aggcgaaggg cccaccgttt
      901 catctcgtgg gattccatca gaccggtctg gaatacagtg gcgtcgcccc cggtgagcaa
      961 cagttttggg gcaagattct gttgccacaa gtctgagcca caacccgtac gctggcggga
     1021 ggcatcagaa acctcatcgg ctgggcggag tgatcctttc tgaaagatgc ctcctgccag
     1081 aaccaccacc gcgtcgaact tcgatgtgga agtgggctgg aatgatggat actgcgtttc
     1141 gagcagcgca atgtatagga gggatagtac gggagtggcc gtcagcagca acagtagcac
     1201 ggtgaggaac aagaggcgcc tgacccaagt cctccgcccg ggggacacgg ggaggcaggc
     1261 ggtcaacagg gcgaggagtc cggcgatgag aatccatgac aaggggtaga gcaggtattt
     1321 caaggcctta tacacaccga accacatggg cgtgagctcc atggacgctc ccggttaata
     1381 ggcgaaacag agtgctcagc atacactgag ggcgtacaga caccaagtgt tgaggaggat
     1441 tcgatggcag acggacggca ctattggttg atgaaatccg aacccgaggt cttttcaatc
     1501 gatgatcttg ctgggtcacc gaagagcacg acttcatggg atggtgtacg gaactaccag
     1561 gcgcgtaact ttatgcgggc aatgaaaatc ggggatcagg tcctctttta ccacagtaat
     1621 gccaatccgc ccgcggtggt tggagtcgcg gaagtggtga gagcggccta tcccgatgcc
     1681 acgcagttcg ataagtggga ttcgcactac gatccggcca gcaatccggc tcagcctcgc
     1741 tgggacatgg tcgatatccg gtttgttcgc aaattcctcg cgccgctgtc gttggacctg
     1801 ttgcgtgcga aggctggtct gaagggaatg gaattgcttc gaaagggatc gcggctgtcg
     1861 gttcagccgg tgcgggcggc cgagtggaat gaaattatca ggctggagga ggtacaggcg
     1921 gacagctagc acggtccggg aagaactcac ttccggacgg gaggtgtcct cgattcagca
     1981 cgggtcaact acgagccttt ccatcatcca gatatcgatg ttgccagtcg agccaggcat
     2041 gtcccagttc atgggcgagg atgtatctcc gccgcgtgac gggtagtcgt ttgcgaatat
     2101 agatggtctt ggcttcattg tcccaaatcc catccgcatt cgaatcctgc cggtccatct
     2161 cggagtccgt gacctgcctg atcatgatgt ggtagccgaa tggaaggatg actttgtttg
     2221 gtattcgcac catgcccctc acggtcgtat caaataggtg gcctcagcca agaattcctg
     2281 cttcggaata gcctaacata ttttctcttt cgtcttcctg tctaaataat cattactatc
     2341 agtggtttgc gagagattgc tgcttggtcg ctgtatggct gctcggtggt ggttgagcaa
     2401 ttttcgatgg cgatgagaca gcgctgagca tttcaattga aagattggat ggtgtatcat
     2461 gcccccatgg tcgagaaact tcttgcgcaa gaactcgcga aaccctatcc cgcagtcgtt
     2521 cggatcgtgg gcgtaaagat ccgtgaccgt ggggaggtga agaaattcga tgccggagag
     2581 gcgtccctgg tgatggggga tcgggtactg ctcgaagtgg ccggcgagtt gagctacggg
     2641 gtcgtttacg gggctcctca ggtgatgccg ttcattccgc ccatgcgcgt gcttcagccg
     2701 atcaccagga aggcgacaac cgaagatgtg gccacgatcg atcggtacga acggttggca
     2761 tcggagggaa tgaaagcgtg tcgggagcag gcggcggccc tgggactcag aatgaaactt
     2821 gtggaggtgt tctgctcgtt tcatcgccgg cagatgacct ttgtctatac tgctgaagat
     2881 cgaatcgatt ttcgcgagtt ggttcgcttg ttggcgcgcc gattcggcgg gcgcattgaa
     2941 atgcgtcaag ttggggtacg cgacgaagcc agtcggttgg gcgggatcga tacatgcggt
     3001 ctcgtactct gttgtgccgc gttcctcact gaagtgaaac cggtgactgt caagcaagca
     3061 cgggtgttgg ggcttcctgt cgatgacccc aaattactcg gtgtctgtgg tcgtctcaag
     3121 tgttgcctct tgtttgaggc gatggacaat ccttcctcaa aatcgtcagc tctgattaac
     3181 ccgtcccgtt cgcgcctggc gtcgtcgtaa tccacagcag ttcagaagaa tattctcctc
     3241 attcgtctcc ccggccccgc tctgtcctca cctttcgttc agaaaaggcg gttcttttcg
     3301 tccgaaggag tataatgggc tgtgaggtga cggatgcctc aggtgctggt gcttggagcc
     3361 ggaaaaatcg ggtccctcgt ggctgggttg ttgtcggcta gtgggcggta ccacgttcac
     3421 ctagccgatc ttacccttga tgccccgaag cgactggttg atgacttgtc gttgtccgcg
     3481 gttactccct gtgcgttaga cgtgcggcga ccagatgcgg ttgcggcata cgtcagttcc
     3541 tcccccttcg atgcggtgat ctccagcctg ccgtacttct gcaacccgac cgttgccgag
     3601 atcgcgcgag cccatcaact ccattatttc gacctgaccg aagacgtggc cgtgaccggt
     3661 cgcgtcaagg cgatcagcgc gggtgccgat catgcgttta ttcctcagtg cgggttagcg
     3721 ccggggttta tcagtatcgt gacgaatgac cttatcggcc attttgaatc catcgacaac
     3781 gtgaagatgc gtgtcggcgc cctgcctgtt catccgagta atgcgctgaa gtactccctc
     3841 acctggtcca ccgacgggtt gatcaatgag tatgccaacg tctgtgtcgg cattgagggg
     3901 ggggaggagg tccagcttca gccgttggaa ggctatgaaa caattgaatt ggacgggctg
     3961 ctctacgagg ccttcaacac gtccggcggc ctgggcactc tcgcggatac gtaccgcgga
     4021 cgggtccgaa ccatgaatta taagaccctg cggtatcccg ggcattgcga aaaaattcaa
     4081 tttctcatga aggacctcaa gctgaatgag gatcgggaga cgctcaagcg aatcctggag
     4141 cgagcgattc ctcagacgca gcaggacgtg gtgttgatct atgcggctgt gaccggcacc
     4201 aggcagggtg agttgttcga ggagacctat gtgaaaaagg tgtatccgcg gacgatcctt
     4261 gggcggctct ggtctgcgat tcagatcacg acagcgtcgt cgctctgttg cgtcgttgat
     4321 cttgtgatgg ccagaccttc agcttatcgt ggcttcgtca cgcaggaaca ctttcacttg
     4381 caggatgtgc tgaacaatcg cttcggggat tgtttccggg cctgacggca tcatgacgat
     4441 acaggaactg ttcaagacct tggggttgac gcaggaacag cccggggg
//
LOCUS       DAORRQ010000146         4404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_595_length_4404_cov_1962.082778, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000146 DAORRQ010000000
VERSION     DAORRQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_595_length_4404_cov_1962.082778"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PK224_20705"
     CDS             <1..568
                     /locus_tag="PK224_20705"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HRB18226.1"
                     /translation="KERALDHPSVENVTTYIKVQKEAYDRSQRFTDAWQIAMYTDPKL
                     DYASQHPTSTYGHSVEAEIKRNTEEQLLAGTADRVGLFFFFTSTCPFCQEQSKVLKVF
                     ADTYGLTVKPVSLDGVGLPEYPQPATNNGMAENVGVHMVPMIYLAIPEENFLKPLGAG
                     LMTNADLRERLLVLLRNRGLLGERRSQG"
     gene            579..1913
                     /locus_tag="PK224_20710"
     CDS             579..1913
                     /locus_tag="PK224_20710"
                     /inference="COORDINATES: protein motif:HMM:NF017892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HRB18227.1"
                     /translation="MRSKLILVGVCMWAVLVWPPLVQAQSMNDALTNMFTSWGVGITS
                     PPGAYESQSRGYFSGGGVSVRLWQDPMRLWSIAPPRLSVGCQGIDVYLGSFSYGKLDR
                     YVQLLQQIGTGAVLGYAFQLAMKAICEDCADVLNKIEAAARALNAAGRIQPCQTGIEL
                     GKALSGNEASKQKLASRFGDAWQKMKEAGGAIGDVFEDRDTVKNQSNADAASALKGTE
                     YDVTGNLVWDVLTQAGLDQNLIVMVMSVTGTVIVGNDGDVQTRDPTMTFEQLVDSHLG
                     DVVKVFNCGGDSECLNPTITDATDIEGFESRVYTSMSNLIEDLYSGSAISATDQQIIN
                     MTPVPILTMLADYGRPRDVGNQIARLSSEVVAADMGYQWVKWAATETSRNVSYIQKVK
                     PEWPGNLQDFQGRLHQILRGASESLAKRQRMVNNINQLMQLTKTQGEMRSLR"
     gene            1958..>4404
                     /locus_tag="PK224_20715"
     CDS             1958..>4404
                     /locus_tag="PK224_20715"
                     /inference="COORDINATES: protein motif:HMM:NF019530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HRB18228.1"
                     /translation="MWTIYTFGNGDVLTQILLGIRIMMTTGRFESLMELALLVFAMFG
                     LVYFVFNRRFLVPLVVGAFFIFFVSVRITVDVSVEDQVNPDVPDQIVNSVPLAIALPA
                     YLSGQVGYYTTQLVEASFPMPVQYTQGQASFNRPLFDLQKVLNARLQDSDLARNTDSY
                     FMMCVFREVGLLQKNLGTLMNSPNLLNDIGATNNALTALGYTGGTLEAAPRVCPDFYS
                     SLILPAYTTGQPDYEGAMRHLRTQLQLDPATGDEEGLTEDIRANLLLSVGQSARDMIN
                     NVLLIHAWKNAESADAKRRAATADTVLLQQQAIQKDLKFQSYSKSYVAQKVVPLLRTI
                     VEGVVYIMTPFLLVLAISPGMSRLMGTYWRLFMWLQTWSPLYAVLNFVMFWEARSRLG
                     AILSSNGVGQSVNYATLDRINEHVAMLNSTAADYVWLVPAIGFGLMWGGSQVLGSILA
                     GSRTAETVAGQVGGQFSRGLGANLEEGPSYRHEQRGAQFGEMIGHDVEPQMTVPTGQG
                     GFRVDSQSGTSEIHDSQGGITSIGANGIQNYKGPEGQYAMDSKGHLLSGVFRHKMFDP
                     QSGKQVDAQTSVFGSMVEHRFDTMGEDGSRKSVLVQENQDTGELVARTEQSVRKGLSE
                     TRTIAEDGSQSFARSGNVPLFLEVAGETRPDAGFVQMMGERGPGERVDTLTQSTFQST
                     MGAKQSYTGRLVERDGQFHFLATEGGAVRGFDNKDTEAGVHRESFVSPDGSNYFVETG
                     QAEQMVLGKDGVTRPMEGYVRAFGSVDRSDPVHPRDIYSSYSLTSDEGGHTHTVTGTL
                     GTDASGRQFLVEATEHF"
BASE COUNT          993 a         1084 c         1361 g          966 t
ORIGIN      
        1 gaaggaacgc gcgttggatc atccttcggt cgagaatgtg acgacttaca tcaaagtgca
       61 aaaggaagcg tatgaccgct cgcaacgctt caccgatgca tggcaaatcg ccatgtatac
      121 ggaccccaaa ctcgactatg cgtcgcaaca ccccacgtcg acctatgggc atagcgtcga
      181 agccgagatc aagcggaaca ccgaagagca gcttctggcg ggcacagccg accgggtcgg
      241 actgttcttc ttcttcacct cgacctgccc gttttgtcaa gaacagtcca aggtattgaa
      301 ggtctttgcc gatacctacg gactgacggt gaaacctgtg tcgctggacg gggtgggatt
      361 gccggagtac ccgcagcccg ccactaacaa tggcatggcg gagaatgtgg gcgtccatat
      421 ggtgcccatg atttatttgg caattcctga ggagaacttc ttaaagccgc tcggagccgg
      481 tctcatgacg aatgcggatc tacgggagcg cttgctggtg ttgttgcgta accgaggatt
      541 gttaggcgag cgacggtctc agggataaag gacacacgat gcgaagcaaa ctgattctgg
      601 tgggagtgtg tatgtgggca gtactggtct ggcctcccct ggtacaggcg cagtcgatga
      661 atgatgccct tacgaacatg ttcacgtcgt ggggtgtcgg gatcacgagt cctccaggtg
      721 cctacgaaag tcaaagtcga ggctacttca gcggcggcgg ggtctcggtc cgtttgtggc
      781 aggacccgat gcggttgtgg tcgattgccc cgccgcgatt gagcgttggt tgccagggca
      841 tcgacgttta cctgggctcg ttttcctacg gcaaactaga tcggtacgtc caattgctgc
      901 agcaaatcgg gaccggtgcc gtcttggggt acgctttcca gctggcgatg aaagccattt
      961 gtgaagactg cgcggacgtg ctcaacaaga ttgaagcggc tgcgagagcg ctcaatgctg
     1021 cgggacggat ccaaccctgt caaacaggga ttgaattggg caaagcgcta tccggaaacg
     1081 aagcctccaa acaaaagctc gcgagtcgat tcggcgatgc ctggcagaag atgaaggaag
     1141 cgggcggcgc cattggagac gtgtttgaag atcgggatac cgtcaagaac cagtcgaatg
     1201 cggatgcggc ctcagcattg aaaggcacgg aatacgatgt cactggcaat ctggtgtggg
     1261 atgtcctgac gcaggctggc ctggatcaga atctcatcgt catggtgatg tcggtcaccg
     1321 ggaccgttat agtcgggaac gatggcgatg ttcaaacccg cgatccgacg atgaccttcg
     1381 aacagctggt cgatagtcat ctgggcgacg tggtgaaagt gtttaattgt gggggcgact
     1441 cggaatgttt gaaccccacg attactgacg cgaccgatat cgaaggattc gagagtcgcg
     1501 tgtatacgtc tatgagtaac ctgatcgaag acctctactc cggctcagcg atcagcgcga
     1561 cagatcagca aatcatcaac atgaccccgg tccccattct gaccatgctc gccgattatg
     1621 ggcgtccacg ggatgtcggc aatcagattg cgcgattgtc ttcagaagtg gtcgctgcgg
     1681 acatggggta tcaatgggtg aagtgggcgg ccaccgaaac gtccaggaac gttagctata
     1741 tccaaaaggt caaacccgag tggccaggca atctgcaaga ctttcagggg cggctgcatc
     1801 agatactaag gggggctagt gagagtctcg cgaagcggca acgtatggtg aataacatca
     1861 accagcttat gcagttgacg aagacgcagg gcgaaatgcg atcgctgcgg tagtcggagt
     1921 aagtctgttt gcgatgtgag ggagaggata gcgcatcatg tggacgatct atacgtttgg
     1981 caacggcgat gtgctgacgc agattctgct gggcatccgg atcatgatga ccaccggacg
     2041 cttcgaaagc ttgatggagc tcgcacttct tgtcttcgcg atgttcggac tcgtgtattt
     2101 tgtgttcaat cggcgcttct tggtcccgct ggtcgtcggg gcatttttca ttttctttgt
     2161 cagtgtccga atcactgtcg acgtgtccgt ggaagaccaa gtcaatcccg atgtgccgga
     2221 tcaaattgtg aacagtgtgc cgctggcgat cgcgttaccg gcatacctct cggggcaagt
     2281 agggtactac acgactcagc ttgtcgaagc gtcgtttccg atgcccgtgc aatataccca
     2341 gggacaagcg tcattcaatc gcccgctatt cgatctgcaa aaagtcctca atgcgagatt
     2401 gcaagactcg gatctcgccc gcaataccga ttcatatttt atgatgtgtg tgtttcggga
     2461 ggtgggcctg ctccagaaga acctcgggac gctgatgaat tctccgaatc tgctcaatga
     2521 tatcggggct acgaataacg cactcacggc actcggatat acaggtggga cgttggaagc
     2581 ggccccgcgg gtgtgtcccg acttctatag ttctctcatc cttcctgcgt ataccacggg
     2641 gcaacctgac tacgaagggg cgatgcggca tttgcggaca cagttgcagc ttgatccggc
     2701 caccggcgat gaggaagggc tcactgagga catccgggcg aatctcttgt tatccgtcgg
     2761 gcaatcggcc cgagacatga ttaataatgt gctgctcatc catgcgtgga aaaacgccga
     2821 atccgccgat gccaaacggc gcgcggcgac tgcggacact gtgcttctgc agcagcaggc
     2881 gatccaaaag gacttaaagt tccaaagcta ctcgaagtcc tatgtcgctc aaaaggtcgt
     2941 accgctatta cggaccattg tggaaggggt tgtgtacatc atgaccccgt tcctcctagt
     3001 gcttgccatt tcgcctggga tgtcacggtt gatgggcacc tattggcgct tgttcatgtg
     3061 gctccagacc tggtcgcctc tgtatgccgt gctgaatttt gtcatgtttt gggaagcgcg
     3121 aagccggctc ggcgcgatcc tgagctcaaa cggggttggc caatccgtca actatgcaac
     3181 gttagacaga atcaatgaac atgtcgccat gctgaattcc acagccgcag attatgtgtg
     3241 gctcgtgcca gcgatcggtt tcggtctgat gtggggcgga agccaggtgt tgggatcgat
     3301 cctggcagga tcgcggactg cggaaaccgt tgcggggcaa gtgggaggac agttctcgcg
     3361 tgggcttggt gccaatttgg aggaaggccc gtcctatcga cacgaacagc gtggggccca
     3421 gttcggcgag atgatcggcc atgacgtcga accacagatg actgtgccga ctggtcaggg
     3481 tgggtttcgt gtggatagtc aatccggcac gagtgagatc cacgactccc agggcggcat
     3541 caccagtatt ggcgcgaacg gcattcagaa ctacaagggg ccagaaggac aatatgcaat
     3601 ggattcaaag gggcatctgc tgagtggggt gttccgtcac aagatgtttg atccacagtc
     3661 agggaagcag gtcgatgcac aaacctcggt gtttggttct atggtggagc atcgcttcga
     3721 tacgatgggg gaggatggct ccaggaagag cgtgcttgtg caggaaaatc aggataccgg
     3781 agaactagtg gcgcgcacgg aacaatccgt tcggaaaggc ctgtccgaga cgcgtacgat
     3841 tgctgaagac ggtagtcaaa gttttgcacg atcggggaat gtgccgttgt ttctggaagt
     3901 cgcgggcgag acacgtcccg atgccggatt tgtccagatg atgggtgagc gtgggccagg
     3961 cgagcgggta gacaccctca cacagtcgac atttcagagc acgatggggg cgaaacaatc
     4021 gtataccggg cgactggtag aacgcgatgg acagtttcat tttctggcga ccgagggcgg
     4081 ggccgtccga ggatttgaca acaaggatac ggaggccggc gtccacaggg aatcgttcgt
     4141 ttcccctgac ggttccaatt attttgtgga gactgggcaa gcggaacaga tggtcttagg
     4201 gaaagatggg gtgactcggc cgatggaggg atatgtcagg gcgtttgggt cagtcgatcg
     4261 ctcggacccg gtgcatccgc gagacatcta ttccagttac agtctgacca gtgatgaggg
     4321 tggtcacacg cacacagtga caggaacatt gggcaccgat gcatcaggac gacagttctt
     4381 ggtggaagcc acggaacatt ttga
//
LOCUS       DAORRQ010000147         4219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_619_length_4219_cov_15.925312, whole genome shotgun sequence.
ACCESSION   DAORRQ010000147 DAORRQ010000000
VERSION     DAORRQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_619_length_4219_cov_15.925312"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PK224_20720"
     CDS             complement(<1..885)
                     /locus_tag="PK224_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18229.1"
                     /translation="MSRTDILASWRQLTGRLSGQPATMPYAGDELPLRSELFSAEQMQ
                     QHGKTLAGLHAVSAEHEPDPLLARLAENEAVLLDVRNLVAEAVKGSRRITPAGEWLLD
                     NFYLIEEQIRLARRHLPKGYSRELPRLGNGPSAGLPRVYDIALEIISHGDGRVDPESL
                     SGFVAAYQTVTALKLGELWAIPIMIRLALIENLRRAGARIASDRVDRNRAHEWAGQMM
                     ETAEKDPKSLILVIADMARSNPPMVSAFVAELARRLQGQSAALALPLTWIEQRLSESG
                     LTIEQLVQSETQQQAIDQV"
     gene            complement(1748..2806)
                     /locus_tag="PK224_20725"
     CDS             complement(1748..2806)
                     /locus_tag="PK224_20725"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRB18230.1"
                     /translation="MNRPIDNQHVIEIKPLPSPRDVKTQLPISDEVSELVFQTRQAIR
                     NILHGRDMDRLIVIVGPCSIHDPDAAYEYADRLKPVADAVRDKLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIAQGIQLARTILLNINGKGLPCATELLDPVTPQYIADLLSW
                     TAIGARTTESQIHREMASGLSMPVGFKNGTEGSLQVAINAMITSRSPHHFVGVNADGI
                     TSIIKTTGNPDHHIVLRGGGGRTNYSVEDIARAEAAVASEGLARGVMVDCSHDNSGKN
                     HQRQVEVAGEVLKQFQQGRRAIMGLMLESHLQGGRQTWDPNKALTYGMSITDSCLAWS
                     DTEALLYGMAESLATRPV"
     gene            2980..3300
                     /locus_tag="PK224_20730"
     CDS             2980..3300
                     /locus_tag="PK224_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18231.1"
                     /translation="MFRHFAQKKSSSVVALLLACMLLAMAPFAAALEIHHELAAADHD
                     GHEHSDTDLCQWVQYHTGHSLVGDAPPPCSFLADAPPPPVYRSILSSQSLATSRTSRA
                     PPLT"
     gene            3405..>4219
                     /locus_tag="PK224_20735"
     CDS             3405..>4219
                     /locus_tag="PK224_20735"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB18232.1"
                     /translation="MLSSVRDVSQLCLGLLLMALALGLSPVFAQEPAVAKGRTITGTV
                     QNQDLRRVPQASVEVKDQEGTVVGAAVANEAGEFSVDLPSEGTYSVSAVQDTYRSEYA
                     IIKVGAEPAAPVTLTLSKTQEIALEVVSPLAPIQYKASSETYALSRKEIEALPRGNNN
                     ELHDVLLTIPSAVYGSLKQVHIRQDHANLQLRIDGVPIPDTVSSTFSDVISPRAWERA
                     DIILGGMEAQYGNKAAAVLDITTKSGTKPSFGSVQMMGGSNKTINPSFEYGGT"
BASE COUNT          773 a         1230 c         1341 g          875 t
ORIGIN      
        1 gacttgatcg atcgcctgtt gttgcgtttc cgactgcacc agttgctcaa tcgtgaggcc
       61 ggactcggac aggcgctgtt cgatccaggt gagcggtaag gccaacgccg cgctctgtcc
      121 ttgcagacgg cgggcgagct ctgccacgaa ggcgctcacc atgggcggat tggaccgcgc
      181 catgtccgcg atcaccagga tgagactctt ggggtccttt tcagccgtct ccatcatttg
      241 gccggcccat tcgtgggcgc gattgcgatc cactcggtcg cttgcgatgc gggcgccggc
      301 gcgtcgcagg ttttcgatca atgcgaggcg aatcatgata gggatggccc acaactcgcc
      361 gagcttcagg gccgtgaccg tctgataggc cgcgacgaaa ccgctgagac tttccgggtc
      421 cacccgtccg tcgccatgcg agatgatttc aagcgcgatg tcgtagaccc tgggcaagcc
      481 ggccgatgga ccgttgccca gccggggcag ttcgcggcta taccctttgg gcaggtgccg
      541 cctggccagg cgaatctgtt cttcgatcag gtagaagtta tcgagcagcc attctccggc
      601 cggtgtgatg cgtcgacttc ccttcaccgc ctcggccacc agattccgca catccagcag
      661 gacggcttca ttctcagcca gtcgagcgag cagcgggtcg ggctcgtgct cggcactgac
      721 ggcatgcaac cccgcgaggg tcttgccatg ctgttgcatt tgctcggcac tgaagagctc
      781 cgatcgcaac ggcagctcat caccggcata gggcatggtc gccggttgtc cgctcaggcg
      841 tccggtcaat tgacgccagc ttgcaagaat atctgtgcgg ctcatcttca aatttgagat
      901 tcccttcgat cgaggtcgcg gaggtggtct gcgggataca ggcaaaggac cggccctagc
      961 cggacggtcc ttgcgaatga aggtacggaa gcgggcacaa ggaggcagta gagcctcacc
     1021 cgcatgactc ataacggctt ctgctgcaat cgccgatctg tcgctgcgac agaccttcgt
     1081 cctgcggact cgcccctcac ccgctcaccg cactgcgcga gcgggcccgt ggtcttggga
     1141 ctccgtacgc ctggcgcagt gccgctcagt gactttgcgg cagaccgtca tgaatcatgc
     1201 gggccgagga atctgcaagc gaacgacgat tgtgcaatcg acaattcgga cacttcgatg
     1261 cgagctgtga cgcggatcga acgcagcgat aggaggcgtg ctggatgctg tcctactcta
     1321 gtccttttag gcaggtgaaa gacagacaaa tatccgcagg ctgtgcggct ggagcgtcga
     1381 agggagttct tgaggtgcac gacgagcacg ggagaaggct gagcagccgg cgctcagtca
     1441 gactcactgg ttggaggcgt agcgtggagg gcgttcctca gccgaggagc gagtcgtcga
     1501 aacgaatcag tcggtgaacg gtgggagtgt gcactcaaaa aattctgcgc agggcctccg
     1561 atcgaacatg ggtagcctgc aggctgttga gaaagcctgt ccggcaaggc cgcagcgagt
     1621 gaagaggcga atcgttcatt cgtacccgcc taccccgagc ctgccaagac aggctcttgt
     1681 cccgtggccg tacggtgagc ctctgagcga agcgagaacg ccgctggcgg gcgttgccag
     1741 cagcctgcta gacgggtcgc gtcgcgagcg actcagccat gccatagagc aacgcttcgg
     1801 tgtcgctcca ggccaggcag gaatcggtga tggacatgcc gtaggtcagg gccttgttgg
     1861 gatcccaggt ctgccggccg ccttgcaggt ggctttccag catgaggccc atgatggcac
     1921 ggcggccttg ctggaactgt ttgaggactt cgccggcgac ttccacctgg cgttgatggt
     1981 tcttgccgga attgtcgtgc gaacaatcca ccatcacgcc gcgagccaga ccttcgctgg
     2041 ctacggccgc ctccgcgcga gcgatatctt cgacactgta gttcgtccgt ccgccgccgc
     2101 cgcgcagcac gatgtgatga tcggggttgc cggtcgtctt gatgatagag gtgatgccgt
     2161 cggcattcac gccgacaaaa tggtgcgggc tccggctggt gatcatggcg ttgatggcga
     2221 cttgcaggct gccttccgtc ccgttcttga atccgacggg catggagagg ccgctggcca
     2281 tttcgcgatg gatctggctc tcggtcgtgc gcgcgccgat tgcggtccag ctcaacaggt
     2341 cggcgatgta ttgcggggtg accgggtcga gcagttccgt cgcgcagggg agacctttgc
     2401 cgttgatgtt caagagaatt gtccgggcca gctggatacc ttgggcaatg tcgcaggtgc
     2461 cgtccagatg gggatcgttg atcaggccct tccagcccac cgtggtgcgg ggcttctcga
     2521 aataggtgcg catcacgatc aggagtttgt cgcggacggc gtcggcgacc ggtttgaggc
     2581 ggtcggcata ttcataggcg gcgtcgggat catgaatcga gcaggggccg acaatcacga
     2641 tgagccggtc catgtcacgg ccgtgcagga tgttccggat cgcttgtcgc gtctggaaca
     2701 ccagctcgga cacctcgtcg ctgataggca gctgcgtttt gacatcgcgg ggcgagggga
     2761 gcggtttaat ctctatgaca tgctgattat cgatcggcct gttcatgctt atccctttga
     2821 aaggtctgtc tagttcacgc gtcgattcaa gattgccaaa caagatatcg aattctatag
     2881 gataaagtcc agtatggacg gttgtaggag gatcggcttg cattcgaggg ggccctttgt
     2941 tacaactcac gcctcacgaa tctgtcggcc aggacggtca tgtttcgtca tttcgcccag
     3001 aaaaaatcca gctcggtcgt cgcgcttctc cttgcgtgta tgctgctcgc gatggcgcca
     3061 tttgccgcgg cgttggagat tcatcacgag ctggcggcgg cggaccacga cggccatgag
     3121 cattccgata ccgacctctg ccagtgggtg caatatcaca cgggccattc gctggtgggt
     3181 gacgcgccgc cgccctgctc gttcctggcg gacgctccgc cacctcctgt ctatcgatcg
     3241 atcctcagtt ctcagtctct cgcgaccagc agaacctcgc gcgcgccgcc tctgacctaa
     3301 cctcaacgcc atcgcaacgt tcattttggc tttcaccgct tcgtctcagc cacggctttg
     3361 tgtcgggctt tgacctgtgc gggattattt ggggggaggt tcgcatgctg tcgtcggtca
     3421 gagatgtgtc tcaactgtgt cttggattgt tgttgatggc tcttgcgctc ggtctttcgc
     3481 cggtgttcgc gcaggagccg gcggttgcca aagggcgaac gattaccggg acggtgcaaa
     3541 atcaggatct ccggcgggtg ccccaggcct cggtggaggt aaaggaccag gaagggacgg
     3601 tcgtgggcgc ggcggtcgcg aacgaggccg gagaattttc tgtcgatctg ccgtccgagg
     3661 gaacctattc cgtgagcgcg gtgcaagata cctatcgcag tgaatatgcc attatcaagg
     3721 tcggggccga gccggccgct ccggtgaccc tgaccctctc aaagacccag gagatcgcgc
     3781 tcgaagtcgt gtctccgttg gcgcccattc aatacaaagc ctccagcgaa acctatgcgc
     3841 tcagccgcaa ggagatcgag gccctgccgc gcgggaataa caatgaactc cacgatgtgc
     3901 tcctcacgat tccgagcgcg gtctacgggt cgctcaagca agtgcacatt cgccaggacc
     3961 atgccaatct gcaactccgc atcgacggcg taccgattcc ggacacggtt tcgtccactt
     4021 tttcggatgt gatcagtccc cgggcctggg agcgggcgga catcatcctg ggcggcatgg
     4081 aagcgcaata cgggaacaag gcggcggcgg tgctggacat cacgacgaag agcggcacga
     4141 agccgtcctt cgggtcggtg cagatgatgg gcggctcgaa caagaccatc aatccgtcct
     4201 tcgagtacgg cggcacgat
//
LOCUS       DAORRQ010000148         4211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_621_length_4211_cov_27.045958, whole genome shotgun sequence.
ACCESSION   DAORRQ010000148 DAORRQ010000000
VERSION     DAORRQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_4211_cov_27.045958"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PK224_20740"
     CDS             <1..584
                     /locus_tag="PK224_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18233.1"
                     /translation="TFATASAIACALTISPAWANPEGGYGGHSGGYEKGQHGMGAAMM
                     EMMMHGGAGHLIRHLLKHEKDIGLSADQVAKLKDLQLNLDKNRIKMEADIQVAEREVK
                     ALNDDEKSNLSAIEAKLKQSADVQIGLRVLSIKTRREALALLTPEQRAKEQAEHEKMM
                     QQHKGAGQAAPHGKNPHSSNPHGSNPHGEAKPQ"
     gene            617..976
                     /locus_tag="PK224_20745"
     CDS             617..976
                     /locus_tag="PK224_20745"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRB18234.1"
                     /translation="MIKHVAVPALMVAALCFTSTPAWSDKGHKGKDEKCDAAELVKDA
                     KVTIDQAIKTALDAVPGTAVEAELEKKHDKTVWEVEILGADGKMTEVHIDAGTGTIID
                     KEAKQEKHEKKGKKEKH"
     gene            1206..2879
                     /gene="ilvD"
                     /locus_tag="PK224_20750"
     CDS             1206..2879
                     /gene="ilvD"
                     /locus_tag="PK224_20750"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRB18235.1"
                     /translation="MTIDPRHKSHNLLDGPGRAPARAMLKAVGFTDADLERPLIGVAN
                     TWIEVMPCNFHLRRLSERVKAGIRAAGGTPIEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDGVVALSGCDKTIPGTVMALCRLNVPSLMIYGGSIMPGQFQGHD
                     VTIQDVFEAVGKHAAGTMTNAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGISPM
                     GRNGVPAMDQRKDDVAFECGKLVMDLLKKDIRPKQIITRRSIENAIAAVATTGGSTNA
                     VLHLLAVAREMGVRLSIDDFDKINRKVPLLADLKPGGRFTAADLYAAGGTTLVAKRLI
                     DAKILHPDQITVTGRTIGEEAKAATEKPDQQVLRPLSKPIKPTGGLVILKGNLAPEGC
                     VVKVAGHSILHFSGPAKVYEREEDAFKAVQAGKIKAGDVVVIRYEGPSGGPGMREMLG
                     VTAAIVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAIKGGPIAAVKTGDIITFDIT
                     KRRLDVNVTQKELAARLKKVKHPSPRYLSGVMGKYARHVSSASEGAVTT"
     gene            2882..3790
                     /locus_tag="PK224_20755"
     CDS             2882..3790
                     /locus_tag="PK224_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18236.1"
                     /translation="MGILLRWSLAATMFVAACFVEAGRSVGAAERSEAACADWHSRHP
                     EWLWCDDFEVDRLESYFEYDIRHGRFVREAGAGVGHSTGMRAEYLPGDPHGGSLHLAF
                     GKTPSAYIKPVDAGTATYRDIYWRFDLRLQPGWTGGGADKLTRATILSSDRFAQAAIG
                     HLWSGSSPDSDPERLYLDPASGTDEFGVLRTTTYNDFLRLRWLGAAGGQTSLFSPPNI
                     GKWLCIEVHMKLNNGDADDGVLEYWIDDRLDAQRTGINWIGTYRDYGINAVYLEQYWT
                     SVPFAQIQQRYFDNFVVSTARIGCAL"
     gene            3893..>4211
                     /locus_tag="PK224_20760"
     CDS             3893..>4211
                     /locus_tag="PK224_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRB18237.1"
                     /translation="MSSPQTPSETQPHASTPKARAHRPPTSDLSRPEWYLNRELSLLE
                     FNRRVLDLAKDQKVPLLERLKFLCIVSSNLDEFFEVRVAGLKEQVTHGVEQRGADGLT
                     PSEL"
BASE COUNT          928 a         1323 c         1246 g          714 t
ORIGIN      
        1 tcacatttgc caccgcttcg gccatcgcct gcgcgctgac gatctcccct gcctgggcga
       61 accccgaagg tggatatggc ggacacagcg gcggctacga gaagggccag cacggcatgg
      121 gcgccgcgat gatggaaatg atgatgcacg ggggcgccgg gcacctgatt cgccacctcc
      181 tgaagcatga aaaggacatc ggattgagcg ccgaccaggt cgccaagctc aaggacctcc
      241 agctcaacct cgataaaaac cggatcaaga tggaagccga catccaggtc gccgaacggg
      301 aagtgaaggc actgaacgac gatgagaagt cgaacctgtc cgccatcgaa gccaagctca
      361 agcaaagcgc cgacgtgcag atcgggctcc gcgtcctgtc gatcaagacc agacgagaag
      421 ccctcgcgtt gctgaccccg gaacagcggg ccaaggagca agccgaacat gagaagatga
      481 tgcagcagca caagggcgcg ggccaggccg ctccccacgg caagaatccg catagcagca
      541 acccacatgg cagcaatccg cacggtgagg cgaagcccca ataaccgtgc acgttgcaca
      601 gaaccggagg tcacacatga tcaagcacgt tgcagtaccc gccctgatgg tcgcggcact
      661 ttgtttcacg agcactcctg cctggagtga caagggacac aaaggcaagg acgagaagtg
      721 tgacgccgcc gaattggtga aggatgccaa ggtcaccatc gaccaggcca tcaagaccgc
      781 gttggatgcg gttccaggca cggccgtcga agccgagctt gaaaagaagc acgacaagac
      841 ggtctgggaa gtcgaaatac tgggagccga cggcaaaatg acggaagtcc atatcgacgc
      901 gggcaccggc accatcatcg ataaggaagc caaacaagag aagcacgaga agaagggcaa
      961 gaaagaaaag cactaaggtt cctgcggcag ggtcgcgcct tcagatgcga tcctgccgca
     1021 ccgtctcgac ccgtcccatt catctgcccc cctttccccc tgcttgccat tcccaagcct
     1081 agcggcagcc gatctcagga ccagcggatt gaccctctga gcctatctat gcgaaagttt
     1141 acgatccatc agaacgcgcg ctgtgcgaag cggtaccccc cgttcccgta aatgaaggag
     1201 tttccatgac gatcgaccca cgacacaaaa gccacaactt gctcgacgga ccaggccgcg
     1261 caccggcccg cgccatgctc aaggccgtcg gcttcaccga tgccgacctc gaacgccctt
     1321 tgatcggcgt cgccaacacc tggatcgagg tcatgccctg caatttccac ctccgccgtt
     1381 tatcggaacg ggtgaaggcc ggaattcgcg cggcaggcgg caccccgatc gaatacaaca
     1441 ccatcgcggt gtcggacggc atttccatgg gcaccgaagg catgaaggcc tcgctcatca
     1501 gccgggaagt cattgccgac tccatcgaac tggtcgcacg tggacacttg ttcgacggag
     1561 tcgtcgccct gtccggttgc gacaagacga ttcccggcac cgtcatggcc ctctgccgct
     1621 tgaacgtccc gtcactcatg atctacggcg gctccatcat gccgggacag ttccagggac
     1681 acgacgtcac gattcaagac gtgttcgaag cggtcggcaa acatgcggcc ggcaccatga
     1741 cgaatgcgga actcaaggat ctcgaagacc atgcctgtcc gggccccggt gcctgcggcg
     1801 gccagttcac cgccaacacc atggccatcg ccttcgaatt tctcggcatc tcgccgatgg
     1861 gccgaaacgg cgtcccggcc atggatcagc gtaaggacga tgtggcgttc gaatgcggca
     1921 aactggtcat ggacctgctc aagaaagata ttcggcccaa gcagatcatc acgcgccgct
     1981 ccatcgaaaa tgcgatcgcg gcggtcgcga cgaccggtgg atcgacgaat gccgtcttgc
     2041 acctgttggc cgtggcgcgg gaaatgggtg tgcggctcag catcgacgat ttcgacaaga
     2101 tcaaccggaa ggtgccgttg ctggccgatc tcaaaccggg cggccgattc accgcggcgg
     2161 acttgtatgc cgccggtggc acaaccctgg tcgccaaacg tttgatcgat gcgaagatcc
     2221 tgcatccgga tcagatcacg gtcacgggac gcacgatcgg tgaggaagcc aaagccgcca
     2281 cggagaagcc ggaccaacag gtgctgcgtc cactgtccaa gcccatcaag ccgacgggag
     2341 gtctggtgat cctcaagggc aacctcgctc cggaagggtg cgtcgtgaaa gtcgccggcc
     2401 actccattct gcatttcagc ggaccggcca aggtctatga gcgcgaggaa gatgccttca
     2461 aggccgtgca ggccggaaaa atcaaagccg gtgatgtggt cgtcattcgg tacgaagggc
     2521 cgtccggtgg accgggcatg cgggagatgc tgggggtcac ggccgccatc gtcggagcag
     2581 ggctcggcga ctccgtcgcc ctgctcacag acggacggtt ctccggagcc acgcatggct
     2641 taatggccgg acatgtcgcg ccggaagcca tcaagggcgg tccgatcgcg gcggtgaaga
     2701 ctggcgacat catcaccttc gacatcacga agcgacgcct cgacgtcaac gtgactcaga
     2761 aggaactggc cgcgcgcctg aagaaggtga agcatccgtc ccctcggtac ctgtccggcg
     2821 tcatggggaa atacgcccgc cacgtctcct ccgcatcgga aggtgcggtg acgacctagt
     2881 catgggcatc ctgcttcgat ggagtctcgc ggcgacgatg ttcgtcgccg cctgcttcgt
     2941 cgaggcaggc cgttccgtcg gtgcggctga gcgttccgaa gccgcttgcg ccgactggca
     3001 cagccgtcat ccggaatggc tctggtgtga cgatttcgaa gtcgaccggc tagagagtta
     3061 tttcgagtac gacatccggc atggtcgttt cgtccgcgag gccggagcag gagtcggcca
     3121 ctcaaccggc atgcgcgcgg aatacctgcc cggcgatccc cacggaggct ccctccacct
     3181 ggcattcggc aaaactccca gcgcctacat caaaccggtc gatgccggga ccgcaacgta
     3241 tcgcgacatc tattggcgat tcgatctgcg acttcagcca ggctggaccg gcggtggcgc
     3301 cgacaaattg acgagagcca caattctctc cagcgaccgg tttgcgcagg ccgccatcgg
     3361 ccatctctgg agcggaagct cacccgattc agatccagag cggctttatc tcgatccggc
     3421 gtccggcacc gacgaattcg gtgttttgcg cacgaccacg tataacgatt tcctccgcct
     3481 acgctggctc ggcgcggcag gcgggcagac ttccctgttc agcccgccca acatcgggaa
     3541 gtggttgtgc atcgaagtcc acatgaagct gaacaacggc gatgccgacg acggcgtgct
     3601 tgagtattgg atcgacgatc gtctcgacgc gcaacgcacc ggaatcaatt ggatcggcac
     3661 ctatcgcgac tacggcatta acgcggtgta cctggagcag tattggacct ccgtcccgtt
     3721 tgctcagatt caacagcgat acttcgacaa cttcgtggtc tccaccgctc gcatcggatg
     3781 cgccctgtga caccggttgc cacctggata gtccgggcag catggtgaaa aatttaaccg
     3841 tttcttgatc cgccgtttcg cacggctgag atacactgcc gacccactgg ttatgtcatc
     3901 gccgcagaca ccttcagaaa ctcagccgca cgcatcgacg ccgaaggcga gggctcaccg
     3961 gccgcccacg agtgacctct cccggccgga atggtacctg aaccgcgagc tcagcctact
     4021 ggaattcaac cggcgcgtcc tcgatctggc gaaagatcag aaggttccac tgctggaacg
     4081 attgaagttt ctctgcatcg tgagctccaa tctcgacgag ttcttcgaag tccgcgtggc
     4141 aggactcaag gaacaggtga cccatggagt cgaacaacgg ggcgccgacg gactgacccc
     4201 gtcggaactc c
//
LOCUS       DAORRQ010000149         4173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_626_length_4173_cov_11.407479, whole genome shotgun sequence.
ACCESSION   DAORRQ010000149 DAORRQ010000000
VERSION     DAORRQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_626_length_4173_cov_11.407479"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1903
                     /locus_tag="PK224_20765"
     CDS             140..1903
                     /locus_tag="PK224_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRB18238.1"
                     /translation="MTPEQPSSATSTELLSTDPVDRVIAYHVRTKHHFNRYARSLGYL
                     DWANQPNPFRRFDGTQLVRLPLLKADEEPRSPSYDAIYQPGSVPVQPLTNRTVSRFFE
                     LALGLSAWKKAGESEWALRNNPSSGNLHPTEGYVLLPPTEGLDLKPGLYHYAPREHGL
                     ELRADCSPAAISSLLAPFPSGALLFGLTSVHWREAWKYGERAFRYCNHDVGHAIGSAR
                     IAAATLGWRMALLDGADQNQTARVLGTHRVDDFSGVEPEHPDCLAVIWPVEAEARASS
                     SSRENQNLPLFLEEAAVTGVAVGPWHGKANELSREHGVHWDVIDQVAEASWKTSLEHP
                     IVSLAGTPIVPPGTLHASRTTDDAAAIIRQRRSAVSFDGRTSISAATFFHILQRVMPR
                     VERPQLQRPMPWDVLPWDPAIHLMLFVHRVEGLEAGLYMLARDPNKLPLLQQSMNPEL
                     EWTSAPGCPEDLPLFWLLQGNAQRLAAQVSCQQGIAGDSAFSLGMLAEFEGRLRQGGA
                     WWYPRLFWETGLLGQVLYLEAEAAGVRGTGIGCFFDDPVHEVVGIKDLSIQSLYHFTV
                     GGPVDDQRLMTLPPYHHLRHE"
     gene            1963..2820
                     /locus_tag="PK224_20770"
     CDS             1963..2820
                     /locus_tag="PK224_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18239.1"
                     /translation="MFKIKKKIEKPKPPILYNVIEDYSEFDAPGNVTACAMTLPEDLA
                     AHAKILAESYDVPLEQMTQLLAEGGVYVYPQVGGLLTRGLFACRIDATGATPKQTWVL
                     DLMQFAEAEQWARLRAVPLIEAATEVFYRTLPEELKTKLAKKNLGLDYRTLDRAVAKG
                     GDIKFIDFRKDWSPHFKRLCIMPDGRLVETGGLEEFAQLHQITVSQAKKLVEEGGTMD
                     VAGGEVLACQIVNGQPAVARFSAKNYTKAKELAASKGLHILDALSEVAYNDSVMMRTL
                     QRKDLGGRI"
     gene            2905..3321
                     /locus_tag="PK224_20775"
     CDS             2905..3321
                     /locus_tag="PK224_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HRB18240.1"
                     /translation="MKQTILIGVASLLLAGCAGPKPILYPNDHYRQVGADASESDIED
                     CMTLAEQAGASPSEGRTAQVATGTVAGGAIGSAAGAVGGAILGHPGRGAMVGAASGAT
                     AGFLRGLFRRSPPSGAFTNYVDRCLRDRGYDPTGWQ"
     gene            complement(3324..3722)
                     /locus_tag="PK224_20780"
     CDS             complement(3324..3722)
                     /locus_tag="PK224_20780"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRB18241.1"
                     /translation="MLIVLVGILPLHNFVGHAHWQYIRWVPTPDQLSSPAILLDLGID
                     AVANILLFLPFGLLYTLRGLGGKSFSPGHLVLMAFALSFSIEYYQVYCHNRSTSLLDL
                     LDNVLGAYIGMKAGEAYVRRYMARVVEPAA"
     gene            complement(3897..>4173)
                     /locus_tag="PK224_20785"
     CDS             complement(3897..>4173)
                     /locus_tag="PK224_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB18242.1"
                     /translation="AKDGREALDLYQKHKADLLIMDLLMPEVDGLEATLQLTREYMDT
                     KIIAMTGAQGDRNFLDIAKLFGAHRTFEKPFDLKEMLNAVEAELAQK"
BASE COUNT          819 a         1159 c         1352 g          843 t
ORIGIN      
        1 aagcgagggc ggcagtcgcg taaccacgtg tccacgaaat cgggggaaga tcaagtcaca
       61 tatgtctgtc agttgaccaa ggctgaagct agtcccaacg cgtttacttt ccttcctccg
      121 cctcgttaga ctggccgcca tgactcctga acaaccgtct tccgccacct ccactgagct
      181 gctttcaacc gatccggttg atcgcgtgat tgcctatcac gttcgaacca aacaccattt
      241 caatcgatat gcgcggtcat tggggtatct ggactgggca aaccagccca atccctttcg
      301 gcgatttgac gggacgcagt tggtgcgctt gccattgctg aaggcagacg aggaaccacg
      361 gtcgccgtcc tacgacgcca tctatcaacc ggggtctgtt cctgtgcagc cgctgaccaa
      421 caggaccgtt tcacggtttt tcgaactggc cctcgggctc tcagcctgga agaaggccgg
      481 ggagtcggaa tgggccctgc ggaacaatcc ttcgtccggc aatctccacc cgacggaagg
      541 ctacgtcctc ctgccgccga ccgagggtct ggacctgaag ccggggctct atcactacgc
      601 accgagagaa catgggctgg aattacgcgc ggactgttcg cccgccgcca tctcgagtct
      661 attagctccc tttccgtcag gagcgttgct gttcggtctc acctctgtcc attggcggga
      721 agcctggaag tacggcgagc gggcgtttcg ctactgcaat cacgatgtcg gccacgcgat
      781 cgggtcggcg cgtattgcgg cggcgacgct cggttggagg atggcgctgt tggatggagc
      841 cgatcagaat cagaccgcga gggtactggg aacccaccgc gtagacgatt tcagcggggt
      901 ggagccggaa catccagatt gtctcgcggt gatctggcct gttgaagccg aggctcgtgc
      961 atcgagctca tcgcgtgaga atcaaaattt gccgttgttt ctggaggagg cggccgtcac
     1021 cggtgtggct gtggggccgt ggcatgggaa ggccaatgag ctgagtcgcg aacatggggt
     1081 gcattgggac gtcatcgatc aggtggcaga ggcctcttgg aagaccagcc tggagcaccc
     1141 gatcgtgtcg cttgctggga cccccattgt cccacccggg acgcttcacg cttcacgaac
     1201 gacggacgac gcagcggcga tcatcaggca gcgccgcagc gccgtctcct tcgatggccg
     1261 cacctctatt tcagccgcca cattctttca catcctgcag cgggtgatgc ctcgcgtgga
     1321 gcgaccgcaa ttgcagcggc ccatgccgtg ggatgtcctg ccttgggatc cggcgattca
     1381 tctcatgttg ttcgtgcatc gcgtcgaagg gctggaggcg ggtctgtata tgctggcgcg
     1441 tgacccgaac aagctgccgc tgctgcagca gtcgatgaat cctgagttgg aatggacctc
     1501 ggcgcccggc tgcccggaag atcttcccct gttctggctg ttgcagggca acgcacaacg
     1561 actggcggcg caagtcagtt gccaacaggg cattgccggc gatagtgcct tttcgctcgg
     1621 gatgctagcg gagttcgaag gccgcttgcg gcagggcggg gcttggtggt atccacgcct
     1681 gttctgggaa acgggcctgc tcgggcaggt gctctatctg gaagcggagg cagcgggagt
     1741 gcggggcacc ggcatcggtt gctttttcga cgacccggtg catgaggtcg tgggcatcaa
     1801 ggacctgtcc attcaatcgc tgtatcactt tacagtcggc gggccggtgg acgaccagcg
     1861 gctcatgacg cttcctccct atcatcacct ccggcatgag tgattgcgca ggacctgctt
     1921 tgaacgagag acgaacgacg agatacgagg aacgaactca atatgtttaa gatcaagaag
     1981 aagatcgaga agccgaaacc gccgattttg tataacgtga tcgaagatta ttcggagttc
     2041 gatgcgccgg gtaacgtgac ggcctgcgcg atgacgttgc cggaggacct ggcagcgcat
     2101 gcgaagatcc tggccgaaag ttacgacgtg ccgttggagc agatgacgca gctgctggcg
     2161 gagggcggcg tctatgtata tccccaagtg ggcggattac tgacgcgtgg cctctttgct
     2221 tgccggatcg atgccacggg cgccacgccg aaacagacct gggtgctcga cctgatgcag
     2281 tttgccgagg cggagcaatg ggcccggttg cgcgcggtgc cgttgatcga ggcggcgaca
     2341 gaggtgttct atcggacgct tcccgaggaa ttaaagacca agctggcaaa aaagaatctc
     2401 ggcctcgatt accggaccct cgaccgtgcg gtagcgaagg gcggcgacat taagttcatc
     2461 gacttccgca aagactggtc tccccatttc aagcggctct gcatcatgcc cgatggacgt
     2521 ttggtggaaa cgggcgggct ggaggagttt gcgcagctgc accagattac ggtgtcgcag
     2581 gccaagaaac tcgtggagga gggtgggacg atggatgtgg ccggcggaga agtgctggcc
     2641 tgtcagatcg tgaacggtca accggcagtc gcccgcttca gcgcgaagaa ctacacaaaa
     2701 gcgaaagaat tggcggcgag caaagggctg catattctcg atgccttgag cgaagtggcc
     2761 tataacgatt cggtgatgat gcgaacgttg cagcggaagg atctgggggg acggatttaa
     2821 gtctcagcag gcatcaggct tccgccgatg cggccggtca accgtgcctc cttcgatgcg
     2881 tgtggaacag aggacctgtg agccatgaag caaacgatcc tcatcggtgt cgcgagtctg
     2941 cttctcgccg gttgtgcggg gccgaagccg attctctacc ccaacgatca ctatcgacag
     3001 gtcggcgccg atgcgtccga atcggatatc gaagactgca tgaccttggc cgagcaagcc
     3061 ggggcctctc ccagcgaagg aagaaccgcg caagttgcca ccggcacggt ggcgggcggg
     3121 gcgatcggat cggccgccgg tgcggtgggc ggagcgatcc tgggacatcc ggggcgtgga
     3181 gccatggtcg gcgcagccag cggcgcgacg gccgggtttc tgcgaggcct gttcaggcga
     3241 tcgccgccga gcggggcgtt taccaactat gtggatcgtt gtctgcgtga tcggggatat
     3301 gatccgacag gctggcaata atcttatgcg gccggttcca ccactcgtgc catatatctc
     3361 ctgacgtacg cttcccccgc tttcatcccg atatacgcac ccagaacatt gtccagcagg
     3421 tcgagaagcg atgtgctgcg gttgtgacaa tagacctggt agtactcgat gctgaaggac
     3481 agtgcgaagg ccatcagcac aaggtggccg ggagagaagg atttgccgcc caatccgcgc
     3541 agggtataaa gcaggccgaa ggggaggaag aggaggatgt tggccacggc atcgatgccg
     3601 agatccagga ggatggccgg cgaactcagc tggtccggtg tcggcaccca gcgaatgtat
     3661 tgccaatgcg cgtggccgac aaagttatgc agcgggagga tacccacgag cacgatcagc
     3721 atgctccagg cacaccagag acgccatgcg cgagtggggg ttagaaacac gggtgtttgg
     3781 ctgtggctgt ccgctgcacc gttcgattct tgcagaagca gcggtggggt ttccagcaga
     3841 ttttccttcg tgaacggctt ccgggggaag ggcaggggcg cggaggggag tgcggattac
     3901 ttctgagcca gttccgcttc cacggcgttc agcatttcct tcaagtcgaa gggtttttcg
     3961 aaggtccggt gggcgccgaa gagtttcgcg atatccagaa agttccggtc cccttgcgcg
     4021 ccggtcatcg cgatgatttt cgtgtccatg tattcgcggg tcagttgcag ggtggcttcc
     4081 agtccatcga cctccggcat gagcaggtcc atgatcagca ggtcggcttt atgtttttga
     4141 tagagatcca gcgcctcccg cccatctttc gcc
//
LOCUS       DAORRQ010000150         4160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_627_length_4160_cov_5.487942, whole genome shotgun sequence.
ACCESSION   DAORRQ010000150 DAORRQ010000000
VERSION     DAORRQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_627_length_4160_cov_5.487942"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>227
                     /locus_tag="PK224_20790"
     CDS             <1..>227
                     /locus_tag="PK224_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18243.1"
                     /translation="FATNVLTWLTQLFITNRVVARYGLVASLLFLPVVSLIGFLGIAL
                     WPGLVVYVSFSVLRRVGEYALSKPAREVLFT"
     assembly_gap    228..246
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <247..464
                     /locus_tag="PK224_20795"
     CDS             <247..464
                     /locus_tag="PK224_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18244.1"
                     /translation="EKYKAKNFIDTAISRAGDASTGWLVSGIKALGVTTAQISWVLVP
                     LMFFWAWLGRWLAGQVPPGPSVERGDI"
     gene            615..1979
                     /locus_tag="PK224_20800"
     CDS             615..1979
                     /locus_tag="PK224_20800"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRB18245.1"
                     /translation="MVEVPVKLYLDKVLKSAREAVRPFALMTGPARDKALRGMATAIA
                     EAEEDILAANEKDVDAVGKSMTGYENRERVRDAVARVRMTADDVKAMVDRLHRIADLP
                     DPLGEVLGRHDEPNGLQVSRVRVPIGVIGIVSELAPLETIDALALCLKSGNVCVFRGS
                     PDWTLTQQTIAACLSKAAADAGVPLGACTIIERPEKEAALELIKSGKALDAIIPRGGA
                     GLRKVVQEQAKMPILCHDGGITHVYIDGDVDIPLAQNIVVNSKVQQPLAANALDTLLV
                     HQGIARPLLSALILRLLDEFKIDVLGCPKTVALMGQMLMTGHKAVKPAQDADWNRQFQ
                     GPTMAIKMVPSFDDALAHITQHGPSHTCVIVTKSYESAMRFTKEVDAGSVLVNASSRL
                     NAGDSLGFGADVGLSSSRHHARGPIGLTQLTCEKYVVFGSGQLRHPHPVPLAYEDAIM
                     LKRP"
     gene            1990..3243
                     /locus_tag="PK224_20805"
     CDS             1990..3243
                     /locus_tag="PK224_20805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRB18246.1"
                     /translation="MNVSREEDPVPGDTNDEIRASLREHLAWWGLRHVESDAAYFAWQ
                     RDVFSPQELTTLHRHIEAKRQATDGPAAEIAFYDLTAQPRSVPALYSQRYEYYLEVGA
                     RVAGHLGDASTILDVGCGIGILTTFYAKRCPASTIVGIDRSPASIDLARQRAQELGLT
                     NVRFECRDLDQQDLAGCFNLIIATHTLLQAEQEPGLPSRDWSTFARSADAQAQQGFEQ
                     RIGVGVRLDRLRAGLTPGGRMVVFEKTRQLARRIPFQRALAARGLRLLAPPEPVRYRS
                     VEEVTDDGPLYVMGTVPREQACGWDESPEADGMPPLHLDSLRSARAQGDQPLYENHQI
                     SAEQGWLSLPAKQVTAQVTSTEPDGRQLHVEWGRAGAFAYLYCANTFDQRQLVLIEPA
                     RAAVLETYYREITREQPAGGKEPLR"
     gene            3240..4091
                     /locus_tag="PK224_20810"
     CDS             3240..4091
                     /locus_tag="PK224_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18247.1"
                     /translation="MTPSISRPESSSSAAQEVVGLVPAAGLAKRLQPFPCSKEVYPVG
                     FVSDRQTGRPRPKVAAHYLLEKFRAAGITKAYLVIRDGKWDIPNYFREGGVVGLSLAY
                     IVIAGSLGPPDTIDRAYPFLEQKRVAFGFPDILFGPDDAYRQLIAAQERTGADVVLGL
                     HRVYDHRVWDMVDCDADGLVRNIVMKPVTTTLTYGWCCAVWTPAFSEFLHRFLGAEET
                     HRNLAQLANTAHDLGGDLAVGVVLQAALKAGLLLQSVIFPHDTYIDIGTPEDLAKAVR
                     QFNPPER"
BASE COUNT          779 a         1197 c         1314 g          851 t
ORIGIN      
        1 atttcgcgac gaatgtgctc acctggctga cgcaattgtt catcacgaat cgtgtggtgg
       61 cccggtacgg cctggtggcc tcgctcctgt tcctgccggt cgtcagtctg atcgggtttc
      121 tcggcatcgc cctgtggccg gggctcgtcg tgtatgtgag tttttccgtg ttgcgccggg
      181 tgggggaata tgccctctcc aaacccgccc gcgaagtgct cttcacgnnn nnnnnnnnnn
      241 nnnnnnaaga gaaatacaaa gcgaagaatt tcattgatac ggcaatctcg cgcgcagggg
      301 atgcctctac cggatggctc gtatcgggca tcaaggccct cggcgtaacc acggcacaga
      361 tttcctgggt gctggtgcca ctcatgttct tttgggcctg gctcggccgc tggctcgccg
      421 ggcaggtacc tcccggccct tcggtcgaga gaggcgacat ctgacggccc ctccgtcttt
      481 gactcacaaa aaaatcgcat ggtatagtcc ggcgcgacgc agtgaccatt ttctgatctg
      541 ctgatgcggc ggccgattgc ccgctgagaa catcgccgaa tccgccgggt attaagtcga
      601 aagataggaa caccatggtt gaagttcccg ttaagctgta tctcgataaa gtattgaaat
      661 cagcccgtga agccgtgcgt ccttttgccc tcatgacggg tccggccagg gataaggcat
      721 tgcgggggat ggcgaccgca attgcggagg cagaagagga catcctggcc gccaatgaaa
      781 aggatgtgga tgcggtcggg aagtccatga cggggtacga gaaccgggaa cgggtccgag
      841 acgcggttgc gcgggtccgt atgacggccg acgacgtcaa ggccatggtg gaccggttgc
      901 accggatcgc cgatctgccg gatccgctcg gtgaggtgtt gggacgtcac gatgaaccca
      961 acgggctcca agtcagccgc gtgcgtgtgc cgatcggggt gatcggtatt gtgtcggaac
     1021 tggcgccgct cgaaacgatc gacgctctgg cgctctgctt gaagtccggt aacgtctgtg
     1081 tgttccgcgg ttccccggat tggacgctga cgcaacagac cattgccgcc tgtctctcga
     1141 aggccgccgc agatgctggg gttcctctcg gcgcctgtac gatcattgag cgtccggaga
     1201 aggaggcggc gctggaactg atcaagtccg gcaaggcact ggatgcgatc attccgcgcg
     1261 gcggcgcggg gcttcgtaag gtggtgcagg agcaggccaa gatgccgatc ctctgccacg
     1321 acggcggcat tacgcatgtg tacatcgacg gtgatgtcga cattccgctg gcccaaaata
     1381 tcgtggtgaa ctccaaagtg cagcagccct tggcagccaa cgcgttggat acgctcctgg
     1441 tgcatcaggg gatcgccagg ccgctgttgt cggcgctgat tctgcggttg ctcgatgaat
     1501 tcaagatcga cgtgctgggc tgtccgaaga ccgtggcact catgggccag atgctcatga
     1561 ccggacacaa ggccgtcaag ccggcgcagg atgcagattg gaatcggcag tttcaggggc
     1621 ctaccatggc gatcaaaatg gtgccgagct tcgatgacgc gctggctcat attacgcaac
     1681 acgggccgag tcacacctgc gtcatcgtga cgaaatcgta tgagtccgct atgcgcttca
     1741 ccaaagaggt cgatgccggt tcggtcctgg tcaacgcctc gtcgcgcctg aatgccggcg
     1801 acagtttggg atttggcgcc gatgtcgggt tgagttcctc gcgccatcac gcgcgagggc
     1861 cgatcggtct gacacagctc acttgcgaaa agtatgtcgt cttcggcagc ggtcagctcc
     1921 gtcaccccca tccggtgccg ctggcctacg aggatgccat catgctcaag aggccgtaac
     1981 gcgagtatcg tgaacgtctc tcgtgaagag gaccctgtgc ccggcgacac gaatgacgag
     2041 atacgagcga gcctgcgtga gcacttagcc tggtggggac tccggcacgt cgaatccgat
     2101 gcagcctatt ttgcctggca gcgggacgtc ttttccccgc aagagctcac gacacttcat
     2161 cgtcacatcg aagccaaacg gcaggccacc gacggtcctg ccgctgaaat cgcgttctac
     2221 gatctgaccg ctcagccccg ttctgttccc gcgctctaca gccagcgata cgaatattat
     2281 ctggaggtcg gggcacgggt ggccggtcat ctcggcgatg cctcgacgat tctcgatgtc
     2341 ggctgcggca tcggcattct cacgaccttc tacgcgaaac ggtgcccggc atcgaccatt
     2401 gtcgggatcg atcgctcgcc ggcctccatc gaccttgccc ggcagcgggc gcaggagctg
     2461 ggcctgacca atgtacgctt tgaatgccgc gatttagatc aacaggatct cgccggctgt
     2521 tttaacctga tcattgccac ccacacgttg ctgcaggctg agcaggagcc gggtctgccg
     2581 agtcgcgact ggagcacgtt cgcacgctca gcagatgcgc aggcccagca gggattcgaa
     2641 cagcggatcg gcgtgggcgt caggctggac aggcttcgtg ccgggctgac tcccggtggt
     2701 cgcatggtgg tcttcgaaaa aaccagacaa cttgcccgcc gcattccatt tcagcgcgcg
     2761 cttgccgctc gcggccttcg attgctcgca ccgccggagc cggtgcgcta tcgatcagtg
     2821 gaggaggtca cggacgacgg tccattgtat gtaatgggaa ccgttccccg ggagcaggca
     2881 tgcgggtggg atgaatcgcc ggaagcggat ggaatgccgc cgctccatct ggactcgctc
     2941 cgctcggctc gggcacaagg agaccagccg ctgtacgaaa atcatcagat ttcggcggaa
     3001 cagggatggc tctcgttacc ggcaaaacag gtgactgctc aggtcaccag cacagagccg
     3061 gatggtcgac agctgcatgt cgaatggggg cgggcagggg cgtttgcgta tctgtattgc
     3121 gccaacacct tcgaccagcg tcaattggtc ctgattgagc cggcccgcgc tgctgtgctg
     3181 gagacctact atcgggaaat caccagggaa cagcctgcgg gtgggaagga gcccttgcga
     3241 tgacgccttc gatcagccgg ccggaatcgt cttcatcggc cgcacaggaa gtggtcgggc
     3301 tggtgcccgc agccgggctg gcaaaacgct tgcaaccctt cccctgcagt aaggaggtct
     3361 atccggtcgg cttcgtgtcg gatcggcaaa ccgggaggcc tcgtccgaag gtcgcggcgc
     3421 attatctttt ggagaagttc agggctgccg ggattacaaa ggcctatctg gtgattcgag
     3481 acggcaaatg ggacattccg aattatttcc gcgagggcgg agtggtgggg ttgtcgctcg
     3541 cctacatcgt gatcgccggc tcgctgggac cgccggatac catcgaccgc gcctatccct
     3601 ttctggaaca gaagcgtgtg gccttcggat ttcccgatat tctttttggc ccggacgatg
     3661 cctaccggca gctgattgcg gcgcaagagc gaaccggagc ggatgtggtc ttggggctgc
     3721 atcgggtgta tgaccatcgc gtctgggaca tggtggattg cgacgccgac ggcctcgtgc
     3781 ggaatatcgt gatgaaaccg gtgacgacga cgttgaccta cggttggtgc tgcgcggtct
     3841 ggacaccggc cttttcggaa tttctccacc gctttctggg cgcagaggag actcaccgca
     3901 atttggctca attggccaat accgcccatg atctgggcgg ggatttagcc gttggggtgg
     3961 tactgcaagc cgctttgaaa gcggggctgt tgctgcagag cgtgatcttt ccccacgata
     4021 cctacatcga tatcggcacg ccggaagatc ttgccaaagc ggtgcgtcag ttcaacccgc
     4081 ccgaacgata actccgacac ttacggatag gccgccagga gccgctcggc aatcctgagg
     4141 cgattctgat cctcctggtt
//
LOCUS       DAORRQ010000151         4128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_630_length_4128_cov_6.455684, whole genome shotgun sequence.
ACCESSION   DAORRQ010000151 DAORRQ010000000
VERSION     DAORRQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_630_length_4128_cov_6.455684"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="PK224_20815"
     CDS             complement(<1..>1399)
                     /locus_tag="PK224_20815"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB18248.1"
                     /translation="VYVSGLIPGQEGSFRSPEETIDIAVPILDDHQHAVIGAIKASYR
                     FDSLFAMIKEIRIGQTGHAMLFDAAGQPLVCPILPRRAHRIPSQLMAMIVSSNPGWGI
                     AEDDGHGATDTVVGYAPVTGLSLPDNSWHVFVRQQPEETYAPIRDQLRNLALIGLVML
                     CLLWAMGRYVAARIARPIQVLKSGVEAISQGTYDGPLDIKTGDEFEELAVAVHRMADR
                     LQSSRTELESLNAELVNRVEEKTAEITRQMRKLELSERLATLGKVASGIAHEINNPLG
                     IILNRVECMEADAAQAGVPEEVGRDLVTIRTQAERISRVTRSILTFSRGTVSMLKPLD
                     LNCVARTCVAMAGERVAALAVRIDAQLAPEVSPVMGDRDRLETVLLNLINNAIDAVTG
                     SDSQGIITVSTARMPIDGDEWVRISVSDNGPGVPTEILDRIFDPFFTTKPAGQGTGLG
                     LFLSYGIVSDHRGRIE"
     assembly_gap    1400..1433
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1434..1945)
                     /locus_tag="PK224_20820"
     CDS             complement(<1434..1945)
                     /locus_tag="PK224_20820"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18249.1"
                     /translation="MTIRTIKGRIVLAIVLVGCIPLVIGLVLASMSGMRSLRDVIGGN
                     FQAIAEQAADRLTMLVQSEVQGVRLLASAPLRVRQPVEAANLSYKGEWADSQRLIQER
                     AKEWEKGHDSAAGLLNSELSRFLLETKVRDGDKMVGLLITDRYGALVAASSEPDHYFL
                     SQESWWEALQA"
     gene            complement(2021..2815)
                     /locus_tag="PK224_20825"
     CDS             complement(2021..2815)
                     /locus_tag="PK224_20825"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRB18250.1"
                     /translation="MSRGGAERIVRRAHRWLGIGALGFLLLSVVTGLLWADAKFLYWD
                     EHYKEKVRPLTGPPVDSATLPLDRALVLAKEAVGGRALVEQATLRTDFGRLFYEVKLR
                     VEGVSTTLLLDAMNGERLSPITSELAPTIAAQYVRSPATVVGVEIERYTPRKKKRAYD
                     AARVRFDDDGATEIVLDRQTGEILEDEGRWRKVHFAVMQLHQLNFFGFEKTLLNIPGV
                     PILLMSLSGLLLWGFHRARARRAAVDGLAAAVSSRVPTAPEETTHA"
     gene            complement(2816..3625)
                     /locus_tag="PK224_20830"
     CDS             complement(2816..3625)
                     /locus_tag="PK224_20830"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HRB18251.1"
                     /translation="MNRRRCAVGLWSVMLLSMIVGPSPATAHFVWVEADAIAPAETGQ
                     PLKIYFGEYSEFLREERGGRLDSIDGVTLRVQDSKQGTTEVALTKQVNHFAGALPSCL
                     PGRNAVVAQQAQAPVQDLRKHDIGIVKPMFYARTYFMCLEEGRINEHERDQATPMALD
                     MIPLTKGLNLATGRVSHVPGGEIVVKGFFKGQFLPNATILVHAPIGWDKELKTDGEGI
                     VAFTPLWPGRYVLELIHVEKTPGEFQGKTYEALRHRSTLAIQVRAERSGEK"
     gene            complement(3675..4031)
                     /locus_tag="PK224_20835"
                     /pseudo
     CDS             complement(3675..4031)
                     /locus_tag="PK224_20835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT          819 a         1344 c         1146 g          785 t
ORIGIN      
        1 cctcaatgcg gccacgatgg tccgagacaa ttccataact cagaaacagg cccagcccgg
       61 tcccttgacc ggccggtttg gtggtaaaga acggatcgaa gatgcgatcg aggatctcgg
      121 tcggcacacc gggcccgtta tcggacacgc tgattcgcac ccactcgtct ccgtcgatcg
      181 gcatccgcgc agtgctcacc gtgataatcc cttgactgtc ggagccggtg actgcatcga
      241 tggcattgtt gatcagattg agcaggaccg tttccaggcg atcgcggtcg cccatcaccg
      301 gtgatacctc gggcgccagt tgcgcgtcaa tccggactgc cagcgccgca acccgttcac
      361 ccgccatagc gacacaggtg cgcgccacgc agttgagatc caggggcttc aacatcgaga
      421 cggtgccgcg ggagaacgtg aggatactgc gcgtgacccg tgaaatccgt tccgcctgcg
      481 tccggatcgt caccagatcc cgtccgacct cttccggcac gccggcctgc gccgcatcgg
      541 cttccataca ttcaacccgg ttcaaaatga tcccgagggg attgttgatc tcatgggcga
      601 tgccgctggc gactttgccg agcgtggcca ggcgttcgga caactccagt ttccgcattt
      661 gacgggtaat ctccgccgtc ttctcctcca cacgattcac cagctccgca ttcaacgatt
      721 ccagttcggt tcgtgaggat tgcaaccggt ctgccatccg gtgcacggcc acggcaagct
      781 cctcaaactc atcccctgtt ttgatgtcga gcggaccatc gtacgtaccc tggctgatgg
      841 cctccacgcc cgatttcaac acctggatcg ggcgggcgat ccgtgccgcc acataccgcc
      901 ccatggccca gagcagacac agcatgacca gaccgatcaa cgcgagattc cgtaactggt
      961 cgcgaatcgg cgcgtaggtt tcctccggtt gttgccgtac gaagacgtgc caggagttat
     1021 ccggcaggct caaccccgtg accggcgcat accccaccac cgtatcggtc gcaccatggc
     1081 cgtcgtcctc ggcaattccc cagccgggat tcgacgagac gatcatggcc atcagttgac
     1141 tgggaatccg atgcgcgcgc cggggaagga taggacagac gagcggctga cccgcggcat
     1201 cgaacagcat ggcatggccg gtctgcccga tgcggatctc tttaatcatc gcgaaaaggg
     1261 aatcgaaccg gtaggacgcc ttgatggcac cgatgactgc atgctggtgg tcgtccagga
     1321 tcgggaccgc gatatcgatg gtttcttcag gcgagcggaa ggatccttcc tgtccgggaa
     1381 tcaacccgct gacgtagacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcctgca
     1441 aggcctccca ccaggattcc tggctgagga aataatggtc cggttccgag ctggctgcga
     1501 ccagcgcccc atagcgatcg gtgatcagca gccccaccat cttgtcaccg tcgcggactt
     1561 tggtctccag caggaagcga gacaattctg aattcagcag accggcggcg ctgtcatgcc
     1621 ctttctccca ctccttcgcg cgttcctgaa tcagccgctg cgaatcggcc cactcgcctt
     1681 tgtacgacag gttggcagcc tcgaccggct ggcgaacccg taagggcgcg gaggcgagca
     1741 accgcacccc ctgcacttcg ctctggacca gcatggtcag acgatcggcg gcctgttcgg
     1801 caatcgcctg gaaattgcca ccgatcacat cgcggaggga gcgcatgccg gacatcgacg
     1861 ccagcaccag gccgatcacc agcgggatac agcccaccag cacgatggcg agcacaatgc
     1921 ggcctttgat agtgcgaatc gtcacgccgg cctcctgcgt gacattctaa acaaccggcg
     1981 aaagaaaacg ccacagcacc ccgtcacggc accgatgggg tcaggcgtgg gtggtttcct
     2041 ccggtgccgt cggcacccgg gacgacaccg cggccgccag gccgtcgaca gccgctcgcc
     2101 gcgccctggc acgatgaaat ccccacaaga gcaggccgga tagactcatc agcagaatcg
     2161 gcacgcctgg aatattgagc agagtttttt cgaagccgaa aaaattcagc tggtgcaatt
     2221 gcatcacggc aaagtgaact tttcgccagc ggccctcatc ttccagaatc tcgccggtct
     2281 gtcggtccag cacaatctcc gtggcgccat cgtcatcgaa ccggacacgc gcggcatcgt
     2341 aggcgcgctt tttctttctg ggcgtgtacc gttcgatctc cacgcccacc accgtcgccg
     2401 ggctccgcac atactgcgcg gcaatcgtcg gggcaagctc cgacgtgatc ggcgacagcc
     2461 gttcaccgtt catggcatcc aacagcaaag ttgtcgagac cccttccacc cgtagcttca
     2521 cctcataaaa gagccggcca aagtcagtgc gcaaggtggc ctgctcgacc agcgcgcgac
     2581 ctcccaccgc ctctttcgcc aggaccagcg cccggtcgag cggcagtgtc gcggaatcga
     2641 ccggcggccc cgtgagcggt cgcacctttt ccttgtagtg ctcatcccaa tagagaaact
     2701 tcgcatccgc ccagagcagc cccgtcacga ctgacagcag gagaaaacca agcgccccta
     2761 tgccgagcca tcgatgcgcc cgcctgacaa tccgctcagc tcccccgcgg ctcatttact
     2821 tctcccctga gcgctcagcc cgcacctgaa tcgccagggt gctccgatgc cgaagcgcct
     2881 cgtatgtctt cccctgaaat tcacccggcg ttttttcgac gtgaatcaat tcaaggacat
     2941 accgtcccgg ccagagcggt gtgaaggcca caatcccttc cccatccgtt tttaactcct
     3001 tgtcccaccc aattggcgca tggaccaaga tcgtcgcatt cggaaggaac tgccccttga
     3061 agaaaccctt gacgacgatt tcaccgcccg gcacgtggct gactcgcccg gtcgccagat
     3121 tcagtccctt ggtcagggga atcatgtcga gggccatcgg cgtggcctga tcccgctcat
     3181 gctcgttgat gcggccctct tctaggcaca tgaaataggt ccgcgcataa aacatcggtt
     3241 tgacgatgcc gatgtcgtgt tttcgcaagt cctgaaccgg ggcctgcgcc tgctgcgcca
     3301 ccaccgcatt ccggccgggc agacacgacg gcagcgcccc ggcaaagtga ttgacctgtt
     3361 tggtcagagc tacttcagtc gtaccctgct tggagtcctg cacacgtaac gtgactccgt
     3421 cgatcgaatc cagacgccca ccgcgttcct ctcgtagaaa ctcgctgtat tcgccgaaat
     3481 agatcttgag cggctgaccg gtttcagccg gcgcgatggc gtcggcctcg acccacacga
     3541 aatgggccgt ggccggtgag ggccccacaa tcatagacag caacatcacg ctccacagtc
     3601 caactgcaca tcttctcctg ttcatcgtca acactcctta tatagagagt ccccggaggc
     3661 actgccccta ctcgttagaa tcgccatgac acgccgccgt aaaagttccg gatttctccg
     3721 ggctgaatgc cgataaagct gccggtcgtt cgttgcgcga taaacgattc gtcgctgagg
     3781 ttgcgcacgc cggcgaagac cgcccagcga gccttgggcg gcgcataatt gagcctgagg
     3841 ttccagatgg tgtaggacgg gatcgcgcct gtggtgccga gggcattttc cgtttgcgta
     3901 ttcacgccat cggtgaattg ctgggcgacg aacagggcat cggcctcgat cgaagcaccg
     3961 gtcgggtggt aatagcccac caatccgtag aacgtcatac gcggggcata aggaaggtct
     4021 ttcccgttcg tggcaccgaa aagcgacttc ggccgtaaga gggtgacatt tgccctggtg
     4081 accgacggga agagacgatc ggatcaggcc gttcgtttgg ctgccggt
//
LOCUS       DAORRQ010000152         4081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_638_length_4081_cov_7.342027, whole genome shotgun sequence.
ACCESSION   DAORRQ010000152 DAORRQ010000000
VERSION     DAORRQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_4081_cov_7.342027"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..403
                     /locus_tag="PK224_20840"
     CDS             125..403
                     /locus_tag="PK224_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18252.1"
                     /translation="MEEHDAEFRRSILDYLVHHPGAKDTQEGVLNWWITAPCLDEGAE
                     RLAIEALDKLVAQGWVLKRDTSNQPIYSLNHAHLKEIRTFLTQDGNET"
     gene            429..2033
                     /locus_tag="PK224_20845"
     CDS             429..2033
                     /locus_tag="PK224_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HRB18253.1"
                     /translation="MPVQPTYPGVYIEELPSGVRTITGVATSITGFLGKAVRGPVDQP
                     VTITSFSDYERIFGGLHRDMTLSYAVRDFFLNGGGQAVIVRLYKATAGSAAKATYEVP
                     NLTLQAASEGAWGMQVRARVEKKAVTDPNLIAVATRLGVAPADLFDLIVRDGSTGITE
                     TFLNLTTKESARRADRVLLAESNLVRVTTTMPSNSSPTAHAGTSADADVWTSTTKSTP
                     AKNAAPADIAVESAVLDAAAYKGSQTGKTGLYQLEKIDLFNLLCILPDARGGDVPDDV
                     YQEALSYCVKRRAMLIVDPKAAWATVGAAQSGAPGMNLNGDMARNAAIYFPRIKQADS
                     QLGGQVETFVPCGVIAGVMARTDSQRGVWKAPAGLDASLSGVAGLQLDMTDAENGLLN
                     PLGINCLRTFPVHGRVVWGSRTMRGADAAADEYKYVPVRRLALFLEESLYRGTQWVVF
                     EPNDEPLWAQIRLNLGAFMHNLFRQGAFQGTAPRDAYFVKCDKETTTQNDINLGIVNI
                     VVVFAPLKPAEFVIIKLQQMAGQIAT"
     assembly_gap    2227..2244
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2245..2758
                     /locus_tag="PK224_20850"
     CDS             <2245..2758
                     /locus_tag="PK224_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRB18254.1"
                     /translation="FTVNTHRFDPYKNFKFRVKWDGRYVAGVSKVGSLKRSTEVVEHR
                     EGGDPSTSRKSPGRTKYEAITLERGVTHDTAFEQWANKVWNFGSGLGAEVSLKDFRKD
                     IIIELYNEAGQLAIAYKVFRCWVSEYQAQSDCDANANAVAIQTLKLENEGWERDYEVT
                     EPSEPTFTEP"
     gene            2761..3501
                     /locus_tag="PK224_20855"
     CDS             2761..3501
                     /locus_tag="PK224_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18255.1"
                     /translation="MAHRSQSGLSAAAVIDIWEQGAGRHPLDQALLLLRYACPDEPFE
                     TLCEWTVGERDRRLLESRRNTFGDRIDGYAECPACCNGLEFELSCEGLLGSASMTDAT
                     WHKVEQAGRSWDVRGPNSRDLAAAAAAPDLDRARRVILSRCLRNGPDTVDESDWTDEL
                     QAALAARLSELDPLAEILIDLRCAACGHAWQSVFDIAGFFWNEIHVRGRRLLQEIDLL
                     ARTYGWTEGEILGMTDHRRRLYVGMALS"
     gene            3498..>4081
                     /locus_tag="PK224_20860"
     CDS             3498..>4081
                     /locus_tag="PK224_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18256.1"
                     /translation="MSDFLTRLVQRQAGTLATIQPRTRSMFAPAMDRPEPPTPDLSVI
                     DGVASSGEQRQPAEAPRHSIAPMPPLAAFLHAQRRIEAPVDEPSVVLPETGARGTEPS
                     NESPVVKVTAVPSAAPGEGPPVSLRNQIVESDRHAARVFSRDNARPEERRSEATAAQE
                     RAITPPPRLVEAQQATPMSARVTPPSLVSTGQQGR"
BASE COUNT          812 a         1168 c         1334 g          749 t
ORIGIN      
        1 ccctccgatt acacctgttc gattcccttt aaaacgcagg aaacgggatt ctccctaatg
       61 gcattgctct tgctcactcc cgcaggagtg ccgtgatgga gcggggctcc ggggatcagg
      121 cctgatggaa gagcacgatg cagagttcag gcgcagcatt ctcgactacc tggttcatca
      181 tcctggcgcg aaagacactc aggaaggggt gctgaattgg tggatcaccg caccgtgcct
      241 ggatgaaggt gctgaacgtc tcgcgatcga ggcgttggac aagctggttg cgcaaggttg
      301 ggtcctgaaa cgcgacacgt cgaatcagcc gatctatagc ctgaatcatg cgcacttgaa
      361 ggagatacga acatttctga cacaggacgg aaacgagacg tgaacgtcct aagccgagga
      421 ggatgagcat gccagtgcaa ccaacctatc cgggtgtgta catcgaggag ctgccgagcg
      481 gggtgcgcac gatcaccgga gtcgccacgt cgatcacggg gtttctggga aaggccgtgc
      541 gcggcccggt cgatcaaccg gtgacgatca cgagtttttc ggattacgag cggatcttcg
      601 gcggactgca tcgcgatatg accttgagct acgccgtgcg tgactttttt ctgaacggcg
      661 gtggacaagc cgtgatcgtg cgtctctata aggcaaccgc ggggagtgcc gcgaaggcga
      721 cctatgaagt gccgaacctg acgttgcagg ctgcatcgga aggcgcgtgg ggcatgcagg
      781 ttcgcgcgcg ggtcgagaaa aaggctgtga ccgatccgaa tctgatcgcc gtcgccacca
      841 ggctcggcgt ggcacccgcc gatctgttcg acttgattgt ccgcgacggg agcaccggta
      901 tcacggaaac attcctgaat ctcacgacga aggaaagcgc gcgtcgcgcg gaccgtgtgc
      961 tcctggcgga atcgaatctg gtccgtgtta cgacgacgat gccgtcgaat agctcgccca
     1021 cggcccatgc cgggacttcg gcggatgccg acgtctggac ttccaccacg aaatccacgc
     1081 ctgcgaagaa tgcggctccg gcagacattg ccgtcgagag cgccgtgctc gatgcagcgg
     1141 cctacaaagg aagccaaacc ggtaaaaccg gactctacca gctggaaaaa atagatctct
     1201 tcaacctgct ctgtatcctg cccgacgcgc gcggcgggga tgtgccggat gacgtgtatc
     1261 aggaggcgct cagctactgt gtcaagcggc gggccatgct gatcgtcgat ccgaaggcgg
     1321 cctgggcgac ggtgggcgct gcacagtcgg gggcacccgg gatgaacctg aacggcgata
     1381 tggcgcgcaa cgcggcgatc tacttcccgc gcatcaagca ggccgattca cagctcgggg
     1441 gacaagtaga gaccttcgtg ccctgtggcg tgattgccgg tgtgatggcg agaacggaca
     1501 gccaacgcgg agtgtggaag gcgccggcgg ggctcgacgc ctcgttgagc ggcgtcgccg
     1561 ggctgcagct cgatatgacg gatgcggaga acggactctt gaatccgctg ggtatcaatt
     1621 gtctccggac attcccggtc cacggtcgag tggtatgggg ttcccggacc atgcggggcg
     1681 ccgatgcggc ggcggatgaa tacaagtatg tgccggttcg tcggctggcc ctgttcctcg
     1741 aagaaagtct ctatcgcggg acgcagtggg tggtgttcga accgaacgat gagcccctct
     1801 gggcgcagat caggctgaac ctgggcgcct tcatgcacaa cctgtttcgg cagggtgcat
     1861 ttcaaggcac cgcaccgcgg gacgcctact tcgtgaagtg cgacaaagag accacgaccc
     1921 agaacgatat caacctcggc atcgtcaaca tcgtcgtcgt attcgccccg ctcaagcctg
     1981 cggagttcgt catcatcaag ctccagcaga tggccggcca gatcgcgacc tagcggcttg
     2041 tggaacagcc gggtgtcggg gccggatgag tgtatgggtg gatagggaaa cgattttatc
     2101 accactcgca ggatgctcaa aaaggccgtc cgccgcggcc gcagcgagcg caggggcgag
     2161 gcgtactatt cccgtacgtc gagcttctgt gcgatgcgag aacaaaggtg gctgattttt
     2221 tcagcannnn nnnnnnnnnn nnnngtttac cgtcaacacg catcggttcg atccctacaa
     2281 gaatttcaaa ttccgggtca aatgggacgg gcgttacgtc gcgggggtca gcaaggttgg
     2341 ttcgttgaag cgctccaccg aagtggtcga gcaccgcgaa gggggcgatc caagcaccag
     2401 ccgtaaatcg cccggtcgta ccaagtatga agcgatcacg ctggagcggg gtgtcactca
     2461 cgataccgcc ttcgagcaat gggcgaacaa ggtctggaac ttcgggtccg ggttgggcgc
     2521 ggaagtatcg ctcaaggact tccggaagga catcatcatc gagttgtaca acgaggcggg
     2581 acaactggcc atcgcctaca aggtcttccg ttgttgggtg tcggagtatc aagctcagtc
     2641 tgactgcgat gccaatgcga atgcggttgc gatccagacc ctgaagctcg agaacgaagg
     2701 ctgggaacgc gactacgaag tcacagaacc gtcggagccg acctttacgg agccgtgacg
     2761 atggcgcaca gatcccagtc aggactctcc gcagcggcgg tcatcgacat ctgggagcag
     2821 ggcgccggcc gacatcccct cgaccaggcg ttgttgctgc tgcggtatgc ctgtcccgac
     2881 gagccgttcg aaaccctgtg cgagtggaca gtgggggagc gggataggcg gttgttggag
     2941 tcccgtcgaa acaccttcgg cgatcgcatc gatgggtatg cggaatgtcc tgcctgctgc
     3001 aacggactcg aatttgaact gtcctgcgag ggtttgctgg gatcggcttc gatgacggac
     3061 gcgacctggc acaaggtgga gcaagcgggg cgctcgtggg atgtgcgcgg gccgaacagc
     3121 cgggatcttg ccgccgccgc cgccgctccg gacttagatc gggcgcgccg tgtgattctc
     3181 tcgcgttgcc tgcgcaacgg acctgacacg gtggacgaat ctgattggac ggatgagttg
     3241 caggcggccc tggcggcgcg actgagcgag ctcgatccgt tggccgagat cctcatcgac
     3301 ttgaggtgcg ctgcctgcgg gcacgcatgg cagagcgtct tcgatatcgc gggatttttc
     3361 tggaacgaga tccatgtgcg aggccggcgg ttgctgcagg aaattgatct gctggcgcgc
     3421 acctatggct ggacggaagg cgagatcctc ggcatgaccg atcaccggcg ccgcctgtat
     3481 gtggggatgg cactctcatg agcgattttc tcaccagact cgtccaacga caggccggaa
     3541 cgctcgctac gattcaaccg cgcacccgtt ccatgtttgc ccctgcgatg gatcggccgg
     3601 agccgccgac gcccgatctt tcggtgatcg acggggtggc ctcgtcgggt gagcagcgcc
     3661 aaccggccga ggcgccgcgt cattcgattg ctccgatgcc tccattggcg gccttcctcc
     3721 atgcgcaacg gcgcatcgaa gctcctgtcg atgaaccgtc tgtcgttctg ccggaaacag
     3781 gggcgcgcgg gacggagccg tcgaacgaat cgcccgtcgt caaagtaacg gccgtcccaa
     3841 gcgcagcccc gggagaaggg ccgccggtat ccctgcgcaa tcagatcgtc gaatcggacc
     3901 ggcatgcagc tcgtgtgttc tcccgcgaca atgcaaggcc ggaggaacgg cgcagtgagg
     3961 caaccgctgc gcaagagaga gcgatcacgc ctccgccacg gttagtcgag gcgcaacagg
     4021 cgacgccgat gtcggcccgc gtcactccgc cttcgttggt gtccacggga cagcaagggc
     4081 g
//
LOCUS       DAORRQ010000153         4009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_649_length_4009_cov_5.693981, whole genome shotgun sequence.
ACCESSION   DAORRQ010000153 DAORRQ010000000
VERSION     DAORRQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_649_length_4009_cov_5.693981"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PK224_20865"
     CDS             <1..814
                     /locus_tag="PK224_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRB18257.1"
                     /translation="FKVKKGKTERYLKDEPAMNEYLADLAVEEVEVALENGREYLSGR
                     RLLPTLKKLIAFESLLHKVNKKHHEANMLRIFVDEPGLTREALKDQAALTTIVANAKA
                     TLTLVYPKAEPTVDILEDEEHQSSKLVCKVATGGIAYQLDVTHELVGSADFRELQKQA
                     PSAMGLGKPPYKIKIKGTEVHKNGSAELVQAILEEGKQGLNIQRYKGLGEMNPGQLWE
                     TTMDPEKRTLLRVKLEDMTGVDEIFTILMGDEVEPRRNFIQQHALEVRNLDV"
     gene            992..>1912
                     /locus_tag="PK224_20870"
     CDS             992..>1912
                     /locus_tag="PK224_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRB18258.1"
                     /translation="MPPDERLGQIAIEDEMRSSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRILHGMNEMGLAANRPYRKSAKIVGEIMGNYHLHGDSAIYDTLVRMAQNFNMRYMLV
                     DGQGNYGSMDGDAAAAMRYTEARLTKLAEELLADIEKETVDFGPNYDESRVEPLVLPS
                     RVPNLLVNGAGGIAVGYATNIPTHNLGEVIEGLLLLLENPDVTIPQLMKKIPGPDFPT
                     AGFIYGTSGIKDAYETGRGLLTVRAKVAIETDERTDRERLIVTEIPYQVNKSKLIEKI
                     ADLAQEDRVTGISDIRDESDREGVRVVIELK"
     assembly_gap    1913..1968
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1969..>2258
                     /locus_tag="PK224_20875"
     CDS             <1969..>2258
                     /locus_tag="PK224_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRB18259.1"
                     /translation="LYKHSQLQTTFGVNMLALVNNRPEVLNLKRILEAFVEHRREVVV
                     RRTAYDLRKAEERAHILEGLKIALDNLDAVIALIRRSQSPDVARTGLMQQFR"
     assembly_gap    2259..2268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2269..2793
                     /locus_tag="PK224_20880"
     CDS             <2269..2793
                     /locus_tag="PK224_20880"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRB18260.1"
                     /translation="PFAARLSEIQANAILEMRLQRLTQLERDKLVEEYREVLKTIEYL
                     RSVLGSEALVRKIIQDELIEIKDKYQDERRTKIVKEEAELTLEDLIAEEEVVVTISHA
                     GYIKRNAVTLYRAQRRGGKGKIAMGIKRISSKRSLRPRRMTHCSSSPMPARSIGSKCT
                     KFRKPAGPRRARHL"
     gene            2742..>2877
                     /locus_tag="PK224_20885"
     CDS             2742..>2877
                     /locus_tag="PK224_20885"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18261.1"
                     /translation="MHEIPEASRAAKGKALVNLLALSKDEKVTASLPVKEFRADRFII
                     M"
     assembly_gap    2878..2911
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2912..3496
                     /locus_tag="PK224_20890"
     CDS             <2912..3496
                     /locus_tag="PK224_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRB18262.1"
                     /translation="AYSNPRQGGIIALSLDAGDKLIGVDLTDGQREILLGTKQGITIR
                     FKEEDVRAMGRTAHGVRGITLEPGDEVIGMETITPDSTTAILTVTEGGYGKRTPVNEY
                     RVQGRGGKGIISVKTTERNGPAVGFLQVRDEDEIMLMAAQGKVLRCKVDDIREIGRNT
                     QGVRLLDMDGDEDRVVAVVRLVEREEGVPEEGES"
     gene            3543..>4009
                     /locus_tag="PK224_20895"
     CDS             3543..>4009
                     /locus_tag="PK224_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18263.1"
                     /translation="MNHPTPPSAGAPTRTPAKRPLDTVVKMALGVLFGSFALIWGGMF
                     LSRPDRSIPPYSIGSQERTVVAVHVPSWTSDTEIETLIERFRKVGAESRNFGSMKIQP
                     TTLDDPKGRYQHIAIYIFTDEGWTEPEVLHKYVTGDDATVRDGFEGSVRGLYRL"
BASE COUNT          886 a         1091 c         1247 g          685 t
ORIGIN      
        1 ctttaaggtc aagaagggca agacggagcg gtatctcaag gatgagcctg cgatgaacga
       61 atacctcgcg gacttggcgg ttgaagaagt cgaagtggcg ctcgagaacg ggcgggagta
      121 cctgtcgggc cgccgcctgc tgccgacgct caagaagttg atcgccttcg aaagcctgct
      181 gcacaaggtg aataagaaac atcacgaagc caatatgttg cggatatttg tcgacgagcc
      241 ggggttaacg cgcgaggcgt tgaaggatca ggccgcgttg accacgatcg tcgccaatgc
      301 gaaggccacg ctgacgctcg tctacccgaa ggccgaaccg accgtcgaca tcctggaaga
      361 cgaagaacat caatccagca agttggtatg caaggtcgcc acgggcggga tcgcgtatca
      421 actcgacgtg acgcatgagc tggtcgggtc cgccgacttc cgcgagctcc agaaacaggc
      481 gccctcggct atgggcctcg gcaagccgcc gtacaagatc aagattaagg gaaccgaagt
      541 ccataagaac ggctcggccg agctggtgca agcgatcctg gaagaaggca agcaggggtt
      601 gaacatccag cggtacaaag gtctcggtga gatgaatccc ggccagttgt gggaaaccac
      661 catggatcct gaaaagcgga cgctgctgcg ggtcaagctg gaagacatga ccggcgtgga
      721 tgagattttc acgatcctga tgggagacga agtggaaccc cgccgcaatt tcattcagca
      781 acatgcactg gaagtcagga acttggacgt atagctggtc ctgattgcga agacgacaac
      841 ttggttttgt gaattcggag gatgctcaaa atggcgcttc ggcaaggccg caggcgagag
      901 aggaccggag gcgtaccctc aggggtacgt tgaggacctt cttgagccga gaacgaagcc
      961 gagggacatt ttcagcatcc gtggagggta gatgccgcca gatgaacgac taggacagat
     1021 tgcgatcgaa gacgagatgc gctcgtcgta cctcgactac gccatgagcg tgatcgtcgg
     1081 tcgcgcgctc cccgacgtgc gggacggatt gaagccggtg caccggcgca ttctgcacgg
     1141 catgaacgaa atgggtctgg ccgcgaaccg gccgtacaga aagtcggcca agatcgtggg
     1201 cgagatcatg ggtaactacc acctccatgg cgattcggcg atttacgaca ccctggtgcg
     1261 catggcgcag aacttcaata tgcgctacat gctcgtcgac ggacagggca actacggctc
     1321 gatggacggc gacgccgccg ccgccatgcg gtacaccgaa gcccggttga ccaagctggc
     1381 cgaagagctg ctggcggata tcgaaaaaga gacggtcgat ttcggcccca actacgatga
     1441 atcccgtgtc gagccgttgg tcctgccgtc ccgagtgccg aatctgctgg tcaacggcgc
     1501 cggtggaatc gccgtcgggt atgcgaccaa cattcccacg cacaatctgg gtgaagtgat
     1561 cgagggactg ctcctgttgc tggagaaccc cgacgtcacc atcccgcagc tgatgaagaa
     1621 gattcccggg cccgacttcc cgaccgccgg gttcatttac ggcacctccg ggatcaagga
     1681 tgcgtacgag acgggacgcg ggctgttgac ggttcgcgcc aaggtcgcca tcgagacgga
     1741 cgagcgcacc gaccgtgaac ggctcatcgt gaccgaaatt ccgtaccagg tgaacaagtc
     1801 gaaactcatc gaaaagatcg ccgacctggc gcaggaggat cgcgtcacgg gcatctccga
     1861 tatccgcgac gaatcggacc gtgagggtgt gcgtgtcgtg atcgagctga agnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ctgtataaac
     1981 acagccagtt gcagactacc ttcggcgtca acatgctggc gcttgtcaac aaccggccgg
     2041 aggtgctgaa tctcaagcgc atcctggaag cctttgtcga gcaccggcgc gaagtggtgg
     2101 tgcggcgaac ggcctatgac ttgcgtaagg cggaagaacg cgcccatatc ctcgaaggtc
     2161 tcaagatcgc gctggataat ctggatgccg tgatcgcgct gatccgccgc tcgcaatcgc
     2221 cggatgtggc gcgcacgggt ttgatgcagc agttccggnn nnnnnnnncc cttcgcggcc
     2281 cggctgtcgg aaatccaagc caacgccatt ctcgaaatgc gcctccagcg gctcacgcaa
     2341 ctggagcgcg acaaactcgt ggaagaatac cgcgaagtgc tgaagaccat cgagtatctg
     2401 cgctcggtcc tcggcagcga ggccctggtg cggaagatca tccaggacga actgatcgag
     2461 atcaaggaca agtatcagga cgaacggcgc accaagatcg tcaaggaaga ggccgagctg
     2521 acgctcgaag atctcatcgc cgaagaagaa gtggtcgtga cgatttccca tgccgggtat
     2581 attaagcgga acgccgtcac gctctatcgg gcgcagcggc gcggcggcaa gggcaagatc
     2641 gcgatgggca tcaagaggat ttcgtcgaaa cgctctttac ggcctcgacg catgactcat
     2701 tgctcttctt caccgatgcc ggcaaggtct attggctcaa agtgcacgaa attccggaag
     2761 ccagccgggc cgcgaagggc aaggcacttg tgaacctcct ggccctctcg aaggatgaga
     2821 aggtcacggc ttcgctgccg gtgaaagagt tccgtgccga tcgctttatc atcatggnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcctacagc aatccgcgcc agggcggcat
     2941 cattgcgctc tcgctcgatg cgggcgacaa gctgatcggt gtggacctga ccgacggcca
     3001 gcgtgagatt ctgttgggca cgaagcaggg catcaccatc cggttcaagg aagaagatgt
     3061 gcgcgcgatg ggacgtacgg cccacggggt gcgcggaatc acccttgagc ccggcgatga
     3121 agtcatcggc atggagacga tcacgcccga ttcgactacc gcgatcctca ccgtgaccga
     3181 aggcgggtac ggcaaacgga caccggtgaa tgaatatcgc gtgcagggcc gcggcggtaa
     3241 aggcatcatc agcgtcaaga cgaccgagcg aaacgggccg gctgtcggat tcctccaggt
     3301 ccgtgacgag gatgagatca tgttgatggc ggcgcagggc aaagtgctgc gctgcaaggt
     3361 ggacgacatc cgtgaaatcg ggcgcaacac ccaaggtgtc cgcctgctcg atatggatgg
     3421 agatgaggat cgtgtggtcg ccgtcgtgag actggtcgaa cgcgaagaag gagtgcccga
     3481 agagggcgag tcatgactcc ttggagccgc accggtttca agcggctccc cccaccatgg
     3541 ttatgaacca tccaactccg ccatcggccg gggctccgac cagaactccg gccaagcgtc
     3601 cgctggacac cgtggtcaaa atggcgctgg gcgtcctgtt cggctccttt gcgttgatct
     3661 ggggcgggat gtttctcagc cgtccggatc ggtcgattcc cccctatagc atcggctccc
     3721 aggagcgcac ggtggtagcg gtgcatgtgc cgtcctggac cagcgacact gagatcgaaa
     3781 ccctgatcga gcgattccgg aaagtcggcg cggaaagccg gaacttcggc agcatgaaaa
     3841 tccagcccac caccctggat gatcccaagg ggcgttatca gcatattgcg atttacatct
     3901 tcaccgatga gggctggacg gagccggagg tgctgcataa gtacgtgacg ggtgacgatg
     3961 ccacggtgcg ggacgggttt gagggatcgg tacgcggatt gtaccggct
//
LOCUS       DAORRQ010000154         4008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_650_length_4008_cov_5.488743, whole genome shotgun sequence.
ACCESSION   DAORRQ010000154 DAORRQ010000000
VERSION     DAORRQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_650_length_4008_cov_5.488743"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PK224_20900"
     CDS             complement(<1..1668)
                     /locus_tag="PK224_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18264.1"
                     /translation="MANLTATKRWEVEQTLTAMPKSRRGEATPAPLTLDVEGAPDDAY
                     IVMTRYNSGAIRFHMPTEPARRGIRRGGKRIYRFSIPVPAAPIAEAGGRRGFISAAIK
                     AVVLKVAGKIADFALSKLALLWETATWKLKKQREGWLSITADSLGGESGLPAADLQTL
                     SASERNLLFIHGTFSSTKAAFRSLAQTPGSNGRTFFEELQAVYGNRIYGFDHFTLSRT
                     PQENVQMLLEALPARPTTFDVITHSRGGLVLRHVVERRDLFGSLADRFVLGRAVLVAS
                     PNEGTPLASPDHVTQYTTWLSNVLELFPDNPFTTGVEFVSEALSWIARRLVGGLPGLA
                     SMDNKGDIIQALQATPNPPNETYSALVANYEPDAGVLQRIIDAGADLFFPTANDLVVP
                     TEGGWRVDVGSGGTVTIPGTRVGCFGLGGNLTQQKAVNHVNFFDDPGTVDFLVRALRG
                     EAQPAAPIDIEHDLPSGKRRGAVRTEARPTIAAGPALQQPATISPTVAATPASKAVPP
                     IVSLTQSPEPDDVFQLALIDPDPRDAVATLIATFRNARVMETVHIGTG"
     gene            complement(2361..3536)
                     /locus_tag="PK224_20905"
     CDS             complement(2361..3536)
                     /locus_tag="PK224_20905"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRB18265.1"
                     /translation="MTRADKRTTSCSPNLDFLRASAVLIVYFFHLFITTNTRLPDYLG
                     QFGVLLFFVHTSLVLMFSLERIEATEGSFFLTFYLRRLFRIYPLSIFCVSVIVLFQLP
                     RAPWWPWSNPDLSTILANLFLYTEFMYKPVVTSVLWSLPYEVAMYMVLPFLYLAGKAY
                     GVRGIFVLWCVAVVGAIAQPYVSSRLGVAQYAPCFIAGVASYFLGFGMQRFRLPFIGW
                     PLTLLIAGAILGYGNHSEHEITAQWALCVLIGVTAPFFAELQLPAMRKACALIARYSY
                     GIYLTHLHAQWTAFVLLKDSPSAVRYSVLVVLSVGLPVALYHLIEAPMVRMGASLAQR
                     LPRTSAQSSKPTPAQRPLPVSATAAPHTPLHAAARPRAAHAPDHRHSKTAPARVSAS"
BASE COUNT          831 a         1082 c         1317 g          778 t
ORIGIN      
        1 gcccgtcccg atgtgcacgg tttccatcac ccgcgcattg cggaaggtgg cgatgagcgt
       61 cgcgaccgca tcgcgcgggt cgggatcgat gagggcgagt tggaacacgt cgtcaggctc
      121 ggggctctgc gtcagggata caatgggcgg gactgccttg gaggcaggag tcgcagccac
      181 tgtcggggag atcgtcgccg gttgctgaag cgcaggaccg gccgcgatgg tgggccgcgc
      241 ttcggtgcga acggcgcccc gccgcttgcc ggatggcagg tcgtgctcga tgtcgatcgg
      301 tgcggcaggc tgggcctccc cgcgcagggc gcgcaccaaa aagtctaccg tgcccggatc
      361 gtcgaagaag ttcacatggt tcaccgcctt ctgctgcgtc aggtttccac ctaatccaaa
      421 gcagccgacg cgagtgcccg gaatcgtcac cgtcccaccg cttccgacgt ccacccgcca
      481 cccgccttcg gtcggcacga ccagatcgtt ggccgtcgga aagaataagt cggctcccgc
      541 atcgatgatt cgctgcagca cccccgcgtc cggttcgtaa ttggcgacca gagccgagta
      601 ggtctcgttg ggagggtttg gggtggcctg caacgcctgg atgatgtcac ccttattgtc
      661 catcgaggcc aatcccggta gcccgcccac cagacggcgg gcgatccagg agagggcttc
      721 gctgacaaat tctacgccgg tggtgaaggg attgtcggga aacagttcaa ggacgttgga
      781 gagccaggtt gtgtattggg tcacatgatc gggtgaggcg agcggggtgc cttcgttcgg
      841 gctggcgacg agcacggccc ggcccagtac aaaccggtca gccagcgagc cgaagaggtc
      901 gcgacgttcc accacatgcc gcaggaccag gccaccgcgc gaatgggtga tcacgtcgaa
      961 ggtcgtcggg cgagcgggga gggcttcgag caacatttgc acgttttcct gcggcgtgcg
     1021 actcagggtg aagtgatcaa atccatagat gcgattgcca tacacagcct gcagttcttc
     1081 aaaaaatgtc ctgccgttgc tgcccggggt ctgcgccagc gagcggaagg ctgctttggt
     1141 gctggaaaag gtcccgtgga tgaacagaag attccgctcc gatgcgctca acgtctgcaa
     1201 gtctgcggca ggcaggcctg attctccgcc gagactgtcg gcggttatgg acagccaccc
     1261 ttctcgttgt tttttcaatt tccacgtggc ggtttcccac agcagcgcga gtttcgagag
     1321 cgcaaagtcg gcaattttcc ctgccacttt gagcacgacg gccttgatcg ccgcgctgat
     1381 gaatccgcgg cggccgcctg cttcggcgat gggcgcggcc ggcaccggga tcgagaaccg
     1441 atagattctc ttcccgcctc gtcgaatgcc gcgtcgagcc ggttccgtcg gcatgtggaa
     1501 gcggatggct ccggagttgt atcgcgtcat cacgatgtac gcgtcgtcag gagcgccttc
     1561 gacatccagg gtgaggggag ccggagtcgc ctcgcctcgt ctggacttcg gcatcgcggt
     1621 gagggtttgc tcgacttccc agcgcttggt ggcggtgagg ttggccacga aggggtcagg
     1681 tacctcgccc gctgctcgac gccctccacg agcctgttcc gtagcacgaa cgtcccagcc
     1741 ctctgttgtc gaccagctca ctgtgcctga ttttttgtgc gccatggtgt gcccccactt
     1801 cgctcgtcag accgagcgca atcgattcat gtgatcctgt agctcgtgtc tcgtgaagcg
     1861 tgaggtgagc gaagttgaaa cgagatacgc ttcacgagtg acgagcggcg gcggttcgac
     1921 ttgtggccgt cacgaactgc ttgcgcttat cccataaccg gaaaaatgag gcaagagatt
     1981 cgttggggcg cagggagaaa tgcaagtatt acgctgcccg aaacgaggct gacagcagag
     2041 cagggggaac tgggatgtga acgggagaat gctgagaagt cgggtgttgt ttccagatca
     2101 gcctgcttgc tcgttggcca actttgcgat acatgcgcac gatgcgctcg attggagagg
     2161 aacgacgatg atggggagaa tggtttatgg ggtcattcag gcagctgtcc caaatagata
     2221 gtctccattc ttgccctatc ttgccctgga acgttgaatc gagcgaggaa aaggcgtggg
     2281 gcagcgatga atcggtttgc cgtcatctgt tgcaggctca tacggtcgat gcgccgaagc
     2341 gttcggctgc gagaaaccgg tcaacttgcg gagacacgag caggtgcggt tttggagtga
     2401 cgatgatcgg gcgcgtgggc tgcgcgcggc cgcgctgccg cgtgtagggg tgtgtgcggg
     2461 gcggcggttg ctgagacggg caatggccgt tgagcgggag tcggcttgga ggattgtgcg
     2521 gaagttcttg gtaaccgctg ggccaagctg gctcccatcc tgaccatcgg ggcttcgatc
     2581 aggtgataga gggcaactgg cagaccgacg ctcaggacga cgagcaccga atagcgtacc
     2641 gccgaggggg agtccttcag caagacaaac gcggtccact gggcatgtaa atgcgtgagg
     2701 tagatgccat acgagtatcg cgcaatcagg gcgcaggcct tgcgcattgc cggtagctgg
     2761 agctcggcaa agaacggcgc ggtcacacca atcagcacgc acagggccca ctgggcggtg
     2821 atctcatgtt cggagtgatt tccatacccc aagatagcgc cggctatgag cagagtgaga
     2881 ggccaaccga taaacgggag ccggaatcgt tgcatcccga accccaggaa gtagctggcc
     2941 acgccggcga tgaagcaggg cgcatattgg gccaccccga ggcggcttga cacgtaaggc
     3001 tgggcaatcg caccaacgac agcgacgcac cagagtacga aaattccacg gacgccatag
     3061 gccttcccgg cgagatagag gaacggcagc accatgtaca tggccacctc ataggggaga
     3121 ctccagagga cggaggtgac cactggtttg tacatgaact ccgtatagag gaacagattg
     3181 gccaggatgg tcgacaggtc cgggttggac cagggccacc agggagcgcg gggcagttga
     3241 aagaggacga tcacggaaac acagaagata ctgaggggat agatgcgaaa cagccggcgg
     3301 agataaaagg tcagaaagaa gctgccctct gttgcttcga tccgctccag cgagaacatc
     3361 aagacgagac tggtatggac gaagaagagc agcacgccaa actggccgag ataatcaggc
     3421 aaccgggtgt tcgtcgtgat gaagagatga aaaaaataga cgattaaaac cgcactcgcg
     3481 cggaggaaat cgagattcgg cgagcaggac gttgtccgct tatcagcgcg ggtcatccaa
     3541 cgacgtcctc ctcgtgaaac cgctctgttg ttgtggtgcg atcgtattcg tcacttcaat
     3601 taccgaggcc agagcaggaa ttgggcagtt cccaccctgc gctgatgcgc gggacgtaca
     3661 tgtcggaatt ggttgaaccg actgtcagga tggccaatgt cccggttgtg agtgaatcgg
     3721 gaattggtgc aggctgagag ctctggttgt tccggtcact cgggatccta tggtgtagga
     3781 attcgtgacc agaacggagt acacattatt caccttccag ctgtcaaccc tccgggagca
     3841 gttttcttgg acgctgcatt cgatgttcca ttttaggtca tgcccgtgat gtgcatcagc
     3901 tgaactaatc tgtcttatac aggagagaga caagaggggg tgctaaggag cgttgcgtat
     3961 cagctcccgc ttgaaggtca accggtcagt gtcggagaga cccggtgg
//
LOCUS       DAORRQ010000155         3979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_655_length_3979_cov_34.305046, whole genome shotgun sequence.
ACCESSION   DAORRQ010000155 DAORRQ010000000
VERSION     DAORRQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_655_length_3979_cov_34.305046"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK224_20910"
     CDS             <1..198
                     /locus_tag="PK224_20910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRB18266.1"
                     /translation="GEPATFPLNQVIKCWTEAVQLIKVGGKSKLVCPSGIAYGDRGSP
                     PVIKPGATLIFEVELLDIVKQ"
     gene            392..1315
                     /locus_tag="PK224_20915"
     CDS             392..1315
                     /locus_tag="PK224_20915"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB18267.1"
                     /translation="MLFPQTQLLLVDDSPLNLNILLEFLREKEYSCTTAQSGVQAWTM
                     LEREPDRFHAVLLDRIMPEMDGMEVLHRMKHHTVLSQIPVIMQTAASTHQQTLEGLQA
                     GAYYYLAKPFDKATLQAIVDAAVRDHISYLDVRQDLRRTTTTMRLLNSGTFRFKTPEE
                     AKNLAMLLAHAYPDANRVVTGILELTLNAIEHGNLNIGYKEKSRLIEEERLDNEIERR
                     LSDPLYSSREATAQFVRRPDRLSLHITDQGKGFEWRKYLNFDPERAFDTHGRGIAMAN
                     KVSFDRIEYRGNGNQVITMLQLGTVAPALVA"
     gene            complement(1391..2791)
                     /locus_tag="PK224_20920"
     CDS             complement(1391..2791)
                     /locus_tag="PK224_20920"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HRB18268.1"
                     /translation="MRRRGWRAATLVMLLLGTAGCADSLSGMSGAGDVKGTGRTVTVQ
                     PREIDEVLDNPGMGFADFHFGFGHPPAPEEYPHTTVAYFRWPWAELEPKEGQYNFALV
                     DRVIEQAKAKGETLAIRIVSEYKTGSPQWLLDKGVGSVKESDGIFPDYNHPVFLDYHE
                     RLIRAFGERYGRSVDIDHVDIGSIGCWGEWNTVCCEGVEAQCKAFFPTEANQIAITDW
                     YLKYFAGTPLVMLHGGQLKYAASHGAGWRGDCFGDYGYFSPDWNHMEHAYPPVLEEAV
                     IANAWKRGPVQMEVCGYIHEWYDRGFDLDRILNQGLEWHLSVLNAKSKPVPAAWRPRF
                     NEFLKRIGYRFVLRELTHSAESHPGGPLVLQSRWENKGVAPIYHAWPLAYRLRSSSDQ
                     VVAQWTSPADLKQWLPGPSPRVEDTVVVPETLSAGSYALDVAILSEDALSAHVELAIE
                     GKRADRWYALSRVEIR"
     gene            3095..3979
                     /locus_tag="PK224_20925"
     CDS             3095..3979
                     /locus_tag="PK224_20925"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRB18269.1"
                     /translation="MLNWFSQSPQSDSVVITGASTGIGAACALALDKLGYRVFAGVRH
                     PADGERLQQQAGPRLMPIRLDVTDAASISAASHTVAAMVGERGLAGLVNNAGIGVAGP
                     IELLPLADWRRQFEVNVFGLIAVTQTFLPLIRTGRGRIINMGSIAGRASMPFMAPYAA
                     SKHALEAITDALRLELQPWGIRVALIAPGAIATPIWKKTRKEVDAWDAAWGQDLKGMY
                     REGFTRIKDAATAAGEQAQPASVVAETVAHALRSRWPKTRYLVGSDAAIRAYLALLLP
                     DRLNDWLLTRIVKLPPRR"
BASE COUNT          848 a         1328 c         1086 g          717 t
ORIGIN      
        1 ggcgaaccgg ccaccttccc gctgaaccag gtcatcaaat gctggaccga agcagtacag
       61 ctgatcaagg tcggcggcaa gagcaaactg gtctgcccgt cgggcatcgc ctacggggac
      121 cgtggatcac ccccggtcat caagccgggt gccaccctga ttttcgaagt ggaattgctg
      181 gacatcgtca agcagtagcc cccttccgga aggactctac cggggcacat cccccggcgg
      241 agtccttcgg ttttcctctc tttcagccct cacttaagta ttctccgtcg acttccgata
      301 gggctatcag tgccttgtat tccgatgcga ggcccacgac ttctgcccta gaccccgacg
      361 gccaagccgg caaccggagg tgccacatat catgctgttc ccgcaaacac agttattgct
      421 cgtggatgat tctccactca acctcaatat cctgctcgaa ttcctacgcg agaaggaata
      481 ttcgtgcacc acggcacaaa gcggcgtcca agcctggacc atgctggaac gagaaccgga
      541 ccgattccac gccgtcctcc tggaccgtat catgcccgag atggacggaa tggaagtcct
      601 ccacaggatg aagcatcata ccgtcttgag ccagattccc gtgatcatgc agacggcggc
      661 ctccacacat cagcagaccc tggaaggact ccaggcagga gcctactatt acctggcaaa
      721 gccgttcgac aaagcgaccc tgcaagccat cgtcgacgcg gcagtccgcg atcacatcag
      781 ctacctggac gtacgacagg acctgcgccg gaccaccacg accatgcgcc tgctgaactc
      841 gggaacgttc cggttcaaaa cgcctgaaga ggcgaaaaac ctggcgatgc tactcgcgca
      901 tgcctatccc gatgccaatc gggtggtcac cggcatcctc gaactcacct tgaacgcgat
      961 cgaacacggc aacttgaata tcggctataa agagaaatca cgcctcatcg aagaggaacg
     1021 gctggacaat gaaatcgagc gacggctgag cgatcccctc tactcgtccc gcgaggccac
     1081 ggcacaattt gtccgccgtc cagacagact ctccctgcac attaccgatc agggcaaggg
     1141 attcgaatgg cggaagtatt tgaacttcga ccccgagcga gccttcgaca cccatggccg
     1201 agggatcgcc atggccaaca aagtcagctt cgatcggatc gagtatcgcg ggaacggcaa
     1261 ccaggtgatc acgatgcttc agcttgggac tgtcgcgccg gcactggtcg cgtaacaacg
     1321 cctcccacca caacgatccg cctctcataa caccgacacc ggccgaacag acctacacca
     1381 cgccgaacaa tcagcgaatc tccacgcgtg acagcgcata ccaccggtcc gctcgtttcc
     1441 cctcgattgc caactcgaca tgggcggaga gggcatcttc agagagaatc gccacatcca
     1501 acgcatagga accggcggac agggtctccg gcaccaccac cgtatcctcg acccggggcg
     1561 aaggtcccgg cagccattgc ttgagatcgg caggactggt ccactgcgcc acgacctgat
     1621 ccgagctcga tcgtagccga taggccagcg gccaggcgtg gtagatcggc gccaccccct
     1681 tattctccca acgcgactga aggacgagcg gcccgccggg gtgactctcc gctgaatgcg
     1741 tcagttcgcg caagacgaag cgatacccga tccttttcaa aaattcattg aaccgcggtc
     1801 gccaggcggc aggcaccggc ttcgacttcg cattcagcac cgagagatgc cactccaagc
     1861 cctgattcag aatacggtcg agatcgaacc cgcgatcgta ccactcgtga atgtacccgc
     1921 agacctccat ctgaaccggc ccacgcttcc aggcattcgc aataaccgcc tcttcgagta
     1981 ccggagggta ggcgtgttcc atatgattcc agtcaggact gaaatagccg taatcgccga
     2041 aacaatcccc gcgccaaccg gcgccgtgcg aggccgcata cttcagttgt ccgccgtgga
     2101 gcatcaccaa cggagtgcct gcgaaatatt tcaggtacca gtccgtgatg gcgatctgat
     2161 tcgcctcggt cggaaagaat gccttacact gggcttccac accttcgcaa cacaccgtat
     2221 tccactcgcc ccaacatccg atggacccga tgtcgacatg atcgatatcg acggagcggc
     2281 cgtagcgttc accgaatgcg cggatcagcc gctcatggta gtcgagaaaa acgggatggt
     2341 tgtagtcggg gaaaatgccg tcggactcct tcacactccc gacccccttg tcgagcaacc
     2401 attggggcga gccggttttg tattcggaca cgatgcggat ggcgagtgtc tctcccttgg
     2461 ccttggcctg ctcgatcaca cgatccacga gggcgaagtt gtactgacct tccttcggtt
     2521 caagctctgc ccagggccaa cgaaaatagg cgacggtcgt atggggatac tcttccggtg
     2581 ccggcggatg tccgaacccg aaatgaaaat cggcaaagcc cattccgggg ttgtccagca
     2641 cctcgtcgat ttcacgaggc tgaacggtaa cagtccgccc ggtccccttc acgtcccccg
     2701 cccccgacat gccgctcagt gaatcggcac agccggccgt acccagcaag agcatcacca
     2761 acgtcgcggc gcgccatcct cgtctccgca tgtctcctcc cttctaatcc gggctattca
     2821 tgaatgcctg ctctagcgtc cgatcctctc gcccggcgag gctgttctcg ttcggcctcc
     2881 tcgacggact gcacgtacgc ctgcgtcggc ctcacgtgtg agaagcctcg gcgggcttcc
     2941 gtctcgaacg cctcgcgacg gcattcacac accgttgagc agcttgaaga accggagcgt
     3001 gccgcccgac aaccatccgc cggatgaaac cgagagtagg gtcgcgccgt aaattatgat
     3061 agggtacgcg accggcggaa agaaccgcct cgatatgctg aattggtttt cacaatcgcc
     3121 gcaatccgac tccgtcgtca tcaccggcgc ctccaccggc atcggggccg cgtgtgcgtt
     3181 ggcgctcgac aagctggggt accgtgtctt cgcaggcgtt cgtcatcccg ctgacgggga
     3241 acggctccag caacaagccg gaccgcgact catgccgatt cgcctcgatg tcaccgatgc
     3301 ggcctcgatc tcggcagcca gtcataccgt ggcagccatg gttggagaac gcggcctcgc
     3361 cggcctggtg aacaatgccg gaatcggcgt cgcggggccg atcgaactcc tgcccctcgc
     3421 cgactggcgg cggcaattcg aagtcaacgt gttcggcctc atcgcggtca cgcaaacgtt
     3481 cctcccattg atccgcaccg ggcgaggacg catcatcaat atgggttcca tcgccggacg
     3541 ggcctccatg ccgttcatgg ccccctacgc cgcgtcgaaa catgcgctgg aagcgatcac
     3601 cgacgccttg cgcctggaac tccaaccctg gggcatccgc gtcgcactca tcgcgcccgg
     3661 cgccatcgcc acgcccattt ggaaaaagac cagaaaagaa gtcgatgcct gggacgcagc
     3721 ctgggggcag gacctcaagg gcatgtaccg ggaaggcttc actcgcatca aggacgcggc
     3781 cactgcggcc ggtgaacagg cacaaccggc gagcgtcgtg gccgaaaccg tggcgcatgc
     3841 cttgcgttcg aggtggccga aaacacgcta tctcgtcggc tcggacgccg ccatccgtgc
     3901 ctacctggcg ctgctcctgc cggatcgcct gaatgactgg ctgctcaccc ggatcgtgaa
     3961 gcttcccccg cgccgctga
//
LOCUS       DAORRQ010000156         3976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_656_length_3976_cov_43.046417, whole genome shotgun sequence.
ACCESSION   DAORRQ010000156 DAORRQ010000000
VERSION     DAORRQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_3976_cov_43.046417"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..323)
                     /locus_tag="PK224_20930"
     CDS             complement(15..323)
                     /locus_tag="PK224_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18270.1"
                     /translation="MLNLTWTAIERLRKLIEEHPEDPVVRITLRDLDDQRLSLAITLE
                     PAIQEEDEVQHIEGLTIALERSSVHRTNGMTVDYQADKGFTFVHPPAEELGLIMPSSN
                     "
     gene            complement(326..601)
                     /locus_tag="PK224_20935"
     CDS             complement(326..601)
                     /locus_tag="PK224_20935"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB18271.1"
                     /translation="MPWFRLAFRPEVITDLRVADPAMAQRLFDKTKWLASNVDNLRHE
                     PVAADLPGIHKYAVGDWRIFYSIDRTEQLVDIHHIHHAAPTQPTRPA"
     gene            complement(603..821)
                     /locus_tag="PK224_20940"
     CDS             complement(603..821)
                     /locus_tag="PK224_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18272.1"
                     /translation="MTEPTTPLSNLTLAELKNLVEGIVDDRLRVLLGDPDLGEPLGES
                     VRDRLKQSLTSTERITGDEVADKLGLRW"
     gene            1010..1657
                     /locus_tag="PK224_20945"
     CDS             1010..1657
                     /locus_tag="PK224_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18273.1"
                     /translation="MSLHPDFPSSPYEPLIPDQRWFPADEALRSTAYEKLLPPLVAKV
                     REEVFAWRAKGYAGASATSAALLRHWFESDHLVENADGSLSPFRYYFAQREAVETVIW
                     LFEVRGARDKYDLLRFDASGAVSSDGDLSTYTPDFIVRTIDGTVWVVETKGREELDLP
                     QKMARLKLWCADATAAEENRRRYDFVFVDQLGFEKHTPGTFPSLPSSFTEYKHKS"
     gene            1654..1896
                     /locus_tag="PK224_20950"
     CDS             1654..1896
                     /locus_tag="PK224_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18274.1"
                     /translation="MMTLRQLAAEPPTLPTPTTWYLTDLVEARGKQELFTRQSPQKKP
                     RPCTGLFRPLLHRLLTASIRVHDPDLSFIQQDARAS"
     gene            1893..2957
                     /locus_tag="PK224_20955"
     CDS             1893..2957
                     /locus_tag="PK224_20955"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRB18275.1"
                     /translation="MTSLTQAYLAKLVVPQRIITMIRKLGEHKGKQELYKKQAPEMLE
                     NLRQVALIQSAESSNRIEGIVADDKRLRAIVEEKVKPANRSESEIAGYRDVLNTIHQS
                     HADIPFTERVVLQLHRDLMKYAGGHGGVWKATQNEITETLPGGNTRVRFTPVAPHLTE
                     EFMRTLHGQFANVERVRELDSLLLIPFYVLDFLCIHPFLDGNGRMARLLALLLLYQQG
                     YEVGRYVSLERIIEQSKQSYYDTLYQSSQGWHEGQHDVLPWTEYFLGTLLAAYDEFES
                     RFGTISKGHGSKTDMIRNAVDGFIADFSITEVEKACPKVSRDMIRHVLGQLREAGVII
                     PVGKGRGAKWRNAQPKWGNK"
     gene            complement(3329..3541)
                     /locus_tag="PK224_20960"
     CDS             complement(3329..3541)
                     /locus_tag="PK224_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18276.1"
                     /translation="MEGLTADLDVHAEVRAHVEGRVDVDELQASGVFDLAAERAALEG
                     RQNQLVVAPNEFVGPTLNLPPAHIKV"
     gene            complement(3717..>3976)
                     /locus_tag="PK224_20965"
     CDS             complement(3717..>3976)
                     /locus_tag="PK224_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRB18277.1"
                     /translation="CRCVSGLLETLKPEYREALRIVDLEEGKLKDLAQQSGITAENAA
                     VRVHRARAALRRRIEQACGTCSVHGCLDCSCEKADGEPCSG"
BASE COUNT          831 a         1162 c         1174 g          809 t
ORIGIN      
        1 cgtttcacca ggcttcagtt gctgctgggc atgatgagcc ctaattcctc tgccggcgga
       61 tgcacgaagg tgaatccctt gtctgcctga taatccaccg tcattccgtt cgtacggtgc
      121 acgctcgaac gctccagcgc aatcgttaat ccttcgatgt gctgcacttc gtcttcttcc
      181 tggatagccg gttccagcgt gatcgccaag gagaggcgct ggtcatcgag gtcgcgcagc
      241 gtgatgcgca cgacgggatc ttccggatgc tcctcgatca gcttgcgcaa ccgttcgatg
      301 gcggtccagg tgagattcag catggtcagg ccggtcttgt cggctgtgtc ggcgctgcgt
      361 ggtggatgtg gtgaatgtcc acgagttgtt cggtccggtc gatcgaataa aaaatccgcc
      421 agtcgccgac ggcgtactta tggatcccgg gcaggtcggc agcgaccggt tcatggcgca
      481 gattgtcgac gttagatgcg agccacttcg tcttgtcgaa caggcgctga gccatggcgg
      541 gatccgccac gcgcaagtcg gtgatcacct cgggccggaa ggctaagcga aaccagggca
      601 tgttaccacc gcaggccgag cttgtcggcc acttcgtcac cggtgatccg ttccgtcgag
      661 gtcaacgact gcttgaggcg atcacgaacc gattcgccca gcggctcgcc gagatccggg
      721 tcgcccagca gcacgcgcag ccggtcgtca acgatgcctt cgaccaggtt cttgagttcc
      781 gccagcgtga gattggagag gggcgttgtg ggttccgtca ttgtgtcacc gaaatcatgc
      841 tgggagcata cggggctggt ctctggaatt gcaagacagg ggtggaatcg tgaggcgtat
      901 ctcggccgac gtgtgtagcc ggaccacgtg atttctcact cagaacaagg tattctgcgc
      961 ccgcgtaagc gggcgatcaa tcaaaggcaa cgtccaaagg caacgtcgca tgtctcttca
     1021 cccagatttc ccaagctcgc cctacgaacc actcatcccc gaccagcgct ggttcccagc
     1081 cgacgaagcc ttgagaagca cggcctatga gaaattgctg ccgccgttgg tcgccaaggt
     1141 gcgggaggaa gtctttgcct ggcgagccaa aggatatgcc ggtgcgtctg ccacttcggc
     1201 agcgttgctc cggcactggt tcgagagcga tcacctggtc gaaaacgccg atggttctct
     1261 ctcccccttc cgttactact ttgctcagcg cgaggcggtg gagacggtca tctggctgtt
     1321 tgaggtccgt ggcgcgcgcg acaagtacga tctgctccga ttcgacgcgt ccggcgcggt
     1381 ctcgtccgac ggcgacctct ccacctatac gccggacttt atcgtccgca ccatcgacgg
     1441 cacagtctgg gtcgtcgaga ccaagggacg agaggagttg gatctgccgc agaaaatggc
     1501 gcggctcaag ctgtggtgtg ccgacgcgac ggctgccgaa gagaaccgac ggcggtacga
     1561 cttcgtcttc gtggatcaac taggatttga gaaacatacg ccgggcacgt ttccctcgct
     1621 cccatcaagc tttacggagt acaaacacaa atcatgatga cgctccgcca actcgctgcc
     1681 gaaccgccca cgttgcccac gccgacgacg tggtatctga ccgatctcgt cgaggccaga
     1741 ggcaagcagg aattgttcac ccgccagtcg ccgcagaaga aaccacggcc gtgtactggc
     1801 ttgttccgcc ctctccttca ccgactcctg accgcgtcca ttcgcgtcca cgatccggat
     1861 ctgtcattca tccaacagga tgcccgagca tcatgacttc attgacacag gcctatctgg
     1921 ccaagctggt cgttccgcag cggatcatta cgatgattcg caagctgggc gaacacaagg
     1981 gcaaacagga actctataag aagcaggcgc cggagatgct ggagaatctc cggcaggtgg
     2041 ccttgattca gagtgccgag tcctccaacc gtattgaagg cattgtcgcg gacgacaagc
     2101 ggctgcgggc cattgtcgaa gagaaagtga aacctgccaa ccgttccgaa tcagagatcg
     2161 ccggctatcg ggatgtgctc aatacgatcc accagagtca tgcggatatt ccctttaccg
     2221 agcgtgtggt cctccagctc caccgcgact tgatgaagta tgccggtggt catggtggcg
     2281 tctggaaggc cacgcagaac gaaattaccg agacgttgcc ggggggaaac acgcgtgtcc
     2341 ggtttacacc ggtcgccccg catctgaccg aggagttcat gcggacgttg catgggcaat
     2401 tcgctaatgt ggaacgggtg cgagaattgg attcgctgct cctcattccc ttctacgtgt
     2461 tggactttct ctgcatccat ccctttctgg atggcaatgg gcggatggct cgcctgcttg
     2521 cgttgctgct gctctaccaa cagggctatg aggttggacg gtatgtgagt cttgagcgga
     2581 tcatcgagca gtcgaagcag tcctactacg acactctgta tcagtcatca cagggatggc
     2641 acgagggcca gcatgacgtt ctcccctgga cggaatactt tctcgggacc cttctggcgg
     2701 cctacgacga attcgaaagc cggttcggca cgatcagcaa ggggcacggc agcaagaccg
     2761 atatgatccg caatgcggta gacggtttta tcgccgactt cagtattacc gaggttgaaa
     2821 aggcctgccc taaagtcagc cgggacatga ttcgccatgt gctcggccaa ctccgggagg
     2881 ccggcgtcat cattccggtc gggaaaggcc gcggagcgaa atggcgtaat gcgcagccga
     2941 aatggggtaa taagtaaggg gttattaccc cgtttctaac ggttcttacc gcatgtatgc
     3001 ccggggcatg gcggtggaaa caggtagtac ccgatcaaat gggtactgaa ttgggtacta
     3061 cccttaaaag agggtaaaag agagggtaat agctaggaca tggcgcgacc gcacgatcct
     3121 ctccgacgcc gagaagcgcg acctcatcaa gctcatcgag caggggaagc cgctgccgga
     3181 gaagtaccgc tgcctcctct ttgccgacaa acgcgaggtg gaactggtct ggaaaggcaa
     3241 gtcccgcgaa gtctgcaccg ccatcctgcc cttacagacc ctcgaacaca ttgacgagcc
     3301 ccggaaggaa cagcgcggtg atggggaatt agaccttgat atgggcgggc ggcaggttaa
     3361 gggttggacc aacaaactca tttggggcga caacaagctg attctgtctt ccctcaagag
     3421 cggcgcgctc cgccgccaga tcgaagacgc cggaggcttg aagctcatct acatcgaccc
     3481 gcccttcgac gtgggcgcgg acttcagcat ggacatcgag atcggcggtg agaccttcca
     3541 caaagagccg aacctcctcg aacagatcgc ctaccgcgat acctggccgc tatgtcattg
     3601 ccgtcaaagt catcgacatc ttcggcaacg acaccatgac gctcatgcct atcacagtgg
     3661 ggtgattggc ccagcaaagc cgtgtgtaac tgcattgatc cgggcaggtg gagcgttcac
     3721 ccactgcacg gctccccgtc ggctttctca caggaacaat ccaggcaacc atggacggaa
     3781 caggtgccgc aggcctgttc gatccggcga cgcagcgcgg ccctggctcg atgcacgcga
     3841 accgccgcat tttcggcggt gatgcctgac tgctgagcaa gatctttcag tttgccttcc
     3901 tccagatcca cgatccgcag ggcttcgcga tattccggct tgagcgtttc cagcaggccc
     3961 gagacgcatc ggcaga
//
LOCUS       DAORRQ010000157         3862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_666_length_3862_cov_7.575781, whole genome shotgun sequence.
ACCESSION   DAORRQ010000157 DAORRQ010000000
VERSION     DAORRQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_666_length_3862_cov_7.575781"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1160)
                     /locus_tag="PK224_20970"
                     /pseudo
     CDS             complement(1..1160)
                     /locus_tag="PK224_20970"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            complement(1357..2070)
                     /locus_tag="PK224_20975"
     CDS             complement(1357..2070)
                     /locus_tag="PK224_20975"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HRB18278.1"
                     /translation="MGELVIHRHPSSFLSVGLSLQLSVPQDDTRGQYGSSVLGWKHRS
                     WIVCEWPFHLGQPIPCVKGTRCLLRYIHEGNMIGYRTEVLDTQMQPFPFLLLAFPSEL
                     EEVPLRKHGRVSAQEPIVLSVVREFASNLPHEPPIRIGGLLKDLSASGCAVLIQRPLQ
                     DFYPGMALRVEFEIIGTGHVNNLTGVVRNLSLQADGTHLGLEFRFDGKETIEYRGWGG
                     SVRNALESFLHRKHSFESA"
     gene            2282..3535
                     /locus_tag="PK224_20980"
     CDS             2282..3535
                     /locus_tag="PK224_20980"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HRB18279.1"
                     /translation="MASTKHISIDQLTVGMFIAGLDQPWYRTPFLLHKWLVSNPDDIV
                     QLKRHGILVVTIDTDRGLDVGAAAPPAPATAPPPDEPPPVSVEAAVPQESEQSPHAAV
                     AAAAYRESMAAVERVFSDLEAGNPPKIAALKPIVSRLLEQIVAQPEAMLIQFCLDKVR
                     RFDATLANHGMDVCVLTLILAVENGCAEADLESLGLGALLHDIGYVRLPRNLYRKTTP
                     LTDQEQVLMRQHPQLAATVLAQVGAIPDAVSRIILQHHEYQDGSGFPQHLKQDGLSTL
                     AQLVGLADSYDDLVSARHGRPPLLPHDAIRQLFVLGAKGRYEKTLVEVAIKALGVYPL
                     GSLIKLNTNECALVVGLNHEDRLRPRIRIIKGAEGQSQQEPLEIDLQNQSPDQPTRTI
                     LRALDPKLEQVDLPHYLEPAVGARS"
     gene            3532..>3862
                     /locus_tag="PK224_20985"
     CDS             3532..>3862
                     /locus_tag="PK224_20985"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRB18280.1"
                     /translation="MTNHPQHPRTPQPQHAAGLAVPPEVLTLLWDAVPLGICLLGAEH
                     TVIFANRTAARLFGRSPGACAGQALSDLLGQRLDLSDSLRATGIVKLPDGPFSHAGQA
                     ASHDESDT"
BASE COUNT          797 a         1167 c         1135 g          763 t
ORIGIN      
        1 gccacatcct gttcgttcat ggaggcgtga ttgggcagga agaagccgca atgatgtacg
       61 cggtctgcca cagggaagct ggccttcccg tagcggttca tccagaaagg atgaagcccc
      121 agattgccgg cggagaaaat tctcgtttcc accccttctg tgaccaatgc ccggacaatc
      181 cgacggcgtt gttccgtcga atctgcgagc aacccgaagg agatgctggc aactttgctc
      241 cctgtcggcg gccgctgagt gtaaaaccgg ctacccaagt gctacaggta ccgttcgtga
      301 ttggcctgcc gtcggccggt catccagtcc agcttgtgca gttgctcgat gccgatgaag
      361 gcgttgagat cggtcgagcg gagattgaag cccggctcgt aaaacacaaa aggcgagtgg
      421 aaatcgtcaa cctggtattg cgtcagtaac gcctgatggc tgctctggtc gaggtccttg
      481 ctccacccat ggctgcgcag catcaagagc atgtccgcca actgcttgtc gctggtcgac
      541 accatgccgc cttcaatcgt ggacatctgg tggccgaaat agaaggagaa gctcgccatg
      601 tcgccgaatg tgccgatctt cttgccgcca tattccgcgc cgatggcggc acaggcatct
      661 tcgagcagat aaaacccgta ccggtccttc agggtctgca gctcctgcat gcgatgcggg
      721 actcccagta cctggacgag taacaccgtc tgcgcgtcat ggcgttgtaa gaggtcttcc
      781 agatggttga ggtcgaggcc gaaggtatcc ggatcggcct cgcacatgat gggtgtaaag
      841 ccgaattgaa ttgccggggc gatggacgtc acccatccca cactcgggac gatcacggtt
      901 gtgttgcgca gcttgcctga gcgcagcaat gcgtaataca tcaaaagatt ggccgacgag
      961 ccggagttgc agtgcaccga atagggacgc cctaaccatt ccgaccattg ccgctcgaag
     1021 tcgagggtca ccgccccttt ggtgaggcga ggataggtcc gcagccactc gatcaaatgg
     1081 tcgatatcct gccggtcgat cgtatcctgc gcgaggcacc agcgaggatt gagcttggca
     1141 ctctgtgcgt gaagcgtcat gattgtcgtc cctcttgctc ctgaacgtgg cctgcgtccc
     1201 tcacaggacg aggtgtggca acgggatacc aatcaagatg tatgccactg acgtgtgcgg
     1261 cgggaggaat tgaaacgacg ggatttctgg gcgtagggaa cgagtcgccg caaacgaccg
     1321 ggcaaaaact tcccagtcaa cggcaaccgc tgcagtctag gcggactcga aggagtgttt
     1381 ccgatgcagg aaggattcaa gcgcgttccg caccgatcct ccccatcccc gatactcgat
     1441 cgtttctttg ccgtcgaacc ggaattccag gccgaggtgc gttccgtcgg cctgcagtga
     1501 caggtttcgg acgacccctg tcagattgtt gacgtgtccg gtgccgatga tttcgaactc
     1561 aacacgcaga gccatccccg ggtaaaagtc ctgaagcggg cgttgaatga gtacggcgca
     1621 gccggaggca ctcaaatcct tcaggagccc gccgatgcgg atcgggggtt catgtggcag
     1681 gttggatgcg aactcccgca cgacagacaa gacaatcggc tcctgggccg atacccgccc
     1741 gtgcttccgg agcggcacct cttccagctc ggagggaaat gcgagcagta gaaacgggaa
     1801 gggctgcatc tgcgtgtcca gcacctccgt gcgatatccg atcatgtttc cttcatggat
     1861 gtaccgcaac aggcagcgcg ttcctttcac acagggaatc ggttgcccca gatgaaacgg
     1921 ccattcgcag acgatccagc tccggtgttt ccagcccagg accgaggagc cgtattgccc
     1981 ccgtgtgtcg tcctgcggca cgctgagctg aagcgagaga ccgaccgata gaaacgagga
     2041 cggatgacga tgaatgacca gttcgcccat agcctcttcc cccaccctgc aggcgcgtag
     2101 acgcaccctc gtcagctcta tcggtcgcct tccgcaagac ctgaaggacg cggtagatct
     2161 gtggtgtcgc acaggtaaag accggcctct gatcaaccga tatgaacgtt acatcataga
     2221 tgagacaccg gaaacactgc gctcagttca atccgtcctg aggagtaggt acagcgctga
     2281 tatggcttcg acaaagcaca tctccatcga tcagctcacc gtcggcatgt ttatcgccgg
     2341 tttggatcag ccttggtacc gcactccgtt tctcctccat aagtggctgg tttcaaatcc
     2401 cgacgacatc gtgcaactca aacgccatgg catcctcgtc gtcaccatcg atactgaccg
     2461 cgggctggac gtcggggctg ccgccccgcc agcgccggcc actgcgcctc ctccggatga
     2521 accgccaccg gtcagcgttg aagccgccgt gccacaggag agcgagcaga gtccgcacgc
     2581 tgcagtggct gccgcggcct atcgggaaag catggccgcc gtggaacgcg tcttcagtga
     2641 cctcgaagcg ggtaacccgc cgaagatcgc ggccctcaag ccgatcgtga gcaggctact
     2701 ggagcagatc gtggcacagc cggaagcgat gctgatccag ttctgtctgg ataaagtccg
     2761 acgatttgac gccaccctcg ccaatcatgg catggatgtg tgcgtgctca ccctcattct
     2821 tgccgtggaa aacggctgtg cggaagccga cctggaatct cttggactgg gggctctgct
     2881 tcatgacatc ggctatgtgc gactcccgcg caacctgtat cgaaagacca ccccgctgac
     2941 cgatcaggag caggtcctca tgagacagca cccgcaattg gcggctaccg tactcgctca
     3001 agtgggcgcg atccccgatg cggtctctcg cattatcctg cagcatcatg aatatcagga
     3061 cggaagcggg tttcctcaac acctgaaaca ggacggtctc tccaccctgg cgcaactggt
     3121 gggtttggcg gacagctacg acgatttagt cagtgcgcgc catggccgtc cgccgctgtt
     3181 gccgcacgat gcgatccggc agctcttcgt cttgggagcg aaaggccgat atgagaagac
     3241 gctggtggag gtcgccatca aggccttggg cgtctaccct ctcgggagtc tcatcaagct
     3301 gaataccaac gaatgcgcat tggtcgtcgg tttgaatcat gaagatcggt tgcggccacg
     3361 tatccggatc atcaagggcg ctgaagggca gagtcagcag gaacccctcg aaatcgacct
     3421 gcaaaatcaa tcgcccgacc aaccgacccg caccattctt cgggcattgg atccaaagct
     3481 ggaacaggtc gacctcccgc actaccttga acctgccgtg ggtgctcgct cgtgacgaat
     3541 catccgcagc atccacgcac cccgcagccc cagcacgcag ccggcctggc cgtcccaccg
     3601 gaggtcttaa ccctgctgtg ggatgcggtg ccgctgggca tctgtcttct gggggccgag
     3661 cacaccgtta ttttcgcgaa tcggaccgct gctcgtctgt tcgggcgatc tccgggggcc
     3721 tgtgccggac aagcgctgtc ggatctcctc ggtcagcggc tcgatctgag tgactcgctt
     3781 cgcgccacgg gaatcgtgaa actgcctgac gggccgtttt ctcacgccgg ccaggcggcc
     3841 agtcacgatg agagcgatac gg
//
LOCUS       DAORRQ010000158         3767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_680_length_3767_cov_25.935075, whole genome shotgun sequence.
ACCESSION   DAORRQ010000158 DAORRQ010000000
VERSION     DAORRQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_680_length_3767_cov_25.935075"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK224_20990"
     CDS             <1..333
                     /locus_tag="PK224_20990"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRB18281.1"
                     /translation="RDLLRTFAQSAAYDRLRRATVIGREVPFLVPWNGGRQIMEGVID
                     LLYRIDGALWIADYKTDMIPADQVAARAELYREQARLYRAAVEQSSGEPVAGFEFIFL
                     RQGVAVTV"
     gene            351..755
                     /locus_tag="PK224_20995"
     CDS             351..755
                     /locus_tag="PK224_20995"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB18282.1"
                     /translation="MRAFRWAVLVMMAVVGCASQHKAEVRPLHAAAGTSAVVSQQLEQ
                     GNQLFAQQDWAGAQQVYLATIQADQTLAEAHYNLALTLERLGEKAEARKHYVAAANLA
                     PGNKVIWDAPPLRKYDRELGLDRKSWMDANPK"
     gene            complement(781..1614)
                     /gene="lgt"
                     /locus_tag="PK224_21000"
     CDS             complement(781..1614)
                     /gene="lgt"
                     /locus_tag="PK224_21000"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRB18283.1"
                     /translation="MLSWLHAIPYPDIDPVFLRVGPLQFRWYGLMYLIGLTLAYFIIA
                     ARARAQKLPMNNDQVYDMIVYAAVGVFAGGRLGYVLFYNLSYYLENPLKIFAVWEGGM
                     SFHGGLIGTIVALILFARRQGMTVLTIADLAAGVTPVGLGLGRIGNFINGELYGRPTD
                     VDWCMVFPAGGMACRHPSQLYEAFLEGLLLFTVLWLITRRLPPSGTVFGAFLMGYGIC
                     RIVVEFFREPDAQIGFLVGTISMGQMLSIPMIVAGAVILAIAAQRKRPLQPDSGTAAP
                     L"
     gene            1941..2192
                     /locus_tag="PK224_21005"
     CDS             1941..2192
                     /locus_tag="PK224_21005"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRB18284.1"
                     /translation="MSTKGREAAKIAAIIGGGAVVGAALGLLLAPKTGAETRRDVARY
                     AKRAQVQATRFSRSVKSGVSTMVERSKALVKRDDHKEAA"
     gene            2348..2434
                     /locus_tag="PK224_21010"
     tRNA            2348..2434
                     /locus_tag="PK224_21010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2382..2384,aa:Leu,seq:taa)
     gene            complement(2826..3254)
                     /locus_tag="PK224_21015"
     CDS             complement(2826..3254)
                     /locus_tag="PK224_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18285.1"
                     /translation="MMALVVASGGTEQAMPFVQSAHYGFSGVITKIESGMLFVETDTS
                     LQARVISPTKAERVGLYDAKIGDTVNMLVDSDNVLMDISRTDQFFPEHHFVAGRLHYA
                     DPLRGAIQLSTPEGPASFDVDPLAGANSPRCRTAPPSPLS"
BASE COUNT          800 a         1066 c         1144 g          757 t
ORIGIN      
        1 cgggacctgt tgcgaacctt cgcccaatct gcggcctacg atcgactgcg gcgggcgacg
       61 gtgatcgggc gcgaggtccc gtttctcgtg ccatggaacg gcggtcgtca gatcatggaa
      121 ggggtcatcg atctgctgta ccggatcgac ggcgcgctct ggattgccga ttataagacg
      181 gatatgatcc cggccgacca ggtggcggca agggctgagc tctatcgtga acaggcgcgc
      241 ctgtatcggg cggcggtcga acagtcgtcg ggcgaaccgg tggcgggttt tgaatttatt
      301 ttcctgcgac agggagtggc ggtcacggtc tgagtgcaaa ggagggtggc atgagggcgt
      361 ttcgatgggc ggtgttggtc atgatggcgg tcgtcggctg tgcgagtcaa cacaaggcgg
      421 aggtgcggcc gctgcatgcg gcggccggga ccagcgcggt ggtgagtcaa cagttggagc
      481 agggcaatca gttgtttgca caacaggact gggccggggc tcagcaggtg tatctggcga
      541 cgatccaggc cgaccagacg ctggcggaag cccactataa tctcgcgtta acgctggagc
      601 gattgggcga gaaagcggaa gccaggaaac actatgtcgc cgcggcgaac ctggctccag
      661 ggaacaaggt gatttgggat gccccgccgc ttcggaaata tgaccgcgag ctcggattgg
      721 acagaaaatc ctggatggat gccaatccca agtagcagtg ccgtaacggc cgcccacgct
      781 tcagagcggg gcggctgtac ctgagtccgg ctgaagcgga cgtttccgct gggccgcgat
      841 cgccaggatc accgctcccg ccacgatcat gggaatgctg agcatctgtc ccatcgaaat
      901 cgtaccgacg aggaatccaa tctgcgcatc cggctcgcga aagaactcca ccacaatccg
      961 gcagatccca tagcccatca ggaaggcgcc gaagacggtg cccgatggcg gcaggcgtcg
     1021 ggtgatcagc cagagtacgg taaagagcag gagcccttcc aggaacgctt catagagctg
     1081 tgacgggtgg cggcaggcca taccgccggc cgggaacacc atgcaccagt ccacatctgt
     1141 ggggcggccg tagagttcgc cgttgataaa gttaccaatt cgccccagtc cgagacccac
     1201 cggggtcaca ccggcggcga ggtcggcaat ggtcagaacc gtcatacctt gccgcctggc
     1261 aaacagtatc aacgccacaa tggtgccgat cagcccgcca tgaaacgaca tgccgccttc
     1321 ccagacggca aaaatcttga gcggattctc caggtagtaa gacaggttat agaacagcac
     1381 gtaacccaac cggcccccgg cgaagacgcc gactgctgca tagacgatca tgtcgtagac
     1441 ctgatcgttg ttcatcggca gcttctgcgc cctggcccgg gccgcgatga tgaaataggc
     1501 cagcgtgagg ccgatgagat acatcaggcc gtaccaccgg aactgaagcg gccctacgcg
     1561 caggaagacc gggtcgatgt cgggataggg gatggcgtgc aaccaggaca gcatagtagg
     1621 actccacata gtttcgcgat cttatgtgag cctgttctgc aatgcaaggc agggcgcgaa
     1681 gcggcgccgg gcaaggcctg cagccggttc agctgttttc cctacgcaca cctgtcgctt
     1741 ttctgaaacg gttgtctcct tccgttgttc acaaaacagc accctttatg agaagtcgtt
     1801 aacccggtgt cgccgtaacg cagaaatccc aacgaatccg catcgtgcgg ctgtggcacg
     1861 gttgatgctg tactcatcgg tagctctcgc cggaagtcag tggacagtaa accttgtggt
     1921 actaaaggga ggatgtcacg atgtctacca aggggcgtga agcagcgaag attgcagcca
     1981 tcatcggagg gggcgcagtt gtgggggcgg cgctgggatt actcctggct ccgaagaccg
     2041 gggcggaaac gaggcgggat gttgcgcgat atgccaagcg ggcgcaagtc caggccaccc
     2101 gtttcagtcg ttccgtgaag agcggcgttt ccaccatggt ggaacgtagc aaggcattgg
     2161 tgaagaggga cgatcataaa gaagccgcgt aagcctggtg gtttgccggc accggttctg
     2221 acaaaagggg catactgcag cggcctgtct cctgagggac aggccgtttg catgtgagca
     2281 gatgttcgtc cgggcagctt gaccccccgg gagcccgatg ttagatttcg ccccggcagt
     2341 ttttactgcg ggagtggcgg aatcggcaga cgcccaggac ttaaaatcct gtactgtaaa
     2401 aagtgtgcgg gttcaagtcc cgcctcccgc accaaaaccc caacgctttt cttccacgac
     2461 cggccccatt tgcgttcctc caagttctcc ccaaccttgc gagtgtgaaa actcctcccg
     2521 tcggatccac tttcggacca atcgcagaac gccgatacat gctctctctc gtgtgcctgg
     2581 ttcatttctg ccgggaaatg gtgggcgccc gctctttccg gtttccgaga acagtctcgg
     2641 agaaattgta aggtcccctc aaccgtgtcc catccgttag accagaagcg accgacaggt
     2701 cttcgtgagg tcagtgtctc gcgtcgcctg tcgcgctagc aaattgtgat cactgattga
     2761 ggctaaggtg tgtgtctgcc gggtcgcgca ctcgatcgtg ccatccaaca tgacgttatc
     2821 cgcgtctagc tcaatggcga cgggggtgcc gtcctgcaac gtggagagtt tgctcccgcc
     2881 agtggatcca cgtcaaaact tgccggccct tcgggggttg aaagctgaat ggcgccccga
     2941 aggggatccg catagtgaag ccgaccggca acaaagtgat gctcagggaa gaactgatct
     3001 gtccgtgaga tatccatcag aacgttgtct gaatcgacga gcatgttcac ggtgtctccg
     3061 atcttggcgt cgtacaaacc gacacgctcc gccttggtgg gactgatcac acgcgcctgc
     3121 aagcttgtgt cggtctctac gaagagcatg cctgattcaa tcttggtgat caccccgctg
     3181 aagccgtaat gcgcgctctg cacaaagggc attgcctgct cagtgccgcc tgaggcaaca
     3241 accagcgcca tcatcgctat cgtcagaaag agggaacgtg taggtttcag cggtctaccc
     3301 tcctcgcata gccattgaag aacaatcact gaagcactgg aactgtagac agcttgatgc
     3361 cccctgaccc tcgcccgaag gatcgatttt actcgtgaac ggctaatttc tatcgacgcc
     3421 acagttttgt ttgccgcgcg gcagacaagt ccggaagagc catgtgcgcc ctaacgatca
     3481 gcctgcttga gcccacagct gtagccgcct acgcttggta ccatcatttt gattacttaa
     3541 gaattcctcc taccgcacgc ctacctgtca ttttcagcgg gagagggttc cagaaattcg
     3601 gacaatgtgc gaagtgatag cctagcttca ccgagtattt gcggaacgac tctggttctt
     3661 gcggtcaagc gaccggtacg gtctggtctg gctagagaac gtcaggtagc gtgtgatctt
     3721 cccccgattt cgtggacacg tggttacgcg actgccgccc tcgcttc
//
LOCUS       DAORRQ010000159         3650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_697_length_3650_cov_112.844506, whole genome shotgun sequence.
ACCESSION   DAORRQ010000159 DAORRQ010000000
VERSION     DAORRQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_3650_cov_112.844506"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PK224_21020"
     CDS             <1..788
                     /locus_tag="PK224_21020"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRB18286.1"
                     /translation="SWSVYALAAAAVTGAFVEAALLGWQLKRLGIALLPQWHGLSPAT
                     RQVLGQYWPMVAAAFLMGSTHLISQGMAAMLDPGSVSALGYGNKVTAVLLGIGATAVG
                     TAFLPHFSIMAAAGDWIGMRQTLFTYMGLLLAATIPLTAFLIYLSEPVVGILFERGAF
                     NAADTHIVGQVQAAYLLQVPFYLIGMLFVRCVTALRSTQVMVWGNIINLMACVVLTYW
                     FMRLFGVVGIALATSAMYGVSCVFLMVVSWTLLQAHMRRAEVA"
     gene            813..1952
                     /locus_tag="PK224_21025"
     CDS             813..1952
                     /locus_tag="PK224_21025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRB18287.1"
                     /translation="MRITLVISTFGAGGAERVMAVMANYWAERGHDITLVTLARSDED
                     FYPLHAAVKRVGLGVMKVSSGLLEALWNNLVRLKQLRDAIRLSRPDVVISFIERTNIL
                     VLLSTIGVSVPIVACERIDPRHHSIGPVWSGLRRCLYRRAASLVVQTEGLRSWAEGFV
                     PARSVHVIPNPIATVSEEASAERATGGRSKCVSALGRLVPQKGFDTLLQAFAHCVRTH
                     ADWSLTIIGEGPERSQLEALSARLGIGDCVSMIGRRSNPFPILRQTDLFVLSSRYEGF
                     PMALVEAMACGLPVISTDCPSGPRDIIRDGVDGILVPPDDVPALTQAMTRLMENSDER
                     HRLRMRAGEVVDRFSTERIMALWGKLVTDVVGASPSWSVTPGRAV"
     gene            1949..3100
                     /locus_tag="PK224_21030"
     CDS             1949..3100
                     /locus_tag="PK224_21030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB18288.1"
                     /translation="MKVLFLTRALNVGGAERQLVALATGLRKRGHEVAVAVFYPDGVL
                     EGELKEAGIPILSLAKRGRWDVVGFVLRLIHLVRCYRPDVLHSYISNLVTVVVQPFLT
                     SSKIVWGVRSSYMDFSRYDWLFRVSYGLACRLSHSADLIIANSHAGRRGHVADGYPAG
                     KMVVIPNGIDVERFHPNIEARARIREEWGLSEEDALIGIVGRLDPMKDVETFLAAAAA
                     IASTCQAVHFVSVGDGRPEYRAALQERARTLGLTGRLRWAGTRTDVADIYSALDVLVN
                     CSYGEGFSNVIGEAMACGIPCVATNVGDSGVIIGSLGEVVEPKDAVALAQGIERLLQR
                     RLTSAEIRQRMVDHFSLDTLVLNTERELLALCGSSTGNRIPARENPTIG"
     gene            3130..>3650
                     /locus_tag="PK224_21035"
     CDS             3130..>3650
                     /locus_tag="PK224_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18289.1"
                     /translation="MNSTIRIVYAVCAVAVFYGNVHNYLHDHHGIGVPWHWVLAFIVF
                     SLPVLFKQVMQTDLLRQPIVLWCLGYAWLTMVWFLWGPQSDMAWQEVRWRVLAIVEML
                     SFLAVFMDAQADRFARITLVIGVHVGIALNIYELFVPMSFSNVFGRSAGLYLSPTLTK
                     MSSGKYIFISTYI"
BASE COUNT          642 a          939 c         1151 g          918 t
ORIGIN      
        1 gctcctggtc ggtgtatgcc ctggcggctg cagccgtgac gggagcgttc gtcgaagcgg
       61 cgttgctggg ttggcaattg aaacggttgg gaatcgcgtt gctcccacag tggcatggcc
      121 tcagtccggc aaccaggcag gtgctcggac agtattggcc gatggttgcg gctgcatttc
      181 tgatgggtag tacacatctc atcagtcaag ggatggctgc catgctggat ccgggtagcg
      241 tgtcggcgct ggggtatggg aacaaagtaa ctgcggtcct gctcggcatc ggggcaacgg
      301 cggtgggcac agcgtttctg ccgcattttt ccatcatggc tgcggccggt gattggattg
      361 gtatgcggca gaccctattc acttatatgg ggttgttgct tgcggcgaca attccgctga
      421 ctgcctttct gatctatctg tccgaacccg ttgtaggcat cttgtttgaa cggggcgctt
      481 ttaatgctgc agatacccac attgtgggac aggttcaggc tgcctatctg ctgcaggttc
      541 cgttttatct gatcggcatg ctgttcgtcc gatgtgtgac cgcacttcgt tcgacgcagg
      601 taatggtctg gggcaacatc atcaacctga tggcctgtgt tgtgctgact tactggttca
      661 tgcgtctgtt tggggtggta ggtatcgccc tcgcaaccag cgcaatgtac ggcgtttcct
      721 gtgtgttcct gatggtggtt tcctggacat tgttgcaggc tcatatgcga cgtgccgagg
      781 tagcctaaga gtcatcctca ttttcctcat ttatgcgcat cacactggtc atttcgacat
      841 tcggagccgg cggggctgag cgggtaatgg ccgtcatggc caactattgg gccgagcgtg
      901 gtcatgacat cacgcttgtg acgctggctc gctccgacga agacttctat ccgcttcatg
      961 cggcggtcaa gcgggtaggc ctcggcgtga tgaaggtttc ctccggtctt ctggaggcat
     1021 tgtggaacaa tctcgtgagg ctcaaacagc tgcgggatgc cattcgtctc tcacgacctg
     1081 acgtggtcat cagcttcatt gagagaacta atattcttgt gctgctctcg acgatcggcg
     1141 tatcggttcc catcgtcgcc tgcgaacgca tcgacccccg ccaccattcc atcggcccgg
     1201 tgtggtccgg gcttcgacgg tgcctgtaca gaagggcggc ttcgttggtg gtccagaccg
     1261 aagggctccg ctcctgggcc gagggctttg tacctgcccg atctgtacat gtgattccga
     1321 accccattgc gacagtctcc gaggaggcct cggcggaacg tgcgaccggg ggaagaagca
     1381 aatgcgtgtc ggcacttggc agactggttc cacagaaggg gtttgacacg ttgcttcagg
     1441 cgtttgcaca ttgcgtacga acacacgcag actggtctct cacgattatc ggagaaggtc
     1501 cggagcgatc ccaattggag gctctctcgg cccgattggg catcggagat tgcgtgtcca
     1561 tgatcggccg ccgatccaac ccctttccga tccttcgcca gacagatctg tttgtgttgt
     1621 cctcccggta tgaagggttt ccgatggcgc tggttgaggc catggcctgc ggccttcccg
     1681 tcattagcac agattgcccg agcggtcccc gggatattat tcgcgacgga gtggacggga
     1741 ttctggttcc gccagacgat gtgccggctt tgactcaggc catgactcgt ttgatggaaa
     1801 attccgatga gcgtcatcga ttgaggatgc gagccgggga ggtcgtcgat cgtttcagca
     1861 ccgagcgaat catggccttg tggggaaagt tagtcacgga cgtcgtcggc gcttcacctt
     1921 cgtggtctgt cactccagga cgggcagtat gaaggttctg tttctgacgc gtgctttgaa
     1981 tgttggcggt gcggaacgcc agctggtcgc gttggctaca gggcttcgaa aacgaggaca
     2041 tgaggtggcg gtggcggtgt tttatccgga cggtgtgttg gagggagaac tgaaggaggc
     2101 cggcattccc atcctttcat tggcaaagcg cggacgttgg gatgtcgtgg ggttcgtgct
     2161 gaggttgatt cacctagtgc gatgctatag acccgacgtc ctgcatagct acattagcaa
     2221 cctcgttacg gtggtagtgc agccgttcct gacgtcgagc aagatcgtgt ggggagtgcg
     2281 gagttcctac atggatttca gccggtatga ttggctcttc cgtgtcagtt acgggttggc
     2341 gtgccggctg tctcactcgg ctgatttgat cattgccaac tcccatgctg gacgacgagg
     2401 ccacgtcgcc gacggatatc cggctggaaa aatggtggtg attccgaacg ggatcgacgt
     2461 tgaacgattc catcctaata ttgaggctcg ggcgaggatt cgagaggagt ggggactgtc
     2521 ggaggaagat gcgttgatcg gcattgtggg ccggcttgat ccgatgaaag acgtggagac
     2581 gtttctggcg gctgcggccg cgattgcctc gacgtgtcaa gccgtccatt ttgtctccgt
     2641 cggcgacgga cgtcctgagt acagggctgc gttgcaagaa cgagcacgca ccctcggcct
     2701 caccggtcgc ctccgctggg ccggcacgcg gacggatgtg gccgacatct atagtgcgct
     2761 cgatgtgtta gtgaactgtt cctatgggga aggtttctcc aacgtgatcg gagaggcgat
     2821 ggcttgtggt attccctgtg tggcgaccaa cgtcggggat tccggcgtca tcatcgggtc
     2881 gttgggcgag gtggtcgagc ccaaggatgc tgtggcgctc gcgcaaggca tcgaacggct
     2941 gctgcaacgc cggctcacat cggccgagat caggcagcgg atggtcgacc atttttcctt
     3001 ggataccctt gtattgaaca ctgaacgtga gttgctggct ctctgtggct cttctaccgg
     3061 aaatcggatt ccggcaagag aaaacccgac tatcggctga agagtcgatt ctcactcgtt
     3121 gtcgcgccga tgaactcaac cattcgaatt gtctatgcgg tctgtgcggt ggcggtgttc
     3181 tatggcaatg tccacaacta ccttcatgat caccatggaa tcggcgtacc gtggcactgg
     3241 gtcctcgcct tcatcgtttt ttcgcttccg gtgctgttca agcaggtaat gcagaccgac
     3301 ctgctgcggc aaccgattgt gctctggtgt cttggatatg cgtggttgac gatggtgtgg
     3361 tttctgtggg ggccgcagtc ggacatggct tggcaggagg tacgatggcg ggtgcttgcc
     3421 atcgttgaaa tgctgagttt cctggccgtg ttcatggatg cgcaagccga tcggtttgca
     3481 cgaatcacct tggtgatcgg ggtgcatgtc ggcattgcgc tcaatatcta tgagctgttc
     3541 gtgcccatgt cgttcagcaa tgtgttcggg cgctcggccg gtctctacct cagccccact
     3601 ttgaccaaaa tgtcgtcggg caaatacatt ttcatatcga cgtacatcat
//
LOCUS       DAORRQ010000160         3643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_700_length_3643_cov_9.921962, whole genome shotgun sequence.
ACCESSION   DAORRQ010000160 DAORRQ010000000
VERSION     DAORRQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_700_length_3643_cov_9.921962"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1283)
                     /locus_tag="PK224_21040"
     CDS             complement(258..1283)
                     /locus_tag="PK224_21040"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRB18290.1"
                     /translation="MTDSLPPSRRTAAEDAIRWFLRLREPGCSAEDTGLFEQWLASDP
                     AHHAEYLAVQATWKHVDAVATRPSPELQFHLAHAMTIQVKSQREHRGRILSQLTALAA
                     MLLLVSGTLWWWSFAVTESTYRTAKGRQQTVTLSDGTILDLNTESSVTVRMWGRGRQV
                     ILHTGEAYFTVAKDPARPFEVVALNGHIRDIGTQFSVYRQADRVLVAVESGAIDVVIP
                     STGNEPSQAHVLSPGERAAYTTAGEWSRLDQIDPRRIAAWRQGTLRFDRIPLIDAIRE
                     IERYWTGRILLADPALGSISVSGVFSHHNLAEFFTALPTIIPVQVTHRDGDIILTASM
                     MPSVRPQ"
     gene            complement(1318..1902)
                     /locus_tag="PK224_21045"
     CDS             complement(1318..1902)
                     /locus_tag="PK224_21045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRB18291.1"
                     /translation="MALVGQAPYDALGAQATEQGPASTMESNPTNRTLGAYTGYYREL
                     LSFLTAQLRCPHEAADLVQETFARLLALDNPDVVRQPRAFLYRIARNLAIDSSRKVSV
                     RNRFLIDLSELEEAPSAAPLPDYLLEGNQLRRALDQTITEMPPRRRDVFLLYRFAGLK
                     QAEIAERLGISTSMVERHLMKAMAQCRQRLQSHL"
     gene            complement(2100..2354)
                     /locus_tag="PK224_21050"
     CDS             complement(2100..2354)
                     /locus_tag="PK224_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18292.1"
                     /translation="MHYLALLVLLLSGCVEGRWVHPENTADQTAQDWEHCKAEVLSGQ
                     EHNKETLAGGINLSGCMHGKGYSYVEDRQPQAGADTSTHR"
     gene            complement(2463..>3643)
                     /locus_tag="PK224_21055"
     CDS             complement(2463..>3643)
                     /locus_tag="PK224_21055"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRB18293.1"
                     /translation="FLEPDERTFQRFQVLQPAVARRHANSMVTNLVGHIRFLEMDHTL
                     LTGVELRQEKTDQFIFTSGSAPSLDLFAPNYLLSPLPFDGDRVSFQADNKTAAFYVQD
                     QITLLPNLKIMGGLRFDYVHQSTQSTTESHASDNHAVSPRLGLVYQPIEPISLYTSWT
                     KGFQPSSPSSFNPNGNLFKPERSTQYEVGMKTFLFDNRVSATLAWFHLTRENLLTPNP
                     DPALALQGFSVQTGEQRSQGVELDVTAQITPGWNIITGYAYTDAEVTKDNDLSLVAKR
                     LANVPYNKFTLWSTYHFQEGPLQGFGFGGGLFAYSSRNSTIFEGQVDMPGYIRADAAL
                     YYNRDLQKGNWLGAKGVNVALNIRNLLDQRYVATSYNGSSQFFFGEPRTVLATVGLRF
                     "
BASE COUNT          706 a          998 c         1161 g          778 t
ORIGIN      
        1 aacgagagca ccgtatcttg agcatccgcc tgtacagcgg ccaggcctgt gatcgccagc
       61 acggccgaca agattcggac gagtgtgcga agccaccggc tcaccgtgac tcgcgtttct
      121 tgtacggcat cgcatttcct cacggtcatc atcgtgctcc cccgcgcagt tctgtggtgg
      181 tgaacgttct tcttgatctg tacagaagac gagtgagggg ggcggtcccc aacctgttca
      241 cgtgaaattg tcggggatta ctgcggacgc acggacggca tcatcgacgc agtgaggata
      301 atgtctccgt cgcgatgggt gacctgaacc gggatgatgg tcggtagcgc cgtaaaaaat
      361 tctgcaagat tatggtggct gaacaccccg ctcacggaga tgcttcccag cgcgggatcg
      421 gccaggagaa tccgtccagt ccagtagcgc tcgatttccc gtatggcgtc gatcaacggt
      481 atccggtcga accggagggt gccttgccgc caggctgcga ttcgtcgcgg gtcgatctga
      541 tcgagccgcg accactcgcc ggcggttgtg taggcggctc gctctccagg gctcaggaca
      601 tgggcttgtg aaggctcgtt gcctgtggag ggaatgacca cgtcgatcgc tccactctcg
      661 acggcaacca gcacgcgatc ggcctgccgg tagaccgaaa actgcgtgcc gatgtcgcgg
      721 atgtgcccgt tgagtgcgac gacttcgaac ggccgggccg gatctttggc gaccgtaaaa
      781 taggcctcgc cggtgtggag gatcacctgg cgtccccggc cccacattcg taccgtgacg
      841 ctgctctcgg tattgagatc caagatcgtg ccatcggaga gggtgacggt ctgttgcctg
      901 cctttggccg tgcggtatgt gctttcggtg accgcgaagg accaccacca cagggttccg
      961 ctcaccagaa ggagcatcgc cgccaacgct gtcaattgac tgaggatcct gcctcgatgt
     1021 tctctctgcg atttcacttg gatggtcatc gcgtgggcga ggtggaattg caattcggga
     1081 ctgggtcgag tcgcaacggc atccacgtgt ttccacgtcg cctgtaccgc caggtactct
     1141 gcgtggtggg cagggtcgga ggccagccat tgttcgaaga gccctgtgtc ctcggcagaa
     1201 cagcccggtt cacgcaggcg gagaaaccat cggatggcgt cctcggctgc agttcgtcgc
     1261 gatggaggca acgaatcggt catgaccgat caaggccttg ggctggtggt agagatgtca
     1321 caggtgtgac tgcaatcgtt gtctgcattg cgccatggct ttcatcaagt gtcgctccac
     1381 catcgaggtc gagatgccga gtcgttcggc aatctccgcc tgcttcaacc ctgcgaagcg
     1441 gtagagcaga aagacatccc gccggcgagg cggcatttca gtgatggtct gatccagtgc
     1501 gcgtctgagc tgattcccct cgagcagata atcgggcagc ggagcggcgg atggggcttc
     1561 ctccagttcg ctgagatcga tcaggaaacg attgcggacg gaaactttcc ggctcgagtc
     1621 gatggcgagg ttgcgggcaa tgcgatagag aaacgcccgt ggctggcgga ccacgtccgg
     1681 gttatcgagc gcgagaagtc tggcgaaggt ttcctgcacc aggtccgccg cttcgtgcgg
     1741 acaacgcagc tgggccgtca ggaagcttag aagttcccga tagtatccgg tgtatgcgcc
     1801 cagtgtgcga ttggtcggat tggattccat ggttgaggca ggtccttgct cggttgcctg
     1861 agcgccgagc gcatcataag gggcctggcc cacaagggcc atgtctacac tcctgaatgt
     1921 tgtgcgttgt tcctggaaaa gggtgacggg cgagagctga aaaacccaac ctccaggcag
     1981 gaagcagagg gtctttctgc tcggcccatt tggcgatgag gagagaggct gtatctgaag
     2041 aggaacgacc gatcgggagt gccaggcggt ggcgtatccg ttcgtgcaga ccgatgccgt
     2101 cagcgatgcg tcgaggtgtc ggctccggcc tgcggctggc ggtcttcaac gtaggaatac
     2161 cctttgccat gcatgcagcc gctgaggttg atgccgcctg ccagggtttc tttgttatgt
     2221 tcctgccccg agagcacctc cgccttgcag tgttcccagt cctgtgcggt ttggtctgcg
     2281 gtattctccg gatgaaccca tcggccttcg acgcagccgg acaggaggag gaccaggagt
     2341 gcgaggtaat gcatagaatt ccttttcaga gaatcgtccg ttgtttcccc ggcgatggca
     2401 ggtaaggcgg accacgggat aacaccggtg gcccgccttc tgtattccgt accgggacga
     2461 ggtcaaaacc gcagccccac ggtcgctagc accgtccggg gttcgccgaa gaagaactgc
     2521 gacgagccgt tgtaagaggt ggcgacgtat cgctgatcca gaaggttgcg gatattgagt
     2581 gcgacgttca ctcctttcgc tcccagccag ttgcccttct gcagatcccg attgtagtag
     2641 agggctgcat cggctctgat gtagccgggc atgtccactt gcccttcgaa aatcgtggaa
     2701 ttgcgactgc tataggcgaa cagccctccg ccgaatccga atccctgcag cggaccttcc
     2761 tgaaaatggt acgtcgacca gagggtgaac ttgttgtagg gcacgttggc cagtcgtttc
     2821 gccaccagtg acagatcgtt atccttggtc acctcggcgt cggtataggc atagccggtg
     2881 atgatgttcc accccggggt gatttgggcg gtcacgtcca attccacgcc ctgactgcgt
     2941 tgttcacccg tctgaaccga gaatccttgc agcgccagcg ccggatcagg gttgggcgtg
     3001 agtagatttt cacgggttaa gtggaaccag gcgagggtag ccgacacgcg gttgtcgaag
     3061 aggaacgttt tcataccgac ctcgtattgg gtcgaccgtt ccggcttgaa caggttaccg
     3121 ttcgggttga aggaggaagg ggagctcggc tgaaacccct tggtccagga tgtatagagc
     3181 gaaatgggtt cgatcggctg gtagaccagg cccaatcgag ggctcaccgc gtggttatcg
     3241 gatgcatgac tctccgtggt ggactgtgtg gactgatgta catagtcgaa acgcaggccg
     3301 cccataattt tgaggttcgg cagaagggtg atctgatcct gcacatagaa agccgcggtc
     3361 ttgttgtcgg cctggaaact gactcggtct ccgtcgaacg gcagcggact gagtaagtag
     3421 ttcggcgcga agagatctag tgacggagcc gaacccgacg tgaagatgaa ttgatcggtc
     3481 ttctcctgcc gcaattcgac accggtcaac agcgtatggt ccatctccag aaagcgaata
     3541 tggcctacca ggttggtcac catcgaattg gcgtgccggc gggccacggc cggttgcagg
     3601 acctgaaatc tctggaaggt gcgctcgtcc ggttccagaa aga
//
LOCUS       DAORRQ010000161         3633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_702_length_3633_cov_6.444941, whole genome shotgun sequence.
ACCESSION   DAORRQ010000161 DAORRQ010000000
VERSION     DAORRQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_702_length_3633_cov_6.444941"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PK224_21060"
     CDS             <1..691
                     /locus_tag="PK224_21060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRB18294.1"
                     /translation="LCAAKYRLSRQDVDDFALESYSRARDAIARGAFKKEIVPVEVPQ
                     KKGASVLVSDDEEPNRVDLTKLRQLKPVFQDDGVLTVGNSPSCNDGAAALVIMGEEEA
                     ARLGLAPMARIVGYAGAALAPEWFTVAPVEAIRRLLKQTGLSIGDIDLFEINEAFSAV
                     SLAINRELGLDPKKVNVNGGAVALGHPIGATGARIVTTLLYAMEARDAHRGLAALCIG
                     GGEALALIVER"
     gene            705..1586
                     /locus_tag="PK224_21065"
     CDS             705..1586
                     /locus_tag="PK224_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRB18295.1"
                     /translation="MTIDDIKTIGVVGAGQMGRGIAQVLATAGWNVLLIDATEEALED
                     AIKKIWQGVTKAVEKGALRGDQAGSAMAIIHPMKHMQRLREAQVVIEAIPEDPVMKRA
                     LFAQLGQVCEPSTLLASNTSSISIASLGMASGRPDRVVGIHFMNPVPVMRLVEVVRAI
                     ETSEHTVQLALELVRRAGKTAVVCKDSPGFIVNRVLIPMINEAIFALEEGVATAEAID
                     LAMVEGTNHPVGPLALADRIGLDTVLAICEVLHQDLGDPKFRPCPLIRKYVEAGRLGR
                     KSGHGFYVYGDQPVTVS"
     gene            1617..3212
                     /locus_tag="PK224_21070"
     CDS             1617..3212
                     /locus_tag="PK224_21070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRB18296.1"
                     /translation="MNERKSRFSSLSGLDIERVYSSDHLKDWNVERDLGQPGSFPYTR
                     GIYPSMYRSRLWTMRQFAGFGSADDTNRRFKYLLAQGQTGLSVAFDLPTLMGLDADDP
                     MARGEVGHCGVAISSLADMERLFDGIPLDQVTTSMTINGPAAVLFAMYLAVAEKRGIP
                     FDRLGGTIQNDILKEYIAQKEWLFPPEPHIAIIVDTIAYCAAQVPKWHPISISGYHIR
                     EAGSTAVQELAFTLYDGLTYVDAAVKSGLDVDAFAPHLSFFFNVHNDFFEEIAKFRAA
                     RRMWAREMEQRYHPKNPRSLQLRCHAQTAGCSLTAQQPMNNVVRTTLQALAAVLGGTQ
                     SLHTNSMDETLALPTEEAVTLALRTQQVIASESETVNCVDPLGGSYFVESLTNRLEEG
                     ARDYFRTLDRMGGMVAAIEQGFPQREILDASQWYQREIEHEERPIVGVTDYVEQETRS
                     IPILKIGPEVEQEQVARLSDLRKSRDPFKMAGSLEELQETAACHQNLLPALIDAVKAK
                     ATLGEICAALKEVHGTYREPVVL"
     gene            3209..>3633
                     /locus_tag="PK224_21075"
     CDS             3209..>3633
                     /locus_tag="PK224_21075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB18297.1"
                     /translation="MKLGAFDIYAVTDGRFRLDGGAMFGVVPKALWQKCCPADELNRI
                     PLHLNCLLIQAHGKNVLVDTGLGDKEDAKFHSLFAVERMPSLRDSLQRHGLTHDDIHL
                     VINTHLHFDHAGGNTARNGNGLLQSAFPKATYYVQQGEY"
BASE COUNT          769 a         1086 c         1092 g          686 t
ORIGIN      
        1 gttgtgcgcg gccaagtatc gattgtcgag acaagacgtc gacgacttcg ccctggaaag
       61 ttacagccgg gcccgtgacg cgatcgcccg cggcgccttc aaaaaagaga tcgttccggt
      121 cgaagtaccg cagaaaaagg gagcttcggt cctcgtctcg gacgacgagg aaccgaatcg
      181 ggtggatctg accaagttgc gccagctcaa accggtgttt caggacgatg gagtcctgac
      241 ggtgggcaac tccccctcct gcaacgatgg agcggcagca ttggtcatca tgggtgaaga
      301 agaagccgcc cgccttggcc tcgcaccgat ggctcgcatc gtcgggtatg ccggtgccgc
      361 gctggcgccg gaatggttca cggttgcgcc cgtggaagca atccggcggc tgctgaaaca
      421 gaccggactt tccatcggtg acatcgatct gttcgagatc aatgaagcgt tttcggccgt
      481 ctccctggcg atcaatcgtg aactcggcct cgatcccaag aaggttaacg tcaacggcgg
      541 cgcggtggcc ctgggtcatc ccatcggcgc caccggagcc aggatcgtaa cgacgctcct
      601 gtatgccatg gaggcgcgcg acgcccatcg agggctggcc gccctctgca tcggaggcgg
      661 agaagcgctc gccctcatcg tggagcggtg aaccagggat cgttatgacg atcgacgaca
      721 tcaaaaccat cggggtcgta ggcgcggggc agatggggcg cggcatcgcg caggtgctgg
      781 ccacggcagg atggaatgtc ctcctcatag atgcaacgga agaggcgctg gaagacgcca
      841 tcaagaaaat ctggcagggg gtcacaaaag cggtcgagaa aggcgccctg cgtggagatc
      901 aggccggatc ggccatggcg atcatacatc cgatgaagca catgcaacga ttacgagagg
      961 ctcaagtggt gatcgaagcc atacccgaag atcccgtcat gaaacgagcg ctgttcgcgc
     1021 aattagggca ggtctgcgaa ccatcgaccc tcctggccag caatacgtcc tcgatttcga
     1081 tcgcgagtct gggcatggcc tcgggtcgtc ccgatcgcgt cgtcgggatc cattttatga
     1141 atcccgtccc ggtcatgcga ctggtcgaag tggtgcgcgc catcgagacc tctgagcata
     1201 cggtgcaact ggccctggaa ctcgttcgtc gagcagggaa aaccgcggtg gtctgcaagg
     1261 attcccccgg cttcatcgtc aaccgggttc tcattcccat gattaacgag gccatttttg
     1321 cgctggagga gggagtggca acggccgaag cgattgatct ggcgatggtg gaagggacca
     1381 accatcccgt gggtcccctg gccttggccg accgcatcgg cctggatacg gtgctcgcaa
     1441 tttgcgaggt gctgcatcag gacctgggag acccgaagtt tcgcccctgt cccctgatac
     1501 gcaagtatgt cgaagccggt cggctgggga gaaagagcgg acatggattc tatgtgtatg
     1561 gagaccagcc ggtgactgtg agctgacgtt ctcgttcctt cccggagggc cgcatcatga
     1621 acgagcggaa atctcgcttc tcctccctct ctggattaga catcgaacgg gtctacagct
     1681 ctgaccatct gaaggattgg aacgtcgagc gggatctcgg ccaaccgggc tcattcccct
     1741 acacccgcgg catctacccg agcatgtacc gcagccgcct ctggaccatg cggcaattcg
     1801 cgggtttcgg ctccgcggac gatacgaatc ggcgcttcaa gtatctcctg gcacagggcc
     1861 agaccggcct cagtgtcgcc ttcgacctgc cgacgctcat gggccttgat gccgatgatc
     1921 cgatggcacg gggcgaagtg ggtcactgcg gcgtggccat ctcctcactc gccgacatgg
     1981 aacggctgtt cgacggcatc ccgctcgacc aggtcaccac ctcgatgacg atcaacggac
     2041 cggccgcggt actctttgcc atgtatctgg ccgtggcaga aaaacgcggc atcccgttcg
     2101 accgactcgg cggcacgatt caaaacgata ttctgaaaga atatatcgcg caaaaggagt
     2161 ggctcttccc gcccgaaccg cacatcgcga tcatcgtcga tacaatcgcc tactgtgccg
     2221 ctcaggtccc caagtggcat ccgatcagca tcagcgggta ccacatccgg gaggcaggct
     2281 cgacagccgt tcaagaactc gcgttcacac tgtacgacgg actcacctat gtggatgcgg
     2341 ccgtgaagtc cggcctcgac gtggatgcct tcgcgccgca cctctctttc ttcttcaacg
     2401 tgcacaatga cttcttcgaa gagattgcca agttccgggc cgcccggcgt atgtgggcgc
     2461 gtgaaatgga gcaacgttac cacccgaaga atccccgctc cctccaactc cgctgtcacg
     2521 cccagacggc cggctgctcc ctgaccgccc aacaaccgat gaacaacgtg gtgcgcacca
     2581 ccctccaggc cctggccgca gtgctcggcg ggacccaatc gctccacacc aactcgatgg
     2641 atgaaaccct ggcgctcccc acagaagaag cggtgacgct ggccttgcgg acacaacagg
     2701 tcatcgcctc cgagagtgaa accgtcaact gcgtcgatcc gttgggcggc tcctactttg
     2761 tcgaatcgct gacaaaccga ttggaagaag gagcgcggga ctatttccga acactggatc
     2821 ggatgggtgg aatggtggcg gcaatcgaac agggatttcc gcagcgggaa atcctcgatg
     2881 cgtcacaatg gtatcaaaga gaaattgaac acgaggagcg gcccatcgtc ggcgtcaccg
     2941 attatgtgga acaggaaaca cgttccatcc caatcttgaa gatcggaccg gaagtggaac
     3001 aggaacaggt cgcgcgcttg tccgatctca gaaaatctcg tgatcccttc aagatggccg
     3061 gctcattaga ggagttacag gaaacggccg cctgccacca gaacctcctg cccgctctga
     3121 ttgatgccgt gaaggcgaaa gccacattgg gagagatctg cgccgcgttg aaggaagtgc
     3181 atggaaccta tcgcgagccg gtggtgttat gaaactcggt gcgtttgaca tctatgcggt
     3241 gaccgacggc cggttccggc tggatggtgg tgccatgttc ggggtggtgc cgaaggcgct
     3301 ctggcagaaa tgctgcccgg cggatgagct gaatcgtatt ccgcttcact tgaactgtct
     3361 gctgatccag gcccacggca agaacgtcct ggtggatact ggtctcggcg ataaggagga
     3421 tgccaagttc cactccctgt ttgcagtcga gcggatgccg tccctgcgcg actcgctgca
     3481 gcgccatggc ctcacccacg acgacattca tctggtgatc aacacccatc tgcacttcga
     3541 tcatgccgga ggcaatacgg ccaggaacgg gaacggcctg ctgcagtcag cctttcccaa
     3601 ggccacctac tatgtgcaac agggcgagta cga
//
LOCUS       DAORRQ010000162         3592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_709_length_3592_cov_7.906135, whole genome shotgun sequence.
ACCESSION   DAORRQ010000162 DAORRQ010000000
VERSION     DAORRQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_3592_cov_7.906135"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PK224_21080"
     CDS             <1..660
                     /locus_tag="PK224_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB18298.1"
                     /translation="ASLPSFPTPQLPAPYKPEEPMPPAQASVSLQGVFRHLDTVLHEK
                     TLVIADVGESLFASVDLHVHRRFEFLSPAYYTSMGFAVPAAIGAGFADPSLRPIVLVG
                     DGAFQMTGSELSTAVRYKQAPVVIVLNNHGYSTEREILEGPFNDIHEWRYERICELIG
                     GGQGSRVATHGEFVQTLGKALADPSQPHVINVLLDPADRSPAMMRLARRLAKRLSTDR
                     P"
     gene            854..1816
                     /locus_tag="PK224_21085"
     CDS             854..1816
                     /locus_tag="PK224_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18299.1"
                     /translation="MSTKLPDGKTMRDGQIFSARLNNVVTTEREFDELVAKSLPELLD
                     RATGQTKRFLRETNQWGDDITHEKLALRWGYELVERFVVFGRTEVPCRPFFLLDSLIA
                     KSFSQPDPLCYHKELLTPVGRFLDGLASRAVVSRDALIALFYHFYGFSQAHVVKLLGF
                     GQAESQRVYKNFERWRQTGWQRTMNETGLSGSEIEVLEQELRTRPDHLNHEVDRLMPV
                     LQSHYRKSEPEHYPCLSEQKWGQLFHDDYGHDYRVWHLAFCRDCFLEVADRRQAEVSS
                     GLKPQVNLHIHPLKKGGVLALFGGDRGGRHHGNTRAQRLSRTSA"
     gene            1773..3068
                     /locus_tag="PK224_21090"
     CDS             1773..3068
                     /locus_tag="PK224_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18300.1"
                     /translation="METPELNDYRAHLLDALDDQPRRAGEERPEFLQVLVNHERVGTL
                     NCSHTDPSFAYTARERSIQHLEIRNESGVLVGGCVAPEAGQKSVRVPVRKGSVAIQVQ
                     NHFDGGSLRLQYAAAPGWWSHLATSIGLSSATTAPVSSRTPVWTMTTALAQIVLAVGV
                     VVLLAERIPTWLNAEDRAHQLEEELAARQSTQEQIDRVHQQLAQLVEAQASAVAVSRS
                     EQDRIAQLNALLDTVAHAQQKLNTQVVSVQEDLKTVKTDVAQEVETGLRVAVNAVEAD
                     RDLVRQDLRSIKSVNETLIKQVALLESRNRELHARLALTSLEVAKANAAPKPTVVAKG
                     DLPKEAPMATPVADNIREADRQAFMFWVAFQDGTTEKSIEDLVQELNGIKKGPMKSGW
                     YSVEVNLPNPEPPDRFLESVKRAKIVKSVVTSKAMPPAQ"
     gene            3199..>3592
                     /locus_tag="PK224_21095"
     CDS             3199..>3592
                     /locus_tag="PK224_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRB18301.1"
                     /translation="MSDFHQNGLVTVLHRLGASNLEQLEKELERHAASNPIALVLPSL
                     YSELENPALKHIVQVLKDIRYVNEIVISLDRASALEFRLAKQFFSVLPQRVRIVWNDG
                     AGIQDILKLLANTEIDTGLQGKGRGCWMA"
BASE COUNT          746 a         1105 c         1057 g          684 t
ORIGIN      
        1 gcctcactcc cttcatttcc aaccccgcaa ttgccggctc cgtacaagcc cgaagagccc
       61 atgccgccgg cgcaggcgtc cgtcagtctg cagggagtgt ttcgtcacct cgataccgtg
      121 ctccatgaga aaaccctcgt cattgccgac gtgggcgaat cactctttgc ttccgtcgat
      181 ctccacgtac accgccgctt cgaatttttg tcccccgcct actacacatc aatgggattc
      241 gcggtcccgg ccgccatcgg cgccggtttc gccgacccgt cgttgcggcc gatcgtgctg
      301 gtgggagacg gcgcgtttca aatgaccggg tcggagctct cgactgccgt gcgctacaaa
      361 caagcaccgg tcgtgatcgt cctgaacaac cacggctatt cgaccgagcg cgaaattctg
      421 gaaggcccct tcaacgacat ccacgaatgg cgttatgaac ggatctgcga gctgatcggc
      481 ggcggtcagg gctcccgggt ggccacccac ggggaattcg tccagaccct gggcaaggca
      541 ctcgcagatc ccagtcaacc ccatgtcatc aatgtgctgc tcgatccggc cgaccgctcg
      601 ccggccatga tgcgcctggc gcggcggctg gcgaagcggc tctcgaccga ccgcccctaa
      661 cccctgtgcg tatcacgatt cttttgctcc gcaggccctc cgacgagagg gccccgtacc
      721 ccacacccag acacctgttt cacgctgata cagtgcggcg ttgcggccac cgcacccatt
      781 gaccctccgt ggacaaatgt ggtcttctag gggcgtactt gtcgaattgt catttgacgg
      841 ggaattcgcc actatgtcga cgaagctgcc ggatggaaag accatgcgag acggtcagat
      901 cttctctgca cgactgaata atgtcgtcac cacggaacga gagttcgacg agttggtcgc
      961 taagtcgctg ccggaattgt tggatcgcgc cacgggtcaa accaagcgct ttctccgtga
     1021 aaccaatcaa tggggcgacg acatcacgca tgagaagctg gcgctgcgct gggggtatga
     1081 gctggtggag cggttcgtgg tcttcggtcg taccgaagtg ccctgccgtc ccttcttcct
     1141 cctggatagc ctgatcgcca aatcgttcag tcagccggac ccgctctgtt atcacaagga
     1201 gctcctgacg ccggtgggac gctttctcga tgggctggcc tctcgcgctg tggtcagccg
     1261 cgacgccctg atcgccctgt tctatcactt ctatggcttc agtcaggccc atgtggtgaa
     1321 gttgttaggg ttcggacagg ccgaaagcca gcgggtctat aagaacttcg agcggtggcg
     1381 ccaaacgggg tggcaacgca cgatgaacga aaccgggtta agtggatcgg aaatcgaagt
     1441 actggagcag gaattacgca ccagacccga ccacctgaac catgaagtgg accggctgat
     1501 gccggtcttg cagtcgcatt atcgaaagag cgaaccggag cattacccct gcctgtctga
     1561 acagaagtgg ggtcaactat ttcacgatga ctacggccac gactaccgcg tctggcattt
     1621 ggccttttgc cgcgactgct tcctggaagt cgcagatcgc cggcaagccg aggtcagcag
     1681 cgggttgaag ccgcaggtca atctccatat acatccgctg aaaaagggag gcgtcttggc
     1741 tctctttgga ggggatcggg ggggacggca ccatggaaac accagagctc aacgactatc
     1801 gcgcacatct gcttgacgcc ctggacgatc aaccgcggcg cgcgggtgag gaacgtccgg
     1861 aattcctgca ggtactggtc aaccacgaac gcgtcgggac gctgaattgc tcccacaccg
     1921 atccgtcctt cgcctacacg gcccgcgagc ggagtattca acacctcgag atccggaacg
     1981 agtccggtgt gctggtcgga ggctgcgtcg ctcccgaagc cggccagaaa agtgttcgcg
     2041 ttcccgtcag aaaaggcagc gtcgccattc aggtccaaaa ccatttcgac ggcgggtcgc
     2101 ttcgactcca gtacgcggcc gcccccggat ggtggtctca tctggcaacg tcgatcggac
     2161 tctcctcagc aaccaccgca cccgtttcaa gcagaactcc cgtctggacc atgacgacgg
     2221 ccctcgcaca gatcgtgctg gccgtgggag tcgtggtgct gttggctgaa cggattccaa
     2281 cctggctcaa cgctgaagac cgggcgcatc agcttgaaga agagctggcg gccaggcaat
     2341 ccacacagga gcagattgat cgtgtgcacc agcaactggc gcaactggtt gaggcgcagg
     2401 cgtcagccgt ggcggtgtcc cggtcggagc aggaccggat cgcccagctg aatgccctcc
     2461 tcgataccgt cgcccatgca caacagaaac tcaacacaca agtcgtctcc gtccaggaag
     2521 acctcaagac agtcaagacc gatgtcgccc aggaagtcga aaccggcctg cgcgtcgcgg
     2581 tcaatgcggt ggaagccgac cgcgacctcg tgcggcagga tctgaggtcg atcaagtcgg
     2641 tcaatgaaac cctgatcaag caggtcgccc tgctggaaag ccgcaatcgg gagttacatg
     2701 cccggctggc gctcacctcg ctcgaagtcg ccaaagccaa cgcggcgccc aaaccgaccg
     2761 tcgtggccaa gggagacctg ccgaaggaag cgcccatggc gaccccggtc gcggataaca
     2821 tccgggaggc agaccgtcag gcctttatgt tctgggtggc gtttcaagac ggcacgaccg
     2881 agaagagcat tgaggatctg gtccaggaac tgaacggcat caaaaaggga ccgatgaagt
     2941 cgggctggta ttccgttgaa gtgaacttgc cgaatccgga acctccggac cgattcctgg
     3001 agtctgtgaa gcgagccaag atcgtgaagt cggtcgtgac cagcaaggcg atgcctccgg
     3061 ctcagtagtc gcgatctccc ctcagcaaat tcaaccaccc ggcacggtga gtgtccgcct
     3121 ctcgtgtgga gggcgcctcc ctgccgcact ggtcatttgc tttctatcat ccgctccaaa
     3181 cccgacacag gagggaatat gtcggatttt catcagaacg ggctcgtgac ggtcttgcac
     3241 cgtctgggcg cctccaatct tgagcaactc gaaaaggaac tggaacgaca cgcggcgagc
     3301 aacccgattg ccctcgtcct gccttcgctg tattcggaac ttgagaaccc cgcgctgaag
     3361 cacatcgtgc aggtgctgaa agacatccgg tacgtcaatg aaatcgtgat ctctctggat
     3421 cgcgcctcag ctctggagtt ccggctggct aaacaattct tttcggtgct gccgcagcgg
     3481 gtccggatcg tgtggaacga cggggcaggg attcaggaca tcctcaagct actggcgaac
     3541 actgaaatcg acaccgggct gcaaggcaag ggccggggct gttggatggc at
//
LOCUS       DAORRQ010000163         3519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_721_length_3519_cov_103.547633, whole genome shotgun sequence.
ACCESSION   DAORRQ010000163 DAORRQ010000000
VERSION     DAORRQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_721_length_3519_cov_103.547633"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PK224_21100"
     CDS             complement(<1..416)
                     /locus_tag="PK224_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18302.1"
                     /translation="MKTSITYTKTQDGSQATVLSLLTVLVIVVGLLLEFPLMPAAAEL
                     KTDFGVYPEPPLQPLSQAGAKISDPVFGTRILRVTDAADGNTGAGTLYSYYPAFNANN
                     TYLAVRETIGYARAKFFRFDAVNFTAGPGFILSSPPA"
     gene            839..1972
                     /locus_tag="PK224_21105"
     CDS             839..1972
                     /locus_tag="PK224_21105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB18303.1"
                     /translation="MMLYRLMSRLDRTKFCPQVISMMELGPVGEKILALGIPVRSLGM
                     QQGIPNPLAVFRLVDWLKRDKPDVIQTWMYHADLLGSVAAKLVGGIAISWNIRHSDLS
                     GEESKRLTHVTARLCAQLSRWAPQKIVCCSESAREVHTALGYAADKMVVIPNGYDLET
                     FRSDPAARRRIRLELQVPESAPVIGLVGRFDPQKDHRTFLRAAGLLHQKRPNVHFILC
                     GESVTRQNASLMKWIDEAGIQNHCHVLGRRDDIPQITASFDIASLSSSFGEAFPNVVS
                     EAMSCEVPCVVTDVGDAALIVGESGMVVPTRNPTALADAWRTMIDLEPDMRTRLGSAA
                     RQRVMDRYNLSEIVSRYESLYEELACTGRPQDYLSSRRRYVRD"
     gene            1959..>3519
                     /gene="asnB"
                     /locus_tag="PK224_21110"
     CDS             1959..>3519
                     /gene="asnB"
                     /locus_tag="PK224_21110"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRB18304.1"
                     /translation="MCGISGFVDTGRRQDDRELRATVLRMANAIRHRGPDDVGTWEDA
                     AAGVALGHRRLSILDLSSLGHQPMRSACGRYVTSFNGEIYNFRALREELEDRGHSFRG
                     HSDTEVMLAGLSQWGLDSAVRRFNGMFAIAVWDRETHQLHLVRDRMGEKPLYYGWAGK
                     TFLFASELKAIRAHPIFEGVIDRGAVALYMRHNYIPAPYSIYSGISKVIPGSIVTITP
                     DPAAVPQVRPYWSVAAAVEQGSADPFSGTANDAIDDLEKLLRDAVKLRMEADVPLGAF
                     LSGGVDSSTIVALMQAQSTRPVQTFSIGFHETAYNEAHHAKAVADYLGTAHTELYVSP
                     AEAMEVIPRLPTLYDEPFADSSQIPTYLVSELTRRHVKVALSGDAGDELFAGYNQYRW
                     GLRFWNHAAGIPWSARKLVAKAVTRISPRSWEVSLRMTAPLLPPRLRQRDMGHKLHTL
                     ADMFAAECPEAMYRTIVSHWHAPASLVIGASEPQTVLTDRAQWADLSDVMQRMMYVDM
                     KMYLPDDILVKV"
BASE COUNT          739 a          929 c         1030 g          821 t
ORIGIN      
        1 gccggtggag aactcaggat aaagcccgga ccagccgtga aattgacggc atcgaatcga
       61 aaaaacttgg ccctggcata accgatcgtt tcccgcactg caaggtaggt attgtttgcg
      121 ttgaatgcag gataatagga gtacaaggtc cccgctccgg tatttccgtc cgctgcgtca
      181 gtgacacgaa gaattctcgt tccaaaaacc ggatcggaga tctttgctcc ggcctggctc
      241 agtggctgaa ggggaggctc gggatagaca ccgaagtcgg tttttagttc tgcggcggca
      301 ggcatcaggg gaaactcgag aagtagtcca actacgatca ctagtacagt gaggagagac
      361 agaactgtcg cctgcgatcc atcctgcgtt ttggtatagg tgattgatgt cttcattgtt
      421 ccctccgatg tacgcttgaa catatgagca atgagcatgc caagcgaatc taggggttat
      481 ttcccgaaga ctatccttta tgtggttgtt tttgcatcat cccgtttacg gtatctttca
      541 ggcccactat tgtttcgtcc aaccttggac gttgtgtaga tcacagcata catagctccc
      601 ggcttatttc ctatgatgcc ggattctaag acccaaggcg taacggggag aggcgtccgg
      661 ggattcaacg gcgttttctc tcagggtgaa ctctagcctc tgggaatcca atagcaccga
      721 tcagcaatcc gagtcacctg aaacaatcga aggatcagag acgttgtcct cctctagaaa
      781 caagccaccg gtatccgtgc tctgcctcat cacagccctg acgggaggag gggcggagat
      841 gatgttgtat cggctgatgt cccgtctgga tcgtacaaag ttttgtcccc aggttatttc
      901 catgatggag cttggaccgg tgggtgagaa gatcctggcg ttgggaattc ctgtccggtc
      961 gctggggatg caacaaggta taccgaaccc gctcgcggtg ttcagactgg ttgactggct
     1021 caagcgtgac aagccggacg tcatccaaac atggatgtac cacgccgacc tcctgggtag
     1081 cgtggccgca aaactcgtcg gaggcatcgc catttcgtgg aatattcgtc atagcgatct
     1141 cagcggtgag gaatccaaac gactgacaca tgtcaccgct agactctgtg ctcaactctc
     1201 gcgctgggct ccccaaaaaa ttgtttgttg ttcggaatca gctcgcgagg tgcataccgc
     1261 acttggatat gctgccgaca agatggtggt cattcccaat ggatatgatc tcgagacgtt
     1321 cagatcggat cccgctgcac gcaggcgtat tcgcctggag ttacaagtgc ctgagtccgc
     1381 accggtgatt ggactcgtgg ggcgctttga cccccaaaaa gatcaccgca cgtttctcag
     1441 ggcagccggc ctcctccacc agaagagacc gaacgttcat ttcatcctct gcggggagtc
     1501 ggtcactcgt caaaacgcga gtctgatgaa gtggatcgac gaagcaggta ttcaaaacca
     1561 ttgccatgtt ctgggacggc gagacgacat cccacagatt accgcttcgt tcgacattgc
     1621 ctcgctttcg tcttccttcg gcgaggcatt tccgaacgta gtcagtgagg cgatgagttg
     1681 cgaagttccc tgtgtcgtca ccgatgttgg tgatgccgca ttgattgtcg gcgagtccgg
     1741 catggttgtt ccgacgagga atccaaccgc cctagcggat gcttggagga ccatgattga
     1801 cttggagcca gacatgcgca cgaggcttgg atcggctgcc cggcagcggg tcatggatcg
     1861 atacaatctg tccgagattg tttctcgata tgagagtctc tatgaggaat tggcctgcac
     1921 aggccggcct caggactatc tgtcttccag gaggcgctat gtgcgggatt agcggctttg
     1981 tggatacggg gcgacggcag gacgatcgtg aactccgggc gacggtcctt cgaatggcca
     2041 atgcgatacg ccatcgtgga ccggatgatg tggggacctg ggaggatgcc gcggcaggag
     2101 tggccctcgg ccaccgccga ctttcgattc tggatctttc ttccttgggg catcagccaa
     2161 tgcggtccgc gtgcggtcgc tatgtgacga gcttcaacgg cgagatttac aactttagag
     2221 ctctccgtga ggaactggaa gatagggggc acagtttccg aggacactcg gatactgagg
     2281 tcatgctggc cggcctttcg cagtgggggc tggattcggc tgtccggcgc ttcaatggaa
     2341 tgtttgctat cgcggtgtgg gatcgagaga cacaccaact tcatttggtg cgcgaccgca
     2401 tgggggagaa gccgctttat tacgggtggg ccggcaagac cttcctcttc gcctccgaac
     2461 tgaaggcaat ccgtgcccat ccgatttttg aaggggtgat cgaccgcggg gctgttgcgc
     2521 tctacatgcg gcacaattac attcccgcgc catactcgat ctacagcggt atttcaaaag
     2581 tgatacctgg ctcaatcgtc accatcacac ccgatccggc tgctgtcccg caggtccgtc
     2641 cttactggtc ggttgcggca gcggtggaac aaggcagcgc cgacccgttc agtggaacgg
     2701 cgaacgatgc gatcgacgat ttggagaaac tcctgcggga tgcggtgaag ctacgaatgg
     2761 aggcagatgt gccgttgggg gccttccttt cgggcggggt ggattcctcg acgattgtgg
     2821 cactcatgca ggcgcaaagc actcgtccgg ttcagacatt ttccattggg ttccacgaaa
     2881 cggcctacaa tgaggcccat catgccaagg cagtcgctga ctatctagga accgcccata
     2941 ccgagttgta tgtctcgccg gcggaagcga tggaggtcat ccccaggctt cccacgctct
     3001 atgatgagcc atttgccgac tcttcgcaga ttccgactta tctggtttcc gagttgacaa
     3061 ggcggcatgt caaggtcgcc ttatccggag atgccgggga cgagctcttt gccggataca
     3121 atcaatatcg ctggggattg cggttctgga atcacgcagc aggaataccc tggagcgcac
     3181 gaaagctcgt tgcgaaggcg gtgacccgta tttctccgcg atcatgggag gtctctctcc
     3241 gcatgactgc tccgcttctt ccgccccgct tgcgacagcg ggatatgggg cacaaactcc
     3301 atacccttgc cgacatgttt gctgcggaat gtcctgaagc gatgtaccgg acgatcgtgt
     3361 cgcattggca cgctccggcc tcgctggtta tcggagcctc ggagccccag acggtcttga
     3421 ccgatcgggc acagtgggct gatttatcgg atgtgatgca gcggatgatg tacgtcgata
     3481 tgaaaatgta tttgcccgac gacattttgg tcaaagtgg
//
LOCUS       DAORRQ010000164         3497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_723_length_3497_cov_13.777745, whole genome shotgun sequence.
ACCESSION   DAORRQ010000164 DAORRQ010000000
VERSION     DAORRQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_3497_cov_13.777745"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK224_21115"
     CDS             <1..334
                     /locus_tag="PK224_21115"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HRB18305.1"
                     /translation="DAEQSYPALDIVSSVPAPFNIAFVASTIKTLTTGKPLFKGTASI
                     EGKLMDAESGEILAAAVDRRVGGRFLDAEVFDSWNDVHGALKYWSQLTRFRLCQLRKQ
                     TNCVSPGS"
     gene            579..743
                     /locus_tag="PK224_21120"
     CDS             579..743
                     /locus_tag="PK224_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18306.1"
                     /translation="MAQITEVAPDLFRITTFLEPFNLQFSQFLVRDAEPLLYHTGPRA
                     LFPAVKETVA"
     gene            762..1307
                     /locus_tag="PK224_21125"
     CDS             762..1307
                     /locus_tag="PK224_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB18307.1"
                     /translation="MRWIGFSHFEADECESLPEWQQLAPQSDAVCSLVSKLVSVDDCL
                     ALRPAKGMTDGEVLETGKYRFRFLATPHVPHCWESGLMFEETQRTLLCSDLLHQNGNV
                     EPVTQSDVVGRSRDVLVEYQQGPLANYMPYCTLTDPTLQRLAALQPKTVATMHGSLYV
                     GDGGKALRDLATVFREVLGAA"
     gene            1431..2078
                     /locus_tag="PK224_21130"
     CDS             1431..2078
                     /locus_tag="PK224_21130"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRB18308.1"
                     /translation="MRIRYVVIAFSLAGCLTIPGWGVIRAEPSFDQTAGYILNIVRAF
                     RTAYVLHVVEHARDAGLSPREDWKTDAHFLPLPAQFVKEAAEQVEGLEIGLISLTPLN
                     PANRPRTDAEMTALLQLEKDRQRGVIGFVDGDEFKAVSADLALVRSCVDCHNQHPRAV
                     RKNFQQWDVMGALVVRLKRTVEGEGQALPPEPPKRAPGLLEGPPPQPTITPPWVR"
     gene            complement(2175..>3497)
                     /locus_tag="PK224_21135"
     CDS             complement(2175..>3497)
                     /locus_tag="PK224_21135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRB18309.1"
                     /translation="LEACLAQARRSERRLRAQYAVTRVLAESSTLKDAGHEILRAIGE
                     SLDWELGMFWAVDKQAELLRFVDLWHAPQVEASEFIQDSRERTFQRGVGLIGRTWESG
                     TPIWIPDVTSDPHFRRASIAAHVGLHGGFAFPVRKGERIDGVIEFFSHQIRKPDQDIL
                     DMVADIGIKVGQFVDREQTAEALRQAEALADVARLLGDIGHDIKNMLMPIMSGAALLE
                     EELDECYHRLPESVTGNLKHSRDLTKELLDMIRNGSRRIQDRVREMAESVKGLTRLPE
                     FAPCRLAEVVAGVYAALRILADQRKVALRVDGLDTLPVIQADESRLFNAIYNLVNNAI
                     PEVSPGGSVTLRGRTDQAGRSILVSVIDTGRGMPAEVLKSLFTYSAISRKVGGTGLGT
                     KIVKDVVDAHGGSITVESEPGVGTSFHITLPLEGPPPHSRKASTNGPR"
BASE COUNT          640 a         1058 c         1070 g          729 t
ORIGIN      
        1 cgatgccgaa caatcctatc cagccttgga tatcgtctcg agtgtgccgg cgccgtttaa
       61 catcgccttc gtcgcctcga ccatcaagac gctcaccacc ggaaaaccgt tgttcaaagg
      121 cacggcctcc atcgagggca aactcatgga tgcggagtct ggggagatcc tcgcggccgc
      181 cgtggatcgg cgggtcggcg ggcgctttct cgacgcggag gtcttcgatt cctggaacga
      241 cgtgcacggc gccttgaagt actggtcgca gttgacccgg tttcgcttgt gtcagttgcg
      301 caagcaaacc aactgtgttt cgcccggctc gtaggtcgat cttttcgatc tcttcgcacc
      361 gatatagatc ctttgggcct tgagcgacca gcgggagttc agtgggagac cgaggaacgc
      421 ggccaggtaa cgcatggttg agagggcatg gaggtgacac aagcgaggcg agagtcttct
      481 tgacgcatcc gtccggtcgg ctgccttcaa acacctcgct cagttgaagc gttttagccg
      541 accaggtacc atgccgcctc atcttgtgtg gaggcactat ggcgcagatt accgaagttg
      601 ctccggacct gttccgcatc accaccttcc tcgaaccgtt caacctgcaa ttcagtcagt
      661 ttctggtccg tgatgccgag ccgctcctgt atcacaccgg cccgcgcgcg ctgtttccgg
      721 cggtgaagga gacggtggcg tagctcatcg acgtccgcac cttgcgctgg atcgggttca
      781 gccatttcga ggccgatgag tgcgagtcgt tgccggagtg gcagcagttg gcgccccagt
      841 ccgatgcggt ctgtagtctg gtgagcaagc tcgtgagtgt ggatgattgt ctcgcgctcc
      901 ggccggcgaa ggggatgaca gatggcgagg tgctggagac ggggaagtac cgctttcgat
      961 ttctggccac gccgcacgtg ccgcattgct gggagtccgg tctgatgttc gaagaaacac
     1021 agcggacgct gctctgctcc gatcttctgc atcaaaacgg caatgtggaa ccggtcaccc
     1081 agtcggacgt ggtgggccgc agtcgggacg tgctcgtgga atatcagcag ggtccactgg
     1141 cgaactacat gccctattgc acgctgacag atccaacatt gcagcggctg gcggcgctgc
     1201 agccgaagac cgtggcgacg atgcacggct cgctctatgt cggcgacggg ggaaaagcgc
     1261 tgcgtgattt ggccacggtc tttcgagagg tgttgggcgc tgcctgacac gttgaggcgg
     1321 gaaagcggcc gggggggcct gcttgttgac cggtggagtt tcaatcggct aggctcctcg
     1381 cgttcctgct gcatttctgc cgctcccgaa acgatccgtt gcgaggtgtt atgcgcatac
     1441 gttacgtcgt cattgcgttc agcctggccg gttgcctgac catacccggg tggggcgtga
     1501 tcagggccga accctccttt gatcagacgg ccgggtacat cctcaatatt gtcagagcct
     1561 ttcgtaccgc ttacgtgctc cacgtcgtcg aacatgcgcg agacgcggga ctgtcgccgc
     1621 gggaggattg gaaaacagat gcccattttc tcccgcttcc tgcccagttc gtcaaggaag
     1681 ccgctgaaca ggtggagggt ttggagatcg gcctcatcag tttgacgccg ctgaatccgg
     1741 cgaaccgccc ccgcacggac gcggagatga cagcgttact tcagttggag aaggatcgac
     1801 aacgcggggt catcggcttc gtcgacggcg atgaattcaa ggcggtgtcg gccgatttgg
     1861 cgctggtgcg gtcctgcgtc gattgtcaca accagcatcc ccgtgcggtg cgcaaaaact
     1921 ttcagcaatg ggatgtgatg ggcgccctgg tggtccggct caagcgcacg gtcgaagggg
     1981 aaggtcaggc cctccctccg gaacctccta agcgggcacc aggactgctc gaggggcctc
     2041 cgcctcagcc gacgattacg ccgccctggg ttcgatgacc ggctgatcgg gcccacgcat
     2101 ctggaagaga gcgggcctgg tgccggagcc agtctcggtt agcgatgatc tgcggacatc
     2161 gatcccatgc ccggtcatcg cggaccgttc gtcgaagcct tccgcgagtg gggcggtggt
     2221 ccctccaggg gaagcgtgat gtgaaacgag gtgccgacgc ctggctcgct ctcaacggtg
     2281 atgctgcctc catgggcatc gacgacatct ttcacaatct ttgtgcccag ccctgtcccg
     2341 ccgacctttc ggctgatggc gctgtacgtg aagaggctct tgaggacttc ggctggcatg
     2401 cccctaccgg tatcgatgac cgacaccagg atgcttcttc ccgcctgatc cgtacggcca
     2461 cgcagcgtga cggatccgcc gggtgacact tccggaatcg cgttgttgac gagattatag
     2521 atcgcattga acagccggct ctcgtcagcc tgaatcacgg ggagggtatc gagtccatcc
     2581 acgcgcagcg cgaccttacg ttgatcggcc aggatcctga gcgctgcgta cacgccggcg
     2641 accacctccg cgagtcggca gggcgcaaat tccggcagcc gggtgaggcc tttgaccgat
     2701 tccgccatct ccctgacccg gtcctgaatg cggcgggacc cattccggat catatccagg
     2761 agttccttgg tcagatcccg actgtgcttc agattgccgg tgacactctc cggtaggcgg
     2821 tgataacact cgtcgagttc ttcctccagc agcgccgccc cgctcatgat cggcatcagc
     2881 atattcttga tatcgtggcc gatgtctccc agcagtctcg ccacgtcggc cagggcctcc
     2941 gcttggcgca gcgcttccgc tgtctgctcg cggtccacaa actggcccac tttgatgccg
     3001 atgtcggcca ccatgtcgag aatatcctga tccggcttgc ggatctgatg gctgaaaaat
     3061 tcaataactc cgtcaatgcg ttcgccctta cggactggaa aggcaaatcc tccatgcagt
     3121 ccgacgtgag ccgcgatcga ggcccggcgg aaatgggggt cactggtcac atcaggaatc
     3181 caaataggcg ttccgctttc ccaggttcga ccgatgagcc ccacaccccg ttgaaaggtc
     3241 cgttcccggc tgtcctgtat gaactcggag gcctcaacct gcggcgcatg ccacaagtca
     3301 acgaaacgca gcaactcagc ctgcttgtct acggcccaaa acatgcccaa ttcccagtcc
     3361 agactttcac cgatagcccg aaggatttca tgacctgcgt ctttcagggt tgaggactcg
     3421 gccagcacac gggtgactgc gtattgggca cgaagccgac gttctgaccg ccgcgcttga
     3481 gccaagcagg cctctag
//
LOCUS       DAORRQ010000165         3452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_731_length_3452_cov_9.162202, whole genome shotgun sequence.
ACCESSION   DAORRQ010000165 DAORRQ010000000
VERSION     DAORRQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_3452_cov_9.162202"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="PK224_21140"
     CDS             complement(<1..2270)
                     /locus_tag="PK224_21140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRB18310.1"
                     /translation="MLRNACQYQTLVELLRVRVSDAPSKHAYTYLRDGETDALTWDYG
                     ELDRRARAIAAWLQSCGAAGERALLLYPPGLDYLAAFFGCLYAGVVAVPAYPPQRQRG
                     WPRVRAMLADSQAAYALTTSGIARVIDRLSVKEDGYGSLATLHWLNTDNVPSGVDEDW
                     SEPDITAETLAFLQYTSGSTGLPKGVMVSHGNLLHNQRLIQEAFGHTSQDVIVGWLPL
                     YHDMGLIGNVLQPLYLGASCILMSPVHFLQKPVRWLSAITRYRATTSGGPNFSYDLCV
                     RQITEAQRELLDLSSWTLAFNGAEPVRAATIERFSDRFRARGFRKEAFYPCYGLAEAT
                     LLVSGGTRGAGPLVQPFERSRLRSEPTASASGAASHLLVSCGVDRSGRQIRIVHPDSG
                     IACADGQEGEIWVGGPCVAQGYWQREAESRETFQASLAGTGERPFLRTGDLGFLKDGE
                     LFVTGRLKDLIIIRGRNHYPHDIERTIEECHPALRPGAGAAFAISEGDEEQLVVVQEV
                     EPRSQALDLQAVTTGIRQTVAQAHEVSVFAVVLIKAGTLPKTSSGKVQRSLCRDKFLA
                     RELIAVEESVLRDSSAAVVLEEEPATVGDEDGLAQVLADIWSQVLNRSNVESGDDFFA
                     LGGDSLRGLQVVARVREVRGLDVPLDALFDHPTLAAFSAHVATCVPADPGSPIESIRR
                     VSRDEILPLSLAQERFWFLDQLSAGSSINNIPVALRLVGALDVEALRRSLDEIIQRHE
                     ILRACFALRNGRPSHI"
     gene            2677..3042
                     /locus_tag="PK224_21145"
     CDS             2677..3042
                     /locus_tag="PK224_21145"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRB18311.1"
                     /translation="MKQRYTKREPVQSTCILSCEGMMGQGQLLNLSVPGCLLKTSMKL
                     KVGQYVDLRLSFPTSATALQVKLAAVRWAKNGQVGLEFIRMSETDQTRLRFLSGHIEK
                     RTPQQAWSERVVCVGTTAG"
BASE COUNT          728 a         1106 c          967 g          651 t
ORIGIN      
        1 atgatgtgtg agggacggcc gttgcgcaac gcgaaacatg cgcgaaggat ctcgtggcgc
       61 tgaatgatct catccagcga ccggcgtaac gcctccacgt caagggcacc gacaagccgc
      121 aaggcgaccg gaatgttgtt gatcgaactg cctgcagaca actggtccag aaaccaaaac
      181 cgttcctgcg cgagggacag aggcaggatt tcgtcgcgtg acacccgccg gattgattcg
      241 atcggggaac ctggatcggc cggcacacag gttgcgacat gagccgagaa agcggcaagc
      301 gtcgggtgat cgaacagcgc gtccaaggga acatcgagtc cgcggacctc ccgcacccgc
      361 gccaccacct gaagcccgcg caaggagtcg ccgcccaggg cgaagaagtc gtcgcccgac
      421 tcgacgttgg accggttcaa cacctgcgac cagatgtccg ccagcacctg cgcgaggccg
      481 tcttcgtccc cgactgttgc aggctcctcc tccagaacga ctgctgcgga ggaatccctc
      541 agcaccgact cttcgaccgc gatcaattcc cgggcgagaa atttgtcacg gcacagactt
      601 cgctgaacct ttccgctcga ggttttcgga agcgtcccgg ccttgatgag caccactgca
      661 aaaaccgaca cctcatgcgc ttgcgccacc gtctgtcgga tgccagtggt gaccgcttgc
      721 agatcgaggg cctgggagcg cggttccacc tcttgcacca ccacaagttg ctcttcatcc
      781 ccttcgctga tcgcgaaggc cgctccggct ccaggcctga gcgccggatg gcactcctcg
      841 atcgtccgtt cgatgtcgtg cggatagtga ttgcggcccc ggatgatgat gaggtctttc
      901 aatctgccgg tgacgaacag ttccccgtct ttcaggaacc cgagatctcc ggtcctgagg
      961 aagggcctct ctcctgtccc ggcaagtgat gcctgaaagg tctcccggga ttctgcttcc
     1021 ctctgccaat aaccctgagc gacgcaaggt ccacccaccc aaatctcccc ctcctgacca
     1081 tccgcacagg cgattccgga gtcgggatgg acgatgcgga tctgacgccc tgatcggtcg
     1141 acaccgcagc tcaccaggag atggcttgcc gcgccggatg cagacgccgt cggctcagat
     1201 cggagtcggg agcgctcgaa cggctgcacg aggggtcccg ctccacgggt tccacccgag
     1261 accagcagcg tcgcctcggc caatccataa cagggataaa aagcctcctt gcggaatcca
     1321 cgggctcgaa acctgtcgga gaaccgttcg atcgtcgcgg cgcgaacggg ttcggcaccg
     1381 ttgaaagcca gggtccacga actcaaatcc agcaattccc gctgcgcctc ggtaatctgc
     1441 cgcacacaga ggtcatacga gaaattcggc cccccgctgg tcgtcgcgcg gtaccgcgtg
     1501 atcgccgaca gccaccgtac cggcttctga agaaagtgca ccggggacat caagatgcag
     1561 gaggcgccaa ggtacagcgg ctgcaacacg tttccgatca atcccatgtc gtggtataga
     1621 ggcagccacc cgacgatgac gtcttgcgac gtatggccga aggcctcctg aatcaggcgc
     1681 tggttatgca acagattgcc atgactgacc atcacgccct tcggaaggcc ggttgagccg
     1741 gaggtgtatt gcaggaaagc cagcgtctcc gctgtaatgt ccggctcact ccaatcttcg
     1801 tcgacacccg acggcacgtt gtcggtattg agccagtgga gcgtcgccag actcccgtac
     1861 ccgtcctcct tgacggagag ccggtcgatc acccgcgcga tgccgctcgt cgttaatgcg
     1921 taggccgctt gcgaatctgc aagcatcgct cgaactctcg gccagccgcg ttgccgctgc
     1981 ggcggatagg ccggcacggc caccactccg gcatagagac aaccgaaaaa cgcggccaga
     2041 tagtcgagcc ccggcgggta cagcaacagc gcccgctctc ctgccgcgcc acacgactgc
     2101 aaccacgcgg cgatggccct ggcgcggcga tccaactcgc catagtccca cgtcagcgca
     2161 tccgtctcac cgtcgcgaag atacgtatac gcatgtttag atggggcgtc cgacacccgc
     2221 acacgaagga gctcgacgag tgtctggtat tgacaagcgt tgcgcagcat ctcgaatcca
     2281 ccctgccttc ccgaccgttg cgtgaatgtg tgtctactct acaagacgcc cctcatcgaa
     2341 aatcttgcat gttcactttc cggatcctgc tgctcgatca ccagatccgc cccatcgatg
     2401 gaaacaattc gtgctggacg tcgaagatga agggcctatg gacgatgacg gaaagatggg
     2461 gagggtccgc gaataatcca gtgcaggagg ttggaaaatc ggtctgcagc cgccggttga
     2521 atcatcacgc ggctgcgagt gcagtcgtca tgagttgctc acaatccacg gcagcccccc
     2581 attcgaggga cacctctcgc ggaatgacat cgttcgatcc cggcatatac ttggaccata
     2641 acgagacatg aggggctcac ctaaccagga ggcattgtga aacagcgata caccaagcgc
     2701 gaaccggtcc agagcacttg tatcctctcc tgtgaaggaa tgatggggca gggacagctg
     2761 ctgaatctct ccgtgccggg ttgcctgctg aaaaccagta tgaaactcaa ggtggggcag
     2821 tatgtggacc tgcgcctatc cttcccgacc agtgcgacgg ccctgcaagt caaattggcg
     2881 gctgttcgat gggcgaagaa cgggcaggtc gggctcgaat ttatccggat gtccgagacc
     2941 gaccagaccc ggctccgttt cctctccggc catatcgaga aacggacgcc tcaacaggct
     3001 tggagtgagc gggttgtctg cgtgggcacc accgcgggat agccagttgg agcccgatca
     3061 gcatccgcca tgggaatggt ctccactcta accgtcccgc atcaagaggc tcctctccac
     3121 gatgggaatg cgccccggag gtgcattccc atctcccccc atatctccac ctccgaacaa
     3181 ctacggactt gcacgaccac ctgacggcat ggttgactga aaaagggtga aggtgtctgc
     3241 tttggttgtg cccacccagg gcggcaagag gtctcgacat cctcctggcg ggatctgcct
     3301 tcacccccga atttatcttc tattctcttt gccctcccgg cgctcccagg accatccctt
     3361 atcttcagct tcctgctcct ggcaatatct cccaaatcac atcatgtaca tcgcatatat
     3421 atttgatatt taaagattac tatctacatg ca
//
LOCUS       DAORRQ010000166         3400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_737_length_3400_cov_8.327354, whole genome shotgun sequence.
ACCESSION   DAORRQ010000166 DAORRQ010000000
VERSION     DAORRQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_737_length_3400_cov_8.327354"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PK224_21150"
     CDS             complement(<1..492)
                     /locus_tag="PK224_21150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRB18312.1"
                     /translation="MKREATTDKGGVTLSTGHPQLLAEIRAEIAATGPIPFARFMDLA
                     LYHPKYGYYVRPVDDPTQERIGWSGDFYTSSDVHPILGQALARQAQQLDTLLGHPDPF
                     TVVEMGAGKGLLARDFLTACRNAPANLRDRLRYILIERSAAMRTQQQNNLAPWVGQAG
                     RVAW"
     gene            complement(670..3375)
                     /gene="secA"
                     /locus_tag="PK224_21155"
     CDS             complement(670..3375)
                     /gene="secA"
                     /locus_tag="PK224_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRB18313.1"
                     /translation="MSRSSSGCIFGSKNDREIKALRPIVERINGLESSFTPLSDQALA
                     EKTQEFKKRLEDGETLEDILPEAFAVCREMSRRRLNMRHFDVQLIGGMILNKGRIAEM
                     KTGEGKTLVATLPLYLNALEGKGAHLVTVNDYLAKRDAQWMAQLYHALGLSVGIIQHD
                     ASFLYDPTYEASDKRLQHLRPCTRHEAYRAEITYGTNNEYGFDYLRDNLIVSDLSQCV
                     QRPLNFAIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINSIIPQLKAEHDYTIEEK
                     TKTASLTEEGNVRVEKLLGVDNLYDLQHMDLVHHVVKALQAYSLYKRDVDYVVKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYKKLAGMT
                     GTADTEAGEFAKIYNLDVNVVPTNRSMVRKDYADVVFRTEKEKFTAIVEEIKDCNERG
                     QPVLVGTVSIEKSERLSGYLSRSGVKHNVLNAKFHEKEAEIIAQAGRKGAVTIATNMA
                     GRGTDILLGGNADFLFKRILYQDENLTDARKQEIFDQIKADCEKDKQEVVAAGGLHIL
                     GTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFASERVSQLMLKLGMEEG
                     VPIEHGMVTRAIANAQKKVEAHNFEIRKQLLEYDDVMNKQREVIYQHRHAVLAGEHIQ
                     QDIHDMMRDIVNAFVETYCPADQYQEEWDFNGLGEALQGQFALDVTQGHGSVADHFKD
                     VGRDALIEDIQNQVRRAYDHKEQELGSELMRYLEKMLLLQVIDHHWKDHLLGMDHLRD
                     GIGLRGYGQKDPLIEYKREGFDMFSSMMDRIKSDVLERMFRVQAVRGEQPPPPAPEPT
                     PPPRMVLNRSDEPASQTVQSQADKTGRNDPCPCGSGKKFKKCHGA"
BASE COUNT          589 a          975 c         1032 g          804 t
ORIGIN      
        1 ccaggcaacg cgaccggcct gtcccaccca gggcgccaga ttgttttgct gctgggtgcg
       61 cattgcggcg ctgcgctcga tcaagatgta tcgaagacgg tcgcggaggt tcgcgggcgc
      121 gttccggcaa gcggtcagga aatcccgcgc cagcagtccc ttgcccgctc ccatctccac
      181 caccgtgaag gggtctggat gcccgaggag ggtgtcgagt tgttgtgcct gtctggctaa
      241 cgcctggccg aggatcggat gcacgtcgga actcgtataa aaatcgcccg accatccgat
      301 gcgttcctgg gtcggatcat cgacggggcg aacatagtag ccgtacttcg gatgatacag
      361 ggcgaggtcc ataaaacggg cgaacggaat cggaccagtg gctgcaattt cagccctgat
      421 ttccgccagg agttgtggat gcccagtgct caaagtgaca ccacccttgt cggtagtcgc
      481 ctcccgtttc atgcgccatc gctagaaaca gcacttctac cggattctct agggaaaaga
      541 gggggttaga ttagccgctg cctcagcggc aagtcaaggc gaaaagacct gatcaggtgg
      601 gagaatctcg atgttcgacg gagtgtttgc tcgatccgtc gtgagtgaag ggggagtgcc
      661 tgttccctgt tacgccccgt ggcacttctt gaattttttc ccgctcccgc aggggcaggg
      721 atcgttgcga ccggttttgt ccgcctggct ctggaccgtt tgcgaggctg gttcgtcgct
      781 gcgattgagc accatgcgag gcggaggggt cggctcgggt gcgggagggg gcggttgctc
      841 gccccgcacg gcctggacgc gaaacatccg ttccagcaca tccgacttga tccggtccat
      901 catggaggag aacatgtcga acccttcgcg cttgtactcg atcagcgggt ccttttgccc
      961 atatccgcgc aggccgatgc cgtcccgcaa atggtccatg cccagcaagt gatctttcca
     1021 gtggtggtcg atcacctgga gcagcagcat cttttccaga taccgcatca gctcggagcc
     1081 gagttcctgt tccttgtgat cgtaggcccg ccggacctgg ttctgaatgt cctcgatcag
     1141 cgcatcccgc ccgacgtctt tgaaatggtc tgcgacgctg ccgtgaccct gagtgacgtc
     1201 cagagcgaac tggccctgca gcgcttcgcc aaggccgttg aaatcccatt cctcctggta
     1261 ctgatcggcc gggcagtagg tctccacgaa ggcgttcacg atgtcccgca tcatgtcgtg
     1321 aatatcctgc tggatgtgct cgccggccag cacggcgtgg cggtgctggt agatcacttc
     1381 ccgctgcttg ttcatcacgt cgtcgtattc aaggagctgt ttgcggattt cgaagttgtg
     1441 cgcctcgact tttttctgag cgttggcgat ggctctggtg accatcccgt gttcgatcgg
     1501 cacgccttct tccatgccca gcttgagcat caactgggac acgcgctcgg aggcgaaaat
     1561 gcgcatcagg tcatcttcga gcgacaggta gaagcgggag gagccgggat cgccctgacg
     1621 gccggcgcgg ccacggagct ggttgtcgat acggcggctt tcgtgccgct cggtgccgag
     1681 gatatggagc cctccggcgg caaccacttc ctgcttgtcc ttttcgcaat cggccttaat
     1741 ctggtcgaaa atttcctgtt tccgtgcgtc cgtgaggttt tcgtcctggt acaggatccg
     1801 tttgaacagg aagtcggcgt tgcctcccag gagaatgtcg gtgccgcggc ccgccatgtt
     1861 ggtggcgatg gtcacggctc ccttgcgtcc cgcttgcgcg atgatctccg cttctttctc
     1921 atggaacttg gcattcaaga cattgtgctt cacgccgctt cggctcaggt agccggacag
     1981 ccgctcggat ttttcaatgg agacggtgcc gaccaggacc ggctgcccac gttcgttgca
     2041 gtccttaatt tcctcgacga tcgccgtgaa cttttctttt tccgtccgga agacgacgtc
     2101 ggcgtaatcc ttgcggacca tcgaccggtt ggtcggcaca acgttgacgt ccagattgta
     2161 gatcttggcg aactctcccg cttccgtgtc ggccgtaccg gtcatgccgg ccagtttctt
     2221 gtacatacgg aagtagttct ggaaggtgac ggaggccaga gtctggttct cgttggcgat
     2281 cttgacgcct tctttggctt ccacggcctg gtgcaacccg tcgctccatc ggcggcccgg
     2341 catcaatcgg ccggtaaact catcgacgat gatgacttcg ccgtccttga ccacataatc
     2401 gacatcgcgc ttgtagagtg aataggcctg gagcgccttg acgacgtggt ggaccaaatc
     2461 catgtgctgc aggtcgtaca ggttgtccac gcccaatagt ttctctacac ggacgttgcc
     2521 ctcttccgtg agcgaggcgg tcttggtctt ttcttcgatc gtatagtcgt gctccgcctt
     2581 gagctgcggg atgatcgaat tgatgcggta gtacagatcg gtcgtttgat cggtgggacc
     2641 ggagatgatc aatggcgtgc gcgcttcgtc gatcagaatg ctgtcgactt cgtccacgat
     2701 ggcgaagttc agcgggcgtt ggacgcattg gctcaggtcg ctgacgatca ggttgtcgcg
     2761 caggtaatcg aacccgtatt cgttgttggt gccgtaggtg atctcggcgc ggtaggcctc
     2821 atgtctggtg cagggccgca aatgttgcag gcgcttgtcc gacgcttcat aggtcggatc
     2881 atataaaaag gacgcatcgt gctggatgat gccgacggag aggccgagtg cgtgatagag
     2941 ctgcgccatc cattgggcgt cgcgtttggc caggtaatcg ttgacggtga ccaggtgggc
     3001 gcctttgcct tcgagcgcat tcaaatagag gggcaaggtg gcgaccaggg tcttgccctc
     3061 accggtcttc atttcggcga tccggccttt attcaggatc atgccgccga tgagctgcac
     3121 gtcgaaatgc cgcatattca agcgacgacg ggacatttcc cggcacacgg cgaacgcttc
     3181 gggcagaatg tcttccagcg tttctccgtc ttcgagccgt ttcttgaact cttgcgtctt
     3241 ctcggccagt gcctggtcgg acaagggcgt gaagctggat tcgagtccat tgatgcgctc
     3301 gacgatcggg cgcaacgcct tgatctcgcg gtcgtttttg cttccgaaga tgcaaccgct
     3361 gcttgaacga ctcatgacta cctttcccga cgccgtccgc
//
LOCUS       DAORRQ010000167         3266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_761_length_3266_cov_7.597633, whole genome shotgun sequence.
ACCESSION   DAORRQ010000167 DAORRQ010000000
VERSION     DAORRQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_761_length_3266_cov_7.597633"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1263
                     /locus_tag="PK224_21160"
     CDS             184..1263
                     /locus_tag="PK224_21160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB18314.1"
                     /translation="MRQGANFSLSLITSVPLLYLVVFVSPVDVSAASSASSDSARRSY
                     ERGVTLFREGNADGAIEALKKALAQNPKLAEAYHVLGLVYFQNKRNPDEAIQAYKQSL
                     KLGPASAEILNDLADVYLAQGRGSDAEGVLRQALDIAPGNEEAHLDLARLYDARHDRA
                     NAVKMYQSLLRVRPDHADALYHLASLYDSQGDLKLAREYLSRLTQANPGHADAWYLLG
                     RLAERGNDLNAAAAAFKEAIAVKADLVDAHYNLGFVYRSQGLLAEAEREFLEVIRYRP
                     EYAEAHLNLGMVYTSLNRLEEAEKEHERAVALKPQSAEAHYNLGVFYELHRKDMGRAL
                     AQYRRYRDLGGRDERVERIIGMGGP"
     gene            1359..1664
                     /locus_tag="PK224_21165"
     CDS             1359..1664
                     /locus_tag="PK224_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18315.1"
                     /translation="MRSPHFRSIVWVVAFAVIALLAVTTGSAVAHELQHAAHHDAGMH
                     GSGICAWMCATAGVHVVTPVHSTHLFTVAGDASFRSDTVLLPQRLAPSRPRAPPLFA"
     gene            1797..3071
                     /locus_tag="PK224_21170"
     CDS             1797..3071
                     /locus_tag="PK224_21170"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRB18316.1"
                     /translation="MVPVREIGVAVCGAATLLALVGFSSGASAHNESQAVTMLGAPLT
                     LVQSTTALSPPTLRFHRDGRLHAAWIEKNGVQGEVKTAAISADHSAVAQVSVNPPGAG
                     PEAVHQSPGFAIGVDGEQFVTWSSANKTPGALFASDLRLARLDSGGARVQPPVQVNDD
                     GLAISHTFEHVIAGKNGEIYVAWLDGRNKDRSGAGIMFACSQDHGATVGKNLVLDGMA
                     CPCCRPMLAQAPDGSLWVAWRKTFEGNVRDIVVARSTDQGRTFSPPTVVSRDGWVFDA
                     CPHRGPSIAFDGRGRLYIGWYTEGKDEQPRLFISTSDDQGATFSAPVALHNATTSLPD
                     NLHMAVHPGGRVVAVWEEVTGVRKRVVMRVSDDRGQTFSPMQTLSSGSKAEHPTVAIH
                     DSGTVALGWTEHAFPHNRIIVQQGQLSRVVTP"
BASE COUNT          596 a          948 c         1035 g          687 t
ORIGIN      
        1 gagtatacag gaatccatag gggaatccta cccaaacagc gccggaggtc tcaactgctt
       61 tgcaatgact ggagccgacg ggctcctgtc gttgctttcg catgcgctgc gatttatagt
      121 ggggctcacc gcttcgccgg ttctacaggc gaaggtcacc cacggattcc actgcggatt
      181 tcgatgcggc aaggggccaa cttctctctc agtctcatca cgagcgttcc gctcctctac
      241 ctggttgttt tcgtatcacc cgtcgacgtg tcggccgcct catccgcgtc gtccgactcg
      301 gcgcggcgca gttatgagcg gggcgtcaca ttgtttcgcg aaggcaatgc cgacggcgcg
      361 attgaggcgt tgaagaaggc gttggcacag aatcccaagc tggccgaggc ctaccatgtg
      421 ttgggacttg tttattttca gaataagcgc aatcccgacg aggcgatcca ggcctacaag
      481 cagtcgctga aattaggacc ggcttccgcg gagattctga acgatctcgc cgatgtgtat
      541 ctggcgcaag gacgtggcag cgacgcggag ggggtgttac ggcaggcgct ggatatcgcg
      601 cctggaaatg aagaagccca tttggatctg gcgcgcctct atgacgcacg gcatgatcgc
      661 gcgaatgccg tgaagatgta tcagtccctc ctgcgggtgc gtcctgacca tgccgatgcc
      721 ctgtatcatt tggcgagtct ctacgacagt cagggggacc tcaagctggc ccgcgagtat
      781 ttgtcccgtc ttacgcaggc gaaccccggg catgcggatg cctggtatct cctcgggcga
      841 ctggccgagc gcggcaacga cctcaacgcc gccgccgctg cattcaaaga agccattgcg
      901 gtcaaggccg atctggtcga tgcccattac aacctggggt ttgtctatcg gagtcagggg
      961 ctgcttgccg aggcggagcg ggaatttctg gaggtgatcc ggtatcgccc agaatatgcg
     1021 gaagcgcatt tgaatctggg tatggtctac accagcttga accggctgga ggaggcggag
     1081 aaggagcacg agcgggcggt ggccctcaag cctcaatctg cggaggccca ctataacctc
     1141 ggggtctttt acgagctcca tcgcaaggac atggggcggg cgctggcgca ataccggcgc
     1201 tatcgggacc tcggcggccg ggacgagagg gtcgagcgca tcatcggcat gggcggaccg
     1261 taaccggtct cggtcgaagg ccctcgggag gccaaaagtg acgttgacgc ttcctccatc
     1321 agtttgctag catgccgacc atatctcggt ctttttctat gcgtagtcct cattttagaa
     1381 gcatcgtctg ggtggtcgca ttcgccgtca ttgccttatt ggcagtgacg accgggagtg
     1441 cggtcgcgca tgaactccaa catgcggcac atcacgatgc cggcatgcat ggatccggca
     1501 tttgtgcctg gatgtgtgcg acggccgggg tgcacgtggt gacccccgtt cactcgacgc
     1561 atctgttcac cgtggccggt gacgcttcgt tccgctctga tactgtgctc ttgccccaac
     1621 gactcgcccc gagtcggcct cgcgctccac ccctgttcgc ctaatccaag tctccgttgt
     1681 gcctgtctcg ctccgtgact cgatgtcgca gagcccgagg aaacagttcg gcgaggtgtc
     1741 tcgcatgctg tgggtgcatt ccttctttca gatcgtcgga atacgaggac actgtgatgg
     1801 tccctgtgcg tgagatcggc gttgccgtgt gtggtgctgc gaccctgctg gccctcgtcg
     1861 gcttcagcag cggagcctct gcccacaatg aatcccaggc tgtcacgatg cttggggcgc
     1921 cgctcacact cgtccagtcc accacggccc tctctccccc cacacttcgg tttcatcgcg
     1981 atggaaggct gcacgcggcc tggattgaaa aaaacggggt gcagggggag gtgaagaccg
     2041 cagctatttc cgctgatcac tcggcggtcg cccaggtttc cgtcaatccg ccgggtgccg
     2101 ggccggaagc cgtgcatcaa tcaccgggtt tcgccatagg cgtggacgga gaacagtttg
     2161 tcacctggtc ctctgccaat aagacacccg gagccttatt cgcgtcggat ctccgcctcg
     2221 cgcgattgga ctcggggggc gcgcgtgttc agccgccggt gcaggtcaat gacgatggct
     2281 tggccatttc gcataccttt gagcatgtga ttgccggaaa aaatggcgag atctatgtcg
     2341 catggctgga tggacggaat aaggaccgga gcggggcggg tatcatgttc gcttgctccc
     2401 aggatcatgg cgccacggtc ggtaagaatc tcgtcctcga cgggatggcc tgtccctgtt
     2461 gtcgtccgat gctcgcgcag gcgccggatg ggtcgttgtg ggtcgcctgg cgcaagacct
     2521 tcgagggcaa tgtgcgggac attgtggttg cgcgttcgac cgaccagggt cgaacctttt
     2581 ccccgccgac cgtcgtgagt cgggacggat gggtctttga cgcctgtccc catcggggcc
     2641 cctcgatcgc attcgatggc cggggacgcc tctacatcgg gtggtacacc gagggcaagg
     2701 acgaacagcc gcgcctcttt atttcaacat cggacgatca gggcgcgacg ttttccgcgc
     2761 cggtagcgct acacaatgcc acaacctctt tgccggacaa tctccacatg gccgtccatc
     2821 cgggtggccg ggtcgtggcg gtgtgggagg aagtgacggg ggtgcggaag cgtgtggtga
     2881 tgcgggtttc tgacgatcgc gggcagacat tcagcccgat gcagaccttg agcagcggct
     2941 ccaaggccga gcatccgacc gtggccatcc acgacagtgg aacggtggcg ttgggctgga
     3001 ccgaacatgc ctttccgcac aacagaatta tcgtacaaca ggggcaactg tcgcgcgtgg
     3061 tgacacccta gcgcggtccg gcgattccta tttgtagcag gttgcggaaa cactcggggt
     3121 tcgttcaata gccgtcgtta tctgaagtgc gacgacaacg acgggataat tcgcaggatg
     3181 ctcacaaagg ccgttcagca aggccgcagc aagcgaaggg gcgaagcgta ctgctcccgt
     3241 acggtgagtc tctgaacgtt gcgaga
//
LOCUS       DAORRQ010000168         3228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_769_length_3228_cov_59.276395, whole genome shotgun sequence.
ACCESSION   DAORRQ010000168 DAORRQ010000000
VERSION     DAORRQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_769_length_3228_cov_59.276395"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..581)
                     /locus_tag="PK224_21175"
     CDS             complement(150..581)
                     /locus_tag="PK224_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18317.1"
                     /translation="MLRTLSLLILLGLLIVEAGCSTPSGPRLTGAVRYFNIRSEVAPR
                     HLIVQVGDEIRWQNLNSQAVRLRMLEETNPELISCHKGFSQFGVLQDSVTIAPMQSVS
                     LCFSKPTTVRFNVWLEADNPQGAMTPTGSIRIEPSASKRSS"
     gene            723..2045
                     /locus_tag="PK224_21180"
     CDS             723..2045
                     /locus_tag="PK224_21180"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRB18318.1"
                     /translation="MSLTFDEARHFLSRTAFGGTPEEIRRVMQLDRAAAVAQALAIPT
                     NKAQTTPPAWIHRLPPLPKVRKHWSEAQRKTFREGLKADGQELKAWWYRELLTSRCPL
                     LERMTLWWHNHFTSSLHKVKWPPFLYQQNVLLRLYALGSFRTLLTAIARNPAMLLYLD
                     TQSNRKEHPNENFARELFELFTLGEGHYTEQDIKEAARAFTGWHLDFHTGAFRVDLRH
                     HDDGRKVVFGKSGPFEGDDILLLTLEQPQVARYIVGKLWREFISDRPDSLEVDRLADS
                     FRKKNYEIAGLLQDLLLSPQFWAPENRGVLIKSPVELIVGTARLFSLPIEDTVQQIGL
                     ARRLGQDLFDPPNVKGWPGGTRWITTSSLLDRTQMLHRAIRGHETGHSHGMADMGHVP
                     GRLWLATEPIEAVQATLLPLPPVQPLMEGEERGQAVHQLVLDPVYQLK"
     gene            2053..>3228
                     /locus_tag="PK224_21185"
     CDS             2053..>3228
                     /locus_tag="PK224_21185"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRB18319.1"
                     /translation="MNGKGLDRRTLLKAAVALPLVLLRPQWLPELRAAEKNGAGAGAG
                     NRERLLLLVELHGGNDGLNTLIPYAEAAYYQARPQLAISRDQVRQLTPQFGLHPALTP
                     LMPFWENKELACLHGVGYPRPNRSHFRSIEIWETASDSEQVLDTGWLSRVFEQHPLPA
                     RFTAEGIVLGKGDSGPLSGGKTRTIALHDPGQFLQQAGSVRPASLSTSNPALAHILEV
                     QREISQAASDLQGRIRQAPPLTSDFPANKIGKQLEVAAKLIAAQVPVAVIKVTQGSFD
                     THAGQSATHHRLLEELAQGLTTFRAAMEKLGAWKDVLVMTYSEFGRRVGENASHGTDH
                     GTAAPHFFMGGRVKGGLYGVSPSLRDLQDGDLKHTLDYRAVYATVIEKWWGLPAVSFG
                     "
BASE COUNT          635 a          919 c         1023 g          651 t
ORIGIN      
        1 gatggggtcg caagatccgt cctcacgcta catgcggcct accgtgattc ctccgctctt
       61 ctgcgatccg tccatcaata ttccctgctg acgaaccgtt gcggatagcc agtcttcggc
      121 cggcgcacgt ggctgcgtga tggctggttt taggagctgc gtttcgaggc ggatggttcg
      181 atccggatgc ttccggtggg ggtcatggcg ccttgtggat tgtcggcctc gagccagaca
      241 ttgaagcgca cggtggtggg tttcgagaag cagaggctga cggattgcat gggagcgatg
      301 gtcaccgagt cctgtaggac tccaaattgg gaaaaccctt tgtgacagga gatcaactcg
      361 gggttggttt cttcgagcat ccgtaaccgg actgcctggg aattcaggtt ctgccatcgg
      421 atttcatcgc cgacttgcac gatcaaatga cgaggcgcga cctccgagcg gatattgaaa
      481 tagcggaccg caccggtgag gcgcgggccg gatggggtgc tgcaaccggc ctctacgatg
      541 agcaggccga gtaaaatcag aagcgacagc gttcgaagca tgcgcgatac tacagaacta
      601 tgccgcgcgt tggctactag gattgttacc aggtagggcg acgagaagaa accctgtcaa
      661 cccggttgcg acagagtcgt tgtcagagag agcgccgcgt tcattgttcc gagagttcat
      721 gcatgagtct gaccttcgac gaggcccgcc actttctctc ccgcacggcg ttcggtggaa
      781 cgcctgaaga aatccggcgt gtgatgcagc tggatcgcgc cgccgcggtg gcgcaggcgt
      841 tggcgattcc cacgaacaag gctcagacca cgccgcctgc ctggatccat cgtctccctc
      901 cgttgccgaa ggtgcgtaag cactggagcg aggcgcagag gaagacgttc cgcgaaggac
      961 tgaaggcgga tgggcaggaa ctcaaggctt ggtggtatcg ggaactcctc acttcgcggt
     1021 gcccgctgct ggagcgcatg acgttatggt ggcacaacca ctttacgtcc agtttgcaca
     1081 aggtgaagtg gccgccgttt ttgtatcagc agaatgtcct gctgcggctc tatgctctgg
     1141 ggtcgtttcg cacgctgctc accgccatcg ccaggaatcc ggcgatgctt ctgtatctcg
     1201 acacacagag caaccgcaaa gaacatccca atgaaaattt tgcccgtgag ttgttcgagc
     1261 tcttcacgtt gggagaaggc cactataccg aacaggacat caaggaggct gcacgggcat
     1321 ttaccgggtg gcatctcgat ttccataccg gcgcgttccg tgtcgatctg cgtcatcatg
     1381 acgacggacg caaagtcgta ttcggaaagt ccggtccctt cgagggcgat gatattctct
     1441 tgctgacgct ggagcagcct caggtggccc ggtacatcgt cgggaagctc tggcgtgaat
     1501 ttatttcaga ccggcccgac tcgctggaag tggatcgtct ggccgactca ttcaggaaga
     1561 agaactatga gatcgccgga ttgctccagg acctgctcct gagcccgcag ttctgggcac
     1621 ccgagaaccg tggtgtactg atcaagtctc cggtcgagct cattgtgggc accgcaagac
     1681 tcttcagtct accgatcgaa gacaccgtgc aacagatcgg gttggcccgc cggctcgggc
     1741 aggatctctt cgaccctccc aacgtgaagg gttggccagg tggtacacgg tggatcacaa
     1801 cttcgagcct gctggaccgc acgcagatgc tgcatcgtgc gattcgcggc cacgagaccg
     1861 gtcattcgca cgggatggcc gatatgggcc acgtgcccgg ccggctgtgg ctcgcgacgg
     1921 agcccatcga ggccgtgcag gctacgctac tcccgctccc cccggtgcag cccttgatgg
     1981 aaggggaaga gcgcggccag gccgtgcacc aactggtatt ggatccggtg tatcaactca
     2041 agtgaggccg ccatgaacgg gaaggggttg gatcgaagaa cgttgctgaa agccgcagtg
     2101 gctctgccgc tcgttctgtt gcgtccacag tggttgccgg aactccgggc agcggagaag
     2161 aacggggctg gtgccggagc aggcaaccgg gaacggctgt tgctgttggt tgagttgcat
     2221 ggcgggaacg acgggctcaa cacgctcatt ccctacgcag aggcggccta ttatcaggcc
     2281 cgaccgcaat tagcgatttc gcgtgaccag gtccgccaac tcacccctca gttcggactc
     2341 catccggcgc tgaccccgct gatgcccttc tgggagaaca aggagctggc ttgcctgcat
     2401 ggggtcgggt atccccgacc gaatcgttcc catttccgat ccatcgagat ttgggagacg
     2461 gcgtcagaca gtgagcaggt gctggacacc ggctggctct cgcgtgtgtt tgagcaacat
     2521 ccgctgccgg cgcgattcac cgctgagggg attgtgcttg gcaagggcga tagcggtcca
     2581 ttgagtggag ggaagacccg caccattgcc ctgcatgatc cggggcagtt tctacagcaa
     2641 gccgggtccg tcagaccggc ttccctctcg acgagcaacc ctgctctggc ccatattctg
     2701 gaggtccagc gggaaatctc ccaggccgct tccgatctac agggccgtat tcggcaggcg
     2761 ccgccactca cgagcgactt tccggcgaat aagatcggca aacagctgga ggtcgcagcc
     2821 aagctcatcg ccgcgcaggt gccggtcgca gtcatcaaag tgacgcaagg aagtttcgat
     2881 acccatgccg gccagtcggc gacacatcac cggttgttag aggaactggc gcagggcctg
     2941 acgaccttcc gcgcggcaat ggagaagctg ggcgcctgga aggatgtgct ggtcatgacc
     3001 tattcggagt tcgggcgccg ggtaggagag aatgccagcc atggaaccga ccacggcacg
     3061 gcggccccgc actttttcat gggcgggcgc gtgaagggcg gcctctatgg agtatctccc
     3121 tcactgagag atctccagga cggggatctg aagcatacgc tcgactatcg ggctgtgtat
     3181 gcgaccgtca ttgagaaatg gtggggattg cctgccgttt ctttcgga
//
LOCUS       DAORRQ010000169         3211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_774_length_3211_cov_5.540558, whole genome shotgun sequence.
ACCESSION   DAORRQ010000169 DAORRQ010000000
VERSION     DAORRQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_3211_cov_5.540558"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..>3211
                     /locus_tag="PK224_21190"
     CDS             80..>3211
                     /locus_tag="PK224_21190"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRB18320.1"
                     /translation="MGHRRDMDLAVDVPKDELGAVATNAIWSDIYDRVAALVEAHRST
                     LVFVNTRRLAERVSHYLEERLRHLGDEVVAAHHGSLSRTIRLSAEDRLKTGAVRVVVA
                     TASLELGIDVGTVDLVCQIGSPRSIATGLQRIGRAGHWIHAIPKGRLFATTRDELIEC
                     AALIRAIRAGVLDRIEVPPAPLDVLAQQIVAAAATQSWDEAELYDLCRRAMPYRDLDR
                     STFDAVVTMLADGYVTSRGRGRAYLHHDRINHHIRGRRGARLAAITSGGAIPDTANYA
                     VIAEPDGTVVGSVDEDFAVESLAGDIILLGNTSWRIKGVETGKMRVEDAQGAPPTIPF
                     WRGEAPARTAELSAEVARLKADIDHRLTTDQALSMASAPPVQWLRQECGLDQRGAQQA
                     VEYILAGKAVLGTVPTRQTIVAERFFDESGGMQLVIHAPFGGRINRAWGLALRKRFCV
                     TFDFELQAAATDEGIVLSLGEKHSFPLETVFAFLNVNTLREVLTQAVLQAPMFMTRWR
                     WNASRALALLRFVGGKRVPPQIQRMRAEDLLAAVFPDAIACQDNFQGARTIRQIPDHP
                     LAQETIRDCLTEAMDLDGLIAVLEKIERGAIACLAVDTPMPSAFCHEILNANPYAFLD
                     DAPLEERRARAVDMRRSLPPELAGGMGALDQSAIDQVSEESWPVVRDAEEFHDALLSL
                     GWVPCARMPGWDVLVPKLAAAGRVATLWQGETKLGWLAAEYRHYAGLLFPDARIDPVT
                     GPVDPTEQVEQEEVLNRVVLGWMESIGPTTAGELSQTLHLSESDVQSTFLRLESQGHL
                     LRGQFRQALSARSETKDPSFHLSPFTSHEFCHRRLLARIHRLTIGRLRKEIEPVTAAE
                     FMRFLFQWQHAAPGARLHGEAGLLEVVKQLGGFEAAASAWESQILRVRMAKYQPEWLD
                     RLCLSGALMWGRLTPHPRLMQELNPVQGRRVIPTRVAPVGIFAREDGPVLLAAAGEEL
                     ARLDLSARLSGSAQAIRGCLQARGASFFSELLHGTRLLASEVEDGLWELVAAGLVTAD
                     GFDNLRSLIDPKRR"
BASE COUNT          513 a          929 c         1134 g          635 t
ORIGIN      
        1 cgtcaacggt cacacgtcat gcgaagccgt cagtcataag ccattagcca tcagctctgc
       61 gccgtgcacg atcatcgacg tgggccatcg ccgcgatatg gatctggccg ttgacgtgcc
      121 gaaggatgaa ctcggggccg tcgcgacgaa tgcgatctgg agcgatatct atgatcgtgt
      181 cgccgcgctg gtcgaggcgc atcgctccac cctggtcttt gtgaatacgc ggcgactggc
      241 tgagcgggtc tcgcattatt tggaagagcg gttgcggcat cttggggacg aggtggtggc
      301 ggcgcatcac ggcagtctgt cgcgaacgat ccgcctgtcg gccgaagatc gattgaaaac
      361 gggcgcggtg cgggtggtgg tggccacggc ctcgctggaa ctcggcatcg atgtcggaac
      421 cgtcgatctc gtgtgccaga tcgggtcccc ccgctcgatc gccaccggac tccagcggat
      481 cggtagggcg ggccattgga ttcatgccat tcccaagggc cgtctctttg ccaccacgcg
      541 tgacgaattg atcgaatgtg ccgccttgat ccgggcgatc agggccggag tgctggaccg
      601 catcgaggtg ccgcctgcgc cgctggatgt gttagcgcag cagatcgtgg ccgccgcggc
      661 gacacagtcg tgggatgagg cggagctcta cgatctctgc cggcgcgcga tgccctaccg
      721 cgatctggat cggtcgacct tcgacgccgt cgtcaccatg ctcgccgatg ggtacgtgac
      781 cagccggggc cgggggcggg cctacctcca tcacgaccgc atcaatcacc acattcgggg
      841 ccgtcgcggc gctcgcctgg cggcgatcac ctcaggcggt gcgatccccg acacggccaa
      901 ctacgccgtg attgcggagc cggacggcac ggtggtgggg tccgtcgacg aggatttcgc
      961 cgtcgagagt ctggccggag acatcatcct gctgggcaat acgtcctggc ggatcaaagg
     1021 cgtggagacc ggcaagatgc gggtcgagga tgcgcagggc gctccgccga cgattccgtt
     1081 ctggcgcggg gaggcgccgg cgcgtacggc ggaactgtcg gcggaagtgg cgcgtctcaa
     1141 ggccgacatc gatcatcgac tgactaccga ccaggcgctc tccatggctt ctgcaccgcc
     1201 cgtgcagtgg ctcaggcagg aatgtggtct cgaccagcgg ggggcgcaac aggcggtcga
     1261 atatatcctg gctggcaagg cggtgctggg gacggtcccg acgcggcaga ccatcgtggc
     1321 ggagcggttt ttcgatgaaa gcggcgggat gcagctggtc atccatgcgc cgttcggcgg
     1381 tcggatcaat cgggcctggg gattggcctt gcggaagcgg ttttgtgtga ccttcgactt
     1441 tgagttgcag gccgccgcca cggatgaagg cattgtcctg tcgttggggg agaagcacag
     1501 ctttccgctc gagacggtgt ttgcgttcct gaacgtcaac acgttgcggg aggtgctcac
     1561 ccaggcggtg ctgcaagcgc cgatgttcat gacacgttgg cggtggaacg cttcgcgggc
     1621 cctggccttg ctccggtttg tcggagggaa gcgagtgccc ccgcagattc agcgaatgcg
     1681 cgccgaagat ctccttgcgg cggtgtttcc cgatgcgatt gcctgtcagg ataatttcca
     1741 gggtgcgcga acgatccggc agattccgga ccatccgttg gcgcaggaga cgatccgcga
     1801 ttgtctgacg gaagcgatgg atctcgacgg cttgatcgcc gtgctggaga agatcgagcg
     1861 cggcgcgatc gcctgtctgg cggtcgacac cccgatgccc tcggccttct gccacgaaat
     1921 tttgaacgcc aatccctatg ccttcctcga tgatgcgccg ttggaggaac gccgggcccg
     1981 cgcggtggac atgcggcgca gtttgccgcc tgaactggcg ggtggcatgg gcgcgctgga
     2041 tcaatcggcc atcgatcagg tcagtgagga gtcctggccg gtggtgcggg acgcggagga
     2101 attccacgat gcgctgttgt cgctgggctg ggtgccctgt gcgcgcatgc ccggctggga
     2161 tgtgctggtg ccgaagctgg cggcggcagg gcgtgtcgcc acgctctggc agggtgagac
     2221 gaagctgggg tggctggcag cggagtatcg ccattacgcc gggttgctgt ttcccgatgc
     2281 gcggatcgat ccggtgaccg gcccggttga tccgacggaa caggttgagc aggaagaggt
     2341 cttgaaccgg gtggtgctgg gatggatgga gagtatcggg ccaacgacgg ccggggaact
     2401 gtcgcagacg ctgcatctgt ccgagtcgga cgttcagtcc acgtttctgc gactcgagtc
     2461 ccagggccat ctgctccgtg gccaattccg tcaggcactg agtgcgagat ctgaaacgaa
     2521 agatccatcg tttcaccttt caccgttcac ttctcacgag ttttgtcatc gccgcctgct
     2581 cgcccggatt catcgcttga cgatcgggcg gttgcgcaaa gaaatcgagc ctgtcacggc
     2641 cgccgagttc atgcgtttcc tgtttcagtg gcaacatgcg gcgcccggcg cgcgcctgca
     2701 cggagaagcc gggcttcttg aagtcgtcaa acaactcggt ggattcgagg cagccgcctc
     2761 ggcctgggaa tcacagatcc tgcgcgttcg catggccaag tatcagcccg agtggctcga
     2821 tcggctctgt ttgagtgggg cgctgatgtg gggtcgtctg acgccgcatc cccgtctgat
     2881 gcaggaactg aatcctgtcc agggacgtcg cgtgattccg acccgggttg cgcccgtcgg
     2941 tatctttgcg cgcgaggatg ggccggtctt gctggcggcg gctggtgagg agttggcgcg
     3001 tctggatctg tccgcgcgct tgagtggctc ggcgcaggcc attcgaggtt gcctgcaggc
     3061 gcggggcgcg agttttttca gcgagctgtt gcatgggacg cggttgctgg cctccgaagt
     3121 ggaagacggg ttgtgggaac tggttgcagc cggcctggtg accgccgacg ggttcgacaa
     3181 tctgcgttcg ctgatcgatc cgaagcggcg g
//
LOCUS       DAORRQ010000170         3123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_788_length_3123_cov_54.630378, whole genome shotgun sequence.
ACCESSION   DAORRQ010000170 DAORRQ010000000
VERSION     DAORRQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_788_length_3123_cov_54.630378"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1462)
                     /gene="hflX"
                     /locus_tag="PK224_21195"
     CDS             complement(74..1462)
                     /gene="hflX"
                     /locus_tag="PK224_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRB18321.1"
                     /translation="MSKPSQSNAVLVAIRTPRVTAEELDSSLQELTRLVTTLGYTMVG
                     RVTQKRSSDKYAAVLGQGKLAELAQWTGGSGKIESAFARPKHKGASKFEAEDSDVTEE
                     SDDDESDDTIEAAPGPGEQAQIVIVDCDLSPSQLKNLERAAGVPVLDRTGVIIEIFSR
                     HARTRAARLQVEIARLNYLAPRLRETGGGSERQGGGVGGKGAGETSLELDKRRIRDRT
                     KELREELAAIGDEHQTRRARREHELTVALVGYTNAGKSSLMRAMTGSEVLVADKLFAT
                     LDTTIRPLYPETCPKVLLSDTVGFIKKLPHDLVASFKSTLDEAASASLLLFVVDASDP
                     SFRSQLDVTRKVLAEVGATEVPSLLVLNKRDRLAPEELAALKAEYPDAIVLSTRNKDD
                     LQALRERIMGYFESDMLDEELRIPFTAQGVVAEIRAKMRILSEEYDTEGLTIRVRSTP
                     ENLTAIKKKLAR"
     gene            complement(1557..1955)
                     /locus_tag="PK224_21200"
     CDS             complement(1557..1955)
                     /locus_tag="PK224_21200"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRB18322.1"
                     /translation="MKPMTPAMLLRALTMFRGFTQTAAEYLRDKERLRHLLAAAVSIA
                     QGRGGKLLNDLQLLVRLLKASVSGAYTGFSVHKLVTIVAAILYLISPLDVIPDFIPVV
                     GYADDAAVIAWVLNSIAEELKDFKNWEQGT"
     gene            complement(2410..2622)
                     /locus_tag="PK224_21205"
     CDS             complement(2410..2622)
                     /locus_tag="PK224_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18323.1"
                     /translation="MAEKVKVWFDPEGDFLEVQFSDAPGFMRPTAYEAVMERVDGQGH
                     VLGFSVLGVSRFKKDHPLEAELMTGT"
     gene            complement(2607..2918)
                     /locus_tag="PK224_21210"
     CDS             complement(2607..2918)
                     /locus_tag="PK224_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HRB18324.1"
                     /translation="MKIIRDCFGRPVRLTDERSAHILRHQEMAGMEAEIERVLQSPAE
                     VRVSRSDHTVHLFYEFYGQTLVGGKWLCVVVKYPPDDGFVVTAYLTDQLKAGDTIWPK
                     K"
BASE COUNT          671 a          932 c          861 g          659 t
ORIGIN      
        1 ggcgggtcgc ggtcagtcag tcggcttgat tttcaagaca gttgctgacc ccaaataggg
       61 tggaatcagc gcctcatcgc gcgagcttct ttttgatcgc agtcaggttc tcaggggtcg
      121 agcgcacccg tatcgtgagt ccttcagtat catactcttc ggacaggata cgcatcttgg
      181 cgcggatctc tgcgacgacg ccttgagcgg taaagggaat gcgcaattcc tcgtcgagca
      241 tatcgctctc gaagtacccc atgatgcgct cgcggagcgc ctgcagatcg tccttgtttc
      301 tcgtggacag cacaatggcg tcgggatatt ccgccttcaa ggccgcgagt tcctccggcg
      361 cgagacgatc ccgtttattc aacaccaaca ggctgggaac ctctgtggcg ccgacttcgg
      421 ccaacacctt ccgcgtgacg tcgagttggg agcgaaagga cgggtcggag gcatcgacga
      481 caaacagcaa gagcgaagca ctggccgctt catccagcgt cgacttgaac gaggccacca
      541 ggtcatgcgg gagctttttg atgaaaccca ccgtatcgga caggaggacc ttcggacacg
      601 tctcaggata caagggccgg atcgtggtat cgagcgtggc gaacagcttg tcggccacga
      661 gcacttcact gcccgtcatc gcccgcatga gcgaggattt cccggcattg gtgtacccga
      721 cgagcgcgac cgtcaattcg tgttcccgcc tcgcacggcg cgtctgatgc tcgtccccga
      781 tcgccgccaa ttcctcccgg agttccttcg tgcgatcgcg gattctgcgc ttatcaagct
      841 ccagactcgt ctccccggct cctttgccgc cgactccccc gccctgccgc tcgctgccgc
      901 cgccggtttc ccgcagccgg ggcgcaagat aattgaggcg cgcgatctcc acctgcagcc
      961 gggccgctct ggttcgcgcg tgccggctga aaatctcaat gatgaccccg gtccgatcga
     1021 gcaccggcac gccggcagcc cgttcaagat ttttcaattg tgacggcgac agatcacaat
     1081 ccacgatgac aatctgcgcc tgttcgccag ggcctggagc agcctcgatg gtatcatccg
     1141 attcgtcgtc gtccgattct tccgtgacgt ccgaatcctc cgcctcgaac ttcgacgccc
     1201 ctttgtgctt cggccgtgca aacgccgatt cgattttccc ggacccaccg gtccattgcg
     1261 ccaattcggc aagtttgccc tgccccagga ccgcggcata tttgtcggag ctccgctttt
     1321 gcgtcacacg gcccaccatg gtgtatccga gggttgtcac cagacgggtg agctcttgca
     1381 gcgaactgtc cagctcctcc gcagtcacgc ggggggtgcg gatcgccaca agcacggcat
     1441 tcgattgtga gggcttcgac atcgcaggcc tttccttgat cacatcttcg tgagggtggc
     1501 cattctaaca gagaatttcg tgcaaaagcc tactcacgtc gcaggacttc ttccggtcac
     1561 gtgccctgtt cccaattctt aaaatccttc agttcttcag caatgctgtt cagcacccag
     1621 gcaatcaccg ccgcatcatc cgcatagccg accacaggaa tgaagtcggg gatcacatca
     1681 agcgggctga tgagatacag aatcgccgcc acgatggtga cgagcttgtg aaccgaaaag
     1741 ccggtatagg ctccgcttac ggacgctttc aggagccgca ccaataattg aaggtcgttc
     1801 aacagcttgc caccgcgccc ttgcgcgatc gataccgccg cggccaacag atgacgaagc
     1861 cgctctttgt ccctgaggta ctccgccgcg gtctgtgtga acccgcgaaa catggtcaac
     1921 gccctcagga gcatcgctgg agtcatcggc ttcatgaggt cctccctgag gggtgagcat
     1981 tgcactggca caccatcacg attgaggata acaggcagtc cgcgaaggat caatttggcc
     2041 cgatggcaaa ggacaagatc gaaccatcag cggcatggtg ggggcggaaa gtcggaggct
     2101 gtcgatggat gagatgccgt gggtgacggt gaacaagccg aaggcctcag cggtggggct
     2161 cacgggaaga ataaactccc gccaggtcag aatatttgac ccatgacctg agccccgtct
     2221 cctttcgtgt ctgttatgcc gggtcacctc agcctgcccc tgataggagc aatggctgcg
     2281 gacttctcat gttcggacga ttcgatagat agatagatag agagagagag agagagagaa
     2341 agattggtgg atgacactgg cgtagaaagc ggaagatgcc gtgcaattga tcgagtcagc
     2401 tctcacgcct tatgtgccgg tcatgagttc agcttcgagg ggatggtctt ttttaaaccg
     2461 gctgactccg aggacactga atcccaagac atgcccctgc ccatccaccc gttccatgac
     2521 ggcctcgtag gccgtgggac gcatgaaacc aggcgcgtcg ctaaactgca cttccaggaa
     2581 atcgccttca gggtcgaacc acacttttac tttttcggcc atatcgtgtc tcctgccttg
     2641 agttggtcag tcaagtaggc cgtcaccaca aacccgtcgt cgggaggata ctttaccacg
     2701 acacagagcc atttcccacc aaccagcgtc tgcccataaa actcataaaa tagatggaca
     2761 gtgtgatctg agcgagagac ccgcacttcg gccggcgatt ggagcacccg ttcgatctct
     2821 gcctccatac cagccatctc ttgatgccgg agaatatgag ccgaccgctc atcggtcagt
     2881 ctgacgggtc gaccgaaaca atcacggatg atcttcatct ctcactcgga atacgaggtt
     2941 tcaggaaatc cacaatcatc tgcaagccag gcattctaca actattaaag agaaagaaac
     3001 gactcggata caggcggagc gacctcttca gggaagcgat agagcgttcc ggagtgtggc
     3061 caggctgata ttcgattgca agaccttgta tctatagagg aggttttgca tcatggcacg
     3121 aca
//
LOCUS       DAORRQ010000171         3083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_794_length_3083_cov_16.290291, whole genome shotgun sequence.
ACCESSION   DAORRQ010000171 DAORRQ010000000
VERSION     DAORRQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_794_length_3083_cov_16.290291"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2524
                     /locus_tag="PK224_21215"
     CDS             <1..2524
                     /locus_tag="PK224_21215"
                     /inference="COORDINATES: protein motif:HMM:NF039490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18325.1"
                     /translation="GGTTLTSSGNHDITFNSTVNGARALAVNTTGTTTFGGSVGTTTA
                     LTSLTTDSGGTTALNGGAVTTSGNQTYNDAVTMNQFTTVTSTGGAITFAEHATNTLAG
                     AGLTVEAPTLSLTSGKTVATTGSGPIRFLTNSFNPNGANIDAGTGAFTLSPTTLTNTI
                     EFGDVNTARATTVYYGSLFGNLTAGSFTIGRPTHSGNIFVTGVAAAPSSIQIVNGGAG
                     AVTFEHAPYVGGNQSLGVTGGTGGITIGQDMTLGTGTLRLTTTGAISQTAGTLIAETA
                     GVSAASGITFAQPLNDVATLAARTAAGNLTFTNNNGFTIGAVTATADGFHPAVTGVSA
                     GGAITLQSGGAVTQTQRILGSSLRLQGTGPFTLTDNANEVTAFSANTTDHVQYTDASD
                     VILGTSNIPGHFDLTTSGAITQSGALTVTGRTTLAAGSGDITLTQAGNNFSRLDVTSA
                     NHVALTDSDALVLGASTVNGTLDITTNGALTQSGALTVGGATTLASGSYDITLIDAGN
                     DFTSVSITGGNHVSLRDTNALRLAASTINGNLHADAGTLTIGGALTSSGGNLTLTGTN
                     SVTQLAHLSVTGAHTVTVTAPSGPLAMAPTATTSSDTGTIAYAAGADITLGSLRTGAG
                     VTVISSGGSVLSAAGSGMNVTAGANSSLQALNGVVGTQVAPITVHVSAGTLGIHATAA
                     RFGISAFLNGTVFPGQALTMLNAPPRPCLFQCMPVQHDSLIQRGVRNPLVYAPRIQPP
                     VVQHSLDLSAGRRSRRNSYGRVLRRRPRGARDPSLHPGRRLRTQSRSPHATLKHGRPN
                     SVLIGDSSPDHLASPIKHRRTNLRSSAPKPSATTAAARPPTG"
     gene            complement(2442..2975)
                     /locus_tag="PK224_21220"
     CDS             complement(2442..2975)
                     /locus_tag="PK224_21220"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HRB18326.1"
                     /translation="MNGAIANAVVFQVGWWIAVLGTGWAYHWIGPLLVACLVCVNVCS
                     SADRRATARIVIVVGLFGTLLDSALSFAGLLLFVENPFAPWLCPPWLIALWCLLATTL
                     NGSLRWLAGRNRSAALVGGIFGPLSYYAGQQLGALRLGRNETLSLLLLSILWAVVLPL
                     LLRWASGRRSAGLSGDA"
BASE COUNT          679 a         1075 c          869 g          460 t
ORIGIN      
        1 cggcggcacc acattgacaa gctccggcaa tcacgacatc acgttcaaca gcaccgtcaa
       61 cggagcgcgg gctctggccg tcaatacgac cggcacgact accttcggcg gttcggtcgg
      121 aaccaccacc gcgctcacga gcttgaccac cgactccggc ggcaccaccg ctcttaacgg
      181 cggagcggtc accacatcag gcaaccagac ctacaacgat gccgtgacca tgaaccagtt
      241 cacaaccgtc acctccaccg gcggggccat cacgtttgcg gaacacgcca ccaataccct
      301 ggcgggagcc ggcctgacgg tcgaggcacc caccctttcg ctcacaagcg gaaagacggt
      361 ggccacgacc ggcagcggcc cgatcagatt cctgacaaac agctttaatc ccaacggagc
      421 gaatatcgac gcgggaacgg gcgcgttcac actgtcgccg acgaccctga cgaacaccat
      481 cgagttcggc gatgtaaaca ctgcgcgggc gaccacggtg tattacggat ccctgttcgg
      541 aaatctgacg gccggcagct ttaccattgg acgcccgacc cacagcggca acatcttcgt
      601 tacaggcgtg gcggcagccc ccagttcgat ccagatcgtg aacggcggag cgggagcggt
      661 tacctttgaa cacgccccct atgtcggcgg aaaccaatcc ctcggagtca ccggcggaac
      721 cggcggcatc accattgggc aagacatgac gcttgggacc gggaccttgc ggttgacgac
      781 gacgggagcg atttcgcaaa cggcaggaac cctcatcgcc gagaccgccg gggtctccgc
      841 ggcaagcggg atcacgtttg cacagcccct caatgacgtt gccacactgg cggctcggac
      901 ggcggcaggc aacctgacct tcacgaataa caacggattc acgatcggcg cagtgacagc
      961 taccgcagac gggttccatc ccgctgtcac aggtgtgagc gcgggcggcg ccattactct
     1021 tcagagcggc ggcgcagtca cgcaaaccca gcggattctc ggctcaagct tgagacttca
     1081 aggcactggt cctttcacgc tgacggacaa cgcaaatgag gtgacggcat tctcagccaa
     1141 tacgaccgac cacgtacaat acaccgatgc gagcgacgtc attctcggca cctcgaatat
     1201 cccgggtcac ttcgatctca ctacgagcgg cgccatcacc cagagcgggg ctctcaccgt
     1261 caccggcaga acgacactcg cggcgggatc gggcgacatc acactcacac aggccgggaa
     1321 caatttttcg cgcctcgacg tcacaagcgc gaaccacgtc gccctgacgg acagcgatgc
     1381 gctggtgctc ggcgcctcaa ccgtcaacgg caccctggac atcaccacca acggcgcact
     1441 cacccaaagt ggggcattga ccgtgggcgg cgccaccacg ctcgcctccg gctcctatga
     1501 catcaccttg atcgatgccg gaaacgactt caccagcgtc agcatcaccg gcggcaacca
     1561 cgtctcgctc agggacacga atgcgctcag gctcgccgca tcaaccatca acgggaacct
     1621 gcacgccgat gccggcactc tgacgatcgg gggcgcgctg acgtcaagtg gcggcaacct
     1681 gaccctgacc ggcacgaact cggtgacgca gctggcccac ctttcggtaa ccggcgccca
     1741 cacggtcacg gtcactgcgc ccagcggccc gttggccatg gcgcccaccg ccacgaccag
     1801 cagcgacacc ggcaccatcg cctacgccgc aggagcagat atcaccctgg ggtccctgcg
     1861 caccggtgcg ggagtgactg tgatatcgag cggcgggtcg gtgttgagcg cagcaggttc
     1921 gggcatgaac gtcaccgcag gcgcgaactc atccttgcag gcgctaaacg gagtggtcgg
     1981 cacgcaagtc gcgccgatca ccgtgcatgt gagcgccggc accctcggca ttcatgcgac
     2041 ggcggcccgt ttcggcatct cggcctttct gaacggcacc gtgttccccg gccaggcgct
     2101 caccatgctg aatgcccccc cccggccttg tctgtttcaa tgcatgccgg ttcagcacga
     2161 ttccctcatt caacgtggcg tccgcaatcc cctggtatat gcgccacgca tccaaccccc
     2221 tgtggtacag cattctctcg acctatctgc cggaagacgt agtcgaagga actcctatgg
     2281 acgtgtactt cgacgaagac cgcgtggcgc gcgagatccc tccttgcacc ccggccggcg
     2341 cctgcgcacc caaagccgca gtcctcacgc caccctcaag cacggaagac ccaacagcgt
     2401 actgatcggt gattcatcgc ctgaccatct agcatcgccc atcaagcatc gccggacaaa
     2461 cctgcgctcc tccgccccga agcccagcgc aacaacagcg gcagcacgac cgcccacagg
     2521 atagacagca gcaacaacga gagggtctca ttgcggccaa gccggagcgc tccgagttgt
     2581 tgtcctgcgt agtaactcaa cggaccgaag atccccccga caagcgccgc cgaccgattg
     2641 cgccccgcca accaacgaag agacccgttc aacgtcgtcg ccaagaggca ccagagcgcg
     2701 atcaaccagg gcggacacag ccagggcgcg aaggggttct cgacaaatag gaggagaccg
     2761 gcgaagctga gggcgctgtc cagcagcgtt ccgaacaatc caactacgat gacgattcga
     2821 gcggtcgcgc ggcgatcggc cgaggagcag acattcacac agaccagaca ggcgactagg
     2881 agcgggccga tccagtgata ggcccaaccc gttccaagga cagcgatcca ccagccgacc
     2941 tgaaagacca ccgcattggc gatggcgccg ttcatcctgc caggagatcc tgccgccacc
     3001 cggatttcgc gaacaggagg tgggccgtcg aaatcgaccg ttcgaggaat ccgccttcgc
     3061 agtaggataa ataaaaatcc cac
//
LOCUS       DAORRQ010000172         3001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_812_length_3001_cov_163.032247, whole genome shotgun sequence.
ACCESSION   DAORRQ010000172 DAORRQ010000000
VERSION     DAORRQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_812_length_3001_cov_163.032247"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..826)
                     /gene="rnpB"
                     /locus_tag="PK224_21225"
     ncRNA           complement(402..826)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK224_21225"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(887..1777)
                     /locus_tag="PK224_21230"
     CDS             complement(887..1777)
                     /locus_tag="PK224_21230"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRB18327.1"
                     /translation="MEAQQKMVRRRTSMMRSNMLCLAGVVVAMLVTTGCQTNPYTGRW
                     QLMMMPMSQETQMGAQAYAEVKNDPKMKTSTDPREIEPVKRVAARVIEAAKRSKYSEM
                     ANQFEWEVTVIKDDKTMNAFALPGGKIAVYTGIFPVAKTEAGLAAVMGHEVVHALARH
                     GGERMSQNTLAQTTLQAIGIALGVSGANPVVSQGAMAALGVGAQVGVLLPFSRKHESE
                     ADYVGVLLAADAGYDPRESIQLWTRMGELSGGKSASEFMSTHPSHETRIEQLEEWMSE
                     AMPIYQSKPPAPNHELPALR"
     gene            1864..2523
                     /gene="rnhC"
                     /locus_tag="PK224_21235"
     CDS             1864..2523
                     /gene="rnhC"
                     /locus_tag="PK224_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HRB18328.1"
                     /translation="MPAATSLSSLDRIGIDESGKGDYFGPLVIAAVFVTPALEQDLAL
                     MQVRDSKKISDGRILELAPDIRLLCPHSLVAIGPQRYNELYAKIKNLNRLLAWGHARA
                     LENLLQQVECDLAIADQFGDERLILNALQEKGKQIRLVQRTKAESDLAVAAASILARA
                     EFLQRLDRLSQELNTTLPKGASPAVELAGRMVVKKYGRDRLGTVAKLHFKTTKQVLGE
                     T"
     gene            complement(2520..>3001)
                     /locus_tag="PK224_21240"
     CDS             complement(2520..>3001)
                     /locus_tag="PK224_21240"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRB18329.1"
                     /translation="ANKKRGEIEEKILLAMEQIEQTQRTIVDVQAKLKESEALFIKEK
                     AKLDEKDRLLATELAELEVKQKHLSGQVEKSLLARYNKLKTTRKEQALALIKDGICVG
                     CRLQLPPQLVSQVKRGEDVHTCPYCYRMLYWEGDPATEAKGTVDHDQAKNLEVGESF"
BASE COUNT          578 a          953 c          806 g          664 t
ORIGIN      
        1 aatctttctg gaaaatcaac gggtaagatg gcggatcgta tcaccagctc tcaagtgagg
       61 tcaagagagc cgatgcacga attcttcgcc ggggagactg gtgccggctc atgccccgtg
      121 atatagggga gtcagccaac ccattgatcg tgctgcctat gaatcctcta cgcgtcacca
      181 tcatgtcccg ccacgagtgc catctttgtc gagtggtcac acgggtggtt ggacaagtgc
      241 aacaagacct tccctttgag ctcatcacag tcgatatcga cagtgacgag cgcctccgca
      301 aacagtacag cgaccgcgtg ccggtgctgt tgatcaatga ccgggaaacg ttctccggca
      361 aggtgaccgc cggagagctg cgtgaagcga ttaaaaaagc gcgttggaga aaccctataa
      421 gccggattct gtcccgcgtg aagcttgcgc tcacacgggg gtgatcattt ctctgggact
      481 cgagttacct cgagcctcaa gcaacctacc cgggaacttc ggccgggccg gcctttctgc
      541 cgagctctcg cgagcccaca ggcgtccccc tatttggtct tgctccggac gacgcttacc
      601 gtgccgttga tgtcgccatc accgcggtgg gctcttaccc caccgtttca cccttacctg
      661 agccggcgca ctcgaccgaa gtctcccgca ctggccatcg gcggtttgtt ctctgtggtg
      721 ctggtgtcgg atcgctccgc ctggacgtta tccagcgtcc ttgcccttgg agtccggact
      781 ttcctcccgt caagcacgct cgacgagcga gcacccgtgt ccccccagcg cgcaacgaaa
      841 gtatacgagc cgcccgatag ggattcaagc ggcgagccct ccggcactaa cgcaacgctg
      901 gaagctcgtg gttgggcgcc ggtggcttcg actgatagat cggcatggcc tctgacatcc
      961 attcctccag ctgctcgatt ctagtctcgt ggctagggtg ggtcgacatg aattccgacg
     1021 ccgatttgcc gccggacagt tcgcccattc gcgtccaaag ctgaatcgat tcccgtggat
     1081 cgtacccggc atccgctgcg agcaacaccc cgacatagtc ggcctcggac tcatgtttcc
     1141 ggctgaacgg gagcaacacc ccgacttgtg caccgacacc caatgctgcc atcgctcctt
     1201 gcgaaaccac cggattggcg ccactcacgc cgagcgcaat tccgatcgcc tgcaaggtcg
     1261 tctgcgccaa ggtattctga ctcatccgtt ccccgccgtg ccgtgccaac gcgtgcacaa
     1321 cctcatggcc catcacggcc gcaagtccgg cctcggtctt ggccaccgga aaaataccgg
     1381 tgtagacggc gatctttccg cccggcagcg caaacgcgtt catcgtcttg tcgtccttga
     1441 tgaccgtcac ttcccactcg aattgattcg ccatctcgct gtacttagaa cgcttggctg
     1501 cttcgatcac ccgcgccgcc acccgtttca ccggttcaat ctcccgcggg tcggtcgacg
     1561 ttttcatctt cggatcattc ttcacttcgg cataagcctg ggcgcccatc tgcgtttcct
     1621 gagacatcgg catcatcatg agctgccagc gtccggtgta ggggttggtt tggcagcctg
     1681 tggtcaccag catggccacg acaacaccgg cgaggcacag catgttcgac cgcatcatgg
     1741 acgtccgtct tctgaccatc ttctgttgag cctccatcac acgcctccgc aaattgacca
     1801 gtccggtgag ctctgctaga ttaccgtgcc gcccggccag ttgctacatg ggagtttcac
     1861 cgagtgcctg ccgccaccag cctctcatcg ctcgatcgca tcggaatcga cgagtccggc
     1921 aagggggact atttcggtcc gttagtgatc gccgctgtgt tcgtcacacc ggccttggag
     1981 caggacttag ccctgatgca ggtccgggac agcaagaaaa tctccgatgg gcggatcctc
     2041 gaactggcgc ccgacatccg cctgctctgc ccccatagtc tcgtggccat cgggccgcag
     2101 cgatacaacg agctgtatgc caagatcaag aacttgaatc gcctcctggc ttggggacat
     2161 gcccgtgccc tggaaaatct gctccagcaa gtcgagtgcg acctcgcgat tgcagaccag
     2221 ttcggggacg agcgattgat cttgaacgca ctgcaagaaa agggaaaaca gatccggctg
     2281 gttcaacgta cgaaagcgga atccgatcta gctgtggcag cggcctcaat cctggctcga
     2341 gcggagtttc ttcagcgcct cgatcgactc tctcaagagc tgaacacgac gctacccaag
     2401 ggcgcatcac ccgccgtgga actggccggg cggatggtgg tcaagaaata cggacgtgac
     2461 cggctaggga ccgtcgccaa gctgcacttc aaaacaacga agcaggtcct gggagaaact
     2521 tagaaggatt caccgacttc caagttcttc gcttgatcgt gatccacagt ccccttcgcc
     2581 tcggtagccg ggtccccttc ccaatacagc atccgatagc aataggggca ggtatggaca
     2641 tcttccccgc gtttgacctg cgagaccagc tgcggcggca attgaagacg gcaccctacg
     2701 caaatgccgt ccttgatgag tgccagcgcc tgctctttcc ttgtagtttt cagtttgttg
     2761 tatcgggcca ggagtgattt ttcgacttgt cccgacaggt gcttttgctt gacctctagt
     2821 tcggccagct cggttgcaag cagccggtcc ttctcgtcca gctttgcctt ctctttgatg
     2881 aagagggctt cggattcctt cagctttgcc tgcacatcga cgatggttcg ctgcgtctgc
     2941 tcgatctgct ccatcgccag cagaattttt tcttcaatct cgcccctctt cttattggca
     3001 a
//
LOCUS       DAORRQ010000173         2991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_816_length_2991_cov_147.985014, whole genome shotgun sequence.
ACCESSION   DAORRQ010000173 DAORRQ010000000
VERSION     DAORRQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_816_length_2991_cov_147.985014"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..697
                     /locus_tag="PK224_21245"
     CDS             107..697
                     /locus_tag="PK224_21245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18330.1"
                     /translation="MGVTHLVRTGLVAMAVVALVLLPKADDIMATLERTGSLNANVLE
                     RLAWLMDPSGVSDASSWSRKYVAQQAWERVGEQPFLGGGTGAVHERLDIPPHNQFLAY
                     MMDHGLLGAMLLPLLLLALLWRAQEESLRVGLIFCCAVLWFSFFTHTMLNNSHSLLLF
                     SLMAALASTRFQRMDRVVVSRTVAEQPLPTLARTHS"
     gene            735..2012
                     /locus_tag="PK224_21250"
     CDS             735..2012
                     /locus_tag="PK224_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRB18331.1"
                     /translation="MRNLFLVGIGLLMGVALCELGLRLAGVSYPIFSKIDGDLGVVLR
                     PDAEGWWKTEGEAYVRINGDGLRDRDHSRSKPADVLRIAVLGDSFAEAVQVKTEDTFW
                     AVLEHDLRGCPALGGREPEVINFGVSGYGTAQELIMLRRRVWAYSPDVVLLAVTPTND
                     IRNNFKALEKDERRPYFVRDGGTLLEDTSFRERLGFRLRFSSVGQALTSVRDASRLFQ
                     LANEALRRVSRPIRPVHTLPPSEGRKGGDHDEVKQARDIPARLQGEVGLDMATYTAPP
                     DETWQEAWLVTEALLRQMHAEVEARGKLFMVLTVSSGPQVRPEQAERRSLEQRLGVSD
                     LFYAENRLRQLGEQRFPVVTLAPVFQAYAEERKVFLHGFANSGLGQGHWNAAGHRLAG
                     QVVAERLCRALADSRAHQAAGATLASGAAYQAP"
     gene            complement(2045..>2991)
                     /locus_tag="PK224_21255"
     CDS             complement(2045..>2991)
                     /locus_tag="PK224_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18332.1"
                     /translation="DAVNFTAGPGFILSSPPAGLQEYGLSWSGVSPTLTYGIGGHKIW
                     EINVATQQATLVKDLSSQGAGGYLTQMLKSLDDDVFGASIVDGSGNIAGYVVYKRSTD
                     TILLRKLVSNLDEIQVDKSGRYLLVVYNNGTDEVYDLTTSPPRFVAYLSGSSGFNHHD
                     CGVGTVFAATSGNALSYRQLATPFTIQQMVPGYWSYSGQQDHFSMLADNEEWAMASRY
                     STNGSPAVRAFDNEIVKVATDGTNRVQRIAHHRSVVLNNNYNAQPKAAISRDGNLIAF
                     TSNWGNSSGRTDVYVVQIESGIMADNTAPNIPRNLVVR"
BASE COUNT          546 a          773 c          977 g          695 t
ORIGIN      
        1 ggtttttttg gggggccccc gggcgggggg gggtttgggg cggtgcgcgg ggggggtttg
       61 gggggggggg gggattggtc tgggtggatt gctgctgggg aggttcatgg gcgtgacgca
      121 tttagtgcgg accggtctgg tggccatggc agtcgtcgcc ctcgtccttc tgccgaaggc
      181 cgatgacatc atggcgacgc ttgagcgaac cggatcgttg aatgccaatg tgctcgagcg
      241 gttggcatgg ctgatggatc cgtccggcgt gtcggatgca tcgagttggt cgcggaaata
      301 tgtcgcacaa caggcctggg aacgggtggg agaacagccg ttcctgggtg ggggaaccgg
      361 ggcggttcat gaacggctcg atattccacc gcataaccaa ttcctggcct acatgatgga
      421 tcacggcctg ttgggagcca tgctgctacc actgttgttg ctggccttgc tctggagagc
      481 gcaggaggag agtctgcgcg tggggctgat tttctgttgc gcggtgttat ggttcagctt
      541 ttttacccat acgatgctga acaattctca cagcctactg ctgttttcgt tgatggccgc
      601 gctggcttcg acgaggtttc agcggatgga tcgcgtagtc gtcagcagga cggtcgccga
      661 gcagccattg cccaccttgg cgagaactca ttcgtagcct gcgcgttcaa ggtgaatctc
      721 acgcgatgat ccgtatgcgc aatctctttc ttgtcgggat cggtcttttg atgggcgtcg
      781 ccttgtgtga actgggtctt cgtctggcgg gagtgtccta tccgatattt tccaagatcg
      841 acggtgattt gggcgtggtg ttgcggccgg atgccgaagg atggtggaaa acggaaggag
      901 aagcctatgt gcgtattaac ggcgacggtc tgcgagatcg cgaccattct cgatcgaagc
      961 ctgccgatgt gctccggatc gcggtgttgg gagattcgtt tgccgaagcc gttcaggtaa
     1021 agaccgagga caccttttgg gctgtgttgg agcatgatct ccgtgggtgc cccgctcttg
     1081 gagggcgcga accggaggtg atcaattttg gggtctccgg gtatggcacg gcacaagagc
     1141 ttataatgtt gcgtcgccga gtatgggcct attcgccgga tgtggtgctt ttagccgtga
     1201 ccccgacgaa tgatattcgg aacaacttta aggcgttgga gaaagatgag cggcgcccct
     1261 attttgtccg tgacggtgga acgctgctgg aagacacctc cttccgagag cggcttgggt
     1321 ttcgtcttcg cttctcgtcg gtcggacaag ccctgacgtc cgttcgtgat gcgtcccgtc
     1381 ttttccaatt ggcgaacgag gcacttcggc gcgtcagccg gccgatccgt ccggtccaca
     1441 cgcttccgcc ctccgaaggg cgaaaaggcg gagaccatga cgaggttaaa caggcgaggg
     1501 atataccggc gcggttgcag ggggaggtag ggttggacat ggcaacctat accgctccgc
     1561 cggatgagac atggcaagag gcatggttgg tgacggaagc cctcttgcgt caaatgcacg
     1621 cggaagtcga agcgcgtgga aaattgttca tggtgctgac agtcagcagt ggcccgcagg
     1681 tcaggccgga gcaggccgaa cggcggtcgt tggagcagcg gctaggcgtg tcggatctgt
     1741 tttatgccga aaatcgtttg cggcagcttg gtgaacaacg ctttcccgtc gtaaccctcg
     1801 cgccggtttt tcaagcttac gcggaggagc gcaaggtctt tctccatggt tttgcgaatt
     1861 caggccttgg ccagggccac tggaatgcgg ccggccatcg gctggccggt caggtggttg
     1921 cagagcgact ctgtcgcgcc cttgcagact cgcgagccca tcaggctgcg ggggctacgc
     1981 ttgcgtcagg cgctgcctat caggcgccct agtctcggat gtctgcgccc gtacagcgcc
     2041 ctgtttaccg gacgacaaga ttgcggggaa tgttgggagc agtgttatcg gccatgatgc
     2101 cgctctcaat ctgtacgaca tagacgtctg ttcgcccgct agagttgccc caattgctgg
     2161 tgaaggcgat taaattccca tcgcggctga tcgccgcctt gggttgtgcg ttgtagttgt
     2221 tgttcagcac gacgctgcgg tggtgagcga tgcgctgcac ccgattggtc ccgtcggtgg
     2281 ccacttttac gatctcattg tcaaatgccc gcactgcggg gcttccgttt gtcgaatagc
     2341 ggctggccat ggcccattct tcgttgtcgg ccaacattga aaagtgatcc tgctgcccgg
     2401 aataggacca gtaaccagga accatttgtt ggatcgtgaa gggtgtcgcc aactgacggt
     2461 agctcagggc gtttcctgag gtggcggcaa agacagtccc gactccgcag tcatggtggt
     2521 taaagccgct cgaaccgctg aggtaagcca cgaaacgagg cgggctagtg gtcaaatcat
     2581 acacctcatc cgtgccattg ttgtaaacca ccagcagata ccggcctgac ttatctacct
     2641 gtatctcgtc aaggttgctg accaacttgc ggagcaggat ggtatcggtg ctccgtttgt
     2701 agacaacgta gcctgcgatg tttccactcc cgtcgacgat cgaggcccca aagacgtcat
     2761 cgtcgagact cttgagcatt tgagtgaggt accctcccgc accctggctg ctcagatcct
     2821 tgaccaatgt agcctgttgg gtcgctacgt tgatttccca aatcttgtgg ccgcctatgc
     2881 cgtaggtgag ggtaggagag accccggacc aagacaagcc atattcctgc aatccggccg
     2941 gtggagaact caggataaag cccggaccag ccgtgaaatt gacggcatcg a
//
LOCUS       DAORRQ010000174         2976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_823_length_2976_cov_14.824718, whole genome shotgun sequence.
ACCESSION   DAORRQ010000174 DAORRQ010000000
VERSION     DAORRQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_2976_cov_14.824718"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK224_21260"
     CDS             complement(<1..505)
                     /locus_tag="PK224_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRB18333.1"
                     /translation="MMREQDAPTEPVDAPLVSASVTLPEITLKAVVLSILLAAVLAGA
                     NAYLGLFAGMTVSASIPAAVVSMAVLRLFRQSNILENNIVQTAASSGEALAAGVIFTI
                     PGLVLIGYWTTFDYWQTFTVSLVGGVLGVLFTIPLRRALIIHARLRFPEGVATAEVLK
                     VGASSDAG"
     gene            complement(510..1706)
                     /locus_tag="PK224_21265"
     CDS             complement(510..1706)
                     /locus_tag="PK224_21265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRB18334.1"
                     /translation="MTQLVTGSTAKVRLTAEREGPRYYGHLWVFDSNVADVLGTPAAG
                     DLVDVYTHQKRFFGRGLFNPHSKIRIRMLTFQEEPIDEEFFAARLRAAAALRRTVAPH
                     ATACRLVHGESDLLPGLVVDRFADVAVMQTLGYGMDVRKELLGELLVQEAGVKMVYLR
                     NDAKSRTLEGLPLSKGFLRGEGATTVNIHEGKAQFTVDFAEGQKTGWFCDQRENRIAA
                     ALFAKGKTVLEAFCHTGGFGIQAALAGAQSVEGLDVSAAAVALAQAHAEQNQVAARCQ
                     YRQADAFEELRLLERNGQRYDLVILDPPAFARSKKAVPHAIAGYKEVNLRGLRLLQPG
                     GVLVTCSCSQPITDEDFWKMLQAAARDARRQIRLLEQRGQGPDHPVLAGMPETRYLKC
                     YLVQVF"
     gene            1929..>2976
                     /locus_tag="PK224_21270"
     CDS             1929..>2976
                     /locus_tag="PK224_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRB18335.1"
                     /translation="MTIQTIGTAVLDRLHRLGVRHMFGIPGDYVLGLYKLMESSPIQH
                     VATTREDCAGFAADAYARINGIGALCVTYCVGGLNTVNAIACAYAERSPVVLLTGSPG
                     LSERARNPYLHHMVREFSTQREVFEKMTVAAVSLEDPVTAEREMDRAFAALLRYRRPI
                     YLEIPRDMVHVPLAQTSHKHQTQGEPTDRAALSEALNEVRTMLESAKRPVILAGAEVG
                     RFGLHDELTSLVERLNVPIASTLLGKSIIREDHPLYVGVYSGLVARDEVKEFVNQTDC
                     LLILGSILSDVEDLDAHSALFSDGHTIHATADRVAIKHHRYDSILFEDFVKGLAAASL
                     PSFPTPQLPAPYKPE"
BASE COUNT          602 a          989 c          845 g          540 t
ORIGIN      
        1 caccggcatc cgacgaggct ccgaccttca ggacttccgc cgtcgcaacc ccttccggga
       61 aacggagccg ggcatggata atcagggcac ggcgcagggg aatggtgaac aacaccccca
      121 gcaccccgcc gacgagcgac accgtgaacg tctgccaata gtcgaaggtg gtccagtagc
      181 cgatcaggac cagccccgga atggtaaaga tcaccccggc cgccaacgct tcgcctgagg
      241 aggcggcggt ttgcacgata ttgttttcca gaatattcga ctgacggaag agacgcagca
      301 ccgccatgga gacgacggcg gctggaatgg aggcggacac cgtcatgccg gcaaacaggc
      361 ccaaataggc gttggctccg gccagcacgg cggccagcag aatcgacagg acgaccgctt
      421 tcagtgtgat ttccggcagc gtgaccgagg cggaaacgag cggcgcgtct accggctccg
      481 ttggcgcatc ctgctcgcgc atcatcccct cagaacacct gcacgaggta gcatttgaga
      541 tagcgcgttt ccggcatgcc cgcaagcacc ggatggtccg gaccctgtcc acgttgttca
      601 aggagcctga tctgccggcg cgcatcccga gcggccgcct gcagcatctt ccagaagtcc
      661 tcgtccgtga tcggctgcga gcaggaacag gtcaccagca cgccccccgg ctgaagcaga
      721 cgaaggccgc gcaaattcac ttccttgtac ccggcgatgg catgcggcac cgccttcttg
      781 ctacgcgcga acgccggtgg atcgagaatg accaggtcgt accgttggcc gttacgctcc
      841 aacaggcgca actcttcaaa ggcatcggcc tggcgatact gacaacgggc ggccacctga
      901 ttctgctccg catgcgcctg ggccagcgcc acggccgccg cgctcacatc caacccttcc
      961 accgactgcg cccccgccaa cgcagcctgt atgccgaatc cacccgtgtg gcaaaaggct
     1021 tctaacaccg ttttcccttt tgcaaagagg gctgcggcga tccggttttc ccgttgatcg
     1081 cagaaccagc cggtcttctg cccctccgca aaatcgaccg taaactgggc cttcccctcg
     1141 tgaatgttta cggtcgtggc accctcaccg cgcaggaacc ctttcgacaa cggcagccct
     1201 tccagcgtcc ggctcttggc atcattgcgg aggtacacca tcttgacgcc cgcttcctgc
     1261 accagcaact cgcccaacag ttcctttcgc acatccatgc cgtaccccag ggtctgcatc
     1321 accgccacat cggcaaaccg gtccacgacc aggcccggca ggagatcgct ttcaccatgc
     1381 acgaggcggc aggccgtggc atggggcgcg accgtccggc gaagggccgc cgcggcacgg
     1441 agacgcgcgg caaaaaactc ctcgtcgatc ggctcctcct gaaacgtgag catacggatg
     1501 cgaatcttgg aatggggatt gaagaggccg cggccgaaaa accgtttctg atgggtgtac
     1561 acatcgacga ggtccccggc agccggtgtg cccagcacgt cggccacatt gctgtcgaac
     1621 acccacaggt gcccgtagta acgcggccct tcacgttcgg ctgtcagtcg gactttcgca
     1681 gtagaaccgg tcacaagctg tgtcatggta atggttacgt cccgtataag ctgtttgatt
     1741 cgacgatggt gaaatggctc acgagggtgc ctgaaataat cccgcgaagc aaccggacag
     1801 tgggcgggca gcatgccgat gtgacgatcg ctcctgtgct tgaccttccc tagcacctat
     1861 ggtttgctgc aggaagacgg gcggacagta cgcaaattca cgacctctca ctcgacagcc
     1921 gaggcaccat gaccatacag acgatcggaa cagcagtcct ggatcggctc caccggctcg
     1981 gagtccgtca catgttcggc atccccggcg actacgtcct cggcctttac aaattgatgg
     2041 agtcttctcc catccaacac gtggccacca cgcgggaaga ctgtgcgggg tttgcggccg
     2101 acgcctatgc gcgcatcaac gggatcggcg ccctctgtgt cacctactgt gtcggcggac
     2161 tcaataccgt caacgccatc gcctgcgcct atgcggaacg atctcctgtc gtgttgctga
     2221 ccggctcccc cggattgtcg gaacgtgccc gcaaccccta cctgcaccac atggtgcgcg
     2281 agttttcgac gcagcgggaa gtcttcgaaa aaatgaccgt ggcggccgta agcctggaag
     2341 accccgtcac cgccgaacgt gagatggatc gcgccttcgc ggccctgctg cggtatcgcc
     2401 gcccgatcta tctcgaaatt ccgcgcgaca tggtgcacgt gccgttggcc cagacgtcgc
     2461 acaaacatca gacccaaggc gaaccgaccg accgcgcagc gctgagcgaa gctttgaatg
     2521 aagtacgtac catgttggag tcggccaaac gcccggtgat tctggccggc gccgaagtgg
     2581 gccgcttcgg gctccatgac gaattgacga gcctggtcga gcgcctgaat gtgccgattg
     2641 cttcgacgct cctcggcaag tccatcattc gagaagatca tccgctctat gtcggcgtct
     2701 acagcggact cgtcgcccgc gatgaagtga aggaattcgt caaccagacc gattgcctgc
     2761 tcatcctggg ctccatcctg tccgacgtgg aggacctgga tgcgcacagc gccctctttt
     2821 ctgacggcca taccatccat gccaccgcag atcgtgtcgc catcaagcat caccggtatg
     2881 actcgattct gttcgaggat ttcgtcaagg gactggccgc ggcctcactc ccttcatttc
     2941 caaccccgca attgccggct ccgtacaagc ccgaag
//
LOCUS       DAORRQ010000175         2756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_871_length_2756_cov_5.423547, whole genome shotgun sequence.
ACCESSION   DAORRQ010000175 DAORRQ010000000
VERSION     DAORRQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_871_length_2756_cov_5.423547"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PK224_21275"
     CDS             <1..664
                     /locus_tag="PK224_21275"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRB18336.1"
                     /translation="SMPVQTAQAIQEVNRTVQEPVETVVEPVQAMMRTEAAVEQVAVV
                     EQQTPAVQQTPTAAPVMRQAEPVTQVATVVSDAPAVEAAPAPVEAPQQVEQAIVQRPV
                     ETVQHRIIQQRTVQSRPGERPDYGWLSQVLWSSVDQRKRYPAEAKRNHWEGRVILRLT
                     LEQRGAAVHLLDLVLEESSGHTILDRHTLDMVRNAFPLQVKHQLAQSKLQVDLPFSYR
                     ME"
     gene            698..1153
                     /locus_tag="PK224_21280"
     CDS             698..1153
                     /locus_tag="PK224_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18337.1"
                     /translation="MLSHCHSLRSTVATVATTAALICGTALPAAAQQNGDKPFHALVT
                     DAKDMQTDLKNVLFYWEEKVSETSFIPHELKHLPVKRGTATVNIKFDGVKQVEVTPGA
                     DKALPTLHITLTNGKTGDFPLAIDGSFKGDSDFGQVELPVHGLKKLEFK"
     gene            1237..2316
                     /locus_tag="PK224_21285"
     CDS             1237..2316
                     /locus_tag="PK224_21285"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB18338.1"
                     /translation="MTLRRTTYLVIFTLLALSAMLAVKWTVLTPVPASEVTGTVPAGT
                     AVVLAPVTTEAIRHTIKAVGSLRANESIMIRPEIAGRIRQVWFQEGQAVEKDQLLIEM
                     DDSELQAELAQAAAQLKVSRLTYERLKRLDLDGKRYVTKQQLDEVAGALQVSQANHAL
                     YVTRLAKTKIRVPFTGVTGIRRVSPGDFVGSGRDLVNLEDLDTLKIDFKVPETMLRHL
                     APGQSLELTTDAYPGNTFHGTVYVIDPQVDPSTRSVQVRARIPNPQLRLRPGMFAQVL
                     LTFGQEERALLIPEEAVMPKQDKAYVYLAQDGTAQQREVTLGTRTRGFVQVLDGLTEQ
                     DTVIRVGHHKLQNGDSILALSSTPH"
     gene            2339..>2756
                     /locus_tag="PK224_21290"
     CDS             2339..>2756
                     /locus_tag="PK224_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB18339.1"
                     /translation="MRLSALSIERPVLAAVATLLLMLFGLLSYSRLPVREYPDTTFPI
                     VSVMTVYPSADAQLVETDITSVLEDALSGVEGLRTLRSTSREGLSTIGLEFSLTRNLD
                     AAANDVRDRVTRVQYLLPQGIEAPLVMKEDSEADGIM"
BASE COUNT          593 a          906 c          799 g          458 t
ORIGIN      
        1 gagcatgccc gtgcaaaccg ctcaggccat tcaagaggtc aaccgcacgg tccaggagcc
       61 ggtagagact gtggttgaac cggtgcaagc catgatgcgg actgaagccg ccgtcgagca
      121 ggtcgccgtc gtggaacaac agactccggc tgtccagcaa acgcccaccg cagcgccggt
      181 gatgcgccag gccgaacccg tgacgcaggt ggccaccgtc gtgagcgacg cccccgcggt
      241 cgaagcggca cccgcacccg tagaggctcc acaacaggtg gaacaggcga tcgttcagcg
      301 gccggtagaa accgtgcagc accggatcat tcaacagcga acggtgcaat cgcgaccggg
      361 cgaacgcccc gattacggct ggctgtcgca ggtcctgtgg tcttcggtgg atcaacgcaa
      421 gcgctacccg gccgaggcga aacggaacca ttgggaaggg cgcgtcatcc tgcgcctgac
      481 gctcgagcaa cggggagccg ccgtccacct gctcgatttg gtgctggaag aaagttccgg
      541 ccacaccatc ctggatcgac acaccctgga tatggtgcga aacgcctttc ccctgcaggt
      601 caaacaccag ctcgcgcaat caaaattgca ggtcgactta cccttctcct atcgtatgga
      661 ataatcacca cgtctgactg accaaccgga gggaatcatg ttgtcacact gtcattcgct
      721 acgatccacc gtcgccaccg tcgccaccac cgccgcactg atctgcggga ctgcactccc
      781 ggccgccgcg caacagaacg gcgacaaacc gtttcacgcc ctcgtaaccg atgcgaaaga
      841 catgcaaacc gacttgaaga atgtattgtt ctattgggaa gaaaaggtga gcgagacgtc
      901 cttcatcccc catgagttga agcacctgcc cgtgaaacgc ggcaccgcca cggtcaacat
      961 caagttcgac ggcgtcaaac aggtcgaggt gactcccgga gcggacaagg cgctgccaac
     1021 gctccacatc accttgacca acggcaagac gggcgacttt ccgctggcga tcgacggcag
     1081 cttcaaaggc gactcggatt tcggccaagt tgagctgccg gtccacggtc tcaagaagct
     1141 cgagtttaag taaacagcac cggggccccc tcccgatcat ccgtcgggag ccgcgtgagg
     1201 ccgaatgaac aggagccagg accagcagca ccgctcatga cgctccgtcg taccacctac
     1261 ctcgttatct tcacgctgct cgcactctcc gcgatgctcg ccgtgaaatg gaccgtgctg
     1321 actccggttc ctgcctcgga agtgacggga accgtgccgg cagggacggc cgtcgtgctc
     1381 gccccggtca ccaccgaggc gatccggcac acgatcaaag ccgtcggctc cttgcgggcc
     1441 aatgagagca tcatgatccg tccggagatc gcggggcgaa tccgtcaggt ctggtttcaa
     1501 gaaggtcagg cggtggagaa agaccagctg ctcatcgaaa tggacgattc ggaactccag
     1561 gcggaactgg ctcaagccgc ggcgcaactc aaagtcagcc gcctcaccta cgaacggctc
     1621 aaacgactcg acctcgacgg caagcggtac gtgacgaaac agcaactcga tgaagtggcc
     1681 ggcgcgctgc aagtctctca agccaaccac gcgctctatg tgaccagact ggccaagacg
     1741 aaaattcggg tccccttcac cggcgtcacc ggcatccggc gcgtctcccc cggcgacttc
     1801 gtcggttccg gccgggatct cgtgaacctg gaagatctcg acaccttgaa gatcgatttc
     1861 aaggtgccgg aaaccatgct gcgccatctg gcaccgggtc agtccctcga actgaccacg
     1921 gacgcctatc cggggaacac gttccatggc acggtctacg tgatcgatcc tcaggtcgac
     1981 ccgagcacga ggtccgtgca ggtgcgggcg agaattccga atccacagct gcggctcaga
     2041 cccggcatgt tcgcgcaagt gctcctcacg ttcggacagg aagaacgggc gctgctgatt
     2101 ccagaagagg ccgtcatgcc gaaacaggac aaggcctacg tctacctcgc ccaagacgga
     2161 accgcccaac agcgagaggt cacgctgggc acccgcacca ggggcttcgt gcaagtgctg
     2221 gacggactca cggaacagga tacggtgatc cgggtcggcc accacaagct gcagaacggc
     2281 gactccatac tggctctgtc ctcgacgcct cactaacgat aacccatcgg acgtccccat
     2341 gagactctcc gccctctcca ttgagcgacc cgtcctcgcc gccgtggcga cgctcctgct
     2401 gatgctcttc ggactgctct cctattcccg gttgccggtc cgcgagtatc ccgacaccac
     2461 cttccccatt gtctcggtca tgaccgtgta tccgagcgcc gacgcccaac tggtggagac
     2521 ggatatcacc tcggtgctgg aagatgcgct cagcggcgtc gaggggctcc gcacgttgcg
     2581 ttcgaccagt cgcgagggtt tatcgaccat cggcctcgaa ttctcgctga cgcgtaacct
     2641 ggatgcggcg gccaacgacg tgcgcgaccg cgtgacgaga gtgcaatacc tgcttccgca
     2701 gggcattgaa gcgccgttgg tcatgaagga ggacagcgaa gccgacggca tcatgt
//
LOCUS       DAORRQ010000176         2735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_877_length_2735_cov_4.114552, whole genome shotgun sequence.
ACCESSION   DAORRQ010000176 DAORRQ010000000
VERSION     DAORRQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_877_length_2735_cov_4.114552"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2735
                     /locus_tag="PK224_21295"
     CDS             <1..>2735
                     /locus_tag="PK224_21295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRB18340.1"
                     /translation="EILRACFALRNGRPSHIIVPQLILALDIEDLRTSVEPDREAQIL
                     RLAGEEACRPFDLANGPLIRGRLFRLDDMNHVLSLTLHHIVADGWSMDVLRRELASLY
                     EAFKDGRPSPLPALPIQYVDLIAAQRERPKGAADRRLLEYWANQLEGAPPLLNLPWDY
                     PRPAIQGHRGARHPWTLEPELARRLYEVGARYRATPFMLLVSALGLLVSRYSRQTDFC
                     IGTPVANRAGREAEQLVGCFVNTVVLRMDVSGNPSLPAVLDRVRVMVLGAQSHQDLSF
                     ERLVDELGVVRDLGHTPLFQVMFVLEDSPSDVRRFADLEASRLNVGTGTSVFDLTLEM
                     AQKTDGSLDAVFEYSTDLFAADTIARMAGHLQALLRQIVASPEASVSELSILSPGEQA
                     VELADSRSPREAYPQEASLHRLIEAQASKTPDAVALQCDRGPITYDSLNRLGNQLARY
                     LRVQGIVPDAPVALCVDRSLEMAIGLLGCLKAGATYVPVDPSYPAERQAAIFRDAQPH
                     LLLTQGRHLGGLPEHAAPVLALDAGWPAVAGLPDDNLVTGPNPDHLAYLLYTSGTTGN
                     LKGIGITHLELVKHGLAIVQDYDLYPSYRVLQFASVSFDVAVEECFPTWLAGATVVLR
                     PNEPVPAYGDFQGFLHAQGVTVVNLPTPYWAGWAEESERTAAAVPSSVRLVIVGSEKA
                     LPDDLRRWRRVAGERVGWCNSYGPTEATITASTYRLGPAQDDTTLRTIPIGRPIANVE
                     MYVLDRHLHPVPAGVPGDLYIGGPGLARGYHRQPALTAAQFIPHPFSHDRGARLYRTG
                     DVARRRSDGNLEFLGRADEQVKIRGFRVEPQEIECHVRAHPEVKDARVFLESDAPGDW
                     TAVLAGLGDEEQDRLVRDVEALSAAEARWLYDLESDGEQRRKTVIQRKPEFEVYL"
BASE COUNT          468 a          819 c          875 g          573 t
ORIGIN      
        1 acgagatcct tcgcgcatgt ttcgcgttgc gcaacggccg tccctcacac atcatcgtac
       61 cccaacttat cctcgctctg gatatcgaag acctgcgcac atcggttgag cctgacaggg
      121 aggcgcagat cctgcgcttg gccggagaag aagcctgtcg cccgttcgac cttgccaatg
      181 ggccgctcat tcgtggccgc ctcttccggc tcgacgatat gaatcacgtg ctctcgctga
      241 ccttgcatca catcgtggcg gacggttggt cgatggatgt gctcaggcgg gaattggcct
      301 ccttgtatga ggccttcaag gacggtcgcc cgtcacccct gccggctctc ccgattcaat
      361 atgtcgatct gatcgccgct cagcgagaga gacccaaggg cgcagctgat cgaaggttgc
      421 ttgaatattg ggcgaatcaa ttggaggggg cgcctccgtt gctgaacctg ccctgggact
      481 atccgcgacc ggccatccaa ggtcatcggg gcgcccgtca tccgtggacg ctggaaccgg
      541 agctcgcgcg tcgcctgtat gaagtggggg cccgctatcg cgccacgccg ttcatgttgt
      601 tggtctcggc cctcggtctc ctcgtgtctc ggtatagccg ccagaccgat ttctgcatcg
      661 gcaccccggt ggccaaccgc gcgggacgcg aagcggaaca actggtgggc tgttttgtca
      721 acaccgtcgt gctccggatg gatgtgtccg gcaatccctc gttgccggct gtgctggatc
      781 gcgttcgcgt gatggtgctc ggggcgcaga gccatcagga cctgtccttt gagcgtttgg
      841 tcgacgaact gggtgtggtg cgggatctcg gccacacgcc gctctttcag gtgatgttcg
      901 tgctggagga tagcccctcg gacgtccgtc ggttcgcgga ccttgaggct tcccggctga
      961 acgtcggcac ggggacctcc gtcttcgatc tcacgctgga gatggcacag aagaccgatg
     1021 gaagcctgga tgcggtcttc gaatacagta cggatctctt tgccgcggac acaatcgcgc
     1081 ggatggccgg gcatctgcaa gcactgctgc gacagatcgt cgcctcgccg gaggcatcgg
     1141 tgagtgagtt gtcgatcctc tcgcccggcg agcaggccgt ggagcttgcc gatagtcgca
     1201 gtccacggga ggcctatccg caggaagcct cgctccatcg cttgatagaa gcgcaggcga
     1261 gcaagacacc ggatgcggtt gcgctgcaat gcgaccgagg ccccatcacc tatgattcgt
     1321 tgaaccgcct gggcaaccaa ctcgcccgtt atctgcgagt gcaggggatc gtccctgacg
     1381 cgcctgtcgc gctttgcgtg gaccggtcgc tggagatggc catcggcctc ctgggctgtc
     1441 tcaaggccgg cgcgacctat gttccggtcg atccctccta tcctgctgaa cggcaggcgg
     1501 ccattttcag ggatgcgcag cctcacctct tgttgaccca gggaagacat ctcggaggcc
     1561 ttcctgagca cgcagcgcct gtcttggcgc ttgatgctgg atggccggct gtcgccggtt
     1621 tgcctgacga taatcttgtc accggcccga atccggacca ccttgcgtat ttgctctata
     1681 cgtccggcac cacagggaat ctcaagggca tcgggattac tcatctggaa ttggtgaaac
     1741 acggtcttgc gattgttcag gactatgacc tttacccgtc gtatcgcgtg ttgcagtttg
     1801 cctctgtgag ttttgacgtc gcagtagaag aatgtttccc cacctggctg gccggggcga
     1861 cagtcgtcct gcgccctaat gagccggttc cagcctacgg cgatttccag ggtttcttac
     1921 atgcccaggg ggtgacggtg gtgaacctgc cgactcccta ttgggccggc tgggcagagg
     1981 agagtgagcg cacggctgcg gccgttccat cgtcggtcag actggtcatc gtaggaagcg
     2041 aaaaggcgtt gccggacgac ctccggcggt ggcgacgggt ggctggagag cgggttggtt
     2101 ggtgtaattc ctatgggccg actgaagcca cgatcaccgc gagtacctac cggctaggac
     2161 cagcgcagga tgatacgacc ttgcggacca ttccgatcgg ccggccgatc gccaacgtgg
     2221 agatgtatgt cctggatcgc catctccacc cggtgccggc cggggttccc ggcgacctgt
     2281 acatcggcgg tccaggcctt gcgcgaggct atcatcggca accggcgctg acagccgcac
     2341 aatttattcc ccatcctttt agtcatgaca ggggggcgcg cctgtatcgc accggcgatg
     2401 tggcccgccg ccgttcggac ggaaatcttg aatttctcgg gcgtgccgat gagcaggtca
     2461 agattcgtgg gttccgagta gaaccgcaag agatcgaatg ccacgtgcgc gcccatcccg
     2521 aggtcaaaga cgcgcgcgta tttctggaat ccgacgctcc cggcgattgg accgcggtcc
     2581 tggccggtct cggtgacgag gagcaagacc ggctggtgcg ggacgtggaa gccctgtcgg
     2641 ctgcggaagc gcgctggttg tacgacctgg agtcggatgg agaacaacgg aggaagacgg
     2701 tgattcaacg gaagccggag ttcgaggtgt atctc
//
LOCUS       DAORRQ010000177         2697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_886_length_2697_cov_109.793717, whole genome shotgun sequence.
ACCESSION   DAORRQ010000177 DAORRQ010000000
VERSION     DAORRQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_886_length_2697_cov_109.793717"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PK224_21300"
     CDS             complement(<1..520)
                     /locus_tag="PK224_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18341.1"
                     /translation="MRLHDTTVNQHPPRLVGRRAKGLLTALMLTLWFCDFGTGGSALA
                     QNIQFTPGALSLTVAKGGTVSGTLSLKKSDTAQHTYFISANQSWIWLNPPYGSTQTIS
                     TETDVLTVTVNTAAMGLAAGTYSGTIYIGQAGPGVSTTWRIPVTTTVTATGTTPPPPT
                     GSLTPMLQAFPSA"
     gene            1029..2060
                     /locus_tag="PK224_21305"
     CDS             1029..2060
                     /locus_tag="PK224_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18342.1"
                     /translation="MFSLRRLMCAVCACLAGFGALPAAAEVAPAQSSSIARVDIGLSE
                     WFSQGETVWSHNASGLDANLGNPSSKLKYKDTGTNVTEISGRVQLKNKIFFRGAFGYG
                     SIGGGRLTDDDFLSAQGAAAQGATVSGEHRFSRTFSDIGGDNLWYLNGDIGATTFTFP
                     ENKGTLGMFVGLQYWRERHVANGVTQAECTTASSPNQDFRCSPAGTVGFRNQTVITNT
                     STWVSGRFGGEVEYRVDKRISIEAKIAMLLSYLNNEDVHHLRTDLAQDPSFRMTGLGL
                     GTNSDINLRIRIWDRLYLTGGYRVFWNRVLVGDQWKLYGSDGSSTTASLNQFQTLRHG
                     PTIGLTYSF"
     gene            complement(2251..>2697)
                     /locus_tag="PK224_21310"
     CDS             complement(2251..>2697)
                     /locus_tag="PK224_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18343.1"
                     /translation="PLLIMTTEILRNLLYDAAGGEIDVRLDTLGLVIMDESQYLADPE
                     RGVVWEETLIFCPSQARLLLLSASIGNPQDIADWLTAIRPNPCALIRHTKRSVPLRAG
                     YMHPNEKLTPLFRTAGIPYGQPHLLHPEAKRLFTEYEEETGPSRSR"
BASE COUNT          541 a          727 c          847 g          582 t
ORIGIN      
        1 gtgcggacgg gaaggcctgg agcatgggcg tcaatgagcc ggtcggcggc ggcggtgtcg
       61 tgccggttgc cgtcacggtg gtggtcaccg ggatccgcca ggtcgtagac acgccgggtc
      121 cggcctgtcc gatataaatc gtgcccgaat aggttccggc cgcaagtccc atggcggcag
      181 tattcaccgt gactgtgagg acgtccgttt ctgtgctgat ggtttgggta ctgccgtagg
      241 ggggattcag ccagatccag gattggttgg cgctgatgaa gtaggtgtgt tgcgccgtat
      301 ccgatttttt caggctgagg gtgccgctga ccgttcctcc cttggcgacg gtcaaggaca
      361 gtgcgcccgg tgtaaattgg atgttctgcg cgagtgccga tcctcccgtg ccgaagtcgc
      421 agaaccacag agtcagcatc aaggccgtaa gcaatccctt cgcgcgtcgt ccgacgagcc
      481 ggggtggatg ctggttcact gtggtgtcgt gcaacctcat agtcagtact cccttaggtg
      541 atattcccga aaagaaaaaa ggaaacggtc gttggtgagc aacagtcgtg ccacaacaaa
      601 tcgcttcggc gttcttctga attatttgtc gggaatcgct cagcggagtt cggtgagaat
      661 cccgtcagga ttactatcag tgccgaccct aacggaagag gacggaatag gcaagtaaaa
      721 atttcacttg cttcccgtag gactcgtggg aagacatctg gtagaaaaaa gtggatgagg
      781 ccgatgacca ctgtagggtg tcggcacctg tctgtacaaa accgatagcc ttgccgttca
      841 cgatgtagag agattcccta ggtatgcacc agagacgttt gcgggtggcc atacgggcct
      901 atgggcatga ggtgtgagaa agttgttgac catgttcggt aggtagggta tctgtgggcg
      961 cgccgtttcc gaggggttac gtagtccgtt gatatcgatg cggagccggt ggcagagggg
     1021 gccacgacat gttctcgcta cgacgtctca tgtgtgcggt gtgcgcgtgc ctggccggtt
     1081 tcggcgcgct tcctgctgcg gcggaggttg ctcctgccca gtcctcctct atcgcccgtg
     1141 tcgacatcgg cctgtccgaa tggttcagcc agggtgagac ggtctggagc cacaacgcct
     1201 caggcctgga tgccaacctt ggaaatccaa gttccaaact caagtataag gataccggca
     1261 ccaatgtgac cgaaatcagc ggtcgagtgc agttgaagaa caaaatcttt ttccgcggcg
     1321 ccttcgggta cggctcgatc ggcggaggcc ggctgacaga tgacgacttt ctcagcgcac
     1381 aaggggctgc ggctcagggc gcgaccgtca gcggagaaca tcggttctcg cgcaccttca
     1441 gtgacatcgg gggagacaat ctctggtatc tgaacgggga tatcggtgcg acgacgttca
     1501 cctttcccga aaacaaaggc accctcggga tgtttgtggg gttgcagtac tggcgggagc
     1561 ggcatgtggc aaacggcgtc acacaagccg aatgcaccac ggcatcctct ccgaaccagg
     1621 attttcgttg ttcgcctgcc ggtacggtcg ggttccgtaa ccagacggtg attaccaaca
     1681 cgtctacctg ggtatcaggc cggttcggcg gtgaggtcga atatcgggtc gataagcgca
     1741 tcagcattga ggccaaaata gcgatgctcc tgtcctattt gaacaatgaa gatgtgcatc
     1801 acctcagaac cgacctcgca caggatccga gcttcagaat gacgggcctg ggcctgggta
     1861 ccaattcgga tatcaatctg cgaatcagga tctgggaccg gctctacctg acgggcgggt
     1921 atcgcgtgtt ctggaaccgc gtgctcgtcg gtgatcagtg gaagctgtac ggctccgacg
     1981 gctcgtcgac taccgcctcc ctgaaccagt ttcaaactct ccggcacggg cccaccatcg
     2041 ggctgaccta ctctttctaa tcgtcgctcg tctctcgatg cagaacggga gcaaatagcg
     2101 tattgcttat ggcgtatagt cgggaacggc agcaacccgc ggcgttctgc cttcatccat
     2161 gagccatacg ctcttctttc ccgccatcag cgcgaatgcc tgccgagaga cgtttcacgg
     2221 ttcccgagtg acggtattcg gcggcagcgc tcaccgcgat cgagatgggc cggtttcttc
     2281 ttcgtattcc gtgaagagcc gtttggcttc aggatggagg aggtgcggtt gaccatacgg
     2341 gattccggcg gtccggaaga ggggcgtcag tttctcgttc gggtgcatgt atccggcccg
     2401 gagcgggacc gatcgcttgg tatggcgaat gagcgcacag ggattcgggc ggatggccgt
     2461 cagccaatcc gcaatatcct gcgggttccc gatcgaggcg gacaacaaga gcaggcgggc
     2521 ctgcgacgga cagaagatca aggtctcctc ccataccacc ccgcgttccg gatcggcgag
     2581 gtattgggat tcgtccatga tcaccagccc caacgtatcc aggcgcacgt cgatttcgcc
     2641 ccccgccgca tcgtagagca gattgcgcaa aatttcggtg gtcatgatga gcagggg
//
LOCUS       DAORRQ010000178         2659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_894_length_2659_cov_8.408986, whole genome shotgun sequence.
ACCESSION   DAORRQ010000178 DAORRQ010000000
VERSION     DAORRQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_894_length_2659_cov_8.408986"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2659)
                     /locus_tag="PK224_21315"
     CDS             complement(<1..>2659)
                     /locus_tag="PK224_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRB18344.1"
                     /translation="EDAKEGHVERTAMMERQHQRIMEKINEQMVHDAEVQRTGGQYNN
                     INMMHQYGAGNQDLLLMSNPSAEPVSMGGGRCPANAPVRTYDVSAINVEITLNMWLDF
                     YPGYMYVLTENIDKVRAEETKNKDARDVEGYNPGAVMNGLQNQWIQPLVLRGNQGDCV
                     KLTLRNQLEGTEEVSLNIHGSSMVMAATGQPASTTNPDSIAGPGKAVDMEWYIPPTQQ
                     EGSRQFHSYSNDRELTVMGLFGSFVIEPKGSEYLEPLGTGDPQPTKSGWQAIIKNGAG
                     PDFREFVLFYHEVGDEAFRPLNKKGDFIPQRDPLTDVYRPVARALNYRSEPFGVDNMQ
                     TQHEYFGFEDESMAYSAYTFGDPATTIPRSYLGDPAKFRLVHGGSEVFHSHHPHGGSI
                     RWPRSPRAIDEMPLWHTAKNGPVKYPVIRTKSDRVDVEVIGPSETMDLETECGSGLCQ
                     QLAGDFLFHCHVAHHYIAGMWGYWRVYNTIQQGANHNDVMPDLLELPDRKGRIKVGVT
                     SDELIGKTVDWFGKQFTIIEKGKTDWKASPALVTVKDWVEMQFPPQGLPGHKKDEREQ
                     TKSYDASVLNWGWQGNRAMTEPENTVPNPRYQSAHPNERLPIVFEPLTGKVSWPHLKP
                     HFGRRVPFSKGHNPSPWLEMIHLEEDGLPSSYPAKPGENGRWSLCPENAGSKKYNVHF
                     IHTPMMLADKQGNTPAIMDEGGLIYVLHEEEAAIRKNNDLKFPLVVRSNIYDCVDWML
                     TSEWDDDDFSNFHASKVNTHWHFLQFDNQSSDGVITGFSNEQSVRPFTMLEKKTDKGL
                     PLPLNTTFTKAAKKGERVITVKNAAQYHPEIEILIGADNVGGNEVGRIKSIKGNEITL
                     VRPLKNDHPVKDIVTVEFVRQRFWVDSDVG"
BASE COUNT          490 a          837 c          729 g          603 t
ORIGIN      
        1 gcccacatcg gaatcgaccc agaaccgctg ccggacgaac tcgaccgtca cgatatcctt
       61 caccggatga tcgttcttca agggacgaac cagagtgatc tcgttcccct tgatggactt
      121 gatccggccg acttcgtttc cgccgacatt gtccgcgccg atcaggattt cgatctccgg
      181 atgatactgc gcggcgttct tgacggtaat gactcgctca cccttcttcg ccgccttcgt
      241 gaaggtggtg ttcaagggca acggcaaccc cttgtcggtc ttcttctcca gcatcgtaaa
      301 cggacgcacc gactgttcgt tcgagaagcc ggtgatcacg ccgtccgacg actggttgtc
      361 gaactgcagg aaatgccaat gggtattgac cttcgaggca tgaaaattcg agaagtcatc
      421 gtcgtcccat tcgctggtca acatccaatc gacgcaatcg tagatgttcg accgcacgac
      481 caacggaaac ttcaggtcgt tgttcttccg aatggccgct tcttcttcat gcaagacata
      541 gatcaacccg ccttcatcca tgatcgccgg cgtgttgccc tgcttgtcgg ccaacatcat
      601 cggcgtgtga atgaagtgca cgttgtattt cttcgaaccg gcattttccg ggcacaggct
      661 ccatcgcccg ttctctcccg gcttggccgg gtaggaactc ggcagcccgt cctcttccag
      721 atggatcatc tccagccacg gggaaggatt gtggcctttg gagaacggca cccggcgacc
      781 gaaatgtggc ttcaagtgag gccacgacac cttgccggtc aacggttcga acacgatcgg
      841 cagccgctcg ttcggatgcg ccgactgata ccgcggattc ggcaccgtgt tttcaggctc
      901 ggtcatcgcg cgattgccct gccagcccca gttcagcacg ctggcatcgt aggacttggt
      961 ctgctcgcgc tcatcttttt tgtgaccagg caagccctgc ggcgggaact gcatctcgac
     1021 ccagtctttc acggtcacca gagccgggct cgccttccaa tcggtcttgc ccttctcgat
     1081 gatcgtgaat tgcttgccga accaatcgac ggtcttgccg atcaactcgt cggacgtgac
     1141 acccaccttg atccggccct tccggtccgg caactccaac aggtccggca tcacatcgtt
     1201 gtggttcgcg ccctgctgaa tggtgttgta cacgcgccag tagccccaca tcccggcgat
     1261 gtagtgatga gccacgtggc aatggaacag aaagtctccg gccaactgct gacagagtcc
     1321 ggatccacac tccgtttcga ggtccatggt ctccgacggg ccgatcactt ccacgtccac
     1381 acgatcggac ttcgtccgga tcaccggata cttcaccgga ccgttcttcg ccgtatgcca
     1441 gagcggcatt tcgtcgatcg cccgcgggct gcgcggccaa cggattgaac ctccgtgcgg
     1501 atgatgtgaa tggaacactt ccgatccacc gtgcaccagc cggaacttgg ccgggtcacc
     1561 gagatagctg cgcggaatgg tcgtggccgg atcgccgaac gtgtaggcgc tgtaggccat
     1621 cgactcatct tcgaagccga aatattcatg ctgggtctgc atgttgtcca cgccgaacgg
     1681 ctcgctgcgg tagttcaatg cgcgagcgac cgggcgatag acgtcggtca acggatcccg
     1741 ctgcgggatg aagtcaccct tcttgttcaa tgggcggaag gcttcatcgc cgacctcgtg
     1801 atagaacagc acgaactcac gaaagtccgg tccggctccg ttcttgatga tcgcctgcca
     1861 gccgctcttg gtgggctgag gatctccggt tcccaacggc tcaagatact ccgagccctt
     1921 cggttcgatc acgaacgacc cgaagaggcc catcacggtc aattcgcggt cgttgctgta
     1981 cgaatggaac tggcggctac cctcctgctg tgtgggcgga atgtaccatt ccatgtccac
     2041 ggcctttccc ggtccggcaa tgctgtccgg attggtggtg ctggccggct ggcccgtcgc
     2101 cgccatgacc atgctggagc cgtgaatgtt gagactgacc tcttccgtgc cctcgagttg
     2161 attgcgaagc gtgagcttga cgcaatcgcc ctggttgccg cgaaggacga ggggctgaat
     2221 ccattgattc tgcaacccgt tcatcactgc gcccgggttg tacccttcca cgtcgcgcgc
     2281 atctttattc ttcgtttctt ccgcgcgcac cttgtcgata ttctccgtca gcacgtacat
     2341 atagccggga taaaaatcga gccacatatt cagcgtgatc tcgacgttga tcgcggagac
     2401 atcgtacgta cgcaccggtg cattggccgg gcagcgcccg ccgcccatcg acacaggctc
     2461 ggcggaggga ttggacatca gaaggagatc ctgattaccc gcgccgtact ggtgcatcat
     2521 gttgatgttg ttgtactgcc cgccggtccg ctgcacctcg gcgtcatgca ccatctgctc
     2581 attgatcttc tccatgatgc gctggtgctg ccgctccatc atggccgtcc gctcgacatg
     2641 cccttccttg gcatcctcg
//
LOCUS       DAORRQ010000179         2656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_896_length_2656_cov_6.176855, whole genome shotgun sequence.
ACCESSION   DAORRQ010000179 DAORRQ010000000
VERSION     DAORRQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_2656_cov_6.176855"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="PK224_21320"
     CDS             <1..2070
                     /locus_tag="PK224_21320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HRB18345.1"
                     /translation="PPARDRPRTEKPVKVPEILVKDVQEREGETSYVAEDTKTATKID
                     IPLIETPQSISVITRARMQAQEVNSIGEALRYTPGVRGEPFGFDSRLTWLQFRGFDNT
                     TNGLFKDGLQLRNPAFGISYNLEPYGAEQLDVLRGPASFLYGQGSPGGLLNYVSKRPT
                     LQSLHEVQFLAGSFGRYEGRFDFGGRINDSDTFSFRMTGLIRESGTQIDNVPNDRVYI
                     APALTWRMASTTSLTFFATYQKDRAGAFQWLPADGTLGFNPNGFVQPHRFTGQPSVDR
                     YNRTEHSVGYEFHHQLSENWKFLQKMRYNSNETDNVSTYVLGLEPDRRTAIRDQWSSY
                     GKLTAIALDNQISGRLSTGPMQHTLLAGLDFQRISVRHNQNFAEASSIDIFNRYDYGT
                     LGVVPYFDSKQDTKQLQTGVYIQDQIKMFDKWLLTLGGRHDWARNEATDNVAGTTSTQ
                     SDRKATGRAALTYLFDNGLAPYFSYSTFFLPSIGIGADGKQFKPESGQQYEAGIKYQI
                     PRTRAFVTAAVFDLTRENYVQTDPGTFLPVQRGKARSRGLELEGVASFDSGWDLIAAY
                     TLLDNKVQETVDPSEQGKRLVQVPAQFSSLWAKYTVPDGKWKGFGIGGGARYTGYNYA
                     DTPNAFRVPSYVVGDAVIDHTWNHYRFALNINNILNHNTFGCFNEGLTCNFGERRTVV
                     GSVAYRW"
     gene            2284..>2656
                     /locus_tag="PK224_21325"
     CDS             2284..>2656
                     /locus_tag="PK224_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRB18346.1"
                     /translation="MKGYIVQIEDATRDNQHYRRVLFTAKHSQLVLMNLKPGEEIGEE
                     VHGMDQFIRFEEGEGTVVLDGEKHAVSDGFAVVIPAGTRHNVVNSGKTAALKLYSLYS
                     PPEHKDGIIHKTKQEADADHDH"
BASE COUNT          639 a          796 c          726 g          495 t
ORIGIN      
        1 ccgccggcga gggatcgccc tcgcacagag aaaccggtca aagtgcccga gatcctggtg
       61 aaggacgtac aggagcgcga gggtgagacg tcctatgtgg cggaggacac gaaaaccgcc
      121 accaaaatcg acatcccgct gatcgaaacg ccccagtcca tcagcgtcat cacgcgcgcc
      181 cgcatgcagg cgcaagaggt gaactcgatc ggcgaagcct tgcgatacac accgggcgta
      241 cgaggcgaac cgttcgggtt cgattcccgc ctgacctggc tccagtttcg cgggttcgac
      301 aacaccacga atgggctttt caaggacggt ctgcaactcc ggaatccggc cttcggcatc
      361 agttataacc tggaacccta cggcgcggaa cagttggatg tgctccgtgg accggcgtcc
      421 tttctatatg gacaagggag tcccggcggc ctcctcaact atgtgtccaa acggccgaca
      481 ctgcaatcgc tccacgaggt gcagtttctg gccggcagct tcggccggta cgaaggccgg
      541 ttcgatttcg gaggccggat caacgacagc gacaccttct ccttccgcat gaccggcctg
      601 atccgggaaa gcgggacaca aatcgacaat gtgccgaacg atcgcgtgta catcgcaccg
      661 gccttgacct ggcgaatggc ttccactacc agcctgacct tcttcgccac ctaccagaaa
      721 gatcgcgcag gggctttcca atggttaccg gccgatggaa ccctgggctt caaccccaat
      781 ggattcgttc agccacatcg gtttaccggt caaccttcgg tcgatcgata caaccgcacc
      841 gaacattccg tcggctacga attccaccat caattgtctg agaattggaa gttcctgcag
      901 aaaatgcgct acaactcgaa cgaaacggat aacgtctcta cctacgtcct tggactggaa
      961 ccggaccggc ggacggcgat ccgagaccaa tggagcagct atggcaagct gaccgccatt
     1021 gcgctcgaca atcaaatcag tggaagactc tcgaccggcc cgatgcaaca taccctcctg
     1081 gccggcctcg attttcaacg gatctccgtg cgccacaatc agaacttcgc ggaggcctca
     1141 tccatcgaca tcttcaatcg atacgactac ggcacgctcg gagtcgttcc ctatttcgac
     1201 agcaagcagg atacgaagca attacaaacc ggcgtctaca tccaggacca gatcaaaatg
     1261 ttcgacaagt ggttacttac gctcggaggc cgccacgatt gggcgcggaa tgaagcgacc
     1321 gacaacgtcg ccggcaccac cagcacccag agcgaccgca aggcaaccgg gcgcgccgcc
     1381 ttgacctacc tcttcgacaa cggcttggcg ccctatttca gctattcgac ttttttccta
     1441 ccctcgattg gaatcggcgc cgacgggaag caattcaaac cggaaagcgg ccagcagtat
     1501 gaggccggaa tcaagtacca gatcccacgc actcgcgcct ttgtgacggc ggcggtattc
     1561 gatctcacgc gggaaaacta tgtgcagacc gaccctggca cattcttgcc ggtacaacgc
     1621 ggcaaagctc gctcccgcgg cttggaactg gaaggtgtag ccagtttcga ttccggttgg
     1681 gacctgattg ccgcctacac cctgctggat aacaaagtgc aggaaacggt cgatccgagt
     1741 gaacaaggca aacgtctggt tcaggtaccg gcacagttca gttccttatg ggctaaatac
     1801 accgtgcccg acgggaagtg gaaaggattc ggaatcggcg gcggggcgag atataccggc
     1861 tacaactatg ccgatacgcc caatgcgttc cgggttccca gttatgtggt cggcgacgcc
     1921 gtcatcgacc acacttggaa ccattaccgg tttgcgctga acattaacaa catcctgaac
     1981 cacaatacgt tcgggtgttt caacgaaggc ctgacctgta acttcggcga acggcgcacc
     2041 gtggtgggga gcgtggcgta tcgatggtga gaagcatcag ccggaaccgg catattgact
     2101 ccccttcagg attcagcaaa caggagtgcc gttcctgcga ctcgtcggga cgtattggcc
     2161 gctcacgggc atcgtgtttg agatgaactc atcatgatcc tgccgactac ccacctcatt
     2221 cagcgcagcc gacgggtcaa ccgcgcactt gtctgaagca attacgcacg gaggacgttc
     2281 cccatgaaag gctacatcgt tcagatcgaa gacgccacgc gcgacaatca gcattaccgc
     2341 cgggtactct ttacggcgaa acactctcaa ctggtcctaa tgaacctcaa gcccggtgaa
     2401 gaaatcggag aagaagttca cggcatggac cagtttatcc gattcgagga aggggaggga
     2461 accgtggtcc tggacggaga gaaacacgcg gtgagcgacg gctttgcggt cgtcatcccg
     2521 gccgggacca ggcacaatgt cgtcaatagc gggaaaacgg cagcgcttaa gctgtacagt
     2581 ctctatagcc ctccggaaca taaagatgga atcattcaca aaacgaagca ggaagccgac
     2641 gcggatcacg atcatc
//
LOCUS       DAORRQ010000180         2626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_905_length_2626_cov_4.739790, whole genome shotgun sequence.
ACCESSION   DAORRQ010000180 DAORRQ010000000
VERSION     DAORRQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_2626_cov_4.739790"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1002
                     /locus_tag="PK224_21330"
     CDS             97..1002
                     /locus_tag="PK224_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18347.1"
                     /translation="MIVQTLVRSTVMAVGMCGFLGIPSGPGQARATEAGQTIAQSADY
                     FPDTVGTRWQYRGQISEGPLQTIENKYFFNVSSVTGTRTLKGGVVVNVFHDTNPGNHG
                     ASDSFYRRDAAGVVYYGSEPGTPLEKQLVPYQIIRFPMKVAQSFQQFNRMDIDFGSDI
                     DGDGTNEHVDVEGVSTVIGQESITVPAGTYPDAVRVEARMTLKIHLSSADRTAVGTDV
                     MTAWFAKGVGLVKYIERQELAALKEDRGNTTDITEELEEVTVKSAPALQGRSESSPER
                     LLADDTRDHELFQVLFAPGLRPYTR"
     gene            complement(892..>2626)
                     /locus_tag="PK224_21335"
     CDS             complement(892..>2626)
                     /locus_tag="PK224_21335"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRB18348.1"
                     /translation="AYLVRRLLENTSNESFLRKEYVESQPLSLLLSPPDATLPSPPSP
                     MAPEAGASSTVGSAHFVNEPAADFSRDDVRVAMAEAIDQVRKQLGQRLEVSRLNTHLP
                     TGPDLRSFNPGQPEQLVGIVQSYLPADVAGLLKVAESHAEEWKHRAVAERVAVMRKAA
                     SLMREQRWELAAWEVFEEAKPWREADADVAEAVDFLEYYAGEMEQLDPPPRLGRYPGE
                     LNELFWSPRGVTVVIAPWNFPLAIPAGMVAAALVSGNPVLFKPSERASIMGYRLATIL
                     RQAGVPAGVLQYVPGGPDVGRELVTQASVATIAFTGSKDVGLGMVQAAAVQQPGQKFV
                     KRVIAEMGGKNAIIVDDTADLDEAVTGVVQSCTGYAGQKCSACSRVILLDSIHDAFIQ
                     RLADAVMSLRIGAAEDPATQVGPVIDGRAQKRIRDYLEIGRREGRVVVDRSMEGPGHA
                     VGPVVIAGIEPTHRLAQEEIFGPVLAVMRASSFEQALQLANGTAYALTGGLYSRSPRH
                     IELARQAFDVGNLYINRSITGALVGRQPFGGHRLSGIGAKAGGEEYLKQFMVARVVSE
                     QTLRRGFAPTL"
BASE COUNT          553 a          852 c          767 g          454 t
ORIGIN      
        1 tatgagagga ggcacaggag aaggcaaggc tggacctgcc ggcttgctgc ggcgaatcgg
       61 gtatggtacc gtgatttttt agactggagg acagccgtga tcgttcagac cttggtgcgc
      121 tcgaccgtga tggcagtggg catgtgcggg ttcctgggca tcccgtcggg tcctgggcag
      181 gcccgggcaa cggaggcagg acaaaccatc gcccagtctg ccgactattt ccccgacacc
      241 gtcggcacac gctggcagta ccgcggacag atctcggaag ggcccctcca aacgatcgag
      301 aacaaatact tcttcaacgt gtcgtcggtg accggaaccc gcactctcaa gggcggcgtg
      361 gtcgtcaacg tgtttcatga caccaaccca ggcaaccatg gcgcatccga cagcttttac
      421 cgccgcgatg cggccggcgt ggtctactac ggctccgagc cgggcacgcc gttagagaag
      481 cagctcgtgc cgtatcagat catccggttc ccgatgaaag tggcccaatc cttccaacag
      541 ttcaatcgca tggacatcga tttcggcagt gacatcgatg gggacggcac caacgaacat
      601 gtggacgtcg aaggagtttc gacggtgatc ggccaggaat cgatcacggt gcccgccggc
      661 acgtatcccg acgccgtgcg cgtagaagcg cggatgaccc tcaagattca tctctcctcc
      721 gccgatcgga cggcggtcgg caccgatgtg atgacggcct ggttcgccaa gggagtgggg
      781 ctggtgaaat atatcgaacg gcaggaattg gccgcgctca aggaagaccg gggcaacacc
      841 accgatatca cggaagaact ggaagaggtc accgtaaaat ccgcgccggc actacagggt
      901 cggagcgaat cctcgccgga gcgtctgctc gctgacgaca cgcgcgacca tgaattgttt
      961 caggtactct tcgcccccgg ccttcgcccc tatacccgat agccggtgac cgccgaacgg
     1021 ttgccgaccg accagggccc cggtaatcga acggttgatg tagaggttgc cgacatcgaa
     1081 cgcctgacgc gccagctcga tatgccgggg actgcgcgag tagagtcccc cggtcaatgc
     1141 gtaggcggtg ccgttggcga gctggagcgc ctgttcgaac gatgaagccc gcatcacggc
     1201 cagcacgggg ccgaaaattt cttcctgtgc cagccggtga gtcggctcga tccctgcgat
     1261 gactaccggt cccacggcat gaccggggcc ctccatcgac cgatcgacca ccacccggcc
     1321 ttcgcgtcgc ccgatctcca gatagtcccg aatccgtttc tgagcccgtc cgtcgatcac
     1381 cggaccaacc tgggtagccg gatcctcagc cgcgccgatg cgcagactca tcaccgcatc
     1441 ggccagtcgt tggatgaacg catcgtgaat cgaatctaag aggatcacac gtgaacaggc
     1501 ggaacatttc tgtcccgcat aaccggtaca cgattgcacc acaccggtca cggcttcatc
     1561 gagatccgcc gtgtcatcga cgatgatggc atttttgccg cccatctccg cgatgacgcg
     1621 tttgacgaac ttctgtcccg gttgttgaac cgcagcggcc tgcaccatgc ccaatcccac
     1681 atccttcgat ccggtaaagg caatcgtcgc gacagacgcc tgagtcacca gttcacgccc
     1741 cacgtcggga ccacccggca cgtactgcag gacgcccgcc gggaccccgg cctgcctcag
     1801 aatcgtggcg agccgatagc ccatgatcga cgcgcgctca gacggtttga acaagaccgg
     1861 atttccggag accagagccg ccgcaaccat accggcagga atggcgagcg gaaaattcca
     1921 gggcgcgatg acaaccgtga ctccgcgcgg actccagaac aactcgttca actcaccggg
     1981 ataccggccc aagcggggcg gcggatcaag ctgttccatt tctccggcgt agtactccag
     2041 aaaatcgacc gcctcggcca catcggcatc ggcctctcgc cagggcttgg cctcctcgaa
     2101 cacttcccag gccgccaact cccaacgttg ctcgcgcatc aacgaagcgg ccttccgcat
     2161 cacggcgacc cgttccgcaa ccgcacgatg tttccattcc tcggcatgag actcagcgac
     2221 cttcaagaga ccagccacgt cggcaggcag ataactctgc acgatgccga ccagttgttc
     2281 cggctgaccg gggttgaagg aacgtagatc cggccccgtc gggagatgcg tattcagcct
     2341 cgagacctca agccgctgcc ccaattgttt gcgcacctga tcgatggcct cggccatggc
     2401 gacccgaacg tcgtctcgtg aaaaatccgc cgccggttcg ttcacaaagt gagcgctccc
     2461 cacggtggaa gaagcgccgg cctcaggcgc catcggggaa ggaggcgagg ggagggtcgc
     2521 atcgggcgga gacagcaaca aagacagagg ctgcgattcc acatattcct tccgcaagaa
     2581 cgattcgttc gacgtgttct ccaacaaccg ccgcaccaga taggcc
//
LOCUS       DAORRQ010000181         2611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_908_length_2611_cov_248.857199, whole genome shotgun sequence.
ACCESSION   DAORRQ010000181 DAORRQ010000000
VERSION     DAORRQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_908_length_2611_cov_248.857199"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PK224_21340"
     CDS             complement(<1..793)
                     /locus_tag="PK224_21340"
                     /inference="COORDINATES: protein motif:HMM:NF037161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HRB18349.1"
                     /translation="MSKATVEQPKASRGRKRPASSAPWVAIDVGYGYTKILTQEGQAH
                     VFPSLVAPAELSNIDLSLSDPQETVKLEGAEYIVGQAAVSRGFRFSEEYDGWWMTTRY
                     KALLHYAAANFVPPGSRIVTGIPLHIFGSQRAQHQISDVFRKALKAPAVAVLPQGFGA
                     LCLAISTNSALAQGRVALVDIGSRTTELICFSDGQYLNHESAGVVLGVGQVYTQVAQK
                     LSSQHQRQIDAYEVDWALREDKPIKIKGGVVERQALEALVQHYVRP"
     gene            1339..1752
                     /locus_tag="PK224_21345"
     CDS             1339..1752
                     /locus_tag="PK224_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18350.1"
                     /translation="MPRQRGGALHEEYMNRFYELLHEARVRAPRLVGLWLNILLDEDS
                     PRIKRRFRGLDRYIEQMILKYPAYSARALNNLVRKQRQMGLNAEHVVRARIRMVKAKL
                     RSRAYRQAKRASLAGDVQWIGREEDARHLQNTGDA"
     gene            1749..2261
                     /locus_tag="PK224_21350"
     CDS             1749..2261
                     /locus_tag="PK224_21350"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRB18351.1"
                     /translation="MMGRTHCLVPAALAAWLFGPDPTILLISAGTGLLPDIDEPQSTI
                     GYRLFLLAKGFKGLVGHRTYSHSLVGIVTAAALAVIFDLPWIWIEAGLIGWVSHLVLD
                     ALSGGVPLWWPLYATNRERWVLARWKPFGFADHLILVLAGVALGAAAIHGNWLGWWIE
                     LLKRDISVSQ"
BASE COUNT          538 a          704 c          808 g          561 t
ORIGIN      
        1 acggtcgcac ataatgctga accagcgcct ctaaggcttg ccgctcgacc acaccccctt
       61 taatcttgat cggcttgtcc tcgcgcaacg cccagtccac ctcgtaggca tcgatctgtc
      121 gttgatgttg agaagacaac ttctgggcca cttgtgtata cacctgtcca acccccaaca
      181 cgacccccgc cgattcgtgg ttcaagtact gtccatcgga gaaacaaatg agttccgttg
      241 tgcggctgcc gatatcgact aaggccaccc gtccttgcgc gagtgcggag ttggtgctga
      301 tcgcgaggca caacgcgccg aatccttgag gtaagacggc cacggcggga gctttgagcg
      361 ccttccggaa gacgtcgcta atttggtgct gggccctctg agagccgaag atatgtaggg
      421 gaatgcctgt cacgatgcgg gagcctggtg gaacgaagtt ggcggccgca tagtgcagca
      481 gcgctttgta gcgcgtggtc atccaccagc cgtcatattc ctcgctaaag cgaaaccccc
      541 gggacactgc cgcttggccc acaatatact ctgcaccctc gagtttcacg gtctcctggg
      601 gatcggacaa gctcaggtcg atattggaga gctccgccgg cgccaccaga gacgggaaga
      661 cgtgcgcctg tccctcctga gtcaggatct tcgtgtatcc atacccgacg tcgatcgcga
      721 cccatggagc cgaggacgcg gggcgtttgc gtccacgaga cgctttcggt tgttcaacag
      781 tcgccttact catacacagc ctccgttcgg catgcggttc agattggaac cactcagtta
      841 gggatgtatt actaacttcg tggcgctaga acgggccagt acagcactaa atccatttgt
      901 catacatgtc agacaaataa gtaagacatt tcgctgtcgt gcgtacgcag accaaaatga
      961 gaagcccgtg aatgcacaac gagttcatgg gatcggcctg acatccacgg ttgtgtgggg
     1021 gaggcagccc cccaggatag gagtaatgtc atacacctgt ccggcggatg gctcgcgagt
     1081 gcgccagggc atggatttag cgcaagctgt ccggcagcgc ggcccacgtc ttttcaacac
     1141 tccatcggcg gtttcccgtc accatcatgc tggccgatag cgacgtgcgc actgtgagta
     1201 gggtgcggca gcactgtcgc gtacgcattt cgtataccgg atgcatatat ggtgtcttat
     1261 attgagactt gtcatcgtcc tcccactcgc cccctgacgg cgttccgtat ccggtaatac
     1321 ctcccccagg aggtgcctat gccacggcag cgagggggag cgttacacga agagtacatg
     1381 aatcgctttt acgagctatt gcacgaggcg agagtccgag caccacgatt ggtgggtctc
     1441 tggctcaaca tcctcttgga tgaagattcg cccaggatca agcgacggtt tcgagggctc
     1501 gaccggtaca ttgaacagat gattctcaag tatccggcgt actcggcgcg ggcgttgaat
     1561 aatctggtga ggaagcagcg ccagatggga ctcaatgcag agcatgtggt tcgggcgcgg
     1621 attcggatgg tcaaagcgaa actccgatct cgggcttacc gccaggccaa gagagcctcg
     1681 cttgccgggg atgtgcagtg gattgggcgg gaagaagatg cgcgtcatct ccagaataca
     1741 ggtgacgcat gatggggcga acgcattgcc tggtgccagc cgcgctggca gcctggcttt
     1801 ttggaccaga ccccacgatt ctgttgatct cagcaggaac tgggctcttg ccggacattg
     1861 atgagccgca atcgaccatt gggtatcgat tgtttctctt ggccaaagga ttcaaagggc
     1921 tggtaggcca tcgcacctat agccattcgc tggttggaat cgtgactgcc gccgctctgg
     1981 ccgtcatctt tgatttgcca tggatatgga tcgaagcggg cttgatcggc tgggtgagtc
     2041 atcttgtgtt ggatgcactg agcgggggtg tgccgttatg gtggccattg tatgcgacga
     2101 atcgagagcg gtgggtgttg gcgcgatgga agccgtttgg gtttgccgat catctgatct
     2161 tagttctggc aggggtagcg ttgggcgcag cggcgattca tggaaactgg ctggggtggt
     2221 ggatcgagct cctgaagcgg gatatcagcg tgtcgcaatg agagcgggac tgccggcgcg
     2281 agcgtatgct cgcgccggcc ctacggccac agcgtgtatc gccgtggaat agaggccgtg
     2341 gccctgccgt cggtgaacca ttccggaaag cgcgcgagcg catactcaat agccacatga
     2401 ctatggcaga agactccagg gggcagtagc agaggaatgt gtaggtaggc gtcgcggcga
     2461 gcccctgccg aaggcctctg cttgtgcggg agcgacgtgg agaagagcgc ggacagcacc
     2521 gcgacgatag gatcgctatg gccattggta ctgtccgccg tgcgctgtcg gtcttcttgt
     2581 ggaagaagac agctacgcta aaataaaggt g
//
LOCUS       DAORRQ010000182         2609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_911_length_2609_cov_33.452623, whole genome shotgun sequence.
ACCESSION   DAORRQ010000182 DAORRQ010000000
VERSION     DAORRQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_2609_cov_33.452623"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1487
                     /locus_tag="PK224_21355"
     CDS             144..1487
                     /locus_tag="PK224_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRB18352.1"
                     /translation="MDTALQYDRLQFAVTATFHYLFPQLTMGLALLLVYLRTRDLLSE
                     GDHYHQVARFWTGIFALSFAFGVVTGIPLEFQFGTNWAKFSEYAGGIVGQTLAMEGVF
                     AFFLESSFVGLLLFGESRVSRRTLWLATVLLWLGSWLSGYFILATNAWMQHPVAYHVA
                     ADGRLTVESLVGLLTNPWLIWQFAHNMTAAVVTGAFVVAAVGALYQLAGKHPALARTC
                     LRTGVVAGAFASVLLITPTGHESARQVFEFQPVKGAAFEGLFRTERGADLVLIGQPNM
                     ETMTIDNPLVVPSALSLLAYDDLYAKVKGLDAFPPREWPDHIALLYYAYHIMVGLGTL
                     LLAVMGLALAWLWRGRLFTARWLLWLLLLSAPFPYVATTAGWMTAELGRQPWLVYGLF
                     RTEEGISPLVHSGNALFTLLGFLGIYLGLGLLFVLLFLEILRHGPTEPAVGQAGS"
     gene            1491..2540
                     /gene="cydB"
                     /locus_tag="PK224_21360"
     CDS             1491..2540
                     /gene="cydB"
                     /locus_tag="PK224_21360"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRB18353.1"
                     /translation="METIWYALVLFVLTTYVVLDGYDFGVGILSPFVARQESERRLVR
                     ETIGPVWTGNEVWVIAGSALLFLAFPKAFASGFSGFYLALMIMLWLLMGRGLAFELRE
                     HVDHALWRTFWDTIFFLANLLLAFVVGLVVGNLLRGVPLNASGYFFLPLWTDFGPGSM
                     PGILDWFTLLTGLTLVALLTLQGASFLVMKTRGELQARAEALAKSATVLLVLLLVAVV
                     VALLSVQPAFGAHYAAAPIGFVWPLTAVVALLVRHVSAVRQRSSLGFVASSVSVASLL
                     VAAAWGIFPNLLIATTGQANSLTSLNAATDRTGLEAGLWWVPPGLALVILYQVQIHRL
                     FARPAKPESPAMARH"
BASE COUNT          362 a          765 c          857 g          625 t
ORIGIN      
        1 tgtcgtgcca tgatgcaaaa cctcctctat agatacaagg tcttgcaatc gaatacttca
       61 tcggccctgg ggaagtcact agcttcccca tcaggtctga tcagtcacaa tgtcgcgggc
      121 cagttcaagg gggtggggta gtgatggata ccgcgctgca atatgaccgg ctgcaattcg
      181 ccgtcacggc cacgtttcac tacctctttc ctcagctcac gatgggactc gccctgctgc
      241 tggtgtatct gcgcacgcgt gacctgctca gtgaggggga ccattatcac caggtcgcgc
      301 gattctggac cggcatcttt gcgctgagtt tcgcgttcgg cgtcgtcacg ggcattccgc
      361 tcgaatttca gttcggcacc aactgggcca agttctccga atacgcaggc ggcatcgtcg
      421 ggcagacgct ggcgatggaa ggggtgtttg ccttttttct ggaatcgtcg tttgtcggcc
      481 tgctcctgtt cggcgaatcg cgcgtcagcc ggcgcacgtt gtggctggcg acggttttgc
      541 tatggctcgg ttcctggtta tccggctatt tcattctggc gacgaacgcc tggatgcaac
      601 atcctgtggc ctatcacgtg gcggccgacg gccgtctgac ggtcgagagt ctggtcgggc
      661 tgctcacgaa tccctggctg atctggcagt tcgcccataa catgacggcg gcggtcgtca
      721 ccggcgcgtt cgtggtggct gcggtgggtg ccctctatca actggcgggg aaacatcctg
      781 cgctggccag gacctgtctg cgcacgggcg ttgtggcggg cgcgttcgcc tcggtgctgc
      841 tcatcacacc gacgggacat gagagcgcgc gacaggtgtt cgagttccag ccggtcaagg
      901 gcgctgcctt cgagggcctc tttcgtaccg agcgcggcgc cgacctcgtg ctgatcggcc
      961 agcccaacat ggaaaccatg acgatcgata atccgctcgt cgtgccgtcg gcgctgagcc
     1021 tgctcgccta tgacgatctt tacgcgaagg tgaaggggct ggatgccttt cccccgcgcg
     1081 agtggccgga ccacattgcg ctgctgtatt acgcgtacca catcatggtc gggctcggca
     1141 cgctcttgct cgcggtgatg ggattggccc tggcgtggtt gtggcgtggc cggctcttca
     1201 ctgcccgttg gttgctctgg ctcctgctgt tgagcgctcc ttttccctat gtcgcgacga
     1261 cagccggctg gatgacggcg gagcttggcc gccaaccctg gctggtgtac gggctctttc
     1321 gcacggagga gggcatctcg cccttggtcc attctggcaa tgcgctcttc accttgctcg
     1381 gcttcctggg aatctatctc gggttggggc tgctgttcgt cttgttgttt ctggagatcc
     1441 tgcgtcacgg gccgacggag ccggctgtgg ggcaggcggg gagctaacac gtggaaacaa
     1501 tctggtatgc cctggtgctg ttcgtgttga ccacctatgt ggtgctcgac ggctacgact
     1561 tcggggtcgg aatcctgtcg ccattcgtgg cccggcagga gagcgaacgc cgcctggtgc
     1621 gggagacgat cggaccagtg tggaccggca acgaagtgtg ggtgattgcg ggcagcgctc
     1681 tcttgttcct ggcctttccg aaggcctttg cgtcggggtt cagcgggttc tacttggcct
     1741 tgatgatcat gctgtggctc ctgatgggca gggggttggc tttcgagctg cgggagcatg
     1801 tcgatcacgc gttgtggcgc acgttctggg ataccatctt tttcctggcc aatctgctgc
     1861 tggccttcgt ggtgggtctg gtggtgggga atctgctgcg gggagtgccc ttgaacgcga
     1921 gcggctattt cttccttccg ctctggaccg acttcggccc cggttcgatg cccggcattc
     1981 tggattggtt cactctgctg acggggctga ccctggtcgc tttgttgacg ttgcaggggg
     2041 cgagtttctt agtgatgaag acgcgcgggg agttgcaggc aagggcggag gcgctggcga
     2101 agtccgccac cgtcttgctg gtgctgttgc tcgtggccgt ggtcgtggcg ctgctctccg
     2161 tgcagccagc gttcggcgcg cattatgcgg ccgctcccat cggctttgtc tggcccctga
     2221 cggcggtggt cgcgctgctg gtccggcacg tgagcgctgt ccggcaacgg tcgtcccttg
     2281 gcttcgtggc gtcgagtgtg tcggtcgcga gcctattggt tgcggcggct tgggggattt
     2341 tccccaatct gttgattgcg acgaccggac aggccaacag tctcacgagc ctcaacgccg
     2401 caaccgaccg gaccggtctc gaagccggtc tctggtgggt gcccccgggc ctggccttgg
     2461 tgatcctgta tcaagtgcag attcatcgcc tcttcgcccg cccggccaag cctgaatctc
     2521 ctgcgatggc gcgccactag tctagtcttc cctttttggg gtcagcaact gtcttgaaaa
     2581 tcaagccgac tgactgaccg cgacccgcc
//
LOCUS       DAORRQ010000183         2560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_925_length_2560_cov_5.553693, whole genome shotgun sequence.
ACCESSION   DAORRQ010000183 DAORRQ010000000
VERSION     DAORRQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_925_length_2560_cov_5.553693"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    257..278
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<279..754)
                     /locus_tag="PK224_21365"
     CDS             complement(<279..754)
                     /locus_tag="PK224_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18354.1"
                     /translation="MAIHESRRHVIAVETPSGAYKIFALGYNKRDGSLFIDLPYLKCD
                     GLVGAATGIRNEAGGLSDRDFIPFGTTKYGIKFSYHPDGRTHFSQDGKIRTNLSHNGM
                     ALSKKTGPICTIIAHRLCEFTRVSQQDLRKDSTKKGICALISSVELPGAVFTVFLKS"
     gene            complement(902..1939)
                     /locus_tag="PK224_21370"
     CDS             complement(902..1939)
                     /locus_tag="PK224_21370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRB18355.1"
                     /translation="MSTHQDRIETALLADDWDRVGYEASEWARALETAGEKDPRPYFA
                     LNVTHLIRGEFADAWRVYARALQETDDIERVREWVHSIADRHAGNAQTHLVQGLFLAQ
                     SGQSEQSMASYKEAAKLAPQSPYPHYFLAQIHERAAHLEMAIKEYREAVKLAPTFAPA
                     RTNLGVAYQEQGRLEMAIPQYREVIKLNPHDALAHANLGCALAEQGKFEPALQAYKEA
                     LRLNPSDAEIHFALGGVYETKGRTDLALKEYREATQLNPELASAHTALGWLLMEQSRA
                     SEAMEAFSKAVKANPEEAQALYGIGRIYAAKGKRESAAENFSKAIRFEKDPTKKNAIM
                     NALFASGQTGD"
     gene            complement(1959..>2560)
                     /locus_tag="PK224_21375"
     CDS             complement(1959..>2560)
                     /locus_tag="PK224_21375"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRB18356.1"
                     /translation="EVVTTLLYRASQAPYRKILAVVRDWTEKQKQDTIEIAFQKRGPH
                     DELIKEFRSGYAFVFDVMMDIGGWRDMHRHRRCQQVQQNFTTVHGFETPPILAEAGLD
                     HEYREAMGHVKSDIEQLRKSSQEAAMYAIPFGFSVRCLFKMDYAEAEYIAKLRSGVKG
                     HWSYRTIAWLMKQKLTERYPILGARMQATPPDVQDALTR"
BASE COUNT          501 a          717 c          729 g          591 t
ORIGIN      
        1 atagacaact gattgggtga caagtttgcc tttagggata gcctcatctt gagtttctac
       61 gtatggacct tggaagagca agtatgattc agggactatc gaggctacac taagcggtcg
      121 atatgcaact atcaggaccc tatcatgaca tctgtccatc aagggctgtc cgatcgcaat
      181 ggctgggaga gcgatggtct cagttggcgt tgtcatctcc ccgactggag aacctacacc
      241 cgtggagggt gacgacnnnn nnnnnnnnnn nnnnnnnnga tttcaagaaa accgtgaaaa
      301 ctgctccggg caactcaaca gatgagatca atgcgcagat ccccttcttg gtggaatctt
      361 ttcgcagatc ttgctggctt acacgggtaa actcgcataa gcgatgtgca ataatggtgc
      421 aaatgggacc cgttttctta gagagtgcca taccattatg tgaaagattc gtgcgtatct
      481 ttccgtcttg ggagaaatgg gtacgcccat ctggatggta ggaaaacttg atgccatatt
      541 tggtagttcc aaaaggaatg aagtcgcgat cagaaagacc acctgcttca ttacgaattc
      601 cagtggcggc accgactaaa ccatcacact tcaggtacgg cagatctatg aacaaactgc
      661 cgtccctttt gttgtacccg agtgcaaaga tcttgtaagc acccgaaggt gtttctaccg
      721 ctatgacatg tcgccttgat tcgtggatag ccatgaactg ttagtggtcc agggtttggg
      781 caacaaccgt ccacataacc tctccagagg agctgagatg atgtgcttcc cctgcgcgcg
      841 tcgatcgagg tccttccaag ggcgcgcgat ccgcgagcaa ggaagcacat ccctcggctg
      901 ctcaatcccc tgtctggccg ctggcgaaga gggcgttcat gatggcgttt tttttggtgg
      961 gatctttttc aaagcggatg gctttggaga agttttcggc ggcactttcc cgcttgccct
     1021 tggcggcgta aatcctcccg atgccgtaga gggcctgggc ttcctccggg ttggccttca
     1081 cggctttgct gaaggcctcc atggcctccg atgctctcga ttgctccatc aacagccaac
     1141 caagcgcggt atgggccgag gcgagttccg gattgagctg cgtcgcttcg cgatattcct
     1201 tgagggcaag atcggtccgc cccttggtct cgtaaactcc gccgagcgcg aaatggattt
     1261 ccgcgtcgct cgggttgagc cttagggctt ctttgtaggc ttgcagcgcc ggttcgaatt
     1321 tgccctgctc cgcaagcgca cagccgagat tggcatgggc cagcgcgtcg tggggattca
     1381 gcttgatcac ttcgcggtat tggggaatgg ccatttcaag ccggccctgc tcctggtagg
     1441 ccacgccgag gttcgtgcgc gccggcgcaa atgtcggagc cagcttgacc gcctcgcgat
     1501 attccttgat cgccatctcc aaatgcgccg cccgctcgtg aatctgggcc agaaaataat
     1561 ggggataggg cgattgcgga gccagcttcg cggcctcttt gtaggacgcc atcgactgtt
     1621 ccgactggcc ggactgcgcc agaaacaatc cctgcaccag atgcgtctga gcattgccgg
     1681 cgtgccgatc cgcgatggag tgcacccact cccggacgcg ctcgatgtcg tccgtctcct
     1741 gcagcgcgcg ggcatacacc cgccaggcgt cggcgaattc cccgcgaatc aggtgggtga
     1801 cgttcaaggc gaagtacggg cgaggatctt tttcaccggc ggtctccagc gcgcgggccc
     1861 attccgacgc ctcatacccg acccggtccc agtcgtctgc caggagggcc gtttcgattc
     1921 tgtcttggtg cgtgctcatg atgaaagagg cgcgccgctc agcgcgtcag agcgtcctgc
     1981 acatccggcg gagtcgcctg catgcgcgcg ccgagaatgg gatatcgttc ggtcagtttc
     2041 tgtttcatga gccaggcgat ggtgcgatag gaccagtgcc ccttcacccc ggaacgcaat
     2101 ttcgcaatgt attccgcttc cgcatagtcc atcttgaaga ggcagcgcac ggaaaatccg
     2161 aacgggatgg catacatggc cgcttcctgg ctcgatttcc ggagttgttc gatatcggat
     2221 tttacatgcc ccatggcttc gcggtattcg tggtcgagcc ccgcttctgc caaaatgggc
     2281 ggtgtctcga acccgtgcac agtggtgaag ttctgctgca cctgctggca gcgccggtgc
     2341 cgatgcatgt cgcgccaccc gccgatgtcc atcatcacat cgaacacaaa ggcatagccg
     2401 ctgcggaact ccttgatcaa ttcgtcatgc ggtccgcgtt tctggaaggc gatttcgatg
     2461 gtgtcctgct tctgtttctc cgtccaatcc cgcacgaccg cgaggatctt tcgatacgga
     2521 gcctgcgagg cgcgatagag cagcgtggtg acgacttcgt
//
LOCUS       DAORRQ010000184         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_967_length_2445_cov_32.804603, whole genome shotgun sequence.
ACCESSION   DAORRQ010000184 DAORRQ010000000
VERSION     DAORRQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_967_length_2445_cov_32.804603"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PK224_21380"
     CDS             complement(<1..820)
                     /locus_tag="PK224_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRB18357.1"
                     /translation="MSQKTCLRETLYSLVGWGIGEDDIAGAILRVPCWPWGFFYLRAV
                     RIVSSRRFAAACIGVVASVLLACLAFAFGETQEDHTVMKRRQQGILTGMPFMANITPR
                     TFIDDAGRKLYVAKAPTRVVSLAPSITEMLFALGLDEQIVGVTEFCDFPAAAKAKSKV
                     GYANPNLESLVALRPELIIAPREFHRANVLAKLEELKIPVLLLEATSVENIFSHIHTL
                     GRIFDRSTEAHAMTAAMRSRMAEISSRTEHLPRIRVLYVINSQPLITVGPGSYIH"
     regulatory      complement(784..966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1066..1323)
                     /locus_tag="PK224_21385"
     CDS             complement(1066..1323)
                     /locus_tag="PK224_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRB18358.1"
                     /translation="MDQVTLIGLLAGTLTTIAFIPQLQQTWRTRSAQDVSLGMLLTFV
                     TGVFLWLIYGLMLGALPIILANLVTLVLTLAILILKLRYRS"
     gene            1482..1673
                     /gene="rpmB"
                     /locus_tag="PK224_21390"
     CDS             1482..1673
                     /gene="rpmB"
                     /locus_tag="PK224_21390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRB18359.1"
                     /translation="MAFACDLCGKKHQTGNNVSHANNKTKRVFNPNLQRVKALVNGSA
                     LRIRVCTRCLRSGLVKKAV"
     gene            1932..2306
                     /locus_tag="PK224_21395"
     CDS             1932..2306
                     /locus_tag="PK224_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18360.1"
                     /translation="MIKISRSLSLIIISMLLAACSHFQNREAAYLDSAKGWATQAQVR
                     EKLGAPKTVRQDEGGATVWVYELREQQSGNRLTAPGMWCEHYLLTFDDKAVLQKWRQV
                     SHFHGGELVPQECIPGSEPEKS"
BASE COUNT          567 a          712 c          703 g          463 t
ORIGIN      
        1 ggtggatgta actgccggga ccaaccgtga tcaagggctg gctgttgatc acatacagca
       61 cgcggatgcg cggcaggtgc tcggttcgac tgctgatctc ggccattcga ctccgcatgg
      121 ccgcggtcat ggcatgggct tccgtcgaac gatcgaaaat gcgtcccagc gtgtgaatgt
      181 gcgaaaaaat gttctccacc gaagtggctt ccagcaggag caccggaatc ttcaattcct
      241 ccagcttggc caacacattc gcgcggtgga actcccgcgg cgcaatgatc aattccggcc
      301 ggagggccac gagggattca agattggggt tggcgtaccc gaccttggat ttagccttgg
      361 ctgctgccgg gaaatcgcaa aactccgtga ccccgacgat ctgttcgtcc agccccaacg
      421 caaacaacat ctccgtgatg ctcggcgcca atgacacgac ccgtgtgggc gccttggcga
      481 catagagttt tctgcccgca tcgtcgatga aggtgcgcgg ggtaatgttg gccatgaacg
      541 gcatgcccgt aaggatgcct tgctgacgac gtttcatcac cgtgtgatct tcctgggtct
      601 ctccgaatgc gaaggcaagg caggccagca gcacagacgc gaccacgccg atgcatgcgg
      661 ctgcgaaccg gcgactggag acgattcgaa cggcgcgcaa ataaaaaaat ccccagggcc
      721 aacatggaac cctgaggatt gcacccgcaa tatcatcctc gcctattccc cagcccacga
      781 gggaataaag ggtctccctc aggcaggtct tctggctcat ggttcatcct actccccgcg
      841 ccttcccact caccgaacgg tgaacagtgg cgcctgcggg tttcgtcccc atttacagcg
      901 gcgggaccgc gagggttttt caccctcttc ccttgacccg aggtgtcttg aaaagagaca
      961 aactttagga gcgcatggcc gatcttgtca agttcaaact tgccgtcacg acgttcgatg
     1021 gagcagcata ggatattgaa ctgcatacca aacaccctgc agcccctacg agcgataccg
     1081 gagcttcagg atgagaatcg ccagggtcaa caccagggtc acgagattcg ccagaataat
     1141 gggcaaggcg ccgagcatga gcccatagat caaccagaga aagaccccgg tcacgaaggt
     1201 gagcaacatt cccagcgaca cgtcctgggc ggaacgagtg cgccaggtct gttgcagctg
     1261 aggaatgaac gcgatggtgg tgagggtccc tgccagcaat ccgatgagcg tgacctgatc
     1321 catatctgca acctcagtgg agtgagcggc gcgagcggga gagggcatcc tggtagggag
     1381 aattcacctc agcagcttgt catgacctgt gacgctatgg tagcatcccc tcccatccaa
     1441 ggactagtcg ttgaaagacg ccgcaacaaa ggagtagggc cgtggcattt gcctgtgatc
     1501 tctgtgggaa aaagcatcaa accggtaata acgtcagcca cgcaaacaac aagacgaagc
     1561 gcgtcttcaa tcccaacctg cagcgagtga aggcgctcgt gaacgggtcg gccttgcgca
     1621 ttcgggtttg cacgcgttgc ctgcggtccg gcctggttaa aaaagccgtc tgattgctcc
     1681 gcatttctcc cccatctcgt cagctcgtga tctgatctca cgcctaagtt gcgccttctc
     1741 cccgctcgct cttcttcaga ttcggtacgg cctcacacac ccccacgaat agtctgccgc
     1801 ctcggactca cggagaccgg ggacgatgca ggagatcttc tccaaaggag acgcgccccc
     1861 gctatccgtg tacaatcccc agccagacaa gcgggtgaaa aaaacggatc ccagtcgaat
     1921 cgggaggcaa catgataaag atcagtcggt cgctcagcct catcatcatc tccatgctgc
     1981 tcgcggcatg cagccatttt cagaaccggg aagcggccta tctggattcc gccaaggggt
     2041 gggccactca agcccaggtg cgggagaaac taggcgctcc caagactgtt cgacaggatg
     2101 aaggcggagc caccgtgtgg gtgtatgaat tgcgcgagca acagtccggg aaccgtctga
     2161 ccgcacccgg aatgtggtgc gaacactatc tcctcacctt cgacgacaag gccgtgctcc
     2221 agaaatggag acaggtgtcc catttccatg gcggcgaact cgtgccacag gaatgcattc
     2281 cgggtagtga gccggagaaa tcctagggaa agcgcagcga ggatggacgc aatactctgc
     2341 tctctcgcgc gcttatccgc gaggctgcgg aatacacaga atatgatgga gttgggggga
     2401 cgttggtaac aggagcaacc cgaggaagaa atggtgccga agcgg
//
LOCUS       DAORRQ010000185         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_985_length_2391_cov_20.215325, whole genome shotgun sequence.
ACCESSION   DAORRQ010000185 DAORRQ010000000
VERSION     DAORRQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_2391_cov_20.215325"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PK224_21400"
     CDS             complement(<1..860)
                     /locus_tag="PK224_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRB18361.1"
                     /translation="MRLPVDPMCTADVAAGVYSDRHHAYDVQGASFCYGKLSGRESRW
                     VLSDVSLHVEPGEILGIVGPNGSGKTSLVKLLAKLAVPQRGTLSLFGRNLADLSREET
                     ARTVAFVPQESPPAFSFTVAETVLMGRYPHRRQTRWSLGFGWEDREDCAAAAQAMATM
                     DIGHLASRAVTDLSGGERQRTMIARALAQTPRVLLLDEPTAFLDLQHQLEICSVLRRL
                     TDEQGLTVVIVSHDLNLASQYCDRIVMLKEGSMYSMGTPAEVLSVEVLRAVYGCEVLI
                     DPHPESGLPR"
     gene            complement(857..1927)
                     /locus_tag="PK224_21405"
     CDS             complement(857..1927)
                     /locus_tag="PK224_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRB18362.1"
                     /translation="MTGYERPFQGAILTPVRWCVIMGSLSVVALILAVVCLQLGTQYI
                     GLGKLISVLSSALLDQPTDNDVLKTTSVILLQVRLPRVFLGFLVGVCLASVGVALQAL
                     LRNPLADPYVLGVSSGAALGVAVAVLFGIGTTVLAFSLLPVCGFLGGLVALLVVYRMA
                     ATYDRLPIHSVLLAGVILNAIFSALIMFITSIMEPNRSFGMMAWLMGSLTGPAYPALA
                     ALSVYLLAGLFLLFKQVRVLNILALGEEPARSLGIDTERAKRVIFLVSALLTGAVVSV
                     SGMIGFIGMIIPHAVRLVIGADHRLLLPASALVGGIFLMVADTMARTFFVPSEVPVGI
                     ITALAGGPFFVYLLVWRKDRLV"
     gene            complement(2014..>2391)
                     /locus_tag="PK224_21410"
     CDS             complement(2014..>2391)
                     /locus_tag="PK224_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRB18363.1"
                     /translation="YVINSQPLITVGPGSYIHQMIGLAGGTNIASGAATPYPRLTMET
                     VLKEDPEVLIFPMGSVETVPRREQQEWRRWTTLTAVQQNRLREVSANALNRPGPRVME
                     GLEELVRVIHPEAFPPESAPVQP"
BASE COUNT          541 a          765 c          704 g          381 t
ORIGIN      
        1 atcctgggca gtcccgactc cggatgcgga tcgatcaaga cctcacagcc atagaccgcc
       61 cgcaatacct ccaccgacaa cacctcagcc ggcgtgccca tcgaatacat cgacccttcc
      121 ttcagcatga cgatccggtc gcaatactgg ctggccaggt tcaaatcgtg cgacacgatg
      181 acgacggtca ggccctgctc gtcggtcaaa cgacgcagca ccgagcagat ctcgagttga
      241 tgctgaagat cgagaaaggc ggtcggctca tcgagcaaga ggacccgggg cgtttgggcc
      301 aaggcccgcg cgatcatcgt acgttgacgt tcaccgccgg acaggtcggt caccgcacgc
      361 gaagccagat ggccgatgtc catcgtggcc atggcctgag cggccgccgc acaatcctcc
      421 cgatcttccc agccgaaccc gagactccac ctggtctgtc ggcgatgcgg ataccgtccc
      481 atcaacaccg tttccgccac cgtaaaggag aaagccggcg gactttcctg cgggacgaac
      541 gccacggtcc gcgccgtctc ctctcgtgaa aggtcagcca ggtttcttcc gaagagcgag
      601 agggttcccc gttgaggcac ggcaagcttg gccaggagtt tcacgagtga cgtcttgccc
      661 gatccattgg gcccgacgat accgagtatt tcgcctggct cgacgtgcag gctcacatcg
      721 ctgaggaccc accggctttc ccgcccagac aacttgccat agcagaacga ggccccctgc
      781 acatcgtagg catggtgccg gtccgagtag acaccagccg caacatcagc cgtacacatc
      841 gggtctacag gaagcctcat acgagccgat cctttcgcca gaccagcagg tagacaaaga
      901 acgggccgcc cgcgagggcc gtgatgattc cgaccgggac ttccgacggc acgaagaatg
      961 tcctggccat ggtatcggcc accatcaaaa atatgccgcc cacgagcgct gaagccggca
     1021 ggagcaatcg atgatccgcc ccgatcacca ggcgcaccgc gtgaggaatg atcatcccga
     1081 tgaatccgat catgccgctg acggacacca cggcgccggt gagcagcgcc gacacgagaa
     1141 agatcacccg tttggcgcgt tcggtatcga ttcccaacga ccgtgccggc tcttcaccca
     1201 aggccaggat gttcaggacc cgcacctgct tgaaaagcag gaagagaccg gccaacagat
     1261 agactgagag cgcagccaac gcgggatagg ccgggcccgt gagcgatccc atgagccagg
     1321 ccatcatgcc gaaggaacga ttcggttcca taatcgaggt gatgaacatg atcagcgccg
     1381 agaaaattgc gttcaggatc acaccggcca gcagcacgga atgaatcgga agccggtcgt
     1441 aggtggccgc catacgatag accaccagca aggccaccag tccgcctaaa aacccgcaga
     1501 ccggcaggag ggaaaatgcg aggaccgtag tcccgatccc gaacagcacc gcgaccgcca
     1561 cacccagcgc ggctccactg gacaccccga gcacataggg atcggccaga ggatttctga
     1621 gcagggcctg cagcgccacc ccgaccgacg ccaggcagac tcccacgaga aatcccagaa
     1681 agactcgcgg cagacgcacc tgcagtagga tgacgctggt cgtcttgagc acgtcgttgt
     1741 cggtaggctg gtcgagcagc gcggacgaca gcacgctgat aagtttcccg agtccgatgt
     1801 actgtgtccc gagttgcaga cagacgacag ccaggatcag cgcgacgacc gacaaactgc
     1861 ccatgatgac gcaccaacgc accggagtca ggatggcccc ctgaaacggc cgttcatatc
     1921 cggtcattgg agcggaactg tgacggctcg tggaaggcaa cgggggcgag gccaatgctc
     1981 gagcaggtga cgtggagtgg ccgaccgaag gcatcatggc tggaccggtg ccgattcggg
     2041 aggaaaggcc tcggggtgaa tcaccctcac gagttcttcc aacccttcca ttacacgagg
     2101 cccgggacga ttgagcgcat tggccgaaac ctcgcgcaac cggttctgct gcacagcggt
     2161 cagggtcgtc cagcgccgcc actcctgctg ttcgcgtcga ggcaccgttt caaccgaccc
     2221 cattgggaag atcagcactt cgggatcttc tttcaggacc gtttccatgg tcagccgtgg
     2281 atagggtgtt gcagctccgg aggcgatatt ggttcccccg gccaggccga tcatctggtg
     2341 gatgtaactg ccgggaccaa ccgtgatcaa gggctggctg ttgatcacat a
//
LOCUS       DAORRQ010000186         2384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_988_length_2384_cov_13.300988, whole genome shotgun sequence.
ACCESSION   DAORRQ010000186 DAORRQ010000000
VERSION     DAORRQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_988_length_2384_cov_13.300988"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK224_21415"
     CDS             complement(<1..225)
                     /locus_tag="PK224_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRB18364.1"
                     /translation="MSGPVPVRIVRSAARAIQEAAEWWVVNRPKAADGFTVELERAIQ
                     LLSSQPTIGARALNARLTGVRRIHLTRIHYY"
     gene            complement(222..440)
                     /locus_tag="PK224_21420"
     CDS             complement(222..440)
                     /locus_tag="PK224_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18365.1"
                     /translation="MLITTGKVLGGIIKLDEKSLPEGAIVTVLAPEGDETFELRPEEE
                     VQLLAAIAEAERGETTDASKVLKQIPRS"
     gene            complement(543..788)
                     /locus_tag="PK224_21425"
     CDS             complement(543..788)
                     /locus_tag="PK224_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18366.1"
                     /translation="MPNSRVCYSGATSDLAVCVPLSRIGQHAPNVSPEISGALYHVIV
                     RGNARHTIVLDDRDRQRFLDVLARVVDRFHLPTLCRA"
     gene            822..1040
                     /locus_tag="PK224_21430"
     CDS             822..1040
                     /locus_tag="PK224_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18367.1"
                     /translation="MAQRLMEPLTVMESFDVLRDGDLGLGLTLDEEKGSGVFIGASFC
                     GSGTRSFEFWTGNLVIHTIRLPESLVKQ"
     gene            complement(1030..1353)
                     /locus_tag="PK224_21435"
     CDS             complement(1030..1353)
                     /locus_tag="PK224_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18368.1"
                     /translation="MEAKTLYLIWLNVSDSRRRGYARGGRGMRERLMAACRPGGTVLS
                     VLVCESLECFLIDREIFALSNGDDFDQDLLVDDPVHDTDRFLRCVEFVIAGEIAERKG
                     SRVIV"
     gene            1567..2289
                     /locus_tag="PK224_21440"
     CDS             1567..2289
                     /locus_tag="PK224_21440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18369.1"
                     /translation="MRPGSGYTIAGHPLDDRDPQYTVNDYRTYVIAGCVPKHLPIKGP
                     VGSVWVACNDLDYAKDKVVESRFVSCLEMFPGGVLQGLFIKEVNNRTWESFRFSCREL
                     QADGTVGFRWEKSPFLFNYEKEGDLFETTVPTNRLSIGLYEVYNKLQLRESLLQVGLE
                     HATAQDIHENGLKGKDPQSIQVSPRVPDAFGLGGIAGTHKWECPPGMVVTGAAIGHVP
                     DNKGKHTRPVYLLMECRKLFHG"
BASE COUNT          503 a          705 c          694 g          482 t
ORIGIN      
        1 atagtaatga atgcgggtga gatgaatgcg gcgaaccccg gtcagcctag cgttgagggc
       61 tcgtgcgccg atggtcggct gggatgaaag aagttggatc gcacgttcca gctcgaccgt
      121 aaagccatcc gcagcctttg gtcgattaac gacccaccat tcggcagctt cttgaatggc
      181 ccgggcggcg ctccggacga tacggacagg gactgggccg ctcacgatcg agggatctgc
      241 ttgaggactt tcgaggcgtc ggttgtctca cctcgctccg cctccgcaat cgcagcgagt
      301 agctgaacct cttcttccgg tcgcaattcg aaggtttcgt caccctccgg agccaacacc
      361 gtcacaatcg ccccttccgg cagacttttc tcgtcgagtt tgattatccc gccgagaact
      421 tttccagtgg tgattagcat agcggcatcc taccatttca tgctgtgatc gtccaacagc
      481 ggatgggaag ggtgtggctg tacgctcccc gatcacaatc aatgcagagc ttccttctga
      541 cgttaggctc gacagagggt gggaaggtga aagcgatcga cgacacgcgc cagcacatcg
      601 agaaacctct gccggtcccg gtcatcgagc acgatggtgt gccgggcgtt cccgcggacg
      661 atgacgtggt acagcgcccc ggaaatttca ggagaaacat tcggggcatg ctgcccaatc
      721 cgactcagcg gcacgcacac tgcaagatct gaggtggccc cggaataaca gacacgtgaa
      781 ttaggcacca ctctcctgtt gctcgaactc ctccggcttc gatggcccag cgcttgatgg
      841 aaccgctcac ggttatggaa tcgttcgatg tactccgtga tggcgacctg ggcctcggcc
      901 tgactctgga cgaagaaaag gggtcgggag tctttattgg agcatcgttt tgcggctccg
      961 gcacccgcag ctttgaattc tggaccggca acctcgtcat tcacacgatc aggttgccag
     1021 aatcgctcgt caaacaatga ctctcgaccc ctttctttcc gcgatctcac cggctattac
     1081 aaattctacg cagcgaagaa atcgatccgt atcgtgtacc ggatcatcga ccaaaagatc
     1141 ctggtcgaag tcgtcgccat tggaaagcgc gaagatctcg cggtctatca ggaagcactc
     1201 aagcgattca cacaccagga ccgatagaac tgttcctccg ggcctgcatg cggccatcag
     1261 tctctccctc atccccctac cgcctctcgc atatccacgg cgtctcgaat cgctcacgtt
     1321 cagccagatc aggtacaggg tcttagcctc cattcctgtt ccattacatt tcgtccatgt
     1381 cacgagcctg ggcggccccg ccagcctctc gccagagcgg cgcaactgac gtacaatccg
     1441 acccgtcaca acgaggaggg atgtcccatg accataggaa cgtgtgcgcc gcgtgtcggg
     1501 cttctggttg cgctgggagc cgcgctgctg ctgccggcct ccacaccgtg agccgggacg
     1561 ggcgccgtgc gaccgggaag cggctacacc atcgccggcc atccgttgga tgaccgcgac
     1621 ccgcagtaca cggtgaacga ctatcgcacc tacgtcattg ccggctgcgt gccgaagcat
     1681 ctgccgatta aggggcccgt cggttccgtg tgggtggcct gtaacgattt ggattacgcg
     1741 aaggataagg tagtcgagag ccggttcgtg tcctgcttgg agatgtttcc gggcggcgtg
     1801 ttgcaagggc tgttcatcaa agaggtgaac aaccgcacgt gggaatcgtt ccggttctcc
     1861 tgtcgggaat tgcaggccga cggcacggtc gggttccgtt gggagaagag cccgttcctc
     1921 ttcaattacg aaaaagaggg cgacttgttc gagaccacgg tgccgacaaa ccgcctttcc
     1981 atcggcctgt acgaggtcta caacaagctg caactccggg agagtctcct gcaggtgggg
     2041 ctggaacatg ccaccgcgca ggatatccac gaaaacgggc tgaagggaaa agatccgcaa
     2101 tccatccagg tcagtcccag ggtgccggat gcgttcggac tgggaggcat cgcgggcacg
     2161 cacaaatggg aatgtcctcc gggcatggtg gtgaccggcg ccgccatcgg ccacgtgccc
     2221 gataacaaag gaaaacacac ccggccggtc tatctcctca tggagtgccg gaaactcttt
     2281 cacggataag gaagagtcgg aggaggaaaa tgggacaagg aaatatttaa ctatgtcgtg
     2341 gccggggtta acccgtgcgg tgtcacgaag cagcgtccct ccac
//
LOCUS       DAORRQ010000187         2379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_993_length_2379_cov_2.753012, whole genome shotgun sequence.
ACCESSION   DAORRQ010000187 DAORRQ010000000
VERSION     DAORRQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_2379_cov_2.753012"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="PK224_21445"
     CDS             <1..1619
                     /locus_tag="PK224_21445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HRB18370.1"
                     /translation="RADTIFEAMEPLQSLYADHNELSFYTWSNQRCCVPTGAVSATLA
                     GHHPHLTPGSILLFEEVLGPETGAAADADPARRHAVRLDRIVATTSGGTPLTDPVTAQ
                     LVTEITWHDDDALPFPLCLSAATSAGYRDGVSVARGNLLLADHGVTLAEEALGVVPEP
                     FLSMPRSKEEDRCTPCSPRMIPPRFRPGLTKAPLTHAGPAYDHAKSAWAAMQWALRDV
                     QPAITLTGTKESETTTWTARRDLLNSDAAADEYVVEIENDGGGAIRFGDDVHGRRPES
                     GTDFTARYRVGNGRAGNIGADSLVHIAIGIPEITRVRNPLPAIGGQEPESLQDVRQRA
                     PVAYRIQERAVTPVDYAEVTERRADVQRAAATFRWTGSWHTVFATVDRGGGVAVTGEF
                     EADIRAHIERYRMAGHDVEIDGPQFVALEIELHVCVAPEHFRSDVERELLDVLSNRDL
                     PDARRGLFHPDNFTFAQPVYLSSIYAAAHQVAGVESVDVRTFQRQGRPESSALDNGRM
                     DIGRLEIARLDNDRNFAEHGRLTISLGGGK"
     gene            1616..>2379
                     /locus_tag="PK224_21450"
     CDS             1616..>2379
                     /locus_tag="PK224_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18371.1"
                     /translation="MSQSPQNECGCCAGIAAETPALLFNRPGLSAIAYRVGTHQTFRR
                     SLQARLSNARWPVLRGLRTRDNDDFTIALLDAWAVTGDILTFYQERIANESYLRTATE
                     RLSILEQSRLLGYQLGPGLAAATHLAFTMEEAPGVPELAAQPTMLAIGTKVQSIPGPD
                     EQPHTFETIETIEARPAWNAFRAQLTETQTLAWDMTELWLAGANVTVTVGDLLLIVAP
                     NGSGYDASIRRVTEVESSREADRARVFLATISTSAG"
BASE COUNT          451 a          736 c          776 g          416 t
ORIGIN      
        1 aacgcgccga tacgatcttc gaagccatgg agccgctcca gtctctgtat gccgatcaca
       61 atgagctgtc gttttatacc tggagcaatc aacgttgttg cgtgcccaca ggtgcggtct
      121 cggccacgct cgcgggtcat catcctcacc tgacgccggg gagcatcctg ttgttcgaag
      181 aagtcctcgg tccggaaacg ggagcggcag ccgatgcgga tcctgcgcgg cgtcatgcgg
      241 tgaggctcga tcgtattgtc gcaacgacaa gcggcgggac gccgctgacc gatcctgtga
      301 ccgctcaact cgtcacggag atcacgtggc atgacgacga tgcgctgccg ttccccctct
      361 gcctctcagc cgcgaccagc gccgggtatc gagacggtgt cagcgtagct cgcggtaatc
      421 tgctgctggc cgaccatgga gtgacgctgg ccgaagaagc gctgggtgtg gtcccggagc
      481 cgttcttgtc catgccgcgc tcgaaggagg aagaccgttg tacgccctgt tctccccgga
      541 tgattccacc ccgtttcaga ccaggcttga ccaaggctcc cctgacacat gcgggccccg
      601 cttacgatca tgcaaagtct gcgtgggccg ctatgcagtg ggccctgcgc gatgtgcaac
      661 cggcgataac gctgacgggg accaaagaga gcgagacgac gacgtggacc gcacgacgag
      721 acctgctgaa tagtgacgcg gcggccgacg agtatgtggt tgagatcgag aacgacggtg
      781 gtggagcgat tcgtttcggt gacgatgtgc atggccggcg gccggaatcc ggcaccgact
      841 tcaccgcccg ctatcgggtc ggcaacggtc gcgccggcaa tatcggggcg gactcattgg
      901 tccatattgc gatcggcatt ccggaaatca cccgggtgcg gaatcccttg cccgccattg
      961 gcgggcagga accggaatcc ttgcaggatg tgcgtcagcg agccccggtg gcctatcgca
     1021 tccaggagcg agccgtgaca ccagtggatt atgccgaggt gaccgaacgc cgcgccgatg
     1081 tccaacgggc cgccgcgacg tttcgttgga ccggcagttg gcacaccgtc ttcgccacgg
     1141 tcgatcgcgg aggcggcgtg gcagtgaccg gcgaattcga ggcggacatc cgcgcgcata
     1201 tcgagcggta tcgcatggca ggccacgatg tggaaatcga tggaccgcag ttcgtggcac
     1261 tggagatcga gctgcatgtc tgtgtggcgc cggagcattt ccggagcgat gtcgagcgcg
     1321 aactgctcga tgtgctgagc aatcgggatc taccggacgc ccgccgcgga ctcttccatc
     1381 ccgacaactt taccttcgcg caaccggtct atctcagttc gatctatgcg gcggcccatc
     1441 aggtggcagg ggtcgagtcg gtggacgtcc gcacgtttca gcgacagggc cgaccggaaa
     1501 gttcggcgct ggacaatggc cgcatggaca tcggtcgcct ggaaatcgct cgcctggaca
     1561 acgaccgcaa ttttgcggag cacggccgac tgaccatcag cctgggaggg gggaaatgag
     1621 tcaatcgcca caaaacgaat gcggttgctg tgccgggatc gccgcggaaa ctcccgccct
     1681 gctcttcaac aggccaggcc tctcagcgat cgcttatcga gtcggaaccc atcagacgtt
     1741 tcgccggagc ctgcaggcca ggctctccaa cgcccgttgg ccggtgttac gtggattgcg
     1801 cacacgcgat aacgacgact tcacgatcgc gctgctggac gcctgggcgg tgaccggcga
     1861 catcctgacg ttctatcagg aacgaattgc caacgagtcc tatctccgca ccgcgacgga
     1921 acggctgtcc atcctggaac aatcccggtt gctcgggtat cagctcgggc caggactggc
     1981 cgcagcgacg cacctcgcct tcaccatgga ggaggcgccg ggcgtaccgg agctggcggc
     2041 tcagcccacg atgctggcga tcggaacgaa ggtgcaaagc attccggggc ccgatgagca
     2101 gccgcacacc tttgagacca tcgagaccat cgaggcgaga ccagcatgga acgcgtttcg
     2161 tgcgcagctg actgagacgc agacactcgc ctgggacatg acggagctgt ggctagccgg
     2221 cgcgaacgtg acggtgacgg tcggcgacct gttgctgatc gtcgccccga acggcagcgg
     2281 ctacgacgcg tcgatccgac gagtgaccga ggtggagtcc agccgcgaag cagaccgcgc
     2341 gcgggtcttc ctcgccacga tctcgaccag tgcagggag
//
LOCUS       DAORRQ010000188         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1050_length_2240_cov_21.902517, whole genome shotgun sequence.
ACCESSION   DAORRQ010000188 DAORRQ010000000
VERSION     DAORRQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_2240_cov_21.902517"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1509)
                     /locus_tag="PK224_21455"
     CDS             complement(343..1509)
                     /locus_tag="PK224_21455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRB18372.1"
                     /translation="MGSTIFVIDSSPAVHRMVEQISAPEGHKVMGFSDGPSALDAARK
                     LSPALVIADYHLDKITFSGLCKEIGRQDSLAETLIVSMVDGSDRLDESKLRSLGVRAF
                     LKKPLQREQLLDTIKGILNGAAGDPRGGKSAKTRTWPPVSTGTDDEDDESPNDASIDD
                     APHHEMEKEHSPMSPSLSPTAAPAASAPASNGPSGDALMKGLFDHLLHSVTVQADRKI
                     GELLPPAMAKEVAGQVSLAVGKAVQTEVAKQLADALAPERLQPVMRELILEELKRQAQ
                     TQLAGVEATVRRTVTELAPTLVEQSTEKRLGDLTDTGVKKHLPEALKSHLETIDQLVK
                     KEVEQVAANCARQAADEIVHEMAKDPIQQAVQRIVPDVAETQIRAEIKRLSSPD"
     gene            1731..>2240
                     /locus_tag="PK224_21460"
     CDS             1731..>2240
                     /locus_tag="PK224_21460"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRB18373.1"
                     /translation="MTRSRIQVVFFDAADTLFHIHGSVAEIYLQHAEKHGFRKTPESL
                     TAIKSAFARSFRDAPPPVFAATEPAAIKQSERLWWFDIVHNVFYRVGMFEAFDEFFED
                     VFARFEQPESWRLFPETLAVLTALKEQGFELGIISNFDSRLFSVLRGLGIADLFDTVT
                     ISSLAHAAKP"
BASE COUNT          415 a          650 c          684 g          491 t
ORIGIN      
        1 aaggccgacg caggcgtact ggcagtccgt cgaggaggcc gaacgagaac agccacgccg
       61 ggcgagagga tcggacgacg gagcaggcat tcatgaatag tccgggctgg aaggttgtag
      121 cggactcaag ccctgtgata tacatccggc ggttcagctg gttgttccgg atcgccggct
      181 tgagagccta tcaccgttga aggagagatt atgccgaggg gacgagagaa ggatcgcaag
      241 actcgcaaga agcatcggaa gaacgtggat cgtatgaagg ggctggtcaa ggcccgccgt
      301 gcggcggcca agaaggtcaa gcgagggtag gcgaacggga cgttagtcgg gcgagctcag
      361 tcgtttgatc tccgcccgga tctgagtttc tgccacgtct ggaacgatcc gctgtaccgc
      421 ctgctgaatc ggatcttttg ccatttcgtg cacaatctca tcggccgcct gtcgcgcgca
      481 gttcgcggcc acctgctcga cttccttttt caccagctgg tcgatcgttt caaggtgtga
      541 tttcaatgcc tccggcaggt gtttcttcac gccggtgtcc gtgaggtcac ccaatcgttt
      601 ctctgtggac tgctccacca gggtaggggc cagctcggtt acggtccgac gaacagtcgc
      661 ctcgacgccg gcaagctggg tctgagcctg tcgcttcaat tcttccagaa tcagttctcg
      721 catcacgggc tgtaagcgtt cgggtgcgag cgcgtctgcc agttgtttcg ccacttccgt
      781 ttgaacggcc ttccccacgg caagactcac ctgtccggcg acttccttcg ccatggcggg
      841 cgggagcagt tcgccgatct tccggtcggc ctggactgtg actgaatgca acaagtgatc
      901 gaacagcccc ttcatcaagg catctccgct cggtccgttc gaggcaggtg cagatgcggc
      961 cggggcggca gtggggctca gtgatgggga catggggctg tgctccttct ccatctcgtg
     1021 gtggggggcg tcgtcaatgg acgcatcgtt cggcgattcg tcgtcttcat cgtccgttcc
     1081 cgtggagaca gggggccacg tccgtgtttt tgcagatttc ccgccgcgcg gatctcccgc
     1141 cgcgccattc aagatgccct tgatcgtatc gagcagttgt tcccgttgca gcggtttctt
     1201 cagaaaggcc cggaccccga gcgaacgcaa tttgctttcg tccaggcgat cggagccgtc
     1261 caccatggag acgatcaggg tttcggccag actatcctgc cggccgattt ctttgcagag
     1321 tccggagaag gtgatcttgt cgagatgata gtccgcgatg accagcgccg gactgagttt
     1381 gcgggcagcg tcgagggccg aggggccgtc ggaaaatccc atgaccttgt ggccttccgg
     1441 cgcagaaatc tgttccacca tgcggtgcac ggccgggctg ctgtcgatca caaagatggt
     1501 cgatcccatc ccactcacct ctccataccc actcagccga agctaacaaa ggccccgtga
     1561 actgtcaaag aaaaggccga aagcagcggt ctgatgaggc ctctgcccac gcgccgcgag
     1621 agaggaactg aatgctggcg gagtcggtga ccatcctgta gaatccggcc gtcttcccgg
     1681 ctggcctatc ggccttcgtg gatggcgagc atgaggagtt acacgacaca atgacccgga
     1741 gccgaattca agtcgtcttt ttcgatgccg ccgacaccct gttccatatt cacgggtcag
     1801 tggccgagat ttacctgcag catgcggaga agcatgggtt caggaaaact ccggagtcgt
     1861 taaccgccat caaatccgcc tttgcccgtt cgttccgcga tgcgccgcca ccggtgtttg
     1921 ccgcgacgga acctgccgcg atcaagcagt ccgagcgatt gtggtggttc gacatcgtgc
     1981 acaacgtgtt ttaccgcgtc ggtatgttcg aggccttcga cgagtttttc gaagacgtgt
     2041 ttgcgcgctt cgaacagccg gagtcctggc gactgtttcc cgagaccctg gctgtgctca
     2101 cggctctgaa ggaacaggga ttcgagctgg gcatcatttc caacttcgat tcgcgattgt
     2161 tttccgtgct ccgtggcttg gggattgccg atctgttcga tacggtcacg atttcaagtt
     2221 tggcccatgc cgccaaaccc
//
LOCUS       DAORRQ010000189         2231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1054_length_2231_cov_3.506893, whole genome shotgun sequence.
ACCESSION   DAORRQ010000189 DAORRQ010000000
VERSION     DAORRQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1054_length_2231_cov_3.506893"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="PK224_21465"
     CDS             <1..1491
                     /locus_tag="PK224_21465"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRB18374.1"
                     /translation="GLTVVGNSFDATNGKKSDGGLVGLLLQDVSFPARIEENRVLGFV
                     FGIVLNNGLFTGVPFSLAAGSRIAGNYIVRLGAQVEAGTLKAFGIDVAADDCVICDNI
                     LLYASVEYGGIAVSGRNAVVERNQLRSILKEAGAVRPIALLLARLGGKGSLGTIGGRV
                     AGNTVLGVQEGIVVIGNEGAEVLDNRVESDGQEVGFGILLAASSRVRLQGNRVTNTAW
                     PIATSGGTANVVVDNRLVRGGGGVTVVFHTSLTCSQNRVEDMRHWGILSLVGFAKCAL
                     TENRVLNCGYQQVPSIGIGASLQIGELCVESCEVMNTGVSPDNTTVSSLSWGIVADLI
                     LEARVQSNLVTYANAALLDANQEHRALWMRGWWEQVITLGAGQVIFGFSAQILDNKFL
                     GPGRSALIEIAQQSVSDNLFRRFERLFFSNNFCWHASVAAQGTATVSLAGRSAIVMGN
                     HIKTNVPIPSVDFHGMKDAVYMGNLAQANPVNFGGIPSPISGFNKP"
     gene            1518..1838
                     /locus_tag="PK224_21470"
     CDS             1518..1838
                     /locus_tag="PK224_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18375.1"
                     /translation="MATLNQILAEQTKIIGEAKSSLEAAFKKPPTQAATIAAREATVA
                     DLKARVANLTETRAAVTRHIDQQVAGYQSEIVALEKLIEEDKKRFGDQPTPPRPVRGK
                     GRGK"
BASE COUNT          439 a          627 c          741 g          424 t
ORIGIN      
        1 ggcctgactg ttgtgggcaa cagcttcgat gccacgaacg ggaagaagag cgacggcgga
       61 ctcgtgggtc tgttgctgca ggatgtgtcc ttcccggcga gaatcgaaga gaatcgtgtg
      121 ttgggctttg tgttcggcat cgtgttgaac aacggtctgt ttaccggcgt gccgttttcg
      181 ctcgccgcgg gctcgaggat cgcgggtaac tacatcgtgc ggctcggtgc gcaagtggaa
      241 gccggtactc tgaaggcgtt cgggatcgac gtcgccgccg atgactgcgt catctgcgat
      301 aatattcttc tgtatgcctc cgtcgagtac ggggggattg cggtgagcgg caggaatgcc
      361 gttgtcgagc gcaaccaact ccgttcgata ctgaaggaag cgggggctgt gcggcctatt
      421 gctctcctgc tcgcccggct tggcgggaag gggagtctcg ggacgatcgg cggccgggtc
      481 gccggcaaca cggtcctcgg cgtgcaggaa gggatcgtcg tgatcggcaa cgaaggggcc
      541 gaggtactgg acaaccgggt ggaaagtgac gggcaggagg tcgggttcgg tatcttgctc
      601 gcggcatcga gtcgtgtccg cctgcaaggc aatcgtgtca ccaacacggc ctggcccatc
      661 gcgaccagcg gcggcacggc gaatgtcgtc gtcgataacc gtctcgtacg cggtggcggc
      721 ggcgtcacgg tggtcttcca cacgtccctg acctgcagcc agaatcgcgt cgaagacatg
      781 cgacattggg gcatccttag cctggtcgga tttgcgaaat gcgccttgac cgaaaaccgc
      841 gtgctcaatt gcggctatca gcaagtgccg tcgatcggca tcggggcttc cttgcaaatc
      901 ggagagttgt gcgtggaatc gtgcgaggtg atgaacaccg gagtctcacc cgataatacg
      961 acggtgagtt cgctgtcctg gggaatcgtc gcagacctga ttctggaggc ccgggtgcag
     1021 agcaatctgg tcacctatgc caacgcggcg ctgttggatg cgaatcagga acatcgcgcg
     1081 ctctggatgc gtggctggtg ggaacaggtg atcaccctcg gcgcggggca ggtgattttc
     1141 ggcttcagtg ctcagattct ggataacaag tttctcggac caggccggtc agcgttgatc
     1201 gaaatcgccc aacaatccgt gagcgacaat ctgtttcggc ggttcgaacg gcttttcttc
     1261 agcaataatt tttgctggca tgccagcgtc gctgcgcagg ggacggctac ggtgtctctc
     1321 gcgggacgga gcgccatcgt catgggcaac catatcaaga cgaatgtgcc gatcccctcc
     1381 gtggattttc acgggatgaa agacgcggtg tatatgggca acttggccca ggccaacccg
     1441 gtgaatttcg gcggcattcc atccccgata tccggtttca ataaacccta gcagtccgtg
     1501 ggtaaaggag catagagatg gcgaccttga atcagatcct ggcagagcag acaaaaatca
     1561 tcggtgaagc gaagtcgtcc ctggaggctg cattcaagaa gccacctacc caggcggcca
     1621 cgatcgccgc tcgggaagcg acggtggcag atttgaaggc aagggtggcc aatctcacgg
     1681 aaaccagggc tgccgtcacc agacacatcg atcaacaggt ggccggatat cagtctgaaa
     1741 tcgtggcctt ggagaaactt atcgaagagg acaaaaaacg gttcggcgat cagccgacgc
     1801 ctccgcggcc ggtgcgcgga aaggggcgag ggaagtaaag gctgttcccg tccctttctg
     1861 gaacggttat ggagcgggtc atgcaggcca gagcgggatc gcctcagcag gaggggacgc
     1921 tggggtcctg ctggacagga ccgtcgatgg gccgtccggt cggaggccgt gatgggcaac
     1981 ccgaggaggg acaggccgcc tcccgcccac cccggatcga acaccatatc ggccgatgtg
     2041 cgatctttcc gcccgcaggg actcacgggg gaaaagcaga acccgttccc taccgagcca
     2101 gcatggagag ggccttccag gccgacctct ctgacgttcg agcctttcgt ggcgcatccg
     2161 ttttgcgggg ccgggcgccc ggccccgccc ggcaaacccg atgccccccc cacgcgcccc
     2221 cgggacagac g
//
LOCUS       DAORRQ010000190         2192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1066_length_2192_cov_10.179691, whole genome shotgun sequence.
ACCESSION   DAORRQ010000190 DAORRQ010000000
VERSION     DAORRQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1066_length_2192_cov_10.179691"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PK224_21475"
     CDS             <1..894
                     /locus_tag="PK224_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRB18376.1"
                     /translation="NTEIDTGLQGKGRGCWMAFGYVLARGQSNVIALHDCDILSYNRE
                     YLARLCYPIVNTNLGYEFCKGYYSRVTNRLHGRVTRLYLTPLIRSLQQVAGSHPLLTF
                     LDSFRYPLAGEFAMVRDLAWINRVPGDWGLEVGVLSEIYRNCALRRICQADIADAYEH
                     KHQSLSADNAEQGLQKMCVDITKSLFRNLASEGVVLSEAVLKTLRATYLQTAQEAITR
                     YQNDAAINSLQFDRHEERMAVEVFLKGMKIATEAFLDDPLGVPMISNWSRVTGAIPDI
                     FERLIGAVERDHEWDPVGDRV"
     gene            1293..1637
                     /locus_tag="PK224_21480"
     CDS             1293..1637
                     /locus_tag="PK224_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18377.1"
                     /translation="MTDDYAQRKVQVIRDFLAAGYPAPYTVEAVIPRRDPLADTKFKI
                     ILNNQVERTLAVCAAVVLDSLPTPDQLKQLLVSQGILGQVKASTDSRICHEALGTDEE
                     SHVRPMRSVRGT"
     gene            complement(1800..>2192)
                     /locus_tag="PK224_21485"
     CDS             complement(1800..>2192)
                     /locus_tag="PK224_21485"
                     /inference="COORDINATES: protein motif:HMM:NF019544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18378.1"
                     /translation="RSHHIPPYTGVCEIGGRSPPDNVFDNAMDRTEQVVDLLERTKEY
                     GILLVGVFCSLEELNRRERQRQDRTIGEAAAQINLVYRAFSYDVEVDSSTKAPSELAL
                     EIMDYIKRVDVLHGRDKTMAKIQTLSRL"
BASE COUNT          500 a          628 c          602 g          462 t
ORIGIN      
        1 aacactgaaa tcgacaccgg gctgcaaggc aagggccggg gctgttggat ggcattcggc
       61 tacgtcttgg cccgcggcca aagcaacgtg atcgcgctcc acgactgcga cattctcagc
      121 tacaaccggg aatatctcgc gcgactctgt tatccgatcg tcaacaccaa cctcggctac
      181 gagttctgca agggctatta cagccgggtc acaaatcgcc tccacggccg tgtcaccagg
      241 ctgtatctca cgccgctgat ccgaagcctc caacaggtgg ccggatcgca tccgttgctg
      301 accttcctgg atagttttcg ttatcccctg gccggtgagt tcgccatggt gcgggatctt
      361 gcctggatca accgggtacc cggcgactgg ggactggaag tcggagtcct ctccgagatc
      421 tatcgcaact gcgcgctcag gcggatctgc caggccgata ttgccgacgc ctacgaacac
      481 aaacaccaga gcctgtcggc cgacaacgcc gagcaagggc tgcagaaaat gtgcgtggat
      541 atcaccaagt ctctcttcag aaacctcgcg agtgaaggcg tggtgctgtc ggaagccgtg
      601 ttgaaaaccc tacgggccac gtatctacag accgcgcagg aagcgatcac ccgctatcag
      661 aatgatgcgg ccatcaacag cctgcagttc gaccggcatg aagagcgaat ggccgtcgaa
      721 gtctttctga agggcatgaa gatcgccacg gaagcgtttc tggacgatcc gctcggggtg
      781 ccgatgatct ctaattggag ccgggtcacc ggcgccattc ccgatatttt cgaacgcttg
      841 atcggcgcgg tggaacgcga ccatgagtgg gatcccgtcg gggaccgggt gtaacgggtg
      901 acagaacacc cacggagatc tcgatgcgac tgacagatgg atcctggatc tgcaccaagt
      961 gcagagtgct gtgctcgctc aaaggatatg aagagggtcc gttggcctca atgcgggaaa
     1021 tttgcagcaa ttgcaaagag cgcgctgaag caagctggct gactgcgata ggcgatagac
     1081 gacgactgcc ccaaccctcc tagagcgatc gtcccggtct catgccaatc cggccacagt
     1141 cctcgataga ttccgctcct ggaacatcca cacatcatag cctctgaacg tcttgccgcc
     1201 ttccagtcga ttggcgtagg attccggcac aggttgaatc acaaaacgcc ggcgaaagaa
     1261 gcattggcga ggaaacagaa ggacgagcaa cgatgacaga cgattatgct cagaggaagg
     1321 tgcaagtcat ccgggatttt ctggctgccg gatatccagc cccctacacc gtggaagccg
     1381 taattcctcg acgagatccg ttggccgaca cgaagttcaa gatcattctg aacaaccagg
     1441 tggaacggac cctcgccgtc tgcgccgccg tcgtgctgga cagccttccc acacccgatc
     1501 aactcaagca actgctggtc tcgcagggca ttctgggcca ggtcaaagca tcgacggatt
     1561 cccggatttg ccatgaggcc ttgggaaccg acgaagaatc acatgtgcgc ccgatgaggt
     1621 cggttcgggg gacgtagttc tacctcgcgt cgtctcgaat cattcgttca tcggcagaat
     1681 caatgactgc ttgcacgcag aacacgactc agcagttcga attgccttac ctcgttctca
     1741 ggaatagagg cctggtgatc aagtccttct tcagttcgtg aactgccatg cgtacagttc
     1801 taaaggcggc tgagtgtttg tatttttgcc atggtcttgt cgcgtccgtg taacacatca
     1861 acgcgcttga tgtaatccat gatctccaag gccagctcag atggcgcttt ggtgcttgaa
     1921 tcgacctcaa catcgtaact gaaagcccga tatacgagat tgatctgagc agccgcttcc
     1981 ccgatcgttc gatcctgccg ctgccgttcc ctccgattca gttcttccag tgaacagaaa
     2041 acgcccacca agagaatgcc atattcttta gttctctcca ggaggtcgac aacttgttcg
     2101 gttcggtcca tcgcattgtc gaagacgttg tcaggaggtg accttccccc gatttcacag
     2161 acacctgtat aaggaggaat gtgatgagat cg
//
LOCUS       DAORRQ010000191         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1071_length_2174_cov_5.871166, whole genome shotgun sequence.
ACCESSION   DAORRQ010000191 DAORRQ010000000
VERSION     DAORRQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1071_length_2174_cov_5.871166"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..>360)
                     /locus_tag="PK224_21490"
     CDS             complement(156..>360)
                     /locus_tag="PK224_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18379.1"
                     /translation="TGLDDMTFCRRLLDDHHTAVTPGRSFGEAYASFIRIAVCGQEED
                     VHEGLSRTIAFAQQLGGCRVQAA"
     assembly_gap    361..370
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<371..1276)
                     /locus_tag="PK224_21495"
     CDS             complement(<371..1276)
                     /locus_tag="PK224_21495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRB18380.1"
                     /translation="MDRAQALERQGYRIYHLELGNPRMAPPGEIVDGTVQALREKQLG
                     YAPMAGVKELRAALAARYTSLTGEALTDSQVVISPANLLIHQFLDITCNPGDRVVLFT
                     PAFPSYWAAATHQGLQVRPVPLSERDGFHLTRQAIDDALAAGPRAIVVNNANNPTGAL
                     YDSTILRALAARCEEAGIWLLSDETYADLTFDGSFVTLASARAGYVVAMSSFSKVCSI
                     PGFRTGYAFAHEAVAAKLALSNSTLYSCLPLFTQLGCLAGMKVLDTYANEVRSRGSRL
                     IGSCHDRINRSGILHCHKPESGFYLF"
     gene            complement(1372..>2174)
                     /locus_tag="PK224_21500"
     CDS             complement(1372..>2174)
                     /locus_tag="PK224_21500"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRB18381.1"
                     /translation="AAVRDGENNPLPGRRQFADCDVVILCGGLGTRLQSVVADRPKSM
                     TEIHGRPFVACLVEHFLRHGTRRFIFSTGYKGDMIEDWFGRHRGGYETLFVRDPAPLG
                     TGGAVAQAMKLVRSNPFLVLNGDSLCELDPERLLRFHTRKSARATIAVTQADSREDTG
                     AVTLGEDDRVLSMVEKPRTRTTGYHNAGIYLFDRTVEALFPNSRSWSLERELLPQLVT
                     EPFYGFVTASALYDIGTPERLAHFREVWKDPSIHFPVTSMHQEQGSLL"
BASE COUNT          460 a          651 c          651 g          402 t
ORIGIN      
        1 cctctgcctc ggcatccaga atggccacgg agcagctgtg ttgtcgcagg aaggtcgcga
       61 tcaaaccaga ccagaccggc ggttcgatgg cggcgaagtc ccggctcaac tcctgataca
      121 cctgtacccg actaggcggg gtgatcagca acacgtcaag cggcttggac acggcaacct
      181 cccagttgtt gcgcaaaggc gatcgtacgc gacaggcctt cgtgcacatc ctcttcctgc
      241 ccgcacacgg ctatgcgaat gaacgacgcg taggcttcgc cgaaactacg gcctggggtg
      301 accgccgtat ggtgatcgtc cagtagtcgc cggcaaaagg tcatatcgtc gagcccggtg
      361 nnnnnnnnnn aaacagataa aaccctgact ccggcttgtg gcaatgcagg atgccggagc
      421 ggttgatgcg gtcgtggcag gatccgatga ggcgggagcc gcgtgatcga acctcgttcg
      481 cataggtatc gagtaccttc ataccggcca agcaacccag ttgagtaaac aggggaagac
      541 aggaatagag ggtggaattg gagagcgcga gttttgccgc caccgcctca tgggcgaagg
      601 catatccggt tcgaaaccct gggatcgagc acacctttga aaaggacgac atcgctacga
      661 cgtatccggc tcgcgccgag gcgagggtca cgaacgaacc atcgaaggtc agatcggcgt
      721 acgtttcatc gcttaacagc cagatccccg cctcttcaca gcgtgccgca agcgcgcgca
      781 gaatcgtcga atcgtagagg gcccccgtgg ggttgttggc gttgttgacg acaatcgccc
      841 ttggtccggc agccagcgcg tcatcgatgg cctgccgggt caggtgaaat ccgtcgcgct
      901 cagaaagcgg caccggcctg acctgaagtc cctgatgcgt cgcagcggcc cagtaggagg
      961 gaaatgccgg cgtgaacagc accacgcgat cgccgggatt gcaagtgatg tccagaaact
     1021 gatgaatcag gagattcgca gggctgatca cgacctggga gtcggtcagc gcctcgcccg
     1081 tcaacgaggt gtatcgagcc gccaacgcgg ctcgtaattc cttcacgccg gccatcggcg
     1141 catatcccag ctgtttctcg cggagcgcct gcacggtgcc gtcgacgatt tcacccggcg
     1201 gagccatgcg gggattgccg agttccagat gatagatgcg atacccctgc cgctccaggg
     1261 cttgagcccg atccatgact cggaacatct cctgcccgat caggcgttca cctcgcgatg
     1321 aaaatgccaa tcggcgcgtt gtctgtgaac ccgggactgt ccgatgagcc attacaacaa
     1381 cgatccttgt tcctgatgca tggatgtgac cggaaaatga attgacgggt ctttccagac
     1441 ctcgcgaaaa tgcgcgaggc gctcgggggt gccgatgtcg tacagcgcgc tggctgtgac
     1501 gaatccatag aacggttcgg tgaccagctg cggaagtaac tcgcgctcca gtgaccatga
     1561 acgactgttc ggaaacagcg cctcgacggt acgatcgaac agatagatgc cggcattgtg
     1621 atagcccgtc gttctggtgc gcggcttctc caccatggac aggacgcggt cgtcctcccc
     1681 taacgtgacg gctccggtat cttcccgtga gtcggcttgg gtgaccgcga tcgtcgcgcg
     1741 ggcgctttta cgggtatgga accgcaggag tcgctccgga tcgagctcac agagggagtc
     1801 tccgttcagc accaaaaatg gattgctgcg aacgagcttc atcgcttgcg cgaccgctcc
     1861 gcccgttccc agcggagcgg ggtcgcggac gaacagggtt tcatacccgc ctcgatggcg
     1921 gccaaaccag tcctcgatca tgtcgccctt gtatccggtg ctgaaaataa accgacgcgt
     1981 gccatggcgc agaaaatgtt cgacgagaca tgccacaaac gggcggccat gaatctccgt
     2041 catcgatttc gggcgatccg ccacgacaga ttgcaggcgt gtaccgagcc ccccgcacag
     2101 aatgaccaca tcgcaatccg caaactgtct tctccccggc agcggattat tttccccgtc
     2161 acgaacagcg gcta
//
LOCUS       DAORRQ010000192         2145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1080_length_2145_cov_33.654067, whole genome shotgun sequence.
ACCESSION   DAORRQ010000192 DAORRQ010000000
VERSION     DAORRQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1080_length_2145_cov_33.654067"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK224_21505"
     CDS             <1..761
                     /locus_tag="PK224_21505"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRB18382.1"
                     /translation="PPGWALGRPRDHPGPGRGRGPPGSGATGPIQASPSSLALTSANA
                     VGTLSLRKTGTDQHVYSLSTSQGWVWLNPPYGSTQTISTETDQIVVTAKPTGLAAGTY
                     SAVVYIVESGPNNFSNTLRIPVTFTIAAGQTAAAAPSTPTQSSVTTPPPPPPSPTAPL
                     PVATTPSSTAPKTASATVSWNANTESDLAGYRIYVGTRSGSYGFAGPFEVTNRTNFTI
                     QNLPVGATYFFSVSAFDKSGNESAKSAEVSKSLF"
     gene            complement(821..1657)
                     /locus_tag="PK224_21510"
     CDS             complement(821..1657)
                     /locus_tag="PK224_21510"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRB18383.1"
                     /translation="MRLLVSLLILSLSIAGLATGDSAYAQLERLRKNKEVEAAPLPDP
                     PMVSIPEGDFLMGADGTDALEDERPQHRVWVDRFEIDRYEVTTRRYAEFLSVTTRAEP
                     WQWEAVDPARHHDRPVIGVSWFDAEAYCRWRSARLPTEAEWEKAARGSDGQLFPWGNR
                     TPTDEVANFGLGARFSYSQVLAPVQGYQPGRSPYGLHQMAGNVGEWVADWYGANYYET
                     SASKNPAGPERGAFRVVRGGSWSDLPKYLLTYGRSKLPPETRNSYTGFRCARAVLPIT
                     SP"
     gene            complement(1787..>2145)
                     /locus_tag="PK224_21515"
     CDS             complement(1787..>2145)
                     /locus_tag="PK224_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18384.1"
                     /translation="RFQSLDLPEQADALLSNSLLHHVPNPLQFWFRLKQLAKPGACVL
                     VMDLLRPDSPEAAQALVDQYAGDEDPILKRDFYRSLLAAFTEDDVAAQLAEMNLSRLL
                     IDVPDDRHWVVGGILL"
BASE COUNT          455 a          697 c          618 g          375 t
ORIGIN      
        1 cccccccggg gtgggcgctt gggcggcccc gcgaccaccc cgggccgggg cggggccggg
       61 ggccaccggg gtctggggcc accgggccga tccaggccag tccgtcttcg ctggcactca
      121 ctagcgccaa cgcagtcggc accttgagcc tgcgcaaaac aggaacagac cagcatgtct
      181 attcgctcag caccagccag ggctgggtgt ggctcaatcc accgtacggg agtactcaga
      241 cgatctccac tgaaacagat cagattgtcg tgacggccaa gcccaccggt ctcgcggcag
      301 gaacctattc agcggtggtg tatatcgtcg agtccggccc gaataatttc tccaacacgc
      361 tgcgcattcc ggtgaccttc acgattgctg ccggccagac ggctgctgca gcaccgtcga
      421 ctccgacgca atcgtcggtc accacgccgc ctcctccgcc ccccagtccg acggcaccgc
      481 ttccggtggc gaccacgcct tcgtcgacgg cacccaagac tgccagtgcc acggtcagct
      541 ggaatgcgaa taccgagtca gatctggcag ggtatcggat ctacgtcggg acgcgatcgg
      601 gcagttatgg atttgcgggg cccttcgaag tcaccaaccg caccaacttc acgatccaga
      661 atctaccggt cggtgcgacc tatttctttt ccgtatcggc cttcgacaag tctgggaacg
      721 aaagtgccaa gtctgcggag gtcagcaaga gcctgttcta agcgaacgcg aaatagagaa
      781 gagcgcgaga ccgtgagctg gcgcgcagca ggcgcccagc ttacggggaa gtgatcggaa
      841 ggaccgccct ggcgcagcga aatcccgtat agctattgcg agtctccggc ggcaacttcg
      901 accgaccgta ggtcagcagg tacttcggca agtccgacca ggacccgcca cgcacgacac
      961 gaaacgctcc ccgttccggt ccggctggat tcttcgaggc gctggtttcg tagtaattgg
     1021 ccccgtacca atcggcaacc cattccccga cattccctgc catctgatgc agcccatagg
     1081 ggctgcgacc gggctgatat ccctgaacgg gcgcgaggac ctgactgtaa ctgaatctgg
     1141 cgccaagccc gaaattcgcc acctcatcag tcggagtccg attgccccag gggaacaatt
     1201 gtccatcgct cccccttgcg gccttctccc actcggcttc agtcgggagc cgtgcactcc
     1261 gccaacgaca ataggcctcc gcatcgaacc aactaacgcc gatgaccggg cgatcatgat
     1321 gccgggccgg atccaccgcc tcccattgcc agggctcagc ccgggtcgtc accgagagaa
     1381 attcggcata cctccgggtt gtcacctcgt accggtcgat ctcgaaccgg tcgacccata
     1441 cccggtgttg cggccgctca tcctccagcg catccgttcc gtcggccccc atgagaaagt
     1501 ctccttcagg aatcgagacc atgggcgggt cgggaagcgg cgcagcttca acctctttgt
     1561 ttttccgcag ccgctcaagc tgtgcgtacg ctgaatcgcc ggttgccaaa ccggcaatcg
     1621 agagagacag gatgaggaga ctcacaagca atcgcatgcc gggaagcata cacaaacagc
     1681 ccccccaggc gcgagcgctc tgcgagaacc ctgaacgagt catgagaaga gcagggtggt
     1741 caaaacgagg tgagaacgcc gttggagctc gttttcacca tgctgattaa agaaggattc
     1801 caccgacgac ccaatgccgg tcatccggca cgtcgatgag gagccgggag aggttcatct
     1861 cggccaattg cgccgcgaca tcatcctcgg taaacgcggc cagcaacgag cgatagaagt
     1921 cgcgtttcag gatcggatcc tcgtcacctg catattgatc cactaaggcc tgggccgctt
     1981 caggagagtc cggccgcaaa agatccatca ccaggacaca agcgccgggc ttagctaatt
     2041 gcttcagacg aaaccagaac tgtagcggat tcggaacatg gtgcagcaga ctgttggaca
     2101 gcagtgcatc ggcctgttcc ggcaagtcga gcgattgaaa ccgct
//
LOCUS       DAORRQ010000193         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1096_length_2101_cov_6.063539, whole genome shotgun sequence.
ACCESSION   DAORRQ010000193 DAORRQ010000000
VERSION     DAORRQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_2101_cov_6.063539"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..280)
                     /locus_tag="PK224_21520"
     CDS             complement(161..280)
                     /locus_tag="PK224_21520"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18385.1"
                     /translation="MCLDHPVFSVQYHPEAAPGPHDSAYLFEQFVALMETHYA"
     gene            complement(237..1322)
                     /gene="carA"
                     /locus_tag="PK224_21525"
     CDS             complement(237..1322)
                     /gene="carA"
                     /locus_tag="PK224_21525"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRB18386.1"
                     /translation="MKKAILALADGTWFEGRALGAEGETGGEVVFNTAMTGYQEVLTD
                     PSYRGQIVTMTCPHIGNYGVTPEDIESNRIWAEGFVVKESSRLASNWRSKATLQEYLQ
                     AANIVAIEGIDTRALTTHLREKGAQPGVISHIDLDPRRLADKARKAPSIIGRDLAATV
                     TCERRYTWTAGTGDWAPKLTMPEPGAAQAARKTWRVVAYDFGVKQNILRRLVDVGCEV
                     TVVPASTPAKDVLALNPQGLFLSNGPGDPEGVPYAMDALRELIGRLPIFGICLGHQLL
                     GLALGFSTYKLKFGHHGANHPVIDLRTRKVEITSLPDRGTGARTGHADCGHAVRARAV
                     DPYQSQRWIGGRDDVSGPPGFLGPVSP"
     gene            complement(1332..1835)
                     /locus_tag="PK224_21530"
     CDS             complement(1332..1835)
                     /locus_tag="PK224_21530"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HRB18387.1"
                     /translation="MRQGLIACITCELLALAVWIGGLLVLVAAVIPAVFNTFGGQDTG
                     GFFLTRAFDGYNRLVLGSAAILTSGVLWRAWLVQRGLADGEITRTEWLLLGAMLLTAG
                     VITFVLHPQAAALQAQAFASKGEEARKAAFEAFFQLHKPVRALYIVNVGLGIALLTVR
                     VRSWVPR"
     gene            complement(1848..>2101)
                     /locus_tag="PK224_21535"
     CDS             complement(1848..>2101)
                     /locus_tag="PK224_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRB18388.1"
                     /translation="SAVDKLATAPAKAFSLNAGTLAVGAPADVAIVDPNREWQVDPSR
                     FRSKSRNTPFAGWKVKGRVTTTIVSGRVVFELDRSERQA"
BASE COUNT          392 a          655 c          652 g          402 t
ORIGIN      
        1 ctgcatcagg gccaggacaa ccacggcaac cgcggcacag agatgccaag ggctgagggc
       61 ctgtccgact ttgcggttca tgtcgaggcg acggagatgc ggcctcgcat ttggctgtag
      121 atcgttcgcc acagcaggca ggtcaacgtc gggcaggctc ctatgcataa tgtgtctcca
      181 tcaacgcgac aaattgttcg aagagatagg ccgagtcgtg cgggcccggt gccgcttcag
      241 ggtgatactg gaccgagaaa accgggtggt ccagacacat catcccttcc accgatccat
      301 cgttgagact ggtatgggtc aactgcacgc gcccgaacgg cgtgtccaca atcggcatgg
      361 cctgtccttg cgccggtgcc ccgatcggga agcgaggtga tctcgacctt ccttgtccgg
      421 aggtcgatga ccgggtgatt ggcgccatga tggccgaact tcagtttgta ggtggagaag
      481 ccaagcgcca ggccgagcaa ttggtggcct aaacagatgc cgaaaatcgg taaccggccg
      541 atcagttccc gaagcgcgtc catggcatag ggcaccccct ccgggtcccc tgggccgttc
      601 gagaggaaca atccctgagg attgagcgcc agcacgtcct tcgccggggt ggatgcgggc
      661 accacggtga cctcgcagcc gacatcgacg agccgccgga gaatgttctg tttgacgccg
      721 aaatcatagg ccaccacgcg ccaggttttt cgggctgcct gcgcggcgcc aggctcgggc
      781 atcgtcagct tcggcgccca gtcgccggtc ccggctgtcc aggtgtagcg tcgctcgcag
      841 gtcacagtgg cggccagatc ccgtccgatg atgctcggag ccttccgtgc cttgtcggcg
      901 aggcgacgcg gatcgagatc gatgtgggaa atcacgcccg gctgggcgcc cttctcccgc
      961 agatgtgtgg tgagggctct ggtatcgatg ccctcgatcg ccacgatgtt cgcggcctgc
     1021 aaatattcct gaagcgtagc cttgctacgc cagttgctcg ccaggcggct ggactccttg
     1081 acgacgaatc cttccgccca gatccgattg gactcgatgt cttccggtgt cacgccgtag
     1141 ttgccgatgt gaggacaggt catggtcacg atctgtccac ggtaggaggg atcggtcagc
     1201 acttcctgat agccggtcat ggccgtgttg aacacgactt cgcctccggt ctccccttct
     1261 gcgccgaggg ctcgtccttc gaaccaggtg ccgtcggcga gtgcaagaat cgctttcttc
     1321 aagatgactc cttatcgagg gacccaggat cgtacgcgca cggtcagcag cgcgattccg
     1381 agtccgacat ttacgatata caaggcccgt accggtttgt gcagttgaaa gaatgcttcg
     1441 aacgccgctt tccgcgcttc ctctcctttg gaggcaaagg cctgtgcctg cagggccgcc
     1501 gcctggggat gtagcacaaa cgtgatgact cccgcggtga ggagcatggc tccgagcaac
     1561 agccattcgg ttctggtgat ctcgccatcc gccaagcccc gctggaccag ccaggcccgc
     1621 cacaggactc ccgaggtcag gattgccgcc gatccgagca ccagacggtt gtacccgtcg
     1681 aaggcgcggg tcagaaagaa tccgccggtg tcctgcccgc cgaacgtatt gaacacggcc
     1741 gggatgacgg cggcaacgag caccaagagc ccaccgatcc agaccgccag ggccagcagc
     1801 tcacaggtaa tacaggcgat cagtccttgg cgcaccgtgt cgaatcccta tgcctgtcgc
     1861 tctgagcgat cgagttcgaa cacgacacga ccggagacga tggtggtcgt cacccgtccc
     1921 ttcaccttcc agccggcgaa tggtgtgttg cggctcttgg aacgaaaccg tgacgggtcc
     1981 acttgccact ccctgttcgg atcgacgatg gcgacgtcgg ccggcgcgcc gaccgccagg
     2041 gttccggcat tgagactgaa cgctttggcc ggcgcggtcg caagtttatc taccgcggat
     2101 t
//
LOCUS       DAORRQ010000194         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1099_length_2088_cov_7.030497, whole genome shotgun sequence.
ACCESSION   DAORRQ010000194 DAORRQ010000000
VERSION     DAORRQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_2088_cov_7.030497"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PK224_21540"
     CDS             complement(<1..824)
                     /locus_tag="PK224_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB18389.1"
                     /translation="MSDRVAYDTWVQANQRCLVGEFDRLKARLTGGESAETAGHNIDE
                     IDAEGPAPAAIDVLTNLFGLSRFERDVLLLCAGVEMNSELARVCGEALTPGHRSFVTF
                     GLALAALEAPHWSALTPVGPLRRWRLVELDESPGVANARLRIDERVLHYLAGVNYLDP
                     RLRPLLRIRQPGELLAVAHRQTAATILSAIEAGRSSSGLVLLTGDDLQGQGDVAASVA
                     SELGLQLYMLPAALVPPSASEIEALAVLWQREAFLLHAALLVECAEHEVPKQAGSF"
     gene            complement(821..2086)
                     /locus_tag="PK224_21545"
     CDS             complement(821..2086)
                     /locus_tag="PK224_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HRB18390.1"
                     /translation="MLRDLLNDGLINHNVSGLLGSTVTVSAVPPDRVVPANGAESTQL
                     NLFLHQVTFNTGWRNHALPSRDGSGTQRLSNPPLALDLHYLVSAYSAEELGSEILLGY
                     AMQLLHETPVLDRKAITTALNPSPSVETTLPPALRALAECGLADQVEQIKLVPDPLST
                     EEMSKLWTAVQSHYRPTAAYVATVVLIESSKPTKPTLPVLTRGPVDPVTKLERGVVVQ
                     ADLLPPFPTIQTVQAIDGQPVAAVGATVELTGHHLNGTNRTVVFSNARFDIVHEVAAL
                     AGDSTSQLQVVVPPSLAVGFYRVAVRLVRPTETEPRISNQLGLIVGPLITTALPLTVP
                     RDGSGRVTITLGCAPDVRAGQSVSLLLGSREVLPESWSGPADSFTFILEQAPVGDHLA
                     RLRVDGIDSPLIDRAAEPPVFFNYRVTIT"
BASE COUNT          415 a          673 c          657 g          343 t
ORIGIN      
        1 atgaagctac ctgcctgttt cggcacctca tgctccgcgc actccacaag caacgccgca
       61 tggagcagaa acgcctcccg ctgccataac accgccagcg cctcgatctc cgacgcgctg
      121 ggcggcacga gcgcggcagg aagcatatac aactgcagac ccagttccga tgccacggaa
      181 gccgccacat cgccctgccc ttgcagatcg tcgcccgtca gaagaacgag gcccgacgag
      241 gatcggccgg cttcgattgc ggagaggatc gtggccgcgg tctgtcgatg ggcgacggcc
      301 aacaactcgc ctggttgtct gatacgcagg agaggccgca accgtggatc gagataatta
      361 acgcctgcca gatagtgcaa cacgcgctcg tcgatacgga ggcgcgcatt cgccactccc
      421 ggactctcat cgagttccac gagccgccac cggcgcaacg gtcccaccgg cgtcaacgcg
      481 ctccaatgcg gcgcttcgag tgcggccagg gccaagccga aggtgacgaa cgaccgatgg
      541 ccgggagtca gggcctcgcc acagacacgc gctaactccg aattcatctc gacgcctgcg
      601 cagagcaaca gcacatcgcg ttcgaagcgg ctgaggccga acaggttggt gaggacatca
      661 atggcggccg gggcgggtcc ttccgcatcg atctcgtcga tattgtggcc ggcggtctcc
      721 gcagactccc caccggtgag cctggccttc aggcggtcga actcaccgac caaacaacgt
      781 tggttggcct ggacccaagt gtcgtacgcg acgcggtcgc tcatgtgatt gtcacccgat
      841 aattgaagaa caccggcggc tcggccgcac gatcgatcaa cggactgtcg atgccatcca
      901 cgcgcaaccg ggccaaatga tcaccgaccg gcgcctgttc gaggatgaac gtaaaggagt
      961 ccgccgggcc ggaccacgat tcaggcaaga cctctcggct tccgagcaag agggagaccg
     1021 attgtccggc tcgaacgtcc ggcgcacagc ccagcgtgat ggtcaccctg ccgcttccat
     1081 ccctgggcac cgtcagggga agcgcggtcg tgatgagagg gccgacaatc agccctagct
     1141 ggttgctgat ccggggctcg gtctccgtcg gacgaacgag tcgcacagcc acccggtaga
     1201 aaccgacggc caacgacggc ggcacgacca cctggagctg cgaggtcgag tcgccggcca
     1261 aggccgccac ctcgtgcacg atgtcgaatc gagcattcga aaacaccacg gtgcgattgg
     1321 taccgttgag gtgatgtccg gtcagttcga cggtcgcccc cacggcggcg accggttgcc
     1381 catcgatcgc ctgcactgtc tggatggtcg gaaacggggg caggagatcg gcctggacca
     1441 ccacgccccg ctcgagtttc gtcaccggat cgaccggccc gcgggtgagc accggcaatg
     1501 tcggcttcgt cggcttcgag gactcgatca acaccaccgt cgccacatag gccgcggtgg
     1561 gtcggtaatg ggactgcacg gcggtccaga gtttggacat ctcttcggtg ctgaggggat
     1621 ccggaaccag cttgatctgt tccacttgat ccgccagccc gcattcggcc agggcccgca
     1681 aggccggcgg caacgtcgtc tccaccgacg gagaaggatt cagcgcggtc gtgatggcct
     1741 tccgatctaa gaccggggtc tcatggagca actgcatggc ataacccaac aggatctcgc
     1801 tacccaattc ctcggcgctg taggcgctca cgaggtaatg aagatccagg gccaacggcg
     1861 gattgctgag ccgttgcgtg cccgatccgt cgcgggacgg caaggcatga ttccgccagc
     1921 ccgtattgaa tgtgacttga tggagaaata gattcagttg ggtgctttca gcgccattgg
     1981 cggggaccac ccgatccggc gggacggcgc tcaccgtgac cgtactgccg agcaggccgc
     2041 tgacgttgtg gttgatcaag ccgtcgttga ggaggtctcg aagcacgg
//
LOCUS       DAORRQ010000195         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1113_length_2056_cov_6.379810, whole genome shotgun sequence.
ACCESSION   DAORRQ010000195 DAORRQ010000000
VERSION     DAORRQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_2056_cov_6.379810"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1121)
                     /locus_tag="PK224_21550"
     CDS             complement(288..1121)
                     /locus_tag="PK224_21550"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRB18391.1"
                     /translation="MADSTTQSVCWGTVALFTAITLLTFVGVPLFAYFYDYTLLDWVL
                     LAVLYIVTGMGITVGYHRMITHRSFESHPWVKACLLVMGGWAVQNSALMWCADHIRHH
                     AHTDEEADPYNASKGFWHSHVGWLFFDTPYREDRFAAKLRTDPMIMWQHRYYWVIVAS
                     GFLLPFALGYLNGGFTSGLGCFLLAGVLRTVLVLNSTFTINSLCHMVGTQPHGTQDSS
                     RDSWLISFVSFGEGYHNYHHTYSHDYRNGSKWYNFDPSKWLIYSLSLFGLTYNLHRER
                     T"
     gene            1508..>2056
                     /locus_tag="PK224_21555"
     CDS             1508..>2056
                     /locus_tag="PK224_21555"
                     /inference="COORDINATES: protein motif:HMM:NF037039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18392.1"
                     /translation="MPLDPAPLEPRIRAIGEDLARRSGGRAPGLFDSRWWSQAAINLA
                     MKDPAFKAQLFRFIDVLPSLHDDARVVQLAEEYFGEMSAHLFGAQWGLKALASTKLGA
                     SLSGKAIRHQVEQMATSFIAGASIEQAVPTLRKLWEDGRAFSVDLLGEASVSEREADA
                     YRDRCLAALQALGEACPGWSPAP"
BASE COUNT          428 a          567 c          650 g          411 t
ORIGIN      
        1 gcggtgcgcc accttgttgc tctgccatag atctgcctcc taccacctcg cgggtcgggg
       61 tggaattcgc gggtttcatt gcatattaag ctatcgtccc ggggaaaaga aatgggccag
      121 tagacgcagt gcgcagctgg gtagaagagg gcgagatgaa agggagggac gaaacgtctc
      181 gggacgttca gtgatcagtg ctcacggttg aatggagcag gtaagaacga gaatcgagca
      241 tacctgaacg acgcgaggga tgtttcacct ttcacgtctc acgcctgcta ggtgcgttcc
      301 ctgtgcaggt tatacgtcag gccgaacaac gacaacgaat agatcaacca tttcgacggg
      361 tcgaagttgt accacttcga tccgtttcga tagtcgtgcg aataggtgtg gtgatagttg
      421 tgatagccct cgccgaagct gacgaatgag atcaaccagc tgtcgcggct gctgtcctgc
      481 gtgccgtggg gttgggtgcc gaccatatgg cagagggagt tgatcgtaaa cgtggagttc
      541 aggaccaaca cggtccgcag gacgccggcg aggaggaaac agcccagccc gctggtgaag
      601 ccaccgttca ggtatcccag cgcaaatggt agcaggaagc cggaggccac gatcacccag
      661 taataccggt gttgccacat gatcatcggg tcggtacgta gtttcgctgc aaaccggtcc
      721 tcacgatacg gggtatcgaa aaacaaccag ccgacgtgac tgtgccagaa tcccttcgac
      781 gcattgtagg gatcggcctc ttcgtccgtg tgcgcatggt ggcggatgtg atcggcgcac
      841 cacatgaggg cggagttctg cacggcccag cctcccatca ccaggaggca ggctttcacc
      901 cagggatgac tctcgaagct gcgatgggtg atcatgcggt gatagccgac cgtgatcccc
      961 atgccggtca cgatatagag gacggcgagc aggacccaat cgagcagggt gtagtcatag
     1021 aagtaagcga aaagcgggac gccgacgaag gtcagcagcg tgattgcagt gaacagggcc
     1081 acggtgcccc agcagacgga ttgtgtggtc gagtcggcca tcgtactcct tcggaacctg
     1141 tgcgcaactc atcgccacag tgcaccgcca tgcaaatggt gtaggtcaag gcaaacggcg
     1201 catgctaccg gagcggcagg cggattttca agcacagaag tgtcgcgaga tgacacaacg
     1261 gccgattagc tgggacgtcc tgaagaaatc gttcgctggg gcgatacgca tatgccggtt
     1321 ccgctagtta cgtacactcg actccgcgtg cctgtggttg agccaaatcc gtatcccgct
     1381 gttcctggca acgatgcgaa atccatcctg cgtaagatgg accgcggcct attcgtcctc
     1441 aaatattcaa gtcgcgcgct gcggtgtata ctgcagagag cctctcattt tggttcagga
     1501 ggaaccgatg cctctcgacc ctgccccgct cgaaccccgt atccgcgcga ttggtgaaga
     1561 cttggcccgg cgttccggcg ggcgggcgcc cggcctgttc gattcgcgct ggtggtcgca
     1621 ggcggccatc aacctggcga tgaaagatcc cgcgttcaag gcgcaactgt tccggtttat
     1681 cgatgttctg ccctcgttgc acgacgatgc acgggtcgtg cagttggccg aggaatattt
     1741 cggcgagatg agcgcacacc tcttcggtgc ccaatgggga ctcaaggcgc ttgcctccac
     1801 caagctgggc gcatcgctca gtggaaaggc catccggcat caggtggagc agatggccac
     1861 cagtttcatc gccggtgcgt ccatcgagca ggccgttccg acgctccgta agttatggga
     1921 ggacgggcgg gccttttcgg tggatctact gggtgaagcc agtgtcagcg agcgagaggc
     1981 ggatgcctat cgtgatcgtt gcctcgcggc tttgcaggcc ctcggtgagg cctgtcccgg
     2041 ttggtcccct gcgccg
//
LOCUS       DAORRQ010000196         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1114_length_2056_cov_4.694653, whole genome shotgun sequence.
ACCESSION   DAORRQ010000196 DAORRQ010000000
VERSION     DAORRQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1114_length_2056_cov_4.694653"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PK224_21560"
     CDS             <1..792
                     /locus_tag="PK224_21560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRB18393.1"
                     /translation="DLRAQQAQAVLAKTVLDRRRALTQDQAAAQEEVDRARADLDVAH
                     AQMARTKALIARKTIRAPFRARVGLADVHPGQYLNEGTQLTTLQGIDEAVHVDFAVPQ
                     HVALGLREGANVEVFTGDSSPVAARIVAIDARIDPATRNTMVRARVEHSATMPAPGAA
                     VRIRVPVGLARKAVAVPVNALKKGPGGDQVFVIEPDRENKQRAHVRQVESGTMLGDDI
                     VIYSGLNAGERVAASGAFKLRDGVLVVAAGEPARTPEPDHLLSQQ"
     gene            810..>2056
                     /locus_tag="PK224_21565"
     CDS             810..>2056
                     /locus_tag="PK224_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB18394.1"
                     /translation="MRSFTDIFIKHPVLAVVVNLVIVLVGWRALTTLPVQQYPKIESS
                     SIIITTVYYGAAAETVRGFLTTPIERVVSAISGVDYLESTSRAGVSTVTVHLKLNHNS
                     TAALAEVTARLQQVRSELPAEAEPPAVEVQRADRPYASFYLSFTSPERTVPALTDWLL
                     RTLQPQLATLTGVQRVTIEGGRQIAMRVWIDPDRLAAYNLSPGDVQAALRRNNYLAAV
                     GRTKGNLVQVNLLANTDLRSVNEFQELIVADRGGAIVRLRDVARVENGAEEAEMVAKY
                     NRLEGVYLGVWPLVGSNEIEVAHRLREEMDRIRPTLPKDIDMQLVWDGTMFMESALTE
                     ITKTLGETILIVATVVFLFMGSIRTALVPLVAMPVSLIGAAIFMYAFGFSLNLLTILA
                     IVLSVGLVVDDAIVVVENVERHVR"
BASE COUNT          370 a          601 c          702 g          383 t
ORIGIN      
        1 gacttgcggg cccaacaggc tcaggcggtc ctggcgaaga ccgtgctgga tcgtcggcgg
       61 gcgttgactc aagaccaagc cgcggctcag gaggaagtcg atcgggcccg cgcagacctc
      121 gatgtcgcgc atgcccagat ggcgcgcacg aaggccttga tcgcccgcaa gacgatccgt
      181 gccccgttcc gcgcccgtgt gggattggcc gacgtgcatc ccgggcaata tctcaacgaa
      241 ggcacgcaac tgacgacgct gcagggcatc gatgaggcag tccatgtgga ctttgcggtt
      301 ccgcagcacg tcgcgctcgg attgcgggaa ggggcgaacg tcgaggtctt caccggtgac
      361 tcctccccag tggccgccag gattgtggcg atcgatgccc gcatcgaccc cgccacgcgc
      421 aacaccatgg ttcgtgccag ggtcgagcat tccgccacca tgccggctcc cggcgcggcc
      481 gtgcggatca gggtgccggt ggggttggcg cgcaaggctg tggcggttcc ggtgaatgcg
      541 ttgaaaaagg gtccgggtgg cgatcaggtg tttgtcatcg agccggaccg cgagaataaa
      601 cagcgcgcgc atgtgcgtca ggtcgaaagc gggacgatgc tcggtgacga catcgtcatc
      661 tatagcggtt tgaacgccgg cgaacgggtg gccgcgtcgg gagcgttcaa gttgcgcgac
      721 ggcgtgctcg tcgtcgccgc cggcgagccg gcgcgtacac cggaaccgga tcaccttctc
      781 agccaacagt aagtcagggg agcaggccca tgcgttcatt taccgacatc ttcatcaagc
      841 accccgtact ggccgtggtc gtgaacctgg tgatcgtcct cgtgggttgg cgcgcgctga
      901 cgacgctgcc ggtgcaacag tatccgaaga tcgagagttc ctcaatcatc atcacgacgg
      961 tctattacgg agccgcggca gagacggtgc ggggattcct caccaccccg atcgagcggg
     1021 tcgtgtcggc gatcagcggg gtggattatc tcgaatccac aagtcgcgcc ggcgtcagca
     1081 ccgtgacggt gcatttgaag ctgaaccaca acagcacggc cgcactggcc gaggtgacgg
     1141 cgcggctgca acaggtcagg tcggagctgc cggctgaagc ggaacctccg gcggtggaag
     1201 tacagcgtgc cgatcgcccc tatgcgtcgt tctatctcag tttcacgtct ccggagcgga
     1261 ccgtgccggc gctgacggat tggctgctgc gaaccttgca gcctcagctt gccacgttga
     1321 ccggggtgca gcgggtcacc attgaaggtg gacggcagat cgccatgcgc gtgtggatcg
     1381 atcccgatcg tctcgcggcc tacaacctct ctcccggcga tgtgcaggcc gcgttgcggc
     1441 gcaacaatta cctggccgcc gtcggccgga ccaagggcaa cctcgtgcag gtcaatctgc
     1501 tcgccaatac ggacctgcgt tccgtgaacg agtttcagga actcatcgtg gcggaccgtg
     1561 gcggcgccat cgtgcggctc cgggatgtgg cgcgagtgga aaacggcgct gaagaagcgg
     1621 agatggtggc gaagtacaac cgcctggagg gggtgtacct cggggtctgg ccgctggtcg
     1681 gctccaacga gattgaagtg gcacaccggt tgcgggaaga aatggatcgt atccggccga
     1741 ccctgccgaa agatatcgac atgcagttgg tctgggacgg caccatgttc atggaaagcg
     1801 ccttgacgga gattaccaag acgctgggcg aaacgattct gatcgtggcc acggtggttt
     1861 tcctgttcat gggatcgatc aggaccgcac tggtcccgct ggtcgccatg ccggtgtcgc
     1921 tgatcggggc cgccatcttc atgtatgcgt ttggcttcag cctgaatctg ttgacgattc
     1981 tggcgatcgt gttgtcggtg ggattggtgg tggacgatgc gatcgttgtc gtggagaacg
     2041 tcgaacggca tgtgcg
//
LOCUS       DAORRQ010000197         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1150_length_1991_cov_4.820764, whole genome shotgun sequence.
ACCESSION   DAORRQ010000197 DAORRQ010000000
VERSION     DAORRQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1150_length_1991_cov_4.820764"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1754
                     /locus_tag="PK224_21570"
     CDS             <1..>1754
                     /locus_tag="PK224_21570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRB18395.1"
                     /translation="IREYAQSLQQIRLAEFNEWMGHLAQWPAIVNLPSLAKFYGGRVI
                     EAMAAGRPVISWDIPGHPKNRGLFEPEKDILLFPQDAPEALAGQIDRILHDRALAESL
                     AQRAQGKIKRYHTAERRLQGTLEWIRTGQTPDYGLSEQANSPSAAQTTVPAPAAQSSP
                     SATGLPTADPISHPSAKVSMDQNAFYVDLFVNKPAWSTPEPNADEAARWSKIASFLEH
                     VVRQTRRETPGHTLRILDVGCGRGWLTNLATVYGTCEGIEPVAGVVEHARRMFPHIRF
                     EAGTPETVLARPDFMPFDIVLCSEVIEHVPHPEKPGFVSQLAQLLTSEGYLILTTPRG
                     DVWEEWRRIAPPNQPVEDWVTEQQLGQLLADGGFHHLGLERIPIEVPSLRYFPAATAH
                     DYRTKTLMPIYQVWACRRTAATGTHPVPFTRRPMVSVIVPTHNRPDRLRMALESLNSQ
                     QYQDFEVIVVNDGTTPVESVVAEMNCAGRTTLVNHDRNRGLAGARNTGLRHATGTYVA
                     YLDDDDRFLSDHLGTLVAFLERGTHQVAYTDAWRVTEQEAGAELRRDRPYSYEFDAQR
                     LLVHNYIPVLCLMHRRSCL"
     assembly_gap    1755..1764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1765..>1991
                     /locus_tag="PK224_21575"
     CDS             <1765..>1991
                     /locus_tag="PK224_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18396.1"
                     /translation="DESLFVHEDWDLWIRLATVYPFAHIAQTTAEFTWRTDGSSMTSH
                     DHDAFCRTTDIIYRKYFPYVAAQPTMLEAQR"
BASE COUNT          400 a          614 c          573 g          394 t
ORIGIN      
        1 atccgggaat atgcccaatc cttgcaacag atccgcctcg ccgaattcaa cgagtggatg
       61 gggcacctgg ctcaatggcc ggctatcgtg aacctgccga gcctcgccaa attttatggc
      121 ggacgggtca tcgaagccat ggcggccggt cgaccggtga tctcctggga cattcccggg
      181 catccgaaga acaggggact cttcgaaccg gagaaagaca tcctgctgtt cccccaggat
      241 gcgcctgaag cccttgccgg gcagatcgac cggattctgc atgatcgcgc attggccgag
      301 tccctggctc agcgggcgca gggcaagatc aaacggtatc acacagccga acgacgcctt
      361 cagggaacct tggagtggat tcgcaccgga cagactccgg actatggctt gtcggaacag
      421 gcaaactccc catccgcagc ccagaccacg gttccggcgc ctgcagctca atcctcgccg
      481 tccgccacag ggttgccgac ggcagacccg atttcccacc catcagccaa ggtgtctatg
      541 gatcagaatg ccttttatgt agacctattc gtgaacaaac cggcttggtc gacacccgaa
      601 ccgaacgccg atgaagcggc gcgctggtcg aagattgcgt cgttccttga acatgtcgtg
      661 cgacagacgc ggcgcgagac tcccggtcac acgctacgga ttctcgatgt gggttgtggc
      721 cggggatggc tgacgaatct ggcgacagtc tatggaacct gtgaaggcat cgagccggtg
      781 gccggcgtgg tcgagcatgc gcgtcggatg tttccccaca ttcgcttcga agcgggcaca
      841 cccgagacgg tgttggccag gccggacttc atgccgttcg atatcgtgct ctgttccgaa
      901 gtcattgaac atgtccccca tccagagaag ccggggttcg tcagtcaact ggcgcagctc
      961 ctcaccagtg aaggctatct cattctgacg actccgcgcg gggatgtgtg ggaagaatgg
     1021 cgacgcatcg ctccgcccaa tcagcccgtt gaggattggg tgacggagca gcagctgggc
     1081 caactattgg ccgacggcgg ctttcatcat ttggggctgg aacgcattcc gatcgaagtg
     1141 ccctccctcc ggtatttccc ggcggccacc gcccatgact atcgaaccaa gacactcatg
     1201 cccatttatc aggtctgggc ctgccgtcgc acggcggcga cggggacgca cccggtgccg
     1261 tttacacgac gaccgatggt gtcggtgatt gtgccgaccc ataatcgacc ggaccgtttg
     1321 cgcatggcct tggagagtct caactctcag cagtaccagg atttcgaagt gatcgtggtc
     1381 aatgacggca cgactccggt cgaatccgtt gtcgctgaga tgaactgtgc cggacgaacg
     1441 acgcttgtga atcatgaccg caaccgtgga ctggcagggg cgcgcaacac cggccttcgc
     1501 catgccaccg ggacctacgt cgcctatctc gatgacgatg accgcttcct gtccgatcac
     1561 ctgggcacgc tcgtcgcctt tctcgaacgt ggcacccatc aggtggccta caccgatgcc
     1621 tggcgtgtca ccgaacaaga agcgggagcc gaattgcgtc gtgaccggcc ctactcctat
     1681 gagttcgatg ctcagcgtct gcttgttcac aactatattc cggtgctctg tctgatgcac
     1741 cggcggtcct gcctnnnnnn nnnncgacga aagtctgttc gtccatgaag actgggatct
     1801 ctggatcagg ctcgccaccg tctatccgtt tgcgcacatc gcgcagacga ccgcagagtt
     1861 cacgtggaga accgacggct catcgatgac cagtcacgat cacgatgcct tctgccgcac
     1921 caccgatatc atttatcgaa aatactttcc gtacgtggcg gcgcagccga caatgctgga
     1981 ggcacaacga c
//
LOCUS       DAORRQ010000198         1980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1158_length_1980_cov_9.824416, whole genome shotgun sequence.
ACCESSION   DAORRQ010000198 DAORRQ010000000
VERSION     DAORRQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1158_length_1980_cov_9.824416"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..796)
                     /locus_tag="PK224_21580"
     CDS             complement(236..796)
                     /locus_tag="PK224_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB18397.1"
                     /translation="MPKHNTTNDGVVALIVPGIGNSGPGHWQTLWEERQPGWQRVQQR
                     DWDRPVCAEWLRGLDAAMARLSAPPVLIAHSMGCLLVAHWAKRSSPPVRAAFLVAVPD
                     PAGPMFPPAAQGFQPVPSEKLRFPSLVVASSNDPFGSVAYARRCAADWGSDFVEVGAV
                     GHINADSGLGDWPAGLALFDGFLKSL"
     gene            complement(783..>1980)
                     /locus_tag="PK224_21585"
     CDS             complement(783..>1980)
                     /locus_tag="PK224_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HRB18398.1"
                     /translation="AAEAALAGGAHVALYDSMASVGRKFLLAGKGGLNLTHSDPPDRF
                     LSRYGERRAQVAPWLAEFGADAIRTWARGLGVETYVGSSGRVFPTDMKAAPLLRAWLR
                     RLRQAGLMIHVRHRWHGWDEQKALCFETPQGTTSVRADAVILALGGGSWPKLGSDGAW
                     VPLLAGRSISIAPLRPANCGFDVGWTEHFRSKFAGHPVKTVGVVAKSPTGAVMRRMGE
                     FVITETGVEGGVIYAVASCLRDEIEATGRGTLRLDLAPDRELPRLIKDLSQPRGKKTM
                     ATHLQRRAHIDGVKAGLLREIVLKEDFADPTRLAAAIKALPLTLTAPRPLEEAISTAG
                     GVTCEALDKRLMLKALPGVFCAGEMVDWEAPTGGYLLTACFASGRTAGAGAVAWLKER
                     AGRAKA"
BASE COUNT          422 a          698 c          555 g          305 t
ORIGIN      
        1 ggccgctgag gcggcattgg ccggcggggc gcacgtcgcg ctgtacgatt cgatgtcctc
       61 ggaacggtct gccattttcc tcatcgactg ggatctgaaa gcaggaacac tcgcctcgcc
      121 ggaatatcga cagatcctgc tctagcaaca tgcgcggtga aacgcatcga gcgcatgccg
      181 tatcccgtcg ctcgtatctc gtcctgacga gatacgaacg cggacggcga gcgggtcaca
      241 gcgacttcaa gaagccatcg aataacgcaa gcccggccgg ccagtcaccg agaccgctgt
      301 ccgcgttaat gtggccgact gcaccaacct ccacaaaatc actgccccaa tcagcagcac
      361 aacgccgcgc ataggcgacg gaaccgaacg gatcgttgct gctcgccacc accagactgg
      421 gaaaccgaag tttctccgaa gggaccggct gaaaaccttg tgcagcaggc ggaaacatgg
      481 ggccggcagg atccgggact gcaaccagaa acgcagcacg aacaggaggc gaggaacgtt
      541 tcgcccagtg cgcgaccaac agacaaccca tgctatgcgc gatcagcaca ggcggagcag
      601 aaagacgagc catggcggca tcaagcccgc gcagccattc cgcacagacc ggacgatccc
      661 agtcacgctg ctgcactcgc tgccagccag gttgccgctc ctcccacaat gtttgccaat
      721 ggccgggtcc cgaattgccg atgccaggca cgatcagggc cacaaccccg tcgttggtcg
      781 tgttatgctt tggcacgccc tgcacgctcc ttgagccagg cgaccgcacc agcaccggct
      841 gtgcgcccgc tggcgaaaca ggccgtgagc agatacccgc ccgtcggcgc ttcccaatcc
      901 accatttctc ctgcgcagaa cactcccggc aacgctttga gcatcaaccg cttgtcgagc
      961 gcctcacagg tcacaccgcc tgccgtgctg atcgcctcct ccaaggggcg aggcgccgtc
     1021 agtgtaagcg gcaaggcttt gatcgcggcc gccagacggg tcgggtctgc aaaatcttcc
     1081 ttgagaacaa tctcacgaag cagcccggcc ttcaccccgt cgatatgggc gcgccgctgc
     1141 aaatgcgtgg ccatcgtctt ttttccgcgc ggctgagaga gatccttgat caaacgcggc
     1201 agttcacggt ccggcgccag atccaaccgc agcgtgccac ggccggtggc ttcaatttca
     1261 tcgcggaggc aagaggccac ggcatagatc acaccgcctt caacaccggt ctcagtgatc
     1321 acgaactccc ccatgcggcg catcacggcg ccggtcggtg atttcgccac cacgcccacc
     1381 gttttcaccg gatgccccgc gaacttgctc cggaagtgtt cggtccaccc cacatcaaac
     1441 ccgcaattcg ccggtcgcaa cggagcgatc gaaatggaac gccccgcgag caatggcacc
     1501 cacgcaccat cagagccgag cttgggccag ctgcctccac ccaacgccag gatgaccgca
     1561 tccgcccgta cggaggtcgt accttgcggc gtttcgaaac agagggcctt ctgttcatcc
     1621 cagccatgcc aacgatgccg cacatgaatc atcaatcccg cctgacgcaa ccgccgtaac
     1681 caggcgcgca gcagcggtgc agccttcata tccgtcggaa acacacgacc ggaggagccg
     1741 acataagtct cgacaccaag cccgcgcgcc cacgtgcgaa tcgcgtcggc cccgaactcg
     1801 gccaaccagg gcgcgacctg cgcgcggcgc tcgccatacc ggctgagaaa acgatcgggc
     1861 ggatcggagt gagtcaggtt cagcccgcct tttccggcca acagaaactt gcgccccacg
     1921 gacgccatcg aatcgtacag cgcgacgtgc gccccgccgg ccaatgccgc ctcagcggcc
//
LOCUS       DAORRQ010000199         1953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1175_length_1953_cov_473.195469, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000199 DAORRQ010000000
VERSION     DAORRQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1175_length_1953_cov_473.195469"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1873)
                     /locus_tag="PK224_21590"
     CDS             complement(74..1873)
                     /locus_tag="PK224_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRB18399.1"
                     /translation="MSRATVSMLPVSECSQAVSPLAQTRSAACYHRLVEQGFLQQEEL
                     VLATAEAARKRLDVASVLMERYRIPKPALGAALAEFYHCPFLAYDERTVMERELLKNL
                     SLDYLRMNHWVPLRRQGNGIEVLIDDPHNPDKLFDVRRAFPGQALSYRVGLRCDIARL
                     LNAIQGRESGDAISDILGELVSEAQLEEQQNATIAAITENDSAIVRLANQIITDAYRR
                     GASDIHIEPYADRKETAVRFRVDGTCFTYMKIPAAYRRAIVSRLKIMASLDIAERRKP
                     QDGKIRFKLGTGQEIELRVATLPTSGFNEDVVIRLLTAHGARRLEDLEFSDGTRRLVG
                     QLAQKPHGIVLCAGPTGSGKTTTLHAILASINTDERKIWTAEDPIEITQDGLRQVQVH
                     PKIGLTFATTMRAFLRADPDVIMIGEMRDKETADIAIEASLTGHLVFSTIHTNSAVET
                     VVRLLDLGCDPFNFSDAMLGVLAMRLCKRICPNCREAYHPTSSEYDELVQAFGEGDWE
                     GVHPAYNPGLTLFRGRGCDTCNQTGYRGRVPIHELMVVSDSMKALIQGRARTGELLTL
                     AKSDGMRTLVQDGIEKVLQGLTTYKQVRAVAIK"
BASE COUNT          415 a          600 c          507 g          431 t
ORIGIN      
        1 atccgaacgg cgctttcgaa tgttcatcgc aacctcctcc gtcaagatgt tgcaacgacc
       61 acttgaatcc gccctatttg atcgcaactg cccgcacctg tttatacgtg gtgagtcctt
      121 gcagcacttt ctcgataccg tcttgcacca acgtcctcat cccgtcgctc tttgcgagtg
      181 tgagtagttc cccggtgcgc gccctgccct gaatcagtgc cttcatggaa tcagacacca
      241 ccatcaactc atggatgggc actcgtccac ggtacccggt ctggttacac gtgtcacacc
      301 ctcgtccacg aaacagtgtg agccccggat tatatgcagg atgcacgcct tcccaatcac
      361 cctccccaaa ggcctgcacg agttcgtcat attcgctgct cgtgggatgg taggcctcgc
      421 ggcagttggg acagatgcgc ttgcacaacc gcatcgccaa cactcccaac atggcatctg
      481 aaaaattgaa cgggtcgcac cccagatcga gcaatcgcac cacagtctcg acggcgctat
      541 tcgtatgaat ggtactgaac accaagtgcc cggtcagaga ggcttcaatg gcaatatccg
      601 ccgtctcctt gtcccgcatt tccccgatca tgatcacgtc cggatctgcc ctcaggaagg
      661 ccctcatggt cgtggcaaaa gtgagtccaa tcttgggatg aacctgcacc tgccgcaatc
      721 catcttgcgt aatctcgatc ggatcctccg ccgtccagat ctttcgctca tcggtattaa
      781 tcgacgccag aatggcatgt aaggtggtgg tctttcctga tccggtcgga ccggcgcaca
      841 ggacgatgcc atgcggcttt tgagccaact gtcctacgag ccggcgtgtg ccgtcgctga
      901 actccagatc ctccaagcgg cgagctccgt gagcggtcag caggcgaatc acgacatctt
      961 cattgaagcc cgaagtggga agtgttgcca ccctcaattc gatctcctgc cccgtaccca
     1021 acttgaaccg gattttgcca tcctgcggct tgcgccgctc ggcaatgtcc agactggcca
     1081 taatcttcag gcgggagacg atcgcccggc gataggccgc cggaatcttc atgtacgtaa
     1141 agcaggtgcc gtcgacgcgg aagcgcacag ccgtttcctt tcgatcggca tagggctcaa
     1201 tatggatatc cgatgccccg cgccgatagg cgtcggtaat gatctgattg gcgagccgga
     1261 cgatggccga atcgttttcc gtgattgccg caatcgtcgc attttgttgt tcttcgagct
     1321 gcgcttcact gaccagctcg ccaagaatgt cggatatcgc atcgcccgac tcgcgccctt
     1381 gaatggcatt caacaaccgc gcaatatcgc aacggagtcc gacacgatag gacagtgcct
     1441 ggcctggaaa cgctcgccgc acatcgaaca gcttgtccgg gttgtgcggg tcgtcgatca
     1501 ggacttcgat tccgttgccc tgacgcctga gcggaaccca atggttcatc cgaagataat
     1561 ccaagctgag attctttaat aactcccgct ccatgaccgt gcgctcgtca taggcgagaa
     1621 acgggcagtg atagaattct gccagtgcgg caccgagtgc cggcttcggt atccgatacc
     1681 gctccatgag cacagaagcc acatcgagcc gctttcgcgc ggcttcagcc gtggcaagca
     1741 ctaactcctc ctgttgtaga aaaccctgtt cgacaagccg gtggtagcaa gcagcggagc
     1801 gcgtctgcgc caaagggctg accgcttggg aacactcact gactggtaac atggacaccg
     1861 tggcacgact catgggaccg attcctttct gaataccatg gagacgattc gattgtagat
     1921 gattgtctga actcaccatg ctggcagagt gac
//
LOCUS       DAORRQ010000200         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1176_length_1950_cov_8.510290, whole genome shotgun sequence.
ACCESSION   DAORRQ010000200 DAORRQ010000000
VERSION     DAORRQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1176_length_1950_cov_8.510290"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..>1950)
                     /locus_tag="PK224_21595"
     CDS             complement(255..>1950)
                     /locus_tag="PK224_21595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRB18400.1"
                     /translation="VNDERLVVGVGADDGWSNLRWTETRLFAINRDGSNLKTDLITPS
                     RDHNSFTREYLPQLQDRVIGKIPGDNRSVLIALDLRIGTYPDVYKLDVYTGRRELIEG
                     STYGMRDWMADRQGVVRLGTAVEGTMVHILVKPVGQDRWQTLRKYDALREPDMTPLGF
                     DEDPNMLFVAQRLDGRTAVYSIDISNPQVPPTLRASHALYDIEGGLIYSSWLKQVVGV
                     RSRLDESASIYWNPKAKALQTRVDLALPGRINDIQGSSRNGRRHIVVSSHATQPPQWY
                     LYDEDLNRLILMVDGYPKLVPNELVKPMPLTLKARDGTVLHGYVTHPAHAQQPGPLIV
                     LPHGGPAGRDVLDFNYWTQFLASRGWAVLQVNFRGSSGYGGEFLQAGFKRWGLEMQDD
                     LTDAAHYAIEQGIADPERICIVGGSYGGYAALMGIVKTPELFRCAVSFAGVSDLRALL
                     TDKRRFLGYELGAERQLGAWWSDRDRLKATSPVNHAEKIRTPLLIVHGAEDRTVPVEQ
                     SRAMVDALKEAGFKHMRYIELPDGDHHLSRQEDRLTFFREMERFLATHLDQAGGSE"
BASE COUNT          419 a          603 c          519 g          409 t
ORIGIN      
        1 aaagatggaa tcattcacaa aacgaagcag gaagccgacg cggatcacga tcatcatttt
       61 gacggtcgga cttcaatgaa atagggctgt ttacgggtca tatcctcggc gcgcgggcga
      121 gtaactccat gagctgacga gagactcgtc gggccgctgc acgaaggtct tgtcgaaggc
      181 cagccctgcc tcgctctgca aacctgccgg acagtttgaa tcggacctct ccccatcagc
      241 atacatggtc aagttcattc agaccctccc gcctgatcga gatgggtggc gagaaacctc
      301 tccatttcac ggaaaaatgt gagacggtct tcttggcggc tgaggtgatg atcgccatcc
      361 ggcagttcga tataacgcat gtgcttgaaa cccgcttctt tcagagcatc gaccatcgcg
      421 cgagattgtt cgacaggcac agtccgatcc tccgccccgt gaacgatcaa gagcggggtg
      481 cgtatcttct cagcatggtt gaccggggac gtggccttca gccgatcgcg atcggaccac
      541 caggcgccta actgccgctc cgctcccagc tcatacccca aaaaccgccg cttgtccgtc
      601 agcagcgcgc ggaggtccga cactccagca aagctcaccg cgcaacgaaa gagctccggt
      661 gtcttgacga ttcccatcaa tgccgcatag cccccataac tcccacccac gatgcagatg
      721 cgttcagggt ccgcgatgcc ctgctcgatc gcgtagtgtg cggcatcggt taagtcgtcc
      781 tgcatttcga gtccccaccg cttgaatccc gcctgcaaaa actccccgcc gtagccgctg
      841 gatcccctga agttcacttg cagcacggcc cagccgcgac tggcgagaaa ttgggtccag
      901 taattgaaat ccagcacatc tctgccggcc ggcccgccgt gaggcaacac aatcaacggc
      961 ccaggctgct gagcatgcgc cggatgcgtc acgtacccat gcaacaccgt gccgtctctc
     1021 gccttgagag tgagcggcat gggcttgacc aattcattcg ggaccagctt cggatacccg
     1081 tccaccatca aaatcagccg gttgagatcc tcgtcgtaca ggtaccattg aggcggttgc
     1141 gtcgcatggc tggagacgac gatatgtctg cggccgttcc gactgcttcc ttgtatgtca
     1201 ttgattcgtc caggaagggc cagatcgacc ctggtctgca gtgctttcgc cttaggattc
     1261 cagtagatgc tggcagattc gtccaggcga ctgcgaacac cgacaacttg tttgagccag
     1321 gacgaataga tgagaccgcc ctcgatatcg tacaaggcat gggatgcgcg gagcgtaggc
     1381 ggcacctgcg gattagaaat gtcgatgctg tagactgcag ttcttccatc gagacgttgt
     1441 gccacaaaga gcatgttggg atcctcgtca aatcccagag gagtcatatc cggctcacgt
     1501 agtgcatcat acttccgcaa cgtctgccag cgatcctggc ccaccggttt gaccaggatg
     1561 tggaccatag tcccttcaac ggctgttccc agccgcacga caccctgtcg gtcggccatc
     1621 caatcccgca tcccataggt gcttccctcg atcaactccc ggcggccggt gtacacatcc
     1681 aatttgtaga catcgggata cgtcccgatc ctgagatcga gtgcgatcag gacggatcgg
     1741 ttgtccccgg gaatttttcc gatgacccta tcctgaagct gcggaagata ttctctggta
     1801 aaagaattat ggtcgcgact cggagtgatc agatccgtct tgagattcga gccgtcgcga
     1861 ttgatggcga agaggcgcgt ctccgtccac cggagattgc tccacccatc gtctgccccc
     1921 acgcccacca ccaaccgttc gtcattgacc
//
LOCUS       DAORRQ010000201         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1195_length_1925_cov_1264.459358, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000201 DAORRQ010000000
VERSION     DAORRQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1195_length_1925_cov_1264.459358"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK224_21600"
     CDS             complement(<1..539)
                     /locus_tag="PK224_21600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRB18401.1"
                     /translation="MIRGIKNYLRVLGYGGFLSAVESKVTQSTVYFKLNREDCKYPFK
                     LRIPSSDVSTYQQVFLNKEYDFLADTQPKVIIDAGANIGLASIYFANKYPQATIIAIE
                     PELNNFELLKENVASYSNISAVQAALWNKNEEIDLVDPGLGNWGFMTESKQSSESLLR
                     NACHTVSAMTLDRIIDEYNL"
     gene            complement(660..1403)
                     /locus_tag="PK224_21605"
     CDS             complement(660..1403)
                     /locus_tag="PK224_21605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB18402.1"
                     /translation="MAETVQTSGATSLGGLHDVMTQIIRRWAKKAVLEILPDSGWLRS
                     LTYRPMFETWCRCYLGTHPVFSERTEMYNFINAVIISNKPICYLEFGVYKGESIKHFA
                     QLNSDQDSEFIGFDTFTGLPEDWVEFSRKVGAKTFDAGGECPKFDDRRVSFMKGLFQD
                     TLPIFLKEYKSNFQLVIHNDSDLYSATLYVLTRANDIITPGTVIIFDEFYSVMHEHRA
                     LQDYCSAYIRNYEVIAATEGYKQIAIRMR"
     gene            complement(1432..1677)
                     /locus_tag="PK224_21610"
                     /pseudo
     CDS             complement(1432..1677)
                     /locus_tag="PK224_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          477 a          472 c          439 g          537 t
ORIGIN      
        1 agattgtatt catcgattat cctatcaagg gtcatggccg aaacggtgtg acaagcgtta
       61 cgaagtagac tttctgacga ttgcttactc tctgtcatga agccccagtt ccccagcccc
      121 ggatcaacta gatcgatttc ttcgttcttg ttccacaacg cagcttgaac ggctgaaata
      181 ttagagtacg aagcaacgtt ttccttaagt agctcaaaat tgtttagttc tggttctatt
      241 gcgataatcg tagcttgggg atatttattt gcaaaatata tcgaggcaag tcctatgttc
      301 gcacccgcat cgattatgac ttttggttgt gtatctgcta gaaaatcata ttctttatta
      361 aggaacactt gctggtatgt tgagacgtcc gatgaaggaa ttcgtaattt gaaaggatac
      421 ttgcaatcct cccgattcaa tttgaagtaa actgtggact gcgttacttt ggattctaca
      481 gcggatagga agccgccata acctagaacc ctgagatagt tctttatacc ccgaatcata
      541 ctttctccta cctgcaggta atatgacctt ccccgatttg atggacaggt tacgcgactg
      601 cggccctatt ttacccttgc caacgagacc gggtaaattt ccggcttctg aataaggact
      661 caccgcattc tgatagcaat ttgtttatag ccttcagtcg cagcgatcac ttcatagttc
      721 ctaatgtagg cagaacagta atcttgcaat gctctgtgct catgcatcac gctataaaac
      781 tcatcaaaaa tgataacagt accaggggtt atgatgtcat ttgctcgggt caaaacatag
      841 agtgttgcgg agtataggtc cgaatcatta tggataacca attggaagtt tgatttgtat
      901 tctttcagga agatcggcaa tgtgtcttga aatagtcctt tcatgaacga tactcgccta
      961 tcatcaaatt ttgggcactc gccaccagca tcaaatgttt tcgctcccac tttgcgggaa
     1021 aattctaccc aatcctctgg tagaccagta aatgtatcaa agcctatgaa ttctgagtcc
     1081 tggtcggaat taagctgagc gaagtgtttg atactttcac ccttatatac accaaactct
     1141 aagtagcaaa ttggtttatt cgaaatgatt accgcgttta tgaaattata catttcagtt
     1201 ctttcactaa atacaggatg ggtgcctaaa taacatctac accatgtttc aaacataggt
     1261 ctataggtga gggatctcaa ccaacctgag tcaggcaata tttccaatac agccttcttg
     1321 gcccatcttc ttattatttg cgtcattacg tcgtgtaatc cccccagaga ggtggctccg
     1381 ctagtttgga cagtctctgc catttcataa atggcgcctg gtggtgtgag cctacgcggc
     1441 cgtgggccgt gcaggcaggt gctcccagta cacgcgatcg ggcgtgcgtc cgtcaagcgc
     1501 gcgatgcggc ctgatctgat tatagaaggt catgtagcgg gccaccccgt cctgagcgtc
     1561 gcggaccgtc tcataggcat ggcgatagac ttcctcatat ttgagactcc gccacaaccg
     1621 ctcgacaaag acattgtccc gccaccgccc gactccatcc atgctgatct gaattccctg
     1681 agtctgtgac ccgccccgca aatgtagacc gatcgagacc tgaggatttg gcctgggcgt
     1741 gatcggtttg agccgcctgt gcggcgatac gaacttagca tagctcatgt gagcgcctcc
     1801 agtaattcga gtgcggcggg gactctctgg ggcagcgctg accagttcgc ggcaaaggcg
     1861 gctggtgtca tgcgtattcc gctgaatccg gccaccgatt ccgagcgaat ccggccaccc
     1921 ctcgg
//
LOCUS       DAORRQ010000202         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1214_length_1885_cov_8.114208, whole genome shotgun sequence.
ACCESSION   DAORRQ010000202 DAORRQ010000000
VERSION     DAORRQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_1885_cov_8.114208"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>652
                     /locus_tag="PK224_21615"
     CDS             <1..>652
                     /locus_tag="PK224_21615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB18403.1"
                     /translation="QQQNNLAPWVGQAGRVAWLDRLEDLPPNSVTGLFFSNELVDAFP
                     VHRLAVLDGRPQEVYVEYRDERFSEVYRPLSNELTAYLREGGINLPDGYRAEINLDAV
                     DWMTQVAQVMARGAVLTIDYGHTAEDLYGPDRKNGTFLCYYHQTTSEDAYDRVGEQDM
                     TAHVDFTALAQTGQQAGLDVTGFTNQMSFLIGLGAEQLLESLQPESPEFYAAIHLLR"
     assembly_gap    653..662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            808..1179
                     /gene="ndhC"
                     /locus_tag="PK224_21620"
     CDS             808..1179
                     /gene="ndhC"
                     /locus_tag="PK224_21620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRB18404.1"
                     /translation="MGSEAAPLNYIPILIFIVIAFAFGAVQILLGRIVRPSRPYRAKL
                     APYESGSPLFSDARVQFPIRYYIIAMLFVIFDIEIVFMFPWAVAFKKLGLVGLVEMVV
                     FIGILIVGFWYAWKKGALEWD"
     gene            1277..1756
                     /locus_tag="PK224_21625"
     CDS             1277..1756
                     /locus_tag="PK224_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRB18405.1"
                     /translation="MSFLERQLDANIMTTNLDALVCWARKSSLWPMTFGLACCAIEMI
                     ASVSSRYDIDRFGAGVFRASPRQSDLMIVAGTVCRKMAPVIRRIYDQMPEPRYVISMG
                     SCATSGNHYNSYAVVQGVDQIVPVDVYIAGCPPRPEALLDGLLKLQEKIQREKIFVK"
BASE COUNT          378 a          546 c          541 g          410 t
ORIGIN      
        1 ccagcagcaa aacaatctgg cgccctgggt gggacaggcc ggtcgcgttg cctggctcga
       61 ccgcctggaa gatctgccgc cgaacagcgt caccggactc tttttttcga atgaactcgt
      121 agatgccttt cctgttcacc ggctagcagt gctcgacggc cggccgcaag aggtctatgt
      181 cgaatatcgc gacgagcgat tcagcgaggt ctaccgcccc ctctccaatg aactcaccgc
      241 ctacctgcga gagggcggga tcaacctgcc ggacggctat cgggctgaaa tcaatctcga
      301 cgcggtcgat tggatgacgc aagtggcaca ggtgatggcg cgcggcgccg tgctgacgat
      361 cgattacggc cacaccgccg aagacctcta tggcccggac cgcaagaacg gcacgttcct
      421 ctgctactac caccaaacca cctctgagga tgcgtacgac cgggtggggg agcaggacat
      481 gaccgcccac gtggacttca ccgccctggc gcaaacgggc caacaggccg gattggacgt
      541 caccgggttt acgaatcaga tgagtttctt gatcggcttg ggcgccgaac aattactgga
      601 atcgctccag ccggaatcgc cggagtttta cgcggcgata cacctgctgc ggnnnnnnnn
      661 nnttcaaagt attgatacag cacaagggca tggccaagcc cgagttggac ggactgaagt
      721 tccagccgtt cttcggatcg attttgcgaa caaacgcgcc ggtacacgac gcttcacgaa
      781 cgacgcttca cgattcacag gtctgtcatg ggtagcgaag ccgcaccgct gaactacatc
      841 ccgatcctga tttttatcgt gatcgcgttt gcgttcggcg ccgtccagat tctcctggga
      901 cgcatcgttc gtccaagccg gccctatcgc gccaaactcg cgccgtacga gagcggcagc
      961 ccgctcttct ccgatgcccg ggtgcagttc ccgatccggt attacatcat tgcgatgctg
     1021 ttcgtgatct tcgacatcga aatcgtcttc atgtttccct gggccgtggc gttcaagaag
     1081 ttgggactgg tggggctcgt cgaaatggtc gtgtttatcg gcatcctgat cgtcgggttc
     1141 tggtacgcct ggaaaaaggg ggcgctcgaa tgggactaac ggccgtaaaa cgagagacgt
     1201 caaacgtgca cggtgcggac gatgctctgc cgtctttcct ttcacgtttc acctttcacg
     1261 ttccacaagg tcagatatga gttttttgga acggcagctt gatgccaata tcatgacgac
     1321 gaatctcgac gccctcgtct gctgggcgcg caaatcttct ttatggccca tgacgttcgg
     1381 cctggcctgc tgcgcgatcg agatgattgc gagcgtctcc tcgcgctacg acatcgaccg
     1441 attcggcgcg ggtgtcttcc gcgcatcgcc tcgccaatca gacttgatga ttgtggcggg
     1501 aacggtctgt cgaaagatgg cgccggtcat tcgccgcatc tatgatcaga tgccggaacc
     1561 gcgttacgtc atctccatgg gatcctgcgc gacctccggc aatcactaca acagttacgc
     1621 cgtcgtgcag ggcgtggacc agatcgttcc ggtcgatgtg tatattgccg gttgcccgcc
     1681 gcggcctgaa gcgctgttgg acgggttgtt gaaattgcag gaaaagattc agcgcgaaaa
     1741 gatctttgtg aagtgagaga ggaacagagg agtatcgctt gtgagcgcga gcttctctta
     1801 ccatcggcta taggccatcg gctctttgag gaagatgcaa ccgctgcttg aacgactcat
     1861 gactaccttt cccgacgccg tccgc
//
LOCUS       DAORRQ010000203         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1224_length_1876_cov_74.395387, whole genome shotgun sequence.
ACCESSION   DAORRQ010000203 DAORRQ010000000
VERSION     DAORRQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1224_length_1876_cov_74.395387"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..696)
                     /gene="coaE"
                     /locus_tag="PK224_21630"
     CDS             complement(94..696)
                     /gene="coaE"
                     /locus_tag="PK224_21630"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRB18406.1"
                     /translation="MILVGLTGGVATGKSTVAKMFERCGALVIDADALAHQVVEPGKP
                     AWRAIIKTFGTAVLNPDRTLNRQALSAIVFRNRSKLRRLEQIIHPRVAREQTRLTRQA
                     ARNDPKAIVIYDVPLLYEAGIDKRVDKVIVVTADRETQITRLNKRNGFTRAEALRRIR
                     SQMPIKQKAAAADYLLDGTTPRPRLLTLVKRLYRELHTLA"
     gene            781..1821
                     /locus_tag="PK224_21635"
     CDS             781..1821
                     /locus_tag="PK224_21635"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HRB18407.1"
                     /translation="MKNMKNCLFAVLFVCLSASAQVLSLPIAQAEEPASVERQPLVVA
                     LDGSGQFLSIQEAVDAAKKGDTVLIRPGAYAEDVTIHSKENVRLIGAGMDQVTILGRE
                     RVGVFHVGKWPYGATNIEISGITINEHGGHAMGMFNGRGIVLHHVRVKGMLFTQQVED
                     VRIEDCIIGGSETTGVQFANSQAVMRSNFIHDNDHGVSVAGKSTVRLERNVITRSLFE
                     AVIVNDQAKATLLGNTFVKNGGGAAFLGTSQNEASGNIVSLNAYGFVVAPSSRVLFSY
                     NAMQNSGSNYLRSGTPNQPAPELKPDSDLTVDPRFVDTARDDFRLRADTALVKIGEFP
                     YLGALPPLAGSH"
BASE COUNT          362 a          518 c          604 g          392 t
ORIGIN      
        1 tgtcgaccga ccgagcggcg agcccgcccg ccgacaggcc tgtcgcagcc gcgaatccgc
       61 gattgcagca gaagcgctca tgaataatgc gggttaggcc agggtgtgaa gctcgcggta
      121 gagccgtttg accagcgtca gcaggcgtgg gcggggcgtg gtgccgtcga ggaggtagtc
      181 cgccgctgcg gctttctgtt tgatcggcat ttgactgcga atgcgtcgga gggcttcggc
      241 gcgggtgaat ccgttgcgtt tgttcaggcg ggtgatctgg gtttcacggt cggcggtgac
      301 gacgatgacc ttgtccacgc gtttgtcgat gccggcttcg tagaggagcg gaacgtcgta
      361 aataacgatg gccttgggat cgttgcgcgc ggcttgcctg gtcagtctgg tctgttcacg
      421 tgcgacgcgg gggtggatga tctgctccag ccgacgcaac ttggaccggt tccggaagac
      481 gatggcgctc agggcctggc gattgagggt acgatcggga ttgagaacgg ccgtgccgaa
      541 ggtcttgatg atggcgcgcc aggccggctt gcccggttcc accacctggt gggccagcgc
      601 atcggcgtcg atgacgagcg ccccgcatcg ctcgaacatc ttcgctaccg tgctcttgcc
      661 ggtcgcgacg ccgccggtga ggcccacaag gatcatgggg cgggaagata ccatgggggg
      721 cgagggcccg caatgacgct cgattgactt ctcgccatgc ccggcggtat gaccatttgt
      781 atgaagaaca tgaaaaattg tctattcgcc gtcctctttg tgtgtctatc ggcctcggcc
      841 caggtcttga gccttcccat cgctcaggct gaagagccgg cgtccgttga acggcagccg
      901 ctggtggtgg cgctggacgg ctcgggtcag ttcctatcca ttcaggaagc ggtggatgcc
      961 gcaaagaagg gcgacaccgt gctcatcagg cccggcgcct atgccgagga tgtgaccatt
     1021 cacagcaagg agaacgttcg cctgatcgga gcaggaatgg atcaggtgac gatcttgggc
     1081 cgtgagcggg tcggggtgtt ccatgtcggg aagtggccct atggcgcgac caacatcgag
     1141 atctccggca tcacgatcaa cgaacatggc gggcacgcga tggggatgtt caacggccgc
     1201 ggcatcgtgc tccaccatgt gcgggtcaaa gggatgttgt ttacgcagca ggtggaagat
     1261 gtgcgcatcg aggactgcat tatcggcggc agtgaaacca ccggcgttca attcgcaaat
     1321 tctcaggcgg tcatgcggag caatttcatc catgacaatg atcatggtgt cagtgtcgca
     1381 ggcaaatcca cggtacgact cgaacgaaac gtcattacca ggagcctgtt cgaggcggtc
     1441 atcgtcaacg atcaagcgaa ggcgaccttg ctcggcaata cctttgtgaa aaacggtggc
     1501 ggggcggcgt ttttaggcac ctcgcagaat gaagcctccg gcaatattgt gagcctcaac
     1561 gcctatgggt tcgtcgtagc cccctccagc cgggtgctgt tctcctacaa tgccatgcaa
     1621 aactccgggt ccaattatct tcggtccgga acccccaacc aaccggctcc agaactgaag
     1681 cctgattctg atttgacggt ggatccacga ttcgttgaca ctgcccggga tgatttcagg
     1741 ctccgtgcgg ataccgcgct ggtgaaaatc ggtgagttcc cctaccttgg tgccttgccc
     1801 cctctagccg gatcgcactg actgtcgtca gtaaatcgat aacaaattaa gaggttagcc
     1861 ctctgatcca gtcgaa
//
LOCUS       DAORRQ010000204         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1228_length_1862_cov_9.466519, whole genome shotgun sequence.
ACCESSION   DAORRQ010000204 DAORRQ010000000
VERSION     DAORRQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1228_length_1862_cov_9.466519"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            534..848
                     /locus_tag="PK224_21640"
     CDS             534..848
                     /locus_tag="PK224_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18408.1"
                     /translation="MKAVTTSASTSMAARPLRRDRHWLALTGPSGFAARLFRVNADRW
                     RRRARFYERHRDYFPRLASGRFVERCRELEAAYRGLAHVLFTLQPQIEWAPVRAVVSR
                     IK"
     gene            1119..>1862
                     /locus_tag="PK224_21645"
     CDS             1119..>1862
                     /locus_tag="PK224_21645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRB18409.1"
                     /translation="MRLMTILLTLSVLGASGCAKSTQPARNNRTQQALAESMPPGHLF
                     QRHMIDLNGLTLSVLEAGNGDPVIFVHGVVTTSNIFPQYVSAFSPDFRGIAVDLRGYG
                     DSEKPPSGFTIEQFSKDLITLADTLKIDRAVWVGVSMGGMILQRLALDHPERVRALVL
                     VSTTDGAMILDDDIPTIGAPRDYREVSKDMIVESFPPDAPAQTYRPLLERIPTWNATV
                     IREALTSMSQFNVHGQLSRITVPTLVMVGA"
BASE COUNT          353 a          534 c          572 g          403 t
ORIGIN      
        1 attgggcacg aagccgacgt tctgaccgcc gcgcttgagc caagcaggcc tctaggtcag
       61 ctctctggtc ttcagccatg ttccctccct gtgcagatcc ttgcatccag tgtgtgaacc
      121 caccggattg gagcacgcgc tatcaggatt atgatcgcgt ccggaaagac tttcacgggg
      181 agattgtagc tgaatccgcc atcgcctgcc ttccttccca cagtcgcggc aggcattgga
      241 cctgcgccct gtctgagggc aggcgcggga tctgtcacag ccacgtccgt agagactggg
      301 ccctccgaac ggacagaccc ataggatttc gagtcccgga attgtgaaac tgccgtagcc
      361 ggaagccccg gccagctgtg cttgtatgaa gtctttctca tgctgcacta atcatctcct
      421 catgtggcga gactacagta ggggcagggt aaaccgagac tggtgtcatc acagcgcctc
      481 tgtcacgacg ttcgaatcta tgacacaggc gaacggaaga aggaggagaa acgatgaaag
      541 cagtgaccac atccgcatcc acctcaatgg ctgcacgtcc tctcagacgg gaccggcact
      601 ggttggcact caccggtccc tccgggtttg ccgcccggtt gttcagggtc aatgcagatc
      661 gctggagacg gcgagcgcgc ttctacgagc gtcaccgcga ttactttccg cggctggcgt
      721 ccggtcgatt cgtcgagcgc tgtcgtgaat tggaggcggc gtaccggggg cttgcgcacg
      781 tcctgttcac tctgcaaccg cagatcgaat gggcgccggt ccgggcggtt gtctcgcgaa
      841 tcaagtaggt tcggatggtc gcgtggtcgg tacgttgtct gcgtgaccca ccaacgtggc
      901 gctatcggcc gtcgatgtga gggcccgacg atgggccgga tggcattcct gtgttcgtgt
      961 gcgcagggca gccactcaat gccgggaatc atcaattcga ggagtcgaag aaattgtgtg
     1021 ggagggagaa tgccgggtgg cagctcagca ttgcatctgc tcgcctgaca gcttaggctg
     1081 catgtagatt cagtgatctc tgtagaaagg ggagttgcat gcggttgatg acgatcctgc
     1141 tcacgttgag tgtcctcggc gcgtccggct gtgcgaaatc cactcagccc gctcgtaaca
     1201 accgcaccca gcaggcctta gctgagtcca tgcccccggg tcatctgttc caacgccata
     1261 tgatcgatct caatggctta accctcagcg tgctggaggc aggcaatggc gatccggtta
     1321 tctttgtcca cggcgtggtg acgaccagca atatctttcc ccaatatgtg agtgcctttt
     1381 cgccggattt cagagggatt gcggtggacc tgcgtggata cggcgattcg gagaaaccgc
     1441 cgagcgggtt cacgatcgaa cagttttcga aggatctcat caccctggcc gatacgctca
     1501 agatcgaccg tgctgtgtgg gtgggtgtgt ccatgggcgg gatgattctg cagcgtctcg
     1561 cgctggatca tccagagcgg gtccgggcgc tggtgctggt gtccacgacc gacggggcga
     1621 tgattctcga cgacgacatt ccgaccatcg gcgcgccgcg tgactatcgg gaagtgtcga
     1681 aggatatgat cgtggaaagt tttcctcccg atgcgccggc tcaaacctac aggcccttgc
     1741 ttgaacggat ccccacctgg aatgccacgg tgatccggga ggcgctgact tccatgtcgc
     1801 agttcaacgt gcatggccag ctcagccgca tcactgtgcc gactctggtt atggtcggcg
     1861 cc
//
LOCUS       DAORRQ010000205         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1266_length_1782_cov_8.925883, whole genome shotgun sequence.
ACCESSION   DAORRQ010000205 DAORRQ010000000
VERSION     DAORRQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1266_length_1782_cov_8.925883"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..446
                     /locus_tag="PK224_21650"
     CDS             174..446
                     /locus_tag="PK224_21650"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRB18410.1"
                     /translation="MRTAQEEALAKHITEWVKRGRSMNGVDVPDFSEDTDLIAAGILD
                     SRGFIEMMLEVEQQTGNRIDLNDIDPSEFTTIKGLCRCAMSQASPC"
     gene            460..1275
                     /locus_tag="PK224_21655"
     CDS             460..1275
                     /locus_tag="PK224_21655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRB18411.1"
                     /translation="MNKIVETYSRLADEYRRDEESQSCWYLAAEKASAAIELKDRYRT
                     VADIGCGTGRALCELASRGGDDRRFIGVEPADNMRALAEELARGQANTRLFNGSFEQI
                     PLESKSIDYLYSILAFHWTTNLTRSAEEVARVLKDDGDMDLFFIGRNNGHEFIRKTTP
                     VFLKYMGPAALLRSAGMRQQLTKDEAQQLFGKAFAGREVTVEESHDTYFDTLEGHWTW
                     WVRIEGQLVDIPAEKRADCDRAVKDAIATLQSPEGIPYTIHMLRVKVRNQARA"
     gene            1299..>1782
                     /locus_tag="PK224_21660"
     CDS             1299..>1782
                     /locus_tag="PK224_21660"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18412.1"
                     /translation="MKTLFIVGAGGLGREVVDIVQSSPLAAEYALAFIDDTVAPGTIV
                     HGVEVVGSRAFLRAVDPEQSAVCVAIGSPAVRRDLIGEIESWGLSLPAIVDASALIRP
                     SVVLGPGVIVGARAFVSSQSVIGAHAVINPGVLVGHDVVIGPFAVIGGGAMLSGGAKV
                     G"
BASE COUNT          366 a          474 c          592 g          350 t
ORIGIN      
        1 tttatcgccg gagacgccgc gcaggatgtc gggcgtatcc tgcaacattg ccggcgggtt
       61 ctgcccgatt acatggtgcc gagccgcatc aggtttctgg aacacatgcc gttgaacgtc
      121 catgggaaaa cggatcggtt gaaactacgg gatctattga aggaggaaca gcaatgagaa
      181 ctgctcagga agaagcgttg gcgaaacaca tcacggagtg ggtcaagcgg ggccggagca
      241 tgaacggcgt ggacgtgccg gacttttcag aggacacgga tctgattgcg gccgggattc
      301 tggattcgcg gggattcatc gaaatgatgc tcgaggttga gcaacagaca ggaaatcgaa
      361 ttgatttgaa cgacatagac ccgagtgaat tcaccacgat caagggcctc tgtcgttgtg
      421 ccatgtcgca agcttcaccc tgttgaaagg aatacactca tgaataaaat cgtcgaaaca
      481 tactcgcgcc tggcggatga ataccgtcga gacgaggagt cgcaatcctg ttggtatctg
      541 gctgcggaaa aggcgtcggc tgcgatcgaa ctcaaggatc ggtaccgcac ggtcgccgat
      601 atcggctgcg ggaccggtcg ggccctctgc gagttggcgt cccgcggagg ggacgaccgc
      661 cgtttcatcg gtgtagaacc ggccgacaac atgcgagccc tggccgagga attggcgcgc
      721 gggcaggcca acacccgcct gttcaacggg tcgtttgagc aaattccgct ggagtcgaag
      781 agcatcgatt atctctacag catcttggcg tttcattgga cgacgaacct cacccgctcg
      841 gcggaggagg tggcgcgagt gctgaaggac gacggcgaca tggatctgtt cttcatcggc
      901 cgcaacaacg gccacgaatt catccgcaag acgactcctg tgtttctcaa atacatggga
      961 ccggcggcgc tgttgcggtc ggcggggatg cgccagcagt tgacaaagga tgaagcacag
     1021 cagctgttcg gcaaggcctt tgcgggacga gaggtgacgg ttgaggagtc ccacgacacc
     1081 tacttcgata ccctggaggg ccattggacg tggtgggtgc gcatcgaggg ccagttggtc
     1141 gatattccgg ccgagaagcg ggccgattgc gaccgcgccg tgaaagatgc gatcgccacc
     1201 cttcagagtc ctgaaggcat tccctacacg atccacatgt tgcgcgtgaa agtccgtaac
     1261 caggcccgcg cgtaggtcca gagattggat acatgaccat gaaaacactc ttcatcgtgg
     1321 gcgcaggcgg attaggaagg gaagtcgtgg acatcgtcca gtcgtctccc ctcgcagcgg
     1381 agtatgcgct ggcatttatc gatgacaccg ttgcgccagg gaccatcgtg catggggtcg
     1441 aggtcgtcgg ctctcgggcc ttcctgagag ctgtcgatcc cgagcaatcg gcggtgtgcg
     1501 ttgccatcgg cagtccggcg gtccgccgcg acctgatcgg agaaatcgaa agctgggggt
     1561 tgtcgctccc ggcgatcgtg gatgcttcgg ccctgatccg accgtcggtg gttctgggcc
     1621 ccggcgtcat tgtcggggca cgggcctttg tgagttccca gagcgtgatc ggcgcccacg
     1681 ccgtgatcaa tccgggggtg ctcgtagggc atgacgtcgt gatcggaccg tttgccgtca
     1741 tcgggggagg cgcgatgttg tccggtgggg ccaaggtggg gg
//
LOCUS       DAORRQ010000206         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1318_length_1701_cov_6.057716, whole genome shotgun sequence.
ACCESSION   DAORRQ010000206 DAORRQ010000000
VERSION     DAORRQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1318_length_1701_cov_6.057716"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1701)
                     /gene="carB"
                     /locus_tag="PK224_21665"
     CDS             complement(<1..>1701)
                     /gene="carB"
                     /locus_tag="PK224_21665"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRB18413.1"
                     /translation="IEQSVIGWKEFELEVMRDLKDNVVIICPIENLDPMGVHTGDSIT
                     VAPALTLTDKEYQMLRDAAVRIIREIGVDTGGANIQFGMNPANGEMVVIEMNPRVSRS
                     SALASKATGFPIAKIAAKLAVGYTLDEITNDITGVTKASFEPTIDYVVVKIPRFAFQK
                     FPGADPTLTTQMKSVGEVMAIGRTFKESLQKAIRSMEVDQFGFSSKMGLDLAVPPGLD
                     REEAMEQVRKAVRTPLPDRLWRLADGMRLGMPNQELFALTKIDPWFLQQIRELIEFEA
                     RLVAERATLGTTGLRPDLLIEAKELGFSDPRLAQLLGVEQQTVRGWRLALSQGAQPRS
                     VTYKRVDTCAAEFEAHTPYLYSTYERECEARPTDKKKVVILGGGPNRIGQGIEFDYCC
                     VHAAMALREEQIETIMVNCNPETVSTDYDTSDRLYFEPLTEEDVLNIVEREQPMGVVL
                     QFGGQTPLKLALSLSRAGVRILGTSPDAIDRAEDRARFRELLDKLGLRQAESGMAHSV
                     EEALTIAAEITYPVMVRPSYVLGGRSMQIVYDEPGLLHYMNTAVKASEKHPVLIDKYL
                     R"
BASE COUNT          311 a          512 c          501 g          377 t
ORIGIN      
        1 cgcaaatact tatcgatcaa caccggatgt ttctccgatg ccttgaccgc cgtgttcatg
       61 tagtggagca gtcccggctc gtcatagacg atttgcatcg accgtccgcc gagcacatac
      121 gacggccgca ccattacggg ataggtgatc tcggcggcga tcgtcaacgc ttcttcgacg
      181 gagtgggcca tcccgctttc ggcctgacgc aacccgagct tgtcgagtaa ctcacggaaa
      241 cgggcgcgat cctctgcgcg gtcgattgca tccggactgg tgccgagaat cctgacaccg
      301 gcgcgggaca gggagagcgc cagtttcaag ggcgtctgtc ccccgaattg cagcaccaca
      361 cccatcggtt gttcgcgttc cacgatgttg agcacgtctt cttccgtcaa cggttcgaag
      421 tagagacggt cggaggtgtc atagtccgtg ctgaccgttt ccggattgca gttgaccatg
      481 atggtttcaa tctgctcttc ccgcagcgcc atggcggcgt gcacacagca gtagtcgaac
      541 tcgatcccct gcccgatccg gttcggtccg ccgccgagaa tgacgacttt ctttttgtcg
      601 gtcggccggg cttcacattc ccgctcgtag gtcgagtaga gatacggcgt atgggcttca
      661 aattcggccg cacaggtatc gacccgctta taggtcacac ttcgcggttg agccccttgg
      721 ctcaatgcca gccgccagcc gcgaacggtc tgttgttcca ctcccaggag ttgagccaga
      781 cgtgggtcgg agaagcccag ttccttggcc tcgatcagga gatcaggccg cagacctgtg
      841 gtgccgagtg tcgcccgttc cgcgacgagt cgggcctcga attcgatcag ttcacgaatc
      901 tgttgcagaa accagggatc gatcttggtg agcgcgaata actcctgatt gggcatgccg
      961 agccgcatgc cgtcggccag ccgccagagc cggtcgggca acggggtgcg taccgccttg
     1021 cggacctgct ccatggcctc ctcccggtcg agaccgggtg gcaccgcgag atccagcccc
     1081 atcttcgagc tgaatccgaa ctgatcgacc tccatggacc ggatcgcttt ctgcagcgat
     1141 tccttgaagg ttcgtccgat ggccatcact tcgcccaccg acttcatctg tgtggtcagg
     1201 gtgggatcgg ctccgggaaa tttctgaaag gcaaagcgcg gaatcttgac gacgacatag
     1261 tcgatggtcg gctcgaacga ggctttcgtc acaccggtaa tgtcgttcgt gatttcgtcg
     1321 agggtatagc cgacggccag cttggccgcg atcttggcga taggaaatcc cgtcgctttc
     1381 gatgccaggg ccgagcttct ggagacacgc ggattcatct cgatgacaac catttcgccg
     1441 ttggcggggt tcatgccgaa ctggatgttg gctccgccgg tgtcgacgcc gatctcacga
     1501 atgatccgca cggccgcatc gcgcagcatt tgatattctt tgtcggtcag ggtgagtgcc
     1561 ggggccaccg taatactatc gccggtgtgc acccccatcg gatcgaggtt ctcgatcggg
     1621 cagatgatga ccacgttgtc cttcaggtcg cgcatcactt cgagttcgaa ctctttccat
     1681 ccgatgacgg actgctcgat g
//
LOCUS       DAORRQ010000207         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1319_length_1699_cov_33.066910, whole genome shotgun sequence.
ACCESSION   DAORRQ010000207 DAORRQ010000000
VERSION     DAORRQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1319_length_1699_cov_33.066910"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK224_21670"
     CDS             <1..231
                     /locus_tag="PK224_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18414.1"
                     /translation="ENGDEDALETLDRLAFSRVGLTLRGDQALLYVANQRPNATGAGF
                     LFWFVRRQGSWEIYDTDVLWVAQPDQNPSGRR"
     gene            282..911
                     /locus_tag="PK224_21675"
     CDS             282..911
                     /locus_tag="PK224_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRB18415.1"
                     /translation="MIRKTFSVPPLGCNCSIIGDPITKQAIVVDPGGAPERILQEVRA
                     MGLTVVSIVHTHAHFDHFLASGAMKQATGAALCLHPDDLPLWDILEMQCRMFGVPYVP
                     APPPDYWLKDEERLAIGELEGIALHTPGHTPGSMCFHFPAAKLVVAGDTLFRGSIGRT
                     DLWGGDFDVIEQSIRERLYTLDEQTAVVTGHGPETEIGRERETNSFVRA"
     gene            1162..1677
                     /locus_tag="PK224_21680"
     CDS             1162..1677
                     /locus_tag="PK224_21680"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRB18416.1"
                     /translation="MSATTRFWLVFPLLSLVLALSVSDPQARAGEAGSSPNVPISDVR
                     VRLSDHGPVVLLSADGKTIPVFVDHTVAASIQAALTGEKLPRPLSHDLMHTILESLGG
                     RVVRTVITLKAGTYYGSLTVAFQGQEKVFDSRSSDSIALAIHFQAPILVGRDMLDAVG
                     TSIGEPKPEAL"
BASE COUNT          293 a          482 c          541 g          383 t
ORIGIN      
        1 gagaatggtg acgaggatgc tctcgagacc ctcgaccggc tggccttctc ccgtgtggga
       61 ctgacattgc ggggcgacca ggccttgctc tatgtggcga atcagcggcc taatgcgacc
      121 ggtgccggat ttttgttctg gttcgtgcgc cgacagggga gttgggagat ttacgatacg
      181 gacgtgttgt gggttgcgca gccggaccag aatccttcag gccgtcgata gcaggggggc
      241 aggtgcgtcg tggtactcgt ttggttcact gaggagtgtg catgattcgc aagacctttt
      301 ccgttccgcc gctcggttgt aactgctcga tcatcggaga tccgatcacg aaacaagcga
      361 ttgtggtcga tccgggcggg gcgcccgagc gcattctgca ggaagtccgc gcgatggggt
      421 tgacggtggt gagcattgtg cacacccatg cgcatttcga tcactttctc gcatcggggg
      481 cgatgaagca ggcgaccggc gccgcgctct gcctgcatcc cgatgatctt cctctgtggg
      541 acattctgga gatgcagtgc cgcatgttcg gcgtgcctta tgtcccggcg ccaccgccgg
      601 actattggtt gaaggatgag gagcggttgg cgatcggcga gttggaaggg atcgcgttgc
      661 acacgcccgg gcataccccc ggttccatgt gttttcactt tcctgctgcg aagctggtgg
      721 tggccggtga caccttgttt cgcggatcca tcggccgcac cgatctctgg ggcggagatt
      781 ttgatgtcat cgaacagtcg atccgggaac gactgtacac gctcgatgaa cagactgccg
      841 tggtcactgg gcacgggccc gagacggaga ttggcaggga gcgggagacc aattcctttg
      901 tgcgagccta gcaggatgcg caaaaagtcc accagcggcg ttctcgcttc gttcagagga
      961 tcaccggacg gcgcagagta cgattcacct cttcgctcgc tgcggcctcg ctggacggcc
     1021 tttttgcgca tcctgctggc gattctgacg ctgccatggc atgcgagctg tccgggatgt
     1081 attacgcaag aattgacttt tccacagttt ggtagcagcg ggggctcata gcagcttcca
     1141 tgtgaaccac aggagggaac catgagcgcg acgacacgat tttggttggt gtttccgctc
     1201 ctctcactgg tgctggcgtt gtccgtttcc gatccccaag cccgtgccgg cgaggccggc
     1261 agttctccca atgtgcccat cagcgatgtg cgtgtgcggc tctccgatca cggccccgtg
     1321 gtcctgctct cggcggacgg caaaaccatt cccgtctttg tcgatcacac ggtggccgcc
     1381 tccattcaag cggccttgac cggtgagaaa ttgccccgcc cgctttccca cgacctgatg
     1441 cataccattc tcgagtcgct cggtggtcgt gtggtccgga ccgtgattac cctcaaagcg
     1501 ggaacgtatt acggaagttt gactgtggcg ttccaggggc aggaaaaggt cttcgacagc
     1561 cggtcctcag actccattgc cttggcgatt cacttccagg cgcccattct cgtgggacga
     1621 gatatgttgg atgcggtcgg tacgtcgata ggggaaccca agccggaagc cctgtagcgt
     1681 cgcgaagcct gcctgcacg
//
LOCUS       DAORRQ010000208         1655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1347_length_1655_cov_41.313750, whole genome shotgun sequence.
ACCESSION   DAORRQ010000208 DAORRQ010000000
VERSION     DAORRQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1347_length_1655_cov_41.313750"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..>1655)
                     /locus_tag="PK224_21685"
     CDS             complement(136..>1655)
                     /locus_tag="PK224_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRB18417.1"
                     /translation="FEDGLTAQKAITMAGGFTEKAAKAVLSITRRTANQEGTLSAQLD
                     TALLPDDLIVVGQLQKFYLMGEVTRPGQYPHEDLLTVKKAVSLAGGFTEKADKLALKV
                     TRVTESGAQTLAIGQDELVLPSDILAVGSHNHKVYISGEVRTPGAYPYSENISIQKAL
                     TLAGGVTEKADREDLTVRRIVDGQERTLVLKLDALVEPEDLIVVPEGQKVYVTGEVKT
                     PGRYAYESGATVQRVLTLAGGVTEKAEASVVKVTRMKEQGVETAVLPQDALVFPEDII
                     LVVPVTAKFYVSGEVKTPGSYAYKEGLSLQKALAMAGGLTDKGDHDRLEIVRMANNRE
                     ERSVVTLETPVLPDDTIVVSERQKFYVTGEVKTPGRYFYESGISMQKAITMAGGFTEK
                     ADKADLRVERRQDKAVAVTSADPNALVLPDDLIVVPQARRFYVNGEVKKPGDYWYERG
                     LTLHMAITMAGGFTDKASKAPKVLRKVNGQERTVELALDAPVLPDDIIVVAQRFF"
BASE COUNT          324 a          547 c          420 g          364 t
ORIGIN      
        1 cgccagcgca cagctcagcc agcaccagaa aatccgatta tgcatgggat gcctttgcct
       61 ccacgttcaa cgacacaagg gatgggcact cattgcgccc gcctatcgag ggcccagcac
      121 gagtgcacgg atgggctaaa agaaccgttg cgcgacgacg ataatatcgt cgggcaggac
      181 cggggcatcc aaggccaatt ccactgtgcg ctcctgacca ttcaccttcc gcaacacctt
      241 cggcgccttg gaggccttgt cggtaaagcc gcccgccatc gtaatggcca tgtgaagggt
      301 tagcccccgt tcataccaat agtcgcccgg cttcttcact tctccgttga cgtagaatcg
      361 ccgcgcttgc ggtaccacga tgaggtcatc gggcagcacg agtgcgttgg gatcggccga
      421 cgtgacggcc acggccttgt cctggcgccg ttccaccctg agatccgcct tgtctgcttt
      481 ctcggtaaat cccccggcca tcgtgatggc cttctgcatg gagataccgg actcatagaa
      541 atatcggccc ggagtcttga cctcgcccgt cacgtagaat ttctgacgct cggaaacgac
      601 aatggtatca tcgggcagca ccggagtctc cagcgttacc accgaacgtt cttcccggtt
      661 gttcgccatt cgaacaattt ctaaacgatc atgatccccc ttgtctgtca gccctcccgc
      721 catggccagc gctttctgca aactcaggcc ttccttgtag gcatagctgc caggcgtttt
      781 cacttcgcca ctgacataga acttagctgt cacggggaca acgagaataa tgtcctccgg
      841 aaatacgagg gcatcctgcg gcaacacggc cgtttccacg ccctgttcct tcatcctggt
      901 gactttgacg acgctggcct ccgctttttc cgtcaccccc ccggccaacg tcaagacccg
      961 ctgaaccgtc gcacccgatt cgtaggcata ccgccccggc gtcttgacct cgcccgtcac
     1021 atacaccttc tgaccttcag gaacgacaat cagatcttcc ggctctacca gggcatcgag
     1081 tttcaggacc agcgtccgtt cctggccatc aacgatacgc ctgaccgtga ggtcctcgcg
     1141 gtcggccttc tccgtgacgc ccccggctaa ggttaaggcc ttttggatac tgatgttttc
     1201 actgtagggg taggcaccgg gagtgcgcac ctcaccgctg atatacacct tgtgattgtg
     1261 actacccacc gccaagatgt cgctcggtaa gactaactca tcttgcccga ttgcgagcgt
     1321 ctgcgcgccg gactccgtaa cccgcgtgac cttaagggcc agcttatcgg ctttctccgt
     1381 gaaccctccg gccaacgaga cggccttctt tacagtcaac aagtcctcat gggggtattg
     1441 gccgggccgc gtcacctcac ccatgagata aaatttctgc aactgcccca cgacaatcag
     1501 gtcgtcaggc aggagtgccg tatccagctg agcggatagg gtgccttcct gattggccgt
     1561 acgccgtgtg atcgacagca cggctttcgc cgccttttcc gtgaaccctc cggccatggt
     1621 aatggccttc tgcgccgtca acccatcttc aaacg
//
LOCUS       DAORRQ010000209         1626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1364_length_1626_cov_5.810948, whole genome shotgun sequence.
ACCESSION   DAORRQ010000209 DAORRQ010000000
VERSION     DAORRQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_1626_cov_5.810948"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1147..>1626
                     /locus_tag="PK224_21690"
     CDS             1147..>1626
                     /locus_tag="PK224_21690"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HRB18418.1"
                     /translation="MTYQRAREALDRALIDTRSTASIEQTGDFQHLPPAVILDLDETV
                     LDNSPFEARLIAQRTTFNQPMWEQWVQEASAQAVPGVLEFIAEARKKGVALFFVSNRG
                     AHQESSTRRNLEKLGIHLPIDPDALLLEGEPPFRWPPNKSSRRRYLAERYRILLLIGD
                     "
BASE COUNT          377 a          493 c          432 g          324 t
ORIGIN      
        1 ttttcaacct gggcctgaaa gatagaactc tctcccacaa gcgaatcgat cctcacgcgc
       61 tgagaccgac cgataccatg tggtgttgag ctccgtccgg ctacgaggac tcgcccgatt
      121 cgtagggcca attcgtttcc atcgacaggg cacaagacaa aatcattcaa ctccgccgga
      181 actccgttca ggagctgtgg ccggcggcca tgctccgctg caaacaatcc tagtatcggc
      241 acatccggcc aacgaccggc cacatcggtc agcctctgcg ccgataaacg atcagtagat
      301 ggatggtgaa acaacagcac gtgcggagag cgcggaagcg ttgcggaagg atccgacacc
      361 cggtggaact ctatggtccc tccaggcagt gcacgttgca aaacgtttac aaggtgagac
      421 tcatcgaacc aggcatgata ctgattgtcc gaagtgacca agaggattct gatgaagtcc
      481 ttcatccgac tcaccctttg ctgcgagaag cttatctacg ttcagccggc gaccggccgg
      541 cagacacaaa ccgcctttct acgtaaatgt gcaccattcg taccatccga tgtgcgcagt
      601 gcaacgcatg tatcagaacg agacaccgtc gacaaacccc gctcgatata catattctga
      661 atgcaccggg ttgcgatgac gagtggcttc aaccgcagat gaccatactg ctggcatctt
      721 catgtatgtg attcgataca gtccctcgtg attttggata cagtccgcac caccggtcag
      781 catcgcccgc tcttataaca caggacgccg aacctgacca tgcacccgca ggatagaccg
      841 ctcgattctc cacgcgtaga atgcacgctc gcacgggtga tgaacagttc gacgccatct
      901 acgtattccc aagcgtcttt agcacgacag cggagaaccg catggcctat gacagaggat
      961 ccagagatgt cgcacctagc acggggcact agcgcaggag gagctttcgg acagcatcgg
     1021 tcctggtcct tcctacttat gctcctctta ttttgtggag gctgttcgga ggtccgggac
     1081 agtcatgaac tgctgcacag cctgctctgg atgcgcacgt ctgctgagta ccatgtcctc
     1141 agtgtcatga cctatcaacg agcgagggag gcgctggatc gcgcgttgat cgacacgcgc
     1201 tcaacagcca gtatcgaaca gaccggagac tttcaacacc tgcctcccgc cgtgatcctg
     1261 gatctcgacg aaacagtgct ggacaacagc cccttcgagg cgcggttgat tgcacagcgc
     1321 accactttca atcagccgat gtgggaacaa tgggtacagg aagcctccgc gcaagcggtg
     1381 ccgggagtcc tggaatttat cgccgaggct cgcaagaaag gggtcgcctt gttcttcgtc
     1441 tcgaaccgcg gggctcacca ggaatcctca accaggcgca acctcgagaa actcggcatt
     1501 caccttccga tcgatcccga tgccctgctg ctcgaaggtg aacctccgtt ccggtggccg
     1561 ccgaacaaat cgagccggag gcgatacctg gccgagcggt accggatcct gctcctcatc
     1621 ggggac
//
LOCUS       DAORRQ010000210         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1400_length_1563_cov_4.598806, whole genome shotgun sequence.
ACCESSION   DAORRQ010000210 DAORRQ010000000
VERSION     DAORRQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_1563_cov_4.598806"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>1095)
                     /locus_tag="PK224_21695"
     CDS             complement(109..>1095)
                     /locus_tag="PK224_21695"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HRB18419.1"
                     /translation="LSKQGDWGDVMESLTNMLAQHGALVLFAVVFAEQVGLPFPALPL
                     LVAGGVLVGTGHLAWSSALGAAILATLMADAIWYLAGQWRGRPVLSLLCRIALEPDAC
                     VRRTEDFFLRHGPKSLIVAKFIPGLSTIAPPLAGIVGLSVPLFLLYDTLGALLWAGSG
                     LEFGYAFSGQLEQAFAYSEQVMPALLLTMVFLLAGFVSLKAWHRHRQLRLVPRMTVAE
                     LAEKLTAAEPPVLIDVRPRARAEAEPGIPTAVLMSLDELARRYQEFPRHRDVVVYCGC
                     PADVASAQGALLLQKKGFTRVWPLAGGIEAWRATVTQNAVTERALERQTVAA"
     assembly_gap    1096..1105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1127..1417)
                     /locus_tag="PK224_21700"
     CDS             complement(1127..1417)
                     /locus_tag="PK224_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18420.1"
                     /translation="MSSIKNRRFVSLATAATSSRANCRHGQADRVAGPVRTVVDDSGS
                     LAITAWTCAACGNLIEELHLLSRDGTARPYPIRHAVVPLVQTRRLAALPVWR"
BASE COUNT          338 a          507 c          448 g          260 t
ORIGIN      
        1 ggccacggtt ttcatcgcac gcagtcccca ataggcctgt tccggggtcg gtattttcaa
       61 ttccatcttc acctccctga acctgttgtc agcctgtcat tccaccggtc aggctgcaac
      121 agtctggcgt tcaagagccc tctccgtaac tgcgttctga gtcacagttg cccgccaggc
      181 ctcgatgcca ccggccagcg gccacacgcg agtgaacccc ttcttctgta aaaggagcgc
      241 accttgagcg ctggccacat ccgccggaca cccgcagtag acgaccacgt cgcgatggcg
      301 cgggaattcc tgataacgcc gggccaattc atccaacgac atcagcaccg ccgtgggaat
      361 gcccggttcc gcctccgccc tcgcgcgcgg tcggacatcg atcagcaccg gcggttcagc
      421 cgccgtcagc ttctccgcga gctctgccac cgtcatgcgc ggcaccaggc gaagctgccg
      481 gtgccgatgc caggctttaa gggatacgaa gccggccagc agaaacacca ttgtgagcag
      541 caaggcaggc atcacctgct ccgaataggc gaaggcctgc tcgagctgac cgctgaacgc
      601 atagccaaac tccaatccag atcccgccca taacaacgca ccaagggtgt catagagcag
      661 gaacaggggc acactcagcc cgacgatgcc ggcgagcgga ggggcgatcg tactcaaccc
      721 gggaatgaat ttcgccacga tcaacgactt cggtccgtga cgcagaaaaa agtcctccgt
      781 gcgccggacg caggcatccg gttccaacgc gatccggcag agcagactga ggactggacg
      841 gccgcgccat tgtcccgcca ggtaccaaat cgcgtccgcc atcagcgtgg caaggatcgc
      901 cgcaccgagc gcactgctcc aggccagatg gccggtgccg accaacaccc ctcccgccac
      961 cagtagcggc agcgccggga agggcagtcc gacttgctcg gcaaacacaa cggcgaacag
     1021 gacgagtgct ccgtgctgag ccaacatgtt cgttaaggat tccatcacgt caccccaatc
     1081 tccctgtttc gacaannnnn nnnnncgtca tgaccatacc cgcggcttac ctccagaccg
     1141 gcaaagcagc aagccgcctg gtttgcacca ggggaacaac ggcatggcga attggatagg
     1201 gccgagccgt cccatcccgc gagaggagat ggagttcctc aatgaggttg ccgcaggcgg
     1261 cgcaggtcca agcggtgatt gccagtgatc cactgtcgtc gaccacggtg cgaaccggtc
     1321 ccgcgactcg atcggcctgt ccgtggcggc agttcgcccg actcgacgtt gcagcagtag
     1381 ccagggagac aaaccgtcgg ttctttatgg atgacatgga acgctcctct catcgcattg
     1441 aaatgaagac tgcggtcatg ctcggatatc agtcggagaa agcatggacc gttccagcga
     1501 tgtggaactc gtaacttgca gagagactgc ttgcaggacg gggagttacg cagtgaggag
     1561 cga
//
LOCUS       DAORRQ010000211         1515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1442_length_1515_cov_4.080137, whole genome shotgun sequence.
ACCESSION   DAORRQ010000211 DAORRQ010000000
VERSION     DAORRQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_1515_cov_4.080137"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            504..1064
                     /gene="pyrR"
                     /locus_tag="PK224_21705"
     CDS             504..1064
                     /gene="pyrR"
                     /locus_tag="PK224_21705"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRB18421.1"
                     /translation="MTTEPTTGNRQERVVMDAGDIARAVTRIAHEILERNKGIKDLAL
                     VGIRTGGVHLAHRLVRRIHDIEGTQIPIGELDITLYRDDLSLRKDQPILRKTSVPFKI
                     SDLKVVLVDDVLFTGRTIRAAMDSLIDLGRPAEIQLAVLVDRGHRQLPIKANYIGKNI
                     PTSREEAIEVHLEENGEEDRVVILRA"
     gene            1079..>1515
                     /locus_tag="PK224_21710"
     CDS             1079..>1515
                     /locus_tag="PK224_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRB18422.1"
                     /translation="MGLKRKDLLSLTDLSADDITLILETADSFKEVSGREIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLARGVKSSVINASDGWHEHPTQALLDLYT"
BASE COUNT          350 a          453 c          442 g          270 t
ORIGIN      
        1 catcaccgaa aagaacagga ccgcgacgat gtctgcggcc gaacaacagc tggagggatg
       61 tccgctcccg gcttcagtcg tcgccttcac actgtccatg cgaaggtgcg tagccttatt
      121 ggccaaagcg atcagaaggt caggcgtggc ggtagaggca gcagaggcag tcatgcgcga
      181 tcctttcagg atgggagctg ataaattccg ggtgactgga tgatagtcga gagaggcgga
      241 ccgcctctca acggcacgaa gctaacaaat ggcatgcagg gagtcaatga aggcgtcgtc
      301 gtgcaacaca ggccactcgg ggcacagaat tcttgacacg gtaggggcaa ctcgttaaga
      361 tcggctcgcc tttaacgctc atcctgtgag gtgggtaagg agatcatgac cacacgacac
      421 ggcagccagc cgtccgacag cccgcacctt ctcatccaaa ccggtgggaa ggttttttta
      481 tgcccccatc gactgaacca cctatgacaa ccgaacccac aacaggcaac cggcaggagc
      541 gtgtggtgat ggacgccggc gatatcgccc gcgccgtgac ccgtatcgcg catgaaattc
      601 tggaacgcaa caagggcatc aaggacctgg ccttggtggg catccggacg gggggcgtgc
      661 acctggcgca tcgtctcgtg cggcgcatcc acgacatcga gggcacccag atcccgatcg
      721 gcgaattgga catcacgctc tatcgtgacg acctgtcgct ccgcaaggat cagccgatcc
      781 tccgcaagac ctcggtgccg ttcaaaattt ccgatctcaa ggtggtcctc gtggacgatg
      841 tgctcttcac cggccgcacc attcgcgccg ccatggatag cctgatcgac ctgggacgac
      901 cggcggaaat tcagctggcg gtactggtcg atcgcggcca tcggcaactg cccatcaaag
      961 ccaattacat cggcaaaaat attcccacgt cacgagaaga ggccattgag gtccatctcg
     1021 aagaaaacgg ggaagaggat cgcgtcgtca tcctcagggc gtaaccggag acattgtcgt
     1081 ggggctcaaa cggaaagacc tgctcagcct gaccgacctg tcggcggacg acatcacgtt
     1141 gatcctggaa accgcggatt ccttcaagga agtctccggc cgggagatca aaaaggtgcc
     1201 ggcgttacga ggcaagacgg tcgtcaacct cttcttcgag ccgagcacca gaaccagaac
     1261 gtcgtttgaa ttggccgcca agcgtctcag cgccgatgtc atcaattttt ccccctcttc
     1321 cagcagcgtg gtgaaggggg aaacgctcct ggatacggcc cgcaacatcg aagccatgca
     1381 ggccgatatc atcgtcctgc ggcactcctc agccggcgcc gcagagaccc tggcgcgggg
     1441 cgtcaagtct tcggtcatca atgccagtga cgggtggcat gaacatccga cacaggctct
     1501 gctcgacctg tacac
//
LOCUS       DAORRQ010000212         1499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1455_length_1499_cov_6.603878, whole genome shotgun sequence.
ACCESSION   DAORRQ010000212 DAORRQ010000000
VERSION     DAORRQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1455_length_1499_cov_6.603878"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>216)
                     /locus_tag="PK224_21715"
     CDS             complement(<1..>216)
                     /locus_tag="PK224_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18423.1"
                     /translation="TGPTIAPTLITRHTLAEQRARPRILVAEDNVVNQKLAVRMLDRL
                     GYQPDIVSNGQEAVAAFERGLYAAIVMD"
     assembly_gap    217..226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<227..>1499)
                     /locus_tag="PK224_21720"
     CDS             complement(<227..>1499)
                     /locus_tag="PK224_21720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRB18424.1"
                     /translation="AFAIDLSEVHATHRALNETADRLRESNRQLDQALQQAQTAARAK
                     SSFLAMITHELRTPMNGVIGMASLLLETPLTEEQRSFTNTIEQCGEAQLSLINDVLEC
                     SKIEAGKLELEHLDFHLRTTVEDVLSQFAERAQRKGLEITGLVHAAVPNALRGDPGRL
                     RQVLTNFVGNAIKFTERGEVTLQAFLERDSPSEVIIKFEVTDSGIGISEDVQSRLFQA
                     FTQADSSTTRKYGGTGLGLAISKQLVELMGGQVGLRSQPGQGTTFWCTARFQKQAVCA
                     PAIVPSVELSGRRILIVDDNESNRTILHHLVSGWGMQDGQARHADEAIQMLEQAAAQG
                     RPYDAAVLDMLMPGKDGLQLAQDIRTHPQGAGVRLIVLTSLIQPGHAERARRAGFTAY
                     LTKPVRHDQLQGCLRVVFGLQGSATAAVPGVE"
BASE COUNT          284 a          453 c          440 g          312 t
ORIGIN      
        1 gtccatgacg atcgcggcat agagaccccg ttcaaacgcc gccacggctt cctggccgtt
       61 ggacacgatg tccggctgat agccgaggcg gtccaacata cggacggcca gcttctggtt
      121 caccacgttg tcttctgcga caaggatacg cgggcgggca cgctgttcgg ccaaggtgtg
      181 gcgggtgatc aatgtcgggg caatcgtcgg tcccgtnnnn nnnnnnctcc acccctggaa
      241 cggcggcggt ggccgatccc tgcagtccaa acacgacgcg gaggcaacct tggagctggt
      301 catggcgtac aggcttcgtc agataggcgg taaaacctgc gcgccgcgct cgttcggcat
      361 gaccgggctg aatcagggag gtcaagacaa tcaagcgaac cccggctcct tgagggtggg
      421 tcctgatatc ctgggccagt tggagtccat ctttccccgg catcagcata tcgagcaccg
      481 ccgcatcgta gggccggcct tgtgcggcgg cctgttccag catctgaatg gcttcatcgg
      541 catggcgcgc ctggccgtcc tgcatacccc agcctgatac cagatgatga agaattgtgc
      601 gattggactc attatcgtcg acgatcagaa tgcggcgacc gctcaactcg accgagggaa
      661 cgatggccgg cgcacagacc gcctgcttct gaaatctcgc cgtgcaccag aacgtggtgc
      721 cctggcctgg ttggctgcgc agcccgacct gaccacccat gagttccact aattgtttgg
      781 agatggcgag ccccagtccc gtccccccgt attttctggt ggtggaactg tcggcttggg
      841 tgaaggcctg gaacagccgt gactgcacgt cttcgctgat gccgatgccg gagtccgtca
      901 cttcgaactt gatgatgacc tcggaagggg agtcgcgctc cagaaaggcc tgcagcgtga
      961 cttctcctcg ctcggtgaac ttgatggcat tgccgacgaa attcgtcagc acctgacgca
     1021 gccgtccggg gtcgcctcgt aatgcgttcg gcacggcggc atggacgagg cccgtaatct
     1081 ccaggccttt acgctgagcc cgttctgcga attgagacaa cacgtcttca acggtcgtac
     1141 gtagatgaaa atcgaggtgt tccaattcca acttgcccgc ctcgatcttg ctgcattcca
     1201 gcacatcgtt gatgagcgaa agttgcgctt ctccgcattg ctcaatggta ttcgtgaaag
     1261 accgttgctc ttcggtcaac ggtgtctcca gcaacaaact cgccatcccg atcacgccgt
     1321 tcatcggcgt gcgcaattcg tgcgtgatca tcgctagaaa cgacgacttc gcccgtgccg
     1381 cggtctgtgc ctgctgaagg gcctgatcca actggcggtt actctcgcga agacgatcag
     1441 cggtctcgtt gagcgcccgg tgggtggcat gcacttcact cagatcaatg gcaaaagcc
//
LOCUS       DAORRQ010000213         1436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1501_length_1436_cov_15.985518, whole genome shotgun sequence.
ACCESSION   DAORRQ010000213 DAORRQ010000000
VERSION     DAORRQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1501_length_1436_cov_15.985518"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK224_21725"
     CDS             <1..399
                     /locus_tag="PK224_21725"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRB18425.1"
                     /translation="QHIRLAGQGAPGIKGTSPGDLYLEIHFHPHRLYQVQGRDLSLSL
                     PIAPWEAALGATAKAPTPSGVVDVKIPPGSRNGRKLRLKGRGIPGEPAGDLYLILEVV
                     LPPADNERAQAIYRTMAQELAFNPRQALGV"
     gene            400..744
                     /locus_tag="PK224_21730"
     CDS             400..744
                     /locus_tag="PK224_21730"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRB18426.1"
                     /translation="MEKPQDILTGTVIGDESVLSINELARACGAESQWIIELVAVGVL
                     EPEGPETSNWRFHAADLSCARRVARLQRDFDASLDAAAAMLDLIGQIEQLRARLKQAG
                     LDADEAFGGKDT"
     gene            878..>1436
                     /locus_tag="PK224_21735"
     CDS             878..>1436
                     /locus_tag="PK224_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRB18427.1"
                     /translation="MTTSMTRERGLELLRHMIRIRRFEERTAELYQLGKIHGFLHLYI
                     GEEAVAAGSIPSFTNEDAIVATYREHGHALVRGTSMNQLMAELYGKAAGCARGRGGSM
                     HFFDASRRFYGGLAIVAGGLPVAVGLALADKMQGRTRVTGCYFGDGAVAEGEFHESLN
                     LAALWKLPVLFLCENNLYAMGTALAR"
BASE COUNT          275 a          443 c          469 g          249 t
ORIGIN      
        1 cagcacatac ggctggccgg gcaaggtgct ccggggatca aggggacctc tcccggcgac
       61 ctctacctcg aaattcactt tcatccccac aggctctatc aggtgcaggg ccgggacctg
      121 tcactctcgt tgccgatcgc cccttgggaa gcggcgcttg gcgcaacggc caaggcaccc
      181 actccgagcg gagtggtcga cgtaaaaatc ccgccggggt cgcggaacgg acgcaaactc
      241 aggctcaagg ggcgcggcat tcccggcgaa ccggcggggg acctgtatct catccttgag
      301 gtggtgcttc cgccggcgga caacgagcgc gcgcaggcaa tctatcgcac catggcgcag
      361 gagctggcat tcaatcctcg ccaggcactg ggggtctgaa tggagaagcc acaggatatt
      421 ctcacaggaa cggtgatcgg ggacgaaagc gtgttgtcga tcaatgagtt ggccagggct
      481 tgcggagcgg agagccaatg gatcatcgaa ttggtggccg tcggtgtctt ggagccagag
      541 gggcccgaga cctcgaactg gcgatttcac gcggcggatc tcagctgcgc ccgccgagtg
      601 gcgcgactcc agcgcgattt cgatgccagc ctcgatgccg cggcagccat gctcgatctg
      661 atcggacaga tcgaacagtt gcgcgcgcgc ctgaagcagg ccggcctcga tgcggatgag
      721 gccttcggcg gcaaggacac atgagacagc cgatacacga gctcgaacgg acacgtcggc
      781 aatcctcggg tcccggtggg gacgaagcca ccgaggggaa taccggtgcc cccatcaggg
      841 catccggctc tcacccctcg acctgggagc ggaagtcatg acgacctcca tgacccgcga
      901 acgaggccta gagctgttgc gacacatgat acggatccga cgcttcgagg aacggaccgc
      961 ggaactgtat caactcggaa agatccacgg gtttctccat ctgtacatcg gtgaagaggc
     1021 tgtggctgcc ggctccatcc cgtcgttcac gaacgaggac gccatcgttg ccacctatcg
     1081 ggagcacggc catgcgctgg tgcgcggcac atcgatgaac cagctgatgg cagagctgta
     1141 tggcaaagcc gctggttgcg cgcgcggacg cggagggtcg atgcatttct tcgatgcttc
     1201 ccggcggttt tacggaggcc tggccatcgt ggccggtgga ttacccgtgg ccgtcggcct
     1261 ggccctcgcc gacaagatgc aaggtcgcac ccgtgtcacc ggctgctact tcggtgacgg
     1321 cgcggtggcg gagggcgagt ttcacgaatc cctcaacctg gccgccttgt ggaaactccc
     1381 ggtgctgttt ctctgtgaaa ataacctcta tgcgatgggc accgccctgg cccgcc
//
LOCUS       DAORRQ010000214         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1636_length_1311_cov_1.969745, whole genome shotgun sequence.
ACCESSION   DAORRQ010000214 DAORRQ010000000
VERSION     DAORRQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1636_length_1311_cov_1.969745"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>619)
                     /locus_tag="PK224_21740"
     CDS             complement(<1..>619)
                     /locus_tag="PK224_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB18428.1"
                     /translation="IGFAVMSTTLTLAAVFFPIIFLPGIVGRLFAELGIAVAGSVLLS
                     GFIALTLTPAMCARLLGKTAGTASPSRGSALDASAWLRWLTQRYRLALKVALERPAII
                     LGGAVLSLIVSAALMWGLPSELAPLEDTGWFAVHVSAPEGSTLDYTDRYARETENATA
                     RIPEMDSYYTVVARGWRPTLVNRAVTWVTLKDWSDRNRVQQEIVDD"
     assembly_gap    620..674
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<675..>1311)
                     /locus_tag="PK224_21745"
     CDS             complement(<675..>1311)
                     /locus_tag="PK224_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRB18429.1"
                     /translation="LRTQNVSMPTGRIESTQREFSVRMGGGLEKAAQFNQLILAYRDG
                     YPVRLQDVGAAEIGAEDDRKLVRVNGKPAIGLGIMKQSKANTLEAVKRTIPAMEALLP
                     EGMQLVTAFDSSIYIERSLHEVYEAVGLSVLLVVLVVFLFLRSARATLIPAVAIPGSI
                     LGTFVIMQMTGSSINTITLLGFVLAIGLVVDDAIVVVENVYRRIEQGLNPTQ"
BASE COUNT          244 a          372 c          407 g          233 t
ORIGIN      
        1 cgtcatcgac gatttcctgc tggacgcggt tgcgatcgga ccaatccttg agcgtcaccc
       61 aggttacggc ccggttgacc agggtcggcc tccagccccg cgcgaccacg gtatagtagg
      121 aatccatttc gggaatgcgt gccgtggcgt tctcggtttc cctggcgtaa cggtcggtgt
      181 agtcgagagt agagccttcc ggcgcactga cgtggacggc aaaccatccg gtatcttcaa
      241 gtggtgccag ttcgctgggg agaccccaca tgagggctgc gctgacgatc agtgacagga
      301 cggctccacc taggatgatc gcgggccttt ccaaggcgac tttcagggcg aggcgatagc
      361 gttgagtgag ccagcggagc caagccgaag catccagtgc ggagcctcgg gacggcgagg
      421 cggttcccgc agtctttccg agcaaccgcg cgcacatggc gggggtgaga gtgagggcga
      481 tgaacccgga caggagcacc gacccggcga cggcaatccc cagttccgca aacaggcggc
      541 ccacgatacc gggtagaaag atgatcggaa agaagaccgc tgcgagggtc agagttgtgg
      601 acatcacggc aaacccgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnncctgtg tcgggtttaa gccctgttcg atgcggcggt agacgttttc
      721 caccacaacg atggcgtcgt ccactaccag tccgatggcg agcacgaatc ccaggagagt
      781 aatcgtattg atgctgctgc cggtcatttg catgatgacg aaggtaccca ggatggagcc
      841 tggaatggcg acggcgggga tgagtgtggc gcgggcgctg cgcaggaaca ggaacactac
      901 cagcacgacg agcagcacgg acaggccgac ggcctcgtac acttcgtgca gcgaccgctc
      961 gatgtagatc gagctgtcga acgccgtgac gagctgcatc ccttccggaa gcaacgcctc
     1021 catggccggg atggtgcgtt tgaccgcttc gagcgtattc gccttcgact gtttcatgat
     1081 gccgaggccg atggccggtt taccgttcac gcgcacgagt ttgcgatcgt cttccgcgcc
     1141 gatctctgcg gcacccacgt cctgcagccg gaccggatag ccgtcccgat aggccagaat
     1201 cagttgattg aactgcgccg ctttctccag gcctcccccc atgcgcacac tgaactcccg
     1261 ctgggtgctt tcgatccggc cggtgggcat ggagacgttt tgtgtccgga g
//
LOCUS       DAORRQ010000215         1308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1639_length_1308_cov_5.143655, whole genome shotgun sequence.
ACCESSION   DAORRQ010000215 DAORRQ010000000
VERSION     DAORRQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1639_length_1308_cov_5.143655"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PK224_21750"
     CDS             complement(<1..732)
                     /locus_tag="PK224_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRB18430.1"
                     /translation="MRDTLPTELETSAGDPRKALLQVAEAISLHRDLPALFRDLAQRL
                     PAVAPFDFIGLVLHDPAKQVMKVHVLETVEARGVTNRLDGMEIPMEESASGWVWSHQQ
                     PLMIPSLADETRYKPGLGALRSIGVQSLCFCPLTTAMRRLGAIGFGSLRTSAFGDADV
                     EFLNQVAKLVAVAVDNVLHHQALSDERDRLQLLLDVTESIASHRDLTRLLEDLAGRLP
                     QLVPFDFINAVLHDPATDKMRLWLLV"
     gene            complement(844..1173)
                     /locus_tag="PK224_21755"
     CDS             complement(844..1173)
                     /locus_tag="PK224_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18431.1"
                     /translation="MLRRGERVGPGRIVLLLCLLGGSLGTLPAVLAEETAVELNSPEV
                     LRQVLEQQMGKRVKIKLLSGQDLDGKVARVGTQAVVLSELGGMEFFDATLRLDQVAAV
                     VVKTRSK"
BASE COUNT          264 a          418 c          394 g          232 t
ORIGIN      
        1 caccagcagc cataagcgca tcttgtctgt cgcggggtca tggagcaccg cattgatgaa
       61 atcgaacggc acgagttggg gcaaccgtcc ggccaaatcc tcaagcagtc gggtgaggtc
      121 acgatgggag gcgatcgatt ctgtcacgtc cagcagcagt tgcagccggt cgcgctcgtc
      181 gctcaaggcc tggtgatgca gcacattgtc cacggcgact gcgaccagtt tggcgacctg
      241 gttgagaaac tccacatcgg cgtctccgaa cgcggaggtt ctcagactgc cgaatccgat
      301 cgcgccgaga cgccgcatcg ccgtggtgag ggggcagaaa cagagcgatt gcacgccgat
      361 gctccggagc gcgcccagtc cgggcttgta ccgcgtttca tcggccagcg acgggatcat
      421 caacggctgc tgatgcgacc agacccagcc gctggcggac tcctccatcg gaatttccat
      481 gccgtccagt cggttcgtca ccccccgcgc ctcaaccgtt tcaagcacgt gcaccttcat
      541 gacctgcttc gccggatcgt gcagcaccag gccgatgaaa tcgaaagggg cgacagcggg
      601 cagccgttgt gcgaggtctc gaaacagggc cgggaggtct ctgtgtagag agattgcttc
      661 ggcaacctgc agcagggcct tgcgcggatc gccggcgctc gtttcgagtt cggtcggaag
      721 ggtgtctcgc atggggaata gtatggagag tctggatgtc cggacgcaac tggagaatgg
      781 agggctcacc tggtgaagcc cgattccccg gctgtcggaa aaccggaagc agatagcagg
      841 gcgctatttc gaccgggtct tcacgaccac agcggccact tggtcgagcc gcagagtggc
      901 atcgaagaat tccattccgc ccagctcgct gagcaccacc gcctgcgttc cgaccctggc
      961 caccttccca tcgagatcct gcccggagag cagcttgatc ttcacccgtt tgcccatctg
     1021 ctgttcgaga acctgccgta ggacctccgg actattgagt tccactgccg tctcctcagc
     1081 caacacggca gggagcgtgc cgagagaccc gcccaataaa cagaggagca gcacaatcct
     1141 acccggcccg actcgttccc cacgacgcag catacatacc tccttgaatt gcccacatcc
     1201 cggctaatgg atcgaagacc attgccccga agcagttcat gccgcgggga ggatagccgg
     1261 ggttccggag aaagtcacgc gcggccgacc gggcccgcag acctacct
//
LOCUS       DAORRQ010000216         1208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1765_length_1208_cov_7.197745, whole genome shotgun sequence.
ACCESSION   DAORRQ010000216 DAORRQ010000000
VERSION     DAORRQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_1208_cov_7.197745"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..>1208)
                     /locus_tag="PK224_21760"
     CDS             complement(241..>1208)
                     /locus_tag="PK224_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRB18432.1"
                     /translation="SVGPKNSFLHLKLAYHGDTVGAVSVGNIELFHARFKALLFPTLE
                     VDPPYCYRCPLRLTYPACRMACIDPLESLLKARHRELAGVILEPLVQAAAGMLTAPPG
                     YLTRVRELCTKYNVLLIADEVATGFGRTGTMFACQHEGVTPDLMAISKGMTGGYMPLA
                     ATLATEEIYRAFLGRYDEWKTFFHGHSYTGNPLGCAVALANLDIFRREKTLLHVRKQT
                     KLLVRLLRPMTLLSHVGEIRQCGLMVGIELVLDRTTRTPYPLEMRIGHRVAQACRKGG
                     LLMRPLGNVIALVPPLSTSSQELTKMVAILHSAIVDVTEGSASPA"
BASE COUNT          271 a          357 c          364 g          216 t
ORIGIN      
        1 cgcctgagcc gtcaagggga gggccaaaag caggagccag ccccacaaca tcagacggcc
       61 tgtcatgctg aaaaatccgt cgagaggatg tcgtggcgca gcatgaaatc aagtctagca
      121 ctcgattctc agccgcccgg gcgccggcgg gggtgcacaa aaaccgtcga gggatgttca
      181 tcggcgaaca cgtgacgccg cgttccagca ggcgggaatc agccgcaatg acgggacctt
      241 ctacgccgga gatgcactgc cttcggtcac atcaacgatg gcgctgtgca aaattgccac
      301 catcttggtt aattcctgag agctggtgga gaggggaggg accagtgcga tgacgttgcc
      361 gaggggtctc atcagaagcc cgcctttccg gcaggcctgg gccacgcgat ggccgatacg
      421 catttcaagc ggatagggag tgcgcgtcgt gcgatccagc accaattcga ttccgaccat
      481 caaaccacat tgccgtatct cgcccacatg actcagcaag gtcatcggac gcagcagacg
      541 cacgagcaat ttggtctgct ttcgaacgtg caggagcgtc ttctcgcgac gaaagatgtc
      601 gaggttggcc agggctacgg cgcagcccag cggattgccc gtgtaactat gcccatggaa
      661 gaatgttttc cattcgtcat accgtcccaa gaaggcgcga taaatctctt ccgtcgccag
      721 ggtggctgcc aagggcatgt atccgccggt catgcccttg ctgatcgcca tgagatccgg
      781 cgtcacacct tcatgctgac aggcgaacat tgtgcccgtg cggccgaagc cggtcgccac
      841 ttcatccgca atcaacaaga cgttgtactt cgtgcagagc tcacgcacgc gggtgaggta
      901 tcctggcggc gcggtcaaca tccccgccgc cgcttgcacg agcggttcga gaatgactcc
      961 ggccagttcg cgatgacggg ccttcaacag cgactccagc ggatcgatgc aggccatgcg
     1021 acaagcggga tacgtcaggc gcagcggaca acgataacaa tagggcggat ccacctcgag
     1081 cgtcggaaac aggagggctt taaaccgcgc atgaaagagc tcgatgttcc ccacgctgac
     1141 ggcaccgacc gtgtccccgt ggtaggccag tttcaaatga agaaaggagt tcttgggtcc
     1201 tacggacg
//
LOCUS       DAORRQ010000217         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1825_length_1160_cov_306.851584, whole genome shotgun
            sequence.
ACCESSION   DAORRQ010000217 DAORRQ010000000
VERSION     DAORRQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_1160_cov_306.851584"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1138
                     /locus_tag="PK224_21765"
     CDS             194..1138
                     /locus_tag="PK224_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRB18433.1"
                     /translation="MSAEIFVGMDVSQGCVDVAVRPGTAFQITQDERGIAEAVERLQA
                     VQPTLIVLEATGGLEVPLTGALAAAGLPVVVINPRQVRDFARATGQLAKTDRLDAQIL
                     ARFAEAIRPPVRPVPDEQTQALAALVARRRQLIEMLTAEKNRLRLAARPIQKRVQAHI
                     TWLEKELAATNTDLTATMRESPVWREKEEVLRSVPGVGPVLTTTLFANLPELGTLTRK
                     EVAALAGVAPFPRDSGTLKGRRAIWGGRAHVRAALYMAALVATRRNPVIRVFYQRLCQ
                     AGKAKKLALTACMRKLLTILNAIVKSGTPWRVAVSQSA"
BASE COUNT          210 a          341 c          399 g          210 t
ORIGIN      
        1 caagaccttg tatctataga ggaggttttg catcatggca cgacaggagc gggacagatc
       61 gcaaagatcc gggtccgtat cattgaagac cgtgggtcag ctcgggtcgt cccgttcctc
      121 cggtcaccaa cccggacagt tgaaggggcc tcaagcccgc attgtgcaag gacgggagac
      181 acggaggtgt accatgagcg ccgagatctt tgtgggcatg gatgtgtcgc aaggctgtgt
      241 cgatgtggcg gttcgtcccg ggactgcctt ccagattacg caggatgagc gagggatcgc
      301 ggaggccgtg gagcggctcc aggccgtgca gccgaccttg atcgtgctcg aagcgacggg
      361 cgggcttgaa gtcccgctca cgggggcact ggccgccgcc ggactgccgg tcgtggtcat
      421 taatcctcgt caggtgcggg actttgcgcg ggccactgga cagttggcga agacggatcg
      481 gctcgatgcc cagatcctcg cccgattcgc cgaagccatt cgtccacccg tccgcccggt
      541 gccagatgaa cagacgcagg cgttggccgc gctcgtcgcg cgccgtcggc agctgatcga
      601 gatgctcacc gcggaaaaga atcggctgcg cctggcggcc cggccgatcc agaaacgggt
      661 gcaggcgcac atcacctggc tggagaagga actcgccgcc accaataccg atctcacggc
      721 gacgatgcgc gagagtccgg tgtggcgcga gaaagaagag gtcttgcgaa gtgtgccggg
      781 cgtgggcccg gtcctcacca cgacgctctt cgcgaatctt cctgagttgg gtaccttgac
      841 gcgcaaagaa gtggctgcgt tagcgggcgt cgcgccgttc ccacgcgaca gcggcaccct
      901 caaagggcgt cgcgcaattt ggggcggccg tgcccacgtg cgagcggccc tctacatggc
      961 ggccctggtg gccacgcgcc ggaatccggt gattcgggtg ttctatcagc ggctttgtca
     1021 ggccgggaaa gcgaagaagt tggcgttgac cgcctgtatg cggaagctgc tcacgattct
     1081 caatgcgatc gtgaagagtg ggacgccctg gcgggtcgcg gtcagtcagt cggcttgatt
     1141 ttcaagacag ttgctgaccc
//
LOCUS       DAORRQ010000218         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_5339
            NODE_1933_length_1078_cov_6.140762, whole genome shotgun sequence.
ACCESSION   DAORRQ010000218 DAORRQ010000000
VERSION     DAORRQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867556
            Sequence Read Archive: SRR5715943
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:05:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,303
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1933_length_1078_cov_6.140762"
                     /isolate="AMR_MDS_5339"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2015-01-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK224_21770"
     CDS             <1..382
                     /locus_tag="PK224_21770"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HRB18434.1"
                     /translation="SDDYPFAELIALNVKDHITDRGCALNQDPDSDEICVPDKNNIQK
                     PKKLDIDFPRCFKHKNRIVGLFTGGKTFHCGVYHPTGNCIMRNSDSDGKEFCPVCRYL
                     LVDIIDPHKHFSIDLDYGEIYPQP"
     gene            379..>1078
                     /locus_tag="PK224_21775"
     CDS             379..>1078
                     /locus_tag="PK224_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRB18435.1"
                     /translation="MSTTAGTFEQVARAMIDALAPLRRALGSTDAFQAFMLRLGWDTV
                     AVPPAYTQLGTAIADAIQAVEALRDDPTENEILVLLGKAKATYLAINRIDQAPPDVDA
                     GTFLSQIGERLFEILLTDYLAAQQPALFNLLSILNVIETAIIPATAHQRGHIRTHFHW
                     ANIPDVIRDPLSLPQKVYGWGTQDLKFPLLLQHLSELCFAFGFPVFIGKAEVSLANGY
                     AGQDVELDEDSHWLK"
BASE COUNT          246 a          336 c          282 g          214 t
ORIGIN      
        1 atcggacgac tatcccttcg ccgaattgat cgccctgaac gtcaaggacc acatcacgga
       61 cagaggttgc gcactcaacc aagaccccga cagcgacgag atctgtgtgc cggataagaa
      121 caacattcaa aagccgaaga aactcgacat cgatttccca cgctgtttca aacacaagaa
      181 ccggatcgtc ggattgttta ccggcggaaa aacattccac tgcggggtct atcaccccac
      241 gggcaactgc atcatgcgca actcagacag tgacgggaag gagttctgcc ccgtgtgccg
      301 atacctgctg gtcgatatca tcgatccgca caaacatttt tccatcgatc tggattacgg
      361 cgagatttac ccgcaaccat gagcacgacg gccggcacat tcgaacaggt ggcgcgcgcc
      421 atgattgacg ccttggcgcc gttgcgccgc gccttgggca gcaccgatgc gttccaggcc
      481 ttcatgctcc gcctcggttg ggacaccgtc gccgtccctc cggcctatac ccaattgggt
      541 actgcgattg ccgacgcgat acaggcggtc gaggccctgc gagacgatcc gacagagaac
      601 gagattctgg tcctgctcgg caaagccaaa gccacctatc tcgcgatcaa tcgaatcgat
      661 caggcgccac cagacgtcga tgcgggcacg tttctgagtc agatcggcga acgactgttc
      721 gagatcctgc tgacagacta tctcgccgcc cagcaaccgg ccctgttcaa tctgctctca
      781 atcctcaatg tgatcgaaac ggcgatcatt ccggccacgg cacatcagcg cggccatatc
      841 cggacgcact tccattgggc caatattccc gacgtgattc gcgatccgtt gtctctgccg
      901 caaaaggtgt acggatgggg cacgcaggac ttgaagtttc cattgctgct ccagcacctc
      961 agcgaactgt gcttcgcatt cggctttccc gttttcatcg gcaaggcaga ggtatccttg
     1021 gccaacgggt acgcaggcca ggacgtagaa ctcgatgagg actcacactg gctcaagg
//
